LOCUS       DAOSYS010000001        51331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_547_length_51331_cov_5.555484, whole genome shotgun sequence.
ACCESSION   DAOSYS010000001 DAOSYS010000000
VERSION     DAOSYS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 51331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51331
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_51331_cov_5.555484"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PLU99_00005"
     CDS             <1..1441
                     /locus_tag="PLU99_00005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO15463.1"
                     /translation="MRSTRVMNIYRHPKPDAPNENPPMAKRTRPAPPANTNGNAWQEL
                     GLQPDLLEGVRLAKFGEPTEIQRELIPVILAGRDCLGQAKTGTGKTAAFALPILQLTT
                     AGEPTQALVLVPTRELAAQVDSHIRQLGVRRPLKTALLYGGRRIAQQVNQLKRAPEIV
                     IGTPGRILDLMQRRELNISDVRFIVLDEVDRMLDIGFRDDIRRILKAIKSEHQTIFVS
                     ATIDDEIRTLARPFTRDAVEIDVSRDTLTVENINQGFVSVDAHHKFETLLGFLKHEKP
                     RLAIVFTNTKHAARRVAERLKHAGVNCKEIHGDLMQSRRERVMKSFRAAQIQVLVATD
                     LASRGLDVMEVSHIVNYDIPEDPAGYVHRVGRTARMGKSGYAVSFVTPDQGKLLTEVE
                     KLINRELPQFDEHWVMKPRRAPETAAAGAAPEAASGPQPAGDSAAAAGPVPTRYREAI
                     RRCAILESHGLKPLRRTLGSRFRSTRRRR"
     gene            1526..3277
                     /locus_tag="PLU99_00010"
     CDS             1526..3277
                     /locus_tag="PLU99_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15464.1"
                     /translation="MPEYRAAFCGGARTDAGSTGLRSGRGFVYRQDSVAARPGSASLK
                     IADRILLLALSLVLLLPGTWVSLTDRDEGYYGQVCREMLAGGDWLVPQYLGQPWLFKP
                     PLLYWAVSGAFTIFGLQEWAARLVSVLALTGAVQFLAALAADMYNRRTALIACACFLT
                     AGLPLIVGRMLLTDPLLLLWMMAAVWLLWRIATRSAGAALSAGFWLSLGLGVMTKGPT
                     ILLFMGAFGLALLLQPGRRKWIVSGRFWMMSPLIALAAPWFIYIAQRHGDLFTQRFLF
                     AEIVLRMVEPSCGHVGPPGYYLLLSLAGLLPWTALVPGAVAETWGNRRADRAAPLLLV
                     WCGLPWLILELMGTKLPHYVLPCYVPLAIMLGRMWDAGAGRELLPGQRAVLRLWAAVP
                     IVIGLGILTAAVFYHSAAWARPAGAAGLVLVAGFWLAARAACRRPLAAYRRAVASMAL
                     FSIVAGAWLLPSLEPHRLSRVVAEKANGCNSGMPVIVGGYEEPSLFFYLAQPPQVLKG
                     RDLADGLADLKRPVVAILGASELTAAGLAESAAREGWTSVTGFNYSYGRTETIWVGHL
                     DPRERSPGASRPTVSGG"
     gene            3422..5065
                     /locus_tag="PLU99_00015"
     CDS             3422..5065
                     /locus_tag="PLU99_00015"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOO15465.1"
                     /translation="MSQLFSSQFFTLFAIIALGVTLGSFRLGGIALGSAGVFFVALVF
                     GHFKCTVSHELTELGLVLFVYAVGLQAGPRFFSVLRRRGAAFLAVGLGSTAVGALAAI
                     GLAKWFGLSANLAAGLYSGATTCTPGLAAALEAVRRVTGDPGSAETIEAVNATSVGYG
                     AAYPFSVVCVVLLVQLLPRLLRTPAKTAAAAFRAEEETHTPPLDECAFRVTNRNCAGR
                     TVEELQALHLSNAVLCRIKHEGEIYPVKPETTLHLGDTVLAVGTPEELAKLEAVLGDV
                     VVEPMQDPSGKVTSELLLVSRKDVVGKSFRELCIWERFGVVVTRLRRENVELTPRGDV
                     RLEPGDVLRVVGTRRDIRALADALGREERRLAETSFVPFAAGIALGAAVGQIPITLPG
                     GLEVRLGMGGGAFLVALLLGHLGTIGPVRVYVPNAAKYFARELGLVIFLAGAGAGAGQ
                     RFVPILREAGGQLILAGALVTLATVVTALFIIYALCRWNLLYGAGALSACMTNPPGLA
                     AATALTDSDAAPVGFASVYPVALIAKILFAPLVYLLLRM"
     gene            5385..6512
                     /gene="tal"
                     /locus_tag="PLU99_00020"
     CDS             5385..6512
                     /gene="tal"
                     /locus_tag="PLU99_00020"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOO15466.1"
                     /translation="MINRLQQLAALGQSVWLDNLSRDLLDSGRLAELVDEGLAGVTSN
                     PTIFHKAIAGSKTYRPEIQRLARAGKTAMEIYEELVIRDVRDAANVLRPVYNETHGRD
                     GFVSLEVNPKLAYDTEGTIAEARRLFRAVDRPNLMIKVPATEEGMPAIEALIAEGVNV
                     NATLIFAIPMYERVMQAYIQGLRRLIPTGKPLGLVSSVASFFVSRVDTLTDEVLQHRI
                     THGREEHLTPLFGQAAVANAKLAYARFKQVFIGEPFADLRARGARPQRALWASTSTKN
                     PSYPDLKYFNPLIGMYTVNTLPMQTLEAVLDHGLAAQTIEDGLAHSEAVMRELAGVQV
                     EMEWITGCLLKQGVEAFTESFDQLIAEIEAQRAAALSRQPA"
     gene            complement(6616..7038)
                     /gene="rplL"
                     /locus_tag="PLU99_00025"
     CDS             complement(6616..7038)
                     /gene="rplL"
                     /locus_tag="PLU99_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOO15467.1"
                     /translation="MAEAPARTFDAAIKELGDRIAGLTLVQARDLTDYLKEAYGIEPA
                     AGGAVVMAGPAVGGAGAPGAPAAEEKTEFNLVIKSAGDKKLQVIKVVRAARSDLGLKE
                     AKELVDTAPKPLLEGVSKDEATKWAKQLEEAGATVAIE"
     gene            complement(7149..7730)
                     /gene="rplJ"
                     /locus_tag="PLU99_00030"
     CDS             complement(7149..7730)
                     /gene="rplJ"
                     /locus_tag="PLU99_00030"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOO15468.1"
                     /translation="MSKFVKDLITKELRSRYSQTDNAIWVEMIGIDGITTNQFRRELR
                     EKKLRLEVVRNALLRRAVGDGPLSRLADKLDGPAALITGGDSAVEAAKFLDQWLPRLK
                     GMRLRGALLEGEFLDEAAVAGLSKMPTKRDMQARLASCVRSPGANLASAMLAGGRNIA
                     GCLKSLIEKLEKAPPPAAEAAPAAAEPAPAPAE"
     gene            complement(7735..8436)
                     /gene="rplA"
                     /locus_tag="PLU99_00035"
     CDS             complement(7735..8436)
                     /gene="rplA"
                     /locus_tag="PLU99_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOO15469.1"
                     /translation="MPQLRGKRYRQDEKKRPAKPVPLEDAIKTLKTFSKTKFDQTVDL
                     VAHLGIDSRQAEQAIRGSVSLPKGIGATKKVIAFCGDADAEAAKKAGAIEAGGDELID
                     KVQKGWMDFDVAIAHPSMMGKVGKLGRVLGPQGKMPSPKSGTVTPDIATAVREYAAGK
                     VEFRNDKGGNLHAVVGKLSFSEADLKENVVAFMDHIRRLKPPTAKGQFIKKLALSGTM
                     TPSVLVDTSTGSGLE"
     gene            complement(8544..8993)
                     /gene="rplK"
                     /locus_tag="PLU99_00040"
     CDS             complement(8544..8993)
                     /gene="rplK"
                     /locus_tag="PLU99_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOO15470.1"
                     /translation="MAKAVVAKIKLQAPGGKATPAPPVGPALGQHGVNIGQFVSQFNE
                     RTKHMDGMTCPVEITVFKDKTFEFVVKSPPAAALLIKAAKPTAEGAAIVEKGSGEPNK
                     TKVGRVTRDEVRKIAETKLKDLNAFDLDAAERIIAGTARSMGIEVID"
     gene            complement(9293..9940)
                     /locus_tag="PLU99_00045"
     CDS             complement(9293..9940)
                     /locus_tag="PLU99_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOO15471.1"
                     /translation="MSEDSLELLAGRARVVRFEKGRIIFRQGEECPGLYCVGQGLVRV
                     YKIAPTGKDHVLHFAEPGMTFAEVAVMSNFSCPAHAEALEDTVCAFVQADDFRRLLRS
                     SHEVCLQLVEGMGFWVRQLVGLLEDIVLRDATARVAGYLLRTDPSGGRAAFTLPTLKK
                     DLASHLNLTSETLSRTLRRLAEAGLIETPDAQQIRICDFAALQDISQGLMPAEFT"
     gene            10176..10988
                     /locus_tag="PLU99_00050"
     CDS             10176..10988
                     /locus_tag="PLU99_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO15472.1"
                     /translation="MLREVVTIDEELCDGCGLCVPACAEGALRIIDGKARLVADRLCD
                     GMGACLGHCPRGAIRIEHREAEEFDEAAVVAAAHAPQTRTAEPATARAAACPAPSHSH
                     AGGCPGMRLNVWDVASGNGHAARPAAASRPEKAAAPSALTHWPVQLRLVPPTAPMLRG
                     ARLLVAADCVPFAYAGFHADLLAGRAVVVGCPKLDDRQEQIARLSAIIAQNDLAEITV
                     AHMQVPCCTGILHAVLEARQRAGSSVPVVSVVIGIHGDILERREVVAAGAEF"
     gene            11026..12330
                     /gene="hcp"
                     /locus_tag="PLU99_00055"
                     /gene_synonym="priS"
     CDS             11026..12330
                     /gene="hcp"
                     /locus_tag="PLU99_00055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOO15473.1"
                     /translation="MFCNQCEQTRGSGCVEVGVCGKNADVQSLQEILLYGLKGMAAYA
                     HHARRLGATDESVSAFIEEALFATMTNVNFDVPTLFEMALECGRKNLRVMEMLDEAHV
                     QRFGSPSPATVYEGTKAGPGILVTGHDLLDLEDLLKQTEGTGINVYTHGEMLPAHMYP
                     GLRKYKHLAGNYGGAWQRQKVEFAEFSGPIVATTNCVLIPREEYADRLFTTRVTAVPG
                     AKRLTSNDFSAVIECAKKCAPLPEERVRESTVGFHQSVILGLADKIVAAVKAGKISHF
                     FLVGGCDGYEGARNYFSDFAQATPKDSLILTLGCGKFRIRDNDYGTLDLPGVGPIPRL
                     LDMGQCNNAYGAIQVAVALAKAFDCGVNDLPLTLAISWFEQKAVAIFLTLLALDVKGV
                     RLGPNPPAFVSSNVFRILQDKYNLRLTNGHAAADVQAALAHK"
     gene            12357..13103
                     /locus_tag="PLU99_00060"
     CDS             12357..13103
                     /locus_tag="PLU99_00060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO15474.1"
                     /translation="MAGQLFERHVELYDALIDWPKRLANEGPFLRAVFDRCGARRVLD
                     AACGTGQHAAMFHSWGLEVEGADVSPAMIARCRQQFGEPGTLRWTVRGFEEPAPAAVD
                     AVVCLGNSLALAGNRGGAQRAVAAMLASLRSGGFCVIQVLNLWSLPEGPCIWQKFRRA
                     RVDGREHLLAKGVHRHAGAGFVEFLALPVGADEEPSYETVRLTGLTREDLAAFASAGG
                     ARQVEFFGNYARDAYEPQRSVDLIAVVEKA"
     gene            13103..13516
                     /locus_tag="PLU99_00065"
     CDS             13103..13516
                     /locus_tag="PLU99_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOO15475.1"
                     /translation="MRDSGQQAEHSRTRQAAAGPMIDRKLRKDLRTLALFIELYCRKR
                     HGPMPRAAVRLRTLDVAGVHGRPLELCPDCAKLAAHAFAKRMQCPLDPKPACKHCPQH
                     CYADQYRRQIREVMRYPGRRLVLSGRLDYLLHLLF"
     gene            13604..14056
                     /gene="nrfH"
                     /locus_tag="PLU99_00070"
     CDS             13604..14056
                     /gene="nrfH"
                     /locus_tag="PLU99_00070"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOO15476.1"
                     /translation="MNAVKLAAFAGLGLLGVGAGVGAYTFIYAEGASYLTDDAAACAN
                     CHVMQEQYRAWNRSSHHAVATCNDCHTPHDFLGKYLVKARNGYHHSLAFTTGWFHEPI
                     QITPQNLAVTEAACRRCHQDIVQAIDTSQPGQPPLSCTRCHPNVGHLH"
     gene            14156..>15543
                     /locus_tag="PLU99_00075"
     CDS             14156..>15543
                     /locus_tag="PLU99_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOO15477.1"
                     /translation="MANPVEPPKDVSYTNPPRRGRTLLIAAALALVAAATFGVTALLV
                     NIFQHKQEARNPFFRVVELDDNTVDPQIWGKNFPQQYDGYARTVDMQRTRHGGSEALP
                     HEPSEKDPRTKVAQSKIELDPRLKTMWAGYAFAKDFREERGHAYMLEDQTYTQRVLEF
                     AQPGTCLNCHASTYVAYKQLGDGDIMAGFDKMNQMPYAEARQLVDHPISCLDCHDPQT
                     MQLRVTRPAFIEGIRVVKAAEGVPDFDVNRDATRQEMRTFVCAQCHVEYYFKGKEKRL
                     TYPWFKGRKADEILAYYDEVGFTDWTHAETGAPMLKAQHPEFEMWSQGIHARAGVTCA
                     DCHMPYMRVGAMKVSDHHVRSPLLNIANACQTCHRVPEQELLARAETIQSRMLFARDL
                     ALDALMELIDDIKDARQAGATDEELKAPRDFQRKASFLVDFVEAENSAGFHADQEAVR
                     VLMQSLEYSRQG"
     assembly_gap    15544..15643
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15648..16124)
                     /locus_tag="PLU99_00080"
     CDS             complement(15648..16124)
                     /locus_tag="PLU99_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOO15478.1"
                     /translation="MDFTLERILWPTDFSELSLKAGRYAYALARHFGARLHVVHVIPP
                     IMTADFSVALPATLPVTAADPETIQACRKGLQRIITERLQATDDVQQEVLFGNAWSAV
                     CEYAAAHQIELIVVSTHGRTGLTHIVIGSTAERIVQHAPCPVLTVKASGRDFVAPE"
     gene            complement(16212..18191)
                     /gene="gatE"
                     /locus_tag="PLU99_00085"
     CDS             complement(16212..18191)
                     /gene="gatE"
                     /locus_tag="PLU99_00085"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOO15479.1"
                     /translation="MSLRFKPFEEMTRDDYAQIGLKCGLEVHQQLLTETKLFCRCPAG
                     RYSDDYDTEILRHMRPTLSELGEYDGTALMEKKTRKNIYYRIHHSTVCTYEFDDTPPF
                     LIDDDALDITLELCLLFDLNIVSELHIARKQYLDGSIPAGFQRTGIVGVTGALPYGDR
                     TIGIRQLSIEEDACREVSDVGHDRVYLTDRLGMPLIEVVTEPDMCTPDEAADVCQLIR
                     RICRSTGKVRTGYGAARQDVNVSVAGGTRVEIKGVPQIWRIPRLVYNEAHRQCALLEI
                     REELRRRGVTPETFQYSAHDVTRLTERVQWEPVRAAVEAGLRVKCVVLKRFGGLLNTV
                     TQERTTFAKEFADRVRVIACLTRLPNITHSDTATEALAARDWENLRKRTRAAADDALL
                     LVWGSEEDTETACNEIAIRAREATLGVPSDTRQPLKDGTTGFERVLPGAARMYPDTDL
                     PPLAISAQRVARIRAGLPERVWNREQRLRELGVPGDTLVPLLTSRHGELFFRLVRDLK
                     VDAKLAAVVLVQRLKAWRRAGLDVNDLSDADIYAAFEAYANRRLTREGLLDVLEYVLG
                     NHFAPCDSAARVQAAMNALGYGHVAEGDPSALVSSTVDGLDSSKFADAGDMQRYAMGV
                     LMRDLRGRVDGRRVAELVSAALRQKSRAMAKVDQG"
     gene            18441..21437
                     /locus_tag="PLU99_00090"
     CDS             18441..21437
                     /locus_tag="PLU99_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15480.1"
                     /translation="MPQRPQDHPEAVLQHEIESYRRKLALQMAGRRLLVLLAIAAVPP
                     LLFVVADHSHPAGLSRSTALLFARCWCWFLGLSSLTLLVLTFRRRLNPLYAARALERA
                     SGIDHNSIVNAVLLQRQPRAAYASTAAAEQAAADLSEHWPTRIGESGAMRLPLTLITV
                     VLAAWIGYALLASKPVLPSVARFFGASRPAPTATTLEQVRPRPGEAVHAGQELRLEFA
                     ARGGSVRQVTFEILAADPDSGDHAMRYELKPVTEPAANWQLTLAPHEVNRDIHYRCTA
                     NDAVLTGTIPVLPVPMLTGLEIELEPPAYSGLPIQITANPDLEVLAGTRATFRAFANT
                     VVENGTFVLTNGHETRTRMNVDPADPYRFTLSMVLVEGGQYRIEFHDRLGAAVKDPPA
                     HQITLRPDEAPTVTIVEPAEADMPGGVVDVTRHAALVAVAEDDVRLAEFELVRADAGA
                     LARNILKMELEADGHRGRVRVPTADLPMKPGQQMRAWFEVRDGRTRSDGTPAPQTGRS
                     REIILSRPAEAVRGRPSLPKRQTASAQPPPPEEATPDEHSASRPAGNEPAANQPTAGA
                     SGGQTGDQAGSAGPDAGKTGGTGAEATSSSPSNEGDFERDLQRFVEENGGMARDLGRR
                     IPKPEPEAGPDGGESQNSGQTEPAGGASPEQTDQTPPAPSPASGQDEPAAQPPQSPRP
                     EGTPPAPTAPPPQPDGSPSANRGQEKPGEDESNQAGGGSQQQPQAPDPAGGASPERAG
                     QTPPAPSPASGQGEPAAQPPQFPQPDGSPSANRGQEEPGEDKSNQAGEGSQRQPQAGG
                     DQPGQGSGQGAGDKDRPPSDSGSTQSGGGTGSGHADSPAGNGTVALPQAPQPPNWRSR
                     TGPLQAEGLTETLDLLEWMDRGGEVEENMLVDLGWPADKAAAFITELRRLHEQYRRAG
                     GLGELQALRVRLSLGDPNVQAGGGLSSAARTAVDLARPHEDHLRRIAPPPEQEVPAEL
                     RSLLDAYYRALAQRQGRGYVTDPANP"
     gene            21679..>23330
                     /locus_tag="PLU99_00095"
     CDS             21679..>23330
                     /locus_tag="PLU99_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15481.1"
                     /translation="MRFTKFFRRYSRALLMVFMSLLLVTWLITPGSNSGPSRRDDIVI
                     GTSIHGEITSGDWAQAQADQQALSALDSSVMRFDPLDFWLLTKEAQRMGIRVGRAEVQ
                     AALAQSGNPQELQRILAGIQKSSRRSTEGLYDLIGRWLAVQKLLGIQEDAIGESAPRI
                     EMAYRDATQKADVQVSVIDSRAFVNGVAEPTEEELQAFFEANKDRFPGHTDDQIEFGY
                     RLPDRVRIEYVTIDPKSLEPKIHARQRELEKYFQENAWKYTKTVTPETQAAGSTPTPT
                     RVPMTFEEAKDLVRKDFIRERAILEAQSLMNQVRDTVYAPWQTQRPDEQGFRPEPEGA
                     PVSFSDLREQFATRAEVIYQQTPLMSQEELQKYFDDRPDFLAKELADQLGSPEPIYSE
                     GMNRLSASELAFRVKGLYTPQPEERLPVLSLNEPSPVLVTRKTDFSVGMGRPSETPYQ
                     PYIFRVVQAEPSAPPASLDEVREQVVADLKLMKAHAAAGEHARQLAEAARNLGLAAAV
                     EQATELKQLLAAAEPTAPAFLSDTPPAPSAYVTELGPAAPLQP"
     assembly_gap    23331..23340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23341..23679
                     /locus_tag="PLU99_00100"
     CDS             <23341..23679
                     /locus_tag="PLU99_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15482.1"
                     /translation="PVTRASDQLGQYLTDSQKLPREIFEAVEEAGPSSASAHVVVVND
                     VAKLFKWAVVEVEGLKPVYRGEFDQQRPRLHAMSSQLAAQEVLGGWLSPENIHLRNKF
                     VETRATQPAD"
     gene            23681..26818
                     /locus_tag="PLU99_00105"
     CDS             23681..26818
                     /locus_tag="PLU99_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO15483.1"
                     /translation="MGASYNFREIEVKWQRTWARERAARAANPGEPGSERPQFYVLDF
                     FPYPSGAGLHVGHPLGYIASDIIARFMRMRGYNVLHPMGWDAFGLPAEQYAIETGVHP
                     RETTRRNIATYKRQLEMIGLGYDWDRELATCDEEYYRWTQWIFLKIFNSWYDPERRWV
                     DADGRTVVGAARPIEELPIPAEVRARGQQAIEEYQAEHRLAYLAEVPVNWCPALGTVL
                     SNEEVTNEGRSERGNHPVFRRPMRQWMLRITEYAQRLLDDLEGLDWPEPIKQMQRNWI
                     GRSEGAYVDFRLDAPGDLGRQVIRVFTTRPDTLFGATYMVLAPEHRLVPIITTDLHRD
                     IVNAYVAEARQKSELDRTAETKVKTGVFTGAYAINPVNRQRVPIWVADYVLMGYGTGS
                     IMSVPAHDERDFEFAREFGIPIRDVVYPAAVAAMHYFAQAAPAEARAESEWRDVLADF
                     LDAVAAGDVAPPGFAQTLQQVLARRGGPGPAPRASELFADGVLKQLPGVIERELGHTR
                     VAWPDVFEKLGFRSFEDLRDCFQKGEFCTRTGEAYTGLGFNVNSPGETGEHFPGQSNL
                     NGLHTPEAKQRITAWLESCNLGEGTVQYKLRDWLFSRQRYWGEPFPILYRPDGGIEAV
                     PEAELPVRLPDMEDFRPETSDDPNSQPASPLARAKEWQEVERDGVKYRRELNTMPQWA
                     GSCWYYIRFCDPRNAARFIDPQAERYWLGGRTAGGAPQLGGVDLYMGGAEHAVLHLLY
                     ARFWHKVLYDLGYLSTPEPFHKLFNQGMITAYAYQDARGVYIHYNDIDFREDGAYHKQ
                     TGEKLTETNAKMSKTLRNVVNPDHIIEEYGADTLRLYEMYMGPLEASKPWNTRDIIGV
                     HRFLQRVWRLMIQPVDENDPEGAWRINPKLVDKDNEALERLLHKTIQKVGSDIQRFSL
                     NTAIAQMIVWANEAGKAQTVGRGQCARFLKLLAPFAPHISEELWSRLEQPRSIVEDGW
                     PDFDPALVKDDVIEMAVQVNGKVRGRIQAPVDADDAELTRLALQNEAIAKAVAGRPVR
                     KTIVAKGRLINLILA"
     gene            27624..27902
                     /locus_tag="PLU99_00110"
     CDS             27624..27902
                     /locus_tag="PLU99_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15484.1"
                     /translation="MGHLLRPPGDVRVVLREGGPAPCMPEGVIITDLYYARERLSETA
                     PGCDSDDEPGWIILTADVSGPGITPHFNDRIFYVGPPDDLQPVLQSGD"
     gene            27924..28352
                     /locus_tag="PLU99_00115"
     CDS             27924..28352
                     /locus_tag="PLU99_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15485.1"
                     /translation="MYLDMALNFGAAVSDHATILACTDLAGTGVTLFNNYACWTGTRE
                     SLQLAARRGMQVPDCPTGVILGSPARVAHNDAGQFTFGAVLRGTGVTVLNDGGRWMFD
                     PGAGLIKIAREGDPIPWFGGGQSWKVIGGSLGTINVWSDG"
     gene            28403..28837
                     /locus_tag="PLU99_00120"
     CDS             28403..28837
                     /locus_tag="PLU99_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15486.1"
                     /translation="MNVTSAMNDVGDIVGPTCIQGPGVIENVNHVVLWIRHHVLKRWI
                     PLLRSGDQIHGRTVIAGYEIDFGLSPSGGADGAWQNLNDRGMVVDRFSFADGSDGIYR
                     LSLVLADADRDGDLDADDWTRLPACLWTQLARRLVQTAAYLI"
     gene            complement(29143..30918)
                     /locus_tag="PLU99_00125"
     CDS             complement(29143..30918)
                     /locus_tag="PLU99_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOO15487.1"
                     /translation="MYIPALESVLLKPLPIKLQVAQIGSDFEFATISDVEVADLDGDG
                     QLELAVAWFATDQQDASRNARRLVIYRLAAAPIVGLEFTVVADLNLYIPDYEIPALSI
                     FRNGTADIGIGDFDGDGDLDLAVHAFFGDELWFIENLGGASYQQYLMFPFYFNTTGNF
                     ITPPESLAGDFDGDGRDELVYIADPILQIDGQIIHFWKTDDTIANMYRVEWEALEDPV
                     FTQWTRGLAVADFDGDGRSDLAFTGTMEPPYESGPILTIWYGLDPDTRQFHVHHEYPD
                     MLCSDVVAVRPDASRNPGVILSDLDGTKIQYWAGAQGSQVDLTFQFECGGFAGLSPER
                     GMTLVTADINGDGYLGVLTKQKRGHAADANQVQVARYRPASGAWTKVAPDPLNSSGFE
                     NWPYDQILRPRNLAAGDALGNGLPEVFAAFGPSHRGILGRSTGRLEVAIWQNSCAGDV
                     NGDGWVGAPDLRHLYRALGTCRGAAGFNPNADIDKNGCIDLMDLGLLVEDLGCVARDG
                     IIPPCGSQKIGDSNCDGVITVFDINPFIRAVSGGEAAWSAYVGESACHFWCVNDVNGD
                     GKVDAFDIDIFIHCMLPAADLLRGG"
     gene            complement(31443..32240)
                     /locus_tag="PLU99_00130"
     CDS             complement(31443..32240)
                     /locus_tag="PLU99_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15488.1"
                     /translation="MGIDPLCPVGEGGFLMFTPGKSGRCTVSLFIALLGIVAASPLAL
                     AAVSETILTVTATCGRSSATLMITQEGGYWDGDNFFWSIDVPMEIRDGERLLGTFGPA
                     SIAVYADPQVNLGFAVQADEEMTSFTITSALLDFPDIEHAWARADAAFTLQDWGTSGA
                     LLTGTGPGGGAYLTQYNGLVPDGTTFAEGINAIEVVPPAMLQVAEFSSAAPGEYTYIG
                     FAGSMSSQIAFNLSPMSMASGSANFEIIPEPTAMLLILVALAAARRR"
     gene            32827..33702
                     /locus_tag="PLU99_00135"
     CDS             32827..33702
                     /locus_tag="PLU99_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15489.1"
                     /translation="MGIIALLLSITAPTIQVARRQAQQAKCASNLKQLGFALENLRQN
                     YDGFYPLWDDNSGPTRYTWIDILVQTRVLGSHQAGYCPADLKPERNNEARAQFFGLIY
                     PGGGMAPGVDYSYGIGVPLSAGGWRWQPRFSPGDPMRRQFHNYDRYPSEMLLAADACW
                     TSVYNMSGDYLVSGIWNDPTQFDNTISWSRHPRHRANILTQDCHVDTVEYKLDAPEPV
                     NTLKHFVWYPGESRNVNPDYEDPYDEGNAYPNVPPIDWSDTSNDGSSIFPNDLNPRFY
                     TTRQTWTQIHHKTVR"
     gene            complement(33745..34752)
                     /locus_tag="PLU99_00140"
     CDS             complement(33745..34752)
                     /locus_tag="PLU99_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOO15490.1"
                     /translation="MKAMVLERIAPVQTAPLKLMDRPMPQPGVGEVRLRVHCCAICRT
                     DLHVIEGDLPEHKTPVIPGHQVVGTVDALGPGCKSLQLGQRIGVAWLRHTCGTCEYCR
                     TGRENLCEQQNFTGYDADGGYAEYALVPEDFAYPLPAAFADEEAAPLLCAGIIGYRAL
                     RRSNLPPGGKLALYGFGSSAHVVIQIALHRGCQVYVATRGENHQRLAREMGADWVGEN
                     PAEMPVKVDSAILFAPVGTLVGPALENLRKGGTLAVAGIYLTDVPTLQYERHLFYERD
                     LRSVTANTREDGRELLAEAAEIPIRPHVTRYALADANRALQDLKADRIAGTGVLLINH
                     A"
     gene            34999..35469
                     /locus_tag="PLU99_00145"
     CDS             34999..35469
                     /locus_tag="PLU99_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOO15491.1"
                     /translation="MKITVRSTAFEPGKQIPKKYTGEGEDLSPPLTWSGAPAETKEFA
                     LICDDPDAPRPEPWVHWVIYGIPGKLSGLPEGLPNELRLPNLPAVQGKNTWNKVGYNG
                     AMPPPGHGVHRYYFKLYALDAELKLQPGLTKDELLKQIKPHVIAEGELVGTYQR"
     gene            complement(35507..37624)
                     /locus_tag="PLU99_00150"
     CDS             complement(35507..37624)
                     /locus_tag="PLU99_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO15492.1"
                     /translation="MAVGTRTGRNHLSHATSPYLLQHASNPVEWYEWRREALHRARTE
                     RKPIFLSIGYSACHWCHVMARESFEDEATAEVMNENFVNIKVDREERPDLDAIYMQAT
                     MILNRGQGGWPMSVWLTPDLRPFFAGTYFPPQPAFGRPGFRDLCRRIGELWKTRRDDL
                     LAEADRLTEAVIQSLEPGRHGAAALTQEGIDRAAQAVAGGFDRQRGGMLNNGTIKFPP
                     SLALDLFLRLVHRHGPDAAQSRELLALVNLTLDHMARGGIFDQLGGGIHRYSTDPAWH
                     VPHFEKMLYDQALVSRVFVDAHLVTKNPLYRRVACELFDFVLGELQAPGGGFYSARDA
                     DSEGVEGKYYVWTKAEILASLGPEEGELFCDYYGVTAEGNWRDPHAPGEAKNVLHVPR
                     SLEEFARNRGADPAELEPRLAAARRKLLAVRSGRVPPALDDKILVEWNALMIASLARG
                     GAALSERKCVDAAIGGAQFILDHQCRDGRLQRVFRDGRTGEIAFLSDYAAFIEALLEL
                     YEATFDKRWLEQALELNRTVIEQFGDRRAGGFFLAPSNHEPRIARFKDARDGATPAGN
                     SMQLMNLLRLSAITGDRSLRHWADQTMSCFAADVLQFPTAGARFLAAAEFALTGPVEV
                     TVVGDQSSDGAQQLWRHIHSRYLPNRVLLHADPAHPQRNIAAPALQEKRPVDGSPAVY
                     VCRDQTCFPPATTVGQLDELLVR"
     gene            38009..38437
                     /locus_tag="PLU99_00155"
     CDS             38009..38437
                     /locus_tag="PLU99_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO15493.1"
                     /translation="MVAPYSLTPFTELRRQLDQLFDEFGFGRWRAPLMTAAYPLVNIC
                     DVGEELHVEAELPGVKKEDLEIFALGNELTIKGRWQPAAGDGQTRHRQERPSGEFSRV
                     ITLPVEVDEDKVAAELKDGVLSVRLPKVEAAKPRKIMLKS"
     gene            38485..38877
                     /locus_tag="PLU99_00160"
     CDS             38485..38877
                     /locus_tag="PLU99_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO15494.1"
                     /translation="MVDTTLQKREAQRGEAEQIRSGPMFIPAVDIIETNNEILLLADM
                     PGVKPDGMDINYEAGVLTIYGRVEPRRKGDEDYLLEEYEVGDFSRRFEIGEGIDANKI
                     EAELHDGVLTLHLPKTPEVMPRKISVKA"
     gene            38920..39387
                     /locus_tag="PLU99_00165"
     CDS             38920..39387
                     /locus_tag="PLU99_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOO15495.1"
                     /translation="MNLLPRRSRRELSPLSRRDSGLTRFRTEMDNLFNRFLGEPWGFM
                     EGLATPRTDLAETDKHVKISMELPGIDPKDVEISVAGGMLTVRGEKKQEREEERQDYH
                     YVEREFGSFQRTFQLPTSVDPDKVDAQYRNGVLHITLEKQPQAQAKRIPVKTA"
     gene            complement(39618..41003)
                     /locus_tag="PLU99_00170"
     CDS             complement(39618..41003)
                     /locus_tag="PLU99_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOO15496.1"
                     /translation="MLPFGIAIAAALPGTVLRLAGVPLDPALAAATSGAAIVGASFLL
                     LWACDAAQADVSQALALAVVAVLTVLPEYAVDMYFTWQAGQHEGTAYAQYAVANMTGA
                     NRLLIGVAWGLVAALYWWRFRRAVRIGHERCTELLFLGLATAYAFVIPIKGTLAWYDG
                     LVFLGIYVWYIVLASRRPCVESEAVGPAEMLIRLPRRQRRAATAVLFLVAGAVILANA
                     KPFCEGLIGTGRLLRINEFVLVQWLAPIASEAPEFIVALMFAWRGQAAMALGTLLSAK
                     INQWTLLVGMIPGVYALSHGTLEHPIPMNGLQMQEILLTAAQSLLAVMLLASLRLSVS
                     GGVLLFALFVGQLTLPGVVAAHPAAAFGLRPEHIHAIFSAFYIMAAVTLLLENPGKLR
                     TLSRGLSLENDELPTLEPAGLASEDTEADLACGIPASAMEPRTPQCLECRWRRFGLRA
                     LRSKPQAARAR"
     gene            41293..44499
                     /locus_tag="PLU99_00175"
     CDS             41293..44499
                     /locus_tag="PLU99_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO15497.1"
                     /translation="MKLGTAGWVCIWSVVCVSVLPGAVADTLLVPSQYATIQEAIEAA
                     LPGDEVVLADGVYIGPGNTDLDLGGKAITVRSASGDPALCIIYCRGAGRGFYFHSGET
                     SDAVVRDLMIVEGAGEYGGGIYCENASPTLIDCVIISNAAELQGGGMYCRDAAPTLIG
                     CRFKGNVSLHQGGGLYCENASPTLVDCTLSGNTADFGGGVGLWESSITLSHCTFTENN
                     YTYGGAIYSSASIVMLDNCSICRNQGNYGAGLYLSASSSATLTDCELSANLARGSGGA
                     VYSRDSSVTLTDCTLTGNRASYGGGALNCEQSSAVLAQCTLVGNTTESSGGAIDFDRS
                     HGTMTACTFVDNTAGRTGGAVYCYESTPIFDACTFSFNTASYQHGGGLYLSYSDATLN
                     NCAVTDNRAQYGAGIYSSRCNPALLNTLIGRNTAADMGGGLELHESNPTLDNCILLSN
                     SAHSGAGLACFSSSPTLTNCTVVGNAASENGGGIYCGGASANPILTNCIVWSNAGGQI
                     SAPYGGNPIVTYSDVQGGWPGTGNINADPKLALEYDGHLLATSPCIDRGTDTPAAGLP
                     AEDFDGNPRPLDGDGDGLASADMGAYEFVPGAPSLAVAPALLRFTIAPGQSGSQTIQI
                     RNSGPDTLAWQVHCDADWLTADPAAGESAGQIDTVTLTADAGDRARGTYIATLWVDAP
                     LACRPHIPLLVVLHVSGALRVPADYPTIEAAILAATPGDVVMLDDGVYTGDGNRELDF
                     GGKAITVRSASGNPATCIIDCGGVAQGFYFRNAEGPDSVLEGVTIRNAHDDYDGGAVH
                     CEYSSPTLLNCWIVSSTTGRSGAGVYARFAEPRLVNCVLVDNSAAASGGGLYAGSDAR
                     PVLTNCTIVGNSAGEYGGGLAFSYSRGTLVNSIIWANSGDQIYRTSSTVSVTYCDVQG
                     GFSGVGNKNADPCFVDAAAGDYRLAAGSPCIDAGKNSAVPADVLDLDGDGNTTEPIPF
                     DVLGQLRFSDDPATADTGSGTPPLVDMGAHEYQRPVSFADFEAFAECMSGPGVTFEAG
                     CGASDFQNDIDVDLADFVWLQRACAQDGGHRAGLTTKN"
     gene            complement(44641..48702)
                     /locus_tag="PLU99_00180"
     CDS             complement(44641..48702)
                     /locus_tag="PLU99_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOO15498.1"
                     /translation="MNWLLSVGDTDILRDAVSPMAVFWHRMRGGRTLVAGSLALTALL
                     GFFIILAVLGPDRTGWVWAQRDWMAIAASVIFAGWAGYNGWLLFQFMPSRNDRVVLRL
                     RELVRQGIDPLEEQIRALSDDQLRAKTAEFKQRLAAGESLDDIRPEAYACVREASRRA
                     RNHRQFECQLIGAKVLEDCNVAEMRTGEGKTIVCYAANYLKVLQGLKVHMVTVNDYLV
                     KRDAEFCRPIFDLLGVTVGYITSDMETYGAEAEVRREAYNCDITYGTNSEFGFDYLRD
                     NMKHSARDQMQGRQDYVVVDEVDSILIDEARTPLIISGPAHDDVSNYRVADNIARMLI
                     AKQQAANRETARRLNDLEQNPPPEARNNPKFYDGIKKFRADPLWLSSEEAEAIGHMQY
                     FVVELDRKSAHMTEHGAKAAQAELAIGTFYDTKNMNWPHYIDNALRAHLAYQKDKEYV
                     AVDDQIIIVDEFTGRMMHGRQWSDGLHQAVEAKEKVTIKQETQTLATITLQNLFKMYR
                     QLAGMTGTAMTEADEFMKIYKLEVIAIPTNQPVRRIDYNDKIYKTRGSKLDALVEEIR
                     AYSQKGYPADAYSLLDMLKMARRTLVSLRDANGNGQAPEEARVGQAASLPGLERIDEQ
                     IRIADEAIRAFGNGEGDEAALYAGLEEGYRKLMGQNVGGRPVLVGTTSVEASEELSQL
                     LSRRYGIEHEVLNARHHAREAEIVAKAGQQHEVVRGKKKEMHGNVTIATNMAGRGTDI
                     VLGPGVAAIGGLHVVGTERHESRRIDNQLRGRCGRQGDPGSSRFFLSMEDELLRLFMG
                     EWMLKMLNMLGFEEGMAIDDRRISKGIERAQKKVEERNFGIRKNLLEYDEVMDFQRKS
                     FYSMRQQVVEGRGLSEMIWRMIDESVADAIDRFYDPQYPAICAAEWVGQNLNVPIEAD
                     KLDPGDGVEELSKTVRDLAAYEVRGTIQRTFGEYVDTDVDPEEWDVRGLASWAAQFGL
                     NLTQNQIRKAQPDELLEQLIEAAQKRIEEADLSGLEPYCDSQFAKARLVRWAQEKFGV
                     EVPLEDLATANREDAEQIFREKMRAAYKIREIEYPVDAVLQFALHAGGQNVNEVYTRI
                     ANWVNRKYGLNWTYEHLAGKSPQQIFQELRAINEDYLTNGKLDREIDQALAKYSGDAL
                     VQWAQQRFGSAVDSQPIEAGDAASPESAARVREQLQHCAYEMLRWELTQLERHVLLST
                     FDSLWKDHMYSMDLLRHGIGLRGYAERDPKIEYKREGTRMFNEMMVNIRERVTDLIFK
                     VQIAMRPDEGAGARVAAAGGGLAYSNLQTTKADATGAGFASAAADQDAAMQKQGEAAK
                     PQTIRRETPRVGRNEPCPCGSGKKYKQCCGKAR"
     gene            complement(48810..49376)
                     /gene="efp"
                     /locus_tag="PLU99_00185"
     CDS             complement(48810..49376)
                     /gene="efp"
                     /locus_tag="PLU99_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO15499.1"
                     /translation="MIKASELRKGKLVSYNNDLYTVHSMQHVAKGNWRSYIQARLKNI
                     KTGQVIDARFNTDEVLETPFVENKPFEYLYRDGDDFVLMDQGNYDQIHVSKDVMGDAE
                     LYLKGNEVVVCTIIDDKIVGVELPNVVELAVVDTTPPIKGATVTNQNKDAELETGLRV
                     KVPPFIENGEVLRIDTRTGEYIERAKGK"
     assembly_gap    50139..50238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<50239..51071)
                     /locus_tag="PLU99_00190"
     CDS             complement(<50239..51071)
                     /locus_tag="PLU99_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOO15500.1"
                     /translation="MGLLKRVVSEFFKDDAMTHAAALAYYGALSLAPLVLILLWIASF
                     VGPTAEKTLVDQAVTAMGEQAGSSIQQVIGGARARPTLANISGAVGLGVLLFSAASVF
                     VQLQHALNRIWDVKPRPDAGIWNTVRKRLLSFAVVLLMGLLLMASLVLGFVLSAGLKA
                     FGESATLLRLLNEAVIFVVLTAFLALVFKYLPDVKISWRDVWLGAAVTALLLSVGKLL
                     IGLYLGRSGVASGYGAAGSIVLLLVWAYYSGIILLFGAEFTQVWAGRSGRRIVPDEHA
                     Q"
BASE COUNT         9365 a        16163 c        16219 g         9374 t
ORIGIN      
        1 gatgagaagc acgcgcgtca tgaacattta tcggcacccg aaacccgacg cgccaaatga
       61 gaacccgccc atggcaaaac gcacgcggcc agcaccgccc gcaaacacaa acggaaacgc
      121 ctggcaggaa ctgggcttac agcccgacct gctcgaaggc gtgcgcctgg ccaagttcgg
      181 cgaaccgact gaaatccagc gtgagctgat cccggtcatc ctcgccgggc gcgactgcct
      241 ggggcaggcc aagaccggca ccggcaagac cgccgctttc gccttgccca tactgcaact
      301 caccaccgcc ggcgagccca cccaggccct ggtgctggtg ccgacacgcg agctggccgc
      361 ccaggtcgac agccacattc gccagctcgg cgtgcgccgg ccgctgaaga ccgcgctgct
      421 ctacggcgga cgccgcatcg cgcagcaggt caatcagctc aaacgcgccc cggagatcgt
      481 cataggcacg cccggccgca tcctggatct gatgcagcgc cgcgaactga acatctccga
      541 tgtgcgtttt atcgtgctgg acgaagtgga tcgcatgctg gacatcggct tccgcgatga
      601 cattcgccgc attctcaaag cgattaaatc agaacaccag accatcttcg tgtctgccac
      661 gattgatgac gaaattcgca cactggcgcg tcccttcacg cgcgacgcag tggagattga
      721 tgtctcgcgc gacaccctaa ctgtagagaa catcaatcag gggttcgtca gtgtcgatgc
      781 acaccacaag tttgagacgc tacttggatt tttgaaacac gaaaagccgc gtctggcaat
      841 tgtctttacc aataccaaac atgccgcgcg acgtgtggcc gaacgtctca agcacgccgg
      901 tgtcaactgc aaagagatac acggcgacct gatgcagtcg cgccgcgaac gggtgatgaa
      961 gagcttccgc gccgcccaga ttcaggtgct ggttgccacg gatctggcct cgcgcggcct
     1021 ggacgtgatg gaagtctccc acatagtcaa ctatgacatc ccggaagacc cggccggcta
     1081 cgtccaccgc gtcggccgca cggcgcgcat gggcaagagc gggtatgcgg taagcttcgt
     1141 cacgcccgat cagggcaagc tgctgactga ggtcgaaaag ctgatcaatc gcgagctgcc
     1201 gcagttcgac gaacactggg tcatgaagcc gcgccgggcc ccggagaccg ccgccgcagg
     1261 cgcggcgccc gaagcggcgt ccggtccgca gccggcgggc gattcagccg ctgcggcagg
     1321 ccccgtgccc acccgctaca gggaggcaat ccgccgctgc gcaattctcg aatcccatgg
     1381 gctcaaaccg ctgcggcgca cgctcggcag ccgcttccgc tcaacccgcc gtcgccgcta
     1441 gtacgcggat tcctcgccac acatcgatgc cgtaagatac gctggccttc agcggttatt
     1501 gccaacgccg agcgacagga gcggcatgcc agagtaccgg gcagcattct gcggtggggc
     1561 cagaacggat gccggttcga ccggccttcg ctctgggcgg ggcttcgttt accggcagga
     1621 ctccgtggcg gcacgccccg gctctgctag cctgaagatc gcggatcgca tcctgctgct
     1681 cgcgctcagt ctcgtgctgc tgctgcctgg cacatgggtc agtctgaccg accgcgatga
     1741 ggggtactat ggccaggtct gccgcgaaat gcttgccggc ggagactggc tcgtaccgca
     1801 gtacttaggg caaccgtggc tgtttaaacc gccgctgctc tattgggcag tgagcggcgc
     1861 gttcacgatc ttcggcttgc aggaatgggc cgctcgcctc gtctctgtgc tcgcgctgac
     1921 aggcgccgtc cagttcctcg ccgcgctcgc ggctgacatg tacaaccgcc gcaccgcgct
     1981 gattgcctgt gcctgctttc tcacggccgg cctcccgctc atcgtcgggc gcatgctgct
     2041 gaccgatccg ctgctgctgc tgtggatgat ggccgcggtc tggctgctgt ggcgcatcgc
     2101 gacccgctcg gccggcgcgg cgctgtcggc cggattctgg ctttcgctcg ggctgggcgt
     2161 catgaccaag ggccccacga ttctgctgtt catgggagct ttcgggctgg cgctcctgtt
     2221 gcaaccgggc cggcgaaaat ggatcgtcag cgggcgcttc tggatgatgt cgccgctgat
     2281 cgcgttagcc gctccgtggt tcatctacat tgcgcagcgg cacggcgatc tgttcacgca
     2341 acgctttctg tttgcggaga tcgtcctgag aatggtcgag cccagttgcg ggcacgtggg
     2401 cccgccgggc tattacctgc tgctgagcct ggctggcctg ctgccctgga cggcgctggt
     2461 acccggcgca gttgccgaaa cgtggggaaa caggcgcgcc gatcgcgcag cgccgttact
     2521 gctggtctgg tgcggactgc cctggctgat actggagttg atggggacca agctgccgca
     2581 ctacgtgttg ccctgctatg tgccgctggc gatcatgctc gggcggatgt gggatgccgg
     2641 cgccgggcgc gagcttctgc ctgggcagcg tgccgtgctg cgcctctggg cggctgtgcc
     2701 gatcgtaatc ggcttgggca tactcaccgc ggccgtgttc taccacagcg cggcttgggc
     2761 ccggccggcc ggggcggcag ggctggttct ggtcgcgggg ttttggctgg cagcccgggc
     2821 ggcatgcaga cgcccgcttg cggcgtaccg tcgggcggtg gcgagcatgg cgctattctc
     2881 gattgtggcc ggcgcgtggc ttctgcccag cctcgaaccg catcgcctga gccgagtcgt
     2941 tgcggaaaag gccaacggct gtaattccgg catgccggtc atcgtcggcg ggtacgaaga
     3001 gccgtcgctc tttttctacc tggcccagcc gccgcaggtg ctgaaagggc gcgacctggc
     3061 cgacggtctg gccgatctga agcggcccgt cgtcgcgatc ctgggcgcaa gcgagctgac
     3121 cgcggcgggg ctggctgaat cagccgcccg tgagggatgg acgtccgtca cgggcttcaa
     3181 ttacagctat ggccgtacgg agacaatttg ggtcggccac ctcgacccgc gtgagcggag
     3241 cccgggggcg tcgcgcccga ccgtctcagg agggtgacgg atcggcttgg ctgaaggggg
     3301 cgacgcctcc ttaaccagcc tatgctttca ctcagccccc gaatggcacg ctacgtgcct
     3361 atgctacgtg ctgcttattg agcggaccaa gagagaatag aaatcaccga ggcctgtaag
     3421 cttgtcgcaa ctatttagct ctcagttttt tacgctcttc gccatcatag cgttgggcgt
     3481 cacgctcggg tcgttccgcc tcggcggaat cgcgctcggc tcggcgggtg tctttttcgt
     3541 ggccctggtt ttcggccact tcaaatgcac cgtatcgcac gagctgaccg aactgggact
     3601 ggtgctgttc gtttacgccg tcggcctgca ggccggccca cgctttttca gcgtgctgcg
     3661 ccgccgcggg gccgctttcc tggcggtggg gttggggtca acggcggtcg gcgcgctcgc
     3721 ggccataggc ttggcgaagt ggttcgggct ctcggcgaac ttggccgccg gcttgtactc
     3781 gggcgccacg acctgcacac cgggattggc ggcggcgctc gaggcggtcc gccgcgtgac
     3841 cggtgacccg ggcagcgccg agaccatcga agccgtcaac gcaacctcgg tcggctacgg
     3901 tgccgcttac cccttcagcg tcgtctgcgt cgtgctgctg gtgcaacttc tgccgcggct
     3961 tctgcggacg ccggccaaga ccgcggccgc cgcctttcgc gccgaagaag aaacccacac
     4021 gccgcccttg gatgaatgcg cgtttcgtgt caccaaccgc aactgcgccg ggcggacggt
     4081 cgaggagcta caggctttgc acctgagtaa tgccgtcctg tgccgcataa agcacgaggg
     4141 cgagatttat cccgtgaagc cggagacgac gctccacctg ggcgacaccg tactggcggt
     4201 gggtacgccg gaggagttgg ccaagcttga ggctgttctg ggcgacgtag tcgtggaacc
     4261 gatgcaggac cccagcggta aagtcaccag cgaactgctg ctggtctcgc gcaaagacgt
     4321 ggtcggcaaa tcgttccgcg aactgtgcat ttgggagcgc tttggcgtcg tcgtcacccg
     4381 cctgcgccgt gagaacgtgg aattgacacc gcgcggtgac gtgcgtctgg aaccgggcga
     4441 cgtgctgcgc gtggtcggca cgcgacgcga cattcgcgcg ctggccgatg ccctgggacg
     4501 cgaagagcgg cgcctggccg aaaccagctt tgttcccttt gcggccggaa tcgcgttagg
     4561 ggcggcggtt gggcagattc cgatcacgct gccgggcggg ctggaagtgc ggctgggcat
     4621 gggcggcggt gcattcctgg tagccctgct gctgggtcac ctgggaacga tcggaccggt
     4681 gcgggtttat gtgcccaacg cggcgaagta cttcgcacgc gagttgggac tggtaatctt
     4741 cctggccggc gcgggagccg gggcgggaca gcgcttcgtg cccatcctgc gcgaagccgg
     4801 cggccaactc atcctggccg gggcgctggt gactctggcg acggtcgtaa cggccctttt
     4861 cataatttat gcgctctgcc gctggaacct gctatacggc gccggggcat tgtcggcctg
     4921 catgacgaat cccccaggct tggcggccgc gacagccctc accgactcgg acgccgcgcc
     4981 ggtggggttc gcgtcggtgt acccggtagc gctgatcgca aaaattctgt tcgcgccctt
     5041 ggtgtatctg ctgctgcgga tgtagcgtaa ttggggcgcg ttgccgttcg gctacgcgtg
     5101 cgccagtgtc aaaccgaaag ccgttgtctg ccgtgcaggc ggcggctcat ctcgagctgg
     5161 tttctcagca tctgctctcg tccggctaaa gcggacgctc ctgaatagct tagacgggac
     5221 caccagtggg gtacgacacc acgcttgcgc ccggtggtgc gcttgcgacc cttgccgtat
     5281 actcgccgta ctttgcgtgc agccgttgcg gcgggttcgc ccggccggtc ggcctccgcc
     5341 gtaaacggca gaatcagagc gagcagcaac aggaaccagc ttagatgatc aaccgcctgc
     5401 aacaacttgc cgcgttggga caatccgtat ggctggataa ccttagccgt gacctgctgg
     5461 attccggccg gctggcggag ctggtggatg agggcctggc gggggttacg tccaatccga
     5521 ccatcttcca caaggccatc gccggcagta agacctatcg cccggaaatc cagaggttgg
     5581 cccgcgccgg caagaccgcg atggaaatct acgaggagct cgtcatccgc gacgtgcgcg
     5641 acgccgcgaa tgtgctgcgg ccggtgtaca acgagacgca tgggcgcgac ggcttcgtca
     5701 gcctggaagt caacccgaag ctggcttacg acacggaggg cacaattgcc gaggcacgcc
     5761 gcctgtttcg ggcggtcgac cggccgaacc tgatgatcaa agtgccggcg acggaagaag
     5821 gcatgccggc catcgaggcg ttgatcgccg aaggcgtcaa cgttaacgcg acgctgattt
     5881 tcgccatccc gatgtacgag cgcgtcatgc aggcgtacat ccaggggctg cggcggttga
     5941 taccgacggg caagccgctg gggttggtgt cgtcggtggc ctcgttcttc gttagccgtg
     6001 tggacacatt gaccgacgag gttttgcagc accgcatcac gcacgggcgc gaggagcacc
     6061 tgacgccgct cttcgggcag gcggcggttg ccaacgccaa gctcgcttat gcgcggttca
     6121 agcaggtctt catcggcgag ccgttcgcgg acctgcgcgc ccgcggcgcc cgcccgcagc
     6181 gcgcgttgtg ggcgagtacg agcacgaaga acccgtccta cccggacctg aagtatttca
     6241 acccgctcat cggcatgtac acggtcaaca cgctgcccat gcagaccttg gaagcggtgc
     6301 tcgaccacgg gctggcggcg cagacgatcg aggatgggtt ggcacactct gaggcggtca
     6361 tgcgcgaact ggccggcgtg caggttgaaa tggagtggat aaccggctgt ctgctgaaac
     6421 agggcgtcga ggcgttcaca gagtccttcg accaactcat cgccgagatt gaagcgcagc
     6481 gcgccgcagc cctctcccgc cagccggcat agcagtcttc gggcagcgca taaaaagccc
     6541 ggccgttttt ggccgggctt gttgtttttc gaccggcgaa agccggccgc atgccatgtt
     6601 gtagagctgc gaggtctact cgatcgcgac cgtcgcgccg gcttcctcga gctgcttggc
     6661 ccacttggtg gcctcgtcct tggaaacgcc ctcgagcaag ggcttcgggg cggtgtccac
     6721 gagttccttg gcttccttca agcccaggtc gctgcgggcc gcgcggacca ccttgatcac
     6781 ttgcagcttc ttgtcgccgg ctgacttgat gaccaggttg aattcggtct tctcttcggc
     6841 cgcgggcgcg ccaggtgcgc cggcgccgcc gaccgccgga ccagccatca ctaccgcgcc
     6901 gcccgccgcc ggctcgatgc cgtaggcttc cttgagataa tcagtcaggt cgcgggcctg
     6961 gaccagcgtc aggccggcga tacgatcgcc gagttccttg atcgcggcgt caaaagtacg
     7021 ggcaggtgct tctgccatgt cactaactcc aaacttcaat ttccagtcgc cccgatagct
     7081 gaagccccgt tggcttcatt taatcccgtg agggagcagt cgggcacgaa agaattcgca
     7141 aatctaaact actcggccgg cgccggggcc ggttcggccg cggccggggc ggcttcagcc
     7201 gccggcggcg gggccttttc aagcttttcg atcagactct tcaggcaacc ggcgatattg
     7261 cggccgccgg ccagcatggc cgaggccaag ttggcgccgg gcgaacggac gcagctcgcc
     7321 aagcgggcct gcatgtcgcg cttggtcggc attttggaca ggccggcgac cgccgcctca
     7381 tccagaaact cgccttcgag cagggcaccg cgcaggcgca tgcctttcag ccgcggcagc
     7441 cattgatcaa gaaacttggc cgcctcgacc gcggagtcgc cgccggtaat cagggccgcc
     7501 ggcccgtcga gcttgtcggc caggcggcta agcggtccat cgcccacggc gcggcgcagc
     7561 agggcgttgc gcaccacttc caggcgcagc tttttctcgc gcagctcgcg gcggaactgg
     7621 ttggtcgtga tgccgtcgat gccgatcatc tccacccaga tcgcgttgtc cgtctggctg
     7681 tagcgcgacc gcagctcctt cgtaatcagg tctttgacaa atttgctcat atgactactc
     7741 cagtccgctg ccggtggacg tatcaaccag gaccgacggg gtcatcgtgc cgctgagggc
     7801 cagtttcttg atgaactgtc ccttggcggt gggcggcttc agtcgccgaa tgtgatccat
     7861 gaaagcgacc acgttctctt tgaggtcggc ttcgctgaag ctcagcttgc cgacgacggc
     7921 gtgcagattg ccgcccttgt cgttgcggaa ttcgaccttg ccggccgcat actcgcgaac
     7981 ggcggtggcg atgtccggcg tgacggtgcc gctcttgggc gagggcattt tgccttgcgg
     8041 gcccagcacg cggcccagct tgccgacctt gcccatcatg gaggggtggg cgatggccac
     8101 gtcgaaatcc atccagccct tttggacctt gtcgatcagc tcgtcgccgc cggcctcgat
     8161 ggcgccggcc ttcttggcgg cctcggcatc cgcgtcgccg cagaaggcga ttaccttctt
     8221 ggtcgcgccg atgcctttcg gcaggctgac cgatccgcgg atggcctgct cagcctggcg
     8281 cgaatcgatt cccaggtggg cgaccaggtc gaccgtctgg tcgaacttgg tcttgctgaa
     8341 agttttgagg gttttgattg catcttcgag aggaaccggc ttggcgggcc gcttcttctc
     8401 gtcctgtctg taacgcttgc cgcgaagttg aggcatcgct aaaagctccc acaaaagttc
     8461 cgggcccgtg gtcaggccca aacgtgaaaa ggtgagaagg tgagcaggtg atcaggtgat
     8521 caccctatca cccggccttt acgtcaatct attacctcta tgcccatcga tcgcgcggtg
     8581 cccgcgatga tccgctcggc cgcgtccaga tcgaaggcgt tcaggtcctt cagcttggtt
     8641 tcggcgatct tgcggacttc gtcgcgggtg acgcggccga ccttggtctt gttcggttcg
     8701 ccggagcctt tttcgacgat ggcggcgcct tcggcggtcg gcttggcggc cttgatcagc
     8761 agggccgcgg ccggggggct cttgacgacg aactcgaagg ttttgtcctt gaagacggtg
     8821 atctcgaccg ggcaggtcat gccgtccatg tgcttggtgc gttcgttgaa ctgggagacg
     8881 aactgcccga tgttgacccc gtgctggccc aaagccgggc cgaccggcgg cgcgggggtg
     8941 gctttcccgc ccggcgcttg caacttgatt ttcgcgacta cggccttggc cataacagtt
     9001 ccctgatgtt tggaccgcta ccgccgcccc tgaggcggcg aaagatcagg caatatagca
     9061 cgaaacccgg gtacctttca atggggcgaa atttcgtcca gcggggatgg cggcagcgag
     9121 ccggcgaggg ggtggcgcgg ggccgcgggg acggctgtgc gggagaagag gcgcttggcg
     9181 gtagttcggg gcgacacgcc agaggggggc actacacgga tgcgagattt ttggcgatac
     9241 tccggggcga cacgccccgg ctctgctctg tcggggcgag cggcagcgcg ggctatgtaa
     9301 actccgctgg catcagccct tgcgaaatat cctgcagcgc cgcgaaatcg cagatccgga
     9361 tttgctgcgc gtcgggcgtt tcgatcaagc ccgcttcggc caagcggcgc agcgtgcgcg
     9421 acagggtctc gctggttaga ttcaaatgac tggcaaggtc ctttttcagc gtcggcagcg
     9481 tgaaggccgc ccgtccgccg ctggggtcgg ttcgcaacag gtagcccgcc acccgcgccg
     9541 tcgcgtcacg cagaacgatg tcttccaaca gcccaacgag ctgccgcacc cagaatccca
     9601 tcccctccac gagctgcaag cacacttcgt ggctgctgcg caacaggcgg cgaaaatcat
     9661 cggcttggac gaaggcgcag accgtgtcct cgagcgcctc ggcgtgggcc ggacagctaa
     9721 agttgctcat caccgcgacc tcggcgaacg tcatgccggg ttcggcgaag tggagcacgt
     9781 gatccttgcc ggtcggggcg atcttgtaca cgcgcaccag cccctgcccg acgcagtaca
     9841 gccccgggca ttcctccccc tggcgaaaaa taatccggcc tttttcaaaa cgcaccacgc
     9901 gcgcccggcc ggccaacagc tccagcgaat cctcgctcaa gctcttaaac agccggcact
     9961 tacgaaaaat atctcttacg tcttgcgcca cgacccgcag acctcagcaa aaaaatttct
    10021 acggcttgat ccagatcaag gcccggaatt caggattctc cgacacttgg agcggtaggg
    10081 caacctgtgt caatgacgag aacctgtggg accggctgtt aaccggccac tgtcgtggga
    10141 ccggtttgac cggccagtca aaacgaggac gagccatgct gcgagaagtg gtcaccattg
    10201 atgaggaact gtgcgacggc tgcgggctat gcgttccagc ttgcgccgag ggcgccctgc
    10261 ggatcatcga cggaaaggct cgcctggtcg ctgatcgcct ctgcgacggc atgggcgcct
    10321 gcctcggtca ttgcccgcgc ggcgcaattc ggatcgagca ccgcgaggcc gaagagttcg
    10381 acgaagccgc cgtcgtcgcg gccgctcacg cgccgcaaac gcgaacggcc gaacccgcca
    10441 ccgcccgcgc ggcggcttgt cccgccccga gccattcgca cgccggcggc tgcccgggaa
    10501 tgcgtctcaa tgtttgggac gtcgcgtccg gaaacggcca cgcagcacgt cccgcggcag
    10561 cttcgcggcc ggaaaaagcc gcggcgcctt cggccctgac gcattggccc gttcaattac
    10621 ggctcgtacc gccaacggcg ccgatgctgc gcggagcacg cctgctggtc gctgcggatt
    10681 gcgtcccgtt cgcatacgcc ggctttcacg ccgatctgct ggcgggtcgc gcggtcgtcg
    10741 tgggttgccc caaactagat gaccggcagg agcagatcgc gcgccttagc gctatcatcg
    10801 cacaaaacga cttagcagaa atcacggtcg cccacatgca ggttccgtgt tgcactggaa
    10861 tcctgcacgc cgtgctcgaa gcgcggcagc gggccggcag cagcgtgccg gtcgtcagtg
    10921 tggtgattgg tattcacggt gatattttgg agcggcgcga agttgtcgcc gctggcgcgg
    10981 agttttagtt acgtccggtc ggccggacag caaggagcat tttggatgtt ttgtaatcag
    11041 tgtgagcaga cgcgcggatc gggatgcgtt gaggtcggag tgtgcggcaa gaatgccgac
    11101 gtacagtcgc tgcaggaaat cctgctctat ggcctgaagg gaatggcggc ttatgcccac
    11161 cacgcccgcc ggctcggagc cacggacgag tcggtcagcg cctttatcga agaggccctc
    11221 ttcgccacga tgaccaacgt caattttgac gtgcccacgc tgttcgagat ggcgcttgaa
    11281 tgcggccgca agaatctgcg cgtcatggag atgctggatg aagcccacgt gcagcggttc
    11341 ggcagccctt cgcccgccac agtctatgaa ggcaccaagg ccggcccggg cattctcgtg
    11401 accgggcacg acctgctcga ccttgaagac ctgctgaagc agaccgaggg tacgggcatc
    11461 aacgtctata cgcacggcga gatgctgccg gcccacatgt atcccggcct gcgaaagtac
    11521 aagcacctgg ccggcaatta cggcggcgcc tggcaacggc agaaggtaga attcgcggag
    11581 ttcagcggcc ccatcgtcgc caccaccaac tgcgtgctca tcccgcgcga ggagtacgcc
    11641 gaccggctct tcaccacccg cgtcacagcc gtcccgggcg ccaagcgcct gacaagcaat
    11701 gatttctcag cggtcatcga gtgcgccaag aagtgcgccc cgctgccgga agagcgcgtc
    11761 cgcgaatcga ccgtcggctt ccatcagtcg gtgatcctcg gcctggcgga caagatcgtc
    11821 gccgccgtca aggccgggaa gatcagccac ttcttcctgg tcggtggctg cgacggctat
    11881 gaaggcgcgc gaaactactt ctccgacttc gcccaagcca ccccgaagga ttcactcatc
    11941 ctgacactcg gctgcggcaa gttccgcatc cgtgataacg attacggcac gctcgacctg
    12001 cccggcgtcg gcccgattcc gcgtctgttg gacatgggcc agtgcaacaa cgcctacggc
    12061 gccatccagg tcgcggtcgc gctcgccaag gccttcgact gcggcgttaa cgacctgccg
    12121 ctgacgttgg cgatctcctg gttcgagcag aaagccgtcg cgatcttcct caccttgctg
    12181 gcgctggacg taaaaggcgt ccgcctcgga cccaacccgc ccgccttcgt ctcatcgaac
    12241 gtgtttagaa ttctgcagga caaatacaac ctgcggctga cgaacggcca cgccgccgcg
    12301 gacgtgcagg ccgcactggc acataaatag gaatgtggcc aggccggagg tgaaacatgg
    12361 ccgggcagtt gttcgaacgc cacgttgagc tgtacgacgc cctgattgat tggccgaagc
    12421 gtctggccaa cgagggtccg ttcttgcggg ccgtgttcga ccggtgcggc gcgcgacgag
    12481 ttctggacgc cgcgtgcggc accggccagc acgccgccat gtttcactcc tggggcttgg
    12541 aagtggaggg cgcagacgtc agcccggcca tgatcgcgcg ttgccggcag cagtttggcg
    12601 agcccggcac actgcgctgg acggtgcgcg gcttcgagga gccggcgccg gcggcggtcg
    12661 acgccgtggt gtgcctgggc aactcgctgg cgctggcggg caatcgcggt ggcgcgcagc
    12721 gcgccgtggc ggcgatgctg gcgtcgctgc gttcgggcgg cttttgcgtc attcaagtgc
    12781 tgaatctttg gagcctgccg gagggtccgt gcatctggca gaagttccgg cgagctcgag
    12841 tggacggacg cgagcatttg cttgcaaagg gcgtccatcg gcacgccggc gcaggcttcg
    12901 tcgagttcct cgctctcccg gtcggggctg acgaggaacc aagttacgaa accgtgcggt
    12961 taaccggcct gacccgcgag gacctggcgg cttttgccag cgccggcggt gcgcggcagg
    13021 tggagttttt cggcaactac gcgcgtgatg cgtatgagcc gcagcgttcc gtggacttga
    13081 tagccgtggt cgaaaaggcg tgatgcgcga ttctggccaa caagccgagc acagtcgaac
    13141 gcggcaggcg gcggccggcc cgatgatcga ccgcaagctg cgcaaggact tgcgcacgct
    13201 ggcgcttttt atcgagttgt actgtcgcaa acgccacggc ccgatgcctc gcgccgccgt
    13261 acgcctgcgc acgctcgacg tggccggggt gcatggccgc ccgctggagc tgtgccccga
    13321 ctgcgccaag ctggcggccc acgcgttcgc caagcgcatg caatgcccgc tggaccccaa
    13381 gccggcctgt aaacattgcc cccagcactg ctacgccgac cagtaccgcc ggcaaatacg
    13441 cgaggttatg cggtacccag ggcgccggct ggttctttcc ggccggcttg attatttgct
    13501 gcacctgctc ttttagcagg tggcgccaaa ggcggtatgt tgcgggccca cgcgcccgcg
    13561 ggtacttttc gcaaccgggc cgaaggtgag tcgcgtgcga aagctgaatg ccgtaaagtt
    13621 ggcggcgttt gccggtctgg gactgttagg cgtgggcgcc ggcgtcgggg cgtatacctt
    13681 tatttacgct gaaggcgctt cgtacttgac cgatgacgcg gccgcgtgcg ccaactgcca
    13741 cgtaatgcag gagcagtacc gcgcctggaa ccgctccagc catcacgcgg tcgcgacctg
    13801 caacgactgt cacacgccac acgattttct aggcaaatac ctggtgaagg cccgcaacgg
    13861 ctatcaccac tcgctggcct tcaccaccgg ctggttccac gagccaatcc agataacgcc
    13921 gcagaatctg gcggtaaccg aagccgcctg ccgccgctgc catcaggaca tcgtgcaggc
    13981 catcgacacc agccagcccg gccagccgcc gctgtcgtgt acgcgctgcc acccaaacgt
    14041 aggccacctg cattgaaaga gaaccaattg tcaacattgg ctgcgtacat gctcagtgtc
    14101 ttctctatgc ctcgttgtct gtgtggttta actctctgga atgtggaatt gagacatggc
    14161 aaaccccgtt gaaccgccga aagacgtgtc gtacaccaac ccgccgcggc gcggccgaac
    14221 cctattgata gccgccgccc tggcgttggt cgcggccgcg acgttcggag tgacggccct
    14281 gctggtcaac atcttccagc acaagcagga agcgcgcaac cccttcttcc gcgtggtcga
    14341 gctggacgac aacacggtcg atccgcaaat ctggggcaag aacttcccgc aacagtatga
    14401 cggctatgcg cgcacggtag acatgcagcg caccaggcac ggcggcagtg aggctttgcc
    14461 gcacgagcca tccgaaaagg acccgcgcac caaagtcgcg cagtccaaaa tcgaactcga
    14521 tccgcgcctc aagaccatgt gggccggcta cgcgttcgcc aaggactttc gcgaggagcg
    14581 cggccacgcc tacatgctcg aagaccagac ttacactcag cgcgtgcttg agttcgcgca
    14641 gcccggcacg tgcctgaatt gtcacgcctc aacctacgtg gcctacaagc aacttggcga
    14701 cggcgacatc atggccggct ttgacaaaat gaaccaaatg ccctacgccg aggcccgcca
    14761 gcttgtagat cacccaatca gttgcctcga ctgccacgat ccgcagacca tgcaacttcg
    14821 cgtcacccgc ccggccttca ttgagggcat tcgtgtggtc aaggcggccg agggcgtgcc
    14881 ggacttcgac gtcaaccgcg acgccacgcg ccaggagatg cgcaccttcg tctgcgcgca
    14941 atgtcacgtc gagtattact tcaagggcaa agagaagcgc ctcacgtacc cgtggttcaa
    15001 gggccgcaag gcggatgaaa tcctcgcgta ctacgatgaa gtcggattca cggattggac
    15061 gcacgccgag accggcgccc cgatgctaaa ggctcagcac cccgaattcg agatgtggag
    15121 ccagggcatt cacgcccgcg ccggcgtaac ctgcgctgat tgccacatgc cctacatgcg
    15181 cgtaggcgcc atgaaggtca gcgaccacca cgtgcgcagt ccgctgctga acatcgccaa
    15241 cgcctgccag acctgccatc gtgtaccgga gcaggaactg ctcgcccgag ccgagaccat
    15301 ccaatcccgc atgttgttcg cgcgtgactt ggccctcgac gccctcatgg agctcattga
    15361 cgacatcaag gacgcgcgcc aggccggcgc cacggacgaa gagttgaagg ccccgcgcga
    15421 cttccagcgc aaagcctcgt tcctggtcga cttcgtcgaa gccgagaatt ccgccggctt
    15481 ccacgccgat caggaagccg tgcgcgttct catgcagtcg ctggaatact cgcgccaagg
    15541 ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttatca ctctggcgct
    15661 acaaagtctc gtccactcgc cttcaccgtc agcaccggac acggcgcgtg ttgcacaatg
    15721 cgctcggccg tactgccgat cactatatgt gtcaaaccgg tgcgtccatg tgtactgacc
    15781 actatgagtt ctatctggtg ggctgccgcg tattcgcaaa ccgccgacca ggcattgccg
    15841 aacagcactt cctgctgaac atcgtcggtg gcctgcagac gctccgttat gatccgctga
    15901 agccccttgc ggcaggcttg aatcgtttcg gggtcagcgg ccgtcaccgg cagcgtcgcc
    15961 ggcaaggcga ctgagaagtc ggcggtcatg atcggaggga tcacgtgcac cacgtgcagt
    16021 cgcgcgccga agtgccgcgc cagggcataa gcatagcgcc ccgccttaag tgacagctcc
    16081 gagaaatcgg tcggccagag aatgcgttcg agtgtgaagt ccatagcgca cctcgcctga
    16141 attgtattaa acagagccgg accgcggcgg tccggatcat cagctcgcga tggggccggc
    16201 gtgtgccggg ctcagccttg atccaccttg gccatcgcac gcgacttttg gcgcaaggcg
    16261 gcgctgacca attcagccac ccgccggcca tccacgcgcc cgcgcagatc gcgcatcagc
    16321 acgcccattg cgtaacgctg catgtcgcca gcgtcggcga acttcgacga atccagccca
    16381 tccaccgtgc tggaaaccag cgcactggga tcaccttccg caacgtgccc gtaccccagc
    16441 gcgttcatgg ccgcttggac tcgggcggcc gaatcgcaag gcgcgaagtg gttccccagc
    16501 acgtactcca ggacgtccag cagcccctcg cgcgtcagcc gccggttggc ataggcctcg
    16561 aacgccgcat atatgtctgc gtcgctcaag tcgttcacgt ccagcccggc gcggcgccaa
    16621 gccttcagcc gctgtactaa aaccacggcc gccagcttcg catcaacctt gagatcgcgc
    16681 accagtctga aaaacagctc cccatgccgg gatgtaagca acggcaccag cgtgtcgccc
    16741 ggcaccccca actcacgtaa ccgctgttcg cggttccaga cgcgctctgg taaaccggcc
    16801 cgtatgcgcg ccacgcgctg cgctgaaatc gccagcggcg gcaggtccgt atcagggtac
    16861 atccgggcgg cccccggcaa cacgcgttca aagccggtcg tgccgtcctt cagcggctga
    16921 cgcgtgtccg acggcacgcc cagcgtcgcc tcgcgcgccc ggatggcgat ttcattacag
    16981 gctgtttccg tgtcttcttc gctgccccag accagcagca gtgcatcgtc ggcggccgcc
    17041 cgggtgcgtt tgcgcaaatt ctcccagtcg cgcgccgcca gagcttcagt tgccgtgtct
    17101 gagtgcgtta tgtttggcag tcgcgtcagg caggcgatga cgcgtacgcg gtcggcgaat
    17161 tctttggcga acgtggtgcg ctcctgcgtg accgtattaa gcagcccgcc gaagcgtttc
    17221 agtaccacgc atttgacgcg caagccggcc tcgaccgccg cgcgcaccgg ttcccactgc
    17281 acgcgctcgg tcaggcgtgt gacgtcatgc gccgagtact ggaacgtctc cggagtaacg
    17341 ccgcgccgcc ggagttcctc gcgaatctcg agcaaggcac actgccgatg agcctcgttg
    17401 tagaccagcc gcggaatccg ccaaatctgc ggtacaccct tgatctcaac ccgcgtcccg
    17461 cccgccacgc tgacgttcac gtcctgccgc gccgcgccat agccggtgcg caccttcccc
    17521 gtgctgcggc aaatccgtcg aatcagttgg caaacgtcgg ccgcctcatc cggcgtacac
    17581 atgtccggct cggtcacgac ttcgatcagc ggcatgccca ggcgatcggt caggtagacg
    17641 cgatcgtgcc cgacgtccga aacctcgcgg caggcatcct cttctatcga tagctggcga
    17701 atcccgatcg tccggtcgcc gtacggaagc gccccggtca cgcccacaat gccggtgcgc
    17761 tgaaacccgg ccggaatgga gccgtccagg tattgcttgc gcgctatgtg aagctcgctg
    17821 acgatattca ggtcgaacaa gagacacaat tcgagcgtga tgtcgagtgc gtcgtcgtcg
    17881 atcagaaacg gcggggtgtc gtcaaactcg tacgtgcaaa ccgtactatg gtggatgcga
    17941 taatagatgt ttttgcgggt ctttttttcc atcaaagccg tgccgtcgta ctcgcccaat
    18001 tccgacagcg tcggccgcat atgccgcaaa atctcggtgt cgtaatcgtc gctgtagcgc
    18061 ccggccgggc agcggcagaa gagtttcgtt tcggtcagaa gctgctggtg cacctccagc
    18121 ccgcacttga ggccgatctg cgcatagtca tcgcgcgtca tctcctcgaa cggtttaaac
    18181 cgcaatgaca tgccttacct tctcctgtat agcgcctttc tttcctcggc acctatccta
    18241 gccgttttcc aactttgtgc tatcccatct gttattgcgg cgccgctcaa aaacgctcgt
    18301 tctccatcag ttaacgttcg tacacctgtt cccgagacat tatcggattt cactgctcgc
    18361 acaacggggc aagttatcac actcctggtg ttctcgtaga atagcagcgg tgcgacgtgc
    18421 ctcagctaag ccctgctccg atgccccagc gaccccaaga tcatcccgag gcggttctcc
    18481 aacacgagat cgagtcctac cgtcgtaaac tcgccctgca gatggccggg cggcggctcc
    18541 tcgtcctgct cgcgatcgca gcggtcccgc cgttgctttt cgtcgtggcc gatcattctc
    18601 atccggccgg gctttcgcgc tccactgccc tgctgttcgc ccggtgctgg tgctggttcc
    18661 tcggcctgag ctctctgacc ctgctcgtgc tgactttccg gcgccgcctg aacccgctct
    18721 acgcggcccg cgccctcgaa cgcgccagcg gaatcgatca caactcgatc gtcaatgccg
    18781 tcctcttgca gcgccagcca cgcgccgctt acgccagcac cgcggccgct gagcaggccg
    18841 ccgccgacct gtccgagcac tggccgacgc gcatcggtga atccggcgcg atgcggctgc
    18901 cgctcacgct gatcaccgta gtcttggcgg cctggatcgg ctacgccctg ctcgccagca
    18961 aaccggtctt gccttccgtg gccaggtttt ttggtgcgag ccggcccgcg ccgacggcca
    19021 cgacgctgga acaggtccgc ccgcggcccg gcgaggcggt ccacgccggc caggagctca
    19081 gactcgaatt tgcggctcgc ggcggctcgg ttcggcaagt tacgtttgaa atactcgccg
    19141 ccgacccgga ctccggcgac catgcaatgc gctatgagct caagcccgtg accgaaccgg
    19201 ccgcgaattg gcagctcacg ctcgcgccgc atgaagtgaa ccgcgacatt cactaccgct
    19261 gcaccgccaa tgacgcggtt ctcacaggca ccatcccagt attgcctgtg ccgatgctga
    19321 ccggcctgga gatcgagctc gaaccgccgg cgtacagtgg actgccgatt cagattacgg
    19381 ccaatccgga tttggaggtc ctggccggca cgcgtgccac gttccgcgcc ttcgccaaca
    19441 cggtcgtgga aaacggcacc ttcgttctca ctaacggcca cgaaaccagg acgcggatga
    19501 atgttgaccc cgccgacccg taccgcttca cgttgtcaat ggtgttggtc gaaggtgggc
    19561 agtaccgcat tgagtttcat gaccggctgg gcgccgccgt caaagacccg ccggctcacc
    19621 aaatcacgct ccgcccggac gaagccccca ccgtgaccat cgttgagccg gccgaggccg
    19681 acatgccggg cggcgtggtg gacgtcacgc gccatgcggc gctcgtggcc gtcgccgaag
    19741 acgatgtccg cttagccgag tttgagttgg tgcgggctga tgcgggcgcg ctggcgcgca
    19801 acatactcaa aatggaactc gaagcggacg ggcatcgcgg gcgcgtccgc gtccctacgg
    19861 ccgatctgcc gatgaaaccc ggccagcaga tgcgcgcttg gttcgaagtc cgcgacggcc
    19921 gtacccggtc cgacggcacg cccgcgccgc agaccggccg cagccgcgag atcatcttga
    19981 gtaggccggc cgaggctgtc cgcggccgcc caagtttgcc caagcggcaa accgccagtg
    20041 cccagccgcc gccacccgag gaggccacgc ccgacgagca ttctgcatcg cggccagcag
    20101 gtaacgagcc ggccgccaac cagccgaccg ccggcgcttc gggcgggcaa accggtgatc
    20161 aggccggatc ggccggtccg gatgcaggca agacgggagg gacgggtgcg gaagcgacga
    20221 gttcctcccc ttcgaacgaa ggcgacttcg aacgcgatct ccagcggttt gtcgaagaga
    20281 acgggggcat ggcgcgcgac ctcggacgtc gcataccgaa gccggagccg gaagcagggc
    20341 cggatggcgg tgaatcacag aattccgggc agaccgaacc cgcaggcggc gcgtcgccgg
    20401 agcaaaccga ccaaacgcca cctgcgccct cgcccgcgtc cggccaggac gaaccggcgg
    20461 cccagccccc ccaatctccg cggcccgaag gcacaccgcc cgcgccaacg gccccgcccc
    20521 cgcaacctga cggttctcca tcagcgaatc ggggccagga aaaaccgggg gaggacgaat
    20581 ccaatcaggc tggcggaggt tcgcaacagc agcctcaagc gcccgacccc gcgggcggcg
    20641 cgtcgccgga gcgtgccggc caaacgccac ctgcgccctc acccgcgtcc ggccagggcg
    20701 aaccggcggc ccagcccccg caatttccgc aacctgacgg ttctccttca gcaaataggg
    20761 gccaggaaga accgggggag gacaaatcca atcaggctgg cgagggttcg caacggcagc
    20821 ctcaagcggg cggcgaccaa ccaggtcagg gttccggtca aggcgcgggc gataaggacc
    20881 ggccgccatc tgactctggc tcgacgcagt ccggcggcgg aacaggcagc ggtcacgccg
    20941 actcgccggc gggaaatgga accgtcgcgt taccgcaggc gccccagccg ccgaactggc
    21001 gttcgcggac cggccccctg caagcggaag gcctgaccga aacgctcgac ctattggaat
    21061 ggatggatcg tggcggggaa gttgaggaaa acatgctggt cgatttgggc tggcccgccg
    21121 acaaggcggc cgccttcatc accgagctcc ggcgcctgca cgagcagtac cgccgggcgg
    21181 gtggcctcgg cgagctgcag gccttgcgcg ttcgcctctc actaggcgac ccaaacgtcc
    21241 aggcgggcgg cggcctgtcg agcgccgcgc gaacggcggt tgacctcgcc cgcccgcatg
    21301 aggaccacct gcgccgcatc gccccaccgc cggagcagga agtaccggcc gagctgcgaa
    21361 gtctgctgga tgcgtactac cgtgcgctgg cgcagcgaca gggccgcggt tacgttacag
    21421 atccagcgaa cccatagcgt ttgtccccct cacccgccgc aacgcattcc gcctgccggc
    21481 cgccgcgttt ggggcaggca tccccgtgcc ccagctccgc aggaaatacg ccggacgctg
    21541 caacgcctgg cgaggggaaa accgcgacga attccagtcc tggcgcgcct tgttttggcc
    21601 ggctgagggc gtattatagc gggtctgtcg cgaccggatg gcgcgagccc gtatggcagc
    21661 ctactgtcag gattgatcat gcgttttacg aagtttttcc gtcgttatag ccgcgctttg
    21721 ctgatggttt tcatgtcgtt gctgctggtg acgtggctaa tcactcccgg gagcaattcg
    21781 ggaccctcgc gccgggacga tatcgtgatt ggaacctcca tccacggcga gatcacctcg
    21841 ggcgactggg cccaggcaca ggccgatcag caggcgttat cggctctcga cagctcggtg
    21901 atgcggttcg acccattgga cttctggctg ctgacgaagg aagcgcagcg catgggcatc
    21961 cgcgtcggcc gcgcagaagt gcaggccgcc ctggcgcagt ccgggaaccc gcaagagttg
    22021 cagcgcatcc tggcgggcat ccaaaagtcg agccgccgca gtacggaagg actgtacgac
    22081 ctgatcggcc gctggctcgc cgttcagaaa ctgctgggca tccaggaaga tgcgatcggt
    22141 gaaagtgcgc cgcgcatcga aatggcctat cgcgacgcga cccagaaagc agacgtccag
    22201 gtttcggtca tcgactcgcg ggccttcgtc aatggagtcg ccgagccgac cgaggaggag
    22261 cttcaggcct ttttcgaggc caacaaggac cgtttcccgg ggcataccga cgaccagatc
    22321 gaattcgggt accgcctgcc cgaccgcgtg cgaattgagt acgtgaccat cgatccgaag
    22381 agcctcgagc cgaaaatcca cgcccgccag cgcgaactgg aaaagtactt ccaggagaat
    22441 gcgtggaagt acaccaagac cgttaccccg gagactcagg ccgccggcag cacgccaacg
    22501 ccaaccaggg tccccatgac gtttgaggaa gccaaggacc tggtccgtaa ggatttcatc
    22561 agggagcgcg ccattctgga agcgcaaagt ttgatgaacc aggtccgcga cacagtctac
    22621 gcgccctggc agacgcagcg cccggatgag caaggtttcc ggcccgagcc cgaaggtgcg
    22681 ccagtttcat tcagcgattt gcgtgaacag tttgccaccc gcgccgaggt catttaccag
    22741 cagaccccgc tgatgagcca ggaggagctg cagaagtact tcgatgatcg ccctgacttt
    22801 ttggccaagg agcttgcgga tcagctcggc tcgcccgaac cgatctactc cgaggggatg
    22861 aacagattgt ccgcttccga actggccttc cgcgtgaaag gcctgtacac gccgcagccg
    22921 gaggagcgcc tgccggtgct cagcctgaac gaacccagcc cggttctggt tactcgaaag
    22981 acggacttca gcgttggaat ggggcgtcca tcggaaacgc cttaccagcc ctacatattc
    23041 cgcgtggtgc aggctgagcc gtctgcgccg cccgcgtcgc tggacgaggt gcgcgagcag
    23101 gttgtcgctg acctgaagct gatgaaggcc catgccgccg cgggcgaaca cgcgcggcaa
    23161 ttggccgaag ccgcccggaa cctcgggctg gccgctgcag tggaacaggc cactgagctg
    23221 aagcagcttc tggcggcggc agagccgacc gcccccgcgt tcttgagcga tactcctccg
    23281 gccccctccg cctatgtgac agaactgggc cccgcggcgc cgctgcagcc nnnnnnnnnn
    23341 ccggtgacgc gcgcttctga ccagctcggg caatacctga ctgacagtca gaagttgccg
    23401 cgcgaaattt tcgaggccgt cgaggaagcg ggcccgtcca gtgcatcggc ccacgtggtg
    23461 gtggtgaacg acgtggccaa gctgttcaag tgggcggtgg tcgaggttga gggcctgaaa
    23521 cccgtctacc gcggtgaatt cgaccagcag aggccgcgct tgcacgccat gagctcgcaa
    23581 ctggcggccc aagaggttct gggcggctgg ttgagtcccg agaatattca cctgcgaaac
    23641 aaatttgtcg aaacccgggc gacgcagccg gccgattagc atgggcgcca gttacaattt
    23701 tcgcgagatc gaagtcaagt ggcagcggac ttgggcccgc gagcgtgccg cccgcgcggc
    23761 caaccccggt gagccgggta gcgaacggcc ccagttttac gtgctggact ttttccccta
    23821 tccatcaggg gccgggctgc acgtcggcca ccccttgggc tatatcgcct cggacatcat
    23881 cgcacgtttc atgcgcatgc gcggttataa cgtgctgcac ccgatgggct gggacgcgtt
    23941 cggattgccg gccgaacaat acgccattga aaccggcgtt cacccgcgtg aaaccacgcg
    24001 gcgcaacata gcgacctaca agcgccagct cgagatgatc ggcctggggt acgactggga
    24061 ccgcgagctg gctacctgcg atgaggaata ctaccgctgg acgcagtgga tcttcctgaa
    24121 aatcttcaac tcctggtacg accctgaaag gcgctgggtg gatgccgatg ggcggaccgt
    24181 ggttggagcg gcgcggccca ttgaggaact gcccataccg gcggaagtgc gggcccgcgg
    24241 acagcaggcc atcgaggaat accaggccga gcaccgcctg gcttacctgg ccgaggtgcc
    24301 ggtgaactgg tgtccggcgc tgggcacggt gctgtcgaac gaggaagtca cgaacgaggg
    24361 ccgcagcgag cgtgggaacc atccggtctt ccggcgcccc atgcggcaat ggatgctgcg
    24421 aatcacggag tacgcgcagc gtctgctgga tgatctggag ggtctggact ggcccgagcc
    24481 gatcaagcag atgcagcgca actggatcgg gcgcagcgaa ggggcttatg tcgacttccg
    24541 cctggacgcc cccggcgact tgggacgcca ggtgatacgc gtgttcacca cgcggccgga
    24601 tacgctgttc ggcgccacgt acatggttct ggcgccggaa caccggctgg tgcccatcat
    24661 caccacggac ctgcatcgcg acatcgtaaa cgcgtacgtg gccgaggccc ggcagaaatc
    24721 ggagctggac cgcacggccg agacgaaggt caagaccggc gtgtttaccg gggcatacgc
    24781 aatcaacccc gttaatcggc agcgcgtgcc gatctgggtc gcggactacg tgctgatggg
    24841 gtacggcacg ggctcgatca tgtcggtgcc ggcccacgat gagcgtgatt tcgagttcgc
    24901 gcgcgagttc ggcattccca tccgtgacgt ggtctatccg gcggccgtgg cggccatgca
    24961 ctattttgca caggcggcgc ccgcagaggc gcgggccgag agcgaatggc gcgacgtgct
    25021 ggccgacttc ttggacgccg tggcggccgg cgacgttgcc ccgccgggtt ttgcgcagac
    25081 gttgcagcaa gtgctggcac ggcgcggcgg tcccgggccc gcgccgcgtg cgagcgagct
    25141 attcgccgat ggcgttttga agcaactgcc cggagtgatc gagcgcgagc tggggcatac
    25201 gcgcgtggct tggccagacg tcttcgagaa gttgggattc cgcagcttcg aagacctgcg
    25261 cgactgcttc cagaaaggcg agttctgtac gcgcaccggc gaagcttaca ccggcctggg
    25321 gtttaacgtg aattcgccgg gcgaaacggg cgagcacttc cccgggcaaa gcaacctgaa
    25381 cgggttgcat acgccggagg ccaagcagcg catcaccgcc tggttggaat cgtgcaatct
    25441 gggcgaaggg acagtgcagt acaagctgcg ggactggctc ttctcccggc agcgttattg
    25501 gggcgagccg ttcccgattt tgtaccgccc cgacggcgga atagaggccg tgcccgaagc
    25561 tgagttgccg gtgcgtctgc cggacatgga ggactttcgc ccggaaacca gcgacgaccc
    25621 caattcgcag ccggccagtc cgctggcgcg ggccaaagaa tggcaagagg tggagcgcga
    25681 cggcgtcaag taccggcgcg agctgaacac catgccgcaa tgggcaggct catgctggta
    25741 ttacatccgg ttttgcgacc cgcgtaatgc ggcgcgcttc atcgacccgc aggccgagcg
    25801 ctattggctg ggcggacgaa cggccggcgg cgcaccgcaa ctgggcggag tcgacctgta
    25861 tatgggcggc gccgagcacg cggtgttgca cctgctgtac gcgcgtttct ggcacaaggt
    25921 gctgtacgat ctgggctatc tgtccacgcc ggagccgttt cacaagctgt tcaatcaggg
    25981 catgatcacc gcctacgcat accaggatgc gcgcggtgta tacatccact acaacgacat
    26041 cgattttcgc gaagacggcg cttaccacaa gcagacgggc gagaagctga ccgaaaccaa
    26101 cgccaagatg tccaagacgc tacgaaacgt cgtcaacccc gatcacatta tcgaggagta
    26161 cggcgcggac accttgcgtt tgtacgagat gtacatgggg ccgctcgagg ccagcaagcc
    26221 ctggaacacg cgcgacataa tcggcgtaca ccgctttctg cagcgcgttt ggcgcctcat
    26281 gattcagccc gtcgacgaga acgacccgga gggcgcctgg cgcattaacc ccaaactggt
    26341 ggataaggac aacgaggcgc tcgaacggct gctgcacaag accattcaaa aggtcggcag
    26401 cgatatccaa cgcttctcac tgaatacggc catcgcgcag atgatcgtct gggcaaatga
    26461 ggccggcaag gcacagacgg tcgggcgcgg ccagtgcgca cgtttcctga agctgttggc
    26521 gccctttgcg ccgcacatca gcgaggaact ttggtctcgg cttgaacagc cgcggtcaat
    26581 agtcgaggac ggctggcccg acttcgaccc ggccttggtc aaagacgacg ttattgagat
    26641 ggccgtgcag gtgaatggaa aagtgcgcgg gcgcatccag gccccggtgg acgccgatga
    26701 tgccgaactc acccgcctgg cgctgcagaa cgaggcgatc gccaaagctg tagcgggccg
    26761 gccggttcga aagaccatcg tggccaaggg gcggctgatc aatctcattc tcgcttgacg
    26821 cctacctgag gtgcccggtc agacctgacc tcgatcgagg gatccaggga tcaagggatc
    26881 aagaggtcgg ggcgtagcag agagcagagc cggggcgtgc cgcccccgat tgccgccaac
    26941 cgtacggcgt gtgggctcga tggatccgag cccacagcgc cagcaagaac aaacaaacct
    27001 tctcgctgcc tcgcttgcgc gtcgggctcg gatcgttccc ctggcttgcg ctttggattc
    27061 gactggctcc aagaatccat aaatgcgcgg gggcgggtcg cgacgccgcc aagaacacga
    27121 tcgactggag agccaaatgc gaggccgcgc cgtggcagaa ctggctctta tcggaccgta
    27181 ccttgcatcc ggttacgtga tgcgtttgga ttttagcatc ggggcacgtt attgcgtggc
    27241 ctgtctggtg ggaagcgagg gcgtgagatg caactcggac ggtctgcgga agcactggcg
    27301 gtcgtgctcg ccgtactact gaaccttggt gcgcttccga agggcgcgcc tgtaacgcag
    27361 gcatgcgctg cactggacga attgcccggg ccggggccag gtccggtgcc ggggccgcct
    27421 gcgcactcgg aatccccgat gcagccgtat cgaaagcacg cggcgcaggt gcgcatagac
    27481 cgcgtggcgc acgaaggcga gcaggcgttc gacctggagg agggcgtagt tttcggcgat
    27541 ctgagcgggg cgtagatcga ctgccagggc aacgtgcttt tcagcgccgg gctggtcggt
    27601 cccggcgtga ccgaagccaa taattgggcc atcttctacg gccaccgggc gacgtgcggg
    27661 tggtgctgcg cgaggggggt ccggccccct gcatgccgga gggggtcata atcaccgact
    27721 tgtactacgc gcgcgagcgc ttgtcggaaa ccgctcccgg ctgtgattcc gatgacgagc
    27781 cgggctggat catactgacg gcggacgtca gcggaccggg gatcacgccg cacttcaatg
    27841 accgcatttt ctacgtcggg ccgccggacg atctccagcc ggtgttgcaa agcggcgact
    27901 agacgccggg ctgcgagcct ggggtgtacc tggacatggc cctcaacttc ggggcggccg
    27961 taagcgatca cgcaacgatt ctggcctgca ccgatctggc gggtactggc gtgacgttgt
    28021 tcaataatta tgcctgctgg acgggcacgc gtgagagtct gcaacttgcg gcgcgaaggg
    28081 ggatgcaggt accggattgc ccgacgggcg tgattcttgg gtccccggcc cgggtggcgc
    28141 acaacgacgc cgggcaattc acattcggcg cggttctacg aggaactggc gttacggttc
    28201 tcaatgatgg tgggcgttgg atgtttgatc ccggcgcggg cctgatcaag atcgcgcgtg
    28261 aaggcgatcc gataccgtgg ttcggcggcg ggcaaagctg gaaggtcatc ggcggctcgc
    28321 tcggcactat taatgtttgg tctgacgggt gaagtctggc agcattcagg gtgatgggtt
    28381 tacaatcact gggcttgcca ccatgaacgt tacctcggcc atgaacgacg tcggcgacat
    28441 cgtcggtccg acgtgcatcc aagggccggg ggtgatcgag aatgtgaacc acgtggtcct
    28501 ttggattcgc caccacgtgc tgaagcggtg gattccgctg ttgcgcagcg gggaccaaat
    28561 ccacggtcgc accgtcatcg ctggttatga gatcgacttt ggcctgagcc ccagcggcgg
    28621 tgcggacggg gcatggcaga acctgaacga ccgcgggatg gtggtggatc gtttttcgtt
    28681 tgccgacgga agtgacggca tttatcgatt gtcgctggtg ttggctgacg cggatcgtga
    28741 cggcgatttg gatgcggatg attggacgcg tttgccggcc tgtctttgga cacagttggc
    28801 gcgacgcttg gtccagactg ccgcgtattt gatctgaacg acgacgatcg cgtcgatctg
    28861 gccgatgtgg ccatgcttca ggagctcttt caggattagt agcagagccg gctcgtgtcg
    28921 agccggaatt tgaccggagt ggcgccagtc gcgaagttgg cacgcgcaac acggactggg
    28981 ttgccctcgc tggcccttcg ggctcggatc cggtgggctc ggatcaggtt ccgcgccaat
    29041 gcgaaggctt ttggcggcat ttcggggcga cacgccggag tcgcggggcg ggcaaaggcg
    29101 cggatggcag attcttggcg gcccctggcg cgaaggtccg gcctaaccgc ctcttagcag
    29161 atcggctgcc ggcaacatgc aatgaatgaa tatatcgatg tcaaatgcgt ccactttccc
    29221 gtcgccattg acatcattca cacaccaaaa gtggcaggcg ctctcgccca cataggcgct
    29281 ccaggcagct tcgccgcccg agaccgccct gatgaagggg ttgatgtcga acacggtaat
    29341 cacgccgtcg cagttagaat ccccgatctt ttggctgccg cagggcggta ttatcccgtc
    29401 gcgggcgacg cagccgaggt cttcaaccag tagacccagg tccatcaggt ctatgcagcc
    29461 gttcttgtca atgtctgcat tgggattgaa tcccgcagcg ccccggcatg tgccgagcgc
    29521 acggtacaga tgccgcaggt ccggtgcgcc cacccaaccg tcgccgttca catcgcccgc
    29581 gcagctattc tgccatattg caacctcgag ccggccggta gaccggccca gaatgccgcg
    29641 gtgggaggga ccgaacgcgg cgaagacctc cggcagaccg ttgccgagcg cgtcaccggc
    29701 ggccaaattc cgcggccgca gaatctggtc ataaggccag ttttcgaacc cgctcgagtt
    29761 cagcggatcg ggtgccactt tggtccaggc gccggacgcc ggacgatacc tggcaacctg
    29821 cacctgattc gcatctgcgg catggccacg cttctgcttg gtcagcacgc ccaaatagcc
    29881 gtcgccgttt atgtcggccg taaccagggt cataccgcgc tccggcgaca ggccggcgaa
    29941 accgccgcac tcaaactgga aggtcaggtc gacctggcta ccctgggccc ccgcccaata
    30001 ctgaattttc gtgccgtcca aatcacttaa aatgacaccc gggttgcgcg acgcatccgg
    30061 ccgaacggcg accacatccg agcacagcat gtcgggatac tcgtggtgca cgtgaaactg
    30121 ccgcgtatcc ggatccaagc cgtaccagat cgtgagtatc gggccgcttt cgtagggcgg
    30181 ttccatcgtg ccggtgaagg ccaggtcgct gcggccgtcc ccgtcgaaat cggccacggc
    30241 caagccgcgc gtccactgcg tgaagaccgg atcttccagg gcctcccact caacgcggta
    30301 catgttcgcg atcgtgtcgt cggtcttcca gaagtggatg atctgaccgt cgatctgcag
    30361 gatcgggtcg gcgatgtaga cgagctcgtc ccggccgtcg ccatcaaagt cgcccgccag
    30421 agattccggg ggcgtaataa agttcccggt tgtattaaag taaaacggga acatcagata
    30481 ttgctgatac gaggcaccgc ccaagttttc gataaaccag agttcgtccc cgaagaaagc
    30541 gtgcaccgcc agatcgaggt cgccgtcccc atcgaaatca ccgatcccga tgtcggccgt
    30601 gccgttgcgg aatatcgaga gcgccggaat ctcgtagtcg ggtatatata ggtttaggtc
    30661 agctacgacg gtgaactcca gcccgacgat cggagcggcc gcgagtcggt atatcacgag
    30721 gcggcgggcg ttgcggctgg catcctgctg atcggttgcg aaccaggcca ctgccaactc
    30781 gagctgaccg tcgccatcaa gatcggcgac ttcgacgtcc gatatggttg caaattcgaa
    30841 atccgatccg atctgagcaa cttgcagctt aattggcagc ggcttgagaa gaacagactc
    30901 gagtgcggga atgtacatcg caggcgcctc ggtgcagcca ggcacatcgg ccgcggccgg
    30961 aggcacagcg cttacgaata gcagggccgc aagcgcggac agcaatccgg tcagcgcacg
    31021 accgcctccc tgtaagtggc gaagagcgcg gaaaagcaaa agccgctggt tagtgatacc
    31081 accccgccgc aaaaaaaacc aggagcagta acccgaaccc ggacggtcct ggtgtgatct
    31141 cataatgtgt tccaccgcca atcttaagcg tggtaagggt gaagtacaaa cgagaagtaa
    31201 tgccacttgc cgagctgggg aggtctacaa cgcgatcgac caccaggagc atcagcgctc
    31261 caagagcggc agagcgtgcg ttcatgttcc gtcctctcct gagacggcag cgctttccgg
    31321 caagcggccg aacgcgacgt ctccatcccc ccaacacccc cccaccgcac ggaactggtc
    31381 cgtgccaatc gcccccctgg ttgtgcacgg cccggccgcc caaggccagc ccgggcgaag
    31441 aatcatcggc ggcgagcggc ggccagagct accagaatca gcagcatggc cgtaggctcg
    31501 ggaataatct cgaaattcgc cgatccgctc gccatcgaca ttggtgagag attgaaggcg
    31561 atttgcgagc tcatgctgcc ggcgaaaccg atgtacgtat actcaccggg ggcggcgctg
    31621 ctgaattcag cgacctgcaa catcgccggc ggtactacct cgatcgcgtt gatgccttcc
    31681 gcaaaggttg taccgtcagg aacgagaccg ttgtattggg tcaggtaggc accgccgccg
    31741 ggccctgttc ccgtcagcaa cgctcccgat gtcccccaat cctgcagcgt gaatgcggcg
    31801 tcggcccgcg cccaggcgtg ctcaatatcc gggaagtcaa gcaacgccga ggtgatggtg
    31861 aaactcgtca tttcttcatc agcttggacc gcgaagccca aattcacttg tgggtcagcg
    31921 taaacggcga tgctggcagg accaaacgtg ccgagcaacc gctcgccatc gcggatttcc
    31981 ataggcacgt cgatcgacca gaagaagtta tcgccgtccc agtacccgcc ctcctgggtg
    32041 atcatcagcg tcgcagaact gcggccgcac gtagccgtga cggtcagaat cgtctccgac
    32101 acagcagcaa gcgccagcgg cgaggcggcg actatcccga ggagcgcaat gaatagcgaa
    32161 accgtgcaac gacctgattt ccccggtgtg aacatcagaa atcctccttc tcctactggg
    32221 cagagcggat caatacccat taaacccacg ttacatctta gttgggcaaa gtcaaggcta
    32281 attttcggac tattaggtcg ttgctaccgg agggagcgtg ggaaagtggg aacgtgggga
    32341 atgtgggaag gtgcgcgcgc aacgaccggt aacttcgagc gctcagactc agcggcaagc
    32401 ccggaggtgg cctcggagag ggcgggcagg atatggcacc tttttagtgc ttattggcgt
    32461 ctattatgcc attgtggagg gttcaaggcc tcttgacgat tttttaacga gattggtgtg
    32521 cttggccgat aaaattggaa gcggtacagg attgattggg cttgcaaaag aaaacttgcg
    32581 ccgatatgtt aatgtcagaa agtgtagagt ctggcagccg cgtccgccaa ggagagtcgg
    32641 cgcgcccgag gcgcgagcgt tgtgaggcgc aagctcgcgc caagtgtaag tgggcaacgg
    32701 agtgccgcgg ctgctgttag cgaactatcc tggacagaga cagagcatat gtgccggccg
    32761 aggctgacag cctggcgcac gcgaagggcg ggggcagcat tcacacttgc tgaattgctc
    32821 gtcgtaatgg ggatcatcgc tctgctgttg tcgatcaccg ctccgaccat acaggttgcc
    32881 cgccgccagg cgcagcaggc caagtgcgcc tcgaatctta aacagctcgg tttcgcactc
    32941 gaaaacctgc ggcaaaacta tgatgggttt tacccgctgt gggacgacaa cagcggtccg
    33001 acgcgctata cctggatcga catcctggtg cagacgcgcg tcctgggctc gcatcaagcc
    33061 ggctactgtc ccgccgatct taagcctgag cgtaataatg aagcgcgagc acagttcttc
    33121 gggctgatct atcccggcgg cggcatggcg ccgggcgttg actacagcta cgggataggt
    33181 gtgccgctgt cggccggcgg ctggagatgg cagcccagat tctcgcccgg ggacccgatg
    33241 cgccgccagt ttcacaatta cgatcgctac ccatcggaga tgctcctggc cgcggacgct
    33301 tgctggacca gcgtttacaa catgagcggc gactatctgg tttcgggaat ttggaacgac
    33361 ccgacgcaat tcgataacac gatatcctgg tcgcgccatc cgcgccaccg ggccaacatc
    33421 ctcacccagg attgccacgt ggacacggtc gagtacaagt tggatgcacc cgagcccgtg
    33481 aacacgctca agcatttcgt ctggtacccg ggcgaaagca ggaacgtgaa ccccgactac
    33541 gaggatccgt acgacgaggg aaacgcctac cccaacgtcc cgcccatcga ttggagcgac
    33601 acgagcaacg acgggtcatc gatctttccg aacgacctta acccgcgttt ctacacgacg
    33661 cgtcagacct ggacgcagat tcaccacaag accgtccggt gaaccggccg actctccccg
    33721 aaccgactca tacagcgggc gccactacgc gtgattgatc agcagcacgc ctgtcccggc
    33781 gatgcgatcg gctttcaagt cctgcaacgc gcggtttgca tctgccagcg cgtaacgcgt
    33841 tacgtgcggg cgtatgggga tttcagcggc ctcggctaac agctcgcgcc cgtcttcacg
    33901 cgtgttcgcc gtgacggagc gcaggtcgcg ctcataaaaa aggtgccgct cgtactgcag
    33961 cgttgggaca tccgtcaggt agattcccgc cacggccagg gtcccgcctt tcctaaggtt
    34021 ttcaagggcc ggcccgacca gcgtgccgac cggggcgaac agaatcgcac tatcaacttt
    34081 gaccggcatt tccgctgggt tttcgccgac ccaatccgcg cccatctcgc gcgccaatcg
    34141 ctggtgattc tcgccgcgcg tcgcaacgta cacttggcac ccacggtgca aggcgatttg
    34201 gattaccacg tgcgcagagg agccgaaacc gtacaaagcc aacttgccgc ccggcggcag
    34261 gttgctgcgc cgtaacgcgc gatacccgat aatgccggcg cacaagagcg gggccgcttc
    34321 ctcatcggcg aaagccgcag gcaacgggta ggcgaaatcc tccggaacga gcgcgtattc
    34381 cgcatatccg ccgtccgcgt cgtagccggt gaaattctgc tgttcacaca gattctcgcg
    34441 ccccgttcgg caatattcgc acgttccgca ggtatgtcgc aaccaggcca cacctatccg
    34501 ctggccgagc tgcagactct tgcagccagg gcccagcgca tccaccgttc cgaccacttg
    34561 atggccgggt atcaccggcg ttttgtgctc cggcagatcg ccctcgatca cgtgtaggtc
    34621 ggtccggcaa atggcgcagc aatgtactcg cagccgcacc tcgccaacgc cgggttgtgg
    34681 catagggcgg tccatcagct tcagtggagc tgtctggacc ggcgcaattc gctccagaac
    34741 catggctttc atgcctgtct ccatgtgtga gcggggccct cgtgcgtccc ttttgcattg
    34801 cttgccgcat agaatagcat agcgcgcgtg ttcgggccct gatttgagag gattttcatg
    34861 agcagagggt gcttggcaaa cgcgcatcgg ctgtggctac taccattgca gttgaccttg
    34921 atcgtggcgg gcgcggcttg ggcgcaacac acgcggcccg ctcccgcgca gcaggcgaac
    34981 aaaaccggag aaagtgccat gaagatcaca gtccggagca cggcttttga gccgggtaag
    35041 cagattccga agaagtacac gggcgagggc gaggaccttt cgccgccgtt gacctggtca
    35101 ggagcgcctg cggaaacaaa ggaatttgcg ctcatctgcg atgatcccga tgcaccgcgg
    35161 ccggaaccgt gggttcactg ggtaatctac ggaattcccg gcaagctgag cggcttgccg
    35221 gaaggcctgc cgaacgagct tcgccttccg aacctgcccg ctgtccaagg caagaacacc
    35281 tggaacaagg tgggctacaa tggggcgatg ccgccacccg gccacggagt ccatcgctac
    35341 tatttcaagc tttatgcgct ggacgcggaa ctgaagcttc agccggggtt aaccaaagac
    35401 gagctgttga agcagatcaa gccgcacgtg atcgccgaag gcgaattggt gggaacgtac
    35461 cagcgctaga acaggcggcc gcctgcgcgg gcttgcggac gcaggactag cgcacgagca
    35521 attcgtccaa ctggccaacc gtcgtggccg gtgggaaaca ggtttgatcg cgacagacgt
    35581 aaacggctgg acttccgtcc accgggcgct tctcctgcaa cgcgggcgcg gcgatgttgc
    35641 gttgcggatg cgcgggatcc gcgtgcagca ggacgcgatt cggcaggtac cggctgtgaa
    35701 tatggcgcca tagctgctgc gcgccgtcgg acgactggtc accgacgacc gtcacttcca
    35761 ccggcccagt cagcgcgaat tcggccgccg ccaggaaacg cgcgccggcg gtgggaaact
    35821 ggagcacgtc ggcggcgaag caactcatgg tctgatccgc ccaatggcga agcgaccggt
    35881 cgccggttat cgcgctcagc cgcaacaggt tcatcagttg catcgagttg cccgcgggtg
    35941 tggccccgtc gcgtgcgtct ttgaagcgtg cgatccgcgg ctcgtgattg cttggcgcga
    36001 ggaagaaacc acccgcgcgg cggtcgccga actgctcgat gaccgtgcgg ttaagctcca
    36061 gtgcctgctc gagccagcgc ttgtcgaagg tcgcctcgta cagctccagc aaggcctcga
    36121 tgaaggcggc gtagtcgcta aggaaggcga tttcgcccgt tcgcccgtcg cggaacacgc
    36181 gctgcagccg gccatcgcgg cactgatgat cgaggatgaa ctgggcgccg ccgatggccg
    36241 catcgacgca tttgcgctct gacaaggccg ccccgccgcg cgccaggctg gcgatcatca
    36301 gagcgttcca ttcgaccaga atcttgtcgt ccagcgccgg cggcacgcgc ccggatcgca
    36361 ccgccagcag cttccgccgc gccgccgcca gccgcggttc caattcagcc ggatcggcgc
    36421 cgcggttccg cgcgaattcc tccaggctgc gcgggacgtg cagcacgttc tttgcctcgc
    36481 cgggcgcatg cgggtcgcgc cagttgcctt ccgcggtaac gccgtagtaa tcgcagaaaa
    36541 gctccccctc ttctgggccc aggctggcta gaatttcggc cttggtccaa acgtagtact
    36601 taccctccac gccctcgcta tcagcgtcgc gcgcactgta gaagccgccg cccggggctt
    36661 gcagctcgcc caacacgaaa tcgaacagct cgcaggcaac gcggcggtac agcggattct
    36721 tcgtgaccag gtgcgcatcg acgaaaacgc ggctgacaag cgcctggtca tagagcatct
    36781 tttcgaagtg cggaacgtgc caggccgggt cagtgctgta gcggtggatg ccgccgccaa
    36841 gctggtcgaa gatgccgccg cgggccatgt ggtcgagcgt caggttcacc agcgctaata
    36901 gctcacgcga ttgcgcggcg tccgggccgt gccggtgtac cagccgcaag aacaagtcga
    36961 gggccagact gggcggaaac ttgatcgtgc cgttgttgag catcccgccg cgctgccggt
    37021 caaatccgcc ggccaccgct tgggcggcgc ggtcgatccc ctcctgtgtg agcgccgccg
    37081 ccccgtgacg gccgggctcg agcgattgaa tcacggcctc ggtcagccgg tccgcctcgg
    37141 ccagcaggtc gtcgcgccgc gtcttccaaa gctcgccaat tcgccggcac agatcgcgaa
    37201 aaccaggccg cccaaaggcc ggctgcggcg ggaagtacgt cccggcgaag aacggccgca
    37261 ggtcgggcgt aagccagacg ctcatcggcc agccgccctg gccgcgatta aggatcatcg
    37321 tcgcctgcat ataaatggcg tccaggtccg gccgttcttc gcgatcgacc ttgatgttga
    37381 cgaagttttc gttcatcacc tccgcggtgg cttcatcctc gaagctctcg cgggccatca
    37441 cgtgacacca gtgacaggcg gagtagccga tgcttaggaa gatgggcttg cgctcagtgc
    37501 gcgcccggtg cagcgcctca cggcgccatt cgtaccactc gaccggatta ctggcgtgtt
    37561 gcagcaaata aggggaggta gcgtgtgaca ggtgattgcg tcccgttcga gtgccgacag
    37621 ccatggagag tcgtcctgtt agaggtccgg ccagttcgcc tggcgcttgt ctcggccatc
    37681 aggatttttt gggcatgata agtagagtca agtatccgga atgcgctgcg gagaagcgcg
    37741 gcgcgcggtc ggggcgctgg ttacagtttg gtaatacctt attcacgggc acttcagtcg
    37801 cgcggcgtag tttgtgcgcg ggtgccgttc cttgcagacg gctgtcaaat tggcgggctc
    37861 gcgggcgggc gaaaatgccc ataaagcttc gtaacctttg tttttgccgg gttttatggg
    37921 cgtggccggt gttggcacgg tcgttgcatt aggccggttg gaaatgaaag tactgcacga
    37981 acaacggcac ggataaagga gaaatcgcat ggtggctccc tactcgctaa cgccttttac
    38041 ggagttgcga cgccagttgg atcaactttt cgatgagttc ggattcggac gctggcgtgc
    38101 gccgctgatg acagcggctt acccactggt gaacatttgc gacgtggggg aagaactgca
    38161 cgttgaggcg gaattgcccg gagtaaagaa ggaagacctg gaaatcttcg ctctcggcaa
    38221 cgagttaacc atcaagggcc gctggcagcc ggccgccggc gacgggcaga ccaggcatcg
    38281 ccaggagcgc ccgagcggcg agttcagccg cgtcatcacg cttccggtcg aggttgatga
    38341 ggacaaggtg gccgcagagc taaaggatgg cgtactgtcg gtccgcttgc ccaaggtcga
    38401 ggcggccaag ccgcgcaaga ttatgctgaa gtcctagaac gatggccatt tggaacgatg
    38461 gtcatgtaga aaaggagcag aaacatggtg gataccacgc tacagaagcg cgaagcccag
    38521 cggggcgagg ccgagcagat tcgcagcggt ccgatgttca tccccgccgt cgatatcatc
    38581 gagacgaaca acgaaatttt gctgctggcc gacatgcccg gcgtcaagcc cgacggcatg
    38641 gacattaatt atgaggccgg cgtactgacg atctacggac gcgtcgagcc gcgccgcaag
    38701 ggcgacgagg actatctgct cgaggaatat gaggtcggcg atttcagccg ccgcttcgaa
    38761 atcggcgaag ggatcgacgc taacaagatc gaagccgagt tgcacgacgg tgtcttgacg
    38821 ctgcacttgc ccaagacgcc ggaagtgatg ccgcgaaaaa tttcggtcaa agcctgacgc
    38881 cgtcttgcgg cagttgggta tgcgattgga ggtaacaata tgaacctgct acctagacga
    38941 agccggcgtg agttgtcccc gttgtcccgg cgcgacagtg gtctgacccg ctttcgcact
    39001 gagatggaca acctgttcaa tcgcttcctt ggcgaaccct ggggattcat ggaaggcctg
    39061 gccacaccgc ggaccgacct ggccgaaacc gacaagcacg tcaagatcag catggagttg
    39121 cccgggatag acccaaagga tgtcgaaatc agcgtggcgg gcggcatgct gacggtgcgc
    39181 ggcgagaaga agcaggagcg cgaggaagag cgccaggatt atcactacgt cgaacgtgaa
    39241 ttcggcagct tccagcgcac cttccagctc ccgacttcgg ttgaccccga caaggtggac
    39301 gcgcagtacc gcaacggcgt gctccacata acactggaaa aacagccgca ggcccaggcc
    39361 aaacgcatcc cggtgaagac ggcctgaagc gctgcgtcct ggcgttgagg aacgctaagt
    39421 cgcccctacg ggacttgccc cagctcgcct gaggctggtc ccgtagggac gactgaacat
    39481 agcccagggg gcatcgggtg ccatgcccga gcgcgcatcg ggtgccatgc ccaagcgcca
    39541 agcgcgggca tgccagcgac agtcgcccga cacctgccac gaactccggc tgctcacggc
    39601 agcgcggagc tcgcatttta ccgggcgcgc gctgcttgcg gtttggagcg caatgctcgc
    39661 aggccgaagc ggcgccagcg gcattccagg cattgaggcg ttctgggctc catcgccgat
    39721 gccgggattc cgcaggccaa gtctgcttcc gtgtcttcac ttgcgagacc ggctggttca
    39781 agcgtcggca actcgtcgtt ttccaaactc aggccgcggc tcaacgttct gagcttaccg
    39841 ggattttcaa gcagcagcgt caccgcggcc atgatgtaga aggccgagaa aatggcgtgg
    39901 atgtgctccg ggcggaggcc gaaggcggcg gccggatggg ctgcgaccac gcctggcaag
    39961 gtcagttggc ctacgaacaa cgcgaacagc agcacgccgc ccgagaccga caatcgcagt
    40021 gaggccagca acatgaccgc cagcagcgac tgcgccgcgg tgagcagaat ttcctgcatc
    40081 tgcagcccat tcatggggat cgggtgctcc aacgtgccgt gcgagagtgc gtacacgccg
    40141 ggaatcatgc ctaccagcaa cgtccattga ttaattttgg ctgagagcag ggtgcccagc
    40201 gccatggccg cctggccgcg ccaggcgaac atcagggcga cgatgaactc gggagcctcg
    40261 gaggcaattg gggccagcca ttgcaccagc acgaactcgt ttatccgcag caggcgtccc
    40321 gtgccgatca gaccttcgca aaacggcttg gcgttcgcca ggatcacggc gcccgcgacc
    40381 agaaacagca ccgcggtcgc ggctcttcgt tgccttcgcg gcagccggat cagcatttcg
    40441 gccggcccaa ccgcctcgga ctcgacgcac ggtcggcggc tggcaagcac gatgtaccag
    40501 acgtagatgc cgagaaagac gaggccgtcg taccaggcca gcgtgccctt gatgggtatg
    40561 acgaaagcgt acgcggtggc cagcccgagg aagagcaact cggtgcaacg ctcgtgaccg
    40621 atgcgcacgg ctcggcggaa gcgccaccag tagagcgctg cgaccagtcc ccaggcgacg
    40681 ccgatcagca gccggttcgc gccggtcatg ttcgcgaccg cgtattgcgc gtacgcggtg
    40741 ccctcgtgtt ggccggcctg ccacgtgaag tacatatcaa cggcgtactc cggcaggacc
    40801 gtgagcacgg ccacgacggc cagggccagg gcctgcgaca cgtcggcttg ggccgcgtca
    40861 caggcccaca acagcaggaa cgacgcgccg acaatcgcgg ctccggaagt cgcggccgcc
    40921 agggccgggt cgagcggcac gccggccaag cgtaggacgg tgcctggcag ggctgcggcg
    40981 atggcgatcc caaagggcag caagggatgg cgctggcggg ggaatccgcg gcgcttggcc
    41041 gtcagatcag ctaaggcagg caaaggcatc gatccgatga tacgaaaaat tctctgctgc
    41101 cggaatagcg ccggagcggt cgcttggagg ttgtgggcga taatttcggc gccggcgggg
    41161 gcgcgtggtt gctcaggtcg ccgggtcatt ttgatttttg gccgggtatg ccgcgtgtta
    41221 tactgtggct gtcgtcaggt gccgcccgat tcttcggtgg tttggtcgca cgcactatcg
    41281 cggaggtgga taatgaagct cggtactgcg ggttgggtct gcatttggtc agttgtatgc
    41341 gtgagcgtcc tgccgggcgc cgtggcggat acgttgctgg tgccgagcca gtacgcgacg
    41401 attcaggagg ctatcgaagc cgcgctcccg ggcgatgaag tcgtgctcgc cgatggcgtg
    41461 tacatcggtc ctggcaacac ggaccttgat ctcggcggaa aggccatcac ggttcgctca
    41521 gccagcggcg atcctgcgtt gtgcattatt tattgccggg gcgccgggcg gggcttttac
    41581 ttccatagcg gcgagacgtc ggatgcggtt gtccgggacc tgatgatcgt ggagggcgcg
    41641 ggcgagtatg gcggaggcat ctactgcgag aatgccagcc cgacgctgat cgactgcgtc
    41701 attatcagca acgcggccga gctccagggt ggcggcatgt actgccggga cgccgctccg
    41761 acgctgatcg gctgccggtt caaggggaac gtcagcctgc accaaggcgg cgggctgtac
    41821 tgcgaaaacg ccagtccgac gctggtcgac tgtacgctct cgggcaacac cgccgacttc
    41881 ggtggcgggg tcgggttatg ggaatccagc atcaccctca gccattgcac gttcacagag
    41941 aacaattaca cctacggtgg cgcgatatac tccagcgcct caatcgtaat gctggacaac
    42001 tgctcgatct gccggaacca agggaattac ggggccggtc tgtacctctc ggcttcctcc
    42061 agtgcgaccc tgacggattg tgagcttagc gctaacctgg ccaggggcag cggcggcgcc
    42121 gtgtacagca gagacagctc cgtgacgctg actgattgca cgctgaccgg caacagggcc
    42181 agctacggtg gcggtgcatt gaactgcgaa cagagttccg cggtactcgc ccagtgcacc
    42241 ctcgtcggca acacgaccga gagctctggc ggcgccatcg acttcgaccg atcacacggc
    42301 acgatgacgg cttgcacctt tgttgacaac accgcgggcc ggaccggggg cgctgtgtat
    42361 tgctacgaat caactccgat cttcgatgcg tgcacatttt cgttcaacac cgcctcttac
    42421 cagcacggtg gcgggcttta tctcagttat tctgacgcga ctttgaacaa ttgtgcggtg
    42481 acggacaaca gggcgcaata cggcgcgggt atctactctt cacggtgcaa cccagccctg
    42541 ctaaacacgc tgatcggcag gaatacggca gccgacatgg gcggcgggct cgagctccac
    42601 gagtctaatc cgacgctcga caactgtatt ctgctctcaa actcggccca ctctggcgct
    42661 ggattggctt gtttctcgtc cagccccaca ctgaccaact gcacggttgt cggcaatgcg
    42721 gcttctgaaa atggcggcgg tatttactgt ggcggcgcgt ccgctaatcc gattctaacc
    42781 aactgtatcg tctggagcaa cgctggtggc cagatatccg ctccttatgg tggcaacccg
    42841 atcgtgacct actccgacgt acaaggcggc tggcccggga cgggaaacat caacgcagac
    42901 cccaagctgg cattggaata cgacggccac ctcttggcca cctcgccatg tatagatcgc
    42961 ggcacggaca ctcccgcggc tggtctgccg gctgaggatt tcgacggcaa tccgcgtccg
    43021 ttggacggtg acggcgatgg actggcgtcg gcggatatgg gggcctatga attcgtcccg
    43081 ggagcgccca gcttggcggt cgctcctgcg ttgttgcgct tcacgatagc gcctgggcag
    43141 agcgggtcgc aaaccataca gattcgcaac agcggcccgg acaccctggc atggcaggta
    43201 cactgcgacg ccgattggct gacggcagat cccgcggcgg gcgagtccgc cggccagatc
    43261 gacactgtga cactgacggc ggacgccggc gaccgcgctc gaggcacgta tatcgccaca
    43321 ctctgggttg acgcgcccct ggcctgccgc ccgcacattc ccctactcgt ggtcctgcac
    43381 gtgagcggcg cgttgcgcgt tcccgcagat tatcctacga tcgaagcagc gattctcgca
    43441 gcgacgcccg gtgacgtggt gatgctggac gacggcgtgt acaccggcga cggaaacagg
    43501 gagctcgact ttggcggcaa ggcgatcacc gtgcgctccg ccagcggcaa ccccgcgacc
    43561 tgcattatcg attgtggcgg cgtggcgcag gggttctatt tccgcaacgc tgaaggcccg
    43621 gattcggtac tcgaaggcgt gacaattcgc aatgcgcacg acgattatga cggtggcgcc
    43681 gttcattgcg aatactccag tccgactttg ctcaattgct ggatagtcag tagcaccact
    43741 ggccgatcgg gcgcgggggt ttacgcgagg tttgctgaac cacgactggt aaactgtgtc
    43801 ctcgtcgaca attcggcggc ggcttcgggt ggcggcctgt acgccgggag cgacgcgcgt
    43861 ccggttctga ccaactgcac gatcgtgggc aattcggccg gcgagtacgg cggtggctta
    43921 gcgttctcgt attctcgagg cacgctggtt aactcaatta tctgggccaa ctcgggggat
    43981 cagatttacc ggacttcttc aaccgtatcg gtgacatatt gcgacgttca aggcggcttc
    44041 agcggcgtcg gaaacaagaa tgccgacccg tgcttcgtcg atgccgccgc gggtgattac
    44101 cggctcgccg cgggttcgcc ctgcatcgac gccggcaaga acagcgccgt ccccgccgac
    44161 gtgttggacc tcgacggcga tggcaacact acggagccga tcccgttcga cgtgctgggg
    44221 caactgcgtt tcagcgatga tccggcaacc gctgacaccg gcagcggtac gcccccgctg
    44281 gtcgatatgg gtgcccacga gtaccagcgc ccggtcagct tcgccgattt cgaggctttc
    44341 gccgagtgta tgagcgggcc gggcgttact ttcgaagcag gctgcggggc gtccgatttc
    44401 cagaacgaca ttgatgtgga cctggccgac tttgtctggc tgcagcgggc gtgcgcgcag
    44461 gatggcggcc atagagcagg actaacaact aagaactaat aaccagtggg aaggtgggaa
    44521 agtgggaagg tgggagtggt gagaagcgca ctgctcgcgc tgatgggcag gacgcctgct
    44581 ggcgggcaac gccgagccgt cacgcccgga gtcaatctcg cgcggagacg ggcaatgcag
    44641 ctagcgcgct ttgccgcagc actgcttgta cttcttgccg ctgccgcagg ggcacggctc
    44701 gttgcggccg acgcgtgggg tctcgcggcg gatggtttgc ggtttggccg cttcgccttg
    44761 cttctgcatg gccgcgtcct ggtcggccgc cgcactggcg aaaccggcgc cggtggcgtc
    44821 ggccttggtg gtctggaggt ttgagtaggc caggccgccg ccggccgccg cgacacgcgc
    44881 gccggcgcct tcgtccgggc gcatggcgat ctggaccttg aagatcaggt cggtgacgcg
    44941 ctcgcggatg ttcaccatca tctcgttgaa catgcgcgtg ccttcgcgct tgtactcgat
    45001 cttcggatcg cgctcggcgt agccacgcag gccgatgccg tgccgcagca ggtccatcga
    45061 gtacatgtgg tctttccaca gcgaatcaaa ggtggacagc agcacatgcc gttcgagctg
    45121 cgtcagctcc caacgcagca tttcgtaggc gcagtgctgc aactgctcgc gcacgcgcgc
    45181 cgcggattcc ggcgaggccg catcgcccgc ttcgatgggc tggctgtcca ccgcggagcc
    45241 aaaacgctgc tgggcccact gcaccagggc gtcgccggag tacttcgcca gggcctgatc
    45301 gatttcacgg tcgagtttgc cgttggtgag ataatcctcg ttgatcgccc gcagctcctg
    45361 aaagatctgc tgtggactct tgccggcgag gtgctcgtat gtccaattga ggccatactt
    45421 gcggttgacc cagttcgcga tgcgcgtgta aacctcgtta acgttctgcc ccccagcgtg
    45481 cagggcgaac tgcagcaccg cgtcgaccgg gtactcgatc tcgcgaatct tgtaggcggc
    45541 ccgcatcttc tcgcggaaaa tctgctcggc gtcctcgcgg ttggcggtcg ccaagtcttc
    45601 cagcggcacc tcgacgccga acttctcctg cgcccaacga accagccgcg ccttggcgaa
    45661 ttggctgtcg cagtagggct ccaggccgga caggtcggct tcttcgatgc gtttctgggc
    45721 ggcctcgata agctgttcaa gcagttcgtc gggctgcgcc ttgcgaatct ggttctgtgt
    45781 cagattcaag ccgaactgcg ccgcccagct cgccaggccg cgcacgtccc actcttccgg
    45841 atcaacgtct gtatcaacgt actcgccaaa agtgcgttga atcgtgccgc gcacttcgta
    45901 agcggccaga tcgcgaacgg tcttgctgag ttcttcaacg ccgtcgcctg ggtcgagctt
    45961 gtccgcctcg atcggcacgt tcagattctg gccgacccac tccgccgcgc agatcgccgg
    46021 gtattgcggg tcatagaaac gatcgatcgc gtcggcgacc gactcgtcga tcatccgcca
    46081 gatcatctcc gagagtccgc ggccctcgac cacctgctgc cgcatggagt agaacgactt
    46141 gcgctggaag tccatcacct cgtcgtattc gagcaggttc ttgcggatgc cgaagttgcg
    46201 ctcttcgacc ttcttttggg cccgctcgat gcccttggag atgcggcggt cgtcgatcgc
    46261 catgccctcc tcgaacccca gcatgttgag catcttgagc atccactcgc ccatgaagag
    46321 gcgcagcagc tcatcctcca tcgagagaaa gaagcgcgag ctgccggggt cgccctggcg
    46381 gccgcaacgg ccgcgaagct ggttatcgat gcggcgcgat tcgtggcgtt cggtgcccac
    46441 gacgtgcaga ccgccgatgg ccgccacccc cgggccgagg acgatgtcgg tgccgcggcc
    46501 ggccatgttg gtggcgatcg tgacgttccc gtgcatttcc tttttcttgc cgcgcaccac
    46561 ttcgtgctgc tggccggctt tggccacgat ctctgcctcg cgggcgtggt gccgcgcgtt
    46621 gagcacctcg tgctcgatgc cgtagcgccg gctcagcagt tggctcagtt cttcggaggc
    46681 ttcgacgctg gtcgtgccca ccagcaccgg gcgtccgccg acgttttgcc ccatcagctt
    46741 gcggtagccc tcttcgaggc cggcataaag agccgcttcg tcgccctcgc cattgccgaa
    46801 ggcgcggatc gcctcatctg cgattcgaat ctgctcgtca atgcgctcga ggccgggtaa
    46861 ggatgccgcc tggcccacgc gcgcctcttc cggggcctgc ccattgccgt tcgcgtcacg
    46921 caagctcacc agcgtgcggc gggccatctt caacatgtcc agaagcgagt acgcgtcggc
    46981 cgggtaaccc ttctggctgt aggcgcgaat ctcctcgacc aacgcgtcca gcttgctgcc
    47041 gcgggtcttg taaatcttgt cgttgtaatc gatgcggcgc accggctggt tggtcgggat
    47101 ggcgatgacc tcgagcttgt aaatcttcat gaactcgtcg gcttcggtca tcgcggtgcc
    47161 ggtcatcccc gcaagctgcc ggtacatctt gaacaggttc tgcagcgtga tcgtggcgag
    47221 cgtctgcgtc tcctgcttga tcgtgacctt ttccttggcc tcgaccgcct ggtgcagacc
    47281 gtccgaccat tgccggccgt gcatcatgcg cccggtgaat tcgtccacga tgatgatctg
    47341 gtcgtcgacc gcgacgtatt ccttgtcctt ctgatacgcc aggtgggcgc gcagggcgtt
    47401 gtcgatgtaa tgcggccagt tcatgttctt cgtgtcgtag aacgtgccga tcgcgagctc
    47461 ggcctgagcc gccttggcgc cgtgctcggt catgtgggcg cttttgcgat ccagctcgac
    47521 cacgaagtac tgcatatggc cgatcgcttc ggcctcctcg ctcgacagcc acagcgggtc
    47581 ggcccggaat ttcttgatgc cgtcgtaaaa tttcgggttg ttccgcgcct ccggcggagg
    47641 gttctgttcg aggtcgttca gccgccgggc cgtttcccgg ttggccgcct gttgcttggc
    47701 gatcagcatg cgggcgatgt tgtcggcgac gcgatagttg ctgacgtcgt cgtgggccgg
    47761 tccggaaatg atcagcggcg tgcgggcctc gtcgattaga atcgagtcga cctcgtccac
    47821 gaccacgtag tcctggcggc cctgcatctg atcgcgggcg ctgtgcttca tgttgtcgcg
    47881 gagataatcg aacccgaact cgctgttcgt accgtaggtg atgtcgcagt tgtaggcctc
    47941 gcggcggact tcggcctcag cgccgtaggt ttccatgtcg gacgtgatgt acccgacggt
    48001 gacgcccagc aggtcgaaga tgggccggca gaactcggcg tcgcgcttca ccaggtaatc
    48061 gttgacggtg accatgtgga ccttcaggcc ctggagcacc ttcaagtaat tggccgcgta
    48121 gcagacgatg gtcttgcctt cgcccgtccg catctcggcg acattgcaat cctccagcac
    48181 cttggcgccg atgagctggc attcgaactg gcggtgattg cgcgcgcggc gtgaggcctc
    48241 gcgcacacag gcgtacgctt cggggcggat gtcgtccagc gattcgccgg ccgccaggcg
    48301 ctgcttgaac tcggccgttt ttgcccggag ctggtcgtca cttagcgctc gaatctgctc
    48361 ttcaagcgga tcaatgccct ggcggacgag ttcgcgcaag cgcagcacga cccggtcgtt
    48421 gcgtgacggc atgaactgga acagcagcca gccgttgtaa cccgcccagc cggcgaagat
    48481 taccgacgcg gcgatggcca tccagtcgcg ttgagcccag acccagcccg ttcgatccgg
    48541 cccgagcacc gccagaatga tgaaaaatcc cagcagggcg gtcagcgcga gcgacccggc
    48601 cacgagggtt cgcccgccgc gcatgcggtg ccagaagacg gccatcgggc tgaccgcgtc
    48661 gcgcaggatg tcagtgtcgc cgaccgagag aagccagttc atacagttct cacgtgcgtg
    48721 gaaaagtggg aggaaggtgg aaaagcggga acggttcgcg cctcagacgt tcgaacgttc
    48781 ctgcccacct tcccacccct cgcgagtccc tatttgccct tcgcgcgctc aatgtactcg
    48841 cccgtgcgcg tgtcgattcg cagcacctcg ccgttttcga tgaaaggcgg gaccttgacc
    48901 ctcaggccgg tttcgagctc ggcgtccttg ttctgattcg tgaccgtcgc gccctttatt
    48961 ggcggggtgg tgtcgaccac ggccaattcg accacgttcg gcagttccac gccgacgatc
    49021 ttgtcgtcga tgatcgtgca cacgacgacc tcgttgccct tcaggtacag ctccgcatca
    49081 cccatcacgt ccttcgatac gtggatctgg tcgtaattgc cctgatccat cagaacgaag
    49141 tcgtcgccgt cgcggtacag gtactcaaac ggcttgttct cgacgaaggg cgtctcgagc
    49201 acctcgtccg tattaaagcg cgcatcgata acctgcccgg tcttgatgtt cttcaaacgc
    49261 gcttggatgt agctgcgcca attgcccttg gccacatgct gcatggaatg aaccgtataa
    49321 aggtcattgt tgtacgaaac gagcttccct ttccgcagtt cgctagcttt gatcatctag
    49381 accggctcct aaatcccaaa aaatgccgca aaacaccgtc atgcgcgagt gccaagccgg
    49441 cccttccagc ccgtcgctcc tcgcggccgg cacgttgtgc cgagcatcgc aatataacgc
    49501 cgcttcgccc ttttggcgat gcgctccgtc ctcgccggcc tttcgccggc cggctcggaa
    49561 tcggatcggg atgcaccggc ttctaggacg atttcggcag gccggccgtt aattgtccta
    49621 taaattgaag ctccgggata ttttttcggc ctgccgaccg gttcacgtgc tcgcaactcg
    49681 cgatctgaac gtgagttaag cccgcctcaa gattttatgg gcgcgaaggt ggaaattccg
    49741 cgcaacgggt tcgtaacatc cattgcttgt ggaaactttg gtcggtgcgt gaggagtcga
    49801 ggagcatatt ccgccgcttt gggagccagc gagcggaaag tagccagtga ttgacccgcc
    49861 tgccgattgg aagcccggcg gcgaggtccg aacacggtaa ggtcgtccca attttcctgg
    49921 gagccggaga gttggaaaga tcgggcatcc gagcctgcgt cagcgcaccg cttccacaag
    49981 tgtgctagct gatagggagg atcggtcaat cgccggtcct cccttttatg tgtgcatcac
    50041 taggctcccc tcgaacgaag agtggtgcac cgactgggcc ggtggatggg acttgctccg
    50101 acagggccct ccaccggcct catttttgcc ccgggtagnn nnnnnnnnnn nnnnnnnnnn
    50161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50221 nnnnnnnnnn nnnnnnnnct ctgggcgtgc tcgtccggca cgatccgccg gcccgagcgt
    50281 cctgcccaca cctgcgtgaa ctccgctccg aacagcagaa taattcccga gtaataagcc
    50341 cacaccagca gcagcacgat tgatccggcc gcgccgtagc cggaggcgac gccgctgcgc
    50401 cccaggtaca ggccgatcag gagcttgccc acgctcagca acagcgccgt aacggccgct
    50461 cccagccaca cgtcgcgcca ggaaattttc acgtcgggca ggtatttgaa tacaagcgcc
    50521 aagaacgccg ttagaacgac gaagattacg gcctcgttga gcagccgcag cagcgtggcg
    50581 ctttcgccga aggctttcag cccggccgaa agaacgaaac ccaagacaag cgaggccatc
    50641 agcagcagcc ccatgagcaa cacgacggcg aacgagagca ggcgcttgcg gacggtgttc
    50701 cagattccgg cgtccggccg cggtttcacg tcccaaatcc ggttcagcgc gtgttgaagc
    50761 tgcacaaaaa cgctggcggc cgaaaagagt aacacgccca agccgacggc ccctgagata
    50821 ttggccaggg tgggccgggc gcgggcgccg ccgatgacct gttgaatgct cgaaccggcc
    50881 tgttcgccca tggccgtgac ggcctggtcg accagggttt tttcggcggt cgggccgaca
    50941 aagctggcga tccagagaag aatgagcacg agcggcgcca gtgacagcgc gccgtagtaa
    51001 gccaaggcgg ccgcatgcgt catggcgtcg tctttgaaaa actcgctcac aactcgtttg
    51061 agcaggccca tgctgttagt cctaacacgc tcgcaacaaa gtcgccgttc acgaatgccg
    51121 ctcgcggcgc gcaatgccgc tcgcggcgcg ccgaaactga tgatatgggc gggagcagag
    51181 ccggggcgtg ccgccctgga atgatgccaa cgcgtagcaa ttcgcctttg tgcgagcaga
    51241 gccggggcgt gtcgccccgg ataccgccaa caatcggcga ctgccaaaag gtgtcgtttt
    51301 cttcagatca tgctgcctga cctccggaag c
//
LOCUS       DAOSYS010000002        45669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_671_length_45669_cov_5.879182, whole genome shotgun sequence.
ACCESSION   DAOSYS010000002 DAOSYS010000000
VERSION     DAOSYS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 45669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45669
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_671_length_45669_cov_5.879182"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3346
                     /gene="dnaE"
                     /locus_tag="PLU99_00195"
     CDS             <1..3346
                     /gene="dnaE"
                     /locus_tag="PLU99_00195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOO15501.1"
                     /translation="GDRRRREAARGLRGEVAYHLLLLAQDLDGYRNLMKLTSRGYLEG
                     FYYKPRIDRELLREFSRGLLCTSTCLGGEIPQALTREDAAAAKAVAEEYLKIFGPERF
                     FIELQNHGLPEQLAINAELDGLARRLGIATVATNDVHYLTHDDVAAHDVLCCISTRSK
                     VKDEKRFKFDADQFYLKSPAEMAAALPDWPDALENTLRIAEMCNVEFDFSQRFAPKFE
                     PPERKSPDEYLRELVYAGAKTRYGQISDELRERIDYELEVIKSKGFSGYFLIVWDFVR
                     YAREQDILAVARGSGCSTVVGYCLGISAVDPLRYGLYFERFMDPERDEMPDMDIDFAQ
                     DRRQEVIDYVRRKYGHVAQIITFGRLKPRAAIRDVCRALDVPLAVADRVAKLVPEELK
                     MTIDKALKREPELKRLVDQDDTIRQVIDHARRLEGLARHASVHAAGVVIADVPLDTLV
                     PLHKPADAEEVTTQFEGPMVEKVGLLKMDFLGLRTFSQIHRACRLIEQHHGVRIELDK
                     LDLTDQRVYEMLTSGQTRGVFQFEGGGMRDVLMKMKPNRIEDLIAANALFRPGPMEYI
                     DEYVARKHGKPWTTPHPVMTEVLQETYGIMVYQEQVSRLVNRLGNVPLRRAFRLAKAI
                     SKKKEAMINAEREPFLEGAIANGVKREVAEHIFADILKFGGYAFNKAHSTGYAVVAFQ
                     TAFLKCYYPVEFMAALLTYESGNTDKIAEYLDECRRVHQPDGSTGIPVRAPDVNESEA
                     EFTVAYPDAKKAKRLKGTTPPDKPSIGRGEIRFGLAAISGIGHKAVQAICEARRDGKF
                     RDLFDFCERVDLTAVNKSVLEALIKAGAFDSTGAMRRALMDVLEKAIEAGQDVQRDRR
                     NGQLSIFGGMAAADVPRPHIGTQEWTDTEMLAYEKATLGFYITKHPLAQYEELVKSLS
                     SVDTSGLSALAERYGRGNNNGGRNKGPTVVIGGLLSKIRPVAIRQGRSAGQKMLVLTI
                     EDFVGSVEAIVFPDMLDRVRELLKPDSVLFFVGELDTRREEPCIRLNRVIPVNEARRT
                     LTNRLVVSIRSMIADDVLPQLQQACAAHRGRCPLFFEVDTRNGERVVIQAGERAAVDS
                     SEELILELGRLVGPDNVRCVGPSGRN"
     gene            complement(3397..4863)
                     /gene="cysS"
                     /locus_tag="PLU99_00200"
     CDS             complement(3397..4863)
                     /gene="cysS"
                     /locus_tag="PLU99_00200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOO15502.1"
                     /translation="MPLRVFNTLGRKTEDFVPLNPPRVGMYVCGPTVYGHSHLGHAKS
                     YVSFDAILRWLRFSGFEVNYVQNITDVGHLTDDADEGEDKIIAEARRRGLHPMAVVEL
                     FMRSYFDDMDALNILRPDISPRASGHIPEQIECVRKLLEKGCAYEVNGSVYFDVQSFS
                     KYGRLSGRDVSELEAGARVEVNQEKKHPADFALWKRAEPGHIMRWPSPWGEGFPGWHL
                     ECSVMSQKYIGETLDIHGGGIENQFPHHECEIAQGECATGKQFCRYWLHNNMVTLNGQ
                     KMGKSLGNAISLKQLFYEGHPLLEKTFEPVVIRHFILTSHYRAPLDFSNEALRGAESG
                     SYKLRDAARELSQAAAAAPAKPRADAIRAALEQTEKSFAEAMNEDFNTAAAIATVFEF
                     ARQTGTWIREGAGREDLTAADLLMRRLTQDALGLKWPETPAAAQSRLNDVMQILVDLR
                     NEARRNKNFQLSDQIRARLTALGIELRDLPEGTKWTGL"
     gene            complement(5057..5776)
                     /locus_tag="PLU99_00205"
     CDS             complement(5057..5776)
                     /locus_tag="PLU99_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HOO15503.1"
                     /translation="MAVAESWLSRNHFLVRRLHSLTGIIPVGAFLIEHLLTNSLALRG
                     PEAFNKQVQWIQEMPFLLALEVIFIFVPLAFHAIYGVAIALESKPNQNQYPYMDNWRY
                     TLQRVTAWIAVVFILAHVLHFRFAYLFGGPVYSEAPGELKYYLLTWQGFRVVFPLGVW
                     IAIYAIGTLAVIYHFCNGIATFCITWGITVGDKSRKRVLAGASVVGVILAIWAGMSLY
                     AFAAKPLPPQAADTVAGFVMH"
     gene            6026..8116
                     /locus_tag="PLU99_00210"
     CDS             6026..8116
                     /locus_tag="PLU99_00210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15504.1"
                     /translation="MPKRNQPRSLKEPRATTPAPVHPAAAAGAFAHESSWSAALNSRR
                     LHAIAVPGLLAITAGLAIASLVGDSVTFDETSHLPAGMSYLQTGDFRLAPDHPPLAKM
                     WAALPLLLVDHRWPAPDDSSWQLAFHYDFGKQWLFKLNHPDRLITPARLMMVLLLLAT
                     CLTIYALARTLFGPPAALLALLLAAFSPTLLAHGRLVTTDMPVALAAALVLLTFARLL
                     QRMDWPRLLAAAGAVAMLTLVKFSWPVVLIGPGVMIVLALARNAPLESRLLRHAPRGA
                     ADVHLLRTRRARAGGIALVCLFILATTWAAIWTCYGWRFEIIPSAPSAASGVIAEDNA
                     AVAQVYRADWHTMLNEPGGALKRARLTLSEWVRFHRLLPEAYLYGFNNAMRSAEARDT
                     YFMGQHSHVGRPAYFPLAFAIKTPIANILLVAAGILAVICKPDLRRRDPILLCGLAAF
                     GAAYTAAALTSKINIGHRHLLPLYPLLFVLGGAAVAWLSRPRLRWAIPGACVWLCGAT
                     LWIHPHYLAYFNELIGGPANGHKYLLDSNIDWGQDLKRLAQYAQQHADQPLKLAYFGT
                     ADPAAYGIQCTALPSLRQYDAAPAELTGGVYACSVNQLFGLSQPKVRDEFWADPQVQR
                     RYRELVAAAAAPPPGESSDALQEWQGMREDYDRARRWRLLNQLRHLRPDDRVGYSLFI
                     YNLTQSQVDELTRL"
     gene            8465..9790
                     /locus_tag="PLU99_00215"
     CDS             8465..9790
                     /locus_tag="PLU99_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015115059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO15505.1"
                     /translation="MWDVTTDILIILALIVLNGVFAMSEIAIVSARKTRLQSRASKGD
                     AKAGVALELAGAPDYFLATVQVGVTLVGILAGAFGGAQLGDRLADSLAGVPWLAQHRQ
                     AVALGIVVAAITYVSLVIGELVPKNIALAHPEAIARAVARPMKWLSRIGYPAIALLRG
                     SSNAVMRLLRMRAVAPVPVSEEEFKILLDQGARAGVFERVEKTLVERVFRLGDRRVGS
                     LMTPRTEIVWVDANGPIGESMRTMSAGGHTYYPLCQGNIDNVMGVVSVKSQWARMVHR
                     MPPDLREGLLTVPFVPEAMPALKLLETYRQAGRHVAIVVDEYGGVAGLVTLADVMEAL
                     VGDIPSGEARDDTAVVTREDGSHLMDGMLAVEEMQDILHLTELPGEAGEYQTVAGFCM
                     YQLGRIPRMADHFEWGGYRFEVVDMDGHRVDRVLVSPAGTSAPQAAKES"
     gene            complement(9870..11387)
                     /locus_tag="PLU99_00220"
     CDS             complement(9870..11387)
                     /locus_tag="PLU99_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15506.1"
                     /translation="MRLLTNCAICGVIVAGWALTLPAAGQTSESKLWIPDESGRFEHG
                     WFWDPTAESWKYGWFQPGNIQPRTVTREFALAGGETRTVGAVSGPVATVTAPVVAVTA
                     PMPTELRGTILNIEQRRISDFAPPQVFVTVHTEDGAVRTLDLGAATFVFRTVPDLHTN
                     QQIVLRGNIQGAAGGNIFVPIQFVTARSMLALPVAATNRQVRGALVNLYTIPGQADFE
                     KMLVAQVRTHSGDLVDVVLGSTDQVGNRFFSLRPGTPFIAEGFVEQTPSGSRLYAQNV
                     RIQEQQPAEVVVEQPSALVFSTEPVRTAPFGPAPVSPVETVQGQIVGLQTQFIGGQLH
                     LVADLTTDAGYVMRADLGPTAGLDYLGLGTGELVSVTGQRLFVDGQPILVADQLAVRG
                     QVIYVGSMPQLVGAVETAAAAPLQMYHGRIVAKRTEDIPGYRDSRLVVDLRLDDGRVV
                     PVDLGLKRCLRKMDLDRNDCITVWATSGLVDGQPGLIATQLADDDNRYIVASHCE"
     gene            11682..12191
                     /locus_tag="PLU99_00225"
     CDS             11682..12191
                     /locus_tag="PLU99_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HOO15507.1"
                     /translation="MCKLCVGTSFLLMTCLVINPAYAADKENEADKAFMTKVAQGNLF
                     EIRAGELAHKKTTSEDVRKLATRLIVDHTANNVELKKLATKHNVELPNELNEQQRKEL
                     ARLEKLSGPEFEQAYVELMIKDHKADIAAFEKQIAASQGDVKQFAEKTLPVLREHLKM
                     AEETQKKLK"
     gene            12474..13643
                     /locus_tag="PLU99_00230"
     CDS             12474..13643
                     /locus_tag="PLU99_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15508.1"
                     /translation="MPVDFGLTLAPWSFTRTAGNLLDRIVGEVGIDHLTVPAVTGPMV
                     QYRLGKEFQEPYFYTEGGWHFSPRTKLYAAVSARPHVARWAGSRDTLGPMREYAAKHA
                     LKFVVRLNFRGFRGPLMALPETRMRTAWGGELAGYGACTSSPVFRELVQATLADLTAY
                     EPDGFELDRIRSGEEELPLGVSRPLLWDGLLKYWVRTCFCPACRQIGSRAGVDVDAAA
                     EAVQSFGQDYARTGFTAALGKKLEHLGPYWDARHTDLHDWIEQIARNFAPRTVYVVSE
                     PVGPGLVTRLERDERFDARDASGPDQSPEPPAPVSFNGGSFAPYASSCEPAPITGLIW
                     NLFAPRSQAGDALVRSLSHALQLGFEFFEFERVEEMPEQAVTWLKQAVRYARRQS"
     gene            13699..14319
                     /locus_tag="PLU99_00235"
     CDS             13699..14319
                     /locus_tag="PLU99_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15509.1"
                     /translation="MPSRRKRLPRIFLYSSLCLCVSLVNYSGCGRKDRDKTGAGGPDA
                     QTAYVPPPPVKPQYTFAEGLREKHPAPAMFVQQFLETCLAGDYAGYRLLASRRREPES
                     RERFTAIYHGIRGLHVESIEQHSFPELAEEVYLVVCKVDFPPDSKVKLRRKTDKVAIL
                     AFKEEGEWRMLPAPAAMQPGSETMSESDYAPATTSAPTYPWDEEGP"
     gene            14316..15104
                     /locus_tag="PLU99_00240"
     CDS             14316..15104
                     /locus_tag="PLU99_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15510.1"
                     /translation="MSLRARYLLTAASLLFPVQRGFADTRTELPPDLDQELATIQDFT
                     YDFDHPAYYHLLAFVQNSPQAPGFATPPIVVNDWRVLVERPADYRGLPVTVEGVVGRN
                     KDPYVHPRHPELRPVSQVELSRPDQAVSCTIIFTQDVADIPIGATLQATGYFVKINRF
                     PRQSGEPGLSALIVASGPSQVSRTAGRISQTGPDWRWMVLAVLAGLIITVFMLRRARG
                     FRRHNLRELHADREPGINLAADLADWAKREPPDCPPPQKPGNAP"
     gene            15101..16240
                     /locus_tag="PLU99_00245"
     CDS             15101..16240
                     /locus_tag="PLU99_00245"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO15511.1"
                     /translation="MIVDVHCHYTLTRRAAQPADGATPLERFSFEPEIEADGTPALDS
                     FLAPRIFRRPIWSVTPLLAGLGWKPRPGPDFDRKLQAWYERHLLADGPIQRAVLLAFD
                     RYHDDAGRAPPPPERRGQLGSDMYTSNSLIRDLCRRHPNRFVFGASIHPYRKNALACL
                     DEVFAAGACLMKWLPLHQNINCEDPRTMAMLRHCAKLGLPLLMHYGEEFTLASQHPQF
                     VSVQPLLKVLRELRRTAEMPTCIIAHVATPALPWGDRGPHAALLAALLDEFADAPLYA
                     DISALTAFSKLGWLRRMARAQELHPKLVFGSDFPVPVATTLLRPWLGRDYSKIAATAS
                     WPARAIEIYRHLGFNEIVFHRAAMLLPNLSRFATDPAGAPGPAAA"
     gene            16277..17428
                     /locus_tag="PLU99_00250"
     CDS             16277..17428
                     /locus_tag="PLU99_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15512.1"
                     /translation="MRIKPTCAAILIGAAFIGGCRNLRYENTAAYLDADFQPSTLSPL
                     HIYTRNMYFVPARDILLLDPVRRAVFGSPAWNADGHEVPDGSFYTNRLPEELTPERVA
                     RGPCTEPPPEPPFTVIKKKSVGATAGFIGRDARGREYLFKPDDRCYPELGSGANVIAS
                     RIYWALGYHVPAEYVVTVSGTGDPAFEGRRASASAMIEGGKGHIAFDALRYRRELRGL
                     GLAAAWVNDTDRNDHNTLVVFEHGHARVYLVDFNSALGSWQGRPKESWRGWQPAGDVP
                     ALIGKVMTLGLWVREPDPRQPVISPAVGRFEGSHFKPAFWTPQWPNNAYAHMTSADRR
                     WIASKIALLERPHLEAIVAEARYSDPADAQYIVDTLMQRREKILRFAAR"
     gene            complement(17508..18473)
                     /locus_tag="PLU99_00255"
     CDS             complement(17508..18473)
                     /locus_tag="PLU99_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15513.1"
                     /translation="MSMRSVLRGLNVCALLVSGAALVRAETVDEVSRMLTEKMRAVTS
                     LSAKTQTVAEMSSPGYSEQRVSKGVLELLRKDGKVFLRNESREVVTSEAAGQTVKQEG
                     MALAIMDGTGYVYSYMESGDQKTAYKKKSQVDWDADPIEGLKALYDIELLPEETIDGA
                     AVCVFKLTPKAGSGGEGSALEYFRKDCGYPVKIVHFDAAGKAVMTATYTDIKLNQEIS
                     PERFVFNTPPGVELVDLTEVVVVPPEGPAAATASAPAASEAGEGSVPATQAAAAPEQA
                     APAEAAGKSPEPRRADEKAKPEKKGKAKKGDAKKDGKKGKQKAKP"
     gene            complement(18572..22258)
                     /locus_tag="PLU99_00260"
     CDS             complement(18572..22258)
                     /locus_tag="PLU99_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOO15514.1"
                     /translation="MGYEHRRCASVLGALLLLGQCCTSALLATEMDLGPAETQPVVQT
                     AVSSSTTASQPATESAPASSQPSTTQASANHEESVATITPEEIQARMASIEASTELPE
                     PMRAQILEIYRQVLEQIALAQTWSSKISEYQQGQSNAPADLEAIQQELDAVTNAPAVK
                     LQLAESTTLEDLGQKIASADSELRQKQAAADKLAAQAQNRAERAQAIPALRTAAQQQL
                     ETLSAELAGAAEAGVPPQLALAQRFLLLARRSAIEQEIRAYQEELAFYQARSEVLATR
                     RSKARLEVAQAQALLDELQAKATELRAAEVQKQKQRAAEGLKDAPWEIQRQLEINPRL
                     AAQRENVSAGLAEAQAKLEKARVDEERISARLGRLRQRVEQDSDRDLLGPLMRGARLD
                     LYRLRDAERESLETRKKYAQVQSRLSEVRERLLVLTDVDAAKRVMLAKLREDDPNIDL
                     APLEPKIREMLETRRELLEGLRTDYESYSTELLQLVDVQTRIVNSVRATREVVDRYSL
                     WLPSTAPIFKAEWPDDREGATRRLGALRRLLLPEQPEQRLRGLIVSGLALVVLLAWLS
                     VRPLLAAKVRDTGLRVAKIHTDSFRHTLVALLCSILRAACWPSALWFAAWTVRAAAEA
                     RDVAAYEMAQTLGAGLISAGMVIFFTSLVREICRPGGLGEYHFRWNAAGLRVARRSAL
                     SLMAIAAPALFVIAAMEVYPDAEVRDLIGRSAFLLLMAAVIRFLWRIGHPERGIFVDW
                     LRHHSPWLYRSRYVLLVALLMLPLLSAVGAAIGYYYTAQEMAHRLGQTARLALIVLLI
                     HALLRRAFFVAQRRLAHERARRKRAAAAARLAEQGAAHEEAPAVEDEELDLISIGAQT
                     RKLLRAVVIFIMAIGLWLCWSELLPALSVVGETKLWSYTGTEAAGQSAEGVAAVGGAA
                     ARVIKYVTLGDVTTAALVALATLVLARNIPGLLEIVILQRLPLDTGGRFAVTAVARYL
                     IIVAGVVIAFGEIGVGWSRVQWLVAAMTVGLGFGLQEIFANFVSGLMLLFERPIRIGD
                     TVTIGDISGVVSRIRIRATTITDWDRKELVIPNKEFITGKVINWTLSDSTLRIIIPVG
                     IAYQSDTELAEQLLYKVARAHENVLSDPPPRVYFMGFGNSALQFELRVFVASVDLSMS
                     TRHDLNKAIDHEFRQAGIEIALPQRDIHVRTIQQPLALLHPEHHDYGKLRGGLSLGEA
                     APEQQRDLDGILRTATPKSRDESR"
     gene            22422..23894
                     /locus_tag="PLU99_00265"
     CDS             22422..23894
                     /locus_tag="PLU99_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOO15515.1"
                     /translation="MKVLLVSPATPDTFWSYKHVLPFVAKKAAFPPLGLLTVAALLPP
                     EWELKVVDLDVRALADDDIEWADYVLLSAMMIHEKSVREVAARCAGRGKAVIAGGPLF
                     TTSPERFSEIPHLVVGEAEELMDQICRDMLAGTLRPLYRCHERPSLANTPLPRWDLID
                     FRDYATMSVQHSRGCPFNCEFCDIVEIYGRVPRLKSTQQMIGELDALVNAGWRGSVFI
                     VDDNFIGHKRRAKDLLRAIIDWRARRGVNLSFTTEASLNLVDDPELIDLLVQAGVKKV
                     FIGLETPQESSLAECAKTQNMGRDLVADVKTLQAAGIEVLGGFILGFDTDSGTIFERQ
                     LRFIQESGVVTAMVGLLTALPQTRLFARLKQEGRILSESTGNNVEAVLNFVPKLDRQV
                     LVEGYRNLVKHLYSPQMYYARALTFLRQYRPRGPRAPVRWSDVRALLHSIWTMGVWTN
                     GRREYWKFMLRSLLFHPRQFVEAATLAILGHHFRIVAARL"
     gene            24072..24395
                     /locus_tag="PLU99_00270"
     CDS             24072..24395
                     /locus_tag="PLU99_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016516510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HOO15516.1"
                     /translation="MSTTLERVWQLVATGQVKVSDHGYDELSNDGLTVREVVDGIKAG
                     QVVEDYPAYGKGPCVLVLQRDERGAPLHALWGIPRGQDGPAVLITAYRPDPAKWTSDF
                     RVRLP"
     gene            24392..24610
                     /locus_tag="PLU99_00275"
     CDS             24392..24610
                     /locus_tag="PLU99_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020861954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15517.1"
                     /translation="MNRRTTKLIREGDYVIELDVELSDSPDGWGPYLSLTDAERLDEA
                     RAALRQGDLAAATRLGRVYRLTPVKASA"
     gene            complement(24660..24847)
                     /locus_tag="PLU99_00280"
                     /pseudo
     CDS             complement(24660..24847)
                     /locus_tag="PLU99_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25236..25367
                     /locus_tag="PLU99_00285"
     CDS             25236..25367
                     /locus_tag="PLU99_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15518.1"
                     /translation="MFFNPAVGNISDCVEATVWSRSVDLIDGSEFDRRDRHRRTWAW"
     gene            complement(25703..27304)
                     /locus_tag="PLU99_00290"
     CDS             complement(25703..27304)
                     /locus_tag="PLU99_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15519.1"
                     /translation="MTPSRGWGKDTWQARAVAVLVAATLVTAIAVRVYDLAGKDLWID
                     EANSVVLAKTGVAGLLDGLKRDSSPPLYYLLLAGWMKVFGDGEAAVRTLSVIPGVLTV
                     GALFAIGRRLFGTQTGLLAAVLAAVAPTHVFYCQQARMYSLLGLLGLLSSYWLWRAIE
                     DGRGRFYVAYVLATLGALYTHNFALHLLPVHALIILASGALRRAPWHWAACGAAILAG
                     YLPWLPTFLMQLGNREQYVWFATLTQRFGSSRIPLSTLRSFSPGDPQPFFLFAREQHT
                     FWTPVYLCALAAAAGLAQAWQRGPRGLKVNPRGLWLACATVLPLACAALTSVISVPNW
                     VPGRVDQLVYPCFALLVARGLAGLHWRKAAVASALLRYCLLVVFCVYSCAGLAHLYGN
                     QLASADRAMAQIIVRAARAGDAVLCTGLTRAPLQYYLGRLAVPVKLFSYPREMALHMA
                     SQDQAAWAEQPDRLEEEIHAVEQEIAAACGPQSQLFAVIAATQPNQPLAAYLMEQYGG
                     RVEDLGVFRLSGTNTNVYLWLRRAE"
     gene            complement(27939..28823)
                     /locus_tag="PLU99_00295"
     CDS             complement(27939..28823)
                     /locus_tag="PLU99_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOO15520.1"
                     /translation="MTKMATPPGAAPAPGGKPANLYVPYRMRIAKVTDEAPGVKTFRL
                     EFVDPADAEKFTFKAGQFGEYSVFGEGESTFCIASPPTRKGYIECTFRQAGRVTSALA
                     DRDEGDVIGFRGPYGNVFPIEQWHGKKLLFIAGGIALPPMRCVIWNCLDLRDKYGDVS
                     IIYGARTVADLVYKHELAEWGTRKDVNLSVTVDPGGETPDWKGKIGFVPTILEQLAPS
                     ADNTIAIVCGPPIMIKLTMPVLAKLNFKPEQIYTTLENRMKCGVGKCGRCNVGKVYVC
                     KDGPVFTMAQLSQLPPEY"
     gene            complement(28820..29839)
                     /locus_tag="PLU99_00300"
     CDS             complement(28820..29839)
                     /locus_tag="PLU99_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO15521.1"
                     /translation="MSETRILQKKSLGELFAKLTAAGQRLLAPVKEGELLNFAEVKSP
                     EQVALDYIQTRSSAKLATFPPYEPLLRYKFNGKDVEQQPAEPDKRPTVLFGAHPCDAA
                     SFATLKAVFTWDSPDAQFETRLANLTIIGLACTKADAYCFCTSVGGSPGDTRGSDMLL
                     TPLDAERFLVEVLTPKGQAVVAAAPQLFTPAGNETKDKVLAKVAPRFSADKLKEKLPA
                     AFGNNAVWVEQSLRCLGCGACAFVCPTCSCFDIQDERNREGGTRLRCWDSCGFSLFTL
                     HASGHNPRHVQSERWRQRVMHKFSYQPERLGVLGCVGCGRCSRTCPVDMNLAEHAQAL
                     AEMKA"
     gene            complement(29844..30650)
                     /locus_tag="PLU99_00305"
     CDS             complement(29844..30650)
                     /locus_tag="PLU99_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO15522.1"
                     /translation="MEALQKKAKALLENGTVKVVIGFAAGTDAQTARPLFARTPEQAG
                     KLIWGESCKQNLATYLLKPEVKALGKAAVVGRVTTARTVMQYAAENQIADDAAVLLIV
                     SDDGQITELAKFADLEVYLAKVPRGLSPEAQKEIERLQAMPLEQRWAFWSAEMSRCIK
                     CYACRAACPMCYCARCITEVNQPQWIPVATDPLGNLEWNIVRAMHLAGRCVDCGSCSD
                     ACPQGIRLDLLNRVLAQEVLTSFGGEAGYSTRKEYALSTFKPDDKEEFIR"
     gene            complement(30650..31099)
                     /locus_tag="PLU99_00310"
     CDS             complement(30650..31099)
                     /locus_tag="PLU99_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOO15523.1"
                     /translation="MADYEPKVIAFVCNWCTYAGADLTGTSRIKYAPNIRMVRLPCTG
                     RIDFMLLLQAFGQGADGVIVSGCHPGDCHYTAGNFHARRRWIVFRRLLDFMGIDLRRV
                     YFSWVSAAEGAKWAELVNNVVADVRKLGPYDTFPKRLSGQIRPLEVA"
     gene            complement(31144..33135)
                     /locus_tag="PLU99_00315"
     CDS             complement(31144..33135)
                     /locus_tag="PLU99_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOO15524.1"
                     /translation="MPRIGVFVCHCGENIAATVDAAAVAQAAAGFPGVVHSVDYKYMC
                     SDPGQNLIRDAIREKKLTGVVVAACSPRMHEPTFRRTCADAGLNAFLCEMANVREHCS
                     WVHEKLPATTEKAIDLVRIMVEKVKRNRPLFPIKVPVTKTALVIGGGIAGIQASLDIA
                     NAGHKVILVERDPSIGGHMAQLSETFPTLDCSQCILTPRMVEVAQHPNITLYTYSELE
                     KLEGFIGNFKATIRRKARFVDEKLCTGCGICAQKCATKKIPSEFNAGLGNRTAIYVPF
                     PQAVPNKPVIDKAHCTYFRTGKCKVCERFCEKKAIRFEQEDELIEVPVGAVVVATGYD
                     LKQTDFFPEYGYGKYKDVVNGLQFERLASASGPTLGAIKRPSDGKTPETIVFIACAGS
                     RDPAKGINYCSKICCMYTAKHAMLYKHKVHSGKAYVFYMDIRAGGKMYEEFVRRAIEE
                     DEVHYVRGRVSKIYERDGKLIVKGVDTLLNSRPVEIQADMVVLATAMVAHKDAEELAQ
                     KLKVSYDGDHFLSEAHPKLRPVEMNTAGVFVAGACQAPKDIPETVAQASGAASKVTGL
                     FSKDELQREPVVAKVNRCAPPLFSTCIGCFLCESACAYQAIEREEIRGRDGKLIKTVA
                     RINEGVCQGCGTCVALCRSKSIDLQGFTNEQIFAELLAL"
     gene            complement(33139..34074)
                     /locus_tag="PLU99_00320"
     CDS             complement(33139..34074)
                     /locus_tag="PLU99_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOO15525.1"
                     /translation="MKIGYFPGCSLLGSSREYGESLTAIAPLLGLELTEVPDWNCCGA
                     SAAHSLNHKLALALPARILALAERAGLSELLVPCSACFTRLAGTRYELSKDENLRREI
                     SRIIELPYNGTTRVLNVVEVLSRAAANGLKEKVSKPFGRKVACYYGCYLTRPAALNTC
                     KRAEDPQEMDDIVKVCGAEPIDWPFKVECCGAGLSVAKLDSVARLSGQIVDDAVRRGA
                     EAIVVACPMCHTNLDLRRDAIQQYRNQQYSIPVIYISQLIGLTMGLSPDKLGMQRHHV
                     PVRFERAAQTISTPAPAAAASAAAPAQAGTAEKRG"
     gene            complement(34071..34685)
                     /locus_tag="PLU99_00325"
     CDS             complement(34071..34685)
                     /locus_tag="PLU99_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOO15526.1"
                     /translation="MSGSSHHEHDHSLAGQVKAATGESVFNCYQCGKCSAGCPLGPEM
                     DLAPNQVLRVLQLGLPALEEEALKAESIWLCLACETCATRCPQEVSIPKIMDYLREEA
                     LRRGVANPKAKDILAFHRSFLDSIKKNGRLHEVGLISAYKMRTMHLFQDVNVAPKLLM
                     RGKLKILPHKIKGRDAIAAMFDRCAAASGHAGQTTGHHDEADKR"
     assembly_gap    34813..34912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35068..36000
                     /gene="argF"
                     /locus_tag="PLU99_00330"
     CDS             35068..36000
                     /gene="argF"
                     /locus_tag="PLU99_00330"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOO15527.1"
                     /translation="MAINLRGRSLLSMKDLTGEEINAILARAAELKADRAATFRAAPL
                     AGKTLAMIFQKPSLRTRVSFETGMTLLGGHAIYLAPSDIKLGERETTEDIALVLSRFA
                     DIIMARVFGHQIVVDLAKHATVPVINGLSDFEHPCQILADLLTIQERKGRLRGLSATY
                     IGDGNNVAHSLMYGAARVGMNMTVLTPPGFEPLPQVLEESQAIGRETGATIRVTNDLA
                     DGAKGADVLYTDVWASMGQEAEAEARRKKFAGFQINSKLMSYAKPDAIILHCLPAHYG
                     EEIDYATSRTPNSAIFDQAENRMHAQNALMVMLA"
     gene            complement(36075..36887)
                     /locus_tag="PLU99_00335"
     CDS             complement(36075..36887)
                     /locus_tag="PLU99_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOO15528.1"
                     /translation="MLNLVWIATMFLIAFAQPLPSGPGVAPETQTAAAAPSSVTVTGF
                     IRKTVSVDDQSYAYVVYVPPDYTPERPWPVILFLHGAGERGDDGFLQTDIGIGRAIRR
                     HHRNIPAIVVMPQCRPNESWVGPMAGMALRCVELTSREYNLDPKRVYLTGLSLGGQGA
                     WMLAASYPDRWAAVVPICGFAEFGESTGLAEKIAPRLRDIPIWCFHGAADKSVPVEKS
                     RELVEAIKAAGGKGIWYTEYPNGNHNVWDKAYDDPDLWRWLFAQERVDRKSE"
     gene            complement(37194..37826)
                     /gene="infC"
                     /locus_tag="PLU99_00340"
     CDS             complement(37194..37826)
                     /gene="infC"
                     /locus_tag="PLU99_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOO15529.1"
                     /translation="MRRNEQIRISPVRVIGEAGEQLGVMPVNEAMRMAHEAGMDLVEV
                     APNVRPPVCRIMDYGKWKYLQKKKTKKSHEQALKEVRLRPKTDDHDRGIKLRQAEKFL
                     KAGDKVQFTMRFRGRERAHREIAFASLREIADQLGEKVKVERPPSMDGRNMIMILSPV
                     KGAWGEGGADEDEQSSPERPAVVRPPEARRPQEHVPAPQTMPATTAAEEA"
     gene            complement(37844..40036)
                     /gene="thrS"
                     /locus_tag="PLU99_00345"
     CDS             complement(37844..40036)
                     /gene="thrS"
                     /locus_tag="PLU99_00345"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOO15530.1"
                     /translation="MVKVQLPDGTVVEHPDGVRVVDVLRALNPRLADQAVAAKVDGQV
                     VELSTRLTDGQVKLTALKIDDPEGLHVARHSAAHVMAEAICQLWPNTKLVYGPPVDNG
                     FYYDIDLDYSLTPEDFERIEARMAEIVKEDRPFVRYEMPRDEAMRKLEAERNEYKIDN
                     ALRAEGDTLSFYSTGEPGKSFEDLCRGPHVPSTGRIGAFKVMQVAGAYHHGDATKQML
                     QRVYGTTWPNKKALQEYLRMMEEAKKRDHRKLGQELGLFTISPLVGTGLILWKPHGAT
                     IRHVLEEELRKELIARGYQLVYTPHIGRLDLYRTSGHFPYYRESQYPPLFESERARML
                     NRLWELAGEAGEQASPAEIELFNDLAAEHEDLRQAGYPKSAPREERQRRIRAWLAVED
                     GYLLKPMNCPHHIQIYTSEARSYRQLPVKLAEFGQVYRYEKSGEVNGMTRVRGFCQDD
                     AHVFCTPQQLPDEIAECVDLSQFVLKMIGLNDYRVRIGLRDPDSKKYIGSEENWRMAQ
                     EALRRSVAAAALNSSEEVGEAAFYGPKIDFVVRDCIGREWQLGTVQVDYNLPERFGLT
                     YIGEDNSEHRPVMVHRTLFGSMERFVGILIEHFAGKFPLWLAPVQVTVCSVSEKSAAY
                     AAQVAAACRTAGLRTELDNSSERIGAKIRSATMMKVPYILVVGEQEMRNGAVNVRTRE
                     GQQYGSFRLSDFLAACATEIATHGEKTPAPGGQAPAVEGASLTHAEGG"
     gene            40422..41906
                     /gene="gatA"
                     /locus_tag="PLU99_00350"
     CDS             40422..41906
                     /gene="gatA"
                     /locus_tag="PLU99_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOO15531.1"
                     /translation="MNSALQIATEVSAGRLKAEQVCQEALALIAQRDPSLHAFLELTP
                     EHARQRAAAIDAAVSAGQPVGPLAGVPIAVKDNICTLAGRTRCASKILEEYRSPYNAT
                     VIDRLEAAGAVIVGKTNMDEFAMGSSTENSGMGPTGNPWNADFVPGGSSGGSAVAVAA
                     RMVPLALGSDTGGSIRQPASLCGVVGLKPTYGRVSRYGLVAYGSSLDQIGPLATNVTD
                     AALLLEVAAGQDPHDSTSVSEPVPQYTAALEDSQLAQCAAGLRIGVPREYFGEGLDPE
                     VRTAIEAALEVYRGLGAKIVEVSLPHSPYSIAGYYVVATAECSSNLARFDGVHYGRRT
                     AAPKDLIDLYSSSRDEGFGPEVKRRIMLGTFVLSSGYYEAYYSKALKVRRLIKQDFDR
                     AFEQADVLAGPTSPTPAFRLGEKVSDPLAMYLADVYTISVNLGGLPALSIPCGFSAAA
                     GLPIGLQLIGPLFGEARLLQAARLYERETDWHTREPPLVAQCPN"
     gene            41894..43939
                     /locus_tag="PLU99_00355"
     CDS             41894..43939
                     /locus_tag="PLU99_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15532.1"
                     /translation="MSELEPEPRLTHEPEPAQEGPEARVPCPSSKRGHAPATPPDACD
                     QAHEDYEACQELPTPISEGASPQSDAGAPGFEDAAICGIIILLAFVLRFIYILQWQRN
                     PGFAHPGMDELYHDQWAQAIAAGKAFVEGPYFRAPLYPAFLGFIYKLFGHNYLAPRIV
                     QAVLDSLSCGLLFIAGRQVFSRRVAAVAGFAAATYWMLIFFTGELLIAPLLVFLDLLL
                     IILLLRAGKKPGALLYGLAGLVMGLSAIARPNILLFAPTVVLWMLVRHWRELRRGLVY
                     VACITVGCLLPILPVTIRNYVVGNDRVLIASQGGVNFYIGNNPWSDGRTAIVPGTPGG
                     WWEGYYASIALAEEAEGRKLKPSEVSSYYYRKGWEFITQQPAKAISLSALKLSLFWSS
                     WEIPNDKGVYFWTERFTPIVKLLPLGFGVVGPLGILGLFLSRRRGRELFPLWGFILVY
                     SASIVVFFCNTRYRMPVIPPLILLGTWAIFETVRAVKQQRWRTVGASAAVLAVAGIFV
                     NVTPGSENFRTDADDWFFLAMEYESQGHGTPALRCYEQSLQVRPDFLMARLRYGKLLM
                     RAQRPTEAIAQFRQALAAPPRLATNESQATLAEAHVLLGAALAETGAFAEAAAEFQAA
                     IPSLPPDKSIRAQVSLGRALAALGRNAEAIDVFKQVLQVDPGNDRATTELHKLGLTP"
     gene            44101..44856
                     /locus_tag="PLU99_00360"
     CDS             44101..44856
                     /locus_tag="PLU99_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO15533.1"
                     /translation="MNAEHLSQTWRTTLAFAALLVVAHPATAVTIEDWSSGTDAAWTR
                     VDLLELYGLGGTSYTVSGGQYHMTSNYPLPPVPYLLGSASVFSESLLDPDAYGNGYLT
                     TRFTIHNANSCVFVPMRADVAQMDFISFFAENASDSIGMTVFDNFEYVNGASASFTIE
                     EEVEYYLTAGAVGTELSIKVWPVGSPEPASPQVTLIDTTYTSGQIGLGAYNREGMGGT
                     MAASFGEVSFIPEPAGLLTLLAALGLLAARRRK"
     gene            45368..>45669
                     /locus_tag="PLU99_00365"
     CDS             45368..>45669
                     /locus_tag="PLU99_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO15534.1"
                     /translation="MADQQQLFPAAMKELAERRKRLAPQALGAFKAFSQAVFADGALP
                     GKTKQLIALAVAHVTQCPYCIRAHTKQSLQHGATAEEIMEAIWVAAEMRAGAAYAHS"
BASE COUNT         8195 a        15166 c        13921 g         8287 t
ORIGIN      
        1 cggcgatcga cgcaggcgcg aagcggcccg cggcctgcgc ggcgaggtcg cttatcacct
       61 gctgctgctg gcccaggatc tcgacggcta ccgcaacctc atgaagctca ccagccgcgg
      121 ctacctggag ggcttttact acaagccgcg cattgaccgg gaattgctgc gcgagttttc
      181 gcgcggcctg ctctgcacca gcacctgcct gggcggagaa atcccgcagg cgctcacgcg
      241 cgaggacgcg gccgcggcca aagccgtagc cgaagagtac ctgaaaatct tcggaccgga
      301 gcgctttttc atcgagcttc aaaaccacgg cttgcccgag cagttggcca tcaacgccga
      361 attggacggc ctggcgcgcc gcctgggcat cgccaccgtc gccaccaacg acgtgcacta
      421 cctgacgcac gacgacgtcg ccgcgcacga cgtcctatgc tgcatctcga cgcgctccaa
      481 ggtcaaagac gagaagcgct tcaaattcga cgccgaccag ttctacctga agtcgcccgc
      541 ggaaatggcg gccgctctgc cggattggcc ggacgccctg gaaaacacgc tgcgcatcgc
      601 cgagatgtgc aacgtcgagt tcgatttctc gcagcgcttc gcgcccaaat tcgagccacc
      661 cgagcgcaag tcgccggatg aatacctgcg cgaactggtc tacgccggcg ccaagacgcg
      721 ttacggccag atcagcgacg agctgcgcga gcgcatcgat tacgagcttg aagtcatcaa
      781 atccaagggc ttcagcggct acttcttgat cgtctgggat ttcgtccgct acgcccgcga
      841 gcaagacatc ctcgcggtcg cgcgcggcag cggctgcagc accgtcgtcg gctactgcct
      901 gggcatcagc gccgtcgacc cgctgcgcta cggcctgtat ttcgagcgct tcatggatcc
      961 cgagcgcgac gaaatgcccg acatggacat cgacttcgcc caggaccgcc gccaggaagt
     1021 catcgactac gtccgccgca agtacggcca cgtggcccaa atcatcacct tcgggcgcct
     1081 caagccgcgg gccgccatcc gcgacgtgtg ccgggcgctc gacgtgccgc tggcggtcgc
     1141 cgaccgggtg gccaaactcg tgcccgaaga gctgaagatg accatcgaca aggcgctcaa
     1201 gcgcgagccc gagctaaagc gcctggtcga tcaggacgac accatccgcc aggtcatcga
     1261 ccacgcgcgc cggctggagg gcctggcgcg gcatgcctcg gtgcacgcgg ccggcgtcgt
     1321 catcgccgac gtaccgctcg acacgctcgt cccgctgcac aaaccggccg acgcggagga
     1381 agtcacgacg cagttcgaag gtccgatggt cgagaaagtc ggcctgctga agatggactt
     1441 cctcggcctg cgaaccttct cacaaattca ccgggcctgc cggctgatcg agcagcacca
     1501 tggcgtgcgc atcgaactcg acaaactcga ccttaccgac cagcgcgttt acgagatgct
     1561 gaccagcggg caaacgcgcg gcgtgttcca gtttgaaggc ggcggaatgc gcgacgtcct
     1621 catgaaaatg aagcccaacc gcatcgagga cctgatcgcc gccaacgccc tcttccgccc
     1681 cggcccgatg gagtacatcg acgaatacgt cgcccgcaag cacggcaaac cctggaccac
     1741 gccgcacccg gtcatgaccg aggtgctcca ggagacctac ggcatcatgg tctaccagga
     1801 gcaggtctcg cgactggtga accgcctggg caacgtcccg ctgcgccgcg ccttccgcct
     1861 cgccaaggcc atctcaaaga aaaaagaagc catgattaac gccgagcgtg agcccttcct
     1921 cgaaggcgcc atcgccaacg gcgtgaaacg cgaagtcgcc gaacacatct tcgccgacat
     1981 cctcaagttc ggcggctacg ccttcaacaa ggcgcattcc accggctacg cggtcgtcgc
     2041 cttccagacc gccttcctca agtgctacta cccggtcgaa ttcatggccg ccctgctgac
     2101 ctatgagagc ggcaataccg acaagatcgc cgagtacctc gatgaatgcc gccgcgtgca
     2161 ccaacccgat ggttcgaccg gtataccggt ccgggccccg gacgtgaacg agagtgaagc
     2221 ggagttcacc gtggcgtacc cggacgccaa aaaggcaaaa aggctaaaag gcacgacgcc
     2281 cccagataaa ccgagcatcg ggcggggcga gattcgtttc ggcctggcag cgatcagcgg
     2341 tatcggtcat aaggccgtgc aggccatctg cgaggctcgc cgcgacggga aattccggga
     2401 tttgttcgat ttctgcgagc gtgtggacct cacggccgtc aacaagtccg tcctcgaagc
     2461 cctcatcaag gccggcgcct tcgacagcac cggtgctatg cgccgcgcgc tgatggacgt
     2521 gctcgaaaaa gccatcgagg ccggccagga tgtgcaacgc gaccggcgta acggccaact
     2581 gagcattttc ggcggtatgg ccgccgccga cgtcccgcgc ccccacatcg gcacccagga
     2641 atggaccgac accgaaatgc tcgcctatga gaaggccacg ctcggctttt acatcaccaa
     2701 gcacccgctc gcgcagtacg aggagctggt caagtcgctc agctctgtcg atacatcggg
     2761 gcttagcgct cttgccgaac gctatggccg tggcaataac aacggcgggc gcaacaaggg
     2821 cccgaccgtc gtcatcggcg ggctgctttc gaaaatccgc ccggtagcca ttcgccaggg
     2881 ccgcagcgcg ggacagaaga tgctggtgct gacaatcgag gacttcgtgg gctcggttga
     2941 ggcgattgtc ttcccggaca tgctggatcg cgtgcgcgaa ctgctcaaac ccgactcggt
     3001 gctgtttttc gtaggcgagt tggacacccg tcgcgaggag ccgtgcatcc gcctcaatcg
     3061 cgtgatcccg gtcaacgaag cccgccgcac gttgacgaac cggctggtcg ttagcatccg
     3121 cagcatgatc gcggacgatg tgctgccgca gttgcaacag gcctgcgccg ctcatcgcgg
     3181 acgctgcccg ctgtttttcg aagtagatac gcgaaacggc gagcgggttg tcatccaggc
     3241 cggagagcgc gcggcggtag actccagcga agaactgatc ctggagctcg gccggctcgt
     3301 cggaccggac aacgtgcgtt gcgtgggccc cagcgggcgc aattgatccg gcagccgccg
     3361 ccctcttaac ccgtcatggc gctgcgtacg ccgtctttac aggccggtcc acttcgtgcc
     3421 ttcgggcagg tcgcgcagct cgatgcccag cgccgtcagg cgcgcgcgaa tctggtccga
     3481 aagctggaag ttcttgtttc ggcgagcttc gttacgcaga tcgaccagaa tctgcatcac
     3541 gtcgttgagc cgcgactgag ccgccgcggg cgtttccggc cacttcagac cgagggcgtc
     3601 ctgcgtcagg cggcgcatga ggaggtcggc cgcggtgaga tcttcgcgcc cagcgccttc
     3661 gcggatccac gtaccggtct gccgggcgaa ttcgaagacc gttgcgatgg cggccgccgt
     3721 gttgaaatcc tcgttcattg cttcggcgaa gctcttctcg gtctgctcga gcgccgcgcg
     3781 aatcgcgtcc gcgcgtggct tggccggagc tgccgcggcg gcctggctta gttcgcgggc
     3841 cgcgtcgcgc agcttgtaag acccgctttc ggccccgcgc aacgcctcgt tcgagaagtc
     3901 cagcggagcg cggtaatggc tggtcaggat gaagtgccgg atcacgaccg gctcgaacgt
     3961 cttctccagc agcggatgcc cttcgtagaa gagctgcttc aggctgatcg cgttgcccag
     4021 gctcttgccc atcttctgcc cgttcagcgt gaccatgttg ttgtgcagcc aatagcggca
     4081 gaactgcttg ccggtggcgc actcgccttg cgcgatctcg cattcgtggt gcgggaactg
     4141 gttctcgatc ccgccgccgt gaatgtcgag cgtctcgccg atgtacttct ggctcatgac
     4201 cgagcactcg aggtgccagc cggggaagcc ttcgccccac gggctcggcc agcgcatgat
     4261 gtgacccggc tcggcccgct tccacaacgc gaagtccgcc ggatgtttct tctcctggtt
     4321 gacctcgacc cgcgccccgg cttccagctc cgacacgtcg cgcccgctga gccggccgta
     4381 cttggaaaat gactgcacgt cgaagtagac cgagccgttg acctcgtagg cgcagccttt
     4441 ctccagcagc ttgcgcacgc actcgatctg ctccgggatg tggccgctgg cccgtgggct
     4501 gatgtccggg cggaggatgt tcagggcgtc catgtcgtcg aagtagctgc gcatgaacag
     4561 ctcaacgacg gccatcgggt gcaggccgcg gcggcgcgct tcggcgatga tcttgtcctc
     4621 gccctcgtcc gcgtcgtcgg tcaggtggcc gacgtcggtg atgttctgga cgtaattcac
     4681 ctcgaagccg gaaaaacgca gccagcgcaa aatggcgtcg aacgaaacgt agctcttggc
     4741 gtgcccaagg tggctgtggc cgtaaacggt tgggccgcag acgtacatcc cgacgcgcgg
     4801 cgggttaagc gggacgaaat cttcggtttt gcgaccgagc gtgttgaata ctcgcagggg
     4861 catgcgatcc gcctttctag aaccacgaag gccgaaaacc agattttacg gcggagcccc
     4921 agcgacagcg agtcgccaga accagatccg agccggcagc cggcgcggtt ccagccgcgc
     4981 gaattcgccc ccgcccgcta caactcctgg ttcttggcgg tcttagcgct ctttgcgtct
     5041 tggcggtcgc ctcggctcag tgcatcacga agccggcgac ggtgtcagcg gcctgcggcg
     5101 gcagcggctt ggcggcgaaa gcgtagagcg acataccggc ccaaatcgcc aggatgacgc
     5161 ccaccacgct ggcgcccgcc agcacgcgtt tccgcgactt gtcgcccacg gtgatgcccc
     5221 acgtgatgca gaacgtcgcg atgccgttgc agaagtggta aatcaccgcc agcgtgccga
     5281 tcgcgtagat cgcgatccaa acccccaacg ggaagacgac gcggaagcct tgccacgtca
     5341 gcagatagta tttcagctca cctggggcct cgctgtagac cggtccgccg aatagatacg
     5401 cgaagcggaa gtgcagcacg tgcgccagga tgaacaccac cgcaatccac gccgtcaccc
     5461 gctgaagcgt gtagcgccaa ttgtccatgt acggatattg gttctggttc ggcttcgact
     5521 ccagggcgat cgccaccccg taaatcgcgt gaaacgccag cggcacgaaa atgaagatca
     5581 cttccagcgc cagcaggaac ggcatctcct gaatccactg cacctgcttg ttgaacgcct
     5641 ccggcccgcg cagggccagc gagttcgtca gcaagtgctc aatcaggaac gcgccgaccg
     5701 ggatgatccc ggtcaacgag tgcagacgcc ggacgagaaa atggttccgg ctcaaccagc
     5761 tttctgctac tgccacgagt tatcccttca ttctcgcgtg ttccgggcta cgccgaaacc
     5821 aaaatgtcaa ttatatgagc ggccccggca cgagcaagca ccgcggagct tgtcccgcgt
     5881 cccacgcctg cttagattga tcggagagcc gcgcagaacc agggccgagc tggcccgcgt
     5941 cgggtcgcgg attcgaccgc agcccatcgg cgtgtcagac tctgatgccg aagccgtttg
     6001 cgcctggccg ggaaagtctc gcctcatgcc caaacgcaac caaccccgct cgttgaagga
     6061 accgcgagcc accacgcccg cgcctgttca tccggccgct gccgccggcg cctttgctca
     6121 cgaatcgtcc tggtcagccg cgcttaactc gcggcgcctg cacgccatcg ctgtgccagg
     6181 cctgctcgcg atcaccgccg ggctggccat cgcctcgctg gtgggcgaca gcgtcacctt
     6241 cgatgaaacc agccatttgc ccgccggcat gagctacctc caaaccggcg atttccgcct
     6301 cgcacccgac catccaccgc tggcaaaaat gtgggccgcc ctgccgctgc tgcttgtcga
     6361 tcatcgctgg ccggcccccg acgattccag ttggcaactc gcctttcact atgacttcgg
     6421 caagcagtgg ctgttcaagc tgaaccaccc cgaccgcctc ataacacccg cgcgcctcat
     6481 gatggtcctc ctgcttctgg cgacatgcct gaccatctat gccctcgcac gcacgctctt
     6541 cgggccaccc gcggcgctgc tggcactcct cctggccgcc ttcagtccca ccctgctcgc
     6601 ccatggccgg ctggttacta cggatatgcc ggtagctctg gcggccgccc tggtcttgct
     6661 caccttcgcc cggctgttac aacgcatgga ctggccgcgc ctgctggccg ccgcgggcgc
     6721 cgtggctatg ttgacgctgg tcaagttctc ctggccggtg gtgctgatcg gcccgggcgt
     6781 catgattgtc ctggcgctgg cgcgaaacgc gccgttggaa tccaggttgc tgcgccacgc
     6841 accgcgcggc gcggcggacg tgcatttgct ccgcacccgc cgggcgcgcg ccggcggcat
     6901 cgcactggtg tgcctgttca ttctcgccac gacctgggcc gccatttgga cctgttacgg
     6961 ctggcgcttc gagataatcc ccagcgcacc atccgccgcg tcgggtgtca tcgctgagga
     7021 caacgcggcg gtggcgcaag tctaccgtgc ggactggcac acaatgctca atgagcccgg
     7081 cggcgcactc aaacgcgccc ggttgacgct cagcgaatgg gtccgctttc accgcctcct
     7141 gccggaagcc tacctgtacg gcttcaacaa cgccatgcgc agcgccgaag cacgcgacac
     7201 ctacttcatg ggccagcact cgcacgtcgg ccgccccgct tactttcccc tcgcattcgc
     7261 catcaagacc cccattgcaa acatcctgct ggtggccgcc ggaatcctgg ctgtaatctg
     7321 caaacccgac ctgcggcgac gcgacccgat cctgctctgc ggcctggccg cgttcggcgc
     7381 cgcctatacc gccgccgccc tgaccagcaa gattaacatc gggcatcgtc acctgttgcc
     7441 gttgtacccg ctgctgttcg tcctgggcgg agcggccgta gcctggctaa gcaggccgcg
     7501 cctgcgctgg gccattccgg gggcgtgcgt atggctctgc ggggccacct tgtggattca
     7561 cccacattat ctcgcgtatt tcaacgagct gatcggcgga ccggccaacg gtcacaagta
     7621 cctcctcgac agcaacattg actgggggca ggacctcaag cgcctggccc aatacgccca
     7681 gcagcacgcc gatcagccgc tcaaactggc gtacttcggc accgccgacc cggccgccta
     7741 cggcatccaa tgcaccgctc tgcccagctt gcgccagtac gacgcggccc cggctgagct
     7801 gacgggcggc gtgtacgcct gcagcgtgaa ccaactcttc ggcctgagcc agcccaaggt
     7861 gcgggatgaa ttctgggccg acccgcaagt gcaacgccgt taccgcgaac tcgtcgcggc
     7921 ggccgccgcc ccgccgccgg gcgaatcctc ggacgcgctc caggaatggc agggcatgcg
     7981 tgaggattac gatcgcgccc gccgctggcg gctgctgaac caactccggc atctgcgacc
     8041 ggacgaccgc gtcggctatt cccttttcat ctacaatctg acgcagtcac aagtagatga
     8101 gctgacgcgg ctctgatcgg cagggatcaa gaacccagag tcgatccggc catcccgctt
     8161 ctggcgggtg tgcaagcgcg gcgctgcgcc gcggtttgcg gagcccggca catgctgccg
     8221 cagcgcgggg ctttcggtaa gctattaact acgcgaatcg gcggcccgcc ccaattcgag
     8281 cccgaagcgc cagcgagggc aaaacctcgc ggagctttgc ccggcaggac gtgccgccga
     8341 aactcgcccg cggttaagat tggcgcgcgc gaaaacgccc gctcgccggg aggctcgccc
     8401 ctgcggttca ggaggttcgc tcccgttgcg tgcggtcgaa cgcgccgctc gcaggagcac
     8461 gtccatgtgg gatgtgacaa cggatattct gatcattctg gctcttatcg tgctaaacgg
     8521 cgtgttcgcc atgtccgaga tcgcaatcgt ctcggcccgc aagacccgcc tgcagtcacg
     8581 cgccagcaag ggcgatgcca aggccggcgt cgcgctggag ctggccggcg cccccgacta
     8641 cttcctggca accgtgcaag tcggcgtgac cctggtcggc atcctggccg gtgcattcgg
     8701 cggggcccag ttgggagacc gcctggccga cagcctggcc ggggtgccct ggctggctca
     8761 gcaccggcag gcggtggccc tgggcatagt ggtggccgcc attacttacg tctcgctggt
     8821 gatcggcgaa ctggtcccca aaaacatcgc cctggcgcat cccgaggcga ttgcacgagc
     8881 cgtggcacgc ccgatgaaat ggctgtcacg tatcggctac ccggccatcg ccttgctgcg
     8941 cggctcgagc aatgcggtga tgcggctgct gcgcatgcgg gccgtcgccc cggtgccggt
     9001 ctccgaggaa gagttcaaga ttttgctgga ccagggggcg cgcgccggcg tatttgagcg
     9061 ggtggagaag acgctggtag agcgcgtgtt ccgcctgggc gaccggcggg tcggctcgct
     9121 catgacgccg cgcaccgaaa ttgtctgggt cgatgccaac gggccgatcg gcgaaagcat
     9181 gcgtactatg tcggccggcg ggcatacgta ctatccgctc tgccaaggca acattgacaa
     9241 cgtaatgggc gtggtgtcag taaagagcca gtgggcgcgg atggtgcacc gcatgcctcc
     9301 ggacctgcgc gaggggctcc tgaccgtgcc cttcgtgccc gaagctatgc ccgctctgaa
     9361 gctcctggag acctatcgtc aggccgggcg gcacgtggcg atcgtggttg acgaatacgg
     9421 cggcgtggcc ggcctggtga cgctggcgga cgtcatggaa gccctggtgg gcgatattcc
     9481 ctcgggcgag gcgcgcgatg acacggccgt cgtgacgaga gaggacggct cgcacctgat
     9541 ggacggcatg ttggcggtcg aggaaatgca agacatcctg cacctcaccg agttgcccgg
     9601 cgaggcgggc gaataccaga ccgtagccgg tttctgcatg taccagctcg gccgcattcc
     9661 gcgcatggcg gaccattttg agtggggcgg ctaccggttt gaagtggtgg atatggacgg
     9721 gcaccgcgtg gaccgcgtgc tggtctcgcc ggccgggacg agcgccccgc aagcagccaa
     9781 ggaaagctga attcagaacg ccggctaacg agccgtgcgg cgcgccgtcc tcatcgcgcg
     9841 cgccgcgtta ccagcgtgca gcttcgctgc tattcgcagt ggctggccac gatgtaacga
     9901 ttgtcgtcgt ccgccagttg cgtcgcgata agtcccggct ggccatccac cagtccactt
     9961 gtcgcccaga ccgtgatgca atcattacgg tccaggtcca ttttgcgcag acagcgcttc
    10021 aggcccaagt cgaccggaac cacgcgcccg tcgtccagac gtaggtcgac caccaggcga
    10081 ctgtcccggt agccgggaat atcttcggtg cgcttggcca caattcgccc gtgatacatc
    10141 tgcagcggcg ccgcggccgc ggtttcaact gcgccgacca actgcggcat ggacccgaca
    10201 taaatcacct ggccgcgcac cgcgagttgg tcggccacca atatcggctg accgtccacg
    10261 aagaggcgct ggccggtgac actgaccaac tctccggttc ccaggcccaa gtaatccagc
    10321 ccggcggtcg ggccgagatc ggcccgcatg acgtaaccgg cgtccgtcgt caagtccgcc
    10381 accaggtgca actgtcctcc gatgaattgc gtctgcaagc ctacgatttg tccttggacg
    10441 gtctcgaccg gcgagacggg ggccgggccg aaaggagccg tccgcaccgg ctcggtgctg
    10501 aacaccagcg ctgaaggctg ctccaccaca acctcggccg gctgttgctc ctggatgcgc
    10561 acgttctgtg cgtacagccg gctgccgctg ggcgtctgct ccacgaagcc ctcggcgata
    10621 aacggcgtac ccggccgcaa gctaaagaag cggttgccaa cctgatcggt cgagcccagt
    10681 acgacgtcca ccagatcgcc ggagtgcgtg cgtacctggg cgaccaacat cttctcgaaa
    10741 tcagcctgtc ccggaatggt atacaaattc accagtgccc cgcgcacctg ccgattagtt
    10801 gcggctaccg gcagcgccag catgctgcgc gccgttacga actgaatggg aacgaatatg
    10861 ttgcctcccg cggcgccctg aatatttccg cgcagcacaa tttgctggtt agtatgcaaa
    10921 tcagggacag tccggaatac gaacgttgcg gcgcccaaat cgagcgtccg gaccgcgccg
    10981 tcctcggtgt gcacggtcac gaaaacctgg ggcggagcaa agtcgctgat ccgccgctgc
    11041 tcaatgttca agatggtgcc gcgcagttcc gtgggcatag gcgcggttac agctacaacg
    11101 ggtgcagtta cggtcgcaac gggcccgctc acggccccta ccgttcgagt ttcgccgccc
    11161 gccagcgcga actcacgcgt taccgtacgc ggctggatgt tgccgggctg aaaccagccg
    11221 tatttccagg actcggccgt cgggtcccag aaccagccat gctcgaatcg gcccgactca
    11281 tccggtatcc acagcttgga ttctgaagtc tggcccgcgg cgggcagtgt cagggcccag
    11341 cccgctacga ttacgccaca gatcgcacag tttgtcagca gcctcacgag ggttctcctt
    11401 cgacagttac gaatactgca ctgccggcat ccgcccggca gagcgcttgc cgccattcgc
    11461 acgctcttaa ctacttttgc ggtccgaaag tgaatacctg atgacaggcg ggttaaaacc
    11521 gacttaaagc agcatattcg aggtttgacc gccttgatac gggcctgcgc tgcattggct
    11581 tctattattc tgatgtcttg aagtagccag tgaagagagg cagtctgaag tgcctccaag
    11641 gtcggctaaa ctggcgccaa acagtcaact cggagctgaa tatgtgcaag ctgtgcgttg
    11701 gaacaagctt tcttttgatg acctgcctgg taattaaccc agcctatgcc gccgacaagg
    11761 agaacgaggc tgacaaggcc tttatgacga aggtggcgca aggcaaccta tttgaaattc
    11821 gcgcggggga actggcgcac aagaagacca cgagcgagga tgttcgcaag ctggccaccc
    11881 ggctgatagt ggatcacact gccaacaatg tcgagctgaa gaagctggcg actaagcaca
    11941 atgtcgagct gcccaatgag ctcaacgaac agcagcgcaa ggagctggcg cgtctggaaa
    12001 agctcagcgg ccctgagttc gagcaggcct acgtggaact gatgatcaaa gaccacaagg
    12061 cggacatcgc ggcgtttgaa aaacaaatcg ccgccagtca gggtgatgtc aagcaattcg
    12121 cggaaaaaac gctgcccgtg ctgcgcgagc acctgaaaat ggcggaagag acgcagaaaa
    12181 agctgaagta atccttcgcc gacgcctccg ccacccgaaa ggaacttaga ggccgaatcg
    12241 gaaggcgggc gaagggattt tctccccctc ccgcccccgg tgcgccgaac ggcgcgccac
    12301 acgcgcgcgc tccccgcggc aaacgcgccc gagagcgggg agcgacgccg gtccgatatt
    12361 cacccgccat cccaccggca aaaattccgc gcgccgtaac tctgaccgcc gcgccgcgcc
    12421 cggttgcctc acccggcatt tccggttctt attagtgttt aaggagtgtg gctatgccgg
    12481 tggattttgg actcacgctc gccccttgga gtttcacgcg taccgcaggc aacctgctcg
    12541 accgcatcgt cggtgaagtc ggcatcgatc atctgaccgt accagccgtc accggcccca
    12601 tggttcagta tcgcctgggc aaggagtttc aggagcctta tttctatacc gaaggcggat
    12661 ggcatttttc gccacgaacc aagctatatg ccgcggtcag cgcccgcccg cacgtggccc
    12721 gttgggccgg cagccgggac acgctcgggc cgatgcgcga gtacgctgcc aaacacgccc
    12781 tgaaattcgt ggtgcgcctc aattttcggg gcttccgagg accgctcatg gctctgccgg
    12841 agacccgcat gcgaacggca tggggcggcg aactcgccgg ctacggcgcc tgcaccagca
    12901 gcccggtttt tcgtgagctg gttcaggcca cgctggcgga cctgaccgcc tatgagccgg
    12961 atggcttcga gctggaccga attcgatccg gcgaggagga attgccgctc ggcgtctcgc
    13021 ggccactgct ctgggatggg ctgctcaagt attgggtacg cacctgtttc tgtccggcat
    13081 gccggcagat cggaagtcgg gcgggagtcg acgtcgatgc ggccgcggag gcggtgcaga
    13141 gtttcggcca agactatgcg cgcaccggat tcaccgcagc gttgggaaag aagctggagc
    13201 accttgggcc atactgggac gcgcgccaca ccgacctgca tgattggatc gaacagattg
    13261 cgcgaaattt cgccccgcgg acggtatacg tcgtgagcga gccggtcggg cccggactag
    13321 tcactcggct cgagcgggac gaacggttcg acgcgcgtga tgcaagcggc ccggatcagt
    13381 cgccggagcc gcctgcgccg gtcagcttca acggcggcag ttttgcccct tacgcaagca
    13441 gttgtgagcc ggcgccaatt acgggtctga tttggaatct attcgcgccc cgaagccagg
    13501 cgggtgacgc gctggtccgg tcgctttctc acgcacttca gttaggattc gagttcttcg
    13561 agttcgagcg cgtcgaggaa atgcccgagc aggccgttac ctggctgaag caggccgtcc
    13621 gctacgctcg acgccaaagc taaggcggaa agcatccacg gagacacggg agaagagaag
    13681 tccaaagagg gaaggcaaat gccaagccgg agaaaacgcc tgccgcgcat ctttctatac
    13741 tcttctctgt gtctctgcgt ctctttagtt aactactcgg gctgcggccg gaaggatcgc
    13801 gacaaaacag gcgccggcgg ccccgatgcc cagacggcct acgtgccgcc gccgcccgtc
    13861 aaaccgcagt acacgttcgc cgaaggcctg cgtgagaaac accccgctcc ggcgatgttc
    13921 gtgcaacagt tcctggaaac ctgcctggcg ggcgattacg ccggctaccg cttgctcgcc
    13981 agtcgcagac gggagccgga gagccgcgaa cgattcaccg cgatctatca tggcattcgc
    14041 gggctgcatg tggagtcgat cgagcagcac agcttccccg agttggccga ggaagtctac
    14101 ctggtcgttt gcaaagtcga tttccctcct gacagcaagg taaaactgcg gcgcaagacc
    14161 gacaaagtcg cgatcctcgc gttcaaagag gagggcgagt ggcgtatgct gcccgcgccc
    14221 gcggcgatgc agcccggatc cgaaacaatg tctgagtccg actacgcgcc ggctaccacg
    14281 agcgcgccga cgtatccgtg ggacgaagag ggtccatgag tttacgcgcc aggtatctgc
    14341 tgacggccgc ttcacttctg ttcccggtcc agcgcggatt cgcagatacc cgcaccgaac
    14401 ttccgcccga cctcgaccaa gaactcgcga cgatccagga tttcacctac gacttcgacc
    14461 acccggccta ctaccatctg ctcgcgttcg tgcagaacag tccgcaagcg ccgggcttcg
    14521 ccacgccgcc gatcgtggtg aatgactggc gcgtgctggt tgagcggccg gctgattacc
    14581 gcggcctgcc cgttaccgtc gaaggcgtcg tgggccgaaa caaggatcca tacgtgcatc
    14641 cgcgccatcc cgagctgcgc ccggtttcac aggtggaact ctcgcgcccg gaccaggcgg
    14701 taagttgtac gatcatcttc acccaggacg tcgcggatat tccgatcggc gccacgctgc
    14761 aagccaccgg atacttcgta aagatcaacc gtttcccgcg ccagagcggc gagccagggt
    14821 tatccgcgct gattgtggcg agcgggccga gccaggtcag tcggacggcc gggcgcataa
    14881 gccaaactgg tccggattgg cgttggatgg tgctggcggt tctggcgggg ctcatcatca
    14941 cggtgtttat gctccgccgc gctcgcggct tccgccgcca caacctgcgt gagctccacg
    15001 ccgatcgcga gccgggcatc aacctcgcgg ctgacctggc ggattgggcc aaacgtgagc
    15061 cgccggattg cccgccgccg cagaaaccag gcaacgcccc atgatcgtcg acgtccactg
    15121 ccattacacg cttacgcgcc gtgccgcgca gccggctgac ggtgcaacgc cgctcgagcg
    15181 cttcagcttc gagccggaaa tcgaggcgga tggtacgccg gcgctggatt ccttcctggc
    15241 gccgcgcata tttcggcgcc ccatctggag cgtcaccccg cttttggcgg gactaggctg
    15301 gaagccgcgc cccggcccgg attttgatcg caagctgcag gcctggtatg agcggcatct
    15361 actggcagac gggccgatcc agcgggccgt cttgctggcg tttgaccgct atcacgatga
    15421 cgccggccgc gccccgccgc cgcccgagcg gcgcggccag ctcggcagcg acatgtacac
    15481 atctaactcg ctcattcgcg acctgtgccg ccgccatccg aaccgatttg tgttcggggc
    15541 ctcgattcac ccctatcgca agaatgcgct ggcctgtctg gacgaggttt tcgcagccgg
    15601 cgcgtgcctg atgaagtggt taccgctgca tcagaacatc aactgcgaag acccgcggac
    15661 catggccatg ctgcggcact gcgccaagtt aggactgccg ttgctgatgc actatggcga
    15721 agaatttacc ttagcctcgc aacatccgca gttcgtttcg gttcagccgc tgttgaaagt
    15781 gctgcgcgaa ttgcgccgca ccgcagaaat gccgacttgc atcatcgccc atgtcgccac
    15841 gcccgcttta ccatggggcg atcgcggccc gcatgcggcg ctgctggcgg ccctcttgga
    15901 cgaatttgcc gacgccccgc tctatgccga catttccgct ctgacagcct ttagcaagct
    15961 gggctggctg cgccgtatgg cacgcgctca ggaactacac cccaaactcg tttttggcag
    16021 cgacttcccg gtcccagtgg caacgacgct gctgagacca tggcttggac gcgattattc
    16081 caaaatcgcg gccacggcct cgtggccggc tcgggcgatc gagatttacc gacatctggg
    16141 attcaatgag atcgttttcc accgcgccgc catgctgttg ccgaacctgt cgcgcttcgc
    16201 gaccgacccc gccggcgcgc ccggcccggc cgctgcctga ctatcattcc gcagtcagaa
    16261 cggatggacg agtggcatgc gcatcaaacc aacctgcgcc gcaatcctca taggcgccgc
    16321 cttcataggt ggttgccgca acctgcgcta cgagaacact gccgcatact tggacgccga
    16381 tttccagccg agcacgcttt caccgttgca catatacacc cgaaacatgt acttcgtccc
    16441 ggcgcgcgac attctgctgc tcgaccccgt gcgacgcgcg gtatttggca gcccggcctg
    16501 gaacgctgac gggcacgaag ttccggatgg ttcgttctac accaaccgct tgcccgaaga
    16561 gctcacgccc gagcgcgtcg cgcgcggacc ctgtactgag cccccgccgg aaccgccgtt
    16621 tacggtaatc aaaaagaagt cggtcggagc gacggccggg ttcatcggac gcgacgcacg
    16681 cggccgcgag tacctcttca agccggatga ccggtgctat ccggagctgg gcagcggagc
    16741 caatgtcatc gcgtcgcgca tctactgggc cttagggtac cacgtgccgg cggagtatgt
    16801 cgtgacggtc tccggcaccg gcgacccggc cttcgaaggc cgccgggcga gtgcttcggc
    16861 catgatagaa ggcggcaagg ggcacattgc ctttgacgct ctgcgttacc ggcgcgagct
    16921 gcgcgggctc ggcttggccg ccgcctgggt caacgacacc gatcgcaacg atcacaacac
    16981 actggtggtc tttgaacacg gtcacgcccg ggtctatctc gtcgatttca acagcgcgtt
    17041 ggggtcgtgg cagggccgcc cgaaggaatc ttggcgcggc tggcagccgg ccggcgatgt
    17101 gccggcgctt attggcaagg tcatgacgct ggggctctgg gtccgcgagc ccgacccgcg
    17161 ccagccggtc atctcgcccg cggttgggcg attcgaaggc tcgcacttca agccggcgtt
    17221 ctggacgccg caatggccga acaacgccta cgcccacatg accagcgccg accgccgctg
    17281 gattgcgtcc aagatcgcct tgctggagcg gccgcacctg gaggcaatcg tggccgaagc
    17341 ccgatacagc gatcccgcgg acgcgcaata cattgtggac acactgatgc agcggcgtga
    17401 aaaaatcctg cgattcgcgg cgcgctgatg cactgttggc ttcccctggg cgccccaggc
    17461 ggattggccg ccgtcgagac ccgtgacgaa cgccgcgctg ccttctctta cggcttcgct
    17521 ttctgcttcc ccttcttgcc gtccttcttc gcatccccct tcttggcctt ccccttcttc
    17581 tccggcttcg ccttttcatc ggccctgcgc ggctccggcg acttgccggc ggcctcggcc
    17641 ggcgcggctt gctcgggcgc tgccgcagcc tgcgtcgccg gaacactgcc ttcaccggct
    17701 tcggacgctg cgggagcgct ggccgtagcg gcggccggtc cctcaggcgg gacaaccaca
    17761 acctcagtga gatcaaccaa ctccacacct ggcggcgtgt tgaaaacaaa ccgctcgggg
    17821 ctgatctcct gattcaactt gatatccgtg taggtcgcgg tcataaccgc tttgccggct
    17881 gcgtcgaaat gaacgatctt taccggatag ccgcagtcct tgcggaaata ttccagcgcg
    17941 ctgccttcgc cgccggagcc cgccttgggc gtcagcttga acacgcagac cgcggccccg
    18001 tcgatggtct cctcgggcag cagttctatg tcgtaaagcg ccttaaggcc ttcgattggg
    18061 tcggcgtccc agtccacctg cgattttttc ttatacgcag tcttctggtc gccgctttcc
    18121 atatacgagt agacgtagcc cgtgccgtcc ataatggcca gcgccatgcc ttcctgcttg
    18181 acggtctgcc cggccgcttc gcttgtaacg acttcccgcg actcgtttcg caggaacact
    18241 ttgccatcct tgcgtagcag ctccagaacg cccttgctga cccgctgctc ggagtatccg
    18301 ggcgagctca tttcggccac cgtctgggtc ttggccgaga gcgatgtgac cgcccgcatc
    18361 ttctccgtca gcatccggct cacctcgtcg accgtctctg cccgcaccaa tgcggcgccg
    18421 ctcaccagca gcgcgcatac gttcagacca cgcagcacgg aacgcatgct cattctggaa
    18481 ctccttgttt gcccgaacag cacctcgcag gcgccacaca caactttata gcgaattaac
    18541 cgccggaacg caccctcgtg tgcggcgcga tttagcggct ttcatcccga gattttggag
    18601 tggccgtgcg caaaataccg tccaggtcgc gctgctgttc tggcgccgcc tcgccaagag
    18661 acagcccgcc tcgcaatttg ccgtagtcgt gatgttctgg gtggagcagg gcgagcggct
    18721 gctgaatagt ccgcacgtga atgtcacgtt gcggcaaggc gatttcgatg cccgcctgcc
    18781 ggaattcgtg gtcaatggct ttgttcaaat cgtgccgcgt gctcatcgac aagtcgacgg
    18841 aagccacgaa gacgcgcagc tcaaactgca atgcactgtt gccgaaaccc atgaagtaca
    18901 cgcggggcgg cggatcggac agcacgttct cgtgcgcgcg tgccactttg taaagcagtt
    18961 gctccgccag ctccgtatcc gactggtacg cgatcccgac cggaatgatg atgcgcagcg
    19021 tggagtcgga cagtgtccag ttgatcacct tgccggtaat aaactcctta ttggggatga
    19081 ccaactcctt gcggtcccaa tcggttatcg tggtcgcccg gatgcgaatc cgcgagacta
    19141 cgccgctgat atcgccaatc gtcaccgtgt cgccgatgcg gatcggccgc tcaaacagca
    19201 gcatcaagcc cgacacgaaa ttcgcgaaga tctcctgaag cccaaagccc aggccgaccg
    19261 tcatggccgc caccagccac tggacccggg accagcccac gccgatctcc ccaaacgcaa
    19321 tgaccacgcc cgccacaatg atgaggtagc gggctaccgc ggtaaccgcg aagcgccccc
    19381 cagtatccag cggcaaacgc tgcaaaatca caatttcgag caaacccggg atattccggg
    19441 ccagcaccaa agtggccaaa gccaccaacg ccgcggtcgt cacgtctccc agagtcacgt
    19501 acttgatgac ccgcgccgca gcgccgccca cggccgccac gccctccgcg ctctgcccgg
    19561 ccgcttcagt gcccgtgtaa gaccagagct tggtctcgcc tacgacgctc agggccggca
    19621 gcagttccga ccagcagagc cacagtccga tggccataat gaagatcacc accgctcgca
    19681 gcagcttgcg cgtctgcgcg ccgatgctga tcaggtccag ctcctcgtcc tctaccgccg
    19741 gcgcctcttc gtgcgcggcc ccttgctcgg ccagccgggc cgccgccgcg gcgcgcttgc
    19801 gccgcgcccg ttcgtgcgcc aggcggcgct gggcaacgaa gaacgcgcgg cgcagcaacg
    19861 cgtgtatcaa caacacaatc agcgccagcc gggccgtttg ccccagccgg tgcgccattt
    19921 cctgggcggt gtagtagtag ccgatcgccg cgcccaccgc cgacagcagc ggcaacatca
    19981 gcagcgcgac cagcagcacg tagcgcgaac gataaagcca cggactgtga tgccgcagcc
    20041 aatcaacgaa aatgccgcgc tctggatgcc cgattcgcca caagaaccgg atgacggccg
    20101 ccataagcag caggaacgcc gaccggccga tcagatcgcg cacctcggcg tccgggtaaa
    20161 cctccatggc ggcgatcacg aacagggccg gggcggcgat cgccatcagc gagagcgcgc
    20221 tgcgccgtgc tacccgcagc cccgcggcat tccaccggaa atggtactcg cccaaaccgc
    20281 cgggccggca aatctcgcgc accagcgacg taaagaaaat gaccattccg gccgaaatga
    20341 ggcccgcgcc gagcgtctgg gccatttcgt aggccgccac atccctggct tccgccgcgg
    20401 ctctgacggt ccaggcggcg aaccacagcg cactcggcca gcacgcggct cgcaggatcg
    20461 agcacagcag cgccaccagc gtgtggcgga aagaatccgt gtggattttc gccacgcgca
    20521 aacccgtgtc gcgcactttg gcggccagca gcggccgcac gcttagccac gccagcagca
    20581 ccaccagggc caggccggat acaattaggc ctctgaggcg ctgttcgggc tgctccggca
    20641 gcagcagtcg ccgcagcgcg ccaagacgcc gcgtcgcacc ctcccgatcg tctggccact
    20701 ccgccttgaa gattggcgcg gtgctgggca gccagagcga atagcggtca acgacttcac
    20761 gcgttgcgcg cacgctgttc acaatgcggg tctgtacatc caccaattgc agcagttcgg
    20821 tggaatagct ttcgtagtcg gtccgcagac cctccagcag ctcgcgccgc gtttccaaca
    20881 tctcacgaat cttgggctcc agcggcgcca gatcgatgtt cggatcatct tcgcgcagct
    20941 tcgccagcat cacgcgcttg gcggcgtcca catccgtcag aaccagcagg cgctcgcgca
    21001 cctcgctcag tcgactctgg acctgcgcgt acttcttgcg agtttcaagc gattcccgct
    21061 ccgcatcacg cagccggtaa aggtcgagcc gcgccccgcg catcagcggg cccagcaggt
    21121 cgcggtccga atcctgctcg acgcgctgcc gcaaccgccc caaccgggcg ctgatgcgtt
    21181 cttcatccac acgcgccttt tcgagcttcg cctgcgcctc ggccaagccg gcgctgacgt
    21241 tctcccgctg cgccgccaga cgcgggttga tttcaagttg cctttgaatt tcccaaggcg
    21301 cgtccttcag cccctccgcg gcgcgctgct tctgcttctg tacttccgcc gcgcgcagct
    21361 cagtcgcttt ggcctgtagc tcgtccagca gcgcttgcgc ctgcgccacc tccaagcggg
    21421 ccttgcttcg cctggtcgcc aacacctcgc tgcgcgcctg gtagaacgcc aattcctcct
    21481 gatacgccct gatctcctgc tcaatagcgc tgcgcctggc gagcagcaaa aaacgttgcg
    21541 ccagcgcaag ctgaggaggg acgcccgcct cggccgcccc ggccaattcg gcactcaatg
    21601 tttcaagctg ttgctgggcc gccgtacgca gggccggtat ggcttgggca cgctccgcgc
    21661 gattctgcgc ctgggccgcc agcttgtcgg ccgcggcttg tttctgacgc agttcgctgt
    21721 cggcgctagc gattttctga ccaagatcct ccagagttgt gctctcggct aactggagct
    21781 tgacggccgg agcgttggtt accgcatcca actcctgctg aattgcctcg agatcggccg
    21841 gcgcgttgct ctgaccttgc tgatattcgg agattttgga gctccaggtt tgcgcgagcg
    21901 caatttgctc cagcacctgg cgataaatct ccagaatttg ggcgcgcatc ggctccggaa
    21961 gttctgtgga ggcctcgatg ctggccatgc gcgcctgaat ttcttcaggc gtgatcgtgg
    22021 ccaccgactc ctcgtgattg gccgacgctt gggttgtcga gggctgcgac gaagccggcg
    22081 ccgactctgt agccggctga ctggcggttg tagagctgct gacggccgtt tggactacgg
    22141 gctgcgtctc ggccgggccc aaatccatct ctgtcgccag cagggctgac gtgcaacact
    22201 gccccaatag aagtaatgcg cctagcacgc tggcgcagcg ccggtgttcg tatcccatcg
    22261 ccgttccact ttgttcgcgc tcggagacac ataataacgt caatctaacc tgtcgtcgcg
    22321 ggccgctttt taggaccttg ggcggatgtc caaatccccc cacatcggat acaatcgcgc
    22381 agacgcgtgt aacttctccc ccgacctttc aaaggacccg catgaaagta ctcctggttt
    22441 ccccggctac gccagacacg ttctggagct acaagcacgt cctgccgttc gtagccaaga
    22501 aggccgcttt cccgccgctg ggcctgctga ccgtggccgc tttgctgccg cccgaatggg
    22561 agctcaaagt ggtcgacttg gacgtgcgcg ccctggcgga cgacgacatc gagtgggccg
    22621 actacgtgct gctgtcggca atgatgatcc acgagaaatc ggtgcgcgag gtcgctgccc
    22681 gctgtgccgg ccgcggcaaa gccgtcatcg ccggcgggcc cttgttcacc acctcgcccg
    22741 aacgcttctc cgaaattccg cacctcgtgg tcggcgaagc tgaagagttg atggaccaga
    22801 tatgtcgcga catgctggcc ggcacgctgc gccccctgta tcgctgtcac gaacgcccga
    22861 gtttggcaaa cacgcctttg ccccgctggg atttgatcga cttccgcgat tacgcgacca
    22921 tgtcggtcca gcactctcgc ggctgtccct tcaactgcga attttgcgac atcgtcgaga
    22981 tttatgggcg cgtgccgcgg ctcaagagca cgcagcagat gatcggcgaa ctggatgcgc
    23041 tggtgaacgc cggctggcgt ggcagcgtgt tcatcgtgga cgacaacttc atcggccata
    23101 aaaggcgcgc taaagacttg ctccgggcga tcatcgactg gcgggcgcgg cgtggcgtga
    23161 atctctcatt cacaactgag gcttctctca atctcgttga cgaccccgag ctaatcgacc
    23221 tgctggtgca agccggcgtc aagaaggttt tcatcgggtt ggagacgccc caggaaagca
    23281 gcctggccga gtgcgccaag acgcagaaca tggggcgcga tctggtcgcg gatgtcaaaa
    23341 cccttcaggc cgccggaatc gaggtcctgg gcggcttcat cctgggtttc gataccgact
    23401 ccggaacgat ctttgagcgg caattgcggt tcatccagga atccggagtg gtgaccgcga
    23461 tggtcggcct gctgaccgcc ctgccgcaga cgcgcctgtt cgcgcgcctt aagcaagagg
    23521 gccgcatatt gagcgagagc accggtaaca atgtcgaagc ggtcctgaac tttgtgccca
    23581 agctcgaccg ccaggtgctg gtcgaaggct accgcaatct ggtgaagcac ctttattcgc
    23641 cacagatgta ttacgcccgc gctctgacgt ttctgaggca atatcggccg cgcggcccgc
    23701 gtgccccggt gcgctggtcg gacgtccgtg ccctgcttca ctcgatctgg actatgggcg
    23761 tgtggaccaa cggccgccgc gagtattgga agttcatgct ccgctcgcta ctgtttcacc
    23821 cgcgccagtt cgtcgaagca gccaccctgg ccattctcgg gcatcacttc cggatagtcg
    23881 cagcgcgttt gtgacagtgc cgaagcataa gaagtctgat tgccgccgtg cgactctctc
    23941 gcccgacggc cgcctcctgt cgcgaggccg acggccgccg cttcctcgcc cggagggcgc
    24001 gcgaacgtag ccggggattt caatccccgg tgggaacgcc aacccccccg aaactttgta
    24061 gaataggccg catgagcacg acgctggagc gcgtttggca actggtcgcg accgggcaag
    24121 tcaaggtctc cgaccatggc tatgatgaat tgagcaacga cggattgacc gtgcgcgaag
    24181 tagtggacgg tatcaaggcc ggacaggtcg tggaggacta tccggcgtac ggaaagggcc
    24241 cgtgcgtttt ggtgcttcag cgtgacgaac gcggcgcgcc actccatgcg ctgtggggca
    24301 ttccgcgcgg gcaggacggg ccggctgttc tgatcacggc ctatcggccc gacccggcga
    24361 agtggacgag cgatttcaga gtgaggctgc catgaaccgg cgcacaacga agctcattcg
    24421 cgaaggcgac tacgtgatcg agctggatgt cgagctgagc gactcgcccg acggctgggg
    24481 gccgtacttg tcgctgaccg acgccgagcg cctcgacgaa gcgcgggcgg cgttacggca
    24541 gggtgacctg gccgcggcaa cgcggctcgg ccgcgtctat cggctgacgc cggtcaaggc
    24601 gagcgcataa ggttgggcgg cgatagcatc caaatcgtcc agcgctgctg ccagccagac
    24661 tacttgatga gccatttttc aagccgcttc tcggcctcgc tttgcgggac agactccccg
    24721 cgcgccgccg actcgagccg gcgacgaatt ttttcaatga cgtacaggtg tactgtacgt
    24781 cttcgaccga gcaatcctcg ggcaggcgct tcaatatctc ctgcacttgc cgctttacat
    24841 ttgacatacg cgcacctcgc acgcgcccga gtagagttta gcttgcggcg cgctgtcagt
    24901 ccacgagcgg aaacgtgact ggcaccgagc cgcctctctc gcccgacggc cgcctcctgt
    24961 cgcgggggca acggccgcag cttcctcgcc cggagggcgc gcgaacgtag ccggggattt
    25021 caatccccgg tgcgtaacgc gaacgccccg tttgttttct ctttcctgat ccggtcttcc
    25081 aaaagcgccg tgtgcgattt ccagtacagt tgtaagtctg ccaccgtaaa gatggaatgg
    25141 cattgtcgcc ggccgccttg caacggcagc ccaaagtaga ctgatcggga ccggaagcgg
    25201 taccccggct cggggggggg aggggagtca atgccatgtt ctttaaccct gcggtcggca
    25261 acatcagcga ttgcgtggag gcaacagtct ggtcgcggtc ggtcgacctc atagacggtt
    25321 ccgaattcga ccggcgcgac cggcatcgaa gaacttgggc ttggtgaagc gccgcggttg
    25381 ccctcgcgca gacaagaacc ccggcacgag tgccggggct cggaagctgc ccaatgttat
    25441 gcgggtcgcc acccgtcaca tcttgattcc tcgatcccta aatcccttga tccctcacct
    25501 gcagtatcaa tactccggcg gcaaccggct gcaatccgga gcagtatggc gtgcaaaccc
    25561 acatgccgcg cagtctgtac gaaccgccag ccgctgacaa tccccccgcg tttctgctgt
    25621 ggaaccaatt ctcaaccggt cgacgggtta caaaccgccc acaatacggc ctattccggc
    25681 cgctgcgtat gccggccgcc acctactccg cccggcgcaa ccagagatac acgttggtgt
    25741 tggtgcccga caggcgaaac acgcccaggt cttccacgcg gccgccgtac tgctccatta
    25801 ggtacgcagc cagtggctgg ttcggctgcg tggcggcgat gactgcgaag agttggcttt
    25861 gcgggccgca cgccgcggcg atctcctgtt ccaccgcgtg gatctcctcc tccaagcggt
    25921 ccggctgttc agcccaggca gcctgatcct gactggccat gtgcagcgcc atttcccgcg
    25981 ggtaagagaa cagctttacc ggcacggcca gccggcccag atagtactgc aatggggcgc
    26041 gcgtcaggcc ggtgcaaagg accgcgtcgc cggcccgggc cgcgcgaaca atgatttgtg
    26101 ccattgcacg atcggcgctg gcgagctgat tgccgtacag atgcgccagg ccggcgcacg
    26161 agtagacgca gaacaccacc agcaggcaat agcgcagcag cgccgatgca actgcggcct
    26221 ttcgccagtg caggcccgcg aggccccgcg cgacgagcag cgcaaagcac gggtagacca
    26281 actggtcgac gcgcccgggc acccagttcg ggacgctgat gactgacgtc agcgccgcgc
    26341 acgctagcgg caataccgtc gcacaggcca gccatagccc acgcgggttt accttaagcc
    26401 cgcgcggccc gcgctgccac gcctgcgcga ggccggcggc cgccgccagc gcgcacaagt
    26461 agaccggcgt ccagaacgtg tgctgttccc gtgcgaagag aaagaagggc tgtggatcac
    26521 cgggagaaaa cgagcgcagt gtgctcaacg ggatgcgact tgacccgaag cgttgcgtca
    26581 gagtcgcaaa ccacacgtac tgctcccggt taccgagctg catcaggaac gtcggcagcc
    26641 atggcagata gccggccaga atggccgcgc cgcatgccgc ccagtgccag ggtgcacggc
    26701 gcagcgcgcc cgaggcaagg atgatcagcg catgcacggg cagcaggtgc aaggcgaaat
    26761 tatgcgtgta cagcgctccc agagtggcca gcacgtaggc tacgtagaat cgcccgcggc
    26821 catcctcgat cgcccgccac agccagtagg aagagagcag ccccagcagt cccagcaggg
    26881 aatacatgcg ggcctgctgg caatagaaca catgcgtggg cgcgacggcc gccagaaccg
    26941 ccgcgagcaa gccggtctgt gtgccgaaca accggcggcc aatggcgaag agcgctccca
    27001 ccgtcagcac gccgggaatg acggatagcg tccgaaccgc cgcctccccg tccccaaaca
    27061 ccttcatcca ccccgccagc agcaggtaat acagcggcgg gctcgaatcg cgttttagac
    27121 cgtccagcag tccggcaaca cccgttttgg ccaacaccac gctgttggct tcatcgatcc
    27181 acagatcttt gcccgccaag tcatagacgc gcaccgcaat agctgtgacg agagttgccg
    27241 ccaccagcac ggcgacagca cgtgcctgcc acgtatcttt gccccaccct cgtgatggcg
    27301 tcatgctcgc cgccgtgccg gagactaacc ctgggcgcgc ctttggcatc actgcgagtt
    27361 accgattctc cgccgccaag tcaacccggg ccgcgaagtt aatccgggga tcaatcccgg
    27421 ggtaaatccg gggacgcctt ggaaaatccg aagatccgcg gacgccgttc ctaaaccccc
    27481 gccctgattc tccccattgg tctcgcgccc caaaatcgcg cacacgtcgc cattgctgcg
    27541 catatgctaa caatccctgc ccctgcgccc ctgcgtgcct tcgccctctg cctatacttc
    27601 ctagcgaccg gccacgcccg cgcgcagaaa ggagctctat gtgccgtaca ttcttgctcc
    27661 ccgattccgc atccttgatt cgttgcaccc tgcgggcgga taactctcaa gagtgcaaaa
    27721 ccgggggcac gcaaaatcga actccttgca cccttgaggt cctacccccg ccgaccgcgt
    27781 tattcccgga ccttgccgct tacactctac gctctttgca cccttggctc tacccgcgca
    27841 ccggagttgc cttgctgctt cccctgcaca aacggccgac cacaggtcgg ccgctaccct
    27901 ttgtgcctcg ttgccttgtg gcctcgttgc cttccggcct agtactccgg tggcaattgg
    27961 ctgagctgcg ccatcgtgaa caccggcccg tctttgcaga cgtagacctt gcccacgttg
    28021 cagcggccgc acttgccgac gccgcacttc atgcggtttt cgagcgtggt atagatctgc
    28081 tcgggcttga aattcagctt cgccagcacc ggcatggtca gcttgatcat gatcggcgga
    28141 ccgcaaacga tggcgatcgt gttgtccgca gacggggcaa gctgctccag aatcgtcgga
    28201 acaaagccaa tctttccctt ccaatccggt gtctcgccac ccggatcgac cgtaacggag
    28261 aggttgacgt ccttgcgggt gccccattcg gccaattcgt gcttgtagac caggtcggcc
    28321 accgtgcggg cgccgtagat aatgcttacg tcgccgtact tatcgcgcag gtcaaggcag
    28381 ttccagatga cgcagcgcat cggcggcagg gcgataccgc ccgcgatgaa cagcagcttc
    28441 ttgccgtgcc actgctcgat cgggaacacg ttgccgtacg gcccgcggaa gccgatcacg
    28501 tcgccctcgt cgcggtcggc cagggcggaa gtcacgcgcc cggcctggcg gaacgtgcat
    28561 tcgatgtagc ctttgcgcgt cggcggcgag gcgatgcaga acgtgctctc gccctcaccg
    28621 aacacggaat actcgccgaa ctgcccggcc ttgaaagtga acttctcagc gtcggccgga
    28681 tcgacgaatt ccaggcggaa tgtcttcacg cccggggcct cgtccgtgac cttggcgatg
    28741 cgcatgcggt aggggacgta caggtttgcc ggcttgccgc caggagcagg cgccgcaccg
    28801 ggaggggtgg ccatcttggt catgccttca tctccgccaa agcctgtgca tgttcggcca
    28861 ggttcatatc caccggacac gttcgcgagc agcggccgca accgacgcag cccagcacgc
    28921 ccaaccgttc cggctggtac gagaacttgt gcatcacgcg ctgccgccag cgctcactct
    28981 gcacgtggcg cgggttgtgg ccggaggcgt gcagcgtaaa cagcgagaag ccgcacgaat
    29041 cccagcaccg cagccgcgtg ccgccttcgc ggttgcgctc atcctgaata tcgaagcacg
    29101 agcaggtcgg gcagacgaac gcgcacgccc cgcaaccgag gcagcgcagc gactgctcga
    29161 cccaaaccgc attgttgccg aaggccgccg gcagcttctc cttcagcttg tcggccgaaa
    29221 agcgcggcgc gaccttcgcc agcaccttgt ccttggtctc attgccggcc ggcgtgaaga
    29281 gctgcggggc cgcggccacc accgcctggc ccttgggcgt cagcacctcg accaggaagc
    29341 gctcggcgtc gagcggcgtc agcagcatgt cgctgccgcg cgtgtcgccc gggctgccgc
    29401 cgaccgacgt gcagaaacag tacgcgtcgg ctttggtgca agccaggccg atgatcgtca
    29461 ggttcgccag ccgcgtctcg aactgtgcgt ccgggctgtc ccaggtgaag accgccttga
    29521 gcgtggcgaa gctggccgcg tcgcacggat gcgcgccgaa cagcaccgtg gggcgcttgt
    29581 cgggctcggc cggctgctgc tccacgtcct tgccgttgaa cttgtagcgc agcagcggct
    29641 catagggcgg gaacgtcgcg agcttggccg aactgcgggt ttgaatgtag tccagcgcca
    29701 cttgctccgg cgacttcacc tctgcgaagt tgagcagttc gccttccttc accggcgcca
    29761 gcaggcgttg cccggcggcg gtgagcttcg cgaagagctc gccgagagac ttcttttgta
    29821 gaattcgtgt ttcgctcatt gtctcaccgg atgaactctt ccttgtcgtc aggtttgaag
    29881 gtggacaggg cgtactcctt gcgggtcgag tagccggcct cgccgccgaa gctcgtcagc
    29941 acttcctgag ccagcacgcg gttcagcagg tccagacgga tgccctgcgg gcaagcgtca
    30001 ctgcacgagc cgcaatcgac gcagcgcccg gccaggtgca tcgcccgcac gatgttccat
    30061 tcgaggttgc ccagcggatc ggttgcgacc gggatccact gcggctggtt tacctcggtg
    30121 atgcagcggg cacagtagca catggggcag gccgcgcggc acgcgtagca cttgatgcac
    30181 cgcgacattt cggccgacca gaacgcccag cgctgctcca gcggcatcgc ttgcagccgc
    30241 tcgatctctt tctgcgcctc gggcgacaag ccgcgtggca ccttggccag gtagacctcg
    30301 agatccgcga acttggccag ctccgtgatc tgcccgtcgt cggacacgat cagcagcacg
    30361 gccgcatcgt ccgcgatctg gttctcagcc gcgtattgca tgaccgtgcg ggccgtcgta
    30421 acacgaccga cgaccgccgc cttgcccagc gccttgactt ccggcttcag caggtacgtc
    30481 gcgaggttct gcttgcagct ttcgccccag atgagcttgc ccgcctgctc cggcgtacgt
    30541 gcgaacagcg gccgggcggt ctgcgcgtca gtgccggccg cgaagccgat cacgaccttg
    30601 accgtgccgt tctccagcag cgccttggct ttcttctgta gtgcttccat caggccacct
    30661 cgagcggccg aatctgcccg ctcagccgtt tcgggaacgt gtcgtatggc ccgagcttgc
    30721 gcacgtcggc cacgacgttg ttgaccagct cggcccactt ggcgccctcg gccgccgata
    30781 cccacgagaa gtacacgcgc cgcaggtcga tgcccatgaa gtccagcagg cggcggaaga
    30841 cgatccagcg ccggcgggcg tggaagttgc ccgcggtgta atggcagtcg cccgggtggc
    30901 agccggagac gatgacgccg tcggcgccct gcccgaaggc ctgcaagagc agcatgaaat
    30961 caatgcgccc ggtgcagggc aggcggacca tgcggatatt gggggcgtac ttgatgcggc
    31021 tggtgccggt caggtccgcg cccgcatacg tgcaccagtt gcacacgaag gcgatcactt
    31081 tcggttcata gtctgccatg agtgctcagc tttcagctat cagctttcag ctctcgccat
    31141 tgctcacaac gcgagcagtt cggcgaagat ctgctcgttt gtgaagccct gtaaatctat
    31201 agacttcgac cggcacagcg ccacgcacgt gccgcaaccc tggcagacgc cctcattgat
    31261 gcgtgccacg gtcttgatca gcttgccgtc gcggccgcga atctcctcgc gctcgatggc
    31321 ctgatacgca caagccgatt cgcagaggaa gcagccgatg caggtcgaga acagcggcgg
    31381 ggcacagcgg ttcaccttcg cgaccaccgg ctcacgctgc aattcgtcct tcgagaacag
    31441 ccccgttacc ttgctcgccg cgcccgaggc ttgggccacg gtttcgggaa tgtccttcgg
    31501 cgcctggcaa gcgccggcga caaacacacc ggccgtattc atttcgaccg gccgcagctt
    31561 ggggtgcgcc tcggacagga aatggtcgcc gtcgtagctg accttgagct tttgcgccag
    31621 ttcctcggcg tccttatggg cgaccatggc cgtggccagc acgaccatat ccgcctgaat
    31681 ttcgaccggc cgggagttca gcagcgtgtc gacgcccttc acaatcagct tgccgtcgcg
    31741 ctcgtaaatc ttcgacacgc gtccgcggac gtagtgaact tcgtcctcct cgatcgcgcg
    31801 gcggacgaat tcctcataca tcttgccgcc ggcgcgaatg tccatataga agacatacgc
    31861 cttgccgctg tgcaccttgt gcttgtagag catcgcgtgc ttggcggtgt acatgcagca
    31921 gatcttggag cagtaattga tgcccttcgc cgggtcgcgc gagccggcgc aggcgatgaa
    31981 gacaatcgtc tcgggcgtct tgccgtcgga cggccgcttg attgcgccca gcgtcgggcc
    32041 ggaggcggaa gccagccgct cgaactgcag gccgttgacc acgtccttgt atttgccgta
    32101 gccgtactcg gggaagaagt cggtctgctt gaggtcgtag ccggtggcga cgacgaccgc
    32161 gccgaccgga acctcgatca attcgtcttc ctgttcgaag cgaatggcct tcttctcgca
    32221 gaagcgctcg cacaccttgc acttgccggt gcggaaatac gtgcaatgcg ccttgtctat
    32281 gacgggcttg ttcggcacgg cctgcgggaa cgggacatag atcgcggtcc ggttgcccag
    32341 cccggcattg aactcgctcg gaatcttctt cgtcgcgcac ttctgggcgc agatgccgca
    32401 gccggtgcag agcttctcat cgacgaagcg ggccttcctg cggatggtcg ccttgaagtt
    32461 gccgatgaag ccctcgagct tctccagctc ggagtaagta tacagcgtga tgttcgggtg
    32521 ctgagccacc tcgaccatac gcggcgtcag gatgcactgc gagcagtcca gcgtcgggaa
    32581 ggtctcggaa agctgggcca tgtgcccgcc gatcgacgga tcgcgctcga ccagaatgac
    32641 cttgtgcccg gcattggcga tgtccaggct ggcctgaatg cccgcaatgc cgccgccgat
    32701 gaccagggcc gtcttcgtga cgggcacctt gatcgggaac agcggccgat tgcgcttgac
    32761 cttctcgacc atgatgcgaa ccaggtcgat ggccttctcg gtcgtcgccg gcagcttctc
    32821 gtgcacccac gagcaatgct cgcgcacgtt cgccatttcg cacaggaacg cgttcagccc
    32881 ggcgtccgcg caggtgcggc ggaacgtcgg ctcgtgcata cgcggtgaac aggccgccac
    32941 cacgacgccg gtcagcttct tctcgcggat cgcgtcgcgg atcaggttct gcccggggtc
    33001 cgagcacatg tacttgtaat caacgctgtg caccacgccc gggaaaccgg ccgccgcctg
    33061 ggcgaccgcg gcggcatcga ccgtggcggc gatgttctct ccgcaatggc agacaaaaac
    33121 accaatgcgc ggcatggtct agcctctctt ctctgctgtg ccggcctggg ccggtgcggc
    33181 ggcactcgcg gcggccgccg gcgcaggcgt ggaaatagtc tgtgctgccc gctcgaagcg
    33241 caccggaacg tgatggcgct gcatgcccag cttgtcgggg ctcagcccca tggtcaggcc
    33301 gatgagctgg ctgatgtaaa tcacgggaat cgaatattgc tggttgcggt attgctgaat
    33361 cgcgtcgcgc cgcaggtcga ggttcgtgtg gcacatcggg caggccacca cgatcgcctc
    33421 ggccccgcgc ctgaccgcgt catccacgat ctgccccgac aatcgggcga ccgagtccag
    33481 cttggccacc gagagccccg ccccgcagca ctcgaccttg aacggccaat caatcggctc
    33541 ggccccgcag accttgacga tgtcgtccat ttcctgcgga tcttcagccc gcttacaggt
    33601 gttcagcgcc gccggccgcg tcaggtagca gccgtaatag caggccacct tgcgcccgaa
    33661 cggcttgctc accttctctt tcaggccgtt cgccgccgcc cggctgagca cctcgaccac
    33721 gttcaggacg cgggtggtgc cgttgtaagg cagctcgatg atgcggctga tctcgcgccg
    33781 cagattctcg tccttcgaaa gctcgtaccg cgtgccggcc aggcgcgtga agcaggccga
    33841 gcaggggacc agcagctcag aaagcccggc ccgctcggcc agggcgagaa tgcgggcggg
    33901 cagcgccaaa gccagcttgt ggttcaggct atgagcggcg ctggccccgc agcagttcca
    33961 gtccgggact tcggtcagct cgagccccag cagcggggcg atggccgtca gcgactcgcc
    34021 gtattcacgc gaggagccca gcagcgagca accgggaaaa tagccgattt tcatctctta
    34081 tccgcctcgt cgtgatggcc cgtggtttgg ccggcatgcc cgctggcggc ggcgcagcgg
    34141 tcgaacatgg cggcgatcgc gtcgcggccc ttgatcttgt gcggcaggat tttcagcttg
    34201 ccgcgcatga gcagcttggg cgcgacattg acatcctgaa acaggtgcat ggttcgcatt
    34261 ttgtaagccg aaatcagccc gacctcgtgc agccgcccgt tcttcttgat ggaatcgagg
    34321 aacgagcggt ggaaggcgag gatgtccttg gccttcgggt ttgccacgcc gcgccgcagg
    34381 gcctcttccc gcaagtagtc catgatcttc gggatactga cttcctgcgg gcaacgcgtc
    34441 gcgcacgtct cgcaggccag gcacagccag atggactccg ccttcagcgc ctcttcttcc
    34501 agcgccggca ggccaagctg cagcacccgc aggacctggt tcggcgcgag gtccatctcg
    34561 gggcccaacg ggcagcccgc cgagcacttg ccgcactggt agcagttaaa gacggattcg
    34621 cccgtggccg ccttaacttg cccggccagg ctgtggtcat gttcgtgatg agacgaacca
    34681 gacatactct tctccgaagg gctggatcgc aacaacccgg ccgccagccg ggaccgtggc
    34741 tgcccggcgc cgcacgagtc cggtaaagcg ccggccgccg ggtgccatgc ccaagctgta
    34801 agcgcgggca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctaccaa
    34921 gtcgcccgaa caaggggtag actacgcctc tttcggcccg ggggccgccc cggagccgtg
    34981 ggatggttgg cgagggatcc agagatcaaa cgatctaggg gtctttctgc aaagacgaca
    35041 aaccgggata caagagggag ttgagcgatg gcgatcaatc tgcgcgggcg aagtctgctt
    35101 tcgatgaagg acctgacggg ggaggagatc aatgcgatcc tggcgcgggc cgcggagctc
    35161 aaggccgacc gggccgccac gtttcgcgcg gcgccgctgg ccggcaagac gctggccatg
    35221 atcttccaga agccgtcgct ccggacgcgc gtctcgttcg agaccggcat gacgctgctt
    35281 ggcgggcacg ccatctacct ggccccgtcc gacattaaac tcggcgaacg cgaaacgacc
    35341 gaagacatcg cgcttgtgct gtcgcgcttc gccgacatca tcatggcccg cgtcttcggc
    35401 caccagatcg tcgtcgattt ggccaagcac gccaccgtgc cggtcatcaa cggcctcagc
    35461 gacttcgagc acccctgcca gattctggcc gacctgctga cgattcagga acgcaagggc
    35521 cggctgcggg gcctcagcgc gacgtacatc ggcgacggca acaacgtcgc gcattcactg
    35581 atgtacggcg cggcccgcgt gggaatgaac atgaccgtac ttacgccgcc gggcttcgag
    35641 ccgctgccgc aggtgctcga agaatcgcaa gccatcgggc gcgaaaccgg cgcgacgatc
    35701 cgcgtgacga atgatctggc cgacggggct aaaggcgcgg acgtgctgta tacggacgtc
    35761 tgggcgtcca tgggtcagga agccgaagcc gaagctcgcc gcaagaagtt cgccggcttc
    35821 cagatcaaca gcaagctgat gagctacgcc aagccggacg cgatcatcct gcattgcctg
    35881 ccggcccact acggcgagga gatcgattac gccacgtcgc gcacgcccaa ctcggccatt
    35941 ttcgatcagg ccgagaatcg catgcacgcc caaaacgcgc tgatggtgat gctggcgtaa
    36001 agaactgaag aagtgaatgc gagtagtgga tagtcagggc gccccccgcg actatccact
    36061 attcactttt acgattattc acttttacga tccactcttt cctgcgcgaa tagccagcgc
    36121 cacaggtccg ggtcgtcgta cgccttgtcc cagacgttgt ggttgccgtt cgggtattcg
    36181 gtgtaccaga tacccttgcc gccggccgcc ttgatggcct ccaccagttc ccgggatttc
    36241 tcaaccggga cggacttgtc ggccgcgccg tgaaagcacc agatcgggat gtccctcaga
    36301 cgcggggcaa ttttctcggc caaccccgtg gactcgccga attcggcaaa accgcagatc
    36361 ggcacgaccg ccgcccacct atcggggtaa cttgcggcca gcatccaagc cccctgtccg
    36421 cccagtgaga ggccggtcag gtatacgcgc ttcgggtcca ggttgtactc gcgcgaggtc
    36481 agctcgacgc agcgcaaagc catgcccgcc attgggccta cccaggactc attcggccgg
    36541 cattgtggca tgacgactat ggccggaatg tttcgatggt gccggcggat cgcgcggccg
    36601 atgccaatat cggtttgtag gaatccatcg tcgccgcgct cgccggcgcc gtggaggaag
    36661 aggatgaccg gccaaggccg ctcgggagtg tagtccggcg gcacgtagac gacgtaggcg
    36721 tagctctggt catccacgct cacagtcttg cggatgaagc cggtcaccgt cacggaggac
    36781 ggagcggcag cggccgtctg tgtttcgggt gcaacgccgg ggccggacgg aagcggctgt
    36841 gcgaacgcga tcaagaacat cgtggcgatc cagacgagat tcagcataaa cggtatctct
    36901 ggctaccggc gttcaggccg ggagggctca tgctgtagcg gcgcggcggc gcttatgatg
    36961 gaccgtaagg caggctgagt cgacgcccaa ggcccgcgcc tgtttaaaaa caagcggttt
    37021 gaagacattc cggcgcgaca cgcgcgagca gtttgttcgg ataaaccagc aggtgcggcg
    37081 gtgcccaatt accgggcaat tcgacaaaaa tcgccaaact ggtcgcctcc tgacccgcag
    37141 ccaaggagtt tgcggtcagt tcgagttgaa acccgcgggc cccatgcggc ccttcacgct
    37201 tcttcagccg ccgtcgtcgc gggcatcgtt tgcggcgccg gaacatgttc ttgcggccgg
    37261 cgcgcttccg gcgggcgaac gacggccggg cgctcgggcg agctttgctc gtcctcgtcc
    37321 gccccgcctt caccccaggc gcccttcacc ggcgagagga tcatgatcat gttccggccg
    37381 tccatcgagg gcggccgttc caccttcacc ttttcgccga gttgatccgc gatttcgcgc
    37441 aaggatgcga aagcgatctc gcgatgggct cgttcgcggc cccggaatcg catcgtgaac
    37501 tgcaccttgt cgccggcctt caagaacttt tcggcctgcc gcaacttgat accacgatcg
    37561 tgatcgtcgg tcttgggccg caggcgaact tccttcagcg cctgctcgtg gctctttttg
    37621 gtcttcttct tctgcaagta cttccatttg ccgtagtcca ttatgcggca aacgggcggc
    37681 cgcacgttgg gtgcgacctc gaccaggtcc atgcccgcct cgtgggccat ccgcatggcc
    37741 tcatttacgg gcatgacccc gagctgttcg ccggcttcac cgatgacgcg caccggcgag
    37801 atgcgaatct gctcgtttct gcgcatttcc tgagcgatgt tacttaacct ccttcagcgt
    37861 gcgtcagtga cgcaccttct accgccgggg cctggccgcc cggcgccggc gtcttttcgc
    37921 cgtgagtggc aatctcagtc gcgcacgcgg ccaggaagtc ggacagccgg aaactgccgt
    37981 actgctgccc ttcgcgcgtg cgcacgttta ctgctccgtt ccgcatctcc tgttcaccga
    38041 ccacgaggat gtacggcacc ttcatcatgg tggcgctgcg gatcttagcc ccgattcgtt
    38101 cgctgctgtt atccagctcc gttcgcagtc cggccgtgcg gcaggccgct gcaacttgcg
    38161 ccgcatacgc ggcggacttc tcgctgaccg aacagaccgt cacctgaacc ggcgccagcc
    38221 acagcgggaa tttacccgcg aaatgttcga tcagaatgcc gacgaagcgc tccatgctgc
    38281 caaacagcgt gcggtggacc atgaccgggc ggtgctcgga attgtcctca ccaatgtacg
    38341 tcaggccgaa acgctcgggc agattgtagt ccacctgcac cgtgccaagc tgccattcgc
    38401 gaccgatgca gtcgcgcacc acgaagtcga tctttggacc gtaaaaggcc gcttcgccca
    38461 cttcctcgga cgagttcaag gcggcggccg caaccgagcg gcgcagggct tcctgcgcca
    38521 tgcgccagtt ctcctcggaa ccgatatatt ttttgctgtc cgggtcgcgc agcccgatcc
    38581 gcacgcgata gtcgttcaac ccgatcatct tcagcacgaa ctgcgacaga tcgacgcatt
    38641 cggcgatctc atcgggcaac tgctgcggcg tgcagaatac gtgggcgtcg tcctggcaga
    38701 agccgcggac ccgcgtcatg ccgttcactt cgcccgattt ctcatagcgg taaacctggc
    38761 cgaactcggc cagcttgacc ggcaactggc ggtagctccg cgcctccgac gtgtaaatct
    38821 gaatgtggtg cgggcagttc atcggcttga gcaagtaccc gtcttcaacc gccagccacg
    38881 cccggatgcg ccgctgacgc tcttcacgcg gcgcgctctt cggataaccc gcctgccgca
    38941 aatcctcgtg ctcggccgca aggtcgttga acagctcgat ttccgccggg ctggcctgct
    39001 cacccgcctc tccggccagt tcccagagcc ggttcagcat ccgggcccgc tccgactcga
    39061 acagcggcgg gtactggctt tcccggtagt acgggaagtg cccgctggtg cgatagaggt
    39121 cgaggcgccc gatgtgcggc gtgtaaacca attgataacc gcgggcgatg agttcttttc
    39181 gcagctcctc ttccagcacg tgccggatgg tcgcgccgtg cggcttccac agaatcaacc
    39241 cggtccccac cagtgggctg atcgtgaaca ggccgagttc ctgccccagc ttgcggtggt
    39301 cgcgcttctt ggcttcctcc atcatgcgga ggtattcctg aagcgccttc ttgttcggcc
    39361 aggtcgtgcc gtagacgcgc tgcagcatct gcttggtggc gtcgccgtgg tggtaggccc
    39421 cggcaacctg catcaccttg aacgcgccga tgcggccggt actgggcacg tgcggcccgc
    39481 ggcagaggtc ctcaaagctc ttgcccggct cgccggtgga gtagaagctc agcgtgtcgc
    39541 cctcggcccg caacgcgttg tcgatcttgt attcgttgcg ttcggcttcg agcttgcgca
    39601 tggcctcgtc gcgcggcatc tcgtagcgga cgaagggccg gtcctccttc acgatttcgg
    39661 ccatcctggc ttcgatccgc tcgaaatcct cgggcgtcag gctgtagtcg aggtcaatgt
    39721 cgtagtagaa accgttgtcc acgggcgggc catacaccag cttcgtgttc ggccaaagct
    39781 gacaaatcgc ttcggccatc acgtgagcgg cgctgtggcg ggccacgtgg agcccctcgg
    39841 gatcgtcgat tttcagagcc gtgagcttta cttgcccgtc agtcaggcgc gtagagagtt
    39901 cgaccacctg gccgtcgact ttggcggcga cggcctgatc ggccaagcgc gggttgaggg
    39961 cgcgcagcac atctacgacg cggaccccgt cgggatgctc gacaacagtg ccgtcgggca
    40021 actgcacctt caccatgtgg acaaattcct catgggagcg ggttcgctac agtcgccgac
    40081 gccagcagga attgcatacg ggctaagttc gacacttccg ggacgaaacc atgccgggtc
    40141 cgcggctgtt cgccgctcca agttaggccc ggtcatgtct gcgtgtttcg caaaattcgt
    40201 cccatgtgtt ctaaatatcg gccgcccaac ggcggcacta tacaacggtt ggagtttatc
    40261 gggcaggccg cgcaaaagtc aaccccaaat cggccccttg cagcatcgcc tggacgcccg
    40321 gtgtctgccc gtctctgccc tgcctccccc cttgcggccg gcccggttac gaatcaaact
    40381 gcgcgggtgc gtccgaagaa ccccgtgaat taaagaaacc aatgaattcg gcgttacaga
    40441 tcgcaaccga ggtctcagcc gggcggctga aagcggagca ggtttgccag gaagcactgg
    40501 cgctcatcgc gcagcgtgac ccgtccctgc acgcatttct ggagctgacg ccggagcacg
    40561 cccggcagcg cgccgccgcg atcgatgcag ccgtctcggc cggtcagccg gtcggccccc
    40621 tggcaggcgt gccgatcgct gtaaaggaca atatctgcac cttggcgggc cggacccggt
    40681 gcgcttcgaa gatcttggag gaataccgct cgccgtacaa cgccaccgta atcgaccgcc
    40741 ttgaggcggc cggcgccgtg atcgtcggaa agaccaatat ggacgaattc gcgatgggct
    40801 cctccaccga aaatagcggc atgggcccga ccggaaatcc ctggaacgcg gatttcgtcc
    40861 ccggcggctc gtccggcggt tcggccgtcg ccgtcgccgc ccgaatggtc cccctggcgc
    40921 tcggctccga caccgggggc tccatccgcc agcccgccag cttgtgcggc gtcgtgggcc
    40981 ttaagccaac gtacgggcgc gtatcccgct acggcctggt ggcgtacggg tccagcctgg
    41041 accagatcgg gccgctggcc acgaatgtca cggatgccgc gctgctgctg gaagtggccg
    41101 ccgggcagga tccgcatgat tcgactagcg tttccgagcc ggtgccgcag tacacggccg
    41161 ccctggagga ttcgcagctt gcccaatgtg cggccggcct tcgcatcggc gtgccgagag
    41221 aatacttcgg ggaagggctg gatccggaag tgcggacggc gattgaagcg gcgctcgagg
    41281 tttatcgcgg gctgggggcg aaaatcgtcg aggtctcgct gccacactcg ccctacagca
    41341 tcgccggtta ctacgtcgtg gcgacggccg aatgctcgag caacctggcg cggtttgacg
    41401 gcgtgcacta cggccgccgg acggccgcgc cgaaggacct catcgatctg tacagctcct
    41461 cgcgtgatga gggcttcggc ccggaagtca aacgccgcat catgctgggc acgttcgtgc
    41521 tttccagcgg ctactacgag gcgtattaca gcaaggcttt gaaggtgcgg cggctgatca
    41581 aacaggattt cgaccgtgcg ttcgagcagg ccgacgtgct ggccggcccg acttcgccga
    41641 cgcctgcctt ccgcctgggg gagaaggtca gcgatccgct ggcgatgtac ctggcggatg
    41701 tgtataccat ctcggtcaat ctgggtggtc tgccggcgct ttcgattccc tgcggtttca
    41761 gcgccgccgc cggtctgccg atcggactgc agttgatcgg cccgctgttc ggagaagccc
    41821 ggctgctgca agcggcccgg ctctacgagc gcgagaccga ctggcacacg cgcgagccgc
    41881 cactggtggc gcaatgtccg aactagaacc cgagccgcgg ctgacgcacg aaccggaacc
    41941 cgcacaggaa ggtcccgaag cgcgggtgcc atgcccaagc tctaagcgcg ggcatgcccc
    42001 ggccaccccc cccgacgctt gcgaccaggc acacgaagat tacgaagcgt gccaagaact
    42061 cccgacgcct atttcagagg gggcatcgcc gcagtcagac gccggcgcgc caggattcga
    42121 ggacgccgcc atctgcggca tcatcatact gctggccttc gtcctgcgct ttatctacat
    42181 cctgcaatgg caacgcaatc caggcttcgc gcacccgggc atggacgagc tctaccacga
    42241 ccagtgggcg caagccattg cagccggtaa ggcgttcgtg gaagggccat acttccgcgc
    42301 cccgctctat cccgctttcc tgggcttcat atacaagctc ttcgggcaca attacctggc
    42361 accgcgcatc gtgcaggccg tgctggactc actcagttgc ggactgctct tcatcgccgg
    42421 ccggcaggtg ttcagccggc gggtagccgc ggtcgccggt tttgcggccg cgacttactg
    42481 gatgctgatt ttcttcaccg gcgagttact gatcgcgccg ctgctcgtat tcctcgatct
    42541 gctgctgata attctgctgc tgcgggccgg caagaagccc ggagcgctgc tctatggact
    42601 ggcgggcttg gtaatgggcc tctcggcaat cgcgcggccg aacatcctgc tcttcgcgcc
    42661 gacggttgtc ctttggatgc tcgtgcgaca ttggcgcgag ctgcggcgtg gcctggtcta
    42721 cgtggcctgc atcacggtcg gttgtctgct gccgatcctg ccggtcacga ttcgcaatta
    42781 cgtcgtcgga aacgatcgcg tgctgatcgc ctcacagggc ggcgtgaact tctatatcgg
    42841 gaacaaccct tggtcagacg gacgaaccgc catcgtaccc ggcacacccg gcggctggtg
    42901 ggaaggctac tatgcctcga tcgcgctggc cgaggaggca gaggggcgca agctcaagcc
    42961 ctcggaagtg tcgagctatt attaccgcaa gggctgggag ttcatcacac agcagccggc
    43021 caaggcgatt tccctgtcgg cgctgaagct cagtttgttc tggagcagtt gggaaattcc
    43081 caatgacaag ggcgtctact tctggaccga gcgctttacg ccgattgtca aactgctgcc
    43141 gctcggcttc ggggtggtcg gtcccctggg aattctcggg ctgttcctga gccggcggcg
    43201 cgggcgtgag ctattcccgt tgtggggctt tatcctggtc tattcggcca gcatcgtggt
    43261 gttcttctgc aacacgcgct atcgcatgcc ggtcattccg cccctgatcc tcctggggac
    43321 ctgggccatc tttgaaaccg tgcgggccgt gaagcagcag cgctggcgga cggtcggcgc
    43381 cagcgcggcc gtcctggctg tcgccggcat cttcgtgaac gtgaccccgg gttcggaaaa
    43441 tttccgcacc gatgccgacg actggttctt cctggcaatg gagtacgagt cccagggcca
    43501 cggcacgccc gcactccgtt gttacgaaca atctttgcag gtccgcccgg acttcctgat
    43561 ggcccggctg cgctatggca aactgctcat gagggcgcaa cggccgaccg aggcgatcgc
    43621 gcagtttcgg caggcgctgg cggcgccccc gcgactggcg actaatgaga gtcaggctac
    43681 tctggccgaa gcgcacgtcc tgctgggcgc cgccctggcc gaaaccggcg ctttcgccga
    43741 ggccgccgcc gaattccagg ccgccattcc cagcctcccg ccggacaagt caattcgcgc
    43801 gcaggtcagc ttgggcagag ccctggccgc gctcggccgc aatgcagaag cgatcgacgt
    43861 tttcaagcaa gtgctgcaag tcgatcccgg caacgaccgt gccaccactg agctgcacaa
    43921 gctcggcctc acgccgtagc aagtcctcca taattaggga cagtcacgtg actgtccctg
    43981 attacgtgaa ttcaacacaa agaaaatgtt gattccgaac acctgaatct ttatagtgca
    44041 attaattcgg ccgctctgag gccgaggttc atttcccaca aggagaagga gcgacattcg
    44101 atgaacgcgg aacaccttag ccaaacatgg cgcaccactc tcgcattcgc ggcgctgctg
    44161 gtcgtcgcac accccgctac ggccgtcacg atcgaagact ggagcagcgg aaccgacgcc
    44221 gcctggacgc gcgtggacct gttggaactc tacggcctgg gcggaaccag ttacaccgtt
    44281 tccggcgggc aataccacat gacttcgaac tacccgctgc caccggtgcc ctacctgttg
    44341 ggcagcgcgt ccgtgttcag tgaatcgttg ctcgacccgg acgcctacgg caacggctat
    44401 ctcacgacgc gctttacgat tcacaacgcc aattcgtgcg tgtttgtacc catgcgggcc
    44461 gacgtagccc aaatggactt catcagcttt ttcgccgaga acgcctcgga cagcatcggc
    44521 atgaccgtct tcgacaactt cgagtacgtc aacggcgcca gcgccagctt caccattgag
    44581 gaagaggtcg agtattacct caccgccggc gcggtcggga ccgagctctc gatcaaagtc
    44641 tggccggtcg gcagccccga gcccgcttca ccccaggtga cgctgatcga cacgacgtac
    44701 accagcggcc agatcggcct gggcgcctac aacagggaag gcatgggcgg cacaatggcc
    44761 gccagcttcg gcgaggtaag cttcatcccc gagccggccg gcttgctgac actgctcgcc
    44821 gccctcggac tgctggccgc gcgccggcgg aaataaagca gcacacaacc gccccggcgc
    44881 tcgcggcaag agcagcgccg gggcgtgtcg tccccgccgg agcagagccg gggcgtgccg
    44941 cccagaatcc ttcaccggcg cagtacccgc tccgactacg ctgtgccgcc cccgaacggt
    45001 gccgaggact ctctctgtac ccccgccctg aatgcagtcg aagcgaatgg tcatcggccc
    45061 ggtcgcacgc caccgtttac ccctccgtcg gaacacctga ccgctacagt tgacgacaat
    45121 acgggtcgga ctactcgcaa cggccgtcag cagccagaac actgctttgg gcggacccaa
    45181 ttatcccgtg ctcgctgcac tcgctcagcg ggcgttcagt gacatataga gccggtgcaa
    45241 gaggtgtttg acacgccggc tgggctgagt gcgccggctg gccagactca gaccagtaac
    45301 cggcataaac tcaagctgcc tcgaaccaag ggagtcaaac agcccggaac caaacggagt
    45361 atctagcatg gcagaccagc agcagctatt tccggccgcg atgaaagaac tcgccgaaag
    45421 gcgaaagagg ctcgcaccgc aggcgctggg agctttcaag gctttcagcc aggccgtctt
    45481 cgccgacggc gccctgccgg gcaagacgaa acaactgatc gcgctggccg tcgcgcatgt
    45541 cactcaatgt ccctactgca tccgtgcgca tacgaagcag tcgctccagc atggcgccac
    45601 agccgaagag atcatggaag ccatctgggt ggcggccgaa atgcgcgccg gcgcggccta
    45661 cgcccattc
//
LOCUS       DAOSYS010000003        42565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_742_length_42565_cov_5.421266, whole genome shotgun sequence.
ACCESSION   DAOSYS010000003 DAOSYS010000000
VERSION     DAOSYS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 42565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42565
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_742_length_42565_cov_5.421266"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PLU99_00370"
     CDS             <1..1286
                     /locus_tag="PLU99_00370"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOO15535.1"
                     /translation="EPVDLLVIAAPAAAMPKVVDEVVASGKVGAAVMIPGGMGETEGS
                     GDLQRLVEEAVARGRELPDGGPVFLGPNCMGIQSRPGRYDTFFIPPKKLDTRWSAPAR
                     RVALISQSGAFIISRLSNIGTLDPALALSLGNQIDVTLADLLEAVGSRDDIDAIGVYA
                     EGFANLGGLEFVRAVAKARRAGKTIVFYKAGRTASGRSAAAGHTASLAGDYEVCTAAV
                     EQAGAIVTDTFAEFQQVLEVATALHDKQVGGRRVGAISNAGYETVGMADNITGMRYQV
                     EMATLSAETQKKLSETLAKFKLDKLVNARNPLDVTPMATDEAYEHCVRAMLEAPEVDG
                     VVASLVPLTAMMLTAADEITKPGSLAERLPRLFAGASKPVVVSIDSGELFDPFVKAIR
                     AAGVPVFRSCDQALRSLARYLCYRAPAASVGQNQE"
     gene            1482..2321
                     /locus_tag="PLU99_00375"
     CDS             1482..2321
                     /locus_tag="PLU99_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15536.1"
                     /translation="MSDPPVVPAGAIPDGLIPHTFYTFQVVYLPDLTQKYGLEIHNGP
                     GEFRATMNLVNGWMHTGAGPIYMRDSTTAANTVATGMAVGNVIDALAGLGFAGAGPIA
                     PTSSAAALANIVPGLAEVRPGTQLQGSGGTPEMHRIPGYAELYIYEPTLVEMWINGCK
                     TKRVEWALVGGGPIQMDRDWLAISGDVPPQTAENAPNSEGRLEQLRRQIDGELPGLLK
                     AQNFMLADGRPAEKTVRVEREPGTQIMKIRLNGKLSENTQEQLKQVLRERFGPDLGFA
                     FLD"
     gene            2326..3303
                     /locus_tag="PLU99_00380"
     CDS             2326..3303
                     /locus_tag="PLU99_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15537.1"
                     /translation="MASRRNATALLQHYGPRLLRFPNVVAVAIGKKQTKGRTRKTGWC
                     IRVHVTTKTSRPKKRVPPVLGPPRGRAHLGSIETDVIETGRAVLQGQVRPGFPMRIKV
                     RAVVGCLLRDHLQQDYILTAGHVVDFRLVVDERRNGNLKFGAQPHAYVTTPDGVESLA
                     GPCVRCSGRPPLDLALVLLTVGRAVPIAQIFGVRDLRQNPLRPVESIRVLRARGAFTT
                     WFHEGADADGMVTFEYPRPTGTGTIAVTYREIVFYPRSGDGGTLTRGDSGSAVVDSDN
                     RLLGIHIGASTRRQGADTHLLGYALPTHVAARWASGFSLLTPAGPVAWQ"
     gene            3488..3958
                     /locus_tag="PLU99_00385"
     CDS             3488..3958
                     /locus_tag="PLU99_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOO15538.1"
                     /translation="MRLLSPAFAERDPIPKKYTQDGANISPPLVAVDLPRGVREYALV
                     MDDLGSPQRQPRVHWVIYKIPPEVHELPENIARREVPPRPVGARQGLNSWRHNVGYRG
                     PAPPSGDGVHHYMFTLFALDAPVQLASGVSKGALLNAIRGHVLAQASLIGTYKR"
     gene            4087..4410
                     /locus_tag="PLU99_00390"
     CDS             4087..4410
                     /locus_tag="PLU99_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOO15539.1"
                     /translation="MQTQIVIRNAEQSNGLRAFAEEKFAEALARFEQNVMTAVMRLED
                     ITGPDKETGRDKLCSLHVKLRTGDIVIKQEHTDFRAAIDVALDRLKVQLGREIGRAKR
                     GIAEG"
     gene            4477..5337
                     /locus_tag="PLU99_00395"
     CDS             4477..5337
                     /locus_tag="PLU99_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO15540.1"
                     /translation="MSGPADGPLLSVKDLRTYLPARHGAGRGKSSRDRNGSGDAADVI
                     RAVDGVSFTLDRGQTLGLVGESGCGKTTLARTILRLIPATSGQVHFDGRDVLALRGAA
                     LRAMRRDMQIIFQDPLGSLNPRLRVESIVGEALTVHGLVRSAAERRERVAELLRRVGL
                     SPDALWRYPHEFSGGQRQRIGIARALALRPKLIICDEPVSALDVSIQSQILNLLNELQ
                     AEFGLSYLLIAHNLAVVRHASDRVAVMYKGRIVELAPTDELFDNPQHPYTRTLLAAVP
                     REAWGSAVQM"
     gene            complement(5369..8041)
                     /locus_tag="PLU99_00400"
     CDS             complement(5369..8041)
                     /locus_tag="PLU99_00400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO15541.1"
                     /translation="MNSKGKNSDASAAQRLRYAAFPRGSRRERLARWLLHNQRRFVFH
                     AQQGPSALTRALGRKLRLAGRRLLYRHKPLASVALTTGPGTDPAFPGQAPGLVSIVLP
                     VYNQAGLLRESIDSVLKQTYTNWELIVVDDGSADDVTAALEPYLREPRIRLLTQPNQK
                     LPKALSNGFDFARGEFWTWTSADNLMEPTQLEKLVGFLRAHPEAAMVYSDYLAIDDRG
                     QPLRDPAFRPQNRRSPDAPEIHLPRNTANLNVVQDNFIGASFLYRGWVGRLLGDYLPW
                     QGVEDYDYWMRMNSLFPVCHLGTDELLYRYRVHDNTLNARAAEHKIHDHAARLMTYEA
                     GRTGYYRQGWTILVDEATAAWLSTLTPARCKLQRLGSEPPADYGGKTLILVRADALPS
                     LSNHPRSPDLCIAAWFAGPEPNPYDVAVEIQSLADVCFADDKTTLARLGLLTRAAFRA
                     APGPALVDLALAYANNHLFTNKTISKADARRELPRAYLPTGRRTRVLLQVEDFLQGGL
                     EHVVLDVAATLDPQRFEPALLVLGRAGPAAAKARQRGLRVLRLDAESAARESAYRDLL
                     RQEGIDLVNAHYSLFGASTAADLGIPFMQTIHNTYVWLSPQQIAEYRAADAHTTGYTC
                     VSNAVAAYSDLRLGLPPGKMAVVTNGVDTQALDAARSGLDRAAERTALGFAPEEFVFL
                     CTASLGEVKAQLFLVRALAAALRKNPRLRLALLGQAQSSPNETDYAQLVRREIDRLGV
                     GPAVTFLGYHDRPQKFYYLADAFVLPSFIEGWSLALAEALYAGLPIIATDAGAARELL
                     TRTGGRLLPVPYGSLGTIDEYRFYNSVRAANTQFVDALSAALLATAANPEPPTLPEIL
                     RQSFAREQAYRAYARIYEWVTTGGDFAAVRAWTR"
     gene            8205..8693
                     /locus_tag="PLU99_00405"
     CDS             8205..8693
                     /locus_tag="PLU99_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOO15542.1"
                     /translation="MTAKDVIKNTLDFCRMVTVEYLKDFADADLLVRPVPAANHTAWQ
                     LGHLITSEHEMISALGHKMPELPAGFVERYTRESARSDQPAEFATKQEYLDLLEKVRA
                     GTLAALNATPEADLEKPAPESMRAYAPTVSAVFLITGTHELMHAGQFVTVRRKLGKPV
                     LF"
     gene            8912..9760
                     /locus_tag="PLU99_00410"
     CDS             8912..9760
                     /locus_tag="PLU99_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO15543.1"
                     /translation="MRPDGTVNRAALPTVFNPDDLHALEMALAVREAHGGTVTVLTMG
                     PPKAGEVLREAVYRGADRAILLTDRRAAASDTLATSYILSMAVRKIGRYDFVFCGRQA
                     IDGDTAQVGPQTAEKLGIPQVTYLEQVVDLHENIARLRRNVGSGWEIVEVRLPVLVTV
                     LDTAGLVRPPAAKRVLKYKRARTPSELLIEVSRENPDFSDQQKEAEALRRAESLRCRG
                     LMLEEWNLDDIGADLAWCGLSGSPTKVHRVQSIVLTKQGYTEIPPTDDGIRGLIRELV
                     IDHTLG"
     gene            9870..10904
                     /locus_tag="PLU99_00415"
     CDS             9870..10904
                     /locus_tag="PLU99_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020503135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO15544.1"
                     /translation="MIPVNRSGEVWVFAEQEEGSLHDIALELCGKARQLADQLGVKMA
                     AVLSGWNVRELSQRLFAHGADRVYLVEDRRLEHYQTQPYAKILCTLIDKHRPQIVIYG
                     ATPLGRDLAPRIASAMKAGLTADCTALEISDVTDPKTQQVHKNLLLQIRPAFGGNIIA
                     TIVNWDRWPQMATVREGVMPMLEPDMQRTGEILEEQVALTDADFPLRLVERHREPRKV
                     NLKGARTIVAGGGGVGSKANFRIIHELAGAIGGAVGASRAAVDGGYIGKEHQVGQTGT
                     TVRPALYIACGISGAVQHRAGMEESAKIVAINWDKEAPIFGVAHYGIVGDLNEVIPKM
                     IKALKERPKE"
     gene            11015..11854
                     /locus_tag="PLU99_00420"
     CDS             11015..11854
                     /locus_tag="PLU99_00420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOO15545.1"
                     /translation="MTLFAGDCRELLARLPARSVQLVVTSPPYNIGKTYERRVSLPDY
                     LQREREVINLCADKLRAGGSICWQIGNHVAEGEIFPLDTLLYPYFKERGLHLRNRIIW
                     HFEHGLHCSRRFSGRYETILWFTKDDAAGQYYFDLDPVRVPQKYPGKRHFKGPHKGEY
                     SANPLGKNPGDVWIIPNVKHNHPEKTVHPCQFPVELVERLVLALTRRGDLVLDPYMGV
                     GTTPAAAVVHGRRAAGAEIVPEYVKIARERVAQAVSGTLPARPRSRPVYVPSPNSPLV
                     KRS"
     gene            complement(11957..13198)
                     /gene="kbl"
                     /locus_tag="PLU99_00425"
     CDS             complement(11957..13198)
                     /gene="kbl"
                     /locus_tag="PLU99_00425"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOO15546.1"
                     /translation="MFGKMKQDLQKELVSIREAGLFKEERYILTPQAADVQVEYPPGA
                     PKKEVINFCANNYLGLSSHPRVIEAAHEGLKTHGYGMSSVRFICGTQDIHKELERKIA
                     DFFHTEDAILYSSCFDANGGVFEPLFGKDDAIITDALNHASIIDGIRLCKATRHIYKN
                     NDMADLEAKLKETQNCRYRVIATDGAFSMDGTIAQLDKIVALAEKYDALVMVDDAHCT
                     GVIGKTGRGSTEYCGVMGKVDIVTGTLGKALGGASGGFTTGRKEIIEMLRQRSRPYLF
                     SNTVSSIVVKASIAVFDMITESTELRDKVEANTKRFRKGMKEAGFDIKPGDHPIVPIM
                     LYNAKLSQDIARDMYSEGIFVIGFFYPVVGKGLARIRCQMSAAHDFSHIDRTIEAFTK
                     VGKKYDILHKTKEEIIAKYGE"
     gene            complement(13399..14514)
                     /locus_tag="PLU99_00430"
     CDS             complement(13399..14514)
                     /locus_tag="PLU99_00430"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO15547.1"
                     /translation="MATMKAVVKTKPEFGAVMQDKPVPQPKSDWVIVKVRATSICGTD
                     VHIYKWDPWSAGRIGAAKLPQILGHEVAGEVVEVGEHCKRIKVGDYISAETHIFDPGD
                     LTTMLGSPHVGQHMKILGVDFDGCFAEYFALPESICWPNDPSIPPEVATIQEPLGNAT
                     YAVLGEDADVAGKSMLITGDGPISLFAIGVARSVGVAKIIHFGKYDVNMQIGKKMGAD
                     HQMYTNKTTAKERFQFVMDQTGGNGVDIALEMVGSQDSVDDCFACVRKAGRITAFGVA
                     AENRLPVDYNNGIVFKACQIHGINGRKIFDTWYRNRNLLANGRLDPTPVITDIFPLSE
                     FEAGFNKMLERPRTSAKVVLFPDRSEYEAAMKRRGQK"
     gene            15311..16708
                     /locus_tag="PLU99_00435"
     CDS             15311..16708
                     /locus_tag="PLU99_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO15548.1"
                     /translation="MAMSVTDNNTLTLLNTMDYLSAQQGAIFRRLCTGSKINRGADDP
                     AGLMALQSINCELIGVDAAIRNNQRSDAMLNVADSALTQLSDLLGQIQTLVAQSSNSA
                     GLTANEIASNQSQIDDAIASIERIVRTTEFNGRNLLDGSLGINVTGANATYFENVRVY
                     SRDPSAASNAVRVTVNGVAQRAKAEGLTTTAASQATTIEVKGRLGTQIIDLKADENLS
                     AVVAKINDVTALTGVYASGTSAGVDLYSKGYGSAEFVKVSILSGDTTHFKELDGAGVD
                     ADVTVNGAAASADGNEVYFSQNGLNLAFRITEAFTAGTTQTFEVDKTGGATFQLGTDA
                     TTRYTLGIDSLSPRRLGGKSAGGFLSDLKSGGAASLAKNPAAAAAIAADAQKQVATMQ
                     GRIGSFQKYQIEISKNTMAAIKESLTTVKSSIADVDYAQETAALARQDVLMQSVASML
                     SVASQQSAQRILSLL"
     gene            complement(16948..18147)
                     /locus_tag="PLU99_00440"
     CDS             complement(16948..18147)
                     /locus_tag="PLU99_00440"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOO15549.1"
                     /translation="MKYAIILPDGAADVPSPQLGGRTPLAAAPIPNMDAIARTGRLGR
                     VLTVPAGFIPGTDVASMSLFGYDPHVYYSGRAPIEAAARGLPIGPDEVIFRCNFVTIL
                     GGRMCDFTAGHISQDEADQLIAELQSLAASDPALAGCKFHAGVGYRNLMLASPVAAYD
                     VKCTPPHDIPNQPVFGHLPQGPGAERVLKIMERGAEVLADHPVNQARRAAGRDPVSDI
                     WLWGQGRPRRFEPFAERFGLRAAVITAVDIIRGLAISMGMELIDVPGATGYFDTDYAG
                     KGRAAVRALDDFDLIAVHVEATDEAGHAGNVTEKVKALERIDEHIVAPLLEKLRQFAE
                     WRILVVPDHYTPCTTTAHDATPTPFAMAGHGITPSNAAQFSEAAAAVTGVMVDTGHTL
                     MGQFLSH"
     gene            complement(18351..20936)
                     /locus_tag="PLU99_00445"
     CDS             complement(18351..20936)
                     /locus_tag="PLU99_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOO15550.1"
                     /translation="MAGGGQRVRDPVCGMEVDPASATNRCEYEGRTYYFCCRHCLEAF
                     RRDARRFTSEQPAPQPAPTGPGRIALPVQHGAPQAGQPPRREGEAAAEPRREAGGTAA
                     SQPKGEAPAEPHAEHRPAETGPYTCPMHPEVHQAKPGACPKCGMALERLTPAAAPAQV
                     QYTCPMHPQVISDRPGSCPICGMALEPLTVSVEEEEDPELRDMTRRFWISLVLTAPLV
                     VIAMGHAIPGLGHWLSPALRGWIELALATPVVLWCGWPLLVRGWQSVVHRSLNMFTLI
                     AMGVGVAWTYSLVAVVAPGIFPASFRDESGAVGVYFEAAAVITSLVLLGQVLELRARR
                     STSGAIRALLGMAAKTARRLRDDGAEEDIPLQEVHIGDRLRVRPGEKVPVDGTVLEGR
                     SAVDESMITGEPIPVEKQPGDKVIGSTVNATGSFIMRAERVGSDTLLARIVQMVGEAQ
                     RSRAPIQRLADKVAGWFVPAVILAAIVTFVVWSIWGPAPAMAFAILNAVAVLIIACPC
                     ALGLATPMSVMVGVGRGAQAGVLIRNAEALEVMERIDTLIVDKTGTLTEGKPKLVSLT
                     PAAGWTEADLLHFAASIERGSEHPLAAAIVSGATERGIEPAEAQEFESVTGKGVRGRV
                     NGRAMALGNAGFMEQLHVATSAMLARAEELRRDGQTVMFVAVDGKPAGLLGVADPIKG
                     STPEAVRMLRDAGVEIVMVTGDSRTTAAAVAGKLGLTRVEAEVLPDQKAAVVRRLQQE
                     GRKVAMAGDGVNDAPALAQADVGIAMGTGTDVAMESAGITLVKGDLRGIAKARLLSRA
                     AVRNIRQNLFFAFIYNAIGIPIAAGVLYPVFGLLLNPMIAAAAMSFSSVSVVGNALRL
                     RRVQL"
     gene            21726..21798
                     /locus_tag="PLU99_00450"
     tRNA            21726..21798
                     /locus_tag="PLU99_00450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21759..21761,aa:Thr,seq:tgt)
     gene            21945..22460
                     /gene="tpx"
                     /locus_tag="PLU99_00455"
     CDS             21945..22460
                     /gene="tpx"
                     /locus_tag="PLU99_00455"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOO15551.1"
                     /translation="MIERSGVTTFQGNPLTLIGPALNAGSAAPEFVLTANDLSDLRSD
                     AYRGKLLVLSVVPSLDTPVCATQTRTFNQRATGLGEDVVVLTVSMDLPFAQKRFCGAE
                     GIQRVVTASDYKHRSFGPAYGVLIKELGLLARAVFVVDRAGKVTYSEYVPEVTQEPNY
                     DAALEALKAAK"
     gene            complement(22703..23998)
                     /gene="aroA"
                     /locus_tag="PLU99_00460"
     CDS             complement(22703..23998)
                     /gene="aroA"
                     /locus_tag="PLU99_00460"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOO15552.1"
                     /translation="MRVTWSGADVHLHPTGPIRARVRPPGSKSLTNRYLLCAALADGQ
                     TILDGASLAADADRMVTCLRELGIVIGIRHTNPPQIAVAGCRGHLPAGEAELDAGDAG
                     TVMRFLTALITLGYGRYRLDGSPRMRQRPIGPLVGALQGLGAPLGYDAEPGFPPVTAM
                     ARGLVGGRVRLDRLASSQFVSALLMVAPYAMSDCFIALRGRPPSEPYIEMTVQLMRQM
                     GVEVLGDGGRFIVPAGQRYRGGEYAVEPDASAATYLWGAAAVTGGAVRVDGLSRASLQ
                     GDVHFVDLLEAMGCSISAGPDFLEVSGPAAGRLRGIDVDLNPMPDTAQTLAVISLFAD
                     GPTTIRNVPNLRLKETDRLGALVTELARLGARVEQRDDGLTIYPPPRVQPATIETYND
                     HRMAMSFALAGLAAEGITIRNARCVSKSFPQFFEVLQDL"
     gene            complement(24198..25808)
                     /locus_tag="PLU99_00465"
     CDS             complement(24198..25808)
                     /locus_tag="PLU99_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO15553.1"
                     /translation="MLRYLSRTAPPALFIGLSCLLFACEPQYIPEWLGGGWTRPKSEG
                     AGSYTGGGPAFTEELVQARTFTNGTAVTTVTYLLNDLDGADLIPDGDVLSRTPYGIDF
                     NGDGKIDPVVGYGNEQAVIQILLSDPDTLPGFVRYTSLTLDSKRDMRNLTDVAVGDID
                     RDGALDIVASAEGAVWYFHHPTGLPTTALADWGNPDPNDELRERIDASYTMLSDSEIQ
                     AIISQALGPGVNIDDYIVTVEQRYRNVQIGDVDNDGDNDVIASRSFVMTCTPRPDVPV
                     EPIQITDGNVMAFINPGFSTTGHNWTAISLGTHERQSRLDRDGATGLLLYDLDDDGDL
                     DVISAAGEDNNAQVAWFENPVENRPQAPGPGLLVDTPWKQWRIGSVRNAWSIDIADLT
                     GDGRPDVVATGSNQQQMLLFVQPNAGPARSYDWDTYVLVTFETFEPRDLKIVDIDLDG
                     ALEIVVGGSAGAVRYFESSGDPTQVWHPVVITNFPAGGDVGWLGYGDLDADGDYDLVT
                     VIETDQENDSRVSWIRNEVLALPGVASP"
     gene            complement(25932..27005)
                     /gene="waaC"
                     /locus_tag="PLU99_00470"
     CDS             complement(25932..27005)
                     /gene="waaC"
                     /locus_tag="PLU99_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HOO15554.1"
                     /translation="MLTPYQRILIIKPSSLGDIVHGLPVLAALRKTWPTAHIAWLVGD
                     SFAALLEGHPLIDEVIRFDRRRYGRLLRSPRALADFARFVFALRRRRFDLVIDLQGLF
                     RSGFLAWASGAPQRVGFAVARELAPLFYTRRVRCAPAAVHAVDKNLQIAAALGAAPAA
                     PEFPLHVPPHEIEQARNKLAAAAGRELREFVAILPAARWPSKLWNESHFAKLIERLAA
                     DGQPTCVLLGAPSEAGIAQRITAACKAEIVNLVGRTSLPQLVALLDLSRFVICLDSGP
                     MHIAAALNKPLVALFGPTNPHRTGPYSPRAHVVSRALPCAPCYRVTCPLGHHACLDGL
                     LPEQVLSHVQNLPVGHATLTATP"
     gene            complement(27173..30313)
                     /locus_tag="PLU99_00475"
     CDS             complement(27173..30313)
                     /locus_tag="PLU99_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO15555.1"
                     /translation="MNISALFIRRPVTTTLLMAAILIFGIMGYRLLPVSDLPNVDYPT
                     INVTANLPGASPQTMASSVATPLEREFSTIAGIESMSSNSTLGSTSITITFDLSRDID
                     AAAQDVQAAIARAQRRLPPEMPNPPSYRKVNPADQPILFLAVTAPGLPLYELNEYAET
                     LMAQRISMVNGVAQVNVFGSQNYAVRIQLDPRELAARELGIDEVADAISRGNVKLPVG
                     TLYGSETAVTLESTGQLMDAAAYRPLIVAYRNGNPIRLEMLGRVIDSVEDDKRAAWFN
                     GVRGMVLAVQRQPGTNTVQVVDDVKALLPSFRQQLPAGVTISELYDRSISIRESVEDV
                     KFTLWLTLCLVVMVIFLFLRNLRATIIPSVAMPLSIVGAFAVMYLLEFNLDNLSLMAL
                     TLSVGFVVDDAIVMLENIVRHIEQGEGVLEAAYNGAREIGFTIVSMTVSLVAVFIPVL
                     FMGGLVGRLLHEFAITISAAILVSGVVSLTLTPMMCSRLLRPHAKQGHGRLYALSERF
                     FDGMLAAYEWGLRGVLRHRLATMLLSVAVLIPTAYLFVKIPKGFLPSEDAGRIMVSTE
                     AAEGISFESMVRHQLALVDIVKTDENVASFMASVGGGYMGGANAGRMFLSLKPRHERK
                     LSADEIIEQLRPKLAQVPGIRAYLQNVPPIQVGGRMSKSQYQFTLQSPDMEEMYAGAM
                     KLAQRISELDGFVDVTTDVQLKNPQVIVEIDRDKASTLGITAEQIELALSSAYAARQI
                     STIYAPQDQYQVIIELADEYQRDPAALELLYLRTRHGKLVPLQAVAKLVPAVGPLSVN
                     HSGQLPSVTISFNLKPGFALGSATAQVDALARELLPAGISTSFQGTAQVFQSSLKNLG
                     LLLIVAVLVIYIVLGILYESFIHPITILSALPFAGFGALLTLMVFDVELSLYAFVGII
                     MLIGLVKKNGIMMIDFAIEAQRAGHTPLEAIYQASVIRFRPIMMTTMAALMGALPIAL
                     GVGAGAEARRPLGLAVLGGLLFSQMLTLFATPVFYTYMEALRTWPERRRRRRSPDEHP
                     APARHATPALSSDARD"
     gene            complement(30310..31506)
                     /locus_tag="PLU99_00480"
     CDS             complement(30310..31506)
                     /locus_tag="PLU99_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO15556.1"
                     /translation="MEEFLISRQCLGARAAGPIPLLLATLIGLSGCDRLQPPAGAAGP
                     ARQPPAPVTIAAAVEKDMPLELHAIGRVQPFTTVTVKPQVSGQLAQTHFVHGQDVKAG
                     DLLFTIDSRPFQTALEQAQATLEKNIALMNDALIEAEWQENLQERGLGTQRESQNKRA
                     LAESLKAAVAADRAALAKARLDLEYCSIRAPGDGRAGEILAHPGNVVSANETALVVLN
                     QLSPIYVSFSLPERELGRIKRAQSTGDLQVRVEIPGEEQAENEVGVLSFIDNAVDSAT
                     GMISLKGTFGNANHWLWPGQFVNVTLTLGTRRGAVVVPSAAVQTSQTGQYVFVISEDL
                     VAESRPVVPGFAVGEETIIETGLRAGERVVTDGHLRVTHGTRVVVREPADVGQPAPAS
                     RPDTHP"
     gene            31773..32150
                     /locus_tag="PLU99_00485"
     CDS             31773..32150
                     /locus_tag="PLU99_00485"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO15557.1"
                     /translation="MASGFKPHTWGARPTASANLCYVAGQTEVARSRTPDRERRVPHR
                     APCRLQWWDSDSKRTASCVGETTNLSERGLAVRVRHFLLTGTRVEILLANLAGDPRRI
                     SGVVVRCQRILADTFELGIRFVD"
     gene            complement(32225..34597)
                     /locus_tag="PLU99_00490"
     CDS             complement(32225..34597)
                     /locus_tag="PLU99_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15558.1"
                     /translation="MVSARRARPSSSPDTAVPSESWKRVHTWALALIVLTACGLRLAF
                     WWGQSANNPFFSHPTLDEQTHHELAQAVATGQGVGAAPFFRAPLYYYLLGALYAIVGP
                     NPLAARLLGCLLGTFSCYLIARLALALAGPAVGLLAGLIAALYWPMIFFDNQLQTASL
                     EVSLDVLMMLLFVRGAQKGSWPLFLTAGLAWGLSAITRANILIIAPALLIALWLWPQS
                     QSRRTPRRWLWAALLLVGAALPVSAVTLRNRVVGGEWVGIASSGGINFYIGNNPRSDG
                     RSAIVPEIGYFWSGRFEGTQALPEAKAGRKLTHAEVSDFWFERATTWIRSNPADWVRL
                     MWRKWRLFFSPLELPNDHPIEYFAGLAPVARFFWIGFPLVGVLGIAGLTLLGKNWRTW
                     LLPLLFLPSYAAGIVLFFCPARFRLPVIPVLILLAAVGLVRGVQLLAARRFKPLAVYA
                     GLGALAAGFIGTNPPDRSTFEHEEESAAHLQLGQFYAKTAKEDAALREQALRHLQRAA
                     ELQPDDWSVRRSLGACFIELGLTEEGCEELSQAAQLSGNDPDNFYYLGTCLENVGRPE
                     EAADAYRQALELLGRTAGRPEYLADLHHRLGRILAARRDYAAAITQFEQALRHNPNDT
                     AALLSVGGALLELRHYDEAASRYRQLLAREPANVAALRSLAHALQLGGHAREALAVLA
                     EAWHHEPHNVSVAGPLAWQLATSPDVTLRDGPRALQIVQQAIEAAGPNPTLLQALAAA
                     YAECGRFDEAVTTAEQVLQMARQAGRVHEARWIEEQLALYRRNLPFRESE"
     gene            complement(34716..35828)
                     /locus_tag="PLU99_00495"
     CDS             complement(34716..35828)
                     /locus_tag="PLU99_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOO15559.1"
                     /translation="MDRPIRVLISKIGLDGHDRGAKLIVRNLRDAGMDVIYTGLWQSP
                     SSTVQAAIQEDVDVLGVSLLSAAHMTIMPEVRRLCGLAGVPDMPLIVGGIIPEEDGKA
                     LREQGIDGVFNPGTPMSAIIEFIRELAGRCRARRNAEKNPPDVSTPAGLARAMSWVAA
                     GRGAELNHLPAVDWPTVRIGITGSAGVGKSTLIGRLAGALLERKHRVGVIAIDPTSPI
                     TGGSLLGDRLRMMSAEPREGLFVRSLSSAGQTGGLGPHCEEISRLLTAAGYPIVLIET
                     VGAGQNDTEVRKLADEVLLLVMPGAGDAVQFAKAGVVEIATGFVVNKADLPGADTTIR
                     QLRDAIDNSRPIWGVSSLRGEGLDPLCDWIEARLAK"
     gene            36337..36840
                     /locus_tag="PLU99_00500"
     CDS             36337..36840
                     /locus_tag="PLU99_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15560.1"
                     /translation="MKRILAYNVPWVILLCACNSTSPRLNAPPHGCAEARHEMQAMYT
                     HMSDNALLADMNLSDVDFIPHRPMLSARGEERLCRIVALMQEYGGEIRYTAENGDRDL
                     VDRRIEVIIDFLTEAGLPAHAVREGLPGGRGMEAREVLLIKLVEGTYSPERSGGGEKS
                     GPGQYEK"
     gene            36854..37906
                     /locus_tag="PLU99_00505"
     CDS             36854..37906
                     /locus_tag="PLU99_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15561.1"
                     /translation="MLKYFVPPSLVLGAVLLWSTGCQSEPKSATPLRPAVEDSGPRLN
                     ASTYFAHGHLLERQGNYEGAVDQYQRALALAPQMTSARNRLGITLNKLGRHAEATAQF
                     RQAVQQNPTDGYLQNNLGFSLYLEGRYTEALAALAAALELQPDYGRAHMNKGIVLAKT
                     GDYAGALTEFTLAAGEAEAHYNLAVIQAEAGDYAAAARSLESALNVNPSFEAARQQLH
                     VVARLAAGAGTAVAAAPVAENALAEAPTVETPAAESPAAETPVAEAPATAVDTSAPPG
                     GAPAQDLAADGITPVRPEAASPELLAKMVEHWPLETAKQDGGELHTDRASARERQPVV
                     SAASAVLVRRPATVTQ"
     gene            complement(37910..39910)
                     /locus_tag="PLU99_00510"
     CDS             complement(37910..39910)
                     /locus_tag="PLU99_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HOO15562.1"
                     /translation="MQPSRFVPASVPTALEPAQPTPASAQFTPRAVLTGMLMGAVLSA
                     CNIYLGLQLGIGINMAIVAILLAYAFWSGLSWLTRGRVRPWGMLENNISQTACSAAAL
                     VGSAGLVAAVPALTILTGVYLPWHTLSLWILSVCLVGITIAVVLRRQLLLHDNLTFPM
                     GVACAEMLREMHTRGREALTRFAALLCGAAAAAGMSFAASFWKIAPFKLPGAVQGIKP
                     TVLTFMVQPSLLWFGVGGLIGFRAGASLLIGAVLAYGVMVPRLIKNGSIRLTVVEKVS
                     TLPAGVDLQKLAPEQARYEPAAGRLEWRGLMSAAQRDALIAASANADWREVIAKLYMR
                     SQPQNATLTATTFSDLVSWLVWPGATIMVVASLASFSLSVRTAWRSRSRNRQSSAAAP
                     TSGDVPWSWAAAGLIFVLAASVALQISLFGIAWWLAVGGVLLAFVLAIIATRVSGEAG
                     YTPVGQMGKVAQLTVGAMAPHNAVANLMASNVAAGAASQSADLMDDLKCGYLVGTPAR
                     LQTLAQICGVLAGSLAGSAVYLMFMPRENFGQGWNLPAVAPLKAVAELFQHGFSLIPP
                     GTGLAMIIAAGAGLILSVLEKGLPRRAARFVPSAASIGLAFMIPAGYSIALFVGGLTA
                     LMLSIAAPSGTKRFLVAICAGIVAGETLHKTGQALMSAFAGA"
     gene            complement(40235..40663)
                     /locus_tag="PLU99_00515"
     CDS             complement(40235..40663)
                     /locus_tag="PLU99_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOO15563.1"
                     /translation="MPKRQARACLPEKATVPGHAPHSTREYGDKGERLAETYLKRRGL
                     KLVARGYVTPVGEIDLIVRDGDTVVFVEVKTRRSRQFAEPQESVGAVKQRKMRRCAEW
                     FLRERNWTDRPCRFDVVGIVLPDEGEPEIEHFADAFQPKY"
     gene            complement(40761..41255)
                     /gene="rplS"
                     /locus_tag="PLU99_00520"
     CDS             complement(40761..41255)
                     /gene="rplS"
                     /locus_tag="PLU99_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOO15564.1"
                     /translation="MKHPLIDAVEKKYRRENELDFGIGETVRVTIKIVEGNKERLQDF
                     EGVVIARKGSGLDEMFTVRRLVGNEGVERTFPLHSPKVVRIKTVRRGKIRRCKLYYLR
                     ERVGRARKLRERRISAEARKAALEAQLAKARALREADEVAQAAREREAAAKTGSPEMA
                     TSEA"
     gene            complement(41277..42128)
                     /gene="trmD"
                     /locus_tag="PLU99_00525"
     CDS             complement(41277..42128)
                     /gene="trmD"
                     /locus_tag="PLU99_00525"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOO15565.1"
                     /translation="MRIDVLTLFPELMQPYLSASILGRAQQAGIVEIHTHQLRDYSTD
                     PHKKVDDRPFGGGPGMVLMCQPVIDAVYAIEKLDPRPALRLLLTPQGRVFNQAGAARY
                     AAAERLLLICGHYEGFDERIIELLQPEEVSLGDFVLTGGEVAALAVVDAVVRLLPGAL
                     GHEEAAGLESFQNNLLDYPQYTRPRVFGGLSVPEILLSGNHAQVDAWRRAQAEERTRQ
                     RRPDLLEGKCGTDFQDSPDKNGSGNDGSHCNGDRSIESTLALNCIDYCACPMAQAGPS
                     AIAKRSA"
     gene            complement(42174..42425)
                     /gene="rpsP"
                     /locus_tag="PLU99_00530"
     CDS             complement(42174..42425)
                     /gene="rpsP"
                     /locus_tag="PLU99_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOO15566.1"
                     /translation="MAVKLRLKRFGRSHRSVFRLAAMESRNQRDGRVLEELGHYDPAN
                     RNTDMQVRLDTDRIKHWLSQGAQPTETVANLLKRHGVTK"
BASE COUNT         7701 a        13449 c        13954 g         7461 t
ORIGIN      
        1 ccgagccggt ggacctgctg gtaatcgccg ctccggcggc cgcaatgccg aaggtggtgg
       61 acgaagtggt cgccagcggc aaggtcggcg cggcggtcat gattcctggc ggcatgggcg
      121 aaaccgaggg ttcgggtgat ttgcagcgtc tggtggaaga ggcagtggcg cgcgggcggg
      181 agttgcccga cggcgggccc gtatttctgg ggccgaactg catggggatt caatcgcggc
      241 cggggcgcta cgatacgttc ttcattccgc ccaagaagct cgatacgcgc tggagcgcac
      301 cggcgcggcg cgtggccctg atttcgcaga gcggggcgtt catcatttcg cgtttgagca
      361 acatcggcac gctggatccg gcgctggcgc tgtcgttggg caaccagatc gacgtcacgc
      421 tggcggacct gctggaggcg gtcggatcac gcgacgacat cgacgcgatc ggcgtctatg
      481 ccgagggttt cgcaaacctg ggtgggctcg aattcgtccg cgcggtcgcc aaagccaggc
      541 gcgcgggcaa gacaatcgtc ttctacaagg ccgggcggac tgcgtcgggg cggtcggcgg
      601 cggccggtca tacagcctcg ctggccggtg actacgaagt ctgcactgcg gcggtggaac
      661 aggccggcgc gatagtgacg gatacgttcg ccgagttcca gcaagtgctg gaggtcgcga
      721 ctgcgctgca cgacaagcag gtcggcgggc ggcgggtcgg cgcgatcagt aatgccggct
      781 acgaaaccgt cgggatggcc gacaacatca ccggcatgcg gtaccaggtt gagatggcca
      841 cactctccgc tgagacgcag aagaagctgt cggagacgtt ggcgaagttc aagctggata
      901 agctggtcaa tgcgcgcaac ccgctggatg tcaccccaat ggcgacagac gaagcgtacg
      961 agcactgtgt gcgtgccatg ctcgaagcgc ccgaagtaga cggcgtcgtc gcctctctgg
     1021 tgccgctgac ggcaatgatg cttaccgcgg ccgacgagat cacgaagccc ggctcactgg
     1081 ccgaacggct gccgaggctt ttcgccggag cgtccaagcc ggtagtagtc agcatcgatt
     1141 ccggcgagct gtttgatccg tttgtgaagg cgattcgggc ggcgggcgtg ccggtcttcc
     1201 gctcgtgtga tcaggcgctg cggtcgctgg cgcggtatct gtgttaccgg gcgccggcgg
     1261 cgagtgtggg gcagaatcag gagtagcaag accgcggctg ctgggtccgg gggacctgct
     1321 gcgcgtgcga tgaaccaccc agtgtacggg cgcgcccggg caagggtagt ggcacggcga
     1381 gacctcggca ctgcttgaag ctgcgcttca tgcagtggca tacaggactc cgtttgacag
     1441 ggcggttgca catcatttga cagctggggc aagtgctggg aatgtccgat cctccggtcg
     1501 ttccggccgg cgctatcccg gacggcttga ttccgcatac gttctacacc ttccaagtgg
     1561 tctacctacc ggacctgacg cagaagtatg ggctggagat acacaacggc ccgggcgagt
     1621 ttcgcgcgac tatgaacctc gtgaacggct ggatgcacac gggcgccggg ccgatctaca
     1681 tgcgcgatag caccactgcc gcgaacaccg tggcgaccgg catggccgtg ggcaacgtta
     1741 ttgatgcact ggccggcttg ggtttcgcag gcgcggggcc cattgctccc acctcgtccg
     1801 cggcggcgct ggccaacatc gtcccgggcc tcgcggaagt gcggcccggc acacaacttc
     1861 agggcagcgg cggcacgccc gagatgcacc gcattcccgg ctacgctgaa ctctacatct
     1921 acgagccaac gctggtcgag atgtggataa acggctgcaa gaccaaacgc gtcgaatggg
     1981 cattggtggg cggcggcccg attcaaatgg atcgcgactg gctggcgatt tccggtgacg
     2041 ttccgccgca gaccgcggaa aacgcgccga attccgaagg gcgcttggag caattgcggc
     2101 gacagattga tggggagctg ccgggccttc tgaaggccca gaactttatg ctcgccgacg
     2161 ggcggccggc cgagaagacg gtccgggtcg agcgagagcc cggcacccag atcatgaaga
     2221 tccgactcaa tggtaaacta tcggagaaca cgcaggaaca gcttaagcaa gtgctgcgcg
     2281 agcggttcgg cccggatctc ggctttgctt ttctagacta gccgcatggc atcccggcga
     2341 aatgcgacgg cgctgttgca gcactacggc ccgcgtctgt tgcgcttccc taacgtcgtg
     2401 gccgtcgcga ttggcaagaa gcagacgaag ggccgtaccc gcaagacagg ctggtgcatt
     2461 cgcgtccacg tcacgaccaa gacttctcgc ccgaaaaagc gtgtcccgcc agtcctcggc
     2521 cctccgcgcg gccgcgcgca cctgggatcg atcgaaaccg acgtcatcga gaccggccgc
     2581 gccgtcctgc agggccaggt acggccgggt tttccgatgc ggatcaaagt gcgcgccgtg
     2641 gtcggctgcc tgctgcgcga ccacctgcaa caggactaca ttctcacagc tggccacgtg
     2701 gtcgatttcc ggctcgtcgt tgacgaacgc cgcaatggca atctcaagtt cggcgcgcag
     2761 ccgcacgctt acgtcacgac gccggatggc gtggaatcgc tggccggtcc gtgcgtgcga
     2821 tgctccgggc gaccgccgct ggacctggcg ctggtactgc tgaccgtcgg ccgcgcggtc
     2881 cccatcgcgc aaatctttgg ggttcgcgat cttcgccaga acccgcttag gccggttgaa
     2941 tccatccggg tcctgcgcgc ccgcggggcc ttcacaacct ggtttcacga gggtgcggac
     3001 gccgatggaa tggtgacgtt tgaatatcca cggccgacgg gcaccgggac gattgctgtt
     3061 acgtatcggg aaatcgtctt ctacccgcgc tcaggggatg gcggaacgct gacgcgagga
     3121 gacagcggtt cggccgtcgt cgattccgac aatcgcctgc tgggcattca catcggcgca
     3181 agcactcgca gacagggcgc tgatactcac ctgctcggct atgcgcttcc gacgcacgtt
     3241 gcggcccgct gggcgtcggg tttctcgttg ctcactccag ccggtcccgt cgcctggcag
     3301 taggagaggt cccgtctcgg cattaccgcc cagtcatgcg cggccggtgg accggcaccc
     3361 tggatttcgc cgaaaggctt atcgtgaggg ctttcggtcc atttggcgcg ctatgataag
     3421 gttgaatttc agatgcaacc cctcagcatc gcatggcaag tcgcatttca gcagtgaaag
     3481 gaaccacatg cgcctgctaa gccctgcgtt cgcggagcgt gacccgatac cgaaaaagta
     3541 cacccaagat ggggcaaaca tttcgccgcc gttggtcgcg gttgacttgc cgcgaggcgt
     3601 tcgcgaatat gccctggtaa tggacgatct cggttcgccg cagcgccagc cacgcgtgca
     3661 ctgggtgatc tacaagattc cccctgaggt gcacgaattg ccggagaaca tcgcccggcg
     3721 agaagtgccc ccacggcctg tgggcgcgcg gcaaggtttg aactcctggc ggcataacgt
     3781 cggctatcgc ggcccggctc cgccttccgg tgacggcgtg caccactaca tgtttacgct
     3841 attcgcgctg gacgcgccgg tgcagttggc ctcaggtgtc agcaaaggcg ccctgttgaa
     3901 tgcaatccgc ggccacgtgc ttgcgcaagc aagccttatc ggaacttata aacgctagcc
     3961 tgcgaccgag gccgcattaa ggagaacggt tcggtgtctt gcagtcgccc ggctgagaat
     4021 ttggtcgaac tgccttcggc atcgagcgat acaataaatg cggaacggtc aggttggagg
     4081 aagcccgtgc aaactcaaat cgtgatacgc aacgccgagc agagcaatgg tctgcgcgcc
     4141 ttcgccgaag agaagttcgc ggaagcgctg gcgcgcttcg aacagaacgt gatgacggcg
     4201 gtcatgcggc tcgaggacat taccgggccg gacaaggaaa cggggcgtga caagctctgc
     4261 agcctgcatg tgaagctccg tacgggcgat atcgtcatca agcaagagca caccgacttt
     4321 cgcgccgcga tcgacgtggc gttggaccgc ttgaaagtcc aacttggccg cgaaatcggg
     4381 cgcgccaaac gcggcatcgc ggaaggctga agccgcaatt gaggccgaag ccgggatcac
     4441 tgggtggccc ggttcgaatc cggtaccatt gcaagcatga gtggcccggc tgacggcccg
     4501 ctgttgagcg taaaagattt gcggacctac ctgcctgccc ggcacggtgc ggggcgcgga
     4561 aagtcgtctc gtgaccgaaa cggctccggt gacgcggcgg acgtgatccg ggccgtcgat
     4621 ggcgtgtctt tcactctcga tcgtggccaa acgctcgggc tggtggggga gagcggctgc
     4681 ggcaaaacga cgttagcgcg cactatcctg cggctgattc ccgccacgag cggacaggtg
     4741 catttcgatg ggcgcgacgt gctggcgctg cgcggggcgg ccctgcgggc gatgcgccgc
     4801 gacatgcaga tcattttcca agatccgctc ggcagcctta atcctcgctt gcgcgtcgag
     4861 agcatcgtcg gcgaggcgct tactgtacat ggcctggtgc gcagtgccgc cgagcggcgc
     4921 gagcgtgtag ccgagttatt gcggcgcgta gggttgtcgc cggatgcttt atggcgctac
     4981 ccgcacgaat tttccggcgg gcagcggcag cgcatcggca tcgctcgggc gctggcgctg
     5041 cggcccaagc tgatcatctg cgacgagccg gtttcggcgc tggatgtgtc aatccagtcg
     5101 cagattttga acctgctcaa cgagctgcag gcggaatttg gcctgagtta cttactgatc
     5161 gcgcacaacc tggccgtcgt gcggcatgcc agcgaccgcg tggcggtcat gtacaagggg
     5221 cggatcgtcg agctcgctcc gacggacgag ctgttcgaca acccgcagca cccttacacg
     5281 cgcactctgc tggcggctgt gccgcgcgag gcatggggct cggccgtgca gatgtgaacc
     5341 tggtggggtt gccctcgccg ggctcgcatc agcgtgtcca ggctcggacc gcggcaaagt
     5401 ccccgccggt cgtaacccat tcgtagatgc gcgcgtaggc ccggtacgcc tgctcgcgtg
     5461 caaagctctg gcggagaatt tccggcagcg tgggcggctc aggatttgcc gcggtggcga
     5521 gcagggcggc agacagtgcg tccacgaact gcgtatttgc ggcgcggacc gagttgtaga
     5581 aacgatactc gtcgattgtg ccgagtgagc cgtacgggac gggaagcagg cggccgccgg
     5641 tgcgcgtgag caattcgcgg gccgcgccgg cgtccgtggc gatgatcggc aaaccggcgt
     5701 atagcgcttc ggccagggcc aggctccagc cctcgatgaa cgaaggcagg acgaacgcgt
     5761 cggccaggta gtagaacttc tgcggccgat cgtgatagcc gaggaaggtg actgcgggcc
     5821 ccacgccgag ccggtcgatt tcccggcgca cgagttgggc gtagtcggtt tcattcgggc
     5881 tgctttgggc ctggccgagc aaagcgagcc gcaaacgtgg atttttgcgg agggccgccg
     5941 cgagcgcccg caccaggaag agttgtgctt tgacttcgcc gagcgaagcg gtgcaaagaa
     6001 agacgaattc ctcgggcgcg aaacccaggg cggtccgctc ggcggcgcga tccaggccgg
     6061 agcgggcggc gtcgagggcc tgagtatcca caccgttcgt gacgactgcc attttgcccg
     6121 gcggcaatcc aaggcggagg tcagaatacg cggcgacggc gttcgagacg caggtatagc
     6181 cggtagtgtg ggcatcggcg gcgcgatatt cggcgatctg ctgaggcgaa agccagacat
     6241 aggtattgtg tattgtctgc atgaatggaa tacccaagtc cgccgccgtg ctcgcgccaa
     6301 aaagggaata gtgtgcgttg acgaggtcga tgccttcttg ccgaagcaag tcgcggtagg
     6361 cgctttcgcg agcagcactt tcagcgtcca ggcgcaacac gcgcaggccg cgctgccgag
     6421 ctttggctgc agccggtccc gctcgaccca gcacgaggag tgccggttcg aagcgctgcg
     6481 gatcaagcgt cgcggcgaca tccagcacca cgtgttcgag accgccctgg aggaaatctt
     6541 cgacctgcaa cagcacgcga gttcttcggc cggtcggcag atatgcgcgc ggcaactcgc
     6601 gccgggcgtc cgctttcgag attgtcttat tggtgaacag gtggttattg gcgtaagcca
     6661 gagccagatc gaccagcgcc ggccccggtg cagcgcgaaa cgccgcgcgc gtgagcaggc
     6721 ccagccgcgc caacgtcgtt ttgtcgtccg cgaagcaaac gtccgccagc gactgtattt
     6781 cgaccgcgac gtcgtaaggg ttcggctctg gcccggcgaa ccaggccgcg atgcagaggt
     6841 ccggcgagcg cgggtggttg gacaaactgg gcagagcatc agcgcggacg agaatcaggg
     6901 ttttgccgcc gtagtccgca ggaggctcgc tgcccagacg ctgcagcttg cagcgcgccg
     6961 gggtcaaagt gctcagccac gctgccgttg cttcatcaac gaggatggtc cacccttggc
     7021 ggtagtagcc ggtgcggccg gcttcgtagg tcatcagacg cgcggcatga tcgtgaattt
     7081 tgtgctccgc cgcgcgcgcg ttcagcgtgt tatcgtggac gcggtagcgg tatagaagct
     7141 cgtccgtgcc caggtggcag acggggaaca agctgttcat ccgcatccag tagtcgtagt
     7201 cttcgacgcc ttgccagggg agataatcgc ccaacaggcg gcccacccag ccgcggtaga
     7261 ggaacgaagc gccgataaaa ttatcctgaa cgacgttcag gttggccgtg ttgcgcggca
     7321 ggtgaatttc gggcgcgtcg ggactgcgcc ggttctgcgg ccgaaacgcg gggtcacgca
     7381 aaggctgccc gcggtcatct atcgcgagat agtcgctgta gaccatggcg gcttcggggt
     7441 gggcgcgcag gaagccgacc agcttttcga gctgggtagg ttccatcagg ttgtccgccg
     7501 aggtccaggt ccaaaactcc ccgcgcgcga agtcgaagcc gttgctgagc gctttgggca
     7561 gcttctggtt gggctgcgtg agcaggcgga tgcgcggctc gcgcaggtag ggctccaagg
     7621 ccgcggtcac gtcgtcggcg gacccgtcat cgaccacgat cagttcccag ttcgtgtagg
     7681 tttgcttcag gacgctgtcg atggactcgc gcagcaggcc agcttggttg tagacgggta
     7741 gaactatgct gacgaggccg ggcgcctggc cgggaaatgc cgggtctgtg cccggaccag
     7801 tcgtaagggc cacggacgcg agcggcttgt gccggtagag cagccgccgg cccgccagcc
     7861 gcagcttgcg gcccagggcg cgcgtcagcg cggaggggcc ttgctgcgcg tgaaagacga
     7921 agcggcgctg gttgtgaagc agccagcgcg ccagtctttc gcgccggctg ccgcgcggga
     7981 aagcggcata tcgcaggcgc tgggcggcgg aggcgtcgga gttcttgcct ttcgaattca
     8041 tggctcgcgt tgggcgacac tgtacccgat ctgtgcggcg ggtgccccgg ctccgtcact
     8101 tgcggcgcag aaacgtgcct gccctccctt ttcaacgaga gccggcggca cgatcatgaa
     8161 agccgtctcg ccggttacgg ctcgaaacgt caaagggaga acttatgacc gccaaggacg
     8221 ttatcaagaa cacgttggat ttttgccgca tggtcacggt cgagtacctg aaggacttcg
     8281 ccgatgcgga cttgctggtt cgaccggtgc cggcggcgaa tcacaccgcc tggcagttgg
     8341 ggcacctgat cacctcggaa catgagatga tctcagccct ggggcacaaa atgcccgagc
     8401 tgcccgctgg ttttgtagag cggtacacgc gagaaagcgc caggtcggac cagccggcgg
     8461 agttcgcgac caagcaggag taccttgatc ttcttgagaa ggtccgggcc ggcacgctgg
     8521 ccgcactgaa cgcgacgccc gaggctgact tggagaaacc cgcgccggag tcgatgcggg
     8581 cctacgcccc gacggtgtcg gcggtcttcc tgatcaccgg cacacacgag ctgatgcatg
     8641 ccggtcagtt tgtgacggtg cggcgaaagc tgggcaagcc ggtgctgttc taaccgcaac
     8701 gatttgcgga caaaaacacc cgcgaattcc ggggcgatac gcccgcgttc tgccccttcc
     8761 cgccagcgct ctggcttgac gcttcggccg gtgcgccgtt accttccggc tttcacgttt
     8821 gaggcgtgag agtgggagtc caagcgtgcc ctacgactcg attgtgtgcg taaaacaggt
     8881 gcccgacacg gccaatatca ccggcgaggc gatgcgccct gatgggacgg tcaaccgggc
     8941 ggccctgccc acggtgttca acccggacga cttgcacgcc ctggaaatgg ccctggcggt
     9001 gcgcgaggcg catggcggaa ccgtgaccgt gctcaccatg ggcccgccca aggccggcga
     9061 ggtgttgcgc gaggccgtgt accgcggcgc cgaccgcgcc atcctgctga ccgaccgccg
     9121 ggccgcggcc agcgatacgc ttgccaccag ctacattctc agcatggccg tccgcaaaat
     9181 cggccgctat gacttcgttt tctgcggccg gcaggcgatc gacggcgata cggcccaagt
     9241 cggcccgcag acggcggaaa agctgggcat cccgcaggtg acttatctcg aacaagtggt
     9301 ggacctgcac gagaacatcg cccgcctgcg gcgcaacgtc ggcagcggct gggagatcgt
     9361 cgaggtccgg ctgccggtgc tggtgaccgt gctggataca gccggcctgg ttcggccgcc
     9421 ggccgccaag cgcgtgctca agtacaagcg ggcccgtacg ccaagcgagt tgttgatcga
     9481 agtgtcgcgt gagaaccccg atttcagcga ccagcaaaag gaagccgagg ctctgcggcg
     9541 ggccgagtcg ctccgctgcc gcgggctgat gcttgaggaa tggaatctgg atgacatcgg
     9601 ggcggacttg gcctggtgcg gtctgagcgg ctcgccgacc aaggtccacc gcgtgcagtc
     9661 gatcgtgctg acgaagcagg gctacacaga aattccaccg accgacgacg gcattcgcgg
     9721 cctgatccgc gaactggtca ttgaccacac gctaggatga gaattaacca caagacacaa
     9781 aggcacagag aagaattaag gtgaaaagtt gtatctatgt cttctctgta gctctgtgcc
     9841 gctgtggttg tttttagttt gaggaaagca tgatcccggt gaaccgcagt ggcgaggtgt
     9901 gggtttttgc cgagcaggaa gagggcagtc tgcatgacat cgcccttgaa ctgtgcggca
     9961 aggcgcggca attggccgac cagcttggcg tcaagatggc ggccgtgctg tccggctgga
    10021 atgtgcgcga gctttcgcag cggctgttcg cgcacggggc cgatcgcgtg tacctggtcg
    10081 aggaccggcg gctggagcac taccagacgc agccatacgc caagatcctc tgtacgctga
    10141 tagacaagca ccgcccgcag attgtgattt acggggcgac gccgctgggg cgtgatttgg
    10201 cgccgcgcat cgcctcggcc atgaaggccg gcttaacggc tgactgcacc gcgctggaaa
    10261 tcagcgacgt gaccgatccc aagacgcagc aggtgcacaa gaacctgttg ctccaaatcc
    10321 gcccggcctt cggcggcaac atcatcgcca cgattgtcaa ctgggaccgc tggccgcaaa
    10381 tggcgaccgt gcgcgagggc gtcatgccca tgctcgagcc ggacatgcaa cgcacgggcg
    10441 aaattttaga agagcaagtt gccctgactg acgcggactt tccgctgcgc ctggtcgaac
    10501 gccatcgcga accgcgcaag gtcaacctta aaggggcgcg aaccatcgtg gccggcggtg
    10561 gcggtgtggg cagcaaggcc aacttccgca tcattcacga attggccggc gccattggcg
    10621 gggcggtcgg cgcctcgcgc gctgcggtgg atggtggcta catcggcaag gagcaccagg
    10681 tcggccagac cggcaccacg gtgcgcccgg ctctctatat cgcctgcggg atcagcgggg
    10741 cggtgcaaca ccgggccggt atggaagaat ccgccaagat cgtcgctatc aactgggaca
    10801 aggaagcccc catcttcggc gtcgcccact atggcattgt cggcgacctg aacgaggtta
    10861 ttcccaagat gatcaaggcg ctgaaggagc gtccgaagga ataaggcaag ccggggccgt
    10921 cccggaggta tgcagcgtgg cctcgccaaa tgggacggat aaaaacgcgc gatcgccttt
    10981 cctggtcggc gagcactggt cggcaaagaa cgacgtgacg ctgtttgcgg gggattgccg
    11041 ggaactgctg gcgaggctgc cggcgcgatc ggttcagctc gtcgtcacgt cgccgccgta
    11101 caacattggc aagacatatg agcggcgggt ctcactgccc gactacctgc agcgcgaacg
    11161 cgaagtgatc aatctgtgcg ccgacaagct gcgggcgggc ggcagcatct gttggcagat
    11221 tggaaaccac gtcgccgagg gcgagatttt cccgctcgat acactactgt atccgtattt
    11281 caaggagcgc gggctgcatt tgcggaaccg gatcatctgg catttcgagc acggtctgca
    11341 ttgttcgcgg cggttctccg ggcggtacga gacaattctg tggttcacga aagacgatgc
    11401 cgcgggacag tactacttcg atttggatcc ggtgcgcgtt ccgcagaaat accccggcaa
    11461 acgccacttc aaaggaccgc acaagggcga gtactcggcg aacccgcttg gtaaaaatcc
    11521 gggcgatgtg tggattatcc cgaacgtcaa acacaaccac ccggaaaaga cggttcaccc
    11581 ctgtcaattt cccgttgagc tcgtcgagcg cctggtgctg gcgcttaccc gccgcgggga
    11641 tttggtcctc gatccctaca tgggcgttgg aacaacaccg gcggcggccg tcgttcatgg
    11701 acggcgcgcc gccggtgctg aaatcgtgcc tgagtatgtg aagatcgccc gcgagcgggt
    11761 cgcgcaggcg gtcagcggta cgctgcccgc ccggcctcgt tctcgtccgg tctacgtgcc
    11821 gtcgcctaat tcgccactgg tgaagcggtc ataagtggcc gacgactcgc cggctgtttg
    11881 cgccgcagcg gttaggctgc gtgagccgga gggccggtga cctccggctc acagttcgtg
    11941 acactttgat cgaagattac tcgccgtact tggcgataat ctcttccttc gtcttgtgca
    12001 gaatgtcgta cttcttgcca accttcgtga acgcctcaat cgtccggtcg atgtggctga
    12061 agtcgtgcgc ggctgacatc tggcagcgga tgcgggccag gcccttgccg accaccgggt
    12121 agaagaaccc gatcacgaag atgccttcgg aatacatgtc gcgggcgatg tcctggctca
    12181 gcttcgcgtt gtacagcatg atcggaacga tcgggtgatc gcccggcttg atgtcgaagc
    12241 cggcttcctt catgcccttg cggaaacgct tggtgttcgc ctcgaccttg tcacgcagct
    12301 cggtcgactc cgtgatcatg tcgaacaccg cgatcgaagc cttcaccacg atcgacgaaa
    12361 ccgtgttgct gaacaggtac ggccggctgc gctgccgcag catctcgatg atttccttgc
    12421 ggccggtggt gaaaccgccc gaggcgccgc cgagggcttt gcccagcgtg ccggtaacga
    12481 tgtcgacttt gcccatgacg ccgcagtact cggttgagcc gcggccggtc ttgccgatca
    12541 cgccggtgca gtgggcgtcg tcgaccatga ccaaggcgtc gtacttctcg gccagggcga
    12601 cgattttgtc gagctgggca atggtgccgt ccatgctgaa ggcgccgtcg gtggcgatca
    12661 cgcgatagcg gcagttctgc gtttccttga gcttggcctc gaggtcggcc atgtcgttgt
    12721 ttttgtaaat gtgacgggtg gccttgcaca ggcggatacc gtcgatgatc gacgcatggt
    12781 tgagggcgtc ggtgatgatg gcgtcgtcct tgccgaagag cggctcgaat acgccgccgt
    12841 tggcgtcgaa gcaggacgag tagaggatcg cgtcttcggt atggaagaag tcggcgatct
    12901 tccgctcgag ttccttgtgg atgtcctggg tgccgcagat aaagcgcacg ctgctcatgc
    12961 cgtagccgtg ggtcttgagg ccctcgtggg cggcctcgat cacgcgcggg tggctggaca
    13021 ggccgaggta gttattcgcg cagaagttga tgacttcctt cttcggcgcg cccgggggat
    13081 actccacctg cacatcggcg gcctgcggcg tcaggatgta acgttcttcc ttgaacaagc
    13141 cagcttcacg aatgctgact agttccttct gaaggtcttg cttcatcttg ccaaacataa
    13201 tctctcgctc caattcgccg ggctgttccg gcaatctgta ggtcgggtca accgacccga
    13261 cgcacccttt gataacgtcg ggtctgcggg acccgaccta cggttgttgt gtattgtgag
    13321 accggttttc aaccggtcat gctcgtcgga caccggtcga ttacgtcggc cacggggggc
    13381 cgacgctacc cggggggcct acttctggcc gcggcgcttc atggccgcct cgtattcgct
    13441 gcgatccggg aacaacacca ccttggcgct ggtccgcgga cgctccagca tcttattgaa
    13501 gcccgcttcg aattcgctca gcgggaaaat atccgtgatc accggcgtcg ggtccagccg
    13561 cccgttggcc agcaggttcc ggttgcggta ccacgtgtcg aaaatcttgc ggccgttgat
    13621 gccgtggatc tggcaggctt tgaacacgat gccgttgttg tagtcgaccg gcagccggtt
    13681 ctccgccgct acgccgaagg ccgtgatgcg ccccgccttg cgcacgcagg cgaaacagtc
    13741 atccacggaa tcctgcgagc cgaccatctc gagcgcgatg tccacgccgt tcccgccggt
    13801 ctggtccatg acaaactgga accgttcctt ggccgtggtc ttgttggtgt acatctgatg
    13861 atcggcgccc atcttcttgc cgatctgcat gttcacgtcg tacttgccga agtgaatgat
    13921 cttcgccaca cccaccgagc gggccacgcc gatcgcgaac aggctgatcg gaccatcgcc
    13981 ggtgatcagc atgctcttgc cggccacgtc cgcatcctcg cccagcaccg cgtaggtcgc
    14041 gttgccgagc ggctcctgga tggtcgcaac ttcgggcgga atcgacggat cattcggcca
    14101 gcagatcgat tcgggcagcg cgaagtattc ggcgaagcag ccgtcaaaat cgacgcccag
    14161 aatcttcatg tgctggccaa catgcggcga gccgagcatc gtggtcagat cgccgggatc
    14221 gaaaatgtgg gtctcggcgg agatataatc gccgactttt atccgcttgc agtgttcgcc
    14281 aacctcaacc acctcgcccg cgacctcgtg cccgaggatt tgcggcagct tggcggcgcc
    14341 gatccggccg gcagaccagg gatcccactt gtaaatatgc acgtcggtgc cgcaaatact
    14401 tgtggcacgg acctttacga tcacccaatc gcttttgggt tgcggaaccg gcttgtcctg
    14461 catcacggcg ccgaactccg gcttcgtctt gaccaccgcc ttcatggtcg ccatcaacta
    14521 tctccttgcg tgaaactcaa ccgccagcgt gtctgtatgg ggaatttgac gcgagccaaa
    14581 ccgcgtcgcc atcgccgcca aggccgagtg tgcgttttat cgcaatccgt gttccagccg
    14641 cacgggcccc gctcggccta ctaaggtcaa aactggccct ggatcaatca gtttaaggcg
    14701 cgcctggacc gccggcaaga atccggctgc ggtggtgcga ggttaaggtg ctgcagggag
    14761 tgggagccgc ggggcgtggg tgctgagcgg ggttgcgtat tcaagcgtgg gtgccatgcc
    14821 caagcccgcg aagcgggcgc gggcatgccg tttccagcag ccccacgctg gcgctgttct
    14881 gacgccgtga ggacggcctg cccgcgcgta cagcttgggc atggcgccca acccacccgg
    14941 acttgttaac gcctacccgc gtttgcagct tcatccggcg cccgccagac gtggcccgcc
    15001 agagctaaca ggtcctccga acgcccttcg gaaaacgcag gtcaagcgga attttttaac
    15061 gaatgcgact ctttttttcg cagaggccca taacccccgc tcttcctgcg gaagcggccc
    15121 gctaatcatg cgcgccgggc gggctgcgca cccccgactt cgggacctgc ccgcgggcgt
    15181 cccaattccg cggcacgagc cctccacgcc cgacaaaatt cgtcctgagc gttggcttcc
    15241 ggctcccacg ttttgaacgc ttccctcggt tcatacccgc gacaggcaaa cgaaacgaag
    15301 gaggatcggc atggcaatgt cagtgaccga caacaacact ctaaccttgc ttaacacaat
    15361 ggattaccta tctgcccaac agggcgctat tttcagacgc ctctgcactg gttcgaagat
    15421 caaccgtggc gcggacgacc ccgccggctt gatggccctc caatccatca attgcgagct
    15481 gatcggcgtg gatgccgcca ttcggaacaa ccagcgcagc gacgccatgc tgaacgtcgc
    15541 ggactccgca ctgacacagt tgagcgacct gttgggccag attcagaccc tggtcgcaca
    15601 atcctcaaac tcggccggtc tgactgccaa cgaaatcgcc agtaaccagt cgcagattga
    15661 cgacgcgatc gcttcgatcg aacgtatcgt gcgcaccacg gagttcaatg gcagaaatct
    15721 gctcgacggc tcgctgggca tcaacgtgac cggcgccaac gcgacctatt tcgagaacgt
    15781 gcgcgtctac agccgcgacc cgagcgccgc cagtaatgcg gtccgtgtca cagtgaacgg
    15841 cgtggcgcag cgcgccaaag cggagggcct gaccacaacc gccgcgtccc aagccacgac
    15901 catcgaagta aagggccgcc tgggtacgca gatcatcgat ctcaaggccg acgagaacct
    15961 ctcggctgtc gtagcgaaaa tcaacgacgt gaccgccttg accggcgtat acgcctccgg
    16021 cacctcggcg ggcgtggatc tgtacagtaa gggctacggc tcggccgagt tcgtcaaggt
    16081 cagcatcctc tccggtgaca cgacccactt caaagagctg gacggggcgg gcgtggacgc
    16141 cgacgtcacc gtaaacggcg cggccgcctc ggccgatggc aacgaagtct acttcagcca
    16201 gaacgggttg aatctggcct tcaggatcac agaagcgttt accgccggca cgacgcagac
    16261 gttcgaggtg gacaaaaccg gcggggctac cttccaactt ggtacggacg caaccacgcg
    16321 ctacaccttg ggcattgaca gcctttcccc gcgccgcctg ggcggcaaga gcgcgggcgg
    16381 attcctgtca gacctcaaga gcggcggagc agcctcgctg gcgaagaacc ccgccgctgc
    16441 ggcggccatc gcggccgacg ctcagaagca ggtcgcgacc atgcagggcc ggatcggcag
    16501 cttccagaag taccagatcg aaatctcgaa gaacaccatg gcggccatca aggagagtct
    16561 gaccaccgtc aagagctcaa ttgctgatgt ggattacgcg caagagacgg cggcgctagc
    16621 gcgtcaggac gtgctgatgc aatcggtagc ctccatgctc agcgtcgcca gtcagcagag
    16681 cgctcagcga attctgagcc tgctgtaagt gttcgagccc gcgcggtccg gcgagcgggg
    16741 attgagcatc caaccgagcc gggcagcgcc caaccgcccg gctccggctc gctacgctcg
    16801 gaagtggccg ctcctggcgc tgaatctggc ggtcaactcc ctttggttac tcgctgccgt
    16861 cggcttgtca cggggcctag ggcagcgggg agacgcgcca cccgctgagc gatgactcgg
    16921 cgggcgacgc gctcctgcgc ccgtgggcta atgcgaaaga aactggccca tcaacgtgtg
    16981 gccggtgtca accatgactc cggtgacggc ggccgccgcc tcggagaact gggccgcgtt
    17041 tgaaggcgtg atgccgtggc cggccatggc gaacggcgtg ggcgtggcgt catgggcagt
    17101 ggtcgtgcac ggcgtgtagt ggtccgggac gaccaggatc cgccactcgg caaactggcg
    17161 cagcttctcc agcagtggcg cgacgatgtg ttcgtcgatg cgctcgaggg ctttcacctt
    17221 ctcggtcacg ttgcccgcgt ggccggcctc atccgttgct tcgacatgga ctgcgatcag
    17281 gtcaaagtca tccagcgcgc ggaccgctgc gcggcctttc ccggcgtagt ccgtgtcgaa
    17341 atagccggtc gcaccgggaa cgtcgatcag ctccattccc attgagatgg ccagcccacg
    17401 aatgatatcc acggcagtga tgacggcggc gcgcaggccg aagcgctcgg cgaacggctc
    17461 gaagcgccgc gggcgcccct gcccccacag ccagatgtcg gagacggggt cgcggcccgc
    17521 cgcgcggcgc gcctggttga ctgggtggtc cgccagaacc tccgcgcccc gctccatgat
    17581 tttcaatacc cgctcggcgc ccgggccctg tggcagatgc ccgaagacgg gctgattcgg
    17641 aatgtcgtgc ggcggcgtgc atttcacgtc ataagcggcc accggcgagg ccagcatcag
    17701 gttccgatac ccaacgccgg cgtggaactt gcaccccgct aaggccggat cagacgcggc
    17761 cagcgattgc agctcagcga ttaactggtc ggcctcgtcc tgggaaatgt gcccggcggt
    17821 gaagtcgcac atgcggccgc ccaggatcgt gacgaagttg cagcgaaaaa tgacctcatc
    17881 gggcccgatc ggcagccccc gggcggcggc ctcaatcggg gcgcgcccgc tgtagtacac
    17941 gtgcgggtca tagccgaaca gggacatgct ggccacgtcg gtgccgggga taaacccggc
    18001 gggcacggtt aggactcgcc cgaggcgtcc ggtgcgggcg atcgcatcca tgtttgggat
    18061 gggcgcggcg gccagcggcg tccgcccacc gagctgcggc gacggaacgt cggccgcccc
    18121 gtccggcaga attatcgcgt atttcatcgg cgctccttct gggcgaagcg ctcatgccgg
    18181 caggcaatca tgccgcctgg cgctggagaa gcgcatcgct gatggaatct taccggctcg
    18241 gcggcgtttt ttgactcccg ccaaacaggt caacgtggcc gttcgcgccg cacgcaagaa
    18301 gggtcggcag gcgtttctcc gccagcgcgc tgaaaagctc cggcccttct tcagagctgc
    18361 acccggcgca ggcggagggc gttgcccact acggaaaccg agctgaagct catcgccgcc
    18421 gccgcaatca tgggattcag cagcagcccg aacacgggat agagcacgcc cgccgcaatg
    18481 ggaatgccga tggcgttgta aatgaacgcg aagaacaggt tctgccggat gttgcgcacc
    18541 gcggcccggc tcagcagccg agctttggca attccgcgca aatcgccctt caccagcgtg
    18601 atgccggcgc tctccatggc gacgtcggtc cccgtgccca tcgcgattcc cacgtccgcc
    18661 tgggccaaag ccggtgcatc gttcacgccg tcgccggcca tcgcgacttt tcggccttcc
    18721 tgctgcaagc gcctgacgac cgccgccttc tgatccggca gcacctccgc ctctacgcgc
    18781 gtcaacccga gcttgccggc cacggcggcg gccgtcgtgc gactgtcacc cgtgaccatc
    18841 acgatctcaa cgcccgcgtc gcgcagcatc cgcacggcct caggcgtaga gcctttgatc
    18901 ggatcagcca cgcccagcag gccggccggc ttgccatcaa cggccacgaa catgaccgtt
    18961 tgtccatcgc gccgcaattc ttcggcccgg gcgagcatcg cgctcgtggc gacgtgcagt
    19021 tgttccatga aaccggcgtt gccgagcgcc atggcccgcc cgttcacgcg cccccttacg
    19081 cccttgccgg tcaccgactc gaactcttgc gcctcggccg gctcgatgcc gcgctcagtt
    19141 gccccgctga cgatcgcggc cgccagcgga tgctcgctgc cgcgctcaat gctggcggcg
    19201 aagtgcaaca ggtccgcctc ggtccagccc gcggccggcg tcagcgagac cagcttcggc
    19261 ttgccttcgg tgagcgtgcc ggttttgtcg acgatcagcg tgtcgatgcg ctccatcact
    19321 tccagcgcct cggcgttcct gatcaacacg ccggcttgcg ccccacgccc cacgcccacc
    19381 atcacgctca tcggcgtcgc caggcccagc gcacacggac aagcaatgat cagcaccgcc
    19441 acggcgttga gaatggcaaa agccatcgcc ggggctgggc cccagatgct ccagacgaca
    19501 aacgtcacga tcgccgccag gatcaccgcc ggcacgaacc agcccgccac cttgtcggcc
    19561 aagcgctgaa tcggggcccg gctgcgctgc gcctcgccca ccatctgtac gatgcgcgcc
    19621 agcagcgtgt cgctgcccac ccgttcggcc cgcatgatga agctgccggt cgcgttcacc
    19681 gtgctgccga tcactttgtc gcccggctgc ttctcaaccg gaatcggctc gccggtgatc
    19741 attgactcat cgacggcgct gcggccctcc aacaccgtcc cgtccaccgg caccttctcg
    19801 cccggccgaa cgcgcagccg gtcgccgata tgcacctcct gaagcgggat gtcctcttcc
    19861 gcgccgtcgt cccgcagtcg tcgtgcagtt ttggccgcca tgcccagcag cgcccggatc
    19921 gcaccgctgg tactgcggcg cgcccgcagc tccagcacct gcccgagcag caccagggaa
    19981 gtaatgaccg cggccgcctc aaagtaaacg ccgaccgctc cgctttcgtc gcggaaggac
    20041 gccgggaata tgccgggagc gacgacggcc accaagctgt acgtccacgc cacgccgacg
    20101 cccatcgcaa tcagcgtgaa catattaagg ctgcggtgta ccaccgattg ccagccgcgc
    20161 accagcaacg gccagccgca ccacaggacc acgggggtgg cgagcgccag ttcgatccat
    20221 ccgcggagcg ccggactgag ccaatgtccc agacccggaa ttgcatgtcc catggcaatg
    20281 accacgagcg gcgccgtcag cactaagcta atccagaacc gccgcgtcat gtcgcgcagc
    20341 tccgggtctt cctcttcttc cacggacacc gtcagcggct caagggccat gccgcagatt
    20401 gggcaactcc cggggcgatc gctgatgact tgcgggtgca tcgggcaggt gtactggacc
    20461 tgggcaggcg ccgcggctgg cgtcaggcgc tccagcgcca tgccgcattt cgggcacgca
    20521 ccgggcttcg cctggtggac ttcggggtgc atggggcagg tatacggacc cgtttccgcg
    20581 ggccggtgtt ccgcatgcgg ctcggccggc gcctcgccct tcggttgcga cgcggccgta
    20641 cccccagcct cgcgtcgcgg ctcggcggca gcctcgccct cgcgtcgcgg tggctggccg
    20701 gcctgtggag ctccatgctg aacgggcaac gcgatccgtc ccggccccgt cggcgcaggc
    20761 tgtggcgcag gctgctcgga agtgaaccgg cgcgcatcgc ggcgaaaagc ctccaggcaa
    20821 tgccggcagc agaagtagta agtccggccc tcgtactcgc agcgattcgt cgccgacgcc
    20881 gggtcgacct ccattccgca aacgggatct ctcacgcgtt gacctccacc ggccacttat
    20941 tgacctccgc aatccttccg gtgtttccca aagctgtaag cgcgggcatg ccttcgccat
    21001 aggtggaggc gacttccgat tccggctcgg caccaactgg ctccggtctg gcacgaactg
    21061 cctccgattc ccgcttggcg ccaacaggct tcaattccgg cttgacacga gccggctgtg
    21121 ctaccgcggc gggtggcgcg gttagcgtgg gtgccatgcc caagcccgcg ttgcgggcgc
    21181 gggcatgccc gccccggggc ccacaatgcg atgttaccaa ctgttgctga ctcaccggca
    21241 atctccggcg caactcgaac tgatgatggt atctccgaaa cgctccccac gcccggccgg
    21301 cggctcgcga gcaaacacgc acgcgtcacg tacatcggca acgccgaggt acgcaaaatc
    21361 atgccccctt atttgcaggg aacgccggaa aataaacgga cgagtgttcg caaatgtcta
    21421 tttctcaagc agctacggaa atttcaattt tcacggcttc ggcctggggc ccgcccggtg
    21481 cgcgacgcac cgccaccaca gaatcacaac ggaagcgcgt agttcgccag gctaaaattc
    21541 cgccaaattt ggacggcatc gtatgacagc ccctgcgcca ccaggcaccg catggcctcg
    21601 cgcggagaaa ccataggtat tctacggcac gggcgaaagt gcctcttttt cccgacaaat
    21661 tccgtagttt tgtcgcggct tggcactcgc tgacacccgc cgttagaatg tggcattgac
    21721 cgtttgccac cgtagctcaa tcggcagagc agcggttttg taaaccgcag gtttcgggtt
    21781 caagtcccga cggtggcttt ctgtttcggg caccgaaaca gcggccggtt ttcgcgaaag
    21841 tcggccgtta attccggaac gtgcagcctg cgagtggctg ggtggtacca aatcggtggc
    21901 ttctgggccg aggccgccga cagatgttct gacggagaat gaaaatgatc gagagaagcg
    21961 gcgtaactac gttccagggc aaccctctga cgcttatcgg accggcgctc aatgcgggga
    22021 gtgcggcgcc ggagttcgtg ttgacggcca acgacctgtc ggacctgcgg agcgacgcct
    22081 accgcggcaa gctcttggtg ctcagcgtgg tgccgagcct ggatacgcct gtctgcgcga
    22141 cgcagacccg cacgtttaac cagcgggcga ccgggctggg cgaggacgtg gtagtattga
    22201 cggtcagcat ggatttgccc ttcgcccaga agcgattctg cggggcggaa gggattcagc
    22261 gcgtggtcac ggcgagcgac tataagcacc gctcgttcgg gccggcctac ggggtgctga
    22321 tcaaggagct ggggctgctg gctcgggcgg tattcgtggt cgaccgcgcg ggcaaggtga
    22381 cgtacagcga gtatgtgccg gaagtcacgc aagagccgaa ttacgatgcc gcgctggagg
    22441 cgctcaaagc ggcgaagtga tccgggattc agggatcgag ggttcgcggg atcaagaggt
    22501 gcgctgtcgc ggcaggtggc gtgtcgcggc agagagcaga gccggggcgt gtcgccccgg
    22561 aatgatgcga tcgggtgccc tcgctggcgc ttcgggctcg gatcaagggg gcccggatca
    22621 cggtggctcg gatgtcgctt cgcgctcgga tttcgcttcg cgcagaaagg gtcgggttgg
    22681 acccgagaag tcgcgccttg ccctacagat cctgaaggac ttcgaagaac tgcgggaagc
    22741 tttttgaaac gcagcgggcg ttgcggatgg tgatgccttc ggccgccagg cctgccaggg
    22801 cgaagctcat ggccatgcga tggtcgttgt aggtttcgat ggttgcgggt tggacgcgcg
    22861 gcggcgggta gatggtgagg ccgtcgtcgc gctgctcgac gcgtgcaccg aggcgggcca
    22921 gctcggtaac cagggcgccc aggcggtcgg tttccttgag gcgcaggttg ggcacgttgc
    22981 ggatggtggt tgggccgtcg gcgaagaggc tgatgacggc cagggtctga gccgtatccg
    23041 gcatggggtt caggtcgacg tcgatgccgc gcagccggcc ggcggcgggc ccgctgacct
    23101 ccaaaaaatc gggcccggcg ctgatgctgc agcccattgc ctctagcaaa tctacgaagt
    23161 gcacgtcgcc ctgaagactg gcacgagaca gcccgtccac gcgcacggcg ccgcccgtga
    23221 cggccgccgc gccccagagg tacgtggcgg ccgaggcgtc cggctcgacg gcgtattcac
    23281 cgccgcgata gcgttgcccg gcgggcacga tgaagcggcc cccgtcgccc agcacctcca
    23341 cgcccatctg gcgcatgagc tgaacggtca tctcgatgta tggctcgctg ggcggccggc
    23401 cgcgcaaagc gataaagcag tcgctcatgg cgtaaggcgc gaccatcaaa agcgcgctga
    23461 cgaactggct ggaggccagg cgatccagtc gcacgcgccc gccgaccagg ccccgcgcca
    23521 tggcggttac cggcgggaag cccggttcgg cgtcgtatcc cagcggcgca cccaggccct
    23581 ggagtgcgcc gacgagcggg ccgattgggc gttgccgcat gcgcggcgag ccgtccagcc
    23641 ggtatcgccc gtagccgagc gtaatcaggg cggtgaggaa acgcatcacg gtccccgcat
    23701 cgccggcgtc gagctcggct tcgccggccg gaaggtgacc ccgacagccg gcgaccgcga
    23761 tctgcggcgg gttagtgtgg cggatgccga ttacgattcc gagttcccgc agacaggtaa
    23821 ccatgcgatc ggcgtcggca gcgagagaag ctccgtcgag gatggtctgt ccatcggcca
    23881 gggcggcgca gagcaggtag cggttagtga ggctttttga gccgggcggg cgaacgcggg
    23941 cgcggatcgg gccggtggga tgaaggtgga catctgcccc gctccatgtc acgcgcatag
    24001 ccgcctccgg caagccttgg tagcaagctc cggcagatct tagcacgctc gggcggcata
    24061 gagtgataaa gtgataaagt gatagggtga taaagaggcg tcctttatca ccctatcact
    24121 ttatcacctt atcacgtcat cgcgggcgca cgcggcccgc tactgcttga agctgcgctc
    24181 gtggcgagac tggagctcta cggcgacgct actccaggca gggcgagaac ttcgttgcgg
    24241 atccaactca cgcgcgagtc gttctcctgg tcggtttcga tcacggtcac caggtcatag
    24301 tcgccgtccg cgtccaggtc gccgtacccg agccagccca cgtcgccgcc cgcggggaaa
    24361 ttcgtgatga ctaccggatg ccacacctgc gtcggatcgc cggagctctc gaagtagcga
    24421 acggcgcccg cgctgccgcc gacaacgatc tccaaggcgc catcgaggtc aatgtccacg
    24481 atcttcaagt cgcgcggctc gaaggtttcg aaggtcacca gcacgtaggt gtcccagtcg
    24541 tagctgcggg ccggcccggc gttcggctgg acgaacagca acatctgttg ctgattgctg
    24601 ccggtcgcga ccacgtcggg gcggccgtcg ccggtcaggt cagcgatgtc gatgctccag
    24661 gcgttgcgga cgctgccgat gcgccactgc ttccagggcg tatcgaccag cagaccgggg
    24721 cccggggcct gcggccggtt ttcaaccggg ttttcgaacc acgccacctg cgcgttgttg
    24781 tcttcaccgg cggccgaaat gacgtccaaa tcgccatcgt cgtcgagatc gtacagcagc
    24841 aggccggtcg ccccgtcgcg atcgagccgg ctctggcgct cgtgggtgcc gaggctgatg
    24901 gcggtccagt tgtggccggt ggtggagaag ccggggttta tgaaggccat cacgttgccg
    24961 tcggtgattt gaatgggttc gacgggaaca tcggggcgcg gcgtgcaggt cataacgaag
    25021 ctgcgcgagg cgatcacgtc gttgtcgccg tcgttgtcca cgtcgccgat ctggacgttg
    25081 cgataccttt gctccacggt gacgatgtag tcatcgatat tgacgcctgg gccgagggct
    25141 tgggagatga ttgcctggat ttcggagtcg gacagcatcg tgtaactggc gtcgatccgc
    25201 tcgcgcaact catcgttcgg atcagggttg ccccagtcgg ccagagcggt agtgggcaaa
    25261 ccggtggggt gatggaaata ccagactgcg ccctcggcgc tggcgacgat gtccaaagcg
    25321 ccgtcgcggt cgatgtcgcc gaccgccacg tcggtcaggt tccgcatgtc gcgtttgctg
    25381 tcgagcgtga ggctggtgta gcggacgaat ccaggcagcg tgtccggatc actgagcaga
    25441 atttgaatga cggcttgctc gttgccgtag ccgaccacgg ggtcgatttt gccgtcgccg
    25501 ttgaagtcga tgccgtaagg ggtgcgcgac aggacgtcgc cgtcggggat gaggtcggcg
    25561 ccgtcgagat cgttcaacag gtaggtaaca gtcgtaactg cggtgccatt ggtgaaggtg
    25621 cgggcctgga cgagttcctc cgtgaacgcc gggccgccgc cggtgtagct gccggcgcct
    25681 tcgcttttgg gtcttgtcca gccgccgcca agccactcgg gtatgtactg cggctcgcag
    25741 gcgaagagga ggcacgacaa tccgataaac agcgcgggcg gggcagtgcg cgaaaggtag
    25801 cgaagcattg ggtgactcca tgcgcggccg gccggtcaat caccggaagg agcgtccgct
    25861 cgcttatcgg gtcggctgat tgagagaggc gctttagggt ctcttttcga gggcccaggc
    25921 tggccggggc gtcagggggt ggcggttagc gtcgcgtggc cgacgggcag gttttgtacg
    25981 tgcgaaagca cttgttccgg cagtaagccg tccaggcagg cgtggtgacc gagcgggcat
    26041 gtgacccgat aacacggggc acacggcagg gcgcgcgaaa ccacatgtgc ccgcgggctg
    26101 tagggaccgg tgcggtgcgg attggtcggg ccgaacaggg cgaccagcgg tttgttcagg
    26161 gcggcggcaa tgtgcatggg gcccgagtcg aggcaaatga cgaagcggga aaggtccagt
    26221 aaggcgacga gttggggcag actggtgcgc ccgacgagat tcactatctc cgctttgcag
    26281 gcggcggtga tgcgctgggc gatgcccgcc tcagaaggcg ctcccagaag tacacaagtc
    26341 ggttggccgt cggccgccag gcgttcgatc agctttgcga aatgtgattc gttccacagt
    26401 tttgacggcc agcgcgccgc gggcaggatg gcgacgaatt cgcgcagctc gcggccggcg
    26461 gccgctgcca gcttattgcg ggcttgctcg atttcatgtg gcggcacatg caatggaaat
    26521 tcgggcgcgg ccggcgcagc tcccagcgcg gccgcgattt gcaggttttt gtcgaccgcg
    26581 tgcacggcgg ccggcgcgca gcgcacccgg cgtgtgtaga acagcggcgc cagctcgcgg
    26641 gccacggcga agccgacgcg ttgcggggcg ccgctagccc aagccagaaa gccgctgcga
    26701 aacagcccct gcaagtcgat taccaggtca aagcgccggc ggcgcagcgc gaatacgaag
    26761 cgcgcgaagt cggccagggc gcgcgggctt cgtagcaggc ggccgtaacg ccggcggtcg
    26821 aagcgaatca cttcgtcgat caacgggtga ccttcgagca gcgcggcgaa gctgtcgccc
    26881 accagccagg caatgtgggc tgtcggccag gttttgcgca gggcagccag gaccggcagg
    26941 ccgtggacga tgtcgcccag cgaactgggt ttgatgatca gaatgcgctg gtatggtgtt
    27001 agcattgggc tcattgaaag tcgcgcatct ggcattcagg tccgcgctgc ttgcactgct
    27061 ctcactgctg cgctgctttc agctgcactg cttgaagctg cgctttcaag cagtggcaca
    27121 cacaccgctt tctagcggtg gtacacgcgg aggcgcgtca cacggaggcg cgtcaatctc
    27181 gcgcgtcgga cgatagcgcc ggcgtcgcgt gccgcgccgg ggccgggtgc tcatcagggc
    27241 tgcgccgtcg ccggcggcgt tccggccagg ttcgcagtgc ctccatatac gtatagaaga
    27301 ccggcgtggc aaatagggtc agcatctgcg agaacagcag gccgcccagc accgccaggc
    27361 caaggggacg gcgcgcctcg gcgccggccc cgacgcccag cgcgatgggc aaggcgccca
    27421 tcagggccgc catggtcgtc atcatgatcg ggcggaagcg gatcaccgag gcctgataga
    27481 tcgcctccag cggcgtatgc ccggcgcgct gcgcctcgat cgcgaagtca atcatcatga
    27541 tgccgttctt ctttaccagg ccgatcagca tgatgatccc cacaaaggcg tacaggctca
    27601 actcgacatc gaagaccatc agcgttaaga gcgcgccgaa gcccgcaaac ggcagggccg
    27661 aaagaatagt aatcgggtgg atgaaactct cgtacaggat gccgaggact atgtagatga
    27721 ccagcaccgc cactatcagc agcagtccca ggttctttaa cgaggattgg aagacctggg
    27781 cggtgccctg gaaactggtg ctgatgcccg ccggcaacag ttcgcgcgcc agtgcgtcca
    27841 cctgtgcggt ggcgctgccc agggcgaagc cgggcttgag gttgaaggaa atcgtgacgg
    27901 acggcaactg gcccgagtgg ttcaccgaaa gcgggccgac ggcggggacg agtttggcca
    27961 ccgcctggag cggcaccagc tttccatggc gtgtgcgcag atagagcagc tccagggccg
    28021 ccggatcgcg ctgatactcg tcggccagct caataatcac ctggtactgg tcctgcgggg
    28081 cgtaaatggt cgagatttgc cgggcggcat aggcgctgga cagggccaac tcgatctgct
    28141 cggccgtgat gcccagcgtg gaggctttgt cgcgatcgat ttcgactatg acctgcgggt
    28201 tcttaagctg gacgtcagta gtcacgtcca caaacccgtc cagctcactg atgcgttggg
    28261 ccagcttcat tgcccccgcg tacatctctt ccatgtccgg gctttgcagc gtgaactgat
    28321 actgactctt tgacatacgc ccgccgacct gaatcggcgg cacgttctgc agatacgcgc
    28381 gaatgcccgg cacctgggcc agcttgggcc gcagttgctc gattatttca tcggccgaga
    28441 gcttgcgctc atggcggggc ttgaggctca ggaacatgcg tccggcattg gccccgccca
    28501 tataaccgcc gcccacgctg gccatgaagg acgccacgtt ctcgtccgtc ttgacgatgt
    28561 caaccagcgc tagctggtgg cgcaccattg actcgaagga aatgccctcg gccgcctcag
    28621 tgctgaccat gatgcggccg gcatcctcac tgggcagaaa gcccttggga atcttcacga
    28681 acaggtaagc agtgggtatg agcacggcga ccgaaagcaa catcgttgcc agccggtgcc
    28741 gcagtacgcc gcgcaggccc cactcatacg cggccagcat gccatcgaag aaacgctcag
    28801 aaagcgcgta aaggcgtccg tgcccctgtt tggcatgcgg gcgcaaaagc cggctgcaca
    28861 tcatcggggt caacgtcagc gagaccacac cggagaccag aatggccgca ctgatcgtga
    28921 tggcaaattc atgcagcagc cgtccgacca ggccgcccat gaacagcacg gggatgaaca
    28981 cggccaccag agagaccgtc atggacacga tcgtaaagcc gatttcgcgg gccccgttgt
    29041 aggcggcctc caggacgccc tcgccctgct cgatgtgccg tacgatgttc tcgagcatca
    29101 cgatcgcgtc gtcgaccacg aagcccaccg aaagcgtcag cgccatcagc gagaggttgt
    29161 ccaggttgaa ctcgagcagg tacatgacgg cgaaggcgcc gacgatcgac agcggcatgg
    29221 cgacgctggg aatgatggtg gcccgcaggt tgcgcaggaa cagaaaaatg accatcacta
    29281 ccaggcacag cgtcagccac agcgtgaact tcacgtcttc gaccgactcg cgaatggaaa
    29341 tagagcggtc atagagttcg ctgatggtta cgccggccgg caactgctgg cgaaaggaag
    29401 gcagcagggc cttgacgtca tccaccacct gcacggtgtt tgttcccggc tggcgctgca
    29461 ccgccagcac cataccccgc acgccattga accaggcggc gcgcttgtcg tcttcgacgc
    29521 tgtcgatcac ccgcccgagc atctccaggc gaatggggtt cccgttgcga taagccacga
    29581 tcagcgggcg ataagcggcg gcgtccatga gctgcccggt ggattcgagc gttacggccg
    29641 tctcggagcc gtagagcgtg ccgaccggga gtttcacgtt tccccggctg attgcgtcgg
    29701 ccacctcgtc gatcccgagt tcgcgggcgg ccagctcacg cgggtcgagc tgaatgcgca
    29761 cagcgtaatt ctgggagccg aagacgttca cctgcgctac gccgttgacc atcgagatgc
    29821 gctgggccat gagcgtctcg gcgtattcgt tcagctcgta cagcggcagc cccggggcgg
    29881 tgacggccag aaacaggatg ggctgatcgg ccggattcac cttgcggtag gagggtgggt
    29941 tgggcatttc cggtggcagg cgccgttggg cgcgggcgat ggccgcctgc acgtcctgcg
    30001 ccgcggcatc gatatcgcgg ctgaggtcaa aggtgatggt gatgctcgtg ctccccagcg
    30061 tgctgttgga gctcatgctc tcgatgccgg cgatggtcga aaactcgcgt tcgaggggcg
    30121 tcgcaaccga ggaggccatg gtctgcggac tggccccggg cagattggcc gtaacgttga
    30181 tcgtggggta atcgacgttg ggcagatcgc tcacgggcag cagacggtag cccatgatcc
    30241 cgaagatcag gatagccgcc atcagcagcg tagtagtgac ggggcgccga atgaaaagag
    30301 cggagatgtt catgggtgcg tgtccggccg gctcgccggg gcgggctgcc ccacgtctgc
    30361 cggctcccgc acgaccacgc gcgtgccgtg cgtgacccgc aggtgtccgt cggtgacgac
    30421 acgctcgccg gcgcgcaggc cggtttcgat gatcgtctct tcacctacgg cgaagccggg
    30481 tacgaccggg cggctttccg cgaccaagtc ttcgcttatc acgaaaacgt actggcctgt
    30541 ttgcgaggtc tgcacggcgg cggacggcac caccaccgcg ccgcggcgcg tcccgagcgt
    30601 gagcgtgacg ttcacaaatt gcccgggcca gagccagtgg ttggcgttac cgaatgtccc
    30661 cttgagcgaa atcatgccgg tggcgctgtc aaccgcgttg tcgatgaagc tcagcacccc
    30721 gacctcgttt tcggcctgtt cttcgcccgg aatctccacg cgtacctgca gatcgccggt
    30781 gctctgagcg cgcttaatgc gacctagctc gcgctccggc agcgagaagc tgacgtagat
    30841 cggcgaaagt tggttcagca cgaccagggc cgtctcgttg gcactgacga cgttgccggg
    30901 atgggccaga atctccccgg cgcggccatc tcccggcgcg cggatcgagc agtattccaa
    30961 atccaggcgg gctttggcga gcgcggcgcg atcggcggcg accgcggctt tgagtgattc
    31021 ggccagtgcc cgtttgttct gcgattcgcg ctgcgtgccg aggccgcgct cctgcaaatt
    31081 ctcttgccac tcggcctcga tgagggcgtc gttcataagg gcgatgtttt tttccagcgt
    31141 ggcctgggcc tgctccaggg cggtctgaaa cgggcgcgag tcaatcgtga acagcaagtc
    31201 gccggctttg acgtcctggc catggacgaa gtgcgtttgc gccagttgcc cgctgacctg
    31261 aggcttaacg gtgacggtcg tgaacggctg aacgcgccca atggcatgca gctcgagcgg
    31321 catgtccttc tcgaccgcgg ccgcgatagt taccggggcc ggaggctgcc gtgcgggtcc
    31381 ggcggccccg gccgggggct gcagccggtc gcatccggat agtcctatca gggtggccag
    31441 cagcagcggg atcggcccag ccgctcgggc accgaggcat tgccggctga tcaaaaattc
    31501 ctccataccc gcagttctgg tgagggttgc cgttggctcg ccagttcctc gaaactcggg
    31561 tgggcacgtc cctggtccct gcggagttta tgggaaaatt ttacgagccg gacatacgcg
    31621 cagcaacgtc gcgaattgtg gcgccgccat cgcggggggc tcgccagtgc aagaggtgcc
    31681 gcagtgcctc ctgaaccggc tcaaaacgac cgatagcatc cattgtgcag agctgttgcg
    31741 cgacagccgc cacggccacc aaggacctga aaatggcatc gggttttaag ccacatactt
    31801 ggggcgcccg cccgaccgcg agtgcaaacc tctgctacgt ggccggacag accgaagttg
    31861 cccgcagccg cacgccagac cgcgagcgcc gggtccccca tcgcgcaccg tgtcgcctgc
    31921 aatggtggga cagcgattcg aagcggaccg caagctgcgt cggcgagacg accaatctgt
    31981 ccgagcgcgg tctcgcggtg cgcgtccgcc atttcctgtt gaccgggacg cgcgtcgaaa
    32041 ttctgctggc aaacctggct ggcgacccga ggcggatcag cggcgtcgtg gttcgctgcc
    32101 agcgaatcct cgcggacact ttcgaacttg gcatccggtt cgtggattaa taacccggtc
    32161 aaaatctcgc gccgcgttaa attaatcgct ggtccggttc tctcgcgcat cagttgatcc
    32221 gcgatcactc gctctcgcga aacggaagat ttcgccggta aagagccaac tgctcctcga
    32281 tccaccgcgc ttcgtgtacg cggccggcct gacgggccat ttgcagaacc tgctccgcgg
    32341 tcgttacggc ctcgtcgaag cgcccgcact cggcgtaagc tgccgccaga gcttgcagca
    32401 gggtcgggtt tgggccagcg gcttcaattg cctgctgcac gatctgcagg gcgcgcggcc
    32461 cgtcgcgcag cgtcacgtcc ggggaggtgg ccagttgcca cgccagcggt cccgccaccg
    32521 agacgttgtg gggctcgtgg tgccaagctt ccgccaaaac cgccaaagcc tcgcgcgcgt
    32581 gtccgccaag ctgcaaggcg tgcgctaggc tgcgcagggc cgcaacgttt gccggctctc
    32641 gcgctaggag ctgccggtag cgcgaagcgg cctcgtcata gtgacgcagc tcaagcagcg
    32701 cgccgccgac gctcaacaag gccgccgtat cgttcgggtt gtgccgcagc gcttgctcga
    32761 attgcgttat cgcggcggca tagtcacgac gcgccgcgag aatacgcccc agccggtggt
    32821 gcaggtcggc aaggtattca ggccggccgg cagtgcgccc cagaagttct agcgcctggc
    32881 ggtacgcatc cgccgcctcc tcagggcgcc ccacgttctc gaggcacgta cccaggtagt
    32941 agaagttgtc cgggtcgttt ccggaaagct gcgccgcctg cgaaagttct tcgcagccct
    33001 cctctgtcag gcctaattcg atgaaacacg ctcccagcga gcgccgcacg ctccagtcgt
    33061 ccggttgaag ctcggccgcc cgttgcagat gccggagggc ctgctcgcgc agcgccgcat
    33121 cctccttcgc cgtcttggca tagaactgcc cgagctgaag atgtgccgca ctttcctcct
    33181 cgtgttcaaa cgtggagcga tcgggcgggt tcgtgccgat gaaacccgcg gccagcgcgc
    33241 caagcccggc atagaccgcc agcggcttga agcgccgcgc tgccaaaagc tgaacgcccc
    33301 gcaccaggcc cacagccgcc agcaggatca acaccgggat aaccggcaga cgaaaacgcg
    33361 ccggacagaa gaacaggacg atgccggccg catagctcgg cagaaacagc aggggcagca
    33421 gccaggtgcg ccagttcttg cccagcagcg tcaggccggc gatacccagc acgccgacca
    33481 gcgggaaccc aatccaaaaa aaccgtgcga ctggcgcgag gccggcaaag tactcgatcg
    33541 ggtgatcgtt cgggagttcc aggggactga agaacaagcg ccatttgcgc cacatcaagc
    33601 gcacccagtc tgccggattg gaacgaatcc acgtcgtggc gcgctcaaac cagaaatcgg
    33661 aaacctccgc gtgcgtgagc ttgcggccgg ccttggcctc cggcagcgct tgcgttcctt
    33721 caaagcggcc cgaccagaag tagccgatct ccggaacaat ggcggagcgg ccgtcggagc
    33781 gcggattgtt tcctatgtag aagttgatgc cgccgctgga cgctatgccg acccattcgc
    33841 cgcccaccac acggtttctc agagtcacgg ccgaaacggg cagggcggcg cccaccagca
    33901 gcaatgcggc ccacaaccag cggcgcggcg tacgccggct ctgactttgt ggccatagcc
    33961 agagcgcgat cagcaatgcc ggcgcgatga tcagaatatt ggcccgggtg atcgcggaca
    34021 gcccccaagc caacccggcc gtcagaaata gcggccagga gcccttttgc gcaccgcgca
    34081 cgaacagcag catcatcagg acgtccagcg atacttccag gctggccgtc tggagctggt
    34141 tatcaaaaaa gatcatgggc cagtaaagcg ccgcgatgag cccggcgagc agcccgactg
    34201 ccggtcccgc caacgccagg gccagccgcg cgatcaggta gcagctaaac gtgcccagca
    34261 gacaccccag caagcgcgcc gccagcggat tggggccgac aatcgcgtac agcgccccga
    34321 gcaggtagta gtagagtggc gcgcgaaaga atggagcggc ccccacgccc tggccggtgg
    34381 cgaccgcctg ggccagctca tgatgagttt gttcgtccag ggtcgggtgg ctgaagaacg
    34441 ggttgttcgc cgattgaccc caccaaaacg ccaaccgtag cccacacgcc gtcagcacga
    34501 tgagcgccag cgcccaggtg tggacacgtt tccagctctc agacggcact gccgtgtccg
    34561 gcgaagaact cggccgggcc cgtcgcgcgc tgaccattcg attctcctgc ggctcgttca
    34621 ggctagtggt aataacacgg gcggagcgcc catgccacat ccctctgcct acgccacagt
    34681 cctaaggcgg gcttcggctg tgccgcttgg tggggctact tcgccagcct ggcttcaatc
    34741 cagtcgcaca gcggatcgag cccttcgccg cgcagcgaac tgacgcccca gatcggacgg
    34801 ctattatcga tcgcgtcgcg gagttggcgg atggtcgtat ccgctcccgg caagtcggcc
    34861 ttgtttacca cgaacccagt ggcgatctcg accacgccgg ccttggcaaa ctgcaccgca
    34921 tcgcccgcgc ccggcatgac cagcagcaga acctcatctg ccaacttgcg gacttccgtg
    34981 tcgttttgac cggctccgac cgtctcgatc agcacgatcg ggtaacccgc cgccgtcagc
    35041 agccgcgaaa tctcttcgca atgcggcccc agcccgccgg tctggccggc cgaggacaag
    35101 ctgcgaacga acagcccttc ccgcggttcg gctgacatca ttcgcagccg gtcgcccagc
    35161 agcgagccgc cggtgatggg gctggtcgga tcgatcgcga tgacgcccac cctgtgttta
    35221 cgctcgagca gcgccccggc cagccgcccg atcagcgtgc tcttgcccac gcccgcggag
    35281 ccggtgatgc cgatgcggac ggttggccaa tcgacggccg gcagatgatt aagttctgcc
    35341 ccgcggccgg ccgcgaccca actcatggcg cgggccaagc cggccggcgt gcttacgtcg
    35401 ggcggatttt tttccgcgtt gcgccgtgcg cggcagcgtc ccgccaactc gcggatgaat
    35461 tcaatgatgg cgctcatcgg cgtgccgggg ttaaacacgc cgtcgatgcc ctgttcgcgc
    35521 agggccttgc cgtcctcttc ggggatgatg ccgcccacga tgagcggcat gtccggcacg
    35581 cccgccaaac cgcacagccg ccgaacctcg ggcatgatgg tcatgtgggc cgccgagagc
    35641 aggctcacgc ccaagacgtc gacatcctct tgaatcgcgg cctgcacggt agaactcggg
    35701 ctttgccaca ggccggtata aatgacgtcc atgcccgcgt cgcgcaagtt gcgcacgatc
    35761 aatttggccc cacggtcgtg cccgtcgagg ccaatcttcg aaatcagtac gcgaatcggc
    35821 cgatccattg cttctccaat gttagaggcc gcgcgccaga ctctgggttg acccaaaatg
    35881 acataacccg gcggccgcgt caaaacccgt actgtatcag gcgcaacgtg gatgacaacc
    35941 gggccggctg cgggcgccgg gcccacgggg cggcatccag actggtgaaa tggtgaacag
    36001 agcggggcgt tgttctcgga cggtcgagat cgcaattcgt agaaaaacac tggcgtgaac
    36061 ccgggccgct gcgctgggca actgcggcgc gtacgccggg ctcggatgag cttgcgtggc
    36121 gcaggggcgg acggggggcg gatcggggtt gacgaccatc tgccggccga ccgacaggcg
    36181 cgcttcaggc cgcccgaaaa gaatgccgat gcgcgataag gcacgcaacg cactccgggc
    36241 tggccgcggt tctcggcagc gccgcgacgg ttgcacggca gcgctgcgta ccgggctggc
    36301 gacagacctg cctgcccgcc cctgcaggag gccgctatga aaagaatctt ggcttacaac
    36361 gtcccatggg tgattcttct gtgcgcctgc aactcgacca gtccgcggct gaacgccccg
    36421 ccgcacggct gtgccgaggc ccgccatgaa atgcaggcca tgtacacgca catgagcgac
    36481 aacgcgctgc tcgcggacat gaacctgtcg gacgtcgatt tcatcccgca tcggcccatg
    36541 ctcagtgcgc ggggcgaaga gcggctgtgc cggatcgtcg ccttgatgca ggagtacggc
    36601 ggcgagattc ggtacaccgc tgaaaacggc gaccgcgatc tggtcgatcg ccgcattgaa
    36661 gtgatcattg acttcctgac cgaagccggc ttgccggccc atgcggttcg agagggtctg
    36721 cctggggggc gcggcatgga ggcgcgcgag gtgttgctga tcaagctggt ggaagggacg
    36781 tattcgcccg agcggtccgg cgggggagaa aagtcggggc cggggcagta cgaaaagtaa
    36841 aaggagagca cccttgttga agtattttgt gccgccgtca ctggtgctgg gggcagtgct
    36901 gctgtggtcc accggctgcc agtcagagcc gaagagcgcg acgccgctgc gtccggccgt
    36961 ggaagattcc gggccgcgac tgaacgcttc aacgtacttt gcgcacgggc atttgctgga
    37021 gcggcagggg aattacgagg gggcggtcga ccaataccag cgcgctttgg ccctggcgcc
    37081 gcagatgacg tcagcccgca atcgtttggg aatcacactg aacaagttgg ggcggcacgc
    37141 ggaagcgacc gctcaattcc ggcaggcggt tcagcagaac ccgacggacg ggtatctgca
    37201 aaacaacctg ggtttcagcc tgtaccttga aggccggtac accgaggccc tggcggccct
    37261 ggccgccgcc cttgaactgc agcccgacta cgggcgcgcc catatgaaca agggcatagt
    37321 actggctaag accggcgact acgccggcgc gctgacggag tttacactcg ccgccggcga
    37381 agccgaggca cactacaatt tggccgtaat tcaggcggaa gcgggggact acgcggccgc
    37441 ggcgcgctcg ctcgaaagcg cgcttaacgt caacccgagc ttcgaggcgg cccggcagca
    37501 gcttcacgtc gtcgcgcggc tggcggcggg ggccgggacc gcggtggcgg cggccccagt
    37561 tgccgagaac gcgttggcgg aggccccgac tgtagaaact ccggcggctg aatctccggc
    37621 cgcagagacc cccgtggcag aggctccggc caccgcagta gacacgagcg cgccacccgg
    37681 cggtgcacca gcccaagatc tcgcggccga cgggatcacg ccggttcgcc cggaggctgc
    37741 ttcacccgag cttttggcga aaatggtcga gcattggccg cttgagacag caaagcaaga
    37801 cgggggcgaa ctgcacacgg accgtgcgag cgctcgagaa aggcagccgg tcgtgtccgc
    37861 ggccagcgcg gtgttggtca ggcgccccgc gaccgtgacg caataaggct caggctcccg
    37921 cgaaggcgct cataagggcc tggccggtct tgtgcagcgt ctcgccggcc acaatgccgg
    37981 cgcagatggc gaccaggaaa cgcttggtcc cacttggcgc cgcgatggat aacatcagcg
    38041 ccgtcagccc gcccacgaac agcgctatcg aataacccgc cggaatcatg aatgccaggc
    38101 caatgctggc agcgctgggg acgaagcgcg cggcccgccg cggcagtccc ttctcgagta
    38161 ccgacaggat cagtccggcc ccggccgcga taatcatcgc caagccggtg cccggcggga
    38221 tcaggctgaa tccatgttgg aacagttcgg ccacggcttt cagcggcgcg actgccggca
    38281 agttccagcc ctgtccgaag ttctcccgtg gcataaacat caggtagacg gcggagccgg
    38341 ccagcgatcc cgccaagacg ccacaaatct gagccagcgt ctgcaatcgt gccggagtac
    38401 ccaccaggta tccgcatttg agatcgtcca tgaggtcggc cgattggctg gccgccccgg
    38461 ccgcgacgtt tgaagccatc aggttcgcca ccgcgttgtg cggggccatc gctccgacag
    38521 tcaactgcgc caccttgccc atctggccca ccggcgtgta gccggcctcg cccgaaaccc
    38581 gtgtggcgat gattgccagt acaaaagcca gcaatacgcc gcctacggcc agccaccagg
    38641 caatgccgaa caagctgatt tggagcgcca cggacgcggc cagcacgaag atcagaccag
    38701 ccgcggccca cgaccagggc acgtcaccac tggtgggcgc tgcggcagac gattggcggt
    38761 ttcggctgcg gctgcgccat gcggtgcgga cggacaagct gaacgacgcc agcgaggcca
    38821 ccaccatgat tgtcgcgcct ggccacacta accaactcac gagatcgctg aacgtggtgg
    38881 ctgtcagtgt ggcgttctgc ggctgcgatc gcatgtacag cttggcgatg acttcgcgcc
    38941 agtcggcgtt ggcgctggcg gcaattaacg cgtcgcgctg cgcggcggac atgaggccgc
    39001 gccattccaa gcgcccggcc gccggctcat agcgggcctg ttcgggcgca agcttctgca
    39061 gatcaacgcc cgccggcaga gtcgacactt tttcgacgac cgtcaggcgg atgctgccgt
    39121 tcttgatcag ccgcgggacc atgacgccat aggccaaaac cgccccgatc aacagtgagg
    39181 cgccggcccg gaagccgatc agcccgccga cgccgaacca cagcagactg ggctgcacca
    39241 tgaaggtcag caccgtgggc ttgattccct gcaccgcacc gggcagcttg aatggcgcga
    39301 tcttccagaa cgaagcagcg aaggacatgc ccgccgcggc ggccgccccg caaagcagcg
    39361 cggcgaagcg ggtcagcgct tcgcggccgc gcgtgtgcat ttcgcggagc atttccgcgc
    39421 aggcaacgcc catggggaac gtcagattgt cgtgcaatag gagctgccgg cgcagcacga
    39481 ctgcgatcgt gatgcccacc aggcacaccg agagaatcca gagcgacaac gtgtgccatg
    39541 gcaaataaac gccggtcaga atggtcagcg cgggcaccgc tgcgaccagt cccgccgagc
    39601 cgaccagcgc cgcggccgaa caagcggtct ggctgatgtt gttctccagc ataccccagg
    39661 ggcggacgcg cccgcgcgtc agccaactca ggcctgacca gaaggcatag gccagcagaa
    39721 tggcgacgat ggccatgttg atgccgatgc cgagctgcaa gccgagatag atgttacagg
    39781 ccgacaggac ggcgcccatg agcatcccgg ttaacacagc tcggggcgtg aactgagcag
    39841 aggccggcgt tggctgagcg ggttccaagg ctgtggggac ggaggcgggg acgaaccggg
    39901 aaggttgcat tccggcaggg tgcacggact cggaagttgg gccggagctt tggctgggcg
    39961 tgggggcaaa gctggggctt tgcgctccgt cttgcgacag tggttgatgg gacggtgctg
    40021 tcacgggcgg cgggctccga accaatccca gcccatacga ctgcgagcgg atagtagggc
    40081 gtaaaggggc ggcgcgtcaa tcgccggctg accgtcaccc atgacggcac aaagctcaac
    40141 gtcttcgaga tcgatccatt cgtgttggcg ctgactgacg ccacagctta ttgacggagg
    40201 gctagacgag aattgggagg ccgtcctgaa cgacctagta cttcggttgg aacgcgtcgg
    40261 cgaagtgctc gatctcgggc tcgccttcgt ccggcagcac gatgccgact acgtcaaagc
    40321 ggcacgggcg gtcggtccag ttccgctcgc gcagaaacca ttcggcgcag cgccgcatct
    40381 tgcgctgctt gaccgcgcct actgactcct gcggctcggc gaattgacgg ctgcggcgcg
    40441 tcttcacctc tacgaagacg accgtgtcgc cgtcacgcac gatcaagtcg atttcaccca
    40501 ccggggtgac gtagccgcgg gcgactagct taagcccgcg gcgtttcagg taggtttcgg
    40561 ccaggcgttc gcctttgtcg ccgtattcgc gagtcgagtg cggagcatgg cccggaacgg
    40621 tggctttctc cggaaggcat gcccgcgctt ggcgcttggg catggcaccc aactcctact
    40681 ggcaacggaa caactgacaa aactaacaat taacaactaa taattaacga ttgccaaccg
    40741 cccgcggttg ccacgcgcgc ctaggcctcg ctggtcgcca tttccgggct gccggtcttg
    40801 gctgcggcct cacgctcgcg ggccgcctgg gcgacttcat ccgcctcgcg caaagcgcgg
    40861 gccttggcca actgggcctc caacgccgcc ttgcgggctt ccgccgaaat gcggcgttca
    40921 cgcagcttgc gtgcccggcc gacgcgttcc cgcaggtagt acagcttgca gcggcgaatc
    40981 ttgccgcgtc gcacggtctt gatccgcacg accttcggcg agtgcagcgg gaaggtgcgc
    41041 tccacgccct cgttgcccac cagccgccgc accgtgaaca tctcgtccag gccggagccc
    41101 ttgcgtgcga tgaccacgcc ttcaaaatcc tgcaggcgct ccttgttgcc ttcgacgatc
    41161 ttgatggtca cgcgcaccgt ttccccgatg ccgaagtcca actcgttctc acgccggtat
    41221 ttcttctcga cggcgtcaat cagtgggtgt ttcataagga gcgcctctct ttctagttat
    41281 gcgctgcgtt tggcgattgc gctcggcccg gcttgcgcca tcggacacgc gcaataatca
    41341 atgcagttca aagccaatgt actttctatc gaccggtctc cattgcagtg agacccgtcg
    41401 ttcccggacc cgtttttatc ggggctgtct tgaaaatcag tcccgcattt cccttccagc
    41461 aggtccggtc gccgctgccg cgtgcgttct tccgcttggg cccggcgcca ggcgtccacc
    41521 tgggcgtggt tgccggatag caaaatctcc ggcacgctca agccgccgaa aacacgcggc
    41581 cgggtgtatt gcgggtaatc cagtaagttg ttttggaacg attccaagcc cgccgcttcc
    41641 tcatgtccca gcgcgcccgg cagcagccga acgacggcgt cgacgaccgc caaggccgcc
    41701 acttcgcccc cggtcagcac gaagtcgccc aggctgacct cttcgggctg gagcaactcg
    41761 atgatgcgct catcaaagcc ctcgtaatgc ccgcagatca ggagcagccg ctcggccgcc
    41821 gcgtaacgtg ccgcgccggc ctggttaaac acgcggccct gcggggtcag gaggagccgc
    41881 agggccgggc gaggatcgag tttctcgatc gcgtatacgg cgtcgataac gggctggcac
    41941 atgagcacca tgccgggccc gccgccgaac gggcgatcgt ctactttctt gtgcgggtcg
    42001 gtcgagtaat cgcgaagctg gtgggtgtga atctcaacga tgccggcttg ttgcgcgcgc
    42061 cccagaatgc tggccgaaag atacggctgc atgagttccg ggaataatgt caggacgtcg
    42121 atacgcatga cgcgacgcaa ccattggctg atcggttttc gttcgcagtg cccctacttc
    42181 gtaacaccgt gtcgcttgag caggttagcg acggtttcgg tcggctgagc gccctggctg
    42241 agccaatgct tgatgcggtc cgtatccagc cggacctgca tatcggtgtt gcggttggcc
    42301 ggatcgtagt ggccaagctc ttcgagcacg cgcccgtcgc gctgattgcg cgactccatc
    42361 gcggccaagc gaaacaccga gcgatgcgaa cgcccgaatc ttttaagacg caacttgacg
    42421 gccatgtacc tctccggcca tgttttggcc cggggataag cgttagcggg gcggcctgtt
    42481 cgccggccct gaccgcgatt gcaccgagca acgcggagcc cctgagtata atggctcgta
    42541 aagggcgtgc caaggggcaa aatgg
//
LOCUS       DAOSYS010000004        38044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_867_length_38044_cov_5.446076, whole genome shotgun sequence.
ACCESSION   DAOSYS010000004 DAOSYS010000000
VERSION     DAOSYS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 38044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38044
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_38044_cov_5.446076"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLU99_00535"
     CDS             complement(<1..1182)
                     /locus_tag="PLU99_00535"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO15567.1"
                     /translation="MCIRKPWSALALGASLIMLLSLCGCPPEPPPPPNQSPVANAGPD
                     QTVSAGSTVTLNGSGSSDADGDPLTFQWTQISGTTVTLDNADTDTASFTAPNVSEVLT
                     FQLTVDDGNGGTASATTSVTVTTTPLTKPILFIANVSGDSVTAYDITTPSAINGNIPP
                     NANLAGAQTLLSVPDDLLIDNAGNLLVLNFGSNSITSYPNALTLGQINGSVAPTRNVQ
                     GAATLLNRPASMTLNRAADLLFVSDEIGGNIHVFAGASTAAFNGNLAPVRNITSTDMN
                     GPFGIRFGANDELYVANFDANTVAVFANASTLNGVVNATRILNSPVFSKPYGVFVDAN
                     DTLYVVNSGDGGALANRVNVFFNAATLNGSVMPDSTLIVPGATDLTVIAVDSAGTGYI
                     AD"
     gene            complement(1390..2697)
                     /locus_tag="PLU99_00540"
     CDS             complement(1390..2697)
                     /locus_tag="PLU99_00540"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO15568.1"
                     /translation="MFQRQQWSLFALGLSTLALICLTGCPPEPPNQLPVANAGADQTV
                     QAGTNVTLNGSGSTDPDGDPLAFQWTQTGGDAVVLSNGTAANATFLAPNAATTLTFEL
                     RVSDNLGSDTDTTTVTVTTTPPPANTPKLYVANFAFNSVTAYNVSNPNAVNGNIAPTA
                     NLAGAQTLLTLPIDLVPDANGALLVLSTAAPKITSYGSAQNLALINGNVAPARNVQGA
                     ATQLVNPVSLALSRTNDLLFVADAVGGGAIRVFAGASTNAFNGNLAPVRNILSIDLVN
                     PRGINFGANDELYVANLGNSTVAVFANASNLNGQVRATRVLQSALFAGIVDVFIDAND
                     TLYTVSGPAGGNRVNVFANAATLNGPVTPDVTLTVLGAVQLNGVTVDAAGNGYISDPN
                     AGPSGAIYGYDNIATRNGTIAPDRTLTGENTLLGGPAAVFLSQ"
     gene            complement(2959..5241)
                     /locus_tag="PLU99_00545"
     CDS             complement(2959..5241)
                     /locus_tag="PLU99_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15569.1"
                     /translation="MSPAKHAPIAAGMPERATQCARCQRPIESGAPGRLLCSSCESLQ
                     ATQAPAAAAWFHRAWLPVVVGCACFLNSLPNGFVYDDVPQVAENPAIRSITNFHAIWL
                     SGWTNTYSGAEARMHPNPDLLYRPFTVFTLALGHSLHGLWAPGFRLVNIALHGLTCFL
                     IWEFLRRLFEDRSLATWGALLFAVHPVHVEAVAYIVGRAEVLATIFSLLGLLVLVPKN
                     RPSDTGRAWRAMPLFFLALLSKETAVSYIPMALIVLHACRGRTPIRGVRWWGVHTLCL
                     LLPLLVYLRLRVWALEGAFMRAPAALLNPIVDAEMPARLLMPFEVLGHYVRLLLFPLH
                     LSADYGYEVFNPRAGVNAMTLLGLAAAAGLVAALLGYLRAGGHWRRLAVCAALFIAAY
                     ALFSNSLLLIGVSVAERLMYWPSVAACGLLAALALSLGRRPAGAARANLAAPRFATVA
                     GTLVLAAFATRTVIRNTDWYSALRLATHDAACYPQSAALNAGAALQYIDVALQTDDPA
                     ARRQNLERADEYSARALQHYPVHWGNHRMRSDILSLLGQADMALHHIRQAVQLNPGET
                     LLRRRLGRRLFQAGQFAEALPYLEEEAALAPDDYVVLTMLGEAHLKLGRSREALPHLK
                     NALRIAPDDRAPLLLMSEALLKLNELEQAVPVLEKLAAVAPHEVRPPAVLAKLLVQRD
                     PPAALRYAQQAHALLPQDPETALLLADALIATGRSDEGLSLYVQTLDRLSPEHPLRRP
                     VESRLETLRAATTQPSADRP"
     gene            5666..6559
                     /locus_tag="PLU99_00550"
     CDS             5666..6559
                     /locus_tag="PLU99_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO15570.1"
                     /translation="MPSKLDTALGEYLKQIKNYPLLTAEEERELGAKIRALAEAQEHQ
                     AEKNIPPETMERLRQEAAEARERLAQANLRLVISVAKNFRNRGLPMEDLVNEGNVGLM
                     NAIDRFDPAVGSRFSTYAGYWIDQSIRRAVQNASQMIHIPSYLMEQIGQMKLAMRELE
                     EKLGRQPSMNELAKHMDITPRKAAAISQAIRACTTRTQDSLAEGETSLTEALPDTRTP
                     QPYQAVFSQSDSDFVTRVLSRITEREALVLKLRYGLNRRDGKRMTLKEIGEAVGLTRE
                     RVRQIEKDAARKLQEYVNEYY"
     gene            6833..7138
                     /locus_tag="PLU99_00555"
     CDS             6833..7138
                     /locus_tag="PLU99_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15571.1"
                     /translation="MLTMRRTSLAAGTRVKVKTPGPVPHWSVWEPCQRTSSHVKKRLQ
                     DMFFKGDRRITAEVVYIASESDREKLKAAGRVKLYLRDAAGSSVTILADANNLAAAS"
     gene            8176..8865
                     /locus_tag="PLU99_00560"
     CDS             8176..8865
                     /locus_tag="PLU99_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO15572.1"
                     /translation="MKRIVAVVAAGLCACLVQAELFWISYDASCGLYPEEVGWTRTTH
                     GGGAVRSLADGILTLDSTADSMICDGYGIYRPTTPGPGETFVCEWRMCLAYHSGFGES
                     LLSMQADDGGGVRFRYWMDHLQSEEGWIVPITAGVFHSYRLESTDMYTYSLWLDGVYM
                     GSDAFDRPGPPVPYAAFGDCTYGTTPQSSLTQWEYFRFGVVPEPSSLALVLALTTCAC
                     VARRRRARWES"
     gene            8879..9382
                     /locus_tag="PLU99_00565"
     CDS             8879..9382
                     /locus_tag="PLU99_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15573.1"
                     /translation="MLSKVSVLMSCALCALPLAQANQVRVWHATGSYNIDPDFHTVTI
                     TSPGTFKIQATDGSGGLGYIAKIDVAFAGLPGQVIVYVVRDPSEVGGAQHEPGAAEVW
                     AIDLSACETAGTTGTVAELRTTGNYGHVTDGDTLYASDVGAVGGGSRRWMSASQGLSR
                     CSRHESF"
     gene            complement(9440..11743)
                     /locus_tag="PLU99_00570"
     CDS             complement(9440..11743)
                     /locus_tag="PLU99_00570"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO15574.1"
                     /translation="MGEPSEPSLESSPGARPDPDLTLAHLDRLPALSSAAVRLLAMAA
                     EPRASVADAVEILRADQSLTAKVLSVANSAWGRLPGPVDTLEQTIPHLGFAGLRSIAL
                     TASVFDCLPIRPAGDDATFDPPEFWKHSLAVGCAARALAEALRPLSVDPQEVFVAGVL
                     HDLGKVALSAVFPKTYQRIAAEANRACGDIADFERELLGTDHTVAGRRLAERWHLPRL
                     LRDVIWLHHLAGDALPASVANRQLLALVLLADTLARERRIGYSGNHVFYEHSPRLAER
                     LGLSEGQLSETATRLNQEVAELTKELGLEHKANGGVHSLGLSRTDAEQGRLNTELTLA
                     NERLAAGARYFRALGQFDRLLGAWSDLPKAVAAIAAVAGIVLQRPAVAAFGLRERAPA
                     VDVCWIDARQNTTSVAFADLPAEVRDWLNSPDDVIDSLVTRAPTAIRTLMAPGLHEIS
                     GTGTWLIPIVYQARLAGGIVYGSETDERARLAGEAEELRSFTASLGLALCRANAQAAA
                     RRLADDLAETNRNLQQAQAELLRSRTLSMIAEMAAGAGHELNSPLTVISGRAQMLAAQ
                     LNDPEARRCLETIRSKAHECSRVVSELMDFARPRPPTLAPVNVPELLAELRQVWLTRW
                     KLSGAKLLLSIDQRGPCAAASPSSALNIQADREQLRVLFDELVSNAQHAIRDSEGAIQ
                     IVCQPAAGAAGVEVIVRDDGCGMSPRVLQRAFDPFFSHRAAGRGRGLGLPRAYRIVES
                     HRGHIWLESQPGEGTTAHVFLPAAPPA"
     gene            complement(11709..12332)
                     /locus_tag="PLU99_00575"
     CDS             complement(11709..12332)
                     /locus_tag="PLU99_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO15575.1"
                     /translation="MAINNRDKTGYLKTVFTTGEVAEICSLSQQTVIRCFDSGRLKGF
                     RIPGSRFRRIPRDALIQFMKENNIPLDRLEMGRTRVLVVDDDPAIVEMLVELLERDGR
                     FIVQTAATGFDAGLRTREFQPDVIVLDYLLPDIKGNAVCRSIRSDESLKDVKIIFVSG
                     AIDPGDVDKLFEAGADDFLQKPFSIEQLVNRITELVSGRAKRTKSGV"
     gene            complement(12613..14481)
                     /gene="mnmG"
                     /locus_tag="PLU99_00580"
     CDS             complement(12613..14481)
                     /gene="mnmG"
                     /locus_tag="PLU99_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOO15576.1"
                     /translation="MRRSGYDVIVVGGGHAGAEAAWSAARLGARTVLITFSLEAIGRP
                     SCNPAVGGIGKGQIVREVDALGGLMGRVADETGIQFRMLNRRKGPAVWAPRAQIDSGT
                     YPAVLRRYLENCPNLKIIEASVEAVESEPLGPSCANALPLATSRVSGVRLSDGRVLAA
                     HAVVIASGTFLRGLMHTGEAKAPGGRVGEPAATGLSASLAALGMELGRLKTGTPPRIA
                     RDSVDWSQLAVQHGDERPVPFSFLTDSLPQEQVCCWLTATNERVHELIRANLHRAPMY
                     TGQIQSRGPRYCPSIEDKVVRFADKSAHQVFLEPEGRDSERIYANGISTSLPQDVQAQ
                     IVTSIRGLEHARVLQWGYAVEYDFVPPEQIDASLMTKRVRGLFLAGQINGSSGYEEAA
                     GQGIIAGINAARFCAGASPVIIGRDQAYIGVMIDDLVTRGVTEPYRMFTSRAEHRLHL
                     RYDNADVRLTPLGREIGLVDDERWQKFQAKSDKLAALLQSWERLRFEGRTLADWIRRP
                     EEDGLRFAAVFPELEPYTRELDTWGRALVEIKYAGYLQRQQRSIDEFRRREEQVIPPD
                     LNYSQVPHLRREAVERWSAVRPQNIGQAARVSGIHPTDLSVLLVYIASGQRRASCG"
     gene            14565..15707
                     /locus_tag="PLU99_00585"
     CDS             14565..15707
                     /locus_tag="PLU99_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO15577.1"
                     /translation="MTKTAVSPRSWAVARQVALGLGFSAVVVLLLLWLAGTFHRKIGP
                     GGPEPASRPVRLVKVDQLVPARLASLPVTEPAVGSIRAVHQIAVASKLLAKVTAVNVQ
                     AGQTVTAGEVLVQLDDEDLRAKLRQAAASVAVAETALSQAQVEFERIDGLYKQGNETR
                     IEWERSESALKTRTAELEEARQRQRQAETMLSYATIVSPIDGKVIDKHIEVGDTASPG
                     QVLVTLFDPTRMQLVARVRESLTHRLAVGQDIDVYVDAIGKTCTGSISEIVPEAESTS
                     RTFSVKVTGPCPVGVYSGMFGRLLIPLDEQEVLLIPRAAVSRIGQLDLVEVALQDQSL
                     TEREVVLKGRPVERRAVQLGRTYGDEVEVLSGLRAGELVALQGSAQ"
     gene            15704..19207
                     /locus_tag="PLU99_00590"
     CDS             15704..19207
                     /locus_tag="PLU99_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO15578.1"
                     /translation="MTIDKSPPPEVQHGFTNAIVRTFLNSNLSLVLIFIAIALGAAAL
                     ILTPREEDPQIVVPMADVYVAFPGHSAAEVEQLVSTPLEKLLYQIDGVEHVYSMSRPD
                     EAIITVRFYVGQDRERSLVKLYKRLDEHLDIVPPGVTGWVVKPVEIDDVPIVTLTLTS
                     ASADYYALRRVGEEVAQRLAAVPDTSRSYVIGGQPRAALVYLDPERLQAYNLAPLPVQ
                     RALETANVTQTSGEFARGDHLFRVEAGRIFQRPAELNDLVVGVYGDRPVYLKDVASVQ
                     DGPDETTSYVRFGWGAARGFEQHAGAPGAVVGASASSPGSAGAGEPQPAVTIALAKKK
                     GTNAVWVADAILKAADQLKAGVIPDNMELIVTRNYGQTANHKVNELVEALAIAIIIVI
                     ALLTLSLGWREALIVAVAVPVVFGLTLAVNMLAGYTINRVTLFALILSLGLLVDDPIV
                     DVENIARHFGLRRRASRPIVLEAVAEIRPPLITATLAVIVSFLPMLFITGMMGPYMRP
                     MALNVPVAMLMSMVVAFTITPWLTFHALKRRYWGGDQRINQSRDQEARPQNEEIHDLD
                     ALRQTRLYRIFYPLMAPLLKSKKAAWGFLGAMALLMLAAVALAATRSVPLKMLPFDNK
                     NELLLVLDLDEGTTLERTDAAVRDMERYLAGVPELTDYASYVGLASPMDFNGLVRHYY
                     LRQAENLAEIRLNLVGKKSRGQQSHALGLRLRDDLTAIAARHGAKLKIVETPPGPPVL
                     STLVAEISGGPDQSYADLMRAAEVVGERMRREPFVVDVDDVIEADRSKLLFVTDQEKA
                     ALHGVSVRDIADTLQIALAGRQAGTLAMPNERNPLRIQLRLPRPARSSMADLGQIHVK
                     TAGGGLVPLAELGQWKEQPVDQTIYHKDLEPIVYIYGETAGRAPADCIVDIQADRRQA
                     APGESVSRGWVADVAPRPVDSRTFLSNGSGIHWQVPEGLRVNFAGEGEWQVTLDVFRD
                     LGLAFGAALVGIYILLVAQTRSFVIPVVVMLAIPLTIVGIMPGFWLLNALAGGQVGAY
                     ADPIFFTATGMIGMIALAGIVTRDSIILVDFIHLSLRRGRSLFDAIMESRVVRLRPIL
                     LTAGAAMLGAAPITIDPIFSGLAWALIFGLLSSTVFTLFVIPVAYWLLYAGKTGHGVP
                     EAEEDGGGDSNDEKEPALTVV"
     gene            complement(19330..20751)
                     /locus_tag="PLU99_00595"
     CDS             complement(19330..20751)
                     /locus_tag="PLU99_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO15579.1"
                     /translation="MRSDLPDGLAGQEPDVPAGHDPGRRGRAAREGATTRRRSLRARA
                     AGLRRDLLASAVDGAAYGGMLGLGHRYLPAFVLAVGLSEQLSGLVVTAPLVAGALLQL
                     VSPYAVVWLRSHRRWVAGCATVQGLSFLPLVIAALRGHVSAAALLMIATFYCAADLAC
                     AAPWTSWITRLVPTRIRPRYFARRNRLFQLVTLGGILMAWPVLELTEQRGATCAGFAL
                     LFGLAGICRLVSAGCLWLQRESIVPVAPERFVAFGEFFTRLRNERGQLLAYMVWINVT
                     MLVAQAYFTAFVLRHLHMPYWQFVSLLGTEFLAKIAGLALFGELAHRRGARALLQVAG
                     LAMVPVTALWLMWPQFWYLVAVQVLSGLALGAYELATCLLVFDTIPDRERTSVMTTYN
                     LLNSAAVAAASFGAFVALGYAGATWVVYAGLFVALTLARGLAVPLLARIVRARRPGEL
                     AAVGAAPSSAEAIAQARLWRSSR"
     gene            20974..22731
                     /locus_tag="PLU99_00600"
     CDS             20974..22731
                     /locus_tag="PLU99_00600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOO15580.1"
                     /translation="MPVDTAVMDKLTALCKRRGFIYQSSEIYGGIGGFWDYGPLGVEL
                     KRNIKNQWWHDMVTNPPIGPDGREVEMVGVDCSIIMNPKVWEASGHASGFADPMVDCR
                     ECKARFRADQVIVLDIYAGEDYLTSESIEANSSADPSIEEYRRRAAFVEAVAGGPADP
                     ELKKGAKKFRQALRDTVMSNQNLIDVVVRASGGAVTRDEVPNVLKSIKLNLRPRKLGD
                     QGPANARVACPRCHGVDSLTEPRAFNLMFQTYVGALQDESAKAYLRPETAQGIFANFK
                     NVLDTARVKVPFGIAQVGKSFRNEINPRNYTFRSREFEQMEIEFFCHEADSMKWYEFW
                     RNVRHSWYTNLGLRSDKLRLRDQGPEELAHYSKACADIEYLFPFSDEPQELEGVAHRG
                     NFDLTQHAKFSGKDLTYFDDEAWERDKAKFGGDKEAEKKAKNEPPYRFLPTVIEPSAG
                     ADRAALAFLCEAYTEDQAPDEKGVMQTRVVMKFHPKLAPTKVAVFPLVNKEGMPDVAA
                     AIYRDVKKHMTAFYDEKGAVGRRYRRQDEVGTPFCVTVDGQTLQDGTVTIRDRDTLAQ
                     VRVSKDQVLPWLQEKLGGC"
     gene            complement(22980..24434)
                     /locus_tag="PLU99_00605"
     CDS             complement(22980..24434)
                     /locus_tag="PLU99_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HOO15581.1"
                     /translation="MTYAKRESRTRVLSVVGTLLGGTLLALPLLAQVKPAAGGGEQAA
                     GAAERGTSSYMPVDLDIDFDAMMKRLQAEKPQIMQRQMQLLQERYDLSDNPAKDVTMF
                     RGKPIQQGVRVKLHGGVTWDDLAKMTPDEIREKDLWPKGFYPLPHPNHEAGGMVFPQF
                     QIDELKRQGLHDLTRFDLDFDIPDHFLAEFPPAIFLTTRPDLGDVSQGKLVTQKNFFE
                     LFNGILNPKQLEGLRLLVSQFPQQQFNFTNDRRSAEPHAGVTCFDCHANGHTNNATHL
                     VGDIRPQAFRHRIDTPTLRGVNIQRLFGSQRALRTVEDFTEFEQRAAYFDGDIVIAQK
                     KGVNFLERGSEVHAMAEFQELLDFPPAPKLNIYGKLDPQKATESEMRGQALFFGKAQC
                     AVCHPAPYYTDGLMHNLRTERFYEPKMINGRYATADGPIKTFPLRGIKESPPYLHDGR
                     ALTLEDTVEFFNLVQQLQLNEQEKKDLVAFLRAL"
     gene            complement(24964..27669)
                     /locus_tag="PLU99_00610"
     CDS             complement(24964..27669)
                     /locus_tag="PLU99_00610"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOO15582.1"
                     /translation="MRRSRSLIASCFRRYPKTFLAFFALLFMGLYAHASIYGTPQGLL
                     AQAPPAAETAAHAGEHHFQWWEVMRNPDFALGEKIALVGALVVAIGALVYAYSLVNFV
                     RRADTGTKRMQDIADAVREGANAYLKRQLSTVAVMILILIGLLYLTKAVQDHDWMSPF
                     AFGRAGAFFMGALFSATVGFVGMRLATTGNLRVAAAAPVGFGRALMLGYRTGTITGML
                     TDGLGLLGGTLIFMVYGERAYEALLGFGFGGTLLALFMRVGGGIYTKAADVGADLVGK
                     VEKDIPEDDPRNAATIADNVGDNVGDCAGMAADIFESYEVTIVAAMILGWASFGHKGV
                     IFPILVRAIGVIGSIISTYSVRAGDRGDVAEAMRSINKGFVLGSLISVTGFIILGFFY
                     LRFNADMVAAGTLSDVAWNSISTLPWYFNWGIEGLDMRPAITCFIGILLCIALNRTTE
                     YFTGTEYSPVRGIKRNCATGHATTIIEGFAVGYESAVWTALVLCIAIFLSVLVYAGTN
                     AIFIAFGVAMCGIGMLTLTGNTISMDVFGPVADNANGIAEMGYDPEQMGAAKYKEARQ
                     ILMDLDAVGNTTKAVTKGVAIGSAVIAAVSLYASFITVIGSGGQSEDKPLPIALFNHV
                     AALLTVSNPKLLVGMLIGGAVPLLFSGMLIRAVGRAAYLIVNEVRVQFRDKAIWEGTK
                     KPNYGRVVKICTAAAQHELIGPALLGVFTPILIGFLLGPIGLAGFLGGAIVVGQLMAS
                     FMSNAGGAWDNAKKMVEDEPRDLERNTGKGSEKHKASVTGDTVGDPLKDTAGPALNPL
                     LKVMNMVAILGVPLMVAYDAGVVRTVREGAAQAAQKALEAGKEFAFTLPESFQPKSID
                     GVWIGAVLVSLVLIIWAVWQSKRQAPEMAAMETETTKPEPVGAAR"
     gene            28050..28805
                     /locus_tag="PLU99_00615"
     CDS             28050..28805
                     /locus_tag="PLU99_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO15583.1"
                     /translation="MSGHSHWARIKHAKAVTDSRRGRLWSKLSRAIIVAARHGGGDPA
                     MNLTLRYAIDAARDANMPKDTIERAIKKGTGAGGEADYVELVFEGYGPGGTAILCTAL
                     TDNRNRTAPEVKKIFEGRGGKLGTAGSVSRMFNQRGVFTIAATAADEDTLTNIALETG
                     ADDVQAVGDEAFELTCDPAVFGEVRKALDNANIKPESAAISMVPITTVMLSGDQVEKM
                     MNLIEALEEHDDVQNVYTNFEISDDDAKRLAEA"
     gene            29152..30369
                     /locus_tag="PLU99_00620"
     CDS             29152..30369
                     /locus_tag="PLU99_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOO15584.1"
                     /translation="MPRWSAHVLLGFLFGALFSCNEPTANLEPFKAPVGKDYSRPLPP
                     GALALRKIGPEQYPDFSKGYHNLAGLEQAIQHSLNYLSKPSSQKYFPYGDISHARAVA
                     SLNHFLQVLHSVRSGAELDAAIRRDFDVYQSVGCDDQGTVYYTGYYTPIFEGRRQPDA
                     RFRYPLYGLPPDLVKGDEGVTLGRRTPDGRIVPYYTRRQITEGGLLDGTEVAWLKDPF
                     EAYVVTVQGSAKLRLADGSLYELGYAGNNGHEYTPIALQMVNDGVIGRNELSLQTMLR
                     YFSANPDKVNYYCLQNDRFVFFKELPGGPFGSLNVPVTPYRSIATDKEVFPRACLAFL
                     NTTLPTTYGGSLQIGPYSGFALDQDTGGAIRAAGRCDVYMGIGPQAEATAGRTGAEGA
                     LYYIFVKDGSGTL"
     gene            30832..32289
                     /locus_tag="PLU99_00625"
     CDS             30832..32289
                     /locus_tag="PLU99_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO15585.1"
                     /translation="MVALAERLSEDANIAAEDWPTAVYSIAELAERFDVSTKTIFRWR
                     RRGLVGWKFRYADRRMRVVFPDACVRRFVAQHAEIVSRGSSFSQLTRAERDSIVERAR
                     ALAGQGQSTVNSVARALAAETGRAVETIRLILKAHDESHPGAGIFNRSRLDVAADDQR
                     LRIWEAHQDGVSVAVLAERFNQPPSAIYQTITQMRARDMKSRKIEYIASPAFEEPGID
                     ELIANDPDVQAPYRPDTGGPRRIPADLPPYLQQLFRIPLLTTAGEAALFRKMNYLKFR
                     ADRLRQQLDPDEATASDLDAIDALLDEANAIKNQITQANLRLVVSIAKRHLGPALDFF
                     EIISDGNVSLMRAVDKFDYTRGFKFSTYASWAIIRNYARLIPEQRCQRDRYQTGREEL
                     LEITAERGADEQDDEQNALVRGVVERMLASLDLRERRILRQRFGLDEQQTPQTLEQIG
                     RHFGVSKERIRQLETRAIRRLRTDFALDVQRLLGS"
     gene            complement(32591..33496)
                     /locus_tag="PLU99_00630"
     CDS             complement(32591..33496)
                     /locus_tag="PLU99_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15586.1"
                     /translation="MWVYARQWRRKRQLDKASDVVCLLTPRSLERPWLLYEAGVAKGR
                     LDTPVYGVALGIPLSKASTGPFAQFQNCDDDPESLTKLVMQLVQRIPGSEPDHDAFLM
                     QVQIFKDKATELLSKRGQQDQKEQHAEADNTAIAKMFEEVKVIFRDLPTRVESALTDG
                     ERPVRKRRIFIDPIVLDDMSMFGQRLGNPAMGILVLASLLREETFGSAEELVQFLKNM
                     AEQESECDQSATCRYCGDAYDGRFADATSGRCPACALEADYGDVSASISSVLSRNDRA
                     ADVRERYHDSMPEGQRSGKRRTDGG"
     gene            34193..36688
                     /locus_tag="PLU99_00635"
     CDS             34193..36688
                     /locus_tag="PLU99_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15587.1"
                     /translation="MFSKALSAVAATLFLVPQIANGQSAPLSDGVYTRPESAAVAVEI
                     PESRLLAGVRAYPPGVLPALFTVSQQPAVIQQLAEDPQKLMNPQAISPPVSAELQNAI
                     RQLQQVPEAVALAAAYPDEIEILRQLYERAPQQTEQRLADLRARYDAARVQAGRAWQE
                     LLYENPGALDAYRALVTQFTRDQLKQYGDFPYVEVKQTRYYYAAPPNELIMAYAEDRK
                     PGAPLDAILQIWWNRYGPDQVDDLVFSQSDLAVGGSSVAAMSPERRAEMWQPARDRTG
                     SGNALLPIIMQPLPDQPADAQAAFAIVENARLWSGPQNDRLTQQPPVASPSPDMPGPN
                     AAPQSTGPQYSQPQYPAPQYPAPEAGESVLPEEPPAYVEQGPDYEYEYEYTTEVGVQP
                     QIVYTAPATVYYSPLVPYYYAYPAAWPVAYSCYPGLYTSWPYDWYAPGAYFSFSFGRV
                     PHYYRCGSAYWGRGYWGCGIWRGHGHWARPHHYYGQGYHGRDGYHVGRYGRGLDRITH
                     QFRGGERYGHGRDRISPIGRDARRGDRYGLADRIGALRSDRLGTARGDRSGIVRGDRS
                     ASAHGDRLGSVRRSATDRGERITGKDRSADGRSERLGGDRSAASRGGADQRSGIRGPD
                     RKVDRRSPEGSQILRQGSGGRSLERGQSTQQRGGTRSRDAIPGSVSGGSRSSKGAAGV
                     RQGAGRQSTQRGQALGGSSPSAGSRGTGSINRTPGAGRSSGVSRGTQSAPRSIAAPRS
                     PSTSRSPAAPRGSSTSRSPAAPRGSSTSRSLAAPRSSGGSRPMGTIRSGGGMRSGGAG
                     LHSGSGVRSGASGRQGAGSGLRSGGGLRSGGRR"
     gene            36998..>38044
                     /locus_tag="PLU99_00640"
     CDS             36998..>38044
                     /locus_tag="PLU99_00640"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HOO15588.1"
                     /translation="MLALIWLIIAGNFVAFADAPPPAERQLRGAVDTRVELMSIIFRL
                     AGNPEYNMPNSNSRYADEVEQHFGRLRNHPAVKYAQELRQKAGISYDAVMSLAVHTTD
                     ALELNEKIPFDQAPARLDARWKTAEAREFLKLARQFARDSGFRRFVRNHESYYNAVAE
                     RINEQLAQRDYLKWFDDFFGAQPQASYSVAVGLLNGGSNYGVGVRYPDGREEITPVLG
                     VAQFDEQGLPVFDDSFGPLLIHELCHSYANPHAEKYEKLLEPAGLALYARVGEQMKKQ
                     AYSNWKTMLSESLVRACVARYQLAAEGEEAAAKLQAQDAARGFYWIGDLVELLGQYEN
                     TRQEHPKLDDFMPDV"
BASE COUNT         7322 a        12148 c        11892 g         6682 t
ORIGIN      
        1 atcggcaatg tagccggttc cggccgaatc cactgcgatt acggtcaggt cggtagcgcc
       61 cggcacgatc agcgttgaat cgggcatcac cgaaccgttg agagtagccg cattgaagaa
      121 gacgttgacc cggtttgcca aagcccctcc gtcgccggaa ttgacgacgt agagggtatc
      181 gttggcatca acgaacacgc cgtaaggctt ggaaaacacc gggctgttga gaatgcgtgt
      241 cgcgttgacg acgccgttaa gcgtgctggc gttggcgaat actgcgaccg tgttcgcgtc
      301 aaaattggcc acgtacaact cgtcatttgc gccgaagcgt attccgaatg gcccgttcat
      361 gtccgtgctg gtgatgttgc gaaccggggc gaggtttccg ttgaaggccg cggtcgaagc
      421 acccgcaaac acatgtatgt tcccgccgat ttcatcggat acaaacagca ggtcggccgc
      481 ccggttgagc gtcatcgaag ccggccggtt caacagggtg gccgccccct gcacattccg
      541 cgtgggcgcg acgctcccgt taatttgacc aagggtcaga gcgttcggat acgacgttat
      601 ggagttgctg ccaaaattta acaccagcag gttgccggcg ttgtcaatta agaggtcatc
      661 aggcacggac agcagcgttt gggcgccggc caggtttgca ttgggcggta tattgccgtt
      721 gatcgcgctc ggcgtagtta tgtcgtacgc cgtaacggag tctccactga cgttggcgat
      781 gaaaaggatc ggcttggtca ggggcgtggt cgtcactgtg acggaggtag tggcagacgc
      841 ggtgccgccg ttgccgtcgt ctaccgtaag ctggaaagtc agtacttcgg atacgttcgg
      901 cgcggtaaag gacgcagtgt ccgtatcggc attatcgagc gtcacagtcg tgccggagat
      961 ttgcgtccac tggaaggtga gcggatcgcc atccgcgtca ctgctgccgc tgccgttcag
     1021 cgtgacggtg ctgccggcgc tgaccgtttg gtccggcccg gcattcgcca ccggcgattg
     1081 gttgggcggc ggcggcggct cgggcggaca gccgcacaga ctaagcaaca taatgagcga
     1141 agcaccgagc gccagcgcac tccacggctt acgtatacac atactgaatc tccgaaggcg
     1201 gccgcgcagc gcggccatta cccacacacc agcgtcttgt ggaaggtatc gttttactgc
     1261 gttcatgtac gatgcgaccg ggcagtgacg ccacaccgta gtgcagaagt ccggggggcg
     1321 gcactccggc ccgcctgtcg caggccggct gtgctgccct catcacatcc ggaatcaatc
     1381 gctgggccgc tattggctta ggaacacggc agcgggaccg cccagcagcg tgttttcgcc
     1441 agtcagcgtg cggtcgggcg cgatcgtgcc gttccgggtc gcaatattgt cgtacccata
     1501 gatcgcgccg gatgggccgg cattcgggtc agaaatgtag ccgttgccgg ccgcgtccac
     1561 cgtcacgccg ttcaattgca cggccccgag aacggtgagc gtcacgtccg gcgtcaccgg
     1621 accgttcagc gtagctgcat tcgcgaagac attgacgcgg ttgccgcccg ctgggccact
     1681 taccgtatat agcgtgtcgt tggcgtcaat aaatacatcc acgatgccgg caaagagtgc
     1741 actctggagc acacgggtcg cacggacctg cccattcagg ttactggcgt tggcgaacac
     1801 ggccaccgta ctgttgccga ggtttgccac gtacagctca tcattggcgc caaaattgat
     1861 accgcgcggg ttcacgaggt caatgctgag aatgttgcgc accggagcga ggttgccgtt
     1921 aaacgcattc gtggatgcgc cggcgaaaac gcggatggcg cctcccccca ccgcgtcggc
     1981 tacgaacaat aaatcgtttg tacggctgag cgccagcgac accgggttta cgagctgggt
     2041 tgcggcgcct tgcacgttcc gggccggcgc cacattgccg ttgatcaagg cgagattctg
     2101 cgcgctcccg taactcgtga tcttgggagc agcggtacta agcaccagta gcgcgccgtt
     2161 cgcgtccggc accaggtcga ttggaagagt cagcagagtc tgtgcgccgg ccaggttggc
     2221 ggttggggcg atgttcccat tcaccgcatt cgggttgctg acattgtaag ccgtgacgct
     2281 gttaaatgcg aaattcgcga cgtatagctt cggtgtattc gccggcggcg gggtcgtcgt
     2341 gaccgtcacc gtcgtcgtgt cagtatccga ccccagatta tcgctcaccc tcagctcaaa
     2401 cgtcagcgta gtcgcggcgt ttggcgcgag aaacgtggcg ttcgcggcgg tgccgttcga
     2461 tagcacgacc gcatcacccc cggtctgtgt ccactgaaac gccaaggggt caccatcggg
     2521 atccgtgctg ccgctgccgt ttagtgtgac gtttgtaccg gcttgcaccg tttgatccgc
     2581 acccgcgttc gccaccggca actgattggg tggctcgggg ggacagccag ttaaacaaat
     2641 gagggccagg gtcgataagc cgagcgcgaa caagctccac tgctgacgtt ggaacatctg
     2701 catacctcca gcaagaacac gcatacgcgt aaagtgtcag cccgaacaca gacggatgcg
     2761 cgagtacctg aaagagcgta cgaggctcgt atgcgaaatc aagccccagt ctgtaatagc
     2821 cgcgcagttg tatgcagtta tattgagact attgcgacgg gtattggtgg acataacgcc
     2881 ctcgtgattg ttgatatgac tccgcagtta cccgtcagcg ggagcggcgt gggattgacg
     2941 cattaggaag acaagtaact aaggccggtc agcactgggc tgggtggtcg ccgctcgcag
     3001 ggtctccagc cgcgactcga ccggtcgccg caggggatgt tcgggagata ggcgatccag
     3061 agtctggaca tacagtgaga gcccctcgtc tgaccggccg gtggcgatca atgcgtccgc
     3121 cagcaagagc gccgtctcag gatcctgcgg cagcagcgcg tgggcctgct gggcgtagcg
     3181 cagagcggcc ggcgggtcgc gctgcaccag cagcttggcc agaacggcgg gagggcgtac
     3241 ctcgtgcggc gccactgctg ccagcttttc cagtaccgga actgcctgct ccagctcgtt
     3301 cagcttgagt aacgcttcgc tcatcagtag cagcggggcg cggtcgtcgg gcgcgatacg
     3361 cagtgcgttt ttcaggtgcg gcagcgcctc tcgcgagcgc cccagtttca agtgtgcttc
     3421 tccgagcatc gtcaatacta cgtaatcatc gggcgcgagc gcggcctcct cttcgagata
     3481 gggcagagcc tcggcgaatt gacccgcctg gaaaagtcgc cgcccaagtc tgcggcgcag
     3541 cagcgtttcg ccggggttaa gctgcaccgc ctggcgaatg tgatgcaacg ccatgtcggc
     3601 ctgaccgagc aaactcagta tgtcgctgcg cattcgatga ttaccccagt gcaccgggta
     3661 atgctggagc gcccgggcag agtattcgtc ggcacgctcg agattctgcc gccgcgcagc
     3721 cgggtcgtcc gtctgtaacg cgacgtcgat atattgcagc gcggcaccgg cgttaagcgc
     3781 tgcgctctga gggtagcagg cggcgtcgtg cgtggccagc cgcaaagcac tgtaccaatc
     3841 cgtgttgcgg atgaccgtac gtgtcgcaaa cgctgccagg actagtgtgc cggccacggt
     3901 tgcgaacctc ggagcagcca gattcgctcg cgccgcgccg gccggccgcc gtccgagcga
     3961 caaggccaaa gccgccagca ggccgcaggc cgcgaccgat ggccagtaca tcagccgctc
     4021 cgcgacggac acgccgatca acagcaacga gttcgagaaa agtgcatacg cggcgatgaa
     4081 cagggccgca cagaccgcca gccgccgcca atggccgccc gcccgcaagt agccgagcag
     4141 cgcggccaca aggccggccg ccgccgccaa ccccagcagg gtcatggcgt tcactccggc
     4201 ccgcgggttg aacacttcgt atccatagtc ggcgctcaaa tgcagcggaa agagcagcag
     4261 acgcacataa tgtcccagca cctcgaatgg catcaggagt cgcgccggca tttccgcgtc
     4321 gacaatcggg ttaagcaatg ccgccggggc acgcatgaac gcgccctcca aggcccagac
     4381 ccgcagtcgc agataaacca gcagcggaag cagcagacac agggtgtgca cgccccacca
     4441 gcgcacgcca cgtatcggcg tacgcccgcg gcaagcgtgc aacacgatta aggccatcgg
     4501 aatatagctt acggccgtct ctttggagag cagcgccaaa aagaacagcg gcatggcacg
     4561 ccaggcccgg ccggtgtcgc tcggacgatt tttcggaacg agtacgagca gtcccaagag
     4621 cgaaaagatt gtggccagca cttccgcgcg gccaacgatg tatgccaccg cctccacgtg
     4681 caccgggtgt acggcaaaca gcagcgcccc ccaagtcgct agcgagcgat cctcgaacag
     4741 ccgccgcaga aactcccata tcagaaaaca cgtcagccca tgcagggcaa tattcaccaa
     4801 gcggaacccg ggcgcccaca gaccgtgtaa gctgtgaccc aacgccagcg tgaataccgt
     4861 gaacggccga tacagcaggt ccggattggg gtgcatccgg gcctctgccc cgctgtacgt
     4921 gtttgtccag ccgctgagcc agatggcatg gaagttcgta attgagcgga ttgcggggtt
     4981 ctcagccacc tgcgggacat cgtcgtaaac gaaaccgttg ggcagactgt tgaggaagca
     5041 ggcgcagccg accacgaccg gcagccaagc ccgatggaac cacgccgcgg cggcgggggc
     5101 ttgagtcgcc tgcaagcttt cgcagctcga gcaaagcagt cggccgggcg cgcccgattc
     5161 aatcggtcgt tggcaacgcg cgcactgcgt cgcgcgttcg ggcatgcccg cggcgatggg
     5221 cgcatgcttg gcgggggaca ttgaactctc caacggcgcg ccacgttgcg cggttcaacc
     5281 atagtacttg cccggtcacc gtgctgcacg cgccataggt cccagaaccg cgccggcagg
     5341 tgcaagagtg tcggcccgtc ggcccggcct cggcaaacgc gagtcgcccg cctgttgaac
     5401 cagcccggcg gcgaagcagc ctgtccaagg agagcgagcc cgcaacggcc gctaaaaaca
     5461 gccgcggcgc aacgctatcg cggccctgcg ctcgccgggg aggcagcacg ctaaagttgt
     5521 ggccccgatt gccgttttcg actattattc aggcgctgtg accgggctcc aggcggtggc
     5581 gataacgcag gcgcctcgcg gtacggataa ccgctgccgg cagccctccg ggaagattcg
     5641 ataaactgcg catggcggcg attgcatgcc ttcgaagctg gacaccgcgc tcggcgaata
     5701 cctgaagcag ataaagaact accccttgct cacggccgag gaggagcgcg agctgggcgc
     5761 gaaaatccgc gcgctggccg aggcccagga gcaccaggct gagaagaaca ttccgcccga
     5821 aacgatggag cgcttgcgcc aggaagccgc cgaggcccgc gaacgcctgg cccaggcgaa
     5881 tttgcggctg gtgatcagcg tcgcaaagaa cttccgcaat cgcggtctgc cgatggagga
     5941 tttggtcaac gagggcaacg tcggcctgat gaacgccatc gaccgcttcg atcccgcggt
     6001 cggctcgcgc ttcagcactt acgcgggcta ctggatcgac cagtccatcc gccgcgcggt
     6061 gcagaacgcg tcgcagatga ttcacatccc cagctacctc atggagcaga tcggccagat
     6121 gaagctggcc atgcgcgaat tggaagagaa gctcggccgc cagccgtcaa tgaacgagtt
     6181 ggccaaacac atggacataa cgccccgcaa ggccgcggcc atctcgcaag ctatccgcgc
     6241 ctgcacgacg cgcacgcagg attcgctggc cgagggcgag acctcgctga ccgaggccct
     6301 gcccgacacg cgtacgccgc agccgtacca ggccgtgttc tcgcaatccg actcggattt
     6361 tgttacgcgg gtcttgtcgc gcattaccga gcgggaggct ctggtattga agctgcggta
     6421 tggtctgaat aggcgtgacg gcaagcgcat gacgctgaag gaaatcgggg aggcggtcgg
     6481 gctgacacgt gagcgagttc ggcaaatcga aaaggacgcc gcccgcaagc tccaggaata
     6541 cgttaacgag tactactgac gccactactg acgccgcggc gtcacggtcc gaaaaagtac
     6601 gctcaaaccg tttttcggcg caaatcgtcc gcatcaattg gggttgttat caaaaattaa
     6661 ctaaagccga gtcgcctgga ctcgtgggat ttcgtattct tttgcagagg gacggaaagc
     6721 ggtagcagca ccccgctttc ctcgttgacc gcccaatcag tagcgggcgg gtcgcgataa
     6781 gccttttgac aataagaact tgtgcgcaga tttgcgcgct tggagtttcg ccatgctgac
     6841 tatgcgtcgc acgagtctcg ccgcaggtac acgcgtaaag gtgaaaacgc ccggaccggt
     6901 gccgcactgg tccgtttggg agccctgcca acgaacctcg agccacgtga aaaagcggct
     6961 gcaggacatg ttcttcaagg gcgaccggcg aattacggct gaagtagtgt acatcgccag
     7021 cgaatctgat cgggagaagt tgaaggcggc gggccgcgta aagctatacc tgcgcgacgc
     7081 ggccggttcg agcgtcacga tcctggccga cgccaacaac ctcgcggcgg cgagctaggc
     7141 cggccggacg gcgcgcgagc ctatccgcgc gccgaagcgt ggccgcaacg taaagctgga
     7201 tgtccgccag actgtagctg aatgtccgcc aacacggcgg ttaacaatca tgcaccgcgt
     7261 tcgagtcgtc gtctcctgac tcggacgcgg tgtgtggcac ttatcggatt ttctgaccca
     7321 ttttagtgcc gatacaatcg cgcgccgttc ctgacggatt tccgtgcgcc cgaatcggcg
     7381 cgaccaaagc agagccgggg cgtgccgccc cgaaatgccg ccaggaatct cccgccggcg
     7441 cgctacgcgt tacgactgca ccgtgccgtc ccaaaatggc ggcaacaact cagcgtcaat
     7501 ccgaggccgg agcgagatcc gcgcccaaag cgccagtgag cgcaaccgat ccgaacctgc
     7561 acgtgatgcg agccctctcg atccgagcct tcagcgagat tcgagcccgc agcgccagcg
     7621 agggcgaccg atttctgatt ggagtcccaa ccgctcgaca acgccacttg atccgacccc
     7681 gcagcgccca cccggggctt gcaccgtgct ccagcgccta tatagccgag atgtgctagc
     7741 agtcgggatc agcactgctt gaagttgcgg cttcaagcag tggcacacac accgttggca
     7801 gcgcggttcg gacagtcgat gcgttcgcag cgcaacagag ccggggcgtg ccgcccggaa
     7861 atgccgccaa gaaccttcca tccgcacagt ggttcgcccc gcccctggtg tatcggcccg
     7921 aaatgccggc aactccgcca ccagcggcat accgcttcgg cattttgccc atagctgcga
     7981 acgaaaaata ttgaatccgg cccggcgaaa acttacgatg acgttcagtc atcgggtgcg
     8041 gcgtccaagc gcctttcgcc ctttctctaa ggaggagaag tcacggagcg catccctgca
     8101 actgggtcat cggatctctc cacgtgtagg gccggctctc cggacccgcg aggcgtctag
     8161 cgaaggagga gttttatgaa gcgaatagtg gcagtcgtgg cggcaggttt gtgtgcctgc
     8221 ctggtgcaag cagaactgtt ctggatcagc tatgacgcct cgtgcgggct gtaccccgaa
     8281 gaggtgggct ggacccgcac cacacacggc ggcggcgcgg tgcgctcgct ggccgacgga
     8341 attctcacgc tcgactccac cgctgacagc atgatctgcg atggctatgg catttaccgc
     8401 cccaccacgc cggggccggg cgagacgttc gtgtgcgagt ggcgcatgtg tctggcctac
     8461 cattcgggat tcggggaaag ccttttgtct atgcaggccg acgacggcgg cggcgtgcgc
     8521 ttccggtact ggatggatca ccttcagagc gaagagggct ggatcgtccc gatcacggcc
     8581 ggcgtcttcc actcctatcg cctcgagtca acggacatgt acacgtattc gttgtggcta
     8641 gacggagtgt acatgggcag cgacgctttc gatcggcccg gcccgccagt tccgtatgcg
     8701 gcgttcggcg actgcacata tgggacgaca ccgcagtcgt cgctgacaca atgggagtat
     8761 ttcaggttcg gcgtagtacc agaaccaagt tcgctggcac tagtgctggc gctaacgact
     8821 tgcgcgtgcg ttgcgcgcag acgccgcgcc agatgggagt cttgaaagga gatgtgttat
     8881 gttgagtaag gtgtctgttc taatgtcgtg cgcgctatgc gccctgccac tcgcgcaggc
     8941 aaatcaagtc agggtttggc acgcgaccgg atcttacaac atagatcccg actttcatac
     9001 ggtcacgatc accagccccg gtacgttcaa aatccaggcc acggacggca gcggtgggct
     9061 gggctacatc gccaagatcg acgtcgcatt cgccggcctg cccggccagg tgatcgtcta
     9121 cgtcgtacgt gacccgagcg aggtcggtgg ggcccagcat gagcctggcg ccgccgaggt
     9181 ttgggcgatt gacctgtcgg cctgcgaaac ggccggcacc accggcactg ttgccgagct
     9241 gcgaacgacc ggcaactacg gccacgtcac ggacggagac acgctctacg cctccgatgt
     9301 cggcgcggtc ggcggcggat cgaggcgctg gatgagtgca agccaggggc tgtcccgatg
     9361 ctcacggcac gagtcatttt gaacgccgtg tgcgtgggtg ccgggatgtt cctggaagcg
     9421 tgacaagtgg ccacccgact tatgccggcg gcgcggccgg caagaaaacg tgcgcggtcg
     9481 tcccctctcc cggctggctc tccagccaga tatgtccgcg atggctctca actatgcgat
     9541 aggcccgcgg cagccccagc ccgcggcccc ggcccgccgc gcggtgcgaa aagaatggat
     9601 cgaacgcccg ttgcagcacc cgcggcgaca ttccacatcc atcatcgcgc acgataactt
     9661 caacacctgc agcccccgcg gccggctgac agactatttg aatagcgccc tctgaatcgc
     9721 ggattgcatg ctgggcattg ctgaccagct cgtcaaagag aactcgcaat tgctcgcgat
     9781 cagcctgtat gttcagggcc gaagaaggcg atgcggccgc gcaagggccc cgttggtcga
     9841 tgctgagcag gagcttggcg cccgacagct tccagcgcgt cagccatacc tggcgtagct
     9901 ccgccagtag ttccggcacg tttaccgggg ccagcgtcgg cggacgcggg cgcgcgaaat
     9961 ccatcagctc tgaaacgacg cggctgcatt catgcgcctt gctgcgaatc gtctccaggc
    10021 agcgtcgcgc ttcgggatcg ttgagttgtg cggccagcat ctgggcccgc cccgaaatca
    10081 ccgtcagcgg gctgttgagc tcatgcccgg ccccggcggc catctcggcg atcatcgaca
    10141 gcgtccgcga acgcaacaat tcggcctggg cctgctgaag gttgcggttg gtttcggcca
    10201 gatcgtcagc caggcgacgc gccgccgctt gggcattagc gcggcacaga gccaggccga
    10261 ggctggcggt aaacgagcgc aactcttctg cttcaccggc caggcgggcg cgctcgtccg
    10321 tttcggagcc gtaaacaatg ccgccggcca agcgagcctg atacacgatc ggaatgagcc
    10381 aggttcccgt gccgctgatt tcgtgcagcc ccggagccat gagcgtgcgg atcgcggtcg
    10441 gagcgcgcgt caccagcgag tcaataacgt catcagggct gttcaaccag tcccgcacct
    10501 cggccggcaa gtcggcgaaa gcaaccgagg tagtgttctg gcgggcgtcg atccagcaga
    10561 cgtccacggc gggagcccgc tcgcgcagac caaacgcagc cacggcgggc cgttgcagga
    10621 ctatgcccgc caccgcagcg atggcggcca ccgccttggg caagtccgac caggcgccca
    10681 gcaagcgatc aaattggccg agggcgcgga agtagcgtgc cccggcggcc aggcgttcgt
    10741 ttgccagggt caactcggta ttgaggcggc cctgttcggc gtcggtccgc gacaacccca
    10801 gcgagtggac gccgccgttc gccttatgct ccaagcccag ttctttcgtt agctccgcga
    10861 cttcctggtt cagtcgggtg gcggtttcgg aaagctggcc ttccgacagg cccagccgct
    10921 ccgccagccg cggtgagtgc tcgtagaaga cgtggttgcc cgaatagccg atacgccgct
    10981 cgcgggccag cgtgtcagcg aggagcacca gggcgagaag ctgccggtta gcgactgagg
    11041 ccggcaacgc gtcgccggct agatggtgca gccagatcac atcccgcagc aggcggggca
    11101 aatgccaacg ctcagccaag cgccgtccgg caacggtgtg gtccgttccc aacagctcgc
    11161 gttcgaaatc ggcgatgtcg ccgcaggcac ggttagcttc ggccgcaata cgctgatacg
    11221 tcttcgggaa gaccgcactg agcgcgacct tgcccaggtc gtgcagcacg cccgcgacga
    11281 agacctcctg cggatcgacg ctcaaggggc gcagcgcctc ggccagcgcg cgcgccgcac
    11341 atccgacggc caatgagtgt ttccaaaatt caggcgggtc gaaggttgcg tcgtcgccgg
    11401 cgggcctgat cggcagacaa tcaaagacgc tcgctgtcag cgctatgctg cgcaggcccg
    11461 caaagccaag atgcggaatg gtttgctcca gcgtgtcgac cggtccgggc aagcgccccc
    11521 aagccgagtt ggcgaccgac agcactttgg ctgtcagaga ctggtcggcg cgtaggatct
    11581 cgaccgcatc agccaccgaa gcgcgcggct ccgcggccat ggcgagcagt ctgaccgcag
    11641 ccgaagagag ggccggcaaa cggtcgaggt gcgcgagtgt gaggtccggg tccggcctgg
    11701 cgcccgggct agactccaga cttggttcgc ttggctcgcc cactgaccag ttccgtaatt
    11761 cgatttacaa gttgctcgat gctgaatggc ttttgcagga agtcgtctgc gccggcctcg
    11821 aacagcttgt cgacgtctcc aggatcgatc gcgccagaaa caaagatgat ttttacatct
    11881 ttgagcgatt cgtcgctacg gatgctgcgg cagaccgcgt tgcctttaat gtcgggcagc
    11941 aggtagtcca gcacgatcac gtcgggctgg aattctctgg ttcgcagacc ggcgtcgaaa
    12001 ccggttgcgg ccgtctgcac gataaagcgg ccgtctctct ccaacagctc gaccagcatt
    12061 tcgacgatgg ccgggtcatc gtcaacaacc aggacccgcg tccggcccat ctcgagccgg
    12121 tcaagcggga tattgttctc cttcatgaac tgaatcaggg catcgcgcgg gatgcgcctg
    12181 aatcgggagc ccggtatgcg aaagcccttc agacggccgc tgtcgaagca gcgaatgacc
    12241 gtctgctggc tgagactgca gatctcagcc acctcgcccg tggtgaagac agtcttcagg
    12301 taacctgtct tgtccctgtt gttaatagcc atagcccaac cccgggcgtt tttgcctatc
    12361 tttcccaact tcacaagtgt accgcaaaag agaatctggg gcaagtgaag acgggtggcg
    12421 gcacacgcat cgtgtaggat tcgacaatgt tttcgatcga tccgtctagg ttataggtct
    12481 aagattggcc gcgaactcaa ggccagagtg agcgaacaac cagccgctaa acgtatgacg
    12541 cacgcccgaa accggtcacg gtcgcgcaaa aatcctcatg acggttttgc gccacgaccg
    12601 ggcgtgccga cgctatccac agctcgcgcg ccgctggccg gaggcaatgt ataccagaag
    12661 cactgacaga tcggtggggt gaatgccgct cacccgcgcg gcttggccga tgttttgcgg
    12721 acggaccgcg gaccagcgct cgacggcttc gcgccgcaag tgtggcactt gcgagtagtt
    12781 cagatccggc gggatgacct gctcttcccg gcgccggaat tcgtcaatcg accgctgctg
    12841 ccgctggaga tagccggcgt acttgatttc cacaagcgcc cggccccacg tgtccagttc
    12901 gcgcgtgtat ggctccagct ccggaaatac cgccgcgaag cgtagcccgt cctcctccgg
    12961 ccggcggatc cagtcggcca gcgtccggcc ctcgaacctc aagcgttccc aactttgaag
    13021 cagtgccgcc agcttgtccg acttggcctg gaacttctgc caccgctcat cgtcaacgag
    13081 gccgatttcg cgcccgagtg gcgtcaggcg cacgtcggcg ttgtcgtacc gcaggtgcag
    13141 ccggtgctcg gcgcgtgagg tgaacatgcg ataaggctct gtcacgccgc gagttacgag
    13201 atcatcgatc atgaccccga tgtacgcctg gtcccggccg atgatcaccg gcgaggcgcc
    13261 agcgcagaag cgggcggcgt tgataccggc gatgatcccc tggccggcgg cttcttcgta
    13321 gccgctgctg ccgttgatct ggccggccag aaacaagccc cgcacccgct tggtcatgag
    13381 gctggcgtcg atctgctcgg gcggaacgaa gtcgtattcc acggcgtagc cccattgcag
    13441 aacgcgggcg tgctcaaggc cgcgaatcga cgtaactatt tgtgcctgca catcttgcgg
    13501 aagcgacgtg ctgattccat tggcgtagat gcgctcgctg tcgcgtccct ccggctcgag
    13561 gaagacctgg tgggcgctct tgtcggcgaa gcgcacgacc ttgtcctcga tgcttgggca
    13621 gtagcggggc ccgcgcgact gaatctggcc ggtatacatc ggagcgcggt gcagattagc
    13681 ccgaatcagc tcatgaacgc gctcgttggt cgcggtgagc cagcagcaga cctgctcctg
    13741 tggcagcgag tcggtgagaa aggagaaggg cacggggcgc tcgtcgccat gctggacggc
    13801 gagctgcgac cagtctaccg agtcgcgggc aatgcgcggc ggcgttccgg tcttcagccg
    13861 gccaagctcc atacccaaag ccgccagtga cgcgctcagg ccggttgccg ccggctcgcc
    13921 cactcgcccg cccggcgcct tggcttctcc ggtgtgcatc aggccgcgaa gaaacgtccc
    13981 ggaagcgatc acgacagcat gcgcggccag gacgcggccg tcggaaagcc ttacgccgga
    14041 cactcggctg gttgcgagcg gaagggcatt cgcgcaggag ggcccgagcg gctcgctttc
    14101 gacggcctca acgctggctt cgataatctt caggttcggg cagttctcca ggtagcggcg
    14161 cagcactgcc gggtaggttc cactatcgat ttgtgctcgc ggcgcccaga cggccggccc
    14221 ttttcggcga ttgagcatgc gaaactgaat gccggtctcg tccgccacac gccccatcag
    14281 gccgcccaag gcgtcgacct cccgaacgat ctgccccttg ccgatgccgc ccaccgccgg
    14341 gttgcacgag ggccgcccga tcgcttcaag actgaacgtg atgaggaccg tgcgcgctcc
    14401 caaacgcgcc gccgaccacg ccgcctccgc tccggcatgc ccgcctccca ccacaatcac
    14461 gtcgtagccg ctacgtctca ttacggggat attatctgac agggccgaca gagtgccagc
    14521 aacacgctgc caccacgggc ccgttacagc cgaggaccct tcgaatgact aagaccgcgg
    14581 tttcgccacg ttcatgggcc gtagcgcgac aagtcgcgtt agggctcggc ttctcagccg
    14641 tcgtagttct gctgctctta tggctggccg gcacctttca ccgaaagatc ggccccggtg
    14701 ggccggagcc agcgagccgc ccggtcaggc tggtcaaggt ggatcagctc gttccggcgc
    14761 gcctcgcgtc cttaccggtc accgagccgg cggtcggatc gattcgcgcg gtacaccaga
    14821 tcgcggtagc ctcgaagctg ctggcgaagg ttacggccgt caacgtgcag gccggccaga
    14881 cggtcacagc cggcgaagtc ctcgtgcaac tggatgacga ggacctcagg gccaagttgc
    14941 gtcaggcagc ggcaagcgtc gccgtggccg agacggccct gtctcaagcc caggtggaat
    15001 tcgagcgcat cgacggcctg tacaaacaag gaaacgagac gcgcatcgaa tgggagcgct
    15061 cggaatcggc cttgaaaacg cgcactgcgg aactcgagga agcgcgtcag cgccagcggc
    15121 aggcggaaac gatgctctcc tacgccacga tcgtcagccc gatcgatgga aaggtgatcg
    15181 acaagcacat tgaggttggc gacacggcgt caccggggca ggtcttggtc acgctctttg
    15241 acccgacgcg catgcaactt gttgcccggg tgcgcgagtc actgacacat cgactggccg
    15301 tcggccagga tatcgacgta tatgtcgacg cgatcggaaa gacctgtacc ggctcgatca
    15361 gcgagattgt gcccgaggcc gagagcacca gccgcacctt ctccgtcaaa gtgaccgggc
    15421 cgtgtccggt gggcgtgtac tcgggcatgt ttgggcggct gttgattccg ctggatgagc
    15481 aggaagtgct gcttattccg cgcgccgccg tctcccggat cggccagctc gatctggtgg
    15541 aagtcgcgct gcaggatcag tcattgaccg agcgcgaggt agtgctaaag ggacggccgg
    15601 tcgagcggcg ggcggtgcaa ctcgggcgga cgtacggcga cgaggtcgag gttctatcgg
    15661 ggctgcgggc cggcgaactg gtggcattgc aggggtcggc acaatgacaa tcgacaagtc
    15721 gcccccgccg gaagtacagc acggcttcac taatgcaata gtccgcactt tccttaattc
    15781 aaacctttcg ctggtattga ttttcatcgc catcgcgctg ggcgcggccg cgctgatcct
    15841 cacgccccgc gaggaagacc cgcagatcgt cgttccaatg gccgacgttt atgtggcttt
    15901 tccgggtcat tcggccgcgg aagtcgagca actggtttcc acgccgctgg aaaaactgct
    15961 ttatcaaatc gacggcgttg agcacgtgta ctcgatgtcg cgcccggacg aggcgatcat
    16021 taccgtgcgc ttttacgttg ggcaggaccg cgagcgcagc ctcgtcaaac tctacaaacg
    16081 gctggacgag catctggaca tcgtgcctcc cggcgtgacc gggtgggtcg tcaagcccgt
    16141 tgaaatcgac gatgtgccga tcgtcacgct aacgttgacc agtgcttcgg ccgactacta
    16201 cgcgctgcgg cgcgtgggcg aggaggtggc ccagcggctg gcggcggtgc cggacacttc
    16261 gcgctcctac gtgatcggcg gtcaaccgcg ggcggcgctg gtgtatctcg atccagagcg
    16321 ccttcaggct tacaaccttg cgccgctgcc cgttcagcgg gcgcttgaaa ccgcgaacgt
    16381 gacgcagacc agcggcgaat tcgcacgcgg cgaccacctg tttcgggtcg aggcggggcg
    16441 gatatttcaa cggccggccg agctgaatga cctggtcgtg ggcgtttacg gcgacaggcc
    16501 cgtgtatctg aaggatgtgg ccagcgttca ggacggcccc gatgagacga cgagctacgt
    16561 gcggttcggc tggggagcgg cgcgcgggtt tgagcagcat gcgggcgcgc cgggcgctgt
    16621 cgtcggcgcg tcggcgtcat caccgggttc cgccggcgca ggagagcccc agccggcggt
    16681 gaccattgca ctggccaaga agaagggcac gaacgcagtg tgggtcgcgg atgcgatcct
    16741 caaagcggcc gatcaactga aggccggcgt cattcccgac aacatggaac tgatcgtcac
    16801 gcggaactac ggccagaccg ccaaccacaa ggtgaacgaa ctggtcgaag cattggcgat
    16861 cgcaatcatc atcgtcatag ccctgctgac gctgagtttg ggatggcgcg aagccttgat
    16921 cgtggcagtc gcggtacccg tcgtgttcgg cctgacgctg gctgtcaata tgctcgctgg
    16981 ctacacgatc aaccgcgtga ccttgtttgc gctgatactc tcgctcggtc tgttggtcga
    17041 tgaccccatt gtggatgtgg aaaatatagc ccggcatttc ggtctgaggc ggcgggccag
    17101 ccggcctatt gtattggaag cagtggccga aattaggccg cccctgatta ctgccacgtt
    17161 ggccgtaatc gtgtccttcc tgccaatgct gttcatcacc ggaatgatgg ggccgtatat
    17221 gcgcccgatg gcgctgaacg ttccggtcgc gatgttgatg tcgatggtgg tcgccttcac
    17281 gatcacgccg tggcttacct tccacgctct gaagcggcgt tattggggag gggatcagcg
    17341 gatcaaccag tcgagggatc aagaagcgcg gccgcagaat gaggagatac acgatctaga
    17401 tgcactgcgg cagacgcggc tgtaccggat tttttatcca ctcatggcgc cgctgctgaa
    17461 gtctaagaag gccgcctggg gctttttggg cgcgatggcg ttgttgatgc tggcggcggt
    17521 ggcattggcc gccacgcgca gcgtgccgct caagatgctg cctttcgaca acaagaacga
    17581 actgctcctg gtgctcgact tggatgaagg cacgacgctg gaacgcacgg acgccgcggt
    17641 gcgcgatatg gaacgctacc tggcgggcgt gccggaactc accgactacg cctcttacgt
    17701 cggcttggcg tcccccatgg atttcaacgg gctggtgcgc cattactacc tgcggcaggc
    17761 cgagaacctg gccgaaatcc ggctcaatct cgtcggcaag aagagccgcg ggcagcagag
    17821 ccacgccttg ggtctgcggc tgcgcgacga tttgaccgcc attgccgcgc gccacggcgc
    17881 caaactcaag attgttgaaa ccccgcccgg accgccggtg ctgtccacgc tggtggccga
    17941 aatatccggg ggcccggacc aaagctacgc ggacctgatg cgcgcagccg aggtagtggg
    18001 cgagcgcatg cggcgcgaac cgttcgtagt tgacgtggac gacgtgattg aggccgatcg
    18061 cagcaagttg ttgtttgtca cggaccagga gaaggcggcc ttgcacggcg tttcggtgcg
    18121 cgacatcgcc gacactttgc agatcgcact ggcgggccgg caggccggca cgcttgccat
    18181 gcccaatgag cggaatccac tgcgcattca actgcggctg ccgcgaccgg cgcggtccag
    18241 tatggccgac ctggggcaga tacacgtcaa gactgccggc ggcggcttgg ttccgctggc
    18301 cgaactggga caatggaaag agcaacctgt agaccagacg atatatcaca aggatctcga
    18361 acccatcgtc tacatctacg gcgaaacagc cggacgcgcc ccggctgatt gcattgtaga
    18421 tattcaggct gaccgcagac aggccgcacc gggggagtca gtcagtaggg gctgggtcgc
    18481 agacgtggct ccgcgccctg ttgacagccg gacctttcta tccaacggca gtggcattca
    18541 ctggcaagtg cccgaaggcc ttcgcgtgaa cttcgcgggc gagggtgaat ggcaggtgac
    18601 gctcgacgtg ttccgcgacc tcggactggc tttcggcgcc gcgctggtcg gcatttacat
    18661 actcctggtc gcgcagacgc gctctttcgt tattccagtc gtagtgatgc ttgccattcc
    18721 gctgaccatc gtgggcatta tgccgggatt ctggctgctc aacgcgctgg ccggcggaca
    18781 ggtgggggcc tatgccgacc cgatcttctt caccgccacc ggcatgatcg gcatgatcgc
    18841 cctggccggc attgtcaccc gcgactcaat catcctggtg gatttcatcc atctctcgct
    18901 gcgccgcggc cgctcactct tcgacgccat aatggagagc cgcgtggtgc gtctgcggcc
    18961 tattctgctg acggcgggcg cggcaatgct gggcgcagcg ccgattacga tcgatccgat
    19021 cttctccggc ctggcctggg cgctgatctt cggcctgttg tcgtctaccg tgttcacgct
    19081 gttcgtcatc ccagtggcct actggctgct ttacgctggc aagaccgggc acggcgttcc
    19141 ggaagcagag gaggacggcg gcggagacag caacgatgaa aaagaaccgg cgctgacggt
    19201 cgtgtaagtg atagggtgat aaggtgatag agtgataaag gaccacgcga ggaacccctt
    19261 tatcactcta tcaccttatc actttatcac cgtggcgccc gcggaagtgg ccgcttcgcg
    19321 cagaggggcc tacctgctcg aacgccacag ccgcgcctgc gcgatcgcct ccgccgaact
    19381 aggcgccgcg cccaccgccg ccagttcgcc cggtcgccgc gcccttacga tccgcgccag
    19441 cagcggcacc gccaaaccgc gggccagcgt caatgcaacg aacaagcccg cgtacaccac
    19501 ccaagtcgct cccgcgtagc ccagagccac aaacgcaccg aacgaagccg cggcgacggc
    19561 ggccgaattc agcaggttgt aggtcgtcat cacgctggtg cgctcgcggt cgggaatcgt
    19621 gtcgaacacc agcaggcaag tggccaattc gtacgccccc agcgccagcc ccgacagcac
    19681 ctgcacggcc accaggtacc aaaactgcgg ccacatcagc cacaaagcgg ttaccggaac
    19741 catcgccagg cccgcgacct gcagcagggc ccgcgcccca cggcgatggg ccagctcccc
    19801 aaagagcgcc agcccggcaa tcttggccag gaattcggtc cccagcagcg acacgaactg
    19861 ccagtaaggc atgtgcagat ggcgtaacac aaaggccgtg aaatacgcct gcgccacgag
    19921 catcgtgacg ttgatccaga ccatgtacgc cagcaactgg ccccgctcgt tgcgcagccg
    19981 cgtgaagaat tcgccaaacg ccacgaatcg ctcgggcgcc acgggcacga tcgattcacg
    20041 ctgcagccac aggcagcctg ccgacaccag gcggcatata ccggccaacc cgaacagcag
    20101 ggcaaacccg gcgcaggtcg ccccgcgctg ctcggtcagc tccagcaccg gccaggccat
    20161 caggatgccg cccagcgtga ccagttggaa caggcgattg cggcgggcga agtagcgcgg
    20221 gcgaatgcgc gtcggtacga ggcgcgtgat ccaactggtc cacggcgctg cacacgccag
    20281 gtccgcagcg caatagaacg tggcgatcat tagcagtgcc gcggccgaga cgtggccgcg
    20341 tagggccgcg attaccaggg gcaggaaact caggccctgc acggtcgcgc atccggccac
    20401 ccagcggcga tgtgaacgca gccagaccac cgcgtagggc gagacaagct gcaacagcgc
    20461 cccggccacc aacggcgcgg tcaccaccag cccggaaagc tgctcagaca gccccaccgc
    20521 cagcacgaag gccggcaggt agcggtgccc cagccccagc attccgccgt acgccgcgcc
    20581 gtcgactgcg ctagccagca gatcccgccg cagcccggcc gcccgcgccc gcaagcttcg
    20641 gcgccgcgtc gtagcgccct cacgcgccgc acggccgcgc ctgccgggat catgcccggc
    20701 tgggacatcg ggttcctgcc cggccagacc gtcgggtaaa tcgcttctca tgcgggtcgc
    20761 ccgcccagac gcctccatga gccagatggt aagcacgacg cataccacgc cgccagccag
    20821 gccgcgctcc cggtacgaag gccatcttcc ccgggcgccc gcgacgaacc tgcctccgaa
    20881 gcaatatcgg tcacgcgtgc cgtccgcgcc tggcctttgc cgcgccgccc gcccgcgcct
    20941 atcatgctcc gctcaaacaa gtaggagact taaatgcccg tcgacaccgc tgtcatggac
    21001 aaactcacgg ccctctgcaa gcgccgcggc ttcatttacc aatccagcga aatctacggc
    21061 ggtatcggcg gcttctggga ctacgggccg ctgggcgtcg agctcaagcg caacatcaag
    21121 aaccagtggt ggcatgacat ggtcaccaac ccgcccatcg gccccgacgg ccgcgaggtc
    21181 gaaatggtcg gcgtggactg ctccatcatc atgaacccga aggtctggga agccagcggc
    21241 cacgcgagcg ggtttgccga cccgatggtg gattgccgtg agtgcaaggc gcggtttcgt
    21301 gcggatcagg tgatcgtgct agacatctac gctggcgaag actacctgac cagtgagagc
    21361 attgaggcaa attcatcggc ggatccatct atcgaggaat atcgaaggcg agccgcgttt
    21421 gttgaagcgg tggccggagg tccggcagac cccgaactga aaaaaggagc caagaagttc
    21481 cgacaggcgc tccgcgacac cgtcatgagt aatcaaaacc tgatcgacgt ggtcgtgaga
    21541 gcctcaggcg gggccgttac ccgggatgag gttccaaacg ttctgaaatc tatcaaactc
    21601 aacctacgtc ctcgaaaact aggtgaccag ggtcctgcga acgcgagagt ggcatgccct
    21661 cgctgccatg gcgttgactc attaacggag ccgcgggcgt tcaacctcat gttccagacc
    21721 tacgtcggtg cactgcagga tgaatctgcg aaggcctacc tccgccccga gaccgcccag
    21781 ggcatcttcg ccaacttcaa gaacgtcctc gacaccgcgc gcgtcaaagt gcccttcggc
    21841 atcgcgcagg tcggcaagag cttccgcaac gagatcaatc cgcgcaacta caccttccgc
    21901 tcgcgcgagt tcgagcagat ggaaatcgag ttcttctgcc acgaagccga ctcgatgaag
    21961 tggtacgaat tctggcgcaa cgtgcgccac tcctggtaca cgaacctcgg cctgcgcagc
    22021 gacaagctgc gtctgcgcga ccagggcccc gaggagctcg cccactattc caaagcctgc
    22081 gcggacatcg agtacctgtt ccccttctcc gacgagccgc aggagcttga aggcgtcgcc
    22141 caccgcggca acttcgacct gacgcagcac gccaagttca gcggcaaaga cctgacctat
    22201 ttcgacgacg aagcctggga acgcgacaag gccaagttcg gcggcgataa ggaggccgag
    22261 aagaaagcca aaaacgagcc gccgtaccgc ttcctgccga cggtcatcga gccgtccgcc
    22321 ggcgccgacc gcgccgcgct ggccttcctc tgcgaagcct acaccgaaga ccaggcccca
    22381 gacgagaagg gcgtcatgca gacgcgcgtc gtcatgaagt tccatcccaa gctcgcccca
    22441 accaaagtgg ccgtcttccc gctcgtcaac aaggaaggca tgcccgacgt ggccgccgcg
    22501 atttaccgcg atgtcaagaa gcacatgacc gccttctacg acgaaaaggg cgcggtcggc
    22561 cgccgctacc gccgccagga cgaagtcggt acgcccttct gcgtcacggt cgacggccag
    22621 acgctgcagg acggcaccgt cacaatccgc gaccgcgaca ccctcgcgca agtccgcgtg
    22681 agcaaagacc aggtcctacc ctggctgcaa gaaaagctag gtggctgcta aaacagccag
    22741 cctggcgatg agctgctaaa acagctaccc gggcgataca gctacccggg cgatgacgtg
    22801 ataaggtgat aaagtgatag ggtgataaag ggcgtcccgc gccgcatgca gggtgccaag
    22861 ttgggtgcca tgcccaagct gtaagcgcgg gcatgcccac gtgaatacct ccacgacgca
    22921 ctcgccgctc gctgcctctg tgaagccgcc ccgcgggcgt tgcattacgc cccacgcgac
    22981 tacagcgccc gcagaaacgc cacaaggtcc ttcttttcct gctcgttgag ctgtagctgc
    23041 tgaaccaggt tgaagaactc aaccgtgtcc tccagcgtca gcgcccggcc gtcgtgcagg
    23101 tacggcggcg actcctttat cccccgtaac ggaaacgttt tgatcggacc gtccgccgtg
    23161 gcgtaccggc cgttgatcat tttcggctcg taaaaacgct cggtccgcag attgtgcatg
    23221 aggccgtccg tgtagtacgg cgccggatga cacaccgcac actgcgcctt gccgaagaac
    23281 agcgcctgcc cgcgcatctc gctctcagtc gccttctgcg gatcgagctt gccatagata
    23341 ttcagcttcg gcgccggcgg gaaatccagc aactcctgaa actcggccat ggcatgcact
    23401 tcgctgccgc gctcgaggaa gttcacaccc ttcttttggg caatgactat gtcgccgtca
    23461 aaataggccg cccgctgctc aaattctgta aagtcttcca ccgtccgcag tgcccgctgc
    23521 gaaccgaaca gccgctgaat gttcacgccg cgcagtgtcg gcgtatcaat ccgatgccgg
    23581 aacgcctgcg ggcgaatgtc gcccaccagg tgcgtggcgt tgttcgtatg cccgttggcg
    23641 tgacagtcaa agcaggtcac gccggcgtgc ggctcggcgc tgcgccggtc attagtgaag
    23701 ttgaactgct gctgcgggaa ctggctcacc agcagccgca gtccctcgag ctgcttcgga
    23761 ttgagaattc cgttgaacag ctcgaagaag ttcttctgcg tgaccagttt gccctgcgat
    23821 acatcgccca ggtccggtcg cgtggtcagg aagatggccg gcggaaactc cgccagaaag
    23881 tggtcgggaa tgtcgaagtc cagatcaaaa cgcgtcaaat catgcaggcc ctgccgcttc
    23941 aattcgtcga tctggaattg cgggaatacc atgccgcctg cttcgtggtt ggggtgcggc
    24001 aacgggtaaa aacccttcgg ccagaggtct ttttcacgga tttcgtccgg cgtcattttg
    24061 gccaggtcgt cccaggtcac accgccatgc agcttcacgc gcacgccctg ctggatgggc
    24121 ttgccgcgaa acatggtgac gtctttggcc gggttgtcgg aaaggtcgta acgctcctgc
    24181 agcaattgca tttgccgctg catgatttgc ggcttttcgg cctgcaggcg cttcatcatt
    24241 gcgtcgaagt cgatgtccag gtccaccggc atgtagctgc ttgtgccgcg ctccgcagcg
    24301 ccggcggcct gctcaccgcc gccggcagcg ggctttacct gcgcgagcag cggaagagcc
    24361 agcaatgttc cgcccagaag cgttccgacc accgaaagca cgcgggtgcg gctttctcgc
    24421 ttagcgtacg tcatgtctgc tcctttgctt acagaggcgg cgccgccgcg gaatgggacc
    24481 gttgtctata ggaatgtagt agccattacc aattcgtcaa caattggtct tcggggctct
    24541 atttagaaga gatgtgggtt cgactagtcg tattgcgact gcacccctga atgcgagagt
    24601 tcaactcgca atgttcgagg gatcgaggga tcaagggatc gagggatcaa gaggtgccgt
    24661 gtcgccgccg acagcagagc cgaggcgtgc cgccccgaaa tgccgccaag aatctcccac
    24721 tcgcccagtg gccgctgggt gccatgccca agctgtaagc gcgggcatgc cctcgctaac
    24781 ggcgccacgt gcgggcctgc gttgagttgc tggcgacggc atgcccgctt ccacaacacg
    24841 ggcttgggca tggcacccgc cgtgaatgct ggatgccatg cccaagcgcc aagtgtctgc
    24901 taaccttttt gccttaccgc cttgctgtcc gggcggtgag cgtccgctta aacgaccgcc
    24961 ggatcagcgc gcggcgccga caggctccgg cttcgtggtt tccgtttcca tcgcagccat
    25021 ctccggcgcc tgccgcttcg actgccaaac ggcccagatg atcagaacca gcgaaaccag
    25081 gacggcgccg atccacacgc cgtcgatgct cttgggctgg aaggactccg gtagcgtaaa
    25141 cgcgaattcc ttgcctgctt ccagagcctt ctgagcggcc tgtgcggcgc cctcgcgaac
    25201 cgtccgcacg accccggcgt catatgcgac catcagcggc acgcccagga tcgccaccat
    25261 gttcatgacc ttcagcagcg ggttcagcgc cgggccggcc gtgtccttca gcgggtcgcc
    25321 cacggtgtcg cccgtcacgc tggctttgtg cttctcgctg cccttgccgg tgttgcgctc
    25381 caggtcacgc ggctcgtctt cgaccatctt cttggcgttg tcccaggcgc cgccggcatt
    25441 ggacataaac gacgccatga gctgcccgac cacgatcgca ccgccgagga agccggccag
    25501 gccgatcgga cccagcagga agccgatcaa aatgggcgtg aacacgccta gcaaggccgg
    25561 accaatcagt tcgtgctgag cggccgcggt gcaaatcttg accacgcggc cgtagttcgg
    25621 cttcttcgtg ccttcccaga tggccttgtc gcggaactgc acgcgtactt cattgacgat
    25681 cagatacgcc gcgcggccga ccgcacggat caacataccc gagaagagca gcgggaccgc
    25741 accgccaatc aacatgccga ccagcagttt cggattcgag accgtcaaca gggccgccac
    25801 atggttgaac agcgcgatcg gcagcggctt gtcctcgctc tgcccgccgg agccgatgac
    25861 cgtgatgaac gaggcataca gcgagacggc cgcaatcacg gccgagccga tggcgacgcc
    25921 cttcgtgacc gccttggtcg tgtttccgac ggcgtcgagg tccatcagga tctgccgcgc
    25981 ttccttgtat ttggcggcgc ccatctgctc ggggtcgtag cccatctcgg cgataccgtt
    26041 ggcgttgtcg gcgaccgggc cgaacacgtc catcgaaatc gtgttgccgg tcagcgtcag
    26101 catgccgatg ccgcacatgg ccacgccgaa cgcgatgaag atcgcgttag tgccggcgta
    26161 cacaaggacc gagaggaaga tcgcgatgca cagcaccagg gcggtccaga ccgcggactc
    26221 atagccgacc gcgaagcctt cgatgatggt ggtcgcgtgg ccggtcgcgc agttgcgctt
    26281 gataccgcga acggggctgt actcagtccc ggtgaagtac tcggtggtgc ggttaagggc
    26341 gatgcacaag agaatgccga tgaagcaggt gatcgccggc cgcatgtcga ggccttcgat
    26401 accccagttg aagtaccacg gcagggtgct gatgctgttc caagccacgt cggagagtgt
    26461 tcccgccgcg accatgtcag cgttgaaccg gaggtagaag aagcccagga tgatgaaccc
    26521 ggtcaccgat atcagcgagc cgagcacgaa gcccttgttg atcgacctca tggcctcggc
    26581 cacgtcgccg cggtcgccgg cccttacgga gtacgtcgaa atgatcgagc cgatcacgcc
    26641 gatggcgcgg accagaatcg ggaagattac gcctttgtga ccgaacgaag cccatcccag
    26701 aatcatggcc gccacgatgg tgacctcgta cgactcgaaa atgtcggcgg ccataccggc
    26761 gcagtcgccg acgttgtcgc ccacgttgtc agcaatggtg gccgcgttgc gcgggtcgtc
    26821 ctcgggaatg tctttttcga ccttaccgac caggtcggcg ccgacgtcag cggccttggt
    26881 gtagatgccg ccgccgacac gcatgaacaa ggccagcagg gtgccgccga agccgaagcc
    26941 cagcagggcc tcgtaggccc gctcgccgta gaccataaag atcagcgttc caccgagcag
    27001 cccgaggccg tcggtgagca tgccggtaat ggtccccgtg cggtagccga gcatgagggc
    27061 ccggccgaag cccaccggag cggccgcggc cacgcgcagg ttgccggtcg tggccaggcg
    27121 cattcccaca aacccaaccg tggcggagaa gagagcgccc atgaagaatg ctccggcccg
    27181 tccgaaagca aaggggctca tccagtcgtg gtcttggaca gctttggtta ggtacaacag
    27241 gccgatcaga atgagaatca tgacggcgac cgtgctgagc tgccgcttca agtaggcgtt
    27301 ggcgccttcg cgtacggcgt cagcgatgtc ctgcatgcgt ttggtgccgg tgtccgcgcg
    27361 gcggacgaaa ttgaccaggc tataggcgta cacgagcgcg ccgatcgcaa cgaccagggc
    27421 cccgaccagc gcgatttttt cgcccagcgc gaagtcgggg ttgcgcatta cctcccacca
    27481 ttggaagtgg tgctcccccg catgcgcggc tgtttcggcg gccggcgggg cctgagccag
    27541 caggccctgc ggcgtgccgt aaatgctggc gtgggcgtag aggcccatga agagtaaggc
    27601 gaaaaaagcc aggaaagtct tgggatatct tcgaaagcac gatgcaataa gcgaacgact
    27661 acgtctcacg atgagctccc gccaagaaac ctaaaacttt agtgccgagt ctcgggcgac
    27721 ggcccgaaac aggtgagcat tgcgccttga tgcgcgaacc gcaggacgcc atgctccgag
    27781 attacggagc cgccactata gggcttccgt acagggcttc aagggtccgc tgggggcaaa
    27841 tccgggagcg ggtgggcggc tgcgcggggg gggtacggct gggttgcgct caccctgaag
    27901 cccggccgcg ccgcccagcg cccccgctcg aaaggcgggg cccgccggcc ggagcgccgg
    27961 caacggcagc cgcgccgcgc tcactcttcc cgcttcgggc tgattcacct acaatctgag
    28021 atctggacga cctacaacgc ggaggcatta tgagcggaca ttctcactgg gcacggatca
    28081 agcacgccaa ggcggtcacc gattcccgcc gtggccggct gtggagcaag ctgtcgaggg
    28141 caataatcgt ggccgcgcgg catgggggcg gcgatcccgc catgaacctg acgctgcgct
    28201 atgcgataga cgccgcacgc gatgcaaata tgcccaagga cacgatcgaa cgagccatca
    28261 aaaagggcac cggcgcgggc ggcgaggccg attacgtcga actggttttc gaaggctacg
    28321 gcccaggcgg caccgccatc ctgtgtaccg cgctgaccga caaccgcaat cgcaccgcac
    28381 ccgaggtgaa gaaaatcttc gagggccgcg gagggaagct cggaacggcc ggctcggtgt
    28441 cgcgcatgtt caaccagcgt ggggtcttca cgatcgcggc gaccgcggcc gacgaggaca
    28501 cgctcaccaa catcgccttg gaaacggggg ctgatgacgt gcaggcggtc ggggatgagg
    28561 ccttcgagct gacctgtgat cccgccgttt tcggcgaggt tcgcaaggcg ctcgataacg
    28621 ccaacatcaa gcccgaaagc gccgccattt cgatggttcc gatcacgacc gtcatgcttt
    28681 ccggcgatca agtcgaaaag atgatgaacc tcattgaggc gctcgaagaa cacgacgacg
    28741 tgcagaacgt ctacacgaac ttcgagatct ccgacgacga cgccaaacgc ctggcagagg
    28801 cttagcgcgc gcggtcgctg cccgatcaac gccgattagc ccgtgggact tcggttcagc
    28861 acgcggtgaa aatgtcggcg actcttgccc gacttaccct cgccagggtg atactacgtt
    28921 cgcttgcagc aattctgccg gcagcggcgc ccgccgctgg tgccgaaatt ccctgacggt
    28981 agcgccaaag ctcgccaact gcggagcttc gcagcacggc cattcggccg gccgccgatg
    29041 cctgcgccga gccccctgcc ctcaacgcaa aaggcgccgg ccagttgtaa acttttggcg
    29101 ttgccgtcaa aatggcctcg acattctggc agactcgagg aaggagcaat catgccgcga
    29161 tggagcgcac acgtcctgtt ggggttcttg tttggcgccc tgttctcatg caacgaaccg
    29221 accgcgaacc tggagccttt taaagcgccg gtgggcaagg actacagccg gcctctgccg
    29281 cctggcgctc tagcgctgcg caaaatcggg cccgagcaat acccggattt ctcgaaaggc
    29341 tatcacaatc tcgccggcct cgagcaggcc attcagcaca gcctcaacta tctgagcaag
    29401 ccgtccagcc agaagtactt cccctacggt gacatcagcc acgcccgcgc ggttgcttcg
    29461 ctcaatcact tcctgcaggt tctgcactcg gtgcgttccg gggcggagct cgatgccgcc
    29521 attcgtcgcg actttgacgt ttaccagtcc gttggctgcg acgaccaagg caccgtctat
    29581 tacaccggct actacacgcc catcttcgaa ggacgccgcc aaccggacgc tcgatttcgc
    29641 tatccgctct acggcctgcc gccggatctg gtcaagggtg acgagggcgt cacgctgggc
    29701 cggaggacgc cggacgggcg cattgttccc tactacacgc ggcggcagat cacggaaggc
    29761 ggcctgctgg atgggacgga agtggcatgg ctgaaagatc cgtttgaagc ttacgtcgtg
    29821 actgtgcaag ggtcggcgaa gctgcgcctg gccgacggga gcctttacga actggggtac
    29881 gcgggtaaca acggacacga gtacacgccg atcgcgctgc aaatggttaa tgacggagtt
    29941 ataggacgca acgaattatc gctccaaacc atgctccgat atttctccgc caatcccgac
    30001 aaggtgaatt actactgcct ccagaatgac cggtttgtct tcttcaaaga actgcccggc
    30061 gggccctttg gcagtctgaa cgtcccggtt acgccatatc ggagtattgc gaccgataaa
    30121 gaggtattcc cgcgcgcctg cttggcgttc ttaaacacaa cgctgccaac gacttatggc
    30181 ggctccctgc agattggacc gtactcagga tttgctctgg atcaggatac cggtggggcc
    30241 attcgcgctg ccggacgttg cgatgtatac atgggtatcg gaccacaggc cgaagcgacc
    30301 gccggccgca ctggagctga aggcgctctg tactacatat tcgtcaaaga cgggtccggc
    30361 acgttgtagc aataattcag ttgacgccgg cgagccgacg atgcataata aatgagagac
    30421 ggagtcatcc ataagctctt gtccggaatg gtgaaggaga gctgacctaa aacatcataa
    30481 acagcgttcg gaaagccgcc cgtttactga ttatccaagt tccaaaatca tctatccata
    30541 cctgggttat tgcggctgga aggacctgtg gggcgaagct gcgccccgag gtgccccatg
    30601 caatcgctcc aggcccccgc cctcgcggag cttgccgttc agttgactcg cggtccgagg
    30661 cgtctacgcc tacggcaact cctcaacatt gaattcctcc tctcagttat cgacccgggg
    30721 aaggcatacc cagcagattt catctgccat tgccttaccg ggtatcggcg cgtcccgccc
    30781 gatgggaccg ctgacgagag gctcatcgaa gctgacgtcc taatagcaga tttggtagct
    30841 ctcgctgaac ggctcagcga ggacgccaac atcgcggccg aggattggcc gaccgcggtc
    30901 tactcgattg ccgagctggc tgaacgtttc gacgtcagca ccaagacgat cttccgttgg
    30961 cgccgccgcg ggttggtcgg ctggaagttc cgctacgccg atcggcgtat gcgggtggtc
    31021 ttcccggatg cttgcgtgcg gcggttcgtc gcgcagcatg ccgaaatcgt cagccgcggc
    31081 agcagctttt cgcaactgac gcgcgccgaa cgcgacagca tcgtcgagcg cgccagggcc
    31141 ctcgccggcc agggccagag cacggtcaat tccgtggctc gcgccttggc ggccgaaacg
    31201 ggccgggccg tcgagacgat ccggctgatc ctcaaagccc acgacgagag ccacccggga
    31261 gcgggcatct tcaaccgctc acggctggac gtggcggcgg acgatcaacg cctgcgcatt
    31321 tgggaagccc accaggatgg cgtttcggtg gccgtgctgg cggagcggtt caatcagccg
    31381 ccatcagcga tctaccaaac gattacgcag atgcgggcgc gggatatgaa gtcccgcaag
    31441 atcgagtaca tcgccagccc ggcgttcgag gagccgggga tcgatgaact catcgccaac
    31501 gatccggacg tgcaggcacc gtatcgcccc gacaccggcg ggccgcggcg catcccggcc
    31561 gacctgccgc cgtatttgca gcagcttttc cgtatcccgc tgctcaccac tgcgggcgag
    31621 gcggccctgt tccggaagat gaactacttg aagttccggg ccgaccggtt gcgtcagcaa
    31681 ctcgacccgg atgaggccac ggccagcgac ttggacgcga ttgacgcgct gctcgacgag
    31741 gccaacgcca tcaagaacca gatcacgcag gccaacctgc gcctggtcgt gagcatcgcc
    31801 aagcgccacc ttgggccggc gctggacttc ttcgagatca tcagcgacgg gaacgtctcg
    31861 ctgatgcggg cggtcgacaa gttcgactac acgcgcggct tcaagttcag cacttacgcc
    31921 tcgtgggcca tcatccgcaa ctacgcccgc ttgataccgg agcagcgctg ccagcgcgac
    31981 cgttaccaga ccgggcgcga agagctgctc gaaatcacgg ccgagcgcgg cgcggacgag
    32041 caggatgacg agcagaatgc cctggtccgc ggcgtcgtgg aacgcatgct ggcctcgctg
    32101 gatttgcgcg agcgccgcat cttgcggcag cggttcgggc tggacgaaca gcaaacgccg
    32161 cagacgctcg agcagatcgg ccggcacttt ggcgtgtcca aggaacgcat tcgccaactg
    32221 gagacgcgcg ctatccgcag gctgcgcacg gactttgcgt tagacgtgca gcgcctgctc
    32281 ggtagctgaa gcctcgcagc gggggccagt gccggcggcc cccgcttgcg gtttcactca
    32341 gtcgccccgt cggcactgga tcgccagcgc cgccccccgc gcatttctga cgatgccggc
    32401 ggctctgtcc tcgcccggag ggcgcacgag cggtagccag gggcttcagc ccctggaaaa
    32461 ccgaaacctc accccttcct cccgcccggc agggcgggcg aacccttcat acccgccagc
    32521 ttttcttcct acaccccatc gcgacgccag gccgaccgcg cccggcacgc ccgattacgc
    32581 cgctcagcaa ctacccgcca tccgtgcgtc ttttgccgct tcgttgacct tcaggcattg
    32641 agtcgtgata cctttccctg acatcagcag cgcgatcgtt ccgcgagagt acgctggaga
    32701 tcgaggccga gacatcgcca taatccgcct ctaacgcgca cgcagggcac cgcccgctgg
    32761 tggcgtcagc gaacctgcca tcatacgcat caccgcagta acgacacgtc gcgctttgat
    32821 cgcactccga ttcttgctcc gccatgttct tgaggaattg aaccaattcc tcggcgcttc
    32881 cgaacgtctc ctcccggagc agactggcca aaacaagtat ccccatggcc ggattgccca
    32941 agcgctgccc aaacatgctc atgtcatcca ggactatcgg gtctatgaag atgcgtcgtt
    33001 ttcgcaccgg ccgctcaccg tcagtgagag cagattcgac ccgggtagga agatcgcgga
    33061 agatcacctt gacttcctca aacatttttg caatcgcggt gttatccgcc tcagcatgct
    33121 gctctttttg atcctgttgt ccgcgtttcg acagcagctc agtagcctta tccttaaata
    33181 tttgcacctg cataagaaag gcatcgtgat ctggctccga gccggggatg cgctggacaa
    33241 gctgcatgac gagcttggtc agggattccg gatcgtcgtc gcagttctga aactgggcga
    33301 agggaccggt gctggctttg ctcagtggaa ttccaagggc gacgccgtag accggtgtat
    33361 ccaacctgcc tttcgctacg ccggcctcat acagaagcca aggtctctct aggctgcgcg
    33421 gcgtcagcag acagactacg tctgatgctt tgtcgagttg tctctttcgc cgccattgcc
    33481 gcgcatacac ccacaatccg cgcactccgg ctcaaggctg atagtgagcc ctgaacaggc
    33541 tttctagaat atccagcacg aaccgcgccc acgcgcagaa agggcccgct atgcagcatt
    33601 cgaaacgtcc ccactgctca cctgtcacct atcaactgtc tccccgctgc gaccaaaagc
    33661 gaacttttac aggaccaggc acgcaccggt tcggccaccc ctttatcacc ttatcaccct
    33721 cgcggcgtca cctgctcgcc tctcacctat ctcctctctc cgcgcagcgg cgcggcgcct
    33781 caggttatca aaattgggtg cacataaacc acgcgcatct gattacctgc ccccaggacc
    33841 cgatcctggc gagcttttta tcagaccgct tgagcacctg aggcagccgc tccgcctgag
    33901 ccgaacgaat gttgcgctcg actgtctcat ccggcgctag gaataacgct ggacgaattc
    33961 gcgttgctgc aattgcgcca gcacggataa gattatgctt accaacgcac gaaccgtgtc
    34021 cgttaagcga accgcactgg gccgtcgggc gtccggcggt cggtgcggca aacgcggagc
    34081 ctggaggatc gtgggaaagc ggtgccctcg acagtccttt tcgacagtcc ttctcgacag
    34141 tcctcaatct ccatcggaat tgggccgtat tcccaaacat aggaggcggt ttatgttctc
    34201 gaaggccctc tcggctgtag cggcgacgct ctttctcgta ccgcagatcg cgaacgggca
    34261 aagtgccccg ctaagtgatg gcgtctacac gcggcccgag agcgcggccg tggcggtcga
    34321 aatccctgaa tcgcggcttc tggccggcgt gcgcgcctat ccgccgggcg tgctgccggc
    34381 gctgttcacc gtgtcgcagc agcccgccgt catacagcag ttggccgagg acccgcagaa
    34441 gctgatgaac ccccaggcga tcagcccgcc ggtctcggcg gagttgcaga acgcgatccg
    34501 gcagcttcag caggtccccg aggcggtggc gctggcagcc gcctacccgg acgaaatcga
    34561 gatattgcgg cagctctacg agcgcgcccc gcagcagacg gaacaacggc tggcagacct
    34621 gcgtgcgcgc tacgacgcgg cgcgcgtgca ggctggccgc gcttggcagg aacttcttta
    34681 tgaaaacccc ggggccctcg atgcctatcg cgcgctcgtg acgcagttca cgcgcgacca
    34741 attgaaacaa tatggcgact ttccttacgt cgaagtcaaa cagacacggt actactacgc
    34801 cgcgccgccg aacgaactga tcatggccta tgccgaggat cgcaagcccg gcgcgccgct
    34861 cgacgccatc ctgcagattt ggtggaaccg gtacggaccg gaccaggtgg acgacctcgt
    34921 cttttcgcag agcgacctgg ctgtcggcgg ttcgtccgtg gccgccatgt caccggagcg
    34981 gcgggccgag atgtggcaac cggccagaga ccgcacgggc tcgggaaacg cactgctgcc
    35041 aatcatcatg caaccgttgc ccgatcagcc ggccgacgcg caagccgcat tcgccatcgt
    35101 ggagaacgcc cggctgtggt ccgggccgca gaacgatcgg ctgacacaac agccgccggt
    35161 cgcgtcccct tcgccggaca tgccaggacc gaacgcggcg ccgcagtcca cgggcccgca
    35221 gtattcgcag ccgcaatacc cggcgccaca gtacccggcg ccggaagcgg gcgagtccgt
    35281 tctgcccgag gaaccgccgg cctatgtcga gcaaggcccg gattacgaat acgaatacga
    35341 gtacacaacc gaagttgggg tgcagccgca gatcgtttac acggctcccg caaccgtcta
    35401 ctacagcccg ctcgtgccct actactacgc ttatccggcc gcgtggccgg tggcctacag
    35461 ttgctatccc ggcctgtaca cgagctggcc atacgactgg tatgccccgg gcgcctattt
    35521 ctccttcagt ttcggccgcg tgcctcacta ttaccgttgt gggtcggcct actggggtcg
    35581 cggctattgg ggctgcggca tttggcgcgg gcacggtcat tgggcacgcc cgcaccatta
    35641 ctacggtcag ggataccacg gtcgcgacgg gtaccacgtt ggtcgttatg gccgtgggct
    35701 ggatcggatt acccatcagt tccgcggcgg cgagcggtac gggcacggaa gagatcggat
    35761 cagtcctatt ggccgggatg cccgccgcgg ggatcgctac ggtcttgccg accgcattgg
    35821 cgcgttgcgc agcgatcgct tgggcaccgc gcgcggcgat cgctcgggca ttgtgcgcgg
    35881 cgatcgctcg gcaagcgcac acggcgaccg cttgggcagc gtgcgccgtt cggcaaccga
    35941 tcgcggtgag cgaatcacgg gcaaggaccg ttcggcagat ggccgcagcg aacggctcgg
    36001 cggcgatcgg tcggcggcga gtcgcggcgg agccgaccag cgctccggca tacgcggtcc
    36061 ggatcgcaag gttgaccgcc gttcgccgga aggcagtcaa atcttgcgtc aaggcagtgg
    36121 cgggcggtcg ctggagcgag gtcagagcac tcagcaacgt ggcggcaccc ggtcgcgcga
    36181 cgcaattccg ggctccgtaa gtggtggtag ccgatcgtcg aaaggagcgg caggcgtacg
    36241 tcagggcgct ggacgccaga gtacccaacg cggacaggcg ttgggcgggt cgtcaccgtc
    36301 ggcaggctcc agggggacgg gttcgatcaa tcgcacgccg ggcgctggcc ggtcgtccgg
    36361 agtgtcccgc ggaactcaat ccgctccgcg cagtatcgcg gccccccgca gcccctcgac
    36421 ttcgcgcagc cccgcggccc cgcgcggttc ctcgacttcg cgcagccccg cggccccgcg
    36481 cggttcctcg acttcgcgca gcctcgcggc cccgcgcagt tcgggcggca gtcgcccgat
    36541 gggaacgatt cgcagtggcg gcggcatgcg gagcggcggc gccgggctgc acagcggaag
    36601 cggagtccgc agcggcgcca gcgggcggca aggcgctggc agcgggctcc gtagcggcgg
    36661 cggattgcgc agcggcggac gccgctagaa gcgccgcctc tactgttgcc gagaaccaag
    36721 cgccgggtgc ctcgcccaag ccgggtgcca tgcccaagct ctaagcgcgg gcatgcccac
    36781 gtcgaacgcc gccaagtgta agtttattga cgacctcatg cccgcgctgc tgcgcggctt
    36841 gggcgatggg tgccactgct tgaaagcgca gcttcaagca gtgctgcccg ggtctcgtta
    36901 gagccctccg cgggcagcaa gattagcaaa ccaacgcacc aaagcttatc atgccatagg
    36961 aacggcatag gggcccacgg tccggaagga gacaagtatg ctcgctctaa tttggctgat
    37021 tatcgcaggc aatttcgtgg cgttcgcgga cgcgccgccg ccggccgagc ggcagttgcg
    37081 gggcgccgtg gatacgcgcg tggaattgat gagcatcatc ttccgcctgg ccggcaaccc
    37141 cgaatacaac atgcccaact cgaactcgcg ctacgctgac gaagttgagc agcacttcgg
    37201 ccgcctgcgc aaccacccgg ccgtgaagta cgcccaggag ctccgccaga aggccggcat
    37261 cagctacgac gccgtgatga gcctggccgt acacaccacc gatgcgctag agttgaacga
    37321 gaaaatcccc ttcgaccagg ccccggcgcg cctggacgcg cgctggaaga ccgctgaggc
    37381 gcgcgagttc ctgaagctcg cgcggcagtt cgcgcgtgac agcggcttcc ggagattcgt
    37441 ccgcaaccac gaaagttact acaacgccgt ggccgagcga atcaatgagc agctcgcgca
    37501 gcgcgactat ctgaagtggt tcgacgactt cttcggagcc caaccccagg ccagttactc
    37561 ggtagccgtc gggttgttga acggcggatc gaattacggc gtcggcgtgc gttatcccga
    37621 cggccgcgag gagataacgc cggtgttggg cgtcgcgcag ttcgatgagc agggtctgcc
    37681 ggtgttcgat gacagcttcg gcccactgct gatacacgaa ttgtgccatt cgtacgccaa
    37741 cccgcacgcc gagaagtacg agaagttgct cgagccggcc ggcctcgctc tgtatgcccg
    37801 agtgggcgaa cagatgaaga agcaagccta cagcaactgg aaaaccatgc tgagcgaatc
    37861 cctggtgcgg gcctgcgtcg cgcgctacca actggcggcc gaaggcgagg aagcggcggc
    37921 caagctgcag gcgcaggacg ctgcgcgcgg cttctattgg attggcgacc tggtcgaact
    37981 cttgggccag tatgaaaaca cgcgccagga gcacccgaag ctggacgact tcatgcccga
    38041 tgtg
//
LOCUS       DAOSYS010000005        36128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_946_length_36128_cov_5.887423, whole genome shotgun sequence.
ACCESSION   DAOSYS010000005 DAOSYS010000000
VERSION     DAOSYS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 36128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36128
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_946_length_36128_cov_5.887423"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="PLU99_00645"
     CDS             <1..1852
                     /locus_tag="PLU99_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15589.1"
                     /translation="YLGFTTWLGAKMAGRQATIRDFFLGGRKLPWPAVSGSIIATEIS
                     ALTFVSVPWVVFKPGGNLTYLQLGVFGSLLARILVGYILVPAYYKREIYSPYDYMHNQ
                     LGGHVRCLTTGLFMLGGLLGQSARIYLTAEVINVVLHDQLVALSQGLGLNELAWAIIL
                     ITGVSVAWTLIGGMSTVIWTDVVLFLAFLLGAITALAVVVASLDGGLLEVIRTGWAAK
                     ESGPWGKFTFFDFSPSPVRSYTIWTAVIASTWGGLGSYGTDQLMAQRMFCCRDYRAAR
                     RALIASSTSQIVTFIVAFVGIGLYAYYQSHPLQGEALDLFRAKGDRIFPIFTVEVVPR
                     GLKGLIIAAIFAAAISSVMGILTALSQTAQTAFYEPLMRTLRRRKQRSAGAPLYSRQP
                     LAGSRIGGATTVAVAAESSTRHGDDTTEEPGHNQGRASVMASRGLVVFWGILLGLLAY
                     WSQQVASRYPSILDMGLAMAGYVSGALLAGFLLSFLPLRVDSRGFIHAAPLSCMCVFA
                     LIWHAEWSNVVCWVAAGVIFAGWLLQLYLDFLSKRGERARTVLPAPLQTLVLLAGLAF
                     MLWLNYRGYWPDPTGGTKHQTVAWPWMIPLGSTVAFVWGYLLARFRPCPE"
     gene            2023..2190
                     /locus_tag="PLU99_00650"
     CDS             2023..2190
                     /locus_tag="PLU99_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009806310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOO15590.1"
                     /translation="MLYWAIVFFVIAIIAAIFGFTGIASGAATIAKILFFLFVVLFVL
                     ALLAGLLRGRT"
     gene            2417..4078
                     /locus_tag="PLU99_00655"
     CDS             2417..4078
                     /locus_tag="PLU99_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15591.1"
                     /translation="MKKFWQPLVTITIGASCLLALGLAGCNSANKCNSETTCAPRAAY
                     LAECPRCAVCEHRSSPCSACTASWRSRCERETCSSIYLPTGDRSDAVLKLEKCGPREV
                     RAGEPFKYTIKVTNRTKHALRNVTVIDELPQDVSVQGPIGGGAGWLPHDRPAGAHAAN
                     VVRYPVGDLGPRESRCIEQCGVAGGTGCLSSCLTARYDLFACLQTAVTQPQLALCMTG
                     PQDVFICRSDQDANLHVTVRNTGSATADNVRVTVSIMPPAGAPQLAAAADSERMVGAL
                     GCNEERSFDICLPVLAPGEYRVAAQAVGDAGLRTERSELLFFARFAALNIRAAGPDQE
                     LIGLPIEYRILIENTGNAPDTNVTVTAELPPGVLFLEATDGGQPAGERVVWNIGNFPP
                     GQPRELGLRVRATDVARTINPVFVVCGDCGGQQSVMLATALEGVPALLLEMIDIQDPL
                     RIGELVEYRVTVINQGSAPETNIRMAARLPDELTFVRSTGPTEPRVEGTELTFATVPL
                     LPPKEKITWTIFAHCDRPGDMRMAVQVQSDQAQRPIRETEATRVY"
     gene            4312..5202
                     /gene="mtnP"
                     /locus_tag="PLU99_00660"
     CDS             4312..5202
                     /gene="mtnP"
                     /locus_tag="PLU99_00660"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOO15592.1"
                     /translation="MLGIIGGTGLADALFGQVDGADHVIDTPFGAPSGPVRVVHWQGL
                     EVALLARHGAGHLINPSQVPYRANIYAMKSLGVTHLIASGAVGSLREEFRPRELVIVD
                     QVIDRTYRRVPTFFDAGLAAHVEFAEPFCPVLRQRLLAAATQIETKVHPHGTYICMEG
                     PAFSSQAESRMHRAWGGDLIGMTLMPEAKLAREAEIAYAAIALITDYDCWRPHAPGAT
                     KQKLLEEILDNLHAATHNAIALLRAALADFARQPPGASPAHSALELAIWSDRAKIAPE
                     TVRQYGVLLKRQLGEQKQNG"
     gene            5306..7069
                     /gene="recJ"
                     /locus_tag="PLU99_00665"
     CDS             5306..7069
                     /gene="recJ"
                     /locus_tag="PLU99_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOO15593.1"
                     /translation="MEAFRRPRFRGECEWIIAQPHPDRDRLASQAGIAPLLAQVLLNR
                     DISTAAQVGRFLSPDLGLLLPPEALPNAVSAGRRLAEAVRAGRRIVIYGDYDVDGITA
                     TSILWLALRLANANVSFYIPNRLEEGYGVNGEALRSIAAEGDALVITVDSGITAVAEA
                     RLARQLGLGLIITDHHEPGPTLPECDCVVHPTACGASENPDLSGAGVALKIAWAFAQE
                     FCGTQRVSEQFRNFLLDATTFAALGLVADVVPLTGENRVIAAFGLKYLRRTQNPGLKA
                     LLQVSGLEGKSRYDDYDVGFMLAPRLNAVGRLGHARTAVELFTQADSARAAEIAAALD
                     AQNRKRQELERTIAKQAEALVIERGFDRDGCHGIVLACEQWHAGVIGIVASRLVNRFR
                     RPAVLIALENGCGQGSARSVPHFPLHEVLACCGEHLLSHGGHAMAAGVRVAADRVDAF
                     TEAFLAEAARRLTPKDLCPRLYLDDEVELTDLTIDVVDALQRMAPHGTGNPAPRLATG
                     VVELAEPPRVVGKAGNHLQMMVRQGREHRKAIAFNRGDQAEQVNEVRRLRLAFEPIIN
                     EWNGRRSVELKVVDWKFGDVR"
     gene            7296..7466
                     /locus_tag="PLU99_00670"
                     /pseudo
     CDS             7296..7466
                     /locus_tag="PLU99_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010602585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1"
     gene            7600..8073
                     /locus_tag="PLU99_00675"
     CDS             7600..8073
                     /locus_tag="PLU99_00675"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HOO15594.1"
                     /translation="MPPPAILDPLSLDFSRPFATREQIAEVNPHRHEFALLDAVVTFD
                     REAGTFSGYHDVRPDEWWARGHIPGRPLFPGVLMIEVAAQLASFLGHLVNGRDFFMGL
                     TGVDDVKYRGTVEPPCRFVVVGRALDARKRRTKCATQGFVDGTMVFEAVITGMAL"
     gene            8176..9441
                     /locus_tag="PLU99_00680"
     CDS             8176..9441
                     /locus_tag="PLU99_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15595.1"
                     /translation="MGTQRSPHPSFRDKNSPFAMSDNRSVSSEVLAALPEAVTWSHRP
                     QESDLEDVPGCPAVYLLLATSDLPVQLATTQHLRRILHSRLTDAARVQQGKADLAEVV
                     RGVRWRRMHSPFEARWWYYRLAREMYPKRYRRMISFGPAFFLRVDWSEPIPDINVTKQ
                     VWRISGEMVGPWLTHDTCQKALEGLRDLFDLCRYPEQVQRAPRGQRCLYAEMGRCDAP
                     CDGSVPLERYIERLRAAWAFARGEVRPWLAEAKERMTSAARAQEYERAALLKRQIAWG
                     WKWHDQILPRVHPLEQLNYFLAIPAHRRRACRLFLFRSGNLVEGPVLSDRNAPSEALP
                     WLANVLANPPAPADPNVRMEQTWLVAHFLSSRAGEASFRSLLPKGEPTGDLEEQLRQY
                     LVQLRTKPGDEGEHAAVRPPSSDNENQTK"
     gene            complement(9784..11109)
                     /locus_tag="PLU99_00685"
     CDS             complement(9784..11109)
                     /locus_tag="PLU99_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOO15596.1"
                     /translation="MKLSEELARKIEAHEAIVGVIGLGYVGLPLVREFCNAGFKVIGF
                     DIDPKKVSMLKAGKSYIEHLPAEIFNPFIKDKRFSPTADMKLLSKPDAILICVPTPLD
                     KMREPDMTFIRSTSEDIARRLRPGQLVILESTTYPGTTREVVQPLLDATGLRLDRDYF
                     LAFSPEREDPGRKDYTTNNIPKVVGGATPASGKLAATLYGQALTTVVPVSSCEVAEAC
                     KILENVYRSVNIALVNELKLIFERMGIDVWEVIEAARTKPFGYQAFYPGPGLGGHCIP
                     IDPLYLSWKARQYGRPTRFIELAAEINTFMPEYVVMRVSEALNERGRAVKGARILVLG
                     LAYKKDVDDVRESPSVTLIELLRARGAIVDYHDPHIPSAKPMREHNITDMRSVPLTRA
                     NLKKYDCVLISTDHSSVDYDMVVEHSRLVVDTRNATGNKKRPAGRVVKA"
     gene            complement(11209..12054)
                     /locus_tag="PLU99_00690"
     CDS             complement(11209..12054)
                     /locus_tag="PLU99_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15597.1"
                     /translation="MILLESGPARTDRNWWLGVGLVFLVMGGYFIYDGAVGYRKQNIA
                     KARERLSLWTAGQPELGETPGPEEYHLLRTRKVASRQELHELLGQPLAPREGAATAGV
                     ERFASVYGVITVPIDPSGRVRSELAEWQAWKWSKEEIRNQYYWGVIPCVLAVYFLIRA
                     FRAGRLRAEIDEQGMTYGGVRIALADIASIRDYNKKGWVDLYYRRGGQEKRLRIDNQK
                     IAKFDEIVELLCQQKKFPNPIKAYFEAQQGEDDEDKATADFGGAADAQACFKAESEGG
                     AGAET"
     gene            12232..13863
                     /gene="gpmI"
                     /locus_tag="PLU99_00695"
     CDS             12232..13863
                     /gene="gpmI"
                     /locus_tag="PLU99_00695"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOO15598.1"
                     /translation="MEVCHVGATVKHRPCVVIIRDGWGRNPDPNLDYANAVRLADPPV
                     DRMLNEQWPTTLIATSGSDVGLPPNTMGNSEVGHQNIGAGRVVDQESVRISKAIQDGS
                     FFGNPELVAAVERCLARRSKLHIMGLVSDEGVHSRLEHLFACVELAARRGLKEAYIHC
                     FTDGRDSPPTSGIGYIERVEQELNRIGVGQIASVCGRYWAMDRDNRWDRVRKAYRMLT
                     QGEGRAAPSARVALQRYYDDPLEPNMQGDEFVVPTVISEDGRLPLATVGSGDAVIFFN
                     FRGDRPRELTKAFTLDKFPYRAKDKAGVEREMGFPRPQKLDLFFVTMTSYEADLPVKV
                     AFPKLPRMENIAGEYLSRLGLRQFRCAETEKYAHVTFFFNDYREEPFAGEERQLVPSP
                     TVATYDQQPEMSAPQVADVVVRRIESRLDDFVLVNFANPDMVGHTGSLPAAVKACKVV
                     DECVGRVLETVKRLGGCAIITSDHGNFEQMVDPQTGGPHTAHTLYPVPLHVYGAGFEG
                     AELRDDGRLADVMPTALSMMGLDIPPEMTGRSLIL"
     gene            14153..14818
                     /locus_tag="PLU99_00700"
     CDS             14153..14818
                     /locus_tag="PLU99_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOO15599.1"
                     /translation="MPHRRVADVFVDVCTQRDYLSSDGARPTANARTVQQNVKHLMAF
                     ARWAKIPTISCVDARRPDTVRGVPHPHCVLGTPGQRKITFSVLPDHVTIESDNLLCIS
                     LEILQQHQQAILIKQHRDPFTNPKLDRLLTEVPTQHFTVFGVALEASIRLLVLGLLLR
                     HRHATVIHDACGFWNPSEADMALRQLAAKGCEIRSTHEVIRERLTQLRTGARSPRQRP
                     YVA"
     gene            15031..16086
                     /gene="glpX"
                     /locus_tag="PLU99_00705"
     CDS             15031..16086
                     /gene="glpX"
                     /locus_tag="PLU99_00705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOO15600.1"
                     /translation="MPVQQVSEAEYEPEKLLGMEFVRASEAAALNVFRWIGKGDKIAA
                     DRAATDALRGMLNLIDMRGTCTIGEGIKDEAPGIFVGERLGTWKDGSPCVSIAVDPID
                     GTKLTAKGLTGAIVVLAVARTDGPDSYALQAVPAVYMEKIAVGPKVKEGPGTVRLGAP
                     VDQNLELIALKLGKRVRDMVVCILDRPRHEQLIADVRRTGAAIRLIGDGDVAGAIAPS
                     MPDSAVDVYMGIGGSPEAVTAAAAIKCLGGEILARMWPRDEDERKKLEADGYGEQLMK
                     VYCTEELAKGDGIIFVATGITDNALLKGMQVKGHIARTHSLVMRAKSRTVRYVTAFHD
                     LTRKTIHLASEARESRL"
     gene            complement(16173..16883)
                     /gene="truB"
                     /locus_tag="PLU99_00710"
     CDS             complement(16173..16883)
                     /gene="truB"
                     /locus_tag="PLU99_00710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOO15601.1"
                     /translation="MDGLIILNKPAGITSAKALYRVRKLTGVRKSGHAGTLDPAAEGV
                     LILCLGAATKLVERLMDQPKLYRAVARLDVTSESFDSDRPLQPVQIERVPEAAEVASA
                     LGRFEGEILQVPPAVSALKIGGRPAYKLERAGQVVELAPRRVHVYSAQVLRYAWPELE
                     FEVACGRGTYIRALIRDLGAALHTGGCLAHLTRRAVGPFRIEDAWTLAALEEAGDPAR
                     YVIPLETARQLVRSRDTV"
     gene            17019..17546
                     /locus_tag="PLU99_00715"
     CDS             17019..17546
                     /locus_tag="PLU99_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOO15602.1"
                     /translation="MMLLTLTGCFGSGGSGGAGPAQDTDRATAGRRSDAARQYPLDSL
                     PTAVIQVKGQDVQVWLAQEFDAQRPHVVEEGLMHVPPEEIGDAQGMLFVFSEERLRGF
                     WMLNTITPLDIAFARWDGTVVKTWQMPPRTLRTFSSIEPAMFALEMKQGAFAHLGLQE
                     GDRLTIPDAVFKTQP"
     gene            17641..18828
                     /locus_tag="PLU99_00720"
     CDS             17641..18828
                     /locus_tag="PLU99_00720"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOO15603.1"
                     /translation="MCDVPPITVFETNGADCNSLHQRLAARGIATPRVPLDCAEGATL
                     DNVKIAVVALRNSAREAELQPAATLLERLVTDNIPTLVCGAADNLRWAGGPLVDWVEP
                     DATLDEIIGRLNTLTRYGPLIKRMERELDQLHRLGEQLNRYFSEIDQEMRLAGRLQRD
                     FLPATLPETAGAKLTALYRPASWVSGDLYDAFRIDEDHIGLFVADAMGHGVAAGLLTM
                     FMRQALVPKRVAGRFYEIISPPRTLATLHECLVRQKLPNCQFVTAVYAILNVRTRELR
                     LSRAGHPCPLHVQAGGEVAQINPAGGLLGLPDLDGEFEEARIILAPGEKVVMFTDGLE
                     PDFFAATEQRGAEAELSTAMRSWLSLPIEEFTEAVSAHLDLKEGSLYPPDDVTMLALQ
                     IDP"
     gene            complement(18962..19306)
                     /locus_tag="PLU99_00725"
     CDS             complement(18962..19306)
                     /locus_tag="PLU99_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOO15604.1"
                     /translation="MTANQPRKLYSQLLQDDPELRDVVADFVRRLPGRMEEIAEAFEQ
                     LDWGHLTMLAHRLKGATGSYGYPELSALAAAMERGFRAQQAEDFGQWLEQFAELVEAA
                     RAGLAEEGAEGS"
     gene            19535..21364
                     /gene="glmS"
                     /locus_tag="PLU99_00730"
     CDS             19535..21364
                     /gene="glmS"
                     /locus_tag="PLU99_00730"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOO15605.1"
                     /translation="MCGIVAYIGRREACPILLEGLKRLEYRGYDSAGMVVIRDNRLEM
                     RRAVGRISALEAKIHCELDGSTIGMAHTRWATHGAPTERNAHPHCDASGRLALIHNGI
                     IENYRALTAYLKTEGIELESETDTEVLAKLIGHLYQGDLADAVRKALNEVRGTFGICV
                     IHADHPDMIVGARRGSPLIVGIGNDEFIIASDASAIVQHTPQVIYLEDNEIAVVRRDG
                     TFRTMTLDAAPVTKEVSAIEWTLTQLELNGHEHFMAKEIFEQPESLRNCLRGRIDTAE
                     GRVVLGGLNGVARELVRAKRIILTACGTAWHAALVGEYLFEELARIPCEVEYGSEFRY
                     RNPILTDNTVVIAISQSGETADTLAAVREARERGALALGIVNAVGSTIARETDAGVYL
                     HVGPEIGVASTKAFTGQLAVLSMMAAYLARRRHMGHRECQDYLIALNEIPAAIERTLT
                     LSNQTREIAARYAKANNWLYLGRGLQYPVALEGALKLKEISYVHAEGLPAAEMKHGPI
                     ALITEDMPVVVIAPRDHLYEKVVSNIEEVRSRGGRVIAVATEGDDLMPRLADDVLYVP
                     KVPEPLQPFVTVIPLQLLAYHAAVRRGCDVDKPRNLAKSVTVE"
     gene            21507..21983
                     /gene="ribE"
                     /locus_tag="PLU99_00735"
     CDS             21507..21983
                     /gene="ribE"
                     /locus_tag="PLU99_00735"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOO15606.1"
                     /translation="MADTISARLEAAPHKFAIVVSRWNEFITSRLLDGAIDALVRHGA
                     EPGNLLRVWVPGAWEIPLAAQRLAGTGVYAAILCLGCVIRGQTPHFEYVAAEVAKGIA
                     QVSLSTGVPITFGIITADNLEQAIDRAGTKAGNKGADAALAAIEMANLLPQLSSLK"
     gene            22164..22640
                     /gene="nusB"
                     /locus_tag="PLU99_00740"
     CDS             22164..22640
                     /gene="nusB"
                     /locus_tag="PLU99_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOO15607.1"
                     /translation="MPRRPRPKHTQRSHARALALQALCLFDSLGEGFNEQLESFLHDS
                     QTYADLGIARSPGVSNLCFARDLARGAWQSRDRYDQLLRQAVADWSLSRMPPVDRNIL
                     RMGLHELLAQPDTPPEVVINEAVELAREFGSEDSPAFVNGVLDRIRKELGIGDSAG"
     gene            complement(22687..24708)
                     /locus_tag="PLU99_00745"
     CDS             complement(22687..24708)
                     /locus_tag="PLU99_00745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO15608.1"
                     /translation="MLRLGWPETVNRLAGVLASENKPARLAVALALADVPDPHDAYIE
                     PLISMLADPDADLRHAAAAALAGYRDSGVSARLRDMMLDVSQPAVLRLAAVEALGAIN
                     KRSAVAALVEAVALPDSSIAQPALEALERSTAMDFGGDAGRALAWWEQSQNEPLPDWQ
                     QGQIDRLVRRSRELEQDLRELELRLVESYRADYIRTPEAERGPLLQSYLKDPSALVRL
                     LGLELVQRQLGEGKPLPADAAAPVRELTRELLSDADPAVRVAAVQTVARFRDAADAER
                     FLSALAVERRANVCQALVNGLGYVGSGEACETLLNIVRSQDNPARAEAVAALGRQAER
                     GVLGAAERDAIADELLKLFAETPPAQAALRERVLWAMAQIADPAFATAFSQALCEREA
                     PLVRQAAARGIAAVKAQIEALIPVTRDADLTVRRTAVESLADCACTDEHLAALWERLA
                     PSSEPDATIRRISWQAAVKLLSGRSVAEVEGWLGRLPDTEERDAQVDELLAVLEASLA
                     KVPEGLRELGRVWTRVGAQRAAQNKIDAAIAAYLAAVRDLRMSQAPELTEAAQAVLRI
                     SLLNERYNESLASALAKGSPALDGGALWAAVRPELAARLNAGGAQQVIQMLSSLRQHP
                     PAAFSEEIANDMSALLAQAQAPPASAPASAPASAPATQAAQTQPENAGP"
     gene            25245..26150
                     /locus_tag="PLU99_00750"
     CDS             25245..26150
                     /locus_tag="PLU99_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO15609.1"
                     /translation="MKRAACCAAMIACVWAATPVWAGLYQDIYQGLYILSTPSGFPLL
                     TASDGTRINGQRSGRLQIQRNSPGRGYDVQFNRVFGLDSQGRPEVLDLGNFEIELQGA
                     TQATFGYTNRGPLIGTANFAANNLNYSLRAKSGIQNFTLAGTLNIAEAVEINQFGFYE
                     ASINISNTNSELFLDGLAQESVPENFDIGPINVKGNIFFDAFVALLSSLGVDTTELQA
                     LTPGSAIGQIVDEIALQVPQLVAGIQVSEDGQFAPPPLVLDAAADIATPGLAPAQTAP
                     GLTIPEPGTFLILGLGAVLWGWRRR"
     gene            26393..29620
                     /locus_tag="PLU99_00755"
     CDS             26393..29620
                     /locus_tag="PLU99_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15610.1"
                     /translation="MKYCWHLLFVGLLLACPGVARAYETGDLNCDGAVNVFDIDPFVL
                     ALTDPVEYAALFPSCAYLLADANHDGEVNVFDIDPFVQLLTSAPPTAACCNPAGGCAV
                     TTESGCDGVWHPEWADCTVADCPTPEPPIAMELAGNPLTAYPYFEYVRAFHVNAPIQM
                     AIDPTLRPEIVGRSADVYIVEKKSAGEWAVDPALVDETAGGPTTVTFGGTTIQENTFQ
                     ITGPNELSAAVFQAATGANTGLGRGYDMIVDMNQNGVLDGGDYIDGLGREAGLYVCHD
                     TTTAGPLAVTETLYNVGTIFGIPATVAGQDLYYPTNIASMGQLPLIVISHGNGHNYQW
                     YDHIGFHMASYGFIVMSHGNNTGPSSGLYASLTTCGHTDAFFHLLPSIASGALVGHVD
                     SHRIIWVGHSRGGEGVAFAYRRISAPGPDAYTPTYYTADDIILVDSMLPVDFFGPNRT
                     NPGAANYHLWTASGDSDVDGSAGCDLCQTFHIHDRATRFRGSTVVQGTGHGWFHDSEG
                     SGAWFSGPCPINESGTHLVQLGIMLPMYKYYAEGNVPGEDYLWRQYERFHPPSVPVGI
                     DPCYVVSQEYRNMGDEGYAFIDDYETQPGAGTSSSGGAVTYNVTNLTEGRLDDNNGSF
                     AWSSGDPFNGATQASSDYSDTSKGVVFDWNGANRYYEWAVVPTLRNFADWKYVSLRGA
                     QGTQHPYTQATNGILTFTITLRDSAGNTSSISTGAYGGGFGMPYARSGGWHNEMRRIR
                     IRTADFLANGSGIDLENIVAVRLDLGPDWGTPQGRIVIDELMLDNDLPPFFIPLTMYV
                     AEPPPEFIPPHAPYPVNVEILPGNDTLVPGSAMMYYRYDGGDWQSQPLVNVAGDLWRG
                     TLPAPQCGDRPEYYFEAEGAVTGLLSAPLGGAAAPYSSLVGTYISLLQEDFETAAGWT
                     VYNTPGMTSGMWQRAIPGGHADHSPPEDYDGSGQCYVTDNRSTFDVDGGPTQLISPLL
                     DLSGMEDPVVRFADWVYCDDATGMYPSQQDYLDVYVSSDDGATWVQAAHIANHDNWMV
                     HEFHVVDFIPLTATVRVSFWSQDNPNNSMTEMGVDAVSIFDVQCD"
     gene            29673..30275
                     /locus_tag="PLU99_00760"
     CDS             29673..30275
                     /locus_tag="PLU99_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15611.1"
                     /translation="MKAILASVCLLTMVSMVFAAPPVAKTPAAVDEVVYARPFTLSES
                     FRYDWCNAPFQVSEGTILVLKVQTNLVIPRQIEEPVLYVGNQAAMRLNQGDKSGHLIV
                     VVPGEVDLTKDLVWFGTPELPGRVTPGQIEAQRRLADQAGIKPLSAEKAKVATDNGGP
                     RVKAKDMTTLLRDVIAGLVDQYSPQEKVLSETWRMEPLSN"
     gene            complement(30386..31501)
                     /gene="aroB"
                     /locus_tag="PLU99_00765"
     CDS             complement(30386..31501)
                     /gene="aroB"
                     /locus_tag="PLU99_00765"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOO15612.1"
                     /translation="MKTLNVELAGGGYRVHIGAGARSALSELANAPCGRFPPAHKPRI
                     TIITDKTVAELHLEPVLEVLRHADRSTCPLVLTVAPGEASKTLDVAASLYQALARGGV
                     ERSDLIVTLGGGVVGDLGGFVAATWHRGIRFIQVPTTLEAAIDAAVGGKTALNLAAGK
                     NLVGVFHQPAAVFIDTDFLHTLPQRDFIAGLAESVKHAAIDGEAFLEWQERQAQSILA
                     RDPAVLEELIAWNCRIKAAIVAQDEREHGLRAVLNYGHTAGHAIEHLLGYELRHGECV
                     GLGMIVAGELSCRQAGLARARAERITSLIARFGLPTHLPRRVDGAEIAATCRLDKKAR
                     AGTVRFVLLEGIGRPRPGYSVCETELVDALKVIGPGG"
     gene            31926..33161
                     /locus_tag="PLU99_00770"
     CDS             31926..33161
                     /locus_tag="PLU99_00770"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOO15613.1"
                     /translation="MFRLLKELGNPQDKIKAIHVAGTKGKGSTCHMIAAMLQNAGYRV
                     GLYTSPHFTDLRERICVDGERISERDFTRIIAKIAPVVRRLERDEPTFFEVVTAAAFL
                     HYVNKKVDLAVIETGLGGRLDSTNVVKPLVCAITSISHDHVAQLGNTLEKIAEEKAGI
                     FKPGVPVITTRQPAGVKKVLRAAAEDKKCDLRIVGEDLEFSYRFECSRATGPHIRVCM
                     STPTSRFDHLQVPLLGEHQAHNCGIALGVIDALRQRGYVIPEQAAIDGLAKVHIEGRL
                     EIIRDNPRTVVDVAHNASSIAALMRGIGQNITYDSMVVIFGCASDKDIDGMLGQVQLG
                     ADKVIFTNIDSPRSADARELHARFTEKCFKMAQVADNLEEAYRIARSCVTREDLICIT
                     GSFLLVGEAKRLLAQGKLD"
     gene            complement(33199..34302)
                     /gene="prfA"
                     /locus_tag="PLU99_00775"
     CDS             complement(33199..34302)
                     /gene="prfA"
                     /locus_tag="PLU99_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOO15614.1"
                     /translation="MPVTKIADPPWMARVEEMTRRADELAAEMNKPEVASNGAMMVRL
                     AREHGELEKIARLYRDYRGLMREIEESQQIVDEPGSDDDLRQLAEEQMPGLLARRERL
                     LTQMQERLVTGNEASIRSIILEVRAGTGGEEAALFARELLDMYLAYAARKGFKTEVMS
                     LSDSDLGGVKEAIVNITGEEVYMHYRFEAGGHRVQRVPKTEAQGRIHTSAATVAVLPE
                     PEEVEVDIDWEKDVIEYVSRAGGPGGQNVNKVSSAIRLEHKPTGITVSMRDEKSQHKN
                     RAKARRVLMSRVYEHYASQQRAQIVQDRRSMVGSGDRSDRIRTYNFPQDRCTDHRINK
                     DLFGLQKILVNGEFDEFRAELHDRDLKLRLEQL"
     gene            complement(34460..34699)
                     /gene="rpmE"
                     /locus_tag="PLU99_00780"
     CDS             complement(34460..34699)
                     /gene="rpmE"
                     /locus_tag="PLU99_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOO15615.1"
                     /translation="MKKDIHPNYVETQVSCACGASFKTRSTVPVLKVDICSECHPFYT
                     GKQKFVDTAGRVERFQKKFGGNYFKKDTEQKPGVK"
     gene            complement(34891..35268)
                     /locus_tag="PLU99_00785"
     CDS             complement(34891..35268)
                     /locus_tag="PLU99_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO15616.1"
                     /translation="MAKILVIDDEPIPCRMLHHFLTGEGHEVRTALDADEAVQVAHDF
                     RPDLLISDWLLKDKTGLDVALAVRDELPEARVIFTTGLPATVVAEKARAASNVSVLEK
                     PFDLESLVAAIRRSMQAANVGEC"
     gene            complement(35391..35927)
                     /gene="def"
                     /locus_tag="PLU99_00790"
     CDS             complement(35391..35927)
                     /gene="def"
                     /locus_tag="PLU99_00790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOO15617.1"
                     /translation="MPQTTKLDPGKLAVVHYPDPRLRQPCEPVAVFDESLAALADRMI
                     ELMVQNKGVGLAAPQVGVLKRLIVVSPTGQAADAMALVNCVIRDREGNVEAEEGCLSL
                     PGVNVRVRRAKRCRLEALDLAGNPLELEGEDLLARIWQHETDHLDGVLIIDRMGPADR
                     MAVRKTLRALEEGYNGAT"
BASE COUNT         6683 a        11906 c        10873 g         6666 t
ORIGIN      
        1 ctacctcggg ttcactacct ggctcggggc gaagatggcc ggccgccagg ctaccatccg
       61 ggatttcttc ctcggcggac gcaagctgcc ttggccggcc gtcagcggct caatcatcgc
      121 taccgaaatc agcgccctca cctttgtcag cgtgccttgg gtagtcttca aacccggcgg
      181 caatttgacg tatctgcaac tgggcgtctt cggttcactc ctggcgcgga ttctggtcgg
      241 ctatatcctc gtgccggcat actataagcg cgaaatctac agcccttatg actacatgca
      301 caaccaactc ggtgggcacg tgcgctgtct caccactggt ctgttcatgc tcggcggcct
      361 gctggggcag tccgcacgca tttacctgac ggcggaagtg atcaatgtcg tgctgcacga
      421 ccaactggtg gcgctctcgc aagggttagg cctgaacgag ttggcttggg ccatcatcct
      481 cattaccggc gtgtccgtgg cctggacttt gatcggcggc atgagcaccg tcatctggac
      541 cgatgtagtg ctcttcctgg ctttcctact gggtgcgatc accgcgctcg ccgtcgtcgt
      601 cgcctccctg gacggcggtc tgctcgaggt gatccgaacc ggctgggcgg ccaaggagtc
      661 cggcccctgg ggcaagttca ctttcttcga tttcagcccc agcccggtgc gcagctacac
      721 gatctggacc gccgtcattg ccagcacgtg gggcggcctc ggctcttacg gcacggatca
      781 actgatggcc caacgcatgt tctgctgccg cgattatcgc gcggcgcgcc gggccctcat
      841 cgccagctcc accagccaga ttgtcacgtt cattgtggcc ttcgtgggca tcggtctata
      901 tgcctactac cagagccatc cgctgcaggg cgaggcgctg gacctgtttc gcgccaaagg
      961 cgaccgcatt ttcccaatct tcactgtcga ggtggtcccg cgcggcctga agggcctcat
     1021 tatcgcagcc atcttcgcgg cggccatctc gagcgtcatg ggcatcttga ccgccctcag
     1081 ccagacggcg cagaccgcct tctacgagcc gttgatgcgc acattgcggc gacgcaagca
     1141 gcgctcggcg ggcgcccctc tgtattcccg acagccattg gccggctctc gaatcggcgg
     1201 cgcgactacc gtggccgtcg ctgcggaatc gtccacccgg cacggcgacg acacgaccga
     1261 ggagcccggc cacaaccagg ggcgagcttc ggtcatggca agtcgcggcc tggttgtgtt
     1321 ctggggcata ctgctcggat tactcgcata ctggtcgcag caagtcgcaa gcagataccc
     1381 gtccattctc gatatgggat tggccatggc gggttatgtc tcgggagcgc tcctggccgg
     1441 gttcctgttg tcattcctgc cgctgcgcgt cgacagccgg ggcttcattc acgcggcgcc
     1501 gctctcctgc atgtgcgtct tcgcgttgat ctggcacgcc gaatggtcca atgtagtctg
     1561 ctgggtcgca gccggggtca ttttcgccgg ctggctgctg caactttacc tggatttcct
     1621 ctcgaaacgc ggcgagcgcg cccgcactgt gcttccagca ccactgcaga cgctggtcct
     1681 cctggccgga ctggcattta tgctatggtt gaactatcgg ggatactggc cagacccgac
     1741 cggcggcact aaacatcaaa ccgtcgcctg gccctggatg ataccgctgg gcagtactgt
     1801 agcatttgtg tggggctatc tcctggcgcg cttccgtcct tgtccagagt agctgttggc
     1861 ctaaagcagc aagtccctaa gaccttgctc aatcctagct agactatccg aaatattctg
     1921 ttacatcaaa ttcacccaat atttagagcg ctgtcagacc gccatgcgaa actgtaatag
     1981 aacgcgcctg ttaaggcctg gctcgaatcc aaaagtgtgc aaatgcttta ctgggcaata
     2041 gtctttttcg tcatcgctat tatcgccgcc atctttggct ttaccggcat cgcgtccggg
     2101 gcggcgacta tcgccaaaat actgttcttc ctgttcgtgg tcctctttgt cctcgcgctg
     2161 cttgccggcc tcttgcgagg ccgaacgtaa gtggccgacc ggcctacgca cccggcggcc
     2221 aacaacaaaa taaaacgaag ttcgccacgg cggcgaactc aaccggtgca aacaggggag
     2281 ccgtgaatag gaacgcccgc cccgtggcgg gcgctcctta ttccccgctc gcgccagcgt
     2341 ggatggatcg cgggctggga ccctccaagg ctcactgcgg ccccacgggc gcgcgaaaac
     2401 gctcgggaga gaagaaatga agaagttctg gcagccactt gtgacaatca cgatcggcgc
     2461 ttcctgcctc ttggcacttg gcctggcggg ttgcaacagc gccaacaaat gtaacagtga
     2521 aaccacctgt gcgccgcggg ccgcctacct ggccgagtgc ccacgctgcg cggtgtgcga
     2581 gcaccgaagc agtccctgta gtgcctgtac ggccagttgg cgctcacgct gcgaacgtga
     2641 aacgtgcagc agcatatacc tgcccaccgg cgaccgcagc gatgccgtgc tgaaactgga
     2701 aaagtgcggc ccccgcgaag tccgcgccgg agagccgttt aagtacacga tcaaagtcac
     2761 taaccgcacc aaacacgcac tgcgaaacgt cactgtaatt gacgaactgc ctcaggatgt
     2821 cagtgtccag gggccgatcg gcggtggcgc aggctggctc ccgcatgacc gcccggccgg
     2881 cgcccacgcc gccaacgtcg tccgctatcc ggtcggcgat ctcggcccgc gcgagtcgcg
     2941 ctgcatcgag caatgcggtg ttgccggcgg caccggttgt ctctcgagct gcctcaccgc
     3001 ccgctacgac ctcttcgcct gcctccagac cgccgtaacg cagccgcagc ttgcgctctg
     3061 catgaccggc ccgcaggatg tgttcatctg ccgctccgac caggacgcca acctgcacgt
     3121 caccgtgcgc aacaccggca gcgcaacggc cgacaacgtg cgcgtaaccg tctcgatcat
     3181 gccgcccgcg ggcgcccctc agttggccgc cgccgccgac tcggagcgca tggtcggcgc
     3241 gctcggctgc aacgaggaac gaagtttcga catctgtctg cccgtgctcg cccccggcga
     3301 ataccgcgtt gcagcccagg ccgtcggcga cgccggcctg cgcaccgagc gcagcgaact
     3361 gttgttcttc gcacgctttg cggccttgaa catccgcgcc gccggcccgg atcaagagct
     3421 catcggcctg cccatcgaat accgcattct gatcgagaac accggcaacg cgcccgacac
     3481 caatgtgacg gtcaccgccg aactgccgcc tggcgtccta ttcctcgaag ccaccgacgg
     3541 cggtcagcct gccggcgagc gcgtcgtctg gaacatcggc aatttcccgc ccggccagcc
     3601 ccgcgaattg ggcctgcgcg tgcgcgcgac tgacgtggcc cgcaccatca acccggtctt
     3661 tgtcgtgtgc ggcgattgcg gcgggcagca gagcgtcatg ctggccacag cgctggaagg
     3721 tgtgccggct ctgctgttgg agatgatcga catccaggat ccgctgcgca tcggcgaatt
     3781 ggtcgagtat cgggtcacgg tcatcaacca aggctcggcc cccgaaacaa acatccgcat
     3841 ggccgcccgc ctgccggacg agctgacctt cgttcgcagc actggcccga cggagccgcg
     3901 cgtcgagggc actgaactca cgttcgccac ggtgccgctg ctgcctccga aggaaaagat
     3961 cacttggacg atcttcgccc actgcgaccg gcccggcgat atgcgtatgg ccgtgcaggt
     4021 tcaaagcgac caggcccagc gcccgatccg cgaaactgag gcgacgcggg tttactagag
     4081 caggtaacaa agcgtcggcg cacgattcgg cagcgcgttc cggcggggtc tgccttcccc
     4141 cccgccgttg ctgccgaatc gtgctcgacg actaatagtt ccagcggggg ccgggggtga
     4201 aatcttcaaa cacgtccgcg ccagtgccct gaagccggct atcccccgcg ttgcgccctg
     4261 ctgttgagga tgcgttatca tgctgccgtt tgccacagca caggagatcg catgctggga
     4321 atcatcggcg gcacgggact ggcggatgca ctgttcgggc aagtcgacgg cgcggatcac
     4381 gtaatcgaca cacctttcgg cgcgccgagc gggccggtac gcgtggtaca ctggcaaggt
     4441 ctggaagtgg ccctgctggc gcggcacggc gcggggcacc taatcaatcc ctcgcaggtt
     4501 ccgtatcggg ccaacatcta cgcgatgaag tcgttggggg tcacgcacct gatcgccagc
     4561 ggcgccgtcg gatcgctgcg cgaggagttc cgcccgcgcg aactcgtcat tgtcgaccag
     4621 gtcatagacc gcacgtatcg ccgtgtgcct acgttcttcg acgcgggact ggcggcacac
     4681 gtggaattcg ctgaaccgtt ttgccccgtg ctgcggcagc ggcttctggc tgcggccacg
     4741 cagatcgaaa ccaaggtaca cccgcacggc acctacatct gcatggaagg cccggccttc
     4801 agcagccagg ccgagtcgcg aatgcaccgg gcgtggggcg gcgacctgat cgggatgacg
     4861 ctgatgcccg aggccaagct ggcgcgcgaa gccgagatcg cttatgccgc gatcgcgctg
     4921 atcaccgatt acgactgctg gcgcccgcac gcacccggcg cgaccaagca gaagctgctc
     4981 gaggaaatcc tggacaatct ccacgctgcc acgcacaacg caatcgccct gctgcgcgcc
     5041 gcgctggccg acttcgcgcg gcaaccaccc ggagcgtcgc ccgcccatag cgcgctggag
     5101 cttgcgatct ggtcggatcg tgcgaaaatt gcgcccgaaa ccgtgcggca gtacggagta
     5161 ctgctcaagc ggcagcttgg cgagcaaaag cagaatggct gatacgaagg cgcgggaaag
     5221 tcgcaaaaag gaaaagttgg aaggtgcgcg cacactttcc ggctttcgcc ctttcgcacg
     5281 ttcccgatga tccccgaggg cgagcatgga ggcgtttcgc aggccgcggt tccggggcga
     5341 gtgcgagtgg atcatcgctc agccacaccc tgaccgcgac cgcctcgcaa gccaggccgg
     5401 cattgcccct ctactcgccc aagtcctgct caatcgcgat atctcgacag ccgcgcaggt
     5461 cggccggttt ctctccccgg atttgggcct cctgctgcca ccggaagctt tgccaaacgc
     5521 cgtcagcgcc ggccgccgcc tggccgaagc cgtgcgcgcc gggcggcgca ttgtcatcta
     5581 cggcgactac gacgtggacg gcatcactgc cacctcaatt ctctggctcg cgcttcgcct
     5641 ggcgaacgcc aatgtgagtt tttatatccc caaccgcctc gaggaaggtt acggcgtcaa
     5701 cggcgaagcc ctccgctcca tcgcggccga aggtgacgcg ctggtcatca cggtcgacag
     5761 cggaattacg gccgttgccg aagcgcgtct ggctcggcaa ctcggcctcg ggctgatcat
     5821 aacggatcat cacgaaccgg gtcccacgct gccggaatgc gactgcgtgg tgcatcccac
     5881 cgcttgcgga gccagcgaga atccagacct gtccggggcg ggcgtagcat tgaagatcgc
     5941 ctgggctttc gcacaggagt tttgcggcac gcagcgcgtg tccgaacagt tccgcaactt
     6001 cctgctggat gccacgactt ttgccgcgct gggcctggtc gcggacgtcg tgcccctgac
     6061 cggcgaaaat cgcgtgatcg ccgcgttcgg cctcaagtat ctgcgccgta cgcaaaaccc
     6121 cggcctgaag gcgctgcttc aagtatccgg cttggagggc aagtcgcgtt atgacgatta
     6181 cgacgtcggc ttcatgctgg cgccgcgcct caatgcggtc gggcgactcg ggcacgctcg
     6241 cacggccgtc gagttgttca cgcaggcaga cagcgcccgg gccgcggaga tcgcagccgc
     6301 cctcgacgcc cagaaccgca aacgccagga actcgaacgc acaatagcaa agcaggccga
     6361 ggcgctcgtg atcgagcgtg gcttcgaccg cgacggctgc cacggcatcg tgctggcttg
     6421 cgagcagtgg catgccggcg tcatcggcat cgtcgcttca cgcctggtaa accggttccg
     6481 ccgcccggcg gtgctcatcg ccttggagaa tggctgcggc caagggtcgg cccgaagcgt
     6541 gccacatttc ccgctgcacg aggtgctggc ctgctgcggt gaacacctgc tcagtcacgg
     6601 tggccacgcc atggcggccg gcgtgcgggt ggcagccgac cgcgtcgacg cgttcacaga
     6661 ggctttcctg gctgaggcgg cccggcggct gacgcccaaa gacctctgcc cgcgcctgta
     6721 cttggatgac gaagtcgagc tgaccgatct gacgatcgat gtcgtcgacg cgctgcaacg
     6781 gatggcgccg cacggaacgg gtaatcctgc cccgcggctg gcaacggggg ttgttgagct
     6841 tgccgagccg ccacgggtgg tcggaaaggc cggcaaccac ctgcaaatga tggtccggca
     6901 aggtcgcgaa caccgcaaag ccattgcgtt caaccgcggc gaccaggcgg agcaggtaaa
     6961 cgaggtgcgt cgtctgcggc tggccttcga gccgattatt aacgaatgga atgggcgccg
     7021 cagcgtcgaa ctgaaagtag tcgattggaa gttcggggac gtcagatgac gtcgtttgcc
     7081 acagcggctt acgattttgt aagcccgggc agggctcaaa ttgcgactcc ggcagtggtc
     7141 cggcggactt gcaaaccgcc tttaagggtg ctagaacccc atcagaaggg ccccagcggg
     7201 tatcctgccg gagccggtcg gcgatagtct ttaactgttg atgcgggcgc gaggtgggtc
     7261 gtgcggcgcg gcagagtggc cctacggaga acaaagtgga cgaatacctg aaactaaaga
     7321 cgctagtgga agagataggg gacgacgttt acaaggcgat gggcggcaac aaggcggctg
     7381 gcacccgcgt gcggaaagcc atgcaggaca tcaagaatac ggcacaagcc gtccgcaaac
     7441 gcgttctcga catccgtagt ccgggaggcg gggaccgcac cgatgccgaa acgccgagtg
     7501 gtcccgctcc cgatcagggt ggcggtgatc aacagtaagt aaaccaataa ttaatcagta
     7561 ataaggttac ctcatagtcg ggggatgaat gaggtcttaa tgccgccgcc cgccattctc
     7621 gatcctttgt cgttggattt ctcccgccct tttgccacgc gcgagcagat cgcggaagtc
     7681 aatccgcacc gccatgagtt cgcgctgctg gatgcggtcg tcacgttcga tcgcgaggcg
     7741 ggcactttct cgggttacca tgacgtgcgc ccggacgaat ggtgggcgcg cgggcacata
     7801 ccggggcgcc cgctctttcc cggcgtgctg atgatcgagg tcgccgccca gcttgccagc
     7861 ttcctgggcc acctcgtgaa cgggcgggat ttcttcatgg ggctcactgg cgtcgatgat
     7921 gtaaaatacc gtggtacggt cgaaccgccg tgccggttcg tcgttgtcgg ccgagcgtta
     7981 gatgctcgta agcgacgtac caagtgcgcg acgcagggct tcgtcgacgg caccatggtt
     8041 ttcgaagccg tgataaccgg aatggccttg tagcaaagcc ggggcggcgc cgtggtgcag
     8101 gccggggacg cacgcaagcg ctgagcacgg gcttgccgtc atcgctgggt tcgtgctcgc
     8161 ccgggtgcga ggcgcatggg aacacagcgc tcgccgcacc cgagctttag agacaagaat
     8221 tcgcctttcg ccatgtccga caacaggagc gttagctcgg aagtcctcgc ggctctgccg
     8281 gaagcggtca cttggtcgca tcgcccccag gagagcgatt tagaggacgt ccctggatgc
     8341 ccggccgtct acctgttgct cgcgacctcg gacctgccgg tccaactggc cacgacccaa
     8401 catctccgcc gaatactgca ctcgcgcctc accgatgccg ctcgcgtcca gcagggtaag
     8461 gcggacctgg ccgaagtcgt ccgcggcgtt cgttggcggc ggatgcattc ccctttcgag
     8521 gcccgctggt ggtactaccg gctggcccgc gaaatgtatc cgaagcgcta ccggcggatg
     8581 atttctttcg gaccggcctt ctttctccga gtcgactggt ccgaaccgat tcctgacatc
     8641 aacgttacca agcaggtctg gcgtatttcg ggggaaatgg tcggtccctg gctgactcac
     8701 gacacctgtc aaaaggcgct ggagggtttg cgtgacctgt tcgacctgtg ccgctacccg
     8761 gaacaggtgc aacgcgctcc gcgcggccag cgctgcctct atgccgaaat gggacgttgc
     8821 gacgccccgt gcgacggatc agtgccgctg gagcgctaca ttgagcgcct ccgggcggct
     8881 tgggcgtttg cgcgaggcga agtgcgcccg tggctggccg aggcaaagga gcgaatgacg
     8941 agcgccgccc gcgcacagga gtatgagcgc gcggcattgc tcaaacggca gatcgcctgg
     9001 ggctggaaat ggcatgacca aattctgccg cgggtccatc cactggagca gttgaactac
     9061 tttctggcga ttccagccca ccgccgccgt gcgtgccgcc tgttcctatt tcgaagcggc
     9121 aacctggtgg aaggccctgt cctatcggac cgtaatgcac cttcggaggc gttgccttgg
     9181 cttgcgaacg tgcttgcgaa tcctcccgcc ccggcggacc cgaacgtgcg catggagcag
     9241 acctggctgg tggcacattt tctaagcagc agggccggtg aggcttcgtt ccgcagcttg
     9301 ttgcccaaag gtgagcctac tggcgatctc gaagagcagc ttcgccaata cctcgtacag
     9361 ttacggacca agcccgggga cgagggcgag cacgcggccg tgcgtccccc aagcagcgat
     9421 aacgaaaacc agaccaagta atcaacctat tcggaaaact cgcgtctaaa cgcgaattga
     9481 cttgactcat ttcaaatcca gattcatact ctagtgtaca gatcgcgcga ggcttaagtg
     9541 agccggctag tggcggccac ccaacggagc ccggcgtaat cggaattgga gagttattgg
     9601 cgcagatagt gcgcctgtgc gatacgtgcg gctaagtcag atccccgagc cgccggtttt
     9661 gtggtgcatc gttgcggggc gaagctgccc gcagaaaaaa gggtcgtcac cactccggca
     9721 aggttctcgg aggttgactt ggccgctttt tttgtgcaac caccgcggcc cgccaatcgc
     9781 cgcttacgct ttcactacgc gcccggccgg ccgcttcttg ttgcccgtgg cattgcgcgt
     9841 gtcgaccacc aggcgcgagt gctcgaccac catgtcgtag tcgactgagc tatggtcagt
     9901 cgaaatcaat acgcagtcat atttcttcaa attggcgcgc gtcaacggca cactgcgcat
     9961 atcggtgatg ttgtgttcac gcatcggttt ggcgctcgga atgtgcgggt cgtggtaatc
    10021 gactatcgcg ccgcgcgccc gcagtagctc gatcagcgtg acggacggcg attcgcggac
    10081 gtcgtccacg tccttcttat acgccagacc cagcaccaga atgcgcgccc cctttacggc
    10141 ccgcccacgc tcattcaagg cttccgacac gcgcataacc acgtattcgg gcatgaacgt
    10201 gttgatctcg gccgcaagtt cgataaaacg cgtggggcgg ccgtattgcc gcgctttcca
    10261 cgacagatac agcgggtcga tcggaatgca gtgcccgccc agtcccggcc cggggtagaa
    10321 agcctgatag ccgaaaggct tggtccgcgc cgcctcgatc acttcccaga cgtcgatgcc
    10381 catgcgctca aaaatcagct tcaactcgtt aaccagcgct atgtttacgg agcgataaac
    10441 gttctcgagg atcttgcacg cttcggccac ctcgcagctc gacaccggga cgacggttgt
    10501 cagcgcctgc ccgtagagcg tcgcggccag cttgccgctg gccggagtgg ccccgcctac
    10561 caccttgggt atgttgttcg tcgtgtagtc cttgcgcccg gggtcctccc gctcgggcga
    10621 gaacgccagg aagtaatcgc ggtccagccg cagcccggtc gcatccagca ggggttgaac
    10681 aacctcgcgc gtcgtgccgg gatacgtggt cgattcgagt atcacgagct gccccggacg
    10741 caggcgccgc gcgatatcct cgctggtcga gcgaatgaac gtcatgtccg gctcgcgcat
    10801 tttgtcgagc ggcgtgggaa cgcagataag gatcgcgtcg ggcttcgaaa gcagcttcat
    10861 gtccgccgta ggactaaagc gcttgtcctt gatgaacgga ttgaagattt ccgccggcag
    10921 atgttcgatg tagctcttac ccgccttcag catcgacacc ttcttcgggt cgatgtcgaa
    10981 gccgatgacc ttgaagccgg cgttgcagaa ttcccgcacc agcggcaggc cgacgtaccc
    11041 cagcccgatc acccccacga tcgcctcgtg ggcttctatc ttccgcgcta attcctcgga
    11101 cagtttcatc gcaagctcct gagattggtt cggctttgta ctcgcagcgc gaacgtggat
    11161 caaggcatcc agggttaagg aatcaaggca gataccgcgc gcctggttct aagtctcggc
    11221 gccggccccc ccttcgctct ccgctttgaa acaagcctgt gcgtctgctg cacccccgaa
    11281 atcggccgtc gccttgtcct cgtcatcctc cccctgctgc gcctcaaaat atgccttgat
    11341 cggattcgga aacttctttt gctggcacag cagctcgacg atctcgtcga atttcgcgat
    11401 tttctgattg tcgatccgca gccgcttctc ctgccctccg cggcggtagt acagatcgac
    11461 ccagcctttc ttgttgtaat cccgaatcga agcaatatcg gccagcgcaa tgcgcacgcc
    11521 gccgtaggtc atgccctgct cgtcaatttc ggcccgcagc cgcccggccc ggaacgcccg
    11581 gatcagaaaa taaaccgcca gaacgcacgg gatcaccccc cagtaatact ggttcctgat
    11641 ctcctctttc gaccacttcc acgcttgcca ctcggccagc tccgaccgca cgcggccgct
    11701 cggatcaatg gggacggtaa ttacgccgta aacactcgca aatcgctcca ccccggcggt
    11761 cgcggcgccc tcgcgcggag caagcggctg ccccaatagc tcgtgcagct cttgccggct
    11821 ggcgaccttc cgcgttcgca gcagatggta ctcctccggc cccggcgtct cgcccaactc
    11881 gggctgtccg gcagtccata acgaaagccg ctcacgggct ttggcgatgt tctgcttgcg
    11941 gtatcccaca gcgccgtcgt atatgaaata cccccccatc accaggaata cgaggccgac
    12001 acccagccac cagttccgat ctgtacgggc cggtccactt tcgagcagaa tcatagggca
    12061 ctcccccgcg ccgccggaac gcctttcgat cggttagcgg cctggcaggc acgggttttt
    12121 ctgcaagctt cgatcctcgg catcctaata tcaactccag ggacagtcga gagccccctc
    12181 gcgccggcgg tttgaatttg ccttgaccgc cgaataggct taacggtttc aatggaggtt
    12241 tgccacgtgg gcgcgaccgt caaacaccga ccgtgcgttg tgataattcg cgatggctgg
    12301 ggccgcaatc ccgatcccaa cttggattat gcaaacgccg tcaggctggc cgacccgccc
    12361 gtcgatcgca tgctgaacga gcaatggccc acgaccttga tcgcgaccag cggttcggac
    12421 gtcggcctgc cgcccaacac catgggcaac agcgaggtcg gacaccagaa catcggcgcc
    12481 ggccgcgtcg tcgaccagga atccgtgcgt atctcaaaag ccattcagga cggctccttc
    12541 ttcggtaacc cagaactggt cgcggcggtc gagcgctgcc tggcacgccg ctccaagctc
    12601 cacatcatgg gtctggtgag cgacgaaggc gtccattccc ggctcgagca cctgttcgcc
    12661 tgtgttgagc ttgccgcacg ccgcggcctt aaagaggcct acatccactg tttcaccgat
    12721 gggcgcgact cgccgcccac cagcggcatc ggctacatcg agcgcgtcga gcaggagctg
    12781 aaccgcatcg gcgtggggca gatcgcctct gtttgcggcc gctattgggc catggatcgc
    12841 gacaaccgtt gggaccgcgt gcgcaaagcc tatcgcatgc tgacccaggg cgaaggccgg
    12901 gccgcgccca gcgcccgcgt ggcgctgcaa cgctattacg acgacccgct cgaaccaaac
    12961 atgcagggcg acgaattcgt agtccccacg gtgattagcg aagacggccg cctgccgctg
    13021 gcgacggtgg gcagcggcga cgccgtgatc ttcttcaact ttcgcggtga ccgcccgcgc
    13081 gaactgacca aggctttcac gctcgacaaa ttcccttatc gcgccaaaga caaggccggc
    13141 gtcgaacgcg aaatgggctt cccacgcccc cagaagctgg acctgttttt cgtcaccatg
    13201 acttcttatg aggccgacct gccggtcaag gtggccttcc ccaagctgcc cagaatggag
    13261 aacattgccg gcgagtacct cagtcgcctc ggcctgcggc agttccgctg cgcggaaact
    13321 gaaaagtacg cccacgtcac tttctttttc aacgactacc gcgaagagcc cttcgctggg
    13381 gaagaacgac agctcgttcc ttctcccaca gtcgcaacat atgaccagca gcccgaaatg
    13441 tcggccccgc aagttgcgga tgttgtcgtg cgccgcatcg aaagccgcct ggacgatttc
    13501 gttctggtca actttgccaa tcctgacatg gttggccaca ccggctcgct accggcggcg
    13561 gtgaaggctt gcaaagtcgt tgatgagtgc gtgggccgag tattggaaac cgtcaagcgc
    13621 ctgggcgggt gcgccatcat cactagcgac catggcaatt tcgagcagat ggtcgatccc
    13681 cagactggcg gaccgcatac ggcgcatacg ctgtatccag tgccgctgca cgtctatggc
    13741 gctggctttg agggcgccga actgcgcgat gacggccgtc tggccgacgt catgccgacc
    13801 gccctaagca tgatggggct cgacattccg cctgaaatga cggggcgttc gctgattctg
    13861 taggcggggc gtgcaaagct ccaactctgc ggcgcgctaa acaaaagagt ccgaggctct
    13921 ggttttgttg aacgccccat aaccacattt caaacgatcc aaatgctcat tttttgcttg
    13981 cctttttcgg gccggctgtt ctagaatttt gccagaggag atggaaggag caactgacgg
    14041 cccgcgctgg ccctcctccc agagtgcaac taaggaacta gcgagccaaa cgcagggatg
    14101 cgaggtgtgc ttctgcgcac ctgcctcgcg attttcttgg agggatgagg acgtgccaca
    14161 tcgtcgcgtc gcagacgtct tcgtcgacgt ttgcactcag agagactatt tgagttcgga
    14221 cggcgcccgt cccacggcca acgcccgcac cgtccagcaa aacgttaaac atctgatggc
    14281 cttcgcgcgc tgggcaaaaa tacccactat ttcttgtgtc gatgcgcgcc gtccggatac
    14341 ggtgcgcggc gtgccgcacc cgcattgcgt tctgggcacg ccgggacagc gcaaaataac
    14401 tttcagcgtg ttgccggatc atgtcactat cgagtcagat aacctcctgt gtatctcgct
    14461 cgaaatcctg cagcagcacc agcaagccat cctgatcaaa cagcatcgcg accccttcac
    14521 caatcccaag ctcgaccgtt tgctgaccga agtgccgacc cagcatttca cggtcttcgg
    14581 cgtcgccctg gaggcctcta tccgcctcct cgtgctcggc ctattgctgc gccaccgcca
    14641 cgccaccgtg atccatgacg cctgcggctt ctggaacccc agcgaggccg acatggccct
    14701 gcggcaattg gccgcaaaag gctgtgaaat ccgcagcacg cacgaggtca tccgcgagcg
    14761 cctaacccaa ctccgtaccg gagctcgcag cccgcgccag cgcccctatg tagcgtaact
    14821 gagccgcgtc gcgcggccac tctgcgacgt gtcgctggaa ctgacgatta accgccgcaa
    14881 cgcatccttc ggggccgttc tcttccggaa ttaatggacc gtattcgcag ctctggatgg
    14941 aacgccgcgc agtggacgcg cctacacttg cgcattaaag ttactgcggt ggctgcccac
    15001 gtccacgcgg ccgccaaagg agatgtgcac atgccagtac aacaggtttc tgaggcggag
    15061 tacgagccgg aaaaacttct aggaatggag ttcgtccgcg ccagtgaagc cgccgcgctc
    15121 aatgtcttcc gctggatcgg caaaggtgac aagatcgccg cggaccgcgc agccacggat
    15181 gccctgcgtg gcatgctcaa cctgatcgac atgcgcggaa cgtgtacgat cggcgagggc
    15241 atcaaagatg aagcgccggg catcttcgtc ggcgagcgcc tgggcacgtg gaaggatggc
    15301 tctccttgcg tcagcatcgc cgttgacccg atcgacggca ccaagctcac cgcgaagggc
    15361 ctcaccggcg ctatcgtcgt tctcgccgta gcgcgcaccg acggccccga ttcatacgcg
    15421 ctgcaagccg tccccgccgt ttacatggaa aaaatcgccg tcggtccgaa ggtcaaggag
    15481 ggccccggca cggtgcgcct cggcgcgccc gtcgatcaga atctggagct gatcgccctt
    15541 aaactcggta aacgcgtgcg tgacatggtg gtctgcatcc tcgaccgccc gcgccacgaa
    15601 cagctcatcg ccgacgtccg ccgcaccggc gccgccattc gcctgatcgg cgacggcgac
    15661 gtggccggcg ccatcgcacc ctccatgccc gacagcgccg tcgacgtcta catgggcatc
    15721 ggcggctcgc cggaggccgt taccgccgcc gccgccatca agtgcctggg cggcgaaatc
    15781 ctggcccgca tgtggccgcg cgacgaagac gaacgcaaaa agctcgaagc cgacggctat
    15841 ggcgagcaac tcatgaaagt ctattgcacg gaggagctgg ccaaaggcga cggcatcatt
    15901 ttcgtcgcca ccggcatcac cgataacgcg cttctgaagg gcatgcaggt gaaaggtcac
    15961 attgcgcgga cgcattcgct ggtcatgcgc gccaagtcgc gcaccgtgcg atacgtcacc
    16021 gcgtttcacg acctgacgcg caagacgatt cacctggcca gcgaggcccg cgaatcgcgc
    16081 ctctagcaga gccggggtgt gtcgccgcga actgtcggcg acgttcatcg acttcggagc
    16141 aaagggagac cggccaaacc ggtctcccag cgctacacag tatcgcgact ccgcaccagt
    16201 tggcgcgcag tttccagcgg aatgacgtat cgagccggat ctcccgcctc ttcaagcgcc
    16261 gccaacgtcc acgcgtcttc aattcgaaac ggaccaaccg cccgccgcgt cagatgcgca
    16321 aggcagccac ccgtgtggag cgctgcaccc aggtcgcgga tcagcgcccg aatgtatgtc
    16381 ccccgcccgc aggcgacctc gaactccaat tcgggccagg cgtaacgcag gacctgcgcc
    16441 gaataaacgt gcacccgccg tggcgccaac tccaccacct gccccgcacg ttccagcttg
    16501 tacgccggcc gcccgccaat cttcaacgcg cttaccgccg gcggaacctg caaaatctca
    16561 ccctcaaaac ggcccagcgc cgacgctact tccgccgctt ccggcacacg ctcgatctgc
    16621 accggctgaa gcggccggtc gctgtcgaaa ctctcgctgg tcacatcgag ccgcgccacc
    16681 gcccggtaca acttcggctg atccatcaag cgctcgacca gcttcgtcgc cgcgcccagg
    16741 cagaggatca gcacgccctc ggccgcgggg tcaagcgtgc ccgcgtgccc gctcttgcgc
    16801 acgcccgtaa gcttgcgaac tcggtatagc gctttcgcgc tggtaatgcc ggcaggctta
    16861 ttcaggataa tcaggccgtc catctctgca ttctaacgca tctctcaaca acggcttgcc
    16921 cagcccggac gcgcggccct atgctgtgcg ccggaaacaa aggtgccatg caatcacggc
    16981 catcgacatg ctgccggctc ttcttcgcgg gatgggcaat gatgttgctg acgctcaccg
    17041 gctgcttcgg ttcaggcggt tcgggcgggg ccggccccgc gcaggacacg gatagggcta
    17101 ccgccgggcg ccgtagcgac gccgcccgcc aataccccct cgacagcctt cccacggccg
    17161 tcattcaggt aaagggacag gatgtgcagg tctggctggc gcaggaattc gacgcccaac
    17221 ggccgcacgt cgtggaagaa gggctgatgc atgtcccgcc cgaagagatt ggcgacgcgc
    17281 agggcatgct gttcgtcttc tcggaggagc ggctccgcgg attctggatg ctgaacacca
    17341 tcacgccgct ggacatcgcc ttcgcccgct gggacggcac ggtcgtcaaa acctggcaaa
    17401 tgccgccgcg caccctgcgg acgttttcgt cgatcgagcc ggccatgttc gcgctcgaga
    17461 tgaagcaggg cgcgtttgcg catctcggct tgcaggaggg cgaccgcctc accataccgg
    17521 acgctgtatt caagacgcag ccgtagcgcg tcgaaggggg attcggggcg cgtccgctcg
    17581 cacgctgcga ttctgcgcgt gccgcaggcc gccccgttca ggtgaggcga tctgctgcgc
    17641 atgtgcgacg taccgccgat cacggttttt gagaccaacg gcgcggactg taactctttg
    17701 caccagcgtc tcgccgcgcg cggcatcgct acgccccgag ttcccctgga ttgcgctgag
    17761 ggcgcgacgc tggacaatgt aaaaatcgcc gtcgtcgccc tgcgaaatag cgcccgggaa
    17821 gcggaactcc agcccgccgc tacactcctc gaacgcctcg tcaccgataa catccccaca
    17881 ctcgtctgcg gcgcggctga taacctgcgc tgggccggcg gcccgctggt cgactgggtc
    17941 gagcccgacg ccacgctcga tgagatcatc ggccgcctta acacgctcac gcgctatggc
    18001 ccgctcatca aacgcatgga gcgcgagctc gaccaactgc atcgcctggg cgagcaatta
    18061 aatcgctatt tcagcgaaat cgatcaggaa atgcgcctgg ccggtcgatt gcagcgcgat
    18121 tttctgccgg ccaccctgcc ggagacggcc ggcgcgaagc tcaccgccct ttatcggcct
    18181 gcaagctggg ttagcggcga tctctacgac gcgttccgca tagatgaaga ccacatcggg
    18241 ctgttcgtcg cagatgccat gggccatggc gtagccgccg gtctactcac gatgttcatg
    18301 cggcaagcgc tcgttcccaa gcgcgtcgcc gggcgctttt acgaaatcat ctcgccgcct
    18361 cgcacgctgg ctactctgca cgaatgcctg gtgcgccaga agctgccaaa ctgccaattc
    18421 gtgaccgccg tatacgccat cctgaacgtg cgcacccggg agttgcggct gtcgcgggcc
    18481 gggcatccct gcccgctaca cgtgcaggcc ggcggtgagg tcgcccaaat caacccggcc
    18541 ggcggcctgt tgggcctgcc agatttggac ggcgagtttg aagaggcgcg catcatcctg
    18601 gccccgggcg aaaaggtcgt gatgttcact gacggcctgg aacccgattt cttcgccgcc
    18661 acggaacaac gcggagcaga agctgagctc tcaaccgcga tgcgaagctg gctgtcactg
    18721 ccaatcgaag aattcaccga ggcggttagc gcccacctcg accttaaaga aggctcgctc
    18781 tacccgccgg acgatgtgac catgctcgcg ctccaaatcg atccatagca gagccggggc
    18841 gtatcgctcc aaattccgac ccgtagcaga gccgtggcgt gtcgccccgg attgccgcca
    18901 aaaacattcg ctttacagaa gtgccacccc cctaccgctt tcgaagtctg acaagcaccg
    18961 cctaactacc ctcggcaccc tcctccgcca atcccgcccg agccgcctcc accaattccg
    19021 cgaattgctc cagccactgc ccaaaatcct ctgcctgctg cgcccgaaac ccgcgctcca
    19081 tcgccgccgc cagcgcgcta agttccggat aaccatatga gccagtcgcc cccttcaggc
    19141 ggtgagccag catcgtcagg tgcccccagt ccaactgctc gaatgcctcg gcgatttctt
    19201 ccatgcgacc gggcagacgg cgtacgaaat cggcgacaac gtcacgcaat tccggatcat
    19261 cctgcagcag ttgcgaatag agttttctcg gctgattggc ggtcatgaag tcctccgcga
    19321 taaccgtcta tttcgcgtgc acaacgcccg ccgtttcgcg atgctcttaa ccccatcgac
    19381 cgtcccgccc tgtgcattga aatctccgcc cggaccccgt aggcttccgc acgatcgagc
    19441 ctgacccgcg tacactcaag agacctgccc cggcgaaaca gccgaaaacg ccattaggtc
    19501 catccgggtc ccggaagctc tgcggaggcg cgaaatgtgc gggattgtgg cctacatcgg
    19561 gcgacgtgaa gcttgcccca ttctcttgga aggcctgaag cgtcttgaat atcgcggcta
    19621 cgactccgcc ggtatggtcg tgatccgcga caatcgtctg gaaatgcgcc gcgccgtcgg
    19681 ccgcatttcc gccctcgaag ccaaaattca ctgcgagctg gacggctcaa cgatcggcat
    19741 ggcccatacc cgctgggcca cacacggcgc tcccactgag cgaaacgccc atccgcattg
    19801 cgacgcatcg ggccgtttag ctctgattca caatggcatt attgaaaatt atcgggccct
    19861 caccgcctac ctcaaaaccg agggcatcga actggaaagc gaaaccgaca ccgaggtgct
    19921 cgccaagctc atcggccacc tctaccaagg cgacctggcc gatgccgttc gcaaagcgct
    19981 gaacgaggtc cgcggcacct tcggcatttg cgtcattcac gccgaccacc ccgacatgat
    20041 cgtcggcgcc cgtcgcggca gtccgctgat agttggtatc ggcaacgacg agttcatcat
    20101 cgcctccgac gcttccgcca tcgttcagca cacgccgcag gtcatctatc ttgaagacaa
    20161 cgaaattgcc gtggtccgtc gcgacggcac cttccgcacc atgacgctcg acgcggctcc
    20221 ggtcaccaag gaagtctcgg cgatcgagtg gaccctgacg caactcgagc tgaacggcca
    20281 cgaacatttc atggccaagg aaatcttcga gcagcccgaa tccctgcgta attgcctgcg
    20341 cggccgcatc gacaccgccg aaggccgcgt cgttcttggc ggtttgaacg gcgtcgcccg
    20401 cgaactggtg cgcgccaaac gcatcatcct cacggcctgc ggcacggcct ggcacgcggc
    20461 tctcgtcggc gagtacctct ttgaagagct ggcccgcatc ccctgcgaag ttgaatacgg
    20521 cagcgagttt cgctatcgca accccatttt gacggacaac acggtcgtga tcgcgatttc
    20581 ccaaagcggc gaaaccgcgg atacgctcgc ggccgtgcgc gaggctcgcg agcgtggcgc
    20641 cttggccctg ggaatcgtga atgcggtcgg ctcaaccatc gcccgcgaaa cggatgccgg
    20701 ggtgtatctg cacgtagggc ctgaaatcgg cgtcgcttcc accaaggctt tcaccggcca
    20761 gcttgccgtc ctctcgatga tggccgccta cctggcccgc cgccgccaca tgggtcaccg
    20821 cgaatgccag gattatttga tcgccctaaa cgaaattccg gccgcgatcg agcgcacgct
    20881 gacgctatcg aaccagacgc gcgaaatcgc cgcccgttat gctaaagcca acaactggct
    20941 ctacctgggc cgcggtcttc agtacccggt cgccctcgaa ggcgccctga agctgaaaga
    21001 aatcagctac gttcacgccg agggcctgcc ggccgccgaa atgaagcacg gccccatcgc
    21061 cctgatcacc gaggacatgc cggtggtggt gatcgctccg cgcgaccact tgtacgaaaa
    21121 ggtcgtcagc aacatagaag aggtgcgcag ccgcggcggc cgcgtcattg ccgtggccac
    21181 cgaaggcgat gacctgatgc cgcgcttggc ggacgacgtc ctctacgtcc cgaaggtccc
    21241 cgaaccgctg caaccgttcg taaccgtgat tccgctgcaa ttgcttgcgt accatgcagc
    21301 cgtgcggcgc ggctgtgacg tggacaaacc gcgcaacttg gccaagagcg tgacggtgga
    21361 atgagcatag gagagtcgtt gctgctgcaa agcggctctc tcgctccctc cgcgcgcctc
    21421 ctggcgaggc gcccgtttcc atccagcggt cgcgagggta gactgacgcc ctacatgaga
    21481 cgaggcacgg aacacgagta agtgtgatgg cagacacgat ttccgcccgg ctggaagcgg
    21541 ccccacataa gtttgcaatc gtggtgagcc gctggaacga attcatcact tcgcgcctgc
    21601 tcgacggggc gatcgacgcc ctggtccggc atggcgcgga acctggcaat ctgctgcggg
    21661 tctgggttcc cggggcgtgg gagataccgc tcgccgcgca gcggctggcc ggaaccggcg
    21721 tatatgccgc catcctgtgc ctgggttgcg tgatccgcgg gcagacgccg catttcgagt
    21781 acgtcgcggc tgaggtcgcc aagggcatcg cgcaggtgtc gctgagcacg ggcgtgccga
    21841 tcactttcgg catcatcacg gccgacaacc tggagcaggc catcgaccgt gctggaacca
    21901 aggccggcaa caagggcgcg gacgccgcac tggccgcgat cgagatggcc aacctgctgc
    21961 cgcaactgtc atcgctgaaa tgagtgttgg tcgctttcgg gtgcgcagcc taaaaccgac
    22021 cgaatcgcgt gtccctccac ggcgcaaacg ccaggaaatc gctgtgcgga tgaccggtaa
    22081 accagggtca gggcggccgc acgggacgga atacaatccg ctattgacca ttattttggc
    22141 tgatcaagct agcatttatg tccatgccgc gccgcccgcg tcctaaacac acgcaacgtt
    22201 cacatgcccg cgctttggcc cttcaggcgt tgtgcttgtt cgattccctg ggcgagggtt
    22261 ttaacgaaca actcgagtct ttcctgcatg actcgcagac ttacgccgac ttgggtatcg
    22321 cccgctcgcc aggggtgagc aatctgtgct ttgcgcgcga tctggcccgc ggcgcgtggc
    22381 agagtcgcga ccgttacgat cagctcctga ggcaagccgt cgccgactgg agcctctccc
    22441 gcatgcctcc ggtcgaccgc aatattctgc gcatggggct gcacgaactg ctcgcgcaac
    22501 cggacacgcc gcccgaagtg gttatcaatg aagcggtcga actggcccgc gagtttggca
    22561 gcgaagactc cccggccttc gtgaacggcg tgctggaccg aattcgcaag gagctgggga
    22621 tcggggacag cgccggatag cgcgcgagcc ttgcagccag cgcagcggcc ggcttctact
    22681 cggccgctac ggaccggcgt tttccggctg tgtttgggcg gcttgcgtgg ccggcgccga
    22741 cgccggtgct gatgccggcg cagaagctgg aggcgcttgc gcttgagcca acagggcgct
    22801 catatcgttc gcgatttctt cggagaaggc ggcgggaggg tgttggcgca ggctggagag
    22861 catttgaatg acttgctggg cgccgcccgc gttcaggcgc gccgccagct ccggacgaac
    22921 cgctgcccac agcgcgccgc cgtccagggc cgggctgccc ttagccaagg ccgacgccag
    22981 tgactcgttg taccgctcgt tgagcaacga tatccgcagc acagcctgcg ctgcttcagt
    23041 cagttcgggc gcttggctca tccgcaaatc gcgcactgcg gccaggtagg cggcgatggc
    23101 cgcgtcgatt ttgttctgcg ccgcgcgctg cgcgccaaca cgggtccaga cgcgtcccag
    23161 ctcacgcaag ccctccggaa ccttcgccaa cgaggcctcc agcaccgcca gcaattcatc
    23221 aacctgtgcg tcgcgttctt cggtatccgg cagccgcccg agccagcctt ccacctccgc
    23281 caccgatcgc ccggatagca acttcacggc cgcctgccag gagatccgtc gaatggtcgc
    23341 atccggctcg ctgctcggcg ccaggcgttc ccagagggcc gccaagtgct cgtccgtgca
    23401 ggcgcagtcc gccagcgatt cgaccgcggt gcggcgtacg gtcaggtccg cgtcgcgcgt
    23461 aaccggaatc aaagcttcaa tttgtgcttt tacggccgcg atcccgcggg ccgccgcctg
    23521 tcgcacgagc ggcgcttcac gctcgcacaa ggcctgtgaa aacgcggtag cgaaggccgg
    23581 gtcagcgatt tgcgccatgg cccacaatac gcgttcccgc agtgccgcct gcgccggcgg
    23641 cgtctctgcg aataacttca gtagttcatc tgcaatcgcg tcacgctccg ccgcccccag
    23701 cacgccacgc tccgcttgcc gccccagtgc cgcaaccgct tcggcacgcg ccggattgtc
    23761 ctgcgaacgc acgatattga ggagcgtctc gcacgcttcg ccactcccaa cgtagcccag
    23821 cccgttcacg agagcctggc agacattcgc ccggcgttca actgccaacg cgctcaggaa
    23881 ccgctccgca tccgctgcat cgcggaaccg ggccaccgtc tgaaccgcgg cgacgcgcac
    23941 ggccggatcg gcatcgctca gcagttcacg ggtcaattcg cgcacgggcg ccgccgcgtc
    24001 cgccggcagg ggtttgccct ctccaagctg ccgctgaacg agctccaatc caagcagccg
    24061 aaccaacgcg gatgggtctt ttaagtaact ctgcagcagt ggcccgcgct ccgcttccgg
    24121 agtgcggatg taatcggcgc ggtaagactc aaccaggcgc agctccaact cacgcaagtc
    24181 ctgctccagc tcacgcgatc tgcgcaccag ccgatcaatc tgaccttgtt gccagtcagg
    24241 caacggctca ttctggcttt gctcccacca cgccagggcc cgcccggcat cgcctccgaa
    24301 atccatggcc gtgctgcgct cgagtgcttc cagagcgggc tgcgcgatcg aagagtcggg
    24361 cagcgcgacg gcctcgacca aagccgccac ggcggagcgc ttatttatcg cccctagcgc
    24421 ctcaacggca gccaaacgca gcaccgccgg ctgcgaaacg tccagcatca tgtcccgcaa
    24481 gcgcgccgag acgccgctat cgcgatagcc cgccaaagcc gcggccgcag cgtgacgcaa
    24541 atcggcatcc gggtcggcca acatcgagat gagcggctcg atgtaagcgt catggggatc
    24601 cggaacatct gccagcgcca gggcgacggc caagcgtgcc ggcttgtttt cgctcgccaa
    24661 aacgccggca agacgattga cggtttcggg ccagccaagc cggagcaatt ctcgcgcacc
    24721 ggttcggcgc gcagccaacg gcaagtttgg cccctggatc agcatgagga agtcttcgag
    24781 acgttgctga tcggcaattc ttgtagcggg agcggtagcg ggtgcggtag cgggctgagt
    24841 ttcgggctcg ggaggcgcgg cgtaagctgg aaaaaggggc attagaatag cacttagaag
    24901 gactccggtc ttcccaagct gctttcctgc cattcaatcc acctgctgcc gccgctgatc
    24961 ggagcgactg gaagtctgat aaatccagtt cccccagcat taatctcgcc gcacattata
    25021 cacattgttg gacaacaggc gacgccttgg gtttgcaaat ctacatatcg gagcttgttc
    25081 gtttagaccg gcgagggact caccgatgaa aaccgttgac gccgcgaggc tttgtgctac
    25141 gatccgcgcg gcgcggccgc tggaccagcc ggtcaggcac ggagcgccag ccgaagcgtg
    25201 gacaggaaga gccagcggcg gccgaatccg ccggaggaag tccgatgaag cgagcggcat
    25261 gctgcgcggc catgatcgcg tgcgtgtggg cggcgacgcc ggtttgggcc gggctgtacc
    25321 aagacattta ccagggtctc tacatcctat ctacaccaag cgggtttccg ttattgaccg
    25381 cgtcggacgg cacgcgcatc aacggccagc gcagcgggcg cctgcagatc cagcgcaaca
    25441 gccccggccg gggctacgat gtgcagttca atcgcgtctt tggcttggac tcgcaggggc
    25501 ggccggaagt tttggacctg ggcaacttcg aaatcgagtt acagggcgcc acgcaagcta
    25561 ccttcggata caccaaccgc ggtccgctga tcggcaccgc caatttcgcc gcgaacaacc
    25621 tgaactattc gctgcgcgcc aagagcggca ttcagaactt tacgctcgcg ggaacgttga
    25681 atatcgcgga ggccgtggaa atcaaccagt tcggcttcta cgaagcatcg atcaacatca
    25741 gcaacaccaa ttcggagctg tttctcgacg ggttggccca ggagagcgtg cccgagaact
    25801 tcgacatcgg cccgatcaac gtcaagggca atatcttctt tgacgccttc gtggcgttgc
    25861 tgtcgagctt gggcgttgat accaccgagt tgcaagcgct gacccccgga tcagccattg
    25921 ggcagattgt tgatgagatc gcactgcagg tgccgcagtt ggtggcgggc atccaggtca
    25981 gcgaggacgg ccagttcgcg ccgccgccgc tggtgctcga cgctgccgcc gacatagcaa
    26041 cgcccggcct ggcgcccgca caaaccgcac cgggcttgac gatcccggag cccggcacat
    26101 tcctgatctt gggcttgggc gctgttctgt ggggctggag acggcgctag ggcggcccac
    26161 agggcgtagt ccaatggccg tgggcggaag caagctttgc ccgataaccg gcatcgtcgc
    26221 cgtccctgcg caaggctccg ataaatagtc cagcttcggg cgccggagtg atatttagct
    26281 tggccggctg tcgcccgcct gttatactaa gacctaacgt tgtactggct ttcggatgcg
    26341 aagctggtag gggataggag cttacttgtc acttacaaaa ggaggacttg gcttgaagta
    26401 ctgttggcat ctcttgtttg tggggctcct cttggcttgc cccggcgtgg cccgcgccta
    26461 tgagacgggc gacttgaact gcgacggcgc ggtaaacgtg tttgacatcg atccgttcgt
    26521 tctggcttta acggatccag tcgaatatgc cgcgctgttt ccgtcgtgtg cttatctgtt
    26581 ggcggacgcc aaccacgacg gcgaagtcaa cgtgttcgat attgacccgt tcgtgcagct
    26641 gttgacaagc gcaccgccga ccgccgcctg ctgcaacccc gctggcggct gcgcggttac
    26701 aaccgaaagc ggctgcgacg gcgtctggca tccggagtgg gcggattgca cagtggctga
    26761 ttgcccaacg cccgagccgc cgatcgccat ggaattggcc ggaaatccgc tgacggctta
    26821 tccgtacttc gagtacgtgc gggccttcca cgtcaacgcc ccgatccaaa tggcgatcga
    26881 tcccacgcta cgcccggaga tcgttggccg gagcgccgat gtttacatcg tcgagaagaa
    26941 gtccgcgggc gagtgggcag ttgatccggc tttggtcgat gaaacggccg gtgggccaac
    27001 taccgtgacg ttcggcggca ccacgattca ggaaaacacg ttccagatca ctgggccgaa
    27061 cgagctgagc gcggcggtgt ttcaagcggc caccggcgcg aacaccggtc tgggacgtgg
    27121 ctatgacatg atcgtcgaca tgaaccagaa cggcgtgctg gacggcggcg actacatcga
    27181 cggcctgggg cgggaagccg ggttgtacgt atgccacgac accaccacgg ccggcccgtt
    27241 ggccgtgacg gagacgcttt acaacgtcgg cacgatcttc ggcattcctg ccaccgttgc
    27301 cggccaggat ctctactacc cgacgaacat tgccagcatg ggccaactgc cgctgatcgt
    27361 gatcagccac ggcaacgggc ataattacca gtggtacgat cacatcggct tccacatggc
    27421 ctcgtacggc ttcatcgtca tgtcgcacgg caataacacc ggcccgagtt ccgggctata
    27481 tgcttcgctg accacctgcg ggcacaccga cgccttcttc cacctgttac cgtccatcgc
    27541 cagcggcgca ctcgtgggcc acgttgactc gcaccggatc atctgggtgg gacacagccg
    27601 cggcggcgag ggcgtggcgt tcgcctaccg ccgcatcagc gcgccgggac ccgacgccta
    27661 cactccgacc tactacaccg ccgacgacat catcctggtt gatagcatgt tgccggtcga
    27721 cttcttcggg cccaatcgca ccaacccggg cgcggcgaac tatcacctgt ggacggcctc
    27781 gggcgatagc gacgtggatg gctccgccgg ttgcgacctg tgccagacgt tccacataca
    27841 cgaccgggcc acgcgcttcc gcggctcgac tgtcgtgcag ggcaccgggc acggctggtt
    27901 ccatgactcc gaaggctccg gggcctggtt ctcgggtccc tgcccgatca acgagtccgg
    27961 cacgcacctg gtgcagctcg gcataatgtt gccgatgtac aagtactacg ccgaaggcaa
    28021 cgtcccgggc gaggactacc tctggcgcca gtatgagcgc ttccatccgc cgtctgtgcc
    28081 cgtgggcatt gatccgtgct acgtcgtctc gcaggaatac cgcaacatgg gcgacgaggg
    28141 atatgctttc attgatgact acgagacgca gcccggcgcg ggcacgagca gctccggcgg
    28201 cgcggtcacg tacaatgtca ccaacctgac tgaaggccgg ctggatgaca acaatggcag
    28261 ctttgcctgg agctccggcg atccgttcaa cggcgccacg caggccagca gtgactacag
    28321 tgataccagc aagggcgtcg tctttgactg gaacggcgca aataggtact acgaatgggc
    28381 cgtcgttccc acgctgcgca acttcgccga ctggaagtac gtaagcctgc gcggcgccca
    28441 gggcacgcag cacccgtaca cccaggcgac caacggcatc ctgacgttca ccatcacgct
    28501 gcgcgacagc gccggcaata ccagctcgat cagcaccggt gcctatggcg gcggcttcgg
    28561 aatgccgtat gcccgaagcg gcggctggca taacgaaatg cgccgcatcc gcattcgcac
    28621 cgcggacttc ctggccaacg gcagcggaat tgacctggag aacatcgtgg cggtgcgtct
    28681 ggacctcggc ccggattggg gcacgccgca gggccgcatc gtgattgacg agctcatgct
    28741 cgacaacgac ctgccgccgt tcttcatccc cctgaccatg tacgtggcgg agcctccgcc
    28801 ggagttcatt ccgccgcacg ctccgtaccc cgtgaacgtg gaaatcctcc cgggcaacga
    28861 tacgttggtc cccggatcgg ccatgatgta ctaccgttat gacggcggcg actggcagtc
    28921 gcagccgctg gtgaacgtgg ccggcgactt gtggcgcggc acgctgccgg cgccgcagtg
    28981 cggcgatcgc cccgagtact actttgaggc ggaaggcgcg gtgaccggcc tgctgtctgc
    29041 gccgctgggc ggtgcggcag ccccgtattc ctcgctggtc ggcacgtaca taagccttct
    29101 gcaggaagac ttcgagacgg cggccggctg gaccgtgtac aacacgccgg gcatgacctc
    29161 cggaatgtgg cagcgcgcga ttccgggcgg ccatgccgac cactcgcccc cggaggacta
    29221 cgacggctcc gggcagtgct acgtcaccga caaccgcagt accttcgatg tggatggcgg
    29281 tcctacccag ttgatctcac cgctgctcga tctcagtggc atggaagatc cggtggtccg
    29341 cttcgcggat tgggtctact gcgatgatgc gacgggaatg tacccgtcac agcaagatta
    29401 cctcgacgta tatgtttcga gcgacgacgg cgcgacctgg gtgcaggcgg cgcatatcgc
    29461 caaccacgac aactggatgg tgcacgagtt ccatgtcgtg gacttcatcc ccctgaccgc
    29521 caccgtcagg gtcagcttct ggtcccagga caacccgaac aactcgatga ccgagatggg
    29581 cgttgatgcc gttagcatct tcgacgtgca gtgtgactaa actacttgca gaaatgaact
    29641 cttgcttctt gcagcgaaag gataggaagg aaatgaaagc aatactcgcg agtgtgtgtc
    29701 tgttgacgat ggtttcgatg gtcttcgccg caccgcccgt cgccaagact cccgcggcgg
    29761 tggacgaagt cgtttatgcc cgtcccttca ccttgagcga aagcttccgt tacgactggt
    29821 gcaatgcgcc gttccaggtc agcgaaggca cgatcctggt tctcaaggtg cagaccaacc
    29881 tggttatccc gcgccagatc gaggagccgg tcctgtacgt cggtaatcag gccgccatgc
    29941 gcctcaacca gggcgataag tcgggccatc tgatcgtggt cgtcccgggc gaagtggatc
    30001 tgaccaagga cctggtctgg ttcggcacgc cggaactccc cggccgcgtc acccccggcc
    30061 aaatcgaagc tcagcggcgg ctggctgacc aggccggtat caagcccctg tcggccgaga
    30121 aggctaaggt cgcgaccgac aacggcggcc cgcgcgtcaa ggcgaaggac atgaccaccc
    30181 tgttgcgcga cgtcatcgcc ggactggtcg accaatactc gccgcaggaa aaagtcctgt
    30241 ccgagacttg gcggatggaa cccctcagca attagtcgtg aacgacccag cccgcgcgct
    30301 tggccaaaat agccggcgcg caggctaacg cgagatgaac accaggcggc cgggacgccc
    30361 tgtcccggcc gccatcgtat gcgctctatc cccccggccc gatgaccttg agtgcgtcaa
    30421 ccaattccgt ttcgcagacg ctgtaccccg gacgcggccg gcctatcccc tccagcagga
    30481 cgaatcgaac ggtacccgcg cgagctttct tgtccaagcg gcacgtagcg gcgatttcag
    30541 ccccatcgac acgccgaggc aaatgtgtcg gcagcccaaa cctggcgatc agtgaggtga
    30601 tgcgctccgc gcgggctcgg gccaggcctg cctgacgaca gctcaactcg cccgccacaa
    30661 tcatgccgag acccacgcat tcgccgtggc gcagctcgta gccgagcagg tgctcgattg
    30721 cgtgccccgc cgtgtgccca tagttgagga cggcgcgcag cccgtgctcg cgctcgtcct
    30781 gcgcgacgat tgccgccttt attcggcaat tccacgcgat cagttcctcc agcaccgccg
    30841 gatcgcgagc caggatggac tgcgcctggc gttcctgcca ttccagaaac gcttcgccgt
    30901 cgatcgccgc atgcttcacg ctctccgcca atcccgcgat gaagtcacgt tgcggcaggg
    30961 tgtgcaggaa atcggtgtcg atgaacacgg ctgccggctg gtgaaagacc cccaccaggt
    31021 tcttgcccgc cgccagattg agcgcggttt tgccgcccac cgcggcatcg atggcggcct
    31081 ccagcgttgt gggaacctgg atgaagcgga tgccccggtg ccaggtggcg gccacaaagc
    31141 cgcccaagtc acccaccact cccccgccca aagtcacgat caggtccgag cgttcgacgc
    31201 cgccccgcgc cagcgcctgg tagagcgacg cggctacatc cagggttttg ctcgcctcgc
    31261 ctggggcaac ggtcagaacg agggggcacg ttgagcgatc ggcgtgtcgc aggacttcaa
    31321 gaaccggctc aagatgcagt tcagcaaccg ttttgtccgt aataatcgtg atccgaggtt
    31381 tatgggccgg cggaaacctg ccacatgggg cgttcgccag ttccgatagc gcgctgcgcg
    31441 cgccggcccc gatgtggacc ctataaccgc cacctgccag ctccacgttg agggtcttca
    31501 tctgccgacg gtcctttcgg gcccgttttg gtcgcacgcg cccaggtgtt ccgccctacc
    31561 gtctaactac cgagccagct accggttacc gggagcactc ccgccctaca gttgcttaca
    31621 ttaacgtaag tgccgatggc cactgtactt acgacaagcc tgacggacgc ccgtcatcgc
    31681 acagttattg acgacggcac gaattattgt taacataaag ccctatggca cgactagcta
    31741 ccaagcgcca gctgccgggg aagcgactca gggcccggcc agcggctccc ccaaagtccc
    31801 gtcggcccag ccgttccgga tcgatccgta cttaccgcgc cgccctggag ttcgtcaact
    31861 cccgatacaa cttcgagaaa tccgcgcggc ccaactatgc tccggtcaaa ttcaaccttt
    31921 cgcggatgtt tcggctgttg aaggagcttg gtaacccgca ggacaagatc aaggcgatcc
    31981 acgtggccgg cactaagggc aagggctcga cctgccacat gattgcggcc atgctgcaga
    32041 acgctggtta tcgggtgggc ctgtacacct cgccgcattt tacggacctt cgcgagcgga
    32101 tctgcgtcga cggcgagcgc atcagcgagc gggacttcac gcgcatcatc gccaagatcg
    32161 ccccggtcgt gcggcgcctc gagcgcgatg agccgacgtt tttcgaagtc gtcacggccg
    32221 ccgccttcct gcactacgtg aacaagaagg ttgatttggc ggtaatcgag accggcctgg
    32281 gcggccggct ggattcgacc aacgtcgtca agccgctggt ctgtgcgatc acgagcatca
    32341 gtcacgacca cgtggcacag cttggcaaca cgctggaaaa gatcgccgag gaaaaggccg
    32401 gaatattcaa gcccggtgtg ccggttatca ccacccgcca gccggcgggc gtgaagaagg
    32461 tgctgcgcgc ggccgccgag gacaagaagt gcgacctgcg catcgtcggc gaggacctgg
    32521 aattcagcta ccggtttgaa tgctcgcgcg ccaccggacc gcacattcgc gtgtgcatgt
    32581 cgacgcccac cagccgcttc gaccatttgc aggtgccctt gctgggcgag catcaggccc
    32641 ataactgcgg catcgccctc ggcgtgatcg atgccctgcg gcagcgcggg tacgtcattc
    32701 ccgagcaggc ggcgattgac gggctggcga aggtgcacat cgaaggccgg ctggagatca
    32761 tccgcgacaa tccgcggacc gtggtggacg tcgcacacaa cgcctccagc atcgccgccc
    32821 tgatgcgcgg catcggccag aacatcacct atgactcgat ggtggtgatc ttcggctgcg
    32881 cttcggacaa ggatatcgac ggcatgctgg gccaggtgca gctcggcgcc gacaaggtga
    32941 ttttcaccaa catcgattcg ccgcgctcgg ctgacgcccg ggaattgcac gcgcgcttca
    33001 ccgagaagtg cttcaagatg gcgcaggtgg cggacaacct cgaagaggcg taccgcatcg
    33061 cacgcagttg cgtgacgcgc gaggacttga tctgcatcac cggttcgttc ctgctggtgg
    33121 gcgaggccaa gcgtctgctg gcacagggca agctggacta agttcttagt tgttagctcg
    33181 gcatgggcga gttcttgctt agagctgctc taggcgcagc ttcagatcgc ggtcgtgcag
    33241 ctccgcccga aactcgtcga actcgccgtt gaccagaatt ttctgcaggc cgaacaggtc
    33301 cttattgatg cgatgatcgg tgcagcggtc ttgggggaag ttgtaggtcc gaatccgatc
    33361 gctgcggtca ccgctgccca ccattgagcg ccggtcttgc acgatctgtg cgcgttgttg
    33421 cgaggcatag tgctcgtaga cgcgcgacat caacacgcgc cgcgctttgg cgcggttctt
    33481 gtgctggctc ttttcatcgc gcatcgaaac cgtgattccg gtcggcttgt gctccagccg
    33541 aatagcgctg gaaaccttgt tcacgttctg cccgccgggc ccgcccgcac ggctcacgta
    33601 ttcgataacg tccttctccc agtcgatgtc cacctcgacc tcttccggct ccgggaggac
    33661 tgcgaccgtc gcggcagacg tgtgaatccg cccctgggcc tcggtcttgg gcacgcgctg
    33721 cacgcgatgc ccgccggcct cgaagcggta gtgcatgtaa acctcttccc cggtgatgtt
    33781 tacgatggct tccttcacgc cgccgaggtc ggagtccgac agagacatca cctcggtctt
    33841 gaaacccttg cgggccgcgt aggccaggta catgtccagc agctcgcggg cgaacaaagc
    33901 cgcctcctcg ccgccggtgc cggcccggac ttccaggatg atcgagcgga tcgaggcctc
    33961 gttgccggta acgagccgct cctgcatctg cgtaagcagc cgttcgcggc gcgccagcag
    34021 gccgggcatc tgctcttccg cgagctgccg cagatcgtcg tcagagccgg gctcgtcgac
    34081 gatctgctgc gattcttcga tttcccgcat gagcccgcga tagtcgcggt agaggcgcgc
    34141 gatcttctcg agctcaccgt gctcgcgggc caggcgcacc atcatcgcgc cgttcgaggc
    34201 cacctcgggc ttgttcatct ccgccgccaa ttcgtcggcg cggcgcgtca tctcttccac
    34261 gcgcgccatc cacggcggat cagcaatctt tgtaaccggc attattagct ctcagctatc
    34321 aggctgctat cagctctcaa tatcggccgc ttgccgctgc cccgttcgcc tgtgcggcct
    34381 ggccgggaca tataccaaaa aagtacggcc cgcgggcggt gaaccggggc cgtaggcgaa
    34441 atatcgaggc ttaaggcagc tacttgacgc cgggcttctg ctccgtgtct ttcttgaagt
    34501 agttgccgcc gaactttttc tggaaccgtt cgacgcgacc ggccgtatcc acgaatttct
    34561 gcttgccggt gtagaacggg tggcactcgc tgcagatgtc gaccttgagt acgggcaccg
    34621 tggagcgcgt cttgaagctc gccccgcagg cacagctaac ctgcgtctca acgtaattgg
    34681 gatggatgtc tttcttcatc tcggtttcgc ctcggccaaa cgtgctcggc ccctgtggcg
    34741 gagcttctcg cacgagccct gtattatgtg cgacggcggt cgtaaaagca agccgagcag
    34801 cgagccgggt gccaagtccc gcgccgacgg ggcgcgggcg tgccttggcc agtctccggc
    34861 tactaactaa gccgccccgc ctgacgctgt tcaacattcc cccacgtttg ccgcctgcat
    34921 cgagcgtcga atcgccgcaa ccaggctctc caggtcgaac ggcttctcca gcacgctcac
    34981 attactggca gcgcgagctt tctccgccac cacggtcgcc ggcagcccag tcgtgaagat
    35041 gacgcgcgcc tccggcaact cgtcgcgcac cgccagcgcc acgtccaagc cggtcttgtc
    35101 cttcaacagc cagtccgaga tcagtaaatc cggccggaag tcgtgcgcca cctgaaccgc
    35161 ctcgtcggcg tccaaggcgg tgcgcacctc gtggccctcg ccggtcaaaa agtggtgcag
    35221 catccgacag gggatgggct cgtcatcgat gaccagtatc tttgccatgg cattcaccgc
    35281 ttccacttaa tataaggccg cgcaacggca gcgccgctga ctagcttagc cgattggaat
    35341 aaattctctg ttatgagggc gtaggacccg gcctagcccg cggcgccaga ttaggtcgcg
    35401 ccgttgtagc cttcttccag agcgcgcagg gttttgcgga cggccatgcg gtcggccggc
    35461 cccatgcggt cgatgatgag cacgccgtcg aggtggtcgg tttcgtgctg ccagatgcgg
    35521 gccagcaggt cctccccctc aagttccagc ggattgcctg caaggtcaag agcctccagg
    35581 cggcagcgct tggcgcggcg aactcgaacg ttcaccccag gcagcgacaa gcagccttct
    35641 tcggcctcaa cattaccctc gcggtcgcga atgacgcagt tcaccagggc catagcgtcg
    35701 gcggcctggc cggtggggct gaccacgatc agacgtttga gcacgccgac ttgaggagca
    35761 gccaaaccga cgcccttgtt ctggaccatg agctcaatca tgcggtcggc cagcgcggcc
    35821 aacgactcat cgaaaacggc taccggctca cagggctgcc gcaagcgcgg atctgggtag
    35881 tggacgaccg ccagttttcc ggggtccagt ttcgtggtct gaggcatacg gcctatcccc
    35941 tgttaatcgt gaattcgcca gtctcaaaga gcaaatcggc cggaaaggcc ctcagccagc
    36001 ttccccgggc tttttgcttt tatagtcacg acggcattct gtgagcgctg ctctcctatc
    36061 gcataagcct tggattatag tcggaatcag aaagtctgtc cgagcaggaa cgcacgggcc
    36121 aagcgccc
//
LOCUS       DAOSYS010000006        35700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_970_length_35700_cov_5.375929, whole genome shotgun sequence.
ACCESSION   DAOSYS010000006 DAOSYS010000000
VERSION     DAOSYS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 35700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35700
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_35700_cov_5.375929"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /gene="dxs"
                     /locus_tag="PLU99_00795"
     CDS             complement(<1..1367)
                     /gene="dxs"
                     /locus_tag="PLU99_00795"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOO15618.1"
                     /translation="MGDRILDTIRSPEDLRQLSLEQLSQLAEEIRSRIIEVVCKRGGH
                     LASNLGVVELTLALHRVFDFSRDRLLWDVGHQAYVHKLVTGRNAAFDSLRQAGGICGF
                     PCAAESEYDLFDVGHAGTAIATAVGMARADALLGRDVRAVALVGDASIVNGTAFEGLN
                     QAGTLRRQFLVVLNDNSMGISKTQGALAKYLARFRVSSLYEEVKSQVSAALPKVPLVG
                     QSVKDALEYLKDSIKASVSPHQIFEQLGFKYVGPVDGHDLKHLLDILQLLKDVPRPVL
                     LHVHTIKGRGCTWASKDPCAFHSPGKLEVDGDSVTVVKSERKSWTAAFAEALKQVISE
                     DQRVHALTSAMPDGTGLAKVREVYPENVLDCGIAEGCTVDIAAGMAKSGLRPLVCVYS
                     TFLQRAFDHIFQEVALQGLPVGFCLDRAGLVGGDGAVHHGYLDIAYLRAFQNMVLMAP
                     ADEPEL"
     gene            complement(1402..2205)
                     /locus_tag="PLU99_00800"
     CDS             complement(1402..2205)
                     /locus_tag="PLU99_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010317896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO15619.1"
                     /translation="MEKVGPHSRLAEAVRYSMTLPGKRFRPVLTLESCRVCGGDEERA
                     VAAAVALECIHTFSLIHDDLPAMDDDELRRGKPCNHKVYGEACAILAGDWLAVQPFAL
                     LAAEYPPELAVALQRTLAVGTLAMIEGQAADVAGEQRPTDAQLLEYIHLHKTAKLIET
                     ACRLGALCAGASPAALEALGNYGRRLGLAFQIIDDLLDATGSTATLGKRAGKDAAAGK
                     QTYPAAHGLEASRAAARREYLAARDALQMFGPAADRLRQLAEYVIARDR"
     gene            2613..3629
                     /locus_tag="PLU99_00805"
     CDS             2613..3629
                     /locus_tag="PLU99_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HOO15620.1"
                     /translation="MNLKQERYSKFALVREMMDTVDVVASFDPGRTAQVAQEIKRVGR
                     LLLAGEGSSRIVPAKNAIRKALTWGLDLQVVTDGSRQAAHYDLANFAVFCASNSGRTK
                     EVILLARKLAEAGNPHRYGLSANRGTLLEEVVKHIFVLTCGWEQAVAATKSVVEQALF
                     YQSLLCHVAGGKMTPDLDGLAEKIRHVLTLPIDPQIVQKAMKATTIYFAGYNDGVAEE
                     LTLKTNEITRRKSDFLEGTYAIHGVEEVMDPSDVVFVIDPIQEEVEKFQEVLVKGVGL
                     TVIAIADRPTPFPTIQVPAAGELTPMVYLCTGWNLLVEIGLALGINLDKPERARKVGN
                     ELVV"
     gene            3934..6138
                     /locus_tag="PLU99_00810"
     CDS             3934..6138
                     /locus_tag="PLU99_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOO15621.1"
                     /translation="MREVKVLPLEEFGREFVPPQYRPAGQDEYYLRNQQVGRPKDYWR
                     HLRADEIETLVKNNNTCADWDHMLVADPFDPRLIKNSEFYGLVRIGRMERVILEHHDL
                     QKPVGISGSLIINCDIGDNCAINNVRHLAHFILGANVMLLNIDEMHTTNYAKFGNGIV
                     KDGEDEKVRVWLDLVNEASGRAVIPFDGMTTADAYLWARYREDAELQARLRQITENQF
                     DSRRGFYGTVGDACVIKNCRIIKDVKIGPCCYIKGANKLKNLTINSSAEEPTQIGEGV
                     ELVNGIIGLGCHVFYGCKAVRFVLCDNSNLKYGARLIHSVLGSNSTVSCCEILHNLVF
                     PAHEQHHNNSFLTAALVKGQSNVAAGATLGSNHNSRANDGEIEAGRGFWPGLCVTLKH
                     SSRFASFTLLSKGNYQAELNIPLPFALVNDNQAAQRLEVMPAFWWRYNMYALARTTWK
                     VRVRDTRKTKTQNIEFDSLAPDTIEEIFDALRLLEIWTARARLRADGKDAAEHSLEDL
                     AALGRELLLSSPQRTAGLEVLGENMEKSDRKVVIMKPREGYLAYREMLHYYAVKNLVA
                     YLSAHPQATLDTMGKDLAGPRQREWVNLGGQLMRGPDSARLISEIKAGKYRNWFEIHA
                     AYDKLWAEYPKHKQQHALATLLELLGVTKLTPEIWDAALTEAVRIQEFVCQQVYISRK
                     KDYDNPFRQATFRNAEEMRAVLGTPEENSFVRQTKKETEAFKALAEEMRKRV"
     gene            complement(6166..7686)
                     /gene="purB"
                     /locus_tag="PLU99_00815"
     CDS             complement(6166..7686)
                     /gene="purB"
                     /locus_tag="PLU99_00815"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOO15622.1"
                     /translation="MPRPSRPRRPSPPDPSPKRQRKPVTKPRSSIFKSKPTFEHPLAA
                     RYASREMVAIWSPQQRYSTWRRIWVAAAEAERELGLPITAGQIRAMQAAVDDIDFDAV
                     AEYERQTRHDVMAHIHAFGDAAPAARGIIHLGLTSMDVVDNADLLLMRAALDLLQRRL
                     VAAMRVLAPFCESNADLPTLGFTHLQPAQLTTVGKRATLWLDDLLRATQAIDRVRRNL
                     ACRGLRGATGTQASFLQLLGDRAKVRRLEQSFVRRLGFEHCYPVVGQTYSRQIDAEIL
                     AALGLLAGATHKLCNDIRILAMLKEIEEPFERRQVGSSAMPYKRNPMRCERATSLARY
                     LISLISSAYHTHAAQMLERTLDDSANKRLVVPEAFLTADALAILLNNIFDGLVVYPAM
                     IEAHLAAELPFIASEEILMAAVAGGGDRQQLHERLREHSQAAGEGVKQLGRPNDLLER
                     LKADPAFVHVKWSGLLNPQRYVGLAPQQTREYLKRILGPALKGLDLGPAPSAELHV"
     gene            complement(7688..8968)
                     /locus_tag="PLU99_00820"
     CDS             complement(7688..8968)
                     /locus_tag="PLU99_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOO15623.1"
                     /translation="MAAAKRGSPLDRRRACGDFVDMDPLALDPYSFQMSPPPPRHDPV
                     PGADFETLCAHFGEDRMAQRGAVAVPIYQASTFMFPDSAAWEQREAPDSPYYEYTRVG
                     NPTTAVLEAKIARLEHGNWALALGSGMGAIAAAINACVHAGAHVVASGTIYWPTRRYL
                     RDYLPRFGVETTFVDSCDPADFEAAIRPNTRLMYLESPTSGLFEMPDVAPIAAAARAR
                     GVVTVFDNSWATPCFQTPLDLGCDLVVHSATKFIGGHSDVVAGLVAGREDTLRRRVFR
                     ELELLGSVCDPFAAWLLIRGLRTLKVRMEQHQRSGLALARFLEQHPAVRRVYHPGLES
                     HPQYAIGRRQLRGYSGLFSFELHDQSREATNRFMDRTRLFTIGVSWGGYESLVIGGTF
                     ISKDPQQPAWIIRLHTGLEATEDLIADVKQALES"
     gene            complement(9003..9851)
                     /locus_tag="PLU99_00825"
     CDS             complement(9003..9851)
                     /locus_tag="PLU99_00825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOO15624.1"
                     /translation="MGLIIHVDGGARGNPGPAAAGVVIHTDDGQPIHEAGYFLGRQTN
                     NAAEYQALLRALMRAEKCGPQTLSIYSDSELLVRQVTGEYDVKSPKLQQLYEQAQLFL
                     LRAPRWTLRHIKREENARADELVNMALDRESDVIVFDADSTAAAGSSQNPGAGEAAGA
                     RAEGDPAKLPSWADPGARSAAEPNVPASAVEAHRVRVVLARASNIDICPAAASLPPEF
                     SITATLPAGLCIHAAHSILPTVLAMLNTEPQEFATIPTLTVRCNRPGCGAVFHLSPLR
                     SPNGKP"
     gene            complement(9867..10598)
                     /locus_tag="PLU99_00830"
     CDS             complement(9867..10598)
                     /locus_tag="PLU99_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOO15625.1"
                     /translation="MGTTLGALLELQDIELQLVDIRRQLAQKQRLVERQAAKLQAARD
                     RLAAEQERLRRTQIEIDTIDLDLKGRQAHVSRLREHLNTVRTNKEYAAVLADLNNEKA
                     DANRLESRELELMAEMDKQRQAVAAAEEVERAEADRLRDFQAQASGAMATFSDKLKEL
                     EARREESIAQLEGRTVNLFERLSERYEGEVLAKIERTHPRRDEFICSGCHMSISAEHF
                     NAAMVRDDVQSCPNCGRILYIDKRK"
     gene            10978..12372
                     /locus_tag="PLU99_00835"
     CDS             10978..12372
                     /locus_tag="PLU99_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO15626.1"
                     /translation="MTRLFEKYAGRATRCMLTGRWCGAFLAVGLLSAWAAIAWAAPPA
                     EHRQSGDLRRSPVVDVFERCREAVVNISTTRVVQMRSVSLFESIFDFAPRVRRHKVHS
                     IGSGVVIHSAGYVVTNAHVVAQASDVQVTFSDGKSETAEIVAVDPEHDLAVLKVSGEH
                     AFASVKLGHTGDILVGETVVAIGNPLGLQHTVTAGIVSALGRDLVFNDNVTYKNLIQT
                     DASINPGNSGGPLLNVNCELIGINTAIRGDAQNIGFAIPVDRLWETLPVMLDLERRER
                     VQVGLRVSGPRAEVEEVRPGSPAADAGLKVRDQILRVNGEPVRDGIDYYVHLLNAHPG
                     DNMNLAVKRGGRTLDFDVPIRVTPLPDGDSLARKLLGIKLTTIPSRVRRQYDLAGLTG
                     MMVEEVERNGPARKALEPGDIIRQIENMPTTSLKQVGLVLEQVVPGDAVDIELIRFER
                     DGVYLINISIPTRK"
     gene            12719..13174
                     /locus_tag="PLU99_00840"
     CDS             12719..13174
                     /locus_tag="PLU99_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15627.1"
                     /translation="MEELGLYEVRFIGPAFFEARNLCRETFDRMALRDHLRKLEHWEH
                     DDVGGLAVDLDWFWIRDFKELRLAEVLIDDELGGHADLRAVVFVPTEAYPYARDGKRV
                     IWVLSVLDKQDDQYTLAETANLWLRRKSVQRYYERESAGEYERLNDLTD"
     gene            13205..13717
                     /locus_tag="PLU99_00845"
     CDS             13205..13717
                     /locus_tag="PLU99_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO15628.1"
                     /translation="MKVDRKPMLSQEIPKEQLAVGAAFLRAFMECDDEIQKHVVQLAK
                     LAGSPEATDREKTMALYSIAEALRPVMHNGHLGISVDEADQIAVEDRPDGAAALARLQ
                     NQKKEFSERLAALMERKNISQAELAKAIGVSQPAISLLLARKCRPQRRTVEKLAEALN
                     VETRELWLEG"
     gene            complement(13891..16905)
                     /locus_tag="PLU99_00850"
     CDS             complement(13891..16905)
                     /locus_tag="PLU99_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOO15629.1"
                     /translation="MESGYAERLFFLDGELGARDIERVVELLIDPVTEEFSVRREGES
                     AVPPAKARTVEVHRRPGVMDPVAESTLAELRAEGVGVQAVRTAWRYVLFGEMTEAELR
                     SAAGRVLANDCIEEVVIGTAGVKPAPQPPVFEFVLRRVPIRELDDAGLNELSRSGHLF
                     LSREEMKAIQAHYRPLGRDPTDLELETLAQTWSEHCVHKTLKSEIVYRGEPLPGGAGA
                     SRAGVAFERRYENLLKDTIGRATAELMEAGRGPECLSVFKDNAGIIGFDEQHGVAFKV
                     ETHNHPSAIEPYGGAATGIGGVIRDVIGCGLGARPIANTDVFCVAPSNWPADKLPKGV
                     LHPRRILRGIVKGVADYGNRMGIPTVNGAINFDPRYLGNPLVYCGCVGLIPRDKIEKA
                     ARPGDHIVLIGGRTGRDGIHGATFSSAELTDTHADEFSHAVQIGNAITEKRFLDAVLR
                     ARDDARGCLYSAITDCGAGGLSSAVGEMAEHVGATVHLDKVPLKYAGLRYDEIWISEA
                     QERMVLAVPPEHLERFLEIMREEEVEASVIGVFGSEGYGGAPRLVVHYEGKIVGDLEM
                     EFLHRGVPKRSAVVEWHPPQSQSEPSQSGPAPERGENSAGACDDLGRMLLRELARPDV
                     AAKEWVIRQYDHEVQGGSVIKPLVGPGQGPGDAAVLRPVAGSRRGIALGCGLALELSD
                     VDPYWMAAAGIDEAVRNVVAVGGDPRQTAVLDNFCWGRADDPQQLGGLVRACQACYDV
                     AVAYGVPFISGKDSLNNEFALDAADVEPLLALLRGMSEGDDRDAVRLRGILPELEHRI
                     RQTGRLAIPGTLLISAISIVSDVTRCVTSDLKASGNELFLVGGVPQVGFDPAEAAGIH
                     VAVADAIAEGLVAACHDCSDGGWLTAVAEMAFAGGRGVGIADEALSPMRAQGASEGHA
                     IRARSASQGQTTRSAGEGNPNTSPFAGMCAGYVIETADAGKLGAMMSRRGVRILDLGM
                     VRRDDLLSVGGHSVPVSELRAAWAGSPACGTT"
     gene            complement(17036..17854)
                     /locus_tag="PLU99_00855"
     CDS             complement(17036..17854)
                     /locus_tag="PLU99_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOO15630.1"
                     /translation="MALQAAGFEGKLLALARDPSDLEAAIETGAVHSGATRPTDLPKD
                     VGLFCLATPLSVMPGMLRQLAPILDEAAIVTDVGSVKVPVVNAAGSTLSHPCRFVGAH
                     PMTGFNQRGVSFARADLFHGKTVILTPVSATAPEAVQCVRSMWELTGAHVTEMSPAAH
                     DETIARVSHLPHVVSALTLLLAARQEGLHVAAPGLIQMTHIASRDPDLWQDILTSNRE
                     QVRVAISQLVDDLKQLDTWLERHEDGNVHRLLARAVQIRNEWLAEKFQRPDWID"
     gene            18076..19149
                     /locus_tag="PLU99_00860"
     CDS             18076..19149
                     /locus_tag="PLU99_00860"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOO15631.1"
                     /translation="MSTQPSTKNGPQPYLLDFERPINVIEQELLRLEAAAADGGSDFS
                     AIEKKRAELNEALRQVYGKLTAWDHVLVSRHPRRPLLRDYLRLITEDFYELHGDKVYG
                     DDRAMVCGFARIARQRVMIIGHQKGRDTREKIEANFGCAHPEGYRKALKKMQLAAKFK
                     VPIVCLIDTPGAYPGIGAEERGIAHAIAVNLMELSRLPVPMVAAVIGEGGSGGALGIA
                     VADQVAVMEYAYYSVISPEGCAAILWKTGEQAPRAAEALRLTPGHLKELGLIDAVIPE
                     PLGAAHRDPVGAARNLEAWIIQALREVRRPRIETLLRRRYERLRKLGSAFTTKSGMEA
                     KTVRSRGRRLGASRHVAALDSPS"
     gene            19570..20130
                     /locus_tag="PLU99_00865"
     CDS             19570..20130
                     /locus_tag="PLU99_00865"
                     /inference="COORDINATES: protein motif:HMM:NF022389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2617 family protein"
                     /protein_id="HOO15632.1"
                     /translation="MNRSGTCEALVQAADLHVFLYDRPLHPELFRHYRDFRVAQGRYY
                     ADVWIVGLNHVVTVTSGQRSMTELLACESDVLPARGLVARFRLKGERDQEKHLPDGWL
                     HMISTQVETMDEPLYKSVHNDLLRHACKRGWFHAYEEWADGDLVPFSYIDHEERDAEF
                     HIHAFHAFPHERTLIKTQSIFEMPPA"
     gene            20337..>22311
                     /gene="uvrA"
                     /locus_tag="PLU99_00870"
     CDS             20337..>22311
                     /gene="uvrA"
                     /locus_tag="PLU99_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO15633.1"
                     /translation="MPSERDMVHVIGAREHNLRSVSLDLPKNRLICFTGVSGSGKSSL
                     AFDTLYAEGQRRYVESLSSYARQFMGELPKPDVDQITGLAPSISIQQKTSGWNPRSTV
                     GTITQVHDYIRVLMARAGTAHCTNCGRPITAQTREQLLARILELPEGTRFMLLAPKVR
                     AQKGEHKDLFAELIQQGFVRARVDGEVIDLSNPPPLDRYRRHDIEVVVDRLVMRPNLR
                     PRLAEALDTALEAGGGIAILATLHEDEGQPEGVGQPAPAGRARLAHMRSRKQTANDVL
                     LSSAYACTHCNLGFEPPNPQMFSFNSPQGMCPHCDGLGTRFDFDPELLIPDPSLSFLA
                     PCVAAFRHEPGRWRRHIYEGVARHLGFDLRTPWRDLPPAARNALLFGTGNTHITYEWR
                     GRYGTWKHGGPFEGVLAELHARHKKATSPLARRFYEQFMRQGVCPRCHGARLNPQALA
                     VRLTARPSDARKPIEFNINEICQLPIRKALEFFSTLVLDDVRRLIAAEPLKEISARLQ
                     FLLDVGLDYLTLDRAAPTLSGGESQRIRLASQIGCGLVGVLYVLDEPSIGLHPRDNRR
                     LLDSLKRLRDLGNTVIVVEHDRDTMLAADRIVDFGPGPGVRGGHVVAQGTLDDLMAAP
                     DSLTGAYLSGRQTIAVPQSRRPIKRNHSAKQSRP"
     assembly_gap    22312..22411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22412..22597
                     /locus_tag="PLU99_00875"
     CDS             <22412..22597
                     /locus_tag="PLU99_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15634.1"
                     /translation="PDTESLPLTNTFSAPHSVSARLDAVIGTLSKPGANTLITLSTFF
                     WRLRGGRFYRPTLIIFS"
     gene            23193..23657
                     /locus_tag="PLU99_00880"
     CDS             23193..23657
                     /locus_tag="PLU99_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15635.1"
                     /translation="MILAYLEPLPPDSPQLPVICAAADKAVQTRPDYADLRYFAAYAA
                     FRAGDLPHARELVDCATRLNPQFKAALILAGRICLQQNEHAAALTNLQQALAAGADYP
                     DVYVLLGDAWQARGDLVRAAKAYRQALCKNHRLTEARRKLGAISAGRNGGPV"
     gene            23654..25366
                     /locus_tag="PLU99_00885"
     CDS             23654..25366
                     /locus_tag="PLU99_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15636.1"
                     /translation="MSYLLETLGRGLLVELLSAFENQLPGFSEEPEPLRDRLAASPNS
                     ADLAVRFGVSCLRRRRLAEARQAFESALELPPPSAQPALGLACVYDELGQLDQVLRYL
                     AIAAGHDPNDPAIVFAIAFTEERRGDTDAAIANYKRALELCPRLRNAWERLAAIAIMR
                     GHWREATACYEKLAKLEPGDLDILLTLATLCLQTGRTADATERYQQALVIEPDFSDEP
                     LAETEDLLAEGRLQEAIETLEALVERYPHAAPFRVHLGDLYAKADRGADAIEQYRLAL
                     ENQPGLLEATVKLGTQHLRLERYVDAAQTFNRAVELNDRLMLAFVGLGVSQHANGSER
                     EALATFDLAASLEPSTTLLFAESARLHLKAERSGAAGGLPEYSDLDDEEEAGAADDAD
                     EQLLQVALQRHRQALLRNPQHADLHYRYGLLLRQMGDYDEAIRAFRNAVRINPNYAKA
                     LVKLGILLKETGQTDEAVEVFRRALALNKEYVDVHYQLGLLFAQRNRFELAVEEFEQA
                     VAGNGQNMVFQAQLALALQNIGMLDRAAATWRSICELSTVAGEPDKMLEHRTRILRQV
                     NNRE"
     gene            complement(25404..27161)
                     /locus_tag="PLU99_00890"
     CDS             complement(25404..27161)
                     /locus_tag="PLU99_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15637.1"
                     /translation="MSVKSHIVTTICAGCLAVLTFAAEPDLRLVCSPGELQAAQPDGK
                     ALWKIRLANDRGGATARWDGQVALLSNGLLIDATGRLLCMNAQFAPAEALAPGATDAW
                     PEPAWDEPLFEIAPPPPPDIADYHNTPLLDRAGNAWLILTHWEGTHCDLQSRKSVGHS
                     GDWGPVQTIHGGTSYILATEAVMDSDENITVAFREGGYHLKVLRYTPAGGWEPAQTLY
                     STSTFFQAIEAGVDQAGNVAVVFDLSPGNWPAAWTVIRNAATGAWSTPERVSTLGYKV
                     LLPTILSNPRTDTMYLVYLVADVGPVGVYAHKFDPATCTWGPAEFLPGSESAWYGVAG
                     NASRFPGVVDDDGNVTLFWGTPYTAYASRNVDGVWQPAVRLAPYTVTDVENFGGTAVN
                     AAGDVFGVFSRFDGLSRFCSFRYDAGAGWQPPEVLYAFANPYQTRVRAAFYQGRRAVG
                     VMLGEQGGLWQEVSFLFDGTSWQPGLIDIPDTYLSFFVDLVPQWGEVLQVCEVETIDW
                     VVQGIRATWLRAPNPGDLNCDGPVNVFDIDPFVMALTDPAAYAEAYPECYHLNADCNY
                     DREVNVFDIDAFVELLTGG"
     gene            27294..27605
                     /locus_tag="PLU99_00895"
     CDS             27294..27605
                     /locus_tag="PLU99_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15638.1"
                     /translation="MAVFDDGSGPALYVGGGFRLAGEVLSDDFAIWRCAPNYLPGDAN
                     CDGAVDTFDIDPFVLALVNPTAYRAEFPECLIENADVNHDGTVNVFDIDPFVALLTQA
                     G"
     gene            27934..28272
                     /locus_tag="PLU99_00900"
     CDS             27934..28272
                     /locus_tag="PLU99_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15639.1"
                     /translation="MNRTKNKPEKAKASDAARLQQLTADLDQEFVADKFGPLDAASRQ
                     KWQRAKRKRGRPVRGEGAQVISISIERGLLRACDELARRKKITRANLVARGLRAILAA
                     EGAAVLTDVR"
     gene            28539..30821
                     /gene="glgP"
                     /locus_tag="PLU99_00905"
     CDS             28539..30821
                     /gene="glgP"
                     /locus_tag="PLU99_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOO15640.1"
                     /translation="MPRTRSRACPRLELGHGTPHGSPHGAARGLKSQDRRGRQAGRVG
                     LFARLDALARNLWWTWNSTPQRLFAALDPVLWEATNHNPIATLARLSPDRRAALLNDA
                     DFASLLGRAEAELNEYLTARTWFQRTANRHDKTLRVAYFCAEYGLHEALPQYAGGLGV
                     LAGDHLKAASDLGVPLAAVGLLYRSGYYRQSFDSSGATRVAYPEYDFSLLPISDTGRQ
                     VAVPLDKRLVHARIWLVRVGRVPLYLLDTDVPENQPRDRAITRVLYGGDQVKRIQQEI
                     ILGVGGVLALRELGHDPNVFHINEGHAAFCTLQRLRELRQAGWPLEAAEERVWQSTAF
                     TTHTPVPAGHDRFPPEMVLRWLRPVIADIGLPHDAFLALGREHPESRREPFCMTVLAL
                     GLSQRTNGVSRIHGAVSRAMWQNVFEAARPEDVPIGHVTNGVHAPTWLAPEIEPLYQR
                     YLRPRWVGAGPEDDWWKHAARIPPAELWRVRTLLRRKLIAFIRQRLRQQIEAGFGSPK
                     ELAAAYESLDENALTIGFARRFATYKRATLIFRDVRRLAAILNDPRHPVQLVFAGKAH
                     PRDVAAQAFAQTVWRYARRPELRGRIVLLEEYDMNVARALIAGCDVWLNNPLRPQEAS
                     GTSGMKPALHGGLNCSVLDGWWAEAFNGRNGWAISPDKDVDGREADACAAALEQCSLS
                     RSHSRRTAAQLDRRDAETLYELLETQIVPQFYDRDRTGIPQQWVARMLESMRSICGQF
                     SAARMVGEYVRDYYLPALEG"
     gene            30861..31475
                     /locus_tag="PLU99_00910"
     CDS             30861..31475
                     /locus_tag="PLU99_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOO15641.1"
                     /translation="MLFDISPLVTESMAIWPGDVVPHREVHADFRHGERVSLSAWRSS
                     VHVGAHVDAPSHVKESGAAIDQLPLDTFVGPAQVIALHLPPQTAIEPEHLPARLAAPR
                     VLLATGSFNYTKPFDTRFIGLSAAAVDYLHERGVLLVGIDTPSVDLFTATDLPAHEAL
                     ARHEMLVLEGLDLSGVPEGLYELVALPLRLAGFEASPVRAVLRR"
     gene            31707..33521
                     /gene="dnaG"
                     /locus_tag="PLU99_00915"
     CDS             31707..33521
                     /gene="dnaG"
                     /locus_tag="PLU99_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOO15642.1"
                     /translation="MHNPLRDKVLEAVDLVEVVSEHVSLQRRGKEFIGLCPFHPDHSP
                     SLHVSPAKQIFKCFACGAGGDAIKFIQMREKLGYREALALLAQRAGISLQVSEADRAA
                     DASREQSRGVLSWARTHFQRNLAAARGAAAMDYALKRGLSRETIEKFGLGFAAAAWDD
                     LLMAARRAGVAAELLKQTGLVTANESGKVYDRFRNRLIFPICDNLGRPVAFGGRALDD
                     DPAKYLNSPETALFSKSRVLYGLDVARRAIAERREAVVVEGYMDAALLHQFGFGHVVA
                     TLGTAMTDSHLKLLRGVADRIYLCFDSDQAGMRAADRAVEVSLVGGVEVRVVMLEGAK
                     DPADLVVRSGGEAFRTALAKAVDALQFKWLQTVRTFGDGGLGSRRAAVESLIEFIASL
                     SPNAGPGPLEQGRLVRRLSELLGLPVEAVYEKLAAGRRRKVTGTADAASEDSPYAMAV
                     RGLPAGLVTASEEALGLLVAESRCVPLINEDVAYAFSLCRPWDRLYAIIRELSDGGAY
                     SQTNVIERCQEIDVCDLVGRCSRCTAGIENREEAFLRVRDRIRLELDLLRSEELDRKA
                     GAEKLSASDEVFDLARGQTRGRDFTFAACKCYAARPAS"
     gene            33647..35341
                     /gene="rpoD"
                     /locus_tag="PLU99_00920"
     CDS             33647..35341
                     /gene="rpoD"
                     /locus_tag="PLU99_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOO15643.1"
                     /translation="MIVGKPDIDPIEQSVNELIEASFKRKYLTWEELNETLPDEAISP
                     EKLESVLNRIEQAGIKLIDEIEAEKMRDADRKRVSVLEGGDSLDEVTEVDLTDAAAKR
                     IDDPVRMYLTQMGEIPLLAREEEIALAKKIELTRMAFRRKVLENDFCMQQAVDTMQAV
                     ADGRMPFDRTMKISTTESMAKSTIVKRLPANLATVRKLLELNQSDWQTVRDNRTPAAV
                     RKMARDRIAQRRRRAVTLLEELSLRTSRITPMMRKLNGMTCKMEALQHELKTRGKTLP
                     PEEVEAMKDEFNGLVDLVMEFPEDLRQRVNASQRVFGEYEEAKRRLAGGNLRLVVSIA
                     KKYRNRGLSFLDIIQEGNTGLMRAVDKYEYRRGYKFSTYATWWIRQAITRAIADHART
                     IRIPVHMIETMSKLRNINKRLLQKLGREPTIEEIAAEAKMPVGEARRVMKISRHPISL
                     DRPVGESEDSYFGDFIEDEKAESPVSTASNDMLRDRIEAVLKTLTYREREIIKLRYGI
                     GDGYTYTLEEVGKIFKVTRERVRQVEAKAIRKLQHPVRARKLQGFLDNARFGTAST"
BASE COUNT         6802 a        11529 c        11056 g         6213 t
ORIGIN      
        1 agttccggct cgtcggccgg cgccatcaaa accatgttct ggaaggctcg caggtatgcg
       61 atgtcgaggt agccgtggtg caccgcgccg tcgccgccca ccaatccggc gcgatccagg
      121 cagaagccga ccggcaaacc ctgcaaggcc acttcctgga aaatgtggtc gaaggcccgc
      181 tgcaggaacg tcgagtacac gcagaccagc gggcgcaagc cgctcttggc catgccggcc
      241 gcaatgtcca ccgtgcagcc ttcggcgatg ccgcagtcca ggacgttctc ggggtacacc
      301 tcgcgcacct tggccagccc cgtgccgtcg ggcatagcgg aggtcagggc gtgcacgcgc
      361 tggtcctcgc tgatcacctg cttgagcgct tcggcgaacg cggcggtcca gctcttgcgc
      421 tcactcttga ccacggtcac cgaatcgcca tcgacctcca gcttgccggg actatgaaaa
      481 gcgcaggggt ctttggaagc ccaggtacac ccgcggccct tgatcgtgtg tacgtgtagc
      541 agcaccgggc gcggcacatc cttcagcaac tgcaatatgt ccagcagatg cttgagatcg
      601 tgcccgtcca ccgggccgac gtacttgaag cccaattgct cgaatatttg gtgcggcgaa
      661 acggaggcct tgatgctgtc cttgaggtac tccagggcgt ctttcacgct ctggccgacc
      721 agcggcactt tcggcagcgc cgccgagact tgactcttga cctcctcata gaggctgctg
      781 acgcggaagc gggccaggta cttggccagc gcgccctgcg tcttggatat gcccatcgag
      841 ttgtcgttga gcactaccag gaattggcgc ctcagcgtgc ccgcctggtt caaaccctcg
      901 aacgccgtgc cgttgacgat actcgcgtca cctacaagcg ccaccgcccg gacgtcgcgc
      961 cccagcaggg catcggcccg cgccatgccg accgcggtcg cgatggccgt gccggcatgc
     1021 cccacgtcga acaagtcgta ctcgctctcc gccgcgcagg ggaagccgca tatcccgccg
     1081 gcctgccgca gggagtcgaa cgccgcattc cgcccggtca ccaacttgtg aacgtacgct
     1141 tgatgtccca cgtcccatag caagcggtcg cgggaaaagt cgaacacgcg gtgcagcgcc
     1201 agcgtcaatt ccaccacgcc caggttgctg gccaggtgcc cgccgcgctt gcagacgacc
     1261 tcgataatcc gtgagcggat ttcctccgca agctgcgaaa gctgctccag cgagagctgc
     1321 cgcaagtctt ccggagacct gatcgtgtcg agtattctgt cgcccaaaac gcttcctcgg
     1381 ttctcagtgt ttcttgccac cttagcgatc gcgtgcgatc acgtactcgg ccaactgcct
     1441 cagccggtcg gccgccgggc cgaacatctg caaggcgtcg cgggccgcca agtattcacg
     1501 ccgcgcggcc gcacggctgg cttcgaggcc atgcgccgcc ggataggtct gtttgccggc
     1561 tgcggcgtct ttgcccgccc gcttgcccag agtagcagtg gaaccggtgg cgtccagtaa
     1621 atcatcaatg atttgaaagg ccagtcccaa ccgccgtccg tagttgccca gggcttccag
     1681 agccgccggg ctcgctccag cacacagtgc cccgagccgg caggccgtct caatcagctt
     1741 cgccgtcttg tgcaggtgga tgtattccaa taactgcgca tcggtaggac gctgctctcc
     1801 ggccacgtcg gccgcctggc cttctatcat cgccagcgta ccaactgcca gcgtgcgctg
     1861 cagcgctacc gccagttcgg gcggatattc ggctgccaac agcgcgaacg gctgcaccgc
     1921 cagccagtct ccggcgagaa tcgcgcaggc ctcgccgtaa accttatgat tacagggctt
     1981 gccgcgccgc agctcatcgt cgtccatggc cggcaggtcg tcgtgtatca acgagaaggt
     2041 gtgaatacac tccaaggcca ccgcggccgc aacggcccgc tcctcgtcgc cgccacacac
     2101 gcggcaggat tcaagcgtca atacgggtcg gaaacgcttg cccggcagtg tcatcgaata
     2161 acgtacggct tcggccaggc ggctgtgcgg cccgactttc tccaaacaac tctccagacg
     2221 ggtgcacatg gcctgatcgg cccgttcccg caggctttcg taccagtctc gccaatctga
     2281 agaagactcg ccgcgcagca ttttttttcg gtcaaaaaat ggggcggcgt gcggaaaagg
     2341 gattccgaac cgtcgcgcca ttggcaaaac ttatactata cggcgtagcg gcctgactag
     2401 ctagcgcgct aaccacccta acagcgggca acacccgccg cagtttccgg agtttcccac
     2461 gccgttgctc gcacgatcga ggacgcccgt gtaggatgcc gcccgcgcgc ccctgagact
     2521 gagctgttcg gattaccggc tacatcagat tgtcgcggcg tcaccgcggc tgctatggag
     2581 ccggcgctcg gtgagcgccg gaggagcact gcatgaactt gaagcaagaa aggtactcca
     2641 agttcgccct ggttcgggaa atgatggaca ccgtggacgt tgtcgccagc ttcgatcccg
     2701 gccggacggc acaggtcgcc caggaaatca aacgcgtcgg ccgcctgctg ctggccggcg
     2761 agggttccag ccgcattgtg ccggccaaga atgccattcg aaaagccctg acctggggcc
     2821 tggatttgca ggtcgtcacc gacgggtcgc gccaagccgc ccactacgat ctcgccaact
     2881 tcgccgtctt ctgcgcatca aactccggcc gcaccaaaga ggtgatcctg ctggccagga
     2941 agctggccga ggcgggcaat ccgcaccgct acgggctgag cgccaatcgc gggacgctgc
     3001 tcgaagaggt ggttaagcac atttttgtgc tgacgtgtgg ctgggagcag gcggtggcgg
     3061 cgaccaagag cgtagtcgag caggcgctgt tttatcagtc gttactgtgc cacgtggccg
     3121 gcggaaaaat gacccctgac ctggacggcc tggcggagaa gattcggcac gtcctgacgc
     3181 tgcctatcga cccgcagatc gtacagaagg cgatgaaggc gacaacgatt tactttgcgg
     3241 gctacaacga cggggtggcc gaagagctga cgctcaagac aaacgagatc acgcgccgca
     3301 agtcggactt cctcgagggg acatacgcca tacacggcgt cgaggaggtc atggacccga
     3361 gcgacgttgt gttcgtaatc gacccgatcc aggaagaagt ggagaagttc caggaagtgc
     3421 tggtaaaggg cgtgggcctg acggtgatcg cgatcgccga ccgcccgacg ccgtttccca
     3481 cgatccaggt gccggcggcc ggcgagctga cgcccatggt ctacctgtgt accggatgga
     3541 atctgctggt tgagatcggt ttggccctgg gcataaacct ggataagccg gagcgtgcgc
     3601 gaaaggtcgg caacgaattg gtggtataac cagcccgcga ctcgagcttc cggcgcgtgg
     3661 tgtagtgcga ggcttggccc tgccaagctt gggttccatg cccaaggttg ggtgccatgc
     3721 ccaaggttgg gtgccatgcc caagctctaa gcgcgggcat gccttcggcg tgtgcctggg
     3781 tgccatgtcc aagctctaag cgcgggcatg ccctcggcgt gtgcgaaccg aaagtttgca
     3841 ccccccgcac ggttcatcgt attggttcat gaggcgcaaa atcgggcaac gcgctaaata
     3901 gcagcaaggt gttcggctca cgaggataag agcatgcgtg aagtcaaagt actgccgctg
     3961 gaggagttcg ggcgcgagtt tgttccgccg cagtaccggc ccgcaggcca ggacgagtac
     4021 tacttacgaa accagcaagt cgggcgcccc aaggactatt ggcggcacct gcgggccgac
     4081 gaaatcgaga cgctggtcaa gaacaacaac acctgcgctg actgggacca catgctcgtg
     4141 gccgacccct tcgatccgcg cctgatcaag aacagcgagt tttacggcct ggtgcggatc
     4201 gggcggatgg agcgcgtgat tctggaacac catgatttgc agaagcccgt gggcatttcc
     4261 ggcagcttga tcatcaattg cgacatcggc gataactgcg ccatcaacaa cgtccgccac
     4321 ttggcgcatt tcatcctggg cgcgaatgtg atgctgctga acattgacga aatgcatacc
     4381 accaactacg ccaagttcgg caacggcatc gtcaaggacg gcgaagacga aaaagtgcgc
     4441 gtctggctgg acctggtgaa cgaggcgtcc gggcgggcgg tgatcccctt tgacgggatg
     4501 acgaccgccg acgcctatct ctgggcccgc taccgtgaag atgccgagct tcaggcccgc
     4561 ctgcggcaaa taacggaaaa ccagttcgat tcgcgccgcg gcttctatgg caccgtgggc
     4621 gacgcctgtg tcatcaagaa ctgccgcatc atcaaggacg tcaagatcgg accgtgctgt
     4681 tacatcaaag gcgccaacaa gcttaagaac ctgaccatca actccagcgc cgaagagccc
     4741 acgcagatcg gcgaaggcgt ggaactggtc aacggcatca tcggcctggg ctgccacgtc
     4801 ttctatggtt gcaaggcggt acgtttcgtg ctgtgcgaca actccaacct caagtacggc
     4861 gcccgcctga ttcactctgt attgggcagc aactctactg tttcctgctg cgaaatcctg
     4921 cacaatctgg tgttcccggc acacgaacaa caccacaaca actcgtttct gacggccgcg
     4981 ctggtcaaag gccagagtaa cgtggcggcc ggggccacgc tgggctcaaa ccacaacagc
     5041 cgcgccaacg acggcgagat tgaggcgggg cgcggctttt ggccggggct gtgcgtgacg
     5101 ctgaagcact ccagccgctt tgccagcttc accttgctga gcaagggcaa ttaccaggcc
     5161 gagttgaaca tcccgctgcc atttgcactg gtgaacgaca atcaggccgc gcagcgtctg
     5221 gaggtgatgc cggcgttctg gtggcgctac aacatgtatg ctctggcccg gactacttgg
     5281 aaagtacggg ttcgcgacac gcgcaaaacc aagacccaga acattgagtt tgattcgctg
     5341 gcgccggata ccatcgagga aatcttcgac gccctgcgcc tgctggaaat ctggaccgcg
     5401 cgggcgcgac tgcgggccga tggaaaggac gcggccgaac acagccttga agatctggcg
     5461 gcgctcgggc gggaactgct gctaagcagc ccgcagcgca cggcaggtct ggaagtgctc
     5521 ggcgagaaca tggagaagag cgaccgcaag gtggtcatta tgaagccgcg cgagggctat
     5581 ctcgcctatc gcgaaatgct tcactactac gcggtcaaaa acctggtggc ctacttgtcc
     5641 gcccatccgc aggccacctt ggacaccatg ggcaaggacc tggccggacc gcggcagcgc
     5701 gagtgggtca acctgggcgg acaactcatg cgcggcccgg acagcgcccg gctcattagc
     5761 gagatcaagg ccggcaaata ccgtaactgg tttgagattc acgcggcata cgacaagctg
     5821 tgggcggagt atccaaagca caaacagcaa cacgcgctgg cgacgctact cgagctgctt
     5881 ggggtgacca aattgacccc ggagatctgg gacgcggcgc taaccgaggc ggtgcgaatc
     5941 caggaattcg tctgccagca ggtctacatc agccgcaaga aggattacga caatcccttc
     6001 cgccaagcta cgttccgcaa tgcggaggag atgcgggcgg tcctcggcac gccggaggag
     6061 aacagcttcg tccggcagac gaagaaggag acggaggctt tcaaggcgct ggcggaggag
     6121 atgcgcaagc gagtctagaa caaaagcact cagccgcgcc gctgatcaaa catgcagttc
     6181 agcgctgggc gccggcccga ggtccagccc cttcagcgcc ggaccaagaa tgcgcttcaa
     6241 gtactcgcgc gtctgttgcg gggccaggcc gacgtagcgc tgtgggttca gcaggccgga
     6301 ccatttcacg tggacaaacg ccggatcggc cttcagacgc tccagcaggt cgttcggacg
     6361 gccaagttgt ttcacgccct cgccggcggc ctgactgtgt tcgcgcaaac gctcgtgtag
     6421 ttgctggcga tcgccgccgc ctgcgacggc ggccatcaga atctcttcgc tggcgatgaa
     6481 cggcagttcg gccgccaggt gtgcctcgat catggccggg tagacgacca ggccgtcaaa
     6541 aatgttgttc aacaggatgg ccagcgcgtc ggcggtcaag aaggcctcgg ggacgacgag
     6601 gcgcttgttg gcggaatcgt ccagcgtgcg ttcgagcatc tgcgccgcat gcgtgtgata
     6661 ggccgagctg atcaggctga ttaggtagcg ggccaggctg gtggcgcgct cgcagcgcat
     6721 cgggttgcgc ttgtacggca tggcggaact gccgacctgc cggcgctcga agggttcttc
     6781 aatttccttc agcatggcta gaatgcggat gtcgttgcac agcttgtgcg tcgcgccggc
     6841 cagcagcccg agggcggcca gaatctcggc gtcgatctgg cgcgagtacg tttgtcctac
     6901 gaccggatag cagtgctcga accccaggcg gcgaacgaaa gactgctcca gccggcgaac
     6961 cttcgcccgg tcgcccagga gctggaggaa ggaagcctgc gtgccggtcg caccgcgtaa
     7021 gccgcggcag gcgaggttgc ggcggacgcg atcgatggcc tgcgtcgcgc gcagcagatc
     7081 gtcgagccac agggtggcgc gcttgcccac ggtcgtgagc tgggccggct gaaggtgcgt
     7141 gaagcccagc gtcggcaaat cggcgttact ctcgcaaaat ggggccaaca cgcgcattgc
     7201 cgccaccagc cgccgctgga gcaaatcgag cgcggcgcgc atcaggagca ggtccgcatt
     7261 gtcgaccacg tccatgctcg tgagccccag atggatgatt ccgcgggcgg cgggggcggc
     7321 gtccccgaaa gcgtgaatgt gggccattac atcgtgccgc gtttgacgct cgtactccgc
     7381 gacggcgtca aaatcgatgt catccaccgc ggcctgcatg gcgcgaatct ggccggcggt
     7441 aatgggcagc cctaactcgc gttccgcttc ggccgcggcc acccagatgc ggcgccaggt
     7501 ggagtagcgc tgctgcggac tccagatcgc gaccatctcg cgcgaggcgt agcgcgccgc
     7561 gagcgggtgc tcaaatgtcg gcttcgattt gaaaatcgaa cttcgtggct tcgtaaccgg
     7621 cttgcgctgg cgcttcgggc tcgggtcagg cggactggga cggcgcggtc tgctcgggcg
     7681 cggcatgtca cgactccagc gcctgcttta catcggcgat caggtcttcg gttgcctcca
     7741 ggccggtgtg caggcggatg atccaggcgg gctgctgcgg gtccttcgaa ataaacgtcc
     7801 caccgattac caggctctcg tagccgcccc agctcacgcc gatcgtgaac aggcgagtgc
     7861 ggtccatgaa gcggttcgtc gcctcgcgcg actggtcgtg cagctcaaaa ctgaacagcc
     7921 cgctgtagcc gcgaagctgg cggcggccga tcgcgtactg cggatggctc tccaggccgg
     7981 ggtgatacac gcggcggacg gccgggtgct gctcgagaaa gcgggccagc gccagcccgc
     8041 tgcgctggtg ctgttccatg cgcaccttca gcgtgcgcag cccgcgaatg agcagccagg
     8101 cggcgaatgg gtcgcacacg ctgccgagca gctccagctc ccggaaaacc cggcggcgaa
     8161 gggtgtcttc acgcccggcg accaggcccg cgactacgtc gctgtggcca ccgatgaact
     8221 tcgtggcgct gtgtacgact agatcacagc ccaggtccag cggggtctgg aagcacggcg
     8281 tcgcccacga gttgtcgaaa accgtgacca ctccacgcgc gcgtgccgcg gccgcgatcg
     8341 gcgcaacgtc cggcatctcg aacagcccac tggtcgggct ttcgaggtac atgagcctgg
     8401 tgttcggccg gatggccgcc tcgaagtcgg ccgggtcgca gctatcaacg aaagtggtct
     8461 cgacgccgaa ccgcggcagg tagtcgcgca agtagcgtcg ggtcggccaa taaatcgtgc
     8521 cgctggcaac tacatgggcg ccggcgtgaa cgcaggcatt aatggcggca gcgatcgcgc
     8581 ccatgcccga acccagggcc agggcccagt tgccgtgctc gaggcgggcg attttcgctt
     8641 ccagcaccgc ggtggtggga ttgccgacgc gggtgtactc gtaatacgga ctatcggggg
     8701 cttcgcgctg ctcccaggcg gctgagtcgg ggaacatgaa cgtcgaagcc tggtatatcg
     8761 gcacggcgac cgcgccccgc tgggccatgc ggtcttcgcc aaaatgggca caaagtgttt
     8821 caaagtcggc gccgggcacc ggatcatgcc gcggcggcgg cggcgacatt tgaaaactat
     8881 aggggtcgag agctagggga tccatgtcca caaaatcgcc gcaggcgcgc cggcggtcaa
     8941 ggggcgaccc gcgtttggca gccgccattc atcaatccgg cgtgatgccc ccgcacggcg
     9001 ccctatggct tgccgttcgg ggagcgcagc ggagaaaggt ggaaaacggc cccgcagccg
     9061 gggcggttgc agcgcaccgt cagcgtcggg atggtcgcga attcctgcgg ctcggtattg
     9121 agcatcgcca gaacggtcgg cagaatcgaa tgggcggcgt ggatgcacaa cccggcgggc
     9181 agcgtggccg ttatgctgaa ttccggcggc agcgaggcgg cggccgggca gatatcgatg
     9241 tttgaagcgc gggccagcac cacgcgtacg cggtgcgcct cgaccgctga ggccggcaca
     9301 tttggctcgg cagcggatcg cgcgcccgga tcggcccagg acggcaactt tgccggatca
     9361 ccttcggctc gcgctcctgc cgcctctccg gcgccgggat tttgagacga gcctgcggca
     9421 gccgtggaat ctgcatcgaa tacgatcacg tcgctctcgc ggtcgagcgc catattcaca
     9481 agctcgtcgg cgcgtgcgtt ctcttcgcgt ttgatgtgcc gcagcgtcca gcgcggggcc
     9541 cgcagcagga aaagttgggc ttgctcgtag agctgctgaa gttttgggct cttaacgtcg
     9601 tattcgcccg taacctgccg cacgagcaac tcgctgtcgg agtaaatcga aagcgtctgc
     9661 gggccgcact tttcggcgcg catcagcgcc cgcaacaatg cctgatactc ggcggcgttg
     9721 ttcgtctggc ggccgaggaa gtagcccgcc tcgtgaatcg gttgaccgtc gtcggtgtgg
     9781 atcacaactc cggccgcagc cgggccggga ttgccgcgtg ccccgccatc aacgtgaata
     9841 atcaggccca cgcgccgctc acggaatcac ttcctcttat ctatatatag gatacggccg
     9901 caattcgggc agctctgcac atcgtcgcgg accatggcgg cgttgaaatg ttccgcggag
     9961 atgctcatgt ggcagccgct gcagatgaat tcgtcgcggc gcgggtgcgt gcgttcgatt
    10021 ttcgcgagga cttcgccttc gtaccgttcc gacaggcgtt cgaagaggtt gacggttctt
    10081 ccttcgagct gggcgatgga ttcctcgcgc cgcgcctcca actctttcag cttgtcggaa
    10141 aaggtcgcca tggctccgct ggcctgggct tgaaaatcgc gcaggcggtc ggcctcggcc
    10201 cgttcgacct cctctgccgc ggccacagcc tggcgctgtt tgtccatttc ggccatgagt
    10261 tccagttcgc ggctctcaag gcggttagcg tctgcttttt cgttattgag gtcagccagg
    10321 acggccgcgt attccttgtt ggtccggacc gtgttgaggt gctcgcgcaa gcgggatacg
    10381 tgcgcctggc ggcctttcag atccagatca atcgtatcga tttctatctg cgtgcgtcgc
    10441 aggcgttcct gttctgcggc cagccggtca cgcgcggctt gcaacttggc ggcttggcgt
    10501 tcgaccaggc gctgtttctg cgcaagctgg cggcgaatat ccacgagctg aagttcgata
    10561 tcctgaagct cgagcaaggc gccgagcgtc gttcccattc aactctccca cgggtgtcaa
    10621 gaacccatga ttatgccaca gatcgacgcg ggcggcaata gaagccggaa catcagcagc
    10681 acccaagccg gccaaccgtg gcaaaaaggg ggcagggggc aaccggtagc gtgcctgggc
    10741 gtcgggccag ggcgtcaacg tgtgcggcgt gacgtcgggt gccatgtccc gtcgggtgcc
    10801 atgtccaagc tgtaagcgcg gacatgccct cggttaagtg catctcaagt tccagacggc
    10861 caaccgccgg cccgtccggc ctcctggcac cgcgtcccca ccgcccattt tttgacggcg
    10921 gcagcttgca cgggccgtcc gctggttgag aatgccgaaa aacagtcagg agtaggcatg
    10981 acacgcctgt tcgaaaaata tgcggggcgc gctacgcgct gcatgttaac gggacgctgg
    11041 tgcggagcgt ttctggccgt cggcctcctg agcgcctggg ccgccattgc ctgggccgcg
    11101 ccgccggcgg agcatcgcca atccggcgac ctgcggcgct cgccggtagt cgacgttttc
    11161 gagcgctgcc gcgaggcggt cgtgaacatc agcaccacgc gcgtggttca gatgcgctcg
    11221 gtgagtttgt ttgagagcat atttgacttc gccccgcgcg tgcggcggca taaggttcac
    11281 agcatcggct cgggagtggt catccattca gcgggttacg tggtgaccaa cgcgcacgtc
    11341 gtggcccagg cttctgacgt gcaggtcacc ttctctgacg gcaagagtga aaccgcggaa
    11401 attgtggcgg ttgaccccga gcacgacctg gccgtcctga aggtttccgg cgagcacgcg
    11461 ttcgccagcg tgaaactggg gcacaccggc gacatcctgg tgggcgagac cgtggtcgcg
    11521 atcggcaacc cgctggggct gcagcacacg gtgaccgcgg gcatcgtcag cgcgctgggg
    11581 cgcgacctgg tgttcaacga caatgtgacc tacaaaaacc tcatccagac ggacgcgtcg
    11641 atcaacccgg gcaactcggg cgggccgctc ctgaacgtaa actgcgaatt gatcggaatt
    11701 aacaccgcta ttcgcggcga cgcgcagaac atcggtttcg ccattccggt cgaccgcctc
    11761 tgggagacgc tgcccgtcat gcttgacctg gagcgccgcg agcgcgttca agtggggctg
    11821 cgcgtcagcg gcccgcgggc ggaggttgag gaggtgcgcc cgggcagtcc cgccgccgac
    11881 gccggcctta aagtccggga ccaaattctg cgggtaaacg gcgagccggt tcgggacggc
    11941 atcgactact acgtgcacct gctgaacgcg caccccggcg acaatatgaa cctggccgtc
    12001 aagcgcggcg gaagaaccct ggatttcgac gtacccatac gggtcacgcc gctgcccgac
    12061 ggcgacagcc tggcccgcaa gcttttgggt attaagctga caaccatccc cagccgcgtg
    12121 cgccgccaat atgacctggc cgggctgacc ggcatgatgg tggaggaggt cgagcgcaac
    12181 ggcccggccc gcaaagcgct ggagcccggc gacatcattc gccagatcga gaacatgccg
    12241 accaccagcc tcaagcaggt cggcctggtg ctggagcagg tggttcccgg cgacgcggtg
    12301 gacatcgaac tgatccgctt cgagcgcgac ggcgtctacc tgatcaacat ctccatcccg
    12361 acgcggaagt agaccaacgc ggaagtagaa cattcgccgc cagaatgcgc ggatggcgcg
    12421 tgtgtgcgcg cccggcggcc atcagctttg agctatcagg tgtgacaaga agttgccgcg
    12481 ggtgccggag cacgtccagc gcgaggtcaa cgtcacgtgc gttgtaagag gccagattga
    12541 ggacactgat acaaggcaat tgcaataaag ttataggaca tacaaagaag taatacgttc
    12601 tctaaactgc gaccctgtca gtacttatgt gctacgtcag cgaggcgcga ttgaattata
    12661 acagaaaaaa tgttatattt cattgcttgg tttgacgatg cttccgcgga ggcgcgcagt
    12721 ggaagagctt ggactttatg aggtcaggtt tattggaccc gcgttttttg aagctcgaaa
    12781 cctgtgtcgc gagaccttcg acagaatggc tcttcgggac cacctgagaa agctcgagca
    12841 ctgggagcac gacgatgtgg gtggtttggc ggtcgacctt gactggtttt ggatacgtga
    12901 tttcaaggaa ttgcgcttag cggaggtgct catcgacgat gaactcggcg ggcatgccga
    12961 tttgcgagcg gtcgttttcg tgcctacgga ggcgtacccc tatgcccgcg acgggaagcg
    13021 cgtgatatgg gtgctgagcg tgctggacaa acaggacgat cagtacacgc tcgccgaaac
    13081 cgccaacctg tggctgcggc gaaaatcagt gcagaggtat tacgaacgag aatctgcggg
    13141 tgagtacgag cgtttgaatg atttaaccga ttgagccgcg attgtgcacc cggggagtta
    13201 taaaatgaaa gttgatagga agccgatgct ctcacaagaa atacccaagg agcaactcgc
    13261 cgtcggggcc gcattcctgc gcgcgttcat ggaatgcgat gatgaaattc agaagcatgt
    13321 cgtccaactg gccaaattag ctggttcgcc ggaagcgacg gatagagaga agacgatggc
    13381 tctctattcg attgccgaag cgctgcgacc tgtaatgcat aacggtcacc tgggcatctc
    13441 ggtcgacgag gctgaccaga ttgcggtcga ggaccggccg gacggtgcgg cggcgctggc
    13501 gcgcttgcag aatcaaaaga aagagttttc cgagcgcctg gcagcgctga tggagcggaa
    13561 gaacatcagc caggctgaac tggccaaggc gatcggcgtc agccagccgg ccatttcgct
    13621 gctgcttgcg cgcaagtgcc gcccgcaaag acgcacggtt gagaagttgg ccgaagctct
    13681 gaacgtcgaa actcgggaac tctggctcga gggatagtca aacagaagtg aacgcatttg
    13741 caggcccggc tgccgttggg tcgccgggcc tttttcgtag cacagcgtgc tgacgcaagg
    13801 gcaggggctc ggagatcaac ggagcatcga gagcgcgact acttaacggc cagcaagggc
    13861 cgttaacgcg gcaaactcga agcttgcgct tcaggtagtg ccgcaggccg ggcttcccgc
    13921 ccaggcggcg cgcagttcgc tgaccgggac gctgtggccg cccacgctca gtaggtcgtc
    13981 gcgcctgacc attcccaaat ccagaatccg cactccgcgc cgcgacatca ttgcgcccag
    14041 cttccccgcg tccgccgttt cgatcacata ccccgcgcac attcctgcaa agggcgacgt
    14101 gttcgggttg ccctcaccgg cgcttcgggt tgtttggccc tggcttgcgc ttcgggctcg
    14161 gattgcatgg ccctcgctgg caccttgcgc tcgcattgga ctgagcgcct catcggcaat
    14221 cccaaccccg cgcccgcccg cgaacgccat ctcggccacc gccgtcagcc atccaccatc
    14281 gctgcagtcg tggcacgccg cgaccagccc ctccgcaatc gcatctgcga cagccacatg
    14341 aatgccggcc gcctcagccg gatcgaatcc gacctgcgga actccaccga ccaggaacag
    14401 ctcgttgccg gacgccttca gatcgctcgt gacacagcgc gtcacgtccg aaacgatgct
    14461 gatggcgctg atcagcagcg tgcccggaat ggccagacgc cccgtctgcc gaatgcgatg
    14521 ctccaactca ggcaaaatgc ccctgagccg caccgcgtcg cgatcatcgc cttccgacat
    14581 cccgcgcaac agcgccaaca aaggctccac gtccgccgca tccagcgcaa actcgttgtt
    14641 cagcgaatcc ttgcccgaaa tgaacggcac accgtacgcg accgccacgt catagcacgc
    14701 ctggcacgcc cgcaccaggc cgccaagctg ctgcggatca tccgcccgcc cccagcagaa
    14761 attgtccagc accgccgtct gccgcggatc gcctcccacc gccaccacgt tccgcaccgc
    14821 ctcgtctatg ccggccgcgg ccatccagta tggatcaacg tcgctcagct ccaacgccaa
    14881 cccgcaaccc agcgcgattc cccgccgcga acccgccacc ggcctcagaa ccgccgcatc
    14941 gcccggcccc tgccccggcc ccaccagcgg cttaatgact gacccgccct gaacctcgtg
    15001 atcgtattgc cgaatcaccc attccttggc cgcgacgtcc ggccgcgcca gctcgcgcag
    15061 cagcatccgg cccagatcat cgcaggcgcc agccgagttt tcaccgcgct ccggcgcggg
    15121 cccggattgg gagggctcgg attgcgactg tggcgggtgc cattccacaa ccgcgctgcg
    15181 tttcggcacg ccgcggtgca gaaactccat ttccaggtcg ccgacgatct ttccttcgta
    15241 gtggacgacc agccgcggtg ccccgccgta gccttccgag ccgaaaacgc cgatgacgct
    15301 cgcctccacc tcctcctcgc gcatgatttc gagaaaacgc tccaggtgct ccggcggcac
    15361 cgccagaacc atgcgttcct gcgcttcgct gatccagatt tcgtcatagc gcagtcccgc
    15421 gtacttcagc ggcaccttgt ccaggtgaac cgtcgccccg acgtgctcgg ccatctcgcc
    15481 aacagcgctg ctcagcccgc ccgcgccgca atccgtaatc gcgctgtaca agcagccgcg
    15541 cgcgtcatcg cgcgctcgca gaaccgcgtc cagaaagcgc ttctccgtga tcgcgtttcc
    15601 gatttgcacc gcgtgcgaga attcgtccgc gtgcgtgtcc gtcagctcgg ccgacgagaa
    15661 cgtcgcacca tgaatcccgt cgcgtcccgt ccgcccgcca atcagcacga tgtgatcgcc
    15721 gggtcgcgcg gctttctcga ttttgtcgcg cggaatcaac cccacgcagc cgcagtacac
    15781 cagcgggttc ccaaggtaac gcggatcgaa gttgatcgcc ccattgaccg tcgggatgcc
    15841 catgcggttg ccgtagtcgg ccacgccttt gacgatccca cgcaaaatcc gccgcggatg
    15901 cagaacgcct ttcggcagct tgtcggccgg ccagttggaa ggcgcgacac agaatacatc
    15961 agtattagcg atcgggcggg cgccgagccc gcagccgatt acatctctaa tcactccgcc
    16021 gattccggtg gccgccccac cgtagggttc gatcgccgac gggtgattgt gcgtctcgac
    16081 tttgaacgcc acgccatgct gctcgtcgaa tccaatgatc ccggcgttgt ccttgaagac
    16141 ggacagacac tccggaccgc gaccggcttc catcaattct gccgtcgcgc gcccgatcgt
    16201 gtccttcaat aggttttcgt agcggcgttc gaaggcgacg ccggcgcgag acgccccggc
    16261 tccgccaggc agaggctcgc cgcgatacac gatctcgctc ttgagcgtct tgtgaacgca
    16321 gtgctccgac cacgtctgcg ccagcgtttc caactccagg tcggtcggat cccgccccag
    16381 cggccggtag tgagcctgaa tcgccttcat ctcctcgcgc gagaggaaca aatgtccgct
    16441 gcgcgacagc tcattcaagc ccgcgtcgtc caactcgcgg atcggcacgc gccgcagaac
    16501 gaattcgaag accggcggtt gcggcgccgg cttcaccccc gccgttccga taaccacctc
    16561 ctcgatgcag tcgttcgcca gcacacgccc cgccgccgac cgcagctcgg cctccgtcat
    16621 ctccccgaaa agaacgtacc gccacgctgt ccggacggcc tgcaccccca ctccctccgc
    16681 gcgcagctcc gccaacgtgc tctcggcgac tggatccatc accccaggcc gccgatgaac
    16741 ctcgaccgtc cgggccttgg ccggcggcac ggccgactct ccctcgcgac gaaccgagaa
    16801 ttcttcggtc accgggtcta tcagcagctc gacgacccgc tcgatatccc gcgctcccag
    16861 ctcgccgtcc aaaaagaaca gccgctccgc gtagccgctc tccacggcca acccggcctc
    16921 acgtagtctg aagatcaggc ttcgatcgga cgcggggcga ccatcaaccg gtcgcggtcg
    16981 gacttcgatg caatagacag tcataaggat ggagattgtc gcacagccgc cggagttagt
    17041 ctatccagtc cggacgctgg aacttctcgg ccagccattc gttgcggatc tggacggccc
    17101 gcgccagtaa acgatggacg ttgccgtcct cgtggcgctc cagccaggtg tccaactgtt
    17161 tcaggtcgtc gacgagctgg ctgatggcca cgcggacctg ttcgcggttg gaggtgagga
    17221 tgtcctgcca caaatcggga tcccgcgacg cgatatgggt catctggatc agccccggcg
    17281 cggccacgtg cagcccctcc tgccgcgcgg ccaggagcag cgtcagggcc gaaaccacgt
    17341 gcggcaaatg gcttacgcga gctatggttt cgtcatgcgc cgcgggcgac atttcggtca
    17401 cgtgcgcccc ggttagctcc cacattgagc gaacgcactg cacggcttca ggcgcagtcg
    17461 ccgaaacggg cgtcagtatc acggttttgc cgtgaaacag gtccgcgcgg gcgaaagaca
    17521 cgccgcgctg gttgaacccg gtcatcggat gggccccgac gaaacgacaa gggtggctca
    17581 gcgtggagcc ggcggcgttt acgactggga ccttcacgct gccaacgtca gtaacgatgg
    17641 cggcttcgtc caggatcggg gcaagctgcc gcagcatacc cggcatgacc gaaagcggcg
    17701 tcgccaggca gaacagccca acgtctttgg gcagatccgt cggccgggtc gcgccggagt
    17761 gcaccgcgcc ggtctcgatc gcggcctcca gatcgcttgg atcgcgagcc aaggcaagaa
    17821 gcttcccctc gaacccggcc gcctgtagcg ccaagccgag cgatccgccc atgagccccg
    17881 ggccgacgat gcaaacgcgt tctatctctt tgatttccag gagcttacga cctattcagg
    17941 acctgaggca aaccatcaaa tctttagcaa cgcctaaaaa tacgcttgca tttggcgctc
    18001 catagcaggt agtattcccg tccaacagga cgcagaagat gcgttcgaaa aggcgcggtt
    18061 agcaatcagg tagatatgag cacgcagccg agtaccaaga acgggcccca gccttaccta
    18121 ctcgatttcg aacgacccat aaacgtcatc gaacaggaac tgctgcgcct cgaggccgcc
    18181 gcagccgacg gggggagtga tttctcagcc attgagaaaa aacgcgcaga actcaacgaa
    18241 gcacttcgcc aggtctatgg caagctgaca gcctgggatc acgtgctggt ttcgcgccat
    18301 ccgcgccgcc cgctgcttcg cgattacctg cgcctgatca ccgaagactt ctacgagctg
    18361 cacggcgaca aggtctacgg cgatgaccgg gccatggttt gcgggtttgc ccgaatcgcc
    18421 cggcagcgcg tgatgattat cggccatcag aaaggccgcg acacacgcga gaagatcgag
    18481 gccaatttcg gctgtgccca cccggaggga taccggaagg ctctcaagaa aatgcagttg
    18541 gcggcgaagt tcaaggtgcc gatcgtctgc ttgatcgaca cgccgggcgc ctatccgggc
    18601 attggtgctg aggaacgggg catcgcgcac gcgatcgccg taaacctgat ggagctatca
    18661 cgcctgccgg tcccgatggt tgccgccgta attggcgagg gcggcagcgg cggcgctttg
    18721 ggcatcgccg tagccgacca ggtggccgtg atggagtacg cgtactactc cgtcatcagt
    18781 cccgagggct gtgccgcaat cctatggaag accggcgagc aggcgcctcg ggcggccgaa
    18841 gccttgcggc ttactcccgg ccacctgaag gaattgggct tgatcgacgc ggtcatcccc
    18901 gaaccgctcg gagcggccca ccgcgacccg gtcggtgcgg cccgcaacct ggaggcctgg
    18961 atcatccagg ccctgcgcga agtacgccgc ccccgtatcg aaaccctcct ccgcagacgc
    19021 tacgagcgac tgcgaaagct gggttcagct tttacgacca aaagcggtat ggaggccaag
    19081 actgtgcggt cgcgcggccg gcgcctgggt gcgtctcgcc acgtggcggc tctggactcg
    19141 ccgtcttaac cgggccgaac tgggacggcg aaccgaattt aggctcgctc cagatcaaaa
    19201 tacctgcgac acgcggcagg tttttgtata taatttggtc aaaaggcctt cccaggtggg
    19261 cgggaagtcg taaagaccat taggcggagg tagccgagtt gcccaggttt ttgcatctgg
    19321 gtgacttggg tgttggtaga tagatagttt gagaaacgtt tcgacgctgt gcggcggttc
    19381 gagccggatg tcgcttgggt gcatgctggt cgccaatgac cgaacccgcg gcaaacggct
    19441 cggataagac cggccgaatt caacggccac ggggcgccgc gaaaagaggt tgtgttttac
    19501 tagcgaatct tgctacaatc gactttttgg cttgcttgtg cggaaataag gatggcatct
    19561 gaagagacta tgaatcgttc gggaacgtgc gaggcgctgg ttcaagccgc cgatctgcac
    19621 gtgtttttgt acgatcgacc cctgcacccg gagctgtttc gccattatcg cgattttcgg
    19681 gtggcccagg gccgctatta tgcggacgtt tggatcgttg ggttgaacca cgtcgtcacc
    19741 gtcacctccg gtcagcgctc catgaccgag ctgctcgcct gcgagagcga cgtgctgccg
    19801 gcccgcggcc tggtggcgcg cttccgcctc aagggcgaac gcgaccagga aaagcatctg
    19861 ccggacggct ggctgcacat gatcagcaca caggttgaaa ccatggacga gccgctttac
    19921 aagtctgtac ataacgactt gttgcggcac gcctgcaagc gcggctggtt ccatgcttac
    19981 gaggagtggg ccgacggcga cctggtcccg ttctcgtaca tcgaccacga ggagcgcgac
    20041 gcggagttcc acattcacgc gttccacgct ttcccgcacg agcgcacgct gattaagaca
    20101 cagtcgattt tcgagatgcc gccggcctaa gccgttacgg cgtattctca gcgaccatgc
    20161 ccggaaggcc ggccccgtgc gcaccgccgg agccagacgc gcggaaaaga gccgcgccgc
    20221 ctgccgggga tggtcgcgct gtttagcccc agcgcgccgc ccgcgccagt gtccgccgca
    20281 cgtggcgagg tgggcgaccc gcgaagtaga cggaatcgat gaaggcgcga gcgacaatgc
    20341 cgagcgagag ggacatggtt cacgtaatcg gggcgcgcga gcataacttg cgctccgtca
    20401 gcctcgacct gcccaagaac cggctcatct gtttcaccgg cgtgtctggc tcgggtaaaa
    20461 gcagtctcgc gttcgacacg ctctacgccg agggacagcg ccgctacgtc gaatccctca
    20521 gctcctacgc ccgccagttc atgggcgaac tgcccaagcc ggacgtcgac cagattaccg
    20581 gcctggcgcc gtcgatctcc atccagcaga agaccagcgg ctggaacccg cgcagcaccg
    20641 tcggcaccat cacccaggtt cacgattaca ttcgcgtcct catggcccgc gccggcaccg
    20701 cccattgcac caactgcggg cggccgatca ctgcccaaac ccgcgagcaa ctgctggcac
    20761 gcatcctgga gctgcccgag ggcacgcgct tcatgctgct ggccccgaaa gtccgcgctc
    20821 aaaaaggcga gcacaaggac ctgttcgccg agctgattca gcaaggcttc gtgcgcgccc
    20881 gcgtcgacgg cgaggtgatc gacctctcga atccgccgcc gctcgatcgc taccgccgcc
    20941 acgatatcga ggtcgtcgtc gatcgcctgg taatgcggcc caacctccgc ccgcgcctgg
    21001 ccgaagcgct ggataccgca ctcgaagccg gaggcggcat cgcgattctc gccaccctgc
    21061 acgaagacga gggtcagccc gaaggcgtag ggcagccggc gccggccgga cgcgcccggc
    21121 tggcgcacat gcgctcccgc aagcagaccg ccaatgacgt cctgctctcc tccgcttacg
    21181 cctgcacgca ctgcaacctc ggcttcgagc cgccgaaccc gcaaatgttc agcttcaaca
    21241 gcccgcaggg catgtgcccc cattgcgacg gcctcggcac gcgcttcgat ttcgatcccg
    21301 agctgctcat ccccgacccc tcgctcagct ttctcgcccc gtgcgtggcc gccttccgcc
    21361 acgagcccgg ccgctggcgc cgccatattt atgagggcgt cgcccggcac ctgggcttcg
    21421 acctgcgcac gccctggcgc gatctgccgc ccgccgcccg aaacgcacta ctcttcggca
    21481 ccggcaatac gcacatcacg tacgaatggc gcggccgcta cggaacgtgg aagcacggcg
    21541 gcccgttcga aggcgtactg gcggagctgc acgcccgcca caaaaaggcg acctcgccgc
    21601 tggcgcgccg cttctatgaa cagttcatgc ggcaaggcgt ctgcccgcgc tgccacgggg
    21661 cgcgcctgaa cccgcaggcc ctggccgtac gcttgacggc tcgcccctcc gacgcgcgca
    21721 agccgatcga attcaacatc aacgaaatct gccagctacc gatccgcaaa gcgctggaat
    21781 tcttctccac gctggttctc gacgacgtcc gccgcctgat agcggccgaa ccgctaaaag
    21841 aaatctccgc ccgcctccaa tttctgctcg acgtcggtct ggactacctc acgctcgacc
    21901 gcgccgctcc gacgctgtcc ggcggcgagt cacagcgcat ccgcctggcc agtcagatcg
    21961 gctgcgggct ggtcggcgtg ctctacgtcc tggacgaacc gagcatcggc ctgcacccgc
    22021 gcgacaaccg ccggctgctg gattcactga agcgcctgcg tgacctcggc aacacggtga
    22081 tcgtcgtcga gcacgaccgt gacaccatgc tggcggccga ccgcattgtc gacttcgggc
    22141 ccggccccgg cgtccgcggc ggccatgtcg tcgcccaagg cacgctcgac gatctgatgg
    22201 ccgccccaga ctccctgaca ggcgcctacc tcagcggccg acagaccata gcggtccccc
    22261 aatcccgccg ccccatcaag cgtaatcaca gcgcaaagca gagccggccc gnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncctgataca gaaagcctgc ctctcacaaa
    22441 tactttctcg gcgcctcact ctgtatccgc gcggctggac gcggttatcg gcacattatc
    22501 aaaacccggt gcaaatacac tgatcacctt gagcaccttt ttctggcgcc tacgcggagg
    22561 ccgtttttat cggccaacac tgatcatctt ctcataaccg tgatcacctt cgaacaccac
    22621 cgcaacgcat tgaatcccac cgcatcgcat ttttttatga gaccgcaagt catccaacgg
    22681 gcggagcgtc agttgtgccg gacggagtgt acgacctacc gccccgcgtg tattggacat
    22741 cgcgcctgcc tgcacgacgc aaaaccgggc ccggcagcgc catcctgctg caccgtctat
    22801 cgccgacccg ccaatcagcc gataccaaaa cgcgcaacat ggtttaggcg ggtgaactat
    22861 ggcagtgact atgatctgcc ctaatctgaa gtgccaacga accgtcgtag cgccagactc
    22921 cacgcgcggc aaaattgtcc gctgcgccca ctgccatcaa ccttttatgg tccccgtcaa
    22981 aagtgtcgag ccggtggccg caccgccaga accggccgcc aaagagggca aaaagtaaac
    23041 ccggccaaca ccgggaatac gcattggccg gagcccgaat gcaaacccca gccgcaccag
    23101 cttcgcgagc cggttctaaa acacacactc tcccccgaac cagcctgtcg cgcatcaacg
    23161 gcctcgccgc ccagttcgtc tcagagccgg agatgattct ggcttacctt gagccgctgc
    23221 cgcccgactc gccccagctc ccggttatct gcgccgcggc ggacaaggcc gtgcagacgc
    23281 ggccggacta cgcggacctg cgctacttcg ccgcttacgc cgccttccgg gccggcgacc
    23341 tgccacacgc acgcgagctg gtcgattgcg cgactcgctt gaatccacaa ttcaaggcgg
    23401 ccctgatcct ggccggccgc atctgcctgc aacagaacga acatgcagcc gccctgacca
    23461 acctgcagca agccctcgcc gcgggcgcag actaccccga tgtctacgtc ctgctcggcg
    23521 atgcctggca agcacgcggc gacttggttc gggcggccaa ggcttatcgc caggcgctgt
    23581 gtaagaatca caggctgacc gaagcacgcc gcaagctggg cgcaatatct gccgggcgaa
    23641 acgggggacc ggtatgagtt acctgctcga aacgctggga cgcggactgc tggtcgaact
    23701 gctttccgcg ttcgagaacc agttgccggg cttcagcgaa gaacccgagc cgctgcggga
    23761 ccgccttgcg gcctcgccca actcggcgga cctggcggtg cgcttcggcg tttcttgcct
    23821 gcgccggcgg cgcctggccg aagcgcggca ggccttcgaa agcgcgctcg aacttccgcc
    23881 gccctcggcg cagcctgcgc ttggcctggc ctgcgtgtat gacgaactcg gccaattgga
    23941 tcaggtgctg cgctacctgg cgatcgccgc cggtcacgac cccaacgacc cggccatcgt
    24001 ctttgccatc gccttcaccg aggagcgccg cggcgacacg gatgcggcca tcgccaatta
    24061 caagcgcgcc ctggaactct gcccgcgcct gcgcaacgcc tgggagcgcc tggcagccat
    24121 cgcgattatg cgcggccact ggcgcgaggc gaccgcgtgc tacgagaaac tggccaagct
    24181 cgaaccgggc gacctcgaca tcctgcttac attggcgacg ctctgcctgc agacggggcg
    24241 caccgcggat gccaccgagc gctatcagca ggctctggtc attgagccgg acttctcgga
    24301 cgagccgctg gccgagaccg aagacttgct ggccgagggc cggctgcaag aggcgatcga
    24361 gaccctggag gcgctggtcg agcgataccc gcacgcagcc ccgttccgcg tacacctggg
    24421 cgacctctac gccaaggccg atcgcggggc cgacgccatt gaacaatatc gcctcgcgct
    24481 ggaaaaccag cccggcctgc tcgaagctac ggtgaagctg ggcacgcagc acctgcgact
    24541 cgaacgctac gtcgacgccg cgcagacttt caaccgggcg gtcgaactga acgaccggct
    24601 catgctggcc ttcgtcggcc tgggcgtttc gcagcacgcc aacggcagcg agcgcgaggc
    24661 gctggccacc ttcgacctgg cggccagcct cgagcccagc acgaccctgc ttttcgcgga
    24721 aagcgcgcgg ctgcacttga aggccgagcg ctcgggcgca gccggtgggc tgccggaata
    24781 ctccgatctg gatgatgaag aggaggccgg cgccgcagac gacgcagacg agcaactact
    24841 gcaagtggcc ttacaacgcc accgccaggc cctgcttcgc aatccgcaac acgcggattt
    24901 gcactaccgc tacgggctgc tgctgcgtca aatgggcgac tacgacgaag ccatccgcgc
    24961 gttccgcaac gcggtgcgca tcaaccccaa ctacgccaag gccctggtca agctgggcat
    25021 cctgctgaag gagaccggcc agaccgacga agcggtggag gtcttccgcc gcgctctggc
    25081 actgaacaag gaatacgtag acgtccacta ccagctcgga ctgctgttcg cgcagcgcaa
    25141 ccgttttgag ctggcggttg aggaatttga gcaggccgtc gccggcaacg gtcagaacat
    25201 ggtattccag gcccagctcg ccctggcgct tcagaacata ggcatgctcg accgggcggc
    25261 ggccacgtgg cgctcaattt gcgagctatc cacggtagcc ggcgaaccgg acaagatgct
    25321 cgaacaccgc acgcgcatcc tgcggcaagt caacaaccgc gagtagccgg gcggtcaggg
    25381 gttaagacgc ttgggctatt agctcagccg ccggtcagca attccacgaa tgcgtcgata
    25441 tcaaacacat tcacctcgcg gtcatagttg cagtccgcat tcaggtgata gcattccggg
    25501 tatgcctcgg catatgccgc cggatccgtc agcgccatta cgaacggatc gatgtcgaag
    25561 acgttcaccg ggccgtcgca gttcaggtcg cccggattgg gcgcacgcag ccaggtcgcc
    25621 cgtataccct ggaccaccca gtcgatagtt tcaacttcac acacctgcag cacctcgccc
    25681 cactgcggca cgaggtcgac gaagaacgac aggtacgtgt cggggatatc gatcaggccc
    25741 ggctgccaac tcgtgccatc aaacaggaat gacacctctt gccacaatcc gccctgctcc
    25801 ccaagcatta cccccaccgc ccgccggccc tggtagaacg ccgcccgcac ccgcgtttgg
    25861 tatgggttgg cgaaagcgta cagcacttcc ggcggctgcc agcccgcgcc cgcgtcatag
    25921 cggaatgagc agaaccggct tagcccatca aaccgcgaaa aaacaccgaa aacatcgccg
    25981 gccgcgttca ccgccgtccc gccgaaattc tccacgtccg tcacggtgta gggcgccagc
    26041 cgcaccgccg gctgccacac tccgtcgacg ttccggctgg catacgccgt gtatggcgtg
    26101 ccccagaaaa gtgtgacatt accatcatcg tcgactacgc ccggaaagcg cgacgcgttt
    26161 ccggcgacgc cataccacgc agactcgctg cccggcagga actcggccgg cccccaagta
    26221 cacgtggccg ggtcaaattt gtgcgcgtac acgccgaccg gccctacgtc tgccaccagg
    26281 taaaccaagt acatcgtgtc ggtccgcgga ttgctcagga tcgtcggcag caaaaccttg
    26341 tagcccaacg tggacacgcg ctccggcgta ctccaggcgc ccgtagccgc attgcgaatc
    26401 acagtccacg cggccggcca attcccgggg gacaggtcga acacgacggc cacgttgccg
    26461 gcctgatcga cgccggcctc gatggcctgg aaaaaggtgg aggtagaata gagcgtctgc
    26521 gccggctccc agccgccggc cggcgtatag cgcagcactt tgaggtgata gccgccttcg
    26581 cggaaggcga cggtgatgtt ctcgtcactg tccatgacgg cctcggtggc cagtatgtat
    26641 gaagttccgc catggatggt ctgcactggg ccccagtcgc cgctgtggcc gaccgacttc
    26701 cgcgactgca ggtcgcaatg cgtcccctcc cagtgcgtca ggatcagcca cgcattaccg
    26761 gccctgtcga gcagcggcgt attgtgatag tccgcaatgt ccggcggcgg cggaggcgcg
    26821 atctcgaaaa gcggctcgtc ccaggccggc tccggccagg catccgttgc gccaggcgcc
    26881 aacgcttcgg ccggtgcaaa ctgcgcgttc atgcacagca accgcccggt cgcatcaatg
    26941 agcaagccgt tgctcaatag agcgacctgt ccgtcccacc tggcagttgc accaccccga
    27001 tcgttcgcca ggcgaatttt ccagagggcc ttaccgtccg gctgcgcggc ttgcaactcg
    27061 cccggactgc agaccaggcg caaatcgggc tccgcggcaa aagtcagaac agccaaacat
    27121 ccagcgcaaa tagtggtgac aatatgactc ttcactgaca tgcacgtctc tcttatctga
    27181 atgtgccacc accgtctgaa atgatactga aaaggggtga aaaatccaaa ttcctgtccg
    27241 cagcattcta aagcacgctg gcaaaaagac ttacgtctag ccctgggtcc gaattggcgg
    27301 tattcgacga cggtagcggg ccggcgctat atgtcggtgg agggttcaga cttgctggcg
    27361 aagttctgag cgacgacttc gcaatatggc gctgcgcgcc gaactacctg cccggcgacg
    27421 ccaattgcga tggggcagtc gacacgtttg acatcgaccc gttcgtcttg gcgctggtta
    27481 atccaaccgc ctaccgggcg gagttccccg aatgcctcat cgagaatgcc gacgttaatc
    27541 acgacggcac ggtgaatgtg ttcgacatcg acccgttcgt cgcgctgctg acgcaggcgg
    27601 gctgacccgg ccgggtaaca gacagggttg atgctggcgg aattacttat ataatttaat
    27661 ctgagccagc atggatttcc gatggaacga atggaacctt gaacatgcgt gccgccatgg
    27721 cgtaagccca cgcgaagccg agttggttgt ccggacagcg cgccgcccat tcccaaggcg
    27781 tgcgacggat gatcggtttt tggtgtgggg gcgaggaaca ggcggacgct tggtacaggt
    27841 cgtgttcctt attgattccg aatattcgat ttacatcatc cacgcacggc cactgactga
    27901 gcgcgagaaa cgcaggttcc gccagagatc atcatgaatc gcaccaagaa caagcccgag
    27961 aaggcgaaag cctccgacgc tgccaggctc caacaactga cggctgactt ggaccaggag
    28021 ttcgtggccg acaagtttgg ccccttggat gctgcatccc gccaaaagtg gcagcgggcc
    28081 aagcgcaagc gcggccgccc cgttcgcggc gagggggcgc aggtgatctc gatcagcatt
    28141 gagcgcggac tgttgcgggc gtgcgatgag ctcgcccgtc gaaagaagat cacccgcgcc
    28201 aatctggtcg cccgcgggct gcgcgctatc ctggcggctg agggggcggc ggtcctcact
    28261 gatgtcaggt gaatgcacct gacatcagtg aatcaccggc acatgagcat tgactccggc
    28321 tgaactgcgg tcgagtgata aagtgataag gtgatagggc gataaagggc tccggcaaac
    28381 cgtccctatc acccaatcac cttatcacct tttcgcggcc cgctcgctgg cgcgcctgct
    28441 gaccagggcg cttcgggcac ggcaccctgc gagcgtcaac tcgcacggcg ggcgggtttt
    28501 gcttacgatc caccaggccg acagtgattg gagcatggat gcccagaaca agatcccggg
    28561 catgcccgcg cttagagctt gggcatggca ccccgcatgg tagtccgcat ggcgccgcgc
    28621 gcgggttgaa atcgcaggac cggcgcgggc ggcaggccgg cagggtcggg ctgttcgccc
    28681 ggctcgacgc actggcccga aatctctggt ggacttggaa cagcacgccc caacggctgt
    28741 tcgccgcgct cgaccccgtg ctgtgggagg cgacgaatca taacccgatc gccacgctcg
    28801 cccgcctctc ccccgaccgt cgggccgccc tgctcaacga cgcggacttc gcaagcctgc
    28861 tcggacgcgc cgaggcggaa ctgaacgaat acctcaccgc ccgcacctgg ttccagcgca
    28921 ccgcaaaccg ccacgataag accctgcgcg tggcctactt ctgcgccgaa tacgggctgc
    28981 acgaagcgct gccgcagtat gccggcggcc tgggagttct cgcgggcgat cacctgaaag
    29041 ccgcctcgga tctcggcgtg ccgctggccg cggtcgggtt gctgtaccgc tccggttatt
    29101 accggcaatc gttcgactcc tccggcgcca cccgcgtcgc ctacccggaa tacgacttct
    29161 cgctcctgcc catcagcgac actggccggc aggtcgccgt gccgctcgac aaacggctgg
    29221 tgcacgcccg catctggctg gtccgggtcg gccgcgttcc cctgtatctg ctcgatacgg
    29281 acgtgcccga gaatcagccg cgcgaccggg ccatcacgcg cgtcctctac ggcggcgacc
    29341 aggtgaagcg cattcagcag gagatcatcc tcggcgtcgg cggcgtgctg gcgctgcgcg
    29401 aactcggaca cgaccccaac gtcttccata taaacgaagg ccacgccgcg ttctgcaccc
    29461 tccagcgctt gcgcgagctg cggcaagcag gctggccgct cgaagccgcg gaggagcgcg
    29521 tctggcaatc aaccgccttt accacgcaca cgcccgtgcc cgccggccac gaccgctttc
    29581 cgcccgaaat ggtgctccgt tggctgcgcc cggtcatcgc cgacatcgga ctgccgcacg
    29641 acgccttcct ggccctcggc cgcgagcatc ccgagagccg gcgcgagccg ttctgcatga
    29701 ctgtactcgc gctcggtctg agccagcgca ccaatggtgt gtcgcgcatc cacggggccg
    29761 tgtcacgcgc gatgtggcag aacgtttttg aggcggcccg gccggaggac gtaccgatcg
    29821 gccatgtcac gaacggcgta catgcaccga cttggcttgc tccggagatt gaaccgctgt
    29881 accagcgcta cctgcggcct cgctgggtcg gcgccggtcc tgaagacgat tggtggaaac
    29941 acgccgcacg catcccgccc gcagagctat ggcgcgtccg cacgctgctg cgccgcaagc
    30001 tcatagcttt catccggcag cgcttacggc agcagatcga ggccggtttc ggctcaccga
    30061 aagagctggc cgccgcttac gagagcctgg acgagaacgc cctcacgatc ggcttcgccc
    30121 ggcgcttcgc cacctacaaa cgggcgacgc tcatcttccg cgacgtccgc cggctggccg
    30181 ccattctgaa cgacccacgg cacccggttc agctcgtatt cgccggcaaa gcccacccgc
    30241 gcgacgtcgc cgctcaggcc ttcgcccaga ccgtgtggcg ctatgcccgc cggcccgaac
    30301 tccgcggccg catcgtcctg cttgaagaat acgatatgaa cgtcgcgcgc gcgctgatcg
    30361 ccggctgcga cgtctggctc aacaacccgc tccgtccgca ggaggcctcc ggcaccagcg
    30421 gcatgaaacc cgccctgcac ggcgggctga actgctcggt cctcgacggc tggtgggccg
    30481 aggcattcaa cggacgcaac ggctgggcca tttcgcctga taaggacgtg gacggccggg
    30541 aagccgacgc gtgcgccgcc gctctcgaac agtgcagtct cagtcgctcg cattcgcggc
    30601 gcactgccgc tcagcttgac cggcgtgacg ccgaaaccct ttatgaactg ctcgaaacgc
    30661 aaattgtccc gcagttctat gaccgggatc gcaccggcat tccacagcaa tgggtggccc
    30721 gcatgctgga gtccatgcgc agcatctgcg gccagttcag cgcagcgcgc atggtcggcg
    30781 aatacgttcg tgattattac ctgccggcgc tggagggctg acgggtcagt gcagcctttg
    30841 gacgaactgg ggttcgcgca atgctattcg acatttctcc actggttacc gaatcgatgg
    30901 cgatctggcc aggcgatgtt gtcccgcacc gggaggtgca tgcggacttt cggcacggcg
    30961 agcgggtcag cttgtctgcg tggcgcagca gcgttcacgt gggggcgcac gtcgatgccc
    31021 caagtcacgt gaaagagagc ggcgccgcga tcgatcaact gccgctcgat acgttcgtcg
    31081 gtcccgccca ggtgatcgcg ctgcacttgc cgccgcagac cgccattgag cccgaacatc
    31141 taccggcgcg gctggctgcc ccgcgcgtcc tactggcgac cggcagcttc aattacacaa
    31201 aaccatttga tacccggttt atcggattat cggcggcggc ggtcgattac ctgcacgagc
    31261 gcggtgtgct gctggtgggc atcgacacgc cgagcgtgga cttgttcacg gctacggatc
    31321 tgccggcaca cgaggcactg gcgcgacatg aaatgctcgt cctggaaggc ttggacctgt
    31381 caggtgtgcc ggagggtttg tacgaacttg tagcgctgcc tctgcgcctc gcgggattcg
    31441 aagccagccc ggtgcgtgcg gttctacggc gctaaattcg cagtgaacga acgactagtg
    31501 ggttgataag tcatttatcg caccgcctct cgctcacaca ggggttacgg tgattgtgcc
    31561 gtcagcaggc gataaaattg accctggcga attcttgagc tatactttcc gttatccggt
    31621 gctgaacagc gtatctccat cgtgggaggg agtgctctcg cgtgccttgg ttgtgtgtgg
    31681 cgaccgggtg ctagtcgcag ccgttgatgc ataaccctct tagagacaaa gtcttagaag
    31741 cggtcgatct ggttgaggtt gtaagcgagc atgtctcgct acaacgtcgc ggcaaggagt
    31801 ttatcggcct ctgcccgttc catccggacc acagcccgtc cctccacgtc agcccggcca
    31861 aacagatttt caagtgcttc gcctgcgggg ccgggggaga tgcgattaag ttcattcaga
    31921 tgcgggaaaa gttagggtat cgggaggccc tcgctttgct ggcccagcgt gccggcatca
    31981 gtttgcaggt ttcagaagcc gaccgcgccg ccgatgcctc gcgcgagcag tcacgcggag
    32041 ttctatcctg ggcgcgcacg cattttcaac gcaatttagc agccgcgcgc ggcgccgcgg
    32101 ccatggatta cgcgctgaaa cgcgggcttt cgcgcgagac gattgagaaa ttcggactcg
    32161 gcttcgccgc ggccgcctgg gacgacctgc tgatggccgc tagacgcgct ggggtggcgg
    32221 ccgagttgct gaaacaaaca gggctggtaa cggccaacga atcggggaag gtttacgacc
    32281 ggtttcgcaa tcggctgatt ttcccgattt gcgataacct aggtcgtccg gtcgcgtttg
    32341 gggggcgagc gctggacgac gacccggcaa agtacctgaa ttcgcccgaa acggcgttat
    32401 ttagcaaatc acgcgttctt tacggcttgg acgtagcccg ccgagccatc gcggaacggc
    32461 gggaggcggt ggttgtagaa ggctatatgg atgcggcttt gctgcatcag ttcggtttcg
    32521 ggcacgtggt ggccactctc gggaccgcga tgaccgattc ccacctgaag ctgctgcgcg
    32581 gagtcgcgga tcggatctac ctttgtttcg atagcgacca ggccggcatg cgggcggcgg
    32641 accgcgcggt cgaggtttcg ctggtcggcg gagtcgaggt tcgcgtcgtc atgctggagg
    32701 gggccaagga cccggccgac ctggtggttc ggtcgggcgg cgaagctttt cggacggcgt
    32761 tggcgaaggc ggtcgacgcg ctacaattca agtggttaca gacagttagg acgttcggag
    32821 atgggggctt gggttcccgg cgagccgccg tggagtcgct gattgagttc attgccagcc
    32881 tttcgccaaa cgctggaccg ggcccgttgg agcaaggacg gctggtgcgt cgactgagcg
    32941 aactactcgg cctgcccgtc gaggccgtct acgagaagct ggccgccggc cgccggcgca
    33001 aggttactgg cacagcggat gccgccagcg aggacagccc gtacgcgatg gccgtacgtg
    33061 ggctgccggc cggccttgtc acggcttccg aggaagcttt ggggcttctg gtggccgaga
    33121 gccgatgcgt gcccttgata aacgaagacg tcgcgtatgc cttcagcttg tgccgtccat
    33181 gggatcggtt gtacgcaata atccgcgaat tgtcggacgg cggggcgtac tcccaaacga
    33241 atgttatcga acggtgtcag gagatagacg tatgtgatct ggtcggccgt tgcagcaggt
    33301 gcacggccgg cattgaaaac cgggaagaag cctttctccg cgtacgcgac cgaatccgtt
    33361 tggaattgga tttgcttcgg agcgaggaat tggaccggaa ggcaggggct gagaagctgt
    33421 cggcgagcga tgaggttttt gatctggcgc ggggtcagac ccgtggccgc gatttcacct
    33481 tcgcagcgtg caaatgttat gccgctcgac cggcttcgtg atccatcctg ccgaagggcg
    33541 ggtggcaaag cgtcccgcat gcgagcgctg ccgggacggg tgctgcgttt gaaaaggtcg
    33601 cagcatccga aacgaatccc cttgcgggat gaattggaga accttaatga tcgtcggaaa
    33661 gcccgacatt gacccaatcg agcaaagcgt aaacgagctg attgaggcca gctttaagcg
    33721 aaagtacctt acttgggaag agctgaatga gactttgccc gatgaggcca tctcccccga
    33781 aaagctcgaa agcgtcctga accgcatcga gcaggcgggc atcaagctca tagacgagat
    33841 cgaagccgag aagatgcgcg acgcggaccg caaacgcgtc agcgtgctgg agggcggcga
    33901 tagcttggac gaggtgaccg aggtcgacct caccgacgcc gccgccaagc gcatcgacga
    33961 cccggtccgc atgtacctca cccagatggg tgagatcccg ctgctggcgc gcgaagagga
    34021 aatcgcgctc gccaagaaga tcgaactgac gcgcatggct ttccgccgca aggtgctcga
    34081 aaacgacttc tgcatgcagc aggcggtcga caccatgcag gccgtggccg acgggcgcat
    34141 gcccttcgac cgcaccatga aaatctccac caccgagagc atggccaaga gcacgatcgt
    34201 caagcgtctg ccggccaacc tggccacggt gcgcaagctg ctcgagttga atcaatccga
    34261 ctggcagacc gtccgcgata accgcacgcc ggccgccgtc cgcaagatgg cccgcgaccg
    34321 catcgcgcag cgccgccgcc gggcggtcac gctgctcgaa gagctctcgc tgcgcaccag
    34381 ccgcattacc cccatgatgc gcaaactgaa cggtatgacg tgtaaaatgg aggccctgca
    34441 gcacgagctg aagacgcgcg gcaaaaccct tccgcccgaa gaagtcgagg cgatgaagga
    34501 cgaattcaac ggtctggtcg acctggtgat ggaatttcct gaagacctgc ggcagcgcgt
    34561 caacgccagc cagcgcgtct tcggtgagta cgaagaggcc aagcggcgcc tggcgggcgg
    34621 caacctgcgc ctggtcgtct cgatcgccaa gaaataccgc aaccgcggcc tgtcgttcct
    34681 ggacatcatc caggagggga acacgggcct gatgcgggcc gtggataagt acgaatatcg
    34741 ccgcgggtac aagttcagca cttatgcgac ctggtggatt cgccaggcga tcacgcgcgc
    34801 catcgccgat catgcccgca ccatccgcat tccggtgcac atgatcgaga ccatgagcaa
    34861 gctgcgcaac atcaataagc gcctgcttca gaagctgggg cgcgagccga cgatcgagga
    34921 gatcgcggcc gaggcgaaga tgccggtcgg cgaggcgcgg cgggtgatga agatcagccg
    34981 ccacccgatc tcgctcgacc gtccggtggg cgagagcgag gattcgtact tcggcgactt
    35041 catcgaggac gaaaaggccg aaagcccggt ctcgaccgcc tcgaacgaca tgctgcgcga
    35101 ccggatcgaa gcggtgctga agacgctgac ctaccgcgag cgcgagatca tcaagttgcg
    35161 ctacggcatc ggcgacggtt acacctacac gctggaagag gtcggcaaga ttttcaaggt
    35221 gacgcgcgag cgcgtgcggc aagtggaagc caaggccatc cgcaaactgc aacacccggt
    35281 ccgggcccgc aagctccagg gcttcctgga caacgcgcgg tttggcacgg caagcacgta
    35341 gcgtttaggg taatagcgac ggatccaggg atctaaggat cgagggatca agaatgcagg
    35401 gccggggcgt ggcggcccgg aatgccgcca gcgccctacc acccgcgtag tcgtccagcg
    35461 aaaccttgag ggatcgaggg atcgagggtg caagggggca agagggcaaa gaatccgagg
    35521 ccgaagtgcc agcgcgggca acccgatccc ccgcaccaag ccccctgcgc aatccgaggc
    35581 cgcagcgcca gggtccaagg tagctacgcg aactgcgcga ggcgggcata gccgaaacgc
    35641 ggacgcgctt aaaaacgcgg cttttgttcg cggcggggga ggcggtctca taataggcct
//
LOCUS       DAOSYS010000007        34611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1025_length_34611_cov_5.324951, whole genome shotgun sequence.
ACCESSION   DAOSYS010000007 DAOSYS010000000
VERSION     DAOSYS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 34611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34611
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1025_length_34611_cov_5.324951"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1692
                     /locus_tag="PLU99_00925"
     CDS             <1..1692
                     /locus_tag="PLU99_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOO15644.1"
                     /translation="VPASAPAPAPAKFQPPEWAQHAIWYQIMLDRFRNGNPDNDPDRV
                     RPWTSEWFTPSPWEGRHGREFYESAYRRYYGGDLDGLERQLPYLRDLGVNALYLTPIF
                     KAASYHKYNQTSYIHIDDHFGTKGDYDKVAAQEDLLDPSTWQWTESDRRFLKFLRVAH
                     SMGFRVIIDGVFNHVGTAHPAFQDVVAKRTASRYARWFDITLWGPLSYRGWWDHAELP
                     VFKKAPEGYAAPEVRQHIFDITRRWMAPDGDVSAGIDGWRLDVPNEVPLPFWLEWCPF
                     VRSINPQAYITGELWRRADEWLDGRSFDAAMNYEFARAVVAWVFDRRQKISVHEFDRR
                     LAELRQAYPPAITRVVQNLMNSHDTDRLASMAFNPDRPYNELNRPQAEGVHYNNAKPS
                     ADAYARARLAAFIQMTYIGAPMIYYGDEVGMWGAADPTCRKPMLWKDLEPYERPEENH
                     VMDEQLEHYRRIIALRNAHPVLRTGAFETLLCHDQADVWAFLRGDGGEQAVVAINASH
                     ESRIIAIPLPAGSPEHWNVVYGTPRVVQVSNGALPVEIPPLDGIVLSARTDKAGI"
     gene            complement(1700..2788)
                     /locus_tag="PLU99_00930"
     CDS             complement(1700..2788)
                     /locus_tag="PLU99_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOO15645.1"
                     /translation="MLIAQCPACHARYKVPDEVAGRRAKCKKCGQPFQVPEPPPDSGR
                     LSLTAITPPGEGSTVRLRVDKPQRTAKAEKGVRLRGEAEEKQEAAPGVWGPFIRDLAS
                     CLWAPVRPVNLPPFLIAWFLLTIAKPGLWSFLICAPVGLAIWAIINGWYLSFQFNTAV
                     NATDGKAELPTFTFGSGFVDDVLVPLLKMVLTQLGALLPAIIYVAAVILARGGSLDYA
                     QGVGPGVGPLNIYLAAVGSADGGAAPMVALALLAVGLTVWPILVLATAVGGAPAAFRF
                     GTMARGLVRTVPAYALVLLTVYAAAAVGIVMGMALGRSLPDTSEANVTRIVVYPALIA
                     LADVYLTIIAMRALGLYYHHFKQKLALV"
     gene            3127..3315
                     /locus_tag="PLU99_00935"
     CDS             3127..3315
                     /locus_tag="PLU99_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOO15646.1"
                     /translation="MRMFTPRHIAALRRFGQEDDGPTATEYAVLLALIVLVILTAIVP
                     IGTSVNDAFRFMVSQLGG"
     gene            complement(3408..4457)
                     /gene="xerC"
                     /locus_tag="PLU99_00940"
     CDS             complement(3408..4457)
                     /gene="xerC"
                     /locus_tag="PLU99_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOO15647.1"
                     /translation="MQNDVVVQQNWGPHAGSDGSTMDEQASLIQQFINYLRAERHFSP
                     HTSKCYTADLAQFCQYLVGQKCAPHDVSTGGNNGPAERLPATAEIGGEAMNRRLLEVD
                     TDVVRGFLSELREKNYCKSTVARKLATLRSFYKFLVRRAYLSSNPVAPIRTPKQDKRL
                     PKCLEIEQIERLLSNPDTTTLLGARDRAMLETLYSTGLRVSELVALDVSDVLLDTSVI
                     RVRGKGKKQRMIPLGPGAVQAILHFLDMRRNDSRSGNFDAEALFINKHGRRLSTRSVR
                     RKLDKYLLEAGLDLSVSPHTLRHSFATHMLRRGADLRSVQEMLGHQSLSTTQIYTHLS
                     GERVRKTYDEAHPRA"
     gene            complement(4681..5439)
                     /locus_tag="PLU99_00945"
     CDS             complement(4681..5439)
                     /locus_tag="PLU99_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15648.1"
                     /translation="MLLTKPRIRMGTAFFALVAATGVSGGCASAHPGNNTHQPHKPIP
                     PRQVIVVAPVLNLSNSTEFDTLKVTDAVASEAVGFPGIGVVPVNLVLAALAERGTTRV
                     ESPAEAVELARRFGAQATLVTAVTEYRPYDPPVVGLIMQWYAVPDAPLSADGQTALRP
                     DSNACETAAALSVIPTGPQTQLQRVFDAAHEEVRDEVKSFARRRDGHLSPYGWQRYIK
                     SQELYVRYCCWSLIRSILAVEKSEWAVSAAEARL"
     gene            complement(5766..8201)
                     /locus_tag="PLU99_00950"
     CDS             complement(5766..8201)
                     /locus_tag="PLU99_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOO15649.1"
                     /translation="MLVAVLLAPLIALFQTAPSANPVAPFAPSPGSALEAALSSGLPS
                     ATAFEPAAAPDSGPGFQARLSFAQTSVQPGGEADLAIELRFAPGWKIYHPLILDTGLP
                     TEVEFELPKGVTVGELRFPAPTWSTIAEGELKQEFLGLESPVVMLTKVRVAPDTPAGP
                     LTLKATASALACKGLCVPAEAEATAVLPVLTDPPAPANQQLFEDARRNLPQQFSEADN
                     IAGSRVLAAHTQLPVGERGTIVAAVRVKSGHHIQDRDPGVEGLIPSRLYIEHVGGLKF
                     LDQVWPQPYVKEYPELGKVRELGGDFVIQAPFEVEQDFEPKKLRLRALFEYQVCADTG
                     ICFPSNLAEGYIEFEVVPAGAPAVASTDPILAKLGEVGEASPAASPSRAGTTAIPPNL
                     LWVFLAAFAGGAILNVMPCVLPVISLKIFGFVQQAHEQRGRIFRMGLAYTAGIMASFL
                     LLAILMVYAGLAWGGLMQRPEFLIGLVAVVFAFALSLLGVYEISLPGRITSAAGEAAA
                     RGGYVGAFMNGILTTLLATSCVAPLLGTAVGVLMQLPNPYLAGAGIMVVGAGLATPYL
                     LLTAFPSWLRFLPKPGPWMVTFKQVVGFLLIAVVVWLLSILMKMIDHAMLQGTLGLIC
                     AIGFGCWLIGRIRLSDGTGRWAAHWSLALLFAGGGGFLSFWVFSPRVERIPWQEWKPG
                     MAQRLAAEGNTVFLDYTAEWCPNCKTIEAVVLNSQEVAAQFAKFGVYPIRVDFTTKDS
                     EIQEELRQHGRSGVPTYVMIPAYKPNEPIVLPEMPSGETVLEALRQAGPSKQRPEFWS
                     TPPNTQPTEAVCR"
     gene            complement(8441..9154)
                     /locus_tag="PLU99_00955"
     CDS             complement(8441..9154)
                     /locus_tag="PLU99_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO15650.1"
                     /translation="MTAQSKPVRSYYLDPAWPLWVRRQNQRVIDCERCPRLREFCRTI
                     AALRRAAFREQTYWGRPVPNFGDPAARLLVMGLAPAAHGGNRTGRLFTGDRSGDWLFR
                     ALHRAGFANQATATSIDDGLELENAFVTAVIRCAPPQNRPLPEEIRNCSDYLDETLRR
                     VPWRVLVALGGVAWVNAARRLAVRPGPFRHGAEFALPEGRVLLASYHPSQQNTFTGRL
                     TEQMFDAIFRRARRLVRES"
     gene            complement(9151..11766)
                     /locus_tag="PLU99_00960"
     CDS             complement(9151..11766)
                     /locus_tag="PLU99_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15651.1"
                     /translation="MRTEALCLWLCFSAAPLLNAQTPASRNAGQVNDLRIFADTPYES
                     LLTSKPAAVTSGILDLIGSQNCLVLLKRGPEFLTLIDPRGYDVAWANVTGIRGLLGEH
                     LNPVALTWFDWPLRDGDPGVLVLAEDGAALYLFDHRLRQLGDGPLVRVPQMRGRAVAL
                     GPRGHIFVADAGIPGIRVFHAAGGELLTYGKRGAVDGDEHADSNSAGKFNEPCGILVE
                     PQGDVLVMDRGNCTYAVCKYEPRPNRMKWTATGPWLPGVDVRFDDAGRVLLAGPVESN
                     TAAGALRVMNFSGAAERVFTGAALGGLSARLRVCQGPDGKYYLAEPEKDRILIVPPDF
                     VEKLPQFEWTRDGGVKLTMTKVDGSTVTTVSRERPPKDGGRILVRQSEPVCESWPPAL
                     VDELVAYSLPPAPPRGKTYVIDMPVLVAVFAKAIDENGALTTIEPTGVIERLQRELET
                     DRLFYWRNSYGTLNKQFEFMLIDDAEPRIADGWIEPTAARTLVNQVRQKRGLPPIGPD
                     HSLVAIHPMSGFDPAFTDDIGTVDGGGLTIYAYSSYGLWNNGQAWLMAHEWGHQLDDF
                     FQKSGFDDWWSNHPDGTLHIGRYGEHWDCNAFLCRRADKMNWLRLRYGTPRLVDDRDE
                     DGLADDDPTLPLDESRFGSDSWEPDSDLDGLSDLQEMAAGAFSSTNPLKRDTNGNGTS
                     DGHEPYPQFAVKTAIARADEQLMQRILAGEPPEPLLEKLGRISSAWCDTDVYAVYTPE
                     TLTLLFTLRKPVRRVFAPIDFNNDGWFVGSDQVYASAQLEWPAGGQPRLRGAELCEAA
                     ISSQAGGPILLLKISVPAAREPLRPGSSVALCVRVENGAGCVAFLLDPWQLLSLSLRE
                     SGALERLEQAKEVAG"
     gene            complement(11986..13059)
                     /locus_tag="PLU99_00965"
     CDS             complement(11986..13059)
                     /locus_tag="PLU99_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOO15652.1"
                     /translation="MFHHVKELQFNARVSKPDPHFAKLLLQQFGGPNGELKAAMQYFV
                     QAFGVRKSYPDKYDLLMDIATEEFSHLEIVGATITMLLAGVNGELKNAVERCELNKVM
                     RGNGPREELIHLAATNPQFLLESAGGPTVTDSVGTPWSGTYVNANGDLTVDLRSNIAA
                     ESRAKIVYEYLLQFTDDPLVQESLRFLMTREIAHFQMFTAALGSIDPNFPPGVLQGDP
                     RFTHTYYNMSAGEDARGPWNQGRGVWHQGEWAYIPEPIRQVHETEGQLRQPIQGSTLT
                     EERVSKIDQELARRRYQETSPAAAGGHEQQWSSYDGAGAGRPAAPPQAQPVTAASASG
                     PTGTHPGTQPGSRPGGQPPKPGK"
     gene            complement(13398..13544)
                     /locus_tag="PLU99_00970"
     CDS             complement(13398..13544)
                     /locus_tag="PLU99_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15653.1"
                     /translation="MNRKYLWLTAITLPGVLMSGCWAGLHQFAIHTFEVTDFVAALRV
                     LGLF"
     gene            14046..16151
                     /locus_tag="PLU99_00975"
     CDS             14046..16151
                     /locus_tag="PLU99_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15654.1"
                     /translation="MHVLRPSWRAAARLLWLVPVMLAAAPAARAESQADLELTRIALF
                     NSGVGYFEASAQVDGNATAELKFRTDQINDILKSLVVQDFGDGRVGVVGYAARDPIEK
                     TLRSFGVDLTGKPTLGDLLNQLRGEQVEVTGPRTLRGSILGVEKEQVLSPDGKTVQVI
                     ERLTVSTEAGLQQVKLAEVQSIRLLNEKIDNELQKALATLATAHDADKKTVSISFEGE
                     GQRQVRAAYLLEAPVWKTSYRLELGQDKKPFLQGWAIVENATENDWNNVRLSLISGRP
                     ISFRMDLYTPLYVPRPVEQMELYASLRPPEYEGQIADATGRWQAYADAETAPAEDAAA
                     LAPAPGAPTVRARRSKALEISGDGYMELQQEVPMALEGAGVQSVASAAEAGELFEYTI
                     QTPVSIQRQHSAMLPIVNEAVSATKVSIYNPATHAKYPLNGLELTNSTNLNLMQGPVT
                     VYDDNVYAGDAKLPDMKPGEKRLIAYALDLGTEVLVRQKPTPEELVSLRIAKGVLWLR
                     HKYVDQRDYVIKNKLERPREIILEQAYSEDWTLVQPKEPYERARGVLRFRTTVPAGET
                     VTYPVRLERLADQSLALTDLGLDHIRFYLKTKAVSPALQKALERVVALRTELDEASRN
                     RANLERSLAEAIQDETRIRENLNTLQKDADAYRRQLERFEKLETRIEQLRTQVAELRQ
                     IEEQRRKELQQYLLSLNVE"
     gene            complement(16223..18385)
                     /locus_tag="PLU99_00980"
     CDS             complement(16223..18385)
                     /locus_tag="PLU99_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15655.1"
                     /translation="MVKISENPAVLQQLAEDPQKVMNPASITPPVSGELQDAIRQIQQ
                     VPEVVALAAAFPDEMARLRQLYQSAPQQADELLANLQARYRAARLQGNQAWQQMLAQN
                     PQALEQYQNLVTEFTREQLKQYPQFPYVVVTDPQYYQAAPPNELLLAFAEDRKPPEPL
                     ASILNQWWQTYGISAVDQQVFSQAALAMPDATFLAALPSQERANMWRAASGAAPSDAR
                     LMPIIMQPLPDQPMDARLAFAVAENARLWGEPALTTQPPVAEAPQMQEGILPQYAEPL
                     EQQPGYAGQYPYQQYPGYEQYPAYEQYPAYEQYPAYDYGYAPDYAYSAPATTYYSPLV
                     PYYYSYPAVWPLAYSYYPSLSYYWPYSYYYSYGAPYFAFNFGYGHRFYKYPYFRYPYF
                     HRHPYFYGRYGRFYGDRGFYGGRFYGDRVYRRGAFDRYYGYGGRRYFGDRFYGSRGFY
                     GRGPFDGLRGYTGFRGGSFDRFGRTFDRGPTFDRRFTRDRFTVTRDRFGRTGTFDRRG
                     TTGTFDRRGTTGTFERGGAFDRRFAGDRFGPTGRGTLGPTDRRVRQGLVGERTIERRQ
                     FDRGAAVQRGATPGTRDFGTRLGPATPRGGTGGAIRSAPRGGAVPRTRSDAIRSMPRG
                     GAARSGAALRSSPRIGGPGRSGAALRSAPRGRGPGRSGAAMRSARPGGSPGRGSAIRS
                     APRGGGGGRGSAVRSAPRGGGGGRMSGMRGGGRGGGRR"
     gene            18748..19290
                     /locus_tag="PLU99_00985"
     CDS             18748..19290
                     /locus_tag="PLU99_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOO15656.1"
                     /translation="MPNVNTWHRIDLQCGDITRVKADAIVNAANSRLAGGGGVDGAIH
                     RAAGPGLHEACRKLGGCATGDAKITPGFNLPARYVIHTVGPVYRDGQSGEPELLASCY
                     RRSLEVAVENGVKTLAFPAISTGVYGYPFEPATRIAVQTVADFLSREPSIERVTFVFF
                     GKSDFEQAQRVLADLSRAAT"
     gene            complement(19494..20972)
                     /locus_tag="PLU99_00990"
     CDS             complement(19494..20972)
                     /locus_tag="PLU99_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO15657.1"
                     /translation="MRIALVNPITRRSQGYHTVGSYIPQLGLQVLAELVPDGHEVHLI
                     DEVFGPQFTDTHLTRSNYDLVALTSYTSGATRAYEIAAAARKQGIPTILGGPHASACP
                     DEAALHFDSVAIGECDEIWPQIVRDAAAGRLQSRYQGRLADLEATGLGRARQTLRPVN
                     GRYDVSALQTSRGCPVGCEYCSVTQFNGSHIRRRRIADILDEWNRTPEKFIFVVDDNF
                     FGVGRQHAEWAKELLRAIIKHGRKRLWFSQTTINMGDDPDGLALAYKAGCRGMLVGFE
                     SFNEQTLKRYHKVLNRNSLARYQELVNGFHRAGISVFGGFIIGSDEDTAETAADTALM
                     ATKMGVDIIQITNLTPLPGTKMYERFRAEGRLLATDYPADWERYTFTETVYQPLRMTS
                     GELDESIYELRHLAAKENWVLKRAIRTLVRTRSLTTALFVLGMNKGWKRMARIQAPHD
                     AERFGFSPPLSARLAKIGESFRLHLRSQLQPPAAPEPVAVQS"
     gene            21185..22600
                     /locus_tag="PLU99_00995"
     CDS             21185..22600
                     /locus_tag="PLU99_00995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO15658.1"
                     /translation="MARAHVETILAHESVPASLKEAIKRTGAWASFRPLSQALRGGVS
                     PRADAIVVIVPDDMRPVEHGLRVLFDRLADCPRAVLVMQPGSGVPAQLDHPATVPVTF
                     GSALGADELASRLATMVDMRPSLDSLHRSMLANRQQEENATAAYERQLRLARQVQQEF
                     IPENLPNFGPVKFSALFRPAEVVSGDMYDIHRLDEEHVAIALADATGHGIPAALLTVF
                     VKRALRGKEIHNGSYRLLPPTEVLERLNEELLEANLSECRFVAATYAVLNTRTMVLEL
                     ARGGTPYPILRRRDGRLELLRPAGSVIGVLPGARYPSQQVQLAPGDSLVICSDGVENV
                     TAPQISATALAETFSRAAAAVRAGTARERRRAAALVGAAACEDSGCFGGASSTTAVAE
                     IADEPAGSGDDEVPGIERPSRRASRMAPDEAMLASAWCATLRDEGPQAALEQLTVRYD
                     SLRRMGYPLDDLTVLTVQIQP"
     assembly_gap    22692..22791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22792..23910
                     /locus_tag="PLU99_01000"
     CDS             <22792..23910
                     /locus_tag="PLU99_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO15659.1"
                     /translation="GVNPGRTGCDLCLAAEQMILQTRKRLSAFFNPSAVAAGKPKDPK
                     RLIFTLNGTMSLNLIVNGMVGPGDHVVTTMVEHNSVIRPVNHRVRDAGATATYVVPDG
                     EGYIDPEDIRKAIRPNTKLVIVNHASNVTGVVQDVQAIGKVCRELGVPLAVDTAQTAG
                     VLPIDMTECNISFLAFTGHKGLLAPTGTGGICVADDAEIRGTIYGGTGVRSADPYHLE
                     EYPYRLEAGTQNLAGIAGLSAGLDWLEEHGMDKVRRHEMELMAELQNGLAEIPGVKIW
                     GTKRLDRRVATLSITVANYDPSDVGTILDAEHTVLVRTGLHCAPLIHDHMGSSPRGTV
                     RFSIGPYNTREHVAAAIRAVEQLASDRPATTQVQGCKL"
     gene            24096..25745
                     /gene="gltX"
                     /locus_tag="PLU99_01005"
     CDS             24096..25745
                     /gene="gltX"
                     /locus_tag="PLU99_01005"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOO15660.1"
                     /translation="MSEIVRTRFAPSPTGYLHIGGARTALFNYLLARRLGGKFVLRIE
                     DTDQTRNIEAADQKLMEDLRWLGLQWDEGPEVGGEFGPYHQGQRLELYRERAKYLLET
                     GHAYYALETREELEQMRATARARGEKGFRYPRPQRFPTEAEAEQARRAGRPVVIRFKM
                     PDRDFTVPDRILGDVHIAASELSDFVIIKGDGWPTYHFAVVVDDAAMHITHVLRGQEH
                     LMNTPNHMALQEAFGYPVPTYAHLPIIFNMNGTKMSKREKDKVVRDAAKAAGLDEQKV
                     MELAGVADPQLVRLWRAGETQLPGEALARLAKKLAVHVPEIEIHDFRKSGYLPEVLVN
                     FIALLGWSPGDSREKFTLDELCQVFTLERVGKTNARFDRDKLLAFNTMAFEAAEMPRK
                     LAAFRDWLSVNEPGPMTGLDDATLVRVLEACHGFRTFEDVDRKAGVLFAADEAYAYDD
                     KAVRKWLLKNEAAGLKVVQDLKALLAAATDWSPAALDDLIRRYAEQHNLELGKVAQPL
                     RVAVTGTTISPQISETLGLLGRRRTLARIERAVRVAESLAA"
     gene            25789..26166
                     /locus_tag="PLU99_01010"
     CDS             25789..26166
                     /locus_tag="PLU99_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15661.1"
                     /translation="MTGVTSVCDMRRYATGLLCAAGLALLAGCSGTLAGHWRMVEVQP
                     NRQVFCIDDVTFARDGSYSATTTLKGKTLEESGRYKFNGFKLTLWPDAGGCRQYDALL
                     KFNELEILDAKKTPKQMVILKKD"
     gene            26282..26575
                     /locus_tag="PLU99_01015"
     CDS             26282..26575
                     /locus_tag="PLU99_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOO15662.1"
                     /translation="MTNPPTGNRIRRHVIYSGRVQGVCFRAITQEISARYDVTGFVRN
                     LRDGTVELEAEGSAAAVDAFLAEIARHFQNNITHAQVSDVPPRNDEADFRIRY"
     gene            complement(26621..>27842)
                     /locus_tag="PLU99_01020"
     CDS             complement(26621..>27842)
                     /locus_tag="PLU99_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO15663.1"
                     /translation="GIGHYHLDKTTELECVECPCYCDVDTQRMARAAELFPNARPYQD
                     YRRMLEVERDNIDAVMIGTPDHHHYPATMLAMRMGKHVYTQKPLAHTVWEARQLTEAA
                     RRYKLATQMGNQGHALEGWRLVYEWVHSGAIGDVIETHTWTDRPIWPQGMSRPKEEEP
                     VPESLDWDCWLGPAPLRPFKGPAPEQNAGPYHPFNWRGWWDFGCGALGDMACHTMDGI
                     FAVLDPGSPAWVEPIAATPITDEAFPTCSMLRWYFPATASRPAFISYWYDGGLKPRCP
                     EALELERRLPDTGNLFLGTKGALLITGDYLDSPRIIPETLMRQIGKPPQMLERSPGHV
                     KEWVMAAMGQAPLDFPKSNFAYAGPFTEAVLLGNVALRTGRRIEWDAANLRVPNLPEA
                     NQYISKTYREGWRV"
     assembly_gap    27843..27942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27943..28263)
                     /locus_tag="PLU99_01025"
     CDS             complement(<27943..28263)
                     /locus_tag="PLU99_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOO15664.1"
                     /translation="MNQPANKTTRRTFIKTAATIAGAGIGGGLIGCGQPREKIQPVEA
                     AGPSAAQVEGMSARPVNAPAPLPAFHRGEYAPKLDKLRVAYIGTAGIGHYHLDKTTEL
                     ECVEC"
     gene            complement(28294..30108)
                     /locus_tag="PLU99_01030"
     CDS             complement(28294..30108)
                     /locus_tag="PLU99_01030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOO15665.1"
                     /translation="MRWSKYFVPTAKETPKDATAPSHVLMLRAGLIRQLAAGTYTYLP
                     LGYRTLRKVEEIVREEMNRAGAIELHMPVLHPISLWEETNRVQAMGDVLLRLGGPPGD
                     WRPNTVLGPTHEEVITDIARAYLVSYKQLPINLYQIQTKFRGEARPKSGVLRTREFLM
                     KDAYSFHADKDSLDAEYDNMYRTYCRIFGRCGLPYVAVQAESGAIGGDVSHEFMVLTD
                     AGEDQVVISENGDYAANLERATAAPLAAVPAEMKPLELVHTPGHGRVEDVCAFMGTTP
                     GEMIKTLIYVSVPADTLKDAPAPRPKRIVALVRGNHDINEFKLNRAAGMKVELADPQV
                     IEEVTGAAVGFAGPQGLIDKVDLLIVDNDVATMRNAATGANKTDYHVKNVNPGRDFPL
                     SGDKVVLADIRNVVEGDLSPTESGYPLRLRTAIEIGHVFKLGTKYSSAMQATFLTKEG
                     RPQPLIMGCYGIGVNRIIAAAIEAHHDANGIVWPMSIAPFHVLVLALDPRDEQVLRTA
                     VEIHDQLEAAGLDVLLDDRDERAGFKFKDADLIGIPLRVIVGKKSLNEGAVEFSHRKD
                     DVKQRFAPTEVVEHVRRLYTQEMEELMRAPLLPAANGD"
     gene            30426..30794
                     /locus_tag="PLU99_01035"
     CDS             30426..30794
                     /locus_tag="PLU99_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15666.1"
                     /translation="MNRLARSALGLSLVGLSLFGAAGLFGCVPGTLVDSAQNQAGAAI
                     PAGSYWVVETDAGLDLFQMPAYRGDAGKWQTLMGLPNWYTRDGLYSQDATGNWTLEID
                     GAETDLDSLLQVWDAPPPTS"
     gene            30955..32220
                     /locus_tag="PLU99_01040"
     CDS             30955..32220
                     /locus_tag="PLU99_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOO15667.1"
                     /translation="MQAQARDPLSVWVRLISGCLAAVLIEGSAFAQEASPSSAPVSTS
                     SAPTAVSQSASYWLRVSGDQVNLRSRADRNSLPVARLERDQVLQAVGMEYGWHRVYAP
                     EGVFSFVSGRYVDQTSETEGIVSTSSGALRVRVGSELSEVDPMQTEVQKLLPRGAKVN
                     ILGRRGEWLKIVPPDGVFFYVADDCVERITPEVATALRAKTARAPRAAGAVAATRPAE
                     GPDMSGPWGQRLALVEAAIEAEGRKDALEQSWTKSIAELRPIAEQRADPAVALLAQRW
                     ISRLEERIADQAALRTMRQLADAGQRNEAQLEREMEQIRKVRERADSPSELIARGQLL
                     ESFALRGGVSPPYRLIDPITRAVVAYLEFPLDSDLKPAEYLGLYVGVAGEKRTDEAFG
                     ADVITVSRLEVLLSRGPGGPQTAPARTSP"
     gene            32277..32453
                     /locus_tag="PLU99_01045"
     CDS             32277..32453
                     /locus_tag="PLU99_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15668.1"
                     /translation="MIKTRTRGRLLTGLAALTGLALAGCPLTDIQQAAILESTITGVI
                     GAIIQIAVQAAAGV"
     gene            32523..33590
                     /locus_tag="PLU99_01050"
     CDS             32523..33590
                     /locus_tag="PLU99_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOO15669.1"
                     /translation="MARARTLAPIVLVGILVLGGCTKRRSANIVQHPEWPYQDYQRIA
                     VLPFKACDRNAAEAARQAESLLIDNLTANGSFTVLSRGDLKDILTEQDLSRLADVADP
                     NTSVPAGMLQVAQAVVVGHITTFDLDQQKKERRQPRYAMDRNGRIIRDRNGRPRVVGE
                     DVLVEYRHVARVAGSVRVIDVATGRVLLSYTVPPVEKDDSRWNSPPSSSPEQLGIEVA
                     QEIATAFYRKLAPQTIRVKLGSDCLVVAGEYYEGEYEQLKKVPDDLDELLVVTRGLPK
                     SCDRNCFRMAVSPREGGEYIAEHEFIWSPSLGQRGEVLRVPVAKLRNNGGSQFTAKLF
                     AVGNDTPILARDFELVPSKAK"
     gene            33805..>34611
                     /gene="lpxD"
                     /locus_tag="PLU99_01055"
     CDS             33805..>34611
                     /gene="lpxD"
                     /locus_tag="PLU99_01055"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOO15670.1"
                     /translation="METTLIEACAALERHGMQPRLEGDPSRHIIGVATLEDAGESEIS
                     FLANPKYEKLLATTRAAAVVVRPDVTARNGLNLIRVDDPYAAITILIIELHGYRKHSP
                     PPRDAGSPNIAASAKIGANAQIHPGVTIAEDVTVGDNAVIYPGCYIGPRCRIGHNVLL
                     YPNVVLYEDTVLGNRVTVHSNTVIGNDGLGYAPVGGKWIKIPQIGTVEIGDDVEIGSN
                     CSIDRATLGCTRIGSGTKFSNLIAIGHGAQIGPDCLFVAQVGIAGSATVGR"
BASE COUNT         6337 a        10902 c        11004 g         6168 t
ORIGIN      
        1 gttccggcca gcgcccctgc ccccgcaccg gccaaattcc agccgccgga atgggcccag
       61 cacgcaatct ggtatcagat catgctggac cgcttccgga acggtaatcc ggacaacgac
      121 cccgaccgcg tgcgcccttg gaccagcgaa tggttcacgc cctcgccatg ggaaggccgg
      181 cacggccgcg agttctacga atcggcctac cgccgctatt acggcggcga cctggacggg
      241 ctggagcggc agctacccta tctgcgcgat ttgggtgtga acgcgctcta cctgacgcca
      301 attttcaagg cggcttcgta ccacaaatac aaccagacca gctacatcca cattgacgac
      361 cactttggga ccaaaggcga ttacgacaag gtggccgcac aggaggattt gctcgatccg
      421 tccacctggc agtggaccga gtccgatcgg cgcttcctca aattcctgcg cgtggctcac
      481 agcatggggt tccgcgtaat catcgacggc gtgttcaatc acgtcggcac cgcccatccg
      541 gcctttcagg acgtggtggc caaacgcacg gcatcacgtt acgcgcgctg gttcgacatc
      601 acactctggg gaccactgag ttaccgtggc tggtgggacc atgcggagtt gccggtattc
      661 aagaaagcgc cggagggcta cgccgcgcct gaggtaaggc agcacatatt cgacattacg
      721 cgacgctgga tggccccgga cggcgacgtc agcgcgggca tcgacggctg gcgcctcgac
      781 gtgcccaacg aggtgccgct gcccttctgg ctggagtggt gcccgttcgt gcgttccatc
      841 aacccgcagg cgtacattac cggcgagctg tggcgacggg cggatgagtg gctcgacggg
      901 cgctcgttcg atgcggcgat gaattacgag ttcgcgcggg cggtggtggc ctgggtcttc
      961 gaccggcggc agaagatttc ggtacacgag ttcgatcgcc gcctggcgga actgcggcag
     1021 gcgtatccgc cagccatcac gcgcgtcgtc cagaatttga tgaacagtca tgacaccgac
     1081 cgcctcgcgt cgatggcgtt caatcccgac cggccgtaca acgagctgaa ccgtccgcag
     1141 gcggaaggcg tgcactacaa caacgccaag ccctcggctg atgcgtacgc gcgggcgcgg
     1201 ctggcggcgt tcatccagat gacgtacatc ggcgcgccaa tgatctacta cggcgacgaa
     1261 gtcggcatgt ggggcgcggc cgacccgacc tgccgcaagc cgatgttatg gaaagacctc
     1321 gagccgtacg agcggcccga ggagaaccat gtgatggacg agcagctcga gcattaccgg
     1381 cggattattg cgctgcgcaa cgcgcacccg gtgctgcgga cgggggcgtt cgagacgttg
     1441 ctgtgccacg accaggccga cgtgtgggcc ttcctgcgcg gcgacggagg cgagcaggcc
     1501 gtggtggcca tcaacgcttc gcacgagtcc cgcattatcg ccataccgct gcccgccggc
     1561 tcgccggagc attggaacgt cgtctatgga acgccgcgcg tggtgcaagt gagcaacggc
     1621 gcgctgccgg tcgaaatccc gccgctggac ggaatcgttc tgtcggcccg tacggacaaa
     1681 gccggaatct agccggcctt cacaccaatg ccagcttctg cttgaagtgg tggtagtaca
     1741 accccagggc gcgcatggcg atgatggtca ggtacacatc ggccagcgcg ataagcgccg
     1801 gatacaccac aatacgagtg acgttggcct cactggtgtc cggcaggctt cggccgagcg
     1861 ccatgcccat cacaatgcca accgccgccg cggcgtaaac ggtgagcaac accaacgcat
     1921 acgctggaac cgtgcggacg agcccccggg ccatcgttcc gaagcggaat gccgccggcg
     1981 cgcctccgac cgccgtggcc agcaccaata tcggccacac cgtcaatcct accgccagca
     2041 gagccaatgc gaccattggc gcggcgccgc catctgcgct gccgacagcg gcgagatata
     2101 tgttgagcgg ccccacgccc ggcccgactc cctgcgcgta gtcaagtgaa ccgccgcgcg
     2161 ccagtatcac cgccgccacg tagattatgg ccgggagcag cgcacccagt tgtgtcagca
     2221 ccatcttcag cagcggcacg agcacgtcgt ccacgaaacc ggacccgaac gtgaacgtcg
     2281 gcagctcggc cttgccgtca gtcgcgttga cggcggtatt gaactgaaac gagaggtacc
     2341 agccgttgat aatcgcccag atcgccaggc cgaccggcgc acaaatcaga aacgaccaca
     2401 gcccgggctt ggcgatcgtc agaaggaacc aggcaatcag gaacgggggg aggttcaccg
     2461 gccgcactgg cgcccacaag cagcttgcca agtcgcgtat gaatgggccc cacacgcccg
     2521 gcgcggcctc ctgcttctcc tcggcctcgc cgcgaaggcg aacgcctttc tcggccttgg
     2581 cggtgcgctg cggtttatcg actcgcagac ggacggtgga gccttcgcct ggcggagtaa
     2641 tggccgtgag tgagagccgg ccgctatcgg ggggtggttc tggaacctgg aatggctggc
     2701 cgcacttctt gcacttggcg cggcggccgg cgacttcatc gggtaccttg tagcgggcgt
     2761 ggcaggccgg gcattgggcg atcagcatgt ggtctccctg agggcggttt cgggtacggc
     2821 gagccgcctt cgcaattgtc atgatggtgc cagcctggaa tttgtgcaat cggccagccg
     2881 gaaggcctgc cggcggcagg tcggattgga caggccgggc cgatataacg aagcgccgcg
     2941 aacggccggt ttccgggcgc atgccttgga taaagctgag agatttttcc ggccctgcct
     3001 ggaccgatgc tgggccccag tagcctcccc gccgggcgac cccggaaact cctcagattt
     3061 ctttgccctg gcccggcaac gtatcgtatg gtttggttgc ggaccgttta cccctccgca
     3121 cggagcatgc gcatgtttac tccacgccat attgctgccc tgcgacggtt tgggcaggag
     3181 gatgacgggc cgacggctac cgaatatgcg gtcctgctgg cactgatcgt gttagtgatc
     3241 cttacagcca tcgtgccgat cggcacgagc gtaaacgacg cgttccggtt catggtcagc
     3301 cagttgggcg gctaactacg cgcagcgcat gaaaaggccc agccccgtgg cggccgacgc
     3361 ttgcgtcggc cgaacggagc cgggcctgat cgcatttcca aagcggttta cgcccgcggg
     3421 tgcgcttcgt cgtaggtttt gcggacccgc tcgccactca ggtgcgtgta aatctgggtc
     3481 gtgctcaggc tctggtggcc gagcatttcc tgcacgcttc gcaggtccgc cccgcgccgc
     3541 agcatatgcg tggcgaaact gtgccgcagc gtgtgcgggc tgacggacag atccagtccc
     3601 gcctccagca gatatttgtc cagcttacgg cgaacgctgc gcgtgcttag ccgccggccg
     3661 tgcttgttga tgaacagcgc ctcggcgtcg aagttcccgg agcgcgaatc gttgcgccgc
     3721 atgtcgagga agtgcaggat ggcctgcacc gccccggggc cgagtggaat catgcgctgc
     3781 ttcttgccct tgccgcgcac gcggatcacg ctcgtgtcca gcagcacgtc ggatacatcc
     3841 aaagctacca gttcgctcac gcgcaggccg gtcgaataga gcgtctccag catggctcgg
     3901 tcgcgcgccc ccagcagcgt cgtggtgtcc ggattggaca agagtcgctc gatctgttcg
     3961 atctccaagc acttgggcaa acgcttgtcc tgcttgggag tgcggatggg cgccaccgga
     4021 ttgctggaca agtaggcccg ccgcaccagg aatttgtaga agctgcgcag cgtcgcgagt
     4081 ttgcgcgcca cggtgctctt gcagtaattc ttctcgcgca gctcggagag aaacccacgt
     4141 acaacgtcgg tgtccacttc cagcaaacgc cggttcatgg cctcgccgcc gatttctgcc
     4201 gtggccggaa gccgctccgc cgggccgttg ttgccgccgg tgctgacgtc gtgcggcgcg
     4261 cacttctgac cgaccaaata ctggcaaaac tgggccagat ccgccgtgta acacttggaa
     4321 gtgtgtggcg agaagtgccg ttcggcacgg aggtagttga tgaactgctg aatcagactt
     4381 gcttgttcgt ccatagtgct gccatcgctt ccggcgtgcg gtccccagtt ttgctgtact
     4441 acaacatcgt tctgcatggt gcttctctcg atctatcgtc acggacgcaa ccagtctccg
     4501 agtcaatcga ccggcccaat cggaggctga tcgccgcggc gtctagccag tcaaaggcgc
     4561 tatcggggtc gtttgcgagc tcaagcagcg cggtcacaag ctcggcttca cgcccctccg
     4621 cgtaacgaaa aacgaaacgc tcccggccct tgaccagtac tatcgatctg cgcaacctgt
     4681 tcatagccgc gcctcggctg cagaaaccgc ccattcagac ttttctacag ctaatatcga
     4741 tctgatcagg gaccagcaac agtagcgaac gtagagttcc tgcgatttta tgtaccgctg
     4801 ccagccgtac ggactcaagt gtccgtcgcg ccggcgggca aagctcttga cctcgtcacg
     4861 tacttcttcg tgagcggcat cgaacaccct ctggagctga gtctgcggcc ccgtcggaat
     4921 gaccgataac gccgccgcgg tttcgcatgc gttggaatcc gggcgtaacg ccgtttgtcc
     4981 gtcggccgac agcggcgcgt caggaacggc gtaccactgc atgatcaacc cgactaccgg
     5041 cgggtcgtat ggccgatact ccgtaactgc tgtcaccagc gtggcttgcg cgccaaaccg
     5101 tcgagccaat tccacagctt cagccgggct ctcaacacgc gttgtgcctc gttcggcaag
     5161 cgccgcgagc acgagattga ccggcaccac cccaatgcct gggaatccga ccgcctcaga
     5221 ggccacggcg tcggtcacct tcagcgtgtc aaactcggtt gagttgctga ggttcagaac
     5281 cggcgcgaca acaattacct gccgcggcgg aatgggcttg tgcggttgat gagtattgtt
     5341 ccccgggtgc gccgatgcgc agcccccact gacccccgtc gcagccacca gcgcaaagaa
     5401 tgccgtgccc atacgtattc tgggcttggt gagcagcatt ccgaccgact tccttgcccg
     5461 gaggctcgcg cgtcgcataa tcttacacga gttccaggcg tccggtaaga tgtctccatg
     5521 cgaatatgtg tgaaccagtc ccctccgagt gggactaatt tgaggcggtt tgctacaaca
     5581 cctgtctgga tttgagtcca gaacacacgt cggccgcagc atccgctcaa tagcgttcgt
     5641 cacgatcctg gcttcttttg ggtcaattta ctaccaaacg gctctcaaat actgcccgtt
     5701 cgcggccgaa cgagtcgaac ctgggtattt cgagaatcca agtactgtgc tcgccgccaa
     5761 cctggttatc ggcacacagc ctcggtcggt tgagtgttgg ggggggttga ccagaattcg
     5821 gggcgctgct tcgaggggcc ggcttgacgc agcgcttcca ggaccgtttc gccgctgggc
     5881 atttcaggca acacgatcgg ttcgttcggc ttgtacgccg gaatcattac gtaggtcggc
     5941 acgcctgacc tgccgtgctg ccgaagctcc tcctgtatct ccgaatcctt ggtcgtaaaa
     6001 tccacgcgaa ttgggtaaac gccgaacttc gcaaactggg ccgcgacctc ctggctgttg
     6061 agcacgacag cttcaatggt tttgcagttc gggcaccact cggccgtata gtccaagaaa
     6121 accgtgttgc cctccgctgc cagccgttgg gccattcccg gcttccattc ctgccaaggg
     6181 atccgctcca cgcgcggcga gaagacccag aagctcagga aaccgccgcc gcccgcgaat
     6241 agcagcgcca ggctccagtg agcagcccaa cggccggtgc cgtcgctcag gcggattctt
     6301 ccgatcagcc agcagccgaa accgatggcg caaatcaggc ccagcgttcc ctgcagcatg
     6361 gcatggtcga tcatcttcat cagtatcgag agcagccaga ctacgaccgc gatcagcagg
     6421 aaaccgacca cctgcttgaa cgtcaccatc caaggtcccg gtttcggcag aaatctgagc
     6481 cagctcggga aagccgtcag cagcagatac ggcgtagcca accccgcgcc gaccaccatg
     6541 attccggcgc cggccaggta cgggttgggc aactgcatca ggacgccgac tgcggtgccc
     6601 agcaacggcg cgacgcacga agtcgccaag agcgtcgtaa ggatgccgtt catgaatgcg
     6661 cccacgtagc caccgcgggc ggccgcctcg ccggcggcgc tggtaatcct gccgggcaag
     6721 cttatttcgt aaacgcccag cagactgagg gcgaacgcga acacgaccgc gaccaggccg
     6781 atcaggaact cggggcgctg catcaagccg ccccacgcca gaccggcata aaccatcaaa
     6841 atcgcgagca ggagaaagct ggccataatg ccggccgtgt aggccaggcc catgcggaag
     6901 atgcgcccgc gctgctcatg tgcctgctgc acgaagccaa aaattttgag cgagatcacc
     6961 ggcagcacgc agggcatcac gttcagaatg gccccgcctg cgaacgcggc caggaagacc
     7021 cacagcagat tcggcggaat cgcagtcgta cccgctcttg agggcgacgc cgcgggcgag
     7081 gcttcgccta cttcgcctag tttggcgagt atgggatccg tgctcgcaac cgccggcgcg
     7141 ccggcaggga cgacctcgaa ttcgatgtag ccttcggcca ggttgctggg aaagcagatg
     7201 ccggtgtccg cgcagacctg gtactcgaat agcgctcgta gccggagttt cttcggctcg
     7261 aagtcctgtt cgacttcaaa gggcgcctgg atcacgaaat cgccccccag ttcacgcact
     7321 tttccgagct ccgggtattc cttgacgtac ggctgcggcc aaacctggtc gaggaatttc
     7381 aggccgccga cgtgttcgat gtacaaccgc gatgggatca gcccctcgac tcctgggtcg
     7441 cggtcctgta tgtgatgtcc ggacttgacg cggacggcgg cgacaatcgt gccgcgttca
     7501 cccaccggca gttgtgtgtg cgcggccaac acccggcttc ccgcgatgtt gtccgcctct
     7561 gaaaactgct ggggcaggtt gcgccgggcg tcttcgaaaa gctgctgatt ggctggcgcc
     7621 ggcgggtcgg tcaaaaccgg caaaaccgcg gtcgcctcgg cttcggccgg gacgcacagc
     7681 cccttgcagg ccaaggcgct ggcggtggcc ttcagcgtca ggggtccggc cggagtatcg
     7741 ggcgcgacgc gcaccttcgt cagcatcact acggggcttt ccagcccaag aaactcctgt
     7801 ttcagctctc cctcagcgat ggttgaccat gttggggcgg gaaaccgcag ctcaccgacc
     7861 gtcacgccct ttggcagctc gaattcgact tccgtcggca ggccggtgtc gaggatcagc
     7921 gggtgataga ttttccagcc cggagcaaag cgcagctcga tcgccaggtc ggcctctccg
     7981 ccgggttgga ccgaagtctg cgcgaagctt aggcgggcct gaaaacccgg accggaatcc
     8041 ggtgcggctg cgggttcaaa cgcagtcgca gaaggcaggc ccgaggaaag cgcggcctcg
     8101 agtgccgagc ccggtgaagg cgcgaacggg gccacggggt tggcagaagg tgcggtttgg
     8161 aataacgcga tgagtggcgc gagcagaaca gctaccaaca ttcagacatc tctccgttac
     8221 cctcgtcgtg ttgggtgggg cctgaattcc cgccacctta tcttgaattc agctcgcggg
     8281 tgctcgagat tataagccca tcgcgggcca actgaacacc ctgtaagcag tacattatca
     8341 gaatcgtaag gttgcggcgg cgcgaacttt tttcgaggat ttcgcggagg gcaaagctcc
     8401 gctttgcctg aaaagcctct ttacgtgccg cgcaactcgg tcaggattcc cttacaagcc
     8461 gccgcgcacg gcggaaaatc gcgtcgaaca tctgctcggt caggcgcccg gtgaacgtgt
     8521 tctgctggct gggatggtac gaggccaaga gcacgcggcc ttctggcagc gcgaattcgg
     8581 ccccgtgacg aaacgggccc gggcgcacgg ccagacggcg cgccgcgttc acccacgcca
     8641 cgccgcccag cgccaccagg acacgccagg gtacgcggcg cagcgtttca tccagataat
     8701 cggaacagtt tcggatttct tcgggcagcg ggcggttttg cggcggtgcg cagcggatca
     8761 cggccgtgac aaacgcgttc tccagctcca ggccgtcatc tatgctcgtg gcggttgctt
     8821 ggttcgcgaa gccggcacgg tgcaaagccc ggaagagcca atcgccgctg cggtcgcccg
     8881 taaagagccg gccggtacga tttccaccgt gggcggccgg tgcaaggccc atgacgagca
     8941 gccgcgcggc cgggtcgccg aagttcggca cgggccggcc ccagtaggtc tgctcgcgga
     9001 aggcggcgcg gcgcagcgcg gcgatggttc gacagaattc gcgcaagcgc gggcagcgct
     9061 cgcaatcaat aactcgttgg ttctggcggc gaacccaaag cggccaggcc ggatcgagat
     9121 agtaagaacg aactggcttg ctctgggcag tcaaccggcg acttccttgg cctgctctaa
     9181 gcgctccaac gcaccggatt cgcggaggct caggctaagc aactgccacg ggtccagaag
     9241 aaacgccacg caacccgcgc cattctccac ccgcacgcac agtgcgaccg aagagcccgg
     9301 ccgcagaggt tcgcgggccg cggggacgct gattttcagc agcagaattg gaccgccggc
     9361 ttgggatgaa atggcggctt cgcagagctc agcgccgcgg aggcgcggtt gaccgccggc
     9421 gggccactcg agctgtgcgc tggcgtagac ctggtcgctg ccaacgaacc agccgtcgtt
     9481 gttgaagtcg atcggcgcga acactcgccg tacgggcttg cgcagtgtga aaaggagcgt
     9541 cagcgtttct ggtgtgtaaa cggcatatac gtcggtgtca caccaggcgg agctgatccg
     9601 gcctagcttt tcaagcaggg gctcgggcgg ttcgccggcc aggatgcgct gcatcaattg
     9661 ctcatctgcc cgcgcgatgg ccgtcttcac cgcgaactgt gggtagggtt catgcccgtc
     9721 gctcgttccg ttgccgttgg tatcgcgctt cagcggattg gtgctgctga acgcgccggc
     9781 cgccatttcc tgcaagtcgc tcagaccgtc caggtcggag tcgggctccc acgagtcgga
     9841 accgaagcga ctctcgtcca gcggcagcgt ggggtcgtcg tccgccaagc cgtcctcgtc
     9901 gcggtcgtcg accagccgcg gcgtgccgta acgcaaccgc agccagttca tcttgtcggc
     9961 gcggcggcac aggaaagcgt tgcagtccca gtgctcgccg taacggccga tgtgcagcgt
    10021 gccgtcggga tggtttgacc accagtcgtc aaaacccgac ttctggaaga agtcgtcgag
    10081 ctggtgtccc cattcgtggg ccatgagcca ggcctgaccg ttattccata ggccgtaact
    10141 gctataggcg taaatcgtca agccgccgcc gtccactgtg ccaatatcat cggtgaacgc
    10201 cgggtcgaag ccggacatag ggtggattgc gacgaggctg tgatcgggtc cgatgggcgg
    10261 caggccgcgc ttctgccgca cctggttaac cagcgtgcgg gcggccgtgg gttcgatcca
    10321 gccgtccgcg attcttggct cggcgtcatc gatcaacatg aattcgaatt gcttgttgag
    10381 cgtgccataa ctgttgcgcc agtagaagag ccgatcggtt tcgagttcgc gttgcaggcg
    10441 ctcgattacg ccggtgggtt cgattgtggt cagcgcgcca ttttcgtcga ttgccttcgc
    10501 aaaaaccgca acgagcaccg gcatatcgat gacgtatgtc ttgccgcgcg gcggcgccgg
    10561 ggggagcgaa tacgcgacca actcgtcgac cagcgcgggc ggccaactct cgcagaccgg
    10621 ctcgctttgg cgaacaagaa tgcggccgcc gtccttcggg gggcgctcgc ggctcactgt
    10681 cgtgaccgtg gaaccgtcca ccttcgtcat cgtgagcttc acaccgccat cgcgcgtcca
    10741 ttcgaactgc gggagcttct ccacaaagtc gggcgggaca atgaggattc gatctttctc
    10801 cggttcggcc aggtagtact tgccgtccgg cccctggcag acgcgcagcc gcgctgacag
    10861 gccccctagc gcggccccgg taaagacgcg ctctgccgcc ccgctgaaat tcatgacgcg
    10921 caacgccccg gccgccgtgt tggattcgac ggggcccgcg agcagtacgc gaccggcgtc
    10981 gtcgaaccgc acgtcaacgc cgggcaacca ggggccggtc gcggtccact tcatccgatt
    11041 agggcgcggc tcgtacttgc agaccgcgta tgtgcagttg ccgcggtcca tgaccaggac
    11101 gtcgccctga ggctcgacaa gaatcccaca cggctcattg aacttgcccg cgctgttgga
    11161 atccgcgtgt tcgtcaccgt cgaccgcgcc ccgcttgcca taagtcagca attcgccacc
    11221 ggccgcgtga aagacgcgga ttccgggaat gccggcgtcg gcgacaaaga tgtggccgcg
    11281 cgggccgagc gctaccgccc tgccgcgcat ctgcggcacg cgcaccagcg gcccatcacc
    11341 aagttggcgc agccggtggt cgaacaggta gagcgccgct ccatcctccg ccagcacgag
    11401 cacgccgggg tcgccgtcgc gtaacggcca gtcgaaccag gtgagcgcga ccggattcaa
    11461 gtgctcgccc agcaagccgc gtatgccagt cacattcgcc caagctacgt cgtagccgcg
    11521 cgggtcaatg agggtaagga actccggccc gcgcttcaga aggacgaggc agttttgcga
    11581 accgatcagg tccagaatgc cgctcgttac tgcggccggc tttgaggtca gcaatgattc
    11641 gtagggcgta tcagcgaaaa tgcgcagatc gttcacctgg ccggcgttgc gagaggccgg
    11701 cgtttgggcg ttcagaagtg gcgctgccga gaaacatagc caaaggcaaa gtgcttccgt
    11761 ccgcacggcg aacgctcctc tgcgcgccac ccctgcgcag aggatagtca aggggcggcc
    11821 gccagggaac tgccgggcgg gcgaggctcc gccgaacggc gtctcggcag gagcctcgtc
    11881 caccgccgcc ccgctatccg ggatgttggc cctcgctggt gtttcgggcc cggatcgcgg
    11941 agcggctcgc tggaggcacg tcgtcagtca accgccgccg ccaggctact tgccaggctt
    12001 gggcggttgg ccgccgggcc ggctcccggg ctgagtccct gggtgggttc ctgtcgggcc
    12061 gctggcagat gcagcggtca ccggctgcgc ctgcggggga gctgcagggc ggccagcgcc
    12121 tgctccatcg taggagctcc actgctgctc gtggccgccg gctgctgccg ggctggtttc
    12181 ctgatatctg cgcctggcca gctcctggtc gattttggac actcgctctt cggttagcgt
    12241 gctgccctgt attggctggc gtaattgccc ctcggtttcg tgcacctgcc tgatcggctc
    12301 ggggatgtac gcccattcgc cctggtgcca cacaccgcgg ccctggttcc agggtccgcg
    12361 tgcgtcctcg ccggccgaca tgttgtagta ggtatgcgtg aagcgcgggt caccctgcag
    12421 cacgcccggc gggaagttcg ggtcgatact tcccaacgcg gcggtgaaca tctggaagtg
    12481 cgcgatttcg cgggtcatca ggaagcgcaa agactcctgc accagcgggt cgtcagtgaa
    12541 ctgcaggagg tattcgtaga caatcttggc ccgtgactcg gccgcgatgt tcgaccgcag
    12601 atccaccgtc agatcgccgt tcgcgttcac gtaggtgccg ctccaggggg tgcccacgct
    12661 gtcagtgacc gtcggcccgc cggccgattc gagcaggaac tgcggattgg tggcggccag
    12721 gtggataagc tcctcgcggg gtccgtttcc gcgcatcacc ttgttgagtt cgcagcgctc
    12781 caccgcgttc ttgagctcgc catttacacc cgccagcagc atggtgatgg tcgcgccgac
    12841 gatctccaga tggctgaact cttcggtcgc aatgtccata aggaggtcgt atttgtcggg
    12901 atagctcttg cgcacgccga aagcctgcac aaagtactgc atggcggctt tcagctcccc
    12961 gttcggtccg ccgaattgct gcagcaacag cttggcaaag tgcggatccg gtttcgaaac
    13021 gcgcgcattg aattgcagct ctttcacgtg atgaaacata agattcggcc ctcgtcacgg
    13081 ccccttacgg cgcgggtttc gctcgcgcag cgcagggcac tcacaactcc gcagcccgca
    13141 acgaacggcg ctgctcgtga tgctagaagg ccgcccgggt cgtttccgat attacggcga
    13201 tttactctgg aatggatcgg ttgtaagcac tatccagagc caaagcccgg catgacaaga
    13261 actggagttc ggttcttaat gcttgacccc cctatacagc ctgtagactg gcggttaaat
    13321 gaaggaaggc agcgctgcac ataattcggc agacgctgcc taccttgaat cttggccgag
    13381 cattcccggc ctcgctgcta gaagaggccc aacaccctaa gcgccgcgac gaagtcggtg
    13441 acctcgaagg tgtggatggc gaattggtgc aggccggccc agcagccgct catgagtacg
    13501 ccgggaagcg taatcgctgt caaccacaag tacttacggt tcatgctcgc atctccaatt
    13561 ggatacagga ctaccgatga tgacatcggt aaacaatgcc agtaaacgaa taccagaccg
    13621 aacggggcgg aatactggcc actccgcaga ccgcgtcaag cggttcttat cgtcaacttt
    13681 tccgccttcc tcaaatgtct taagggcccc cgacgcaggt gaacacgcca attatgcgac
    13741 caagacagtt gtgttgtccg ctggccgcgt ttcacgaagt tcagagtaca ctcggctagt
    13801 tcaatgagcg ctgacgcatt gccgtcaccc gacaggctgg cggatagtcg tcatcggacg
    13861 gcagaggcgc aacgccgccg tgttcttggc cgcggtcatg aggcgctggg cgccacgcgc
    13921 cgaatcagcc cgctcaaccg ccgcacaacg aaactttcgg tcatcttcaa tgtaaatatg
    13981 ctacgatgcg gcgcaaggcc ggctgtttag gcgagcccgg cggacggacc gcacaggagc
    14041 gaattatgca cgttctcaga ccttcttggc gcgcggcggc gcgcctgttg tggctggttc
    14101 ctgtaatgtt ggctgcggct ccggcggcgc gcgccgaatc gcaagcggac ctggagctga
    14161 ctcggatcgc gctttttaac agcggcgtcg gctacttcga ggcgagcgcg caggttgacg
    14221 gcaacgccac cgccgagctg aaattcagaa ccgaccagat aaatgacatt ttaaagtcgc
    14281 tcgtggtgca ggatttcggc gatggtcggg tcggcgtggt gggttatgcc gcccgtgacc
    14341 cgattgaaaa gacgctgcgc agttttgggg tggacctgac cggcaagccg acgctgggcg
    14401 acctgttgaa ccagcttcgc ggcgaacagg tggaagtgac cggaccgcgg acgctcagag
    14461 gcagcatatt gggcgtggaa aaggagcaag tgctcagccc ggacggcaag actgtacagg
    14521 tgatcgagcg cctcacagta tcgaccgagg ccgggttgca gcaggtcaag ctggccgaag
    14581 tccagagcat acgcctgctg aacgagaaga ttgataatga attgcagaag gcgctcgcca
    14641 cgctggccac agcgcacgac gccgacaaga aaacggtcag cataagtttt gaaggggaag
    14701 gtcagcgcca ggtgcgcgcc gcctatttgc tggaagcgcc ggtatggaaa accagttacc
    14761 ggctggagct ggggcaggac aagaagccct tcctgcaggg ctgggccatt gttgaaaacg
    14821 ccactgaaaa cgactggaac aacgttcggc tgtcgctgat ttcggggcgg ccgatctcgt
    14881 tccgcatgga cttgtacact ccgctatatg taccgcggcc ggtggagcag atggagctgt
    14941 atgcgtcgtt gcggccgccg gagtacgagg ggcaaattgc tgatgcaacc ggaaggtggc
    15001 aagcatatgc ggatgcagag accgcgcctg ccgaggacgc ggcagcgctt gcacctgcgc
    15061 caggcgcgcc aaccgtcaga gcacgcagga gcaaggcgct ggagatttcg ggcgacggtt
    15121 atatggagtt gcagcaagag gtgccaatgg cgttagaggg ggccggggtt cagtcagtag
    15181 cgtccgccgc ggaggccggc gagctgttcg agtatacgat ccagacgccg gtgtctattc
    15241 agcgccagca ttcggccatg ctgccgatcg ttaacgaggc ggtttcggcc acgaaggtta
    15301 gcatctacaa cccggccacg cacgcgaagt atccgctgaa cgggctggag ctgacgaaca
    15361 gcacgaacct taacctcatg cagggcccgg tgacggtgta tgacgacaat gtttacgccg
    15421 gcgacgccaa gctgccggac atgaagccgg gcgagaagcg gctgatcgcg tacgcgttag
    15481 acctgggcac ggaagtgctg gtgcggcaga agccgacgcc ggaggaatta gtcagcctgc
    15541 gtattgccaa gggcgtactg tggctgcggc acaagtacgt ggaccagcgt gattacgtca
    15601 tcaagaacaa gctggaacgg ccgcgcgaga tcattctcga gcaggcttac agcgaggact
    15661 ggacgctggt gcagcctaag gaaccatacg agcgggcgcg cggcgtgctg cgcttccgca
    15721 cgaccgtacc ggccggcgag acggtcacgt atccggtgcg gctcgagcgg ttggcggacc
    15781 aatcgctggc gctgacggac ctggggttgg accacattcg gttctacctg aagacgaagg
    15841 ccgtttcgcc ggcgctgcaa aaagcgttgg aacgcgtggt cgccctacgg accgaattgg
    15901 acgaggcgtc gcgaaaccgg gccaatctgg agcgctcttt ggcggaggcg atccaagacg
    15961 agacgcgcat tcgcgagaac ctgaatacgc tacagaagga cgcggatgct tatcgtcgcc
    16021 agctcgagcg gttcgagaaa cttgagacgc gcatcgagca gcttcggacg caggtggccg
    16081 agttgcgcca gattgaggaa cagaggcgga aagagttgca gcagtatctg ctgtcgttga
    16141 acgtcgagta gcctcgccag tagcctcgaa ggtaaaggtg tgccctagcc ggccttggcc
    16201 atggcaccct cgccactggc gcttaacgac gaccgccgcc gcgtccgccg ccgcgcatgc
    16261 cgctcatgcg cccgccgccg ccaccgcgcg gggcgctgcg aaccgcgctg ccgcgcccgc
    16321 cgccgccacc gcgtggggcg ctacgaatcg cgctgccgcg ccctgggctg ccgcccggac
    16381 gagcgctgcg cattgcagcg ccggagcggc ccggccctcg cccgcgcggg gcactgcgga
    16441 gagcggcgcc ggagcgtccg gggccgccga tgcgcgggga gctgcgaagc gcagcgcccg
    16501 aacgcgcggc tccaccgcgc ggcatgctac gaatcgcgtc tgaacgagtg cgcggcacgg
    16561 cgccgccacg cggcgcactg cgaatcgccc cacccgttcc gccgcgtggg gttgccggcc
    16621 cgagccgcgt gccaaagtct cgagtcccgg gcgtggcgcc gcgttgcacg gcggcaccgc
    16681 ggtcgaactg ccgccgctct atcgtgcgct caccgaccag cccctgccga actcgccggt
    16741 ctgttgggcc gagcgttcct cgcccggtcg ggccgaagcg gtcgcctgcg aagcggcggt
    16801 caaacgcccc accgcgttca aaggttccgg tcgtcccgcg gcggtcaaag gttccggtcg
    16861 tcccgcggcg gtcaaaggtt ccggtgcgac cgaagcggtc acgcgtgacc gtgaaacggt
    16921 cacgcgtgaa gcggcgatcg aacgtcgggc cacgatcaaa cgtccgccca aaccggtcga
    16981 agctgccgcc acgaaaaccc gtgtagcccc gtaagccgtc gaacgggcct ctgccgtaga
    17041 agccgcggct gccatagaac cggtccccga agtaacgccg gccgccatag ccatagtagc
    17101 ggtcaaaggc gccacgccgg tagacgcgat cgccatagaa gcgcccgccg tagaagccgc
    17161 gatcgccgta aaagcgtccg taccggccgt agaagtacgg gtggcggtgg aagtacgggt
    17221 acctgaagta aggatacttg tagaaccggt gaccgtagcc gaagttgaag gcgaagtatg
    17281 gcgcgccgta gctgtagtag taagagtaag gccagtaata ggacagactc gggtagtaac
    17341 tgtacgccag cggccagacg gcggggtagc tgtagtagta aggaactagc gggctatagt
    17401 aagtcgtggc cggcgccgag taagcgtagt ccggggcgta accatagtcg taggccgggt
    17461 actgctcgta agctgggtac tgctcgtagg cggggtactg ctcgtaaccc gggtactgct
    17521 ggtaggggta ttgcccggcg tagccgggtt gctgttcaag cggctcggcg tactggggca
    17581 agattccttc ctgcatctgc ggagcttccg cgaccggcgg ctgcgtggtc agggccggct
    17641 cgccccatag gcgagcattc tcggcaaccg caaatgccag gcgggcgtcc ataggctgat
    17701 ccggaagcgg ctgcatgatg atgggcatta agcgcgcatc actgggagcg gcgccgctcg
    17761 ccgctcgcca catgttggcc cgctcctgcg acggaagcgc agccaagaac gtcgcatcgg
    17821 gcattgccag tgcggcttgt gagaacacct gctgatcgac ggcggagatg ccgtacgtct
    17881 gccaccattg gttcagaata gaagccaacg gctcgggagg cttgcggtcc tcggcgaagg
    17941 ccagaagaag ctcgttcggc ggcgcggcct ggtagtactg ggggtcggtg accacgacgt
    18001 aagggaactg cgggtactgt ttcaactgct cgcgggtgaa ttcagtcacc aggttctggt
    18061 attgctcaag ggcctgcgga ttttgagcca acatctgttg ccaggcctga ttgccctgta
    18121 gccgtgccgc ccggtagcgt gcctgcaggt tggccaggag ttcgtctgcc tgctgcggcg
    18181 cgctttgata aagctgccgc agacgggcca tctcatccgg gaaggcagcc gccagcgcaa
    18241 ctacctcggg aacctgctga atctgccgga tggcgtcctg caactcgccc gacaccggcg
    18301 gggtgatact ggccggattc atgacttttt gcgggtcctc ggccagttgc tgcaaaacgg
    18361 cgggattctc ggatatcttg accatggccc gcacgacgcc cggcgggtag gcgcgtactc
    18421 ccgccagaag ttgcgtctgc gagatggccg ttgtccgcgc ggcgtcgggt gacgtataac
    18481 cacttggtgg ttgcgttcca cttgttggtg gttgcgttcc acttggtggc tgggttgatg
    18541 gttggaaaac ggtttgagac agagagtaag gcgcggccaa caaaatggcc gctgctgcta
    18601 gcagcgcttt gggaagcatg agacgaactc ctagagccgg catgcgggat gttcgacttc
    18661 caatgtacac tgacgacacc ggagagcgga ttattcccgg attgcggagt attaattcag
    18721 cggccccgta ttcgtgagga ccaacccatg ccaaacgtca atacctggca tcgaatcgat
    18781 ctgcagtgcg gcgacattac gcgcgtgaag gctgacgcga ttgtcaacgc cgccaactcg
    18841 cgcttggccg gcggcggcgg cgtggacggc gccatccatc gtgctgccgg cccgggtctg
    18901 cacgaagctt gccgcaaact cggcggctgc gcaaccggcg atgccaaaat cacgccgggc
    18961 ttcaatctgc ccgcacggta cgtgatccac acggttgggc cggtgtaccg cgacgggcaa
    19021 agcggcgagc cggaactgct ggcttcgtgc tatcgccgct ctttggaagt ggcggttgag
    19081 aacggggtca agacgctcgc gtttcccgcc atcagcaccg gtgtctacgg ttatcctttt
    19141 gaaccggcga cgcgcatcgc agtgcagacg gtggcggatt tcttgtctcg ggagcccagc
    19201 atcgagcgcg tgaccttcgt gttcttcggc aagtcggact tcgaacaggc gcaacgtgtg
    19261 ctggcggact tgtcgcgggc ggccacttga atcaccgtgg gtgactcacc gtgggtgcca
    19321 tgcccaaagg cgggtgccat gcccaagggt gggtgccatg cccaagctct aagcgcgggc
    19381 atgccctcgt taacggcgct gtgtgtgggg cgattggcga aggcatgccc gcgccgccac
    19441 gcggcttggg catggcaccc agcatggcac ccttgcgcct atgctgcccg ccgctatgac
    19501 tgcactgcga ccggctccgg cgccgccggc ggctgaagtt gcgaacgtag gtgcagccgg
    19561 aatgactcgc cgatctttgc cagccgcgcc gacaacggcg gcgagaaacc aaagcgttcc
    19621 gcgtcgtgcg gcgcctggat tctggccatg cgtttccagc ccttgttcat gcccagcacg
    19681 aacagcgcgg tggtgagcga gcgtgtgcgc accagggtgc ggatggcgcg cttcagcacc
    19741 cagttctctt tggcggccag gtggcgcagt tcgtagatgc tttcgtccag ctcgcccgag
    19801 gtcattcgca acggctgata gacggtttcg gtgaacgtgt atcgttccca gtcagccgga
    19861 tagtcagtcg ccagcaagcg gccctccgca cggaagcgct cgtacatttt cgtgccgggc
    19921 aagggcgtca ggttggtgat ttgtatgatg tccacgccca tctttgtggc catcagagcc
    19981 gtgtccgcgg cggtttcggc cgtgtcttcg tcagagccga tgatgaagcc gccaaacacg
    20041 gaaatcccgg cgcggtgaaa accgttcacc aactcctggt agcgggcgag actattgcgg
    20101 ttcaggacct tgtgatagcg cttcaacgtc tgctcgttaa agctctcgaa cccgaccagc
    20161 atgccgcggc accccgcctt gtacgccagc gccaatccgt ccgggtcatc gcccatgttg
    20221 atcgtcgtct gactgaacca gagccgctta cgtccgtgtt tgatgatggc gcgcagcaat
    20281 tctttggccc actcggcgtg ctgccggccg acgccgaaaa agttgtcgtc caccacgaag
    20341 atgaattttt ccggggtccg gttccattcg tccaggatgt ccgcgatccg ccggcggcga
    20401 atgtgcgagc cgttgaactg cgtcaccgag caatactcgc accccaccgg gcagccacgc
    20461 gacgtttgca acgctgagac gtcgtagcgg ccattgaccg gccggagcgt ctggcgggcg
    20521 cgccccaggc cggtggcctc caagtccgcg aggcggccct gatagcgcga ctgaaggcgt
    20581 ccggcggccg cgtcgcgcac gatctgcggc cagatttcgt cgcactcgcc gatggcgacc
    20641 gaatcaaagt gcagcgcggc ctcatcggga caggcggagg catgcggtcc gcccaggatc
    20701 gtgggaatcc cctgcttgcg cgcggcggcg gcgatctcgt aagcgcgggt ggcgccgctg
    20761 gtgtacgagg tcagggccac caggtcatag ttgctccggg tcaggtgcgt gtcggtgaac
    20821 tgggggccga aaacctcgtc gatcagatgc acctcatggc catcgggcac cagctccgcc
    20881 aggacctgca atccgagttg tgggatgtac gagccgacgg tgtggtagcc ctgtgatcgc
    20941 cgagttatgg ggttaactaa tgctatgcgc actgtaagtc ctttactggg gggcggccgc
    21001 tttgagcgca ttgtaaggaa gctaaccggt ttcagccatc tgcgatcagc caaatattcg
    21061 cggccgtgag ttatcgcacc cggccggttt ttgcgctccg accggacaag tcgtgccgca
    21121 aactctccga tgaaaaagct gaggttgcat aatcgcttcg cacactgtgg gcgtgagggc
    21181 ggccatggcg cgagcgcacg ttgaaaccat actcgcccac gagagcgtgc cggcgagcct
    21241 taaagaggcg atcaagcgaa cgggggcttg ggccagcttt cgaccattat cgcaggcgct
    21301 acgcggcggc gtctccccac gggccgacgc aatagtcgtg atcgtgccgg acgacatgcg
    21361 ccccgtggag catggtctgc gcgtgctctt cgaccgcttg gccgactgcc cgcgcgccgt
    21421 cctggtgatg caacccggga gcggagtacc cgcccaactc gaccatccgg ccacggtgcc
    21481 ggtcaccttc ggctcggcct tgggggccga cgagctggcc agccggctgg caacaatggt
    21541 cgacatgcgc ccctcactcg attcgctcca tcgcagcatg ctggccaacc gccagcagga
    21601 agaaaacgcc accgcggcct acgagcgcca actacgcctg gcacgccagg tacaacaaga
    21661 gttcattccc gagaacctgc ccaacttcgg gccggttaag ttctcggcct tgttccgccc
    21721 tgcggaagtc gtctcgggcg acatgtatga cattcatcgc ttggacgaag aacacgtcgc
    21781 cattgccctc gcggatgcca cggggcatgg aataccggcc gccctactga cggtgttcgt
    21841 caagcgcgcc ctgcgcggca aagagattca caacggcagt taccgcctgc tgccgcccac
    21901 ggaagtgctg gagcggctga acgaggaatt gctcgaggcc aacctgtctg agtgccgctt
    21961 tgtagcggcc acgtatgcgg tgctgaacac gcggaccatg gtgcttgaac tggcgcgcgg
    22021 cggaacaccg tacccgatcc tgcgccgtcg cgacggccgg ctcgagttgt tgcggccggc
    22081 cggtagcgtt attggcgtat tgccgggtgc gcgttatccg tcgcagcagg tccaactggc
    22141 ccccggtgac tcactggtga tttgctcgga cggggtggag aacgtgacgg cgccgcagat
    22201 atcggcgacg gctctggccg aaacattctc ccgggccgcg gcggccgtga gagcgggaac
    22261 cgcgcgtgag cggcgccgcg ccgccgcctt ggttggggcc gccgcttgtg aggacagcgg
    22321 gtgcttcggc ggcgcaagct caacgaccgc cgtggcggaa atcgcggatg agcccgcggg
    22381 ttcgggcgac gacgaagtgc ccggtattga gcgtcccagc cgccgcgcat cgcgcatggc
    22441 gccggacgaa gccatgcttg cctccgcctg gtgcgcgacg ttgcgagacg aaggcccgca
    22501 ggcggcgctc gagcagctca ccgtacggta tgactcactg cgccgcatgg gttatccgct
    22561 cgacgatctg accgtgctca ccgttcaaat acagccctga gccggcgtcc agccggaccg
    22621 tcttcgagcg ggggggtagg cgatctaact aagagtctgc cgctgtaacc ggcggtgacc
    22681 gcatttcaga annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcgtcaat
    22801 cccggccgca ctggctgcga cctatgcctg gccgccgaac aaatgattct tcagacgcgc
    22861 aaacgcctga gcgccttttt caaccccagc gcggttgccg cgggcaagcc gaaggacccc
    22921 aagcgcctga tcttcacgct aaacggcacg atgagtttga acctgatcgt caacggtatg
    22981 gtggggccgg gcgatcacgt cgtgacgacg atggttgagc acaattcggt catccggccg
    23041 gtgaaccacc gggtgcgcga cgccggcgcg accgccacct acgtcgttcc cgacggcgag
    23101 gggtacatcg acccggagga tatccgcaaa gccatccggc ccaataccaa gttggtaatc
    23161 gtcaatcatg cttcaaacgt cacgggcgtc gtgcaggacg tgcaggcgat cggcaaagtt
    23221 tgccgcgaac tcggcgtgcc tttggcggtg gatacggctc agacagccgg tgtgctgccg
    23281 atcgacatga ccgagtgcaa catcagcttc ctggcattca ccggccacaa ggggctgctg
    23341 gcgccgaccg gcacaggcgg tatctgcgtc gccgatgacg cggaaatccg agggacgata
    23401 tatggcggca caggcgtgcg cagcgccgac ccctaccacc tcgaggagta cccgtatcga
    23461 ctcgaggccg gcacacagaa cctggccggc atcgcgggat tgtcggcggg cctggattgg
    23521 ctcgaagaac acgggatgga caaggtccgc cggcatgaga tggagctaat ggccgagttg
    23581 cagaacgggc tggcagagat tcccggcgtg aagatctggg gcacgaagcg gctcgaccgg
    23641 cgtgtggcca cgctgtcaat cacggtcgcc aattacgacc cgagcgacgt gggcacgatc
    23701 ctggacgccg aacacaccgt gctggtgcgc acgggactgc actgcgcgcc gctgattcac
    23761 gatcacatgg gctcgtcgcc gcgcggcacg gtacggtttt ccatcgggcc ttacaacacg
    23821 cgcgagcatg tggcggcggc catccgcgca gttgaacaac tagcgtccga ccgcccggct
    23881 acgacccagg tacagggctg caagctatag cgcgccagtt gcccctggcg ctcgtgggcg
    23941 gccggcgtgt cctcaagcga tttcttccgg gactgtgttg aggttttctc accggcctca
    24001 ttgaatctga gccgctttcg ctcatcggca catgggccgg gttgctcgcc gccgggatgt
    24061 gcacgcacta acattgtcgt tttcgaggag cacgcatgag cgagattgtt cgcacgcgct
    24121 tcgcgccgtc gccgaccggc tacctgcaca tcggcggggc gcggacggcc cttttcaatt
    24181 atctgctggc ccgccgcctg ggcggaaagt tcgtgctgcg catcgaggac accgaccaga
    24241 cgcgcaacat cgaggcggcc gaccagaagc tgatggaaga cctgcgctgg ctaggcctgc
    24301 aatgggatga ggggcccgag gtcggcggcg agttcgggcc ttatcaccag gggcaacgct
    24361 tggaactcta ccgcgagcgc gccaagtacc tgctcgagac cggccacgcc tactacgccc
    24421 tcgaaacgcg cgaagagttg gagcagatgc gggccaccgc gcgcgcccgc ggtgaaaagg
    24481 gctttcgcta tccccgcccg cagcgcttcc cgaccgaggc cgaggcggaa caggcccgcc
    24541 gcgcggggcg cccggtcgtc atacgcttca agatgcccga tcgcgacttc acggtgcctg
    24601 atcgcatcct gggcgacgtg cacatcgccg ccagcgagct gagcgacttc gtcatcatca
    24661 aaggcgatgg ttggcccacc taccacttcg cggtcgtcgt tgacgacgcc gccatgcaca
    24721 tcacgcacgt cctgcgcggc caggaacacc tcatgaacac gccgaaccac atggccctgc
    24781 aagaggcgtt cggctatccc gtcccgacct acgcccacct gcccatcatc ttcaacatga
    24841 acggcacgaa gatgagcaaa cgcgagaagg acaaggtggt acgcgacgcc gccaaagcgg
    24901 ccggactcga cgaacaaaaa gtgatggaac tggcgggcgt ggccgatccg cagctcgtgc
    24961 ggctgtggcg cgccggcgaa acgcagcttc ccggcgaagc cctagcccgg ctggcaaaga
    25021 agctcgcggt ccacgtgccc gaaatcgaaa ttcacgactt ccgcaagagc ggctatttac
    25081 ccgaggtgct ggtcaacttc atcgcgctgc tgggatggtc gcccggcgac agccgcgaaa
    25141 agttcacgct ggacgagctc tgccaggtgt tcacgctgga gcgcgtcggc aagaccaatg
    25201 cccgtttcga ccgcgacaaa ctgctcgcgt tcaacacgat ggccttcgag gccgccgaaa
    25261 tgccgcgcaa actggccgcc ttccgcgatt ggctcagcgt gaatgagccc ggccccatga
    25321 ccgggctgga tgacgccacg ctggtgcgtg tcctggaagc ctgccatggc ttccgcacgt
    25381 tcgaggatgt ggaccgcaaa gccggcgtgc tgttcgctgc cgacgaagcg tacgcttacg
    25441 atgacaaggc ggttcgcaag tggctgctca agaacgaggc cgcgggcttg aaagtcgtgc
    25501 aggacctgaa agcactgctg gccgccgcca cggattggtc gccggccgcg ttggacgacc
    25561 tgatccgccg ctatgccgag cagcacaacc tggagctcgg caaagtggcc cagccgttgc
    25621 gcgtcgcggt gacgggcacg accatcagcc cacagatttc cgaaacgctc ggactgctcg
    25681 gccgcagacg cacgctggcc cgcatcgagc gcgccgtgcg cgtggctgaa tcactggccg
    25741 cctgagcccg gtatacaatg ccgcgaagaa taggcgaaag gagcgcgaat gaccggagtt
    25801 acctctgtat gtgatatgcg gcgctatgcg accggccttc tatgcgcggc cggcctggcg
    25861 ctgctggcgg gttgcagtgg cacgctggcc gggcactggc ggatggttga ggttcagccg
    25921 aaccggcaag tcttctgcat cgatgacgtt acgttcgctc gtgacggcag ctacagcgcg
    25981 accaccacgc tcaagggcaa gacgctggag gaatccgggc ggtacaagtt caacggcttt
    26041 aagctcaccc tttggcccga cgccggtggt tgccgccagt atgacgccct gctgaaattc
    26101 aacgagctgg aaattctgga cgcgaagaag accccgaagc agatggtaat attgaaaaag
    26161 gattgagggc aggcaggcgc tcaagtcccg cccgtcgggt gcaacgtcgg gtgccatgcc
    26221 caagctgtaa gcgcgggcat gcctgtacaa tgcggtcact atggtgaatc aaggaccacc
    26281 catgaccaat ccaccaaccg gaaaccgcat caggcgtcac gtaatctact cggggcgcgt
    26341 ccagggcgtc tgctttcgcg ccatcaccca ggagatctcc gcccgttacg acgttaccgg
    26401 cttcgtccgc aacttgcggg atgggacggt tgaactggaa gccgaagggt cagccgccgc
    26461 cgtggacgcc ttcctggccg aaatcgcgcg gcacttccag aacaacatca cccacgcaca
    26521 agtctcggat gttccgccgc ggaacgacga agccgatttc cgcatccgtt attaacggca
    26581 tcccaaaggg ctaatctcgg cgcgctccgc cgcgaatgcc ctacacgcgc cagccctcac
    26641 ggtacgtctt gctgatgtac tgattcgctt ccggcagatt cggcacgcgc aaattggccg
    26701 catcccactc gatacgccgc ccggtccgca gcgccacgtt gcccaacagc acggcctccg
    26761 tgaaggggcc cgcgtacgcg aagttggatt tcggaaaatc gagcggcgcc tgccccatgg
    26821 cggccatcac ccattcctta acgtgtcccg gcgagcgctc gagcatctgc ggcggcttgc
    26881 cgatctgccg catcagtgtc tctggaatga tgcgcgggct atccagatag tcgcccgtaa
    26941 tcagcagtgc gcctttcgtg cccaggaata agttgcccgt atccggcaga cgccgctcca
    27001 gctccaacgc ctccggacac cgcggcttca atccgccgtc gtaccagtac gagatgaacg
    27061 ccggccggct cgccgtcgcg gggaagtacc agcgcagcat cgagcacgtg ggaaacgcct
    27121 cgtccgtaat gggcgtggcc gcgatcggct ccacccaggc cggcgaaccg gggtccaaca
    27181 ccgcgaaaat cccgtccatc gtatggcagg ccatgtcgcc cagcgccccg cacccaaaat
    27241 cccaccagcc gcgccagttg aacggatggt agggaccggc gttctgctcc ggcgccggcc
    27301 ctttgaacgg ccgcagcggc gccggtccga gccagcagtc ccagtcgagg ctttcgggca
    27361 ccggctcttc ttctttgggg cgagacatac cctggggcca gatggggcgg tcagtccacg
    27421 tgtgtgtttc gatgacatcc ccaatcgccc cgctatgtac ccattcgtaa accagccgcc
    27481 aaccctccag cgcgtggccc tggttgccca tctgcgtggc cagcttgtag cgccgcgccg
    27541 cctcagtcag ttggcgcgcc tcccaaaccg tgtgcgcgag cggcttctgc gtgtacacat
    27601 gcttgcccat gcgcatcgcc agcattgtgg ccgggtagtg atgatggtcc ggcgtgccga
    27661 tcatgaccgc gtcgatgttg tcccgctcga cttccagcat gcggcgatag tcctggtatg
    27721 gccgcgcatt ggggaacagc tcggctgccc gcgccatgcg ctgcgtatcc acgtcgcaat
    27781 agcaggggca ctcgacgcac tccagttcgg tcgtcttgtc gaggtggtag tggccgatgc
    27841 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcactcga cgcactccag
    27961 ttcggtcgtc ttgtcgaggt ggtagtggcc gatgccggcg gtgccgatat acgccacgcg
    28021 caacttgtcg agctttggcg cgtattcgcc gcggtgaaac gccggcagcg gcgcgggagc
    28081 gttgacgggc cgcgcgctca tcccctcaac ctgagccgcg cttgggccgg ccgcctctac
    28141 cggctgaatc ttctctcgcg gctgaccaca cccgatcagt ccgccgccga tgcctgcgcc
    28201 cgcgatggtc gcggcggtct taatgaacgt acggcgggtg gtcttgtttg ctggctgatt
    28261 catattctcc tcttctctct ccggcggcgc ttgtcagtcg ccgtttgccg ccggcagcag
    28321 cggcgcccgc ataagctctt ccatttcctg ggtatacagc cgccgcacgt gttccaccac
    28381 ttctgtcggc gcgaagcgct gcttcacgtc gtctttgcga tggctgaact cgaccgcgcc
    28441 ctcgttcagg ctcttcttcc ccacgatcac ccgcaatgga attccgatca gatcggcgtc
    28501 cttgaacttg aacccggccc gttcgtcgcg gtcatccagc aacacgtcca agccggccgc
    28561 ctcgagttga tcgtgaatct cgaccgcggt gcgcagcacc tgctcgtcac gcggatccag
    28621 cgccagaacc agcacgtgga acggcgcgat cgacatcggc cacactatcc cgttcgcatc
    28681 gtggtgcgcc tcgatcgccg ccgcgatgat gcggttcacg ccgatgccgt agcagcccat
    28741 gatcaacggc tgcggcctgc cttccttcgt caagaacgtg gcctgcatcg cgctgctgta
    28801 cttcgtgccg agcttgaata cgtgcccaat ctcgatagcc gtccgcagcc gcagcggata
    28861 cccgctttcc gtcggcgaga gatcgccttc gaccacgttg cgaatatcag ccagcacgac
    28921 cttgtcgccc gacagcggaa aatcgcggcc cggattgacg ttctttacgt gatagtcggt
    28981 cttgttggcg cccgtagccg cgttacgcat ggtcgccacg tcattatcaa caatcaacaa
    29041 gtcgaccttg tcgatcaacc cctgcggtcc ggcaaacccc accgccgccc cggtgacttc
    29101 ctcaatcact tgcggatcgg ccagctccac cttcatcccg gccgcccggt tcagcttgaa
    29161 ctcatttatg tcgtgattgc cgcgaaccag ggcgacgata cgcttcggac gcggcgcggg
    29221 cgcatccttt agagtatccg ccggtacact gacgtaaatc aacgtcttta tcatttcgcc
    29281 cggcgtcgtg cccatgaacg cgcatacgtc ttcgactcgt ccgtgcccgg gcgtatgcac
    29341 caattcgagc ggcttcattt cggccggaac cgccgctagc ggggcggccg tggcccgctc
    29401 gagattggcc gcataatcgc cgttctcgct gatcaccacc tggtcttcgc ccgcgtcggt
    29461 gagcaccatg aactcgtgcg acacgtcccc gccgatcgct ccactttcgg cctgcaccgc
    29521 cacatacggc aacccgcagc gtccgaagat gcggcaatag gtgcggtaca tgttgtcgta
    29581 ttcagcgtcc aggctgtcct tgtcggcgtg gaacgagtag gcgtccttca tcaggaattc
    29641 gcgcgtccgc aaaacgccgc tcttgggccg cgcctcgccg cgaaatttcg tctgaatctg
    29701 gtagaggttg atgggcagtt gcttgtagct caccaggtag gcccgcgcga tgtcggtgat
    29761 cacctcctcg tgcgtcggcc ccagcaccgt gttcggccgc cagtcgcccg gcggaccacc
    29821 cagccgcagc agcacgtcgc ccatggcctg tacgcggttg gtttcctccc aaagcgaaat
    29881 cgggtgcagc acgggcatgt gcagctcgat cgccccggcc cggttcatct cctcgcgcac
    29941 gatctcttca accttgcgca gggtgcgata accgagtggg agatacgtat aggtgcctgc
    30001 ggcgagctgc cggatcagcc cggcccgcaa catcaatacg tgtgaaggcg ccgtggcgtc
    30061 tttgggtgtt tccttggccg tcggaacgaa atatttgctc catcgcatct cgtatcgctc
    30121 ccataaacgc acggcgcggg ccggcgcggt taatccggca ttgtgctgcc cgcccgggct
    30181 tttttcaaac cggcgccaga tattggtaca atcggccaac ggttaccggc cggccaagct
    30241 gacggttacc ggccgccgcg ccgcctgatg agtaaaatgt gccgtgttgc cgcgcgccgc
    30301 gcgttgattt gggaacggcc gacggaatta gctcgcacct cagccatttg ccggccaaga
    30361 ctttcagccg ctggtcggcg cggcatagaa ttgccggatc gccgctggtc caaccggagg
    30421 accgcatgaa ccgcctcgca agatctgctc tcggcctgag ccttgtcggc ctgagccttt
    30481 tcggcgcggc cgggctgttc ggctgcgtgc ccggaacgct cgtcgactcc gcacaaaatc
    30541 aggccggcgc ggccatcccg gcgggctcct attgggtcgt tgaaacggac gccggcctgg
    30601 acctcttcca aatgccggcc taccgcggcg atgcgggcaa atggcagacc ctcatggggc
    30661 tgccgaattg gtacacacgg gacggcctgt actcgcagga cgcaaccgga aactggacgc
    30721 tcgaaattga cggcgcagaa acagacctgg acagccttct gcaagtctgg gacgccccgc
    30781 cgccgacaag ctagacgatc cggcgtcaca cgtcgtagcc gccggcggcg cgctccaaag
    30841 aaagctgcac aacgctcgtt ggcgcacctt ggcttggcat cgggggtgct gacgcagacg
    30901 gcgtttgcag tcataatccg agcttgagca ggcttttcgg atgaggagca tttcatgcaa
    30961 gcacaagcgc gcgatccact gagcgtttgg gtgaggttga tttccggatg cctggcggcg
    31021 gtgcttatcg agggcagcgc attcgcgcag gaagcgtcgc cgagcagcgc gccagtcagc
    31081 accagcagtg cgccgacggc cgtttcccag tcggcgtcgt attggctgcg cgtctcgggc
    31141 gatcaggtga atctgcgcag ccgggccgat cgcaacagcc tgccggtcgc ccgcctcgag
    31201 cgcgaccagg tgctccaagc ggtcggaatg gagtatggct ggcaccgcgt gtacgctccc
    31261 gagggcgtgt tttcgttcgt ttcggggcgt tacgtggatc agaccagcga aaccgagggg
    31321 atcgtctcga cctccagcgg cgccttgcgc gtgcgcgtcg gcagcgagct cagcgaagtc
    31381 gatccgatgc agaccgaggt ccagaagctg ctgccgcgcg gcgccaaggt gaacatcctg
    31441 ggacgccgcg gtgagtggtt gaaaatcgtg ccgccggacg gcgtgttctt ctacgtcgcg
    31501 gacgactgcg tcgaacggat aacgccggaa gtggcgaccg ctttgcgggc aaagaccgcg
    31561 cgagcgccgc gcgccgccgg tgctgtcgcg gccacccgcc cagccgaggg gcccgacatg
    31621 agcggtccgt gggggcaacg cctggcgctg gttgaggcgg ccattgaggc tgagggccgc
    31681 aaagacgcgc tcgaacaatc gtggacaaag tcgattgccg aactgcgccc catagccgag
    31741 cagcgcgccg atccggccgt ggcgcttctg gcacagcgct ggatcagccg gctcgaagag
    31801 cggattgccg atcaggcggc gctgcgcacc atgcggcaac tggcagacgc ggggcagcgt
    31861 aacgaggccc agctcgagcg ggagatggag caaatccgca aggtgcgcga gcgtgctgac
    31921 agcccgtcgg agctgatagc ccgtgggcag ttgcttgaga gcttcgcact gcggggaggt
    31981 gtgagcccgc cctaccggct gatagatccg ataacgcgtg cggtagtcgc atacctggaa
    32041 tttccgctcg acagcgacct taagccagcc gagtacctcg gcctgtacgt gggcgtggcg
    32101 ggcgagaaac gcacggatga ggcgtttggc gctgatgtga taaccgtcag ccggctcgag
    32161 gtgctcctgt cgcgcggacc aggcggaccg caaacggccc cggctcgcac aagcccctga
    32221 accggttata atgccgcctt ggtggaccaa ataagtattc gcgcaaggaa gaagccatga
    32281 ttaagacccg gacccgcggg cgtttgttga caggcttggc cgctctgaca ggcttggcgc
    32341 ttgccggttg ccctttgacg gatattcagc aagcggccat tcttgagagc acaatcaccg
    32401 gcgtcatagg cgcaatcatc cagatagccg ttcaagccgc ggcgggcgtc tagacctctt
    32461 gaggcggatt gcggcaatac gtcagtaacc gaaaccgatc gcatactctt agaggagttt
    32521 cgatggcgcg tgcgcgaact cttgcgccaa ttgtgttggt cggcattctc gtgctgggcg
    32581 gctgcaccaa gcgccgctcg gccaacatcg ttcaacatcc cgaatggccg taccaggact
    32641 accagcgcat cgcagtcctg ccgttcaagg cctgtgatcg caacgcggcc gaggccgccc
    32701 gtcaggccga gagtctgctt atcgacaatc tgaccgcgaa cggctccttc accgtgctgt
    32761 cgcgcggcga cctgaaagat attctgaccg agcaggatct gtcgcggctg gccgacgtgg
    32821 ccgatcccaa cacttccgtg ccggccggca tgctgcaggt ggcacaggcc gtcgtggtcg
    32881 gccacataac gacctttgat ctcgatcagc agaaaaaaga gcggcgccag ccgcgttacg
    32941 ccatggatcg caacgggcgc atcatccgcg accgaaacgg gcggccgcgc gtggtggggg
    33001 aagacgtgct ggtcgaatac cggcacgtgg ctcgtgtggc cggcagcgta cgggtgatcg
    33061 acgtggccac ggggcgggta ttgctgagct acacggttcc gccggtggag aaggacgatt
    33121 cccgttggaa cagcccgccg agcagcagcc cggagcaact gggtattgaa gtagcccagg
    33181 aaatcgcgac cgcgttctac cgcaagcttg ctccgcagac gatccgcgtc aagttgggaa
    33241 gcgattgcct ggtcgtggcg ggcgagtact acgagggcga gtacgagcag ctcaagaagg
    33301 tgccggacga tctcgacgag ctgttggtcg tcacgcgcgg gctgcccaaa tcttgcgacc
    33361 gtaactgctt ccggatggcg gtatcgccgc gcgaaggcgg cgaatacatc gccgagcacg
    33421 agttcatctg gtcgcccagt ttgggccagc gcggcgaggt gctgcgcgta ccggtcgcga
    33481 agctcaggaa caacggcggc tcacagttca ctgccaagct cttcgcggtc ggcaacgaca
    33541 cgccgatact cgcgcgcgac ttcgagctcg taccttcgaa agcgaagtag accgggagcg
    33601 cggcatgtcg tacctgcggg gcttgccagt ccgccacatg gccgggctgg tctgataagg
    33661 caccgaaagc ttcgccgagc cgggggccaa gccagctcac cggcccggcg gcaatttttg
    33721 accccgcggg gccgaacccg tagaatgccg ctcaatagct tcaacagtgt caacgtggcg
    33781 gtcacgcccc agccgagtgg aggaatggaa acgacgctta tcgaggcctg cgccgcacta
    33841 gaacgccatg gcatgcagcc gcggcttgag ggcgacccgt cgcgccacat catcggcgtc
    33901 gccaccttgg aggacgcggg cgagagcgaa atctcattcc tggcgaaccc gaaatacgag
    33961 aagctgctcg cgactacccg ggccgcggcc gtcgtcgtgc ggccggacgt cacggcccgc
    34021 aacggtctca atctgattcg cgtggacgac ccatacgccg cgattaccat tctcattatt
    34081 gagctgcacg gctatcgcaa gcactctccg ccgccccgcg acgcgggctc gccgaacatt
    34141 gccgccagtg cgaagatcgg ggcgaacgcc caaatacacc cgggcgtgac gatcgcggag
    34201 gacgtgaccg tcggggacaa cgcggtcatt taccccggct gctacattgg gccgcgctgc
    34261 cggatcggac acaacgtact gctgtatccg aacgtcgtgc tatatgagga cacggtgctg
    34321 ggtaaccggg tgacggtgca ttccaacacc gtgatcggta acgacggtct ggggtacgca
    34381 ccggtgggcg gcaagtggat caaaattccg cagatcggca cggtcgagat tggcgatgac
    34441 gtggaaatcg gatcgaattg ctcgattgat cgtgccacgc tgggttgtac gcggatcggg
    34501 tccggcacca agttcagtaa tttgatcgcc atcggacatg gcgcgcaaat tggcccggac
    34561 tgcctgtttg tggcccaggt ggggatagca ggatcggcta cggtcgggag g
//
LOCUS       DAOSYS010000008        33854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1058_length_33854_cov_5.589041, whole genome shotgun sequence.
ACCESSION   DAOSYS010000008 DAOSYS010000000
VERSION     DAOSYS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 33854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33854
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1058_length_33854_cov_5.589041"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLU99_01060"
     CDS             <1..266
                     /locus_tag="PLU99_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO15671.1"
                     /translation="SAALHEAGKGEFDLLISDLGLPDGSGVELLQNVRAGGNDLPAIA
                     LTGYGQDEDIRRTREAGFRTHLTKPIDFDQLQRAVTETAVAAG"
     gene            442..3186
                     /locus_tag="PLU99_01065"
     CDS             442..3186
                     /locus_tag="PLU99_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO15672.1"
                     /translation="MSGLRGGRKAATWLGPSWGYALALAATVAAFLLRWGLPGVLAKT
                     PYLAFYPAVVVSAGLGGLGPGIVATVVSAVCVDLFFDQSPGFDLGDPVSASRVLIFVL
                     GGVGTSLIAGVKWSIQERERRQARELEELVELTDLGQMFICDEQSRITRWSAGCAELY
                     GFTSEQAVGRVSYELLRAELPQPLAEIQAALYRDGRWEGEIIHHRSDGRPVTVAAVWA
                     LHRGEGRAAPAILKVNIDVSEQKRIQASLRENEQRLQASFDNAALGILEVDEQDRVVT
                     ANRRVCELLGYAREELIGLTVHELTAPEDRSLSDQLNAELHAGNRERLDYDKRYLRRD
                     GSRVWVHVTVSSIRDEQGRHLRSIGTVEDISQRKAAEEALRDSERKYRTVADHTYHWE
                     FWLAPDGRFLYNSPSCLDITGYEPEAFVADPDLLRRIIHPDDRPAYRDHRGEALEGCA
                     TDEVEFRIVRRDGAVRWVGHACQPVYDVDGKYLGIRGSNRDITEKKRVEQELAAAKLS
                     AERAKAAAEHANKAKDHFLAVLSHELRTPLTPVLAAASMLERSGEFDAETRESLDIIA
                     RNVKLQARLIDDLLDVSRITRGKLQLKKRPVELRGILRRAVEVCGPDLTAAHVQLDLD
                     FENASQVVEADPARLQQVFWNLLKNAIKFTPPGGRIGLRAWGEGKQVAVEVCDTGIGI
                     EPHSLSRIFEPFEQGEPGTTGKFGGLGLGLAISKALVQLHGGTITAHSPGQDQGATFT
                     VRLPIHTAQEATEPGEAAGPVGRAADQCQSGRLRVLLVEDHSDTARILCRLLTRAGHE
                     VALAGDIASAISEAAQRREFNLLISDLGLPDGSGLDLLRELRARGNPIPAIAITGYGQ
                     DEDLRRTREAGFVAHLTKPIELHQLEDAMALCQRYLTSPNCQVEQAGTPPRVCDAWPR
                     "
     gene            complement(3197..5368)
                     /locus_tag="PLU99_01070"
     CDS             complement(3197..5368)
                     /locus_tag="PLU99_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO15673.1"
                     /translation="MKDSGASLCRLSCIAVILAGGYWAAPSAAANEKYLIVSAPNYVD
                     SPALNLFIDHRTARGFDVSVYNVPSGTTRNEIKAYIQSLWGTPAAPAYLLIVGDTAGE
                     TSSSTTIPHWIGQGSRHSPTDLPYACMDGGDDWYPSMFFGRFSVTTTSALQHMVEKTI
                     AVESGGFSDPDYARRACMIATDDPTAEADGLHNYIIATYFEPAGFTATRVYGSVGGGT
                     ADIAAAVNDGVLFTVYFGHSTSGGWFSPAFYQSDVNSLTNNGLYGLAMGWSCNSAQYT
                     AAECFGETWLRAPNKGAAAYLSSSGLVWWGSSGAWESSRRMERYFFQSFFEEHICRVG
                     PAWQAALWKILADPDFGPAHDHTRNIFEEFVLLGDPALNLPFRALDMGLPEGVPEFIP
                     AFTPTTIIVRIDQGSEAYVEDSGTLHYRYSGGAFQTAPLIPQGRGLFAAVLPAPDCAA
                     VPEYYFSAMGDQGTVMKLPEKAPDAFFTSAVATVTTVLTEDFEVDHGWTVWNDPSLVT
                     GGWERCVPVTLGAVGTPGADFDGSGRCFVTDNRTPNFDVDGGPTVLTSPIIDLSGCSE
                     PYVRYAGWLYCDDYTPPAQDFLDVEVSSDGGETWIPAQHLAATGGWRVREIRVRDFVV
                     PSTQFRMRFSIADQPNNSMTEACLDALLIQNRSCAAAGDLNCDGAINAFDIDPFVLAL
                     TDVAVYATLFPACDYLLADINGDGAVNAFDIDPFVELLTAG"
     gene            5849..8065
                     /locus_tag="PLU99_01075"
     CDS             5849..8065
                     /locus_tag="PLU99_01075"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOO15674.1"
                     /translation="MHMPQQAQVEAQETRIPKFPENAYRELKPGEKFVPMIPPSMRVP
                     ELTPRMFIFGLLMNVIFSVAATFLALKAGQGIETAIPISILAVGLSGFLLKTGRRASS
                     ILENMYALSISTTSGYVAGGTCFTMPAIYILKLNSPESLNIGNLSLFLQISLVPFLGA
                     ILGALFLIPFRRYFVKEMHGKLPFPEATATNEILVTGASGSTTQAFILIYSFIISAIY
                     TFAATALRLFGDVFTTGLAHLKPVGKAPTEVLAFGSTGLFADLTEKVKAVVSMGTGAY
                     YLGLGFIIGLRYASIILAGSLLSVLVMTPLLGSLPLEQLQGINPDVKSTSAHDIWYNI
                     PRQMGIGTIFAAGLISILKMGGVIVTALRQAFGVLFSGKGGGTATDRTDEDISYGAMF
                     GIGLLVVIAMAIFFRTVVFAQMPNATWLTFVSVVLALVVTFLFTTVSAWAIAMISVTP
                     ISGMTVTTIIITAVLLAAFGLPRGEMAMLSVLLVGGVVCTGLSMAGGLITNFKMGYWL
                     GASPKRITWLALGGSLLSAVLVCGTIMVLSYQPGYTGDNALRAPQANMMAGALQSFLG
                     TGQPPWMLYGVGVVLALTMQLLGISPLAFGLGMYLDISINAPIFVGAVVATLVSKSTR
                     NEQLAKARTNKGILLASGLIAGGAITEVLVNFTTAIDELSLGVKTLVNGTEKMVGKIM
                     PFLDVSGRLVDAGIDPGDLARYQNWLGLILFLALCVFVYFDCRRAKPELGQGGHLG"
     gene            8148..10298
                     /locus_tag="PLU99_01080"
     CDS             8148..10298
                     /locus_tag="PLU99_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOO15675.1"
                     /translation="MACTPELQTKLPEIQTKLPENAYRELRPGETYVPMVPPNVIVPE
                     LTTRSIVFGIIMNVLWSVAATFVALKAGSGIETAIPISILSITLSGVLLRLGYRRTTL
                     LENINVLAIGSTSGIVAGGTCFTMPAIYVLKLNQNLGMGDMQLFLQIFLVPFLGAILG
                     VLFLIPFRRYFVKEMHGKLPFPEGTATNEILAAGAAGEGKQGLILVSSFVLAAVYNFL
                     CTGLKLFTGTFTTGMLNVSGEKVLAFPTTFFHTLVEKVKAVFFMGTGAYYLGLGFIIG
                     VRYAAIICAGSFLSWFVIVPLLGGFSIDTLHGLNDHPEFTDTTAEQIFKFVPRNIGIG
                     GIFAAGIISVLRMGKVIATALTQALGGLLKSKGSAGTPDRTDQDMSYSTILLLGLLTS
                     VALWFYFRFFVLADMPSPNRLTTISVLIALGVTFLFTTVSAWAIAMISTTPISGMTVT
                     TIIITAAALLAAGLQKGDGGMLATLLVGGVVCTALSMSGTLVTEFKLGYWLGASPRRI
                     QWSAIIASILASACVTATIMIFARSPGYVPSPEHPNALPAPQANMIATALNSFLGTSG
                     NVPWTLYGVGGVISIILWMLGISPLAFALGMYLPMEINTSILLGAIVAKLVERGARDE
                     RVLKARTDKGILVASGLIAGAAILEVVISLIGNFSWGEKLLAAIDINKRFIEAGADPA
                     QVMRTHNWIGLAAFLALCLFVYWNCRRAKPETVG"
     gene            10365..12653
                     /locus_tag="PLU99_01085"
     CDS             10365..12653
                     /locus_tag="PLU99_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HOO15676.1"
                     /translation="MAGRSEIQTQQSGSIEDNLKQAVASPAAAQPQVDQCKTGPAVGI
                     PTVASPAVLSPTADGQTVPRPSAPGLSARELSGRAIVFGMLANVLFSMIATYLALKTG
                     QIIATAIPISILAVGLSGFLLRLGLRRSATAENVNLLAIGSTASMVAAGTVFAMPAIY
                     LLGLQDSLDLGSPTIFLHIFLIPFLGATLGVVFMVPLRRYFLRQTRGEHSFPEAAATN
                     AILAAGQGGRTHTFVFVLSSLLAGAGAVCASMLRLWSETFTTGKLDITWVEKAAAGDK
                     AGQWVGRSFQSLALPGSPGDLWYSLTVKFKAFIAMGTGAEFLGLGYIIGLRYAAAMVA
                     GSMLSLLVVVPLLSQLDLGALQQLNPAINSTASDAIFNNIPRNMGIGCIFAAGAIWVL
                     KRSGGMAGSLGSALIGRFRQRGSSVVLERPGNDISYPVLGLMGIVAAAVMAGYCYFFA
                     FKGVPNGAALTVAAVVSALLISFVFSVVSGWATTRAGAPPISGVTVLAILIVPLVLLS
                     AGLSGSPAGQLGVLLVCGVAASAFSVAGSLMNEFKLGHGCGASPKKIAWSCIPACALA
                     CAVVAATIMLAARSGGFEGGASSTVLPAPHANLMKGAVLSILGDGEAPWLAYGVGAVI
                     ALAVQVAGISPLAFGLGMFLPMALTLPLLVGALIAAAVKWGTRDEATRQARHHKGLFI
                     ASGFVVGAALLGVINLALRGWAKAVNELNQLDMPSKLIGSGEDPEKVGQLMNWYGLLA
                     FALLAVFMLVIARWTRRPKNVA"
     gene            12884..16207
                     /locus_tag="PLU99_01090"
     CDS             12884..16207
                     /locus_tag="PLU99_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO15677.1"
                     /translation="MSRFASCATLVLIVAAFAVPALAESHLMRMADVHGDKIVFTYED
                     DLWLVPSAGGEARRITNDSGTEIWAKFSPDGKWIAFTGQYDGGADIYVMPANGGVPKR
                     LSFHPSNERMLEWWPDGKSVIFRSNFEYPFRGEKVYRVSIEGGLPEALPVDRAGLTAI
                     SPDGKQIAYNRITREHATWKRYQGGMAPDIWMGSLDKHDYRIICDCDFSSVFPMWQGD
                     AIYFASDREFGTLNLYKYETKDGTIKQLTKYKDYDVKYPSIGPDAIVYQYGESLHLLD
                     LKTGKTRMVPVEIPSDLTRMRPEFVDVRPSSGAFRLSPTGKRLLLEARGEIINFPVED
                     GEPVNLTKAPGSREKECAWSPDGRWIAFLSDRTGEEEVWLVDQKGENERQLTRGGLGF
                     RMHLEWSPDSKYLLFADKFLKLNLVDAASGELTVIGQADYDDAWERWGIQDYVWSPDS
                     QWVAYTKMEQSMNDAIFLYSLAEKKSYRLTNDQATSWSPSFDPQGRYLYFLSNRTFEP
                     IMCFQDQNHIFINMCKPYVVVLKADDTSPFYPKDSAEEVKGEPKPKDEAAGEDKPENA
                     EESKPAEKKKGKQGVEVRIDLDGLERRVLAADGVPAGNYFRLEATKDGFLYLAKDEPE
                     FEKYQVVTDRTGGKLDLYHYSLEKKEAKRLIKGIANYHQSADGKKLVYRDGSTYGVVD
                     VGKEAKVGDGKVDLGKIKIAVDRSAEFLQMFDEAWRIQRDWFYDSGMHGVDWPAMREK
                     YRKFVAFCGDRSDLNYLIGEMIAELNVGHTYVSGGDLERSGRRASTGLLGVEFAAEPG
                     AKYYKIARIIPGTPGDPQERSPLDEPGCPVKAGHYLIAIDGDEVHTNTSPFEFLQNKA
                     NVVVTLTYNDKPSAEGAQTWRVKTIASENRIRYREWVENNKAYVEKMTNGEVGYVHIP
                     DMGAGGLIEFARYWYPQYYKKGFIVDERYNGGGFTGDMIIDRLERRLWGLTQPREGKC
                     MRDPERCFHGPWAVVINEDTGSNGEFFAEAIKIKKLAPIIGMRTWGGAIGIEPHQDLV
                     DGGSVTPPQFGLYGLDCKWLIEGRGVDPDIEVQNMPGDVLAGKDAQLDAAIENVTRRY
                     RENPMDIPPPPPYPDKSKK"
     gene            complement(16311..17438)
                     /locus_tag="PLU99_01095"
     CDS             complement(16311..17438)
                     /locus_tag="PLU99_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15678.1"
                     /translation="MGAKKRLAVLLCLVLFVPAALSQGPRRPPREHDARDFDAPPPPP
                     SAGFWPTDRMMYLFIDRMTEELGKTYGFNEEQLHQTRELWKDEFPAWLNEHRHEIQQL
                     TNEYFEALLEDKPPDPEFVADWAQRVLPLVDEFAERCETVSEQMRPFLTEDQQLTLDG
                     QLAGFRVGMKYLNQRLVGWAEGGFDAETEWHRSPGFKENEEARNRQMAQEAQEAEIAA
                     RGGVPKSTGETGGAAGDAAGAARDNGRSAVSSRPAGDDWEKYVEDFIRRYQLNAEQQA
                     TAHKYLRTLQERREAFLRRNANELEKARQAAANPRSEAQRNRAEEQLQRLNRPLDNMF
                     TQLKEKLDSIPTRKQRAEAGSKAEAQDKTRDASAVGKAVKP"
     gene            17700..19160
                     /locus_tag="PLU99_01100"
     CDS             17700..19160
                     /locus_tag="PLU99_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO15679.1"
                     /translation="MGGRRLILAAALLLPVLPAGCQVDPGSLLPAEQGQDQSPEGLRL
                     VARFAHITDTHLNDTLSPARFAGAHALTRSAWRPYEAYSTQLMDGLIRAVNRIHASGT
                     RVDFLLHTGDAGEQAQSNELQWLVQIMDGGLINPLSGPDDRRADQRPPPELDPYAVFV
                     AEGLYRTGAHGDAPTIPWYTLFGNHDVYALGVFPILEVEPGRLLAPLPAPDRPGWLLP
                     TWLDPLSSFTHGRITPAHPGPPLLLECPSYLQPNPERAYFGKAHLIATMFGTLSPPAG
                     HGFAAADGPTWYSLSPAPGLRLIGLDTTDRTAQFPGQLCHDGAVSRAQLEFVRAELEA
                     AAARDELVVVAGHHPSQTIRADFGVQVTGEELREVLKQYANVVLYICGHVHRNRVIDR
                     GGYLEIETCSSLDLPQEGRIVEIWRDPAGPRIAISYRTFTHDGDDADDTLLPLRAAAR
                     AIALADKGAAARQKERDPSGVPPRGRPADREGWFIR"
     gene            19316..20683
                     /gene="thrC"
                     /locus_tag="PLU99_01105"
     CDS             19316..20683
                     /gene="thrC"
                     /locus_tag="PLU99_01105"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOO15680.1"
                     /translation="MHEQIQYYSTNRQVAPVSLAEALLQGQASDRGLFMPDRFPPLDL
                     AGLAKLRGRPYADVAYEVLRPYADGTFDDATLRALCDDAYDYEVPLERVTGRRYLMRL
                     DRGPTASFKDFAARMMARWMSVLMREQAGELIILTATSGDTGSAVAHAYHGVPRVRVV
                     VLFPPAEVSDRQRKQMTTIGGNVTTIGVDGKFDDCQALVKRAFADPALRDIRLTSANS
                     INIGRLLPQSVYYVYAYINLADVAAREPIVFSVPSGNFGDMMGAVIARRMGLPISRLV
                     IATNANDEVPLFLSSGRYRKISPSRVCISNAMNVGHPSNLARLVDVYGGMMDETGLLR
                     EQPDMEQMRRDLYAISISDDETRMMIQAAYERHNTLLEPHGAVGWAGLEAYVSENASA
                     AEALAVSVETAHPAKFPEEIRSLIGLDPPVPPSLAGLETRPEQYERMSTSYEPFRDWL
                     LGGFT"
     gene            20739..21911
                     /locus_tag="PLU99_01110"
     CDS             20739..21911
                     /locus_tag="PLU99_01110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO15681.1"
                     /translation="MSQLSAAYWPSIAPAETSAAPLVCSAVLTHHWLVRLRGGEKVLQ
                     ALCELLPGAPIYTLIHDPQEICRGWPPVRASILNRLPGVRRYYPKLLALLALAARSIR
                     LPEVDLVVCSDAAVAKAMRPHPRSRVVCYCHSPMRYAWDEQLRRQYCRTLPLTHVTPF
                     LLSTALARRIDAAGARRVDQFVANSRHVAARIRRDYGRESVIVYPPVDLPAEPSREAR
                     EDFYLCVGHHVPYKRLDLAVEACTRLGRRLVVIGEGPEVKRLRGQRLRGQGDVTWLGW
                     QPSEVIAECYRRARGLLFPGEEDFGIVPVEAMAHGCPVVAYGVGGATETVSDGVTGVL
                     FKEQHADALIEAMRKVESTVFDPVAMHAAMQRFSRRRFLGEMGAVLRAVCSDKVIR"
     gene            complement(21992..23086)
                     /locus_tag="PLU99_01115"
     CDS             complement(21992..23086)
                     /locus_tag="PLU99_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15682.1"
                     /translation="MQTSDASPPHSECKTPEFEAAPDRERRSRGYRWLIHLGLPASIS
                     LALHILLFGLLALKTWQVVASHAAEFSEYAAGVIQAPDQNTLPALDWTPPTPLAELEK
                     PTAIPDLGSLDFSRVTPLEPGALGTGPNLGPASDAMGLGDLGTGSLSLLGTGGGVAGG
                     AGSGGFGSSFGQRGDLGQAGVWNVSVPANRIVYVIDYSGSVIVAVDELKRELKHSIGR
                     LRPTQLFEVILFYGEGAGGQELSKADAFGSQLQPATQETRRKFFQWLDSKSPRGATDP
                     LPALKRALALKPQAIFFFSDGFFDDSVSDELTRANKSVGAKLVCLVFDEILLQDTSRL
                     PRETEGARRLRRIAEQNGGQCKIVTANDLP"
     gene            complement(23255..23644)
                     /locus_tag="PLU99_01120"
     CDS             complement(23255..23644)
                     /locus_tag="PLU99_01120"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOO15683.1"
                     /translation="MPDEQKKIIVDDDWKAEARREKERLAEQQQAAAHELMPEPSFAE
                     LVNLIAIQAMVALGLVGGPGGERIPPNLEAAKHFVDMLQVLEEKTKNNLTPEEKRALD
                     ATLYEMRMRYVQMAGGPIGPAPSAPGA"
     gene            complement(23864..25273)
                     /locus_tag="PLU99_01125"
     CDS             complement(23864..25273)
                     /locus_tag="PLU99_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO15684.1"
                     /translation="MTANKLILFAALVSSALACGAFADDWKTSGKNPQRNGQSADFGP
                     DSPTLAWSGGRYSMNAWPAFIEGQRLFTIRQQTWPGESHNDAFLVAMNIHTGAELWVR
                     AVPPYPGGWVPWVAGVNDGKVYVSRGGASFAPLYAYSAETGQILWTSEEDIDARPYDG
                     VVFAPNGDLIIGSFTDLWRISGEDGATVWHSSRICSAAGNCGAALYGDAVYIADLASG
                     GHAIVRYDLATGALQYTGDTMPGLVMQNTPFCGPDGTIYLSRTQNNPSYDYFYAFTDT
                     GSAITQKWSVPAAWTVASEFGIGPDGSIYMLKPGYEFVRLNPADGSVINSAGYIPSPD
                     IFIARMAIDAAGRVYLGNGSYEDGRLYAFDADLTLLWQTSVTDIYIGGPALGAGGTLI
                     VCGVDTDIRAYRTARGDMNCDGKMNTFDIDPFVLALTDAEAYLEMYSSCNIMYADMNA
                     DGAVNAFDIDPFVDALTSD"
     gene            complement(25746..26894)
                     /locus_tag="PLU99_01130"
     CDS             complement(25746..26894)
                     /locus_tag="PLU99_01130"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15685.1"
                     /translation="MVAFGVQTLRELVQGNSEPCVSFYVPLGAGPETEESRIRVKNLL
                     RQAEEALLERGFRRPEADKLLKAARPVLETARDSHPRDAALVALIAPNQLYEFQLPYK
                     CDPLSVVGERFHIKPLIPGVNQDRRFYVLALAQNDVRLYEGDRLELRPVHVPNLPANI
                     DEAVSPTEVERSVQWHTRTPVQSGRRGPIFFGQSAVGSDQIARLQHFCSRVDAAVCQF
                     LGDRQEPLILAAADSLQPTYRSVSRYAGLMPQGIEGNPEPTPLQQLHAQAWAIAEPAF
                     RAAELKHLELLPEAISKNRGSTDLAEILSAAMHARVAELSIAIDRHQWGRFDPETGEL
                     HVDPKQSPQNEDLLNLAAIHTLKHGGTVHAVSSTDLPNGSPAAAIFRF"
     gene            27103..27924
                     /gene="scpB"
                     /locus_tag="PLU99_01135"
     CDS             27103..27924
                     /gene="scpB"
                     /locus_tag="PLU99_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOO15686.1"
                     /translation="MDDLLVLDRVESTASDCTGFTVLTEAENASDDADFVPDALTADS
                     AQEPEASAATADAEPPQPEAAEELSEPRTADAPPTDQIIESLLFSADAPLSLNRLSEL
                     AGCSVMEARLAIADLNARYTVAKLTFRIEEIARGYQMMTLPRFQPWVSKLMAQRAQTR
                     LSPAALETLSIIAYKQPIIRADIEAIRGVAVGEVLNRLREMGLVRIVGRAEVVGRPLL
                     YGTTKKFLDVFGLADLEDLPPLEALNLRRRKPAEDGGAADEQSAAPPAERLAASA"
     gene            28052..28828
                     /locus_tag="PLU99_01140"
     CDS             28052..28828
                     /locus_tag="PLU99_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOO15687.1"
                     /translation="MPEVTKPSIEESGASRAASVTRARALALRVDELGRNRPDLVLIL
                     PYMVYLLLLGLVGLVPAKWEWAAIVVRGVGSLAVVYAFRRHLPPLGCPYWLIAVAGGV
                     LAAWLWYAGQYVFDAWGLGGRLPLYPGRKEVLDPRDLLGAGRLFWTTAVLRVAVAVTA
                     VPVVEELFWRGFMLRALLNWNDFERVPVGTFGWTAFIGTALLSTLQHPDNWGVSIACW
                     LFYNALFYWKRSLACLMLVHGLTNLVLYFHVLRVGDWAFW"
     gene            28989..30209
                     /locus_tag="PLU99_01145"
     CDS             28989..30209
                     /locus_tag="PLU99_01145"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOO15688.1"
                     /translation="MKRVLLGAVLVGLLVPLAARADLEEGAFAPDVDAVDWLNTDGKS
                     LSISELRGMVVVLYFWESWQPQQRYLLRSSNFAENQLRQAGVFLIGVTSAGRRTVEDL
                     IRQEHIFFPIALGSRAAEAYKIEPEDMPRVVIIDPSGAVVFSGPPNVGTVTQKVQKLV
                     FEEAPPFRTHPRHAEKARKALEAAREALKREDYQEAFVKAREAEDLALADDRLKLRCQ
                     EMIDLVDAIGRDRLHQGLALIETLQYEEGVKVINEVIKQFQVAGCGKAARRRLRLLKD
                     QYPQVRQVADKLGREDEAHTKLVSAAEKLWRRKFGEAYSALQKIEEEYSGTKAAETAK
                     VIRDRIDANKTLRQIVLDNDARKVCEDRLARARNFIETRRYEDARKTLRSIIDEFPQT
                     SYVEEAYRLLSEIP"
     gene            30264..31211
                     /locus_tag="PLU99_01150"
     CDS             30264..31211
                     /locus_tag="PLU99_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOO15689.1"
                     /translation="MNNKDPYQILGVARNASPEEIKRAYRRLAKEYHPDRNPQNKEAA
                     ERRFKEVQAAYEVLGDPERRAQYDRFGAGGPRPEFHNWGRGRPAEQPFGVEFDFGDLG
                     DLTSIFEQFFQRGPGRAGERRGARRTRVRGAELPRGADIEYPVEISFDESLHGTKREV
                     VLQSPEGNERLEVRIPAGVNDGQRIRVPGRGQPGAGGRGDLLIRVTIRPHPYYRREGL
                     DVLMDLPVTLTEAALGARVELPTPEGRTIVSVPPGTASGTKLRLRGRGAHDARRGTRG
                     DLLAAVRIVPPKTLTPRARELLESLSREVPQNPREKWGG"
     gene            31221..31646
                     /locus_tag="PLU99_01155"
     CDS             31221..31646
                     /locus_tag="PLU99_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15690.1"
                     /translation="MPQARRSLSGRMAVLLIVYALLLLVCVALYFFITNRLAASVARM
                     AAGEVGREVVIAELSRLVAIMLGCFLAILAFAIGAYTFIGIGRAISRPTASGKPTKYV
                     DAWSQYRISEEQIRAATEEVDDRNATEDPSSGRDDDDPS"
     gene            complement(31643..33052)
                     /locus_tag="PLU99_01160"
     CDS             complement(31643..33052)
                     /locus_tag="PLU99_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOO15691.1"
                     /translation="MTEPDRFSPTTLPEPGAQAKNRRRWPALHWQILIGLIVGAVAGL
                     VANRLGHGPDGLASPHLTWAIEYVAEPLGQIFLRLIFMVVLPLVFSALVLGVAGIGDV
                     RRLGRVGLMTLVFTVGLSAISVIIGVSLANALRPGERISAEQRAALSAQYEEQAGREL
                     AQARQAKPLRDALLDLIPRNLLREMVGAVDGSSPGGGMLAVMFFALIFGIALTLTPER
                     TKPLIAVLEGIYDVVMVIVGLAMRLAPLAVAGLVFALSARLGADILKMLAWYMATVLA
                     GLALHFFGVYSLMLALVARINPLRFYGRVSDAILTAFGTSSSNATLPVSLRVTEKNLG
                     VRREIGNFVLTVGSTANQNGTALYEGVTVLFLAQVFGVDLTLADQIKVAIMCVLAGIG
                     TAGVPGGSLPLVVVVLQSVGVPAESIGIILGVDRLLDMCRTTLNVTGDIAVAACVDRS
                     DAAGRTRRAAQGVRTDCGG"
     gene            complement(33159..>33854)
                     /locus_tag="PLU99_01165"
     CDS             complement(33159..>33854)
                     /locus_tag="PLU99_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15692.1"
                     /translation="QPGRNAQPVQPAEPAQPANSPAPAASAPAQTPPLLSPAPQLRIK
                     KIEPVPGATTQPATSMPAAASQPTILAPENITVKTPTPAEQAFERLKSLAGTWEMENA
                     PLAVPEGIKEFKTVFRVTAGGKTVLQTDFPDTVREYVNMYHMDGDSTLVVTHYCPLNH
                     QTRMRATNFDDPQRLSFVYHDSTNLDVAKNRHCHNTSITFISDDRIQQDWAFWVNGEQ
                     YRVRSATWKRVKN"
BASE COUNT         5956 a        10047 c        11435 g         6416 t
ORIGIN      
        1 tgtcggcggc gctgcacgag gccggcaaag gggagtttga cttgctgatc agcgatctgg
       61 gtctgcccga cggcagcggc gtcgaactgc tgcaaaatgt acgagccggc ggcaatgacc
      121 tgccggcaat cgcgctgacc ggctatggcc aggacgagga cattcggcgt acgcgtgagg
      181 ccggtttccg cacccacctg accaagccga tcgatttcga tcaactccaa cgcgcggtca
      241 ccgaaacggc cgttgcggcc gggtaggtct ggcgggtctg cggaattaat gcgtgacgca
      301 gattacgcgc ctatcatctg gaccgagatg cgagtttttt ccatgaggtg aggagttgcc
      361 tccgagcagg cgtttgggca ggctggtggg cgtcgtcctt tcgagccatt gtttgtttgt
      421 aggttgagcc gtggcgcgcg tatgtcaggt ttgagaggtg gtcgaaaggc ggcaacatgg
      481 ctcggccctt cgtggggcta tgccctggcc ttggccgcca ccgtggccgc cttcctgcta
      541 cggtgggggc tgccgggtgt gctggccaaa accccgtact tggcgttcta cccggccgtc
      601 gtggtttcgg cggggcttgg cgggttaggt ccggggattg tcgccacggt cgtgtcggct
      661 gtatgcgttg atctgttttt cgatcagtcg ccggggttcg acctggggga ccccgtctcg
      721 gcgagtcgag tgctcatttt cgtgctgggg ggcgtcggaa caagcctcat cgcgggggtg
      781 aaatggtcga ttcaggaacg tgagcggcgg caggcgcggg aactggagga gctggttgag
      841 ctcaccgatc tcggccaaat gttcatttgt gacgagcaaa gtcgcatcac tcgctggagc
      901 gcggggtgcg ctgaacttta cgggtttaca tcggagcagg cggttgggcg cgtcagttac
      961 gaattgctcc gggccgagct tccccagcct ctggcagaaa tccaagctgc attgtaccgc
     1021 gacggccgct gggagggtga gataatccac catcgcagcg acgggcgccc ggtaacggtg
     1081 gccgccgtct gggcgctgca ccgcggcgag gggcgggcgg caccggcgat tctgaaggtg
     1141 aacatcgacg tctccgaaca gaagcgcatc caggcgagtc tgcgtgagaa tgagcagcgc
     1201 ctgcaggcga gcttcgacaa cgcggccttg ggcattctgg aggtggatga gcaggatcgc
     1261 gtcgtcacgg cgaatcggcg cgtgtgcgaa ctgctgggct acgcccgcga ggagttgatc
     1321 ggcctgactg tgcacgagtt gaccgcgccg gaggatcgtt cgctcagcga ccaactgaac
     1381 gcggagctgc acgccgggaa ccgcgagcgc cttgattatg acaagcgtta cctgcgacgc
     1441 gacggctcgc gtgtttgggt acatgtgacg gtttcatcca tccgcgacga gcagggacgg
     1501 cacctgcgct ccatcggcac ggtcgaggac atcagccagc gtaaagccgc ggaagaggcc
     1561 ttgcgcgact ctgaacggaa ataccgcacg gtcgccgacc acacctacca ctgggagttc
     1621 tggctcgcgc cggacggacg attcctatac aactcgccat cgtgcctgga cattacgggt
     1681 tacgagcctg aggcgttcgt ggccgacccg gacttgttgc gccggatcat tcatccggac
     1741 gatcgacccg cctatcgcga tcaccgcggc gaggcgctcg aaggctgcgc cacagatgaa
     1801 gtcgaattcc ggattgttcg ccgcgacgga gcggtgcgct gggtcggtca cgcctgtcag
     1861 ccggtgtacg acgtggacgg caaatatctg ggcatccgcg gcagcaatcg cgatatcact
     1921 gagaagaaac gggtcgagca ggagttggct gcggccaagc tcagcgccga gcgcgccaag
     1981 gccgcggcgg aacacgccaa caaggccaag gaccacttcc tggcggtcct gtcgcacgag
     2041 ctgcgcacgc cgctgacacc ggtgctggcc gcggcgtcca tgctcgagcg gagcggcgag
     2101 ttcgacgcgg aaacgcggga atctctggac atcattgccc gcaacgtcaa gctccaggcc
     2161 cgcctgatcg acgacctcct cgatgtatcg cgcattacgc gcggcaagtt gcagcttaag
     2221 aagcggccgg tcgaactccg cggcattctg cggcgcgcgg tcgaggtttg cgggcccgac
     2281 ctgacggccg cgcacgtgca actggacttg gattttgaga atgcctctca ggtggtcgag
     2341 gccgatcctg cccggctgca gcaagtgttc tggaatctgc tcaagaatgc gatcaagttt
     2401 acgccgcccg gcggccggat tgggctgcgc gcgtggggcg agggcaagca ggtagccgtc
     2461 gaagtctgcg acacgggtat cggcatcgag ccgcattcgt tgagccgtat tttcgagcct
     2521 ttcgaacagg gcgaaccggg cacgaccggc aaattcggcg ggctgggatt agggctggcg
     2581 atcagcaaag cactcgtcca actgcacggt ggaaccatta cggcccacag ccccgggcag
     2641 gatcagggag cgactttcac ggtgcgcttg ccgatccata cggcccagga ggcaaccgag
     2701 ccgggcgaag ccgcggggcc tgtggggcgg gctgccgatc aatgccagag cgggcggctg
     2761 cgtgtattgc tggtcgaaga ccactcggac acggcgcgca ttctgtgccg gctgctgacg
     2821 cgcgcggggc acgaagtggc cctggccggc gacattgcca gcgcgatttc ggaagctgcg
     2881 cagcgccgcg agttcaacct gttgatcagc gacctcgggc tgccggacgg cagcggcctg
     2941 gacctgctgc gcgagctgcg cgcccgcggc aaccccattc cggccatcgc cataaccggc
     3001 tacggtcagg acgaggattt gcggcgcacg cgcgaggcgg gctttgtggc gcacctgacg
     3061 aagcccatcg agctgcacca gctagaggac gccatggctt tgtgccagcg ctacctcact
     3121 tcgcctaact gccaagttga gcaggccggg acgccgccgc gagtatgcga cgcgtggccc
     3181 cggtgagttg ccgtcattag ccggcggtca gcaactccac gaacggatct atatcgaacg
     3241 cgtttaccgc cccgtcgccg ttaatgtcgg ccagcaggta atcgcaagcg ggaaacagcg
     3301 tcgcataaac ggcaacatcc gtcagcgcca gcacgaacgg atctatatcg aaggcgttga
     3361 tggcgccgtc gcagttcaag tcgccggccg ctgcgcagct gcggttttgg atcaacaatg
     3421 cgtccaggca ggcctcggtc atcgagttgt tcggctgatc cgcgatcgag aagcgcatcc
     3481 tgaactgcgt gctgggtacg acgaaatcgc gcaccctgat ctctcttacg cgccagccgc
     3541 cagtggccgc cagatgttga gcggggatcc acgtttcgcc gccgtcggaa gagacttcca
     3601 catccaggaa gtcctgggcg ggcggcgtgt aatcatcgca gtacagccag ccggcatagc
     3661 gcacgtaggg ctccgagcaa ccgctcaggt cgatgatcgg tgaggtcagg acggtggggc
     3721 cgccgtccac gtcgaagttg ggcgtgcggt tgtcggtgac gaaacacctg ccggagccgt
     3781 cgaaatcggc gcccggtgtg ccaaccgctc cgagagtgac gggaacgcag cgctcccagc
     3841 cgccggtgac gaggctgggg tcgttccaca ccgtccagcc gtggtcgacc tcgaagtctt
     3901 cggtcagcac ggttgtgacc gtcgccacag ccgaagtgaa gaacgcatcc ggcgccttct
     3961 caggcagttt cattacggtg ccttggtcgc ccatggcgct gaagtagtac tcaggaaccg
     4021 cggcgcaatc tggggcgggc aacaccgccg cgaatagccc gcggccttgc gggatcagcg
     4081 gggcggtctg gaaagcgccg cccgagtagc ggtaatgcaa ggtacccgaa tcctctacgt
     4141 acgcctcgga cccctgatcg atgcggacga tgattgtggt gggcgtgaag gccggaatga
     4201 attccggcac gccttcgggc aggcccatgt cgagcgctcg gaacggcagg ttcagagccg
     4261 ggtcgcccag caggacaaac tcttcaaaaa tgttccgcgt atggtcgtgc gccggcccga
     4321 aatcgggatc ggccaggatt ttccagagcg ccgcctgcca agccggcccg acgcggcaga
     4381 tatgttcctc gaagaacgac tggaagaaat agcgttccat gcggcgcgac gactcccaag
     4441 cgccggaact gccccaccag accaggccgg acgacgacag ataggccgcg gcgcctttgt
     4501 tcggcgcgcg cagccatgtt tcgccgaagc actcggcggc ggtgtactgt gcactgttgc
     4561 aggaccagcc catcgccagt ccgtacaggc cgttgttggt gagcgagttc acgtccgatt
     4621 gatagaaggc cggcgaaaac cagccgcctg aagtgctgtg cccgaagtac accgtgaaga
     4681 gcacgccatc gttaacggcg gcggcgatgt cggcggtgcc gccacccaca ctgccgtaaa
     4741 cccgcgtggc ggtgaagccg gccggttcga agtaggtggc gatgatgtag ttgtgcagcc
     4801 catcggcctc ggcggtcggg tcgtcggtgg cgatcatgca ggcgcggcgg gcgtagtcgg
     4861 ggtcgctgaa tccgccggat tccacggcga ttgtcttctc gaccatgtgc tgcaatgcgc
     4921 tggtggtggt gactgaaaag cgtccgaaga acatgctggg gtaccagtca tcgccgccat
     4981 ccatacacgc gtagggcaag tcggtcgggc tatgccggct gccttgtccg atccaatgcg
     5041 gaatcgtggt gctgctcgaa gtttcaccgg cggtgtcgcc cacgattagc aggtaagcgg
     5101 gggcggcggg agtgccccac aggctctgga tgtaagcttt gatctcgttg cgagtggtgc
     5161 ccgagggcac gttgtagacc gacacgtcga agccgcgcgc ggtgcggtgg tctatgaaca
     5221 ggttcagcgc cgggctgtcg acgtagtttg gggctgaaac gatgaggtat ttttcattgg
     5281 cggccgccga cggcgcggcc caatacccgc cggccaaaat gacggcgata caactcagac
     5341 ggcagaggct cgctccggag tccttcatct ccgctctcct tcgggcgtcg caatcaggct
     5401 tgtgcctgct ttggggtttg gcccaggggc gaacgagact cggacgccgc gccgcgctgc
     5461 gcgcctaacc gcgaatttcg cgacaaccaa accgcgaatt cacaataaca tactcaacga
     5521 ccgaaaagca atgcatgttt aagagcagcg tgtttagcgg ctgaaaagca aagcacgttt
     5581 gacggccgaa aagacgcggg cggggcgatc cgagggcgat cgaacagttt gcaatcgggg
     5641 cctttcagcg aaattccggc tcgacacgag ccggctctgc tacttgagcc gggtctgcta
     5701 cctgcggagc cgatccgatt cggcgggttt tggcgcggtc cttccgtgat ggtcgccggc
     5761 tgggtatcct gagcggcctg aactgatcgg ccaccacgcg gcgtgcctat cgagcgggcc
     5821 gttgcgcctg gccagccaag aagggaaaat gcatatgcca cagcaggccc aagtcgaagc
     5881 ccaggagact cgaattccaa agtttccgga gaacgcctat cgggaactga agcccggcga
     5941 gaaattcgtg ccgatgatcc cgccgagcat gcgggtgccc gagctgacgc cgcggatgtt
     6001 catcttcggt ttgctgatga acgtcatctt ctcggtggcg gccaccttcc tggccttgaa
     6061 ggccgggcag ggcatcgaaa cggccattcc gatctcgatt ctggcggtgg gcttgtcggg
     6121 gttcctgctc aagacgggca ggcgggccag ctcgattctc gagaacatgt acgccctgtc
     6181 gatcagcacg acctcgggct acgtggccgg tgggacgtgc ttcacgatgc cggcgattta
     6241 catcctgaag ctgaactcgc cggaaagcct gaacatcggg aacctttcgc tcttcctgca
     6301 gatctcgctc gttccgtttc tgggtgcgat cctgggggcg ctgttcctga ttccgttcag
     6361 gcgctacttc gtaaaggaaa tgcacggcaa gctgccgttc cccgaggcga ccgcgaccaa
     6421 cgaaattctc gtgactggcg cctccggcag cactacgcag gcgttcatcc tgatttactc
     6481 cttcattatt tccgcgatct acacgttcgc agccacggcc ttgcgactgt tcggggacgt
     6541 gttcaccacc ggcctggcgc atctgaagcc cgtgggcaag gcgccgaccg aagtcctggc
     6601 tttcggatcg accgggctat tcgccgatct gacggaaaaa gtaaaggccg tcgtctcgat
     6661 gggcacgggg gcgtattacc tcgggctggg gttcatcatc ggtctgcggt acgcctcgat
     6721 cattctggcc gggtcgttgc tgtcggtgct ggtcatgacg ccactgctcg gctcgctgcc
     6781 actcgaacaa ctgcaaggga tcaacccgga cgtgaaatcg acgtcggcgc acgacatctg
     6841 gtacaacatt ccgcggcaga tgggcattgg cacgatcttc gccgccggcc tgatctcgat
     6901 cctgaagatg gggggcgtga ttgtgacggc gttgcggcag gccttcggcg tgctcttctc
     6961 gggcaagggg ggcggcacgg caacggaccg cacggatgag gatatcagct acggcgcgat
     7021 gttcgggatc ggcctgctgg tggtgatcgc gatggcgatt ttcttccgca ccgtggtatt
     7081 cgcccaaatg cccaacgcca cctggctgac attcgtctcg gtcgtgctgg cgctggtagt
     7141 gaccttcctg ttcacgacgg tgtccgcctg ggcgatcgcc atgatctcag tcacgcccat
     7201 ttccggcatg acggttacga cgatcatcat cacggccgtg ttgctggcgg cgttcgggct
     7261 gccgcgcggc gaaatggcga tgctgtcggt tctgctggtg ggcggcgtgg tgtgcaccgg
     7321 cttgtcgatg gcgggcggcc tgatcacgaa cttcaagatg ggttactggc tgggggccag
     7381 cccgaagcgg atcacctggc tggcgctggg cggctcgctg ctttcggcgg tgctggtgtg
     7441 cggcacaatc atggtcctga gttaccagcc cgggtacacg ggtgataacg cgttgcgggc
     7501 gccgcaggcc aacatgatgg ccggcgccct gcagagcttc ctggggacgg ggcagccgcc
     7561 ctggatgctg tatggcgtcg gcgtggtgct ggcgctgacc atgcagctct tgggcatctc
     7621 gccgctggct ttcggtctgg gcatgtacct ggatatcagc atcaacgccc cgatttttgt
     7681 gggcgccgtg gtggccacgc tggtcagcaa gagcacgcgc aacgagcagc tcgccaaggc
     7741 ccgcactaac aaaggtatcc tgctggcttc cggcttaatc gcgggcggcg ccattacgga
     7801 ggtgctggtc aacttcacca cggccattga tgaactcagc ctgggcgtga agaccctggt
     7861 caacgggacc gagaagatgg tcggcaagat catgccgttc ctggatgtga gtgggcggct
     7921 ggtcgacgcg ggcatcgacc cgggcgattt ggcgcgctac cagaactggc ttggcctgat
     7981 cctgttcctg gcgttgtgtg tgttcgtcta ctttgactgc cgccgcgcca agcccgagtt
     8041 ggggcagggc ggtcatttag gctagcctag gctcgagtta agtgagatgc tgattatggg
     8101 cggcgcgggc tgcgccgccc atcgtccata gagtgaggga aattgcgatg gcgtgcacgc
     8161 cagaacttca gacgaagttg cccgaaatcc agacaaagct gcccgagaac gcctaccgcg
     8221 agctgagacc gggcgaaaca tatgttccga tggtgccgcc caacgtgatc gtgccggagc
     8281 tgaccacgcg ctccatcgtc ttcggcatca tcatgaacgt gctctggtcg gtggcggcca
     8341 cgttcgtggc gctgaaggcc ggctcaggca tcgaaaccgc gattccgatt tcgatcctgt
     8401 ccatcacgct ttccggggtg ctgctgcggc tgggctatcg ccgcacgaca ctgctggaaa
     8461 acatcaacgt cctggcgatc ggcagcacgt cgggcattgt ggcgggcggc acgtgcttca
     8521 cgatgccggc catctatgtg ctgaagctga accagaatct gggcatggga gatatgcagc
     8581 tattcctgca gattttcctg gtgccgtttc tgggcgcgat tctgggcgtg ctcttcctga
     8641 tcccgttccg gcgttatttc gtcaaggaaa tgcacggcaa actgccattc ccggagggca
     8701 ctgcgactaa tgagattctg gcggccggcg ccgcgggtga gggcaagcag ggattgatcc
     8761 tggtttcctc gtttgtcctg gcggcggtgt acaacttttt gtgcacgggg ctgaagctgt
     8821 tcacgggcac cttcaccacc ggcatgctca atgtgagcgg cgagaaagtg ctcgcttttc
     8881 cgacgacgtt cttccacacg ctggtcgaaa aggtaaaggc cgtgttcttc atgggcacgg
     8941 gcgcctatta ccttgggctg gggttcatca ttggggtgcg gtacgcggct atcatttgcg
     9001 ccggctcatt cctgtcatgg tttgtgatcg tgccactgct gggcggattc agcatcgata
     9061 cgctgcacgg cttgaacgac caccccgagt tcaccgatac gacggccgag cagatcttca
     9121 aatttgtgcc gcgcaatatc ggcatcggcg ggatttttgc ggccggcata atttcagttc
     9181 tgcgaatggg caaggtgatt gcgacggcgc tgacgcaagc gctgggcggg ctgctgaagt
     9241 cgaagggcag cgcggggacg ccggaccgga ccgaccagga catgagctat tcgacgattc
     9301 tgctgttggg tctgctgacg agtgtggcgt tgtggttcta cttccggttt ttcgtgcttg
     9361 cggacatgcc cagtccgaat cgtttgacaa cgatcagcgt gttgattgcc ttgggggtga
     9421 cgttcctctt caccacggtt tcggcctggg ctatcgcgat gatttcgacg acgccgatat
     9481 ccggcatgac ggtgaccacc atcatcatta ctgccgcggc gctgctggcg gcggggctgc
     9541 agaagggcga cggcgggatg ctggcgacgt tgctggtggg gggcgtggtt tgcacggcgc
     9601 tgtcgatgtc aggcacgctg gtgaccgaat tcaagctggg ctactggctc ggtgcgagtc
     9661 cccggcggat tcaatggagc gcgattatcg cgtccattct cgcttcggca tgcgtgaccg
     9721 cgacgatcat gatcttcgcc cgcagtcccg gctacgtgcc ctcgcccgag catccgaatg
     9781 cgctgccggc gccgcaggcg aacatgatcg ccacggcgct caacagcttc ctggggacta
     9841 gcggcaacgt tccttggacg ctctacggtg tcggcggagt aatctcgatc attctgtgga
     9901 tgctcggcat cagtccgctg gcgtttgcgc tggggatgta cctgccgatg gagatcaata
     9961 cgtcgattct gctcggcgcc attgtcgcca agctcgtgga acgcggggcg cgcgatgagc
    10021 gggtcctcaa ggcgcggacg gacaagggga tcctggtcgc atccggcctc attgccggag
    10081 cggcgattct tgaagtggtg atcagtctga tcgggaattt cagttggggc gagaagttgc
    10141 tggccgccat cgacattaac aaacgtttca tcgaagccgg cgcggacccc gcacaggtca
    10201 tgcggacgca caattggatc ggtctggcgg cgttcttggc gctatgctta ttcgtgtact
    10261 ggaactgccg gcgggcaaag ccggagacag taggttgagg gcggctagat aggccagcct
    10321 ggccatggcc gttcctcggc aatcgcagga gaaagggaac gagcatggca ggccggtccg
    10381 agattcagac ccagcagtcc ggaagtatcg aggacaacct caagcaggcg gtagcgtctc
    10441 ccgcggccgc gcagccgcag gttgaccagt gcaaaacggg gcccgcggtc ggcatcccga
    10501 ctgtagccag tcccgcagtc ctcagtccca cggcagacgg tcagaccgtg ccgcgccctt
    10561 ctgcgcccgg tctgagtgca cgcgagttga gcgggcgggc gatcgtcttc ggtatgctgg
    10621 ctaacgttct attttcgatg attgctacat acctggcgct caagaccggc cagatcatcg
    10681 caacggcgat cccgatttcc atcctggcgg tcggcctctc gggtttcctg ttgcgcctgg
    10741 gattgcggcg cagcgcgact gccgagaatg tcaacttgct ggcgatcggg agtacagcca
    10801 gtatggttgc cgcaggcacg gtgttcgcga tgcccgccat ctacctgctc gggttgcagg
    10861 actcgctgga tctgggcagc ccgacgattt tccttcacat ctttctgatt ccgttcttgg
    10921 gggcgacgct gggcgtagtc ttcatggtgc cgctgcgccg ctacttcctc agacaaacac
    10981 gcggcgagca ctcctttccc gaggccgccg cgaccaacgc catcctggcc gccgggcagg
    11041 gcggcagaac gcataccttc gtttttgtgc tgtcctctct tctggccggc gcaggggcgg
    11101 tctgtgcgag catgctgcgg ctatggtccg agacgttcac gaccggcaag ctggacataa
    11161 cttgggtgga aaaggcggcc gcgggcgaca aggccggcca atgggtgggt cgcagcttcc
    11221 agagcctggc tttgccaggg agcccgggcg acctgtggta tagtctgaca gtcaagttca
    11281 aggcgttcat cgccatgggg acgggcgcgg aattcctcgg cctaggttac atcatcggcc
    11341 tgcgctacgc ggccgcgatg gtggcaggtt cgatgctgtc gctgctagtg gtcgtgccgc
    11401 ttttgtcgca gcttgatctc ggggctttgc agcagctcaa tccggcgatc aacagcaccg
    11461 cgagcgatgc gatcttcaac aacattcccc ggaacatggg tattggttgc atattcgcag
    11521 ccggcgccat ttgggtccta aagcgcagcg gggggatggc cggctcgcta ggcagcgcgt
    11581 tgatcgggcg cttccgccag cgtggttctt cggtggtgct cgagcggccg ggcaatgaca
    11641 taagctatcc ggtgctggga ctgatgggga tcgtcgccgc cgcagtgatg gccgggtatt
    11701 gctacttctt cgccttcaag ggcgtgccca acggcgcagc gctgacggtg gctgcggtcg
    11761 tttcggcgct gctgatctcg ttcgtctttt ctgtcgtttc gggctgggca acgaccaggg
    11821 ctggggcgcc gccgatttcg ggcgtgacag tcttggccat cctgatcgtg ccgctggtgt
    11881 tgttgagcgc ggggctgagc ggcagcccgg cgggccagct tggggtgttg ttggtgtgcg
    11941 gcgtcgcggc ctcggcgttt tcggtcgcgg gttctctgat gaatgagttc aagctgggcc
    12001 acgggtgcgg cgccagcccg aagaagattg cctggagctg cattcctgcc tgcgcgttgg
    12061 cctgcgccgt ggtcgcggcg acgatcatgc tggccgcgcg gagcggaggc tttgagggcg
    12121 gggcctcatc cacggttttg ccggccccgc acgcgaatct gatgaaaggc gcggtcctga
    12181 gcattcttgg cgacggagag gcgccgtggc tggcctatgg cgtgggggcc gtgattgcgc
    12241 tggccgttca ggtcgcgggc atctcgccgc tggcattcgg gctaggaatg ttcttgccaa
    12301 tggcgctgac tctgccgctg ctcgtgggcg cgctcattgc cgccgccgtg aaatggggga
    12361 cacgcgacga ggctacgcgc caggcccggc accataaggg cttgttcata gcctccgggt
    12421 tcgtagtcgg cgcggccctg ctgggcgtga tcaacctcgc cctgcggggt tgggcgaagg
    12481 ccgtgaacga gctgaaccag ctcgacatgc cgtccaagct gatcgggtcg ggcgaggatc
    12541 ctgagaaggt cgggcaactc atgaactggt acggcctgct cgcgtttgcg ctcctggccg
    12601 tcttcatgct tgtgatcgcc cgctggacac gccgcccgaa aaacgtcgcg tagcgcctgc
    12661 gcgccggacg tccggttatg ggacagttcg gccgcgccgc agcggtccag ggcgccagct
    12721 tcgaaactta atgccgcatc gcgcaagcta agcgctacgc gatactcgcg cgacattttc
    12781 ccacattccg ccggcccagc ttatacgctc gccccttccc cgcggccgca ctttcggcca
    12841 gactacttcg cacgtagtaa caccgaacat gaggaggtct gctttgtcga gattcgcgag
    12901 ttgcgccacg ctggtattga tcgtggcggc cttcgccgtg cccgctttgg ctgaaagcca
    12961 tctgatgcgc atggccgacg tacacgggga caagatcgtg ttcacctatg aggacgacct
    13021 gtggctcgtg ccgagcgccg ggggcgaggc gcggcggatc acgaatgatt ccggcacgga
    13081 aatctgggcc aagttctcgc ccgacggcaa gtggatcgct ttcaccggcc agtacgacgg
    13141 cggtgcagat atttacgtca tgcccgccaa cggcggcgtg ccgaaacgac tgtcgtttca
    13201 tcccagcaac gagcgcatgc tcgaatggtg gccggacggc aagtccgtca tcttccgctc
    13261 taattttgag tatccgttcc gcggcgagaa ggtgtaccgc gtttcgatcg agggcggtct
    13321 gcccgaggcg ctgccggtgg accgggccgg gctgacggcc atctcgccgg atggcaagca
    13381 gatcgcgtac aaccgcatca cgcgcgagca cgcgacctgg aagcgctacc agggcggcat
    13441 ggctcccgac atttggatgg gcagcctgga caagcacgat taccgcatca tctgcgactg
    13501 cgacttcagc tcagttttcc cgatgtggca gggcgacgcg atctacttcg cctcggatcg
    13561 cgagttcggc acgctgaacc tttacaagta cgaaaccaag gacggcacga tcaagcagct
    13621 cacgaaatac aaggactatg acgtcaagta tccctcgatc gggccagacg cgatcgtcta
    13681 tcaatatggc gaatcgctgc acctgctgga cctgaagacc ggcaagacgc gcatggtgcc
    13741 ggtcgagatt cccagcgacc tgacgcgcat gcggccggag ttcgtggacg tcaggccgag
    13801 cagcggggcg tttcgcctgt cgccgacggg caagcggctg ttgctggaag cccgcggcga
    13861 gatcatcaac ttcccggtcg aggacggtga gccggtcaac ctcacgaagg cccccggttc
    13921 gcgtgagaag gaatgcgcct ggtcgccgga cgggcgctgg atcgcgttcc tgtcggatcg
    13981 cacgggcgag gaagaggtgt ggctggtcga ccagaagggc gaaaacgagc gccagctcac
    14041 gcggggcggg ctgggcttcc gcatgcacct ggaatggtca ccggacagca agtacctgct
    14101 gttcgccgac aagttcttga agctcaacct ggttgacgcg gccagcggtg agctgaccgt
    14161 gatcggccag gctgattacg acgacgcctg ggagcgttgg ggcattcagg actatgtctg
    14221 gtcgccggac agccaatggg tggcgtacac gaagatggaa cagagcatga acgacgccat
    14281 ctttctgtac tcattggcgg agaagaagag ctatcgcctc accaatgatc aggcgacgag
    14341 ttggagcccg tctttcgacc cgcagggccg ctacctgtac tttttgtcga accgcacgtt
    14401 cgagcccatc atgtgcttcc aggatcagaa ccacatattc atcaatatgt gcaagccgta
    14461 cgtcgtggtt ctgaaggcgg acgacacgtc gcccttctat ccgaaggact cggccgaaga
    14521 ggtgaaggga gagccgaagc cgaaagatga ggctgcgggc gaggacaagc cggagaacgc
    14581 cgaggaatcc aagccggccg agaagaagaa aggcaagcag ggcgttgaag tcaggatcga
    14641 tctggacggg ctggagcggc gcgttttggc ggccgacggc gttccggccg gcaactactt
    14701 ccggctcgag gccacgaagg atggtttcct gtacctggcc aaggacgagc cggaatttga
    14761 gaagtaccag gtggtgaccg accgcacggg tggcaagctt gatctgtatc actacagcct
    14821 ggagaagaag gaagccaagc ggctcatcaa aggtattgcc aattatcacc agtcagccga
    14881 cggcaagaag ctggtttacc gggacggcag tacgtacggc gtcgtcgatg tgggcaagga
    14941 agccaaagtg ggcgacggca aagttgacct cggcaagatc aagatcgcgg tcgaccgctc
    15001 ggccgagttc ctgcagatgt tcgatgaggc ctggcgcatc cagcgcgact ggttctacga
    15061 ttcgggcatg cacggcgtgg attggccggc gatgcgcgaa aagtaccgca aattcgtggc
    15121 attttgcggc gaccggagcg atttgaatta cctgatcggc gagatgatcg ccgagctcaa
    15181 cgtcgggcac acgtacgttt ctggcggcga tctggagcgc tccggccgca gagcttcaac
    15241 gggcttgctg ggcgtggagt ttgcggccga gcccggcgcg aagtactaca agatcgcgcg
    15301 catcattccc ggcacgcccg gcgatccgca ggagcgctcg ccgctggatg agcccggatg
    15361 cccggtcaag gccggtcact atctgatcgc aatcgacggc gatgaggtcc acacgaatac
    15421 cagcccgttc gagttcttgc agaacaaggc gaacgtagtg gtaacactga cgtacaacga
    15481 caagccgtcg gcggagggcg cgcagacctg gcgcgtcaag acgatcgcca gcgagaaccg
    15541 catccgctat cgcgagtggg tcgagaacaa caaggcctat gtcgagaaga tgaccaacgg
    15601 cgaagtgggc tacgtacaca ttccggacat gggtgcaggt gggttgatcg agttcgcgcg
    15661 ctactggtat ccgcagtact acaagaaggg attcattgta gacgagcgct acaacggcgg
    15721 cgggtttacg ggcgacatga ttatcgaccg cctcgagcgc cggctttggg gcttgacgca
    15781 gccgcgtgaa ggcaagtgca tgcgcgaccc ggagcgctgc ttccacggtc cgtgggcggt
    15841 cgtcataaat gaggacaccg gttcgaacgg cgagttcttt gccgaggcca tcaagatcaa
    15901 gaagctcgcg ccgatcatcg gcatgcgcac gtggggcggg gcgatcggca tcgagccgca
    15961 ccaggacctg gtcgacggcg ggtcagtgac gccgccgcag ttcgggctgt acggcctgga
    16021 ttgcaaatgg ttgattgaag gccgcggtgt cgatccggac atcgaggttc agaacatgcc
    16081 gggcgacgtc ttggccggca aggatgccca gctcgacgcg gccattgaga acgtgaccag
    16141 gcgctaccgc gagaatccca tggatattcc gcctccgccg ccttaccccg acaagagcaa
    16201 gaagtaggac gtaaggcgac cccggggcaa cgaggcaata acgcaagggc caccgcttca
    16261 agcggtggcc ctgctgtctt caggcttata acggttgcgg tggaggggct tcagggctta
    16321 acggctttgc cgacggctga agcgtcgcga gttttgtcct gcgcttcggc cttgctgccg
    16381 gcttccgccc gctgcttgcg cgtggggatc gaatctagct tttccttgag ctgcgtgaac
    16441 atgttgtcca gcggccgatt caggcgctgc aactgttcct cggcccggtt gcgctgcgct
    16501 tcgctgcgcg gattggcggc cgcttgccgg gccttttcca gttcgtttgc attgcggcgc
    16561 aggaaagctt cgcgccgctc ctggagcgtg cgcaggtact tgtgggccgt agcctgttgc
    16621 tcggcgttaa gctggtagcg gcggatgaag tcctcgacgt acttttccca gtcatctccg
    16681 gccggccggc tgctgacggc cgagcggccg ttgtcgcgag cggccccagc ggcatcgccc
    16741 gccgcgccgc cggtttcacc ggttgatttc ggcacgccgc cgcgggccgc gatttccgcc
    16801 tcttgggcct cctgggccat ctgtctgttt cgcgcttcct cgttctcttt gaagccgggg
    16861 ctgcgatgcc actcggtttc ggcatcaaag ccgccttcgg cccagcccac caggcgttgg
    16921 ttgaggtact tcatgcccac gcgaaaaccg gccaactggc cgtccagcgt gagctgctga
    16981 tcctcggtca gaaacgggcg catctgctcg ctgacggttt cgcaccgctc ggcgaactcg
    17041 tctaccaggg gcaacacgcg ttgtgcccag tcggcgacga attcagggtc gggcggtttg
    17101 tcttccaaca gggcttcgaa gtactcgtta gtgagctgct gaatctcgtg gcgatgttcg
    17161 ttcagccagg cggggaattc gtctttccac agctcgcgcg tctggtgcaa ctgttcctca
    17221 ttgaagccgt acgtcttacc gagttcctcc gtcatgcgat cgatgaatag atacatcatg
    17281 cgatcggtcg gccagaagcc ggctgatggc gggggcggcg gtgcatcgaa gtcacgggca
    17341 tcatgctccc gaggcggacg acgcgggccc tgagacagcg cagccggcac aaacagaacc
    17401 aggcacagca gcacggcaag tcttttcttc gcacccatgc agaactccct attaccacgc
    17461 cggctgaaag cgtaattcca gaaccgctcg cacgcgatac ccagcggacc gcaccaacac
    17521 gcgggcagcc gaacagcggc cggcgcgcac gaccgccgtc cattttaccg gccaggcatg
    17581 tccaggttac cgcccgacgc gagtttacgt tagccgacaa gtcctgacag aggcccggca
    17641 gcgctatata ttgcggtcag aaggccgagt ggcgcggcct gtgcgcggag tattcctcga
    17701 tgggcggacg acgcctgatt ttggctgcgg cgctgctgct gcccgtgctg ccggccggtt
    17761 gccaagtcga cccgggttcg ttgctgccgg ccgagcaggg tcaggaccag agccccgagg
    17821 gcctgcggct ggtggcgcgg ttcgcccaca taaccgatac gcatttgaat gatactttgt
    17881 cgccggcccg gtttgccggc gcccacgccc tcacccgttc ggcatggcgg ccttacgaag
    17941 catattcgac gcaactgatg gacggcctga tacgcgcggt gaatcgcatc cacgcctccg
    18001 gcacgcgggt tgatttcctg ctgcacaccg gagacgcggg cgaacaggcg caatcgaacg
    18061 aactgcaatg gctggtgcag atcatggacg gcgggctaat caacccgctc tcggggcccg
    18121 atgaccgccg cgcggaccaa cgtccgccgc cggagctcga tccgtacgcg gtcttcgtcg
    18181 cggaggggct ataccgcact ggcgcacacg gcgatgcgcc caccatcccc tggtacacct
    18241 tgttcggcaa tcacgacgtg tacgcgctag gcgtatttcc gatcttggag gtcgaaccag
    18301 gccggctgct ggcgccactg ccagcgcccg atcgtcccgg ctggctgttg cccacatggc
    18361 tcgatccgct gtcctccttc acccacgggc ggatcacgcc tgcgcacccc ggtccaccgc
    18421 tattgcttga gtgccccagt tacctgcaac cgaacccgga gcgcgcttac ttcggcaaag
    18481 cgcatctaat tgcgacgatg ttcggcacgc tcagcccgcc ggccggacat gggtttgcag
    18541 cggccgacgg gccgacgtgg tacagcctaa gccccgcgcc tggtctgcgg ctgattggcc
    18601 ttgataccac ggaccgtacg gcccagttcc cgggtcagct ttgccacgat ggtgcggtgt
    18661 cgcgggcgca gcttgaattt gtgcgggccg agctggaggc cgccgcggcg cgggatgaac
    18721 tggttgttgt ggcggggcac catccgagcc agacgatacg cgccgacttc ggcgtgcagg
    18781 tcacgggcga ggagctgcgg gaggtgctca agcagtacgc caatgtggtc ctgtacatct
    18841 gcgggcacgt gcatcgcaat cgcgtgattg atcgcggggg gtacttggag atagagactt
    18901 gctcgtcgct tgacctgccg caggaggggc ggattgtcga gatctggcgg gatccggccg
    18961 ggccgcgcat cgcgataagc taccgcacgt tcacgcacga cggggacgat gcggatgaca
    19021 cgctgctgcc gctgcgcgcc gccgcccgag ccatcgcgct ggcggacaag ggcgccgcgg
    19081 cacgtcagaa agagcgcgac cccagtggtg tacccccgcg cggcaggccc gccgaccgcg
    19141 aggggtggtt cattcggtag cagagccggc tcgtgtcgag ccggaatgtc gccagaacgc
    19201 ggatcgcacg gcttccaccc ttcccactcg ccaccttctc acctgcacac aacatctggc
    19261 ggcatgcccg cgcttagagc ttgggcatgg cacccttgcc gcgtatactc gcggcatgca
    19321 cgagcagatt cagtattaca gcacgaatcg ccaggtggcg ccggtgagct tggccgaggc
    19381 tttgctacaa ggccaggcct ccgaccgcgg gctttttatg ccggaccgct ttccgccgct
    19441 ggacctcgct gggctggcca agttgcgggg ccgaccctac gcggacgtgg cttacgaggt
    19501 gttgcggccg tatgcggatg gcacgttcga cgacgcgacg ttgcgggcct tgtgcgatga
    19561 cgcttacgac tacgaggtgc cgctggagcg cgtcaccggc cggcgttacc tgatgcgtct
    19621 ggatcggggg ccgacggctt cgttcaagga cttcgcggcc cgcatgatgg cccgctggat
    19681 gagtgtgctc atgcgcgagc aggcgggcga gttgattatt ctgaccgcga cctccggcga
    19741 tacgggtagc gcggtcgcac atgcttatca cggcgtgccg cgcgtgcgtg tggtcgtgct
    19801 atttccgccg gccgaagtct cggatcggca gcgcaagcag atgacgacca ttggcggcaa
    19861 cgtgaccacg atcggcgtcg acggcaaatt cgacgactgc caggcgctgg tgaagcgtgc
    19921 gttcgccgac ccggccttgc gcgacattcg gctgacgtcg gccaactcga tcaacatcgg
    19981 gcgcctgctg ccgcaatcgg tctactacgt ttatgcgtac atcaatctgg cggacgtagc
    20041 ggcccgcgag ccgatcgtgt tcagcgtccc gtcgggcaat ttcggcgaca tgatgggcgc
    20101 ggtgattgcg aggcgcatgg gcctgccaat ttcgcggctg gtgatcgcca ctaacgcgaa
    20161 cgatgaagtg ccgctattcc tgagtagcgg caggtatcgg aagatttcgc cgtcgcgcgt
    20221 gtgcatctct aacgcgatga acgtgggaca tccgtcgaac ctggcgcggc tggtggacgt
    20281 ttacggcggg atgatggacg agaccgggct gctgcgcgaa cagccggata tggagcagat
    20341 gcggcgcgat ctgtacgcca tcagcatcag tgacgatgaa acgcgcatga tgatccaggc
    20401 ggcttacgag cggcacaaca cgctgctcga gccgcatggc gcggtcggct gggctgggct
    20461 ggaagcctac gtaagcgaga acgcctccgc cgcggaagcg ctggccgttt cggttgaaac
    20521 cgcacatccg gcaaagtttc cagaggaaat tcgttcgctt atcggcttgg acccgccggt
    20581 cccgccgagt ctggccggac tggagacccg gccggaacag tatgagcgga tgagcacgag
    20641 ctatgagccg tttcgggatt ggctgctggg ggggttcaca tgagggcagg tgctaaacca
    20701 taacccgcgc cggccgctac cggatatcat ccgccttcat gagccagctt tccgccgcct
    20761 actggccctc gatcgcccca gccgaaacgt cggctgcgcc gctggtctgc tcggccgtcc
    20821 tcacgcacca ctggctggtg cgcctgcgcg gcggggagaa ggtgctgcaa gcattgtgcg
    20881 aattgctgcc cggcgccccg atctacaccc tcattcacga cccgcaggag atttgccggg
    20941 gctggccgcc ggtgcgagct tcaattctca atcgcctgcc cggcgtgcgg cgctactacc
    21001 ccaagctgtt ggccctgttg gccctggccg cacgttcaat ccggctgccg gaggtcgatc
    21061 tagtagtttg ctccgatgcc gcggtggcta aagccatgcg gccgcacccg cgcagcaggg
    21121 tggtctgcta ttgccactcg ccgatgcgct atgcgtggga cgagcagttg cgccggcagt
    21181 actgtcggac gctgccgttg acgcatgtta caccgtttct cctctcgacc gcgctggcgc
    21241 ggcggattga tgcggcgggg gctcggcgcg tcgatcagtt cgtagccaac tcgcggcacg
    21301 tggcggcccg catccgccgc gactatggcc gggaaagcgt catcgtttac ccgccggtcg
    21361 atctgccggc cgagccttcg cgcgaagcgc gggaggattt ctacctgtgc gtcgggcatc
    21421 atgtgccata caagcggctg gatctggccg tggaggcgtg cacgcggctg gggcggcggc
    21481 tggtcgtgat cggcgagggg ccggaggtca agcgcttgcg cgggcaacgg ctgcgcggcc
    21541 agggcgacgt cacgtggctg ggctggcagc cgtccgaggt aatcgcggag tgttaccggc
    21601 gggcgcgcgg gctgctgttt cccggcgaag aggatttcgg catcgtgccg gtggaagcta
    21661 tggcccacgg ctgcccggtg gtcgcgtacg gagtgggcgg ggcgaccgag acggtcagcg
    21721 acggggtaac gggggttttg ttcaaagagc aacacgcgga tgcgctgatc gaggctatgc
    21781 ggaaagtgga atcgaccgtg ttcgacccgg tggcgatgca cgccgcgatg cagcgcttca
    21841 gtcggcgacg ctttcttggg gaaatggggg cggtgctgcg cgcagtctgc agtgataaag
    21901 tgataaggtg atagggtgat aaaggggtcg cgggccgacc tatcacccta tcactttatc
    21961 acgttatcac ctcatcacgt cgtccgcggc gctatggcaa atcgttggca gtgacgattt
    22021 tgcattgtcc cccgttttgc tcggcgatgc gccgcaggcg gcgggcgcct tcggtttcgc
    22081 gcggcaacct gctggtgtct tggagcagga tttcgtcaaa aaccaggcac accagttttg
    22141 cgccgacgct cttgttagcg cgggtcagtt catcgctcac ggaatcgtca aaaaagccgt
    22201 cggagaagaa gaaaatcgcc tgcggcttga gcgccagggc gcgtttcagg gcgggcagcg
    22261 ggtcggttgc gccgcgcggg gatttgctgt ccagccactg gaaaaacttc cggcgcgttt
    22321 cttgagtcgc gggctgaagc tgcgagccga acgcgtccgc tttggaaagt tcctgcccgc
    22381 cggcgccttc gccgtaaaac aggatgactt caaaaagctg cgtcgggcgc aaccgtccga
    22441 tggagtgctt caattcccgc ttgagttcat cgaccgcgac gatcaccgag ccggagtagt
    22501 cgatgacgta cacgatgcga ttggccggga cggagacgtt ccacacgccg gcctgcccga
    22561 ggtcgccgcg ttgtccgaag gagctgccga agccgccact gccagccccg ccggcgacgc
    22621 cgccaccggt gccgagcagg ctcagcgagc ctgtgccaag gtcacccaaa cccatggcgt
    22681 ccgaggctgg gccgaggttt gggccggttc cgagcgcgcc gggctcgagc ggcgtgacgc
    22741 ggctgaaatc caggctgcct aagtctggga tggcggtcgg cttttccagc tcggccagcg
    22801 gggtgggcgg ggtccagtcg agcgccggca aggtgttctg gtccggcgcc tggatgacgc
    22861 cggcggcata ttcactgaat tctgcagcgt gtgatgcgac gacctgccac gtcttcaacg
    22921 caagcagccc gaacagcagg atgtgcaacg ccaagctgat cgaggccggc aggcccaggt
    22981 gtatcagcca gcgatagccg cggctgcggc gttcgcgatc gggcgcggcc tcgaattcag
    23041 gagttttgca ctcggagtgc ggcggggatg cgtcggaagt ctgcattttt gatcctgcat
    23101 ccttaattca ggccgccaag ctgttagcgt gggcatgccc gcgcttacag cttgggcatg
    23161 gcacccgcca tcacacacac cattacaccc gcggtgacac ctgccgtcac gcctaccatc
    23221 acatgcacta tcccacccgt caccgcagct accgtcacgc tcccggggcg gacggcgcgg
    23281 gaccgattgg accgccggcc atttgcacgt agcgcatgcg catctcgtac agggtcgcgt
    23341 ccaacgcgcg tttctcttcg ggcgtcaggt tgttcttggt cttttcctcc agcacttgca
    23401 gcatgtctac gaagtgtttg gcggcctcga gattgggcgg aatgcgctcg ccgcccgggc
    23461 cgcccaccaa gccgagggcc accatcgcct gaatggcgat caggttcacc agttcagcga
    23521 acgacggctc cggcatgagc tcgtgcgccg cggcttgctg ctgttcggcc agccgctcct
    23581 tttcgcgccg cgcttcagct ttccagtcgt catcgacaat gatctttttc tgctcatcgg
    23641 gcatgaaatc actccaagcc ttgcgatcag cggattctag tagcggcagg cgcgcggcac
    23701 aacgcggcat ggacggtgga cggtggatgg tggagtccgg ccgggcttga acgcgggagt
    23761 tcttgccggg ttcagcagcg attataaaag ttgccgggca accggtggtg gctagccgcg
    23821 gtcgcccggc ttcgcttgaa ggaacacctt caaagcggat gcatcaatcg cttgtgaggg
    23881 catcgacaaa ggggtcgatg tcgaaagcgt tcacggcccc gtcggcattc atgtcggcgt
    23941 acatgatgtt gcaggacgag tacatctcca gataagcctc cgcgtcggtc agggccagca
    24001 cgaaggggtc gatgtcgaac gtgttcatct tgccgtcgca gttcatatcg ccgcgcgctg
    24061 tgcggtaggc acggatgtcc gtatcgacgc cacacacgat cagggtgccg ccggcgccga
    24121 gcgcggggcc gccaatgtaa atgtccgtca cggaagtctg ccataggagc gtcaggtcgg
    24181 catcaaacgc gtagagtcgg ccgtcttcgt aactgccatt ccccagatac acgcggccgg
    24241 cggcatcgat ggccatgcgc gcgatgaaga tatctggcga aggaatgtag cctgcgctgt
    24301 tgatgacgct gccgtcggcg ggattgaggc ggacgaactc gtagccgggt tttagcatgt
    24361 agattgagcc gtccggcccg atgccgaact ccgaggccac cgtccaggcc gcgggcacgc
    24421 tccatttctg ggttatcgcc gagccggtat ccgtgaaggc gtagaagtag tcatacgagg
    24481 gattgttctg ggtgcgcgac aggtagatgg tcccgtccgg accgcaaaac ggcgtgttct
    24541 gcataacaag gcccggcatt gtatcgccgg tgtattgcag ggcgcccgtg gccaaatcgt
    24601 agcgcacgat ggcgtggccg ccggaggcca ggtcggcgat gtaaacggcg tcgccataaa
    24661 gggccgcgcc gcaattgccg gcggcggaac aaatgcggct ggagtgccag acggtggcgc
    24721 cgtcttcgcc gctgatgcgc cagaggtcgg taaagctgcc gatgatcaga tcgccgttgg
    24781 gcgcgaagac gacgccgtcg tagggccgcg catcaatgtc ttcttcggag gtccagagga
    24841 tctgcccggt ttcggcgcta tacgcataga gcggggcgaa cgaggcgccg ccgcgcgaga
    24901 catagacctt tccgtcgttc acgccggcaa cccacggcac ccaaccgccg ggatatggag
    24961 ggaccgcccg gacccatagc tccgcaccgg tgtggatgtt catggccacg aggaacgcat
    25021 cgttgtgcga ttcgcccggc caggtctgct ggcgaatggt gaagaggcgc tgcccctcaa
    25081 tgaatgccgg ccaggcgttc atcgaatatc gtccgccgga ccaggccaag gtgggcgagt
    25141 ccggcccgaa atccgcggat tgtccgttgc gttgggggtt ctttccggaa gtcttccagt
    25201 cgtccgcgaa cgctccgcag gcgagcgcgg acgacacaag ggcagcgaac aagatcaatt
    25261 tgttcgcagt catgtgcctt ttctccttcc tgaatgtcct aaatatctgt tttgcgagtt
    25321 accgcttttg agagtacggg gtcgaactcg ccacctgagc ttaagtgtaa cccaattatg
    25381 ggcagccggg gctgttttcg ccgcttttcc aaaaagatga ctttctagtc ttccatttgg
    25441 ccggcacgga ccgagaacac gaaggcatgg cgcctggcgc gccaggaata tgtggccgaa
    25501 ggtagccgcg ggcgtccaaa ttcgcgttcg ataggtcccg ttgtgggagc gaagctcgcg
    25561 ccgcaagccc gtgcgccgcg ccggggtgtt ggggtatgaa gggtgaaaag ggtgcgaagg
    25621 gtgggaaggg cgttaagggc gggaaaaaag agaggctagc gcgcggcgcg gttgaagcga
    25681 cggcatacgt gttattggcg atacgcgagc aatattgagg gtgtgccggg gcgatacgcc
    25741 ccggctcaaa accggaaaat ggcggctgct gggctgccgt tgggtaggtc ggttgaggac
    25801 accgcatgca cggtgccgcc gtgcttaagc gtgtggatgg ccgccaagtt cagtaggtct
    25861 tcgttttgag gcgattgttt ggggtcgacg tgcagctcgc ccgtctcggg atcaaaacgc
    25921 ccccactggt ggcggtcgat ggcaatgctc agttcggcca cgcgcgcgtg catcgcggcg
    25981 gagaggattt ccgcgagatc ggtcgagccg cgatttttcg atatggcctc gggcagtagc
    26041 tcgaggtgtt tcagctcggc ggcacgaaac gcaggctctg cgatcgccca ggcttgagcg
    26101 tgcagttgct gaagcggcgt cggttccggg ttaccctcaa tgccctgcgg cattagaccg
    26161 gcatagcggc tgacggagcg gtacgtcggc tggagcgagt ccgcggccgc caggatcaag
    26221 ggctcctggc gatcgcccaa gaactggcaa acggcggcgt caacacggct gcagaaatgc
    26281 tgcagccgtg ctatttggtc cgagcccacg gcgctctgtc cgaagaagat ggggccgcgg
    26341 cggcctgact gcacgggggt gcgtgtgtgc cattgcacgc ttctttcgac ctcggtcggc
    26401 gatacggctt catcgatgtt ggccggcaga ttgggaacgt ggacaggccg cagctccagg
    26461 cggtccccct cgtagaggcg cacgtcgttc tgcgccaaag cgagaacgta aaaccggcgg
    26521 tcttggttga ctccaggtat caggggcttg atgtgaaagc gctcgccgac gacggatagc
    26581 ggatcgcact tgtacgggag ttggaattcg tagagctgat tcggagcgat tagtgccacc
    26641 aaggccgcat cgcgcgggtg agagtcgcgc gccgtttcca gcaccgggcg tgccgccttc
    26701 agcaacttgt cggcctccgg ccggcgaaag ccgcgctcca gcaacgcttc ttcggcctgt
    26761 cgcaacaggt tttttacgcg gatgcggctt tcctcggttt ccgggccggc ccccagtggc
    26821 acgtaaaagg aaacacacgg ttcactgttg ccttgaacga gctcgcgcaa ggtttgaact
    26881 ccgaacgcca ccatcgcgct atccaggtga aaggggctcg cattacagat tctacgccgc
    26941 ccgggcgcgc ggtccgcacg aagaacggat cggcgccctt tcggcctcaa gtggtccgat
    27001 ctgagacgat tttcatttcc gcactaccga agtaagcgct ggacctgggt cagtcgaact
    27061 ggtagagttt ggcttgggtt ttgaagcgag gagacgttgg tcatggacga cttgctggtg
    27121 cttgatcgcg tggaatccac agcgtctgac tgcacggggt ttaccgttct gaccgaagcc
    27181 gaaaacgcga gcgatgacgc ggatttcgtg cccgatgcgc tgaccgcgga ttcggcgcag
    27241 gagccggagg cgtccgcagc caccgctgac gccgagccgc cccagcctga ggcggctgag
    27301 gagctttccg agccgcgcac ggcggatgcc ccgccgacgg accagatcat cgagtcgctg
    27361 ctcttctcgg cagacgcgcc gctgagcctg aaccgcctct cggagctggc cggctgttcg
    27421 gtgatggaag cgcggttggc gatcgccgat ttgaacgcgc gctacaccgt ggccaagcta
    27481 acgttccgga tcgaggaaat tgcccgcggc tatcagatga tgaccttgcc gcgttttcag
    27541 ccttgggttt cgaagctgat ggcgcagcgc gctcaaacgc gcctcagccc ggcggcgctg
    27601 gagacgcttt cgatcatcgc ctacaagcag cccataattc gcgcggatat tgaagccatt
    27661 cgcggcgtgg cggtgggcga agtgctgaac cgcctgcgtg agatgggcct ggtgcgcatc
    27721 gtcgggcggg ccgaagtggt tgggcgtccg ctgctctacg gcactaccaa gaaattcctc
    27781 gacgttttcg gcttggcgga tctcgaggat ttgccgccgc tggaggcgct aaacctgcgg
    27841 cgccgaaagc cggcggagga tggcggcgcg gccgatgagc agagcgcggc accgcccgca
    27901 gagcgcctgg cggccagcgc gtagcttgga gatcgcgggg tcagggcttg gggctgaaga
    27961 cgtatcgggg gtttcccggg ggcatcgctc tttctttgag ggcttggttt ttccgtataa
    28021 tgcgaacgtg cgggagtctc tgcgagggta gatgccggaa gttaccaagc catcgatcga
    28081 agaatccggc gcgtcgcgcg cggcttctgt gacgcgtgcg cgagcgttgg cgcttcgcgt
    28141 ggacgagctg gggcgtaacc gccctgatct ggtcctaatt ttgccgtaca tggtgtattt
    28201 gctgctgctg gggctggtcg ggctggtacc ggccaagtgg gagtgggcgg cgatcgtcgt
    28261 gcgcggcgtc gggtcgctgg cggtagtcta cgcgtttcgg cgccacttgc cgcctctggg
    28321 gtgcccttat tggctgatcg cggtggcggg tggagtcttg gcggcgtggt tgtggtacgc
    28381 cgggcagtac gtgtttgacg cgtgggggct aggcgggcga ctgcctctgt atcctggacg
    28441 gaaagaggtc ctggacccgc gcgacctgct cggggcggga cggctgtttt ggacgacagc
    28501 ggtgctgcgt gttgcggtcg cggttacggc ggtgccggtc gtcgaggagc tgttctggcg
    28561 cgggttcatg ctgcgggcgc tgctcaattg gaacgatttc gagcgcgtgc ccgtcgggac
    28621 gttcggctgg acggcgttta tcggcactgc tttgctgtcg acgctgcagc atccggacaa
    28681 ttggggcgtg agcatcgcgt gctggttgtt ctacaacgcg ctgttctact ggaaacgcag
    28741 tctggcctgc ctgatgctgg tgcacggcct gaccaacctg gtgctgtact tccacgtact
    28801 gcgcgtcgga gattgggcat tttggtagat cgcggggcgg accgcgctta tggctgcgtg
    28861 cggtggcctg cggggttggg tccggccgga gccatccggc tctacggctt gagttgggaa
    28921 gggggcgcga tataactatg cgggctgccg tggaccgggg gctaccctgc gtgcctcgga
    28981 ggtgcacagt gaaacgagtc ttgttaggcg cagtgttggt gggattgctg gtaccgctcg
    29041 cggcgcgcgc ggatctagaa gagggcgcct tcgcgccgga tgtggatgcg gtcgactggc
    29101 tgaacacgga cggcaaatcg ctgtcgatat ccgagctgcg cggcatggtg gtggtgctgt
    29161 atttctggga gtcctggcag ccccagcaga gatacttgct gcgctcgagc aacttcgcgg
    29221 aaaaccagct tcgccaggcg ggcgttttct tgattggcgt aacctcggcc ggccgcagga
    29281 cggttgagga cctgatccgt caggagcaca tattctttcc gatcgcgttg ggctcgcgcg
    29341 cggcggaagc gtacaaaatc gagcccgagg atatgccgcg tgtcgtgatt attgatccga
    29401 gcggcgcggt cgtgttttct ggccctccta atgtgggcac ggtaactcaa aaggtgcaaa
    29461 aactcgtttt cgaggaggcc ccgccgtttc ggacgcatcc gcgtcacgcc gagaaagcgc
    29521 gcaaggcgtt ggaggccgcg cgcgaagcct tgaagcggga ggactaccag gaggccttcg
    29581 tcaaagcgcg cgaagctgag gacctggcct tggcggatga ccgcttgaag ctgcgttgcc
    29641 aggagatgat tgatttggtg gacgccatcg ggcgggatcg gctgcatcaa ggcttggcgc
    29701 tcatcgagac gctccaatac gaagagggcg tgaaggttat caatgaagtc ataaaacagt
    29761 ttcaggtggc cgggtgtggc aaggctgccc ggcgccggtt gcgccttctc aaagaccagt
    29821 atccgcaggt gcgccaggtt gcggacaagc tagggcgcga agatgaagct cacacgaaac
    29881 tggtctccgc ggccgagaag ctgtggcgac gcaagttcgg cgaggcgtat agcgcgctcc
    29941 agaaaatcga ggaggaatac agcgggacca aggcggctga gaccgccaag gtcatccgcg
    30001 atcgcatcga cgcgaacaaa acgctcaggc aaatcgtgct tgacaatgac gcccggaaag
    30061 tctgcgaaga ccggctggcg cgggcgcgca atttcatcga gacccgccga tacgaggatg
    30121 cccgaaaaac gctccgcagc atcatcgatg aattcccgca gaccagctac gtggaggagg
    30181 cctacaggct attatccgaa ataccctgag aacggtcggg ccgcggcccg gccgcgtacg
    30241 gacgtgaggg accctcgagg gtcatgaaca acaaggatcc gtatcagatt ctgggcgtcg
    30301 cccgcaacgc cagtcccgaa gagattaagc gtgcttatcg gcggctggcc aaagagtacc
    30361 atccggaccg gaatccgcag aacaaggagg ccgccgagcg cagattcaag gaagtgcagg
    30421 cggcgtatga ggtgctgggc gacccggagc ggcgagctca gtacgatcgc ttcggggcgg
    30481 gcggtccacg accggagttt cacaactggg gccgtggccg gccggccgaa cagcccttcg
    30541 gggtcgagtt cgatttcggc gatctggggg atttgaccag cattttcgag cagtttttcc
    30601 aacgtgggcc cgggcgcgca ggtgagcggc gtggggcgcg gcggacgcgg gtgcgtgggg
    30661 ccgagctgcc gcgcggcgcg gatatcgagt acccggtgga gatctcgttc gacgagtcgc
    30721 tgcacggcac gaagcgcgag gtggtcttac agagccccga aggcaacgag cgcctcgagg
    30781 tgcgcatccc ggccggcgtt aacgacggtc agcgcatccg cgtgccgggg cgcgggcagc
    30841 cgggtgcggg cgggcgcggc gatctactga ttcgcgttac gatccggccg cacccgtact
    30901 accggcgcga gggtctggac gtgctgatgg atttgccggt cacgctgacc gaagcggcgc
    30961 tgggggcgcg cgtggaactg cccacgccgg aggggcggac gattgtgtcg gttccgccgg
    31021 gcacggccag cgggaccaag ctgcggctgc gcgggcgcgg agctcacgac gcgcgccgcg
    31081 gcacgcgcgg cgatttgctg gcggcggtgc gcattgttcc gcccaagacg cttaccccgc
    31141 gggcacgcga gcttctggaa agcctgagcc gcgaggttcc gcagaacccg cgcgaaaagt
    31201 ggggcggtta agggaaattg atgccacaag cccgccgcag tctctccgga cgaatggcag
    31261 tgctgctgat cgtatacgcg ctgctcttgc tggtgtgtgt ggcactgtat ttcttcatca
    31321 ccaaccggct tgcggccagc gtggcgcgca tggccgcggg cgaagtcggc cgggaagttg
    31381 taatagccga gctttcgcgg ttggtggcga tcatgctggg ctgttttctg gcaatactgg
    31441 cgtttgcaat tggggcgtac acattcatcg ggatcgggcg cgcgatatcg cggcctacgg
    31501 ccagtggcaa gcctacgaag tatgtagatg cgtggagtca gtaccgcatt tcggaggaac
    31561 agattcgggc ggcgacggag gaggtggatg accgaaatgc gactgaggac ccgtcgtctg
    31621 ggcgcgacga cgacgatccg agctagcctc cacagtctgt tctgacgccc tgcgccgccc
    31681 tccgcgttct tcccgcagca tccgagcggt ccacgcatgc ggcgacggcg atgtcgcccg
    31741 tgacgttcag cgtggtgcgg cacatgtcca gcaggcgatc aacgcccagg atgatgccga
    31801 tcgactcggc cggcacgccg accgactgca gcactacaac taccagcggt agcgagccgc
    31861 ccggcacgcc ggcggttccg atgcccgcga gcacgcacat gatcgcgacc ttgatctggt
    31921 cggccagggt caagtccacg ccgaagacct gcgccagaaa caggaccgtg acgccttcgt
    31981 aaagggcggt gccgttctga ttggcggttg agccgaccgt cagcacgaaa tttccgattt
    32041 cgcgccgcac gcccaggttt ttctccgtga cgcgcagcga aaccggcaac gtggcattcg
    32101 acgatgatgt gccgaacgca gtcaggatcg cgtcggaaac acgcccgtag aaccgcaatg
    32161 ggttgatgcg cgctaccaga gccaacatca gtgagtacac gccgaagaag tgcagcgcca
    32221 ggccggccag caccgtcgcc atgtaccacg ccagcatttt taatatgtcg gctcccagcc
    32281 gggccgaaag ggcaaaaacc aggcccgcca ccgccagcgg ggccaggcgc atggccaggc
    32341 cgacgatgac cattacgacg tcgtagattc cctccagcac ggcgatcagc ggtttggtgc
    32401 gctcgggcgt gagcgtcagc gcgatgccga agatcagagc gaagaacatc accgccagca
    32461 tgccgccgcc gggggacgag ccgtccaccg ctcccaccat ttcgcgcagc agattccgcg
    32521 gaatgagatc gagcagcgcg tcccgcaacg gcttggcctg acgcgcctgg gccagctcgc
    32581 gcccggcctg ctcttcatat tgggcgctca aggcggcccg ctgctcggct gaaatacgct
    32641 cgccgggccg cagggcattc gccaggctga cgccgatgat gaccgaaatc gcggacagac
    32701 ccaccgtaaa gaccagcgtc atcaggccga cgcgccccag ccggcggacg tcgccgatgc
    32761 ccgccacgcc caagaccagc gccgagaaca ccagcggcag cacgaccatg aatatcaacc
    32821 gcaggaagat ttgtccgagc ggttccgcaa cgtactcgat ggcccaggtc aggtgcgggc
    32881 ttgccaagcc gtccggcccg tggccgaggc ggttcgcgac caggccggcg acggccccaa
    32941 cgatcaggcc gatgagaatt tgccagtgta aagcgggcca gcggcggcgg tttttggcct
    33001 gcgcgccggg ctcggggagc gttgtcggcg aaaaacgatc cggctctgtc atgaacgcac
    33061 cgccgtatca gaggccgcgg caattatcga ccggtcctca ggggcggcaa gccggcgcgc
    33121 gtgagaaggg ggtgaccgcg gttgcggttg gcgtgcggtt agttcttcac ccgcttccag
    33181 gtggcgctgc ggacgcgata ctgctcgcca ttgacccaga aagcccagtc ctgctgtatg
    33241 cgatcgtcgc taatgaacgt gatactcgtg ttgtggcaat ggcggttctt ggccacgtcc
    33301 aagttggtgc tgtcgtggta gacgaacgac aggcgctgcg gatcatcaaa gtttgtggcg
    33361 cgcatgcgcg tttggtggtt cagcgggcaa taatgagtga ccaccaaagt cgagtcgccg
    33421 tccatgtgat acatgtttac gtactcacgc accgtgtcgg ggaagtcggt ctgcagcacg
    33481 gtcttgccgc cggcggtgac gcgaaatacg gtcttgaact ccttgattcc ttcagggacg
    33541 gccagcggcg cgttttccat ctcccacgtg ccggccagcg acttgaggcg ctcaaacgcc
    33601 tgctccgcgg gggtgggcgt tttgacggtg atgttttccg gcgcaagtat ggtgggttgg
    33661 ctggcggcgg ccggcatgct ggtggcgggc tgggtggtcg cgccgggcac cggctcgatc
    33721 ttcttgatgc gcaattgcgg tgcgggcgag agaagcggcg gcgtctgagc cggcgcactg
    33781 gcggccggag cgggcgaatt cgccggttgc gcgggttcag ccggttgcac cggttgtgcg
    33841 tttcggcccg gttg
//
LOCUS       DAOSYS010000009        31315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1180_length_31315_cov_5.235285, whole genome shotgun sequence.
ACCESSION   DAOSYS010000009 DAOSYS010000000
VERSION     DAOSYS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 31315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31315
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1180_length_31315_cov_5.235285"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1199)
                     /locus_tag="PLU99_01170"
     CDS             complement(153..1199)
                     /locus_tag="PLU99_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15693.1"
                     /translation="MKVRRHADTQVVRGTAARRGLVALWPALPPRRSSVAAGYRRLCS
                     TPAFTIVELIVVMSVIVLILAIAIPGLNAIAAESRFSAAVQITNGALTHAYTAALADL
                     NMTAVRFVPGKWDNDPSTSESGTTGRQHIVTYSFTPTSAADPVHPGQVVFGEYFQRRP
                     GTSSVILPEDVWVAPIEALETGPRTIPICEQSTGALTQRKYESNFGQEFVVAGDPRSG
                     EFVVDAARTDAAPKLYQADDFLIIFDPRAGMRGVIGPNRIRGPIKDKDTDGGYYDAAQ
                     DKDNTDVHFRRYNFTGVTLYHRSAFLNATRAGMTAADARERQEWLRNNSRPFLVHRFG
                     GGLVSGAQSEGEQP"
     gene            complement(1332..2465)
                     /locus_tag="PLU99_01175"
     CDS             complement(1332..2465)
                     /locus_tag="PLU99_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO15694.1"
                     /translation="MSDSTSRSRAYEHGQQCGRGAAFTLVELLVVIVVIGIVMSLLLL
                     VGGRVLDHQKRAATQNTMRLTEMAIEQFKTTDPLRDIYNNPKLRDRSGGTNRILGCTF
                     GPYPPYQLAGDEVSVTQDPGNVRSRVEPREALQPHLDGDKYTLHRRLVLDLGLAPSDD
                     NDAHISIRRSSERPTTDEDYLAYYSYDNRALYCYLAAFTPGVLDQIPDSAKKPLRRAA
                     SASAGSTDTTEMIDPDGGLLDRPGPRAFDVLGIYDAWGVPLDYFVQAKLERKIGPNGT
                     EQWVVTDRVPVLRSRGVPREKAIAADKAAYGGDDTLREDEEGNWIFSQPFPRPAFRSD
                     SDQIDAKTGEIKRTDANVGGWVRVRAGSVPNDDKTWDSYKYIP"
     gene            complement(2474..3655)
                     /locus_tag="PLU99_01180"
     CDS             complement(2474..3655)
                     /locus_tag="PLU99_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15695.1"
                     /translation="MRAEVRSAKSEGRRQRTEPAPGAPGPKLHSPNSKLEAAFTLVEL
                     TIVVAIIAVLVGIMVPAVSRVHMRSKNMSCKTVLSAIDTGLEMFKADGNVGGDYPPSY
                     SDAEDGVGDLRRGQVANPYGQSGGSGGGYSGPGVSLPIAITGSGLLVWGLAGADQLGT
                     PGFKVFRSDNDTQLWSADTHNRAGAQYARDKSGAYAMNEEKGTPVQPRFGPYVDLSKV
                     EITPYDSSVRNFVVPAETEARGQPYGRRYPMFLDSFGYPILYWKADPAGNRIVDMRRT
                     DYTGADRGIYHWEDNGVLLEHPDGPDEFNDEEPLQLTDKRLDFMQALYWDEDWAPDAY
                     DANPTTPTDPPPSKSFADFIMNPKITAKLTPHRPNSYLLISPGYDGEYGTADDITNFN
                     K"
     gene            complement(3688..4965)
                     /locus_tag="PLU99_01185"
     CDS             complement(3688..4965)
                     /locus_tag="PLU99_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO15696.1"
                     /translation="MAVFQYEALNASGQEIRDSLEAPTKEDAVAKVRGLGYFPTKVVE
                     KTDKKRIVARQVSGTRKRKAAGTGFGRVPVKQLTAFTRQLSTLQDAGLPILRSLRILE
                     QQQKPSMLRRVLKEVHDDVEGGSTLSEALTRHPKAFNRLYCNMVAAGEAGGVLDVILQ
                     RLAEFMEKGQRLRRKVIGAMVYPAVVITIAAAIVTFIMVQVVPKFRDIFRDFGATLPA
                     PTEILIATSTWFAKGSKDVPVPGWVVVVVSPLLIFAIFKLIRQAKIGRYVIDTSLMSL
                     PVAGQIASKSSIARFTRTLGTLIAAGVPILEAINITRDTTGNEVFARMLQKVHDSIRE
                     GDTFANPLRASKTVDAIVVNMVDVGEETGELDKMLMKVADNYDEEVDTLVGSLVSLLE
                     PIMVVILGLIVGYIVVALFLPLVQLIQTLQAGS"
     gene            complement(5284..7035)
                     /gene="gspE"
                     /locus_tag="PLU99_01190"
     CDS             complement(5284..7035)
                     /gene="gspE"
                     /locus_tag="PLU99_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOO15697.1"
                     /translation="MATEGMPPISQLKGRPLGRILIKMGKVTRAQVSEALELQKKKRG
                     PLGQLMIEMGFVDEATVNRALAAQVGMDSITIADIDIDKTVIDLIPAQMAQAYRIIPT
                     AYDRDTNTLEVALATPDNFQATDDLKTLMGYNVIARITTPEDMADALNRYYPEEAAES
                     ISDIIGELGDDEEVTRLQGRGESIDLDELRAAAESSPVKKLVNLVLLQAIRDKASDIH
                     FEPFEDEFKMRYRIDGVLFEMVPPPKYVAMAISSRIKVMANLDIAERRLPQDGRIELL
                     VEGRPVDLRVSVLPTMFGESVVLRVLDRAQVNLDLEMLGMREDDLNSMRQLIAKPHGI
                     LVVTGPTGSGKTTTLYSALRELNSPEVKILTTEDPVEYDIDGLIQCQIKQEIGLTFAR
                     CLRSFLRQDPDIILVGEIRDLETAQIAVQASLTGHLVFSTLHTNDAPSSIARLLDLGM
                     EPFLLTATLEAIVAQRLVRKICENCKEEFHPSEQQLLALNITPEHIADRPLFYGRGCD
                     YCNNTGYRGRVGLFEIMLMYEELKELIMRNASTALLRQEARKRGMRTLRESGLLTMYD
                     GITSLEEVVRETLSDEM"
     gene            complement(7241..8119)
                     /locus_tag="PLU99_01195"
     CDS             complement(7241..8119)
                     /locus_tag="PLU99_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO15698.1"
                     /translation="MLAGCVPIAMLEPAEVVAGLSDLILHPDTTCEELRARFGLDLEI
                     VNNPAEAGLSYEEVRIPIDETKTLRAWYMPTEGDLGTVIVSMGDAGSMACYLYTAKLL
                     YSNGWSVVMYDYEGFGGSDGPPTLYALSRDLRAVIDWTRAATGRDQVTLMGMSLGSIP
                     SIALAVQYPEAVNAVVLDSPIALRRQIFRFGFLVGGQSAPLVDRLSSDLLSDDLMSGL
                     HQPLLIFAHGRDFISDPASVELLFERASGPKQLVMFPTLGHSSGQFYATGYYVQYLTD
                     FLMRVWTGYDFAAAGE"
     gene            complement(8723..8995)
                     /locus_tag="PLU99_01200"
     CDS             complement(8723..8995)
                     /locus_tag="PLU99_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15699.1"
                     /translation="MDEAIFQKKLAELVEQIETLPENERERLRELAAETQERHEQIQN
                     SVRSLQESIDFLRVGIKYVLFDLEATRRENAQLRKMLENDQNNGQQ"
     gene            complement(9298..10293)
                     /locus_tag="PLU99_01205"
     CDS             complement(9298..10293)
                     /locus_tag="PLU99_01205"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HOO15700.1"
                     /translation="MSGAASQPQAIYSFRHELIRHDSPALCQGGVFESPTIAAQGEFN
                     EALLSWNVDVEPGTSFCVELRVGRRRENYWTPYLHLGDWGPAAVRPRLVKCDSARVDV
                     DILRSDERFDRAQYRVIAQADGRPAAASIRHVTLCLSERLGPLPGHTLNAAAAAIRPG
                     RLPVPFRSQRAVAPDLAPRICSPTSLAMVMQYRGVNVPTETVAAACYDPAHDIYGNWP
                     RNIQAAYAFGVSGYLARFSDLNAAAELVAAGQPLIISIRFSCPGALRGAPYLTTDGHL
                     LVCCGFDASGDVYVNDPAAATDQEGCTIYDRQELEEAWLRGGGVAYVLLPTEPSR"
     gene            complement(10290..13229)
                     /locus_tag="PLU99_01210"
     CDS             complement(10290..13229)
                     /locus_tag="PLU99_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOO15701.1"
                     /translation="MAALQAVIEELSSGSTCEIRTDRLARALYATDASIYEIFPDAVV
                     FPRSVADVQALVRACARHRVPLTPRGAGTGLTGGCVNRGIVLDLSRYLNRVLEVDPQA
                     RTARVQPGVVLDELNAQLRQHGLHFSPDVATASRATIGGMIGNNSAGAHSIVVGRTCD
                     HVLGLEVVLSDGSLASWGEAARRQPRLPAGDGPSLAVQCERAVAAVASEYADEIAARF
                     PKVFRRNGGYALDRVRTVDGRTNVEQVIVGSEGTLCVTVAATLKLLPLPVCKGLAVIH
                     YDDLVAALRSVPAILAHQPAAIELLDKLILDGTKDNPVMRPRRWFLEGDPAAILIVEL
                     YDDNSAALQRRLEDLAADMRARRIGYACRVITEPPRQADVWDVRKAATGLLLSRPGDV
                     QPHDFIDDCAVEPARLADYIARLNEIMAEEGVEQAGYYAHASVGLLHVRPALNLKTHE
                     GVQKLRRIADRVSLLVREFGGALTGEHGEGIVRSEWIERMFGPALVEAFRRVKTAFDP
                     LGILNPGKIVDPLPMDANLRYGGDFESDQPATVLDFSKYGGMAGQAEMCSGVGDCRKR
                     LVGTLCPSYMATGDETHTARARANALRLALSNRNLLDGLSDPALDEVFDLCLSCKACK
                     TECPTGTDVAKLKTEWLHKRNQRLGVPRRSRLIARSVDLAVWGSRFAPLSNWLLGSKA
                     VRVMMEHLYGLDRRVPPPRFARQTFRAWFGRRNAAADSAAGGRNVAHGRPVGRRTRVV
                     YFADTWTNFYTPQVGVAAVSVLEALGWEVIVPPTVCCGRPLISKGLLDEAARLARANV
                     EILGPFAERGIPILSTEPSCVSALTDELPQLVRTAAAREIAGRVQPLEQFVAAALREQ
                     PELLHRGTAATPAVAPALLFHGHCHQKALTGTADVMHILKLCTPAAAEEINSGCCGMA
                     GSFGHEVEHYEVARAIGEQRLFPAIRARGAADIAVAGFSCREHIAHHTGVGARHWVEY
                     LAEALKP"
     gene            complement(13416..14270)
                     /locus_tag="PLU99_01215"
     CDS             complement(13416..14270)
                     /locus_tag="PLU99_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15702.1"
                     /translation="MRSSTELVRFAAVALFAAAVGLSAGCNPISRVDLGTDLRGSQHG
                     RVYYVGGAGTFGNVGMIDVPKGLRRAGWRGSVEVFKWQSTLGGTVRDQIDHERNREKA
                     RELAREIVSYARRYPGRPVNIVALSAGTGIATWALESLPDGVDVENVVFLASSLSRSY
                     DLTSALRRVKRRVYNFYSPDDPVLRYALPITGSVDREHELLGSPAVAGLLGFELPDVS
                     SEHARMLYDARVQNMPYRKKYREYGYRGNHTDVTSADFIEHVVAPLIVRQRRMASGVP
                     TPDPADSR"
     gene            complement(14572..14967)
                     /locus_tag="PLU99_01220"
     CDS             complement(14572..14967)
                     /locus_tag="PLU99_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOO15703.1"
                     /translation="MEVRMDLAQIVICETRDQQLIVLRERNGPRVLPIVIGLTEALAI
                     DRRVKGVPLQRPMTHDLLANTIESLSAELERIVINDLQEHTFYAKLILRSHGELIEVD
                     SRPSDAIALGIANKVPIYVEEGVLKQVEP"
     gene            15101..>16804
                     /locus_tag="PLU99_01225"
     CDS             15101..>16804
                     /locus_tag="PLU99_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO15704.1"
                     /translation="MLRILITIIIAPVLAVLTWLALGEHAARPDFVVASQEPRTLDPH
                     RVSWIAEIQLANTLFEGLTRLNSDTFQPEPAVAQSWSVDERQTSWTFHLRPDARWSNG
                     EPVTTGDFRFAWLRVLDPAIGAQYAGLMFIIDGAEAYYRSRLPGSAQGELPPETVGIE
                     TPDEHTLRVRLSAPCSYFLDLTSFPTFAPVHPATVERWSYRDGRAMPQTQHLWVRPGR
                     IVCNGAFVLMHWGFKQSIRLERNPHYWDRSTIDVDSIQVHITADPYAALLAYETGSVD
                     LVHGLESSVARVLMLEQQAGRRPDFHIGDRFATSFYRVNCLRPPLDNADFRRALSLAI
                     DRTAICERVLCLGEKPAETYIPPGLAPLMPRRTPDGRTIYYQSPRPLGAGLTNAARVE
                     LAREHLHRYMISAGVEDAAHIRPIELAFGSDPEQRRIAQAIQQMWETALGLKIELRTL
                     EAKVLSSRLRDLDYDLVRSSWFGDYMDPSTFLTMFTTDDGQNFTGWSNPTYDRLIASA
                     AVEADNERRYELLSAAEQLLCNDCPILTVFHRRGNFVLNERFTGLNDHPRDLMPLQRV
                     RR"
     assembly_gap    16805..16904
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16914..17945)
                     /locus_tag="PLU99_01230"
     CDS             complement(16914..17945)
                     /locus_tag="PLU99_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO15705.1"
                     /translation="MQRTHRHDGPDKKHPANRLGLDYRQPPAPRAPEMRILDVHSHVH
                     LSRSAAAFFEAAALYGVERVISMSPMEDARRLQADHADRVGFIAVPQWRGFEESHSFR
                     ERWLADLAAFRELGARVMKFWVAPPLRAQFGLTLDHEYFRPVIHRALDLGYDFMVHVG
                     DPDEWFLPGGRYGDPRRFGTKADQYPQLEWFLERVAPRFVIAAHMGGRVENPEFLNDL
                     LARHSNLYLDTSATKWIVRGVSARPAAVRDVFLAHPDRLLFGSDLVVGDQYDFQHYAS
                     RYWAQRTMWETAYDGESPIEDPDAPAAPRLRGIDLPTQVLRKLYWENAAKLGFAQEAG
                     ALTTGAARR"
     gene            complement(18056..>18711)
                     /locus_tag="PLU99_01235"
     CDS             complement(18056..>18711)
                     /locus_tag="PLU99_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15706.1"
                     /translation="GFSASAAVFGASLNGRGRLFEMNDERVALIVDVDHTIARTDYRA
                     LLFSPLDGKSQPIPGSREVLWKLAEHFNIVYLTGRPRVMQAKTRAWLAENDYPPGPLV
                     VAPAIRKAADNGKFKLQVLSHLRTKWPNLLIGVGDRPSDARAYGANEMLALVIAPHEI
                     EEIGTHALLMPDWKAVNTFFEANHDALTDPTTVRELVAGRRPVLFTMTPYSAVADSD"
     assembly_gap    18712..18721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18722..19031)
                     /locus_tag="PLU99_01240"
     CDS             complement(<18722..19031)
                     /locus_tag="PLU99_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15707.1"
                     /translation="MRGRGGFRGRRAVRLLLAVGAAMVGTGGCGRPVLTLEDGVAVDD
                     WRQVELTAHVEHQLFGLLRTSVSNVPVRFFSGDNQLGEARTDEDGSATIRCDVGELGD
                     S"
     gene            19133..20263
                     /locus_tag="PLU99_01245"
     CDS             19133..20263
                     /locus_tag="PLU99_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO15708.1"
                     /translation="MLRGFRHTLFLLVQSGIVISAALAFLIYPEAARAIAICMAGGMV
                     AVWLSERLARHWLRATLGRLRRLAEDIGQGRPTGALAVRKGDDLYKLVSAVNLLAARV
                     EEITREEKRLQEQLRKTEQLALLGELAASVAHEINNPLDGIQSCTRILRRSLHDPART
                     EQMLALIDSGLARIGLIVRRLLTLAREHVIRTVEVRARDVLESAIAAVANKLESGGIQ
                     VARHYDAGDDRILADAQLLEQVFVNLFLNAADAMSKGGTLTLTTRLEHPAEIVPEAAP
                     QILSVEVRDTGHGIPANLLPRIFEPFFTTKTAGKGTGLGLPIAARIVDAHRGTISVKP
                     IKGGGTVFTVRLPAFRPDHKRADATPSSPALLSAEPVSSTVE"
     gene            20429..21034
                     /locus_tag="PLU99_01250"
     CDS             20429..21034
                     /locus_tag="PLU99_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOO15709.1"
                     /translation="MEWECSTEGRPSDAELVRQTCAGQRSAFDELIERYQRRATSVAY
                     RLLGDLHDALEVCQEAFVRAYQNLDSLEDQQRFGSWLLRIVTNLSLNFRRSRAAIARK
                     AAFDDCLNDGETARDEQIAAPDYSDERPEARSAAAELSLLVQKAIADLPDQQKTALVL
                     FSIEQLPQKEVASIMGCSVEAVKWHVFQARKKMREALAPYL"
     gene            21051..21866
                     /locus_tag="PLU99_01255"
     CDS             21051..21866
                     /locus_tag="PLU99_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15710.1"
                     /translation="MDRLAFEQLISTWLDDRENSDLCRQIDAACADDPACRRLVAEYE
                     KLDSLVRHASDQPLAVDWPAFKAVVSSEIAAATQPDTAAKLDRLLQTALPAVEPQVNW
                     QRFGRRVSQAIDQSVATRRVLRLSRWRAAALGGLAAAAALLLWFGLPPEQAPVAQPSA
                     PPAGVVEQPLVATEQTLLPAAPEPSARPPKVTIALAEPHEMVAPPTKAAGALAGQRRA
                     AMVRVHVHEEPGQPPALARTASRQGQRTSEPEVFFMLEPPDRTVLASAAGFGY"
     gene            21881..>22724
                     /locus_tag="PLU99_01260"
     CDS             21881..>22724
                     /locus_tag="PLU99_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15711.1"
                     /translation="MNRTRRFCWLSWMAPALCLALNGPLAQAVDLRDLVEEVLDQPAE
                     GVEIGPLPINQALEQLGERTGLRFELDAQAIACMPYGENTRISLAFSGLTVRQGLRQV
                     FDGLGLAFEVVDDEVRVVRGPVLERLGRRLAIEEVRTLQQLATHKWSDLDPKWRQLQF
                     RGVKDANAAATLENAIAGVIAGNAVRQLEAATQSLNWLWTPLDTSILVYSQAEDVWNR
                     LQRRISLNYHGLRLDDLLIDLGERVGVTIMFESGVLERIQADDRAVDLVHQNTTVLQT
                     LERIC"
     assembly_gap    22725..22824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22825..23142
                     /locus_tag="PLU99_01265"
     CDS             <22825..23142
                     /locus_tag="PLU99_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15712.1"
                     /translation="VDLVHQNTTVLQTLERICGATHTCFEVQDYGVLIAMPADSERPG
                     LRTPPAPPAVECRVVAILRVPVGDDGTTVDFPFYEHNVPPEFRRLLEQKLPVVIDELR
                     KGS"
     gene            complement(23178..24464)
                     /locus_tag="PLU99_01270"
     CDS             complement(23178..24464)
                     /locus_tag="PLU99_01270"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO15713.1"
                     /translation="MKEATPVASIDMIFGESPALQAVMHNFMQAAKVLELEHNLAVRL
                     ARPDKVIMVSVPVQMDDGRIRVFTGFRVQHNHALGPYKGGIRYHPDVDVGEVTALATQ
                     MTWKNGLMNLPLGGAKGAVRVDPRQLSENERQQLTRRYTAEIAPFIGPEFDIPAPDMG
                     TDELTMAWIMDTYSQHVGKFTPHVVTGKPVDVGGSVLRKEATGRGVVYCIEEAAREIG
                     LKLDGATAVLHGFGNVGVHAATELARRNVRLTAVADITGGYVCERGLDVERLLAHVRR
                     HGDLQDFPDAERVPAVDVPTIPCDILVPAATGGVITRTNAPRIKCRILAEGANTPTLP
                     EADPILENNGVFVIPDTICNAGGVIVSYFEWVQGGINFFWSAAEIDTRLSDLIRAGYH
                     RAREFAREHQTSTRTAALCVGIARVASSMRLRGLYA"
     gene            24751..25344
                     /locus_tag="PLU99_01275"
     CDS             24751..25344
                     /locus_tag="PLU99_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15714.1"
                     /translation="MTTRATALPLLAALIALISSAGCSHIIKPAYYELRGAKGTIYWV
                     EEPPVSSLDEFRSVYFEPLSTTLGARLCPPELLEEYNRTARVYEAKLENYPGGSPTLS
                     VGGDVVYFQNKGLLSAALLLMRFKLTADGRQVGDGVLRVESKSFREGDIGDLAETAVE
                     TLSKYLDEEKMPRDDDPERRRARRPFGWFNKLTRAPG"
     gene            26174..27790
                     /locus_tag="PLU99_01280"
     CDS             26174..27790
                     /locus_tag="PLU99_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO15715.1"
                     /translation="MTQALSDLSVLAGRPADREPAGEDPEAPRSGAGVPVPKARWKTR
                     VLLPGAILCITAATLLMTAWRALWPATPVRVAPVVLKTGVQPAGKVIVQAPGWVEADP
                     FPTAVTALADGVVAEVLVLEGQRVEPNQVVARLIADEARLALAKAESALHAAAADVAA
                     AQAELKAAQREWDNPIELTRKLATSQAELAQRQAELARWPAELEAATALAEYKQAEYL
                     RVKPLHGSGSVSNIEVIRAEKEYENQKALAQAVRDMKPILEAQIAHLEAEVTAARENL
                     RLRIGETRRLEAAQANLERMQAARLVAEAARDEAALRLERMEVRAPVGGIVMNRLVEP
                     GSKLMLGGTEMDSALVMRLYDPQKLQVRVDVPLTDAGRVGVGQRAEVVVDVLPDRVFQ
                     GRITRIVNEADIQKNTLQVKVAIADPAPEIKPEMLARARFFSDPETASDTPGQRLFVP
                     QSAIVRHAGGHTMVWVADQARNMALPRTVTVASTTADGWVEIESGVQPGDRVIVDPPQ
                     DLDDGARIRIAGEIGGDSFSPTKGIGHGAH"
     gene            27777..28472
                     /locus_tag="PLU99_01285"
     CDS             27777..28472
                     /locus_tag="PLU99_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO15716.1"
                     /translation="MALIECRSLTRSYRKGDIVITPLDGLNLDVEEGDFLALMGPSGS
                     GKTTLLNLIAGIDRPTSGELYVDGTDISQLSRAQLASWRSKYIGYIFQLYNLVPTLTA
                     YENIELPLLLHKLSRRQRHAKVALALELVNLADRHDHFPRQLSGGQEQRVAIARAIVT
                     NPKIIVADEPTGDLDSHAAAAIMDLLVRLNRELGKTLLMVTHDARCAAYARHTLHLEK
                     GALVEAPGATVAA"
     gene            28485..29639
                     /locus_tag="PLU99_01290"
     CDS             28485..29639
                     /locus_tag="PLU99_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15717.1"
                     /translation="MLAPKFMPFVLKQVVRHRVRSLLTMCGVAVAMFLFTAVQSMQAG
                     VEAATTANATDNTLVVYRKDRYCPFASRLPQSYFEQIKRLPGVESVVPIKIVVNNCRT
                     SLDVVTFRGVPEEPFIDSYAPRLRLLSGSLDDWKRRSDAVLLGETLARRRGLNVGDRF
                     EAAGITVYVAGVVQSDEPQDENVGYTHLDFLQFATGSRAGGIVTQFNVRVSDPSQLEP
                     VAAAIDELFAADQEPTRTSPEKAFVARAAADVIEIAGFMKWLGLGCLLAVLALVANAI
                     VLSVQDRVREHAVLQTLGFRGNLIARLIIGEGLVISALGGLVGCALAALILSRGGFAL
                     SVDALSIPIKSGLPVFLWSMLIACTLGILAGLVPAWQASRREIVTCFRAV"
     gene            29712..30881
                     /locus_tag="PLU99_01295"
     CDS             29712..30881
                     /locus_tag="PLU99_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15718.1"
                     /translation="MKLLPFEYAVRNLGRSPLRLAASLIGAALVVALVLAAGSFVRGM
                     ERTMSVSGSSKNVILLGAGSEESIERSQIGLNVAGLVTGSVPGIRTNLGVPYVSPEVH
                     MALLVRTGREAEQELNAVLRGITNTAYLVHPQVRVVEGRPPLPGEYELMVGSLAATRM
                     GVPSEALAVGRSLWFDNHAWTITGRFEAPHTVMDAEIWVPLTDLQVATRRESTISCVI
                     LTLDDAEYEDVAAFAAQRLDLEIRAVRESDYYASLLKFYRPVRGLVWATAILIALGGL
                     FGGLNTMYAAFASRVRELGALQTLGFSRTAIGVSFLQESLLASSAGSLAAALICVPLL
                     NRVAVKFSMGAFALVLDAPVLIGGLAAGAALGIIGIIPPLWRCLRLPIVESLRTA"
     gene            30897..>31315
                     /locus_tag="PLU99_01300"
     CDS             30897..>31315
                     /locus_tag="PLU99_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15719.1"
                     /translation="MRTLITSTLLLNLAILAGCDQSAQSPPPAKPAASAASTPAAPDS
                     ALPPGLWLSAEPADAKPVGEIKKNAAAGQDVVLFGRIGGSRDPFVSGRAMFTLVDRSV
                     PSCTDRHGDGCPTPWDYCCEPRENLLANTVTIQVLGA"
BASE COUNT         5650 a         9984 c         9765 g         5706 t
ORIGIN      
        1 ggcctggccg gtggcgcggt cgatcatgtc gcgttcatat tgcaagccaa ccggcagcgc
       61 ggcgccgatt accagcaggc cgaagcccag gatgaccaac gcgatcatca tctcggcgag
      121 gctgaaagca gttttgcgtg caacccctgc tctcatggct gctccccctc tgactgcgcg
      181 ccgctgacca ggccgccgcc gaagcggtgg accaggaacg ggcggctgtt gttgcggagc
      241 cactcctggc gctccctggc atcggcagcc gtcatgccgg cacgggtcgc attgaggaac
      301 gcgctgcggt gatacagcgt cacgccggtg aagttatacc ggcggaaatg gacgtcagta
      361 ttgtccttgt cctgagcggc gtcgtaatag ccgccgtcag tgtctttgtc tttaatcgga
      421 cccctgatcc ggttcggccc gatgacgccg cgcatgcctg cacgcgggtc aaagatgatc
      481 aggaagtcgt cggcctgata cagcttcggc gccgcgtccg tgcgggccgc gtccacgacg
      541 aactcgccgg agcgcgggtc cccggcaacc acgaactctt gaccgaagtt cgactcgtac
      601 ttgcgctggg ttaaggcgcc ggtgctctgc tcgcatatcg gaatcgtccg cggcccggtt
      661 tccaatgctt cgattggcgc gacccagacg tcttccggca atatgaccga tgacgtgccc
      721 gggcggcgct ggaaatactc gccgaaaacg acctggccgg gatgaaccgg gtcggccgcg
      781 ctcgtcggcg tgaagctgta tgtgacaatg tgctgccggc cggtcgtgcc ggattcgctg
      841 gttgaggggt cgttgtccca tttaccgggc acgaagcgca cggccgtcat gttcaggtcg
      901 gccaaggccg cggtgtaggc gtgcgtcagg gcgccgttgg tgatctggac ggcggcgctg
      961 aagcgtgact cggccgcgat ggcattcagt ccggggatgg cgatggccaa tatgaggacg
     1021 atgaccgaca tgacgacgat cagttcgacg atagtgaagg ccggggtcga acagagccgg
     1081 cggtagccgg cggcgacgga ggaccggcgc ggaggaagcg caggccacaa ggcaaccaag
     1141 ccgcgacgcg cggcagtccc gcggaccacc tgcgtgtctg cgtgcctacg taccttcatg
     1201 cctggttgcg cgcctgcgcg ccttaaactg cttcgatatt cgcgtccctt cggtcgcaag
     1261 ccaacgcact ccgtaagtct ttcgctgtca gcattctacg tttccgccga atgtctaaag
     1321 ctgacgcccc ctcagggaat atatttgtaa ctgtcccatg ttttgtcgtc gttgggcacg
     1381 ctgccggcgc ggacgcgcac ccagccaccg acgtttgcgt ctgtacgctt aatttcgccc
     1441 gtcttggcgt cgatctggtc agaatcgctt ctgaacgccg gccgcgggaa tggctgactg
     1501 aagatccaat tcccctcttc gtcctccctc agcgtgtcgt cgccaccgta ggcagccttg
     1561 tcggcggcta tggccttttc gcgcggaaca ccgcgggagc gcaggaccgg cacgcggtcc
     1621 gttacaaccc actgttccgt gccattgggg ccaatcttgc gttcaagttt tgcttgcaca
     1681 aaatagtcca atggcacgcc ccaggcatcg tagatgccca gtacgtcgaa agcccgtggg
     1741 cccggcctat cgagcaggcc gccgtcggga tcgatcattt cagttgtatc cgtgctaccg
     1801 gcagaggcgc tcgctgcgcg gcgcaatggc ttcttggccg aatccggtat ctgatccaag
     1861 acgccgggtg tgaacgcggc taggtagcag tacaacgccc ggttatcgta gctgtagtaa
     1921 gccaggtagt cttcgtcggt agtcggcctc tcgctggaac gcctaattga aatgtgggcg
     1981 tcgttgtcgt cggacggggc aagaccaagg tccagcacaa gccggcggtg cagcgtgtat
     2041 ttgtctccgt ccaggtgagg ttggagcgct tcccgcggct cgactctcga tcgcacattg
     2101 ccggggtctt gcgttaccga aacctcatcg cccgcaagct gatacggcgg atacgggcca
     2161 aacgtgcagc ccaggattcg gtttgtcccg cctgaccggt cgcgcagctt tggattgttg
     2221 tagatatcgc gcagcgggtc agtagtcttg aactgctcga tggccatctc ggtcaggcgc
     2281 atcgtattct gcgttgcggc ccgcttctgg tgatcgagca cgcgcccgcc caccagcagc
     2341 agcagactca tgacgatgcc tatgacgaca atgacgacca gcagttccac cagcgtgaat
     2401 gccgcgccgc ggccgcactg ttgtccatgt tcgtaggccc ggcttctgct ggtgctgtca
     2461 gacatcccgc acgctatttg ttgaagttgg tgatatcgtc ggcggtgccg tattcgccgt
     2521 cgtagccggg gctgatcagc agatacgaat tcggtctgtg gggcgtcagc ttggcggtta
     2581 tcttcgggtt catgatgaag tccgcaaagc tcttggatgg cggcggatcc gtgggcgtgg
     2641 tgggattagc gtcgtaagca tccggcgccc aatcctcgtc ccagtacagt gcctgcatga
     2701 aatccagacg cttgtcggtg agttgcagcg gctcttcgtc gttgaactcg tccggcccgt
     2761 ccgggtgttc cagcaataca ccattatctt cccaatggta aatgccgcga tcagctccag
     2821 tataatccgt acgccgcata tcaacgatgc ggttgccggc cggatcagct ttccagtaca
     2881 ggatcgggta accgaacgaa tccaggaaca tcgggtaacg ccgcccgtat ggctggccac
     2941 gcgcctccgt ctcggccggc accacaaagt tgcggacgct ggaatcgtaa ggcgttatct
     3001 caaccttgct cagatccaca tacgggccga aacgcggctg aacgggcgtc cccttttcct
     3061 cgttcatggc gtaagcaccg ctcttgtccc gcgcgtactg ggcgccagcc cgattgtgcg
     3121 tgtcggccga ccagagctgg gtgtcgttat cgctgcgaaa cactttgaaa cccggcgtgc
     3181 cgagctggtc ggcgccggcc agcccccaga ccaataagcc actgccggtg atcgcgatgg
     3241 gcaacgagac gcccgggccg ctgtagccgc cgcccgagcc gcctgactgc ccataggggt
     3301 tggccacctg cccacgccgc aggtctccca ccccgtcctc cgcatcagag tacgacggcg
     3361 gataatcgcc ccccacgttc ccatccgcct tgaacatttc caggccggta tcgatggccg
     3421 acagcacggt cttgcagctc atgttcttgc tgcgcatgtg cactcggctg acggccggaa
     3481 ccatgatgcc cacgagcacg gcgatgatgg ctacgacgat cgtgagttcg acgagcgtga
     3541 aagcagcttc gagtttcgag tttggactat gaagtttcgg cccgggcgcg ccgggagcgg
     3601 gctccgtcct ctgccttctg ccttcgctct tcgcacttct gacttctgct cgcatgcagc
     3661 accttttcga cccgcgtccg ccacgcatca ggagcctgcc tgcagggttt gaatcaactg
     3721 taccagcggc aggaacagcg ccaccacaat ataaccgacg atcagcccca ggataaccac
     3781 catgattggc tcgagcaggc tgaccagcga gccgaccagc gtatcgactt cctcgtcgta
     3841 gttgtcggcc accttcatca gcatcttgtc gagttcgccg gtttcctccc ccacgtccac
     3901 catgttcacc acgatggcat ccaccgtctt cgaggcgcgc agcgggttgg cgaacgtgtc
     3961 gccttcgcga atcgaatcgt gcaccttctg cagcatccgc gcgaaaacct cgttgcccgt
     4021 cgtatcgcgc gtgatgttga tcgcctccag aatcggcacg cccgccgcta tcaacgtgcc
     4081 cagcgtgcgc gtgaaacgcg cgatactgga tttcgaagcg atctgcccgg ccaccggcag
     4141 gctcatcagg ctggtgtcga tgacgtaccg gccgatcttg gcttggcgga taagcttgaa
     4201 aatggcaaag attagcagcg gcgaaaccac caccacgacc cagcccggga cgggcacgtc
     4261 tttcgagccc ttggcgaacc aggtagacgt ggcgatcagg atttcagtag gcgccggcag
     4321 cgtggccccg aaatcccgga agatgtcgcg gaacttcgga acgacctgaa ccatgatgaa
     4381 ggtcacgatg gccgcggcga tcgtgatgac gacggccggg taaaccatgg ctccgatgac
     4441 cttgcggcgc aggcgctggc ctttctccat gaactcggcc aagcgctgga gaatgacgtc
     4501 gagcacgccg cccgcctcgc cggccgccac catgttgcaa tagaggcggt tgaaagcttt
     4561 ggggtggcgt gtgagggctt ctgaaagggt cgagccgcct tcgacgtcgt cgtgtacttc
     4621 tttcaggacc ctgcgcagca tgctcggctt ctgctgttgc tcgagaatgc ggaggctgcg
     4681 caagattggc agaccggcgt cttgcagcgt cgaaagttgc cgcgtgaagg ccgtgagctg
     4741 cttgactggg acgcgcccaa aaccggttcc ggcggccttc cttttgcgcg tgccggaaac
     4801 ctgccgcgcg acgatacgct tcttgtcagt cttctcaacg accttggtcg ggaaataccc
     4861 cagaccgcgg accttggcga ccgcgtcttc tttggttggg gcctcgagcg aatccctgat
     4921 ctcctggccc gaggcattca acgcttcata ttgaaaaacc gccatcgagg cactccgagg
     4981 gcacccaagc tccacgctct gcacactcgc tgctgccggc cgcccacgcg ggcggcccac
     5041 cttcagccgt ctcgaactgc actggcatta tgaatgccac caagtggccg gagaatgcgg
     5101 gttcattaca gctttataag acggacggat aggcgtcagg tgcatggaat tcgcgacggc
     5161 atttcgggac gatgttgaga tcggctgata agaaaagagc cggcgcaaac cgccggcgcg
     5221 ccattcgctg agcgggcccc ccgctgcacc tcttgatccc tagatccctt gatccctaga
     5281 tccctacatc tcgtcgctca gcgtttcgcg gacgacttct tcgagtgagg tgatcccgtc
     5341 atacatcgtg agcaagcccg actcgcgcag ggtgcgcatg ccacgcttgc gcgcttcctg
     5401 ccgcaacagt gcggtcgatg cattccgcat gatcaattct ttgagctcct cgtacatgag
     5461 catgatctcg aacagcccga cgcgcccgcg atagccggtg ttgttgcagt agtcgcagcc
     5521 gcggccgtag aagagtgggc gatcggcaat gtgctccggg gtgatgttca gggcgaggag
     5581 ttgctgttca gagggatgga attcctcctt gcagttttcg cagatttttc ggacgaggcg
     5641 ctgggcgacg atggcttcca gcgtggcggt caggaggaat ggctccattc cgaggtcgag
     5701 caggcgtgcg atagagcttg gggcgtcgtt ggtgtgtagc gtggagaaga cgaggtggcc
     5761 cgtaagtgac gcctgcaccg cgatttgcgc ggtttccagg tcgcggatct caccgaccag
     5821 aatgatgtcg gggtcctgac gcaggaagct gcgcaggcaa cgggcgaacg tcagaccaat
     5881 ctcctgcttg atctggcatt gaatcagacc gtcgatgtcg tattcgaccg ggtcttcggt
     5941 cgttaagatc ttgacttcag gagagttaag ctcgcgcagc gcggagtaca gggtggtggt
     6001 tttgcccgag ccggtaggtc cggtaacgac aaggatgccg tgcggcttgg cgatgagctg
     6061 ccgcatcgaa ttcaggtcgt cctcgcgcat accaagcatt tcgaggtcca gattgacctg
     6121 tgcccggtcc agcacacgca gtaccacgct ctcgccaaac atcgtcggaa gaacgctgac
     6181 gcgcaggtcg accggccggc cttcgacgag cagttcgatg cggccgtctt gcggcaggcg
     6241 gcgctcggca atgtccaggt tggccatgac cttaatgcgg gaggagatgg ccatggcgac
     6301 gtacttggga ggcggcacca tttcgaagag gacgccgtcg atgcggtagc gcatcttgaa
     6361 ttcgtcctca aagggctcga agtggatgtc ggaggccttg tcgcgaatgg cctggaggag
     6421 gaccaggttc acgagcttct tgacggggct gctttcggct gcggcgcgaa gttcgtcgag
     6481 gtcgatgctt tcgccgcgtc cctgaaggcg tgtgacttcc tcatcgtcgc cgagctcgcc
     6541 gatgatgtcg ctgatgcttt cggcggcttc ttcgggatag taacggttga gggcgtcggc
     6601 catgtcctcg ggcgtggtga tgcgcgcgat gacgttgtag cccatgaggg tttttaaatc
     6661 gtcggtggcc tggaaattat caggagtcgc cagggctact tcgagcgtgt tcgtgtcgcg
     6721 gtcgtaggcg gtcgggatga tgcggtaggc ctgcgccatt tgggccggga tgaggtcgat
     6781 caccgttttg tcgatgtcga tgtcggcaat ggtgatggag tccatgccga cttgagcagc
     6841 gagggcgcgg ttgacggtgg cctcgtcgac gaagcccatt tcgatcatga gctgcccaag
     6901 cgggccgcgc tttttctttt gcagctcaag ggcttcggag acctgggcac gggtaacctt
     6961 gcccatcttg atcaggattc gcccgagcgg acgaccctta agctgtgaaa ttgggggcat
     7021 cccttcagtc gccattcagg caaactccct acgtcgttca ggcaaagtcc gcgaggtatc
     7081 cgctccggac gcggtggtaa gccgccgacc ggctcagggg ctcgaacgcc ggtcgtgcgc
     7141 gaaattatac cgcgggacgg gaagggagga acccggctcc gggtgagagt tgtgcccagc
     7201 ccgatttgag gccggagttt tggctcagcc cggtcttctg ctactccccc gcggctgcga
     7261 aatcgtaccc ggtccagacg cgcatcagga aatccgtcag gtactgcaca tagtagccgg
     7321 tcgcgtagaa ctgtccgctg gaatgcccca gcgtcggaaa catgacaagt tgcttcggcc
     7381 cgcttgcgcg ttcgaaaagc aactccaccg acgcggggtc ggatatgaag tcgcggccat
     7441 gtgcgaagat caacagcggc tggtgcaaac cgctcatcag gtcgtccgac agcaaatctg
     7501 aagagagccg gtcgaccagc ggcgcactct gcccgcctac tagaaatccg aaccggaaaa
     7561 tctgcctccg cagcgcgatg ggtgaatcca ggaccacggc gttgaccgct tcggggtatt
     7621 ggaccgccag ggcgattgac ggaatcgacc cgagcgacat gcccatcaac gtgacctggt
     7681 cgcggcctgt tgcagcgcgg gtccaatcaa tgacggcccg caaatcgcga ctgagcgcgt
     7741 agagcgtggg cgggccgtcg ctgccgccga agccttcgta gtcgtacatc acgaccgacc
     7801 agccgttcga gtacagcagc ttcgcggtgt acaggtagca ggccatgctt cccgcgtcgc
     7861 ccatcgagac gattacggtg cccaggtccc cctcggtcgg catgtaccag gcgcgcaggg
     7921 ttttcgtttc atcaatcgga atccggacct cctcgtacga caggccggcc tccgcgggat
     7981 tgttgacgat ctccaggtcg aggccgaaac gggcgcgcag ttcctcgcag gtcgtatccg
     8041 gatgaagaat caggtcggac aaaccggcga cgacttccgc cggctccagc atggcaatcg
     8101 gcacgcaacc cgcaagcatg caaagactta cagcaatggc actcaaacgg cagaaacgac
     8161 ccgctgtgat catcttctcc gtccctcttg gccagggccg cgctgacgag ctggctagtt
     8221 caattatacg ggcggccggc caatctccag ccttggcctg tatcggctcg ctgggagagt
     8281 tcgtttgccg ctgattttct gagaatattc gaaagattgg cgcaaattga tctacgtgac
     8341 tctcattacc gggcattagt aggcgtcgga aaagagtgct tttggtccgg cgagggtcga
     8401 gatttccgga gtgttgcctt gagtaactga ccggtagtcc gtcaagaaaa gggcggcgac
     8461 gccgcgccct gggcgcaaca gaagcccggc ggcggaccgc cgggctcgat gcgtgcaagg
     8521 cacggcagtg atgaggaccg tgcaggtgaa cggaacagaa gctcgggttt gtgtctcgct
     8581 tagtggccgc caaccggtta gaccaacggg ctgcggggtc gggtaagcct cctcctttgg
     8641 cccaaaagac cggccgcggg tcgcagcggc ctagcggagg ccagccgccg gcgacccgcg
     8701 cgccggtcct tcttgaaact cgctactgtt ggccgttgtt ctgatcgttt tccagcatct
     8761 tccgcaactg ggcgttttca cgccgggtgg cctccaggtc gaagagcacg tacttgatgc
     8821 cgacgcgcag aaaatcgatg ctttcctgca ggctgcgcac gctattctga atttgctcgt
     8881 ggcgttcctg ggtttcggcg gccagctcgc ggagccgctc gcgctcgttc tccggcaacg
     8941 tttcaatctg ctctaccagc tcggccagtt tcttctggaa tatagcctcg tccactcttc
     9001 ttcctcctca gaaaggcacc gggctgtggg tccagccgtt acggcccgac ggagctgcgt
     9061 ccgcgtacat tttgccgatc tcccggctct ccgccgcatt ctaggcacgc aagaaaggcg
     9121 ttcgttgcag cttcgtgagt gcttttgtcc aaatttgcag cgtaggcccc cctattgcag
     9181 cgcaaaattg ctaaaggggg tttttatggg agcgtgaccg ctctcagcag ctcagacccc
     9241 ggtagcgccg cttagtgccc gatattcggc ggacctcgat gctccggcac gctgcattca
     9301 acgcgacggc tcagtcggca gcagcacgta agccacgccg ccgccccgca accacgcttc
     9361 ctccagctcc tgccggtcat agatcgtgca gccctcttgg tccgtcgcgg ccgcgggatc
     9421 gttgacgtac acatcaccgc tcgcatcgaa tccgcagcac acgagcaggt ggccgtccgt
     9481 agtgagatat ggcgctccgc gaagggcgcc gggacaagag aagcgaattg agattatcag
     9541 cggctggccg gcagccacca gttctgccgc cgcgttcaag tccgagaagc gggccagata
     9601 gccggagacg ccgaaagcgt acgcggcctg gatgttgcgg ggccagttgc catagatgtc
     9661 gtgagccggg tcatagcagg ccgcagcgac ggtttcggtg ggcacgttga cgccacggta
     9721 ctgcatcacc atcgccagcg aggttgggct gcaaattcgc ggcgccaggt ccggcgcgac
     9781 ggctcgctga ctgcggaacg gcacgggcaa gcggcccggt ctgatcgcgg cagccgcagc
     9841 attcagcgtg tgaccaggca gagggcccaa tcgctcgcta aggcataatg tgacatgccg
     9901 aatagaggcc gcggcaggcc ggccgtccgc ctgcgcgata acgcggtact gcgcccggtc
     9961 gaaccgctcg tcagaacgga gtatgtcgac atcgacgcgg gcggagtcgc acttcacgag
    10021 gcgcgggcgg actgctgcgg gaccccagtc gccaagatgc aagtaaggcg tccagtaatt
    10081 ctcgcgtcgc cgtccgacgc gcagttcaac gcagaagctc gtgccgggct cgacatccac
    10141 gttccatgaa agaagcgctt cattgaattc gccctgcgcg gcgatggtcg ggctttcgaa
    10201 gacgccgcct tggcacagcg caggcgagtc gtgccggatc agctcgtggc gaaaagagta
    10261 gatcgcctgc ggttgcgacg cggccccact catggcttca acgcctcagc caggtactcg
    10321 acccagtgcc gcgccccaac gcccgtgtga tgggcgatgt gctcgcggca ggaaaaaccc
    10381 gccaccgcga tgtcggccgc tcctcgcgca cggatggccg gaaagagccg ctgttcgcca
    10441 atggcccgcg cgacctcgta gtgctcgacc tcgtggccaa aggagccggc catgccgcaa
    10501 cagccgctgt tgatttcctc ggccgccgcg ggcgtgcaga gcttcaggat gtgcattaca
    10561 tcggcggtgc cggtcagggc tttctggtgg cagtgtccgt gaaacagcaa ggctggcgcg
    10621 acagccggcg tcgcggccgt gccgcggtgc agcagttccg gctgctcgcg caaggccgcg
    10681 gcaacaaact gctccaacgg ctgaacccgg ccggcgattt cccgcgccgc cgccgttcgt
    10741 acaagttgcg gcaattcgtc ggtcagggct gaaacgcaac tcggttcggt actaaggatc
    10801 ggaataccgc gttcagcaaa cggccccagg atttccacgt ttgcacgagc cagccgcgcg
    10861 gcctcgtcga gcagtccctt gctgatcaat gggcgcccac aacagactgt cggcggaacg
    10921 ataacttccc agccgagggc ttccaatacg cttacggccg cgacgccaac ttgcggggtg
    10981 tagaagttcg tccaagtgtc ggcgaagtag acgacacgcg tacggcggcc gacgggacgc
    11041 ccgtgcgcta cgtttcggcc gcccgctgcg ctatccgccg ccgcgtttcg cctgccaaac
    11101 caggcgcgaa aggtctgccg cgcgaagcgc ggcgggggaa cgcggcggtc caagccataa
    11161 aggtgctcca tcatgacgcg caccgctttg gagccgagca gccagttgct cagcggggcg
    11221 aaacggctgc cccagacggc caggtcgacc gagcgggcga tcagacggct gcggcgcggc
    11281 actcccagcc gctgattgcg cttgtgcagc cattcggtct tgagcttggc gacgtccgta
    11341 ccggtcggac actcggtctt gcaggccttg cagctcaggc acaaatcaaa aacttcatcc
    11401 aacgccggat cggacaagcc gtcgagcagg ttacggttgg acagggccag acgcagcgcg
    11461 ttggcccggg cccgtgcggt gtgcgtctca tcgccggttg ccatgtacga cgggcagagc
    11521 gttccgacca aacgtttgcg gcaatccccg acaccgctgc acatctcagc ctgcccggcc
    11581 atgccgccgt acttggagaa gtccagaacg gtcgccggct ggtcggattc gaaatcaccc
    11641 ccgtaccgta gattggcgtc catcggcagc ggatcaacga ttttaccggg gttcaaaatg
    11701 ccgagcgggt cgaacgcggt cttgaccctg cggaatgcct cgaccagcgc gggtccgaac
    11761 atccgctcaa tccactccga gcgaacgatg ccttcgccgt gctcgccggt cagggcgccg
    11821 ccgaactcgc gcacgagcaa gctgacgcga tccgcaatgc gccgcaactt ctgcacgcct
    11881 tcgtgcgtct tgaggttaag cgccgggcgc acgtgcagca ggccgacgct ggcgtgcgcg
    11941 tagtagccgg cctgctccac cccctcctct gccataatct cattgaggcg cgcgatgtaa
    12001 tcagccagcc gcgccggttc aaccgcgcaa tcgtcgatga aatcgtgcgg ctgcacgtcg
    12061 cccgggcgcg acagcagcag gccggtggcg gccttgcgca cgtcccacac atcggcctgg
    12121 cgtggcggtt cggtgatcac acgacaggcg tagccgatgc ggcgagcccg catgtcggcg
    12181 gcaaggtcct ccagccggcg ttgcagggcg gccgagttgt cgtcgtaaag ctcgacgatc
    12241 aggatcgccg cgggatcacc ttccaggaac cagcgccgcg gccgcatgac cgggttgtcc
    12301 ttcgtgccgt ccagaatgag cttgtcgagc agctcgatgg cggccggctg atgggccagg
    12361 atggcgggca ccgagcgcaa agcggcgacc aggtcatcgt aatgaatgac cgccaggcct
    12421 ttgcacaccg gcagcggcaa caacttcagc gtggcggcga cggtcacgca caacgtgccc
    12481 tcgctgccca cgatcacctg ctcgacgttg gtccggccat ctacagtgcg cacgcgatcc
    12541 agcgcgtaac cgccgttgcg gcgaaaaacc ttgggaaaac gcgccgcgat ctcgtcggca
    12601 tattcagacg ccacagccgc gaccgcgcgc tcgcattgaa ccgcaagcga gggaccatcg
    12661 ccggcgggaa ggcgaggctg ccggcgagcc gcttcgcccc aagacgccag cgagccatcg
    12721 gacagaacca cctccagccc gagcacatgg tcgcaagtcc gccccaccac aatcgaatgc
    12781 gccccggccg agttgttgcc gatcatgccc ccgatggtgg cccggctggc ggtggcgaca
    12841 tcgggcgaga aatgcaggcc atgctgccgg agctgggcgt taagctcatc cagcaccacg
    12901 ccgggctgga cgcgtgcggt gcgggcctgc ggatcgactt cgagcacgcg gttcaaatag
    12961 cgggataggt ccagaactat gccgcgattg acacacccgc cggtcagccc ggtccccgcg
    13021 ccgcgcggcg tcagcggcac acgatgccgg gcacaagccc gcaccagcgc ctgcacgtcg
    13081 gccacgctgc gcggaaacac cacggcgtcc ggaaagatct catagatgct ggcgtcggtg
    13141 gcgtacaggg cccgcgccaa ccggtcagtg cgaatttcgc aggtgcttcc gctgctgagc
    13201 tcttcgatga ccgcctggag tgcggccatc ccggcgcgcg atgagtgcgc ctgcggttcg
    13261 gcttgcgtac agctatgtga atcagagcgc acgccgcgcg tactccgggc atttggccac
    13321 ggccatctta atgcgctggc cggcgcggcg taaggatcag cgcggcgtcc tggactagcg
    13381 cagcgcggcc gtcgactccg caacccgaga cgggctcagc gcgaatcggc ggggtcgggc
    13441 gtgggcacgc cgctcgccat tcggcgttgt ctgacgatca gcggcgccac gacgtgctcg
    13501 atgaagtccg ccgaagtaac gtcagtgtga ttaccgcggt agccgtactc gcggtacttc
    13561 ttgcgatagg gcatgttttg cacgcgcgcg tcatacaaca tgcgcgcgtg ctcgctggaa
    13621 acgtccggca actcgaaccc taacaggccc gcgaccgccg ggctgccgag cagttcgtgc
    13681 tcgcggtcga ccgagccggt gatcggcagc gcgtagcgca gcaccgggtc atccgggcta
    13741 tagaaattat agacccggcg ctttacgcgc cgcagcgcag acgtcagatc gtaagagcgc
    13801 gacaggctgc tggccaagaa cacgacattc tccacgtcca cgccgtcagg caagctctcc
    13861 agggcccagg tggcgatgcc ggttccggct gacaaggcca cgatgttgac cggacgcccc
    13921 gggtaacgcc gcgcgtagct gacgatctcg cgcgccagct cgcgggcctt ctcccggtta
    13981 cgttcatgat cgatctggtc gcgcacggtg ccgcccagcg tgctttgcca cttgaacact
    14041 tcgacgctgc cgcgccagcc ggcgcgccgc aaccccttgg gcacatcgat catgccgacg
    14101 ttgccgaagg tccccgcccc gccaacgtaa taaacgcgac cgtgttgact gccgcgcagg
    14161 tccgtgccca aatcaacgcg cgaaatcgga ttgcagccgg ccgaaagacc cacagcggct
    14221 gcaaacagcg ccaccgccgc gaaacgcaca agctcggttg aactcctcac tctgccattc
    14281 ctttccaagg tcggctcaag ggacttaaat caacggctta ccgatgttct acatcggcca
    14341 cgggaggccg gcgctaccgc cggccgggaa agaccgacgc caccgccgac ggtggtggcc
    14401 ggcgccaggc atagcgccgg cagcgtcgag cgggcaattc gaacgagttg agcattgtac
    14461 atgaaagctg cgccagggtg tatctcacat acgctcggcg ccactggccg gcgtgtcaga
    14521 gtacgtcccg attagctgcg tgccgcccgc ggcggaaaac ggcggcatcc cctatggctc
    14581 aacctgtttc agaacgcctt cttcaacata tatgggcacc ttgttggcga tgcccaaagc
    14641 gattgcgtcc gaaggccggc tgtcgacttc gatcagctct ccgtgcgagc ggaggatgag
    14701 cttggcataa aaagtgtgct cttggagatc gttgatcacg attctttcga gttcggctga
    14761 gagcgattcg atggtgtttg ccagcagatc gtgcgtcata gggcgctgca acggcacccc
    14821 cttaacgcgc cggtcgatgg ctagggcctc tgtaagtccg ataacgatcg gcaagacccg
    14881 cggcccgttc cgctcgcgaa gcacgatcaa ttgctggtcg cgcgtctcgc atatcacgat
    14941 ttgagccaaa tccatccgca cttccatgcg tcggtctccg ctaagcgcca gtatcccagc
    15001 gtgccggggg ttgtcaagca actgcgccgc gccggctggc ttgcccaccg ccgtggtcgc
    15061 ttctagattg cacgcattcc gatcatagga gcacagccgg gtgctgcgaa ttctgataac
    15121 gatcatcatc gcgccggttc tggccgtgct cacgtggctg gccctgggcg aacatgccgc
    15181 gcgacctgat ttcgtggtcg cctctcagga gccacgcacc ctcgaccccc accgcgtgag
    15241 ctggatcgct gagattcaac tcgctaatac gctgttcgaa ggtttgacgc ggctgaattc
    15301 cgacacgttc cagcctgagc cggccgtggc ccaatcctgg agcgtagacg agcgacagac
    15361 aagctggacc ttccacctcc gtccggacgc gcgctggtcc aacggcgagc ccgttacgac
    15421 cggcgatttc cgtttcgcct ggctgcgcgt cctcgatccg gccatcggcg cccagtacgc
    15481 cggcctgatg ttcattatcg acggcgccga agcttactac cgctcgcgtc tgccaggctc
    15541 tgcccagggc gagctgccac ccgaaacggt cgggatcgaa acgccggacg agcacacgct
    15601 gcgcgtacgg ctgagtgccc cctgctcgta tttcctcgac ctcacctcct tcccgacatt
    15661 tgccccggta cacccagcga ccgtcgaacg ctggagctac cgcgacggcc gggctatgcc
    15721 gcagacccaa cacctgtggg tccgaccggg cagaatcgtg tgcaacggcg ccttcgtgct
    15781 gatgcattgg ggtttcaagc agagcatccg gctggagcgc aacccgcact actgggaccg
    15841 gtccaccatc gacgttgatt cgattcaggt acacattacg gccgacccat acgccgcgct
    15901 actcgcgtac gaaaccggca gcgtcgatct cgtgcacggc ctggagtcct cggtcgcgcg
    15961 tgttctgatg ctggaacaac aggccggccg gcggccggat tttcacatcg gcgatcgttt
    16021 cgccacgtcg ttttaccggg tgaattgcct caggccgccg ctggacaacg ccgattttcg
    16081 ccgcgcgtta tcgttggcga ttgatcgcac cgccatctgc gaacgcgtgc tgtgtttggg
    16141 cgagaaaccg gccgaaacgt acattcctcc gggcctggcg ccgctgatgc cgcggcggac
    16201 gccggacggc cgcacgatct actaccagtc gccgcggccg ctgggtgcgg gtttgactaa
    16261 tgcggcgcgg gtcgaactgg cgcgcgagca tctgcaccgt tacatgattt cggcgggcgt
    16321 tgaggatgcc gcgcacattc gaccgataga actcgccttt ggatccgacc ctgagcagcg
    16381 tcggattgct caggcgattc agcagatgtg ggaaaccgcg cttggcctga aaatcgagtt
    16441 gcgcacactg gaggccaagg tcctcagcag ccggctgcgc gatctggact atgacttggt
    16501 tcgcagttcc tggttcgggg actacatgga ccccagcacg ttcctgacca tgttcactac
    16561 agatgatggg cagaacttca cgggctggtc aaatcctact tacgaccgtt tgatcgcctc
    16621 tgccgcagtg gaggcggaca atgaacgccg ctacgagcta ctctccgcgg ccgagcagct
    16681 tctgtgcaac gactgcccca tcctgacggt cttccatcgc cgcggaaatt tcgtgctaaa
    16741 cgagcgattt acgggcttaa acgatcaccc acgggatttg atgccactgc agcgcgtccg
    16801 gcggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaggata tgcctaacgc
    16921 cgcgccgctc cagtagttag cgcgccagct tcctgcgcaa atcccaactt ggccgcgttc
    16981 tcccagtaca gcttccgcag tacctgcgtg ggcagatcaa tgcctctcag ccgcggagcc
    17041 gccggcgcgt cggggtcttc gatcgggctc tcgccgtcgt aggcggtctc ccacattgtc
    17101 cgttgcgccc agtaccggct ggcataatgc tgaaagtcat actggtcgcc tacgacgagg
    17161 tcgctgccga acagcagccg gtccggatgg gcaaggaaca cgtcgcgaac ggcggcgggt
    17221 cgagccgaca cgccgcgaac gatccatttt gtcgcactgg tgtcgaggta aagattggaa
    17281 tggcgcgcta gcaagtcgtt gagaaactcc gggttttcga cacgcccacc catatgagcc
    17341 gcaatcacga aacgcggcgc cacccgctcc aggaaccact ccagttgcgg gtactggtcc
    17401 gccttcgtcc cgaagcgccg cggatcaccg taccggccgc ccggaagaaa ccattcgtcg
    17461 ggatcgccca cgtgaaccat gaagtcgtat cccaggtcca gcgcacgatg aatgaccggc
    17521 cggaagtact cgtggtccag cgttaggccg aactgcgcgc gcaagggtgg cgccacccag
    17581 aacttcatga cacgggcccc caactcccga aaagcagcca gatcggccag ccagcgctcg
    17641 cgaaaactgt gcgactcttc gaaaccgcgc cattgcggta cggctatgaa accgactcga
    17701 tcggcatggt ctgcctgcag cctccgcgcg tcctccatag gggacatgga aattacgcgc
    17761 tccaccccgt acagagccgc cgcttcgaaa aatgcggcgg cgctgcggct caggtgcacg
    17821 tgcgaatgta cgtccagtat tcgcatctcc ggcgcacgag gggccggcgg ttggcggtaa
    17881 tccaagccga gccgattcgc ggggtgtttt ttgtccggcc catcgtggcg gtgagtacgc
    17941 tgcaccacgg cactcccttg cataacctgt cagggccaca gggccttgtc caggttacgc
    18001 gattttattg ttcttcgcag aggatttccg cgtgcgcaat gattcgtcaa gtcgctcaat
    18061 ccgagtcagc gactgcgctg tagggcgtca tcgtaaacag caccggccgg cggccggcaa
    18121 ccagttcacg caccgtagtc gggtcggtca gcgcgtcgtg gttcgcttcg aagaatgtgt
    18181 tgaccgcctt ccagtccggc atcagtaggg cgtgcgtgcc gatctcctcg atttcgtgcg
    18241 gcgcaatcac cagagccaac atctcgtttg ccccgtaagc gcgtgcgtca ctggggcgat
    18301 cgccgacgcc gatcagcaga ttcggccatt tcgtgcgtag atgactcagt acctgcagct
    18361 tgaacttgcc gttatcggcc gccttgcgaa tggcgggcgc gaccaccaac gggcccggcg
    18421 gatagtcatt ctccgccagc caggcccgcg ttttggcctg catcacgcgc gggcggcccg
    18481 tcaggtagac gatgttgaaa tgctccgcca gcttccacaa gacctcccgc gagcccggaa
    18541 tgggctgaga tttcccgtcg agcggactga acagcagagc gcggtagtcg gtgcgagcga
    18601 tcgtgtgatc cacgtccact atcaaagcca cccgctcgtc attcatctcg aacaggcgcc
    18661 ccctgccgtt cagcgacgct ccgaagaccg cggccgacgc cgagaatccc gnnnnnnnnn
    18721 nccgagtcgc ccagttctcc cacgtcgcaa cggatggtcg cgctcccgtc ctcatcggtc
    18781 cgagcttcgc ccaattggtt gtcaccggaa aagaaacgga ccggaacgtt tgaaaccgac
    18841 gttcgcagca atccgaacag ttgatgctca acgtgcgccg tcagttcgac ctggcgccag
    18901 tcatcaactg caaccccgtc ttccagcgtc agcacgggcc gcccacaccc ccccgtgccg
    18961 accatcgccg cacccacagc caacagcagc cgcaccgccc gacggccgcg aaatccacct
    19021 cgtccgcgca cgtctcactc ctggctccac cacgcgggaa tgttaacgcg ctccgccggc
    19081 gactgtgaac ccagcttgtc cggccacttg aaaccgcgat aatgcccaaa tcatgctacg
    19141 cggcttccgg cacacgcttt ttttgctcgt ccagtccggg atcgtgatca gcgccgcgct
    19201 agccttcctt atttaccctg aggcggcccg ggccatcgcc atctgcatgg ccggcggcat
    19261 ggtcgccgtc tggttgagcg aacgcctcgc ccgacactgg ctgcgcgcga ccctcggccg
    19321 cctgcgacgc ttggccgagg acatcggcca gggccgcccc accggtgcgc tggccgtcag
    19381 gaaaggcgac gacctgtaca agctcgtctc ggccgtcaac ctgctcgccg cgcgcgttga
    19441 agaaataacc cgcgaggaga agcgcctcca ggaacaactg cgcaagacgg aacagctcgc
    19501 cttgttgggt gagctggccg ccagcgtcgc ccatgagata aacaacccgc tggacggaat
    19561 tcaaagctgc acgcgcatcc ttcgccggtc gctgcacgac ccggcgcgca ccgagcagat
    19621 gctcgccctg atcgacagcg ggttggcccg catcggcctg atcgtccggc gccttcttac
    19681 tctggcgcgg gagcatgtga tccgcacggt cgaagtgcgc gcccgcgacg tgctcgaatc
    19741 cgccatcgcg gccgtcgcca acaagctcga atccggcggc attcaggtcg ctcgccacta
    19801 cgatgccggc gacgatcgca tactggccga cgcgcagctc ctcgagcagg ttttcgtaaa
    19861 tctgttcctc aacgcggccg acgcgatgag caaaggcggc acgctgacat tgacgacccg
    19921 tctggagcat ccggccgaaa tagtgcccga agcggcgccc cagattctgt cagtggaggt
    19981 acgcgacacg ggtcatggca ttccggccaa tctgctgccg cgcattttcg agcccttttt
    20041 tactaccaag accgccggca agggtaccgg cctggggctg cccattgcgg cccgcatcgt
    20101 tgatgcccac cgcggtacaa tttccgtcaa gccgatcaaa ggcggcggaa ccgtttttac
    20161 ggtaaggtta ccggcgttcc gacccgacca taagcgggct gacgcgactc cgtcatcgcc
    20221 cgccttgctg tcagccgagc cagtatccag cacggttgag tagtttgccg gccgagacga
    20281 caggagcggt gaagcctgcg ggggcaagac gaaacgcccg aaatatcagt cttcagttat
    20341 cggaagagcg gagccggtca ggcggtgcgt gagcgtggac caacctttgt caatagaagg
    20401 tgtcaaaccc ggtagacaag gtgagcgcgt ggagtgggaa tgctcgaccg aggggcgacc
    20461 cagcgatgcg gagctggttc gccaaacgtg cgccggccag cgcagcgcgt tcgatgagtt
    20521 gatcgagcgc taccagcgcc gcgcgacctc ggtcgcctat cgcctgctgg gcgacctcca
    20581 cgacgcgctc gaagtttgcc aggaagcatt cgtccgggcc taccagaacc tggacagctt
    20641 ggaagaccag cagcggtttg gatcgtggct gttacgcatc gtgaccaact tgtctttgaa
    20701 cttccgccgt tcgcgggcgg ccattgcccg caaggcggcg ttcgacgact gtctgaacga
    20761 tggcgagacc gcgcgcgacg agcagattgc cgctccggac tactcggacg agcggcccga
    20821 ggcacgttcg gccgctgcgg agttgtcgct gctggtgcag aaagcaattg ccgatctgcc
    20881 ggaccagcag aagaccgccc tggttctgtt cagtatcgag cagttgccgc agaaggaagt
    20941 ggcctcaatc atgggctgca gcgtcgaagc cgtgaaatgg catgtgtttc aggcccgcaa
    21001 aaagatgaga gaggctctgg ccccgtatct ctaagtcgga ggacgggctg atggaccgcc
    21061 tggcttttga acaactcatt agcacctggc tggacgaccg ggaaaactct gatctgtgcc
    21121 ggcaaatcga cgctgcctgc gcggacgacc ccgcttgccg ccgcctcgtg gcggaatacg
    21181 aaaagctcga ttctctcgtt cggcatgcat ccgatcagcc gcttgccgtc gattggcccg
    21241 ccttcaaagc agtggtttca tctgaaattg ccgcagctac ccaacctgat actgccgcaa
    21301 aactcgaccg ccttctgcaa acggcgctgc ccgccgtcga accacaggtc aactggcaac
    21361 gcttcggcag acgtgtctca caagcaatcg atcagtctgt ggctacccgc cgcgtgctgc
    21421 gcctctcgcg ctggcgcgcc gccgcgctcg gaggattggc ggctgcggcc gcgcttttgc
    21481 tctggttcgg actcccgccg gagcaggcgc ccgtcgcgca gccgagcgcg cctccggccg
    21541 gcgtcgttga gcagccgctg gtcgccactg aacagacttt gcttccggct gcaccggagc
    21601 cgtccgcccg cccgccgaag gtgacgatcg cattggccga gccgcacgaa atggtcgcac
    21661 cgcctactaa agctgcaggc gcgttggccg gccaacgccg ggcggccatg gtccgagtgc
    21721 acgtccacga agaaccgggg cagccgccag cgctggcccg taccgcttct cgccagggcc
    21781 agcgaacatc agaacccgaa gtctttttca tgcttgaacc gcccgatcgc acagtattgg
    21841 cgtctgcggc cggttttggt tactagtgag gtgcctgtaa atgaaccgca cccgtcgttt
    21901 ttgctggctg tcatggatgg cgccggcgct gtgtctggcg ctaaatgggc cgctggctca
    21961 agccgtcgat ctgcgtgatc tggtcgaaga ggtgttggat caacccgccg aaggcgtgga
    22021 gatcggccca ctgccaataa accaggcgct cgagcaactg ggtgagcgga ccggtctacg
    22081 ctttgaactc gacgcgcaag cgatcgcctg catgccctat ggcgagaaca cgcgcatctc
    22141 cttggcgttc tccggcctca ccgtgcgcca gggtttgcgg caagtgttcg acgggctagg
    22201 tttggcgttt gaagtggtgg acgatgaggt tcgggtcgta cgcgggccgg tcttggagcg
    22261 tctgggccgg cgcctcgcca ttgaagaagt acgtacgtta cagcaactcg caacgcataa
    22321 atggtcagac ctggacccga agtggcgcca actccagttc cgcggcgtga aggacgcaaa
    22381 tgcggccgcg acgctcgaaa atgcgatagc tggcgtcatt gcgggcaatg cggtgcgtca
    22441 gctcgaggcc gccacgcaaa gcctgaattg gctttggacg ccgctggata cttcaatcct
    22501 ggtgtatagc caggcggaag atgtttggaa ccgcctccag cgccgaattt cgctgaacta
    22561 ccacggactg cgtttggatg acctgctgat cgatctgggc gagcgcgtcg gcgtcacgat
    22621 catgtttgaa tcgggcgttt tagagcgcat ccaggccgat gaccgcgccg tcgatctcgt
    22681 acaccagaac actactgttc tgcaaacgct ggagcgcatc tgcgnnnnnn nnnnnnnnnn
    22741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnngtcgat ctcgtacacc agaacactac tgttctgcaa
    22861 acgctggagc gcatctgcgg cgctacgcac acctgcttcg aagtccagga ttacggcgta
    22921 ttgatcgcca tgccggccga cagcgagcgt cccggcctgc gaacgccgcc cgcgccgccc
    22981 gccgtcgagt gccgggtcgt agcgatcctg cgcgtgccgg tcggggacga cggcacgacg
    23041 gtggatttcc ccttctacga acacaatgtg ccgccggaat ttcggcgctt gctggagcag
    23101 aagctgccgg tcgtcattga cgaactgcgc aaaggctctt gagccgcatc gcctccgacc
    23161 ggcgccggcc gcttccatca ggcatatagg ccgcgcaggc gcatgctgct ggcgacgcgc
    23221 gcgatgccga cgcacagggc ggcagtacgg gtcgaggtct ggtgctcacg ggcgaattcg
    23281 cgtgcccggt gatagccggc gcggatcaag tccgagaggc gcgtgtcgat ttccgcggcg
    23341 ctccagaaga agttgatgcc accctgtacc cactcgaagt acgacacgat cacaccgccc
    23401 gcgttgcaga ttgtgtcggg gatcacgaag acgccgttgt tctcgagtat cggatccgct
    23461 tcgggcagtg tgggcgtgtt ggcgccctct gccaggatgc ggcacttgat gcgcggggca
    23521 ttggtgcgag ttatgacgcc gccggtcgcg gcgggaacga ggatgtcgca aggaatggtg
    23581 ggcacgtcga cggcggggac gcgctcggcg tccgggaagt cctgcaagtc accgtgccgc
    23641 cgtacgtggg ccagaagtcg ctctacgtcc aacccccgct cgcagacgta gccgccggtg
    23701 atgtccgcga cggccgttag gcggacgttg cgccgcgcca gctcagtcgc ggcgtggacc
    23761 ccgacgttgc caaagccgtg cagaaccgcg gtggcgccgt ccagcttcag accgatctcg
    23821 cgcgcggctt cttcgatgca gtagaccacg ccgcggccgg tggcttcctt gcggagcacc
    23881 gaaccgccga cgtcgacggg ctttccagtg acgacgtgcg gagtgaactt gccgacgtgc
    23941 tgggaataag tgtccatgat ccaggccatg gtgagctcgt ccgtgcccat atcgggagcc
    24001 gggatgtcga attccgggcc tatgaacggg gcgatttccg cggtgtaccg gcgtgttagc
    24061 tgctggcgct cgttttcgga gagttggcga gggtcaaccc gcacggcgcc cttggcgccg
    24121 cccagaggca ggttcatgag gccgttcttc caggtcattt gcgtggccag agcggtgacc
    24181 tcgcccacgt cgacgtcggg gtgatagcga attccgccct tgtacggtcc caaagcatga
    24241 ttgtgctgga cgcggaagcc cgtgaagacg cggatgcggc catcgtccat ctggaccggc
    24301 accgaaacca tgatgacctt gtcgggtcgc gcgaggcgaa cggcgaggtt gtgctccagc
    24361 tcaagcacct tggcggcctg catgaagttg tgcataacgg cttgaagcgc gggagattcg
    24421 ccgaagatca tgtcgatgga cgcgacgggg gtggcctctt tcatagtctg agctcgcgag
    24481 ataaccgacg cctgaggcgc gttcacgcag atagcgcctg aacggagtgt atgcggcctt
    24541 acgcgcggcc ggcgggcggt cagagcagaa gagccggggc gtgtcgccag gagcagagca
    24601 gagccggggc gtcgccccgg aatgccgcct agcacgccgc tggggtcttg gctgcctttc
    24661 cgttataatg cccgcccagt ccccgccgtc ggggccagag ccgagcacag cgccgccggg
    24721 cggcggcgcg aacggaggaa gttggagttg atgacgacgc gcgcaaccgc cttgccgctg
    24781 ctggcggctc ttatcgcgtt gatatcgagc gcaggctgct cgcacattat caagcccgct
    24841 tactacgagc tgcgcggcgc caagggcacc atttattggg tcgaggaacc gcccgtcagt
    24901 tcgctcgatg agttccgctc cgtttatttt gagccgctct cgactacgct gggcgccagg
    24961 ctatgtccgc cggaactcct ggaagaatac aaccggaccg cgcgcgtcta tgaggcgaag
    25021 ctcgagaact acccgggcgg cagccctacg ctgagtgtgg gcggcgacgt ggtttacttc
    25081 caaaataaag gactcctcag cgcggcctta ttgctgatgc gcttcaagct gaccgcggac
    25141 ggccgccaag tcggcgacgg ggtgctacgc gtcgaaagca agtccttccg cgaaggcgac
    25201 atcggggacc tggccgaaac cgccgtcgag accctgtcca agtacctcga tgaagagaaa
    25261 atgccgcgcg acgacgatcc tgagcgcagg cgggcgcgcc gtccgttcgg ctggtttaac
    25321 aaattgacgc gagcgcctgg ttgagatcgc caagcgggca cggagcccgg actccagcta
    25381 tccattagcc atttggtatc catttagtgg gctacccagc ccgaccttgc ggctggtcaa
    25441 aaaatgtcgc aagtcgatgt accgcaacaa tttaggaggc ccaagcgtgg gcacgggcgc
    25501 gattcgagag taatcagcac acgaataccg gacgttgccc gaactgcccg gcccaggccg
    25561 atgcccgcat cgaaaaaaca ttgatccgag catgccattc gctatgattg gttcgggttg
    25621 aatcgctgat gcgaacgggc cagaagtgat gccacgcgcc tttcagatca gccggcacgc
    25681 cgccgcgaca ttagcggtta tggttctggc cctgccctca ctgttccccc aaccctgcca
    25741 ttgcccttgc tgtgagacca gggctcctga cgccaaggtc actgcctccg cgtctgataa
    25801 gacaagttgc ggccaaacca agtgcccgca ttgcgatcgc gaggcacccg gcaaggccgg
    25861 ccaatcgcaa agccagggct gctgcaagaa ctgcaccgct gggtgcagct tggacaagcc
    25921 cacctaccag ctttcgactg attcgatgtc ggaactctgg tcgttagcgc ccgtcggcct
    25981 ggtcgcccct caaacgctgg acgagccagc cagtccctat cttccaggcc tgctccgccc
    26041 accgcgcatc tgatacttcg ctcacactct tccaacgtac gagccgtgct gaactgcgac
    26101 gcgctgcctg agtcgaatcg acttcgcgtg cgccacgttc agcagaaaaa agagacatca
    26161 ggagccaagt atcatgacac aagcgctttc agatctatcc gtcctggcgg gccgccctgc
    26221 ggaccgtgag cccgccggcg aggaccccga ggcccctcgg agcggggccg gcgtacccgt
    26281 acctaaggcg cgttggaaaa cgcgcgttct gctaccgggg gcaattctct gcataaccgc
    26341 cgccacgttg ctgatgaccg catggcgcgc gctctggccc gctacgcccg tgcgcgtggc
    26401 gccggtcgta cttaagaccg gcgtgcagcc cgccggcaaa gtcatagtcc aggcccccgg
    26461 gtgggttgag gccgacccgt tccccacggc agttaccgcg ctggccgacg gcgtcgtcgc
    26521 cgaggtgctc gtgctcgaag gccagcgggt cgagcccaat caggtcgtag cgcgcttgat
    26581 cgccgacgaa gccaggctcg ccctggccaa agccgaaagc gcgctgcacg ccgccgcggc
    26641 cgacgtcgcc gctgcgcagg ccgagctcaa agccgcgcag cgcgaatggg acaacccaat
    26701 cgagctgacg cgcaaactgg ccacttcaca ggcggaactg gcccagcgcc aggcggagct
    26761 ggcgcgctgg ccggccgaac tcgaggcggc aaccgcgctg gccgagtaca agcaagctga
    26821 gtatctgcgc gtcaaaccgc tgcacggctc gggcagcgtc agcaacatcg aggtgatccg
    26881 cgccgaaaaa gagtatgaga accaaaaggc gctggcgcag gccgtgcgcg acatgaagcc
    26941 gattctcgaa gcgcagatcg cccacctgga ggccgaggtc acggcggccc gcgaaaacct
    27001 gcggctgcgc atcggcgaga cgcgccggct ggaagccgcg caggccaacc tcgagcgcat
    27061 gcaagctgcc cggctggtgg cggaggcggc ccgcgacgag gccgccctgc ggctggaacg
    27121 catggaagtg cgcgctccgg tgggcggaat cgtgatgaac cgcctggtcg agccgggctc
    27181 caaactgatg ctcggcggca cggagatgga ctcagcgctc gtcatgcgcc tgtacgaccc
    27241 ccagaagctg caggtgcgag tcgacgtgcc gttgactgac gccgggcgcg ttggtgtcgg
    27301 ccaacgggcc gaggtcgtcg tggacgtcct gcccgatcgc gttttccagg gtcgcatcac
    27361 acgcatcgtc aacgaggccg acattcaaaa gaacacgttg caggtgaagg tagcgatcgc
    27421 cgaccccgcg cctgagataa aacccgaaat gctggcgcgg gcgcggttct tttccgatcc
    27481 ggaaactgca tcggatacgc cgggccagcg gctgtttgtg ccgcaatccg cgatcgtccg
    27541 gcacgccggc gggcacacga tggtttgggt cgccgatcag gcccgcaata tggcgctgcc
    27601 gcgcaccgtg accgtcgcgt ccaccacggc cgacggctgg gttgaaatcg agagcggcgt
    27661 gcaacctggc gatcgcgtga tcgtcgaccc gccgcaagac ctcgacgacg gcgctcgcat
    27721 tcgcatcgcg ggggaaatcg gcggggattc attttcaccc acgaaaggaa tcggacatgg
    27781 cgctcattga atgccgcagc ctgacacgct cttaccgcaa gggcgatatc gtgataacgc
    27841 cgctggatgg cctgaacctg gacgtcgaag aaggcgactt cctggccctg atgggaccat
    27901 ccggcagcgg caagacaacg cttttaaacc tcatcgccgg aatcgaccgc cccaccagcg
    27961 gcgaactata cgtcgacggc acggacatct cgcaactgtc gcgcgctcag ctcgccagtt
    28021 ggcgcagcaa atacatcggc tacatcttcc aactctacaa cctcgtgccg acattgaccg
    28081 cgtacgagaa catcgaactg ccgctgctgc tgcataagct gtcacgccgg caacggcacg
    28141 ccaaggtcgc tctggcactc gagttggtca acttggccga tcggcacgac cacttcccgc
    28201 gtcagctctc cggcggccag gaacaacgcg tcgccattgc ccgcgccatc gtgacgaacc
    28261 ccaagatcat cgtggccgat gagcctaccg gtgacctcga ttcacacgcc gccgccgcca
    28321 ttatggacct cctggtccgg ctcaatcgcg agctgggcaa gacgttgctg atggtgacgc
    28381 acgacgcccg ctgcgcggcc tacgcccgtc acacgctgca cctggagaaa ggcgcgctag
    28441 tcgaggcgcc cggcgcgacc gtcgccgcct gaggagaaca acttatgttg gcccccaagt
    28501 tcatgccctt tgtcctgaag caggtcgtgc gccaccgcgt gcgcagcctg ctgaccatgt
    28561 gcggcgtcgc ggtcgcgatg ttcctgttta ccgcagtgca gtcgatgcag gccggcgtcg
    28621 aagccgcaac gaccgccaac gccaccgaca acaccttggt cgtttatcgc aaggatcgct
    28681 actgcccctt tgccagccgg ctgccgcaat cgtacttcga gcagatcaag cggctgccgg
    28741 gcgttgaaag cgtcgtcccc atcaaaatcg tcgtgaacaa ctgccgcacc agcctcgacg
    28801 tcgttacgtt ccgcggcgtg cccgaggagc cgttcatcga cagttacgca ccgaggctgc
    28861 ggctgctttc cggctcgctg gacgactgga agcggcgcag cgacgccgtg ctcctgggtg
    28921 agacgctggc ccgccgccgc ggcctgaacg tcggcgaccg cttcgaagca gccggaatca
    28981 ccgtgtatgt ggcgggcgtt gtgcagtccg atgagccgca ggacgagaac gtcggctata
    29041 cgcatctcga tttcctgcag ttcgccacgg gcagccgcgc cggcggcatc gtcacgcaat
    29101 tcaacgtccg ggtgagcgac cccagccagc tcgagccggt ggcggccgcg attgatgagc
    29161 tgttcgccgc cgaccaggaa ccgaccagga cgagtccgga gaaggcgttc gtggcccgcg
    29221 cggctgcgga tgtgatcgaa atcgcgggct ttatgaagtg gctggggctg ggctgtctgc
    29281 tggccgtact ggccctggtc gccaacgcca tcgtgctctc ggttcaagat cgggtccgcg
    29341 agcacgcggt gctccaaacg ctcggctttc gcggcaatct gattgcgcgc ctgatcatcg
    29401 gggagggtct ggtcatcagc gcgctgggcg ggctggtcgg ctgcgcgctg gccgccttaa
    29461 ttctcagtcg cggcggcttc gcgctatcgg tggatgcgct cagcattccc atcaagagcg
    29521 gactgcccgt ctttctctgg agcatgctga tcgcgtgcac gctgggcata ctggccggcc
    29581 tggtgccggc gtggcaggct tcgcggcggg agatcgtaac gtgcttccga gcggtgtagt
    29641 gacgcccgcg ggctgcgccc tgccgagccg agccgcggct cgccgggaag ctcgccttca
    29701 aggagaatcg agtgaaactg ctaccctttg aatacgccgt gcggaacctg ggccgctcgc
    29761 ccctgcggct ggcagccagc ctgatcggcg ccgcgctggt cgtggctctc gtgctcgcgg
    29821 ccggcagctt tgtgcgcggc atggagcgca ccatgtcggt cagcggaagc tccaagaacg
    29881 tgatactgct gggcgccggc agcgaggaaa gcatcgaacg cagccaaatc ggcctgaatg
    29941 tagccggcct ggtcacgggc agcgtgcccg gcatccgcac gaacctgggc gtgccctacg
    30001 tttcgcccga ggtgcacatg gcgttactcg tgcgcaccgg gcgcgaggcg gaacaggagc
    30061 tgaatgccgt cttgcgtgga ataaccaata ccgcatacct cgtgcacccg caggtgcgcg
    30121 tggtcgaagg ccgccctccg ctacccggcg agtacgaatt gatggtcggc agcctcgcgg
    30181 ctacacgcat gggtgttcca tccgaagccc tcgcagtcgg ccgttcgctc tggtttgata
    30241 atcacgcctg gacgataacg gggcggtttg aagcgccgca caccgtgatg gacgccgaaa
    30301 tttgggtccc gctgacggat ctgcaggttg cgacccgccg cgagtccacc atctcctgtg
    30361 tcatcctgac gctcgacgac gccgaatacg aggatgtggc cgccttcgcc gctcagcgcc
    30421 tggacctcga aatccgtgcc gtgcgggaat cggactacta cgccagcctg ctgaagttct
    30481 accgcccggt gcgcggcctg gtctgggcca ccgcgattct catcgctctg ggaggcctgt
    30541 tcggcggact gaacaccatg tacgccgcct tcgccagccg cgtgcgcgaa ctgggcgcgc
    30601 tgcagacgct cgggttctcg cggaccgcga tcggcgtcag cttccttcag gaatctctgt
    30661 tggccagttc ggccggcagc ctggcggcgg ccctgatttg tgtgccgctt ttgaaccgcg
    30721 tcgccgtcaa attttcgatg ggggcgttcg cactggttct tgacgcgccc gtactgatcg
    30781 gcggactggc tgccggcgcg gcgctgggaa ttattggaat tatcccgcca ttgtggcggt
    30841 gtttgcgact gccgattgtg gagtcgctgc gcactgcata gagaaaggaa aacgagatgc
    30901 gaacactgat aacctccacc ctgttgctga acctggcgat tctggcgggc tgcgatcaat
    30961 ctgcacaatc gcccccccct gccaagcccg ccgcatcggc cgcgtccacc cccgccgcac
    31021 ccgattcggc tctgccgccc ggcttgtggc tctcggccga accggcggat gccaaaccgg
    31081 tcggcgaaat caaaaagaac gccgccgccg gccaggacgt agtgctcttc gggcgcattg
    31141 gcggcagccg ggacccattt gtcagcggcc gcgcgatgtt cacgttggtg gatcgcagcg
    31201 tgccatcgtg cactgaccgg catggcgacg gctgcccgac gccctgggac tattgctgcg
    31261 agccgcgcga gaacctgctg gccaacaccg tcacgatcca ggtcctcggg gccga
//
LOCUS       DAOSYS010000010        29481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1301_length_29481_cov_5.395908, whole genome shotgun sequence.
ACCESSION   DAOSYS010000010 DAOSYS010000000
VERSION     DAOSYS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 29481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29481
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1301_length_29481_cov_5.395908"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..580)
                     /locus_tag="PLU99_01305"
     CDS             complement(254..580)
                     /locus_tag="PLU99_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15720.1"
                     /translation="MSKRTIGLAAVGLLGLGLAAAPAEAGWGFGFGFSWGRPAYYAPV
                     VYPAPVVVPAPATVVYEYPAVYGGPVYYAPVVAPARYYHARPYYYGVRGYYKHYSGGH
                     HKHYHR"
     gene            891..1754
                     /locus_tag="PLU99_01310"
     CDS             891..1754
                     /locus_tag="PLU99_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOO15721.1"
                     /translation="MPQEVVCINGQIVPADQAGVSVFDAGFMQGVGLFETMRAYGGRV
                     FRLERHLDRLANSARELGWTLIPDHEQMIDCVNAVLAQFAGQDARVRLTVTTGSLRAA
                     EAGAVRLTMVATASPGGKYADTFYEQGVEAVISRYRQSRFDPLAGYKTTSYFGRLAAL
                     REAHGKDALEAIWLNDQALVAEGAISNVFAVREGRLVTPPLATPILPGITRATVIELA
                     AEAGIAVDENDLTVEQLKSSAEVFLTNSMMEVMPVVRIDRDAVLQGRPGEVTRRLAAA
                     YSELVARECGR"
     gene            1751..2923
                     /locus_tag="PLU99_01315"
     CDS             1751..2923
                     /locus_tag="PLU99_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOO15722.1"
                     /translation="MSRAQTPREVRKAGAAETAAVTVATLASALNEIAPPYLAAEWDN
                     VGLLTGRPEWPVTRVLLAIDLTDAVAREALETGASALILYHPPIFKGIRNITPQAECP
                     TALLPDLLAARISLLAVHTALDAAVGGTNDVLLDFFEPVSRRPLELHIEERRTYKLVV
                     FVLPNEVDALRRALSDAGAGVIGHYSECSYALAGQGTFRGDETTRPTVGEKLRLETVD
                     ETRLEMVVPRARLGEVVRALYATHSYEEPAFDLYPLHETPGRAAVGMGRVGILPRPER
                     GDVLLGKLRRHVDLSAALVVGRLKRSFRSVTAAAGAFGVRAFADTDSLVLTGEFKHHD
                     ALELQKRGVTAVHLGHYASERPALESLRARLSERVAEVTFETARADKSPLSPVRLE"
     gene            3049..3354
                     /locus_tag="PLU99_01320"
     CDS             3049..3354
                     /locus_tag="PLU99_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO15723.1"
                     /translation="MNAEPRIPIDASKIAAFCRKWRVTEFALFGSVVRDDFRADSDVD
                     VLSFERDAPWSLWDLLAMRDELGELFGRRVDLVEREALRNPFRRKEILSTRQVIHAA"
     gene            complement(3562..6405)
                     /gene="polA"
                     /locus_tag="PLU99_01325"
     CDS             complement(3562..6405)
                     /gene="polA"
                     /locus_tag="PLU99_01325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOO15724.1"
                     /translation="MARTLYVIDGHSQIYRAYYAPFGNLSSPSGEPTRATHVFSQMLL
                     NLLRNRRPDYLAMALDVDDRTVFRVQIYPEYKAQRQLSPEDLPVQMDRIISIVQAVGI
                     PILRVPGFEADDIIATLAARLAGPDLTLYAVSRDKDLDQILSPHVALYDPLKDEVITA
                     ERLPELKGWTPEQAIDAQVLIGDDVDNVSGVAGIGPKTAAKLLQKYGSAEEVIAHAHE
                     LTPKQRENVLAFAPRLEISRQLMTLRRDVPLEFDLEQARCERINWSAAEPIFRELGFR
                     RLLDQLPAAARAKNDTANATTPAAPDVAVGTNPQPQSPAHASSLFEQSSATPAPAIPA
                     ISRPEVAELSQPEGGDYRLVNTPENLDELAAELARQPEFALDTETTAINPIDAELVGT
                     SFAWKPGQAYYVPVRCAFGAALPLDLVRQRLGPILADPKIRKVGHNLKYDSLVLRLAG
                     LPLNGPLFDTMIAAFVVDPARASYGLNSLVFGLLGHEMIPITDLIGKGRDQLSMDQVP
                     LSRITEYAAEDADYTWRLRMLLEPKIQATGVADLFYETEMPLVSVLTDMELEGINIDV
                     NFLAAMSRQMAERAEALCDEVCRHAGRRFNLDSPRQLAEILFDELKLRVVRVTKTARS
                     TDADTLAVLQRETGHAMLKPLLEYRELQKLRGTYVDALPLARSRRTGRVHTCFSQTAA
                     ITGRLSSSDPNLQNIPIRTELGREIRRAFIPRSAQGRLIVADYSQIELRVLAHFCQDE
                     GLIEAFTEDRDIHAFVAAQVNGVPLAEVTREMRARAKAVNFGIIYGQGPFGLAQTTGM
                     SRTEAKEFIDAYFRRYPRIQAFIRQCVAEARRNEYVRTIQGRRRPIPNINSRNNTARA
                     QAERLAVNTVIQGSAADLIKTAMIRLHQRIKSERLPLHMLLQVHDELVFETPAQEVER
                     MSAIIRDIMTHALPLTVPLKVDIASGANWLEAK"
     gene            complement(6620..9340)
                     /gene="gyrA"
                     /locus_tag="PLU99_01330"
     CDS             complement(6620..9340)
                     /gene="gyrA"
                     /locus_tag="PLU99_01330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOO15725.1"
                     /translation="MSATPTAGAPITPQSIPTPISQEMQESYLTYAMSVIMARALPDV
                     RDGLKPSQRRILVAMNDLNLHPRAKHRKCAKICGDTSGNYHPHGEGVIYPTLVRMGQE
                     WSMRHLLVDPQGNFGSIDGDPPAAMRYTEARLMTPAELMLEDLDKETVDFEDNYDGTR
                     KEPVVLPGKFPNLLVNGSEGIAVGMATRLLPHNLGEICDAVVACIDNPNITIDELMKI
                     VPGPDFPTGGVICGTAGIKEAYETGRGSIKVRGVLHTEELKNGRIQIVVDEIPYGVLL
                     PTIKERVNEAVESGLIKGIEDLRDESGREQLVRLVIPLKKDANVQVTINQLWQYTPLE
                     SHLHAINVVLVNRVPRTLGLKQLIEHFVRHRVEVIRRRTQFLLRKARQRAHVLEGLIL
                     AVADIDEIIRLIRESPNADVARQRLMEKNLRLSEAATVRALLPAVFVAKATAADQRLT
                     HTQADAILAMQLRRLTGLEIEQLAAEYTKLVEEIADYELILSDERRVLDIICEDMREL
                     KEKLGEQRRTQIGGDVGTLELDELIAQEDVVVTISHEGYIKRVPIDTYRSQGRGGKGI
                     RGTEARDGDFIEHLRVASTHDYLLFFTNRGRVYWLRVYDIPSMQRTSRGRALANVLTL
                     QPNERHVAVLSVSQFEEQYAFFATEKGIVKKTPLRAFSNPRPSGIIAMSLDLDDSLIK
                     VSLSNGAQQIVLGTRNGMACRFNEDEVRPMGRSARGVRGIALTGDDRVIDMVVIEPGM
                     SLLTVCENGYGKRTDIEEYRLTHRGSKGVINIRTTERNGPVIAMRAVTDENDLMLITA
                     KGTLLRMPMSDLREIGRATQGVRLIRVDEDDRVVAIARVVSEKEENAINGLSPAGEGA
                     DKSADILTQTPAANAAAAGNGEVREPDDVEPEAPAESDEAGENGDSEPQA"
     gene            9735..10421
                     /locus_tag="PLU99_01335"
     CDS             9735..10421
                     /locus_tag="PLU99_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HOO15726.1"
                     /translation="MKRSYRWMWSGCALMAAVALAVQGFTQEKKPDQPAQPETKPPQV
                     QPEKKPGAPAPTEMTPEEMMKLWQELNPLTPQHQEMAKAVGKYDVLVRYRMAPDAPMR
                     ESKGKAEFKLILDGRFIEQTYSGDMEGVPFTGLGIEGYDHLKKQFQTVWMDSMSNMMM
                     HTTGTASPDGKVITYTGKMDDPMTGQKDKPIKMVLRKISDDKTVFEMYDTLPDGTEFK
                     SMEMEYTRQK"
     gene            10773..15962
                     /locus_tag="PLU99_01340"
                     /pseudo
     CDS             10773..15962
                     /locus_tag="PLU99_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            16003..16659
                     /locus_tag="PLU99_01345"
     CDS             16003..16659
                     /locus_tag="PLU99_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15727.1"
                     /translation="MIEAICRRAFFQFEFPIHYRARTPVIDGDVSKWGPHFLVPALVE
                     MEDQPPFADVYCAWNADHFMVAFDVPERWGPLRCDQTQWWKLDGVRICIDTRDTRDAK
                     RATRFCHFFYVLPTGGGPRRNQPVIGLHRMSRAKEPPPAVDLAQIQLAARTARRGWSV
                     EVAIPASSLSGWNPSEHPRIGFFYKVKDTQLGSQHLTVDDELGWNADPSTWATGVLVK
                     "
     gene            16838..17752
                     /locus_tag="PLU99_01350"
     CDS             16838..17752
                     /locus_tag="PLU99_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15728.1"
                     /translation="MMKRCFDIGVVVVALLAVPLALAQTGSAGQSGGSQWTTGQYGQM
                     GAEPGHPGQYGHTGGQYGYTGGQYGAQYQPGAMYGGQMGAYQGQSGAYGSQYVPDFGY
                     GQFGSYGSPYIPEWSYGGGQRGYWQGGMYGQTGQYGQFRGTQYSSPYTYTGYPQYQGM
                     QYHSGQSGQGFRGGYYPGYPSGQPSGGYSGQPDWYSWQNRYNQPDWSQQGVWRQRSQQ
                     PYGYSGYGQQDYYRGSYYPSSAWPGYGNPGWHNQPASYTQWDCRSMPCPQGYGTVGHM
                     RCDNLYGTPCYNLINPYNGSVAHEAFER"
     gene            18085..18621
                     /locus_tag="PLU99_01355"
     CDS             18085..18621
                     /locus_tag="PLU99_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15729.1"
                     /translation="MGKDYFAILGLTPGRYSGPEIARRFHSERARLLAQLNEPALHDA
                     VCQRLDELHLAYAALHDPQGQERALRLRGAAADQIAALKMLIAASLEDGLLRYSRRQH
                     ILQEARQLGLSDFQAHLLIAEVQFADDDFAADWVRAGELPAVTRPVERRRGAGRRLVA
                     AGVLALGIFVALVRWFAA"
     gene            complement(18623..19531)
                     /locus_tag="PLU99_01360"
     CDS             complement(18623..19531)
                     /locus_tag="PLU99_01360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO15730.1"
                     /translation="MSDSPLSPEAPQPEVVFARPVEMPPGALPGRLPGGAVRARAVSA
                     AQTPASATCLTLPGLPLRAAVVDFLLLLLVAAVALGGPSIVVGTWMAATGRELPDQPT
                     FRMMMSQQLFQVILSIGLLSFLLWRRNLPSCAFGVQAHMPGQQLLWGAAGLGVAYGWM
                     IFSVIVISAVLQLFPGLQSDLTKRQDLIELLPDTLGKVIILMIPVAVSEEILFRGLML
                     PYLRRITGYWWSAVLISTVIFAVLHFQQGILGILQISGVAVVFALIFIYSRSLLAAIV
                     AHFAFDVGQMLLVMLSKDMLKSYGLE"
     gene            19818..21521
                     /locus_tag="PLU99_01365"
     CDS             19818..21521
                     /locus_tag="PLU99_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15731.1"
                     /translation="MSGNAGNLSGPRGGLRPGAGLTRGWHWERVGELLVSNPVRCKRD
                     QCYLARGTAADGMRPVLAEFAIRFLQDGRELAQRRLALHARNGHDGELLGWIQTPENA
                     THLQILVPRAASANVLGEVTFHAVAERDPKCHPLANVPRWNTYRPPFPIQRVLLPASL
                     AELASAVPELAVTHVRPRSRGALAHQANQAACVLDPAWVDGLGLTWEDLLRLADRCWL
                     LVDLETAGKLLSRAGVRGLELFTDEAQHGLMSARVEYADVQTRGFALQDVFPYGALTP
                     AGGFCVRALRANRAWRRLADELALATLLASETPSPRQTGHVLSAAAALGRGELVITDL
                     PWLVAGSMGPPIAPRLAQHALRMHLGAPVEDDLQYWNRWDDSQIVVRDICDLARRYPP
                     LQTVRWTPSPEGNACLGVYLPAQNRAARATTVLIHTGRIDNLDLHGGLPPEPMMIIMK
                     QLAREAREATAWAQRYLANVNVVWQFDTAAGLRYAVNYASAESFDFGTVYSSHLHPLN
                     GRKDCTPSSVRNGAATRDNPARGESALLTQGEGIYGDGSLDYLRQLMSHVRGLIEAQA
                     V"
     gene            complement(21819..22877)
                     /locus_tag="PLU99_01370"
     CDS             complement(21819..22877)
                     /locus_tag="PLU99_01370"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOO15732.1"
                     /translation="MQRRYSATGYDITPLSREEVRQLAAKLDPETYRITQKSGTEPAF
                     CGTLLDNKQDGTYCCVVCGLPLFFSAHKFTSGTGWPSFYREFDPHHVSRKTDRSAGMI
                     RTEINCARCGAHLGHVFDDGPQPTGERHCLNSVSLKFYTKSEALPPESRPAARPGSEP
                     ATPPATQSAGAPSTDASRETAYFAGGCFWGIEHYFQQGPGVIDAVAGYMQGHVDRPTY
                     KQVCNGRTGHAESVKVVFDPQRITYRRLLEAFFKMHDPTELDRQGPDVGSQYRSGIWY
                     TNEQQRREAEAYIAELTAARAFGGRKIVTQVEAAKTFWPAEDYHQDYVEKTGRVCHVA
                     DPWQDLRDRAQPAATSRP"
     gene            complement(23008..23976)
                     /locus_tag="PLU99_01375"
     CDS             complement(23008..23976)
                     /locus_tag="PLU99_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15733.1"
                     /translation="MTESPELRPAGSSPGAVPRPWLHTVVTGGLLILWCGVIIFAIIT
                     WINPPWLAEMSRPGRKTEADWYSAAGVTRLRNNDFAGAISAFKQALAVNEDTQTRVHL
                     AIAVARSGQVDAGVRLLEQALQENPGKFLRGVILFNIAEMIWRNPNNYEVAKRLYQEA
                     LECGAEPDKVYRRLGVILLSQNKPQEALVAFEKTRQAQLDLTQPYRNMLQRAMEGLSV
                     VSTEELEAQAAAGITEEELRARYDVQTLLAVQQEDNPEMSETHKMLGNVHAMLGDFAR
                     AQQEFQEALRLWPGNQEAAINLERVTAVMQQLADVKAADGQPDPHP"
     gene            complement(23969..25210)
                     /locus_tag="PLU99_01380"
     CDS             complement(23969..25210)
                     /locus_tag="PLU99_01380"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOO15734.1"
                     /translation="MSTAVTTRYAPSRLEWIHSTRLHLVATALLVVCMPFLMLRAYLQ
                     DAIGRASAATFPFMGVDVPWVLVVASVVGVGLLALLWPYLNRGRLVAAGIAVLMIAYG
                     QYINDYYFGHEFYELQLNWHYFAYMFFAVILYRDLTPRGYKPATIIGLTVGVSLALST
                     FDELFQTFVNNRFFDTGDISKDVWGGVMGLLLVYNGSSELRSWRPLRHRRLSEYFRSP
                     GSMMLLLGVTAWGLLTYCSLLNIADEIPITIYLTIGTFVVTFLILHLSQFRPWRWAMI
                     TIAVLAIGAQAWALVHYRDSGMVYWRPGLAVYRGLVWPYFDFAILPNGTLHPATKLHE
                     FNPRDRGFFLKQCADIILIGAGPHGEGGHGFMSRKTHFMYNPNTKRGSQVIIQPTPQA
                     CETFNRLKKEGKNVLFVVNND"
     gene            26100..27647
                     /locus_tag="PLU99_01385"
     CDS             26100..27647
                     /locus_tag="PLU99_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO15735.1"
                     /translation="MTDILKKLNISENYPGGFCGEWLGSGPTLDVITPVDGSHIGSVR
                     QVTEAEYDRIVARAHEAFLSWRMVPAPKRGEIVRQLGVRLREYKEELGALVTLEMGKI
                     AAEGAGEVQEMIDICDFACGLSRQLYGLTMHSERPRHRMYEQWHPLGVVGVISAFNFP
                     VAVWSWNAAIAAVCGDSTLWKPASKTPLTAIACTRIAQQVCEENNVDPAIFSLVVGPG
                     RTVGEKLLNDRRIPLVSATGSCQMGYRVGEVVGKRLGRTILELGGNNAIIVTPSANMD
                     MAVRAILFGAVGTAGQRCTSTRRIIVHESVRKDLVNRLTKAYASVPVGDPRKSSTLMG
                     PLVTHQAVEDMQNAIRRVKEEGGEILFGGEPLSGPAYPGGHYVKPCIAAAKNEFRIVQ
                     EETFAPILYIISYGSAGGSPEKELEQALALHNGVPQGLSSAIFTTNLLEAETFLSHLG
                     SDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWSTELPLA
                     QGITFGS"
     gene            27920..29212
                     /locus_tag="PLU99_01390"
     CDS             27920..29212
                     /locus_tag="PLU99_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOO15736.1"
                     /translation="MYACVAAVGLAAALCAKGRAAEVIVKNDSFTEGSPAYLVGDFVP
                     GEEVGVRLTSPCDGTIVGVQIAWLEGTPGHPPALHRAIRIYDGSTFPTPGAQLAYLEA
                     PLLSPGFVNEFRYLDEAGTVPLSVPVTVGQQFYVALEFDEPTDVGSGGASVVRDTDGC
                     QTGRNIIYVIPGGWFNFCLFIQGDVVIRAVVDCGEQSGACCLPSGACQFITPTQCAGS
                     AGTYHGDLVSCAAVECPQPSGACCFEATGGCLDLTQQLCGVTAGIWAGMGTQCATYTC
                     FPSGACCLPDGGCVDELSPSECAAAAGVYQGDGTQCGLIDCPDPTGACCIASTGGCVV
                     LSEAGCAVVGAYWAGFGTTCVDLNQNGKADACEAGDCNCDGAVNVFDIDAFVLALTDP
                     AAYYATYPNCFISNADTNRDGTVNVFDIDPFVALLTGE"
BASE COUNT         5389 a         9251 c         9592 g         5249 t
ORIGIN      
        1 ggatcaattc cgccccaagc gccgccagcg ccggcacatc cgcaaccgcc gcttcccgca
       61 ctgtgaattt gctttgagtc atgtcggcaa ttctaacccg ttctagcgcc gagcaagacc
      121 ggggcacgcc gagacggctc cgaagcagaa aaaaacggcc ggcgccaggc cgaccgcgtt
      181 tggaggaggg tcttgtgctt ctgtgcaact ggttcgcgca ccgcctcgcc gtcgcgggca
      241 gggcgaaaaa ctctcagcgg tgatagtgct tgtggtgacc gccgctgtaa tgcttgtagt
      301 agccacgaac accgtagtag tacgggcgtg cgtggtaata ccgcgccggt gcgacgaccg
      361 gggcgtagta caccggcccg ccgtaaaccg ccgggtattc gtagacgacc gtggcgggcg
      421 cgggcacgac aaccggcgcc ggatagacga ccggagcgta ataagccgga cgaccccaac
      481 tgaaaccgaa cccgaagccc caaccagcct cggcgggagc ggccgcgagg cccaaaccta
      541 agagaccgac tgccgccaaa ccaatcgttc gcttgctcat ttgttcactc ccgatccgaa
      601 gatcgcttac tcgttcgatg ttcgctaacg agcttcacat cggtcagcat tactgacact
      661 atatcgtgcg ccaggtttgg caacggggcg cagtgccctg ggcacgccgc ggttcgcccg
      721 cttgtagccg ccctgctgcg cgatgcaaaa acccggcacc ggcatgagcg acgcgcgttt
      781 tatgagaccc gagaccccgc aaacgggata cgatgaaacg ctggccaagg cgcgtggaac
      841 acgcgatggt ctgtcgcgag gcgtgcccga atcacagagg aagacgagcc atgccgcaag
      901 aagtggtgtg cattaatggt cagatcgtgc cggccgacca ggccggcgtc agcgtctttg
      961 acgcgggctt catgcaaggc gtcggcttgt tcgaaacgat gcgcgcctat ggcggccgcg
     1021 tcttccggct cgagcgccac ctggaccggc tggcgaactc ggcccgggag ctcggctgga
     1081 cgctcatacc ggatcacgaa cagatgatcg attgtgtcaa cgcggtactg gctcagttcg
     1141 ccggccagga cgcccgcgtg cgactgacgg taacgaccgg ctcgctgcgc gccgcggaag
     1201 ccggagctgt gcggctgacc atggtggcta cggcgagccc cggcggaaaa tacgcggaca
     1261 cgttctacga acagggggtc gaggccgtca tttcacgcta ccgccaaagc cgctttgacc
     1321 cgctcgccgg ctataagacc accagctact tcgggcggtt ggcggccctg cgcgaggcgc
     1381 acggcaaaga cgccctggaa gcgatatggc tgaacgacca agctcttgtc gcggagggcg
     1441 ccatcagcaa cgttttcgca gtacgcgaag gccgactggt cacgccgccg ctggccacgc
     1501 ccattctgcc gggcatcacg cgggccacgg tgattgagtt ggccgcggag gctggcatag
     1561 cggtcgatga aaacgatctg acggtcgagc agttgaagtc gtcggccgag gtctttctca
     1621 ccaacagcat gatggaggtc atgccagtcg tgcggatcga ccgcgatgcg gtgctccagg
     1681 ggcggccggg cgaggtcaca cggcggctgg ccgcggctta ttctgaactg gtagctcggg
     1741 agtgcggaag atgagcagag cgcaaacacc tcgtgaagtg cggaaagcgg gcgcagccga
     1801 aaccgccgca gtcacggtcg ccacgctggc atcggcgctg aacgaaatcg cgcccccgta
     1861 cctggccgcc gaatgggaca atgtgggctt gctgaccggc cgtccggagt ggccggttac
     1921 gcgcgtcctg ctggcgatcg atctgacgga tgcggtggcg cgcgaggcgc tcgagaccgg
     1981 cgctagcgcg ctgattcttt accatccgcc gatcttcaag gggattcgca atatcacgcc
     2041 gcaagccgag tgtccgacgg cgctattgcc ggaccttttg gcggcgcgca tttcgctgct
     2101 ggcggttcat acggcgctgg acgcggcggt tggcggcacg aacgacgttt tgttggattt
     2161 cttcgagccg gtttcgcgcc gcccgctcga gttgcacatt gaggaacggc gcacgtacaa
     2221 gctggtcgtg ttcgtcttgc ccaacgaggt ggatgcgctg cgccgggccc tctctgacgc
     2281 tggagcgggc gtgatcgggc actacagcga gtgcagctac gccctggccg ggcaaggcac
     2341 gtttcgcggc gacgagacca cgcgcccgac cgtcggtgag aagctgaggc tggagacggt
     2401 ggacgaaacg cggctggaaa tggtcgtgcc gcgggcccgg ctgggcgagg tggtgcgcgc
     2461 cctgtacgcc acgcattcat acgaggagcc ggcgttcgac ctttacccgc tgcacgagac
     2521 gcccggacga gccgccgtcg gcatgggccg ggtcggcatt ctgccgcggc ccgaacgcgg
     2581 cgacgtgctg ctgggcaagc tgcgacggca cgtggacctc tccgcggcgc tggtcgtcgg
     2641 ccggctcaag cgttcgtttc gcagcgtaac ggccgcggcg ggcgcgttcg gcgtgcgggc
     2701 gttcgcggac accgactcac tggtcctcac gggcgagttc aagcaccacg acgctctgga
     2761 gctgcagaag cgcggcgtga cggccgtaca cctggggcac tacgccagcg agcggccggc
     2821 gctggagtcg ttgcgcgcca ggctgagcga gcgcgtggcg gaggtgacgt tcgaaacggc
     2881 ccgggcagac aaatcgccgc tatcgccggt tcgtctggag tgacgtttgc cgaggcgcga
     2941 gcccggtagc gatgcgggac ccaccgggta tgctagaatg ccgctagaat gatgtgagat
     3001 tcgggccgaa gccccgaacg tttgagtcag tttcccagac gccagaacat gaacgctgag
     3061 ccacgcattc caattgacgc cagcaagatt gcggcatttt gtcgcaagtg gcgcgttacg
     3121 gagtttgcac tgttcggttc ggtcgtgcgc gacgactttc gcgccgacag cgacgtggac
     3181 gtcctaagct ttgagcgaga tgcgccctgg agcctgtggg acctgcttgc catgcgggat
     3241 gagctagggg aactcttcgg gcggcgggtt gatctggttg aacgggaagc gcttcgaaat
     3301 cccttccgcc ggaaagagat tctttccact cggcaggtga ttcatgcggc ctgaagcggc
     3361 cgacgccggc tgcaaatagt gccgcgcgct gtgacaacgg actgtgacaa tggggacgca
     3421 gacagcggtg gtgataaagt gataaggtga tagagtgata aaggggggct gtgcgctgtc
     3481 ctttatcact ctatcacctt atcacgtcat cgcacactgc ttgcagtcgg tgtaattgat
     3541 ccagtcgaac gctggcgggg gctatttggc ttccagccag tttgcgccgc tggcgatgtc
     3601 cacttttagc gggaccgtta gcggcagagc gtgagtcatt atgtcgcgaa tgatggccga
     3661 catgcgctcc acctcttgcg ccggtgtttc gaagacgagt tcgtcgtgca cttggagcag
     3721 catatgcagc ggtaagcgct cgcttttgat gcgctggtgc agccggatca ttgcggtctt
     3781 gatcaggtcg gcggccgagc cctggatgac cgtgtttact gccaggcgct cggcctgggc
     3841 gcgggcggta ttgttgcgcg agttgatgtt tggtatgggg cggcggcggc cctggatggt
     3901 ccggacgtat tcgttgcggc gggcttcggc gacgcactgg cgaatgaacg cttgaatgcg
     3961 cggatagcgg cggaagtagg cgtcgatgaa ttccttggct tcggtgcggc tcatgccggt
     4021 cgtctgcgcc aggccgaacg gcccctggcc gtaaatgatg ccgaagttta cggccttggc
     4081 ccgggcgcgc atttctcttg tgacctcggc caacggcacg ccgttgacct gggcggcgac
     4141 gaaggcgtga atgtcgcggt cttccgtgaa agcctcgatc agcccctcgt cctggcaaaa
     4201 gtgggccagg acgcgcagct cgatttgtga atagtcggcc acgatcagcc gcccttgcgc
     4261 actgcgcggg atgaacgcgc ggcggatttc gcggcccagc tcagtgcgaa tgggaatgtt
     4321 ctgaagattc ggatcgctgc tggacaggcg gccggtgatg gcggccgtct gactgaaaca
     4381 ggtatgcacg cgtccggtgc ggcgcgagcg ggccagcggc agcgcatcca cgtacgtgcc
     4441 gcggagcttt tgcagctcgc ggtattccag caacggttta agcatggcgt ggccggtctc
     4501 gcgctgtagg actgcgagcg tgtcggcgtc ggtcgagcgg gcggtcttgg tgacgcgcac
     4561 cacgcgcagc ttcagctcat caaacaggat ttcggccaac tgccggggcg aatcgagatt
     4621 gaagcgccgt ccggcgtgtc ggcagacttc gtcgcagagg gcctcggcac gctcggccat
     4681 ttgccggctc atggccgcca ggaaattcac gtcaatattg atgccctcca gctccatgtc
     4741 ggtcaggacg gataccagcg gcatctcagt ttcatagaac aggtcggcca cgccggtggc
     4801 ctggattttc ggttccagca gcatgcgcag ccgccaggtg tagtcggcgt cctcggccgc
     4861 gtattcggtg atgcgcgaga gcggcacctg gtccattgag agctggtcgc ggcctttgcc
     4921 gatcagatcg gtgatcggga tcatctcatg cccgagcagg ccgaacacca gcgagttcag
     4981 accgtaggag gcgcgtgcgg gatcgacgac gaacgcggcg atcatggtgt cgaacagggg
     5041 cccgttcagc ggcaggccgg ccaggcgcag gacgagcgag tcgtacttga gattgtggcc
     5101 gaccttgcga atcttgggat cggccagaat cgggccgagg cgttggcgga cgaggtcgag
     5161 cggcagcgcg gcgccaaagg cgcagcgcac ggggacgtag tacgcctggc cgggtttcca
     5221 ggcgaaggag gtgccgacca gttcggcgtc gatggggttg atggcggtag tttcggtatc
     5281 gagcgcgaat tcaggctggc gggccagttc ggcggcaagt tcgtccaggt tctcgggtgt
     5341 attgaccagg cggtagtcgc cgccttcagg ctgtgacagc tccgccacct cgggccgcga
     5401 gatcgcagga atagcgggcg ccggcgttgc ggacgactgc tcgaaaaggc tggacgcatg
     5461 cgcgggggac tgcggctggg ggttggtgcc gacagcgacg tctggcgccg ctggggttgt
     5521 tgcattcgca gtgtcgttct tagcgcgcgc cgcggccggc aactggtcca gcagccggcg
     5581 aaaaccgagt tcgcggaaga tcggctcggc ggcggaccag ttaattcgct cgcagcgggc
     5641 ctgttccagg tcgaactcga gcggcacgtc gcgccgcagg gtcatcagtt ggcgcgagat
     5701 ttccaagcgc ggcgcgaacg ccagcacgtt ctcgcgctgc ttgggcgtca gttcgtgtgc
     5761 gtgagcgatg acttcctccg ccgagccgta tttctgaagg agcttggcgg ccgtcttcgg
     5821 gccgatgccg gcaacgcctg aaacgttgtc cacgtcatcg ccgatgagca cctgggcgtc
     5881 gatggcctgc tccggcgtcc agcccttcag ctcgggcagg cgttcggccg tgatgacctc
     5941 gtctttaagc gggtcataga gggcgacgtg cggcgagagg atctggtcga ggtccttatc
     6001 gcgactgacg gcgtaaagcg tcaggtcggg cccggccaga cgggcggcca gcgtggcgat
     6061 gatgtcgtcg gcctcaaagc cgggcacgcg caggatcggg attccgacgg cctggacgat
     6121 actgatgatg cgatccatct gcaccggcag gtcttcggga gagagctgac gctgggcctt
     6181 gtattcgggg taaatctgca cgcggaagac cgtgcgatcg tcgacgtcca gggccatggc
     6241 gaggtagtcg gggcgccggt tgcgcagcag gttcagcagc atctgtgaga acacgtgcgt
     6301 ggcgcgcgtc ggctcgccac tgggcgaact caggttgccg aagggcgcgt agtaggcgcg
     6361 gtagatttgc gagtggccgt cgatgacgta tagcgttctc gccatgtcaa aatggtaagc
     6421 gcatcctgat tctattgcag caacgctatc gcgggtgtca gcgcaatagc ccggctgcaa
     6481 ggggtctgct ggtgcgctct gtgtgaatcg acgtcgatgt cagccggaac cagcacgcac
     6541 gccacgaagt gcagcgtgcc tacaccacta gcgcagacgc gaggcgattt ggggcccctt
     6601 ctgcggcatg ccttgccgat caagcttgcg gttcggaatc gccattttcg cctgcctcgt
     6661 cgctctcagc cggggcttca ggttcaacat catcaggctc gcgcacctca ccgttgccgg
     6721 ccgcagcggc gttcgcggcg ggcgtctgag taaggatgtc agcgctcttg tcggcccctt
     6781 cgccggccgg gctgagaccg ttaatggcgt tctcttcttt ttcgctgacc acgcgggcaa
     6841 tcgctacgac gcggtcgtcc tcgtcgacgc ggatcagccg cacgccctgc gtggcgcggc
     6901 cgatttcgcg gagatcagac atgggcatgc gcagcagcgt gcccttggcg gtgatgagca
     6961 tcaggtcgtt ctcgtccgtg accgcccgca tggcgatcac cgggccgttg cgctcggtgg
     7021 tcctgatatt gatgacgccc ttgctgccgc ggtgcgtgag gcggtattcc tcgatgtcgg
     7081 tgcgcttgcc gtagccgttc tcgcacacgg tcagcaggct catgcccggc tcgatgacca
     7141 ccatgtcgat gacgcggtcg tcgccggtca gcgcgatgcc gcgcacgccg cgggccgagc
     7201 ggcccatggg gcgcacctca tcctcgttga agcggcaggc catgccgttg cgcgtaccga
     7261 gcacgatctg ctgcgccccg ttggacagcg agaccttgat caggctgtcg tccaagtcaa
     7321 gcgacatggc gatgatgccg ctgggacgcg ggttggagaa cgcccgcagc ggcgtcttct
     7381 tcacgatgcc cttctcggtc gcgaagaacg cgtactgctc ctcgaactgc gacaccgaca
     7441 gcacggccac gtgccgctcg ttgggctgaa gcgtgagcac attggccagc gcccgcccgc
     7501 ggctggtgcg ctgcatgctc ggaatgtcgt agacgcgcag ccagtacacg cgtccgcgat
     7561 tcgtgaagaa cagcaggtaa tcgtgggtcg aggcgacgcg caggtgctcg atgaaatcgc
     7621 cgtcgcgggc ctccgtgccg cggatgccct tgccgccgcg accctggctg cgataggtgt
     7681 cgatcggcac gcgcttgatg tagccctcgt gcgagatcgt gaccaccacg tcttcctgcg
     7741 cgatcagctc gtccagttcc agcgtgccca cgtcgccccc gatctgcgtg cgccgctgct
     7801 cgccgagctt ctctttcagc tcgcgcatgt cctcacagat gatgtccagg acgcgccgct
     7861 cgtcgctcaa gatcagctcg taatccgcga tttcctcgac cagcttcgtg tactccgccg
     7921 caagctgctc gatctccagc ccggtcagcc ggcgaagctg catggccaga atggcgtcgg
     7981 cctgagtgtg cgtcaggcgc tgatccgcag ccgtcgcctt ggcgacaaac accgccggta
     8041 gcagcgcccg cacggtcgcc gcctccgaca gccgcaggtt cttctccatc agccgctggc
     8101 gcgccacgtc ggcgttcggc gattcgcgaa tgaggcggat gatttcgtcg atgtccgcta
     8161 cggccagaat gaggccttcg agtacgtgcg cccgctggcg cgctttgcgc agcaggaact
     8221 gcgtcctgcg gcggatcact tccacgcgat gccgcacgaa atgctcgatt agctgcttga
     8281 ggccgagtgt ccgcggcacg cggttcacga gcacgacatt gatcgcgtga aggtgcgact
     8341 ccagtggcgt gtactgccag agctggttaa tcgtgacctg gacgttggcg tccttcttga
     8401 gcggaatgac cagccgcacc aattgctcgc ggccggactc gtcgcgcagg tcttcgatgc
     8461 ccttgatcaa cccgctttcg acggcctcgt tgacgcgttc cttgatggtc ggcaacagca
     8521 cgccgtacgg gatttcgtcg acgacgatct ggatgcggcc gttcttgagt tcctcggtgt
     8581 gcaggacgcc gcggactttg atggagccgc gcccggtttc gtaggcctcc ttgatgccgg
     8641 ccgtgccgca aatcacgccg ccggtgggga agtcgggccc ggggacgatc ttcatcagct
     8701 catcaatcgt gatattggga ttgtcgatgc aggcgaccac ggcgtcgcag atttcgccca
     8761 ggttgtgcgg cagcaggcgg gtcgccatgc cgacggcaat gccctccgag ccgttgacca
     8821 gcagattggg gaacttgcca ggcaggacga ccggctcttt ccgcgttccg tcgtagttgt
     8881 cctcgaaatc gaccgtttcc ttgtcgaggt cttcgagcat cagctcggcc ggcgtcatca
     8941 ggcgcgcttc ggtgtagcgc atggccgcgg gcgggtcgcc gtcaattgag ccgaagtttc
     9001 cttgcggatc tacgagcagg tgccgcatgc tccattcctg gcccatgcgg accagggtgg
     9061 ggtaaatgac gccctcgccg tgcgggtggt agttaccgct ggtgtcgccg cagattttcg
     9121 cgcatttgcg gtgcttggcg cgcgggtgca ggttgaggtc gttcatggcg accaggatgc
     9181 ggcgttgcga gggcttgagg ccatcgcgca cgtcgggcag agcccgggcc atgatgacgc
     9241 tcatggcgta ggtaaggtaa gactcctgca tctcctgcga gatgggcgtc gggatacttt
     9301 gcggggtgat tggcgccccg gcggttggcg tcgcgctcat agttctccgc tatggtcgcc
     9361 agaggcgact aattcggctt cgaagcagta acagcggctg caaagtccgc ccctcgttcg
     9421 cgcccttgct cgctgccgga ggcacgaggc tgctcccgat tcccgggccc ttccggcgcg
     9481 cttggaactg cttctcttaa taatatttta gccgattacc gcggctttcg cagcaagcgc
     9541 gagctaagtt ttccggcctc caaactcggc gatcagcggc gtcacggcct gccgcgaacc
     9601 tcgcgcccgg cgttaattcg gatttccaat gttcggtact tgttcgtgtt gcggacttag
     9661 agtacactac tcagcttcag ggtacgggac ggaggtcgcc ccgtgttgcg gtcttagttt
     9721 caaggagatg gttcatgaag cgtagctatc ggtggatgtg gagtgggtgc gcgctgatgg
     9781 cggcagtggc gctggccgtt cagggcttca cgcaggagaa gaagcccgac caaccggcgc
     9841 agccggaaac gaaaccgccg caggttcagc cggagaaaaa accgggcgct ccggctccca
     9901 ccgagatgac accggaagag atgatgaagc tgtggcagga gttgaacccg ctgacgccgc
     9961 agcaccaaga aatggccaag gcggtcggca agtacgacgt gctggtccgt taccggatgg
    10021 cgccggatgc gcccatgcgg gaatcaaagg gcaaggccga gtttaagctg atcctcgacg
    10081 ggcgtttcat cgaacagacg tactccggtg acatggaggg cgtgcccttc acgggcttgg
    10141 gaatcgaggg ctacgaccac ttgaaaaagc agttccagac ggtgtggatg gacagcatga
    10201 gcaacatgat gatgcacacc accggtacgg ccagcccgga cggcaaggtg atcacctaca
    10261 ccggcaagat ggacgatccg atgaccggcc agaaggacaa acccatcaag atggtcctgc
    10321 gcaaaatcag cgacgataaa acggtcttcg agatgtacga tacgttgccg gacggcacgg
    10381 aattcaagtc gatggagatg gagtacacgc ggcagaaata gcccgtggcg gcccgggatt
    10441 tgcctgaacg gggcgggccc gcggcctgcc ccgttctcgt gcgccgccag cggcgcgagc
    10501 ggaggatcat ccggggcgtt tccgatccaa ctcggctaca attgacgtat aaagtggcgg
    10561 tttggcccga agccggacgg ggcggaaact cgcgaatgcc caggacacgc aagacaggta
    10621 cgggagctgc actcagcggc cgcattacgg ccacaaatgc ccgccgcgcg tgcggtcgga
    10681 gtacgctcgc gccgcccggt cagccaggct cttccgatcg tgcaacgttt tccgcggaag
    10741 ctgcagtcca gccgaacggc gaggtaaact ggctggtcgt tcgaggagcg gcgcacaaca
    10801 acctgaagaa gatcgacgtt tcattcccgc tggggcggtt cacctgtgtc acgggcgtct
    10861 cgggctcggg caagagctct ttggtcaacg acatcctcta cgccgccctc gcacgcgagc
    10921 tgaacaacgc caccacgctc gtgcctggcc gttatagccg gatcgacggc ctggggcatc
    10981 tcgacaaggt gatcgccatt gaccagtcgc ccatcgggcg cacgccgcgc agcaatcccg
    11041 gcacgtatat caaggtcttc gacgagatcc gcagcctttt cacgaaactg ccggacgcga
    11101 agctgcgcgg ctacaagccg gggcgcttca gcttcaacgt tccggcccag cgcggcggcg
    11161 ggcgctgtga agcctgcgcg ggcaacggct ccaaccgcat cgagatggac ttcctggcgg
    11221 acgtgtgggt cacgtgcccg gtttgcggcg ggcaacgctt caaccgcgag acgctgcagg
    11281 tccagtacaa aggcaagtcg atcgccgacg tgctcgaaat ggacgtgcag caggccatga
    11341 cgcattttga gaacgtgccg cgcattcggg agatgctcca gacgctgcac gacgtcggcc
    11401 tggattacat caagatcggc cagtcgagca ccaccctttc cggcggcgaa gcgcaacgta
    11461 tcaagctggc ccgcgaactg gtgaagaagg cgacgggccg aacgctgtac gtcctcgacg
    11521 agccgacgac cggcctgcac ttcgacgaca ttcgcaagct gctgggcgtg ttgcacggct
    11581 ttgtggacgc cggcaacacg gtggttgtca tcgagcacaa cctggacgtc atcaagaccg
    11641 cggactggat cattgatctc ggaccggagg gcggcgaaca aggcggctgg atcgttgcgg
    11701 agggcacgcc cgaggaggtc gcggcgtgcc aggcatcatt tactggtcag gcgctgcgcg
    11761 aagtgctgag cagtgggaat ggattgtcca agcgcggaaa agtccgccgg agcgcagcac
    11821 cttcccgcct gccctctttc tcgctttccg acgcctgcga cggcgaggcg acggccgcta
    11881 gcggtctgac caccgacgcc atcaccgtgg ccggggcgcg gcagcacaac ctgaaagacc
    11941 tcgatgtctc cttcccgcgc gagcgcatca ccgtgtgcac cggcgtgtcc ggctcgggca
    12001 agacgagctt cgcgatcgat acggttttca ctgagggtta ccgccggtac gtggaatcgc
    12061 tttcggccta tgcccggcaa tttctggggc aaatggcgaa gccgcgcgtc gatcacgtgc
    12121 acgggctgtc gccggccatc tgcatcgagc agaaggcggc cagcaagagt ccgcgctcga
    12181 ccgtggggac gatcaccgag atatatgact acatgcgcgt gctgtgggcg cgcatcggca
    12241 cgccctactg cccgcgttgc caggtgccga tcggctcgca gacagtcgat gaaatcgtcg
    12301 cacgtgtcat ggccctggag ccgggcgcgc cgatcatgat tctggccccg gtcgcgctgg
    12361 gcgacggcga atcgtacgcg accatgttca accggctgaa gaattcgggc tacacgcgcg
    12421 tccgcattga cggccagaca atgttgactt cgcacgcggg catgctcgac gcgcggcgca
    12481 agcaccgcgt cgaagtggtc gtcgatcgca cgacggtgcg ggcccgcgcc cgcgggcgca
    12541 tcgccgagag tatcgagcac gcactggcgc tggggaacgg cctggtagtt gtggtcaagg
    12601 aggcggtcga gacagtacgc gaagagaggg atcgagggat cgagggatca agggatcaag
    12661 agaccgatgg tgaggctccg gctcgccaat ccgagccggg cgagtcggcg cccgaagcgc
    12721 cggccggtgt gccactgctt gaaagcgcag cttcaagcag tgccgctccc gacgcagcag
    12781 tcaacacctc ggagtccggt gacgaagcgc ccgactccgc cctgatcacc gtcaacaccc
    12841 cggcgcgcga attcatgttc tcgcagaagc tggcctgcat gcgctgtggc acgagctaca
    12901 ccgaactcgg ccccaaccag ttcagcttca acagccagct cggctggtgc gagacgtgcg
    12961 agggattggg cgtccagcgc ggggccccgg cggccgaaat catcaagcat cccaatcgct
    13021 cggtcttcaa cggggccatc gccggctggg ggcgcgtcaa tcccaaaacg ccctttggca
    13081 agatgttggg cgcattgtgt gatcacctgg gcatagcccg cgacgcgccg ctgaccaagt
    13141 ggaccgccga gcagcgccgc atcctgctct tcggctcgcc cgaggggcaa tgggtcgacg
    13201 gcggccgcgc ctacgacggc ctgcgcttcc gctggcgcgg cctctttccc acgattgatg
    13261 ccgccacgcg caatagctgg atgctgcgct accgcctgca aaacgtggtc accgacatcc
    13321 cgtgcgtggc ctgtcacggc gggcggctgc ggcccgacgc cgccgcggtg cgcctgggcc
    13381 cgcacgcaga cgagggggcg caaacggatg accagtaccc gccgcgtcag ggtgatcagc
    13441 actccgcaga ggcccgcgcc ggcggtcgca ccatcgttga ggtttgcgaa ctgccgctga
    13501 ctgaggcggc gcggttcttc gagacgcttg aacgcgaact ggatgcacat cggcggcgca
    13561 tcgcgggcga agtgctgaag gagattcgca atcggctcca gttcctgctg gacgtcggtt
    13621 tggagtacct gacgctacac cgcgccgccc cgacgctttc cggcggcgag gcgcagcgca
    13681 tccgcctggc cagccagatc ggcagcgggc tttcgggcgt gttgtacgtg ctcgacgagc
    13741 cgaccattgg cttgcaccca cgcgacaacc tgcggctgat ccgggccttg caaaagctcc
    13801 gcgacctggg caacacactg ctaatggtcg agcacgatcg cgaggtcatc cgccacgccg
    13861 accgcctgct cgacttcggc ccgcgcgccg gcgccgccgg cggccacatc gtggcccagg
    13921 gcaccccggc agacttccta acgcccagcg cccagaacat accgagtgtg ccgagtgccg
    13981 gggtgccatg cccgagctct aagcgcgggc atgcctccgt gaatacgccg caacctcaac
    14041 aaaacgcctc cgccccaccg gacgccaacc aattccacga atcactcacc cgcgcatacc
    14101 tgtccggaca gtgtgccata cccgtcccca ccaatcggcg gccggtcaag atcgaactgg
    14161 cgcgaccaaa ggcggacgga ccgcaccatg tacaaaagac ccgccgcaag tccaacacca
    14221 tgggcacggc gcccgcctcc acgcaagccg gctccgttct gctggtgcgc ggcgcacggc
    14281 agaacaatct gaagaacatc gacgtcccca ttccgctggg ccgcttcacg tgcgtcaccg
    14341 gcgtctcggg ctcgggcaag agttccctgg tcaacgacat tctctggccc gcgctggcca
    14401 taaagctgca gcgcgccacg atgacggccg gcgagcacga cgagatcgaa ggcgttgaaa
    14461 acctcgacaa agtcatcaac gtcgaccaat cgcccatcgg ctcgaccccc tccagcaacc
    14521 cggccaccta cacgggcgcg ttcgactgga tccgcgagct gttcgcgcgc ctgcccgaca
    14581 gcaaagttcg tggctacaac gccaaccgct tcagcttcaa ccgccccggc ggccgttgcg
    14641 aagcctgcga aggttacggg cagaagtgca tcgaaatgca cttcatggcg gacgtatggg
    14701 tcgattgcga aacgtgtcac ggccgccgct acaaccccga aacgctggaa gtgaagttca
    14761 aaggcaagtc gatcgccgac gtgctcgaaa tgcgggtcgc cgaggcgctg gagctgttcg
    14821 ccaacgtgcc caaggtccgc cgcatgcttc agacgctggc ggacgtcggg ctggactatg
    14881 tccaactcgg ccagccggcg cccacgctct caggcggcga ggcgcagcgc gtcaagctgg
    14941 ccgccgaact cggcaaaccg gacaccggcc gcacgttcta cattctcgat gaaccgacga
    15001 ccgggctgca cttcgacgac gtacgcaagc tgctgagcgt cattcaccgg ctggtcgacc
    15061 tgggcaacac ggtgctggtg gtcgagcaca acctggacgt aatcaagtgt gcggattgga
    15121 tcatcgatct cggcccggag gccggcgaag gcggcggatg gattgtcgcg atgggcaccc
    15181 cggaggaagt cgcgagaaaa tcagaactga gcagtcagaa ctcagtcaga gggcggaaat
    15241 cagcggcgct cagcaaacgc gggaagcagc cgctcggccg cgaaactcgc aacttcctac
    15301 ttcccccttc tgacttcgtc tgccacaccg cggccgcgct ggcgccggtg ctcgcgctcg
    15361 gcccgtatga gccgcgcacg ccttacgacc ccaaggccca tgcccggcgc gaaatggagg
    15421 tcgccaaggc cggcttcggg cgcctcgggc gcgaggcgcg cctgccctgg cagatcgatg
    15481 ggcgcaagtg gcacctcgag cagcgcacca gccgcgaggg caagccgaag cattgggagg
    15541 ccgccgcgct ggagtacgtg atcgacctcg tgcaggaaaa cggcggcttt gccccgacaa
    15601 attggaatga acgcgccagc gtcgagatta ccgctgacgg cgcggaaaac tggttcctgc
    15661 acgccctcac tggcgacgaa tggctgctgc agttgtgctt ccttgtgccg ccgggcacgt
    15721 ttgagcggcg cgccctcgaa cggcagctcg gcctgaaaac gctggatgag cgcggcgacc
    15781 tggaaaccta cggcgactgg tcgcgcgttg atattcgccc gcgcctgcgg ggcggcgagg
    15841 ctgtggtcat ctacgtacac gacaagcagg aaatcgacac accgggcttt cgcaagttca
    15901 ttcgtgacgc agcccgggcg tacctcgaat cacagcggcg cggcggccca aaacagaaat
    15961 gagcacaaga atgcgcggct gtaacgtcac agggctgttg agatgattga ggcgatttgc
    16021 cggcgggcgt tctttcagtt tgagttcccg attcattacc gggcgcgtac gccggtgatt
    16081 gatggcgacg tctccaaatg gggcccgcac tttttggttc cggcgctggt ggaaatggag
    16141 gaccagccgc ctttcgcgga cgtgtactgc gcttggaacg ccgatcattt catggtcgcg
    16201 tttgacgttc cggaaagatg gggtccgcta cgctgcgacc agacccaatg gtggaaactg
    16261 gacggcgtcc gcatttgcat cgacacgcgc gacacacgtg acgcgaagcg agccacgcgc
    16321 ttctgccatt tcttttacgt cctgcccacc ggcggcggcc cgcgccgtaa tcagccggtc
    16381 atcggcctgc accgcatgag tcgcgccaag gagccgccgc ccgcggtcga cctggcgcaa
    16441 attcagctcg ccgcccgcac cgcccgccgc ggctggtcag ttgaagtcgc gatacccgca
    16501 agcagtctca gcggctggaa cccgtctgag cacccgcgca tcggattctt ctacaaggtg
    16561 aaagacacgc aacttggcag ccagcatctg acggttgacg atgaactggg ctggaacgcc
    16621 gacccgagca cgtgggcgac gggcgtgctg gtgaagtgaa aagtcgcctc attatccact
    16681 tggccgagtc tcatttatac caattcacca gctagttagt cgggattaag aaacccgagt
    16741 tattgaacca tcattcgggc cttcggcgaa cccagccgtt ggggacggtc cctgtccgct
    16801 tcggttcgca atggttccgt ttgaactgga gactgagatg atgaaacgtt gctttgacat
    16861 cggtgtcgtg gttgtcgcac tgttagccgt tccgcttgcg ctggctcaaa cgggcagcgc
    16921 gggacagtcc ggcggcagcc agtggaccac aggtcagtac ggccagatgg gcgctgagcc
    16981 ggggcacccc ggccagtacg gtcacaccgg cgggcaatac ggttacaccg gcgggcagta
    17041 cggcgcccaa taccaaccgg gcgctatgta tggcggccag atgggggcct atcaaggcca
    17101 gtcgggcgca tacggcagcc aatacgtccc cgatttcggc tacgggcagt ttggcagcta
    17161 cggcagcccg tacatcccag aatggagcta tggcgggggc cagcgcgggt attggcaggg
    17221 cggcatgtac ggacagaccg gccagtatgg ccaatttcgt gggacgcaat acagcagccc
    17281 ctatacgtac accggttatc ctcagtatca gggaatgcaa taccattctg gccaatctgg
    17341 ccaaggcttt cgtggcggct attatccggg ctatccgagc ggccagccga gcggcggata
    17401 ctccgggcag cccgactggt atagctggca gaaccgctac aaccagccgg actggagtca
    17461 acagggcgtc tggagacaac gcagtcaaca gccctacggc tactccgggt acggacagca
    17521 ggactattac cgcggcagct attacccgag cagcgcttgg cccggctacg gaaacccggg
    17581 ctggcataat cagcccgctt cctatactca atgggactgc cgcagcatgc catgcccgca
    17641 gggttatggc acggtcgggc atatgcgttg tgataatctg tacggcactc cctgctacaa
    17701 cctgattaac ccgtacaacg gttcggttgc gcacgaggct ttcgagcggt aagcgtcacc
    17761 gcgggcctca agccgttttc agccaagcga gaacgccgcc ggcgcggcct gggtcgcgta
    17821 atggcggcgt tttgccttgc gctgccggcg ccgctgggtt ttttgcctgg tcccgggccg
    17881 tgaaaccggc ggaaggtgcc gcgccggtta tgacgcgcag tttgtcgatg ctggaagggc
    17941 gaacggggcg acccataaaa gctggtccgc cggcagagaa accgcgcccg ggcacgcgtg
    18001 aaaggcaacc atcggccgcg ccggcaggtt ttcattgcct ttgcattcgg ccgcggtatg
    18061 gttgttacgg agcgtggagg aggcatgggc aaagactact tcgccattct cggactgacg
    18121 ccgggccgct actccgggcc ggaaatcgcc cgccgctttc acagcgagcg cgcccggctg
    18181 ctggcacagc tcaacgagcc ggccctgcac gatgcggtgt gccaacgcct ggacgaactg
    18241 cacctggcct acgccgctct gcacgaccct cagggccagg agcgcgccct gcgcctgcgc
    18301 ggtgctgccg ccgaccagat cgcggccctc aaaatgctga tcgccgcgtc gctggaggat
    18361 ggcttgctgc gctattcccg ccgccagcac atactgcaag aggcccggca actcggcctg
    18421 tccgacttcc aggcgcatct gctgatcgca gaggtgcagt tcgcagatga cgattttgcg
    18481 gccgactggg tgagagccgg cgaactcccc gcggtcactc gcccagtgga gagacggcgc
    18541 ggagcggggc ggcgtcttgt agcggccggc gtgctggcgc tgggaatctt cgtggccttg
    18601 gtgcgctggt tcgcggccta actcattcca agccgtagct cttcagcatg tcctttgaca
    18661 gcatgaccag aagcatctgc cccacgtcga aggcgaagtg cgccacgatg gcagccagca
    18721 ggctgcgcga atagatgaag attagcgcga aaaccaccgc cacacccgag atttggagga
    18781 tccccaggat gccctgctga aagtggagaa ccgcgaatat tacggtcgag atcaaaaccg
    18841 ccgaccacca atagcccgtg atgcgacgca agtacggcag catcagcccg cggaagagaa
    18901 tctcctcgct gacggccacg gggatcatca gtatgatcac ttttccgaga gtatcgggca
    18961 gcagctctat cagatcctgg cgcttggtaa ggtcggattg gagcccgggg aacagttgta
    19021 acacggcgct gatcacgatc acgctaaata tcatccagcc gtacgccacc cccaggccgg
    19081 cggcgcccca caacaactgt tgccccggca tgtgtgcttg cactccgaac gcgcatgacg
    19141 ggaggttgcg gcgccatagc agaaaagaga gcagcccaat gctcaggatc acctggaaca
    19201 gttgctggct catcatcatg cggaaagtgg gctgatcggg cagctctcgc ccggtcgccg
    19261 ccatccacgt gcctaccacg atactcggtc cccccagcgc gaccgcggca accagcagca
    19321 agagcaagaa atcgacaacg gccgcgcgga gaggaagccc cggcagcgtc aggcaggtcg
    19381 cggatgcggg cgtctgggcg gcggacactg cgcgggcgcg cacggcgccg ccaggcaagc
    19441 ggcctgggag agcgccgggc ggcatttcca ccggccgtgc aaagacgacc tccggttgcg
    19501 gtgcttcggg cgaaaggggc gaatcgctca cgaattcctc attagccggc gcgaaccgcg
    19561 ctggatccga gcgtaacgcg cgagccaggg caatccgcac tcaccggggt gacacgcctg
    19621 acgcgccatg gcgagccggc ttcgctcccg ttcttggcat ttttgtcatt ctgcatcgca
    19681 taatccccga cacgccccgg ctcggctccg ttggcgcggc gcgaatgatc atgataatag
    19741 acgaccgcgc cgaacgcggc gcagacgttc aggaaattgc atagcctacc acatactaca
    19801 caacgctgga ttgatggatg agcggtaatg caggcaacct atccggccca cgcggcggat
    19861 tgaggccggg ggctgggctc acgcgcggct ggcattggga acgcgtcgga gaattgctgg
    19921 tctcgaatcc ggtccgttgc aaacgcgacc agtgctatct ggcgcgcggt accgccgcag
    19981 atggaatgcg gccggtgttg gctgagttcg cgatcaggtt cctgcaggac gggcgcgaac
    20041 tcgcgcagcg caggctggcg ctgcacgcca ggaacggcca cgacggagag ttgctgggtt
    20101 ggattcagac gcccgaaaac gcaacgcatc tgcaaatact cgtgccgcgg gccgcgtcag
    20161 ccaacgtcct gggcgaagtg acgtttcacg cggtcgcgga acgcgatccg aaatgtcacc
    20221 cgctggccaa cgtgccgcgc tggaacacct atcgcccgcc gtttcctatc cagcgcgtgc
    20281 tgttgcctgc ttccctggcc gaacttgcca gcgctgtccc ggagctggcc gtcactcacg
    20341 tgcgcccgcg ctcgcgcggc gcactggccc atcaggccaa tcaggccgcg tgcgtgctcg
    20401 atccagcctg ggtcgacggc ctgggcctga cctgggagga tttgctgcgc ctggccgacc
    20461 gctgttggct gctcgtggat ctcgaaaccg ccgggaaact cctgagccgg gcgggcgtgc
    20521 gcgggttgga gcttttcacc gatgaggcgc aacatggcct catgtcggcc cgggtcgaat
    20581 acgccgatgt gcagacgcgc ggcttcgcat tgcaggatgt atttccttac ggcgccctga
    20641 cgccggccgg cggattttgc gtacgcgcgt tgcgagccaa ccgcgcctgg cggcgtctgg
    20701 ctgacgaact ggcgctggcc acgctcttgg ccagcgaaac ccccagcccg cgccaaaccg
    20761 gtcacgtcct gtcggcggcc gccgcactcg ggcgcggcga attggtgatt accgatttgc
    20821 cctggctggt ggccggaagc atgggcccgc caatagcgcc aagattggcc caacacgctt
    20881 tgcgtatgca cctgggggcc cccgtcgaag acgacctgca atactggaat cgctgggacg
    20941 atagccagat cgtggtgcgc gatatctgcg atttggcacg ccgctatccg ccactccaaa
    21001 ccgttcgctg gacgccctcg cccgagggca acgcgtgtct cggagtgtat ttgcccgctc
    21061 agaaccgcgc ggcccgcgcc accaccgttc tgatccacac cggccgcatc gacaatctcg
    21121 acttgcacgg gggtctgccg ccggagccga tgatgatcat catgaagcaa ctggcgcgcg
    21181 aggcgcgcga ggcgaccgcc tgggcgcagc gctacctggc aaatgtcaac gtggtctggc
    21241 agttcgacac cgcagccggc ctgcgctacg ctgtgaatta cgcctcagcg gagtcctttg
    21301 acttcggtac tgtttactcg tctcacctac atccgttgaa cgggcgtaag gactgcaccc
    21361 cttcgagcgt gcggaacggg gccgcaacgc gtgacaatcc ggcgcgcggc gagagtgcgc
    21421 ttctgacaca aggcgagggg atttatggtg acggatcgct ggactatctg cgccagctca
    21481 tgtcgcatgt ccgcgggctc atcgaagcgc aagcggtgtg acagtgacgc gtggcgcgtt
    21541 ctcggcaacg acctttatcg cgatgctcgt aaaataaata agccccggac tggccaacgt
    21601 atctacgaag ccgggagatt atctccgggc catccggggc ttcagcatgc acgttaagaa
    21661 aatcgtgctg cttaaccaac tttcaaaaac acttgaaacg tgcttccgag cgcgttttac
    21721 tacttctctg atcgtagata tcggcaacta agacatgtag atcagcataa cggtggtgcg
    21781 ggcgacccga taagaattgc cgatcccgca ggcgatcctc acggacgact cgtcgcggct
    21841 ggctgcgcgc ggtcacgcaa gtcctgccac ggatccgcaa catggcacac ccgcccggtt
    21901 ttctcgacgt aatcctggtg gtaatcctcg gccggccaaa atgtcttggc cgcctcgacc
    21961 tgcgttacga tcttgcgccc gccgaacgcg cgggccgccg tcagctcggc gatgtacgcc
    22021 tccgcttcgc gccgctgctg ttcgttcgta taccagatgc ccgagcggta ttgggacccg
    22081 acatccggcc cttgccgatc cagctcagtc ggatcgtgca tcttgaagaa cgcctcgagc
    22141 agccgccggt aggtgatccg ctgagggtcg aaaaccacct tcacgctttc ggcatggccg
    22201 gtgcgcccgt tgcaaacctg cttatacgtc gggcggtcca cgtgcccctg catgtacccg
    22261 gcgacggcgt caattacgcc cgggccttgc tgaaagtagt gctcgatgcc ccagaagcag
    22321 ccgcccgcga aatatgccgt ctcacgactc gcatccgtgc tcggggcgcc cgccgactga
    22381 gtggccggcg gcgttgcggg ctcagaccca ggccgcgcgg ctggccggct ttcgggcggc
    22441 agcgcctcgc ttttggtgta aaacttgagc gacaccgaat tgagacagtg ccgttcaccc
    22501 gtcggctgag gcccatcatc aaaaacatgt ccgagatggg cgccgcaccg ggcgcagttt
    22561 atttcagtcc ggatcatgcc ggcgctgcga tccgttttcc gggaaacgtg gtgcgggtcg
    22621 aactcgcggt agaagctcgg ccagcctgtg cccgacgtga acttgtgcgc gctaaagaac
    22681 agcggcagcc cgcacactac gcagcagtag gttccgtcct gcttgttgtc caacagcgtg
    22741 ccgcaaaacg ccggctccgt cccggacttc tgcgtgatgc ggtaggtttc cggatcgagc
    22801 ttggccgcaa gttgccgcac ctcttcgcgc gacagcggtg taatatcgta accggtagcg
    22861 gaataccgtc gttgcatttg attcacccca tcggccgccg aaaactggcc cagacagacg
    22921 atcgtcgaga gcattacagc gaccatagcg aaagtggcaa ctacggccgg ccgcgtttta
    22981 ttccgcgctc agcggcgccg aatgcgttca gggatggggg tcgggttggc cgtccgcggc
    23041 cttgacgtct gctagctgtt gcatgaccgc agttacgcgc tccaggttga tggccgcctc
    23101 ctgattgccc ggccacaatc gcaaggcctc ctggaactct tgctgggctc ttgcgaagtc
    23161 gcccagcatg gcgtgcacgt tccccagcat tttgtgggtt tcagacattt cgggattatc
    23221 ttcctgctgc acggcaagga gcgtctgaac atcgtaacgc gcccgcagct cctcttccgt
    23281 gattccggcc gccgcctggg cctcaagctc ttccgtcgag accacgctga ggccctccat
    23341 ggcacgctgc aacatgttgc gatacggctg cgtcaggtcg agctgtgcct gccgcgtctt
    23401 ctcgaacgca accagcgcct cctgcggctt gttttgcgac agcagaatta cccccaaacg
    23461 gcggtatact ttgtccggct cggcgccgca ctccagggct tcctgataga gtcttttcgc
    23521 cacctcgtaa ttatttgggt ttctccaaat catctcagcg atgttgaaca ggattacgcc
    23581 gcgcaggaac tttcccggat tctcctgcaa cgcctgctcc agaaggcgaa cgccggcgtc
    23641 cacctgaccg ctccgcgcca cggcgattgc cagatgtacg cgcgtttgcg tgtcttcgtt
    23701 gaccgccagc gcctgcttga aagcgctgat ggcccctgcg aagtcgttat tgcgaagccg
    23761 cgtgacgccg gcggccgagt accagtcggc ctcggttttt cggcccggcc gggacatttc
    23821 ggccagccag ggcgggttta tccaggtgat gatggcgaag attatgactc cgcaccacag
    23881 gatcagcagc ccgccggtga ccacggtgtg cagccacgga cgaggcaccg cgccgggcga
    23941 gctgccggcc ggtcgcagtt cgggagactc agtcattgtt gacgacgaag aggacgtttt
    24001 tgccttcctt tttcaagcgg ttaaacgttt cacaagcctg tggcgtgggc tgaattatca
    24061 cctgcgagcc gcgtttagtg ttcgggttat acataaaatg cgtttttctg ctcatgaacc
    24121 cgtggccacc ttcgccgtgc ggcccggcgc cgatcagaat tatgtccgcg cactgtttca
    24181 ggaagaatcc ccggtcgcgc ggattgaatt catgcaactt cgtcgccggg tgcagcgtgc
    24241 cgttaggcaa tatggcgaaa tcgaagtatg gccacaccag gccgcggtaa acggccaggc
    24301 cggggcgcca gtagaccatg ccgctgtcgc gatagtgcac cagggcccag gcctgtgcgc
    24361 cgatcgccag aaccgcgatg gttatcattg cccagcgcca gggtcgaaac tggctcagat
    24421 gcagtatcag gaaagtcacc acgaacgtcc cgatcgtcag gtaaatggtg atcggaattt
    24481 cgtcggcaat gttcagcagc gagcaatacg tcagcagccc ccaggcagtt acgcccaata
    24541 gcagcatcat gctgccgggc gacctgaagt actccgacag tcgccggtgt cgcagcggcc
    24601 gccaactccg cagttcactg gaaccgttgt agaccagcaa caggcccatc acgccgcccc
    24661 agacgtcctt ggatatgtcg ccggtatcga agaaacggtt gttgacgaag gtttggaata
    24721 gctcgtcgaa agtagaaagc gccagcgaga ccccgacggt caggccgatg atcgtggccg
    24781 gcttatagcc gcgcggcgtc agatcgcggt acagaattac cgcgaaaaac atgtacgcga
    24841 aatagtgcca gttcaactgc agttcataga actcgtggcc gaaatagtag tcattaatgt
    24901 actggccgta ggcgatcatc agcacggcga tacccgccgc caccagccgc ccgcggttga
    24961 gatagggcca cagcagcgcc aacagcccca ctcctacgac ggaggcgacc accagcaccc
    25021 agggcacgtc tacgcccata aacgggaatg tcgccgcgga agctcggccg atggcgtcct
    25081 gcaggtacgc gcgcagcatc aggaacggca tgcacacgac cagcagcgcc gtcgccacca
    25141 aatgcaagcg cgtcgagtga atccactcga ggcgtgaagg ggcatagcgg gtggttactg
    25201 ccgtactcat cgacgcgcac ccaaaatcgt gggccctctg gaaccgcagc gctgctcgca
    25261 ggcacgcgga tcgagttcgg cgccgaaacg tagtataccc ggccgcaatc cgtcgtccca
    25321 ggcggcgcgg gcctggcaga tccgcaaatc aacgctaaac accaccatta tcgctccaaa
    25381 ccgttcgtat tcctcaaggg cgcggcgccg ggcgcgccgc gcgggcctgc cgctccagtt
    25441 ggtccagcat ctcgatgttc ctggccagtt gccgattgtc gggccagatg cgctgcgcgt
    25501 tggtgcacgc cgcgcggcat gccgatcacc agcagcgagg tcagaatcac gggcagccgg
    25561 gtggagtgaa tccactggaa ccgggaaggg gcgtagacag cagggtgcgc tgtggtcatc
    25621 gtttcttagc gatccttcgg cgccgccgtg gccaagcact cgcaagaaga ccgccccgca
    25681 ggccggtctc ggagtagaac agtaggataa cggcagcccc caaccgtccg caacccgaat
    25741 cgcggctccc ggccgcgcga gccaaaccac gctcactcga accgcttctc gcgctccggg
    25801 cccggataac ggcgcacgcc actcgccccg ccgggtggtc ccgttgttaa gtggggagcc
    25861 cccagcgcgc atgacgggca agttccgcgt aaaaacgcgg aaaaatgagc cttccagcac
    25921 cccatctcgc gcgcgtggcc atcaatcgcg ttcacacgcg tggcgacatg gcgatcggtc
    25981 gcgtcctcgc gcacgtggcg atcgatcgcg ccctacgcta aattgccttg ttcggcgcgc
    26041 cgcttgccat aacgccggcg ctgttgctgt ttgttgatcc tggctcgtgg aggtgcccta
    26101 tgaccgacat tctgaaaaag ctcaatattt ccgaaaacta ccccggcggt ttctgcggcg
    26161 agtggctcgg ctccggccct acgctcgacg tcatcacgcc tgtcgacggc tcgcacatcg
    26221 gctccgtccg gcaagtgacg gaggccgagt acgaccgcat cgtcgcccgc gcccacgaag
    26281 ccttcctctc ctggcgcatg gttccggctc ccaagcgcgg cgaaatcgtt cgccagctcg
    26341 gcgtccgcct gcgcgaatac aaagaggaac tcggcgcgct ggtcacgctt gaaatgggca
    26401 agatcgcggc cgagggcgcc ggcgaagtgc aggaaatgat cgacatctgc gattttgcct
    26461 gcggcctttc gcgccagctt tacgggctga caatgcactc cgaacgcccg cgccatcgca
    26521 tgtacgagca gtggcacccg ctgggcgtcg tgggcgtgat cagcgccttt aacttccccg
    26581 tcgccgtctg gagctggaac gccgccatcg cggccgtctg cggcgacagc accctctgga
    26641 agcccgccag caagaccccg ctgaccgcca tcgcctgcac cagaatcgcg cagcaggtct
    26701 gcgaagagaa caacgtcgac ccggccatct tctccctggt cgtcggcccg ggccgcaccg
    26761 tgggcgagaa actcctcaac gaccggcgca ttccgctggt ttccgcgacc ggtagttgcc
    26821 agatgggcta ccgcgtcggc gaagtcgtcg gcaagcgcct cggccgcaca atcctcgaac
    26881 tcggcggcaa caacgccatt atcgttacgc cctcggccaa catggacatg gccgtccgcg
    26941 ccatcctgtt cggcgcggtc ggcacggccg gccagcgctg caccagcacg cgccgcatca
    27001 tcgtccacga gagcgtgcgc aaagacctgg taaaccggct gacgaaggct tacgcgtcgg
    27061 tgccggtcgg cgacccgcgc aagtcctcca cgctgatggg cccgctggtc acgcaccaag
    27121 cggtcgaaga catgcagaac gccatccggc gcgtcaagga agagggcggc gaaatcctct
    27181 tcggcggcga gccgctctcc ggcccggcct accccggcgg gcattacgtc aagccctgca
    27241 tcgcggccgc caagaacgaa ttccgcatcg ttcaggaaga aaccttcgcc ccgattctgt
    27301 acatcatcag ttacggatcg gccggcggat cgccggagaa agaactggaa caggccctcg
    27361 cgctgcacaa cggcgtcccg cagggtttga gttccgcgat cttcacgacc aacctgcttg
    27421 aagccgagac gttcctgtcg cacctcggca gcgactgcgg catcgccaac gtgaacatcg
    27481 gaaccagcgg cgccgaaatc ggcggcgcgt tcggcggtga aaaggaaacc ggcggcgggc
    27541 gcgaatccgg ctccgacgcc tggaaggcct acatgcgtcg ccagaccaac acgattaact
    27601 ggagcaccga gctgcccttg gcccagggca tcacgttcgg ttcgtaggca cccggaaaca
    27661 cggccggcgc cggccggcat agagaatcgc tcggccgata accaggattg gcggcatgcg
    27721 gaaacctccg cggccgccaa tttctgttgc tttcgcgttc gggatggcaa caattgaaga
    27781 ggtagcagcg ccgcgtaagc gcgcttcgcg tctgcgctgg cggtgcagcg attgagtctg
    27841 cgctggcggt gcaacggcga ccgcaggagg tgcgagaatg cttcgaagct ggttcacaaa
    27901 tggtggtagg cgctcgtcga tgtacgcctg cgtggcggcg gtcgggttgg cagcggcgct
    27961 gtgcgcgaaa gggcgcgcgg ccgaggtgat cgtcaaaaac gattcgttta cagaaggctc
    28021 accggcctac ctggtgggcg acttcgtccc cggcgaggaa gtgggcgtgc ggctcacttc
    28081 gccgtgcgac ggcacaatcg tgggcgtgca gatagcctgg ctggagggga cgccgggaca
    28141 cccgccagcc ctgcaccgcg ccattcgcat ttacgacggc agcacgtttc cgacaccggg
    28201 cgctcaattg gcgtacttgg aggcgccgct gctgtcgccg ggtttcgtca acgaattccg
    28261 gtacctggac gaagccggca cggttccgtt gtcggttccg gtaacggtgg ggcagcagtt
    28321 ctacgttgcc ctggaattcg atgagccgac ggacgttggc agcggcgggg ccagcgtagt
    28381 tcgcgacacc gacggctgtc aaaccggccg gaacatcata tatgtcattc ccggcggttg
    28441 gttcaacttc tgcctgttca tccaaggcga cgtggtcatt cgcgccgtgg tggattgcgg
    28501 cgagcagtcg ggagcgtgct gtctgccgag cggcgcgtgc cagttcatca cgcccacgca
    28561 gtgcgccgga agcgccggca cttaccacgg cgatctggtc agttgcgccg cagtggagtg
    28621 cccgcagccc agcggcgcct gctgtttcga ggcgaccggc ggctgcttgg atctgacgca
    28681 acagctttgt ggggtgacgg ccggcatatg ggcgggaatg ggaacgcagt gtgccacgta
    28741 tacctgcttc cccagcggtg cgtgctgtct gccggacggc gggtgcgtcg acgagctgag
    28801 tccgagcgaa tgtgcggccg cagccggtgt ataccagggg gatggcacgc agtgcggcct
    28861 gattgattgc cctgatccta ccggggcgtg ctgcatcgcc agcacgggcg gctgtgtggt
    28921 gctgagcgag gccggttgcg ccgtcgtggg cgcgtactgg gccggcttcg gcacaacgtg
    28981 cgtcgatctc aaccagaatg ggaaggccga cgcctgcgag gcgggcgact gcaactgcga
    29041 cggcgccgtt aacgtcttcg acatcgacgc cttcgtgctg gcgctgaccg acccggcggc
    29101 ttactacgcg acttacccga actgtttcat cagcaacgcg gatacaaacc gcgacggaac
    29161 ggtgaatgtg tttgatatcg atccgttcgt ggcgttgttg acgggcgaat gagctggctg
    29221 agggatcaag ggatctaggg atcgagaaga agagcagaga cggggcgggc agtttgccag
    29281 tttgccaagt ggttgccgga gaggcgaaca ggtgacaaag gcggctgcgc gcggtgctac
    29341 cccaggtgat cacgcggtct gataaaaagc ccgccaggat cgcgtgttgg gggcaggtga
    29401 tcagatgctc atggtttttg tgcacacgat tttgataacc tgaggcgccg ctgcgcggag
    29461 agagttgata ggtgagaggt g
//
LOCUS       DAOSYS010000011        27023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1468_length_27023_cov_5.532557, whole genome shotgun sequence.
ACCESSION   DAOSYS010000011 DAOSYS010000000
VERSION     DAOSYS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 27023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27023
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1468_length_27023_cov_5.532557"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLU99_01395"
     CDS             <1..343
                     /locus_tag="PLU99_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15737.1"
                     /translation="QVQQAVFEQQDESLRILLFRDLVNRLEKAGRPLTEAERAALNEI
                     WNERDLVQFWAVQNERAQALRIAGVDAKLASDQAIVDLLIKSIEARAKRVEQKLVAKA
                     AAEAGEAIGQQ"
     gene            425..640
                     /locus_tag="PLU99_01400"
     CDS             425..640
                     /locus_tag="PLU99_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15738.1"
                     /translation="METTVTGWRKWLWPLRSRKAQVAVATIVVAYAAHAGLELNEELV
                     TTILGVGVALILGIAHEDAGRAGTGRG"
     gene            complement(769..2316)
                     /locus_tag="PLU99_01405"
     CDS             complement(769..2316)
                     /locus_tag="PLU99_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOO15739.1"
                     /translation="MRSAFAQRPAASPEPRALIAASEDRLWTARVERDRTFLSYRDRT
                     GQPVLAEPLNARSVQLIAAGQQAYTFQEDGSFYRYSDEWAPALYLPQRQVPFDATAVD
                     GVPHALIETQAAAELPAYDAAANRPATRMFTTTAAPLTLVRYDQLGWSAVAPCPAEVA
                     AERTPELRPRLCATPGGLMLFSSTPGLDGLSCWTFEPLTGQWVSGPRLSDVAGLKGFW
                     PKLVNRLPTLVLNVADADGNGELRFFRLMGAGANYSWEPASLELSPLPPSTTGTPTPV
                     DACGFNQHLGVQVSLPDGSAYLRFGRFEGPPTLGTIDLFARPPQNVLPALLQALRAAV
                     LLAILVALFVLRRGSLVMPFPMAAGQAPAYVLQRLAAALVDLLPFTFVAALVSGLNWS
                     SAMRAAVNLISDSGAQDGPSAPVLLWMLLSAGSYAAYCLIMELLTGRTAGKMLFGLQV
                     VAEQGRRAPAVRVVVRNLFRVIEVLPFFWILAFVMLLSRNRQRVGDLFARTIVVRRVA
                     IQANSAD"
     gene            complement(2412..3230)
                     /locus_tag="PLU99_01410"
     CDS             complement(2412..3230)
                     /locus_tag="PLU99_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15740.1"
                     /translation="MFKYGGLALLPALVLLTGCPRPCRPGIAPQTAQQALDRVNNNLS
                     QIQAPVQYKGFASFRFRDAKGKDRRVLGQDASLIFAQPHSLRFDIRSLAGTIAQFGSN
                     DERFWLWVEPETNKLWWGRWASLGSGSSQLPLPADDLIDALMLRPLPTTQADGNPPDL
                     REAEGAYYLVYQRPSSTREIKLDPAPPYQPIEIIDRTPDNQIQMRAILDKYSRIDDKG
                     PYTPRKYVIYWPLDEAEMRLDATRVALRPDLPPETFAFPESWAGEVEELDAQQQ"
     gene            complement(3234..3788)
                     /locus_tag="PLU99_01415"
     CDS             complement(3234..3788)
                     /locus_tag="PLU99_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15741.1"
                     /translation="MATEWQMPQPGSACATCAHAFEPGEAFRACLYESDTGYQRRDYC
                     TRCEPPPEPAALATWQTRRPIPTKKVQIFDRAAMYEFFRGLEADPRPEKVQFRFVLAL
                     LLWRKRVIKLDRSLTVGEHEEWEFSVPATGEVHRVARPDLAEEELERLSGQLEQLLAS
                     PPGESETASTGRDPVENQPAALEE"
     assembly_gap    3812..3911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4162..6183
                     /locus_tag="PLU99_01420"
     CDS             4162..6183
                     /locus_tag="PLU99_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO15742.1"
                     /translation="MTDTAPAKTESGQISLKPPEHDLLQDGSLRPVVGGTSLPRADAV
                     QCSGLLTRPISAIQRTPVENGDARTELEKYKVSLRRYCPVQKGIAGLPWVGQWHCQRC
                     DAVVPGQIVYAAPEDAIYLEYECPTCGQYRERHHDVLFVRRLPAAPPRQPRTTFGGSP
                     IRPVVTALPKTVETLCPECACLILGRCYMRQGAVWMEKTCPEHGYFRDKITSDAELYL
                     RSMQCGFEDDRGVHRPQVTGASACPTDCGLCGQHHSTSCLAQIDLSNRCNLTCPVCFA
                     SANQAGYLAEPSYEMVVEMLQALRQQHPVPATAIQFTGGEPTLHPDFFRIVRKANKMG
                     FSHVQIATNGLKMAEPEFARRAAEAGLHTLYLQFDGLDEARYRHLRGQPLFDVKMAVV
                     ENCRATGMKICLVPTIVKGVNDDQVAEIFNFAVRNVDVISAISYQPVSFTGRISRHEL
                     ESKRYTLGDLAHDLAAASGADLQRDFFPLNHVVPLSRIMQTLDGKPKIRPTCHPQCAF
                     GTYFFVTPEREAIPIPRLFDTMRLFSGLNELAARIERERPDKLANWRDRLEIALVFLR
                     SYRWRQRDLRVTPFTFIRALRGLTSKEVGRGAGGQKTYRTLMAAGMHFMDRYNYDTER
                     IRRCVIQYSTVDGIYPFCTINAGPTYRPYLERMYSAPLTPDRLATQHSG"
     gene            complement(6304..>8049)
                     /locus_tag="PLU99_01425"
     CDS             complement(6304..>8049)
                     /locus_tag="PLU99_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO15743.1"
                     /translation="GSSPAAAAGLRPNDLILTANGEKVSSFRDIATAVILSDNGSVVF
                     QVERDGRELPDALILQTVRGAESPLVEAGISPATTTELASDVRLEDVPELRAGDRITE
                     VAGKPVSSATEFLYAFSAYVTATGASGAEITLKRPDPEDPTRSTSFKATLPAGFVTEM
                     PSTSAKSERRSEAQNVLGLSPRCKAGTVLPGQPADKAGVRADDVIVQWGTIPNPVFAE
                     IVETIQANVDRPIPIVVERDGKQIDLVVTPQRPFHLLGTPKAQVGIAFVNEDTRPIVA
                     QVADGSPASALKMPRGSQILEVAGTPTNTWFDVLHALRAAAGQTITVRYRTGNVEAEG
                     RMTVPSSVVDALNLPPLVLVTSIAGESRLTQGDEVLRLPAVSAIRGLLEKHKGETVTV
                     EYQTAPDDPQIHRGEFTVAADGSNTDPWQMRVLLLPKPSNIPFKHAQTVVRVNNPLTA
                     LGLGTRQTGAVLVEMYRLVTAIVKNLAQGDTSTVQHVSGPVGIVGFAVERAKLGYVEL
                     LSFLAFLSVNLAVINFIPFPVVDGGLMVFLIIEKIKGKPLSLKTQMVATLVGLATIIL
                     VFVLVTFKDITNMLS"
     assembly_gap    8050..8149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8150..8382)
                     /locus_tag="PLU99_01430"
     CDS             complement(<8150..8382)
                     /locus_tag="PLU99_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO15744.1"
                     /translation="MNTLAAGDLDWLGGTLGLIKVLIGFSIIIFVHELGHFLAAKWAG
                     IRVDRFAVGFGYRLFGWRRGEGFTFGKRPNYTT"
     gene            complement(8382..9539)
                     /locus_tag="PLU99_01435"
     CDS             complement(8382..9539)
                     /locus_tag="PLU99_01435"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOO15745.1"
                     /translation="MHHRLIILGSTGSIGCSALDVVRGLGPDVRVVGLAAGRDWAKLA
                     EQARRFRVGTVAIADETCAADLARACPPNTRVLAGPQGLVELVQTTDADFVLAAIVGV
                     AGLPATLAAVERGIDVGLANKESLVVAGSIIMPLARQRGCRLIPVDSEHSAIFQALMS
                     GRPEEVRRIYLTASGGPFRTWPKERIAQATIAEALDHPTWSMGPKITIDSATMMNKAL
                     EIIEAHYLFDVPVDRLQVLVHPESIVHSMVEFRDGSVIAQLGTPDMRTPIQYAITYPQ
                     RAPGLAQPLTWDTARRLNFEPPDPQRFPALTLGHEAAQRGGTCGAVLNAANEAAVERF
                     RAGEIQFGQIAELAGRTLRRHRGVAQPALADLMAADAWARQEVDECIRVGA"
     gene            complement(9736..10869)
                     /locus_tag="PLU99_01440"
     CDS             complement(9736..10869)
                     /locus_tag="PLU99_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO15746.1"
                     /translation="MAVFLVGGNAEAWCPFGGVEALYTYAREGNLICSLGVSNFYILG
                     GVLLSVLLLRRAFCGYVCPIGAISEWLQAAARRLRIRPRRVPYGLDRGLALLKYAVLG
                     LILYFTWTAGELVFRGYDPCYALLSRHGEDITFWAYVISGAILLISLFVKVPFCRWFC
                     PLAAVFTPFSRFGLMRIKRSPDACTDCGACSRVCPMGIRVHTQANVTAARCTACLECV
                     AVCPTRKRGALTWGLPRHLDRRGRLAVTSGPWPQAALIAILLLCIGAAVAATYTFPLP
                     SFVQTRGTAPPTTATLQLNIEGVTCRGSANLLAYFLERDDDLEIPGYLKIEAWPGPGA
                     SAVHITYDPSQTTPDTIRNAIKQPYFDLVINQFRVSPFQITEP"
     gene            complement(11096..11995)
                     /locus_tag="PLU99_01445"
     CDS             complement(11096..11995)
                     /locus_tag="PLU99_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15747.1"
                     /translation="MKLKGVLVGLLCVILPAASWAGDGDGQAQFTQGTELLSKGDLDG
                     AAKAFEAAAKAEPDNQNYQVRAAIVQRVLAMRQTLDEQTDNEKWLNTAKALHTFYDEN
                     GIHDQALALANRAHTKLNTTETAVMLARSRLALGMNSEAAEGLRGITQSKIDAQVRVL
                     LGLALARQGQKDEAAKLLGTLKDDAPESPQCEFDLARLHTLLGQHKEATAALVRCFET
                     TPPSGLDAIKEQARTCDDLKPLQSKPEFKKALDTQSKVAESKCSKGPDCGKCPLKKKC
                     QGKDQKSHEDCKGHGKSNAPSKP"
     gene            complement(12075..12530)
                     /locus_tag="PLU99_01450"
     CDS             complement(12075..12530)
                     /locus_tag="PLU99_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15748.1"
                     /translation="MRQSLVIWLLTGALLGSAALNAFLVGQRRTSASPCAAGGFCPIT
                     LDRPNCPLADELQLSEEQRVKIFSCCGGGVCLRQREQQAKVLEDSLAQLEQALTADKI
                     DSQLVESLADKIAGLRAQEWKDRIHCVLQVRMALTPEQLARLNTERKAP"
     gene            complement(12527..13018)
                     /locus_tag="PLU99_01455"
     CDS             complement(12527..13018)
                     /locus_tag="PLU99_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOO15749.1"
                     /translation="MTCQDAQKQLSAYHDGELDPARREDLVAHLQTCPACRAYLARLS
                     LLNRVLQSGTPAEVPANLLPRVLGALASQRRRRRIREWGIRITAAAAGLALCVTAQRL
                     LIVRPARPDAASAEASIAVELALRELQGAVAGAGLQDRELDALARRPEVVLFEELTGR
                     LRP"
     gene            complement(13126..13692)
                     /locus_tag="PLU99_01460"
     CDS             complement(13126..13692)
                     /locus_tag="PLU99_01460"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOO15750.1"
                     /translation="MDPDARREPDAETVGRLRSGDIAALEAVFWALSDAVLRLSRALM
                     GNSADAEDAAQEIFLRVYEQAGKFDGQARFSTWVYRLAVRYCLNKLRQRRRRAAAEKA
                     AVAQRIRLVTAEPGRLPATKDENARAAALLERLPPSYRACLVLREIEGRSYAEIAELL
                     DIPPGTVMSRLSRARRLLAAMLDETTEK"
     gene            13797..14717
                     /locus_tag="PLU99_01465"
     CDS             13797..14717
                     /locus_tag="PLU99_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO15751.1"
                     /translation="MSQDQAACAPPVIVARKLTRKFGNLEAVAGVDFQVQPNQCIGFL
                     GPNGAGKTTLMRMIYSAIEKTSGELTVFGLTPMVDRKLINSRLGVVFQDNNLDEDLDA
                     AECLHVHALYCGLDWPTARRRTAELLEWLSLADKARSRIRALSGGMVRRLMVGRALLN
                     GPELLILDEPTTGLDPQVRHVIWAMLRELRRQGITILLTTHYMDEAQQLSDSIMLMDR
                     GRIILSGRPQELIERHLERYVLQVSGLESRPALSADVRHEHVGDTDYFYSHTEPPLQA
                     LYRQLPQNALLRHANLEDVFLKFTGRGLHE"
     gene            14736..15650
                     /locus_tag="PLU99_01470"
     CDS             14736..15650
                     /locus_tag="PLU99_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15752.1"
                     /translation="MTERERGRHEELIEQELARAAQAAQPAAEPVLGVRPPQLGYFRT
                     QTRLRTGWFWSLYGVWYRHVRVYTKTLLANAAPPVFEPLFFFTAIALGLGSYMPNASF
                     DGLPYATFVASGLVVASAMFTSVFETTFGTFVRLVYQKTYDAMLGTHLALNQIFIGEL
                     LFTATKGAVFSSIVLGVTAVFGVRPTAWCVLVPIVGAATAYLFGAIGLIVTSYVRMIN
                     NFSFFTSGIITPLFFFSGTFFPVRGHNAVLTVFASLLPLTPAIELSRALYKGEFTRVT
                     LAYLAVFCVYIVICHVVSLRRMRKRLLS"
     gene            complement(15765..17114)
                     /locus_tag="PLU99_01475"
     CDS             complement(15765..17114)
                     /locus_tag="PLU99_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15753.1"
                     /translation="MTRTLHIVAAMTASLLIATVAQAQPGLAEVGPIDPVKGFPQWIQ
                     DWNGVALQIGLDGDGSTGLSLFDPVDPEFPFSAVVGFGAEAFYMSAESSITLPGNRDA
                     RLVLAVEAAFAAEEPIDGDQTVFARVRIRIDTPVAGDYTVIHPYGVNFFPNRPAGDRG
                     INYTSDIGGITPDFSRVLGGAIGPYLRAVNPPPPDGYIGNPQVDQTVTGSPFGTNFFR
                     IEGPPGADLDGAGNNFVQTELFQLCGKHFDGQTPTPMRLDRATYSRNTLGKFIDVYAT
                     AVPTATLIVNAGPTIVDVPMITDGTGNYFARLPVGAETPLPATVAVTGTNPPSSLPTT
                     LTTNLVDAVHVTRALYDATNHVLTIDATSSDTLPRPTLTVVGYGTMSPSWRLVVPDVA
                     VPPGSVTVTSSAGGSGTRQMDATGASTLPPLPLSARATVGGAAVSPSSVYPVEPVSP"
     gene            complement(17146..18522)
                     /locus_tag="PLU99_01480"
     CDS             complement(17146..18522)
                     /locus_tag="PLU99_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOO15754.1"
                     /translation="MPAFHIPRQSVIGMEGHWAKMFDPPNESPDTPEFVEPNNPVPEV
                     MLAAGYLDRVTTPHTVQRDLMHGDDIDTWDGLKELVFFLFRDRDNPADDGHFPGATIR
                     VPRGVIFHCETQGHGPPPHTIHWHGIEPTPMNDGVGHCSMEIGHYIYQWQPNFIGSYF
                     YHCHRNTVQHFEYGLYGLLLIEPPDAYFASIASIDPDGTYHLNDIPIGACSDGKFRVA
                     ANLARFPQFPGFIGGDPVEGVANGDPHAFTVPYDVEVLWVPDDRDSVWSDLAPDARAT
                     YPRFGSTPGVDDEFDRNPGHDDFFAFNDFNADYWFITGVPVPAHKGGTAAIPPGLVIP
                     PELNSGVSGSQISVNARVGQTILVRCIDAAYNSISVTLPVDAVIIAMDGRALGVPPFG
                     LYNSAFILPAGVPFHLSTARRFDALIRPTQPIDSFATVQFIDTRGQVPGTPEVVLMTA
                     RIPFVVTP"
     gene            complement(18560..19990)
                     /locus_tag="PLU99_01485"
     CDS             complement(18560..19990)
                     /locus_tag="PLU99_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOO15755.1"
                     /translation="MALSRREFLARGLGGSAALVVGSAAQKMPFLPHYASLSVQTLNF
                     HISDAIKDMVTHNAINEAQCYFWVFKEERWPAEVPGPIILTTYRETIEITITNDLDEP
                     HAFYIPGMFNSGPIQPGETYKGSFQASKAGTFLYYDNLNEPVNRMMGLHGAFIVMPAA
                     PQPGHKYTPYVVPTAAVQRLFDDFGSAPWWPGLAWEEGDHDTHTLAFRQYVWLLHQAS
                     PNLFAEVGELPPGEIYPGDQFMEAFLRDPFSPTGDNRIPQYFTINGQSGHFAHNSPHL
                     CPNNRVGEPVIIRVLNAGLWTHSIHIHGNHVYLLALNGAVQKNALWVDTFTARPMDTF
                     DWAVPFMRPPDIPNERGIGRADPGLPTTGGGTTWPPNEELALFIPAIGDRMGLDLAGE
                     PIDLSVQLSPLCYPMHDHSEPTQTSQGGNYNCGLVSGIDFTGDRNTPSGVTTFPIGSE
                     HEGHGPEATGPAAPPIGEYPQGTAKW"
     gene            complement(20049..20303)
                     /locus_tag="PLU99_01490"
     CDS             complement(20049..20303)
                     /locus_tag="PLU99_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15756.1"
                     /translation="MSFLLSLTDERVRQEQAPFDHPQLFVPIGQFGDESAIVGSSRVL
                     RDGFRENEEVLEIPAVGVGGRVEALLPPLGTFLNLSPFQP"
     gene            complement(20300..22177)
                     /locus_tag="PLU99_01495"
     CDS             complement(20300..22177)
                     /locus_tag="PLU99_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOO15757.1"
                     /translation="MRRRAKYSASGVDYRTCCTVELLLLITVVVGPGALPAQAQPPAP
                     AEPALPPPSLKTVIVPEPPNLHLYVRNRQAAIQLGKALFWDMQVGSDGVTACASCHFH
                     AGADNRIKNALSPGIRAGDETFQVAGPNYTLHFTDFPLRALSDPEDRDSPPLWESNDI
                     VSSLGQFQTDFIQTRLGFPLDLGTEVADPIFHVGAVNTRQVLFRNTPTVINAVFNAAN
                     FWDGRASHFFNGVSFSGPLDPNSGVFVNIEGVLGKQPLLISQASLASQAVAPPLASTE
                     MSFAGRSWPQIGRKMLNLRPLDKQMVHPRDSVLGPLSRARLARPLDSKVSASDDTVPE
                     TSGSPVSLGGVPLAGQRGLFTTYEALIRDAFQPQYWNSDLIIEYQPDGGSSVIPRPAG
                     MLAGNQFTQMEANFSFFFGLAIQTYESTLVADDTPFDRYLDGDPDALTAEQLAGFDIF
                     LDRGRCAQCHTGPELTEAALRGIELMFMASPGRAFYDRGFINIGVRPTAEDIGQGGTA
                     PFINPVTGQPYPLSLTRLAMLKRAGQLPPAVSLFVPDLPFGTGSPDPNRVSVDGSLKV
                     PGLRNVELTGPYFHNGGQATLRQVVDFMTAAATSSTRTSTTCMRSSANWNSARMTRTP
                     W"
     gene            complement(22211..22486)
                     /locus_tag="PLU99_01500"
     CDS             complement(22211..22486)
                     /locus_tag="PLU99_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15758.1"
                     /translation="MSDLLSSHRQAAFYPELLDALSDLSPTTLSDFTGPLAGDLTFAF
                     QWDLTLGVGEVASLTLNENLIPEPASLVCLALCAAILLQRRLRGCVP"
     gene            complement(22483..23007)
                     /locus_tag="PLU99_01505"
     CDS             complement(22483..23007)
                     /locus_tag="PLU99_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15759.1"
                     /translation="MTQRILLIAVVTCYCGGVTGPAVAELVTLSSGNSVARIDTGSAL
                     GLYSWTVDGVEHLSAQQLWFRIGDNEENPISSVEPVGFTTDGQSATVQFAQGLFDVDL
                     NYALLGGALGSGQSQLAHIITLTNVADVPLDLHLFQYVDLDVDGEFDFNSLSFLTNDL
                     ARQIGPSGSIATVA"
     gene            complement(23444..24763)
                     /gene="serS"
                     /locus_tag="PLU99_01510"
     CDS             complement(23444..24763)
                     /gene="serS"
                     /locus_tag="PLU99_01510"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOO15760.1"
                     /translation="MIDLKLIRENPDLVKKAVRDKNMRVDIDQIIAIDARRRALETEF
                     NTLRHQQNTTGERIAKAPKEEKAALSNEMREIKARLKEIDAEREKVDAELNELMLLVP
                     QIPHPAAPIGPDESGNVEVRRVGAPKAKEAFEPKFRDHVELGEQLGLFDIDRGVKIAG
                     SRNYVLTGLGAMLHEAVLRLAWDVMLAGRFDKPGAPDVKLQFKPLTVPVLVNDSLMYG
                     TGFFPLHREEVYLCERDGQSLVGTAEVPVTGLHSDEILDQAELPKLYFARSTCFRREA
                     GAGGKDTRGLYRIHYFDKVEQVVVCQADADESAYWHDVIISNSERVLQALELPYRVVE
                     VCTGDMGQGKVRMYDIETWMPSRDGYAETHSASRFHDFQARRLNIRYRRADTGKPEFC
                     HTLNNTVIASPRILIPIMEMYQTPQGGVRIPERLRPYLNGVEVIGPG"
     gene            25101..26639
                     /locus_tag="PLU99_01515"
     CDS             25101..26639
                     /locus_tag="PLU99_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO15761.1"
                     /translation="MAGLKAAPGDAHSIAANYVNSAVGVGLLPQGCQSATAANPRSFQ
                     HALCVYPYRRELNEAGFFPPLGLELIAAVVQQYARSLDLIDLRMVANRTRSYLRPQTD
                     LVCFSINWDRDREFLEEEIRSVPPGILTVIGGRHATENPGRWLADFPNVDLVIRGDGE
                     DALDEVCRGVPLNQIAGLSFRQNGLIVHNPNRSLGHVRDDLYPDREARRQDYVVTIKG
                     ARAGIAVDSIATSRGCPNHCAFCSFNRNPWGEMRRWSARSPESVVDELGRIQARLIGF
                     VDDLFTHDLERVERICDLILARGIRKRYFVNARLEIARRPDVLRKMEQAGFSMLMLGV
                     ESAHDKTLRSMRKGFDVARIRKYFEVLRHSRMFLHGYFIVGCVGESREDMLRIAPFAR
                     ELGLDTIALSVLRSSPYSGLDELVASSPGYHIAPNGKIYSDQCSVKELGALRRRIYHQ
                     FYTPGQIARVTLKALRNDALSFLPGFLPHLPQVSVAALSHAWRKARDRRRKRRLRSVA
                     QPAE"
     gene            complement(26563..>27023)
                     /locus_tag="PLU99_01520"
     CDS             complement(26563..>27023)
                     /locus_tag="PLU99_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15762.1"
                     /translation="QLSGQAGLLAFGPSEYAPQLIELQAELLRRPQTSPQACARAAQL
                     RQKYAESLRLAIEAGEVAAYVPSGFISAAHSAPEYPGSADEVREALASLHNIEQVEIV
                     AIGDFSREDLLGAVEAAWPAAGGTSATQQAGQRCGGAASAGGHELCARRG"
BASE COUNT         4937 a         8304 c         8514 g         5068 t
ORIGIN      
        1 ccaggtgcaa caggccgttt ttgaacaaca agatgaatcc ttgcgaatcc tgttattccg
       61 cgacctggtg aaccggctgg agaaggcggg gcggccgctg acagaggccg agcgggctgc
      121 gttgaacgaa atttggaacg agcgcgacct ggtgcagttc tgggcggtac agaacgagcg
      181 cgcccaggcg ctgcgcatcg ccggcgtcga tgccaagctg gcgagcgatc aggccatcgt
      241 cgacctgctg atcaagtcca tagaagcgcg ggccaaacgc gttgagcaaa aacttgtggc
      301 gaaagccgcg gccgaggcgg gagaggcgat tggtcagcag taaacagagg taattgacgg
      361 gcttgaagaa gaaactgcca ttcaatggcg ccggagttga gagccaagaa gaaaggagta
      421 gcaaatggag actaccgtta cgggctggcg caaatggctt tggccgctgc gcagccggaa
      481 ggcacaagtc gccgtggcaa ccatcgtggt agcttacgcg gctcacgccg gcctggagct
      541 gaacgaggaa ctggtcacga cgatcctggg cgtgggcgtc gccctcattc tgggcatcgc
      601 tcacgaggac gcgggccggg cgggaaccgg gcgaggatga caactgccgc gttgcgaacg
      661 ccagaagcgg tgcgggcaaa tgcgcggatg ctgaggttct tggcggcatc cggggcgaca
      721 cgccccggct ctgctacggg gggcacgccc cagctctgct gtgcggtttt agtctgcgga
      781 attcgcctgt atcgccactc gccggacaac gatcgtgcgg gcgaacaggt cgcctacccg
      841 ctgccggttg cgcgacagaa gcatcacaaa ggctagaatc cagaagaacg gcagcacttc
      901 gattacgcgg aagagattgc gcacgacaac gcgaacggcc ggggcgcggc ggccttgttc
      961 ggcgacgact tgcagcccga acaacatctt gccggccgtc cgccccgtga gcagttccat
     1021 aattagacag taagccgcat aactgccggc actgagcagc atccacaaca acacaggcgc
     1081 cgagggaccg tcctgcgctc cggagtcaga tatcaggttt acagccgcgc gcatagcgct
     1141 cgaccaattc aaacccgaca ccagagccgc gacaaaggtg aatggcaaca ggtcgaccaa
     1201 cgccgccgcc aaccgctgca acacgtaggc cggcgcctgg cccgccgcca tcgggaacgg
     1261 catcaccaga ctgccgcggc gcaggacgaa cagcgccacc agaatggcga gcagcacagc
     1321 cgcacgcagc gcttgtaaca gcgccggcag cacgttctga ggcggcctcg caaacaagtc
     1381 gatcgtgcct agcgtgggcg ggccctcgaa gcgtccaaac cgcaggtatg cggacccgtc
     1441 cggcagactg acctgcacgc caaggtgctg gttgaagcca caggcgtcga ccggcgtcgg
     1501 cgtcccggtc gtactgggcg gcagcggcga gagctcgaga ctcgccggct cccaggagta
     1561 attggctcct gcgcccatga gccggaagaa ccgcaattcg ccgttgccgt ccgcgtcggc
     1621 cacgttcagt acgagcgtgg gcaagcgatt gaccagcttg ggccagaatc ctttcaagcc
     1681 cgccacgtcc gaaaggcgcg ggccggatac ccactggccg gtcaatggct cgaaggtcca
     1741 gcaactcagg ccatccaaac caggcgtgga tgagaagagc atcagaccgc cgggcgttgc
     1801 acagagccgc ggacggagct ccggtgtgcg ctcggcggcg acttcggccg ggcacggcgc
     1861 caccgccgac cagccgagtt ggtcgtagcg gaccagagtc agcggcgccg cggtggtcgt
     1921 aaacatgcgt gtggccggcc gattggcggc agcgtcgtag gccggcagct cagccgcagc
     1981 ttgtgtctcg attaatgcgt gcggaacgcc gtccacggcg gtcgcatcga aaggaacttg
     2041 ccgctgcggc aggtaaagcg ccggggccca ctcgtcagag tagcggtaaa acgagccgtc
     2101 ttcctgaaac gtgtacgcct gctggccggc ggcgatgagc tggacgctac gtgcattgag
     2161 cggctcggcc aaaaccggct ggccggtgcg gtcgcgatag gacaggaacg tgcgatctct
     2221 ttcaacgcgt gccgtccaga gccggtcctc actggcggcg atcagggccc gcggctccgg
     2281 cgacgctgcg gggcgctgag cgaacgccga gcgcacggtt aacacgcaga gaaggaacgc
     2341 cagcagatgc catgcgggcg cggatttcgg acgaaaaagg cgtccagatc gcgaattccg
     2401 aatgagcaac ctcactgctg ctgtgcgtcc aactcctcga cttcgcctgc ccagctttcc
     2461 ggaaacgcaa atgtctccgg cggcagatcg ggccgtaacg ccactcgagt ggcgtcgagc
     2521 cgcatctcgg cctcgtccag cggccagtat atgacgtact tacgcggcgt atacggtccc
     2581 ttgtcatcga tccggctata tttgtcgaga atggcccgca tctgaatctg gttgtcgggc
     2641 gtgcggtcga taatctcgat cggctggtac ggcggcgcgg ggtccagctt gatttcgcgc
     2701 gtgctggacg gccgctggta taccaggtag tacgcgccct ccgcctcgcg caggtcgggc
     2761 ggattcccgt cagcctgcgt ggttggcagt ggccgcagca tcagcgcgtc aatcaaatca
     2821 tcagccggca acggcagttg actcgaaccc gaccccagac tcgcccagcg gccccaccag
     2881 agcttgttcg tttcaggctc aacccacagc cagaaacgct catcattcga gccgaattgt
     2941 gcgattgtgc cggcgagtga gcggatatcg aagcgcaggc tgtgcggctg agcgaaaatc
     3001 agagacgcgt cctggcccag gacccggcga tccttgcctt tggcatcgcg gaagcgaaaa
     3061 gacgcgaagc ctttgtactg caccggggct tgaatttgag acagattgtt gttgacgcga
     3121 tcaagggcct gttgcgcggt ctgcggcgcg attcccggcc tgcatggccg cgggcaaccc
     3181 gtcagcagaa ccagcgctgg cagcagtgcg aggccgccat acttgaacat gcttcattcc
     3241 tccagcgcgg caggttgatt ctcgacaggg tcgcgccctg tcgaggcggt ttcggactca
     3301 cccggcggcg acgccaggag ttgttcgagt tgcccactga gccgttccag ctcctcttcg
     3361 gccaggtcgg gccgcgccac ccgatgcacc tcgccggttg ccggaacgct gaattcccac
     3421 tcttcgtgct cgccgaccgt caggctgcgg tcgagtttaa taacgcgctt gcgccacaac
     3481 agcagcgcca ggacaaaacg gaactggact ttttcgggtc gtgggtcggc ctccagcccg
     3541 cggaagaatt cgtacatcgc ggcgcgatcg aagatctgga ctttcttcgt gggaatcggg
     3601 cggcgagttt gccacgtggc caaagcggcc ggctcgggcg gcggttcgca cctggtgcaa
     3661 tagtcgcggc gctgatagcc cgtgtccgat tcgtacaggc aggcgcgaaa tgcttccccc
     3721 ggctcgaacg cgtgagcaca ggtcgcacaa gcgctgcccg gttggggcat ttgccattcg
     3781 gtcgccaatc acaccctcct accgaggata annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn ncatttcggg gcgacacgcc ccagctctgc aaggcgtcgc gggcccgtgc
     3961 gaccatggta agatagtaat tagtaatggt ggctgggccg acccgcggtg cgggcggctc
     4021 cggttcggcg cgggctgagc atgcccgccg gcgcgctgcg tggcgcgtct cgattgatac
     4081 aggctgcgcg aatttgccaa cccggcacga tggaccgcgc attcggagcg ttcgcatggc
     4141 agagtcggga tatccttgcg catgacagac acggcaccag ctaagacgga atccgggcag
     4201 atttcgctca agccgccgga gcacgacctg cttcaagacg gcagtctgcg accagtcgtt
     4261 ggcggtacaa gcttgccacg ggcggacgcg gtgcaatgct ccggtctact gactcgtccg
     4321 atcagcgcta tccaacgcac gcctgttgag aacggcgacg cgcgcaccga actcgaaaag
     4381 tacaaggttt cgctccgccg ctactgcccc gttcagaagg gcatcgcggg tctgccctgg
     4441 gttgggcaat ggcattgcca gcgctgtgac gccgtcgtgc ccggccagat cgtctacgcc
     4501 gctccagaag acgccattta ccttgaatac gaatgcccca cctgcggcca gtaccgcgag
     4561 cggcaccatg atgtgctctt cgttcgccgt ctgccggccg ccccgccgcg ccagccgcgc
     4621 acgacgttcg ggggttcccc gatccggccg gtcgtgacgg cgttgcccaa gaccgttgag
     4681 actctctgtc cggaatgcgc ctgtttaatt ctcggacgct gctacatgcg gcaaggtgcg
     4741 gtctggatgg agaaaacctg ccccgaacac ggctacttcc gtgacaaaat aacttctgac
     4801 gccgagctgt acctgcggag catgcaatgc ggattcgaag acgaccgcgg cgtgcatcgc
     4861 ccgcaagtca ccggcgcgtc agcctgtccg accgattgcg gcctctgcgg gcagcaccac
     4921 agcacctcct gcctggccca gatcgacctc agcaatcgtt gcaatctcac ttgcccggtc
     4981 tgcttcgcca gcgccaacca ggccgggtat ctcgccgagc cttcctacga aatggtggtc
     5041 gagatgctgc aggccctgcg tcagcagcat cccgtacccg cgaccgccat ccagttcacc
     5101 ggcggggagc ccacgctaca cccggatttc ttccgcatcg tgcgcaaagc caacaaaatg
     5161 ggcttttcgc atgtccagat cgcgacgaac ggcctcaaaa tggccgaacc tgagttcgcg
     5221 cggcgcgccg ccgaggccgg cctgcacacg ctttatctcc agttcgacgg gctggacgag
     5281 gcgcgatatc gccaccttcg cggccaaccg ctgttcgacg tcaaaatggc ggtcgtcgag
     5341 aactgtcgcg ccacggggat gaagatttgc ctcgtgccga ccatcgtcaa gggcgtcaac
     5401 gacgatcagg tggccgagat cttcaacttc gccgtccgca atgtggacgt gatcagcgcc
     5461 atctcgtatc agccggtctc cttcactggc cgtatcagcc gccacgaact ggagagcaag
     5521 cgttacacgc tgggcgacct ggcgcacgat ctggcggccg cgagcggcgc ggatttgcaa
     5581 cgcgatttct tccctctgaa tcatgtcgtg ccgctctcgc gcatcatgca gacgttggat
     5641 ggcaagccga agatccgccc cacgtgccat ccgcagtgcg cgtttggcac gtacttcttc
     5701 gtgactccag agcgcgaggc catcccgatc ccgcgcttgt tcgatacgat gcggctcttc
     5761 agcggcttaa atgagcttgc ggcgcgcatc gagcgcgagc ggccggacaa gctcgccaac
     5821 tggcgcgacc gccttgagat cgcgctggtt ttcctgcgca gctatcgctg gcgacagcgc
     5881 gacctccgtg ttacgccctt cactttcatc cgcgcgttgc ggggtctcac aagtaaagag
     5941 gtcggccgcg gcgcaggcgg gcagaagacc tatcgcacgc tgatggcggc gggcatgcac
     6001 ttcatggacc gctacaacta cgacacggag cgcatccgcc gctgcgtcat ccaatacagc
     6061 acggttgacg gcatctaccc cttctgcacc atcaacgccg gcccgacgta tcggccgtat
     6121 ttggaaagaa tgtattcggc cccgctcacg ccggacaggc ttgcaactca gcacagcggc
     6181 tgatgcttcg gcggaccgta gttcccggag gacattgcgc gaatggggct aatatgcccc
     6241 agcgctgctc gcggcgtacc tcttgatccc ccgatccctt gatccctttg ctctggcgtg
     6301 gggctagctc agcatgttgg tgatgtcctt gaacgtgacc agcacgaaca cgaggatgat
     6361 cgtggccagg cccaccagcg ttgcaaccat ctgagtcttc aaactcaacg gcttgccttt
     6421 gatcttctcg atgatcagaa agaccatcaa gccgccgtcg actaccggga atggtatgaa
     6481 gttgatcacc gccaggttca ccgacagaaa cgccaggaaa ctcagcagtt ccacgtaccc
     6541 gagcttggcc cgctcgaccg caaagcccac gattccaacc gggccggaga cgtgctgcac
     6601 cgtgctcgtg tctccctgcg cgaggttctt gacgatggcg gtcaccagac ggtacatctc
     6661 gaccagcacc gcgccggttt gccgcgtgcc cagtcccaag gcggtcaacg ggttgttcac
     6721 ccgcacgaca gtttgcgcgt gtttgaacgg aatattggac ggcttcggta gcagcaagac
     6781 gcgcatctgc catggatccg tgttggaacc gtcggccgcc actgtgaact cgccgcgatg
     6841 aatctgcggg tcgtcggggg cggtctggta ttccaccgtt accgtctccc ccttgtgttt
     6901 ttccaacagt cctcgaatgg ccgacacggc gggcagacgc aacacttcgt cgccctgcgt
     6961 gagccggctc tcgccggcga ttgatgtcac caacaccagc ggcggcaggt tcagggcatc
     7021 gaccacgctc gagggcactg tcatccgtcc ctcagcttcg acattgcccg tgcggtagcg
     7081 caccgtaatc gtctgtcccg ccgctgcacg cagagcgtgc agtacgtcaa accaggtgtt
     7141 ggtcggcgtg ccggctacct ccaggatttg tgagccgcgc ggcattttca aagcgctcgc
     7201 tggactgccg tcggccactt gggcgacgat aggccgggta tcctcgttca caaatgcgat
     7261 gccgacctgc gccttgggcg tgcccagcag gtgaaacggc cgctggggcg taacgaccag
     7321 gtcgatctgt tttccgtcgc gctcgaccac gatcggaatc ggccgatcga cgttggcctg
     7381 aatggtttcc acgatttccg cgaacacggg attgggaatc gtgccccact gcacgatcac
     7441 gtcgtcggcg cgtaccccgg ccttgtcagc cggttgtccg ggcaacacgg tgccagcctt
     7501 gcagcgtggg ctgaggccga gaacgttctg cgcctcactg cggcgctcag atttcgcgga
     7561 ggtagagggc atctcggtca cgaaacccgc cggcagagtg gctttgaaac tcgtgctgcg
     7621 cgtgggatcc tctgggtcgg ggcgcttaag ggtgatttct gctcctgacg cgccggtagc
     7681 ggtcacgtac gcgctgaacg cgtacaggaa ctccgtcgcg ctactgaccg gcttgcccgc
     7741 aacttcggtg atccggtcgc ccgcgcgcag ctcagggacg tcttccaggc ggacgtcact
     7801 cgccaactcg gtcgtcgtgg ccggggaaat accggcttcc accagagggc tttcggcgcc
     7861 gcgcaccgtt tgcagaatta gtgcatccgg gagttcgcgg ccgtcacgct ccacctggaa
     7921 aaccacggag ccgttgtcgg acaggatcac cgccgttgcg atgtcgcgga agctggagac
     7981 tttttcgcca ttggcagtca ggatgagatc gtttggtcgc aggccggcgg cggccgcggg
     8041 gctggagccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ccgtcgtgta
     8161 gtttggccgc ttaccgaatg taaacccttc gccgcggcgc cagccgaaga ggcggtaacc
     8221 gaagccgacc gcgaagcggt cgacgcggat gccggcccat ttagcggcca gaaaatggcc
     8281 caactcgtgg acgaagatga tgatcgagaa accgatcaac accttgatga gtccgagagt
     8341 gccgccgagc cagtccagat cgcctgctgc gagtgtgttc atcaggctcc aacccgaatg
     8401 cattcatcaa cctcttgtcg cgcccaggcg tcggcggcca tcaggtcggc cagcgccggc
     8461 tgcgctacgc cgcggtgccg ccgcaaagtc cggccggcga gttccgcgat ctgtccaaat
     8521 tgaatttcgc cggcgcggaa gcgctcgacg gcggcctcgt tggccgcgtt cagcacggcc
     8581 ccgcacgttc cgccgcgctg tgcggcttcg tggcccagag tgagtgcagg gaagcgctgc
     8641 ggatcaggcg gctcgaaatt cagtcgccgc gctgtgtccc acgtcagggg ctgcgccaag
     8701 cctggggcgc gctgcgggta cgttatagcg tattgtatcg gggttcgcat gtcgggggta
     8761 ccgagctgag cgatgacgga accgtcccgg aattcgacca tggagtgcac gatggattcc
     8821 ggatgcacca ggacttgaag ccggtctacc ggcacgtcga acaggtagtg cgcttcgatg
     8881 atttcgagcg ctttgttcat catggtggcg ctgtcgatcg tgattttcgg gcccatcgac
     8941 caggtggggt ggtcgagggc ctccgcgatc gtggcctggg cgatccgctc tttcggccag
     9001 gtgcggaatg ggccgccgga ggcagtgagg tagattcgcc gcacttcttc ggggcggccg
     9061 ctcatcaggg cctggaagat ggcggagtgc tcgctgtcga ccgggatcag gcggcagccg
     9121 cgctggcgcg cgagcggcat gatgatagag ccggcgacga cgagggattc cttgttcgcc
     9181 aggccgacgt cgatgccgcg ctcgaccgcg gccagcgtgg ccggcaggcc ggcgacgccg
     9241 acgatggcgg ccaggacgaa atcggcgtcg gtcgtctgga ccagctcgac cagcccctgc
     9301 ggtccggcca ggacgcgcgt gttcggggga caggcgcggg cgaggtcggc cgcgcaggtt
     9361 tcgtcggcga tggcgaccgt gccaacccgg aagcggcggg cctgctcggc gagcttggcc
     9421 caatcccgcc cggcggccag gccgacaacg cgcacgtcgg gtcccaaccc gcgaactacg
     9481 tcaagggcgg agcaaccgat cgagccggtc gatccgagga ttatcagccg gtggtgcatt
     9541 cggggtagta tagcgggggg ttggcggacg acgccatgcg ggcgtgataa ggtgatagcg
     9601 tgataaagtg ataacggcgc ggccgggcta cgtcgactgc gcgaatcctt atcaccctat
     9661 cactttatca ctttatcacg gcaacatggt tgccatggat gctaacctta taaggtgaac
     9721 ggcgccgcgt cggcgtcagg gttccgtaat ttggaagggg gatacgcgaa actggttgat
     9781 taccaggtcg aagtagggtt gtttgatggc gttgcgaatg gtgtccggcg tggtctgcga
     9841 cgggtcgtac gtgatgtgaa ccgcggatgc gccggggccg ggccaagctt cgattttcaa
     9901 ataacccggg atttccaggt cgtcgtcgcg ttcgagaaag taggccagca ggttggcgct
     9961 gccgcgacag gtcacgcctt cgatgttcaa ctgcagtgtg gctgtcgtgg gcggcgccgt
    10021 gccgcgcgtc tggacaaacg agggcagcgg aaacgtgtac gtcgccgcta cggcggcgcc
    10081 gatgcagagc aacaagatgg cgatcagcgc cgcctgcggc cacggcccgc tcgttacggc
    10141 cagtcgcccg cggcggtcca ggtgccgcgg cagtccccag gtgagtgcgc cgcgcttgcg
    10201 ggtcgggcag actgccacac attcgaggca ggccgtgcaa cgcgccgcgg tcacgtttgc
    10261 ctgggtgtgt acgcgaatgc ccatcgggca tacgcggctg caggcgccac aatcggtaca
    10321 cgcgtccggg ctgcgcttga tgcgcatcag tccgaagcgc gagaatggtg tgaacacggc
    10381 cgccagcggg cagaaccagc ggcaaaaggg tactttgaca aacagcgaaa tcagcaggat
    10441 cgcgccgctg ataacgtagg cccagaaagt gatgtcttcg ccgtgccggc tcaataatgc
    10501 gtagcacggg tcgtagccgc ggaaaaccag ttcgccggcc gtccaggtga aatacaggat
    10561 taggcccagc acggcgtatt tcagcagtgc caggccgcgg tccagaccgt atggcacgcg
    10621 ccgcggcctg atgcgcaagc gccgggcggc cgcttgcaac cattcactga tggccccgat
    10681 tgggcagaca tagccgcaga aggcccgccg cagcagcagt accgaaagca gcacgccgcc
    10741 gaggatgtag aagttcgaga ctcccagcga gcagatcagg tttccctcgc gcgcgtaggt
    10801 atacagcgcc tcgacgccgc cgaaggggca ccaggcttcg gcgttgccgc ccacgagaaa
    10861 tactgccacg agtgtcaaga gtagaaagga gaattggact atgcggcggg caaaggtcat
    10921 tgtcaggcgg ttaggtgagg caactgaaca agcaggggac aagcggggcg agggctcact
    10981 ctttccactc tttacagttc accccggggg catgcccgcg ccccgctgcg cggggcttgg
    11041 gcatggcacc catgtcgagg gattgaaagt cgagactcaa acgcgaagct tcctgttatg
    11101 gcttgctggg ggcatttgac ttgccgtggc ccttgcaatc ctcgtgggac ttctggtctt
    11161 tgccctggca tttcttcttc aagggacatt tgccgcaatc cgggcccttg ctgcacttgg
    11221 attcagcaac cttggactgc gtatcgaggg cctttttgaa ttccggcttg ctctggaggg
    11281 gcttcagatc gtcgcaggtc cgcgcctgct ctttgatagc atccagcccg ctgggcggtg
    11341 tggtctcgaa gcagcgcacc agcgccgctg tggcttcctt gtgctggccg agtagagtgt
    11401 gcagccgggc caggtcaaat tcgcactgcg ggctctcggg tgcgtcgtcc ttgagcgtgc
    11461 cgagcagctt cgcggcctca tctttctgac cctggcgcgc cagcgccaga cccagcaaca
    11521 cgcgcacttg ggcgtcgatc tttgactgcg tgatgccgcg caatccctcg gccgcctccg
    11581 aattcatgcc cagcgccagc cgggagcggg ccagcatgac ggccgtttcg gtcgtgttca
    11641 gcttggtgtg agccctattc gccagagcca gagcctggtc atgaatcccg ttctcatcgt
    11701 agaaggtgtg cagggccttg gccgtgttca gccacttttc attgtcggtc tgctcgtcga
    11761 gcgtctggcg catcgcgagc acgcgctgca cgatcgcggc gcgcacctga tagttctgat
    11821 tgtcgggctc cgccttggcc gcggcctcga aagccttggc cgctccgtcg agatcgccct
    11881 tgctgagcag ttcggtgccc tgcgtgaatt gcgcctgacc atcaccgtcg ccggcccagc
    11941 ttgcggccgg gagaattacg cacaacaagc cgaccaacac gcccttaagc ttcattttgt
    12001 tctccttcgt cgtcatggaa actgccgtac cgaccgcacg cctcgcgccg ccggcaaacc
    12061 gggaaaattt caaatcaggg ggccttccgc tccgtgttca agcgtgccaa ctgctcgggc
    12121 gtcagggcca tacggacctg cagcacgcaa tgaatgcggt ccttccattc ctgggctcgc
    12181 agcccggcaa tcttatcggc cagactctcc accagttggg aatcaatctt gtctgccgtg
    12241 agggcttgct cgagctgtgc caatgagtct tccaaaacct tggcctgctg ctcgcgctgc
    12301 cgcagacaga cccctccgcc gcagcaactg aatattttta ctctttgttc ctcagatagt
    12361 tgcagctcgt cggcgagcgg acagttcggc cggtcgagag ttatcggaca gaatccgccg
    12421 gccgcacagg ggctggccga tgtccgccgc tgaccgacca ggaacgcgtt caatgctgcg
    12481 ctgccgagca gcgcgccggt tagaagccat atcaccaagg actgtctcat ggtctcaggc
    12541 gtcccgtaag ttcctcaaac agcacaactt cggggcggcg cgccaatgcg tccagctcgc
    12601 ggtcctgtag tccagcgccc gcgaccgcgc cctggagttc gcgcagggcg agctccacag
    12661 caatgctagc ctcggccgac gctgcgtccg gccgggcggg acgtacgatc agcaggcgtt
    12721 gtgcagtcac gcacaaggcc aagccggctg cggcggcggt tatccgaatt ccccactcgc
    12781 gaattcttcg gcgacggcgc tgggaggcga gcgcgcccag cacgcggggg agcaagttcg
    12841 ccggcacttc ggcgggcgtg cccgactgca acacacggtt cagcagcgaa agtcgcgcaa
    12901 gatacgcgcg gcacgccgga caggtctgca gatgggcgac caggtcttcg cggcgcgcgg
    12961 ggtctaactc gccgtcgtgg taggccgaaa gctgtttttg ggcatcttgg caggtcatct
    13021 ttccttccgt gccgctgttt tgacaccggt gggaattcgt ctattccctt tcgggcgaaa
    13081 aagttttggc gggggcgttg gtatttgctg cctgtcgtga ccgggtcatt tttcggtcgt
    13141 ttcatcgagc atcgcggcca ggagccggcg ggcgcgggag aggcgggaca tgacggtgcc
    13201 gggcggaatg tcgagcaact ccgcgatttc ggcataggag cggccttcga tctcgcgcag
    13261 cacgaggcag gcccgatagg acggcggcag tcgctccagc agcgctgccg cgcgggcgtt
    13321 ttcgtccttt gtcgccggca gccggccggg ctccgcagtc acaagccgaa ttcgctgcgc
    13381 aaccgcagcc ttctccgccg cagcccgtcg ccgccgttgt cgcagcttat tcaagcagta
    13441 ccgcaccgcc aggcggtaga cccacgtcga aaaacgcgcc tggccgtcga attttccggc
    13501 ctgttcatag acgcgcagaa aaatttcctg cgccgcatcc tcggcatcgg cggaattgcc
    13561 cattaaagcc cggctgaggc gaagtaccgc atccgacagc gcccaaaaca ccgcctccag
    13621 cgccgcgatg tccccgctgc gcagccgccc caccgtctcc gcatccggtt cccgacgcgc
    13681 atcagggtcc aagccgacaa ctcctcagct tcgccggatc tctgcactcg cgcgctgcag
    13741 gcaatgccgc tctacggcag gccagttagg accggccgcg gtattattgg ggcaccatga
    13801 gccaagacca agccgcctgc gcgcctccgg tgatcgtcgc ccgcaagctg acgcggaagt
    13861 tcggcaattt ggaggccgtg gccggagtgg atttccaggt tcagcccaac cagtgcatcg
    13921 gctttttagg acccaacgga gccgggaaga ccacgctgat gcgcatgatc tacagtgcga
    13981 tcgagaaaac ctcgggcgag ctgaccgttt tcgggctcac tccgatggtg gatcgcaagc
    14041 tcatcaattc ccgcttgggc gtcgtcttcc aggacaacaa cctggacgag gacctcgacg
    14101 ccgccgaatg cctgcacgtg catgcacttt actgcgggtt ggactggccc acagcccgcc
    14161 gccgcacggc tgagctgctg gagtggctct cgctggccga caaagcccgt agccgcatcc
    14221 gcgccctttc cggcggcatg gtacggcggc tgatggtcgg acgcgcgctg ctgaacgggc
    14281 cggagctgct gattctggac gagcccacga ccggtctgga tccgcaggta cggcacgtca
    14341 tctgggccat gctgcgcgag ctgcgccgcc agggcattac catcctgctc accacgcatt
    14401 acatggacga ggcccagcag ctcagcgaca gcataatgct catggaccgc gggcgcatca
    14461 tactcagcgg gcgcccgcaa gagctgatcg aacgtcacct ggagcggtat gttcttcagg
    14521 tttcgggtct ggagagccgg ccggcgctga gcgccgacgt gcggcacgag catgtcggcg
    14581 acaccgacta cttctattca cacaccgagc cgccgctcca ggcgctatac cgccagttgc
    14641 cgcagaacgc gctgctgcgg cacgccaatc tggaagacgt attcttgaaa ttcacggggc
    14701 gcgggctgca cgagtgaggc gaaagggcaa taccagtgac cgagcgcgaa cgcggccggc
    14761 atgaagagct gattgagcag gagctcgcac gggctgcgca ggcggctcag ccggcggccg
    14821 agccggtgct tggcgtgcgg ccgccgcagt tgggctattt ccgcacgcag acgcggctgc
    14881 ggaccggctg gttctggagc ctatacggtg tgtggtaccg gcatgttcgg gtctacacaa
    14941 agacgctact ggccaatgcc gcaccgccgg tctttgaacc gctgttcttc ttcaccgcga
    15001 tcgccctggg cctcggcagc tacatgccca atgccagctt cgacggtctg ccgtacgcca
    15061 cgttcgtcgc cagcggcctg gtggttgcgt cggccatgtt cacgtccgtc ttcgagacga
    15121 catttggcac atttgtgcgg ctggtttatc agaaaaccta cgacgccatg ctcggcacgc
    15181 atctggcgct gaaccagatc ttcattggcg agctgctgtt tacagccact aagggtgcgg
    15241 tgttttcgag cattgtgctg ggtgtgacgg cggtcttcgg ggtgcggccg acggcctggt
    15301 gcgtgctggt tccgatagtc ggggcggcga cggcgtacct attcggggcg attggcctga
    15361 ttgtcacgag ctatgtgcgg atgatcaaca acttctcttt tttcaccagc gggataatca
    15421 cgccgctgtt tttcttttcc ggcactttct tcccggtgcg tgggcataac gcggtgttga
    15481 cggtatttgc cagtcttctg ccgcttactc cggcgattga actgtcgcgg gctttgtaca
    15541 agggcgagtt tacccgggtc acgttggcgt atctggcggt cttttgcgtc tatattgtca
    15601 tctgccacgt agtgtcgttg cggagaatgc gcaagcgcct gctgtcctga atcaccgcgt
    15661 atgggcgcct gcgcggctat gatccgtggg cccgaaacgc acggtttcgg acccacgggt
    15721 attggcgtgc cgttgtggcg agaccggtac cactgtctgc ccactcaagg gctgacaggc
    15781 tcgacgggat agacgctgct cggagacact gccgctcctc ccaccgtggc acgcgcggag
    15841 agcggcagag ggggcagtgt ggaggcgccc gtggcgtcca tctgccgcgt gccgcttccg
    15901 ccggcggacg aggtcaccgt gacgctgccc ggcggcaccg ccacgtccgg cacgaccagg
    15961 cgccaggagg gggacatcgt gccgtagccg accacggtca gcgtcggccg cggcaaggtg
    16021 tcgctggagg tggcgtctat ggtcagaaca tggttcgttg cgtcgtaaag ggcgcgggtc
    16081 acgtgcacgg cgtcgaccag attggttgtc agcgtggtgg gtagactact cggcgggttg
    16141 gtcccagtga ccgcaacggt ggcgggtaac ggcgtctcgg ccccaaccgg gagccgcgcg
    16201 aagtagttgc cggttccatc ggtgatcatc ggcacatcga cgatggtggg accggcattg
    16261 acgatcagag tggccgtcgg gacggcggtc gcgtatacgt cgatgaactt gccaagtgta
    16321 ttgcggctat acgtcgcacg gtccaggcgc atcggtgtgg gagtctgccc gtcaaagtgc
    16381 tttccgcaaa gctgaaacag ctcggtctgg acgaagttgt tgcctgcgcc gtccaggtcg
    16441 gcgccgggag gcccctcgat gcggaagaag ttggttccaa aggggctgcc ggtgacggtc
    16501 tggtcgacct gcggatttcc gatgtagccg tccggcggcg gcggattaac ggcgcgcagg
    16561 tacggtccga tggcgcctcc caacacgcgt gagaagtcgg gcgtaatgcc acctatgtcg
    16621 gaagtgtagt tgatgccccg gtcccctgcg ggccgattag gaaagaaatt cacgccgtac
    16681 ggatgaatga cggtatagtc gccagctacc ggagtatcga tgcggatccg aacgcgtgcg
    16741 aacacagtct ggtcgccgtc gataggctcc tcagctgcga atgcggcttc cacggcgagg
    16801 acgagccggg cgtcacggtt gccaggcagt gtaatgctcg attccgcgga catatagaag
    16861 gcttcggcgc cgaaaccgac tacggcgctg aacggaaatt cgggatcaac cgggtcgaac
    16921 aaagacagac ctgtagagcc gtctccgtcc agcccgatct gcagggcgac gccgttccag
    16981 tcctgaatcc attgtgggaa acccttgacg gggtcgatgg ggccgacttc cgccagtccc
    17041 ggttgagctt gcgcgaccgt cgctatgagc agcgaggcgg tcattgcggc cacgatgtgt
    17101 aaagtcctag tcattgtgtt ttctcctagt ttggcacccg agcttttacg gtgtaacgac
    17161 aaatggaatc cgggccgtca taagcacgac ttccggcgtc ccgggtactt gaccgcgcgt
    17221 atcgatgaac tgcaccgtgg caaacgagtc gatcggctgt gtcggccgga tcaaagcgtc
    17281 gaagcgccgc gccgtgctga ggtggaaagg cacaccggcc ggcaaaatga aggccgagtt
    17341 gtacagcccg aacggcggca ctcccagggc ccggccgtcc atggcaatga tcactgcgtc
    17401 gacgggcaag gtcacgctga tggagttgta cgcggcgtcg atgcagcgca ccagaatcgt
    17461 ctgaccgacg cgcgcgttga ccgaaatctg cgagccggac acgccgctat tcagctccgg
    17521 cggaatcacc aagcccggcg ggatggctgc ggtgccgccc ttatgggcag gcactgggac
    17581 gcccgtgatg aaccagtaat cggcgttgaa gtcgttgaac gcgaagaaat catcgtggcc
    17641 cgggttccga tcgaactcgt cgtccacgcc gggcgtgctg ccgaacctgg gatacgtggc
    17701 ccgcgcgtcg ggcgccaaat cgctccagac tgagtcgcgg tcgtcgggca cccagagcac
    17761 ttcgacgtcg tacggcacgg taaaagcgtg cgggtcgcca ttggcgaccc cctccaccgg
    17821 gtcgccgccg atgaagcccg gaaactgcgg gaacctggcc aaattggccg cgacacggaa
    17881 tttgccatcc gagcaggccc caatgggtat atcgttcaga tgatacgtgc cgtcggggtc
    17941 aatcgaagcg atggacgcga agtacgcgtc cggcggctcg atcagcaaca agccgtatag
    18001 gccatattcg aaatgttgca cggtgttgcg gtggcagtgg taaaaataag agccgatgaa
    18061 atttggctgc cattggtaga tgtaatgccc gatttccatc gagcagtgac ctacgccatc
    18121 gttcatcgga gttggttcta tgccgtgcca atggatcgtg tgcggcggcg ggccatgacc
    18181 ctgggtttcg cagtgaaaga tgaccccgcg cggcacgcgt atggtcgcgc ccgggaaatg
    18241 accgtcgtcg gccgggttgt cccgatccct gaacagaaag aacaccaatt ccttgagacc
    18301 gtcccacgta tcgatgtcgt cgccgtgcat cagatcgcgt tggacggtat gaggcgtggt
    18361 cacgcggtcc aggtatccgg ccgcaagcat gacttccggg accgggttgt tcggctctac
    18421 aaattccggc gtgtccgggc tttcattcgg cgggtcgaac atcttcgccc aatgcccctc
    18481 cattccaatc actgactgcc gcggtatatg aaaagctggc atcttcatct ccattctggc
    18541 ataacgttct caaagtgcat caccacttgg ccgtcccctg ggggtactcc ccgattggcg
    18601 gcgccgccgg tccggtggcc tcggggccgt gcccctcgtg ctcgcttccg atggggaaag
    18661 tcgtgacacc gctgggagtg ttacgatcgc cggtgaagtc gatcccgctg accagcccgc
    18721 agttgtagtt gccgccctga gacgtttggg tgggttcgga gtggtcgtgc attgggtagc
    18781 acaagggcga cagctgcacg cttagatcga ttggctcgcc ggccaggtcc agccccatgc
    18841 ggtcgccaat ggccgggatg aagagggcca attcttcgtt gggcggccac gtggttccgc
    18901 cgccggtcgt gggaagcccc ggatcagccc ggccgattcc gcgttcgttg ggaatatccg
    18961 gcggccgcat gaacggaacg gcccagtcga acgtatccat ggggcgtgcg gtgaacgtgt
    19021 ctacccatag ggcattcttc tgaacggcgc cattaagggc cagcaggtaa acatgattgc
    19081 cgtggatgtg tatagaatgc gtccacaacc cagcattaag aacgcgaatt atcaccggct
    19141 cgcccacgcg gttgttcggg caaagatgcg ggctgttatg cgcgaagtgc ccggactggc
    19201 cgttaatcgt gaaatactgc ggtattcgat tatcgccggt cgggctgaat gggtcgcgca
    19261 agaaggcctc catgaactga tccccagggt atatctcccc tggcggcagc tcgccgacct
    19321 cggcgaacag attcgggctg gcttggtgca acagccagac gtattgcctg aaagccagag
    19381 tgtgggtgtc gtggtcacct tcttcccagg ccaggcccgg ccaccacgga gcgcttccga
    19441 agtcatcgaa cagccgctgc accgcggcgg tcggaacgac atacggcgtg tatttgtgcc
    19501 ccggctgcgg cgccgccggc atgacgatga aagccccgtg cagccccatc attcggttaa
    19561 ccggctcatt caggttgtcg tagtagagga aagtgccggc tttggaagcc tggaaggagc
    19621 ccttgtacgt ctcgcccggc tgaatcggcc cgctgttgaa catgcccgga atgtagaacg
    19681 cgtgcggctc gtcgagatcg ttggtaatgg taatttcgat tgtttcacgg taggtcgtga
    19741 ggataatggg gccggggacc tcggccggcc agcgctcttc tttgaaaacc cagaagtagc
    19801 actgggcttc gttgatggca ttgtgcgtca ccatgtcctt tatggcgtcg ctgatgtgaa
    19861 agttgagcgt ttgtaccgac agggaagcgt agtggggtag aaatggcatt ttctgggcgg
    19921 ccgatccgac caccagcgcc gccgaaccgc ccaggccgcg cgccaggaat tcgcgtctgc
    19981 ttaaagccat tgatggttcc ttttttaaat gcacacatgc gcaagctcgc cggcccgcgc
    20041 agacgttact acggctgaaa aggactgagg ttgaggaacg tgcctagcgg cggcagcagc
    20101 gcttccacgc gcccgcccac gcccaccgcc ggtatttcca ggacctcttc attttcacgg
    20161 aatccgtcgc gaagtacgcg gctggagccc acaattgcgc tttcatctcc gaattgccca
    20221 atcggcacga agagctgcgg gtggtcgaat ggcgcttgtt cctgacgaac gcgctcatcg
    20281 gtcagcgaca atagaaagct caccagggcg ttcttgtcat cctcgctgag ttccaattcg
    20341 ccgatgaacg catgcatgtt gtcgaagttc tcgtcgaaga agtcgccgcc gcggtcataa
    20401 aatccacgac ctgccgcagc gttgcctgtc cgccgttatg gaagtagggg ccggtaagct
    20461 ccacgttccg caggcccggc accttaagtg acccatcgac tgagacacga ttcggatccg
    20521 gcgaacccgt tccgaagggc aggtccggca cgaacagcga caccgccggc ggtaattggc
    20581 cggcgcgttt gagcattgct aatcgtgtca gcgacagtgg ataaggttgc ccagtcacgg
    20641 gattgatgaa gggcgcagtg ccgccctgcc cgatatcctc ggccgtcggg cgtacgccaa
    20701 tgttaatgaa acccctgtcg tagaaagcgc ggccgggact ggccatgaac atgagctcga
    20761 tgccgcgcag ggccgcttcg gtcaattcgg gtccggtgtg acactgtgca cagcgcccgc
    20821 ggtcgaggaa aatatcgaag ccggccagtt gttccgccgt cagcgcgtcc gggtcaccgt
    20881 ccaggtaccg atcgaaaggc gtgtcatccg ccaccagtgt ggactcgtag gtctggattg
    20941 ccagtccgaa gaagaacgag aagttggcct ccatttgggt gaattggttg ccggccaaca
    21001 ttcccgccgg acgaggtatt acgctcgagc caccgtccgg ctggtattct attatgaggt
    21061 cggaattcca gtattgcggt tgaaacgcgt cacggatcaa ggcttcgtac gttgtaaaga
    21121 gtccgcgctg gcctgcgagc ggcacgcccc caagactcac cggggagccg gaagtctccg
    21181 gcacagtgtc gtcggatgct gaaactttac tgtccagcgg gcgcgccagt cgcgcccggg
    21241 ataacggtcc cagcacgctg tcgcgcgggt ggaccatctg cttgtctagc ggacgcaggt
    21301 tcagcatttt gcgtccgatt tgcggccagc tccgtccagc gaacgacatt tccgtggagg
    21361 ctagcggggg cgctaccgcc tgcgaggcca ggctggcttg cgaaatcagc aggggctgct
    21421 tgccgaggac gccctcgata tttacgaaaa ctcctgagtt cgggtccagg gggcctgaga
    21481 aacttacgcc gttgaagaag tgactggccc gcccgtccca gaaattggca gcgttgaaga
    21541 ctgcattaat gaccgtcggc gtatttctga ataacacttg ccgcgtgttg acggcgccta
    21601 cgtgaaagat cgggtccgcg acttcggtgc ccaaatccag gggaaacccc agccgcgtct
    21661 ggatgaagtc ggtttggaac tgacccaagg acgagacgat atcgttgctc tcccacagcg
    21721 gtggcgagtc acggtcctcg gggtccgaga gggcacgcag cgggaaatcg gtaaagtgca
    21781 gggtgtagtt cggaccggct acttggaacg tctcgtcgcc ggcgcgtatt cccggcgaga
    21841 gtgcgttctt gatccggtta tctgccccgg cgtggaagtg gcaactggcg caggccgtaa
    21901 ctccgtcact gccgacctgc atatcccaga atagggcttt gcccaactga atagccgcct
    21961 gacgattccg cacataaagg tgcaggttcg gcggttcggg cacgatgacg gtcttcagcg
    22021 agggcggggg cagagctggt tcagccggag ccggcggttg ggcctgggcc ggcagggcgc
    22081 caggccccac taccactgtg attaagagca gcagctccac ggtgcaacag gttctgtaat
    22141 cgacaccgga ggctgagtac ttagctctgc gtctcacgct accctccgcg agcagtagct
    22201 cgcgcgtgcc ttacggcaca caacctctaa gtcgtcgttg cagcagtatt gcggcgcaca
    22261 atgccagaca taccagtgag gccggctcag gaataaggtt ctcattaagc gtcagcgagg
    22321 ccacctctcc cacgccgaga gtcaaatccc actggaacgc gaaagtcaga tcgcccgcga
    22381 gcgggccggt aaagtcggag agcgtcgtcg ggctcaaatc gctcagcgca tcgagcagct
    22441 ccgggtagaa ggccgcctgc cggtgactgg agagcaggtc gctcacgcca cggtagcgat
    22501 actcccactg gggccgattt ggcgcgccaa atcgttggtc aggaaggaca aggagttgaa
    22561 gtcaaactcc ccgtcaacgt ccagatcaac atattgaaac aggtgcaagt ccagcggtac
    22621 gtccgccaca ttcgtaaggg tgatgatgtg cgcaagctga gactggccgc tgcccagcgc
    22681 tccgcccaag agcgcgtagt tcaagtctac atcaaacagt ccctgagcga attgcacggt
    22741 cgccgattga ccatcggtag taaacccaac cggttcaacg gagctgatag ggttctcctc
    22801 gttgtcgccg atccggaacc agagttgctg agcactcagg tgttccacgc catcgacggt
    22861 ccatgagtac agtccgagcg cggaaccggt atcgattcgc gccacggagt tgccgctgct
    22921 caaggtaaca agctccgcta cagcgggccc ggtcacgccg ccacaatagc acgtcactac
    22981 cgcgatcaag agaatgcgct gtgtcatgaa tatcgctcct ttccctgcct tgctctggaa
    23041 agtgccgccc gacccgccac gcgacgcgtc aggaccggcc cgctcctgtc aaggaatcac
    23101 ctgttccgca agtccaaccc ggacgcgcta taaccgtgaa gcctcaaagt tcccgaggct
    23161 tcagcgcttg cccggcctcg ttcatgcttg ctatgtgtaa tacacgctac agttgcaaat
    23221 cgctgactag cgtttcaagc cggcgagcac gcagcgctgt gagctgctgc acttgttgat
    23281 agccggcgta atatcgaagt caagaagtag cgagattgaa tgctggcaat tcagcgtttt
    23341 gaagtgcttg tggtaaccta tagacattgc cggcgctggg cgactgtgcc gcttcccgat
    23401 tcggcactca tgctctgcgc ttctgctcaa ggcccgcggg gaattaaccc gggccgatca
    23461 cttcaacccc gttgagatac gggcgcaagc gctcgggtat ccggacgccg ccttgcggag
    23521 tttgatacat ctccataatt ggaatcaaaa ttcgcggcga ggcgatcacc gtattgttga
    23581 gtgtgtggca gaactccggc ttgcccgtgt cggctctgcg atacctgatg ttcagccggc
    23641 gggcctggaa atcatggaag cggctggccg agtgcgtctc ggcgtagccg tcgcggctgg
    23701 gcatccaggt ttcgatgtcg tacatccgga ctttgccctg tcccatatcg cccgtgcaga
    23761 cctcgaccac ccggtaaggc agctccagcg cctgtagtac cctttccgaa ttggaaatga
    23821 tgacatcatg ccagtaggcc gattcgtccg cgtcggcttg gcagacgaca acctgctcga
    23881 ccttgtcgaa gtagtgaatg cgatacagcc cgcgcgtgtc cttgccgccg gcgcctgcct
    23941 cgcgccggaa gcaggtgctg cgcgcgaaat acagctttgg caactccgcc tggtccagaa
    24001 tctcgtccga atgcaagccg gttaccggca cctccgccgt gccgaccaag ctctgcccgt
    24061 cgcgctcgca caggtatact tcctcgcggt gcagcgggaa gaagcccgtg ccatacatca
    24121 ggctatcgtt tacgagtacc ggcacagtga gcggcttgaa ttgcagcttg acatccggcg
    24181 cgcccggctt gtcgaagcgg ccggccagca tgacgtccca cgccagccgc agcaccgctt
    24241 cgtgcagcat cgcgcccagg cctgttagaa cgtaatttcg cgagccggcg atcttcaccc
    24301 cacggtcgat gtcaaacagc ccaagctgct cgcccaactc cacgtgatcg cggaacttcg
    24361 gctcgaacgc ctcttttgcc ttcggcgcgc cgacgcggcg gacctcgacg ttgccgctct
    24421 catccggtcc gatgggcgcg gccggatgcg ggatttgcgg cacgagcaac atcagctcgt
    24481 tcagttcggc atcgactttt tcgcgctcgg cgtcgatctc cttgaggcgg gccttgatct
    24541 cacgcatctc gttgctgagg gcggcctttt cctctttcgg cgccttggcg atccgctccc
    24601 cggtggtgtt ctgctgatgc cgcagcgtgt tgaactcagt ttcgagcgcc cggcggcgcg
    24661 catcgatggc gataatctgg tcgatgtcga cgcgcatgtt cttgtcgcgg acggctttct
    24721 tcaccaggtc cgggttttcg cgtatcagct tgaggtcgat catgactgcc tccaggcgcg
    24781 tgcggctgca agcgcgaagc ggaaattata ttgtcggcac gtctgctcgc gagtaccgga
    24841 ggttcaatgt tcgacaatcg cggcgacgac cgatcacttt cggccgcgga tgacgtggcg
    24901 cactgtaagc cgagcgttat gcagtggcca atgaccacgc gcgcaccgcc gactgataac
    24961 tttcctgtta agcgccctcc ggcaacgctg cagacgtcgg cctcccgtgt tacgatagcg
    25021 cttgcgccgt tgggcaacgc accgggcgac atccctccgg tgcatgccgg ctgaacagcg
    25081 cgatggtgag cggaggttaa atggctggtc tcaaggctgc tccaggggac gcccacagca
    25141 tcgcggccaa ttacgtgaac tcagccgtgg gcgttggtct cctcccccag ggctgccagt
    25201 cggcgaccgc cgcgaatccc cgatccttcc agcacgcact gtgcgtctat ccgtatcggc
    25261 gggaactcaa cgaggcgggt ttctttccgc cgctgggatt ggaactcata gcggccgtcg
    25321 tacaacagta cgcgcgctca ctggacctca ttgatttgcg catggtggcc aaccggacgc
    25381 gaagttacct gcggccacag acggatctgg tgtgcttctc catcaactgg gatcgagatc
    25441 gcgaattcct ggaagaggag attcgttccg tgccgcccgg aattctcacg gtgatcggcg
    25501 gccggcacgc cacggaaaac ccgggccggt ggctggcgga ttttccgaac gtcgacctgg
    25561 ttattcgcgg cgatggcgaa gatgcgctgg acgaagtgtg ccgcggggtc cccttgaatc
    25621 agatcgccgg gctgagcttt cgccaaaacg gccttattgt tcacaatccc aatcgcagtc
    25681 tggggcatgt gcgcgacgac ctctatccgg atcgggaggc tcgccgccag gactacgtcg
    25741 ttaccatcaa gggcgctcgc gccggcatcg cggtcgactc catcgcaacc tcgcgcggct
    25801 gtcccaacca ctgcgccttc tgttcgttca atcgcaatcc gtggggtgag atgcgccgct
    25861 ggtcggcgcg ctcgccggag tccgtggtcg atgagctggg gagaatacag gcgcgcctga
    25921 tcgggttcgt ggacgaccta ttcacgcacg acctggagcg cgtggagcgc atctgtgacc
    25981 tgatcctggc gcgcggcatt cgcaagcgct acttcgtgaa cgcgcgcctg gagatcgccc
    26041 ggcgcccgga cgtattacgc aagatggaac aggccggctt ctccatgctg atgctgggtg
    26101 ttgagtcggc tcacgacaag accttgcggt cgatgcgcaa gggcttcgac gtggcgcgga
    26161 tccgcaagta ttttgaagtg ctgcgccaca gccgcatgtt tttgcacggc tacttcatcg
    26221 tcggctgtgt tggcgagagc cgcgaggaca tgctgcggat cgcaccgttc gcgcgcgagc
    26281 tgggtctgga cacgatcgct ctctcggtgt tgcgctccag cccgtattcg ggtctggacg
    26341 agctggtggc gagcagcccc ggctaccaca tagcccccaa cggcaagatc tactccgacc
    26401 agtgctcggt caaagagctc ggtgccctgc gccgccggat ataccaccag ttctacacgc
    26461 cggggcagat tgcccgcgtc actctgaagg cattgcgaaa cgacgcttta agcttccttc
    26521 cgggtttcct gccacatctt ccgcaggtca gcgtggcggc gctcagccac gcctggcgca
    26581 aagctcgtga ccgccggcgg aagcggcgcc tccgcagcgt tgcccagcct gctgagtagc
    26641 cgacgttccg ccggccgcag gccaagcggc ttcgaccgcg cccaacagat cctcgcgcga
    26701 aaagtcaccg atcgcgacaa tttcgacttg ctcgatattg tgcaggcttg ccaaagcctc
    26761 acgcacttcg tcggccgatc ctgggtactc aggcgcggag tgcgccgcgc tgatgaatcc
    26821 acttgggacg taggccgcaa cttcgccggc ctcgatcgcg agccggagcg actcggcata
    26881 tttttgtcgc aattgggctg cccgcgcgca cgcttgcggc gatgtttgcg gccggcggag
    26941 caattcggcc tgaagctcga tgagctgcgg agcatactca gacggcccaa aggcgagtag
    27001 cccggcctgc cccgaaagct ggg
//
LOCUS       DAOSYS010000012        26832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1482_length_26832_cov_5.648131, whole genome shotgun sequence.
ACCESSION   DAOSYS010000012 DAOSYS010000000
VERSION     DAOSYS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26832
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_26832_cov_5.648131"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1775)
                     /gene="thiC"
                     /locus_tag="PLU99_01525"
     CDS             complement(201..1775)
                     /gene="thiC"
                     /locus_tag="PLU99_01525"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOO15763.1"
                     /translation="MTQLEAARKGVITPEMRRVACRESVTPEFIRDQVATGRIIIPAN
                     LRHLAGSGGEAPAADSAATRPASAEQQPVGHPGCAPESMLWVNQTVAQRRQLLADPEY
                     CRGERAPKRLDPMGVGRAISTKINANIGASPVASDTASEVEKLRWAQLYGADALMDLS
                     TGGELDCCRQAIIDNSTIPIGTVPIYSMIVGRTIEELDYDTILRTVTRQAEQGVDFFT
                     IHAGILREHLGLIRRRTTGLVSRGGSLIARWMTYHGRQNPFYELFDEISAIMREHDVA
                     YSLGDGVRPGCLADASDPAQIAELHVLGELVQRARAAGVQVMVEGPGHVPMNEVAWNM
                     EKQRAVCDDAPFYVLGPVVSDVFPGYDHITSAIGATEAARAGAALLCYVTPREHVGLP
                     KAADVKAGCIAYKIAAHAGDVARGIRGARQWDDDMSRARAALDWARQFELAFDGETAR
                     ALHDEDIQTDTDYCSMCGRDWCSLRISRDLKSNGPAEGQMAGTADPAACPAGKISRTL
                     DPAVCPAARTTQPDEP"
     gene            complement(1983..2753)
                     /gene="thyX"
                     /locus_tag="PLU99_01530"
     CDS             complement(1983..2753)
                     /gene="thyX"
                     /locus_tag="PLU99_01530"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOO15764.1"
                     /translation="MQIIREPRVYIVGRVAANSAEIERFLKEHGLSWSTDTEVGAEAL
                     CEVAGRLCYMSFGQGRKTNAEYLANIIESQHGSVLEHVAWNLLITGVSRSFTHELVRH
                     RAGFGYSQLSQRYVDESTADFVEPDVIAEDPELHKVWLESIQQAHAAYCKLVEGLAGK
                     FPNEPSATMRRKLARQAARSVLPNATETKIFVTGNGRAWRHFIEMRANEHAEVEIRKV
                     ALQILRLLQQEAPHLFGDYEVVRLADGTQVARTAHRKV"
     gene            complement(3020..3541)
                     /locus_tag="PLU99_01535"
     CDS             complement(3020..3541)
                     /locus_tag="PLU99_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008570066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO15765.1"
                     /translation="MHIWTIGHSNHPLSVFIDMLTAHAIHTVADIRSFPGSRKYPWFN
                     SGPLSATLRAADIEYESLRELGGRRRPSRQSVNAAWRNASFRGYADYMQTPEFHAGIE
                     RLLALAAAHPTVIMCAEALWWRCHRALVSDYLKVSGIQVWHILSAGKTQEHPYTSAAR
                     IVRGVLTYPGDAN"
     gene            3699..4616
                     /gene="ftsY"
                     /locus_tag="PLU99_01540"
     CDS             3699..4616
                     /gene="ftsY"
                     /locus_tag="PLU99_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOO15766.1"
                     /translation="MGLFDVFKKGLQKTREKISAGLRTILPFGRKIDEQLLDDLADAM
                     LTGDMGPRAVSGLIGEVRAAWKAGQLTEAQEILPFLKRRIAETWKPGDRALRLAPSPP
                     TVVFVVGVNGSGKTTSVAKLAAWLRKQGKTVLLGACDTFRAAAIDQLVIWAKRAGVDI
                     VKHQPNSDPGAVAFDACEAALARKVDVLLIDTAGRLHTQDHLMRELGKIKRVVQKRIP
                     DAPHEVILVLDATIGQNAINQAKVFSEVTNVTGLFLAKLDGSAKGGIVVGIRDQLDIP
                     VKFVGLGETIDAIEPFDPETFAEALFAEA"
     gene            4862..5611
                     /locus_tag="PLU99_01545"
     CDS             4862..5611
                     /locus_tag="PLU99_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15767.1"
                     /translation="MIESRYPVPEGQAVRPAEPGVQPETRPREFQPETRPREFKTTAG
                     VVAGGSAIETLAGAAAVVLTIIGLAGWYPALLLPIAAICVGAGLMLKGGALAARSHFL
                     MREVSGESMTMAELGGGMSAELMGGAAGIVLGILALAGIAPSTLLSVAAIVFGGTLMF
                     GTGAEAPLARLTAHPAVHPAVHAAARDMAHASASSQFLVGAGAVVLGILALIGFVPQT
                     LQLVAILAVGGAAFLAGAVASGRMASVFRSA"
     gene            complement(5770..8112)
                     /locus_tag="PLU99_01550"
     CDS             complement(5770..8112)
                     /locus_tag="PLU99_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO15768.1"
                     /translation="MHAHLEPSAILADLNEPQRQAVTHGAGPLLVLAGPGSGKTRVIT
                     RRAAYLVGTGVPARNILAITFTNKAADEMKRRIEGLGVARGMWVHTFHALGVRLLREF
                     GELVGVLPGFSIYDEADRLRVVKEAMQACGVRDVLLRPEAVQDEISNAKNKLQSPAEF
                     AGWADTFEQRAVARVYEKYEELLRERNGVDFDDLLMRVALLLRGHADIVERLNIRFQH
                     VLVDEYQDTNHAQYLIARYLSERHRNICVTGDPDQSIYGWRGADIKNILEFERDYPDA
                     VVVRLEQNYRSTGMILQAASAIIANNRRRKHKDLWTENQPGEPVYVWRFSEGQDEAEQ
                     IADTIQRLHQGGQAYSDVAIFYRVNAISRGLEESLRIRGIPYRIARGVEFYNRKEIKD
                     TLGYLRILINPADQVALLRIINTPARGIGKTTIERLLATAERQHRPLLEVLRDRGVQA
                     EMKGAGKKLAAFVKLLDQIEQAARGPVSGVVSAVLSLSGLEEALTKEAEEGGEDRLAN
                     VQELVTAARRYEEEVEEPTLADFLYRIALVSDQDQVDEKAGAVMLMTLHAAKGLEFPV
                     VFIVGLEQGLLPHENALMQSLDVEEERRLCFVGITRARQRLFLTHARQRMIRGVTLPR
                     AASQFLRELDQSAARFEDYSREPWDSGGGRAQAEEEQADPDGFVALHDHLPPEEGQRP
                     LRPRAGGGSKWNHQRRRSSEFGDEDDVRIINAAGEALQDTSVYADWKPGTLVRHARYG
                     VGQIVWIRPGSGQTRAAIRFARHGEKTFILEMAPIEKLQR"
     gene            8992..9750
                     /locus_tag="PLU99_01555"
     CDS             8992..9750
                     /locus_tag="PLU99_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO15769.1"
                     /translation="MGAATASAPCVPEGYWRADIWHQMGHEALELATHGFWRGFKVSF
                     WDVENALVLTAAMGASITIRETGVDDAIRRRTKGENELGSMDETIQILGNPGTHFAGA
                     GVLWLASALTKDVREHEVAKSLVEALAVNGVSTILLKVATNTRAPDGEDYAWPSGHAS
                     SSFCTAAVLNEYYGPWVGIPSFALAGLVGYQRLDSRVHDFSDVVFGSFMGYVIGSSIA
                     KDNKMELPELFGMKVIPFADPQTGASGLALFKQW"
     gene            complement(11271..12374)
                     /locus_tag="PLU99_01560"
     CDS             complement(11271..12374)
                     /locus_tag="PLU99_01560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO15770.1"
                     /translation="MTVSVPFLDLKAQYATIAGEIRAALDEVLANTAFVLGPAVQRFE
                     KAFAEYCGVEHCVALNSGTSALHVALLCLDIGPGDEVIVPAMSFVATAWPVLYVGARP
                     VFVDVDPRRYTLDPNQLARAITKKTKAIIPVHLYGQCADMDPILAIAAERGVPVIEDA
                     AQAHGATYKGRRAGALGRLACFSFYPGKNLGAYGEGGALVTHDAELADLARQLRDHGQ
                     KTRYVHERLGFNYRMDGFQGAVLGVKLKYLDRWNAARRRVAAEYDRKLASAGVITPPP
                     CPDGEHVYHIYALRCEKRDELKANLQECGIGTNLHYPTPIHLQKPFEPFGYRAGSMPV
                     SEEIGATELSLPMYAELTAEQIERVIAAVHAAV"
     gene            complement(12414..13622)
                     /locus_tag="PLU99_01565"
     CDS             complement(12414..13622)
                     /locus_tag="PLU99_01565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO15771.1"
                     /translation="MRVLFFSNWFPPVISGSSFYTSSLAQALVERGHEVEVVTVDWGP
                     EYAPAAPFPVHRLPCLRLPRTALFYNARLMGFTFTPANRKRLLQIIARFRPHVLHHVN
                     HIFDSVLLTASAARAARVPLVGSLTTPVQHEKPLRNLVMALGDRVALGWGGVRRWDGI
                     VSLDQMVHHYVGVRFGRRAQQRSEIILFGVRREALAEYQNRTVARAAEPLILFVGQIH
                     PFRNPVQIIRAMPLVLQAVPQARLVLAGRVDLKEPPEVAHALGLSQHVQFLGETAHDQ
                     VVRLMQQAHVFVSYATGPFPGLGTAPMEAMLCETPVVNDLPENLFGEGRLRNGENIIL
                     VNQHDPHSIAGALVKLLRDAELRQRIGAAGRRFVLEHLSWESIAAQMEAFYERVQARY
                     KGTARVGGLP"
     gene            complement(13645..14844)
                     /locus_tag="PLU99_01570"
     CDS             complement(13645..14844)
                     /locus_tag="PLU99_01570"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO15772.1"
                     /translation="MSSDLPLIPVAKPWMDEGEAAAVRRPILSGWVTQGPEVAAFERE
                     FAAAVGAPHACAVANCTVALHLALRAAGVRAGDEVITVSHSYIATANSIRYTGATPVF
                     VDIEPGTFNMSPALVEQAITPRTRAILCVHQMGMPCDLAALLTIARRHNLPLIEDAAC
                     ASGSEILWEGRWERIGKPHGLIACFSFHPRKVITTGDGGMLTTADAKLDAQFRLWRQH
                     AMSVPDTVRHGANQVIFESYPTLGYNYRMTDIQAAVGREQLKRLPAIIARRRELAAGY
                     VERLSGVPGLRLPVEPEWARSNWQSYCVRLPAGCDQRRVMQRMLDARVATRRGIMCAH
                     REPAYREEAWSCGAGPGPCGCEPGRCRRLAESELAQDTAILLPLYHHMTEADQDRVCA
                     ALSAAVS"
     gene            complement(14847..15899)
                     /locus_tag="PLU99_01575"
     CDS             complement(14847..15899)
                     /locus_tag="PLU99_01575"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOO15773.1"
                     /translation="MDLAGINPSVRVGRNVHISAEHVQLGDDVRIGDDVRIECRRLVL
                     RHGARLEARCVVGGMRGPADFIDIGEQSLIAHDCKLLCPAAVLGDYVAIHNHTLMNGR
                     GPLRIGHNCWIGQHCILNSEASLSIGNSVCVGTNTNLYSHGYFGDLLEGCQVFKVVPV
                     TIEDDAWIMGSYTSVAPGVRVGEKALVLAGSCVTKDVPPNHCFGGTPARDMTDRLVPY
                     RHVTPDEKLARIGEFLAEYVQTVFPAAFETEPNGYVVRAPFGPFRLRWQPDVDSAACL
                     ASDIPLLVFTRRNEAEAPPDLVTVFDLTRRQYTRCRTPAEISTISFLKSYRARFVPAD
                     RPRVELPPEYRKLGKP"
     gene            complement(15899..16912)
                     /locus_tag="PLU99_01580"
     CDS             complement(15899..16912)
                     /locus_tag="PLU99_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO15774.1"
                     /translation="MRGKTVLVTGGCGLIGSTIIDQLVRDEGVRTVRIIDNLTRGTLL
                     NIQWAQAHGDVQLIRQDIRNFAHIRPHFEGVDVVFHQAAIRITACAEQWRECLDVLVT
                     GTFNVVEACVQAGVQRLVAASSASVYGLADTFPTDERHHLYNNRTWYGAAKIANEALY
                     RAFNDMTGLPYVALRYFNVYGPRMDVFGKYTEVLIRWLDCLDNGIPPKIFGDGHQTMD
                     FIFSEDVARANVLAAKSDVSDEVFNVASGQETSLRGLLETLLKVTGRTDVQPEFLPER
                     KVNPVPRRLASTEKARRLLGFQAQVGLEEGLRRLVAWRREITPAHMREYDPAQTPCST
                     QRK"
     gene            complement(16932..17417)
                     /locus_tag="PLU99_01585"
     CDS             complement(16932..17417)
                     /locus_tag="PLU99_01585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOO15775.1"
                     /translation="MEFCRIAKDVVLGRDVKIHCFVNLYGCTIGDESRVGAFVEIQKN
                     ATIGRRCKISSHTFICEGVHIEDEVFVGHGVLFINDRYPRATTPGGRPQSEADWKVVP
                     THVGRGASLGSGAVIMCGVKIGAQAIVGAGAVVTHDVPPGAVVAGVPARLQRPRAPDL
                     E"
     gene            complement(17454..18485)
                     /locus_tag="PLU99_01590"
     CDS             complement(17454..18485)
                     /locus_tag="PLU99_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO15776.1"
                     /translation="MSQSTDKQQTIRLGLMGYGYWGPNLLRNFAGSPGAMPVMLADPQ
                     PARLAKAKAMYPALETATDADLLLSNESIDAVVIATPVSTHFALAKRALECGKHVLLE
                     KPMTATVAEAEELVRLAAKRDLRLMVDHTFLYNPAIRTTRELIGSGRLGDVLYYDSTR
                     INLGLFQHDVSVVWDLGPHDFAIMDYLVGKQPTSMTALGSAFGHEQASVAFIVAKFAD
                     QTLAHFHLNWMSPTKVRRIIVGGTQKMVVYDMALPEEQVKVYDKGVDFIEREGVHKTL
                     VQYRIGDMYAPQIPGVEPLSLMCREFLDAIREHRAPLTDGQAGLRVVKLLTAAERSLQ
                     REGSPVLLM"
     gene            complement(18482..19579)
                     /locus_tag="PLU99_01595"
     CDS             complement(18482..19579)
                     /locus_tag="PLU99_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOO15777.1"
                     /translation="MTAVGLSTAGVFGWLAVRKVDWPAAGAALVGANWRLLGLCVPLL
                     CSSVLWRALRWRVVLAQQGAARIGPLALAAGIGQGANAILPGKLGEAVGAHALGRLAD
                     LSRIQSLGIMVVTRLTDAVILFALVLGATWFLPSPALRALRGASLIAVATASLALLLP
                     LVLRRQWGVRCLNYAADILQRRLGTGALEVMRKFGRGLTTAGRAPLLLAFLASTALMW
                     GVLSLSLLIALRAFSIELPAGYAPLIMGLIAASTILPAAPGNIGTVHYFGLVALGLVG
                     VAPGLAGACIIAYHALDLVCALVIGAICWTIAGGRLSLWRKPELDVLARASAPVDGLT
                     FGAAALELTAPEQSQPQHRQSTTALLAAEEA"
     gene            complement(19719..22034)
                     /locus_tag="PLU99_01600"
     CDS             complement(19719..22034)
                     /locus_tag="PLU99_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15778.1"
                     /translation="MFRNDRKVAVAAIAGATAVRLAVLTSTVEPENVLGSLLLTATVY
                     SLAAAVALWAGRRRDELWSLTTIAAGAILVAVAAACLPAQQPLILTREADTEGRRWVR
                     PFTATTGGQSRTAVELQRGEATAGASDRVFADLSRLPNCEHALSVWIARPFPPEPHTS
                     DSAANANPVSVFIRASGQPDGPALTQFTFPAQSNSGHAQWHRLAARIPAEARELVIEV
                     PAGPGLPLPSDPVWVDVQPPRPVRLGLVWPAGMIRLVRALAGASVFFAIAVIPLLLVT
                     PRTRSTFTPGPRGQAWTAGVGAAVAVVVLHLPVLMTTYGMSDDYSCLYMAHHNPEVSR
                     SVLFGCGRFILGLLYPAVFAQIDTIAAFSYLRAAAVLGMAGLGAALATFVLRRGWTGP
                     QAVALAFLICSTPSAALAVGWTQLFALSWALLITWLAFWFQQQMTWKPLWKGIVCGLG
                     AIASLSATFWMYQPCAMFYLVWLILDQLPTRTVEPRRKIATGIRGLLIFAAGALLPYL
                     SIVLQSAPNPRASAVTNVGAKLQWFIQEPLRNASALFELAPGTTVSTIVVVIVILAII
                     CYGWRREWRSVTALVGLLAAAPIVYLPNLMIADNWAAYRSLFSLAALLVVLAAFALRG
                     VVPHAGILSALMVLVAARAATVCSEQTRRGIIAPQALELRLTQQILAQSVAQNINGIH
                     VIQPHWAHCAAERVLYDEFGVATTLHPWSPPHFIWAATRDGALPQLRGMRDLPVTHSP
                     DRPTKQRRGELQIDLHELGDWRAAAEWFAPK"
     gene            complement(22010..25675)
                     /locus_tag="PLU99_01605"
     CDS             complement(22010..25675)
                     /locus_tag="PLU99_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO15779.1"
                     /translation="MSSEAGTASAGSTRDSPGLHGVPNFGYAPADLAAPPAVSIVTTV
                     RHGGATLQRTASSLFGQSFQAWEWLLILERAPDPGAQIILDQYRQGDPRVRVLDLPQG
                     GGTGARAAISAAARGAYVWQLDEGAEIEPTALEELYWFFETHVEHADDGCHAPAGCHA
                     QAPSAGMPAGCHAQAPSAGMPAPSAGPPGAMTSAPPKRWYSLKKGLPEGPRRAPEPFE
                     SLAPAPTGLNRLTRSAPRLLMIIPRLSLGGAERAALELLKQLGQRGWEVTVAALHGAD
                     PRWTGEFTRFTPDVLVLPKFLRLPAYPAFLHYLIASRQPQFVLTSGSELGYLLLPYLC
                     STCPEPVYLDLCHSEAPHWKNGGYARYAAGYQDYVDLTLVTSEHLKRYIVQRGGDAAR
                     IEVLYLNIDTELWRPCPQTRQRVRCELALAPDEVCILFAGRLDAEKQPRVLAATLHKL
                     SARKQPFRALLAGDGAERGWVEQFIRQHGLTERVRLLGEVPAERLRELLAAADIFFLP
                     SLWEGIALTIYEAMACGLAVVGAEVGGQRELVTPECGMLLPKRGEAAEAAAYAAVLEE
                     LIQDERRRQTLGRSARQRIEAHFTLRAMAERFFKLLERGRTLRNRTNRPVLPPAAARE
                     SAAQAIEFVRVNEALDASWREAQSIWRDYQRLSAEQTGGGAAAAFLERAGLTVGSEKI
                     SDDERLRRAEQAGIAVCAELNRVSAELAGIYNSTGWRLLQTLYRLRFFVFPHGSWRER
                     LGRRCMHALRQLRQRLHSDRSLARTLPHASREPFTERECKAPALPKSESYSCRRPLRV
                     LLQVEHFEQGGLEQVMLDLAATLSAAQMTVGLLVLGRQGTAAFEARRAGLPILHLPQL
                     GRAQAYRDLLRDWDLVNAHHSLFGVELVAEAGRPFVQTVHSAYVGLDADMRAAYRRAD
                     AATTAYVYVSKAAAYYAQASLGLSPQRMLIIPNGIDLEKLDAARAATDRAAQRLALGL
                     AEQDFVFLNVASIYPPKAQGLLVAALHRARQHNPHLKLALLGPIMDEAYAERVREQAE
                     RLGVADAILWLGHRPDPQQLYMLCDAFVLPSFVEGWSLSLAEALYARLPIAATDVGAA
                     AELVDPQRGRLVPPPYASILDIDGAESFARLLAKEQPQFVENLSAAMLAVSECAPPPP
                     IPPDLARRLDRRHAYQGYVRLFESLAAGEHAVVRSCVSNLHEADPSQCAALDAQPAAG
                     PGARKESQNQCSEMIAK"
     gene            complement(25665..26558)
                     /locus_tag="PLU99_01610"
     CDS             complement(25665..26558)
                     /locus_tag="PLU99_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15780.1"
                     /translation="MSGEEVSVAQVQTYVHQSRSVAYYLRPDLIPRTLWRHRQLIGQL
                     TKRNLLLRYRGSALGLLWSLLMPLLMLVVYTFAFGFVLQARWGLGDESKVRFALKLFC
                     GLLLFNIFAETASACAALIVAHANYVKKVVFPLEILPAVALLTALVQGLISSLVLLTG
                     IVLLEQRLPPALAYYPLTFIPLLLLCLGLGWFLASIGVYVRDVGQAITVIIQILIFMT
                     PIFYPLDMPALQAHPNVQFVMRLNPLTVVVENARVTLMGTPGQSLEWLWLGIVTALSL
                     IVMQLGYAWFMKTKRGFADVI"
BASE COUNT         4845 a         8652 c         8619 g         4716 t
ORIGIN      
        1 gccaggatcg ggacatgctg ctcgccgagg tcgcggccac ctcgacggcc gtggtgggcg
       61 agcacttcgt cgggttggcg attgacgaag acacggaaaa gcatctgacg gcgcagcgcg
      121 tgcgcgaatg ggtcgagcag attcggccgc actttgcctg aacgcaccac gatgacaacg
      181 aatgccgcct cgggggttgg ttaaggttcg tcaggctggg tcgttctcgc tgccgggcat
      241 actgcagggt ccaatgtgcg gcttattttt cccgccgggc aagccgcggg gtccgctgtg
      301 ccggccatct gcccctccgc gggcccgttc gattttaagt cgcggctgat gcgcagactg
      361 caccagtcgc ggccgcacat cgaacagtag tcggtgtcag tctgaatatc ctcatcgtgc
      421 agcgcccggg ccgtctcgcc gtcgaaggcc agctcgaact gccgcgccca atccagcgcc
      481 gcacgggccc ggctcatatc gtcgtcccac tgccgggcgc cacggatacc gcgcgcgacg
      541 tcaccggcgt gggccgcgat tttgtaagcg atgcagccgg cctttacgtc cgcggccttg
      601 ggcaggccga cgtgctccct gggagtgaca taacacagca gggccgcacc ggctcttgcc
      661 gcttcggtcg cgccgatcgc gctggtgatg tgatcgtagc cggggaaaac atcgctcacg
      721 accggcccga gcacgtagaa cggagcgtcg tcgcagacgg cgcgctgctt ctccatgttc
      781 cacgccacct cgttcatggg aacgtgcccg ggaccttcaa ccatcacctg cacgccggcc
      841 gcacgggccc gctgcaccag ctcgcccagt acgtgcaact cggcgatttg agccggatcg
      901 ctggcgtcgg ccaaacatcc gggccgcacg ccgtcgccca gcgaataggc gacgtcgtgc
      961 tcgcgcatga tggcgctgat ctcgtcgaac agctcgtaaa aaggattctg ccggccgtga
     1021 tacgtcatcc agcgggcgat gagcgagccg ccgcggctga ccagcccggt cgtacggcgg
     1081 cgaatcaggc ccaggtgctc gcgcagtatg ccggcgtgaa tggtgaagaa atccacgcct
     1141 tgctcggctt ggcgggtaac cgttcgcagg atcgtgtcgt aatccagttc ttcgatcgtc
     1201 cgtccgacga tcatgctgta aatcggcacc gtgccaatgg gaatcgtgct gttgtcgatg
     1261 atcgcctggc ggcagcaatc cagctcgccg cccgtcgaca ggtccatcag cgcgtcggcc
     1321 ccgtagagtt gcgcccaccg cagcttctca acctccgagg ccgtatcgct ggccaccggt
     1381 gacgccccga tgttcgcgtt gattttcgtg gagatggcgc gccccacgcc catcgggtcc
     1441 aggcgcttcg gcgcccgttc gccgcggcag tactccgggt cagccagcag ttgccgccgc
     1501 tgcgcaaccg tctggttaac ccaaagcatg ctctcgggcg cacatccggg atgtccgacc
     1561 ggctgttgct ccgcggaggc gggccgagtg gcggcagaat ccgccgccgg cgcctcccct
     1621 ccgctacccg ccaggtgccg gaggttggcc ggaatgatga tccttcccgt cgccacctgg
     1681 tcgcgaatga actcaggcgt aacgctctcg cggcacgcga cgcgccgcat ttcgggagta
     1741 attacgcctt ttcgggccgc ctcgagctga gtcatgccta tctccaaggc ccaccatggg
     1801 ccgggtcgta cagaatacca cgccgtaaga gcgggtgcat tgggccatgg ctccatgcgt
     1861 tgcgcaggtg ggtggcgctg tgtggaaaca agcgggcatg cgccggttgc gcgggagtgc
     1921 gttctgcctt cggtgcatcg ccaaaagcgg ctgaatcgca gccgccgccg ttcacggttg
     1981 gttcacacct tccgatgcgc cgtccgcgcc acctgcgtcc catccgccag ccgcaccacc
     2041 tcgtagtccc cgaacaagtg cggcgcttcc tgctgcagca ggcggagaat ttgcagcgcc
     2101 accttgcgga tttcgacctc ggcgtgctca ttggcccgca tctcgatgaa atgccgccag
     2161 gcccggccgt tcccggtcac gaaaatcttt gtctcggttg cgttcggcag aacgctgcgc
     2221 gcggcttgcc gggccagctt ccgccgcatc gtcgcggagg gctcgttcgg aaacttcccc
     2281 gccaacccct cgaccagctt gcagtacgcc gcgtgcgcct gctggatcga ctccagccac
     2341 accttgtgga gctccggatc ttccgcgatc acatcgggtt ccacgaaatc cgcggtgctt
     2401 tcgtcaacgt accgttgcga aagctgcgaa tacccgaacc cggcccggtg ccgcaccagc
     2461 tcatgcgtga acgaccgcga aacgccggta atcagcaggt tccaagccac gtgctccagc
     2521 accgacccgt gctgcgactc gatgatgttc gccaaatact cggcattcgt cttccgcccc
     2581 tggccgaaag acatgtagca caggcgcccg gccacctcac atagcgcttc ggccccgacc
     2641 tcggtgtccg tgctccaact gagcccgtgt tccttcagaa agcgctcaat ctcggccgaa
     2701 ttcgccgcca cccggcccac aatgtacacc cgcggctcgc gaataatctg catcaaccac
     2761 ctcgcaacgc acgccccaac cagcgggcat tctagcgggc tggggcgccc tgcgcaagcc
     2821 ggggctgagt gccatgccca agccgcgcgg cggcgcggac atgccctcgc cagaagaagt
     2881 aggcgctccg ccccgaagag cgcggactgg ttggtattct ccggggcgac acgccccggc
     2941 tctgctcttt agcgccgctc ccacacgccc cagctctgct tatttcgctc tatcccggtc
     3001 ggcgcaatgg caagcattct tagttcgcgt cgcccggata cgttagcact ccgcgcacga
     3061 tccgggcggc cgacgtgtac gggtgctcct gggttttgcc ggctgaaaga atgtgccaga
     3121 cttggatgcc gctcaccttc aggtaatccg agacaagcgc gcggtgacaa cgccaccaca
     3181 gcgcctcggc acacatgatg actgttgggt gcgcggccgc aagcgcgagc aggcgttcaa
     3241 tgcccgcgtg gaattccggt gtctgcatgt agtcggcgta accgcgaaag gaggcgttgc
     3301 gccaggccgc gttcacagat tggcggctgg ggcgccggcg tccgccgagt tcgcgcaggg
     3361 actcgtactc aatatccgca gcgcgcagcg tggccgacag cggccctgag ttgaaccacg
     3421 gatacttgcg cgacccggga aaactgcgga tgtcggctac ggtgtgaatc gcgtgagccg
     3481 tcagcatgtc gataaacacg ctcagcggat gattggaatg ccctatagtc cagatgtgca
     3541 tccggtcgcg attcggcgcg ggccggccgc tcgctcgccg ggcggccgcc ggctgctttg
     3601 atcatacgcg atccacgcgc aggccccgcc tgagtagggc gatcctgcct ggcggatgaa
     3661 aagtggcgat ttgatttcgc ccctgttatc gtttccgtat gggattattc gacgtattca
     3721 aaaagggtct ccaaaagacc cgcgagaaga tatccgccgg actgcgcacc atcctgccgt
     3781 tcggccgcaa gatcgacgaa cagcttttgg acgatctcgc cgacgccatg cttacgggcg
     3841 acatgggtcc gcgggccgtc tccgggttga ttggcgaggt tcgcgcggcg tggaaggccg
     3901 ggcaactcac cgaggcgcag gagatcctcc cgttcctcaa acgccgcatc gccgaaacct
     3961 ggaaaccggg cgaccgcgcc ttgcggttgg cccccagccc gccgacggtt gtcttcgtcg
     4021 tgggcgtgaa cggctcgggc aagactacca gcgtcgccaa gctggccgcc tggctgcgca
     4081 aacagggcaa aacggtcctg ctcggcgcct gcgatacttt ccgcgcggcc gccatcgatc
     4141 agctcgtcat ctgggccaag cgcgcgggtg tggacatcgt caagcatcag cccaacagcg
     4201 accccggcgc cgtggccttt gacgcctgcg aagcggccct ggcccgcaag gtggacgtac
     4261 tgctgatcga taccgccggc cgcctgcaca cgcaggatca cctgatgcgt gagttgggca
     4321 agatcaagcg tgtggtgcag aagcgcattc ccgacgcgcc gcacgaagtg atcctggtgc
     4381 tggatgcgac cattgggcag aacgccatca atcaggccaa ggtgttctct gaggtgacca
     4441 acgtgacggg actgtttctg gccaagctgg acggctcggc gaaaggtggg attgtggtcg
     4501 gcatccgcga ccagcttgac atcccggtca agttcgtggg cctcggtgaa acgatcgatg
     4561 cgatcgagcc tttcgacccc gaaactttcg cggaggcact gttcgcggaa gcctagttag
     4621 cagttaaccg cgccgattgc ggcgttaatg caggcggggc gagtggcgca cggggcggga
     4681 gatccttggc gcaatccggg gcgacacgcc ccggctctgc ttttgaatta atcgagattc
     4741 acgctctcct catggcgatt tcagtccagc gacgtaactg attcaaattg gccagaggtt
     4801 tttcggtcga aggtctgtct cggccgcttc tggcgtcgaa cgccgtgaaa aggatctcga
     4861 tatgattgaa agcagatacc ccgtaccgga aggccaggcc gtacgtccgg ccgaacctgg
     4921 ggtccagcct gaaacacgcc cccgcgagtt ccagcccgaa acccgccccc gcgagttcaa
     4981 gaccactgcg ggcgtcgtgg ccggcggttc ggccatcgag acgctggccg gagcggcagc
     5041 cgtggttctg accattattg ggttggccgg ctggtacccg gctctgctct tgcccatcgc
     5101 cgccatctgc gtcggggccg gtctcatgct taagggcgga gcgctggccg cgcgttcgca
     5161 tttcctgatg cgcgaggtct cgggcgaaag catgaccatg gcagaactgg gcggcggaat
     5221 gtcggccgaa ctcatgggtg gggcggccgg catcgtgcta ggcattctgg cactggccgg
     5281 catcgcgcct tctacgttgc tgtcggttgc ggccatcgta ttcggcggca cgttgatgtt
     5341 cggcactggg gctgaggcgc ccctggcgcg tctgaccgcg catccggcgg tgcaccctgc
     5401 ggtgcatgcc gcagcacggg acatggccca tgcttcggca tcgagccagt tcctggtcgg
     5461 cgccggggca gtcgtgctcg gaattctggc cctgatcggg ttcgtgcccc agacgctgca
     5521 gcttgttgcg atcctggccg tcggtggcgc ggccttcctg gccggcgcag tcgccagtgg
     5581 gcgcatggcg tccgtgtttc gcagcgcgta gctcctccga agagaggcga gcggctgcgg
     5641 tacgtgcttg ttccatgaca gcattggccg gtagcggccg ggccaaatcc catgtggccc
     5701 ggttgcgacc ggtctgcgcg tgggtcgggc aatttctccc gggcgaggcg ggctagcgcg
     5761 tgcggtcgac tagcgctgca gtttttctat cggggccatt tcgaggataa acgttttctc
     5821 gccgtggcgc gcgaaacgga tcgcagcccg cgtctgaccg ctgcccgggc gaatccagac
     5881 tatctgcccg acgccgtaac gggcgtggcg caccaacgtg ccgggtttcc aatcggcgta
     5941 gacggaagtg tcttgcagcg cctcgccggc ggcattgatg atgcgcacgt catcttcgtc
     6001 gccaaactcc gatgatctgc gccgttggtg attccacttt gagcccccgc ccgcgcgcgg
     6061 gcgaagcggg cgctgccctt cttccggcgg gagatggtcg tgcagggcta caaaaccgtc
     6121 cggatcggct tgctcttcct ccgcctgcgc ccggcccccg ccggaatccc agggctcgcg
     6181 gctgtagtcc tcaaaccggg cggcgctctg gtccagctcg cgtaagaatt gggacgcggc
     6241 ccgcggcagc gtgacgccgc ggatcatgcg ctggcgggcg tgcgtgagaa acagccgctg
     6301 gcgggcgcgc gtaatgccca cgaagcacag ccggcgttcc tcctcgacgt ccaagctctg
     6361 catcaaggcg ttttcatgcg gcagcaggcc ctgctccaga ccgacgatga acacgaccgg
     6421 gaattcgagc cccttggccg cgtgcagcgt catcagcatg accgctcccg ccttctcgtc
     6481 cacctggtcc tggtcgctga ccagcgcgat acggtaaagg aaatccgcca gtgtcggctc
     6541 ttcgacttcc tcctcgtaac gccgcgcggc ggtgaccagt tcctgcacgt tggcgaggcg
     6601 gtcttcgccg ccctcctccg cctctttcgt tagcgcctcc tccaggccgg atagcgacag
     6661 cacggcgctg accactccgg aaaccgggcc ccgcgccgcc tgctcaatct gatccagcag
     6721 ctttacgaac gccgccaatt tcttgcctgc acccttcatt tccgcctgaa cgccgcggtc
     6781 gcgcaggacc tcgagtagcg gccggtgctg gcgctcggcg gttgccagca ggcgctcgat
     6841 ggtggtcttg ccgatgccgc gcgccggcgt attgatgatc cgcagcaagg ccacctggtc
     6901 ggccgggtta attaggatgc gcaggtagcc gagcgtgtcc ttgatctcct tgcgattgta
     6961 gaactcgacc ccgcgcgcga tgcgataggg aatgccgcgg atgcgcagcg attcttccaa
     7021 gccgcgcgag atggcgttca cgcggtagaa aatggccacg tcgctatatg cctgtccgcc
     7081 ctggtgcagg cgctgaatcg tgtcggcgat ctgctcggcc tcgtcctggc cttcgctgaa
     7141 gcgccacacg taaaccggct cgccgggctg attctcggtc catagatcct tgtgcttgcg
     7201 gcggcggttg ttggcgataa tggccgaagc ggcctggagg atcatgcccg tcgagcggta
     7261 gttctgctcc aggcgtacga cgactgcgtc cggatagtcg cgttcaaatt cgagaatgtt
     7321 tttgatgtcc gcgccgcgcc agccgtagat gctctggtcg gggtcgccgg tcacgcaaat
     7381 gttgcggtgc cgctcggaaa ggtaacgcgc tatcaggtac tgcgcgtggt tcgtgtcctg
     7441 gtattcgtcc accagcacgt gctgaaagcg aatgttgagc cgttcaacga tatccgcgtg
     7501 gccgcgcaac agcagggcca cgcgcatcag caagtcgtcg aaatcgacgc cgttgcgctc
     7561 gcgcagcagc tcttcatact tctcgtacac gcgtgccacg gcacgctgct cgaacgtatc
     7621 cgcccagccc gcgaactccg ccggcgactg gagcttgttc ttggcgttgc tgatctcgtc
     7681 ctggaccgcc tcgggccgca gcaggacgtc gcgcactccg caggcctgca tggcctcttt
     7741 tacgacgcgc agccggtcgg cctcgtcgta gatggaaaac ccgggtaaga cgccgaccaa
     7801 ctcgccgaac tcgcgcagca gccgtacgcc gagggcgtgg aacgtgtgca cccacatgcc
     7861 ccgcgcgacg cccagaccct cgatgcgccg cttcatctcg tcggcggcct tgttggtgaa
     7921 cgtaatggcc aggatgttcc gggccggcac accggtgcct accaggtaag ccgcccggcg
     7981 ggtgatgacg cgggtcttgc cgctgccagg cccggccagg accagcaacg gcccggcgcc
     8041 gtgcgtgacg gcctgacgct ggggctcgtt caggtcggcg agtatggcgg atggctcaag
     8101 gtgagcgtgc atggtctcgt atatgttact tcgccgcggg cggtttggca gcgcggccgg
     8161 gaggacgctg agggagcgga tcgaacatgg agagcctggg cacggctttc cgcgtggcgc
     8221 attttcggtc ccaaggctga ggcctggacg tggcggcgcg gctggctacg gccggcgacg
     8281 gggccgttgg gctggttctg ggaccagccg gtgtaaaaac gcgatttgca gggcttcgag
     8341 gcgcctcagc gtcgaaatca aaggaggtcg atcggcgggc tgcctagtat tgaacggcta
     8401 cagcgaactc cagacctgtt gtctctctcc tttgcacgca gcagtactga tgggccggtc
     8461 gcacgaagcg gccggcccac tgcgttccct cagctccggt ttcgcgcttc agttttccag
     8521 gccttgaaac ttgaaacccg aaactggaaa ctacccgcgg tcgtggtctg agaaaattgg
     8581 agcatgggta tgaagcgttc agcgctaagc gctgcaccaa tggtgcggct gggcctgtta
     8641 ttgtcctgcg cggtcctcct tgacgggtgt gccagccagc gcgccgtctc caccgaagac
     8701 gctctggtgc ggcaggcggc cgaggcctac gcggcccgct gcaaaacggg catggccgct
     8761 gatacgacag cgtcgccctc tgagattctc agttatgagt ctcagatgcc atggccttct
     8821 gcgcctggtg ggagcgcgcc cggcgcggcg tcttctagct ctgccgcatg gccgatcgcg
     8881 ctgggagcag caaattcgct cagccacgat gatgcagcaa ggggtaggtt tgcggcgggt
     8941 ccaatattta cattgggcag cttcgatctg ggccaggacc ctgagcccga aatgggagca
     9001 gcaaccgcca gcgccccatg tgtgccggaa ggctactggc gcgcggacat ctggcaccag
     9061 atgggccacg aggcgctgga attggccacg cacggctttt ggcgcggctt caaggtcagt
     9121 ttctgggacg tcgaaaacgc gcttgttttg acggctgcca tgggggccag catcacaatc
     9181 cgcgagaccg gcgtggacga tgctatccgt cgccggacga agggcgaaaa cgagttgggc
     9241 agcatggacg agacgatcca gattctcgga aatcccggga cgcatttcgc cggcgcgggt
     9301 gtgctgtggc tggccagcgc tttgacgaaa gacgtgcgcg agcacgaggt cgccaagtcg
     9361 ctggtggagg cgctggcggt caacggcgta tcgaccatcc tgctgaaggt ggcgacgaac
     9421 acgcgggcgc cggatgggga ggactacgcc tggccaagcg gtcacgcctc gagttctttt
     9481 tgcaccgcgg cggtgctgaa cgagtactac ggcccgtggg tgggcatacc gtcgtttgcg
     9541 ctggccggct tggtgggcta tcaacgcctc gactcgcgcg tgcacgattt ctcagacgtg
     9601 gtcttcggca gcttcatggg ttacgtaatc ggctcctcca ttgccaaaga caacaagatg
     9661 gaactgccgg agttatttgg aatgaaagta ataccgttcg ctgatccgca aaccggcgcc
     9721 tctgggttgg cattattcaa acaatggtaa gaagcgccag cgggtagact tacgacatcc
     9781 cggatttctg tagctggaat tcgccgctag ttgtcattgc agtttgcgtt ctcgctaagc
     9841 cgcgaagcct atagcgtgca aaagctgtgg ataactggtg ttcttaaaaa tttttcatcg
     9901 caattttttg aggtttttgc gcaactcgaa tccattcact cggccgcgcc agccgcgttt
     9961 caggagccaa atactgcatg ttgacaggct gtcaataagt cgttcggccg tttttacatt
    10021 gactaaacag gcattttgag ctatccttaa agttgcgacg ttgaggtcgc gaactcggca
    10081 cctcagccgg tcctgtccaa ctccggtaga gtgtgcgcgt cacccaaaga tcgctgttta
    10141 tcgggcagta atgtccgggg cagcgaaagg tgaaccaaat agcccgggtg acacctccgc
    10201 tcgaatgtac aggatcgcgc cggtgcggaa accctatgag ggttgcccgt ttaatctact
    10261 gcggcttgcc gcagcaggtt tccgggccag acggacgccg attgagactc tgaggcgccg
    10321 ctaacgcgct tccgggcgcg tgtagaaaag cgcgtgagag gagaacgggc cggaacccgt
    10381 tccagtcgtc cgtcgcaaac cctggaaggg caaaacccaa gggagcatcc agcggtctca
    10441 gcaccctaac caaggtgctg gaacgccagg gattctcgcg agggtcaaag cccaggaacc
    10501 gcggcctgcc gggccggccc gtcgctccgg cgacggaagc aacggcagga aaaacggtaa
    10561 gtgggttcac cgagccggaa acgacccggt accttgcggg aggagtaagc tccgaagggt
    10621 gaatcccaag agcgccgccg gtacgaaaga agaccggccc gggctcggaa ggagtaagcc
    10681 gcgcagaggg taaccaaacc ctgaacgcgg aagttgacgg gcgtggcaag cccgtgtgaa
    10741 ccggacttcc gagccttgta agtgctgtag ggagcaaaag cccatgagag gagccggctg
    10801 gctgcggccg tcggctaggg tccgctgagg tattctcagg cggagagcga aactcgaaga
    10861 gaggatttgt gccggttact taactgtgac cagccgagtc cggcgaaagg aaaaccgcac
    10921 agccgaggaa atggccaacg gcatagcggg accgccaaac caataacggc ggtacttctc
    10981 cggacaaaaa ctctgaacga gcagctaact ccgtgaggac gcgacccggt tcgtccgggt
    11041 ttggcgatca gcggaaccga atactctgaa gggcccacga gttcgcccga ccttagcgcc
    11101 gcattttttt ttgcgcgcga acagcccgtc gccccgcttt cggcgcgtct ttctgcagag
    11161 cgagggatac gggcaagcga cggggacaca cggcacgcga tcaggggcac acggcggtat
    11221 accgggccgg cacggcccgg ctctgctctg cggggcgcgc cgcatcgccg tcatacggcg
    11281 gcgtgaaccg ccgctattac ccgctcgatc tgctctgccg tcagctccgc gtacatcggt
    11341 aaactcagtt ccgtcgcgcc gatctcctcc gaaacgggca tgctgcccgc tcggtagccg
    11401 aacggctcga acggcttttg caggtgaatc ggcgtggggt agtgcaggtt ggtgccgatg
    11461 ccgcattcct gaagattggc cttcagctca tcccttttct cgcagcgcag cgcgtaaatg
    11521 tggtagacgt gctcgccatc ggggcaaggc ggcggagtga ttacgccggc gctggccagc
    11581 ttgcggtcat actcagccgc gacgcggcgg cgggcggcgt tccagcgatc cagatatttc
    11641 agcttcaccc ccagcaccgc gccttggaag ccgtccatgc ggtagttgaa gcccagccgt
    11701 tcgtgaacgt agcgcgtctt ctgtccgtga tcacgcaact gccgcgccag gtcggccagc
    11761 tccgcgtcgt gcgtgaccag cgcgccgcct tcgccgtatg cgccgaggtt cttgccggga
    11821 taaaagctga agcacgccag gcgtcccagc gcgccggcgc ggcggccttt gtacgtcgcg
    11881 ccgtgagcct gggccgcatc ttcgatgacc ggcacgccgc gctcggccgc gatggcgagg
    11941 attgggtcca tatcggcgca ctggccgtac aggtgtaccg ggatgatggc cttggttttc
    12001 ttcgtgatgg cccgcgcaag ctggttcggg tcgagcgtgt agcgccgcgg atcgacgtcc
    12061 acgaatacgg gccgcgcccc gacatacagc acgggccagg cggtggcgac gaagctcatg
    12121 gccggcacga tgacttcatc gcccgggccg atgtccaggc acagcagcgc gacgtgcagg
    12181 gcgctggtgc cgctgttcag agccacgcag tgctcgaccc cgcagtattc ggcgaatgcc
    12241 ttctcaaacc gctgcacggc cgggccaagc acgaaggcgg tgttggccag cacctcgtcg
    12301 agggcggcgc gaatctcgcc ggcgatagtg gcgtactgcg ctttcagatc gagaaagggc
    12361 acggacactg tcattgttcg gctccagaag aaagatacgg cagtctagct ggtttacggc
    12421 aaccctccca cccgtgctgt acctttgtag cgcgcttgaa cgcgctcgta gaaggcctcc
    12481 atttgcgcgg cgatgctttc ccagctcaga tgctcgagca cgaaccgtcg cccggccgcg
    12541 ccgattcgtt gccgcagctc cgcgtcgcgc agcaacttca ccagcgcgcc ggcgatgctg
    12601 tgtgggtcgt gctgatttac gagaattatg ttctcgccat tgcgcagccg gccttcgccg
    12661 aacaggttct ccggcaggtc attcacgacg ggcgtttcgc agagcatggc ttccatcggg
    12721 gcggtgccca ggccgggaaa tggtccggtg gcataactga cgaagacatg cgcctgttgc
    12781 atgagccgca cgacctggtc gtgggccgtc tcgcccagga actgcacgtg ctgactcaaa
    12841 ccgagggcgt gcgcgacctc gggcggctct ttcagatcaa cgcgtccggc cagcaccaag
    12901 cgggcttgcg gcaccgcttg caggaccagc ggcatggcgc ggatgatctg gaccggattg
    12961 cggaacgggt gaatttgccc cacgaacagg atcaacggct cagccgcgcg ggccacagtg
    13021 cgattctgat attcggccag cgcttcgcgc cgcacgccga agagaatgat ctccgagcgt
    13081 tgctgcgcgc gccggccgaa acggacgccg acatagtgat gcaccatctg gtcgagactg
    13141 acgatgccat cccagcgccg cacgccgccc cagccgagcg ccacgcgatc gcccagggcc
    13201 atcaccaggt tgcgcaacgg cttttcgtgc tgaaccggcg ttgtcagcga gcccaccagc
    13261 ggaacgcgcg cggcgcgggc cgcgctggcg gtcagaagca cggaatcgaa gatgtgatta
    13321 acgtgatgca gcacgtgcgg gcgaaagcgc gcgatgattt gcagcaagcg cttgcgatta
    13381 gccggcgtga aggtgaaccc catcagccgg gcattgtaga agagcgccgt ccgcggcaaa
    13441 cgcaggcacg gcagacggtg tacggggaac ggtgccgcag gcgcgtactc gggaccccag
    13501 tcgaccgtca ctacctccac ctcgtgcccg cgttcgacca gcgcctgggc caggctgctg
    13561 gtgtagaacg acgacccgga gatcaccggc gggaaccagt tgctgaagaa caagacccgc
    13621 atcgctggtc ggctccgggc gaattcaact caccgccgca ctcagggccg cacaaacgcg
    13681 atcctggtcg gcttcggtca tatggtgata caacggcagc agaatggccg tgtcctgggc
    13741 tagctcgctc tcggccaatc gccggcagcg cccgggttcg cagccgcatg ggccgggccc
    13801 ggcgccgcag ctccatgctt cctctcggta tgcgggctca cgatgggcgc acatgattcc
    13861 gcgccgcgtg gccactctcg cgtccagcat gcgctgcatt acgcggcgct ggtcgcaacc
    13921 ggccggcagt cgcacgcaat agctctgcca gttgctgcgg gcccactccg gttcgacggg
    13981 cagccgcagg ccgggcacgc cggaaagccg ctcgacatac ccggcggcga gctcacgccg
    14041 ccgggcgatg atcgcgggta ggcgcttgag ctgctcgcgc ccgaccgcgg cctgtatgtc
    14101 ggtcatgcgg tagttgtagc ctagcgtcgg gtaagactcg aagatgacct ggttggcgcc
    14161 gtgccgaaca gtgtcgggca cgctcatggc gtgttggcgc caaagccgga actgggcatc
    14221 aagcttcgca tccgcggtcg tcagcatgcc gccgtcgccg gtggtaatga ccttgcgcgg
    14281 gtggaacgaa aagcacgcga tcagcccatg cggcttgccg atccgctccc agcggccttc
    14341 ccagaggatt tcgctgccgg aggcgcacgc ggcatcttca atcagcggca ggttgtggcg
    14401 ccgggcgatc gtgagcagcg cggccagatc acacggcatg cccatttgat gtacacagag
    14461 gatggcgcgt gtgcgcggcg tgattgcttg ctcgaccagc gccggcgaca tattgaacgt
    14521 gccgggctca atgtctacga ataccggcgt agcgcccgtg taccgaatgc tattggcggt
    14581 cgcgatgtac gaatggctga cggtgattac ctcatcgccg gcacgcacgc cggccgctcg
    14641 caaggccagg tgcagcgcga ccgtgcagtt cgcgacggcg caggcgtgcg gcgcaccgac
    14701 cgccgcggcg aattcccgtt caaaggcggc cacttctggc ccctgcgtta cccagccgga
    14761 gagaatcggc cggcgcacgg cggcggcttc gccctcgtcc atccaaggct tggcgaccgg
    14821 aatcagcggc agatcactgc tcatgttcag ggctttccaa gtttgcggta ctccggcggc
    14881 agctcaacgc gcggccggtc ggccggcacg aaccgcgccc ggtaagactt caggaacgag
    14941 atagtgctga tctcggctgg cgttcggcag cgcgtgtact gccggcgcgt caggtcgaaa
    15001 accgtgacca agtccggcgg ggcctctgct tcgttgcgac gcgtaaacac cagcagggga
    15061 atgtccgacg ccagacacgc ggccgaatcc acgtccggct gccatcgcag gcggaatggc
    15121 ccgaagggcg cgcggacgac atagccgttt ggctcagtct caaacgcggc cggaaagacg
    15181 gtctgcacgt attccgccag gaactcaccg atgcgcgcga gcttctcgtc cggcgtaacg
    15241 tggcgatacg gcaccagccg atcagtcata tcccgggccg gcgtgccgcc gaagcaatga
    15301 ttcggcggaa cgtctttggt cacacacgaa ccggccaaca cgagcgcctt ctcacccact
    15361 cttacgcccg gcgcgacgct ggtgtaagag cccatgatcc aggcgtcgtc ttcgatcgtg
    15421 accgggacta ccttgaaaac ctggcagcct tcgagcagat cgccgaagta gccgtgcgaa
    15481 tacagattcg tgttcgtgcc gacgcagacg ctattgccga tggacagact ggcttcagaa
    15541 ttgagtatgc aatgctgtcc gatccagcaa ttgtggccaa tccgcagcgg gccgcggccg
    15601 ttcatcagcg tatggttgtg aattgcgaca tagtcgccca gcacggcggc cggacagagc
    15661 agcttgcagt cgtgcgcgat cagcgattgt tcaccaatat cgatgaagtc ggccggaccg
    15721 cgcattccgc cgactacaca gcgcgcttcc agccgcgcgc cgtgccgcag caccagccgg
    15781 cgacattcaa tacgcacgtc atcaccgata cgcacgtcgt cgccgagttg cacgtgctcg
    15841 gcggaaatgt gcacgttgcg gccgacgcgc acgctcggat tgataccggc cagatccatt
    15901 acttcctctg ggtcgaacag ggcgtctgcg ccgggtcgta ttcgcgcatg tgagccggtg
    15961 tgatctcacg gcgccaggcc accagccgcc gcagtccctc ttccagcccg acttgcgcct
    16021 ggaagccgag cagccggcgg gctttttcgg tcgaggccag gcggcgcggg acggggttga
    16081 ccttgcgctc aggcaggaat tcgggttgca cgtcggtccg gccggtcacc ttgagcagcg
    16141 tttcgagcaa gccgcgcagg ctcgtctcct gccccgacgc gacgttgaac acctcgtcgc
    16201 tgacgtcgct cttggcggcc agcacgttgg cccgcgccac gtcttcactg aagatgaagt
    16261 ccatggtctg gtggccatcg ccgaagattt tgggcggaat accgttgtcg aggcagtcca
    16321 gccagcggat gagcacttcg gtgtacttgc cgaaaacgtc catgcgcggg ccgtagacgt
    16381 tgaaatagcg cagtgcgacg tacggcaggc cggtcatgtc gttgaacgcg cgatacaggg
    16441 cttcgttggc gatcttggcc gccccgtacc aggtgcggtt gttgtacaag tggtgccgct
    16501 catccgttgg gaaggtgtcg gccaggccgt agaccgaggc ggaactggcc gcgaccaggc
    16561 gctgcacgcc ggcctgcacg cacgcctcca cgacgttgaa tgtgccggtg accagcacgt
    16621 cgaggcactc gcgccactgc tcggcgcagg cggtgatacg aatggcggcc tgatggaata
    16681 ccacgtcaac gccctcgaag tgcgggcgta tgtgggcgaa gttgcgaata tcctgccgga
    16741 taagctgcac atcgccatgg gcctgggccc actggatatt caaaagcgtg ccgcgcgtca
    16801 ggttgtcgat aatgcgcacg gtgcggacgc cttcgtcgcg cactaattgg tcgatgatgg
    16861 ttgagccgat caggccgcag ccaccggtca caagtacggt ctttcctctc aggtccattc
    16921 gcgcctgcct gttattccaa atccggggcc cgcggccgtt gcagccgcgc gggtacgccg
    16981 gctaccaccg ctccgggcgg tacgtcgtgc gtgactactg cgccggcgcc gacgatcgcc
    17041 tgggctccga ttttgactcc gcacatgata accgcgccgg acccgagcga agcgccgcga
    17101 ccgacgtgcg tcggcacgac tttccaatcc gcctcggatt gcggccgccc gccgggcgtg
    17161 gtggcgcgcg ggtaacggtc attgatgaac agcacgccgt gaccgacgaa aacctcgtct
    17221 tctatgtgta cgccttcgca gatgaaggta tggctggata tcttgcagcg ccggccgatc
    17281 gtggcgtttt tctggatttc cacgaaggcc ccgacgcggc tctcatcacc gatggtgcag
    17341 ccgtaaagat tgacgaagca gtggattttg acgtcgcgtc cgaggacgac gtccttggcg
    17401 atccggcaga attccacgat tcctcctctg gtgttcgctg tagccggcga gccttacatc
    17461 aacagcaccg ggctgccctc gcgttgcagg ctgcgctcgg cggcggtcag cagcttgacg
    17521 acacgcaagc cggcctggcc gtcggtcagc ggcgcgcggt gttcgcgaat tgcatccagg
    17581 aattcgcggc acatgagact cagcggctcg acgccgggga tttgaggcgc gtacatgtcg
    17641 ccgatgcggt actgcacgag cgtcttgtgc acgccttcgc gctcgatgaa gtccacacct
    17701 ttgtcgtaaa ccttgacctg ctcttcgggc aaggccatgt cgtagacgac catcttttgc
    17761 gtaccgccca caatgatgcg gcggaccttg gtgggcgaca tccaattcag atgaaagtgc
    17821 gcgagcgtct gatccgcgaa cttggcgacg atgaacgcca cgctggcttg ctcatgcccg
    17881 aaagccgagc ccaaagcggt catgctggtc ggctgtttgc cgacgagata gtccatgatc
    17941 gcaaaatcgt gcgggccaag gtcccagacg acgctgacgt cgtgctggaa caagcccagg
    18001 ttgatgcgcg tcgaatcgta gtacagcacg tcgcccagtc ggccggagcc gatcagctcg
    18061 cgagtggtgc ggatggccgg gttgtagaga aaggtgtgat cgaccattag ccgcaggtcg
    18121 cgctttgccg ccaggcgcac cagttcctcg gcttctgcga cggtggccgt catgggtttt
    18181 tcgagcagca cgtgcttgcc gcactccagg gcccgcttgg cgagggcgaa gtgcgtcgag
    18241 acgggcgtgg cgatgactac ggcgtcgatt gactcattgc tcagtaacag gtctgcgtct
    18301 gtggcagtct ccagggcggg gtacatggcc ttggctttcg cgagccgcgc cggctgtggg
    18361 tcggcgagca taaccggcat agcccccggt gacccggcga agttacgcag cagattcgga
    18421 ccccagtagc cgtaacccat caaacccagg cggatcgtct gctgcttgtc ggtactctgg
    18481 ctcatgcttc ctccgccgcc aacagggctg ttgtgctttg gcgatgttgc ggttgcgact
    18541 gttcgggggc cgtcagctca agcgcggccg cgccaaacgt gaggccgtcc acaggcgctg
    18601 aggccctggc caacacgtcc aattccggct tgcgccacag agacaaccgc ccgcctgcga
    18661 tggtccagca aatcgcgccg attaccaggg cgcacacaag atcgagggca tgataggcta
    18721 tgatgcacgc gcccgccaaa ccgggtgcga cgccgaccaa acccaacgcc accagtccaa
    18781 aatagtgcac cgtgccgata tttcccggcg cggccggcag gatcgtgctg gccgcgatca
    18841 agcccatgat gagcggggcg tagccggcgg ggagttcaat tgagaaagcc cgcagcgcta
    18901 tgagcaacga cagcgaaaga acaccccaca tcagagccgt gcttgcgagg aaggctaaca
    18961 ggagcggcgc gcggccggcg gtggttaatc ctcgtccgaa tttgcgcatt acttccagcg
    19021 ccccggttcc caggcgccgc tgcagaatgt ccgcggcgta gttgaggcaa cgtactcccc
    19081 actgtcgccg cagcacgagt ggcagcagca aggcgaggct tgccgtcgca accgcgatca
    19141 agctggcgcc gcgcaaggcg cgcagcgcgg gactgggcag gaaccaggtc gcgcccagca
    19201 ccagtgcaaa cagaatcacc gcatcggtga ggcgcgttac gaccatgatg ccgagcgatt
    19261 gaatgcggct gaggtccgcc agtcgcccca aagcatgggc cccgaccgct tcgcccagct
    19321 tgcctggcag gattgcgtta gctccctgtc cgatgccggc cgccagcgcc agcgggccaa
    19381 tccgtgcggc accctgctgc gccagcacca cccgccaacg caaggcacgc cacaagactg
    19441 acgagcacaa cagcggcacg cacaacccca gcaggcgcca attcgcgcca accagcgcgg
    19501 cacccgctgc gggccagtcc accttgcgca ccgccagcca gccgaatact ccggccgtgc
    19561 tgagccctac cgcggtcacc agccatttcc gcaactttcg tgccctgccg aagagtcacc
    19621 gcgccagttc ggccgcagac cgccggccga acttccgatc ccacacggat tcagtctatt
    19681 ggctgctgcc tggccgtcaa ctgacgtggc caggcgaatt atttcggggc gaaccactct
    19741 gcggccgccc gccagtcccc aagctcgtgc agatcgattt gaagttcgcc gcggcgctgc
    19801 ttggtcggcc ggtcaggcga gtgggtgacc ggcaaatcgc gcatgccgcg aagctgcggc
    19861 agcgcgccgt cgcgcgtggc cgcccagatg aaatgcggcg ggctccacgg atgcagcgta
    19921 gtggccacgc cgaattcgtc gtacaggacg cgttcggccg cgcagtgagc ccagtgcggt
    19981 tgaatcacgt gaatgccgtt tatgttctgc gccacgctct gcgccaggat ttgctgcgtt
    20041 aggcggagtt cgagcgcttg tggtgcgatg attccgcggc gcgtctgttc agagcacacc
    20101 gttgccgccc gcgcggcgac caggaccatg agcgcgctga gtatgcctgc gtgcggcacg
    20161 acgccgcgca aggcgaacgc cgccagaaca accagcagcg ccgccaggct gaagagcgag
    20221 cggtaggccg cccagttatc ggcgatcatc aggttcggca ggtacactat cggagcggct
    20281 gcgagcaagc ccacgagggc cgtcaccgaa cgccattccc ggcgccagcc atagcagatg
    20341 atcgccagta tgacgattac cacgacgatt gttgacacag tcgtgccggg cgcaagctcg
    20401 aatagcgcgc tggcgttacg gagcggctct tggatgaacc actgcaactt cgcgccgacg
    20461 ttggtcacgg ccgaagcgcg tgggttggga gcgctttgca gaacgatgga taagtacggc
    20521 agaagggccc cagccgcgaa tatgagcagg ccgcggatgc cggtggcgat cttgcgtcgt
    20581 ggctcgacgg tgcgcgtggg aagctggtcc agaatgagcc ataccaggta gaacatggcg
    20641 caaggctgat acatccagaa cgtggcgctc aggctggcaa tggcgcccag tccgcaaacg
    20701 atgcccttcc agagcggctt ccaggtcatc tgttgttgaa accagaatgc cagccaagtg
    20761 atcagcaaag cccacgacaa ggcaaagagc tgagtccagc ccactgccag cgcggcacta
    20821 ggcgtgctgc agatcaggaa ggccaaagcc accgcttgcg gcccggtcca gccgcgccgc
    20881 agaacaaacg tcgccaaggc cgcgcccaac ccggccatac ccagaaccgc ggcagcccgc
    20941 agatagctga aggccgcaat cgtgtcgatc tgcgcaaaaa ccgccggata gagcaaaccc
    21001 aagataaagc gcccgcagcc gaacagtaca gatcgtgaca cttcagggtt gtgatgggcc
    21061 atatataggc aactgtagtc atcagacata ccgtaagttg tcatcagaac cggcaaatgg
    21121 agcacaacaa ctgcaacggc cgcacccacg ccggccgtcc aggcctgtcc gcgcgggcca
    21181 ggagtgaagg tgctgcgcgt gcgcggagta accagtagca acgggattac ggcgattgcg
    21241 aagaatacgg acgcgcccgc cagtgcgcga acgaggcgaa tcattccggc cggccaaacc
    21301 agccccagtc gtaccggacg gggcggttgc acgtccaccc agaccgggtc ggacggcagg
    21361 ggcaagcccg gaccggcagg aacctcgatc accagctcac gcgcctccgc cggtattcgc
    21421 gctgccagcc tgtgccactg cgcgtggccg gaattgcttt gggctggaaa ggtgaactgg
    21481 gttagggcag ggccgtccgg ctgtcccgac gcacgtatga acacactcac cgggttggcg
    21541 ttcgcggcag agtcagaagt atgcggctca gggggaaagg gccgggcaat ccacaccgaa
    21601 agcgcgtgct cacagttcgg caaacgcgac aagtctgcga aaactcgatc gctggcgccg
    21661 gccgtggcct ctccgcgctg aagctcaact gcggtcctgc tttgccctcc tgtagtggct
    21721 gtgaagggcc tcacccaacg gcggccctcg gtgtccgcct ccctggtcaa aatcaacggc
    21781 tgctgcgccg gcaggcaggc ggcggcgacc gcgaccagaa tcgcgcctgc ggctattgtc
    21841 gttagtgacc agagctcgtc ccggcgtcgc ccggcccata gcgccacagc cgccgcgagt
    21901 gagtagactg tggccgtaag caagaggctg cccagaacat tttcgggttc aacggtgctg
    21961 gtaagcacgg ccaggcgcac cgcagtcgcc ccggcaatgg ctgctacagc tactttgcga
    22021 tcatttcgga acactgattt tgcgattcct tccgcgctcc agggccggcc gccggttggg
    22081 catccaacgc cgcgcactgt gatgggtctg cttcgtgcag attcgagacg caggagcgga
    22141 caaccgcgtg ttcgccggcg gccagcgact cgaacaggcg gacatagccc tggtaggcgt
    22201 gccggcgatc cagacgccgc gccagatcgg gcggtatcgg cggcggcgga gcgcattcgc
    22261 tgacggccag catggccgcg gacagatttt cgacaaattg cggctgctct tttgccaaga
    22321 gcctggcaaa tgactcggcc ccgtctatgt cgagaatgga ggcatacggc gggggcacta
    22381 gccggccacg ttgcgggtca accagttccg cggccgcgcc gacgtcggtc gccgcgatcg
    22441 gcagtcgggc atagagcgct tcggccagcg acagactcca gccttcgaca aatgacggca
    22501 atacgaatgc gtcgcacagc atgtacaatt gctgcggatc cggccggtgt cccagccaga
    22561 gtatcgcgtc ggccacgcca agccgctcgg cctgttcccg aacgcgctcg gcatacgcct
    22621 cgtccatgat ggggcccagc agcgccagct tgaggtgcgg gttatgttgc cgtgcccggt
    22681 gcagtgccgc gaccagcagg ccttgggcct tgggtgggta gatcgaggcg acgttcagaa
    22741 atacgaaatc ctgctcggcc aggcccaacg ccagtcgctg agcggcgcga tcggtggccg
    22801 cgcgggccgc gtccagcttc tcgaggtcga ttccgttcgg aatgatgagc atgcgttgcg
    22861 gcgacaggcc gagactggct tgtgcataat aagcggcggc tttggagacg tagacgtatg
    22921 cggtggtggc ggcatcggcg cggcggtacg cggcgcgcat gtccgcgtct agcccgacgt
    22981 aggcgctatg gaccgtctgc acgaaggggc ggccggcctc agcgaccagc tcgacgccga
    23041 acagcgagtg gtgcgcatta acgagatccc agtcacgcag caagtcgcga tacgcctgtg
    23101 cccggcccag ttgcggcaga tggagtattg gcagaccggc gcgccgcgct tcaaaagctg
    23161 cagtcccctg gcgacccaac accagcaggc ccactgtcat ctgtgcggcg ctcagcgttg
    23221 cggccaggtc gagcatcacc tgctctaacc cgccctgttc gaagtgctcc acttgcagca
    23281 gtacccgcaa cgggcggcgg cagctgtacg attccgactt tggcaaagct ggagctttgc
    23341 actcccgctc tgtaaacggt tctcgactcg cgtgcggtaa ggtacgcgcc agtgagcgat
    23401 cggagtgcaa acgctgccgg agctgacgta gggcgtgcat gcagcgtcta ccaaggcgct
    23461 cgcgccaact gccgtgcggg aagacgaaaa accgcagccg gtatagcgtc tgcagcaggc
    23521 gccagccggt gctgttgtaa atgccggcca attccgccga aacgcggttc agctccgcgc
    23581 agacggcgat gcccgcctgc tcggcgcggc gcaggcgctc atcgtcagag atcttctcgc
    23641 tgccgacggt cagcccggca cgctccaaga acgccgcggc cgcgccgcca ccggtctgtt
    23701 ccgcgctgag ccgctgataa tcgcgccaaa tgctctgcgc ctcgcgccag ctcgcgtcca
    23761 aagcctcgtt cacccgcacg aattcgatcg cttgtgcggc ggattcgcgc gccgcggccg
    23821 gcgggagcac ggggcgattg gttcggtttc gcaacgtgcg cccgcgttcg agcagtttaa
    23881 agaagcgctc ggccattgcc cgcagtgtga agtgcgcctc gatgcgctgc cgcgcactgc
    23941 gccccaaggt ctgacgccgg cgctcgtcct gtatcagctc ctccaaaacc gcggcatacg
    24001 ccgccgcttc tgcggcctcg ccgcgcttcg ggagcagcat gccgcattcc ggcgtgacca
    24061 gttcgcgctg cccgccgact tcggccccga ctaccgccaa tccgcaggcc atggcctcgt
    24121 agatcgtcag agcgatgcct tcccatagtg aaggcaggaa gaagatgtcc gccgccgcca
    24181 gaagttcgcg cagccgctcg gccgggacct cgcccaatag ccgcacgcgc tccgtcagcc
    24241 cgtgctggcg aatgaactgt tccacccagc cgcgctcggc gccgtcgccg gccagcagcg
    24301 cccggaaagg ctgcttgcgg gccgatagtt tgtgcaatgt cgcggccagc acgcgcggct
    24361 gcttctcggc atccagccgc ccggcgaaca gtatacatac ctcgtccggc gccagggcca
    24421 gctcgcaccg cacacgctgc cgcgtctgcg gacacgggcg ccagagctcc gtatcgatgt
    24481 tcagataaag cacttcaatc cgcgccgcgt cgccgccgcg ctggacgata tagcgtttga
    24541 gatgctcgga agtgaccagc gtcaaatcga cgtagtcctg gtagccggcc gcgtaccgcg
    24601 cgtagccgcc atttttccag tgcggcgcct ccgagtggca caggtcgaga tagaccggct
    24661 ccggacaggt ggaacacaga tatggcagca gcaggtaacc aagctcactg ccactggtca
    24721 gcacgaactg cggttgccgt gaggcgatca ggtagtgcag gaacgccgga taggcgggca
    24781 atcggaggaa cttcggcagc acgaggacgt ccggcgtgaa gcgggtgaac tcgcccgtcc
    24841 acctggggtc ggcgccgtgc aaggccgcaa cggttacttc ccagccacgc tgccccagtt
    24901 gcttcagtag ctccagcgcg gctcgttcgg ctccacccag cgacaggcgc ggaatgatca
    24961 tcagcagccg cggggcgctg cgcgtcagcc gatttaggcc agtgggggcc ggggctaagc
    25021 tctcgaacgg ctcaggtgcg cgccgggggc cttcaggaag gccttttttg agagagtacc
    25081 aacgtttggg cggtgcactg gtcatggcac ccggcgggcc cgcacttggc gctggcatgc
    25141 ccgcgcttgg cgcttgggca tggcacccgg ctggcatgcc cgcgcttggc gcttgggcat
    25201 ggcacccggc tggggcgtgg cacccgtcgt cggcgtgctc gacatgtgtt tcgaagaacc
    25261 agtagagctc ttcaagcgcg gtgggttcga tctccgcgcc ctcgtcgagc tgccagacgt
    25321 aggcaccgcg ggccgcggca gaaattgcag cgcgtgctcc cgttccgccg ccttggggca
    25381 agtctagaac acggacgcgc ggatcgccct gccggtattg gtcgagaata atctgggcgc
    25441 cagggtccgg ggcgcgctcc aggatgagta gccactccca cgcctggaaa ctctgtccaa
    25501 agagcgaaga cgcggttctt tgaagggtgg cgcccccatg gcgaacggtc gtgacaattg
    25561 acaccgccgg cggcgccgcc agatccgcgg gcgcatagcc gaagttcggc acgccgtgta
    25621 atccgggcga gtcgcgggtc gaaccggccg aagctgtgcc ggcctcagat gacatccgcg
    25681 aaccctcgct tggttttcat gaaccaggca tagccaagct gcatgactat cagcgagagg
    25741 gccgtgacga tgcccagcca gagccactcc agcgactgac cgggggtgcc catgagcgtc
    25801 acgcgcgcat tttcaaccac gactgtcagc gggttcaggc gcatcacaaa ctgcacattg
    25861 gggtgcgcct gcaatgcggg catatccaag gggtagaaga tcggcgtcat gaagatgagg
    25921 atttgaataa taacggtgat cgcctgcccc acgtcgcgca cgtacacgcc gatagatgcc
    25981 aggaaccacc ccaaccccag gcacaatagc agcagcggta taaatgtcag cggataatag
    26041 gccagtgcgg gcggcaaacg ctgctccaac agcactatgc ccgtcagcag caccagcgag
    26101 ctgatgagcc cttgcaccag cgccgtgagg agcgccactg ccggcagaat ttcgagcggg
    26161 aagacgacct tcttcacata gttagcgtgc gcgacaatca gcgccgcaca cgcgctcgcc
    26221 gtctcggcga aaatattgaa cagcagcaac ccacagaaca gcttcaacgc gaagcgtacc
    26281 tttgattcat cgcccaaccc ccaccgcgcc tgcagcacaa agccaaacgc gaacgtgtac
    26341 accaccagca tcagcagcgg catcagcagc gaccacaaca ggcccagcgc ggagccgcga
    26401 tatctcagca acaggttccg cttcgtgagc tgcccgataa gctgccggtg ccgccagagc
    26461 gtgcgcggaa tgaggtccgg ccgcaggtaa tacgccactg accgcgattg atgaacgtaa
    26521 gtttgaacct gcgcgacgct cacctcttca ccgctcacat accgtccagc tcggccccgc
    26581 acttcgcgat ccgcgtttcc gcgtttctga gagtggacga acaaaacgca gaccgctgct
    26641 gagagcccag tatccccgag cggccgtacc acttcaaccg ccgtacgaac agccctgctc
    26701 agccgtccag ttcggaaggt tgctcccgcg gccgctcggc aaccacggcc gatagaaatt
    26761 gcctgtgcga ggttctccat cgagcgggcc gcctccagcg gacaacctgg ctcgttatcc
    26821 ggcgacggct gc
//
LOCUS       DAOSYS010000013        26763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1489_length_26763_cov_5.212820, whole genome shotgun sequence.
ACCESSION   DAOSYS010000013 DAOSYS010000000
VERSION     DAOSYS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26763
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_26763_cov_5.212820"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLU99_01615"
     CDS             complement(<1..742)
                     /locus_tag="PLU99_01615"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO15781.1"
                     /translation="MTDVAADSLFARLKADNRLPSPPGTAIRVIELCRCGSVELRDIA
                     TVIMSDPALSARVLKFANSPLLRTPRAVASVREAVLFLGLRTVKNIALGFSLASPSFE
                     PICPEFDLQRFWRDSFLIAVTARRFAQHLGTADREEAFTAGQLARIGQLAFARGAPDE
                     YSQVLRLVAEGLPLVEAERKILGTDHTQLGTRILTEWGLPDVLVRAVERTGRTGLRPD
                     SQEDALAHIVHLALEVLPVFSHKGPLSEL"
     gene            complement(751..1131)
                     /locus_tag="PLU99_01620"
     CDS             complement(751..1131)
                     /locus_tag="PLU99_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15782.1"
                     /translation="MRKAFPEYLAEQGVISPDKLRAALERLHASAEPIGSIAFSHGLL
                     SCQDIDCILDEQRVSRLPFGQIAAQRGLLTEAQIETLLQIQQVRAASEIAEALALSGV
                     CPPLVALEYLGRFLVTLAAKGVAV"
     gene            complement(1334..4534)
                     /locus_tag="PLU99_01625"
     CDS             complement(1334..4534)
                     /locus_tag="PLU99_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO15783.1"
                     /translation="MTHVVGEKTLRVLALRPSRAGAPLEASGIPTKTCRSVEELCTEI
                     RRGAGAVVVDEEALHSEAVEELKRELSAAPAWSELPVIVLMSPAADEARCANIARGLG
                     PRAHVTFLSPGPQWALLSAVRTAMRYRQRQYDYGDRTPEQAFQAGEEFQRRILESSAD
                     CIKTLDLQGRILTMNPRGRELMEVQDLRPFIGKPMPELFAEAHREAVRAAIAQAAAGQ
                     VGKTQAYCPSACGTPRWWDEMITPILGPDGRPERLLAISRDITQTKMAEQALRESELK
                     FRLLAEASRVMVGIVRDRRFLYVNPFLCENSGYTREELLAIDIAELIHPEFRELVLDR
                     ARRRLAGEEVPANYEYLMLTKAGQPRWVEIAPVRIEYRGAPAIIGTAVDVTERKAAEE
                     AVRQNAERFRAFFENATVGAAQVDMSGRFIAANDYMCRITGYSREELLQLSPRDLTHP
                     EEREADWDAYLRMRGGEMHTYRGQKRYIRKDGRVIWVEVTAGRMHDATGRPVGTAGII
                     QDITHRKHAEQEIRHHADGLRLLARTATQLLVTSDPLAELPGILDAVAAHLQAEVYLC
                     YLLTEGQRHLKMHCARGLDDETRRAIDLVELGQTVCATAAQRRTSIVIQNVQADDNPK
                     AALLRTLDLQAYACYPLVGDRLWGSISLGTRSRTSFSQDELDVVRAVADQAVLALTRQ
                     RLDAELKRRAEELDAANRAKDHFLAVLSHELRTPLTPVLATASMLAADVNLSPALREG
                     LDVIRRNTQMEARLIDDLLDMTRIARGQVELDRRPVELRTILERALEVCRPDLEARRL
                     QLSLDLDQTRTVHGDARRLEQVFWNLLNNAVKFTPPGGKLAVRCRAENSYVVAEVADT
                     GTGIEPADQQRIFDAFEQAEHTHRRQFGGLGLGLAICKALVEMHGGTITATSPGKGHG
                     STFTVRLPVAEQPAVQAGAPPSAAPRPAPAGSLQILLVEDHPDTVKIVTWLLQSIGHT
                     VQTAGDIRTGLERLGQQKFDLLISDLGLPDGSGVDLVSEMRRRGYELPAIALTGYGQQ
                     EDVQRTRAAGFAKHLTKPVELEQLEEAISQQFASQR"
     gene            complement(4673..5530)
                     /locus_tag="PLU99_01630"
     CDS             complement(4673..5530)
                     /locus_tag="PLU99_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO15784.1"
                     /translation="MTTATYDLDMQKMFAAVSDLYARYWGNYFHFAIFENEAQPWEQA
                     LENTHRAYMNALKVGQARRVLDIACGRGGFARLMAENTPGEVLGIDISESQLSHARRL
                     ERPNLRFRRHDAMKIDELGQTFDAVSFLDAACYLPDKLLAMRRIRHVMEPGARLLLVD
                     WCRRERLGAVQQDLVLAPFMKYWGVPYLETRGRYEHLFSRNGYRLLHVDDLNDRVAPN
                     WELGYRRALQALAELTARDLPKLIWNGMRLGKEGIELIKDQFPAAVYIKIGFDIGFLR
                     YVYFLVQAA"
     gene            complement(6058..9147)
                     /locus_tag="PLU99_01635"
     CDS             complement(6058..9147)
                     /locus_tag="PLU99_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15785.1"
                     /translation="MRRDALIIVTGLLLCAPGAASAFQVGDLNCDGAVNVFDIDPFVL
                     ALTDPSGYAAQHPGCDLLLGDANYDGAVNVFDIDPFVSLLTGGPAAPVAIQLAGNALT
                     DYPYFEFVRAFHQNAPIQVAIDPTLHPEIVGRTADIYVVEKKSAGEWAVDPTLADVTV
                     DGLLTVTFAGDTIQENTFQVTGPNELNAAVYEEWTGDYTGLGHGYDVVVDMNRNGRLD
                     GGDYIDGLGREAGLYVCHDTTAPGPLAVTNVTYSVGTIFGIPSGLGGQKLYYPTNIDE
                     MGQLPLVVVSHGVGHDYQWYGHIGNHLGSYGFIVMAHQNNVSTVETASLTTCGHTDAF
                     LHLLPGIAGGALVGHVDMHRIIWVGHSRGGEGVAIAIHRISFTPHSYEPTYYTAEDIV
                     MVDSMLPVDFNTQQLTYPGSVNYHLWTASGDADVHGGAGVDRAQTFHIHERATRYRQS
                     TVVQGTGHGWFHSGSGGAVFEGPCPINAAGTHLVQLGLMLPMYKHYAHGNIPGSDYLW
                     RQYERFHPPSVPVGIDPCYVVSQEYRNGHEDGTCFIDDYETNEGAGLSSSGGAVTYNV
                     TNLTEGRLDDNNSSFAWSASDPFNGATQDSTDWWDTGRGVVFDWNGVNRYYEWAVVPE
                     LRNFAAWKYLSLRGAQGTQHPYTMATAGLLTFTITLRDAAGNVSSINTGAYGGGFGMP
                     YARQGGWHNEMRRIRMRTSDFLANGSGLDLSNIVAVRLNFGPSWGTSQGRIVIDELML
                     DNDLPSAFTPLTLSLIGAPPDFVPPHEPTTLNVEIIEGDDQLVAGTALLYYRYDGGAW
                     QSTPLVPVGGELWNATLPAPACGDLPEYYFSATGQITGEVLAPAAGATAPFSALVGTY
                     IEILEDNFQSDLGWTVDSAAGITSGVWQRAIPFGADDGSPPEDFDASGWCYVTDNRAT
                     FDVDGGPTYLTSPVLDLSGADDPMLRFAAWFTCDDPIPPAQDFLDVHVSSDGGATWVQ
                     VAHISNFDGWVLHEVHLADYIPLTAGFQVRFTAIDLPNNSRTEAAVDAVKVFDVSCE"
     gene            complement(9350..9961)
                     /locus_tag="PLU99_01640"
     CDS             complement(9350..9961)
                     /locus_tag="PLU99_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15786.1"
                     /translation="MKTVLVSVCLLTATSISMAAPPVPNTPAAVDEVVYARPFSLNDG
                     FQYQWASEPFQVTKGTILVLKVDPKLVVPREIGEPVLYVGHQAAMRLNQGDQSGYVIA
                     VVPGDVDLTKDLIWFGAAELPDRINAAHVKAERRQAEKAGLKPLPADKAKTATDAGGR
                     PANAQDMSALLRDTIGGLVEQYSPQEKHLAESWRLPVVQPVTP"
     gene            complement(10779..12269)
                     /gene="gatB"
                     /locus_tag="PLU99_01645"
     CDS             complement(10779..12269)
                     /gene="gatB"
                     /locus_tag="PLU99_01645"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOO15787.1"
                     /translation="MTNATELTVEPIIGLEIHVQLATRTKMFCGCPLEFGAPPNTRVC
                     PVCQGLPGALPVINRQAVELAVRAALALNCRIATFTKWDRKNYYYPDLPKNYQISQYD
                     LPISSDGYFELTCDGRPRRVGIIRAHLEEDAGKNVHDFPGYTGVDLNRAGTPLLEIVT
                     QPDLHSADEAREFAVQLQRLVRYLGVSEANMQEGHMRFEPNINLRIISGGQEFRTPIV
                     EVKNLNSFRSLYLAVQYEIDRQLEQWRETGETAAMGNKSNRGWDDVKEITLPQREKEE
                     AHDYRYFPDPDLVPMTFDPAWVGQLQSEMPELPIRRTERFVAEYRIPAQDAPALVDDR
                     RTADLLDSAAAAGGDKVTLAKHFLSFWAKHANERGTTVAGLGIAPQRLAELANLVQAG
                     KVNATAAAQIAEAMLSSSDSPTALAERLGLLQSRDTDQIAAWVDQALAANAQAVQDAI
                     ANPKKIKAARGFLTGQVMKLSGGKADPKIVGELIEKKLAEKTGGNH"
     gene            complement(12578..13486)
                     /locus_tag="PLU99_01650"
     CDS             complement(12578..13486)
                     /locus_tag="PLU99_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO15788.1"
                     /translation="MPSALAEIAVGGFHVGGFSTAGEETFFVIPEMNIGFDIGRGRRE
                     VLAVDNIFLSHGHMDHAAGVAYYFSQRMFIDNRPGNLYAPEPLVQPIRRLLQVWGEID
                     GNEPPANIFPAYAGEDIQVRRDLIVRPFEVNHPCRRRVRGAYPSLGYVAIEVRNKLLD
                     EYQGLEGPELVALKKKGVAITRRVELPLVAYCGDTAPGDFFKLDYVRDAKILLLECTF
                     VDPDHLERARAGFHIHVSDLREILPQLRNERIVLCHLSRRTALPEAREIIARELGDLF
                     SDRVSLLMQHRARPRRRPPDPGPAGK"
     gene            13670..14182
                     /gene="nrdR"
                     /locus_tag="PLU99_01655"
     CDS             13670..14182
                     /gene="nrdR"
                     /locus_tag="PLU99_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOO15789.1"
                     /translation="MRCPFCKADDDKVVDSRLTEAGRSIRRRRKCLRCGRRFTTYERV
                     EETIRLTVIKRDASRVPYDREKMREGIVRAAYKRPISAERIDQIVDEVEEYLVSHFEK
                     EVSSQTIGERIAAVLRRVDKVAYVRFASVYRQFEDVGDFIEEAQDLIERSADDIPGQR
                     DLFQEEPREP"
     gene            14179..14781
                     /locus_tag="PLU99_01660"
     CDS             14179..14781
                     /locus_tag="PLU99_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15790.1"
                     /translation="MKPKKVLVITKRDGTLECFSASKLQSCLRRVLQAGGEEWRMAGP
                     LSQAIAVHLRQLEDSEVPTSEYVYGCARAALTQTGLGYSAEILKAHRRLREARRARVR
                     VYDPGECVRAPVRWRKGAIVETLRNVYDLGQPVARYLAGLIEERVFELGYRLLSKPFI
                     AELVHNEVLAWGLLFERTSRSAPQVERPNSVAGSGPPKED"
     gene            15181..15789
                     /locus_tag="PLU99_01665"
     CDS             15181..15789
                     /locus_tag="PLU99_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15791.1"
                     /translation="MMKLPALENSSCYRGLYVYDFGEWTAVGYTAEEIAVLLESEAYA
                     HGQVYRIQRIQPDGSMELRGVSAERFKLESGLFFWRSTRDEADADFRSLLSAAQHSPP
                     PCRAALQMSERLDACDRYQFVTALIFPAEYEDDVAAWLLRLGYVGGDVAEGGVSHVTN
                     YYELARRVLAAEQLTSAAPIRPRSAEEVLAAVRQPVQRVMVA"
     gene            16027..16710
                     /gene="plsY"
                     /locus_tag="PLU99_01670"
     CDS             16027..16710
                     /gene="plsY"
                     /locus_tag="PLU99_01670"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOO15792.1"
                     /translation="MVATFAFALGAYLVGAIPFGLLVGLSRGVDIREHGSRNIGATNA
                     GRVLGRRWGLLCLALDILKGFLPVLAAGRFLPGGEASSTRLLQLLAVAVAAVLGHIFP
                     VYLKFRGGKGVATTVGVALGIYPYYTIAMLVALGCYALIRWTTGWVSLGSLAIAVTFP
                     ASLFAYLHYSGRRLSDFWPLLAVAVLLGALIIVRHRTNIARLLRGQEARVTSGAAGAA
                     GSEPRERLH"
     gene            16948..17421
                     /locus_tag="PLU99_01675"
     CDS             16948..17421
                     /locus_tag="PLU99_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO15793.1"
                     /translation="MISQTTEYALRAVVWLAAHPDRPLTAQQIAEATRVPAGYLAKVL
                     QGLSRAGLLHSQRGLGGGFTLARPPSNVSLWEVVQAVDPIRRITQCPLGLELHANRLC
                     ALHQELDRAIAQVEKDFSRCSIASLIEERGELVPLCAVAPKGKGRTKTVAKRAKR"
     gene            complement(17532..19643)
                     /gene="uvrB"
                     /locus_tag="PLU99_01680"
     CDS             complement(17532..19643)
                     /gene="uvrB"
                     /locus_tag="PLU99_01680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOO15794.1"
                     /translation="MSEFRIVSPFAPAGDQPQAIEKLVAGFQAGRKYQTLLGVTGSGK
                     TFTMAHVIARLGLPTLVISHNKTLAAQLYEEFRELLPHNAVEYFVSYYDYYQPEAYIP
                     QRDIYIEKDASRNDDLDRLRLAATSAVVSRRDVVVVASVSCIFGLGSPDEYKASVIDV
                     RVGQELDRDELLKKLVRLQYERNHTELQRGSFRVRGDVVELAPAGEEYAVRIELFGDQ
                     VDRITLIHPLTGAALQERSQVYIYPAVHYVLPEDNLERALQTIQEELDWRVMQLRHEG
                     KLLEAQRLLARTKYDMEMLAEVGYCSGIENYSRHLNQTAEGAKPYTLVDYFPKDFLLV
                     LDESHVTVPQIGAMFNGDRNRKQVLVEHGFRLPSCLDNRPMRFEEFESMWSKVLFTSA
                     TPGPYELKKCGGEVVEQIIRPTGLVDPEIEVVAARGQVPDLLRRIQERAAAGERVLVT
                     TLTKRLAEDLSSYIQQAGLRGRYLHSEIDTLERVEILRELREGKFDVLVGVNLLREGL
                     DLPEVSLVAIMDADKEGFLRSETSLIQTIGRCARNVNALVVLYGDKVTPSMQKAIDET
                     NRRREIQRKYNAEHNITPDTIKKAIRAGLMNEVAAMRRVREVIHQDEEERDRDELIKE
                     LEQEMLQAAEELDFERAAALRDHIKELKESPQVKVAASSVRPLHEPAARGTGNARRTP
                     SQAAASWRPKGQHRRPRPRGK"
     gene            complement(19665..20549)
                     /locus_tag="PLU99_01685"
     CDS             complement(19665..20549)
                     /locus_tag="PLU99_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOO15795.1"
                     /translation="MASSAALSLGGTPGKPWILVLATVVAALIAPSVQIAYQWERGVL
                     LRFGKFVGIRGSGVFGVLPIIDKVAQYVDQRIRVTDFRAEQTLTKDTVPVNVDAIAFW
                     MVWDAEKSVLEVEDFEQAVVLSAQTALRDAIGRSELADLLSHRERVGHEIQQVLDAKT
                     NPWGITVQSVEIRDIIIPKGLEDAMSKQAQAERERQSRIILGTAETEIAEKFSRASEA
                     YRNNPVALHLRAMNMVFEGLKQKGSMIIVPSTAVETMGLGALGGLTAFNQAARSGAEA
                     GRGEQKPEETSGAPESAE"
     gene            complement(20855..21739)
                     /locus_tag="PLU99_01690"
     CDS             complement(20855..21739)
                     /locus_tag="PLU99_01690"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOO15796.1"
                     /translation="MTDRPHTAIVIPDPPRVVPSRLYLRRARCMFFLGISFLAMSPAG
                     VLIMLIVHSSIGGGLTAPLPDLLLNANHSIATGTVTSKKLITAVHMNSEHPWRIGFHY
                     DLPGGASVAGSGFTYDQDLGNRIQPGDQIEIEYDPSNPTRARPAGGYVGSAPPWVQLL
                     LHGMFGLIGVLGAGLLIATYMTERRDRKLLTYGIAAEGTVVRVRCIGTINFGTKHPYD
                     VYYQFRDPWGREITGRDRTYSYAWTEGLQPGERVGVVFNPRDPHENALWLHGKELERL
                     GGIDPALLRPGIHSGRTG"
     gene            complement(21736..22119)
                     /locus_tag="PLU99_01695"
     CDS             complement(21736..22119)
                     /locus_tag="PLU99_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOO15797.1"
                     /translation="MIQFAIDPKSGVPFYRQIIDQIQFAIADGRLSRGDRLPTVRQLS
                     VDLKINPNTVARAYQELEIKGVLDSQMGTGTFIGSAKVEVSALERKRMLHQICTELLS
                     RASAYGFTTSEVANALRELTEESRK"
     gene            22365..23270
                     /locus_tag="PLU99_01700"
     CDS             22365..23270
                     /locus_tag="PLU99_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOO15798.1"
                     /translation="MTARILHGAAIAAEIRAEVTRAVARYNPVFGPIKLCAILVGTDA
                     QGRLYADSQRKLAVVVGVQYELIELPETARQEQVHAEIDRLNADPSVTGIILQLPLPP
                     HIDAAAAQYHIDPYKDVEGVNPANIGWLFYGEPIIAPCTALAVNEMIRRSGVAVRGAE
                     AVVVGQSRIVGKPVTMFLLEQMATVTGCQITTQDLASHTRRADILVVAVGRPQLIGAE
                     HVKPGAVVIDVGINRITETDAAGKKVRRTVGDVDFEAVAPIASAITPVPGGVGPLTVA
                     MLLRNTVEAARKQRERAGTVCTREP"
     gene            complement(23354..23749)
                     /gene="mnhG"
                     /locus_tag="PLU99_01705"
     CDS             complement(23354..23749)
                     /gene="mnhG"
                     /locus_tag="PLU99_01705"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOO15799.1"
                     /translation="MTLLVQILMVVLAVAGTALVLLGAVGILVLTDVYTRMQAASKAG
                     SLGAACILLSAAMYFSELELVVRALTALVFLMLTIPVAAHVLGRAAYLAGVPLAKESV
                     MDELHGRVEGRTNSPGGQDEPNPPDPAPE"
     gene            complement(23746..24039)
                     /locus_tag="PLU99_01710"
     CDS             complement(23746..24039)
                     /locus_tag="PLU99_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOO15800.1"
                     /translation="MEGVAPSGPGWATFWEVLVGLAMLSIIVAVVRLARGPTLADRVV
                     ALDLIATIAVVTAALSSIAYSQPVFLDLAVVLTLVTFVATVAFARAIQRTAQP"
     gene            complement(24143..24613)
                     /locus_tag="PLU99_01715"
     CDS             complement(24143..24613)
                     /locus_tag="PLU99_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOO15801.1"
                     /translation="MIMFAANLVLALIWWAATVRGGWNLIFGFAIGYAVLWWMSPILG
                     KSRYFEKLPQLIRFTGFLAWQLVHSSLRVARDVVTRKTYRRPGIVAVPLDAKTDAEIT
                     VLALFISLTPGTLAIDVSDDRKVLYVHEMFITDPEEVRRQLKAGFERYVLALLR"
     gene            complement(24610..26178)
                     /locus_tag="PLU99_01720"
     CDS             complement(24610..26178)
                     /locus_tag="PLU99_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO15802.1"
                     /translation="MKLLLVAPILIPLLTAGVTVAFWRPARFRAWCGVVGAAALLAAG
                     GLLLAATADGQVRTVQIGNWPAPFGITLAVDLFSALMVLITGIIGLMVAVYSLADVDI
                     SRQRYGYFPLFHILLAGVCGAFITGDIFNLYVWFEVMLMASFLLLALGSERAQLEGAF
                     KYVTINLISSMVFLTGVGILYGTVHTLNLADLALRVPEMMDRHGHLVRACGMLFGVCF
                     GLKAAVFPLFFWLPASYATPPAAVSAVFAGLLTKVGVYALIRVFTLVFGSIPGIYALI
                     LVVAGLTMLVGVLGAVAQFEMRRILSFHIISQIGYMIMGLGLLVSSDPGVRTLGLTAG
                     IFYIIHHIIVKTNLFLVSGTVRQLGGTYDLRSLGGLVRAPLLSALFLIPALSLAGIPP
                     LSGFWAKLGLIEAGFLAGQNTIVIVALLTSLLTLVSMMKIWNEVFWKSAPQDASGPAE
                     PMAPGQWAARFIPIALLAGLTVLIGLFPAPLLTLAGRAADRLLNPAAYISAVNLHDAA
                     DSGLSTIEISEDTP"
     gene            complement(26175..26510)
                     /locus_tag="PLU99_01725"
     CDS             complement(26175..26510)
                     /locus_tag="PLU99_01725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017035313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOO15803.1"
                     /translation="MAIVLGGLFAGGLYLMLARSITRVLLGLVLIGHATNLLIFTAGG
                     LVRGRPPLVPAGQTEPAAGYADPLPQALILTAIVISFALTAFTAVLVRGTYQAAGTED
                     SDGLRSTDT"
     gene            complement(26522..>26763)
                     /locus_tag="PLU99_01730"
     CDS             complement(26522..>26763)
                     /locus_tag="PLU99_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOO15804.1"
                     /translation="AARRLLRVHPLTLIGAGLLLALASGCAGLFLGRPFLTGLWTGVT
                     LPEGSEFGSVLFFDLGVYLMVLGAALLILLTLAED"
BASE COUNT         4869 a         8369 c         8529 g         4996 t
ORIGIN      
        1 ctagctcgct caaggggcct ttgtgggaga ataccggcaa cacttccagc gccaggtgta
       61 cgatgtgcgc cagcgcgtcc tcttgtgagt caggccgcag gccggtccgc ccggttcgtt
      121 ccacggcccg taccaacacg tcgggcagcc cccattcggt cagtattctg gtgcctaatt
      181 gcgtgtgatc tgtgcccagg attttccgct ccgcttcgac gaggggcagg ccctctgcga
      241 ccaggcgcaa gacttggctg tattcgtcgg gagcgccgcg ggcgaaggca agttgtccaa
      301 tccgcgcgag ctgcccggcg gtgaaggcct cctcgcgatc ggcagtacca agatgttgcg
      361 cgaaacggcg ggcggtcacc gcgatcagga agctgtcgcg ccagaagcgt tgcaggtcga
      421 attccgggca aatgggctcg aagctgggag aggccaggga aaagcccagg gcgatattct
      481 tcaccgttcg caggccgaga aacagcaccg cctcgcgcac ggatgcgacg gcgcgggggg
      541 tacggagcag cggcgagttg gcgaatttca ggactcgggc cgacagagcc gggtctgaca
      601 tgatgacggt agcgatgtcg cgcagctcaa cagagccgca gcggcagagc tcgatcacgc
      661 ggatggcggt accgggcggg ctcggcaggc ggttgtccgc cttaaggcgg gcaaagaggc
      721 tgtcggcggc cacgtcggtc atgcgctccc ttagacggct acaccctttg cggccagggt
      781 gaccaagaaa cgtcccaggt attccagggc gacgagcggc gggcacacac cggagagggc
      841 caaagcttcg gcgatctcac tggcggcgcg tacttgctga atctgcagta gcgtctcgat
      901 ctgcgcttcg gtcagcaagc cgcgctgggc ggcgatctgt ccgaatggca ggcggctgac
      961 gcgctgctcg tccagaatgc agtcaatatc ctggcaggaa agcaggccgt ggctgaaagc
     1021 gattgatccg atcggttcgg ctgacgcatg cagccgttcc agggcggcac gaagcttatc
     1081 gggcgagatt acgccctgct cggcgaggta ctccgggaac gcttttcgca atgtcgcttc
     1141 cttttgtggc gatgtaatgt cggtcccagg tgctaaatcg acccaaacag gcaggggggt
     1201 gaatctattg ggaggagccc ggtcgggcgc cggggcggcc gggttggcgt acgcgtgttt
     1261 atcggcctgg caccgacctt atcctcggtt ttccggcaat gtcatgcgcg gccaagtcag
     1321 cgtagcgggg ctgtcagcgc tgggacgcga attgctgcga gatggcttct tcgagctgct
     1381 cgagttcgac cggcttggtg aggtgcttgg caaaaccggc cgcgcgtgtg cgttgtacat
     1441 cctcttgctg gccgtaaccc gtgagcgcga ttgcgggtag ctcgtagccg cgccgacgca
     1501 tttcgctgac cagatcgacg ccgctgccgt cggggaggcc caggtcgctg ataagcagat
     1561 cgaatttctg ctgcccgagc cgctccagac cggtgcgaat atcgccggcg gtttggaccg
     1621 tatgcccgat cgactgcagg agccaggtga cgattttcac cgtgtcgggg tggtcctcga
     1681 cgaggagaat ttggaggctg cccgcaggcg ctgggcgcgg cgcggcgctg ggtggcgcgc
     1741 ccgcctgcac agccggttgc tccgccaccg gcaggcgcac ggtgaaggtt gacccgtggc
     1801 ccttgccggg gctggtggcg gtaatggttc cgccgtgcat ctcgaccagg gctttgcaga
     1861 ttgccaagcc cagccccagg ccgccgaact gacggcggtg ggtgtgctcg gcctgctcga
     1921 aggcgtcgaa aatgcgctgc tggtcagccg gctcgattcc cgtgccggta tcggcgactt
     1981 cggcgacgac gtaactgttc tcggcgcgac agcggaccgc caacttaccg ccgggcggcg
     2041 tgaacttgac ggcattgttc agcaggttcc agaacacctg ttccaggcgt ctggcatcgc
     2101 catgcacggt tcgcgtttga tccaagtcga ggctgagctg caggcggcgt gcttcgaggt
     2161 ccgggcgaca cacctccaag gcgcgctcca ggatggtccg cagctccacc gggcggcggt
     2221 ccagctcgac ctgcccgcgc gcgatgcgag tcatgtccag gaggtcgtca atcagccgtg
     2281 cctccatctg cgtattgcgc cgaattacgt ccagcccttc gcgcaaggcg gggcttaggt
     2341 ttacgtcggc cgcgagcatc gaggccgtcg ccagcacggg tgtcagcggg gtgcgcagct
     2401 cgtgcgacag cacggccagg aagtgatcct tggcgcgatt ggcggcgtcg agttcctcgg
     2461 cgcggcgctt caactccgca tccaatcgtt ggcgagtaag ggccagcact gcctggtcag
     2521 ccacggcccg cacgacgtcc aactcatctt gcgagaagct cgtgcgactg cgggtgccca
     2581 acgagatcga cccccagagg cgatcgccca ctaacgggta gcaggcgtac gcctgcaaat
     2641 cgagggtgcg tagcagggcg gctttgggat tgtcgtccgc ctgcacgttc tgaatcacaa
     2701 tgcttgtgcg ccgttgggcc gccgtcgcgc aaacggtttg cccaagctcc accaggtcga
     2761 tggcgcgcct ggtttcgtcg tccagaccgc gggcgcaatg catcttcaga tgccgttgac
     2821 cctcggtgag caggtagcac aagtaaacct cggcttgcag gtgcgcggcc accgcgtcca
     2881 gtattcccgg cagttccgcg agtggatcac ttgttacgag caactgagtc gccgtccgcg
     2941 ccagcagtcg caagccatcg gcgtggtgcc ggatttcctg ttccgcatgc ttgcgatgtg
     3001 tgatgtcttg aatgatgccg gccgtgccga ccggccgccc agtcgcgtcg tgcattcgcc
     3061 cggcggtgac ttcgacccag atcacccgcc catctttgcg tatatagcgc ttctggccgc
     3121 ggtaggtgtg catctcgccg ccccgcattc gcaggtaagc gtcccaatcg gcctctcgtt
     3181 cctcggggtg ggtgaggtcg cgcggtgata gttgcaataa ctcctcgcga ctgtaaccgg
     3241 ttattcggca catgtagtcg ttggcggcta tgaaacgccc gctcatgtcc acttgcgctg
     3301 cgccgaccgt tgcattctcg aagaaagccc gaaagcgctc tgcgttttgc cgcaccgcct
     3361 cctcggccgc tttgcgctcg gtgacgtcga cggccgtacc gatgatcgcc ggcgccccac
     3421 ggtattcgat gcgtaccggg gcgatctcga cccagcgcgg ctggccggct ttggtgagca
     3481 tgaggtattc atagttggcg gggacctcct ctcccgccaa gcggcgccgc gcgcgatcca
     3541 aaaccaactc gcggaactcg ggatgaatca gctcggcgat gtcgatcgct agcagctcct
     3601 cgcgcgtata cccgctgttt tcgcacagga acgggttgac gtacaggaac cgccggtcac
     3661 gcacgattcc aaccattacc cggctggcct cggccagcag gcggaacttc agttcgcttt
     3721 cacgcagggc ctgctccgcc atcttggttt gcgttatatc gcgtgagatg gccagcaagc
     3781 gctcgggtcg cccgtcgggc ccgagtatgg gggtgatcat ctcatcccac cagcgcggcg
     3841 tgccgcacgc gctggggcaa taggcttgtg ttttgcccac ttgtccggcc gcggcttggg
     3901 cgatggcggc gcggacggcc tcgcggtgcg cttcggcgaa gagctcgggc atcggcttgc
     3961 cgatgaaagg gcgcaaatcc tggacttcca tcagctcccg accgcggggg ttcatcgtca
     4021 atatgcggcc ctggagatcc agtgttttta tgcagtcggc gctgctttcc aaaatgcgac
     4081 gctggaactc ctcgccggcc tggaaagcct gctctggggt gcggtcgccg taatcgtatt
     4141 ggcgctgccg gtagcgcatg gccgtcctca cggcgctcag cagcgcccac tgcggccccg
     4201 gcgacaggaa cgtcacgtgc gcccgcgggc caagtccgcg ggcgatatta gcgcagcgcg
     4261 cctcatccgc cgctgggctc atgagcacga tgacgggcag ctcactccag gccggcgcgg
     4321 ctgacagctc gcgttttaat tcttcaaccg cctcggaatg cagcgcctct tcgtccacaa
     4381 ccaccgcgcc ggcgccgcga cgaatctccg tgcagagctc ttcgacgcta cggcaagttt
     4441 ttgtgggtat acccgaagcc tcaagcggcg caccagcacg gctgggccgg agtgccaaga
     4501 ctcgcaacgt tttctcgccc actacgtgcg tcacgccaat cctcgccgcg tgaacacggc
     4561 gtaaagtgtg actcccagga gcgtctggcg ccattttgag agccaaggat aatgctagta
     4621 cggctccgaa atgccttcaa ctgcgcaatt gcgcaagagg gagatgcgca tattacgccg
     4681 cctgaactaa aaagtaaacg tacctcaaga agccgatgtc gaagccgatt ttaatgtaca
     4741 ccgcggccgg gaattggtct ttgattaact caattccctc tttgccaagc cgcatgccgt
     4801 tccagatcaa cttgggtaaa tcgcgcgccg tcaattcagc tagcgcttgc aaggcccttc
     4861 gatagcctaa ctcccaattg ggtgcgaccc ggtcgttcag atcgtctacg tggagcaggc
     4921 ggtaaccgtt gcggctgaac agatgttcat accggccgcg tgtctcaagg tagggcactc
     4981 cccagtattt cataaagggc gcaagcacta gatcttgttg tacggcgccc aagcgctcgc
     5041 gccggcacca atcgacgagc agcaagcgag cgccgggttc cattacatgc cgaatgcgcc
     5101 gcatggccag cagcttgtcc ggcagataac aggcggcgtc caggaaggag acggcgtcga
     5161 aggtctgacc gagctcatcg attttcatgg catcatgtcg cctgaagcgc aggttaggcc
     5221 gttccagccg ccgggcgtgc gaaagctgcg actcggagat gtcgatgccc aacacctcgc
     5281 ccggcgtgtt ttcagccatc aggcgcgcga acccgccgcg cccgcaggcg atatcaagca
     5341 cgcgccttgc ctgcccgact ttcaatgcgt tcatgtacgc gcgatgcgtg ttctccaggg
     5401 cttgctccca aggctgcgcc tcgttttcga agatggcgaa gtggaagtag tttccccaat
     5461 atcgagcgta gaggtcgctg acggccgcaa acatcttctg catgtccaag tcgtaagtgg
     5521 cagtcgtcat cctggcaccg tatcgaaccg tgtgttcggg cgcggcggtt cgattctatc
     5581 cgcgtgcccc gcctcgccgg cgtgccggtg ccatgcccaa gccgtgtggc gggtgccatg
     5641 cccaagccgc gtggcggcgc gggcatgcgt tcgccaatgg gccccatttt cctacgcgtg
     5701 actgtgggaa agtggtaatg tgggaatgtg ggaatgtggg gaggtgccgt cctgaccctt
     5761 cacaccctga taccgtcttc gcacgtccga ccgggtgcgg ccgcgcgtta agggagggca
     5821 tgcccgcgct tacagcttgg gcatggcacc caacgcgctt acagcttggg catggcaccc
     5881 aacggacggc cacccaacgg atgccatcac ggccacccga cccaagcaac ggacgcccac
     5941 ccaacagcta cgacaggcag cgacaagcgc cgccgccgga ccgcactggc taaacggacc
     6001 aggtgcatcg cggcccggcg gcggcttaac cccggaacaa aaacgaacaa cctcgctcta
     6061 ctcgcagctc acgtcgaaca ccttgaccgc gtcgaccgcg gcctcggtgc gcgagttgtt
     6121 cggcaagtct atagcagtga agcgcacttg gaagccggcc gtcagcggga tgtaatccgc
     6181 caaatgcact tcgtgcagta cccatccgtc aaagttggag atgtgcgcta cctgtaccca
     6241 agtggcgccg ccgtcactgg atacgtgcac gtccaagaag tcctgcgccg gcggaattgg
     6301 atcatcgcac gtgaaccacg cggcgaagcg caacatggga tcatcggcgc cgctcaaatc
     6361 cagcacgggc gaggtcaggt acgtgggacc gccgtcgacg tcgaaggtcg cacggttgtc
     6421 agtcacgtaa caccagcccg acgcgtcgaa gtcctccggc ggcgagccgt cgtcggcgcc
     6481 gaacggaatg gcgcgctgcc agactccgga agtgatgccg gccgcgctgt ctacagtcca
     6541 gcccaggtcg ctctggaaat tgtcctccag tatctcgatg taagtgccga ccagcgcgga
     6601 gaagggtgcg gtagcacccg cggccggcgc cagcacttcg ccggtgatct ggccagtcgc
     6661 gctgaagtag tattcgggca agtcgccgca agccggcgcc ggcagcgttg cgttccacag
     6721 ttcgccgccg accggcacca ggggcgttga ttgccatgcg ccgccgtcgt agcggtagta
     6781 cagcagtgcg gtgcccgcca ccaactggtc gtcgccctcg atgatctcga cgttcagcgt
     6841 ggtcggctca tggggcggca cgaaatccgg cggcgcgccg atcaggctga gcgtcagcgg
     6901 cgtgaaagcc gagggcaaat cgttgtcgag catcagttcg tcgatcacga tgcggccctg
     6961 actagtgccc caggacggac cgaaattcaa gcgcaccgcc acgatattac tcaaatccag
     7021 cccgctgccg ttggccagaa aatcgctggt gcgcatccgg atgcggcgca tttcgttgtg
     7081 ccacccgccc tgccgcgcgt acggcatgcc gaagccgccg ccgtaggcgc cggtgttgat
     7141 ggagctgacg ttcccggccg cgtcgcgcag ggtaatggtg aacgtcagaa ggccggcggt
     7201 ggccatagtg taggggtgct gggtgccttg cgcgccgcgc agggagaggt atttccaagc
     7261 ggcgaagttt cgcagttcgg ggacaacggc ccactcatag taccggttta cgccgttcca
     7321 atcgaagacg acgccgcggc cggtgtccca ccaatcggtg ctgtcctgcg tggcgccgtt
     7381 gaagggatcg ctcgcactcc aggcgaaact ggagttgtta tcatccaagc ggccttcggt
     7441 gaggttggtt acattgtacg tgactgcgcc gccggaacta ctgaggccgg cgccctcgtt
     7501 ggtctcgtaa tcgtcaatga agcacgtacc atcctcatgg ccgttgcgat actcctgtga
     7561 gacgacgtaa cacgggtcga tgcccactgg cacagagggc gggtggaagc gctcatactg
     7621 ccgccagagg tagtcgctgc ccggaatgtt gccgtgcgcg taatgcttgt acatcggcag
     7681 catcaatccg agctgtacca gatgggtacc ggcggcgtta atggggcagg ggccctcgaa
     7741 caccgcgccg ccgctgccgg agtggaacca gccgtggccc gtgccttgga ccacggtcga
     7801 ctgccggtag cgcgtggccc gctcgtgtat gtggaaagtc tgggcccggt cgacacctgc
     7861 cccgccatgc acgtcggcat cgccgctggc ggtccacagg tgatagttga cggaaccggg
     7921 gtatgtcaat tgctgagtgt tgaagtcgac cggcaacatg ctgtcgacca tcactatatc
     7981 ctcggcggta tagtaggtgg gttcgtacga atgtggggta aacgagatgc ggtgtatggc
     8041 aatcgccaca ccttccccgc cgcggctgtg tccaacccaa atgatgcggt gcatatccac
     8101 gtggcccacc agcgcgccgc cggcgatgcc gggcagcagg tgcaggaaag cgtccgtgtg
     8161 gccacaggtg gtcaacgagg ccgtctcgac cgtagagacg ttgttttggt gtgccatgac
     8221 gatgaatccg tacgagccca aatggttgcc gatgtgcccg taccattgat agtcatgtcc
     8281 cacgccgtgg ctgacgacca ccagcggcag ttggcccatt tcgtcgatgt tggtgggata
     8341 gtacagcttc tgaccgccga gaccggaagg tatgccgaag attgtgccta cactataggt
     8401 gacattggtc acggccaacg gcccgggcgc ggtagtatca tggcagacat acaggccggc
     8461 ctcgcgaccg aggccgtcaa tgtagtcgcc gccgtccagc cggccgttgc ggttcatgtc
     8521 cacgaccacg tcgtagccgt ggcccaggcc ggtgtagtcg ccggtccatt cctcatatac
     8581 ggcggcattc agctcgttgg ggccggtaac ttgaaaagta ttctcttgta ttgtgtcgcc
     8641 agcgaaggta accgtcagca gcccgtcaac agttacgtca gcaagagtgg gatcgacggc
     8701 ccattcaccg gcggacttct tttcaacgac gtagatgtcg gcggtgcgcc cgacgatctc
     8761 cggatgcagc gtcgggtcga tggccacctg gatcggggca ttctggtgga aggcccggac
     8821 aaattcgaaa tacgggtagt ctgtcagggc gttgccggcg agttgaatcg ccactggtgc
     8881 ggctgggccg ccagtcaaca gcgatacgaa gggatctatg tcgaacacat ttaccgcccc
     8941 gtcgtagttc gcgtcgccca gcagcaggtc gcagccggga tgctgcgccg catagccgct
     9001 cggatcggtc agagccagaa caaacggatc aatgtcaaag acattaaccg cgccatcgca
     9061 attcaggtcg ccgacctgaa aggcgctcgc ggcgccgggg gcgcacaaca gcaagcccgt
     9121 aacgataatc agtgcatcgc gtctcacgac acgtcctcct atgctgcgcc ggccgcggcc
     9181 ggcaatgatg agaatgcttc aaacctgccg ggggctcagc cacccggcga acctcgcttc
     9241 tttttccctg ccgtcgagct ccggattgcg ttcaagccgc ggggcaggcg ctttgtgttc
     9301 aaagcctaaa ctcccagcgc gcctgcgccc gcgacgatca gaggagaaat taaggcgtca
     9361 ccggctgcac gactggcaag cgccagcttt ccgccaagtg tttttcttgc ggcgagtact
     9421 gctctaccag cccgccgatg gtgtcccgca acaaggcgct catgtcctgc gcattggccg
     9481 gccggccgcc cgcgtccgtg gcggtcttgg ccttgtcggc cggcagcggc ttgaggccgg
     9541 ctttttccgc ctgccggcgt tcggccttga catgggcggc gttgatccgg tcgggtagct
     9601 cggcggcccc gaaccaaatg aggtctttgg tcagatccac gtcgccgggg accaccgcga
     9661 ttacgtaacc cgactggtca ccctggttca gtcgcatggc ggcctggtgg ccgacgtaca
     9721 agacgggctc gccgatctcg cgtggaacga ccaacttcgg gtcgaccttc agcaccagaa
     9781 tcgtgccctt ggtgacctgg aatggctcac tggcccactg atattggaaa ccgtcgttca
     9841 agctgaacgg ccgggcgtag acgacttcgt ccaccgccgc cggcgtattg ggaaccggcg
     9901 gggccgccat tgagatcgaa gtcgcggtca agaggcacac gctcacgagt actgttttca
     9961 tttcttcact ccttctgcaa tgtggcgagc tgcgagcgcc agccatcagt ccatgccggc
    10021 ggtacaggcc ggcgcattga ttgcaggatg ggggggccgg ggtgctcgaa ctcaccccgg
    10081 ccgctgttct cttgaatttg ccactcacct gaggcgcggg gctgggcagc cggccccggt
    10141 ccgcggggcg ggcgtactgt gcgtgaaacc gcacgcccac tagcggcgcc cgcgcccgcg
    10201 gttacgaccg aagctgagcc ttggcgcccg gcaaacgcca agtgtacacc agcctctgct
    10261 acctcgaatg ccacgtacac gttgagtcca catcgaagtc gactggacac aatctctgcg
    10321 cgtgcccggc tggtcgagga caggcgggca tcgccggcgc gcctgcgcgc gcctggttga
    10381 gtctttcacg aatcgtccgg ctcagccaga acaggcttga ccggaggcct tcactaccgc
    10441 tatccgccag gaatccacac tgctttcctg cggagatcgt tgttccgtac gggatgcgag
    10501 gacgtacaca caccgcagcg tgcacatctg ctgcccccaa ccgagggcgc gctcgaacga
    10561 gtcgcgccct ataacccatg cctcgcactc ctaagtcaaa tataacgcgc ctcgccgcgc
    10621 gatccaaacg aaatgtgctc gttcatgctg atttcgtgtg tcggaacaag tagtgcggcg
    10681 gttcaacacg tttcgggctc acaagtcgcc ggtgctttcc gaaaagtgcg gctttgggcc
    10741 cccgatggta tacaaatgcg ctagcaacgc ggccaagcct agtggttgcc acccgttttt
    10801 tcggccagct tcttctcgat cagctcgccc acgatcttgg ggtcggcctt gccgccggag
    10861 agtttcatca cctggccggt cagaaagccg cgggcagcct tgatcttctt gggattagcg
    10921 atggcgtcct ggacggcctg cgcatttgcg gccagcgcct gatccaccca ggcggcgatc
    10981 tggtctgtat cccggctttg cagcaggccc aggcgctctg ccagcgctgt gggcgaatcg
    11041 ctcgagctca gcatggcctc ggcgatctga gctgcggccg tggcgttcac tttgcctgct
    11101 tgaaccaggt tggccaactc ggccaagcgt tgcggcgcga ttccgagccc ggccacggtt
    11161 gtcccgcgct cgttggcatg cttggcccag aagctcagga agtgctttgc cagcgtgact
    11221 ttgtcgccgc cggccgccgc ggcgctgtcc agcagatcgg ccgtccggcg gtcgtcaacg
    11281 agggccggcg cgtcctgggc cggaattcgg tactcggcga cgaagcgctc tgtgcggcga
    11341 atgggcaatt cgggcatttc cgactgtaat tggccgaccc aggcaggatc gaacgtcatc
    11401 gggaccaggt ccggatcagg aaagtagcgg taatcgtggg cctcttcctt ctccctttgc
    11461 ggcaaggtga tctctttgac gtcatcccag ccgcggtttg acttgttgcc catcgcggcc
    11521 gtttcgccgg tttcgcgcca ctgctcgagc tggcggtcga tttcgtattg cacggcgagg
    11581 tagagcgagc ggaacgagtt gaggtttttc acctcgacga tgggcgtgcg aaattcctgg
    11641 ccgccgctga ttatccggag attgatattc ggctcgaagc gcatgtgccc ttcctgcatg
    11701 ttggcttcgc tcacgccgag atagcgaacc aggcgctgaa gctgaacggc gaactcgcgg
    11761 gcttcgtcgg ctgaatgcag atcgggctgg gtcacgattt ccagcagcgg cgttccggcc
    11821 cggttcaggt cgacgcccgt gtagcctgga aagtcgtgga cgttcttgcc cgcgtcctct
    11881 tccagatggg cccggatgat gccgacgcgg cgcggccggc cgtcgcaggt caactcgaaa
    11941 tagccgtccg agctgatcgg caggtcgtat tgggaaatct ggtagttctt gggcaagtcg
    12001 gggtagtagt agttcttgcg gtcccacttg gtgaacgtag cgatccggca attcagcgcc
    12061 agcgcggccc gcacggccag ttccacggcc tggcggttga ttacgggtag cgctcctggc
    12121 aggccctggc agacggggca gacacgcgtg ttgggcggcg cgccgaactc gagcgggcag
    12181 ccacagaaca tcttggtccg cgtggcgagt tgcacgtgga tttccaggcc gatgattggt
    12241 tcaacagtaa gctcggttgc gttggtcata actgaacctg tgagccgcgg tcaccccggc
    12301 catggccgtc gccggcgcag aggtcatgat aaccgcgcgc ccgatgccgc ggcagtcagc
    12361 gccgaggaat tcgctccgcg caccacgcgg caatccgccg gccgcgcgtt gggcggaccg
    12421 agtagcagag cggaccgtct gcggtgagta gcagagccgg ctcgtccagc cgggtttgac
    12481 cggagatccc ccgaagcgct caggtcgcgc gctcagtgcg gatcgagtgc cctcgctggc
    12541 gcatcgggct cggaccgggt gggctgctcg ccgacggcta cttgccggcc gggccggggt
    12601 cgggggggcg acgccgcggg cgggcgcggt gttgcatgag caggctcacg cgatcagaaa
    12661 acaggtcgcc cagttcgcgc gcgatgattt cccgcgcttc cggtagcgcc gtgcgccgtg
    12721 acaggtggca taggacgatt cgctcgttgc gcaactgcgg caggatctcg cgcaggtcgc
    12781 tgacgtggat gtggaagccg gcccgtgccc gctcgaggtg gtccgggtct acgaacgtgc
    12841 actcgagcag caggattttg gcgtcgcgca cgtaatccag cttgaaaaag tcgcccgggg
    12901 ccgtgtcgcc gcagtacgcc accagcggga gctccacgcg gcgcgtgatc gcgacgccct
    12961 ttttcttcag ggccaccagt tcggggccct ccagtccctg gtactcgtcc agcagcttat
    13021 tgcgcacctc gatggccacg taacccaggc tgggatacgc gccgcgcaca cgccggcggc
    13081 aggggtggtt cacttcgaag ggccgcacga tcaggtcgcg ccggacctga atgtcctcgc
    13141 cggcatacgc gggaaagatg ttcgccggcg gttcgttgcc atcgatttcg ccccatacct
    13201 gcagcagccg gcggatcggc tgcaccagcg gctcgggggc atacaggttt cccgggcggt
    13261 tgtcgatgaa catccgctgc gagaagtagt acgccacgcc ggcggcgtga tccatgtggc
    13321 cgtggctgag aaagatgttg tcgacggcca aaacctcgcg gcggccgcgg ccgatgtcga
    13381 agccgatgtt catttcggga ataacgaaga aggtttcctc gccggcggtt gaaaaaccgc
    13441 cgacgtggaa gccgccgacc gcgatttcag ccaaggcgct gggcatgggg gcgagtttat
    13501 aaccggggtg gggtaatttg tcttgcgtaa atccgcagaa attatttgac acacagcgca
    13561 ttgtgttata aacttacgaa gtaacaacat atggtgttca gcctgggcac aaggcggccg
    13621 aagtgcgaca cgggaaccag gcgagcgtga aaatgggaag cttggaccca tgcgttgtcc
    13681 gttctgcaag gccgacgacg acaaagtggt cgactcgcgg ctcacggaag ccggacggtc
    13741 tatccggcga cggcggaagt gtctgcggtg cgggcgacgg ttcaccacgt acgaacgtgt
    13801 cgaggaaacg attcggctga cggttatcaa gcgcgacgca tcgcgcgtgc cgtacgatcg
    13861 cgagaagatg cgcgagggta ttgttcgggc ggcctacaag cggccgattt ctgcggagcg
    13921 catcgaccag atcgtagacg aggtagagga gtacctggtt tctcattttg agaaagaggt
    13981 ctcgagccag accataggcg agcgaatcgc ggcggtgctg cggcgagtgg ataaggtggc
    14041 ttatgtgcga ttcgccagcg tctatcggca gtttgaggac gtgggtgact ttatcgaaga
    14101 ggcgcaggat ttgatcgagc ggtcggccga cgatatcccc ggccaacgcg atctgttcca
    14161 ggaggagcca agggagccgt gaagcccaag aaggtgctgg tcatcacgaa acgcgacggc
    14221 acgctggagt gctttagcgc gtccaaactg cagagctgct tgaggcgcgt gctccaggct
    14281 ggcggcgagg agtggcgaat ggccggcccg ctgagccagg caattgcagt tcatctgcgc
    14341 caactggaag attctgaagt ccccacgagc gaatacgtgt acggctgtgc tcgggccgct
    14401 ttaacgcaaa ccggcctggg ctactcggct gaaattctga aagctcatcg ccggctgcgt
    14461 gaggcacggc gagccagagt gcgagtatat gacccgggcg aatgcgtgcg agcgcccgtt
    14521 cgctggagaa agggggcgat cgttgagacg ttgcgaaacg tctatgacct gggtcagccg
    14581 gtggctcgct atttggccgg tttgatcgaa gagcgtgtgt ttgagttggg ctatcgactt
    14641 ctctcgaagc cgtttatagc ggagttagtt cacaacgaag tattggcctg ggggctcttg
    14701 ttcgagcgga cgtcgcgatc tgcgccgcag gtcgaaagac cgaactccgt tgcgggcagc
    14761 gggcccccca aggaggacta ggatttcgca ggcgggcgct ggcggtcagt gtgtgcgcca
    14821 cggttaagct ggccccggtc cgagtgcgag cgctgatgga tgggagctcg taacgtgctc
    14881 aaggacggca tgtttcgccc atgccgtgcg tgcggcttgg cgaaaccgtt gcgagcttcg
    14941 ttttttcctc gaacctccgt tcccctgatg aagggcgagt cgagtcccgc ccacgcccac
    15001 actcgactcg cccgcccctt cttctgatca gccccgaagc gatgcggaca acgtaattgc
    15061 ttcttctctg gcgccctgtg cttgcggcgc ttgcgttata atccgtgtaa ggactccgct
    15121 gttggccgga tggcagcgct ggtagcgcaa gcggaccggc ccgttttgcg gagcgtcatc
    15181 gtgatgaaac taccggcact tgagaattcg tcctgttacc gcgggctgta cgtgtatgac
    15241 ttcggcgaat ggaccgcggt cggctatacc gccgaggaaa ttgcggtact gctggagagc
    15301 gaggcttatg cccacggcca ggtctaccgc atccagcgca tccagccgga cggctccatg
    15361 gagctacgcg gcgtttctgc tgaacgcttt aagctcgaaa gcggcctatt cttctggcgg
    15421 tccacgcgcg acgaagccga cgccgatttc cgcagcttgc tttcggccgc acagcactcc
    15481 ccgccgccgt gtcgcgccgc cctgcagatg tcagagcggc tggacgcgtg cgatcggtac
    15541 cagtttgtca cggcgctgat cttccccgct gagtacgagg atgatgtcgc cgcctggctg
    15601 ctgcgtttgg gctacgtggg aggcgacgtg gcggaaggcg gcgtgagcca tgtaacgaac
    15661 tactacgagc tggcgcggcg cgtcttggcc gcagagcaac tcacgagcgc tgctcccatt
    15721 cgcccgcgca gtgctgaaga agtcctggcg gcagtccgcc agccggtaca gcgggtcatg
    15781 gtggcctgat gttacgtgtt gttctggcaa agtggctttg gagctgcggc acttggtgcg
    15841 cttcatggcg cagaccgcgc gtgcatcccg agcattttcg ccgccatgtc ttccgtcagc
    15901 agacccgcgg gatggggctg cggatgagtg aatggctgcg aaatcgcgcc cggccgagtt
    15961 ggttgcggat caggcactag gcggggaata cggagtctgg ccgaatagtc ctaccgggtt
    16021 ggacggatgg tggcaacatt cgcattcgcg ctgggggctt acttggtggg agcgatccct
    16081 tttggcctgc tggtcggatt atcgcgtggg gtggacattc gcgagcacgg cagtcgtaat
    16141 atcggcgcca ccaatgccgg tcgcgtactg ggcagacgtt gggggctgct gtgcctggcc
    16201 ctcgacatac taaaggggtt tttgcccgtc ttggcggccg gccggttcct gccgggcggg
    16261 gaagccagtt cgactcgcct gctgcaactg ttagctgtgg ccgtcgcagc cgtgcttggc
    16321 cacatctttc cggtttattt gaagttccgc ggcgggaagg gggtggccac gaccgtcggt
    16381 gtggccctgg ggatctaccc ttattacacg attgccatgc tcgtcgccct ggggtgctac
    16441 gctctgatac gctggacgac cggctgggtt tcgttgggct cgctggcaat cgcggttact
    16501 tttcccgcat ctctgtttgc ttatttgcac tattccggca gacggctgtc ggatttctgg
    16561 ccgctcttgg cggtcgccgt gcttctcgga gcgctcatta tcgttcggca ccggacgaac
    16621 atcgctcggc tgctgcgcgg acaagaggcg cgcgtgacga gcggtgcggc aggcgccgcc
    16681 ggcagtgagc cgcgcgagcg cctgcactaa gccgtcagca gggcgggcgg cgaaccattc
    16741 ggcactacgc tggcctttat cctgcgcgga aatcgaacgt aaagagcaca gacgacccgc
    16801 ttcaaagact ggatgactaa atcgcgtcat ttccgacttc caggccccgc cgaacctttt
    16861 ttaataccga tggcgggcct tgacgtaagc atagatatgg gtattttaag caactaagag
    16921 gtttcacatc cctctggagg aatggccgtg attagtcaaa cgaccgagta cgcactccga
    16981 gccgtagtct ggttggctgc ccatccggat cggccgctga ctgcgcagca gatcgcggag
    17041 gcgacgcgcg ttccggccgg atatctggcg aaggtgctcc agggtctcag ccgcgccggt
    17101 ctgctgcact cgcagcgcgg cctgggcggt ggtttcacgc tggctcgccc gccctcgaac
    17161 gtttcgctgt gggaagtggt gcaggctgtg gatccgatcc gccgaatcac ccaatgccct
    17221 cttgggctcg aactgcacgc gaatcggctg tgtgcattgc atcaggaact ggatcgcgcc
    17281 atcgcgcaag ttgagaagga cttttcacgc tgctccatcg cctcgctgat tgaggagcgc
    17341 ggagaactgg tgccgctgtg tgcggtggct ccgaagggca aaggccgcac gaagacagtc
    17401 gcgaagcgcg ccaagcgcta agggggaagt gagtcagtac agagatcggg gcggggccgt
    17461 ctctgatccc tcggtcgtga agccttgatt ctcttttgcg ccaaactgcg gggctgcgac
    17521 cggccttgcg cttacttacc tctcggccgc ggccgccggt gctggccctt gggtcgccac
    17581 gacgccgctg cctggctagg ggttcgccgc gcgttgccgg ttcctcgtgc agccggctcg
    17641 tgcagggggc ggacgctgga cgccgccacc ttcacttgcg gggactcctt cagttctttt
    17701 atgtgatcgc gcagcgccgc cgcccgctcg aagtccagct cttccgccgc ctggagcatt
    17761 tcctgctcca gttccttgat caattcgtcg cggtcgcgct cttcctcatc ctggtggatc
    17821 acttcgcgca cgcgtcgcat ggccgcgacc tcattcatca aaccggcgcg aatggccttc
    17881 ttgattgtgt ccggcgtaat gttgtgctca gcattgtact tgcgctggat ttcgcgacgg
    17941 cgattggtct cgtcgattgc cttttgcatc gagggcgtca ctttgtcgcc atataagacg
    18001 accagcgcat tcacattgcg cgcgcagcgg ccgatggtct ggatcagcga ggtctcgctg
    18061 cgcaggaacc cctccttgtc cgcgtccatg atcgccacta atgacacctc gggcagatcc
    18121 aagccctcgc gcagcaggtt gacgcccacc agcacgtcga acttgccctc gcgcaactcg
    18181 cgcaggattt caacccgctc caacgtgtcg atctcgctgt gcaaatagcg cccgcgcagg
    18241 cccgcctgct gaatgtagct cgacagatcc tccgccagcc gcttggtcag cgtcgtcacc
    18301 agcacgcgct cgcctgccgc cgcccgctcc tggatccgcc gcagcaaatc cggcacctgc
    18361 ccgcgggccg ccaccacctc gatctccgga tcgaccagcc cggtcggccg gatgatctgt
    18421 tcgaccacct cgccgccgca tttcttcagc tcatacggcc cgggcgtggc gctggtgaag
    18481 aggaccttcg accacatgct ctcgaattcc tcgaagcgca tcggccggtt atccaggcag
    18541 gacggcaagc ggaaaccatg ctcgaccaac acctgcttgc ggttgcggtc cccattgaac
    18601 attgccccga tctgcggcac agtcacgtgc gattcatcca ggaccagcag gaagtccttc
    18661 ggaaagtaat caaccagcgt gtacggcttc gcgccttccg ccgtttggtt caaatgccgc
    18721 gagtaatttt caatgccgct gcagtatccg acctcggcca gcatttccat gtcatacttg
    18781 gtacgcgcca gcagccgctg cgcctcgagc agtttcccct catgccgcag ttgcatcacg
    18841 cgccagtcca gctcttcctg gatggtttgc agggcccgct ccagattgtc ctccggcagg
    18901 acatagtgca ctgccgggta gatgtacacc tggctgcgct cctgcagggc cgccccggtc
    18961 agcgggtgaa tcagcgtgat gcggtcgacc tggtccccga acagctcaat gcggaccgcg
    19021 tactcctcgc cggccggcgc caactcgacc acgtccccgc gtacgcggaa gctgccgcgc
    19081 tgcagttccg tgtgattgcg ttcatattgc agccgcacca gctttttcag cagctcgtcc
    19141 cggtccagct cctgcccgac gcgcacgtcg atgacactgg ccttgtattc gtccggcgag
    19201 ccgaggccga agatgcacga aacgctggcc actaccacga cgtcgcggcg tgagacaacc
    19261 gcgctggtgg ccgccagccg cagccggtcg agatcatcat tccgcgaggc gtccttctcg
    19321 atgtagatat cgcgctgcgg aatgtaggcc tcgggctggt aatagtcgta gtagctgacg
    19381 aagtactcga ccgcgttgtg gggcagcagc tcgcggaatt cctcgtagag ctgggccgcc
    19441 agcgtcttat tgtgcgaaat caccagcgtc ggcagtccca ggcgcgcaat gacatgcgcc
    19501 atcgtgaagg tcttgcccga cccggtcacc ccgagcagcg tctgatactt gcgcccggcc
    19561 tgaaaaccgg ccaccagctt ctcgatggcc tggggctgat cgccggcggg cgcgaacgga
    19621 ctgacgatcc gaaattcgct cattgccgga ttatacgggg ccggctattc ggccgactcg
    19681 ggcgccccgg aagtttcttc cggcttctgc tctccacggc cagcctccgc cccgctgcgc
    19741 gctgcctggt tgaaggccgt caagccgccc agcgccccca gccccatcgt ctccacggcc
    19801 gtcgagggaa cgatgatcat cgaacccttc tgcttcaggc cctcgaacac catgttcatg
    19861 gcccgcaggt gcagcgccac cgggttattg cgataggcct cggacgcgcg ggagaacttt
    19921 tcggcgatct ccgtttcggc cgtgccgagg atgatacgtg attgccgctc gcgctcggcc
    19981 tgggcctgct tgctcatcgc gtcctccagg cccttgggga tgatgatgtc gcggatttcg
    20041 accgactgga ccgtgatgcc ccacggattc gttttggcgt ccagcacctg ctgaatttcg
    20101 tggccgacgc gctcccggtg cgacagcagg tccgccagct cgctgcggcc gattgcgtcg
    20161 cgcagggcgg tctgggccga aagcacgacg gcttgctcaa aatcctccac ctcgagcacc
    20221 gatttctcgg catcccagac catccagaac gcgatggcgt ccacgtttac gggcacggtg
    20281 tccttggtca gcgtctgctc ggcgcgaaaa tcggtcacgc ggatacgctg gtcaacgtat
    20341 tgggcgacct tgtcgatgat cggcagcacg ccgaagacgc ccgagccgcg aatacctacg
    20401 aacttgccga agcgcagcag aacgccacgc tcccattgat aggcgatctg cacgctcggc
    20461 gcaatcaagg ccgcgaccac ggtcgccaac accaaaatcc atggcttgcc gggcgtccca
    20521 cccagtgata acgcggccga cgaggccatc acgcggaaaa tgagataacc acccagcgcc
    20581 accagcgcgc cggtgacgac cacggcctcc caaacctgcg ccgcgcggag cacccctatc
    20641 agtaccagtg cggctaccac ggcataaatc aggggcgcca cgctgaagcc gtagtacagg
    20701 acgtgtacca tcagccctgc cagcattatc acgatcagca ggaacgcatt gaacggcgcg
    20761 aagcggtaat cggcgcgggg cgacgctcca aacgcttcca gcgacacact cggaatgttt
    20821 actttagaca tagcagcagc tcctcaagcg gctctcatcc cgtccgcccg ctgtgaatgc
    20881 cggggcgcag gagagcgggg tcgatcccgc ccagccgttc cagttctttt ccgtgaagcc
    20941 acaacgcgtt ttcatgcgga tcgcgcggat tgaagacaac gcccacccgc tcgcccggct
    21001 gaagcccttc cgtccaggca tacgaatacg tccgatcgcg accggttatc tcgcggcccc
    21061 acgggtcgcg aaactgatag tacacgtcat acgggtgctt cgtgccgaag ttgatggtgc
    21121 caatgcaccg cactcgcacc acggtgccct cggccgctat tccgtaggtc agcagcttcc
    21181 gatcgcgccg ctccgtcatg tacgtcgcga tcagcaagcc cgcgccgagc acaccgatca
    21241 acccgaacat gccgtggagc agcagttgaa cccagggcgg cgccgacccg acgtagcctc
    21301 cggccggccg ggcgcgggtt ggattcgaag ggtcatactc tatttcgatc tggtctccgg
    21361 gctgtattcg gttcccgagg tcctgatcgt aggtgaaacc cgagccggcc accgaagcgc
    21421 cgcccggcaa gtcgtaatgg aagccgattc gccaggggtg ctcagagttc atatgcacgg
    21481 cggtaatgag ctttttcgac gtaaccgtgc ccgtggcgat tgagtgattg gcgttcagca
    21541 acagatcagg taagggcgct gtcagtccgc cgccaatcga cgagtgcaca ataagcatga
    21601 tcagaacgcc agccgggctc atcgccagaa agctgatccc gaggaagaac atgcagcgcg
    21661 ctcgacgcag atagaggcgc gacggcacga cccgcggcgg gtccgggatg acgatggcgg
    21721 tgtgcggccg atccgtcatt tccgcgactc ctccgtgagt tcgcgcaggg cgttggcgac
    21781 ttcgctggtc gtgaacccgt aggccgaagc tctcgacagc agctcggtgc agatctggtg
    21841 cagcatgcgt ttgcgctcca gggctgaaac ctcgacctta gcacttccga taaaagtgcc
    21901 tgtgcccatt tgcgagtcca gcacgccctt gatttcgagt tcctgatacg ctcgcgcgac
    21961 ggtgtttggg ttgatcttca gatccaccga aagctggcga acggtcggca agcggtcgcc
    22021 gcgcgacagg cggccgtcgg cgatggcgaa ctgaatctgg tcgattattt gccggtaaaa
    22081 cggcacgcca ctcttcggat cgatcgcgaa ttggatcatt tgtttgctca tcgcactact
    22141 ttcatggtgt accagtgaaa tagtacacca ggaattcgcg cgcgtcaagt gacaccccgg
    22201 gtaatgaatg cttgtgaggg tcgttaggac gcgaacctca acgccgctgg cggcattccg
    22261 gggtggcacg ccccggctct gctctcgctg ttttggcgcg ccggccttgc gcgctgcttg
    22321 ccgccgcgtg caagtgcggc aatgatcgga cctggagcga gctgatgacg gccaggatac
    22381 tacacggcgc ggcgattgcg gctgagattc gggccgaggt gacgcgcgcc gtcgcgcggt
    22441 ataacccggt cttcgggccg ataaaacttt gtgccatcct cgtcgggacc gacgcacaag
    22501 gccgccttta cgccgattca cagcgaaaac tggccgtcgt ggtgggcgtg cagtacgagc
    22561 tcatcgagct gcccgaaacc gcacgccagg agcaggttca cgcggaaatc gatcgcctta
    22621 acgcggaccc gagcgtaacc ggcatcattc tgcagctacc cctgccgccg cacattgatg
    22681 ccgccgccgc tcaataccac atcgatccct acaaagacgt tgagggcgtg aaccccgcca
    22741 acatcggctg gctgttttac ggcgagccga tcatcgcccc gtgcacggcc ttggcggtga
    22801 acgaaatgat tcgccgcagc ggcgtcgcgg tgcggggggc cgaagccgtc gtcgtcggtc
    22861 agagccggat cgtgggcaaa ccggtcacca tgtttctgct tgaacagatg gccacggtca
    22921 ccggctgcca gatcacgacg caggacttgg cgtcacacac gcgccgcgcc gacatactcg
    22981 tggtcgcggt gggccggccg cagttgatcg gggcagagca cgtcaagccg ggggccgtgg
    23041 tgatcgacgt cggaatcaac cgcatcactg aaacggatgc cgcaggtaag aaagtccggc
    23101 gcacggtggg ggatgttgat ttcgaagctg tcgcgcccat cgcctcggct attacgccgg
    23161 ttcccggcgg cgttggcccg ctgacggtcg cgatgttgct gcgcaacaca gtggaggcgg
    23221 cccggaaaca aagagaacgc gccgggaccg tctgcacccg cgagccgtga taaggtgata
    23281 aagtgatcgg gtgataaggg agatccgcgg acccctttat cactctatca ccttatcact
    23341 ttatcactct ggatcactcc ggcgccgggt cgggcgggtt cggctcgtct tggccgccgg
    23401 gagagttcgt gcgtccctcg actcgcccgt gcaattcatc catcacgctt tccttggcca
    23461 gcggcacgcc agccaggtag gcggcccggc cgagtacgtg ggcggcgacc ggtatcgtga
    23521 gcatcaggaa caccagggcg gtcagggcgc gaacgaccaa ctctaactcg gagaagtaca
    23581 tcgcggccga aaggaggatg cacgccgcgc ccagcgatcc cgccttgctg gccgcctgca
    23641 tgcgcgtata gacgtcggtc aggaccagga ttcccacggc gcccagcagg accagcgcgg
    23701 ttcccgctac ggccagcacg accattagta tttgtaccag aagcgtcacg gttgagcggt
    23761 cctttgaata gcccgggcaa aggcgacggt cgccacaaac gtcaccagcg tcagcacgac
    23821 cgccaaatcc aaaaacaccg gctgcgaata ggcgatgctg gatagcgccg ccgtcaccac
    23881 ggcgatcgtg gcgatcagat caagcgccac cacgcggtcg gccagcgtcg ggccgcgcgc
    23941 caggcgcacg acggcgacga tgatcgagag catggccagc ccaaccagca cctcccagaa
    24001 agtggcccag ccgggcccac tcggggcgac tccctccatt gccaagaggc cagagcagca
    24061 atatagcggt gagctggtcg gcgccccgaa gcgtcggcgc gaggcagtct caaccggcgc
    24121 tcctcggtgt ggcgtcgggg cctcagcgca gcagggccag cacatagcgc tcgaatcctg
    24181 ctttgagttg gcgtcgcact tcctccggat cggtaatgaa catctcgtgt acatacagca
    24241 ccttgcgatc atcagacacg tcgatggcca gagttcccgg cgtgagactg atgaagagag
    24301 ccaggacagt aatctcggcg tcggttttgg catccagggg aacggccacg atgcccggac
    24361 ggcgataggt cttgcgcgta accacgtcgc gggcgacgcg caacgatgag tgcaccagtt
    24421 gccacgcgag aaacccggtg aatctgatta gctgcggcag cttttcgaaa tagcggcttt
    24481 ttcccaggat aggactcatc caccagagca cggcgtaacc gatggcaaag ccgaagatca
    24541 ggttccatcc gccgcggacg gtggccgccc accagatcag ggcgagcaca agattggccg
    24601 cgaacatgat catggcgtgt cctcgcttat ttcgatggtt gagagcccgg agtctgctgc
    24661 gtcgtgcaag ttgacggcgc taatgtaagc cgccgggttg agcaggcggt cggccgcgcg
    24721 gccggctaac gtcagcaggg gggccggaaa aagcccgatc agcacggtca ggccggccag
    24781 cagtgcgatg gggataaagc gcgccgccca ttgaccgggc gccatcggct cggcaggccc
    24841 agatgcgtcc tggggcgcgg atttccaaaa aacctcgttc cagattttca tcatgctgac
    24901 cagggtcagg aggctggtca gcagcgcgac gataactatg gtgttttgtc cggccaggaa
    24961 gccggcttct atcaggccca gcttggccca gaagccggag agcggtggaa tgcccgccag
    25021 gctcagcgct ggaatgagaa acagggccga aagcagcggc gctcgcacca gaccgcccag
    25081 agagcgcaag tcgtatgttc cgccgagttg gcgaactgtc ccactgacca ggaacaggtt
    25141 tgtcttcacg atgatgtggt gaatgatgta gaaaatgccc gcagtcagcc ccagtgtccg
    25201 cacgccggga tcgctgctga ccagcagccc cagtcccatg atcatgtagc cgatctggct
    25261 gatgatgtga aagctgagta tgcggcgcat ctcgaactgc gccaccgctc ccagcactcc
    25321 gacgagcatc gtgagccccg ccaccaccag gatcagtgcg taaatgcccg gaatcgaccc
    25381 aaacaccagt gtgaataccc gtatcagggc atacacgcct accttcgtca gtagcccggc
    25441 gaagacggct gaaaccgcgg ctgggggagt ggcgtagctc gcgggcagcc agaagaacag
    25501 tggaaacacc gcggctttga ggccgaagca gacgccgaag agcatgccgc aggcgcgcac
    25561 caggtgcccg tggcggtcca tcatttccgg aacgcggagc gccaggtcgg ccaggttgag
    25621 cgtgtgcacg gtgccgtaca ggatgcccac cccggtcaga aacaccattg agctgatcag
    25681 gttgatggtc acgtatttga aagccccttc gagttgggca cgctcgctgc ccagcgccag
    25741 cagcaagaag ctcgccatca gcatgacctc gaaccagacg tacagattga agatgtcgcc
    25801 ggtgatgaat gcgccgcaca cgccggccag caatatgtga aataaaggga agtatccgta
    25861 gcgctggcgc gagatgtcta catcggccaa tgagtacact gccaccatca ggccgatgat
    25921 gccggtgatt agcaccatca aggcgctgaa gaggtcaacc gccagcgtga tgccgaaggg
    25981 cgccggccaa ttgccgatct gcacggttcg cacctggccg tcggcagtcg ccgccaagag
    26041 caagccgccg gccgccagca gcgctgccgc gccgactacg ccgcaccagg cccgaaagcg
    26101 cgccgggcgc caaaatgcga ccgtcactcc ggccgtcagc agcggaatca gaatgggcgc
    26161 gaccagcagg agcttcacgt gtcagtgctc ctcagaccgt cggaatcctc agttcccgca
    26221 gcctggtacg tgccgcgcac cagcactgcc gtgaaggccg tcagcgcgaa gctgatcacg
    26281 attgcggtca gaatcaaagc ctgcggcagg gggtcggcat agcccgccgc cggctcggtc
    26341 tgcccggccg gtacgagcgg cggccggccg cgcaccaagc cgccggcggt gaaaatcagc
    26401 aggttggttg catggccaat cagcaccagg cccagtagca cccgcgtaat gctgcgggcc
    26461 agcatgaggt acaagccgcc cgcgaacaat ccgcccagca cgatggccat cagcagcact
    26521 gtcaatcctc cgccagagtt aagagtatga gcagtgccgc gcccaggacc atcaggtaga
    26581 cgccgaggtc gaagaacaga accgatccaa actcgctgcc ttccgggaga gtgacgccgg
    26641 tccacaggcc cgtcaaaaag gggcggccga gaaacagacc ggcacaaccg ctcgccaagg
    26701 ccagcagcag ccccgcgccg atcagcgtaa gcgggtgcac gcgcaaaagg cggcgcgccg
    26761 cac
//
LOCUS       DAOSYS010000014        26451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1506_length_26451_cov_5.554895, whole genome shotgun sequence.
ACCESSION   DAOSYS010000014 DAOSYS010000000
VERSION     DAOSYS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26451
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_26451_cov_5.554895"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="PLU99_01735"
     CDS             complement(<1..1256)
                     /locus_tag="PLU99_01735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15805.1"
                     /translation="MIGLALLTRLTYVFQWQHAPTFSYPIVDEAYLDGWARAIAAGNS
                     FVSGPYFRAPLYPAFLAAIYKVFGPGYVWPRIVQAVLGAASCGLVFLIGREAFGRVVA
                     TLAGLIAATYWTLIFFDGELLAASLSVFLGLLLIWLLLRAGRRPSWKTFGAAGLMLGL
                     AGITRPDVLLMGPAIVVWLAIRFRHQRRSALKFAACVTAGCLAIVLPVTVRNYVAGRD
                     LVLIAWQGGLNFYIGNNPLTDGRTAVAPGMVLDPHGAYDEAIAFAERAEGRPLKPSQV
                     SDYFYGQGWEFIKANPRQALALTLLKARLFWSSWEIANNKNLYFWTERFAPLVRFLPL
                     GFGVIGPLGLLGMTLCWRRRLELFPLWGSILVYMAAVVPFFSAARFRLPIVPVLIVLG
                     AWAAVAGYRALRERQWRSLLAGATVL"
     gene            complement(1656..2855)
                     /locus_tag="PLU99_01740"
     CDS             complement(1656..2855)
                     /locus_tag="PLU99_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO15806.1"
                     /translation="MARLRTKAFTLIELLVVVAIIALLISILLPSLQAAREQAKRVVC
                     SGNMSQIAKGSLAYSVDDWREQIIPLSVGVVTDLHGKGFSNSSWGWRAAANFSFGGRT
                     PTRPMLHNGQSIVAMTDYSQRTGGDGSFGGQMGDPSKNLWGAMHRPLNRYIYGEMTAN
                     DRFKVPMYQDPADVGYAYSRQDASSTGDEGEHQFDAPPDSADIPCYDYLGNSYRVNTA
                     GVVWGAQGAAGGEGASFPSLRGSLNVGVEGHAASTVENTSRVPMYCDPLFYWWSRQDP
                     NAPPIDEKMLIGWHKKIMSDNVVFCDGSARQTKVETLDEFDVDTLNKMNFSKDPQDQQ
                     NPWSFLRRGRTWQTDTYPAPAALIKTYDINDLKRCLFDASQMQYLASKKGWPFDSYTV
                     NRPPGER"
     gene            3162..3779
                     /locus_tag="PLU99_01745"
     CDS             3162..3779
                     /locus_tag="PLU99_01745"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOO15807.1"
                     /translation="MPASEAELVKQAVMGNTRALTALLERVGPEVRRRIAPAIPSRWR
                     PMVSLEDLMQETYIDAFLDVHRFHMRGDNSFLSWLMTIARRNLVDALRMLEADKRGQG
                     ARQVAPNAEDLTALEERLAWTRTTPSRHVARSENRRLLERALEQLPEIYRTVISLYDL
                     QGKSVKEVAAVLQRSPGAVFMLRSRAHRLLSANLGTASLYLSDTV"
     gene            3810..5402
                     /locus_tag="PLU99_01750"
     CDS             3810..5402
                     /locus_tag="PLU99_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOO15808.1"
                     /translation="MAGAGHRKGTEHEREIIRTAQIRAQELKRAWLASAPAVDCIPGY
                     TITREIGRGGMAVIYEAQQKEPPRAVALKVMRGGATVSKHRLRLFRQEMETLARLSCP
                     NIAAIYGAGTTNDGRHYFAMELVRGSPVTEYVHLQNLSLRQILELFQQICQAVHYAHQ
                     RGVIHRDLKPSNIMVDGDGNPKVLDFGLARMVETDDAVRAMMSESGKIMGTLPYMSPE
                     QAEGDQDGLDIRTDVYSLGVILYELLTDRLPYDVPPTDPLQVRQAIIEQPPCRPSRYR
                     RSVRGELELIVLKALEKEPAKRYQSALALGEDLGRFLRGQAISARTPTIPYLVRKFIA
                     RQKLPVALTTVLLATLAGFAGWERIQANQRRLDEQQRQQSLERWQQKTRAEMRDLLRI
                     GQFWEAVVMEVAGRDVLDEAAERIDGTFVDSAAPAATVREILARRYAALGETERAARE
                     RGLAAAALRKSARQRIEHRDWAAAEALLLREYDIRRKFDEAGARETARLLADVYGASG
                     RPQRKEEWLNRADAPLSTDGNQ"
     gene            complement(5457..6836)
                     /locus_tag="PLU99_01755"
     CDS             complement(5457..6836)
                     /locus_tag="PLU99_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOO15809.1"
                     /translation="MKTIIEPFRIKVVEPIRMTTREQRERMLVDAGFNLFLIKAEDVL
                     IDLLTDSGTGAMSSLQWAGIMRGDESYAGAKSWYVLKDKIQEITGFPHILPTHQGRAS
                     ERILFELVGGPGKIVPNNSHFDTTRANIEHAGAQALDLVIDEGKQPATRLPFKGNIDL
                     RKLENLIQEVGGGRSGTALRERIPLVMVTVTNNSGGGQPVSLENLRGVRKLCDRYGLP
                     FFLDACRFAENAWFIKQRELGQQDRSVREIVHEMFSLADGATISAKKDGLVNIGGLLL
                     LRDDELARRADELLILTEGFITYGGMAGRDLEAMAQGFEEVVDEDYLQYRIRSTEYLG
                     EKLLAAGVQIVEPPGGHAIYIDAKRFCPHIPPAQFPGQALVVALYRHAGIRAVEIGSV
                     MFGQNGTPPPMELVRLAIPRRVYTQSHIDYVIEAIIEVYQQRDRLRGLEIAWEPPSLR
                     HFTARFREV"
     gene            complement(6872..8281)
                     /locus_tag="PLU99_01760"
     CDS             complement(6872..8281)
                     /locus_tag="PLU99_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15810.1"
                     /translation="MVVLALAIVLSLIAGWRAWQFWNGLSGVRLARLTWLDYERAQRA
                     ADARMSRAARQGLIAANVSHLFTSALLDPDVYPARRETLQRMLAELTDTQIGRVQLAA
                     FLMLEMERAAAPVEQWWPRLAPVLIGLERAQFRHYLAPLEDSRTEMYESLGLRPGAAR
                     AQAEYTIGQPHGPLLQYLAPRLDTLARTRSAAEDGSDVVCRRVLLRLLQQWVLDDGPP
                     GMRLLAADLLAQAISDAGEEAQDPGKQSFAPLPAPAAQIAAQLRQWRAAYRTAAAEHP
                     PLPYPLGSGDVPDLRPDSSGRFASCLQLRMWMLGALCAAALVNVVALVLARRAFLTAP
                     KRPNQLLVLGIVAAFGLGWLAWMTLGDASLADDVRRLAPSTPGLPRTPIIAFGLLALL
                     LLSSALLPPRVGAPRRARAARTAVLAIWAMLILSSCSLLFLDQTPGRIDFYPPGDRVT
                     LLIGTSYAQVFDALQAWQP"
     gene            8902..11154
                     /locus_tag="PLU99_01765"
     CDS             8902..11154
                     /locus_tag="PLU99_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOO15811.1"
                     /translation="MSFVQYHYGDESALPWSDARWTFYDFHYTPSTVFDGADVMIGSL
                     LDIELQYNLYRVNHLQPDRAAPTDVTLTIRGEPVSGQTYQAIVDVGIEPDGVPRTLRI
                     YIVQVLDHWPFTKPYYRNGFKQAAPTVDVSLNPGETQTLQATFTFDAESWANREDIKL
                     IAWAQLPLDTAPAVVWQSATRQWPLVSYPGDWDGDGVADTADNCPRRYNPDQTDSDGD
                     GVGDICDKCLNQPDPAQDDPDEDGIGSACDNCPVLHSLNQYDTDGDGVGDPCDSCPEV
                     PAPAGVDQFGRLRGSMDIDCDVDADDLALFGPCIGGPGVTIAPDGCTPEDFARADLDG
                     DGDVDLSDQAIFARNFSGSLPSPDMFAGYQSCIGCHAEQADLWIHTIHRTAYATVADS
                     PDILICLPCHTVGYGKRSGFVDMETTPHLAGVQCESCHGPGSNHNLDPIAYPLTVSYG
                     TALCGECHQSCHGVCGDNSHPQLEQWSRSKHARALPDMKADEQAWDDCLRCHSTDYRL
                     KPDGEKPGLYEAQYSIECIACHNPHGSPNKGQLRLPPSLICIDCHHMEGESGYWDLPA
                     QPQAQILHGQLGFYLDETPMEGPYSMHWWGIADECVVCHMHTEPRTPEHEADSGHLFT
                     SNVRACMPCHSEEVARQLVDELHYEVSTRSSLIARHFDPFDPLYVDPTWLPPDEVIAW
                     IISYYNNQLVIADRSFGSHNPAYIRALLAEAEAYLGIEPWPALRAAGRDDLLWSFGVH
                     VPQPKKAEVP"
     gene            11154..12188
                     /locus_tag="PLU99_01770"
     CDS             11154..12188
                     /locus_tag="PLU99_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOO15812.1"
                     /translation="MTITRRDFLKLAAAATAAGHLSTVAFAKLQKVLRAPGGPRVVWL
                     QGAGCDGCAVSFLNSIHFAGVDDLLLNTLDVRFQNNLMAATGELAVSAATGVLPEPGY
                     ILIVEGSIPTAAGGRYSYLWPEMTMLDGVISFAANAAYIIAIGACACYGGVTKGAPNP
                     TGAQGVGDVIGADSRLINLPGCPMHPDWLVSTVTRLLTTGRVPPLDAHRRPLEFFGRL
                     LHSDCPRRHKHCGEIQLASQLGEPGCLEFLGCKGKHTNAECPMRKWNSSGPGESGVNW
                     CIGAGSPCIGCVNPNFPDGMSPFYQYLPPPRGLIERGDEEPAETPAPAHTGQAPASDA
                     KKTRSAGGTR"
     gene            12188..13627
                     /locus_tag="PLU99_01775"
     CDS             12188..13627
                     /locus_tag="PLU99_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOO15813.1"
                     /translation="MPTTFTIDPVTRIEGHLEVQITVDLVGGEQKVVDARSAGTMFRG
                     FEMILPGRDPRDAALYTQRICGVCPVPHALAACLALDQAFGVTPPDNGRIMRNLVLGA
                     NFIDSHLLHFYHLSALDFIDTTGVLDMPPWTPRYVTADMLTGPAAAALVAHYRTALAI
                     RRKAHQMGAIFGGKLPCVSSFVVGGCTDQVTAKKIQDFRALLAEIRAFIDDVYLPDAA
                     ALAAAFPSYYSLGAGPGNLLAFGVFDLDASGATKLLGPGRYTKGVPGTFNAAQITEDI
                     TYGRYNGADDLNPAVGVTQPNPDKPGAYTWLKAPRYSNQPYELGPLARMWVNGRYTNG
                     ISAMDRIMARAAETKFVADAMDGWLDQLEPGQPAYTPSEIAASATSAGLTEAPRGGLG
                     HWMDVTNHVISRYQVVTPTSWNAAPKDHQNQRGPMEEALLGVPVPEITKPVEVLRIIH
                     SFDPCLACAVHLLRPGEKAGTVVRVATWS"
     gene            13663..14250
                     /locus_tag="PLU99_01780"
     CDS             13663..14250
                     /locus_tag="PLU99_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HOO15814.1"
                     /translation="MTDNAEQGRAPVLVLGIGNILLRDEGIGPRVAQLLQAAALPAGV
                     EVCDGGTAGFNLIDVLAGRRKVIVIDALDAGHAPGSIVRLTPQDLTRQDRPNLSLHDL
                     GLLEALDMAQQLGEAPQEVVIFGIQPQDLSCGLDLSPTIRACVPELVNLVLAELKGEN
                     GRQDGGQAPPQSVVFSEVQEEVKSGEEAQERAGVA"
     gene            14247..14999
                     /locus_tag="PLU99_01785"
     CDS             14247..14999
                     /locus_tag="PLU99_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HOO15815.1"
                     /translation="MKYSLLLPAAVLLVLTQPAWAEPAWGSNCLACHGVLQPGLIAIM
                     GEDGTADPDESVTGAPDRGLLPVFQVYPGNSKSLQAALVGLNEGDRYSVELKRLRFSG
                     VETGSTLLFSPDCDWPEWGDSPYYTQPEVGYCWGEGPTTFTYTIGTDGEGAFDYFDLV
                     FAVAGKFADTGELFYAEEHFYLQLTWLRGDINCDGSVNVFDIDPFVQALTDPAGFLGA
                     LPGCTIENADINRDGSVDVFDIDPFVLLLTGG"
     gene            15251..16270
                     /locus_tag="PLU99_01790"
     CDS             15251..16270
                     /locus_tag="PLU99_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15816.1"
                     /translation="MEENAAVTVQPSPVGAAPDRLSAYLRLKRVFLRTLVISLTVCAL
                     VAVGALLLGTFNETTAKVLTTLGALAVHSGAAMSCASTLERRRWPKLSAVGLILFAAS
                     FCVLITCIWWPGWFDESTIRAILTFGTLAGFYILAIPSADLWERRTRRVLAGAGLLVC
                     AAAFLMVLACIWATDSRNVEFAKATGIASVIAFSVAHTALLVRVPAGRGADWLLMVAL
                     GCVWLLAAMASASIAWEIDEEFWYRWLGAVGVLDACSSFALLIMAKLRQVGKVENLQS
                     AAARVELRCPRCTALQTVDAGVAKCSACGLKFRIEIEEPRCAKCDYLLWQLPERRCPE
                     CGTEF"
     gene            16609..17520
                     /gene="rpsB"
                     /locus_tag="PLU99_01795"
     CDS             16609..17520
                     /gene="rpsB"
                     /locus_tag="PLU99_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOO15817.1"
                     /translation="MASALVRELLDAGIHFGHQCSRWNPKMKPYIYGKRNGIHIIDIR
                     ETLRGLLRARKFLTQLVAGGEDVLFVGTKRQAREVVEKEAGRCNMHYVSERWLGGTLT
                     NFRTIRGRLQRLEELESLVASPVWETDYSKKMKSTLSRELKKIQRNLSGIRKMNRLPG
                     ALVLVDVRKEHNAVREAQALGIPTIALLDTDSDPEMTNIPIPGNDDSMRSIALVLRYL
                     ADAVEEGFKARPEPRPQAEQAEGSEFGAPPPRRRGRRPMPMGRGGPGGPEPAGSAVAT
                     TEEESPAAPEGEPAHDFGVQAQGNAPA"
     gene            17601..18428
                     /gene="tsf"
                     /locus_tag="PLU99_01800"
     CDS             17601..18428
                     /gene="tsf"
                     /locus_tag="PLU99_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOO15818.1"
                     /translation="MEITPAMVMQLRNETGLPMMECKQALIASQGDKEKAIEWLRRKG
                     LGQVTKRAARETTEGRIAVHVDPKGGRAAIVELLCETAPVANTKDFIDLAAQIARHAV
                     QLDAPTPESVLDQPLLDDAGRKLQDYMHDAINRIRENIRIAHVGTLTGHIGHYLHHNA
                     QVGVLVEMSAEAPDALKSDVCMQIAAMRPVCARREQVPAELVETERRLATEQAQGKPA
                     NIIEKIVEGKLNRWYAEMVLVEQPFVKDDKKTVGQVLREAVPNLTVNRFLRFEVGQE"
     gene            19102..20913
                     /locus_tag="PLU99_01805"
     CDS             19102..20913
                     /locus_tag="PLU99_01805"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOO15819.1"
                     /translation="MSQAQASLAPASVTGCGPECDFCWLDTEFENNCPEDWYGTGNGC
                     DCGCQWVDPDCDTSPIGACCSPIQGTQCIDGLDYLDCWDFGGAYLEGVTCAETDCIRC
                     PGCDRCWTATMFEGGCPAEWEGNGPCDCGCQFEDTEDCQGSWSAGACCSPFQGECFDG
                     LTSDECRTLEIGVYVEGQTCAETDCVRCAPECHLCWEGTIMEGDCDPAWEGNGLCDCG
                     CQFEDTLDCFGPPPTGACCLPLQGGQCIDNLTFEECWNMGYAPLEGLTCAETDCVRCG
                     PECDWCWIGTISEGNCDPSWANDGDCDCGCQWVDPDCPGGACCRGDTGACRVTVEADC
                     TGPGETYWGDGTSCSPNPCPQPLPTGACCDSSAACTVSTEAECAGVYQGDGTNCTTTL
                     CPPPTGACCYVAGVCSITTEVGCLGTYWGDGTDCSPNPCPGPTGACCAITGECTISTK
                     AECDGTYWGNWTDCSPNPCARTGACCDGTACTISTQADCRGDYLGDATVCTPNPCAYW
                     YRDADGDGHGDLNQAVRAAQRPAGYVEASDDCDDANPDIYPGAVENCNELDDDCDGQV
                     DEGACDSERAAPGACAPASALLLTATLIGLARGRRRR"
     gene            complement(20916..23186)
                     /locus_tag="PLU99_01810"
     CDS             complement(20916..23186)
                     /locus_tag="PLU99_01810"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOO15820.1"
                     /translation="MQSAKLPRTGLVCLMLGAMLAGCPRNGERDPGDGEQARSITVTQ
                     PADGAAKPTYPGGSTLAIRWSSTGAIANVRVELLYDASASGSPDRSLLTTIADLTEND
                     GAYDWVVPVSYRVFPGQCVRVSDADDAGVFGLSCEITVGDDLLTPDAPTQPSPANGAV
                     DIPVELKLDWSDAARATSYDVYFGTTNPPPLAGNTVTSDWPLAKLSHGTAYYWSVVAT
                     NSAGSTPGPIWSFTTLPDKTEPSPPEPPIKLPDTPGAPSPADGAGDVSVESDLYWEVA
                     AGATSYDVYFGTDNPPPLMGNTSASKWTLATLSYSTTYYWNVVSKNSAGTAAGPIWSF
                     TTGPPPLVAPDEPSGPIPPDGAGDQPLDSWLDWDDAAHAESYDVYFGTSDPPPFVGNT
                     VFSDWTPAKLQYSTTYYWSVIAGNGVGQTPGPTWSFTTQAATAGEMVFIPAGEFQMGD
                     AFGEGTSAELPVHTVYVDGFYMDVCEVTNQQYATALNWALAQGGLISVSGGMVYQAGG
                     GHPYCHTNTSWSISRITWDGNEFGVVAGKEHHPMAAVSWYGAAAYCNWRSAMEGLGVC
                     YNLSTWECDFDAAGYRLPTEAEWERAARGGAAGHRYPWSDQDTVQQARANYWSTASFL
                     YDTNPTSGDSPLWGAGPEPYTSPVGFFTGALQLRTDWNWPGAADTYQTSNGVNGYGLC
                     DMAGNVWEWVNDWYHSAYYEISPYDNPRGPESGTWRSMRGGSWDDSAFGCRVANRGKN
                     EPDDPDIDDGFRCVRK"
     gene            complement(23511..24215)
                     /locus_tag="PLU99_01815"
     CDS             complement(23511..24215)
                     /locus_tag="PLU99_01815"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOO15821.1"
                     /translation="MSIPYVPGEPLSCEQIRQLDALAIEQIGVPGIVLMENAGRGIAE
                     FVYGCLVNPAADRVLILCGAGNNGGDGLVVARHLRNAGVPVTVALSVPPEKLKGDAAT
                     NMRIVEHMDIPRLHAFEAAGLDKVRAEAQHAAIIVDALLGTGSRGAPRGVMAELVEIA
                     NAAPRARRIAVDIPSGLDGDTGQASQPCFRADATVTMVAAKAGFSAPSAAAYVGRVVI
                     VGIGAPRTVIAPGRVQ"
     gene            complement(24242..24997)
                     /gene="sdhB"
                     /locus_tag="PLU99_01820"
     CDS             complement(24242..24997)
                     /gene="sdhB"
                     /locus_tag="PLU99_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOO15822.1"
                     /translation="MLEKFKVRVQRQDGPATKPYTQTFELPYKPDMNVISVLQEIAAH
                     PVTADGQHTTPVVWDSNCLEEVCGACSMVINGFVRQACSALVDELLRAGPVIDLKPMS
                     KFPVIRDLFTDRSRMFDALKKVRAWIPVDSYAPLGPGPIVPPEVNERRYELSRCMTCG
                     CCLEACPQINPHSDFIGPQVIGQVVLFNMHPTGAMTASYRNEAMMGPGGITGCGNAEN
                     CVKVCPKDVPLTWAIGKLGRDTTTHAFKRWLMR"
     assembly_gap    24998..25097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25098..>26451)
                     /locus_tag="PLU99_01825"
     CDS             complement(<25098..>26451)
                     /locus_tag="PLU99_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO15823.1"
                     /translation="GGPGLVYGKSTMSMVCTGTANARAYKAGAIYANGEFVQVHPSAI
                     PGADKLRLISEAARGEAGRVWVPRKQGDRRHPNEIPETERWYFLEEKYPTYGNLLPRD
                     IATREIFQICRDGYSVSPHTYAVYLDLTERATGVKREVLEHKLGAILDMYRKYKGVDP
                     LSEPMQIFPAVHYSMGGLWVDYERDDRTGGLRVDSPRNQQTNVPGLFAVGECDYQYHG
                     ANRLGANALLSCIFAGLLVGRCMRSFLAALPGGSVDKVSTSLFEGEVARNKQEYEQLL
                     AREGRENAYLIHRELGELMTRNVTVIRHNADLRRTLDKLEKLAERYSHAPVADQGGLW
                     TNQSLSFTRAVGDMILLARVITECALLRDECRGAHYKPEFALPAPTAERPAELRKEAE
                     EWCRRFKEQNDRWLKTTLATYTLDGPRITYEPVDLYSIPPRPRTYGMKGAEIVERVWA
                     E"
BASE COUNT         4818 a         8590 c         8217 g         4726 t
ORIGIN      
        1 cacagaaccg tcgccccagc caggagcgat cgccattgcc gctcgcgcag cgctcggtag
       61 cctgctacag ccgcccacgc accgagcacg atcagcaccg gcacaatcgg caaccggaac
      121 cgggccgcgc tgaaaaatgg cacgacggcc gccatgtata ccagtatcga gccccacagc
      181 ggaaacagct ccaagcgccg ccgccagcat agcgtcattc ccagcagccc cagcggtccg
      241 atcacgccga acccgagcgg caggaacctg accagcggcg caaaacgctc ggtccagaag
      301 tataagttct tgttgttcgc aatctcccat gagctccaga acagccgcgc tttaagcaac
      361 gtgagagcca gcgcctgtcg cggattggct tttatgaatt cccacccctg cccgtaaaag
      421 tagtccgaaa cctgcgatgg cttcagcggc cgcccttccg cccgttccgc aaacgcgatc
      481 gcctcgtcgt aggcgccgtg cgggtcaagc accatgcccg gcgcaacggc ggtccgtcca
      541 tcggtgagcg gattattacc gatgtagaag ttcaagccgc cctgccaggc gattagcacc
      601 agatcgcgcc ccgccacgta gttgcggacg gtcaccggca gtacgatcgc caaacacccc
      661 gccgtcacac aagcggcaaa tttcaacgcg ctgcggcgct ggtgccgaaa gcgtatcgcc
      721 agccacacca caatcgccgg ccccattagc agcacgtccg ggcgcgtgat ccccgccagc
      781 cccagcatca gcccagcagc cccaaacgtc ttccaacttg gccgccgccc cgctcgcagc
      841 agcagccata tcagcagcag ccccagaaaa acgctgagcg acgccgccag cagttcgccg
      901 tcaaagaaga tcagcgtcca gtaggtcgcg gcgatcagcc ccgccaacgt cgctacaact
      961 cgcccgaacg cctcccgtcc aatcaagaaa accagcccgc acgaggccgc ccccagcacc
     1021 gcctgcacga tccgcggcca cacgtagccc ggcccgaaca ccttgtagat agccgccaga
     1081 aaggcgggat aaagcggtgc ccgaaaataa ggcccgctga cgaaactgtt gccggccgct
     1141 atggcccgcg cccaaccatc caggtacgcc tcatcaacga tcgggtatga aaaggttggg
     1201 gcgtgctgcc actggaaaac ataggtcagc cgcgtcagca gcgccaagcc gatcaagccg
     1261 ccgagcagtg gcccgtcgat tcgccaactg gcgtgctttg aaacgccgtc cgtcgcgagt
     1321 gccggaggct cccgcttggg ctgaacgcgc gtgctccgcc cctcccgcct ggtcttaatg
     1381 gcgcgaattc gtctctcctc aactcacctg gcgccggtca gtttagccgc cctacgtggg
     1441 ggaaacacct gccgcgcggc ggaaatcctg aatgcggcaa gcagggcggc ggcacctggc
     1501 ggacgcaccc cctcgtcgat gcaggcggcg gaattggcgc gaaccgctcg aagtggcgcg
     1561 agcaggagca gccatccacc gggaggttgc gcaaagaaaa gcgccgcgcg agggttagca
     1621 tcgcgcggcg cttcaacttt cagtttagcc ggttcttatc tttcgcccgg cggcctgttc
     1681 acggtgtagg aatcgaaggg ccagcccttc ttcgaggcga ggtactgcat ttgagacgca
     1741 tcaaacaagc atctcttcag gtcgttaatg tcgtacgtct tgatgagagc cgccggcgcc
     1801 gggtatgtat cggtttgcca ggtacggccg cggcgtagga agctccaggg attctgctgg
     1861 tcttgcgggt ctttgctgaa gttcatcttg ttgagcgtgt cgacgtcgaa ttcatcgagc
     1921 gtttcaacct ttgtttgacg cgccgacccg tcgcagaaga cgacgttgtc agacatgatc
     1981 ttcttgtgcc agccaatcag catcttttcg tcgatcggcg gcgcgtttgg atcctgccgg
     2041 ctccaccagt agaagagtgg atcgcagtac attggtacac gagaggtatt ctcgacggtg
     2101 gaggcggcgt gtccttcgac gccgacgttc agggagcccc ggagcgacgg gaagctcgcc
     2161 ccttcgccgc cggcagcgcc ttgggcgccc cagaccacac cggccgtatt cacgcgatag
     2221 ctgttgccca aatagtcgta gcagggaata tcagcggaat caggcggggc gtcgaattgg
     2281 tgctcgcctt catcgccggt actcgacgcg tcctgacgcg aataggcgta gccgacatcg
     2341 gccgggtcct ggtacatcgg cactttgaac cggtcgttgg ctgtcatttc gccgtagatg
     2401 taacggttga gcgggcggtg catcgcaccc cagaggttct tggagggatc gcccatttgg
     2461 ccgccaaacg aaccgtcgcc gccagtccgc tggctgtagt ccgtcatggc aacgatgctt
     2521 tggccattat gcaacatggg gcgcgtcgga gttcggccgc cgaaggagaa gttcgcggca
     2581 gcgcgccacc cccagcttga attgctgaag cctttgccgt gcaggtctgt gacaacgccg
     2641 accgagagcg ggataatctg ctcgcgccag tcatcgacgc tgtacgccaa agaccctttc
     2701 gctatttgcg acatgttgcc gctgcaaacc actcgttttg cttgctcgcg tgcggcctgc
     2761 aaactgggta agaggataga aatgagcagc gcgatgatcg ccacgaccac cagcagctca
     2821 atcaacgtga acgctttcgt gcgtaacctg gccatctctt tctcctttga agacaaggag
     2881 ttgcgcacgg tccgttccgg cgcagctcgc ccgagaaacc tgccacaccg cactgcgcgc
     2941 cgcgtgcgca cggccggggg caggcgtctc gtcacacttc cttcatccat aaggagcgta
     3001 aagcgggcct tgtagtaacg caagaggcga atttttgttg cgttactcct ctgaagaaga
     3061 ccgcagaggt cctataacac caagacaacc cccttgtaga gagcattggt ggttgatacg
     3121 ctgatgttga cgcaccacac gcatggagtt gagagccacc cgtgcccgct tccgaagccg
     3181 aactagtcaa acaagctgtt atgggcaata cgcgtgcgtt aacagcactt ttggagcggg
     3241 ttggccctga ggtacgccgc agaatcgcgc ccgccattcc ctctcgctgg cggcctatgg
     3301 tctctttaga agacttgatg caagaaacct acatcgacgc gtttctggac gtccacaggt
     3361 ttcacatgag gggcgataac tcatttctgt cttggttaat gacgattgcc aggcggaatc
     3421 tggttgacgc cttaagaatg ctcgaggccg ataagcgcgg gcagggcgct cggcaggtgg
     3481 ccccaaacgc cgaggacctc actgctttag aggagcgcct ggcttggacg cgcaccacac
     3541 ccagccggca tgtcgcccgg agcgagaatc gccggcttct ggagcgcgcc ctggagcaac
     3601 tgcccgaaat ttatcgcacg gtcatctccc tgtacgatct ccagggaaaa tccgtcaaag
     3661 aagtcgcggc ggtgctccaa cgcagtccgg gcgcagtctt catgttgcgg agccgtgctc
     3721 atcggctgct ttccgcgaac ctcggcaccg catcgctcta cctcagtgat acagtgtgat
     3781 ctgcagtggg tgctaagaga cgatccctga tggccggcgc cggtcaccga aagggcaccg
     3841 agcacgagcg tgaaatcata cgcacggctc aaatcagggc ccaggagctg aagcgagcct
     3901 ggttagcgag cgcgccggct gtcgattgca ttcccggcta cacgattacg cgtgagattg
     3961 gccgcggcgg aatggccgtc atctatgagg ctcaacagaa ggagccgccg cgagccgtcg
     4021 ccctgaaggt catgcggggc ggggcaacgg tcagtaagca ccggctacgg cttttccgcc
     4081 aggagatgga aacgctggcg cggctgagtt gtccgaacat tgcggcaatc tacggggcgg
     4141 gcactacaaa tgacgggcgc cactacttcg cgatggagct ggttcgcggc tcaccggtga
     4201 cggagtatgt gcaccttcag aacctctcat tgcggcaaat cctggagctc ttccagcaaa
     4261 tctgccaggc agtgcactac gcccaccagc gcggcgttat tcatcgcgac ctgaagccct
     4321 cgaacattat ggtggacggg gacggaaacc cgaaggtgct ggacttcggt ctggcgcgca
     4381 tggtcgagac ggacgatgcc gtgcgggcca tgatgagcga atccggaaag atcatgggca
     4441 cgttgcccta tatgagtcct gagcaagctg aaggcgatca ggacggcctg gatattagaa
     4501 ccgacgtcta ttcgctgggc gtgatcctgt atgagttgtt gacggaccgc ctgccgtacg
     4561 atgtccctcc tacagacccg ctgcaagtgc gccaggcgat tatcgagcaa ccgccctgcc
     4621 ggcccagccg atatcggcga agtgtgcggg gcgagcttga gctgatcgtg ctcaaagctc
     4681 tggagaagga accggccaaa cgctatcaga gcgccctcgc cttgggtgag gacctgggcc
     4741 ggtttctgcg ggggcaggca attagcgcgc gcacgccgac cattccctac ctcgtacgca
     4801 aattcatagc gcgccaaaaa ctgccggtcg cgctgactac tgttctcctg gcgacgctgg
     4861 cgggtttcgc cggctgggaa cgcatccagg ccaaccagcg gcggctggac gagcagcagc
     4921 gccagcaaag cctggaacgc tggcaacaga agacccgcgc tgaaatgcgc gacctcttgc
     4981 gcatcggcca attctgggaa gccgtggtta tggaagtagc cgggcgggac gtgctcgacg
     5041 aggcggccga gcgaatcgac ggcaccttcg ttgatagtgc cgccccggcg gccacggtgc
     5101 gcgagatcct ggcccggcgc tacgcagctc tgggagaaac cgagcgtgcg gcgcgcgagc
     5161 ggggattggc cgcggccgcg ctgcgcaagt ctgcccggca gcgcatcgag caccgcgatt
     5221 gggcagcggc cgaagcgctt ttgctccggg aatatgacat aaggcgtaag ttcgatgaag
     5281 ccggcgcacg cgagaccgcc aggctgctgg ccgacgtcta cggcgcctcg ggcaggccgc
     5341 aacggaaaga agagtggcta aaccgggcag atgctcctct gagtacggac ggcaatcagt
     5401 gacttcgccc gcggagcgtt gcggcaaaca gcgggtttct cagcacaaat cttcgttcat
     5461 acttcccgga aacgtgccgt gaaatgccgc agtgatggcg gctcccaggc gatctccagg
     5521 ccgcgcaaac ggtcgcgctg ctggtagact tcgatgatcg cctcgatcac gtagtctatg
     5581 tggctctgcg tgtaaacgcg gcgcggaatc gccagacgca ccagctccat cggcggcggc
     5641 gtgccgtttt gtccgaacat cacgctgccg atttccacgg ctcggatacc ggcgtggcga
     5701 tacaaggcca ccaccagcgc ctgcccgggg aactgggccg gcggtatgtg cggacagaaa
     5761 cgcttagcgt caatgtagat ggcgtgcccg ccgggcggct caacaatctg cactccggct
     5821 gccagcagct tttcacccag atattcggtg ctgcgaatgc ggtattggag gtaatcctcg
     5881 tccaccacct cttcgaagcc ctgcgccatg gcctccaggt cgcgccctgc catgccgccg
     5941 tacgtgatga acccctccgt tagaatcagc aactcatcgg cccgccgcgc cagttcgtcg
     6001 tcgcgcagca gcaacagccc gccgatgttc accaggccgt ctttcttggc ggagatcgtg
     6061 gcaccgtcag ccaggctgaa catttcatgc acgatttcgc gcacgctgcg atcctgctgc
     6121 ccgagctcgc gctgcttgat gaaccaggcg ttctcagcaa aacggcaggc gtccaggaag
     6181 aacggcaggc cgtagcgatc acacagcttg cgcacgccgc ggaggttctc aagactcacc
     6241 ggctgcccgc cgccgctgtt gttggtgacc gtaaccatga cgagcgggat gcgctcgcgc
     6301 aacgccgtcc cgctgcgtcc gccgccgacc tcctggatga ggttttcgag cttgcgcagg
     6361 tcgatgttgc ccttgaacgg caggcgcgtc gcgggctgct tgccttcgtc aatgaccagg
     6421 tcgagcgcct gcgcgccggc atgctcgatg ttggcgcgag tggtatcaaa atggctgttg
     6481 ttcggcacga ttttgcccgg gccgccgacc aattcgaaga gaatgcgctc gctggcgcgg
     6541 ccctggtgcg tgggcaggat gtgcggaaat ccggtgattt cctgaatctt gtccttgagc
     6601 acgtaccagc tcttggcgcc ggcgtacgat tcgtcgccgc gcatgatccc ggcccactgc
     6661 aggctactca tggcgcccgt gccgctgtca gtcagcaggt cgatcaggac gtcctcggct
     6721 ttgatgagga acaggttaaa acccgcatcg accagcatgc gctcgcgctg ctcgcgcgtg
     6781 gtcatacgga tgggttcgac gactttgatg cgaaacggtt cgatgatcgt tttcatttct
     6841 caatccactt tggcgaaacg cccattttca actatggttg ccaagcctgc aatgcgtcga
     6901 aaacctgggc ataacttgtt ccaatcagca gcgtaacgcg atctcccggg ggatagaagt
     6961 cgatccggcc cggggtttga tccaagaata gcaacgagca agagctcaaa atcagcatcg
     7021 cccagatggc gagcacggcg gtacgggcgg cccgggcacg ccgcggggcg cctacacgcg
     7081 gggggagcag cgccgatgaa agcagcagca gcgcgagcag gccaaacgcg atgatcggcg
     7141 ttcgcggcag gccgggcgtg ctcggcgcga gtcgccggac atcgtccgcg aggctcgcat
     7201 cgcccagggt catccaggct agccagccca ggccgaatgc cgccacgatg cccagcacca
     7261 gcaattggtt cggtcgcttg ggtgccgtca gaaatgcccg gcgggcaaga accaacgcga
     7321 ctacattgac caacgccgcg gcgcacagcg ctcccagcat ccacatccgg agttgcagac
     7381 agctcgcaaa ccggccactt gaatccggcc gtagatcggg aacgtcgccg ctgccgagcg
     7441 ggtacggcaa aggcgggtgt tccgcggccg cagttcggta ggcagcacgc cactgtcgca
     7501 actgtgccgc gatttgggcg gccggcgcgg ggagcggcgc gaagctctgc tttccagggt
     7561 cctgcgcttc ctcgccggcg tcgctgatgg cctgcgccag caaatccgcg gccaggaggc
     7621 gcatgccggg cgggccgtcg tcgagcaccc actgctggag cagccttagc agcacgcggc
     7681 ggcacacaac atccgagccg tcttcggcgg ctgaccgcgt tcgagccaag gtgtccagcc
     7741 gcggcgcaag gtattggagc agcggtccgt gcggctggcc gatggtgtac tcggcctggg
     7801 ctcgcgccgc cccggggcgc aaacccagcg actcgtacat ctcggttcgg ctgtcttcga
     7861 gtggcgccag gtaatggcga aactgggccc gctccagccc gatcagcacg ggcgcgagtc
     7921 gcggccacca ctgctcgacc ggcgcagcgg ccctctccat ctccagcatc aggaaggcgg
     7981 ccagttgcac acgaccgatt tgcgtgtccg tcagttccgc gagcatgcgc tggagcgttt
     8041 cgcgtctggc ggggtaaacg tccgggtcga gcaaggcgct tgtgaagaga tggctcacgt
     8101 tggccgcgat cagcccctgc cgcgcggcgc gcgacatgcg ggcgtcggcc gcgcgctggg
     8161 cgcgttcgta atccagccac gtaagtcgcg ccaggcgcac gccggatagc ccgttccaga
     8221 actgccaggc gcgccagccg gcgatgaggc tcaacacgat cgccagggca agcaccacca
     8281 cacggcctct gtagacgcgc gtcttctgcg gagcggaacc ggccgatttc gcgggcgctg
     8341 ttcgtcccat gtgagcaagc taagccgccg cgcgcctggc ggcaacaaac gatgcgtttg
     8401 gctcgggtgt ggacggtacg cctccggggt tcgccgtctg ataatttcac gtcccaggcg
     8461 accctagtcg cgacgctatc cgggtgaaga cggcgaatat tctgccccgt attcagtact
     8521 cacttccgta gtcgcgccgc taattctagg accaaagaaa aacattgagc cccgacgccc
     8581 aagccgttaa gctaatcgaa caagcggatg ataagaacct gaggttgggc gccgcgcacc
     8641 ccgcgccgcg gccatgcggg cccccgcatg aatttacccg aggtaccgct catggagatg
     8701 ggaaatgtgc ccaagcgcgc gtttagccta aggttacgcg cgctagtcgc gatctgcgct
     8761 ttaatcgcgc tcggcgctcc gccgggcgcc ctggccgccg accgggttgt actctgcgaa
     8821 gagttcacca attacaactg actggagtgc gccagcgcgg gtcccgcgct tagccagttg
     8881 ctggatgtct tcccggactc cttgtccttc gtccagtatc actacggcga cgagtcggcg
     8941 ttgccctgga gtgatgcgcg ctggacgttc tatgacttcc actatacgcc ctccacggtc
     9001 ttcgacggcg ccgatgtgat gatcgggtcg ctgctggata tcgaactcca gtacaatcta
     9061 taccgcgtca accatttgca gcccgatcgg gccgccccga cagacgtcac gctcaccatc
     9121 cgcggcgagc cggtgagcgg ccagacctac caggccatcg tcgacgtcgg catcgagccg
     9181 gacggcgtcc ccaggacgct ccgcatctac atagtacagg tgttggacca ctggccgttc
     9241 accaaaccct actatcgcaa cggcttcaag caggccgccc cgaccgtgga cgtctcgctc
     9301 aatcctggtg agactcaaac gctacaggcg accttcacct ttgacgccga gagctgggcc
     9361 aaccgggaag acatcaaact gatcgcctgg gcccaactcc cgctggacac cgctcccgcg
     9421 gtggtctggc aatccgccac ccgccagtgg ccgctggtct cgtacccggg cgattgggat
     9481 ggcgacggcg tcgctgatac cgccgacaac tgcccgaggc gttacaaccc cgaccagact
     9541 gacagcgacg gcgatggcgt gggcgacatc tgcgacaagt gcctgaacca gcctgatccc
     9601 gcacaggacg atcctgacga ggacggaatc ggcagcgcct gcgataactg cccggttctg
     9661 cattctctca atcaatacga caccgatggc gacggcgtgg gcgatccgtg cgattcctgc
     9721 ccggaagtgc ccgcgccggc cggtgttgat cagtttgggc gtttgcgcgg cagcatggat
     9781 attgattgcg acgtggacgc tgacgatttg gctcttttcg gcccgtgcat cggcggccca
     9841 ggcgtgacga tcgccccgga cggctgcact cccgaagact tcgccagagc cgacctggat
     9901 ggcgacggcg acgtcgacct cagcgatcag gccatcttcg cccgcaactt ctccggctcc
     9961 ctgccgagcc cggatatgtt cgccggctat cagagctgca tcggctgcca cgccgagcag
    10021 gccgacctgt ggatacacac catccatcgg actgcatatg cgacggtcgc ggactcgccg
    10081 gacatcctga tctgcctgcc ctgccacacc gtcggttacg gcaagcgcag cggcttcgtg
    10141 gatatggaga ctacaccaca tctggctggc gtgcagtgcg aaagctgcca cgggccgggc
    10201 agcaatcaca acctcgaccc gatcgcctat ccgctgacgg tcagctacgg cacggcgctg
    10261 tgcggcgaat gccaccagtc ctgccacggc gtatgcggcg acaatagcca cccgcaactg
    10321 gagcaatggt cgcggtccaa gcacgcccgg gcgctacccg acatgaaagc ggatgagcag
    10381 gcctgggatg attgcctgcg ctgccactcg accgattacc gcctcaagcc cgacggcgag
    10441 aagcccggtc tgtacgaggc ccagtactcc atcgagtgta tcgcttgcca caatccgcac
    10501 ggcagcccga acaagggcca gcttcgtctg ccaccgagcc tgatttgcat tgattgccat
    10561 cacatggagg gtgagagcgg atactgggat ctaccggctc agccgcaggc tcagatactg
    10621 cacggccaac tcggcttcta tctggacgaa acgccgatgg aaggaccgta cagcatgcac
    10681 tggtggggca tcgcggacga gtgcgtggtg tgccacatgc acactgagcc gcgcacgccg
    10741 gaacacgagg ccgacagcgg ccacctcttc acctcgaacg tgcgggcctg catgccgtgt
    10801 cattccgagg aggttgcgcg gcagctagtg gacgagctgc actacgaggt gagcacccgc
    10861 tccagcctga tcgcccggca tttcgacccc ttcgacccgc tgtacgtcga tccgacttgg
    10921 ctgccgcccg acgaggtgat cgcctggatc ataagttact acaacaacca gttggtcatt
    10981 gcggaccgct cgttcgggtc gcataacccg gcttacatcc gcgccctgct ggccgaggcc
    11041 gaggcttacc tcggcatcga accctggccc gcgctaaggg ctgccggccg cgatgacctg
    11101 ctctggtcgt ttggcgtgca cgtgccccaa ccgaagaagg cggaggtgcc ttgatgacaa
    11161 ttactcgccg cgatttcctg aagctcgccg ccgccgccac cgcggccggg catctctcaa
    11221 ccgtcgcttt cgccaagctt cagaaggtcc tacgcgcccc aggcggcccg cgggtcgtct
    11281 ggctgcaagg ggccggctgc gacggctgcg cggtttcgtt cctcaatagc atccacttcg
    11341 ccggcgtcga cgatctgctc ctgaatacgc tggacgtgcg ctttcagaac aatctgatgg
    11401 ccgctaccgg tgagctggcc gtctccgccg cgaccggcgt tctccctgag cccggctaca
    11461 ttttgattgt cgaaggctcg atccccacgg ccgccggcgg gcgctattcc tacctctggc
    11521 cggaaatgac catgctggac ggcgttatca gcttcgctgc caacgccgca tacatcatcg
    11581 ccatcggcgc ctgtgcctgc tatggtggcg tcaccaaagg agctcccaac cccaccggcg
    11641 cccaaggcgt cggcgatgtc atcggcgccg attcacggct gataaacctg cccggttgcc
    11701 cgatgcaccc ggattggctg gtgtcgaccg tgacgcgtct gctcacgacc ggccgcgtgc
    11761 cgccgctcga tgcgcaccgc cgcccgctcg agtttttcgg gcgacttttg cacagcgact
    11821 gcccccgtcg ccacaagcac tgcggcgaaa tccaattggc ctcgcaactg ggcgagcccg
    11881 gctgcctgga atttctcggc tgcaagggca aacacaccaa cgccgagtgc cccatgcgaa
    11941 agtggaacag cagcggtccg ggcgagtccg gcgtcaactg gtgcatcggc gcaggcagtc
    12001 cgtgcatcgg ctgtgtcaat cccaactttc cggatggcat gtcgccgttc tatcaatatc
    12061 tgccgccgcc gcgcggcttg atcgaacggg gcgatgagga gcctgccgag actccggccc
    12121 cagcccacac cggccaagcc cctgcgagtg atgccaagaa aacccgctca gccggaggga
    12181 cccgctgatg ccaactacat ttactattga tccggtcacc cgcatcgaag gccacctgga
    12241 agtacagata acggtcgacc tggtcggcgg cgagcagaaa gtcgtcgacg cccgcagcgc
    12301 cggcacgatg tttcgcggct tcgaaatgat tctgcccggg cgcgacccgc gcgatgctgc
    12361 cctttatacc cagcgcatct gcggcgtctg ccccgtgccg catgctctgg ctgcctgtct
    12421 ggcgctcgac caggccttcg gcgtaacgcc cccggataac ggccggataa tgcgcaacct
    12481 cgtgctcggg gcgaacttca tcgattcgca cctgctgcac ttttaccatt tgtccgcgct
    12541 ggacttcatc gacaccactg gcgtgctcga catgccgcct tggacgccgc gctacgtcac
    12601 cgctgacatg ctcacggggc cggccgccgc cgcgttggtc gcccactatc gcaccgctct
    12661 ggcaattcgc cgcaaagcgc atcaaatggg cgcgatcttc ggcggaaaac tgccgtgcgt
    12721 cagctcgttt gtggtcggcg gctgcactga ccaggtgacc gccaagaaaa tccaggactt
    12781 ccgcgccctg ctcgccgaaa ttagggcctt catcgatgac gtctacctcc cggacgctgc
    12841 cgcgctggcc gccgccttcc caagctacta ctcgctcggt gccggccccg gcaatttgct
    12901 ggccttcggc gtcttcgatc tggacgccag cggcgccacc aaactgctcg gacccggccg
    12961 ttacaccaaa ggcgtgcccg gcacgttcaa cgctgcccag ataaccgaag acatcaccta
    13021 cggtcgctac aacggcgccg acgacttgaa ccccgccgtc ggcgtcacgc agccgaaccc
    13081 cgacaagcct ggcgcctaca cctggctcaa ggccccgcgc tattctaacc agccatacga
    13141 actgggaccg ctggcccgca tgtgggtcaa cggccggtat accaacggca tctcggccat
    13201 ggaccgcatc atggcccgcg cggccgaaac caagtttgtc gccgatgcga tggacggctg
    13261 gctcgatcag ctcgaacctg gacaaccggc gtatacgccg agcgagattg ccgccagcgc
    13321 tacctcggcc ggcctgacgg aggccccgcg cggcggacta ggccactgga tggacgtgac
    13381 gaaccacgtg atctcgcgtt accaggtcgt cacaccgacg agctggaacg ccgcgcccaa
    13441 agatcaccag aaccagcgcg gaccaatgga ggaggcgctg ctcggcgtgc cggtgccgga
    13501 aatcaccaag ccggtcgaag tgctgcgcat catccactcg ttcgacccgt gcctggcctg
    13561 cgcggttcat ctgctccggc cgggcgaaaa ggccggaacg gtcgtgcgtg tggcgacttg
    13621 gtcgtaagcc ggagtgaagt gttgactgcg acgccgcaac gtatgactga caacgcggag
    13681 cagggccggg cgccggtact ggtgctcggc atcggcaaca tcctgctgcg tgacgaaggc
    13741 atcgggccgc gcgtcgcgca gctattgcaa gccgcggctt tgccggccgg cgtagaggtc
    13801 tgcgacggcg gcacggccgg tttcaatctg atcgacgtgc tcgcgggccg ccgcaaggtc
    13861 atcgtgattg acgcgctcga tgccggtcac gcgccgggca gcattgtccg gctgactccg
    13921 caggacctga cgcggcagga tcggccgaac ctgtcgctgc acgatctggg cttgctggaa
    13981 gccttggaca tggcccaaca actgggtgag gcgccgcagg aagtggtcat cttcggcatt
    14041 caaccgcagg acttgagctg cggtctggac ctgtcgccca cgattcgcgc gtgtgtgccc
    14101 gagcttgtaa atctggtctt ggcggaattg aagggtgaaa acggccggca ggacggaggc
    14161 caggcgccgc cacaatctgt agtgttctcc gaagtgcagg aagaagtaaa atccggggaa
    14221 gaagcacagg aaagagcagg cgtagcatga agtacagtct cctcctgccg gccgcagtcc
    14281 ttctcgttct tacccagccc gcttgggccg agcctgcctg gggctccaat tgcctggcgt
    14341 gccatggcgt attgcagcca ggcctcatcg caatcatggg cgaagatgga accgccgacc
    14401 ccgacgaaag cgtcaccggc gcgccggatc gcggcctgtt gcccgtattc caggtctacc
    14461 ccggcaatag caagtctttg caggccgcac tggtcggcct gaacgaaggg gaccggtact
    14521 ccgtcgaact caagcgactg cgtttctcgg gcgtggagac cggctcgacg ctgctcttca
    14581 gcccggactg cgattggccc gagtggggtg attcgcctta ctacactcag ccggaagtgg
    14641 gatattgctg gggcgagggg ccgacgacct tcacgtacac catcggcact gacggcgaag
    14701 gcgcgtttga ctacttcgac ctggtgtttg cagtcgccgg caagtttgcg gacaccggcg
    14761 aattgttcta tgccgaggag catttctacc tgcaattgac ctggctgcgg ggcgacatca
    14821 actgcgacgg ctcagtgaac gttttcgaca ttgatccgtt cgtgcaggcc ctgaccgacc
    14881 ccgccggctt cctcggtgcg ctgccgggtt gcactatcga gaacgccgac attaatcgcg
    14941 acggttcagt agacgtcttc gatatcgacc cgttcgtgct gctgctgacc ggtggttgat
    15001 cctcgaataa cactcagact gccgtcgcca cgcggcccct acagttggaa tagtcctgcg
    15061 gctgaccgcg ttgaccagcg cggcgtccct tggcactgat gcttggcact gccatgcggc
    15121 caactcggct tgactccgcg cgggcgtcgc acgataacgt aagtggatgc ttacataaag
    15181 cccggaccgc ggcatgtgcg ggcccaagct gcctcctgcc ctgctgtcca ccaagtcgga
    15241 gaaccggctt atggaagaaa acgcggcagt cacggttcaa ccttcacccg tgggcgcggc
    15301 acccgatcgt ctgtcggcgt atctgcggct caaacgcgtt tttctgcgca ccctggtgat
    15361 ctcactgacg gtttgcgcac tggttgcggt cggcgcgctg ctgctgggaa cctttaacga
    15421 gaccacggcg aaagtgctga caacgctggg agcgctggcg gtacacagcg gcgccgcgat
    15481 gtcctgtgcc agtacgctcg aacgccggcg ctggccgaag ctgagcgcgg ttggactgat
    15541 acttttcgcc gccagtttct gcgtgctgat cacatgcatc tggtggccgg gttggttcga
    15601 tgaatcgacc atccgggcca ttctgacgtt tgggaccctg gccgggttct acattctggc
    15661 gattccatcg gcggacttgt gggagcggcg cacgcgccga gttctggcgg gggccggatt
    15721 gctggtttgc gcggctgcgt tcctcatggt actagcttgt atttgggcga cggactcgcg
    15781 caatgtagag tttgccaaag caaccggaat cgcgagcgtc atcgcgtttt cagtggcaca
    15841 tacggcactg ctggtgcggg tgcccgccgg tcgcggcgct gactggctgt tgatggtggc
    15901 attggggtgc gtctggctgc tggccgccat ggcctcggcc agcatcgcct gggagatcga
    15961 tgaggagttc tggtaccgct ggctgggcgc ggtcggcgtg ctggatgcgt gcagttcgtt
    16021 cgccctgctg atcatggcga agctgcggca ggtcggaaaa gtggaaaacc tgcaaagcgc
    16081 ggccgctcgc gtcgagctgc gttgcccgcg ctgcaccgcg cttcaaacgg tggacgccgg
    16141 cgtggccaag tgtagcgcgt gcgggctgaa gtttcggatc gaaatcgagg agccgcgctg
    16201 cgcgaagtgc gactacctgt tgtggcagtt gccggagcgc aggtgcccgg agtgcggaac
    16261 cgagttttga cctgcgagcg ggggtcgcgg ccgggttgta cgtgcgttcg tcggggcggt
    16321 cgtgcacccg cgtttggcgc tcgtcaccga cccccacggc agccgtagcc gccgggcagc
    16381 ctggccacgc tcgccttacc gacccccgtt ttcgggcctg taccgccctg gcgtgaccat
    16441 tatccgcctt gactccctgc cgcccggcaa ggtaccataa tcgtttctgc ggaaacgtct
    16501 tccgcgggtg aaaactgtaa tgtccggcgg ttggagcacc gccgctggcg cctgcaagcg
    16561 tcggcccgtg tcggccgcgc ccaggttctt ttgtttggga ggtgacgttt ggcatcggct
    16621 ctggtccgcg agctgttgga cgcgggcatt cacttcgggc accagtgtag ccgctggaac
    16681 ccaaagatga agccctacat ctacggcaag cggaatggca ttcacatcat tgatatacgc
    16741 gagacgctcc gcggcttgct gcgcgccagg aagtttctga cgcagttggt agccggcggc
    16801 gaagacgtgc tcttcgtcgg caccaagcgc caggcgcgtg aagtggtcga aaaggaagcc
    16861 gggcgctgca acatgcacta cgtcagcgag cgctggttag gcggcacgtt gacgaacttc
    16921 cgcaccatcc gtgggcggtt gcagcggtta gaggagctgg aatcgctggt cgcgagcccg
    16981 gtctgggaaa cggactattc gaaaaagatg aagtccaccc tctcgcggga actaaaaaag
    17041 attcagcgca acctgagcgg catccgcaaa atgaaccggc tgccgggtgc gcttgtgctg
    17101 gtggacgttc gcaaggaaca caacgcggtg cgtgaggcgc aggccctggg catccccacg
    17161 atcgccctgc tcgacacgga cagcgacccg gaaatgacga atattcccat tccgggcaac
    17221 gatgattcga tgcgctccat cgcgctggtg ctgcgctacc tggccgacgc ggtcgaagag
    17281 ggctttaagg ctcgacccga accgcgcccg caggccgaac aggccgaggg ctccgaattc
    17341 ggcgcgccgc ccccgcggcg ccgcggacgc cggccgatgc ccatggggcg cggcggtccc
    17401 ggtgggcctg aaccggccgg ttccgcggta gcgactaccg aagaggagtc gcccgccgcc
    17461 ccggaaggtg agccggccca cgactttggt gtgcaggctc aggggaacgc cccggcctga
    17521 acgtaacttg cgaatttttc gcggcgcccg ccctgggcgg ctccgcggac cacttagaaa
    17581 taacgattaa ggagcatcac gtggaaatta cccccgcgat ggtcatgcag ctacgcaacg
    17641 agaccggctt gcccatgatg gagtgcaagc aggccctgat cgcgtcgcaa ggcgataagg
    17701 agaaggccat tgaatggctg cggcgcaaag gcctggggca ggtcaccaag cgcgccgcgc
    17761 gcgagacgac cgaaggccgc atcgccgtgc acgtagaccc caagggcggc cgggcggcga
    17821 tcgtcgagct gctgtgcgag acggcgcccg tggcgaacac gaaggacttt atcgacctgg
    17881 cggcgcagat cgcgcggcac gccgtgcagc ttgacgcccc cacgccggag tcagtcctgg
    17941 atcagccgct gctggatgat gccgggcgca agttgcagga ctacatgcac gacgcgatca
    18001 accgcatccg cgagaacatc cgcattgcgc acgtcggcac gctgaccgga cacatcgggc
    18061 actacctgca ccacaacgcc caggtgggcg tgctggtgga gatgagcgcc gaagccccgg
    18121 acgcactgaa gtccgacgtc tgcatgcaga tcgccgccat gcgccccgtc tgtgcccggc
    18181 gcgaacaggt tccggccgaa ctggtcgaga ccgaacgccg cttggcgacc gaacaggcgc
    18241 agggcaagcc cgcgaacatc atcgagaaga tcgtcgaggg caagctcaac cgctggtacg
    18301 ccgagatggt gctggtcgag cagccgttcg tgaaggacga caagaagacc gtcggccaag
    18361 tgctgcgcga agcggtgccg aacctgacgg tgaaccgctt cctgcgcttc gaggtcgggc
    18421 aggaatagcg tagcgtgcgc ccgcgacgca cctgctaagg tgctcgccgc acctgcttca
    18481 tcaacatgaa caccagaagc ggccggtttt ttcaaaccgg ccgttttctt tttgactcct
    18541 gtggcggggg ccgtgtttcg agatccggcc tctggaccgg cgcgtcggcg ccggcaggcc
    18601 cagtcgtcgc tctattccgg cggcggctct ggtaactgcg gcgtgcgata ctgtttatcg
    18661 ttgacttggc agggtcccca tggtatacac gaggagtaac gtcagttcgt gcaatgcccg
    18721 gctggaatgc ggtcagcatt ctcttccggt gcaggcgtgc gttagaggca ccaacggctc
    18781 agttagtcga ggatcgggac gtaggttgct gagctaatga agactttctg cggacacgca
    18841 agtgcctgac gatacgggtt ataggcaaat catggtcaac ccgcccggca aatgcgcgtc
    18901 tggttgtact gcaaggcgcg agtatatgcc tgtctcagcg gtgaccaacg agcgctcttt
    18961 gagcgctcca ggaggagagt cggcatgctc acatgcagga tagcggcagt aagcctggcc
    19021 gcgctcgtca caacctgtgc ggcaatggcc gataaagagc catccgaggc gacgaatgcg
    19081 gccgccgaga ggagcgcggc tgtgagccaa gcccaggcat cgctggcccc ggcatccgta
    19141 acggggtgcg gaccagaatg cgacttttgc tggctcgaca ccgaattcga gaacaattgc
    19201 ccggaggact ggtacggcac gggaaacggc tgcgattgcg gctgccagtg ggttgacccg
    19261 gactgcgaca cgtctccgat aggcgcgtgc tgttcgccaa tccagggcac ccagtgcatc
    19321 gacgggctgg attacctgga ttgctgggac ttcggcgggg cgtacctcga gggcgtgacg
    19381 tgcgctgaga ccgattgcat tcgctgcccc ggctgcgacc ggtgctggac cgccacgatg
    19441 tttgagggcg gctgccctgc cgaatgggag ggcaatgggc cgtgtgactg cgggtgccag
    19501 ttcgaagaca cggaggattg ccagggttcg tggtccgctg gggcgtgctg ctcgccattc
    19561 cagggcgagt gcttcgacgg ccttacttcc gatgagtgcc ggaccttgga gatcggggta
    19621 tatgttgagg ggcagacctg cgccgagacc gactgcgtgc gctgtgcgcc ggagtgccac
    19681 ctctgctggg agggcaccat catggagggc gattgcgacc ccgcatggga aggcaacggc
    19741 ctgtgcgact gcgggtgcca gttcgaggat acgctggatt gcttcggccc gccgcccacc
    19801 ggggcgtgct gcctgccatt acaaggcggc cagtgcatcg acaacctgac ttttgaagag
    19861 tgctggaaca tgggctacgc ccctcttgag gggctgacct gcgccgagac cgattgtgtg
    19921 cgctgcgggc cggagtgcga ttggtgctgg atcggcacaa tcagcgaggg caattgcgac
    19981 ccttcatggg cgaacgacgg cgattgtgac tgcgggtgcc agtgggtcga cccggactgt
    20041 ccgggcggcg cttgctgccg cggtgacacc ggcgcatgcc gggtgacagt agaggccgac
    20101 tgcaccggcc ccggcgaaac ctactggggc gacggcactt cctgctcgcc caacccgtgt
    20161 ccgcagccgc tgccgaccgg cgcctgttgc gatagcagcg ctgcctgtac ggtctcaacc
    20221 gaagcggagt gcgcgggcgt ctaccagggc gacgggacca actgtacgac caccctgtgt
    20281 cccccgccca ccggcgcctg ttgctacgtc gcgggcgtct gcagcatcac gacggaagtt
    20341 ggttgcttag ggacctattg gggggacgga acggactgca gccccaaccc gtgccccggg
    20401 ccgaccggtg cctgctgcgc cattaccggt gaatgcacga tatcaacaaa ggcggagtgc
    20461 gatggcacgt attgggggaa ctggaccgat tgctcaccaa acccctgcgc gcggaccggc
    20521 gcatgctgcg acgggacggc ctgtacaatc tcaacacaag ccgactgccg cggggattat
    20581 ttgggagacg caacggtgtg cacgcccaac ccctgcgcct attggtaccg cgatgccgat
    20641 ggcgacggtc atggcgattt gaaccaggcc gtgcgggccg ctcagcgacc ggccggctac
    20701 gtggaagcct ccgacgattg cgacgacgcg aatcccgaca tttacccggg cgcagtggag
    20761 aactgcaacg agctggacga cgactgtgac ggccaagtgg atgaaggcgc ttgcgacagc
    20821 gaaagagccg ctcctggcgc ctgcgcgccc gccagcgccc tgctactgac tgcgacgctg
    20881 atcgggctgg cgcgcggccg ccgccggcgc tagatttact tcctcacaca ccggaaccca
    20941 tcatcgatat ccgggtcgtc cggctcgttc tttccgcgat tggccacgcg gcagccgaaa
    21001 gcgctgtcgt cccaactccc gccgcgcatc gatcgccacg tgcccgactc cggcccgcgc
    21061 gggttgtcat acgggctgat ttcgtaatag gcgctgtgat accagtcatt gacccattcc
    21121 cagacgttgc cggccatgtc gcacagtccg tagccgttga ccccattgct tgtctggtac
    21181 gtgtcggctg cgccgggcca gttccagtcc gttcgtaatt gcagcgctcc ggtgaagaac
    21241 ccgaccgggc tcgtataggg ttcgggcccc gcaccccata gtggagaatc gccagaggtc
    21301 ggattggtat catacagaaa agacgctgtg ctccagtagt tggcccgggc ctgctgaacc
    21361 gtgtcctgat cggaccacgg atagcggtgt cccgccgcgc cgccgcgcgc cgccctttcc
    21421 cactccgcct ccgtcggcaa acggtaaccg gccgcgtcga aatcgcattc ccacgtggac
    21481 aggttgtagc acacccccag gccctccatc gcactgcgcc agttgcagta cgccgccgct
    21541 ccataccagc ttaccgcggc catcgggtgg tgttccttgc ccgccactac gccaaactcg
    21601 ttgccatccc acgtaatgcg gctgatcgac cagcttgtat tcgtgtggca ataaggatgt
    21661 ccgccgccgg cctggtacac catgccgcca ctcactgaaa tcagcccgcc ctgcgcgagg
    21721 gcccagttca aagccgtggc atactgctgg ttcgtgacct cgcacacatc catatagaag
    21781 ccgtccacat agactgtgtg tacgggcagt tccgcgctgg tgccctcgcc gaaggcgtcg
    21841 cccatctgga attcgcccgc cggaatgaag accatttcgc ccgccgtggc cgcctgcgtc
    21901 gtaaacgacc aggtggggcc gggcgtttgg cccacgccgt tgccggcgat cacgctccag
    21961 tagtaggtcg tgctgtattg cagcttcgcc ggcgtccagt cgctgaagac ggtgttgccc
    22021 acaaacggcg gcggatcgct cgtcccgaag tacacgtcgt acgactcggc gtgggcggcg
    22081 tcgtcccagt cgagccatga atccagaggc tggtcgcctg cgccgtcggg cggaattggc
    22141 cccgagggct catccggcgc aaccagcgga ggcggcccgg tcgtaaacga ccagatgggg
    22201 ccggccgccg ttcccgcgct gttcttggag accacgttcc agtagtaggt cgtgctgtaa
    22261 ctcaatgttg cgagcgtcca cttgcttgca ctggtgttgc ccatgagtgg cggcggattg
    22321 tccgttccga aatacacatc ataagaagtc gcgcctgccg caacctccca gtaaagatcg
    22381 gactccaccg acacgtcgcc ggcgccatcg gcgggcgagg gcgcgccggg tgtgtccggc
    22441 agctttattg gcggctcagg cggactcggt tccgttttgt ccggcagcgt agtaaacgac
    22501 cagatcggcc ccggcgttga accggcgctg ttcgtggcga ccacgctcca gtaataggct
    22561 gtgccgtggc tcagcttcgc gaggggccaa tcgctggtaa cggtgttgcc ggccagcggc
    22621 ggcggattag ttgtgccgaa gtacacgtca taggacgtgg cccgcgcggc atcgctccag
    22681 tcgagtttca actctacagg aatatcaact gcgccgttgg cagggctagg ctgggttggc
    22741 gcatccggcg tcaagaggtc gtcccccacc gtgatttcac agctcagccc gaacacacct
    22801 gcgtcatccg cgtcgctgac acggacgcac tgaccgggaa aaacacggta tgagaccggt
    22861 acgacccagt cgtacgcccc gtcattctcg gttaagtcgg caatggtcgt cagcagcgag
    22921 cgatcggggc tgccggaagc gcttgcatcg taaagcaact cgacccgcac gttggcgata
    22981 gcgccggtgc tgctccatcg gatcgccaga gtgctgccgc cggggtaagt cggttttgcc
    23041 gctccgtcag ccggctgggt gaccgtgatc gaccgcgcct gctcaccgtc gccgggatcg
    23101 cgctcgccat tcctcggaca accggccagc atcgcgccca gcatcaggca gactaacccc
    23161 gtacgtggca gtttggccga ttgcattcgc gcctcctgtg acctcgagcc ggcccaagcc
    23221 ggcatgacgt ccggctcgcc tgaatctcgt cgaataacat tagcccccag gcgcgcgatg
    23281 tctcggacag gcaacgtgtc cgttcggatt tcgacccaag gacgcggccg tgccagcgca
    23341 tctcgttgtg ggacagtgac ttagctttcg gcgcggaagc caacgctgag cgcggagatc
    23401 ctgtacgccg aatccgagta acgctctcgc ctttgctgcg ctgaaatccc ggcacctcaa
    23461 atcggtgctg gggagatttc gcaatccgga aaatggcccc tgagctgcgc ttactgaaca
    23521 cgccccggcg caatcaccgt ccgaggggcg ccgatgccca caatcactac ccgcccgacg
    23581 tacgctgcgg cggagggcgc cgaaaatccg gccttggccg ccaccattgt cacggtcgcg
    23641 tcggctcgaa agcacggctg gctcgcctgc cccgtgtcac cgtccagccc cgacgggata
    23701 tcgacggcga tgcgtcgcgc acgcggcgcc gcgttggcaa tctcgaccag ttcggccatc
    23761 acgccgcgcg gcgccccgcg cgagccggtc ccaagcagtg catccacgat aatcgcggca
    23821 tgctgcgctt cggcgcgcac cttgtccaag ccggccgcct caaaggcgtg caggcgcggg
    23881 atgtccatgt gctcgacgat ccgcatattc gtggccgcgt cgcccttcag cttctcgggc
    23941 ggcaccgaca gcgccacggt taccggcacg cccgcattgc gcaagtggcg ggctaccacc
    24001 agcccgtccc cgccattatt gccagccccg cacagtatga gcacccgatc agcggccgga
    24061 ttaaccagac acccatacac aaactcggcg atgccgcgcc cggcgttctc catcagcaca
    24121 atgcccggca cgccgatctg ctcaatggcc aacgcgtcca actggcgaat ctgttcgcaa
    24181 ctaagcggct ctccaggcac gtacgggatg ctcatggcga tgctccgacg gattctcgtg
    24241 ctcacctcat cagccagcgc ttgaacgcgt gcgtcgtcgt atcgcgcccc agcttgccga
    24301 tcgcccacgt cagcggcacg tccttcgggc agaccttgac gcagttctcc gcattgccgc
    24361 agccggtgat gccgcccggt cccatcatcg cctcgttgcg gtagctcgcg gtcatcgccc
    24421 cggtcgggtg catgttgaac agcaccacct gcccaatgac ctgcgggccg atgaagtcgc
    24481 tgtgtgggtt gatctgcggg cacgcctcca ggcagcagcc gcaagtcatg cagcgcgaca
    24541 actcgtagcg tcgctcgttt acttccggcg gcacgatcgg cccggggccg agtggggcat
    24601 agctgtcaac cggaatccag gcccggactt tcttcagcgc gtcgaacatg cgcgaacggt
    24661 cggtgaacag atcgcggatc accgggaact tcgacatcgg tttcaggtcg atcaccggac
    24721 cggcccgcag aagttcatcg accagcgcgc tgcacgcctg ccgcacgaag ccgttaatta
    24781 ccatgctgca cgcgccgcat acctcctcga ggcagttact gtcccacaca accggcgtgg
    24841 tgtgctggcc gtcagcggtc accggatgcg ccgcgatttc ctgcaggacg gagatcacgt
    24901 tcatatccgg cttatatggc agctcgaacg tctgcgtgta aggcttggtc gcgggcccgt
    24961 cctggcgctg cacgcggact ttgaactttt ctagcatnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnngct ctgcccagac gcgctccact atttccgcgc ccttcatgcc
    25141 gtatgtccgc ggccgcggcg gtatcgagta caaatcaacc ggctcatacg tgatccgcgg
    25201 cccatccagc gtgtacgtcg ccagcgtggt cttgagccag cggtcgttct gctccttgaa
    25261 acgccggcac cattcctcgg cctctttgcg cagctcggcc ggccgctcgg cagtcggcgc
    25321 cggcagcgcg aattcgggct tgtaatgcgc cccgcggcac tcgtcgcgca gcaacgcgca
    25381 ctccgtaatc acccgcgcca gcaggatcat atcccccacg gcgcgcgtga aactgagcga
    25441 ctggttggtc cacaaaccgc cctggtccgc caccggggcg tggctatagc gctcggccaa
    25501 tttctccagt ttgtccagcg tgcggcgcag gtccgcgtta tgccggatga cggtgacgtt
    25561 gcgcgtcatc agttcgccca actcgcggtg tatgaggtac gcgttctcgc ggccctcgcg
    25621 cgccagcaac tgctcgtatt cctgtttgtt gcgggcgact tccccctcga acaggctcgt
    25681 ggaaactttg tccacgctgc cgcccggcag tgccgcaagg aacgaacgca tgcagcgccc
    25741 caccagcagc ccggcgaaga tgcagctcag cagggcattg gcgcccaggc gattggcccc
    25801 gtgatattgg taatcgcatt cgccgaccgc aaacaacccc ggcacgttcg tttgctgatt
    25861 ccgcggcgaa tcgacgcgca acccgcccgt gcgatcatca cgctcataat ccacccagag
    25921 gccgcccatg ctgtaatgga ccgccgggaa aatctgcatc ggctcggaaa gcgggtccac
    25981 gcccttgtac ttgcgataca tatccagaat cgcgccgagc ttgtgctcca gcacctcgcg
    26041 cttgacaccc gtggcgcgct ccgtcaaatc caggtatacc gcgtacgtgt gcgggctgac
    26101 gctatagccg tcccggcaaa tctggaatat ttcacgcgtg gcaatgtcgc gcggcagcag
    26161 attgccgtac gtcgggtact tctcttcgag aaaataccag cgctccgttt ccggaatttc
    26221 gttcggatgg cgccgatcgc cctgcttgcg cggcacccac acgcggcccg cctcgcctcg
    26281 ggccgcttcg gaaatcagcc gcagcttgtc cgcgcccgga atcgccgacg gatgtacctg
    26341 cacgaactcg ccgttggcgt agatcgcgcc ggccttgtac gcccgcgcgt tcgccgtacc
    26401 cgtgcagacc atcgacatcg ttgacttgcc gtaaaccagg cccggtccgc c
//
LOCUS       DAOSYS010000015        26141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1537_length_26141_cov_5.599210, whole genome shotgun sequence.
ACCESSION   DAOSYS010000015 DAOSYS010000000
VERSION     DAOSYS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 26141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26141
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_26141_cov_5.599210"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2644
                     /locus_tag="PLU99_01830"
     CDS             <1..2644
                     /locus_tag="PLU99_01830"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOO15824.1"
                     /translation="AVPLVTKSPAGDPVIELGPLLKSNFADIGWMASFDPDDPLGIFM
                     RGARINPGLSKWTKRKSFELNVEIGVELAIAARTPPGSYDKTLVHYSFWKLPRSDYQP
                     RIADDRVGYFVTAHRDWSKSTDERDIFNRYIHRWHLVKRDPSLEMCEPRQPITFYIEK
                     TVPVRYRRAVRDGILEWNKAFEKIGFVNAIEVRQQTDDNEWKDLDPEDMRYSFFRWIV
                     TGAGFAMGPSRANPFTGQLYDADIICDDSMVRYFQQEAETMLLPSALVARKFNDPAMR
                     QFLERFPHWRPPESLEMEEVVGEEPKWAELHERMRQRMSKLGKPYCEYCQGMKHQMQL
                     AGAVLVNFPPEVKERFLYEVLKEVVTHEVGHTLGLRHNFMASSIYSLEEIQKRRTTDA
                     PTCGSIMDYNPVLFTPENPTEGHFITPGIGPYDYWAIEYGYRPYDGSYKSARAEAEKG
                     KGDEKKEKAKDEAEKAGSAADETAQTPQPAPEKTENKISNLPDLSKLPPEIRDKLPEI
                     MAQLPPEVIQMLESGAIDFDEGADSEPPPMPKRGAREMSMPSAPAGEKGMLLDIARRC
                     TEPELAYATDEDCTMMSPDPRVNTFDMSSDPVGWARSRIELVDKRMANILEWAVKDQE
                     SWYHLRSAFVTLMFEKATTLDYVGRYVGGQYFTRAHRGDGAEGAPLVLVDAKLQREAL
                     RFLQENLYKDDFFQVAPDVLNHLVPPRWYHYGARVSYSMDFPIHDLVGFLQWWNLIDR
                     LYPNTLRRIYDAELKSTAPDRLTAAEYLQTIQDACWSDAVDLERLKKGAWSDAEPFLS
                     SYRRSLQREYLGLMESLVRYGPGGLLPPDVHAMLQYSLQKLSTNIGAVLNASGNKADF
                     ASQAHLSTCKSRIDRILTSELREY"
     gene            2806..3006
                     /locus_tag="PLU99_01835"
     CDS             2806..3006
                     /locus_tag="PLU99_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15825.1"
                     /translation="MCHDTEVYKEVIFADVFLRHCEVRRYTIEKLVFALQCVHPRLQM
                     RRGEAVHMEEHVYDGKLTGFTL"
     gene            complement(3043..3771)
                     /locus_tag="PLU99_01840"
     CDS             complement(3043..3771)
                     /locus_tag="PLU99_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO15826.1"
                     /translation="MSPERALLDFRHAPRWPALLGSLLCAGALAGCTECQFPPPIQKP
                     PAIVLTFDDGPLPGDLARPPDEYSADELLQPLRAILNTLERNNATAVFFIQGPGEETL
                     DAEGETAFATALREMYDGGQTLGYHAYHADPAIWIRPCLFPPLAALPMSADLDRLVGF
                     LNAVALPAGLEPDALFTPVFRQPFGGAGVSRVPAFLAAAARGWTYRGFRIDSADWAQN
                     TDSFPILAESFAALSEAQYVEYVV"
     gene            3924..5165
                     /locus_tag="PLU99_01845"
     CDS             3924..5165
                     /locus_tag="PLU99_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO15827.1"
                     /translation="MKAINPAFRLSMLAPVPYAWHLHSRTRALADQFARRGLRVRYVA
                     PPTPRAMLRHWIAPWRERRAGKVELVWPLPAPPLHWQQRLRLVNAIAALQARCIAPDS
                     DGTPHVIVAATPLWTPVVAHAPCDLLIYDCLDDPLVHARASSSEIYRRWHEELCRRAD
                     VIVAVSAALAERLRETCERRVVVCGNGVDADAFGALSNRESPAPSARLYETCDPALRD
                     WFAWWRAHPNQPVAGFVGSIDRWVDVELLAATARALPHVQFVVAGPERYRGLAAPLAG
                     IQNVHRIGLVSYKAVPALIATFDVGLIPFRAGPIAAAADPLKVYEYAAAGKAVVCSVP
                     LRLDDPDAPLVTAETPAAFASAVAAAAAAAAQSPNDSAAREQRIAFARRHTWAARAEQ
                     FLNLIAKERSRGVSPRNPAKP"
     gene            complement(5201..6331)
                     /locus_tag="PLU99_01850"
     CDS             complement(5201..6331)
                     /locus_tag="PLU99_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOO15828.1"
                     /translation="MIINDRILRGPRTGIGHYIAELTARVPLADPAIRLFGPFARLAR
                     SRERLDTLNHSRAPRQGARQDIAPWLRFVVETGYQAAFRGVGRALRCRIYHEPAHVPG
                     PWGGITLTTIHDLSVLRFPQWHPTDRVRWQERGLKRAVAQSAHFIAVSASTKAEMITL
                     LGVRPERVTVIHLGARETFHPRPRAAVEAWLAARNLPANYLLYVGAIEPRKNLPGLVE
                     AYAGLPTKLRAQFPLLIAGGQAWGAVDIAGIARSHGVGEQVRLLGYQGDESLAMLYGG
                     ARALVWPTFYEGFGLPPLEAMASGTPVIASAASSVPEVVGEAGLLVEPNDPAAIRAAM
                     QSVIEDDALAERLADWGPRRAAEFSWNRCAAEHAALYRRLAE"
     gene            6512..7846
                     /gene="glmM"
                     /locus_tag="PLU99_01855"
     CDS             6512..7846
                     /gene="glmM"
                     /locus_tag="PLU99_01855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOO15829.1"
                     /translation="MALMVGVSGVRGLVGQTLTPELVLEFARAYGTFLDGGRVVLARD
                     SRPSGPMYAAAAAAGLLAAGCQVTDLGVAMTPTVGRTIIEGKYAGGMSITASHNPAPW
                     NGLKFFTNLGFGPSTEDARQIAHIREQRRFRDAQSGFSAPQVDREAGQRHVRAVLAAR
                     EVDTSALRATKAVLDSVNGAGCEHSPALLKGFGIDLVHINGEPTGDFAHPPEPLSENL
                     SQLCQTVRTTRAAIGFAQDPDADRLAIVDENGTYIGEEYTLALAVESVLSRRPGPIAA
                     NLSTSRIIDDLAARYDVKVIRTPVGEAHVAAAMLQAGCVVGGEGNGGVIDPRICNVRD
                     SLSAMSLVLQLLATSGKTVSELVAALPRYASIKQKFACPRERIQAAVSAVTAKFADYN
                     PTTTDGVRVDFPAGWVHVRASNTEPIIRIIAEADSMAAADELVAQVRQAAGV"
     gene            7965..10520
                     /gene="glgP"
                     /locus_tag="PLU99_01860"
     CDS             7965..10520
                     /gene="glgP"
                     /locus_tag="PLU99_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOO15830.1"
                     /translation="MNHVRTFVVVPSLPEPLEPLRQLAYNLWWSWNHSARELFRRLDV
                     ELWERVYHNPVAMLWQIGQDRLEQVANDDAYMAQLQRVVDSFYVYLNSRTWYEERFPG
                     RQDEMVAYFSAEFGVHESLPIYSGGLGVLAGDHLKAASDLGIPLVGVGLIYRQGYFEQ
                     QLTEDGWQLESYPSYDFHQLAVTLVNDPFGNPLKIQVQLGRRQLTAQIWLAQVGRVKL
                     YLLDSDIPDNEPDLRMVTARLYGGDRRMRIQQEILLGIGGLQALRAVGIRPKVCHMNE
                     GHAAFMALERIRQAMEEFGLTYGEAREATVGGNVFTTHTPVPAGIDRFDPRLVIDQLG
                     WMASALGLSTDEFLALGREAGGLGDSDFCMPILALRLSFRSNGVSRLHGQVAAGMWQT
                     HWPKVPREEIPITYITNGIHTRTWISAEMAEIFDHYLGPNWAEAPPRTRLWERVEVIP
                     AAELWRVHVRRREHLIATVRTRLREQLKRRGAPPAEVKAADEVLDPSALTIGFARRFA
                     PYKRATLILRNLERLAALLRNNERPVQFIFAGKAHPADGAGKELIKQLCAICARPEFR
                     RRMVFLENYDMRLARILTQGCDVWLNNPLRLHEASGTSGMKGPPNGSLNLSCLDGWWP
                     EAYNGENGWAIGDGRVYDDLAYQDHVEAESLYNLLEREIVPLFYDRTVDDIPRKWLER
                     VKESIRTIVPVFSTIRMLREYTEVMYVPALQRAERLSADDFTVARRLNSWKQALSREW
                     HQVRIEEVDANAQERLKVGDKLPIHARVHLGTIRPENVAVEVYAGPVDSTGGISHGRP
                     APMQHTKENGNGVHEFDGVLPCTVSGQHGFAIRVVPAHEDLADRYEQGLIVWG"
     gene            complement(10536..11720)
                     /locus_tag="PLU99_01865"
     CDS             complement(10536..11720)
                     /locus_tag="PLU99_01865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOO15831.1"
                     /translation="MPSVIADKVAADMGSASFIREMFEKGRRLKAEFGEDNVFDFSLG
                     NPNATPPDAFFRALREAAEEHQPALHRYMPNVGFDEARAAVARFLRTEYGAPFEAADV
                     IMTSGAAGGMNVTLRALCNPGDEVLILTPFFPEYRFYVEQAGGRVVLVPTDADFQPDL
                     GKIEAAITERTRAIIINSPNNPTGAIYTEAKCQGLGALLARHDRPDRPIYILLDDPYR
                     RIVYDVDTCPTPLRHYPRTILISSYSKDISIAGERIGYIAVPHSVPQREKVLAAMTML
                     NRTLGFVNASAFMQRVIARCADARCDLDFYRQNRDLLCNALLSYGYDLKVPGGALYAF
                     PKTPIADDAEFIAVLVKHRVLAVPGRGFGRPGYMRLSFCVDRKTIERGLPALKAAIDE
                     VR"
     gene            complement(11934..12506)
                     /locus_tag="PLU99_01870"
     CDS             complement(11934..12506)
                     /locus_tag="PLU99_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15832.1"
                     /translation="MAIKALFSLPAAVLATVALLLVLQPGCGPVLLNQTAERSGSITV
                     RVINTTPYTAAFSYGSWDAWDRSPPGPVQMQQLVLAANSSSAPATVTCARNFSVGTRA
                     FIERAEVVDAPETLPNFNPDAFDSVVHFSNIPPGNPGAALPTVGTALGDARLLGWDYA
                     CGDELIFTLVEDPDAEGGFRIDFSVIPESE"
     gene            12775..14298
                     /locus_tag="PLU99_01875"
     CDS             12775..14298
                     /locus_tag="PLU99_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15833.1"
                     /translation="MRIAVLLTAVGAWMLSGASCRPLLDNTSDPAAKPTTLTLLIQSP
                     SSNATVAQGASVPITWTAYNATGQTATIDVYVESRTDLTQTTVADDISLTAGSLTQTS
                     YWDTTGLPGGEYVVYAAITAGADTQTQKAAGRITIDEFPAFEFTEPAEDATYTHGSDA
                     LKISWSASDPEGTGTVTVGLDSDSDPENGNELFIYEGTITADESESSFDWDGQDLLGD
                     DVAPGVYNLFALVGDAVNPERPVQAGRQITVVEGAPDETSVTLGILEPPYPATPENPE
                     PPKDHEFLAGTTMTIKYGVNKQVDALIDLKIDTDDVHTNGNELTIRAQWLVPADTESE
                     TFTWDGSLASGGDAPDGIYRLFIMANTGSATPEYGDDGEGLIYRRSYEKQPLIALEKP
                     AHMTTLQAGQTLLIKWRCVDDEGTATIRITIDDDPHPTGDPDDPDPADTLPEIDTLVT
                     DRACSSVEVEKTYMYQVPASLGPGVYYVFAYIRDPEAAGQEHYSVAPAPFVIRDPTR"
     gene            complement(14622..15356)
                     /locus_tag="PLU99_01880"
     CDS             complement(14622..15356)
                     /locus_tag="PLU99_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15834.1"
                     /translation="MSRQKFVSICCATLAAAVFVLPGRAAASGDAGEPPQSGLAARHS
                     LGSILYVPSQYPAIQAALDAASDGDEIRVADGTYTGPGNYDLDFHGKAVTLRSASGDP
                     ANCRINCAYEGRSFWFHSGETAASVVEGFRIYNGYPAAYTFDGRGGPVLAGFSSAPFG
                     HTPEHILEPSRTVLYSDPLFYAFVRESSPGSGFDPQAPRVAGKGNPGVRADRLAQEAQ
                     RRAFHERIDERGLRQGARDDVTALEA"
     assembly_gap    15588..15687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15895..18336)
                     /locus_tag="PLU99_01885"
     CDS             complement(15895..18336)
                     /locus_tag="PLU99_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO15835.1"
                     /translation="MWPFGRTSARRREIRRSKAERGWSWYRRLPAQVSLAPIGLAVGF
                     AILAAYLVNAGGPVFRIREGQTVSRALAARVEFRLEDTTRTIEMRGRARDNSPNYYEL
                     DVSLLKDIAGRLTNALQLAKVHADDPARLQEEARRTRIILDEAGRDELLRIAKVADAG
                     EQQREVARFQSAVERAVSSLKSQTPLLVEAAEAAARRTADRAKLIEPPEAGKDAVERE
                     LPVSKLITTAQPDAVDLVAAAASQAFAAPLQASMKNSIAGMLRREPDAAAPGAAGADS
                     PVRAIYRYAADLTSRAAQEAWAAVPKQHEVYAREQLLTDAGQISKKEHELLKAEHEEY
                     RKWLRRELPAEEELAALQRHQPDAYQRLIEEHQEVAAARASERLAVYGYGFLAFLIPL
                     GLTYYLRRPRRKPRDDPARQLTTTVVMLAVLAIARAVFVATGNPFYAVGVQAFAAGIL
                     GIVYARYCGPAMAAGLAVLITLATRQNMGFFLILMVVSGTFLVGLNNIRNRGGIVAVG
                     AAATLFALFTTLAAGLIDGQTLRFAFMAEALPAACTTMAAAFIIEGILPGIERVFKIS
                     TNMSLLEWGDASKPLLRLLAAEAPGTYNHSLLVGTLAENAAEAIGAQGLLARIGAYYH
                     DIGKINKPEYFAENQAVGDSRHERLSPAMSHLIITGHVKDGIEMAREYRLPPALHPFI
                     VEHHGTTLVEFFYHAANLQRKPGDPEVSEVNFRYSGPRPQSRETAVVMLCDAVEGAVR
                     AMSEPTPSRIEDTVSKIVQRRLMDGQLDECDLTFRELETIQRSVVKSLCAIYHSRVAY
                     PEREEAKSEAEVRVS"
     gene            complement(18361..19410)
                     /locus_tag="PLU99_01890"
     CDS             complement(18361..19410)
                     /locus_tag="PLU99_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO15836.1"
                     /translation="MELSLTLKEPHKRAALFGPGDAYLRRIRQTLDVQLQARNNTVRI
                     VGEPTVVARAAAVVERLQELLRGREYIDERALEQTLADVVGAQERPSGEALAVFTPHV
                     VIAPRTEGQRRYVRAMLDYDMVFCSGPAGTGKTYLAVAVAVSLLKSGHTNRIVLVRPA
                     VEAGEKLGFLPGDLQQKVNPYLRPLFDAMHDMMTFDQLKRFMVNDIIEVIPLAYMRGR
                     TLNNAVIILDEAQNCTPSQMLMFLTRLGHHSKMIVTGDDSQIDLEPGQASGLVDAVSR
                     LRGVEGIAILRLTEVDIIRHKLVQAVVTRYADEPLTAGRPTRSDNRRRTLPPTEPAPD
                     SGLPSSSPAGEQTKQ"
     gene            complement(19640..20242)
                     /locus_tag="PLU99_01895"
     CDS             complement(19640..20242)
                     /locus_tag="PLU99_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO15837.1"
                     /translation="MSLRYVFVTASLLCAASLAWADIVTVPEFTGYSTEGFEDVLSPG
                     GHPSPVEIFRGRCSMDDSLSHTVVIANYWQGPGGTVMPRFGNWMGGTTAGDTVLQFTV
                     PVTDFGTYITTNAAAEDGTITFSDASGAVIGSLPLTVVPGTWGWQGWHSDTPISTIVM
                     TTNAGMGWGLIFDAMQANWVPEPASLGLLAMAALMLVRRR"
     gene            20636..21607
                     /locus_tag="PLU99_01900"
     CDS             20636..21607
                     /locus_tag="PLU99_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15838.1"
                     /translation="MLASQHAFFGNILDYAGMFPPAQLPLDASIRNYLQYQSEPESWL
                     LARFICPAGRLHDLRPYVTKHVSGRSPLRISVLGSGGDTAATFLDNLNSDLEATADFR
                     QEFGPAVAVECFEARLPAGDLLEPADLLTMISAAFAAQGLTALPRFFELPATANWPRI
                     ADTLATLHHAASPEGQGVAGGRALNGLKLRCGGSEPAAIPSAAQVATAIGACLSANVP
                     LKFTAGLHQPVRCVAAALGTRVHGFLNLFAAGILGASAELEYDDLVTIIEEEDVTQFS
                     FSDDLFAWNGAKATVSEIAHARHRNVISFGSCSFDEPRDGLRELGYL"
     gene            21610..22905
                     /gene="fahA"
                     /locus_tag="PLU99_01905"
     CDS             21610..22905
                     /gene="fahA"
                     /locus_tag="PLU99_01905"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010497965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOO15839.1"
                     /translation="MAENIMRSPRSEQRSFIETDPESHFPIQNLPYGVFKRPGEEPRV
                     GVAIGDMVLDLAALEERGFFEAAALGATPVFRRPSLNYFMSLGRPAWQRVRAIISRLL
                     SADEPRLRDDPELRRAVLMPQASVEMCLPAEIGDYTDFYSSLEHAMNVGTMFRGPENA
                     LPPNYRWMPIAYHGRASSIVLSGADLHRPRGQTRREAGAAPEFGSSRRVDFELEMGFF
                     VGPGNELGCPIPADQAAEHIFGLVLVNDWSARDIQQWEYVPLGPFLAKNFGTSISPWV
                     VTLDALEPFRCAGPEQDPPPLPYLRTEGKQAFDIHLEVTLQPAASPLPHVICRSNYKY
                     LYWNMCQQLAHHTITGCNLRPGDLLASGTISGPAPGSYGSLLELAWRGERPLQLPNGQ
                     RRSFIEDGDRIVMTAWCQGDGYRVGFGEVTGRILPPLDP"
     gene            complement(22924..24126)
                     /locus_tag="PLU99_01910"
     CDS             complement(22924..24126)
                     /locus_tag="PLU99_01910"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOO15840.1"
                     /translation="MLHRHTLGRVPAKHHTALYDEDGRLLMEQMITRDGFNGPYSILY
                     YRTPPTDEFEVAALRLPGFCPVELVAEQPLHRRHIRTAELAPGGDFLTGRRTLMVTRH
                     LHLGVVKPTESPQRYFSNGDGDELFFVTQGSGYVDSVYGVLPFREHDYILIPKSTPYR
                     LCLAGDRGTLLFFEGRPRLGIPSDYRNRWGQLTDYAPYSHRDFRLPQELLAVEPEQRS
                     GPFELVVKASDELTVHRYKHFPLDVVGWDGVLYPVAFSIHDYQPKTGLVHLPPTIHTT
                     FSGEGFVVCSFVPRKVDYHEKAIPCPYAHSSVDMDEVLYYVAGDFTSRKGIRPESISL
                     HPQGVTHGPHPGTYEKSIGTERTNELAVMCDTHEPLRLTTVAAGLEDKDYHTSWVTRA
                     GDSTWTSK"
     gene            complement(24191..25105)
                     /locus_tag="PLU99_01915"
     CDS             complement(24191..25105)
                     /locus_tag="PLU99_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO15841.1"
                     /translation="MTKLLTALLLGLLLFAAGCIPLIPRKQFLSLANDVAFAPELTCE
                     QLKVDFGVPDLPTVKDPAELGIPFERAFVKTADDQILQVWYVPAQPERGLVLLAYGAV
                     GELPCYLLLTKHLYADGWSMVMYDFQGFGGSTGRPSFNTLVTDHTAVLDWTLARTGRD
                     KVTLMGISVGTLPTVAQAAARPEAVNAVVLDGLLVLRLQVRRAWLLIGGRVEHYMKVF
                     DPRLRIEEQLVRVHQPICAVVYGRDEYAKPGQIRKILSRAPGEVTIIEFPNLMHARGP
                     YLATDEYFARLQAFLAGVWEGSGDVNSR"
     gene            25439..>26141
                     /locus_tag="PLU99_01920"
     CDS             25439..>26141
                     /locus_tag="PLU99_01920"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOO15842.1"
                     /translation="MSREKVHASRYGLDTIGLAHQATAFWNLPPATLIVEALRRREAT
                     LADNGALVALTGMRTGRSPRDKFVVKEPETESQVNWGKVNIPIPPEGFASLRDKVYHH
                     LCYEDVFVQDLYAGAHPAYRLNVRVITELAWHSLFARQLFIRPPRDATADFKPDFTVV
                     CAPNCLADPQRDRINSETFVVISFSRKLVLIGGTRYAGEMKKSIFTVLNYVLPAEGVL
                     SMHCAANSGHAGDTAL"
BASE COUNT         4859 a         8296 c         8216 g         4670 t
ORIGIN      
        1 agcggtgccg ctggtaacca agtcccccgc cggtgacccg gtgatcgagc tggggccgct
       61 gttgaaaagc aacttcgccg atatcggctg gatggcgtcc tttgacccgg atgacccatt
      121 gggcattttc atgcgggggg cgcggatcaa ccccggcctg agcaaatgga ccaagcgcaa
      181 gagctttgag ctgaacgtcg agatcggcgt cgagttggcg atcgcggccc gtaccccgcc
      241 gggctcgtac gacaagacgc tggtgcacta cagcttttgg aagctgccca ggagcgatta
      301 ccagccgcgc attgcagacg accgcgtcgg ctacttcgtg acggcccacc gggactggtc
      361 caagtcgact gatgagcggg acatcttcaa ccgctatatc caccgctggc acctggtgaa
      421 gcgcgacccc agcctggaaa tgtgcgaacc gcggcagcca atcacgttct acatcgagaa
      481 gacggtcccg gtgcgttacc ggcgggcggt gcgtgacggc atcctcgagt ggaacaaggc
      541 attcgagaag atcgggttcg tcaacgcaat tgaagtgcgg cagcaaacgg acgacaacga
      601 gtggaaagac ctcgatccgg aagatatgcg atacagcttc ttccggtgga tcgtgacggg
      661 cgccgggttc gccatgggac cgagccgggc aaacccattc accggtcagc tctacgatgc
      721 ggacatcatc tgcgacgatt caatggtccg ctacttccag caggaagcgg agacgatgct
      781 gctgccgtcg gcgctggtgg cccgcaaatt caacgatccc gccatgcgcc agttcctgga
      841 gcgctttccg cactggcgcc cgccggagtc gctggagatg gaagaggtgg tgggcgaaga
      901 gcccaagtgg gcggagttgc acgagcgtat gcggcagcgc atgagcaagc tgggcaagcc
      961 gtactgtgag tactgccaag gcatgaagca ccagatgcaa ttggctggtg cggtgctggt
     1021 gaacttcccg ccggaagtga aggaacgctt cctgtatgaa gtgctcaaag aagtggtgac
     1081 gcatgaagtg ggccacacgc tcggactgcg gcacaacttc atggcgagtt cgatttactc
     1141 gctcgaggaa atccagaagc ggcgcaccac cgacgcgccg acctgcggct cgatcatgga
     1201 ctacaacccg gtactgttca cgccggaaaa cccgaccgaa gggcacttta tcactcccgg
     1261 catcgggccg tacgactatt gggcgatcga gtacggctac cggccctatg acggcagcta
     1321 caagtcggcc cgagccgagg cggaaaaggg caagggggac gaaaaaaagg agaaggctaa
     1381 ggacgaagcc gagaaggccg gcagcgcggc ggatgaaacg gcccaaacgc cgcagccggc
     1441 ccctgagaag acggaaaaca agatttccaa tctgccggac ttaagtaagc ttccaccgga
     1501 gattcgggac aaactgcccg aaatcatggc gcaactgccg ccggaggtga tacagatgct
     1561 ggaatcgggg gcgatcgact tcgacgaagg ggcggatagc gagccgccgc cgatgcccaa
     1621 gcgtggggcg cgtgaaatgt cgatgccgag cgcgcctgcg ggggaaaaag gcatgctgct
     1681 ggacatcgcg cggcgctgca ccgagccgga gctcgcctac gcgacggacg aggactgcac
     1741 catgatgtcg cccgacccgc gcgtgaacac gttcgacatg tcgagtgacc cggtgggctg
     1801 ggctcgcagc cggatcgagt tggtcgacaa gcgcatggcg aacattctcg aatgggctgt
     1861 caaggatcag gagagctggt accacttgcg ttcggcgttt gtcacgctga tgttcgagaa
     1921 ggccacgacg ctggactacg tcgggcgcta tgttggcggg cagtacttca cgcgggccca
     1981 ccggggtgat ggagccgaag gcgccccgct cgtgttggtt gacgcgaagc tccagcgcga
     2041 ggcgctgcgg ttcctccaag agaacctgta caaggatgac ttcttccagg ttgcgccgga
     2101 cgtgctgaac cacctggtgc cgccgcgctg gtatcactac ggcgctcggg tcagctacag
     2161 catggatttc cccatccacg atctggtcgg gttccttcag tggtggaacc taattgaccg
     2221 gctctacccc aacacgcttc gccgcatata cgatgccgag ctcaagagca ctgcacccga
     2281 tcggctgacg gcggcggaat atctgcagac cattcaagat gcctgctggt cggacgcggt
     2341 tgacctggaa cggctgaaga agggggcgtg gtcagacgcc gagccgttcc tgtcgagcta
     2401 tcggcgctca ctgcagcgcg agtacctggg gttaatggaa tcgctggtga ggtatggtcc
     2461 cggcgggcta ttgccgccgg acgtgcacgc catgttgcag tactcgctgc aaaagctgag
     2521 cacgaacatc ggcgccgtgc tgaacgcgtc ggggaacaag gcggacttcg cctcgcaggc
     2581 gcatttgtcg acctgcaagt cgcgcattga tcgaattttg acgtcggagt tgcgggagta
     2641 ctaacccaag ttagagagcg gcaggcaagt taaggtagtc actgccttcg aacggaaacg
     2701 gccctggcgc tagactggtg ccagggccgt ttcgccccag aatcaagagc cggcttactg
     2761 tgagggcgtt gcgagaagtc tgctttcgcc cggcggcgcg ggcaattgtg tcacgacact
     2821 gaggtctaca aagaagtcat cttcgcggac gtattcctca ggcactgtga agtccgccgt
     2881 tacaccatag agaaactggt attcgccctg caatgtgtcc acccacgtct gcagatgcgc
     2941 cgcggtgaag ctgttcacat ggaagagcac gtctatgacg gcaaactcac cgggttcacg
     3001 ctgtaactcg tccgcagtgt gccgcagccc gtcctgcagc cgctagacaa cgtactcgac
     3061 atactgcgct tcactgagcg cggcgaacga ctcagccaga atcggaaaac tatcggtgtt
     3121 ttgcgcccaa tccgcactat cgatgcgaaa tccgcgatag gtccagccgc gcgccgcggc
     3181 cgccaggaag gccggcacgc ggctgacgcc tgcgccgccg aacggctggc ggaaaacggg
     3241 cgtgaagagg gcatccggct caaggccggc cggaagcgcg acggcattga ggaacccgac
     3301 caaccggtcg aggtcggcgg acatcggcag ggccgccagc ggcggaaaga ggcagggccg
     3361 gatccagatg gcggggtcgg cgtggtaggc gtggtagccg agcgtctgcc cgccgtcgta
     3421 catttcgcgc agggccgtcg cgaacgcggt ttcgccttcc gcatcaagcg tttcttcgcc
     3481 ggggccctga ataaagaaga ccgccgtggc gttgttgcgc tccagcgtgt tgagaatcgc
     3541 gcgtaggggc tggagcagct cgtccgcgga atattcgtcc ggcggccgcg ccaagtcgcc
     3601 cggcagcggt ccatcgtcga acgtgagcac gatcgcgggc ggtttctgga tgggcggcgg
     3661 aaactggcat tcagtgcagc ccgccaaggc gccagcacac agcaggctgc cgagcagcgc
     3721 aggccagcgc ggcgcgtgcc ggaaatccag caatgctctc tcaggcgaca tcgaatctgc
     3781 atcatactag aaaataagaa cgggtcggga gtctcagtat ttagcgctcg cgaaccacca
     3841 atgagccgcc tccacttgac agccgcaggc gatctgaaag actgacgccc tgccgccggt
     3901 tggtttccag gtgcataggc gcgatgaaag ccataaaccc cgcattccgc ctttcgatgc
     3961 ttgcgcccgt gccctatgcc tggcaccttc acagccgcac gcgcgcttta gccgaccagt
     4021 tcgcccgccg cgggttgcgc gtacgctacg tggccccgcc cacgccgcgc gccatgttgc
     4081 ggcattggat tgcgccgtgg cgcgagcgcc gggccggaaa ggtggagcta gtctggccgc
     4141 tgcccgcccc gccgctgcat tggcagcaac gcctgcgtct ggtcaacgca atcgcagcgc
     4201 tccaggcgag gtgcatcgcc cctgattcgg acggcacgcc tcatgtcatc gtagccgcga
     4261 cccctttatg gacgccggtg gtcgcgcatg cgccgtgcga cctgctgatc tatgattgcc
     4321 tcgatgatcc gctggttcac gctcgcgcta gcagtagcga aatttatcgt cgctggcacg
     4381 aggagttgtg ccgccgggcc gacgtgatcg tcgcggtttc tgccgcgttg gccgagcgcc
     4441 tgcgcgaaac gtgcgagcgc cgggtcgtgg tttgcggaaa tggcgtagac gcggacgctt
     4501 tcggcgcgct ttcaaatcgc gaatcgccag ccccttcagc ccggctctat gaaacatgcg
     4561 atccagcatt gcgcgactgg ttcgcctggt ggcgcgcgca tcccaaccag ccagtcgccg
     4621 gcttcgttgg ctcgatcgac cgctgggtag atgtggaact tctggcggcc acggcccgcg
     4681 ctctgccgca cgttcaattc gtcgtggccg gccccgagcg gtaccggggc ctggccgcgc
     4741 cgctggccgg aattcagaac gtgcatcgca tcgggctggt ttcttataag gctgttccgg
     4801 cgctgatcgc gacgttcgat gtggggctga ttccgttccg ggccgggccg attgccgcgg
     4861 cggccgatcc gctgaaggtc tatgagtatg cggccgccgg gaaggcggtc gtgtgctccg
     4921 tgccgttgcg gctcgacgat ccggatgcgc ctctggtcac cgcggaaacg ccggcagctt
     4981 tcgcaagcgc cgtcgccgcc gcagccgccg ccgcggcgca atcaccgaac gattccgccg
     5041 cacgcgagca gcgcatcgcg ttcgcacgtc gccatacatg ggcggcgcgt gcggagcaat
     5101 tcctcaacct gattgcgaaa gagcggagcc ggggcgtgtc cccccgaaac cccgccaagc
     5161 cgtagcagag ccggggcgtg tcgcccggga ttaccaccaa tcattccgcc aggcggcggt
     5221 aaagcgccgc atgctccgcg gcgcagcgat tccaggagaa ctcggcagca cgccgcggcc
     5281 cccagtcggc caaacgctcc gccagggcat cgtcctcgat caccgattgc atcgccgcgc
     5341 ggatcgccgc cggatcgttc ggctcgacca acagccccgc ctcccccact acttcgggca
     5401 ccgaactcgc cgcactcgcg atgacgggcg taccgctggc catcgcctcc agtggcggca
     5461 acccgaagcc ctcatagaac gtcggccaga ccagcgcacg cgcgccacca tagagcatgg
     5521 ccagcgactc gtccccctga tagccgagca gccgcacctg ctcgccgacg ccgtgggagc
     5581 gagcgattcc cgcaatgtcg accgcacccc aggcctgccc gcccgcgatc aagagcggaa
     5641 actgcgcgcg cagctttgtc gggagtccgg cgtatgcctc gaccaatccg ggcaggttct
     5701 tacgtggctc aattgcacca acgtacaaca aatagttcgc cggcagattg cgagctgcca
     5761 gccaggcttc taccgccgcg cgcggacgcg gatggaaagt ctcccgcgcg ccaagatgaa
     5821 ttacggtgac gcgctccggc cgtacgccca gcagtgtgat catctccgcc ttcgtggagg
     5881 cggaaacagc tatgaaatgg gcgctttgtg ccacggcgcg cttcaggccg cgttcctgcc
     5941 accgcacgcg atctgtagga tgccattgcg ggaatcgcag caccgacaag tcgtgaattg
     6001 tcgtcagcgt gattccgccc caaggtcctg gcacgtgcgc cggctcgtga taaattcggc
     6061 agcgaagagc gcgcccgacg ccgcggaaag cggcttgata gccggtttca acgacaaacc
     6121 ggagccaagg cgcgatgtcc tgccgtgcac cctgccgcgg tgcgcggctg tggtttagag
     6181 tatctaaccg ttcgcgactt cgtgcgagcc gcgcgaacgg cccgaatagc ctgatcgccg
     6241 ggtcggccag cggcacgcgc gccgtgagtt ccgcgatata gtgaccaatc ccggtgcgcg
     6301 ggccgcgcag gatgcgatcg ttgatgatca cccttagaac ttggccggac atggctgccg
     6361 acggtagccg agccaaaaac gccccgcaag ccgcggttgg cggcagatgg ctggcgtggc
     6421 atactgcccc atctactttg cgctgcgagt cagaggcggc cgggcgcatc tggcttacgg
     6481 ctgagagctg ctcagagctt ggaggacgaa tatggcgttg atggttggag tctctggcgt
     6541 tcgcgggttg gttggtcaga cgctgacgcc ggaactggta ctcgaattcg cccgcgccta
     6601 cggcacgttt ctggatggcg gccgggtcgt gctggcccgc gattcccggc ccagcggccc
     6661 gatgtacgcc gccgcggccg cggccggatt gctggccgcc ggctgccaag tgacggacct
     6721 gggcgtggct atgactccga ccgtgggacg aaccatcatc gaaggcaaat acgcaggcgg
     6781 aatgagcatc accgccagcc ataaccccgc gccctggaac ggcctgaaat tctttaccaa
     6841 cttgggcttc gggcccagta cggaagacgc gcgccagata gcgcacattc gcgagcagcg
     6901 ccgcttccgc gacgcgcaaa gcggcttcag cgctccgcag gtcgaccgcg aggccggcca
     6961 gcggcacgtg cgcgcggtgc tggccgcgcg cgaagtggac acctccgccc tgcgcgccac
     7021 aaaggccgtc ctggatagcg tcaacggggc cggctgcgag catagcccgg cgctgctcaa
     7081 gggtttcggc atcgatctgg tgcatatcaa cggcgaaccg acgggcgatt tcgcccatcc
     7141 gcccgagccg ctcagcgaaa acttgagcca actttgccaa accgtgcgca ccacgcgggc
     7201 cgccatcggc ttcgcgcaag acccggatgc ggaccgcctg gccattgttg acgaaaacgg
     7261 aacttacatc ggcgaggagt acacgctggc cctggccgtc gagtcggtgc tctcgcgccg
     7321 cccggggcca atcgccgcca acctcagtac gtcgcggatc atagacgacc tggcggctcg
     7381 atatgacgtg aaggtcatcc gcacccccgt gggcgaggcg cacgtggccg cggcgatgtt
     7441 gcaggccggc tgcgtggtgg gcggggaggg caacggcggt gtcatcgatc cgcgcatctg
     7501 caatgtgcgg gacagcctgt ctgcgatgag tttggtgctt caactgctgg ccacctccgg
     7561 taagaccgtc agcgagctgg tcgccgccct gccgcgctac gcctccataa agcagaaatt
     7621 cgcctgcccg cgcgagcgga ttcaggccgc ggtgagcgcg gttacagcca aatttgcgga
     7681 ctacaatccg actacgaccg acggggtccg ggtggacttt cccgccggct gggtgcatgt
     7741 gcgggcctcg aataccgagc cgataatccg gatcatcgct gaggcagaca gcatggcggc
     7801 ggccgacgaa ctggtcgcgc aggttcggca ggcggccggt gtgtaagcta cggaaccgtg
     7861 ctttatgatg cggttgttca ctcgcggcct gggtggggcc gaaagtaaga acggtcgtag
     7921 gaccgatata gtttgccgtg cactcaattg atgggtagga ctcgatgaat cacgttcgca
     7981 ccttcgtcgt cgtgccatca ttgcctgagc cgctcgagcc gctacggcag ctcgcctaca
     8041 acctctggtg gtcttggaac cactcggcgc gcgagctttt tcgccggctg gacgtcgagc
     8101 tgtgggagcg cgtctaccac aacccggtcg ccatgttgtg gcagatcggc caggaccgcc
     8161 tggagcaggt ggccaacgac gatgcctaca tggcgcaact gcaacgcgtc gtcgactcgt
     8221 tttacgttta tctgaacagt cgcacctggt acgaggaacg ttttccgggc cgccaggacg
     8281 aaatggtcgc gtatttttcg gccgaattcg gcgttcatga gtcgctgcca atctattcgg
     8341 gcggcctggg tgtactggcc ggcgaccatc tcaaagcggc ctcggatctc ggcattccgc
     8401 tggttggtgt cggcctgatt taccgccagg gctatttcga acagcaactg accgaggacg
     8461 gctggcaact cgaaagctat cccagctacg acttccatca gcttgcggtc acgctggtca
     8521 acgatccctt cggcaatccg ttgaagattc aggtacagct cgggcgtcgg cagcttacgg
     8581 cccagatctg gctggcccag gtcggccgcg tcaaacttta cctgctcgac agcgacatac
     8641 ctgataacga gcctgacctg cgcatggtca cggcccggct gtacggcggc gaccgccgca
     8701 tgcgcattca gcaggaaatc ctgctcggca tcggcggttt gcaggcgctg cgcgccgtcg
     8761 gaatccgccc caaggtttgt cacatgaacg agggacatgc cgcgttcatg gccctggagc
     8821 gcattcgtca ggccatggag gaatttgggc tgacttacgg cgaggcgcgc gaagcaacgg
     8881 tgggcggcaa cgtctttacg acgcatacgc ccgtgccggc cggcatcgac cggttcgatc
     8941 cacgcttggt gatcgaccaa ctcggctgga tggcgtcggc cctcggccta tccaccgacg
     9001 agtttcttgc cctgggccgc gaggccggcg gtctgggcga cagcgatttc tgcatgccga
     9061 ttctcgccct gcgcctgtcc ttccgcagca acggcgtcag ccgcctgcac ggccaggtgg
     9121 ccgccggcat gtggcagacg cactggccga aggtgccgcg cgaggaaatc cccatcacct
     9181 acatcaccaa cggcatccac acgcgcacct ggatttcggc ggaaatggct gagatcttcg
     9241 atcattacct cgggccgaac tgggccgagg cgccgccccg gacgcggcta tgggaacgcg
     9301 tcgaggtcat tccagccgcc gagttgtggc gcgtccacgt gcgccgccgc gagcatctga
     9361 tcgccacggt tcgcacccgc ctgcgcgagc agctaaagcg gcgcggcgcg ccgccggccg
     9421 aagtgaaggc ggccgacgaa gtgctggatc cttcggccct gacgatcggc ttcgcgcggc
     9481 gcttcgcgcc gtacaagcgc gctaccctga tcttgcgaaa cctcgaacgc ctggccgcgt
     9541 tgctgcgcaa caacgagcga ccggttcagt tcatctttgc cggcaaagcc cacccggccg
     9601 acggcgcggg caaggagctc attaagcagt tgtgcgcgat ctgcgcccgc cccgagttcc
     9661 gccggcggat ggtgttcctc gaaaattatg acatgcgcct ggcgcgcatc ctcacgcagg
     9721 gctgcgacgt ctggctgaac aacccgctgc gcctgcacga ggctagcggc accagcggca
     9781 tgaagggccc gcccaacggc agtctgaacc tctcctgcct cgatggctgg tggcccgagg
     9841 cttataacgg cgagaacggc tgggcgatcg gcgacgggcg cgtgtacgac gacctggcct
     9901 accaggacca tgtggaggcc gaatcgctct acaacctgct agagcgcgaa atcgtgccgc
     9961 tcttctatga tcgcacggtg gacgacatcc cccgcaagtg gctggagcgc gtcaaagagt
    10021 caatccgcac catcgtcccc gtcttcagca cgatccgcat gctgcgcgag tacaccgagg
    10081 tcatgtatgt ccccgcctta cagcgcgccg agcgtctcag cgcggacgac ttcaccgtcg
    10141 cgcggcggct gaacagttgg aagcaggccc tcagccgcga atggcaccag gtgcgcatcg
    10201 aggaggtcga cgccaacgct caggaacgcc tcaaggtcgg cgataagctg cccattcacg
    10261 cccgcgttca cctgggcacc attcggccgg aaaacgtggc ggtcgaggtc tacgccggtc
    10321 cggttgatag taccggcggc atctcgcacg gccgccccgc gcccatgcag cacaccaagg
    10381 agaacggcaa cggtgttcac gagtttgacg gcgtgctgcc ctgtacggtc agcggtcagc
    10441 atggcttcgc catccgcgtc gtgccggcgc acgaagattt ggccgaccgg tacgagcagg
    10501 ggctgatcgt ctggggatag cgtcgaagcc gcagcctacc ggacttcgtc gatcgcggcc
    10561 ttcaatgcgg gtaggccgcg ttcgatggtc ttgcgatcca cgcagaagct gaggcgcatg
    10621 tagccggggc gtccgaagcc gcggcccggg accgccaaca cgcgatgctt caccaaaacc
    10681 gcgatgaact cggcgtcgtc ggcgatgggc gtcttcggga acgcgtatag cgcgccgccg
    10741 ggcactttca ggtcgtagcc gtaagacaat agggcgttgc acagcaaatc gcggttctgg
    10801 cggtagaagt ccaggtcgca gcgagcatcg gcgcagcggg cgatcacgcg ctgcatgaat
    10861 gccgaagcgt tcacgaagcc cagcgtgcgg ttgagcatgg tcatggccgc cagcactttc
    10921 tcgcgctgtg gcacggagtg cggcacggcg atatagccga tgcgctcgcc ggcgatgctg
    10981 atgtcctttg aatagctgga tattaggatg gtgcgcgggt agtggcgcag cggcgtgggg
    11041 caggtgtcta cgtcgtagac gatgcggcgg taggggtcgt ccagcaggat gtagatgggg
    11101 cggtcggggc ggtcgtgtcg cgccagcagc gcgcccagac cctggcactt ggcctcggtg
    11161 taaatcgcgc cggtggggtt gttcggcgag ttgatgataa tggcccgggt gcgctcagtg
    11221 atggccgcct caatcttccc cagatcgggc tggaaatcgg cgtccgtcgg taccagtacc
    11281 acccgtccgc cggcctgctc cacgtaaaag cggtattccg ggaagaacgg cgtgagaatc
    11341 agaacttcgt ccccggggtt gcagagtgct cgcagcgtca cgttcatgcc gccggccgcc
    11401 ccgctggtca tgatcacatc cgcggcctca aacggcgcgc cgtattcggt ccgcaggaag
    11461 cgggccaccg ccgcccgggc ttcgtcgaag cccacgttag gcatgtagcg gtgcagggcc
    11521 ggctggtgct cctcggccgc ctctcgcaag gcgcggaaaa aagcgtccgg cggcgtggca
    11581 ttggggttgc cgagcgagaa gtcgaaaacg ttgtcctcgc cgaactcggc tttgaggcgg
    11641 cggcccttct cgaacatctc gcgaatgaaa gaggcggacc ccatatcggc agcgactttg
    11701 tcggcaatca cggacggcat ggctattcct ctgcttgcgg atggtcttgg tgcgttcgcg
    11761 ggccgttcta ctggaacgcc gtcgccagtc gtgcccgcgt ttggcgcctg agcaaggcac
    11821 ccgcgcggcg gggccgcgtg cgtctacata gccaggcgca ggctaaccaa ctcgcacctg
    11881 tttcgccagg cgctccgttg cacgatccgt gccgcgcggc gaacgttctg ctactactct
    11941 gattccggga tgacgctgaa atcgatgcga aatccgccct cagcgtccgg atcttcaacc
    12001 agcgtgaaga tcaactcgtc tccgcacgca tagtcccagc ccagcaggcg ggcgtccccg
    12061 agcgcggtgc caactgttgg cagggcggct ccggggttgc cgggtgggat attcgagaaa
    12121 tgtaccactg agtcgaaggc gtccgggttg aaattgggga gcgtctcagg ggcgtctacc
    12181 acttcggcgc gctcgatgaa agcgcgggtg cccaccgaga aattgcgggc gcaggtgacc
    12241 gttgcgggcg cactactcga gttggccgcc agaaccagtt gctgcatctg taccggcccc
    12301 ggcgggctgc ggtcccaggc gtcccacgac ccgtaactaa acgccgccgt atagggcgta
    12361 gtgttgatga ccctcaccgt gatactgccg ctccgctcgg ccgtctggtt gagcagcacc
    12421 ggtccgcagc ccggctgaag caccagcaga agggcaacgg tcgccagcac cgcggccggc
    12481 agcgagaata gcgccttaat tgccatgtga atatccttgc gcgggccttt gggccaggct
    12541 gtccgcctgt gcgccgttat cgtcgcccgg tcgccgcgaa tgttagcaac gcccacgtga
    12601 actgccaaat ccggcgcggg gcccgagttg acggacgggc tgcggagcga ataatgcggc
    12661 agggttgcgc gccggagctg gtggcgggcg cagttacagt gcagcgcgac gcgtgccgat
    12721 agacttatcg ggccgagtcc tgctatgccg gactcggaca aaggtggaac aagaatgcgc
    12781 atcgctgttc tactgaccgc tgtgggcgcc tggatgctct caggcgcgag ctgtcggcct
    12841 ctcctcgata acacgtcgga tccagcggcc aaacccacca cgctgacgct gctgattcaa
    12901 tcgccatcca gcaacgcgac ggtggcgcag ggcgcatcgg tccccataac ctggaccgcg
    12961 tacaacgcca ccggtcagac ggccacgatt gacgtgtacg tcgaatcacg caccgatctc
    13021 actcagacaa cagtcgccga cgatatcagc ttgacggccg gcagtctgac ccaaacctcc
    13081 tattgggaca ccaccgggct gccgggcggc gaatacgtag tctacgccgc gattacagcc
    13141 ggggcggaca cgcagaccca gaaggccgca gggcgtataa cgatcgacga atttccggcg
    13201 ttcgagttca ccgagccggc agaagacgcc acgtacactc acggcagcga cgcgctcaag
    13261 atttcgtgga gcgcttcgga tccagagggc accggcacgg tgacggtggg gcttgacagc
    13321 gattcggatc ccgagaatgg gaacgagctt ttcatttacg aggggaccat tacggccgac
    13381 gagtccgaga gcagcttcga ctgggatggc caggacctgc tcggcgatga cgttgcgccc
    13441 ggcgtgtata acctgtttgc cttggtcggt gacgcggtca acccggagcg gcccgtgcaa
    13501 gccggccggc agatcaccgt cgtagagggg gcgccggacg agacctcggt gacactgggc
    13561 atcctggagc cgccctatcc ggcgacaccg gagaaccccg agccgcccaa agaccacgaa
    13621 ttcctggccg gcaccaccat gacgatcaaa tacggggtga acaaacaggt cgacgcgctg
    13681 atcgatctga agatcgatac cgacgacgta cacaccaacg gcaacgagct gacgattcgg
    13741 gctcagtggc tggtgcccgc cgacacggag agcgagactt ttacgtggga cggttcgctc
    13801 gcttccggag gagatgcccc tgacggcatt tatcgcctct tcatcatggc caatactggt
    13861 agcgcgacgc cggagtatgg ggatgacggc gaaggtctga tctaccggcg ctcctacgaa
    13921 aagcagcctc ttatagctct cgaaaaaccc gcgcatatga cgactttgca ggctggtcag
    13981 acgctgttga tcaaatggcg ctgcgtggat gacgagggga cggcaactat tcgtatcacg
    14041 attgacgacg acccgcatcc aacaggagac ccagacgacc cagatcccgc cgacacactg
    14101 cccgaaatcg ataccctggt aacggaccgg gcctgcagct ccgtcgaggt cgagaagact
    14161 tacatgtatc aggtacccgc gagcctgggc ccaggcgtgt attacgtctt cgcttatatc
    14221 cgcgacccgg aagcggcggg ccaggagcac tactcggtcg cgccggctcc attcgtgatc
    14281 agggatccga cccggtagat ggtctcgacg gtgcgagccg tcgccccgtt tgctgtacta
    14341 cgcactgcga ttttccaagg ccggccgcgc ggcgacgcgc ggtaccggct ggtgcttgta
    14401 tgacgccatc catacagcgc cctatcggag gtaactcgcc cgtgttcgca tccgccgccg
    14461 aggctcttaa gtatattaaa gacaaagact tagcaatggt cgacctgaag atcaccggcg
    14521 tcgccggcca gtggcttcac atctcaatcc ccgcgcgtca attttcgcag cggcacctgg
    14581 aagaaggcgt cggctatgac ggctcctccg gggccggctt ctcacgcctc gagagcggtg
    14641 acgtcgtcgc gcgccccctg ccggagaccg cgttcatcga tccgttcatg gaacgcccga
    14701 cgctgagctt cttgtgcgag acggtcagcg cggacaccag gattcccttt cccggcgacc
    14761 cgcggagcct gcggatcgaa cccagagcct ggcgatgact cccgtacgaa agcgtagaag
    14821 agcgggtcgg aatacagaac ggtgcgcgag ggttcgagga tgtgttcggg cgtgtggcca
    14881 aacggagcgc tcgagaagcc cgccaggaca gggccgcccc ggccatcgaa ggtataggcg
    14941 gccggatacc cgttgtatat cctgaacccc tccacgacgc tggccgccgt ctcgccgctg
    15001 tgaaaccaga agctgcggcc ctcataggca cagttgatgc ggcagtttgc gggatcgccg
    15061 gaagccgagc gcagggtaac agccttgccg tggaaatcca ggtcgtagtt gcccggccca
    15121 gtgtacgtac cgtcggcgac acgaatctcg tcgccgtcgg acgcggcgtc gagggcggcc
    15181 tggattgccg gatattgcga tggcacatac aggatggaac ccagggaatg ccgggcggcc
    15241 aggcccgact gcggcggctc gcccgcatcg ccagaggccg cggcgcggcc cggcaggacg
    15301 aagaccgccg ccgcgagcgt cgcgcagcaa atcgacacaa atttctgacg tgacaccttt
    15361 tcctccttac ctgtcaagac gccggccgaa gctgaccaga ttggtcagtc ccctgcaatg
    15421 accggctcca atttgaccac ggagtcaccg cccgtctggt ccggtgccct ccgcggatca
    15481 ctcaatggtg cggcctcatt ccaattgagc ggtttggtcc agtccacgcc aaatgtcgct
    15541 tgttgtgtga ttcggccgtt cgatcattat cggacaccgg cattgccnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tcgcttgttg tgtgattcgg ccgttcgatc
    15721 attatcggac accggcattg ccgccgcacc gtctcgggat ccgcgcactt cggcccgtgt
    15781 gtaccactgc ttgaaagcgc agcttcaacc agtgcgagaa ccgtgataag gtgatagagt
    15841 gataaagggg cgatccgcgg ccccccttat caccttatca ctttatcact accctcagga
    15901 tacgcgcact tccgcttcag acttggcttc ctcgcgctca ggataggcca cacgcgagtg
    15961 ataaatggcg cagagactct tgaccacgct gcgctggatc gtctccagtt cgcggaaagt
    16021 cagatcgcat tcgtccagtt gcccgtccat cagccgccgc tgcactatct ttgaaaccgt
    16081 atcctcaatg cggctggggg taggttcgga catagcccgc accgcccctt caaccgcatc
    16141 gcacagcatg accacggccg tctcgcgcga ctgcgggcgc gggccggagt agcggaagtt
    16201 gacttccgac acctccggat cgcccggctt gcgctgcaag ttggcggcgt ggtagaagaa
    16261 ttcgaccagc gtggtgccgt ggtgctcgac gatgaacggg tgcagggcgg gcggcagccg
    16321 gtactcgcgg gccatctcga tgccgtcttt gacgtgcccg gtgatgatta aatgactcat
    16381 agcgggcgat aggcgctcgt gccggctgtc tccgaccgcc tggttctccg cgaaatactc
    16441 cggcttgttg atcttgccaa tgtcgtggta atacgcgccg atgcgggcca gcagaccctg
    16501 cgccccgatc gcttcggccg cgttctccgc cagcgtgcca accagcagac tgtggttgta
    16561 cgtgccgggc gcttcggccg ccagcaggcg cagcagcggc ttgctggcat cgccccactc
    16621 cagcagcgac atgttcgtgc tgatcttgaa gacccgctcg atacccggca ggatgccttc
    16681 aatgatgaag gccgccgcca ttgtcgtgca ggccgccggc agagcctccg ccataaacgc
    16741 gaacctgagc gtctgaccgt cgatgagccc ggcggccaag gtcgtgaaca gtgcgaacaa
    16801 ggtcgcggcg gcgccgaccg ccacgatccc gccgcggttg cggatattgt tcagcccgac
    16861 gagaaacgtg ccggaaacga ccatcaggat gaggaagaag cccatgttct gccgcgtggc
    16921 cagcgtaatc agcaccgcca gacctgccgc catcgccggt ccgcaatagc gggcgtagac
    16981 gatgcccaaa atgccggcgg cgaaggcctg cacgcccacc gcgtaaaacg ggttgccggt
    17041 agccacgaag acagcccgcg caatggccag caccgccaac atcactaccg tcgtcgtcag
    17101 ttgccgggcc ggatcatcgc gcggcttgcg ccgtgggcgg cgcaagtagt aggtcagacc
    17161 gagcggaatc agaaaagcca gaaagccgta gccatatacc gccaggcgct cggaagcccg
    17221 ggctgcggcc acctcctggt gttcctcgat taagcgctga taggcgtccg gctggtggcg
    17281 ctgcagcgcc gccaattcct cttccgcggg cagctcacgc cgcagccatt tgcggtattc
    17341 ctcgtgttcg gccttgagca actcgtgctc tttcttggaa atctgtccgg catctgtcag
    17401 cagttgctcg cgcgcgtaaa cctcgtgctg tttggggacc gcggcccagg cctcctgcgc
    17461 ggcgcgactg gtcagatcag ccgcgtagcg gtagatggcc cgcaccgggc tatctgcgcc
    17521 ggccgctcct ggcgcggcag cgtcgggttc gcgccgcagc attccggcaa tcgagttctt
    17581 catcgacgcc tgcagcgggg cggcgaacgc ctgcgaagcg gccgcagcca ccagatcgac
    17641 cgcatccggc tgggcggtcg tgatgagctt cgaaaccggt aactcgcgtt ccacggcgtc
    17701 tttgccggcc tcggggggct cgatcagctt ggcccggtcc gcagttcgcc gagcggcggc
    17761 ctcggcagcc tcgaccagga gcggggtctg gctcttgagg ctggaaacgg ctcgttcgac
    17821 cgcgctctgg aagcgtgcca cttcgcgctg ttgttcgccg gcatcggcca ctttggcgat
    17881 tcgcaataac tcgtcgcggc ccgcttcgtc cagaatgatg cgggtgcggc gggcttcttc
    17941 ttgtaagcga gccgggtcgt cggcgtgcac cttcgcgagc tgcagggcgt tcgtcaggcg
    18001 gccggcgatg tccttgagca aggacacgtc cagctcgtag taattcgggc tgttgtcgcg
    18061 cgcccgcccg cgcatctcga tggtacgggt ggtgtcctcc agcctgaatt caacccgcgc
    18121 cgccagcgca cgtgagactg tttgcccttc gcgtatccgg aacaccgggc cgccggcgtt
    18181 caccaggtaa gccgccaaaa tcgcgaaccc caccgccagg ccgatcggcg ccaggctaac
    18241 ctgcgccggc aggcgacgat accaactcca gccgcgctcg gccttggaac ggcggatttc
    18301 tcggcggcgg gcggatgttc tgccaaacgg ccacataaga cgtaaccaaa tcagtgcggg
    18361 ctactgcttc gtctgttcgc cagccgggct tgaactcggt aagccgctgt ccggggccgg
    18421 ctccgtcggg gggagagtac ggcggcgatt atcagaccgt gtcggacgcc cggcagtcag
    18481 cggctcatca gcgtaacgcg tcacgacggc ctgcaccagc ttatggcgaa ttatatcgac
    18541 ttccgtcagc cgtagaatag cgatgccttc cacgccgcgc agccggctaa ccgcgtccac
    18601 cagtccggat gcctgcccag gctccaggtc gatctggctg tcgtcgccgg tcacgatcat
    18661 cttgctgtgg tgccccaggc gcgtcaggaa catcaacatc tgggagggcg tgcagttctg
    18721 cgcttcgtcg agaatgatta ccgcattatt gagcgttcga ccgcgcatgt aagccagcgg
    18781 aatcacctcg attatgtcgt ttaccatgaa ccgcttgagc tggtcgaacg tcatcatgtc
    18841 gtgcatggcg tcgaacagcg gccgcaggta ggggttcacc ttctgctgca ggtcgccggg
    18901 cagaaagccg agcttctcgc cggcttccac cgccgggcga acgagaacga tccggttggt
    18961 atgtccggac ttgagcaggc ttaccgcgac ggcgacggcc aggtaggtct tgccggtgcc
    19021 ggccggtccg gagcagaaca ccatgtcata gtcgagcatg gcgcggacgt agcggcgctg
    19081 gccctcggtg cgcggggcaa tgacgacgtg cggcgtgaat accgccagcg cctcgccgga
    19141 cgggcgctcc tgggcgccaa cgacgtcggc cagggtttgc tcgagggcac gttcgtcgat
    19201 gtactcgcgg ccgcggagca gttcctgcag gcgttcaacg acggccgccg cgcgtgcgac
    19261 gacggtgggt tcgccgacga tgcgtacggt gttattgcgg gcctggagct ggacgtcgag
    19321 cgtttggcgg atgcggcgga gataggcgtc gccgggcccg aacagggcag cccgcttatg
    19381 cggctccttc aaggtcagag ataattccat ctactatagg ttatccggcc ttggagcggc
    19441 tggccatggg gttgagagcc gctgactgtt ggcgatttgc ggagccgggg cgggccactc
    19501 aggaattgaa acggaaccgg ccggcttcaa ttcggccggc cgtaccttgc tcgagggcgt
    19561 tggccgaacc cgccgtccgg cttcaaccgg cgaagcccgg ccttcgccct cttgcacttt
    19621 gtgcgcgtgg cgacctggcc tagcgccgcc gcaccagcat caaggcggcc atcgccagca
    19681 gtcccagtga ggcgggctca gggacccagt tggcttgcat cgcatcgaaa atgaggcccc
    19741 agcccattcc ggcgttcgta gtcatgacga ttgtggaaat cggcgtgtcg ctgtgccagc
    19801 cttgccagcc ccatgtcccc ggcacaacgg tcagcgggag cgacccgatc accgctccgg
    19861 atgcatcgct gaacgtgatt gtgccgtctt ctgcggccgc gttcgtcgtg atataggttc
    19921 cgaagtcggt tacgggcacc gtgaactgaa gcaccgtatc gccggccgtt gtgccgccca
    19981 tccagttgcc aaagcgcggc atcacggtac cgccggggcc ttgccagtaa ttggcgatga
    20041 cgaccgtgtg cgagagggaa tcgtccatcg agcatcggcc acggaagatt tcgaccgggg
    20101 acggatgccc gccgggtgaa agcacgtctt caaaaccttc tgtggaatag ccggtgaact
    20161 cgggaacggt aacgatatcc gcccacgcca agcttgccgc gcatagcagc gaagcagtca
    20221 caaacacata cctcaaactc attcctcgac ctcctatgtt ctcctctgtc ggtcttttcc
    20281 tggccgcatt cacagacgag ctgtcggctc caaaataaca tagagggcgg attccatgca
    20341 agcttctttt tggaaagctg aaaaaatttc cccgtatatc cgataactag ccgtgaccaa
    20401 aacatccaca acttatcggc acatgatcaa gcccagcgta tacatgagtg cctgagcacc
    20461 tggccccggc gctacttcaa accgcgtttt tgtgcgggcc gcttgatcac ctgatcacct
    20521 gccgatcccg ccaggccgca aacgcgcgct cctcgccatt tctaacttga gctttgctct
    20581 tggaaccgaa actcgaaacc cgaactacca taactccggt agtggagaat aggcgatgct
    20641 tgcgagccaa cacgcgtttt tcggcaacat cctcgactac gccggcatgt ttccgccggc
    20701 gcaactgccg ctggacgcct caatccgcaa ctacctgcaa taccagtccg agccggaatc
    20761 ctggctgctg gcgcgcttca tttgcccagc cggccggctg cacgacctgc ggccatacgt
    20821 caccaaacat gtcagcggcc gatcgccact gcgaatatcc gtgctcggta gcggcggcga
    20881 tacggcggcg acttttctgg acaatttgaa tagcgatttg gaggccacgg cggacttccg
    20941 gcaggagttc ggccccgcgg tggcggtaga gtgctttgag gcgcgccttc cggccggaga
    21001 tttgctggag cccgctgact tgctgacgat gatctccgct gccttcgccg cgcagggatt
    21061 gacggcgctg ccgcgcttct ttgagctgcc ggcgactgcc aactggcccc gcatcgcaga
    21121 caccttggcc acacttcatc acgccgcttc gcccgaaggg cagggcgtgg ccggcggacg
    21181 tgccttgaat gggctgaagc tgcgctgtgg cggaagcgag ccggccgcca ttccgtcagc
    21241 cgcccaagtc gcgactgcca ttggagcctg cctgtccgcc aacgttccgc tgaagttcac
    21301 cgccggtcta catcagcccg tgcggtgcgt ggccgcggcg ctcggaacgc gcgtgcacgg
    21361 ctttctgaat ctgttcgcgg ccggcatcct cggcgcttcg gcggagctgg agtacgacga
    21421 cctggtaacg ataattgagg aagaggacgt tactcagttc agcttctccg atgatctctt
    21481 tgcgtggaac ggcgcgaagg ccacagtgag cgaaatcgcc cacgcgcgtc accgaaatgt
    21541 catcagtttc ggaagttgca gttttgacga accgcgcgac ggattgcgcg aactgggata
    21601 cctctaacaa tggctgagaa catcatgcgt tcgccgcgca gtgaacagcg ttcgtttatt
    21661 gagacggatc cggagtcgca ttttccaatt cagaatttgc cgtatggcgt tttcaaaaga
    21721 cccggtgaag aaccgcgcgt aggggtggcg atcggcgata tggtcctgga cttggcagca
    21781 cttgaagagc gagggttctt cgaagccgct gcactcggtg cgacgcctgt cttccgccgt
    21841 ccgtcgctga attacttcat gtcattgggg cggcccgcct ggcaacgggt gcgggcgatc
    21901 atcagccggc tcctgagcgc agacgagccc agattgcgcg atgatcccga attgcgccgc
    21961 gccgtgctaa tgccgcaggc gagcgttgaa atgtgcctgc ccgccgaaat cggcgactac
    22021 actgatttct actcctcgct cgagcacgcc atgaacgtgg gcaccatgtt ccggggcccg
    22081 gaaaacgcgc tgccgccaaa ctaccgctgg atgccgatcg cctaccacgg ccgcgccagc
    22141 tcgatcgtcc tcagcggcgc cgatctgcac cgcccgcgcg gccagacgcg gcgcgaggcg
    22201 ggggccgcgc cggagttcgg gtccagccgg cgggtcgact ttgagttgga gatgggcttt
    22261 tttgtcgggc cgggcaacga actgggctgc ccgattcccg ccgaccaggc cgccgagcac
    22321 atcttcggcc tggtcctggt aaacgactgg agcgcgcgcg atatccagca atgggaatat
    22381 gtccccctgg ggccgtttct tgccaagaac ttcggcacat ccatttcacc ctgggtagtc
    22441 acgctcgatg ccctcgagcc gttccgctgc gccggtccag agcaagaccc gcccccgctg
    22501 ccctatctcc gcactgaggg caagcaggcc tttgatatcc acctggaggt aacgctacag
    22561 cccgcggcca gcccacttcc gcacgtaatc tgccgctcaa actacaagta cctctattgg
    22621 aacatgtgcc agcaactggc gcaccacact atcaccggct gcaacctccg cccgggcgac
    22681 ttgctggcct ccggcacgat cagcggcccg gccccgggtt cctacggcag cctcctggaa
    22741 ctagcctggc gcggggaacg gccgctacaa ttgcccaatg gccaaaggcg aagcttcatc
    22801 gaggacgggg accgcatcgt aatgaccgcc tggtgccagg gcgacggcta ccgcgtaggc
    22861 tttggcgaag tgacggggcg aattctcccg cctcttgatc cctgacgatc ccttgatccc
    22921 tagctatttg ctagtccaag ttgaatcccc cgcgcgcgtc acccatgacg tgtgatagtc
    22981 cttatcttcc aggccggccg ccaccgtcgt cagccgcaac ggctcatgcg tgtcgcacat
    23041 aactgccaac tcgttcgtcc gttcggtgcc gatggatttc tcgtacgttc ccggatgtgg
    23101 cccgtgcgtt acgccctgcg ggtgcaggct gattgactca gggcgaatac ccttgcgaga
    23161 cgtgaagtcg ccggccacgt aatacagcac ctcgtccata tccacgctcg aatgcgcgta
    23221 cgggcagggg atcgccttct cgtggtaatc aaccttgcgc ggcacgaacg aacacaccac
    23281 gaaaccttcg cctgaaaaag tcgtgtgaat cgtgggcggc aggtgaacca ggcccgtctt
    23341 cggctggtaa tcgtggatgc tgaacgcgac cgggtagagc acgccgtccc agccgactac
    23401 atcgagagga aagtgcttgt accggtggac tgtcagctcg tcgcttgcct tcacgaccaa
    23461 ctcgaagggc ccgctgcgct gctccggctc aactgcgagt agttcctgag gcagccggaa
    23521 atcgcggtgt gagtacggcg cgtaatcggt caattgcccc cagcggttgc ggtaatcact
    23581 gggaattccc agccgcggcc ggccctcaaa aaacagtagc gtgccgcgat cgcccgccag
    23641 acacaggcgg tagggtgtgg acttgggaat caaaatgtaa tcatgctcgc gaaacggcag
    23701 aacgccatac acgctgtcca catagccact gccctgcgtg acgaaaaaga gctcatcgcc
    23761 atcgccgtta gagaagtagc gctgcggcga ctcagtcggc ttgacgacgc ccagatgcag
    23821 gtgacgcgtg accatcagcg tacgccggcc ggtcagaaaa tccccgcccg gcgccagctc
    23881 cgccgtccga atatgccggc ggtgcagcgg ctgctctgcg accaactcca ccggacagaa
    23941 gcccggcaag cgcagcgcgg ccacctcaaa ctcgtccgtc ggcggcgttc gataatacaa
    24001 aattgaataa gggccgttga atccgtcgcg cgtaatcatc tgctccatca gcaaccggcc
    24061 gtcctcatca tacagtgccg tgtgatgttt agccggaacg cgacccagtg tgtgacggtg
    24121 caacatacga gtaacctttg ctctttgacc tcgccaaacc ccgtgggctt acatcgctgc
    24181 aatcactatt ctagcgcgaa ttcacgtcgc cggagccctc ccacacgccg gccagaaagg
    24241 cctgcagccg ggcaaagtac tcgtccgtag ccaggtacgg gccgcgagcg tgcatcaggt
    24301 tcggaaactc aatgatcgtc acttctcccg gcgcccgcga cagaatcttc cggatttgcc
    24361 cgggtttggc atactcatcg cgcccgtaaa ctacggcaca aattggctga tgcacacgca
    24421 caagctgctc ctcgattcgc agccgcggat cgaacacctt catgtagtgc tccacccgcc
    24481 cgccgatgag caaccacgcc cgccgcactt gtagccgcag aacgagcagc ccgtccagca
    24541 cgaccgcgtt gactgcctcc ggccgggccg cggcttgggc taccgtcggc agcgtcccga
    24601 ctgagattcc catcagggtc actttgtcgc ggccggtccg cgccagcgtc caatcgagca
    24661 cggcagtgtg atcggtaacc agcgtgttga acgatgggcg gcccgtgctg ccgccgaatc
    24721 cctggaagtc gtacatgacc atcgaccagc cgtccgcgta caggtgtttg gtcagcagca
    24781 agtagcaggg cagctcgccc accgcgccgt aggccagcag aacgaggccg cgttccggct
    24841 gcgcggggac gtaccagact tgcagtatct ggtcgtcagc agttttgacg aaggctctct
    24901 cgaacggtat ccccagttcc gccggatctt tcaccgtggg caaatcgggc acaccgaaat
    24961 caactttaag ctgttcgcac gtcaattccg gcgcgaacgc gacgtcattg gcgagcgata
    25021 ggaactgctt tctcggaatc agcgggatac agccggccgc aaaaagcaag agcccaagca
    25081 agagtgctgt caacagtttt gtcatgcaca ttgtccgcgg cgccgcaaaa gtgcggcctg
    25141 aatctcgttt gcgtcgtcgc cgtatctgcc accattgctc tcatccgccc gcgcgcgtgc
    25201 gcccattcga agactgtccc ttttggtaaa aaattacatt cgattctagc ggcggatggc
    25261 tggtcagctc gccaggtaag agcccacggc gcttagccgg aacgcgaccg gcaggcgcta
    25321 tgatccaaca tcgccagtgg cgtccgccag ggcgtgccga tgatccgctc caaatgtgca
    25381 gcgaggagtg atgtcctcgt aacgttgaca tcagatcacg tggacccgga gaactccgat
    25441 gagtcgtgag aaggtccatg cgagccgcta cggtttagac accatcgggc tcgctcatca
    25501 ggcaactgct ttttggaacc tcccgccggc cacgctgatc gtcgaggctt tgcggcggcg
    25561 cgaggccacg ctggccgaca atggggcgct cgtggcactg accggcatgc gcaccggccg
    25621 ctcgccgcgt gataaattcg tggtcaaaga gcccgaaacc gagtcgcagg tgaactgggg
    25681 caaggtaaac atcccaattc cgcccgaggg attcgccagt ctacgggaca aggtttacca
    25741 ccatctgtgc tatgaggacg tatttgttca agacctctac gccggggctc atccggcgta
    25801 tcgcttgaac gtgcgcgtga tcactgagct ggcgtggcac agcttgttcg cgcggcagct
    25861 cttcattcgg ccgccgcggg acgccacggc cgacttcaag cccgatttca cggtcgtctg
    25921 cgctcccaac tgtctggctg acccgcagcg cgaccggata aattccgaaa cgttcgtggt
    25981 gatcagcttc tcccgcaagc tggtgcttat tggcgggacg cgctacgcgg gcgaaatgaa
    26041 gaagtccata ttcacggtac tgaattacgt tctgccggcg gaaggtgtgc tctcgatgca
    26101 ctgcgccgcc aatagcgggc acgcgggcga taccgcgctc t
//
LOCUS       DAOSYS010000016        25864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1568_length_25864_cov_5.513542, whole genome shotgun sequence.
ACCESSION   DAOSYS010000016 DAOSYS010000000
VERSION     DAOSYS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 25864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25864
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1568_length_25864_cov_5.513542"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2991)
                     /locus_tag="PLU99_01925"
     CDS             complement(<1..2991)
                     /locus_tag="PLU99_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO15843.1"
                     /translation="MLGRLRVARIGDRADWQNGAHCGVRFRRLLTLGVGCALLAAPRQ
                     LLAFPPNEPPDSTTATVPANNEAMLLFSRVEQAIQQADWRLVLALRERLMGMGNELVI
                     VPPGRTYYPIWRQATKLFERLPPEGRDLYRQTYEAEAAARLREIVPGGDLSALRDLFR
                     TYAGLSIWPAIGAELTAQLLDQGQYGDAIETIQVLNAAQSQMPPERRMQLVAALGCLG
                     HWQAAREHLDRLSATEAMQQPPWPERLAALRAWVAEASTSAGQAANGAANLSPALAAG
                     LWWEALEPASDNGACEDDLGMAEAIALTRRLPLQTAVLEGDILVTRGRGAIWAFDALT
                     LRPQWHVRELAWAVTPGERFDSSLVPSGMILGLGGQVEGLSADANALLSDALRHALST
                     GNELVFTIEATASGSSEEDDFGVARFGGMDYAPPPNELVARDLATGNVVWRRGSDPAD
                     ALYGVSFQDVPIVVRDRLCVPLVRAGELHLCLLAPSDGATVAEVEVVGPPTYFTRTGG
                     RCQLVSDESTVFVATGNGVVAALSSGDLTWKWATTYPSTLAARRSAGWWPPPQPSREV
                     SIDRPLLTGELLVLAPADAASIFALERATGRQRWRIQRRTDVSLVGATNSGVLLAGNS
                     LTHVDANDGATVLWRTVPLEISGRPAVGEDRIFVPTRDGVVTVDSHTGKILSEPGQGE
                     ISEPNQCASTSPPSGGALSPGLFSRHLSAANLVVAADAVFAISPNCLVKFPDIAAARK
                     SCAELLAMDPGDERARYAEIWLELLSGDPAAALAKAEEFQPSDVALAAGREKVLTDIF
                     VSLSDSLSDHTQALALLRRATSLAQCEDVSARLALLIGRLLEQGGHWAEAAQQYQELL
                     AQCRHSMIPIAGEPQAAQAAWLQAASRLRMAARQIAPEVRDQLWQAWLDSGSPELLQR
                     LRLATEDLKWQEEIDRALLVSSLPLEIRARYATPDDPSLPHEKRRELHLARWELHVRL
                     GLLAEAEADQRFWAEQMISAGQSDA"
     gene            complement(3098..3370)
                     /locus_tag="PLU99_01930"
     CDS             complement(3098..3370)
                     /locus_tag="PLU99_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15844.1"
                     /translation="MSRFRNRLIRLTLGLASAGVVFGGGCSVGSVVQYFANFNYGESV
                     LNMTRPAYQFLTSGYQGPGINPDIDPACTYPPFCTNDPFAPQASFP"
     gene            complement(3385..3603)
                     /locus_tag="PLU99_01935"
     CDS             complement(3385..3603)
                     /locus_tag="PLU99_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15845.1"
                     /translation="MLRSRRFKWVALLATFSAGTMLQFSTGCNQFWTDVALTSFDFCS
                     VVNCTGGTYFNFCDPVVILADCPNATAP"
     gene            complement(3860..4003)
                     /locus_tag="PLU99_01940"
     CDS             complement(3860..4003)
                     /locus_tag="PLU99_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15846.1"
                     /translation="MKKALLVLMASGVLFANGCLNWQWWLGASQFTSNVTNILEDVGV
                     LPG"
     gene            complement(4211..5311)
                     /gene="mtnA"
                     /locus_tag="PLU99_01945"
     CDS             complement(4211..5311)
                     /gene="mtnA"
                     /locus_tag="PLU99_01945"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOO15847.1"
                     /translation="MVEVENQRKVLPPTLEWIGGEDGCLRLIDQTRLPARLELLDCTT
                     VDQVRRAIQTLQVRGAPAIGIAGAYGLCLGLRPYRRADRALFGQKLREVSEYLAASRP
                     TAVNLAWAIGVVKAAGEAAATAEAAWDAMLAEAHALARADMEACRKIGEAGAHLVPEG
                     GGVLTHCNAGWLATVRYGTALSLLYVAQERGRRFHVYADETRPLLQGARLTAFELQAA
                     GIDVTVLCDNAAASVMQSGKVQLVVVGADRIAANGDTANKIGTYGLAVLARHHGIPFY
                     VAAPLSTFDPSLKSGAEIPIEQRDEAEVRAYGTSEVVPPGVSCLNPAFDVTPAALITG
                     IICERGIAKPVPEDIAEFTRQGLASRRALHTL"
     gene            complement(5428..7095)
                     /gene="nusA"
                     /locus_tag="PLU99_01950"
     CDS             complement(5428..7095)
                     /gene="nusA"
                     /locus_tag="PLU99_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOO15848.1"
                     /translation="MSQDLLRIIDSICRDKNIDRDSFVADLENAMASAIRKAHDETAD
                     AQVQLDPLSGKITATVNGQPVDLQRELGRIAAQTAKQVIIQKTRERERTSIFEEFTER
                     RGTIVTGTVVREERGSFIVNLGRTEGFLPRSEQIPGETHQVGERIRVLILDVRDQPNQ
                     IKIILSRSHPEFIVRLFELEVPEVAERIIELKALAREAGYRTKVAVSSIDSKVDAVGA
                     CVGVRGSRIKNIVEELGGEKIDIVRWNESSQILIQNSLKPAEVQEIALCFELGKATVV
                     VAEDQLSLAIGKRGQNVRLAARLTGWDVDILTPAEYNKHLEALERVVKSVEGATDMIV
                     DQLVAMGVVSVGDLDEVGPEPLVNELQLEPALAERIVEAAGEEAIKQEEEKEAAEKAR
                     AARIAAGLPPEEEAVATAEPAAEAAEGTPGESGSSAEGEATLTAETGSQGPGEGSAFV
                     PEATGTSAEDKFSDDSSKQFRPSIDFVTRTHTSTELGDSPEVMAVTHESPPAEPPQDE
                     SLGEQFEELVDDLTPGAAEREQPLDAEIRREGRGESPAEEDVSPLGG"
     gene            complement(7378..8784)
                     /locus_tag="PLU99_01955"
     CDS             complement(7378..8784)
                     /locus_tag="PLU99_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15849.1"
                     /translation="MPREPLPLLFEDTPRGLSRDLPPAARRSTRNGLFLVGLIFLGTR
                     LVTLVGAYYGSHILLLIQEQLRPTFVRHVREWEQVQAGKAESAEQAQRVAAELRAAHE
                     LLDDFAPLCRYDANHYQDIVERGYEYLSDPDDPLKTRGGYNIAWFPLYPLICRPLAQV
                     MSTRAALVLVANAAALAAAMLLYLWIRQRASEPVALCSVALLLCMPWAAFFAFGYAES
                     LTLLLVVVALRLIDGRRWLPAAVVSGLATATRPTALAIAGVLVLAFWFNAEYRGGGRL
                     GLRARIGRLVPLALVSVSGIVAYAIFLTVRFGTPFVYSANFKYWVQDERRADWLGFLT
                     FSRVFEQFKYFGAFFLPYPRGPMLAANPLMWNVPIILFILFVSLAGMKRVPRSFRPLL
                     MLGPLIFLHAYLACGGAVFGIEPIGRYLAVAVPTFVVLAAWCVREWSWGARTALISFL
                     LLVQGALAFRYSMNEWCG"
     gene            complement(8891..9646)
                     /locus_tag="PLU99_01960"
     CDS             complement(8891..9646)
                     /locus_tag="PLU99_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOO15850.1"
                     /translation="MMEFFTIDERRYARFERLAREGRLVHAFSTRPHNMAPRLDDRQA
                     ERAAARRQMAVDLGLSPDRLTICEQVHDCRVAIVPVGHAGGRLPGTDAVVTVGAGLPL
                     MGFSADCPLILLYDPLRPALGVVHASWRCTVAQLTAQAVETMKREFGCDPANLLAGIG
                     PGAGPCCYEVQADVFEAARDLPDRERLFRRRDGRMYFDLWQANVRQLCDAGLRAEQIE
                     TAGICTMCRVDVFFSFRREGPGCGHFGLMAGMR"
     gene            complement(9643..10617)
                     /locus_tag="PLU99_01965"
     CDS             complement(9643..10617)
                     /locus_tag="PLU99_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO15851.1"
                     /translation="MEALVIRHGAPRAVQYVNNYAAPQPAPGEVLVRVRLAGICATDL
                     EILRGYMSFSGVPGHEFVGTVVGGSERLRGRRIVAEINCVCGSCDMCSCGLSGHCRRR
                     TALGIQGRDGAFAELVCVPERNCHVVPDQVSDEQAVFVEPLAAAIQVVEVQPVDRWMD
                     VVVIGSGRLGLLVAQVLKLHGCRLEVVGRNPRTLAFCERHGIQPIELTELVPKATRDV
                     VVECTGSPEGLKLAMQLCRPRGTIVLKSTYAAPPPIDLAPVVVNELRIVGSRCGPFPE
                     ALQLLQQRQVEVEEMISRVYPLKAGPQAFEAAASPQEIKVLLRPGGGA"
     gene            complement(10688..11536)
                     /locus_tag="PLU99_01970"
     CDS             complement(10688..11536)
                     /locus_tag="PLU99_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO15852.1"
                     /translation="MQARNRLRCCAAWAALPAVVLLLAGCSDAKINAVVVKIFEPRRT
                     PQQNLVTAFSSEDPDVRRDSLGKVAKSKLHDEDWAVKGYITIALLDTEPQARCVAIRA
                     LVRTEDPRAAETLLKLLNYREHPPQEVRPPDTLIRWDATLGLARLSERDAVPPELRES
                     VEQTLIERLRMDEDAQVLTAAARGLGYYPSEDAVRALIGGLNGTNFAAVHECEESLVR
                     LTGYTHNCDPLAWQDWLEGHRDDLFARAGHIPESRRPPYSNRFEKVGYDVKQFWRWLV
                     PARKEQ"
     gene            complement(11565..12389)
                     /gene="murI"
                     /locus_tag="PLU99_01975"
     CDS             complement(11565..12389)
                     /gene="murI"
                     /locus_tag="PLU99_01975"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOO15853.1"
                     /translation="MSDKPIAVFDSGLGGLSVVRHLRHRLPHEQLVYFGDTARVPYGS
                     KSRQTVITFALEAARFLLQFEPKLIIAACNTASSLAMNELRQALPVPVIGVVEPGAAT
                     AVRLAGGGPIAVIGTEATVGSGSYLAAVRALAPEQEVIQRACPLLVALVEEGREPSDN
                     IVRLVVQEYLTPLREQAIRVLILGCTHYPLLRQPIADCLGPEIEIVDSGRETSLAVEQ
                     YLRATHSLCPPGSAGTMRCYVSDNPERFKRVGSRFLEEDIEQVEFVEPERYISAGV"
     gene            12596..13615
                     /locus_tag="PLU99_01980"
     CDS             12596..13615
                     /locus_tag="PLU99_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOO15854.1"
                     /translation="MVIWAWVGFVILVILLLALDLGVLNRKAHVIRTREALLWTGFWV
                     VLALLFNSLVYLIYENHWLDIGLSAFAEPDGREAALKFFTGFLIEKSLGMDNIFVIAL
                     ILAYFRVPLMYQHRVLFWGILGAIVLRGAMILAGAALVHRFDWVIYVFGGLLIVTAVK
                     MLFSGEQEIEPDRNPLVRIARRFYPVTTRMEGQRFFVRENGRRVMTPLFLALLIVESS
                     DVLFAIDSIPAIFAITADPFIVFTSNVFAILGLRSLFFALASLMDKFRYLKFSLVFLL
                     AYVGVKMLVSGVYKIPIGVSLSIIVGLLAAGVLASILASRWGVAQPASLFGRAPAIPR
                     AEDSD"
     gene            complement(13687..14676)
                     /locus_tag="PLU99_01985"
     CDS             complement(13687..14676)
                     /locus_tag="PLU99_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOO15855.1"
                     /translation="MPTALEIARQVIADEAEALRKLAGTLDGGFDAILETILSASQII
                     VSGLGKSGNIAQKIAASLTSTGTPAVYMHPVEALHGDLGIVSERHCLIALSCSGNTDE
                     VVRFVAHFGRLGGPVIGITQNGQSRLAELSKHFILLPEVPEAGPLNLAPTTSCVMMLA
                     AGDALAMALLHRRGFRREDFAQFHPEGTLGRRLLLTAADLMHSGERLPLVRAEDSFGQ
                     LILEMTRKGLGLAIIVNQDGGLLGTFTDGDLRRVFERVEKPRELDARTAHARSRRASG
                     APPVPVSTIRPTHLAVDCLRIMSESQITSLVVSDPEGKPVGVLRLMDLLKAGL"
     gene            complement(14676..18380)
                     /locus_tag="PLU99_01990"
     CDS             complement(14676..18380)
                     /locus_tag="PLU99_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15856.1"
                     /translation="MSVISARFSPSRFRVEVCSRICLFAAVWLSLAVLAGGLIPLASA
                     SQSQPAAIPQEQPAAIPQTQSALPQGQPTIPPPLQGQPQVDRRLVPLEDIRPAALAAG
                     TAHDLNQQPEVRNLFEAASGLMRSGKYEAAVDRLNAALNRSSHNYYDLLYLMAAAKFR
                     LRADGEARVYASQALQAARETADAHYLLGEICRRQTQLEAAIGHYRAATLAADTEATN
                     PRVTAAWFRLAECLAENGYLTAAYEAYTRFDEALWDTQPAHCEAEEIAPLLTDRPQGA
                     IQERIDLLKRLGRHSDAVAVSRAAAARWPDSLAVRRLLVRTLLAAGQHEQALAVCRLQ
                     WTDGEGPSFPEPDTAPRSLPLLSDTVKALSAAGQLEAWTAELERQVSAGQSLELAAAV
                     ADRLSKAGEYAAAAELWNAIAAQQPQDAEPVWAAAIALRASGDVGAAAELLAQFVRSH
                     PDNNDVPWKRFIAWLERDEGSAAELIKYVEKQVAASGRDFATDFVLATLAARGGQDEL
                     AEQLFAAAAEARPEFALAQVAWAQSLVARYEWEPAKMHALAALQEAEQLGAAHFVLGA
                     ACAGLDQNDEAEKEYRAAVKDSPNDAEFLLALGHFYKRRDDLLAAQRYFQQALTVDRD
                     NGEALENLVESYLAGGKPGLARLQFRESEDADLAEPVLRRIRTTLGFSENPFGPEHLA
                     ELKRQYTADSQDIETGLKLAAGLLVRGRYDEAFPYAQELHARFPQDARPAVLLARLLA
                     RRLEFRASADVLVNLVARYPNRGEMLRLLAVARLADFQLDEARRIIRHLLDLAQTDAA
                     RTALRDQLLISFVEFSEFEAALQLLDEWIAAEPENATWWREKLRVLALAGREQEALEL
                     ASSRVQPLEDEFQQKLGEFKRLLDRYKQLPEENLPMRSRAEGMQAEIKRIQSKLQERR
                     AEFVQVGMQVQNYQAVEEQLRQWLRDQPGDSEITQWLILLLIQDDRPDEALSQLEAFA
                     ATGLAAEAALRGWRAEAEAAAGRTDKAVAELDALLEEPPVGLSARDRAEVWAKLITIL
                     IEAQRYDDALARCDEWLSELPAKEQAMRLVVERSRLAVLATAGRQEQYLTEAERLLEA
                     DPDDTGLNNDLGYSWVDAGINVERALGMIRQAVADEPLRAAYLDSLGWAYYKTGDFDR
                     ACEYLRRAARLREGQDSVIFDHLGDACFRLGDREAARQHWQKAAQMLKEEEAKGGMAR
                     PPDLATKIRAKLAALEDGKEPEVAPTAGKN"
     gene            complement(18594..20237)
                     /locus_tag="PLU99_01995"
     CDS             complement(18594..20237)
                     /locus_tag="PLU99_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO15857.1"
                     /translation="MLGLAAVLIVAAALRLPALASLPPGLNQDEAANAWNAWCVLKTG
                     QDQVGQSWPIFCFRALGEYRSALFLYVVMPFQAVWGLNIWTTRLPSAIGGVATVVLAW
                     WVGRRMWGPGVGAVTAALLAVDPTHLQMSRLGHEGSITPLLTLAPLALLIWAGFVGPG
                     FGRETDSTITSVSPLRALVAGLVIGICCYGYPAARLFIPAFVAVCVLATPGGRLRLAR
                     SRRGLLALAALCLGIAITFGPLVWQHLAHPEMINRRGEATWVWRADDPVPLRAAKVLE
                     RYVRHFGPDFLYINGDALATVWTTGTGYIPWPLLPLQIGGLVLVVRRWRHSAAARVLV
                     VGLLLFPLGDCFNSHVGDSLSGLRSSPGLVPLSMLAACGLVWLAGSGIHARQQAAVIA
                     VAAALGLHFVKFAQNYTARGRELYVYSATHGDLLAAHQFLTEHIKPGDKLVFSDSGMN
                     QPYVVWLVADQYDPRRWFAEPREMQPGGDWDRYTRFGDTYFLNESERAALVQSLEGGG
                     RPDHVFFAVRPGETAPGTQIGQVPGPDGRPALVVYEAWL"
     gene            20630..21001
                     /locus_tag="PLU99_02000"
     CDS             20630..21001
                     /locus_tag="PLU99_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOO15858.1"
                     /translation="MADARTTVAELRAALAAFVAQRDWQIYHDAKNLSGSILIEAAEL
                     LEHFQWVRNDELPALLQDPAKRAAIAEELADVTCYVLSLANVLGIDLSSAVLDKIAKN
                     ARKYPADKFRGRYHKPEAPQA"
     gene            21340..22374
                     /gene="fliM"
                     /locus_tag="PLU99_02005"
     CDS             21340..22374
                     /gene="fliM"
                     /locus_tag="PLU99_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOO15859.1"
                     /translation="MSEVLDQAEIDALLASVTMTESGGTSASAGKAAGLPRADGGPAR
                     SPPSNASTYDFKRPERVSKDHIRALGSIHEGFARNFGATLSGFLRTIIDVRVVGVEQL
                     TYSEFIHSLPNPTCFAILQAPPLEGQMCLELSPLIVFPIIDRLLGGSSAELYIPQRPL
                     TSIEWRLINRLVERALQHLSEVWRNLVDAHFEVVDVETNPQLVHIVAPSEVVVFITFE
                     IKMGSCAGTMSMCIPFNTIESVLSKLTTQSWFGYKSKGATDVQQQRLLHNLTRSLVDV
                     TAYLGQTRIRLSELRALRPGDMIVLDKPTDRELLLQIGGRNKFAGVCGTLRGRRAMRL
                     ARRAEIDEPL"
     gene            complement(22396..>23341)
                     /locus_tag="PLU99_02010"
     CDS             complement(22396..>23341)
                     /locus_tag="PLU99_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOO15860.1"
                     /translation="IVALRANDTSIRALPAAQEFVQMKSTHGGEESKHHRSRITATIK
                     ALVRTRVTAGLITILPIVTTLWLLRVVFGWFRDASFWLVSAVLFSSWGHHLLESTGVN
                     VSAVSATGTNPTTLPAAGFDALPRGMRWGISIFSVLLTFFLLYVIGMFTANAIGRRLL
                     ELFELILDRVPFVKTIYRTLKQVLGLFGADEKQNFQRVALVPFPNELTRTVGFITNTF
                     KDSLTGEELCSVFIATTPNPTTGFVFVLRRSDVIEMDWSVEDAIKMAVSAGILLPPHI
                     TMTTGPKLAAVSSGCPAAAKPLSEARANTASSRKVRLD"
     assembly_gap    23342..23441
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23679..24836
                     /gene="yedE"
                     /locus_tag="PLU99_02015"
     CDS             23679..24836
                     /gene="yedE"
                     /locus_tag="PLU99_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOO15861.1"
                     /translation="MTKATSFFARRSGVIVAGLVIGGLAATLQNADLFSGRSNPPNMG
                     VCVACFTRDIAGSLGLHRASAVQYLRPEIPAFCLGAFIAALAFGEFRPRTGSSPIVRF
                     ILGMFAMIGALVFLGCPWRAMLRLAGGDWNAILGLAGLVSGIGLGVLMLRGGFSLGRA
                     YPARAVAGLAMPLLMGVLLLLAIFYPVFGEAGQAALFRSAKGPGAMAAPIVVSILVGL
                     VIGFLAQRTRFCTVGAFRDLLLMRDFHLLSGIIAFLVAAFALNLVYGQFKPGFTLGLD
                     SDGKPIQQFAAHSTHWLNFAGMLLAGLSFTLAGGCPGRQVFLSGEGDGDAAIFVLGML
                     TGAAVSHNFNLVGASKFAPYVVVIGLVVVGAIGLAGREVFARKEAKVCQPV"
     gene            24821..25045
                     /locus_tag="PLU99_02020"
     CDS             24821..25045
                     /locus_tag="PLU99_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOO15862.1"
                     /translation="MSTSVDARGLSCPQPMFLTRKALLEAGSGEVVVLVDGADQVDNC
                     TRTAERLGWQATCEQKTGPEGRVFELTFRK"
     gene            25051..>25864
                     /locus_tag="PLU99_02025"
     CDS             25051..>25864
                     /locus_tag="PLU99_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO15863.1"
                     /translation="MNYLDNAATSWPKPDTVAEAMLRYLRDVGASPGRSAHRLAIQSE
                     RLRLEAREAVAELVGLRDPLRVVFTHNGTAAINLALFGLLEPGAHVVATGMEHNAVMR
                     PLRALERRGVSISLAACRPDGTMEPAAVAECLRPHTRLVVMNHASNVCGTVLPVRQVG
                     ALARGRGIPLLVDAAQTAGCWPIDMHADNIDLLAFTGHKAMLGPTGAGGLALREDFDI
                     SRLPALVHGGTGSRSEHEFQPDFLPDKYEAGTPNAVGLAGLTAGVRYVLKRGV"
BASE COUNT         4595 a         8903 c         7761 g         4505 t
ORIGIN      
        1 ggcgtcagat tgcccggccg aaatcatttg ctctgcccag aaccgctggt ccgcctcagc
       61 ctccgccaaa aggcccagcc gcacatggag ctcccaacgg gccagatgca gttcgcgccg
      121 cttttcgtga ggcaggctgg gatcatcggg ggtcgcgtag cgcgcccgaa tctccaacgg
      181 caaggatgac accaacagag cgcggtcgat ctcttcttgc cacttcaaat cttcggtggc
      241 cagtcgcagg cgctgcagca actcgggcga accgctatcc aaccaggcct gccagagctg
      301 atcccgcact tcgggcgcga tctgccgggc cgccatccgc agccgggaag cggcctgtag
      361 ccacgccgct tgcgcggcct gcggctcgcc cgcgatcgga atcattgaat gccggcattg
      421 cgcgagtaac tcctgatatt gctgggctgc ttcggcccaa tggccgccct gctccagcaa
      481 cctgccaatc aacaatgcca gccgggctga aacgtcttcg cactgtgcga ggctggtggc
      541 gcgccgcagc agcgccaaag cctgcgtgtg gtcgctcagg ctgtcggaca agctgacgaa
      601 gatatcggtc aaaactttct cacgacccgc cgcgagtgca acatcactcg gctgaaactc
      661 ctccgccttg gccaatgcgg cggccgggtc gccgctcaaa agttccagcc aaatttccgc
      721 gtagcgcgcc cgctcgtcgc ccggatccat cgccagcagc tctgcgcagc tcttcctggc
      781 ggcggcgatg tccggaaact tgaccaggca gttcggcgaa atcgcaaata ccgcatcggc
      841 cgccacgacc aagttggcgg ccgacaggtg ccgcgaaaat agtccgggcg acagcgctcc
      901 acccgacggc ggcgacgtgc tggcgcattg gtttggttcg ctaatctccc cctgccccgg
      961 ttcgctgaga atcttgcccg tgtggctgtc caccgtaacg acgccatcgc gcgtcggaac
     1021 gaaaatccgg tcttcgccaa ctgccggacg gccgctgatt tccagcggga cggtccgcca
     1081 gagaaccgtg gcgccgtcgt tcgcatccac gtgggtcagg ctgttgccgg ccagaagtac
     1141 acccgaattc gtcgccccca ccaggctgac atccgtgcgg cgctgaatgc gccatcgctg
     1201 gcggcctgtg gcgcgctcca gcgcaaaaat cgacgccgcg tcggccggcg ccagcaccag
     1261 caattcgccc gtcagcaacg ggcgatcgat gctaacctca cgtgagggct gcggcggagg
     1321 ccaccaaccg gcgctgcggc gcgcggccaa ggtgctcggg taagtcgtcg cccacttcca
     1381 ggtcaaatct ccggaactca acgcggccac cacgccgttt ccggtcgcga cgaaaaccgt
     1441 cgattcatca ctgacaagct gacatcgtcc gcccgtgcgg gtgaagtacg tcggcggccc
     1501 cacgacctcg acttcggcca cggtcgctcc gtccgaaggc gcaagcagac acaggtgcag
     1561 ctcgcccgcc cgcaccagcg gaacgcacag ccggtcgcga accacaatgg gaacgtcctg
     1621 gaaggaaacg ccgtacagcg catccgcagg atcacttccg cgccgccaaa ccacattccc
     1681 ggtcgctaaa tcgcgcgcca ccagttcgtt gggcggcggc gcgtagtcca tccctccgaa
     1741 acgcgcgact ccgaagtcgt cctcctcgct cgatccggaa gcagtggctt cgatcgtgaa
     1801 taccaactcg ttgcccgttg atagcgcatg gcgcagcgcg tcggaaagca aggcgtttgc
     1861 gtcggccgac aatccctcca cctgcccacc cagacccaaa atcatgcccg atgggacaag
     1921 cgaagaatca aatcgctccc ccggcgtgac agcccaggcc aactctcgca catgccattg
     1981 cggcctgagc gtcagcgcgt cgaaggccca gatcgccccc cgcccgcgcg taaccagaat
     2041 gtcgccttcc aaaaccgcag tctgcaacgg caaccggcgc gtgagcgcga tggcctcagc
     2101 catgcccagg tcgtcttcgc aagccccgtt atcactggcc ggctcgaggg cctcccacca
     2161 cagcccggcg gccagcgccg ggctaagatt cgccgcgccg ttggctgcct gccctgcgct
     2221 tgtggaagct tccgcaaccc acgcgcgaag ggcagccagg cgctccggcc acggcggctg
     2281 ttgcatagcc tcagtggccg aaagcctgtc gaggtgctcc cgggccgcct gccagtgccc
     2341 caagcagccc aaagccgcca caagttgcat gcgcctctcg ggcggcatct gcgactgggc
     2401 cgcgttgagc acctgaatag tctcgatagc atccccgtat tgcccctgat cgagcaattg
     2461 ggccgtcagc tcggccccga tcgccggcca gatgctcaag cctgcgtagg tgcggaacaa
     2521 gtcccgcaat gccgacaggt ctccaccagg cacgatttcg cgcaggcggg ccgccgcctc
     2581 tgcctcgtac gtctggcggt agaggtcgcg gccttccggc ggcaggcgct caaacagttt
     2641 cgtcgcctgt ctccagatcg ggtagtaagt gcgacccgga gggacgatga ccaactcgtt
     2701 ccccatgccc atcagccgtt cgcgcagcgc aagaaccagc cgccaatccg cctgctgaat
     2761 ggcctgctcc acgcgtgaaa aaagcagcat ggcttcattg ttggcgggga cggtggccgt
     2821 cgtgctgtcg gggggctcgt taggaggaaa tgccagcagt tggcgtgggg ccgcgagcag
     2881 cgcacatcca acgccaagcg tcagcagccg gcggaaccga actccgcaat gcgcaccatt
     2941 ctgccaatcg gcgcgatcgc cgatcctggc gactcttaat ctgccgagca tgtgcgaatc
     3001 gtaacggcga cccacggaaa gaccatgggc caactaggcc gcggctcgcg cagactgccg
     3061 tccctgaggc atgccggctt gcggcgtcct cgtgagctta tgggaaggat gcttggggcg
     3121 cgaacgggtc gttggtgcaa aacggcggat acgtacaggc cgggtcaata tccgggttaa
     3181 tgcctggccc ttgatagccg gacgtcagga actggtacgc cggccgggtc atattcagca
     3241 ctgattcgcc ataattaaaa ttggcgaaat actggaccac tgaaccaacc gaacatcccc
     3301 caccaaagac gaccccagcc gaagccaagc cgagcgtcag gcgaatcagg cggtttctaa
     3361 aacgcgacat tgagagtctc acattcaggg ggccgtggcg ttgggacagt cagccagaat
     3421 tacaacgggg tcgcagaagt taaagtaagt tccgccggtg cagttcacaa ccgagcagaa
     3481 atcaaatgag gtcagagcta cgtcggtcca gaactggtta caaccggtgc taaattgcaa
     3541 catcgtgccc gcgctgaacg ttgccagcag ggccacccac ttaaagcgcc gcgaccttag
     3601 catttctcac tccggatttc ttctggacca acctccaaca gccgcagtat acgcacccgc
     3661 tggacggcca tcaagtctcg gacatggttg tcggccatca gcggcgggcc gctgctcgcg
     3721 cccccaaagc gcccccaaaa aaagccgaga cttgagcact ttgcggactc cgcagaatgc
     3781 ccaagccccg tgcgagaaac tcgccagcgc cttcccgaaa ggcgttcagc cttccggaga
     3841 aggcgcagac gccggcaagt tatccgggca gtacgccaac gtcttccaga atgttggtga
     3901 cgttggaggt gaactgcgaa gcgcccaacc accactgcca attgaggcag ccgttcgcaa
     3961 acagcacgcc cgacgccata aggaccaaca acgctttctt catctctttc tccttggaac
     4021 ttctttcaca cttccgctcg cggccgtgct cgctgacgcg aactagctgc gaagcgccac
     4081 tacatcgtcc gaaccataac cgcaccttat cggcggttgg cggatcggac ctttcgaaag
     4141 cagtatcaga agccggaaag cgcgctaccc cgatagggct gccggttctg accgcaccat
     4201 taaacagggg ttacagtgta tggagggcgc gacgcgatgc aaggccctgg cgagtaaatt
     4261 cggcgatatc ctccggaacc ggtttcgcga ttccgcgctc gcaaattatg cccgttatca
     4321 gcgctgcggg ggtcacatca aacgccgggt tcaaacaaga gacgccgggc ggcacgacct
     4381 ccgacgtccc gtaagcgcgc acttccgctt cgtcgcgctg ctcgatcgga atctccgcgc
     4441 cgcttttcag cgaggggtca aacgtgctca gcggcgcagc tacatagaat ggaattccgt
     4501 ggtggcgggc cagcaccgcc agcccataag tcccgatctt gtttgccgta tccccattcg
     4561 ccgcaatccg atccgctccg actacgacca actggacttt ccccgattgc attacgctcg
     4621 cggcggcgtt gtcgcacaga accgtaacgt caatgccggc cgcttgaagc tcgaaggccg
     4681 tcagccgcgc cccctgcaac aaaggccgtg tctcgtccgc gtaaacgtga aaacgccgcc
     4741 cacgttcctg cgcaacatag agcaacgaga gggctgttcc gtatcgcacc gtcgccagcc
     4801 agcccgcgtt gcagtgcgtc agaacgccgc cgccctccgg aaccaggtgt gccccggctt
     4861 caccgatctt gcggcaggcc tccatatcgg cgcgcgccag ggcgtgcgcc tccgccagca
     4921 tcgcgtccca cgccgcttca gcggtcgccg ccgcctcgcc ggccgctttg accactccta
     4981 tcgcccatgc caggttcacc gccgtaggac gcgacgccgc cagatattcg ctaacctcgc
     5041 gcaatttttg cccgaacaac gcccgatccg ctcgccggta cggacgcagg ccgaggcaca
     5101 agccgtaagc ccccgcgatc ccgattgccg gcgccccgcg aacctgcaac gtctgaattg
     5161 cccgtcgcac ctgatcgacc gtcgtgcagt cgagcaactc cagccgggct ggcagacgcg
     5221 tttgatcgat gagccgcagg cacccgtcct cccccccgat ccactctagc gtgggcggca
     5281 gcactttgcg ctggttttcg acctctacca atagagtacc ctcactccca cccttgcccg
     5341 actccgccgc tcgggctgaa cggaatgccg gcgcttggaa tgctccggca acgattcatg
     5401 gcatatagag cgactcgccc ggttcgccta gcccccgagc ggcgacacat cttcttcagc
     5461 cggactctcg ccgcggcctt cgcgcctgat ctcggcgtcc agcggctgct cgcgctcggc
     5521 cgctccggga gtcaagtcgt caacgagctc ctcaaactgc tcacccaggg actcgtcttg
     5581 cggcggctcc gctggaggag attcgtgagt gaccgccatc acttcgggcg agtcgcccaa
     5641 ttcggtgctg gtgtgagtgc gtgtaacaaa atcaatgcta ggccggaatt gcttgctact
     5701 atcgtccgaa aacttgtcct cggccgatgt ccccgtcgcc tcgggcacga aggccgaccc
     5761 ctctcccggt ccttgcgagc ccgtctcagc agtgagcgtc gcttctccct ccgcggacga
     5821 accggactcg cccggcgtcc cttccgcagc ctccgctgcc ggctcagcgg tagcaaccgc
     5881 ctcctcttcc ggcggcaggc cggcggcaat ccgcgccgcg cgagccttct cggccgcttc
     5941 cttctcctcc tcctgcttga tggcctcttc gcccgcggct tcgacgatgc gctctgccaa
     6001 ggcgggctca agctgtaact cgttgacgag cggttccggc ccgacttcgt ccaaatcgcc
     6061 gaccgaaacc acgcccattg cgaccagttg atcgacgatc atgtccgtgg cgccttccac
     6121 gctcttcact acgcgctcca gcgcctccag gtgcttgttg tattcggccg gtgtcagaat
     6181 atcgacgtcc cacccggtca ggcgggccgc caaccgcacg ttctgcccgc gtttcccgat
     6241 cgccagcgag agctgatcct ccgcaaccac caccgtcgcc ttgcccaact cgaagcacag
     6301 cgcgatttcc tgcacttcgg cgggcttgag cgagttctga atcaaaatct ggctggactc
     6361 attccagcgc acgatgtcga tcttttcccc gcccaactcc tccacaatat tcttgattcg
     6421 gctgccgcgc actcccacgc acgcgccgac ggcgtcgacc ttcgagtcaa tgctgctaac
     6481 ggcgaccttc gtgcggtacc cggcctcgcg cgccagggcc ttgagctcga taattcgttc
     6541 cgcgacctcc ggcacctcca gctcgaacag gcgcacgata aattccgggt gcgagcgcga
     6601 cagaatgatc ttgatctggt tgggctgatc ccgaacgtcg aggatcagga cgcggatgcg
     6661 ctcgcccacc tggtgcgttt cgccggggat ttgctcgctg cgcggcagaa agccctcggt
     6721 ccggcccaag ttgacgatga agctgccgcg ttcctcgcgg acgacggtgc cggtcacgat
     6781 ggtcccgcgg cgctccgtga attcctcaaa aatgctggta cgttcgcgtt cgcgggtctt
     6841 ctggatgatg acctgcttgg cggtctgggc ggcgatgcgg cccaactcgc gttgcaggtc
     6901 caccggctgg ccgttcacgg tggccgttat cttgccggac aacggatcta attgcacctg
     6961 cgcgtcggcc gtctcatcat gggcctttcg aatcgcgctg gccatggcgt tctcaaggtc
     7021 ggcgacgaag gaatcgcggt cgatgttctt gtcgcggcag atgctgtcga tgatgcggag
     7081 cagatcttgg ctcatgcgag tctcgctgcg ttcaaatccg gtcgtccggc aggcccaagc
     7141 ccgcctgtct tgcttactcc aacaaaaagg gccgctattc gcgacccaac cgagtccttt
     7201 cggactacgc ccaactttac ggccgcaagc cggcctcagc cgaccttctg ggccccgatc
     7261 gggccacctg cacctcaggc cctcacctca acatcaatag tctagccgat tcgggcccgc
     7321 ttgcccagta gaagtccgga aatcgtccgc ggcggctgcg acgggcgctg cgggctccta
     7381 cccgcaccac tcattcattg aataccggaa cgccagggcg ccctgtacca agagcaggaa
     7441 gctgatcagc gccgtgcgcg ccccccacga ccactcgcgc acgcaccacg ccgccagaac
     7501 cacgaacgtc gggacggcga ccgccaaata acggccaatc ggctcgatac cgaacacagc
     7561 cccgccgcac gccaggtacg cgtgcaggaa aatcagcggt cccagcatga gcagcggccg
     7621 aaagctgcgc ggaacgcgct tcatgcctgc caggctcacg aacaggatga acaagatgat
     7681 cggcacgttc cacatcagcg gattggccgc gagcatcggg ccgcgcggat agggcaggaa
     7741 aaaggcgccg aaatatttga actgctcaaa gacgcggctg aaagtcagaa aaccaagcca
     7801 atcggcccgc cgttcgtcct ggacccagta cttgaaattg gcgctgtaaa caaagggcgt
     7861 gccaaaacgg actgtcagaa atatcgcgta tgccacaatc ccgctgacgc tgaccagcgc
     7921 cagcggcacg aggcggccga tgcgagcccg cagacccagg cggcccccac cgcggtactc
     7981 ggcgttgaac cagaacgcca gcaccagcac gccggcgatg gccagcgccg tggggcgcgt
     8041 cgccgtggcc aggccgctga caaccgctgc cggcagccag cgccggccgt ctatcagacg
     8101 cagcgccacc actaccagca gcaaggtcag gctttcggcg tatccaaacg caaagaacgc
     8161 cgcccagggc atgcacaaaa gcagcgccac gctacacaat gcgaccggct cgctggcgcg
     8221 ctggcgaatc caaaggtaca gcagcatcgc cgccgccaac gccgccgcgt tcgccaccag
     8281 caccagcgcg gctcgcgtgc tcattacctg tgccagcgga cggcagatca gcgggtagag
     8341 cggaaaccag gcgatgttgt agccgcccct ggttttcagc ggatcatccg ggtcgctcag
     8401 atattcgtag ccgcgctcga caatgtcctg gtaatggttc gcgtcgtagc ggcacaacgg
     8461 ggcaaaatcg tccaacaact cgtgtgccgc ccgcagctca gcggcaacgc gctgcgcctg
     8521 ctccgcagat tcggcctttc ccgcctgcac ctgctcccac tcgcgaacgt ggcggacgaa
     8581 tgtgggacga agctgctcct ggatcagcag cagtatgtgg ctcccgtagt acgcgccgac
     8641 gagcgtcacc agccgcgtgc caaggaaaat caggccgacc agaaagagcc cgttccgggt
     8701 ggagcgccgc gcagccggcg gcagatcccg cgacaacccc cgcggggtat cctcgaacag
     8761 taacggtaga ggttccctgg gcacgctagt ttcccgagac tcccgtcaac agtgtaccgc
     8821 ctcctggaaa ttgtgcagcg agccgacttg cgtcctatcc gtcctattcg cctctaccgc
     8881 acggcggcca tcaccgcatc cccgccatca gcccaaagtg cccgcacccg ggcccctcgc
     8941 gccggaatga aaagaacaca tctacgcggc acatcgtgca aatgccggcc gtttcaatct
     9001 gctccgcacg caaacccgcg tcgcacaact gccgaacatt cgcctgccac aggtcaaaat
     9061 acatccggcc atcgcgccgt ctaaataggc gctctcgatc cggcaggtcg cgggctgcct
     9121 cgaatacgtc cgcctgcacc tcataacagc acggccccgc acccggcccg atgcccgcca
     9181 gcaggttcgc cgggtcgcag ccgaactcgc gtttcattgt ttctacggcc tgcgctgtaa
     9241 gctgcgcgac ggtacagcgc cagctcgcgt gcacgacgcc cagtgctggc cgcagcggat
     9301 cgtagagcaa aatcagcgga caatcggccg aaaagcccat gagcggcaac ccggcgccga
     9361 cggtgaccac tgcatcggta ccgggcagtc tcccgccggc gtgcccaacc ggtacgatcg
     9421 cgacgcggca gtcgtgcacc tgctcgcaga tggtcagccg gtcgggcgac agccccaggt
     9481 caaccgccat ttgtcgccgc gcggccgcgc gctcggcctg tctgtcgtcg aggcgcggcg
     9541 ccatattgtg aggccgggtg gagaaggcat gcaccagccg tccctcccgt gccaggcgtt
     9601 cgaaacgcgc gtagcgccgc tcgtcgatgg tgaagaattc catcatgcac cgccgcccgg
     9661 ccgcagcagc accttgatct cctgtgggct ggcggcggcc tcaaacgcct gcgggcccgc
     9721 tttcaacgga tagacccggc tgatcatctc ctcaacttcc acctgccgct gctggagaag
     9781 ttggagggct tccgggaaag gaccgcaacg gctgccgacg atgcgcagct cgttcacaac
     9841 cacgggcgcg aggtcgatcg gcgggggcgc ggcgtaggtg ctcttgagga cgattgtgcc
     9901 gcgcggccgg cagagctgca tggccagctt cagtccctcg ggtgagccgg tgcactcgac
     9961 cacgacgtcg cgcgtagcct tggggacgag ttccgtcagt tcaatcggct gtatgccgtg
    10021 acgttcgcaa aatgcgagtg tgcgcggatt acgcccgacc acttcgagcc ggcagccatg
    10081 cagcttgagc acctgcgcca ccagcagccc cagccgccct gagccgatca cgaccacgtc
    10141 catccagcga tcgaccggct ggacctcgac cacctgaatt gccgccgcta gaggctcaac
    10201 aaagacggct tgttcgtcgc tgacctggtc cggcacgacg tggcaattcc gctcgggcac
    10261 gcagaccagt tcggcaaagg ccccgtcgcg cccctgaatt cccaaagcgg tccgccgccg
    10321 gcagtgcccc gacaggccgc aactgcacat atcgcagctt ccgcagacgc agttgatctc
    10381 cgcgacgatg cgccggccgc gcaaccgctc agacccgcct accacggttc cgacgaattc
    10441 gtgccccggt acgcccgaga agctcatgta gccgcgcagg atttccaggt cggtcgcgca
    10501 gatccccgcc aggcgcacgc gcaccagcac ctcgcccggc gcgggctgcg gcgctgcata
    10561 gttgttcaca tactggacgg ctcgcggcgc cccatgacga atgactaacg cttccaagct
    10621 tcgtgcctcc cgacttcagc cacgggcgcg ctgatcggct agtggtctga ccgcgaaccc
    10681 gaactgctca ctgctctttg cgggccggta ctaaccagcg ccagaactgt ttcacgtcgt
    10741 agcccacttt ttcgaagcgg ttgctgtagg gcgggcgccg cgactcgggt atgtggccgg
    10801 cccgggcgaa caggtcgtcc cgatggcctt ctagccaatc ctgccacgcc agcggatcgc
    10861 agttgtgtgt gtaaccggtc agcctgacca gcgactcctc gcactcgtgg actgccgcga
    10921 agtttgttcc gttcaggccg ccgatcagcg cccgcaccgc gtcctcggac gggtaatacc
    10981 ccaacccgcg cgccgcggcc gtaagcacct gggcgtcttc gtccatgcgc aggcgttcga
    11041 tcagcgtttg ctcaaccgat tcgcgcaatt ccggcggcac cgcgtcccgt tcagacagcc
    11101 gcgccaggcc gagcgttgcg tcccagcgaa tcagcgtatc cggcgggcgg acttcctggg
    11161 gcgggtgctc gcggtagtta agcagcttca gcagggtttc ggccgcgcgc gggtcttcgg
    11221 tgcggaccaa ggcgcggatg gccacgcaac gggcctgcgg ctcagtgtcc agcagggcga
    11281 tcgtgatgta ccccttaacg gcccaatcct cgtcgtgcag cttgctcttg gccaccttgc
    11341 ccagcgagtc gcggcgcaca tcggggtctt cggaagaaaa ggcggtgact agattctgct
    11401 gcggcgtgcg gcgcggctcg aagatcttca ccaccacggc gttgatcttg gcgtcgctac
    11461 aaccggctag cagcagcacc accgccggca gcgccgccca ggcggcgcaa caccgcaggc
    11521 ggttgcgcgc ctgcattttc acactggcgg cccggcgatg gggttcacac gccggccgag
    11581 atgtatcttt ccggctcaac gaactcaacc tgctcgatat cctcctcgag gaagcgcgaa
    11641 ccgacgcgtt tgaagcgttc ggggttgtcg ctcacgtagc aacgcatggt acctgcgctt
    11701 cccggcgggc acaaggaatg cgtggcgcgc aggtactgtt cgaccgcgag cgaggtttcg
    11761 cgcccgctgt cgacgatctc aatctcgggg ccgagacaat cggcgatcgg ctggcgcagc
    11821 agcggataat gcgtgcagcc caggattagc acgcgaatgg cctgttcccg aagcggtgta
    11881 aggtactcct gcactacgag gcggacgatg ttgtcggacg gctcgcgtcc ctcctcgacg
    11941 agagccacca gcagcgggca ggcgcgttga atgacctcct gctcgggcgc gagggcgcgc
    12001 acggccgcca ggtaggagcc gctgcccacc gtggcttcgg tgccgatgac ggcgatgggc
    12061 cctccgccgg ccaggcggac ggcggtggcg gcgcccggtt cgaccacgcc gatgaccggc
    12121 acgggcagcg cctgccgcag ctcgttcatg gccagcgagc tagcggtgtt gcaggccgcg
    12181 atgatcagtt tgggttcgaa ttggagcagg aagcgggccg cttcgagggc gaaggttatg
    12241 acggtctgcc gcgatttgct gccgtacggg acgcgggcgg tgtcgccgaa gtacacaagc
    12301 tgctcgtgcg gcaggcggtg gcgcaggtgg cgcaccaccg aaagcccgcc gagtccggaa
    12361 tcgaacaccg cgataggctt gtcgctcatt ggtcctcctt gaccctctac acgaccggtt
    12421 agccaccagt cagtccgcag gataaagcaa gcgggcggga gttcaaggcg gggtttcggc
    12481 cgccggaagg ccggcgggaa cgcattttgc ggtaactcag gttctggcgc gcgctccgca
    12541 cgcccggcgc tctataatga gcactttttg aaacgcagcg gttttcggag caggcatggt
    12601 catctgggcc tgggtcggtt tcgtcatact cgtgatactt ctgctggcgc tcgacctggg
    12661 cgttctcaac cgtaaagcgc atgtgatccg cacgcgtgaa gcgctgctgt ggaccggctt
    12721 ctgggtggtt ctggcgctgc tgttcaacag tctggtttac ctgatctatg agaaccactg
    12781 gctcgacatc gggctgagcg cgtttgcgga gccggacggc cgcgaggcgg cgctgaagtt
    12841 cttcaccggt ttcctgatcg aaaagtcgct ggggatggac aacatcttcg tcatcgccct
    12901 gatcctggcg tatttccggg ttccgctgat gtaccagcac cgggtgctgt tctggggcat
    12961 tctgggcgcc atcgtcctgc gcggcgcgat gattttggcc ggcgccgccc tggtccaccg
    13021 cttcgactgg gtcatctacg tcttcggcgg gctgctaatc gtgaccgcgg tgaaaatgct
    13081 tttctcgggc gagcaggaaa tcgagcccga ccgcaacccc ttggtgcgaa tcgcgcggcg
    13141 cttctatccg gtcacgacac ggatggaagg ccaacgcttt ttcgtccggg agaacggccg
    13201 ccgggtcatg accccgcttt tcctggctct gctgatcgtc gaaagttcgg acgtgctgtt
    13261 cgcgatcgat tcgatacccg cgatttttgc cattacggcg gacccgttca tcgtctttac
    13321 gtcgaacgtg ttcgcgattc tggggctgcg ctcgctgttt ttcgcgctgg ccagcctgat
    13381 ggataaattc cgctatctga agttcagcct ggtattcctg ctggcgtatg tgggggtgaa
    13441 gatgctcgtt tcgggcgtct acaagattcc gatcggggtt tcgctgagca tcatcgttgg
    13501 gcttctggcg gcgggagttc ttgcgtcgat attggccagc aggtgggggg tggcgcagcc
    13561 cgcatccctg ttcggccggg ccccggcgat tccgcgcgcc gaagattcgg actgataaac
    13621 gtactggagg cgcgacgctc gggcggcgcg gacagcctgc tcgcagcgac cgcagggtca
    13681 aaccttctac aaccccgcct tcagcagatc catcagccgc aacaccccca ccggcttgcc
    13741 ttcggggtcg ctgacgacca ggctcgtgat ctggctctca ctcatgatcc gcaggcaatc
    13801 gaccgccagg tgcgttgggc gaatagtcga caccggcacc gggggcgcgc cgctggcacg
    13861 ccggctgcgg gcgtgggccg tccgcgcgtc cagctcgcgc ggcttctcca cgcgctcgaa
    13921 cacgcggcgc aagtcgccgt ccgtaaacgt gcccaacaaa ccgccgtcct ggttgacgat
    13981 gatcgccagc cccagcccct tgcgcgtcat ctccaggatc agttgcccaa atgaatcttc
    14041 cgcacgcacc agcggtaaac gctcgcccga gtgcatcaaa tccgccgccg tcagcaacaa
    14101 acgccgcccc agcgtcccct ccggatgaaa ctgggcgaaa tcctcgcgcc gaaacccgcg
    14161 ccggtgcagc aacgccatcg ccagcgcatc ccccgccgcc agcatcatca cgcaactggt
    14221 cgtcggggcc aggttcaacg gcccggcttc cggaacctcc ggcagcaaga tgaagtgctt
    14281 tgataattcg gccagccgcg attgcccgtt ctgcgtgatg ccgatcaccg gcccgcccag
    14341 tcgcccgaaa tgggccacga agcgcacgac ttcgtccgta ttgccgctgc aggagagagc
    14401 aatcaggcaa tgccgctcgc tgacgatgcc caggtcgccg tgcagcgcct cgaccgggtg
    14461 catgtacact gccggcgtcc cggtgctggt caatgatgcg gcgatcttct gggcgatgtt
    14521 gccgctcttg cccagcccgc tgacgatgat ctggctggca gacagaatgg tttcgaggat
    14581 ggcgtcgaaa ccgccgtcca gcgtgccggc cagtttgcgc aacgcctccg cctcatccgc
    14641 gatcacctgg cgggcgattt ccagggctgt gggcatcagt tctttcctgc ggtcggcgcc
    14701 acctccggct ctttcccgtc ttccaacgcg gccaacttcg cacgaatttt ggtcgcaagg
    14761 tccggcggac gcgccattcc gcctttggct tcctcttctt ttaacatctg cgcggctttc
    14821 tgccagtgct ggcgggcggc ctcgcgatcg cccaggcgga aacacgcatc acccaaatgg
    14881 tcgaagatga ccgagtcctg cccttcacgc agccgcgccg cacgccgcaa atactcgcaa
    14941 gcccggtcaa aatcgcccgt cttgtagtag gcccagccca aactgtctag gtacgccgcg
    15001 cgcaacggct cgtccgctac cgcctggcga atcatcccca gggcccgctc gacgttgatg
    15061 cccgcatcca cccacgaata acccaggtca ttgttcagcc cggtatcatc cgggtccgcc
    15121 tccagcaaac gctccgcctc ggtcaggtat tgttcctgtc gcccggccgt ggccagaacg
    15181 gccagccgcg agcgctcgac caccaaccgc atggcctgtt ctttggcggg cagctcgctc
    15241 agccattcgt cgcaacgggc cagcgcgtcg tcgtagcgtt gggcctcgat caatatcgtg
    15301 atcagtttcg cccagacttc cgcccgatcg cgggccgaca agccaaccgg cggttcctcc
    15361 agcagggcat ccaactcggc caccgcctta tccgtccgcc cggcggcggc ttcggcctcc
    15421 gcacgccagc cgcgcagggc cgcttccgcc gccagcccgg tggcagcgaa cgcctctagc
    15481 tgcgaaaggg cctcgtcggg gcggtcgtcc tggatgagca gcagaatcag ccactgcgta
    15541 atctcgctgt cgccgggctg gtcgcgcagc cattggcgca gttgctcttc gaccgcctgg
    15601 tagttctgca cctgcattcc aacctgcacg aattccgcac gccgttcctg cagcttgctt
    15661 tgaatgcgct tgatctcggc ctgcataccc tcggcccggg agcgcatcgg caaattctct
    15721 tccggcaact gcttgtaccg gtccagaaga cgtttgaact cccccagctt ctgctgaaac
    15781 tcgtcttcca acggctgcac gcgcgaactg gccagctcaa gcgcctcctg ctcacgaccc
    15841 gccaacgcca gcacacgcaa cttctcacgc caccaggtcg cgttttcggg ttcagccgcg
    15901 atccactcgt ccaatagctg caacgcagct tcaaactcgg agaactcgac gaacgagatc
    15961 agcagttgat cgcgcagggc cgtgcgcgcg gcgtccgtct gcgctaagtc cagcaaatgc
    16021 ctgataatcc ggcgggcctc atcaagctga aaatccgcca gccgcgccac cgccaatagc
    16081 cgcagcattt cgccccggtt cggataacgc gctaccaagt tgaccagcac gtcggccgaa
    16141 gcccgaaact ccagccttcg cgccagcagc cgcgccagga gcaccgccgg ccgcgcatcc
    16201 tgcgggaagc gggcgtgcag ctcttgggcg tacggaaaag cctcgtcgta gcggccgcgc
    16261 accagcaacc cggcggccag cttcagtccc gtctcgatgt cctgtgaatc ggctgtgtat
    16321 tgccgcttta actccgccag gtgttcaggc ccgaacggat tttcgctgaa gcccagcgtg
    16381 gtgcgaatgc ggcgcaggac aggttcggcc aggtcggcgt cttcgctctc gcggaattgg
    16441 agccgggcca gacccggctt gccgcccgcc agatagcttt cgaccaggtt ttccagggct
    16501 tcgccgttgt cgcggtcaac cgtgagggcc tgttggaagt agcgttgtgc cgccagcagg
    16561 tcgtcgcgcc gcttgtaaaa atgcccgagc gcgagcagaa attctgcgtc gttcggcgag
    16621 tccttgaccg ccgcgcggta ctccttctca gcttcatcgt tctggtcgag accggcgcac
    16681 gccgcgccga gcacaaagtg cgccgcgccc agttgctcag cttcctgcaa ggccgcgagg
    16741 gcatgcatct tggccggctc ccactcgtat ctggcaacca gcgattgcgc ccacgcgacc
    16801 tgcgccagag caaactccgg acgcgcctcg gccgctgcgg cgaacagttg ttcggccaac
    16861 tcgtcctgcc cgccccgcgc cgccagcgtc gccagcacga aatccgtcgc aaaatcccgg
    16921 ccagacgccg caacctgctt ttcgacgtac tttatgagct cagccgcgga accctcatca
    16981 cgctccagcc aggcgatgaa acgcttccac ggcacgtcgt tgttgtcagg atgcgagcgc
    17041 acgaactgcg ccagcaactc agccgccgcg cccacgtcgc cgctggcgcg tagcgcgatt
    17101 gccgccgccc acaccggctc tgcgtcctgc ggctgctggg ccgcgattgc gttccacaat
    17161 tcggccgccg cggcatactc gcccgctttt gaaaggcgat ccgcaacggc cgccgccaat
    17221 tcaaggctct gccctgcgga aacctgacgt tccaactccg ccgtccaggc ctcaagctga
    17281 ccggcggcac tgagggcctt caccgtgtcc gacaagagcg gcaacgaacg cggcgccgtg
    17341 tcgggctcgg gaaaagacgg gccctcgccg tccgtccatt gcaggcggca aaccgccaaa
    17401 gcctgctcgt gctgaccggc ggccagcagt gtccgcacca agaggcgcct caccgccagg
    17461 ctgtcaggcc aacgtgcagc ggcggcgcgc gataccgcaa ccgcgtctga atggcggcca
    17521 agccgcttca gcaggtcgat ccgctcctga atcgctccct gcggacggtc agtcaacagt
    17581 ggcgcgattt cctcggcctc gcagtgcgcg ggctgcgtgt cccagagcgc ttcgtcaaag
    17641 cgcgtgtacg cctcataagc ggccgtcaaa tagccgtttt ccgccaggca ttccgccagg
    17701 cggaaccagg ccgccgtgac gcgcgggttc gtggcctccg tatccgcggc cagtgtcgcc
    17761 gcccggtagt gtccaatcgc ggcctcgagt tgcgtttgac ggcggcagat ctcgcccagc
    17821 aagtagtggg cgtcggccgt ttcccgcgcg gcctgcaacg cctgcgacgc atacacgcgc
    17881 gcctcgccat cggcccggag ccggaacttg gccgccgcca tcagatagag caggtcgtag
    17941 tagttgtgtg aggagcggtt cagcgcggca ttgagccggt cgacggcggc ctcgtacttg
    18001 ccgctgcgca tcagccccga cgccgcctcg aacaggttcc gcacctcggg ctgctgattc
    18061 aggtcgtgcg ccgtccccgc ggccaaggcg gccgggcgaa tatcttccag tggcaccagc
    18121 cggcggtcga cctgaggctg cccctgcaag ggcggcggaa tcgtcggctg cccctgcggg
    18181 agagccgact gagtctgcgg aatcgcggca ggctgctcct gcggaatcgc ggccggctga
    18241 ctctgtgaag cggaagccag gggtatcagt ccgcctgcca gcacggctaa tgacagccag
    18301 accgctgcga acaggcagat ccgcgaacag acttccaccc gaaatcttga aggcgaaaag
    18361 cgcgctgaaa tcacggacat aagccctcga cagtgtagtt ccaggctccc gtcgcacgcg
    18421 cgggaaccgg gcgattcacg agcctttcct gtacccgaag cacgccagcg cgttgtagct
    18481 cgccgcgcgg cgaaagtcca gcagcccggg ccccacgcga ccagcgaacg cggcagccgg
    18541 ccgcaacacc gccactgcat gaagcagctt cacgcagtgc ggtccccgcg cctttacaac
    18601 catgcctcgt acacaaccag cgccggccgt ccatcgggcc cgggcacttg gccgatttgc
    18661 gtcccgggcg cggtttcgcc cggacgaact gcaaagaaca cgtggtcggg gcgaccgccg
    18721 ccttccagcg actgcaccaa cgctgcgcgc tcgctttcat ttagaaagta ggtatcgccg
    18781 aaacgcgtgt agcggtccca gtcgccgccg ggctgcattt cgcgcggctc tgcgaaccag
    18841 cgccgcggat cgtactgatc cgccaccagc cagacgacgt acggctggtt cataccgctg
    18901 tccgagaaga ctaatttgtc gccaggcttg atatgctcag ttaagaactg gtgcgcggcc
    18961 aacaggtcgc catgcgtggc ggagtacaca tacagctcgc gccctcgcgc agtgtaattc
    19021 tgtgcgaact tgacgaaatg gagcccgagc gccgcggcga ctgcgatcac cgccgcctgc
    19081 tgccgggcat gtattccgct gccggccagc cacaccagcc cgcaagccgc cagcatcgag
    19141 agcgggacga gaccgggcga gctgcgcagc ccgctcagac tgtcgcccac gtgcgaattg
    19201 aaacagtcgc caagcgggaa cagcagcaga ccgacgacca aaacgcgggc cgccgccgag
    19261 tggcgccaac gcctgaccac cagcactaat ccgccgatct gcaatggcag cagcggccag
    19321 ggaatgtaac ccgtgccggt cgtccagacg gtcgcgagcg catcgccgtt tatatacaga
    19381 aagtcagggc cgaagtgccg aacgtagcgc tccagaacct ttgcggcgcg gagcggaacc
    19441 gggtcgtcgg cgcgccacac ccaggtggcc tctccacgcc gattgatcat ctccggatgc
    19501 gccaaatgct gccataccag cggcccgaac gtgatcgcta tgcccaggca cagcgccgcg
    19561 agcgcaagca gcccacggcg gctgcgcgcc agtcgtagcc ggccgccagg agtcgccagc
    19621 acgcataccg ccacgaaggc cggaatgaaa agccgcgccg ccgggtagcc atagcagcag
    19681 attccaatta ccagcccggc gaccagggct cgaagcgggc tcacagatgt aattgtggaa
    19741 tcagtttcac gtccaaaacc cgggcccacg aaacccgccc aaatcagaag cgccagcggc
    19801 gccagcgtaa gcagcggcgt gatcgacccc tcgtgcccca gccggctcat ctgaaggtgc
    19861 gtcggatcca ccgccagcaa ggccgccgta accgctccca cccctggccc ccacatccgc
    19921 cggcccaccc accacgccag caccactgtc gccacgcccc cgattgcgga cggcagccgt
    19981 gtcgtccaga tattcaagcc ccacaccgcc tggaacggca tcaccacata aaggaacagc
    20041 gcgctgcgat actcgcccag cgcacggaag cagaaaatgg gccaactctg cccgacctga
    20101 tcttgccccg tcttcagaac gcaccacgcg ttccaggcgt tggccgcttc gtcctgattc
    20161 agccccggcg gcaacgaggc aagtgccggc agccgcaaag ccgcggccac gatcagtacc
    20221 gccgccaggc ccaacatctc caatccgttc ggccgcccag cccgtaccaa gggttgcgcg
    20281 gcctgtgcga aatccgctgc tgcaaggatg gaatgaggtg ccgggccgct ctcgggccgg
    20341 cgcatgccaa gcatggcgga atgctatccg ctgccggcaa gatcggtaag gccgcacggc
    20401 gccggtggtc gagcttcttt ttgggggtcc ctttttgacc gatctgaact agtgtgtaac
    20461 cggtttgtga caagtctgac ctcgccacga cggaccggtt ctgcctgcga cgcctgcggg
    20521 ccgcgtcggg caaacttcgg tcaggcacaa ttcggctggt tgcttgccct cgcatttccc
    20581 cttatcacaa gcgttaagcg gggtcgggtg cccgcgacgg gagtttgtga tggctgatgc
    20641 gcgcaccacc gtagccgaac tgcgagcggc gctggcggcg tttgttgccc agcgcgactg
    20701 gcagatttat cacgacgcga agaacctgtc gggctcgatt ctgattgagg ccgccgagct
    20761 actggagcat ttccaatggg tgcgcaacga cgagttaccg gcacttctgc aagatccggc
    20821 caaacgggcc gcgattgccg aagagctggc ggatgtgacc tgttacgtgc tctcgctggc
    20881 gaacgtgctg ggtatcgatc tttcgtcggc cgtgttggat aagattgcga agaacgctcg
    20941 caagtatccg gccgataagt ttcgcggtcg ctaccataaa ccggaggcgc cccaggcgtg
    21001 agccgccccg ctgtgggtcg ttctgggcgt agtatcgtgg ggcgaccggc gattttaaga
    21061 gaccgcgatg gcccggccga agcgtcgtga cccaatgtcg tccgagacgc tgatgctcgt
    21121 actggcggtc gtcttttgcg cgatcgtcgg cgggttgctg gtgctggtct atcgcttctc
    21181 cggggttgaa ttgctgtaag aaccgttgta acgcgtccgg taagcagagc cggagcgtgt
    21241 cgcctccgta gcgtaccgcg ccctgctcgc atcaagccgc gaactccgca cttccgatac
    21301 gaaccgcgag gctccaaccc gtgccgcccg agtcgttgta tgagtgaggt cctcgatcag
    21361 gccgaaatcg acgccctact ggcatcggtc accatgaccg agtcgggcgg aacgtccgca
    21421 agtgccggca aagcggccgg cctgccgcgt gcggacggcg gtccagcccg cagcccgccg
    21481 tcgaacgcca gcacctacga cttcaagcgt ccggaacgcg tcagcaagga ccacatccgc
    21541 gccttgggct caatccacga gggcttcgcg cgcaatttcg gcgcgacgct ttcgggattc
    21601 ctgcgcacga tcatcgacgt gcgcgtggtc ggcgtcgagc agcttaccta cagcgagttc
    21661 attcactcgc tgcccaaccc gacctgcttc gccatcctgc aggcgccgcc gctcgagggc
    21721 cagatgtgtc tcgagcttag cccgctgatc gtctttccca tcatcgaccg cctcctgggc
    21781 ggcagcagcg ccgagttgta catcccgcag cgcccgctga cctcgatcga atggcggctg
    21841 atcaaccgcc tggtcgaacg ggccctgcaa cacctgtccg aagtctggcg caacctggtg
    21901 gacgcgcact tcgaagtggt ggacgtcgaa accaacccgc aactcgtcca catcgtcgcc
    21961 ccatccgaag tcgtcgtctt catcaccttc gagatcaaaa tgggctcgtg cgccggcacg
    22021 atgagcatgt gcatcccctt caacaccatc gaatccgtgc tcagcaagct gaccacgcag
    22081 tcgtggttcg gctacaagtc caagggcgcc accgacgtgc aacagcaacg cttgctgcac
    22141 aacctgaccc gcagcctggt cgacgtcacc gcctatctgg gccagacacg catccgcctc
    22201 agtgaactcc gggcgctgcg gcccggcgac atgatcgtgc tcgacaaacc caccgatcgc
    22261 gaattgctgc tgcagatcgg cggccgaaac aagtttgccg gcgtatgcgg cacgctgcgc
    22321 ggccggcgcg ccatgcgcct ggcccgccgc gccgaaatcg acgagccttt gtaagtaaga
    22381 gcctctgatt ccggctcaat ccagccgaac tttccgactc gacgctgtgt tggcgcgcgc
    22441 ctcgctcaac ggctttgccg cggccgggca gccgctgctc acggccgcca gtttcggccc
    22501 ggtcgtcatg gtgatgtggg gaggcaacag aatgccggct gaaaccgcca ttttgatggc
    22561 gtcttccacg gaccagtcca tttcgatcac atccgagcgg cgcaagacga agacaaagcc
    22621 ggtggtcggg ttgggcgtgg tcgcaatgaa caccgaacac agttcctcgc ccgtcagcga
    22681 gtctttgaac gtgtttgtga taaagcccac cgtgcgcgtc agctcgttcg gaaacggcac
    22741 cagggccacg cgctgaaagt tctgcttctc atccgcgccg aacagcccca gcacctgctt
    22801 cagcgtgcgg tagatcgtct tgacgaacgg cacccggtcg agaatcagct cgaacagttc
    22861 cagcagccgc cgcccaatcg cgttggccgt gaacatcccg atcacgtaca gcagaaagaa
    22921 cgtcagcagc accgagaaga tcgaaatgcc ccagcgcatc ccgcgcggca gcgcgtcgaa
    22981 gcccgcggcc ggcagcgtgg tcgggttcgt cccggtggcg ctgaccgccg agacgttcac
    23041 cccggttgat tcgagcaaat ggtgccccca cgagctgaac aacaccgcgc tgaccagcca
    23101 gaacgaggcg tcccggaacc agccgaacac caccctcagc agccacagcg tggtcacaat
    23161 cggcaatatc gtgatcaaac cggccgtgac ccgcgttctg accagggctt ttatggtggc
    23221 cgtgatgcgg ctgcggtggt gcttcgattc ttctccgccg tgagtgcttt tcatctgcac
    23281 gaattcctgt gcggcaggca gcgcacggat cgaggtatcg ttggctcgca atgccacgat
    23341 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggccccgc ggccctggtg
    23461 cagggccggg tactgcctgc gactccctgc gcggccggtg cgcgctcgac cggtcctacc
    23521 ctttttgcgc acctctagcg tctcgcaccg ctagccctac gaaagccaga aattcgcgtt
    23581 tcgccccggc cggcgccgga tgcaaccctc ctgcgccccg ccgggtctac agtcgatcct
    23641 catatggcta ttgccgctct taacaggagg aattccgagt gacgaaagct accagttttt
    23701 ttgccaggcg ctccggcgtt atcgtggccg gcctggttat cggcgggttg gcggcaacgc
    23761 tacagaacgc cgacctcttt tccggccgta gcaacccacc caacatgggc gtgtgcgtgg
    23821 cgtgcttcac gcgcgacatc gcgggctccc tgggcctgca ccgtgcctcg gccgtccaat
    23881 acctgcggcc cgaaatcccc gccttttgcc tgggcgcctt tatcgctgcc ctggccttcg
    23941 gcgagtttcg cccgcgaacc ggctcctcgc cgatcgtgcg cttcatactg gggatgttcg
    24001 ccatgatcgg agcgctcgtc ttcctgggct gcccatggcg agcgatgttg cgcctggccg
    24061 gcggtgattg gaacgccatt ctcggactgg ccgggctggt ttcaggcatc gggctcggag
    24121 tgcttatgct acgcggcggc ttcagcctgg gtcgggccta cccggcccgc gccgtggccg
    24181 gcttggccat gcctctgctg atgggcgtgc tgctcctgct cgcgattttc tatcccgttt
    24241 tcggcgaggc cggccaggcg gcgttgttcc gcagcgcgaa ggggccggga gcaatggcag
    24301 cgcccatcgt ggtgtcgatc ctggtcgggc tggtgatcgg atttctggcc cagcgcacac
    24361 gcttttgcac ggtgggcgct tttcgtgact tgcttctgat gcgcgacttt cacctgctca
    24421 gcggaatcat cgccttcctg gtcgccgcct ttgccctgaa cctcgtctat gggcagttca
    24481 agcccggctt caccctcggc ctcgactccg atggcaagcc tattcagcaa ttcgcggcgc
    24541 acagcacgca ctggctcaac ttcgccggca tgctgctggc cggcctttca ttcacgctcg
    24601 ccggcggttg tcccggacgg caggtcttct tgagcggcga gggcgacggc gacgccgcaa
    24661 tcttcgtact cggaatgctg accggcgcgg ccgtttcgca caatttcaac ctggtggggg
    24721 cgtcgaagtt cgccccgtac gtggtggtga ttggtctggt ggtggtcggc gccattgggc
    24781 tggccggtcg cgaagttttc gctcgtaagg aggccaaggt atgtcaacca gtgtagatgc
    24841 ccgcgggctc tcctgtccgc agccgatgtt cctgacgcgc aaggccctgc tcgaagccgg
    24901 cagtggcgag gtggtcgtcc tggtcgacgg tgccgatcag gtcgataact gtacgcgaac
    24961 ggccgagcgc ctgggttggc aggccacctg cgagcaaaag accggccccg aaggcagggt
    25021 attcgagctg actttccgca agtgagcgct gtgaactacc tcgataacgc cgcaacaagc
    25081 tggcccaaac ccgacaccgt ggccgaggcg atgctgcgct acctgcgcga cgtcggcgcc
    25141 agtccaggcc gcagtgctca tcgcctcgcg atccagtccg agcgcctccg tcttgaggcg
    25201 cgcgaggcgg tggccgagct ggtcggcctg cgtgacccgc tgcgggtcgt ctttacgcat
    25261 aacggcacgg ccgccattaa cttggcgctc ttcggcctgc tcgaacccgg cgcgcacgtc
    25321 gtcgccaccg gaatggagca caacgctgtc atgcgcccgc tgcgggccct cgaacgccgc
    25381 ggcgtctcga tcagcctcgc cgcttgccgg cccgacggaa ccatggagcc ggctgccgtg
    25441 gccgagtgcc tccggccgca cacccgcctg gtcgtcatga accacgcctc gaatgtgtgc
    25501 ggcacagtgc tgccggtgcg gcaggtcggc gcgctggccc gcggccgcgg aattcccctc
    25561 ctggtggacg cggcccagac cgccggctgt tggccgatcg acatgcacgc cgacaacatc
    25621 gacctgctgg ccttcaccgg gcacaaggcg atgctcggtc cgaccggcgc cggcggcttg
    25681 gccttgcggg aggatttcga catctcgcgc ctgccggcac tggttcacgg cggcacgggc
    25741 agtcgctcgg aacacgagtt tcaaccggat ttcctgcccg acaaatacga ggccggcacg
    25801 ccaaacgccg ttggtctggc cggcctgaca gcgggcgtgc ggtacgtgct gaaacgcggc
    25861 gtgg
//
LOCUS       DAOSYS010000017        24920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1644_length_24920_cov_5.411422, whole genome shotgun sequence.
ACCESSION   DAOSYS010000017 DAOSYS010000000
VERSION     DAOSYS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24920
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_24920_cov_5.411422"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..898
                     /gene="queC"
                     /locus_tag="PLU99_02030"
                     /pseudo
     CDS             197..898
                     /gene="queC"
                     /locus_tag="PLU99_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
     gene            complement(1273..1343)
                     /locus_tag="PLU99_02035"
     tRNA            complement(1273..1343)
                     /locus_tag="PLU99_02035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1309..1311),aa:Gln,seq:ctg)
     gene            complement(1573..2568)
                     /locus_tag="PLU99_02040"
     CDS             complement(1573..2568)
                     /locus_tag="PLU99_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15864.1"
                     /translation="MPEARGRHETGAAAASAQAAGSIIAGAASSNYRLMSGPWERFRQ
                     NRPAVTALALLLLISAACFGTLGWSAGRMRVQQLDAARQPPSRTHILGTDELGRSLLW
                     RTLYGGAISLSLGLLAAGVSIVIGTAYGLVAGYLGGRWDELMMRAVDVLYTLPYMLLV
                     MLFAVSIGAWLESRRVLGGEWSRFFVLALAIGGVSWLTMARVVRGQVLSLREQPFVEA
                     ARALGLPTSRILLRHLLPNLSGPILVFAALTVPQAILQESFLSFLGVGIQPPQATWGS
                     LAADGVHLLNPIQFDWWLVVVPCVALSVTLLALNFVGDGIRDALDVKQSRGVSPR"
     gene            complement(2561..3496)
                     /locus_tag="PLU99_02045"
     CDS             complement(2561..3496)
                     /locus_tag="PLU99_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO15865.1"
                     /translation="MFAFVLRRLATFPIVLLVMYTAAVLLVMAAPGEGLSTGEKEMPP
                     EVLAARRHAFNYDQPWWQRYFWTWPKRLVWDGDLPAHQYEDWTVVEILRSALPASLQL
                     GVLALSLAVVMGLAVGVISAARRGRWLDHLSVGLALVGISLPTFVTGAVLLIFFAIWL
                     HAVPVGGWGRPAQAILPAFTLALPYAAYIARLTRSALIDVYAEDYIRTARAKGLSEVR
                     VLFDHALQNALLPVLSYLGPAAAAIFTGSFVVEKVFTIPGMGTHVVESINNRDQSLIL
                     ATVMVYGVFLASFNLIVDLLYGVIDPRVRVGARHA"
     gene            3705..3905
                     /locus_tag="PLU99_02050"
     CDS             3705..3905
                     /locus_tag="PLU99_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15866.1"
                     /translation="MAEYLVQINVRRMSEELSRWLGCCVSAADVRELLRKVGFSESPL
                     GWITTDVRPCLLAYLPPGATLF"
     gene            complement(3963..6140)
                     /locus_tag="PLU99_02055"
     CDS             complement(3963..6140)
                     /locus_tag="PLU99_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO15867.1"
                     /translation="MKALRVHVVSMGRAAAILLAWGLAGSAFAEQDKYLIVSASEYVN
                     SAPLNQFIEFRTARGYDVSVYNVSPGTSRDDIKAHIQSLWGTPDAPDFLLIVGDTSGA
                     TSTATTIPHWTGGGSRQAPTDLPYACMDGPGDWYPNMFYGRFSVNSVNMLAQVVEKTI
                     VAESGGTGDPSYTRRAVMLATDDPTALAAALHDSIISTYLEPAGFTATRVYAGVGGGT
                     AHVAAAVNEGVMFTVYFGHSGSSGWSSPGFHTSDVMALTNEGKYGIAFGWSCSSAAFT
                     SDECFGEAWLRAPHKGAAGYLSASSFVWWGTESAWESSRRMERNFFRAFFEKNLWRVG
                     VAWQAALWDILADPDFGPGHDHTRNIFEEFVLLGDPALRLPFRVLDLTAPAAWPEFVP
                     PDVDTTLVLRIQTAAETYDSGLLHYRYNGGEYQTTPLVALGEDLYAGTLPAPPCQALV
                     EYYFSATGHAGGTVYFPEHAPDGEVLSTVAATTSTLFVDNFEIGEGWTVQNDPGLTSG
                     GWQRAVPSAVGGTGAPMVDYDGSGKCFVTDNAPDADVDGGPTRLISPIIDVSGWNEVY
                     VRFGRWMYCDDILPPAQDFLEVEVSADGGATWILAERVWANGGWAKQDIRVPDYVPLT
                     PQFRMRFSINDTPNNSITEVALDGVWIYERGCEPPPLLGDLNCDGAVNVFDIDPFVLA
                     LTEPAAYASVYPDCDAMLADVTGDGAINVFDIDPFVSLLTDSR"
     gene            complement(6315..6773)
                     /locus_tag="PLU99_02060"
     CDS             complement(6315..6773)
                     /locus_tag="PLU99_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO15868.1"
                     /translation="MTISQTAEYALRAIVWLARDPDRPLGTPRIAQATRVPPGYLARV
                     LQALARAGLVESNPGRGGGFRLKRSPADITVLDVINAVDPVERIVRCPLGLKKHRGHL
                     CPLHRRLDEAAALVERAYAETTIAEILAEKSPAEPLCQEAEEEPEFASKK"
     gene            6961..7701
                     /gene="ric"
                     /locus_tag="PLU99_02065"
     CDS             6961..7701
                     /gene="ric"
                     /locus_tag="PLU99_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOO15869.1"
                     /translation="MTSIDLTAPVGQLVADRPARARVFERFDIDYCCGGKTRLDEACA
                     NKGLDPQEVLRALEQAESSAAVPDETDWRHVSLADLISNILNTHHAYLKRGLPELAQL
                     AAKTRQVHGASHPELAEVQEVFTELRAELEGHMMKEERILFPLIQGMESGAACEGFCG
                     SVQNPIRVMEYEHDSAASALQRLRHLTGDYTPPADACNTYRALLSGLAELEADLHQHI
                     HKENNILFPRAIELEATLLHTAPVDRHE"
     gene            7742..9679
                     /locus_tag="PLU99_02070"
     CDS             7742..9679
                     /locus_tag="PLU99_02070"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOO15870.1"
                     /translation="MHPATIPSTALPPSDTTAAAIQPQTMIPDLLRARPHLRDVFDRY
                     GLRGCGGAHGPAETLAYFARAHGVELERLLSELRQADRTVPHPAAAHARGGVAPRPLP
                     VHFADTIYRPFFKTAIAIALSAGAVWGAYLLIRIALAGSFTAISIHDINAHGHAQIFG
                     WVGLFVMGFAYQAFPRMRHTTLWRPGLALFTFYLMLAGIVSRTLGEPLYAVPGMRVLA
                     LAGATSEVIAIALFALVMTRTIHACGHALTWTDGYVFAAMAFFLLQAGYEWGLLLATT
                     GAATRADLLELVATWQAPLRDIQIHGFALLMILGVGLRMFPALFDFPARSPRLVRNCL
                     FLLLAAVAGEVAFFLAMRLTGQSAWAAPLYLSMLVLAGACVALTYRWLPCVPAGRSGE
                     SPTSPPDRSVKFLRASMLWLHLSLLLLLLAPLYMLVLLPQSPWLSSSGAHSAEIHFSH
                     AYYGAIRHAITVGFISLMILAMAAKVVPTLNGVDLRLLRPLWLPFILVNLGCALRVSL
                     QIATDFAAVAYPLVGISGVLEVSGIAIWGVHLWRIMNGWQPAAAPDAGPIHDISASPV
                     PRITADDSPGRVVERFPATLPVLLQHGLTPLANPLLRRTLGRSITIRTAAAMRGISLE
                     AMLAALNQSIQARASLRTRRD"
     gene            9883..11142
                     /locus_tag="PLU99_02075"
     CDS             9883..11142
                     /locus_tag="PLU99_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO15871.1"
                     /translation="MLTVHVVFTILYAALFSNVIWLMPLLVRLQFGSPDPAWRDWQTI
                     AVTAAIPTFMTLSIFWGELLRHVSIRRYLAIFWVVAALPLGCVALVQNYWQLLVLHVI
                     ASAGAAGWMPVNGRLLKHFYPDQARGRAYAVLNVATLGGAAIASYWMGLWMESHPQSF
                     RVYFPIASVAQIVGIAILLWLVRHTNAEQKRESQPARTEPPAAPSLRMLLRPLLHMGA
                     VLRADRAFMMYEKAFMTYGAAYMLCEALLPVLATARLGMRYEDYAHSTLTVSRAVMVI
                     FMLPFGWLLDRIGPVRVSGLAFGVLCVYPVLLLAAGDASGVALASVAWGIGLAGVMMS
                     WMLGPMALAGTPENVPVYVAIHATLVGVRGIVFQIVGMVLYRVTQSFVWPLIAAAAAF
                     AWAAVQMWQLHRSTHWPAPAKAAPEPG"
     gene            complement(11197..12447)
                     /gene="rsgA"
                     /locus_tag="PLU99_02080"
     CDS             complement(11197..12447)
                     /gene="rsgA"
                     /locus_tag="PLU99_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOO15872.1"
                     /translation="MSKKKAGRKIRVRFRQNRLAPKRRGDLTRRYHAESEAVEDTERR
                     ESVRARGELSRMRTIIVGEGDAPLVDEKLWLRGDVVAVHGLVCFVDDGQGRMWACTVR
                     RILRTLLIESRSPVTVGDRVWFSDQTRMARGELVGVIERVEPRRSVLSRRDARGRQHT
                     IVANADQLLIVSSVAEPKLKPHLIDRYLVAAHKGNLRPVVCLNKIDLAEQGVDPEDVL
                     AYTQTIAADCDDPLDEPEHEAITVGSVLDELRGIGYRCLTTSAATGAGLDELRSVLTG
                     HITVLSGQSGVGKSSLLNAIQPGLNLAVAKVSEETEKGKHTTTHARLLRLDFGGYVVD
                     TPGIRAFDLWEVNPAELEVFFPEFVEPLQHCHFKDCLHRDEEGCAVRAAVEAGQISLR
                     RYLSYLKMFEETAAGTGWQRDKAK"
     gene            12672..14819
                     /locus_tag="PLU99_02085"
     CDS             12672..14819
                     /locus_tag="PLU99_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOO15873.1"
                     /translation="MQYRHAAPWLLLGLGLLLGPRLLIAAEPAKPTDEIRDKIKAAGD
                     AEKWNADLVYVFDYTDVTVKPSGLGVAKNHKLLKILRDGGIRSQAVQRFTFDPHTNRL
                     DLISVRIHRADGTVEEIPLDNPIEQPQPASGIFWPTAQYLFTLPHLAIGDAVETQTEM
                     TGFNVAYLGRDQGIEGSRDQEGAAAVASAAKPKSEPKAPARAANAAAQPSAEEPGFNN
                     LNALGVPLQPPVPGHWHDEVHFWSGYPIIEKRYTVRVPKDKPLQAEVYNGELRSAVLF
                     EGDQLVYTFEKTNIEPFKSEPSMESTPNVGTKLLCATLATWEEKARWLYEVSEPQFAW
                     DDAIAAKVKEVIKDCKTDEEKYTALNHWVAENIRYAGTSRGMCEGYTVHRSIETFHDR
                     CGVCKDKAGMLVTMLRVAGFDSYLTMTMARQRVDRVPADQFNHAVTCFRDKDGNLTLL
                     DPTWMVKSRDNWSTLEPLQHVVYGIPEGKELSQSPYFPPEDCTVSWKGQTAISEDNEL
                     TGGLNFYSTGAPEGRLRRALAAYPADERHEVFEASFARISPVTKIGSLTCMDPVDFSE
                     PLKLQATFGAEDFALGGGNKRYFKLPLLQTPLGDRTLMDLPGTYGLETRKYGVRLWAT
                     RLVKVDETVKLPPGWKVVESPKPVNIDGPSASLQFDIESTPGQVHYTCELRIKKWQIP
                     PEEYANFKEVMEKFDELAGPVMVCELEGAHVER"
     gene            14806..16728
                     /locus_tag="PLU99_02090"
     CDS             14806..16728
                     /locus_tag="PLU99_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOO15874.1"
                     /translation="MLSVKEGIKGSRDRGIKSAFAVAVFLLAALALPPVAWADTAPQP
                     DGVIELWEQHWTINDDGSTVYREKQHVRLNSDRTHREFADPRLTYNAETDNLEIITAR
                     VKRADGTYRELPDYSHVLVSPDITAGWPAFAAIRQHVLVMSGIETGCVVELDYQITSK
                     PGARPALAADVRLDHAYPVLERVLSITSPAAANIRPSVIGPGGAQIGTKPEVSSSTSV
                     KSGLPYETKTWTFRNLPAHPAEPRCAPWQVRCPRFAFNSGGDWHRWMQRTTSDIESAA
                     NECEITAKLAADWTKDVLQPADKMRAIQEKLAAVFNVVEFDSDWQRGGLRPASETIRG
                     GYGLPQEATAVLLSLARAAGLPARAAVLVNDDRWLESAPHDSQVAAYVVLLDGGQQPE
                     IWDARHGRIVRDSRWAGTSLLIMGDASAMRTPLPAWTQADESRCVVGGTIKMKADGTY
                     AGTVSLATSGLFVAPESLRSSEAQKSRVSALLGRVLPEVTVESFAVKELSDNEFAVEA
                     KVKSSKALKKVGGAYWLQMPADAPYAANVTLPLAYSERNEPVRITGPFVEQVKLTVEY
                     PENWTVEAQPVEVKAVKGEWGEAEQRITPGEQRVTISRNTRIAQRDLAPKAFLNAREP
                     LNTLRTEAARTLVLKP"
     gene            complement(16774..17808)
                     /gene="miaA"
                     /locus_tag="PLU99_02095"
     CDS             complement(16774..17808)
                     /gene="miaA"
                     /locus_tag="PLU99_02095"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOO15875.1"
                     /translation="MPAPLRGLGMARAAWYGTGGLNMAPIGRIGLEKRVITILGCTAV
                     GKGTLARAIAGRLGAEIVSIDSMKVYRGMDIGTAKPSAEARAAIPHHLIDVAEPWEPF
                     SVARFIELADRAVGEVHGRGRPVIAVGGTLLYFKCFYQGLFAGPSADPAIRAEIRRRA
                     EVEGIEALHAELARIDPEAAGRIHRNDLRRIERALEVHRLTGTPISVLQREWDSQSIR
                     RPEWDWVLIGLRRPREVNNRRINERVRRMVAAGLVEEARRLWADPRGLSDQARQAVGY
                     AELFEHFEGRMSLDEAIERIKINTRHLAKQQRTWLRRLERVNWIDVEEADDLERIVGL
                     VLPLACVTGS"
     gene            18034..18327
                     /locus_tag="PLU99_02100"
     CDS             18034..18327
                     /locus_tag="PLU99_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15876.1"
                     /translation="MRTSFLLAASAALILTTLGCVSVKAPERIDIGGSRPEPVDTGRV
                     PPIATVEQGRQELYRAYQNIQYLEQQNCRLKEKADKYKRERDECRKRLERYED"
     gene            18539..>21073
                     /locus_tag="PLU99_02105"
     CDS             18539..>21073
                     /locus_tag="PLU99_02105"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOO15877.1"
                     /translation="MAVTFVLGRAGSGKTRYCVDRLLSELQRSGDTRRLVLLVPEQAS
                     FQMERTLATRAPNGGYSRAAVLSFSRLTHQALAELGDTPEVVTPAARVMALRAVTTRM
                     GEGLRYFRHVAQTAGFFTQLERLVEELLSEAVSPESLAEAVGALDPPAARRKIAELSA
                     IYQGYLEWLGRERIDPAARLSLLRSRLAGLTWLRDSLFWVDGFAGFTGQELATLVELA
                     RAARGLTLTLLLDPDSPVIRANAAPGMLDLFRRTELTYQKLRTLFESAGVPCTDPIIL
                     HAAPLPRFAQAPQLARLEAGLAAPFAAPASPASASATSPDQTVSLIECVTHREELRQA
                     ARSIRQQIIESGGALRFRDFAVIARDLEPLVPIIADVFTEYEIPYFLDRRRPMRSHPL
                     SRLVEALFQAIAEDFSVTATTRLLRTGLLPIEREQAEALENLIVGQAIHGLAAWRQPH
                     WTFDDLSNLAINPQRAAPNASRVPLLESAASSSARTPNQPDSASSPSSTEPAPTELDS
                     ARLAIMAAIEPLLNLAAQDPPPTANLWVRTLIDLLEQMSVRRRLESWIAAHRQERRWE
                     QAETHRVAWETLCGALDDLHTVLGDERLRLDEVARLLSATLREQTLGLAPPTLDQVLV
                     SSIERSRHPEIKFAWVCAFNEGIFPARPAEDELLDTGERDALTKAGLPAPASHREDSF
                     GERLLAYIAFTRPSVGLTISYATVAEDGQPLLPSPLLADVRRVLPELQIARPQPHPPP
                     VSLAELARGYLHGFEGSRDLGIKGSRSTAAGGSTTRYGKLVAELLAAPGTGPRLKHLL
                     RGLDYRNAAESVDATYRTYPAGIAWRGSPSEAETYLDCSFKHFAQYA"
     assembly_gap    21074..21173
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21174..22019
                     /locus_tag="PLU99_02110"
     CDS             <21174..22019
                     /locus_tag="PLU99_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO15878.1"
                     /translation="LCDFVGELMRAHAGRWRRGQFEPHLLERRFGSETDPDGLPGLDL
                     TLTDGRRVRLRGVIDRLDICRQDGRTLVLVYDYKSSISPFSGAAYLTGGALQVLTYLL
                     AIRTALPTRDPLTLAGVFLAPLFPDEAVLKIQYAQAAEADQEAMYLYRPRGVFIESAA
                     RLLDPQLGQTSSPVACMRLKKDLTFDRTQSRDVVDENGLESRLALARQTLLMAAEGIV
                     AGKVEASPLVERHELACGHCEFHGLCRFDPLINRARAAERVLPTLDQFAAQVETGDTN
                     EGGAE"
     gene            22016..>24920
                     /locus_tag="PLU99_02115"
     CDS             22016..>24920
                     /locus_tag="PLU99_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOO15879.1"
                     /translation="MNLTPSQQQAIDHRGCNLLLSASAGSGKTEVLARRVLALIADPQ
                     HPCPVERLLVVTFTRAAAAELRVRIGRMLREAAAKTSDAHMRDHLRRQEILVDSADIG
                     TIDAWCARIVRAHAAEIGVDPAFVMLSEEDAWLLRRHVLDELFTWIYFAADPLAQAAR
                     EWLKRTTRPDDKFLRRHVEKLNQFRENLVEVDAWLARQRDLHAAGPDELRARACVTLT
                     AALAQECAFQHQQLTALLAGAARELSTVLQPYHESLADWNARLTQEPQEAALLAVLDE
                     IDAFKLRKPRGLPPDAAALCTDIQTRWLKRRLQACWSRDEAVRLLDTAPAAAALVTTL
                     LDLEQRFHTRLSEIKRSQATYEFGDVLRMALDLLAPPAATGPRTPSAIAQRLQQRYEH
                     VLVDEYQDTSPVQVEILRLVTRPQPGRSNRFMVGDIKQSIYGFRQAEPRLFSELLQDF
                     AAGRQEGIVQYLSDNFRSHPAIVAALNRLFAMLFDPALGGTAFAPDERLRAGRDEIAN
                     PSLDANPRVELHIIEQPAGRSAAQNEAEDSEDNNAVPLERMEREALLAADRIHALLRD
                     GVQVPERGPDGRLSLRPLRLADIVILLRSAKQNAAVVARILRQAGIACITSGRDALLD
                     VPEVMDVRNVLALLANRRQELPLAAYLRSPMVGLSLPELLQIRAAAPHADFCDAVATF
                     CTGKPRDQAGAALQAKLRAAMAQLDRWAITAREQELPELLRQIYNDTAWLLFAQALPG
                     GEHRVALLRALERFAIDFARRGQHGVAEFDAYLEDLATAELDPGVPVAAGENVVRIMT
                     IHAAKGLEFPVVFLLNTGSRFNLSRTNEPLQCDASTGLGLTFFDYPARLKVTSARHPI
                     AARHGRTREIEEELRLLYVATTRAREQLIICGHAKPDAWDTACTQFATAGGPPPLPTR
                     LGATSLLDWVIMAAAAAGLHERGTNTCAALEVTLHQPQEENGRGSLPSSAPPEP"
BASE COUNT         4508 a         8569 c         7509 g         4234 t
ORIGIN      
        1 cgtgattcgc tcgctcgtaa ccggaatgcc gtacccgtac gtggtggcgc gcagcgacgc
       61 tcgtttgtgc ggcgtagtga tggacatcgg accggagggg cgcgcgctca gcatcgatcg
      121 cgtgagtttg aaagacgaag gctggccgcc agccgagggc gaggaatagg agcagaaact
      181 cgcaacacgg gcggttcgga tgtctgcaca gcgtgaaaac cggcgtgcgg ttgttctgct
      241 ctcaggcggc ttggacagcg ccacggcctt ggcgatggct cgcgcggctg gttttgagct
      301 gcacgccctg acgtttcgct atgggcagcg gcacgtcagc gaactcgagg cggcgcggcg
      361 tgtggcggct gcgatagggg tagccgagca ccaggtcgtc gaaatagatc tgcgcgtgtt
      421 cggcggctcg gccctgacga gcgatctggc ggtgccgaag gatcgcgacc tggaggcgcc
      481 gggcattccg atcacgtacg tgccggcccg caacacaatt tttttggtgt atgggctcgc
      541 gttcgcggag gtgcgcgagg cgcgggacat tttcatcggc gtcaacgcga tcgattacag
      601 cggctacccg gactgtcgcc cagagtacgt cgcggcgttc gaaacgctgg ccaacctggc
      661 aactcgcgcc ggagtcgagg ggaacagaat caggattcac gccccgctgc tatatctgag
      721 caaggcggag attatccgcc gcggcttggc gttgggcgtg gattatgggt tgactcgcag
      781 ttgctacgat cccgacgagc aaggccgggc gtgcgggcac tgcgatagtt gccggctgcg
      841 cttggaagcg tttgcgaagg ttggcgtgcc cgacccggtt ccgtacgcga gaacgtagaa
      901 gcccaagtcc gggaagctaa gttcgcgcaa aaaaatgggc cggcggtcgg gacgtgcggg
      961 ggggacacgt gcgccgacgc agtccggccc ggggctcggg gctaagggca aataataacg
     1021 cgatagcaca accgtggcaa gccccaagcg ctgtccttgc cggcgcggct cgggctaccg
     1081 cccctcgatt ccgactagcg gtatccggcg atctcgcatt gtggtgcggc acccggggtg
     1141 ccggtaaacg aagctagact ccgcgtcacc ggagatgcgc gaacgggagc accgggatag
     1201 caccacgggg tggtaaaaaa aaggccggac accacggttc ataagtcccg tgctttccgg
     1261 cctttaggtc acctggggat ccaggattcg aacctggact aactgatcca gagtcagtcg
     1321 tgctaccgtt acaccaatcc ccaaatctcg tgccaagtct aggggagaat cgttcgcaaa
     1381 tcaatcccct tacgttagca aatcaatcac ccaccgttgg caaatcaatc ccccgccgtt
     1441 ggtaaatcaa tcccgtcgtt gaggaagagc agagccgggg cgtgtcgccc cgaaatggcg
     1501 gcaaaggcgt cgcggcgcgc gatgcgggcg ggatgggcgt tcatcttcaa cacgatgcgg
     1561 acgagccggc gttcaccgcg gcgacacgcc ccggctctgc ttcacgtcga gggcgtcgcg
     1621 gatgccgtcg ccgacgaaat taagcgcgag cagcgtcacg gacagcgcta cgcacggcac
     1681 gacgaccagc caccaatcga actgtatcgg gttgagcaga tgcacgccgt ccgcggccaa
     1741 cgagccccac gtcgcctgcg gcggctgaat cccaacgccc aaaaagctca ggaacgattc
     1801 ctgaaggatc gcctgtggca ccgtcaaagc tgcgaaaacg aggatcggac ccgacagatt
     1861 cggcagcagg tgccgcagga gtatccgaga cgtcggaaga ccgagcgctc gggccgcctc
     1921 aacgaacggc tgctcacgca gcgagagcac ctgcccccgc actacccgcg ccatcgtcag
     1981 ccaactcaca ccgccaatcg ccagggccag cacgaagaag cggctccact cgccacccag
     2041 cacgcgccgg ctctccagcc acgcgccgat cgaaaccgca aacagcatca cgagcagcat
     2101 gtacggcagg gtgtagagca cgtccaccgc ccgcatcatc agctcgtccc agcgtccgcc
     2161 caggtagccc gccacgaggc cgtacgcggt tccgatgaca atcgaaaccc cggccgccag
     2221 cagacccagg ctcaacgaga tcgcgccgcc gtacaacgtg cgccacaaca agctgcggcc
     2281 caattcgtcc gttccgagga tgtgcgtgcg cgacggcggc tgcctggccg catctagctg
     2341 ctgcacccgc atccggccgg ccgaccagcc gagtgtgccg aagcaagcgg cgcttatcaa
     2401 cagcagcagc gccagcgcgg tgaccgccgg gcggttctgc cgaaagcgct cccacggccc
     2461 gctcatgagg cggtaatttg agcttgccgc gcccgcaatg atcgagccgg cggcctgtgc
     2521 gctggcggcc gccgcgccag tttcgtgtcg cccgcgcgct tcaggcatgg cgcgccccca
     2581 ctctgacccg cgggtcgatc acgccgtaca gcaggtcgac tatcaagttg aaactcgcca
     2641 ggaagacgcc gtaaaccata accgtcgcca gaatcaggct ctggtcgcgg ttattgatcg
     2701 actccacaac gtgcgtcccc atgcccggaa tcgtgaagac cttctccacc acgaatgagc
     2761 ccgtgaagat ggccgcggcc gccggaccga gatagctcaa caccggcagc agcgcatttt
     2821 gcagagcgtg gtcgaaaagc acccgcactt cggacaggcc cttcgcgcgg gccgtgcgga
     2881 tgtagtcctc cgcgtaaacg tcgatcaagg ccgagcgcgt caggcgcgcg atgtaggccg
     2941 cgtaaggcaa cgccagcgtg aacgcgggca gaatggcctg cgcaggccgg ccccaccccc
     3001 ctaccggcac cgcatgcagc cagatcgcga aaaagatcag cagtacggcc cccgtaacga
     3061 aagttggcag agatattccg accagggcca agccgaccga gaggtggtca agccatcggc
     3121 cgcggcgcgc ggcggagatt accccgaccg ctaggcccat tacgacggcc aaactcaacg
     3181 ccagcacgcc gagttgcagc gaggccggca aggccgaacg gaggatttcg accaccgtcc
     3241 agtcctcata ctggtgagcc ggcaggtccc cgtcccagac caggcgcttc ggccaagtcc
     3301 agaagtagcg ttgccaccag ggctggtcgt aattgaaggc atggcgtcgt gcggccaaca
     3361 cctccggagg catttccttt tcgccggtcg acagcccctc gcccggcgcg gccatgacca
     3421 gaagcacggc ggccgtgtac atcacgagca acacgatcgg gaaggtcgcc aatcggcgca
     3481 ggacgaacgc gaacatcgcg cgaattatgc ggtgcggtgg cgaccctggc cagccctacc
     3541 agcgttcacg gtacctgtcg ttccatggcg cgccggagag gtgcgcagcg ccccccaggg
     3601 cggcacgtcg aagagtcgcc gcggcaaggc ctctcgaaca tggaggccgc taatcacgta
     3661 aacatattgg caggatgaag ctgctcgaca ttgagaggtg ccccatggcc gaatacctcg
     3721 ttcaaatcaa tgtccgccgc atgagcgaag agctgtcccg ctggctcggt tgctgcgtga
     3781 gcgcggcaga cgttcgcgag ctgctgcgca aggtggggtt ctccgagtcg ccgctcggct
     3841 ggatcacgac cgacgttcgg ccatgcctat tagcgtactt gccaccggga gcaacgctgt
     3901 tctgacgctc gaggcatcga ggcgacggcg cggagcagcc cgccaagaag tacctggaaa
     3961 gatcaccgac tgtcagtaag taatgacacg aacgggtcga tgtcgaacac gttaatggcg
     4021 ccgtcgcccg tgacgtcggc cagcatcgca tcacaatccg ggtacaccga ggcatacgcc
     4081 gccggctcag taagcgctaa gacgaacggg tctatatcga acacgttcac ggcgccgtcg
     4141 cagttcaggt cgcccagcaa gggcggcggc tcacaaccgc gttcgtaaat ccacactcca
     4201 tccagcgcca cctcagttat cgagttgttt ggagtgtcgt tgattgagaa cctcattctg
     4261 aactgcggcg tcagtggcac gtagtcgggc actcggatgt cctgcttggc ccagccccca
     4321 ttggcccaaa cgcgctccgc caggatccag gtggcgccgc cgtccgcgga aacctcgacc
     4381 tccagaaagt cctgcgccgg cgggagaatg tcgtcacagt acatccatcg cccaaagcgc
     4441 acgtaaacct cgttccaccc gctcacgtca atgattgggg agattaggcg cgtcggtccg
     4501 ccgtccacgt cggcatcagg cgcgttgtcg gtcacgaagc acttgcccga accgtcatag
     4561 tcaaccatcg gcgcaccagt cccgccgacc gcggacggaa cagcccgctg ccaaccgccg
     4621 ctcgtgaggc ccggatcatt ctggacggtc cagccttcgc cgatttcaaa attgtcgacg
     4681 aatagcgtgc tagtcgtcgc cgcgacggtg ctgagaactt ctccgtccgg cgcgtgctcc
     4741 gggaaataaa ctgtcccgcc cgcatggccc gtcgcgctga aatagtactc caccagcgcc
     4801 tggcacggcg gcgccggcaa cgtacccgca tacaagtcct cgcccaaggc caccagcggc
     4861 gtcgtctgat actcaccccc gttatagcga tagtgcagca agccagagtc gtatgtttca
     4921 gccgcagtct gaatccgcag gaccagcgtc gtgtcgacat ccggcgggac gaactcgggc
     4981 cacgccgccg gcgctgtcaa gtccaacacc cggaacggca ggcgcaatgc cgggtcgccc
     5041 agcagcacga actcctcaaa gatgttgcgc gtgtgatcgt gccccggccc gaaatccgga
     5101 tcggcgagaa tgtcccacaa ggcagcctgc caggccaccc cgactcgcca caggttcttt
     5161 tcaaagaacg cccggaagaa attcctttcc atacgccgcg aggattccca cgcgctttcg
     5221 gtgccccacc atacgaagct cgaggccgac aagtacccgg ccgcgccctt gtgcggcgct
     5281 cgcagccacg cctcgccgaa gcactcgtcc gatgtaaaag ccgccgaact gcacgaccac
     5341 ccgaaagcta tcccgtactt cccctcgttc gtcaaagcca ttacgtccga cgtatggaat
     5401 cccggcgacg accatccgct cgagccgctg tgcccgaaat acacggtgaa cattacaccc
     5461 tcgttcaccg ccgccgccac atgcgccgtg cccccaccca ccccggcgta cacgcgcgtg
     5521 gcggtaaagc cggccggctc caggtaagtg ctgatgatcg agtcgtgcag cgccgccgcc
     5581 aaagccgttg gatcatcggt tgccagcatt acggcgcggc gtgtgtaact cggatcgccg
     5641 gtaccgccgg attcggccac gatcgtcttc tcgacgacct gcgccaacat gttcacgctg
     5701 ttcaccgaga accgcccata gaacatgttt ggataccaat cgcccgggcc gtccatgcac
     5761 gcgtatggca ggtcagtcgg ggcctgccgg ctgccgccgc ccgtccagtg cggaatggtg
     5821 gtggcagtcg acgtcgcccc gctggtatcg ccgacgatca gcaggaaatc cggcgcatcc
     5881 ggcgtgcccc acaggctttg aatgtgggcc ttgatgtcgt cccggctggt gcctggcgac
     5941 acgttgtaaa cggagacgtc atagcccctg gcagtgcgga attcgatgaa ctgattcaag
     6001 ggcgcactgt tcacgtattc ggacgccgac acgatcagat acttgtcctg ttccgcaaac
     6061 gcgctgccgg ccagccccca cgccagcaga attgccgccg cccgtcccat cgatacaaca
     6121 tggactcgca aagccttcat cccagctctc ctatcccccg cgtcgaaaga cgcaatcgtg
     6181 gtgccttcag tcccaatcca ggcgggccga cgcggccata cggggcagcg caacctcccg
     6241 ccgtcgcaat tcagaatacc gcgcgctgcc ccccgaaagc aatctgaact tcaccggccc
     6301 ggccggcccc cttctcactt cttacttgcg aactcaggct cctcctccgc ctcctggcac
     6361 aacggctcgg ccggcgattt ctccgccaga atctccgcaa tcgtggtttc cgcatacgcc
     6421 cgttctacca gcgcggcggc ctcgtccagc cggcgatgca gtgggcacaa atggccgcgg
     6481 tgttttttca gacccagcgg acagcgcacg atgcgctcca ccgggtcaac cgcgtttatc
     6541 acgtccaaga cggtgatatc ggccggagag cgcttcagcc gaaatccgcc cccccgcccc
     6601 gggttggact cgaccaagcc ggcccgggcc agcgcctgga gcacgcgtgc caggtaaccc
     6661 ggcgggacgc gcgtcgcctg cgcgatgcgc ggcgtgccca gcggacggtc ggggtcacga
     6721 gccagccaga caatggcgcg caacgcgtat tcggccgttt gtgaaatcgt catgtccgag
     6781 ctccaggcgc cgggatttgg tccggcgctg tcagggtcag gccgagtgtg ccgacgcaga
     6841 atgtcgtcgt ccgccttgac ctctggatta tgccgcctaa actcataaag tcaacctgaa
     6901 cgtttaggtt taggtttatc gcaaccgagg ttgagcagga acctgtaagg agcttcacaa
     6961 atgacgtcca ttgatctgac cgcccccgtc ggccagctcg tcgccgatcg cccagcgcgc
     7021 gcccgcgtct tcgagcggtt cgacatcgat tactgctgcg gtggcaagac gcggctggat
     7081 gaggcctgcg cgaacaaagg actcgatccg caggaggttc tccgtgcgct ggagcaagcg
     7141 gaaagctctg ccgccgtgcc ggacgaaaca gactggaggc acgtttcgct cgcggatctc
     7201 atcagcaaca tcctcaatac ccatcacgct tatctcaagc gcggcttgcc ggaactggcc
     7261 cagctcgccg ccaaaacgcg ccaggtgcat ggcgccagcc atcccgagct ggccgaagtc
     7321 caggaggtct tcacggaact gcgggcggag ctcgaaggcc acatgatgaa ggaagagcgc
     7381 atcctgttcc ccctcattca gggcatggaa tccggcgccg cctgcgaggg cttctgcggc
     7441 tccgtgcaga atccaatccg cgtcatggag tacgaacacg attccgccgc ttctgctctg
     7501 cagcgtttgc gccacctgac cggcgactac accccacccg ctgacgcatg caacacgtat
     7561 cgcgctttgc tgagtgggct ggcggaactc gaagccgatc tgcatcagca tattcacaag
     7621 gagaacaaca tcctgttccc gcgcgccatc gagttggagg ccacactcct ccacacggca
     7681 ccggttgacc ggcacgagta gcagcaaacc ccgcggaatt acgctcgaca taggtctgaa
     7741 aatgcacccc gccacaattc caagcacggc actgccaccg tccgacacca cagcggccgc
     7801 gattcagccg cagaccatga ttcccgacct gctccgggcg cggccacacc tgcgcgacgt
     7861 cttcgatcgc tacggtctgc gcggctgcgg cggagcgcac ggcccggccg aaacgctggc
     7921 ctacttcgcg cgcgcccacg gcgttgagct cgaacgcctg ctcagtgagc tacgccaggc
     7981 cgaccggacg gtcccgcatc cggccgcggc acacgcccgc ggcggcgttg ccccccggcc
     8041 tctgcccgta cattttgcgg acaccattta ccggcccttt ttcaagaccg ccattgctat
     8101 cgcgctgtcg gccggcgcgg tatggggcgc gtatctgctg atacgcattg ccctggccgg
     8161 ctctttcacc gccatttcta ttcacgacat caacgcccac ggccacgcgc aaatcttcgg
     8221 ctgggtcggc ttgttcgtga tgggctttgc ctaccaggcc ttcccgcgca tgcgccacac
     8281 tacgctgtgg cggccgggtc tggcattatt cactttttac ctgatgctcg ccggcattgt
     8341 gtcacgcaca ttgggcgagc ctctctatgc cgttcccggc atgcgcgtgt tggcgctcgc
     8401 cggagcgacg agcgaggtga tcgcgattgc cctattcgct ctggtgatga cccgcacaat
     8461 tcacgcttgc ggccacgccc tgacttggac tgacggatac gtgttcgcag ccatggcgtt
     8521 cttcctgctc caggccggct acgagtgggg cctgctgctg gccacaaccg gggcagccac
     8581 acgcgccgac ctgcttgaat tagtggctac ctggcaagcg cccctgcgcg atatccagat
     8641 acatggcttc gccctgctga tgatcctcgg cgtcggcctg cggatgttcc cggccttatt
     8701 cgacttcccg gcccgcagcc cgcgcctcgt gcgtaactgc ctcttcctgc tactggccgc
     8761 agttgcggga gaagtcgcgt ttttcctggc catgcggctt actggtcaat ccgcctgggc
     8821 cgcgccgctg tacctttcga tgctcgttct tgccggggct tgcgtggcgc tcacttaccg
     8881 ctggctgccg tgcgttcctg ccggccggag cggcgaaagc ccaacctcgc cgcccgaccg
     8941 cagcgtcaaa ttcctccgcg cgtcaatgct ctggctgcat ctctcactgc tcctgctgct
     9001 actggcgccg ctttacatgc tggtcctttt gccgcaatcg ccctggcttt catcctccgg
     9061 cgcccactcc gccgagatcc acttttcgca cgcctattac ggcgcgatcc gccacgccat
     9121 aaccgtcggc ttcatcagcc tcatgatcct ggcaatggcc gccaaagtcg tccccacgct
     9181 gaacggggtc gatctgcggc tactacggcc gctatggctg ccgtttatcc tggtgaactt
     9241 aggctgcgcc ctgcgcgtaa gcctgcaaat cgccaccgat ttcgccgccg tcgcttatcc
     9301 cttggtcggc atcagcggcg tacttgaagt cagcggcatc gcaatttggg gcgtgcacct
     9361 ttggaggata atgaacggct ggcagccggc cgcagccccc gatgcaggcc cgatacacga
     9421 cattagcgcc tcccccgtgc cgcgcatcac ggccgacgac tcccccggcc gcgttgtgga
     9481 gcgcttcccc gccacactac ccgtcttact gcagcacggc ctgacgcccc tggccaaccc
     9541 gcttctgcgc cgcacgctcg gacgctcgat caccatccgt accgcggcgg ccatgcgtgg
     9601 catttcgctt gaagccatgt tggcggcttt aaaccagagc atacaagcac gagcctcact
     9661 ccggacccgc cgagactgag acagccgcgt ccggtggcac accgcccttc cagcaattcc
     9721 gtgcccgtcc gcgccgaggt acaatgctat ctttatcgcc agcgcggcgg tggatcgccg
     9781 ccccggccga ctggagtggc tctcaaaccc gtgccgcagc tcacagaacc cgcacctgac
     9841 gcatcgacac cgaatggacc gggcgcgccg cgattgacgg ttgtgctgac cgtccacgtc
     9901 gtattcacaa tactctacgc cgcgctgttc tctaacgtaa tttggctcat gccgctgctg
     9961 gtgcgactgc agttcggctc gcctgatccc gcctggcgcg actggcaaac gatcgccgtc
    10021 acggcggcca ttccgacttt catgactttg tccatcttct ggggcgaact gctgcggcat
    10081 gtctcgatcc gccgatactt agcgattttc tgggtcgtcg cagcgctgcc gctgggatgc
    10141 gtcgcgctcg tgcaaaacta ctggcagctt ctcgtgctgc atgtgatcgc gtccgccggc
    10201 gcggccggct ggatgcccgt caacggccgc ctgctcaagc acttttatcc cgaccaggcc
    10261 cgcggccggg cctacgccgt cctcaatgtc gccaccctgg gcggtgcggc catcgcttct
    10321 tattggatgg gcttgtggat ggagtcgcat ccacagtcgt ttcgagtcta ctttccgatc
    10381 gcctcggtcg cccagattgt ggggatcgcc atcctgctct ggctggtgcg gcataccaac
    10441 gccgagcaga agcgtgagtc gcagccggcc cgaacggagc cgccggcggc gccgtcgctg
    10501 cggatgctgc tgcgcccgtt gctgcacatg ggcgccgtct tgcgggctga ccgcgcattc
    10561 atgatgtacg aaaaagcgtt catgacttac ggcgcggcgt acatgctgtg cgaagccctg
    10621 ttaccggtgc tggccaccgc ccggctgggc atgcgttacg aagactacgc tcattcaacc
    10681 ctgaccgtca gccgggcagt catggtgatc ttcatgctgc cgttcggctg gctgctggat
    10741 cgcatcggcc cggtgcgcgt ctcggggctg gccttcggcg tcctatgcgt ctatccggtg
    10801 ctgctgctgg cggccggtga cgcgagcggc gtggccctgg cgagcgtagc ctggggcatc
    10861 gggttggcgg gcgtcatgat gagctggatg ctcggcccga tggcgttagc cggcacgccg
    10921 gagaatgtgc cggtttacgt cgccattcac gccacgctgg tcggcgtccg cggcattgtc
    10981 ttccagattg tcgggatggt gctgtaccgg gtgacgcaaa gcttcgtctg gccgttaatt
    11041 gcggccgctg cggcgttcgc ctgggcggcg gtccaaatgt ggcagttgca ccggtcaacg
    11101 cactggccgg caccagccaa ggccgcgcca gagccggggt gaaaaggcat gccgagacac
    11161 gaagtgcggc catttgagcc gctaactcat taaaggttac ttggctttgt ccctttgcca
    11221 gcccgtcccg gccgcagtct cttcgaacat cttgagatag ctcaggtacc gtcgcaggct
    11281 gatctgcccc gcttcgaccg cggcccgcac tgcacaacct tcctcgtcgc ggtgcaggca
    11341 atccttgaaa tggcagtgtt gcagcggctc gacgaactcc gggaagaaca cctcgagttc
    11401 ggcgggattc acttcccaca agtcaaaggc ccgaatgccc ggcgtgtcta ccacgtaccc
    11461 gccgaaatcc aggcgcaaca gccgagcgtg cgtcgtggtg tgtttgccct tctccgtttc
    11521 ctcgctcact ttcgccacgg ccaggtttag ccccggctga atggcgttca gcagcgaact
    11581 cttgccgacg ccactttgcc ccgaaagcac cgtgatgtgc ccggtgagca cactccgaag
    11641 ctcgtccaag ccggcgcccg ttgcggcgct cgtcgtgagg cacctgtagc cgatgccgcg
    11701 cagctcatcc aagacgctgc cgacggtgat cgcctcgtgc tcgggctcat ccaacggatc
    11761 gtcgcaatcc gcggcgatcg tctgggtgta ggccagcacg tcctccggat cgacgccttg
    11821 ctcggccagg tcgatcttgt tgaggcaaac taccggccga aggttgccct tgtgcgcggc
    11881 gaccaggtag cggtcgatga ggtgcggctt gagcttcggc tccgcgacgc tgctcacgat
    11941 cagcaactga tcggcgttgg cgacgatcgt gtgctgccgg ccgcgtgcat cgcggcgtga
    12001 gagcacgcta cggcgtggct cgacccgctc gataactccc accagttcgc cgcgtgccat
    12061 gcgtgtctgg tccgagaacc aaacgcgatc tccgaccgtc acgggcgagc ggctttcgat
    12121 cagcagcgtg cgcagtatgc gccgtacggt gcaggcccac atgcgcccct ggccgtcatc
    12181 gacgaagcag accaggccgt ggaccgcgac cacgtcgccg cgcagccaga gcttctcatc
    12241 gacgagcggg gcatcgccct cgccgacgat gatggtgcgc atacgggaca gttcgccccg
    12301 ggcgcggacc gattcgcggc gttcggtgtc ctcaaccgcc tcgctctccg cgtgatagcg
    12361 gcgtgtcaga tcgccgcgcc gcttgggcgc tagccgattc tgccgaaatc gcacgcggat
    12421 tttgcggccg gcttttttct tcgacattgc cctattctat atcgaatcgg acaccgcgca
    12481 acgcgctcgg agctgcttgt ttgaccgcgc aggccgcgaa accgcctcgt ctccgaacca
    12541 gcgccggcac tgcttgaagg ctttctcgct tggtgatttt gcactccccc ggttgaatcg
    12601 gataagatgc cccgactaac cagccggacg gccggcaggg gcaacaggcg attctcatgc
    12661 aggagaggtt tatgcagtat cggcacgcgg caccctggct attgctgggg ttgggcttgc
    12721 tgctcggccc gcgattactc atcgcggcgg agcccgccaa gcccacagac gagatccggg
    12781 acaaaattaa ggccgccggc gacgcggaga agtggaacgc tgacctcgta tacgtcttcg
    12841 actataccga cgtcacggtc aagcccagcg gtctgggcgt cgcgaagaac cataagctct
    12901 taaagattct ccgtgacggc ggcatacgca gccaggccgt ccagcgcttc acgttcgacc
    12961 cgcacaccaa tcggctggac ctgatctccg ttcgcatcca tcgcgccgac ggcacggtcg
    13021 aggaaatccc gcttgataac ccgatcgaac agccgcagcc cgcttccggc attttctggc
    13081 cgacggcgca gtacttgttc acgctgccgc acctggcaat cggcgacgcg gtcgaaacgc
    13141 agacggagat gaccgggttc aatgtggcgt atcttggaag ggatcaaggg atcgagggat
    13201 ctagggatca agagggggcc gccgcggtcg cctcagcggc gaaacccaaa tctgagccca
    13261 aagcgccagc gagggcagcc aacgctgccg cccagccgtc cgccgaggag cccggcttca
    13321 acaacctcaa tgccctgggc gtgccgctgc agccgccggt ccccggacac tggcacgacg
    13381 aggtgcactt ctggtcggga tacccgatca tcgagaagcg ctacacggtg cgcgtcccga
    13441 aggacaaacc gctccaggcc gaggtttaca acggcgagct gcgctccgcg gtcctgttcg
    13501 aaggcgacca gctcgtttat acgttcgaga aaacgaacat cgagcctttc aagagcgaac
    13561 cgagcatgga gtcgacaccc aacgtcggca ccaagctcct gtgcgccacg cttgcgacgt
    13621 gggaagagaa agcccgctgg ctgtacgagg taagcgagcc ccaattcgcc tgggatgacg
    13681 ccatcgcggc caaagtcaag gaagtcatta aagactgcaa aaccgacgag gagaagtaca
    13741 ccgcgctcaa tcactgggtg gccgagaata tccgctacgc cggcaccagc cgcggcatgt
    13801 gcgagggcta caccgtgcac cgcagcattg agaccttcca cgaccgctgt ggcgtctgca
    13861 aggacaaggc cggcatgctg gtgacgatgc tgcgtgtggc cggcttcgat tcgtacctga
    13921 cgatgaccat ggctcgccag cgggtggacc gcgttccggc cgaccagttc aaccacgcgg
    13981 tcacgtgctt ccgcgacaag gacggcaacc tcacgctgct ggacccgacc tggatggtga
    14041 agagccgcga caactggtcg acgctcgaac ccctgcagca cgtggtctac ggcatcccgg
    14101 aaggcaagga actctcgcag tcgccctact tcccgcccga ggactgcacg gttagctgga
    14161 agggccaaac cgccatcagc gaggacaacg agctgaccgg cgggctgaac ttctactcca
    14221 ccggcgcgcc cgaaggccgt ctgcgccgcg ctctggccgc atacccggcc gacgagcgcc
    14281 acgaagtctt cgaagccagt ttcgcccgca tcagcccggt gacgaagatc ggcagcctga
    14341 cctgcatgga cccggtcgac ttctccgagc cgctcaagct tcaggccacg tttggggccg
    14401 aggatttcgc actcggcggc ggcaacaagc gttatttcaa gctgccgctg ctgcagacgc
    14461 cgctgggtga ccgcacgctc atggatttgc cgggcaccta cggcctcgaa acgcgcaagt
    14521 acggcgtgcg gctgtgggcg acgcgtctgg tcaaggtcga cgaaacagtg aagctcccgc
    14581 cgggctggaa ggtggtcgag tcgcccaaac cggtgaatat cgacgggccg tcggcgagcc
    14641 tgcagttcga catcgagtcg acgccgggac aggttcacta cacctgcgag ctgcgaatca
    14701 agaagtggca gatccccccg gaggagtacg ccaacttcaa ggaagtgatg gagaagtttg
    14761 atgaactggc ggggccggta atggtatgcg agctggaggg cgcccatgtt gagcgttaaa
    14821 gaagggatca agggatctag ggatcgaggg atcaagagcg cctttgcagt cgctgtattc
    14881 ctgctggccg ctctcgcgct tccgccggtg gcctgggccg acactgctcc gcagccggat
    14941 ggggttattg agctttggga gcagcattgg acgattaatg acgacggctc gaccgtctat
    15001 cgtgagaagc agcacgttcg ccttaacagc gatcgtaccc accgggaatt cgccgatccg
    15061 cgcctgacgt acaacgctga gacggataat ctggagatca ttaccgcgcg cgtcaaaagg
    15121 gccgatggca cttaccgcga gttgccggac tattcccatg tgctcgtgtc accggacatc
    15181 accgccggct ggccggcctt tgccgccatt cgccagcacg tgctggtgat gagcggcatc
    15241 gaaaccggct gcgtcgtcga gttggattac cagattacca gcaagcccgg tgcgcggccc
    15301 gcactggcgg ccgatgtgcg cctcgatcac gcatatcccg tcctcgaacg cgtactgtcc
    15361 atcacaagcc cggccgcggc caacattagg ccttccgtga ttgggcctgg gggcgcgcag
    15421 atcgggacaa aaccggaagt ctcctcgtcg acctccgtga agagcggcct gccctacgaa
    15481 acgaagacgt ggaccttccg caacctgccg gcgcatccgg ccgagccccg gtgcgctccc
    15541 tggcaggttc gctgcccacg ctttgcgttc aacagcggcg gtgattggca tcggtggatg
    15601 cagcgcacga ccagcgacat cgaaagcgcc gcaaacgagt gcgaaattac cgccaaactg
    15661 gcggccgact ggaccaaaga cgtcctacag ccggccgaca aaatgcgcgc tatccaggaa
    15721 aaactggcgg ccgttttcaa cgtcgtggag tttgactccg actggcagcg cggcggtctt
    15781 cgcccggctt cggagacgat ccgcggcggc tacggtctgc cccaggaagc gaccgcggtt
    15841 ctgctgtcgc tggcacgcgc ggccgggctg ccggctcgcg cggccgtgct cgtaaacgat
    15901 gatcgctggc tcgaatccgc tccgcacgac tcgcaggtgg cggcctatgt cgtcctgctc
    15961 gacggcggcc agcagccgga aatctgggat gcccgccacg ggcgcatcgt gcgcgacagc
    16021 cgttgggccg gcacttcgct gctgatcatg ggcgacgcga gcgcgatgcg cacgccgctg
    16081 cccgcctgga cccaggctga tgagagtcgt tgcgtagtcg gcggcacgat caagatgaag
    16141 gcggacggca cttacgccgg cacggtttcg ctggctacgt ccggcttgtt cgtcgctccg
    16201 gaaagcctgc gctcgagcga ggctcagaag agccgcgtca gcgcgcttct cggccgcgtt
    16261 ttgccggagg tcacggtgga gagtttcgcc gtgaaggaac tgagcgataa cgagttcgcc
    16321 gtggaggcga aggtcaagtc gtccaaggcc ctgaagaaag tcggcggcgc gtattggctg
    16381 caaatgccgg cagacgcgcc gtatgcggcg aacgtcacgc tcccgttggc atacagcgag
    16441 cgcaacgaac cggtccgcat caccggccca ttcgtggagc aagtaaagtt gaccgtggag
    16501 tatccggaga actggacggt cgaagctcag ccggtggaag tgaaggcggt caagggcgaa
    16561 tggggcgaag ccgagcagcg catcacgccc ggcgagcagc gcgtgacgat ttcgcgcaat
    16621 acgcgcatcg cgcaacgcga cctcgccccg aaggcgttcc tgaacgcccg cgagccgctg
    16681 aatacgctgc gcaccgaggc cgcccggacg ctggtcttga aaccgtaggt acaaaactgt
    16741 gagagcgaga ccggctgtta accggccaga ccgttaagaa ccggtaacac aggccaatgg
    16801 cagtacgagg ccgacgatcc gctcaagatc gtcggcctct tccacatcta tccagttcac
    16861 tcgctccagc cgccgcaacc aggtgcgttg ctgcttggcc aaatggcgcg tattgatctt
    16921 gatccgctcg atcgcctcat ccagcgacat ccgcccctca aaatgctcga acaactcggc
    16981 gtaccccacc gcctgccgcg cctgatcgct gagcccgcgc gggtcggccc acaaacgccg
    17041 tgcctcctcg accagcccgg ctgcgaccat ccgccgcacc cgctcattaa tccgccgatt
    17101 gttgacttct cgcggccgcc gcaagccgat cagaacccag tcccactccg gccgccggat
    17161 gctctggctg tcccactcgc gctgtagcac gctgatcggc gtgccggtca gccgatgcac
    17221 ttccaacgcc cgctcgatgc gccgcaggtc gttgcgatga atgcggcccg ccgcttcggg
    17281 gtcgatccgc gccagctccg cgtgcagcgc ctcgatgcct tcaacttcgg cccgccgccg
    17341 gatctccgct cgaatggccg ggtcggcgga gggcccggca aacaagccct gatagaagca
    17401 cttgaaatac agcagcgtcc cgccgactgc gatgaccggg cgcccgcgac cgtgtacctc
    17461 ccccaccgcc ctgtccgcca gttcgatgaa tcgggctacg ctgaacggct cccacggctc
    17521 ggccacgtcg atcaaatggt gcggaatcgc ggcccgcgcc tcggcgctcg gcttggccgt
    17581 gccgatgtcc atgccgcggt aaaccttcat cgagtcgatg ctgacgattt ccgcccccag
    17641 ccgcccggcg attgcgcgcg ccaacgtccc cttccccacc gccgtgcagc ccaaaatcgt
    17701 aatgacgcgc ttctccaagc caatcctgcc tataggtgcc atgttcaagc cgccggtgcc
    17761 ataccaggcg gcgcgagcca tgcccaagcc gcgtagcggc gcgggcatgc ccacttacat
    17821 acggttaccc gatattgcac accaggcgcc gaacccgaca ttgcacacac atgcccgcgc
    17881 ttacagcttg ggcatggcac ccaacgcccg gaccttgggc ctttgacccg cacggcctaa
    17941 ccggcgatta tacggtgcgg tctaagtggg tgagcagcat ggccatcgga cctggcgagg
    18001 gcgtgggccg tgccagcctt gaggagagta acgatgagga cgagtttcct attggccgcc
    18061 agcgccgccc tgatcctgac caccctcggc tgcgtcagtg tgaaagcccc cgagcggatc
    18121 gacatcggtg gcagccggcc cgaaccggtg gacaccggcc gcgtcccgcc catcgcgacg
    18181 gtcgagcagg ggcggcagga gctgtaccgg gcctaccaga acattcagta cttggagcag
    18241 caaaactgcc gcctgaagga gaaggccgac aagtacaagc gcgagcgcga cgaatgccgc
    18301 aagcggctgg agagatacga agattgagcg gcccggagga agcgtgatag agtgataagg
    18361 tgatagagtg ataaagtgat aaggacggac cgctgggtcg cgtgtggccc gctcgccgcg
    18421 cgtaaacgct taatccctat cacattatca ccttatcact tggtgcggcc cgcgcttgcg
    18481 cgtcaggctc ggataagagg acagctctcg gatagaagac gactccggag gcaggagcat
    18541 ggccgtaact ttcgtcctcg ggcgagccgg ctcaggcaag acgcgttact gcgtcgaccg
    18601 cctgctgtcg gagctccagc gctccgggga cacgcgccgc ctcgtgctcc tggtccccga
    18661 gcaagccagc ttccagatgg agcgcacgct ggccacgcgt gctcctaacg gcggatatag
    18721 ccgggccgcg gtcctcagct tctcgcgcct gacgcaccag gccctcgctg agctgggcga
    18781 cacacccgaa gtggtcacac cggcggcgcg cgtcatggcc ctgcgcgccg tcaccacccg
    18841 catgggcgaa gggctacgct atttccgcca cgtcgctcag acagccggtt tcttcacgca
    18901 gctcgagcgc ctggtcgagg aactcctgag cgaggcggtt tcgcccgaga gcctggccga
    18961 agcggtcggc gctctcgatc ctcccgccgc ccgccgcaaa atcgccgagc tgtctgccat
    19021 ttatcagggt tacctggagt ggctcgggcg cgagcgcatc gacccggccg cccgcctcag
    19081 tctgctgcgc tcgcgcctgg cgggcctgac gtggctgcgc gactctttgt tctgggtgga
    19141 tggcttcgcc ggcttcaccg ggcaggagct tgccacgctc gtggagctgg cccgcgccgc
    19201 gcgcggcctg acgctcacgt tgctgcttga cccggactcg ccggtcatcc gcgccaatgc
    19261 agcgcccggc atgttagacc tcttccggcg aaccgaactg acgtatcaga agctgcgaac
    19321 gctcttcgag tcggccggcg tcccttgcac cgacccgatc atcctgcacg ccgcgccgct
    19381 gccgcgtttt gcccaggccc ctcagcttgc acggctcgaa gccggcctgg ccgcaccgtt
    19441 cgcggcaccg gccagccccg cgtccgcaag cgcaacgtca cccgatcaga ctgtttcact
    19501 gattgagtgc gtcacgcacc gcgaggaact tcggcaggcg gcgcgctcca ttcgccagca
    19561 gatcattgaa tccggcggcg ccctgcgttt ccgcgacttt gccgtcattg cccgcgacct
    19621 ggagccgctg gtcccgatca tcgccgatgt attcactgaa tacgaaattc cgtacttcct
    19681 cgaccgccgc cggcccatgc gctctcaccc gctcagccgc ctggtcgagg ctttgttcca
    19741 ggcgatagcc gaggacttct cggtcactgc gaccacccgg ctgctccgca ccggcctgct
    19801 gccgatcgag cgcgagcaag ccgaagccct ggaaaacctg atcgtcggcc aggccattca
    19861 cggcctcgcc gcctggcgcc aaccacactg gaccttcgac gacctgtcga atctggcaat
    19921 taacccccaa cgtgcagcgc cgaacgcctc ccgtgtgcca ctgcttgaaa gcgcagcttc
    19981 aagcagtgcc cgaaccccca atcagcccga ttccgcatca tcgccctcaa gcaccgaacc
    20041 cgcgcccacc gaactcgatt ctgcgcgcct cgccatcatg gccgcaatcg aaccattgct
    20101 aaacctcgcc gcccaggacc caccccccac cgccaacctc tgggtccgca cccttataga
    20161 cctgctagag cagatgtccg tccggcgacg cttggaatcc tggatcgccg ctcaccgcca
    20221 ggagcgccgc tgggaacaag cggagaccca ccgggtggcg tgggaaacgc tatgcggcgc
    20281 tctggacgac ctgcacaccg tcctcggcga cgaacgcctg cgcctcgacg aagtcgcccg
    20341 actgctttcc gcgaccttgc gcgagcagac ccttggcctc gctccgccaa ctttggacca
    20401 ggtgctcgtc agctccatcg agcgcagccg ccaccccgag atcaagttcg cctgggtatg
    20461 cgctttcaac gagggcatct tccccgcccg ccccgcggag gacgaacttc tcgacaccgg
    20521 cgagcgcgac gccctgacga aagccggctt gcccgcgccg gccagccatc gcgaagacag
    20581 cttcggcgag cgtctgctgg cctacatcgc tttcacgcgc cccagcgttg ggctcaccat
    20641 cagctacgcc accgtcgccg aagacggcca gccgctgctg ccctcgccgc tactggcaga
    20701 cgtgcggcgc gtcctgcctg agctgcaaat cgcccgcccg cagccccatc cgccgcctgt
    20761 cagtttggcg gagctggcgc gcggctatct gcacggattt gagggatcga gggatctagg
    20821 gatcaaggga tcaagaagca ctgctgccgg aggctctacg acgagatatg ggaaactggt
    20881 tgcggagcta ctggccgcac ctgggacggg tccccggctg aagcacctgc tgcgcggcct
    20941 ggattaccgc aacgctgccg agtcggtcga tgctacttac cgcacctacc cggcaggaat
    21001 tgcgtggcgc ggttcgccat ctgaggccga gacgtatctc gactgctctt tcaagcattt
    21061 cgcccagtac gcgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctctgcg
    21181 acttcgtggg cgaactcatg cgagcccatg ccggccgctg gcgacgcggg cagtttgaac
    21241 cgcatctcct tgaaagacgt ttcggaagtg agacagaccc ggacggtctg ccggggctcg
    21301 atctgacact gaccgacggg cggcgcgtgc gactacgcgg cgtaatcgac cgcctcgaca
    21361 tctgccgcca ggacggccgg acgctcgtcc tggtttacga ctacaagtcc agcatcagcc
    21421 ccttcagtgg cgccgcgtac cttaccggcg gagcgttgca agttctcacg tacctgctcg
    21481 cgatccgaac cgccctgccg acccgcgacc cgctgacatt ggccggcgtc ttcctcgccc
    21541 cgctcttccc ggatgaagca gtcctgaaaa tacaatacgc ccaggcggcc gaggcggatc
    21601 aggaagccat gtatctctac cgcccacgcg gcgtgttcat cgagagcgcc gcacgcctgc
    21661 ttgatccgca actcgggcag acctcctcgc ccgtggcgtg catgcggctg aaaaaggacc
    21721 tcactttcga tcgcacgcag tcgcgcgacg tggtcgacga aaatggattg gaatcccgcc
    21781 tcgccctggc ccgccagacg ctgctaatgg cggccgaggg catcgtggcg ggcaaggtcg
    21841 aagcctcgcc cttggtcgag cgacacgagc ttgcctgcgg ccactgtgaa tttcacggtc
    21901 tgtgccgctt cgatccgctc atcaaccgcg cccgagccgc cgaacgcgtg ctgcccacgc
    21961 tcgaccagtt cgccgcgcag gtcgaaaccg gcgacacaaa cgaagggggc gccgaatgaa
    22021 cctcactccc agccagcaac aagccatcga ccaccgcggc tgcaatttgc tgctctctgc
    22081 ttcggccggt tccggcaaaa ccgaggtcct cgcccgccgc gtcctggccc tgatcgccga
    22141 cccgcagcac ccctgccccg ttgaacgcct gctggtcgtg acgttcacgc gggccgcggc
    22201 cgccgaattg cgcgtacgta tcggccggat gttgcgcgag gctgcggcga aaacctcgga
    22261 cgcccacatg cgcgaccacc tgcgccggca ggaaatcctg gtcgacagtg ccgatatcgg
    22321 caccatcgac gcgtggtgcg cccgcatcgt gcgcgcccac gccgccgaga tcggcgtaga
    22381 ccccgctttc gtgatgctca gcgaagaaga cgcctggctg ctgcgcagac acgtactgga
    22441 cgagttgttc acgtggattt atttcgcggc cgaccccctg gcgcaagccg cccgcgaatg
    22501 gcttaagcgc acgacccgcc ccgacgacaa gttcctgcgc cggcacgtcg agaaactgaa
    22561 ccagttccgt gaaaacctgg ttgaagtcga cgcctggctg gcccgccagc gcgacctcca
    22621 cgccgccggc cccgacgagc tccgcgcccg cgcttgcgtg acgcttaccg ccgccctggc
    22681 ccaagaatgc gccttccaac accagcaatt gaccgccctg ctcgccggcg cagctcgcga
    22741 actgagcacc gttcttcagc cctaccacga gtccctcgcg gattggaacg cgcgtctcac
    22801 gcaggaaccg caagaggcgg cccttctagc ggtcctagat gagatcgatg cattcaagct
    22861 ccgcaagccg cgcggcctgc cgccggatgc cgccgccctc tgcaccgaca tccagacgcg
    22921 ctggctcaag cgccgcctgc aagcctgctg gtcccgcgac gaggccgtgc gtctgctgga
    22981 cactgcgccg gccgccgcgg cgctcgtgac cacgctgctt gacctcgaac agcgcttcca
    23041 cacgcgcctg agcgagataa aacgctcaca agccacttac gagttcggcg acgtgctgcg
    23101 catggctctc gatctgctgg cgccgccggc cgctaccggc ccgcgcacgc cttccgcgat
    23161 agctcagcgc ctgcagcagc gttacgaaca cgtgctggtg gacgaatatc aggacaccag
    23221 cccggtccag gtcgaaattt tgcgcctggt aacccgcccc cagcccggcc gctcaaaccg
    23281 cttcatggtc ggcgacatca agcagagcat ttacggcttc cgccaggccg agccgcgtct
    23341 cttctccgag ttgctgcagg atttcgcggc cggccgccag gaaggaatcg ttcagtacct
    23401 ttcggacaat ttccgcagcc atcctgcgat cgtcgccgcg ctcaaccgcc tgttcgcaat
    23461 gctatttgac ccggccctcg gcgggacggc cttcgcgccg gacgaacgcc tgcgcgccgg
    23521 acgcgacgag atcgccaacc ccagcctcga tgcgaacccg cgcgttgagc tgcacataat
    23581 tgagcaaccc gccggacgct cagcagccca gaacgaggct gaagattcgg aggacaataa
    23641 cgccgtgccg ctcgagcgca tggagcgcga agcgctgctc gccgccgatc gcatccatgc
    23701 cctgctgcgc gacggcgtgc aggtgcccga gcgcggtccc gatgggcggc tcagcctgcg
    23761 cccgctgcgc ctggcagaca ttgtcatcct cctgcgctcc gcgaagcaga acgccgcagt
    23821 cgtggcccgc attctgcggc aggccggcat cgcttgtatc actagcgggc gcgacgccct
    23881 gctcgacgtc cccgaagtga tggacgtgcg caacgtgctg gccctgctcg ccaatcgccg
    23941 acaggaactc ccgctggccg cctatctccg cagcccgatg gtcggccttt cgctgcccga
    24001 gctgctgcag attcgagcag cggcgccgca tgctgatttc tgcgacgcgg tcgcaacctt
    24061 ctgcacaggc aagccccgcg accaggccgg cgccgccctg caagccaagc tgcgcgccgc
    24121 catggcgcaa ctcgaccgct gggcgataac cgcccgcgaa caggagctgc ccgaactcct
    24181 ccgccagatc tacaacgaca ccgcctggct gctctttgcc caggctctgc ccggcggcga
    24241 gcatcgcgtg gcattgctac gcgccctgga gcgctttgcg atcgacttcg cccgccgggg
    24301 tcagcatggc gtcgccgaat tcgacgccta cctcgaagac ctcgccacgg ccgaactcga
    24361 tcccggcgtt ccggtcgccg ccggcgaaaa cgtcgtgcgc atcatgacca tccacgccgc
    24421 caagggcctc gaattcccgg tcgtcttcct gctcaacacc ggcagccgct tcaatctgag
    24481 ccggaccaac gaaccgctgc aatgcgacgc ctccaccggc ctcggcctga ccttcttcga
    24541 ctacccggcc cgcctgaagg tcaccagcgc ccgccatccg atcgctgccc ggcacggccg
    24601 cacccgtgag atcgaggaag agcttcgcct gctctacgtt gcaacgactc gggctcgcga
    24661 gcagctcatc atttgcgggc atgccaaacc ggacgcgtgg gacaccgctt gcacccagtt
    24721 cgccacggcc ggaggtccgc cgccgctgcc gacgaggttg ggcgctacca gcctgctcga
    24781 ctgggtcatc atggccgccg ccgcggccgg cctgcacgag cgcggcacga acacgtgtgc
    24841 cgcattggag gtcacactgc accagcccca ggaagaaaac gggcgcggga gtctcccctc
    24901 aagcgcgccg cccgaacccc
//
LOCUS       DAOSYS010000018        24901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1646_length_24901_cov_4.817637, whole genome shotgun sequence.
ACCESSION   DAOSYS010000018 DAOSYS010000000
VERSION     DAOSYS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 24901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24901
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1646_length_24901_cov_4.817637"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /gene="gcvT"
                     /locus_tag="PLU99_02120"
     CDS             <1..1147
                     /gene="gcvT"
                     /locus_tag="PLU99_02120"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOO15880.1"
                     /translation="GRRAPTRTDYANCNGDRMLKTPLNEFHRQHGGRMVNFAGWEMPV
                     VFTGILEEHQYTRQHWTMFDVSHMGRVEFRGPGAAAFLQKLVTRNVDKLAVGQCGYSH
                     ICREDGGILDDLIVSRYDDYFLVVCNASNREKLLGWFDRQRAGFDVQIKDRTFETAMV
                     ALQGPDTFDALAKLLPVSLEGLKRYHFRCGTVMGVEASIARSGYTGEEGVEITMPANL
                     AMMAVNVLFERAEQMGRPLKPAGLGARDTLRLEAAMPLYGHELTEDWDPITAGQTWCV
                     DLTKDFIGQPALKRIAEEGPKRQIVGFEVSGPRIARQGYPVLADGRQVGFVTSGVQAP
                     SVQKNIAMGMVDAAHAAPGTTVEIDLRGTKTEAKIVPLPFYKRPAKK"
     gene            complement(1393..4191)
                     /locus_tag="PLU99_02125"
     CDS             complement(1393..4191)
                     /locus_tag="PLU99_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOO15881.1"
                     /translation="MIRHWWQLGVRNWHVKRVRTLGALLAIALGVGAVVWVTCCYESV
                     RETVMGWASGYVGRSHINIESPFGKFSQFAQRRVAEVADLPEVKSVTPLLVERLRGKA
                     VRRADFVEGTKYDPWSSPEIDFTGIDLDSEPLVRDHPIVAGRGLTAADHYAVVLEASY
                     AREEGVGPGDYLIIWNESGDTHQKLEIVGLTERRRIARFQPPLALLRLPVLQQIDNKY
                     GFITSLDVILHDGSRTALAQATAKIRRIVLANNAGAHVRSAEARLKQIELAQQQQEVV
                     LALLSCVAMLTALFIILSTLSMGMLERISQLGLLRCVGVTASQLAALVYLEVLPLGLL
                     GIALGIPLGLGLTGLTVKAVPEYVGRFAISWDGIGLAAIAGFATTLIAATLPAMAAIS
                     VSPLEASRPRARRPRRAPLIAATLLGVGLLVAQVAIMAFKIGRNFEFVRWSAASVVLL
                     YCVYALAAPLMVWLLGSPAVLLIARLVGVRARLLQDQVGHAVWRSAGIVCGLMVGLSL
                     IVALVVLNRSFRAGWQFPKQFPEAYIWSYEQFGITPEEAARRIEQFAGVKNFALANAL
                     NMAVEERQMQGPRAQIFHSATWFLGCDPDTFLDMVKFEFIEGDEETARELLRQGGHVL
                     VSIDFARSRHKGLVPDEARGISNTVRVWFSDLGARTFKIAGVIDSPALDIAASFFQAE
                     SEARVAAMGSMIGSNADLKRFFNIDTIKLVLLNFDLPPEPPPPNWPPPPEQAGPELRA
                     SLNTDRKWWYDDDVPLAVRWQRAREHELLLELRNALDAHSAYVGTASELKDHIDAELT
                     RVTYLLTAIPAVALLVAAIGVANLMTANVASRSKQLAILRAVGATRGQILRMVVGEAL
                     VLAAIGSALGLALGLHLAKNVQQMTLRFSGFIGPLEIPWAFVGLAVALTISLCLLAGI
                     LPARHASRTNVIEALHVA"
     gene            4514..5596
                     /locus_tag="PLU99_02130"
     CDS             4514..5596
                     /locus_tag="PLU99_02130"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HOO15882.1"
                     /translation="MDQPANSTARTSGRKSPRGEYVCTPAFLRSLDGFRVYQNVELGL
                     SANTIEAYRRDLCRLGDFLRRSGIDDWEQITPQALQPYFIELADANYRETTIARHVVA
                     IRMWLRWLVISRQVSTDRTSLVELPKRWQRLPKTLNLERMAELVVSPDLEEELGLRDR
                     AILELFYACGLRVSELCGLKERDVDLSAGYARCMGKGRRERVVPVGREARAAVREYLE
                     KLRPRLVARGVERGRFKTPLTRAVAAAIPLFLSRTGGPIERTAVWRLVRREAKRCGIE
                     KQVSPHTLRHTFATHLLEGGADLRVVQELLGHASISTTEIYTHVQVKHLKEIHARCHP
                     HGALQEEERKEERRRGLPAKPGPARP"
     gene            complement(5723..6499)
                     /locus_tag="PLU99_02135"
     CDS             complement(5723..6499)
                     /locus_tag="PLU99_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15883.1"
                     /translation="MNLRRRLTQESAAQVFGDAVRERALAVLSVQCGSDWRMFKSRFL
                     ESDSNQAFFVLDYQPVNEQPLPELWPGQCVGISFRSRNRKVMFATVVEARGHYMLESG
                     SVPAVRYRWPQAITELQRRAYYRTPVPAGIVLAATMWRGGLAARTAVQSGSQLISGTL
                     ANISCGGALVRLNQPAAIDWVEGELLGLEVQLGDRQGPAQLDSYFRGLRHDEASHSGV
                     ALQFIGLELNVEGRLVLERLANVVQKFHRQAITAGARLWQ"
     gene            complement(6496..7227)
                     /locus_tag="PLU99_02140"
     CDS             complement(6496..7227)
                     /locus_tag="PLU99_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO15884.1"
                     /translation="MQGLLDLTHRQAVRVLEQALRTRARLELEPSGGDWSVSGYLAGR
                     EGDLLRVDLHDRGRDWPLTPLAGALCEVRMILDGQMYRFSSCILTAEAAGAAQRLMLS
                     APGVVQVCNRRRYDRRSCPEAPVIQVFTQNQPGALVGTISEIGLGGLACLLPGAADEE
                     LLIDEIVRLRFELPGGDETYELSACVCLKNAPQTGDDMIVGLEFCPDTSGNGALGRLR
                     QALAQEYTGRCTGGHEGHDTAEDEP"
     gene            complement(8079..8432)
                     /locus_tag="PLU99_02145"
     CDS             complement(8079..8432)
                     /locus_tag="PLU99_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOO15885.1"
                     /translation="MFEKLQVYQKAVDLADEIASLTEAFPRGYGFLVDQLNRAAVSIA
                     TNLAEGNGRFTVPDRRNFFTIARGSAQECVPLTEIARRRGLIQEAASLALRERLEIIA
                     KMVSGLINGLDQRKS"
     gene            complement(8680..8754)
                     /locus_tag="PLU99_02150"
     tRNA            complement(8680..8754)
                     /locus_tag="PLU99_02150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8717..8719),aa:Pro,seq:ggg)
     gene            complement(8779..9438)
                     /locus_tag="PLU99_02155"
     CDS             complement(8779..9438)
                     /locus_tag="PLU99_02155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOO15886.1"
                     /translation="MAILGLALDLGSKSWAFTTLRQSGRRVIIPHVLHFETALNDGAL
                     FGIGSGRTGLFLLASVLALVLVLWMFTQTSARSWLVQLALGAVLAGALGNMYDRMFVR
                     LVGVSEAGAPRYYEKVADPDDGSVRLREYPPLPNDPGRAVPPAVAAQLAEVGYVRDFI
                     KIPVKFYRKQVWPWVFNVADSLLVCGVAILALKLWRERERGGEKRGLPGEKLDPQPQK
                     A"
     gene            complement(9609..10226)
                     /locus_tag="PLU99_02160"
     CDS             complement(9609..10226)
                     /locus_tag="PLU99_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOO15887.1"
                     /translation="MAKKPVNKNVSKKTSRGGSSAGSARARKAPAGNGRATRAASAKK
                     RAPAGRVQPAPKAVVQRVSTPDEGAGQVRTSAEAGLTKKDLEQFREMLLQKRAELLGD
                     VHTLRKEALKGSRQEVAGDLSSMPIHMADLGTDNYELEFTLGLIEGGRAVLQEIDEAL
                     ERIERGTYGICQATGKPIGKARLRAKPWAKYSYEYVLAQETGKRA"
     gene            complement(10469..11653)
                     /gene="galK"
                     /locus_tag="PLU99_02165"
     CDS             complement(10469..11653)
                     /gene="galK"
                     /locus_tag="PLU99_02165"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOO15888.1"
                     /translation="MQRDELRAAFAARFGYACRAVARAPGRVNLIGEHTDYNEGFVLP
                     IATPHATWVAVGAPPAGRAAERDPEIRACSATLDDMQTWAAGKWSRAGAPHWTSYIAG
                     VAEMLRRRGLPVAGFDLLIDSDLPLGAGLSSSAALSVATALGLASLVGASLSREEIAP
                     ICRAAEHEFAGVPCGVMDQYASLFCQENHALLLDCRNLQIRQVPLRLDGHTFLVINSG
                     VKHELASSEYAKRQEQCAAAVTHFRQADPAIRSLRDVTESQVAAEAERMPDEVAARAR
                     HVTSENRRTLSATEALERGDVAHFGRLMYESHLSLQLDYEVSCAELDLLIELLAGVPG
                     VLGARMTGGGFGGSVVALAQADAVDPVRRELHQRYDTTDRQAALILVRPSAGASVTCG
                     EQ"
     gene            complement(11662..12669)
                     /locus_tag="PLU99_02170"
     CDS             complement(11662..12669)
                     /locus_tag="PLU99_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15889.1"
                     /translation="MAIIPPPFRGVRLFGKIALIVSTLACLTSMGLGLFELTQNDHLW
                     GLALVAAGLMLLAVTILMYAQLTLAHRLMGDTHRTYGALLDITELLRRQTDYTHTIAD
                     NSSLSDWAKRVVYREKDYEYLHDTIKSAIVRQDWEGAEQFIRDLDQELGLREEAARFR
                     QEVEQARRATTEEKIAAALKRFDLLCEQHKWDQARSETTRLKTLFPDEPRIEALSREV
                     ELRRQQVKRKLLKDYDEAVRAQNVDLAHQLLLELDKYLGVDEVAVLKESARGVFKARL
                     FQMGVEFSLAVTDRNFPRAISVGERIVREYPNSRYAQEITSMMPALRERAERESPQHA
                     V"
     gene            complement(12876..13715)
                     /locus_tag="PLU99_02175"
     CDS             complement(12876..13715)
                     /locus_tag="PLU99_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15890.1"
                     /translation="MIRSFAVTLVCFLAVAALGQTTTTTTDPQSQTPTENLAQAAAHG
                     PASWVTAARQRHQAFIEARVTNPRQGELAGTGDGLEPNPATGTVGTGGLGGLGDLGGL
                     LDLVGGAGGISDLLGGFSGGTGPAGTGGMTIQDLLALRDQLLGESGGSSTSGTSATSG
                     KSGADNGKTANRSMELRGGSGAIGRLPKIDDRSATTPTALADTTTTGTTQQPKFMARL
                     ATGLADAFFGAVSFGFQSDAFVSYVKAWMRPMFFPQEYGGSESADDDTDETDSDSDSG
                     SII"
     gene            13957..14901
                     /locus_tag="PLU99_02180"
     CDS             13957..14901
                     /locus_tag="PLU99_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15891.1"
                     /translation="MIGFIGPPATDAISAAVCGGAERFAAAYPNVHCLTVIPPDAQPS
                     SLSAAVDETLARNPNAICLYLTSTRAPNDADHTAQLIERIRQTGIVLVTVGSPINNPA
                     VRGHVEVALPQAAEILAQNLRSLLPPAVNAFGHVRQERSYALLHADGRDQSASDTYAR
                     FLATVRDNSNLARLADVNTWASDRSAQRLIADTLERFSTVELIVTLDPEPWLSLRPRL
                     RLPPRNHFATLSAAPRLWPRLESGEALALVGPLDGQIGYTAMEVATQRLMLVPDAPTR
                     RYIPCELVTKANLDDFAQRYAAAANLDVRDLLSFRPLP"
     gene            15047..>15540
                     /locus_tag="PLU99_02185"
     CDS             15047..>15540
                     /locus_tag="PLU99_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO15892.1"
                     /translation="MAWVLMTLLLVLSWGIFAYSAVRRVRLMMVAQAPDNRCDHLGER
                     IKSVLVYMFGQKRMPRYPLAGWAHIAVFFGFMVLLLNSLILWARPYFGPHFAFGLFGL
                     HQPLGVAYAFLRDIFTVLVIIGVLIFYWNRLVVRLGRLTLNLEGVLILTIIFVMMVAD
                     LVYEG"
     assembly_gap    15541..15640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15641..17125
                     /locus_tag="PLU99_02190"
     CDS             <15641..17125
                     /locus_tag="PLU99_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO15893.1"
                     /translation="GRVPPIQDIEGRLEREETLGAKRIRDFSWKAALDFYTCTECGRC
                     SDQCPATRTGKLLSPKHLCIALRDHMYLREEQLIGPRFSLKRKPEDQSKTDSTEQGAV
                     EVEAPGKPHGTRDHQRLLHDPAAPAVLHTEQLTPAWVNPEVLWACTTCGACEQECPVF
                     ITYIDKIVDLRRNLVMERGEFPEQLQATFKGLETVGNPYSFSPEQRAEWAQGLNIPLR
                     AEKPDAEWLFWVGCAPSFDDRSRKIARATAELLQEAGVDFAILGPEEQCNGDPARRAG
                     NEYLFQMLAQANIETLNRYNVKKIVTTCPHCYHTLKNEYPDFGGHYEVTHHAELLAQL
                     IQQGKLRPRFPLKATIAYHDACYLGRHNQIYDAPREIINQIPGAYVAEAPESRDRGMC
                     CGAGGAQMWKEEEHGDARVNHTRVKQLLRVIPGGGTSTTPAGTKPTIATACPFCMTML
                     TDGCRDQGFDDVQQLDVAEILHRAVKGPKETQNAAEASAAQPAR"
     gene            17377..19362
                     /locus_tag="PLU99_02195"
     CDS             17377..19362
                     /locus_tag="PLU99_02195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOO15894.1"
                     /translation="MMMSAAGLPFAHADQTPLIPRQTLFGNPQRASVQISPNGQYLAY
                     LAPVDGVLNVWVGPLGDPAAAKPITQDKGRGIRIYFWAFDGRHVLYLQDKDGDENWRV
                     YSVDVDRGDIRDLTPLENVQARIQNLSHRKPNEILIALNDRNPELHDIYRLDLTTGER
                     QLVLENEGFMAFHADEDYQIRLAERTTPDGGREIFQRSTDGTWQPFIQLAPEDALSTN
                     VAGFDKTGRVLRLIDSRGRNTAAFTEIRLDDGKQTLIAEDPRADVSGAMAHPTEFTLE
                     AVSFTYERVHWKVLDPKMEPDFAALRAVADGDFEITSRTLDDTKWIVAYVLDNGPVRY
                     YLYERPARKAKFLFTNRPELEKLTLARMHPVIIKSRDGLDLVSYLSLPPESDPDNDGR
                     PDRPLPMVLDVHGGPWARDEWGYDAYHQWLANRGYAVLSVNFRGSTGFGKEFTNAGDR
                     QWAAKMQDDLVDAVQWAIRERVADPQKVAILGGSYGGYAVLVGLTFTPELFACGVDIV
                     GPSSLVTLLESIPPYWKPILDLFAARVGDPRTEEGRKFLLERSPITRVDQIRRPLLIG
                     QGANDPRVKQAESDQIVAAMQEKNIPVTYVLFADEGHGFARPENNMAFNAVTEAFLAR
                     QLGGRFEPIGNDFQGSTIQVPVGADQVPGLAEALKQQ"
     assembly_gap    19371..19470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19483..20559
                     /locus_tag="PLU99_02200"
     CDS             19483..20559
                     /locus_tag="PLU99_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15895.1"
                     /translation="MKRTLWGLPVFLAGAVMVLAAQQPPATPAEPDDGLDALRAKDLL
                     GDDDRAALRAWIGERIAMVIGDDVAGAQQAVAQLRQGARGGRGFTDAYVAATMESIGS
                     AYKKAELVPASRLLIVLGELNDPRTFNTLLGALQDERTGVRAAAAVGLRKLQSKLDPA
                     AMNEMLAALRAAGEKETAPRTLDLIYRAMNLTEAPGLTERRASSTAIVDLLQQRLKSI
                     ENGRMRAEGGDLAALEALDPQRGNLDDATRRRYIAELGRLMHYCVCRYVRDDPLSRVR
                     DQSSSPDVVELRNNIEVTIREAERQLQNLVGAPEGANIAENMTRAKITEMAIAMNKWS
                     EKLKGVDLDLPEVQRAPGDEPGGL"
     gene            complement(20586..20849)
                     /locus_tag="PLU99_02205"
     CDS             complement(20586..20849)
                     /locus_tag="PLU99_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO15896.1"
                     /translation="MSHYITQPCIGVKDTACVEVCPVDCIHPRKDEPDFEAAEQLYID
                     PDTCIDCGLCVDECPVKAIFPEDEVPEKWRDFIERNAQYYRRS"
     gene            complement(21061..22359)
                     /locus_tag="PLU99_02210"
     CDS             complement(21061..22359)
                     /locus_tag="PLU99_02210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO15897.1"
                     /translation="MSDEFHLRVQELLERALSLDSAQRQAYLKRECGRNDLLLQEVLS
                     LLPHYEQMRAFELERPQKTALGMPGTTTYGQIAVAVEQEPPELEPELPFALGQYRVVQ
                     VLGRGGMGVVYRAVYPTLRKEFAIKVLRKDLVSYEDRWRFAFEAHILRQLRHPGIAQI
                     THASEISTTSGPQPYFVLEYIEGESLIRYAESHKLDVDARLGLFCRVCEAVEYAHWHG
                     VIHRDLKPSNILVDTTGQPKVLDFGIARLAELQADALRNERGRFVGTPEYASPEQTRG
                     LTEQLTPRSDVYSLGLIAHELLTGRRPRLDQGRLHLDVSRVCPTDKRGREPSNLGEFH
                     YFLKAVLCNALAHDEAQRYATAGELGADMEALRGMIAPPRGLAALTSRLRDLICPRSQ
                     WSPSSASRPLSAVLRKRVSMALEAEDHYLQQGRPAPHSGS"
     gene            complement(22349..>22918)
                     /locus_tag="PLU99_02215"
     CDS             complement(22349..>22918)
                     /locus_tag="PLU99_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO15898.1"
                     /translation="VPLVYQELRQVARRYFRNQRPGFTLRPTEVVDEACLHLIQHSRI
                     EWESSEHFRAIATRKIWQVIVDHLKKRYARKRGGVPLASATASEEAAEEPGAADDTPW
                     QRVPLEGISVEWRDRTVDVLDLADALDALAAESARLKDVVMLHWFGGLKYSEVARCLG
                     VSASTVEKDFRYALAWLNRRLTGNSEDVR"
     assembly_gap    22919..23018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23213..24004)
                     /locus_tag="PLU99_02220"
     CDS             complement(23213..24004)
                     /locus_tag="PLU99_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOO15899.1"
                     /translation="MHLRLLTYNIHKAIGIDGKFAPQRIVEVLGHYQADVLLLQEVDR
                     GVPRSGMLDLAAFLGRELGYAYRAVGMNVFMRCGKYGNATLSRYPIGRQHNLDLTIGG
                     RKRRGAQHSRIHVVRNGQQAEIDVFNIHLSLMALLRHHQMRRLLAFIAAFDSASTPCV
                     VAGDMNDWQGMLKRRFFVPAGFCCATNRRPGSRWAIKTFPSIAPTGGLDKIFYRGPLR
                     PVQVRRSHLKLARVASDHLPVIADFELSEGPAASATHLGRAAEPF"
BASE COUNT         4492 a         8227 c         7481 g         4401 t
ORIGIN      
        1 tggacgccgc gccccgacgc gaaccgacta cgcgaactgc aatggagatc gcatgctcaa
       61 gaccccgctg aatgagttcc atcgccagca tggcggccga atggttaatt ttgcaggctg
      121 ggaaatgccg gtggtcttca ccgggatcct cgaagagcac caatacaccc gccagcactg
      181 gaccatgttc gacgtttcgc acatgggccg cgtggagttt cgcgggcccg gggcggcggc
      241 tttcctacag aagctcgtga cacgcaacgt cgataagctc gccgtcggcc agtgcggcta
      301 cagccacatt tgccgcgagg acggcggcat tctcgacgac ctgatcgtct cacgctacga
      361 cgattacttt ctagtggttt gcaacgcttc gaaccgcgag aaactgctgg gctggttcga
      421 tcgccagcgg gccggcttcg acgtgcagat caaagaccgc accttcgaga cggcaatggt
      481 cgcgctccag ggcccggaca cttttgacgc cctggccaag ctcctgccgg tctcgctcga
      541 ggggctgaag cgctatcact tccgctgcgg cacggtcatg ggtgttgagg cctcgatcgc
      601 ccgcagcggc tataccggtg aggaaggcgt cgagatcacc atgcccgcca atctcgccat
      661 gatggcagtc aacgtattat tcgaacgcgc cgagcagatg ggccgcccgc tcaagccggc
      721 cggactcggg gcacgcgaca cgctccgtct ggaggccgcc atgcccctgt acgggcacga
      781 gctgaccgaa gactgggacc cgatcacggc cggccagact tggtgcgtcg atctgactaa
      841 agacttcatc ggccagccgg cgctcaagcg catcgccgag gaaggcccca agcgacagat
      901 cgtgggcttc gaggtttcgg gcccgcgcat cgcgcgccag ggttacccgg ttctagcgga
      961 cggccggcag gttgggtttg taaccagcgg cgtacaggcg cccagcgtgc agaagaacat
     1021 cgcgatgggc atggtggatg ccgcacacgc ggcccccggc acgacggtcg aaatcgacct
     1081 gcgcggcacg aagaccgagg ccaagatcgt tccgctcccg ttctacaagc gccccgcaaa
     1141 gaagtagcag agccgggggc gtgccgcact gggatcgagc tagcgcccgc gatgacgtgg
     1201 taaagtgata aagtggtagg gtgataaggg acgatccacg aaaccccact ctatcacctt
     1261 atcactttat cactccggtg cccacgatga acgtgataag gtgctaaggt gatagggtga
     1321 taaaggaacg cccgcgaaat ccccttatca ctctatcact ttatcatcgg gcgatcatcg
     1381 ggcggattca catcacgcca cgtgcaatgc ctcgatcaca ttcgtccgcg atgcgtgtcg
     1441 cgccggcagg atgcccgcca gcaggcacag gctgattgtc agggcgaccg ccagacccac
     1501 gaacgcccac ggaatttcca gcgggccgat gaagccggaa aagcgcagcg tcatctgctg
     1561 cacgttcttg gccaggtgca ggcccaacgc cagccccagt gcgctgccga tcgccgccag
     1621 aaccagcgct tcacccacca ccatccgcag aatctgcccg cgtgtggcgc cgaccgcacg
     1681 cagaatcgcg agctgcttgc tgcggctggc gacgttggcc gtcatcaggt tcgccacgcc
     1741 gatggcggcc accagcagcg cgaccgccgg gatggcggtc aataggtacg tcacgcgggt
     1801 caactcggcg tcaatgtggt ccttcagctc gctggccgta ccgacgtaag cgctgtgcgc
     1861 atcgagtgca ttgcgcaact ccagcagcag ctcgtgttcg cgggcgcgct gccagcggac
     1921 cgccaacggt acgtcatcgt cgtaccacca ctttctgtcg gtgttgagcg acgcgcgcag
     1981 ctcggggccg gcctgctccg gtggcggcgg ccagttcggc ggcggcggct ccggcggcag
     2041 gtcgaagttc agcagaacca acttgatcgt gtctatattg aagaagcgct tcagatcggc
     2101 gttgctgccg atcatcgagc ccatggccgc cacgcgggcc tcgctctcgg cctgaaagaa
     2161 gctggccgct atgtcgagcg ccggcgaatc gatcacgccg gctatcttga aggtgcgggc
     2221 cccgagatcg gagaaccaga cccgcacggt gttgctgata ccccgcgcct cgtcggggac
     2281 caggccctta tggcggctgc gggcgaagtc gatggaaacc aacacgtgcc cgccttgccg
     2341 cagcaactcg cgggccgttt cctcgtcccc ctcgatgaac tcgaatttca ccatgtcgag
     2401 gaacgtgtcc gggtcgcagc ccaaaaacca ggtggccgag tggaagatct gtgcccgcgg
     2461 cccctgcatc tgccgctcct cgaccgccat attcagcgcg ttggccaagg cgaagttctt
     2521 cacgcccgcg aattgctcga tgcggcgcgc ggcttcctcc ggggtgatgc cgaattgctc
     2581 gtagctccag atataggcct cagggaactg cttgggaaac tgccagccgg cgcggaagct
     2641 gcgattgagc accaccaggg ctacgatgag cgacagcccg accatcagcc cgcaaacaat
     2701 gccggccgaa cgccacacgg cgtgcccgac ctggtcttgc agcagacggg cgcgcacgcc
     2761 caccagacgc gcaatcagca ggacggccgg cgagcccagc agccagacca tcagcggcgc
     2821 cgccagggca tagacgcaat acagcagtac gacggacgcc gccgaccagc gcacgaactc
     2881 gaaattgcgg ccgatcttga aggccataat cgccacttgc gccacaagca gcccaacgcc
     2941 tagcaacgtg gcggcgatga gcggcgcgcg gcgcgggcgg cgggcgcgcg gacgtgacgc
     3001 ctccagcgga gagacgctga tggccgccat agccggcaac gtcgccgcga tcagcgtcgt
     3061 ggcaaacccg gcgatggccg ccaggccgat gccgtcccaa ctgatggcga agcggccgac
     3121 gtactcgggc accgctttga ctgtgagccc ggtcaagccc aggcccaaag gaatgcccag
     3181 cgcgatcccc agcagcccga gcggcagaac ttccaggtaa accagcgccg cgagctggct
     3241 ggccgtcacg cccacgcagc gcagcagccc gagctgcgag atgcgctcga gcatgcccat
     3301 gcttagcgtg ctcaggatga tgaacagcgc ggtgagcatc gccacgcaac tcagtagcgc
     3361 gagaaccact tcttgctgct gctgtgccag ctcgatttgc ttgagacgcg cctccgcact
     3421 gcgcacgtgc gcgccggcat tgttggcgag cactatgcgg cgaattttgg cggtcgcctg
     3481 cgccaatgcc gtgcggctgc catcgtgcag aattacgtcg agcgaggtta tgaagccgta
     3541 cttgttgtca atctgctgca ggaccggcaa acgcagcaac gccagcggcg gctgaaaccg
     3601 ggcgatgcgg cggcgctcgg tcaggccgac gatttcaagc ttctggtgag tgtcgccgga
     3661 ttcgttccag atgatcagat agtcgcccgg gccgacgccc tcctcccggg cgtagctggc
     3721 ttcaagcacg accgcatagt ggtcggcggc cgtcaggccg cgcccggcga ctatggggtg
     3781 gtcacggacc agcggctcgc tatcgaggtc gataccggtg aagtcgattt cgggtgagct
     3841 ccaggggtcg tacttggtgc cttcgacgaa gtcggcgcgg cgcacggcct tgccgcgcag
     3901 acgttcgacc agtagcggcg tgaccgattt cacttccggc aaatcggcca cctccgctac
     3961 ccgccgctgc gcgaattggc tgaacttgcc gaacggcgat tcaatgttga tgtggctgcg
     4021 gccgacgtat ccggaggccc agcccatgac ggtctcgcgg accgactcgt aacagcacgt
     4081 gacccacacc acggccccca cgcccagcgc gatcgccagc agcgcgccga gcgtgcggac
     4141 gcgcttgacg tgccaattcc tgacgcccag ttgccaccag tgcctaatca agctttttga
     4201 ttctaacgag atctcgaggg cggtacacga ttcggccggt tggcgacact cgccaatgtg
     4261 gatgtccggc ccaaaggcgt agcgtcggcc ccccgtggcc gacgtcgatg tccaacccgc
     4321 acgcgtaaca tcggcccccg tggccggcgt gtcctgcccc gcgcggcggc gtccgttcat
     4381 cggcgttccg ctattcctat gtttgcggct ggcaaggatt ttgggccccg tccggggcga
     4441 cacgttgccg ctcggctctt aggatgtacc ggggcggtct gcggcgccgc gtgtcgcatg
     4501 gaggcgagcg gaattggatc agccggcgaa ttcaacagcg cgcaccagcg gtaggaagtc
     4561 gccgcgaggg gagtacgttt gcacccccgc cttcctgcgc tcgctggacg gctttcgcgt
     4621 gtaccagaac gtagagctcg ggctgtccgc caatacgatc gaggcctacc ggcgcgactt
     4681 gtgccggctg ggggattttc tgcggcggtc cggaatcgac gattgggagc aaatcactcc
     4741 ccaggcgctg cagccgtact tcatcgagtt ggcggatgcc aactaccggg aaaccacgat
     4801 cgcccggcac gtcgtggcga ttcgcatgtg gctgcgctgg ctggtcatca gccggcaagt
     4861 gagcaccgac cgaacgagcc tggtagagct gccgaagcgc tggcaacgat tgccgaagac
     4921 gctcaatctg gagcgtatgg ccgaactggt cgtgtcgccg gacctggaag aagaactcgg
     4981 cttacgcgac cgggccatac tcgagctgtt ctacgcttgc ggattgcgcg tgagcgagct
     5041 gtgcgggctg aaggagcggg atgttgactt gtcggccgga tacgcgcgct gcatgggcaa
     5101 ggggcggcgc gagcgcgtcg taccagttgg gcgagaagca cgggccgccg tgcgcgagta
     5161 tctggaaaag ctacggccac ggctggtggc gcgcggcgtg gagcggggac ggttcaagac
     5221 gccgctgacg cgcgcggtcg cggcggccat tcccctgttc ttaagccgta ccggcgggcc
     5281 gatcgagcgg acggctgtct ggcggctggt gcggcgcgag gcgaagcgct gcgggattga
     5341 aaagcaggtc agcccccata ccttgcggca taccttcgcc acgcacttgc tcgaaggtgg
     5401 ggcggatttg cgtgtcgttc aggaactgct cggccacgcc agcatcagca caaccgagat
     5461 ctatacgcac gtacaggtca agcatctcaa ggaaatacat gcgcgctgtc atccgcacgg
     5521 cgcgctacag gaggaggagc gcaaggaaga gcgccgacgc ggtttgccgg cgaaacccgg
     5581 cccggcgagg ccttagtgcg gaccgcgccc gcatgccgtc tgcacgtgca gttagcggtc
     5641 agacgggctg ccggtccgca actttagttc gacctctaag cagtccgagc cggccccgcc
     5701 tgctcttggc ggcggccgca ctctactgcc acaaccgagc gccggcggtg atggcttggc
     5761 gatgaaactt ctgcacgacg ttcgccagcc gctccagaac gagccggccc tctacgttca
     5821 gttcgagccc gataaactgc aatgccacgc ccgagtggct ggcctcgtcg tgccgcaagc
     5881 cgcgaaaata cgaatccagt tgcgccgggc cttgccggtc cccgagctga acttcgagcc
     5941 ccagcagttc gccctcgacc cagtcgatcg ctgccggctg attcaaccga accagcgcgc
     6001 cgccgcacga aatattggcc aacgtgccgg aaatgagctg gctgccgctc tgcacggccg
     6061 tgcgcgccgc cagtccgccg cgccacatgg tggccgccaa aactatgccc gccggcaccg
     6121 gcgtgcggta gtacgcccgc cgctgaagct cggtgatcgc ctgcggccaa cggtagcgca
     6181 cggccggcac actgccgctt tcgagcatat agtgcccgcg cgcctcgacc accgtcgcga
     6241 acattacctt gcggttgcgg ctgcggaagc tgatgcccac gcattgtccc ggccacaact
     6301 cgggcagtgg ttgctcattt accggctggt agtcgagcac gaagaaagcc tggttgctgt
     6361 cagactccag gaagcgcgat ttgaacatgc gccagtcgga tccgcactgt acggaaagca
     6421 ccgccaaggc gcgttcgcgc accgcgtccc cgaatacctg ggccgccgat tcttgggtta
     6481 gccgccgcct taggttcatg gctcatcctc tgctgtgtcg tgcccctcgt gccccccggt
     6541 gcagcgtccc gtgtattcct gcgccaacgc ctgccgcagc cgccccagag ctccgttgcc
     6601 cgaagtatcc gggcaaaact cgaggccgac gatcatgtcg tcgccggtct gcggcgcgtt
     6661 tttgagacag acgcacgctg acagctcgta ggtttcgtcc ccgcccggaa gctcaaaccg
     6721 caggcgtaca atttcgtcta tcagcaattc ctcgtccgcc gcgcccggca gcaggcacgc
     6781 caggccgccc agcccgatct cgcttatggt cccaaccagc gccccgggtt ggttctgcgt
     6841 gaagacctga ataaccggcg cctcagggca tgagcggcgg tcgtagcgcc ggcgattgca
     6901 gacctgcacc acccccggag ccgatagcat caggcgctgc gccgcaccgg ccgcctccgc
     6961 cgtcaggatg caactcgaaa agcggtacat ctgcccgtcc agaatcatgc ggacttcgca
     7021 aagggccccg gccagcggcg tcagcggcca gtcgcggccg cggtcgtgca gatcgacccg
     7081 caacaggtcg ccttcgcggc cggccagata gccgctgacc gaccagtctc ctccactggg
     7141 ctcgagttca agccgggctc gcgtgcggag tgcctgctcg agcacgcgaa ccgcctgccg
     7201 atgcgtcagg tcgagtaagc cctgcatacg ccaaacctcc cgcgcgccct atgctcagcg
     7261 cttgcaaaac cgcgccgtca gtcgcgcgct gccacgcagt actagcggac tgctggccct
     7321 caatctcttc tcgcgcgtcc ttctcgggcc gcacacgttc tctcccgcac ccgcaacaac
     7381 tcctctttct tggcgtcctt ggcgctcttg gcgtcttggc ggttgccttg tacctgcgcc
     7441 gtgcgaatct tcgtctcgac tcgcgattta cttgactggc gttggccgac gtccagggcc
     7501 cgaatagccc tccgcgccgt gtcgagtaaa ggccgcgcca gaccaccgac agggctatgg
     7561 gaactaaccc gttcagtccc ggaacatggc gactggaccg cggtgtcgcc tgtcgcggcc
     7621 tcacgatatc ggccgcgcaa gccggatttc ccgtaaattt cacaggtccg ccggaggttt
     7681 atctaatgcg tccctccacc tacccgcgac ggaaaaaacc gcggccaccg gccgcacgtc
     7741 ccttccaccg ctgataatcg ctggttcatc gagtgcagcg ggatttaaaa aatcgctggt
     7801 ttcggcgcca tctctgcagg ccaagtacac gcgccgccgc ctcgctgtcc cgctcgacgt
     7861 gctggtgcgc gtgtgatcca atcggcaacc gtcagtggca cgacatgggg gtccgaacag
     7921 ttccgcgcga gtgcgggcgc ggctttgaac tgaaacggcc gttgctctcg gcgagattgg
     7981 ttgcattggc cggcgtcgca gaggcgctgc cgtctgtgta acgaacgttt ctgtacacaa
     8041 ggcacgacgc tactgcgccc gaggccgccc cggggaagtc acgatttgcg ctgatccagt
     8101 ccgttgatca atccgctcac catcttggcg ataatctcga gtcgctcccg cagagcgagc
     8161 gacgcggcct cttgaattaa cccgcgacgg cgcgcgattt cagtcaaggg gacacactct
     8221 tgcgcagaac ctcgcgcgat cgtgaagaag ttgcggcgat cgggcacggt aaagcgaccg
     8281 ttgccctcgg cgaggtttgt cgcgatcgac actgcggccc ggttgagttg atcaaccaga
     8341 aacccgtacc cgcgcgggaa agcttcggtc agcgaggcga tctcatccgc caagtcgacg
     8401 gccttctggt agacctgaag cttctcgaac atgaaagcca cgacgcgcct cggatttgaa
     8461 caggggaaca ggtgagcagg tgaacaggcg actgcagatt ctcctgctca cctgatcctg
     8521 cgctcccctg ctctattgcg gagcaggtga acagttgagc aggtgaccgc aggagagcag
     8581 taagtcttcc tggcaaaccg gagcagcacc cacgggcagc ccctctcccc tgctcacctg
     8641 atcctgtgct ctcctgctct attgcggcat acgccgcaat cggggcgact ggattcgaac
     8701 cagcgacctt ctgcacccca tgcagacgcg ctaaccagac tgcgccacgc cccggacgct
     8761 attttctccg ctcaaaccct aagccttttg aggctgcggg tcaagctttt cccctggcaa
     8821 cccccgcttt tcgccgcccc gctcacgctc tcgccatagc ttcagcgcca gaatcgccac
     8881 cccgcacacc agcagcgaat cggccacgtt gaaaacccac ggccagacct gtttccggta
     8941 aaacttgacc ggaatcttga tgaaatcccg cacatagccg acctcggcaa gctgggctgc
     9001 caccgccggc ggcacggcac gccccggatc gttcggcaga ggcgggtact cgcgcaatct
     9061 gaccgacccg tcgtccggat cggcaacctt ctcgtagtac cgcggcgcgc ccgcttcgga
     9121 caccccaacc agccgcacga acatccggtc gtacatgttg cccaaagccc ccgccagcac
     9181 ggcccccagc gcaagctgca ccagccagct tcgcgcagaa gtctgcgtga acatccacag
     9241 caccaaaacc agcgccaaca ccgacgccag caggaacagg cctgttcggc cgctgccgat
     9301 cccgaacaaa gccccgtcgt tcagcgccgt ctcaaaatgc agtacgtgcg ggatgatcac
     9361 ccgccgcccg ctttgccgca aagtcgtgaa cgcccagctc ttggaaccca ggtccagcgc
     9421 caggccgagg atggccacca accagaaccg gagatgcgca ggcagatgcc agatggcccg
     9481 caggcccctc tcccgctcag cgccgccacc accgccaccg attccggccg ggcgcacagc
     9541 gccgccgctc gtccgttcgc tcggcggtcc cgctaactcg ttgggtctgt tagccgacaa
     9601 aagctgatct aggcccgctt tccggtttct tgtgccagca cgtactcgta gctgtacttg
     9661 gcccacggct tggcccgaag gcgcgctttg ccgatcggct tgccggtggc ctggcagata
     9721 ccgtacgtgc cacgctcgat tcgttcaagc gcctcgtcga tctcctgcag gaccgcgcgg
     9781 ccgccttcga tcagccccag ggtgaactcc agctcgtagt tatcggtacc cagatcggcc
     9841 atgtgtatcg gcatgctcga tagatcgccg gccacctcct gccggctacc cttgagcgct
     9901 tcttttcgca aggtgtgaac gtcgcccaac agctcggcgc gcttctggag caacatttcc
     9961 cggaactgct ccaaatcctt cttggtcagg ccggcttcag cactggtccg aacctgcccc
    10021 gcaccctcat caggtgttga aacgcgctgg accactgcct tgggcgcggg ctgcacccgc
    10081 ccggccgggg cgcgcttctt ggcgctcgcc gctcttgtgg cacgcccgtt gccagccgga
    10141 gccttacggg cgcgtgcgct cccggcagag gaaccgccac gggacgtttt cttggaaacg
    10201 ttcttattga ccggtttctt cgccaccact caccgcctgg gccgcgtact gggcggctgt
    10261 aaaaagctcg acggacgctg attcctaggg catagcccaa ctagtatacg atcaccctcc
    10321 gtagcggtca ataagtttcg agagcagcag cgaccgaaat gttgcgtaag taactgttaa
    10381 ttcgacactt acgctcggat tgcggcctcc gagtagcgct ttgcagctca agccgggtga
    10441 tcgcttgccc gggcgtccgc ggcgcccctt attgctcgcc gcaggtcacc gacgctcccg
    10501 cactaggccg gaccaggatg agagcggcct gccggtcggt tgtgtcgtat cgttggtgca
    10561 gctcgcgccg gacgggatcg accgcgtctg cctgcgccaa ggccaccacg ctaccgccga
    10621 accccccgcc ggtcattctt gccccgagca cgccgggaac gcccgccagc agctcgatta
    10681 gcaaatccag ctccgcacac gaaacctcgt aatcgagctg caacgaaagg tgcgattcgt
    10741 acatcaagcg cccaaaatgt gccacatctc ctctttccag cgcttccgtc gccgaaagcg
    10801 tccgccgatt ctcggacgtg acgtggcgag cgcgagcggc cacttcgtcc ggcattcgct
    10861 ctgcctcggc cgcgacctgc gattcggtaa cgtctcgtag cgaccgtatg gccgggtcgg
    10921 cttgccgaaa gtgggtaacc gccgctgcac actgctcctg ccgcttggcg tattcgcttg
    10981 acgccagctc gtgcttcacg cccgagttaa ttaccaggaa ggtgtgcccg tccagccgca
    11041 gcggaacctg tcttatctgc aggtttcggc aatccagcag cagcgcgtga ttttcctggc
    11101 agaacagcga tgcgtattga tccattactc cgcacggtac accggcgaat tcgtgttccg
    11161 cggcccggca gatcggggct atctcctccc ggctcaacga cgctcccacc aaacttgcca
    11221 accccagcgc cgtagcgacc gacagggctg ccgaactgga caaaccggcc cccaggggca
    11281 agtcgctgtc aatcagtaga tcgaaacccg ccaccggcaa tccgcggcgg cgcagcattt
    11341 ccgcaacgcc cgctatatag ctggtccagt gcggcgcgcc ggccctggac cacttccccg
    11401 ccgcccaggt ctgcatgtcg tccagcgtgg ctgaacaggc ccgaatctcc ggatcgcgtt
    11461 cagccgctcg gcccgccgga ggcgctccga cggccaccca cgtcgcgtga ggcgtcgcga
    11521 tcggcagtac gaacccctcg ttatagtccg tgtgttcgcc gatgaggttg acccgccccg
    11581 gtgctcgtgc caccgcccgg cacgcgtatc cgaagcgcgc cgcaaacgcg gctcgcaatt
    11641 cgtcacgctg catgctgagc ctcacaccgc atgctggggt gactcgcgct cagctcgctc
    11701 gcgcagggcg ggcatcatac tcgtgatttc ctgcgcataa cggctgttgg ggtattcgcg
    11761 cacgatgcgt tcgcccacgc ttatcgcacg cgggaaattg cggtcggtga ccgccaggct
    11821 gaactcgacg cccatctgga acagccgggc cttgaagacg ccgcgggccg actccttgag
    11881 caccgccact tcgtccaccc ccaaatattt gtccagctca agtaacaact ggtgcgccag
    11941 gtcgacgttc tgcgccctga ccgcctcatc ataatccttg agcagtttgc gcttcacctg
    12001 ctgccgccgc agctccacct cgcgcgacag cgcctcgatc cgcggctcgt cggggaagag
    12061 ggtcttcagc cgcgttgtct cgctgcgcgc ctggtcccac ttgtgctgct cgcacagcag
    12121 atcgaaccgt ttcagggcgg ccgcgatctt ctcctcagtt gtggcgcgcc gcgcctgctc
    12181 gacctcctgg cggaaacggg cggcttcctc gcgcagcccc agttcctggt ccaggtcgcg
    12241 gatgaattgc tcggcgcctt cccaatcctg acgcacaata gcgcttttga tcgtgtcgtg
    12301 caggtattcg taatccttct cgcggtacac cacgcgtttt gcccaatccg acagcgaact
    12361 gttatctgcg attgtatggg tgtaatccgt ctgccgccgc aacaactcgg taatgtccag
    12421 cagcgcgccg taggtgcggt gcgtgtcgcc catcagacga tgtgccaggg ttagctgcgc
    12481 gtacatcaat atcgttacag ccagcagcat cagcccggcg gcaaccaggg ccaggcccca
    12541 caggtggtca ttctgtgtca gctcgaacaa accgagcccc atcgaagtca ggcacgccag
    12601 cgtgctgacg ataagtgcaa tcttgccgaa caggcgtacg ccccgaaacg gcggcggaat
    12661 gatggccatg tttcacctga ttgcggattt ggacggcctt ccgaggtcaa ccgcccgaaa
    12721 tgacgcctcc ttgcgcgccg gtcacgagag ccgcgtatcc agctacgccc agccgccgct
    12781 cagccggccg tcctcactgc ttcctcaagg cggctataca ccgccgcacg cgaagcgcgc
    12841 aacgctgccg cttcacttaa ttcggcactc gagcgtcaga tgattgagcc cgagtcgctg
    12901 tcgctgtccg tttcgtcggt gtcgtcgtcc gcgctttccg aaccgccgta ctcctgcggg
    12961 aagaacatcg gacgcatcca tgctttcaca tacgatacga acgcgtcgga ttgaaaaccg
    13021 aacgacacgg caccgaagaa cgcgtccgcc aaaccggtcg ccagccgagc catgaatttc
    13081 ggctgttgcg tcgtgccggt agtggttgtg tcggccaacg ctgtcggcgt ggttgcgctc
    13141 cggtcgtcaa tcttgggcaa tcggccaatc gccccgctgc cgccgcgcag ttccatagat
    13201 ctatttgcgg tcttgccgtt gtcggccccg gatttgcccg atgtggcgga cgtgccgctg
    13261 gtactgctgc cgccgctctc acccagaagc tgatcgcgca gcgccagcaa gtcctgtata
    13321 gtcatgccgc cggttcccgc aggtccggtt ccgccgctga acccgcccag caaatcgctg
    13381 attccgcccg caccgccgac caggtcgagc aggccgccga ggtctccgag cccgcccagg
    13441 ccgccggtcc cgaccgtgcc ggtcgcgggg ttgggttcca agccgtcacc ggtcccggcc
    13501 aattcgccct gccgcgggtt cgtaacccgt gcctcgataa aggcttggtg ccgttgccgg
    13561 gccgcagtca cccaagatgc gggtccgtgc gccgcggcct gcgccaagtt ttcggtgggc
    13621 gtctgcgatt gcgggtcagt cgtcgtagta gtcgtctgcc cgagagccgc aaccgccagg
    13681 aagcaaacaa gcgtgacagc aaatgagcgg atcattagca tacctctatc gctcacgata
    13741 gcttcaatcc acaaccgaac ctacgtatta taccgtaatc ggcaaatgtc ggtgacgcaa
    13801 cagcgggccg attcgacaga gagctacatg gccggcgtta tccgaacctc cagtttcacg
    13861 gcgacggtac tcgtattaac gtttgcctcg ctttttatcg ggtgtggaag agatccggag
    13921 cgcgccgctg cgggccggac gctcgccgcc aatactgtga tcggatttat cgggcctccg
    13981 gctaccgacg ccatttccgc cgctgtatgc ggtggcgccg aacgcttcgc ggcggcttac
    14041 ccgaatgtcc actgcctcac ggtcatcccg cccgacgccc agccgtcatc tctatctgcg
    14101 gccgtcgacg agacccttgc gcgaaaccca aacgccattt gcctttatct cacgagcacg
    14161 cgtgctccaa acgacgcaga tcacaccgcg cagcttatcg aacgcatccg gcagacgggc
    14221 atcgtgctcg taacggttgg gtcgcccatt aacaaccccg ccgtccgcgg ccacgtcgag
    14281 gtcgctctgc cgcaggccgc cgaaatcctg gcgcagaact tgcggtcgct gttgccgccg
    14341 gccgtgaacg ctttcggcca tgtacggcag gaacgcagct acgcgctcct tcacgcggac
    14401 gggcgcgatc agtccgcctc cgatacgtac gcgcgctttc tggcgaccgt gcgtgacaac
    14461 tcaaacctgg cccgcctggc ggacgtcaat acctgggcgt cggatcgcag cgcgcagcgg
    14521 ctgatcgccg atacgctgga gcgcttcagc accgttgagc tgatcgttac cttggacccg
    14581 gagccatggt tatcgcttcg gcctcgcttg cggctgcccc cacgcaatca tttcgcgact
    14641 ttaagcgccg ccccgcgcct ttggccgcga cttgagtccg gcgaggcgct cgcgctggtt
    14701 ggcccattgg acgggcagat cgggtacacc gcgatggaag tggccacgca acggctgatg
    14761 ctcgttccgg atgctcccac gcgtcgctac attccttgtg agttggtcac gaaggccaac
    14821 cttgacgact tcgcccagcg ctacgccgcc gccgcgaacc tggacgtgcg cgacctgctg
    14881 tcgttccggc cactgcccta accggaagcc cgcgatccgc aattctcgtg gtgaacagcg
    14941 gttggcctcc gcggctataa catgtgcccc gatgctgctg cgtgtcttgg tggctcgttg
    15001 gccctccgct acaattccaa gctcctgtcc gatcgcgagg tgctgcatgg catgggtctt
    15061 gatgacgctg ctgctggtcc tgagctgggg aattttcgcg tattccgcgg tgcggcgcgt
    15121 gcgactgatg atggtcgccc aggcgcccga caaccggtgt gatcatctcg gcgagcggat
    15181 caagtcggta ctggtctaca tgttcggcca gaagcgcatg ccacgctatc cactggcggg
    15241 gtgggcacat atcgccgtgt ttttcggctt catggtgcta ctgctgaact ccctcattct
    15301 ctgggcacgt ccgtactttg gaccgcactt cgcctttggc ttgttcggac tgcatcagcc
    15361 attgggcgtg gcgtatgcgt tcttgcgcga cattttcacg gtgttggtca tcatcggcgt
    15421 gctgatcttc tactggaatc gcctggtcgt tcgcctgggc cgcctgacgc taaacctcga
    15481 aggcgtcctg atccttacca tcatctttgt gatgatggtc gcggacctgg tctacgaggg
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggccgcgtgc cgccgatcca
    15661 ggacatcgag ggccggctgg agcgcgagga aacgctcggc gccaagcgca tccgggattt
    15721 cagttggaaa gccgcgctgg acttctacac gtgcaccgag tgcgggcggt gcagcgacca
    15781 gtgccccgcc acgcgaaccg gcaaactgct gtcgcccaaa catttgtgca tcgcactgcg
    15841 cgatcacatg tacctgcgcg aggagcagtt gatcgggccg cgcttttcgc tgaagcgcaa
    15901 gccggaagac caatcaaaga ccgattccac agagcagggc gcggtggaag tggaagcgcc
    15961 cggcaagccg catggtacgc gcgaccacca acgtctgctg cacgatcccg ccgcgccggc
    16021 cgtgctgcac accgagcaac tgacgccggc gtgggttaat ccggaggtgc tgtgggcgtg
    16081 cacgacctgc ggggcgtgcg agcaggaatg cccggtcttc atcacgtaca tcgacaagat
    16141 cgtggacctg cgccgcaacc tggtcatgga gcgcggcgaa ttccccgagc agttgcaggc
    16201 cacgttcaag gggctggaga cggtcggcaa cccctatagc ttctcgcctg agcaacgcgc
    16261 ggagtgggcc caggggctaa atatccccct gcgggccgaa aagcccgacg cggagtggct
    16321 gttctgggtc ggctgcgccc cgtccttcga tgaccgctcg cgcaagatcg cccgcgcaac
    16381 cgcggaactg ctgcaggaag ccggcgttga tttcgccatc ctcggcccgg aggagcagtg
    16441 caacggcgac ccggcccgcc gcgcggggaa cgagtacctc ttccagatgc ttgcacaagc
    16501 caacatcgag acgctgaacc gctacaacgt caagaagatc gtcacgactt gtccccactg
    16561 ctatcacacg ctgaagaacg aataccctga tttcggcggg cattacgaag taactcacca
    16621 cgccgagctg ttggcccaac tcattcagca gggcaagctg cggccgcggt tcccgctgaa
    16681 ggcgacgatt gcataccacg acgcgtgtta cctcggccgg cacaaccaga tttacgatgc
    16741 gccgcgcgag atcatcaacc agattcccgg cgcgtatgtg gccgaagcgc cggaaagccg
    16801 cgatcgcggc atgtgctgcg gagcgggcgg ggcgcagatg tggaaggaag aggagcacgg
    16861 cgacgcgcgc gtcaaccaca cgcgcgtcaa gcaactgctg cgcgtgattc ccggcggtgg
    16921 cacaagtaca acgcctgcgg gcacgaagcc gaccattgcg accgcatgcc cattctgcat
    16981 gactatgctg accgatggct gccgcgatca ggggtttgat gacgtgcagc agctcgacgt
    17041 ggcggaaatc ctgcaccgcg cggtaaaggg gccgaaggaa actcagaacg ctgccgaagc
    17101 gtccgcggcc cagccggccc gatgattcga aaagtcaatc ctggtccgcg cccgcggcga
    17161 accctacatg agggccggtg tgtcagccgt cagtcaacaa gagcatgaac gcatcgatgt
    17221 cgaacgatcg gatctgtcga tttccgcatg cccccttctg cgctcgctgg ggtgtgttaa
    17281 gctctgcaat tgacagcgcg gccgaccttg ggctgccgtc ggccgcgcta gtatgttcaa
    17341 gaggttctta tgtggttccg taagtggatg attcctatga tgatgtccgc cgccggcctg
    17401 ccgttcgcgc acgccgatca gaccccgtta attccccgcc agacgctgtt tggaaatccg
    17461 cagcgtgctt cagtgcagat cagccccaac ggtcagtacc tggcgtattt ggcgccggtc
    17521 gacggcgtgc tgaacgtctg ggtcggccca ctcggcgacc cggccgccgc caagccgatc
    17581 actcaggaca aaggccgcgg catccgcatc tacttctggg cattcgacgg gcggcacgtg
    17641 ctgtacctcc aggacaagga cggagacgag aactggcgcg tgtacagcgt cgacgtcgac
    17701 cgcggggaca tccgcgattt gacgccactg gagaacgtcc aggcgcgaat ccaaaatctc
    17761 agccaccgca agccgaacga aattctgatc gccctgaacg atcgcaatcc cgagcttcac
    17821 gatatctacc gcctggatct gacgaccggc gagcggcaac tcgtcctcga aaatgagggc
    17881 tttatggcct tccacgccga cgaggactac cagattcgcc ttgccgaacg caccactccc
    17941 gacggcggac gcgaaatctt ccaacgctcc actgacggga catggcagcc gttcatccag
    18001 cttgcgcccg aagacgccct gagcacgaac gtggccggct ttgataagac cgggcgcgtt
    18061 ttgcggctca tcgatagccg cggccggaac acggcggcct tcaccgaaat ccggctcgac
    18121 gacggcaaac aaacgctcat tgcagaggat ccgcgcgccg acgtcagcgg cgctatggcg
    18181 caccccaccg agtttacgct cgaggcggtg tcattcacgt acgagcgcgt acattggaaa
    18241 gtcctcgatc cgaagatgga acctgatttc gccgcgctgc gggccgtggc cgacggcgat
    18301 ttcgaaatca ccagccgcac gctcgacgac acaaagtgga tcgttgcata cgtgctcgac
    18361 aacggcccgg tgaggtacta cctctacgaa cggccggccc gcaaagctaa gttcctgttc
    18421 acgaatcgcc cggaattgga gaagctgact ctggctcgca tgcacccggt catcatcaag
    18481 tcgcgcgatg gcctggacct cgtcagctat ctgtccctgc cgcccgagtc tgatccggac
    18541 aatgacggcc ggcccgaccg cccgctgccg atggtactgg acgtccacgg cggaccgtgg
    18601 gcgcgcgatg agtggggcta tgacgcgtac caccagtggc tggcgaaccg cggatacgcc
    18661 gtgctgtcgg tcaatttccg cggctccacc ggcttcggga aagagttcac gaacgccggc
    18721 gaccgccagt gggccgccaa gatgcaggac gacctcgttg atgcggtaca gtgggcgatc
    18781 cgggagcgcg ttgccgaccc gcagaaggtc gcgatcctcg gcggcagcta cggcggctac
    18841 gcggtcctgg tgggcctgac gttcacgccg gagttgtttg catgcggtgt ggatatcgtc
    18901 gggccatcca gcttggtaac gctgctcgaa tccattccgc cctactggaa gccgattctg
    18961 gacctgttcg cggcacgcgt cggcgatccg cgaaccgagg agggccgcaa attcctgctg
    19021 gaacgctcgc ccataacgcg cgtagaccag attcggcggc cgctccttat cgggcagggt
    19081 gccaacgatc cgcgcgtcaa gcaggctgag tcggaccaga tcgtggccgc catgcaggag
    19141 aaaaacatcc cggtcactta cgtcctgttc gccgatgaag ggcacggctt cgcgcggccg
    19201 gagaacaaca tggctttcaa cgcggtcacc gaggcgttcc tggcccggca gctcggcgga
    19261 cgttttgagc cgatcggcaa cgacttccag ggttcgacta tccaagtgcc ggtcggggcg
    19321 gaccaagtgc cgggtttggc cgaggcgttg aaacagcagt gatgcaaggc nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atggagccac gcatgaaacg caccctttgg
    19501 gggttaccgg ttttcctcgc cggcgccgta atggtgctgg ccgctcaaca accgcccgcc
    19561 actccggccg agccggacga cgggctggat gccttgcgcg ccaaagattt gctgggtgat
    19621 gacgatcgcg ccgcgcttcg cgcctggatc ggcgaacgca ttgcgatggt gatcggcgat
    19681 gacgtcgctg gcgcccagca ggccgtcgct cagttgcgtc agggcgcacg cggcggacgc
    19741 ggcttcacgg atgcgtacgt tgccgcgacg atggagagca tcggcagcgc ctacaagaag
    19801 gctgagctgg tgccggcgtc gcggctgctc atcgtgctgg gcgagctgaa cgacccgcgg
    19861 accttcaata ctctgctggg cgcactgcag gatgagcgta ccggggttcg cgcggcggcg
    19921 gccgtcgggc tgcggaagtt gcagtccaag ctggacccgg ccgcgatgaa cgaaatgctg
    19981 gcggccctgc gcgcggcggg cgaaaaggag accgcgccac ggacgctcga tttgatatat
    20041 cgcgccatga acctgacgga ggcgcccggg ctgacggaaa gacgcgccag ctcgaccgcg
    20101 atcgtggatt tgttgcagca gcgcttgaag agcatcgaga acggtcgcat gcgcgccgaa
    20161 ggcggcgatc tggcggcctt ggaagcgctg gatccgcagc gcggcaactt ggacgacgcc
    20221 acccggcggc gatacatcgc cgagttgggc cggctaatgc actattgcgt gtgccgctac
    20281 gtgcgcgatg atcccctgtc gcgcgtccgc gatcagagca gcagtccgga cgtggttgaa
    20341 ctgcgcaaca acatcgaagt caccattcgc gaggctgagc ggcaactgca aaatctggtc
    20401 ggtgctcccg aaggcgcgaa catcgccgag aacatgaccc gcgccaaaat cacggagatg
    20461 gccatcgcca tgaacaagtg gtcggagaaa ctcaagggcg tcgacctcga cttgccggag
    20521 gtgcagcgcg ctccgggcga tgagcctggg gggctatagc acctggccca cccgcgcaca
    20581 gccgcctacg accggcgata atactgggcg ttgcgctcga taaaatcgcg ccatttctcg
    20641 gggacttcat cctcggggaa aatggctttg acagggcatt cgtccacgca caggccgcaa
    20701 tcgatgcacg tatccgggtc aatgtaaagc tgctcagcgg cctcgaaatc cggctcgtct
    20761 ttgcgcgggt gaatgcaatc gacggggcat acctcgacgc aagctgtgtc cttcacgccg
    20821 atacaaggct gcgtaatgta gtgcgacacg cgccaagctc ctggcgctgc acgcccgacc
    20881 ggtcgttgca gcgccgaaat actttacttg aactcgacca ttatgccgtt cgcggcgaca
    20941 ctgagcgatt ataccaaaca gcgggtgcgt tgcctgcggc agtggcttta tcaagcaacg
    21001 tttcgccctc tccgatgatt ttcgtcagca atatgccccc acccagccgt gccgccgacg
    21061 tcacgaccca gaatgcggcg ccggtcgtcc ctgctgcaaa taatggtctt ccgcctccag
    21121 agccatggat acccgcttac gcaaaaccgc gctcagtggg cggctggctg agctgggcga
    21181 ccattggctg cgcgggcaga tcagatccct cagccggctc gtcaacgctg ccaagccccg
    21241 tggcggcgcg atcatgcccc gcagggcctc catatccgcg cccaactcgc ccgccgtcgc
    21301 gtagcgctgc gcctcatcgt gtgccagcgc gttgcacaga acggccttta gaaagtagtg
    21361 aaattcgccc agattcgacg gctcccgccc gcgcttatcg gttggacaca cgcgcgaaac
    21421 gtcaaggtgc aaccgcccct ggtccagccg cggccgccgc ccggtcagca actcgtgagc
    21481 aatcaagccc aggctgtata catccgagcg cggcgtcaat tgctccgtga gaccccgcgt
    21541 ttgctccggg ctggcatatt cgggtgtgcc cacaaaccgt ccgcgctcgt tccgcagcgc
    21601 gtcagcctgg agctcggcca gccgggcaat tccaaaatcc agcactttcg gctgcccggt
    21661 cgtatccacc aggatgttgc tcggcttcag atcccggtga atcacgccgt gccagtgcgc
    21721 gtactcgacc gcttcgcata cccggcaaaa aagtcccagc cgagcatcca cgtccaactt
    21781 atgcgattcg gcgtatctga tgagcgactc accctcgatg tattccaata cgaaatacgg
    21841 ctggggcccg ctggtggtgc tgatttcgct ggcatgagtg atctgggcga tgcccgggtg
    21901 tcgcaattgc cgcaggatgt gagcctcaaa cgcgaatcgc cagcggtcct cgtaagagac
    21961 aaggtccttg cgcaacacct tgatcgcgaa ttccttgcgt agcgtggggt agacggcccg
    22021 gtacacgacg cccattccgc cgcgccccag tacttgcacg acgcggtact gccccagggc
    22081 gaacggcagc tcgggctcca attccggcgg ctcctgctcc accgccacgg caatctgccc
    22141 gtatgttgtc gtgcccggca tgccgagggc cgtcttctgc ggccgctcca actcaaacgc
    22201 gcgcatctgc tcgtaatgcg gcagcagcga gagtacttcc tgcaaaagca ggtcattgcg
    22261 gccgcactcg cgctttagat aggcctgccg ttgcgcggaa tccaaactca aggcccgctc
    22321 gagcagctct tgcacgcgca gatgaaactc atcggacatc ttcggagtta cccgtcaggc
    22381 gacgattcag ccacgccagc gcgtacctga agtccttttc gacagtgctg gcgcttacgc
    22441 ccaggcagcg cgccacctcc gaatatttca gccccccgaa ccagtgcagc atcaccacgt
    22501 ccttaagtcg cgcgctctcc gccgccaacg catccagcgc gtccgccaga tcgagcacgt
    22561 ccactgtccg gtcgcgccac tcgacgctga tcccctccag cggcacgcgc tgccatggcg
    22621 tgtcatcggc ggccccgggc tcctccgccg cctcctcaga tgcggtagcg gacgccaatg
    22681 gcactccgcc gcgttttctg gcatagcgct tcttcagatg atcgacgata acttgccaga
    22741 ttttccgggt cgcaatcgcg cgaaaatgct ctgaactctc ccactcgatt cgcgaatgct
    22801 gaatcaggtg caaacacgcc tcgtccacga cctccgtcgg gcgcagcgtg aaacccgggc
    22861 gctgattgcg aaaatagcga cgcgccacct gccgcagttc ctggtaaacg agcggaacnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ttgcgaaaat agcgacgcgc
    23041 cacctgccgc agttcctggt aaacgagcgg aacaagctcc tcagtcgcgg cccgtcgttt
    23101 tttttcaggt gccggcatgg cggtttccgg tttcggacta ctcggactac cagagtcgag
    23161 cccagacggc ccccagcgcg tgcttcattc taaggaattg gcccgacgcg cgtcaaaaag
    23221 gctctgccgc gcgcccaaga tgcgtcgccg acgccgccgg cccctccgat agctcaaaat
    23281 ccgcgatgac cggcaagtgg tcgctcgcca cccgcgccag cttcaggtgc gatcgccgta
    23341 cctggacggg gcgcaatggg ccgcggtaga agattttgtc cagcccgccc gtcggggcga
    23401 tgctcgggaa ggtcttgatc gcccagcgtg accccggccg ccggttggtc gcgcagcaga
    23461 agccggccgg cacgaagaaa cgccgcttga gcatgccctg ccagtcattc atatcgccgg
    23521 ccaccacgca gggcgtgctg gctgaatcga acgccgcgat aaacgccagt aaccgccgca
    23581 tctggtggtg ccgtagcagc gccatcaggc ttaagtgaat gttgaacacg tcgatttcgg
    23641 cctgctgtcc gttgcgcacg acgtgtatgc ggctgtgttg cgccccgcgc cgcttccgcc
    23701 cgccgatcgt caggtccagg ttgtgctgcc gcccgatggg gtagcgcgaa agcgtcgcgt
    23761 tgccgtattt gccgcagcgc atgaacacgt tcatgccaac ggcgcggtaa gcgtacccca
    23821 actcgcgccc caggaacgcc gccaggtcca gcatcccgga gcgcggcact ccgcggtcca
    23881 cctcctgcag cagcaggacg tcggcctggt agtggcccag gacctccacg atgcgctgcg
    23941 gcgcgaactt tccgtctatc ccgatcgctt tgtgaatgtt gtacgtgagc agccgcaggt
    24001 gcataaacag attgccaagt ataacgcacc cacgccccgg ccgccacgtt tttcggcgac
    24061 ggagcgggta ggaaacgtgt tccgcagcgg aggcgcaact agcgacagtc acgtgactgt
    24121 ccctggttat cgggttaaga ggcgcaggag aagagcatga tgattaagcc gccgtcctcg
    24181 cccggagggc gatcgactgt tgctaggcgt ttcaattatc tggcgcctgg caaagccacg
    24241 ccttaacttc tcttcccctc ctcttccgcg cccggaggcg agcgattgtt gccaggcgtt
    24301 tcaacgtctg aaacccggcc cacgcccgcc gagtccccca cccgcctttc ccgttcacac
    24361 tttctcacgt tctcaccctt cgcggccgat acgccaggac taaggcataa caaatatcat
    24421 ccctctccgc gcagcggccc ttgttacctg ttcacttctc acctatcacc tctatccgaa
    24481 gtgcggggcc cccctatggc tgcggggggg gcgggaaagg agtcccgccg cagcgcgtgc
    24541 gagaccgatt ctcgcactag cggccgcggc tgcggtcttc ggtccagacg attgagagtc
    24601 ggacgtagag gggagcggag gacgaataag acgagtgaga gggatgggcc gcaatggcat
    24661 tggcggtcgc agccccgctg ggcgctgccg ctcgtttgcg gccttgcggc gggtttgcga
    24721 tgatggtgtg aacgacccat ttggccgtgg atggagaaga ccatggatcg caggacattc
    24781 ctgaagaccg tgccggtgct gacggtggtt gccgccggtg gtcgcagttt cgcacccaca
    24841 attcgaggga atgaaggacc cgcaacggca gcaggcgttc tacaacgtcg cggccggtct
    24901 g
//
LOCUS       DAOSYS010000019        23943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1741_length_23943_cov_5.022605, whole genome shotgun sequence.
ACCESSION   DAOSYS010000019 DAOSYS010000000
VERSION     DAOSYS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23943
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_23943_cov_5.022605"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..4112
                     /locus_tag="PLU99_02225"
     CDS             330..4112
                     /locus_tag="PLU99_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15900.1"
                     /translation="MNFTKPNSPIFSLVATTILLCITLLSGCPEPQVPREASENSNGV
                     PPANAFIPAEEIIRSPTPVQLETSGKSDEEEPWHGGYGDGGSGNGSGGGSGGWGGTTR
                     GMAHISFTAPLTPLAVKPGGKVAVRFSVTLDPDAYIAAADLLIARDRDADRKPDGDPV
                     YVQDITELVAPGDNAFLLLTQVLADLGLLQNGFGRFLVGIAVHTNAGHTTEAYASGTI
                     TIDAVRPTMSGFRAGRDGLHADQEDHLIVNTTAVWVIAMETYDNGPHRIKVLLDPDKV
                     PLSGNEVEVLDQLVNPGSQQFVIHPRDIAPNNYFYYFTIFDEVAPSFAGYAANSSTGA
                     HFRLGITARLVGDFNFNWLDPDIPGEYRNGTGPSMGAILQGFNFNDLAGSSMCSVPDV
                     NGDALPELLVAARFGKPYVESAQGIGFGEAYLFYGQADRLRDKFQLNSAGLESVDYPT
                     LVFPGIRAPKNETWTYGLADIAAIPDMDGDGYPELVFCFPRVESVPLILGRNEAEMKV
                     QSVPPDGVVGDANLEYNAYRYDEDEGKFRWFPNEAQFTRGGIVIVSSHSYLLRQTDHL
                     NRRGDRVLDLAEVGQIFNSQGAPAYVPFIWGAVNEPDTAPCGVPPVPTPYDDWTVYWD
                     IVLFNQGPGGFDNAYTNAAFARPQAAPATGFEPDPFQPPLANARTLLKLDVLNGVVPP
                     VDDKCGTQCVVANAWRTFGNCSLPSGGGFPGAWDGVCETQSWHGSFNKSESGQAIWTG
                     FYGPNNPGVAINGDTVGARILGQRREDRFGSSVAADGTYLYISSPECVAGSRNRAGAV
                     YQMRTFARPGGSATTQTQLWIEPRDPAGTFTPIWPNVDVEDAARRDWTMPVPHQYIIR
                     TGGSVRGADVYTLDGAFWPPPFGEPVGSSDRLVNYVFQGAGCLQSVAWSVNGGLAADT
                     LLPAPGGSPFWPAWGNGTAAYYVETTRQYVGPHVGAKISQVRIVGDLNRDGIPDFGIG
                     SPDVRTSFGDPEHPDGCKAGAVFIVLERPTGVGGEVDLGRIALHPDHPRRLAGVMIRG
                     MCGGQVFGRVFDAAGDFNGDGIDDVIIGSEGYDYNRGQAVILLGSPTLASPQGGWTID
                     ELISVGRGIRFRGAAVGDLAGTNVAGIGDMDGDGIDDVLIAAPGALNGRGAVYLIYGS
                     PALTGQDVSLGDIGKIDLPGARFIGRAVGDGLGGGRLVFDGDNPQADRRMYLNPGDPT
                     EPYYRTVEVYSRGVAKIGDIDGDGNADFAISSMLADPGGNVDAGEVYVIYGKSGEE"
     gene            complement(4192..4614)
                     /locus_tag="PLU99_02230"
     CDS             complement(4192..4614)
                     /locus_tag="PLU99_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOO15901.1"
                     /translation="MSKKTVQQRNTSQRDTIAQVIRAARGPLTIAQIHERAQRRSRGL
                     GVATVYRTVKLLLTTQQIHVLRMPDGEKRYESAHLDHHHHFRCRKCEQVYDLPGCPLA
                     IADGTKLPAGFEVDAHEVTLFGTCPSCRPGKRSEAQRG"
     gene            complement(4665..6104)
                     /locus_tag="PLU99_02235"
     CDS             complement(4665..6104)
                     /locus_tag="PLU99_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOO15902.1"
                     /translation="MNVRLTLYVLGGLLIFLGAALLLPIPFSIWYHDGQLLTFVLSAA
                     ITSLVGGFLFWRFRREREEITLREGFAVVTFAWIGFALFGSLPYIISASLTNPVNAFF
                     ESMSGFTTAGASVFTDVEAVPKSVLFWRALTQWMGGMGFIVLGVAILPLLGVGGMQLY
                     EAEAPGPTAERLTPRIQDTARLLWGVYALLTGLGLALFWLGEMDFFDALCHTFTAVAT
                     GGFSTRNASFAAFSSYSQMVALVLMLLGGVNFSLHYYALKGRARNYWSSSEFRFYLGV
                     LGGATLVVFLANLALSGNWLANLRNSAFTVTSILTTSGFTTADYEAWPFLCQAVLVTL
                     MFAGGCAGSTSGGVKQVRLLLLFKHALLQLRRLVHPRQVRILKLDHRPVPQDVMQDIL
                     GFVILFVGFLVVATLLLTAVGIDLITAGSAVIACMSNVGPALGEVGPSNNYAPLPAFA
                     KVVLSFAMLLGRLEVSTVLVIFFGSLWRK"
     gene            complement(6101..7444)
                     /gene="trkA"
                     /locus_tag="PLU99_02240"
     CDS             complement(6101..7444)
                     /gene="trkA"
                     /locus_tag="PLU99_02240"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOO15903.1"
                     /translation="MYVIVVGAGEVGFHIADRLSKEGHDVTVVERDVEKGPILRAKLN
                     ASIVLGSGATAEVLEQAGIDRADLFIAVTDQDEVNLVACLLAHERNTRRIIARIKGVE
                     YTGAGWTERIGKLGIDLLINPQKVVAEEIHRIVSYTTAAEVAEFAGGRVVFLGYAIGR
                     NSSLAGISLRDLAGIRGLYRMVVTAIARKHRTIIPRGEDTIEEGDIVYFVCHKPDVPA
                     IDYLFEFEKHPSKTVFILGGGGVGAALARKLAGLGIRVKLIEHNARLCERLAEELDNV
                     AVLNAEGMDIDTLKHEGIEGCDAYVAVTQDEQTNILCSLLAKSYGAKRAIAIVDRHEF
                     VTLAPSLGVDACVSPRLVTASAILKYVRPPGVTSLATLEHGNAEVLEIVMPTASPVLG
                     RPLKDIDVPEGAVIGIIVRGSEVVIPSGEDHLEAGDHVIVFALPEAIPRIERFLK"
     gene            complement(7718..>8466)
                     /locus_tag="PLU99_02245"
     CDS             complement(7718..>8466)
                     /locus_tag="PLU99_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928126.1"
                     /note="in Escherichia coli this enzyme functions in
                     thiamine biosynthesis along with thiFSGI and IscS; with
                     ThiFSG catalyzes the formation of thiazole phosphate from
                     tyrosine, cysteine and 1-deoxy-D-xylulose-5-phosphate;
                     forms a complex with ThiG; contains an iron-sulfur center;
                     in Thermotoga this enzyme has an extra C-terminal domain;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOO15904.1"
                     /translation="ALSRAQEAGIDDVGIGVLFGLYDWRFEVLGLVAHALHLQQRYGV
                     GPHTISFPRLRPAQGVMHQDQYLVSDQDFKRLIAILRLAVPYTGLILTAREEPALRRE
                     VLGFGVSQIDAGSRIEVGGYTEAGDAQVMEREQFELGDIRPLDTVMRELLEDGYVPSF
                     CTSCYRLGRTGEHFMEFAIPGFIKRFCTPNALTTLLEYLVDYATPQTRAAGEKLIAAE
                     LEKMEEGPLKRELLDRLQRIRETDARDLYF"
     assembly_gap    8467..8566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8567..9300)
                     /locus_tag="PLU99_02250"
     CDS             complement(<8567..9300)
                     /locus_tag="PLU99_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO15905.1"
                     /translation="MTMRLPEMTLSDGATDFIRDAELETVLACAQPEPARVREIIAKS
                     LSKQALSLAETATLLKADDPDLTEEIFAAARQLKRDVYGNRIVLFAPLYIGNLCSNDC
                     QYCAFRRSNPDAVRRTLSPDEIRAQVAAMQNRGHKRLIVVFGEHARYNAELIADTVRL
                     IYTVKVGHGEIRRVNVNAAPFDIEGFRTIKAAHIGTFQIFQETYHHETYRRYHPLNTR
                     KGDYLWRLDALSRAQEAGIDDVGIGVL"
     gene            complement(9329..10471)
                     /gene="hydE"
                     /locus_tag="PLU99_02255"
     CDS             complement(9329..10471)
                     /gene="hydE"
                     /locus_tag="PLU99_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOO15906.1"
                     /translation="MNRVQIINWLLENDSARLEKLWHDADSVRRTHVGDAVHLRGLIE
                     FSNHCARQCYYCGLRADNRLIERYRMPADEILACARQAASFGYGTVVLQSGEDYGITS
                     EWLADVVRHIKTETGLAVTLSVGERSAAELAAWREAGADRYLLRFETSNAELFRRIHP
                     SRNGQLSDRIAILRTLRQLNYEVGSGVMIGIPGQTYEDLARDLELFRELDLDMIGVGP
                     FLPHPETPLGQSFDPLIPGSLDPSPSGQVPNSELMTYKVLALTRLMCPRANLPSTTAL
                     ATLNRDSGRELGLMRGANIIMPNLTPPAYRCKYEIYPAKACLFETAEQCHGCILRRIT
                     RLGRTIGTGHGGSPNWLARRGNDQGSRESGGQEGAARDSCLEFLDP"
     gene            complement(10449..11609)
                     /locus_tag="PLU99_02260"
     CDS             complement(10449..11609)
                     /locus_tag="PLU99_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO15907.1"
                     /translation="MDTLRVLIVDDEPGMQYSVARALRHFTVQLPDVEGEVCFETETA
                     SSGEEGLQKLASWSPDILLLDYKLGDMTGLDVLEHLVKHEREVLTVMITAFATLETAI
                     RATKCGAFDFLAKPFTPDELKETIRKASQHLVIQRQARKLAAEKRRVRFEFISVLGHE
                     LKAPLAGIEGYLHLLKERTAGNDLSAYDHIIERCLERAGGMRKLILDLLDMTRVESGE
                     KKRELVSLDVREIAKGALDTVRPDAEKHNLSLQLHGDGPVLMVADRNEIEIIFNNLLS
                     NAVKYNRAGGRVDVHLSGDEQRVTIAVSDTGIGLTPDDAGRLFRDFVRIKNDQTRHIT
                     GSGLGLSLVKKLALMYGGNATVTSKPGAGSTFTVTLQKNARPPGEHEPRADN"
     gene            complement(11611..13629)
                     /locus_tag="PLU99_02265"
     CDS             complement(11611..13629)
                     /locus_tag="PLU99_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOO15908.1"
                     /translation="MDPPNAPLVTTIRERCRRCYTCVRECPAKAIRIASGQAEVLVDR
                     CIGCGNCVRMCSQGAKQVQNGIQAAEELLKSGARVTAIIAPSFPSEFTEIDYEFFVAM
                     VRALGFHLVHEVGFGADLVAAEYQRLLRLYPRQRFISTTCPAIVGYVERYHPNLVPSL
                     APIVSPMIATARALRRLYGKNLKVVFIGPCIAKKGEAKSERVAGEVDATLTFIELREM
                     FLHHAIKAESVTPSDFDPPHAGTGSLFPISRGMLQAAQIPEDLLSGAVVAADGRTEFI
                     EAIKEFERGDLDARLLEVVACNGCIMGPGSSTRAPLFSRRSQVSRYAKRRMSDADREY
                     WHEHWSALCELNLRRTFSPHDQRIEMPSEEQLTPVMERMGKFAPEDELNCGACGYETC
                     REHAVAIVKGLAESEMCLPYTIEQLKNTCKELAYSNEQLASTQEALMQSEKLASMGQL
                     AAGIAHEVNNPLGTVLMLSHLLLEEAAVDSEMREDLNLIVSEADRCKKIVSGLLQFAR
                     KNKVDFESVDVREIVARCTRALHVPENIEVHTAHDGDYTEAELDRDQIAQVLTNLASN
                     AIAAMPQGGKLSILTNGDADKVRFIVSDTGIGIPPTNRKKIFEPFFTTKPAGEGTGLG
                     LAVSYGIVKMHHGDIKVESNADAAAGPTGTTFTVTLPRRRLIQAVNGK"
     gene            complement(13755..14210)
                     /locus_tag="PLU99_02270"
     CDS             complement(13755..14210)
                     /locus_tag="PLU99_02270"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOO15909.1"
                     /translation="MAHIRPRNRKREHPRAALNAAPIIIDGTAAAERPVTCELAPSVS
                     LRDSLAVQRNALANERTFLAYARTALVFAVAGMALVQFFGPLALQLLGGLFICLGVLT
                     LALGGVRFMRVRRRIRQFAGAGRRNDRKEASAAENGANGRRGPEACNRA"
     assembly_gap    14517..14616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14730..15566
                     /locus_tag="PLU99_02275"
     CDS             14730..15566
                     /locus_tag="PLU99_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO15910.1"
                     /translation="MLWSRSTKLAPSPVDLDDRVVAELHCIKCGTPLRGEFASGSCPQ
                     CGHPCSDSVYGDYLIYSDRAEVDRLDEAARVVIYGGMLLGVIIAIAMLFMVTSSQNVI
                     EAIRRGFDTLWAGVLLSPVIATTGMVLLTRRYSMAYYEAKYFHRRFLIRAGICAGLVL
                     ILAGVAAYFRARDFQVLLLAGWATLPSAAFMRGLARLMRRVPNVKLASFAQALSAIIW
                     VNGVLSLLFNWLRPLGAESADWEAPLLSLQMLVSCGWIGLGIASLRLMMTVHRNFLAI
                     RR"
     gene            complement(15602..18100)
                     /locus_tag="PLU99_02280"
     CDS             complement(15602..18100)
                     /locus_tag="PLU99_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15911.1"
                     /translation="MPKLARTLVNKPAATTRPRAAARVAELLNMRLTARQHLWLLVVL
                     LVALAVRGAYFWGQYRNSPLFDFPQMDGRVHHEWAASIASGEGMEPRPYLRAPLYYYL
                     LAWLYMLTGPQVAVARIAGCLLGALNCYLMARLAAALAGFRTGLLAGLIAALYWPLVY
                     FDFELLTVGLEVCLNTALLWALLAAGRRGSTLWFAAAGVVWGLSAITRPNVLAVAPVI
                     WLWAWWTSRQPSAARRLLAPLAATIGLVIPIAPVALRNYFVGGEPVLIASTGGINFYI
                     GNNPESTGYLAIVPEARPEWRDWLVDIKQIPEQELGRSLTDREISSYWFGRAMKWIGS
                     APGAWIGHMVTKLRLFWSPLEIPNNQPIWQIARQAGWIAWLFWIGFPVVAALAIPGLA
                     LLRGRWRAWTIPVAFAAVYMATVVAFFCPARYRAPVIPVLIILAAFAVCSVAEYIRQR
                     RWTPVALFAAGVAVVAGLIATNPPGSATYRQRVEGEYHFILGRHFATPAPYGGQDLER
                     AEQEYRQAIELAPYIPQQHTALARTLFSLGRPQEARQEFLNALKAGPRDGLTHMYFGL
                     FLASMREMELAISAYQTALHFQPSLALAHQHLGAALMQTNQDDAAAQHLRKALEIRPE
                     LTEARLNLAAIMLRQAKSDEEVQAAVTEFEAVLREKPKQTTAWQNLGIAQARLRHPDK
                     AIQAFQQALEHDPSMTEVSVSLARLFWMGGRHAEAIQVLLRAVEQAPDNAALLAETAA
                     LLSTAPEAELRDGERALQLAERAVQMTPQPTLQMLDALASSLAEVGRFDEAVAAAEQA
                     VAQATAAAAERSVREFTAKAELYRQHKPLRRGGQ"
     gene            18309..19142
                     /locus_tag="PLU99_02285"
     CDS             18309..19142
                     /locus_tag="PLU99_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOO15912.1"
                     /translation="MKGSFWQVLTGFGVAAAAAPALAQPAGGSEQGTSILQIFWQGIE
                     VPTYFIVAGSAVVVALVVEHFLTVRGAAIAPPEQVRKAKSLIEARDFRGCLERMKKSS
                     TFFAQVMTASLMHARHGFEAMHEAGVEKAGELSGRMFRKAEYLNILGNLGPLLGLLGT
                     VWGMIEAFGELGAAGGQASAGQLAAGISKALVNTLLGLALAIVGLGFFGLCRNRIDSL
                     TVSATVDALDLLEYFRTPGAARAAEAAAPPPPGLTPPWPGEVRGLDLSGASLPARERT
                     V"
     gene            complement(19196..20983)
                     /locus_tag="PLU99_02290"
     CDS             complement(19196..20983)
                     /locus_tag="PLU99_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15913.1"
                     /translation="MSGPRSRERLLVAVLLMIVLAGVAWTHWPALSCQALAFDDTQYL
                     RDNLLVRNPSWNSTWRFLSEVLAPSTVEGYYQPLTMISLMLNVAVGASEANRTPFQAT
                     NLALHLANTGLLLILLYLLFENVWAAAVAALLFGVHPLTVEPIPWLSERKSTLSTLFA
                     LGAMVAYVRYTHRPRAATYAALLVLYVLGLMAKPTVIGLPLMLLLMDVWPLRRIGPAG
                     SAPASRPGRTPRDEPVAARISLRKAIFEKTPLLAIAGASAIITYVSQAQTSAAVLPTE
                     SSWTRVLLILAHNIVFYIGKILWPANLTSHYPVPEPLTLAQPAVLAGVVGTAVLMLAL
                     LLSLRWTRGPLVCWLIFFVAILPTMGVIGFTNAIAADKYVYFPAVGLLILLTAGLSQV
                     LRKAAANRWAVPGVAVLALILAGAEAVGVRRYLVHWRDTEALCRRILQFAPNAGIAHM
                     ELSIELGEQGRLAESLEHAREAARLYPAYAKAQYNLGLALTLNDRLDEAIGAYALAVN
                     LNPGHWGARYNLATALLLRSRPAEAIPHLRAAADLNPLYAETYYLLGVALEQTQQYRE
                     AAAAYERTISLNPNDEESRQRLAEVRSRL"
     gene            21427..21561
                     /gene="rpmH"
                     /locus_tag="PLU99_02295"
     CDS             21427..21561
                     /gene="rpmH"
                     /locus_tag="PLU99_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOO15914.1"
                     /translation="MHYPHRISKIKRKRKCGFLVRMRTKKGRKLINRRRRMGRSIDIA
                     "
     gene            21908..23728
                     /locus_tag="PLU99_02300"
     CDS             21908..23728
                     /locus_tag="PLU99_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO15915.1"
                     /translation="MTSRMKAIRTVFSAAGFVALLLPGCDRKPNSAQPAQDAAPEPAS
                     AQVQFEIPCLTSSERAALGTALEMKLTGAYNAACGDPESVTKLATLGAMYYVHGHPEE
                     SAACFRRITELKPDDPHYWYFLGMACQAAGQPAEALAAYEGAAGIGPAYQPRLRAMGD
                     VCMPASPSRAIEYYQAALDLDPNDVLAWCGLSRAQFAHGQREEGLRSILIAANLEPYY
                     ADAQHSAAEALRGLGRHAEAEQYVQMAGVGGNAPQPKDPFGESMRRAGMEPRAVCAEA
                     MQLTDRGEVARARLILEQIRSLPGGTVAATEALGTVAAAEKKWDEAASLFSEVLAAGP
                     QAYSAKSSLGEMLIQQGNLSEAERVLREVLTEHPEHAATAKRFAALMSQLKKPHETLA
                     VMQAAVAARPFDPVRRSLLAQHYAAAGRTDAALAELRAALDLQPDYFPGRQQLALLLV
                     GERQYAAAKAEFERTLQTNPRYEDAYLYLSTIVIQADKDLRGGEKILREGLKHVPNSH
                     GLANSLAWVLATASDPAQRKPEEAVQLAERAVTATGHTQHSYLDTLGAAYAAAGRFAD
                     AARVARQAIESASRVNAPEVEQYRTRLALYEQDQPFRTPE"
BASE COUNT         4259 a         7400 c         7759 g         4325 t
ORIGIN      
        1 gccgcgagga agcggtcttt gtcggcctga tcaagcacgt cgtatggcgg cgagaggcgt
       61 gtcgaaatat cgcgattacg cggacgagtc gtatacctaa tcgcagtaaa cggacggatc
      121 gtgctcattt tgaattccct cgtcggccag tcatgcaact tctgacggcg catctgccgc
      181 cggcggccgc attgccggcg agcgcgcggc gccaggccgc ggccgtccat cgcgcaggcg
      241 catagactag cttttggtgg attggctgcg gaccgccgca ccccgggccg tcggccggaa
      301 ggaagcccaa gccctggagg aatccgcaca tgaatttcac caaacccaac tcgccaatct
      361 tctcgctcgt cgcgacaaca atccttctgt gtatcacgct gctgagcggc tgtccagagc
      421 cgcaggtgcc gcgggaggcg tccgagaact cgaacggcgt tccgccggct aacgcgttta
      481 tcccggcgga agaaatcatt cgctcgccta cgcccgtaca acttgaaaca agcggcaaaa
      541 gtgatgagga ggaaccctgg catggcggtt atggcgacgg tggcagcggc aacggcagcg
      601 gcggcggcag cggcgggtgg ggcggcacaa cgcgcgggat ggcccatatc tcgttcacgg
      661 caccgctaac accgctggcg gtgaagcctg ggggtaaggt cgcggtgcgc ttttcagtga
      721 cgctcgatcc tgatgcctac atcgccgcag ccgacctgct gattgcacgt gatcgcgacg
      781 cggatcgcaa acccgacggc gacccggtgt acgtgcagga catcaccgag cttgtcgctc
      841 cgggcgacaa cgctttcctg cttttgacgc aggtgctggc ggacctgggc ttgctccaga
      901 acggcttcgg gcgctttctg gtcggcatag cagtgcacac caacgcgggc cacacgaccg
      961 aggcttacgc ttccgggacc atcaccatcg atgcggtgcg cccgaccatg agtggtttcc
     1021 gcgccggccg cgacggactg cacgctgacc aggaagatca cctgatcgtc aatacgaccg
     1081 cggtctgggt aattgcgatg gagacttatg acaatggccc gcacagaatt aaggttctgc
     1141 tagacccaga caaagtccct ctcagcggca acgaagtgga agtactagat cagctcgtca
     1201 accctggctc gcagcaattc gttattcacc cgcgggatat tgcgccgaat aattatttct
     1261 attactttac aatcttcgat gaagtcgcgc catcgttcgc cggttacgcg gccaatagtt
     1321 cgaccggagc ccatttccgg cttggcatta cggccagatt ggttggcgat ttcaatttta
     1381 actggctcga tccagatatt cccggcgagt acagaaacgg gaccggtccc agcatgggcg
     1441 ctattctgca aggcttcaat ttcaacgacc tggccggcag ctccatgtgc agtgtgccgg
     1501 acgttaatgg cgatgcactg cccgaactgc tggtcgccgc ccgcttcggc aaaccgtacg
     1561 ttgagagtgc gcagggcatc ggtttcggcg aagcttactt gttctacggt caggccgatc
     1621 gtttacggga caagtttcaa ctgaattcgg ccggtctgga aagcgttgat tatccgacgc
     1681 tggtctttcc gggtattcgt gctccgaaga atgagacctg gacgtacgga ctggcggata
     1741 tcgcggccat tccagatatg gacggcgacg gttatccgga gttggtgttc tgcttcccgc
     1801 gggtcgaatc ggttccgctc atcctcggcc ggaacgaggc ggagatgaaa gtccagtcgg
     1861 tcccgccgga tggcgtcgtt ggggacgcca atctcgagta caacgcatac cgatacgatg
     1921 aggacgaagg caaattccgc tggtttccaa atgaggcgca gttcacgcgc ggcggcattg
     1981 tcatcgtctc cagtcacagt tatctgctgc gtcagacgga ccacctcaac cgccggggcg
     2041 accgcgtgct tgatttggcc gaggtgggcc agatttttaa ctcgcagggc gcacccgcgt
     2101 atgttccgtt catctggggt gcggtgaacg aacccgacac ggcgccctgc ggtgtcccgc
     2161 cggtgcccac gccatatgac gattggacag tctattggga catagttctg tttaatcagg
     2221 gtccgggcgg ttttgacaac gcttacacca atgcggcttt cgcccggccg caagctgccc
     2281 ctgcgaccgg cttcgagccc gatccatttc agccgcccct ggctaacgcg cgcactttgc
     2341 tgaagttgga cgtcttgaat ggagtcgtgc cccccgtcga cgacaagtgc ggcacgcagt
     2401 gcgttgtggc gaatgcgtgg cggacattcg gcaattgctc gttgcccagt ggcggcgggt
     2461 tccccggtgc ctgggacggg gtgtgcgaaa cccagagctg gcatggaagc ttcaacaagt
     2521 ccgagtccgg ccaggccatc tggaccggct tctacgggcc caacaatcct ggcgtcgcca
     2581 tcaatggtga cacggtaggc gcccgaatac tggggcagcg gcgggaggac cgcttcggca
     2641 gcagcgttgc cgccgacggc acgtacctgt acataagctc gcccgagtgc gtggccggca
     2701 gccggaaccg cgccggcgcg gtgtatcaga tgcgcacatt cgcccgcccc ggtggcagcg
     2761 cgaccacgca gacgcaactg tggatcgagc cgcgcgatcc ggcaggtaca ttcacgccga
     2821 tctggccgaa cgtggacgtc gaagatgccg cgcgccgcga ttggaccatg cccgtgccgc
     2881 accagtacat catccgcacg ggcggctcgg tccgtggcgc ggatgtgtac acgctggatg
     2941 gcgccttttg gccgccgccg ttcggtgagc cggtcggcag ctcagatcgc ttggtcaatt
     3001 acgtgtttca gggagcgggc tgtttgcagt ccgttgcatg gagcgtcaac ggcggccttg
     3061 cggccgacac gctgctgccc gcgccgggcg gctcgccctt ttggccggcg tggggaaatg
     3121 gcacggccgc gtattacgtc gaaacgacgc ggcagtacgt cggcccgcac gtgggcgcga
     3181 agatcagcca ggtgcggatc gtcggggatc tcaaccgcga cggaattccc gatttcggca
     3241 tcggttcgcc ggacgtccgc acctcgttcg gcgatccgga acaccccgac ggctgcaagg
     3301 cgggggcagt ctttatcgtg ctcgaacgtc ccaccggcgt cggcggggag gttgacctgg
     3361 gccgcatcgc cttacacccg gaccatccgc gacgtttggc gggcgtaatg attcgcggca
     3421 tgtgcggcgg ccaggtgttt gggcgcgtgt tcgatgcggc cggcgacttc aacggcgacg
     3481 gcatcgatga tgtgatcatc ggcagtgagg ggtacgacta caaccgcggg caggcggtga
     3541 tcctgttggg ttctcccaca ctggccagtc cgcaaggcgg ctggaccatc gacgaactca
     3601 tcagcgtagg gcgcggcatc cggttccgag gcgcggcggt cggcgacctg gccggcacca
     3661 atgtggccgg aataggcgac atggacggcg acggcatcga tgacgtcctg atcgccgctc
     3721 cgggcgcgtt gaacgggcgc ggcgcggtgt acctgattta cggctcgccg gctctgaccg
     3781 gccaggacgt gtcgctggga gacattggca agatcgacct gcccggcgct cgtttcatcg
     3841 gacgcgcggt gggcgacggc ctgggcggcg ggcggttggt gttcgacggc gacaatccgc
     3901 aagccgatcg ccgcatgtat ctcaacccgg gcgatccgac ggagccgtac taccgaacgg
     3961 tcgaagtcta ctcgcgcggc gtggccaaga tcggggacat tgacggcgac ggcaatgccg
     4021 acttcgccat cagttccatg ttggctgatc cgggtggcaa cgttgatgcg ggcgaagtgt
     4081 acgtaatata tggcaagagt ggtgaggagt aggggcggag gcggcgcctt aggtgcaaag
     4141 tgcgaagttc acggtgcaga atgcaaaggc ttgagcttgg gcggaggctc atcagccgcg
     4201 ttgcgcttcg ctccgcttgc ccgggcggca gcttgggcag gtgccaaaga gcgtaacctc
     4261 gtgggcgtcg acctcaaagc cggcgggcag cttcgtaccg tcggcgatcg ccagcgggca
     4321 gccgggcagg tcgtagacct gctcacattt gcggcagcgg aaatggtgat ggtggtccag
     4381 gtgggctgat tcgtagcgct tctcgccgtc cggcatgcgc agcacgtgga tctgctgggt
     4441 cgttagcagc aacttgacgg tgcggtaaac ggttgccaca cccaaccccc ggctgcggcg
     4501 ctgggcgcgc tcatgaatct gggcgattgt cagcgggccg cgcgcggcac gaattacctg
     4561 cgcgatcgtg tcgcgctgcg acgtattccg ctgctggacg gtcttcttgc tcatctgtca
     4621 taagtaatca tacgtggctg acttacccgg caccagaatt gcggttactt ccgccatagc
     4681 gacccgaaga agattaccag gacggtcgaa acctcgagcc ggcccaggag cattgcaaag
     4741 cttaacacca ccttcgcgaa ggcgggcagg ggggcatagt tgttcgaagg gccgacctct
     4801 cccagcgccg ggccgacgtt gctcatgcag gcgatgactg ctgagcccgc ggtaatcagg
     4861 tcgatgccga ccgccgtcag cagcagcgtc gctacgacaa ggaagccgac gaacagaatg
     4921 acgaagccga ggatgtcctg catgacgtct tgcggcaccg ggcggtgatc cagcttcagg
     4981 attcggacct gccgggggtg aaccagacgc ctgagctgca aaagggcatg tttgaagagg
     5041 agcagcagac gcacctgctt cacgccgccg gaagttgaac cggcgcagcc accggcgaac
     5101 atcagcgtga ccagcacggc ctggcacagg aacggccacg cttcgtagtc cgcggtcgtg
     5161 aagccgctcg tcgtgagaat ggaggtcacg gtgaaggccg agtttcgcag gttggccagc
     5221 cagttcccgc tcagcgccag gttggccagg aagacgacca gcgtggcgcc gcccaggacg
     5281 cccaagtaga agcggaactc gctgctggac cagtagttcc gcgcgcggcc cttgagggcg
     5341 tagtagtgca gcgagaagtt gacgccgccc agcagcatca ggaccagcgc gaccatttgg
     5401 ctgtagctgc tgaaggctgc gaaactggcg ttgcgcgtcg agaagccgcc ggtggcgacg
     5461 gcggtgaacg tgtggcacag ggcgtcgaag aaatccattt cgcccaacca gaacagggcc
     5521 aggcccagcc cggtcagcag ggcgtaaacg ccccacaaca ggcgggccgt atcctgaatg
     5581 cgcggcgtaa ggcgttcggc ggtcggcccc ggggcctcgg cctcgtagag ctgcatgccg
     5641 cccacgccca acagcggcag gatcgcgact cccagcacga taaagcccat accgcccatc
     5701 cattgggtaa gagcccgcca gaagagcacg cttttgggaa cggcctcgac gtcagtgaag
     5761 accgacgccc cggcggtcgt gaagccggac atggactcaa aaaacgcatt caccgggttg
     5821 gtcaggctgg cggagatgat gtagggcagg ctgccgaaca gggcgaagcc gatccaggcg
     5881 aaggtcacga cggcgaagcc ctcgcgcagc gtgatttcct cgcgttcccg gcggaagcgc
     5941 cagaacagga agccccccac caggctggtg atcgcggcgc taagcacaaa cgtcagaagc
     6001 tggccgtcgt ggtaccagat cgagaacggg atgggcagca gtaatgcggc gcccaggaag
     6061 attagcagcc cgccgagcac atagagggtc aaccggacgt tcatttgagg aagcgctcga
     6121 tgcggggtat ggcttccggc aatgcaaata cgatcacgtg gtcgccggcc tccaggtgat
     6181 cctcgccgct gggaatcact acctccgagc cgcgcacaat aatcccgatt accgcgccct
     6241 cgggaacgtc gatgtccttg agcgggcggc cgagcacggg gcttgcagtc ggcataacta
     6301 tttccagcac ttcggcgttg ccgtgttcga gggtggccag cgaggtcacg cccggcgggc
     6361 gcacgtattt gaggatggcg ctggcggtga ccaggcgcgg gctgacacag gcgtccacgc
     6421 cgagcgatgg tgccagcgtg acgaattcgt gccggtcgac gatggcgatg gcgcgtttgg
     6481 cgccgtagct cttggccagc aaggaacaca aaatattggt ttgttcgtcc tgcgtgaccg
     6541 cgacgtaggc gtcgcagccc tcgatcccct cgtgcttgag cgtatcgatg tccatgcctt
     6601 ctgcattcag cacggccacg ttatcgagtt cctcggccag ccgctcgcaa agccgggcgt
     6661 tatgttcgat gagcttgacg cggattccca ggccggccag cttgcgggcc agtgccgccc
     6721 ccacgccgcc gccgccgaga atgaagacgg ttttcgaggg gtgcttttcg aattcgaaca
     6781 agtaatcgat ggccggcaca tcgggcttgt ggcagacgaa gtaaacgatg tcgccttcct
     6841 cgatggtgtc ttcgccccgg gggatgatgg tgcgatgttt ccgggcgatg gccgtgacta
     6901 ccatgcgata caaaccgcgg atgccggcca agtcacgcag cgaaataccc gccagcgaac
     6961 tgttgcggcc gatggcatat ccgaggaaca ccacccggcc tcccgcgaac tcggccactt
     7021 cggcggccgt cgtatacgag acgatgcggt gaatctcctc ggccacgact ttttgcggat
     7081 tgatgagcag gtcgatcccg agctttccga tgcgttcggt ccagccggcg ccggtgtatt
     7141 cgacgccctt gatgcgcgcg atgatgcggc gggtgttgcg ctcgtgggcc agcaggcagg
     7201 cgaccaggtt gacctcatcc tggtcggtga cggcgatgaa caggtcggcc cggtcgattc
     7261 cggcttgctc gaggacttcg gccgtcgccc cgctgccgag cacgatcgag gcgttgagct
     7321 tggcccgcag gatgggcccc ttttccacgt cgcgctcgac cacggtgacg tcgtgtccct
     7381 ccttggacag gcggtccgcg atgtggaaac cgacctcgcc cgcgccgact acgatgacat
     7441 acatgtccaa atccaggtta gaccgcaccg tcgaccgcgg ccgagcgtcg caaaacgcgc
     7501 cagctttcgc caagctccga gggcgccgca atcaacagca cggaatagtt tagtgtaacc
     7561 gccggctgaa ccccggcaac cgggtgataa agcgataatg tgatagagtg ataaagggat
     7621 tccgcggatc gtcctttatc actctatcac tttatcacct tatcacgtga ttccggcgcg
     7681 gcacgcgcgg gctctgctac tcactacagt gggcgcgcta gaaatagagg tctctggcgt
     7741 ccgtctcgcg gatgcgctgc agccggtcga gcagttcgcg cttcaggggc ccctcttcca
     7801 ttttctccag ctcggccgcg atcagctttt cgccggcggc gcgcgtctgc ggggtggcgt
     7861 aatcgaccag gtactccaac agcgtggtca gggcgttcgg cgtgcagaag cgtttgataa
     7921 agcccgggat cgcgaattcc atgaagtgtt cgcccgtgcg gcccaggcgg tagcacgaag
     7981 tgcagaagct cggcacgtag ccgtcttcga gcaactcgcg catcaccgta tccagcgggc
     8041 ggatgtcgcc cagctcgaac tgctcacgct ccattacctg cgcatcgccg gcctcggtat
     8101 agccgccgac ctcgatgcgg ctgccggcgt caatctgcga gacgccgaag cccaacacct
     8161 cgcgccgtag cgccggctct tcgcgagcgg tcaggatcag gccggtatac ggcaccgcca
     8221 gccgcaggat ggcgatcagg cgcttgaaat cctggtcgct gaccaggtat tggtcctggt
     8281 gcatgacacc ctgggccggc cgcaggcggg ggaaactgat ggtgtgtggc ccgacgccgt
     8341 agcgttgctg caggtgcagg gcgtgcgcca ccagcccgag cacttcgaag cgccaatcgt
     8401 agagcccgaa cagcacgccg atgccgacgt cgtcgatgcc ggcctcctgg gcgcggctga
     8461 gcgcgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagca cgccgatgcc
     8581 gacgtcgtcg atgccggcct cctgggcgcg gctgagcgcg tccaggcgcc agaggtagtc
     8641 gcccttgcgc gtattcaacg ggtgatagcg gcggtacgtc tcgtggtggt aggtttcctg
     8701 gaagatctgg aaggtgccga tgtgggccgc cttgatggtg cggaatccct cgatgtcgaa
     8761 gggggcggcg ttgacgttga cgcgccggat ttcgccgtgc ccgactttga cggtgtagat
     8821 cagccgcacg gtgtccgcga tgagctcggc gttgtagcgc gcgtgttcgc cgaagaccac
     8881 gatcagccgc ttgtgcccgc ggttctgcat cgcggccacc tgcgcgcgga tttcgtcagg
     8941 gctgagcgtg cgccgtaccg cgtcggggtt cgagcgacgg aaggcgcaat actggcaatc
     9001 gttgctgcac aggttgccga tgtacagcgg agcgaagagc acgatgcggt tgccgtagac
     9061 atcgcgcttg agctgccggg cggcggcaaa gatttcttcc gtcagatccg gatcatcagc
     9121 cttgagcagc gtggcggttt cagccaggct caaagcctgc ttggagaggc tcttggcaat
     9181 tatttcgcgc acacgggccg gctcaggctg cgcgcaggcc agtacggtct caagttccgc
     9241 gtcgcggatg aaatccgtcg cgccgtcgct caacgtcatt tcaggaagtc gcatcgtcat
     9301 attttagagc atttcgggac ttcggggttt agggatcgag gaattcgaga caagaatcgc
     9361 gcgcggcgcc ttcttggccg cctgattccc tgctcccctg atcgttccct ctacgtgcca
     9421 gccagttcgg tgagccgccg tggcccgtgc cgatggtgcg tcccaatctc gtgatgcggc
     9481 gcaggatgca gccgtggcat tgctcggccg tctcgaacag gcaggccttg gccggataaa
     9541 tctcgtattt acagcggtac gcggggggcg tcaggtttgg catgatgatg ttcgcgccgc
     9601 gcatcaagcc caactcgcgg ccgctgtcgc ggttcagcgt tgccaaggcg gtggtgctgg
     9661 gcaggttggc ccgcgggcac atcaggcgcg tcaaggcgag cactttatag gtcatcagtt
     9721 ccgagttcgg gacctggccg gatggagagg gatcaaggga tccagggatc aagggatcaa
     9781 aagactgccc caacggcgtt tccggatgcg gcaggaaggg gccgacgccg atcatgtcga
     9841 ggtccagttc gcggaaaagc tccaggtcgc gcgccaggtc ctcgtaggtc tggcccggta
     9901 tgccgatcat gacgccgctg ccgacttcgt agttaagctg ccgcagcgtt cgcaggatcg
     9961 caattcggtc gctcaactgc ccattccgcg acgggtggat gcgccggaaa agctccgcgt
    10021 tcgacgtttc gaatcgcagg aggtagcgat ccgccccggc ctcgcgccag gccgccagtt
    10081 ccgccgcgct ccgctcgccc acgctgagcg tgacggccag gccggtctcc gtcttgatgt
    10141 ggcgcaccac atccgccagc cactcgctgg tgatgccgta gtcctcaccc gactgcagca
    10201 ccaccgtccc gtagccgaag ctcgcggcct ggcgggcgca ggcaagaatt tcgtctgcgg
    10261 gcatgcggta acgctcgatc aggcgattgt cggcccgcag accgcagtag tagcactgcc
    10321 gggcacaatg attcgaaaac tcgatcagcc cgcgcaggtg caccgcgtcg ccaacgtgcg
    10381 tgcggcgcac ggaatccgcg tcatgccaca gcttctccag ccgggcggag tcattctcca
    10441 gcagccagtt aattatctgc acgcggttca tgttctccgg gcggtctcgc attcttttgc
    10501 aatgtaacgg tgaaggtact gccggcgccg ggcttgctcg ttacggtcgc gtttccgccg
    10561 tacatcaaag ccagcttttt caccagcgac agccccaagc cgctgccggt gatgtggcgg
    10621 gtctggtcat tcttgatgcg cacgaaatcg cggaatagcc ggccggcgtc gtcgggcgtc
    10681 agaccgatgc cggtgtcact caccgcgatc gtcacgcgct gctcgtcgcc ggaaaggtgc
    10741 acatcaacgc ggccgccggc gcgattatat ttcaccgcgt tcgaaagcag gttgttgaat
    10801 attatttcga tttcgttgcg gtcggcgacc attaacaccg gcccgtcccc gtgcaactgc
    10861 agcgaaaggt tgtgcttctc ggcgtcgggc cggaccgtgt cgagggcgcc cttggcgatc
    10921 tcgcgcacat ccaggctcac cagctcgcgc ttcttctcgc ccgactcgac gcgcgtcatg
    10981 tccagcaggt cgaggatcaa cttgcgcatg ccgccggcac gctccaggca gcgctcgata
    11041 atgtggtcat aagccgaaag gtcgtttccg gccgtccgct ctttgagcaa gtgcagatag
    11101 ccttcaatgc cggccaacgg cgccttaagc tcatgtccca gcaccgagat gaattcgaac
    11161 cgcacgcggc gcttttcggc cgccagcttg cgcgcttgcc gctggatgac caggtgctgc
    11221 gaggccttgc ggatggtctc cttcagttcg tccggcgtga acggcttggc caggaaatcg
    11281 aaggccccgc attttgtggc ccgaatggcc gtttccaacg tcgcgaaggc cgtgatcatc
    11341 acggtcagca cctcgcgctc gtgcttgacc aggtgctcca ggacgtccag cccggtcata
    11401 tcgcccagct tgtaatccag gagcaagatg tccggggacc aggaggcgag cttctgcaag
    11461 ccctcttcac cgctggaggc cgtttcggtt tcaaaacaga cctcgccctc gacgtcggga
    11521 agctggacag tgaagtgccg cagtgcgcga gccacgctgt attgcatgcc gggctcgtca
    11581 tccacgatca gaactcgcag agtgtccatt ttacttccca ttcacagctt gtatcagccg
    11641 gcgccgtggc agcgtcacgg tgaaggtggt gccggtgggc cccgcggcgg catcggcgtt
    11701 cgactccacc ttgatgtccc cgtggtgcat tttcacgatt ccgtagctga cggccaggcc
    11761 caggcctgtg ccctcgccgg cgggcttggt tgtgaagaag ggttcgaaga tcttcttgcg
    11821 attggtgggc gggatgccga tgccggtatc agaaacgatg aagcgcacct tgtccgcatc
    11881 accgttggta aggatgctca gtttgccgcc ctgcggcatt gcggcgatag cgttgctggc
    11941 caggttcgtg agcacctgcg cgatctgatc ccggtccagt tcggcctcgg tatagtcccc
    12001 gtcgtgcgcg gtgtgaacct cgatgttctc cggaacatgc agggcccgcg tacaccgggc
    12061 gacgatctcg cgcacgtcca cgctttcgaa gtccaccttg ttcttgcggg cgaactgcaa
    12121 cagacccgat acgatcttct tgcagcgatc cgcctcgctg acgatcaagt tcaggtcctc
    12181 gcgcatttcg gagtcgaccg ccgcctcctc cagcagcagg tgcgacaaca tcagcaccgt
    12241 tccgagcggg ttattcacct cgtgcgcaat gccggccgca agctgcccca tgctggccag
    12301 tttttccgac tgcatcaagg cttcctgggt gctcgcgagt tgttcgttcg agtaggccag
    12361 ctctttgcac gtgttcttga gctgctcgat cgtgtagggc aggcacattt cactttccgc
    12421 cagccctttg acgatggcca ccgcatgctc gcggcaggtt tcgtaaccgc acgcgccgca
    12481 gttcagctca tcctccggtg cgaacttgcc catccgctcc atgaccgggg tgagctgctc
    12541 ttcggacggc atctcgatcc gctggtcatg cggagagaac gtccgccgca ggttcagctc
    12601 gcacaaggcc gaccagtgct cgtgccagta ctcgcgatcc gcatcggaca tgcggcgctt
    12661 ggcgtagcgg ctgacctgcg agcgacggct gaacagcggg gcacgcgtgc tcgagccggg
    12721 gcccatgatg cacccgttgc aggccaccac ttccagcagg cgcgcgtcga gatcgccgcg
    12781 ctcgaattcc ttgatggcct cgatgaactc ggtccgccca tcggccgcca cgaccgcgcc
    12841 cgacagcaag tcttccggaa tctgggcggc ctgcaacatg ccccggctga tggggaagag
    12901 tgagcccgta ccggcgtgcg gcgggtcgaa gtcgctgggc gtgacagact ctgcctttat
    12961 ggcatggtgc aggaacatct cccgcagctc gatgaacgtc agagtcgcgt cgacctcgcc
    13021 cgcgacgcgt tcgctcttgg cctccccttt tttcgcgata caagggccta taaagacgac
    13081 cttcaggttc ttcccataca gccgccgcag cgcccgtgca gtcgcgatca tcggcgaaac
    13141 aatcggcgcc aggctgggca ccaggttggg gtgataacgc tccacgtacc cgacgatggc
    13201 cgggcacgtc gtggatatga agcgctgccg cgggtaaagc cggagcagac gctgatattc
    13261 agccgcaacc aggtcggccc cgaaccccac ctcgtgcacg aggtgaaacc ccagcgcccg
    13321 taccatcgcg acgaagaact cgtaatcgat ttccgtaaat tccgagggaa agcttggggc
    13381 gatgatcgct gtcacccgcg ccccgctctt gagcaactct tcggccgctt gaatgccgtt
    13441 ttggacctgc ttggccccct ggctgcacat gcgcacgcag ttgccgcagc cgatgcagcg
    13501 gtcgaccagg acttccgcct gaccgctggc gatgcgaatc gccttggccg ggcattcgcg
    13561 cacgcaagtg tagcagcggc ggcagcgctc gcgaatggtc gtaactagcg gtgcattcgg
    13621 cggatccatg cgtgattccg aaagccgtgg ccggcggggg caagaccgta acattgcccc
    13681 ggtcttgacc atgcaagaac gataccatga taacctgaac tgagaggcat gccgggacgc
    13741 agtaagccag gaattcaggc gcgattacag gcctctggcc cgcgtcgccc gttagccccg
    13801 ttttctgcgg cggatgcttc cttacggtcg ttccggcggc cggcgccggc gaactgccgt
    13861 atgcgccgcc tgacgcgcat aaacctgacg ccccccaacg cgagcgttag cacccctaag
    13921 cagataaaca ggcctccgag gagttgcagc gccagcggtc cgaagaactg taccagggcc
    13981 atccccgcta cggcgaaaac cagcgccgtc cgcgcgtacg ccaggaacgt gcgctcgttc
    14041 gccagggcgt tccgctgcac ggccaggctg tcccgcaggc tcacggatgg cgccagttcg
    14101 caagtgaccg ggcgttctgc cgcggccgtg ccgtcgatga ttatgggggc cgcattcaag
    14161 gcagcccgtg gatgctcgcg ctttctgttc cgtggccgga tgtgggccat gctgttaatc
    14221 ccgtttcggt gataccttcc cgttcagccc gcccgaatag ggccttgaag atgatagaca
    14281 caaggccgcg gttccagcgg gcgggatgga atcactttcg cgtcggagcc gaccgggttt
    14341 gtccaaaacg ccccgttcgt cggcggcggg tgccttccgc ttatcggcgg gcttgttcgg
    14401 ccgctggcgg ataatcgtct cgggacggcc gtggtgcgtt gaagctggtc tggcagcaag
    14461 cgggaggcgt accagatatt cacttggcga gatccgcccg acatgttagt gtgctgnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattc acttggcgag atccgcccga
    14641 catgttagtg tgctggcgag ctgttgacct gtgtcacgtt agcgggaacg tcgattttaa
    14701 ctgactgagc gggagtcatt ctcagtccca tgctatggtc gcgttcaacc aaactggccc
    14761 cctccccggt ggacctcgac gaccgggtcg tcgcggaact gcactgcatt aaatgcggta
    14821 cgccgctacg tggagaattt gccagcggct catgtccgca atgcggacac ccgtgctcgg
    14881 attccgtgta cggcgactac ctcatttatt cggaccgcgc ggaggtggac cggctggacg
    14941 aggcggcccg ggttgtaatc tacgggggca tgctgctggg cgtcatcatc gctatcgcga
    15001 tgctgttcat ggtcacgtcg tcccagaatg taattgaggc catccgccgg gggttcgaca
    15061 cgctctgggc cggcgtgctg ctctcgccgg taatcgcgac gaccggcatg gtgctgctga
    15121 cgcgccggta ctcgatggct tattacgagg ccaagtactt ccaccggcgc ttcctgatcc
    15181 gggccggcat ctgcgcgggg ttggttctga tcctggcggg agtggcggca tatttcaggg
    15241 cgcgcgactt ccaggtgctg ctgctggccg gctgggcgac gctgccgagc gccgcattca
    15301 tgcggggctt ggcccggctg atgcggcgcg tgccgaacgt gaagctggcc agctttgcgc
    15361 aggctctctc ggccatcatc tgggtcaacg gcgtgctgag cttgctgttc aactggctgc
    15421 gcccactggg agccgagagt gcggattggg aagcgccatt gttatcgctg caaatgctgg
    15481 tgtcgtgcgg gtggatcggg ctggggatcg ccagtttgcg gctgatgatg acggtacacc
    15541 ggaattttct ggcgattcgg cgataggggc ggtcggagcc gctgtaatcc cgggcggttc
    15601 ttcactgccc gccgcgccgc agtggcttgt gctgccggta cagttcggct ttcgcagtga
    15661 attcgcggac ggaacgctcc gctgcggctg ccgtggcttg tgccactgcc tgctcggcgg
    15721 ccgcgacggc ctcgtcgaat cgtcccactt ccgcaagcga agaggccaac gcgtctaaca
    15781 tttgtagcgt gggttgcggc gtcatctgta ccgcgcgctc cgccaactgc agcgcacgct
    15841 cgccgtcgcg caactcggct tcgggggcgg tcgacagcag ggcggccgtt tcggccaaca
    15901 gggccgcgtt gtctggggct tgttcaactg cccgcaggag cacttgaatg gcctcggcgt
    15961 gccgcccacc catccaaaac agccgcgcca gactgacgct aacctcggtc attgagggat
    16021 cgtgctcgag cgcctgctga aaagcctgaa tcgccttatc cgggtggcgg aggcgcgcct
    16081 gggctatgcc aaggttctgc caagcggtgg tctgcttcgg tttttcgcgc agcacggctt
    16141 cgaactcggt gacggcggcc tgcacctcct cgtcggattt ggcctggcgc agcatgattg
    16201 cggccagatt caggcgtgcc tcagtcagct ccgggcggat ctccagggct ttacgcaagt
    16261 gctgggcggc agcgtcgtcc tggttggtct gcatcaacgc cgcgcccagg tgctgatggg
    16321 ccaaggccaa agacggctga aagtgcaggg cggtctgata cgcgcttatc gccagctcca
    16381 tctcgcgcat tgaagccagg aacaggccga agtacatgtg cgtcaatcca tcccgtgggc
    16441 ccgccttcaa ggcgttgagg aactcctgtc tggcttcctg cggccggcct agtgaaaaga
    16501 gtgtccgcgc cagcgcggtg tgttgttgag gaatgtaggg ggccaactcg atggcctgcc
    16561 ggtactcctg ctcggcgcgc tccaggtctt gcccgccata tggcgccggt gttgcgaaat
    16621 gccgccccag gatgaagtga tactcacctt caacacgctg gcgatacgtc gcactgcccg
    16681 gcggattggt ggcgatcaag ccggcgacaa ccgccacccc ggcggcaaac aaagctaccg
    16741 gcgtccatcg ccgttggcgt atgtattccg cgacactgca aactgcgaac gcggccaaaa
    16801 tgatcagcac tgggatgact ggcgcccggt aacgtgcggg acagaagaat gcgactacag
    16861 tcgccatgta gactgctgcg aaggcgaccg gaatcgtcca cgctcgccag cgcccgcgca
    16921 gcagcgccag ccccggaatg gccagtgcgg cgacgactgg gaagccgatc cagaatagcc
    16981 acgcgatcca gccggcttgc cgggcaattt gccagatggg ttgattattc gggatttcga
    17041 gtgggctcca gaacaggcgc agcttcgtga ccatgtgccc gatccacgcc ccgggcgccg
    17101 agccgatcca cttcatcgcg cgcccgaacc agtagctcga gatttcgcga tcggtaagac
    17161 tgcggcccag ttcctgctcg ggaatctgct taatgtcgac cagccagtcg cgccactccg
    17221 gccgggcctc gggcacgatc gccaggtagc cggtcgattc gggattgtta ccgatgtaga
    17281 aattgatgcc gccggttgaa gcgatcagga ccggctcgcc gcccacgaag tagttgcgca
    17341 gcgccaccgg ggcaattgga atgacgaggc ctatggttgc ggccagcggg gccagcaggc
    17401 ggcgagccgc cgagggttgc cgcgaggtcc accacgccca tagccagatc accggcgcca
    17461 ccgcgagcac attggggcgc gtaatggccg acagtcccca gaccacgcct gccgcggcga
    17521 accagagtgt cgagccgcgt cgcccggcgg ccagtagggc ccagagcaag gccgtgttga
    17581 ggcagacttc gaggccgacg gtcagcagct cgaaatcgaa gtaaaccagc ggccagtaga
    17641 gcgccgcgat cagcccggcg agcagtcccg tgcggaagcc tgccagggcg gcggccaggc
    17701 gggccatcag gtagcagttc agcgcgccga gcaggcagcc ggcgatgcgc gccacggcga
    17761 cctgtggccc ggtcagcatg tatagccagg cgagcaggta gtagtaaagc ggggcgcgca
    17821 ggtagggccg cggctccatg ccctcgccgc tggcgatgct ggcggcccac tcgtggtgga
    17881 cgcggccgtc catctgcggg aaatcgaaaa gcgggctgtt gcggtactgg ccccagaaat
    17941 acgccccgcg caccgccagc gctaccagca gaacgaccaa gagccagagg tgctggcgcg
    18001 cagtgaggcg catgttcagc aattccgcga cgcgcgcagc cgcgcggggg cgggtagttg
    18061 cggcgggctt gttgacaagc gtgcgggcga gtttgggcat ccgaaatctc tagcgcaagc
    18121 agctcaagcg gagcatctta acggcgaagc aacgagggaa cgagcggcga gagcacggtg
    18181 ggatcacttc atcaccttat cactccgttg cctgtgataa ggtgataagg tgataggtga
    18241 cgacttgggt gtttgtgcag atcgccggtt gcgggtaaat agagcatcac gggctcagcg
    18301 gagcgactat gaaaggcagc ttctggcaag ttctgaccgg ttttggcgtg gcggccgcgg
    18361 ccgcgccggc gctggcccag ccagcaggcg gttcggagca gggcacatcc atcctgcaga
    18421 tcttctggca gggtattgaa gtccccacgt atttcatcgt ggccggctcg gcggtggtcg
    18481 tagcgctggt ggtggagcac ttcctgaccg tgcgcggggc ggcgatcgcg ccgccggaac
    18541 aggtgcgcaa ggccaagtcg ctgatcgagg cgcgcgattt tcgcggttgc ctggagcgga
    18601 tgaagaaaag ctcgacattt ttcgcacagg tgatgacggc ctcgctgatg cacgcgcggc
    18661 acggttttga ggcgatgcat gaggcggggg tggagaaggc cggcgagctt agcgggcgca
    18721 tgttccgcaa ggcggagtac ctgaacattc tgggcaacct tgggccgctg ctgggattgc
    18781 tggggacggt gtggggcatg atcgaggcgt tcggcgaact gggtgcggcc ggcgggcagg
    18841 ccagcgcggg gcagcttgcc gcggggatca gcaaagccct ggtaaacacg ctgctgggct
    18901 tggcgctggc gatcgtcggg ctggggttct tcgggttgtg ccgaaatcgc atcgactcgc
    18961 tgacggtgag cgccacggtc gacgcgctgg atctgctgga gtacttccgc acgccagggg
    19021 cggcgcgggc ggcggaggct gcggcgccgc cgccgccggg gctgactccg ccgtggccgg
    19081 gcgaggttcg cggtttggat ttgagtgggg cgtcactgcc ggcgcgcgaa cgaaccgtct
    19141 gattctcaaa ccgccgattg cggcaggatg ctagccccgg gccgtcggcc ggcgttcaca
    19201 agcgtgagcg cacctcagcc aggcgctgcc ggctttcctc gtcattcgga ttcaatgaga
    19261 ttgtccgctc ataggctgcg gcggcttccc gatactgctg cgtttgctcc agtgccacgc
    19321 ccagcaggta atacgtctca gcgtacagcg gattgaggtc cgccgccgca cgcaggtgcg
    19381 gaatcgcctc cgccgggcgg ctccggagta gcaacgctgt cgccaggttg taccgcgcgc
    19441 cccagtgccc aggattcagg ttcacggcca gggcgtaggc tccaatggcc tcgtccagcc
    19501 ggtcgttcaa tgtcagagcc agccccaggt tgtactgggc cttcgcgtag gcggggtaca
    19561 gccgggcggc ctcgcgcgcg tgttccaaag attccgccag ccgtccctgc tcgcccagct
    19621 caatcgagag ttccatgtgt gcgatgcccg cgttgggcgc aaactggaga atccgccgac
    19681 acagtgcctc cgtgtcacgc cagtgcacca ggtagcgccg aacacccaca gcttcggccc
    19741 ccgccaaaat tagcgccagc actgccacgc ccggcaccgc ccagcggttc gccgccgctt
    19801 tccggagcac ctggctcaat cccgccgtta gcaatatcaa caagccgacg gccggaaaat
    19861 acacgtactt gtcggccgca atcgcgttcg tgaagccgat gacgcccatg gtcggcagga
    19921 tggcgacgaa gaagatcagc cagcacacaa gcggtccgcg cgtccagcgc agcgacagca
    19981 acagcgccag catcagaacg gccgttccca caacgcctgc caggaccgcc ggctgcgcga
    20041 gggtcagggg ctcgggcacg ggataatgcg aggtgaggtt cgccggccat aggattttgc
    20101 caatatagaa cacgatgttg tgcgccagga tcagcagcac gcgcgtccac gagctctcgg
    20161 tcggtagaac ggccgccgaa gtctgggctt gcgagacgta cgtgataatc gcggacgcgc
    20221 ccgcaatggc cagaagcggc gtcttttcga atatcgcctt gcgaagactt atgcgtgccg
    20281 cgacgggctc gtcgcggggc gttcgtccgg ggcgggacgc tggtgccgag ccggcaggcc
    20341 caatgcgtcg cagcggccag acatccatca acagcagcat cagcggcagt ccaatgacgg
    20401 tcggcttggc catcagcccc agcacgtaca gcacgagcag ggcggcgtaa gtggcggcgc
    20461 gcgggcgatg cgtgtagcgc acataggcca ccatcgcgcc cagggcaaac agggtgctaa
    20521 gcgtgctctt gcgttcgctc agccagggaa tcggttccac cgtcagcggg tgaacaccga
    20581 acagcaacgc cgccacggct gcggcccata cgttttcaaa cagcaggtac agcagaatca
    20641 gcagcaagcc cgtgttggcc aagtgcagtg cgagattcgt ggcttgaaac ggggtgcgat
    20701 tggcctcgct cgcgccgacc gccacgttga gcatcagcga gatcatcgtg agcggctgat
    20761 agtacccctc gaccgtcgaa ggcgcgagca cctcggacag gaagcgccag gttgagttcc
    20821 agctcgggtt gcgaaccagc agattgtcgc gcaggtactg cgtatcgtcg aacgccagag
    20881 cctggcagct caatgcgggc caatgcgtcc aggccacgcc cgccagcacg atcatcagca
    20941 gtaccgcaac tagaaggcgc tcccgcgacc gcgggccaga caccccaggc tcagctagca
    21001 ccgccgggcg gtccggacga gcacacgaaa cgggcctcct gcgcgtcatg ctggttcacc
    21061 ctcttgccgg cggtggcgtg gcgcattgat catcccgtag aaccggtaca cgtacctggc
    21121 gccgagctgc cgccgtcgcc gttcaatgta ccgtgccggg gctgttcgcc aaagccgatt
    21181 tcttacgatc ggccggcctt ccggctgctg cgactcgccc ggccttgccc gagcgcaccg
    21241 tccacaaatc cggtgcgata cggcttaagg acgcgcttgg cgcgtatcgt ttaatttggc
    21301 atactttggg attcgtcgga agccaggccc gcaagcgacg gggctggcga gcgtttgatt
    21361 tcaccgcggc cggccggccc gctgaggcgg ggccatcggt cgccctgaag aataggtgcg
    21421 tgagctatgc attacccgca cagaatcagc aagattaagc gtaagcgcaa atgcggtttc
    21481 ctggtccgga tgcgcaccaa gaaagggcgc aagctcatca accgccggcg ccgcatggga
    21541 cgctcgatcg acatcgcctg attctgcgaa cggggcgccg tgcgcggggg catggcccac
    21601 gcagacgaat tcaggcgcct gcccgcgttt tgactttttc gggcttgcga tcgcgccgcg
    21661 cgcgaccgtg agggctttct gtcgccgtcg caccgttacg aagaccctgg cgaaagaggc
    21721 tgctgacccc cttcacttgc ccgtttcagt tcgcgtttag tgacagccga tcttatcgga
    21781 agatgcgcag catgcgtgcc gcatacgccc cgcgccggcg cgcaccgggt atcgagccga
    21841 tacgctttcg gctatggttg caacaacacg agcgaagctt tttcctctct agcaggatac
    21901 gtggcgtatg acgtctcgaa tgaaggcgat caggacggtg ttttccgcgg ccggcttcgt
    21961 cgcactattg ctccccggct gcgatcgcaa gccaaattcg gctcagcctg ctcaagacgc
    22021 ggcaccggaa cctgcctccg ctcaggtaca gttcgaaatc ccctgcctta ccagttccga
    22081 acgcgccgct ttgggtacgg cgctcgagat gaagctcacc ggcgcttaca acgccgcgtg
    22141 cggtgaccca gagagcgtca ccaagctggc gacgctggga gccatgtatt acgtgcatgg
    22201 ccacccggaa gaaagtgctg cctgcttccg ccgcattacc gaactcaagc ccgacgatcc
    22261 ccactactgg tacttcctgg gcatggcttg ccaggcggcc ggacaacccg ccgaggctct
    22321 ggccgcgtac gaaggggcag ccgggatcgg accggcttac cagccgcgat tgcgcgccat
    22381 gggcgacgtt tgcatgcccg ccagccccag ccgcgctatc gagtattacc aggcggccct
    22441 cgaccttgat cccaacgatg ttctggcctg gtgcggacta agccgggcac aattcgcgca
    22501 tggacagcgt gaagagggtc tgcggagcat tctgatcgcc gccaacctgg agccctatta
    22561 cgccgacgcc cagcacagcg ccgccgaagc cctgcgaggc ctgggccggc atgcggaggc
    22621 tgaacaatac gtccagatgg ccggtgtggg agggaacgcg ccgcagccga aggatccttt
    22681 cggtgaatcg atgcgccgcg cgggaatgga accgcgtgcg gtgtgcgccg aggccatgca
    22741 gcttacggac cgcggcgagg ttgcgcgggc gcggcttatt ctggagcaga ttcgctccct
    22801 gcccggaggc accgtcgccg ctactgaagc gctcgggact gtcgcggccg cggagaagaa
    22861 atgggacgag gcggccagtt tgttttccga agtactggcc gcgggcccgc aggcttacag
    22921 cgccaaatcc agcctcggcg aaatgctgat tcagcaagga aacctgagcg aggccgagcg
    22981 cgttctgcgc gaggtgctga cggagcaccc ggagcatgcg gcgaccgcca aacggtttgc
    23041 ggctctgatg agccagctca aaaagccgca cgagacgttg gcggtgatgc aggccgccgt
    23101 ggccgcacgc ccgttcgatc ccgtgcggcg cagcttgttg gcgcagcact acgctgccgc
    23161 ggggcggacg gacgccgctc tggccgaact gcgggcggct ctggacttgc agccggacta
    23221 cttcccagga cggcagcaac tggcgctgtt gcttgtaggg gagcgccaat acgccgcggc
    23281 gaaagcggaa ttcgagcgca cgctgcaaac caacccgcgc tacgaagacg cgtatttgta
    23341 cctttcaaca atcgttattc aggccgacaa ggacctgcgc ggcggtgaga agatattgcg
    23401 cgaggggctg aagcacgtgc ccaactcaca cggcttggcg aacagcttgg cgtgggtctt
    23461 ggcgaccgct tccgatccgg cgcagcgcaa gccggaagag gccgtgcaac tcgcggagcg
    23521 cgccgtcacc gcgaccggcc acacgcaaca ctcctacctg gacacactcg gcgccgccta
    23581 cgccgcggcg gggcgcttcg cggacgccgc ccgcgtcgcg cggcaagcaa tcgaatccgc
    23641 cagccgcgtc aacgccccgg aggtcgagca ataccgcacc cggctggcgc tgtacgagca
    23701 agaccagccg ttccgcacgc ccgaatagaa ggacgctctt gaagcagaat cagcccttcg
    23761 ccgcgccgaa cagaggaacg ggccagtctt gaatcgtcaa tttaagtcgt cgtgaatctg
    23821 aatcctaagt cgtcctggtg gccgcgatct gctaggggaa aatgaggcgg gcgcgatagg
    23881 gcgggaatac gcccccaacg ggattcgaac ccgtgtcgcg ggcttgaaaa gcccgtgtcc
    23941 tag
//
LOCUS       DAOSYS010000020        23928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1746_length_23928_cov_5.370921, whole genome shotgun sequence.
ACCESSION   DAOSYS010000020 DAOSYS010000000
VERSION     DAOSYS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23928
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1746_length_23928_cov_5.370921"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLU99_02305"
     CDS             <1..362
                     /locus_tag="PLU99_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15916.1"
                     /translation="ARSITAALQQIKHELNTHVTPADIQAACRAVGHITGFGVASFNV
                     LAAPAVGVLVGVVARCCERRRRRVTPIACNNCGYDLTGNTSGRCPECGCVTESLRNEE
                     CEVGPTDQTLETFRGPI"
     gene            complement(387..>1544)
                     /locus_tag="PLU99_02310"
     CDS             complement(387..>1544)
                     /locus_tag="PLU99_02310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO15917.1"
                     /translation="LLDREFARTRRYGGEVALIMLDLDHFKAVNDTYGHEVGNRVLQA
                     TACYLERMVRDTDLVARYGGEEFAIICPQTRADDAAHLALRIRRGFAEEVRVPECQLR
                     VTATLGVVCSSDPEIDSVIDLINRADQALYAGKAQGRNRVVRAEQMAQPGEPAIESGE
                     IERLRKQVMVLSMQAKNLCLQSIWSLVQALEARDPLTACQSRNVTFYVGCLVKSAGWP
                     ESLACAAGNAAMLHNLGKIGVPDRILQKPDVLTPAEARVMRQVPLITCKILEPLRIFE
                     TEMLIVRHLRERWDGTGYPAGLAGPTIPLGSRMLAIAEAFDALTSDRAYRQRRTLDAA
                     LAEVKAQAGKQFDPQFSDLLLKVVNEQRDAWQERIDQALKVAAQQMSLASD"
     assembly_gap    1545..1644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2122..2967
                     /gene="panB"
                     /locus_tag="PLU99_02315"
     CDS             2122..2967
                     /gene="panB"
                     /locus_tag="PLU99_02315"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOO15918.1"
                     /translation="MVGPRPQRIDLDHLRRMKAARQKIAMLTAYDFRMAAAAEAAGVH
                     SLLIGDSMGTVLLGHESTRGTPLSLMVTLGEAVRRGAPSVFLIGDLPFECISVGGEAV
                     LSGARRFRDEAGCDAVKVEVGPGEEDLIAGLARAGINAVAHLGLRPQQVLTPDGYRSQ
                     ARDAESIADLVAQARRMVAAGAAMLLLEAVPNEASQAVVAALEVPVIGCGAGPACDGH
                     VLVTEDMLGLGAPRPPRFVPQHAQLGEQVARALSQYVEDIAAGRYPGPEHVYHMRGAT
                     HAAKP"
     gene            3030..3869
                     /locus_tag="PLU99_02320"
     CDS             3030..3869
                     /locus_tag="PLU99_02320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO15919.1"
                     /translation="MTNEHALSRCPQCGLGWWEWADFEPAELYGQGYFQSAECCQGYN
                     DYAALEPGLRRTARDRLERIDSVRPGWDRSNVSRPARRLLDVGCGTGVFLDEAARLGW
                     EVEGLEVSEYAANVARRRNLRVQSGAIETANFAAASYDCITLWDVIEHLRDPAGVLAS
                     AAAALRPGGVLALSTGDLNATVARLSGRRWHLFTLPEHLFFFTTASLRALLARVGLTV
                     ARVTREVYWAPLGYLVERLAKMFGRPAPPSCQALNGIILPATLFDVIGIYAVRRAARQ
                     AGT"
     gene            3886..4695
                     /locus_tag="PLU99_02325"
     CDS             3886..4695
                     /locus_tag="PLU99_02325"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOO15920.1"
                     /translation="MHTPLSAENLLPNGPLRRVGHKFLAFDELDSTNAYGLAHAGGLP
                     DGTVIVAEYQSAGRGRLGHSWHAPRGASILLSVLLKEPADSLLVKRSTMLAALAACEA
                     VEAACGARPLLRWPNDLLLSGRKLAGVLVESSPLPKAAGQTPLRALVLGIGINCLQQR
                     GHFSGDLAETATSLEIELPHAVDRAGVGRCLLESLDRHLSELGAAPGSTNLVAAWKSC
                     CNDLGRCVTLRHDRKTYSGTVLDVTDEGDLLVQLDRGGRRCFAAATTTRIA"
     gene            4858..5763
                     /locus_tag="PLU99_02330"
     CDS             4858..5763
                     /locus_tag="PLU99_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO15921.1"
                     /translation="MSLLVTGSIALDTLETPAGRAENVLGGSAIYFALAASQFAPVRV
                     VAAVGEDFDRGLLCCFDGKCVDTLGIEFRKGSKSFRWHGRYAGTMNEAQTVAVDLNVL
                     GEGGAPIPPAFRDSRFVFLANTHPRLQREFAAQLPQAQLIVCDTMNLWIENEPAELRR
                     TIAGVHGLVLNEAEARQLSGKSNLVTAGRELLKLGPRFVVIKKGEHGSILISQDDLCI
                     MPAYPTEKVVDPTGCGDSFAGAMMGYLAAQGRWDAPTLRAAIARGTVVASYVIESFSV
                     EAAAKLTRADVDRRLGELSRLARFE"
     gene            complement(5814..6686)
                     /locus_tag="PLU99_02335"
     CDS             complement(5814..6686)
                     /locus_tag="PLU99_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO15922.1"
                     /translation="MQPKPGKSARTDVRRRAVGCRLLAAPAIRQHTAPVLTCSLQSGS
                     NGNCTYVEAGGVRLLFDAGISGRQAQQRLASNGRDIRGVHGLILSHSHSDHTRAAGIY
                     QRLFHLPIYAAPGTQRMLAACAGPLYDVRSFEPGDTLTFGPVRVHTLPTPHDAPDSIA
                     FVIEYAHRRLGLLTDLGHPFDGLANALSELDAVYLESNYDPHLLATGSYPAWLQARIA
                     GPGGHLSNAEAAELLRACGRGRLQWAALAHLSEQNNEPELALETHRAILGPDFPLLVA
                     NRYRASQLLHVHAV"
     gene            7015..9246
                     /locus_tag="PLU99_02340"
     CDS             7015..9246
                     /locus_tag="PLU99_02340"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15923.1"
                     /translation="MRTLLNGVERVLSGLRSDVRHTSRRLAALLLIAALAPQAPAECD
                     PQEVGRLLASDAAAYDHFGYSVAISGDTAVVGAWSDDHAAGNGAGSAYVFVRSDGVWK
                     QQARLTASDAATDDYFGLSVAISGDTAVIGAPWNDHEGGTDAGAAYVFTRSHGFWFQQ
                     AKLTASDAADFDIFGYSVAVSGDTAVIGAYGDDHAGGNSAGSAYVFVRSGETWIQQAK
                     LTAADAAPYDEFGVCVAVSGDTAVVGAWLDDGAAIDTGSAYVFVRTDEVWTQQAKLTA
                     SDAEACDQFGSSVAVSSDTALVGAIYDDHGGLTDAGSAYVFVRSESAWLQQAKLTASD
                     AATGDEFGVSVAVSGDSAVVGAWLGGDSGATDAGSAYVFVRADDDWTEQAELTGSNTA
                     AGDYFGNSVALSGNTAVIGAPCTDHAENTDAGSAYVFDLNCQGVCCFSNGSCAKMIEA
                     ECDGVWHFEWADCAEAHCPPPTGACCLPGGICQVMTKAECAAEGGVYRNHGSSCEPDP
                     CSERMGACCLTDRGCQVGTEDYCVAAGGVYQGDDISCAAAQCPHAPCANILKLRVEGE
                     GAVEAAPALDIYDCGAVVELSATPAPCWHFVGWEGPVADSSGVATVTMYGNKTVTAVF
                     AIDKHVLEVSVEGAGRVALSPEGGIYDCGSTVQLTATPEPGWHFAGWRGDAAGENAGS
                     SILMDGERYATAVFEADETDDASSGQEAPQSALCSGISGALIGLTLIGLMRSGRTARR
                     RST"
     gene            9303..10871
                     /locus_tag="PLU99_02345"
     CDS             9303..10871
                     /locus_tag="PLU99_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOO15924.1"
                     /translation="MLKRLLPTRCAMSAKGMSSPALQVGIVAAAVAGLLCQLVGCTQA
                     SRLDLDRTTGGNAALFGQFRAYDGQSGEPLTFDDVVRRCAAADVVLFGEEHSDAVCSQ
                     LEAQLLAALAARARPVALAMEFFERDTQACLDQYLEGQLDEPAFRKATRQGRAYVLAH
                     RPLIEMCRAACIPVIAANAPRRLVREYRQSDLEYAEFRAGLDSSDQQWLPPENEYLGG
                     EYHERFIETMTHHDEATPGGAPGMPAPSIPPGMPPASPNAPGMAGAPHSTGAARPGSA
                     QSAPTQPDATSAPVDASAPVDAAVDASTGVAASSAEEVALLAELGASLDRLAEAAAEI
                     SRVLEEFEAAASQPVTQPASPGTPVTSPEAPTEPGAGDASPASQPAETMPQPMADPEA
                     FYRAQLLWDEAMASSVAEARACLPRHRVMLIVGGFHVIRDGGTAQKLRCRRPNDRIVT
                     VAYSGTREPALAFDPSDCNAGDIIIYGIQPPPKPTSDKPAGMPPMTQPAPASMPMTQP
                     AGVEAATISRPQGE"
     gene            11345..13543
                     /locus_tag="PLU99_02350"
     CDS             11345..13543
                     /locus_tag="PLU99_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO15925.1"
                     /translation="MTRSAVEMPVTQPVTQPSTPDLEPLFCPQSVALVGASRTPGSVG
                     HAVMHNLIHGGYTGVVYPVNPKAKSILGVRCAPRVKAIDDQVDLVVLAVQPHLVEPTV
                     IEAADHGTRHFVVITAGFKEVGGEGKEREDSLKAVARERGLTIVGPNCLGVINTDPTV
                     RMNATFGRGMPKAGALGLISQSGALCTALLDYAKGIGMGFSRFISFGNKAAVSELDLL
                     RSLARDPYTRVILMYVEELSSGQAFVETCYEITHGRNPKPILAIKTGRTSEGAAAAAS
                     HTGSLAGSDEVYDAIMTQAGVLRVESVKELFDYAEIFADPILPAGRRTAIITNAGGPG
                     IMATDACIRNGMQLARFQEYTLKSLRYQMPPTANIKNPVDVIGDAKQDRYRTALDAVT
                     ADEGVDQVLVVVTPQTMTDVTAIADVIRESKSYCGKPIVGSLMGLVDVGAGVQLLQQN
                     GVPTYAFPEDAMRSMAAKCRFAEWTRSKTLGYQHFEVDQAGAARLMAEEAAGGRLQLV
                     EYKALEVLKHYGFPVVPYQLARSPEEAAAAAKAMGFPVVLKISGPKILHKTDVGGVRL
                     NLKDEAEVQAAYTQMIETVRSKMKPDVEIWGVLVQKMLPKGKEIILGMTRDPRFGPLL
                     MFGLGGIYTEALRDVSFRLAPLRQDVPPEMIRSIRSYRLLEGVRGEKPSDVKAVAECL
                     LRLSQLVTENPNIKELDINPLMVYARGEGAMAADARIILSPDVEPNGNGK"
     gene            13650..15536
                     /locus_tag="PLU99_02355"
     CDS             13650..15536
                     /locus_tag="PLU99_02355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO15926.1"
                     /translation="MPKDWTQRYAHKIVTAEEAIGKIRPGDRVYIGSACGEPQELVRV
                     MGKMGDQLTDTEIVHVLTLGVAPYTDPRFATNFRANAFFIGNSVREAVNEARADYTPV
                     FLSQVPGLFRSHRLGIDVALVTVSPPDEHGHFSLGVAVDIGKAAVESAKIVVAQVNRR
                     MPRTLGDSFVHIDQLDFLVPHDEPLLEWPVGGGEPDEVTRQLAANLARLLTDGSTLQL
                     GIGRVPDAVLGLLTNKHDLGIHTEMFSDGVLKLVEAGVITGKHKTLHRGKIVGSFAAG
                     SQALFDFLDNNPQIEMHPSEYTNSPFVIAQHDNMVAINSALEVDLTGQVVADSLGQML
                     YSGLGGHADFMRGAALAKNGKPVIAVPSTAMTPEGLKSRIVATLQPGAGVITTRGDVH
                     YVVTEYGSAYLHGKTMRERAMSLISIAHPDFRSELLLAAKRRRIVYPNQIMPPPRAPY
                     PAEYETTFKLRNGQEAFVRPIRPDDEPLMRDMFYEFSEQTIYLRYHASVKAMPHNKLQ
                     VFCNVDYDTEMALIALTGTPGNERVIGVGRYMTDAAKQSAEVAFTVSDDFQRHGLGTY
                     LFERLVEIAKSKGIRFFHAYVLVENSGMLKIFHRSGLNIETSKEGDVVKVSMTLPETK
                     ERKV"
     gene            15555..17564
                     /locus_tag="PLU99_02360"
     CDS             15555..17564
                     /locus_tag="PLU99_02360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO15927.1"
                     /translation="MKKAMRTRGKQKGTAAPAAVVNVSAGEIHPGPSNGGHRRNAELA
                     GWEKRYPEKIRTAAQAVACVQPGQRVYIGSNAGEPQTLVEALSAREDLLDTEIVHILT
                     LGVAAYAQPRLGQRFRTNAYFIGANVREAVQAGRADYTPIFLSEIPALFRTGRVPIDV
                     AMISVSPPDSHGYCSYGVSCDIGRAAAETARLVIAEVNMQMPRVLGDCFIHMRDIDLA
                     VPSDRPVLEAPQAKLDAISQRIGRHIANLIEDGSTLQLGIGAIPDAVLHYLGDFKDLG
                     IHTEMFSDGIIPLIERGVITNAAKNIQRGKIVMSFCMGTQKLYDFIHNNPSVECHPTE
                     YTNDPFVIAQNDKMVSINSAIEVDLTGQVCADSLGVLFYSGIGGQVDFVRGAARSKGG
                     KPIIALPSTTADGIISRIVPTLKPGAGVVTSRGDVHYIVTEHGVAYLHGKSIRERALA
                     LIQIAEPKFRPWLLAEAKQRKLIGPETIELTLESLVQTEQLEFWVRLKDQTRIFMRPM
                     KLTDLALLRSMFYELAVDISRYPYLWTLVEMPAEERAEALTIDYDTDMCLVALTGAGG
                     NEKVLGIAHYRKDIHTNFAHALFVVRADWQNRGIGRALLEKLVEVARARGIAGFTADV
                     VESDQSVLRLFHNCGYAVEGDARDGYIRLTVPFGKQGPRPKASAP"
     gene            17662..18438
                     /locus_tag="PLU99_02365"
     CDS             17662..18438
                     /locus_tag="PLU99_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOO15928.1"
                     /translation="MDELRRKLDELKTIGRGYAAPAAPPAAPARLHDVVGGCELCCEG
                     VACWQIETPLEDACARHGFAIPERLAAFATAPASQSLGDIAPHAAVVIDIETGGFAGT
                     EIFLVGLIPLERRPLCVIQWLARDYAEEAALLHALARLGPQRNTWVTFNGKSFDGPFL
                     SDRAALHRVPLRLPENHFDLLHHARRRWKGELPNCRLRTLEQQILGWKRIGDVDGWDI
                     PDLFHHFLRTGNAAPLQPVLHHNQLDLISCTELLLRMVGT"
     gene            18753..20270
                     /locus_tag="PLU99_02370"
     CDS             18753..20270
                     /locus_tag="PLU99_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOO15929.1"
                     /translation="MSQRTGIHIDPRRNEPLYKQIYDELVSRIRAGEYPTGHRLPPSR
                     NLARELATHRNTVARAFDALEQSGFVYSVVGRGTFVAAQPGSAAPAAEPLPTLPWNAL
                     LSRAADAEPLARLDRMVHSVQRTDLVNLTSLYPSADLLPANALRRCLDHVLRTCPAES
                     LSFAPRYGIPRLREAIAGLLRAAGVPVGPDDLLITSGSQQALDLFARALINPGDVLLA
                     EEYTYTGVLTAFAAAGATIVGVSGDAEGPDMEVLRRLGRNGAKAFYLMPNCGNPTGAS
                     VSLARRKQLVAWSHEFGIPLIEDDYGADLDLQGGPRIPALRALDADVLYVGTFSKRLI
                     PALRVGFLACPQLLRRRLVMLKHSMDLGTSALIQQALAEFIERGYLRAHLRRIIPAYR
                     SRRDALEAGLREHLPPGIEWQQPRRGGFLWLKLPPAYDTELVFEEAQRHGVLVSPGAL
                     HSVHRPQHGGIRLTFCAEPPERLAEGARRLGSALRELAARATLESSAAAPGMDGV"
     gene            20494..21525
                     /locus_tag="PLU99_02375"
     CDS             20494..21525
                     /locus_tag="PLU99_02375"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOO15930.1"
                     /translation="MAKANLDWANLAFGYSKTDYNIRYFWKNGAWDQGRLTQDETLDL
                     HIAATCLHYGQECFEGLKAFETRSGETVVFRLDENARRLARSCEKIHMPAVPEELFCE
                     AVYRVINANRRFVPPYGTGASLYIRPIVIGTGPQIGVRPATEYTFMVLVMPVGPYFKT
                     GFKPVSLLIEENVDRAAPLGVGDVKVGGNYAAGLRASLDAKGKGFADALFLDAKEKKY
                     IDECAPANFFAITKDDQYVTPHSESILPSITNKSLITLAEDMGLRPQRRPIPVEEIFE
                     FKEAGCCGTAAVITPVGSITYRDRKAVYSKDGQPGKICTALYNELTAIQLGDAPDRYR
                     WLFPVPQTK"
     gene            21865..22386
                     /locus_tag="PLU99_02380"
     CDS             21865..22386
                     /locus_tag="PLU99_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15931.1"
                     /translation="MSLRAPYVIALALVLLVSGLMGCPRFPEEIEIEKGDTIIVEGPP
                     TFADAGSVLGPTATIIADVSLIPIEVLPDVTVLTFENLTGVDIVISYFAEGFPEAVLV
                     PDFTFVSIEYVPCLLDVQLDFEDDFDPVTGLYLGSFDLSDILLIEGLDYFCGDEILFT
                     FDPDFVFVDVFPL"
     gene            22603..>23928
                     /locus_tag="PLU99_02385"
     CDS             22603..>23928
                     /locus_tag="PLU99_02385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO15932.1"
                     /translation="MANRPAQVCRYRATVEALAHGKLVNGQRATAQRHLEECEPCRKL
                     FRDQNVECFPTFRNYTIVERVGEGGFGVVYRAVSHTKQRTEALKVLFGKTALREAYFQ
                     NEVHLVAKLRHPNIATLFDAHLTTPPLFYTMEFISGQQLGEYFRTHRPPLAQRIALLQ
                     KVVAAISYAHSQGVVHRDIKPQNILVDARGEPIIVDFGIGKKLGLPCNDHEPNGSPEG
                     FLGTFGFVAPEQAAGQPVDERADIYALGALLYHCVTGEPARSGNDAAHLTQIFQQREI
                     TRPAGLAAIIARCVDPTPEERYPDCAALLQDIAAYLSGGRIQAETNTNPARSVARAAA
                     YLLQHRPLPVCGFLVLLMASLLATFAWRADARWALPGSSAQTAALITFTPDTIEAIRT
                     GQIGADLPGLNVENRKSLRLLQGQLLERLAGAQPRVVVIDGYYPDCQPQF"
BASE COUNT         4182 a         7487 c         7878 g         4281 t
ORIGIN      
        1 tggctcggag tatcaccgcc gccctgcagc aaatcaagca cgaactcaac acgcacgtca
       61 cgccggccga cattcaggcc gcttgccggg ccgtcggcca catcactggg ttcggtgtcg
      121 cctccttcaa tgttttggct gctccggcgg tgggtgtgct ggttggcgtt gtggcgcgat
      181 gctgcgagcg gcgccgacgc cgcgtgacgc ccatcgcctg caataactgt ggctatgatc
      241 tcactggcaa cacgtctggg cgctgtcctg agtgcgggtg cgtcacggaa agcctgagaa
      301 acgaggaatg cgaagttggc cccacagacc agacgcttga aacctttcgc gggccaatct
      361 gaggagaaaa acacttaccc cgcccgctag tcgctcgcca ggctcatttg ttgcgcggcc
      421 actttcaatg cctgatctat tcgctcttgc caggcgtcgc gttgctcgtt caccaccttg
      481 agcagcaaat cagagaactg cgggtcaaat tgcttccccg cctgagcttt gacttccgcc
      541 agggccgcgt ccagcgtgcg ccgctgccga tacgcccgat cactggtgag cgcgtcgaag
      601 gcttcggcga tcgccaacat gcgcgagccc aacggaatcg tcggaccagc cagcccggcc
      661 ggatagcccg tgccgtccca gcgctcgcgc aaatgccgca cgatcagcat ctccgtctcg
      721 aagatccgca gcggctccag aatcttgcag gtgatcaacg gcacctgccg catgacgcgc
      781 gcctcggccg gtgtcagcac gtccggcttc tgcaggattc gatcgggcac gccgatcttc
      841 cccaggttgt gcagcatggc cgcgttgccg gcggcgcagg ccagcgattc gggccagccg
      901 gcgcttttca ccaggcagcc cacgtaaaaa gtgacgttgc gcgattggca ggccgtcagc
      961 ggatcgcgcg cctccagcgc ctgcaccagc gaccagatgc tctgcaggca caaattcttg
     1021 gcctgcatgc tcagcaccat cacctgcttg cgcagccgtt caatctcgcc ggactcgatg
     1081 gccggttcgc ccggctgcgc catttgctcg gcgcgcacca cgcggttgcg accctgggct
     1141 ttccccgcgt agagggcctg gtcggcccgg ttgatcaaat caatgaccga gtcgatctcg
     1201 gggtccgacg aacacacgac gcccagagtg gccgtaacgc gcagttggca ctccggcacg
     1261 cgcacttcct cggcaaagcc gcgccggatg cgcagggcca ggtgggccgc gtcgtccgcc
     1321 cgggtctgcg ggcagatgat ggcgaattcc tcgcccccgt agcgcgccac caggtcggta
     1381 tcgcgcacca tgcgctccag gtagcacgcc gtcgcctgca gcacgcgatt gcccacttca
     1441 tgcccgtacg tatcgttgac ggccttgaag tggtccaggt ccagcatgat cagcgccact
     1501 tccccgccgt aacgccgagt gcgggcgaac tcgcgatcca acaannnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnacgctg agctgctcgc gcagcaggcg gcgtagcaac
     1681 agctcgcgct gggcggggtc gtcttcgata atgcaaacat ctatcggggc ttcttccata
     1741 gttttccgga caaaagtccg tcggcgcttt cgacataccg ggcccagcct gtggacttgg
     1801 cttccagccg gccgcggtta tcagccatcg cgcatgttca ggggcattgt cctatcggcc
     1861 atatcggcca ccgcttcgtt ttggttacgc tctgagcggc cggaatttcg caaacgcgca
     1921 aaccggcccg tggacggctc atgcgcacgg cgggcggctt ttatcgcccc gccgcagggc
     1981 cgtcctggaa ggcgacgcga gccgtatgat taaggatgct tttgcgacgc cttttttggc
     2041 tgtatcgttt agcgttttgt cggaccgccg ggccagcgcg gcacagcggc ctacaagaag
     2101 tttaagaaat ggaggtagct catggtcggc ccgcgacccc aacgtatcga cctggatcat
     2161 ctgcgacgca tgaaagccgc ccggcagaaa atcgccatgc tgacggccta cgacttccgc
     2221 atggccgcgg cggccgaagc ggccggcgtg cattcgctac ttatcggcga ttcgatgggc
     2281 acggtgctgc tggggcatga gagcacgcgc ggcacgccgc tgtcgctgat ggttacgctg
     2341 ggcgaggccg tgcgccgcgg ggcgcccagc gtgtttctca tcggtgatct gccgtttgag
     2401 tgcatttcgg tcggcggcga ggcggtgctg agcggggcgc ggcgcttccg cgatgaggcg
     2461 ggctgcgatg cggtcaaggt tgaggtcggt cccggcgaag aggacctgat cgccggtttg
     2521 gcgcgggccg gcattaacgc cgtggcgcac ctgggcttgc gaccgcaaca ggtgctcacg
     2581 cccgacggat atcgctcgca ggcgcgcgac gccgagagca ttgccgacct cgtcgcgcag
     2641 gcccgccgga tggtggcggc cggggcggcc atgctgttgc tcgaagcggt gccgaacgaa
     2701 gcttcgcagg cggtggtggc ggcgctcgag gtgccggtga tcggctgcgg agcgggacca
     2761 gcgtgcgacg ggcacgtgct tgttaccgag gacatgcttg gcctgggcgc gccgcgtccg
     2821 ccgcgtttcg tgccgcagca tgcgcaactg ggtgaacaag tggcccgtgc tttgagccag
     2881 tatgtggagg acatagccgc gggccggtat ccgggcccgg aacacgtcta ccacatgcga
     2941 ggcgctacgc acgccgccaa accatgaccc gccttacgcc gcccaaagcg tgcattgcct
     3001 gcggtagcca gccccggcgc agcctgcgtt tgaccaacga gcatgcgctg tcgcgctgcc
     3061 cgcaatgcgg cctcggctgg tgggaatggg ccgatttcga gcccgccgaa ctgtacgggc
     3121 agggttactt ccagtctgcc gaatgttgtc agggctacaa cgattatgcc gccctcgaac
     3181 cgggcctgcg tcgaaccgca cgcgaccggc tggagcgcat cgacagtgtg aggccgggtt
     3241 gggaccggtc gaacgtctcg cggccggccc ggcgtttgct cgacgtcggc tgcggcaccg
     3301 gagtgtttct cgatgaggct gcgcggctcg gctgggaggt tgaaggcctg gaggtcagcg
     3361 agtacgccgc caacgtggcg cggcgacgga atctgcgcgt tcaaagcggc gctattgaga
     3421 cagccaactt cgccgctgca tcctacgact gcataacgct ctgggatgta attgaacacc
     3481 tgcgcgatcc agccggcgtg ttggccagcg cggcggcggc cttgcggccc ggtggcgtgc
     3541 tggctttgtc taccggcgat ctgaacgcaa ccgtcgcacg gcttagcggg cggcgctggc
     3601 acctgtttac gctgcccgaa catttgttct tcttcacgac ggcgagcctg cgcgcactgc
     3661 tagcccgggt tggcctgacc gtggcacgtg tcacgcgcga ggtgtactgg gcgccgttgg
     3721 gatatctcgt tgaacgcctg gcgaagatgt tcggccgacc ggcgccaccg tcatgccaag
     3781 ccctcaatgg cataattctt cccgcgactt tgtttgacgt gattggaatc tacgcggtcc
     3841 gccgcgccgc ccgacaagcg ggtacctgag gcttacgcag cagcaatgca cacgcccttg
     3901 agcgccgaaa acctcctgcc gaacggccca ttgcggcgag tcggccataa gtttctcgcc
     3961 ttcgacgagt tggacagcac gaatgcctat gggttggcgc acgccggcgg cctgccggac
     4021 ggcacggtca ttgtcgccga gtaccagagc gccgggcgcg gccggctcgg acacagttgg
     4081 catgcaccac gcggggcgtc gatcctgctc agcgtgttgc tcaaggagcc cgccgactcg
     4141 ctgctcgtca agcgtagcac aatgttggcc gccctggccg cttgtgaggc cgtcgaggct
     4201 gcttgcggcg ctcggccgct tctgcgctgg cctaatgatc tgctgctctc cggccgcaag
     4261 ctggcggggg tactggtcga gtcctccccc ctaccgaagg cagccgggca gacgccactg
     4321 cgggcactgg ttctcgggat cggtatcaac tgcctccagc agcgcggaca tttcagcggt
     4381 gatttggccg agaccgccac gtcgctggag atcgagctgc cgcacgcggt ggaccgggcc
     4441 ggagtcggcc gctgcttact cgagagtctc gatcgccatt tgagcgagct cggcgcggca
     4501 cccggatcga caaacctggt cgcagcttgg aaatcgtgct gcaatgattt gggccgctgc
     4561 gttacactcc gccacgaccg caagacctac agcggcaccg tgctggatgt caccgacgaa
     4621 ggcgatctgt tggttcagct cgatcgcggc ggccgccgtt gcttcgcggc cgccacgacg
     4681 acgcggatcg cgtgaacgaa gaatcgcgta aacgagaatc cgagcccgac gcgctagcga
     4741 gggccttgca ttattcccgg ggcaacacgc cccggcagga aaatccgagc ccgaagcgcc
     4801 agcgagggca ctacgtaatt ccccggggcg acacgccccg gctctgctgg aggttctatg
     4861 tctcttctag ttaccggatc gatcgcgcta gacactctcg aaaccccggc ggggcgggcc
     4921 gaaaacgtcc tgggcggctc ggcaatctac ttcgcactgg ccgcctcgca gttcgccccg
     4981 gtgcgcgtcg tcgcggccgt cggcgaggac ttcgatcgcg gcctgctgtg ctgctttgat
     5041 ggcaagtgcg tcgacacgct gggcatcgaa ttccgcaaag gcagcaagag cttccgctgg
     5101 cacggccgat atgccgggac gatgaacgag gctcagacgg tggccgtcga tttgaacgtg
     5161 ctgggcgaag ggggcgcccc gataccgccc gcgttccgcg acagccgctt tgtgttcctg
     5221 gccaacacgc atccgcgttt gcagcgtgag ttcgcggctc agttgccgca ggcgcagttg
     5281 atcgtctgcg acacgatgaa cctgtggatc gagaacgaac cggccgagct gcgccgcacc
     5341 atcgccggcg tacacgggct ggtgctgaac gaggccgaag cgcgccagct cagcggcaaa
     5401 tcgaacctgg tgacagccgg tcgcgagttg ctcaagctcg ggccgcgttt cgtggtaatc
     5461 aagaagggcg agcacggcag cattctcatc tcgcaagacg acctgtgcat catgccggct
     5521 tacccgacgg agaaggtggt ggacccgacc ggctgcggcg atagcttcgc cggcgcgatg
     5581 atgggctacc tggccgcaca gggccgctgg gacgcaccca cgctgcgggc cgcgatcgcc
     5641 cgcgggacgg tggtggcgtc ctacgtgatc gagtcgttct ccgtcgaggc ggccgcgaag
     5701 cttacgcggg cggacgtcga ccggcgcctg ggcgaactgt cgcggctggc gcggttcgag
     5761 tagccatctg acgctccaca ttccgaagcg acacgccccg gctctgcgga cggtcacacg
     5821 gcgtgaacgt gcagcagttg cgacgcccgg taacggttgg ccaccagaag cggaaagtcg
     5881 gggccgagta tggcgcggtg cgtttcgagg gccaactcgg gctcgttgtt ctgctcgctg
     5941 aggtgggcca acgcggccca ttggagccgc ccgcggccgc aagcgcgcag caactcggcg
     6001 gcttcggcat ttgacagatg gccgccgggg ccggcgatgc gggcttggag ccaggccgga
     6061 tacgaaccgg tggccagaag atgcgggtcg tagttgctct ccaggtagac ggcgtccagt
     6121 tcactgagcg cgttcgcaag cccgtcgaag gggtggccga ggtcggtcaa aaggccgaga
     6181 cggcgatgag cgtattcgat cacgaacgcg atgctgtccg gggcgtcgtg cggcgtgggc
     6241 agtgtgtgga cgcggacggg gccgaacgta agcgtatcgc cgggctcgaa gctgcgcacg
     6301 tcgtacagcg gaccggcgca ggcggcgagc atgcgctgcg tgccgggcgc ggcgtagatg
     6361 ggcaggtgga aaagccgctg gtagatgccg gccgcgcggg tgtggtccga gtggctgtgc
     6421 gaaaggatga ggccgtggac acccctgatg tcacggccgt ttgaggcaag ccgctgctgg
     6481 gcctgacgcc cgcttatgcc ggcgtcaaag agcaggcgca cgccgccggc ttcgacgtag
     6541 gtgcagttgc cattggagcc ggattgcagg gagcaggtca gcacgggggc agtatgttgc
     6601 cggatggcgg gggccgcaag aaggcggcac ccgacggccc ggcggcgcac gtccgtgcgg
     6661 gcggatttcc ctggttttgg ttgcaatccg cagctttttt ggttgcagtc cgccgttttc
     6721 agttgcagtc cgcccttcga atcggatagt ctctgtttaa ccatcgaata tggtgcgcgc
     6781 cgacccaccc gggttcggaa cgcaactact gaagcaattg gagtgagcgc agacgttcgg
     6841 tcatgtgcgg gaaggctgag tacgttttga ggcaagatag gcggtagaat cccggcccgg
     6901 aagcgtatcg acgtcgggct gacaccaggc gcggccaacc accgcgtccg gaaatcgccc
     6961 caatttcgtg gccgcctcgg ctgctgtccc gactgaacgc ggaggaggat tgttatgcgg
     7021 acattgctga atggcgtcga acgggttttg tcgggccttc ggagcgacgt gcggcacacg
     7081 tcgcgccggc tcgccgcgtt gctattaatc gcggcactgg ccccgcaagc gccggccgaa
     7141 tgcgatccgc aggaggtggg ccggctactg gcctccgacg ccgcagcgta cgaccatttc
     7201 ggttattcgg tggccatctc cggtgacacg gcggtggtcg gggcctggtc cgatgaccac
     7261 gccgcgggaa acggcgcggg atcggcctac gtgttcgtcc gctcggatgg agtctggaag
     7321 cagcaggcca ggctgaccgc ttccgatgcc gcgaccgacg attatttcgg gctgtcggtg
     7381 gccatctccg gcgatacggc ggtgatcggg gcgccctgga acgaccacga gggaggaact
     7441 gatgccggcg cagcgtacgt ctttacccgg tcacacggat tctggttcca gcaggccaag
     7501 ctgaccgctt ccgacgctgc ggactttgac atatttggct actccgtggc cgtttcgggc
     7561 gatacagccg tgatcggggc gtatggcgac gaccacgccg gaggcaacag tgccggttcg
     7621 gcgtacgttt tcgtgcgctc gggcgagacg tggatccaac aggccaagct taccgctgca
     7681 gacgcggcgc cttacgacga attcggcgtc tgcgtggctg tctcgggcga cacggcggtg
     7741 gtcggggcgt ggttggacga cggcgcagca atagataccg gatcggccta cgttttcgtc
     7801 cgaactgacg aagtctggac gcagcaagcc aagttgaccg cgtccgacgc cgaggcgtgc
     7861 gatcagttcg gcagctccgt ggccgtctcc agcgacaccg cactggtcgg ggcgatctac
     7921 gacgaccacg gcggattgac agatgccggg tcggcctacg tttttgtccg ctccgaaagc
     7981 gcgtggttac agcaggccaa actgaccgcc tcggacgccg cgaccggcga cgaattcggc
     8041 gtcagcgtgg cggtctccgg tgattcggcg gtggtgggag cgtggttagg cggtgactcc
     8101 ggagcaacag acgccggatc ggcctacgtt ttcgtgcgcg cggacgacga ttggaccgag
     8161 caagccgagc tgaccggttc caacaccgcg gcgggcgact acttcggcaa ctctgttgcg
     8221 ctctccggca acacggcggt aatcggggcg ccctgcaccg accacgccga aaacactgat
     8281 gccggttcag cgtatgtatt cgatctcaat tgccagggcg tttgctgctt ctcaaacggc
     8341 tcatgcgcca aaatgattga agccgaatgc gacggggtct ggcatttcga gtgggcggat
     8401 tgcgccgagg cacactgccc accacccaca ggcgcatgct gcttgcccgg cggcatctgc
     8461 caggtgatga cgaaggccga atgcgcggcg gagggcgggg tctatcgcaa tcacggttcg
     8521 agctgcgagc cggacccgtg tagcgagcgc atgggcgcat gctgcttgac tgatcgtggt
     8581 tgccaggtgg gaacggagga ctactgcgtt gcggccggcg gcgtctacca aggtgatgac
     8641 atctcctgcg cggcggcgca gtgtccgcac gcgccgtgtg caaatatcct gaagttgcgc
     8701 gtagagggcg agggtgcggt cgaggcggct cctgctcttg atatctacga ttgcggggca
     8761 gtggtagagc taagcgccac gccggcgccg tgctggcatt ttgttggctg ggaggggccg
     8821 gtggccgaca gttcgggcgt tgccactgtc acgatgtacg ggaacaagac agttacggct
     8881 gtgttcgcaa tcgataagca cgtgctcgaa gtctcagtgg agggcgcggg ccgggtggcg
     8941 ctcagtccgg agggcggcat atacgattgc gggagtacag tgcaactaac ggccacgccg
     9001 gagcctggct ggcactttgc cggctggcgc ggagacgctg cgggcgagaa tgccggcagc
     9061 tccattctca tggatggtga gcggtacgcc acggccgtat tcgaggcgga cgagacggac
     9121 gacgcgtcat ccggacagga ggcgccgcag tcagcattgt gttccggtat ttccggcgcc
     9181 ttaatcggct tgacgctcat tgggctgatg cgaagcggca ggacggcgcg gcggcgcagc
     9241 acctgataat gctgagcagt ttcaaatttc gcgttttgag ttcgggtgcc ggaggagagg
     9301 gcatgctgaa gcgcttgttg ccgacccgtt gcgccatgag tgcgaaaggt atgtcgtcgc
     9361 cggcgctgca ggttgggatt gtcgcggccg cggttgcagg gctgctctgc caactggtgg
     9421 gttgcacgca ggcttctcgc ctggacctgg accggactac cggtggcaat gctgcgcttt
     9481 tcggtcagtt ccgcgcgtac gacggtcaaa gcggcgaacc gctcacgttt gacgacgtgg
     9541 ttcggcgctg cgcggctgcg gatgtcgtcc tcttcggcga ggagcactcg gacgccgtgt
     9601 gcagccagct cgaggcgcag ctcctggccg cactggccgc gcgggcgcgg ccggtcgccc
     9661 tggcgatgga gtttttcgag cgcgatacgc aggcttgcct ggaccagtac ctcgaagggc
     9721 agcttgacga acctgccttt cgcaaagcca cgcgccaggg ccgcgcttat gtgctggcgc
     9781 atcggccgct gattgaaatg tgccgcgcgg cctgcattcc cgtgatcgcg gccaatgctc
     9841 cgcgccggct ggtacgcgaa tatcgccagt cggaccttga atacgccgag ttccgcgccg
     9901 gtttggattc gtccgatcag cagtggctac cgcctgaaaa tgagtacctg ggcggagagt
     9961 accatgagcg cttcatcgag acgatgacgc accatgacga agccacgccg ggcggcgcgc
    10021 ctgggatgcc ggccccatcg attcctccgg gcatgccgcc cgcttctccg aatgcgcccg
    10081 gcatggcagg cgcaccccat agcaccgggg cggcacgccc cggctctgct caatctgctc
    10141 cgacgcagcc tgacgccacg agcgctcccg ttgacgcgag cgcgcccgtc gacgcggccg
    10201 ttgacgcaag cacgggtgtc gcggcgagct cggccgaaga agttgcgctg ctcgccgaac
    10261 tgggcgccag cctggatcgt ttggctgagg ccgccgcgga gatcagtcgc gtgctcgagg
    10321 agttcgaggc ggccgcgagt cagccggtga cgcagccggc cagccccggg acgcctgtaa
    10381 cctcgccgga agcgccaaca gagccggggg cgggagatgc gtcgcccgct tcgcagccgg
    10441 cggagactat gcctcagccg atggccgatc cggaagcgtt ttaccgggct caactccttt
    10501 gggatgaggc catggcgagt tcggtggcag aagcgcgggc ctgcctaccg cggcaccggg
    10561 tgatgttgat cgtcggaggg tttcacgtca tccgggatgg cggcaccgct cagaaactcc
    10621 gctgccgccg gccgaacgac cggatcgtga ccgtggcgta ttcgggtacg cgggagcccg
    10681 ccctggcctt cgacccgagc gactgtaatg ccggggacat catcatctac ggcattcagc
    10741 cgccgccgaa gcccacgagc gacaaaccgg ccgggatgcc gccgatgacg cagccggcgc
    10801 cggcgtctat gccgatgacg cagccggccg gcgtcgaggc cgcgaccatc tcccggcctc
    10861 aaggggagtg acccgcctgg cgttaaggat cgcggcggcc agccctgata ggggcagaac
    10921 gcgaacaggg ggcgttcccg caggtgtagt caggccgggt gggcgtccgg gcgtgcgccg
    10981 ctaacggatt taagtacctg tcgccagcga gtttacggct atcatctaaa gttaccggtc
    11041 gttcaggtga tcactcaaat aatgaacaaa gcggtggcac ggcagttgcc ggttctgagc
    11101 aggcgcggtg agcagccgtc cgcggaccgc cgcctgcgta ccaacggatt ggtccctctc
    11161 ggcgagcggc ttgggtggct ccgcaaaccg cgggggcgct tacgtgacga gcttgaggaa
    11221 aaaggcgaaa atgagccggg gcgaacgatt ccgcgaaagc cagaccgttg ccgaaccgtc
    11281 agcgcctgac cgacagggcg cgaaagggaa gagtgctgaa atttctcgca ggagcctggg
    11341 tgctatgacg cgaagtgccg ttgagatgcc agttacccaa ccagttaccc aacctagtac
    11401 gccggacttg gagccgctgt tctgcccgca gtctgtggcg ctcgtcggcg cgtcccgtac
    11461 gccgggctcg gtcggccacg cggtgatgca caacctgatc cacggcggct atacgggggt
    11521 cgtttacccg gtcaacccga aagccaagag cattctgggg gtgcgctgcg cgccgcgcgt
    11581 caaagccatc gacgaccagg tagacctggt cgtgctggcg gtccagccgc atcttgtcga
    11641 gccgaccgtc atcgaggcgg ccgaccacgg cacgcggcac tttgtggtca tcaccgcggg
    11701 gttcaaggaa gtcgggggcg aaggcaagga acgcgaagac tcgttgaagg cggtggcccg
    11761 ggagcgcggc ctgacgattg tgggccccaa ctgcctggga gtcatcaaca ccgacccgac
    11821 ggtgcggatg aacgcgacgt tcgggcgcgg catgccgaag gccggggcgc tcgggctgat
    11881 ctcgcaaagc ggcgcgctgt gcacagccct gctcgactac gccaagggca tcggcatggg
    11941 cttttcgcgc ttcatcagct tcgggaacaa ggcggccgtg agcgagttgg atctgctgcg
    12001 gtcgctggcc cgggatccgt acacgcgcgt catcctgatg tacgtcgagg agctgagcag
    12061 cgggcaggcc ttcgtcgaaa cctgctacga aattacgcat ggccgcaatc ccaagccgat
    12121 tctggcgatc aaaaccgggc ggacttcgga aggtgcggca gccgcggctt cgcacaccgg
    12181 ctcgctggcc ggatcggatg aggtttatga cgcgattatg acccaggccg gcgtgctgcg
    12241 ggtggaatcc gtgaaggagc tgttcgacta cgccgagatt ttcgcggatc cgatactgcc
    12301 ggccgggcgc cgcacggcga ttatcaccaa tgccggcggg ccgggaatca tggcgaccga
    12361 tgcctgtatt cgtaacggca tgcagctcgc gcggttccag gagtacacgc tcaagtcgct
    12421 gcgttaccag atgccaccca cagccaacat caagaacccg gtggacgtga tcggagacgc
    12481 caagcaggac cggtatcgca cggctctgga cgcggtcacg gctgacgagg gcgtggacca
    12541 ggtgctggtc gtcgttacgc ctcagaccat gaccgacgtt acggccattg ccgatgtcat
    12601 ccgcgaatca aagagctatt gcggcaagcc gatcgtgggc tcgctgatgg gcctggtgga
    12661 cgtgggggcc ggcgtgcaac tgctgcagca gaacggcgtc ccgacgtacg cgttccctga
    12721 agacgcgatg cgctcgatgg ccgccaagtg ccgcttcgcc gagtggacgc ggtccaagac
    12781 gttgggctac cagcatttcg aggttgacca ggcgggcgcg gcgcggctca tggcggaaga
    12841 ggccgcgggc gggcggttgc agcttgtgga gtacaaggca ctggaggtat tgaagcatta
    12901 cggtttcccg gtcgtgccct atcagttggc tcgcagcccg gaggaagccg ccgcggcggc
    12961 caaggcgatg ggcttcccgg tggtactgaa gatttccggg cccaagattc tgcacaagac
    13021 cgacgtgggc ggcgtgcgtt tgaacctcaa ggacgaggcg gaagtccagg ccgcgtacac
    13081 ccagatgatc gaaacggtcc gcagcaagat gaagccggac gttgaaatct ggggcgtgct
    13141 ggtgcagaag atgttgccga aaggcaagga aatcattttg ggcatgacgc gcgatccgcg
    13201 tttcggaccg ctgctcatgt tcggcttagg cggcatctac accgaggcgc tgcgtgacgt
    13261 ttcgttccgc ctggcgccct tgcgccagga cgtgccgccg gagatgatcc gcagcatccg
    13321 ttcgtaccgg ctgctggagg gcgtgcgcgg cgagaagccg tcggatgtca aggccgtggc
    13381 tgagtgcctg ctgcgcctgt cgcagctcgt gacggagaac ccgaacatca aggaattgga
    13441 tatcaacccg ttgatggttt atgcccgcgg cgaaggtgcg atggcggccg atgcgcgcat
    13501 tattctgagc ccggatgttg aaccgaacgg aaacggcaag taaatacgca tgggagtgca
    13561 acctccggtt tgcgtgaggt aaggacgcga ggcagagctt gctctactga agacgcccgg
    13621 cggggccttg cactccctgg gagacgctga tgccaaaaga ctggacccaa cgctacgcgc
    13681 acaaaatcgt cactgctgaa gaggcgatcg gcaagattcg ccccggggat cgggtataca
    13741 tcggttccgc atgcggtgag ccgcaggaac tggtgcgagt gatgggcaag atgggcgacc
    13801 agctcacgga caccgagatc gtgcacgtgc tcacgctggg cgttgcgccc tacaccgatc
    13861 cgcgttttgc caccaacttc cgcgccaacg ccttctttat aggcaatagc gtgcgcgaag
    13921 cggtgaacga agcacgggcc gattacacgc cggtgttcct ctcgcaggtc cccggcctgt
    13981 tccgctcgca tcgcctgggc atcgatgtgg cgctggtcac ggtcagcccg ccggacgagc
    14041 acggccactt cagtctgggc gtggcggtgg acatcggtaa ggccgcggtt gaatcagcca
    14101 agatcgtggt ggcccaggtc aaccgccgaa tgccgcgcac gctgggcgat tcgtttgtcc
    14161 acatcgatca gctcgacttt ctggtaccgc atgacgaacc gctgctcgag tggccggtgg
    14221 gcggcggcga gccggacgaa gtgacgcggc aactggcggc gaacctggca cggctgctca
    14281 cggacggctc taccttgcag ttggggatcg ggcgcgtccc ggatgccgtg ctgggcttgc
    14341 tgacgaacaa gcacgatctg ggcattcaca ccgagatgtt ctccgacggg gtgctgaagc
    14401 tggttgaggc gggcgtaatt accggcaagc acaagactct gcataggggc aaaatcgtcg
    14461 gcagcttcgc ggcgggcagc caggcgttgt tcgacttcct ggacaacaac ccgcagatcg
    14521 aaatgcaccc gtcggagtac acgaacagcc cgttcgtgat cgcccagcac gacaacatgg
    14581 tggccatcaa ctcagccttg gaagttgatt taaccggtca ggttgtggcc gactcgctcg
    14641 gccagatgct gtacagcggt ttgggcgggc atgccgactt tatgcgcggc gccgctttgg
    14701 ccaagaacgg caagccggta atcgcggtgc cgagcacggc catgacgccc gaaggcttga
    14761 aatcgcgcat cgtcgccacg ctccagccgg gcgccggagt cattacgacg cgcggcgatg
    14821 tacactacgt agtgaccgaa tatggtagtg cctatctgca tggcaaaacc atgcgcgagc
    14881 gggccatgtc gctgatcagc atcgcgcacc cggatttccg ttcagagctg ctgctggccg
    14941 ccaagcgccg gcgaatcgtg taccccaacc agatcatgcc gccgccgcgg gcgccgtatc
    15001 cggctgaata tgaaaccacc ttcaagctgc gcaacggcca ggaagctttc gtgcggccga
    15061 ttcgcccgga cgacgagccg ctgatgcggg acatgttcta cgagttcagc gagcagacca
    15121 tctacttgcg ctatcacgcc tcggtcaaag ccatgccgca caacaagttg caggtgttct
    15181 gcaacgttga ctacgacacg gagatggcct tgatcgcgct gaccggcacg ccgggcaacg
    15241 aacgggtgat cggggtcggg cgttacatga ccgacgccgc caagcaaagc gccgaggttg
    15301 ccttcacggt ttcggacgat ttccagcggc acggtttggg gacgtatctc ttcgagcggc
    15361 tggtcgagat tgccaagagc aagggaatcc ggttcttcca tgcctatgtg ctggtcgaga
    15421 attcgggcat gctcaagatt ttccaccgtt ccggcctgaa catcgaaact tccaaggaag
    15481 gcgacgtggt caaggtttcc atgacgctcc cggagaccaa ggaacgcaaa gtctaatacc
    15541 cgcacggaag tgacatgaaa aaagcgatgc gcactcgcgg caaacaaaaa ggaactgcgg
    15601 ccccggctgc ggtggttaat gtttcggccg gcgaaatcca tccgggtcct tccaacggag
    15661 gtcataggcg caacgcggaa ctggccggct gggagaagcg ttaccccgag aagattcgca
    15721 cggcggcaca ggcggtcgcg tgcgtgcaac cgggccagcg tgtctacatc gggtccaacg
    15781 cgggagagcc gcagacgctg gttgaggcgc tctcggcgcg tgaggatttg ctggacacgg
    15841 aaattgtcca cattttgacg ctgggggtgg cggcctatgc ccagccgcga ctgggccagc
    15901 gtttccgcac gaatgcctat ttcattgggg cgaacgtgcg cgaggcggtc caggcggggc
    15961 gggccgacta cacacccatc tttctctcgg agattccggc cctgttccgc accgggcgcg
    16021 tgccgatcga cgtcgccatg atctcggtct ccccgcccga cagccatggc tactgcagct
    16081 atggcgtgtc ctgcgacatt gggcgggccg cggcggagac ggcccgcctg gtcatcgcgg
    16141 aagtgaacat gcagatgccg cgcgtgctgg gagactgctt cattcacatg cgcgacattg
    16201 atctggcggt tcccagcgat cgccccgtgc tggaagcccc gcaggccaag ctggacgcga
    16261 tttcgcagcg catcgggcgg cacattgcca acttgatcga ggacggttcg acgctgcagc
    16321 tcgggatcgg cgccattccg gacgccgtgc tgcactacct gggcgacttt aaggacctgg
    16381 gcatccacac cgagatgttc agcgatggaa tcatcccgct gatcgaaaga ggcgtcatca
    16441 ccaacgccgc caagaacatt cagcgcggca agatcgtgat gagcttctgc atgggcacgc
    16501 aaaagctgta tgacttcatc cacaacaatc caagcgtcga gtgccacccg accgagtaca
    16561 ccaacgaccc cttcgtcatc gcgcagaacg acaagatggt ttccatcaac tccgccatcg
    16621 aggtcgatct gacggggcag gtctgcgcgg actcgctcgg cgtgctcttc tacagcggca
    16681 ttggcggaca ggtcgacttc gtgcgcggcg ccgcccgctc gaaaggcggc aagcccatca
    16741 tcgccctgcc ctcgaccacg gccgacggca ttatttcgcg tatcgtgccg acccttaagc
    16801 cgggcgcggg cgtggtcacc agccgcggcg acgtgcacta catcgtcacc gagcacggcg
    16861 tggcttacct gcacggcaag agcattcgcg agcgcgccct ggcgctcatc cagattgccg
    16921 agccgaaatt ccgcccctgg ctgctggcgg aggccaagca gcgcaagctg atcgggcccg
    16981 agacgattga gctgacgctc gaatcgctgg tccagaccga acagttggaa ttctgggtgc
    17041 gcctcaagga ccagacgcgc atcttcatgc ggcccatgaa gctgacggat ctggcgctgc
    17101 tgcgctccat gttctatgag ctggccgttg acatcagccg ctatccgtac ctctggacgc
    17161 tggtcgagat gccggccgag gagcgcgcgg aagccctgac catcgactac gacaccgaca
    17221 tgtgcctggt ggcgttgacc ggggctggtg gaaacgagaa agtcctgggc atcgcgcact
    17281 atcgcaagga catccacacc aacttcgccc acgccttgtt tgtcgtccgg gccgactggc
    17341 agaaccgcgg catcgggcgt gccttgctcg agaagctggt cgaagtcgcg cgagcccgcg
    17401 gcatcgccgg cttcacggcg gatgtcgtcg agtcggacca aagcgtgctg cgattgttcc
    17461 acaattgtgg ttacgcagtc gaaggcgacg cacgcgacgg ctatatccgc ctgacggtgc
    17521 ccttcggaaa gcagggtcca cgcccgaagg cgagcgcccc ctgagacggc acaagcgtgc
    17581 gcatgttcgt gcggacgccg gaacggagac ggggcggcgc ggcaggctgc cgtggattgc
    17641 ccgcttcggt atgatccagg gatggacgaa ctccgccgca aactcgacga actgaagacc
    17701 attggccggg ggtacgccgc gccagcagca ccgccggcgg ctccggcccg gctgcacgat
    17761 gtagtgggcg gctgcgagtt gtgttgcgaa ggcgtcgcgt gctggcaaat cgagacgccg
    17821 ctggaggatg cttgcgcccg gcacggtttt gcgatcccgg agcgcttggc tgccttcgct
    17881 actgctcccg ccagtcagtc gctgggtgac atagcccccc atgcggcggt cgtcatcgac
    17941 attgaaaccg gcggcttcgc gggcaccgaa atcttcctgg tcggcttgat tccgctcgag
    18001 cgccggccgc tttgcgtcat ccagtggctg gcccgcgatt acgctgagga agccgccctg
    18061 ctgcatgccc tggcgcgcct cggcccgcag cgcaatacgt gggtcacgtt caacggaaag
    18121 agttttgacg gcccgtttct gtcggatcgc gcggccctcc atcgcgtgcc gctgcgcctg
    18181 ccggagaacc acttcgactt gctgcatcat gcgcggcggc gctggaaggg cgaactgccg
    18241 aactgccggc tcaggacgct ggaacagcaa atcctgggct ggaagcgcat cggagacgtg
    18301 gacggttggg acatcccaga cctgttccac catttcctgc gcaccgggaa cgcggccccg
    18361 cttcagcctg tgctgcacca caatcagctc gatctgatct catgtactga gctgcttttg
    18421 cgcatggttg ggacttgaag gccggctcgc gggtaagcgg cggcaaaacg acgccggcca
    18481 gcgcgggtgc catgcccaag ccccgcgacg gcggggcgcg ggcatgccgt tgccgcttgc
    18541 cgttaccagt cgccgcggcc cggacgccgt tcacccagga acgcgccccg gccgcacaaa
    18601 ttccgttttt atggggccgg tcgccggggc ggcttttcgg ctctgctatt ggcctaattg
    18661 tccacagaaa atcgagcatt ttgcttgcat tgtccgccgc ttggtgccat acttttaggg
    18721 tgtaggcagt ggccaaatgg acggccggcc caatgagcca aagaaccgga attcatatcg
    18781 acccgcggcg caatgagccg ctttataagc aaatttacga cgaactcgta agccgaatcc
    18841 gcgccggcga gtatcccacc ggccaccgtc tgccaccctc gcgtaacctg gcgcgtgagc
    18901 tggccactca tcgtaatacc gtcgcacggg ccttcgacgc gctggagcaa tccggattcg
    18961 tctactccgt tgtcggacgc ggcacctttg tcgccgcgca acccggctcc gcagcccctg
    19021 cggcggagcc gctaccgact ttgccgtgga acgcgctgtt atcgcgcgcc gcagatgccg
    19081 agccgctggc gcgcctcgac cgcatggttc acagcgtgca gcgcacggat ctcgtcaatc
    19141 tgacgagctt gtatccgtcc gccgacctgc tgccggcaaa cgccctgcgc cgctgcctgg
    19201 atcacgtgct gcgtacttgc ccggccgagt cgctgagctt cgccccgcgt tacggaatac
    19261 cgcggttgcg ggaggcgatt gccgggctgt tgcgtgcggc cggcgttccg gtcggccccg
    19321 acgacctgct gataaccagc ggcagccagc aagccctgga ccttttcgcg cgggccctga
    19381 tcaacccggg cgatgtcctg ctggccgagg agtacacgta caccggcgta ttgacggcct
    19441 ttgcggcggc tggcgcgacg attgtgggcg tgtcaggcga tgccgagggg cccgacatgg
    19501 aagtcctgcg gcgcctgggg cgaaacgggg ccaaggcgtt ctacctgatg ccgaattgcg
    19561 gcaacccgac tggcgcgagc gtcagcctgg cgcgtcgcaa gcagctcgtg gcctggtcgc
    19621 acgaattcgg aattccgctc atagaagacg actacggcgc tgatttagat ctgcaaggcg
    19681 gcccgcgcat cccagccctg cgggcgctcg acgccgacgt cctgtatgtc ggcacgttca
    19741 gcaaacggct catacccgcg ctgcgcgttg gattcttggc ctgcccgcag ctcctgcggc
    19801 gccggctggt catgctcaag cacagtatgg atttggggac ctcggctctg atccagcagg
    19861 cgctggccga attcattgag cggggttacc tgcgtgctca cttgcggcga ataattcccg
    19921 cttatcggtc gcgccgggac gcgctcgaag cgggcttacg tgaacatctg ccgcccggca
    19981 ttgagtggca acagccgcgc cgcggcggat tcttgtggtt aaagttgccg ccggcctacg
    20041 acaccgaact ggtgttcgaa gaggcccagc ggcacggcgt gctggtttcg cccggagcgt
    20101 tgcacagcgt gcaccgcccc cagcacggcg gcatccgcct gaccttctgc gccgagccgc
    20161 ccgaacggct ggcggaaggg gcgcgccgcc tgggctctgc cttgcgtgaa ttggcggcgc
    20221 gcgccacgct ggaaagttca gcggccgcgc cgggaatgga tggcgtttag gagccggcca
    20281 ggccgcggcc tgtcagccgg atcagccggc ggtcgcaagc gagtgtccca gcggcacatg
    20341 cggctgcggg aagaagtggc tagtgcgata attgtttacg gatatatttc aggagccgtt
    20401 actatgacca aatctgcttc gatcggctcg gcgaaccggt acaatgagcc agtagaaaaa
    20461 tcgtttcccc aagatggagg cggggaagtg cgcgtggcga aagcgaattt ggattgggcc
    20521 aacctggcgt tcggttattc caagacggac tacaacattc gttatttctg gaagaacggc
    20581 gcgtgggacc aggggcggct gacgcaggac gagacgctgg acctgcacat tgccgccacc
    20641 tgcctgcact acgggcagga gtgctttgaa ggtctcaagg cgttcgagac gcgtagcggc
    20701 gaaacagtcg ttttccggct ggatgagaac gcccgccgcc tggcgcgttc ctgcgaaaag
    20761 attcacatgc ccgcggtgcc ggaggagctg ttctgcgagg ccgtgtaccg cgtcatcaat
    20821 gccaaccgcc gcttcgtccc gccgtacggc accggggcca gcctgtacat ccgccccatt
    20881 gtgatcggca ccgggccgca gattggggtg cgccccgcga ccgagtacac cttcatggta
    20941 ctggtgatgc cggtcggccc gtatttcaaa acgggcttca aaccggtttc gctgctgatc
    21001 gaagagaacg tcgaccgcgc cgcgccgctc ggcgtcggag acgtaaaggt gggcggcaac
    21061 tatgccgccg ggctgcgcgc cagcctggac gccaagggca agggcttcgc cgatgccctt
    21121 ttcctggacg ccaaggagaa gaagtacata gacgagtgcg ctccggccaa ctttttcgcg
    21181 atcactaagg acgatcaata cgtgacgccg cacagcgagt cgatcctgcc ctcgatcacg
    21241 aacaagtcgc tgatcacgct ggccgaagac atgggcctgc gcccgcagcg ccgcccgatt
    21301 ccggtcgaag aaatattcga attcaaggag gccggctgct gcggtacggc ggccgtgatt
    21361 acgccggtgg gctcgatcac ttatcgcgat cgcaaggcgg tctactccaa agacggccag
    21421 cccggcaaaa tctgcacggc gctctacaac gagctaaccg cgattcagct cggcgatgcc
    21481 ccggatcgtt atcgctggct attcccggtg ccgcagacca agtgactcgc taacgacact
    21541 gcggctacgc cggcccgcac cgcaggctga cccgcccgca acgaccttcc cccaggcgcc
    21601 ggactttggt ccggcgcctt gtgttacgcg ctcttgcgca gttgctgaag atgcatggcg
    21661 tgggcgcggt ttgcatttcc tcgctgcggc catcgccgat tccacatgcc cgccatcaga
    21721 aatgcggcgc ttttttgccg acgcagcgtc tcgcgcggcc gagggctgat agactattga
    21781 agcggccgac gatgttccgc ggcccattcg tggcgcattg gcgccggcgg tgcattccgc
    21841 ccggccgcca tcggagaggt aaagatgagt ctcagagccc cttacgttat cgcactggcg
    21901 cttgtgctcc tggtgtccgg cttgatgggc tgcccgcgat tccccgagga aatcgaaatc
    21961 gagaagggcg acacgatcat cgtcgagggg ccgccgactt ttgcggacgc tgggtccgtg
    22021 ctcggtccaa ccgcgacgat catcgcggac gtctcgttga tcccgatcga ggtactgccc
    22081 gacgtcacgg tgctgacatt cgagaacctc accggcgtcg atattgtcat cagttacttc
    22141 gccgagggct tccccgaggc cgtgctcgta ccggatttca ccttcgtgag catcgagtac
    22201 gtgccctgtc tgctcgacgt gcaactcgac ttcgaggacg atttcgatcc ggtcacgggg
    22261 ctttacctgg gcagcttcga cctcagtgac atcctgctga tcgagggtct ggattatttc
    22321 tgcggagatg aaatcttgtt taccttcgat cccgacttcg tcttcgtgga cgtgttcccg
    22381 ctgtaaaatt cggggcacaa atagtacgcc gccattcact agcccctact tattgcactg
    22441 catctcccac tagcggaagc gaagactcgc ttttgccacg tgccgcgccc ccggcatagg
    22501 tagtacgcgc ctgagcattt cgggaagggt cgagttataa ttattctgtt agagtactga
    22561 tggggggcgg gcgcgcgcgt ggttcgcccg ggaggtgtta ggatggccaa tcgcccagct
    22621 caggtttgcc gctatcgcgc taccgttgag gcgctcgcgc acggcaaatt ggtcaacggg
    22681 cagcgcgcca ccgcgcagcg gcacttggag gagtgcgagc cctgccgcaa acttttccgc
    22741 gatcaaaacg ttgagtgttt tccgaccttc cgcaattaca ccatcgtgga gcgtgtgggc
    22801 gaaggtggtt tcggggtggt ataccgagcc gtttcgcaca ccaaacagcg caccgaggcg
    22861 ctgaaggtgc tttttggcaa gaccgccttg cgcgaggcct actttcagaa cgaagtgcac
    22921 ctggtcgcca aactacggca cccgaacatc gccaccctct tcgacgccca cctgactacg
    22981 ccccccctgt tctacaccat ggagttcatc tccgggcagc agctcggcga gtacttccgc
    23041 acccaccgcc ctcccctggc gcagcgcatc gccctgctgc agaaggtcgt tgccgccatc
    23101 agctacgctc attcccaggg cgtggtacac cgcgacatca aaccgcaaaa catcctggtc
    23161 gacgcgcgcg gcgaaccgat catcgtcgac ttcggcatcg gcaagaaact cggcctgccc
    23221 tgcaacgacc acgaaccaaa cggctcgcct gagggttttc tcggcacgtt cggctttgtc
    23281 gcccccgagc aagccgccgg tcagccggtc gatgagcgag ccgacatcta cgccctcggc
    23341 gctttgctat accattgcgt cactggtgaa ccggcccgtt cgggcaatga cgccgcccac
    23401 ctgacccaaa ttttccaaca gcgagagatc acgcgtccgg ccggcttggc cgccatcatc
    23461 gcccgctgcg tcgatcccac ccctgaggag cgctaccccg actgcgccgc actgctccaa
    23521 gacatcgccg cttatctcag cggcggccgt atccaggccg agaccaatac gaatccggcc
    23581 cggagcgtcg cgcgcgccgc ggcttatctg ctgcagcacc ggccgttgcc ggtgtgcggg
    23641 ttcctggtgc tgttgatggc aagcctgctg gcgacgttcg cctggcgggc tgatgcacgt
    23701 tgggccctgc cgggcagcag cgcacagacc gcggccctca tcactttcac gcccgatacc
    23761 atcgaggcta ttcgcaccgg ccagatcggc gctgacctgc cgggcctgaa tgttgaaaat
    23821 cgcaagagtc tgcgcctcct ccagggtcag ctcttggaac gcctggccgg cgcccagccc
    23881 cgcgtggtag tgatcgacgg gtattacccc gactgccagc cgcagttc
//
LOCUS       DAOSYS010000021        23851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1751_length_23851_cov_5.040847, whole genome shotgun sequence.
ACCESSION   DAOSYS010000021 DAOSYS010000000
VERSION     DAOSYS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23851
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1751_length_23851_cov_5.040847"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLU99_02390"
     CDS             <1..997
                     /locus_tag="PLU99_02390"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOO15933.1"
                     /translation="DYGDWLNADTLKLEGWPATGAAVPKEIFATMFFARSTQLVSQIA
                     AVLGRDDDARRYARLAADIRAAFLKAYVGPDGRMPGDTQAGYAIALHFDLLPEDLRPA
                     AVQHMLAGFEKYNGHISTGFHSTICMMNELNRLGLVDEAYRLLMNRGMPSWLYPVEHG
                     ATTIWERWDAYVEGRGFQDPGMNSFSHYAFGCVGEWMYRTILGINPDDPTGAEPGRTE
                     MPPTAEAWRPGYKHFTLRPRPGGGLTWAGGEYHSIHGKIVSHWRIENGRLAYECTIPA
                     NTTATVYVPTSDPASVREEIDPEALPPGSATRTEAGAAVFELGAGSYRFTCAYSL"
     gene            1066..2193
                     /locus_tag="PLU99_02395"
     CDS             1066..2193
                     /locus_tag="PLU99_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO15934.1"
                     /translation="MEPANTPSVDFTQLNLDVVFGRAGGRIIWQPRILAWLTDKQFAG
                     EPVPAPYTNMTPPQLYRALGCSNRIYDFNACFVSHEDPRVRLTRTELNATDYELIWET
                     PVGRQRAAYRRSPNAPAELPVKWPVSDEEELKVATWRELHRTWSWDQAAYERLSREWA
                     GLGAPTMYICRTTLQKLIIEDMGMTATVMAMRRFPAACEAYFEALAVTQERLIAVINA
                     SPIQIVNFGDNIHAMFLSPRWFRQYILPVYQRRCELLHAAGKFVHAHWDGHCKPLLPM
                     ARETGLDGIEAITPLPQGDVTLEEVKAALGDMFLLDGIPAVYFDHTYSEQELLDCTRK
                     CIDLFAPRLVLGISDEISSHGDIDRIRAVGRVVDEYNAGCG"
     gene            complement(2219..3442)
                     /locus_tag="PLU99_02400"
     CDS             complement(2219..3442)
                     /locus_tag="PLU99_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15935.1"
                     /translation="MSLPKSLEDEAQRVLPLVRAAFLSVLDSIPARPRRASEICRELG
                     ISQTLAWRIVQVADGGDPFAAVRYVPGESAVETFLGAAQAHGAPLEALQRARSAVAQF
                     KKLVRDHAGNRRSLELMMAGLARSGRAEADLPYRKEGFRCASHTWGIQVQTHIRTALL
                     YPGSRDDCVHIAHVHGYYNLRRVRPEARWVLARRYMMTEDGAAHRVPVSEPIAPEFAL
                     ENGFPLLRPFCSDPPPDIQRVPGPGNAIDDVWTKGAVGDQAAVTFYSGEVMRELPFRY
                     CDARNKYAQFGLHIRTAVETLVLDVLVHRDLYGPLSPECLVLSDLWSAPWETGGDVSQ
                     ADLLPCAEQVQFLGQGLAGVQTSEVPNYERLLHYVCEKLSWDGSQFHVYRLRMPYPVI
                     GTAVAMRFPLPERPR"
     gene            3775..6321
                     /locus_tag="PLU99_02405"
     CDS             3775..6321
                     /locus_tag="PLU99_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15936.1"
                     /translation="MQEKRHIIGPGIVFLCTGLIATSAALAQSRDVPANDLCENAFAV
                     TGPYPVTVCGTNVDATIDCPGFLDWDAVWYEVELPYAFNDLVTDYCPGPIDMSADTVG
                     IVYYNDCTDCEAAVVGAYQWNDCGNGRWHPKIWWLQLPGPATVRIPVYIAPAHEFCIE
                     FNVTESFPCDLDCPPEAVPESESCGELTNDGCNLPVPQFEPLTCGETICGTTFAYAGM
                     RDTDWFEITVEEPTTMTFTVEAEFIVGMFTGLFEQRTPGVAACDQITGLLIPYAIAAD
                     CESVSLVYNALPGTYWWLVTPNDYQSVPCHGFNQYIATMTCEPWELRGACCDDDTSLC
                     ADSVLFQDCLEPLRFTAETLCADISPPCGGCPESMIEIEIRTDRYPTETTWTITDHDT
                     GEVLYSGGPYSDDFATYIEYYCIGADDCVDFTIYDLCGDGIWPPYGYAVRLDGVTIYD
                     TIGIGWAGLERHNDNIGHGCELGRCCYEPWPNCAETTLFDCVRLYDGIWNEGLNCTDD
                     PCPDPNACDFEVVAPYTSPQRTTCGGLNRCQSPDHYFDTPEHVYCVTIPHDGIWSFNT
                     CLDTTNATWLAVGTEMCGQDIGWAAYTCWGFSAEVVAYLSAGTYFVDVEGYQQCGTYV
                     LDIHEVIPCDVNCPEEAVAESEPCGDTINDGCWMYPYAFERIVPNVPVCGTIWAADGS
                     YDTDWFEFVAPADDIMTFSVRSNFTALMGQAQQYVPGQPGCHNITGVIEPFAEVSDCE
                     QQSVSFPVVAGGTYYLYIGHRSSHNSPCGTTNDYVALLRGSSPVCGDLNCDGATNGFD
                     IDPFVLALVDPAGYAAAHPDCDYMRADLDGDGAVNVFDIDPFVLCLTTGG"
     gene            complement(6386..7504)
                     /locus_tag="PLU99_02410"
     CDS             complement(6386..7504)
                     /locus_tag="PLU99_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15937.1"
                     /translation="MAKPRRLVMPSLRTIWRSIISLVKRAYAAALIVLVLWLSFLAIR
                     YLVVTLMFPTPTPAQITGIPKRLTEAMLESRLTEWPGVISTERPRAPLARYHRLDQWI
                     QPDRFNSCAQSGCHSPLPHARRKEVRAFLNMHATALHCAVCHLKADQQPLPTVWYDLD
                     TGRARSAPDVLAAYGWLTSEAGRQELADPTPDTQRRLVELLRGAARQSGDVPALTQLA
                     EHVAAVHYSSPAFQQLVETARVTLPRHFRGEYGAKLTLLDTSSQQPVLESPGAEPAIR
                     AYLRESASGDAARLPELWAAIHANRRPQTLHCTDCHTAAGGLLDFAAAGYPAARLEEL
                     SRPGVFTMIEHIAGGQPFQLPGFARPEEPQQSQPAPRP"
     gene            complement(7497..9482)
                     /locus_tag="PLU99_02415"
     CDS             complement(7497..9482)
                     /locus_tag="PLU99_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOO15938.1"
                     /translation="MPLAGKVRKQRAGTALKNTRERCMGNGQERHGKNERLGWRATLS
                     AVRAAALILAGLVTLTPAASLAADPDNCLFCHQFRGLSRYDREAGGVHVYFVDPDYFH
                     DLAGPHARLDCTACHPREEVAVVPHLTVSRVNCTQVCHLSEPGRSERRFSHSNVAAML
                     DASVHTREMLSQIEFAGGPLLEPQQSVCLYCHDEPVFRDPANVFPALPALGSRAFDRC
                     DVCHREQIPVDAAYYLRHVASRLQPARPPLEQTQVCAVCHSDPEVRRTHDLTDAVASF
                     LRSFHGKAALLGDHSTANCLDCHVAEGENAHLMLGRADPRSSVNPANVASACRSTACH
                     PGADPQLAAASVHLDLPTARGTLEFLIAAAFILLTIGTFGPSLLLCVLELFQIVIGRH
                     HEGRREAQALVGAVMAHPEGRRRLVRITVSQRYQHWILVLLFVTLAITGFPMKFADRA
                     WARSVVDSLGGLHVTRTIHHWAGVALMLGFVAHLVYAFPALLRIARRPAPGGGRVGLL
                     RGVWQLPMVIAPWELPKAIQQLAYLVGLRREPPTFGRFSVKEKFEYIGVFWGTTLLGV
                     TGLLLWGQQFFSHYFSGRVLNIALIAHTYEAFLAIIHVGILHIVNVVFSPHVFPLSMA
                     TITGATPVTELADAHSEFVQDVARDLGIRGPEDVSRG"
     gene            10062..11279
                     /locus_tag="PLU99_02420"
     CDS             10062..11279
                     /locus_tag="PLU99_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOO15939.1"
                     /translation="MCHKNVHDDWAATLHAKALDNLEAIDQGSNPACLLCHTTGFGQE
                     GGFVSRNTTNALAGVGCETCHGPSRDHVENVADAALRPPVNISSDICSQCHNPFHHAL
                     FGEWQASLHAVVTEHVAEELVAGEAATSCGTCHSGDVRQRMFVQNEAVPDNMLEGRDI
                     ARLNAIECATCHDPHARTNRAFLPPEGHDFQLRYAEVVPFPPASNVVSETTDPARFNL
                     CGQCHHDRGTTWVSGGRPSHHSIQSNFYVGEMPLPPGGSVLVPNQRTVHAFAPKQCVT
                     CHMQREVEEDVVSAIHANHSFTVLDFTGCSSIGCHPSPEGAQADMAALQAEVQAGLDA
                     IAARLGDPATWEYTSAGGPDAAGQAAIPDVVKQVRFMYYWILNDGSLGVHNPEYARSI
                     ITQAGILLTSIGK"
     gene            complement(11328..12605)
                     /locus_tag="PLU99_02425"
     CDS             complement(11328..12605)
                     /locus_tag="PLU99_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15940.1"
                     /translation="MATVLALAGFSLVAGCPSAQPVPGPVFNNTTDPTNGGASFIGSA
                     ACSACHADIAELSSLHGHSFMLNPIAGAPPEYPVQAERAGVPDPPAGQTFDDVSYVIG
                     GYTHYALFVRTDGFLMTDGTEGVPTQWNLAFRANGTPAGFVSFMPGQTEPLPYAFDCF
                     RCHTVEPQPQTAASPLSQDNRPGILGTWAEPGVRCEACHGPGSNHVPRPSARDLFVDS
                     SAKACGRCHTEGDDPEVILAAGGFIVANTQFAELRASGGHANFACTVCHDPHASSTYD
                     PARGIRNACTVCHPDRGLAAHAGVTFTRGDYSEPVTCQSCHMPFATLTASVATAAIVG
                     EAGRMADTRTHIFRINVNPVPYTALFTADQTRVEKDEQGRAAVPLGFVCLRCHNDAAT
                     PNSAFPLGLDIASEIATDMHAKFDGGVLKDRTP"
     gene            complement(12662..14464)
                     /locus_tag="PLU99_02430"
     CDS             complement(12662..14464)
                     /locus_tag="PLU99_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO15941.1"
                     /translation="MDWAIFLFGAPPSPPLTIANPLGLAVDQDSMLICDGVLGALLRW
                     DLNTAQFSVQDFKPPLPYPFAIEVTPGGDWLVCDREGVHRVARGCVTGTYRPPQEPFR
                     PGGVLGVDGRVWVTNVVGHCIEIFDAESCQHLQSVFGADQPETQLAWPTALARMPDGN
                     VCVVDVLKNRVQVFSPEGQWLRSIGRPGDSVGEFGRPKHVAVGPDGAVFVTDAFSQRV
                     HVFTSDGQPLLAFGEPGSGIGALTLPAGIAISARPLPADVALPADAAPAYYVLVAEQL
                     NQPGIRVYAWLAGALEPAGGPLPLGEALTWQPSFPGSAAINPHWDPQRCTTCHTAAGG
                     QLMPIPPEETDALCLSCHDGVRAPAEPHPIGRKADTELVDTPDDWPAFGGVIGCLTCH
                     DILRHCHEAARRPAVNSVLLRNWDPQRPLEYCGNCHKVDVGRFSPHRQRDATGRVRED
                     ACLFCHTRHPEIPPDGRRTFQPHLRVETSELCLNCHTRHWDLSPLGHVDRPVSPKIRQ
                     WMLMRELGFKTDADVRELGRMAREMDRPPARLPLGQEREGTEIVTCYTCHNPHYAGLF
                     PPGSELGALAADRLDRASALRTDWIDLCSECHQR"
     gene            complement(14692..15387)
                     /locus_tag="PLU99_02435"
     CDS             complement(14692..15387)
                     /locus_tag="PLU99_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO15942.1"
                     /translation="MPPLVAIGLAALAAGSGFSLVRVLAAQPAPASQPQNHAELSPTE
                     PQEAAATQPAESVEPAGQRLVSGLIGSAHDFTRLGYGRDLCLPCHTPHQAFSEPPLLD
                     RRGVTPLPLRPYQAGEVVLTGWSLLCLGCHDGITASDVYSSPHATTVAAQINAARFGA
                     PGLRSHPVGVRQPDTAEKYYPPEAVEAAGLPLVDGRIQCTTCHDAHNTHGYSHMLRNS
                     NQGSRLCLTCHRL"
     gene            15590..17803
                     /locus_tag="PLU99_02440"
     CDS             15590..17803
                     /locus_tag="PLU99_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15943.1"
                     /translation="MSNAPVLLGVLLAVVGFQVGGAAAQQPATRPAGVETEPAPPGTQ
                     PAPLIAPETPEQPYEAPPTPDVCTSAQRLHFDELLFDLGFEAQADRRKVRSRVYDRYL
                     KREFQKKTTAGSLQETVDVYTGGWAFGERVLRFDVGARWGLSQNRFSEVSPAPDITNR
                     PHGDVLEYDLKFKLFPRGVVSATAFASQLDSRVPRPFLPSLDRTQERYGTSVFVNSPT
                     FPMRFSFEHLWENLTSRTRELNDEQQYGDDRFEYEGTWQISERQSLRLEYRHYDYTER
                     YSGTRTQFNTSGNYLLANHTLRFGPDDRSSLETLARLQDESGDLEQDIAEVSSRLRLQ
                     HTDSFATNYAVQFLRNAFHELQTETYRGEAGLTHQLGECLTSTLQFYGLKQDANKNAD
                     FTEWNTLANLAFSKPNPLGRFSANLSYNHTATDASDNNRRGIAIAESVTFRDPLPAFL
                     AQTDVDITTLVVTDSTRARVFLPVRDFIVLKLGRYTALQRVPTGMIQDRQTVLVTYTY
                     RVSSNYDVRRDRVDFRMQQEFKCGLTPYYAGSMQNEDVTQARFLRFEPRNVNRHRLGA
                     TYRRPRWSVGGEYEYNDDAIEPYQAVHFNGDVVLLQKLQHQLDGKATLSRFWFDDSYS
                     FSRWNDSGLTWAESVGAGRWTSTLLDLGAAYRYLVRRDLEASASAMYRYQHDSIFGDT
                     HGVDLSAAVEWRIGYFSLRFEAEYDVLDLPDSLDHGASFWIKLKREIPVIARTPQ"
     gene            17800..18849
                     /locus_tag="PLU99_02445"
     CDS             17800..18849
                     /locus_tag="PLU99_02445"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOO15944.1"
                     /translation="MRPVPAYTLFALFAAFAAFALCTGCQSPPRPIFPAISPDIVWPL
                     PPDRPRIKYIGELTGEASLGAKPRGMQALRQVLTGPPPTVAFVRPAAVAVLGERVFVA
                     DTGLALVHMLDLGERRYRAIQGNPADPLRIPIDLAIVDDATLVVADRSRAALDIFDRD
                     GNWQATRRFPEITAPAAIAYDSFSRRLWVADSATHCCLAVTALERVTARIGQRGSEPR
                     QFNFPTALATHPAVGLVVADAMNFRVQVFNASAEPVAVFGQKGDAAGDFARPRDVALD
                     SEGHIYVLDNQFENVQIFDRGGRLLMAFGSEGTGPGEFSLPSGITIDAQDRIWIADSL
                     NRRVQVFQYLAEGAS"
     gene            18846..20552
                     /locus_tag="PLU99_02450"
     CDS             18846..20552
                     /locus_tag="PLU99_02450"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO15945.1"
                     /translation="MIASWSAKRMIVLAVLAIAARADERIANSKHDLSAMGPGPTRAV
                     NESEICIFCHTPHNASPVAPLWNRYNPTTYYRIYRSRTLQARVDQPGPASKLCLSCHD
                     GTVALGLVLNRPFTNPIPMNHPYITGPADLTTDLSDDHPIGFRYDQQLANVDPQIREP
                     GLVDFRIKLGERGELECTACHDPHNNELGNFLRITQREGALCRTCHDMRGWEHSAHAL
                     SPRNVPLTVTQGERLPYNSLAENACLSCHVSHSAPWREQLLRDRPSRLCLDCHNGVAA
                     VDIAPVINQLSGHRMNPVWESRERGELGFRISPFTQCTACHNPHAVRLNIPGEALSVA
                     LNTPFVPPAMRDVPGVNLAGVPVPVATFYYEVCFRCHADFPVLVRDRIVRQIDTLGNV
                     RRQFLPTVASAHPVTFPSRRSTDVPSLAPEFRTRLFISCQDCHNNPNAYDAGGGLASG
                     PHTSRYNFLLVAQYETQDFTMESPQAYALCYRCHDRTSILNNESFPLHRVHIVNGRSP
                     CSACHTAHGVGGSTPQHDHLINFDISIVGGQRFYNDTGRFSGTCTLTCHGVQHVNFTY
                     AP"
     gene            complement(21181..21768)
                     /locus_tag="PLU99_02455"
     CDS             complement(21181..21768)
                     /locus_tag="PLU99_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15946.1"
                     /translation="MASFRGSEEGLIGPRTRPQGVFDGLGNGVQWTFDDGNRRESAAL
                     SNGAASMFAYDLNNRLLQIQHGDVGAALDPFYDVAYGYDPVGNRLFKQDFERPNRNEV
                     YSYDQRHRLTLDTAPAASRPGSRSDPRASHATPCQGLTRFRRGTIAFDGEGAAGIVTP
                     INDPQMPSFRLPGPTRPTRRAQARREHSRTACRSR"
     gene            complement(22488..>23851)
                     /locus_tag="PLU99_02460"
     CDS             complement(22488..>23851)
                     /locus_tag="PLU99_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOO15947.1"
                     /translation="VAEIDGNPLLEIEYDALGRRIASTEYGVAVDPCTGDAGPVTVQT
                     RHIYSGLNAVAEYISCDGGDWGLAREVLWGERFPEPLALFDFTAAGDVEAGTEEVLHY
                     VHDALGSVVGLVDAGDPDATPEPVPAKLVERYDYDPYGKTYIETWEAIGSGGAGAWMR
                     ARTSAYGNPFTWTGQRYDASVGMYHFPFRSFSPELGRWMQRDPLGYVNGISLYAYVLG
                     NPLYWTDPLGLKIWWQPGRGSTPDDMDKAQRQLADARTRRNPDGSMGRGAQDIERLNQ
                     DEEGGVVVVVGPNDGNVAMKISNRVAVISHDPNREETYDDGVKRDPESALVHEMRHAA
                     RGIYGPKPKSERESETDAGSVENEHRRNKGEEQRRKYGDWPLEQHDVMPPVVAAPNPW
                     LPLLLQAYHDAWLAWWRWRNFVGPLTPSERQEADEAYDEARRLDDLVKKWAPEALKPV
                     ICW"
BASE COUNT         4435 a         7975 c         7271 g         4170 t
ORIGIN      
        1 cgactacggc gactggctca acgcggacac gctcaagctg gagggctggc cggccacggg
       61 ggccgcagtg ccgaaagaaa tcttcgccac catgttcttc gcacgctcca cccagctcgt
      121 ctcgcaaata gcagccgtac ttggccgcga cgacgacgcc cgccgctatg cccgactggc
      181 ggccgacatt cgcgccgcct tcctgaaggc gtacgtcggc cccgacggcc gcatgcccgg
      241 cgacacccag gccggatacg ccatcgcgct gcacttcgac ctcctgccgg aagacctgcg
      301 gcccgcggcc gtgcagcaca tgctggccgg ctttgaaaaa tacaatggcc acatctccac
      361 cggtttccac agcaccatat gcatgatgaa tgaactgaat cgcctcggcc tggtggatga
      421 agcctaccgg ctgctgatga accgcggcat gccctcctgg ctgtacccgg tcgagcacgg
      481 cgcgaccacc atctgggagc gctgggacgc ctacgtcgag ggccgcggct tccaggaccc
      541 cggcatgaac tccttttcgc actacgcttt cggctgcgtc ggcgagtgga tgtaccgcac
      601 tatcctcggt atcaatccgg acgacccgac cggagccgag ccaggccgca ccgaaatgcc
      661 gccaactgcc gaagcgtggc gcccaggcta caagcacttc acgctgcgcc cgcgacccgg
      721 cggcggcctg acctgggccg gcggcgagta ccactcgatc cacggcaaaa tcgtgagcca
      781 ctggcgcatc gaaaacggcc ggcttgcgta cgaatgcacg atcccagcca ataccactgc
      841 gacggtctac gtcccgactt cggaccccgc gtcagtacgc gaggagatcg accccgaggc
      901 actaccgcca ggctctgcga cgcgaacgga agccggcgct gcggtattcg aactcggcgc
      961 gggtagttat cgtttcactt gcgcgtactc actatgatgt ttgcgtgctt tgtggtggcc
     1021 ttgtagctaa atgctccgcg ctgtgtcttt ggtgcagaga atccaatgga accagcaaac
     1081 acaccgtcag tcgacttcac gcaactgaac ctcgacgtgg tcttcggccg cgccggcggc
     1141 cgtataattt ggcagccgcg catcctcgcc tggctgactg acaagcagtt cgcgggcgag
     1201 cccgtgccgg ccccctacac caacatgacc ccgccgcagc tctatcgcgc gctgggctgc
     1261 tccaaccgca tctatgattt caatgcgtgc ttcgtttcac acgaggaccc gcgcgtgcgc
     1321 ctgactcgca cggaactgaa cgcgaccgac tacgagctca tctgggaaac gccggtcggc
     1381 cggcagcgcg ccgcctatcg ccgctcgccc aacgcgcccg cggaactccc ggtcaaatgg
     1441 cccgtgagcg acgaagagga acttaaagtc gccacctggc gcgagctgca ccgcacctgg
     1501 tcctgggacc aggccgccta cgagcgcctc agccgcgagt gggccggcct cggcgccccg
     1561 accatgtaca tctgccgcac cacgctgcag aagctcatca tcgaagacat gggcatgacc
     1621 gccaccgtga tggccatgcg gcgcttcccc gccgcgtgcg aagcttactt cgaagccctg
     1681 gccgtgacgc aggaacgcct catcgccgtc atcaatgcca gcccgatcca gatcgtcaat
     1741 ttcggcgaca acatccacgc catgttcctc tccccacgct ggttccggca atacatcctg
     1801 ccggtatacc aacggcgttg cgaactgctg cacgcggccg gcaaattcgt ccacgcccac
     1861 tgggacgggc actgcaagcc cctcttgccg atggcccgcg aaaccggcct ggacggcatc
     1921 gaggccataa cgcccctccc gcagggcgac gtaacgctcg aggaagtcaa ggccgccttg
     1981 ggcgacatgt tcctgctcga cggcatcccg gcggtctact tcgatcacac ttacagcgaa
     2041 caggaactgc tggactgcac gcgcaagtgc atcgaccttt tcgccccgcg tctggtgctg
     2101 ggcatcagcg acgaaatctc ctcccacggc gacattgacc gcatccgcgc cgtcggtcgg
     2161 gtcgtcgacg agtacaacgc cggctgcggg taacatttgg gctatctttt agcttgcctc
     2221 accgcggcct ttcaggcagc ggaaagcgca tcgctaccgc cgtgccgatg accgggtaag
     2281 gcatccgaag gcggtacacg tggaattgcg agccgtccca gctcagtttc tcgcatacat
     2341 agtgcagaag ccgctcgtag ttcggcacct ccgaggtctg cacgccggcc aagccctggc
     2401 ccaggaattg aacctgctcc gcacacggca gcaggtcagc ttggctcaca tctcccccag
     2461 tttcccacgg agccgaccag agatcgctca gcaccaaaca ttccggcgac agcggcccgt
     2521 acagatcgcg gtgcacgagc acatccagca ccagcgtctc caccgccgtg cgaatgtgca
     2581 agccgaactg cgcgtacttg ttgcgcgcgt cgcaatagcg gaacggcagc tcgcgcatga
     2641 cctcgcccga gtagaacgtc accgcagcct ggtcgccgac cgcccccttc gtccacacat
     2701 cgtcgatcgc attccctgga ccaggaacgc gctgaatgtc cggcggagga tccgaacaga
     2761 acggccgcag cagcggaaag ccattctcca gggcaaactc cggggcgatc ggctcggaaa
     2821 cggggacgcg atgtgccgcg ccatcctccg tcatcatgta acgacgggcc agaacccaac
     2881 gggcctccgg gcgcacccgg cgcaagttgt agtagccatg cacgtgcgcg atgtgcacac
     2941 agtcgtcgcg cgatcccgga tacagaagcg ccgtgcgaat atgcgtctga acctggattc
     3001 cccacgtatg gctcgcgcag cggaacccct ccttgcgata cggcagatcc gcctcggcgc
     3061 gcccactgcg tgccaggccg gccatcatca attcaagcga ccggcggttg ccggcatggt
     3121 cccgcacgag tttcttaaac tgcgccacag cagagcgagc gcgctgcaac gcttccagcg
     3181 gagcgccgtg ggcctgcgcg gcccccagaa aggtctccac cgccgactcg ccgggtacgt
     3241 agcgcacggc cgcaaacggg tcacccccgt ccgcaacctg cacgatgcgc caggctaacg
     3301 tctggctgat tccgagctcg cgacagattt cgctcgcccg ccgcggccgc gccggtatcg
     3361 agtcgagaac ggagagaaac gccgcgcgca ccaggggcag tacccgctgt gcttcgtctt
     3421 cgagtgattt tggcaggctc atactagctt ctctggggct aataccggct tcgttgggct
     3481 ctattcggcc ttttgggctc cactgagcgt ccccggaacc gcttccgggc cgtggccaca
     3541 gtttgctcta cgtaaacatt agttattaca cccctactct tgaaaagatt caagggtaat
     3601 ttcatgaagt ttttcaagaa agctcttgca tggccaatcc gatatcggta acatgcgtcc
     3661 ggagtgaggc gctatctgcg cgtttatcca gagcttccca ctctgggaat ctcgaacccg
     3721 cggagacgcc gttttttcgg accctaaggc tgaggaacac atagaggtgc gaaaatgcaa
     3781 gaaaagaggc acattattgg acctggcatt gttttcctgt gcaccggcct gatcgccacc
     3841 agcgctgcac tggcgcaatc gcgcgacgtg ccggccaacg acctttgtga gaacgcgttc
     3901 gccgtgacgg gcccctaccc cgtcacggtt tgcggcacaa acgtggacgc gacgatcgac
     3961 tgccccggtt tccttgactg ggacgccgtg tggtatgagg tcgagctgcc gtacgcgttc
     4021 aacgacctcg tgaccgacta ctgccccggc ccgatcgaca tgtcggcgga tacggtcgga
     4081 atcgtctact acaacgactg taccgattgc gaagccgcag tcgtcggcgc ctaccagtgg
     4141 aacgactgtg ggaacggcag gtggcacccg aagatctggt ggcttcagtt gcccggtccg
     4201 gccacggtgc ggattccggt ctacatcgct ccggcgcacg aattctgtat cgagttcaac
     4261 gtcacggaga gcttcccgtg cgacctcgac tgcccgccgg aagcggtgcc ggaaagcgaa
     4321 agctgcggcg agctgactaa cgatggctgc aacctgcctg tgccgcagtt tgagccgctg
     4381 acctgcggcg agacgatttg cggcacgact ttcgcgtatg ccggaatgcg cgacaccgac
     4441 tggttcgaga tcacagtcga agagccgacc accatgacgt tcaccgtcga ggccgagttc
     4501 atcgtcggca tgttcaccgg ccttttcgag cagcgcacgc ccggcgtggc cgcctgcgat
     4561 cagataaccg ggttgctcat tccgtacgcc atcgccgccg actgcgaatc ggtcagcctg
     4621 gtttacaacg cactgccggg cacgtactgg tggcttgtaa cgcccaacga ttaccagagc
     4681 gtcccctgtc acggcttcaa ccagtacatc gccacgatga cttgcgagcc gtgggaactc
     4741 cgcggcgcct gctgtgatga cgacaccagc ctttgcgcgg acagcgtgct gtttcaggat
     4801 tgcctcgaac cgctgcgctt caccgcggaa acgttgtgcg cggacatcag tccgccttgc
     4861 ggcggttgcc ccgaaagcat gatcgaaatc gagatcagga ccgaccgcta cccgactgaa
     4921 acgacgtgga ccatcacgga tcacgatacc ggcgaggtgc tctactccgg cggtccgtac
     4981 agcgacgact tcgcgaccta catcgagtat tactgcatcg gcgccgacga ttgcgtcgac
     5041 ttcaccatct acgacctctg cggcgacggc atctggccgc cctacgggta tgccgtccgc
     5101 ctcgatggcg tcaccattta cgacaccatt ggcatcggct gggccggcct cgaacgccac
     5161 aacgacaaca tcggccacgg ctgcgaactc ggccgttgct gctacgagcc ctggcctaac
     5221 tgcgccgaaa ccacgctgtt cgactgtgtc cgcctttatg acggcatatg gaacgaaggc
     5281 ctgaactgca ctgatgaccc gtgtccggac cccaacgcct gcgacttcga agtcgtcgcc
     5341 ccatatacgt cgccgcagcg caccacctgc ggcgggctca atcgctgcca atcgccggac
     5401 cactatttcg acacgcccga gcacgtgtac tgcgtcacga ttccgcacga cggcatctgg
     5461 agcttcaata cctgcctgga taccaccaat gccacctggc tggccgtcgg cacggagatg
     5521 tgtggccagg acatcggctg ggccgcctat acctgttggg ggttttccgc tgaggtcgtc
     5581 gcatacctca gcgccggcac gtacttcgtc gacgtcgagg gatatcaaca gtgcggtacg
     5641 tatgtgctcg atatccacga ggtgattccc tgcgacgtga attgcccgga agaagccgtg
     5701 gctgagtccg agccctgcgg cgacaccatt aacgacggct gctggatgta cccatatgcg
     5761 ttcgagcgga tcgtccccaa tgtgcccgtt tgtggcacta tctgggcggc cgatggcagc
     5821 tacgacaccg actggttcga gttcgtggcg ccggccgacg acatcatgac cttctcggtg
     5881 cggtcgaact tcaccgcatt gatgggccag gcgcagcagt atgtgcctgg tcagcccggt
     5941 tgtcacaaca ttaccggcgt gatcgaaccg ttcgcggagg tgtcggattg cgagcaacag
     6001 agcgtgtctt tcccggtcgt ggccggtggc acctattacc tgtacatagg acaccgctcg
     6061 tctcacaaca gtccgtgcgg gacgaccaat gactacgtcg cgctgctccg cggatcaagc
     6121 ccagtgtgcg gcgacctgaa ctgcgacggc gcgactaacg ggttcgatat cgatccattt
     6181 gtgctggcct tggtcgaccc ggccgggtac gcagccgcgc accccgattg cgattacatg
     6241 cgggcggacc ttgacggcga cggcgctgtg aacgtcttcg acatcgaccc gttcgtgctc
     6301 tgcctgacga ctggcggttg accggaccgc ccttaagtcc gggcggttgg gactgaaatc
     6361 aaatgcccaa taccgccctc gcacactacg gccgcggtgc gggctgcgac tgctgaggct
     6421 cctccggccg cgcgaaaccc ggcagttgaa atggctgccc gcccgcgatg tgttcgatca
     6481 tagtgaagac gcccggccgc gacagctcct cgaggcgcgc ggccgggtaa cccgcagccg
     6541 cgaaatccag caggccgccc gcggccgtgt gacagtcagt acagtgcagc gtttggggac
     6601 ggcggttcgc atgtattgcc gcccacaatt cgggcaggcg tgccgcgtcg ccgcttgccg
     6661 attcgcgcaa gtacgctcgg atggccggtt ctgcaccggg actctccagc accggctgct
     6721 gcgaactcgt atccaacagc gtcagcttgg ccccgtattc gccgcggaag tgccgcggca
     6781 gtgtaacgcg cgccgtctcc accaactgct gaaaggccgg gctgctgtag tgtacggccg
     6841 ccacgtgttc ggccagttgc gtcagtgcgg gcacgtcgcc ggactggcgg gcggcgccgc
     6901 gcagcaattc caccaggcgc cgttgtgtgt cgggcgtggg atcggccagt tcctggcggc
     6961 cggcctcgga agtcagccaa ccgtaagccg ccagcacgtc gggcgccgag cgtgcgcggc
     7021 cggtgtccag gtcgtaccac accgtcggca atggctgttg gtctgctttc aggtggcaga
     7081 cggcacaatg caaggcggtg gcgtgcatgt tcagaaacgc gcgaacctcc ttgcgccgcg
     7141 cgtgcgggag cgggctgtga cagccgctct gcgcgcatga gttgaagcgg tccggctgga
     7201 tccactggtc gaggcgatga tagcgcgcca gaggggcgcg cgggcgttcg gtgctgatta
     7261 cgcccggcca ttcggtcagc cgcgactcca gcatggcctc ggtcagccgc ttcgggattc
     7321 cagtgatctg ggccggcgtc ggcgtgggaa acatgagcgt gaccaccagg tagcgaatgg
     7381 ccagaaaact gagccacagc accagcacga tcaacgcggc ggcataggcg cgcttgacca
     7441 ggctgatgat gctgcgccag atggtccgca gcgacggcat gacgagccgg cgaggtttag
     7501 ccacgggaca cgtcctctgg ccccctgatt cccagatcgc gtgctacgtc ctgaacaaac
     7561 tcgctatgtg cgtcagccag ttcggtcacg ggggtggcgc cggtgatcgt cgccatggac
     7621 aacgggaaca cgtgcggcga gaagaccaca ttcacgatgt gcagaatgcc gacgtggatg
     7681 atcgccagga acgcctcata cgtatgggca atgagggcaa tgttcagcac gcgcccgctg
     7741 aagtaatgtg aaaagaactg ctgcccccac agcagcagcc cggtcacacc cagcagcgtt
     7801 gtaccccaga atacgccgat gtactcgaac ttctccttca cgctgaaacg cccgaatgtg
     7861 ggcggctcgc gccgcaatcc caccaggtag gccaattgct gtattgcctt cggcagctcc
     7921 cagggcgcga tcaccatcgg cagttgccac acgccgcgca gcagcccgac ccgccccccg
     7981 cctggcgccg gccgccgcgc gatgcgcagc agcgctggga aggcgtagac caggtgcgcc
     8041 acaaagccca gcatcagcgc cacgcccgcc caatggtgga tcgtgcgcgt cacgtgcaag
     8101 ccgcccagcg aatccaccac gctgcgcgcc caggcccggt cggcgaactt catcggaaag
     8161 ccggttatcg ccagcgtcac gaacaacagc accaggatcc aatgttgata ccgctggctg
     8221 accgtgatgc gcaccagtcg ccgccgccct tccggatggg ccatcaccgc cccgaccagc
     8281 gcctgcgctt cgcgccgccc ttcgtgatgc cggccgatca cgatctgaaa aagctccagc
     8341 acgcacagca gcaaagatgg cccgaacgtc ccgatcgtca gcaggatgaa cgcggccgcg
     8401 atcaggaact ccaaggtgcc gcgcgccgta ggcaaatcca ggtgcacgct ggccgccgca
     8461 agctgcggat cagcgccggg atgacaggcc gtgctgcggc aggcgctggc cacgttcgcc
     8521 ggattcaccg aggagcgcgg gtcggcccgc cccagcatca ggtgcgcgtt ctcgccttcg
     8581 gccacgtggc agtccaggca gttggccgtg ctatggtccc ccagaagcgc ggccttgccg
     8641 tggaagctgc gcaagaagct ggcgacggcg tccgtcaggt cgtgagtgcg gcgcacctcc
     8701 ggatcgctgt gacagaccgc acaaacctgc gtttgctcca gcggcggccg cgctggctgc
     8761 aagcgcgaag ccacgtgccg caagtagtac gccgcgtcga ccggaatctg ctcccggtga
     8821 cacacgtcac agcggtcgaa cgcccgcgag cccagcgccg gcagcgccgg gaagacgttg
     8881 gcggggtcgc gaaacaccgg ctcgtcgtga caatacaggc agacagactg ctgcggttcc
     8941 agcaacggcc caccggcaaa ctcgatttgg gacaacatct cccgcgtgtg gacgctcgcg
     9001 tccaacatcg ccgccacgtt gctgtggctg aagcgccgct ccgagcggcc cggctcgctc
     9061 aagtgacaca cttgcgtgca gttcacgcgc gataccgtaa ggtgcgggac caccgcaacc
     9121 tcctcgcgcg gatggcaggc ggtgcaatcc aggcgcgcgt gcggcccggc taagtcgtga
     9181 aaatagtcgg gatcgacgaa ataaacgtgt accccgccgg cctcgcggtc atagcggctc
     9241 aggccccgaa actggtggca gaacaggcag ttgtccgggt cagccgccaa gctggcagcc
     9301 ggcgtcagcg taacgagccc ggccagaatc agcgctgccg cacgcaccgc cgacagcgtc
     9361 gcccgccaac caagccgctc gttctttccg tgcctttcct gcccgttgcc catgcacctt
     9421 tcccgcgtgt tcttcagcgc cgttcctgca cgctgcttgc gcacctttcc tgcaagcggc
     9481 aatcgccctg ctgcagaact tcggcccgcg gcgaattgta gcggcaatcc ggccgtcacg
     9541 cagggtgcca tacccaagca gtaagcgcat gggtgccgtg cccaagctgt aagcgcgggc
     9601 atgcccctac caaaacccct aaccttgccg gacaacctgc cacggcctcc gcaaggtcgg
     9661 cacgcacttt tcgctacgtt ccagtgccgc cacctactac ggtttttatc agacatgccc
     9721 ggccacccag cttggtcatc gctcgggata caactacacc gaccaagata agccgccaaa
     9781 aaagatgttg actttgaaag tggatgccgt acgctgtcac ccacgttttc gaggttacga
     9841 acgcgctgac gggccatgcg ctgtcgtcac ctctttcctg tgcatcgaag ttgtctcgag
     9901 gagagaagac aatcatgtcg cgcagaacaa taacccgagc cggactagca tcgttactgg
     9961 gctgtgtcgt acttctgtct ggctgcccgc aaccgacacc agatgacaca cccggaaaca
    10021 gtggccttac gggcaagtac gtgggttccg aaacctgctc attgtgtcac aagaatgtcc
    10081 atgatgattg ggcggccact ttgcacgcca aggccctgga caacctggaa gcaatcgacc
    10141 agggcagtaa cccggcctgc ctgctgtgcc atactaccgg ctttgggcag gaaggcggct
    10201 tcgtcagtcg caacaccaca aacgcgctgg ccggcgtcgg atgcgagact tgccacgggc
    10261 cctcgcgcga ccacgtggaa aatgtggccg acgcagccct gcggccgccg gtgaacatct
    10321 cctccgacat ctgctcccag tgccataatc cgttccatca cgcgctcttc ggggagtggc
    10381 aggccagcct gcacgcggtg gtgactgagc acgtggcgga agaactcgtt gccggtgagg
    10441 ccgccaccag ttgcggcaca tgccactcag gcgacgtgcg gcagcggatg ttcgttcaga
    10501 atgaagccgt tcccgacaac atgctggagg gcagggatat cgcccgactg aacgcgatcg
    10561 agtgcgcgac ttgccatgat ccgcacgcgc gaaccaatcg tgcgttcctg ccgcccgaag
    10621 gacacgactt ccaactgcgt tatgctgaag tggttccgtt cccgcccgcc tcgaacgtcg
    10681 tttccgaaac gaccgatccg gcccgcttca acctgtgcgg ccaatgccac cacgaccgcg
    10741 gcaccacctg ggtatccggc ggccgcccgt cacaccatag cattcagtcg aatttctacg
    10801 tgggcgagat gccgctgcca ccgggtgggt ccgtgctggt ccccaaccag cgcaccgtgc
    10861 acgcgttcgc gcccaagcag tgcgtgactt gccatatgca gcgcgaggtc gaggaggacg
    10921 tggtttcggc catccacgcc aaccacagct tcaccgtgct ggacttcacc ggctgcagct
    10981 cgatcggttg ccacccctcg cctgaaggcg cgcaagccga catggcggcc ttgcaagccg
    11041 aggtgcaggc gggcctggat gccattgcag cgcgtctcgg cgacccggcc acctgggagt
    11101 acacctccgc aggcggcccg gacgccgcgg gtcaagcggc catccccgac gtggtgaagc
    11161 aagtgcgctt tatgtactac tggattctaa atgacggcag cctgggcgtt cacaacccgg
    11221 agtatgctcg ctcgataatc acgcaggccg gcatcctgct gacaagcatc ggcaagtagc
    11281 cgggcgcgac agcgcgggaa aattcgttga gcggccgcgg caggccgtca gggagtgcgg
    11341 tctttcaaga cccctccatc gaacttcgcg tgcatgtcag tcgcgatctc gctggcgata
    11401 tccaggccga gcgggaacgc gctgttaggc gtggctgcgt cattgtggca gcgcaggcac
    11461 acgaagccca gtggaaccgc cgcgcgtccc tgctcatcct tctccacacg agtctggtcg
    11521 gccgtgaaca gcgcggtgta cggcaccggg ttcacattta tgcggaaaat atgcgtgcgg
    11581 gtgtctgcca tgcgccccgc ctcgcccaca atggccgcgg tcgccactga agcggtcagg
    11641 gtggcgaagg gcatgtggca gctttggcag gtaaccggtt cgctgtaatc gccgcgggtg
    11701 aaagtcactc cggcgtgggc cgccagaccg cggtcgggat ggcaaacggt gcaggcattg
    11761 cgaatcccgc gagccggatc gtaggtactc gaggcgtgcg ggtcgtggca aacggtgcac
    11821 gcgaaattgg cgtgcccgcc gctggcgcgc aactcggcaa actgcgtgtt cgcaacgata
    11881 aatccgccag ccgcaagaat gacttcggga tcgtcgccct cggtgtggca gcggccgcag
    11941 gccttggccg acgagtcgac gaacaggtcc cgtgctgacg gtcgcggaac gtgattggag
    12001 ccgggcccat ggcaagcttc gcagcgcacg cccggctcgg cccacgttcc gagaattccg
    12061 ggccgattgt cttgtgacag gggcgaggcg gcggtctgtg gctgcggttc gacggtatgg
    12121 caacggaagc agtcaaacgc gtatggcagc ggttcggttt ggccgggcat gaatgagacg
    12181 aacccggccg gcgttccgtt ggcccgaaac gcgagattcc actgggttgg gacgccctcc
    12241 gtaccgtccg tcattaggaa gccatcggtc cggacaaata gcgcataatg ggtgtagccg
    12301 ccgattacat aactcacgtc atcgaaagtc tgcccggccg gcggatccgg aacaccggcc
    12361 cgctccgcct ggactggata ctccggcggc gcgccggcga tcggattcag catgaaactg
    12421 tggccgtgca agctgctcaa ctcggcgata tcggcatggc aggcgctgca ggcggccgag
    12481 ccgatgaagc tggcaccgcc gttggtggga tcggtagtgt tgttgaacac cgggcccggg
    12541 acgggctggg ccgacgggca gccggcaacc agcgagaaac ccgctagggc gagtacagtt
    12601 gccaacagtg cggctcgccg ggagccgcgc cctcccgtga cgtgcctcgg actaagatgg
    12661 cctagcgttg gtggcattcg gaacataggt caatccagtc tgtacgaagc gcgctggcgc
    12721 gatccagtcg gtcggcggcc agcgctccca gctcactgcc gggcggaaac aggccggcat
    12781 agtgcggatt gtggcacgtg tagcaggtga cgatttccgt cccttcccgt tcctgtccca
    12841 atggtaatcg cgccggcgga cgatccatct cgcgcgccat ccgccccagt tcccgcacgt
    12901 ccgcgtcagt cttgaaaccc agttcccgca tcagcatcca ctgtcggatc ttcggcgaca
    12961 ccggccggtc aacgtgcccc agcggcgaca aatcccaatg ccgcgtgtga caatttaggc
    13021 acagctctga ggtctcaact cgcagatggg gttggaacgt acgccgtcca tcgggcggaa
    13081 tctcgggatg gcgtgtgtgg caaaagaggc acgcgtcttc acgcacacgg cctgtggcgt
    13141 cgcgctgacg gtgcgggctg aagcgcccaa cgtcgacctt atggcaattg ccgcaatatt
    13201 ccagcggccg ctgcgggtcc caattacgca gcagcactga gttgactgcc gggcgtctgg
    13261 cggcctcgtg gcagtgccgc aaaatgtcgt ggcacgtgag acacccgatc acaccgccga
    13321 acgccggcca gtcgtccggc gtgtccacca actcggtgtc agcttttcga ccgataggat
    13381 gcggctcagc gggtgctctg acaccgtcgt ggcaggacaa gcacagggca tccgtttcct
    13441 ccggcggaat cggcatgagc tgcccgcccg ccgcagtgtg gcaagtcgtg cagcgttgcg
    13501 ggtcccagtg cggattgatt gccgcggagc ccggaaagct gggttgccaa gtcagggcct
    13561 cgcccagcgg gagtgggccg cccgcgggtt ctaaagcgcc ggcgagccag gcatagactc
    13621 gtattccggg ctgattcagt tgttcggcca cgagcacgta gtaagccggc gcggcgtccg
    13681 ccggcaaggc tacatcggct ggcaacggcc tcgcggaaat cgcgattcca gccggcagcg
    13741 tcaaggcgcc gattcccgaa cccggttcgc caaacgccag cagcggctga ccgtcggacg
    13801 taaagacgtg cacacgctgc gaaaacgcat cggtaacgaa caccgcaccg tccgggccaa
    13861 ccgccacgtg cttggggcgg ccgaattcgc cgaccgaatc accaggccga ccaatgctgc
    13921 gcagccactg cccctcgggg ctgaagactt gaacgcggtt tttcaacacg tcgactacgc
    13981 acacgttgcc gtccggcata cgcgccaagg ctgtcggcca tgccagttgc gtctccggct
    14041 ggtcggcgcc gaaaacggac tgcagatgct ggcaggattc ggcgtcaaag atttcgatgc
    14101 agtgtccgac gacgttcgtg acccacacac ggccgtcgac gcccaacacg ccgcccggcc
    14161 tgaatggttc ctgcggcggc cggtatgtgc cggtgacgca gccgcgtgct acgcggtgaa
    14221 cgccctcgcg gtcgcaaacg agccagtcgc cgccgggcgt gacctcgatt gcgaacggat
    14281 acggcagcgg cggcttgaaa tcctgcaccg agaactgagc cgtattaaga tcccagcgca
    14341 ggagcgcccc cagtacgccg tcgcaaatca gcatcgaatc ctgatcgacc gccagcccca
    14401 gcggattggc gatggtcagc ggcggcgaag gcggcgcgcc gaaaagaaaa atcgcccagt
    14461 ccacttccgc cggcgatgga ggaggcccgc cacgaaaagt acctagcgct attacgcgcg
    14521 gcggctgcgg cgccggcgga tagaaccgct cgattgcccg cggcgaccct ggtcggcccg
    14581 cttggcaccc gaggccaaga aaccccaata aactaccacc tgcccccagc acaagcacta
    14641 tccgcagcgc ccgccatcca cgccggcgct gacccggaca gcgaggccgg cctagaggcg
    14701 atggcacgtc agacacaggc ggctaccttg attcgaattc cgcaacatgt ggctgtagcc
    14761 atgagtgttg tgggcatcgt ggcacgtcgt gcattgaatg cgtccatcaa ccaacggcaa
    14821 accggccgcc tccaccgctt caggcgggta gtacttctcg gcagtgtccg gctgccgaac
    14881 gcccaccggg tggctgcgca accccggggc gccgaaacgg gccgcgttaa tctgggccgc
    14941 gaccgtcgtg gcgtggggcg acgaatatac gtcagacgcc gtaatcccat cgtgacaacc
    15001 gaggcacaac agggaccagc cggttaagac cacctccccg gcctgatagg ggcgaagcgg
    15061 caagggggtg acaccacgcc gatcgagaag cggcggttcg ctgaaggcct gatgcggcgt
    15121 gtgacatggc aggcacaggt cgcgcccgta acccaggcgt gtgaagtcat gtgcgctgcc
    15181 aatcagcccg ctcaccagcc gctggccagc cggttccaca ctctccgccg gctgagtcgc
    15241 ggcggcctct tgcggctctg tgggcgataa ttcggcgtgg ttctgcggct gactggcggg
    15301 cgccggttgg gccgccagaa cccgtaccag cgagaaccca ctgccggcag cgagcgccgc
    15361 caacccgatc gccacaagcg gcggcattcg atgcaaacgc ccgtggatgg acttgcctct
    15421 cacggcttgg ccctttcgtg tgactggtga ggactatacc ggccgcggcg gtcagccaca
    15481 acagacccgc gatattgcgg cgcgcgctgg acagtcgctt tggcgagggg tagcttgcca
    15541 gcgcgcttgc ccagcggtcg gcaggcgcga cctggccaaa accgagagta tgtcaaacgc
    15601 acccgttctt ctcggcgttt tgctggccgt ggtcggcttt caggtgggtg gcgccgctgc
    15661 ccagcagcct gccacgcgcc ccgccggtgt tgagaccgaa ccggcgccgc ccggcacgca
    15721 gcccgcgccc ctgatcgcgc ccgaaacgcc cgagcagccc tacgaagcgc cgcctacacc
    15781 cgacgtgtgc accagcgctc agcggctcca ctttgatgag ctgctgttcg acctgggttt
    15841 cgaagcacaa gccgatcgtc gcaaggttcg ctcacgcgtt tacgaccgct acttgaaacg
    15901 ggagtttcag aagaaaacca ctgccggcag cctgcaagag acagtggacg tgtacaccgg
    15961 cggctgggcg ttcggcgaac gcgtcctgcg attcgacgtc ggggcacgct gggggttatc
    16021 gcagaatcgc ttttccgagg tgagccccgc gcccgacatt accaaccgtc cgcacggcga
    16081 cgtgctcgaa tacgacctga agttcaagct gttcccgcgt ggcgtcgtct cggccacagc
    16141 cttcgcttcg cagttagaca gccgcgtgcc gcgtccgttc ctgcccagcc tcgaccgcac
    16201 ccaggaacgc tacggcacga gcgtcttcgt caacagccca accttcccga tgcgcttcag
    16261 cttcgagcat ttatgggaga acctaaccag ccgcacccgc gaactgaacg acgagcagca
    16321 atacggtgat gatcgcttcg aatacgaagg cacctggcag atcagcgagc ggcagtcgtt
    16381 acgcctcgaa taccggcact atgactacac cgaacgctat tccgggacca ggacgcagtt
    16441 caacacctcg ggcaattacc tcctggccaa ccatacgctg cgcttcgggc cggacgaccg
    16501 cagctcgctc gagaccctgg cccgcctgca ggacgaatcc ggcgacctgg agcaggacat
    16561 cgccgaggtt tccagccggc tgcgacttca gcacacggac tccttcgcga ccaactacgc
    16621 cgtgcagttc ttgcgcaacg cctttcacga gttgcagacc gagacttacc gcggcgaggc
    16681 cggcctgacg caccagctcg gcgaatgcct gacctccacg ctgcagttct acggcctcaa
    16741 gcaggacgcc aacaagaacg cggatttcac cgagtggaac actttggcca acctggcgtt
    16801 ctccaagccg aaccccttgg gccgcttctc cgcgaatctc agttacaatc acacggccac
    16861 tgacgccagc gacaataacc gacgcgggat cgcgatagcc gagtctgtca cgttccgcga
    16921 cccgttgccc gccttcctgg cgcagaccga cgtggacata accacgctgg tcgtaacgga
    16981 ttccacgcgg gcccgcgtat tcctgccggt acgggatttc atcgtcctga agctgggccg
    17041 ctacacggct ttgcagcgtg tcccgaccgg catgattcag gaccggcaga cggtactggt
    17101 cacctatacc tatcgcgtgt cttcaaacta cgacgtgcgc cgggaccgcg tcgactttcg
    17161 catgcagcag gaattcaagt gcggactgac gccctattac gcgggttcga tgcagaatga
    17221 agacgtaacg caggcccgat tcctgaggtt cgaaccgcgc aacgtgaacc gccaccgcct
    17281 cggagccacc taccgccggc cgcgctggtc ggtgggcggc gaatacgaat acaacgacga
    17341 cgccatcgag ccgtaccagg ccgtgcactt caacggcgac gtcgtgctgc tgcaaaagct
    17401 gcaacatcag ctcgacggca aggccacgct gtcgcggttt tggtttgacg attcgtacag
    17461 cttttcgcgg tggaacgact ccggcctgac gtgggccgag tccgtcggcg cgggccgctg
    17521 gaccagcacg ctgctggact tgggcgcggc ctaccgctat ctcgtcaggc gcgatctgga
    17581 ggccagtgcc agcgcgatgt accgttatca gcacgattcc attttcggcg acacccacgg
    17641 cgtcgacttg tcggccgcgg tcgaatggcg gatcggctac ttttcgctgc ggttcgaggc
    17701 ggaatacgac gtgctcgacc tgcccgattc gctggaccat ggggcgtcgt tctggatcaa
    17761 actgaagcgc gagatacccg tgattgcgag gacgccgcaa tgaggccggt gcctgcctac
    17821 accctattcg ccctattcgc cgcattcgcc gcatttgcgc tgtgtaccgg ctgtcagagc
    17881 ccgccgcggc cgatctttcc cgcaatctcg ccggacattg tctggcccct gccgccggat
    17941 cgcccacgca tcaagtacat cggcgaactt acgggcgaag cgagtctcgg tgcgaagccg
    18001 cgcggcatgc aggccctgcg tcaagtgctg accggcccgc cgcccacggt cgcgtttgtt
    18061 cgtccggcgg cggtagctgt cttgggcgag cgagtcttcg tcgccgatac gggcctggcg
    18121 ctggttcata tgctcgatct gggcgagcgt cgctaccgcg ccattcaagg caacccggcc
    18181 gatccgctcc gcattccaat cgacctggcc atcgtggatg acgccacgct cgtcgtcgcc
    18241 gatcgcagtc gcgccgcact ggacatattc gaccgcgatg ggaactggca ggcgacgcgc
    18301 cgctttcccg agatcaccgc ccctgccgcc attgcatacg acagtttcag ccggcggctg
    18361 tgggtggcgg attccgcgac ccattgctgc ctggccgtca ccgccttgga acgtgttacc
    18421 gccaggatcg gccaacgcgg cagtgaaccg cggcagttca acttccctac ggcgctcgcg
    18481 acgcatccgg ccgtcggcct ggtcgtcgcg gacgcgatga acttccgcgt ccaggttttc
    18541 aacgcctccg ccgaaccggt cgcagtcttc gggcagaaag gcgacgcggc gggggatttc
    18601 gcccggccgc gcgacgtggc gctagactcc gaaggccata tctacgtact cgacaatcag
    18661 tttgagaacg tgcagatttt tgatcgcggc ggccggctgc tgatggcatt cggctctgag
    18721 ggcaccggtc cgggcgagtt ctcgctgccg tcgggcatca ctatcgatgc gcaggaccgc
    18781 atttggattg cggatagcct taaccgccgg gtgcaggtgt tccagtatct tgcggagggt
    18841 gcctcatgat tgcgtcgtgg tccgcaaagc gaatgatagt gctggcggtt ctggcgattg
    18901 cggcgcgcgc cgacgagcgc atcgccaatt ccaagcatga cctgtcggcc atgggaccgg
    18961 gacccacgcg cgcggtaaat gagagtgaaa tctgcatctt ctgccatacg ccgcataacg
    19021 ccagccccgt cgcgccgctc tggaatcgct acaaccccac gacctactac cgcatctatc
    19081 gcagccgtac gctgcaggcg cgtgtcgacc agcccggccc ggccagcaaa ctgtgcctca
    19141 gttgccacga cggaaccgtc gcgctgggcc tggtcttgaa ccgccccttc accaatccga
    19201 tcccgatgaa ccacccgtac atcaccggac ccgccgacct gacgaccgac ttgtcggacg
    19261 accatccgat cggcttccgc tacgaccagc aactggccaa cgtggacccc caaatccgcg
    19321 agccgggact ggtcgacttc agaatcaaat tgggcgagcg cggcgagctg gaatgcaccg
    19381 cctgccacga tccgcacaac aacgagctcg gcaactttct gcgcatcacc caacgggaag
    19441 gcgcgctttg ccgcacctgc cacgatatgc gcggctggga gcactccgcg cacgctctct
    19501 cgccgcgtaa cgtcccgctg accgtgaccc agggcgagcg tctgccctac aactcgctgg
    19561 ccgaaaacgc ctgcctgtcc tgtcatgttt cgcatagcgc gccctggcgc gagcagttgc
    19621 tgcgcgatcg cccgtcgcgg ctgtgcctgg attgccataa cggcgtagct gccgtcgaca
    19681 tcgcgccggt gatcaaccag ctctcggggc accgcatgaa tccagtgtgg gagagccgcg
    19741 aacgcggcga actgggcttc cgcatttcgc cgttcactca atgcactgcg tgccacaatc
    19801 cacacgccgt ccgcctgaac atccccggcg aagcgctgag cgtggccttg aacacgccgt
    19861 tcgtgccgcc ggccatgcgc gacgtgccgg gcgtaaacct ggccggcgtg ccggtgccgg
    19921 tcgcaacctt ttactacgag gtttgcttcc gctgccatgc ggacttcccg gttctggtgc
    19981 gcgaccgcat cgtgcggcag atcgacacgt tgggaaacgt gcgccggcag ttcctgccaa
    20041 ccgttgcttc cgcccatccc gtcactttcc ccagccggcg ctctaccgat gtgcccagtc
    20101 tggcgccgga attccgcacg cggctgttca tcagttgcca ggactgccat aacaacccca
    20161 acgcctacga cgcgggtggc ggcctggcca gcggtccgca cacttcgcgc tacaacttcc
    20221 tcctggtggc gcaatacgaa acgcaggact tcactatgga atctccacag gcgtatgccc
    20281 tgtgctaccg gtgccacgac cgcacctcta tccttaacaa cgaaagcttc ccactgcatc
    20341 gcgtgcacat tgtgaatggc cgatcgccgt gcagcgcctg ccacaccgca cacggcgtgg
    20401 gcggcagcac gccccaacac gaccacctga tcaacttcga catctccatc gtcggcggcc
    20461 agcgcttcta caacgacacc ggccgattca gcggcacctg cacgctgacc tgccacggcg
    20521 tacagcacgt aaacttcacc tatgccccgt agacaaaggc gaccgacggc cccaacgccg
    20581 ctcgccgccg gcagagcaga gccggggcgg ggcgcccggg ctggtcgaga gggatcgagg
    20641 gagcaaggga tcgagggatc gagggggcaa gggtgcaaga gtgcaaagaa ttcgatcaaa
    20701 cggcgctttt gcggcgtttg gcttgcgcca ggggcaggtt gccgggttgc caaggtacgc
    20761 gcgctcgaat ttggcaaagt gccgataacg cgctgcgcga gagaggtgaa caggagacag
    20821 gtgagcagga gaaagtggtg ctgatgcagg ctcatgcggt ccctttctgc gcgcgggcgc
    20881 ggttctgcgc gctttcctag atatcatagg acgggaatcc cgggggggcc gccggggggc
    20941 tttcggctgc gggggtggat tgttggcggt tgggggtggt tgcgcggcac ggagagagtt
    21001 gttagttttt agtctttagt attggcggtg ggagcgcgtt cggggcgggg atgtcggggc
    21061 gggtatgtaa gaacactgag ggcatgattg attgaaccgc agaggacgcg ggggaccgcg
    21121 ggggaagacc ggtttggacg gggagggttg ttagatccgg cgggccggtg ccggcaggcg
    21181 ttagcggctg cggcaggccg tccgggagtg ctcccggcgg gcctgcgccc tgcgagtcgg
    21241 gcgggtcgga cccggcaatc gaaatgacgg catttgcgga tcgttgatcg gcgtgacaat
    21301 gccggccgcg ccttcgccgt cgaacgcaat tgtgccgcgc cggaaccggg tcagcccttg
    21361 acacggcgtc gcgtgcgaag cacgcggatc gcttcgcgat cccggccgcg aagcggccgg
    21421 cgccgtgtca agggtcagcc ggtgccgctg gtcgtacgaa taaacctcgt tgcgattggg
    21481 ccgctcgaaa tcctgcttga acagccggtt gccgaccgga tcatagccgt aggccacgtc
    21541 gtagaacgga tcgagcgctg cgccgacgtc accgtgctga atctgaagca gccggttatt
    21601 caggtcgtaa gcgaacattg acgcggcccc gttgctcagc gccgccgact cgcgccggtt
    21661 gccgtcatcg aacgtccact gcacgccgtt gcccaggccg tcgaaaacgc cttgcggccg
    21721 cgtgcgcgga ccgattagcc cttcttcact gccccggaat gaggccattt tcgccgtgca
    21781 gagtcaagcg cctctccaca ttcggggcag ctcccggaaa cgttgccagt cagatcgtag
    21841 ccgcactgcg tacatcgacc cgcgcggcga cgccgcaggc ggctcaaggc gaccaggggc
    21901 caaggggcgc ttccgatcat gaacacgaga gcgaaaagcc aaagcggcgc tgttgtagtg
    21961 aagcagaggt agtcatctgc ctcctcaact atgaccgtac tgggcatcct ccactgaaaa
    22021 tggaccagaa acaggggaga tcgggggccg gatatttttc ggctggaaaa gcgaaccgaa
    22081 tcgtaggtcc atcgccccgg gttcgcccgc tcatcggtca gaaacagtcc gccatggaac
    22141 gtaaagacgc ttagaagcgg agcgacctta tggtcctctc tcacatattc aacctcgagc
    22201 cggtcgcccg tcgagtaact acgtatccac aggagaacgc tgaccagaca caagactcca
    22261 ctaagtatgc tgagccagcg catagggcac atttctgttc aacctcgccc gccggacggc
    22321 tttaccgctg acgcaggttc tcagaagtca catagaacac aggtcctcga cgcttctcca
    22381 gtcagcgagc cctgtctggc caagaggaaa agtcaccgga actgtccgcg tcccgacgcc
    22441 gttttgtgcg atcttgtcca cgttcagtgt caccattccc atactgtcta ccaacagatg
    22501 actggtttca gagcctccgg agcccacttc ttcaccaggt cgtccagccg gcgcgcttca
    22561 tcatatgcct cgtctgcctc ttgcctttca gacggtgtca acggcccaac gaagttcctc
    22621 cagcgccacc acgcaagcca tgcatcgtgg taggcttgta gcaacagcgg tagccatggg
    22681 ttgggagccg caacaacagg cggcatgacg tcatgctgtt ctagaggcca atctccatat
    22741 ttccgccgtt gctcttcacc tttgttcctt cggtgctcat tctctacgct tcccgcatct
    22801 gtttctgact ccctctcact cttgggcttc ggcccgtaga tgccccgcgc cgcgtggcgc
    22861 atttcatgaa caagcgctga ttccggatcc cgcttgacgc cgtcgtcgta tgtctcctcc
    22921 ctattgggat catggctgat aacggctacc cggttgctaa ttttcatcgc cacgttgccg
    22981 tcgttaggac cgacaaccac cacgacgcca ccctcttcat cttggttcag cctttcgatg
    23041 tcttgagcgc cccggcccat gctgccgtca gggttgcgcc gtgtccgggc atctgctagc
    23101 tgccgctgcg ccttgtccat atcgtcaggc gtggaaccgc gacccggttg ccaccagatt
    23161 ttgagaccaa ggggatccgt ccagtagagg gggttcccga gaacgtaggc atacagactg
    23221 attccgttta cataccccag cggatcgcgc tgcatccagc gcccaagttc cggcgagaag
    23281 ctgcggaacg ggaagtggta catgccgacg ctcgcatcgt agcgctggcc ggtccaggtg
    23341 aacggattgc cgtaggccga ggttcttgcg cgcatccatg cgcccgcgcc accgctaccg
    23401 atggcctccc acgtctcgat gtacgtcttg ccgtacgggt cgtagtcgta gcgctccacc
    23461 agcttcgccg gaaccggttc cggagtcgca tccgggtcgc ccgcatccac cagccccacc
    23521 acgctgccca gggcatcgtg cacgtagtgc agcacttcct ccgtgcccgc ctccacgtcg
    23581 cccgcggccg tgaagtcaaa cagcgccagc ggctccggga agcgctcgcc ccagagaacc
    23641 tcgcgcgcca gcccccagtc gccgccgtcg cacgagatat actcagcaac cgcgttcaac
    23701 ccggagtaaa tgtgtcgcgt ctgaaccgtc accggcccgg catcacccgt acacggatcg
    23761 accgccacgc cgtactccgt gctcgcgatc cgccttccca gcgcatcgta ctcgatctcc
    23821 agcaacggat tgccgtcgat ttccgcgaca g
//
LOCUS       DAOSYS010000022        23828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1756_length_23828_cov_5.454297, whole genome shotgun sequence.
ACCESSION   DAOSYS010000022 DAOSYS010000000
VERSION     DAOSYS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1756_length_23828_cov_5.454297"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PLU99_02465"
     CDS             complement(<1..975)
                     /locus_tag="PLU99_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOO15948.1"
                     /translation="MSVSESLWEKLRHYRLTALLAPQSPEACVAAYELFEPLGVVLEI
                     ALRTSAALDGIAAVRRRHPNALLLAGTVLTASQAEQAIAAGAAGIVSPDYLPPVVQVC
                     VARDVMCVPGGLGDVGKQLAQKAEGYGCTLAELRKHHPYQWVHKLFPVTAGGALLVDV
                     AAAWQAVYPELTVMYTGGVTFERLAEIVRRDPRAIICGSALTRRIEDAQATAAEAQRW
                     LDVIHGRASGTPQHAPRASTSSGSAAQPPPAAGAVVTFGEIMLRLTPPAGRRFSQAGS
                     LDASFGGAEANVAVALAQWGVPSRFVTAVPDQALGHAAIEALRAHGVDT"
     gene            complement(1001..1786)
                     /locus_tag="PLU99_02470"
     CDS             complement(1001..1786)
                     /locus_tag="PLU99_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOO15949.1"
                     /translation="MSNHPVAFVTGASRGIGRGIALALAASGFDVVGNARTYDPKDRH
                     TGLAEVQDRARELGVEFEPVAGDIANLESHPRLLQAALQRFRRVDVLVNNAGIAPQKR
                     LDVLETTPESFDRLLAVNARGPFFLTQCFARHMLSQPRGPLTPAIIFVSSISAEVSSP
                     SRAEYCISKAALSQAATVFADRLAEAGINVYEVRPGIIATDMTAPVKAQYDARIAAGL
                     VPLRRWGQPEDVGRAVAALARGDLGYATGQVIELSGGMNIRHL"
     gene            complement(1823..3847)
                     /locus_tag="PLU99_02475"
     CDS             complement(1823..3847)
                     /locus_tag="PLU99_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO15950.1"
                     /translation="MTSLPAEVLAAGAELQLYSTNTLIVGSGAAGLNAALQLVAHGQR
                     DVILVTERWGAGASNEAGSDKQTYYKLALAHAALDSPRQMAEDLFAGGSMHGDIALCE
                     AQHSAQAFYHLVARGVPFPHDRYGAYVGYRTDHDQRGRATSAGPLTSHLMCECLGRAV
                     REAGIKVLDQHQVVALLTYEARSEKHVCGAVALDMNNLDSPHHGLVVFNAVNVILATG
                     GPGGMYRTTVYPHSQVGSTGMALAIGAAANNLTESQFGLASTKFRWNLSGTYQQVIPR
                     YVSTDANGGDAREFLNEAFPDLRSLAGAIFRKGYEWPFDCAKVRGHGSSLIDLLVYQE
                     TVRRGRRAFLDFTQNPTDPAGREPFSLDCLDEEARTYLQRSGATAATPIERLRQMNEP
                     AYQLYRSHDIDLVREPLEIAVCAQHNNGGLAGNIWWESNIRHLFPIGEVNGTHGVRRP
                     GGAALNAGQVGGLRAALFIARRYGVAPPAANDFVKRVEAQLADTLEFARSVTARSSGG
                     LKPEQVIAEIQERMSSCAAIVREVSAIRAAAEAAWRLHKRVQRESSGSLPLAFRARDM
                     CLTHAVYLEALREYLERGGQSRGSYLVLDARGATLDANLDEEWRFGLAHPADFVSQNI
                     LEIELAADQHADVAAEQHVQKRWVPIRPIPKPDGWFENVWNDYRHDRIVQ"
     gene            complement(3844..5247)
                     /locus_tag="PLU99_02480"
     CDS             complement(3844..5247)
                     /locus_tag="PLU99_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15951.1"
                     /translation="MAASQHERFRLVDLAGLAACATRIGVSIPYSDDVRVLLSPLQLA
                     GRRLPNRFAVQPMEGCDAEPDGAPGPLTLRRYQRYAAGGSGLIWYEATAVTQEGRANP
                     RQLWITAANVGAFRTLVNETRAAARDCWGTAHDPLLILQLTHSGRYARPDGRPRPVIA
                     QHNPILDPRLGIGPDYPLVTDAELNRLQDAFVSAAELAAAAGFDGVDIKACHGYLVSE
                     LLGAFTRPHSRYGGPFENRARFLLEVVQRIRAQVPAVFVTSRFNAHDGLPHPHGFCND
                     EEALTEACELARRLRALACPVLNVSLGNPYYNPHFGRPYDSPIAWAATPDEHPLAGVA
                     RLLQAAATVQRAVPDVPVIGAGYSWLRHLFPHVAAAVVSAGNAALVGLGRMALAYPDF
                     VKDLAQNGALDPKKTCTACSGCSQIMRDGGRAGCRVRDPEIYAKEYREGRARAVSQSK
                     TGPTQSSGPAPAEASRS"
     gene            complement(5261..6262)
                     /locus_tag="PLU99_02485"
     CDS             complement(5261..6262)
                     /locus_tag="PLU99_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO15952.1"
                     /translation="MTSVFRVGVDSYSLGPLRLNPLALLDWAKANGADGAHFTDIYLE
                     PGQTLNDELLTQVGQRAAELGLYVEWGGGQHIPFDTNTWQPKDLRPINEAAARQARLV
                     GARVVRSCSGGLMRWSDQAPPTETLLRETARALRAQKAMLTELGIVLAIELHFEFTTF
                     ELLRLFDMCDAEPGGCLGICLDTMNLLTMLEDPVAGTERILPWVVATHAKDGALRLTD
                     AGLESFTTEIGNGVVNFPRILSRLANLDRPIHLSVEDHGGSFALPMYDPTFLSRFPDL
                     TAMELARLVQLAHRTPPAAAPLERAEWPKQCSSRLSRDIANLKAIVERWSSSTGRAV"
     gene            6456..8159
                     /locus_tag="PLU99_02490"
     CDS             6456..8159
                     /locus_tag="PLU99_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HOO15953.1"
                     /translation="MRGVLCFLVTVLGMGGWQLAGAGSPQPDSGGEAEVRFRKLLETY
                     VARFQPLHIERERADWAANISGSDADFARKQAAEQALIELRSDRAVFAELKQLKESGQ
                     VTDPLLARQLDVMYRAFLPGQADPELQKRIVALESEVEQTFNTFRATVDGKTLSENDV
                     RQIIATTKDPAAAEAAWKAYMEVGRRVEPKLRELVKLRNEMARQLGFDNYQALRLKTQ
                     EIDRDELIKLFDELDALTAAPFAELKAKLDARRAAWFGIGVAELRPWNYGDLFFQEAP
                     PAEGSSLDDVFKNADLVEIATRYFDGLGMQVADILARSSLYEQPGKTPHAFATDIDRA
                     GDVRILLNLQPNAYWADTILHELGHAVYDVYIDSSLPFLLRTASHGITTEGVALMFGA
                     MSKNEQWLKEFLKLEAAEAARAGAAAREALQAEKLIFSRWAQVMVRFEQGMYSDPDQD
                     LGKLWWDLKRKYQLLNPPKDTGRPDYAAKAHIVTTPIYYHNYLMGELFAAQIRAQIAK
                     DVLGGADPVRASFINQPAVGAWLRERVFAPGNLYSWNELTRRATGAPLSATFFAQEYV
                     R"
     gene            complement(8223..8651)
                     /gene="ruvX"
                     /locus_tag="PLU99_02495"
     CDS             complement(8223..8651)
                     /gene="ruvX"
                     /locus_tag="PLU99_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOO15954.1"
                     /translation="MPRWMGVDYGSRRIGVAISDYGGSIASPAAVLPGSGSLSGDARQ
                     IVAWARKNEVSAIVVGLPLNMDGTVGPQADISTKLAAEIAQAAPEWPVELWDERLSSF
                     EADANLAAAGARPSRRRELRDALAAQVILQSFLDSKRAGK"
     gene            9148..12972
                     /gene="rpoB"
                     /locus_tag="PLU99_02500"
     CDS             9148..12972
                     /gene="rpoB"
                     /locus_tag="PLU99_02500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOO15955.1"
                     /translation="MLINSVRDFSRHGDAVPVPNLIEIQTESYDRFLQVKVDPSKRQP
                     IGLEALLREVFPIESYDGNMRLEYVDYQLDPPRYTPDEARELGLTYGRPFRVRVRLHR
                     KDVEEVKEEAIYLGEIPIMMGGGEFIVNGSERVIVSQLHRSPGVDFVKESAEGDRALH
                     ACRIIPERGSWIEINVTRKDVLAVRIDQSSKIPATTFLRAMDPRYGSDEAVVKTFYAS
                     KTVRTQALKPDMYAAAAIVDPETGEELVRAGAQVGEALNKIRESSIKQVDVIAKMVDP
                     IILNTLAEDSTHTHEEALMKIYARLRPGNPVQLEKARKLFQEKFYDDARYRLGKVGRF
                     RLNRKFEQGIPETEMVLRPEDFVSSLKYLLALRSGDGQVDDIDHLGNRRLRTIDELAS
                     DEMRKGFLKLRRTVQERMSLKDPESIGKVAELVNTKSISSAIDFFFGRSELSQVVDQT
                     NPLAQLTHERRLSALGPGGLNRKRAGFEVRDVHISHYGRICPIETPEGVNIGLIASLG
                     IYSDVDEYGFLITPYRKVERGKVNGEVVYLRADEEMKTVLGPPDVVDEGKDGELHPVV
                     PREEYLRRMRDGSDGARKPRKLKDGAVLARVHGELQQSTSDDIEYVDISPKQTVGVSA
                     ALIPFLEHDDANRALMGSNMQRQAVPLVRTEPPIIATGMERAVAENSGMVVRARTSGK
                     VTYVDARRIVINDTDEYDLRKFVGLNERTCLNQKPIVKPGQQVKAGEVIADGAATHQG
                     ELALGRNVLVAFMTFDGYNFEDAILISERLVKTDTFTSIHIESHDVEIRETKLGREEF
                     TRDIPNVSERALRNLDDKGIVRIGTRVGPGDILVGKVSPKSKSELSPEEKLLHAIFGR
                     AGEDVKNDSLELPAGQEGIVIGTRHFSRRTHLSEEQKRELERQMKAFQADCDARRIKL
                     FRQMVTAIEAATEQVLIDPNTRQKVGRSENPEVIREQIDTFSLKWVKPASKRDEAEAI
                     YNQYWPKIADIASECERRLGHMKRGDELPSGVLEMVKVYVATKRTLSIGDKMAGRHGN
                     KGVIAKILPEEDMPFLADGTPIDICLNPLGVPSRMNVGQILETHLGWAAAVLGFQAVT
                     PVFDGAKEAEIKACVDEANALVRKRRQEPDVVRNAGQTRELLVEMPRSGKITLYDGRT
                     GEPFDQPVTVGYMYLLKLHHLVDDKIHARSTGPYSLITQQPLGGKARTGGQRFGEMEV
                     WGLEAYGAAYVLQELLTVKSDDVEGRTKIYESMVKGTHTLDAGTPVSFDVLCNEIRGL
                     GLNIQLEKSRLG"
     gene            13018..13548
                     /locus_tag="PLU99_02505"
     CDS             13018..13548
                     /locus_tag="PLU99_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15956.1"
                     /translation="MSEVVYGTGTDVTPAELAEFYIQVQHDIAAQPQQIEKMLANSAT
                     FVTARLDGKLIGIARGVCDGLRGYLTECKLDPRYQGPAAVTKKDGRIEHDTNGIALEM
                     ARRVLGKLFTDGIRRVDVVAWGTEVDFLEELGFQKQGGLVGMTMRADQWPGSTAPAGS
                     SRAAGQAVSSNGARND"
     gene            13598..18013
                     /gene="rpoC"
                     /locus_tag="PLU99_02510"
     CDS             13598..18013
                     /gene="rpoC"
                     /locus_tag="PLU99_02510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOO15957.1"
                     /translation="MAETVYDRINDFTAVRISLASPNDIRAWSFGEVKKPETINYRTY
                     RAEKDGLFCERIFGPERDWECFCGKFKGTKHKGMICDRCGVKVTHSRVRRKRMGHINL
                     AAPVVHIWFFKAMPSRLGTLLDMKTSDLEKIIYFQDYVVVDPGASKLERKQLLTEEEY
                     RRAIDEYGDTGFRALMGAEAIRELLTQIDLSALSAELLDGLRKTGSKQKIKELSQRLK
                     LVEAIRQSNNQAEWMVLEVIPVIPPDLRPLVLLDSGNFASSDLNDLYRRIINRNNRLK
                     KLIDLNAPEVIIRNEKRMLQQAVDALFDNGRCRRPVLGSSNRPLKSLTDMIKGKQGRF
                     RENLLGKRVDYSARSVVVVGPELKLWQCGLPKKIALELYQPFIIRKLRERGLADTIKS
                     AKKMLERREQPIWDILEEVIYQHPVLLNRAPTLHRMGIQAFEPVLVEGNAIRIHPLVC
                     RGFNADFDGDQMAVHLPLSIEAQAESHVLMMSTNNIFSPANGAPIMSASQDIVMGIYY
                     LTCEHAGEPGERPVGQERAFASVSEALMALHLRQIGMHTPIRVRVPRTRVIEGRSKPA
                     VPAPKNGLVRTTVGRCIFNDILPPEMPMYNYPLGKAISRVIDDCHHQLGRAATIKLLD
                     KIKELGFRWSTLAGLSFALTDLRIPDAKPKLVEAAQKLVDRVEKNFQSGNITPMERHN
                     QLIDIWVHARERVTQEMMKTLSEDWRTEDGAEAKKDAPKAKPYLNPIHLMTDSGARGS
                     VDQIRQLAGMRGLMAKPSGEIIEAPIKANFREGLSVLEYFSSTHGARKGLADTALKTA
                     DSGYLTRKLADVAQNVIINELDCGTIESVSKSTIYRGEEVDVKLSELIIGRVARDTIR
                     NPVTDEIIVRENELITPEIARRLEAKPEEGGLGLDSIRVRSPLTCESPQGICARCYGA
                     DLSTGMLVEEGLAVGIIAAQSIGEPGTQLTMRTFHTGGVASRAVVENEITTAAGGTIK
                     FDLNAIEMTDPETKQSFFRVLKRNGEIAINDEKGRELERYKVPYGAAVLAGDGQTVPP
                     RTVIVRWDPHFTPILAEAAGFVRFQDIVEGETVRPEQEGRHDSKRIVVEHKGDKHPRI
                     MVEDANGTILDFHHLPAKARIEVQEGQEVAPGTLLARQPREIAGTQDITGGLPRVTEI
                     FEARKPKEPSVMAEISGTVELHTDKRRGKMTIVVRSESGMEKEHHVPQDKQLLVHAGD
                     FIEAGTQLTSGPLIPHDILRINGEEDLQRYLLKEIQAVYRQQNVGINDKHIEIILTQM
                     LRKVKIENEGDSALLPNEVVDKFRFRQENARLARSVKVVDPGDTDFRVGQVVLRDDFA
                     AKNEQVEMDGGTPAKGRKPKPATAKTLLLGITKASLQSESFISAASFQETTKVLTQAA
                     LAGAEDRLVGLKENVILGRLIPAGTGFKAYKDLKLRYMGQPIEPIPILQQLGEAPTPV
                     VPGLAPLDAAAASLGGDGSMAQQEATPAVAGGTNP"
     gene            18341..18841
                     /locus_tag="PLU99_02515"
     CDS             18341..18841
                     /locus_tag="PLU99_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15958.1"
                     /translation="MLATVSFVKAALSMCAGMGMLTPCQLGGLADSGPLAEEWPINAK
                     PGECYALVYVPPEYATETFAVVPFEYEGVEEVQGGQPASEFFLAQDRRPAHETFQIDF
                     PWKSPRAAQMSMSADYRTVSRQRLVPAHTTRTAEYTEVERTIKVSEGRLLWKQVSCDN
                     GDDLGV"
     gene            19362..20123
                     /locus_tag="PLU99_02520"
     CDS             19362..20123
                     /locus_tag="PLU99_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15959.1"
                     /translation="MFANAMKAMCALSVLGCLGMGAPPADTKCSPCKNKAKPSCKPAC
                     KAAVADADALPPNAKPGECYAKFYVPPEYKTVTERVMVRQAYEEMEVIPAQYEWVEER
                     VCVKDASEQLRVMPAEFAMRELTFEQRPAETVWEVNKNSTCVADSSQPARDVFCLVNR
                     AAVHQTLQTQCLAKPAHVEKECVPAEYQTIRRQKLVAPATTRRVCIPAEYADIQKTIK
                     VCDGKMVWKRVDCDLNQGVVSQAPPKAVGSLVAWE"
     gene            20251..20823
                     /locus_tag="PLU99_02525"
     CDS             20251..20823
                     /locus_tag="PLU99_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOO15960.1"
                     /translation="MPRALSGITLAAVLVCAGCAARNGQTEVRGHEPEVKSRQVGFKN
                     QPQRDGTDFESVQARGAAVHDSDGALVGYRLDSAPDPLHEATLAQVRECVLHETGVLI
                     DVRPAEFFARGHLRGALNLPAGPADQMDLHLEQIRASIDPGELIILYCSGPGCGSAHM
                     VSEYLGGHGFTNTLIYSPGWKSLAAEKDLH"
     gene            complement(20897..23092)
                     /locus_tag="PLU99_02530"
     CDS             complement(20897..23092)
                     /locus_tag="PLU99_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO15961.1"
                     /translation="MSSGTSDGAGLKHFRRSLTFLRPQRRLVIMGLLCAVGVSLSYTA
                     SISALIPVLKIMFAEHETVADWLNRTETERRLGIVIGSDLPDDPAGIMIDRLQHSATA
                     SKLRPGQRIAALDGQPAGSHELMSRLASATGDTARVALRNPDGTTYETELPLQPYRSW
                     WQKLHKLAALLPADRDPRGRLITLALVMAALVASSIFGGLCRFANEGLIQTAVQRAMH
                     AFRTRVGDHVLRLPMQWHALQAAGDTLARLATDISKVEVGQSTLFGKVIREPLKAVCV
                     LALTLFIEWRLLVAAMFGLPVGWLLLRTFGRLVKRAQRGASQSWGRLLDHLGERLAGI
                     RIVKACNMQQAECRRFEREGAILTRAQTHIELVDAATNPALEILATTAIAGFILYGGY
                     RVFQQQLEPSLFFAAVICLGGVFDPIRKMGNVTNRLQAADASARRLFELLDLPAEEQN
                     GTAHDGPPATRADLPPFRETIEFRNLCFAYAANARPVLQDINLTISKGECVALVGPNG
                     SGKTTLVSLLMRFFEPTSGQIFIDGRDIADFTMHSLRRQIGLVTQDAVIFTGTLRENL
                     SYGATNVSEDRLQAAARLAHVDEFARNLRIEDNGRITTGYDAHISARTLSGGQRQRIA
                     LARAVLPDPPILILDEATSQVDSESERRIQQALDDVTRGRTTFIIAHRFSTIARANRI
                     VVLNEGRIIGCGTHDDLLQSCPFYVSLCQTQFAHGFEPATVGASAAEPA"
     gene            23201..>23828
                     /locus_tag="PLU99_02535"
     CDS             23201..>23828
                     /locus_tag="PLU99_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOO15962.1"
                     /translation="METISLSGPDITEAEINAVVAVLRTPHLSLGPKVPEFEAALARR
                     LNVKFAVACNSGTSALHMAWRSIGLQPGDEVVTTPFSFIASANSIMFESGRPVFVDID
                     PRTWQIDAGLIEAAITPRTRAVLPVDVFGSTPDWDAISAIVRRHKLRVVEDSCEALGT
                     TRKGKPTGTFGEVGVFGFYPNKQVTTGEGGMLVTDNAEIAGLARSIRNQ"
BASE COUNT         4526 a         7378 c         7876 g         4048 t
ORIGIN      
        1 agtgtcgacg ccgtgggccc gcagggcttc aattgccgcg tggccgagtg cttgatccgg
       61 cacggcggtt acgaagcgtg acggaacgcc ccactgcgcc agtgccaccg cgacgttcgc
      121 ctccgctccg ccgaacgagg cgtccagact gccggcctgg ctaaagcggc gcccggccgg
      181 cggggtcagc cgcagcatga tctcgccaaa cgtgaccacc gcgcctgcgg caggtggcgg
      241 ttgagccgcg gacccggacg aagtgctcgc acgcggcgca tgttggggcg tgcccgacgc
      301 gcggccgtgg ataacgtcaa gccagcgctg cgcctcggcc gcagtggcct gggcgtcctc
      361 aatgcggcgc gtcagggccg agccgcagat gatggcgcgc gggtcgcgcc gcacgatttc
      421 agccagtcgc tcgaacgtca ccccgccggt gtacatcacc gtcagctcgg gatacaccgc
      481 ttgccaggcg gcggctacgt ctaccaagag cgcgccgcct gctgtgaccg gaaagagttt
      541 gtgcacccac tgatacggat gatgctttcg caactccgcc aacgtgcagc cgtagccttc
      601 cgccttttgc gcaagctgct tgccaacatc gcctaagccc cccggaacgc acatcacgtc
      661 gcgcgccacg cacacctgca ccacgggcgg cagataatcg ggcgaaacga ttcccgccgc
      721 cccggccgcg atggcctgtt cggcctggct ggccgtcagc acggtgccgg ccagcaggag
      781 cgcgtttggg tgacgccgac ggaccgcagc gatgccgtct aaagcggcgc tggttcgcag
      841 cgcgatctcc agcaccaccc ccagcggctc aaacagttca tacgcggcca cgcacgcttc
      901 ggggctttgc ggcgcgagca gcgccgtgag ccggtagtgc ctaagctttt cccaaagtga
      961 ctccgagacg ctcaccccgc ctctcctctc tctccgcggc ttacaagtgc cgaatgttca
     1021 tgccgccgct cagctcaatc acctggccgg tggcgtagcc gaggtcgccg cgggccagcg
     1081 ccgccacggc ccggccgacg tcttccggct ggccccaccg tcgcagcggg accagcccgg
     1141 cggcaatgcg cgcatcgtac tgcgctttca cgggggccgt catgtcggtg gcgattattc
     1201 ccggccggac ctcgtagacg ttgatgccgg cttcggccag gcgatcggcg aaaacggtgg
     1261 cggcctggct cagggctgct ttggaaatgc agtattccgc gcgcgagggc gaagatacct
     1321 cggcggaaat cgatgagacg aaaatgatcg ccggggtaag cgggccgcgc ggctggctga
     1381 gcatgtggcg ggcgaagcac tgcgtcagaa agaaggggcc gcgtgcgttt acggccagca
     1441 ggcggtcgaa gctctctggg gttgtttcga gtacgtcgag ccttttttgc ggcgcgatgc
     1501 cggcgttgtt gaccaacacg tcaacgcggc gaaagcgctg cagggcggcc tggagcagtc
     1561 tcgggtgcga ctcgaggttg gcgatgtctc ccgcaacggg ttcaaactcg accccgagtt
     1621 cgcgcgcgcg gtcctgcact tcagccagtc ccgtgtgtcg atcctttggg tcgtaggtcc
     1681 gggcgttgcc gaccacgtca aagccgctgg ccgccagcgc cagggcgatg ccgcgtccga
     1741 ttccgcgact ggcgccggtc acgaaagcca cgggatgatt gctcattttg cgctccaact
     1801 tactgcacga tgcggtctgt gcttactgca cgatgcgatc gtggcggtag tcattccata
     1861 cattctcaaa ccagccgtcc ggcttcggga tcggccggat tgggacccag cgcttctgga
     1921 catgctgctc agcggcgacg tccgcgtgct ggtcagcggc cagctcgatt tccaggatgt
     1981 tttgactcac gaaatccgcc ggatgcgcca ggccgaaacg ccattcctcg tccaagttcg
     2041 catcgagcgt cgcgccgcgg gcgtcaagga ccagatacga accgcgcgac tggccaccgc
     2101 gttcgagata ttcacgcaac gcttccaagt aaacggcgtg agtcaggcac atgtcccggg
     2161 cgcggaaggc aagcggcagt gagccggacg actcgcgttg tacgcgtttg tgcaggcgcc
     2221 atgccgcctc ggccgcggcc cggatcgcgc tgacttcccg cacgatcgcc gcgcagctcg
     2281 acatgcgctc ctggatttca gcgataacct gctccggttt cagtccgccg ctcgatcgcg
     2341 cagtcacgct acgggcgaat tccaaagtgt cagcgagttg cgcctcgacg cgcttcacga
     2401 agtcgttcgc agcgggcggc gctacgccgt accggcgggc gatgaagagc gcagcccgca
     2461 acccgccgac ttggcccgcg ttcagagccg cgccacccgg gcggcggacg ccgtgcgtgc
     2521 cgttcacttc gccgatgggg aacaggtggc ggatgttgga ttcccaccag atattgccgg
     2581 ccagtccgcc attgttgtgc tgggcgcaga ccgcgatttc gagcggctcc cggaccaggt
     2641 caatgtcgtg agaacggtac agttgatagg ccggctcgtt catctgccgc aggcgttcga
     2701 tgggtgtcgc cgcggtggcg cctgaccgtt gcagatacgt gcgcgcctct tcatccagac
     2761 aatccagcga gaacggctcg cggccggccg gatcggtcgg attctgcgtg aaatcgagga
     2821 atgcccgccg gccgcggcgt acagtttcct ggtaaacgag caggtcgatc agcgatgagc
     2881 cgtgcccgcg caccttagcg cagtcgaacg gccactcgta gcctttacgg aagattgcgc
     2941 cggccagcga gcgcaggtca gggaaggcct cgttgaggaa ctcgcgcgcg tcgccgccgt
     3001 tggcgtcggt cgagacgtag cgcggaatca cttgttgata ggtgccggac aagttccagc
     3061 ggaacttcgt cgatgccagg ccgaattgcg attcggtcaa gttgttcgcc gcggcgccga
     3121 tcgccaatgc cataccggtc gagccaacct ggctgtgcgg gtacaccgtg gtgcgataca
     3181 tgccgccggg accgccagtg gcgagaatca cgtttaccgc gttgaacacg accagcccgt
     3241 ggtgtggact gtccagattg ttcatatcca gggcgacggc tccgcacacg tgcttctcgg
     3301 agcgggcctc gtacgtcagc agcgccacaa cctggtgctg gtcgaggacc ttgatgcccg
     3361 cctcgcgcac tgcccgaccc aggcattcgc acatcaggtg cgaggtcaaa ggtccggcgg
     3421 atgtcgcccg gccgcgctgg tcgtggtccg tgcgatagcc gacatacgcc ccgtaccggt
     3481 catgcgggaa cggcacgccg cgggcgacga ggtggtagaa ggcctgggcg gaatgctggg
     3541 cctcgcagag tgcgatgtcg ccgtgcatgc tgccgccggc aaacaggtcc tcggccatct
     3601 gccgcggcga atcgagcgcc gcgtgggcca gcgcaagctt gtagtaggtc tgcttgtcgg
     3661 aaccggcctc attggacgcc ccggcgcccc agcgttctgt caccaggatt acatcgcgtt
     3721 gcccatgcgc gacgagttgc agcgccgcat tcagcccggc cgccccagag ccgacgatca
     3781 gcgtgttcgt gctgtatagc tgcaactccg ccccggccgc caagacttcc gccggcaagc
     3841 tggtcatgac cgcgaggcct ccgctggcgc tggcccgctc gactgtgtgg ggccggtttt
     3901 cgactggctg acggccctgg cgcggccctc gcggtactcc ttcgcgtaaa tttcaggatc
     3961 gcgcacgcga cagccggcgc gccccccgtc gcgcatgatc tgactgcagc ctgagcaagc
     4021 tgtgcaagtc tttttggggt caagggcgcc gttttgcgcc aaatccttca cgaaatcggg
     4081 atacgccagc gccatgcgcc cgaggccaac cagcgcggcg ttgccggctg aaacgaccgc
     4141 tgcggccacg tgcggaaaca gatgccgcag ccagctatat ccggcgccga tcactggaac
     4201 gtcgggcacc gcgcgttgaa cggtcgcagc ggcctgcagc aggcgcgcta cgccggccag
     4261 cggatgctcg tcgggagtcg ccgcccaagc gataggcgaa tcataaggcc ggccgaagtg
     4321 cgggttgtag tagggatttc ccaacgacac gttcaacacc ggacaagcca gagcgcgcag
     4381 acgccgcgcc aactcacacg cttcggtcag cgcctcttcg tcgttgcaga agccgtgcgg
     4441 atgtggcagg ccgtcgtgtg cgttgaaccg gctggtgacg aaaaccgcgg gtacttgcgc
     4501 cctgatgcgt tgcacgacct ccagcagaaa gcgggcccgg ttctcgaacg gtccgccgta
     4561 gcggctgtgt ggtcgcgtga acgcccccag cagttctgag accaggtaac cgtggcaggc
     4621 tttgatatcg acgccgtcga agcccgcggc ggccgccaat tcggccgcgc tgacaaatgc
     4681 gtcctgaagg cggttgagct cggcgtcggt gaccagcgga taatccggcc cgatccccag
     4741 ccgcggatcg aggatagggt tgtgctgggc gatcacgggg cgtggcctgc cgtcgggccg
     4801 ggcataacgc ccagaatgtg tcagttgcag gataagcagc ggatcgtgtg cagttcccca
     4861 gcaatcccgg gccgccgccc tcgtttcgtt caccagcgtt cgaaacgcgc cgacgttagc
     4921 cgcagtgatc cagagttggc gcggattcgc gcgcccttcc tgcgtcacgg ctgtagcttc
     4981 gtaccagatc agcccgctac cgcccgccgc gtaacgctgg tagcgccgca gagtcagcgg
     5041 ccccggcgcg ccgtcgggct ccgcgtcgca gccctccatt ggttgaacgg cgaaacggtt
     5101 gggcaaacgc cggccggcaa gctgcagcgg cgaaagaagg acgcgaacgt cgtcggagta
     5161 ggggatgctg acgccaatcc ttgtggcgca ggccgccagg cccgcaaggt cgacaagccg
     5221 gaatcgttcg tgctgcgatg ctgccacttc ctgccaccct ctacacggcg cgcccggtcg
     5281 aagaactcca acgctccacg atggccttaa ggtttgcgat gtcccgcgac aagcgtgatg
     5341 aacattgctt gggccactcg gcacgctcca gcggggcagc ggcagggggg gtgcgatggg
     5401 ctagttgcac caatcgcgcc aactccatcg cggtcaggtc ggggaagcgc gacaggaagg
     5461 ttgggtcata catgggcagg gcgaacgagc cgccgtgatc ctcgaccgat aggtggatcg
     5521 gccggtcgag gtttgccagc cgcgacaaaa ttcgcgggaa attaacaact ccgttgccaa
     5581 tctcggtggt gaacgactcc agccccgcgt cggtcaggcg cagtgcgccg tccttggcgt
     5641 gcgtcgcgac gacccacggc agaatccgct cggtccccgc caccgggtct tcgagcatcg
     5701 tgagcaggtt catcgtgtcc agacagatgc ccaggcatcc gccgggctcc gcgtcgcaca
     5761 tgtcgaacag ccgcaacagt tcgaatgtag tgaattcgaa gtgcaactcg attgccagca
     5821 cgatgcccag ttcggtcagc attgccttct gggcccgcaa agcccgcgcc gtctcgcgca
     5881 gcagcgtctc tgtcggcggc gcctggtcag accagcgcat cagcccgccc gagcacgagc
     5941 gcaccacgcg cgcccctacc aggcgcgcct gccgcgccgc tgcttcgttg atcggccgca
     6001 ggtctttggg ctgccacgtg tttgtgtcga acgggatgtg ctgtccgccg ccccattcga
     6061 cgtacaagcc gagctccgca gcccgctggc ccacctgggt cagcagctcg tcgtttagcg
     6121 tctgtccggg ctcgaggtag atgtcagtga aatgcgctcc gtcggcccca ttggccttcg
     6181 cccaatccag cagtgccaac gggttgagtc gcagggggcc caggctgtag ctatcgacgc
     6241 ctacgcggaa gacgctcgtc atggcgggct ctcctcctca cggcccgacc gcgccggcct
     6301 tcgcggcgcg tcggaatggg caggatacgc cgctgtgttg cttgctacaa cggtttgggt
     6361 cgaggaccaa ctgccaacgg cgctcggcgg gaacggacgc acaggccctg ctatagtgct
     6421 atactcgcga ttcgaagctt ctcggagacg gatgcatgcg cggtgttttg tgctttctcg
     6481 tgacggtgct aggtatgggc ggatggcagt tggccggcgc gggctcgccc cagccggata
     6541 gcggcggcga ggccgaggtt cggtttcgca agctgttgga aacctacgtg gcccggttcc
     6601 aaccgcttca tatcgagcgc gaaagggccg actgggcggc gaacatcagt ggcagcgatg
     6661 ccgacttcgc ccgcaagcag gcggccgagc aggcgttgat tgagctgcgc agcgaccgcg
     6721 cagttttcgc cgagctgaag cagctcaagg agagtgggca ggtcaccgac ccgctattgg
     6781 cgcggcagct tgacgttatg taccgggcgt tcctgccggg gcaggccgac cctgagttgc
     6841 agaaacgcat cgtcgcgctg gagagcgagg tcgagcagac cttcaacact ttccgcgcca
     6901 ccgtggacgg caagacgctc agcgaaaacg acgtgcgcca aatcatcgcc acgaccaagg
     6961 acccggccgc ggccgaggcg gcctggaagg cctacatgga agtcggccgg cgcgtcgagc
     7021 cgaaactgcg ggagctcgtc aaattgcgca acgaaatggc ccgccagctt ggcttcgaca
     7081 actatcaagc gctgcgcctc aaaacgcagg agatcgatcg tgatgagctg attaagctgt
     7141 tcgacgagct ggacgcgctg acggccgcgc ctttcgccga gctcaaggcc aagctcgatg
     7201 cacggcgcgc ggcgtggttc ggaattgggg ttgcggaact gcggccgtgg aactacggcg
     7261 atttgttctt ccaggaggcg ccgccggccg aggggtctag tctcgatgac gttttcaaga
     7321 acgctgactt ggttgaaatc gcgacgcgct atttcgacgg cctgggtatg caggtggctg
     7381 atattttggc gcggagcagc ctatacgagc agcccggcaa gacgccgcac gcatttgcga
     7441 cggacatcga ccgcgccggc gacgttcgca tcctgctgaa tctgcagcca aacgcctatt
     7501 gggccgacac gatcctgcac gaactcgggc acgctgtcta tgacgtgtac attgactcca
     7561 gcttgccctt tctgctgcgc accgcgtcgc acggcatcac gacggagggc gtcgccctga
     7621 tgtttggggc gatgtcgaag aatgagcagt ggctgaagga gtttctgaaa ctggaggcgg
     7681 cggaagccgc ccgcgcaggc gctgcggcgc gcgaggcgtt gcaggccgag aagctgattt
     7741 tcagccgctg ggcgcaggtg atggtgcgct tcgagcaggg catgtattcc gacccggatc
     7801 aggatctggg caagttatgg tgggacctca aacgcaaata ccagctcctt aatccgccca
     7861 aggacacggg ccgtcctgac tatgcagcga aggcgcacat cgttacgacg ccgatctatt
     7921 accacaacta cctgatgggc gagttgttcg cggcccagat cagagcacag atcgccaaag
     7981 acgtgctcgg cggggcggat ccggtgcggg ccagctttat taatcagccc gcggtcgggg
     8041 cctggttgcg cgaacgagtt ttcgcgccgg gcaatttgta ctcgtggaat gagctgacgc
     8101 gccgggcgac tggcgcaccg ctgtcagcca cgttctttgc ccaggaatac gtaaggtaaa
     8161 ccgccccgca aggctcgccg ggcgtggcgt gcagttgctg agctgactcc caaatttacc
     8221 ggctacttcc cggctcgctt tgaatctaga aaagattgca ggatgacctg ggccgccaaa
     8281 gcgtcgcgga gctcgcgccg gcggctgggg cgtgcgccgg ccgcggcgag gttcgcgtcg
     8341 gcctcgaagc tgctcagccg ctcgtcccac agctcaacgg gccattcggg tgcggcttgg
     8401 gcgatttcgg ccgcgagttt ggtcgaaatg tcggcctgcg ggccgacggt gccgtccatg
     8461 ttcaggggca gcccgaccac gatggccgaa acttcgttct tacgtgccca ggcgacgatt
     8521 tggcgggcgt cgccggacag gcttccggaa cccggcagga cggccgccgg ggaggcgatg
     8581 gagccgccgt agtcgcttat tgccactccg attcggcggg aaccgtaatc tacgcccatc
     8641 cagcggggca ttaggcgcgt tcctgtgcta ggggcaccgt gctaggggca cggccgtgaa
     8701 aacactgtcc gccggcggcg ccaagccgtg ccatgggcga gccggttctg ttggcggggg
     8761 catgcccgcg cttacagttt gggcatggca cccacgtggg gcacaaaccc acgttcggca
     8821 cccgtggcac ccgacggagg cgggagccat gtccggcagc cgacgtccca tatccgggct
     8881 aagcaatttt gttgccaggg cgatgaattt tgaaaaattg gcgcttgcaa aggtcaaaaa
     8941 cttcgctata gttacgaggc tacgcggtga tggctttggg actagcttca gcacggttcg
     9001 tcgcaactga cggccgaagg gttgcagtgc ctcaatggac caacaggtcc gatactcggc
     9061 ctacgtggga gctattatgc catctcccct caatcggggg ccgttgaact aagttgggca
     9121 agggcttggc cacgagggtg actcagcatg ctgattaatt ccgttcggga ttttagtcgc
     9181 cacggcgacg cggtgcccgt tccgaacctg atcgaaattc agacggaatc gtacgaccga
     9241 ttcctccagg tgaaggttga cccttccaaa cgccagccaa tcggcctgga ggccctgctg
     9301 cgcgaggtgt tcccgatcga gagctacgac gggaacatgc ggctggagta cgtcgattac
     9361 caactcgacc cgccgcggta cacgcccgac gaagcgcgcg agctgggact gacgtacggt
     9421 cgtccattcc gagttcgcgt gcgtctgcac cgcaaggacg tcgaggaggt caaggaagag
     9481 gcgatctacc tgggcgagat tccgatcatg atgggcggcg gggagttcat cgtgaacggc
     9541 tcggagcgcg tcatcgtctc gcagctccac cgctcccccg gcgtggactt cgtgaaggaa
     9601 tcggccgagg gtgaccgcgc cctgcacgcg tgtcgcatca tcccggagcg cggctcgtgg
     9661 atcgagatca acgtgacgcg caaggacgtg ctggcggtcc ggatcgacca gagcagcaag
     9721 atacccgcga cgaccttcct gcgggcgatg gacccgcggt acggctcgga cgaagcggta
     9781 gtaaagacct tttacgcctc gaagacggtc cgcacgcagg ccctcaagcc cgacatgtac
     9841 gccgccgccg cgatcgtcga ccccgaaacc ggcgaagagc tagtgcgggc cggtgcgcag
     9901 gtcggcgagg cgctcaacaa gattcgtgaa tcctcgatca aacaggtcga cgtgattgcc
     9961 aagatggtcg acccgatcat tctgaacacg ctggccgagg atagcacgca cacccatgaa
    10021 gaagccctga tgaagatcta cgcccgcctg cggccgggaa acccggtgca gcttgaaaag
    10081 gcgcgcaagc tcttccagga gaagttctac gacgacgccc gctatcgtct gggcaaggtg
    10141 gggcgcttcc gcctgaaccg caaattcgaa cagggcatcc ccgaaaccga aatggtgctg
    10201 cgcccggagg acttcgtcag ctcgctgaag tacctgctgg ccctgcgttc tggcgacggg
    10261 caggtggacg acatcgacca cctgggcaac cgccggctgc ggaccatcga tgagctggcc
    10321 tccgacgaga tgcgcaaggg cttcctcaag ctgcgccgca ccgttcagga gcgcatgagc
    10381 ctgaaggacc ccgagtcgat cggaaaagtc gccgaactgg tcaacacgaa gagtatttca
    10441 agcgcgatcg acttcttctt cggccgcagc gaactgtcgc aagtggtcga ccagaccaac
    10501 ccgctggcgc agcttacaca cgagcggcgc ctgtcggccc tggggccggg cggtctgaac
    10561 cgcaagcggg ccggcttcga agtccgcgac gtacacatca gccactacgg ccgcatctgc
    10621 ccgatcgaaa cgccggaagg cgtgaacatc ggcttgatcg catcgctggg catctacagc
    10681 gacgtggacg agtacggctt cctgattacg ccctaccgaa aggtggagcg cggcaaggta
    10741 aacggcgaag tcgtgtacct gcgggccgat gaggaaatga aaaccgttct cggtccgccc
    10801 gatgtcgtgg acgagggcaa ggacggcgag cttcacccgg tcgtgccgcg tgaggagtat
    10861 ctgcggcgaa tgcgggacgg gtcggacggg gcgcgtaagc ctcgcaagct gaaggacggg
    10921 gccgtgttgg cccgagttca cggcgagttg cagcaatcca cctcggacga catcgagtac
    10981 gttgacattt cgcccaagca gacggtcggc gtttcggcgg cgttgattcc gttcctcgag
    11041 cacgacgacg ccaaccgtgc gttgatgggc tcgaacatgc agcgtcaggc ggtgccgctg
    11101 gtgcggaccg agccgccgat catcgcgacc ggcatggaac gggcagtggc cgagaacagc
    11161 ggcatggtgg ttcgggcgcg cacgagcggg aaggtgacgt acgtcgatgc gcggcgcatc
    11221 gtgatcaatg acacggacga gtacgatctg cggaagttcg tgggcctcaa cgagcgcacc
    11281 tgcctgaacc agaagccgat cgtcaagccc ggccagcagg tcaaggcggg cgaggtgatc
    11341 gcggacggcg ccgccacgca ccagggcgag ttggcgctgg ggcggaacgt gctggtcgcg
    11401 ttcatgacct ttgacggcta caacttcgaa gacgccattc tgatctccga gcggctggtc
    11461 aagaccgaca ctttcacgag cattcatatc gagtcgcacg atgtggagat tcgtgagacg
    11521 aagctgggcc gcgaggagtt cacgcgcgac attcccaacg tttcggagcg cgccctgcgc
    11581 aacctggacg acaagggtat cgtgcgcatc ggcacgcgcg tcgggccggg cgacattctg
    11641 gtgggcaagg tttcgccgaa gagcaagagc gagctgtcgc ccgaagagaa gctgctgcat
    11701 gcgatcttcg gacgggccgg cgaggacgtg aagaacgact cgctcgaact gccggccggg
    11761 caggaaggca tcgtgatcgg cacgcggcac ttcagccggc gcacgcacct gtctgaagag
    11821 cagaagcggg aactcgaacg gcagatgaag gccttccagg ccgactgcga tgcgcggcgc
    11881 atcaaactgt tccggcagat ggtgacggcc atcgaggccg cgaccgagca ggtgttgatc
    11941 gacccgaata cgcgccagaa ggtggggcgc agcgagaacc cggaagtcat ccgcgaacag
    12001 attgacacct tctcgctcaa gtgggtcaag cccgccagca agcgcgatga ggccgaggcc
    12061 atttacaacc agtactggcc caagatcgcc gacatcgcca gcgaatgcga gcgtcgcctg
    12121 gggcacatga agcgtggcga cgaactgcct tcgggcgtgc tggaaatggt caaggtctac
    12181 gtggcgacca agcgcaccct gtcgatcggc gacaagatgg ccgggcggca cggtaacaag
    12241 ggcgtcattg ccaagatcct gcccgaagag gacatgccgt ttctggcgga tggcacgccg
    12301 atcgacatct gcctcaaccc gctgggcgtg ccgagccgta tgaacgtcgg gcagattctg
    12361 gagacgcact tgggctgggc ggcggcggtg ctgggcttcc aggccgtcac gccggtcttc
    12421 gacggcgcca aagaggccga gatcaaggcc tgcgtggatg aggccaatgc cctggttcgc
    12481 aagcgccggc aggaaccgga cgtggtccgc aatgccgggc agacgcggga gctgctggtc
    12541 gaaatgccgc gcagcggcaa gataacactg tacgacggtc gcacgggcga gccgttcgat
    12601 cagccggtga ccgtgggtta catgtatttg ctcaagctgc atcacctggt ggatgacaag
    12661 attcacgccc gctcgaccgg cccgtacagc ctgatcacgc agcagccgtt gggcggcaag
    12721 gcccgcaccg gcggccagcg cttcggcgag atggaagtct ggggcctgga ggcctacggc
    12781 gcggcttacg tgctgcagga gctgctgacg gtcaagagcg acgacgtcga aggacgcacc
    12841 aagatttacg agagcatggt caagggcacg cacacactcg acgccggtac gccggtctcg
    12901 ttcgacgtgc tctgcaatga gattcgtggg ctggggttga acatccagct cgaaaagagc
    12961 cggctgggat aagtgaatgc agaaaagcgc attcgtaatt cccgaggagg cgggttgatg
    13021 tccgaagtgg tctacgggac cggaaccgat gttacgccgg ccgagctggc cgaattctac
    13081 atccaagttc agcacgacat cgccgcccag ccgcaacaga ttgagaagat gctggccaac
    13141 agcgctactt ttgtcaccgc ccgcttggac gggaagctga tcggcatcgc ccgtggcgtt
    13201 tgcgacgggc tgcgcggcta cctgactgag tgcaagctgg acccgcgtta ccagggtccg
    13261 gcggcggtga ccaagaagga cggccgcatc gaacatgaca ccaacggcat tgctctggaa
    13321 atggcccgac gtgtgctggg caagctgttc accgacggca ttcggcgtgt ggacgtagtg
    13381 gcttggggca ccgaggtgga tttcctggag gaactgggtt tccagaagca aggcggcctc
    13441 gtgggcatga cgatgcgggc cgaccagtgg ccgggcagca ccgcaccagc cggatcgtcg
    13501 cgggcggccg gacaggcggt gtcgtcaaat ggagcacgaa acgactgatc ggcggttacc
    13561 cgccgagtga gttttcaata gagaggtagt acagccgatg gccgagactg tttacgaccg
    13621 gatcaacgac ttcacggcag tgcgcatctc gctggccagc ccgaacgaca ttcgggcctg
    13681 gagcttcggc gaggtgaaga agccggagac gatcaactac cggacgtatc gagcggaaaa
    13741 agacgggctg ttctgtgagc gcattttcgg tccggagcgc gactgggaat gcttctgcgg
    13801 caagttcaag ggcaccaagc acaaggggat gatctgcgat cgctgcggcg tgaaggtgac
    13861 gcacagccgc gtgcgccgca agcgcatggg gcacatcaac ctggccgcgc cggtcgtgca
    13921 catatggttc ttcaaagcca tgccgagccg cctgggcacg ctgctcgaca tgaagacctc
    13981 cgacctggaa aagatcatct atttccagga ttacgtggta gtcgatccgg gcgcctccaa
    14041 gcttgaacgc aagcagcttc tgactgagga agagtaccgc cgggcgatcg acgaatacgg
    14101 cgacaccggc ttccgcgcgc tcatgggcgc cgaggcaatc cgcgagctgc tcacgcaaat
    14161 cgatttgagc gcgctttcgg cggaactgct ggacgggctg cgcaagaccg gcagcaagca
    14221 gaagatcaag gagctgtcgc agcggctcaa gctggttgag gccattcgcc agagcaataa
    14281 ccaggcggaa tggatggtgc tggaagtcat tccggtcatc ccgcctgacc tgcgcccgct
    14341 ggttctgctg gatagcggca acttcgccag cagcgacctg aacgacctct accgtcgcat
    14401 catcaaccgc aacaaccgcc tcaagaagct gatcgatctc aacgctcccg aagtgattat
    14461 ccgcaacgag aagcggatgc tgcagcaggc ggtcgacgcc ctcttcgaca acggccgctg
    14521 ccgccgcccg gtgctgggca gcagcaaccg cccgctcaag tcgctgaccg acatgatcaa
    14581 gggcaagcag ggccgcttcc gcgagaacct gctcggcaag cgcgtcgatt actcggcccg
    14641 cagcgtggtc gtggtgggcc cggagctgaa gctgtggcag tgcggtctgc cgaagaaaat
    14701 cgcgctcgaa ctctaccagc ccttcatcat tcgcaagctg cgcgaacgcg gactggcgga
    14761 cacgatcaag agcgccaaga agatgctcga gcgccgcgag cagccgatct gggacatcct
    14821 cgaagaggtc atttaccagc atccggtcct gctcaaccgc gccccgacgc tgcaccgcat
    14881 gggtatccag gccttcgagc cggtgctggt tgaaggtaat gcgattcgca ttcaccccct
    14941 ggtctgccgc ggcttcaacg ccgacttcga cggcgaccag atggccgtgc acctgccgct
    15001 gtcgatcgag gcccaggccg agtcgcacgt gctgatgatg agcaccaata acatcttcag
    15061 cccggccaac ggcgccccga tcatgtccgc ctcgcaggac atcgtcatgg gtatctacta
    15121 cctgacctgc gagcacgccg gcgagcccgg cgagaggccc gttggccagg agcgcgcctt
    15181 cgcctcggtg tccgaggccc tcatggccct gcacctgcgg cagatcggca tgcacacccc
    15241 gatccgcgtc cgtgttccgc gaacgcgagt gatcgaaggc cggagcaagc ccgccgttcc
    15301 ggcgcccaag aacggcctgg tgcgcaccac ggtcggccgc tgcatcttca acgacatcct
    15361 gccgcccgag atgcccatgt acaactatcc actgggcaag gccatctcgc gcgttatcga
    15421 tgattgccat catcagttgg gccgcgccgc gacgatcaag ctgctggaca agatcaaaga
    15481 actgggcttc cgctggagca cgctggccgg cctatccttc gcgctgaccg acctgcgcat
    15541 tccggacgcc aaacccaagc tggtcgaggc cgcccagaag ctggtcgatc gcgtcgaaaa
    15601 gaacttccag agcggcaaca tcacgcccat ggagcgtcac aaccagctca tcgatatctg
    15661 ggtccacgcc cgcgaacggg tgacgcagga aatgatgaag accctgagcg aagactggcg
    15721 caccgaggac ggggcggagg ccaaaaagga tgcccccaag gccaagccct atctaaaccc
    15781 gattcacctc atgaccgatt ccggcgcccg tggctcggtc gaccagatcc gccagctcgc
    15841 cgggatgcgc ggcttgatgg ccaagccgtc cggcgaaatc atcgaggccc ccatcaaggc
    15901 caacttccgc gagggcctgt ccgtgctcga gtacttcagc tcgacccacg gcgcccgcaa
    15961 gggcctggcc gatacggccc tcaagacggc cgactcgggc tacttgacgc gcaagctggc
    16021 cgacgtggcc cagaacgtga tcatcaacga gctcgactgc ggcaccatcg aatccgtcag
    16081 caagagcacc atctatcgcg gcgaggaagt cgacgtgaag ctgtcggagc tgatcatcgg
    16141 ccgcgtcgcc cgcgacacca tccgcaaccc cgtcacggac gagattatcg tccgcgaaaa
    16201 tgagctgatc accccggaga tcgcccgccg cctggaagcc aagcccgaag aaggcggcct
    16261 cggcctggat agcatccgcg tccgcagccc gctcacttgc gaaagcccgc agggcatctg
    16321 cgcccgctgc tacggcgccg acctgtccac cggcatgctg gttgaagagg gcttggcggt
    16381 cggcatcatc gccgctcaat ccatcggcga gcccggcacg cagctcacga tgcgtacctt
    16441 ccacaccggc ggcgtcgcca gccgcgccgt ggtcgagaat gaaatcacca ccgctgccgg
    16501 cggcacgatc aagttcgacc tcaacgcgat cgagatgact gatccggaaa ccaagcagtc
    16561 gttcttccgc gtgctgaagc gcaacggcga aatcgctatc aacgatgaaa agggccgcga
    16621 gcttgaacgc tacaaggtgc cttacggcgc cgcggtgctc gccggcgacg gacagacggt
    16681 gcccccgcgc acggtcatcg tccgctggga tccgcacttc acgcccatcc tggccgaagc
    16741 ggccggcttc gtccgcttcc aggacatcgt cgaaggcgag acggtccgcc ccgagcagga
    16801 aggccgccac gacagcaaac gcatcgtcgt cgagcacaag ggcgacaagc acccgcgcat
    16861 catggtcgaa gacgccaacg gcacgattct cgacttccac cacctgcccg ccaaggcccg
    16921 tatcgaggtt caggaaggtc aggaggtggc gcccggcacc ctgcttgccc gccagccgcg
    16981 ggaaatcgcc ggtacccagg acatcaccgg cggtctgccg cgtgtgaccg aaatcttcga
    17041 ggcccgcaag ccgaaggagc cttcggtcat ggcggaaatc tcgggcacgg tcgagctgca
    17101 caccgacaag cgccgcggca agatgaccat cgtggtccgc tccgaatccg gcatggaaaa
    17161 ggagcaccac gtgccgcagg acaagcagct cctggtgcac gccggcgact tcatcgaggc
    17221 cggcacgcag cttacctccg gtccgctgat cccccacgac attctgcgga tcaacggcga
    17281 ggaagacctg cagcgctacc tgctgaaaga aatccaggcc gtctaccggc agcagaacgt
    17341 cggcatcaac gacaagcaca ttgaaatcat cctcacgcag atgctgcgta aggtgaagat
    17401 cgagaacgag ggcgattcgg ccctgctgcc gaacgaggtg gtcgacaagt tccgcttccg
    17461 ccaggaaaac gctcgcctgg cccgcagcgt caaggttgtc gaccccggcg ataccgactt
    17521 ccgggtcggc caggtcgtcc tgcgcgacga cttcgccgcc aagaacgagc aggtcgaaat
    17581 ggacggcggc acgcccgcca agggccgcaa gcccaagccc gccaccgcca agaccttgct
    17641 cctcggcatc accaaggcca gcctgcaaag cgagtccttc atctcggccg cctccttcca
    17701 ggagaccact aaggtgctca cgcaggccgc gctggccggc gccgaggacc gcctggtcgg
    17761 gctgaaggaa aacgtcatcc tcggccgcct gatccccgcc ggcacgggct tcaaggccta
    17821 caaggacctg aagctccgct acatgggtca gccgatcgag ccgattccga ttctccagca
    17881 gctcggcgaa gcgcccacgc cggtagtccc cggcctcgct ccgctggatg cggccgcagc
    17941 ttcgctgggc ggagatggaa gcatggccca acaagaagct acgccggccg tggcgggtgg
    18001 tacgaatccg tagcggatcg cgtgtgcgcg accggtgtac ggccggtctt tgaagtgcga
    18061 aaaagtaagc gagcagcgag gttaccgcct cgctgctcgc tgtcgtttcg aaaccacggc
    18121 gcagcccgcg attcccagct cggctcctaa attccgagtc atcatcgcta cccgcctttc
    18181 tcgctgtcat tcttacacct cgttcagtgc gaatttagcc gcacgtcatg acgcacgtgt
    18241 aagttacgag tgtgatctgc gagcagcgcg agctgatttg tctgccaacg caacacgagt
    18301 gcttatagac cggccaatgg caggcgtaag gaagtaagtc atgttagcca ctgtcagttt
    18361 cgtcaaagca gcgctgtcta tgtgtgccgg tatgggaatg ctgacgccgt gccagcttgg
    18421 cggcctcgcg gattctggcc cgctcgccga ggagtggccg atcaacgcca agccgggcga
    18481 gtgctacgcg ctggtttacg tgccgcctga gtacgcgact gaaacgttcg cggtggtgcc
    18541 cttcgagtat gagggggtcg aagaggttca gggtggtcag cccgcgtcgg aattctttct
    18601 ggcgcaggac cgccggccgg ctcatgagac gtttcaaatc gatttccctt ggaagtcgcc
    18661 gcgtgctgcg cagatgagca tgtcggcgga ttaccgcaca gttagcaggc agagactcgt
    18721 gccggctcac acgacgcgca cggctgagta cacggaggtt gagaggacca tcaaggtcag
    18781 cgaggggagg ctgttgtgga aacaggtgag ctgcgataac ggtgatgatc tgggcgtctg
    18841 agaggtccaa tagcgagcgg ggcgacctgc cgctggcggc aggcgctcac cgatagccgt
    18901 aattccggcc gcaaaccaag tcgtaattgg gggcgcgcgg ctcaacaact atcaatgcac
    18961 tgcgtccagg cgggtggaga caatgcgggg gtgagtcctg gtgtgccgat aatctcgctg
    19021 ttctgctcac gcagtgaaca tgaagtgaac gtgcgcatct ggcaaactgt gttcgtgcac
    19081 gctggccccc ttgcacaagt acaggacaca agagagtcac taggtacaga gaattcgaac
    19141 gatatgactt acgaggcggc gtgtgttggg ccctattaga cttgtgtgta ctgacctctt
    19201 accttatttc cgacgccaac ttctgacttt cgattcattc tgaatttatt ctcatgttac
    19261 agccgcggtg tacattaatt tgtggatcgg caacatagcc gttaagcacc gagtgcgagc
    19321 cggccaggcg gctgtgacga cgccacgagg aaggaaaggt catgtttgcg aatgctatga
    19381 aagccatgtg tgccctgtct gtgttggggt gtttgggaat gggtgcgccg cccgctgaca
    19441 ccaagtgttc accgtgcaag aacaaagcaa aaccatcgtg taagccggcc tgtaaagctg
    19501 cggttgcaga cgccgacgca ttgccgccca acgccaagcc gggcgagtgc tacgcgaagt
    19561 tttacgtgcc gcccgagtac aagacggtga cggagcgcgt gatggtgcgc caggcgtacg
    19621 aggaaatgga ggttattccg gcgcagtatg agtgggtcga ggagcgcgtg tgtgtcaaag
    19681 acgcttcgga gcagttgcgc gtgatgccgg cggaattcgc gatgcgcgag ctgaccttcg
    19741 agcagcggcc ggcggagacg gtgtgggagg ttaacaagaa ctcgacctgc gtggcggaca
    19801 gcagtcagcc ggcgcgagac gtgttttgcc tggtgaatcg cgcggccgtt caccagacgc
    19861 tgcagacgca gtgcctagcg aagccggcgc acgtggaaaa ggagtgtgtg ccggctgagt
    19921 accagaccat tcgcagacag aaactcgtcg cgccggctac gacgcgaagg gtatgtattc
    19981 cggcggagta cgcggacatc cagaagacga tcaaagtgtg cgatggaaaa atggtgtgga
    20041 aacgggtgga ttgcgacctt aaccagggcg tggtgagcca agcgccgccg aaggcggtcg
    20101 gttcgctggt cgcctgggag taagctagac gtcctccgag ccaggtcctg gcgcccgcca
    20161 ggacttggcg acggagactg gtccgaatga ccggggaact ggcaatgggc gccaagttgg
    20221 gagggattca gcgtgagaca gaggcttgat atgccgcgtg cactcagtgg cattacactt
    20281 gccgcagtgc tggtgtgtgc aggatgcgct gcacgcaatg gtcagacgga agtcagaggt
    20341 cacgagccgg aagtcaaaag tcgccaagta gggttcaaga atcagccgca gcgcgacggt
    20401 acagacttcg aaagtgttca ggcacgcggg gctgccgttc acgactccga cggcgcgctc
    20461 gtgggctacc gcctcgattc ggcgccggac ccgctccatg aggccacgct cgcgcaggtc
    20521 cgcgagtgcg ttctgcacga gaccggcgtc ctgatcgacg tacggccggc cgaattcttc
    20581 gcgcgcggcc acttacgcgg ggcattgaac ctgccggccg gaccggcgga ccagatggac
    20641 ctccacttgg agcagatccg cgccagcatc gatccgggag agctgatcat cctctactgc
    20701 tccggcccgg gctgcggctc ggcccatatg gtgagcgaat acctgggtgg ccacggcttc
    20761 acgaatacgc tgatatacag tcccggctgg aagagcctgg cggcagaaaa ggacttgcac
    20821 tagcgccact tgcaggagag caacgccggg gcgtgctgcc cggaatgcgc gccggcgaaa
    20881 gtcgccgtgt tcgctgctat gcgggttccg ccgctgacgc gccgaccgtt gccggttcga
    20941 agccgtgtgc gaactgcgtt tggcacagcg acacgtagaa cgggcaggat tgcaaaaggt
    21001 cgtcgtgcgt gccacagccg atgatgcggc cttcgttcag gaccacgatg cggttggcgc
    21061 gggcgatggt actgaagcgg tgggcgatga tgaaagtcgt gcggccgcgt gttacgtcgt
    21121 cgagggcctg ctgaatgcgg cgttcggatt cgctatcgac ctggctggtc gcttcgtcca
    21181 gaatcaggat gggcggatcg ggcagcacgg cccgggctaa cgcgattcgt tggcgctggc
    21241 cgccggagag cgtccgggct gagatgtggg cgtcgtaacc ggtggtgatt cggccgttgt
    21301 cttcgatgcg cagattgcgg gcgaactcgt cgacgtgcgc caggcgcgcc gcggcctgca
    21361 ggcggtcttc gctgacgttc gttgctccgt acgaaagatt ttcgcgcaac gtaccggtga
    21421 agatgacggc gtcctgagtc accaggccga tctgccggcg cagcgaatgc atggtgaagt
    21481 cggcgatgtc acggccgtcg atgaagattt gcccgctggt cggttcaaag aaacgcatca
    21541 gcaaggagac caacgtggtc ttgccggagc cgttcgggcc gaccagcgcg acgcactcgc
    21601 ctttgctgat agtcaagttg atgtcttgta gcaccgggcg agcgttggcc gcgtaggcga
    21661 aacagagatt gcggaactcg atcgtttcgc ggaagggggg caagtcggcc cgtgtcgcgg
    21721 gcgggccgtc gtgggccgtg ccgttctgct cctcggccgg caggtcgagc aactcgaaca
    21781 gacggcgggc tgaggcgtcg gccgcttgga ggcgattggt tacgttcccc attttgcgga
    21841 tggggtcgaa gactccgccg aggcagatca cggccgcgaa gaagagcgac ggttcgagct
    21901 gctgctggaa gacgcggtag ccgccgtaga ggatgaagcc ggcgatggcg gtggttgcca
    21961 ggatttcgag ggccgggttg gtggcggcgt cgaccaactc gatgtgcgtc tgggcgcggg
    22021 tcaggatggc gccttcgcgt tcgaagcggc gacactcggc ctgctgcata ttgcaggctt
    22081 tgacgatgcg aatgcccgcc aggcgctcgc ccagatggtc cagcagccgg ccccaggact
    22141 gcgaggcgcc gcgctgggcg cgtttgacga ggcgcccaaa tgttcgcagt agcagccagc
    22201 cgaccggcag gccgaacatg gccgctacca gcaggcgcca ctcgatgaac agcgtcaggg
    22261 cgagcacgca gaccgctttc agcggctcac ggatgacctt gccgaagagc gtgctttggc
    22321 cgacttcgac tttgctgatg tcggtggcaa ggcgtgccag cgtgtccccc gcagcctgaa
    22381 gagcgtgcca ttgcatgggc aggcgcagca cgtgatctcc gacgcgcgta cggaaggcgt
    22441 gcatggcgcg ctgcaccgca gtctggatca gcccctcgtt tgcaaagcgg caaagcccgc
    22501 cgaagatgga actggccacc agggcggcca ttaccagcgc cagcgtgatc aagcgcccgc
    22561 gcggatcgcg atcggccggc agcagggctg cgagcttgtg cagcttttgc caccacgagc
    22621 ggtaaggctg caaaggcagt tcggtttcgt atgttgtgcc gtcaggattt cgtaaggcaa
    22681 cgcgtgcggt gtcgccggtt gctgaggcga ggcgcgacat cagttcgtgc gagccggccg
    22741 gctggccatc gagcgcggca attcgctggc cgggccgcag cttgctcgcg gttgcgctat
    22801 gctggaggcg gtcgatcatt attccggcgg gatcgtccgg caggtctgag ccgatcacga
    22861 ttcccaggcg gcgctcggtt tcagtgcggt tgagccagtc ggcgaccgtc tcgtgctcgg
    22921 cgaacattat tttcagcacg ggaatcaggg ccgatatgct ggcggtgtac gaaagactga
    22981 caccgaccgc gcacagcagt cccataatga caaggcgccg ctgcggccgc aggaaagtca
    23041 ggctgcggcg aaaatgcttt aatccggcgc cgtcggatgt gcctgatgac ataggtcgat
    23101 atgataacag cttgccctcg caacgggcag gtgcgaatga agaaggagcc gaggggggcg
    23161 tcggggcaat aatttcgcgg cagagccgcg ggagagcagc gtggaaacga ttagtctttc
    23221 cgggccggat atcacagagg ccgaaatcaa cgcggtggtc gcagttttgc ggacgccgca
    23281 cctgagcctg gggcccaagg tcccggagtt cgaggcggcg ttggctcgcc gcctgaacgt
    23341 gaagttcgcg gtggcctgca acagcggcac gtcggcgctg cacatggcgt ggcgcagcat
    23401 cggcctgcag ccgggggacg aggtggtgac caccccgttt tcattcattg cgtcggcgaa
    23461 cagcatcatg tttgagagcg ggcggccggt gttcgtggat atcgatccgc ggacctggca
    23521 gatcgacgcc ggcctgatcg aggcggccat tactccgcgg acgcgagccg ttctcccggt
    23581 ggacgtattc ggttcgacgc cggactggga cgcgatcagc gcgatcgtgc ggcggcacaa
    23641 gctgcgcgtg gtggaagact cttgcgaggc cttgggcact acccgcaagg gcaagccgac
    23701 gggcaccttc ggcgaggttg gcgtgttcgg gttctatccg aacaagcagg tcacgaccgg
    23761 cgaaggcggc atgctggtca cagacaacgc tgagatcgcc ggactggcgc gttcgatccg
    23821 caaccagg
//
LOCUS       DAOSYS010000023        23721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1769_length_23721_cov_5.298572, whole genome shotgun sequence.
ACCESSION   DAOSYS010000023 DAOSYS010000000
VERSION     DAOSYS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23721
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_23721_cov_5.298572"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="fabG"
                     /locus_tag="PLU99_02540"
     CDS             <1..603
                     /gene="fabG"
                     /locus_tag="PLU99_02540"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOO15963.1"
                     /translation="SEHPDLADLIAPTALDVTDRAAVERVIEETLERHNRLDILVNNA
                     GITADGLVMGMKDEQFDSVLDTNLRAAFWAIRAVCRPMIRARRGRIINIASVSGIMGN
                     EGQANYAAAKAGLIGLTKSVAKEIAKRGVTCNAVAPGFIETDMTNVLPEELKARVKQV
                     VPMQRFGRPEEVAGMVAFLAGPAASYITGQVFVVDGGLHT"
     gene            765..2300
                     /gene="xylB"
                     /locus_tag="PLU99_02545"
     CDS             765..2300
                     /gene="xylB"
                     /locus_tag="PLU99_02545"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOO15964.1"
                     /translation="MPDSPLLLGIDVGTTGAKAILITAAGAVLAEATSEYPMATRRPL
                     WAEQNPHDWWSATVASVREVLHQAAAPAEAVAGVGLTGQMHGLVLLDEHGEVLRPCIM
                     WNDQRTGPQCAAITGQVGEQRLLQLTGNPVLPGFTAPKIVWVRENEPEVYGRIKHVLL
                     PKDYIRYRLTGEFFSDVSDASGTAMFDVGGRCWSEEMLAALDVPQEWLPEVTESLVAS
                     AAVSAAAAAETGLKAGTPVVGGAGDQAAQAVGTGVVSEGIVSVTIGTSGVVFAARNAY
                     RPEPRGRLHAFCHAAPGLWHLMGVMLAAGGSFRWYRDTLGEVERSQAAAGGGDAYDIL
                     TAEAAQAPAGCEGLLFLPYLCGERTPHPDPHARGAFVGLTLRHTKAHLTRAVMEGVTF
                     GLRDSLELMRGLGLEIRQVRASGGGARSPVWRQMLADAFGTEIVTVNMTQGAAFGAAL
                     LAGVGTGVYADVPSACQAVVKVSERCAPGPDEAVYARYYRRYQALYPALAQEFAALAE
                     AER"
     gene            complement(2351..3778)
                     /gene="gltA"
                     /locus_tag="PLU99_02550"
     CDS             complement(2351..3778)
                     /gene="gltA"
                     /locus_tag="PLU99_02550"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOO15965.1"
                     /translation="MSMTAKERMAISRQHMPEREPMERNRDFREVNLGFSVALAQLEA
                     NRCLQCKDRKCVSGCPVGIDIPGFLEKVSAGDLRGAAELLLSANALPGITGRVCPQEE
                     QCEQLCIRGKKGEPVAVGHLERFVADWARENLDMKQLIGPPTGFRVAVVGSGPAGLTC
                     AGELARKGHNVTVFEALHQPGGVLTYGIPEFRLPNKIIDAEVDRLRQMGVKIICNVVI
                     GRTFTVDELLNEEGFDAMFIANGAGLPIFMNIPGENLKGVYSANEYLTRSNLMRAYDF
                     PDFDTPIIRGERVVVIGGGNVAMDAVRTAKRLGAKEATLVYRRSRNEMPARVEEVKHA
                     EEEGIIFKFLTNPVRYLGTEDGWIRGVECQQMELGEPDESGRRRPVPVKGSEFVIECD
                     VAIPAIGTRANPLLTSTTPDLKTNKWGYIEVDENGATSKPGVYAGGDITRGSATVILA
                     MGDGKKAARSIDQYLAGKTPRTAKR"
     gene            complement(3918..4787)
                     /locus_tag="PLU99_02555"
     CDS             complement(3918..4787)
                     /locus_tag="PLU99_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOO15966.1"
                     /translation="MFKILDARQLASDVKWFRIEAPLVAKHRQPGQFVILRLHETGER
                     IPLTMANADRNTGTIELIVKAIGKTTRELCRKQAGDTIADVMGPLGRPTEIMRVDHAV
                     LVGGGVGTAVIYPLVTALREAGCFVSSITGGRTKELVVLEDELRRVSNVVYATTDDGS
                     YGFKGTVADKLRELIADKRRPIGVVYAAGPVPMMRAVCELTRSAGIKTIVSLNPIMID
                     GTGMCGGCRVTVGDKTKYACVDGPEFDGHLVNFAELSDRLTTYRKQEQISLERAEHAG
                     HQCRLNEPAKVSR"
     gene            complement(5006..6025)
                     /locus_tag="PLU99_02560"
     CDS             complement(5006..6025)
                     /locus_tag="PLU99_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO15967.1"
                     /translation="MRSARFRPILSVVVPVYNEAANVAAFLDRTLRVLQALNRDFELL
                     FVDDGSCDETASRITAAAVDCPAVRLVRLSRNFGHQAALVAGLAQARGAAVITMDGDL
                     QHPPELIPQLVAAWDAGADVVQAVRRAPVDDSPLKRAGSRAFYRLLSAVSRLAVTPGA
                     SDFRLMSRPAVDAFLACPERCRFNRGLVHWIGFTRDEILYEAATRHAGRSKYSWSAMI
                     RLAGDAIFSFSSVPLRLAGLAGACVSVVATFYLLFVLGAYFFTDRTVAGWASTLAAVL
                     VLGGVNLTVMWILGEYVGRLYEEVKQRPIYIVRPDGRADAQSGAEREGEPEEQPADRQ
                     ERVGV"
     gene            complement(6956..>8638)
                     /locus_tag="PLU99_02565"
     CDS             complement(6956..>8638)
                     /locus_tag="PLU99_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO15968.1"
                     /translation="GDAGQPATSSAPAEQPQEAEEREAVSKKDKKKKKIKPVEIELEG
                     FEARAVPLPVPAGNFSHLAVNDEGHLLYVRHPLRGSDDKPAIQIFDVKDDEKKEKTVL
                     KDVRGFDLSADGKKILVRKDGKLFIVKARPDQKTDKPMPTSGMVVSIDPRQEWREVFS
                     EAWRIMRDFFYVPNMHGVDWPGIRSRYGKMLEDCVCREDVSFIIKEMISELNVGHAYY
                     NATDPDQGPRVSVGLLGVDFELQDGAYRISKIYAGGPWDLDARGPLSQPGINVREGNY
                     LLAVNGLPLDTRADPWAAFQGLAGRTVVLTVSDQPALNDEARDVVVELLVSDRELRYR
                     AWIEAKRAYVDERSGGKVGYIYVPNTGRDGQADLVRQFHGQRFKQALIIDDRWNGGGQ
                     IPDRFIELLNRPLLSMWARRHGRPGRTPPQSHQGPKCMLINGLAGSGGDAFPAYFRQT
                     GLGKLIGTRTWGGLVGISGNPDFIDGRHMSVPTFGAYNLDSTWAIEGHGVDPDIEVID
                     DPSRMVDGGDPQLDRAIELMLEEIALHPFIEPPPPPPPDRSGMGLENERGKN"
     assembly_gap    8639..8738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8739..10330)
                     /locus_tag="PLU99_02570"
     CDS             complement(<8739..10330)
                     /locus_tag="PLU99_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HOO15969.1"
                     /translation="MRGCSLRGMSLVVALVWTAGLGMYAARGDVRPHVGMLVNPDVSA
                     THIVFSYANDLWVVPRDGGVAHPLSSPAGVESFPRFSSDGQTIAFMANYDGNTDLYTL
                     PVTGGVPFRVTHHPATENLCDFSPDGRLIFFSNAEAPFARMTQLFTVAPSGGLPEKLP
                     VPYGANAAISPDGEWLAYTLHTTDNRTWKRYCGGMATDIWLFNLKDHRAECITCWAGT
                     DSQPMWHGNMLYYMSDAGPEHRLNIWSYDRVSKERRQITNFAEFDIKWPAIGPGPNGE
                     GEIVFQNGTDLYLLDLASGQSRAVDVTIPGDRPKIRPQLIDVSSDIRSVTISPSGKRA
                     VMEARGDIWTAPARDGSPRNLTRSVGVHERYPSWSPDGRWIAYWSDATDENELYLVQS
                     DGKSEPRKLTNHGPGFRYGILWSPDSKYVVYGDNAGNLYLRAIEEDESKLVDTDPDAR
                     VPEASWSPDSRWIAYTKRTDSDLSTIWLYNAESGEKQQLTSALFNSSNPTFDREGDYL
                     YFITNRNVDTPIYTELGNTWVYV"
     gene            complement(10525..11493)
                     /locus_tag="PLU99_02575"
     CDS             complement(10525..11493)
                     /locus_tag="PLU99_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOO15970.1"
                     /translation="MKLGVMGAGLASLGWEKALDYCKTLGLEAIELPVGGYPGTPFFD
                     PEQLLREPAAQQKIKDDCARRGLAIIGLAVHGNPIHPDAAIAQQHLKAHETAVRLAPK
                     LGTDVVITFSGCPGGAPGDKTPNWVTAAWPPDFTKIQEYQWTEVLVPFWKKKAREAAD
                     EGVKIAFEAHPGFCVYNPDTLLRLRDACGPSLGANLDPSHFFWQGIDPIEAARVFGEA
                     GCLFHVHGKDTGVDPHNTRINGTLDAKSYGDLKARSWVFRTCGYGHGDEFWKPFISML
                     KRVGYDGVVSIEHEDAYMSVQEGFEKAVAYLRGVLIKEQPGAAWWF"
     gene            complement(11601..11885)
                     /locus_tag="PLU99_02580"
     CDS             complement(11601..11885)
                     /locus_tag="PLU99_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOO15971.1"
                     /translation="MVESTKPTIEQDQKTRRLPPFRVILHNDDVNTVDHVVRTIVKLT
                     PLNTEEAVARTLEAHETGSSMLLVTHKERAELYVEQFASCKLNVTCEPDS"
     gene            complement(12021..12599)
                     /locus_tag="PLU99_02585"
     CDS             complement(12021..12599)
                     /locus_tag="PLU99_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO15972.1"
                     /translation="MITESDIHGFFQSILERIGNAELRDKVVKTWVMACERGGWTSVD
                     ELQKMPFTLLTNCHGVDFIQHTIAVTEGAYGLAKAQEAAYAKLPYKIDYDRLLVGGLL
                     HDVGKLVETEKDGKGGYRKSRAGKCLRHPISGTALAHEAGIRDEAVLNTIACHAKEGE
                     GAPKCLETILIHQADFATFDPLVMMEKGNLIA"
     gene            13311..13559
                     /gene="acpP"
                     /locus_tag="PLU99_02590"
     CDS             13311..13559
                     /gene="acpP"
                     /locus_tag="PLU99_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO15973.1"
                     /translation="MAEIEAKVKKIVAEQMGVPESEISRDTSFVNDLNADSLDTVELV
                     MEFEDEFEMSIPDEEAEKIQTVGMAIDHIHKHLQTAGK"
     gene            13583..14818
                     /gene="fabF"
                     /locus_tag="PLU99_02595"
     CDS             13583..14818
                     /gene="fabF"
                     /locus_tag="PLU99_02595"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOO15974.1"
                     /translation="MHERRVVVSGMGAITPLGCSVSSFWDGLISSRCGIRTIEHFDCS
                     RFDVHFGGVCTDFDPAREIDPRRIKRMDRFAQMALIAARQAFIESGLQDNFDPVRVGV
                     ILGTGIGGLAEIEEQHLRLLQKGPGKVSAFTIPKLMANAGSATVSIEFGFQGVNATVS
                     TACASAGNAMADALRSLRRGDADIIFTGGTEATVTPLGIAAFAAMKALSTRNDDPEHA
                     SRPFDKDRDGFILSEGAGILVFEEYEHARKRGAPILCEVIGAGTTADAGDIVQPDPEG
                     RGGARAMAEALASARLNPEDVDYINAHGTSTMLGDIAETKAIKAVFGSRAYKVPVSST
                     KSAVGHLLGASGGVEAIASIMALRSNTLPPTINLEHPDPECDLDYVPNTARDARLQVV
                     MSNSFGFGGHNACVVFRKI"
     gene            complement(14870..15274)
                     /locus_tag="PLU99_02600"
     CDS             complement(14870..15274)
                     /locus_tag="PLU99_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO15975.1"
                     /translation="MPEPAPLILLVDDDLDFLEINRHILEPRGYRVACDTDPQAAWQR
                     LQTEKPNLIITDLMMANLDSGFSLARHVKQDPRLADVPIIMATAITSQAGLDFRPRTR
                     EDLAAMSIDAYFDKPIPPQKLLDKVAELLGRR"
     gene            complement(15468..16553)
                     /locus_tag="PLU99_02605"
     CDS             complement(15468..16553)
                     /locus_tag="PLU99_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOO15976.1"
                     /translation="MKIAAFEVESWERVAFEQLALDFDVRYTDRRLDLTNADDYADAE
                     VICTFIYSKLDKKVLSRFKDLRFICTRSTGFDHIDTAFCEAHNILVSNVPSYGDRTVA
                     EHVFGLLLTISHQLYDAIDRTRKGDFSLVGLRGFQLYGRTLGVIGAGNIGLHVIRIAR
                     GFGMRVLAYDVRPRPDLAVQIGFEYRPFEEVLAAADVLSLHVPDTPETRDMIGQEQFA
                     RMKQGVVLINTARGSIVNVQALMEALAAGKVRAAGLDVLPEEPMMREEAELLHRAIRT
                     EHDLETLLADHILLRQRNVFVTPHSAFCTEEAVQQILEVTRENIEAFARGAPQNVVIG
                     DPAALRGPAVPHDAGRAGEAEALPRRR"
     gene            complement(16657..17565)
                     /locus_tag="PLU99_02610"
     CDS             complement(16657..17565)
                     /locus_tag="PLU99_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO15977.1"
                     /translation="MAALSARQPRDEELLSPDFMTRLERLEILSRKIFIGRMKGERRS
                     KRKGESVEFADYRNYVVGDDLRFLDWNIYARLERLLLKLFMEEEDLNVSILFDVSKSM
                     DWGDPHKGLYVKRVAAALAYIGLVNYDRLSLYGYSSKLVHEMRGIRGRRLIGQVVPFL
                     RDLPFEGTSDFTAVGRHFALRHKGKGVVIVLSDFLDKGGYAGGLRYLLSRDLDIYVVQ
                     TLAPDEIDPTLVGDLRLRDVEDDDTAEVTISKPLLDKYKANLQAYCTELKEHCTRRGI
                     TYLFASTRVPFDVLVMSYLRQRGLLR"
     gene            complement(17661..18320)
                     /locus_tag="PLU99_02615"
     CDS             complement(17661..18320)
                     /locus_tag="PLU99_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOO15978.1"
                     /translation="MSRHQSQAHVAEAALDQSLIRQRKARPADFGLFLLKFLAKGRTI
                     SSAVPSSRSLVEGVLKLVDFGRPSTIVELGAGTGPVTEQIVERLGPQHRFVAVENDAD
                     FCAILRRRFPETALLEMDATLVAEPLARMGIHKVDYVLSGLPTPSLPRRSQINLWRWL
                     RSALGPNGLFIQITVAPLFFKPFYDRLFERVEYRMVWRNVPPGGVYYCSRPRQVLHLA
                     K"
     gene            complement(18522..20468)
                     /locus_tag="PLU99_02620"
     CDS             complement(18522..20468)
                     /locus_tag="PLU99_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO15979.1"
                     /translation="MSDTVLTPAAPVPSVLAQRQPAIPKARSRKAFWRMLALVRPHRR
                     RMTLGIVLGLGVAMTYAASLGGLLPVLRVIINDQTTFHQTLLKAASQEVVLADGTTAL
                     HWYAPVLTWAAQFFPDSLTPEAQMTTLLILISALVLINLVGNVLRCASTYFVLDASNR
                     MMMDLRRLMYRKALRVPVSQLSGDVSNRVSQFMSDAREMFAGVTTLFGKVAREPLKAI
                     CVLAVALYIDLRLTLLVLAVAPPAVLILWYYGRKIRKAVVRLLQGYGAMLGSLEESLQ
                     GIDVVKGYARENHERKRMWRLERQMLKQSLRLAWIEAISSPAIETVGVICTAAGVVWL
                     AWRQFHGDIEADHFVTMVALLAAMLDPVRKVANVYNVVQRAGAASSRIFEFLDQPEES
                     SPPQAKILPPNGARAVRFENLRFRYTPRQEPPALDGVTLDVRPGECLAIVGPNGSGKS
                     TLLKVLPRLLEPQEGRTTIDGIDVRELSLKNLREQIAVVTQRPVIFARSVRENIAYGN
                     ERASLDEIRAAARQAYAAEFIEQWPDGYETPLGEFGSALSGGQRQRIAIARAFLKPAS
                     ILIFDEATSEVDADSEHKIHAALSKLREGKTTFLIAHRHTVMEMADRLVVMDYGRIVD
                     VGTHSELIGRCPLYQALYRSPASH"
     gene            20936..22606
                     /gene="groL"
                     /locus_tag="PLU99_02625"
     CDS             20936..22606
                     /gene="groL"
                     /locus_tag="PLU99_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246509.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOO15980.1"
                     /translation="MAAKQMAFDQEAQKAILAGVEKLAAAVRTTFGPRGRNAVLDKGW
                     GAPTVTKDGVTVAEEVELHDKYENMGAKLVKEAASKTSDVAGDGTTTATVLAEAIYKE
                     GLRNVVAGFDPNGINRGIDAAVRAMVAELKNISRPVNLDPKRADEIVNIASISANNDR
                     AIGQIMADCFAKVGKDGVITVEEGKSLETTVDVVEGMQFDRGYLSPHFVTDPEAMECV
                     LEKAFVLIYEEKISSVTKLVPLLEKVAQAKRPLLIIAEDIEGEALATLVVNKLRGILQ
                     VCAVKAPGYGDRRKAMMQDIAILTGGKPIFKDLGIELDSVNIEDFGQAKRIRVDADNT
                     TIIEGAGSSADIKGRIEQIRKEIEDTTSDYDREKLQERLAKLAGGVAQIKVGAATESE
                     LKEKKARVEDALHATRAAIDEGIVPGGGVALLRAGKALETLKVKGDDEKVGVQIVKRA
                     ITAPIKQIAANAGYEPAVVLKRVSEKDGPYGFNAETGEYGDMMQMGIIDPAKVVRSAL
                     ENGASVARVLLSTACLIAEKPKEKKAGPGGPPGMGGMGDDDMGGMDMM"
     gene            22745..23059
                     /gene="groES"
                     /locus_tag="PLU99_02630"
     CDS             22745..23059
                     /gene="groES"
                     /locus_tag="PLU99_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOO15981.1"
                     /translation="MPTATAKKLKIRPLDDRVVVEPSEPEEKTAGGIVLPDTAREKPL
                     HGKVIAAGPGKLLEKSGERGKMSVKVGDTVIYGKYSGTEVELEGEKYVILRESDILAI
                     QE"
     gene            23091..23543
                     /locus_tag="PLU99_02635"
     CDS             23091..23543
                     /locus_tag="PLU99_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15982.1"
                     /translation="MAEALREFRAETYAVAAQEHVTAAVELHEAERFALAHYIAGLAV
                     ECVLRAFKSRLDPVLDERHDLYQLAKGGRFFDRVSEKHLPDLLAAFGEVVARWDNGHR
                     YRSESALRKFLVQRRLFSGKGDLLRNSSRRIVNAALQVVSFGAQTWKN"
     gene            23531..>23721
                     /locus_tag="PLU99_02640"
     CDS             23531..>23721
                     /locus_tag="PLU99_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15983.1"
                     /translation="MEELIKRLRQLLERVFPGATVELEVAAPEKVGGFLIWNGFDQVD
                     QVERQRRLWTAIRGELSRE"
BASE COUNT         4418 a         7532 c         7310 g         4361 t
ORIGIN      
        1 agcgagcacc ccgacctggc tgacctgatc gcgcctaccg ccctggacgt gaccgaccgg
       61 gccgcggtcg aacgcgtgat tgaggaaacg ctcgaacgcc acaaccggct ggacattctc
      121 gtcaacaacg ccggcatcac cgccgacggc ctggtaatgg gcatgaagga cgagcagttc
      181 gatagcgtgc tggacaccaa tctgcgggcc gcattctggg ctattcgagc ggtctgccgg
      241 ccgatgattc gcgcccgccg cggccgcatc atcaacatcg ccagcgtgtc cggcataatg
      301 gggaatgaag gccaggcgaa ctatgccgcg gcgaaggcgg gcctgatcgg cttgaccaag
      361 tcggtcgcga aggagatcgc caagcgcggc gtgacgtgca acgcggtagc gccggggttc
      421 atcgaaacgg acatgaccaa tgtgctgccg gaagagttga aggcccgcgt gaagcaggtg
      481 gtcccaatgc agcgttttgg gcgtccggaa gaagtggcgg gcatggtcgc gttcttagcg
      541 ggcccggcgg ccagttacat cacggggcag gtgttcgtgg tcgacggcgg gctgcatacg
      601 taggtcagaa agcgaccggg cgcaccacgc acatacgacg gattgccaac tgggcccgca
      661 ctgcttgaag ctgcgctttc aagcagtggc acacgtgaga gatgcacaca cgagttgcac
      721 acgcgagaga tgctcacgcg aagatgcata cgcgagagat gcacatgccc gactcgcctc
      781 tgctgctagg tattgacgtc ggtaccaccg gcgctaaggc cattctcatc actgccgccg
      841 gcgccgttct ggccgaagct accagcgaat accccatggc gacgcggcgg ccgctgtggg
      901 cggaacagaa tccccatgac tggtggagcg cgacggttgc cagcgtgcgt gaggtgctgc
      961 accaggcggc ggcgcccgcg gaggcggtag ccggggtcgg cttgaccggg caaatgcacg
     1021 ggctggtgct gctcgacgag cacggcgagg tgttgcgccc ctgcatcatg tggaacgatc
     1081 agcgaaccgg gccgcagtgc gccgcgatta ccgggcaagt gggcgaacag cggcttctgc
     1141 agttaaccgg gaatcccgtg ctgccgggct ttacggcgcc gaaaatcgtc tgggtacgcg
     1201 agaatgagcc ggaggtctac gggcggatca agcacgtgct gctgcccaag gattacatcc
     1261 gttaccggct cacgggcgag ttcttcagcg acgtgtcgga cgcctcgggc acggccatgt
     1321 tcgacgtcgg ggggaggtgc tggtctgagg agatgttggc cgcgttggat gtgccccaag
     1381 agtggttgcc ggaggttacc gaatcgctgg tggccagcgc ggccgtaagc gcggcggcgg
     1441 ctgcggaaac gggtctgaag gcgggtacgc cggtcgtcgg cggagcgggc gaccaggcag
     1501 cgcaagccgt gggcacgggc gttgtatccg aggggatagt ttcggtgacc ataggcacgt
     1561 cgggcgtggt gttcgcagcg cgcaacgctt atcgcccgga gccgcgcggc cgattgcatg
     1621 ccttttgcca cgccgccccg gggctgtggc acctgatggg cgtaatgctg gcggcgggcg
     1681 ggagcttccg gtggtatcgc gacacgctgg gggaggtgga gcgctcgcag gccgcggccg
     1741 gcggcggcga tgcgtacgac atcctgaccg ctgaggcggc ccaggcgcca gccggctgcg
     1801 aggggctgct gttcctgccg tacttgtgcg gcgagcggac gccgcacccg gatccgcatg
     1861 cacgcggggc attcgtgggt ctgacgctgc ggcatacgaa ggcgcatttg acacgggcgg
     1921 tcatggaagg cgtcaccttc ggcctgcgag attcgttgga gctgatgcgc gggctgggcc
     1981 tggaaatccg gcaggttcgc gcttccggcg gcggagcccg tagcccggta tggcgccaga
     2041 tgctggctga cgcattcggc acggaaatcg tcaccgtgaa catgacccag ggggccgcct
     2101 tcggcgcggc actgctggcc ggtgtgggga ctggtgtgta cgccgacgtg ccgagcgctt
     2161 gccaagcggt cgtcaaggtt agcgagcgct gcgcaccagg accggacgag gctgtgtacg
     2221 cacggtacta ccggcgttat caggcgctgt atccggcgct ggcgcaggaa ttcgcggcgc
     2281 tcgccgaagc agaacggtag ccgccgcttc gaggcacgac ggcgagcccg gcttaaccgg
     2341 ggcgcaccat ctaccgcttc gcagtcctcg gcgtcttgcc tgcaagatac tgatcaatcg
     2401 agcgggccgc tttcttgccg tcgcccatgg caagaatcac ggtcgcggag ccgcgggtga
     2461 tgtcgccgcc cgcgtaaacg cccggcttgc tcgtggcgcc gttttcgtcc acctcgatgt
     2521 agccccactt gttcgtcttc aggtcaggcg tggttgaggt cagcagtggg ttagcgcgcg
     2581 tgccgatggc cggaatcgca acgtcgcact cgatcacgaa ctcggagccc ttgaccggca
     2641 caggccgacg gcggccggac tcgtccggct cgcccagctc catctgctgg cactcaacgc
     2701 cgcggatcca gccgtcctcc gtaccgaggt agcgaaccgg gttggtcaag aacttgaaga
     2761 tgatgccttc ctcttccgcg tgcttgacct cttcgacgcg cgccggcatt tcgttgcggc
     2821 tgcggcgata aacgagggtg gcctccttgg cgcccaggcg cttggcggtg cgcacggcgt
     2881 ccatcgcgac gttgccgccg ccgatgacga ccacgcgctc gccgcggatg atcggtgtgt
     2941 cgaagtcagg gaagtcgtaa gcccgcatca ggttggaacg ggtgaggtat tcgttcgccg
     3001 agtagacgcc cttgaggttc tccccgggga tgttcataaa gatcggcagc ccggcgccat
     3061 tggcgatgaa catggcgtcg aacccctctt cgttcagcag ctcatcgacc gtaaacgtgc
     3121 gcccgatgac gacgttgcag atgatcttaa cgcccatttg ccgcaggcga tccacctcgg
     3181 cgtcgatgat cttgttcggc aagcggaact cagggatacc gtacgtcagg acgccgcccg
     3241 gctgatgcag ggcttcaaaa accgtgacat tgtggccttt gcgggccaac tcgccggcgc
     3301 aggtcagccc ggccggtccg gagccgacga ccgccacgcg gaaaccggtg ggcgggccga
     3361 tcagttgctt catatccagg ttttcacggg cccagtcggc gacgaagcgc tccagatgcc
     3421 ccacggcgac cggttcgccc ttcttgccgc ggatgcagag ctgctcgcac tgctcctcct
     3481 gcgggcagac gcggccggtg atgccgggca gcgcgttggc gctgaggagc agctcggcgg
     3541 cgccgcgcaa gtcgccggcg gagactttct cgaggaagcc gggaatatcg atgccgacgg
     3601 gacagccgct gacgcacttg cggtctttgc attgcaggca gcggttagcc tcaagttggg
     3661 cgagggccac ggaaaagccc aggttaacct cgcggaaatc gcgattgcgc tccatgggct
     3721 cgcgctccgg catgtgctgc cggctgattg ccatacgctc tttggcggtc atcgacatgg
     3781 gactcaacct caaaatcatg ccaggattaa tgacccggca tagctctcta aaccgcgacg
     3841 cggtgttaag ttctcaagcg tccggcctgc ggacccgacc tccaaaatcg ccgaccggcg
     3901 ccgccttcca tttagcctca gcggcttacc ttggccggtt cattgagccg gcactggtgg
     3961 ccggcgtgct ctgcgcgctc cagcgaaatc tgttcctgct tgcgatacgt ggtcagtcgg
     4021 tccgacagct cggcgaagtt gaccaggtgg ccgtcgaact ccggcccgtc cacgcacgcg
     4081 tacttggtct tgtcgccgac cgtcacgcga cagccgccgc acatgccggt gccgtcgatc
     4141 atgatcgggt tcagggacac gatcgtcttg atccccgccg agcgggtcag ctcgcagacc
     4201 gcgcgcatca tcggcaccgg accggccgca tagacgaccc cgatcggccg ccgcttgtcc
     4261 gcgatcagct cacgcaactt gtcggccacc gtgcccttga agccgtagct gccgtcgtcc
     4321 gtcgtcgcgt agaccacgtt cgaaacccgg cgcagctcgt cctcaagcac gaccagctct
     4381 ttcgttcggc cgcccgtaat tgatgatacg aagcatccgg cctcccgcag agcggtgacc
     4441 aacgggtaaa tcacggccgt gccgacgccg cccccaacca ggactgcgtg atcgacacgc
     4501 atgatctccg tgggacggcc gagcgggccc atgacgtcgg cgatcgtgtc gccggcctgc
     4561 ttccggcaca gctcacgcgt ggtcttgccg atggccttca cgatcagctc aatcgtgccg
     4621 gtgttccggt cggcgttggc catggtcagc gggatacgct cgccggtttc gtgcaggcgc
     4681 aaaatgacga actggcccgg ctgccggtgt ttggccacta acggggcctc gatgcgaaac
     4741 catttcacgt ccgatgcgag ctgcctggca tccaaaatct tgaacatgta ccagctccgt
     4801 ggcgggtggc attaggacaa gaccgaaccc ggtcccaccc aacctcgccg ttgcaaatgc
     4861 cgtaccagtg ttggtccggc cagttagaga tcgtgccaag cccgcaatac ggccaatatt
     4921 ccgaatatgc ccgccggtgc gaaaatttcc gcaatcgggg gcaaacatga attagtttca
     4981 tgctggcagt gaaaatcgat ctccactaaa cgccgacgcg ttcttggcgg tcggcaggct
     5041 gttcttcagg ctccccttcg cgctcggccc cggactgggc atccgcacga ccatccgggc
     5101 ggacaatgta aatcgggcgc tgcttaacct cctcgtacag ccgcccgacg tactctccca
     5161 gaatccacat tacggtcaga ttcacgccac ccagaaccag aacggcggct agcgtcgatg
     5221 cccacccggc gacggtccga tccgtgaaga agtatgctcc cagcacgaac agcagataga
     5281 acgtcgccac gaccgaaacg cacgccccag ccaaacccgc cagccgcaag ggcacggacg
     5341 aaaacgaaaa aatcgcgtcg cccgccagcc gaatcatggc ggaccatgag tacttggagc
     5401 gcccggcgtg ccgcgtggcg gcctcgtaca gaatttcgtc ccgcgtaaag ccgatccagt
     5461 gcaccagacc gcggttgaag cgacaacgct cggggcaggc taggaatgcg tccaccgccg
     5521 ggcgagacat cagccggaaa tccgaagctc cgggcgtgac cgccagccgc gataccgctg
     5581 acagcagccg gtagaacgcg cgcgacccgg cccgtttcag ggggctgtcg tccaccgggg
     5641 cgcgtcgcac ggcttggacg acgtctgcgc cggcatccca ggcggctacg agttgaggga
     5701 tcagttccgg cggatgctgg agatcgccgt ccatggtgat gacggccgca ccgcgcgcct
     5761 gcgccaatcc ggccaccaaa gcagcctgat gtccgaagtt tcgcgagagc cgcaccagtc
     5821 gcacagccgg gcaatcgacg gcggcggcgg tgattcggct ggcggtttca tcgcaactgc
     5881 catcgtccac gaacaagagc tcaaaatcgc ggttcaacgc ctgcagaacg cggagagtac
     5941 ggtccagaaa ggccgcaacg ttcgcggctt cgttgtacac cgggacgacg acggacagaa
     6001 ttgggcgaaa ccgggccgaa cgcatttgat ccatgttacg gcgtcggccg atttcgtgca
     6061 gcccgggcgt aaattttctt gttgactctc ttgacagaag tgcccatagg tccggattat
     6121 gttttttggt tgggtttgcg cggtcctgcc attgtggcgg gaaccaaccc ttttttcagt
     6181 ctcgcagcaa tggagaagcg cgatggattt ttggggattg ttgatctgga tcggaatcgg
     6241 ggcactcgtg ttctggctgt tcacgcaggc cgtctaacag gaatccgctg gcgcggatga
     6301 gaagcatggc ccccgccggt ttgcgggggc cgctttgacc tgcctttttt tcgaaggagt
     6361 tcaaatgttt ggtggatgga ttgagttgct gttctggggc gcaatcgccg cggtcattat
     6421 tttcattctc accggccagg cggctgccta ggtgtagaag tgctggtacg tcgcggttgc
     6481 gactttatcc agtgacaagc gacttctgaa cgagctcccc atgcatgtgg ggagcctaat
     6541 gtgaaggagg acagcatggt aggatggtgg tggtggctga cttgggccgg cgtcatccta
     6601 ttcgttcttg ccctgctcgc cggtcagaat gtctgattta ccgaggtgat ttcaaaaggg
     6661 ctcgcgggcc caatgccccg tgagcccttt gtgaggtgtg cttatgactt ggttgctggg
     6721 tttgttgttt ttcggcggca tcggcctgat tctcgggctt ctgatcccgt cgttgatccc
     6781 ggcttagtct acaagcagtg ctcttacgct gattcgagcc ccgcagccgc ggggctctcc
     6841 cgaacgcgcc gatcctctct gaaccggaga gtatcggcgc gtttcatttg agcgagcctg
     6901 cccaggcgtg cacctcagaa cccgcgcggc cggctcggat agccgcggac tccgcttagt
     6961 tctttccgcg ctcgttctcc aagcccatgc cgctgcggtc cggcggcgga ggcggcggag
     7021 gttcaatgaa cgggtgcagc gcaatttctt cgagcatcag ctcgatcgca cgatcaagct
     7081 gcgggtcgcc gccgtcgacc atccttgacg ggtcgtcgat cacctcgatg tccggatcaa
     7141 cgccgtgccc ctcgatggcc caggtgctgt cgaggttgta cgcgccgaac gtgggcacgc
     7201 tcatgtgccg cccgtcgatg aaatcggggt tgcccgaaat cccgaccaat ccgccccaag
     7261 tccgcgtgcc gataagcttg cccagaccgg tctgccgaaa gtacgccgga aaagcatctc
     7321 cacccgagcc agccaagccg tttatcagca tgcacttcgg gccttgatgg ctttgtggcg
     7381 gcgtgcggcc cgggcgcccg tggcgccgcg cccacatgct caagagcggg cgattcaaca
     7441 actcgatgaa ccgatccgga atctggccgc cgccgttcca gcggtcgtcg atgatcagcg
     7501 cctgcttgaa gcgttgaccg tggaattgac gcaccaaatc ggcttgcccg tcgcgcccgg
     7561 tgttcggaac gtagatgtag ccgactttac ccccgctgcg ctcatccaca taagcccgtt
     7621 tggcctcgat ccaggcgcga tagcggagct cgcgatcgct gaccagcaac tcgacgacga
     7681 catcacgcgc ctcgtcattc agggcaggct ggtcgctgac ggtcaagaca accgtgcggc
     7741 ccgccaaacc ctggaaagcg gcccacggat ctgcccgcgt atcgagcggc aagccgttca
     7801 ccgccaatag gtagtttccc tccctgacgt taatgcccgg ctggctcagc ggtccgcggg
     7861 catcgaggtc ccacggcccg ccggcgtaaa tcttgctgat tcgataggcg ccatcctgca
     7921 gctcgaaatc gaccccgagc aaccccaccg aaacgcgcgg cccctggtcg ggatccgtag
     7981 cgttgtaata ggcgtgcccg acgttcaact ccgaaatcat ttccttgatg atgaaactca
     8041 cgtcctcgcg gcaaacgcaa tcctcgagca tcttgccgta tcgtgaccgg atgcccggcc
     8101 agtcaacgcc gtgcatgttg ggcacgtaga agaaatcgcg catgatccgc caggcctcgc
     8161 tgaaaacttc gcgccactcc tgccgcggat cgatgctcac caccatgccg ctggtgggca
     8221 tgggcttgtc ggtcttctga tccggccgag ccttgacgat gaacagcttg ccgtccttgc
     8281 gaacgaggat ttttttaccg tcggcgctga ggtcgaagcc gcgcacgtcc ttgagcacgg
     8341 tcttctcttt cttctcgtcg tccttgacgt cgaagatttg gatggccggc ttgtcgtccg
     8401 aaccgcgcaa cggatgacgc acgtacagca aatgcccctc atcgttgact gcgaggtggc
     8461 tgaaattgcc ggccggcacc ggcaatggaa cggcccgcgc ctcgaacccc tcgagctcga
     8521 tttctacggg cttgatcttc tttttcttct tgtctttctt gcttactgct tcccgctctt
     8581 cggcttcctg gggctgttcg gccggcgcgc tggaggtcgc cggctgcccg gcgtcgccnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac atatacccag gtgttgccca
     8761 gctcagtgta gatcggcgta tcgacgtttc gattcgtgat gaagtagagg taatcgccct
     8821 ctcgatcgaa tgtcgggttg ctgctgttga agagtgcgct cgtaagctgc tgcttttcgc
     8881 cgctctccgc gttgtacagc cagatcgtgc tcagatcgct gtcggtccgt ttagtgtacg
     8941 cgatccagcg cgaatccggc gaccaactcg cctcgggcac gcgggcatcc gggtcggtgt
     9001 ccaccaactt cgattcgtcc tcttcgatcg cccgcaggta caggttgccg gcgttgtcgc
     9061 cgtagaccac gtacttcgaa tccggcgacc agagaatgcc gtagcggaag cccggcccgt
     9121 gattcgtcag cttgcgcggc tcggacttgc cgtcggattg caccaggtac agctcatttt
     9181 cgtcggtggc gtccgaccag tacgcgatcc agcgcccgtc cgggctccag ctcggatagc
     9241 gctcatgcac gcccacgctg cgcgtcagat tccgcggcga gccgtctcgc gccggagccg
     9301 tccagatgtc gccgcgcgct tccatcacgg cccgcttacc gctcggcgaa attgtgacgc
     9361 tgcggatgtc gctgctcacg tcgatgagct gtggtcgaat cttcggccga tcgccgggaa
     9421 tcgtgacgtc gaccgcgcgg ctctggccgc ttgccaggtc gagcagatac agatccgtgc
     9481 cgttctgaaa aacaatctcg ccctcaccgt tcgggccggg cccgatcgcc ggccacttga
     9541 tgtcgaactc cgcgaagttc gtgatctgcc ggcgttcttt gctgacccgg tcgtacgacc
     9601 agatgttgag gcggtgttcc ggtcccgcgt cggacatgta gtaaagcatg ttgccgtgcc
     9661 acatcggctg cgagtcggtc cccgcccagc aggtgatgca ctcggcgcgg tggtccttca
     9721 ggttgaacag ccagatgtcg gtcgccatgc cgccgcaata acgcttccag gtgcggttat
     9781 ccgtggtgtg cagggtgtac gccagccact cgccgtcggg gctgatggcc gcgttcgctc
     9841 catagggcac gggcagtttc tcgggcaaac cgccgctggg tgcgaccgtg aagagttgtg
     9901 tcatgcgcgc gaagggcgct tcggcgttcg agaagaatat cagccggccg tccggagaaa
     9961 agtcgcacaa attctcggtc gccgggtggt gcgtcacacg aaagggcacg ccaccggtca
    10021 ccggcaacgt gtacagatcg gtattgccgt catagttagc catgaacgcg attgtctgcc
    10081 cgtcgctgct gaagcgcgga aacgactcca cgccggccgg actggataac ggatgggcca
    10141 cgccgccgtc gcgcggcacg acccacaggt cattcgcgta tgaaaatacg atgtgcgtgg
    10201 cacttacgtc cgggttgacg agcatcccca cgtgtggccg cacgtccccg cgcgcggcgt
    10261 acatccccag ccccgccgtc caaaccagcg ctaccacaag cgacatcccc cgcaggctgc
    10321 acccgcgcat atctcgtcct cttacaaaac gccgggtctg cggaccctac ttacacgacc
    10381 ttgcacaagc ccccgtacct taacgaaaaa atgctggagc gccaccgcct cgacaaatct
    10441 cgacaggcgg catttatacg gacttcgcgg acggcgcgta ctgacccccg tgccccctgc
    10501 ctctcttcta tctccctact ggatctagaa ccaccacgcc gcgccgggct gctccttaat
    10561 gagcacgccc cgcaaatacg ccaccgcctt ctcgaagccc tcctggacgc tcatgtacgc
    10621 gtcctcgtgc tctatgctga ccactccgtc gtagccgacg cgcttcagca tgctgatgaa
    10681 cggcttccag aactcgtccc cgtggccata gccgcaggta cggaagaccc aacttcgggc
    10741 cttcagatcg ccgtaggatt tggcatccag cgtaccgttg atgcgcgtgt tgtgcgggtc
    10801 gacgccggtg tctttgccat gcacgtggaa caagcagccg gcttcgccaa aaacgcgcgc
    10861 cgcttcgatc ggatcgatgc cctgccagaa aaagtgcgac gggtccagat tggcccccag
    10921 gctcggcccg caagcatcgc gcaagcgcaa caaagtgtcc gggttgtaga cgcagaatcc
    10981 ggggtgcgcc tcaaacgcga tcttcacccc ttcatccgcc gcctcccggg ccttcttttt
    11041 ccagaagggc accaacacct cggtccactg atactcctga atcttcgtga aatctggcgg
    11101 ccacgcggcg gtgacccagt taggggtctt atcgccgggc gccccacccg ggcagccact
    11161 gaaggtgatg accacgtccg tgcccagttt cggtgccagg cgaacggccg tctcatgcgc
    11221 cttcaaatgc tgctgcgcaa tggccgcatc cgggtgaatc gggttgccgt gcacggccaa
    11281 gccgatgatc gccagcccgc gccgagcgca gtcgtctttt atcttctgtt gtgcggccgg
    11341 ttcgcgcagc agttgctcgg gatcgaagaa cggtgtgccc ggatagcccc ctaccggcag
    11401 ctcgatcgct tccaacccca gcgtcttgca gtaatccagc gccttttccc accccaggct
    11461 ggccagtccc gcccccatta cgcccagttt catcggatgc tcctcgtcct cgagtaattg
    11521 attgtcagtc ttcacctggc cagcggcttg gcaggcttct tctcctatcc tcccccttgg
    11581 agctactgcg ttggccgggt tcaggaatcc ggctcgcagg tcacgttcag tttgcagctc
    11641 gcgaactgct cgacatacag ctcggcccgc tctttgtgcg tgaccagcaa catgctcgag
    11701 cccgtctcgt gcgcttcaag agtgcgtgcc acggcttctt ccgtgttcag cggcgtgagc
    11761 ttcacgatcg tgcgcacgac gtggtcgacg gtatttacgt cgtcgttatg cagaattacc
    11821 cggaacggcg gtagccgccg cgttttctgg tcttgctcga tggtcggttt agtgctctcg
    11881 accaagatgc tctcccgggc tgcgggcgag tgcgccaggc ggaatcgccc ggcattacct
    11941 tcaagtgcaa tgtcggcccg gaaccggccg catgcctagt gaggttatta tatcctcacc
    12001 caaatgaact caagtcccgc tcacgcaatc agattcccct tctccatcat caccaatggg
    12061 tcaaaggttg cgaaatccgc ctggtgaata aggatcgtct ccagacattt cggcgcgcct
    12121 tcgccctcct tcgcgtggca ggcgatcgta ttcaaaaccg cctcgtcgcg aatcccggct
    12181 tcatgcgcca aggccgtccc cgaaattgga tgccgcaggc acttcccggc ccggctcttg
    12241 cgataaccgc cctttccatc tttctcggtc tcgaccagct tgcccacgtc gtgcaacagc
    12301 ccacccacca gcaaccggtc gtaatcgatt ttgtacggca gcttcgcgta agccgcctcc
    12361 tgggctttgg ccagcccgta agcgccttcg gtgacggcga ttgtatgctg aatgaaatcg
    12421 acgccatggc aattcgtcag gagcgtaaac ggcatcttct ggagctcgtc cacactggtc
    12481 cacccgccgc gttcgcaggc catcacccag gtcttgacga ccttgtcgcg cagctcggcg
    12541 ttgccaatgc gttcaagaat cgattgaaaa aagccgtgaa tatcggattc ggtaatcaat
    12601 tgtcgctcct tccgtacggt cagggtggta tgctcggagt cacttccggg ttatttcagc
    12661 gcaagtccat ggcctgaact cttccggcgc gtcaggcgca tgccggatcg cactccatgg
    12721 ccattttgcc accaggccgc tcgccgagca aacaccgcac gccggtagcc gccgcttacg
    12781 tccacctccg gtggcgcagc aagatcggcc ctttactgca tgcgcttccc cgctttgcag
    12841 caagatcggc cctttactgc atgcgcttcc ccgctttttt gaaatcaacg atcggtgaaa
    12901 cgtgcttttt tggtgaacag gccgttgccg ccctcagccg cccgttacca actaatttcc
    12961 gccagccccg cgcatgtccc ggcccgccga gcaccagcgg caacctgccg ccgtcaaggg
    13021 gtcccaaaac cgggtcccga ccggtcaaaa aaacctcccc cttgggcccc tggtaggagt
    13081 gacagacctg cccttggccc ccttggcaag ttcgccgcac cggtttaata tcccgctccg
    13141 ttgcggcgat cgcatcgcga ccgccgcgcc tacaatcttg ccgccttagt tcggcggcgt
    13201 gcacgggcag cccggcttta tggtgccgcc agcgcctcag cttggtgtcc cgtacatcca
    13261 gggttgcttc ggagtcccag tattctggcg gaggaaccgg cgtgccggaa attgcagaaa
    13321 tcgaagccaa ggtcaagaag atcgtggccg aacagatggg ggtcccggaa agcgagatca
    13381 gccgggacac ttcatttgtc aatgatttga atgccgactc gctcgacacg gtcgagctgg
    13441 tcatggaatt cgaggacgag ttcgagatga gcatccccga cgaggaagcg gagaaaatcc
    13501 agaccgtcgg gatggccatc gatcacattc acaagcacct gcaaaccgcc ggcaaatagc
    13561 gcctggctca acgggtcgtc cgatgcacga gcgacgcgtc gtcgtcagcg gaatgggtgc
    13621 cattaccccg ctgggatgct ctgtctccag tttctgggac ggtctcatct ccagccggtg
    13681 cggcatccgt accatcgagc actttgactg cagccgtttt gacgtccatt tcggcggcgt
    13741 ctgtaccgac tttgatcccg cccgcgagat cgacccgcgg cgcatcaaac gtatggaccg
    13801 cttcgcccaa atggccctta tcgccgcccg ccaggccttc atcgaatccg gcctgcagga
    13861 caacttcgac ccggtgcggg tcggcgttat cctcggaacc ggaatcggcg gcttggcaga
    13921 aatcgaggaa cagcacctgc ggctgctgca aaaaggccct ggcaaagtct cggccttcac
    13981 cattcccaag ctgatggcca acgccggctc ggccaccgtc tcgattgagt tcggttttca
    14041 gggcgtcaac gccaccgtct cgaccgcgtg cgcctcggcc ggcaatgcca tggctgatgc
    14101 cttgcgcagt ctgcggcgtg gcgatgcgga catcatcttt accggtggca ccgaggcgac
    14161 cgtcacgccc ctgggcatcg ccgctttcgc cgccatgaag gccctcagca cgcgcaacga
    14221 cgacccggag cacgcctcgc ggccgtttga caaggaccgc gacggattca ttctcagcga
    14281 gggcgccggc atactggtct tcgaggagta cgagcatgct cgcaaacgcg gcgcgcccat
    14341 tctctgcgaa gtcatcggcg ccggaacgac ggccgacgcc ggcgacatcg tccagcctga
    14401 tcccgagggc cgtggcgggg cccgggccat ggccgaagct ctggccagcg cccgcctgaa
    14461 cccggaagac gttgactaca tcaacgccca cggcacctcc acgatgctcg gcgacattgc
    14521 tgaaaccaag gcgatcaagg cggtgttcgg gagccgtgcc tataaagtgc cggtcagcag
    14581 caccaagagt gccgtaggcc acttgctagg cgccagcggt ggcgtggagg ccatcgcttc
    14641 gatcatggcg ctgcgcagca acacgctgcc cccgacgatc aacctcgagc accccgatcc
    14701 ggagtgcgac ctggactacg ttcccaacac cgcccgcgac gcgcgcttgc aggtggtaat
    14761 gagcaactcc ttcggttttg gcggacataa cgcctgcgta gttttccgga agatttgaaa
    14821 cagcagctcg gcgcatagcc cggccggccg tcggtgacgg acatccgggc tatcgccgcc
    14881 ccagcaattc cgccaccttg tccagcagct tctgcggcgg gatgggtttg tcgaagtacg
    14941 cgtcgatcga catggcggcc aagtcttccc gcgtgcgcgg gcggaagtcc aggccggcct
    15001 gactcgtgat agcggtggcc atgatgatcg gcacgtctgc cagacgcggg tcttgcttta
    15061 cgtgccgcgc cagcgagaaa ccggagtcca ggttcgccat catcaagtcc gtgatgatca
    15121 ggtttggctt ttcggtttgc aggcgctgcc aggccgcctg cgggtcggta tcgcacgcta
    15181 cgcgataacc tctcggctcc aggatgtggc gattgatctc gaggaaatcg agatcgtcat
    15241 cgaccagcag gatgagcgga gcgggttcag gcacctatgg ctccggacat tagatcagac
    15301 ctcgtttcgg cgcgccggtc gccacggcgg ctcgccgcac gcgagatcgc gtcgttccat
    15361 acgccactgg cgtgttttac cgcgcccgcg gcagcctgca aacgccgccg gtgcggacaa
    15421 agtccgcaag caccgctgac gccggatcgc cggggatgcc gctccggcta cctacgccta
    15481 ggcagagcct cagcctcccc cgcccggccg gcatcgtgag gcacggccgg gccacggagg
    15541 gcggccgggt cgccgatgac cacgttctgc ggcgcgccgc gggcgaacgc ctcgatgttc
    15601 tcgcgcgtca cctcgagaat ttgctgcacc gcctcctccg tgcaaaacgc gctgtggggc
    15661 gtgacgaaca cgtttcgctg ccgcagcagg atgtggtcgg cgagcagtgt ctccagatcg
    15721 tgctcggttc gtatcgcccg gtgcaacagc tccgcttctt cgcgcatcat tggctcttcc
    15781 ggcagcacgt ccaagccggc cgcccggacc ttgcccgccg ccaacgcttc catgagcgcc
    15841 tgcacgttca cgatgctgcc gcgggccgtg ttgatcagca ccactccttg cttcatccgc
    15901 gcgaactgct cctgcccgat catgtcccga gtctcaggcg tgtccggaac gtgcaggctc
    15961 agcacgtcgg ccgctgcgag cacttcctcg aatgggcggt attcaaaacc tatttgcacg
    16021 gccaaatccg gtcgcggccg cacgtcatag gcgagcaccc gcatgccaaa gccgcgggcg
    16081 atacgtatca cgtgcagtcc gatgttgccg gcgccgatca cgcccagcgt gcgaccgtag
    16141 agttggaacc cgcgtagccc gaccagcgag aagtcgcctt tgcgcgtgcg gtcgatggcg
    16201 tcatagagct ggtgactgat cgtcagcaac aggccaaaaa cgtgctcggc gacagtccgg
    16261 tcaccgtagc tgggcacgtt gctgaccaga atattgtgag cctcgcagaa ggccgtatcg
    16321 atgtgatcga aaccggtgga acgggtacag atgaatcgca ggtctttgaa ccggctgaga
    16381 actttcttgt ccagcttgga atagatgaac gtgcagatga cctcggcgtc agcgtaatcg
    16441 tcggcgttgg ttagatcgag gcggcgatcc gtatagcgca cgtcgaaatc cagcgccagt
    16501 tgctcgaaag cgacgcgttc ccacgactcc acttcgaatg cggcgatttt cacgtgcgtc
    16561 cccgacaaat attttcacgg cgaatttgta gcttaggccc ccgggtttgc gacgaagtaa
    16621 caccgggatg aaatgcccgt aagtcaacgc ccgccgctag cgtagcagcc cgcgttgccg
    16681 caagtacgac atcaccagca cgtcaaacgg cacgcgcgtg ctggcaaaga ggtaagtgat
    16741 gccccgccgc gtgcagtgct ctttcaactc agtgcagtaa gcctggagat tggctttgta
    16801 cttgtcgagc agcggcttgc tgatggtgac ctcggccgtg tcgtcgtcct ccacgtcgcg
    16861 caggcgcagg tcgcccacca gcgtcgggtc gatttcgtcg ggcgccaacg tttgcacaac
    16921 gtagatatcc agatcgcgtg agagcaggta gcgcaagccg cccgcgtacc cgcctttgtc
    16981 gagaaaatcg ctcaacacga tgaccacgcc cttgcccttg tggcgcaggg cgaaatgccg
    17041 gccgaccgct gtaaagtccg acgtgccctc gaacggcaga tcgcgcagga acggaacgac
    17101 ctggccgatc aggcgtcgcc cgcggatgcc gcgcatttcg tgtaccagct tgctggagta
    17161 gccgtacagg ctgagccggt cgtagttaac tagcccgatg tacgccaggg ccgccgcgac
    17221 ccgcttgacg tacagcccct tatgggggtc gccccagtcc atgctcttgg aaacgtcgaa
    17281 caggatgctg acgttgaggt cctcttcctc catgaagagc ttgagcagca ggcgctcgag
    17341 gcgggcatag atgttccaat ccaggaaacg aaggtcgtcg cctacgacgt agttgcggta
    17401 atcggcgaat tcgaccgact cccccttccg cttgctgcgg cgctcaccct tcatgcgccc
    17461 aatgaagatc ttgcgcgaga gaatctcgag gcgctccagg cgtgtcatga agtcaggcga
    17521 gagcagttcc tcgtcgcgcg gttgtcgggc ggacagtgcg gccaaagcga tcctcaatta
    17581 ccgaaccgcc cggcattgta acaattgtgt gcgggccggg agtagcgcct cgcccggact
    17641 aaattgcgcg gcgcgccacc ttatttcgcc aggtgcaaaa cctgccgcgg ccgcgagcag
    17701 taatacacgc cgccgggcgg gacattccgc cagaccatgc gatactcgac acgttcgaac
    17761 agccggtcat aaaacggctt gaaaaacagc ggcgcgaccg tgatctggat gaacaggccg
    17821 ttgggtccca gcgcactgcg cagccagcgc cacaggttaa tctgggagcg cctcggcagt
    17881 gacggcgtcg gcagcccgct caggacgtaa tccaccttgt gaattcccat ccgtgccagc
    17941 ggctccgcca cgagcgtcgc gtccatctcc agcagcgcgg tctcaggaaa gcggcggcga
    18001 agaatcgcgc aaaagtcggc atcgttctcg accgcaacga accggtgctg cggccccaaa
    18061 cgttcaacta tctgctccgt cacaggaccg gtaccggccc ccagctccac gatcgtgctc
    18121 ggccgcccga agtcaaccag cttcagaacg ccctccacca gcgagcggct tgacggaacc
    18181 gcagacgaaa tggtccgccc cttggccaag aacttcagca ggaacagccc aaaatccgcc
    18241 ggccgggcct tacgctgtcg aattagcgac tggtccaacg ccgcttcggc tacatgcgcc
    18301 tgcgactgat gtctgctcac gtgttctcca ctcgctcgaa tggctgtccg gcaagctcgc
    18361 gcctgagccg ccattggccc cgctcggcta ccgaccggga accacgccgc aggcccggcg
    18421 accgccgtcg gctcccacgg tgatgggcgt acatcatagc gtctcgctgg agtctatggt
    18481 agtacggcta aaccgaattc gggggatggt cgtcccggtc ctcagtgcga ggcgggggat
    18541 cggtacagag cctgatagag cgggcaacgc ccgatcagct cggaatgcgt cccgacgtca
    18601 acaatgcggc cgtaatccat cactaccagc cggtcggcca tttccattac cgtgtggcga
    18661 tgagcgatca aaaaagtcgt cttaccctcg cgcaatttgc ttaatgcggc atgtattttg
    18721 tgttcgctat cggcgtcgac ctcgctggtg gcttcgtcaa aaatgaggat tgaagcgggc
    18781 ttaaggaagg cccgcgcgat ggcgatccgc tgccgctgtc caccggaaag cgcgctgcca
    18841 aactccccca gcggtgtttc gtaaccgtca ggccactgct cgatgaactc ggcggcgtag
    18901 gcctggcggg cggcggcgcg aatctcgtcc aggctggccc gctcgttgcc gtacgcaatg
    18961 ttctcgcgga ccgagcgggc gaagatgacc gggcgttgag ttaccacggc gatctgctcg
    19021 cgcaagttct tcaaactcag ttcccgcacg tcaataccgt ctatggtggt gcgaccttcc
    19081 tgcggttcga gcaaccgcgg cagcactttc aaaagcgtgg atttcccact cccgttcggc
    19141 ccgacgatgg ccaggcattc gcccggacgg acgtcgagcg tgacaccatc caaggccggc
    19201 ggctcttgtc tcggggtgta gcggaaacgc aggttctcga aacgcaccgc gcgcgccccg
    19261 ttaggcggca ggattttcgc ctgtggcggc gagctctctt cgggctggtc cagaaactcg
    19321 aaaatccgcg aagatgcggc gccggcacgc tgcaccacgt tgtacacgtt ggccaccttg
    19381 cgcaccgggt ccagcatggc cgccagaagc gccaccatcg tcacaaagtg atcagcttct
    19441 atgtcgccat ggaactggcg ccaggcgagc cagaccacac ccgccgccgt gcatatcact
    19501 ccaaccgttt cgattgccgg cgagctgatc gcctcaatcc aggccaggcg gaggctctgc
    19561 ttcagcattt ggcgttcgag ccgccacata cgcttgcgct cgtggttctc gcgcgcgtag
    19621 cctttgacta cgtcgatccc ctgcagcgat tcctcaagcg aacctagcat cgcaccgtag
    19681 ccctgcagaa gccgcaccac ggccttgcga atcttgcgac cgtaatacca caggatgagc
    19741 acggccggag gagctacggc caatacgagc agcgtcagcc gcaggtctat gtatagtgcg
    19801 acggccagca cgcaaatggc cttgagcggc tcgcgcgcca ccttgccgaa aagcgtggtc
    19861 acaccggcaa acatctcgcg cgcgtcggac atgaactggc tgacgcggtt ggaaacatcc
    19921 cccgagagct ggcttaccgg cacgcgcagg gccttgcggt acatgagacg gcgcaggtcc
    19981 atcatcatgc gattggaagc atccagaacg aagtacgtcg atgcgcaacg aaggacattc
    20041 ccgaccagat ttatcagcac gagagctgag atcaggatca ggagggtcgt catttgtgct
    20101 tcaggagtca gcgagtcggg gaaaaactgc gccgcccacg tcaagaccgg tgcataccag
    20161 tgtagtgccg tggtcccgtc ggccaacacg acctcttggc tggctgcctt gagcaaggtc
    20221 tgatggaagg tcgtctggtc gttaataatg actctgagga ccggcagcag cccgccgagg
    20281 ctggccgcgt acgtcatcgc gacgcccagc ccgagaacga tccccaacgt catcctccgc
    20341 cggtgcggcc gcacgagggc cagcatgcgc cagaaagcct tgcgcgaccg ggccttagga
    20401 atcgctggct ggcgctgggc cagtacgctg gggaccggcg cggctggggt taggaccgtg
    20461 tcgctcactc ggtgaacctc gtctgggtca aggttgaagg ctctccgtcc ccgatcgcat
    20521 ggcaaggagc gtaaagggac gcttctcttt ccggatcgcc cgccaccata gccggtcagt
    20581 caagacgagg caaccggcag gaccgtaacc agcctgctag cgagcctgtc agaatggcag
    20641 gttccgccgc caaccgccga aacggcgggc tgcccggcgc cgcccgcgac agttggcgag
    20701 aaacgctaag ctccgggttt cacgagtgtt aggcgcagtc ccccgctgcg cggcaggctg
    20761 gcattgtgtt tgcatcaggg cagcagtccg gggccgctcg gcgcggcgga cggcctaatc
    20821 atggtctggc cccgatctgt tttcaggccc ggtgacagac ctccggctca agacgagccg
    20881 gttatgcaaa atactcgcgg ctcgtacgag ccgcctctgc aaaggagacg tctgaatggc
    20941 ggctaagcaa atggcgttcg accaggaagc acagaaggcg atcctggcgg gagtggaaaa
    21001 actggcggcg gccgtgcgca ccaccttcgg gccgcgcggc cgcaatgctg tcctggataa
    21061 gggctggggc gcccccacgg ttacgaaaga cggcgtgacc gtggctgaag aagtggaact
    21121 ccacgacaaa tacgaaaaca tgggggccaa gctcgtgaaa gaggccgcca gcaagaccag
    21181 cgacgtggcc ggcgacggaa cgaccaccgc gacggtcttg gccgaggcga tctacaaaga
    21241 agggctgcgg aacgtcgtgg ccggcttcga tccgaacggc atcaatcgcg gaattgacgc
    21301 tgccgtgcgg gcaatggtgg ccgagctcaa gaacatttcc cgcccggtta atctcgatcc
    21361 caagcgagcc gacgaaatcg tcaacatcgc gtcgatctcc gccaacaacg accgcgcgat
    21421 cggccaaatc atggccgact gcttcgccaa ggtcggcaag gacggtgtca ttacggtcga
    21481 ggagggcaag agcctggaaa cgacggtgga cgtggtcgag ggcatgcagt tcgaccgcgg
    21541 ctacctcagc ccgcatttcg tgaccgatcc ggaagccatg gaatgcgtgc ttgaaaaggc
    21601 cttcgtgctg atctacgagg aaaaaatcag cagcgtgacc aagctggtgc cgctgctcga
    21661 gaaggttgcc caagccaagc gcccgctgct cattatcgcc gaggacatcg agggtgaggc
    21721 gctggcgacg ctggtggtca acaagttgcg cggcattctc caggtatgtg cggtaaaggc
    21781 gcccggctat ggcgaccgcc gcaaagccat gatgcaggac attgcaatcc tgaccggcgg
    21841 caaaccaatc ttcaaggacc tggggatcga gctggattcc gtcaacatcg aggacttcgg
    21901 ccaggccaag cgcatccggg ttgacgccga caacaccacc atcatcgaag gcgccggcag
    21961 cagtgcggac atcaaaggcc gcatcgagca gatccgcaag gagatcgaag acactaccag
    22021 cgactacgac cgcgagaaac tccaggagcg tctggccaag ctggccggcg gcgtcgcgca
    22081 gatcaaggtc ggcgcggcga ccgagtcgga gctcaaggag aagaaggccc gcgtcgaaga
    22141 cgcactgcac gcgacgcgcg ccgcgatcga cgagggcatt gttcccggtg gcggagtagc
    22201 cctgctgcgg gccggtaagg cgcttgaaac gctcaaggta aaaggcgacg acgagaaggt
    22261 cggcgtccag atcgtcaagc gcgccatcac cgcgcccatc aagcagatcg cggccaacgc
    22321 gggctacgag ccggccgtcg tgctcaagcg cgtcagtgag aaagacggcc cctacggctt
    22381 caacgccgaa accggcgagt acggcgacat gatgcaaatg ggcatcatcg acccggccaa
    22441 agtcgtgcgt tcggcactgg agaacggcgc cagcgtggcc cgtgtgctgc tctccaccgc
    22501 ctgtctgatc gccgaaaagc ccaaggagaa gaaggccggc ccgggcggcc cgccgggcat
    22561 gggcggcatg ggcgacgacg acatgggtgg gatggacatg atgtaatgac aacggcgaca
    22621 cgcaccggct gcgcgccgtg ttgcgtggag cagacgaagc catagtccca gaaagggaat
    22681 tggataaacg gtagacaagc aggctcaccg gcgtgccgca gagcccagtt caaggagatt
    22741 gaaagtgcca accgcaactg cgaagaaact gaaaattcgt ccgctggatg atcgggtagt
    22801 cgtcgaacct tccgagcccg aagagaaaac cgccggcggg atcgtgctgc cggacacggc
    22861 ccgcgaaaag ccgctgcacg gcaaggtcat cgccgccggc cccggcaagc tgctcgagaa
    22921 gagcggcgag cgcggcaaga tgtcggtaaa agtcggcgac accgtgatct acggcaagta
    22981 ctccgggacc gaggtcgagc tcgagggcga gaagtacgtg atcctgcggg aaagcgacat
    23041 tctggctata caagagtagg tggcggggcg gaactgtgtg agagccacgt atggcagagg
    23101 ctttgcgcga gttccgggca gaaacgtatg ccgtggcggc gcaggagcac gttacggccg
    23161 ctgtcgagct gcatgaggcg gagcgcttcg cgctcgccca ttacattgcc ggccttgcgg
    23221 tggagtgtgt actccgcgcg tttaagagcc gtctcgatcc tgttctcgat gaacggcatg
    23281 acctgtacca acttgccaaa ggcggccgct tcttcgaccg tgtctcggag aagcacctgc
    23341 ctgatttatt ggcggcattt ggcgaggtcg tagcgcggtg ggacaatggc caccgttacc
    23401 ggtcggaatc tgccctgcgg aagttcctcg tgcaaaggcg gctcttcagc ggcaagggag
    23461 acttgctccg gaacagcagc cggcgaatcg taaatgcggc cttgcaggtc gtcagcttcg
    23521 gagcacaaac atggaagaat tgatcaagcg cctgcgccaa ttgctcgaaa gggtctttcc
    23581 gggcgccacg gtcgagctcg aagtggcagc ccccgagaag gtcggcggct tcctcatctg
    23641 gaacggtttc gaccaagtgg accaggtcga gcggcagcgc agactctgga ctgccattcg
    23701 tggtgaatta tcacgagaag a
//
LOCUS       DAOSYS010000024        23012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1841_length_23012_cov_5.443481, whole genome shotgun sequence.
ACCESSION   DAOSYS010000024 DAOSYS010000000
VERSION     DAOSYS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_23012_cov_5.443481"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLU99_02645"
     CDS             complement(<1..468)
                     /locus_tag="PLU99_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOO15984.1"
                     /translation="MARGVIFDMDGVLVDSGPPHKESWRAVARQYGIDLTDEAFERTF
                     GMSSREIVRKIWGPQITDEEVRQIDDEKERIYRDLIRNNVPLSLGARETVARLAQAPY
                     VLAVGTSGPPENLELVLRASGLGPYFAATVNGLEVSHGKPAPDIFRLAAARLGV"
     gene            complement(559..1911)
                     /locus_tag="PLU99_02650"
     CDS             complement(559..1911)
                     /locus_tag="PLU99_02650"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO15985.1"
                     /translation="MTSRTSPRILSLAALVSVTCLLVGCPPNPTPPVPRPVANAGPDQ
                     TVSVGDEVTLDGSASAPSRAGTLTFTWQQLAGTPVVLDNPHEPIATFLAPDSPTILTF
                     QLIVTESTGRSAEDTTQVTVEGAPPPIPEAILYVANAAGGSVTAYGFDVPFQLDGDLP
                     PAAELIGPINTAVNIPTAVVIENTGTLLVYNAGTPSITGYPDALTLVTIDGDRPPTRV
                     IQGAATNLVGPAAMAYDAANDVLFVADADTDVINVFANASLPFSGNVAPTRVIDSPDV
                     AFPRGLSLGSNGELYVANGLDVDSVAVFDNAATLDGAVFAARLIRSPAFEDLFDAFID
                     VNNTLYVVNGAGGGNQISIFANALTLDGEFLPDAIVTVIGAVNLSAIIVDADGNAYIA
                     DPDNNAIYGYNKLVMRDGLALPDRTLRGPATGLAGPLRMFLVDWTTCATNQLPALSTP
                     "
     gene            2145..>2914
                     /locus_tag="PLU99_02655"
     CDS             2145..>2914
                     /locus_tag="PLU99_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008956722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO15986.1"
                     /translation="MEELDLPSPGARDSGPTKKRLPGPLSWLGRHELATLVLLILVPA
                     GVWAFVVLADAVTEGETESFDERVLLALRNRAVVSDPLGPAWVEDMGRDVTALGGTVF
                     LTLLTLAVSGYLLLDSKRHAALLVLIAVGGGVLLSATLKRSFDRPRPDLFPHGQYVST
                     ASFPSGHSMVSAATYLTLGALLARVQSRPALKAYVLILAALLMFAVGASRVYLGVHWP
                     TDVLAGWTAGATWALLCWLVARWLQRRGQVEGYATETG"
     assembly_gap    2915..3014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3015..4303)
                     /gene="hslU"
                     /locus_tag="PLU99_02660"
     CDS             complement(<3015..4303)
                     /gene="hslU"
                     /locus_tag="PLU99_02660"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOO15987.1"
                     /translation="MSEMTPRQIVEALDRHIVGQDDAKRAVAVAIRNRWRRLQLAPDL
                     REDVFPKNILMIGPTGVGKTEIARRLAQLIKAPFIKVEATKFTEVGYVGRDVDSIVRD
                     LVERSLLLERERAAEGIRERAARLAEDRILDQLLPLGRSEAADPEQVERRQRTREKIR
                     AQMRSGGLEDRTVEIELEERAVPLHVMSTTGLDQMGPEFEKLMERIMPPRTRRRRAPI
                     SEALEIMTQQEIDRLIDQDALQAAAIQRAEESGVVFLDEIDKVCGSPLSEGGGPEVSR
                     SGVQRDLLPLVEGTTVSTRYGLVRTHHILFIAAGAFTQARPSDLMPELQGRFANRVTL
                     QDLTADDYYRILTEPENALIKQQIALLSTEGVTLNVTDEAIQEMARMAYRANEVLENI
                     GARRLYTIIEKVFGEIAFNASDAVNKQVTVDVDYVHMR"
     gene            4539..5669
                     /locus_tag="PLU99_02665"
     CDS             4539..5669
                     /locus_tag="PLU99_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO15988.1"
                     /translation="MNLEELRALFPITRHYNFQNHAAIAPLSGPAAEAMAGYAHEMAA
                     AAYLEGTYYRAAERVRRAAARLLNADPEEVTFVKNTSEGINYVANGIQWVTGDNVVST
                     SMEFAANVYPWLNLESRGVTLKRVAEEDGRIPFDRLAAAIDRRTRVVAISAVQWSNGF
                     RIDLTRLGELCKERGVLFFVDAIQALGVHPFDVRGMNIDFLAADGHKWLCGPEGAGLF
                     YCRRELIGHLHPVEIGYLAMKQGFDTNEIKIDLRDDAQRFDSGVYNLAGVCALGASIN
                     LLLEFGIDQVQKRVKDLTDRLVEGLHRKNWQVHSPRTPSEWSGIVTFSSDAHDLQALR
                     KHLRNEFRIVVASRLGRLRASPHFYNSTEEIDQLIDALPARP"
     gene            5848..6096
                     /locus_tag="PLU99_02670"
     CDS             5848..6096
                     /locus_tag="PLU99_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15989.1"
                     /translation="MKKTATSIRCVPRCGFDNTEVRSVDLPIDYEAEEQELHEALRYW
                     FLSRGISDAIYAFDVDDNGFFAIINDEVYDKRWGTPIF"
     gene            complement(6277..7371)
                     /locus_tag="PLU99_02675"
     CDS             complement(6277..7371)
                     /locus_tag="PLU99_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO15990.1"
                     /translation="MGRLGLTLPLLSLLMLCGCPPPVPSDGGNDGIPSLDSAGNGSFN
                     SATIVPLDGSGFFEFTGSIQSATDVDMYNIGPINPGDRIFVDVRAADPRRLDLVAAIF
                     SSEEFLQYYSDDRANNDFNPLIDFVLRGDPGLYYLGIQQYYQNNATGSYTVTVQITPN
                     AGVPPPAGQVVFLDWDGGENITIPNVGTFDLPPFDAADLGPYEGQTEQMKDRIEEIVA
                     ERYEGYDLVLLNSDDHPIPSGPHSTVYFGGFSQGAFAISEQVDPYNRDHADDAIIFTR
                     TYDVAFRRHPTFEQMAQAVGNTVAHELGHLLGLVHTADCADLMDTRCTNDSILVPQQF
                     GTAVLDHSVFSLGLQNELELLDWILGFVGL"
     gene            complement(7395..8633)
                     /locus_tag="PLU99_02680"
     CDS             complement(7395..8633)
                     /locus_tag="PLU99_02680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HOO15991.1"
                     /translation="MTRSIRAVLLLTCGLAGCVTELAGPDQGLPGVLAPLVAGGDTQQ
                     AAPGAAPDADDNTDIPIRQSIIVQGSVSGKGTYQMFDLGPGALGEQWEVLPNGRNQAR
                     GPFTLVLLDDQFNLLMRTVVSGQSRLSHVARHATPHVYLGVTPSSTSAGGAFDLEVRW
                     QGGQPVPAAVPQLVYLNFEGASAVRVHGREPLVLPPFDAGSVAAHYAGASAEMAHEIA
                     QTIRLDYARYNVLIFSSVDGPPPEDSEYSTIYFGGSDEALLGLADNVDMYNRAKDQAA
                     IVYVESFSPYSLMGLDTEEMALMIGNVASHELGHLLGLYHTDDPEELMDSTGSAWDLT
                     RDQAFGRAPLEGAVFPTGMQNAPLLLEQTVGLNPKAQPSAKLLSAAKIAQRARMREFT
                     RSEISRRCGLCLNPDDIGAE"
     gene            8954..9367
                     /locus_tag="PLU99_02685"
     CDS             8954..9367
                     /locus_tag="PLU99_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO15992.1"
                     /translation="MDAERIIKLSVNAVRELLDSQNIHIQTTHKQPAGARRSPRWPFP
                     GAVEVWLPDSCYGERHMIATMHNLSLHGLAMRTRLPIPTDTPISLAIHEPSLSCYGQA
                     VVRHCTRGPVGYLIGVEFIFNENEEEDDNSEEDAI"
     gene            complement(9559..10407)
                     /locus_tag="PLU99_02690"
     CDS             complement(9559..10407)
                     /locus_tag="PLU99_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOO15993.1"
                     /translation="MELRGLARHRLPAKQAARVQAAGLLGALFQLRRGELDFLPHPLA
                     AYDGRLAAADPGRVSIHLQAAANVHAPAGAVPRGQRRAGFQGGVAADRRGSQAGPILV
                     QFPWSFKYSQAALDWLARLADSFSEFERFIEVRHRSWCQPAALEQMARLGGLCNIDQP
                     QFSECLGPSDHVLGRNAYVRLHGRNFKAWFAKDVPRWERYNYLYTEEELAEWVQRLER
                     MAERAGNVYAIMNNHYRGQAPANALELRALLEGRQVEVPPDLLAAYPRLARLARPVGG
                     QGLFHF"
     gene            complement(10662..12899)
                     /locus_tag="PLU99_02695"
     CDS             complement(10662..12899)
                     /locus_tag="PLU99_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO15994.1"
                     /translation="MRRLTTGIFAALSLVMVADAALQVPVFSPGPNAEQLARSPDDAL
                     QWVSLVDGFHEGASPVITILESTADRTLMHVVIPGFWVESSSADGLTCQKLTIPEESF
                     TAEVGAPQLPIIHALVAVPFGADPSATVRSASETILTGYDVCPCAPPAFDHPAAQAEP
                     LPDAALDQDLTLYPAQVVSLSPPGVWRDLNVVRVDLAAVRYDQATQSLVIINDALVEI
                     EHPNSAGMLQDARRAISPQFDALYQSQVLNYASLDSLNRRAAGGSASQYLIITHPNFA
                     AAIQPLADWHKRAGMDTEILEITTTNPQVIKNAIVERYNQGGLEYVLLVGDPGYIPLA
                     TWSCASDYWYACITGAPDLYADIALGRLSVTNNTQLERQVAKTLEYMTNPAEGSWLTQ
                     TLLVAHMAGGEFQYRACKEYIRTTLIPGTPFTVSTAYGDTIGVNNALVTSRINAGQGI
                     VNYRGHGMGTLWWQWNIVPEDWTTANVHALNNGGYTPIVFNVACQTHRIQDAAESLGE
                     AWLNKYPGGAVASLGATDDGITYPNQNYDKQLYRAFCQEGNYRLGWISNSAATYILVH
                     DPAYAPWNVKMYLWLGDPALEVWTNTPAALAVTHPATALIGSSEFAVSVSADGVPLND
                     ALVCLWKAGEVYQTQSTTEGVATFSIAPATPGTLAVTVTKHDYLPYTADVPVGTLLAD
                     VNADGLVNAFDIDPFVSALTMGEAEFVSQYATWAYWAADCNQDGLVNAFDIDPFVALL
                     TAGGE"
     gene            13174..>14159
                     /gene="cheB"
                     /locus_tag="PLU99_02700"
     CDS             13174..>14159
                     /gene="cheB"
                     /locus_tag="PLU99_02700"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOO15995.1"
                     /translation="MTATPDTNKSHSGSKVRVLIVDDSPTVRAVLRSLLAREPRLELV
                     GSATNGLDALAKIEALRPDVCTLDIEMPHLDGLDVLARAAGRFPVAFIMISTLTQTGA
                     RITFQALRRGAFDYIPKPESVLTARDEFAASLIEKVLAAGRAKGRVRPELEGGVKIPA
                     PAHNSRGWVVGIGISCGGPQTLSALLPAFPADFAPILVTQHMPVEFTPMFARHLDQIC
                     AMRVKEAEAGDQLTPGRILVAPGDAHLCLKRLGERVYVVLDSGPKVSGHRPSADAMFA
                     SMAAVCAPRCVGLVMTGMGRDGSSGIVKLRQAGCPTIAQDEATSLVFGMPAAA"
     assembly_gap    14160..14259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14260..14750
                     /locus_tag="PLU99_02705"
     CDS             <14260..14750
                     /locus_tag="PLU99_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOO15996.1"
                     /translation="CVALEVAAVDVSYVNPFVESVGSVFSTMLGVNPRRQAVRISSGT
                     NGGGARLTSLIGISGRMHGCVALCFPPATALKLASKMLGTELTEINDEVLDAISELVN
                     IVAGSAKAKFNADPPLQLGLPTVVEGVTYRVKYPSKSIWLEVPFSSDAGDFSMEVTYN
                     PN"
     gene            14760..15128
                     /locus_tag="PLU99_02710"
     CDS             14760..15128
                     /locus_tag="PLU99_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO15997.1"
                     /translation="MKALIVDDSLTIRRIVRKALAIVGINDAVDAEDGCAALEAINKT
                     QDFDLILLDWNMPKMSGIDALRTLRQSGNKTPVIMVTTEAEKSRVIDAIKTGANDYLI
                     KPFSPDQLAAKVKNVVAAPQ"
     gene            complement(15144..15761)
                     /locus_tag="PLU99_02715"
     CDS             complement(15144..15761)
                     /locus_tag="PLU99_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO15998.1"
                     /translation="MRTRAAVAPVLIATIAVVALFALIIGGCAVSGYNRAISLSEQVD
                     QRWAQVEVVLQRRYDLVPNLVETVKGYAGHEREIFTEIAHAREKYFQAQNTGKRDEQV
                     EAANGMERALSRLLLLQERYPELRAQASFQDLMTQLEGTENRIAVERQRYTESVRELN
                     TFVRGFPGKIYAAWAGVTKAEQFEVAGAEVRQAPRVDFGTTQPAP"
     gene            complement(15815..16669)
                     /locus_tag="PLU99_02720"
     CDS             complement(15815..16669)
                     /locus_tag="PLU99_02720"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOO15999.1"
                     /translation="MEQKGRFLNKSRAGRALVVLVAAVFTVQLGRAQEIPAKPPAHVV
                     DLAGVVDDATEARLNRYLLELEQKTGAQMLVLTVKTTGGLPIRDFSIRTAEQWKLGQA
                     DKDNGALIVVAVNDRKWDIDVGYGLEPVLTDLFCGRVAREVFVPAFRRNQYSQGIWEG
                     TLILANKVASNAGVTVTGMPVRKMSSRRPQGASSCACLAFVFFFMLLMGVLGRRRRGY
                     RRWGGYSDGGFLPGMILGTMMGSGRRGWGGSLGGGSWGGGGFGGGYGGFGGGGGGSFG
                     GGGASGSW"
     gene            complement(16756..17538)
                     /locus_tag="PLU99_02725"
     CDS             complement(16756..17538)
                     /locus_tag="PLU99_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16000.1"
                     /translation="MSEAKQAAIDVTGIPLNRREDFADFARELVALAGENMLGLCAYG
                     GWLVGDPCFARQPALSVLVLARADLETLDRIAQRGGRYGRHNISAPLSMTPEYIARSC
                     DAFPLEFLEIQQVHHLVYGRDYFADLNLPAHDLRVQCEREVKSELIQLRHGLLAAHGR
                     HKLLHEVCVAAAVRTARVARGLLHLMGKDVPARACDILAATSQAAGVPLATLSTALGG
                     EERLEFSGFQQCYAEVEAFAAFLDAFGSHPAPAQPAPREQAQ"
     gene            complement(17706..20564)
                     /locus_tag="PLU99_02730"
     CDS             complement(17706..20564)
                     /locus_tag="PLU99_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO16001.1"
                     /translation="MRWSVLLFAVGTVAAVVCAQELSGWTQLSLSTCRVDEFLAANPE
                     SDGRGVVIAVLDTGVDPSIPGLTRTPDNAVKVIDIQDFTGDGDVELHRIRLDEQTGKL
                     VEFNEEGEPIEYEPPSVPAAPAGVERLWWLGMFDEAKFINSGQPDLNDNDTKDDKFPV
                     LVTALSNACCNEHAVAFIDTDLDRSFADEKPLQDYKLNYDTFTFHRAKPEKQMVPFTF
                     SLNIFLDKERVVICFDNGAHGTHVAGIAAGYQINNQPGFNGVAPGAKIISLKIGNGSL
                     GGVSTTESMKKAFEYAARYAREHNVPVVCNMSYGVESEIEGFSDIDKVVDKWLRANPY
                     VLFCTSAGNSGPGISTVGTPAAASDCIAVGALMAADTGRDVAGYNMSGPAITVFSSRG
                     GELDKPDVVAPGWSTSTVPRYNQTGDFWAGTSMASPYAAGLCATLISHELQRDPQARV
                     RAADVRRALWLSATPLPGATPLDQGFGLPDLPKAAEILARLVRQAPDDPLIGYDISTS
                     SPFGYQGKSRAAYWRGTWFPPEQDRQVFTIKPVFSPLVDAEERTAFSRKFELRSKAKW
                     CRVPQETFYLRGEQAARVYVEYDAEELKQPGLNVAEVEAVSDGLVAFRLLNTVVVPYR
                     FSADENYIKTFENQSVKGWNPQRYFLEVPPGASAMQLRLSAPEGKVSRASFDRVYDPR
                     GREFRMRGRQLDTEAGTREAEWSFTDELTPGVWEVDVHADRPDREWPYNLGVRFFGLH
                     ANPPRITEADKSKPKGQITVTNLFARPLATTADGQIEGWRLHKDAEFEGRRDELTYDL
                     TLDERFNRVRLRLEMTPENYARTTDIGVMVKNREGEALYQGAFDSRILEATVSTKGNK
                     SLQVVITAGFAVVEEKRKTAITVDIDQLLENPVPVTVKLGEDSNITFVPAVPMTLEYA
                     AKERLKDAPKGLRPVGFLRFRERSSNDEALRVEIDLAG"
     gene            20895..21551
                     /locus_tag="PLU99_02735"
     CDS             20895..21551
                     /locus_tag="PLU99_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO16002.1"
                     /translation="MLIGAIVGPVYAGLIVLYGFSPQATDVGYAPLQPIAYSHALHAG
                     DLGIDCRYCHVTVEDGATAAIPPTQTCINCHSSAYGIRRDSPKLQALLAAYYGTGDRP
                     ETAGLPIPWVRVHDLPDYVYFDHSAHVRHGVGCAECHGRVDRMETVYQVQPLSMSWCL
                     ECHRNPGPRLRPASEITNMTWAGPGGGRGELPDSAARLLKEYNIRGVAELQSCSLCHR
                     "
     gene            21553..>23012
                     /locus_tag="PLU99_02740"
     CDS             21553..>23012
                     /locus_tag="PLU99_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOO16003.1"
                     /translation="MMASKDHNYWRSLDELANTPAFRRWVEDEFPDLWERIATGGNPH
                     TRRQFLKLAGATLALGGLTACRWPKEAIVPATRQPGNRVPGVPVQYATTYELSGVATG
                     VLVTSYDGRPIKIEGNDKHPFSLGKTNHWMQAAVLDLYDPDRSQHILQRTSGTDRYLS
                     RSLDDFRTFAAQHFERLRAVGGAGLCVLSEPSSSPSRLDMKSRFLAAFPQAQWIEYEP
                     ISRDNEHNGARLAFGRPYRSHLHLERADVLVALDSDFLMLHPAALKYARDFAARRRPD
                     AANGYDCNRLYVLESNFSVTGSMADRRYAVRSSDIRTALEQIARAVAARLDVATKLPA
                     PVGDLPLSEATAEAIAADLVAHRGRCAIIVGPQQPAEVHVEAHLLNDWLGTHGETITF
                     TAEPAAERTDYAAGMAALAEQIRAGGVDTLVILGGNPVYDAPAELGFGRASGQSPDAA
                     CAFDKIGTTVRLGLYFDETSRCCDWHLPQAHWLEAWG"
BASE COUNT         4220 a         7138 c         7156 g         4298 t
ORIGIN      
        1 cacgcccagc cgcgccgcgg ccagccggaa gatgtcgggc gcgggcttgc cgtgcgacac
       61 ctccaacccg tttacggtgg ccgcgaagta cggccccagg cccgaggcgc gcaacaccag
      121 ctccagattc tccggcgggc ctgaggtgcc cactgccaac acgtaaggcg cctgggccag
      181 ccgtgcgacc gtttcgcgtg ctccgagcga aagcggaacg ttgttgcgga tcaggtcgcg
      241 gtaaatccgc tctttctcat cgtcgatctg ccgcacctcc tcgtcagtga tctgcggccc
      301 ccaaatcttg cgcacgatct cgcggctcga catgccgaac gtccgctcaa atgcctcgtc
      361 cgtcaggtca atcccgtact gccgcgccac ggcccgccag ctttccttgt gcggcggtcc
      421 cgaatcgacc agcacaccat ccatgtcgaa gattacgcct cgcgccatct ggtatctccc
      481 acgcagacag ggattctaat cgcgcaggga ttaaaggaac cagggaccga gaggccgagg
      541 gattaaggga tccagggatc aaggggtcga gagagcggga agctggttcg ttgcgcaggt
      601 cgtccaatcc acgaggaaca tgcgcagcgg gcccgccaag ccggtggccg ggccccggag
      661 cgtgcggtcg gggagtgcga ggccgtcgcg catcactagc ttgttatagc catagatggc
      721 gttgttgtcg ggatcggcga tgtacgcgtt tccatcggcg tcgacgatga tggcggacag
      781 gtttacggcg ccgatcacgg taacgatggc gtcgggtagg aactcgccat caagcgtcag
      841 ggcgttggcg aagatgctga tttggttgcc gccgccggcg ccgttgacca cgtagagcgt
      901 gttattcaca tcgataaacg cgtcaaatag gtcttcgaat gcggggctgc ggatcaggcg
      961 cgcagcgaac acggcaccgt cgagtgtggc ggcattgtcg aacacggcca cgctgtcgac
     1021 gtccaggccg ttggcgacgt aaagctcacc gttcgagcca aggctgaggc cgcgcggaaa
     1081 agccacgtct ggactgtcga tgacgcgcgt cggggcgacg ttcccgctga agggcagcga
     1141 ggcgtttgca aagacgttta tgacatccgt gtcggcgtcc gcgacgaaga gcacgtcgtt
     1201 ggccgcgtcg taggccatcg cggccggtcc aacaaggttt gttgcagcgc cctggatgac
     1261 gcgggttggg ggccggtcgc cgtcgatggt gactagagtc agcgcgtcgg gatatccggt
     1321 gatcgatggg gtcccggcat tgtagactag cagggttccg gtgttttcga tcacgacggc
     1381 ggtgggaatg ttcactgctg tattgatggg gccgattaat tcggcagccg gagggagatc
     1441 gccgtcgagt tggaacggga cgtcgaaacc gtaagcggtc acgctgccgc ccgcggcatt
     1501 agcgacatac agtatcgcct ccggtatggg gggcggggcg ccttcgacgg tcacctgcgt
     1561 cgtatcttcg gccgagcgtc cggtcgactc agtcacgatc aactgaaacg tcaaaattgt
     1621 tggtgaatcg ggagccagaa aggtggcaat gggttcgtgc gggttgtcca gcacgaccgg
     1681 cgttcccgcg agttgctgcc aggtgaacgt aagcgtgccg gcccgggaag gtgcgctggc
     1741 gctgccatcg agtgtgacct cgtcgccaac cgacaccgtt tggtccggac cggcgtttgc
     1801 gacggggcgc ggaacggggg gcgtcggatt cggcgggcaa cccacgagca ggcaggtcac
     1861 gctaaccagc gcggctaacg acaagatgcg gggggacgtt cgtgaggtca tgccagaccc
     1921 tccttgccgg gtgcatgcgc ttggctatgc gactaccgcg atagacatca gtaatgccca
     1981 gcaccggatg ctaggccggc ggttgacgag atcaatgtta tgcggtcagg caacagctat
     2041 cagcatgtcc gatacttccg cccttgttgc cgcgttggct ggcggcttct tatgatgcgc
     2101 gattagggac aacgtgcgct ttgccgcgcg acgatgctta cacaatggag gaacttgatt
     2161 tgccatcgcc cggcgcccgc gattcaggcc ccacaaagaa gcgactaccc ggcccgttat
     2221 cgtggctcgg gcggcacgaa ctcgcgacac tggtactcct gattttggtg ccggccggcg
     2281 tttgggcgtt tgtggtgttg gccgatgccg tcaccgaggg cgagacagag tcgttcgatg
     2341 aacgcgtgct gctggcgctg cgcaatcggg ccgtcgtctc tgatccgctg gggccggcgt
     2401 gggtggagga tatggggcgc gacgtcacgg ccttgggcgg caccgttttt ctcacgttat
     2461 tgacgctggc cgtgtcgggc tacctgctgc tggacagcaa gcggcacgcg gcgctgctcg
     2521 tgctgattgc cgtgggaggc ggagtgctgt tgagcgcgac gcttaagcgg agctttgatc
     2581 gcccgcggcc ggatctgttc ccgcacgggc agtatgtttc gacggcgagc tttccgagcg
     2641 ggcattccat ggtatcggcc gcgacctact tgacgctggg ggcgctgctg gcgcgggtgc
     2701 agtcgcggcc ggcgctgaag gcgtacgtgc taattctggc ggcgctgctg atgtttgcag
     2761 ttggcgcaag ccgggtgtac ctcggcgtgc attggccgac tgacgtgctg gcgggctgga
     2821 cggcgggggc gacctgggca ctgctatgct ggttggtggc gcgctggctg cagcgtcgcg
     2881 ggcaggtgga ggggtatgcg acggagacgg ggcannnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnncgcatg tggacgtagt ctacgtccac ggtcacttgc ttgttgaccg
     3061 catcagaggc gttgaaggcg atttcgccga acactttctc gataatcgtg tacagacggc
     3121 gggcgccgat gttttccagc acttcattgg cgcgatacgc catacgggcc atttcttgaa
     3181 tagcctcatc cgtaacgttc agcgtcacgc cttccgtgct cagcagcgcg atttgctgct
     3241 taatgagcgc attctcgggc tcggtcagga tgcggtaata gtcgtccgcg gtcaggtcct
     3301 gcaacgtgac ccgattggcg aagcggcctt gcagctcggg catcaaatca ctcggccgcg
     3361 cctgcgtgaa cgccccggcc gcgatgaaca gaatatggtg cgtgcgcacc aatccgtagc
     3421 gagtgctgac ggttgtgcct tctaccagcg gcagcaaatc acgttgcacg ccgctgcgtg
     3481 acacctccgg cccgccgccc tcgctcagcg ggctgccgca gaccttgtcg atctcgtcca
     3541 ggaagaccac gccgctttcc tcggcccgct ggatagccgc ggcttgcagg gcgtcctgat
     3601 cgatcagccg atcgatctcc tgctgcgtca tgatctcgag cgcttcgctg atcggggcgc
     3661 gccggcgacg cgtgcgcggc ggcatgatcc gctccatcag cttctcgaac tcgggcccca
     3721 tctggtccaa tccggtggtc gacatgacgt ggaggggcac ggcgcgctcc tccagctcaa
     3781 tttcgaccgt tcggtcttcc aggccgccgg acctcatctg ggcgcgtatc ttctcgcgcg
     3841 tccgttgccg ccgttcgacc tgttcggggt cggcggcctc gctgcggccg aggggcagga
     3901 gctgatcgag gatccggtcc tcggccaggc gtgcggcgcg ctcgcggatg ccttcggcgg
     3961 cccgctcgcg ctccagcagg agcgagcgct cgaccaggtc gcgcacgata ctgtcgacgt
     4021 cccgcccgac gtacccgacc tccgtgaact tggtggcctc gaccttgatg aaaggcgcct
     4081 ttatcagctg cgccaggcgc cgcgcgatct cagtcttgcc tacgccggtc ggcccgatca
     4141 tcagaatatt cttgggaaaa acatcctcgc gcaggtccgg cgccaactga agtcgccgcc
     4201 agcggttgcg gattgccacc gcaacggccc gcttggcgtc atcctggccg acaatgtggc
     4261 ggtcaagcgc ttccacaatc tggcgcggcg tcatctcgct catggcaaag caccccaaaa
     4321 cagcgttccg ggtcgaaccg gggcagtata cgcgataata tcttggacga aacgcctctg
     4381 ctatggcgca aacgtctccg tgccgataaa ttaacaggtc tggtttcggc ggcggcgtat
     4441 tccaaccgcc gatatgacct gaattcaaaa actgacgcaa atagcgcctt ttggcaagca
     4501 ctaaaattga acggactaaa ggactaagtg tggcagccat gaacctcgaa gaactcagag
     4561 ctctgttccc gattacgcgg cactacaact ttcagaacca tgccgccatc gcgccgctct
     4621 cgggcccggc cgctgaggct atggccggct acgcccacga gatggccgcg gccgcctacc
     4681 tcgagggaac ctactaccga gcggccgagc gcgtccgtcg agcggcggcc cgcctgctga
     4741 acgccgatcc cgaggaggtc acgttcgtca agaacacctc tgagggcatt aactatgtcg
     4801 cgaacggcat ccagtgggtc accggcgaca acgtcgtttc aaccagcatg gagttcgccg
     4861 cgaacgttta cccgtggctg aacctcgaat cccgcggcgt cacgctgaaa cgtgtcgccg
     4921 aggaagacgg gcgcattccc ttcgatcgcc tcgcggcggc catcgaccgc cgcacgcgcg
     4981 tcgtggcgat ttcggccgtc caatggtcga acggcttccg gatcgacctg acgcgcctcg
     5041 gcgagctgtg caaagagcgt ggcgtgctct tctttgtgga tgcgatccag gccctgggtg
     5101 tccatccgtt cgacgtgcgg ggcatgaata tcgattttct ggcggccgac ggacataagt
     5161 ggctgtgcgg cccggaggga gccgggctgt tctactgccg gcgtgagctg atcgggcatt
     5221 tgcacccggt cgagatcggc tacctggcga tgaagcaggg cttcgatacg aacgaaatca
     5281 aaattgatct gcgggatgac gcgcagcgct tcgacagcgg ggtttacaac ctggccggcg
     5341 tgtgcgctct cggggcttca attaatctgc tgctcgaatt tggcatcgat caggtccaga
     5401 agcgcgtcaa agaccttaca gaccggctcg tagaaggctt gcaccgcaag aattggcagg
     5461 ttcacagccc gcggacacct agcgagtgga gcggaatcgt gacgttctcg tccgacgcgc
     5521 acgacctgca agctctgcgc aagcacctgc gcaacgagtt cagaatcgtg gtcgcctctc
     5581 gcctcggccg gctccgggcc agcccgcatt tttataactc caccgaggaa attgaccagc
     5641 taatcgacgc gctgcctgcg cggccgtgag cgtgtaatgt tgtcgttgga ccgaataaat
     5701 tcgtcctctt cgcgcgcggc gggaatacgc cccgccggca cgctgttttt aaattcagcg
     5761 gtttacaaag ctttaaatgg tccgtccgga agagtataat aaagcgtaag ggcagccgga
     5821 ccggattaca ggaaaggctt gtcggcgatg aagaagacgg ccacctcaat tcgatgcgtg
     5881 ccgcgttgcg gcttcgataa caccgaggtt cgatcagtcg atttgccgat cgactacgag
     5941 gctgaggagc aggaattgca cgaggcgctg cgctactggt tcctgtcgcg cggcatctcg
     6001 gacgcgattt acgcttttga cgtagacgat aacggtttct tcgcgattat taacgacgag
     6061 gtatacgaca agcgctgggg aacgccgatc ttctaaggta gacggcgaaa aaaacgcggc
     6121 gttcagcgga cgaccgctta acgagcacga atactgcggc ccgcccgagg caatttgctt
     6181 ccagcggcgc cgcagtttgg gtttagggca gagccgaagc ttaggtcagc ggttcaggct
     6241 aagcgatcag ggaggcgaac tccccgattt tctgaatcac agcccgacaa atcccaatat
     6301 ccaatccagc aactcaagct cattctgtag gccgagcgaa aatacgctgt ggtcgagcac
     6361 ggctgtcccg aattgctgcg gaacaaggat gctgtcgttt gtgcacctcg tgtccatcag
     6421 gtccgcgcag tcggcggtgt gcaccaggcc gagcaagtgg cctaactcgt gggccacggt
     6481 gttgcctacg gcctgcgcca tctgttcgaa cgtgggatgt cggcgaaagg ccacatcgta
     6541 agtcctggtg aagatgatcg catcgtcggc gtgatcccgg ttgtagggat ccacctgctc
     6601 ggaaattgcg aaggcgccct gactgaagcc gccgaagtac acggtcgagt gcgggccgga
     6661 cgggatcggg tgatcatccg aattgagcag caccaggtcg tagccctcgt agcgttcggc
     6721 cacgatctcc tcgatgcggt ccttcatctg ctcggtttgt ccctcatagg gtccgagatc
     6781 cgcggcgtcg aatggcggca gatcaaaggt gccgacgttt gggatggtaa tgttctcccc
     6841 gccgtcccaa tcgagaaaaa caacttgccc ggcgggcggg gggacgccgg cgttaggcgt
     6901 tatttgcacg gtcaccgtgt aactgccggt ggcgttgttc tgatagtact gctgtatacc
     6961 aaggtaataa agacccgggt caccgcgcag cacgaagtct atcagcgggt tgaagtcgtt
     7021 attcgcgcgg tcatcgctgt agtactggag gaactcctcg ctgctgaaaa tggccgccac
     7081 cagatcgagc cggcgagggt ccgccgcacg tacgtcgacg aatatgcgat cccccgggtt
     7141 gatcggcccg atattgtaca tgtccacatc ggtcgccgac tgaatgctgc ccgtaaactc
     7201 gaaaaacccc gagccgtcca gcggcacgat ggtcgccgag ttgaagctgc cgttgccggc
     7261 gctatcaaga ctcggaatgc cgtcgttgcc gccgtccgat ggaaccggcg gcggacatcc
     7321 gcacaacatc agcagcgaca gcagcggaag cgtcagcccc aacctgccca tgcgtgcgca
     7381 acggtcactc tggattattc ggccccgata tcgtctggat tgaggcatag tccacatcgg
     7441 cggctaatct cgcttcgggt gaattcgcgc atccgcgccc gctgtgctat cttagccgca
     7501 ctgagcagtt ttgccgaagg ctgcgccttg ggattgaggc cgaccgtctg ctccagcaac
     7561 aacggggcgt tttgcatgcc ggtcggaaac acggcgccct ccaggggcgc ccgcccaaac
     7621 gcctgatcgc gcgtcaaatc ccaggccgaa ccggtcgagt ccatgagttc ctctggatcg
     7681 tccgtgtgat aaagcccaag caaatgcccc agctcatggc tcgccacgtt gcctatcatg
     7741 agcgccattt cctccgtgtc cagtcccatg agcgaatagg gcgaaaagct ttctacgtag
     7801 acgatggcgg cttggtcctt ggcgcggttg tacatgtcca cgttgtccgc caatcccagc
     7861 aacgcctcgt ccgacccgcc aaaatagatg gtgctgtact cagaatcctc gggcggcggg
     7921 ccatccaccg aactgaagat cagcacgtta tagcgcgcgt agtccaggcg gatcgtttgc
     7981 gcgatctcgt gcgccatctc cgcactcgca cccgcgtagt gcgcggctac gctacccgcg
     8041 tcgaagggcg gcaggacgag cggctcgcgg ccgtgtacgc gtaccgcgct ggcgccctcg
     8101 aagttcaagt acactagctg gggcacggca gccggcacgg gctgcccgcc ttgccagcgc
     8161 acctccagat caaaggcccc gccggccgag gtggacgacg gcgtgacgcc caggtagacg
     8221 tgcggcgtgg cgtgacgggc cacgtgcgac aagcgcgatt gccctgagac aaccgttcgc
     8281 atcagcaggt tgaattgatc atccagcagc accagggtga agggcccgcg cgcttggttc
     8341 cggccgttgg gcaacacctc ccactgttca ccgagcgcgc cggggcccag gtcgaacatc
     8401 tgatacgtgc ctttgccgct tacggaaccc tggacaatga tcgattgccg gatcggaata
     8461 tctgtattat cgtcagcgtc aggcgccgcg ccgggcgcgg cttgctgcgt atctccgccc
     8521 gccaccagcg gcgccagcac accgggcaag ccctgatccg gccctgccag ttccgtgacg
     8581 cacccagcca gaccgcaagt cagcagtaag accgcgcgaa tgcttctagt catcaggtgt
     8641 tcctatgccg gcccccggca gttttcaaac tgccactgcg gttaaatcgg cccgtcgtct
     8701 actgctctcc aggtgcgttt gaacgctgaa gtaatgttga gcgcgtggtt tgccgccgcg
     8761 cgttgcgcag cttatcgccc cgcaagaacc ggcattctga gcgaatcgtc cggtaatgcc
     8821 tcgtgcgggc ccgttcccga tttcattgtt gccgcctggt acagcggccc tcaccaagtg
     8881 cgttccgcag tcgataaatg actgtaaccg ggcgttgcac acggcgcgcc ccgcgccgca
     8941 acggagcacg cagatggacg ccgaacgcat tataaagctt tcagttaacg cggttcgcga
     9001 gttgctcgac agtcagaaca tccacatcca gaccacacac aagcaaccgg ccggagcacg
     9061 ccgcagccca cgctggcctt tcccgggcgc agtcgaagtg tggctgcctg actcctgcta
     9121 tggcgagcgg cacatgatcg ccaccatgca caaccttagc ctgcacggcc tggccatgcg
     9181 cacccgcctg cctatcccaa ctgacacccc catctcgctg gccattcacg aaccgtccct
     9241 gagctgctac gggcaggccg tcgtgcgcca ctgcacgcgc ggcccggtag gctacctgat
     9301 aggcgtcgag ttcatcttca acgaaaacga agaggaagat gacaatagcg aggaagatgc
     9361 aatttgacac gacagatgac agcgggtcgt tagactgcga cttcaattga cgatcaagcc
     9421 atcgccgcgt aatgcccaag cgcggcgctg acaacataac tgaaaactga caagtacctg
     9481 aactgacaac tgcgccatcg cggcgtaagg cgcgccattt ccctattgac gtgacaacca
     9541 agaactaaca actaagaact aaaagtgaaa cagcccctgg ccgcccaccg gccgcgccaa
     9601 gcgcgccagg cgcgggtagg ccgctagcaa atcgggcgga acctcgacct gccgcccttc
     9661 cagcaacgcc cgcagctcca gcgcattagc cggggcctgt ccgcggtagt ggttgttcat
     9721 gatcgcatag acgttcccgg cccgctcagc catgcgctcc agccgctgca cccattcggc
     9781 cagctcctct tccgtgtaca gatagttgta ccgttcccag cgcggcacgt ccttggcgaa
     9841 ccaggccttg aagttccgcc cgtgcaaccg cacgtacgcg ttccgcccca gcacgtgatc
     9901 gctgggccca aggcactcgc tgaattgcgg ctggtcaata ttgcacaatc caccgagccg
     9961 cgccatctgt tccagggcgg ccggctggca ccaggaacga tgccgtacct cgatgaaccg
    10021 ctcgaattcg gagaaactgt ccgcgagtcg cgccagccaa tccaacgccg cctgcgagta
    10081 tttgaacgac cacggaaact gcaccagaat cggcccagct tgcgagcctc gccgatcggc
    10141 cgcaacgcct ccttgaaatc ccgcacgtcg ttggccgcgg ggaactgctc ccgctggtgc
    10201 gtgaacgttt gcggcagctt gaaggtgaat cgaaactcgt ccggggtctg cggcggccag
    10261 gcggccgtca tacgcggcga ggggatgcgg tagaaagtcg agttcacctc gacgcagttg
    10321 aaatagcgcg ccaagtaggc cagcggcttg aacccgcgcg gcttgttttg cgggtagacg
    10381 atgccgtgcc agtcctcgta gctccacccg gctggtccga tccagagttt tgcgcgcgcg
    10441 acggccatgg ctcctggatt atcggcgggg cgggtgatgg tttccaccag tgataaagtg
    10501 ataacgtgat agagtgataa aggggttccg ccgagcaacc tttatcactc tatcacttta
    10561 tcaccttatc acgtcgtcgc gggtgtgcac ggccttcggc actgcttgaa gctgcgcttt
    10621 caagcagtgg cacagggggg acccgcgctg ccgaatcttt tttactcgcc gccggcggtc
    10681 agcagcgcta cgaacgggtc gatgtcgaac gcgttgacca gcccgtcctg gttgcagtcc
    10741 gccgcccagt aagcccatgt tgcgtactgg ctgacgaact ccgcttcacc catcgtcagc
    10801 gccgaaacaa acggatcgat gtcgaaggcg ttcaccaacc catcggcatt aacgtccgca
    10861 agaagcgttc cgaccggcac atcggccgtg tagggcaggt agtcgtgctt cgtaaccgtc
    10921 accgccaacg tgccgggagt cgcgggcgcg atgctgaaag ttgccacccc ttccgtggtg
    10981 ctctgcgtct ggtagacctc gcccgccttc cagaggcaga ccaaggcgtc gtttagcggg
    11041 accccgtcgg cgctgacgga gacggcaaat tcggaactgc cgatgagcgc ggttgcaggg
    11101 tgcgtgacgg cgagcgcggc gggcgtattc gtccacactt ccagggcggg atcgcccagc
    11161 cacaggtaca tcttcacatt ccagggcgca taggcggggt catggaccag gatgtaggtc
    11221 gccgcggagt tgctgatcca gcccaggcgg taattgccct cctggcagaa cgcccggtac
    11281 agttgcttgt cgtagttctg gttggggtag gtgatcccat cgtcggtcgc tcccaacgac
    11341 gccaccgccc cacccgggta tttgttcagc cacgcctcgc cgaggctctc ggccgcgtcc
    11401 tgaatcctgt gcgtctggca ggcgacgttg aagacaatcg gcgtataacc gccgttgttc
    11461 agagcgtgaa cattcgcggt cgtccaatct tcggggacga tgttccattg ccaccacaac
    11521 gtacccatgc cgtgcccgcg gtagttcacg atgccctgcc cggcgttgat ccggctggtt
    11581 accagggcgt tgttcacccc gatcgtgtcg ccgtacgcgg tgctcacggt aaaaggagtt
    11641 cccggaatca gcgtggtgcg aatgtattcc ttgcaggcgc ggtactggaa ttcgccgccc
    11701 gccatatgcg cgaccagcag cgtctgcgtc agccacgagc cctcagccgg attcgtcata
    11761 tactcgagcg tcttggctac ctgccgctcc aactgcgtgt tgttggtgac tgacaggcgt
    11821 cccagagcga tatccgcgta caaatcgggc gcaccggtta tgcacgcata ccaatagtcg
    11881 ctcgcacagc tccacgtagc gagcgggatg tatcccggat caccgacgag caggacgtac
    11941 tcgaggccac cctggttgta gcgttccacg atcgcattct tgatgacttg gggattggtg
    12001 gtagtgatct ccaggatttc cgtgtccatg ccggcccgct tgtgccaatc cgccaagggc
    12061 tggatggccg cggcgaagtt ggggtgcgtg ataatgagat actgcgaagc cgagccgcct
    12121 gcggcacgcc tgttcaaaga atcgagcgac gcgtaattga gcacctgaga ctggtacagc
    12181 gcgtcgaact gtggagaaat ggcgcgccgg gcgtcctgca gcatgcctgc agagttggga
    12241 tgctcgatct cgaccagggc gtcgttgatg attaccagcg actgcgttgc ctggtcatac
    12301 cgcacggccg caaggtccac tctgaccacg ttgagatccc gccagactcc cggcgggctg
    12361 agggagacga cctgggccgg gtacaacgtc aggtcttgat cgagagcggc gtccggaagc
    12421 ggttccgctt gcgcggccgg gtggtcgaag gccggcggcg cgcagggaca cacgtcgtat
    12481 ccggtgagaa tcgtctcgct ggccgagcgg accgtcgcgc tggggtctgc accaaacggg
    12541 acggcaacca acgcatgaat gatgggaagc tgcggcgccc ccacttcggc cgtgaacgac
    12601 tcttccggga tagtcagctt ttgacacgtc aagccgtccg cactggagga ttcaacccag
    12661 aacccgggaa tcaccacatg cataagcgtg cggtcggctg tggactccag aatggttatc
    12721 accggtgaag ccccctcgtg aaagccgtcc acaaggctga cccattgcag ggcgtcgtcg
    12781 ggactgcgtg cgagctgttc cgcgttcggt ccgggtgaga ataccgggac ttgtagcgca
    12841 gcgtcagcga ccatgaccag cgacagagcc gcgaaaatgc cagttgttaa gcgcctcatc
    12901 ttcacactcc taggactgaa aggcctgata ataagataca acgtaagcca taagcgggcc
    12961 aatttcgggc aggaattccc attcgggcat catcgtgccg tttatgaata gcaagatgta
    13021 acgcaatgca ctgcaagacg ttcgcagttt acggactgcg gttccaatcg gtgcgtttct
    13081 ggatgccgaa atacacaagc gagacggggc cgagtaatcc cgataagaaa atagggccgc
    13141 gcccgtcttg gacgagaagc ctgcgcaaac gacatgacgg ctacaccaga cacaaacaag
    13201 agccacagcg gatcgaaagt ccgcgtcctc atcgttgacg acagccccac ggtgcgcgcc
    13261 gtcctccgca gcctgctcgc acgcgagccg cgtctcgagc tggtcggctc cgccactaac
    13321 ggcctggacg cacttgccaa gatcgaagcc ttgcgccccg acgtgtgcac gctcgacatc
    13381 gagatgccgc acctggacgg gctggacgtg ctggcgcgtg ccgcgggccg cttcccggtc
    13441 gcgttcataa tgatctccac gctcacgcag accggggcgc ggataacgtt tcaggccctg
    13501 cgacgcggcg ccttcgacta catccccaag ccggaaagcg tgctgacggc acgcgacgaa
    13561 ttcgccgcga gcctgatcga aaaagtcctc gcggccggca gagcgaaggg ccgcgtgcgc
    13621 ccggagcttg aaggcggagt caagatcccc gcgccggctc acaactcgcg gggttgggtc
    13681 gttggcatcg gcatcagttg cggcggtcca caaacactgt cggcgttatt gccggcgttc
    13741 ccggccgatt ttgcacccat cctcgtgacc cagcacatgc cggttgaatt cacgcccatg
    13801 tttgcgcgcc atctcgacca aatctgcgcc atgcgcgtca aagaggccga ggcgggcgac
    13861 cagctcactc caggacggat tctcgtcgcg cccggtgacg cgcacctgtg tctcaaaagg
    13921 ctcggagagc gcgtctatgt agtgctcgat agcgggccaa aagtctccgg acaccgccct
    13981 tcggccgacg cgatgttcgc ttccatggcg gccgtctgcg ccccgcggtg cgtcggcctg
    14041 gtcatgacgg gcatggggcg tgacggcagt tccgggatag tgaaactgcg ccaggccggg
    14101 tgtccgacga ttgcccagga cgaagccacc agcctggttt tcggaatgcc ggccgcggcn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atgtgtggcc ttggaggtcg
    14281 ccgctgtgga cgtttcttac gtcaatccgt tcgtggagag tgtcggttcg gttttcagca
    14341 ccatgctggg cgtaaacccg cggcgccaag cggtgcgaat cagctccgga acaaatggcg
    14401 ggggcgcccg tctgacctcg ctgatcggta tcagcgggcg gatgcatggc tgcgtggcat
    14461 tgtgcttccc gccggccacg gccttgaagc tggccagcaa gatgttggga acggagctaa
    14521 ccgagatcaa cgacgaagtg ctcgacgcca tttcggagtt ggtgaacatt gtggccggct
    14581 cggccaaggc gaagttcaac gccgatccgc cgctgcaact gggtctgccg acagtggtcg
    14641 agggcgttac ataccgggtg aagtacccta gcaagtcaat ttggctggag gtcccgttca
    14701 gctcggacgc gggcgacttc agcatggaag tcacctataa cccgaattga ggccgcccga
    14761 tgaaagcatt gatcgtcgac gactcgctga cgatccggcg gatcgtgaga aaggccttgg
    14821 ccatcgtagg cattaacgat gcggtggacg cggaggacgg ctgcgcggcc ctggaggcga
    14881 tcaacaagac ccaggatttt gacctgatcc tgctcgattg gaacatgccg aaaatgagcg
    14941 ggattgatgc gttgcgaacc ctgcgtcagt ccggcaacaa gacgccggtc atcatggtca
    15001 cgaccgaggc ggagaaatcg cgcgtgatcg acgccattaa gaccggcgcc aatgattacc
    15061 tcatcaaacc cttttcgccc gaccagctcg ccgcgaaagt aaagaacgtg gtcgcggccc
    15121 ctcaatgacg acgccctgac cgctcaaggc gcgggctggg tcgtcccgaa atccacgcgc
    15181 ggcgcctgcc gcacctccgc gccggcgacc tcgaactgct cagcctttgt aacaccggcc
    15241 caggcggcgt aaatcttgcc cgggaacccg cgtacgaacg tgttcaactc acgaaccgac
    15301 tcggtgtagc gctgccgctc gactgcgatg cggttctccg tgccctccag ttgcgtcatt
    15361 aaatcctgaa agctggcctg ggcgcgcagc tcgggataac gctcctgcaa caagagcagg
    15421 cgcgaaaggg cgcgctccat cccgttggcc gcttcgactt gctcgtcgcg cttgcccgtg
    15481 ttctgggcct ggaagtactt ctcgcgtgcg tgcgcgattt ccgtaaatat ttcgcgctcg
    15541 tggccggcgt accccttgac cgtttcgacc aggttcggaa ccaggtcata gcggcgctgc
    15601 agcactactt ccacctgggc ccagcgttga tccacctgtt cagatagtga tatggcacgg
    15661 ttgtagccgc ttacggcgca cccgccgatg atcagggcga acaacgccac cacggcgatc
    15721 gtggcaatca gaaccggcgc aactgccgct cgtgttctca tgtccgtaaa cctcggcatc
    15781 tgatctagtc tatgctctga cattcctact acgctcacca acttcccgac gctccaccgc
    15841 cgccgaacga accgccgccc ccgccgccaa agccgccgta cccgccgccg aagccgccac
    15901 cgccccaact gccgccgccc agcgagccgc cccagccgcg acgcccgctg cccatcatcg
    15961 tgcccagtat catccccggc aggaaaccgc catcgctgta tcctccccag cggcgatacc
    16021 cgcgccgccg ccgccctagc acccccatca acagcatgaa gaaaaatacg aacgccaaac
    16081 aggcacaact gctggcgccc tgcggccgcc gtgagctcat cttgcgcacc ggcattccgg
    16141 tcacggtcac gcccgcgttc gaggccacct tgttggccag gatcagcgtg ccctcccaaa
    16201 taccctgcga atattggttc ctgcggaaag ctgggacgaa gacttcccgg gctacacgcc
    16261 cgcagaacag gtcagtcaac accggttcca gcccgtagcc gacgtcgatg tcccacttgc
    16321 gatcgttcac ggccaccacg atcagcgcgc cgttgtcctt gtcggcttgc ccgagcttcc
    16381 attgctcggc tgtgcgaatg gagaagtcgc ggatcggcag gccgccggtc gtctttacgg
    16441 tcaacaccag catttgcgcg ccggtctttt gttccaattc cagcaggtag cggttcaggc
    16501 gtgcttccgt cgcgtcgtcc acgacacccg ccaagtccac cacgtgggcc ggcggcttgg
    16561 ccgggatttc ctgtgcgcgg ccgagctgga cggtgaagac ggcggccacg agcacaacga
    16621 gagcccggcc cgccctgctc ttgtttagga atcgtccttt ctgctccacg tcagttcctg
    16681 atgctaaaac attcgcggcc ctgcggcgat tattaatccg ctgctggttt ggaacgggcc
    16741 ggcggccagg gctgctcatt gcgcctgttc ccgcggggcg ggctgggccg gcgcggggtg
    16801 cgacccgaag gcgtccagga atgccgcgaa agcctcgacc tccgcatagc attgctgaaa
    16861 gccgctgaat tcgaggcgct cttcgccgcc cagagcggtg ctcaaggtgg ccaaaggcac
    16921 gcctgcagcc tgggaggtcg cggcgagaat gtcgcaggcg cgcgcgggga cgtccttgcc
    16981 catcaggtgc agcagacccc gcgcgacgcg cgccgtgcgc acagcggccg ccacacacac
    17041 ctcgtgtagc agcttatgcc ggccatgagc ggccagcagc ccgtgccgca gttggatcag
    17101 ctcgctcttg acttctcgct cgcactgtac gcgtaagtca tgcgccggca ggttcaagtc
    17161 ggcgaagtaa tcgcgcccgt agaccaggtg gtgcacctgt tggatctcga ggaactcgag
    17221 gggaaaggca tcacaggagc gcgcgatgta ctccggcgtc atgctgagcg gcgcgctgat
    17281 gttgtggcgg ccgtaacgtc cgccacgctg ggcaatgcga tcgagcgtct ccaggtcggc
    17341 tcgggccaac acgagcacgc tcagggcggg ctggcgcgcg aaacacggat cgcccacgag
    17401 ccacccgcca taggcgcaca ggcccagcat gttctcgccg gccaaagcga cgagttcacg
    17461 cgcgaaatca gcaaaatcct cccggcggtt gagcgggatg ccggttacgt caatggccgc
    17521 ctgttttgct tcgctcatgg cgcctcgcgt ctggtggagt tttgcagcga tttactatag
    17581 ccgcttgcgc tgcttgaagt taagcagcag aagcgatcag gtaagcagcg gaagcgatcg
    17641 agcatgatcg cttccggctt acctggttcg acgtaatgcg gattcagcga ctcgcgtcgc
    17701 gcgcttcaac ccgccagatc aatctccacg cgcagcgctt cgtcgttgct ggagcgctcg
    17761 cggaagcgca ggaatccgac cggtcgcagg cccttgggcg cgtctttcag ccgctctttt
    17821 gccgcgtatt caagcgtcat aggaacggcc ggcacgaacg tgatgttcga gtcttcgccc
    17881 agcttcacag tgaccggcac cgggttttcc agcagttggt cgatatccac ggtaatcgcg
    17941 gtcttgcgct tctcctccac taccgcgaaa ccggcggtaa tcactacctg gagcgactta
    18001 ttccccttcg tgctgaccgt tgcctcgagt atgcgcgagt caaaagcgcc ctggtagagc
    18061 gcctcgccct cgcggttttt gaccatgacg ccgatatccg tagtgcgtgc gtagttctcg
    18121 ggcgtcatct ccaaacgcag gcgaacgcgg ttgaagcgct cgtccagcgt cagatcgtaa
    18181 gtcagctcgt cccgtcgccc ttcaaattca gcgtccttgt gcagacgcca accctcgatc
    18241 tggccgtcgg ccgtggtcgc cagcggccgg gcgaacaggt tggtcaccgt gatctggccc
    18301 ttcggcttgc tcttgtcggc ctcggttatg cgtggcggat tcgcgtgcag cccgaagaag
    18361 cgcacgccca ggttataggg ccattcgcgg tccgggcgat cggcgtgcac gtccacctcc
    18421 cacacgcccg gcgtcagttc gtccgtaaag gaccattcgg cctcgcgcgt cccggcctcc
    18481 gtatccagtt gcctgccgcg catgcggaac tcgcggccgc gcggatcgta cacgcggtca
    18541 aagctggccc tgctgacttt gccctccggc gcgctcaggc gcagttgcat cgccgacgcg
    18601 cccggcggaa cctccaggaa atagcgttgc gggttccagc ctttaaccga ctgattttcg
    18661 aaagttttga tgtagttctc gtcggcgctg aagcggtaag gcacaacgac cgtgttgagc
    18721 agccggaatg ccaccaggcc gtcgctaacg gcctcgacct cggcgacgtt caggccgggc
    18781 tgcttcaatt cctcggcatc gtattccacg tacacgcgcg ccgcctgttc gccgcgcagg
    18841 tagaaagtct cctgcgggac gcggcaccac ttggccttgg agcgcagttc gaatttgcgc
    18901 gagaaggccg tgcgctcttc cgcgtccacc agcggcgaaa acaccggctt gatcgtgaag
    18961 acctgtcgat cctgctcggg cgggaaccac gtgccgcgcc agtacgccgc acgcgactta
    19021 ccctggtatc caaaaggcga agacgtggaa atgtcgtagc cgatcagcgg atcgtccggc
    19081 gcctgcctta ccaggcgcgc caggatttcg gccgctttgg gcaaatcggg cagaccgaag
    19141 ccctggtcga gcggcgtcgc gccgggcagg ggcgtggccg aaagccacaa ggcccggcga
    19201 acgtccgcgg cccgtacgcg cgcctgcgga tcgcgttgca gttcatgact gatcaaggtt
    19261 gcgcacagcc cggccgcgta cggactggcc atgctggtgc ccgcccagaa atcgccggtc
    19321 tggttgtagc gtggaaccgt ggaggtcgac caacccggcg cgaccacgtc cggcttgtcc
    19381 aactcgccgc cgcgcgaact gaagaccgtg atggccggtc cggacatgtt gtagccggca
    19441 acgtcgcgtc ccgtatcggc cgccatcagg gcaccgacag cgatgcagtc gctggcggcg
    19501 gccggtgtgc cgacggtgga aatacccggt ccggaattac ccgcggacgt acaaaacaga
    19561 acataaggat tcgcgcggag ccacttgtcc acgactttgt cgatgtccga aaagccctcg
    19621 atttcggatt ccacgccgta ggacatgttg cagacgaccg gcacgttgtg ctcgcgcgcg
    19681 tagcgggccg cgtactcgaa agctttcttc atgctctcgg tcgtgctgac gccacccagg
    19741 ctgccattgc cgatcttcaa gctgatgatc ttggctccgg gcgccacgcc gttaaatccg
    19801 ggctggttgt tgatctgata gccggccgcg atgcccgcca cgtgcgtgcc gtgcgccccg
    19861 ttgtcgaagc aaatcacgac ccgctctttg tcgaggaaaa tgttgagcga gaaggtgaac
    19921 gggaccatct gcttttcggg cttggcccgg tggaacgtga acgtgtcgta gttgagcttg
    19981 tagtcctgca gcggcttctc gtcggcgaag ctgcggtcaa ggtcggtgtc gatgaatgcc
    20041 accgcatgct cgttgcagca ggcgttgctg agggccgtga ccaataccgg gaatttgtca
    20101 tcttttgtgt cgttgtcgtt caaatccggc tggccggagt ttatgaactt cgcctcgtcg
    20161 aacattccga gccaccatag ccgctcgacg ccggcgggcg ccgccggaac gctgggcggc
    20221 tcgtattcga tgggctcgcc ctcttcattg aactcaacca gcttgccggt ttgctcgtcc
    20281 aggcggatgc ggtgcaactc gacgtctccg tcgccggtga aatcctggat atcgatcacc
    20341 ttcaccgcgt tgtcgggcgt gcgtgtcagc cccggaattg acggatccac gccggtatcg
    20401 agaacagcaa tcacgacccc gcgcccgtcg ctctccggat tcgccgccag gaattcatcc
    20461 acgcggcagg ttgataaaga aagctgagtc cagccggaaa gctcctgagc gcaaacgacc
    20521 gcggctacgg tgccgacggc aaacaacaaa acggaccaac gcatgcacgc cacctccaat
    20581 cttggcacga aaactccagg ccggaactgt aacagcccgg cggccgtagc gaaaccaccg
    20641 cccatcagcg gcaggcgccg gtgagcggag gccagcagga cccgggtaag cggagttcga
    20701 aaggagcgcg cggcgaaggc tcggctgagc ttctgtcggc cggcctccct gccttacagc
    20761 cgcgtaatgc gcctcggcga ttccagtgtt gaccatctgt cggcgaggcg ctatataggc
    20821 tatggtcgaa cctgagggag aaagctgtca cacggtttct tttcccaagc tggagcaact
    20881 acatccgcgt cgcggtgtta atcggcgcga tagtggggcc ggtgtatgcc gggctgatcg
    20941 tgctgtacgg tttctcaccg caggctacgg acgtggggta cgcgccgcta cagccgatcg
    21001 catacagcca cgctctgcat gccggcgact tgggaatcga ttgccgctac tgccatgtca
    21061 cggtcgaaga tggcgcgacg gccgccattc cgcccacgca gacgtgtatt aactgccaca
    21121 gcagcgcgta tggcattcgg cgtgacagcc cgaagctgca ggcgttgctc gcggcttatt
    21181 atggcactgg cgaccggccg gagaccgccg ggctgccgat accctgggtg cgcgtccacg
    21241 atctcccgga ttacgtctac tttgaccaca gtgcacacgt gcggcacggc gtcggttgtg
    21301 ccgagtgcca cgggcgcgtg gaccggatgg agacggtgta ccaggtccag ccgctgagca
    21361 tgagctggtg cctggaatgc catcgcaatc ccggaccgcg gctgcgcccg gcgtcagaga
    21421 tcacgaatat gacttgggct ggaccggggg gcggccgggg cgagttaccg gattcggctg
    21481 cgcgactgct gaaggaatac aacatccgcg gcgtcgcaga actgcagagc tgctcactgt
    21541 gtcaccgtta gcatgatggc cagcaaggat cacaactact ggcgcagtct cgatgagctg
    21601 gcgaacacgc cggcctttcg gcgctgggtc gaggacgagt ttcctgacct gtgggagcgc
    21661 atagcgaccg gcggcaatcc ccatacgcgg cgccagtttc tgaaactggc cggcgcgacg
    21721 ctggcgctgg gcgggctgac cgcctgccgg tggccgaagg aggcgatcgt gccggccacg
    21781 cgccaaccgg ggaaccgcgt tcccggcgtg ccagtgcagt acgccacgac ttacgagctg
    21841 agcggggtgg cgacgggtgt gctggtcacg agttacgacg gccggccgat caagatcgag
    21901 gggaacgaca agcatccgtt cagtctgggt aagacgaacc actggatgca ggcggccgtg
    21961 ctggacttgt acgatccgga ccgcagccag cacattctgc aaagaacttc gggcacagac
    22021 aggtatttgt cgcgatcact ggacgacttc aggacgttcg cggcgcagca tttcgagcga
    22081 ctgcgagccg tgggcggggc cggattgtgc gttttgagtg aaccgtcgtc ttcgcccagc
    22141 cgcttggaca tgaagtcgcg cttcctggcg gcgtttccgc aggcacagtg gatcgaatac
    22201 gagcccatat cgcgcgataa tgagcacaat ggggcacgtc tggcgttcgg ccggccgtat
    22261 aggtcgcatt tgcacttgga gcgggccgac gtgctggtcg cactcgacag cgatttcttg
    22321 atgctgcatc ccgccgcgct caagtatgcg cgggacttcg cggcgcggcg ccggccggat
    22381 gcggcgaatg gatacgactg caaccggcta tatgtgctgg agagcaattt cagcgtaacc
    22441 ggcagcatgg ccgaccggcg ttatgctgtg cgttcttcgg atattcgcac ggcattggag
    22501 caaattgccc gggcggtcgc cgcgcggctg gatgttgcga cgaagctccc ggcccccgtc
    22561 ggcgacctgc cgctgtccga ggccaccgcg gaggcgatcg cggcggacct ggtggcgcac
    22621 cgtggccgct gcgccatcat cgttggcccg caacagccgg ccgaggtgca cgttgaggcg
    22681 catttgctga atgattggct cgggacgcat ggcgagacga ttacgtttac ggctgagccg
    22741 gcggccgagc ggacggacta tgcggcgggc atggcggcgt tggcagagca gatcagggcg
    22801 ggtggcgtcg acacgctagt aatcctcggc ggcaatccgg tttatgacgc gccggcggag
    22861 ctgggcttcg ggcgtgcgtc cggccagtcg ccggatgccg cgtgcgcctt cgacaagatt
    22921 gggacgacgg ttcggctggg tctctatttt gatgagactt cgcgctgctg cgattggcat
    22981 ttgccgcagg cccattggct ggaagcttgg gg
//
LOCUS       DAOSYS010000025        22866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1857_length_22866_cov_5.763974, whole genome shotgun sequence.
ACCESSION   DAOSYS010000025 DAOSYS010000000
VERSION     DAOSYS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_22866_cov_5.763974"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="PLU99_02745"
     CDS             <1..1263
                     /locus_tag="PLU99_02745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO16004.1"
                     /translation="FDVVLTDLRMPGMDGVGLLREMRRLGLDVPVILMTAYASVATAV
                     EAMKLGAFDYIQKPFNADEVAILVERAVRDRQLVRDNEVMRRTLEDVESQRKLIGSSP
                     AMRPVLEKLQRVAQSSATVLIMGESGTGKELIARAVHAASPRAAQPMLCVNCAALSPT
                     LLESELFGHEKGAFTGADRMRKGRFELADGGTLLLDEVSEIAPPLQAKLLRVLQEREF
                     ERVGSSVTRRIDVRVIATTNRDLREWAAKARFREDLYYRLSVLPIEVPPLRERREDLP
                     LLLDYFLGRIKVREGRERPTFTPEAMALLAEYSWPGNVRELQNLCERLAVLEAGREIG
                     PDTVRPLLIGPLKTASAPAEGLRYREGHILEDSEQQLILSTLERFNGHREKTARALGI
                     GLRTLGLKLKKWREEGRFPELVVQRKVL"
     gene            1348..1785
                     /locus_tag="PLU99_02750"
     CDS             1348..1785
                     /locus_tag="PLU99_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16005.1"
                     /translation="MWIDGLIDGRTTRAAELTARFAEARHNVLAQNLANIDTPDYHSR
                     NLDPRLFQESLAAALERATQCNSSRLDLRGNAQFSTTPTGQAEVRPAVEPAPNVLFHD
                     GTNNRLEGLIADATENALYYEMSISFLRNSFQNVLSAIRGRVA"
     gene            1802..2212
                     /gene="flgC"
                     /locus_tag="PLU99_02755"
     CDS             1802..2212
                     /gene="flgC"
                     /locus_tag="PLU99_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014696170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOO16006.1"
                     /translation="MIGTLDITRSALIAERTRLDVIAGNIANANVTRQADGTILPYRR
                     RFATFMSGDGRGGPGVHVDSIEQDPSDFKLRYAPGSPDAIPSGPLAGYVQLPNVNITM
                     EYVDALEASRAYEANVAMLNVTKSMLQQAIRLFA"
     gene            2219..2530
                     /gene="fliE"
                     /locus_tag="PLU99_02760"
     CDS             2219..2530
                     /gene="fliE"
                     /locus_tag="PLU99_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOO16007.1"
                     /translation="MIDPVSSRPVSPICPPATSAPATAPGAAGGEASFAALLRRQLEQ
                     VSDMDNQAKQGVQDLLTGRSQNITEVFVAARKAEVAFSLLMEIRNKLSDAYTEIKNLR
                     V"
     gene            2587..4323
                     /locus_tag="PLU99_02765"
     CDS             2587..4323
                     /locus_tag="PLU99_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16008.1"
                     /translation="MESLQKLLARVFTQTRDLSRSQRLAILLGGLLVGVSLLGLLRWA
                     ATPEMTPLLDQELQPDELARVRTGLELIKEPFRLVGQKILVRADANRSAILAQLQQQD
                     KLPSDTSAGFSALVKESNPWISQAEHERRWTVALKQELEQVLRQFRGVKSASVFLPLN
                     QERRRFAQQEAAATASITLVMAGGEPVGRDLALAAARLVCGAVRGLPLRNIEVLDGNG
                     VSALDWDSETAAGSSLDRQRRKAEQDIRAKIIAQLPDPKSRVAVQVELELTNKTSEIQ
                     APTDPIETRVETTAERTVRARPHGQPGVVPNVGVSAGIPAADEGTSKETSSTEYTSGY
                     RRTQEATSPGGMKEVWAAVNLSRSYLEHVFRQSQPSAGEPNEEQLQQVFEREKPRVVS
                     QVKKLVKPQDDAHVAVDWYYDTVLETEPAVVRATAGDEVLDYVRRYGAQSGLGLLAVI
                     SLGLMLRMARKRDGGEAFGLEIGLPKESIEAARRAATDVAKAARREQSRRTGQAAAPG
                     QAAGEALEVMPSAIGQAATTEGVLVAQEVDERTVQTNKMLDQVAQVVDGDAEMAATLL
                     EQWIQRNDALER"
     gene            4333..5370
                     /gene="fliG"
                     /locus_tag="PLU99_02770"
     CDS             4333..5370
                     /gene="fliG"
                     /locus_tag="PLU99_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOO16009.1"
                     /translation="MARFSLKTPDEPSGLTGIRKAAILMLALRQDTAAKILAGLTRDR
                     VEEISREIASIDMVEPELREQVVNEFYNLVLARRYVDQGGLAYARNLLMKTLPSDEAK
                     RIMATIEHSIHQQPFSFLQKTEKENLITFLQEEHPQTIALVLSYLPPVMASEVMTGLP
                     TERQIEVVTRIAQMDQTSPDIIKEVERGLEKRLAGLVSERFERVGGIQAVAEILNLAG
                     RATEKAILEGLEENEPGLVEEIRRLMFVFEDIMRVNDKGIQAVLKEVENEELALALRT
                     ASEELKEKVFRNMSERAAQIIKEEMEFMGPVRVADVEQAQQKIVDIVRRLEDSGEVIV
                     AGKGGDRDLIV"
     gene            5452..6249
                     /locus_tag="PLU99_02775"
     CDS             5452..6249
                     /locus_tag="PLU99_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOO16010.1"
                     /translation="MAGIIRKETVTRTAAFSFADLEQQGRAIIERAQAEAREILAAAQ
                     RHALGLAERERCRALEEGRAEGLAEGRAAGLEQIRAEAHAAAMREAQADVQRLTQSLQ
                     AALAEFDQHKRSLITAAESGLIELALRVARRVCKLEAGRSIGVALANGRALLDMAQHA
                     EDPELCFNPADLEMLKESGAQLAAAVQQFSHVKLVADEKVERGGCLLRTRAGEIDAGL
                     ETQVQRIAEALCVTVADVEPTPPPAPSPELPATNARPPLTTEGNTAT"
     gene            6246..7745
                     /locus_tag="PLU99_02780"
     CDS             6246..7745
                     /locus_tag="PLU99_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOO16011.1"
                     /translation="MTAAAAQNSLAYDRARLSRPLAAAAATDVFAHVADEVSGLLSER
                     IIGRVAAITGMTVYAADLPVPVGARCVIHTRRSGRIYAEAVGLLSNRTVLSVFGEATG
                     IAPDDPVECLSGPPRVPVGFDLLGRVLGSQGEPLDGRPAPRLNIRYPLYCDAPQALQR
                     RPIDQPLGLGIRSIDGLLTAGRGQRLGIFAGTGVGKSVLLGMICKHTQADVNVLALIG
                     ERGREVRDFVERQLGPAGLARSVLVVCTSDGAPALRVRAGFHATAIAEFFRDQGLDVL
                     LMMDSLTRLAMAQRQIGLAAGEPPTAKGYPPSVYALLPRLLERAGRTQSGSITGMYTV
                     LVEGDDIDEPLADVVRGILDGHIWLSRSLANRGHYPAVDVLNSISRVADDVIDAEQRS
                     AALAVRRVLATWHDIEDLVSIGAYVPGANLDYDVAVQTRAAIEDFLRQDRTSGCTFAQ
                     AAAALKQLGQLIQSTTQQLQARRPAPAVDAPRRPAQDGARKNTRTQNGK"
     gene            7880..8365
                     /locus_tag="PLU99_02785"
     CDS             7880..8365
                     /locus_tag="PLU99_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HOO16012.1"
                     /translation="MRRFKFPLQTLLKVRKLREREAKRKVAAQRADIARLDRLNEATW
                     AEISAQHAGLLQNQQQPFIKPADLARTRTWIAHLRATIAQRTLLRAEMTARLQQLQSE
                     YNAARQEARIIEKLRQRRWDEYRRRRARKEQSAADELAQQLQTKAAGDSAGPRPTAAV
                     P"
     gene            8453..9142
                     /locus_tag="PLU99_02790"
     CDS             8453..9142
                     /locus_tag="PLU99_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16013.1"
                     /translation="MLSRLYNLLSTVSIATLLAGGGFSAFLFGTGRLNAARLERIGAV
                     VRGELDEFPQSQPATQPAELDEPPVGRDQSLEQIDAARRQRRLQSHLLERAARDIEAQ
                     RRLLDQSLQYLVNEREQLAAQQADWAAEQQKVTGEVVNEAAQKHLELFAGLAPQQAKE
                     HLVWLWSTNKAEALALVMQLEVATTKRILAQMKAPEEQKILSELLEQLRTYEASGAAT
                     MSRKTAGDAAP"
     gene            complement(9260..9589)
                     /locus_tag="PLU99_02795"
     CDS             complement(9260..9589)
                     /locus_tag="PLU99_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16014.1"
                     /translation="MRLAFGLGLGSGRRTGAAGARVLASGRVGQRALEVVTLQRGLLL
                     LLVGHLTLGGTTARRHFAFGFCRNLGERGTGVGSGCGGRLLGRGIRVALATIILLAFG
                     LMLFAQR"
     gene            9986..10489
                     /locus_tag="PLU99_02800"
     CDS             9986..10489
                     /locus_tag="PLU99_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOO16015.1"
                     /translation="MSNSGPEAASPQTGKNDANFERILRVLRAQISHNRSQTTLQLDP
                     PELGKIRLHLGLRDDTLTLRVEPQTQVAYRLLYEHLDSLRDGLHASGVHLEHVEIRPP
                     AAQAAPQAFDLPPHSGGGGTGGGAGAETDHPQHSGTDSPAAGPSQNAALDEDSEPSAE
                     PRVNIWA"
     gene            10555..11160
                     /locus_tag="PLU99_02805"
     CDS             10555..11160
                     /locus_tag="PLU99_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOO16016.1"
                     /translation="MQTNAINGVTSANSGSSSSAGLNRMTSEDFYNLLVTELQQQDPL
                     EPTKTSDMIGQVSQIRSIEQSAQLTQTLQQLAQQQRTAGAGDLLGKYVEGVGLNVGGE
                     PALLSGVVIAIRFMADGTAVLELDNGAMLSANNLTKVTECADDAGATDAANTANTTNT
                     NAAAAAKRRTPSSENAYSNTIQSWLDSLLHPQPRTATSLQK"
     gene            11188..12870
                     /locus_tag="PLU99_02810"
     CDS             11188..12870
                     /locus_tag="PLU99_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOO16017.1"
                     /translation="MGLTTAMYTGLTGINANQTRINTIGNNIANVNTTAFKGSRTLFQ
                     SQLAHTMSLGTAPSATTGGTNPIQIGLGVDVGATQRNFTPGSLETTGIASDLAIDGRG
                     FFVLRDAGGGQVYTRDGSFALDAQNRLVTTDGKFVQGFGVDSGFNIVPGVLTDITIPL
                     GTASMARPTSTVIMDGDLSAAGTVATRGSEHLSQALVDGAGNAATAGTALTNLFSAAD
                     PGTALFATGNTIRVSGVVKGERELPARSFVVGVDGTTLGDFANWLQGALGINTDAGLP
                     GTPGVTIENGALVIRGNAGEQNSFEISGNDFASDNATVSLPFQFAQTAAADGSGVFTS
                     FTAYDSLGTPVTVNVTFALERTPNGGPVWRYYAESPDARDGSRVLGTGTVAFDNEGNF
                     VGATGNQFTLSRDSTGAATPLTFTLDFSGVNGLSTHTSSIVASEQDGFPPGTLVNFGV
                     GEDGMIYGTFTNGLTQRLGQVAVATFANEEGLVGLDDNCYGVGPASGNATIGAPGQLG
                     AGTVLSGALELSNVDLSREFIGLVTSSTGFQASSRVISVSSDLLNQLLMIIR"
     gene            complement(12937..14955)
                     /locus_tag="PLU99_02815"
     CDS             complement(12937..14955)
                     /locus_tag="PLU99_02815"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16018.1"
                     /translation="MAGHKDEGLNTAPRGRAGVLFAFVLALALLLFLIDTQVPLGLVV
                     LLGYLPLLLVTVAWGGPRYYWLMAVTASGLIVLGHFVSPPAPLLNWIEVHNRALAIAA
                     IWIVSAIGRILWNTRFRLLEQHKHYSRTLADSLAESEARAAELKAIFDSLNQPVLVFN
                     DLGKPVRANRAAHELLAADPCALNPDAYVQLSNVLRTRHLDGSLVDPTDLPSRRALLG
                     ETVKGEQLMLTNAKGRDIIIEAAGIPLRVSGRLIGAVGVWNDITERTRIERERELLLA
                     SERAARCEAEHANRVKDEFVATLSHELRTPLNAILGWAQLLRRGRPDPEDVKEALEVI
                     QNNALAQKQLIDDLLDVSRMMSGKIRLNRRPLNVSQAAVEALASIRPQAEEKGLRLEE
                     AIEHEAGCVQADPERLQQILWNLLSNAVKFTPAGGQIRFALERQGQNAVLTVSDTGIG
                     IRPEFLPFLFQRFRQADASTTRRYRGLGLGLSLVKQLVDLHGGSISASSPGENQGTTF
                     TVTLPLITEPVMECPPAAADRGGGDGGEPDTTLQGLRVLIVDDLADARELLSRLFREY
                     AAAPVAVGSAAAALEQIRKAPPDVLVSDISMPDEDGYSLISKVRALPAERLARIPAIA
                     VTAFARPEDRSQAIRAGFQAHVSKPVDPAQLIALVAGLSGRTLHRKKA"
     gene            complement(15129..16556)
                     /locus_tag="PLU99_02820"
     CDS             complement(15129..16556)
                     /locus_tag="PLU99_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16019.1"
                     /translation="MTSAAFPEPAPQKLSWRFPRTFWVANGAELLERAAYYGMFIALA
                     LYLTERVGFSDFQTGIVAGCFASVLYLLPMFTGALADRIGFRQALMLAFALLTVGYFT
                     LGVSQQKPIVILALAIIMVGGAIVKPVISGTAAKCSDDAHRPRAFSIFYMVINIGAFS
                     GKSIAKPLRTELGLEYINYYAAIMALLAFIFVALLYRNVGGEGSNRSVREIVRGLGKV
                     FSNGRFMCLIVIVAGFWAIQGQLYASMPKYVLRLVGEHAAPEWLANINPLVVVLLVVP
                     ITHLVRRFKPASSIGIALGIVPLAALSIAVSPLLQARAGTQVDIFGWFSLHPITVMAV
                     CGIALLGLAECFLSPKYYEFASKQAPPGQVGMYMGYQSLTTFLAWGFAFLSSGYLLTR
                     FCPDPKTLPPEVYAQWQAATSTAAGASATPLPEAYAHAHYLWYVYAAVGVVAFAALLV
                     FKFITDRIDRRREQAAQEAEVKASA"
     gene            complement(16656..18827)
                     /locus_tag="PLU99_02825"
     CDS             complement(16656..18827)
                     /locus_tag="PLU99_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO16020.1"
                     /translation="MSTPNAALLRTLGAFFLLISITMSVMLALSTLGSLKLPGCGPGS
                     ACSDLSNGYWGSIRWSETGKWPVAYLGLAYFVAVLVAWLAAPGGIASLFRWLIRCGAV
                     VSVGFMMVMIKEWHFCNYCIGAHAGNIAFWIVAEATRWRQIPSLKPIAIFIVAFAVAT
                     GGLKAADARVRHEADQQDKRDLEASIAGLLQKTDAEMKAAQQPAPAPQSPADQGDKPV
                     TPAPDARAASSAQPPSAPPVGADSEFKVTIGRGEDARELPIRKIEPGETPQPPPQAAP
                     AQGAPAPAKSESGAAQSKPGERPDKPPPPPGWDWDTILSQDDPFKDGFCGLYVKGPKE
                     ASVRIVMFTDYQCPDCKRVEGEIQEVLKKYPNDVSLSVKMFPFCLECNPTVDRTLHPN
                     ACWAARAAHAAGILWGNDGFWKMHEWLFDRKGIFQTTRILEDGVREIGYDPQGFIRVV
                     TGPETLKRIETEIKEARWCGIYQTPMIFINGHEIRGWRTTNAVPRAVEALLQRRPPAR
                     DHSQDRPPIALDKMVGDWRENGRRQMPPDNPRHSRGPEGAPVQVIVYGDFEEEATAET
                     DIWFRDYVAKHSDTQYTWRAFPFNQECNRCMKFTRYEQGCWAARAAEAAGKLKGEEGY
                     WAIHDWLMRNQGSFNDEALRQAATELGFDADALLETMKSPEVEDAIKDDVNGADRVSL
                     QAIPLIIINGKQVGRWRNVPERKWEVLEAIIDEAAGRRPPR"
     gene            19308..19673
                     /locus_tag="PLU99_02830"
     CDS             19308..19673
                     /locus_tag="PLU99_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOO16021.1"
                     /translation="MACREKAHGGLGLQFRSESDGTLVAQFFCDPVNCSYPENVHGGH
                     LALLLDAAMGHCLFEHGIIAVTGRLNIGYGEPVRVGQPAQVRVAIHDHQEELYRVRGE
                     IVQAGRVCVRAEGKFTIRQ"
     gene            complement(19717..21435)
                     /locus_tag="PLU99_02835"
     CDS             complement(19717..21435)
                     /locus_tag="PLU99_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO16022.1"
                     /translation="MNPLRAENAGMSGAGGNDKRRAILILNTGAAGLQDIQAAIAGLG
                     EINYVETTEDALAALRTGNYDVVLSAPSDMFLLVRAEARQRGELVLDKLGQAVCIVDR
                     AGTLVWANTRLSTYPPEVVEAIRQHCGEICQEFAKERPPEHDNTPRRRTIRVGPGYFF
                     DLTVSPLPAPDRKVEEVVGLAIDISAAQRLQERMDTIDAAGRELVQLDAEALAQLDVC
                     ERLRVLEEKIHHYAHELLDFDNFAVLVIDQQTRHLETVIASGFSEEVRSMPLMASTEG
                     NGITGYVAATGRSRICPDVSKDPLYLPGLERAGSSLTVPLRLRDQVVGVFNVESQEIG
                     AFTEEDRRFAEIFARYVAIALHILKLLAVERSTTTGQITADVASELSAPLNDIIAEAT
                     ATINDYIGQDDLRARLRAIIDNVDRVKRTLTELTESRGVRGLIPETRAVDPLLAGRRI
                     LIADDEAIIRETISDVLTKNGALTVMARDGTEAVGMIQVQHFDLVVSDIKMPHKSGYE
                     VFAAARAANPDCPVILITGFGYDPDHSIVRASREGLAGVLFKPFKVDQLLEQIRRALS
                     KQTPAA"
     gene            complement(21887..22669)
                     /locus_tag="PLU99_02840"
     CDS             complement(21887..22669)
                     /locus_tag="PLU99_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOO16023.1"
                     /translation="MPSDEFSYRTAAEETWRIFRIMAEFVEGIDVLSRVGPAVSIFGS
                     ARARPEDRYYQQAVQLGAKLAHERFTVITGGGPGIMEAANKGAFEAGGKSIGLNIWLP
                     VEQEANAYQTISLDFNHFFVRKVMFVKYAVAIICFPGGFGTMDEFFESMTLVQTLKMR
                     AMPIVLVGSDFWNPLHDWVRDTMLGVYKAISPADLELCTVTDSVDEAVAIVLKRYEED
                     RSLAGQPKTEEEMQLTPHLRVTAEGTLKGSPPVGPGRERQTG"
BASE COUNT         4334 a         7384 c         7315 g         3833 t
ORIGIN      
        1 tttgacgtgg tgctgacgga cctgcgcatg cccggcatgg acggggtggg cttgttgcgc
       61 gagatgcggc ggctggggct ggacgtgccg gtcatattga tgaccgccta cgcttccgtg
      121 gcaaccgcgg tcgaagccat gaagctgggc gccttcgatt acatacaaaa gccgttcaat
      181 gcagacgaag tcgccatcct ggtcgaacgc gccgtgcgcg accggcaact ggtgcgcgac
      241 aacgaagtga tgcgccgcac gctcgaggac gtcgagtcgc agcgcaaact gatcggctca
      301 tcgccggcca tgcgcccggt gctggagaag ttgcagcgcg tagcgcagag ctcggccacg
      361 gtcctgatta tgggtgagag cggcacgggc aaggagctga ttgcgcgggc agtccacgcc
      421 gcttcgcccc gggccgcgca acctatgctg tgcgtcaact gtgccgcgct ctcgcccacg
      481 ctgctggaaa gcgagctgtt cgggcacgaa aagggcgcct ttaccggggc cgaccggatg
      541 cgcaaagggc gctttgagct ggccgacggc ggcacgctgc tgttggacga ggtttccgaa
      601 atcgccccgc cgttacaggc caagctgctg cgcgtccttc aggagcgcga gtttgagcgc
      661 gtcggcagct cggtaacgcg ccgcattgac gtacgcgtta tcgccaccac caaccgcgac
      721 ctgcgcgagt gggccgccaa ggcccgcttc cgcgaggacc tgtactaccg gctgagcgtg
      781 ctgccgatcg aggtgccgcc gctgcgcgag cggcgcgagg acttgccgct gctgctggat
      841 tacttcctcg gccgcatcaa ggtccgcgaa ggacgcgagc gccccacctt taccccggaa
      901 gcgatggccc tcttggccga gtactcttgg cccggaaatg tgcgcgaact tcaaaacctc
      961 tgtgaacgcc tggccgtttt ggaggccggg cgcgagatag gccccgatac ggtgcggccg
     1021 ctgctcattg gcccactgaa gacggccagc gcgccggccg aaggtctgcg ctacagggaa
     1081 ggtcacattc tggaagattc ggagcagcaa ctgattctga gcacgctcga acggtttaac
     1141 ggccatcgcg agaagaccgc ccgggcgctg gggattggat tgcgcacgtt gggcctgaag
     1201 ctcaagaagt ggcgtgaaga agggcggttt cccgagttgg tagtacaacg gaaggtgctc
     1261 taggttctgg cgtttgggta ttcacgaata tggcggcatg gcatttgcct tgcgtctgtt
     1321 gactcgcggc gtgagcggag cacgttgatg tggattgatg gcttgatcga cgggcgcacc
     1381 acgcgggcgg cggaactgac ggccagattc gccgaagccc gccataacgt gctggcccag
     1441 aacctcgcca acattgacac gcccgactac cacagccgga acctcgatcc gcgcctgttc
     1501 caggagtcgc tggcggccgc gctcgaacgc gcgacgcagt gcaatagcag ccggctggat
     1561 ttgcgcggca acgcgcagtt ttcgaccacg cccaccggcc aggccgaagt gcgccccgca
     1621 gtggagccgg ccccgaatgt gctttttcac gacggcacta acaaccggct ggaaggcctg
     1681 atcgccgacg cgaccgaaaa tgcgttgtac tacgagatga gcatcagttt tctgcggaac
     1741 agcttccaaa acgtgctcag tgccatccgg ggcagagtgg catgatcggc ggtgaagaca
     1801 aatgatcggc acacttgaca tcacacgctc ggccctgatc gctgagcgca cccggctgga
     1861 cgttatcgcc ggcaacattg ccaacgcgaa cgtcacgcgc caagccgacg gcacgatcct
     1921 gccgtatcgc cggcgcttcg ccacctttat gagcggcgac ggccggggcg gcccgggcgt
     1981 tcacgtcgac tccatcgagc aggatccttc ggacttcaag ctgcgctacg cgccgggaag
     2041 tccggacgcg attcccagcg gaccgctggc cggatacgtt caattgccga acgtcaacat
     2101 cacgatggaa tacgtggatg cgctcgaggc gagccgcgcc tacgaagcca acgtggcgat
     2161 gctgaacgtg accaagagca tgctgcagca ggcgatccgc cttttcgcgt gatgacgaat
     2221 gatcgaccca gtttcatcaa ggcccgttag cccaatttgc ccgccggcga ccagtgcccc
     2281 ggccacggcc cccggcgccg ccggcggcga ggcgagcttt gccgcgctgc tccgccggca
     2341 gttggagcag gtcagcgata tggataacca agccaagcaa ggcgttcagg atctgctgac
     2401 cggccgctcc cagaacatca ccgaggtgtt cgtggccgcc cgcaaggccg aggtcgcttt
     2461 cagcctgctc atggaaatcc gaaacaagct ctcggacgcg tacacggaga tcaagaacct
     2521 gcgcgtctga tgcggctgac agcatcgaat agatgatcgg cggccgagcc gtcgggagcc
     2581 ccgggaatgg agtccctgca aaaactgctg gcgcgggtct tcacgcaaac gcgcgacctc
     2641 tcgcgttcgc agcgactggc gatcttgctg ggtggactgc tggtcggcgt ttcgctgctg
     2701 ggcctgctgc gctgggccgc cacgcccgag atgacgccct tgctcgacca ggagctccag
     2761 ccggacgagc tggcccgcgt gcgcaccggt ctggagctga tcaaagagcc gttccggctg
     2821 gtcgggcaga aaattctggt tcgtgccgac gcgaaccgct ccgccatcct ggcgcaattg
     2881 caacagcagg acaagctgcc ctccgacacc agcgccggct tcagcgccct ggtgaaggaa
     2941 tccaatccct ggatatcgca agccgagcat gaacgccgct ggaccgtcgc tctgaaacag
     3001 gagctggaac aggtcctgcg gcagtttcgc ggcgtgaaat cggccagtgt gttcctgccg
     3061 ctcaaccagg agcggcggcg cttcgcgcaa caggaagccg ccgccactgc cagcatcacg
     3121 ctcgtcatgg ccggcggcga acctgtgggc cgcgacctcg ccctggcggc cgcccggctg
     3181 gtctgcggcg cggttcgcgg gctgcctttg cggaatatcg aggtgctcga tggcaacggc
     3241 gtcagcgcct tggactggga tagcgaaacg gcggctggca gcagcctcga tcgccagcgc
     3301 cgcaaggccg agcaggacat ccgcgccaaa atcatcgccc agttgcccga cccgaagagc
     3361 cgcgtggcgg tgcaggtcga actggagctg accaacaaga ccagcgaaat tcaagcgccc
     3421 accgacccga tcgaaacgcg ggtcgagacg accgcggaac gcaccgtccg cgcgcgtccg
     3481 cacggccagc cgggtgtggt gcccaatgtg ggcgtgtccg ccggcattcc cgcggcagac
     3541 gaaggcacca gcaaagagac cagcagcacc gaatacacgt ctggttaccg gcgcacccaa
     3601 gaggctactt cgcccggcgg aatgaaagaa gtctgggcgg cggtgaatct ctcgcgcagc
     3661 taccttgagc acgtgtttcg acagtcgcag ccgagtgcgg gcgagcccaa cgaggaacaa
     3721 ctgcagcagg ttttcgagcg cgagaagccg cgcgtcgtca gccaggttaa gaagctggtc
     3781 aaaccgcagg acgacgcgca cgtagcggtg gactggtact acgacacagt tctggaaacc
     3841 gagccggcgg tggtgcgggc cacggccggc gatgaagtgc tggactatgt gcggcggtat
     3901 ggggcgcaat ccggcctggg gctgttggcg gtgatctcgc tgggattgat gctgcgcatg
     3961 gcccgcaagc gcgacggggg cgaagccttc ggattggaga ttggattgcc caaggagtct
     4021 atcgaggcgg cgcggcgggc ggccaccgac gtggccaagg ccgcgcggcg cgagcagagc
     4081 cgccgcacgg ggcaggccgc ggcgcccggt caagcggcgg gcgaggcgct ggaagtgatg
     4141 ccttcggcga tcgggcaggc cgcgacgacc gaaggcgtgt tggtggcgca ggaagtcgac
     4201 gagcgcaccg tacagaccaa caagatgctc gaccaggtgg cccaggtcgt cgatggcgac
     4261 gccgaaatgg ccgcgacgtt actcgagcag tggattcagc gcaatgacgc cttggagcgg
     4321 tgagcggaac agatggcgcg cttcagtctg aaaacaccgg atgagccgtc cggcctgacc
     4381 ggcatccgca aggccgcgat tttgatgctg gcgttgcggc aggacacggc cgccaaaatt
     4441 ttggcggggc tgacgcgcga tcgggtcgag gaaatctcgc gcgaaatcgc cagcatcgac
     4501 atggtcgaac cggaactgcg cgagcaggtc gtaaacgaat tctacaacct ggtcctggcg
     4561 cggcgctatg tcgaccaggg cggcttggct tacgcccgca atctgctgat gaagacgctg
     4621 ccgagcgatg aagccaaacg catcatggcc acgatcgagc attcaattca ccagcagccg
     4681 ttcagcttct tgcagaaaac ggagaaggaa aacctaatca cgttccttca ggaggagcac
     4741 ccgcaaacga tcgccctggt gttgtcttat ctgccgcccg tgatggccag cgaggtaatg
     4801 accggcctgc caacggagcg gcagatcgag gtggttacgc gcatcgcgca aatggaccag
     4861 acctcgcccg acattatcaa agaggtcgaa cgcggcctgg aaaaacgttt ggccggcctg
     4921 gtctccgaac gcttcgagcg cgtcggcggg atccaggccg tggccgaaat tttgaatctg
     4981 gccggccgcg ccaccgaaaa ggccatcctg gaggggttag aggaaaacga gccgggccta
     5041 gtcgaggaga ttcgccggct gatgttcgtg ttcgaggaca tcatgcgtgt gaacgacaag
     5101 ggcattcagg cggtgctgaa ggaggtcgag aacgaagagc tggccttggc cctgcgcacg
     5161 gcgagcgagg agctcaagga aaaggtcttc agaaatatgt cagagcgcgc cgcgcagatc
     5221 atcaaggaag aaatggagtt catggggccg gtgcgcgtcg cggacgtcga gcaggctcag
     5281 cagaagatcg tggacatcgt tcgccgcctg gaggactccg gcgaagtgat cgtggccggc
     5341 aagggcggcg accgggacct gattgtgtaa acggtgtggc gcctgcgtct cgccgctgtc
     5401 tgagtaggtc accggcctga cgcctttgcc acagcagtgc cccgtgagtg catggctggc
     5461 ataattcgca aggaaacagt gacacgcacc gcggcgttct cctttgccga cctggaacaa
     5521 caggggcggg cgattatcga acgcgcccag gcggaggccc gcgaaattct ggcggcggcg
     5581 caacgccacg cgctgggact ggccgagcgc gagcgctgcc gggcgctcga ggagggacgc
     5641 gccgaggggc tcgccgaggg acgcgccgcc ggactcgaac aaattcgggc cgaagcccac
     5701 gcggccgcca tgcgcgaggc gcaagccgac gtgcaacgcc tcacgcagag cctgcaggct
     5761 gccttggctg agttcgacca gcacaagcgc agcctcatca ccgccgccga gagcgggcta
     5821 attgagttgg ccctgcgagt cgcgcgacgc gtctgcaagc tggaggccgg ccgctcgatc
     5881 ggcgtcgccc tggccaatgg ccgcgcgctg ctcgacatgg cccaacatgc agaagacccc
     5941 gaactgtgtt tcaatccggc cgatcttgaa atgctcaagg aaagcggggc tcagttggcc
     6001 gccgccgtcc agcagttctc gcacgtgaaa cttgtggctg acgaaaaggt cgaacgcggc
     6061 ggctgcctcc tgcggacacg cgcgggcgag atcgatgccg gactggaaac gcaagtgcag
     6121 cgcatcgccg aagccttgtg cgtcaccgtg gccgacgtcg aacccacgcc gccgcccgcg
     6181 ccatcgccgg agctgccggc caccaatgca agaccaccgc taacgaccga agggaacaca
     6241 gctacgtgac cgcggcggcc gcacaaaact ctctggccta cgaccgggcc cgtctgtcac
     6301 gaccgctcgc ggcagccgcc gccacggatg tgttcgcgca cgtcgcagac gaagtctcgg
     6361 ggctgctcag tgaacgcatc atcgggcggg tcgccgccat taccggcatg accgtctacg
     6421 ccgcagatct gcccgtacca gtcggcgcac gctgcgttat tcacacgcgc cgctccggcc
     6481 gcatttatgc cgaagccgtc ggcctgctca gcaatcgaac tgtgctttcc gtctttggcg
     6541 aagccacggg catcgccccc gacgacccgg tggaatgcct gtccgggccg ccgcgcgtcc
     6601 cggtcggctt cgacttgctt ggacgagtcc tcggcagtca gggtgaaccc ctggatgggc
     6661 gcccggcccc acgcctaaac attcgctacc cgctttactg cgatgctccg caggccctgc
     6721 agcgccgccc cattgaccag ccgctcggcc tgggcatccg ttcaattgac ggcctgctca
     6781 ccgccggtcg cgggcaacgc ctgggcatct tcgccggcac gggcgtgggc aaaagcgtgc
     6841 tgctgggcat gatctgcaaa cacacgcagg cggacgtgaa cgtgctggcg ctgataggcg
     6901 agcgcgggcg cgaggtgcgc gacttcgtcg aacgccagtt ggggcccgcc ggactggcac
     6961 gcagcgtact ggtagtctgc acgtccgacg gcgctccggc cttgcgcgtc cgtgccggct
     7021 tccatgccac cgccatcgcg gagttcttcc gcgaccaggg gctggacgtg ctcctgatga
     7081 tggattcgct aacccgactg gccatggcac agcgccagat cggcttggcc gccggcgagc
     7141 cgcccaccgc aaagggttat ccgcccagcg tttatgccct gctgccgcgc cttttggaac
     7201 gggccgggcg cacgcagtcc ggcagcatta ctggaatgta caccgtgctg gtcgaaggcg
     7261 atgatatcga cgaacccttg gccgacgtgg ttcgcggcat actcgacggg cacatatggc
     7321 tctcgcgcag cctggccaac cgcgggcact acccggccgt cgacgtccta aacagcatca
     7381 gccgcgtggc ggacgacgtg atcgacgccg agcagcgcag cgcggctctt gcggtccggc
     7441 gggtgctggc cacttggcac gacattgaag acctggtcag catcggcgcg tacgtcccgg
     7501 gcgccaatct cgattacgac gtggccgtgc agacgcgcgc ggccatcgaa gacttcttgc
     7561 ggcaagaccg cactagcggc tgcacgttcg cacaagccgc ggccgccctg aagcagttgg
     7621 gtcagctaat acagagcacg acgcagcagc tccaggcgcg ccgcccagcc ccggcagtcg
     7681 acgcaccgcg caggcccgcg caggacggcg cgcgcaagaa cactcggacg caaaatggga
     7741 aatgaggtat ttcttgatgt ggagcacatt ctttcaatac taacaactaa taactaacaa
     7801 ctaatgacca aggactaaag actaacgact aaggactaac ctccgcgcac aagcggcccc
     7861 gaaggatacg aaaggccata tgcggcgctt caaattcccg ctgcagacac tactaaaggt
     7921 gcgcaagcta cgcgaacgcg aggcaaagcg caaagtcgcc gcccaacgcg cggatattgc
     7981 gcggctcgac cggctcaacg aggccacctg ggccgaaatt tccgcccagc acgccggcct
     8041 actgcagaac cagcagcagc cctttatcaa gcccgcggac ctggcgcgca cccggacctg
     8101 gatcgctcat ttgcgggcca ctatcgccca gcgcacgctg ctgcgggccg aaatgacggc
     8161 tcgtctgcag caattgcagt ccgagtacaa cgcggcccgg caggaggcgc gcattattga
     8221 gaagttgcgg cagcgccgtt gggacgagta ccgccgccgg cgcgcccgca aggagcagag
     8281 cgcggccgat gaattggcac aacagttgca gactaaagcg gcgggcgact ccgccggccc
     8341 ccgccccaca gcggcggtcc catagttgtt gtcgcagaat cgtgcggctc gttgctggat
     8401 cgtgtgatta atagtggccg ggtatgaact ctggcaccgt ggcgaatagt acgtgttaag
     8461 cagactgtac aacctgctgt cgactgtatc aatcgcgacc ctgctggccg gcggcggctt
     8521 ttcggccttt ctgtttggca cgggccgctt gaacgcggcg cgcctcgaac ggatcggcgc
     8581 cgtcgtacgc ggcgagctcg acgaattccc acagtcgcag cccgccactc agccggcaga
     8641 actcgacgag ccgcctgtag gccgcgacca gtcgctggag caaatagatg ccgctcggcg
     8701 gcagcggcgg ctgcaatcgc acttgctgga acgcgccgcc cgcgacatcg aggctcagag
     8761 acgactgctc gaccagtcac tgcaatacct ggtgaacgag cgtgagcaac ttgcggctca
     8821 gcaggccgat tgggccgccg agcagcagaa ggtcacgggt gaggtggtaa atgaggccgc
     8881 ccagaagcat ttggaactgt ttgccgggct ggccccgcag caggccaagg agcacctggt
     8941 gtggctctgg agcacgaata aagccgaagc gctcgccctc gtcatgcagc tcgaggtcgc
     9001 cacaaccaag cgcattctgg cgcaaatgaa ggcgcctgag gaacagaaga tattatccga
     9061 actgctggag cagctacgta cctatgaagc cagtggcgct gccaccatgt caaggaagac
     9121 tgccggcgac gccgcaccct agaggtgccg catgcagttt agtccggtcg ctccaactgt
     9181 gcccgccccc atcgcgggac acgcggggct tctgcttctg ccccaccttc tcaggctcac
     9241 cagcctgagc ccgcaaacgc tagcgctgcg cgaacaacat caggccgaac gcgaggagga
     9301 tgatcgtcgc cagcgcgacg cgaatgccgc ggcccagcag acggccgccg cagccgctgc
     9361 cgacgcccgt cccacgttcg ccgagattac ggcagaagcc gaacgcgaaa tggcgccggg
     9421 cagtcgtgcc gcccagcgtc agatggccaa cgaggagcaa cagaaggcca cgctgcaacg
     9481 tgacaacttc cagcgcacgc tggccgacgc ggccgctcgc gagaacgcga gcgccagccg
     9541 cgccagtgcg ccttccactg cccaggccga ggccgaaagc gagccgcacg gagcggcggc
     9601 gccccagagc gcagccgcag cgaagccggc accggccgca accgcccagc ctaaccagcc
     9661 cggcggctcg atcccggtcg ttccggccga tagagcaggg ctagccccgc cgcaaaccaa
     9721 tggtgtcgca accggcgcac aacaaatttc agctagcgcg atttcggccg ccgcgcccgc
     9781 taactcaaat gcggtcaaag cccttggggc cttgccccca tctccggcca caactgcacc
     9841 cccggcgggc gacgcgctca cgccatcagc gccggccaac tccgccgcgg gcgcctcaaa
     9901 ggcggcccag ccggcggcgg ctacgcaaaa cgttactgcc acagtcagtg ctccgggcac
     9961 acaaacaaac ccggcgcgcc cggctatgtc gaactctgga cctgaagccg cttcgcccca
    10021 gaccggcaag aatgacgcaa acttcgagcg aatcctgcgc gtgctacgcg cacagatcag
    10081 ccacaatcgt tcacagacga cactacaact cgacccgccg gaactcggga aaatacggct
    10141 gcatttgggg ctgcgcgacg atactttgac actgcgggtc gagcctcaga cgcaagtggc
    10201 atatcgcttg ctttacgaac atctggattc gctgcgtgac ggactgcacg cgagtggcgt
    10261 ccatctggag cacgtcgaaa tccgtccgcc cgcagcgcag gccgcgccgc aggcctttga
    10321 cctgccgcca cactccggcg gcggaggaac cggcggcggc gcaggagcgg agacggatca
    10381 tccgcaacac tcgggaacgg attccccggc ggcgggcccg tcacagaatg cggccctcga
    10441 cgaagattcg gaaccgtcgg cggagccgcg ggttaacatc tgggcgtaag cagccaggga
    10501 gggcggcttg cgcaggcggc agcgcagggc gctggccgga gcgtgggtct gactatgcaa
    10561 accaatgcca tcaacggcgt caccagcgcc aacagcggtt catcctcgag tgccggtctg
    10621 aatcgcatga ccagcgagga tttctacaat ctgcttgtga ccgaactgca acagcaggat
    10681 ccgttggagc ctaccaaaac ctccgacatg attgggcagg tctcgcaaat ccgctcaatc
    10741 gagcagtcgg cccagctcac gcagacattg caacagctcg cgcagcagca gcgcaccgcg
    10801 ggggccggcg acctgctcgg aaagtacgtt gagggcgtcg gtctcaacgt cggtggggaa
    10861 ccggccttgc tgtcgggtgt ggttatcgcc atccgcttca tggctgacgg cactgcggtc
    10921 ctcgagctgg acaacggcgc catgctcagt gccaacaacc tcaccaaagt gaccgaatgc
    10981 gctgatgacg cgggagccac ggacgcggcg aacacggcca acacgaccaa tacgaacgcg
    11041 gccgcggccg ccaaacgccg gacgccgagc agtgagaacg cctactcgaa cacgatacag
    11101 tcgtggctgg acagtctgct gcacccgcag ccgcgcaccg ccacctcact ccagaagtaa
    11161 agcacaagcc accaagggga gctttccatg ggtctgacga ccgccatgta tacagggctg
    11221 accgggatca atgccaacca aacccggatc aataccatag gtaataacat cgcgaatgtg
    11281 aatacgaccg ccttcaaggg ctcgcggacg ctgttccagt cgcagttggc gcacaccatg
    11341 tccctgggca ccgcgcccag cgccaccact ggcggcacga accccattca aatcggtttg
    11401 ggcgtggacg tcggcgccac gcaacgcaac ttcacgcccg gttcgctcga aaccaccggc
    11461 atcgccagcg acctggccat cgacggacgt ggcttcttcg tgttacgcga cgccggcggc
    11521 gggcaagtct acacgcgcga cggctcattt gcgctggacg cgcagaaccg cctggtcacc
    11581 accgacggca agttcgtcca gggcttcggc gtcgacagcg gcttcaacat cgtgcccggc
    11641 gtcctgaccg acatcaccat cccgctgggt acggcctcaa tggcgcggcc gacctcaacc
    11701 gtgatcatgg acggggacct gagcgcggcc ggcaccgtag ccacgcgcgg ctctgagcat
    11761 ttgtcgcagg cgctggtcga cggtgcgggt aacgccgcga cggcgggtac ggccctcacg
    11821 aacttgttct ccgccgccga cccgggcacc gccctgtttg ccacgggcaa taccattagg
    11881 gtctcgggcg tcgtcaaggg cgagcgcgag ttgcccgccc gctcgttcgt ggtcggcgtt
    11941 gacggcacga cgctgggcga tttcgcgaac tggctgcaag gcgctctggg catcaatacc
    12001 gacgccggcc tgcccggcac gcccggcgta acgattgaaa acggcgcgct cgttatccgc
    12061 ggcaatgccg gcgagcaaaa cagcttcgaa atctccggca acgacttcgc cagtgacaac
    12121 gccacggttt cactgccgtt ccagtttgct caaacggctg ccgccgatgg ctccggcgtg
    12181 ttcacgtcct tcactgccta cgactcgctc ggcacgccgg tgaccgtcaa cgtgacattt
    12241 gccctcgaac ggacacccaa cggcgggccg gtctggcgct actacgcgga gtcgccggac
    12301 gccagggacg gctcacgcgt gctcggcacc ggaacggtag cgtttgacaa cgaaggcaac
    12361 ttcgtcggcg ccacgggcaa ccagttcaca ctcagccgcg acagcacggg cgccgctacc
    12421 ccgctgacgt tcacccttga cttctccggc gtaaacgggc tctcgaccca cacctcgagc
    12481 atcgtcgcca gcgagcagga cggtttcccg cccggcaccc tggtgaactt cggcgtgggc
    12541 gaggacggca tgatctatgg cactttcacg aacggcctga cgcaaaggct gggccaagtc
    12601 gcagtggcca cgtttgccaa cgaggaagga ctggtcgggc tcgacgacaa ctgctatgga
    12661 gtcggacccg cttccgggaa tgccacgatc ggcgcccccg gacaacttgg cgccggcacg
    12721 gttctgagcg gcgcgctgga gctttccaac gtcgacttgt cgcgcgaatt catcggtttg
    12781 gtgacgtcca gtaccgggtt ccaggcctcc agccgcgtga tcagcgtctc aagcgatttg
    12841 ttgaatcaac tgctgatgat catcagatag cgcgccgcgc gtgcctccca gagcgcctca
    12901 gcggaaattt gcgctctcca gcggttcgcc ctggtgctag gctttcttgc ggtggagcgt
    12961 tcgcccggat agaccggcca ctaacgctat cagttgggcc gggtccaccg gcttgctcac
    13021 gtgggcctgg aacccggcgc gaatcgcttg gctgcggtct tcggggcgcg caaacgcggt
    13081 cacggcgatg gccggaatgc gggctagacg ctccgccggc aaagcccgca ctttgctgat
    13141 caggctgtac ccgtcttcgt ccggcatgct gatgtcgctg accagaacgt cgggcggggc
    13201 cttgcgaatc tgctcgaggg cggccgctgc cgagccgacc gcaaccggcg cggccgcgta
    13261 ttcacggaac aatcgcgaaa gcagttcgcg cgcgtccgcc aggtcgtcca cgatcaacac
    13321 ccgcaggccc tgcaacgtcg tatccggttc gccgccgtcg ccgccacccc tatcggctgc
    13381 ggcggggggg cattccataa cgggctctgt tatgagcggg agcgtcacgg taaaggtggt
    13441 gccctggttt tcaccggggc tgctggccga aatgctcccg ccgtgcaggt cgaccagttg
    13501 cttgacgagc gacaggccca ggcccagccc gcgatagcgg cgcgtcgtgg aagcgtcagc
    13561 ctggcggaag cgttggaaga ggaaaggcaa aaactcgggg cgaatcccga tgcccgtatc
    13621 gctcacggtc aaaacggcgt tttgcccctg ccgttccagt gcgaagcgga tttggccgcc
    13681 ggccggcgta aacttcaccg cgttcgacag cagattccac agtatttgct gcaggcgctc
    13741 cgggtcggcc tggacgcagc cggcttcatg ctcgatagct tcttccaggc gcaggccctt
    13801 ttcctcggcc tgtgggcgga tggaggccag cgcctcaacc gcggcttggc ttacgttgag
    13861 cgggcgccgg ttcaggcgga tcttgcccga catcatccgg cttacgtcca gcaggtcgtc
    13921 gatgagctgt ttctgcgcca gcgcgttgtt ctggatgacc tccagagctt ccttcacgtc
    13981 ctccggatcg ggccgcccac gccgcaacag ttgggcccag ccgaggatgg cgttcaacgg
    14041 cgtgcgcagc tcgtgcgaaa gcgtcgcgac gaactcgtct ttgacgcggt tggcgtgctc
    14101 ggcctcgcag cgcgccgcac gctcgctggc caagagcagt tcgcgctcgc gctcgatgcg
    14161 ggtgcgctcc gtgatgtcgt tccagacgcc gaccgccccg atcaagcggc cggacacgcg
    14221 cagcggaatt ccggctgcct cgatgatgat gtcgcggccc ttggcgttgg taagcatgag
    14281 ttgctcgcct ttgaccgtct cgcccagtag cgcccgccgc gacggcaagt cggtcgggtc
    14341 gaccagcgaa ccgtccaggt gccgcgtccg aagcacgttt gagagctgca cgtaagcgtc
    14401 cggattcagg gcgcaggggt cggctgccag cagctcatgc gcagcgcgat tcgcacgcac
    14461 cggctttccc aggtcgttga acaccagcac cggctggttg agactgtcga aaatcgcttt
    14521 caactcagcg gcccgcgcct cggattcggc gagcgagtcg gccagcgtgc ggctatagtg
    14581 cttatgctgc tccagtaggc ggaagcgcgt gttccacaga attctgccga tggcggaaac
    14641 aatccaaatc gccgcgattg ccagcgcgcg attgtggact tctatccagt tgagcaacgg
    14701 cgcggggggg ctgacgaaat gaccgagcac gatcaggccc gaggcagtca cggccatcag
    14761 ccagtagtat cgcggcccac cccacgccac tgttaccagc aacagcggca agtagcccag
    14821 cagcaccacc aggcccagcg gaacttgtgt gtcaatcagg aaaagcagta gcgccagcgc
    14881 gaggacgaac gcaaacagca ctccggcgcg gccgcgcggt gctgtattaa gcccctcatc
    14941 cttgtgtccc gccacttgga ctcctcgctt caggccggat tactacgatt tgtcagccgg
    15001 atgcacggct cgggaatgtt ttgttatccg cggctccatc cttcctttta gctaagactg
    15061 agtgccccga aaagatattt caagttaaaa acatgtaagg gcgtgtcgga attgagctta
    15121 acgccttgtc aggcggacgc tttgacctcc gcctcttgcg cggcttgctc gcggcggcga
    15181 tcaatcctgt cagtgataaa tttgaagacg agcagcgccg cgaacgccac cacgcccacc
    15241 gcggcgtaga cgtaccagag atagtgagca tgggcgtagg cttcggggag gggggtggcc
    15301 gaggcgcccg ccgcggtcga cgttgcggcc tgccactgcg cataaacttc gggcggcagc
    15361 gtcttcggat ccgggcagaa gcgcgtcagg agatagccgg agctgagaaa agcgaagccc
    15421 caggcgagga aagtggtcaa gctctgataa cccatgtaca tgccgacctg cccgggcggc
    15481 gcctgcttgg aggcgaactc gtagtacttg ggcgaaagga agcactctgc caggccgagc
    15541 agcgcgattc cgcagacggc catgacggtg atcgggtgca gagagaacca gccaaatatg
    15601 tccacttgag tgccggcgcg cgcctgcagg agcggactga ccgctattga gagtgccgcc
    15661 agcggcacga tgccgagggc gatgcctatg gagctggcgg gtttgaagcg ccgcaccagg
    15721 tgcgtgatcg gcacgaccag cagcaccaca accagcggat ttatgttggc cagccactcc
    15781 ggagccgcgt gctcgccgac cagtcgcagg acgtacttcg gcatcgaggc atacaactgc
    15841 ccctgtatgg cccagaaacc ggcgacaatg acgatcaggc acatgaagcg cccgttcgag
    15901 aagactttgc ccagcccgcg cacgatttcg cgaacgctgc ggttggagcc ctcgccgccc
    15961 acgttgcggt agagcagcgc cacaaatatg aacgccagca gcgccatgat cgcggcatag
    16021 tagttgatgt actccaggcc caactcggtg cgtaacggct tggcgatcga cttgcctgaa
    16081 aacgcgccga tgttgatcac catgtaaaaa atcgagaacg cccgcgggcg atgtgcgtcg
    16141 tccgagcact tggcggccgt gcccgagatg accggcttca cgatggcgcc gccgaccatg
    16201 atgatggcca gggccaaaat cacgatgggc ttttgttgag agacgcccag agtgaagtag
    16261 ccgaccgtga gcagcgcgaa ggccagcatg agggcttggc gaaagccgat ccgatccgcc
    16321 agcgccccgg tgaacatcgg caacaggtag agcaccgagg cgaagcagcc ggccacaatg
    16381 ccggtttgga agtcggagaa gcccacgcgc tccgtcaggt agagcgccag cgcgatgaac
    16441 atcccgtagt acgcggcgcg ttccagcaac tcggcgccgt tggcaaccca gaaagtgcgc
    16501 ggaaagcgcc acgacagttt ctgaggcgcc ggctcgggga atgccgctga agtcacaaga
    16561 ccatcctctc aacgattaat acagcggcgc accgcgcgcg gtgcgccgct gaactcgctt
    16621 caaacttcac acgccgccag gctcacacgc cgacattagc gcggcggccg gcggcccgcg
    16681 gcttcgtcga tgatcgcctc gagcacttcc catttgcgct cgggcacatt ccgccaacgt
    16741 ccgacctgct tgccgttgat gatgatcaac ggaatcgcct ggagcgatac gcggtcggct
    16801 ccgttcacgt cgtccttgat ggcgtcctct acttccgggc tcttcatggt ttccagcaac
    16861 gcgtcggcgt cgaagcccaa ctccgtggcg gcctgccgca acgcctcgtc gttgaacgag
    16921 ccttggttcc gcatcagcca gtcatggatc gcccaataac cctcctcgcc cttcagctta
    16981 ccggccgctt cagccgcccg ggccgcccag cagccttgct cgtagcgcgt gaatttcatg
    17041 cagcggttgc actcctggtt gaacgggaac gcccgccacg tgtactgcgt gtccgagtgc
    17101 ttcgccacgt agtcccggaa ccagatgtcg gtttcggccg tcgcctcttc ctcgaagtcg
    17161 ccgtacacaa ttacttgtac gggcgcgccc tcgggcccgc gtgagtggcg cgggttatcc
    17221 ggcggcatct ggcgccggcc gttttcgcgc cagtcgccga ccatcttgtc cagcgcaatg
    17281 ggcgggcggt cctgcgaatg gtcacgcgcg ggcgggcgac gctgcagcag agcttcgact
    17341 gcacgcggca cggcgttggt cgttcgccag ccgcgaattt cgtgcccgtt gatgaagatc
    17401 atcggggttt gatagatgcc gcaccagcgg gcttccttaa tctcggtttc gatccgcttg
    17461 agggtctcgg gcccggtaac gaccctgatg aagccctgcg ggtcgtagcc tatctcacgc
    17521 actccgtctt cgagtatcct ggtggtttga aatatccctt tgcgatcgaa caaccactcg
    17581 tgcattttcc agaagccgtc gttgccccaa agaatgccgg cggcgtgagc ggcgcgcgcc
    17641 gcccaacagg cgttgggatg cagcgtgcgg tcgaccgtcg ggttgcattc caggcagaac
    17701 gggaacatct tgactgaaag cgaaacgtca ttcgggtact tcttgagcac ttcctgaatt
    17761 tcgccttcaa ctctcttgca gtccgggcac tggtaatccg tgaacatcac gatgcgaacg
    17821 gacgcctcct tcggaccctt cacgtacagc ccgcaaaaac cgtccttgaa tggatcgtcc
    17881 tggctgagaa tggtgtccca gtcccagccg ggcggcggcg gaggtttgtc cggccgttca
    17941 ccgggtttac tctgggccgc accagactcg ctctttgcgg gcgccggcgc gccctgagcg
    18001 ggagcagcct ggggaggcgg ctgaggagtc tcgcctggct ctattttgcg aatcgggagt
    18061 tccctggcat cttctccgcg cccgatggtt accttgaatt cggagtctgc gccaactggc
    18121 ggagctgagg ggggctgtgc agaggacgcg gctcgtgcgt cgggagccgg cgtcaccggt
    18181 ttgtcgcctt ggtccgccgg ggattgcggc gcgggggccg gctgttgcgc ggccttcatt
    18241 tcggcatccg ttttctgcaa caggcccgcg atggacgcct ccaagtcacg cttgtcctgc
    18301 tgatcggcct cgtggcggac gcgcgcgtcg gctgctttca gcccgcccgt ggcgaccgca
    18361 aacgccacaa tgaagatggc gatgggtttc agcgatggaa tctggcgcca gcgggtcgct
    18421 tcagccacga tccaaaaggc gatgttgccc gcgtgagcac cgatgcagta gttgcagaaa
    18481 tgccactcct tgatcatcac catcatgaag cccaccgaaa ccaccgcgcc gcagcggatc
    18541 aaccaccgga aaaggctcgc gatgccgccc ggcgccgcca gccaggccac cagtactgcg
    18601 acgaagtagg ccagcccaag gtaggccacc ggccactttc ccgtttcgct ccagcggatg
    18661 ctgccccaat agccgttcga caggtcggag caggcgctgc ccggtccgca acccggcagt
    18721 ttcagggagc cgagcgttga aagcgccagc atgaccgaca ttgtgattga gatcagcaga
    18781 aagaacgctc cgagagtccg cagcaatgcg gcattggggg tgctcaatgt ctggactgct
    18841 gcaccggctg aacgggtgtc agatccgatt tgagcctgct gtgatctgtt tttaggcatg
    18901 tcactcctca accagtcgga agggccgtag tttagccaaa aacgcggacc gcacaaacgc
    18961 ccggagcagg tcggaataag ctctcgcgcg atggtccgcc gaccgggaac ccgcggacgg
    19021 caactctgag ttgcactcgg acgtgccgca gccggcccgg cgcgttacta tttagattga
    19081 caacttcgtc atcagcttgg taggcggcgg cgtgcccgaa atgagccatt attttcgccg
    19141 cgcggcagtt ttgcgcccgg gaaaccagga tgagcaagcg acctcgccaa aacacgaccc
    19201 gaggaacgcg ccggggcgca accaagctac gcggctgcgc tgaccccacc gagttatcgg
    19261 ctcctgcgcc cggccagtcg ggccacactt cctgccactc ggcgtgcgtg gcatgtcgcg
    19321 aaaaggcgca cggcggcctg gggctacaat tccggtccga atcggatggc acgctggtgg
    19381 cgcagttctt ctgcgatcct gttaattgca gctatcccga aaacgtgcac ggcggacatc
    19441 tcgcgttgct cctcgatgcg gcgatggggc attgcttgtt tgagcacgga atcatcgccg
    19501 tgaccgggcg gctgaacatc gggtacggcg agccggtgcg cgtggggcag ccagcacagg
    19561 tccgcgtcgc catccacgat caccaggaag agctgtaccg ggtccggggc gaaatcgtgc
    19621 aggccggccg cgtgtgcgtg cgggccgagg gaaaattcac cattcggcaa tgagccggcc
    19681 gaacgccaag cctcaaatct cgaaactcga agccccctac gcggctggcg tctgcttcga
    19741 aagcgctcgg cgaatctgct caaggagctg atcgaccttg aaaggtttga acagtacgcc
    19801 cgccaaacct tcccgactgg cgcgaacgat cgaatgatcc ggatcgtagc cgaatccggt
    19861 aatcaggatc accgggcaat cggggttggc tgcgcgggct gcggcgaaca cctcgtaccc
    19921 gctcttgtgc ggcatcttga tgtccgacac taccaagtcg aaatgctgaa cctggatcat
    19981 gccgacggct tcagtgccgt cgcgggccat cacggtcagc gccccgttct tggtcagcac
    20041 atcggaaatc gtctcgcgaa tgatcgcctc atcgtcggcg atcaggatgc gcctgccggc
    20101 cagcagcgga tcgacggctc gcgtctcggg gataaggccg cgtacgccgc gggattccgt
    20161 aagctcggtt agcgtacgct tgacccggtc cacgttgtcg atgatcgccc gcagccgggc
    20221 gcgcaggtcg tcctgcccaa tgtagtcatt gatcgtcgcc gtcgcctccg caatgatgtc
    20281 gttcagcggc gcggacagct ccgaagcgac gtcggccgtg atctggccgg tggtcgtgga
    20341 gcgttcgacc gcgagcagct tcaggatgtg cagggcgatg gcgacgtagc gggcgaaaat
    20401 ctcggcgaaa cggcggtctt cctcagtgaa tgccccgatt tcctgggatt cgacgttgaa
    20461 gactccgacc acctggtccc gcagccgcaa cggtacggtt agtgaggagc cggcccgctc
    20521 cagaccgggc aggtacagcg ggtccttgct gacgtccggg cagatgcgcg agcgccccgt
    20581 ggccgcgacg tagcccgtga ttccattgcc ctcggtagag gccatcaggg gcatggatct
    20641 gacttcttcg gaaaagccgc tggcgatgac ggtttccagg tggcgggtct gctggtcgat
    20701 aacgagcacg gcgaagttat cgaaatcgag cagttcgtgg gcgtagtggt gtatcttctc
    20761 ttcaagcact cgcagccgtt cgcagacgtc gagttgcgcc agagcttcgg catcgagctg
    20821 aaccagttcg cgcccggcgg cgtctatcgt gtccattcgc tcctgaagac gctgggcggc
    20881 cgagatgtcg atggccaggc cgaccacttc ttcaaccttg cggtcaggcg ccggcagggg
    20941 cgaaaccgtc aggtcaaaga agtacccggg gccgacgcga atcgtccggc gccggggggt
    21001 gttgtcgtgc tccggcggcc gctccttggc gaactcctgg cagatctcgc cgcagtgttg
    21061 gcgaatcgcc tccaccactt cgggcggata ggtcgacagg cgtgtattag cccagaccag
    21121 cgttccggcg cggtcgacga tgcatacggc ctggccaagc ttgtccagga cgagttcgcc
    21181 ccgttgccga gcctccgcgc gaactagtaa gaacatgtcg gacggagcgc tgagcaccac
    21241 gtcgtagttg ccggtgcgca gagcggccag ggcgtcctcg gttgtctcga cgtaattaat
    21301 ttcgcccagg ccggcgatcg ccgcttgaat atcctgcaat ccggcggcgc cggtgtttag
    21361 gatgagtatc gcgcggcgtt tgtcgttgcc gccggctccg gacattcccg cgttttcggc
    21421 ccgcaagggg ttcatcgctg cctcgaagtc agcccagcat gataccccaa tacgctggca
    21481 gacacaagcc ctgcggggga ggagcgcgac ccggcgccgg agagcagagc cggggcgtgc
    21541 cgccccggaa tgcccgccaa caacccccta ttcgcgcagc cgcctgcccg ccttcggcgt
    21601 ctccaggatc cgaccccgcc tgatccgagc ccgaagcgcc agcgagggca cttgtctgat
    21661 cccagcccca ctgccaacca aacgcatgtc ctgggcgtgc gacccagcca attggcagca
    21721 ctccggtcaa atcccggctc gacacgagcc ggctctgcta ctcattgcaa acgtcgggcg
    21781 ctgaggacca ttgcagccgg ctgcggtatt ctggctgaaa gctgagagcc tatagctgaa
    21841 agctgaaccg cccagagcag ttcgccatca caagcacagg cccgatttaa ccggtctgcc
    21901 gttcacgccc cgggccgacc ggcgggctgc ctttcagcgt gccttcggcc gtcacacgca
    21961 agtgtggcgt gagctgcatt tcctcttccg tcttcggctg tccggccagt gagcggtctt
    22021 cttcgtagcg tttcagtact atggccacgg cctcatcgac gctgtcggtg accgtacaca
    22081 gctcgaggtc ggctggcgaa atggctttgt aaacgccgag catggtgtcg cgcacccagt
    22141 cgtgcagcgg gttccagaag tcgctgccca ccagcacgat gggcatggcg cgcatcttga
    22201 gcgtttgcac cagagtcatg gattcgaaaa actcgtccat cgtgccgaag ccgcccggga
    22261 agcagatgat cgcaacggcg tacttcacga acatgacctt gcggacgaag aagtggttga
    22321 agtccagcga gatcgtctga tacgcgttcg cctcctgctc caccggcagc cagatgttca
    22381 gcccgatgct cttgccgccg gcttcgaagg cgcccttgtt ggcggcttcc atgatgcccg
    22441 gcccgccgcc ggtgattacc gtgaatcgct cgtgcgccag cttcgcgccg agttgaaccg
    22501 cctgttggta atagcgatcc tcaggccgcg cccgcgctga tccaaagatg gagacagccg
    22561 ggccgacgcg cgacaatacg tcaatgcctt ccacgaactc cgccatgatg cggaagatgc
    22621 gccaggtttc ttctgcggcc gtacggtaag agaactcatc actcggcata gcgtcccttt
    22681 cggtgctggt gacgccatga tagctgatac gcggcgtacg tgcaggcaga agcgcggcgg
    22741 gagttgagaa ggcggaacgt ggggggcgtg tagtcgcgag ccggggtggg tccgcggcgg
    22801 tgccatgcca gcgtcagcgc tcgcgcatgg cgacccgcgt ttcacgatcg gacttatacc
    22861 ccgcgt
//
LOCUS       DAOSYS010000026        22146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1928_length_22146_cov_5.756417, whole genome shotgun sequence.
ACCESSION   DAOSYS010000026 DAOSYS010000000
VERSION     DAOSYS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22146
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1928_length_22146_cov_5.756417"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PLU99_02845"
     CDS             <1..1283
                     /locus_tag="PLU99_02845"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOO16024.1"
                     /translation="LNGAVTVNVPDVRAALARLAVRWYGLDDGPASDLRLIGITGTNG
                     KTTTAIMTRAIIQAAGYKCGLLGTVRYDLCGRSITAKNTTPGALELAAYLRECADAGA
                     RYAVMEVSSHALDQQRAAGLHFAAAGFTNLSGDHLDYHGTLENYAAAKARLFAGLDEK
                     AVAVLNRDDASSAIMVRDCRARTIWYSLQGPADITGSISRTTIRGTYYRMCVGGADLV
                     LENAVVGRHNVYNALTAAGLARAVGLPLEAIEAGLSSVRNIPGRLQRVPCVSGADVFV
                     DYAHTDDALRNVLSVLKPLVQGQLILVFGCGGDRDRTKRPRMAQAAGELADRIIVTSD
                     NPRSEDPQAIIAEIMLGFGQQVSEAVVTEPDREAAIRLALKTAGPGDVVLIAGKGHED
                     YQIIGNRRIHFDDVEVTIRAAEQLARAATETAKK"
     gene            1280..2704
                     /gene="murF"
                     /locus_tag="PLU99_02850"
     CDS             1280..2704
                     /gene="murF"
                     /locus_tag="PLU99_02850"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOO16025.1"
                     /translation="MITLILSEVVEAIGGQPAGELPTLSVRRVSTDSRSSAEGDLFFA
                     IRGPRFDGHDFIADALRGGAVAAVIAADRLTDVRQQIAGITSRVVPPGAIICVPDPVV
                     ALGRFAAHHRRLMSANVIAVVGSNGKTTTKAMIHHILSERWKGQCSPKSFNNEIGVPL
                     TLLSCEPADEYLVVEVGTNAPGEVAALAELIHPDMAVLTCISEEHLERLGDLNGVAAE
                     ECSILHKLEPGGFAAVNVDAPVVRRHLPDGGVTVATFGWNSEADVRLTDARYEAPWLH
                     LTINGRFKYRLPLAGLHNAVNAAGAITLARRLGMEHKDIAARLESFVSPPMRTEMLEF
                     GGITLVNDAYNANPGSALAALDTLSSLACQGRRVVVFGEMRELGEHAAELHQQVGEHL
                     RNGKFDLVLLVGRAGELMPNLKEDGQLFGPRILRCADVEACLERLAPELRRGDVVLLK
                     ASRAVGLDRLVAPLRERLASATVA"
     gene            2709..3854
                     /gene="mraY"
                     /locus_tag="PLU99_02855"
     CDS             2709..3854
                     /gene="mraY"
                     /locus_tag="PLU99_02855"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOO16026.1"
                     /translation="MLYLLLHWLTGGKGYAHEDPLFRATMAILLAFLIVWALGPRVIS
                     ALRRFKIGDRAEFDHATLNRMMADKRDTPTMGGILIVIAILVCVLLLGNLQSFYIRMG
                     LFCLVWLALVGGVDDALKLVSARRGSSRDGLKLWEKLMFQVALGALLAIFILRAGQTN
                     AEVARFTDTQMPYEHAYNLFNIPFWKQPIPLPTWIFVILAVIVLAGTSNAVNLTDGMD
                     GLASGCMVMTAFAFMILAYVIGDSNTAKYLLYPPIPGAGELAVMCGAAVGACLGFLWY
                     NSYPAQVFMGDTGSLPLGGLMGYVAIVTRLELMLFIVGGIFVIEGASVLIQIFYYKFT
                     GGGRFFRCAPIHHHFHLGGWSETQVVMRFWLVAALFAAFALATIKLR"
     gene            3857..>5082
                     /locus_tag="PLU99_02860"
     CDS             3857..>5082
                     /locus_tag="PLU99_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOO16027.1"
                     /translation="MGTIRHSVITDATPAPQEQLGRPYDSGVLLLASALMTFGVAMVY
                     SASMTVRGAQLDWHQWWHTPLRQCVFAAGGFLVMLFTAHCDYRWLASTRKGGLVRVGV
                     LYLIAIATLIAVQLVGREALGAQRALVVLTSPFTLSFQPAELAKVVMVIWLAVLLARM
                     HARAEEQRTRGYSARDLGIRNFTRGFLPTVISSGLLIGLTGVEDFGTAALMGVVMVMM
                     LVIAGARWRHLMLLALIGLMGGVGLIAMKPYRLERLKTYVYFLDESKPAPDPQKEGYQ
                     INQSMLAIGSGGWFGRGLGAGIQKYGYLPQKDNDFILATICEELGAVGGMVVVLLFLF
                     LVWRGWDVTRAAPDHFGMLLAAGLTLLIGLQAAFNVGVVTNSVPTKGISLPFVSAGGS
                     GVVFLGWAAGLLAGVGR"
     assembly_gap    5083..5182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5183..5575
                     /locus_tag="PLU99_02865"
     CDS             <5183..5575
                     /locus_tag="PLU99_02865"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOO16028.1"
                     /translation="EISHDGRPPVKIRIPDLLVIACTNPECRPEHPHDTILLDDAASW
                     QITIETSKQLGLLTPDEIRQGRERLGLSQQELQELLGLGSNSLSRWENGHVFQSRSMN
                     TLLRLVFHFPEVVSFLREKAAGQSTPAA"
     gene            5667..6593
                     /locus_tag="PLU99_02870"
     CDS             5667..6593
                     /locus_tag="PLU99_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16029.1"
                     /translation="MKLVAPGRIAVGLLSCLLIATPARSGQPVALAQVTDPSINEASG
                     IAASRLNPGCYYIHNDSDCAPCVYLIDRAGRVLLEIRLNGVEARDCEDIALAPGERPG
                     VFDVCVADIGDNQARRRNVSIYRFPEPPLPQRDKTASDPVQTPAVIEVHPARYTLRYA
                     GGPADAEAFCVHPRTGHGYILTKRIDGRSIVYKLPAPWNQNEQTLRELCTVELPMAMI
                     GLSRIVTAADISPDGRRLAVRCYVNGWEWRLPAGANDQSFDRIFTAKPIQLNLPGELQ
                     GEALCYSSDGAAFLTISEGRSPTLWETSAPAP"
     gene            6713..7855
                     /locus_tag="PLU99_02875"
     CDS             6713..7855
                     /locus_tag="PLU99_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOO16030.1"
                     /translation="MPHLPRVDSVIPLLEKMAEVQASDLHLKSGLPPVYRVGGELRRS
                     ALAPFEQGKNEVERIMQPILPQRRMSDYEERGGLDFAYHLHSGDRFRINVMKASGEMH
                     AAIRRVKGEIPNFEELHLPPIYEKLARESQEGIIIVCGVTGCGKSTTQAAMIDHINET
                     QALNIISIEDPIEYAFKPKMSIVSQREIGLDVFQFSDALRAAVRQDPDVIFVGEMRDK
                     ETMLAALQAAETGHLVFCTLHTADTMQALGRILEFFPPDQHQFIRSSLSNSIRAICAQ
                     RLLPAIDPDISRVPAVEILLGEGIVREKIREGADNDLPEIIAGSAQSGMCTFTASLAE
                     LVEKGLIYTDVAMEYAPNREQLMGLLRGIKTSAQTLVHRVKRVGTS"
     gene            8047..11103
                     /gene="fliD"
                     /locus_tag="PLU99_02880"
     CDS             8047..11103
                     /gene="fliD"
                     /locus_tag="PLU99_02880"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOO16031.1"
                     /translation="MGTISSSIGLISGIDYGTMVDQLMAIEARPRDKLTLRMSSLDAQ
                     KTALADISARLSALLARITSLTKPSFFSTSKAISSNPAVLSASTTGTAVPGSFSFTVR
                     SLARTQQLISRGYTSRNAAVAAGSLTIESAAAQVNRDTALEELNGYAGVQRGSFKITN
                     ARGESATINLREAQTVSDVLDLINTANIGVRAELRGEGIHLADTTGGTGGLRISEVGE
                     GHVAADLGFTAGLTFSADGQLTGGNLIYLSEATPLSALNDGLGLRRAVAGTDFQIQVD
                     GLDAPVKVKLSEVLTADTRLERLNHARGVELGTIRITNRAGVSEEIDLSDARTIGDVQ
                     TAIQNAISGITVVISGSGLMITDGSNEEDHDLTIEDVSGHAARDLGIAGTTDGKSIRG
                     SQILNVDTLGDVLAAINYADGNETPQGLPAVRAAIDASGRGITLTSEMGAALTLVVPE
                     ESSSQALFDLGFQPNSTGESISGGRIIGGLNTVLLKTLNGGRGLAGGIMQITANGNTV
                     QVDVTGAETLRDVIEAINTASEQSGLGIAASYHSSGNKLQIVNTADGAGSITISDVQG
                     ELAHSLGIDAGGSRITSNNLQRRYVSENTRLQDLNMGQGVSLGKIKITDSRGVSATID
                     LASERAETLQDVIDAIRGATALQLEVRINDTGDGLLITDTSDGTGKLIIEDDTGTAAR
                     DLNIAGESEGAAKQIDGSFELKIDTATADTLDQVLAQINKTRLANAAIINDGSGVAPY
                     RLSITSAASGRAGELIIDAGTTGLDLTTLTQAQDAAITLGSDDNGLLIRSTTNTITDA
                     IPGVTLTLTGTDDQPVTVTVERDLDGMLSTLKGLVDDYNGLLDRIKQYASYDSETQTK
                     GILFGESTLDAVKQRLSRALTGTISGAGGRFSRLSQLGVKVESGGRLSFDEQKFREAY
                     AADSNGVTQLFTAAKAGVAVKVKAELDQLTGTGGLLDRRSKMYTEQKEALGKRVDDLN
                     ELLELKRQKLLRSFQRMEDALAQMQSQQTSISSLGSLISSFSTTKN"
     gene            11135..11665
                     /gene="fliS"
                     /locus_tag="PLU99_02885"
     CDS             11135..11665
                     /gene="fliS"
                     /locus_tag="PLU99_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOO16032.1"
                     /translation="MPDVSKQYLRNAVLTASPEQLQLMLFDGAIRYATRGQEAMAAKD
                     FEGMFNALDRAQRIVLQLSAGLRREVNPQLVDQMSALHDFVYRRLVDANMKRDPKAID
                     DALRILRHQRETWVLLMDRLKQFVQSENGGAPESRDASNDRVASGQGAAKGPGWASGS
                     SNGERAETGSRFVAEA"
     gene            11848..13032
                     /locus_tag="PLU99_02890"
     CDS             11848..13032
                     /locus_tag="PLU99_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO16033.1"
                     /translation="MSNTCNVALIGQKFMGRAHSNAYLKASRFFNLPIQPVMHTIAGV
                     DLVSLAPFADRWGWKHYSTSWKEVVKAPDIDLVDISTPNYMHAEQAIAALAAGKHVAC
                     EKPVGATLRDAKAMKNAALRAKRSKTFVWFNYRRCPAVALAHRLVAEGQIGRIYHVRA
                     AYLQEWGGPETPLSWRYLKKYAGSGAHGDLNAHIVDMARFITGQDIVEVSGAISETFI
                     TERPLPNTGKSAHGSPSRRKRDVGKSDVDDTVLFLARFSGGAVGSFEATRLAAGHEND
                     NQIEINGSLGSLRFNFEDMNVLWYFDARESKRTAGWRRIMCTSAGNHPYAGAWWPDGH
                     IIGYEHTFVNMVADMMAVLAGEQPIVPLPDFADAFETQRVLEAAMLAAKHRAAVKLSE
                     IK"
     gene            complement(13123..>13452)
                     /locus_tag="PLU99_02895"
     CDS             complement(13123..>13452)
                     /locus_tag="PLU99_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO16034.1"
                     /translation="EYLAVPEGFVHKVEGISLDQAAMLEFLAIGAHAVRRAGLAAGER
                     ESGCTVHLVDLEYDHGPIVAQRRVPVLPGDTPDTLADRVQAAERELYPEVVQAVVERG
                     LEWLGGK"
     assembly_gap    13453..13462
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13463..13880)
                     /locus_tag="PLU99_02900"
     CDS             complement(<13463..13880)
                     /locus_tag="PLU99_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOO16035.1"
                     /translation="MATTQPTYRPLRLAVLISGAGSTLANLIERIRDGRLRGAEIVLV
                     ISSRGAVRGVEIARQAGLPVKIVRPIDFADEQAFSEAITTAVDQAEAHLVLMAGFMRY
                     WRLPPRWQGRVLNIHPALLPRFGGRGMYGGHVHAAVL"
     gene            14025..14390
                     /locus_tag="PLU99_02905"
     CDS             14025..14390
                     /locus_tag="PLU99_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16036.1"
                     /translation="MAAKTSYETQAKISVALAIVGGLSALVAAACILQKLDFQTFLFT
                     YESQGRRFLVILAGLAVGLLTGSIGFLVGFNSAGQRLNKQNRLSWTGFFLSAAVIALT
                     LCVGILLWGTRNPVHVTHA"
     gene            complement(14479..15585)
                     /locus_tag="PLU99_02910"
     CDS             complement(14479..15585)
                     /locus_tag="PLU99_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16037.1"
                     /translation="MAFTLIELLVVIALLALLIAILLPHLYEARELSKTVVCGQRLHD
                     FGIGLATYVNDNEEWLPGVNTSGVAVRTLDLLWKSEPQVLSRPDLPVQAWDWMTPLLA
                     GKTELPAVRALRFKFLLEQMRCPSQFLHSALWEGEGHYSPDRQVFKANGPYRTVSYLM
                     PAQFQFVGHAHTDETLAYKHGSDYPRWVRGRAAPDTFEYINYDYRPRLDQVGQPAGKV
                     FVADGTRYLECEGGTESLDFDAYPYVDPRYPSQGLFGSFTDPAPWWCGSTAYGVEQGS
                     LNWDGMPVDAGEYPKARGKNLALSYRHGADIGAATSNAQSNPGRMNALFFDGHVERLN
                     DRRSRDVGLWYPSGTRIQSPEEGMTRVPQDYVVP"
     gene            complement(15587..15913)
                     /locus_tag="PLU99_02915"
     CDS             complement(15587..15913)
                     /locus_tag="PLU99_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16038.1"
                     /translation="MERRKLIIAAAVSLLAVAFAVHRLKSSSPISNSVLLVCVSTGET
                     FNITRKELVCIPMTNPRTGEATLLPCHERNGVLYINQRYGSILGDLGARNRYVDPETL
                     AVRTPP"
     gene            16464..17399
                     /locus_tag="PLU99_02920"
     CDS             16464..17399
                     /locus_tag="PLU99_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16039.1"
                     /translation="MAASPVSENPTVAARSWPQRLRLIVAALLIWAGLQYLLNATLMA
                     PGLARPATLAASHHPVLAALGLAIILLVGSLVAARVAGSAYSHPGLLTAALGLALWVI
                     PAGTMTEWLMYANPQVGPPTGAAYWPLAAEYLWLLLLMVAVAGLTEIPWTALASLDRA
                     IIREEFRRTVGLHLTGRSRINGVLALLLSTLVIGVLMLILTGPATSGQTLRGQVYFAV
                     AVASACGTFAACRVLRVTEPIWYWPAPILAGLIGVLVATIRPALLLPAAYAHLNSIPA
                     WGLARPLPIEMVGVGVAVCLWALRASQRSPRTGQP"
     gene            17418..17618
                     /locus_tag="PLU99_02925"
     CDS             17418..17618
                     /locus_tag="PLU99_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16040.1"
                     /translation="MISLPPKHRAETYEEMRARLIAETSNYLTECLRHPELAVRIPTI
                     QAGRGRFPPSLAVAFWARVLSE"
     gene            17829..19202
                     /locus_tag="PLU99_02930"
     CDS             17829..19202
                     /locus_tag="PLU99_02930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO16041.1"
                     /translation="MHHHLGMRILVIQFAGAKRDRPLPRFEPQLGTLLALLKKRGHDL
                     SLVGMSRFDLETAKAALARALPQLVYADIAGICVNAARRTFEYLQQHEFLPIVVGGTY
                     PTVDPAGSLSLPGVQAAAIGEPDASLVTYLERVKDPTVGQTVLGVWMRDERGLARPDL
                     PHLVEDLNSLPFPERDLFDYANHVQHSGQIEIAVGRGCPQSCAYCINDWLEDIYDGHG
                     TWVRRRSPENVVAEINLLRERYPGVRSVKFLDHAFALDREWLTSFLQIYTQRCGLPFS
                     CHLRLNAADPEVIRQLAAAGCRQVEVELISGSDFLRNEIFDMNLNQGQIETSLQTLRA
                     AGMRTRAIVYLGAPYESEMSLAETRDLLLRCRPDEVDLRPYYPFPGTRACETSRDNGW
                     LHGRGEEQYHLHRPGIDMPACRAEHVQAFIRRLRAELRTSTGQPWWRRWSRNARVRMA
                     QLIRRGK"
     gene            complement(19287..19694)
                     /locus_tag="PLU99_02935"
     CDS             complement(19287..19694)
                     /locus_tag="PLU99_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOO16042.1"
                     /translation="MIRQITVTTRGAGLREITREIQAVVAEAGQQEGLCTVFVQHTSA
                     SLVIQENADPSARRDLEAWLNRLVREDDPLYTHTAEGPDDMPSHIKAALTATTLSIPI
                     VHGRLGLGTWQGIYLWEHRRRGSERRIIVHVGG"
     gene            complement(19723..20256)
                     /locus_tag="PLU99_02940"
     CDS             complement(19723..20256)
                     /locus_tag="PLU99_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOO16043.1"
                     /translation="MPYVWAVLLCIVNAVWFVTIIPGLPGTWLMIASTVFVALWWRGG
                     TGPGEPGMFNIWTLVAVLVIAGIAELVEFLAGVAGSKRAGGTRWGAVGALLGGLVGAV
                     VATPFIPLPLLGSLLGACIGAAAGALLLEYYTGQKLEVSIRSGVGAGVGVFFGRAAKL
                     VAGALIWLIVAVAAFWP"
     gene            complement(20360..21670)
                     /locus_tag="PLU99_02945"
     CDS             complement(20360..21670)
                     /locus_tag="PLU99_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16044.1"
                     /translation="MSAKSKVQPGALASVETARTESYQPLVRLSIMMFVEFAVWGAWS
                     VLLGKHMEHLGFSGKQIGLVYLTTALGAMLSPLVAGWIADRFMPNQIFTGIVHLLGAV
                     LLFIAWPQTQFGGMFVALLIYAILYMPTIALTNAIAFHHMKDSKKFGNIRVWGTIGWI
                     VVNWIISGYLLIWGRYQPGAWRVGDCLLFAAVLSAFMGIYCFTLPNTPPAKQAKNPYA
                     FVEAFRLMSNRNFAVLLVISFVVAIELPFYYSYTIIFLTDTNAGVGLSPGWANAAMSI
                     GQVAEIALMLLLAPSLRNLGMRMTIVLGILAWPLRYAIFAIGQPWYLVVAAQALHGIC
                     YSFFFAGGMIAVERLSPKDIRASAQGLLLFATNGVGMLIGNLISGPLYDVFVAARVSH
                     AWPKFFLVPIVVTILGAIVFAVLFKERQFQTDAAEIEQREAVAV"
     gene            21990..>22146
                     /locus_tag="PLU99_02950"
     CDS             21990..>22146
                     /locus_tag="PLU99_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO16045.1"
                     /translation="MDPQKSIALLNQAVADELQAVHQYMYWHFHLDDQGFGPLAALLK
                     RISIVEMT"
BASE COUNT         4102 a         6989 c         6956 g         3989 t
ORIGIN      
        1 cgctgaacgg ggcggtaacc gtgaacgtgc ccgacgttcg tgcggcgctg gcgcgcctgg
       61 cggttcgctg gtacggcttg gacgacggcc cggcgagtga cttacgtttg atcggcatca
      121 cgggcaccaa cggcaagacc acgaccgcca tcatgacccg cgccatcatt caggccgccg
      181 gctacaagtg cggcttgctg ggcaccgtgc gttacgactt gtgcgggcgc agcatcaccg
      241 ccaagaacac gacgccgggc gcgctggagt tggccgcgta cttgcgtgag tgcgcggacg
      301 ccggggcgcg ctatgccgtc atggaggttt ccagtcacgc cctggatcag cagcgggcgg
      361 ccgggctgca tttcgcggcg gcggggttca ccaacctgag cggcgatcac ttggactatc
      421 acggcacgtt agagaattac gctgcggcca aggcgcgctt attcgccggt ttggacgaga
      481 aggcggtggc ggtgctgaat cgcgacgatg ccagctcggc gatcatggta cgcgattgcc
      541 gcgcccggac catttggtac tcgttgcaag gcccggccga catcaccggt tccatttcgc
      601 gcaccacgat tcgcgggacg tattaccgca tgtgtgtcgg cggggcggat ctggtgctcg
      661 agaacgccgt cgtcggccgg cacaacgtgt acaacgcact gacggctgcg ggcctggcgc
      721 gggccgtggg gctgccactg gaagccatcg aagccggact gagcagcgtg cggaatatcc
      781 ccgggcgttt gcagcgcgtg ccgtgcgtga gcggggcgga cgtgttcgtc gattacgccc
      841 atactgacga tgcgctgcgc aacgtgctca gcgtgctaaa gccgctggtg caggggcagc
      901 tcatcctggt cttcggctgc ggcggggatc gcgaccgcac caagcggccg cgcatggcgc
      961 aggccgcggg tgaactggcc gaccgcatca tcgtcaccag cgacaatccg cgctcggaag
     1021 acccgcaggc gatcattgcc gaaatcatgc tcggctttgg gcagcaggtg agcgaggcgg
     1081 tggtgaccga accggatcgc gaggccgcta ttcgtttggc tttaaagacc gcgggcccgg
     1141 gcgacgtcgt gctgatcgcc ggcaagggac acgaagatta ccagattatc ggcaatcgcc
     1201 gcattcactt cgacgacgtc gaggtgacca tcagggcggc cgagcaactt gcccgcgccg
     1261 caacggagac tgcaaagaag tgataacttt gattctgagt gaggtagtcg aagccattgg
     1321 cgggcagccg gccggcgaac tgccaacctt gagcgttcgc cgcgtctcga ccgactcgcg
     1381 cagcagcgcc gagggcgacc tgttcttcgc cattcgcggc ccgcgcttcg acgggcatga
     1441 tttcatagct gatgccctac gtggcggcgc ggtggcggcc gtgatcgccg ccgatcggct
     1501 gaccgacgtg cggcagcaga ttgcgggtat cacgtcgcgc gtggtgccgc ccggggccat
     1561 catttgcgtg cctgacccgg tggtggctct tggccgcttt gcggctcacc atcggcggct
     1621 gatgtcggcg aacgttatcg cggtggtcgg aagcaacggc aaaaccacga ccaaagcgat
     1681 gattcaccac attctgtccg agcgctggaa aggacagtgt tcgcccaaga gcttcaacaa
     1741 cgaaatcggc gttccgctga ccttattgtc gtgcgagccg gccgacgagt acctcgtggt
     1801 ggaggtcgga accaatgctc ccggcgaagt ggccgcgttg gcggagctga tccaccccga
     1861 catggcggtc ctgacctgca tttccgagga gcacctcgag cgcctgggcg acctgaacgg
     1921 cgtggcggcc gaagagtgtt caatcctgca caagcttgag ccgggcggtt ttgcggcggt
     1981 caacgtggac gcgccggtgg tgcgaaggca tttgcctgac ggcggcgtca cggtcgccac
     2041 gtttggctgg aattccgaag cggacgtgcg tctgactgat gcgcgttatg aagcgccctg
     2101 gctgcatttg acgatcaacg ggcgtttcaa gtaccggctg ccgctggccg ggctgcacaa
     2161 tgcggtcaac gccgcggggg cgattacgct ggcccggcgg ctgggtatgg agcacaaaga
     2221 tattgcggcc cggctggagt cgttcgtgtc gccgccgatg cgtaccgaga tgctggagtt
     2281 cggcggcata acgctggtta acgatgcgta taacgccaac cccggcagcg cactggcggc
     2341 gctggacacg ctgtcgtcgc ttgcttgtca ggggcggcgg gtggtggtgt tcggcgagat
     2401 gcgcgagttg ggcgaacacg ccgcggagct gcaccagcag gtcggagaac atctgcgaaa
     2461 cggcaagttc gacctggtac tgctggtcgg ccgggccggc gagctgatgc cgaatctaaa
     2521 ggaggatgga cagcttttcg gtccgcgcat tttgcggtgc gcggatgtgg aggcgtgcct
     2581 ggagcggctc gcgcctgagt tgcgccgcgg cgacgtcgtt ttgctgaagg cctcgcgcgc
     2641 cgtcggtctc gaccgcctgg tcgccccact gcgcgagcgt ttggcatcgg ccacggtggc
     2701 gtgaatcaat gctgtatctg ctgctacatt ggctgacggg cggcaagggc tatgcccatg
     2761 aggacccgct tttccgcgcc accatggcga ttctgttggc gttcctgatc gtgtgggcgc
     2821 ttgggccgcg cgtcatcagc gccctgcggc gcttcaaaat cggggaccgg gcggaattcg
     2881 accacgccac gcttaatcgc atgatggcgg acaagcgcga cacgcccacg atgggtggca
     2941 ttctcattgt gatcgccatt ctcgtgtgcg tgttgctcct cgggaacctg caaagcttct
     3001 atatccggat ggggttgttc tgcctggtct ggctggccct ggtgggcggg gttgatgacg
     3061 ccttgaagct ggtatcagcg cggcgcggaa gctcgcgtga cggtctgaag ctgtgggaaa
     3121 agctgatgtt ccaggtggcg ctgggggcgc tgctggcgat attcatcctg cgggccgggc
     3181 agaccaacgc cgaggtggcg cgctttaccg acacgcaaat gccctacgaa catgcgtaca
     3241 acctcttcaa cattccgttc tggaagcaac ccattccgct gccaacgtgg attttcgtta
     3301 tcctggcggt gatcgtcctg gccggcacgt ccaacgccgt caatctcact gatggcatgg
     3361 atggtctggc gagcggctgc atggtcatga cggcgtttgc cttcatgatc ctggcgtacg
     3421 tgatcggcga ctcgaatacg gccaaatact tgttgtatcc gcccatcccg ggcgcgggcg
     3481 agttggcggt catgtgcggg gccgcggtcg gggcctgttt gggcttcctg tggtacaact
     3541 cctacccggc gcaagtcttc atgggcgaca ccggctcgct gccgctgggt gggctgatgg
     3601 ggtacgtggc tatcgtcacg cgcctggagc tgatgctctt cattgtcggc ggtatcttcg
     3661 tgatcgaggg cgcctcggta ctgatccaga ttttctacta caaattcacc ggcggcggac
     3721 ggttctttcg ctgcgccccc atacaccacc acttccatct gggcggctgg tccgaaaccc
     3781 aggtagtgat gcgcttctgg ctggtcgcgg ccttgtttgc ggccttcgcg ctggcgacta
     3841 tcaagctgag gtgaccgtgg ggactattcg gcacagcgtt atcaccgacg cgacgccggc
     3901 gccacaggaa cagttgggcc ggccgtatga cagcggcgtg ctgctattgg cctcagccct
     3961 gatgacgttc ggcgtggcga tggtttactc cgcgtcaatg acggtgcgcg gcgctcagtt
     4021 ggactggcac caatggtggc acacgccgct gcgccaatgc gttttcgcgg cgggcggctt
     4081 tctggtgatg ctgttcacag cccattgcga ctatcgttgg ctggcgtcca cgcgcaaagg
     4141 cggcctggtg cgggtgggcg ttctgtattt gatagcaatc gccacgttga tcgccgtgca
     4201 gctcgtcggg cgcgaggcgc tcggcgcgca gcgggcgctg gtcgtgctta ccagcccgtt
     4261 tacgctgagt tttcagcccg ccgagttggc caaggttgtc atggtgatct ggctggcggt
     4321 gctgctggcg cggatgcatg cccgtgcgga ggaacagcga acgcgcggtt actcggcccg
     4381 cgatcttgga atccgcaact tcacgcgcgg gtttctgccg acggtcataa gttccgggct
     4441 gctcatcggg ctgaccgggg ttgaggactt cggcacggcg gccctgatgg gcgtggtcat
     4501 ggtgatgatg ctggtgattg ccggggcgcg ctggcggcac ctcatgttgc tggcgttgat
     4561 aggcctgatg ggcggcgttg ggctgatcgc gatgaagccc tatcggctcg aacgcctcaa
     4621 gacgtacgtt tacttcctgg acgagagcaa gcccgccccc gatccgcaga aagaggggta
     4681 ccagatcaac cagtcgatgt tggccatcgg ttccggcggc tggttcggcc gcggcctggg
     4741 tgcgggcatt cagaagtacg gctatctgcc gcagaaagac aacgatttca ttctggcgac
     4801 catctgcgag gaactgggcg cggtcggcgg aatggtggtg gtgttgctgt ttctgttttt
     4861 agtatggcgc ggctgggacg tgacgcgcgc cgcgcccgat catttcggga tgctcctggc
     4921 ggcggggctg acgctgctga tcggtctgca agccgcgttc aacgtcggcg tggtgacgaa
     4981 cagcgtgccg accaagggca tatcgctgcc gtttgtgtcg gcgggcggct cgggcgtggt
     5041 ttttctgggc tgggcggcgg gtttgctggc gggggtggga cgnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nngaaatcag ccacgacggc cggccgccgg tgaagatacg
     5221 cattcccgat ctgctggtta ttgcctgcac caatccagaa tgccgcccag agcacccgca
     5281 cgatacgatt ttgctggacg atgcggccag ttggcaaatc acaatcgaga cgtccaaaca
     5341 actgggtttg cttacgcccg acgaaatccg gcaagggcgc gagcggctgg gcttgagtca
     5401 gcaagaactg caggagctac tggggctggg cagcaattcg ctgtcgcgct gggaaaacgg
     5461 acacgtgttc caatcgcgaa gtatgaacac gctgctgcgg ctggtgttcc atttcccgga
     5521 ggtcgtgtcg tttctgcgcg aaaaagccgc gggccaatct acgccggccg cctgaggcac
     5581 acggcactaa tcagaagcat aaaggtgctc gcctgattag acgcgatgag gctatcttga
     5641 ggtcgtttct cgcggcaagg tgcgttatga agctcgttgc gcctgggcgg attgccgttg
     5701 gcctgctgag ctgccttctg attgccactc ccgcgcgatc tgggcagccc gtcgcgcttg
     5761 cccaagtcac tgacccctcc atcaatgaag cctccggcat cgctgccagc cggctcaatc
     5821 caggctgcta ttacattcat aatgactcag actgcgcccc gtgcgtgtat ttgattgacc
     5881 gtgccggccg cgtactcctt gaaatccggc tcaatggcgt ggaggcgcgt gactgcgagg
     5941 acatcgcgct agcgcccggc gagcgcccgg gcgtcttcga cgtgtgcgtc gcggacatcg
     6001 gcgacaacca ggcccggcgc aggaacgtca gcatctaccg tttcccggaa ccgcctctgc
     6061 cgcagcgcga taaaaccgcg agtgatccgg tccagacgcc cgccgtcatc gaggtccatc
     6121 cggcccgcta caccctccgc tacgccggcg gcccggccga cgccgaggcc ttctgcgttc
     6181 acccacgaac cggccatggc tacatcctta cgaaacgtat cgatggccgc agcatcgttt
     6241 acaagctgcc cgcgccgtgg aatcagaacg aacaaaccct tcgggaattg tgcacggtgg
     6301 agctgccgat ggccatgatc ggcttgagcc gcatcgttac ggccgccgac atctcacccg
     6361 atggtcgccg cctggcagtc cgctgctacg tcaacggctg ggagtggcgc ttgccggcgg
     6421 gcgcgaatga ccagagcttc gatcgaatct tcacggccaa gcccatccag ttgaacctgc
     6481 ccggcgaact ccaaggcgag gccctgtgct attcgtccga cggcgccgcg tttctcacga
     6541 tcagcgaggg ccggtcgcct accctctggg agacttcggc gccggcccca taaccgtaag
     6601 cggcctcaca acggattgca ggcacgcgct aacttactct tgccgcggac ggccggccgc
     6661 acgtatcatg cggcatctgg cattcacctg tccctcgcgt ttgaggtgtg ctatgccgca
     6721 tttgccgcgc gtcgattccg tgatcccgct gttggagaaa atggccgaag tgcaggccag
     6781 cgacctgcac ctcaagagtg gtctgccgcc ggtttaccgc gttggcggtg agctgcgccg
     6841 ctcggccctg gccccgttcg agcagggcaa gaacgaggtc gaacgcatca tgcagcccat
     6901 cctgccgcag cgccgcatga gcgactatga agagcggggc gggctggatt tcgcctacca
     6961 tttgcacagc ggcgaccgtt tccgcatcaa tgtcatgaag gcctcgggcg agatgcacgc
     7021 cgccattcgc cgcgtgaagg gcgagatccc gaacttcgag gaactgcacc tgccccccat
     7081 ttacgaaaag ctcgcccgcg agtcgcagga gggcattatc atcgtttgtg gcgttacggg
     7141 gtgcggcaaa agcacgaccc aggccgcgat gatcgaccac atcaacgaga cccaggcgct
     7201 gaacatcatc agcatcgagg atccgataga atacgcgttc aagccgaaaa tgagcatcgt
     7261 cagccagcgt gaaatcggct tggacgtgtt ccaattctca gatgcgttgc gtgccgccgt
     7321 ccgccaagac ccggacgtca tcttcgtggg cgagatgcgc gacaaggaaa ccatgctcgc
     7381 cgccctgcaa gccgccgaaa ccggccacct ggtcttctgc acgctgcaca cggccgacac
     7441 catgcaggcc ctcgggcgta ttctagaatt cttcccgccg gaccagcacc aattcatccg
     7501 cagctcgttg tccaacagca tccgcgccat ctgtgcccaa cgtcttttgc cggcaattga
     7561 cccggacatc agccgagtcc cggccgtcga aattctgttg ggcgaaggca tcgtgcgcga
     7621 gaagattcgc gaaggcgcgg ataacgattt gccggaaatc atcgccggca gcgcgcagtc
     7681 cggcatgtgc accttcaccg cctcgctcgc cgagctggtg gaaaagggcc ttatctatac
     7741 ggacgtcgcg atggaatacg cccccaaccg cgagcaactc atgggcttgc tgcggggcat
     7801 caagacctcg gcccaaacgc tggtgcaccg cgtcaaacgc gttgggacca gctagctggc
     7861 gtacctgagc acagatcagc ccgcgagtcc gtaaccctcg cccccctgat tcccagtcct
     7921 ttctgatccc gttatgggac gactaattgc gccgcggtat ccaccttgca ctcaaccagg
     7981 ccgatagcgc tggtggcggt gcgccatcgg ccacgccact catggcttta ccccaggagg
     8041 ttcgtggtgg gaactataag ttccagcatc gggctcattt cgggcatcga ttatggcacg
     8101 atggtcgacc agttgatggc gatcgaggcg cggccacgcg ataaactcac gctccgcatg
     8161 agcagtctcg acgcgcaaaa aacggccttg gcggacattt ctgcgcgact gtcggctctg
     8221 ctcgcccgta ttacttcgct gaccaaacca agttttttct ctacttccaa agccatctct
     8281 tcgaacccgg cggtgctttc ggcttccacg accggcacgg ccgttcccgg ctccttctcg
     8341 ttcaccgtcc gctcgctggc gcggacgcag cagctcatca gccgcggata taccagccgt
     8401 aacgccgccg tggcggccgg ttcgctgacg atcgagtctg ccgcggcgca ggtcaatcgt
     8461 gacaccgcgc tggaggagtt gaacggctac gcgggagtgc agcgcggctc gttcaagatc
     8521 acgaatgctc ggggagagag tgcgacaatc aacctgcgag aggcccagac ggtttccgac
     8581 gtactggatt tgatcaacac tgcgaacatc ggcgtacgcg ccgagctgcg cggggagggc
     8641 attcacctcg ccgacaccac cggcggcacg ggcggactgc ggatcagcga agtgggcgag
     8701 ggccatgtcg cggctgacct ggggtttacg gcgggcctga cattctcggc cgacggccag
     8761 ttgacgggcg gcaacctgat ctacctctca gaggccacgc cgctgagcgc gctgaacgat
     8821 ggcctcggtc tgcggcgggc ggtggcgggg acggattttc aaatccaggt ggatggactc
     8881 gacgcgccgg tgaaggtgaa actgagcgag gttttaaccg cggacacgcg tttggaacgg
     8941 ctgaatcacg cgcgcggcgt cgaattggga acgattcgca tcaccaaccg cgctggggtg
     9001 tccgaggaaa tcgatctgtc agacgcccgc accatcggcg acgtgcaaac cgccattcag
     9061 aacgccatca gcggcattac ggtggtgatc tccggcagcg ggttgatgat cactgacggc
     9121 agcaatgaag aagatcatga cttgaccatt gaggacgtgt caggtcacgc ggcacgcgac
     9181 ctgggaatcg ccggcacaac ggacggcaag agcatccgcg gctcgcaaat tctgaacgtt
     9241 gatacgctgg gtgacgtgct ggccgccatc aattacgcag acggaaacga gacgccccag
     9301 ggcctgccgg cagtgcgggc ggcaatcgac gcgtccggcc gcggcatcac attaaccagc
     9361 gaaatgggcg cggccctgac gctggttgtg ccggaggaga gctcgtctca ggccctgttc
     9421 gatctgggct ttcagccgaa ctccaccggg gagagcatca gcggcgggcg gatcatcggg
     9481 gggctcaaca cagttctgct gaagacgctt aatggcgggc gcggcctggc gggcggaatc
     9541 atgcagatca ccgccaacgg gaacacggtt caagtggacg tgaccggcgc ggagaccttg
     9601 cgcgacgtaa tcgaggcgat caacacggcg tccgagcaga gcggtctggg aatcgcggcc
     9661 agctatcact caagtggaaa caagctgcag atcgtcaaca ccgccgacgg ggccgggtcg
     9721 atcacgatca gcgacgtgca gggcgagctg gcccacagcc tgggtatcga cgcgggcggc
     9781 agccgcatca cgagcaacaa cctgcaacgg cggtacgtat ccgagaacac gcgtttgcag
     9841 gatttgaata tggggcaggg cgtttcgctc ggcaagatca agattacaga ctcgcgcggc
     9901 gtctcagcga ccatcgattt ggcctcggaa cgggccgaga cgctgcagga cgtaatcgac
     9961 gccatccgcg gagcgaccgc cctgcaactc gaggtgcgca ttaacgatac cggcgacggc
    10021 ctactcatta cggacacttc cgacggtacc ggcaagctga taatcgagga cgataccggc
    10081 acggcggcgc gcgatctgaa catcgccgga gaatcggaag gcgccgccaa gcagatcgac
    10141 ggttcgttcg aacttaagat cgatacagcc accgccgaca cgttggacca ggtactggcg
    10201 cagatcaaca agacgcgcct ggccaacgcc gccattatca acgacggctc gggcgtggcc
    10261 ccgtaccggc taagcattac gtccgccgcc agcgggcggg ccggtgagtt aattatcgat
    10321 gcgggaacca ccgggctgga cctgacgaca ctgacgcagg cccaggatgc cgcaatcacg
    10381 ctcggcagtg acgacaatgg cctgctgatt cgcagtacaa ccaacacgat tactgacgcc
    10441 atacccggag tgacgctcac actgaccggt acagatgatc aacccgtcac agtcacagta
    10501 gagcgagatt tggacggcat gctgtcaacg ctgaagggcc tggtcgatga ctacaacggc
    10561 ctgctcgacc gcataaagca gtatgccagt tatgattctg aaacgcagac gaagggcata
    10621 ctcttcggcg agagcacgct ggacgcggta aagcagcggc taagccgggc gctcaccggc
    10681 actatttcgg gggccggcgg acgcttttcg cgcctgtcgc agctaggcgt caaggtcgaa
    10741 tcaggcgggc ggctcagctt tgatgagcag aagttccgcg aggcttacgc ggcggattcg
    10801 aacggtgtaa cgcaactctt cacggccgcc aaggccggcg tggccgtcaa ggtcaaggcg
    10861 gagctggatc agttgaccgg gacgggcggg ctgctggatc gtcgcagcaa gatgtatact
    10921 gagcagaaag aggcccttgg caagcgggtg gacgatttga acgagctact cgagctcaaa
    10981 cggcagaagc ttctgcggag ctttcagcgg atggaagacg cgctggcaca aatgcagtcc
    11041 caacaaacgt ccatcagcag cctcggaagc ttgatcagtt cattcagtac caccaagaac
    11101 taaaccgcga ctgcggccga ccgatactgc caatatgcca gatgtctcga agcaatatct
    11161 ccggaacgcc gtgctgaccg cctcgccgga gcagttgcag ctcatgctgt tcgacggcgc
    11221 gatccgttac gcgacgcgcg gccaggaggc gatggccgcg aaagatttcg aggggatgtt
    11281 caacgcgctg gaccgcgcgc agcgcatcgt gttgcagctc tcggccggtc tgcggcggga
    11341 agtcaatccg caacttgtag accagatgtc ggcgctgcac gacttcgtct accggcggct
    11401 ggtcgacgcg aacatgaagc gcgatccgaa agccatcgac gatgccttac gcatacttcg
    11461 ccatcagcgg gaaacgtggg ttttgctgat ggacagactc aagcagttcg tccaatcaga
    11521 aaacgggggc gcgccggaaa gccgggacgc gtcaaatgac cgggtcgcgt caggccaggg
    11581 ggcggcaaag ggtccgggtt gggcatccgg ctcgtcaaat ggcgagcgtg cagaaaccgg
    11641 gtcccgcttc gtcgccgagg cgtgagcagc ggcaacgtct cgagcctgac acacggcact
    11701 aatgacgctc aggcctccag tggcccgtgg cccacggccg cgcagcacat cgccagattg
    11761 ttccccgacg ccctcgacag accggagccg gatttgtaat attccgcccg tacgtgcggc
    11821 gcggttctcg ggttctgagg gtgacgcatg tccaacactt gcaacgttgc gttgatcggc
    11881 cagaagttca tggggcgggc tcacagcaat gcctatttaa aggcttctcg gttctttaat
    11941 ctgccgattc agcccgtgat gcacacgatc gcgggcgtcg atctggtttc gctcgcccca
    12001 tttgccgaca gatggggttg gaaacattac agcacgtcct ggaaggaggt cgtgaaggct
    12061 cccgatatcg acctcgtcga tatcagtact cccaattaca tgcatgccga gcaggccata
    12121 gcggcgctcg cggccggcaa gcacgtggcc tgcgaaaaac cggttggcgc gacgctgcgc
    12181 gatgctaaag ccatgaagaa cgcggccttg cgagccaaac gatccaaaac gttcgtttgg
    12241 ttcaactacc gccgctgccc ggccgtggct ttggcgcaca ggttggtcgc agaggggcaa
    12301 atcgggcgaa tctaccatgt ccgtgccgcc tacctgcagg agtggggcgg accggagacg
    12361 ccgctgagct ggcgctacct gaagaagtac gccggctccg gggcccatgg cgacctgaac
    12421 gcgcatatcg ttgatatggc aaggtttata accggccagg acatcgtcga ggtcagcggg
    12481 gcgatctcag agacgttcat taccgagcgg ccgctgccga acaccggcaa atccgcccat
    12541 ggaagtccta gtcgccgaaa acgcgacgtg ggcaagagcg acgtcgacga cacggtcttg
    12601 ttcctggcac gcttcagcgg cggggcggtc ggcagcttcg aggcgacccg cctggccgcc
    12661 ggtcatgaaa acgacaatca gattgaaatt aacggctcgc tcgggtcgct gcgcttcaat
    12721 tttgaagaca tgaacgtgct gtggtatttc gacgcgcgcg aaagcaagcg cacggccggc
    12781 tggcggcgga tcatgtgtac gtcggccggc aatcacccct atgccggggc gtggtggccg
    12841 gacgggcaca tcatcggcta cgagcacacg ttcgtgaaca tggtggcgga catgatggcc
    12901 gtgctggcag gcgagcagcc gattgtcccg ctgccggatt tcgccgatgc attcgagacg
    12961 cagcgcgtgc ttgaagcggc gatgctggcg gccaagcacc gcgcggcggt gaagctgtct
    13021 gaaatcaagt aaggtggggt atgatcgcgg cgccggggga ttagtgccct cgctcgcgct
    13081 tcgggctcgg atcgggcgcg cttggatcag gggggattac ctttacttcc ctccaagcca
    13141 ctccagcccg cgctccacaa ccgcctgcac cacctccgga tagagttcgc gctcggcggc
    13201 ctgcacgcga tccgcgagcg tgtcgggcgt atcgccgggc agcaccggca cgcggcgctg
    13261 cgcaacgatc gggccgtgat catattccag gtccaccaag tgcaccgtgc agccgctctc
    13321 gcgctcgccc gccgccaggc ccgcacgccg caccgcgtgg gcgccgatgg cgaggaattc
    13381 gagcatcgcg gcctggtcga ggctgatgcc ctcgaccttg tgcacaaagc cttcgggcac
    13441 ggccaggtac tcnnnnnnnn nnccagcacc gcggcgtgta cgtggccgcc atacataccc
    13501 cggccgccga atcgaggcag cagggcgggg tgaatattca agacgcgacc ctgccagcgc
    13561 ggcggaaggc gccaatacct catgaagccc gccatgagga ccaggtgtgc ttcggcctgg
    13621 tccacagcgg tcgtaatcgc ttccgaaaat gcttgctcgt cggcgaagtc gattgggcgg
    13681 acgattttga ccggcagacc cgcctggcgg gcaatctcga ccccgcgaac cgcgccgcgc
    13741 gagctgatta ccagtacgat ctcggcgccg cgcagccggc cgtccctgat ccgctcgatc
    13801 aggttcgcca gcgtagagcc tgccccggaa attaacaccg ccagccgaag cggccgataa
    13861 gttggttgag tggtcgccat gcccgtcatc ttaccggttg ggtgggccgc gttgatagtg
    13921 cccactgcgg tcagttataa taccggcctg accggccccg taccgggccg cttcagggcg
    13981 gaaagaggcg ctggaatcga ccgcgcgacc gagaggatgt aaacgtggct gcaaaaacca
    14041 gctatgaaac gcaggccaag atcagcgtcg ccctggcgat cgtcggcggg ctctcggccc
    14101 tggtggccgc cgcctgcatt ctgcaaaaac tcgacttcca gacgtttctc ttcacgtatg
    14161 agtcgcaggg ccggcggttc ctggtgatat tggcgggttt ggcggtcggg ctactgaccg
    14221 gcagcatcgg cttcctggtc ggcttcaaca gcgccgggca gcgcctcaac aagcagaacc
    14281 gcctctcctg gaccgggttc tttctcagcg ccgcagtcat cgccctgacg ctttgcgtcg
    14341 gcattctgct atggggtacg cgcaacccgg tgcatgtgac gcacgcttag gtcacgagct
    14401 gagtcccgca gcgtccgtag gtcgttatca atctttgtta caaccggccg cgaggaaatt
    14461 gtcgatcgct ggctcgcact agggcacgac atagtcctgc ggcacgcgcg tcatgccttc
    14521 ttcgggtgac tggatccggg tgccgctcgg gtaccacagc ccgacatcgc gtgaacggcg
    14581 atcgtttagc cgttctacgt gtccatcgaa gaacagagcg ttcattcgcc ccggattgct
    14641 ctgtgcgttg ctggtggccg ccccgatgtc tgccccgtgg cggtaactta gggccaagtt
    14701 ctttccgcgg gccttgggat actcgcccgc gtccaccggc atcccgtccc aattgagcga
    14761 gccctgctca acgccgtaag cggtcgaacc gcaccaccac ggcgcggggt ctgtgaaact
    14821 gccgaacagg ccttgcgagg gatagcgtgg atcgacgtac gggtaagcgt cgaaatccag
    14881 actctcggtg ccaccctcgc actcaaggta gcgcgtgcca tcggcgacga agaccttgcc
    14941 ggccggctgg ccgacctggt cgagccgcgg gcggtaatcg tagttgatgt actcaaaagt
    15001 gtcgggcgcg gcccgcccgc gcacccagcg cgggtaatcg gagccatgtt tgtacgccag
    15061 cgtttcgtcc gtgtgggcat ggccaacgaa ttggaactgt gccggcatca ggtagctgac
    15121 cgtgcggtag gggccgtttg ccttgaatac ttgccggtcg ggcgagtaat gcccctctcc
    15181 ctcccatagc gccgagtgca ggaactgact cgggcaccgc atctgctcca gcaggaactt
    15241 gaaccgcagc gcccgtacgg cgggcagctc ggtcttgccg gccaggaggg gcgtcatcca
    15301 atcccacgcc tgcaccggca ggtcggggcg gctgagcacc tgtggctcgc tcttccacaa
    15361 gaggtcgaga gtgcggacgg ccacgcccga ggtgttgact cccggcagcc actcctcgtt
    15421 gtcgttcacg taagtggcca gaccaatgcc gaagtcgtgc aggcgctgac cacatacgac
    15481 tgtcttgctc agctcgcgcg cctcgtacag atgaggcagc agtatggcga tcagcaatgc
    15541 cagcagagca atgactacca acagttcgat caatgtgaat gccatattac ggcggcgtgc
    15601 gcaccgccag cgtttcggga tccacatacc ggttcctcgc tcccagatcc cctagaattg
    15661 atccataacg ctggtttatg tacagcactc cgttgcgttc gtggcacggc aggagcgtcg
    15721 cctctcctgt ccgcgggttt gtcatgggta tgcagaccag ttctttgcgg gttatgttga
    15781 aggtctcgcc ggttgacacg caaacgagaa gcaccgaatt ggaaatcgga ctggatgact
    15841 tgagccggtg gaccgcaaac gcaactgcca gtagagacac tgccgccgca atgatcagtt
    15901 tcctgcgttc cacggccagg cccgcgtgaa ctgcgccact ggtccggcgc cagcgctagc
    15961 ccagaagagg ccgcatcaga cgccgataga tacgattgca ttgatctaga cagcgcacat
    16021 cctaccggta aatgctcgta atgtgaactt cccgttacag atttgcgcaa aagcgcaaat
    16081 gtgccgattg cgaaaaaaaa gatcgcaaac gtgctgcttg tccacgggta accgccaagg
    16141 cgctattcgg acaaacgccg acgcggtgcg acgcgaaacc ggttccgcgg gcagcctcat
    16201 tccaaaatgc gccatgcact gtcgcctgct gccggcgcgc cagccgcttg cgcaattcgg
    16261 gcgggaggcg gacgccgatc agcgcacaag tcaccgccag ccgctgccac ccaccctaaa
    16321 gcgaccccgc caggcggtcc aagccatgtt cgcgattgac ccgttccacg cctacgccta
    16381 gcctataagc tgcggctagt ccggccggct gaaattcccc ggcaggcagg ctgttacagc
    16441 aagactccga aatggtttgc gaaatggctg cttcacccgt ttctgagaat ccaaccgtcg
    16501 cggcacgctc ctggccgcag cgcctgcggc tgatcgtggc cgcgcttcta atctgggccg
    16561 ggctgcaata ccttttgaac gcgacgctga tggcgcccgg tctggcccgc ccggcgaccc
    16621 tggccgccag ccatcatccg gtgctggccg cgctcgggct cgcaattatc ctgctcgtcg
    16681 gtagcttggt ggcggcgcgc gtggctggct cggcgtattc gcaccccggg ctgctgaccg
    16741 cggcgctggg gctggccctg tgggtcatcc cggccggcac catgaccgag tggctgatgt
    16801 acgcgaatcc gcaggtcgga ccgcccaccg gtgcggccta ctggcccctg gctgctgaat
    16861 acctgtggct gctgctcctg atggtggccg tggcgggtct gaccgagatc ccctggaccg
    16921 cactggcaag ccttgatcgc gcaatcatcc gcgaggaatt ccgccggacc gtcggcctgc
    16981 acctcacggg ccgttcgcgc atcaatgggg tactcgcgct gctgctttcc acgcttgtca
    17041 ttggcgtgct gatgctgatc ctgaccgggc cggcgacttc gggtcagacc ttgcggggcc
    17101 aggtgtactt cgccgtcgcc gtggccagcg cttgcggcac gttcgccgcc tgccgcgtgc
    17161 ttcgcgtaac cgaaccaatc tggtactggc cggcgccgat cctggccggg ctgatagggg
    17221 ttttggtggc cacgatacgt cccgcgctat tgctgccggc cgcctacgcg cacttgaatt
    17281 cgatccccgc ctggggtctg gccagaccgc tgccgatcga aatggtgggc gtgggcgtgg
    17341 cggtttgcct gtgggcgctg cgcgccagtc agcgctcccc gcgcaccggg cagccgtgag
    17401 catgagcagg gccagccatg atctcgcttc cgccgaagca ccgtgccgaa acctacgagg
    17461 agatgcgggc gcggctcatc gctgaaacgt ccaactactt gaccgaatgc ctgcggcacc
    17521 cagagctggc cgtccgcatc cccaccatcc aggccggccg gggccgattt ccgccgtcgc
    17581 tggcggtcgc attctgggcg cgagtactga gcgagtgatt gcgcctgacg ccgttaacga
    17641 ggggcatggt gggtgccatg cccaagcccg cgcagcgggc gcgggcatgc cggcgccagt
    17701 tgctccgcac ctgccgggcc gttaacgagg ggcatgccca cgcttagagc tggggcatgg
    17761 cacccgccca gggaccatga ccacgggcgg gctggccgaa cgggcggctt gcccgaagcc
    17821 gcttgtgcgt gcatcatcac cttgggatgc gcatcctcgt aattcaattc gccggtgcta
    17881 aacgcgaccg gccgctgccg cgctttgaac ctcaactcgg cacgctcctg gcgctgctga
    17941 aaaaacgggg gcacgacctg tcattggtcg gcatgtcgcg cttcgacctg gagaccgcca
    18001 aggctgccct tgcccgcgcc ctgccgcagc tcgtttacgc cgacatcgcc ggcatttgcg
    18061 tgaacgccgc acgccgcacc ttcgagtacc tacagcagca tgagttcctg cccatcgtcg
    18121 tcggcggcac atacccgact gtcgatccgg ccggcagcct cagcctgccc ggcgtccagg
    18181 ccgccgccat cggggaaccc gacgcctcgc tcgtcaccta ccttgagcgc gtcaaggacc
    18241 cgacggttgg ccagaccgta ctcggcgtct ggatgcgcga cgagcgcggc ctggcccgcc
    18301 cggacttgcc gcacctggtc gaagacctca attcgctccc gttcccggag cgcgacctgt
    18361 tcgactacgc caaccacgtc caacactccg gccagatcga gatcgcggtt ggccgcggtt
    18421 gtccacaaag ctgcgcctac tgcatcaacg actggctcga agatatctac gacgggcacg
    18481 gcacgtgggt gcgccgccgc tcaccggaga acgtggtcgc ggaaatcaac ctgctgcgcg
    18541 agcgctatcc gggcgtgcgg agcgtgaagt ttttggatca cgccttcgcc ctcgaccgcg
    18601 aatggctgac cagcttcctg caaatctaca ctcagcgctg cggcctgccg ttctcctgcc
    18661 acctgcgcct caacgccgct gacccggaag tgatccgaca gcttgcggcc gccggctgcc
    18721 ggcaagtaga ggtcgagtta atcagcggca gcgatttcct gcgcaacgaa attttcgata
    18781 tgaacttgaa ccagggccag atcgaaacgt cgctgcaaac gctccgtgcc gcgggcatga
    18841 ggacgcgcgc cattgtctac ctgggcgccc cgtatgaaag cgagatgtcg ctggccgaga
    18901 cgcgcgacct cctgctgcgc tgccgccccg acgaggtcga cctccgtccc tactatcctt
    18961 tccctggaac gcgcgcttgc gaaaccagcc gcgacaacgg ctggctacac ggccgcggcg
    19021 aggaacagta ccacctgcac cggccgggaa tcgacatgcc ggcgtgccgc gccgaacacg
    19081 tacaggcctt catccgccgc ctgcgagccg agctgcgcac cagcaccggc caaccctggt
    19141 ggcgccgctg gtcgcgtaat gcccgcgtcc gcatggccca actaattaga cgcggcaaat
    19201 agtcaagccg ctttcgcatc ttcccgcccc ccgcgcgcgc cgttatcacc ctatcacgtt
    19261 atcactttat cactgacggc cgctggctac cctccgacat gcacgatgat ccgccgctcc
    19321 gacccgcgcc gccgatgctc ccacaagtag atcccttgcc acgttccgag cccgagccgc
    19381 ccgtgcacaa ttggaatcga tagcgtcgtt gccgtcagcg cggccttaat gtgcgacggc
    19441 atgtcgtccg gcccctcggc agtgtgcgtg tacagtgggt catcctcccg caccagccgg
    19501 ttcagccacg cctccaaatc gcgccgcgcc gatggatccg cgttctcctg aataaccagg
    19561 ctggccgaag tgtgctgcac gaaaacggtg cacaaaccct cctgctgtcc ggcttcggcc
    19621 acaaccgcct gaatttcgcg ggtgatctcc cgcagccccg ccccacgcgt cgttacggta
    19681 atctgtcgaa tcatggtccg cgatgatacg ccggtaatcg cgctacggcc aaaacgcggc
    19741 gacggccacg atcagccaga tgagcgctcc ggccaccaac ttcgccgcac ggccaaagaa
    19801 aactccgacg ccggctccga cgccggagcg aatcgaaacc tccagctttt gcccggtgta
    19861 gtactccagc agcagcgccc ccgccgccgc cccgatgcac gcccccagca gcgagcccaa
    19921 cagcggcagc ggaatgaagg gcgttgcaac aacggcccca accagcccgc ccagcagcgc
    19981 gcctaccgcc ccccagcgcg tccccccggc tcgttttgaa cccgccacgc cggccaggaa
    20041 ctcgaccaac tccgcaatgc ccgcaattac caagaccgcc accaacgtcc agatgttgaa
    20101 catcccgggt tccccggggc ccgttccgcc gcgccaccac aaggcaacga aaaccgttga
    20161 ggcgatcatc agccacgtcc cgggcaggcc cggaataatg gtgacaaacc agactgcatt
    20221 cacgatgcac aggagcactg cccagacata aggcatgcac aacctcgtga agcaccggtt
    20281 ttagccggtc agatccagca ggccggttca ttgaccggca aaggagcgcc cccacaaaag
    20341 ccgctcgcac gaaaatactt tagacggcca cggcttcgcg ctgctcgatt tccgccgcat
    20401 ccgtttggaa ttgccgttct ttgaacagca cggcgaacac aattgcgccg agaatcgtga
    20461 ccacgatcgg caccaaaaag aacttcggcc aggcgtggct cacgcgcgct gcgacgaaca
    20521 cgtcgtacag cgggccgctg atcaggttgc cgatcagcat gcccacgcca ttcgtcgcga
    20581 acaacagcaa gccctgggcg ctggcacgaa tgtcttttgg cgacagccgt tccaccgcga
    20641 tcatgccgcc ggcgaaaaag aatgagtagc agattccgtg cagggcctgg gcggccacga
    20701 ccaggtacca cggctggcca atcgcgaata tcgcataacg cagcggccag gcgagaattc
    20761 ccagcacgat cgtcatccgc atgcccaggt tacgcaaaga cggcgccagc agcagcatca
    20821 gcgcaatctc ggccacctgt ccgatactca tggcggcgtt cgcccagccc ggcgaaaggc
    20881 cgaccccggc gttcgtgtcg gtcaggaaaa tgatcgtgta gctgtagtag aagggaagct
    20941 cgatcgccac cacaaaggag atcaccagga gcaccgcgaa attgcgattc gacataaggc
    21001 ggaaggcctc gacgaatgcg tagggatttt tggcctgttt agcgggcggc gtgtttggca
    21061 gggtgaagca ataaatcccc atgaatgcag acagcaccgc cgcgaacaac aggcaatcgc
    21121 ccacacgcca ggcgcccggc tggtaccgcc cccaaatgag caggtagccg ctgatgatcc
    21181 agttcaccac gatccagccg atcgtgcccc acacccggat gttcccgaat ttcttggagt
    21241 ccttcatgtg atggaaggcg atcgcgttcg tcagggcgat ggtgggcata tacaagattg
    21301 catagatcag cagtgccacg aacatgccgc cgaattgcgt ttgcggccag gcgataaaga
    21361 gcagcaccgc gccgagcaag tgcacgatcc cggtgaaaat ctggttgggc atgaagcgat
    21421 ccgcgatcca gcccgcgacc agcggcgaga gcattgctcc gagcgctgtc gtaagataca
    21481 caagcccaat ctgcttgccg ctgaagccca gatgctccat gtgcttgccg agcaatactg
    21541 accacgcgcc ccacacggcg aactccacga acatcatgat cgagagccgg accagtggct
    21601 ggtacgactc cgtgcgcgcg gtttccaccg atgccagcgc gcccggctgg actttcgact
    21661 tggcagacat caaacgggct cctttcgtaa ttcctttcag ctcggcatct tagcgacacc
    21721 tggcggcgcc gccagcgccg caaatcagcg ctcggcacgg caatgtggca tcgatgccgc
    21781 cgaacgcgcc ttatcgggcg gccctgatta cgcccggcgg caggtcgcat gcgaggtcaa
    21841 gcgtgtcgcc cccttgatcc ataggtgctt agatccctgg gttgactcca ctcccgtcca
    21901 gttgtagcgt gacgtgcccc ggtcgtcccg ctctggcagg gcgcgctgca cggtgcgacc
    21961 ggattgggcc gaaccagacg gaggatatga tggacccaca aaagagcatc gccctgttga
    22021 atcaagcagt cgccgacgag ctgcaggctg tgcaccaata catgtattgg cacttccacc
    22081 tggacgacca gggtttcggc ccgttggctg ccctgctgaa gcgcatctcg atcgtcgaga
    22141 tgacgc
//
LOCUS       DAOSYS010000027        22131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1930_length_22131_cov_5.245017, whole genome shotgun sequence.
ACCESSION   DAOSYS010000027 DAOSYS010000000
VERSION     DAOSYS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 22131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22131
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1930_length_22131_cov_5.245017"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="pruA"
                     /locus_tag="PLU99_02955"
     CDS             complement(<1..1493)
                     /gene="pruA"
                     /locus_tag="PLU99_02955"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOO16046.1"
                     /translation="MLVPYRPEPYVNFTEPGPRQQMLAALQQVRQQLGRTYPLRIGGK
                     KIETEKKIASVMPARHETVVGHVSKAGREQAAQAMEAALKTFPTWSRVDPETRARILF
                     KAAAIMRRRVYEVAAWMCFEVSKSWIEAYADACEAIDFLEFYGREMVRLGGPQPVTPY
                     PGEDNELLYMPLGVGVVIPPWNFPLAICTGMTSAAIVTGNTVVLKPASTSPVIAAKMV
                     EIFEEAGLPPGVLNFCPGSGGEIGDYLVEHPKTRFVSFTGSMEVGLNIFQKCGMVGKD
                     QIWLKRSVLEMGGKDCILVDETADVEAAAEGIVASAFGFQGQKCSACSRAILHQDVYD
                     AVLERVVERAKKLTIGDTTSEENLYMGAVIDDASFKKINDYIAIGKKEGRQVLGGTSV
                     PAGGYFVPPTIIADVDRNARIAQEEIFGPVLAVIKAKDFDDGLAIVNGTRFGLTGALY
                     SNDRFRLERARHEFHVGNLYFNRKCTGALVDVQPFGGFNMSGTDSKAG"
     gene            1824..2612
                     /locus_tag="PLU99_02960"
     CDS             1824..2612
                     /locus_tag="PLU99_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HOO16047.1"
                     /translation="MAAQPVFKLDLSERFPHLPRAPIVEAVIHWRARSEQRLAREDLR
                     RELTSALPDYPTIKTQHELHFESALGPDGAAVSSRDEWHGFRLESTDGRYIAQFTRGG
                     FVFSRVTPYENWDRFAAEAKRLWQIHVSLAKPSEIERLGVRFINRIVPVDLSRLDDIL
                     VLPPQTPPGLPLPIDEFLQRSLFDVPGHPYRVNVIQTTQPPIPPETDTLGLILDIDVF
                     TTASIPLNDALADTRLAEMRWLKNKVFFSLISPQATARFQEDDA"
     gene            2609..3067
                     /locus_tag="PLU99_02965"
     CDS             2609..3067
                     /locus_tag="PLU99_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16048.1"
                     /translation="MRLAISGYGVSSAARYIDATTRRNRRHLHQSPAFGIESVLRDDL
                     AAAWAECRQPGWDGYDALPVAQDALRNMYIFLEALPLGSPHPTIAADPHGYFSAEWYR
                     GPRRVLSVAVTDDDLLHYAALVGPNRVCGTETFYGEIPEAILSLMRRVYS"
     gene            3120..3545
                     /locus_tag="PLU99_02970"
     CDS             3120..3545
                     /locus_tag="PLU99_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16049.1"
                     /translation="MIDPQDVPEVDPNETLARFILSRSHIRRSDGTIKPDAFVPHPHA
                     ELSVMRHRDATESELWAAGHAIAALRDRQLHGRGDVGADAFLQRGLAVLPAPIVNDAL
                     LPDNPNHANVTGWPKADPARQRLLALEIAAQAKLLWPDA"
     gene            3766..5952
                     /locus_tag="PLU99_02975"
     CDS             3766..5952
                     /locus_tag="PLU99_02975"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HOO16050.1"
                     /translation="MFKPLTAKACRRIWQRPSLAAVLLLLGAAAPLAQATWSIVIADS
                     RTKEVAAGTVTCLNDYDLLGLVPVIVVGKGAGACQASGDFDGLRRPIIFNQLALGTPP
                     NQILTMLSSVSGHNARQYGIADTQGRMMTFTGGACHAWRGGVTGSDGSLVYTIQGNIL
                     AGDCVVPAIEAALLSTPGDIPAKLMAGMEAARAAGGDGRCSCTVGGPMDCGCPPEAFA
                     KSGHIGGMVVARIGDTDDPVCDVSGCVDGNYFMRLNVAFQPSDAVDPVLQLAGLFAEW
                     RAGLTGRPDAIQSEASLELAPIPPDEVSTTVLHVRLRDWQGAAITGTIQSVDVAHSAG
                     SAGLSSIGPVNDLGGGEYVVTLTAGGRSGVDRFTISVDDGIRPVILMPETTLEYFPFG
                     DVNCDGAFNVLDIDPFVLALVSPAAYGAAHPECNQALADMNRDGQVNIFDIDAFVLLL
                     THDCNHNGVLDSQDILEGTSADCNGNGIPDECDLETGDSPDCNGNQVPDECDLAGGLS
                     ADCNENGVPDECDIGGGASHDCNENGAPDECDIAAGLSQDCQPNGIPDECDMAPPTYT
                     AAQDQCAAAELICPGHWMGGSTSAATNDGSASCGLSSASPDVWYFYQPAGNGYATISL
                     CGSAYDTVLSLHTGCPGTADNQLICNDNGCGLQSTLSYYVQSGHAYWIRVSGSNGAAG
                     EFDILLSGPACAFDGDCNDNGVPDDCDIAAGTSLDLDENGVPDECE"
     gene            complement(6000..8219)
                     /locus_tag="PLU99_02980"
     CDS             complement(6000..8219)
                     /locus_tag="PLU99_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16051.1"
                     /translation="MRGRLIPIGALAVAVALVYGATRFAPQIDAGRKELNVFGAEDVS
                     KNTPPQYAFAIQAFGAFRSLIVNIAFIRAETLKDEGRYYDAMQLASWICQLQPRFPTV
                     WAFHSWNMAWNISVSTFTPQERWKWVYNGAKLLRDQGIPLNPRAVTLYKELAWIFNNK
                     MGETMDDFHWEYKREWAWRMHLLLGPRPLPETTAPSDEQQPALIDVTDDPLTEVARRI
                     AEQNEAKRQERIAEEQVPFRTSKSIQELLERAEQDIAAVRVSQPAEMARRAVYEAIKS
                     IDEAPRSLEELYAKFPETREMVTRLREIGVVLSDDELSEEDYWYDGKLAANFFARYRQ
                     LVERPNLLTAIMRDSPDAVQEDEGLRLLDEILGVRAGNPAGQALVRCLQRKVLTQVYQ
                     MDPAHMAYVIENFGPVDWRSVDFQTLYWVSLGLIRGGESPTSFQTDRVNTARLIFFAL
                     RNLFNNNKIVFEPNPEEPFRSYMNTSIDVDFVPSMHRAYITYGPMLDPDPGNVTGAGS
                     IFRTGHINFLTEAVRALYFADREDEAEHYYAYLRTHYGFTEAEGQLDERYTLPLRDFV
                     LKSLYGSIETLRQTEIVIRSLLENAYNKLAEGDGVGYNQYVKKALEFHEVYQKEKADW
                     RTGRVTLLPFSNYQVDTLRQWLSFPGLRPELTLRKVHLWWNAPLTLRQSVYDELIPAL
                     ARECAYWDFDSAKAFPEPEGMEQYRKEHPRREGERQPGERAKERKGAETPAQSFIKD"
     gene            complement(8251..9642)
                     /locus_tag="PLU99_02985"
     CDS             complement(8251..9642)
                     /locus_tag="PLU99_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO16052.1"
                     /translation="MKNVLAIARLTFREGVRMRIVLVFVVVLGFLMLWLPFTVRGDQT
                     VTGQLQTFLSYSLGATGLLLGLAGVFLSCSTLTTEFRSMTLHLVLTKPVSRIEVLAGK
                     WLGINILLLLLLALCGAAIYGFAVLIKNRPASFERDRLNVRDVVWQARVAATPKPPPD
                     LEKEARQWVESELRQGREFSRGTEFAVAQRLKQMEREWRRIPPQHFALYDFEGLVEPR
                     DPETVFQVRFRANAKPMPLDEMIAIDFGFLDPETHAWLGEVHRTQERANIWHEFLARG
                     QGFIKDGRATLAVANPAPPTRSTAIVFDEEPWIQIMYTIGTFEESYLKVLLLIAGRLA
                     VLSALGLFFSVFVSFPVACFCVLTFYVICLGMPFWMEGIGANLELPVASVDPYGSFGP
                     AVRLLLVPLMKFVFPNFSEYSGVDQLIAGEYVSTWLVSKAMLHTAVYGAVLLFVPGWA
                     VFQRREIADVTVA"
     gene            complement(9639..10811)
                     /locus_tag="PLU99_02990"
     CDS             complement(9639..10811)
                     /locus_tag="PLU99_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16053.1"
                     /translation="MNTTAVIATVGLTKVFKDFWRRARVKAVSDLDLEIQPGEIFGLL
                     GPNGSGKSTTIKMILGLLNPTRGRISVFNRPPTDVRIKARIGYLPEESYLYRFLNARE
                     TLDYYGTLFHLPRRQRRQRTEELLEMVGLRSAARRPVGEYSKGMARRIGLAQALINDP
                     DLLILDEPTSGLDPIGAKLVKDIIVRIGRVLGKTILLSSHLLADVEEVCDRMTILYGG
                     KVQVTGPLDEVLSQRDRLQISCERLEPATLQAIQELVAAREGKQLSISPARDRLETLF
                     LRIVHEATARRVETAGSATTGQIAAFLGEKEQARAVLDSLVRVDEEPAAPAPAPPEET
                     VTVDDGVLRTLTASGQTAPQAAPEAARPAAPRPPMPDVDRGLLGELTQDTEPGDQA"
     gene            complement(10841..12124)
                     /locus_tag="PLU99_02995"
     CDS             complement(10841..12124)
                     /locus_tag="PLU99_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO16054.1"
                     /translation="METHNHNPSAPRGEFSSLPAAAPDLAQASLVGALRSSFAVLRLV
                     MYVLVILYAVSGVFRIEPGEQGVKVWLGQLVINEEKDSEFSGRPVFGPGWHWAWPDPF
                     SERICISGRTQEEESDTFLFKRSDKDIKDRTDIATIRRSTALKPGQDGAMLTGDKNLS
                     HGLWTAEYRISDAAKFVTQVGERVEDFAPMLRRLLDSSVLREVSYRKVEEVTRTKRDI
                     VAEAVRNRLQRELDELDLGVTIVKVTANTIVPPQVAAAFDDVAKAENEKKAQEDAARQ
                     RATEVLNQTAGPQYAELLEQIRAYGAAQLAGADAERLAELRHDIDDTLTRCQGLVAVR
                     LRDAQAKANTEREQLEAKYHEFVQRLEQYRAYPRQTLINLWTEMRREVLASKQNEIFW
                     VPETDFVDVIVNRDPNRAMEAERERLRREVLESAP"
     gene            complement(12111..13178)
                     /gene="hflC"
                     /locus_tag="PLU99_03000"
     CDS             complement(12111..13178)
                     /gene="hflC"
                     /locus_tag="PLU99_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOO16055.1"
                     /translation="MRRISLGTLVTAILVVVVLLVKLTTFMVRFSEVAVKVRMGQVAA
                     IVDKPGLYPRWPYPFESIKTYDTRLRTLDTLESEIKTRDGKNVIVGNYALWRIANPNT
                     FYIRVGNERDAEDKLRVRINQRRAAVIGNENLSAFVNLNQAAVDANYDRIENELLNGI
                     QTEGESAGLSLRDAVLADFGIDVVKVDLRRISLPEETTQTVFQQMSAERQKEAARFRE
                     EGKSRAETIRAQAEADRDQIIAFAERKAQEIQSTGIQAATGILKQINAEDAEFFEWLC
                     WLDALRDSLRQKSTIFLDSRSTMFDYFKAPVAPHPESGTASGRDAPLLKLPVPGPDFD
                     PLAPRTAEQPESSEGAARGNP"
     gene            complement(13234..15663)
                     /locus_tag="PLU99_03005"
     CDS             complement(13234..15663)
                     /locus_tag="PLU99_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO16056.1"
                     /translation="MTETPFTRSRRASLWGLVLQILAAAGIFALSEAAQSSAMFVLTW
                     FVAGGIPLWFVTLLVFRQRELAALEAMDLEELRREKKAIGAAEGMFDEEGGGGLAYRV
                     AEARLQWMQRWLVPAISLLHTAFLAGMGLFLWFRVGSDLPPLNAVHLPISMVVLTVIM
                     LLFFVFSRYASGMGREPQRQLLRACGSYALGNTLGALAVLVSLGVYKYAEVSAVERAV
                     GFALCALMIVLAAETLFSFVLEIYRPRMRGAEPRASFDSRLLGLISEPGGIASTIAEA
                     MNYQFGFEVSQTWFYQLLQKWLVPLLGAGLLILWLLTSVVVVQPGQHALIERFGRQLN
                     ADAPYPPGFYWKLPWPVDIACKYDTGNLHQINIGFKQFDARPSEDSQHKTGAALWTDE
                     RHFGQQHFNFLIPVPPGPQTVETGSELDDETAGLEQSWPVNTVRLDMVAQYRIDEKQL
                     ANYSRNCEKPHQAMLDVAWEETVRFAAAWDIFSLLGDKYGTAGQILKGRLQERVDELG
                     LGLEVVYVGMQNVHPDKVVATEFRKVVNAEQEKIAAIREALVKENEILSAVAGDRRRA
                     RELAQAIENITQPTIVQYQTAESLSQVDPALLASLRARFDELQPQFLKVISARWQLEE
                     DSARQREIELEFELGLGRNEAERGAAAGAVYESREQLLALEAELREAMEPVSKEAAAK
                     LGEQLADALLKNAEAGYALEFWNRRLERLLPGLQGEAAAILAAAQAERWMQEMRAAQQ
                     VARLAESVAYRAAPRLYQVRKYLAVLVEGIQNTRKFFLAFDPAGRQVRVRYLAEEQAA
                     PAGAEIETRMR"
     gene            15890..16870
                     /locus_tag="PLU99_03010"
     CDS             15890..16870
                     /locus_tag="PLU99_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO16057.1"
                     /translation="MQKLLAITRNTFVETTRQPIYSVLLWAAAFWVGFVSPALAGFTL
                     EVGNDKKVMIDVSLATLLLYGLLASVFSASSVITREIESHTVLTVVSKPVSRPLFLLG
                     KYLGVTGAVLLGYYFLCLVLLMAARHGTMETAAEKFDQPVLVFGLSALAIALIVSAFG
                     NYVYGWHFTSTLMAWAVPLVSLALLLVLFIDPEWKPQSPAKDFGDFQLIYAEISVFCG
                     VLILTAFAVALATRFSLVVTLMLCAAIFLLGLLSDYYFGGPAMEGRGPLYSLLYGAVP
                     NFQYFWLGDPITQQLTIPGRQVAAVAGYASLYALAVLALGVSLFQTREVG"
     gene            complement(16917..18890)
                     /locus_tag="PLU99_03015"
     CDS             complement(16917..18890)
                     /locus_tag="PLU99_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOO16058.1"
                     /translation="MKSETGRATLKLIATLLGGMLVLMSFVAQWAFKGEVAPGDDSGR
                     NIYAGLLAAMGALLLAVPLWVHAAKCLFGGHMHMDELVALAILAAMAIGEYQTAGVVA
                     FFLLLSNLIETRTALGARAAIEGLMRLTPTKAHRILPDGSEERVDAHALRAGDVVHIR
                     PGDNIPADGVILSGGSTINEANITGESLPVEKSAGDDVFGGTTNLTGALQVKVTKVGH
                     DTTLGRVQELILDAERTKIPLMRMIDQYAAWYTPVTLMIAGVVWFFTRDMTVTISLLV
                     IACPCALILATPTAVVAALSAAARLGIYIKDVSNLEWARKLTAVVFDKTGTLTTGELA
                     VTRLMPAPGVDAADLLRTAASVEQYSRHPVARAIATVAQRARVPLEEATDFAEAPGKG
                     VSGRLNGDIVSVGRRTWVAEQGVDLGPMDDEKYREPEGLSTLYVVRGTQCLGWIGLED
                     RTRPEAHESLDELRHLGIRQLIMVTGDRWSVARRVAKEMGCSDVQAEVLPQDKLELVS
                     ALQARGHTVAVVGDGVNDAPALAAGNLGIAMGAAGSDIAMNSAGVALMSNDLRRLPFL
                     VNLSRETTNVIWQNLIFGVTFILVAEVAIVYKFVGPMMAAFLHTLSSAIVVFNSARMV
                     RFGEHLEAHKPAVPRRAAMETEAAGRVGLQPVG"
     gene            complement(19595..19816)
                     /locus_tag="PLU99_03020"
     CDS             complement(19595..19816)
                     /locus_tag="PLU99_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOO16059.1"
                     /translation="MSVDRSLRRKSSLERHRNVLSRAERVARLITEERFAEDSSPLGL
                     PKVAHRKAHAGKKKVKKEEGTAEGAAPSA"
     gene            20212..20682
                     /locus_tag="PLU99_03025"
     CDS             20212..20682
                     /locus_tag="PLU99_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO16060.1"
                     /translation="MNQRIQFQNVAPEALQAIYGLEKYLRKSGLERALLDLVKLRASQ
                     LNGCAYCVDMHSKDLRAAGESEERLFALPVWRESPYYTERERAALAWAEALTNIQDGH
                     APDDLYALVRQQFSEKELVDLTMAIVAINGWNRLAIAFRVPAGTYQPAHHAVKT"
     gene            complement(20698..21609)
                     /locus_tag="PLU99_03030"
     CDS             complement(20698..21609)
                     /locus_tag="PLU99_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOO16061.1"
                     /translation="MAGFFLRPVAPFRLDLTVWALRRVPINLIDRWDGQTYRRVVPVR
                     GRPVEVAVEQVAGGDAPRLRVCTSERRIGARDREVLVPLLERVLGLRADLREFHRRVG
                     EHKDFAPLVAPFIGLKPPRFPSVFEAVLNGIACQQLSLIVGIHLLNRLSATFGPAVGD
                     QHAFPRPEDLAGQRRARIRQLGFSGRKVETIRWLARAVVRGELNLEELATKEDAVAFK
                     ALLNLTGIGRWTAEYVLLRGLGRWDTFPADDVGSQSKLQSWLHLEKRPNYDGVQQILA
                     SCKPYRGLIYFFLLLNQLRAAHLLESS"
     gene            complement(21743..>22131)
                     /locus_tag="PLU99_03035"
     CDS             complement(21743..>22131)
                     /locus_tag="PLU99_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16062.1"
                     /translation="ARDAAAALDTAGPLERLLHIHVYWQPYPGKTFSNSSTTTATIRY
                     VVATREGTVSYTGTGFAYPAKRSGDGLEIDLESASLQLDTSTGAAPEMLGISRLSGKL
                     FARHDPHRTVDLMRQVALLAAGNTLP"
BASE COUNT         3881 a         7167 c         6953 g         4130 t
ORIGIN      
        1 ccggccttgc tgtcggtgcc gctcatgttg aagccgccga agggctgcac gtcgaccagc
       61 gcgccggtgc acttacgatt gaagtacaga ttgcccacgt ggaattcgtg gcgggcgcgc
      121 tccaggcgga agcggtcgtt ggagtacagg gcgccggtca ggccgaaacg cgtgccattc
      181 acgatcgcca agccgtcatc gaaatccttg gctttgatga ccgccagtac cggcccgaag
      241 atttcctcct gggcgatgcg cgcgttgcgg tcgacgtcgg cgatgatcgt cggcggaacg
      301 aagtagccgc cggccggcac gctggtcccg cccagcacct gccgcccttc cttcttgccg
      361 atcgcgatgt aatcgttgat cttcttgaaa gaggcgtcgt cgatcaccgc ccccatgtag
      421 aggttctctt cgctggtcgt atcgccgatg gtgagcttct tggcccgctc gaccacgcgc
      481 tcgagcaccg cgtcgtagac gtcctggtgc aggatggccc gcgagcaggc cgagcacttc
      541 tgcccctgga aaccgaaggc ggaggcgacg atgccctcgg ccgcggcttc cacgtcggcg
      601 gtttcatcga ccaggatgca atccttgccg cccatctcca gcacggagcg cttcagccag
      661 atctggtctt tgccgaccat gccgcacttc tggaagatgt tgagcccgac ttccatggag
      721 ccggtgaacg agacgaagcg cgtcttggga tgctcgacca ggtagtcgcc gatttcgccg
      781 cccgagccgg ggcagaaatt gagcacgccc ggcggcagcc cggcctcttc gaatatctca
      841 accatcttgg ccgcgatgac cggcgaagtg ctggccggct tgagcaccac cgtgttgccc
      901 gtcacgatcg cggccgacgt catgccggtg cagatcgcca acgggaagtt ccacggcgga
      961 atcaccacgc ccacccccag tggcatgtag agcaactcgt tgtcttcgcc cggatacggc
     1021 gtcaccggct gcggaccacc cagccggacc atctcgcgcc cgtagaactc aaggaagtcg
     1081 atggcctcgc aggcgtcggc gtaggcttcg atccagctct tggacacttc aaagcacatc
     1141 caggcggcca cttcgtagac gcggcggcgc atgatggccg cggctttgaa caggatgcgc
     1201 gcccgcgttt cggggtcgac gcggctccag gtggggaaag ttttgagcgc ggcctccatg
     1261 gcctgggcgg cctgctcacg gccggctttt gaaacgtggc cgacgacggt ttcgtggcgg
     1321 gccggcatga cggaggcgat tttcttttcg gtttcgatct ttttgccccc gatgcggagc
     1381 gggtaagtgc ggccaagctg ctggcgcact tgctggaggg cggcgagcat ctgctggcgc
     1441 gggccgggtt cggtgaaatt aacgtatggt tcggggcgat agggtaccaa catggcagcg
     1501 actcccttca aaacagtgac tacgactagt tcgtttgtcc accgcccccg ccgggcgggg
     1561 tacaaccggt cgcacccgct gaggcgcaaa ctcacattgt agcacaaaaa cgcggcgggc
     1621 caaaccgcgg cgcgccggcg gtcgcgtgtg ccgagccact tcgacgcatc cgttggcact
     1681 cgcgcatccc ggtcgaagga gcttcgagct tggcggacgg ttttggcctc aacgtccccg
     1741 gcatattcgc cgacgttgtg taagagatgg cttgacaaga ggagggcgga taagatctta
     1801 ggtgttgtac gagacgcgag cccatggcgg cacagcccgt attcaagctg gacctgtcgg
     1861 agcgatttcc gcacttgccc cgcgcgccga tagtcgaggc ggtcattcac tggcgcgccc
     1921 gcagcgaaca aaggcttgcg cgtgaagacc tgcggcgcga actgacgtcg gcgcttccag
     1981 actatccgac gatcaagacg cagcatgagc tacactttga atccgcactc ggtcccgacg
     2041 gcgccgccgt ctcgagccgc gacgaatggc atggcttccg cctggaatcc acggacgggc
     2101 ggtacatcgc gcaattcacc cgcggtggct tcgttttcag tcgggtaacc ccttatgaaa
     2161 actgggaccg ttttgccgcc gaagccaaac gcctatggca gatccatgtg agcttggcca
     2221 aaccgtccga gatcgagcgc ctgggcgtgc gattcatcaa ccggattgtg cctgtggacc
     2281 tcagcaggct ggacgacatc ctggtgctgc cgccacaaac gccgcccggc ctgccgctgc
     2341 cgatcgatga gttcctgcag cgtagcctct tcgacgtccc tggccatccg taccgcgtca
     2401 atgtcatcca gacgacgcag ccgcccatac cgcccgaaac cgataccctc ggtcttattc
     2461 tggacatcga tgtgttcacg acggctagca tccccctgaa cgacgcgctg gccgatacgc
     2521 gcctggctga aatgcgctgg ctgaagaaca aggttttctt ctcgctgatc agtccgcagg
     2581 caaccgcccg cttccaggag gacgatgcgt gagacttgcc atcagcggct acggagtcag
     2641 cagcgcggcc cggtacatcg acgcgaccac acgccgcaac cgccgccatc tgcaccagtc
     2701 cccggctttt ggcatcgaaa gcgttttgcg tgacgacctt gcggcggctt gggccgaatg
     2761 ccgtcagccc ggctgggacg ggtacgacgc tctccccgtg gcgcaggacg ctctgcggaa
     2821 catgtacata ttcctggagg cgctgccgct ggggtctccg cacccgacga tcgcggccga
     2881 tccgcacgga tatttctcag ccgagtggta ccgcggaccg cggcgcgtgc tctcagtcgc
     2941 ggtcacggac gacgacctcc tgcattatgc ggccctggtt ggccccaacc gggtttgcgg
     3001 tacagaaaca ttctacggcg aaattcccga agccatcctc agcctgatgc ggcgagttta
     3061 ttcataatcg gtgtaatgcc gcgtttttgc acgatggacc taatgcgccg agtttcttga
     3121 tgattgaccc gcaagatgtg ccggaggtcg acccgaacga aacgttagcg cgtttcattt
     3181 tatcgcggag tcacatacgc cgcagtgatg ggaccatcaa gcccgacgcc ttcgttcctc
     3241 acccgcacgc cgagctctcg gtaatgcgcc atcgcgatgc tactgaatcc gagctctggg
     3301 cagccggcca cgcaatcgcg gcgctccgcg accgccagct tcacggacgc ggggacgtcg
     3361 gcgcggacgc attcctgcag cgcggcctcg cggttctccc cgcgccgatc gtcaacgacg
     3421 ccctcctgcc cgataaccca aaccacgcca acgtaaccgg ctggcccaaa gccgatccgg
     3481 cccgccagcg tctcctcgcc ctcgagatcg ctgcgcaggc caagctcctc tggcccgatg
     3541 cgtgacgcaa ataccagcat agggtctgcg gctcgcaggc cggccctcag cactgcgaac
     3601 aaatagcgct ccgatcggcc gcggtgcgct gtagaattgt cacggggccc ggctggcggc
     3661 gcgagagcga ggtttgtggc gccggcgtat ggccggtgaa ccaaacccgc ggcaccgatg
     3721 tttcgccggc gaagccggca gtcaggcccg tcttggaggt gaagcatgtt caaaccgctt
     3781 acggcgaaag cttgtcgcag aatatggcag cgaccgagtc tggctgcggt gttgttgctg
     3841 ttgggcgcgg ccgcgccgct cgctcaggcc acctggtcaa tcgtcatcgc cgatagccgc
     3901 acgaaagagg tcgccgccgg cacggtcacc tgcctgaatg actacgacct gctcggtctg
     3961 gtgccggtga tagtagtcgg caaaggcgcc ggggcgtgcc aggcctcggg cgacttcgac
     4021 ggcctgcgcc ggccaatcat cttcaaccag ttggctctcg gcacgccccc gaaccagata
     4081 ctgacgatgc tctcgtcggt gagcgggcac aacgcgcggc aatatggaat cgccgacacg
     4141 cagggccgaa tgatgacgtt taccggcggc gcgtgtcacg cctggcgcgg cggcgtgacc
     4201 ggcagcgacg gctcactggt ctacacgatt cagggcaaca ttctggctgg cgattgcgtc
     4261 gtgcctgcta tcgaggcggc cctgctaagc acgcccggcg acattccggc gaagcttatg
     4321 gccggcatgg aggccgcccg cgcggccggc ggcgacgggc gctgttcgtg cactgtgggt
     4381 gggccgatgg actgcggctg tccgccggag gcttttgcca agtcgggtca cataggcggg
     4441 atggttgtgg cgcgcatcgg ggatacggat gatccagtct gcgacgtgag cggctgcgtc
     4501 gacggcaatt actttatgcg cttgaatgtg gcatttcagc cgagcgatgc ggtagacccg
     4561 gtcttgcagc ttgcgggcct gtttgcggag tggcgcgcgg ggctgacggg gcggcctgac
     4621 gcgattcaat ctgaagcaag cctggagcta gcgcccattc cgccggacga agtctccacg
     4681 acggtgctac atgtccgctt gcgtgactgg cagggtgcgg cgattacggg gacgattcag
     4741 agcgtggacg tggcccacag cgccggcagc gccgggcttt caagcatcgg gccggtgaat
     4801 gatctgggtg gcggagagta cgtcgtgacg ctgacggcgg gtggacgctc gggcgtcgac
     4861 cggtttacca tcagcgtgga cgacggcatc cgccccgtaa tcctgatgcc tgagacgacg
     4921 cttgagtact ttcccttcgg cgacgtcaat tgcgacggcg cattcaatgt tcttgacatc
     4981 gacccgtttg tgcttgcgct ggtaagcccc gcggcctatg gggcggcgca tccggaatgc
     5041 aatcaggccc tggccgacat gaaccgtgac gggcaagtca acatttttga catcgacgcg
     5101 ttcgtgctgt tgctgacgca cgactgcaat cacaacggcg tgcttgattc gcaggacatc
     5161 ctggagggca caagtgcgga ctgcaatggt aacggcatac ccgacgagtg cgacctggaa
     5221 acgggcgaca gcccggactg caacggcaac caggttccgg atgaatgcga tctcgccggc
     5281 gggctgagtg cggactgcaa tgagaacggc gtgccggacg agtgcgacat cggcggcggc
     5341 gccagccacg actgtaacga gaacggcgcg ccggacgagt gcgacatcgc cgccggtctg
     5401 agccaggatt gccagccgaa cggaattccc gacgagtgcg acatggctcc gccgacctac
     5461 acggccgcgc aggatcagtg cgccgcggcc gagctgatct gtccggggca ctggatgggc
     5521 ggctcgacca gcgcggcgac gaacgacggt tccgcgtcct gcggcctgtc gagcgcttcg
     5581 ccggatgtgt ggtacttcta ccagccggcc ggtaacgggt acgcaaccat ctcactgtgc
     5641 ggctcggcgt acgacacggt gctgtcctta cacactggtt gtcccggcac ggcggacaat
     5701 caactgatct gcaacgacaa tggctgtgga cttcagtcca cgctgtctta ctacgtccaa
     5761 tccgggcatg cgtactggat cagggtctcg ggcagtaatg gggccgcggg cgaattcgat
     5821 attctgctga gcggtccggc gtgtgcattc gacggcgact gcaacgacaa cggtgttccg
     5881 gacgattgcg acattgccgc cggcacgtcg cttgatctgg atgaaaacgg cgtgccggat
     5941 gaatgcgagt agccgggcgg aagaaggcct ttctgcccgc tgacctttac ttctgtccgc
     6001 tagtctttaa tgaaggactg agccggggtc tcggcgccct tgcgctcctt ggcccgctct
     6061 ccgggctggc gctcaccctc gcgccgcgga tgctccttgc ggtactgctc cataccttcc
     6121 ggttcgggga aggccttggc cgagtcgaaa tcccagtagg cacactcccg cgccaaagcc
     6181 gggatcagct cgtcgtagac ggactgccgc aaggtgagcg gggcattcca ccacaagtgt
     6241 actttgcgca aggtcagttc cggacggagg cccgggaacg agagccattg ccgcagcgtg
     6301 tccacctggt aattgctgaa gggcagcagc gtcacgcggc cggtgcgcca atccgccttc
     6361 tctttctggt acacctcgtg gaactcgagc gccttcttga catactggtt gtagccgacc
     6421 ccgtcgcctt cggcgagctt gttgtaggcg ttctccagca ggctgcgaat cacgatctcg
     6481 gtctggcgca gcgtctcgat tgaaccgtac aggctcttca gcacgaaatc gcggagcggc
     6541 agcgtgtagc gctcgtcgag ttgcccctcg gcttcggtaa aaccgtagtg cgtgcgcaaa
     6601 taggcatagt aatgttcggc ctcatcctcg cggtcggcga agtacaaggc gcgcacggcc
     6661 tcggtcagga aattgatgtg tcccgtgcgg aatatcgaac ccgccccggt gacgttgccc
     6721 ggatcggggt cgagcatcgg cccgtacgtg atgtaggcgc ggtgcatgga gggcacgaag
     6781 tcgacgtcga tcgaggtgtt catgtacgag cggaacggct cctccgggtt cggctcgaag
     6841 actattttgt tgttgttaaa caggtttcgc agggcgaaaa agatcagccg cgccgtatta
     6901 accctgtccg tctggaacga cgtcgggctc tcgccgccgc gaatcaggcc caggcttacc
     6961 cagtagaggg tctgaaaatc cacgctgcgc cagtcgaccg gcccgaagtt ctcaattacg
     7021 tacgccatat gggccggatc catctggtac acttgagtga ggactttgcg ctgcaggcag
     7081 cgcaccaggg cctgccccgc cgggttgccg gcgcgcactc cgagaatctc gtccagcagg
     7141 cggagacctt cgtcttcctg caccgcgtcc ggactgtccc gcattatggc cgtcagcagg
     7201 ttgggcctct cgacaagctg ccggtaacga gcgaagaagt tggccgccag cttgccgtca
     7261 taccaatagt cctcttcgct cagttcgtcg tcggagagca ctacgccgat ctcccgcagg
     7321 cgcgtcacca tctcacgcgt ttcggggaat ttggcgtaaa gctcctcgag cgaccgcggg
     7381 gcttcgtcaa tgctcttgat ggcctcgtac acggcgcgcc gcgccatctc ggccggctgg
     7441 gagacgcgca cggccgcaat gtcctgctcg gcccgctcca gcagttcctg aatcgacttt
     7501 gaagtgcgga acggcacctg ctcctcggcg atgcgttctt gacgcttggc ctcattctgt
     7561 tcggcgatgc ggcgggccac ttcagtgagc gggtcgtcgg ttacgtcgat cagggccggc
     7621 tgctgctcgt cggagggcgc ggtggtttcc ggcagtggcc gcggccccag cagcaggtgc
     7681 atgcgccagg cccattcgcg tttgtattcc cagtggaaat cgtccatggt ctcgcccatc
     7741 ttgttgttga agatccaggc gagttccttg tacagcgtga cggcgcgcgg attcagcgga
     7801 atgccctgat cgcgcagcag cttcgcgccg ttgtagaccc atttccagcg ttcttgcggg
     7861 gtgaaagtgc tgaccgagat gttccaggcc atgttccagg agtggaaggc ccacaccgtc
     7921 ggaaagcgcg gttgaagctg gcaaatccaa gaagccaact gcatggcgtc gtaatagcgg
     7981 ccttcgtcct tgagcgtttc ggcccggatg aaggcgatgt tgacgatcaa gctgcggaac
     8041 gcgccgaagg cctgtatcgc gaaggcgtac tgcggcggcg tgttcttgga aacgtcctcg
     8101 gcgccgaaca cgttcagttc tttgcggccg gcgtctatct ggggcgcgaa gcgcgtggcg
     8161 ccgtaaacca gggcgaccgc caccgccaaa gcgccgattg ggatcagtct gccacgcatc
     8221 cgcatgtccg ccaacagtta aggggtgctc tcatgcgacc gtcacgtccg ctatctcgcg
     8281 ccgctggaac accgcccagc ccggcacgaa caacaacacg gccccgtaga ccgccgtgtg
     8341 aagcatggcc ttggaaacca gccacgtgga cacgtattcg ccggcgataa gctgatcgac
     8401 gccgctgtac tcggaaaaat tgggaaacac aaatttcatc agcggcacca gcagcagccg
     8461 cacggccggc ccgaagctgc cgtacgggtc gaccgaggcg accggcaact ccagattggc
     8521 cccgatgcct tccatccaga acggcatgcc caggcagatt acgtagaagg tcaatacgca
     8581 gaagcaagcc acggggaagg acacgaacac gctgaagaac aagcccaggg cgctgaggac
     8641 cgccaggcgc ccggcgatca acagcagcac cttgagatag ctctcttcga acgtgccgat
     8701 ggtatacatt atctgtatcc agggctcctc atcgaaaacg atggcggtgc tccgggtggg
     8761 cggcgcagga ttggccacgg ccagcgtggc gcgcccgtcc ttgatgaagc cctggccgcg
     8821 ggccaggaac tcatgccaga tattggcgcg ctcctgcgtg cggtgtactt cgcccagcca
     8881 ggcgtgcgtc tcggggtcga ggaagccaaa atcaatggcg atcatttcgt ccagaggcat
     8941 gggcttggca ttggcccgaa agcggacttg gaacacggtt tccgggtcgc gcggctcgac
     9001 cagtccctcg aaatcgtaca acgcgaagtg ctgcgggggg attctccgcc attcacgttc
     9061 catttgcttg aggcgctgcg cgacggcgaa ttccgtgccg cgcgagaact cgcgcccctg
     9121 ccgcagttct gattcgaccc actgacgggc ctctttctcc aagtcgggcg gcggcttagg
     9181 ggtggccgcc acgcgggctt gccacacgac gtcccgtacg ttgagccggt cgcgctcgaa
     9241 ggaggcgggc cggttcttga tcaacaccgc gaagccgtaa attgccgcgc cgcacaacgc
     9301 cagcagcagc agcaagagga tgtttatgcc gagccacttg cccgccagca cttcgatgcg
     9361 cgaaacgggc ttggtgagca ccaggtgcag cgtcatgctg cggaattcgg tggtgagcgt
     9421 ggagcacgac aaaaacacgc ccgccaggcc cagcagcagc cccgttgcgc ccagcgagta
     9481 tgacaagaag gtctgcaact ggccggtgac ggtttggtcg ccgcgcaccg taaacggcag
     9541 ccagagcatc aagaacccca gcacgaccac gaacaccagg acgatgcgca tgcgaacgcc
     9601 ctcgcgaaac gtcaggcggg cgatcgccag cacgttcttc atgcctggtc tcctggctcc
     9661 gtatcctggg tcagctcgcc tagcaacccg cggtcgacgt ccggcatggg cgggcggggc
     9721 gcggcagggc gggccgcctc cggggcggcc tgcggcgccg tctggccgga agcggtgagc
     9781 gtgcgcagta ccccgtcatc caccgttacc gtttcctcgg gcggggccgg cgcaggcgcg
     9841 gccggctcct cgtcgacacg caccaacgaa tccagcacgg cgcgggcctg ttctttctcg
     9901 cccaagaatg cagcgatctg gccggtcgtg gcggagccgg ccgtttccac gcggcgcgcg
     9961 gttgcttcgt gcacgattcg caggaacagg gtttccagcc gatcgcgcgc cgggctgatg
    10021 ctcaactgtt tgccctcgcg cgcggcgacc agttcctgga tggcttgcag cgtggcgggt
    10081 tcgagccgtt cgcagcttat ctgcagccgg tcgcgctggc tcaggacctc gtccagcggt
    10141 ccggttactt ggacctttcc gccgtacagg atggtcatgc ggtcgcagac ctcctcgacg
    10201 tcggccaaca ggtgactgga gagcaagatg gtcttcccca gcacgcgccc gatgcggacg
    10261 atgatgtctt tgaccagctt ggcgccaatc gggtcgagtc cgctggtcgg ctcgtccaga
    10321 atcagcagat ccgggtcatt gatgagcgcc tgggccaagc cgatccggcg ggccatgccc
    10381 ttggaatact cgccgaccgg gcggcgggcg gcggatctga ggccgaccat ttcgagcaac
    10441 tcttcggtgc gttggcgacg ctgccggcgc ggcaggtgaa agagcgtgcc gtagtagtcc
    10501 agcgtctcgc gcgcgttgag aaagcggtac aggtacgatt cttccggcaa atagccgatg
    10561 cgggccttga tgcgcacgtc ggtgggcgga cggttgaaga ccgagattcg cccgcgggtg
    10621 gggttcaaaa gcccgaggat catcttgatg gtggtgcttt tgccggaacc attaggtccc
    10681 aacaggccga agatttcgcc gggctgaatc tccagatcca aatcgctgac ggccttgacg
    10741 cgggcccggc gccagaagtc cttaaagacc ttggtcagtc cgacggtggc gatgacggcg
    10801 gtggtattca cattcgtcct tcaagccggc ggcaggccgc ttatggagcg ctttctagaa
    10861 cctcgcggcg cagtcgctcg cgctcggcct ccatggcacg gttcgggtcg cggttgacga
    10921 tgacgtcgac aaagtcggtc tcgggcaccc agaagatttc attctgcttg ctggccagca
    10981 cctctcggcg catctccgtc cagagattga tcaacgtctg gcgcgggtag gcccggtatt
    11041 gctcgagccg ctggacaaac tcgtggtatt tggcctcgag ttgctcacgt tcggtgttgg
    11101 ccttggcctg cgcgtcgcgc agccggacgg ccaccagccc ctgacagcgg gtcagcgtgt
    11161 cgtcaatgtc gtggcgcagc tcggccagcc gctccgcatc ggcgccggcc aactgtgcgg
    11221 cgccgtaggc gcgaatctgc tccagcagtt cggcgtactg cgggccggcg gtctggttga
    11281 ggacttcggt ggccctctgc cgggccgcgt cctcctgggc ctttttctcg ttttcggcct
    11341 tggcgacgtc gtcaaaggcg gcggcgacct gcgggggcac gatggtgttg gcggtcacct
    11401 ttacgatggt cacccccaag tccagttcgt ccagctcgcg ctgcagccgg ttgcggaccg
    11461 cttcggccac gatgtcgcgc ttggtgcgtg tgacttcttc gaccttgcga tacgaaacct
    11521 cgcgcagcac cgagctatcg agcaagcggc gcagcatggg cgcgaaatcc tcgacccgtt
    11581 cgccgacctg cgtcacgaat ttggcggcgt cgctgatgcg gtactcggcc gtccacagtc
    11641 cgtgcgagag gttcttgtct cctgtgagca tggccccatc ctggccgggt ttgagcgcgg
    11701 tgctgcggcg gatggtggcg atgtcggtgc ggtccttgat gtccttgtcg ctgcgtttga
    11761 ataggaaggt gtcgctctct tcctcctgag ttctgccgga gatgcagatg cgttcggaga
    11821 aaggatcggg ccaggcccag tgccagcccg gaccaaacac cgggcggccg ctgaactcgc
    11881 tgtctttctc ctcgttgatg accaactggc ccagccagac cttgacgccc tgttcgcccg
    11941 gttcgatgcg gaagacgccc gacacggcgt acaaaatgac cagcacgtac atgaccagcc
    12001 gcaagacggc gaagctggag cgcagcgccc cgaccagaga agcctgcgcc aaatcgggcg
    12061 cggcggccgg cagggagctg aattcgccgc gtggcgcgga tggattgtgg ttatgggttt
    12121 ccacgcgcgg ctccttcgct ggactcgggt tgctcggcgg tacgcggcgc cagcgggtcg
    12181 aaatccggcc cgggcaccgg caacttcagc agcggcgcat cccgcccgga agccgtcccg
    12241 gattcagggt gcggcgcgac cggggccttg aaatagtcaa acatcgttga gcggctatcc
    12301 aggaagatgg tgctcttctg cctgagcgaa tcgcgcagcg cgtccagcca gcagagccat
    12361 tcgaagaatt cggcgtcttc ggcgtttatc tgcttgagaa ttccggtggc ggcctggatg
    12421 ccggtggact gaatctcctg tgctttgcgc tcggcgaaag cgatgatctg gtcgcggtcg
    12481 gcctcggcct gggccctgat ggtctcggcg cgtgacttac cttcctcgcg gaagcgggcg
    12541 gcttccttct ggcgttcggc gctcatctgc tggaagacgg tctgcgtggt ctcctccggc
    12601 agcgagatgc ggcgcagatc gaccttgacc acgtcgatgc cgaagtccgc cagcacggcg
    12661 tcgcgcagcg acagcccggc gctctcacct tccgtctgga tgccgttcag caactcgttt
    12721 tcaatgcgat cgtagttggc gtccacggcc gcctggttga gattgacgaa cgccgaaagg
    12781 ttctcgttgc cgatgaccgc cgcgcgccgc tgattgatgc gcacacgcag tttgtcttcc
    12841 gcgtcacgct cattgccgac ccggatgtaa aacgtgttcg ggttggcgat gcgccacagc
    12901 gcgtagttgc ccacgatcac gttcttcccg tcacgcgtct tgatctcgct ttccagcgta
    12961 tccagcgtgc gcagccgcgt gtcgtaggtc ttgatcgatt cgaacgggta cggccagcgc
    13021 gggtacagcc ccggcttatc gacgatggcc gccacctggc ccatgcggac cttcacggcc
    13081 acctccgaga agcgcaccat gaaggtcgtc agtttcacca gcagcaccac cactaccaga
    13141 atggcggtta ccagtgtgcc taatgagatg cgtctcatat ttgctccttg ccggccgctt
    13201 gcagccggcg cttttgtgcg ctgcgtatca aggtcatctc atgcgcgtct cgatttcggc
    13261 tcccgcgggc gccgcctgct cttcggccag gtagcgcacg cgcacctgtc gcccggccgg
    13321 gtcgaaggcc aggaagaact tgcgcgtgtt ctgaatgcct tccacgagca ccgccaggta
    13381 cttccgcacc tggtacagcc gcggcgctgc gcgataggct acgctttcgg ccaggcgggc
    13441 tacctgctga gcggcgcgca tttcctgcat ccagcgctcg gcctgcgccg ccgccagaat
    13501 ggcggccgcc tcgccttgca gccccggcag cagccgctcc aggcggcggt tccagaactc
    13561 cagggcatag ccggcttcgg cgtttttcag cagggcgtcg gccagttgtt cgcccagctt
    13621 ggcggcggcc tccttcgaaa ccggttccat ggcctcccgc aattccgctt ccagcgccaa
    13681 gagctgctcg cgcgactcat atacggctcc ggccgccgcg ccgcgttccg cctcgttgcg
    13741 ccccagcccc aattcgaact cgagttcgat ctcccgctgc ctggcgctgt cctcctccag
    13801 ttgccaacgg gcgctgatca ctttcaaaaa ctgcggctgc agttcgtcga agcgggcccg
    13861 caggctggcc agcaacgcgg gatcgacttg cgataagctt tccgccgtct ggtactgcac
    13921 gattgtgggc tgcgtgatgt tctcgatcgc ctgggccagt tcccgggccc gccggcggtc
    13981 gccggccaca gccgacagta tttcgttttc tttcaccagc gcttcgcgaa tggccgcgat
    14041 cttctcttgc tccgcgttca ccactttgcg gaattcggtc gccaccacct tgtcgggatg
    14101 cacgttttgc atgccgacgt agaccacttc caggcccagg cccaactcgt ccactcgctc
    14161 ttgcagacgc cccttcaaaa tctgccccgc cgtgccgtac ttgtccccca gcagcgagaa
    14221 aatgtcccaa gcggccgcaa aacgaacggt ttcctcccag gccacgtcca gcattgcctg
    14281 gtgcggcttc tcacaattac ggctgtagtt ggcgagctgc ttctcgtcga tccggtattg
    14341 cgccaccatg tccagccgga ccgtgttcac gggccaggac tgttcgagcc cggcggtctc
    14401 gtcgtccagt tccgatcccg tttccacggt ctgcgggccg ggcggtaccg ggatcaggaa
    14461 gttgaagtgc tgttggccga agtgtcgttc atcggtccac agtgccgcgc ccgtcttgtg
    14521 ctgcgaatcc tcgctgggcc gggcgtcaaa ctgcttgaag ccgatgttga tctggtgcag
    14581 gttgccggtg tcgtatttgc acgcgatgtc gaccggccag ggcagcttcc aatagaagcc
    14641 gggcggatag ggcgcgtcgg cgttgagttg ccgcccaaag cgctcgatca acgcgtgctg
    14701 cccgggttga accacaacca cggaggtcaa cagccagaga atcagcagcc ccgcccccag
    14761 caacggcacc agccacttct gcaagagctg gtagaaccaa gtctgcgaaa cctcgaagcc
    14821 gaactggtaa ttcatggctt cggcgatggt cgaggctatg ccgccgggtt cggagatcaa
    14881 ccccagcaac cggctgtcga agctggcgcg cggctcggcg ccgcgcatgc gcgggcggta
    14941 gatctccagc acgaacgaga agagcgtttc ggcggccagt acgatcatca gggcgcaaag
    15001 cgcgaagccg accgcgcgct cgaccgccga cacctcggcg tacttgtaaa ctcctaacga
    15061 gaccagcacc gccagcgcgc caagagtgtt gccgagggcg tatgacccac aggcccgcag
    15121 aagctgccgc tgaggctcgc gtcccatgcc cgaggcgtaa cgcgagaaca cgaagaacag
    15181 cagcatgatg accgtgagga cgaccatcga gatcggcaga tggaccgcat tcaacggcgg
    15241 taggtcgctc cccacccgga accacaggaa cagccccatc cccgccagga aagcggtgtg
    15301 caacaagctg atggccggca ccagccagcg ctgcatccac tgtaggcgag cctccgcgac
    15361 gcggtatgcc agcccgcccc cgccttcctc gtcgaacatg ccttcggccg cgccgatggc
    15421 cttcttttcg cgccgcagtt cctcgaggtc catagcctcc agcgccgcca gttcgcgctg
    15481 ccggaatacc agcagtgtta cgaaccacag cgggatgccg cccgcgacga accacgtgag
    15541 cacgaacatc gctgacgact gagctgcttc cgacagggca aatatgccgg ccgcagccag
    15601 aatttgcagc accagacccc acaggctggc ccggcgactg cgggtaaacg gcgtttcagt
    15661 cacgattcac ctcttctgta tgcggcaccg gtcctgtatg caggaccggt ttgcagccgg
    15721 tcaatcggct attccagcgg acccttttcg aaccgtagcg gtccgcccgc gtaccccagc
    15781 ttgtccgagt gatggctcgc ggcaataatg cgcccccgaa attggccgct gcggggctgc
    15841 gagcccgccc ggcggcacga ttttcacggc aggacggtac aaggctcaca tgcaaaagct
    15901 tctggcgatc acgcgtaaca ccttcgttga aaccactcgg cagcccattt acagcgttct
    15961 gttgtgggcg gccgccttct gggtcggatt cgttagcccg gctctggccg gattcacgct
    16021 cgaagtcggc aacgacaaga aggtcatgat cgacgtgagc ctggccacgc tgctgctcta
    16081 cggcctgttg gccagcgtct tcagcgccag cagcgtgatc actcgcgaaa tcgaaagcca
    16141 cacagtattg accgttgtct ccaagccggt cagccgccca ctgtttttgc tcggaaagta
    16201 cctgggcgtg accggcgccg tactgctggg ctattacttc ctctgcctcg tgctgctgat
    16261 ggccgcccgc cacggcacca tggaaacggc cgctgagaag ttcgaccaac cggtgctggt
    16321 gttcggtctc tcggcgctgg cgatcgccct gatcgtgtcg gccttcggca attacgtcta
    16381 tggctggcac ttcactagca cgttgatggc gtgggcggtg ccgctggtca gcctggcgct
    16441 gttgctggta ctgttcattg atccggagtg gaagccgcag tcgccggcca aggactttgg
    16501 cgattttcaa ctcatctacg ccgagattag cgtcttctgc ggagtgctga ttctgacggc
    16561 cttcgccgtt gcgctcgcta cgcgcttcag cttggttgta acgctgatgc tttgtgcggc
    16621 gattttcctg cttggtctgc tgtcggacta ttattttggc gggcccgcta tggagggacg
    16681 cggtccgctt tacagcctgc tgtatggggc ggttccaaac ttccagtact tctggctggg
    16741 cgatccgatt acgcaacagc tcacgattcc cggccggcag gtggcggcgg tggccggata
    16801 tgccagcctc tacgcactgg cggtgctggc gctgggcgta agcctgttcc agacccgcga
    16861 ggtcggctga cgagcgctga caccgtgatg taggccgtct ctcaaccacc cgactgtcat
    16921 ccaaccggct gtagacccac gcggcccgct gcttccgtct ccatcgcggc gcgccggggc
    16981 accgccggtt tgtgcgcttc caaatgctcg ccaaagcgga ccatgcgggc actgttgaat
    17041 accacgatcg ctgacgagag cgtgtgcagg aacgcggcca tcatcggccc cacgaacttg
    17101 taaacgatgg ccacttccgc cacgaggatg aacgtgaccc cgaagatcag gttctgccag
    17161 atcacattgg tggtctcgcg cgagagattc accaggaatg gcagccgccg caggtcgttg
    17221 ctcatcaggg ccacgccggc gctgttcatc gcgatatcgc tgcccgccgc gcccatggcg
    17281 attcccaggt ttccggccgc cagtgccgga gcgtcgttta cgccgtcgcc caccaccgcc
    17341 acggtgtgcc cgcgcgcctg aagcgcgctg accagttcca gcttgtcctg cggcaggacc
    17401 tcggcctgca cgtccgagca gcccatttcc ttggctacgc gccgcgctac cgaccagcgg
    17461 tcgccggtga ccatgatcaa ctggcggatg cccaggtgcc gcaattcgtc cagcgattcg
    17521 tgggcctcgg gccgcgtgcg gtcctccagt ccgatccatc ccaggcattg cgtgccgcgc
    17581 actacgtaca gcgtgctcag gccttccggt tcgcggtact tttcatcgtc catggggccc
    17641 agatcaacgc cttgctcggc aacccacgtg cggcggccca ccgagacaat gtcgccattg
    17701 agccgccccg atacaccttt gcccggggct tccgcgaaat cagtagcctc ttcgagaggt
    17761 acccgggcac gctgtgcgac cgttgcaatg gcccgcgcca ccggatgccg gctgtactgc
    17821 tcgaccgaag ccgcggtgcg caacaaatcg gccgcatcca cgccgggcgc gggcatcagc
    17881 cgcgtcaccg ccaattcgcc ggtggtgagc gtgccggtct tgtcgaaaac cacagcggtg
    17941 agcttgcggg cccactccag gttgctcaca tctttgatgt aaatgcccag ccgggcggcg
    18001 gccgagaggg ccgctacgac ggcggtcggc gtcgccagaa tcagagcgca ggggcaggcg
    18061 atcacgagca gcgagatggt cacggtcatg tcgcgcgtga agaaccacac cacacccgcg
    18121 atcatcaatg tgaccggcgt gtaccaggcc gcgtattgat cgatcatccg catcagcggg
    18181 atcttggttc gttccgcgtc aagaatcagt tcttggacgc ggcccagcgt cgtgtcgtga
    18241 ccgaccttgg tgaccttgac ctgcagcgcg cccgtcagat tggtggtgcc gccgaagaca
    18301 tcatccccgg cgctcttttc caccggtaga gactcgccgg tgatgttcgc ttcgttgatg
    18361 gtcgacccgc cggagagaat cacgccgtcg gccgggatgt tgtcgccggg ccggatgtga
    18421 accacgtcgc cggctcgcag ggcgtgtgca tcgacgcgtt cctcggaacc atcgggcagg
    18481 atgcgatggg ccttggtagg cgtcagtcgc atcagtccct cgatggccgc gcgtgctccc
    18541 agagcggtgc gcgtctcaat caggttgctc agcagcagaa agaaggcgac gacgccggcc
    18601 gtctgatact cgccgatcgc catcgccgcg aggatcgcca gcgccaccag ttcgtccatg
    18661 tgcatgtggc cgccaaacag gcatttggcg gcgtgcaccc agagcggcac cgccagcagc
    18721 agcgccccca tggcggccag caggccggcg tagatatttc ggccgctgtc gtcgccgggc
    18781 gcgacttccc ccttgaacgc ccactgggcc acaaaggaca tcaggaccag catgccgccc
    18841 agcaatgttg caatcagctt gagcgtcgca cgtccggttt ccgatttcac ggacaagtga
    18901 tcatgcacca tgcgccaccc tggaagtcag cccagcgttt ctcccttggc gtagtgccag
    18961 ggacgacacg gttcggcaac ccgcgacgct ggcaagatca ggcactgtaa gcactaacga
    19021 ctctttgtca attcgagcgg agccgaggcg ccttgccccg ggcagcgccg cgtgtccaag
    19081 ccgagagcaa gcgcagggct tgtcaccccg gacaacgcgc ttggtcctcg cgggcatttc
    19141 gcgctcgttc tccctcgctc gcgcttcggg tcggatcgct gaccctcgtc gccacctggg
    19201 cgagtcgtcg tggcgagctg gcgcatcgcc tccgacggtt ggcgcggagc cggccgggtt
    19261 cttcacttga ttacccttac gagcggcagc gcgtccggtt cgcgacccca gcttgaattc
    19321 ttggctggct cgacgctgca atcggtgaag ctcacaacct tttgtggtaa ccgctttggg
    19381 tcaacatgct cgggtacagc cgcgcacgac ggcccatgca ttgatgtcga ccgtccccgc
    19441 cgcagccacc gtgcggatcg ccgcagggtt tctgtccggc acggccgtca acttgatgac
    19501 tggggcgacc tcgttggcgt caccgggccg cgcggcgcaa gggcgccgcg gaccgggtac
    19561 tagccataag agcatcgcgg gctgctgggg cacactaggc cgacggcgcc gcgccttcag
    19621 cggtgccctc ttccttcttg accttcttct tgcctgcgtg ggccttacgg tgcgcaactt
    19681 tcggcagccc cagcgggctg ctgtcttccg caaagcgctc ttcagttatg aggcgcgcga
    19741 ctcgctcggc tcggctgagc acgttacggt gccgctccag cgaactcttc ctgcgcagcg
    19801 atcgatcaac ggacatcaat ggactccgag atttcagccc ggcaatgcta ccgcgcagcg
    19861 gccggaagga caagtccgcc gattttccgt cagggcatcc gcggcccggt agcagccggg
    19921 gcgtgtcggc ccggaacccg ccacccgctt gatccgcgcg acgatgcgag cgttacgaca
    19981 gcccatcaag ttcggacgcc caagccggcc ctattcgtcc cagggcgtgt gcctggtgac
    20041 cagtgctcac agcggtaata ccgacgcggc aaggatcaag cacctcctga aaaacaggtc
    20101 agaagagtcc tgaaccacct cgcaggggcg acgaacgcat atgatcgcgc cggttgtcgc
    20161 gaacgccttt cagcgagcca tggcccttgg ttccgaaagc cagacaaaat catgaaccag
    20221 cgtattcagt ttcagaatgt cgcccctgag gcgctacagg cgatttacgg actggagaag
    20281 tacctgcgaa aatccggcct cgagcgggcg ctgctcgacc tggtcaagct gcgggcctcc
    20341 cagctcaacg gctgcgcata ttgcgtggac atgcacagca aggacctgcg tgccgccgga
    20401 gagagcgagg agcgcctctt cgctctgccg gtttggcggg agtcgcccta ctacaccgag
    20461 cgcgagcggg ccgcactggc ctgggccgaa gcgcttacca acatccagga cggccacgcc
    20521 ccggatgacc tctacgcctt ggtccggcag cagttcagcg agaaggaact cgtcgattta
    20581 acaatggcca ttgtcgcgat caacgggtgg aatcgcctgg cgatcgcatt ccgcgtgccg
    20641 gccggaacgt atcagccggc gcatcacgca gtaaagacgt gaaaatgtcc cgccgcgtca
    20701 gctactttcg agcaggtgcg cggcgcgcaa ttgattaagt aacaaaaaga agtaaatcaa
    20761 cccgcggtat ggcttgcagg acgccaggat ttgttggact ccgtcgtagt tgggacgttt
    20821 ttccaagtgc aaccaacttt gcagtttgct ctggctgccg acatcgtcag ccgggaaggt
    20881 gtcccagcgt cccaggccgc gcagcagcac gtactccgcc gtccaacgcc cgatacctgt
    20941 aaggttgagc aacgccttga acgctacggc atcctctttc gttgccaatt cctcgaggtt
    21001 caactccccg cgcacgacgg cccgagcgag ccagcgtatg gtttcgacct tgcgcccgct
    21061 gaaaccaagc tgccggatcc gcgcgcgtcg ctggcccgcc aaatcctccg gccgcgggaa
    21121 cgcgtgctgg tcaccaactg ccggaccgaa tgtggcagac agccggttca gtagatgaat
    21181 ccccacaatc agcgagagct gttgacaggc gatcccattg agcacggctt cgaaaacgga
    21241 aggaaaacgc ggcggcttga ggccgataaa gggcgccacg agaggcgcaa agtccttatg
    21301 ttctccaacg cgccggtgaa actcgcgcaa atccgcccgc agccccaaca cgcgctccag
    21361 cagcggcacc agcacttcac ggtcgcgcgc gccgatccga cgctcggacg tgcagacccg
    21421 caggcggggc gcgtcgcctc cggctacttg ctcgacggca acctcgaccg gccgcccccg
    21481 aaccggcacg acgcgccggt aagtctgccc gtcccagcga tcgatgaggt tgatcggcac
    21541 gcgccgcaac gcccacacgg tcagatccaa tcggaatggc gcgaccggtc gcaggaaaaa
    21601 acccgccatc cactgatttt agaaccgatt tgcactacac cagcacgtgc gatgcttatt
    21661 gcaaggcctc ccaacgcgca catgcctcaa gaggcggacg tacaacgcgg aacctgtcgc
    21721 gggtacagcc acccgcagtt ggctacggca gtgtgttgcc ggcagccagc agggcgacct
    21781 ggcgcatgag gtcgacggtc cggtggggat cgtgccgggc gaagagcttg cccgacagac
    21841 gcgagatgcc cagcatttcc ggcgcggcgc ccgtgctcgt gtccaactgc aggcttgcgg
    21901 attccaagtc gatttcgagg ccgtcgcccg accgcttggc cggatacgcg aagccggtcc
    21961 ccgtgtacga aacggtcccc tcccgcgtcg ccacgacata gcggatcgta gccgtcgtcg
    22021 tgctcgaatt ggaaaacgtc ttacccgggt acggctgcca ataaacatgg atgtgcagga
    22081 ggcgttcgag cggaccggcc gtatcgagcg cggctgccgc gtctcgggca a
//
LOCUS       DAOSYS010000028        21871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1957_length_21871_cov_5.115374, whole genome shotgun sequence.
ACCESSION   DAOSYS010000028 DAOSYS010000000
VERSION     DAOSYS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21871
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_21871_cov_5.115374"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1745)
                     /gene="ptsP"
                     /locus_tag="PLU99_03040"
     CDS             complement(3..1745)
                     /gene="ptsP"
                     /locus_tag="PLU99_03040"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOO16063.1"
                     /translation="MITKKGISASPGVAIGPALVLDTEEYRIPRRTIHPSQVAKELRT
                     LDAALEASRQEVSELRTALSRKMGHQTSAIFEFHEKFIADSKLRNTVAAKIENDLCTA
                     AYAFSHELNRQQKAFRAVPDQYIKERVTDLFDIEKRVLRHILGRTREDIARLSEPVVI
                     VAHDITPSQAVQLDRQHILGFVLNVGGQTSHMAIISRALRVPTVVGLTDATAEISGGE
                     TVIVDGTHGIVIAEPDQATLERYQALQQEYERFETRLRGLRDEPAITRDGQHISLLAN
                     IELPQEVEAALEAGAEGIGLYRTEFLFLGSDRMPTEEQQYEAFSSAVRAARGKPVTLR
                     TLDLGADKLATSMGVQHDHNPVLGLRSLRYCLLNLDLFKTHLRAMLRASVEGDVRIMF
                     PMIATLMELRQAKATLADVMEDLEEESIPFRRNTPVGIMIETPAAALVADALAQEVSF
                     LSIGTNDLTQYTLAVDRGNERVAHLYSPHNPAMLKLLRMIMRAGQRAGVEVSLCGEMA
                     GSAIYTQLLLGLGLRKLSMAPKDVPEVKQLIRSTTLADCQRIARKVMQFDADRQVLNF
                     LRDEARKVLPPEAF"
     gene            complement(1841..2110)
                     /locus_tag="PLU99_03045"
     CDS             complement(1841..2110)
                     /locus_tag="PLU99_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOO16064.1"
                     /translation="MVVEREVVIPNKEGLHFRPIMQFVDTAQRFAADVTVKCEGREAD
                     GRSPMELLMLVATHGTRVRLVADGNDAEAALEALSALIESGFGET"
     gene            complement(2201..2680)
                     /locus_tag="PLU99_03050"
     CDS             complement(2201..2680)
                     /locus_tag="PLU99_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO16065.1"
                     /translation="MKLLELIQPQSIIADLRATDRNGAIRELVQCLADSNLLDSQAVD
                     SIVKSIITRERTRGTTGFGKGVAVPHAKIEGLERVVAAVGRSAHGIDFSSLDGEPVYA
                     IFLIASPAEQPEEHLRAMDLVFRHLQQEKFRRFLRQSDEPEKIYDLLKEADEKMLVA"
     gene            complement(2705..3034)
                     /gene="raiA"
                     /locus_tag="PLU99_03055"
     CDS             complement(2705..3034)
                     /gene="raiA"
                     /locus_tag="PLU99_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOO16066.1"
                     /translation="MQVNVSGRHMGVSEALKEYCTEKAGKLTRYYDRIQSIDVVLDGK
                     NGVHTAEIIVHTDSTDPFVASETHEDVYAALDLLLDKIERQLTRHKERVRNRKHPPHA
                     SQENIQG"
     gene            complement(3335..4339)
                     /locus_tag="PLU99_03060"
     CDS             complement(3335..4339)
                     /locus_tag="PLU99_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOO16067.1"
                     /translation="MAKVTIEDISRFTGLSRGTVSRALNDRPDISVQTRQRVLEACRS
                     LNYSPSHAARCLAMGRAFAVAVLVDDLQAVFAATFLRGVLARARAARYAVFVAELGQD
                     EDRQLEAVEGAVGERIDCVLVSSPLSAGVTPVLMERLSGRPAIACLPLDGLNCDLLMP
                     DQVESGRLLAQHLLDDVGGPVLYVHGLDRPGATERLRGFEQCLQERGLDPVAHRFDMP
                     QERPLPDALENELVERLKGTRAVGASDDLLALELLNYCWRAGRRPGRDIAVMGQGNER
                     CAARISPPLTSTDWCGEEIGRRALEILLARVEKLRLDAPVSNYVAPVLCRRASTQLPS
                     "
     gene            complement(4554..7592)
                     /locus_tag="PLU99_03065"
     CDS             complement(4554..7592)
                     /locus_tag="PLU99_03065"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO16068.1"
                     /translation="MHSNRRLVVFPAAVALALLLLPLPGCAPGVSDGDDKGGTGKVFN
                     NPPVAKATADVTRGIVPLKVRFDSSRSTDSGGYIVSRVWDFGDGATATEITPTHVYNE
                     AGQFSVTLTVTDNQGATDTDSSITIFATQAPVAIIQADNYTAEFAPATISFDGSQSYD
                     PDGSVAGYLWSFGDGATASAAKVQHTFSRPGNFRVRLTVTDNFGVKSYAERVVVVGIG
                     KPVVSFRVPDETVTNIALAREAPLWISATYEVEPGTPYMISAGLDRDRDPCDAQAILY
                     DARTGDLLHHLLGAELPVRTAAFSPDGILALLAGDDGMLRLYDANFGEPLVSIDSGAD
                     SVNCLAFAPDGNRYVYGCNDGGLVIRHTQTGEIVRTLQAAPNSAVLSVAFSSAAEAIA
                     SGSADGNAVLWNTAGVQIMVLNEAIGGHSAAVNAVAFSPTDYAYLATGSADRTAKLWQ
                     IEPLQLLLTCTGHTDEVTCVAFSPGGGLLATGSLDQRVALWNAKTRAATPMAVFESFD
                     AGVTSLAFSPDGTGLLVGCANGSVHIIDLATQLTIRTLEPCTSAISSACYNAAGSMIL
                     LGVAAQNGIPLDTLNHEGADLNVRVPAALDLTSLTDAELSNACGEAGCAPYYLWVQLD
                     TDITEPVRAYARATVQVLESPAAEISDEAPVVPLVENQAVIVAAATPTRQVFSLPDLE
                     PGDQLKLSLATMPGYGRIYDTIGASGSGSSGARYSVLVLDGNRDLFVWYQNPGPAWDA
                     ADTPYWCEDRVSFSAADRLIVERDGPYYLVLDGCTRENCGTEYVAPSVVVEVVPGAAQ
                     PGESARQQRIYLNFNTAPDVVPINNEPVAMYPLDVAELNAGWGAGEKDDIVQKVHNKL
                     SDIFAGYDVVLSEDPAALTQPYTTVYFCGGNTTYLCWADCLDPRNQRRDGTAYVFANA
                     IKAKFLADPELTTTDGFAAAFANIAAQQIGLLVGLRNTIAGLGPGDVMDPAMLRSAAA
                     ITGPRAFINGSVSLNAGEQGNGGIGMQNARNLLLEVIGPG"
     gene            7742..8389
                     /locus_tag="PLU99_03070"
     CDS             7742..8389
                     /locus_tag="PLU99_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOO16069.1"
                     /translation="MPRPFSGSVGRRAPALFVLGIAALPLLLGGCPLNSTTQPDVYPG
                     GSQNANNTGLSSGSVSDPVDHTEARDTLERRFPGCGKPPEAEAWRDRVWQLLNSERMR
                     HGLEPLARNDVLDAQAEQYACEMIYYDFFAHVNPVTHSDLAERAAEFEYEYWMIGENL
                     AAGQTSPEQVVADWMASPGHRENMLGETYTEVGVGLRQGGDYGLYWVLEFGDPLD"
     gene            9000..9422
                     /gene="arfB"
                     /locus_tag="PLU99_03075"
     CDS             9000..9422
                     /gene="arfB"
                     /locus_tag="PLU99_03075"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOO16070.1"
                     /translation="MSRCRFHISDLTPWIEFDFERSAGPGGQNVNKVSTRAVLLFDFA
                     ACPLLSPPDKARIRQRLRHRLTRDARLRVVSQGERSQVRNRAAAEERLLELLASALYV
                     APRRRPTAPTAGSRERRLRRKRQRSETKRRRTRPAEDE"
     gene            complement(9456..10439)
                     /locus_tag="PLU99_03080"
     CDS             complement(9456..10439)
                     /locus_tag="PLU99_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO16071.1"
                     /translation="MNHVERFRAVMTFQPVDRWPRWEWAMWWDKTIARWHTEGLPREL
                     NHLQVFDIAQYFGLDPYQQFWFSPTDPNLANHVAGLVADMDDYRRIRPHIFPSHAAAI
                     AGMAPWAQRQRNGDVVVWCTIEGFFWFLRTLLGFSSLMLAFYRRPELLHAINNDLLEY
                     NLALLERVFGVCVPTFVTIAEDMSYNQGPMISEQMFASFFAPYYRRLVPRLQERGIPL
                     FVDTDGDVTLLVPWLQDLGVTGVLPLERQAGVDALRLRAQFPRLCMIGHFDKMTMSRG
                     EPAMRAEFERLMPLLKTGGFIPSVDHQTPPDVSLEQYRCYLRLLEEYTRGS"
     gene            10937..11845
                     /locus_tag="PLU99_03085"
     CDS             10937..11845
                     /locus_tag="PLU99_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16072.1"
                     /translation="MIRALVLGAFTLSLAGIGLAQQTGSQAYPEMGQYSGQYNPYGDQ
                     YTSSSQQGWQNVPYSGQYSPYYGQRQYTTQAMPRPSWDYGQMSTYEGAGTQWQSDQPT
                     WSIQRPYGLAGHGTWSQTGQPGWMSGQQAPWQYGQGTWQGAQQMPWQQYGHPGAYGTW
                     TPGQTAGFYGQQPGVGGMMSGQGQRLRILGSIEQHIPAQQFSNELNPNDVLVNVRTAS
                     GQEQLVDLGPNAENANFVPQPGSRIMVSGHVVFLNGRPVLIAQRAHEIGPGMFQQAGF
                     GERGGRFPAAGMWRGGTWRSGDQSGY"
     gene            12088..13044
                     /locus_tag="PLU99_03090"
     CDS             12088..13044
                     /locus_tag="PLU99_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOO16073.1"
                     /translation="MRSFDSLSEREVLALAIGLEEEDARIFADFADGLRQDYPASAEI
                     FDRMQQEEANHRRRLFDVYRERFGEHIPLVRREDVRGFVRRRPVWLARPLRLETVRKQ
                     AVVLEAEVRNFYLRAAKRVTDVGIRKLLNDLADEERVHEALAERLRQETLTADARSTE
                     EEARRRLFVLQIVQPGLAGLMDGSVSTLAPLFAAAFATRDSWDAFLVGLAASVGAGIS
                     MGFAEALSDDGSLTGRGHPWARGLVCGVMTTLGGVGHTLPYLIQNFHVATTVAVAVVA
                     LELAVISWVRHRYMDTPFMSAAFQVVVGGVLVFLTGILIGSS"
     gene            complement(13058..13372)
                     /locus_tag="PLU99_03095"
     CDS             complement(13058..13372)
                     /locus_tag="PLU99_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOO16074.1"
                     /translation="MKQTQNQPLEVVEPIGKRVLIRKDEDKKTTKGGIQLPGNIEIPT
                     ITGRIVAVSALVQRDEDYPISQYDKVLFNPKNAIPVDFEGDNRLFVVPIEDIVAVFRR
                     GG"
     gene            13443..>14038
                     /gene="larB"
                     /locus_tag="PLU99_03100"
     CDS             13443..>14038
                     /gene="larB"
                     /locus_tag="PLU99_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOO16075.1"
                     /translation="MEPQALKNLLESLAAGEVQVATALGRLRKWPFENLGFACVDHHR
                     GLRCGQPEVIFGEGKTPAQVETIFARLAAQGNNVLATRVSAAAAGPVLERYPAAEYHE
                     AARMLTLRQQPPRLTTGVVGLVCAGTSDLPVAEEARITLELMDQRVATYYDVGVAGLH
                     RLLAHAEALQAARVLIVVAGMEGALPSVVAGLVAAPVIA"
     assembly_gap    14039..14048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14049..14257
                     /locus_tag="PLU99_03105"
     CDS             <14049..14257
                     /locus_tag="PLU99_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HOO16076.1"
                     /translation="GASFGGLAALLGMLNTCAPGVSVVNIDNGFGAACQAALINRLGE
                     HETEQAASNSDPTHAAEKKDEHGA"
     gene            14241..15176
                     /locus_tag="PLU99_03110"
     CDS             14241..15176
                     /locus_tag="PLU99_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOO16077.1"
                     /translation="MSTGHSPPQIITELPYLAPRPPGMHKGQAGKVAIIAGCRGMSGA
                     AVLSGLGALRGGAGLVRVCTAESVQPVVATTEPCLMTAALPENAEHQVDGQRALDAFD
                     LTWPDVVAIGPGLGQSGGAAAFVRLLLESLKAPLVLDADALNNLSHSKTPWWTSRSGQ
                     QTIVTPHPGEMARLRRGAGLAETKGDDDETRLRIAHEYACLSGAVVVLKGFRTVVCTA
                     DRAYFNTSGDPGMATGGMGDVLTGLVAALLGQGIQAGGQFGAFEAACLGVYVHGAAAE
                     ACARQIGGFGYLAREVADAIPAALARASRGPMGFK"
     gene            15405..15698
                     /locus_tag="PLU99_03115"
     CDS             15405..15698
                     /locus_tag="PLU99_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HOO16078.1"
                     /translation="MPKFMCIHTMPPSSMTFQQVQELAERVRRDPVVEGHRSAGNLSE
                     GRIVCILESPTKQDLVNFFRNNNMPFDSISEVEFEGEGGVVTQIAAPAMAGQP"
     gene            15922..18369
                     /locus_tag="PLU99_03120"
     CDS             15922..18369
                     /locus_tag="PLU99_03120"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOO16079.1"
                     /translation="MACLSREQLEVLARGAADCAEVQELSRHVDECPACRRAVEALRS
                     SGATEVECDSPTILVGTGAQNEDGSDLDALAALTDSVIDCSSLLATAFPGYEIVKELH
                     SGAQGTVYQAIQKLTKRQVAIKVVREGPLASRAEKLRFEREIKILAQLRHPNIVTIHD
                     SGEAGGHAYYVMDYIPGQSLDKWSRSEPSIRAKLLLFAKLADAVAAAHQQGITHRDLK
                     PGNVQVDENGEPHVLDFGLAKTAGALEVTQVTRTGQFMGSLPWASPEQAEAVPGKVDI
                     RTDVYSLGVMLYEMLTGQLPYNMFGNMRDVLDRIINADPRRPSAVCGQIDHELETIVL
                     KCLQKPSERRYQSAGELADDVRRYLAGEPIQARGDSLFCLAMTRGRRTLRRHSLLAML
                     GAFVLITYAWCSLERYVYYWIPAHEQFLRVLAWFAAGAGSSDFEHVRVIGLTDRTDIA
                     TLARQANLTNVDPANLRSLRCMHGALMERLADSGLKALVWDINFERPSEFDESLARGI
                     RAVRSAGRDVLLTTRAWHIDSEPTFSPAVLKEAKWAAATGDMSAERPWSLDLTVERPE
                     NVPVPCLALAAVNAAFRPGAEFHLQVDGETCSLASRFWNTQGKRPLYGYYVQLTGRWP
                     LTEDDPDFGLLRNDVVGHYYITIPPDDVLARCTIEYQDVFTADQEQLRRWLGQRIVIM
                     GDLRTGVDRHPYPDGREVSGVYSHAAAIEAMSRYSAVRTPTENEAWVIISVAVLAGGL
                     VALFLHRSLAKLGFLTLAMIVLAGLFAVEAYRDFGYLANPTVPILAALTSAAFVTVTR
                     RRALNGSGAIQIEEKSQ"
     gene            18366..18953
                     /locus_tag="PLU99_03125"
     CDS             18366..18953
                     /locus_tag="PLU99_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16080.1"
                     /translation="MTEEASSVPAQLVEITSIVLGQQYVVPPNSVLKLTHQYPNGVRC
                     VFLAGGNPGESVEIHAVFTNSRPELAVDHGWSYLAVLPPAGSPPEPPPSGWGRASVTR
                     VSATANGTRLFVQEGDGIHRAVLLAGNSNPPGVVGVILNQDESNVHNLATANTYYEFL
                     PGQNGFNDPIPVDPDSEVGELVKYVEAMAVLAGVG"
     gene            19256..19564
                     /locus_tag="PLU99_03130"
     CDS             19256..19564
                     /locus_tag="PLU99_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO16081.1"
                     /translation="MPTRHRRDHERESVREPGCSPFSEQDWLLLAKTLDLSARQVEVA
                     KLLCEGFTYGGMAARLNISVNTVRMHMRALFAKLGTRDQVGVVVRLVVTQRRLARPQS
                     "
     assembly_gap    19643..19742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20177..>21871
                     /locus_tag="PLU99_03135"
     CDS             20177..>21871
                     /locus_tag="PLU99_03135"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOO16082.1"
                     /translation="MIRKGIMYTAGMLVLACTGTTVPVRGDAAAGPPMTRLEEPTGAC
                     CALGGVCWDTCTAAACWTSHAGIWQGPGSLCAAVDCYGACCIAGACYECDQALCLTLY
                     SGVWQGGGTSCDIAPCSPATGACCFSAGACEELPESTCESAGGAFIGLTIPCEPSPCT
                     TDYCTAGSAVCEEHIARIQIGRVDNASGCERYADYTHLGTCVVAGEQYLITVTNGLPF
                     EGDVCSVWIDWNGDHIFEDVAPEWIGDIVGGGASPPYRHPFTVPAGTLPGPKRVRIRI
                     DCNNPDPNPCGIRAYGEVEDYTLNVIPPAGACCVPSGYCEQLSEPDCIAAGGTVWLYD
                     APCQPTPCPQPEMLTLVADRNCYRAGDAVTVEVWMNNIAEGITGGQFFLSWDASKLAL
                     VDAVPGDHAESDPLNPFEMEIRRRISEGELDYAVGVHEPGAHPDPDGDDGASGHGTRR
                     MAVLTFAALVDICDQDGLLVWRAHSPPSRLSDTLGHPVTPELVDAHVADHVAPTITRP
                     ADVEIGCDEARTPDHTGWASAHDNCDYAPLIEWSDTEYLTGCGGTGTIIRHWTATDR"
BASE COUNT         3928 a         6723 c         7128 g         3982 t
ORIGIN      
        1 catcagaaag cctccggcgg tagaactttg cgcgcttcat cgcgcaggaa attaagaacc
       61 tggcgatcgg cgtcgaactg catcaccttg cgggcgatgc gctgacagtc cgcgagggtc
      121 gtggaccgga tcaactgttt gacttccggc acgtccttgg gggccatcga gagcttgcgc
      181 aagcccagcc ccagcagtag ctgcgtatag atggccgagc cggccatttc accgcacaag
      241 ctgacctcca ccccggcccg ctgcccggcg cgcataatca tccgcagcag cttcagcatg
      301 gctgggttgt gcggcgagta gaggtgcgcc actcgttcat tgccccggtc gaccgccagc
      361 gtgtattgcg tcaggtcgtt cgtgccgata ctgaggaaac tgacctcctg agccaatgcg
      421 tccgccacca gcgcggccgc gggcgtttcg atcatgattc cgacgggtgt gttgcgccgg
      481 aaggggattg actcttcctc gaggtcctcc atgacgtcgg ccagggtcgc cttggcctgc
      541 cgcaattcca tgagcgtggc gatcatcgga aacatgatgc gcacatcgcc ttcaaccgaa
      601 gcccgcagca tggcccgcag gtgcgttttg aacaggtcga gattcaacag gcagtaacgc
      661 agcgaacgca ggcccagcac cgggttatgg tcgtgctgca cccccatcga agtcgccagc
      721 ttgtccgcac ccaagtccag agtacgcagc gtgaccggct tgccgcgcgc ggcgcgcacc
      781 gcgctgctga aagcctcgta ctgttgttcc tcggtcggca tgcggtcgct tccgaggaac
      841 aagaactcgg tacggtacag gccgatgcct tcggcccccg cttcgagggc ggcctcaacc
      901 tcctgcggca gctcgatgtt cgccagcagc gaaatgtgct gcccgtcgcg cgtgatggcc
      961 ggttcgtcgc gcagcccgcg cagccgagtt tcgaaccgct cgtactcttg ctgtagggcc
     1021 tgataacgct cgagcgtggc ctggtccggc tcagctatca caatgccgtg cgtcccgtcg
     1081 acgatgaccg tctcgccgcc ggaaatctcg gccgtggcat cggtcaagcc gaccacggtc
     1141 ggcacgcgca gggcgcgcga gattatggcc atgtgcgacg tctgaccgcc gacgttcaag
     1201 acgaagccca ggatgtgctg acggtcgagc tgcacggctt gcgagggcgt gatgtcgtgg
     1261 gcgacgatta cgaccggctc gctgagccgc gcgatgtcct cgcgcgttcg ccccaggatg
     1321 tgccgtagca cgcgtttttc gatatcaaac aggtcggtga cccgctcctt gatgtactga
     1381 tcaggcaccg cccggaaggc cttctgctgc cggttcagct cgtggctgaa ggcgtaggcc
     1441 gccgtgcaga ggtcgttttc gatcttggcg gccaccgtgt tgcgcagctt ggaatcggcg
     1501 atgaatttct cgtgaaactc gaaaatggcc gaggtttggt ggcccatctt gcgggagagg
     1561 gcggtacgca gctcgctcac ttcctggcgc gaggcttcca gcgcagcatc cagcgtgcgc
     1621 agctccttgg ccacttgtga agggtgaatc gtgcggcgtg ggatacggta ctcttccgta
     1681 tccagaacca aagctggacc aatcgccacg ccgggcgacg cgctaattcc ttttttcgtg
     1741 atcattgccg cggacaccgc ggcgttcgcc gaccgcctcc cagccacgcg gtcgggttat
     1801 gccgtcagcc tcagttgctt ggtttcgttt tcaaatttac tcaggtctcg ccgaaccccg
     1861 actcgatcag ggccgacaag gcctccagcg ccgcttcggc gtcgttaccg tcggctacca
     1921 gccgcacccg cgttccgtgg gtcgccacca gcatcagcag ctccatcggg ctgcggccgt
     1981 ccgcttcacg tccctcgcac ttcaccgtca cgtcggccgc gaagcgctgc gcggtgtcca
     2041 cgaactgcat aatcggccga aaatgcagcc cctctttgtt cgggatcacg acctcacgct
     2101 cgacgaccac gttcaagaca tccatccatg gctgcgccac cggctcgtgg cgcaggcgtc
     2161 ccgcctatgt atccaccggc gagacgccgg tgcgacatct ttacgccacc aacatcttct
     2221 cgtctgcttc cttgagcaga tcgtagattt tctccggctc gtccgactgc ctcaagaagc
     2281 gccggaattt ctcctgctgt aagtgccgaa acaccaggtc catggcacgc aggtgctctt
     2341 ccggttgctc ggccgggctg gcgatcagaa atatcgcgta gaccggctcg ccgtcgagcg
     2401 aggagaagtc tatcccgtgg gcgctgcggc caaccgccgc gacgacgcgt tccagaccct
     2461 cgatcttggc gtgcggcacc gccacgccct tgccgaaccc ggtcgtgccg cgcgtgcgct
     2521 cgcgggtaat gatggatttc acgatcgaat ccaccgcctg cgagtcgagc agattggaat
     2581 cggccaaaca ctgcactagt tcgcggatcg ccccattgcg gtccgtggcc cgcaggtcgg
     2641 cgatgatcga ctggggttga atcaactcaa gcaatttcat atgcgtgttc gcctcggcgg
     2701 gccctcagcc ttgaatgttc tcctgggatg cgtgcggtgg atgctttcgg tttcgaacgc
     2761 gctccttgtg ccgcgtcagt tgccgctcga tcttatccag aagcaaatcg agtgccgcgt
     2821 atacatcctc gtgggtttca ctggccacga atggatccgt cgaatcggtg tgcacaatga
     2881 tctcggccgt gtgcacgccg ttcttgccgt ccaggactac gtcaatcgat tggatgcggt
     2941 cgtagtagcg cgtcagcttg ccggcttttt cggtacagta ttccttcagg gcctcactga
     3001 cacccatgtg tcggcccgac acgttaacct gcacttaatt ctccttttgc tgccgcaggc
     3061 aaccgcggtc ccccgaattc cgaccgccgc tgccgggccc gggcgcgtcg gccggcgtcc
     3121 gcgtgctgtg cttggcgcgc aaaaggacgg cccgagctgg cgcggagccg cgtacgttgg
     3181 ccgatgcttt tgcccatagc acaagcgaaa tgtatccgat gagcggcgca agcgtcaagg
     3241 cgggcgcacc cttttcctgg gttgcccggc ggacgaattc tgcttccccc ggacccgccg
     3301 gcgcccccgc cgggcccgat caaacgtgtc ggattcagct cggcagttgc gtggatgccc
     3361 gcctgcacag gaccggagcc acgtaattcg ataccggagc atccagccgc agcttctcta
     3421 cccgggccag caatatctcc agggcccgcc gcccgatttc ctccccgcac caatcggtgc
     3481 tcgtcagcgg cgggctgatc cgggccgcac aacgctcatt cccctggccc attacggcga
     3541 tgtcccggcc tgggcggcgg cctgcccgcc agcagtagtt gagcagttcc agggccagca
     3601 agtcgtcgct ggcgcccacg gcccgcgtcc ccttgagtcg ctccaccagc tcgttttcaa
     3661 gtgcgtcagg cagtggacgc tcttgcggca tgtcgaaacg atgagccacc gggtccaaac
     3721 cccgctcctg caggcattgc tcgaagccgc gcagcctttc cgtggccccg ggccggtcca
     3781 agccgtgaac ataaagcacc ggcccgccaa cgtcatccag caggtgctgg gccagcaacc
     3841 ggccggattc aacctggtcg ggcatgagca ggtcacaatt gagcccgtcc aacggcaggc
     3901 acgcgattgc cggccgcccg gacaagcgct ccatgagcac cggtgttacg ccggcgctga
     3961 gcggcgagga gaccagcaca caatcgatgc gctcgcccac cgcgccctcc accgcttcga
     4021 gctgccggtc ctcatcctgc cccaactcgg ccacaaaaac cgcgtagcga gccgcgcggg
     4081 cacgcgccag aaccccacgc agaaaagtgg ctgcgaagac ggcctgcaag tcatcgacca
     4141 gcacggctac cgcgaaagcc cgccccatgg ccaggcatcg ggcggcgtgg ctgggcgaat
     4201 aattcaggct gcgacaggcc tccagcacgc gctgccgcgt ctgaacgctg atgtcagggc
     4261 gatcgttcag cgcgcgcgaa accgtaccac gtgagagccc cgtgaaccga ctgatatctt
     4321 caatggtcac cttggccatg actcgcccca tgacctgccc cccgtcgcaa cgggggactc
     4381 aggcggccgt cggacctgca acacccacag acacgcgcgg attgtaccgc tttccgacgc
     4441 gctccgaaag gggcaatttg ccgtttccgt gcccctgttg agcaaatata tcgacttcgc
     4501 cgggcgggcg aatcaggggg cggaatcgga gctaaacccg gtgcgcgcct gcgtcaaccg
     4561 gggccgatga cctccagaag gaggttacgg gcattctgca tgccgattcc cccgttaccc
     4621 tgctcgccgg cgttcaggct tacggaacca ttaatgaacg cgcgcggccc agtgatggcg
     4681 gccgcgctgc ggagcatggc cgggtccata acgtcgccgg ggcctaagcc cgcgatcgtg
     4741 ttccgcaatc cgaccagtag cccgatttgc tgggccgcta tgttggcgaa tgccgctgca
     4801 aaaccgtctg tcgtggtcag ctccgggtcc gccaggaact tggccttgat cgcgttggcg
     4861 aacacgtagg cagtcccatc gcgccgctga ttgcgggggt ccaggcaatc cgcccagcac
     4921 agataagtcg tgttgccgcc gcagaaatac acagtcgtat acggctgcgt gagtgccgcg
     4981 gggtcctcgc tgaggacgac gtcgtacccg gcaaaaatgt cgctgagctt gttgtggact
     5041 ttctgaacga tgtcgtcctt ttcgcccgcc ccccagcccg cgttcagctc cgcgacatcc
     5101 aaggggtaca tcgcgaccgg ctcgttattg atcggcacca catcaggcgc tgtgttgaaa
     5161 ttcagataga tcctttgctg ccgcgcggat tcgccgggct gcgccgcgcc cgggaccact
     5221 tcgaccacca cactcggggc cacgtactcg gtgccgcagt tctcacgcgt gcagccgtcc
     5281 aacaccaggt agtacgggcc gtcccgctcg acaatcagcc tgtcggcggc cgagaaggaa
     5341 acgcgatctt cacaccagta cggcgtgtcg gccgcatccc aggccggacc cggattctgg
     5401 taccacacaa acaagtctcg gttcccgtcc aacaccagca cgctataccg agctccgctc
     5461 gaaccactgc cggacgcgcc gatggtatca taaatacggc catacccggg catggtcgcc
     5521 aagctcagct tcagttggtc gcccggctcc aagtccggca ggctgaacac ttgccgtgtc
     5581 ggcgtagctg cggcaacgat cacggcctgg ttttcgacca gcggcaccac aggcgcctcg
     5641 tcggaaatct cggcggccgg ggactccagg acttgaacgg tcgctctggc gtaagcgcgg
     5701 accggctcgg tgatgtccgt gtccaactgc acccacaggt agtacggggc acacccggct
     5761 tccccgcagg cgttggaaag ctccgcatct gtcagagagg tcaaatcgag ggccgccggc
     5821 acgcgcacat tcaaatcggc tccctcgtga ttaagcgtgt ccagcggaat cccattctgc
     5881 gccgcaacgc ccaggagaat catcgagccc gccgcgttgt agcaggccga gctgatcgcc
     5941 gaggtgcacg gttccagcgt ccgtatcgtg agctgcgttg ccaggtcgat tatgtgaacc
     6001 gaaccgttgg cacagccgac cagcagtccg gttccatcag gcgagaacgc gaggctcgtc
     6061 acgcccgcgt cgaatgactc gaaaaccgcc atgggcgtgg cagcacgtgt tttcgcattc
     6121 caaagcgcga cgcgctggtc cagactgccc gtggccagca gcccgccgcc gggcgaaaac
     6181 gccacgcagg tgacttcgtc ggtatgcccg gtacaggtca gcagcagttg cagcggctct
     6241 atttgccaga gcttggccgt gcgatctgcg ctgcccgttg ccaggtaggc atagtcggtc
     6301 ggggagaacg cgacggcgtt gaccgccgcg gagtgcccgc cgatcgcctc gttcaagacc
     6361 atgatctgca cgcccgccgt gttccagaga acggcattgc cgtcagcgct gccgctggca
     6421 atcgcctcgg ccgccgacga gaacgccacg cttagaacgg ccgagttcgg ggcggcctgc
     6481 aacgtgcgca ctatctcacc cgtctgcgtg tggcgtatca ccaacccgcc gtcgttgcac
     6541 ccgtaaacgt agcggttgcc gtccggagcg aacgccagac agttcacgct gtcagccccg
     6601 ctgtcgatgc tcacgagtgg ttcgccaaag ttggcgtcgt acagccggag catgccgtcg
     6661 tcaccggcca gcagggcgag aatgccgtcg ggtgagaaag cggccgtccg caccggcagc
     6721 tcggcgccga gcagatggtg cagcaggtcg ccggttcgcg cgtcatacaa gatggcctgc
     6781 gcgtcgcagg ggtcgcggtc acggtccagc ccggcggaga tcatgtaagg agtccccggt
     6841 tcgacctcgt aagtggccga gatccacagg ggtgcttccc gcgccagcgc gatgttggtc
     6901 actgtctcat cgggcacccg gaacgaaacc accggcttgc cgatcccgac caccaccacc
     6961 cgctcggcgt agctcttgac gccgaaattg tcagtgaccg tcagacgcac ccggaaattg
     7021 ccgggccgcg agaacgtgtg ctgcaccttc gctgctgacg cggtcgcccc gtcgccgaaa
     7081 ctccaaagat aaccggcgac gctgccgtcc ggatcgtagg actgcgaacc gtcgaacgag
     7141 atggtcgcgg gtgcaaactc ggccgtgtag ttgtcagctt gaatgatggc caccggcgcc
     7201 tgcgtggcga agatggtgat gctcgagtcc gtatcggtgg ccccctggtt gtcggtgacc
     7261 gtcagcgtga cgctgaattg cccggcctcg ttgtacacat gcgtcggcgt aatctcggtg
     7321 gccgttgccc cgtcgccaaa atcccacacg cgcgacacga tatagccgcc ggagtccgtg
     7381 ctgcgcgaag agtcgaagcg taccttcagc ggtacgatgc cgcgggtcac atcagccgtc
     7441 gctttagcca ccggcgggtt gttgaacact ttgccggtcc cgcccttgtc atcgccgtcg
     7501 gagacgccgg gcgcgcagcc cggaagaggc aggagaagca gcgccagggc gaccgccgct
     7561 gggaacacga cgaggcgacg attggaatgc atgcaacaaa ctcctcacta tcagcgcgtg
     7621 cgcacacgag gcgccttcgc tgctaagtat ggggggctcc gccagtcgta tcaaggtctt
     7681 gccccacttg ctatgatgga agtgattccg acttccttcg ccccggaggc gggagttgcc
     7741 tatgccgcgc cctttttctg gttccgtcgg ccgccgcgcg ccggccctct tcgtcctcgg
     7801 aattgcggcc cttccgctcc tgctgggcgg atgtccgctc aacagcacca cccagcctga
     7861 cgtctacccc ggcggcagcc agaatgcaaa caataccggc ctcagcagcg ggtccgtctc
     7921 tgacccggta gaccacacgg aagcgcggga cacgcttgaa aggcgttttc cgggttgcgg
     7981 caagcccccc gaggccgagg cctggcgcga tcgtgtttgg caactgctca acagcgaacg
     8041 catgcgccac ggacttgaac cgctggcgcg caacgacgtg ctcgacgcgc aggctgagca
     8101 gtacgcctgc gagatgatct actacgactt tttcgcccac gtgaaccccg tcacgcactc
     8161 agacttggcg gaacgggcgg ccgagttcga atacgagtac tggatgatcg gcgagaacct
     8221 ggcggccggg cagacttcgc ctgagcaggt cgtggctgac tggatggcca gccccggtca
     8281 ccgtgagaac atgctgggcg agacttatac tgaagtgggc gtgggattgc gccagggcgg
     8341 ggattatggg ctctactggg ttctggaatt cggcgatccc ctggactgac cctgaccgat
     8401 accgcccgcc ggacccaggc gccaacactc ggattcagct ttagttatcc ggcctactgt
     8461 cggcggcatt gtccggccgc ggttaccggg ccctgtcgac tatccgccac tgcaacccgc
     8521 cgcggcaccg ggggtaaccc ggtaataagg gtgcgctgcg acgcgctggt cgattggact
     8581 gacgcccgcg gcccacgaaa cagtcagcgc ttgatccgcc cgccccatgt gcatagaaac
     8641 agaaaaacca tgggatgaac catgataccg gcggcgctcg gcactggcgg cgcttggtat
     8701 aggcgagatc agcctgagga ggcgaaaggt gttttttgct tggacaccac aacgaatccg
     8761 catcgcaatt tatttcgcgg cggcatacct ggcgctgtgc tgatcggagc cgggggtgta
     8821 tcgccccgga atgtactaac ttggcggacg atcagagccg gggcgtgccg ccgcggaatg
     8881 ccactaacac gcctgaccgc gtagaatgat gaaatcccgg gctcgccacg gcgacgcgtg
     8941 ttcgccacgg cgaatcttcg caaagtagcc ccggaatacc gccagcgcgg tggcgctaaa
     9001 tgtcgcgatg ccggttccac atatccgatt tgacgccgtg gatcgagttc gatttcgaac
     9061 gttcggcggg ccccggcgga cagaatgtta acaaggtcag cacgcgcgcc gtcctcctgt
     9121 tcgacttcgc ggcctgcccg ctgctttcgc cgccggacaa ggcccgaatc cgccagcgcc
     9181 tgcgccaccg gctgacgcgc gatgcccgcc tgcgggtcgt ctcgcagggc gagcgctcgc
     9241 aggtccgaaa ccgggcggcc gctgaagaac gcctgctcga attgctcgct tcagccctgt
     9301 atgtcgcgcc gcgccgccgt ccgaccgccc caactgccgg ctcgcgcgaa cgccggctgc
     9361 gccgcaagcg gcagcgctcc gaaaccaagc ggcgccgcac tcgacccgct gaagatgaat
     9421 gatccgagcc ccggaaagcg cgtcgccgct tacgtttagc ttccgcgcgt gtactcctcc
     9481 agcaatcgca aatagcagcg atactgctcc agcgatacgt ccgggggcgt ctgatgatca
     9541 acgctgggaa tgaagccgcc ggtttttagc agcggcatca ggcgttcgaa ctcggcccgc
     9601 atggccggct cgccgcggct catcgtcatc ttgtcgaaat ggccgatcat gcacagcctg
     9661 ggaaactggg ctcgcaagcg cagcgcatcc acgccggcct ggcgctccag cggcaacacg
     9721 ccggttactc ccaggtcttg cagccacggc accagaagcg tcacgtcgcc gtcagtgtcc
     9781 acaaagagcg ggatgccgcg ctcctgcaag cgcggcacga gccggcggta gtacggcgcg
     9841 aaaaagctcg cgaacatctg ctccgaaatc atcgggccct ggttgtagct catatcctcg
     9901 gcgatcgtca cgaaggtcgg cacgcacacg ccgaacactc gctcgagcag ggccaggttg
     9961 tactccagca ggtcgttgtt gatcgcgtgc agcaattccg gccggcgata gaacgccaac
    10021 atgaggctcg aaaagcccag cagcgtgcgc aagaaccaga agaagccctc gatcgtgcac
    10081 cacacgacca cgtcgccgtt ccgctgccgc tgcgcccagg gcgccatgcc ggcgatggcc
    10141 gccgcgtgcg aggggaagat gtgcgggcga atgcgccggt agtcgtccat atccgcgacg
    10201 aggccggcca catggttcgc gaggttcggg tccgtcgggc tgaaccagaa ctgctggtac
    10261 ggatcgaggc cgaaatattg ggcgatgtcg aagacctgga ggtgattgag ctcgcgcggc
    10321 aggccctcgg tgtgccagcg cgcgatggtt ttgtcccacc acatggccca ttcccagcgc
    10381 ggccagcggt ccaccggctg gaaggtcatc acggcgcgga agcgctcaac gtggttcatg
    10441 tgctctcgaa ttccgtttcc gaaggcatca ccttattcac tgacagcggc tcgccgtcga
    10501 cggcccacag cacccttgag gggttgtgcc ggccgccggc ccgacagcct gctgactacg
    10561 cggagaataa ctcaacagag ctgagagtga aatacatcgc ccaatctcct gcgcctaccc
    10621 agccaccgtc tgacgagtcg attaatgacg aacagtcgta tcggattgga cgcgctggtt
    10681 ggataacgta atcacttgtg tagagtggca ctgcgccgcg tgttaatact tgtgtcgttg
    10741 gggacggcag accagtggca tgtgtattta ttcgggcggc gcgccgtgcg ccgcagccgc
    10801 gcgtatacaa gccggtcagg tgacggtgaa ttcagtgtgc ataaggccgt ccctgtattc
    10861 cgaccggtat aatgcgctta gatgacgagt gcacgcgggt gtctcgggac gttgtctaca
    10921 tgacagggag tacagcatga tcagagcatt ggtgttgggc gcctttaccc taagcttggc
    10981 cggtatcgga ctggcgcagc agacgggtag ccaggcgtat ccagaaatgg gccagtacag
    11041 cgggcaatac aacccctatg gagatcagta cacttcgagc agccagcagg gctggcagaa
    11101 tgtgccatac tcgggacagt atagcccgta ctacggtcaa cgccagtaca caacgcaggc
    11161 gatgccgcgg cccagttggg attatggaca gatgagcaca tatgagggag ccggcacgca
    11221 gtggcagagc gatcagccca cctggagcat tcagcgaccg tatggcctgg cgggccacgg
    11281 cacctggagc cagaccggcc agccgggctg gatgtccggc cagcaggctc cttggcaata
    11341 tggccagggc acttggcagg gcgcgcagca gatgccctgg cagcagtacg ggcacccggg
    11401 cgcgtacggc acctggaccc ccggtcagac ggccggcttc tacggtcagc agccgggagt
    11461 cggcggcatg atgagtggtc aagggcagcg cctgcggata ctgggttcaa ttgagcagca
    11521 cataccggcc cagcagttca gcaacgagct gaatccgaac gatgtgctgg tcaacgtgcg
    11581 caccgccagc gggcaggagc agttggtcga tcttgggcct aacgcggaaa acgcgaattt
    11641 cgtgccgcag ccgggcagcc ggatcatggt cagcggacac gtcgtgttcc ttaacggccg
    11701 gccggtgctg atcgcgcagc gggcgcacga gatcgggccg gggatgttcc aacaggccgg
    11761 ttttggggag cgcggcgggc ggttcccggc ggccggcatg tggcgcggcg gtacttggcg
    11821 cagcggggat cagtccgggt attgagccgg gcgcgccggc gcgcgaaatg cgtcgcggta
    11881 agcctgcgtg agcacaccgg cggaggcgac aacatcggaa gagcccggga cctgcgggta
    11941 ggttccgggc ttttcaattg cgcgcgcttc ttacacgcgg gcgctactta ctggactgct
    12001 ataatacaaa aatacaaatt taggtggcgg attgacgcgg tgggagggta cagaagtatc
    12061 cgcaggcagt aggaggatca cagcggcatg cgtagcttcg atagtttgag tgaacgcgaa
    12121 gtcctggctt tggcaatcgg cttggaggag gaggacgccc gcattttcgc cgacttcgcc
    12181 gacggtctgc ggcaggacta ccccgcttcg gcggagatct tcgaccgcat gcagcaggag
    12241 gaggccaacc accgccgccg cttgtttgat gtttaccgcg aacgcttcgg cgagcatatc
    12301 ccactcgtcc gccgcgagga cgtgcggggc ttcgtgcgcc gccgcccggt gtggctggcg
    12361 cggccgttgc gcctggagac cgtgcgcaag caggcggtcg tgctggaggc cgaggtccgc
    12421 aacttttact tgcgagcagc aaagcgcgtc acggatgtcg ggatccgcaa gctgctgaat
    12481 gacctggccg acgaagagcg cgtgcacgaa gcgctggccg agcggctgcg gcaggagacc
    12541 cttacggcgg acgcgcggag tacggaggaa gaggcccggc ggcggctatt cgtgttgcaa
    12601 atcgtccagc cgggtttggc cgggctgatg gatggctcgg tgtcgacact ggcgccgctg
    12661 tttgcggcgg cgttcgcgac gcgcgacagt tgggacgcgt ttttggtggg gctggcggcg
    12721 tcggtggggg ccggcatcag catgggattc gccgaagcgc tgagtgacga cggcagcctg
    12781 accggtcgtg ggcacccttg ggcgcgcggg ctggtatgcg gagtgatgac gacgttgggc
    12841 ggagtcgggc acaccctgcc gtacctgatt cagaatttcc acgtggcgac gacggtggcg
    12901 gtggcggtgg tggcgctgga gttggccgtc atttcatggg tgcggcatcg ctacatggac
    12961 acgcccttca tgtccgccgc gttccaagtg gtcgtgggcg gggtgctggt gtttttgacg
    13021 gggattttaa tcggcagctc gtgagctgcg gccgtcgcta gccgccgcgg cggaagaccg
    13081 caacgatgtc ttcgatgggc acgacgaaca gccggttgtc gccctcgaag tcgaccggga
    13141 tggcgttctt gggattaaaa agcaccttgt cgtactgcga gatggggtag tcctcgtcgc
    13201 gctgcacgag ggccgagacg gcgacgatgc gccccgtgat ggtggggatt tcgatgttgc
    13261 caggcaactg gatgccgccc ttggtggttt tcttgtcctc gtctttgcgg atcagcacgc
    13321 gcttgccgat cggctcgacg acttccagcg gctgattctg cgtctgtttc atgcgctcac
    13381 tcacgagtag actaccatca attgattcta tcggaaacgg ctcgtaaaag cgcggagtct
    13441 gagtggaacc tcaagcttta aaaaacctgc tcgaaagcct ggcggccggc gaggtccaag
    13501 tagcgacggc gctgggccgg ctgcggaagt ggccgtttga aaatctcggt ttcgcatgtg
    13561 tcgaccacca ccggggacta cgctgcggac agccggaggt catctttggg gagggcaaga
    13621 ccccggcgca ggtggagacg attttcgcgc ggctggcggc ccaaggaaac aacgtgctgg
    13681 cgacgcgggt cagcgccgcc gcggctgggc cggtgctgga gcgttacccg gcggccgaat
    13741 atcacgaagc ggcgcggatg ctgacgttgc ggcagcagcc gccgcggctg acgacgggcg
    13801 tggtgggcct ggtctgtgcc gggacaagcg atctgcccgt tgcggaggaa gcccggatta
    13861 cgctggagct gatggatcag cgggttgcaa cgtactacga cgtgggcgtg gcggggttgc
    13921 accggttgct ggcgcacgcg gaggcgctgc aggccgcgcg ggtgttgatc gtggtggcgg
    13981 ggatggaggg cgcgctgccg agcgtggtgg cggggctggt ggcggcgccg gtgatcgcnn
    14041 nnnnnnnnac ggggcgagtt ttgggggcct cgcggcgctg ctgggcatgc tgaatacgtg
    14101 cgcgccgggc gtttcggtcg tcaacatcga taatggcttt ggggcggcgt gccaggccgc
    14161 gctgatcaac cggctcggag agcacgaaac agaacaggcc gcttcaaact ccgatcccac
    14221 gcatgcggcg gagaaaaaag atgagcacgg ggcatagccc gccacagata attaccgagc
    14281 ttccgtacct ggcgccgcgt ccgccgggca tgcacaaagg ccaggccggc aaggtcgcga
    14341 tcattgccgg ctgccgcggc atgagcgggg cggcagtcct ttccgggctg ggagccttgc
    14401 gcggcggggc gggtctggtg cgggtttgca cggccgaatc cgttcagccg gttgtggcaa
    14461 cgaccgagcc gtgtctgatg acggcagccc taccggaaaa cgcggagcac caagtcgatg
    14521 gacaacgggc gctggatgct ttcgatttga cctggccgga cgtggtcgcc attgggccgg
    14581 gcctgggcca gtcgggcggc gcggcggcgt tcgtgcggct acttctggag agcctgaaag
    14641 cgccgctcgt gctggacgcc gacgcattga acaacctcag ccacagcaag acgccgtggt
    14701 ggactagccg gtcgggccag caaaccatcg tcactccaca cccgggcgag atggcgcggc
    14761 tacgcagggg ggcggggctg gccgaaacta agggggatga tgacgagacc cggttgcgaa
    14821 tagcgcatga gtatgcgtgc ctgagcgggg cagtagtggt tttaaagggt tttcgtacgg
    14881 tcgtctgcac ggctgatcgg gcgtacttca acaccagcgg cgaccccggg atggcgaccg
    14941 gcggcatggg cgatgtgctg accgggctcg tggccgcgtt gctggggcag ggcattcagg
    15001 cggggggcca gttcggtgca ttcgaagcgg cctgcctggg agtgtatgtt cacggggcgg
    15061 cggctgaggc ttgcgcccgg cagatcggcg ggttcggcta tctggcgcgc gaggtggcgg
    15121 atgcaattcc ggccgcgctg gcgcgcgctt cgcgcgggcc gatggggttc aagtaactgt
    15181 taactgtcgc ccttgctggc gctgcgggct cggatctggt ggatggggta cccttgctga
    15241 cgctgcgggc tcgtatcagg cggccgggcg gcgcctgcga aaaggcgtga cagcaatttt
    15301 agggcgtaga tagttcgaag ggcactgccg tccggcgttg acctacctat catccgaaat
    15361 ccgaccggtc agacaaaacg tgacaattct tgaggatttt caacatgcca aaattcatgt
    15421 gcattcatac gatgccgccc agctcaatga cgtttcagca ggtgcaggag ctggcggaac
    15481 gcgtacgacg cgatccggta gtcgagggtc accgctcggc cggcaacctc agtgagggca
    15541 ggattgtctg tatcctcgaa tccccgacca agcaggacct ggtcaacttc ttcagaaata
    15601 acaacatgcc cttcgacagc atcagtgagg tggaattcga gggcgagggc ggtgtggtga
    15661 cgcaaatcgc ggcccccgcc atggccgggc agccgtagcg gggagctgcc catagcggga
    15721 gactgtactc aaccatggcc cattgggcag ccgccgtcaa accgtgacag gcggtatgtc
    15781 ggcggctgcg gcgcccagcg tggtgctgca aagggaaggt tagaccggca attcgcaaat
    15841 ctatctgatg ggggctagtc gcctggtgcg cgaatcgggt atgctgcctg ccacctcgat
    15901 ctcgttcacc agggactgtc tatggcgtgc ctgtctcgcg aacaacttga agtgctggcg
    15961 cgcggtgcgg ctgactgtgc tgaagtccag gagctttcgc ggcacgtgga cgaatgtccg
    16021 gcctgccgca gggcggtcga ggcgttgcgc agcagcggcg ctacggaagt cgaatgcgat
    16081 tcgcccacaa ttctcgtggg gaccggcgca caaaacgaag acggctcaga cctggatgcg
    16141 ctcgcggcgc tgactgattc ggtaatcgat tgctcctcgc tgttggccac ggccttcccc
    16201 ggatatgaaa tcgtcaaaga gctgcattcc ggggctcagg gcaccgtcta ccaggcgatc
    16261 cagaagttga ccaaacgcca ggtcgccatc aaggtggtca gagaggggcc tttggccagc
    16321 cgcgccgaaa agctgcggtt cgagcgcgaa attaagattc tggcccagct ccggcatcca
    16381 aatatcgtga cgattcacga cagcggcgaa gcgggcggac acgcatacta cgtgatggat
    16441 tacatccccg ggcaatcgct cgacaagtgg tcgcgcagcg agccttctat ccgtgccaaa
    16501 ctgctgctgt tcgccaagtt ggccgacgcg gtggccgcgg cacatcaaca ggggatcacg
    16561 catcgtgacc tgaagccggg caacgtccaa gtggatgaaa acggcgaacc gcacgtcctt
    16621 gatttcggtc tggcgaagac ggccggcgct ctggaagtca cgcaggtgac gcgcaccggg
    16681 cagttcatgg gcagcctgcc ctgggcgagc ccggagcagg ccgaagcggt tccggggaaa
    16741 gtagacatcc gcaccgacgt gtactcgctc ggggtcatgc tgtacgaaat gctgacgggc
    16801 cagcttccgt acaacatgtt tggcaacatg cgggacgtgc tggaccgaat catcaatgcc
    16861 gacccgcggc ggccgagcgc agtttgcggg cagatcgatc acgagttgga gacgattgtt
    16921 ctcaagtgtc tgcagaagcc gagtgagcgc cgctatcaga gtgccggaga gttggcggac
    16981 gacgttcgcc gctacctggc gggcgagccg attcaggccc gcggggatag tctgttttgt
    17041 ctggcgatga cgcgcgggcg gcgcacgctg cggcggcatt cattgctcgc catgctgggg
    17101 gcgttcgtac tcataacgta cgcgtggtgt tcgctcgaac gctatgttta ctactggatt
    17161 ccagcgcatg agcagttctt acgggtgctg gcctggtttg ccgccggtgc cgggagttca
    17221 gacttcgagc acgtgcgcgt catagggctt acggatcgga ccgacattgc cactctggcg
    17281 cggcaggcaa atctgaccaa tgtggacccc gcaaatctcc gtagcctgcg ctgcatgcat
    17341 ggggcgctga tggagcggct ggcggatagc ggcctgaagg ccttggtgtg ggatatcaat
    17401 tttgagcggc cgtcagaatt cgatgagtcg ctggcgcgcg gcatacgtgc ggtacgcagt
    17461 gcggggcgcg atgtcttgct taccaccagg gcttggcaca tagactccga gccgaccttc
    17521 agcccagccg tacttaaaga agcgaagtgg gcggccgcaa ccggggatat gtccgccgaa
    17581 cgcccctggt cactcgatct tactgtagaa cgtcctgaga acgtgcccgt gccctgcctg
    17641 gcgctggccg cggtcaacgc ggcgttccgg ccgggggcag aatttcacct ccaggttgat
    17701 ggcgagacgt gcagcctggc gtcgcgcttt tggaatacgc agggcaagcg cccgctctat
    17761 gggtactacg tgcaactcac cgggcgttgg ccgctgacgg aagacgaccc ggatttcggc
    17821 ttgctgcgga atgacgtggt tgggcactac tatatcacga tcccaccgga tgacgtcctg
    17881 gcgcgctgca cgatcgaata ccaggatgtt tttactgctg atcaggagca gctaaggcgc
    17941 tggctaggtc agcggatcgt gattatgggc gatttgcgca caggcgtgga ccggcacccg
    18001 tacccagacg ggagggaggt gagcggagtc tacagccacg ccgcggcgat tgaagcgatg
    18061 agccgctatt ccgctgtcag aaccccgacc gagaacgaag cttgggtgat tatcagcgtg
    18121 gcggtgctcg ccggcggtct tgttgcgctg ttcctgcaca gatcactggc caaactggga
    18181 tttttgacgc tggccatgat cgtgctggcc ggcctcttcg cggttgaggc gtaccgcgac
    18241 ttcggctatc tggcaaatcc aacggtgcca attctggcag cgttgacaag tgctgctttt
    18301 gttaccgtga cgcgccgccg ggcgttgaac ggatctgggg caattcaaat cgaggagaaa
    18361 tcacaatgac cgaagaagca agcagcgttc ccgcgcaact ggtcgagatc acaagcatcg
    18421 tgctgggtca gcagtacgtg gttccgccga attccgtact gaagttgacg caccagtacc
    18481 ccaacggagt gcggtgcgta ttcctggccg gaggcaatcc gggagagagc gtcgaaatcc
    18541 atgcggtgtt caccaatagc cggcccgaac tggcggtcga tcacgggtgg tcatacctgg
    18601 ccgtgcttcc cccggcaggg tcaccgcccg agccgccgcc cagcggttgg ggtcgcgcga
    18661 gcgtaacgcg tgtgtcggcc acggcgaacg ggacgcgtct cttcgtccag gagggcgacg
    18721 gcatccaccg tgccgttctc ctggccggga atagcaatcc gccgggtgtg gtgggtgtga
    18781 tccttaacca ggatgagagc aacgttcaca acctggcgac tgctaatacg tactacgagt
    18841 tccttcctgg gcagaacgga ttcaacgacc cgatcccggt cgatccggac agcgaagtgg
    18901 gtgagcttgt gaagtacgtc gaggccatgg cggtcttggc gggcgtaggc tgagatattg
    18961 tccggccgac ttttgcatga cgttgtccag acttttgcac gaccgtgtgt attgtcggcc
    19021 ggctgggatt aacggcgcca gttatcgggc cgaaagtaga caggcccgac tggctccggg
    19081 gctacaccgc gcgggggccc ttgcgcccgg accgcgaccg ctggtcgcgc gccgggcgta
    19141 gacctatcct tttatgcgta gacacgggtc gcggcaggga tgatattgat tacggcatct
    19201 gacggccgct cagcttgact tcaaagcacg cgcagaacta gtcggagggt ggtttatgcc
    19261 aacgcgccac cgtcgggatc atgagcgcga atcggtaaga gaaccgggct gtagcccgtt
    19321 cagtgaacag gattggttgc tgttggccaa aacgctggac ttgagtgcgc gccaagtgga
    19381 agttgccaag ctgctgtgcg aagggttcac ctacggggga atggcggcac ggctgaatat
    19441 ctcagtcaac actgttcgca tgcacatgcg cgctttgttt gccaagttgg gcacccgcga
    19501 ccaggtagga gtggttgtac gactggtggt gactcagcgc cggctggcca ggccgcaatc
    19561 gtgacgatta attttgcacc tatcagttta tgcgtagcgg gcctggggcg aaatcctgta
    19621 cgctcattgt catcaggagg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19741 nnttatgcgt agcgggcctg gggcgaaatc ctgtacgctc attgtcatca ggaggcctga
    19801 gcaatttcgt tgcgttgctc cgtgacgcgg agatccagga aaatctggta gccgatcata
    19861 ttcgcccgtt agtcatttaa ccgcggccga gtggcctccc agcgtctaac gcccggcggc
    19921 aatttcgtcc gggtgtctgt tggtggtggc tcgcgcatgc gcggcgggtt cgcggcacat
    19981 gagccgcgca acccacggta gcggcgccga gcgggagaag gagcgtgtcc acgacagcgc
    20041 gtccgaagcg cctagggccg gggccgagtt ctcaattgcg acagagaaca caccagcgag
    20101 cggcgggacc ggtgcgccgc gcgggtcgaa ggcacgcaag gtggagatgc agccgggtgg
    20161 gtgtggagga cggccgatga tccgcaaagg catcatgtac acagcaggta tgctggttct
    20221 ggcatgcaca ggtacaactg ttccggtcag aggagacgcc gctgccgggc cgcccatgac
    20281 gcgccttgag gaacccacgg gggcctgctg tgctttgggc ggcgtgtgct gggacacttg
    20341 cacggcggca gcctgctgga catcgcacgc gggcatctgg caggggccgg gcagtttgtg
    20401 cgcagcggtt gactgctatg gcgcgtgctg catcgccggc gcctgctatg agtgcgacca
    20461 ggcgctgtgc ctgaccctgt actccggcgt gtggcagggc ggcgggacga gctgtgacat
    20521 cgccccctgc tcgccggcga cgggcgcctg ttgcttcagc gcgggggcat gtgaagagct
    20581 tcctgaaagt acgtgcgaaa gcgcgggcgg cgctttcatc ggcttgacca tcccgtgcga
    20641 accctcgccc tgtacaacag attactgtac cgccggctcc gccgtttgcg aggagcatat
    20701 cgcacgcatc caaattggcc gagttgacaa cgccagcggc tgcgaacggt atgcggatta
    20761 cacccacctc gggacctgtg tcgtcgccgg ggagcagtac ctgataacgg tcacgaatgg
    20821 cctgcccttc gagggcgacg tatgcagcgt ctggattgac tggaacggtg accacatttt
    20881 tgaggatgtt gcgcccgagt ggatcggcga cattgtcggc gggggcgcct cgccgcctta
    20941 tcgccatcca ttcaccgtac cggcgggcac gctgcccggc ccgaagcgcg tgcgcatccg
    21001 tattgactgc aacaatccgg atcccaatcc ctgcggtatc cgggcttatg gcgaagttga
    21061 ggattacacg ctgaatgtca tcccgcccgc cggcgcctgc tgcgtgccga gcggctattg
    21121 cgagcagctt tcagagcccg actgcatcgc cgcggggggc actgtttggc tatacgacgc
    21181 gccgtgtcaa cccacgccct gtccgcagcc ggaaatgcta acgctggttg ccgaccggaa
    21241 ttgctaccgg gctggagatg cggtcaccgt cgaagtgtgg atgaataaca ttgcggaggg
    21301 gatcacgggt gggcagttct tcctctcatg ggacgcctcc aagctcgcgc tggtcgacgc
    21361 ggttccggga gaccacgccg aaagtgatcc cctcaacccg ttcgaaatgg aaattcgcag
    21421 gcgaatctca gaaggcgagc tcgattatgc ggtcggcgtg cacgaacccg gcgctcatcc
    21481 cgaccctgat ggcgacgacg gggcgagcgg ccacgggaca cgccgcatgg ccgtcctgac
    21541 gttcgcggcg ctggtggaca tttgtgacca ggacggcctg ctggtctggc gggcccactc
    21601 gccgccgtcg cgcctgtctg acacgctcgg gcatcccgtt acgcctgaat tggtggacgc
    21661 gcacgtggcg gatcatgtcg cgccgaccat aactcgcccc gcggacgtcg aaatcggctg
    21721 cgatgaggcg cgcactccgg accacaccgg ctgggcttcg gctcacgaca actgtgatta
    21781 cgcgcctttg atcgaatggt cggatacgga atacctgacc ggctgcggcg gcaccggcac
    21841 gatcatccgc cattggaccg ccaccgaccg c
//
LOCUS       DAOSYS010000029        21842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1963_length_21842_cov_5.540689, whole genome shotgun sequence.
ACCESSION   DAOSYS010000029 DAOSYS010000000
VERSION     DAOSYS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21842
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_21842_cov_5.540689"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLU99_03140"
     CDS             <1..739
                     /locus_tag="PLU99_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16083.1"
                     /translation="FHPPAVPVGTDPCYVVSQEYRNGAADGIAFIDDYETQTAANTSS
                     SGGAVTYTVTNLTEGRLDDATSSFSWSASDPFNGATQDGASDTGRGVVFDWNDTDRYY
                     EWAVVSGLRDFSAWKYVGVRGAQGTQHPYTLETDGLLTFTITLRDGAGNTSSINSGAY
                     GGGFGMPYERSGGWHNEMRRIRIRITDFLANGSNIDLTNIVAVRLNVGPSWGTSQGRI
                     VIDELMLDNDLPPAGGLLMDEFEKALH"
     gene            959..4045
                     /locus_tag="PLU99_03145"
     CDS             959..4045
                     /locus_tag="PLU99_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16084.1"
                     /translation="MKLFPVLLLTGVLLVGPGSARAFELADMNCDGAVNVFDIDPFVM
                     ALTDPVTYAAEFPACDYLLADVNHDGDVNVFDIDPFVTCLTNGGCESAPTPPTPAELA
                     GNPLPAYPYFEYVRAFNQNAPINVAVDPTLHPEIVGQTADVYVVEKKIPGEWLTDPSL
                     VDVTAGGAMSVTFTGGTIQDNTFQVTGPSELSAAAFQAVTGDFTGLGHGYDMVVDMNR
                     NGVLDGGDYIDGLSREAGLYVCHDTTTPGPLAVTEVIYNVGTIFGIPASLPGQNLYYP
                     TNIANMGVLPLLVISHGNGHNYQWYDHIGNHMASYGFVVMSHGNNTGPGSIMASLTTC
                     GHTDAFLSLLPSIAGGVLVGHVDATRIIWTGHSRGGDGVAIAFTRISTDHSYTPTHYS
                     VDNIVMVDSMLPTDHYGPSMSNPRYSNYHLWTAAGDADVSSSTFHLHERATRYRQSTV
                     VQGAGHGWFHDADSYDPWFTGPCPINESGTHLIQLGLMLPMYKHYAEGNVPGADYLWR
                     MYEHFHPASVPIGIDPCYVVAQEYRNGAEDGIAFIDDYETNFSLDLSSSGGAVSYSVT
                     NLAEGRLDDADGSYSWSASDPFNGCDQDASGDPYRGAAFDWNDTDRFYEWAIVPELQN
                     FAAWKYISLRGAQTTRHPYTTETGGLLTFSITLRDAAGNTSSINTGAYGGGFGMPYGR
                     SGGWHNEMRRVRIRIRDFLANGSGLDLSNIVAVRLDVGPSWGTSQGHIVIDEMMLDND
                     LPAFFLPLTMGPLELPPEFLPPHVPVSFDLEIAEGDDALVEGSALMYYRLDGGDWNST
                     PLQNVAGELWRGTLPPLLCSDRPEYYFVAAGETTGPVFVPPAGPAAPYASLVGNYISI
                     LADDFESDLGWTVYNAPGMTSGMWQRAIPAGGQYSPPEDYDGSGRCYVTDNRVNYDVD
                     LGPTQLISPVFDFSDADDPVIRFAEWMYCDDATGMYPSQQDYLDVHLSSDGGVTWVQV
                     AHIASHEDWALHDIHVADYIPLTATVQVRFTASDNPNNSNTEVAIDAVTVFDVQCD"
     gene            complement(4241..5539)
                     /locus_tag="PLU99_03150"
     CDS             complement(4241..5539)
                     /locus_tag="PLU99_03150"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOO16085.1"
                     /translation="MSKTILIRGGRVIDPSQQIDEVTDVVIAQGVIAALGRGAPTADE
                     VFNAAGCIVAPGLIDMHVHLREPGQEEKETIETGTAAAAAGGFTAVACMPNTEPALDS
                     DSEIEFVLRQAARVARTRVYPIGALTKGRAGKELAEIGLMVRAGAVAFSDDGDCITDS
                     AVCLRAMNYLSMFDRLFIQHCEDKSLTGRGCMNGGETATMLGLPGMPAMAEDLIVQRD
                     IMLASHTNVRYHVAHITTKGAVELVRRARAAGRRVTAEVCPHHLLLTEESCTTYDPNF
                     KMNPPLRSKADREACLQGVQDGTIDCIVSDHAPHGPQDKEHEFQDVPFGIIGLETSLA
                     LAIRALIEPKIIDWPQLIRAMSTRPAELLGVSGGTLKVGAPADVTVIDPQREWTVDPE
                     RMRSKARNTPFGGWKLRGAAVATLVDGHFRLRNDELLPPV"
     gene            5658..6557
                     /locus_tag="PLU99_03155"
     CDS             5658..6557
                     /locus_tag="PLU99_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO16086.1"
                     /translation="MKTHGELLRRRFESGILVLDGATGTELERHGLVTSIPLWSAAGL
                     FECPELVEQIHRNYAAAGADIVIANTFRTNARTLEAAGMLESGAELNACAVWLARQAV
                     SSEGVLVAGSVGPVADCYCPELTPEEVILEEEHAQMAEWLQAAGADLLWIETIGTVRE
                     ARAAAAAAYGRGLPFAISFILAENGTLLGGEPLEAAVAAVEKFRPLAIGLNCIPPRGL
                     TALLPELRQLTRRPVAAYGHINNPTPIRGWSYSQAVSPAEYGDYARQWLDLGASIVGG
                     CCGTTPEHIAAVRAVVSACGAAT"
     gene            complement(6579..8240)
                     /locus_tag="PLU99_03160"
     CDS             complement(6579..8240)
                     /locus_tag="PLU99_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16087.1"
                     /translation="MIALILRLFDCFSWLLRGLGVDYPQFRAILQTKLMLDVRRPRGA
                     FAQRSNKNRNMFALTLVSLAFMGVFISPLVAKVQSPLVSLTFIHAVIMVMLAMSLLGD
                     YSTVLLDTTDNHVLLPRPVSRRTVLFARLAHIFTYLLLITASLGAATFVVGTIVRGGA
                     FLPAFALSLTLSVGLIVGVVSLAYLVGMRLIPEERLRDLLTYAQIVMLVVVVAGYQLV
                     PRLVGSAVKAKLDLAAQNWIYFCPPTWMAGLVDLALGNRGAAQVVLSIEALLAPVLLL
                     LIVAVLAPTFQPAALEGDRTGSAKRAAAGAIRQRWPAVLARLVARNPAERGAFDLVWR
                     LAGRDRQYKLRVYPGLAFAFVFALIWMLTGAERGQTVTLGGLPQTDRHLILLYCASLA
                     APAALLHLRFSSQFDAAWIYSALPLYRPGEVLLGAFKAVMVRLMLPTFAVLAAIALVV
                     WGWRVLPDVLLASVVLVLICLIEALLVGRHLPFSESAGAMESSGRTLRSMLLMPMVGV
                     FGLAHYAIRSYPFVVLGAIPIVLLLAFACARLYQRTTWSAIPRAS"
     gene            complement(8237..9022)
                     /locus_tag="PLU99_03165"
     CDS             complement(8237..9022)
                     /locus_tag="PLU99_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16088.1"
                     /translation="MRPIEITDLTKSFGEKVAVRGVTLSVPAGQVIGYIGPNGAGKTT
                     TVKILCGMLDGFGGRVSVCGVDVAQQPLEVKRRIGYIPESAALYAMLTPLEYLQFIGT
                     LYGLERRETERRARELLGLFGLADELDNRMSTFSKGMRQKVLIVSGLIHNPEVIFMDE
                     PLSGLDANSAVIVKEIITRLAQRGKTVFYCSHVMDVVERVCDRIVIINDGRIIADGTF
                     EELQAMNKAASLERIFTQLTSAGGQADVAGRFVSALEGGGTGR"
     gene            9171..10355
                     /locus_tag="PLU99_03170"
     CDS             9171..10355
                     /locus_tag="PLU99_03170"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO16089.1"
                     /translation="MREIVWLMSLIGLSWQIACKAPQPVAPPVAPREPLAELCPPAID
                     PMLAVNTLKPAPPGFLDCRLATPEYAIPPYAKFLAGVRICLDPGHGGDAHKRGYKRGP
                     TGVREAEVDLRVAQYLRELLLRAGAEVRLTREGDCDVGLSERAAIANEWPADLFISLH
                     HNANDDPQVNCTTVWYHAGVDHRPASLDLARYLCFSLQDGLAHPQIADVPLRSDQLMY
                     DIGFAVLRETNMPGALTESSYHTNPAEEQRLRDPEYNLREAWALFLGLAKYAAAGLPR
                     ARLLEPANGILLLPAASTTDLAEQTAPPQIVFELDDGLRSRRDRGWERQMILTDSIIV
                     RLDGQPVPHTFSNEGYRLCVELPRDLPPGEHEIRVDFQNMNKNSVLNPLFRLVVVSAP
                     GD"
     regulatory      10472..10737
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10918..13347
                     /locus_tag="PLU99_03175"
     CDS             10918..13347
                     /locus_tag="PLU99_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO16090.1"
                     /translation="MQRQRLGGSGGTVWRLVCGLLVGVCAWSVRAADPWADAIVSYDA
                     GSDALPGYDQVATVLGPPERYTGELGGWPAAVTPFNPAWCSDEILSLGTGGHITVRFD
                     EPLTDDPAHPFGVDLLVFTNSFFADVQYPDGVVGGFFGESPFNVSLSADGSTFVPLPG
                     SYSTGLFPTLGYLDAAPQDPSAGTILTDFTRPVDPTITLDDLMGQSYAELLARYNGSG
                     GGIPIDLAAGGLAEVYYVRIDVPPNAGPIAFDAFATVPEPSSLISFGVLLAAFACSRG
                     GPCRAGLRGSVLRSARTCGVRGTRRAFRGRAPRFCAILLLLIFAIATANAASWTHYAG
                     DSARVAVAPAGPLQLDEPAWIAPPPVDTEFVYDSSPVVYGGRVFVKGRRFSGWTPVSA
                     RVLAYDSKTGEELWIQDVDLDYYDDWSSPAIDAANGTVIVGAGYRLHALDTITGEIRW
                     QCQLERRIVNASPAVSFNLISGGPPADRVFISDYSGAGSQARLYAINVSPYEATINPF
                     QPGEIAWSASLPGASGATPAYYDGYVYAATRTGVIYAFDAYNGEPHWQTDVAAAGYPG
                     YAAFYGGVAIHDGYLYAASYSFYGSGNNSGLFKLHADSGEIAWVTPCERTDSIPVVTA
                     NGRIFLAGGLDWAGSGTKIQAFQDLGTTATLLWDTDVATGGDLVVGGWTHQPAFAGGL
                     LYAGTPSEYAFSPYTELFILNTAHSPAQPGFIVDYSTVAGGSPAIAGGWLYSIGAPGL
                     VAFARRITGDANCDGMINVFDIDPFVLALTDPATYAAAYPDCDLNHADANGDGEVNVF
                     DIDPFVSLLTG"
     gene            13478..14266
                     /locus_tag="PLU99_03180"
     CDS             13478..14266
                     /locus_tag="PLU99_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO16091.1"
                     /translation="MPEKSTTSPAFSLIELLVVVSVIALLCSLLLPTLGQARAQARNL
                     VCSAHLAQLGHAFHMYAVEYRGRAMPLVYSDKFPEVYWWGTDDPAGVDHTRGFIWPYL
                     PSDLRPESLFECPNQPWGSYDAQGEAKQVTSTYGYNGYYLCPPHAGYHETKRQPWKDI
                     DQLYWPDRLFTFGDTLIDMGGRPRNNTHLDPPFRYYGRRWMPNGSPTTAFRHNGRANI
                     LLADGHVDAFGLEGGSLQNAEYSIGSVGPTNDPHYVPDWREWSK"
     gene            complement(14377..15477)
                     /locus_tag="PLU99_03185"
     CDS             complement(14377..15477)
                     /locus_tag="PLU99_03185"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOO16092.1"
                     /translation="MKRTLSKWHETYVEQNGMKRTLSRVEMEAAFARRDKAYDGLFYV
                     CVRSTGIFCRPSCPARKPLPRNTDFLTSVRDALLAGYRPCKRCRPLDANGRPPQWVSD
                     LLYALARAPGERLSDDELRARGIEPARARRYFRSNYGMSFQAYCRHWRLGQALADLRR
                     GMQLSQTALKYGFESESGFRDAFRRTFGGPPGRRRMLDTLVSSRLESPVGYLEVAANS
                     EGICLLEFEDRRALPTQWKVLARRFGTPVVPGRNEHIERLADELARYFAGELREFTVP
                     LVIRGTPFQERVWRRLLQIPFGETLSYEGLARAVGCPGAQRAVGRANGDNRLAIVVPC
                     HRVVQKDGQLRGYGGGLWRKQFLLDLERRTRG"
     gene            15742..17025
                     /locus_tag="PLU99_03190"
     CDS             15742..17025
                     /locus_tag="PLU99_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HOO16093.1"
                     /translation="MKLTDSEKRMLAGEVPLAGSAAAVAKAMDYLVQFGDAFGAERFV
                     DVIYAHYPAEMSIYRGNVEDALEYAETGARVCIPTTSSTLACDLEQWRDYGCPPAIYE
                     LQQRVVPAHRKLGILGTYTCTPQLLGFVPPKGSYIITVESSAIIYYNSVLGARTNRGG
                     LFTRYAAVTGKYPLTGYLLDENRRGTHLFKLHLERDDLRENCDFSALGFHVGGIVGSE
                     VPVFDALPAGTTQDNLIALGAALATSGSVTLFHAPPHTTEARTVAEALHGGRPKETHT
                     VTRADLRAVFDKLTTIAPSGPVDFVTLGCPHYSLEQLRWVADWFVSRNARVHSNVRLW
                     VCTNRMTRRAAEWQGITQSIEAAGGRVICDTCPVESHMRISTCKEHGLPTPQIQAMVT
                     DSCKMARYVGDLIGCKTALRSRDQCLAAAAAGRLV"
     gene            17450..18736
                     /gene="lysA"
                     /locus_tag="PLU99_03195"
     CDS             17450..18736
                     /gene="lysA"
                     /locus_tag="PLU99_03195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOO16094.1"
                     /translation="MDCFRYQNGSLYCEDVAAAELAARFGTPLYVYSQNTFTNHYQRL
                     RQAFAAVDPLICFSIKACQNVHICRVLRELGSGFDVVSGGELFRALRAGADPQRIVFA
                     GVGKTERELNEALDAGIGLFNIESESELAELTRICRRRGVRPRAALRVNPDVDPHTHE
                     YTTTGKKETKFGVDLDRAVRVFHRFAPRSPVRLCGIHLHIGSPVFDVAAYRRSLERGI
                     ELIERLRSDGLEVDTIDIGGGYAADYQAEEAPRPEEYADAIVPLLKGRDLRVILEPGR
                     SIAANAGVLLTRVLHVKRSGGRRFLIVDASMNELIRPALYGAYHFIWPVAAGDRVPVS
                     RQAEQPFEGLDLSDVVGPVCESGDFLARQRMLPPVKRGDLLAVYSTGAYAMVMASQYN
                     SRPRAAEVLVNGGLARLIRRRETYEDLVAAEELESA"
     gene            complement(18816..20051)
                     /locus_tag="PLU99_03200"
     CDS             complement(18816..20051)
                     /locus_tag="PLU99_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16095.1"
                     /translation="MSTATAQPERMDFAADVESVVCRLRDSMADVIARVPGLTYRRPN
                     DLAADLGLATKLAWKVGRCVDVADPFAAAQFVPGPTGMRAFLRAAQRRGVPKPALDVA
                     RAAFEDFRKLVQTHGGTRKSFDMLAAGLAGTDQMRADLEHRRLAFEGNTYVWGVRART
                     IFRANIVHPSSDLETWDLATVRGFIDFRCMRPKVAWRIARPASVDRSHNVHSELSRGA
                     LDPRAADGVPLLIDFCSQPVPQFRPVIGLGGDVEFEFVESRVGNTGRMTCVTGELVRR
                     IEPRYRTELYPDLCVMYPVRTPAEVLIFDLLVHESIFGGGEPPTPELYSDLFGGGPGV
                     RYEPADRLPLHDPLVYLGNGPDVARTPDIPRYPEMLRYALEQTGWNGRELKLYRLRMQ
                     YPTMPTTLMLRKPLPRRPA"
     gene            complement(20399..20695)
                     /locus_tag="PLU99_03205"
     CDS             complement(20399..20695)
                     /locus_tag="PLU99_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16096.1"
                     /translation="MATMFQALGSPLEIPAVEGKIIKIDRLTQSMRGTVMGTKVLRVQ
                     SNSNDILPNMCCTTLNDLRWPDHQPQGERGQSVVIDVSGNDPLISVWVAYRYVD"
     gene            21421..>21842
                     /locus_tag="PLU99_03210"
     CDS             21421..>21842
                     /locus_tag="PLU99_03210"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOO16097.1"
                     /translation="MDQVEIVTRQADLEAYCRNWRRLGRLAFDTEFIRDETYESALCL
                     IQVAADGQVVLIDPTAPIDLASFWDLVTDPTVTTIVHAGKEDFEVCLRSTARTPCNVF
                     DVQIAAGFLGYGYPLSLTRLMADVLGQRISKGQTLTDWL"
BASE COUNT         3993 a         7353 c         6746 g         3750 t
ORIGIN      
        1 cttccatccg ccggctgtgc cggtcggcac tgacccctgc tatgtcgttt cgcaggaata
       61 ccgcaacggg gccgcggacg gaatcgcgtt tatcgacgac tatgaaacac agaccgcggc
      121 gaacaccagc agttccggtg gcgcagtgac gtacaccgtc accaacctga ctgaaggccg
      181 gctggacgac gctaccagca gcttctcctg gagtgcgagc gatccgttca acggcgccac
      241 gcaggacggc gccagcgaca cgggccgcgg cgtggtattc gactggaacg atacggacag
      301 atattacgaa tgggcggtcg tgtcgggtct gcgcgacttc tcggcctgga aatacgtcgg
      361 cgttcgcggc gcgcagggca cgcagcatcc gtatacgctc gagaccgacg gactcctgac
      421 gttcaccata accctgcgcg acggcgccgg taacaccagc tcgatcaata gcggcgctta
      481 cggcggcggc ttcggcatgc cgtacgaacg cagcggcggc tggcacaacg agatgcggcg
      541 cattcgcatc cgcatcacgg acttcctggc gaacggcagc aatattgacc tgaccaacat
      601 cgtggcggtg cggctgaacg tcggcccctc atggggcacg agccagggcc gaatcgtcat
      661 tgacgaattg atgctcgaca acgacctgcc gcccgcgggc gggctgttga tggacgagtt
      721 cgagaaagca ctccattaac ttggcgcggc ggtttgcggc cccaaggctt gccaagtaag
      781 ccgtaaaccc cgctgccgtc agcagttatg acgggaaaac ggccgggcgc cgcgcggcgc
      841 ccggctgctc tttttttcta tgagtctggg cggcggcgcg ttaaactcgg aggcggttcg
      901 ggctaattcc agccgcgtag cggaaagaag tttttggcac ttacaaagga ggcctgttgt
      961 gaagctcttc ccagtgctgt tgctgactgg cgtcttgttg gtaggtcccg gctcggcgcg
     1021 cgccttcgaa ctcgcggaca tgaattgcga tggcgcggta aacgtctttg acatcgatcc
     1081 gttcgtcatg gcactgaccg acccggtcac ttacgccgcc gagttcccgg cctgcgatta
     1141 cctgctggcc gatgtcaatc acgacggcga cgtgaacgtg ttcgacatcg acccgttcgt
     1201 cacgtgcctg accaacggcg ggtgtgagtc cgcgcccacc ccgccgaccc cggcggagct
     1261 ggccggcaat ccgctgccgg cctaccccta cttcgagtac gtacgggcct ttaaccagaa
     1321 cgccccgatc aacgttgcgg ttgacccaac actccacccg gagatcgtcg gccagaccgc
     1381 ggacgtatac gtcgtcgaaa agaagattcc gggcgagtgg ctgacggatc cgagcctggt
     1441 ggacgtcact gccggtggag cgatgagcgt cacgttcacc ggcgggacga ttcaggataa
     1501 caccttccag gtcaccggcc caagcgagct gagtgcggcc gccttccagg ccgtgaccgg
     1561 ggacttcacg gggctgggcc acggctacga catggtcgtg gacatgaacc gcaacggcgt
     1621 gctggacggc ggcgactata tcgacgggct gagccgcgaa gccggcctgt acgtgtgcca
     1681 tgacacgacg acgcccggac cgctggcggt gaccgaggtt atttacaacg tcggtacgat
     1741 tttcggcatc cccgccagct tgcccggcca gaatctttat tacccgacaa acatcgcaaa
     1801 catgggcgtg ttgccgctgc tggtgatcag ccacggaaac ggccataact atcagtggta
     1861 cgaccacatc ggcaatcaca tggcgtctta cggcttcgtc gtcatgtcgc acgggaacaa
     1921 cacggggccg ggttcgataa tggcctcgct gacgacctgc ggccacacgg acgcttttct
     1981 gtctctgctg cccagcattg ccggcggcgt gctggtagga cacgttgacg caacgcgaat
     2041 catctggacg ggccacagcc gcggcggaga cggcgtggcg atcgcgttca cgcgcatcag
     2101 caccgaccac agctatactc cgacgcacta cagcgtggac aacattgtca tggtggacag
     2161 catgctgccg accgaccact acgggccgag catgtcaaac cccaggtact cgaattacca
     2221 cctctggacc gccgcgggcg atgcggacgt ctcgtcctcg acgttccacc tgcacgagcg
     2281 tgccacgcgc taccggcaat cgaccgtggt tcagggtgcc gggcacggct ggttccacga
     2341 cgctgacagt tacgacccct ggttcacagg gccctgcccg atcaacgagt ccggcacgca
     2401 cctgatccaa ctgggcctga tgctgccgat gtacaagcac tacgcggagg gcaacgtgcc
     2461 gggcgcggac tacctttggc ggatgtacga gcacttccat cccgcctctg ttccaatcgg
     2521 catcgacccc tgttacgtcg ttgcgcagga gtatcgcaac ggcgccgagg acggcatcgc
     2581 cttcatcgat gattatgaaa ccaatttctc actcgacctc agcagctccg gcggcgcggt
     2641 gtcttacagc gtgacaaacc tggccgaagg gcgcctggac gacgccgacg gcagttatag
     2701 ttggagcgcc agcgacccgt tcaacggctg cgaccaggac gcctcgggcg acccgtaccg
     2761 cggcgccgca tttgattgga acgatacgga ccgcttctac gaatgggcca tcgtgcctga
     2821 gctgcagaac ttcgccgcct ggaagtatat ctccctccgc ggcgcgcaga ccacccgtca
     2881 cccctacacc accgaaaccg gcggcctctt gacattctcc atcacgctgc gcgacgcggc
     2941 cggcaatacc agttccatca ataccggcgc gtatggcggc ggcttcggca tgccttacgg
     3001 ccgcagcggc ggctggcaca atgaaatgcg ccgcgtgcgc attcgcatcc gggacttttt
     3061 ggccaacggc agcggcctcg atctgagcaa catcgtcgcc gtgcgcctgg acgtgggtcc
     3121 gtcctggggc acaagccagg gacacatcgt cattgacgag atgatgctgg acaacgacct
     3181 gccggctttt ttcctgccgc tgaccatggg accgctcgaa ctgccgcccg agttcctgcc
     3241 cccgcatgtg ccggtgtcgt ttgacctgga gattgctgag ggcgacgacg ccctggtaga
     3301 gggttccgcc ctgatgtact accgcctcga cggcggcgac tggaactcca cccccttgca
     3361 aaacgtggcc ggcgagttgt ggcgcggcac actgccccca ctgctgtgca gcgatcggcc
     3421 ggaatactac ttcgtggccg cgggagagac gaccggtcct gtcttcgttc cgcccgccgg
     3481 tccggctgct ccgtatgcct cgctggtcgg caattacatc agcattctcg cggacgactt
     3541 tgaaagcgat ctcggctgga ccgtgtacaa cgcccccggt atgacttccg gcatgtggca
     3601 gcgggccatc ccggccggcg ggcaatactc gccgccggag gactatgacg ggtccggccg
     3661 ctgctatgtc accgacaacc gcgtcaacta tgacgtagac ctggggccga cgcagttgat
     3721 ctcaccggtg tttgacttca gtgacgccga cgacccggtg attcgcttcg ccgagtggat
     3781 gtactgcgac gacgcgaccg gaatgtaccc ctcgcagcag gattacctgg acgtccacct
     3841 gtcgagcgat ggcggagtga cctgggtgca agtcgcgcat atagccagtc acgaggactg
     3901 ggcgctccat gacattcacg tagcggacta cattccgctg acggcgacgg tacaggtgcg
     3961 cttcacggcc agcgacaatc ccaataattc aaacaccgag gtcgcgatcg acgcggtgac
     4021 cgtgtttgac gtacaatgtg actaacagga catacaatgc aatgtgattg cggcgggtgt
     4081 gattgaggcg ggtgccatgc ccaagcggca agcgcgggca tggcactggc aaaggccatc
     4141 aactacgaag gccatgaact gctaagggca tacccgcgcc cgccatgcgg ggcttgcaca
     4201 ggccccctca acgtcagcca tcacgccact gctgccgctg ctaaacgggc ggcagcagtt
     4261 cgtcgtttct caggcgaaag tggccgtcca ccaatgtcgc aacggccgcg ccacggagtt
     4321 tccagcctcc aaacggcgtg ttgcgcgcct ttgaacgcat ccgctccgga tcgaccgtcc
     4381 actcgcgctg cggatcgatc acggtcacgt cggccggggc gccgactttc aacgtgccgc
     4441 cgctgacgcc cagcagttcg gccgggcggg tcgacatcgc ccgtatgagc tgcggccagt
     4501 ctatgatctt cggctcgatc agggcccgga tggccagagc gagcgaggtt tccaggccga
     4561 tgatgccgaa gggcacgtcc tggaactcgt gctccttgtc ctgaggtccg tgtggggcgt
     4621 ggtcgctgac gatgcagtcg atcgtgccgt cctgcacgcc ttgcaggcag gcctcgcggt
     4681 cggccttact gcgcagcggc gggttcatct tgaaattcgg gtcgtaagtc gtgcaggact
     4741 cctccgtcag cagcaagtgg tgcgggcaaa cttccgccgt cacgcggcgg ccggccgccc
     4801 gggcgcggcg aaccaactcc accgccccct tggtggtgat gtgcgccacg tggtagcgca
     4861 cgttcgtgtg ggaggccagc attatgtcgc gctgcacgat caggtcttcc gccatcgcgg
     4921 gcatgcccgg caggccaagc atggtcgccg tctcaccgcc gttcatacag ccgcggcccg
     4981 tcagcgactt gtcctcgcaa tgctggatga aaaggcgatc gaacatgctc aggtagttca
     5041 tggcacgcag gcaaacggct gaatcggtga tgcaatcgcc gtcgtcgctg aaggccaccg
     5101 ccccggcgcg caccatgagc ccgatttctg ccagctcttt gccggcccgg cccttggtga
     5161 gagcgccgat cgggtaaacg cgcgtgcgcg ccacacgcgc cgcctgacgc agaacaaact
     5221 ctatctccga atcagaatca agggccggct cggtgttcgg catgcaggcc accgccgtaa
     5281 agccgccggc cgccgcagcc gccgtgccag tttcgatcgt ctctttctct tcctggcccg
     5341 gctcccgcag gtgcacgtgc atatcgatca ggcccggcgc cacgatgcaa ccggccgcgt
     5401 taaaaacttc gtcagccgtc ggagcgccgc ggccgagggc ggcaatgacg ccctgggcga
     5461 tgacgacgtc cgtaacctcg tcgatctgct ggctgggatc gattacgcgt ccgccgcgaa
     5521 tcaaaatcgt cttgctcata caccacgcgc ctgtgtaggc cctgagttcc ggcccgttgg
     5581 tcgggctgcc cggcgagcta tcatactctt atggcggcac aggggtagcc ccgcacctgg
     5641 ggactgccgg agtgacgatg aagacccacg gcgaattatt gcggcgccgc ttcgagagcg
     5701 gcattctcgt tctggatggc gcgaccggga ccgagctgga acgtcacggc cttgtcacca
     5761 gcatcccgct ctggtcggct gcggggctgt ttgagtgtcc cgagctggtc gagcagattc
     5821 accgcaatta cgcggcggcc ggggccgata tcgtcatagc gaacaccttc cgcaccaacg
     5881 cccggactct ggaagcggcc ggcatgctgg agagcggggc ggagctgaac gcctgtgcgg
     5941 tgtggctggc ccggcaagcc gtgagtagtg agggcgtact cgtcgcgggc agcgtcggcc
     6001 cggttgcgga ttgctactgc cccgagctca cgccggagga agtaatcctc gaggaggagc
     6061 acgcgcagat ggccgagtgg ctgcaggccg ccggcgcaga tctgctgtgg atcgagacta
     6121 tcggtacggt tcgcgaggcc cgcgcggcgg cggcggccgc ttatggcagg ggtttgccgt
     6181 tcgcgatctc gttcatcctg gcggagaacg gcacgttgct gggcggcgag cctttggagg
     6241 ccgccgtggc cgcggtcgaa aagttcagac ccttggccat cggcctgaat tgcatcccgc
     6301 ccagaggatt gaccgccctg cttcctgaat tgcggcaact cacgcgccgc ccggtggcag
     6361 cctacggcca catcaataac cccacaccga ttcgtggctg gagctactcc caggccgtct
     6421 caccggcaga gtatggcgat tacgcccggc agtggctcga cttgggggct tcgatcgtcg
     6481 gcggctgctg cggaacgacg cccgaacaca tcgccgccgt gcgggccgtg gtcagcgcct
     6541 gcggcgcggc cacgtaaacg ccggcagtcg gcgtgcggtc agctcgcgcg cgggatggcg
     6601 ctccaggtcg ttcgctggta cagccgcgcg caagcaaatg ctaaaagcag gacgatcggg
     6661 attgcgccca gcaccacgaa cggataggag cggatcgcat agtgcgccag accaaataca
     6721 cccaccatcg gcatgaggag catgctgcgc agcgtacgcc cgctgctctc cattgccccc
     6781 gccgattctg aaaatggcag gtgccgcccc accaacaacg cttcaatcag gcaaatcagc
     6841 actaagacca ccgaggccag caatacgtcg ggcagcacgc gccagcccca aacgaccaac
     6901 gcgatggcgg ccagaaccgc aaaagtcggc agcatcaggc gaaccatcac tgccttgaat
     6961 gcacccagca gaacttcgcc cggccggtac agcggcagtg cagagtaaat ccacgccgcg
     7021 tcgaactggc tcgaaaagcg caggtgaagc aaagcggccg gggctgcgag cgacgcgcaa
     7081 tacaacagga tcaggtgccg gtccgtttgc ggcaggccgc ccaaagtgac cgtttggccc
     7141 cgctccgcgc ccgtcagcat ccagataagg gcgaagacga aggcgaacgc caaaccgggg
     7201 tagacgcgca acttgtactg gcggtcgcgg cccgccagac gccagaccag gtcaaaggcg
     7261 ccgcgctccg cagggtttct ggcaaccagg cgcgccagta ccgcgggcca gcgctggcgg
     7321 atggcgcccg ccgccgcacg cttcgcagaa ccagtccgat cgccctccag cgccgcgggc
     7381 tgaaacgtcg gcgccaggac ggcgacgatc agcaacagca acaccggcgc taacagcgcc
     7441 tcgattgaca gcaccacctg cgctgccccg cggttcccca gcgccaggtc caccaggccc
     7501 gccatccacg tcggcggaca gaaataaatc cagttctggg cggccaggtc gagtttggcc
     7561 ttaaccgccg agcctaccag gcgcggcacg agctggtagc ccgcgacaac gaccacaagc
     7621 atcacgatct gcgcgtaggt aagcaggtcg cgcagccgct cctctggaat cagccgcatc
     7681 cccaccagat aagccaggct aacgacgccc acgatcaggc ccacagacaa cgtcagcgac
     7741 aaggcgaaag ccggcaggaa cgcgccgccc cgcacgatcg tcccgacgac gaacgtcgcc
     7801 gcacccaggc tcgccgtaat gagcagcaga tacgtgaata tgtgggccag ccgggcaaag
     7861 agcaccgtgc gacggctgac cgggcgcggc agaagcacgt ggttgtcggt cgtgtccaac
     7921 aagacggttg aataatcccc caatagcgac atcgccagca tgaccatgat taccgcatgg
     7981 atgaacgtca gacttaccag cggcgattgc accttcgcca ccagcggtga gatgaagacg
     8041 cccatgaacg ccagggaaac cagcgtcagc gcgaacatgt tgcgattctt gttgctccgt
     8101 tgcgcgaatg cgccccgcgg ccgccgcacg tccagcatca acttcgtctg cagaatggcg
     8161 cggaactgcg gatagtccac gcccaaaccg cgcagcagcc acgaaaagca gtcgaacagc
     8221 cgcaaaatca gcgcaatcat cgccccgttc ccccgccctc cagcgcagat acgaagcgtc
     8281 cggccacgtc cgcctgcccg ccggcgctgg tgagctgcgt aaagatgcgc tccagcgagg
     8341 cggccttgtt catggcctgc aactcctcga acgtgccgtc ggcgatgatg cgcccgtcgt
     8401 tgatgatgac gatgcggtcg caaacccgct caaccacgtc catcacgtgc gagcagtaga
     8461 aaaccgtttt gccgcgctgc gccaggcgcg tgataatctc cttgacgatc acggccgagt
     8521 tcgcgtccag ccccgaaagc ggctcatcca taaaaatcac ttcggggttg tggatgagac
     8581 ccgagacgat cagcaccttc tgccgcatac ccttcgaaaa agtggacatg cggttgtcca
     8641 actcatccgc caggccgaag agcccgagca gctcgcgcgc ccggcgctcc gtttcccgtc
     8701 tttccagccc gtaaagcgtg cctatgaatt gcaggtattc cagcggggtg agcatggcgt
     8761 acaaggcggc ggattcagga atgtagccga tgcggcgctt cacctcgagc ggctgctggg
     8821 cgacgtcaac gccgcagacg gaaacccgcc cgccgaagcc gtccaacatg ccgcacaata
     8881 tcttcacggt ggtcgtcttg cccgcaccgt tcggcccgat gtagccgatg acctgcccgg
     8941 ccggcacact cagcgtgacg ccgcgcacgg ccactttttc cccgaaactc ttggtcaaat
     9001 ctgtgatttc gattggacgc atgccagcct tatgtagccc atgtcggccg ggtccgcaga
     9061 ttcgacgtat tgcggtcgcc gtgtcgcatc cgccaacccg acattcgcgg ttagtatacg
     9121 cgagcttcgg actcggatga gagcggcatg gcgtcgaaag aggtgagcgg atgcgagaaa
     9181 ttgtctggct gatgtcactg atagggctta gctggcaaat cgcctgcaag gcgccgcagc
     9241 ctgttgcgcc gccggtcgcg ccgcgcgagc cgctggccga gctttgcccg ccggccatcg
     9301 atccaatgct tgcggtgaac acgctcaagc cggcgccgcc gggattcctg gattgccgcc
     9361 tggccacccc ggaatacgcc attccgccgt acgcgaagtt cctggccggc gtgcgaatct
     9421 gccttgaccc gggccacggc ggcgatgccc ataagcgcgg ctacaagcgc ggaccaacgg
     9481 gcgtgcgcga ggccgaggtg gacctgcgcg tggcccagta cctgcgcgaa ctgctgctgc
     9541 gggccggcgc cgaggtgcgc ctcacgcgcg agggcgactg cgacgtcggc ctgtcggagc
     9601 gggccgccat cgcgaatgag tggcccgccg acctcttcat ctcgctgcac cacaatgcga
     9661 acgacgatcc gcaggtcaac tgcacgaccg tctggtacca cgccggcgtc gaccatcgcc
     9721 ccgcaagcct cgacctggca cgctacttgt gcttcagcct gcaagacggc ctggcccacc
     9781 cgcaaatcgc cgatgtgccg ctgcgcagcg accagcttat gtacgacatc ggcttcgccg
     9841 tgttgcgcga gacgaacatg cccggcgcct tgaccgaaag ctcctaccac acgaatccgg
     9901 ccgaggagca gcggctgcgc gacccggaat acaacctgcg cgaggcgtgg gcactgttcc
     9961 tcggtttggc gaaatacgcc gcggcgggat tgccgcgcgc tcgactactt gagcctgcta
    10021 atggcattct cctgctaccc gccgcgtcga ccacagactt ggccgagcag accgcgccgc
    10081 cgcagattgt ctttgagctg gacgacggcc tgcgctcgcg tcgcgaccgc ggctgggagc
    10141 ggcaaatgat cctgaccgac tcgatcatcg tacgcttgga cggccaaccg gtcccgcaca
    10201 cgttcagcaa cgaggggtat cgtctctgcg tagagctgcc gcgcgacctg ccgccgggcg
    10261 agcacgaaat ccgcgttgac tttcagaaca tgaataagaa ctccgttctg aatccgctct
    10321 tcagactggt cgtcgtttct gcgccgggcg attgacaacc ggcgcgggca cggtggctgc
    10381 gaacgcattt cgcccgcgcg aaattatccg atgtcacgac tcgggaacgc cggccgccgc
    10441 acccctattg acacgccccc cgccaaagcc taaaagacgg ccggtcactg gtgtccgccg
    10501 ctcgtggcgg tcggaagaat agggaatccg gtgcgaaacc ggaacggccc cgccgctgtg
    10561 accgggaacg actgctgcac cgtcattggc tcgccgtgcc gagctgagaa gacgcagccc
    10621 gtcggtgcga gtgcgaagtc caggacgccg cgtcccggat tgccgtggga tgatccatcc
    10681 cggctgctac ccgcgaaccc ggaagtcaga agacctgcca gtggaccgca gaagtcggat
    10741 tgcctcgtgg gtagggacag ccggcatgcc tctctcggat tttcgcatcc gctgttgaac
    10801 tgctgaccca gcgccgccgc gcgcagcgcc ggcgcctgcc agcattggcg cgaccgtccc
    10861 caaccccgaa cgcaatccaa gtacgccgcg cgggtgccgc ggcttaggag gagatgtatg
    10921 caacggcagc gtcttggagg cagcgggggc acagtctggc gcttggtctg tggactgtta
    10981 gtaggtgtat gtgcgtggag cgttcgggcc gccgatcctt gggcggatgc aatcgtctcg
    11041 tacgacgcgg gcagcgatgc gctgccgggc tacgaccagg tcgcgaccgt gctcggcccg
    11101 cccgagcgct atacgggcga actgggcggc tggccggccg ccgtcacccc cttcaatccc
    11161 gcctggtgta gcgacgaaat cctcagcctc ggaaccggcg gccacattac ggtgcgattc
    11221 gacgagccgc tgacggacga cccggctcat ccgttcggcg tcgacctgct cgtgttcacc
    11281 aacagcttct tcgccgatgt gcagtatccg gacggcgtgg tgggcgggtt cttcggcgaa
    11341 agcccattca acgtatcact gagcgccgac ggcagcacgt tcgtgccgct gcccggctca
    11401 tactcgaccg gattgttccc cacgctcggc tatctggacg cggccccgca agatccgtcg
    11461 gccggaacca tcctcaccga cttcacccgc cccgttgatc cgacgatcac gctggacgac
    11521 ctgatgggcc agtcgtatgc ggagttgctc gctcgctaca acggttcggg cggtggcatc
    11581 cccatcgacc ttgcggccgg tggcctggcg gaagtctact acgtgcgcat cgacgtgccg
    11641 ccgaacgccg gtcctatcgc cttcgacgca ttcgccactg tgccggaacc aagctcgcta
    11701 atcagctttg gcgttctgtt ggccgcattc gcctgtagca gaggcggtcc gtgtcgggcc
    11761 ggactgcggg gaagtgtgct ccggagcgcg cgcacgtgtg gtgttcgagg cacacggcgc
    11821 gcgtttcggg gccgcgcccc ccggttctgc gcgatcttgc ttctgctgat atttgcaatc
    11881 gcgacggcaa acgccgcgtc ctggacacac tacgccggtg acagtgcacg cgtcgccgtc
    11941 gcacccgcgg gcccactgca actggacgag cccgcctgga tcgctccgcc gccggtggac
    12001 acagagtttg tatacgacag ctcgccggta gtgtacggcg gacgcgtctt cgtcaaaggc
    12061 cgccgcttct ccggctggac cccggtcagc gcgcgtgtcc tggcgtacga cagcaagacg
    12121 ggcgaagaac tctggattca agatgtagac ctggactact acgacgactg gtccagcccg
    12181 gccattgatg ccgccaacgg caccgtcata gtcggcgccg gctaccgcct gcacgccctc
    12241 gacacgataa ctggcgagat tcgctggcaa tgccagctag agcggcgcat cgttaacgcc
    12301 tcgcctgcgg tcagcttcaa cctgatcagc ggcgggccgc cggctgaccg cgtcttcatc
    12361 agcgattatt ccggcgccgg cagccaggcc cgcctgtacg ccatcaacgt cagcccctat
    12421 gaagccacca tcaatccctt ccaacccggc gaaattgcct ggagcgccag cctgccgggc
    12481 gccagcggcg ccacacccgc ctattacgac ggctacgttt acgccgccac cagaaccggt
    12541 gtcatttacg ccttcgacgc ctacaacggc gagccgcact ggcaaaccga cgtcgccgct
    12601 gccggctatc ccggatatgc cgcgttctac ggcggtgtgg ccatccatga cggctatctc
    12661 tatgccgcca gctacagctt ctatggctcc ggcaacaaca gtggactatt caaactgcac
    12721 gccgacagcg gagaaatcgc ctgggttacg ccctgcgaac gcaccgattc cattcccgtc
    12781 gtcaccgcta acggccgcat cttcctcgcg ggcggcctcg attgggcggg ctcgggcacg
    12841 aaaatccagg ctttccagga cctcggcact acggccacgc tgctatggga tacggacgtc
    12901 gcgaccggtg gggatctggt cgtcggcggc tggacgcatc agccggcctt cgcgggcggc
    12961 ctgctttacg ccggcacgcc gagcgaatac gcgttcagcc cctacaccga gctgttcatc
    13021 ctgaacaccg cccacagtcc ggcccagccg gggttcatcg tggattacag cactgtcgca
    13081 ggcggcagtc ccgcaatcgc cggcggctgg ctgtattcca tcggcgcgcc cggcctggtc
    13141 gccttcgcgc gccgcataac cggcgacgca aattgcgacg gcatgatcaa tgtcttcgac
    13201 atcgatccgt tcgttctcgc cctcaccgac ccggctactt acgccgccgc gtaccccgac
    13261 tgcgacttga atcatgccga cgccaacggc gacggcgaag tgaatgtgtt tgacatcgat
    13321 cctttcgtca gtctgctgac cgggtgagcc aatgaccgcc cctcaatgta gcgtcggccc
    13381 cccgccagcg ttccaatgta gcgccggccc cccgtggccg acgtcagccg ccaaagaccc
    13441 accctgccca cgacagaagc gaacatctgc aaccccgatg cccgagaaga gcacaacatc
    13501 gccggccttc tccctgatcg agctgctggt cgtcgtcagc gtcatcgcgc tgctgtgctc
    13561 actgctgctg ccgacgctcg gccaggcccg cgcgcaggcc agaaatctgg tctgctccgc
    13621 gcacctggcc cagctcggcc acgccttcca tatgtacgcc gtcgagtacc gcggccgcgc
    13681 catgccgctg gtgtacagtg acaagtttcc ggaagtgtac tggtggggaa cggacgatcc
    13741 ggcgggcgtg gaccacacgc gcggcttcat atggccgtac ttgcccagcg acttgcgccc
    13801 ggagagcctt ttcgagtgcc cgaatcagcc ttggggcagt tacgacgcgc agggcgaggc
    13861 caagcaggtc acgagcacct acggctacaa tggctactac ctgtgtccgc cgcacgccgg
    13921 ttatcacgaa acgaagcgcc agccctggaa ggatattgac cagttatatt ggcccgaccg
    13981 gctcttcacg ttcggggaca cgctgatcga catgggcggg cgcccgcgga acaacaccca
    14041 cctggacccg cctttccgat actacgggcg gcgctggatg ccgaacggct ctccgacgac
    14101 ggcttttcgg cacaatgggc gcgccaacat cctgctggca gacggacacg ttgacgcctt
    14161 cggcctggaa ggcggaagcc tgcagaacgc agagtacagc atcggctcag tcggccccac
    14221 gaacgacccg cactacgtac ccgactggcg ggagtggagc aaatgaacgc aacaacccgc
    14281 cccgcgtcga gtggcaagtg ataaggtgat aaagtgatag ggtgataaag ggcgactcgc
    14341 ggaatcactt tatcactcta tcaccttatc actttatcac cctctagtcc gccgctccaa
    14401 atctaataga aactgcttgc gccacagccc gccgccatac ccgcggagct gcccgtcctt
    14461 ctgcaccacg cgatggcagg gcacgacgat cgccagccga ttgtcgccat tggcgcgtcc
    14521 cacggcccgc tgcgcgccgg gacaccccac cgcccgcgcc aacccctcat acgacagcgt
    14581 ctcgccaaac ggaatctgca gcaaccgccg ccaaacgcgc tcctggaacg gcgtgccgcg
    14641 aatcaccagc ggcaccgtga actcccgcaa ttcgcccgca aagtaccggg ccagctcatc
    14701 cgccagccgc tcaatgtgct cgttgcggcc gggcaccacc ggcgttccga agcgccgggc
    14761 gagcaccttc cactgcgtgg gcaacgcccg ccggtcctca aactcgagca ggcaaatgcc
    14821 ctccgagttg gcggccacct ccaggtaacc aaccgggctc tccagccggc tcgaaacaag
    14881 cgtatccaac atgcgtcgcc tcccgggcgg gccgccgaag gtgcggcgga acgcgtcgcg
    14941 gaacccgctc tcggattcaa acccgtactt cagcgccgtc tggctcagtt gcatcccgcg
    15001 ccgcaggtcg gccaatgcct gtcccaagcg ccagtgccgg caataggcct ggaaactcat
    15061 tccatagttg ctgcggaaat accgccgcgc ccgggccggc tcaatcccgc gggcgcgcag
    15121 ctcgtcgtcg ctaagcctct cgcccggcgc acgggccagg gcgtacagca aatcggaaac
    15181 ccactgcggc ggccggccat tggcatccag cggccggcaa cgcttgcacg gccgatagcc
    15241 ggccagcaac gcgtcgcgca ccgaggtcag gaaatccgtg ttccgcggca gcggcttgcg
    15301 ggccggacac gacggacggc agaagatgcc cgtactccgc acgcacacgt agaacagccc
    15361 gtcataagcc ttatcgcgcc gcgcaaacgc ggcctccatc tcgactctgc tcaacgtgcg
    15421 cttcatgcca ttctgctcaa cgtacgtttc atgccatttg ctcagcgtac gtttcacgcg
    15481 attctgccca gcggcgtttt catgttgcgc agcccaacgt gcgctttctg ccgctctgcc
    15541 cagcgtgcct ttcacatcat gagcatatca cgccgcagcc cgcccccacc accgaaaact
    15601 gagcgcggaa gtcaaatcag caggtcaaca ggtgacgaag cacgatctgc gaaatcactt
    15661 tatcacccta tcactctatc acattatcac cccgcgaacg ccggcgctga cagctcacgg
    15721 ccgaccgctt attctgcgcc catgaaactc accgattccg aaaagcgcat gctggccggc
    15781 gaagtgccgc tcgcgggttc cgcagccgcc gtcgcgaagg ccatggacta cctggttcag
    15841 ttcggcgacg ctttcggcgc cgagcgcttc gtcgacgtga tctacgccca ctatcccgcg
    15901 gaaatgtcga tctaccgcgg caacgtcgaa gacgccctcg aatacgccga aaccggggcg
    15961 cgggtctgca tcccgaccac cagctcaacg ctggcctgcg acctcgagca atggcgcgac
    16021 tatggctgcc cgcccgcgat ttacgagctt cagcagcgag tcgtcccagc gcaccgcaag
    16081 ctcggcatcc tgggcacgta cacctgcacg ccgcaactgc tcgggttcgt gccgcctaag
    16141 ggctcgtaca tcatcacggt cgagtcgtcg gcgattatct actacaactc cgtcctcggg
    16201 gctcgcacca atcgcggcgg cctcttcacg cgttacgccg ccgtgaccgg caagtacccg
    16261 ctgaccggct atctgctcga cgaaaaccgg cgcggcacgc acctcttcaa gctgcacctg
    16321 gagcgcgatg acctgcgcga gaattgcgat ttttcggcct tgggctttca cgtcggcgga
    16381 attgtcggca gcgaagtgcc ggtctttgac gccctgcccg ccggaacgac gcaggacaac
    16441 ctcatcgccc tcggcgccgc actggccacc agcggctcgg tcacgctgtt tcacgcgccg
    16501 ccccacacga ccgaggcccg caccgtcgct gaagcgctcc acggcgggcg gccgaaagaa
    16561 acgcatacgg tcacgcgtgc cgacctgcgg gccgtcttcg ataagctcac gaccattgcc
    16621 ccaagcggcc cggtcgattt cgtcacgctc ggctgtccgc actactcact cgaacagctc
    16681 cgttgggtgg cggattggtt tgtctcgcgc aatgcccgcg tgcacagcaa cgtgcgcctc
    16741 tgggtctgca cgaaccgcat gacgcgccgc gcggccgaat ggcagggcat tacgcagtcc
    16801 atcgaggctg cgggtgggcg cgtcatctgc gacacctgcc cggtcgaaag ccacatgcgc
    16861 atttcgacct gcaaggagca cggtttgccg acgccgcaga ttcaggccat ggtgaccgat
    16921 tcgtgcaaga tggcccgcta cgttggcgac ctgatcggct gcaagaccgc gctgcgcagt
    16981 cgcgaccagt gtttggccgc ggccgcagcc gggcggctgg tgtgagtgcg ccagccctgt
    17041 ccttggtgct acccccggac gctaagaaac taactgcgtc tgcgtccccg tttcatatgc
    17101 cgtgggaagg tgggaaggtg ggaaagtggg aaggtgggca cgtggttctg ccacggcgat
    17161 tcgctcgttg ccttaatgtg ggcccggtct tcgtcttccc ctccctggct ctcctctccc
    17221 ctggcgccaa gattcttccc ccggcaggcg ctcggcattc cgcgcacccg ctcggcgcct
    17281 aggcacccgt tcggcactac agctcttcgg ctcctccgta caccttcagg taccccacgc
    17341 ttgtacggcg ccgcggcggc cgcggggcgt gtggtcgccc gcggctccta taatcgcgcc
    17401 gttatggacg actgggccga acggccgaca atgtccttaa ggcgcacgca tggattgctt
    17461 cagataccaa aacggctcct tgtactgcga ggacgtggcc gccgccgaac tggcggcgcg
    17521 cttcggcacc ccgctgtacg tatattcgca gaacacgttt accaaccact accagcgctt
    17581 gcgccaggcg ttcgccgcgg tcgatccgct gatctgcttt tccatcaaag cctgccagaa
    17641 cgtacacatc tgccgcgtgt tgcgcgagct tggcagcggc ttcgacgtgg tcagcggcgg
    17701 ggagctattt cgggccctgc gggcgggagc ggatccgcaa cgcatcgtat tcgccggcgt
    17761 cggaaagacc gagcgcgagc tgaacgaggc gctggacgcg gggatcgggc tgttcaacat
    17821 cgaatccgag tcagaactgg ctgagctgac gcgcatttgc cgccggcgcg gcgtgcgccc
    17881 gcgggccgcc ctgcgcgtga accccgacgt tgacccgcat acgcacgagt acacaaccac
    17941 cggcaagaaa gaaacgaaat tcggcgtcga tctggaccgc gcggtgcgcg tcttccaccg
    18001 cttcgccccg cgcagcccgg tgcgcctgtg cggcattcac ctgcacatcg gctcgccggt
    18061 cttcgacgtc gccgcttacc ggcgcagcct ggagcgcggc atcgagctga tcgagcggct
    18121 gcggagcgat gggctggaag tggacacgat cgacatcggc ggcgggtatg cggccgacta
    18181 ccaggccgag gaggcgccgc ggcccgagga atacgctgac gccatcgtgc cgctgctgaa
    18241 gggccgcgac ctgcgcgtca ttctcgagcc cggccggtcg attgcggcca acgcgggcgt
    18301 tctgctgacg cgtgttctgc acgtgaagcg ttccggcggg cggcggttcc tgatcgtgga
    18361 tgcgtcaatg aatgagctca tccggccggc gttgtacggg gcatatcact tcatctggcc
    18421 ggtagcggcg ggcgatcgag ttcccgtctc gcgtcaggcg gagcagccgt tcgagggttt
    18481 ggacctgtcc gacgtggtgg gcccggtttg cgaaagcggc gactttttag cgcggcagcg
    18541 catgctgccg ccggtcaaac gtggcgacct gctggccgtc tacagtaccg gcgcctacgc
    18601 gatggtcatg gcgagtcaat acaattcgcg gccgcgcgcc gcggaagttt tggtcaacgg
    18661 cgggctggcg cggctgatac ggcggcgcga gacgtacgaa gacctggttg cggctgaaga
    18721 gctggaatcg gcataaacag tcgaacctgc aggtcggtga aagcactctg gtctgattcc
    18781 ggctcagcac gagccggctc tgctacgcac tatactcagg ccgggcggcg cggcagtggc
    18841 ttgcgcagca tgagagtggt tggcatggtc gggtactgca tgcgcagccg gtacagtttg
    18901 agctcgcgcc cgttccagcc ggtctgctcg agggcgtagc gcaacatctc cgggtagcgc
    18961 gggatatctg gagtgcgggc gacatcgggg ccattgccga gatagacgag gggatcgtgc
    19021 agcggcaggc ggtcggcggg ctcgtagcgc acgccgggac cgcccccgaa caggtcgcta
    19081 tacagctcag gcgtgggcgg ctcaccgccg ccaaagatag attcgtggac tagcagatcg
    19141 aagatcagca cctcggccgg cgtgcgcacc ggatacatga cacacaaatc cgggtacagc
    19201 tccgtgcgat aacgcggttc gatccggcgc accaactcgc ccgtcacgca agtcatacga
    19261 ccggtattgc ccacccggct ctcaacgaat tcgaactcca cgtcgccgcc cagcccgatt
    19321 acgggccgga actgcggcac cggctgcgag cagaagtcga tcagcagcgg cactccatca
    19381 gcggcgcgcg gatcgagggc cccacgcgat aattctgagt ggacgttatg gctgcgatcg
    19441 accgaggcgg gacgcgcgat gcgccaggcg accttggggc gcatgcaccg gaaatcgatg
    19501 aaaccgcgga cggtcgccag atcccacgtt tcgagatcac tcgagggatg cacaatgttg
    19561 gcgcggaaga tggttcgggc ccgcacgccc cacacatacg tgttgccctc gaacgccagc
    19621 cggcgatgtt ccaggtccgc ccgcatctga tcggtcccgg ccagcccggc cgcaagcatg
    19681 tcgaaggatt tgcgcgttcc gccgtgagtc tgcacgagct tgcggaagtc ctcgaacgcc
    19741 gcgcgggcca catcgagggc gggcttgggc acgccgcgcc gctgggcggc gcgcaggaaa
    19801 gcgcgcattc cagttggtcc gggaacaaac tgcgccgccg cgaacgggtc ggccacgtcc
    19861 acgcagcgcc cgaccttcca ggccaatttg gtggccaggc ccagatcggc cgccaggtca
    19921 ttcggccgcc ggtaagtcag tcctgggacg cgggcaatga cgtccgccat ggagtcgcgc
    19981 aggcggcaaa ccacgctctc aacgtcggcg gcaaaatcca ttcgttccgg ctgggcggtg
    20041 gccgtgctca tgattcatcg aatatacgga aaaacagcta tttggtgcaa ggttagagcc
    20101 gtcgatacgg ctcgacggca tatgaaactt caattgctat tgcaaaccgg tccccggccg
    20161 gttagagtcc gcaattggcg aaggacggtc gtactcggac cgggccggct gccaatcagc
    20221 gggcactgct tcgcccgata atccagggca ggcagtttcc tgttgaggtg gttagcgaaa
    20281 acatcgcgcc gcgcgcgtgc tgatactcgc cgccaaatgg tccagggagg ctgggttgct
    20341 gcaaggacgg ccgggaacgc cgaagggcgg tcccggccgt cactatgttt aaacggtgct
    20401 agtccacata ccggtacgcc acccacaccg aaatcagcgg gtcgtttccg ctgacgtcga
    20461 ttaccacgga ctgcccgcgc tcaccctgtg gctggtgatc gggccagcgc agatcgttaa
    20521 gcgtggtgca gcacatgttg ggcagaatat cgtttgagtt gctttgtacg cgcaacacct
    20581 ttgtccccat gacggtgccg cgcatcgact gcgtcagccg gtcgattttg atgattttgc
    20641 cctcgactgc gggaatctcc aacggcgacc cgagcgcctg gaacattgtg gccattcgaa
    20701 cacctctcac ttgccaaagg cggcaacttc aacgagttgg gggcgcacgc gcgcccctca
    20761 gttcggcaaa tgttagcgat gcgcaacggc cgcccgccga ccggccagtc tgaatgtgcg
    20821 ccgctgcgcc tgtttgagcg tcattgtcag atttgcagga ccgctcgcag ggcgcgccgg
    20881 cccggctttc gcccgccagc caccgctcgc cgcgcccgcc cgcgccgaac aaatgatgag
    20941 cgcaatcctt gcgcagaacg ccggtctgag aactattgct cggcccaaaa ctacgtcgcg
    21001 cgtccctgat gagacgataa cagcgccggc agcgtttgta tcaccgctgc ccttctcggc
    21061 tgccatggag ggcgccgaaa ccccagcggc cgaggcggct tggcgcgggc aggaggccct
    21121 cgtccggttt gccggttcgg gcatggaggc ctaagccggt gaatcgcccc tcgccgccgg
    21181 tacgcgagcc gccacgtccc tgggctgtcg ccgcctttct cggccggcgc cacccgtctc
    21241 gcggccactc tttaagctgg ctgtgcgact tggacaaagc ctgtataatt ctcgtcacga
    21301 tggcgctgtt cgcccgaccc agccaagcga tccgttcggc tggcgtgccg gccgaaagcc
    21361 ggggacggcc gctcggtgaa gccgcaccat cctgttctta gccgattgac agaggagcga
    21421 atggaccagg tcgaaatcgt cacgcggcag gcggatctgg aggcatattg taggaattgg
    21481 cggcggctgg gccgcttggc tttcgatacc gagttcatcc gcgacgagac ttacgagtcc
    21541 gccctctgcc tgattcaagt cgccgccgat ggtcaggtcg ttctgattga tccgaccgcg
    21601 cccatcgacc tggcctcctt ctgggatctg gtcaccgacc ccacggtgac caccatcgtg
    21661 catgccggca aagaggattt tgaagtctgc ctgcgctcca ccgcccggac gccgtgtaac
    21721 gtcttcgacg tgcagatcgc cgcgggcttc ctgggctacg gctacccgct gagcctgacg
    21781 cgcctcatgg ccgacgtctt aggtcagcgc atcagcaaag gccagacgct gaccgactgg
    21841 ct
//
LOCUS       DAOSYS010000030        21742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1969_length_21742_cov_5.984184, whole genome shotgun sequence.
ACCESSION   DAOSYS010000030 DAOSYS010000000
VERSION     DAOSYS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21742
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_21742_cov_5.984184"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLU99_03215"
     CDS             <1..201
                     /locus_tag="PLU99_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOO16098.1"
                     /translation="RVFTSGVLKWQDQPVEIDIPVAGAARLVLRADPTADGQRADHVN
                     WALARFEMGASATTQPSYETGP"
     gene            complement(321..1265)
                     /locus_tag="PLU99_03220"
     CDS             complement(321..1265)
                     /locus_tag="PLU99_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015112032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV damage endonuclease UvsE"
                     /protein_id="HOO16099.1"
                     /translation="MVRRSPTTAEGPHRPSQPARTGPRLGLVCLSAGPQVRYRTVTRT
                     RYLALAPSQRRRTLHELYSENIRRLYGALEFCSARDIHLYRVTSDLFPMDFALSERVL
                     DELVPELSGFGRRAQALNVRVVAHPDQFVVLNSLSRRVARQSAAIFTRHARVFDRLGL
                     PRSPWAALILHGGKSGRAEALLAAIAALPEAARSRLVLENDESAYGAAEILAICKAAG
                     VPMVFDAHHHLVQAGMRDYNDPSYAQFVAAARETWPRPEWQIVHLSNGATGVRDPRHS
                     EYIRRVPPAYHDVPWIEVEARGKELAIARLRRMWPAAR"
     gene            1386..2816
                     /locus_tag="PLU99_03225"
     CDS             1386..2816
                     /locus_tag="PLU99_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOO16100.1"
                     /translation="MPTITEKIARWAAEVRYEHLNPAAIDAAKRFLYDTLGCALGGAQ
                     VHDCKIFLEHYRGLQQPGPCTVIGSGDKMNPVAAAMLNALMVRAMDYNDIYWKQDPSH
                     PSDLASAPLAIAEWKHLPGKDLLLGLVLGWDFTMRLCHIAVPGIRERGWHHATLMAYA
                     TPVVAGKMLGLSWQQIQHAIGIAACHRFTLGCAVAGKLTMMKNTVSPMATRDGVEAAL
                     LAQRGYTGPEGVFEGKEGMEHCLSKGYPGEGKSWDYTWITDKLGSTLEGGNDPANGIG
                     WMITQCGMKGYPIEALMHSPVSATLHIMKANNLKAEDVKSVLIESIARAADILSDPAK
                     YDPQSKESADHSLPYCIAAALAEGRVTPLEFKEDKLWDKRLRAQMPKVKVVANPEFEK
                     AFPAKQCTRVTITTNDGKTFTHAMDYPKGDPRDPMTVEEIGVKFNALASPIMSESRQQ
                     ALKAAIFDLENMKDVAELMKLTVADK"
     gene            complement(2958..4928)
                     /locus_tag="PLU99_03230"
     CDS             complement(2958..4928)
                     /locus_tag="PLU99_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO16101.1"
                     /translation="MRTKGDQGLVLLTVLVSLALASLTVWAVARQGHALQERDWADLR
                     NAARAAADEQSIALQADIARALDAVAARLRSEGLAGLECWLDEQQDWNFIVLCAEDQE
                     VLLPQTPPTGRPTSAAAPELPRYEPDLDEDEVRGVLMACQPLLAAEDVSLRVRACLTA
                     ADCYRHLGDTASAVRVLESAGQLLSARTAAEPESFEALVEVIGCLAEAEAREPARKYL
                     QALLQQIAGAHPARYTPEQLEFLKRRTIGIVDGSDVHDLRQQLEDAALRASIRRAAPD
                     VLAAARAACRARPDSGEQSFTLQTIPVQGAPPQLMASRQAGVDGCIVLAQSAMKALAR
                     YWPFNPAQHWRLARTPDPQDVILAQLDDSLGGAALVASQPTLRLMRGQARWRTALVLV
                     TAAATAGAWGVVIWMMLRAMAHQRELVRLQRRFVADVSHELKTPLAMIRLLGETLLDG
                     RVTDPQRVQAYHGTITREAERLSVLLDSILDFSRIESGRRQYVFEPCDFAAVARQAWV
                     LFEPQFAAAGFEHHLEIEPNLPVVPADAAAVQQVIVNLLQNAFRYGGEGKYVRLAVNR
                     DGYVIVVTVEDHGIGMTRSQLQRLGDTFFRAEDTRVRQTRGSGLGLAIVNHIVQAHGG
                     KMEVHSRPNRGSKFTVWFPFEPRAVTANSRPA"
     gene            complement(5000..6607)
                     /locus_tag="PLU99_03235"
     CDS             complement(5000..6607)
                     /locus_tag="PLU99_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16102.1"
                     /translation="MKCARIAAALILLVVTLGSLTVSGGYAWYYRSDRYRRQCVADLS
                     AALGLPCDIGRVLPRARCCREYNDVAVWLPDRRDKVLSCPRALLTRLNDAADERAYEM
                     RLLGGACEISTRTWLREDYRRVVESGLRLGFAPDGPRQIIIQGMDLRFIRDDFVALLE
                     DATGSVTFDSHERGRASIQCRSLNGYASPESITLTMRFSPLDGGVQIDELTLHAPNLP
                     LNVLGLGALTGLLIESGRFSGQLAYSEADGRRRVTLAGRCYDLSLPECTAGFASPGWR
                     GAGREIQLQELTFIDGRPVRLRFSGALTGMVLGDLLAPCGMQAADGQTDLRIAAAELS
                     PRGIDRLVASGKCGNISLRALSERCAAGRVSGRATLVITDLTIRDNHLASLDAEFHVE
                     DGPEPKWIEGRLLTNLLGRILDIELPPVLPERIEYTQLGARLEVRDEVLYLFGTHGTR
                     GKTILTGKVMEHEIPLIWEPEQGRDLAGWFDALRTQAAAYVHRWQSLPPRQAWQAFST
                     LRGAPPPPAPSAPTTSPAPGAVPAAAP"
     gene            complement(6728..7114)
                     /gene="acpS"
                     /locus_tag="PLU99_03240"
     CDS             complement(6728..7114)
                     /gene="acpS"
                     /locus_tag="PLU99_03240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015147439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOO16103.1"
                     /translation="MVAIVAHGIDLVQIERIARVWREHGERFLNRIFTAGERNYCLGG
                     RNAAERLAGRFAAKEAVMKVLGTGWRGEIRWTDIETLPDALGKPLVTLHGACAQRAAS
                     LDIGRILLSISHTREHAIASAIALSV"
     gene            complement(7167..9128)
                     /locus_tag="PLU99_03245"
     CDS             complement(7167..9128)
                     /locus_tag="PLU99_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOO16104.1"
                     /translation="MNEQTTTPAARATSQTQPNPSVRAGPARLVIGIVFLAVAIVAAA
                     MMALEHLAGLRLPGCGPGSPCAEASQSAWGSVPYVNWPVSYLGLAYFAGLLVAWVSIC
                     RHGAPLLLLWLVRLGLLVSVGFVLIMLLGGYPCPYCLATHLSNLGFWILIETLPRAAA
                     PRWQPAAALAGMFVLATAALAVLETSVQRELQTVAEADAQATAAAIVAGSSQPIAESS
                     VKPPITEVGQPLGPAPAQPTGSAASAPAGSAAAASTAEPLRRGFTGRYRLGPEAVPLR
                     LVIFSDYTCKGCNLLEIELKQLLATSDIVSLSVKQCPMNSECNHLFSSEREHENACRA
                     ARAAEAAGLLRGNDGFWQMHHWLFDRSGRFTDDELRTALAEFDYDAAEFERVMNSDEV
                     LQLIQADVQEAFDLGMTTTPMVFINGREFKGWSVPLNLTKLVETLKTKNLPAKTAEAD
                     QPAPAPQRYVNEWKESRVRDFPPAARAWTNGSPMATLPIIAWGDYLQPNTALADAIIR
                     EFLTSHPEAQYTFRHYPLSPECNPAVDEERYNQSCLAARAAETAGRLNGQDGFWKMHV
                     WLMENQANFSEQTLQDALPELGFEPLAFLDAMNDPAVTEAILDDARAAQEQQLRNAPL
                     MFVKNKVIPRWLHGSRPLLREILEAAARE"
     gene            9310..11376
                     /gene="fusA"
                     /locus_tag="PLU99_03250"
     CDS             9310..11376
                     /gene="fusA"
                     /locus_tag="PLU99_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO16105.1"
                     /translation="MEDLSKIRNIGIVAHIDAGKTTTTERVLYYSGTIHKMGEVDYGT
                     TVTDFDVEEQQRGITIYSAAVSFQWRDCRVNLIDTPGHVDFTAEVERSLRVLDGAVII
                     FDAKEGVEAQSETVWRQADKYKVPRLCFMNKMDKTGADFEHAVATVRDRLAGNPIAIQ
                     LPVGRENTFRGHIDLIRMKAVFYHGGDGRNFELVDIPADLESEARQARRELEEKLAEL
                     DDHLMEKYVEGRPFEEGEMRTALRAGTISLRCQPVLCGSSLRYMGVQALLDAVTDYLP
                     SPLDVPPISGFDPDKPSKIVQRKCDPGEPLAALVFKVIAEPHADLHFLRIYSGVLKAS
                     SRVVNVGRKKKENVPQLFHLFAKRREKIERAMCGDIVAVVGLKETLTGDTLCDSRAPI
                     LLERIEFPETVISMSIEPKSSADRDKLVNALKMLSRSDPTFDFTVEPETGQTLIHGMG
                     ELHLEVMCHRLERDMNVDVRVGKPRVAYRETITQAAEAEGRIARQVGGRAQFAVVRLR
                     VEPFQPAPGQEHFRFENAAPADELRPQFAAAVEQGVRGAAHSGALGGYPLINVKVTLL
                     GGQEHAEESTEVAFESAGALAVQRALEQAGPVLLEPIMRVEVVTPEEYFGAINGDLMS
                     RRATITGTAMRARDRVISAEVPLATMFGYATQIRSLSQGRASYSMEPYRYEPLPQELV
                     KKVLGI"
     gene            complement(11546..15178)
                     /locus_tag="PLU99_03255"
     CDS             complement(11546..15178)
                     /locus_tag="PLU99_03255"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16106.1"
                     /translation="MRWRLFVVTMSSCVVPLAFSGVDERAARAALQRANPDARVSFEG
                     RRVNRVFGAPLSFGTSPQESAANFLQQHAPALGVTSAELRAGSNFTGLPTVPLMPDAD
                     RGGNKFTLVNFAQEKDGIPVFRGEVRLLTRNEPGFPVVLVTSSARNLGDFVVDRGVAA
                     KPFDRLEQIAPEMTNYSDIQVVIWAGIDDAQVEPVLAITFTADNYDNPNAKPERWLYV
                     ADAVTGNVLYKEDLIRFAPITGHVQGMATEGSKADICSPELITVMPWARVSVSGGGTG
                     YADGDGNFSIPHSGSSPVTVQSFMTGTYFNVDNWAGAEETLSATVTPGVPYTFTHNEE
                     NISDLVRSQVNCYISANRVRDWILAQNPEFPGISTETNFPIYVNRTDGYCPCNAWSDG
                     ISINFCQAGDGCPNTGWQSVLDHEYGHHVIDQGGSGQGAYGEGMSDCIAVLTVDDPNL
                     GYGFFGDCDAGLRTADNDCQYLASGCSTCGSEVHDCGNLLSGCIWSIRNELIETEPAE
                     YLSILSSLTVNSILLHLGTSINDDIVIDFLTLDDDDGYLGNGSPHYNEICAGFTAHGL
                     NCPELVTGIRVAPESVFRSEGHVGGPFISSGVFVVRNIGTYDIDYSVTCPQDWVSISN
                     GSGTLEGGASTLVTVSINSQAASLPMGVHNATLSFENTTDSVGNTTRGVELAVGYGTV
                     YSWNMNTNPGWTTQGQWAWGVPTGSGGGGGGPDPTSGHTGRNVYGYNLNGDYTNNMPE
                     YHLTTPPIDCQGLTDVHLRFSRWLGVEKSIYDHAYVRVSTNGTSWTNVWQNGAEDVAD
                     TSWSLQDIDISSLADNQPNVRIRWTMGTTDGGLTFCGWNIDDVAIFAAGDFTLPPLVL
                     SLPLDPPSIVPALTPTPLTLHISSAGETYVPGSARLYYRFAPGAFSEVTLTSLGGDLY
                     RAVLPAAPCGAQPEFYFSATGSDGATVVLPENAPTELYRVGVGTLTTIVFDDFEAPSG
                     WTVGDTGDDASAGIWDRADPNPTAAQPGSDHTPAPGTMCWVTDSRGGALGSYDVDGGK
                     TTLKSPNYDLSGSTYAVIGYWRWYSNDQGATPHTDVFVVDISDDGGSTWVNAETVGPT
                     GPETSPGWFYHEFNVQDFVALTDQVRLRFIASDEGDGSLVEAALDDFSIATLNCDDTW
                     QPGDLNCDGLINAFDIDPFVLALTDPGAYGSAYPGCNHMLADVNGDGTVNAFDIDPFV
                     LVLTGG"
     gene            complement(15435..16475)
                     /gene="queA"
                     /locus_tag="PLU99_03260"
     CDS             complement(15435..16475)
                     /gene="queA"
                     /locus_tag="PLU99_03260"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOO16107.1"
                     /translation="MRIQDLQYDLPPELIAQRPAEPRDASRLLVLERRTGRVQHRRFS
                     DVCAFLNPGDVLVVNDTRVIPARFFCRRQTGGLIEGLFLHGDDAGWSVLLKPSARLAT
                     GERLKCAGRAVELVLIERRPRGQWLVRPEPAVPWLELLESIGQTPLPPYIRPDSAGRT
                     GTPDASDRERYQTVYAERPGAVAAPTAGLHFTPALLRTLEQRGIRVARVTLHVGLGTF
                     APIDVEDLAEHKMHSEWFDIQGQALATIRAVRSAGGRVVAVGTTAVRVLESLSTLEGT
                     GQEGWTDIFIYPPYSFRHVDVLITNFHLPGSTLLALVMAFANPEQIRAAYNEAITQRY
                     RFFSYGDAMLIL"
     gene            16643..17764
                     /locus_tag="PLU99_03265"
     CDS             16643..17764
                     /locus_tag="PLU99_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16108.1"
                     /translation="MADWGEAEVRGRCGCGRRYRILHAKPDQVVTCPNCGRGIIVTRA
                     DLKAAGAGEMLIPLQPEQVEVREALLVDEVELRPAPAGSGPGLTGRQVHTHEVALLAE
                     ALEPSRQIVHGAGAYSPGAPRSQPAAGAPVRPAAFLLDMLASFWFAGRPRNALVLAIT
                     ALVFTVPLLILGRPVIGYVVFLLTALLEFLLFLHALQFFWHVMTETAMGEDDLPVVPR
                     GWDWIEDALKPLGWLLLITLLCSVPAIAAGWYAPPGQYREPLIWAAVLLGTFVWPIAL
                     LSVALANGRVLLRPDLLVRSVWGIGPAYIAAWFLVLALVAAIVIFVGVGPAAMGWPAV
                     VATVYPIATGAMVFYLAYVLFRTLGLLFRHFGGRLPWRV"
     gene            complement(17793..18407)
                     /locus_tag="PLU99_03270"
     CDS             complement(17793..18407)
                     /locus_tag="PLU99_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16109.1"
                     /translation="MPIQFRCSGCGQPIEVDDEHAGKMAACPYCNRVVAVPLASSLEE
                     QPIAAARPTASSGQAEEGWSPPPPETARPLDAREQTGRKYGRYALICLALAGISMVAL
                     LVLSWPALKEIARVSASQPGQATGSQLEELEEIARGVQSRTEIVGLSCAAELFIVIAL
                     ALSITSVVQCRRDNVPGIVALVLSALLTACVCFSMLFNLATMSG"
     gene            18617..19807
                     /locus_tag="PLU99_03275"
     CDS             18617..19807
                     /locus_tag="PLU99_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16110.1"
                     /translation="MDQNAPVLIGVDGGASEVKAHAVACDDLSRPSAFVLRPESAARV
                     YARDPGFAPLPVAEQIAQHRSGRCDISPAERQQGELWLQAAADAICDVARQCGSRRAL
                     IGIGMPGLKTADARGICVINNGPRIPDYLDELERRLAADGLELAAPIAELGSDADYCG
                     LGEEYAADGLFRDVQNAYYIGCGTGIADALKLGGRLVPFDQAKSWILKSWQIPSALGP
                     TFEQLISVAALNRVYAALIGDSGPSAYPERAAAAGEPRACMWMATAGLVLAELIFERL
                     WTIKNGRAQAAHRGENYLQLNPEHEFRGTVLQRVVIGQRLGQIWADPAFGDILRCKLE
                     VALAARLVECGDADLMSACLVATPQFRIVPPHLLRPSRLRAAPALGAAVAALRASGEL
                     GKGT"
     gene            19921..20343
                     /locus_tag="PLU99_03280"
     CDS             19921..20343
                     /locus_tag="PLU99_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO16111.1"
                     /translation="MIRSALVIVGAAVALSLTACQKPQAQPEAHVEFTPAPTLDQSER
                     TPVQPLDAYVATPPPVYNPEPAYTPPPEPVIKPADKPVRPAHRERARTYVVKKGDTLS
                     EISQKFYNTPNRWRQILKANPHVKSPKKLQVGTKLVIP"
     gene            20455..>21742
                     /locus_tag="PLU99_03285"
     CDS             20455..>21742
                     /locus_tag="PLU99_03285"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO16112.1"
                     /translation="MYLLRPDSPVQYLKGVGPQRAAWFEQAGIKSITDLIEYFPFRYE
                     FDTGLVDIADLQPRTTATVRGTVLYTHGRGPYGSYTAEIDDGTQTCTLRWFQSKYVPR
                     GLCVGASVIASGDVQEFNDRLEIVQPRVQVFPPNTPVEVPRRGARMVGVYRAAGKLTS
                     RVVQRAVEAALSAPQLPVEEFLPADLLKKHNFPARGEAIRQMHSPRGDEQLRRARERL
                     AYEEFLLLELAMALRRQRTVVLQPGQKLHVTPEIDRRIRARFPFELTSSQNEVVSEIA
                     ADLSSGRPMTRLLQGDVGSGKTVVSLYACLVAVANRKQAAIMAPTEILAEQHFRNIEQ
                     YLAGSRVRYVLLRGGLSRAERTKILASIEAGELDLVVGTQALIQEDVSFKNLAVVVVD
                     EQHKFGVMQRARIRTKGPMPHYLVMTATPIPRTLAMT"
BASE COUNT         3918 a         6817 c         7167 g         3840 t
ORIGIN      
        1 cgcgtcttca caagcggcgt gttgaagtgg caggaccagc cagttgagat cgacattccc
       61 gtggccgggg ccgcgcgact ggtgctgcgg gccgacccga ccgcggatgg ccagcgcgcc
      121 gaccacgtga attgggctct ggcgcggttt gaaatgggag cttctgcgac aacccagccg
      181 agctatgaaa ccggcccgtg aacatggacc gggtgtgcgc ctggccgcga ccacgtgata
      241 aggtgataaa gtgatagggt gttaagggcg atccgcgatc cctttatgac cctatcactc
      301 tatcactcta tcaccctggt ttaccgcgcc gcgggccaca tgcgccgcag ccgcgcgatc
      361 gccagttcct tgccgcgcgc ctcaacctcg atccacggca catcgtgata agccggcggc
      421 acgcggcgga tgtactcact atggcgcgga tcgcgaacgc cggttgcgcc gttcgacagg
      481 tgtacgatct gccactcggg gcggggccag gtttcgcggg ccgccgccac gaactgggcg
      541 tagctcgggt cgttgtagtc gcgcatcccg gcctgcacga gatggtggtg cgcgtcgaac
      601 accatcggca cgccggccgc cttgcagatt gccaggattt ccgcggcccc gtaggccgac
      661 tcgtcattct cgagcaccag ccgcgaacgc gcggcttcgg ggagggccgc gatagcggcg
      721 agtagtgcct ccgctcgccc gctttttccg ccgtgaagga tgagcgccgc ccagggactg
      781 cgcggcaggc cgagccggtc gaacactcgc gcgtggcggg tgaaaattgc ggcgctctgg
      841 cgtgcgacgc ggcgcgagag cgagttaagc acgacgaact ggtcgggatg tgcgacgacg
      901 cgaacattca gcgcctgtgc ccgtcggcca aagccgctca attccggcac gagctcgtcc
      961 aacacgcgtt cgctcagggc gaagtccata gggaacaggt cagacgttac gcggtaaaga
     1021 tggatgtccc gcgccgagca aaattcgagc gcgccgtaga gccggcggat gttctctgaa
     1081 tacaactcgt gcagggtgcg ccgccgctga ctcggcgcca gggccaggta gcgcgtgcgt
     1141 gtaaccgtac ggtagcgcac ctgcggcccg gcggagaggc aaaccaagcc gaggcgcggg
     1201 ccggtgcgcg cgggctgcga gggccgatgc ggcccttccg cggttgtcgg gctgcgtctt
     1261 accatagtgc ttgatcgtag ggtcgcacgc ctccgcccgc tgcttacggc acacggcgtg
     1321 cttgtctggg aaggaaccgg tcgggtccgc ggtcccgacc tacacgaagg aacgggagct
     1381 aacctatgcc gacgattact gagaaaatcg cccgttgggc cgctgaagtt cgttacgagc
     1441 acctgaaccc cgccgccatt gatgccgcca agcggttttt gtacgacact ctgggctgcg
     1501 cgctgggcgg agcgcaggta catgactgca agatcttcct cgagcactac cgcggcctgc
     1561 agcagcccgg gccgtgcacc gtgatcggtt ccggcgacaa gatgaacccg gtcgcggccg
     1621 ccatgctcaa cgccctgatg gtccgggcca tggactacaa cgacatttat tggaagcagg
     1681 acccgtcgca cccgtccgac cttgcctctg cgccgctggc catcgccgaa tggaagcacc
     1741 tccccggcaa agacctgctc ctcgggctgg tgctggggtg ggatttcacg atgcggctgt
     1801 gtcatattgc ggttcccggc atccgcgaac gcggctggca ccacgccacg ctgatggcct
     1861 acgcgacccc ggtggtcgcc ggcaagatgc tgggcctgag ctggcagcag atacagcacg
     1921 cgatcggcat cgccgcgtgc caccgcttca cgctgggttg cgcggtagcc ggcaaactga
     1981 ccatgatgaa gaacacggtc agccccatgg cgacgcgcga cggcgtcgag gcggctctgc
     2041 tggcccagcg cggctacacc ggtccggaag gcgtgttcga gggcaaggaa ggcatggagc
     2101 actgcctgag caagggctac ccgggcgagg gcaagagctg ggactacacc tggatcaccg
     2161 acaagctggg ctcgacgctc gaaggcggca acgacccggc taacggcatc ggctggatga
     2221 tcacgcagtg cggcatgaaa ggctatccga tcgaagccct gatgcattcg cccgtttctg
     2281 ccacgctgca catcatgaaa gcgaacaacc tgaaggccga ggacgttaag agcgtgctga
     2341 tcgagagcat cgcgcgcgcc gcggacatcc tctccgatcc ggccaagtac gacccgcaga
     2401 gcaaggagtc ggccgaccac agcctgccgt actgcatcgc cgcggcgctg gccgaagggc
     2461 gcgtcacgcc gctggagttc aaggaagaca agctgtggga caagcggctg cgggcgcaga
     2521 tgcccaaggt gaaggtggtc gcgaaccctg agttcgagaa ggccttcccg gccaagcagt
     2581 gcacgcgggt cacaatcacg acgaacgacg gtaagacgtt cacgcacgcg atggactacc
     2641 ccaagggcga cccgcgcgac ccgatgacgg tcgaggagat cggcgtgaag ttcaacgccc
     2701 tggcgtcgcc gatcatgagc gaaagccgcc agcaagccct taaggcggcg atcttcgatc
     2761 tggagaacat gaaggacgtg gcggagctga tgaagctgac ggtggcggac aagtagcggc
     2821 cgagggctca accgcgcgca cattcgtgcg cagctttgga aatccgcgtt ggtcggcggt
     2881 gatgtgcgcc gcccgcagcg attagcgtcg cggcgggcgg cgcttaagtt tttctgctcg
     2941 tgcccccggc cctgctttca cgccggccga ctattcgcgg tgacggcgcg cggctcgaag
     3001 gggaaccaga ccgtgaactt gcttccacgg ttggggcgcg aatggacctc catcttgcca
     3061 ccgtgcgctt gcacaatgtg attgacgatc gccaggccca gtcccgagcc gcgcgtctgc
     3121 cggacgcgcg tgtcctcggc ccggaaaaag gtatcgccca gccgctgtag ctggctgcgc
     3181 gtcatgccga tgccgtggtc ctcgaccgtg accacgatca catacccgtc gcgattgacc
     3241 gccagacgca cgtacttgcc ctcgccgcca tagcgaaagg cgttttgcaa caggtttacg
     3301 atgacctgtt gcaccgccgc ggcgtcggcc ggaaccaccg gcaggttcgg ctcgatttcc
     3361 aggtgatgct cgaaccccgc cgcggcaaac tgcggctcga acaataccca ggcctggcgc
     3421 gcaacggccg caaaatcgca cggttcaaac acgtactggc gacgcccgga ttcgatacgg
     3481 ctgaagtcga ggatactgtc caacaatacc gagaggcgct cggcctcgcg cgtgatggtt
     3541 ccgtggtaag cctgcacacg ctgcgggtca gtgacgcggc cgtcgagcag cgtctcgccc
     3601 agcaggcgga tcatggccaa gggcgtcttg agctcgtggc tgacatcggc cacaaaacgc
     3661 cgttgcaggc gaacgagttc gcgttggtgg gccatggcgc gcagcatcat ccaaatcacg
     3721 acgccccagg cgccggcggt ggcggcggcc gtcacgagca ccagggcggt gcgccacctt
     3781 gcctgaccac gcataaggcg gagggtcggt tggctggcga ccagcgcggc tccgccaagg
     3841 ctgtcgtcca attgcgccag gatcacgtcc tgcgggtcgg gcgtgcgcgc caggcgccaa
     3901 tgctgagccg gattaaacgg ccaatagcgc gccagggctt tcattgcgct ttgtgccaag
     3961 acgatgcagc cgtccacgcc ggcttggcgg cttgccataa gctgcggcgg ggcgccctgg
     4021 accgggatgg tttgcagcgt gaaagactgt tcgccggagt cgggcctggc gcggcaggcc
     4081 gcgcgggcgg cggcgagcac gtcgggggcc gcgcggcgga tcgaagcccg caaagccgcg
     4141 tcttccaact gttggcgtag atcatggacg tccgatccat ccacgatgcc aatcgtgcgg
     4201 cgtttgagga actccaactg ttcgggcgtg taacgcgccg ggtgggcgcc ggctatctgc
     4261 tgcagcaggg cctgcaagta ttttctcgcc ggctcgcgcg cctcagcctc ggccaggcag
     4321 ccgatcacct ccaccagagc ctcgaacgac tctggctcgg cggccgtccg ggcgctgagc
     4381 aattgccccg cgctttccag gacccgcacg gcggaagctg tgtcacccag gtgccggtag
     4441 cagtcggccg cggtaagaca cgctcgcacg cgcaggctga cgtcttccgc ggccagaagc
     4501 ggctggcaag ccatcagcac gccgcggact tcgtcctcat cgagatcagg ttcgtaccgg
     4561 ggcagctccg gcgcggcggc actagttggc ctacctgtcg ggggcgtttg cggcagtagg
     4621 acctcctgat cctccgcgca gagcacaatg aaattccaat cctgctgctc gtccagccag
     4681 cattccaggc cggccaaacc ctcgctgcgc agacgcgcgg cgacggcgtc gagggcgcgg
     4741 gcaatatcgg cctggagggc gatgctctgc tcatcggcgg cggcccgagc cgcgttgcgc
     4801 agatcggccc agtcgcgttc ctgcagcgca tgaccttggc gcgccaccgc ccacacggtc
     4861 aggctggcga gcgccaggct gacgagcacg gtcagcagca ccaggccttg atcacctttt
     4921 gttctcaccg gctctccgag ccaatgcttg aacccttgca gacggccgct gaggcatagg
     4981 cctgtaccac actggcccgc tacggcgccg cggccggcac agcgcccggc gcaggcgagg
     5041 tagtcggcgc gctgggggcc ggcggtggcg gcgctccgcg cagcgttgag aacgcctgcc
     5101 aagcctggcg cggcggcaat gactgccagc ggtggacgta ggcggcggcc tgcgtacgta
     5161 gagcgtcgaa ccagcccgcc aggtcgcggc cctgctccgg ctcccaaatt agcggaattt
     5221 cgtgttccat caccttgccg gtgaggatcg tcttgccgcg cgttccgtgc gtgccgaaca
     5281 ggtagagcac ttcgtcacgc acctcaagcc gcgcgcccaa ctgcgtatat tcgattcgtt
     5341 ccggtaggac cggcggcagt tcgatgtcca gtatgcggcc caaaaggttg gttaacaggc
     5401 gcccttctat ccacttgggc tcgggaccgt cctctacgtg aaactcggcg tccagcgagg
     5461 caagatgatt atcgcggatc gtcaggtcgg tgatgaccag tgtggctctg ccgctcaccc
     5521 ggccggcggc gcagcgctcg ctcagggccc gcagcgagat gttgccgcac ttgcccgagg
     5581 cgaccaggcg atcaatgccc cgcggcgaca actcggccgc tgcgatgcgc aagtcagtct
     5641 ggccgtcggc ggcctgcatt ccgcacggcg ccaacaaatc gccgagcacc atgccggtaa
     5701 gcgcacccga aaaccgcagc cggacgggcc ggccgtcgat gaaggtcaac tcctgcaact
     5761 gaatctcgcg gcccgcgccg cgccagcctg gcgaagcaaa accggccgtg cactcgggca
     5821 gactcaggtc atagcagcgt ccggcaagcg taacgcggcg ccggccgtcg gcctcggaat
     5881 aggcgagttg tccgctgaag cgcccggatt cgatgagcag gccggtcagg gcgccaagtc
     5941 cgaggacgtt caagggcagg ttcggcgcgt gcaacgttag ctcgtcgatc tgtactccgc
     6001 cgtccagcgg ggaaaagcgc atcgtcagcg tgatcgactc cggcgaggca tagccgttta
     6061 gcgagcggca ctgaatcgag gcacggcctc gttcgtgcga gtcgaacgta acgctgccgg
     6121 tcgcgtcttc aagcagcgca acgaagtcgt cgcggatgaa gcgcaaatcc atgccctgga
     6181 tgatgatctg gcgcgggccg tcgggtgcga agcccaggcg caggccggat tcgaccaccc
     6241 ggcggtagtc ctcgcgcagc caagtgcgtg tcgatatttc gcaggccccg cccagcagac
     6301 gcatttcata ggcccgctca tcggcggcgt cgttgaggcg ggtgagcagg gcgcgcgggc
     6361 aggaaagcac tttgtcgcgg cgatccggca gccagaccgc gacgtcgttg tactcgcggc
     6421 agcagcgggc gcgcggcagg actcgcccga tgtcgcaggg caagccgaga gcggcgctca
     6481 ggtccgcgac acactggcgg cggtaacgat cgctgcggta gtaccaggca tatccgcccg
     6541 atacggtcag ggaaccgagc gttacgacga gcaatataag tgccgccgcg atacgggcac
     6601 acttcatgga gggggattgt acggattgca cggcgggcgt ggtagggtgg ggtcaggcct
     6661 gcgttgccgg cagcgtttcc gggcgatcgt gactggttga accaccccga ccggttggaa
     6721 accggcttta cacgctgagg gcgatggctg aggcaatcgc gtgctcgcgg gtgtgcgaga
     6781 tggagagcaa aatgcgcccg atgtctaggg aggcggcgcg ttgcgcgcag gccccatgca
     6841 aagtcaccag gggtttaccg agtgcgtccg gcagggtttc gatatccgtc cagcgtatct
     6901 cgccgcgcca gccggtgccc agcaccttca tgaccgcttc cttggccgcg aaccgccctg
     6961 ccaggcgttc tgcggcattc cggccgccga ggcagtagtt ccgttcgccg gctgtgaaga
     7021 ttcgattcag gaagcgttca ccgtgctcgc gccatacacg tgcaatgcgt tctatctgca
     7081 caagatcaat accatgtgcg acaatcgcca ccatccacca ttcactaacc actaatcact
     7141 aacgactaat cactaacaac tatccactat tcccgcgccg cagcttccaa tatctcgcgc
     7201 aagagaggcc tactcccatg cagccatcgc ggaatgacct tgttctttac gaacatcagt
     7261 ggcgcattgc gaagttgctg ctcttgggcc gcgcgggcgt catccaggat ggcctcggtt
     7321 acggccggat cgttcatcgc atccagaaaa gccagcggct cgaaccctag ttccggcagc
     7381 gcgtcctgca gcgtctgctc gctgaagttc gcctgatttt ccatcagcca gacgtgcatc
     7441 ttccagaaac cgtcctggcc gttgaggcgt cccgcggttt ccgccgctcg ggccgccagg
     7501 caggactggt tatagcgttc ctcgtcgacg gccgggttgc actccgggct gagcgggtag
     7561 tggcggaagg tgtactgcgc ttccgggtgt gaagtaagga actcgcgaat gatggcgtcg
     7621 gcgagcgccg tgttgggttg caggtaatcg ccccaggcga tgatcggcag cgtcgccatt
     7681 ggcgacccat tcgtccaggc ccgcgcggcg ggcgggaagt cgcgcacacg cgactccttc
     7741 cattcgttca cgtagcgctg cggcgccggc gccggctgat cggcttcggc cgtcttggcg
     7801 ggcagattct tcgtcttcag cgtttccacc agtttggtga ggttcagcgg aaccgaccag
     7861 cctttgaact ctcggccatt gataaagacc attggcgtcg tggtcattcc caggtcgaag
     7921 gcctcctgga cgtcggcctg aatgagttgc agcacctcat cgctgttcat gacgcgctcg
     7981 aactcggccg cgtcatagtc aaactccgcc agggccgttc gtagctcgtc gtcggtgaag
     8041 cggccgctgc gatcgaagag ccaatggtgc atctgccaga agccgtcgtt gccgcgcagc
     8101 agcccggcgg cctccgcggc acgggcagcg cggcaggcgt tttcgtgctc gcgctcgctt
     8161 gagaacaggt gattgcactc ggagttcatg gggcattgct tgacggatag cgagacgatg
     8221 tcgctggtcg cgaggagctg cttcagctca atctccagca gattgcagcc tttgcaagtg
     8281 taatccgaga agatgacgag ccgtagcggg acggcctccg ggccgagccg atagcgcccg
     8341 gtaaacccgc ggcgcaaggg ttcggcggtc gaggcagccg cggcagaacc ggccggcgcg
     8401 ctcgcggcag agccggtcgg ctgcgcggga gctggtccga ggggttgacc aacttcggtg
     8461 atgggtggct ttacgctact ttccgctatc ggttgcgagg aaccggcgac gatcgccgcg
     8521 gcagtggctt gtgcatcggc ctcggcgacg gtctgcagct cgcgctgtac gctagtttcg
     8581 agcaccgcca gcgcggctgt cgcaagcacg aacatgccgg ccaacgccgc cgcgggctgc
     8641 cagcgcggcg ccgcggcgcg cggcaaggtc tcgatcagaa tccagaatcc gaggttgctt
     8701 aggtgcgtcg ccaggcaata aggacagggg tagccgccga ggagcatgat cagtacgaac
     8761 ccgactgaga ccagcagtcc caatcgcacc agccagagca gaagcagcgg cgctccgtga
     8821 cggcatatcg aaacccaggc cacgagcagg cctgcgaagt acgccaagcc gaggtacgac
     8881 accggccaat tcacgtatgg cacgctgccc caagcgcttt gactggcctc ggcgcaggga
     8941 ctcccggggc cgcagccggg taagcgcaag ccggcgagat gctccagcgc catcatcgcg
     9001 gccgcgacga tcgcgacggc gaggaagacg attccgatta cgaggcgcgc cgggccggcc
     9061 cggacgcttg ggttcggctg agtctgcgac gtcgcgcgtg ccgcgggcgt cgtagtctgt
     9121 tcgttcattc gacctgctcc tgcactccgg ctttgcgggc gattatcgac ggtcccggaa
     9181 gcgttcgcca gcatgtgtga tggcgtggcg ctagaggatt ttcgaacgag cgctgcaaac
     9241 ctgcgcccgg cagggtttcg tcaacgcgcg atgcgcctat aatgtcggat tcaaaacgcg
     9301 gcaacaggta tggaagatct gtcaaaaatt cgcaacatcg gcatcgtcgc ccacatcgac
     9361 gcgggcaaga cgaccaccac cgagcgcgtg ctctattaca gcggcaccat ccacaagatg
     9421 ggcgaggtcg attacggcac caccgtcacc gactttgacg tggaagagca gcagcgcggg
     9481 atcaccattt acagcgcggc cgtttcgttc caatggcgcg attgccgcgt caacctgatc
     9541 gacacgcctg gccatgtgga tttcaccgcg gaggtcgagc gcagcttgcg ggtgctcgac
     9601 ggagcggtga ttatcttcga cgccaaggaa ggggtggagg cgcaatccga gaccgtgtgg
     9661 cgccaggcgg acaagtacaa agtgccgcgc ctatgcttca tgaacaagat ggacaaaacc
     9721 ggcgcggatt tcgaacacgc cgtcgccacc gtccgcgatc gcctggccgg caacccgatc
     9781 gcgatccagc tcccggtcgg gcgcgaaaat accttccgtg gccacattga tttgatacgc
     9841 atgaaggccg tgttctatca cgggggcgac ggccgcaact ttgaactggt ggatattccc
     9901 gccgatctcg agagcgaggc tcggcaagcc cggcgtgagc ttgaagagaa actggccgag
     9961 ctcgacgacc acctgatgga aaagtacgtc gagggacgcc cgttcgagga aggcgagatg
    10021 cggaccgccc tccgggccgg caccatcagc ctgcgctgcc agccggtgct gtgcggctcg
    10081 tcgctgcgtt acatgggcgt gcaggccctg ctggacgcgg taaccgacta cctgccgtcg
    10141 ccgctggacg tcccgccgat tagcgggttc gacccggaca agcccagcaa aatcgttcag
    10201 cgcaaatgcg atccgggcga accgctggcg gccctggttt tcaaagtcat tgccgagccg
    10261 cacgccgatc tgcacttcct gcggatctac tccggcgtcc ttaaggccag ctcgcgcgtc
    10321 gtcaacgtcg gccgtaagaa gaaggagaac gtgccgcagc ttttccatct gttcgccaag
    10381 cggcgcgaga agatcgagcg cgccatgtgc ggcgacattg tcgcggtcgt cggcctcaag
    10441 gagacgctca ccggcgatac gctgtgcgac agccgcgcgc cgatcctgct cgaacgcatc
    10501 gaatttccgg aaacggtcat cagcatgtcg atcgagccga agtcttcagc cgatcgcgac
    10561 aagcttgtga acgcgctgaa gatgctctcg cgtagcgatc caacgttcga cttcaccgtt
    10621 gagcccgaaa ccggccagac gctcattcac ggcatgggcg agctgcacct ggaggtcatg
    10681 tgccaccggc tcgagcgcga catgaacgtt gacgtgcgcg tcggcaagcc gcgtgtggcc
    10741 tatcgcgaga cgatcacgca agcggctgaa gccgaaggcc gcatcgcgcg acaggtcggc
    10801 gggcgagccc agtttgcggt tgtgcggctg cgggtcgagc cattccagcc ggcgcccggg
    10861 caagagcatt tccgcttcga aaatgcggcg cctgcggatg agttgcgacc gcagtttgcg
    10921 gcggcggttg agcagggggt gcgcggcgca gcccacagcg gcgcgctggg tgggtatccg
    10981 ctgatcaacg tgaaggtcac gctgctgggc gggcaggagc atgccgagga gtcgaccgag
    11041 gtcgcgttcg agtcggccgg ggcgctggcc gtgcagcgcg cattggagca ggccggcccg
    11101 gttctgctcg aacccatcat gcgcgtggag gtggtcacac cggaggagta cttcggcgcg
    11161 atcaacggcg atctgatgtc gcgccgcgcc acgattaccg ggacggcgat gcgtgcccgc
    11221 gaccgcgtga tttcggccga ggtgccgttg gccaccatgt tcggctacgc cacacagatc
    11281 cgctcgctat ctcaggggcg tgcgtcgtac tcgatggaac cgtaccgcta cgaaccgctg
    11341 ccgcaggaac tggtcaagaa ggttttgggg atatagacgc gcgctataga agcgctctta
    11401 cgcgtgagac tgcttgaggc gccacgcgtg tgccactgct tgaagccgaa gcttcaagca
    11461 gtgcatccaa cacacacgcc cggtttcgtt actcggcttc cggggcggca ccccccggtt
    11521 ttgctacggt cgcgccgcgg ttccgctatc caccggtcag caccagcacg aacgggtcaa
    11581 tgtcgaaggc gttaaccgta ccgtcgccgt ttacgtcggc gagcatgtgg ttgcacccgg
    11641 ggtaggcgct accgtaggcg ccggggtcgg tcagcgccag tacgaacgga tcaatgtcga
    11701 aggcattgat caagccgtcg caattcaggt cgcccggctg ccaggtgtcg tcgcaattca
    11761 gcgtggcaat cgagaaatcg tcgagcgcgg cctcgaccag ggagccgtcg ccttcgtccg
    11821 aggcgatgaa gcgcagccgt acctggtccg tcagcgctac gaaatcctga acattgaact
    11881 cgtggtagaa ccagccgggc gaagtctccg ggccggtggg tccgacagtt tcggcgttga
    11941 cccaggtgct gccgccgtcg tcgctgatgt ctacaacgaa cacgtcggtg tgcggtgtgg
    12001 cgccttgatc gttggagtac cagcgccagt agccgatgac ggcataggtc gaacctgaca
    12061 agtcatagtt gggcgacttg agcgtagttt tgccgccatc cacatcgtag ctgccgaggg
    12121 cgccgccgcg cgaatcagtt acccagcaca ttgtacctgg ggccggtgtg tgatctgagc
    12181 cgggctgggc cgcagtggga ttgggatcgg cgcgatccca aatacccgcg gaggcgtcgt
    12241 cgcctgtatc gccgacagtc cagccgctgg gggcttcgaa atcgtcaaac actattgtcg
    12301 taagcgtgcc cacgccgacg cggtacagtt cagtgggggc gttctccggc agcactacgg
    12361 tggcgccgtc gctgccagtg gcgctgaagt agaactcggg ctgagcgccg cagggcgcgg
    12421 cgggcagcac ggcccggtag aggtcgccgc ccaggctggt cagcgtgacc tctgagaatg
    12481 cgccgggcgc aaagcggtag tacagtcgcg ctgagccggg cacgtacgtt tcgccggcac
    12541 tgctaatgtg cagcgtaagc ggtgtgggtg tcagcgccgg cacaatggaa ggcggatcga
    12601 gcggcaggct cagaacgaga ggcgggagcg tgaaatcgcc cgcggcgaag atggcaacgt
    12661 catcgatgtt ccagccgcag aaagtcagtc cgccgtcagt cgttcccata gtccaacgga
    12721 tgcggacatt gggctgattg tcagccagtg aggagatatc gatgtcctga agtgaccagg
    12781 acgtgtcggc cacgtcctcc gccccgttct gccagacgtt tgtccagctt gtgccgttgg
    12841 tgctgacgcg cacgtaggcg tgatcgtaga tcgacttttc gactcccagc cagcgcgaga
    12901 agcgcagatg tacatccgtc aagccctggc agtcgatcgg gggtgtagtc agatggtact
    12961 ccggcatgtt gttcgtgtag tcgccgttga ggttgtagcc atatacattg cggccggtgt
    13021 ggccggacgt gggatccgga ccgccgcctc cgccgccgct gccggtagga acgccccagg
    13081 cccactggcc ctgcgtggtc cagccgggat tagtgttcat attccagcta tagacagtgc
    13141 catagccgac tgccagttca acgccgcgcg tggtatttcc gactgaatcg gtggtattct
    13201 cgaagctgag cgtcgcattg tgcacgccca tgggcagact ggcggcctgt gaattgatgg
    13261 acacggttac cagcgtgctc gccccgccct ccaaagtgcc ggagccgttg gagatgctga
    13321 cccagtcctg cggacaggtg acgctgtaat caatgtcata agtgccgata tttcgcacca
    13381 cgaagacgcc gctcgatatg aacgggccgc cgacatggcc ttcagatcgg aagacgcttt
    13441 cgggcgctac ccgtatgcca gtcaccagtt ccgggcagtt caggccgtgg gcggtgaatc
    13501 ccgcacaaat ttcgttgtag tggggcgagc cgttcccaag gtaaccgtca tcgtcgtcga
    13561 gcgtgaggaa gtcgattaca atgtcgtcgt taattgacgt gcccaagtgc agtaatattg
    13621 aattgaccgt taagctcgac agtatcgaaa gatactcagc cggctcagtc tcaatcagct
    13681 cgttgcgaat gctccagatg caaccggaaa gcaagttgcc gcaatcgtga acttcacttc
    13741 cgcaggtgga acagccgctg gccagatact ggcagtcgtt gtcggcggtg cgcaggcccg
    13801 cgtcgcaatc gccgaagaac ccgtagccca ggttgggatc gtcgacggtc aacacggcga
    13861 tgcagtcgga catgccctcg ccgtacgcgc cctgcccgct gccgccctgg tcaatcacgt
    13921 gatgcccata ctcgtgatcc agcacactct gccagccggt attcgggcaa ccgtcgccgg
    13981 cctgacagaa gttgattgag attccgtcag accaggcgtt gcaggggcag tagccgtccg
    14041 tacgattgac gtagatcgga aagttcgttt ccgtggaaat gccgggaaac tctggattct
    14101 gcgcgaggat ccagtcgcgg acgcgattcg cgctaatgta gcagtttacc tgcgaccgaa
    14161 ccagatcgct gatattctct tcgttgtgtg tgaaagtata cggcacacct ggcgtcacag
    14221 tggccgaaag cgtttcctcc gccccggccc agttgtcgac gttgaagtac gtgccagtca
    14281 tgaaggattg caccgtgacg ggcgaagagc cgctgtgcgg gatcgagaag ttcccgtcgc
    14341 cgtcggcgta gccggttccg ccgcccgaaa ccgagacgcg cgcccaaggc ataactgtga
    14401 tcagctcggg cgagcaaatg tcggccttgg agccctccgt ggccatgccc tgcacgtgcc
    14461 cggtaatggg agcgaagcgg atcaggtctt ccttatagag cacgtttcca gtgactgcat
    14521 cagcaacgta cagccagcgc tccggcttgg cattggggtt gtcgtagtta tcggccgtaa
    14581 aggtgatcgc cagcacgggt tccacttgtg cgtcatctat tccggcccag atcacaacct
    14641 gaatgtcgga ataattcgtc atctccggcg caatctgctc gagccggtcg aagggttttg
    14701 ccgctacgcc gcgatcgacg acaaagtcgc ccagattccg cgcactggac gtgaccagca
    14761 ccaccggaaa gccgggctcg ttacgagtga gcaggcgcac ttcgccacgg aacacgggta
    14821 ttccgtcttt ctcctgcgcg aagttcacca gcgtgaactt gttaccgccg cggtctgcat
    14881 ccggcatgag cgggaccgtg ggcaaaccgg taaaattgct gccggcccgc agctcggctg
    14941 acgtcacacc cagcgctggc gcgtgttgct gcaggaaatt tgcggcactc tcctggggac
    15001 tggttccaaa gctcagcggc gccccgaaga cgcggttgac gcgccggccc tcgaatgaaa
    15061 cgcgtgcgtc cggattggcc cgttgcagcg cggcgcgagc tgccctttca tcgacgccgc
    15121 tgaaagccaa tggtacaaca caagacgaca tcgtgactac gaacagtctc cagcgcatgc
    15181 aaatctcctt ggaaatcgct ctctcaacga gggcgggatc cggcaggcgt tccgaattca
    15241 gcatagtgga ctcgcgcgac gaattccaga cataatcgcg cctcggccgg tcagccgcct
    15301 cctcctatcc agaagtcgtc tttttatggg tcgctgtcgg tcgtaggccg gccgcttaac
    15361 agtgtagagt gtagtccaga agttgcgcgg cgccggttga ccaaatttcg ccgccgcgag
    15421 gggcgctggt cccctcacag aatcagcatg gcgtccccat agctgaagaa ccggtaccgc
    15481 tgagtgatgg cctcattgta ggctgcgcgg atttgctctg gattggcgaa cgccatcacc
    15541 agcgccagca atgtgcttcc aggcagatgg aaattggtga tgagcacatc gacgtgccgg
    15601 aagctataag gcgggtaaat gaatatgtcg gtccagccct cctggcccgt gccttccaaa
    15661 gtgcttagcg attcgagcac ccttacggcc gttgtgccaa cggccacgac gcgtcctcca
    15721 gcagagcgga ccgcgcgaat ggttgcgagt gcctgaccct gtatatcaaa ccattccgag
    15781 tgcatcttgt gctcggcaag gtcttcgacg tcgatcggcg cgaacgtccc caggccgacg
    15841 tgcagtgtca cacgcgccac tctgatcccg cgctgttcga gggttcgcag caacgccggc
    15901 gtgaagtgca gccccgcggt cggtgcggcc accgcgcccg gccgctcggc atacacggtt
    15961 tggtaccttt cgcgatctga ggcatcgggc gtaccggttc ggccggcaga gtcggggcgg
    16021 atgtacggcg gcaagggcgt ctgaccgatg ctctccagca gctccagcca cggcactgcg
    16081 ggttcgggcc ggacgagcca ctgcccgcgc gggcgccgct cgatgagcac caattccacg
    16141 gcgcggcccg cgcatttcag ccgttcgccg gtagccaggc gggcggaggg cttcagcagg
    16201 acactccagc cggcatcgtc cccgtgcaag aacagccctt cgatcaggcc gccggtttgt
    16261 cgccggcaga agaaccgggc ggggatcacg cgcgtatcgt tcacgacgag cacatcgcca
    16321 gggttgagaa acgcgcatac atccgaaaac cgccggtgct gcacgcggcc ggtgcggcgt
    16381 tccagtacca gcaggcgcga agcgtcgcgg ggctcggcgg gccgttgcgc aattaactcc
    16441 ggcggcaaat cgtattgaag gtcctggatt cgcatgccgt tggcgtagta ctctatggtt
    16501 agttcaaggt ccgccgggcg gagcggttct ccaatcgccg gctcaacccg gcctttacaa
    16561 taggcacgct tggtggccgt cgccagccat ttcgcccttg cgggacggtg gccgtgtcca
    16621 ggaccaaatg ttgaggcgtc gcatggctga ttggggcgag gcggaagtcc gtggtcgttg
    16681 cggttgcggc aggcgctatc gcatcctgca cgcaaagccc gaccaggtcg tgacgtgccc
    16741 gaattgcggg cggggcatta tcgtcacccg ggccgatctc aaggcggccg gcgccggcga
    16801 gatgctcatt ccgctccaac ccgaacaggt cgaggtacgg gaagcactgc tggtggatga
    16861 agtggagttg cggcctgcgc cggccggcag tggaccgggt ctgacgggcc ggcaggtgca
    16921 cacgcacgaa gtggcgctgt tggccgaagc gttggagccg agccgccaaa ttgttcatgg
    16981 cgccggggcg tattcgcccg gtgcgccacg cagccagccc gcagcgggtg cgcccgtgcg
    17041 cccggccgcg tttctgctgg acatgctggc gagtttttgg tttgccgggc ggccgcgaaa
    17101 tgcgctggta ttggcaatta cagcccttgt ctttacggtg ccgctgctga ttctgggccg
    17161 accggtaatc ggctacgtcg tgttcctctt gaccgcgtta ctggaatttt tgctgtttct
    17221 ccacgcgctg cagttcttct ggcacgtcat gacagagact gccatgggcg aggatgatct
    17281 tccggtggtg ccgcgcgggt gggactggat cgaggacgcc ctcaagccgc tggggtggct
    17341 gctgctgatc acgctcttgt gcagcgtgcc ggccattgcg gccggatggt atgctccgcc
    17401 cggtcagtac cgcgagccgc tcatttgggc ggcagtgctg cttggcacat ttgtgtggcc
    17461 gatcgcgctg ttgtcagtcg ccttggccaa tgggcgggtg ctgctgcggc cggacttgct
    17521 ggtgcgctcc gtgtggggga tcgggccggc ctacatcgcg gcctggtttc tggtgctagc
    17581 gcttgtggca gcgatcgtga tcttcgttgg ggtcggtccg gccgcgatgg gatggccggc
    17641 ggtagtggcg acggtttacc cgattgccac gggcgcgatg gtcttctacc tggcgtacgt
    17701 tctgttccgg acgctgggcc tgctctttcg gcatttcggc gggcgcttgc cctggcgagt
    17761 ctgagcttga cgccctgcct tcctgggctt cctcagcccg acatcgtggc caggttgaac
    17821 agcatgctga agcacacaca ggctgtcagt aacgcggata ggaccagagc cacgataccg
    17881 ggcacgttgt cgcgccggca ctgaaccacg ctcgtgatcg acagggcgag tgcgataacg
    17941 ataaaaagct ccgctgcgca gctcaggccg acaatctccg tccgggattg tacgccacgc
    18001 gcgatttctt ccaattcctc gagctggctc cccgtagcct gccccggttg cgaggctgac
    18061 acccgcgcta tttctttcag tgccggccaa gacaggacga gcagtgccac catcgatatt
    18121 ccggccaagg ccaggcagat gagtgcgtat cggccgtact tccgccccgt ctgttcgcgt
    18181 gcgtcaagag ggcgggcggt ttcgggtggc ggtggtgacc agccctcttc tgcctgccca
    18241 gatgaggccg tggggcgggc ggcggcaatg ggctgttcct ccaacgagga agccagcggg
    18301 accgccacca cacggttgca atagggacag gccgccattt tcccggcgtg ctcatcgtca
    18361 acttcgatcg gctgcccaca gccgctacag cggaattgaa tcggcatgat gcacttccag
    18421 aacaacaacc tggccaggcg ttcctcgccc ggccaccgcg ccaaccgtac tgcatgatac
    18481 ctgccggccc aaaaccgtgc cacgcggcgg cgagacctgg gtgtacaccg tccgagcagg
    18541 gtcgtagcat ggaatcgttg acagagccgg agactgaggc cggacgcaag caatccgcta
    18601 tgtcaggtta ccagcgatgg accagaacgc accggtatta atcggcgttg acgggggcgc
    18661 gagcgaggtc aaagcgcacg ccgtagcctg tgatgatttg agccggccca gcgcgttcgt
    18721 cctgcggccc gaatcggctg cgcgcgttta cgcacgtgat ccggggttcg cgccgctgcc
    18781 ggtggcggaa caaatagcgc agcatcgctc gggccgttgc gacatctcgc ccgccgagcg
    18841 gcagcagggc gaactgtggc tccaggccgc ggcggacgcc atctgcgacg ttgcgcgtca
    18901 gtgcggcagc cggcgggcgc tgattgggat cggcatgccg gggctgaaga cggccgacgc
    18961 gcggggcatc tgcgtcatca acaacggccc gcggattccg gattacctgg atgaactaga
    19021 gcggcgcctc gccgccgacg gcctcgaatt ggcggctccg atcgccgaac tcggaagcga
    19081 cgcggactac tgcggcctgg gcgaagaata cgcggccgac ggcctctttc gcgacgtgca
    19141 gaacgcctat tacatcggct gcggcacggg catcgcggac gcgttgaagc tcggggggcg
    19201 gctggtgccg tttgaccagg cgaagtcctg gattctcaaa tcttggcaaa tacccagcgc
    19261 cctgggaccg acgttcgagc aactcatctc ggtcgccgcg ctgaaccggg tctacgccgc
    19321 gttgatcggc gattcggggc cgtcggcgta tccggagcgg gctgccgcgg ccggagaacc
    19381 gcgagcttgc atgtggatgg cgactgccgg gctggtactg gccgagctga ttttcgaacg
    19441 gctgtggacg atcaagaacg gacgtgcgca agcggcgcat cgcggcgaaa actatctgca
    19501 gctcaatccg gaacacgaat tccgcgggac ggtcttgcag cgcgtcgtca tcggtcagcg
    19561 cctcggacag atatgggccg atccagcctt tggagacatc ttgcgctgca agctcgaggt
    19621 agcgcttgca gcgcggttgg ttgagtgcgg cgatgcggat ctgatgtcgg cctgtctcgt
    19681 agccacgccg caatttcgca tagtgccgcc gcacctactg aggccctcgc ggttgcgcgc
    19741 cgcgccggcg ctcggcgcgg cggtcgcggc tctgcgggcg tcgggcgagt tgggtaaggg
    19801 cacgtgagga cagttgcgca accggggttt ctggctgcgc gcggcgcact ttacaatgcg
    19861 gcccgattgg ggtttgatgc acggtagatt tgcctctgga gagtcaaagg aggagaccct
    19921 gtgattcgtt cagctcttgt gatcgtggga gcggccgtcg cattgtcgct tactgcctgt
    19981 cagaaaccgc aggcccagcc ggaagctcac gtggaattca cccccgctcc cacactggac
    20041 cagtcggaac gcacgccggt gcaaccgctg gacgcctacg tggccacgcc gccgccggtt
    20101 tacaatccgg agccggcgta caccccgccg ccggagccgg ttatcaagcc ggccgacaag
    20161 ccggtgaggc ccgcgcatcg cgagcgcgcc cgcacgtacg tggtaaagaa gggcgacact
    20221 ctgagcgaga tttcgcaaaa gttctacaac acgcctaatc gctggaggca gattctcaag
    20281 gctaaccccc acgtaaagag cccgaagaaa ctccaggtcg gcacgaagct ggtcattccg
    20341 tagcggttcg taatcatcgt cgccccggaa gggccgtgcg cccgccgccg ggcacgtgac
    20401 tacacccagc cgcgcttcaa cggggcgcgg ttgccggtgt attattcaag tctaatgtac
    20461 ctgctgcgcc ccgacagtcc ggtgcagtac ctcaagggcg taggcccgca gcgcgccgcc
    20521 tggttcgagc aggccggcat caagtccatc accgatctga tcgagtactt ccccttccgc
    20581 tacgagttcg acaccggttt ggtggacatc gccgacctcc agccgcggac cactgccacg
    20641 gtgcgcggca cggtcctgta cacgcacggc cgcgggccat acggctcgta cacggccgag
    20701 atcgacgatg gcacgcaaac ctgcacgctg cgctggtttc agagcaagta cgtaccgcgc
    20761 gggctgtgcg tcggagcgtc ggtcatcgcc agtggtgacg tgcaggaatt caacgaccgg
    20821 ctcgaaatcg ttcagccgcg cgtgcaggta ttcccgccga acacgccggt cgaagtcccg
    20881 cggcgcggcg ctcgaatggt gggcgtgtac cgcgcggccg ggaagctcac ctcgcgcgtg
    20941 gtgcagcggg cggtcgaggc ggcgctttcc gcgccgcagc ttccggtgga agagttcctg
    21001 cccgcggacc ttttaaagaa gcacaatttc ccagcgcgcg gcgaagcgat tcggcaaatg
    21061 cactcgccgc ggggcgatga acaactgcgc cgggcgcgcg agcgactggc gtatgaagaa
    21121 tttctgctgc tggaactggc gatggccctg cggcggcagc gcacggtcgt gttgcagccc
    21181 ggacagaagc tgcacgtgac gcccgaaatc gaccgccgca ttcgcgcccg cttcccgttc
    21241 gaattgacgt cctcgcagaa cgaggtcgtg agcgaaatcg ccgccgacct gagcagcggg
    21301 cggccgatga cgcgcctgct gcagggcgac gtgggcagtg gcaagacggt agtgtcgctg
    21361 tacgcgtgcc tggtagcggt cgcgaaccgc aagcaggccg cgatcatggc gccgacggaa
    21421 attctggccg agcagcattt tcgaaacatc gagcaatatc tggccggcag ccgggtgcgc
    21481 tacgtactgc tgcgcggcgg cctgtcgcgg gcagagcgca cgaagatttt ggccagcatt
    21541 gaagccggcg agctcgacct ggtagtcggc acacaggcgt taattcagga agatgtgagc
    21601 ttcaagaacc tggccgtcgt ggttgtcgac gagcagcaca aattcggtgt catgcagcgg
    21661 gcccgcattc gcaccaaggg gccgatgccg cattacctgg tcatgacggc cacaccgatc
    21721 ccgcgcacgc tggctatgac cg
//
LOCUS       DAOSYS010000031        21528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_1994_length_21528_cov_5.015135, whole genome shotgun sequence.
ACCESSION   DAOSYS010000031 DAOSYS010000000
VERSION     DAOSYS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 21528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21528
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1994_length_21528_cov_5.015135"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..275
                     /locus_tag="PLU99_03290"
     CDS             24..275
                     /locus_tag="PLU99_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOO16113.1"
                     /translation="MPVKITIELPEEAFSATRSTPEEFAQKLRIAAAVKWYEVGMLSQ
                     GKAAAIAGLNRHEFLQALANLGVSPFQATITDLEAEMGR"
     gene            272..757
                     /locus_tag="PLU99_03295"
     CDS             272..757
                     /locus_tag="PLU99_03295"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOO16114.1"
                     /translation="MSRRWVLNASPIMALSATGHVSLVTELSEQAVVPSGVAEEILRG
                     PADSPAVQWLNGEGGRLVQAVDGLDPAVLAWDLGRCETEVLTWARRHPEFEAVLDGRA
                     ERVCAAAFNVPVRCTLGIILLAKRLGRIPAVGPVLAALEEAGFPVDPTLRAQIIQHAG
                     D"
     gene            758..1762
                     /locus_tag="PLU99_03300"
     CDS             758..1762
                     /locus_tag="PLU99_03300"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOO16115.1"
                     /translation="MNSEIRTAVPADVRSAILQAGRIALIGHVTPDADCIASMAAAWL
                     GLRELGKEVHALLPDGTVSRRMQFLVRRAGLTPATLDEVKSCELALVLDTAKASRVNL
                     PGKLEALPGLAVANVDHHATNAGFGRWNWVVPSASSTSELMYTLLRSLGCQVGPNIAT
                     LLYAGMHSDTQGFSLSNTAAESLNVAHELAACGAAIADVCEQLHRSHSRGEFELLKLV
                     YAHTQVSEDGRLAWSCASHSEIAGAGCTASDIDDQVEVVRSIDGVKVAILFTEGVRGK
                     VRINFRGEKGVAVLNLAARFGGGGHTESAGAILDGTVEEIMGRVIPAALEFVRDLPPG
                     "
     gene            complement(1771..2511)
                     /locus_tag="PLU99_03305"
     CDS             complement(1771..2511)
                     /locus_tag="PLU99_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16116.1"
                     /translation="MDRPPLKPLSLTITFPCYNEEANVERVTRAALAVAQKVADDYEI
                     VIVNDGSRDRTGEIADRLAAENPHVRAVHNRPNQGYGGALARGFKEARKNWIFYTDGD
                     GQFDLNELPTILPLLEKYDIVSCYRLNRQDSAMRKLNAWLWSTLVNILFRLRLRDIDC
                     AFKIYPRTFIDQIELWSRGALIDTEMLAKARNLGYSIVQVGVHHYPRTAGQQTGANIK
                     VIARAFKELFKLRGRIKREGRDQQGRGA"
     gene            complement(2565..3746)
                     /locus_tag="PLU99_03310"
     CDS             complement(2565..3746)
                     /locus_tag="PLU99_03310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO16117.1"
                     /translation="MRTSRVLLLVTDLERGGTPLRLARLASGLRERGLEVHVGCLARP
                     GPVSRDLDAAGIPNFACNACRVRDVGALWRLTRHVRRIRPDVIHSTLTHANVAARIVG
                     LLCGIPVVTSTATIEVERKWHAWAERLTRALDSGHIVNSEAVADHVRRVFRYPRRQVF
                     VIPPSIDLPGSRAAVGAGVAPPAAGVAPPGAGDSDRVVVRQQLGITSDEFAVLWAGRL
                     DPVKRIDLVLECAQRLRQEPFRFLIAGDGPERGQVDQALRRREAGQLQWLGWRDDLAA
                     VMSAADLLLFPSRTEGVPNVILQAMAGGLPVVCSDIPTLRELSGDGRRLMLVSQGDAD
                     SFMAAIHLLRDHPQRRREQSERARLWAAANLDPQRTIAETIRAYETVLAGRRRSPAAG
                     G"
     gene            complement(3783..7214)
                     /locus_tag="PLU99_03315"
     CDS             complement(3783..7214)
                     /locus_tag="PLU99_03315"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HOO16118.1"
                     /translation="MSASSQMFGRSVGLFGGITGAMSALSCIALLLTVGSAAAQQCDW
                     LPGHGVAGTAGQVNAIVTWDPDGAGPASEVLVVGGQLTAAGDTITGGVAAWDGSAWQA
                     LGFAGGDGDTAVNALAVFDGQLIAAGYLPLGGGLQHGVARWDGAAWQLLGSGVEGDAF
                     TAVNALAVHNGQLVAGGSVRSAAGAEYGCVARWNGIAWRPMGSPLAGEFTMFTSATVK
                     ALCVYADELIAGGRFTVSGIAPGHAIARWDGSDWQTVGAGMGDATDLVNALALYNGEL
                     IAGGSFMIPGSPGCHNIARWNGSAWQALESGLDNEVAALTAYGSELVAGGYFSAAGAV
                     PCENIARWNGTAWQALGSGIASAVVYSSVHGLGVYNGELIVGGSFLLAGGVACSNVAR
                     WDGSAWNSLGAGTDALWLSDLLEYGGDLIAGGALVSGGGSCIGVGRWNEAAGTWQALG
                     SGVNVAKRLILYDGELVAAGSFTAGDGTMYHGVARWAGSAWQALGPWAADAGSPNIEA
                     LAVYNGDLIAGGMFTSAAGVPCNNVARWNAAAGAWQPLGAGIDGIVGALTVYRGELIA
                     AGAFATAGGTPCSSIARWNGTAWQPLGAGTDGIVAALAEYDGELIAGGYFTSAGGVAC
                     NNIARWDGSTWRPLGAGIGSSGYSYVSALGVHNGVLIVGGLFATADGAQWIARWNGLT
                     WQTLGLGMDNLPYALLSYQGELVAAGAFTAAGGHVSCTWGRYSGEWPAAPNNVSASDG
                     LFCDKVRVTWGEVPGATGYKVYRSATGSVADGLLLGTSQSGAFDDVRAAGRTVWYFVV
                     ASNACGDGPCSAIDGGFVQCGELPTWYRDSDGDGYGDPGTTTQAARQPPGFVANDDDC
                     DDGCATCHPGAQEVCDGRDNDCDGWIDAGAIGPTWYRDADGDGYGNPDVTVRACSRPA
                     GYVENGDDCNDTIPAVNPGVVEICNNGINDDCDGLIDCDDPDCAWDLGCAELIADAGP
                     DLNLSVASAAEGANVMLDGSRCSGNIVEYTWTENGQWLAYGRTAVVLLRPGTHDIVLT
                     IRDAHGRTASDTLRVSVNLDDGGDEDLDGVINAEDDCPGTPAEEAVDANGCSCSQLDT
                     DGDETSDCDDLCPTIPGSCPNGCPPGPSGLCGSTGCGLIGLALAGMWLSRRRNRV"
     gene            7935..11234
                     /locus_tag="PLU99_03320"
     CDS             7935..11234
                     /locus_tag="PLU99_03320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOO16119.1"
                     /translation="MSNGGSYDPHAVEPEIYKKWEDGGYFHAVPNPNKRPFVIDIPLP
                     NVTGALHLGHALNNTLQDILIRHARMRGFEALWMPGTDHAGIATQAVVERRLKEEQNL
                     TRHELGREGLVKRIWEWKERFGGQILMQLRKMGCSLDWQRTRFTLDEVCARAVYEVFF
                     RWFKEGLIYRGVRLVNWDAFLQTAVADDEIVHETVKGSLWHYRYPILEGIKGSRDQGI
                     KTLSDETVRAALARAVAADAKMGRDYLTIATTRPETMLGDTAVAVHPTDERYQHLIGR
                     YCLLPLMNRPIPIIADAILVKKEFGTGCVKVTPGHDPNDYAFGQRHPEAAPINILTPD
                     GKINANGRTQRDGGTPAEPSSAAGLFYDYVGLDRYEARKRVVADLEALGLLEKIEPYE
                     TEVGHSDRSKTPIEPMLSEQWFVKMGDLAERAMEAVRDGRVRFFPARYAKTYLDWLGE
                     KRDWCISRQLWWGHRIPVWTRVLDSRQQFEEFKAVLKLVARYRSFQELVAIEAYGMGS
                     QKPIELNELSETGISGFDERVLLMVVLRHPDRSELVRLFRHYYTLTPAQAQFDWPQYT
                     DEAWRRDPEQFVDKLFGDLEQLQRDPDVLDTWFSSALWPFSTLGWPHDVRDQASKGSS
                     DQDTAIGAQPLDPSIPRSLDPSPEDFDYFYPTSTLVTGRGIITLWVARMVMTGLHFPG
                     RVPFQHVHIHPIILDGQGRIMSKSLGNGVDPLDIITLYGTDALRFTMAQMDTETQDVR
                     MPVKPVKMPDGRTVNSSEKFELGRNFCNKIWQAATGFVLQNLNGAQPRPLTSADLALE
                     DRWILSRLAATIAEVDRRLEHYQVSEVANLLYNFFWSEFCDWYLELVKPRLFGRSAAG
                     EMVQHTDASSQVARQVLAWVLDQSLRLLHPIMPFVTEALWAKLNAAAPQRGITRLIEG
                     EPMLIRAAWPDAAAWPREPEIEDDLAALQNVIRALRDSLAWINTTRAAAKTPAIGKLP
                     KALIRAKDQLVAGLQEQTAVICRLGRCEAIEIGPDLTKPPESATKVFPGLEIYVPIAG
                     LADLDSERKRLAKERDELTGHLQRLEAKLANGEFVSKAPPAVVEKERARLVELKEKLA
                     AIGHHLAELGE"
     gene            complement(11406..>11918)
                     /locus_tag="PLU99_03325"
     CDS             complement(11406..>11918)
                     /locus_tag="PLU99_03325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO16120.1"
                     /translation="RRGERGLATAVIRGLREARGDLLVSLDADLSHPPESIPALLDAL
                     RDPRVNMAIGSRFVPGGRVDLHWPLHRRLNSLVARLLARPLTPVRDMMSGFFAVRRKD
                     LRFEGLDPIGYKIALELIVRHGWKNVVEIPITFSDRAAGKTKLSVAEQLRYLRHLARL
                     YRFKLTGRKR"
     assembly_gap    11919..12018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12019..12623)
                     /locus_tag="PLU99_03330"
     CDS             complement(<12019..12623)
                     /locus_tag="PLU99_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16121.1"
                     /translation="MLLSVVIPVYNEKKTLFEIIRRVLESPIDLERELVIVDDCSTDG
                     TRDLYPQLEQKFPNARFRVVLQPVNQGKGAALRAGFKEATGDIILIQDADLEYDPNDY
                     PKLLAPILKDKADVVYGSRFAGGEEHRVLYFWHLVGNRFLTLLSNVFTNLNLTDMETC
                     YKVFRAPVIKNMTLRCNRFGFEPEVTAKIARGGWRVYEVGI"
     gene            complement(12746..13249)
                     /locus_tag="PLU99_03335"
     CDS             complement(12746..13249)
                     /locus_tag="PLU99_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16122.1"
                     /translation="MKRHQLIQTVQRYAQLTLRQLAERLPAEAAARPRCPVTRYLLGC
                     CCLDQGRAALGVRHLMVAYHAEPRLESAALLVFAGLSWVGQREAALLPVLLATWDEFR
                     RPQFDRTWPERLLLDAFAAPEPGLGQAPLLARRLWRLPLTTLRDQIREAMPSPAAALY
                     PLLAVPV"
     gene            complement(13364..13960)
                     /locus_tag="PLU99_03340"
     CDS             complement(13364..13960)
                     /locus_tag="PLU99_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16123.1"
                     /translation="MRSFCSALLFLSCLGLPLAPGDVIVYYTQDAGGANPSPLNGLAA
                     QALFDIDGKHLSILLKNTSTGVPAGFDAASSLLVSLGMNLPGADILSGDAAIIGPDAK
                     GLGAWSDRTAGASVGEEWLWTNDYGGDLMEGWRHVLSTSQGQGGGTVTRFDGNSGTVD
                     GPFGGIAAAPAILSIPPNRPAVSDAILFELCRVLSRVF"
     gene            complement(14182..14922)
                     /locus_tag="PLU99_03345"
     CDS             complement(14182..14922)
                     /locus_tag="PLU99_03345"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOO16124.1"
                     /translation="MRRPAIGITCSFQQTDPPNPRLQTRLNAAYSDAVFAAGGLPRPI
                     ALPAEPEPSLLDDLLSGLDGVIFSGGLDLDPQRYGQTRHPKAELMHERRDRFELALFR
                     RADELRLPILAICLGYQVAHVARGGRLIQHLDDVPRTPAIAHHLPHGQAFHEVTLAPG
                     SRLAQLIGTTRLEVNSRHHQAVDPDYPGRGLRPVAFAPDGVLEGAEDCENQYLLAVQW
                     HPEDLIDRREHLCLFADLVERAAEKRSS"
     gene            15174..17438
                     /gene="bamA"
                     /locus_tag="PLU99_03350"
     CDS             15174..17438
                     /gene="bamA"
                     /locus_tag="PLU99_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOO16125.1"
                     /translation="MARAQEAVEGMVIKRVEVCGLETISEAYVRRVIKTREGQPFRRA
                     QVEEDVRELLRTRRFLNAFAATAVEENQAVVVFTVQEKPELVSVELEGNKAFPDDQLF
                     ALTPAAGDVIDMYEVRRGQDEILRKYREAGYYYVEVTFDERALQYEGRLIYRINEGPK
                     VRVRQILFEGNRSFPDWRLRSKVQTRTALWIFRVGAFDAETAERDAIEIQRFYRDEGF
                     LDARAGYRLEFDEVTRADLTVVFVIEEGQRYCIQDIVVEGNTVFSAEHIRSCMQLEPG
                     YLLRTEAVEEDRKRVQDLYGEVGYVDARVAIDHEFLEEPGLVILRVSIIENKQYYFGR
                     IIIRGNQRTKDEVVRRELQFYPGELWNTVEARAAEQRLRETGLFQPDQVRITPLPPED
                     STRPALVEVQEAETVTFLVGIGVSTDAGAIGSLTVENRNFDLFDWPRTWGQFFRGQAF
                     RGDGQRLKFSAEPGTEVSRFRIDFTEPYLLDRPVRLDTSLYLFERGRDGYDEERAGFM
                     FALSKRFPRGLLAGWAVEGAFRVEGVGVDDVDALASRQIKEVRGDNLLTTVKGTIVRD
                     TTDSRVIPSKGYRFTLSWEQAGALGGDFDFGRPTAGIAWYKTLRTDIFDRKSVLALHA
                     DTGMIVGDAPVFERFYAGGFGSLRGFSYRGISPRAGIMKNKVGGDFIFLTGAEYSFPL
                     YGKNFRGVTFLDMGTVEENVTITTWRAAVGVGLRINVDFFGPVPIVLDFGFPIADDDQ
                     DDTQVFNFAFGASF"
     gene            complement(17469..19208)
                     /locus_tag="PLU99_03355"
     CDS             complement(17469..19208)
                     /locus_tag="PLU99_03355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO16126.1"
                     /translation="MDQQTTTGVHPTAAPAAALLEAEASSSVARTAARRHARTWSILL
                     VALLAAGVLWRIVRYALGFPIWGDEAFVAVDFVKRGYAEMIEPLTYGQIVPLVFMWAE
                     LAISRMLGLSEWALRLLPFAAGLASLPLFWRFAQSVLPRRPALLAIGIFAASVYLVRH
                     AAEVKPYATDMLLALGLLMLAWRVCENRGGAGSWAALIVGGAAAVWCSYPVIFVLAAI
                     GTLLAWRLVAADRRKRASRDEPCSGGPTRRTLLLQTLCYAIAVGGSFVLMYFLYGRPH
                     AQAASGLTEINMWERAFPPLALSQIWKLPLWLVLIHTDYMLAYPLGGRAPASAFSLLL
                     VVIGAVRLWGTRRDLLWLLLGPLVFTMLAAAAQKYPYGGSARTSLYMAPAFCLLAGLG
                     LSRVLNYLAALARIMFKWPHGRTCRVYLTCAAVVLLLIAVGGAIGDAVRPYKEGPVQR
                     SRAAVDYVVEQTRPGDRWVVFNATERVDYAPWLGDWRGTGAQFVFDVLRFHPVPLSWA
                     PLPDTVAPDPGGRVWFFAYRGWKVPFPEEQLEGYLGVLTRRLGQPQMQRFFLKEKEDR
                     IEAIDLYLFEPQG"
     gene            19332..19404
                     /locus_tag="PLU99_03360"
     tRNA            19332..19404
                     /locus_tag="PLU99_03360"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19366..19368,aa:Glu,seq:ctc)
     gene            19721..21004
                     /locus_tag="PLU99_03365"
     CDS             19721..21004
                     /locus_tag="PLU99_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOO16127.1"
                     /translation="MRSELRSEWISPQTAVEAPVLSRAAASHPRRERLSFSNPIARGA
                     DPWVIQHAGHYYYCEPVTDTSIAIWKTDCFTRRGTRQIVWKAPRQGWNSNLIWAPELH
                     RLDGRWFIYYAASAAPRENASHRIGVLECLTQDPLGAYIDRGMLYTGDDPLCRSDNRW
                     AIDATVLELHGQRYLLWSGWPDGADLQYLYIAPLKNPWTTAGPRVRLCDNATFDWERV
                     SEDPRQRGLHEAPQVLKHNDRVFVIYSCSASWQETYKLGLLELIGDDPLNPATWRKHP
                     QPVFRSSGDVFGVGHASFVKSPDGLEDWVVYHCKTSRAPGWRRVVCAQRFGWRPDGLP
                     DFGQPVPWRQPLSLPSGSGGVDVLLDGSEDGISYPLRQPRCPRMEAANSARVDLAESR
                     YAWRFGQCPCEAPISFAGAGVIRDCRLCRATSTSA"
     gene            21187..>21528
                     /locus_tag="PLU99_03370"
     CDS             21187..>21528
                     /locus_tag="PLU99_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16128.1"
                     /translation="MSGRSGVLGPSAPGGLFQSFFIGGFECSSHKRRSGRRLDMIAAT
                     DHDRLALYDYTSLNALGIRTVRDGIRWHRIERTPYHYSFAADLPMVRAARQTGTQVVW
                     DICHYGWPDDLS"
BASE COUNT         3890 a         7119 c         6768 g         3651 t
ORIGIN      
        1 ggcatcttcg gctggagaag atcatgccgg tgaagataac gatagagctg cccgaagaag
       61 cgttctccgc gacgcgcagc acgcccgagg aatttgcaca gaagctgcgg atcgccgcgg
      121 cggtcaagtg gtacgaagtc ggcatgctgt cgcagggcaa ggccgcggcc atcgccgggc
      181 tgaaccggca tgagttcctg caagctctgg cgaacctggg cgtatctccc tttcaggcga
      241 ctatcaccga cctcgaggcg gagatggggc ggtgagccgt cgctgggttc tgaatgcctc
      301 tcccattatg gcgcttagcg cgaccggcca cgtatcgctg gtgacagaac taagcgagca
      361 ggccgtggta cccagcggcg tggccgagga gatcctgcgc gggccggccg acagccccgc
      421 ggtgcagtgg ttgaacggcg agggcgggcg gctggtgcaa gccgttgacg gcctcgatcc
      481 cgccgtgctg gcgtgggacc tgggtcgctg cgagacggaa gtgcttacct gggcgcggcg
      541 gcatccggag ttcgaagcgg tgctggacgg ccgagccgag cgggtctgcg cggcggcgtt
      601 caatgttccc gtgcgttgca cgctagggat tattctgttg gcgaagcggc tgggacgaat
      661 cccggcggtc gggccggtgc tggccgcgct cgaggaggca gggttcccgg tcgatcctac
      721 tttgcgtgct cagatcattc agcacgccgg agattaagtg aattccgaaa ttcgcacagc
      781 cgttccggcc gacgtgcgca gcgccatctt gcaggccggg cgtatcgcac tgattggtca
      841 cgtgacgccc gacgcggact gtatcgcctc gatggcggcc gcctggcttg gcttgcgcga
      901 gctaggcaag gaggtgcacg cgctcctgcc ggacggcacg gtttcgcggc ggatgcagtt
      961 tctggttcgc cgggccgggc tgacgccggc cacgctggat gaggtgaaga gttgcgagct
     1021 ggcgctggtg cttgatacgg ccaaggcttc gcgggtgaac ctgccgggca agttggaggc
     1081 gctgccgggg ttggcggtgg cgaacgtcga tcatcatgcc actaacgctg gatttgggcg
     1141 ttggaactgg gtcgtgccct ccgccagcag cacatcggaa ctgatgtaca cgctgctgcg
     1201 cagcctgggc tgccaagtcg gaccgaacat cgcgacgctg ctatacgccg gcatgcacag
     1261 cgacacgcag ggcttttcgc tttcgaatac cgcggccgaa agcctgaacg tcgcccacga
     1321 gctggccgcg tgcggcgccg cgatcgccga cgtctgtgag caattgcacc ggagccacag
     1381 tcgtggtgag ttcgagttgc tcaagctggt ctacgcgcac acgcaggtca gcgaggacgg
     1441 ccgcctggcc tggagctgtg cttcacatag cgagatcgcg ggcgccggct gcacggccag
     1501 tgacatcgac gaccaggtcg aggtggtgcg ctccatcgac ggcgtcaagg tggcgatcct
     1561 gtttaccgag ggcgtgcgcg gcaaggtgcg catcaacttc cgcggcgaga agggcgtggc
     1621 ggtgctgaat ttggcagcca ggttcggcgg cggcggacat acagaaagcg ctggggccat
     1681 tctggacgga acagtggagg agataatggg gcgcgttatc ccggcggcgc tggagtttgt
     1741 gagggatttg ccgcccggtt gacctgctga tcaggcccct ctgccctgct gatcgcgccc
     1801 ctcgcgtttg atccgcccgc gcagcttgaa cagctccttg aacgcgcggg cgatgacctt
     1861 gatattcgct ccggtctgct ggccggccgt tcgcgggtag tggtggacgc cgacctggac
     1921 gattgagtag ccgaggttcc gggctttggc gagcatttcg gtgtcgatga gcgctccgcg
     1981 gctccacaat tcaatttggt cgatgaaggt gcgcgggtaa atcttgaacg cgcaatcgat
     2041 gtcgcgcagc cgcagccgga agagtatgtt caccagggtc gaccacaacc aggcgttcag
     2101 cttgcgcatg gccgaatcct gccgattcag gcggtagcac gacacgatgt cgtatttctc
     2161 cagcagcggc agaattgtgg gcaattcgtt gaggtcgaac tgcccgtcac cgtcggtgta
     2221 gaagatccag tttttccgcg cctccttgaa gccgcgagcc agggccccgc cgtagccctg
     2281 attaggacgg ttgtgcacgg ctcgcacatg cgggttctcg gcggccaggc ggtcggcgat
     2341 ctcgccggta cggtcgcggc tgccgtcgtt aacgatgacg atttcgtagt cgtcagcgac
     2401 cttctgggcg acggcgaggg cggcgcgcgt gacgcgctcg acgttggcct cctcgttgta
     2461 gcaagggaac gtgatcgtga gcgaaagcgg tttcagaggc ggtctgtcca tgtcactcgg
     2521 tccggttcta ggcgccgaat ggtatcagat aacggcgccg actgtcagcc acccgctgct
     2581 ggagaccgcc ggcgccccgc aagtactgtt tcgtacgccc tgatcgtctc cgcgattgtc
     2641 cgttgcggat caaggttcgc cgcggcccat agacgcgccc gctcactctg ctcccggcgc
     2701 cgctgcggat ggtcccgcaa caaatgaatc gccgccataa acgagtcggc atccccctgc
     2761 gatacgagca tcaagcgccg cccgtctccg ctgagttccc gcaaagtggg aatatcgctg
     2821 cacacgaccg gcaacccgcc ggccatggcc tgcagaatca cgtttggcac gccttcagtg
     2881 cgcgacggga acagcagcag gtccgccgcg ctcattaccg ccgcgaggtc atctcgccac
     2941 cccagccact gcaattgtcc ggcctcgcgc cgccgcagcg cctgatccac ctgcccacgt
     3001 tccggcccgt cgccggcaat cagaaagcgg aacggctcct ggcgcagccg ctgggcacat
     3061 tcgagcacga gatcaatgcg cttgaccggg tccagccggc cggcccaaag gactgcgaat
     3121 tcgtcgctgg taatccccag ttgctgccgg acaacaacgc ggtccgaatc gcccgcccca
     3181 ggtggagcga ccccggccgc tggtggagca acgccggcgc caaccgctgc acggctgcct
     3241 ggcaggtcaa tcgagggcgg gatcacaaaa acctgccggc gcgggtagcg aaaaactcgc
     3301 cgcacgtggt cggccaccgc ctcgctgttg acaatgtgcc cggaatccag cgcgcgcgtc
     3361 agccgctcgg cccaggcgtg ccacttgcgc tctacttcaa tggtggccgt gctcgttacg
     3421 accggtattc cgcaaagcaa gcccacaatt cgcgccgcca cattcgcgtg cgtgagcgtt
     3481 gagtgaatta cgtccggccg aattcgccgg acgtgccgcg ttagacgcca tagcgcaccc
     3541 acgtctcgca cgcggcacgc gttacaggcg aaattcggaa ttccggccgc gtccagatct
     3601 cggctaaccg gtcccggccg cgccaggcag ccgacatgta cctccaggcc gcgctcgcgc
     3661 agcccgctgg ccagccgcgc caaacgcagc ggcgtcccgc cacgctccaa gtcggtcacg
     3721 agcaaaagca ctcggctggt ccgcatgcga ccagcgtaat cttcggaggc gtgggcgtaa
     3781 attcaaacgc gattgcgccg gcggctcagc cacattcccg ccaacgccaa cccgatcaac
     3841 ccgcagccgg tgctgccaca caggccggaa gggcccggtg ggcagccgtt cgggcacgac
     3901 cctggaatcg tcgggcaaag gtcgtcacag tcgctcgttt cgtcgccgtc ggtatcaagc
     3961 tggctgcacg aacagccgtt ggcgtcaacc gcttcttcgg ccggcgtgcc ggggcagtcg
     4021 tcctctgcat tgatgacgcc gtccaggtct tcatcgcctc catcatcaag gttgacggaa
     4081 acgcgcagcg tgtcgctggc cgtgcgaccg tgcgcgtctc gaatggtcag cactatgtcg
     4141 tgcgtgccgg gacgcagcag aaccacggct gtccgcccgt aagccagcca ttgtccgttc
     4201 tcggtccagg tgtattcgac gatgtttccg ctgcaccgcg atccgtccag catgacgttg
     4261 gcgccctcgg ccgcactcgc caccgaaagg ttcaggtcag ggccggcgtc ggcgatcaac
     4321 tcggcgcagc ccaagtccca cgcgcagtct ggatcgtcac aatcgatcag gccgtcgcag
     4381 tcgtcgttga ttccgttgtt acatatctcc actacaccgg gatttacagc cggaatggtg
     4441 tcgttgcagt cgtcgccgtt ctccacatag ccggccggcc ggctgcacgc tctgacagtg
     4501 acgtccggat tgccataacc gtctccgtcg gcgtcgcggt accaggtcgg cccgatagcg
     4561 ccggcgtcta tccatccgtc acaatcgttg tcccggccgt cgcagacctc ctgggcgccg
     4621 ggatggcagg tcgcgcagcc atcatcgcaa tcgtcatcgt tggcgacgaa gcccgggggc
     4681 tgccgagcgg cttgcgtcgt tgtgccgggg tcgccgtaac cgtcgccgtc ggagtcgcgg
     4741 taccaggtcg ggagctcccc gcactgcacg aagccgccat caatcgcgct acatgggccg
     4801 tcgccgcagg cattgctggc gaccacgaaa taccagaccg tccggcccgc cgcccgcacg
     4861 tcgtcaaacg cgccggactg gctcgttccc aacagcaggc catcagcgac tgacccggtc
     4921 gcgctgcggt aaaccttgta gccggtggcg ccggggactt caccccaggt cacacgcacc
     4981 ttgtcgcaga acaggccgtc agaggccgag acattgttcg gcgcggccgg ccactcgccc
     5041 gaatatcgtc cccacgtaca agacacatgg ccgcccgcgg ccgtgaaggc gccggcggcg
     5101 acgagttcgc cctggtagct caatagcgca tacgggagat tatccatgcc taagcccagc
     5161 gtttgccagg tcaagccatt ccagcgggcg atccattgcg cgccgtccgc agttgcgaaa
     5221 agacctccca caatcaacac cccgttgtgc acacccagcg cactgacata ggaatagccg
     5281 ctgctgccga tccccgcgcc taacgggcgc caggtgctgc cgtcccagcg agcgatattg
     5341 ttgcacgcca ccccgccggc gctggtgaag taacctccgg ctatcaactc gccgtcgtat
     5401 tcggctagcg ccgctactat gccgtcggtt cccgctccca gcggttgcca ggccgtgccg
     5461 ttccagcgcg caatgctgga gcacggcgtg ccgccggcag tcgcgaaggc accggccgca
     5521 atcagttcgc cgcggtacac tgtcagggcc cctactattc cgtcgatccc ggcccccaac
     5581 ggctgccagg cgccggctgc ggcgttccag cgggctacgt tattgcacgg tacgccggcg
     5641 gcgctggtga acatgccgcc cgcgatcaag tcgccgttgt agaccgccaa ggcttcaata
     5701 ttcggggaac cggcgtcggc ggcccacggc cccagcgcct gccaggcgct tccggcccaa
     5761 cgcgcgacac cgtggtacat cgtgccatcg ccggccgtga agctgcccgc cgcgaccaac
     5821 tcgccgtcgt agagtatcag gcgttttgcg acgttcactc cggaacccag ggcctgccac
     5881 gtaccggcgg cttcattcca gcgtcccacg ccgatgcacg acccgccacc ggacaccaga
     5941 gcgccgcccg cgatcaagtc gccgccatac tccagcaggt cggatagcca taaggcgtcc
     6001 gtcccggccc ccaacgaatt ccacgcgctg ccgtcccagc gggcgacatt gctacatgcg
     6061 acgccgccgg ccaacagaaa gctgccgccg acaatcagtt cgccgttgta tacgccaaga
     6121 ccatgaacgc tactgtagac aacggccgat gcaataccgc tgcccagcgc ctgccacgca
     6181 gtgccgttcc aacgcgcgat gttctcgcac ggcacagcgc cggcggcact gaagtagccc
     6241 ccggcgacta actcgctgcc atatgcagtc agcgcggcga cttcgttgtc caatcccgat
     6301 tccaacgcct gccaggcgct gccgttccag cgggcgatgt tgtggcagcc cggactgccg
     6361 ggaatcatga agctgccgcc cgcgatcaac tcgccgttgt aaagggccaa ggcgttcacg
     6421 aggtcagtgg cgtcgcccat gccggccccc accgtctgcc agtcgctgcc gtcccagcga
     6481 gcgatggcgt gccccggcgc aatcccgcta accgtaaacc tgccgcctgc gatcagctcg
     6541 tcagcgtata cgcagagcgc tttcacggtg gcgctggtaa acatcgtgaa ttcacccgcc
     6601 agcggcgagc ccatcggccg ccaggcgata ccgttccagc gcgccacgca gccgtactcg
     6661 gcgcccgcgg cgctcctgac gctgccgccc gcgaccagtt gaccattgtg gacggccagc
     6721 gcgttgacgg cggtgaaggc gtcgccctca acgcccgatc ccagcaactg ccacgcggcg
     6781 ccgtcccagc gcgcgacgcc gtgctgcagg ccgccgccca gcggcaagta gcccgccgcg
     6841 atcaactgcc cgtcaaacac ggccagcgcg ttcaccgcgg tatccccatc gccgcccgca
     6901 aatcccaggg cttgccaggc gctgccgtcc caggccgcga cgccgccggt gatggtgtcg
     6961 cccgcggccg tgagctgacc gcccacgacc agcacttcgg aggccgggcc ggcgccgtcg
     7021 gggtcccacg ttacgatcgc gttaacctgc ccggcagttc ccgcgacgcc gtggcccggc
     7081 agccaatcgc attgctgggc cgctgcgctg ccgacggtca gcagcagagc tatacaactc
     7141 agagcgctca ttgcgccagt tatgccgccg aaaaggccca cggaccgccc gaacatttga
     7201 ctggatgccg acatctctcg cctccgaaac taacaaatcg ctggaatccg aattggtgaa
     7261 cgcgcattgg cgatctgccg cgcacgcttt ttgcaagccg ccggaaccat gcgattccgt
     7321 actgcttggc gtggcgagcg caattgaagc gcaaacccat tgaagcgcga tgcttcgctc
     7381 gtggtgtcct ccgcgctgcg acaaggttta acagaccaca acagttatac gcccagtcat
     7441 taacaaaaag ctattgcatt gcagttgact ttgatgcagc tatttcgatg cagatatgat
     7501 gcaaatgtgg aatctcaata tcgcacgtgg gggatatgta ctatcgcaaa gcgggctgtc
     7561 gcgcgctgcc gggtgctgtc gggtgccatg cccaagcgct aagcgcgggc atgcccacgc
     7621 ggcgcgtatt gcgtcgtcgc tacacgcgat gtgaccgttc tattccgcgg cccgtttgtg
     7681 aatcgagccg atttgtcgga ggcatgcccg cgcccgcaac gcgggcttgg gcatggcagc
     7741 cgggaaggca cccgacgcgg ctccggaatc actgggcgcg gccccggggg ccacatgcgt
     7801 cactcccggg gcagccggcg ccgctccagg gccacggcac cacacgacct ggcggccgct
     7861 gggcaaatcc ggccgaacgg atacaatcgc cccttttagg cgctaataac cggcgaagat
     7921 gaagcggagt gattatgagc aacggcggca gttacgaccc gcatgcggtt gagcctgaaa
     7981 tttataagaa atgggaggat ggcgggtact tccacgccgt cccaaacccc aataagcgtc
     8041 cgttcgtcat cgacatcccc ctgccgaacg taaccggggc cctacacctc ggccacgcgc
     8101 tgaacaacac gctccaggac atccttatcc gccatgcccg catgcgcggc ttcgaggcgt
     8161 tgtggatgcc gggcaccgac cacgccggca tcgccacgca ggcggtcgtc gaacgccggc
     8221 tgaaggaaga gcagaacctg acgcgacacg agctgggccg cgagggactc gtcaagcgaa
     8281 tctgggaatg gaaggagaga ttcggcggcc agattctcat gcagcttcgc aaaatgggct
     8341 gctcgctgga ctggcagcgg acacgcttca cgctggacga ggtctgcgcc cgggcggtct
     8401 acgaggtctt cttccgctgg ttcaaggaag gcctgatcta ccgcggcgtg cgcctcgtga
     8461 actgggacgc cttcctccag acggccgtcg ccgacgacga gatcgtgcac gagacggtga
     8521 aggggagtct gtggcattat cgttacccga ttcttgaagg gatcaaggga tcgagggatc
     8581 aagggatcaa gacgctgtcg gatgagaccg ttcgggcggc tttggcgcgg gccgtggcgg
     8641 cggatgcgaa aatggggcgc gattacctca cgatcgctac cacgcgcccc gagacgatgc
     8701 tcggcgatac ggccgtagcg gtgcacccca ccgacgagcg ttaccagcat ctaatcgggc
     8761 gctattgcct gctgccgctg atgaatcgcc ccatcccaat catcgccgac gcgatcctgg
     8821 tgaagaagga attcggcacc ggctgcgtga aggtcacgcc gggacatgac ccgaacgact
     8881 acgccttcgg ccagcggcac cccgaagcag cccccattaa cattctcacc ccggatggga
     8941 agatcaacgc aaacgggcgc acgcaacggg acggcgggac gcccgccgag ccgtccagcg
     9001 ctgccgggct gttctacgat tacgtcggcc tggaccgcta cgaggcccgc aagcgcgtcg
     9061 tcgccgacct cgaagcgctc ggactgctgg aaaaaatcga gccgtacgag acggaagtcg
     9121 gccactccga ccgcagcaag acgccgatcg agcccatgct cagcgagcag tggttcgtga
     9181 agatgggcga tctggccgag cgggcgatgg aggcggtgcg cgacggccgc gtgcgcttct
     9241 tcccagcgcg ttatgcgaag acgtatctgg attggctggg cgagaagcgc gactggtgca
     9301 tcagccggca gctttggtgg gggcatcgga taccggtgtg gaccagggtt ctggattcac
     9361 ggcagcagtt cgaggagttc aaagcggtac tcaagctggt cgctcgatat cgcagttttc
     9421 aagaactcgt tgcaatagaa gcgtacggga tgggaagcca gaaacccata gagctcaacg
     9481 aactgtctga gacaggcatc agtgggttcg acgaaagagt cctattgatg gtcgtgctcc
     9541 gacatcccga tcgatccgaa ttggtcaggc tctttcgcca ctactacacg ctcacgccgg
     9601 cgcaggctca gttcgattgg ccacagtaca ccgacgaggc atggcgccgt gaccccgagc
     9661 agtttgtaga caagctgttc ggcgatctcg aacagttgca gcgagatccg gacgtactgg
     9721 atacttggtt cagttccgcc ctttggccat tcagcacgct gggatggccg catgatgtaa
     9781 gggatcaagc gagcaaggga tcaagcgatc aagacacggc catcggcgcc caacctctcg
     9841 atccctcgat ccctagatcc cttgatccct ctcccgagga cttcgactac ttctatccca
     9901 cgagcacgct cgtcaccggc cgcggcatca ttacgctctg ggtcgcgcgc atggtcatga
     9961 ccgggctgca tttccccggg cgtgtgccgt tccagcacgt tcacatccac cccatcatcc
    10021 tcgacggcca ggggcggatc atgagcaaat cgctgggcaa cggcgtggac ccgctggaca
    10081 tcatcaccct gtacggcacg gacgccctgc gcttcaccat ggcccagatg gacaccgaga
    10141 cgcaggacgt gcgcatgccg gtcaagccgg tgaaaatgcc tgatgggcgc accgtcaaca
    10201 gcagcgagaa attcgagctg ggccgcaact tctgcaacaa aatctggcag gcggcgacgg
    10261 ggtttgtgtt gcagaacctc aatggcgcgc agccgcggcc gcttacctct gccgacttgg
    10321 cgcttgagga ccgctggatt ctctcgcgcc tggcggccac gatcgccgag gtcgaccggc
    10381 gcctggagca ctaccaggtc agcgaggtgg ccaacctgct gtacaacttc ttctggagcg
    10441 agttctgcga ctggtatctc gagctggtca agccgcgcct gttcgggcgc tcggcggccg
    10501 gcgagatggt gcagcatacg gacgcctcgt cgcaagtcgc gcggcaggtg ctggcgtggg
    10561 tgctcgatca gagcctgcgg ctgttgcacc cgattatgcc gttcgtgacg gaggccctgt
    10621 gggccaagct gaacgccgcc gccccgcagc gcggcatcac gcgcctgatc gaaggcgagc
    10681 cgatgctgat ccgcgcggct tggccggatg ccgccgcgtg gccgcgcgag ccggagattg
    10741 aggacgactt ggcggcgctg cagaacgtga tccgcgctct gcgtgacagc ctggcctgga
    10801 tcaacactac gcgcgccgcg gcgaagacgc cggccatcgg caagctgccc aaagcgctga
    10861 ttcgcgccaa ggatcaactg gttgcgggct tgcaggaaca gaccgcggtg atttgccgtc
    10921 tcgggcgatg cgaagccatc gagatcggcc ccgacctgac caagccgccg gagtcggcga
    10981 cgaaggtctt ccccgggctg gagatttacg tgccgatcgc gggcctggct gacttggatt
    11041 ccgagcgcaa gcgcctggcg aaggagcgcg acgaactgac cggccacctc cagcggcttg
    11101 aagccaagct cgcaaacggg gagtttgtca gcaaggctcc gcccgccgtc gtggaaaagg
    11161 aacgcgctcg gctggtcgaa ctgaaggaaa agctggccgc aatcgggcac catttggccg
    11221 agttgggcga gtaagggcca ccgacgggtg ccatgcccaa gctgtaagcg cgggcatgtg
    11281 atggcctgtt ggcctccgga ccgtgcccgc tgctgacggc gcacatggtt atttgacatc
    11341 gctggatggt tggtacaggc atgcccgcgc ttgcagcttg ggcatggcac ccggcacccg
    11401 gcggttcaac gcttacgacc ggttagtttg aatcgataca gccgtgccag gtggcgcaga
    11461 tagcgaagtt gctccgctac cgacagcttg gtctttcccg cggcgcgatc ggagaatgtg
    11521 atcgggattt cgactacgtt cttccacccg tgccgcacga tcagctccag tgcgattttg
    11581 tagccgatcg ggtccaaacc ctcgaagcgc aggtccttgc ggcgtaccgc gaagaagccg
    11641 gacatcatgt cgcgtacggg cgtcagcggc cgcgccagca gccgcgcgac gaggctgttc
    11701 agccgccgat gcagcggcca gtgcagatcg acacgcccgc ccggcacgaa gcggctgcca
    11761 atagccatgt tgacgcgtgg gtcgcgcagg gcatcgagca gggccggtat cgactccggc
    11821 ggatgcgaca ggtcggcgtc cagcgagacg agcaaatcgc cgcgtgcttc gcgcaagccg
    11881 cgaatgaccg ccgtcgccaa cccgcgctca ccgcgtcgnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnct gattcccact tcgtatacgc gccagccacc gcgggcgatt
    12061 ttggccgtca cttccggctc aaatccgaag cggttgcagc gcagggtcat gttcttgatc
    12121 accggtgcgc gaaagacctt gtagcaggtc tccatgtcgg tcaggttcag gttggtgaag
    12181 acgttgctga gcaaggtcag gaagcggttg ccgaccagat gccagaagta caggacgcgg
    12241 tgctcctcgc cgccggcgaa gcgcgagccg tacaccacgt ctgccttgtc cttcagaatc
    12301 ggcgccagca gcttggggta atcgttcggg tcgtactcga ggtccgcgtc ctggatcagg
    12361 atgatgtcgc ccgtcgcttc cttgaatccg gcgcgcaggg ccgccccctt gccctggttg
    12421 accggctgca gcaccacgcg aaaacgcgcg ttcgggaatt tctgctccag ttgcgggtac
    12481 agatcgcgcg tgccgtcggt tgagcagtcg tccactatca ccaactcacg ctccaggtcg
    12541 atcggcgatt ccagcacgcg gcggatgatc tcgaacaacg tcttcttctc gttgtagacg
    12601 gggatcacaa ctgagagcag cattcgggca ctctactcgt cctatctaac tggtcgctcc
    12661 tcttacgccg ctgccaagcg gcgtggagca tccggccggt tacgtcggcc acggggggcc
    12721 ggcgctacgg ttcgggcacg gctcatcaga ccggcaccgc cagcagcggg tacagggcag
    12781 ccgccggcga cggcatcgcc tcgcgaatct gatcgcgcag cgtcgtcagc ggcaagcgcc
    12841 atagccgccg cgccaacagc ggcgcctgcc ccagccccgg ctcgggcgcg gcgaaggcgt
    12901 ccagcagcag acgttccggc caggtgcggt cgaactgcgg gcggcggaat tcgtcccagg
    12961 tcgccagcag caccggcagc aaagccgctt cccgctggcc aacccagcta agtccggcaa
    13021 aaaccagcaa agcggccgat tcaagccgcg gttctgcgtg atacgccacc atcaggtgcc
    13081 gcacacccag cgcggcgcga ccctggtcca ggcagcagca gccgagcagg taacgggtga
    13141 ccgggcagcg cggccgggca gccgcctcag ccggcaggcg ttcagccagt tgccggagtg
    13201 tgagctgcgc gtagcgctga acagtctgta tcagttggtg gcgtttcatg cctcgcgacc
    13261 agcaccgcac gctaattgtt cgcgtgcccg gcgtcaaccg gcgcgggcgg aagcaccgaa
    13321 cctgctcagg cggctacccc ggccagcgcc ggcggagcga gggttaaaaa acgcggctca
    13381 aaacgcggca tagttcgaac agaatcgcgt cgctgaccgc tggtctgttg ggcggaattg
    13441 acaggatcgc tggagcggcc gcgatgccgc cgaatgggcc atccaccgtg ccgctgttgc
    13501 catcgaagcg tgtcacggtc ccgccgccct gaccctggct ggtgctcagc acgtgccgcc
    13561 agccttccat caggtcgcca ccgtaatcgt tcgtccaaag ccactcctcg ccgacgctgg
    13621 cgccggccgt gcgatcggac cacgccccga ggcccttggc gtccggccct atgattgccg
    13681 catcgccgct gagaatatcc gcccccggta gattcatgcc cagcgagacc agcagcgagg
    13741 atgcggcatc gaacccggcc ggtacgccgg tgctggtgtt tttcagcagg atggacagat
    13801 gtttgccatc aatgtcgaac agggcctgcg ccgccagccc gttcaaaggc gacgggttcg
    13861 cccctccggc atcctgtgtg tagtacacga tcacgtcgcc tggcgcgagc ggcaggccca
    13921 aacagctcaa aaacaatagt gctgaacaaa acgaacgcat ctaacgcctc ctcaagctaa
    13981 ccttcactct gcgcgcatac ggccgcacat tccgctccgc ggcggaatgc gcgccgattg
    14041 cgcctcgcac ggttttcaag ccgtgccggt tcaacggtcc gggttgagtc ggaaccctcc
    14101 ctggtcgccc caaacgtacg ggggttgatc ttagctaatc accggggcgg attcaataca
    14161 aattgagcgg taacgcggtt tttagcttga acgcttctca gcggcgcgct ctacaaggtc
    14221 cgcaaacaaa cacagatgtt cgcgccggtc aatcaggtcc tcaggatgcc actgcacggc
    14281 cagcaagtac tggttctcac agtcctcggc gccctccagc acaccgtccg gcgcaaacgc
    14341 cacggggcgc aacccgcggc cgggataatc ggggtctacg gcctgatgat gcctgctatt
    14401 cacttccagc cgcgtggtgc caataagctg cgccaggcgt gagccgggcg ccagcgtgac
    14461 ttcatgaaag gcctgtccgt gcggcagatg gtgcgcgatg gcgggcgtgc gcggcacatc
    14521 gtcgaggtgc tgaatcagcc gtccgccgcg ggccacgtgc gccacctggt aacccaggca
    14581 gatcgccaga atcggcagac gcagctcgtc ggcgcgccga aaaagcgcca actcaaaacg
    14641 gtcgcgccgt tcgtgcatca gctcggcctt gggatgccgc gtctgcccgt aacgctgcgg
    14701 atccaaatcc aggccgccgc tgaagatcac gccgtcaagt ccgctaagca agtcgtccag
    14761 caaggacggc tcaggttctg ccggcagcgc gatcggccgc ggcagcccac cggcggcgaa
    14821 gaccgcgtcc gagtaagctg cattgagccg cgtttgcagg cgcgggttag gcgggtcagt
    14881 ctgctgaaag ctgcacgtta tgccgatcgc cggtcgtcgc atttagagtt ccagccaagc
    14941 ttgtcggccg cggcggggga gagcccaatt gtctttgccg ccattgcgga ctcgctttcg
    15001 gctgttatat tagcacacaa gcgaggcgcg gtctccggat tctggcccgg gacacaaact
    15061 ttcacagcgt actgcgttgg tgggtcgtgc aggctgtctc ctctaaatgg catggcgttg
    15121 ggtcggcttt gacggccgcc ctggtactcg gggcgcttct cctcattacg cccgtggcac
    15181 gagcgcaaga agccgtcgag gggatggtaa tcaagcgcgt cgaggtttgc ggactggaga
    15241 ccatcagcga agcctacgtg cggcgcgtca tcaagacgcg cgaaggccag cccttccgcc
    15301 gcgcccaagt cgaagaggac gtgcgcgagc tgctgcgcac gcggcgcttc ctcaacgcct
    15361 tcgcggccac cgcggtcgaa gaaaaccagg ccgtcgtagt tttcaccgtc caggaaaagc
    15421 ccgaactcgt ctcggtcgaa ctggaaggca acaaggcctt tcccgacgac cagcttttcg
    15481 ccctcacgcc ggccgcgggc gacgtcatcg atatgtatga agtgcggcgc ggccaggacg
    15541 aaatcctccg caagtaccgc gaagccggct actactacgt cgaagtgacc ttcgatgagc
    15601 gcgccctgca atatgaaggg cggctgatct accgcatcaa tgaaggcccg aaggtccgcg
    15661 tgcggcaaat tctgttcgaa ggcaaccgct cgtttcccga ctggcggctg cgttccaagg
    15721 ttcagaccag aaccgcgctc tggattttcc gcgtgggggc cttcgacgcg gaaaccgccg
    15781 agcgcgacgc gatcgaaatc cagcgctttt atcgcgacga gggcttcctc gatgcccgcg
    15841 ctggttatcg gctcgaattc gacgaggtca cgcgcgccga tctgacggtc gtgttcgtga
    15901 tcgaggaagg gcagcgctac tgtatccagg acatcgtcgt cgaaggcaac acggtctttt
    15961 ccgccgagca catccgctcg tgcatgcagc ttgagcccgg ttacctcctg cgcaccgagg
    16021 ccgtcgagga agaccgcaag cgcgtgcagg acttgtatgg cgaggtcggc tacgttgatg
    16081 cccgcgtggc gatcgaccac gaattcctgg aagagcccgg cctggtcatc ctgcgcgtct
    16141 caatcatcga gaacaaacag tactatttcg gccgcatcat catccgcggc aatcagagga
    16201 ccaaggacga ggtcgtccgc cgcgagctgc agttctaccc cggcgagctg tggaacaccg
    16261 tcgaggcgcg ggccgccgag cagcgtttgc gcgaaaccgg cctgttccag cccgaccaag
    16321 tccgcattac ccccctgccg cccgaggaca gcacgcgccc ggccctggtt gaagtgcagg
    16381 aagccgagac ggtcaccttc ctggtcggca tcggcgtgag caccgatgcc ggcgccatcg
    16441 gttcgctgac ggtcgagaat cgcaacttcg atctgttcga ctggccgcgc acctggggcc
    16501 agttcttccg cggccaggcc ttccgcgggg acgggcagcg gctcaagttc tccgccgagc
    16561 cgggcaccga agtcagccgc ttccgcatcg actttactga gccgtacctg ctcgatcgcc
    16621 ccgtccgtct ggacacctcg ctctatctgt tcgagcgcgg gcgcgacgga tatgacgaag
    16681 agcgggccgg cttcatgttt gcgctaagca aacgtttccc gcgcggcttg ctggccggct
    16741 gggccgtgga aggcgcgttc cgggttgagg gcgtcggggt cgacgacgtc gacgcactgg
    16801 cctcgcggca aatcaaggag gtccggggcg ataacctgct cacgacggtc aagggcacca
    16861 ttgtccgcga tacgaccgac agccgcgtca taccgagcaa gggctaccgt ttcacgctga
    16921 gctgggagca ggcaggcgcc ttgggcggcg acttcgactt cgggcggccg accgcgggca
    16981 tcgcttggta taagacgctg cgcaccgaca tttttgaccg caagagcgtc ctggcgctgc
    17041 acgccgacac gggcatgatc gtaggcgacg cgccggtgtt cgagcgcttc tacgcgggcg
    17101 ggttcggctc gctgcgcggc ttcagctatc gcggcatctc gccacgggcc ggaatcatga
    17161 agaacaaggt cggcggcgac ttcatcttcc taaccggcgc ggagtattcg ttcccgctgt
    17221 acggcaagaa cttccgcggc gtgacgttcc tggatatggg cacggtcgag gagaatgtga
    17281 cgatcactac ctggcgggcg gcggtgggcg tcggcttgcg gataaacgtc gacttcttcg
    17341 gcccggtgcc gatcgtgctc gacttcggct tcccgatcgc cgatgacgat caggacgaca
    17401 cgcaggtgtt caacttcgcg tttggggcct cgttctaggg aacccggttg gggattgaac
    17461 cccgccggct agccctgcgg ctcaaacagg tacaggtcaa tggcctcgat ccggtcctct
    17521 ttttccttca agaagaaccg ctgcatttgt ggctgcccca ggcgacgcgt cagaacgccg
    17581 aggtagcctt cgagctgctc ttccgggaac ggaaccttcc accctcgata agcaaagaac
    17641 cacactcggc cgcccgggtc cggcgccacc gtgtcgggta gcggcgccca actcaacggc
    17701 acaggatgaa agcgcagcac gtcgaacacg aattgggcgc cggtgccgcg ccaatcgccc
    17761 agccaagggg cgtaatcaac ccgctcagtc gcgttgaata ccacccagcg atccccgggc
    17821 cgcgtttgct ccacgacgta gtccaccgcg gcgcggctgc gctgcacggg cccttccttg
    17881 tacggccgga ctgcgtcgcc gatggcgcct cctacagcaa tgagcaagag aacgactgcc
    17941 gcacaggtca ggtacacgcg acacgtcctg ccatgcggcc acttgaacat gattctggcc
    18001 agtgccgcca ggtaattaag aaccctcgaa aggcccagcc cggccagcag gcaaaacgcc
    18061 ggggccatgt aaagcgaggt ccgggccgac ccgccgtacg ggtacttctg cgcggcggcc
    18121 gcaagcatgg tgaacacgag cggcccaagc agcaaccaca gcaggtcgcg ccgcgtgccc
    18181 cagaggcgca ccgcaccgat aacgacgagc agaaggctga acgcgcttgc cggtgctcgc
    18241 ccccccaacg gatacgccag catgtagtcg gtgtgtatga gcaccagcca caggggcagc
    18301 ttccagattt gtgacagagc cagcggcgga aaggcccgct cccacatgtt tatctcggtc
    18361 aaccccgacg cggcctgggc gtgcggccgg ccatatagaa agtacatcag gacaaagctg
    18421 ccgccgaccg cgatcgcgta gcagagtgtt tggagcagca gggtgcgccg tgtggggccg
    18481 ccgctgcacg gctcgtcgcg gctcgcccgc tttcgccggt ccgcagcgac taatcgccag
    18541 gccagcagcg tgccgatcgc ggcgagtacg aagatcaccg ggtaagaaca ccagaccgcc
    18601 gccgctccgc ccacgatcag cgccgcccat gacccggcgc cgccccggtt ctcgcacacg
    18661 cgccatgcca gcatcagcag tcccagcgcc agcagcatgt cggtcgcgta gggcttcacc
    18721 tcggcggcgt ggcgcacgag gtagaccgag gcggcgaaga tgccgatggc cagcagcgcc
    18781 ggacggcgcg gcagtacgct ctgcgcgaag cgccaaaaca gcgggaggct ggcaaggccg
    18841 gcggcgaacg gcaggaggcg caaagcccac tcagacaacc ccagcatccg gctgatcgcc
    18901 agctccgccc acatgaaaac cagcggcacg atctgaccgt acgtcaacgg ctcaatcatc
    18961 tcggcgtagc cgcgcttgac gaagtcgacc gccacgaacg cttcgtcgcc ccagattggg
    19021 aagccgagcg cgtagcgaac aatccgccac agcacgccgg ccgccagcag cgctacgagc
    19081 agaatgctcc acgtgcgagc gtgtcggcga gcggcggtcc gtgcgacgct gcttgaagcc
    19141 tcagcttcga gcagcgcggc agccggcgcc gcggttgggt gcacccctgt cgtggtctgc
    19201 tggtccatct cctctccgaa ggctgcggtt ccggcgtcgc cggtgataag tcgaccgcta
    19261 gctcgcgccg cgccggcgcc gattgacttc tcgacgagcc gcggttagtc tacccggact
    19321 caggattttt ggcccccatc gtctagtggc ctaggatatc aggttctcat cctggagacc
    19381 ggggttcgag tccccgtggg ggcgtgagtg ccaagcacca gccacgtccg gctcggaaga
    19441 gccggacgtg gcgtttttta ttccccgcgg acgaatcccg agcacaactc gttaaacagg
    19501 ccatggcagg acggccgtaa ccgcgattct ccatatccgg acgggcggta accccgattc
    19561 ttacatatcc atccggtcgg cgggcttgac gggcgtgact atatagttat atgtgaacgg
    19621 agcgtaacac cggcgcgtcg agcccgcccg gtgacggccg tccggaaaga tgctcgatta
    19681 cacccccgtc agggaccccc tcaagcgctc cgcggctgag ttgcgttccg agctgcgctc
    19741 cgagtggatc agtccccaaa cagccgtcga agcgccggta ctctcccgcg cggccgccag
    19801 ccacccccgc cgagagcggc tcagtttttc caacccgatc gcgcgcggcg ccgacccctg
    19861 ggtcattcaa catgccggcc actattacta ctgcgagccg gttaccgata cctccattgc
    19921 gatctggaaa accgactgct tcacgcggcg cggcacgcgc caaatcgtct ggaaagcccc
    19981 gcgccagggc tggaacagca acctgatctg ggcgcccgag ctgcaccgtc tcgacggccg
    20041 ctggttcatc tactacgccg ccagcgccgc gccgcgcgag aacgccagcc accgcatcgg
    20101 cgtcctcgag tgcctgacgc aagacccgct cggcgcctac atcgaccgcg gcatgctcta
    20161 caccggcgat gatccgctct gccgctccga caatcgctgg gccatcgacg ccaccgtgct
    20221 cgaactgcac ggacaacgct acctgctctg gtcgggctgg ccggacggcg cggacctgca
    20281 atacctctac atcgccccgc ttaaaaaccc gtggaccacc gccggtccgc gcgtccggct
    20341 gtgcgacaac gcgacgttcg actgggaacg cgtcagcgaa gacccgcgcc aacgcggcct
    20401 gcacgaagcc cctcaggtac tgaagcacaa cgaccgcgtg ttcgtcatct actcgtgcag
    20461 cgcctcctgg caggagacgt acaagctggg tctgctcgaa ctcatcggcg acgatccgct
    20521 taaccccgcg acctggcgca aacatccgca acccgtgttt cgcagttccg gcgacgtctt
    20581 cggcgtggga cacgcaagct ttgtcaaatc acccgacggc ctcgaagatt gggtggtgta
    20641 tcactgcaaa acctcgcgcg ccccgggctg gcggcgagtg gtgtgcgcgc agcgcttcgg
    20701 ctggcggccg gacggcttgc cggatttcgg ccagccggtc ccgtggcggc aaccgctcag
    20761 cctgccgtcc ggttctggcg gcgtcgatgt tctgctcgat ggttctgaag acggcatttc
    20821 ctacccgcta cgtcagccgc gctgtccgcg catggaggcg gccaattcgg cccgcgtaga
    20881 cctggcggaa tcgcgctacg cctggcgctt cggccaatgc ccgtgcgagg cgccaatatc
    20941 gtttgcggga gcaggggtta ttcgcgactg ccggttgtgc cgcgcgacct caacgagcgc
    21001 ttgattcgct aaggcaccgg cgaccgacaa gctcactccg gaatttccaa aatgaaacct
    21061 caaccacccg tcgcaggtcg cactacgcgc ctataagtat tgtgccagcg gccggttgca
    21121 gcgtctgggc ggcggccgga tcgtgctttc gataggatgg tgatggcccc ctactacgaa
    21181 gaaaaaatga gcggtcgatc cggagtgttg ggtccctccg cacccggcgg tcttttccag
    21241 agtttcttca tcgggggctt cgagtgctcc tcgcacaagc gacgcagtgg ccgccggctc
    21301 gacatgatcg ccgccaccga ccatgaccgg ctcgctttgt acgactacac ctcgctgaac
    21361 gctctcggca tccgcaccgt ccgagatggc atccgctggc accgcatcga acggaccccc
    21421 taccactaca gctttgccgc cgacctgccg atggtccgcg ccgcgcgaca gaccggcaca
    21481 caggttgtct gggacatctg ccattacggc tggccggatg acctttct
//
LOCUS       DAOSYS010000032        20782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2095_length_20782_cov_5.453997, whole genome shotgun sequence.
ACCESSION   DAOSYS010000032 DAOSYS010000000
VERSION     DAOSYS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20782
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_20782_cov_5.453997"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLU99_03375"
     CDS             <1..310
                     /locus_tag="PLU99_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16129.1"
                     /translation="RAARDPQEHDQTLAKAYLSLADYYAQRGDHDLEREFCYRAARLR
                     PDDPALALRLADAEWLAQHYEPAARHYRQYLLHSPPQDTERQRVLERLATTPAASGAP
                     "
     gene            412..511
                     /locus_tag="PLU99_03380"
     tRNA            412..511
                     /locus_tag="PLU99_03380"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:447..449,aa:Sec,seq:tca)
     gene            complement(655..1275)
                     /locus_tag="PLU99_03385"
     CDS             complement(655..1275)
                     /locus_tag="PLU99_03385"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOO16130.1"
                     /translation="MESSHPQCSPMQPLVTRVAPDFVAQAVLPDNTFAELRLSQFRGK
                     YVVLFFYPLDFTFVCPSEVIAFDKKLPAFRERNCEVIGVSVDSHYSHWAWKNTPREKG
                     GIGQVQFPIVADLTKKIARDYGVLLEDGGVALRGLFLIDREGVVRHAVINDLPLGRNV
                     DEALRMVDALRHYEKHGEVCPANWRQGEEAMKPSADGVAKYLAQHG"
     gene            1498..1944
                     /gene="dut"
                     /locus_tag="PLU99_03390"
     CDS             1498..1944
                     /gene="dut"
                     /locus_tag="PLU99_03390"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOO16131.1"
                     /translation="MNAVLKVRRRPGTEDLPLPQYMTAHSAGLDIYAANDEPVTIAPG
                     DVKLIPTGLYLEIPPGHEGQVRARSGLALRHGLMLPNAPGTIDADYRGELQVILGNCG
                     REPYTVTRGMRFAQLVIKKVEHVEVLEVPELADSQRGAGGFGHTGH"
     gene            2151..3410
                     /locus_tag="PLU99_03395"
     CDS             2151..3410
                     /locus_tag="PLU99_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOO16132.1"
                     /translation="MRRRRRRSRRGRVVGTIVVVVGLAAAGTWFFVGKGYLPLQRAEA
                     KPAAAAPVPIIATEDEDAHWENPRVPTLDELAARDKPLRPRLGGGETSTELAAAKPEP
                     AADPAVAMIVEGEAGPRPGALKPVAPPSGPAEQSPSDVSAAETPPADKPAAADRTTPD
                     AAPAGDQGATGNARIDAALKLLADGKIIEARHALNALLAQPLPRNEQAEVRRRLAKIA
                     DDSIFSRRSLPGDPLADTYTVQSGDRLVNLSRDYKVPAEILMYINGIQDATKMRAEQK
                     LKVLRGPFHVKISKSEFRMDVYLQDLYVRSYRVGLGADQGTPEGVWKVKDRLERPTYY
                     PPASAEIKRIIPPDDPENPLGNYWIGLEGVSGDAVGHTGYGMHGTIEPESIGQAASMG
                     CVRMLNEDVEFLYKLMLPGESTVTVLP"
     assembly_gap    3576..3675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3676..4471
                     /locus_tag="PLU99_03400"
     CDS             <3676..4471
                     /locus_tag="PLU99_03400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO16133.1"
                     /translation="AENPSQPLRLADTICWILYPPCMRLGPVLTRESFLKRFDAWAPA
                     QRVDWATVGRRAGLLLLGLVGLAITIRNMPSRQGEIDFFSAPQQYTTNDLLALIYLVP
                     VQIYFLLWCYNQLANGLAAWVGIPVDDNFDHLPLATSVRDFWRRWHITVGAWLRENIY
                     IPLGGNRSIVWLNYVAVFGYIALWHGPSWSFVAWGLLQAAALMAQRAWDQFWARRNHR
                     GRPAGLPWTIFCWLLTMHFAIITIVIFTDFEHLGTRLFPELLRRLF"
     gene            4622..4882
                     /locus_tag="PLU99_03405"
     CDS             4622..4882
                     /locus_tag="PLU99_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HOO16134.1"
                     /translation="MAEQLVGKVTHYFAKAGVAAIKITDGELHVGDTIHVVGHTSDFK
                     QPVDSMQVNHAPVAVAKPGDEVGIKAAGHAREHDEVFKVLPD"
     gene            5162..5287
                     /locus_tag="PLU99_03410"
     CDS             5162..5287
                     /locus_tag="PLU99_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16135.1"
                     /translation="MKAHRLPLLLGLSGLALSALIPPALASVERVVLCEEFTNTG"
     gene            5456..7087
                     /locus_tag="PLU99_03415"
     CDS             5456..7087
                     /locus_tag="PLU99_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16136.1"
                     /translation="MLQRVGGSSGMSYLAQYSTRHGIPSEYTIQGSAAHLGGQTYRIS
                     ATVCSQASTSRTVRVQMVHVLDYYPDSPTYSRNCLRQASATQDITLLPGACEQVAPWD
                     ITFDATSWARQSDITILIWIQQASGGREVYQAEIMNWPLLTDCNGNSIPDECDVDCSS
                     PGCSTYPGCGGSQDCNANGVPDECEADCNLNGIPDDCDVDPTDPDGDGLVSSDCNENG
                     RPDECEEGGQSDCNSNGVPDLCDIYAETSQDCNQNHIPDDCDIADGTSEDCQGTGIPD
                     ECEMMPPAFPEAYDSCLDAEIACPGTVYAGTTEGATVDGSASCGSSSSTPDVWYYYTA
                     MGNGFASFSLMGSSYDTVLSLHSDCPGTTDNQLICNDDYGGTPQSRIATYFVQTGRTY
                     WIRISGKNGAVGDFVFSMTGPGCLYTKPDCNRNDVLDACELLDCEPTNPACQDCNENG
                     VLDECDIASGYSQDADGDGRPDECAAPCKLGDSNCDGTVNVFDIDPFVIALTDQPAWE
                     AAYSCGFLCANDCNGDGTVNVFDIDPFVQILTGSN"
     gene            complement(7184..8695)
                     /locus_tag="PLU99_03420"
     CDS             complement(7184..8695)
                     /locus_tag="PLU99_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOO16137.1"
                     /translation="MRIILVGAISLLLAGMSAYRAQGGEVTDEVKMRISEAAPRRAPA
                     QAQKPRSLLVFTLCRGFRHDSIPTGAAAMKILGEKTGAFTAVESDDIDMFTPEKLARF
                     DAICLMSCTGELFRPANFEQLPAAEQEAARRRDEALKKSLLDFVSGGKGLIGSHAATD
                     CFYKWPEYGEMMGGYFDGHPWNEPVTVRLDEPNHPLNAAFDGKPFVIADEIYQFGAPY
                     SRDKLRVLLSLEPTKIDPAKPGIKRTDDDFAISWVRQYGKGRVFYCSLGHRDEIFWNP
                     QVLAHYLAGVQFALGDLSADAEPVPHTTGESPLGVITLFNGKDLSGWICKPGSWAAED
                     GVLARRGGGDIWTEQQFGDFILDLEFKIDKGANSGVFFRTADINDCVQTGIELQVLDS
                     YGKAAVDKHDCGAIYDCLAPSKNAVKPAGEWNTLRLVCRGPRLQAVMNGLVIINMNLD
                     DWKEAGRNPDGSPNKFRTAYKDMPRAGHIGFQDHGGGIWYRNIRIKPLPVTPG"
     gene            complement(8857..11517)
                     /locus_tag="PLU99_03425"
     CDS             complement(8857..11517)
                     /locus_tag="PLU99_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO16138.1"
                     /translation="MIKPDSCRLARVTNHAPRQLLRCAIVLTALAACAMAAPSAELER
                     LLRRIDFHGAAKPDVLGKALAELPPSDWATLCDMLVEAGAGDDTKPRMALEGLAWYTG
                     SSGTPAQRAAYIEVLTAALSKDRQPGVKAFLVRQLQLVGDDSAVPTLGKLLLDPEVYD
                     FVAQALVTIGSDAARAALRAAYPEAQGPCRIAIIQALGVLRDSESADRLAEDANGPDR
                     GIQLAALAALATMESPAFREAARKFESQTHYERSQLDALLFRTSAHAAEQGEPEADAA
                     LTAELQSADPHIRSAALSSAAGVKSIDLVLSFLADEDAEVRATATNIAISLQGEGVTA
                     AYLERLPKATETGRVALIGILGKRGDAAALSAVFEALQDESPAVRLTAVEAAAALGRT
                     AAVEPLIKLLSDDGDLSAAAQRALTNIRDEQAAAQIAGALSGSPPRVQARLLQILADS
                     HAVEQAGAICGCLEKDDEGVRLAALRAVEALAPSSALDPLVRLLPKLENSEQREACES
                     ALTAVCRRAADRVAVSGKLLAALPAARAEDYCSLLRVLGKLGGAEAFQTVRQALSDSR
                     SEVVEAAFRALSTWPDPAAAGNILEVATGSGELRHHVLALRAYVRLVGADQELSAADK
                     LAAYEKCMAHTRRVDERRLLLSKIGELPEPRTISVLQRFLLDDQLRNEAGAALLGVAE
                     RQLPAGWQRARQAIWLALDAVDDDALRERGKALLARAEEYEGFITDWQVAGPFDQDGK
                     GCLELLEVAFPPEQPDIGGVTWQRQTMPTDRPEFWYVDLNACADMAGSNRAAYLRTVL
                     DSPRAQDVQLELGSDDALKVWLNGELIHTFAGVRGCERGQDKVPAQLKKGRNELLLKV
                     VNGGGGWAACARVRRPDGSHVEGLKIDLGQ"
     gene            complement(11529..12824)
                     /locus_tag="PLU99_03430"
     CDS             complement(11529..12824)
                     /locus_tag="PLU99_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO16139.1"
                     /translation="MPRPIKLTRRKLLGTAVKAGAVFYIVPRHVLGGRGYTPPSEVLT
                     RAVIGAGGMGMAGHVTENVEGQPPQTLAVCDVDKNHLAAAMAKAGRSCEAYSDWRRVL
                     DRTDIDTIHIATPPHWHAVMGVAAAQAGFDILTEKPFSHTVAEGRAYVETVKRYGRIL
                     QINTHFRFEQYYDYGATKLLRKLVASGLLGKPLLVRVGAGQGFNWKLQEWSGRTNLTP
                     EPVPAELDYDMWLGPAPRKPYHPHRVHQSFRGYWDYDGGGLTDMGQHYLDPVQYFLGK
                     DHTGPVEVSAVAPWPPHPDAVGVWESVTLKYADGDTIYMNSGEWGSPEPPGLPLIEGP
                     KGKVYRNYRTDPPGLFEQLERVPDPPPLIDFETAVKTRQKAGGNEEVAHRSCTLLNLA
                     SIAIRLGRPLKWDPDQERFIGDEQANRLIEVPMRAPWHL"
     gene            complement(13062..13997)
                     /locus_tag="PLU99_03435"
     CDS             complement(13062..13997)
                     /locus_tag="PLU99_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOO16140.1"
                     /translation="MSFAMGADPQTGTSPLLADPVHVFEHRAMNCGWGIFIVGDDERY
                     AGQAAQAAFGEVDRVERELSRYIPTSDIARINALAPGQSVRIGPDTFACLEIAAQVGA
                     ATHGAFDVTIGSLLGPGPHRVPARRVPRIGVDEESRTVTALSESVLLDLGGIGKGYGV
                     DQAVAVLRDWKITAALVHAGQSTTFALGAPGGEPGWTVAVRDPRQPESSLARFYLRDQ
                     AFSGSGMLLHGEHIIDPQTGRPPNTGALGAWAIAATAAKADAFSTAFMILSPSEVANY
                     CRRHSQITGMLCLDSPPGARLETFGREPQWVMQPV"
     gene            complement(13994..15625)
                     /locus_tag="PLU99_03440"
     CDS             complement(13994..15625)
                     /locus_tag="PLU99_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOO16141.1"
                     /translation="MKPEHLYRLIFALIVAGGLALRLPRLELRPMHGDEAVHAVKFET
                     LWQTGDYVYDPHEYHGPVLYYATFPFVWLSGAQSLAELDEQTLRVGPALFGAGIIALL
                     LLLLDGLGWRVVLATAALTAVSPIMTFYSRYYIQEMLLAFFALLVICAGWRYWQTGNP
                     GWALLGGVGVGLMHATKETSIISLGCMAAALGVVAVWQRRSGTKERAGEEPEKGIEGS
                     RDRGIKKNVVVWGDLVVAAGVAILVSVLFYSAFFRHWRGPLDSVLTYATYFERAGGHG
                     LHDHPWYYYLQMLGFTHLARGPRWSEGLILGLALAGIGVAVTGAGLSDQPRRLGLVRF
                     IAVYAVLMTVVYSVIPYKTPWCAVQFVQPLIMLAGLGAVGIISWVKPWPAKAVAAVIL
                     LAGAAHLGWQAQRANFRFFVDYRNPYVYAHPLGDVKYLSAYLEKLADVHPDGYRMLVK
                     VIAEDAWPLPWYLRGFERVGYWEAPPAELDAPVVIVAGDLAASLPAGFENDYKISFYG
                     LRPDIQLVTYVERELWQEFARRQEHLAPGPVRERE"
     gene            complement(15622..16368)
                     /locus_tag="PLU99_03445"
     CDS             complement(15622..16368)
                     /locus_tag="PLU99_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16142.1"
                     /translation="MMKLSKLSRLPAALSATLIVAAVLSLADDGAPGAAGAQGEVAKE
                     SKAAGDLVPLNIKLPKPAFRGTPKHVPPGTNLEEPRKGPRPPFMAAKGAVNVAAGKPV
                     TSSDSEPIIGSIKLVTDGDNSADEGSYVELGPGLQWVQIDLKEKAEIWAILVWHYHAN
                     ARVYHDVVVQIADDPDFITNVRTLFNNDHDNSSGLGLGRDKEYWETYEGKLIEGKAAV
                     ARYVRLYSKGSTADDQNHYIEVEVYARPAK"
     gene            complement(16283..18790)
                     /locus_tag="PLU99_03450"
     CDS             complement(16283..18790)
                     /locus_tag="PLU99_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16143.1"
                     /translation="MPLVNALTVRMRLVNGITIGLVAILAPLAGVPRAFGQEAGRENE
                     LKTGSRAPYVHRLTLYDENGVAIDPRDESAGPYSPRMTCGKCHPCGEIAGGWHFNAKQ
                     GGAAAGRPGEAWLLVDLPSGTQVPISGRGWAGTFRPADEGLSSWKFIKRFGRHTPGGG
                     YGMPDADELRSAPEKARWAVSGPLEIDCMFCHSADLQHDPAEAAVQIEAENFKWAATA
                     ALGLAAIRGEARKAPDDWDPALPPSPDHPERDGPRMVWNKSRFDVDDRVLFNIVRRPP
                     NERCYYCHSVREVGPKARAEERSCRDVHLTAGLLCVDCHRNGMDHMMARGYDGEAGER
                     ADAGLAAFSCAGCHLGVEGASQAELAQGGRYAAPRPEHWGLPPVHLEKLTCTACHSGP
                     WPEMDAHRFQTSLAHGLGLATRERDADTPPNIVGPVFARQPDGKLAPQRLIWPAYWGR
                     LTGEGVEAYALDSVLKTLKKVDPKRAKASAEKLAALTEEQIGAVLEQLGKTDAGHTPV
                     YVRDGLLYRRGPSGELQKRAFETPAEEGSHGLSPQQANAIQLATAPYRWSLAHDVRPA
                     TQALGIGGCTDCHAQGAPIYYGSIAAAGVPSGPEAVQTMYRFRGEDGAKVQAWAAGFV
                     FRPAFKWFGFACAAVVALLLLRVVLNVGSAGGGDGRLGVRAARMEKLVLGLGLVGLVV
                     QLVTGLGTKWTLGRTEGWALLIHMLGAPLLILGVTGAAYFGSARRRCWAGSANSALQK
                     TQAVVYWLSVTCGFVTMTAMLAAMTPLFGTAGQKSLSLLHERAGIGLVIALVMYLVVS
                     VLLGRRRGIDDEIEQAVAAAGRLERDADSCSGSITGR"
     gene            complement(18889..20262)
                     /locus_tag="PLU99_03455"
     CDS             complement(18889..20262)
                     /locus_tag="PLU99_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO16144.1"
                     /translation="MMGEAGNSESGITRREFVQTAAAAGLAMTMPAWAQAPKEAAGGA
                     AASEAAGDELAVGIIGSGSQGRNLLLNCLKIPGVRFVAVCDIWPYSRRYAINTLKKYD
                     QVVNGYEDYREMLDKEKHLDAVIVATPDWMHAEHTIACLKAGKHVYCEKEMANTTQSA
                     QEMVRAMRETGKLLQIGHQRRSNPRYWHALKMIEKDKILGRVTHCYGQWNRPRLYEIG
                     CPKGEELDAEVLKRFGYDTMERLCNWRWYRKYSGGPMADLGSHQIDIFNWVLKTVPRS
                     VLASGGLDYYTEQEGRDWYDNVMAIYEYQTAAGTVRAFYEVLNTTSNGGYYETFMGDE
                     GTLVISEDPKKGHIFREQAAKRREWENEADKVELMDREAIELKIGETLTASGEKDPEG
                     QRLLEESQKPPHQLHLENFFTAVRTGSPLSCPADLAYEVCVSVLKANEAVESGKRCEF
                     KPEEFKV"
     gene            20506..>20782
                     /locus_tag="PLU99_03460"
     CDS             20506..>20782
                     /locus_tag="PLU99_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004795688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16145.1"
                     /translation="MRRFNAFAVLTIAAVFAVAAYAASRQNTGQPAQAGQPAKPSQSA
                     QNAQPGRNAQPVQPAEPAQPAKPLQPAQPAKPAQPANSPAPAASAPAQ"
BASE COUNT         3820 a         7214 c         5945 g         3703 t
ORIGIN      
        1 gcgcgccgca cgcgacccgc aagagcatga ccagacgctg gcgaaggcct acctcagcct
       61 ggccgactac tacgcccagc gcggcgatca cgacctggaa cgcgaattct gctaccgcgc
      121 cgcgcgcctt cgtccggacg atcccgccct ggccctgcgc ttggcggacg ccgaatggct
      181 ggcccagcac tacgaacccg cggcccgcca ctaccgccaa tatctcctgc acagcccgcc
      241 gcaagatacc gagcgccaac gcgtcctgga gcgcctggcc accacgcccg ccgcctccgg
      301 cgctccctga accacgaagg gtaatcagag cgctgcccca ctttgtcgct catatgctca
      361 taactcgcac tggcccaccg ccccgccgcc gactaacatg cttcgcaatc agggagcgtc
      421 cgtgcacctg gtgggcaccg cggccttcaa agccgttgaa gggctgtacc cgcagtcctt
      481 ggtgggttcg attcccatcc gctcccgtcc aaattgccgt tggcccccac gatacaatga
      541 aaggcgaggc tcctcagccg cggcttgcca agagcctcgc ctgaacaccc gccgttctaa
      601 gcacgcgcag aacggcagga cattccttgt cttctcagag catgctaagt cacatcatcc
      661 gtgctgggcc aggtatttcg ccacgccgtc cgcgctcggc ttcatcgcct cttcgccctg
      721 ccgccaatta gccgggcaca cttcgccgtg cttttcatag tggcgcagcg cgtccaccat
      781 tcgcagcgcc tcgtccacat tgcggcccag cggcaaatcg ttgattaccg cgtgccggac
      841 gaccccctcg cggtcgatca ggaacaaccc acgcaacgcg acgccgccgt cttccaggag
      901 aacgccgtag tcgcgcgcga tcttcttcgt caggtcagcc acgatcggga actgcacctg
      961 cccgatgccg cctttctcgc gcggcgtgtt cttccaggcc cagtgcgaat agtgcgaatc
     1021 caccgaaacg ccgatcacct cgcaatttcg ctcgcgaaac gccggcagct tcttgtcaaa
     1081 cgcgatcacc tccgatgggc agacgaacgt gaagtccagc ggataaaaga acaacaccac
     1141 gtacttgccg cgaaattgcg acagtcgcag ctcggcaaac gtgttgtccg gcagcacggc
     1201 ctgagcaaca aagtccggag ccacgcgggt gaccagcggc tgcattggcg aacattgcgg
     1261 atgcgaagat tccatatctc tcccttcttt ttacctaacc cgagcttctt acagcccgaa
     1321 cacctgttgt taaacgatag tcgcgcgatt cccgcgttca agtggcgccc ctcgtgtggc
     1381 atgcccgccc aaggttgcca cgacaaacca gccatgccgc caacacttgc gccgccgctt
     1441 ttcgctatac tgccgggcaa atttgccaac cgcattgcgg gctgaatgga gcaacggatg
     1501 aacgccgtcc tgaaagtgcg tcgccgaccc ggaaccgagg acttgccgct gccacagtac
     1561 atgacggctc actcggccgg cctcgacatt tacgccgcca acgacgaacc cgtcaccatt
     1621 gcaccgggcg acgtcaaact gattcccacc ggcctttacc tggaaatccc gcccgggcac
     1681 gaaggccagg tccgggcccg cagcggattg gccttgcggc acggcctgat gctcccgaac
     1741 gcgccgggca cgatcgacgc tgactaccgg ggcgagctgc aagtgattct cggcaactgc
     1801 gggcgtgaac cctacactgt tactcgtggc atgcgcttcg cccaactcgt catcaaaaaa
     1861 gtcgaacacg tcgaggtcct ggaagtgccc gagttggccg attctcagcg cggcgcaggc
     1921 ggcttcggcc acaccggcca ctgaggaatg ccaactgcaa cggctcagtc aatagtttcg
     1981 acatcgaggc gttcgcgctg gcgccggcag gcccacgcaa tggcaagccg ggtacccgcg
     2041 tgattgcctg tatgggggcg cgactgctgg cctgttgccc aaaaaccggt tatcctgcac
     2101 acgtcacgga aggcaagcag acctttttgg atggatccag gaggttccag gtgcgacgca
     2161 gaagacggcg gagcagacgc gggcgggtcg ttggtactat tgttgtcgta gtgggattgg
     2221 cggccgctgg gacgtggttc ttcgtgggca agggatacct gccacttcag cgcgccgagg
     2281 ccaagccggc cgcggctgca ccagtgccga tcatcgcgac tgaggacgaa gacgcgcact
     2341 gggagaaccc acgcgtgccg acgctcgacg aactggccgc ccgcgacaag ccgctcaggc
     2401 cacgcctcgg tgggggcgag acgtccaccg aattggcggc agctaagcca gagcccgccg
     2461 ctgatcccgc cgtagcaatg attgtcgaag gcgaggccgg tccgcgcccc ggtgctctca
     2521 aaccggtcgc gccgccaagc ggcccagccg aacaatcgcc cagcgatgtc agcgccgccg
     2581 aaacgccgcc tgctgacaag cccgcggctg ccgacaggac tactccggat gccgcgcccg
     2641 ccggcgacca gggcgctacc ggcaatgcca ggattgatgc ggcgctcaag ctgttggccg
     2701 atgggaagat catcgaagcc cgccatgcgt tgaacgcgtt gctggctcag ccacttccgc
     2761 gaaacgagca agccgaagtt cgccgccggc tcgcgaaaat tgccgatgat tcgatcttct
     2821 cgcgccgcag cctgcccggc gacccgttgg ccgacacgta cacggtgcaa tcgggtgacc
     2881 gcctggtgaa cctcagccgc gactacaaag tccccgccga aatcctcatg tacatcaacg
     2941 gcattcagga cgcgacgaaa atgcgcgccg agcagaagct caaggttctg cgcggcccct
     3001 tccacgtgaa aatctcgaag tcagagttcc gcatggacgt ctatctgcag gatctctacg
     3061 tccgcagcta ccgcgtcggc ctcggcgccg accagggcac gcccgaaggc gtttggaaag
     3121 tgaaagaccg gctcgaacgc ccgacatact atccgcccgc ttcggccgaa ataaaacgca
     3181 tcatcccgcc ggacgatccc gagaacccgc tcggcaacta ctggatcggc ctcgagggcg
     3241 tatcgggcga cgccgtcggc cataccggct acggcatgca cggcacgatc gagcccgaat
     3301 ccatcggcca ggcggcctcc atgggttgcg tgcgaatgct gaacgaagac gtggagttcc
     3361 tgtataagct catgctgccc ggcgaatcca ccgtgactgt tctgccttga acgagttcct
     3421 ccaggcgcca cgactgttta atccgctact ggcgccaacg agccagccgc tcaccccgtt
     3481 cgaaatcgcc gctggcccgt acggcttgct gctgttcgtc tggttggtgc cgctggtgcg
     3541 aatcgttggc cgtcgccggc cgcgcacggc catcgnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnggcgg aaaatccaag tcaaccgctt cgtttggcgg acaccatctg
     3721 ttggattctg tatccgcctt gtatgcgcct cggtcctgtg ctgacgcgcg aaagcttcct
     3781 gaagcgcttt gacgcctggg ctccggcaca acgagtggat tgggcgaccg tcggacgccg
     3841 cgccggcctg ctgctgctcg gcctggtcgg cttggcgatt accatcagga acatgcccag
     3901 tcgccagggc gaaatcgact tcttcagcgc tccgcagcaa tacaccacta atgacctgct
     3961 ggcactgatt tatctcgtgc ccgttcaaat ctacttcctg ctatggtgct acaaccaact
     4021 cgcgaacgga ctggcggcct gggtcggaat tccggtcgat gacaattttg accatttgcc
     4081 gctggctacc tcagtgcgcg acttttggcg gcgctggcac atcaccgtcg gcgcctggct
     4141 gcgcgaaaac atctacattc cgcttggcgg caaccgcagt atcgtctggc taaactacgt
     4201 ggccgtcttt ggctatatcg ctctgtggca cggcccaagc tggtcctttg tggcgtgggg
     4261 actgctccag gccgccgcac tgatggcaca acgcgcctgg gatcagttct gggcccggcg
     4321 caatcaccgc ggccgccccg ccggcctgcc ttggacgata ttctgttggc tgctcaccat
     4381 gcacttcgcg attatcacca tcgtgatttt cacggatttt gagcacctcg gtacgcgcct
     4441 gttcccagag ctgcttcgca ggctgtttta gcaaagccaa attcacaatt gcgatttggt
     4501 gacgcgctgc tcatgttctc tgctgggctc gagaattcgc ctggagctat cgccattgtt
     4561 gctagagcgc cctgatcggc tatgatgtac ttggcaaatc gggcgggtaa ggagttcgac
     4621 aatggccgaa cagcttgtcg gcaaagtcac gcattacttc gcgaaagccg gcgtggcggc
     4681 gatcaagata actgacggcg aactgcacgt cggtgatacg atccacgtcg ttggccacac
     4741 ctctgatttc aagcagccgg ttgactcgat gcaggtaaac cacgcaccgg tggcggtggc
     4801 caaacccggc gatgaggtcg ggattaaggc ggccgggcac gcccgcgagc acgatgaggt
     4861 gttcaaagtt ttgcccgatt aaggttcggc tccgccaaca tgccctgcac acacgtgccc
     4921 cgcccgctgt tgctacgggt tatgggacgc gtgcccccat aacgaaactc gcgaaacaaa
     4981 acgttccatt tggatcgcaa ttctgctagg ctacgttcga atctggtcga tgccgcactg
     5041 ccccgtgatc tgctccggca accggtagcg cacgagccga aatgggcggg ggtcaggggt
     5101 agtttacgcc cggcgtcggg cgccgagcgg ctgaggtcgc agttatcttt aggaggcaaa
     5161 gatgaaagca caccgccttc cgttattgct gggcctgagc ggcctggcgc tctcagctct
     5221 gattccgccg gcactggcga gcgtggagcg cgttgtctta tgcgaagaat tcacgaacac
     5281 gggctgaggc tactgctcct acgccggtca ggcgttcggt gccctgctca acagctatcc
     5341 cgataccttt gcgggcaatc aaatccacgt cggcgacgcg tacgccacga gttggggcag
     5401 ctcacgcgcc agctactaca gccttagcgg cactcccacg gcctggttcg acggcgtgct
     5461 ccaacgcgtc ggcggctcgt ccgggatgag ttacctcgcg caatacagca cccgccacgg
     5521 tattcccagc gaatacacta tacagggttc ggcagcgcac ctcggaggcc agacttatcg
     5581 gatttcggcc accgtgtgtt cgcaggccag cacctcgcgc acagtgcgcg tccaaatggt
     5641 tcacgtgctc gattattatc cggattcgcc cacctactcg cgcaattgct tacgccaggc
     5701 ctccgcgacc caggatatca cgctgctgcc cggcgcctgc gagcaagtcg ccccctggga
     5761 catcacgttc gacgccacaa gctgggcgcg ccagagcgac atcacaattc taatctggat
     5821 tcagcaagct agcggcggcc gcgaagtcta ccaggccgaa ataatgaact ggccgctgct
     5881 caccgactgc aacggcaata gcatccccga cgaatgcgac gtcgattgca gctccccggg
     5941 ctgcagcacc taccccggct gcggcggctc gcaagactgc aacgccaacg gcgtgccgga
     6001 cgagtgcgag gcggactgca acctgaatgg catccccgac gattgtgacg tcgatccaac
     6061 cgacccggac ggcgatggcc tcgtcagctc ggactgcaac gaaaacggcc ggcccgacga
     6121 gtgcgaagag ggcggccaga gcgactgcaa cagcaacggg gtgcccgacc tgtgcgacat
     6181 ctacgccgag accagccagg actgcaacca gaatcatata ccggacgact gcgacattgc
     6241 cgacggaacc agcgaggact gccagggaac cggcatcccc gacgagtgtg aaatgatgcc
     6301 gccggccttc ccggaggcct acgattcctg tctcgatgcg gaaatcgcct gcccgggcac
     6361 ggtctacgct ggcactacag agggcgctac cgtagatggc tccgcaagct gcggctcttc
     6421 gagcagcacg ccggacgtgt ggtactacta caccgcgatg ggcaacggct tcgcatcctt
     6481 ctcgctgatg gggtcgtcat atgacactgt gctttcactg cacagcgact gccccggaac
     6541 aactgataac cagctcatct gcaacgatga ctacggcggg acgccccagt cgcgtatcgc
     6601 cacctacttc gttcaaaccg gacgcactta ttggattcgc atttccggaa agaacggcgc
     6661 cgtgggtgac tttgtgttct cgatgaccgg cccgggctgc ctgtacacta agcccgattg
     6721 caaccggaac gacgtcctcg acgcctgcga actcctggac tgcgagccca ccaaccccgc
     6781 ctgccaggac tgcaacgaga acggcgtgct ggacgagtgc gacatcgcct ccggctactc
     6841 gcaggacgcc gatggcgacg gtcgcccgga cgagtgcgcc gcgccgtgca aattgggtga
     6901 ctcaaattgc gacggcaccg tgaacgtctt cgatatcgat ccctttgtca ttgccttgac
     6961 cgatcagccg gcctgggaag ccgcctattc ctgcggcttc ctctgcgcca atgactgcaa
     7021 tggcgacggg actgtgaatg tgttcgatat cgaccccttt gtgcaaatcc tgaccggaag
     7081 caactaagcc cgtcaggcag ctttgttgaa taaacgcgcc ggccggagaa cacccactcc
     7141 ggccggccgt ttcattttgc ggccaaccgc gtctgccccg caatcagccc ggcgtaaccg
     7201 gcagcggttt gatccggatg ttgcgatacc aaatcccgcc gccgtgatcc tggaaaccga
     7261 tgtgccccgc acgcggcata tccttgtagg ccgtgcggaa cttgttcggg ctgccgtccg
     7321 ggtttcgccc ggcttccttc caatcatcca agttcatatt aatgattacc aagccgttca
     7381 tgaccgcttg aaggcgcggc ccgcggcaca ccaggcgcag cgtgttccac tcgcccgcgg
     7441 gcttgacggc gttcttgctg ggcgcaaggc agtcgtaaat cgccccgcag tcgtgcttgt
     7501 cgacggctgc cttgccgtac gagtcgagca cctgcagttc gatgccggtt tgtacgcaat
     7561 cgtttatgtc ggccgtgcga aagaagacgc cgctgttggc gcctttatca atcttgaact
     7621 cgaggtccag aatgaagtcg ccgaactgtt gttcggtcca gatatcgccg ccgccccggc
     7681 gagccagaac tccgtcttca gcagcccagg acccgggctt acagatccag ccgctcaggt
     7741 cctttccgtt aaacagcgtg atcacgccga gcggcgattc gccggttgtg tgcggcaccg
     7801 gctcggcgtc ggcgctcagg tcgcctaacg cgaactgtac gccggccaga taatgcgcca
     7861 aaacctgcgg gttccaaaag atctcatcgc gatgcccgag cgagcagtag aacacgcgcc
     7921 cttttccgta ctgccgaacc cagctaatcg caaaatcgtc gtcagtgcgt ttgatgcccg
     7981 gcttggccgg atcgattttc gtcggctcca gactgagcag aacgcgcagc ttgtcccgcg
     8041 agtacggggc gccgaactga tagatttcgt cggcgatcac gaaaggcttg ccgtcgaagg
     8101 ccgcgttcaa cgggtggttc ggttcgtcca gccgaaccgt aaccggctca ttccacggat
     8161 gcccgtcgaa gtaaccgccc atcatttcgc catattcggg ccacttgtag aagcagtcgg
     8221 tcgcggcatg actgccgatc aggcccttac cgcctgacac gaagtccagc agcgacttct
     8281 tgagcgcttc gtcgcgccgc cgggccgctt cctgctccgc cgccgggagc tgttcgaaat
     8341 tcgccggccg gaacagctca ccggtgcagc tcatcagaca aatcgcgtca aagcgggcca
     8401 gcttctcggg cgtgaacata tcaatgtcgt cgctctcgac cgccgtaaac gcgcccgtct
     8461 tttcgcccaa tattttcatg gccgcggcgc cggtcggaat cgagtcgtgc cgaaacccgc
     8521 ggcagagcgt gaaaaccagc aaactccgcg gtttctgcgc ctgcgccgga gcgcggcgcg
     8581 gggcggcctc gctgattctc atcttcactt cgtctgtcac ttcaccgcct tgagcgcggt
     8641 aagcgctcat gcccgccaac agcagcgaaa tcgcgccaac caatatgatt cgcacttggg
     8701 atctcctggg ctcatgcggt aacctggccc ggactcgccc gcggccacaa cctgagccgc
     8761 actgcttgaa gctgcggctt caagcagtgg cacacagcag gtgtgtccat caacgccgcc
     8821 acggcaccgc cggagcgtgc cgaaaatcat accgattcac tggcccaagt cgatcttcag
     8881 gccctcgacg tgtgagccgt cgggccggcg cacgcgtgcg cacgccgccc agccgccgcc
     8941 gccattgacg accttcagca gcagctcgtt gcggcccttc ttcagttgtg cgggcacctt
     9001 atcctgcccg cgctcgcaac cgcgcacgcc ggcgaacgtg tgaatcagtt ccccattgag
     9061 ccagactttc agggcgtcgt cgctgccgag ctcaagttga acgtcctgcg cccggggcga
     9121 gtcgaggacc gtgcgcagat aagcagcccg attcgacccg gccatgtctg cgcaagcgtt
     9181 caggtccaca taccagaact ccggccggtc agtgggcatg gtctgcctct gccaggttac
     9241 gccgccgatg tccggctgct caggcggaaa agcgacctcc aacagctcca ggcagccctt
     9301 accgtcctgg tcgaatggcc cggctacttg ccaatcagtg atgaagccct cgtattcctc
     9361 ggcacgcgcc agcagcgctt tgccgcgctc tctaagggcg tcgtcatcca ccgcatcgag
     9421 cgccagccag atcgcttgcc gagcgcgctg ccaaccggcc gggagctgac gttcggccac
     9481 gcccaacagc gccgccccag cctcgttgcg cagttggtca tcgaggagga agcgctgcaa
     9541 cacggagatg gtgcgcggct ccggcaattc gccgatcttc gacagcagca acctgcgttc
     9601 atcgacccgg cgcgtgtgcg ccatgcactt ttcgtaggcg gcgagcttgt ccgccgcgga
     9661 cagctcctgg tccgcgccga ccaggcgcac gtacgcccgc agcgccagaa cgtgatggcg
     9721 cagctcgccc gaaccggtgg cgacttcgag aatattgccc gcggccgccg gatccggcca
     9781 ggtggacaaa gcgcggaaag ccgcttcgac gacttcagac cgcgaatcac ttaacgcttg
     9841 ccggaccgtc tggaaagctt cggcgccgcc caacttgcca agcacacgca gcaacgagca
     9901 gtaatcttcc gctcgcgccg ccggcagggc agccagaagc tttccggaga cggcaacgcg
     9961 gtccgcggcg cgccggcaca cggccgtcag cgcgctttcg cacgcctcac gctgctcgga
    10021 attctcgagc ttcggcaaca gccttaccag cggatccaac gcgctagacg gtgcgagcgc
    10081 ctcgaccgcc cgtagtgccg ccagccgcac gccctcgtcg tccttttcca agcagccaca
    10141 gatcgcgccg gcctgctcca cggcgtgcga atcagccaat atttgcagca gcctggcctg
    10201 tacacgcggc gggctcccgc tgagcgcacc ggcaatctgg gccgccgcct gctcatctcg
    10261 gatattcgtg agagcccgct gcgccgcagc gctcaagtcc ccatcgtcgc tcagcagctt
    10321 gatgagcggc tcgaccgccg cggtgcgccc cagcgccgcg gccgcttcca cggccgtcag
    10381 gcgaaccgcc ggcgattcgt cctgcagcgc ttcgaagacc gcactgagag cggcggcgtc
    10441 gccacgcttg cccagaatgc cgatgagagc gacgcggccg gtttccgtcg ccttgggcag
    10501 cctctccagg taggccgcgg ttacaccctc gccttgcaga gaaatggcga tattcgtggc
    10561 cgtggcgcga acctcggcgt cctcatccgc caggaacgag agcaccaaat cgatcgattt
    10621 cacgccggcg gcgctggaga gtgcggcgct gcgaatatga ggatccgcgc tttgcagttc
    10681 tgccgtcaga gcggcgtcgg cctccggctc gccttgctcg gccgcgtgcg ccgaggtgcg
    10741 gaatagcaaa gcgtccaact gtgaccgttc atagtgtgtc tggctttcaa acttgcgggc
    10801 cgcttcccga aacgccggcg actccatagt ggcgagcgct gctaaagccg ccaattgaat
    10861 tccgcggtcc ggcccgttgg cgtcttccgc gagccggtca gccgattcgg aatcgcggag
    10921 cactcccagg gcctgaatga tcgcaatccg gcacggcccc tgcgcctccg gataagccgc
    10981 gcgtaaagcc gcccgcgccg catctgagcc aatcgtaacc aacgcctggg ccacgaagtc
    11041 atagacctcc gggtccagca gaagtttccc caatgttgga accgcgctgt cgtcgccaac
    11101 gagctgaagc tggcgaacga ggaacgcctt cacccccggc tgtcgatctt tggacagagc
    11161 cgcggtgagc acctcgatgt acgcggcgcg ctgcgccggc gtgccgctgc tgccggtgta
    11221 ccaagccaga ccttcgaggg ccatgcgcgg cttggtgtca tccccggcgc ccgcctcgac
    11281 cagcatgtcg cacaaggtcg cccagtccga cggcggcagc tcagccagcg ctttgcccag
    11341 cacgtccggc ttggccgcgc cgtgaaagtc gatcctgcgc aagagccgct ccagttccgc
    11401 actcggcgcc gccatcgcac aggccgcaag cgccgtcaat acaatcgcgc atcggagcag
    11461 ttggcggggg gcgtgattcg tcacgcgcgc cagtcggcac gaatcgggct ttatcatctt
    11521 tgcctctcct acagatgcca cggggcgcgc atcgggactt cgatgagacg attggcctgc
    11581 tcgtcgccga taaaccgctc ctggtccggg tcccacttca gcggccgccc gaggcggatc
    11641 gcgatcgaag ccaggttgag caacgtgcac gagcggtggg ccacttcttc gttgccaccg
    11701 gccttctgcc gcgtttttac cgccgtttca aagtcgatca gcggcggcgg gtccggcaca
    11761 cgttcgagct gttcgaacaa gccgggcgga tcggtgcggt agtttcggta gactttgccc
    11821 ttcggccctt caatcagcgg caagcccggc ggctcaggcg agccccattc gccgctgttc
    11881 atgtagatgg tgtcgccatc cgcgtacttc agcgttaccg actcccaaac tccgactgcg
    11941 tcgggatgcg gcggccaagg ggctacggcc gaaacctcga ccgggccggt gtggtcctta
    12001 cccaggaagt actgcactgg gtcaagataa tgctggccca tgtcggtcaa tccgccgccg
    12061 tcgtagtccc agtaaccccg gaagctctgg tgtactcggt gcgggtgata cggcttgcgc
    12121 ggcgccgggc cgagccacat gtcgtagtcg agctcagccg gcacgggctc cggcgtcagg
    12181 ttggtccgcc cgctccattc ctggagcttc cagttgaagc cctgccccgc tccgacccgc
    12241 accagcaacg gcttgcccag cagcccgctg gcgaccagct tgcgcagcag cttcgtagca
    12301 ccgtagtcgt agtactgctc gaagcgaaaa tgcgtgttga tctgtaggat gcgcccgtag
    12361 cgcttgaccg tttcaacgta tgcccggccc tccgcgaccg tgtgcgaaaa cggcttttcc
    12421 gtcagaatgt caaagccggc ctgggccgcg gcgacgccca tcacggcatg ccagtgcggc
    12481 ggagtcgcga tgtggatcgt gtcgatgtcg gtacgatcca gcacccgccg ccagtcgctg
    12541 taagcttcac aactgcgccc cgccttggcc atggcggccg ccaggtgatt cttatccacg
    12601 tcgcagactg ccagcgtctg cggcggttgt ccctcgacgt tttcggtcac atggccggcc
    12661 atgcccattc ccccggcccc gatgacggcg cgcgtcagca cctcgctcgg cggcgtgtac
    12721 ccgcgtccgc cgagcacgtg ccgcggcacg atgtaaaaca ccgccccggc cttgaccgcg
    12781 gttccgagca gtttgcgcct ggtgagtttg atcgggcgtg gcatgcgtct cacctctccg
    12841 gagtttacct ttctcagtcg tcagtttttc agtcgactga taaccgagcg cgcgtctcgc
    12901 ctctccgtca ggcggcaacg cgcacgacaa tcatagcgcc gcccacggcc gtagcctagc
    12961 agcatgacag agatcaggga tcgagaaatc agggggggac aaaaccacaa cgcgccttga
    13021 gagttcggat caggaaagcc ggagggcgca taacgcggat ttcaaaccgg ctgcatcacc
    13081 cactgtggct cacgcccaaa tgtctccagc cgcgcacccg gtggcgagtc caggcagagc
    13141 atgccggtga tctgcgaatg gcggcggcag tagttggcca cttcgctcgg cgacaaaatc
    13201 atgaacgcgg tcgaaaacgc gtctgccttg gcggccgtgg ccgcgatcgc ccacgccccc
    13261 agcgcgccgg tgttgggggg ccgcccggtt tgcggatcaa taatgtgctc gccgtgcagc
    13321 aacatgccgg aaccgctgaa ggcctggtcg cgcaggtaga agcgcgccag actgctctcc
    13381 ggctggcgcg ggtcgcggac ggccaccgtc cagcccggct cgccacccgg cgcgcccagg
    13441 gcgaacgtcg tactctgacc ggcgtgcact agcgcggccg tgatcttcca gtcccgcagc
    13501 acggctaccg cctggtcgac gccataaccc ttgcctattc cgcccagatc gagcagcacc
    13561 gattccgaaa gcgccgtcac cgtccggctc tcttcgtcca ccccgatgcg cggcacgcgc
    13621 cgcgcgggaa cccgatgcgg accgggcccg agcaggctgc cgatcgttac atcaaacgcc
    13681 ccgtgcgtgg ccgcaccaac ttgcgccgcg atttccaagc aggcaaacgt gtcgggcccg
    13741 atacgcacgg actggcccgg cgcaagcgcg ttgatgcggg caatgtcgct ggtgggaatg
    13801 taacggctaa gttcacgctc gacccgatca acctccccga aagcggcctg cgcagcctgg
    13861 ccggcgtagc gctcatcgtc gcccacaatg aatattcccc agccgcagtt catcgcgcga
    13921 tgctcgaaca cgtgaaccgg atcggcgaga aggggcgagg tgccggtttg cgggtcggcg
    13981 cccatcgcaa aactcattcg cgttctctca ctggcccagg cgcgaggtgc tcttggcggc
    14041 gagcaaactc ttgccacaac tcgcgctcga catacgtcac caattgtata tcgggccgta
    14101 gaccgtagaa gctgattttg tagtcgtttt caaaaccggc cggcaggctc gccgccaggt
    14161 cgccggcgac gatcaccact ggagcgtcca actccgccgg cggcgcttcc cagtagccga
    14221 cacgctcgaa accccgcaaa taccacggca gcggccaggc atcttcagcg atcaccttga
    14281 ccagcatgcg gtacccatcc ggatgcacgt cggccagttt ttcgaggtaa gctgacaggt
    14341 acttcacgtc ccccagcgga tgagcgtaaa cgtaaggatt gcggtaatcg acgaagaacc
    14401 ggaaattggc ccgctgcgcc tgccagccaa ggtgcgcagc gccggccagg agaatcacgg
    14461 cggccaccgc ttttgccggc cagggcttga cccaggagat gatccccacc gcaccgagcc
    14521 cggccagcat gatcagcggc tgcacaaatt gcacggcgca ccacggcgtc ttatacggaa
    14581 taactgagta gacgaccgtc atcagcaccg cgtataccgc gatgaagcgc accagcccca
    14641 gccgccgcgg ctggtccgag agcccggcac cggtcacggc cacgccgatg ccggccagcg
    14701 ccagacccaa aatcaacccc tcgctccacc gcggcccgcg cgccaaatgc gtgaacccca
    14761 gcatttgcag atagtaatac cacgggtgat cgtgcagccc atggccgccg gcgcgctcaa
    14821 agtacgtcgc gtacgtcaac actgaatcca gcggcccacg ccagtgccga aagaacgccg
    14881 agtagaacag aaccgaaacc aggatcgcta ccccggccgc gactaccaag tcgccccaca
    14941 ccacgacgtt cttcttgatc cctcgatccc tcgatccctc gatccccttt tcaggttctt
    15001 ccccggccct ttcctttgtc ccgctccttc gctgccaaac agcaacgacc cccagcgccg
    15061 ccgccatgca ccccaggctg atgatcgacg tctctttcgt cgcatgcatc aggcctaccc
    15121 ccacgccacc caatagcgcc caccccggat tgcccgtctg ccagtaccgc cagcccgcgc
    15181 agatcaccag caaggcgaaa aacgccagca gcatctcctg tatgtagtac cggctgtaaa
    15241 acgtcatgat gggcgaaacg gccgtcagcg ccgccgtcgc gagcacaact ctccacccca
    15301 gcccatccag cagcaacaat aacagcgcaa ttatcccggc gccgaagagc gccggcccca
    15361 cccgcagtgt ctgctcgtcg agttccgcca gactctgcgc cccgctcagc cacacgaacg
    15421 ggaacgtggc gtagtacagc accggaccgt ggtactcgtg cgggtcatac acgtaatcgc
    15481 cggtctgcca gagcgtctcg aatttgaccg cgtgtaccgc ctcatcgccg tgcatgggcc
    15541 gcagctccag ccgcggcagc cgcagcgcca agcccccggc cacaatcagc gcgaaaatca
    15601 ggcgatacag atgctcaggt ttcatttggc tggccgggcg taaacctcga cctcaatgta
    15661 gtggttctga tcgtccgcgg tgctgccttt actgtacaga cgaacgtagc gggcgaccgc
    15721 ggccttgccc tcgatcagct tgccttcgta cgtctcccag tactccttgt cgcgccccag
    15781 ccccagaccc gaggaattat cgtggtcgtt gttgaacagc gtgcgaacgt tcgtgatgaa
    15841 atccggatca tccgcgatct gcaccaccac gtcgtggtac acccgcgcgt tggcgtgata
    15901 gtgccacacc aggatggccc atatctcggc cttctccttc aggtcaatct gtacccactg
    15961 caagcccggg cccagttcga cgtagctgcc ctcgtcggcc gaattgtcgc cgtccgtcac
    16021 cagcttgatc gagccaatga tcggttcgct atcgcttgaa gtcaccggct tgcctgcggc
    16081 cacgttcaca gcccctttgg ccgccatgaa cggcgggcgc ggccccttgc gcggctcctc
    16141 caggttggtt cccggcggca cgtgcttggg cgtgcccctg aacgccggct tcggcagctt
    16201 gatattgagt ggaaccaggt caccagccgc cttcgactcc ttcgcaacct cgccctgcgc
    16261 cccggcggcc ccgggcgccc cgtcatcggc cagtgataga accgctgcaa ctatcagcgt
    16321 cgcgctcaag gcggccggca gccgcgacag cttgctcaat ttcatcatcg atacctctgc
    16381 gccttccgag aaggaccgag acaaccaagt acataaccaa tgcgataacc agtccgattc
    16441 cggcacgctc gtgcaacagg cttagcgatt tctgcccggc cgtgccgaat agcggcgtca
    16501 tcgccgccaa cattgcggtc atcgtcacaa atccgcaagt cacgctgagc cagtaaacga
    16561 ccgcctgcgt tttctgcagg gcggaatttg cagaccctgc ccaacagcga cggcgggcgc
    16621 tgccgaagta ggccgctcca gtcactccca gaatcagcag cggagcgccg agcatgtgga
    16681 tcagcagcgc ccagccctca gttcgcccca gcgtccattt cgttcccaag cccgtaacca
    16741 gttgaacgac cagtccaacc aagcccaagc cgagcaccag cttttccatc cgcgcggctc
    16801 gaacccccag acgcccatcg ccgccgcccg ccgaccccac gttcaagacc acgcgcaaca
    16861 acagcaaggc cacgaccgcc gcgcacgcga atccaaacca cttgaacgcc ggccgaaaga
    16921 cgaaacccgc cgcccaagcc tgcactttcg cgccatcctc gccgcggaaa cgatacatgg
    16981 tctgcaccgc ctccggcccg gacggcacgc ctgcggccgc aatcgacccg taataaatcg
    17041 gtgccccctg tgcatggcag tccgtacagc cgccaatgcc cagcgcttgc gtggccggcc
    17101 gaacatcgtg cgccagtgac caacgatacg gcgcggtcgc taactgaatc gcgtttgcct
    17161 gctgcggcga aagcccgtgg cttccttctt ccgccggcgt ctcaaacgcc cgcttctgca
    17221 attcgcccga aggaccgcgg cgataaagca ggccatcacg cacgtatacc ggcgtatgcc
    17281 ctgcgtcagt cttgcctagt tgttccagca ccgcgccgat ctgctcttca gtcagcgccg
    17341 cgagcttttc ggcagacgct ttggcgcgct tcggatccac ctttttgagg gttttcagga
    17401 ctgaatcgag ggcataggct tcgactccct ctccggtgag ccgcccccaa tacgccggcc
    17461 agattaggcg ctgcggtgca agcttgccat ccggctgccg ggcgaagacc gggccgacga
    17521 tgttgggcgg cgtatccgca tcacgctcgc gcgtcgccag tcccaagccg tgcgccagcg
    17581 aagtctggaa ccggtgcgca tccatctccg gccagggacc cgaatggcag gcggtgcacg
    17641 taagcttctc caggtgcacc ggcggcaagc cccagtgctc cgggcgcggg gccgcatagc
    17701 gcccgccctg agccagctct gcctgcgaag ctccttccac ccctagatga caacccgcgc
    17761 aggaaaacgc cgccagcccg gcatcggcgc gctcaccggc ctcgccgtcg taaccgcgcg
    17821 ccatcatgtg atccatgccg ttgcggtggc agtcgacgca gagcaagccc gccgtcaagt
    17881 gcacgtcgcg gcaggagcgc tcttccgccc tcgctttcgg acccacctca cgcaccgaat
    17941 ggcagtaata gcaacgctca ttgggcggcc gccgcacgat gttgaacagt acgcgatcat
    18001 caacgtcgaa gcgcgatttg ttccaaacca tccgcgggcc gtctcgctcc gggtggtctg
    18061 ggctcggcgg caacgccgga tcccagtcat ccggcgcctt gcgtgcctcg ccgcgtatcg
    18121 ccgccaggcc taacgccgcc gtcgccgccc acttgaaatt ctccgcctcg atctgcacgg
    18181 ccgcttcggc cggatcgtgt tgcaggtccg cgctgtggca gaacatgcaa tcgatttcca
    18241 gcggcccgga aaccgcccag cgcgccttct ccggcgcact acgcagctca tccgcgtccg
    18301 gcatcccgta gcccccaccc ggcgtatgcc gcccgaagcg cttgatgaat ttccaggaac
    18361 ttagcccctc gtccgccggc ctaaacgtgc ccgcccagcc acgcccggag atcggcactt
    18421 gcgtgccgct cggcaaatca accagcagcc aggcctcgcc gggccgcccg gccgccgccc
    18481 cgccctgctt ggcattgaag tgccagcccc cggcaatctc tccgcacggg tggcatttgc
    18541 cgcaggtcat gcgcggcgaa taaggccccg cagactcatc ccgtggatcg atcgcgacgc
    18601 cgttctcatc gtacagcgtc agacgatgga cgtacggcgc ccggctgccg gtcttcagtt
    18661 cgttttctct gccggcctcc tgaccgaacg ctcgcggcac gcctgccaac ggggccagga
    18721 tagcgaccaa gccgatggtg atgccgttta ccaggcgcat gcggacagtg agcgcgttta
    18781 ccaatggcat gcccgcgctt acagcttggg catggcaccc acgcgcttgg gcatggcgcc
    18841 cacgactcgc cctctccgcc aggcacccgt gtacaaactg ccctgcaatc agaccttgaa
    18901 ctcctccggc ttgaactcgc agcgtttccc gctctccacc gcttcgttcg ccttcaacac
    18961 cgatacgcaa acctcgtacg ccaaatcggc cgggcaactc agcggtgagc cggtccgcac
    19021 ggcggtgaaa aagttctcca ggtgtaactg gtgcggcggc ttctggcttt cttcaagcag
    19081 ccgctggccc tcggggtctt tctcgccgct ggcggtcaac gtctcgccga tcttcagctc
    19141 gatcgcttcc ctgtccatca gctcgacctt gtcggcctcg ttctcccact cgcggcgctt
    19201 ggcggcctgc tcgcggaaga tatgcccctt cttcggatct tccgagatga ccagcgtgcc
    19261 ttcgtcgccc atgaacgttt cgtagtaccc gccgttgctg gtcgtattga gaacttcgta
    19321 gaacgcacgc accgtcccgg cggcagtctg gtactcgtaa atagccatca cattgtcgta
    19381 ccagtcgcgg ccctcctgct cggtgtagta atccagcccc ccgctggcca gcactgatct
    19441 cggcacggtc ttgagcaccc agttgaatat atcaatctgg tgcgagccca aatcggccat
    19501 cgggccgccg gagtacttgc ggtaccagcg ccaattacac agccgctcca ttgtgtcata
    19561 gccaaaacgc ttgagcactt ccgcgtccag ctcctcacct ttcgggcagc cgatctcata
    19621 aagccgcggc cggttccact ggccatagca gtgcgtgacg cgccccagga ttttgtcttt
    19681 ctcgatcatc ttcagggcat gccagtaacg cggattgctg cggcgctggt gcccaatctg
    19741 gagcagcttg cccgtctcac gcatcgcccg caccatctcc tgcgcggact gcgtcgtatt
    19801 ggccatctcc ttttcgcagt acacatgctt gccggccttg aggcaggcga tcgtgtgctc
    19861 ggcatgcatc cagtccggcg tcgcgacgat caccgcatcc agatgctttt ccttatccag
    19921 catctcgcga taatcttcgt aaccgttcac gacctggtcg tacttcttta gcgtgttaat
    19981 ggcataccgc cggctgtacg gccagatatc gcacaccgcg acaaaacgga cgccgggaat
    20041 cttgaggcag ttgagcagca agttccgacc ttggctgccc gagccaatga tgccgaccgc
    20101 cagctcatcc cccgcggcct cgctcgccgc cgcgcccccc gccgcctcct tcggcgcctg
    20161 agcccaggcc ggcatggtca tagccagccc ggccgcggcc gcggtctgca cgaactctct
    20221 gcgcgtaatc ccggattcgc tgtttcccgc ttcgcccatc atgttttgac ctctcggtag
    20281 gctccgtttc gcccatccct ccgtgcaggg ctactctatc gctgtaactc gtaacccgtc
    20341 aaaccgtgcc ccgccttgct cggataactt cggggatagc tcagaagtct tcttcacaaa
    20401 ggaccctcga caaccgcccg cccgtccgca aaaatccgcc tcagccgagc cgaaacttga
    20461 gccggtccgt cgggcgccct gggctgccct tgaaggagat gaacgatgcg ccgcttcaac
    20521 gcctttgctg tactgaccat cgcggccgtt tttgctgtcg cggcctacgc tgcttcgcgc
    20581 caaaacaccg ggcaaccggc ccaagccgga caaccggcca aaccctcgca atcggcccaa
    20641 aacgcgcaac cgggccgaaa cgcacaaccg gtgcaaccgg ctgaaccagc gcaaccggcc
    20701 aaaccactgc agccggcgca accggccaaa cccgcgcaac cggcgaattc gcccgctccg
    20761 gccgccagtg cgccggctca ga
//
LOCUS       DAOSYS010000033        20760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2100_length_20760_cov_5.351558, whole genome shotgun sequence.
ACCESSION   DAOSYS010000033 DAOSYS010000000
VERSION     DAOSYS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20760
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_20760_cov_5.351558"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..3123)
                     /locus_tag="PLU99_03465"
     CDS             complement(103..3123)
                     /locus_tag="PLU99_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16146.1"
                     /translation="MRAAALDNGDAIKPLIGEIRARMEELVARELALRERERDLERQF
                     RLLQGQHRQGAPPGWVESQERLRQRCAALDAQALELSARAEHLKELEARLLDREASLA
                     QAQRELSEQADRVRHRQDTLLKLRATDRENLRQRITRIRQRERDIENRVRLAHAEIVQ
                     QREKLEQQRQQLQNQALAIERAQEALRARTTALERETAQLEQRLAALDRQQRELQAQH
                     AQVAQHRAELAQTERALEQKQQELEQRARQLELEAASSAVQRDDFSRELDQICAQRTA
                     LIKAQAEHEQRLRRLADYEEQLRARAANLETETACLAETEAQIEARRRELDDHTQRAL
                     EDAQLQREQALALREQAEARDAEVRQRQLAAELELQELAAQRAALERSAVESEQRRRR
                     EEDHIAQARAALSNEAAEMRAAQHSALVRPGRWWLRTGVLSVLGAGIAAFAWFSLERP
                     VYEAAAELQLPPGTEPRFALAHEQRLRTPNLIEDLLDGHAFSPAWRQLRAAGRVRIEA
                     AADAPLIRITTRGSQPELLQPLVESAAARYAVQSSAGIESAEVAETIAQLNTERAALE
                     NQMRAAQQKQTSRENTVATLATEEQRDQARAASDQLRRDYELRSKNLVEAQSRLVDLE
                     KMPVRRGNVLPADYENALLADEMYQADLKEFHAALVEYRSEMRVAMLLLSDPLKNLEG
                     ALSDTAAVVAEQRGLQPPPQVAAVLEDFESKLAAFQKRLEEFVVEWEAGAAQLRDVDA
                     TVELADLINRQVAAADQARQFCGETKGLENELHARLEQFTSGSEGGTREVVVVAVLRS
                     ELAKLNARNAELTSAADNTDATRNVRLDALERQARGLQSRLERRQVSVRQLLQFEADE
                     RARVDQMEQLSALRQNVQELEEARERLIRDFTAGLDELRRLEDEVVQRRILTAEAEQA
                     AAEIERCQSRITQIDGQIVAAQKGGLLPPVQAGGAQVRTIAGQYRTRNALLAGLGTAA
                     AVYAVSFLTLVRNPLRRRRHWETVLKSEPTDAAN"
     gene            3438..4112
                     /locus_tag="PLU99_03470"
     CDS             3438..4112
                     /locus_tag="PLU99_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16147.1"
                     /translation="MLSGLAECRNLLVRRWGEMGGYWGINRTMAEIHALLFVSGEALC
                     TDDIMAQLHISRGNASMNLRGLVDWGLVQRQHRLGDRKEYFQADGDVWHMFETIMRER
                     RRREVEPILNAIERCRAIMSSPGQPATAQGEDFLRRLDELRRFLSTMGTLFEMVVQFG
                     PDGIDGLLRLAGAGVPAGRPASGQPRTLETRPVAAPAGGRPLSQNGRAGDKRHKSGRG
                     GRKARI"
     gene            4147..4680
                     /gene="pyrE"
                     /locus_tag="PLU99_03475"
     CDS             4147..4680
                     /gene="pyrE"
                     /locus_tag="PLU99_03475"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOO16148.1"
                     /translation="MTDAELAQALRQAALLEGDFTLRSGRKSKYYLDKYLFETRPELL
                     RELALRFARFVDQNTDRIAGAELGGIALAAAAALETGRPFVIVRNSKKAGYGTGKLIE
                     GRLNPKERVLLVEDIATSGGQAVEAINTLRASGAEVTGVVVVIDRQEGGRQAIESAGV
                     AFHALFRSEDLGIRPEG"
     gene            complement(5249..6070)
                     /locus_tag="PLU99_03480"
     CDS             complement(5249..6070)
                     /locus_tag="PLU99_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16149.1"
                     /translation="MVPIILFSLVHTILALSHAQPNTQPVPLEEPPPATQPANERSAA
                     QVLRISLPDVRLEKASLERALIWLAETADINISVNWNRLAAFGIRPDTPVSLAGTQLT
                     VGSYLDLLIADVNREKDKLGYEVGRNLIRIAARQDLDDELVTRTYPVHDLLQSRQGRP
                     AQLFVGRLQDYVFTIDPFVAPGVAMPRPVTARAASAAFIGMADPPARTGESAEEERER
                     LAAELIAAITASVEPDTWEVNGGRGTLSIFEGTLIVRNSQRVQRILGSTLLPSAP"
     gene            6738..7616
                     /gene="ispE"
                     /locus_tag="PLU99_03485"
     CDS             6738..7616
                     /gene="ispE"
                     /locus_tag="PLU99_03485"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOO16150.1"
                     /translation="MASLTRLSPAKINLTLRVTGLRPDGFHEIESLVALVTLHDSITV
                     VRRADGKRVLRCDDPRLPCDERNLALRAAAELAKECGVTGGFELVLQKRVPMGAGLGG
                     GSSNAATTLLLLDELWGLHLSRERLTQIGARVGSDVPLFLNGAVSIIRGRGEQVEAVD
                     VPVAGCVLLLMPDLHSATADVYRAWDSLAVHPPRIELSEVLTRADQPAEFSRVLFNDL
                     EEAALQVQPELGRLAGELRRILACPVHMTGSGAAFFCVLERTEARKAADDIRAAGLAV
                     RVEQVDFVASGGRATE"
     gene            complement(7701..9668)
                     /locus_tag="PLU99_03490"
     CDS             complement(7701..9668)
                     /locus_tag="PLU99_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HOO16151.1"
                     /translation="MKNETASPAAAPPQDSGRERSQSDQLEKPLPLSGFTGSPEEIER
                     QWYEQVYKNRGDSMAQLTWRAVLMGSVLGGILSLTNLYIGLKTGWGIGVAITACILSY
                     AIWTTLYRLGLARTRMTILENNCMQSTASAAGYSTGGTLVSAIAAYILINGHSLPTLT
                     LMAWVFFLAVLGVTMAIPMKRQMINIEQLRFPSGVAAAETLRALHSTGGGGMKAAKAL
                     GLAGLLSALNNLWCDGLHLISEKLAPFALAEILSRVNLAALGTNWVGRSVSFVWDPIF
                     IAAGAITGIRISASMLLGSVLCWVIFVPLLQHYMAQGGTIIGSQYKELINWTLWGGAS
                     CMVTAGLLSFALQWRSALRAFAGFGQMIFSRGRRSDDPLQRIETPASWFVIGQVVGLT
                     GLAYLAWTTFGVPVWITVVAVLLSFALALVACRVTGETDTTPVGAMGQITQLTFGAIH
                     PGSANVTLMTACVTAGAADSSSDLLIDLKSGYLLGANPRRQFIAQFSGIFMGTLVSVL
                     SFQLMVPDATALGTDRFPAPSAQAWRAVAVALSAGLAALGPVKTWSIVIGGLVGVALP
                     LLERLLPKYRKFIPSASGVGLAWTFPFYYGVLFFLGALAALLLERKWPKWAETYTIPV
                     ASGVIAGGALMGAALIFAENGPAIWRQLFGQ"
     gene            complement(10369..11013)
                     /gene="trmB"
                     /locus_tag="PLU99_03495"
     CDS             complement(10369..11013)
                     /gene="trmB"
                     /locus_tag="PLU99_03495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOO16152.1"
                     /translation="MSEQPLPPPAPAPPIPVHLDELENLRWSAFFGNDHPVELEIGIG
                     KGGFLLRRAQAYPQRNFLGIEWSNEFYRFAVDRMQRWNLRNVRILRADASYFVRAKCP
                     RDSLLALHVYHPDPWPKRRHHKRRLFQKPFVDAAAACLIPGGRWAVQTDHAEYFEVIR
                     ELLLNHPELEPIPFDDPDFGVAEARIATNFEIKYLREGRTMYQLAVRRIPQTKS"
     gene            11314..13431
                     /locus_tag="PLU99_03500"
     CDS             11314..13431
                     /locus_tag="PLU99_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16153.1"
                     /translation="MAASGALAQDRNYLIVSAPDYVGSAPLTQFINHRTSRGFNVSVY
                     SVPPGTSREDIKAHIQSLWGTPDAPEFLLIVGDTSGTSSTGTTIPHWVGQGSRHATTD
                     LPYACMDSGDDWYPDMFHGRFSVTSVNMLQQVVDKTICVESGNFSDPNYVRRAAMLAT
                     DDPTAGAATLHETIISSFLEPAGFTVTRVYAAAGGGTSHISAAVNAGVLFTVYFGHSG
                     SGGWSSPSFSQSNVNALTNEGLYGLTMGWSCNTAHYDYDECFGETWLRAANKGAAAYL
                     SASNYVWWSSSADWESSRRMENYFFKAVFRKEICEVGPAWQDALWTILGDPAFGPTHD
                     HTRNIFEQMVLLGDPALRLPLRALDLSLPNGWPEFIPPDVPTPIVLRIADAAQSYLPD
                     SGRLYYRYSGDEYQSVPLVPLGDGLYEGILPPPGCNANPEYYFSATGDQEDTVYLPEL
                     APQQVLTSIVATTTTLFTDDFEADQGWTVWTDPGLTTGAWERGIPLGTGQVGAPVADY
                     DGSGRCFVTDNRGGAYDVDGGPTQLISPTIDISGMSDVYVRYARWVYCDDELPPAQDF
                     LDIEFSADGGANWTAVEHVSGPGGWVRQEIRVSDFVPLTSQFKMRFSINDTPNNSLTE
                     AGLDAVVIYDRACGLEAMLGDLNCDGVLNVFDIDPFVLALTEPAGYAAAHPDCDANRA
                     DCNQDGEVNVFDIDPFVQLMTGG"
     gene            complement(13492..14571)
                     /locus_tag="PLU99_03505"
     CDS             complement(13492..14571)
                     /locus_tag="PLU99_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HOO16154.1"
                     /translation="MPLSPFFAIGLLLTSPPQPEPTLSADAVARFANLALACVHKDYP
                     YKIAHVLNSDADVAPPRELTPVFCGCYDWHSSVHGHWLLVRLCRMYPQADFVPRARAA
                     LAESFLPEKVADELNYLRGPGRKSFERPYGLAWLLQLSAELHEWHDPQAREWARTLEP
                     LEQVAVERFSEWLPKLTHPVRTGEHTQTAFALGLLLDWARSRGNREFAALVTSRALAY
                     FQNDRGANLAFEPDGQAFLSPILAEADVMRRILPPAEFAAWLSAFLPDLKGDESRDWL
                     PPAVVSDRTDPQLVHLDGLNLSRAWMLEGIASSLPAGDPRIATLQAAARAHRQAGLAA
                     LADQQYEGGHWLGSFATYLVTRRGL"
     gene            14813..16984
                     /locus_tag="PLU99_03510"
     CDS             14813..16984
                     /locus_tag="PLU99_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16155.1"
                     /translation="MKASRANCSYRGWVAAALTLALAASAVADKNYLIVSASSYVGSA
                     PLTQFINHRTARGFNVMVYSVPAGTSRDSIKGYIQSLWGTANQPEYLLIVGDTSGSTS
                     TATTIPHWVGGGSRQATTDLPYACMDGGGDWYPDMYLGRFSVTSVAMLQNVVDKTICV
                     ESGGFADPDYARRAVMLATDDSTAAAPALHDSIISQYLEPAGFTATRVYASSGGGSSH
                     ISAAVNAGVLFTVYFGHSGSTGWSSPGFSQSNVNALTNDGKYGLTMGWSCNTAHYDYD
                     ECFGETWLRAAHKGSAAYLSASNYVWWGSSGSWESSRRMERYFFQSFFVDNIWEVGPA
                     WQAALWKILADPDFGPAHDHTRNIFEEMVLLGDPALRLPFRALDLLLPDGTPEFIAPD
                     VPTTIRVRINDASELYVPGSGMLHYRYDKGDWLTVPLEPLGGSLYQGTLPAPGCDATP
                     EYYFCATGDRGGTACLPDNAPVHVLSSSVAVITTLLAEDFESADGWTVETDPSLTTGA
                     WDRGIPPGAGQTGAPPADYDGSGRCFVTDNRVGNYDVDGGPTHLISPMVDISRMQEVH
                     VRFAAWMHCDDALPPDRDYLDVEFSSDNGQTWVLADHIPSRDKWVERDIVVNNFVPLT
                     TQFRMRFTANDTPNNSVTEAAVDSIWLYDNACGPGLMPGDMNCDGIVNAFDIDPFVLA
                     ITDADAYAAVHPDCNIYAGDVNEDGSINAFDIDPFVLLLTE"
     gene            17368..18603
                     /locus_tag="PLU99_03515"
     CDS             17368..18603
                     /locus_tag="PLU99_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOO16156.1"
                     /translation="MTEIANSAARIAAAGRRGTDVRSDCWVQITLAESGGIDLQLKTK
                     VEVMYGQATRKLVHEALAALGVTNARVEIEDAGALPWVMLARIEAAVRRLDGRGVTDI
                     MAVPECLPEFVAGAQYPTERERFRRSRLYLPGNEPKFMLNAGLHQPDGVILDLEDSVA
                     PAVKDEARLIVRNALRSIDFRGSERMVRINQGERGLVDLDFLVPHNLHVVLIPKVESP
                     EQVRAVDGRIDRILASKRHGDAAGRRPFLMPIVESALGAIKAYEIATASPHVIALTIG
                     LEDYTADIGAQRTNEGRESFWARSQVVNAARAAGVQPIDTVFSDVSDMEGLRASVLEA
                     KGLGFEGKGCIHPRQIRPIHEAFAPAPAELEKAQKIVAAFKEAEEKGLGVVSLGSKMI
                     DPPVVKRALRIVRLAEALG"
     gene            18657..20045
                     /locus_tag="PLU99_03520"
     CDS             18657..20045
                     /locus_tag="PLU99_03520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOO16157.1"
                     /translation="MRWWRRYWMAVAALLGLAVWGLTDIRWRGQFRPDAPELHRTDFT
                     VYTNAADVLLSGGDPYAVPGPRGWYYLYPPLFALLVSPLRHLDSQWQVVIWFAISVGL
                     AYGCLSEGRRIGLWLWKRCAELPSAQVMQPPAWVGIVAFVTVLLPLLECLQRGQVGVL
                     ILLSLMLGLRLTLTRESALQVAGGGVLLALPAAIKITPALPVLLYAVLLGTTGPRWRG
                     AAAWGGLVAGTALFWVGIPGAVVGWDENLRLLARWRDTVAANEMLVSDRGINPLSWSN
                     QSLEVALFRLGEIAQHGFPPPLRAEQVGSAGVRLTPFPPRSAADWQTPISAGRIGLLV
                     LLLAAAVRLWRAADLSGLAAAFGLGCAASLLISPISWGHHFVLLFPAGLLVPAWCLLR
                     GRRRLAKLSAGTLAALVLIHYICMPVAGPWSVLGLGLTVWFVIVCVALIWAARRGGDT
                     HSTDTTQLASTA"
     gene            complement(20090..>20760)
                     /locus_tag="PLU99_03525"
     CDS             complement(20090..>20760)
                     /locus_tag="PLU99_03525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO16158.1"
                     /translation="PAGMPSVPGRNTPPSAIELLSRQPELSGHGGLRVVHRLDRGASG
                     VLVFARTLAAQRGLVQQFMERRIEKVYLALVSGYVAEDGEIDLPLYFNSRMQRAEVNH
                     SKGKPSLTRYHIVERLAGHTVLECRPITGRMHQIRAHLAAIGHPLGVDPTYGGRESIL
                     LSEFKPDYRVNRSGQERPLIERLTLHAARISFEHPISGAAISCEAPLPKDMRATIRQL
                     ARLA"
BASE COUNT         3552 a         6030 c         7225 g         3953 t
ORIGIN      
        1 caagcacgag gaggagggta gggggccgca gttggcaaga atgttcttgc aaatgttcaa
       61 cgagtgtgcc actgcatgaa gcgcagcttc aagcagtgcc gctcagttcg cggcgtcggt
      121 tggctcggac ttcaacacag tttcccagtg gcggcgtcgg cggagcgggt tgcggaccag
      181 ggttaggaat gagacggcat aaacggcggc ggccgtgccc aagcccgcta acagtgcgtt
      241 gcgggtgcgg tactgaccgg caatcgttct gacctgcgcg ccgccggcct gcacgggcgg
      301 cagcaggccg cctttctggg cggcgacgat ttgcccatcg atctgcgtga tgcgcgactg
      361 gcagcgctcg atttctgccg cggcctgttc cgcctcggcg gtcaggattc tgcgctgcac
      421 aacttcgtct tcgagccgcc gcagttcatc caagccggcg gtgaagtcgc ggatcagccg
      481 ttcgcgggcc tcttccaatt cctgcacgtt ctgccgcagg gcggagagct gttccatctg
      541 gtcgacgcgg gcgcgctcat cggcttcgaa ttgcagcagt tggcgaacgc tgacctggcg
      601 ccgctccagc cgcgactgca agccgcgcgc ttggcgttcc aaggcgtcca gccgcacgtt
      661 gcgggtggcg tcggtgttgt cggccgcgct ggtcaactct gcgttcctgg cgttcagttt
      721 ggcgagttcg ctgcgcagga ccgccacgac gacgacttca cgcgtgccgc cctcagagcc
      781 gctggtgaac tgttccagcc gggcgtgcaa ctcattttca aggcccttcg tttcgccgca
      841 gaactggcgg gcctggtcgg cggcggcaac ctggcggttg atcaggtctg ccagctcgac
      901 cgtggcgtcg acatcgcgca gttgcgccgc gccggcctcc cactcgacca caaactcttc
      961 caagcgcttt tggaatgcgg ccagcttgga ctcgaaatcc tccaggaccg cggcaacctg
     1021 cggcggcggc tggagcccgc gttgttcggc gacgacggcg gccgtgtcgg acagtgcgcc
     1081 ttccaagttc ttgagcgggt cggagagcag cagcatggcc acgcgcattt cgctgcgata
     1141 ctcaaccagg gcagcgtgga attccttcag gtcggcctgg tacatctcgt ccgcgagcag
     1201 ggcgttctca tagtcggccg gcaacacgtt tccacgccgc acgggcattt tctccaggtc
     1261 taccagccgg gactgggctt cgacgaggtt cttgctgcgc agctcataat cgcgccgaag
     1321 ttgatcggag gcggcccggg cctggtcgcg ttgttcttct gtggccagcg tagcgacggt
     1381 gttctcgcgg ctggtttgct tctgttgagc ggcgcgcatc tggttttcaa gcgcggcgcg
     1441 ctcggtgttg agttgggcga tggtttccgc cacttcggcg gattcgatcc ccgcgctgga
     1501 ttggacggcg tagcgggcag ccgcgctttc caccagcggc tgcaacagct cgggttgcga
     1561 gccgcgggtt gtgatgcgaa tcaggggcgc atcggccgcg gcctcgattc gcacccgtcc
     1621 ggctgcgcgc aactggcgcc aggcaggaga aaacgcgtgg ccgtccaaca agtcttcgat
     1681 cagattcggg gttcgcagcc gttgctcgtg cgcgagggca aagcgcggct cggtgccggg
     1741 cgggagctgc aactcggcgg cggcctcgta aaccggcctt tcaagcgaga accaggcgaa
     1801 ggcggcgatg cctgccccca gcacggacag gactcccgtt cgcagccacc agcgcccggg
     1861 gcggacgagc gcggagtgct gagcggcccg catttcggcg gcctcgttgg aaagggcggc
     1921 gcgggcttgc gcgatatgat cttcctcgcg ccggcgccgt tgctctgact caacggcgga
     1981 gcgttccagg gcggcgcgct gtgccgccag ttcctgcagc tccagttcgg ccgcaagctg
     2041 gcgctggcgc acttcggcgt cgcgggcctc ggcttgttcg cggagggcca gggcttgctc
     2101 gcgttgcagt tgggcgtcct cgagggcgcg ctgcgtgtgg tcgtccaatt ctctgcggcg
     2161 ggcttcgatc tgggcctcgg tctcggccag gcaggcggtt tcagtttcga ggttggcggc
     2221 gcgggcccgc agttgctctt cgtagtcggc gagccggcgc aggcgttgtt cgtgctcggc
     2281 ctgcgctttg atcagggcgg tgcgttgggc gcagatctgg tccagctcgc gtgagaagtc
     2341 gtcgcgctga acggcggatg aggcggcttc caactcgagc tgccgtgcgc gctgttcgag
     2401 ttcctgttgt ttctgctcca gggcgcgttc ggtttgagcc agttcggcgc gatgttgcgc
     2461 gacttgcgcg tgctgggctt gcagttcgcg ctgctggcgg tccagcgcgg ccaagcgctg
     2521 ctccagttgg gccgtctcgc gttccagcgc ggttgtccgc gcgcggaggg cctcctgggc
     2581 gcgctcgatg gcgagggcct ggttctgaag ttgctggcgc tgttgttcca atttttcgcg
     2641 ctgctggacg atctcggcgt gggcgaggcg cacgcggttt tcgatgtccc gttcgcgctg
     2701 gcgaatgcgc gtgatgcgtt ggcgcaagtt ctcgcggtcg gtggcgcgca gcttgaggag
     2761 cgtgtcctga cgatgccgga cgcggtcggc ctgttcggac agttcgcgct gggcctgcgc
     2821 cagcgatgct tcgcgatcca ggaggcgcgc ctcgagctcc ttcaaatgtt cggcgcgggc
     2881 ggacagctcg agggcctgag cgtccagcgc ggcgcagcgc tggcgcaagc gttcttgcga
     2941 ctcaacccaa ccgggaggcg cgccctggcg gtgctggccc tgcaggaggc ggaattgacg
     3001 ctccaggtcg cgttcgcgtt cgcggagggc gagctcgcgc gccacgagct cctccatgcg
     3061 cgcccggatc tccccgatga gcggcttgat ggcgtcgccg ttgtccagcg ctgcggcgcg
     3121 cacggcaaac tcctcgacgc tgtcgcttcg aagctctggg ggctggcccg cttgcgcact
     3181 gcgggcccga cccgtccctg gaaccgagct ttgttggtcc ggtaaatctt ccatcgggct
     3241 accagcggca ggcgcgatcc ccgccgccac tcacgccgcc agtgccattg taactatcgg
     3301 ttcaatggcc gcacctgtcg cggtaagggg cgcgggggcg gcatgaaccg cgcggcgggg
     3361 gcgcgggctc gtggcttgca atttcgcgcg aagtgattac cgttttagat agttctgaaa
     3421 cttttacgga gagaagcatg ttgtcgggcc tggcggagtg caggaacctg ctggtgcggc
     3481 gctggggcga aatgggcggc tactggggga tcaaccgcac catggccgag atacatgcgc
     3541 tgctctttgt atccggcgaa gcgctgtgca ccgacgacat catggcgcag ttgcacatct
     3601 cgcgcggcaa cgccagcatg aatctgcgcg gcctggtaga ttgggggctg gttcagcggc
     3661 agcatcggct gggcgaccgc aaggagtact tccaggccga cggcgacgtg tggcacatgt
     3721 tcgagacgat catgcgcgag cggcggcggc gcgaggtcga gccgattctc aacgccatcg
     3781 agcgctgccg ggcgataatg agttcgccgg gccagccggc gaccgcgcag ggcgaggatt
     3841 ttctgcgccg gctggatgag ctgcgccgct ttctgtcgac catgggtaca ctgttcgaga
     3901 tggtggtgca gtttgggccg gatggtatcg acggactcct gcggctcgcg ggggccggcg
     3961 tgccggccgg gcgtccggcc agcgggcagc cgcgcacgct ggaaacgcgg ccggtggcgg
     4021 caccggccgg cgggcggccg ctttcacaga atgggcgcgc gggcgacaag cggcacaaaa
     4081 gtggccgcgg cgggcgcaag gcgcggattt aggccattag gatttaggga tcgcaaggcg
     4141 ggtaacatga cggatgcaga attggctcag gcgctgcggc aagcggctct gttggaaggg
     4201 gattttacgt tgcgctcggg ccgcaagagc aagtactacc tggataagta cctcttcgag
     4261 acgcgcccgg agttgctgcg cgagctggcg ctacggttcg cgcgcttcgt cgatcagaat
     4321 acggatcgca tcgccggggc ggaactgggc ggcattgctc tggccgcggc ggccgcgctc
     4381 gagacgggtc ggccctttgt gatcgtgcgc aactcgaaga aggcggggta cgggaccggc
     4441 aagctgatcg agggccggct caaccccaaa gagcgcgtgt tgctggtcga ggatatcgcg
     4501 acttcgggcg ggcaggcggt ggaggcgata aacacgctgc gcgcctcggg cgcggaagtg
     4561 accggggtcg tcgtggtgat cgatcgtcag gagggcggtc ggcaagcgat cgaaagtgcc
     4621 ggagtggcgt tccacgctct gtttcgctct gaggacctgg gtattcggcc ggagggatag
     4681 aggaagaggc tcaggaatct aggcgcgacg ggctcaggga gcgaggggcg catccccgga
     4741 attctcttgg tcgcgttctt taaaatacct ggatactctg gtgcataatc aaagtgggga
     4801 gccaagctcg cgcttgactc cccactgcaa aggctcccct tctggctgtc cgatgggctg
     4861 cgccgcggtt ccactcgctg cttttccagt gcggccggtg ggatggggcg ccgaccttca
     4921 caccggcggt ctatggcgga attcaaggct cccggtcgtg agtcacgcgg ctagcgtgaa
     4981 gaacagagta cgagtacata cacggaggcg atctcacaac ggggagccat taattctggg
     5041 gcctctggcg cggccgaaac ggtctttggt tgtctacagc gctaattggc gcgcctgttc
     5101 ggcagtacga gcgtgacgca ctcctgcacc gccgtccgtg agagtatgca ttacaaaatt
     5161 aacgtggggc tgaatttcta atgacgttct tgtcaagtgg gaattgtgta ctcggaaaat
     5221 ccactcactg gtcaaatcca ctcactggtc agggtgcgct cgggagcagt gtgctgccca
     5281 gaatccgttg cacccgctgc gagttccgca caatgagcgt gccttcgaaa atggagagcg
     5341 ttccgcgccc accgttgact tcccaagtat ccggctccac cgaagcggta attgcggcta
     5401 tcagttcggc ggccaggcgc tccctttctt cctccgcgct ttcgcctgtc cgggccggcg
     5461 ggtctgccat accaatgaaa gccgcgctgg cggcgcgtgc agtgaccggg cgcggcatgg
     5521 caacgcctgg tgcgacgaac gggtcaatgg taaatacata atcctgaagt cgtccgacga
     5581 agagctgtgc tggacggcct tggcgagact gcagcaggtc atgcaccgga taggtgcgcg
     5641 tcactagctc gtcgtcgaga tcctgcctgg ccgcaatgcg tatcaaattc cggcccacct
     5701 cgtagcccag cttgtccttt tcgcggttca cgtccgcaat taacaaatcc aggtaactgc
     5761 cgaccgtcag ttgcgtgccc gccaggctga ccggcgtgtc gggcctgatg ccaaatgcgg
     5821 ccaaacggtt ccagttcacc gaaatattga tatctgcggt ttccgccagc catatcaagg
     5881 cgcgctccag agacgccttc tcgaggcgca cgtccggcag gctgattcgc agcacctgcg
     5941 cggccgagcg ctcgttggcc ggctgcgtgg cgggtggggg ctcctccagc gggaccggct
     6001 gcgtgttggg ctgggcgtgc gacagcgcga gtatggtgtg gactaaggaa aataggatga
     6061 ttgggaccat ggcgtgctcc tccgagcgcc ggtcgcacac gttgcgcccg tacgagttga
     6121 ttgtctcacg tccgcgggaa attgccagaa aaatcaggac gcaggggccg gtgcggagtg
     6181 ttctcgcgta ctgaagagat gacgagtcta agggtgccgg cggcgagcta tgccggtgac
     6241 gctgcgttgc ttgcgtctgc cctgggcatg cccgcgcttg gcgcttgggc atggcatcta
     6301 acccggccgg catacgggcg ttcagtaccc gacggtgggg tgcgcgaccg tcgagcaaga
     6361 acgttttttg cggcgcttta caattggtgg aaagcgcatt agattagtgc tatatcaatg
     6421 gtggtgttcg atagatgccg agggaaggag gagtgattcg gccgcgacgt ccgagtaaac
     6481 gaaggccgca aagccaatag ggccggcaat ggggccgagg cttggatgga tcgcgagcat
     6541 cgtgcgagtg ccgctacggt tacccgcgtc tgagtggcgc ggggtcggag tggaggatgc
     6601 ggtgcccggg ccagagtggc ccgggctact gcatttatgg gactatgcgt gtgaaagtcc
     6661 ggctgtcacc aagctgcatg gcgctttgca gatcgcgcat ttgacggttc gcgtcaaagg
     6721 cggcatgctc gcaggcgatg gcgtcgctaa cccgattgtc tccggcaaag atcaacctca
     6781 cgctacgtgt gaccggcctg cgccccgacg gattccacga aattgaatcg ctggtggcgc
     6841 tggttacgct gcacgacagc atcacagtcg tgcggcgcgc cgatggcaag cgggttttac
     6901 ggtgcgatga cccgcggctg ccctgcgacg agcgaaacct cgcactgcga gccgcggccg
     6961 aactggccaa ggaatgcggc gtcacgggcg gctttgagct ggtgctgcaa aagcgggttc
     7021 ctatgggggc gggtttgggc ggtggcagct cgaatgcggc cacgacactg ctgttgctcg
     7081 acgaactgtg ggggctgcac ttgtcgcgtg agcggctgac gcagatcggg gcgcgcgtcg
     7141 gatcggacgt gccgctattt ttgaatgggg cggtgtcgat tattcgcggg cgcggcgagc
     7201 aggtggaggc ggtggacgtg ccggtagcgg gctgcgtact gctgctgatg ccggacctgc
     7261 actcggcgac ggcggatgtg taccgcgcct gggatagtct ggccgtacat ccgccgcgca
     7321 tagagttgag cgaagtgctg acgcgggccg atcagccggc ggaattttcg cgagtgcttt
     7381 tcaacgatct ggaggaggcg gcgctgcagg ttcagccgga actgggccgg ctggcagggg
     7441 agctgcgccg cattctggcg tgccccgtac acatgacggg gtcaggggcg gcgtttttct
     7501 gcgtgctgga gaggacggag gcgcggaagg ccgccgacga cattcgcgcc gctggcttag
     7561 cggtgcgcgt ggagcaggtt gatttcgttg catccggagg gcgggcgacg gagtgataaa
     7621 gtgataaggt gatagggtga taaagtgctt ccgcagacca ccctttatca ccctatcact
     7681 ttatcacctt atcactgctt ctattggccg aaaagttgtc gccagatagc cggcccgttc
     7741 tcggcgaaga tgagggcagc gcccatgagc gcgccgcccg cgatgacgcc ggatgcgacc
     7801 ggaatcgtgt aggtctccgc ccatttgggc cacttgcgtt cgaggagcag cgccgccagc
     7861 gcgcccagga agaacagcac gccataataa aacgggaagg tccaggccag cccgacgcct
     7921 gaggcggaag ggataaactt gcggtacttc ggcagcaagc gctccagcaa tgggagcgct
     7981 acacccacca ggcccccgat tacgatcgac catgttttca ctgggccaag cgccgccagg
     8041 ccggcgctca aggcgaccgc caccgcccgc caggcctgcg ccgaaggagc cgggaatcgg
     8101 tccgtcccga gcgccgtcgc atccggcacc atcaactgga agctgagcac cgacaccaac
     8161 gtgcccatga aaatgccaga aaactgtgcg atgaactgtc tgcgcggatt ggcgcccagc
     8221 aagtagccgc tcttaagatc aatcagcaag tcgctggatg agtccgccgc cccggccgtg
     8281 acacacgccg tcatcaacgt gacgttcgcg ctgccggggt gaatggcccc gaaggtgagc
     8341 tgcgtgatct ggcccatcgc acccaccggc gtggtgtcgg tttcgcccgt gacgcggcag
     8401 gcgacgagcg ccagggcgaa cgacagcagg acggccacca ccgtaatcca gacaggaacg
     8461 ccgaaagtcg tccaggccag ataggccaga ccggttaggc cgaccacttg gccgatgaca
     8521 aaccaggagg caggcgtctc gatccgctgt aaagggtcgt cgcttctgcg gccccgagaa
     8581 aagatcatct gcccaaaccc ggcgaaggcg cgcagggcgc tgcgccactg caacgcgaac
     8641 gagagcagcc ccgccgtgac catgcacgag gcaccgcccc agagtgtcca gttgatgagc
     8701 tccttgtact ggctgccgat gatcgttccg ccctgcgcca tgtagtgctg cagcagcggc
     8761 acgaagatca cccagcacag cacgctacct aacagcatgc tggcgctgat ccgaattccc
     8821 gtgatggcgc cggccgcaat gaagatcggg tcccaaacga aactaaccga ccgaccgacc
     8881 cagtttgtac ccagcgcggc caggtttacc cggctcagaa tttccgccag ggcgaacggg
     8941 gcgagcttct cgctgatcag gtgcaaccca tcgcaccaga ggttgttaag cgcgctgagc
     9001 aggcccgcca gcccgagcgc cttggcggcc ttcatcccgc cgccgccggt cgagtgcaag
     9061 gcgcggagcg tctcagccgc cgcgacaccg ctgggaaagc gcaactgctc gatgttgatc
     9121 atctggcgtt tcatggggat ggccatggtc acgcccagga ccgccaggaa gaagacccac
     9181 gccatgagcg tcagcgtggg caggctgtga ccgttgatga ggatgtatgc cgcgatggcc
     9241 gagacgagcg ttccgccggt ggagtagccg gccgcgctgg ccgttgactg catgcagttg
     9301 ttctcgagaa tcgtcatgcg agtgcgggcc aacccaaggc ggtagagggt ggtccagatg
     9361 gcgtacgaga ggatgcaggc agtgatggcg acgccgatgc cccagccggt cttcaggccg
     9421 atgtacaggt tggtcagcga caggattccg cccagtacgg aacccataag aacggcccgc
     9481 caggtgagct gggccatcga gtcgccgcgg tttttataga cctgctcgta ccactggcgt
     9541 tcgatttcct ctggagagcc agtgaagccg ctcaggggga gcggtttttc gagttgatca
     9601 ctttgggagc gctcacgccc agagtcctgc gggggcgctg cggcgggcga ggcggtttcg
     9661 ttcttcatac gcgattcaac ctggcgcttg tgtcgaagcg gcgatctcgt ggctccgctg
     9721 cgcgtgtggg gggcccggtt ctcaaaccgg tctggcccga ggcggcgcgc tagtggctgg
     9781 gcgggattgt gggccactga ggagcgggtg gcaaggcagg gcggttgaga ttgcaggtgt
     9841 gaaagccgaa ccgataaatt ctgacttatc cgtagggcac actggacttt gtccggagac
     9901 ccgcatttga ttgtttgtaa cgcttcgctt cgcagcaggc gcgttggctg tagaagcgag
     9961 tcggttccgc cacgctggag atagccggac gttaagaatt ccgtccccgt tggtctgaaa
    10021 agagagcgga ggggaaagaa gtagtcctga gccggttaac gtgcggtcag cacgtagccg
    10081 gctcgggctg ttatatgggg tacgtacggg cgcgattgtg ctcggatttg ggtatcggcc
    10141 gcgggcgaga accactttgg cgtgcccggg cttagcgcct gggcatgccc gcgcttacag
    10201 cttgggcatg gcaccgggcg attacgtgat aaggtgatag agtgataagg tgataaaggg
    10261 cgccccgggg aatccgctta atcgagtttg ttgtggggcc gttcgcgagg gcatgcccgc
    10321 gcttacagct tgggcacggc acccgcggct gggcatggca cccgcgcttc agctcttcgt
    10381 ctgtggtatc cggcggaccg cgagttgata catcgtccgt ccctcgcgca agtactttat
    10441 ctcgaagttc gtcgcgatgc gtgcttccgc gacgccgaaa tccggatcgt cgaaggggat
    10501 gggctccagc tccggatggt tcagcaagag ttcgcgaatt acctcaaagt actcggcgtg
    10561 atcggtctgc acggcccacc ggccgccggg gatcaggcag gcggctgccg cgtcgacgaa
    10621 cggtttttgg aagaggcggc gcttgtggtg gcgacgtttg ggccaggggt cggggtggta
    10681 aacgtgcaac gccagcagcg agtcgcgcgg gcatttggcg cggacgaagt aactagcatc
    10741 ggcccgcagg atgcggacat tccgcaggtt ccaacgttgc atgcggtcga ccgcgaaacg
    10801 ataaaactcg ttggaccatt cgatgccgag aaaattgcgc tgcggatagg cttgtgcccg
    10861 ccgcaaaagg aacccgccct tgccgatgcc gatttcaagc tccaccgggt ggtcattgcc
    10921 gaagaaggcc gaccagcgca gattctcgag ttcgtccagg tggacgggta tgggcggcgc
    10981 cggtgcaggc ggtggtagcg gttgttcgga catagggggg ccattttcgg ccggcgcggc
    11041 aagcggagca aggaaaccgg ttgcggcggc cggcgtgtta tcctgccagg gcggcgcaga
    11101 tttcaattgc ttttcgttgt tcgcgtgtgc tattctgaag cggtgctggc ggggaggccg
    11161 gaggtggaat tcgggtgcgc ccgccggcag gtagtggatt gaaggcgcta ataggtttaa
    11221 gtgtgaattt gagggcgagg agagccgtgt atgaatacgt ggcgagccag gcatagccat
    11281 gttggccggt ggatagcgag tgttttattg ctcgtggcgg cgagtggagc gctggcgcag
    11341 gataggaatt atttgatcgt gtccgcgccg gactacgtgg gtagcgctcc cctgacgcaa
    11401 ttcatcaacc accggacgtc gagaggtttc aatgtatccg tctacagcgt accccccggc
    11461 acgagccggg aggacatcaa ggcccatatt caaagcctct ggggtacgcc ggacgcgccg
    11521 gagtttttgc tgatcgtggg cgacacgagc gggaccagct cgaccggcac cactattcca
    11581 cactgggtgg gacagggcag ccggcatgcc acgaccgacc taccctacgc gtgcatggac
    11641 agcggggacg actggtatcc ggatatgttc catggccggt tctcggtaac cagcgtcaac
    11701 atgctgcagc aagtagtgga caagacgatc tgcgtcgagt caggcaactt cagtgatccc
    11761 aattacgttc gccgggccgc aatgctggcc actgatgacc cgacggcggg ggcggcgacc
    11821 ctgcacgaaa cgatcatcag cagcttcctg gagccggccg gcttcaccgt cacgcgcgtg
    11881 tatgccgcgg ccggtggggg cacgtcgcac atttccgcgg cggtgaacgc gggcgtgctc
    11941 tttaccgtgt actttggtca cagcggctcg ggcggctggt cgtcgcccag tttcagccag
    12001 tccaacgtga atgccttgac caatgagggg ctatacgggc tgacaatggg ttggtcgtgc
    12061 aacaccgccc actatgacta tgacgagtgc ttcggcgaga cctggctgcg ggcggcgaat
    12121 aagggggctg cggcgtacct gtcagcttcg aactatgttt ggtggagcag ctccgcggac
    12181 tgggagtcct cgcggcggat ggagaactac tttttcaagg cggttttccg gaaggaaatc
    12241 tgcgaggtgg ggccggcttg gcaggatgca ctatggacga tcttaggcga tccagctttc
    12301 ggcccaacac acgaccacac acgcaacatc ttcgagcaga tggtcctcct cggcgatccg
    12361 gccttgagac tgccgctgcg ggcgctagac ctgtcgctgc cgaacggctg gcctgaattc
    12421 atcccgccgg acgtgcccac gccgatcgta ctgcgcattg ccgatgcggc ccaaagctat
    12481 ctgccggatt ccggccggct ttattaccgc tattccggcg acgagtatca gagtgtgccg
    12541 ctggttccgc tgggtgacgg cctctatgag ggcattctgc cgccaccggg gtgcaatgcg
    12601 aatccggaat actacttcag cgcgacgggc gaccaggagg acacggttta cctgccggag
    12661 ctagcgccac agcaggtgct cacttcaatc gttgcgacga caaccacgct gttcacggac
    12721 gatttcgagg ccgatcaggg ctggaccgtc tggaccgatc cggggctgac gaccggcgcc
    12781 tgggagcggg gcataccgct cgggacaggg caggtggggg caccggtggc tgactacgac
    12841 ggttcggggc ggtgctttgt taccgacaat cgcggcggcg cgtatgacgt ggacggcggg
    12901 ccgacgcagc taatctcgcc aacgatcgac atcagcggga tgagcgatgt gtacgtgcgc
    12961 tacgcgcgct gggtctattg cgatgatgag cttccgccgg cacaggactt tctagacatc
    13021 gagttctccg cggacggcgg ggcgaactgg acggcggtgg agcacgtttc ggggccgggc
    13081 ggctgggtca gacaggagat tcgcgtaagt gacttcgtgc cgctgacttc gcaattcaag
    13141 atgcggttct cgattaacga cacgccgaac aactcgctca ccgaagcggg cctggacgcg
    13201 gttgtgattt acgaccgtgc ttgcgggctg gaagcgatgc tcggcgatct aaactgcgac
    13261 ggcgtgctga acgtgtttga cattgatccg ttcgtgctgg ccttgacaga acccgcggga
    13321 tacgcggcgg ctcatccgga ctgcgatgcg aaccgggccg actgcaatca agacggcgaa
    13381 gtaaacgtct tcgacattga tcctttcgtg caattgatga caggaggctg atcatagccc
    13441 caagctgcag cgcgagcggc ctgagttggc cggcatgcag cggcgcgccg ctcaaagtcc
    13501 acggcgcgtg accagatatg tcgcgaagct gcctagccag tggccgccct cgtattgctg
    13561 gtcggccagg gcggccagtc cggcttgccg atgcgcgcgg gcggctgctt ggagagtcgc
    13621 gatgcgcggg tcgcccgccg gcaggctgga ggcgatgcct tccagcatcc aggcgcgcga
    13681 gaggttgagt ccgtccaggt gcacgagctg cgggtcggtg cggtcgctta cgacagccgg
    13741 cggcagccag tcgcgcgatt cgtcaccttt caggtccggt aagaacgcgc tgagccaagc
    13801 cgcaaattcc gccggcggaa ggatgcgccg catcacgtcg gcctcggcca gaatgggtga
    13861 gaggaaggct tggccgtcgg gttcgaaggc caggttcgcg ccgcggtcgt tctggaaata
    13921 ggccaaggca cggctggtaa ccagggcggc gaactcgcga ttgccgcgcg accgcgccca
    13981 gtcgagcagc aagccgagtg cgaaagcggt ctgcgtatgt tcgccggttc gcaccgggtg
    14041 ggtgagcttc ggcagccact ccgaaaagcg ttcgacagcc acttgctcga gcggttcgag
    14101 tgtgcgggcc cactcgcgcg cctgcggatc gtgccattcg tggagctcgg ccgagagttg
    14161 cagcagccac gccaacccgt acggacgctc gaagctcttg cgaccggggc cgcgaaggta
    14221 attcagctca tcagccactt tttctgggag gaagctctcg gccagggcgg ctcgggcgcg
    14281 aggcacgaag tccgcctgtg ggtacatgcg gcagagccgg accaggagcc agtgtccgtg
    14341 cacagaggag tgccaatcat aacatccgca gaatactgga gttagctcgc ggggcggggc
    14401 gacgtcggcg tcggagttga ggacatgtgc gatcttgtag ggatagtctt tatggacaca
    14461 tgcgagtgcc aggtttgcga atcgggctac ggcgtccgcg ctcagggtcg gctcaggctg
    14521 gggcggggag gtaagcagca ggccgatcgc gaagaagggg gataggggca tcgtttgtct
    14581 ccacgtgggt cttaatatac gcgggtctta atatactaga atggaactcg ggagtcaggg
    14641 ggcacaaacc acccgtggga tttttagatt gctttttgcg tgccgcgtgt gttattttac
    14701 ggtgacgccg cggacggcag ggggcacgtt ctcgtgtgcg ccggctgacc gcgtcggagg
    14761 tgaaggcaca ggtaatcgtg taacttcaac aaacggggtg aggagatccg gaatgaaggc
    14821 ttcgcgagcc aactgtagtt atagaggctg ggtggcggcg gccttgacgc tcgcgctggc
    14881 ggccagcgct gtggcggaca agaactactt aatcgtgtcc gcgtcgagtt acgtgggcag
    14941 tgcgcccctg acgcagttca tcaatcaccg caccgcccga ggctttaacg taatggttta
    15001 cagcgtcccg gccggaacga gccgcgatag catcaagggg tatattcaga gtctgtgggg
    15061 aacagcgaat caacccgagt atttgctgat cgtaggcgac accagcggca gcacctcgac
    15121 cgccaccacc atcccgcact gggtcggtgg cggcagccgg caagccacaa ccgatctgcc
    15181 gtatgcctgc atggatggcg gcggcgattg gtacccggac atgtacctcg ggcgattctc
    15241 ggtcaccagc gtggccatgc tgcagaacgt ggtggacaaa acaatctgcg tcgaatccgg
    15301 cgggttcgcc gatccggact atgcgcggcg cgcggtaatg ttggcaacgg atgattcgac
    15361 cgccgcagcc ccggccctgc atgattcgat catcagccaa tacctggagc cggccggctt
    15421 caccgccacg cgcgtctacg ccagctcggg cggcggttcg tctcatattt cggccgcggt
    15481 gaacgcgggc gtgctgttta cggtttattt tgggcacagc ggctcgaccg gctggtcctc
    15541 gccgggcttc agccagtcga acgtgaacgc gctgacgaac gacgggaaat acggattgac
    15601 gatgggctgg tcgtgcaaca cggcccacta cgattacgac gagtgtttcg gcgagacgtg
    15661 gctgcgggcc gcgcacaagg gctcagcggc ctacttgtcg gcctcgaatt acgtgtggtg
    15721 gggttcgtcg ggctcctggg agtcgtcgcg gcgcatggaa cgctacttct tccagtcgtt
    15781 ctttgtggac aacatctggg aggtcggccc ggcctggcag gcggcgctat ggaagattct
    15841 ggctgatccg gacttcggcc cggcccatga ccacacccgc aatattttcg aggagatggt
    15901 gttgctgggt gatccggccc tgcggctgcc gttccgggcc ctggacctgt tgttgccgga
    15961 cgggacgcca gagttcatcg cgccggatgt cccgacgacg atcagggtgc gcatcaatga
    16021 tgcaagtgag ctgtacgtac ccggttcggg gatgttgcac taccggtacg acaaaggtga
    16081 ctggctgacg gtcccgctgg agcctctggg cggctctctt taccagggca cgctgcccgc
    16141 tccgggctgc gatgctactc cggaatatta cttctgcgct accggggaca ggggcggaac
    16201 agcttgcttg ccggacaacg cgccggtgca cgttttgtct tcatccgttg cagtgattac
    16261 caccctgttg gccgaggatt tcgaaagcgc cgatggctgg acggtcgaaa ccgatcccag
    16321 cctgacgacc ggcgcctggg accgcggcat tccgcctgga gccggccaaa cgggagcacc
    16381 gcctgcagac tacgacggct caggcaggtg ctttgtaacg gataaccgcg tcggaaacta
    16441 tgacgtggac ggcggcccga cgcacctgat ctcgccaatg gtagacatca gccgcatgca
    16501 ggaggtacac gtgcgcttcg cggcctggat gcactgcgac gatgcgttgc cgccggaccg
    16561 ggattatctg gacgtggaat tctccagcga caacggccag acctgggtgt tggcggacca
    16621 catcccgtct cgtgacaagt gggtcgagcg cgatatcgtc gttaacaact tcgtcccgtt
    16681 gaccacgcaa ttcaggatgc gcttcacggc taacgatacg cccaacaact cagtgactga
    16741 ggcggcggtg gatagcattt ggctttacga taacgcctgt gggcccggac tgatgccggg
    16801 cgacatgaat tgcgacggca tcgtgaacgc cttcgatatc gacccattcg tgctggcgat
    16861 aaccgatgcg gacgcttatg cggccgtcca cccggactgc aacatttacg cgggcgacgt
    16921 taacgaggat ggctcgatca acgcgtttga cattgatccg ttcgtgcttt tgctgacgga
    16981 atagtagttg gactgagtga cgtttcctta actccgagaa gcggccggga tattgttccc
    17041 ggccgcttcg tttcgaaggg ggtgcaaagg ggtttcgtgc gacgcatgcc ttcacgcggc
    17101 aggcgccagc gagccgtttc tcggtatcga acaaacccaa ccactcggtt accgaagccg
    17161 gccttgaagt cctgacggct gtgttcgcaa aggggcagta cctcggtata ctgcgctaaa
    17221 tccgcccgaa tcggcgcggc ggatgcagag tgcgccctcg atgcaccgtg gctggttgcc
    17281 gaaaacgtca cctgcgttga gaacgcaccc tgcggataac gggtgcgtcg cggacgcacc
    17341 ccgcggacag ctacgagcag ggaaaccatg actgagattg cgaattctgc ggctcgcatt
    17401 gcggccgccg ggcggcgcgg gactgatgtg cgctcggatt gctgggttca gattacgctg
    17461 gccgaaagcg gcgggatcga tctgcagttg aaaaccaagg tcgaggtcat gtacggccag
    17521 gcgacgcgca agctggtgca cgaggcgctg gccgcactcg gcgtcacgaa cgcccgcgtt
    17581 gaaatcgagg acgcgggggc gctgccgtgg gtcatgctgg cgcggattga ggcggccgtg
    17641 cgccggctcg acgggcgcgg cgtgacggac atcatggcgg tgccggaatg cctgccggag
    17701 tttgtggccg gggcgcaata tccgaccgag cgcgagcgct tccggcgatc gcggctgtac
    17761 ttgccgggta acgagccgaa gttcatgctg aacgccgggc tgcaccagcc ggacggcgtg
    17821 attcttgacc tggaggacag cgttgcgccc gcggtgaagg acgaagctcg gctgatcgtg
    17881 cggaatgccc tgcgcagcat tgatttccgc ggctcggagc gcatggtgcg catcaaccag
    17941 ggcgagcgcg gcctggtcga tctggatttc ctcgtgccgc ataacctgca cgtggtgctc
    18001 attccgaagg tggaaagtcc ggagcaggtg cgcgccgtgg atgggcggat cgatcgcatc
    18061 ctcgcttcaa agcgccatgg cgacgccgcc ggtcgccggc cgttcctgat gccgatcgtt
    18121 gaaagtgcgc tgggggcgat taaggcgtac gaaatcgcga ctgcctcgcc gcacgtgatt
    18181 gcattgacga tcggcctgga ggactacacg gccgacatcg gcgcgcagcg gacgaacgaa
    18241 ggccgcgaga gtttctgggc gcggtcgcaa gtggtgaacg cggcgcgggc ggcgggcgtg
    18301 cagccgatcg acacggtgtt ctctgacgtg tcggacatgg agggcctgcg ggccagcgtg
    18361 ctggaggcga agggactggg attcgagggt aagggctgta tccatccgcg ccagatcagg
    18421 ccgattcacg aggctttcgc tccggcaccg gccgagcttg agaaggcgca aaagatcgtt
    18481 gcggcgttca aagaagccga agagaaaggc ctcggtgtgg tgtcgctcgg cagcaagatg
    18541 attgatccgc cggtggtgaa gcgggcgctg cggatagtca gattggcgga ggcgctcggg
    18601 tagggggtaa acttgacggg cgggaggtca agcgcgccgt agacaaagga gcgcgcgtgc
    18661 gttggtggcg tcggtactgg atggcagtcg cagcgctgct gggcttggcc gtctggggcc
    18721 tgacggacat tcgctggcgc gggcagttcc gccccgatgc gcccgagctg catcggacgg
    18781 atttcacggt ctacactaat gccgctgacg tactgctgag cgggggcgac ccgtatgccg
    18841 tcccggggcc gcgcgggtgg tactatctct atccgccgct ttttgcgctg ctggtgtcac
    18901 cgttgcggca tctggattcg cagtggcagg tcgtgatctg gttcgcgatt agcgtcggac
    18961 tggcgtatgg ctgtttgtcg gaagggcgcc gaattgggct ctggctttgg aagcgctgcg
    19021 cggagctgcc cagtgcgcag gtaatgcagc cgccggcgtg ggtgggcatt gtggcgtttg
    19081 tcactgtcct gctgccgctg ctcgaatgcc tgcaacgcgg tcaggtgggg gtactgattc
    19141 tgctctcctt aatgctcggc ctgagactga cgctgacacg cgagagtgca ttgcaggttg
    19201 cgggcggcgg ggtgcttttg gcgttgccgg ccgcaatcaa gataacgccc gcactgcctg
    19261 tactgctgta cgcagtcctc ctgggcacca ccggcccgcg ctggcgagga gctgcggcat
    19321 ggggcgggct ggttgcggga accgcgctgt tctgggtcgg cattcccggc gcggttgtgg
    19381 gctgggacga gaatttgcgc ttgttggccc gctggcgtga cacagtcgcc gccaacgaaa
    19441 tgcttgtcag cgaccgcggc attaatccac tgtcttggag caaccagagc ctcgaggtag
    19501 ctctgttccg cctcggggag attgcgcaac atgggtttcc gccgccactg cgagccgaac
    19561 aggtaggctc ggcaggtgtg cggctgacgc cctttccgcc acgaagcgcg gcggactggc
    19621 agaccccaat tagcgcgggg cgaatcgggc tgctggtatt gctgcttgcg gccgcggtac
    19681 gactttggcg cgctgcggat ttgtccggat tggcggcggc ctttgggctg ggctgcgcgg
    19741 cctcgctgct gatttcgccg atttcgtggg gacatcactt cgtcctgctg tttccggccg
    19801 ggttgctggt gccggcttgg tgcctgcttc gcggacggcg acggttggcg aagttgagcg
    19861 cgggaacgtt ggcagcgctg gtgctcatac actacatctg catgccagtg gcgggaccgt
    19921 ggagtgtgct ggggcttggg ttgacggtgt ggtttgtgat cgtgtgtgtt gcgttgatct
    19981 gggcggcgcg gcggggcggt gacacacact cgaccgacac cacacaattg gctagcactg
    20041 cttgaagctt gcgcttcatt cagtggcaca cgggcgaggt ggcgtcgggt taggcgaggc
    20101 gggcaagttg ccggattgtg gcccgcatgt ccttgggcag gggggcttcg cagctaatgg
    20161 cggcgccgct gatcgggtgc tcgaacgaga tcctcgcggc gtgcaatgtc aaacgttcga
    20221 tcagcggccg ttcctgaccg ctccgattca cgcggtaatc cggcttgaat tccgagagca
    20281 gaatcgactc gcgcccgccg taggtcggat cgacgccaag cgggtggccg atcgctgcca
    20341 ggtgagcgcg tatttggtgc atgcggccgg tgatcggtcg gcactccagc actgtgtggc
    20401 cggccaggcg ctcgacgatg tgatagcgcg taagcgaagg cttgccttta ctgtggttga
    20461 cctcggcccg ctgcatgcgc gagttgaaat acagcggcag atcgatttcg ccgtcctccg
    20521 cgacgtagcc gctgaccagc gcgagataga ctttctcgat gcggcgctcc atgaactgtt
    20581 gcaccaggcc gcgctgggcc gcaagcgtgc gggcgaacac cagcactccg ctagccccgc
    20641 gatccaggcg gtgcactacc cgcaggccgc cgtgaccgga taattcgggc tgccggctca
    20701 gtagttcgat cgcgctgggc ggcgtatttc gcccgggcac ggaaggcatg ccggccggct
//
LOCUS       DAOSYS010000034        20626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2120_length_20626_cov_5.497934, whole genome shotgun sequence.
ACCESSION   DAOSYS010000034 DAOSYS010000000
VERSION     DAOSYS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20626
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_20626_cov_5.497934"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PLU99_03530"
     CDS             complement(<1..1052)
                     /locus_tag="PLU99_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOO16159.1"
                     /translation="MSDALESFVRKTCAELGHDRTRMMDVVRAVQTQFGCVSNAAMNL
                     IARELKTHRVEVESVVSFYAFFSQRQKGKVIIRVCDDVIDRMAGAELVAKAFSEELGI
                     GFGQTSADGKFSLEWTPCIGMCDQAPAALINDVVVTELTGDRAREIVRELRNHMDPRK
                     LVRRYGDGNNGHELVNAMVNNNIRKRGPVIFSPTNRGEAIRKALAMSPAEVIRAVKTA
                     RLRGRGGAGFPCGMKWEFTRAAPGDRKFVLCNADEGEPGTFKDRVILTELPDRVFAGM
                     TIAGYAIGAQTGILYLRGEYAYLRAFLEDTLAKRRAEGLLGKNLCGKPGFNFDIRIQM
                     GAGAYVCGEETALISS"
     gene            complement(1130..1795)
                     /gene="hypB"
                     /locus_tag="PLU99_03535"
     CDS             complement(1130..1795)
                     /gene="hypB"
                     /locus_tag="PLU99_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOO16160.1"
                     /translation="MKKINVVESVLKANDAVARENRRRLDSAGLIALNLMSAPGSGKT
                     TLLEKTIAALGSDAVAVLVGDLQTTRDAERLGRMASQVTQINTGKGCHLTAPEVAAGL
                     EGLDLTGLRYIFIENVGNMVCPAAFDLGEHRRVALLSVPEGDDKVAKYPTLFQAADAI
                     LLTKVDLIGVLDYNRARVCEDLGRINTRAPLLEISAKTGQGMDLWLAWLREQQASATR
                     VGL"
     gene            complement(1782..2150)
                     /gene="hypA"
                     /locus_tag="PLU99_03540"
     CDS             complement(1782..2150)
                     /gene="hypA"
                     /locus_tag="PLU99_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOO16161.1"
                     /translation="MHEMAIAASLVEHVLEVAQQNGAARVSAIELEIGALQLVVPEAL
                     ELAFQAVTQGTLAEGATLKVTEIKAVAECRLCSTRFEAKIDDYLCPQCRQADVRLVAG
                     NDILLKSITCEVQQSGHEKD"
     gene            complement(2345..3034)
                     /locus_tag="PLU99_03545"
     CDS             complement(2345..3034)
                     /locus_tag="PLU99_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16162.1"
                     /translation="MEFRNSTDLDSERLLALFLRYSEPYRHDRLRVSVRYSRGADFSG
                     TCFYRDARIFVNLGRHVRYPYLLGTQIARAKSNRTHWWREIYRLSIADPYQLALFVYL
                     HELYHYLVKTAGRSPRRKESRCDRFAARVLVDDYGARVTDSRGRPVPREAWDFQEVEA
                     FVAKARRATATPPKPARGRKPKSQPKPSGPRMAATTASPEPATLPPLQPRAIPVVIYP
                     MTGQQGLLFDL"
     gene            complement(3127..3822)
                     /locus_tag="PLU99_03550"
     CDS             complement(3127..3822)
                     /locus_tag="PLU99_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16163.1"
                     /translation="MCKCAFNSTAGHLAVSVGVLAVLAGGARAGLLPITIVEQSHHAW
                     GHIEDVNYDFLGVVPVSGGCSKTIETDFIQIESNTLGSGADELNVYARAQSIEDYFYD
                     HSVQAEVHYLFHPNAIALTIAFDGEAGYHSFESGLVYLLRDVTTDETLDTRSWEFEDS
                     VGWVDDVLPYTNTYVMDLSHTYSLSVGAYAQLGDQREGFAQLAALLTPEPGSALLWGV
                     ALVAAARLRRRRA"
     gene            4215..4612
                     /locus_tag="PLU99_03555"
                     /pseudo
     CDS             4215..4612
                     /locus_tag="PLU99_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            5149..5221
                     /locus_tag="PLU99_03560"
     tRNA            5149..5221
                     /locus_tag="PLU99_03560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5182..5184,aa:Lys,seq:ttt)
     gene            complement(5368..5784)
                     /gene="rnk"
                     /locus_tag="PLU99_03565"
     CDS             complement(5368..5784)
                     /gene="rnk"
                     /locus_tag="PLU99_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOO16164.1"
                     /translation="MAARNVYITQHDAERLQEWLQLTARNNEKDRANLLALKRELDRA
                     RLIDSHKVPPDVVTMRSRVRLVDLRTEQASVVTLSFPDDAMQMDGRVCVLAPLGAALL
                     GCRAGQTVRFQVPGGTRTVRVEQILYQPEAAGHYHV"
     gene            complement(5875..6282)
                     /locus_tag="PLU99_03570"
     CDS             complement(5875..6282)
                     /locus_tag="PLU99_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16165.1"
                     /translation="MQVEIVSPKLSVRTPLRHLIDTRLRYALGPFRRRITAVTVTFER
                     LAGARGGRGLRCRVRVLPLRGSEVVVEATADSEAAAISCAAERAARCLRERTRRRWLT
                     GRRMHRTPRPEAPAAPGPAFCLPAAGPPLAIAE"
     gene            6572..7603
                     /gene="nhaR"
                     /locus_tag="PLU99_03575"
     CDS             6572..7603
                     /gene="nhaR"
                     /locus_tag="PLU99_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HOO16166.1"
                     /translation="MDWLNYHHLYYFWIVAREGSIAAASRRLLLSPPTISAQIRELEA
                     ALGAKLFKRVGRSLALNELGRLAARYADEIFALGQEFTDIAKGRTTETSLRFRLGVDD
                     VLPKTIVSRLLEPVLRQPQAVHVVCVEGTQAQLLPALAVHDLDLVLSDAPPDPTIKVR
                     AFGQLIGESGLTLCAAPKLARQLQPGFPKSLNDAPALLPTANSWGRRAMEQWFQSVDV
                     RPRRVAEFEDAALLQACGLRGLGFFALPDLVAREFASERIAKVVGHMGEARVRFYAIS
                     VERRLRHPAAVVLVETAAHVLPGDGVRPRVRQTARNSDKVARRRAAPGNGPKNTSRRR
                     SPRAGMSHG"
     gene            7596..9326
                     /locus_tag="PLU99_03580"
     CDS             7596..9326
                     /locus_tag="PLU99_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16167.1"
                     /translation="MVNRKHIRPGAERCRRGKPGEPLIAAKGGADDLVTLCRSILHMA
                     QRPTSRSEFLRELSTGVLQFADCDALHMRLVGIGGWSCTARIWPREEFVFESGDVDRP
                     QGDGLSLEALVTEYVRNVLGCAPDQLADHFTAYGSFWMEHSDAEDRCAEGLKSLAVIP
                     FEVSAGYAGLLRLDSRKAGVFAPDTIDFFEALAQIVGMGLADRRAHAALRERVKELTC
                     LYNISRITSQTEVPVAEQLRRIAELLPAAWQYPELAVAGIVLGKDTYATGDLVSASVR
                     QCQDIVLDGVKQGRVEIGYVADRPEFAQGAFLEEEADLLAAAAREIAEIIGRHRDAQH
                     GKELEEQIRRAGRLATIGQLVTGVAHEINEPLNAILGCAQLARKAPQLDGDVAGDLDD
                     IIAAALHARDIVNKLMLFARQRPPRKALLNLNDVVEQSLSILKTRCEKGGIELGQTLS
                     DELPLIFADSSQLQQVVVNLVVNAVQAMPGGGRLSIVTHGDADSATLVVQDTGVGMSQ
                     EVLAQCFEPFYTTKDVGEGTGLGLAIVHGIVTAHHGTIGCASQPGAGTRFEIRLPAAS
                     QIEDQEEDAR"
     gene            9316..10668
                     /locus_tag="PLU99_03585"
     CDS             9316..10668
                     /locus_tag="PLU99_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO16168.1"
                     /translation="MPANRPETMLVVDDAPVALRVIKRNLLSHGYQVLTATSVADAVA
                     ILEATPVDLVITDLKMPKVGGLDLVRHVHANFEDTAVIMITGYASIETAVRALREGAE
                     NYLPKPFTDEELLTAVRGALDRLHARRAANAERPPSARPALGLIGESQAMRRVIASIH
                     KAAATNATVLITGESGTGKELVARAIHYSGPRASAAFVAVNCAGIPDGLVESELFGHV
                     KGAFTGAVATRPGFFITANLGTVFLDEISELTLSAQAKLLRVLEDNEVQMVGASRPQA
                     VDVRVIAATNKDLQAQVSRQDFRDDLFFRLNILTIDLPPLRERGDDILLLIQHFNTKY
                     TKQAGRQPLEFSDAVLDVLRGYHWPGNVRELQNLIQRLAVMSDRERVSLAQLPSLMRF
                     SASGRGQQPRTLAEAEASHIGNVLASVNNNKTRAAEILAISRKALREKLKRYGLEPPT
                     "
     gene            11251..12477
                     /locus_tag="PLU99_03590"
     CDS             11251..12477
                     /locus_tag="PLU99_03590"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013996763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOO16169.1"
                     /translation="MQQFDKAADLMHLDPDVRAILGTTQDEVVVHFPVRMDDGRLEII
                     TGYRVHHNCALGPFKGGLRYHPRVNLDEVRALASWMTWKSALAEIPFGGAKGGLQIDP
                     TKYSITELERITRRFTFALGDNIGPEYDIPAPDVNTNAKTMAWILDTYLSTMPAHERQ
                     RQIHVVTGKPIAAGGSLGREKATGQGAVFTIQCWAEDQKLPLEKVKFILQGYGNVGSW
                     AARLLAQLGATLIAVEDVTGAIADERGINTEALAHHVRETGGVKGYPGKVIDHETFLS
                     TPADILIPAALESQITGDTAHLLKVRLVAEAANGPTTPDGDAILQSRGIDVIPDFLCN
                     AGGVVVSYFEWLQNKRSESWELSEVDCKLRKKMTSAYTRVRDKARELKVDWRTAAYVL
                     ALAKLEAVYKERGIFP"
     gene            12704..13126
                     /locus_tag="PLU99_03595"
     CDS             12704..13126
                     /locus_tag="PLU99_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16170.1"
                     /translation="MKRRNLGDLREWGRVSEELAAIQRQGGLDEYQQELVHMLRFRDN
                     WRLREMALTSIKRVEAVSEELAREMLKIMNDDELYDEVRMLAAEALADALARARAANR
                     NALAGVVRDAIDTMHAILDGPQPPVLQETVRRALAALE"
     gene            13176..14339
                     /locus_tag="PLU99_03600"
     CDS             13176..14339
                     /locus_tag="PLU99_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO16171.1"
                     /translation="MIIGIPREIMPGERRVAALPETVREYVNMGFQVLVEASAGRGVF
                     HSDQDYRAAGAEIVPDASTLFARADLVLKVKQPCFNETVGRHEAELIREGALLITFLH
                     PAAPASHSVVRTLCERNITALTMDGVPRISRAQTMDALTSMSTITGYKSVLIAASHFP
                     RFMPMIGTAIGATKPANVLVVGTGVVGLQAIATAKRLGATVTAVDIRPDARREAGSLK
                     GTKVAGFEVPDEVALAPGGYARALPTEWLVRERALLDTLLPQTDIAILSALVPGEVAP
                     ILITEEAVRTMRPGSVIVDVAIDQGGNCALTQFGREVVVQDVYICGTANIPGSVAVDA
                     SWLYSNNMLRYVQNLFKDGPQRPCFEDEIVKQSLVTRDRKIVHAGTLKAMRLG"
     gene            14359..14694
                     /locus_tag="PLU99_03605"
     CDS             14359..14694
                     /locus_tag="PLU99_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO16172.1"
                     /translation="MNEVLLMLGIFVAVFGLGYLFITRVPQMLHTPLMSMTNAISAVT
                     ILGALLLFSVETSAFEKVCGAAAIITAIFNVAGGFVITDRMLGMFRRKNVAPLKFSNT
                     GDNSPSGQD"
     gene            14698..16692
                     /locus_tag="PLU99_03610"
     CDS             14698..16692
                     /locus_tag="PLU99_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOO16173.1"
                     /translation="MVSGLLVDLLVIALLLAGVWQFRTPPGARRGNWTAAVALVVATV
                     VVLWRHGLESVPLVVIVGLLGIGLGVWVALRATMLQIPALVAFQHGMGGAAALLVSFV
                     ELTRDATKAAITPPEVAALLGLIVGAGTFSGSLIAAGKLDRRLPQTPVSLPRHDAMLL
                     CLALLVVAAGIVAALAKDIALLSTVLVVVVALSIAIGALFAIRIGGADMPVLISFLNA
                     TAGVAAALCGVAIGNRLLIACGATVAASGSILTHVMCRGMNRGLTSIFAGTRSSAPPA
                     GAAPAGASHSRPEPAAPAPVSVKTSAAPTDVSSNGDPYNHAATLLREARRIIIIPGYG
                     MALAEAQEGVVKLAEQLLEQGKSVKFAVHPVAGRMPGHMHVLLAEADVDYSLLFELKD
                     VNHEFAETDVALVVGACDVVNPAAIERPDTPISGMPILNAHEARHVIVCNLDERPGYS
                     GVENPLYKNPKTVLLFGDAQESVEKLREGLSPAPTDVSSNGDQYNRAATLLREARRII
                     IIPGYGMALAEAQESVVKLAERLLEQGKSVKFAVHPVAGRMPGHMHVLLAEADVDYSL
                     LFELKDVNHEFAETDVALVVGACDVVNPAAIERPDTPISGMPILNAHEARHVIVCNLD
                     ERPGYSGVENPLYNNPKTVLLFGDAQESVEKLCKGLSASA"
     gene            complement(16922..18238)
                     /locus_tag="PLU99_03615"
     CDS             complement(16922..18238)
                     /locus_tag="PLU99_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOO16174.1"
                     /translation="MMTVSSGLTLILLAQAVTGWALSRPPAAQPGDCVHLVRFQPGPP
                     APPDPDPPTVISSRRLLIRDPYAPNPSIQKMPSQWLGVRLTPIPEPLAAHIGENGVMV
                     ANVVQDSPADRAGVERYDVIVSLDSQQVADSEDLFAALEDIKPGQSVPLTVIRGAQKK
                     EMTITPAERPTAPQVTWKYDEPEDIIDRQMRLRGHKLMPGPGGAWQWEELGPLHTFPD
                     ILRELHLPDFDVSIDPFAEEDVDLDVKARVEVRVQVDDDGNTLTIWRDKDGRINVKRV
                     DPAGNESSATYEDMDEFRKADPDAYKAYRPFSSRGPESWNFVYPQREHLNKLRQDFQV
                     EIEATLRKALDRAKAAEEEAARAVERVRERLEQYRSNATDSSGSVDEQRTVIKKMQRL
                     SVQVEDGHVTVTVDDGQGPQTYEFQSKEEFKQQQPELYDRCKQLLE"
     gene            complement(18251..18802)
                     /locus_tag="PLU99_03620"
     CDS             complement(18251..18802)
                     /locus_tag="PLU99_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16175.1"
                     /translation="MNDGSPELERLISRYLDGEATPAERRDLESRANADAAIQSLLAE
                     TAAIDREVGRAMRMAMRRTLPLPARRVKKVHWIQAVGFAAAAACIGLVFFLSSPRPSN
                     GPKHSVQRPQPSAPMRASWFAPPERPVDVVDQVDPADVRPQVRWRDTDRHWIVVPARQ
                     NGQFLLIQMDRLRTRAIRIQDDF"
     gene            complement(18799..19401)
                     /locus_tag="PLU99_03625"
     CDS             complement(18799..19401)
                     /locus_tag="PLU99_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOO16176.1"
                     /translation="MSKPSDPPVTIAATVDGEALQKLVADVQRGDRPAADRLVREHDS
                     WLRSVVYGITGRPDWVDDVVQQVWIQVWEHLDSLKDPHRLRSWLYSVARHAAIDAGEA
                     RRRRDGRTGSLDVLPEAAAAHNASPVGAVLQDELQTTLLRAVQALPALYREPFVLRHL
                     ENWSYAEIGDCLGLPVETVETRLVRARRLLREMLYGKVDV"
     gene            19555..>20626
                     /locus_tag="PLU99_03630"
     CDS             19555..>20626
                     /locus_tag="PLU99_03630"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16177.1"
                     /translation="MPLPRVLKDLLSLPTGTFVEGAVLEYIESTCSNLKGVTYTSDRY
                     GNILAHYRNRPPGSTPLVFAAHTDHPGFVAQEMVDAKTVRAAFRGGVASSYMEKAKVR
                     FWSGGRWINAKVRELTRTVAAPRFGAGATRPEEGLLTVAQPVEPNSPGMWDLGGPVLD
                     GDLVRGIACDDLAGVAAMLALLARLSKKQAAAEAYCLFTRAEEVGFIGAIGAAEARTV
                     SKKLPIVAIETSKALPSAPIGAGPILRVGDRASIFTPELTAWCGQVAEKLAKRRKGFK
                     FQRKLMDGGTCESTAYVTYGYDATGLCLALGNYHNMNEKTGKLGSEYVSLSDWRSLVD
                     WFEALVLDPDGFAPTESKNMARV"
BASE COUNT         3709 a         6448 c         6698 g         3771 t
ORIGIN      
        1 caggagctga taagggcggt ttcctcgccg cacacgtaag cgccggcgcc catctggatt
       61 cggatatcaa agttgaagcc gggcttgccg cagaggttct tcccgagcag gccttcagcc
      121 cgccgcttgg ccagcgtgtc ctccaggaag gcccgcagat aggcgtactc cccgcggagg
      181 tacaggatgc cggtttgggc gccgatggcg tagccggcga tggtcatgcc ggcgaacacg
      241 cggtcgggca gctcagtcag aatgacgcgg tccttgaagg tgcccggctc gccttcgtcg
      301 gcgttgcaga gcacgaactt gcggtcaccg ggggccgccc gtgtgaattc ccatttcata
      361 ccgcagggga aaccggcgcc gccgcggccg cgcaggcgag cggttttgac cgcccggatc
      421 acttcggcgg gcgacatggc cagggccttg cgaatggcct ctccgcggtt ggtgggcgag
      481 aaaatgaccg gcccgcgttt gcgaatgttg ttgttcacca tcgcattgac gagttcgtgg
      541 ccgttgttgc cgtcgccgta gcgccgcacg agcttgcgcg gatccatgtg gttgcgcagc
      601 tcgcgcacga tctcgcgcgc ccggtcaccg gtcagttctg tcaccacgac gtcattgatc
      661 aacgccgccg gcgcctggtc gcacatgccg atgcacggcg tccattcgag cgagaacttc
      721 ccatcggcgc tggtctgccc gaagccgatg cccagctctt ctgagaaggc cttggccacc
      781 agctccgcgc cggccatgcg atcgattacg tcgtcgcaca cgcggatgat gaccttgcct
      841 ttctgccgct gggagaagaa agcgtagaag gagacgacgc tctccacttc cacgcgatgg
      901 gtcttcagct cccgcgcgat caggttcatt gccgcgttgc tgacgcagcc gaactgcgtc
      961 tgcaccgccc gcacgacgtc catcatgcgc gtccggtcgt gcccgagctc ggcacaggtt
     1021 ttccttacga agctctcgag tgcatcactc ataagctacc tcatttgcgg cgccggcagc
     1081 ccgccgggca gccttccgcc gtgagccgcg atgaatcgcg gcattcagcc tacaagccaa
     1141 ctctggtcgc gctggcttgc tgctcgcgca accacgccag ccacaagtcc atgccctgtc
     1201 cggttttcgc agaaatctcc agcaatggag cgcgtgtgtt gatccggccc agatcctcgc
     1261 acacccgcgc gcggttgtaa tccaaaacgc cgatcaagtc gactttggtc agcagaattg
     1321 cgtccgccgc ttggaacaag gtggggtact tcgccacctt gtcgtcgccc tccggcacgc
     1381 tcagtagcgc cacccgccga tgttcaccca ggtcgaaagc cgccgggcag accatgttgc
     1441 cgacattttc gatgaagatg tatcgcagcc cggttaagtc caggccttcc agccccgcgg
     1501 cgacctccgg ggcagtcagg tggcagccct tgccggtatt tatctgtgta acctgcgacg
     1561 ccatccgccc cagccgttcc gcgtcgcgcg tagtctgcag atcgccgacg agcacggcca
     1621 ccgcgtccga gccaagtgcg gcgatcgttt tttcaagtaa tgtcgtcttg ccgctgccgg
     1681 gtgcgctcat caaattcaga gcgattagac cggcgctatc gagccgccgc cggttctccc
     1741 gagcgaccgc atcgttagcc ttgagcacac tttcgacgac gttaatcttt ttcatgtcct
     1801 gactgctgga cctcgcacgt gatcgatttc agcaggatat cgtttcccgc cacgagccgc
     1861 acgtccgcct gccggcattg cggacagagg taatcgtcaa tcttcgcctc gaagcgagtc
     1921 gaacacaggc ggcattccgc gaccgccttg atctccgtga ccttcagcgt cgcgccctcg
     1981 gccagagttc cctgggtgac ggcctgaaag gctagttcca aagcctcggg cacgaccagt
     2041 tgcagagcgc cgatttccag ctctatagcc gaaacccggg ccgctccgtt ctgctgggcg
     2101 acctccagca cgtgctctac taggctggcc gcgatagcca tttcgtgcat tcgaagctcc
     2161 tggcgaactc tcgcggcagc atcggaacgg ctcccatagt tctgcaatcc acgggccaca
     2221 aagagacgcg tgcggcccga tttcggcccg cagagcaggg gcgctatccc gcgcggcgcg
     2281 ccggcgcgcg gattctcgcg caaaaactgg caatcatgcc ccgctgtccg attatagagg
     2341 cagcttacaa atcaaacagc agaccttgct gaccggtcat gggataaatc acgaccggaa
     2401 tggccctcgg ctggagcggc ggcagggtgg ccggttccgg ggaggctgtg gtggccgcca
     2461 tgcgagggcc gctcggcttg ggctggcttt tcggtttcct gccgcgggcc ggcttcgggg
     2521 gtgttgccgt cgcgcggcgg gctttggcga caaacgcctc cacctcctgg aagtcccaag
     2581 cctcccgtgg gaccggtcgg ccccggctgt ccgtgacccg cgcgccgtag tcgtccacca
     2641 ggacgcgcgc cgcaaaccgg tcacagcggg actccttccg tcgcgggctc cggccggccg
     2701 tcttcacgag gtagtggtaa agctcgtgca ggtaaacgaa cagcgcgagc tggtacgggt
     2761 cggcgatgct cagccgataa atctcgcgcc accagtgggt gcggttcgac ttggcccggg
     2821 cgatctgcgt gcccagcaag tacgggtaac gcacgtgccg gccgaggttt acgaaaattc
     2881 gggcgtcgcg atagaaacac gtgccggaga aatcggcgcc gcggctgtag cgcacgctga
     2941 cgcgcagccg gtcatggcga tagggctcgc tgtatcggag aaagagcgcc aggagtcgct
     3001 cgctatccag atctgtcgaa ttgcgaaact ccattccgcg cctcgctcct cggttttttc
     3061 atgagaacgc gaggcgtgaa aaattgcaaa tgcgcggcga gcggaccgga cgaagcggct
     3121 tctgcatcag gcgcggcggc gccggagacg cgctgctgcg acgagcgcca cgccccacag
     3181 cagggctgag cccggttccg gggtgaggag cgccgccaat tgtgcgaaac cctcgcgctg
     3241 atctcccaat tgggcgtagg cgccgaccga gaggctgtag gtatggctga ggtccatgac
     3301 gtaagtgttc gtatatggca gcacgtcgtc cacccagccc acggaatcct cgaattccca
     3361 ggatcttgta tccagggttt cgtccgtggt cacgtcccga agcaagtaaa ccagcccact
     3421 ttcaaacgag tggtagccgg cctcgccgtc gaaggcaatc gtcagcgcaa tggcatttgg
     3481 atggaacagg tagtgtacct cagcttgaac actatggtca tagaaatagt cctcaatact
     3541 ttgggcacgc gcgtagacgt tcagttcatc cgcgccactg cctaatgtat tgctctcaat
     3601 ttgaatgaaa tcagtctcga tagttttgga acaaccgccg ctaaccggca ctacgcccag
     3661 aaaatcgtaa ttaacatcct cgatgtgtcc ccaggcatgg tgcgattgct caacaatggt
     3721 gatcggcaat agccccgcac gcgcaccgcc cgcaagcact gccagcacac ctacactcac
     3781 cgccagatgg cccgccgtcg aattgaacgc acatttgcac attctttcgc tcccagttgc
     3841 gacgtcctgg caggcggccg gcacacacgc tgcatgattg gcggccgccc gtgctgtccg
     3901 gcttagtgca cgcgctccac gccggaagca ccctctctat gtgagtgtat ggcgcggcgc
     3961 gagaggataa ctcatcaaat atgtaacggt ccgaattggc ggcagttagc tctctgatac
     4021 cacgactgac gagtgcgact gtcgcgacag accggattga ctgggtctcg cccggcgtgt
     4081 ctgtgcaaag ccgggcaaga gggcttgctc aatagattcc gctcgcacgg cccggcacta
     4141 aacgctgtcg ccgccatgcc agggccggta aactcgcttc agacgcagcc gattgtatgt
     4201 tcagaggatc aaccatgttt caaagagcat tctcgcaagc cccctgggag tcccagatcg
     4261 gctactgccg cgccctgcgc gcgggccggc acatctacgt cacgggcacc gcgccggtgg
     4321 cagacgacgg cggcgttttc gccccgggcg acgcctacgc ccaggcccgc cgctgcatcg
     4381 aacttattgg gcgtgcgctg cgcgaactgg gggccgacct ggcacaggtc gtgcgcacca
     4441 gaatgttcgt gaccgaaatc gcccgctggc ccgaattcgg ccgcgcgcat caggaattct
     4501 tcggagcaca cccacccgcg acaccatggt cgaggttaaa tcgttgattc acccggatat
     4561 gctcatcgag attgaagccg aggccgtgtg cccggatgac cgcgacggct gaggggtgcc
     4621 gctgccgcac ccgccagaag cgggacggct gggttagccg tctctaaatt cgcggtgcac
     4681 gactccggca cggcgttcgc gggcgcgcac tgagtagcag aaccggctcg tgccgagccg
     4741 gagtctgacc agagtgcccc aatcgctcag aacgcacccc cagcacgcac cgcgggcctt
     4801 gcgtgcgctt caagctcgga tcctttgcct gtccgcgagc ggccccatcc gccgcggaaa
     4861 ccgcgactcc cccctcttga tcccttgatc cctagatccc ttgatccctc tcgatgttct
     4921 gtcccggcat tccggggcgg cacgccccgg cgctgctccc gggcgtcacg ctgcgcagct
     4981 acacccgcct tcagtcacgt gtcggcgaat ttcttgacgg agtgtaagct cgtgactagc
     5041 ttacgcatgt cggcccgcag ccgcggtgca ggctcggctt gagaacggaa accgaaactg
     5101 atctcgggcg ggggacggcg ctgccgtagc cgctgcacgt gcttttttga gggtgtagct
     5161 cagtcggtag agcaacggcc ttttaagccg tgggtccagg gttcgagtcc ctgcaccctc
     5221 attttcccac gagtcgtctg gaacgagggc ttacgcacgg ctcgacggca cgtggccgaa
     5281 tggctgcggg cgccgacggc tgcttttgcg cagagctcgt ccggcgcccg actacgggtc
     5341 aaacggaact accggtattg ccggaatcta cacgtggtaa tgcccggcag cctccggctg
     5401 gtagagaatc tgttcgacgc ggacggtgcg cgtgccgccg ggcacttgga aacgaacggt
     5461 ctgcccggcc cggcaaccga gtagcgccgc accaagaggc gccagaacac agacccgccc
     5521 atccatctgc atggcatcgt cggggaagct gagcgtcacc acgctcgcct gctccgtgcg
     5581 cagatccacc aggcgcactc gcgagcgcat ggtcacgaca tccggcggga ccttgtgcga
     5641 atcgatcagc cgcgctcgat cgagctcgcg cttcaaggcg agcaggttgg cccggtcctt
     5701 ttcgttattg cgtgccgtca actgcagcca ctcctgcagc cgttccgcgt catgttgggt
     5761 gatgtatacg tttcgcgccg ccatggctgc gctccttttc tcacacgtca agaaaattgc
     5821 ccgaaaggca ccttacgttt tagcaatcgc tcctgttgaa cgcgttgggg tccgtcactc
     5881 cgcgattgcc agcggcgggc cggccgcggg tagacagaac gcagggccgg gtgcagccgg
     5941 cgcttcgggg cgcggcgtgc gatgcatgcg gcgtccggta agccaccgcc gccgggtgcg
     6001 ttcgcgcagg cagcgcgccg cgcgttcggc cgcgcagcta atggccgccg cctcactatc
     6061 ggccgtggct tcaacgacca cttcgctgcc gcgcagcggg agcactcgca cccggcagcg
     6121 cagaccgcgc ccgccgcgcg cccccgccag gcgctcaaag gtaaccgtga ctgccgtgat
     6181 acggcgcctg aagggcccga gggcgtaccg caggcgcgtg tcgatcagat ggcgcagcgg
     6241 ggtgcgcaca ctcaacttgg gggacacgat ctcgacctgc atgccgcgtc tcctttccgt
     6301 cggcgcccac agccggcgcc ggcccacggt agtctcgcct aacgtattta aaccggccgc
     6361 gccttattaa caagatcaaa attatcacat tgagcgttag gttttaccta atagcattca
     6421 agcaaggctt ggccggcccg gcggggcgtc agttcgccgg ctcgaggcgt tgccaccagg
     6481 gccgcagggc ttgccggcgc cgcggcgcgc tagttcgtaa aatcagaaca attggcttga
     6541 cggtgcgata aatacgaata agatgcgggg aatggactgg ttgaactacc accacctcta
     6601 ctacttctgg attgtggcac gcgagggtag catcgcggcc gcctcgcgcc ggctgcttct
     6661 atcaccgccg accatttcgg cccaaattcg cgagctggag gcggcgctgg gcgcgaagct
     6721 attcaagcgc gtcgggcgca gcctggcgtt gaacgagctg gggcgcctgg cggcgcgcta
     6781 cgcggatgag atatttgcgc tgggccagga gttcacggat atcgccaaag gccgcacgac
     6841 cgagacgtcc ttgcgtttcc gcttgggggt cgacgacgta ttgccaaaga ccatcgtcag
     6901 ccgcttgctt gagccggtgt tgcggcagcc ccaggcggta catgtcgtgt gcgtggaagg
     6961 cacccaggca cagttgctgc cggccctggc agtgcacgac cttgatctgg ttctctcaga
     7021 cgcgccgccg gatccaacca tcaaagtgcg ggctttcgga cagctcatcg gcgagtcggg
     7081 cctgacgctc tgcgccgcgc cgaagctggc gcggcaacta cagccgggat ttcccaaatc
     7141 gctcaacgac gcccccgcac tgctgccgac cgcaaactcc tggggccgcc gcgcgatgga
     7201 gcagtggttt cagtcggtcg acgtacggcc gcggcgggtc gcggagttcg aggatgcggc
     7261 cttgctgcag gcctgcggct tgcgtggctt gggatttttc gcgctgccgg atctggtagc
     7321 gcgggaattt gccagcgaac gtatagccaa agtcgtggga catatgggcg aggcccgggt
     7381 gcggttctac gcgatcagcg tggagcgacg cttacggcac ccggctgccg tggtgcttgt
     7441 cgagacggcc gcgcacgtct tgccgggcga tggcgtgcgc ccgcgagtgc gccagacggc
     7501 gcgcaacagc gacaaggtgg caaggcggcg cgctgcgccc gggaatggcc cgaagaatac
     7561 cagccgccgg cgctcaccac gggccggaat gagccatggt taacagaaag cacatccggc
     7621 ccggtgcaga gcggtgcagg cgggggaagc cgggcgagcc gctgatcgca gccaagggcg
     7681 gtgcggatga tctggtaacg ctgtgccgga gcatcctgca catggcccag cgtcccactt
     7741 ccagaagcga atttttgcgc gagctgagca ccggcgtgct gcaattcgcg gattgtgatg
     7801 cgttgcacat gcggctggtg ggaatcggcg gctggagctg cacggcccgc atctggccgc
     7861 gcgaggagtt tgtttttgag tcgggcgatg tcgaccgacc gcaaggcgac ggattatcgc
     7921 tcgaagccct cgtgacggag tatgtccgca acgttctggg ctgcgcgccg gatcaactgg
     7981 cggaccactt cacggcctac ggcagtttct ggatggaaca ctccgacgcg gaggaccgat
     8041 gcgcagaagg cctgaagtcc ctcgccgtga tccctttcga ggtcagcgcg ggctacgcgg
     8101 gtttgctgcg gctcgacagc cgaaaggcgg gagtgtttgc cccggacacg atcgattttt
     8161 tcgaagccct ggcgcaaatc gtaggaatgg gactggctga ccggcgtgcg cacgcagcgc
     8221 tgcgcgaacg ggtgaaggag ttgacctgcc tgtacaacat ttcgcggatc acgagtcaga
     8281 ctgaagttcc ggttgccgaa cagttgcggc gaatagcgga gctcctgccg gccgcctggc
     8341 agtatcccga actggccgtg gccggcattg tgctgggaaa ggatacatat gccacgggtg
     8401 atctcgtatc ggcctctgtg cgccagtgcc aggacattgt tttggacggg gtgaagcagg
     8461 gcagggtcga aatcggctat gtcgctgatc gtccggaatt cgcacagggg gcgtttctgg
     8521 aagaggaggc ggacctgctc gctgccgcgg cccgggaaat tgccgagatt atcggccggc
     8581 atcgcgacgc gcagcacggc aaggagttgg aggagcagat tcgccgggcc ggccgcctcg
     8641 caacgatcgg tcagctcgtt accggcgtag cgcacgaaat caatgagccc ctgaatgcaa
     8701 tcctgggatg cgcgcaactg gcccgaaagg cgccccagct tgacggggac gtggccggcg
     8761 acctggacga cattatcgcc gccgcactgc acgcacgcga catcgttaac aaactcatgc
     8821 tcttcgcgcg tcagcgtccg ccgcggaagg cgttgctgaa cctgaacgac gtggtcgagc
     8881 agagcctgtc tattctgaag acgcgctgtg aaaagggggg catcgagttg ggccaaacac
     8941 tgtccgatga actgccgctg atcttcgccg actcgtcgca attgcagcag gttgtggtca
     9001 atctggtcgt aaacgcggtg caggcgatgc ccgggggcgg ccggctgagc atcgtcacac
     9061 atggcgatgc ggattccgcc acattggttg tgcaggacac cggcgtgggc atgagccagg
     9121 aggttctcgc ccagtgtttc gaacccttct acaccaccaa ggacgtcggg gaggggaccg
     9181 ggctgggcct ggcgatcgtc cacggcatcg tgaccgcgca ccacggaacg atcggttgcg
     9241 cgagccagcc cggggcgggt accaggtttg aaatccggct gccggctgct tctcagatcg
     9301 aggatcagga agaagatgcc cgctaaccgg ccagagacga tgctcgtggt tgatgatgcg
     9361 ccggtcgcgc tgcgcgtcat aaagcgaaat ctcctgtcac atggatatca ggtactcacc
     9421 gcgaccagtg tggctgacgc tgttgcgata cttgaggcaa cgccggtcga cctggtgatt
     9481 actgacttga agatgcccaa ggtcggcgga ctcgacctgg tccgccacgt gcacgccaat
     9541 ttcgaggaca cggcggtaat catgattacc ggctacgcct cgatcgaaac ggccgtgcgg
     9601 gcactgcgcg agggggcaga gaattatctt cccaaaccgt ttaccgacga ggagttgctg
     9661 acggcggtgc gcggcgcatt ggaccggctg cacgcgcgcc gggcggccaa tgcagagagg
     9721 ccaccctcgg cccgtccggc gctggggctg atcggcgagt cgcaggccat gcgcagggtc
     9781 atcgccagca tccacaaggc ggccgccacc aacgccacgg tgctgattac gggcgagagc
     9841 ggaaccggca aagaactcgt cgcccgagcc atccactaca gcggcccgcg ggcctcggcc
     9901 gccttcgtgg cggtgaattg cgcaggcatt cccgacggcc tggtggaaag tgaactgttc
     9961 ggccacgtga aaggggcctt cacgggtgcc gtcgccacgc ggccgggctt tttcatcacg
    10021 gccaacctgg gcacggtctt cctggacgag atcagcgagc taacgttatc ggcccaagcc
    10081 aaactgctcc gggttctgga agacaacgag gtgcagatgg tgggagccag ccgcccgcag
    10141 gcggttgacg tccgagtgat tgctgcgacg aacaaggacc tccaggccca ggtgagccgg
    10201 caggactttc gggatgatct gttctttcgc ctgaacattc tgacgattga tctgccgccg
    10261 ctgcgtgaac ggggcgatga tatcctgctc ctgattcagc acttcaacac gaagtacacc
    10321 aagcaggccg gaaggcagcc gctggagttt tcggacgcgg tgctggacgt tttgcgcggg
    10381 tatcactggc ccggcaacgt gcgggagttg cagaacctga tccagcgcct ggcggtcatg
    10441 agtgaccggg agcgcgtttc gctggcccaa ctgccctccc tgatgcgttt ttcggcctcc
    10501 ggccgcggtc aacagccgcg cactctggcc gaggccgagg cgagccacat cggcaatgtg
    10561 ctggccagcg tgaataacaa caagacgcgc gctgccgaaa tcctggcgat aagccgcaag
    10621 gcgctgcggg agaaactcaa gcgctacggg ctggagccgc caacctgagc ttggccaatc
    10681 agccttgggg ggtcgcctgg cacctttcta tccggcgcgg atcgaatata gccgcgccgc
    10741 gtgaacctgc cggccgggct ttggcgctcg cccggagccc gtggcaagtc gaaaaaccgc
    10801 gtttaacgcg tcagaacagc gattattgac cgcacggcgg acaacgcggc gggtgacggg
    10861 cggtgcgtcc gacggattaa gcgcggctgg tccggaactt gcattaggac agcattgtga
    10921 ggagtatcag tggacgcccc caaccgttaa acgagggccg gcgtccgccg gtcagccgcc
    10981 ggcggcaaac gcggcggcgg agattcggcg cccaaactgc tgggcctgcg aactgcgagc
    11041 aacgtcagac gtgtcgcctg ccgaaaaccg cgggcgcagc ttggcattgc gaggaataca
    11101 gctaggtccc agcgccccgt gtatggcggc gctggttcag tagtgttttc acaagattat
    11161 ggaggacatg ttgtgagtaa tcgctcatcg tcgaccgttc cgtcaggaac cggcgagaac
    11221 ggcgctcccg tgagcgtcta ccggaacgtc atgcagcagt ttgacaaagc tgccgatctg
    11281 atgcacctcg atccggacgt acgcgccatt ctgggaacta cgcaggatga agtggtcgtc
    11341 cacttcccgg ttcgcatgga cgacggaagg ctggaaatca tcactggcta tcgcgtgcat
    11401 cacaactgtg ccctcggccc cttcaagggc ggtctgcgat accatccgcg cgtaaacctc
    11461 gacgaagtcc gggccctggc ctcctggatg acgtggaaat cggccctggc cgaaattcct
    11521 ttcggcggcg ccaaaggcgg gctgcaaatc gacccgacca agtactcgat cactgagttg
    11581 gagcgcatca cacgacgctt cacctttgcg ctcggcgaca acattggccc cgaatacgac
    11641 atccccgccc cggacgtgaa caccaacgcc aagaccatgg cttggattct ggacacttac
    11701 ctgtccacca tgccggccca tgagcggcaa cgacagattc acgttgtaac cggcaaaccg
    11761 atcgcagccg gcggaagcct cggccgcgag aaggcgaccg ggcagggggc ggtctttacc
    11821 atccagtgtt gggcggaaga ccagaagctg ccgctggaaa aagtgaaatt cattcttcag
    11881 ggctacggca atgtgggttc gtgggcggcg cgcctgctgg ctcagttggg ggccactctg
    11941 attgcggtcg aagatgtcac gggcgccatc gccgacgagc gcggcatcaa caccgaagcg
    12001 ctggcccacc acgtgcgcga gaccggcgga gtcaaaggct atccaggaaa ggtgatcgat
    12061 catgagactt tcctgagcac gccggccgac atccttatcc cggcggccct ggaaagccag
    12121 atcaccggcg atacggcgca cctgctgaag gtccgcctgg tggctgaggc ggccaatggc
    12181 ccgacgaccc cggatggtga cgcgatcctg cagagccgtg gcattgacgt cattccggat
    12241 ttcctgtgca acgcgggcgg agtggtcgtc agttacttcg agtggctgca gaacaagcgc
    12301 agcgagtcct gggaactgag cgaagtggac tgcaagctgc gcaagaagat gaccagcgcc
    12361 tacacccgtg tgcgtgataa ggcccgcgaa ctcaaggttg actggcgcac ggcggcgtac
    12421 gtgctcgcgc tggccaaact ggaagcggtc tacaaagaac gcggcatttt cccctgatag
    12481 cagcaccggt cgcatgggcg acgtggcccg gagaaggaat gcgattgcgg acgcaaatct
    12541 gtcgacagat tgtcaacaag ccgcactccg ggccaacgcg ggtgcggaga accgacgaat
    12601 ctccgtggaa ggcggcctgc gcctcggata accgtccggg gcgttaggcc gctgcgggca
    12661 acgcgccaga aggttttcgg gaattcgttc aggagatact gggatgaaaa ggcgcaacct
    12721 aggggatttg cgtgagtggg gccgcgtatc cgaggagctg gccgccatcc agcgccaggg
    12781 gggcctggat gagtaccagc aagaacttgt gcacatgctg cggttccgcg acaactggcg
    12841 cctgcgtgag atggctctga ccagcattaa gcgtgtcgag gctgtgtcgg aggagctcgc
    12901 ccgcgaaatg ctcaaaataa tgaacgacga cgaattgtac gacgaagtcc ggatgctggc
    12961 cgcggaggcg ctggcggacg ccttggcgcg ggcccgggcg gcgaatcgca acgcgctagc
    13021 cggcgtcgtt cgcgatgcaa tcgacactat gcatgcgatc cttgacgggc cgcagccacc
    13081 ggtattacag gaaacggtcc gacgtgcgct ggcggcgctg gagtaggtgc cgcgcctatc
    13141 gcggcggtta aaatttggaa agaacggaaa caaccatgat catcggcatc ccaagagaaa
    13201 taatgccggg ggaacggcgc gtggcggctt tgccggagac ggtccgcgaa tacgtcaata
    13261 tgggctttca ggtgctggtc gaggcgtccg ccgggcgggg cgtcttccac agcgaccagg
    13321 actatcgtgc cgcgggtgca gagatcgtgc ccgacgccag cacgctgttc gcgcgggccg
    13381 acctggtgct caaggtgaag caaccctgct ttaacgagac cgtcggccgg catgaagccg
    13441 aactgattcg cgaaggggcg ctgctgataa ccttcttgca cccggccgcc ccggccagcc
    13501 acagcgtcgt ccgcacgctt tgcgaacgta acatcaccgc cctgacgatg gacggggtgc
    13561 cgcgcatctc gcgtgcccag accatggacg cgctgacttc aatgagcacc atcaccggct
    13621 acaagtcggt gctgatcgca gcctcgcatt ttccacgctt tatgccgatg atcggcacgg
    13681 ccatcggcgc gaccaaaccg gccaatgtgc tggtggtggg caccggcgtg gtcgggctgc
    13741 aggcgatcgc cacggccaag cgactgggag ccactgtgac ggccgtggat atccgcccgg
    13801 acgcgcggcg tgaggccggc agtttgaaag gcacgaaggt cgcgggattc gaggttccgg
    13861 acgaggtggc cctggcgccg ggcggctatg ctcgggcgct gccgacggag tggctggtgc
    13921 gcgaacgcgc gctgctggat actctgctgc cgcagaccga tatcgcgatc ttgagcgccc
    13981 tcgtaccggg cgaggtcgcc cccatcctga tcacggaaga agcggtccgg acgatgcggc
    14041 cggggtcggt gatcgtggat gtggccatcg accagggcgg caactgcgcg ctcacgcagt
    14101 tcgggcgcga ggtggtggtg caggatgttt acatttgcgg caccgccaac ataccgggct
    14161 cggtggcggt cgacgcgagc tggctgtatt ccaacaacat gctgcgctac gtgcaaaacc
    14221 tgttcaaaga cggcccgcag cgcccctgtt tcgaggacga gatcgtgaag caatcgctgg
    14281 tgacgcgcga ccgcaagata gtgcatgccg gcacgctgaa agccatgcga ctcggataaa
    14341 caggctcagg agagcggcgt gaacgaagta ctgctgatgt tgggcatctt cgtggcggtg
    14401 tttggcttgg gctacctgtt catcacgcgc gtgccgcaga tgctgcacac gccgctgatg
    14461 tcgatgacca acgccatttc ggccgtgacc atcctcgggg cgctgttgct gttttcggtt
    14521 gaaaccagcg cctttgagaa ggtttgcggg gctgccgcca tcatcacggc cattttcaat
    14581 gtggccggcg ggttcgtcat tacggaccgg atgctgggca tgttccggcg caagaacgtc
    14641 gccccgttga agttcagcaa cacgggagac aattcacctt cagggcagga ttgagctatg
    14701 gtgtcaggtc tgctggtcga tcttctggtg attgccctgc tcctggctgg ggtttggcag
    14761 tttcgcactc cgccgggcgc gcggcgcggg aactggacgg cggccgttgc gctggtggtg
    14821 gcgaccgtgg tggtcctgtg gcggcacggg ctcgagagcg tgccgctggt ggtaatcgtg
    14881 gggctgctgg gaatcgggtt gggcgtgtgg gtggccctgc gcgccacgat gcttcagatt
    14941 ccggccctgg ttgccttcca gcacggcatg ggcggcgccg cagccctgct ggtctccttt
    15001 gttgagctga cacgcgacgc taccaaggca gccattaccc cacctgaggt ggcggctttg
    15061 ctggggctga tcgtcggggc cggcacgttc agcggcagcc tgattgccgc cggcaagctt
    15121 gaccggcgcc tgccgcagac gccggtgtct ttgccacgcc acgacgccat gctgctctgc
    15181 ctggcgctgc tggtggtcgc ggccggcatc gttgccgctc tggccaagga tatcgcgctg
    15241 ctgtcaactg tgctcgtggt ggtggtggcg ctttcgattg ccatcggcgc cttgttcgcg
    15301 atccgcatcg gcggcgctga catgccggta ttgatctcat tcctgaacgc caccgcagga
    15361 gtggcggccg ccctgtgcgg cgtggcgatc ggcaaccgct tgctgatcgc ctgcggtgcg
    15421 acagtggccg cttcaggctc gatactgacg cacgtcatgt gtcgcgggat gaaccgcggg
    15481 ttgacctcga ttttcgcagg aacacgcagc agcgcgccac cggccggcgc cgcacccgcg
    15541 ggcgcgtccc atagccgtcc cgaacccgcc gcccctgccc cggtcagcgt caaaacttca
    15601 gcagcgccga ccgacgtcag ctcgaatgga gacccgtaca accacgccgc cacgctgctg
    15661 cgcgaggcgc gacgcatcat aatcatcccg ggttacggga tggcgttggc cgaagcgcag
    15721 gagggcgtcg tcaaactggc cgagcaactc ctggagcagg gcaagtcggt caagtttgcc
    15781 gtacacccgg tcgccgggcg catgcccggg cacatgcacg tgctgctggc cgaggcggat
    15841 gtcgattact cgctgctgtt tgagctgaag gacgtcaacc acgagtttgc ggaaacagat
    15901 gtagcgcttg tggtcggggc ctgtgatgtc gtcaatccgg cggcgatcga gcgtcccgat
    15961 acgccgatct ccggcatgcc gattctaaac gcacacgaag ctcggcacgt gatagtgtgc
    16021 aacctggacg agcgccccgg ctactccggg gttgagaacc cgctgtacaa aaatccgaag
    16081 acggtgctgc tattcggcga tgcgcaagaa agcgtcgaga agctccgcga ggggctctcg
    16141 ccagcgccga ccgacgtcag ctcgaatgga gaccagtaca accgtgccgc cacgctgctg
    16201 cgcgaggcgc gacgcatcat aatcatcccg ggctacggga tggctttggc cgaagcgcag
    16261 gagagcgtgg tcaaattggc ggagcgcctg ctggagcagg gtaagtcggt caagtttgcc
    16321 gtacacccgg tcgccgggcg catgcccggg cacatgcacg tgctgctggc cgaggcggat
    16381 gtcgattact cgctgctgtt tgagctgaag gacgtcaacc acgagtttgc ggaaacagat
    16441 gtagcgcttg tggtcggggc ctgtgatgtc gtcaatccgg cggcgatcga gcgtcccgat
    16501 acgccgatct ccggcatgcc gattctaaac gcacacgaag ctcggcacgt gatagtgtgc
    16561 aacctggacg agcgccccgg ctactccggg gttgagaacc cgctgtacaa caatccgaag
    16621 acggtgctgc tattcggcga tgcgcaagaa agcgtcgaga agctctgcaa ggggctctcg
    16681 gcaagcgctt gaccgaaagc gcgccgacgt actcatcaaa tgcggcggac cgcactccag
    16741 ccaggctgga gtgcggtccc tttttgtcgt catactgtcc gcgcgtcacc agcgccgcag
    16801 cgcgccaact atgacccggg cggcccggtt gggccgcccg tcggcattat ggagacatcg
    16861 ctttcctatc ggttcccggg cgggccttca gtttgaacgc cgaaagcccg aacttcccag
    16921 gctattccag caactgcttg cacctgtcgt acaattccgg ctgctgctgc ttgaactctt
    16981 ctttgctttg gaactcgtac gtctgcggac cctggccatc atccactgtt accgtgacgt
    17041 gcccgtcctc gacctgcact gacaggcgct gcatcttctt gatcactgtg cgctgctcgt
    17101 cgaccgagcc agagctgtcc gtcgcattgc tgcggtattg ctccagacgc tcacgcacgc
    17161 gctcgactgc tcgggcggcc tcctcctccg cggccttggc ccggtccaga gctttacgca
    17221 gcgtggcttc aatttcgacc tggaagtcct gccgcaactt gttcaggtgt tcgcgttgcg
    17281 ggtaaacgaa attccaggac tcgggaccgc ggcttgagaa cggccggtag gctttgtagg
    17341 cgtccgggtc cgcttttcgg aattcatcca tgtcctcgta cgtggcggac gattcgttgc
    17401 cggcgggatc gacgcgcttg acgttaatcc tgccgtcttt gtcgcgccag atggtgagcg
    17461 tgttgccgtc gtcatccacc tggacgcgca cctcgacgcg cgccttgacg tccagatcga
    17521 catcctcctc ggcgaatgga tcgatgctca cgtcgaagtc gggcaggtgc agctcccgca
    17581 agatgtctgg gaacgtgtgc agcggaccga gctcttccca ttgccaggcc ccgcccggtc
    17641 cgggcatcaa cttgtggccg cgcagccgca tctggcgatc gatgatgtcc tccggttcgt
    17701 cgtacttcca ggttacttgt ggggcggtag ggcgttcggc cggggtgata gtcatttctt
    17761 tcttctgcgc gccgcgaatc acggtcagcg ggacgctctg gccgggttta atatcctcca
    17821 gcgcggcgaa caggtcttcc gagtcggcca cctgctgcga gtcgagcgaa acgatcacgt
    17881 cgtagcgttc aacgccggcc cggtcggccg gactgtcctg cacgacgttg gcgaccatga
    17941 cgccgttttc gccgatgtgg gccgcgagcg gttcgggaat cggcgttagc cggacgccca
    18001 gccactgcga cggcattttc tggatgctgg gatttggggc atagggatca cgaataagca
    18061 agcggcgcga actgatcacg gtcggcgggt cggggtccgg cggcgcagga gggccgggtt
    18121 gaaaccggac taggtgtacg caatcgccgg gctgcgccgc gggcggcctg gacagcgccc
    18181 accctgtcac ggcttgtgcg agcaggatca gtgtcagccc actcgatacg gtcatcattg
    18241 gaatactcct ctagaagtcg tcctggatcc ggatggctcg cgtgcgcagc cggtccattt
    18301 gaatcagtag aaattgcccg ttctgccggg caggaacaac gatccagtgg cggtcggtat
    18361 cacgccaccg cacctgtggg cggacgtcgg ccgggtccac ctgatcgacc acgtccaccg
    18421 ggcgctccgg cggtgcgaac cacgacgcgc gcatcggggc cgacggctgc ggcctttgaa
    18481 ctgagtgctt cggtccattg ctcggcctcg gcgagctcag aaagaacacg agcccgatgc
    18541 acgcggcggc cgccgcgaaa ccgacggcct gaatccaatg cacctttttc acgcgccgcg
    18601 ccggcagcgg tagggtgcgc cgcatggcca ttcgcatggc acgcccgacc tcgcgatcga
    18661 tggcggccgt ctcggccagc aacgactgga tcgccgcgtc ggcattggcg cgcgattcga
    18721 gatcgcgccg ttcagccggc gtcgcctcgc cgtcgaggta gcgcgagatc aacctttcca
    18781 gctcaggcga accgtcgttc atacgtccac tttcccgtac aacatttcac gcaatagccg
    18841 tcgggctcgt actaaccgcg tttccaccgt ctccaccggc aggcccaggc agtcgccgat
    18901 ttccgcgtag ctccagttct ccaggtgccg cagcacgaat ggctcgcgat acagggccgg
    18961 taaggcttgc accgcccgca gcagggttgt ctgtagctcg tcctggagaa cggcgccgac
    19021 cgggctggcg ttgtgcgcgg cggcggcttc cggcagcaca tccaggctgc cggtccggcc
    19081 gtcgcgccgc cggcgggcct ctccggcgtc gatggccgca tgccgtgcca ccgaatacag
    19141 ccaggatcgc aagcggtgcg ggtccttaag cgagtccaaa tgttcccaaa cctggatcca
    19201 cacctgctgg acgacgtcgt ccacccaatc gggccggccc gtgatcccat agaccacgct
    19261 gcgcagccaa gagtcatgct cgcggaccag gcggtccgcc gcgggccgat ctccgcgctg
    19321 cacgtcggcc acgagttttt gcagcgcttc accgtccacg gtggcagcga ttgtcactgg
    19381 aggatcagac ggtttgctca tgccggctac gccaaaaaat tgtgtcaggc gacgcaaaat
    19441 ggtcccgccg tggagccttt ggcgaaccgg gcggcggcct ggacaccggt tgtaatggtg
    19501 atcgctgctt cgtagtattc gctctggccc gtggccttat tatggagcct taatatgccg
    19561 ctgcccagag tcttaaaaga ccttctcagc ctgccgaccg ggacgttcgt cgagggagcg
    19621 gtcctggagt acatcgaaag cacctgctcg aacttgaaag gcgttacgta cacctccgac
    19681 cgatacggca atatcctggc tcattatcgg aatcggccgc ctggctcgac gccgctggtt
    19741 tttgcggcac ataccgatca tccggggttt gtcgcccagg agatggttga cgcgaagaca
    19801 gtgcgggcgg cgtttcgcgg cggcgtggcc tcgtcgtaca tggagaaagc gaaggtcagg
    19861 ttctggagcg gcggacgttg gattaacgcc aaagttcgcg agctgacccg caccgtggcc
    19921 gctcctcgct ttggcgccgg cgcgacgcgc ccggaagaag gcttgcttac tgtcgcccag
    19981 ccggttgagc caaattcgcc gggcatgtgg gatctgggcg ggcccgttct cgacggcgac
    20041 ttggttcgcg gcatagcctg cgatgatctg gctggcgtgg cggcgatgct ggcgctactc
    20101 gcccggctga gcaagaagca ggccgcagcc gaagcctact gtctcttcac ccgggcagag
    20161 gaggtggggt tcatcggggc gatcggtgcg gccgaggcgc ggacggtttc gaagaagctg
    20221 ccgatcgtgg cgattgagac gagcaaggcg ttgcccagcg cgcccatcgg cgcgggcccg
    20281 attctgcgcg tgggcgaccg ggccagcata ttcacgccgg agctgaccgc ctggtgcggc
    20341 caggtggccg agaagctcgc caagcggcgc aagggcttca agtttcagcg caagctgatg
    20401 gacggcggga cctgcgaatc caccgcttac gtcacgtacg gctacgacgc gaccggcttg
    20461 tgcctggcgc tagggaacta ccacaacatg aacgagaaga ccggcaagct gggcagtgag
    20521 tacgtcagct tgtcggactg gcggagcttg gtggactggt ttgaggcgct ggtgcttgac
    20581 ccggacgggt ttgcgccgac ggagtcgaag aacatggcgc gcgtcg
//
LOCUS       DAOSYS010000035        20575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2129_length_20575_cov_5.608821, whole genome shotgun sequence.
ACCESSION   DAOSYS010000035 DAOSYS010000000
VERSION     DAOSYS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20575
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_20575_cov_5.608821"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLU99_03635"
     CDS             <1..783
                     /locus_tag="PLU99_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16178.1"
                     /translation="RLIDFALLTVGGPYLFVTLLAAAIAFRPPPAAQPAPHRKWLVAV
                     AAILLVFFQLTGVTLPTMRTEFWLQQLRLIAFSPPGFAGRDDIRALEQRVLASDPLDA
                     EAARQVAGWAMLIGTGESGTQERLKLLKQAEELGLLALERNPHSRAAEVRLGKLYEDM
                     QEAYLLALLPDEAGEALHQAALHWQRAVELYPTDPRTRISAGKVWLEWSQESDDPQAR
                     AIAAEHFNAAREIDDSRPPGEVMRLRPAEREELEQYLQELQG"
     gene            931..2280
                     /locus_tag="PLU99_03640"
     CDS             931..2280
                     /locus_tag="PLU99_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16179.1"
                     /translation="MDAFQAGGHAGDVQDGAISRRRFCQAAALGLGGLAIGEYLSGFQ
                     ARAAGPGPLPPPIVTGRSLEICMNSRVSRHSGLTGTATTQQISNVLWAAGRAPVVGSH
                     RTITLKTPQATYIYHPEDHTLEYSSSSTVSNAFRLNFDRELDFDAGVAYVLALWASVS
                     LWTGTSSQLVSCPQIEDLNFGVNSCAGLTTSLAAVSSDGSLPNPITDGNNRLEEVLAK
                     LRLRHTFRPDLDLAPQRLSQILWAGYGNAPHTAVGRGGLTVPSWTASYFLTNRIYVVN
                     DRVWRYCNRIGSDPATRDHRLQLVKNSDVREQLRTALPDLPHAPCYVLLCLSSTGLTT
                     WYQRLETGFVAGGILLQGAALGLGCDFKVPLDSSEQAAVKSIAQLPSGDYPHAIVAVG
                     HPPADLDGDGDLDGADVSMLGVCLNGPEVAASPDCAGADRNGDGDVDLSDFAGIQPD"
     gene            2729..3613
                     /locus_tag="PLU99_03645"
     CDS             2729..3613
                     /locus_tag="PLU99_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16180.1"
                     /translation="MKRLIAVVAASAAMLALNVQAQDCIIISEIVHGAGSGGCPVWME
                     ITNTGTSDYTFPAGGVIVQTPPGTSDLIVDIDLTGVTISAGDSFVIATRNGYWCTGSF
                     FGTYGFDPDMTVNSVWGETQDGEGDAALILTDTADGSHLLDIYGEFGVDGTGLPWDTT
                     HGYSHRLPAYSSGSGGTFVASEWFLGGAESSVPPVMEPPISDEEWLQMVTTPGTHEFE
                     PCSPCGAQQKADANCDGAVNVFDIDPFVLALTTPEAWEAEYGSSCNLLCTCDCNSDGS
                     VNVFDIDPFVSFLTSGSY"
     gene            complement(3732..4952)
                     /locus_tag="PLU99_03650"
     CDS             complement(3732..4952)
                     /locus_tag="PLU99_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOO16181.1"
                     /translation="MTAHPSGAPAATALPSRAPATRIAYVDVAKALGILLVFYGHLIE
                     CFFRSVRQGDLVEWRQAALEQWRWIYCFHMPLFFFLSGLVYKERSITAETFLKRQTLS
                     RLTPVWVFNFISMVLVVFIEFWTRSNPATQLATIFKAKAVRFGWETLCGRPFFNVLVW
                     FLVCLFVVELIQFALRKPFRRTLWLVVSMAVFAGITHVFTCYRSQIADIWGSLPFDMG
                     LLKIDFWQFSSVVAAIVFYQGGILFQRVTAMGLASRRLPLVAAGAVGLVVTLVTLNYN
                     KVHTVAYVAHGAYGNVGWFFLTAFAGILFMVGLSSLLSFSGILARFGQITLGLMCLNG
                     ILHGLVNLPLAQRVAPLLPKDSVLILTATCVGLTLLSVAVCLPIVWLIERYVPVVLGR
                     WPSRTPSKAASAAN"
     gene            complement(4949..5185)
                     /locus_tag="PLU99_03655"
     CDS             complement(4949..5185)
                     /locus_tag="PLU99_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16182.1"
                     /translation="MAKRHGGPRRPSGSQAWMPLLAALLGLLTTISLAWLLAITRAHL
                     GARETVCAPAAAVTNQHALQPGSARGWSHSKDKP"
     gene            complement(5234..5923)
                     /locus_tag="PLU99_03660"
     CDS             complement(5234..5923)
                     /locus_tag="PLU99_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16183.1"
                     /translation="MSADAARAAHEMAGSNSAPGLSSAPGLSPTGGFLARFRRSDTRA
                     AQLATYQRMAEMVESLREHYRQQDERAARVEQQTERISGILQQLADDGHRQQEHVRAM
                     ADNVSLASRHTAAMSETLSLLPPLLQAEADAVRSMVRRMDVAQETDTQLMHSLQKLGQ
                     AVEALQAAGAAQVQTLERLNAAEREQRHALTLLVREQTRRFLLIMLVAAILGLGALAV
                     LSAVLITRLYH"
     gene            6236..8170
                     /locus_tag="PLU99_03665"
     CDS             6236..8170
                     /locus_tag="PLU99_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16184.1"
                     /translation="MSTITDRRTRLTERSSRRQVVTVPWWQRDMVLAAAVFVLAVAVF
                     ANSYVNDHVYDDIGIIATNTRLDWPWDLKALFATDYWGEKLTNSKLYRPLTILTLAVD
                     CALFGKDPPRGIHANNILTNAVIAVLVYALIRRLWQRRDLAFVTAALYAIHPVHTEVV
                     ANGAGRSELYMVLTLLAALHLHLTYARRRFERDEKTSRTQAGQPKPRTAPQPRIYPYL
                     AGAAVLYFVALLFKESAVVLPALALLIDLLVVRRGRRPAWRYWLAYLAYLPGLVIYTT
                     IRLAVLGPALPAVQEVMSSATGWQRFLFASETMLRYIGQLILPIWLCGEYADFTRIIR
                     PSVTEPMVLAGLAAWFVWAGLIAWLARRRHYLILTGIAWFFISIFPVSNLIVPVGTIR
                     ADRLLFLPSLGFAMIAGWALVRLAGVQRAVGIVATLGLLGFYGVRSALRNLDWRDQDT
                     FWTKTISQNPGSAVGWLAIGHGYNVRNDYVQAAAAYQRAYQLRDSAGFFYEDAHLDCA
                     LMLEALGDLKGAAAEYRAVLEQYPKHKAALMNLGQISHRDPATRTEALELFKRYVAEE
                     PTDFRGHANLAQAYKYARQYAPALEAIERALALERGGPDEGKLLLVKADILRLSGNFE
                     ESERVRALGEQRQAAQNRGG"
     gene            complement(8171..9880)
                     /locus_tag="PLU99_03670"
     CDS             complement(8171..9880)
                     /locus_tag="PLU99_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16185.1"
                     /translation="MKWPLVFWFALASVCIARAGDRLDRALSHVPEESALIIVAPSLD
                     KLVSGLAEFGGSIGVERLRTLGPAKLLNELDVLERINGLDPAGPLVIALEPTRLSPLL
                     VCQVLDAEEWRTASGAQPADGELWRVQMFGEPAYAASAGNLLIVGDEPDAVRSALRGS
                     FDYAARFGPRARAWLSDHQLVVAIRVPPWTGLISPMLGAGRTSLQLSMMLAGQRDENL
                     LRVVGWLFQQAEQLLGQVDTYTAAVRIDSAGVFFRDALSFAPDSAAAAYLKKVIKTDR
                     DLLRGLVDDETVFVFAHEWELEPGTEGLNTAVVAALLGSGTLRERLGEEKFLPGYEAL
                     LAAYNDISGSCTAVSARPAEPLLVFSAVQFSSRPQQVLSNMRLAYETMPEFLSALGGG
                     ASADVTCRAERIESIDAFACEVSFAAPDEQSRRAIEMLYGRTLTTYYAAQPVGLVSAT
                     ASAERAAAQLVRMTSGKGRPLAENPRVRQAWARLSPRPQVLLLLDAAKALDLVLRVDR
                     SGRTGTTAPAPDTSLPLVAYGVYLDGRTLCSELCVPAEAAKQIVKTIQSQKEAARGPG
                     RQP"
     gene            10197..10850
                     /locus_tag="PLU99_03675"
     CDS             10197..10850
                     /locus_tag="PLU99_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16186.1"
                     /translation="MSDGEPDATAVLESSQPGEQCEPTAPEPHDAIPVVPVPVPAHVQ
                     QPKPRAPAVTAGPEQQQQAQQQQAPSATTPSHPSEPPLSHAEGQEGPRTTLQRILHAL
                     RPGRRKASRAQNAAEISEPAAGARPAAIVVVLDLVLSLINRPFAFLSPGARHVVGLVA
                     IVTLAIAAVTAALAPLAPKNDAVTFIRQKRAELDQPPPPPAAQSEKPQAENAHGAGH"
     gene            complement(10904..11695)
                     /locus_tag="PLU99_03680"
     CDS             complement(10904..11695)
                     /locus_tag="PLU99_03680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO16187.1"
                     /translation="MQRREKLHNAAMMPASRVKSRPANRKPTSAWHAYVEDAQRPLYC
                     LLFLFPLVAAYEFGALLLRPVSCPGRQLVAHSLIQNLLGWFGASGTLLPALALLATLL
                     VWHLLSGQGWRVRGWVLPLMACESVVLIVPLFVLGRLLLAAPSSAANLADLRQQMVMA
                     LGAGLYEELVFRLGVVCAVLWLFENGLRIRNAAGPCTAVVVSAAIFAACHFQPIGIEP
                     FELTQFLQLTAAGAYLAVVFLLRGLGITAGCHAAYNLVLVLSGPL"
     gene            complement(11702..12685)
                     /locus_tag="PLU99_03685"
     CDS             complement(11702..12685)
                     /locus_tag="PLU99_03685"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOO16188.1"
                     /translation="MSRLAGLLLMLGLTAGQSSAWQASSVAPEPGASFCSEHFVIHAD
                     SEAQARALAPRLEAVYRANVRLARELRLDVKPPAGKLAVLLLRDYAGFHSRLRADGLD
                     EQILGYFSPAEARCVFFDFNTHPEVTGLRQELDKLHRQDEGRRRRLAAQIEQSLAGMN
                     QRILQHEAAHMVQANIGLIPPDSNSPTWLVEGLAEMFELPFVETGASLGLSVNRYRLR
                     EFQQLQKGDDLLAALRRLVATASWHGGADYSLAWALTQYLYVRQRTNFGAYVRAVTIA
                     GKAPAGGALEQFENIFGPLDGEFAARFAAYMEGLIGRYLAPQDEVRSRAAP"
     gene            complement(12739..14073)
                     /locus_tag="PLU99_03690"
     CDS             complement(12739..14073)
                     /locus_tag="PLU99_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOO16189.1"
                     /translation="MKHARRWGFGLLIALLLAACVLPEVQAENLGKMRIQQTDGKWVT
                     GDVEETPEAYLVKVGSITVTIPKTRVKAILPVEETQSPGEAQEESKHGSGLKDLEIEK
                     LLEGTGLMPPDEDPDDIELPLDEESVGEMMRIAGTENIYATKHVVVVYTSTKQKAAEI
                     ATRLEAIWRWRVRFMKMLNLPLRMPEHKLELYYFADHEEFKTLQVNIGGQPDDGTLGF
                     FSPDSNRSHFFDMLTWRPIKMRLQSLKNAPASVRAREENMARQWAEYQTLSVTQHELG
                     HHIDFNLGLFPQAVFLDMESFDSIPRWLVEGTTMMFEVPPTTEGASLGVINYERVEQF
                     QTNPNFAQKWSPDFFKLFVVRNEIWLQTGWYYPMGWALVYYLWEVKRPGLIEYYHIIS
                     SRDPGEAVSYSQREREFQDCFGTIDQKWVDDFWKYIMELPRKRSLLPPKDYP"
     gene            14240..15196
                     /locus_tag="PLU99_03695"
     CDS             14240..15196
                     /locus_tag="PLU99_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOO16190.1"
                     /translation="MDHVRGGETAFISRLRAELPAPLRLARPLGRDHRPLDFLDDMAA
                     VAGPGSDLLWTADMLMDGVDFDSRRHAWALIGRKAMAVNLSDCAAMGVRPVSALCAVA
                     LNNQLSMDDAVALVRNAHNMGLAFNCPIVGGDTNSWDAPTVISITIAGQPETGQHPVR
                     RDGARPGDLICVTGPLGGSILGRHLTFEPRVELALQIVRSLAPHAMIDISDGLALDLH
                     RLLEASQCGAILDAAAIDGLIHDDAHALAAQDGISPRQHALFDGEDFELIVILDPAAP
                     AAELAHLRLSPIGRIVPGAGVLLREADGRELTLERRGWEHFK"
     gene            15193..15681
                     /gene="tsaE"
                     /locus_tag="PLU99_03700"
     CDS             15193..15681
                     /gene="tsaE"
                     /locus_tag="PLU99_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOO16191.1"
                     /translation="MTPTIDFSSASPEETVRLGEAIAAAVQPGDVLALYGPLGAGKTQ
                     LVKGLAAGLGVPPDEPIVSPTFVLVREYAGRLRLYHLDAYRLSGSDELAGLGWEEMLD
                     DPHAVVVLEWADRVAAAIPPAAWRIDLEHAGPEARTIRITCPDARRAAPLARLLAPSR
                     GP"
     gene            15891..16862
                     /gene="trpS"
                     /locus_tag="PLU99_03705"
     CDS             15891..16862
                     /gene="trpS"
                     /locus_tag="PLU99_03705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOO16192.1"
                     /translation="MRILSGIQPSGTLHLGNYLGAMKQHLELQTQHECFYFIANLHSL
                     TTIHDAERLRKLTLDVALDYLTLGLDPQRVTFFRQSDVPEVTELAWILSTVTGKGLLE
                     RAHSYKDKMAKGLPVNMGLFSYPLLMAADILIYLSNEVPVGRDQVQHIEMTQDMAEYF
                     NNTYKPIFIRPEPRLNEAPVVPGIDGQKMSKSYGNIIEIFGEPKAMRKKIMSIKTDST
                     PVEEPKHPETDNVFALLKLLASPEETAEWERRYRAGGMGYGEAKQRLFELYEAKFGPL
                     REVRADWAKRPDEVEDILSTGGRRARAIARDVMAQVRDACGIVVSRE"
     gene            16984..18168
                     /locus_tag="PLU99_03710"
     CDS             16984..18168
                     /locus_tag="PLU99_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOO16193.1"
                     /translation="MSEGYRVQLEVFTGPLDLLLYLIRRDELDIQDIPIARLTEQYLA
                     YVHVLEQIDPNAAGEFLVMASTLVELKSRALLPTPPLEPMEDDASDPRTTLVRQLLEY
                     KRFKDAAEELRRAGEERAQRFPRSPANLPKELQGIELEEAQVWDLLEAFGRVMTAIGR
                     GPTQHQVQYDETPIELYQAEIVALLGEGVATFQSLFDGQSTRGEVIGRFLAILELVRS
                     GRVRAEQDRNFGTIYLFLADPREPEGEVEPGPELAEPAPDAAPSAGEAGLSANDARPS
                     DGEADLTASEARLSASEAGLTTSGAPPSASQAAPRTSEARLEATAHEADSAESDSADA
                     DVPGLEPAEEAACAAESPETPPAPEQSLAVSDRMSPAAESCETCSTEHSNEPDQSTLE
                     GP"
     gene            18134..18601
                     /locus_tag="PLU99_03715"
     CDS             18134..18601
                     /locus_tag="PLU99_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006897370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO16194.1"
                     /translation="MSQTSQRLKDLNITLPPVSKPVGAYVPALRHGDLLMLSGQIPLR
                     EGKVAFTDKVGGPAGRTLEDAQAAARLCTLNAIAVAADAAGGVDRIKSVLKLVVYVAS
                     NPGFTDQHKVANGASDLLVEVFGEAGKHARAAVGVAELPLNSTVEVDVTFVVS"
     gene            19944..>20575
                     /locus_tag="PLU99_03720"
     CDS             19944..>20575
                     /locus_tag="PLU99_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16195.1"
                     /translation="MSRLYAQVAVTPSAPARRAHLLCATVLLSVTVLLSAMLTSCSRS
                     NEQEPPARSSPRETIARLIAAREQRQYQVMRELIVPEHARDVIATLAAVDDFLSVNDQ
                     LCDLIRSEIGLGTADAIDQGQLAYHLDVFSRNVKVLDESIAGHDARVAFLVDERLPAR
                     HAQLRLLDGTWRYDPGAGEFAKLSQAFERMAHGLRQVLEGLRRGQLSAAR"
BASE COUNT         3747 a         6816 c         6379 g         3633 t
ORIGIN      
        1 agattgatcg acttcgcgct acttactgtc ggcggtccgt atctgttcgt gacgctgctg
       61 gccgcggcga ttgcatttcg gccgccgccc gccgctcagc ccgcgccgca ccgaaagtgg
      121 ttggtcgccg tggccgccat attgcttgtg ttcttccaac taaccggcgt gacgctgccc
      181 accatgcgaa ccgagttctg gctgcaacag ctcagactga ttgcgttcag cccgccggga
      241 tttgcgggac gcgacgacat ccgcgcgctg gagcagcgcg tgctcgccag tgatccgctg
      301 gacgctgagg cggcacgcca agttgccggc tgggccatgc tcatcggcac cggcgaaagc
      361 ggaactcagg agcgcctgaa gctgctgaaa caggcggagg aactggggct gcttgcgctg
      421 gagcgtaacc cgcactccag agcggccgag gtgcggctgg gcaaactgta cgaagacatg
      481 caagaggcat acctgctggc cctgcttccg gatgaggcgg gcgaggcgtt gcaccaggct
      541 gcactgcact ggcaaagggc agtcgaactg taccccaccg acccgcgtac tcgcatttcg
      601 gctggaaaag tttggctcga atggtcgcag gaatcggacg atccccaggc gcgggcaatc
      661 gcggccgagc acttcaatgc ggctcgtgaa atagacgatt cccgcccgcc gggcgaagtg
      721 atgcgtctgc ggcccgccga gcgcgaggag ctcgagcagt acttgcagga gctgcagggc
      781 tgaaattctc gcttcgctgg cggcagagtg ctatgctgcg cttggctcgc gagggcattg
      841 ttggtggtgg catgcccgcg cttagcgctt gggcatggca cccggcgttt acagcttggg
      901 catggcaccc aacgccggtg gaggtggaag atggatgcat ttcaggcagg cggacacgca
      961 ggcgacgttc aggacggggc gatcagccgt agacgatttt gccaggccgc ggcgctagga
     1021 ctgggcgggc tggctatagg cgaatacctc agcggctttc aggcccgggc ggcaggtccg
     1081 gggccgctgc cgccgccgat cgtgactggc cgctctctgg aaatctgcat gaatagcagg
     1141 gtttccaggc attccggcct gaccggcacc gccacgaccc agcaaatctc gaacgtctta
     1201 tgggcggcag gaagagcgcc ggtcgtgggc agtcatcgca caatcacgct caaaacgccg
     1261 caggcgacgt acatttatca cccggaggat cacacgctgg agtactcctc ctccagtacg
     1321 gtgagcaatg cattccggct gaacttcgac cgcgagctgg attttgacgc cggtgttgca
     1381 tacgtgctgg cgctgtgggc ttcggtctcg ctctggaccg gcacctcatc acaactggtg
     1441 agttgccctc aaatcgagga cttgaacttc ggcgtcaata gctgcgcggg cctgacgact
     1501 tcgctcgcag cggtgtcttc cgacggctcg ctgcccaatc cgatcaccga tggaaataac
     1561 cgcctcgagg aggtgctggc gaagctgcgc ctgcggcaca cgtttcgccc ggacctggat
     1621 ctcgcgccgc agcggctctc gcaaatcctc tgggccggct atggcaatgc accgcacacg
     1681 gctgtcgggc ggggcggttt gacggtgccc tcctggacgg cgagttactt cctgaccaac
     1741 cggatttacg tcgtcaacga ccgcgtgtgg cgctactgca accggatcgg ttccgacccg
     1801 gccacacgcg accatcgcct gcagttggtc aagaacagcg acgtgcgcga gcaacttcgg
     1861 acggccctgc ccgatctgcc gcatgcgccc tgctacgtcc tgctgtgtct tagctcgacc
     1921 gggctgacga cctggtatca gaggctcgag acgggtttcg tggccggcgg catcctgctg
     1981 cagggcgcgg cgctcggctt aggctgtgac ttcaaggtcc ccctcgactc aagcgagcaa
     2041 gcggccgtaa agagcatcgc acagctccct tcgggcgact atccgcacgc catagttgca
     2101 gtcggccacc ccccggccga tctcgatggc gatggcgacc tcgatggcgc cgatgtaagc
     2161 atgctcggcg tgtgcctgaa tggtccggag gtagctgcca gtcccgattg cgcgggcgcc
     2221 gaccgtaatg gcgacggcga cgtggacctc tcggacttcg ccggcattca accggactga
     2281 gattagcggt cctccggcag tgacgtgtgc tagcggaatc taaaaaccaa tggcccgcgc
     2341 cgctgcctgg cctccacagc cgagccttaa cgacgcgcgg agcactgccc gtgcgacgct
     2401 tgacccatcc gtcccagaat cccttatccc cctcgccgcg gccaaccgct caccagggac
     2461 ccgaaaacac cggatattat taggtgcctc gctcgggctc gtcctcgccg ctctgcatct
     2521 ttgcgcctct taagccgatt gaattcgccc ccccaaaacg attatcatga acaggtcggt
     2581 tgctcggcag aaaatccgac ctcggcgccc agcgcagccg tggagtgctc gctgcgctga
     2641 ggcgattccg ttcccgcgcc ctgccggcgg ttagcgaacc gtcggggcgc gcttgtcgaa
     2701 tttggctttt caggaggatg gaaaagtgat gaaacgtcta attgcagtag tcgcggcaag
     2761 tgccgcaatg ttggcgctga atgttcaggc tcaggattgc atcattatca gcgagatcgt
     2821 gcatggcgcc ggcagtggcg gctgcccggt ctggatggaa attacgaaca cgggcaccag
     2881 cgactacacc ttcccggccg gcggcgtgat cgtgcagacg ccgcccggca cgtcggacct
     2941 gatcgtcgat atcgacctga ccggcgtcac gatcagcgcc ggcgactcct tcgttattgc
     3001 gacccggaac ggctattggt gcacgggttc gtttttcggc acgtacgggt tcgaccccga
     3061 catgaccgtc aatagtgtct ggggcgagac gcaggatggc gagggcgatg cagccttgat
     3121 cctaaccgac accgccgacg gctcgcacct gctggatatc tacggcgaat tcggtgttga
     3181 tgggaccggc ctgccgtggg ataccacgca cggatactcg caccgtctgc ccgcgtacag
     3241 cagcggcagc ggcggcacgt tcgtcgcttc cgagtggttc ctgggcggcg cggaaagctc
     3301 ggttcccccc gtgatggaac cgccgatttc cgacgaggag tggctgcaga tggtgacgac
     3361 gcccggtacg cacgaattcg agccctgctc gccctgtggc gctcagcaga aagcggatgc
     3421 gaactgtgat ggcgccgtca acgtttttga tatcgacccg ttcgtgttgg ccttgaccac
     3481 cccagaggcc tgggaggctg agtacggatc cagttgcaac ttgctttgca cctgcgactg
     3541 caacagcgac ggctccgtga acgtgttcga catcgacccg ttcgttagct tcctcacctc
     3601 cggcagctac tagcagaacg ggctctcgac ttcgattaac cctcgcgggt agccactgct
     3661 tgaaccgcta ggttcaagca gtggctaccg atctaagtgc cgagtccggc tcaacctgaa
     3721 cgcgaacagc cctagtttgc cgctgaagcc gccttgctgg gcgtgcgact cggccagcgc
     3781 ccaagcacaa ctggcacata tcgctcaatc agccagacga tcggcaggca cacagccact
     3841 gatagcagcg tcaggcccac acacgtcgcc gtcagtatca gaacgctatc cttgggaaga
     3901 agcggcgcca cccgctgcgc cagcggtagg ttgaccagcc cgtgcagaat tccgttcagg
     3961 cacatcaaac ccagggtgat ctgcccgaat ctcgccagaa tcccgctaaa cgacagcaga
     4021 cttgacaagc ccaccatgaa caagatgccg gcaaaggccg tcaagaagaa ccacccgacg
     4081 tttccatacg ctccgtgcgc cacgtatgcg accgtatgca ccttgttata gtttaaggtc
     4141 accaaggtaa cgaccaagcc gaccgctcct gccgcaacca gtggcagccg acgcgaggcg
     4201 agccccatcg cggtaacccg ttggaacaaa atgccgccct gataaaacac aatcgcggcc
     4261 accaccgagc tgaactgcca aaaatcgatc ttgagcagcc ccatgtcgaa cggcagagaa
     4321 ccccagatgt cagcgatctg gctgcgataa cacgtaaaca cgtgagttat tccggcgaaa
     4381 accgccatcg acactaccag ccagagcgtg cggcggaacg gcttgcgcag cgcgaattga
     4441 atcagctcca ccacgaacag gcacaccaag aaccacacca gcacgttgaa aaacggccgc
     4501 ccgcagaggg tctcccatcc gaagcgtacg gccttcgcct tgaatatggt cgcgagctgc
     4561 gtggcgggat tggaacgcgt ccaaaattca atgaacacca ccaaaaccat gctgatgaaa
     4621 ttaaacaccc ataccggcgt caaccgcgaa agcgtttgcc gcttcagaaa agtctccgcc
     4681 gtgatcgaac gctccttgta caccaggccc gagaggaaaa agaacaacgg catgtggaag
     4741 cagtaaatcc agcgccattg ttccagcgcc gcctgccgcc attccacgag gtccccctgg
     4801 cgcacggagc ggaaaaagca ctcgatcagg tgtccataaa acaccagcaa aatgcctaga
     4861 gctttggcta catcgacgta ggcaatgcgg gtcgcaggtg cgcggctggg cagggccgtg
     4921 gcggccggcg cgccgcttgg gtgggcagtc atggtttgtc cttactatgg ctccagccgc
     4981 gggctgaccc gggctgcaat gcgtgctgat tcgttactgc ggccgccggg gcacacacgg
     5041 tttcgcgcgc ccccaggtgg gcgcgcgtga tcgccaagag ccaagccagg ctgatggtgg
     5101 tcaacaaccc tagcagggcc gccagtaacg gcatccatgc ctgtgaaccc gacggacggc
     5161 gtggacctcc gtgtcgtttc gccataattc ctcatcctct tcctcgcagc cccgcggagg
     5221 ctcgcaggct gcttcaatgg tacaagcgcg ttatcaagac cgcgctcagt accgccaggg
     5281 cgccgaggcc caatatcgcg gctaccagca tgatcaacag gaagcgccgc gtttgctccc
     5341 ggaccagcag ggtgagcgca tggcgctgct cgcgctcggc tgcgttcagc ctctcaagcg
     5401 tctgcacctg ggccgcaccg gccgcctgaa gcgcctcgac cgcctgtccc agtttctgca
     5461 gcgagtgcat gagctgcgtg tcggtttcct gggccacatc cattcgccgc accattgagc
     5521 gaaccgcgtc tgcttccgcc tgcagcagtg gcggcaagag gctcagcgtt tcagacatgg
     5581 cggccgtatg gcggctggcc aggctcacgt tatcggccat tgcacgcacg tgctcctgct
     5641 gccggtggcc gtcgtccgca agctgttgaa gtatgccgct gatgcgctcc gtctgctgct
     5701 cgactcgggc cgcacgctcg tcttgctgcc gatagtgctc gcgcagtgat tcgaccatct
     5761 ccgccatgcg ctgataagtg gccagttgcg cggcgcgcgt atcgctgcgg cgaaaacgcg
     5821 ccaggaaccc gccggtcgga gaaaggccgg gcgcggaaga aaggccgggc gcggaattcg
     5881 acccggccat ctcatgcgcc gcgcgcgcgg catcagcgct cattggtggg gcggcgtcag
     5941 gctgaccgtc agatgcgctt ttaccaaaaa ggctgccgat acggcgccag aaggacgtcg
     6001 cctgcgacat cggcgtacct ccagggctgg ttgaaccctc acttgccggg gcattatacc
     6061 gagtcgcccg gcaatatcga gcagaggcgg gacgtgccgc agcagatccg ggggcgtcct
     6121 tctgtcatct gcggtcttcg cgctcttggc gtctcggcgg ttgccttgtg tcgccttcca
     6181 gcacgccgcg cttttcgtgt agtatgtccc gcgcgacgcc gccctggttg aacggatgag
     6241 caccattacc gaccgaagaa ctcgcctgac cgagcgctcc tcccgtcggc aggttgttac
     6301 cgtgccttgg tggcaacgcg acatggtgct cgcggccgcg gtgttcgtgc tcgcagtggc
     6361 ggtattcgcc aactcgtacg tgaacgacca cgtttacgat gacatcggca ttatcgcgac
     6421 aaacacgcgc ctggactggc cgtgggacct gaaagccctc ttcgccaccg actattgggg
     6481 cgaaaaactc actaactcca agttgtaccg gcccttgacc attctgaccc tggccgtcga
     6541 ttgtgccctg ttcggcaagg acccgcccag agggatccac gccaataaca tcctcacgaa
     6601 cgcggtcatt gccgtgctcg tttacgcgct catccgacga ctgtggcagc ggcgcgacct
     6661 ggcgttcgtg acggccgcct tgtatgccat tcacccggtg cacactgaag tggtcgccaa
     6721 cggcgccgga cggtcagagc tgtacatggt gctgaccctg ctggccgcgc tgcacctgca
     6781 cctgacctac gcgcggcgca ggttcgagcg cgacgaaaaa acctctcgca cgcaggctgg
     6841 ccaaccaaag ccacgcaccg cgccgcagcc gcgcatctat ccatacctgg ctggcgcggc
     6901 ggtgctttat tttgtggcgc tgctcttcaa ggaatcggcc gtcgttctac cggcgctggc
     6961 gctgctgatc gacttactgg ttgtacggcg cggtcgccgg cccgcctggc gctactggct
     7021 cgcgtacctg gcctacttgc ccggcctcgt catctacaca accattcgac tggccgtcct
     7081 cggaccggcg ctgccggctg tccaggaggt gatgtcgtcg gctaccggtt ggcagcggtt
     7141 cctgttcgcc agcgaaacca tgttgcgcta tattggccag ctcattctgc ccatctggct
     7201 atgtggcgag tacgccgatt ttacgcggat tattcgcccg tcggttaccg agccgatggt
     7261 cctggcaggt ttggccgcct ggtttgtctg ggccggattg atcgcttggt tggcgcggcg
     7321 ccggcactat ctcattctga ccggcattgc ctggttcttc atcagcattt tcccggtgag
     7381 taatttgatt gtgccggtgg gaacgatccg cgccgaccgg ctattgttcc tgccttccct
     7441 ggggttcgcc atgattgccg ggtgggcact ggtgcgcctg gccggtgtgc aacgggccgt
     7501 gggcatcgtg gccacattgg gccttctggg cttctacggc gtgcgcagcg cgctgcgcaa
     7561 ccttgattgg cgcgatcaag atacattttg gaccaagacg atcagccaga atcctggcag
     7621 cgcggtcggt tggctggcaa ttggacacgg ttacaacgtg cgcaacgatt acgtccaggc
     7681 cgcggccgcc taccagcgcg cctaccagtt gcgcgatagc gccggcttct tctacgaaga
     7741 cgcccatctg gattgcgcct taatgctcga ggccctgggc gatctgaaag gcgccgccgc
     7801 tgaataccgc gcggtcctgg agcaatatcc gaagcacaaa gcggcgctca tgaacctggg
     7861 ccaaatttct catcgtgatc cggctacccg caccgaagcg ctcgagctgt tcaaacgtta
     7921 cgtcgcggaa gagcccaccg attttcgcgg ccacgccaac ctggcgcagg cttacaagta
     7981 cgcccggcag tacgccccgg cgctcgaggc gatcgagcgc gccctggcac tcgagcgcgg
     8041 cgggcccgac gagggcaagt tgctattagt caaagccgac attctccggc tcagcggcaa
     8101 cttcgaagag tctgaacgag tgcgggcctt gggtgagcaa cggcaggcgg ctcaaaaccg
     8161 tggcggctga tcaaggttgg cgccccggcc cgcgggccgc ttccttctgc gactggatag
     8221 tcttcacgat ctgcttcgcc gcttccgccg gcacgcacaa ctcgctgcac agcgtccggc
     8281 cgtccaggta gactccatag gcgaccagcg gcagcgacgt atccggcgct ggcgccgtgg
     8341 ttccggttcg cccggaccgg tctacgcgca gcaccagatc cagcgccttc gccgcatcca
     8401 acagcagcag cacttgcggg cgcggcgaca ggcgggccca cgcttgccgc acgcgcgggt
     8461 tttccgccag cggccgccct ttgccggacg tcattcgcac gagctgcgcc gccgcccgct
     8521 ctgcggaggc ggtggccgag accaggccga ccggttgagc ggcgtagtac gtggtcaggg
     8581 tgcgaccgta cagcatttcg atcgcgcgac gcgactgctc atccggggcg gcaaaggaca
     8641 cctcgcaggc aaacgcgtcg attgactcaa tccgctccgc gcgacaggtg acatccgcgc
     8701 ttgccccgcc cccgagggcg ctcagaaatt cgggcatcgt ctcgtatgcc aaccgcatgt
     8761 tactcaggac ttgctgcggt cgagacgaaa actgcaccgc gctgaatacc agcagcggtt
     8821 cggccggcct cgcgcttacc gccgtgcaac tgccgctgat gtcgttatag gctgccagca
     8881 gagcctcgta tcccggtagg aacttctctt cccccagacg ctcccgcagc gttcccgacc
     8941 caagcagcgc tgcgaccacc gccgtattca ggccttccgt gcccggctcg agttcccact
     9001 cgtgcgcaaa aacaaaaaca gtctcgtcat ccacaaggcc gcgcagcagg tcgcgatccg
     9061 tcttaatcac tttcttcagg tacgctgccg ccgcgctgtc cggcgcgaaa ctcaacgcgt
     9121 cccgaaaaaa caccccggcc gagtcgatgc gcaccgccgc cgtgtacgtg tcgacctggc
     9181 ccagcaattg ctcggcctgc tggaaaagcc agcccaccac acgcagcaga ttctcgtcgc
     9241 gctgcccggc gagcatcatg cttaactgca gcgaagtacg tcccgcgccc agcatgggcg
     9301 agatgaggcc ggtccatggc ggcacgcgaa tggcgaccac gagctgatgg tccgacaacc
     9361 acgctcgcgc acgcggcccg aaccgcgccg cgtaatcaaa gctgccgcgc agcgccgaac
     9421 gcaccgcgtc cggttcgtcc cccacgatca gcagattgcc cgcgctggcg gcataggccg
     9481 gttcgccgaa catctgcacc cgccacagct ccccatccgc cggctgcgcg cccgaagccg
     9541 tacgccactc ttcggcgtcg agtacctggc ataccagcag cgggctcagt cgggtcggct
     9601 cgagcgctat cacgagcggt cccgcgggat ccagcccgtt tatgcgctcc agcacgtcca
     9661 attcgttcag gagtttcgcc ggacccagtg tgcgcagcct ttcaaccccg atgctgccgc
     9721 caaattcggc caaaccgctg accagtttgt cgaggctggg tgcgacgatt atgagcgcgc
     9781 tttcctcggg aacgtgtgaa agtgcccggt ccagcctatc gcccgctcgc gcaatacaca
     9841 cgctggcgag tgcgaaccag aacaccaggg gccatttcat agcgccgttc ccaatccggc
     9901 cgccgcaggg ctcagccgcg cgcgtccata cctccccttt cggattaaag cataagacgg
     9961 acctttctga tgcgcgctct gccggcgcct tacaattttc gacacccgcc gcgcgccggg
    10021 gggaccacgc attagttcgg tgaaaaacgc gactccgcaa gcgtctgaaa cacgggaccc
    10081 gataaacccg gtccgcggag gcggcgcacg ccgtactgga ggcgcagccg ttgtccgccg
    10141 atgcttgtgg tgaggtctcg atatccacta gttctgcgcg ccgctgcgag aatcaagtgt
    10201 ccgacggcga gcctgacgca actgctgtct tggagtcgtc ccaaccgggt gaacagtgcg
    10261 aacccactgc gcccgagccg cacgacgcca tacccgtcgt gcccgttcct gtgccggcgc
    10321 acgtgcagca gcccaagccg cgcgcgcccg cggtcaccgc cggccccgaa cagcaacagc
    10381 aggcgcagca acagcaagcg cccagcgcta cgacgccctc acatcccagc gaaccgccgc
    10441 tctcgcacgc cgaggggcaa gaagggcccc ggacaacgct ccagcgaatt ctgcacgcgc
    10501 tccggccggg ccggcggaaa gcgtcccgcg ctcaaaacgc cgcggaaatc agcgaaccag
    10561 ccgccggcgc cagacccgct gccatagtgg tcgtgctgga tttagtgcta agcctcataa
    10621 accgcccgtt cgccttcctc tcgccgggtg cccgccacgt cgtcggtctg gtggcgattg
    10681 tgacgctggc catcgcggcc gtcacggcag cgctcgctcc actggcgcca aagaacgacg
    10741 cagttacgtt catacgccag aagcgggccg aactcgacca gccacccccg ccgcccgcgg
    10801 cgcagtctga gaagcctcag gcggaaaacg cccacggcgc agggcactaa ccggccgccg
    10861 ctcagacgcc tcgcttcaaa actgcacgct caaaaacgca cccttacagg gggccgctga
    10921 gcactagaac gaggttatag gccgcgtggc agccggccgt tatccccagc ccgcgcaaca
    10981 agaagaccac cgccaagtac gcgcccgccg ccgtaagctg caggaactga gtcagctcaa
    11041 acggctcgat gccgatgggc tgaaaatggc acgccgcgaa gatggctgcc gatactacga
    11101 ccgccgtgca cggcccggcc gcattgcgga tccgcaaccc gttctcgaaa agccacagca
    11161 cggcgcaaac cacgcccagc cggaacacca gttcttcgta cagaccggcg cccaatgcca
    11221 ttaccatctg ctgccgcagg tcggccagat tcgcggcgga gctgggcgcg gctagcagga
    11281 gccgccccag cacaaagagc ggcacgatga gcacgacgct ttcacaggcc atcagtggca
    11341 agacccatcc gcgcacgcgc cagccttgtc ccgacaggag gtgccacacc agcagcgtcg
    11401 ccagcagggc cagcgccggc aacagcgtgc cgctggcccc gaaccagccc agcaagttct
    11461 ggatcaggct gtgcgccacc aactgccggc ccgggcagct taccggccgc agcagcaacg
    11521 ctccgaattc gtacgcggcc accagcggga acaagaacag gaggcaatat agcgggcgtt
    11581 gcgcgtcctc cacgtaggca tgccaggccg acgtgggctt gcggttggcg ggccgcgatt
    11641 tgacgcgcga ggcaggcatc atggcggcat tatgcagctt ttcgcgccgc tgcacaggac
    11701 ctcaaggggc tgctcgcgac cggacctcat cctgcggcgc aaggtagcgc cctatcagcc
    11761 cctccatgta cgccgcaaac cgcgccgcga actcgccgtc cagcggccca aagatgtttt
    11821 cgaactgctc caaggcgccg cctgccggcg ctttgcccgc gatcgtcacg gcccgcacgt
    11881 acgcgccgaa gttcgtgcgc tgccggacgt acaggtactg tgtcagcgcc cacgccagcg
    11941 aatagtccgc cccgccgtgc cacgatgccg tcgccacgag gcgtcgcagc gcagccagca
    12001 agtcgtcgcc cttctggagc tgctgaaact cgcgcagccg gtagcggttt accgatagtc
    12061 ccaggctcgc accagtttcc acgaagggca gctcgaacat ctccgcgagc ccctcgacca
    12121 gccaagtcgg cgaattcgag tcgggcggaa tcagcccgat gttggcctgg accatgtgcg
    12181 ccgcctcgtg ctgcaagatg cgctgattca ttccggccag actttgctcg atttgcgccg
    12241 ccaggcgcct tcgtcggcct tcgtcctgcc tgtgcagctt gtccagttcc tgccgcaggc
    12301 cagtaacctc cgggtgcgtg ttgaaatcga agaacacgca gcgggcctcg gcggggctga
    12361 agtagcccaa aatctgctca tccagcccgt cggcgcgcag ccgggagtga aagccggcgt
    12421 agtcacgtag cagaagcacg gccaatttgc cggcaggggg cttaacatcc aggcggagct
    12481 cacgcgccaa ccgcacattc gctcggtaca cagcctccag gcgtggcgcc agggcccggg
    12541 cctgagcttc gctgtcggca tggatgacga agtgctcgga gcaaaacgag gcgccgggtt
    12601 ccggcgcaac gctgctcgct tgccaagcgg atgactggcc cgccgtcagg ccgagcatca
    12661 acaacaagcc ggcgagccgg ctcaccggcc gcatgccttt gtcaagcctg cccccgcaaa
    12721 ctgctccaga cgccggactt atggataatc cttcggcggc agcaaagacc gtttgcgcgg
    12781 cagctccatt atgtacttcc agaaatcatc gacccatttc tggtcgatgg tgccgaagca
    12841 atcctggaac tcgcgctcgc gctgtgaata gctgactgct tcacctggat cgcggctact
    12901 gataatgtga tagtactcga ttaatcccgg gcgctttacc tcccacagat aatagaccag
    12961 ggcccagccc atgggatagt accagccggt ttgcagccag atctcgttgc gcaccacgaa
    13021 cagcttgaag aaatccggcg accacttctg ggcgaagttc gggttggtct ggaactgctc
    13081 cacgcgctca taattgatca cgcccaagct agcgccctcg gtggttgggg gaacttcgaa
    13141 catcatcgtg gtcccctcca ccagccatct gggaatactg tcgaagctct ccatatcgag
    13201 gaacacagct tgcggaaaca ggccgagatt gaaatcgatg tgatggccca actcgtgttg
    13261 cgtcactgat agggtttgat actcagccca ttgccgcgcc atgttctcct cgcgcgccct
    13321 taccgacgcg ggagcattct tgaggctctg gagccgcatc ttgatcggcc gccaggtgag
    13381 catgtcgaaa aagtgcgaac ggttcgagtc tggcgagaag aaacccaatg ttccatcatc
    13441 cggctgcccg ccgatgttga cttggagcgt cttgaattct tcgtgatcag caaagtaata
    13501 cagctccagc ttgtgctcgg gcattcgcag cggcaagttc aacattttca taaatctgac
    13561 gcgccaacgc caaattgcct ccaggcgcgt cgcgatctcc gctgctttct gcttagtcga
    13621 agtgtacaca actacgacat gcttcgtggc atagatattc tccgtcccag cgatacgcat
    13681 catttccccc accgactcct catccagcgg cagctcaatg tcgtccggat cctcgtccgg
    13741 cggcattaaa cccgtcccct ccaacagctt ctctatttcg aggtccttca gtccgctgcc
    13801 gtgtttgctc tcttcctgcg cttcacccgg cgactgcgtt tcctcgaccg gcaggatggc
    13861 cttgaccctg gtcttcggaa tcgtgaccgt gatgctcccg accttgacga ggtaagcctc
    13921 gggcgtttcc tcaacgtcgc ccgtcaccca cttcccgtcc gtttgctgaa tcctcatctt
    13981 gcccaggttt tccgcctgaa cttctggcaa aacgcaagcc gccagcagca gcgctatgag
    14041 caagccgaat ccccaccttc tggcatgttt catggctgtt acccttccgg tgctgcgcac
    14101 ccaaatcacc cttccaagtc tatcacggcc cggctgtccg gagcaaccgc tcggcgtgtc
    14161 ggaaagcgcg cacgccgggg ttgcgctctt gtcggcgccg gctcaacgaa tagacttcac
    14221 gtgcctcgag gagtgcccca tggaccacgt tcgcggtggc gagaccgcgt tcatctcgcg
    14281 cctccgggcc gaattgcccg cccccctccg tctggcgcgc cctctcggcc gcgatcaccg
    14341 cccgctcgac ttcctcgacg acatggcggc cgtggccggg cccggctctg atctgctctg
    14401 gaccgccgac atgctcatgg acggcgtcga tttcgactcc cgccggcacg cctgggccct
    14461 gatcggtcgc aaagccatgg ccgtcaacct gagcgactgc gccgccatgg gggtacgccc
    14521 ggtttcggcg ctctgcgcgg tggcccttaa caaccagctt tcgatggacg acgccgtggc
    14581 cctggtgcga aatgctcata atatgggact cgccttcaat tgcccgatcg tcggcggcga
    14641 cacgaatagc tgggacgcac ccaccgtcat cagcatcacc atcgccggcc aacccgagac
    14701 cggtcagcat ccggtccgcc gcgacggcgc acgccccggc gacttgatct gcgttaccgg
    14761 accattgggc ggctccatcc ttggccgcca tctgacgttc gagccgcgcg tcgaactcgc
    14821 cctgcagatc gttcgctcgc tcgcccccca cgccatgatc gacatcagcg acggcttggc
    14881 gcttgacctg caccggctgc tggaagcctc ccaatgcgga gcaatcctgg acgcggccgc
    14941 aatcgacggg ctcatacatg acgatgccca cgcgctcgcc gcgcaagatg ggatttcgcc
    15001 gcgccagcat gccctcttcg acggcgagga cttcgaactg attgtcattc tcgaccccgc
    15061 cgcgcccgcc gccgaactgg cgcatctgcg cctgtcgccc atcggccgaa tcgtgcccgg
    15121 cgccggcgtg ctgctgcgcg aagccgacgg tcgcgaactc accctcgaac gccgcggctg
    15181 ggagcacttc aagtgacgcc gaccatcgac tttagctcag catcgcccga ggagactgtg
    15241 cgcctggggg aggccatcgc ggccgccgtg cagccgggcg acgtgctcgc gttgtacggt
    15301 cccttgggcg cgggaaagac gcaattggtt aaaggcctcg ccgccggcct gggagtaccg
    15361 ccggacgaac cgatcgtgag cccaaccttc gtgctggtcc gtgaatacgc cggtcgactg
    15421 cggctgtacc atctcgacgc gtaccgcctt tccggttcag acgaactggc cggcctcggt
    15481 tgggaagaaa tgctggatga tccgcacgcc gtggtagtgc tggaatgggc cgatcgtgtg
    15541 gcagcggcca ttccgccggc agcgtggcgt atcgacctcg agcacgccgg cccagaggcg
    15601 cgcacgataa gaattacctg ccccgacgcg cggcgcgccg caccgctggc tcggctgctt
    15661 gcgcccagtc gtgggccgta gcatcccgag ctgcccgagc tgccgggcgc tgcgggctcg
    15721 gattcggcgc ggctgattcg cggcgcccct cgctcgcgct gaagctcagt cgcggttcgg
    15781 caaacggcgc gacttgtttc gattcgcgcg gcgtggtcta ctgtcacaat tcggccgtgc
    15841 gacgattggt cgccgtgcga agaccggtcg cggcttgttg ggagagtttg atgcggattt
    15901 tgtcgggaat tcagccttcg ggcaccctgc acctgggcaa ctacctgggc gccatgaagc
    15961 agcacctcga gctgcaaacg cagcacgagt gtttctattt catcgccaac ctgcactcgc
    16021 tgacaacgat ccatgacgcc gagcggctgc ggaagctcac gctcgacgtg gccctcgatt
    16081 atctgacgct cggcctggat ccgcagcgcg tgacgttctt ccgccaaagc gacgtgcccg
    16141 aggtcaccga gctggcgtgg attctctcaa cggtcaccgg aaaaggcctg ctcgaacggg
    16201 cgcactcata caaggacaag atggccaagg gcctcccggt caacatgggc ctgttctcgt
    16261 acccgctgct gatggcggcc gacatcctga tctatctcag caacgaagtg ccggtcgggc
    16321 gcgaccaggt gcagcacatc gagatgaccc aggacatggc cgagtacttc aacaacacgt
    16381 acaaaccgat cttcatccgc ccggagccgc ggttgaacga ggcgccggtg gtgcccggca
    16441 ttgacggcca gaagatgagc aagagttacg gcaacatcat tgagatcttc ggcgaaccca
    16501 aggccatgcg caagaagatc atgagcatca agaccgacag cacgccggtc gaggagccca
    16561 agcaccccga aacggataac gtgttcgcct tgctcaagct cctggcgagc ccggaagaaa
    16621 ccgcggaatg ggagcgccgc taccgcgccg gcggcatggg ctacggcgag gccaagcagc
    16681 ggctgtttga actctacgag gcgaaattcg ggccgctgcg cgaggtccgc gccgattggg
    16741 cgaagcgccc ggacgaggtt gaggacattc tgagcaccgg cggccggcgg gcgcgggcca
    16801 tagcgcgcga cgtaatggcc caggtccgcg acgcgtgcgg catcgtcgtt tctcgggaat
    16861 gaaccgaggc aacctccaag acgccgttaa ccgccaagac gccaagagcg ccaagaggat
    16921 ttgaatggtt ctgcgagaaa cagtgcggcg tcgtgaacct acaagcgcgg ttgcctttta
    16981 attatgtcag aaggataccg ggtacaactg gaagtgttta ccgggccgct ggatctgctg
    17041 ctttacctga tccggcggga cgagttggac attcaggaca ttcccatcgc ccggctaacc
    17101 gagcaatacc tggcctacgt acacgttctg gagcagattg acccgaacgc cgctggcgaa
    17161 tttctcgtga tggccagcac cttggtggag ctgaagtcac gcgccctgct gccgacgccg
    17221 ccgctggagc cgatggagga cgacgcgtcc gatccgcgca ccacgctggt acgccagcta
    17281 ctggaataca agcgcttcaa ggatgcggcc gaagagctgc ggcgggcggg cgaggagcgg
    17341 gcgcagcggt ttccgcgcag cccggccaac ttgcccaaag agctgcaagg catcgagctg
    17401 gaagaggccc aggtctggga cctgctggaa gccttcgggc gcgtgatgac ggccatcggg
    17461 cgcggtccga cgcagcatca ggtgcaatac gacgaaacgc cgatcgaact ctaccaggcc
    17521 gaaatcgtcg cgctgctcgg agaaggagtg gccacgtttc agtcgctctt cgacggccag
    17581 tccactcggg gcgaagtcat cgggcgcttc ctggcgattc tggaactggt ccgcagcggc
    17641 cgcgtgcggg ccgagcagga tcgcaacttc ggcacgatat acctgttcct ggcagacccg
    17701 cgcgaaccgg aaggggaggt tgaaccgggg ccggaacttg cagagccggc gcccgatgcg
    17761 gcgccgtccg ccggcgaagc ggggctgtct gccaacgacg cgcgtccgtc cgacggcgaa
    17821 gcggatctga ccgccagcga agcccggctg tccgccagcg aagccgggct gactaccagc
    17881 ggcgcgcctc cgtccgccag ccaggcggcg cctaggacta gcgaagcgcg tctggaggcc
    17941 actgcgcacg aggctgattc cgccgagtct gattctgccg acgctgacgt gcctggcctg
    18001 gagccggcgg aagaggcagc ctgcgcggcc gagtcgcctg agacgccgcc cgcgccggaa
    18061 cagtctttgg ccgttagcga ccgcatgtcg ccggccgcag agtcttgcga aacctgttct
    18121 acggagcatt ccaatgagcc agaccagtca acgcttgaag gaccttaaca tcaccttgcc
    18181 gcccgtcagc aagcccgtgg gcgcttacgt gcccgcgctg cggcacggcg acttgctgat
    18241 gctcagcggc cagattccgc tgcgcgaggg caaagtggcg tttacggaca aggtcggcgg
    18301 gccggcggga cgcacactgg aggacgccca ggccgcggcc cggctttgca cgctgaacgc
    18361 gatcgcagtc gcggccgacg cggccggtgg cgttgaccgg atcaagtcgg tgctgaagct
    18421 cgtggtctac gtggcctcca acccgggctt tacggaccag cacaaagtgg ccaacggcgc
    18481 cagcgatctg ctggtcgagg ttttcggcga ggccggcaag cacgcccggg ccgcggtcgg
    18541 cgtagccgag ttgcccctga attccacggt cgaggtggac gtaacgttcg tagtttcgta
    18601 agcgcccgag gtttcgggag cagagccggg gcgtgccgcc ccggaatgtc gggacagaac
    18661 attcaaaggg atcgagggat cgagagttgt aaagagcaga gctggggcgt gtcgccccgg
    18721 agtaacgcca caggcctcgt ccgtccgccg tacgggcgga aacagaagtt attcgttgtc
    18781 taccagggac tacgtccctg gcaacactcg tcggccctcc gggtggtagg ataattaggg
    18841 acaggcacag tcctgacctg tgggccaatt ccggcccgac ggctacaccc gtccccgttt
    18901 cacaccgtcc cgttcacacc ctgcgtgaag gcactgggca tgcgccgcct tcgtccgccc
    18961 tccgggcgga aacagaagtt gttcattgcc caccagggac tgcgtccctg gcaacactcg
    19021 tccgccctcc gggcggtcca cgttgccgtc agcgtaggtc ttccggttga gcagggcggt
    19081 ggccctgatc gatggacacg cacccgtggg aacgggggaa tgtgggaatg tggccgcggc
    19141 ggcgggtgtg gaacggggac gcgggtgtgg aacggggacg gggctagttg atggcgtgat
    19201 gcttcggtcg tccctacggg actcagcggg ggcgggccgt tcgcggttcc caggatttca
    19261 tcctgggcta cggtcatggc gtgcctacgg cactcagtcg tgttggcgcg tgctgcgcga
    19321 tcgcgagttg gccgcgtcaa ggcaaacgga ccacgcatcg acggcgcatc aggcggtggg
    19381 ggacgcgggt gtggaacggg gtggggggaa cggggacagg ggtcgcaacg tgttgaacgg
    19441 tttcggcggc gccgcgggag tcgccctacc gcgaacgtcg cgtgatgggg gtattcacag
    19501 cagccggggt tgcggaaagg attccgcggg agggcgtgcg agacggattc tcgcacgagc
    19561 gtacgacgtc ggccacgggg ggccgacgcc tacggattgc gggggaattg gttgccctcg
    19621 ctggcgcttc gggctcggat ctggggggcg atcacggggt ggacgactgc gcgggtggga
    19681 ggttgttggc ggcaatccgg ggcggcacgc gccggctctg ctctgcgatg cgaggttact
    19741 gccgatggca tgcccgcgcc cgcaacgcgg gcttgggcat ggcaccccgc gcgagcggcg
    19801 gatgagagat tatcggcggc attccggggc gacacgcccc ggctctgctc tgttactgcc
    19861 gatggcggct tcctggcaag cgctggctct cgcctccgcc gtgcccgccg gtacaatttt
    19921 tttcgagccg aggaggacgt cttgtgagcc gcctatacgc tcaggtcgct gtcaccccct
    19981 cagcgccggc gcggcgcgcg cacctgctct gcgcaaccgt tcttctgtcc gtaaccgtcc
    20041 ttctgtccgc catgctcacc tcctgttcgc gttcgaacga acaagagccc ccggcccggt
    20101 cctcgccgcg cgagaccatc gcccggctca tcgccgcacg tgaacagcgg cagtaccagg
    20161 tgatgcgcga gctgatcgtg cccgagcacg cgcgtgacgt gatcgcaacg ctcgcagcgg
    20221 tcgatgattt cctctcggtc aacgaccagc tttgtgactt gatccgctcc gagatcggcc
    20281 tgggtaccgc tgacgcaatt gaccaagggc aactcgcgta tcatctggac gtgttcagcc
    20341 gcaacgtgaa ggtactggac gagtccatcg cgggccacga cgcgcgggtg gcgttcctgg
    20401 tagacgagcg gctgccggcc cggcatgccc agcttcggct gctggatgga acgtggcgct
    20461 atgatcccgg ggcgggcgag tttgcgaagc tcagccaggc atttgagcgc atggcccacg
    20521 gtctgcgcca agtcctggag ggcctgcgcc gcgggcaact gtccgccgcg cgttt
//
LOCUS       DAOSYS010000036        20543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2136_length_20543_cov_5.688696, whole genome shotgun sequence.
ACCESSION   DAOSYS010000036 DAOSYS010000000
VERSION     DAOSYS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20543
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2136_length_20543_cov_5.688696"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLU99_03725"
     CDS             complement(<1..165)
                     /locus_tag="PLU99_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOO16196.1"
                     /translation="MNAVAIIPARYGSTRFPGKPLARQTGKYLIQHVYERAAAARLVQ
                     RCLVATDDVRI"
     gene            688..2646
                     /locus_tag="PLU99_03730"
     CDS             688..2646
                     /locus_tag="PLU99_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOO16197.1"
                     /translation="MLWRASASLWLAASLLAAAAGAEQVRYDDYKLVRVVISSLDQIE
                     QVHGIGGMLMSDNEGIGAIDYLFPPAAMADLAALNLPYQVLNDNVQKAIDAERASLER
                     WRDVGPRDPAWFADFKNYDQILAKLNQMQADRPDLCTVFSIGDSFEGRPTWAIRITAP
                     GSEKPMVVFDATHHAREWISTMTVMWIADRLVYEYDTNPTILDILDTVEVVVIPVVNP
                     DGYVYTWTTERYWRKNRQTPPPGSYCYGVDLNRNYDAGWGGAGASHDPCSELYCGTAA
                     FSTPETANYRDFVNANPRIVSGISYHSFGLLIMWPYGYMSALPPEPDRTLFAFCGETM
                     HDEIFAVHGVSYDWGPIYSTIYPASGGTVDWCYDAHGIFEFTIELRGYDFVIPASEII
                     PTAEENFEGARFLMEWSAGPVRITFPQGLPEYISPVDPTSFTVRIEDGYQSVVPASAT
                     LYYTYDGAAFETASLVHAGGTLYQATLPPAACGTTPRFYFSAQGDGGATAYRPATAPA
                     ETFTATVALVTTVLADDFESDLGWTVINDPSLTTGAWERAVPFSPASGGAPGIDFDGS
                     GKCFVTDNRPGSFDVDGGPTRLISPVLDLTGFEDPVLRYARWINCDDELPPAQDFLNV
                     EVSDDGGATWVLVEHASGRGMWSIVSGG"
     gene            2622..2969
                     /locus_tag="PLU99_03735"
     CDS             2622..2969
                     /locus_tag="PLU99_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16198.1"
                     /translation="MVDRQWRVSDFVSLTSQFRVRFSIADNPNNSMTEAGVDAVRVYD
                     ATCTTGLPGDLNCDGAVNVFDIDPFVLALTDAAAYDAAYPTCDIELADVNDDGQINAF
                     DIDPFVLLLTGTS"
     gene            3046..>3593
                     /locus_tag="PLU99_03740"
     CDS             3046..>3593
                     /locus_tag="PLU99_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family carboxypeptidase A/B"
                     /protein_id="HOO16199.1"
                     /translation="MRWHKWWLPVLTLALVCAPLAAQETTPVRYDNYKLVRVAIKHTG
                     QIDQVHALGAQLMSEGEGLGPVDYLIPPESMPALQKLQVPYRVLNDNIQAAIDAERAS
                     MARSRAVSPLDPDWFADYKTLEQVNAKLNQMAADRPDLATVFEVGRSFEDRPIYGIRI
                     SGPGAGKPAIMFNGCHHAREWIS"
     assembly_gap    3594..3693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3694..5110
                     /locus_tag="PLU99_03745"
     CDS             <3694..5110
                     /locus_tag="PLU99_03745"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOO16200.1"
                     /translation="AMRDFTLAHPQIVATQSYHNFSQLIMSPYGHTTALPPEHEFFME
                     LNKGQHEAILAVHGVTYEYGPTCTTIYRVSGGDVDWYYVERGIFSFCIELRDTGTYQF
                     LLPADQIIPTCEENMAAALYLAEWFTTPVKFAFPDGLPTRLTPDTPENVTVKVIAVGD
                     TLDVASPRLYSRIGSGGAFTESTLIPLGGNLYQATLPPTPCGRTLEYYFSAATTAGVV
                     GVSPQDAPNTTYDTFAVPIVIVLNEPLSTNPGWTTQGQWAWGVPAGLAGDPQAGYTDG
                     TVYGYNLNGAYTNNMPEYHLTTGPIDCTGLTDTRLSFWRWLGVEKSAWDHAYVRVSND
                     GSTWTNVWENPSTTLIDTAWEYQELDISGVADNQATVYLRWTMGTTDSADVFCGWNID
                     DVLVRGLDPDGCPPVLGDLNCDGALNVFDIDPFVLALVDAAGYAAAFPTCDRMLADAN
                     GDGQVDVFDIDPFVMLLISGD"
     gene            5239..6279
                     /gene="rlmN"
                     /locus_tag="PLU99_03750"
     CDS             5239..6279
                     /gene="rlmN"
                     /locus_tag="PLU99_03750"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOO16201.1"
                     /translation="MLGVSLDELRPWFVEQGQPRYRADQVADWIFAKDADSFEQMTNL
                     GKPLRAWLAERCELYRSTLTREAASEDGTRKLLLTFADGAAVETVWIPEEARQTACLS
                     SQVGCPVGCAFCASGLEGVQRNLTAGEIVEQALRVRRLIRNAAGDPAARLTNIVMMGM
                     GEPLANYQEVVKAIRIMNAAWGLGIGARKITVSTVGLPQQIRKLAGEELQLNLALSLH
                     APDDDLRRRLVPWGKVPIAELLDACEYYFRHTGREVTLEYVLLEGVNNLPEHARRLAP
                     LARHLRANVNLLRYNPVPGLPFERPTAESAFAFQQQLRDLGVNVHLRSSRGQDIAAAC
                     GQLRRAAATTAT"
     gene            complement(6402..8933)
                     /locus_tag="PLU99_03755"
     CDS             complement(6402..8933)
                     /locus_tag="PLU99_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO16202.1"
                     /translation="MSHLSRLIAGFVVAGLGLAVVVGSTASIDPGQAIVPKSAAADRL
                     DSTHALPSPAPPDTPADASKFTPELVKQLEESAPANLVSCLVFMSEKYPYAAVRNDSR
                     QDKIATFRSVAQNSQAPVVAALAQWGNAAEVKEQFWVVNGFHLLATPDVIRWLSKRPE
                     VAAIADDAPVFLVDAGTETTAGATDTPRWNIDKVSAPDCWTAGYDGQGVVIAQTDTGV
                     DTAHPALAGRFTGYWHDSINGQSSPYDDHGHGTHAMGTTLGLDGIGVAPGATFVAVKV
                     LNAAGKGSSSQTLNGLQWVASLHATVDIKVMSASWGSPSRTDQWAWNVCQTYKSIGIL
                     PVFANGNDGPGGATVGTPADYPLVLGVGATNSSDNVAGFSSRGPAPASAPWSIETYWF
                     RPDWNFTKPDLSAPGVGIYSCWPNGQYRTLDGTSMATPHVAGAAAILSQANPNVDTTI
                     LYQMLLAGSDQPSQGHPYPNQNYGWGRLNIFQALNNGADCNGNGIPDFCDVSCGSPGA
                     FCDIAGCGGSLDCNNNGLPDECDISSGRSDDCNNNGVPDECDVDSGFATDCNRNRIPD
                     ECEVSDGRAEDCNANGIPDQCDLLPSPDTPAEDACAEAKVVGPGVTIHGTTVGATNDG
                     SATCGLSAASPDVWYSYTPTGNGFASFSLAGSAFDTVLSVHSGCPGTASNQVVCNDDY
                     YGTQSALSLFVQTGQTYWIRISGKNGATGDFQFTLVGPAGVSGGDCNSNDVPDECDIA
                     SGSSQDCNGNGVPDECDIAGGESLDADGNGVPDECEAGAMIGDLNCDGVVNSFDIDPF
                     VLAITNPSGYQAAFPGCSIMNADANADGSVNSFDIDPFVGLLTGG"
     gene            9457..9873
                     /gene="nikR"
                     /locus_tag="PLU99_03760"
     CDS             9457..9873
                     /gene="nikR"
                     /locus_tag="PLU99_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOO16203.1"
                     /translation="MPGIVRLSISLEKPLLDQLERLVKASRYTNRSEYLRDLIRQRLV
                     EQEWSDQRQEVIGTITMVYDHRSRLLSDKLVEIQHEHHRHVLAATHVHLSRRLCAEMI
                     MVRGSASSLHALVDKLRQQRGVLHAELGMSTTGKHL"
     gene            10020..10424
                     /locus_tag="PLU99_03765"
     CDS             10020..10424
                     /locus_tag="PLU99_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013470200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HOO16204.1"
                     /translation="MSCVDEIIGVHPALPRIDSDVLRGCIQACAACGQACAACADACL
                     SEPQFAKLVRCVRLNLDCADLCTAATHILLRQTQPDARVLTSVLQAVVVAARVCGMEC
                     DRHAGRHEHCRMCAMACQHCERACCELLRAGP"
     gene            complement(10492..11745)
                     /locus_tag="PLU99_03770"
     CDS             complement(10492..11745)
                     /locus_tag="PLU99_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOO16205.1"
                     /translation="MGKKRIQRTDGTWITGEVEETPSEYIVKVTRSATVTIPKNQVKA
                     VLPVEEGESPAAAEKSEHPEGASGIPDAEIEKLLEGFNIVLPASDLELEEGELPLNEE
                     SVEEMKAIIGATGIVTTKHVVVVYTGPEQNAKDIARRLETIWRWNIRFMRIINLPVRL
                     PEYKLELYYVDSREEFDALCTNVEGGPMSALGFFTPVTNRSHFYNMMSERAVKARLEH
                     ARRVGGAVRTREENLAKLWAEYQTLEVTQHELGHHIHYAIGLFPRFAFMDYISLPAKP
                     TWLVEGTTMQFEIPPSAEGGSFGAFNHERLNQFRRYYPKPSPDFLKLFMVDSQVWYSG
                     GGYFYPMGWALVYYMREKKPAGLAQYYRIIAAREAGEEISYSQREREIEECFGKINQE
                     WVDEWWDFMSKLELKRSLLPPENYP"
     gene            12082..13209
                     /locus_tag="PLU99_03775"
     CDS             12082..13209
                     /locus_tag="PLU99_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO16206.1"
                     /translation="MTEQQDFGEFELLRKLMETPGVSGREERVRDLILAETAGLWDET
                     RVDNMGNLICVKRAVTSAKRRRGAAAAGTEARAEPLRVMLACHMDEIGFYVRFIDENG
                     FLRVQNVGGFDARNLFARRVLVQGKRDLVGVLNPAGKPVHLASEDEKKKIPEMREFFV
                     DLFLTKRQVKKLVDVGDPVTLIQRTEMVGDSITGKALDNRIAVWAAISALRRAASKLR
                     YDVYFVACVQEEVGLRGAAPAAFGIDPHAGIAIDSTLCCDTPGTSKDDAVTEFGQGVG
                     IKIMDGASISHRGLFDEFVAVAQRNRIPFQREVLPRGGTDAGAVQRARAGTKVITLSI
                     PTRYVHTVTEAVHKKDARAAVDLLTAWLSTEPAKSMTNAKH"
     gene            13293..15056
                     /locus_tag="PLU99_03780"
     CDS             13293..15056
                     /locus_tag="PLU99_03780"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOO16207.1"
                     /translation="MNKSDPVPALVVASVLIGLACPAGVLIASETMPTCQTAADAVYV
                     NANIYTMNADRPRAEALAVAGQRLVAVGSNAEILKTVGPATRVHNLGGQMVLPGLIDS
                     HCHFAALGSYSLGHVDLSGARSFDEVIEIVAARAAKAEKGTWILGGRWDHENWRDRRL
                     PAHAALSAATPDNPVWLRRVDVHAGIANAAALKLAGITSATPEPPGGDIVLDEQGEPT
                     GLLVDKATELIENLIPAATDSAAMILKAQELCLAVGLTGVHDAGIQPPEVDIYRKLAE
                     QGALKIRVYAMIAGAAAPQYFREHGLYTSERLTVRSAKFYADGALGSRGAWLLAPYSD
                     RPTDEEGRPYVGLNVVSPEELRRAAEDALRQGYQVCTHAIGDRANREVLNAYEVIRQS
                     GNSSSQGASRDHRFRIEHAQILAPQDLPRFAELGVIAAMQPTHCTSDMRWIDERIGSQ
                     RSAGAYAWASLLRSGVRLAGGSDFPVESHNPFLGLYAACTRQNAAGEPAGGWHASERL
                     TREEALRAFTLDAAYAAFEEGQKGSLEPGKLADFIVIDRDIMTCEPREIVETRVLQTV
                     IGGEAVYSDADFAAEGPANHR"
     gene            complement(15064..16296)
                     /locus_tag="PLU99_03785"
     CDS             complement(15064..16296)
                     /locus_tag="PLU99_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOO16208.1"
                     /translation="MSRFAVLAFTLLFSSMVLAQPAGRVGEPDDEALRQRAAELSRRG
                     AQFLLAAQEQDGGWTSQGGPGITCLALKALIQEPTVGPNHDSVRRGVEFVRRFIREDG
                     GVYGAEGLLKNYESSVALSMFAALPAESEPEAPATAPKSAPEAPSATTGPSRGDTPRL
                     RSEIAALQKFLIENQWDEAEGKSIDDPWYGGSGYGRSKRPDLSNTQMMLDALRDSGLP
                     PDDPTYKKALIFVQRCQMLGETNDQPFAKGSTQGGFIYTAAHNGESKAGTVEINGRQE
                     LRCYGSMTYAGFKSMLYAGLTRDDPRVRAALDWIRNYWTLDYNPNMPQAQSAEGLYYY
                     YHTFARALATFGEPVITDRSGRQHNWREELVLKLAELQRPDGSWINEADRWMEGSPAL
                     TTAYSMLALQAAFAPPAR"
     gene            complement(16454..19306)
                     /locus_tag="PLU99_03790"
     CDS             complement(16454..19306)
                     /locus_tag="PLU99_03790"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOO16209.1"
                     /translation="MTASLHPRSSTPPDDLPDAVNVAFVERLYADFARDPGSVPPQWA
                     EYFRRLGANGTVSPPRLGPSFRPSTIFNPPAPDGRTANGRAAPSAEQLQDRVDQLVRA
                     YRVRGHLLAQLDPLGQPRPGHERLEPSFYEFTEADLDRPVSSRTIAGPPVRTLRDVIS
                     LLRDTYCRSIGVQFMHIDDADVREWLQQRMEGTRNRLRLSRDEQLRILTRLTDAVMFE
                     EFIHKKFIGAKRFSLEGAESLIPLLDLAIAHAAGQGVEEIVIGMAHRGRLNVLANIIG
                     KSPRLIFREFEDNESRRQPAGDVKYHLGYHGDWSSDGRTVHLALAFNPSHLEFVNPVV
                     AGRCRAKQDRLADTARERCMAIQIHGDAAFAGEGIVQETLNLSGLEAYRTGGTLHVIV
                     NNQLGFTTEPEQARSTPYASDVAKMLQSPIFHVNGEDPEAVATVVRLSMDFRRQFKRD
                     VVIDMYCYRRHGHNEGDEPAFTQPVMYRTIQERKPVRDGYLEHLLKLGEVTQAEADKI
                     ADDRRKRLEEELAGARSGTTGVEPRRPSSLGELWSNYRGGPDSSVPEVDTGVPRQRLV
                     ELLNRIATVPPDFHPLKKIERLLILRKNMAAGEHPLDWATAEALAFATLATEGVRVRL
                     SGQDSERGTFTQRHAVLHDLETGAAYAPLAHLERGQAPVEIWNSPLSEAAVLGFEHGY
                     SLAYPDGLVLWEAQFGDFVNAAQVIVDQFIASAEQKWHSMNGLVLLLPHGFEGQGPEH
                     SSARPERFLTMCAEDNIQVVVPTTPAQYFHVLRRQVLRPWRKPLIVMTPKSLLRHALA
                     VSSLDELAGGRFRRILPDDVRRDRPPRRVLLCSGKIYYELWQERKNRQAERDIAIVRF
                     EQLYPLSEEAVTQALAQYPDGTSLLWVQEEPENMGAWRYLRCSPLSRAFERFDFRGIY
                     RPASPSPATGSHTLHKIEQRKLLDAALSISNGSPAS"
     gene            19538..20290
                     /gene="gpmA"
                     /locus_tag="PLU99_03795"
     CDS             19538..20290
                     /gene="gpmA"
                     /locus_tag="PLU99_03795"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOO16210.1"
                     /translation="MYRLVLLRHGESEWNKENRFTGWTDVGLSEKGVAEAHEAAQLLM
                     QGGYTFDVAYTSVLKRAIKTLWIVLEDMDLMWIPVHRSWRLNERHYGALQGLNKAETA
                     AKHGPDQVKIWRRSYDIPPPPLSPDDPRNPAKDPRYAQLRPEEIPLTEALKNTVERVL
                     PYWHATLAPAVRNGQRLLIASHGNSLRALVKYLDNVSDADIVELNIPTGIPLVYELDA
                     ELKPLRHFYLGDPAKVQAAIAGVAAQATHRPK"
BASE COUNT         3800 a         6385 c         6455 g         3803 t
ORIGIN      
        1 gatgcgcacg tcatccgtgg cgacgaggca acgttgcacc aggcgggcgg ccgcagcacg
       61 ctcgtacaca tgctgaatca ggtatttacc ggtctgtcgg gcgagaggct ttccggggaa
      121 gcgggtggag ccgtagcgag ccgggatgat cgcgacggcg ttcatgcggc aaccatggaa
      181 cagccaagtg tatccatggg cccggatagt aacctgttcg ggcggtgatt tcaaatgcgt
      241 ttgcccgaaa ccgggagtgg gcgttggagg agaaatccgg actgagaccc gccgaaaaga
      301 agaggcgcac cggcccacgg ccgcttgcca agacggtctt ataatgctcg acaaggccaa
      361 cccgccaatt ttggctcgga aaattccccc ttccggcggt acactgcacg gaacgaagct
      421 ttcgctgttg cggttgcggc ggagacccct cgaaggccgg gcgctagctt gctcgccgcc
      481 gaagcgcagc aacgcgcgtt gcgccgccgc acgtggaagc cgcgttgccg agcgcgctgc
      541 ggtcgcagcc aatccaggcg gccgtacgcg ccaggcgtca taaggttgca ccgggccggc
      601 gccgttgcac ggcaagcgcg gccgcttcgg tattgtgatc gcgcaaaggc actgacaatc
      661 atacctgttg aaggaggcga gagatcaatg ttgtggcgcg caagtgcaag cctgtggctg
      721 gcggcgagcc tgctcgcggc cgctgcaggg gctgagcaag ttcgatacga tgactacaag
      781 ctggtccgcg tagtcatcag ttccctggac cagattgaac aagttcacgg catcggggga
      841 atgctcatga gcgacaatga gggcattggc gctatagact accttttccc tcctgccgcg
      901 atggctgatc tggcagcgct gaatctgcca taccaggtgt tgaatgataa tgtccagaag
      961 gccatcgacg ccgaacgggc gtcgttggaa agatggcgcg acgtggggcc gcgcgatccc
     1021 gcctggtttg ccgatttcaa gaactacgac cagattctgg ccaaactgaa ccagatgcag
     1081 gccgatcggc cggacctgtg tacagtcttc agcatcggcg actcgtttga aggccggccg
     1141 acctgggcga ttcgcatcac cgcgccgggg agtgaaaagc cgatggtcgt attcgacgcg
     1201 acgcatcacg cccgcgagtg gatcagcacg atgacggtca tgtggatcgc cgaccggctg
     1261 gtgtacgaat atgacacgaa tcccaccatc ctcgacatcc tggacacggt cgaagtggtg
     1321 gtcattccgg tcgttaaccc ggacgggtac gtctatacct ggaccaccga gcgctactgg
     1381 cgcaagaacc ggcagactcc gcctccggga tcgtactgct acggcgttga tctcaatcgc
     1441 aattatgacg ccggctgggg cggcgccgga gccagccacg acccctgctc tgagctttac
     1501 tgcggaaccg cggcgttctc aacgccggag acggccaatt accgcgactt tgtaaacgcg
     1561 aatccacgta ttgtttccgg catcagctat cacagctttg gcttgttgat aatgtggccg
     1621 tacgggtaca tgtcggcctt accgcccgag ccggaccgca cccttttcgc attctgcggc
     1681 gagactatgc acgatgagat tttcgccgtg cacggcgtct cgtatgactg gggtcccatc
     1741 tattcgacca tttatccggc cagcggcggc accgtggatt ggtgctacga tgcgcacggc
     1801 atttttgagt tcacaatcga gctgcgcggg tatgatttcg tgataccggc cagtgagatt
     1861 attcccacgg ccgaggagaa tttcgaaggg gcccgcttct tgatggaatg gtcggcgggc
     1921 ccggtgcgga tcacgttccc gcagggtttg ccggagtaca tatccccggt tgatccgacc
     1981 agcttcacgg tcaggatcga agatggatac cagagcgttg tgcccgcgag cgccactctt
     2041 tattacactt acgatggcgc ggcgttcgag acagcttcgc tggtccacgc cggcggcacg
     2101 ctttaccagg ccactttgcc gccggccgcc tgcggcacga cgccgcgatt ctacttcagc
     2161 gcgcagggcg acggcggggc caccgcttat cgtccggcca cggcgccggc agagacgttc
     2221 accgcgacag tggcgctggt gacgacggtg ctggcggacg atttcgaaag cgatctgggg
     2281 tggacggtga tcaacgatcc cagcctgacg accggcgcgt gggagcgcgc tgtgccgttc
     2341 agccccgctt ccggcggcgc gccggggata gatttcgacg gttcgggcaa gtgcttcgtg
     2401 actgataacc ggcccggcag tttcgacgta gacggcgggc cgacgcgcct gatttcgccg
     2461 gtgctagatt tgaccggctt tgaggacccg gtgctcaggt acgcgcgctg gataaattgc
     2521 gatgacgagc tcccgccggc gcaggatttc ctgaatgtcg aggtttcaga tgacggcggc
     2581 gcgacctggg tactggtgga acacgcttcc ggcaggggaa tgtggtcgat cgtcagtggc
     2641 gggtaagcga ctttgtcagc ctgacttcgc agttccgagt gcgcttcagc atcgcggata
     2701 acccgaacaa ttcgatgacc gaggccggcg tggatgccgt ccgggtctat gacgcgactt
     2761 gcacgacggg cctgcccggc gatctcaact gcgacggcgc cgtgaatgtg ttcgacattg
     2821 acccgttcgt gttggcactc acggatgcgg ccgcgtacga cgccgcgtat ccaacgtgcg
     2881 acattgaact ggccgacgtg aacgatgatg gacagataaa cgctttcgac attgacccgt
     2941 ttgtcctctt gctgacggga acttcctgat ttgtggcata ggcgttttgc ccgtgaaagt
     3001 cacggcgcga cgccggccct acaccagaga ctggaggaag aagccatgcg gtggcacaag
     3061 tggtggttac cggtattgac tttggctctg gtatgcgcgc ccttggccgc gcaagagacg
     3121 acgcctgtac gttacgataa ctacaaactc gtacgtgtgg ccatcaaaca caccggccag
     3181 atagatcagg tgcatgcttt gggcgcccaa ctcatgagcg aaggcgaagg cctggggccc
     3241 gtggattacc tgatcccgcc ggagagcatg ccggccctgc agaagctgca agttccttat
     3301 cgggtgttga atgacaacat ccaggcggcc atcgacgccg agcgcgcgag catggcgcgg
     3361 tcccgcgccg tcagtccgct cgatccggac tggttcgccg actacaagac cctggagcaa
     3421 gtcaatgcca aattaaacca gatggcggcc gaccgcccgg atctggccac tgtctttgaa
     3481 gtcgggcggt ctttcgaaga ccggcccatc tacggcattc gcatttccgg gccgggcgcc
     3541 ggcaagccgg ccataatgtt taacggctgc caccatgccc gcgaatggat ttcnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggccatg cgcgacttta cgctggccca
     3721 tccgcagatc gtcgccacac agagctatca caacttctcg cagctcatca tgtcgccgta
     3781 tgggcacaca acagcgttgc cgccggagca tgaattcttc atggaactaa ataagggcca
     3841 acacgaagcg attttggccg tccacggcgt cacgtacgaa tatgggccga cttgtaccac
     3901 gatctaccgg gtttccggtg gcgatgttga ctggtattac gtcgagcggg gcatcttctc
     3961 cttctgtatt gagctgcgcg acaccgggac ttatcaattc ctgctgcctg ccgatcagat
     4021 catcccgacc tgcgaagaaa atatggcggc cgcactgtac ctggcagagt ggttcaccac
     4081 gccggtgaag ttcgccttcc ccgacggctt accgacgcgc ctcacgcccg atacgccgga
     4141 aaacgtgacg gtcaaggtca tcgccgtggg cgacacgctc gatgtggcct cgccgcggct
     4201 gtacagccgg atcggctccg gcggtgcctt taccgagagc acgctgattc cgctgggcgg
     4261 caacctatac caggccacgc tgccgcccac gccgtgcggc cgaacactcg aatactactt
     4321 ctcggccgcg accactgccg gtgtagtggg cgtttcccct caggacgccc cgaacacgac
     4381 ttacgacacg ttcgcggtgc caattgtcat cgtgttgaat gagccgctaa gcacgaaccc
     4441 gggctggacc acgcaagggc agtgggcctg gggtgttccg gccggcctgg ccggtgaccc
     4501 gcaggctggc tacacggacg gcacagtgta cggctacaac ctgaatggcg catatacgaa
     4561 caacatgccc gaatatcacc tgaccactgg gccgatcgac tgcacaggcc tgacggacac
     4621 gcgtttgtca ttctggcgct ggctgggcgt ggagaaatcg gcttgggatc atgcctacgt
     4681 ccgggtcagc aacgacggct cgacctggac caacgtctgg gagaacccga gtacgacgct
     4741 catcgacacg gcctgggagt accaggagtt ggatatttcc ggcgtggcgg acaatcaggc
     4801 taccgtctac ctgcgctgga cgatgggcac gactgattcg gcggacgtct tctgtggttg
     4861 gaacatcgac gacgtactgg tccgcggact cgaccctgac ggctgcccgc cggtacttgg
     4921 cgatctcaat tgcgatggtg cgctcaacgt gtttgacatc gatcccttcg ttttggcatt
     4981 ggtggatgcg gcaggctacg cggcggcctt tccaacgtgc gaccgcatgt tggcggatgc
     5041 caacggcgac ggccaggtgg atgtgttcga tattgacccg ttcgtgatgc tgctaatatc
     5101 aggagattga tgagcgataa tttgccttcg acaacgcggc gctcggacgg gaacgacagc
     5161 ggcgcgccgg ccgacgcgct gccctcgctt gcgcttcggg ctcggaacaa ttctgcgggt
     5221 cccagctccg ctttacattt gctgggagtg agcttggacg aactgcgccc ttggtttgtc
     5281 gagcaagggc agccgcgcta ccgggcggac caggttgcgg actggatttt tgccaaggac
     5341 gccgactcgt tcgagcagat gaccaatctc ggcaagccgc tgcgggcctg gctggcggag
     5401 cgttgcgagt tgtatcgctc cacactgacg cgtgaggcgg cctccgaaga cgggacgcgc
     5461 aagctgctgt tgacctttgc cgacggggcc gcggtcgaaa cggtttggat tcctgaggag
     5521 gcccggcaaa ccgcgtgttt gtcgtctcaa gttggctgtc cggtcggctg cgcgttttgc
     5581 gccagcggcc tggagggggt gcagcgcaac ctcaccgccg gcgagatcgt cgagcaagcc
     5641 ctgcgcgtgc ggcgattaat ccggaacgcg gccggcgacc ccgcggcgcg cctgacgaac
     5701 atcgtcatga tgggcatggg cgaaccgctc gcgaattatc aggaagtggt gaaggccatt
     5761 cgcatcatga acgccgcctg ggggctgggc atcggagcgc gcaagatcac cgtcagcacg
     5821 gtcggcctgc cgcagcagat ccgcaagttg gccggggaag agctgcaact caatctggcc
     5881 ctgtcgctgc acgcgcccga cgacgatttg cggcgcaggc tggtgccctg gggcaaggtc
     5941 cccatcgccg agttgctgga tgcctgcgag tactacttca ggcacaccgg ccgcgaagtc
     6001 acgctggagt acgtgctgct cgaaggcgtc aacaacctgc ctgaacacgc ccgccggctc
     6061 gctcctttgg cgcgccacct gcgcgcgaac gtgaacttgt tgcgctacaa ccccgtaccc
     6121 ggtttgccgt tcgagcggcc gacggccgag tcggccttcg cgttccagca gcagttgcgc
     6181 gacttgggcg tcaacgttca cctgcgcagc tcgcgcgggc aggacatagc cgcggcctgc
     6241 gggcaattgc ggcgggcggc ggccaccacg gcgacttgag cagcgtacgc gaatccgtgt
     6301 gcgggcgggc gctcgccctg cggggcgtta gtttgcaaaa gcagcgggcc cgtgcacacc
     6361 ggcacgggcc cgcttacttg cccggaggca cttctggcga atcagccgcc tgtgagtagt
     6421 ccgacgaaag gatctatgtc gaacgagttc acagatccgt ctgcgtttgc gtcggcgttc
     6481 atgatgctgc aacccggaaa ggccgcttga tacccagacg ggttggtaat ggccagcacg
     6541 aacggatcaa tgtcaaagga gttcacgacg ccgtcgcagt tcaaatcccc aatcattgct
     6601 ccggcctcgc actcgtccgg gacgccgttg ccgtcagcgt ccaggctttc gccgcccgcg
     6661 atatcgcact cgtccggcac gccgttcccg ttgcagtcct gactggaccc cgacgcgata
     6721 tcacactcat cgggaacatc gttgctgttg caatcgccgc cagacacgcc cgccggccct
     6781 accagcgtga actggaaatc gcccgtcgcg ccgttcttgc cggatatccg aatccagtac
     6841 gtttggccgg tttgcacaaa aaggctgagc gcggactgcg tcccgtagta atcgtcattg
     6901 cacaccacct ggttacttgc agtgccagga cagccgctgt ggaccgacag cactgtgtca
     6961 aacgccgagc ccgccaagct gaaagaggca aagccgttgc cggttggtgt ataggaatac
     7021 cagacgtccg gactggcggc tgacaagccg caagtggcgg aaccgtcgtt ggtcgcgcct
     7081 acggtcgtgc cgtggatggt cacgcccggg cccacgacct tggcttccgc acaggcatcc
     7141 tcagccggag tatcgggcga cggcagcaaa tcacattgat ccggaatccc gttcgcgttg
     7201 cagtcttcag cgcggccgtc ggacacttca cactcatccg gtatgcggtt gcggttgcag
     7261 tccgtagcga agccggaatc gacgtcgcac tcgtcgggaa cgccgttatt gttgcagtca
     7321 tcgctgcggc cggagctgat gtcgcactcg tccggcaggc cgttgttgtt gcagtccagc
     7381 gagccgccgc agccggctat gtcacagaac gcgccgggtg acccgcaact aacgtcgcag
     7441 aaatcgggaa tgccgttgcc gttgcagtcg gcgccgttgt tcagagcctg gaagatattc
     7501 aaacgccccc agccgtaatt ctggttggga tacgggtgcc cttggctcgg ttgatcgcta
     7561 ccggcgagca acatttggta gaggatggtc gtatcgacgt tggggttcgc ctggctcaaa
     7621 atcgcggccg cgccggcaac gtgcggcgtg gccatggagg tgccgtccag cgtacggtac
     7681 tggccgttcg gccagcagga gtaaatgccg acgccgggag cggacaagtc tggcttggtg
     7741 aaattccagt ccggccggaa ccaataggtc tcgatgctcc agggcgccga agcgggcgct
     7801 ggtccgcggc tggagaaacc ggcgacgttg tcggaggagt tggtggcgcc cacgcccagc
     7861 accagcgggt aatccgcggg cgtcccgacg gtcgcaccgc ccgggccatc gtttccattg
     7921 gcgaaaaccg gcaatattcc aatggattta tacgtctggc acacgttcca ggcccactga
     7981 tccgtccggg agggtgagcc ccaggatgcg gacatgacct tgatgtcaac cgtcgcgtgc
     8041 aggctggcga cccattgcag gccgttgagc gtttgagagg aagacccttt gccggcggcg
     8101 ttaagcactt tgacggcgac gaaggtggcg cccggggcga cgccaatacc gtccaggccg
     8161 agcgtggttc ccatggcgtg cgtaccatgc ccgtggtcgt cgtaggggct ggactgcccg
     8221 ttgattgaat cgtgccagta tcccgtgaag cgtccggcga gagccgggtg cgccgtgtca
     8281 acgcccgtat cggtctgggc tataacgacg ccctgcccgt cgtagccggc tgtccagcaa
     8341 tcaggagccg agactttgtc gatgttccag cgtggtgtgt cggtggcgcc ggcggtcgtt
     8401 tcggtgccgg catcaaccag gaacacgggg gcgtcgtcgg ctattgcggc gacttccggg
     8461 cgcttactaa gccagcgaat tacatcaggg gtggccagca ggtggaagcc atttacgacc
     8521 caaaactgtt ccttcacctc cgcggcgttg ccccattgag ccagggcggc caccacgggg
     8581 gcctgactgt tttgggccac tgaacggaac gtggcgattt tgtcctgtcg cgagtcgttc
     8641 cgcactgccg cgtaaggata tttctcgctc atgaacacga ggcacgaaac caggttcgct
     8701 ggcgcgctct cttcgagctg ctttaccagt tcaggcgtga atttggaggc gtcggccggc
     8761 gtgtcgggtg gagctggaga gggcagcgca tgcgtggagt caagccgatc cgcggcagcc
     8821 gattttggca caatcgcctg ccccgggtct atgcttgcag tggaaccaac aacaacggcc
     8881 aagcccaggc cggcgacgac gaagcctgct attagtcgag atagatgaga caccaatcgc
     8941 tctcctttta taggacgttc gccgcccacc atcgcaaacg ccgcacacac ccacggcaaa
     9001 caaacgctac cgaagtcatg accgcgcagc gcggcgatga acaccggact aaatatagca
     9061 ctgtactagt gccaatacaa gctcatgtgc attttttgag gttttgtgag tctcttcttg
     9121 ccactttccc agactagaaa ggaacggatc gcgcctgccg cgaatgccga ttttgcgact
     9181 gcgccgaatt ccccaacacc gcttccagcc tgttcccgac acacagtggc cacaatgtag
     9241 ccaaaatgac tcgcgccaaa acgattgccg gcatccacat ccaaggcaaa ccggcaagat
     9301 ctcttcgttc agtcgcccac tgaaagtgca ggcaaagctt tcgcccctcg cggcatctct
     9361 ctttgggtgc ggcaggcgga agaagcggcg ggcgtccgcg gggcattgcc gcctttcctc
     9421 ccccgctata aaaagcagca ccggaaagga tgacccatgc ccggaatcgt ccgtctcagc
     9481 atttcgctcg aaaaaccttt acttgaccaa ctcgagcgac tggtgaaagc cagccggtac
     9541 acgaatcgct cggaatacct ccgcgacctg atccgtcagc ggctggtgga gcaggaatgg
     9601 tcggatcagc gccaggaagt aatcgggacc atcacgatgg tttacgatca tcggtctcgt
     9661 cttctctcgg ataagctggt ggagattcag cacgagcatc accggcatgt cctggccgcc
     9721 acgcacgtcc atctctcgcg ccgcctgtgt gcggaaatga tcatggttcg gggtagcgcc
     9781 tccagcctgc acgctttggt tgataagttg cggcagcagc gcggcgtgct gcacgccgaa
     9841 ttggggatga gcacgaccgg caagcacctc taatcccggt ctaatcgcgg agaatgccgg
     9901 ccagcaagcc cgcccgcctg ttggcgacaa agtatctccg aatttagcac gaattaagat
     9961 cggcgtcata ggtgccgtac atcgtggcct gtgacgagtc attatttgac gaggccgcga
    10021 tgagctgcgt tgacgagatt attggcgtgc accccgcgct gcccaggatc gattcagacg
    10081 ttttgcgtgg gtgcatccag gcctgcgccg cgtgtgggca ggcctgcgca gcctgtgctg
    10141 atgcgtgcct gtccgagccg cagtttgcca agctggtccg ctgcgtcagg ctgaacctgg
    10201 attgtgccga cctgtgtacc gcggcgacac atattctgct gcggcagacg cagcccgacg
    10261 cccgcgtgct gaccagcgtc ttgcaggccg tggtcgtcgc cgcgcgggtc tgtggaatgg
    10321 agtgcgatcg tcacgccggc cggcacgaac attgtcgcat gtgcgccatg gcctgccagc
    10381 attgcgaacg ggcgtgctgc gaactgctgc gggccggccc gtgaaaccag ggccggcgct
    10441 agccggcgtc agatcggccg gagagacgag gcgcgcggac gccgcggggc gttagggata
    10501 gttctcgggt ggcaggagtg agcgctttaa ttccagctta ctcatgaaat cccaccactc
    10561 atccacccat tcctggttga tcttcccgaa gcactcctcg atttcgcgct cgcgttgcga
    10621 ataactgatt tcctcgcctg cttcgcgggc cgcgattatg cggtaatact gggccaatcc
    10681 ggcgggcttt ttctcacgca tgtaataaac cagcgcccag cccattgggt agaagtaccc
    10741 gccgcctgaa taccacacct gactgtcaac catgaagagc ttgaggaagt cgggactcgg
    10801 ttttggataa tatctgcgga actggttcaa acgctcatgg ttgaacgctc caaagctgcc
    10861 gccttccgca ctgggtggga tttcaaattg catggtggtg ccctccacca gccaggtcgg
    10921 tttggcgggt aagcttatgt agtccatgaa agcaaatcgc ggaaacagcc cgatggcgta
    10981 gtggatgtga tgccccagtt cgtgctgcgt gacctccagc gtctggtatt cggcccagag
    11041 tttggccaga ttctcctcgc gcgtcctcac cgcgcccccg actctccggg cgtgctcgag
    11101 ccgcgcttta accgcgcgct cggacatcat gttgtagaag tgcgagcggt tcgtcacggg
    11161 cgtgaagaaa cccaaagcgc tcatgggccc accctcgacg ttcgtacata gagcgtcaaa
    11221 ttcctcgcgc gaatccacgt agtagagttc cagcttgtac tccggcaagc gcaccggcaa
    11281 gttgattatc cgcatgaacc tgatgttcca gcgccagatc gtttccagcc gccgcgcaat
    11341 gtctttcgca ttctgttcgg ggccggtgta taccaccacg acgtgtttgg tggtgacgat
    11401 gcccgtggcc ccgatgatgg ccttcatttc ttccaccgac tcttcgttca gcggcagctc
    11461 gccttcttcc agttccaaat cgctggccgg cagcacaatg ttgaagcctt ccagaagttt
    11521 ctcaatttca gcgtccggga tgccgctggc cccttcgggg tgctcgcttt tttctgccgc
    11581 ggctggtgat tcgccttcct cgaccggcag aacagccttg acctggttct tggggattgt
    11641 gactgtggcg ctgcgcgtga ctttgacgat gtactcggag ggcgtttcct caacctcacc
    11701 ggtgatccac gtcccatccg tgcgctggat acgtttcttc cccaaacttt ctgcctgggc
    11761 agtgccggag acgcagacaa gtagcagcat cgcgactaat aagcagattc cgcggcctcg
    11821 tggctgcgtc atggcaatac ccttcccgtg cttggcacgt aaatcgaact tgatacggca
    11881 tcatacaccg ccgccgtttg cgtgagcaag caatctttgg cagacccaaa gcaccacgcg
    11941 gcggggctcg ccgcgaccag tacgccaatg ccgcgctgtc tgctttagct tggcacctcg
    12001 gtgacttgcc cgtacactct gccacaaagt gcaaacgctg ttgagcgccg ccgcccggcg
    12061 acatcttatg cgggcccgat aatgacagaa caacaagact tcggcgagtt cgaactgctc
    12121 cgcaagctga tggaaacccc cggcgtttcc ggccgcgaag agcgcgtccg cgacctcata
    12181 ctggccgaaa ctgccggcct gtgggacgaa acccgggtag acaacatggg caacctcatc
    12241 tgcgttaagc gggcggtgac gagtgccaag cgtcgccgcg gcgcggccgc cgcgggaacg
    12301 gaggcccggg cggagccgct gcgcgtcatg ctggcctgtc atatggacga aatcggcttc
    12361 tacgtccgct tcattgatga gaacgggttt ttgcgtgtgc agaacgtggg cggcttcgac
    12421 gcccgaaatc tcttcgcccg ccgcgtgctg gtacagggca agcgcgatct ggtcggcgtt
    12481 ctgaacccgg cgggcaagcc ggtgcacctg gcgagcgagg acgagaagaa aaaaattccg
    12541 gagatgcgcg agttcttcgt cgatctcttt ctcactaaac ggcaggtaaa gaagctggtc
    12601 gacgtggggg atccggtcac gctgattcag cgcacggaaa tggtgggcga ttcgattacc
    12661 ggcaaggcgc tggacaaccg catcgcagtt tgggccgcga taagcgccct gcgccgggcg
    12721 gcgtcgaagc tgcgctatga cgtgtatttc gtcgcctgcg tgcaggagga ggtcgggctg
    12781 cgcggcgctg cgcccgcggc gtttggcatc gatccccacg ccggcattgc catcgacagc
    12841 accctctgct gtgacacgcc cggcacgagc aaggacgacg ccgtgaccga gttcggccag
    12901 ggcgtcggca tcaagatcat ggacggcgcc agcatcagcc atcgcggact tttcgacgag
    12961 ttcgtggccg tggcgcagcg aaaccggatc ccgtttcaac gcgaggtcct gccgcgcggc
    13021 ggcacggatg ccggcgccgt gcagcgggcc cgggccggca caaaggtcat cacactgtcc
    13081 atacccacgc gctacgttca cacggtcacc gaggccgtac acaaaaaaga cgcgcgggcc
    13141 gcggtggacc tgctcaccgc gtggctgagc actgagccgg caaagtcgat gactaatgca
    13201 aagcactaaa tgcaaaatga gcagagcagg ggggtcaaac gccgatggcc ggcaccggcc
    13261 gctcggccgt gttgcccagt gtcgcaggcg ctatgaataa atcggacccc gtccccgcct
    13321 tggtcgtcgc gtccgtattg atcgggctgg cgtgccccgc gggtgtgctg attgcgagtg
    13381 aaaccatgcc cacctgccaa acggccgccg atgccgttta cgttaacgcc aacatctaca
    13441 cgatgaacgc cgaccgcccg cgagccgagg cactggcagt tgcgggccag cgactcgtcg
    13501 cggtcggctc gaatgccgag atcctcaaaa ctgtcggccc cgcgacgcgc gtccacaatc
    13561 tgggcggaca aatggttctg ccgggcctga tcgattcgca ttgccacttc gccgcattgg
    13621 gcagctatag cctcgggcat gtggatctca gcggcgcgcg gtcgttcgac gaggtaatcg
    13681 aaatcgtcgc cgcgcgagcc gcgaaagccg aaaagggcac ctggattctc ggcggccgct
    13741 gggatcacga gaactggcgc gaccggcgcc tcccggcgca cgcggcgctg agtgcggcca
    13801 cgccggacaa tccggtctgg ttgcggcgcg tggatgtgca cgccggcatt gccaatgcgg
    13861 ccgcactgaa gctcgccggc attacgtccg caacgcccga gccgcccggc ggcgacattg
    13921 ttctggacga gcagggcgag cccaccgggc tgctcgtgga caaggccacc gaactgattg
    13981 agaacctcat tccggcggcg accgattcgg ccgcgatgat cctgaaagcg caggagctgt
    14041 gcttggcagt ggggctgacg ggcgtgcatg acgcaggcat ccagccgcct gaggtcgaca
    14101 tctaccgcaa gctcgcggaa cagggagccc tgaaaatccg ggtctacgcc atgatcgcag
    14161 gcgcggcggc gccccagtac ttccgcgagc acggcctgta tacaagcgag cggctaaccg
    14221 tccgcagcgc aaagttctac gccgacggcg ccttggggtc gcgcggggca tggctgctgg
    14281 cgccgtattc agaccggccg acagacgaag aaggccggcc ttatgtcggc ttgaatgtcg
    14341 tctcgccgga agagctgcga cgcgcggccg aggacgccct gcgccaggga taccaagtct
    14401 gtacgcacgc gattggcgac cgggcgaacc gggaagtgct gaacgcatac gaggtaataa
    14461 ggcaatcagg taacagcagc agccagggtg cttcccgcga ccatcgcttt cgcattgagc
    14521 atgcgcagat actggcgccg caggacctgc cgcgctttgc cgaactaggc gtcattgcgg
    14581 ctatgcagcc gacgcactgc acgagcgata tgcgctggat cgacgagcgg atcggttcgc
    14641 agcgctcggc cggggcgtat gcctgggcca gccttttacg ctccggcgta cgcttggcgg
    14701 gcggcagcga tttcccggtg gagtcgcata atcccttcct cggcctgtac gcagcctgca
    14761 cgcgccaaaa tgccgcgggc gagccggccg gcggctggca cgcgtccgag cgactgacgc
    14821 gtgaggaagc cttgcgggcc ttcacgctcg atgcggcgta cgcggcgttt gaagaaggtc
    14881 agaaaggatc gctggagccg ggcaaactgg cggacttcat tgtcatcgac cgcgacatca
    14941 tgacctgcga gccgcgcgaa atcgtggaaa cgcgggtgct gcaaacggtc attggagggg
    15001 aggcggtgta cagtgatgca gattttgcgg ccgaggggcc tgcgaaccac agataacaac
    15061 ctctcaccgc gcaggcggcg cgaacgccgc ttgcagcgcc agcatgctgt aggccgtggt
    15121 cagggccggg ctgccttcca tccagcggtc ggcttcgttg atccagcttc cgtccgggcg
    15181 ctgaagctcg gccagcttga gcaccaactc ttcgcgccag ttgtgctgcc ggccggagcg
    15241 gtcggtgatc accggctcgc cgaacgtggc caaagcgcgg gcgaaggtgt gatagtagta
    15301 gtagagtccc tcggccgatt gcgcctgcgg catgttgggg ttgtagtcca gcgtccagta
    15361 gttgcggatc caatcgagcg ccgcgcgcac gcgcggatca tcgcgcgtca agcctgcgta
    15421 aagcatgctc ttgaagcccg cgtaagtcat tgagccatag cagcgcagtt cctggcggcc
    15481 attgatttcc acggttccgg ctttggactc gccgttatgg gccgcggtgt agatgaaccc
    15541 gccctgggtc gagcctttgg cgaagggttg atcgttggtt tcgccgagca tctggcagcg
    15601 ctggacgaag atcagggctt ttttgtacgt cgggtcgtcg ggcggcaggc cactgtcacg
    15661 aagcgcgtcg agcatcatct gagtgttgga gaggtcgggg cgcttcgagc ggccataccc
    15721 ggacccgccg taccagggat catcgatgga tttgccttcc gcttcgtccc attggttttc
    15781 gatgaggaat ttctggagcg cagcgatctc cgagcggagc cggggcgtgt caccgcggga
    15841 ggggccagtt gtcgccgacg gcgcttcggg ggcggatttt ggagccgtcg cgggcgcttc
    15901 gggttcggat tcggccggaa gtgcggcaaa cattgaaagc gcgacgctgg attcgtagtt
    15961 cttgagcaaa ccctcggcgc cgtagacacc gccgtcctca cggataaatc tgcggacgaa
    16021 ttcgacgccg cggcgcactg aatcgtggtt cgggccaacc gtcggttcct ggatcagagc
    16081 cttcagagcc aggcacgtga tgcccggacc gccctgcgaa gtccaaccgc cgtcctgctc
    16141 ctgggccgcc agcaggaatt gtgcgccgcg gcggctaagc tcggccgcgc gctggcggag
    16201 ggcttcatca tccggttcac ccacgcggcc ggccggctgc gccaggacca tcgagctaaa
    16261 caacaacgtg aaggcaagaa cggcgaaacg gctcatagct aatctccgta gttatgccac
    16321 gggcggatta taagccgttg accgtttaat gaggcggctt cgcgtgaacg cgagctgcat
    16381 accgactccg aatgtcggcc ttgccccgcg caggggcatg cccgcgctta gagcttgggc
    16441 atggcaccca cgctcaactc gcgggggaac cattgcttat gcttaacgcc gcgtcgagca
    16501 acttgcgctg ctcgatcttg tgcagagtat gtgacccggt ggcggggctg ggcgaggccg
    16561 ggcggtagat gccgcggaag tcgaaccgct caaatgcccg cgaaagtggc gagcaacgaa
    16621 gataccgcca ggcgcccatg ttctccggtt cttcttgcac ccagagcagc gaagtcccgt
    16681 cgggatattg tgccagcgcc tgcgtcaccg cctcctcaga cagcggatag agttgctcga
    16741 agcgcacgat ggcgatgtcc cgctcggcct gccggttctt gcgctcctgc catagctcgt
    16801 agtaaatctt gccgctgcac agcagcaccc gccgcggcgg acggtcgcgc cgcacgtcgt
    16861 ccggcagaat gcggcggaag cggccgccgg ccagctcatc caggctcgag accgccaatg
    16921 catgccgcag caggctcttc ggcgtcatga cgatcaacgg tttgcgccac ggccgcaaca
    16981 cctgccgccg caacacgtgg aagtactggg cgggtgtcgt gggcacaacg acctgaatgt
    17041 tgtcctcagc gcacatggtc aggaaacgtt ccggccgggc gctcgaatgt tccggcccct
    17101 gcccctcgaa cccgtgtggc aaaagcagca cgaggccgtt catgctgtgc cacttctgct
    17161 cagcgctggc gatgaactgg tcaacgatca cctgggccgc attgacgaaa tcgccaaact
    17221 gcgcctccca tagcaccagt ccatcggggt aagccagact gtacccatgc tcgaagccca
    17281 ggacagccgc ttccgacagc gggctgttcc agatttcgac cggcgcctgg ccgcgttcca
    17341 ggtgcgccag cggtgcgtac gcggcgcccg tctcaagatc atgcaggacg gcgtgccgct
    17401 gcgtgaaggt accgcgctcg ctgtcttgcc cgctcaggcg aacccgcacg ccctcggtcg
    17461 ccagcgtcgc gaacgccagg gcttcggccg tggcccagtc cagcgggtgc tcgccggccg
    17521 ccatgttctt gcgcaaaatg agcaggcgtt cgatcttttt aagcgggtgg aaatccggcg
    17581 ggaccgtggc aatcctgtta agcagttcga ccagcctttg ccgcggaacg ccggtatcca
    17641 cctccggcac gctgctgtcg ggcccgccgc ggtaattact ccaaagctca ccgaggctgg
    17701 acgggcggcg cggctcaacg ccggtcgtgc cgctgcgcgc cccggccagt tcctcttcca
    17761 accgcttacg gcggtcatcg gcgatcttat ccgcttccgc ttgcgtgacc tcgccgagct
    17821 tcagcaggtg ctcgagatag ccgtcgcgga ccggtttacg ctcctggatc gtgcgataca
    17881 tcacgggctg ggtgaacgcc ggctcgtcgc cctcgttgtg cccgtgccgc cgatagcagt
    17941 acatgtcgat gacgacgtcg cgcttgaact gccgccggaa gtccatgctc agccgcacga
    18001 ccgtcgccac cgcctcgggg tcttcgccgt tgacgtggaa gatcgggctt tgcagcatct
    18061 tcgcgacatc ggaggcatag ggcgtggacc gcgcctgctc gggttcggtc gtgaagccga
    18121 gctgattgtt gacgatcacg tgcagcgttc cgccggtgcg gtaggcctct aaaccgctga
    18181 ggttcagcgt ttcctgcacg attccctcgc cggcgaaggc cgcatcgcca tggatttgaa
    18241 tggccatgca ccgctcgcgc gccgtgtcgg ccaggcggtc ctgcttcgcc cggcagcgtc
    18301 cggccacgac cggattgacg aactccaggt ggctggggtt aaaggccagc gcgaggtgga
    18361 cggtccggcc atcgctggac caatcgccgt gatagccgag gtggtacttc acgtcgccgg
    18421 ccggctgccg ccgcgactcg ttgtcttcaa attcacggaa gatgagtcgc gggctctttc
    18481 cgatgatgtt cgccagcacg ttcagccggc cccgatgggc catgccgatg acgatctcct
    18541 cgacgccctg gccggcggcg tgggcgatgg ccaggtcgag caggggaatg aggctctcgg
    18601 cgccttcgag cgaaaagcgc ttggccccga tgaacttttt atggatgaat tcctcgaaca
    18661 tcacggcgtc ggtaaggcgt gtgagtattc gaagctgctc gtcgcgcgaa agccgtaacc
    18721 ggttccgggt gccctccatc cgctgctgca accattcacg cacgtccgca tcgtcaatat
    18781 gcatgaactg cacgccgatc gagcggcagt aagtgtcgcg gagcaaagag attacgtcgc
    18841 gcaacgtacg caccggcggg ccggcaatcg tgcggctgga aaccggacga tcaaggtccg
    18901 cctcagtgaa ttcgtagaaa gacggttcta gccgctcatg tcccggccgc ggctggccga
    18961 gcgggtcgag ttgcgcgagc aagtgcccgc gcacgcgata ggcgcggacc aactggtcca
    19021 cacggtcctg gagctgctca gccgagggcg ccgctcgccc attcgccgtg cggccatcgg
    19081 gagccggcgg gttgaagata gtcgaaggtc gaaaggacgg cccgagtcgc ggtggagaaa
    19141 cggtgccatt cgccccgagc cgccggaaat actcggccca ctgcggcggg acgctgcccg
    19201 gatcgcgcgc gaagtcggca tacagccttt cgacgaaggc gacgttgacg gcgtccggca
    19261 agtcgtccgg cggggtacta gatctcgggt gcaacgatgc ggtcatggtt cacagcgcca
    19321 gtgacgaact agattaccac atgataattc taacagtcgc ccttcgctct gctcgccagc
    19381 cggtgcacct tcagagcccc agcccgcggc ccacagcccc gagttgtccc cgtatcgctg
    19441 ggtctcccgg tatcgacgcc ggccgtcggg agagcgcagc ggcgatcgcg agcgtccact
    19501 aaaatctcca gcccagagaa acctccggag agcgaatatg taccgactgg tgctgctgcg
    19561 gcatggcgag agcgaatgga acaaggaaaa ccgctttacc ggctggaccg acgtgggcct
    19621 gtcggaaaag ggcgtggcgg aagctcatga agccgcacag ctcttgatgc agggcgggta
    19681 caccttcgac gtcgcctaca catccgttct aaagcgcgcc atcaaaacgc tctggatcgt
    19741 cctggaagac atggacctga tgtggattcc ggtgcatcgc agttggcggc tcaacgaacg
    19801 tcactacggc gcgctccagg gcctcaacaa ggccgagacc gcggccaaac acggccccga
    19861 ccaggtcaaa atctggcgcc gcagctacga catcccgccg ccgccgctgt ccccggacga
    19921 cccgcgcaat ccggccaaag acccgcgcta cgcgcaattg cggcccgagg aaatcccact
    19981 caccgaagcc ctcaagaaca cggtcgagcg cgtcctaccc tattggcacg caaccctggc
    20041 cccggccgtt cgcaacggcc agcgcctgct catcgcgtcg cacggcaaca gcctgcgtgc
    20101 gctggtgaaa tacctcgaca acgtctccga cgccgacatc gttgagctga acatcccgac
    20161 cggaatcccg ctcgtctatg agctggacgc agaactcaag ccgctccggc acttctacct
    20221 cggcgacccg gccaaggtac aagccgcaat cgccggcgtg gcagcgcagg ccacgcaccg
    20281 gccgaagtga aagatcgcat cggcgtgaac gatcgcatta tcctaccgcc cggagggcgc
    20341 acgagcgttg ccagggacgt agtccctggt aggcgagccc cccaagcaga gccggggcgt
    20401 gccgccccgg agcgcgccaa caacctttca cacgcgcagc gtgatccgcg cccctccgac
    20461 ccgctccccg ctacagccac tttcccacct tcccaccttc ccacgttccc accggacagt
    20521 gaactgttcc cgcccggagg gcg
//
LOCUS       DAOSYS010000037        20188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2182_length_20188_cov_5.392639, whole genome shotgun sequence.
ACCESSION   DAOSYS010000037 DAOSYS010000000
VERSION     DAOSYS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2182_length_20188_cov_5.392639"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1334)
                     /locus_tag="PLU99_03800"
     CDS             complement(<1..>1334)
                     /locus_tag="PLU99_03800"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO16211.1"
                     /translation="QRRSGLEIRNIVQEPTYSTVTFRWNPRIVPTLEFNRPPYNPDGP
                     IVNNLFSLRWQAWDQHGGTRVQLYYDTDNQGYDGQPVPLPAGQTTLPKDGHSLSPVPD
                     LIKAGAYEIPAEIFYDTTYYFYARLIPGTGHDGQMEKACSEAWAYLSNRGRGHVADLT
                     VDTPPAPFEPQGKNKLEHWVVTCIDDSVPGAEVWSVTGSVSGLQVGTEGTTGNATTGV
                     PYTSDHHEVQFTIVSEAVAGSGRIEVEAGKAFLVDPTATFVASDFRARDTVRILEGSD
                     ATPGFYRVLSVPGPTRIELDGNPGPADAVSYRVHAFTAGLGDAIPDRFHFLTTGLTPY
                     SLPVTFHHKIDDDMEVVPSAYAWINVSYPDDATNPERRAPLRVLFDGSQSISERGETT
                     GLTYLWRFGDGGTSSEAITEHTFQSAFPDGVTVTLEVTSAGGAQGSASVVIV"
     assembly_gap    1335..1434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1435..4028)
                     /locus_tag="PLU99_03805"
     CDS             complement(<1435..4028)
                     /locus_tag="PLU99_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16212.1"
                     /translation="MDPQYDPASPDPAKRIPRPDPNQNPAPSGFMARFGNDKYDGPDY
                     WVNANNDGSKLQQQAVMPWMPSASTPAPDSFWAPFPWDYQAWWRAYCEDISQLMEDVG
                     EPDPPEWNNWIPIFREFDPANPNSPVFPGSERAFNPNCGGDTARADQTVDRCIPDEDV
                     TECDDADVGDPDPPNPGDGSVGGDASGGAGEIRPDAYGYYDGPAEFIDLASSMYHAAN
                     LDGTFNTSGLARGGDGRPGEVTSPHSNSPFGQDIGSGYPESPSGPDGSIPAGGPLAYN
                     VHGASGYDAGNVLNLEVLPWMWKKEVIQAIMGMEYHSARQYVYGSDVSLGRLATRHKL
                     ADGWESIPAQNPFGVPRIGPIALADGVLYGVRSEGSDLTARNYLVIIDTTRISDEFGT
                     VTGINGSATEIAEIESTEFDFPIGRITEMAYDAGAQLMYAIDDQFFQSFLWTIDLGTG
                     EATRVRSRQLIDEETGEWVEGPPLGALKGPYGTTFGASGLASAGTNLYTQDPASFYIC
                     RFERDERTQGFTGNLVTIDLGPVSVFAPFSVVALATQSAGLNPSLYALNESGEVYLIT
                     MSGMGWQISALPDVTFPSIGASPFLAGDRNLDGLLDMGEVRLAGTENYILDTLPLTPN
                     DGGPSSAYPFNRRRLTEDLVAALDEASDWDYVVMPAPQGDGSIKSFVHSTFYLPPGST
                     DGIVAAGGRPAFVLPAPAMDLPIFFRDESGDISPFPLWFSDFGIAIGSAGGETGLDQT
                     TLHIATMAHEWLHVWEGYPDLYDYDEYIGEHINYPVGIWDIMSGGFCHPSPPLKQFCL
                     GDARLGTQHAPWIQVNDLLRVMEPWTDTVVVLHDYAFHPVNSAWMFQNPDNTGERFYF
                     WRITQTEPT"
     gene            complement(4134..4976)
                     /locus_tag="PLU99_03810"
     CDS             complement(4134..4976)
                     /locus_tag="PLU99_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16213.1"
                     /translation="MAIALRTRTVLITGLLVAFAAVAAEVRAEERKFAVMLGVPIKTV
                     PPVGLPNPNDIWDAFFDQWKDGQPELPTPAIDSFAEYWYEISYGNVNVSGDVMGWATL
                     PWPVAPAEGATTDGVLNFRDLNINGEHDQFEGESFSQGRQLDLIDWNGDGVDGGGTGI
                     PDANWKAQVPTDDEYTRGFVDFGWRLVKSQDGYVTAIIDAVWTPGERYRDLNGNRRYD
                     ALVEPFRDGFGKPIPEGGCEQDGEIDAGEFCDVDGDGTWDFPEPFEDFLVIYDPNCGV
                     PEGC"
     gene            5565..6509
                     /locus_tag="PLU99_03815"
     CDS             5565..6509
                     /locus_tag="PLU99_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO16214.1"
                     /translation="MDDNGERRGEFGFRQAAPWLVALAVFLLTFFFLPALVGRISYAR
                     TAAQVRALREGMGGLERGDTLSTLFQAVAEAVKPAVVVIRTSERVRVEPEPDMRDFLR
                     RFFGEEGFNGFSGPKYYFKEGIGSGIIVDAENGYVLTNWHVVHKAQTAEVILADGRVR
                     QGRWAKSDQKNDLAIVKIRPGGLMAAPLGDSDEVKVGDWVLAIGAPEGLPQTVTSGII
                     SALGRSTAGGHESFLQTDAAINPGNSGGPLVNMAGHVIGINTAIISPVGVNAGIGLAI
                     PSNVARKTIRQLTDSASSTAQGHRDEDEAELITKQFSP"
     gene            6841..7890
                     /locus_tag="PLU99_03820"
     CDS             6841..7890
                     /locus_tag="PLU99_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO16215.1"
                     /translation="MFGLLTWMLAVAYYPREFPHWPTGWYWGMGAITAALLFASVLLH
                     ELGHAFVAQYYGIPVPSITLFIFGGVSQLAGEPPRALAEFLIAVAGPLVSLALAAVFG
                     AAAALWTDFPPVRALLLYLALINAMLVIFNLLPGFPLDGGRVLRALLWGMTRNYRRAT
                     TIAARVGQAFGILLIVFGIWQMFMGNFIGGMWMAFIGWFLQTAASAQVQQVALQRLLT
                     GHKVAEAMNTACPNVPPELTVRELVDEQVRRQGRRCFVVRRNGDTLGLLTLQGLKQAP
                     KAEWDRTRVDSVMSPLANTSYTVPDAELWSALEQMEREGVDQLAVVASGQLVGMLSRD
                     DVVNHMNVLRAVEGP"
     gene            complement(7954..8514)
                     /locus_tag="PLU99_03825"
     CDS             complement(7954..8514)
                     /locus_tag="PLU99_03825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012901645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOO16216.1"
                     /translation="MNKVHILGFSGSLRQGSYNRALLRAAQELLPEDVRLELFDLGPI
                     PLYNGDVEARGFPEAVVAFKERIAAADALLIAAPEYNYSMPGVLKNAIDWASRPPDAS
                     PLNGKPVALMGASVGLLGTARSQYHLRQVCVFTNMLPLNRPEVFVGRAHEKFDAQGRL
                     IDENTRKHVKGLLEAVRDWTRRLRCE"
     gene            complement(8511..11321)
                     /locus_tag="PLU99_03830"
     CDS             complement(8511..11321)
                     /locus_tag="PLU99_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HOO16217.1"
                     /translation="MARKTAGDIEVRRLAEDARRDKDWKRWGPYLAERQWGTTREDYS
                     AAGNPWRYFTHEHARSRAYRWGEDGLLGFTDRQCRLCFALALWNGRDPILKERLFGLT
                     NPEGNHGEDVKECYFYLDATPTYTYAKALYKYPQAEFPYEQLVAENGRRNRYEREFEL
                     QDTGIFDERRYFDVLAEYAKNSPNDVRIRISLSNRGPERAALHVLPTLWFRNTWSWGC
                     GFEGCWPKPRIALTGPSELTCDHETLGRFHLRVGPAPNGEGPRFLFTDNETNTQRLYR
                     VPNENPYVKDAFHQYVIHNQTGAVNPQQTGTKAAAYYVLEIPPDSTVVLRLRLFDEEE
                     RPAQPFGPQFERIFTQRVKEADAYYAEVIPKKASEDEQRISRQAYAGLLWSKQFYHYV
                     VHNWLEGDPLQPPPPPRVHGRNRNWTHMFNRDVVSVPDKWEFPYYASWDLAFHMLPLA
                     QLDPHFAKQQLILFLREWYMHPSGALPAYEFNLDDANPPVHAWACWRVYKITGQRGQR
                     DRSFLERVFHKLLINFTWWVNRKDRTGKHVFSGGYLGLDNIGPIDRSMPLPGGGHLQQ
                     ADATAWMAFYCLTMLAIAMELAVEDPAYEDVASKFFEHFVAIADAINTLNGTGLWDEQ
                     DGFYYDHARVNGRSSRLPFRSMVGLIPLLAVEVLDDEAVNRLPSFRKRMQWFLDNRRD
                     LAGRISCFTSRKVEGRSGRLLALPARPRLERVLRYMLDENEFLSPYGIRSLSRVHAGE
                     PCMCLSGGDEFPVEYQPGEDTTGLFGGNSNWRGPVWFPVNYLLIEALERYHRYYGDDL
                     LVEFPTGSGRTMNLKQVARELSERLVRIFRADEQGRRVLHGSDYRFADDPHWRDLLLF
                     HEYFHGENGRGLGASHQTGWTALVTRLLEDQYKAPPAAPKRVRKPRAAKTAEPVATPS
                     AGRPAKRGKAGASRTSRSKRKA"
     gene            complement(11355..12470)
                     /locus_tag="PLU99_03835"
     CDS             complement(11355..12470)
                     /locus_tag="PLU99_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOO16218.1"
                     /translation="MKAMAVFPATREIKLIEHPEPQIAAPTEVKLRMLEVGICGTDRD
                     IATFVYGTPPPGEDYLIIGHESLGEVLEVGAQVQNFRPGDLVVLMVRRPCPDPHCMAC
                     RAGRPDFCFTGRYKERGIKELHGYMTEFVVDDQKYMNLVPRELRQVAVLTEPLTIAEK
                     GLAQVLLVQQRLPWGCPQPAQGEPGYCHQALVLGAGPVGLLGALTLRRAGFKTCVYSR
                     VFDPQRIGQLLESIGAEFIAAPETPLKALPGRIGNIDLIFEATGASTLAFDAMPFLGT
                     NAVFVFTGVPGRKGPISVEGNLIMRDLVLKNQIVFGTVNADRSAFEAAIRDLGVFTSR
                     WPNTVPALITGRHPIEAAPEFLVKLTPGIKQIITISG"
     gene            complement(12507..12986)
                     /gene="rpiB"
                     /locus_tag="PLU99_03840"
     CDS             complement(12507..12986)
                     /gene="rpiB"
                     /locus_tag="PLU99_03840"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOO16219.1"
                     /translation="MRIAIAADHAGFDLKEFIRRELTTAGYETIDLGTRSADQPDDYP
                     DFAAALGRAIQTGQAERGVLICGSGVGASVAANKLVGIRAGLCHDTYSAHQGVEHDDM
                     NVLTLGARVIGPALAAELVFTFLRAAFSGAERHARRLAKVRALEEHCLGRTADVCGA"
     gene            complement(13051..>14307)
                     /gene="zwf"
                     /locus_tag="PLU99_03845"
     CDS             complement(13051..>14307)
                     /gene="zwf"
                     /locus_tag="PLU99_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOO16220.1"
                     /translation="EADLAGTDGLRSLAGRVQALERATRLPGNRVFYLAVAPHFFAPT
                     IAALDEAGLVRRNVERPWARVVIEKPFGHDLASARELDRYIETFLRPDQVYRIDHYLG
                     KETVQNLLAVRFGNSVFEPLFRRELVEFIQITVSETAGMEGRRGAYYDRAGALRDMLQ
                     NHLLQLLALVTMDPPATLTAGDISDAKLKVLRTLVPPTGAEVERQVVRGQYGPGVVDG
                     RAVRGYREEEGVSPNSSTETFVALRAEIASWRWAGVPLLLRTGKRLARRATEIEVQFK
                     LPPLQLFQTVECEGDLCIESPGQPATLQFRIQPNEGISLSFSAKRPGMLMDLEPVCFD
                     FNYQRLGRELPEAYERLLLDALRGDATLFPRSDELEASWKFVAPILEAWSAGPPPKFP
                     NYAAGSWGPPEAQRLAEGRLGGWKCR"
     assembly_gap    14308..14407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14506..17400)
                     /locus_tag="PLU99_03850"
     CDS             complement(14506..17400)
                     /locus_tag="PLU99_03850"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOO16221.1"
                     /translation="MQVITESLPARTPVANPLLELRRYGQSIWLDYIRRHLITSGELA
                     RLGEEDGLCGVTSNPSIFEKAVTGSTDYDDILSTAARRGERDPLLVYELLAVRDIQGA
                     AEVFRPLYEQLRRRDGYVSLEVSPLVAHDTQSTLEQARRLWREVGWDNVFIKVPGTKE
                     GVPAIRQLISEGINVNITLLFAREMYEQVAEAYLAGLEQFAARGGDVARVASVASFFV
                     SRIDTAVDALLTERLRAAGGEDHSKLRWLLGKVAIANAKLAYQTYKEIVRSQRWQKLA
                     RKGAMPQRLLWASTSTKNPSYRDVLYVEELIGPDTINTMPPATMDAFRDHGRVRPSLE
                     EDVEEAREVLETLAQVGISLPEVTQKVLDDGVQLFSESFERLISAIREKIKGTGRPAM
                     KRMSYHLPQPLAEDVESTLQSWHAEGKVRRLWARDAGLWTGRDEGHWMDWLAVTEDQP
                     AEGARFEEVAEEIQSAGFTRAVLLGMGGSSLAPEVLAQTFGPQAGHPVLLVLDSTDPD
                     EIRAVERQIDFARTLFIVSSKSGTTLEPNILKDYFFERVRAAVGEAEAGRRFIAITDP
                     GSKLERVAEGNAFRRLFHGVPGIGGRYSALSNFGLIPAAIMGLDVPRLLDTAEEMAQA
                     CRSCVPAEENPGVVLGVIMGVLARNGRDKVTVIASPGVAGLGAWLEQLLAESTGKDGD
                     GVIPVDREPLGPPEVYDTDRLFVYLRLSGTPDNGQDAAVDALQRAGHPVVRITMQDAY
                     CLGAEFFRWQIATAVASAVLGVNPFNQPDVEEAKLQARKLTAAFEEDGALPREEPILR
                     GDGLSFFADEKNASALMYAAGTDRSPRGLLKAHLKRLGSGDYFALLAYVERNAFNSEQ
                     LGTMRRLVRNARRVATCGGFGPRYLHSTGQAYKGGPNTGVFLLITHEQASDLPVPGHK
                     YTFGAVQAAQARGDFQVLAERDRRILRVHLGPDVPAGLEALTELTREAVQ"
     gene            complement(17435..18193)
                     /gene="pgl"
                     /locus_tag="PLU99_03855"
     CDS             complement(17435..18193)
                     /gene="pgl"
                     /locus_tag="PLU99_03855"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOO16222.1"
                     /translation="MYPSSLIVLDNGTALAAQAAEFIARAAREAVQERGRFMLALAGG
                     KTPEETYRHLAEPQQVSAMPWENTHLFFGDERFVPPDDERSNFATVRRTLLAGVPIPA
                     SQVHSVPTTLGSAAEAAAAYSEELAQHFTGCEHAPPRFDLILLGLGEDGHTAGLFPHA
                     PVLRITNEWVTWSPPGKLPPPVDRITFTYPVLNASRSVLFLVCGAKKAAVLRDVLEER
                     RRPAQRPAAGVKPQRGSLTWMVDEEAAQMLTCRA"
     gene            complement(18317..18847)
                     /gene="folK"
                     /locus_tag="PLU99_03860"
     CDS             complement(18317..18847)
                     /gene="folK"
                     /locus_tag="PLU99_03860"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOO16223.1"
                     /translation="MMARAFIGVGSNVDPERNVLLGIQRLAQHVRVAGVSTVYRTAPI
                     GRPEQADYYNCVVAVETALPPLELSQLLHEIERELGRRRTDDRYAARCIDLDLIVYDD
                     LVLHSAELKVPDPDILTRAFLAAGLCELAPTLVLPDSGRSVRAIAAELQTSGLTPLPS
                     YTTRLREVVRQIEPRT"
     gene            complement(18844..19200)
                     /gene="folB"
                     /locus_tag="PLU99_03865"
     CDS             complement(18844..19200)
                     /gene="folB"
                     /locus_tag="PLU99_03865"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOO16224.1"
                     /translation="MDRIRIRDLSVRCIIGVEDAERRQKQDVIINLVIYTDLQRPGRS
                     DDFADALDYSALKKRVVALVENSHRRLLEALAEDIARLCLEYPAVHAVRVSVDKPGAL
                     RFARSVAVDIKRRRGE"
     gene            19335..19997
                     /gene="folE"
                     /locus_tag="PLU99_03870"
     CDS             19335..19997
                     /gene="folE"
                     /locus_tag="PLU99_03870"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOO16225.1"
                     /translation="MSSAASKLSTEELPPGAAATSRRLSEQQARTFEGYIAEILAACG
                     LNLELPPTADTPRRFLQALLDATEGYEGDPKLVRVFDTECRGGSDCRLSQIIEGPIPF
                     ASLCEHHAFPFYGRVYIGYIPHEHIFGLSKLVRLVRLYTRRFAVQERIGRQIADTMVE
                     ILQPHAVAVYVTARHMCTQMRGVRAVAPHTRTTVWRGEYERNRSLRAEFLAACGVGQR
                     TA"
     gene            20047..>20188
                     /locus_tag="PLU99_03875"
     CDS             20047..>20188
                     /locus_tag="PLU99_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOO16226.1"
                     /translation="MIRTQRVYEKQGNGGAHYLVERLWPRGVRKEALRLDGWLREAAP
                     STE"
BASE COUNT         3711 a         6515 c         6011 g         3751 t
ORIGIN      
        1 ccacgatcac gacgctcgcc gaaccctgcg ccccgccggc actggtgacc tccagtgtga
       61 cggtcacccc gtcggggaag gcgctctgga aagtgtgctc ggttatagcc tcgctggacg
      121 taccgccgtc gccgaagcgc cagaggtatg tcaggccggt cgtctcgccg cgttcggaga
      181 tagactgcga accatcgaac aatacccgca acggcgcgcg gcgttcggga ttcgtcgcgt
      241 catcggggta cgagacgttg atccatgcgt acgcactcgg gaccacctcc atgtcgtcgt
      301 cgatcttgtg gtggaacgtg accggcaggc tgtagggcgt aaggcccgtg gtcaggaagt
      361 ggaagcgatc cggtattgcg tctcccaggc cggccgtgaa ggcatgtacc cggtaagaaa
      421 cggcgtccgc cggaccaggg ttgccgtcca gctcaattct ggtcggcccc ggcacggaaa
      481 gcacgcggta gaaaccggga gtcgcatcgc tgccctccag aatgcgcacg gtgtcgcgag
      541 cgcggaagtc gctcgccaca aatgtcgccg tgggatcaac gagaaacgcc ttgccggcct
      601 cgacttcgat ccggccggag cctgcaaccg cctcggacac tattgtgaac tgcacttcgt
      661 ggtgatccga cgtatacgga acgccggtcg tggcgttgcc ggtggttcct tccgtgccca
      721 cctgcaggcc ggacacggag ccggtcacgc tccatacctc ggcgcccgga accgaatcgt
      781 caatacacgt cacgacccag tgctcaagtt tgttcttacc ctgcggctcg aagggcgccg
      841 gcggagtatc gaccgtcaga tccgccacat gcccgcgccc gcggttggag aggtaagccc
      901 aggcttccga gcacgctttc tccatctgcc cgtcgtggcc tgtaccggga atcaggcgcg
      961 cgtagaagta gtaggtggtg tcataaaaga tttccgcggg aatttcgtaa gcgcccgctt
     1021 tgatcaggtc cggaaccggg gaaagtgaat gcccgtcctt gggcaacgta gtttgccccg
     1081 ccggcagcgg cacgggttga ccatcgtacc cttgattgtc ggtatcgtag tacagttgca
     1141 cgcgcgtgcc gccgtgctga tcccaggcct gccagcgcaa actgaacaaa ttgttgacga
     1201 tgggcccatc cgggttgtac ggcggacgat tgaattccag cgtcggcaca atccgcgggt
     1261 tccatcggaa ggtgacggtg gagtaagtgg gttcctggac aatgttgcgg atttccagcc
     1321 cgctacggcg ctgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgggt
     1441 tcggtctgcg tgatgcgcca gaagtagaag cgttcgccgg tattgtccgg gttctggaac
     1501 atccacgccg agttaaccgg atggaatgcg tagtcgtgca acacaaccac cgtgtcagtc
     1561 caaggctcca tcacgcggag cagatcgttg acttggatcc agggagcgtg ctgcgtgccc
     1621 aggcgggcgt ctccgaggca gaactgcttc agcggcggcg acgggtgaca gaacccgccg
     1681 gacatgatgt cccagatacc caccgggtag ttgatatgct cgccgatgta ttcatcatag
     1741 tcgtacaggt caggatagcc ctcccacacg tgtagccatt cgtgcgccat cgtggctatg
     1801 tgcagggtcg tctggtccag tccggtttcg ccgccggcgc tgccgatcgc tatgccgaaa
     1861 tcagagaacc acagggggaa cggggaaatg tccccagatt cgtcgcggaa gaagatgggc
     1921 aagtccatag ccggcgcagg cagcacgaat gccggacgcc caccagcggc tacaatgccg
     1981 tcggtggagc ctggcggcag gtagaaagtc gagtgcacga agctcttgat gctgccatcg
     2041 ccctgtgggg cgggcatgac cacgtagtcc cagtcggaag cttcatccag ggcggcgacc
     2101 aaatcctccg tcagccgccg acggttaaag ggatacgcgc tcgatgggcc gccgtcgtta
     2161 ggcgtcagcg gtaatgtgtc gagtatgtag ttttcggtgc cggccaggcg cacctcaccc
     2221 atgtccaaca ggccgtccag gttgcggtcg ccggccagga atgggctggc cccgatggac
     2281 ggaaacgtaa cgtcgggcag ggccgatatt tgccacccca tcccagacat cgtaatgaga
     2341 tacacctcgc ccgactcgtt gagagcatac aggcttgggt tcaagccggc gctttgggta
     2401 gcaagcgcca ccacgctaaa cggcgcaaat acggagaccg gccccaaatc gatggtaacc
     2461 aggttccctg tgaaaccctg cgtacgctcg tctctttcaa atctgcaaat gtaaaaggac
     2521 gcgggatcct gcgtgtagag attcgtgccg gcggacgcca agccacttgc gccaaacgtt
     2581 gtaccgtatg ggcctttgag ggcgccgagc ggcgggccct cgacccactc gcccgtctcc
     2641 tcgtcgatga gctggcgtga ccttacccgc gtcgcctcac cagttcctaa atcgatggtc
     2701 cagagaaaac tctggaagaa ctggtcatcg atcgcgtaca tcagttgcgc gccggcgtcg
     2761 tacgccattt ccgtgatgcg cccgatggga aaatcaaact cagtgctttc tatttccgca
     2821 atctcggtcg ctgagccatt gatgccagtg accgtgccga actcatccga gatacgcgtg
     2881 gtatcgataa tcaccagata attgcgcgcc gttaagtcgc tgccctcgct tcgcactcca
     2941 tagagaaccc cgtcggccaa cgcgattggc cctatacgcg gcacgccgaa cggattctgt
     3001 gcgggaatcg actcccaccc gtcggcaagc ttgtgtcgtg tggccagacg cccaaggctg
     3061 acgtcggagc cgtagacata ctgccgggcg gaatggtatt ccatgcccat aatcgcctgg
     3121 atgacctctt tcttccacat ccagggcaaa acttccagat tcagaacgtt gcccgcgtca
     3181 taaccgcttg caccatggac gttgtacgcc agcggcccgc ccgcggggat cgaaccgtcc
     3241 ggccccgaag ggctttccgg atatcccgag ccaatgtcct ggccaaacgg gctgttgctg
     3301 tggggcgagg taacttcgcc cggacgaccg tcgccgcccc gcgccagtcc ggacgtgttg
     3361 aacgttccgt ccaggttggc ggcatggtac atcgacgaag ccaggtcgat gaattccgca
     3421 gggccatcat aatatccgta agcgtccggc ctgatttctc cggcgccgcc ggaggcgtcg
     3481 cccccgacgc taccatcgcc gggattcggc gggtcgggat cccccacgtc ggcatcgtcg
     3541 cactccgtca cgtcttcgtc gggaatgcat ctgtcaacgg tctgatcagc gcgagccgtg
     3601 tcaccgccgc agttcggatt aaaggcgcgt tcggaacccg ggaacaccgg cgaatttgga
     3661 tttgccggat cgaattcgcg aaagatcggg atccagttgt tccattcggg tgggtcaggc
     3721 tcgccgacgt cctccatcaa ctggctgatg tcttcacaat atgcccgcca ccaggcctgg
     3781 tagtcccaag ggaaaggcgc ccaaaagcta tccggggctg gagtagaggc cgacggcatc
     3841 cacggcatca cggcttgctg ctggagcttc gacccgtcgt tgttcgcgtt aacccaataa
     3901 tccggcccat cgtacttgtc gtttccgaag cgggccatga agccggaggg tgctgggttt
     3961 tggttcgggt cggggcgcgg aatgcgcttc gcagggtcgg gactggctgg atcgtactgc
     4021 gggtccatcc ggatcggctc tcccgcgtcc ccaggatagt tggcgcggat aaaggcctcg
     4081 gcccaagcgc ggttacttgc atcgaggttc ttccaactcg ggtcaagctt aactcagcag
     4141 ccttctggta cgccacagtt gggatcgtaa atgaccagga agtcctcaaa cggctcgggg
     4201 aaatcccaag tcccgtcgcc atcgacgtcg caaaactctc ccgcgtcgat ttcgccatct
     4261 tgctcacacc ccccctcggg gatgggtttg ccaaaaccat cccggaacgg ctcgacgagc
     4321 gcatcatagc ggcggttacc gttgaggtcg cgataacgct cacccggcgt ccaaaccgcg
     4381 tcgatgatgg ctgtgacgta gccatcctga gatttgacca ggcgccaacc aaagtctacg
     4441 aacccacgag tgtactcgtc atcagtggga acctgggctt tccaattcgc atccggtata
     4501 cccgtgccgc ctccgtctac accgtcgcca ttccaatcta taaggtcaag ctggcggccc
     4561 tggctgaacg actccccctc gaactggtcg tgctcaccat tgatattgag atcgcggaag
     4621 ttcaggacgc catcagtcgt cgcaccctcg gccggagcta cgggccaggg cagtgtggcc
     4681 cagcccataa catcgccgct gacgtttacg ttgccataag aaatctcgta ccagtactcg
     4741 gcgaacgagt ctatggccgg agtaggcagt tcaggttgcc cgtccttcca ttggtcgaaa
     4801 aaagcatccc aaatgtcgtt agggtttggc aggccaacgg gaggaacagt ttttatcggc
     4861 acgcccaaca tgaccgcaaa cttccgctcc tcggcccgaa cttcggcagc cacggcggca
     4921 aaggcgacaa ggagaccggt aatcaaaacg gttcgggtgc gcagagcgat ggccatcatt
     4981 gcttcctagt gagaaccaca gttcggaccg ccagacccac aggcctggac ggcgaatttt
     5041 tgcgtgcccc cgtttggcac tatctatccc cttctgtcaa aacaaggcga gcgttggccg
     5101 tgtcaggcgg cgaacgcgcg caatgctaca gttccttgga ctaccgcatc ctaccttcgc
     5161 cctgacagtc cgtcaatctg aaacgctccc acgatgacgg attcccgtcg ccccctctgg
     5221 gcgccgccgc aggcctacat attcgtcgcc ccacgcatac atattcatta ttaaaacgcg
     5281 ccaaccagca accggcgcat acggccaaca gttataggaa cgtggactgc ccatctcgcg
     5341 cgctttgaat ccccgagcca gcgtcttgaa ttcccgagcc agcgtctccc gaattgaaat
     5401 cctgagccag catctcccga atgccaatac taataactaa tgactaacaa ctaatcacga
     5461 ccgcccaagc actgccaaca acccactttg ctcccccctt cgttgcgggc caggcgcggg
     5521 gctcggaata gaatccccgc gcaaccggcg acacgagaac tgccatggac gacaacggcg
     5581 aacgccgcgg cgaatttggt tttcggcagg cggcgccgtg gctggttgcg ctggctgtct
     5641 tcttgctgac gttcttcttt cttccggcct tggtcggtcg catttcgtac gcacgaaccg
     5701 cggcccaggt gcgggcgctg cgggagggca tgggcgggct ggagcgcggc gatacgctct
     5761 ccacgctctt ccaggcggta gccgaagcgg tcaagccggc cgtggtcgtg atccgcacca
     5821 gcgagcgtgt gcgcgtggag cccgaaccgg acatgcgcga tttcctgcgt cgctttttcg
     5881 gcgaggaggg cttcaacggc ttctccgggc caaaatatta ctttaaggaa ggcatcggca
     5941 gcgggataat cgtcgatgcc gagaacggtt acgtacttac caactggcat gtggttcaca
     6001 aggcccagac ggccgaggta attctcgcgg acgggcgcgt gcgccaaggc cggtgggcca
     6061 agagcgacca gaagaacgat ctggcgatcg tgaaaatccg cccgggcggg ctgatggcgg
     6121 cgccgttggg cgacagcgac gaggttaagg tgggcgactg ggtgctggcc atcggcgccc
     6181 ccgagggatt accgcagacc gtcacctccg gcatcatttc agccttgggg cggagcaccg
     6241 caggcgggca cgaaagcttt cttcagacgg acgccgccat caaccccggc aactccggcg
     6301 gcccgctggt taacatggcg gggcacgtga tcggcattaa tactgcgatc atctccccgg
     6361 tcggcgtgaa cgccggcatc ggactggcga ttccgtcgaa cgtcgcgcgg aagacgatac
     6421 gccaacttac cgactcggcg tcgtccacag cgcaagggca cagggacgag gacgaggcgg
     6481 agttgattac gaagcaattc tcgccctgag ccgcgggaag gcgaagctcc cgctgagccg
     6541 ggggtgctca aagcgctgac ggcgaattaa cggccctcaa cagaccactg ccggcctcac
     6601 ccatatactc gatgagcttt gatttgaaag ggctaatctc tgaataacga agctgtgcca
     6661 gctccgttct ccccagatgg tgccggatac gaatcgggcc ggcccgcgcg cgatgggcca
     6721 cggtcgtcgc acgagtccgc caacccgaaa cgcagcggca gaggctttct gtccgcacgt
     6781 tcaatcccgc ttggtcgcat ccgaggtatc cgcatcgcgc tggatgcctc atggttcatc
     6841 gtgttcgggc tgctgacgtg gatgctcgcc gtcgcgtatt acccgcgaga atttccgcac
     6901 tggccgactg gctggtattg gggcatgggc gcgatcacgg cggcgttgct gttcgctagt
     6961 gtgctcctgc atgagcttgg gcacgcgttt gtggcgcagt actacggcat tccggtgccc
     7021 agtatcacgc ttttcatctt tggcggggtc tcgcaactgg ccggggagcc accgcgcgcc
     7081 ctggcggagt tcctgatcgc cgtggccggg ccgctggtaa gcctggcatt ggcggcagtc
     7141 ttcggggcgg cggcggcgct gtggacggat tttccgcccg tacgagccct gctgctgtac
     7201 ctggcgctga tcaacgccat gttggtgatc ttcaacttgc tcccggggtt cccgctggac
     7261 ggcggacgcg tgcttcgcgc cctcctctgg ggcatgacac gcaattaccg ccgcgcaacc
     7321 acgattgcgg cccgcgtcgg ccaggccttc ggcatactgc tcatcgtgtt cggcatctgg
     7381 cagatgttca tggggaactt catcggcggc atgtggatgg cgttcatcgg ctggttcctg
     7441 caaacggccg caagcgcgca ggtgcagcaa gtcgcgctgc agcgtctgct gacggggcac
     7501 aaggtggccg aggcgatgaa cacggcctgt cctaacgttc cgccggagct gacggtgcgc
     7561 gagttggtcg atgagcaggt tcgtcggcag gggcggcgct gcttcgtggt acggcggaac
     7621 ggcgacaccc tgggcctgtt gacgctgcaa ggtctgaagc aggcgccaaa ggccgagtgg
     7681 gaccggacca gggtcgacag cgtcatgtca ccgctggcca ataccagcta caccgtaccg
     7741 gatgccgagc tctggtcggc tttggagcaa atggagcgcg aaggcgtcga tcaactggcg
     7801 gtggtcgcct ccggccaact ggtcggaatg ctcagccgtg acgacgttgt aaatcacatg
     7861 aacgtgttgc gggccgtcga gggtccgtag gagtggaggc gggtccgcgg cctctcgcgg
     7921 cgcgggccag gtaagtcgca gcgcgcgccg gggtcactcg cagcgcagcc tgcgcgtcca
     7981 atccctcact gcctcgagca ggcccttcac gtgtttgcgc gtgttttcat caattaaccg
     8041 gccctgtgcg tcgaatttct cgtgtgcccg gcccacaaat acctccggcc gattcagcgg
     8101 cagcatgttt gtgaacacgc acacctggcg caagtggtat tgcgagcgcg ccgtgcccag
     8161 caggccaacg gacgcgccca tcagtgcgac gggcttacca ttgagaggtg acgcatccgg
     8221 cgggcgcgag gcccaatcga tcgcattctt cagcacgccg ggcattgagt aattgtactc
     8281 gggagcggcg atcagcaggg cgtcggccgc ggcgatgcgc tccttaaatg ccaccaccgc
     8341 ttcgggaaag ccgcgggctt caacatcccc gttgtacagc ggaatcgggc cgaggtcgaa
     8401 gagctccagc ctcacgtctt cgggcagcaa ctcctgtgcg gcacgtagca gcgcccggtt
     8461 atacgagccc tggcgcagac tgcccgagaa tccgagaata tgcactttgt tcatgccttg
     8521 cgtttgctcc tggaagttcg cgatgcgccc gctttgcccc gcttcgctgg gcggcccgcg
     8581 cttggcgttg cgacaggctc ggcagtcttc gcggcccgcg gtttgcgcac gcgtttgggc
     8641 gcggccggcg gagctttgta ctgatcttcc agcagccggg tcaccagggc tgtccagccg
     8701 gtttggtgac tggcgcccag cccgcggccg ttctcgccgt gaaagtactc gtgaaacaaa
     8761 agcaggtcgc gccagtgtgg gtcatcggcg aagcggtagt cggacccgtg cagcacgcgc
     8821 cggccttgct cgtcggcccg gaaaatccgc accagccgct cgctcagctc gcgcgccacc
     8881 tgtttcaggt tcatcgtccg ccccgacccc gtggggaact ccaccagcag gtcgtcgccg
     8941 tagtaacggt gataacgctc aagcgcttcg atcagcaggt aattcaccgg aaaccagacc
     9001 ggcccgcgcc agttcgagtt gccgccgaac aatccggtcg tgtcttcgcc cggctgatat
     9061 tccacgggaa actcgtcccc accgctcagg cacatgcagg gctcgccggc gtgtacgcgc
     9121 gaaagcgaac ggatgccgta aggtgaaaga aattcgttct catccagcat gtagcgcagc
     9181 acacgctcca gccgcggccg cgcgggtagg gccaaaaggc ggccactgcg gccctcgacc
     9241 ttgcgcgaag tgaaacacga gattcgcccg gccaggtcgc ggcggttatc cagaaaccac
     9301 tgcatccgct tgcgaaagct gggcagccgg ttgaccgcct cgtcgtccag cacttcgacc
     9361 gccagcagcg gaatcagccc gaccatggac cgaaagggca ggcggctgga tcggccattg
     9421 acccgggcgt ggtcgtagta gaatccgtcc tgctcgtccc acaggcctgt cccgttcagc
     9481 gtattgatgg catcggcgat ggccacgaag tgctcgaaga acttcgaggc gacgtcctcg
     9541 taggccggat cctcgaccgc cagttccatg gcaatggcca gcatggtcag gcagtagaac
     9601 gccatccagg cggtggcgtc ggcctgttgc aggtgacctc cgccgggcag cggcatggaa
     9661 cggtcgatgg gaccgatgtt atccaagccg agatagccgc cgctgaacac gtgtttaccg
     9721 gtacggtcct tgcgattgac ccaccaggtg aagttgatca acagcttgtg aaagacgcgc
     9781 tccaggaagg accgatcgcg ctgtccgcgc tgcccggtga tcttgtagac tcgccaacag
     9841 gcccaggcat gcacgggcgg gttggcatcg tcgagattga attcatatgc cggcagggcg
     9901 ccggaggggt gcatgtacca ttcgcgcagg aataggatca gttgctgctt ggcgaaatgt
     9961 ggatccagtt gcgccagcgg cagcatgtgg aatgccaggt cccaggacgc gtagtagggg
    10021 aactcccatt tgtcgggcac cgacaccacg tcgcgattga acatgtgcgt ccaattgcga
    10081 ttgcggccgt gcacgcgcgg aggcgggggc ggctggagcg gatcgccttc cagccagttg
    10141 tgaacgacat agtggtagaa ctgcttggac cacaatagcc cggcataggc ctggcggctg
    10201 atgcgctgtt cgtcttcact ggccttcttg ggaatcactt ccgcgtaata agcgtcggct
    10261 tccttgacgc gctgggtgaa gatgcgctcg aattgcgggc cgaaaggctg cgccggccgc
    10321 tcctcttcgt cgaacagccg caatcgcagc actaccgtgc tatccggcgg aatctccagc
    10381 acgtaatatg cggccgcctt ggtaccggtc tgttgcgggt ttaccgcccc agtctggttg
    10441 tggatcacgt actgatggaa cgcgtccttc acgtaggggt tctcgttggg cacccggtac
    10501 agtcgctgtg tattcgtctc attatcggtg aagaggaagc ggggcccttc gccatttggc
    10561 gccgggccga cgcgcaagtg aaagcgcccc agcgtctcgt gatcgcacgt cagctcgctc
    10621 gggccggtca gtgcgatccg cggcttgggc cagcacccct cgaacccgca gccccacgac
    10681 caggtgttgc gaaaccataa agtcggcaac acgtgcagcg ccgctcgttc cggtccgcga
    10741 ttgctcaggc tgatccgaat gcgtacatca tttggcgagt tcttggcgta ctcggccaga
    10801 acgtcaaagt agcgccgctc gtcgaagatg cccgtatcct gcagctcaaa ctcgcgttca
    10861 tagcggttgc ggcgaccgtt ctcggccacc agttgctcgt acggaaattc ggcttgcggg
    10921 tatttatata aagctttggc gtacgtatat gtcggcgttg cgtcgaggta gaagtagcat
    10981 tccttgacgt cctcgccgtg attgccttcg gggttcgtta atccgaacag gcgttctttc
    11041 aagattggat cgcggccatt ccatagcgct agtgcaaagc acaggcggca ttgccggtcg
    11101 gtgaagccca acagcccatc ctcgccccag cgataggccc ggctgcgggc gtgctcgtgc
    11161 gtgaaatagc gccacgggtt gccggcggcc gagtaatcct cgcgcgtggt accccattgg
    11221 cgctcggcca agtatgggcc ccagcgcttc caatccttgt cacggcgagc atcctccgcc
    11281 aggcgccgga cctcgatatc ccccgcagtc ttgcgtgcca taaaattgcc tttctgtact
    11341 tctgtatcgc ggggtcatcc actaatggta attatttgct taatgcccgg cgtgagcttg
    11401 accaggaact ccggggccgc ctcgattgga tggcgcccgg tgatcagcgc cggcacggtg
    11461 ttcggccagc ggctggtaaa gacgcccaag tcgcggatgg cggcttcgaa ggcgctgcgg
    11521 tcagcattga cggtgccgaa gacaatctgg ttcttcagca ccaggtcgcg catgatcagg
    11581 tttccctcga ccgaaatcgg gcccttgcgc cccggcacgc cggtgaacac gaagaccgca
    11641 ttcgtaccca ggaagggcat cgcgtcgaat gccagggttg acgcgcccgt ggcttcaaaa
    11701 atcaggtcaa tgttaccaat gcgtccgggc agagctttca gcggggtctc cggagccgct
    11761 atgaactctg caccgattga ctccagcaac tgcccgatgc gctgcgggtc aaacacgcgt
    11821 gaatacacac acgtcttgaa gcccgcccgg cggagcgtca gggcgcccag caagccgacc
    11881 ggccccgccc ccaagaccaa cgcctggtga caatagcccg gttcgccctg agccggctgc
    11941 gggcagcccc agggcaaccg ttgctgcacc agcagcacct gcgccagccc cttttccgcg
    12001 attgtcagcg gctcggtcag gacggccact tgccgcaatt cccgcggcac caggttcatg
    12061 tacttctgat catcgaccac gaactccgtc atgtacccgt gcagttcctt gatcccgcgc
    12121 tccttgtaac gcccagtaaa gcagaaatcc ggccgacccg cgcggcatgc catacaatgc
    12181 ggatcaggac aaggccggcg gaccattaag accaccaggt cgcccggccg gaagttctgc
    12241 acttgagcgc ctacttcaag cacttcgccc agcgactcgt gaccgatgat caggtagtct
    12301 tcgccgggag gcggtgtgcc gtatacaaaa gtggcgatgt cacggtcggt tccgcaaata
    12361 ccgacttcca gcatgcgcaa cttgacttca gttggtgcgg caatctgcgg ttcggggtgc
    12421 tcgatcagct tgatctcgcg cgtcgcaggg aagacggcca tggctttcat cgcagatacc
    12481 tcgttctcac atcagtattt gccacttcac gctccgcata cgtcggccgt ccggcccagg
    12541 cagtgttctt ccagggcgcg caccttcgcc agacgccgcg cgtgccgttc ggcgccgctg
    12601 aacgccgccc gcagaaacgt gaatacgagc tctgcggcca atgccggacc aatcacgcgg
    12661 gcccccaaag tcaatacatt catgtcatca tgctccacgc cctggtgcgc ggagtatgtg
    12721 tcgtgacata agccggcccg aatcccaacc agcttgttgg ccgcgacgct ggcgccgact
    12781 cccgaaccgc agatgagcac gccgcgttcg gcctggccgg tctgaatcgc ccggcccagt
    12841 gcggcggcaa aatcgggata gtcgtcgggc tgatccgcgc tgcgcgtacc cagatcgatc
    12901 gtttcatagc ccgcggtcgt taactcccgg cgtatgaatt ccttcaggtc aaacccggcg
    12961 tgatcggcgg cgatcgcgat acgcacgttc acctcgcgtg ttcccggccg aagcggccga
    13021 caaaagtaac cgcactgcaa accgcggccg ctagcggcat ttccagccgc cgaggcggcc
    13081 ctccgccagg cgctgcgcct ccggcggacc ccagctaccc gcggcgtagt tggggaactt
    13141 gggcggcggt cccgcggacc atgcttccag aatcggcgcg acgaatttcc aggacgcctc
    13201 cagttcatcc gagcgcggaa ataacgtggc atcgccgcgc agcgcatcca gcaacagacg
    13261 ctcgtaggcc tcgggcaact cccgtcccag ccgctgataa ttgaagtcga agcacaccgg
    13321 ctccaaatcc atcaacatgc ccggcctttt ggccgagaac gacaggctga tgccttcatt
    13381 cggctgaatg cggaactgta gcgtcgccgg ctggcccggg ctttcgatgc acaaatcccc
    13441 ttcgcactca acggtttgaa ataactgcag cggcggcagc ttgaactgca cctcaatctc
    13501 agtggcgcgc cgcgccaagc gtttgccggt gcgcaacaac aacggtacgc cggcccagcg
    13561 ccaactggcg atctcggccc gcaaggccac gaatgtttcg gtactcgaat tcgggcttac
    13621 gccttcttcc tcgcgataac cgcgcactgc ccgcccgtcg accacgcctg gcccatactg
    13681 tccgcgaacg acctgccgct caacttcggc gccggttggc ggaacaagcg tgcgcagaac
    13741 tttcagcttc gcgtcgctga tgtcgccggc cgtcagcgtg gccggtggat ccatggtcac
    13801 cagcgccagc aattgcagca ggtgattctg gagcatgtcg cgcagcgcac cggcccggtc
    13861 gtagtaagcc ccgcgccgcc cctccattcc ggccgtttca gacacagtga tttggatgaa
    13921 ctcgaccagt tcgcgccgga acagcggctc aaataccgaa ttgccgaacc ggaccgccaa
    13981 caggttctga accgtctcct tcccaaggta gtgatcgatg cggtacacct gatccggccg
    14041 caggaaggtt tcaatataac gatccaattc gcgtgcactg gccaggtcgt gcccgaaggg
    14101 cttctcgatc actacccgcg cccagggccg ctcgacgttg cggcgcacca agcccgcctc
    14161 gtccaacgcg gcgatcgtcg gggcaaagaa gtgcggcgcc acggccaaat agaagacgcg
    14221 gttgcccggc aaacgtgtcg cccgctccag cgcctggacg cgcccggcca gcgatcgcaa
    14281 accgtccgtg ccggccaagt cggcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnngga gctacacgcc gcagcgtagg ctcggccggt gtaaccgggg ctggcgtggg
    14461 acgaatgatg gtgccggttg acatgcagct actcctcgcc ggcgatcatt gcaccgcctc
    14521 gcgcgtcagt tccgtcaggg cttccagtcc cgccggcacg tccggcccca ggtgtacccg
    14581 caggatgcgc cggtcgcgtt cggccagtac ctggaaatcg ccccgcgcct gcgcggcttg
    14641 gaccgcaccg aacgtgtact tgtgccccgg cactggcaga tccgaggcct gctcatgcgt
    14701 tatcagcaag aacacacccg tgttcggccc gcccttgtaa gcttgtccgg tcgagtgcag
    14761 gtagcgcggc ccgaaaccgc cgcacgtcgc cacacgcctg gcattccgca ccagccgtcg
    14821 cattgtgccc agttgctcgc tgttgaacgc attgcgctcg acatacgcca gcagtgcgaa
    14881 ataatccccg ctgcccagcc gcttcaaatg ggccttcaac aagccgcgcg gcgagcgatc
    14941 cgtccccgcg gcgtacatca gggccgacgc gttcttttcg tcggcgaaga agctcaggcc
    15001 gtctccgcgc agaatcggtt cttcccgcgg caacgccccg tcttcctcga acgccgccgt
    15061 cagcttccgc gcctgcaact tggcctcttc cacgtccggc tgattaaacg ggttaacgcc
    15121 gagcaccgcg ctcgcgacgg ccgtcgcaat ctgccagcgg aagaactccg cccccagaca
    15181 gtacgcgtcc tgcattgtga tgcgaacgac cggatgtccc gcccgctgca gggcgtcgac
    15241 cgccgcgtcc tgcccgttgt ccggcgtacc gctcagccgc aaatacacga acaggcgatc
    15301 agtgtcgtac acctccggcg gcccgagcgg ttcgcgatcc acaggaatca cgccgtcgcc
    15361 atccttgccg gtcgattcgg ccagaagctg ttcaagccag gcccccagcc ccgccacgcc
    15421 cggtgaggcg ataacggtga ccttgtcgcg cccattgcgc gccagcacgc ccatgatcac
    15481 gcccagcacc acgcccgggt tttcctcagc cggcacgcac gaccggcacg cttgcgccat
    15541 ctcttctgcc gtatccagca gccgcggaac atccaacccc atgatcgccg ccggaatcag
    15601 cccgaagttc gacagcgccg aataccgtcc gccgatgccg ggcacgccgt ggaacaagcg
    15661 gcggaaggca ttgccctccg caacgcgctc caacttcgag cccggatcgg taatcgcgat
    15721 aaaacggcgc cccgcctcgg cctccccgac cgccgcgcgt acgcgctcga agaagtagtc
    15781 tttgagaatg tttggttcga gcgtcgtgcc ggacttgctc gagacgatga acaacgtcct
    15841 tgcaaaatca atttgcctct caaccgctcg gatctcatcc ggatcggtcg aatccaacac
    15901 cagcagcacc gggtgaccgg cctgcggccc gaaagtctgc gccagaacct ccggcgccag
    15961 actcgagccg cccatgccca gcagcacggc gcgcgtaaag ccggccgatt ggatttcttc
    16021 ggcgacttcc tcaaaacgtg ctccctccgc cggctggtct tccgtcacgg ccagccagtc
    16081 catccagtgc ccctcatcac ggcctgtcca gagccccgcg tcgcgcgccc acaaccgccg
    16141 aaccttgcct tccgcatgcc agctttgcag cgtgctctcg acgtcctccg ccagcggctg
    16201 cggcaagtgg taactcatgc gcttcatcgc cggccgcccc gttcccttga tcttctcgcg
    16261 aatggccgag atcaaccgct cgaacgactc gctgaagagc tgcacgccgt catcgagcac
    16321 cttctgcgta acttcgggca gcgatatgcc cacctgcgcc agggtttcca ggacctcgcg
    16381 cgcctcctcg acgtcctctt ccagactggg ccgcacgcgc ccgtgatcgc gaaacgcgtc
    16441 catggtcgcg ggcggcatgg tgttgatggt gtccggcccg atcaattcct cgacgtagag
    16501 cacgtcgcgg tagctcgggt tcttcgtgct cgtgctggcc catagcagcc gctggggcat
    16561 ggcgcctttg cgagccagtt tctgccagcg ctggctgcgc acgatttcct tgtaagtctg
    16621 atatgccagc ttcgcgttgg ctatcgccac cttgcctaaa agccagcgca gcttggaatg
    16681 gtcttcgccg cccgccgccc tcagccgctc tgtgagaagt gcgtccacgg cggtatcgat
    16741 gcggctgacg aaaaagctcg ccacactcgc cacacgtgcc acgtcgccgc cgcgggccgc
    16801 gaactgctcc aacccggcga ggtaggcctc cgcgacctgt tcgtacatct cgcgcgcgaa
    16861 cagcagcgtg atgttgacgt tgatcccctc ggatatgagc tgccgaatcg cgggcacgcc
    16921 ctcttttgtt ccggggactt taatgaatac gttgtcccac ccgacttcgc gccatagccg
    16981 ccgcgcctgt tccaaggtgc tctgcgtgtc gtgcgccacc aacggcgaga cttccagact
    17041 cacgtagccg tcgcgccgcc gcagttgctc gtacagcggt cgaaacacct cggccgcccc
    17101 ttggatgtcg cgcaccgcca gcagttcgta aacgagcagc gggtcgcgtt ccccacgccg
    17161 ggccgcggtc gacagaatgt cgtcataatc cgtgctgccg gtaaccgcct tctcgaagat
    17221 cgaagggttt gaggtgactc cgcataagcc gtcttcttcc cccaggcggg ccagctcacc
    17281 gctggttatc aaatgccggc ggatgtagtc cagccagatg gattgcccgt atcggcgcag
    17341 ttccagcaat ggattagcca ccggcgttcg ggccggcagt gactcggtaa tgacttgcat
    17401 gactacggtc tcccgcgagg tcctgaacct cgccttaggc tctgcacgtc agcatctgtg
    17461 ccgcctcctc atccaccatc catgtgagcg agccgcgttg cggcttcacg cccgccgccg
    17521 ggcgctgcgc gggacgccgc ctttcctcca acacatcccg cagcacggcc gccttcttcg
    17581 ctccacagac caggaacagc acgctgcggc tcgcattgag aaccggataa gtgaacgtga
    17641 tccggtcgac gggcggcggc agcttgcccg gtggactcca tgtgacccac tcgttggtaa
    17701 tcctcagcac gggcgcgtgc ggaaagagtc cggccgtgtg cccgtcttcg cctaatccga
    17761 gcagaatcag gtcgaaacgc ggcggtgcgt gctcgcaacc ggtgaagtgc tgggccagtt
    17821 cctccgaata ggccgcggcc gcttccgccg cactgccgag cgtggtcggc accgagtgta
    17881 cctggctggc cggaataggt acgcccgcca acagcgttcg tcgcacagtg gcgaagttgc
    17941 tgcgctcgtc atcgggcggc acgaaccgct catcaccgaa gaacagatgc gtgttctccc
    18001 acggcatcgc gctaacctgc tgcggttcgg ccagatgccg ataggtttcc tccggggtct
    18061 tgccacccgc cagcgccagc ataaaacgcc cgcgctcctg aaccgcctcg cgcgcagcgc
    18121 gcgcgatgaa ttcggccgcc tgcgccgcca aggcagtgcc attatcgagg acaatcaggg
    18181 acgatggata catatagttc caatggttcc cagttccaac gctccctatt aaggccgcga
    18241 ggtaaatcgc aggtgaattt cgcctgagcc cccgttcatc atgctgttga tcgtgcttgg
    18301 ataggtagcg gcgggactac gtgcgcggct caatctgccg tacaacttct cggagccgcg
    18361 ttgtatatga gggcagcggc gtcagcccgc tggtttgaag ctctgcggcg atcgcgcgga
    18421 cgctgcggcc cgaatccggc aataccagcg tcggcgccag ctcgcacaaa cccgccgcca
    18481 ggaacgcccg cgtaagaata tccggatcgg gaactttcaa ttccgcagaa tgcagcacaa
    18541 ggtcatcgta aacgatcagg tccagatcaa tgcagcgggc cgcgtaccga tcatcggtgc
    18601 gccgtcgccc gagctcgcgc tcaatctcgt gaagcaactg gctcaattcc agcggcggca
    18661 atgccgtctc gaccgctaca acgcagttgt aatagtcagc ctgctccggc cgtccgatgg
    18721 gggcggtgcg atagacagtc gacacgcctg cgactctgac atgctgcgcg agccgctgta
    18781 tgccgagcaa cacgttccgc tcggggtcaa cgtttgaacc cacgccgatg aaagcccgcg
    18841 ccatcattca ccacgccggc gcttgatgtc gaccgccacg ctgcgcgcaa agcgcaatgc
    18901 ccccggctta tcaaccgaca ctcgcacggc gtgcacggcc ggatattcca ggcacagccg
    18961 cgcgatgtcc tcggccagcg cctccaggag ccgccggtgt gagttctcca ccagggccac
    19021 gaccctcttt ttcaaagcgc tgtagtccag ggcgtcggcg aaatcatcgc tgcgccccgg
    19081 ccgctgcaaa tccgtgtaga tgacaagatt gataattacg tcctgcttct ggcgacgctc
    19141 cgcatcttcc acgccgatga tgcagcgcac gctcaggtcg cgaatgcgta tgcgatccac
    19201 ggactgccct cactcatgtt gagtttccag tcatctcata ggcgttccgg acagcgctcg
    19261 tccgctagcc tgcggcgcgc tattatcgcg agtgaatgcc acgatgcaca accgatcacc
    19321 cgcagaatgt tctaatgtcc tcggctgcgt ccaaactcag cactgaagag cttccgcccg
    19381 gggcggccgc tacgagccgc cgcctttcag agcagcaggc acgaactttt gagggttaca
    19441 tcgcggaaat cctggccgct tgcggtctga atctggaact tccgcccacg gccgacacgc
    19501 cgcgccgctt cctccaggcg ctgctcgacg ccactgaggg ctatgaaggc gatccaaaac
    19561 tcgtccgggt ctttgacacc gagtgccgcg gcggatctga ctgccggctg agccagataa
    19621 tcgaagggcc gatccccttc gcctcgcttt gcgagcacca tgcgttcccg ttctatggtc
    19681 gagtatacat cgggtatatc ccgcacgaac acatatttgg gctttcgaag ctcgtccgcc
    19741 tggtgcgttt gtacacgcgc cgttttgcgg tccaggagcg cattggccgg caaatcgcag
    19801 atacgatggt ggaaatcctg caaccgcatg cggtggctgt ttacgtgacc gcacggcata
    19861 tgtgcaccca gatgcgcggc gtccgcgctg tggctccaca tacacgcacg acggtctggc
    19921 ggggtgaata tgagcgcaac cgttcactgc gagccgagtt cctggcggcc tgcggagtgg
    19981 gacagcggac ggcatgaccg gccggacagg accacggctg gagcataggt atcccggagc
    20041 atatccatga tccgtaccca acgagtttac gaaaaacagg gcaacggcgg ggcgcactac
    20101 cttgtcgagc gcctctggcc gcgcggcgtt cgcaaggaag cgctgcgctt ggacggctgg
    20161 ttgcgtgaag cggcgcccag caccgaat
//
LOCUS       DAOSYS010000038        19316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2327_length_19316_cov_5.459166, whole genome shotgun sequence.
ACCESSION   DAOSYS010000038 DAOSYS010000000
VERSION     DAOSYS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19316
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_19316_cov_5.459166"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..152
                     /locus_tag="PLU99_03880"
     CDS             12..152
                     /locus_tag="PLU99_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOO16227.1"
                     /translation="MGAGSQLPANVNLITGPSKTADIEGILVTGVHGPREVHVILLQN
                     AE"
     gene            complement(248..1321)
                     /locus_tag="PLU99_03885"
     CDS             complement(248..1321)
                     /locus_tag="PLU99_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOO16228.1"
                     /translation="MDNKMLRPIVKRARRSDRLLQVLSEYERFVIVTHDCPDPDAIAS
                     ACALAPLLEYRLKRPARIVAGGAVMRGENLRMLELLKLPIDFVDELDVSDPRVAVILV
                     DSVPTAVNHLLANSGVEPVAVIDHHRPQGRPYRVRFRDIRPNLAATATIVTLYLREQN
                     LPPTPLVATALLYGIRTDAVGLPRMSVVDQRAAVWLMRFADFSKLGEIENAPLSREYF
                     SDLLLAMKNTFVYDDAALCFLPRAAGPEIVGEVADLLIRCRDINRLMCAAVLGGDVLF
                     SVRTTAAGGDATELMRKAIEGVGYGGGHRRRAGGKLIVAARSDRISEDLQTELRTRWL
                     KACGIEFQRGTRLVTRREILEDL"
     gene            complement(1418..2956)
                     /locus_tag="PLU99_03890"
     CDS             complement(1418..2956)
                     /locus_tag="PLU99_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOO16229.1"
                     /translation="MKRPVTSLVLGRTLIAVVLAVVFGAPAGAAAYASKAAPSAVPGA
                     PRAGPRGPAGQEQAPSQDGPADLSAAFERVAQIIQPSVVNVESVKRVQVSQRAPRELR
                     EFFGGDLFEKLFPERRDTQEGLGSGVIISPEGYILTNAHVVQNAERVRVTLPAPDDRE
                     FVADVVGTDPLTDLAVIKIAANGLRPAKLGNSDELRVGEWVVAVGNPFGLKSTMTAGI
                     VSATGRSNVGVASYEDLIQTDAAINPGNSGGPLVNLRGEVVGITTAIFSRSGGYMGIG
                     FAIPSNMVEKIMPQLIAEGRVIRGSLGVAIQNLNPGLARTFGYDSASGVLISDVTPSS
                     PAEKAGLQRGDIVVSYRGQPTAKSADLRGMVADTKPGTRVPLEIVRNGKPMSLEVTVG
                     ELAEKVQTASHEEKTPSAGSQTKMGITIHDLTPELIRELRLGPEQRGVVVTKVEPLSP
                     ADRAGLSVGDVITVVGDRPVKNIDDFKEAMSGQDLSRGVRLEVVSSTRREGPLRRYAF
                     VQTD"
     gene            3168..3386
                     /locus_tag="PLU99_03895"
     CDS             3168..3386
                     /locus_tag="PLU99_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOO16230.1"
                     /translation="MNLGKLLVIAGLTLAIIGALIWGFGRLGIRRLPGDIYYQSDGVR
                     IYIPIVTCLVLSILLTAVIWVWQWLGRR"
     gene            complement(3333..5348)
                     /gene="ftsH"
                     /locus_tag="PLU99_03900"
     CDS             complement(3333..5348)
                     /gene="ftsH"
                     /locus_tag="PLU99_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOO16231.1"
                     /translation="MPPYEAPPEPHRPRPRSRWQRWQFLFWYLLLALWLLFLWQSSFG
                     RAEVRQISYSEFKEFLAKGEVVEAAIGQNEIRGVIELKSGTTQPTAEKLPDKFNFRTV
                     RVEDPKLTDELHAAGVHFKGVLPSKVGEFIWAWIFPLGLIVLLWFGLSRFLRGAGSQV
                     MTIGKSRARIVPDRSTGVTFNDVAGADEAKAELKEVVDFLKQPQHYTVMGAQIPKGIL
                     LTGPPGTGKTLLARAVAGEAHVPFFSISGSDFVEMFVGVGAARVRDLFAQAKQKAPCI
                     VFIDELDAIGRQRGFHVGAVNDEREQTLNQLLVEMDGFEANTGVIILAATNRPDVLDR
                     ALLRPGRFDRQVIVDAPDLNGREAILRVHARGKPLDSEVNLRRIAQATPGFSGADLAN
                     ALNEAALLAVRKGHQRIMQSDLEEAVEKVVAGPERKSRRLNDAEKRRVAYHEVGHAIV
                     AAYSRHTDPVHKISIVPRGRAALGYTLQMPTEDQFLMTREELIDRITGLLGGRAAEEV
                     IFNTISTGAQSDLERATTLARQMVCLYGMSERLGLIQCAVRDGRLPGEDGLMQRDCSE
                     ETAREVDEEVRRILDECHQKARSLLTEHRDELERIAGELLQRETIDRQTFERLLDQEK
                     FAEMKREKSPDARPIPLPAHSGGDEFSEPESASAQPLPDPNDRRQQN"
     gene            complement(5348..6316)
                     /locus_tag="PLU99_03905"
     CDS             complement(5348..6316)
                     /locus_tag="PLU99_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOO16232.1"
                     /translation="MAGDASQIVRRDELAAPVTLVSIERRIRDALIRTLPQLIQECGA
                     AAALVYAEALDAQELELPPEIASRLLYVTKTRRQEEQHEERGHRILRVPNVRLSRMGQ
                     AQVAILLAVSRKLIEPDEVVVVLTGMPGGSSLDTLLVLHVRSELDMPLDAATESDLSR
                     DVRPDVLERVLNIAAELGYEGREGKMVGGLFVVGDTQNVLALSRQLILNPFKGYPEEA
                     RNILAPELKETVKELSSIDGAFIIRGDGVIEAAGTYLTASTRDIHDLPAGLGARHQAA
                     AGITAVTNAVAIAVSQSTGHVTVFRRGHIIADLEKPRTRTEPGEGD"
     gene            6507..8258
                     /locus_tag="PLU99_03910"
     CDS             6507..8258
                     /locus_tag="PLU99_03910"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOO16233.1"
                     /translation="MSNVGLLGGLPTEFPRAYVAKETEFADWPTAESYYRELLGRNVG
                     SAAQLERWLYDASELEACLDEERCARYVEATRQTDDESRQQRYIQFVEQVEARQKPWQ
                     DKLLRRLLDLADQFPLPPLRYEVLLRRARNRTELYREENIPLLTEEAKLVSEYQKITG
                     AMTCRYDGREQTLQQMARYQEETDRSVREEAWRLMADRFQQDAERLDELFVKMTVLRD
                     RIARNAGCADYRDYSFKNYGRFDYTPADCLAFHDAIERCVVPAARELAEQRRRKLGLK
                     ALRPWDFNVDPAGRPPLRPFENVDQLVQGCSRIFQRVNPELGRIFDKLAALGVLDLDS
                     RKGKAPGGYQETFAEHRLPFIFMNAVGTDNDVRTLLHEGGHAFHSWACRNEPLLPYRS
                     ADGAIEFAEVASMGMECLALPHVEIFFGPETQRANKRYFERIVQFFPYMARVDAFQHY
                     VYTHLDAGLSGWKDHWQALTRRFSPEVDWSGLEAYDSRSWQRKLHIYEVPFYYVEYGI
                     AQLGALQVWRNSLRDYAHAVALYQNGLALGGSRPLPELFEAAGCQFDFTEATLRPLIE
                     AVMAETGAVAPGAVPKL"
     gene            8654..9862
                     /locus_tag="PLU99_03915"
     CDS             8654..9862
                     /locus_tag="PLU99_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO16234.1"
                     /translation="MTLETATEKEKSGFVPQRPHELSEVHPRQARRPWLRKLIPAALI
                     VLALAGVYCIAMLPKAADDAPPIEIPPVNVSVMTVTAVPEMPDSVTLSAVVEPDYVVR
                     VAAEVAGRIEKWGQRRSDAYWLGRHYPAGTTVLEGEPVTEGDEILYLNTDLLKARFDR
                     AVAQYEFDEREWRRILNLSETGATTTNELADARTKRDMSKAALDEVTRQLERAVIVAP
                     ISGVLNKQCMKIGEYASPGERVAEIVKLDIMKVVVDVPERDAPYLKIGDLATIFTGPP
                     REAELTGKISYISELAHAGSRTTRVEIAVENRNQLLRSGQIVRTRLTRRMLQNVIMIP
                     LGAVIPLEDGKLVYIVKDGKAERRDVQLGFIRAREVQAVSGLEPGDLLIVEGHRYVGP
                     GQAVRVVGEY"
     gene            10049..13180
                     /locus_tag="PLU99_03920"
     CDS             10049..13180
                     /locus_tag="PLU99_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO16235.1"
                     /translation="MILSDLAVKNRTTVWVFSLLVVVFGLYSYFTLPREASPDVPIPI
                     ILVSTIYEGVAPQDIESAVTIKLERQLAGLKGVKEISSHSAEGISIIVIEFLPNVKID
                     DAMQYVRDKIDLAKKDLPQEAEETTLTEINVAEFPILMVNISGDDSPWRQRLLADRLE
                     DEIEAIPGVLNCDVLGALEREIRLEIDQDKVAAYGLTIPEILKLIPSENVNISAGGLD
                     TPGTKFNVRVPAEFVEPEEVDELIIAERDGHPIYLTDVAAVHDTFKDRVAFSRMNGKE
                     SITVSVQKRLGENIIDITDAVKRVLAEFQRRAPAGMRFEITLDQSKDIRSMVADLENN
                     LISGMILVVAVMVIFMGWRPSLIVALEIPMSMLLTYTTLQLLGYTLNMIVLFSLIMAV
                     GMVVDNAIVMVENIYRHYQLSGRRIQAAMTGAGEVAWPVITSTLTTVAAFAPLIFWPG
                     IMGGFMKYLPITLIIALLASLLIALVVNPMLSSIFVTREKTHGPSRFMHGYRRLLATA
                     LEHRFTTLCLAVLLLVGLVILYGKQGHGVELFPEFDPRRAIIDIRCPQGTNIRETDRL
                     ARTIEQRLEPFAADLERVITNVGHSGGGEGNLGGSAAGEHLASIVLTFHDYEIRRRPS
                     TEVIAEIRQAIADLPGAEIKVQKQEEGPPTGAAVTVRLVGEDFKVLENLSEQARNLIA
                     NVPGLVNLRSDHEAARPELAFRVDRRRAMLLGVNTATVGNFLKTAVFGSEVGKYRQYN
                     DEYDITVRLPLSQRQSIDDLFRLRVPNANGEAVPLSSLGDFEYTGGYGIISRINQKRV
                     ITLMGDAEGRLDTAVLADVQERLKELDLPLGYEVRYAGQKEEQDEAQAFLTKAFFIAL
                     FLILMILVTQFNTLIVPLIIMTTVILSLIGVFAGLLICKMPFGIIMTGIGVISLAGVV
                     VNNAIVLLDYTRQLQRRGIDLIAASIEAGATRLRPVLLTACTTILGLIPMALGMSYDF
                     HKFEWAWKSESSQWWASMAIAVIFGLAFATILTLVVVPCLYVTLTRLTTRLGLGQAAE
                     EAEIPDDACEVIR"
     gene            complement(13247..>14241)
                     /gene="cyoE"
                     /locus_tag="PLU99_03925"
     CDS             complement(13247..>14241)
                     /gene="cyoE"
                     /locus_tag="PLU99_03925"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOO16236.1"
                     /translation="GRKHRLPAPPATLDFADRAIEPDGRAVEPSDRTVAVTHRAAVAG
                     QTRHLLSLYAELAKVRVCAMVLLTTLGGFVLASGDIDWKRLLWTLLGTALTACGSAAL
                     NQCSEVSRDSRMTRTCRRPLPSGALSLGAARVFGLASGVAGWALLWWTVNGLSAWLAA
                     VNLVIYVGLYTPLKPVTPLSTLVGGLVGALPPIIGWTGATGRIEVGALTLGGLLFIWQ
                     IAHFLSLAWVYREDYARAGFRMLPVADRSGRVTCLMVAACCALLIPAAYGMVLAGVCG
                     PAYAVGSVVLGGALLLVSVGWGRARSVSGARRVFLASNLYLPLLLGLMMADRAG"
     assembly_gap    14242..14341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14342..14898
                     /locus_tag="PLU99_03930"
     CDS             <14342..14898
                     /locus_tag="PLU99_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO16237.1"
                     /translation="WMAWRTTCIVHGMDGRIILETERVVLREVTLADLNFVADMVGNR
                     EVMRFWPRLLSRDEARQGLQKHLADYQRYGHGYWLAVNRQTGEPIGQAGLLMSEVEGV
                     LHPALGYMLHRPFWGQGYATECGAAVLKWAFEQRDYQRVICLIRPENIPSLRVAIRLG
                     LRPESYVVFKGFNHLVFVARRTGP"
     gene            15062..15466
                     /gene="atpC"
                     /locus_tag="PLU99_03935"
     CDS             15062..15466
                     /gene="atpC"
                     /locus_tag="PLU99_03935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOO16238.1"
                     /translation="MATKAPIRLVVVTPERQVVSEQTASVVFAAHDGELGVLHNRAPL
                     MCELGIGQLRYEAGGQTRRCFVDGGFAQVREDTVTILAHQAIPAEQVTPEMVGEAERK
                     LEELRGTGPEAQEERSKAQQRASALRRILANE"
     gene            complement(15565..16353)
                     /locus_tag="PLU99_03940"
     CDS             complement(15565..16353)
                     /locus_tag="PLU99_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16239.1"
                     /translation="MNPEIKDSPVAESADVDSQIVQVRDLVKVYATSGRPVTALNGVS
                     FTVPAGCFIAIMGASGSGKSTLLHLIGGLTQPTSGEVLVENVNLGALSDRQRTLFRRR
                     RLGIIFQEYNLLPTLSARENVALPLLVDGQNPDQFQTRIDDLLRAVHLEERADHRPDA
                     LSGGEQQRVAIARALLNDPAIVLADEPTGNLDSRQSVEIWQLMRRIVLEQGKTILMVT
                     HEALGAAYADRVLILKDGRLVGMLEPRGSGDAGPVAAGYQQLAG"
     gene            complement(16452..17894)
                     /gene="lpdA"
                     /locus_tag="PLU99_03945"
     CDS             complement(16452..17894)
                     /gene="lpdA"
                     /locus_tag="PLU99_03945"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOO16240.1"
                     /translation="MKQHDLVVIGSGPGGYVAAIRAAQLGLNVACVEMETALGGTCLR
                     VGCIPSKALLESSERFAAARHSLAEHGVKVAGVELDLSAMLNRKEKIVSGLTKGIEAL
                     FKKNKVTRYAGRGRIAGPGRVVVEPLAPPQLAGEGGVPSVSPAPGGGLEEISAKNIII
                     ATGSRSAELPGVKPDGQRIGTSTEALSYPEVPKHLVVIGAGYIGLELGTVWARLGAKV
                     TVLEYLDHILPNTDSEIAAEALKLFKRQGMEFRLGSRVTGARTVGEKCIVEAEGAAPL
                     ECDRVLLSVGRVPNTEGLGLESVGIKLDEKRRIPVDDNFATTAPGIYAIGDVIRGPML
                     AHKASEEGIACVDRLVTGFGEVNYDAIPAVCYTEPEVAGVGKTEEQLKQLGTEYKKGV
                     FQFRGNGRARALGQVDGRVKILADAKTDRVLGVHIIGPRAGDLIAEAAVAIAFGASAE
                     DLGRCSHAHPTLAEALKEAALAVSGREIHA"
     gene            18042..>19316
                     /gene="dnaX"
                     /locus_tag="PLU99_03950"
     CDS             18042..>19316
                     /gene="dnaX"
                     /locus_tag="PLU99_03950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOO16241.1"
                     /translation="MAYTVLARKYRSRTFEDVIGQQPIATTLVNAIKSGRIHHGYLFT
                     GTRGVGKTSMARILAKALNCLKAEAPTPTPCGECDVCRAIAEGQDIDVIEIDAASNTG
                     VDNIRELRSNTAYRPARARYKIYIIDEVHMLSTGAFNALLKTLEEPPEHVKFILATTE
                     IQKVPATIQSRCQRFVFRNIGPADIEKRLAEIVRAEGAKAEEAVLRRIARLANGSMRD
                     ALSILDQLLSVSPTKLTAELLDEILPPAQDEQIFGLLKDAAAGDAAAVLADVDKILAG
                     GRGLDVFCNDTIEAVRALMLVRVCGVNAPMVDLPAGTQEEYVRLSGQFELSQYVQMIS
                     MLEELRRNVRFSGAGRALTDACMVRLARMRQWAPIERLLAELPESADGSEKKKPPVSE
                     RAVAADSRAQTANSSAPGTSAYGAGVGPVSQQR"
BASE COUNT         3543 a         6383 c         5953 g         3337 t
ORIGIN      
        1 gcctggccct aatcggcgcc ggctcgcagc ttccggccaa cgtcaacctg atcaccggcc
       61 ccagcaagac cgctgacatt gagggcatat tggtgaccgg cgtacatggc ccgcgtgaag
      121 tccacgtcat tctgctgcag aatgcagaat gaaaaatgca gaattctgca caatgacaaa
      181 gacaagatga ccggcgtcag ccgttcactt catcctgcat ttgtcacttc gcgttcgcca
      241 tctgttttca caggtcctcg agaatttcgc gccgcgttac caggcgcgtg ccgcgttgga
      301 attcgatgcc gcaggctttc agccagcggg tgcgcagttc ggtctgcaga tcttcgctga
      361 tgcggtcgga gcgggctgcc acaatcaact tgccgcccgc gcgccgccgg tgtccgccgc
      421 cgtagcccac gccttcgatg gctttacgca tgagctcggt cgcgtccccg cccgccgcgg
      481 tggtgcgcac tgagaaaagc acgtcgccgc ccaagaccgc ggcacacatg aggcggttaa
      541 tgtcgcggca gcgaatcagc agatccgcga cctcgcccac gatctccgga ccggcggcgc
      601 gcggcaagaa acacagtgcc gcatcgtcat agacaaacgt gttcttcatc gccagcagca
      661 gatcggagaa atactcgcgc gacagcgggg cgttctcaat ttcgcccagc ttggaaaagt
      721 ccgcgaaccg catcagccag acggccgccc gctgatcgac gactgacatg cgcggcagtc
      781 cgaccgcatc cgtgcgaatg ccgtacagca acgcggtcgc gaccagcggc gtcggcggca
      841 ggttttgctc gcgcaggtag agcgtcacga tagtggccgt cgccgccaga ttcgggcgaa
      901 tatcgcgaaa acgcacgcgg tacggccgcc cctgcggccg atggtggtcg atgaccgcca
      961 ccggttcgac cccggaattg gccaacaggt ggttcacggc ggtcggcacc gagtcaacca
     1021 gaatgaccgc cacgcgcgga tcgctcacgt cgagctcgtc cacgaaatca atcggcagct
     1081 tcaacagctc cagcatgcgc aaattctcgc cgcgcatcac cgccccgccg gccacgatgc
     1141 gcgccgggcg cttcaggcgg tattccagca gcggcgccaa agcgcaggcc gaggcgatgg
     1201 cgtccggatc gggacagtcg tgcgtgacga tcacaaagcg ttcgtactcg ctcagcactt
     1261 gcagcaggcg gtccgaacgg cgagccctct ttacaatcgg ccgcagcatc ttgttatcca
     1321 tgccagaggt atccagaagc ggactatacg caacggggcc gcgcccgcaa ccgtgccgcc
     1381 aggcgcacgg ctggccgccc gccgccgcgt ttgggcgtta gtcagtctga acgaaggcat
     1441 agcgtcgcag cgggccttcg cgccgcgtgc tcgagacgac ctccagccgg acgccccggc
     1501 tcaggtcctg cccgctcatc gcctccttga aatcatcgat attcttcacc ggacggtccc
     1561 caaccaccgt aatcacgtca ccgacgctca gaccggcgcg atccgccggg ctcaagggct
     1621 cgaccttggt gaccaccacg ccgcgctgct ccggcccgag gcgcagctct cttatcagct
     1681 ccggcgtcag gtcgtgtatg gtaatcccca tcttggtctg gctgccggcg gagggcgtct
     1741 tttcctcgtg actggccgtc tggacctttt cggccaactc gcccaccgtt acttccagcg
     1801 acatcggctt gccgttgcgg acgatttcca gcggtacgcg cgtcccaggc ttcgtgtcgg
     1861 ccaccatgcc gcgcaggtcg gcgctcttgg ccgtcggttg cccgcggtag ctgaccacga
     1921 tgtcgccgcg ctgcaggccc gctttttcgg ccggcgagct aggagtaacg tcggagatca
     1981 gcacgccgct ggcgctgtcg tagccgaagg tgcgggccag cccggggttg aggttctgaa
     2041 tcgcaacgcc cagcgagccg cgaatcacgc ggccttcggc gattagctgc ggcatgatct
     2101 tttcaaccat gttggagggg atggcgaagc cgatacccat gtagccgccg ctacggctga
     2161 agatggcggt ggtaatccca accacttcgc cgcgcagatt caccagcggg ccgcccgagt
     2221 tgccgggatt gatagcggcg tcggtctgaa tgagatcttc gtagctggcg acgccgacgt
     2281 tcgagcggcc ggtggcgctg acgatgccgg ccgtcatggt tgacttaagt ccgaaaggat
     2341 ttcccaccgc gaccacccat tcgccgacac gcaactcgtc cgagttgccc agctttgcgg
     2401 ggcgcaaacc gttggcggcg attttgatga cggccaggtc ggtcagcggg tcagtgccga
     2461 cgacgtcggc gacaaactcg cggtcatccg gggcgggcag cgtcacgcgc acccgctcgg
     2521 cgttctgcac cacgtgggcg ttggtcagga tgtaaccttc ggggctgatg atcactccgc
     2581 tgccgagacc ttcctgcgta tcgcgacgct ccggaaagag cttctcaaac aggtcgccgc
     2641 cgaagaactc gcgcaactcg cgcggcgccc gctgcgaaac ctggacgcgt ttcacggact
     2701 ccacattgac caccgagggc tggataatct gggccacccg ttcgaaggcg gccgacaagt
     2761 ctgccgggcc atcctggctt ggggcttgct cttgacccgc aggcccgcgc ggtccggctc
     2821 gcggcgcgcc aggaacagcc gacggcgcgg ctttagaggc gtacgccgca gccccggccg
     2881 gggcgccgaa caccacagcc aggacaactg ctatcaaagt acggcccaag acgagcgaag
     2941 tcactgggcg tttcattcgt caactccttt ctggagctca tggcatgcga ctgcaacttt
     3001 tctttaggat agtagccgag ggccacaggg ctgctgctca acggccgggt gtgctgacag
     3061 catgatgatt cgcgcggcgg cagcccggac taatattgtg tgatgcgaac cggcacggac
     3121 gaaccccggc cgcgctacgc ccgagaaggc aaatgcgttg agaggcgatg aatctcggta
     3181 aactattggt aatagcgggc ctgacactgg ccattatcgg ggcgctcatc tggggatttg
     3241 gccggctcgg catccggcga ctgccggggg acatctacta tcaaagcgac ggcgtccgca
     3301 tctatatccc catcgtcacg tgcctggtgc tatcaattct gctgacggcg gtcatttggg
     3361 tctggcagtg gctgggccga cgctgattca ggctcggaga actcatcgcc gccggagtgc
     3421 gccggaagcg gaataggccg cgcatcgggc gacttctccc gtttcatctc ggcgaatttc
     3481 tcctggtcga gcagacgttc aaatgtctgc cggtcgatcg tctcgcgctg caacaactcg
     3541 ccggcgatcc gctccagctc gtcgcggtgc tcggtgagca gggaccgggc cttctggtga
     3601 cactcatcca ggatgcggcg cacctcctca tccacttcgc gggccgtctc ctcgctgcag
     3661 tcgcgttgca tcagcccgtc ttctccggga aggcgcccat cccgaaccgc gcactggata
     3721 agccccagac gctcgctcat cccatacaaa cagaccattt gccgcgccag cgtcgtggcg
     3781 cgttccaggt cactctgggc gccggtcgaa atggtattga aaatcacctc ctccgccgca
     3841 cgccccccca gcagccccgt gatccggtcg atcaactcct cgcgcgtcat caggaactgg
     3901 tcctccgtcg gcatctgcag ggtgtagccg agcgcggccc gcccgcgcgg cacgatgctg
     3961 atcttgtgta ccgggtcggt gtggcgcgag taggccgcaa cgatcgcgtg cccgacctcg
     4021 tgatacgcga cgcgccgttt ctcggcatcg ttcaggcggc ggcttttgcg ttccgggccg
     4081 gccacgactt tctccacggc ctcctccagg tccgactgca tgatgcgttg atggccctta
     4141 cgcaccgcca gcaaggccgc ctcgttcagc gcattggcca ggtcggcccc ggagaagccc
     4201 ggcgtggcct gtgcgatgcg ccgcaggttc acctcggaat ccagcggctt gccgcgcgcg
     4261 tgaacgcgca ggatcgcttc gcgtccgttc aagtccggcg catccacgat cacctgccgg
     4321 tcaaagcgtc ccggccgcaa cagcgcccga tcgaggacgt ccggccggtt ggtggcagcc
     4381 aggatgatca cgccggtatt ggcctcgaag ccgtccattt cgaccagtag ctgattcagc
     4441 gtctgttcgc gttcgtcgtt gaccgcgccc acgtgaaagc cgcgctgccg cccgatggca
     4501 tcgagctcgt caatgaaaac tatgcacggc gccttctgct tggcttgcgc gaacaggtcc
     4561 cgcacgcggg ccgcccccac gcccacgaac atctcaacga agtcactgcc gctgatcgag
     4621 aagaacggca cgtgcgcctc gcccgccacc gctcgcgcca gcagcgtctt tcccgtcccc
     4681 ggcggcccgg tcagcagaat gcccttcgga atctgcgccc ccatgacggt gtagtgttgc
     4741 ggctgcttca gaaaatcgac cacttccttg agctcggctt tggcctcgtc cgccccggcc
     4801 acgtcgttga acgtcacgcc ggtggaccgg tcaggaacga tgcgcgcccg gctcttgccg
     4861 atggtcatga cctggctgcc ggcgccccgc aagaaccgcg acaggccaaa ccatagcagc
     4921 actatcagcc ccagcgggaa tatccaggcc caaatgaact cgccgacctt gctgggcagg
     4981 acgcctttga agtgcactcc ggctgcgtgc agttcgtccg tcaacttcgg gtcttcaacc
     5041 cggacggtgc ggaaattgaa cttatcgggc aacttctccg ccgtgggctg cgtggtcccg
     5101 ctcttgagct caattactcc gcgaatctcg ttctgcccga tcgcggcctc gaccacctca
     5161 cccttggcca gaaactcctt aaattcactg taggagatct gccgcacctc ggcccggccg
     5221 aacgagctct gccacaagaa cagcaaccac agcgccagca gcagatacca aaacagaaac
     5281 tgccagcgtt gccaccggct gcgcggacgc gggcgatgcg gttcaggcgg cgcttcatac
     5341 ggcggcatca gtcgccctcc cccggttccg tccgggtgcg cggtttttcc aaatcggcga
     5401 tgatgtgacc acgccgaaat accgtcacgt gcccagtgga ttgactgacg gcaatcgcga
     5461 cggcgttcgt gacggccgta atcccggcgg ccgcctggtg ccgcgctccc aagccggccg
     5521 gcaggtcatg aatgtcgcgc gtcgacgcgg tcaggtaggt gcccgccgcc tcgatcacgc
     5581 cgtcgccgcg gattatgaac gcgccatcga tcgaagacag ttccttcacg gtctccttca
     5641 gctcgggcgc gagaatattg cgggcttcct caggatagcc cttaaatgga ttcaagatta
     5701 gctgccgcga cagtgccaac acgttctggg tgtccccgac cacaaacagt ccgcccacca
     5761 ttttgccttc gcggccttcg tagcccagtt cggccgcgat gttcagcaca cgttccagca
     5821 cgtccgggcg cacgtcgcgc gacaggtccg attcagttgc ggcgtccagc ggcatgtcta
     5881 actcggagcg cacgtgcagc accagcagcg tgtccagcga gctgccgccc ggcatgccgg
     5941 tcaacacgac taccacctcg tccggttcga tcagtttgcg cgacaccgcc agcagaatcg
     6001 cgacctgggc ctgccccatg cggctgagcc gcacgttcgg cacgcgcaga atgcgatggc
     6061 cccgctcctc gtgctgttct tcctggcggc gagttttcgt gacgtaaagc agccgcgacg
     6121 caatctcggg cggcagctcg agctcttgcg catcaagcgc ttcggcgtac accagggcag
     6181 ccgccgcgcc gcattcttga ataagctgcg gcagcgttct gatgagcgcg tcccggatgc
     6241 ggcgctcgat gctgaccagc gtgaccggcg ccgcaagctc gtcgcgccgc acgatttggc
     6301 ttgcgtcacc agccatgaac aacactcgaa gactcgacca ggtttcgggg cgaatacatg
     6361 acgttctcga taccggccat ttcccgacgc tacgatgtta tgcgccaaac gttctgcatc
     6421 cggctgcttg tcttattgcc ggccgctggc ataatcgcgc cccggcgttg gcggaccatc
     6481 gacgacgttt caggcaaagg taaagcatga gcaatgtagg cttactcggc gggctgccca
     6541 ccgagttccc ccgcgcatac gtggccaagg aaacggaatt cgctgactgg cccacggccg
     6601 aaagctacta ccgcgagtta ctgggccgta acgtcggctc ggccgcgcag ctcgagcgtt
     6661 ggctgtacga tgccagcgaa ctggaggctt gtctggacga ggagcgttgc gcgcgctacg
     6721 tcgaggccac ccggcaaacc gatgatgaat cgcgccagca gcgttacatc cagttcgtcg
     6781 agcaggtcga agcacgccag aagccctggc aggacaagct gctccgccgc ctgctggatc
     6841 tggccgatca atttccgctg ccgccgctgc gctacgaggt cctgctgcgc cgcgctcgca
     6901 atcgcaccga gctgtatcgg gaagagaaca tcccgctgct gaccgaggag gcaaagctgg
     6961 tcagcgaata ccaaaagatc accggcgcca tgacttgccg ctacgacggc cgcgagcaga
     7021 cgctgcagca gatggcccgc taccaggagg aaaccgaccg cagcgtgcgc gaggaagcct
     7081 ggcgcctcat ggcagaccgc ttccagcagg atgccgagcg cctggacgaa ctcttcgtca
     7141 aaatgacggt cttgcgcgac cgcatcgccc ggaacgccgg ctgtgccgat taccgcgact
     7201 acagtttcaa gaactacggc cgcttcgact acacacccgc ggattgcctg gcttttcatg
     7261 acgctatcga gcgctgcgtc gtacccgcgg cccgcgagtt ggccgagcaa cgccgccgca
     7321 aactgggcct gaaagccctg cggccgtggg acttcaacgt cgatccggct ggccgcccgc
     7381 cgctgcgacc gtttgagaat gtggaccagc ttgtgcaagg ttgcagccgg attttccaac
     7441 gcgtgaaccc ggagctgggc cgcatattcg acaaactggc agccctgggc gtcctggacc
     7501 tcgacagccg caagggcaaa gcgcccggcg gctaccagga gaccttcgcc gagcaccggc
     7561 tgccgttcat cttcatgaat gcggttggca ccgacaacga cgttcgcacg ctgctccatg
     7621 agggcgggca cgccttccac agttgggcct gccgcaacga accgctgctg ccctaccgca
     7681 gtgccgacgg ggcgatcgag ttcgccgagg tggcctcgat gggcatggaa tgcctggcgc
     7741 tgccgcacgt ggaaattttc ttcggcccgg aaacgcaacg ggccaacaaa cgctatttcg
     7801 agcggatcgt gcagttcttc ccgtacatgg cgcgcgtcga cgcgttccaa cactacgtct
     7861 acacgcacct ggacgccggc ctcagcggct ggaaggacca ctggcaggcc ctgacgcgcc
     7921 gcttctcgcc cgaggttgat tggtccggcc tcgaagccta cgacagccgg tcatggcagc
     7981 gcaagctgca catttacgag gtcccgttct actacgtcga gtacggaatc gcgcagctcg
     8041 gggccctgca ggtctggcgc aactccctgc gcgactacgc ccacgccgtg gccctttatc
     8101 agaatggcct ggccctgggc ggctcgcgcc cgctgccgga actcttcgaa gccgccggct
     8161 gccagttcga cttcaccgaa gcgacgctgc ggccgctgat tgaagccgta atggccgaga
     8221 ccggcgccgt ggcaccgggc gccgtgccca agctgtaaac cgggtgctcg gacaccgaat
     8281 tctgcgccgg gtgccatgcc caagctgtaa gcgcgggcat gccctccctt aacgcgcggc
     8341 cgcaccccac attcccactt tcccacattc ccactttcct acattgccac ttttcccaca
     8401 gtcacacgtg gcaaaatggg gccaattgcc gaacgcatgc ccgcgcccgc aacgcgggct
     8461 tgggcatggc acccacgggc gccgggaatt ccgcgcgggt acgcgccggg cggccttgct
     8521 cgtggtacat tgtttaatcg atcccggtgc cggctcattt ttcgtcccgg tgccggggtt
     8581 ttattcgtcc tggcgccggg cttggtaccg gccttgccag ggctggcggc gacgtccgga
     8641 ggacgaggag tacgtgacgc tcgaaacggc gacagaaaaa gaaaagagcg gcttcgtgcc
     8701 ccagcggcca cacgagttga gtgaagtgca cccgcgccaa gcacgccggc cctggctgcg
     8761 aaagctgatc cccgcggcgc tcatcgtcct tgccctggcc ggtgtgtact gcatcgccat
     8821 gctccccaag gcggctgatg acgctccccc aatcgagatt ccaccagtaa acgtgtcggt
     8881 aatgaccgtt accgccgtgc ccgaaatgcc ggactcagtc acgctcagcg ccgtcgtcga
     8941 gcccgactac gtcgtccgcg tcgcggcgga agtcgccggc cgcatcgaaa aatggggcca
     9001 gcggcgctcg gacgcctact ggctggggcg ccactatccg gccggcacga ccgttctgga
     9061 gggcgagccc gtcaccgagg gcgacgaaat cctgtacctc aatacggatc tgctcaaagc
     9121 gcgtttcgac cgggctgtgg ctcaatatga gttcgacgag cgcgagtggc gtcgcatctt
     9181 gaacctgtcc gaaaccgggg cgaccacaac caacgaactg gccgacgcac gcaccaagcg
     9241 cgacatgagc aaggccgccc tggacgaggt cacgcgtcaa cttgagcggg ccgtcatcgt
     9301 cgccccgatc agcggcgtcc tgaataagca gtgcatgaag atcggcgaat acgcgagccc
     9361 cggcgaacgc gtggccgaaa tcgtcaagct cgacatcatg aaagtcgtcg tggacgtgcc
     9421 cgaacgcgac gcgccctacc tgaagatcgg cgacctcgcc accatcttca ccggcccgcc
     9481 gcgtgaggcc gagctaaccg ggaaaatatc gtatatcagc gaactggctc atgcgggcag
     9541 ccgcaccacg cgcgtcgaaa tcgccgtcga gaaccgcaac cagttgctgc gcagcgggca
     9601 aatcgtgcga acgcggctga cgcggcgcat gctgcaaaat gtgatcatga tcccgctggg
     9661 agccgtcata ccgctcgaag acggcaaact ggtttacata gtgaaggacg gcaaggccga
     9721 acggcgcgac gtgcagctcg gcttcatccg cgctcgcgag gtgcaagccg tcagcggcct
     9781 cgagcccggc gacctcctga tagttgaagg tcaccgctac gtcggccccg ggcaggccgt
     9841 acgagtcgtg ggagagtact agcatgccac gcgccagcgt cagcgccccc gcacttccca
     9901 cattcccaca ttcccgcatt cccactttcg cccgggtgcc atgcccaagc tctaagcgcg
     9961 ggcatgccag gacggcccct ccccacattc ccacattccc actttcccac tttcccacat
    10021 tcacacgacg gaaggccggg ccgcagccgt gatcctctcc gacctggccg tcaagaaccg
    10081 cactactgtc tgggtctttt ccctgctggt agtggtcttc ggactgtaca gctactttac
    10141 cctgccgcgc gaagcttctc ccgacgtgcc gatcccgatc attctcgtct caacaatcta
    10201 cgagggcgtc gccccgcagg acatcgagtc ggccgtcacc atcaagctcg aacgccagtt
    10261 ggccggcctc aaaggcgtca aggagatttc ctcccacagc gccgagggca tctcgatcat
    10321 cgtcatcgag ttcctgccca acgtgaagat cgatgacgcc atgcagtacg tccgcgacaa
    10381 gatcgatctg gctaagaaag acctccctca agaggccgag gaaaccacac tcacggaaat
    10441 caacgtcgcc gagttcccca tcctgatggt caacatctct ggggatgact cgccctggcg
    10501 gcagcggctg ctcgccgacc gcctggagga cgagatcgag gccatcccgg gcgtgctgaa
    10561 ctgcgacgtg ctgggcgcgc tggagcgcga aatccgcttg gagatcgacc aggacaaggt
    10621 tgccgcctac ggcctgacca tcccggaaat cctcaagctc atcccgtcgg agaacgtgaa
    10681 catctccgcc ggcggcctcg atacccccgg cacgaaattc aacgtccgcg tgccggccga
    10741 attcgtcgaa ccggaagaag tggatgagct cattatcgct gagcgcgacg gccacccgat
    10801 ttacctcacc gacgtcgccg cggtgcacga cacattcaag gaccgggtcg ccttctcgcg
    10861 catgaacggc aaggagagca tcaccgtctc cgtccaaaag cggttgggcg agaacatcat
    10921 cgacatcacg gacgccgtga agcgggtgct ggccgaattc cagcgccgcg ccccggccgg
    10981 catgcgcttc gagatcaccc tcgatcagtc caaggacatt cgctcgatgg tggccgatct
    11041 ggagaacaac ctgatctccg gcatgatcct ggtagtggcc gtcatggtga tcttcatggg
    11101 ctggcggccg tcgctgatcg tggccctcga aatcccgatg agcatgctgc tgacctatac
    11161 cacgctgcag ttgctgggct acacgctgaa catgatcgtg ctgttcagcc tgatcatggc
    11221 cgtcggcatg gtggtcgaca acgccatcgt catggtcgag aacatctacc gccactacca
    11281 gctcagcggc cgccgcatcc aagccgccat gacgggcgcc ggcgaggtcg cctggccggt
    11341 catcacctcg acgctgacga ccgtggcggc cttcgctccg ctgatcttct ggccgggcat
    11401 catgggcggg ttcatgaagt acctgcccat cacgctgatc atcgcgctcc tggcgtcgct
    11461 gctgatcgcg ctggtcgtca atccgatgct cagctcgatc ttcgtcacgc gcgagaaaac
    11521 gcacgggccg agtcgcttta tgcacggcta ccggcgattg ctggcgaccg cgctcgaaca
    11581 tcgctttacc acgctttgcc ttgcagtcct gctgctggtc ggcctggtaa tcctgtacgg
    11641 caaacagggg catggcgtcg agctgttccc ggaattcgat ccgcgccgcg ccatcatcga
    11701 cattcgctgc ccgcagggca cgaacatccg cgagaccgat cgcctggccc gcactatcga
    11761 gcagcgcctg gagccgtttg ccgccgatct cgaacgcgtc atcaccaacg tcgggcacag
    11821 cggcgggggt gaaggcaatc tcggcggctc ggcggccggc gagcacctgg ccagcatcgt
    11881 cctgactttc cacgactacg aaatccgccg ccggccctcg accgaggtta ttgcagagat
    11941 tcgccaagct atcgccgacc tgcccggcgc ggagatcaag gtccagaagc aggaggaagg
    12001 gccgcccacc ggggccgcgg tcacggtccg cctggtcggc gaggacttca aggtgctcga
    12061 aaacctcagc gaacaggccc gcaatctgat tgcaaatgtg cccggtctgg tcaacctgcg
    12121 cagcgaccac gaggccgccc ggcccgaatt ggcctttcgc gtggaccgcc gccgggcgat
    12181 gctgctgggc gtcaacaccg ccacggtcgg caacttcctc aagacggccg tgttcggctc
    12241 ggaggtgggc aagtaccgcc agtacaacga cgagtatgac atcaccgtgc ggctgccgct
    12301 ttcgcagcgg cagagcatcg acgacctgtt ccgcctgcgc gtgccgaacg ccaacggcga
    12361 agcggtgccg ctcagctcgc tgggcgactt cgagtacacc ggcgggtacg gcattatcag
    12421 ccggatcaac cagaagcgcg tgatcacgct tatgggcgac gccgaggggc ggctggatac
    12481 ggcggtgctg gccgacgtgc aggaacgcct gaaagagctc gatctgccgc tgggttatga
    12541 ggtgcgctat gcgggacaga aggaagagca ggacgaagcg caggccttcc tgacgaaggc
    12601 gttcttcatc gcgctgttct tgatcttgat gatccttgtg acgcagttca acacgctgat
    12661 cgtgccgctc atcatcatga ccaccgtgat tctctcgctg atcggcgtgt tcgcgggact
    12721 gctgatttgc aagatgccct tcggcatcat catgaccggc atcggcgtaa tcagcctggc
    12781 cggcgtggtg gtgaacaacg ccatcgtgct gctcgactac acccggcaac tgcaacgccg
    12841 cggcatcgat cttatcgcgg cctcgatcga ggcgggcgcg acccgcttgc ggccggtact
    12901 gctcacggcg tgtacgacga tcctgggcct gatcccaatg gccctgggca tgagctacga
    12961 cttccacaag ttcgaatggg cgtggaagag cgaatcgagc caatggtggg ccagcatggc
    13021 catcgcggtc atcttcggcc tggcgttcgc cacgattctg acgctggttg tggtgccgtg
    13081 cctctacgtc acgctcaccc gcctgacgac gcgcctgggc ctcgggcaag cggcggaaga
    13141 ggctgaaatt cccgacgacg cttgtgaggt gataaggtga tagagtgata gggtgataaa
    13201 ggacggtctg cggacccctt tctcacccta tcactttatc actctatcac cccgccctgt
    13261 ccgccatcat caaaccgagc agcagcggta aatagaggtt gctcgccagg aatacgcgcc
    13321 gcgcgccgct cacgctccgc gcccgccccc agccgacgga caccaacaac agcgctccgc
    13381 ccagcacgac cgacccgacg gcgtatgccg ggccgcaaac gcctgccagc accatcccgt
    13441 atgccgccgg aatcagcaac gcacagcagg ccgcgaccat gaggcaggtc actctgccgc
    13501 tacgatcagc gaccggcagc atccggaaac ccgctcgcgc atagtcttca cgatagaccc
    13561 acgccagcga gaggaagtgc gcgatctgcc atatgaacag caagccgcct agcgtcaatg
    13621 ccccgacttc gatgcgcccc gtcgcccccg tccacccgat tatcggcgga agcgccccca
    13681 ccagcccgcc gaccagcgtg cttagcggcg tcaccggctt cagtggggta tatagcccga
    13741 cgtagatcac caggtttacg gccgccagcc acgcactcag cccgttcacc gtccaccaga
    13801 gcagtgccca tcccgccact ccgctcgcga ggccgaaaac acgggccgct ccaaggctca
    13861 gcgccccgct aggcaaaggc cgccggcaag tgcgtgtcat ccggctgtca cgcgaaacct
    13921 ccgaacactg gttcagcgcg gccgacccgc aggcggtcag agccgtgcct aatagcgtcc
    13981 acagcagccg tttccagtcg atgtcgccgg acgcgagcac gaatccgccc agcgtggtca
    14041 atagcaccat ggcacagacg cgcactttgg ccaattcggc gtagagggaa agcaggtggc
    14101 gagtctggcc ggcgacggcg gcgcggtgag tcacggcgac agtgcgatca cttggctcga
    14161 cggcgcgacc atctggctcg atggcgcgat cggcaaaatc caaggtggcg ggtggcgcgg
    14221 gcaatcgatg cttccttcca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 ntgtggatgg catggcggac aacatgtatt gtgcacggca tggacggacg gatcatcctc
    14401 gaaactgagc gcgtcgtact gcgcgaagtg acgctggccg acctcaactt cgtcgccgac
    14461 atggtcggca atcgcgaagt gatgcgcttc tggccgcggc tgctttcgcg cgatgaagcc
    14521 cggcagggac tgcagaaaca tctggccgac tatcagcgct acgggcacgg ctactggctc
    14581 gccgtgaacc ggcagacggg cgagcccatt ggccaggccg gtctgctgat gagtgaagtc
    14641 gagggcgtgc tgcatcccgc gctgggttac atgctccatc ggccattctg ggggcagggt
    14701 tatgccacgg aatgcggggc cgcggtcctg aagtgggcgt tcgagcagcg ggactaccag
    14761 cgcgtgatat gcctgattcg gccggagaat attccgtcgt tacgtgtcgc gattcgcctg
    14821 gggctgcggc ccgaaagcta cgttgtgttt aagggcttca atcacctggt cttcgtagcg
    14881 cggcgcaccg gtccttgagt tgcggacccg ttttacgccc agtcgccccc tccaaaagca
    14941 gttaaccggc tggtcgcccg caaaaaaggt aaccgcgggc cgagactaca ttcctgaaag
    15001 ggtcgccggt ttggcgcgcg gcgcgttgac gttggcggga actctgtata caccatgcgt
    15061 catggccaca aaagccccaa tacggctggt cgttgttacg cccgagcgcc aggtggtgtc
    15121 ggaacagacc gcttcggtag tgttcgccgc gcacgatggc gagttaggcg tgctgcacaa
    15181 ccgcgcgcca ctcatgtgtg agctgggcat cgggcaactc cggtacgaag ccggcggcca
    15241 gacgcgccgt tgcttcgttg acggcggctt cgcgcaggtg cgcgaggaca cagtaaccat
    15301 tcttgcacat caggcgatcc cagccgaaca ggtcacgccc gagatggttg gtgaggccga
    15361 gcgcaagctg gaagagctgc gtggcaccgg ccccgaggcg caggaagagc ggagcaaggc
    15421 gcaacagcgg gcgagtgcgc tgcgccggat tctcgcaaac gagtaagcag gcaaacgagc
    15481 aggaagacca gagccggggc gtgtcgcccc ggaatcgacc aggtgccccc agcacgcggg
    15541 ccacccagcg cccgccacaa ttaactaacc agccaattgc tgatatccgg cggcaaccgg
    15601 gccggcatcg ccggagccgc gcggttcgag cataccgacc aggcggccgt ctttaagaat
    15661 cagcacgcga tcggcgtaag cggcgcccag ggcttcgtgc gtcaccatga gaattgtctt
    15721 gccctgctcg agcacgattc tgcgcatgag ctgccagatt tcgaccgatt ggcgcgagtc
    15781 aagatttccg gtgggctcgt ccgccagaac gatggcgggg tcgttgagca gagcgcgggc
    15841 gatggccacg cgctgctgct cgccgcccga gagagcgtcg gggcggtggt cggcgcgttc
    15901 ctccaggtgg acggcgcgca gcaggtcgtc gattcgcgtc tgaaactgat cggggttttg
    15961 gccgtccacc agcagtggca gcgcgacatt ctcgcgggcc gaaagggtcg gcaataggtt
    16021 gtactcttga aagattattc ccagccgccg gcgtcggaag agggttcgtt gccgatctga
    16081 aagagccccc aggttcacgt tctcaacgag cacctcgccg gaggttggct gagtcaggcc
    16141 gccgatcagg tgcagcagcg tgctcttgcc ggagccggaa gcgcccatga tggcgatgaa
    16201 gcagccggcc ggcacggtga agctgacgcc attcagagcc gtaaccgggc ggcctgaggt
    16261 ggcgtaaacc ttgaccaggt cgcggacctg gacgatctgc gagtcgacgt ccgcagactc
    16321 ggcgacaggg ctgtccttaa tctcggggtt catccgcaag gcagtttagg cgcgcagcaa
    16381 gcgccgcaca acgcgttcca cgcccctcgc ctctagatcc ctcgatccct agatccctga
    16441 tccctaaatc cctacgcatg aatttcgcgc ccgctcaccg ccagggccgc ttccttcagc
    16501 gcttccgcca gcgtcggatg cgcgtgcgag cagcggccca gatcctcggc gctcgcgccg
    16561 aaggcgatgg ccacggccgc ttcggcaatc aggtcgccgg cacgcggacc gattatgtgt
    16621 acgccaagca cgcgatctgt cttagcgtcg gccaagatct ttacgcggcc gtcaacctga
    16681 ccgagcgcgc gggcccggcc gttgccgcgg aactgaaaga cgcccttttt gtactcggtg
    16741 cccagttgct tgagctgctc ttcggtcttg cccacgccgg ccacttcggg ctcggtgtaa
    16801 cagacggcgg ggatggcatc gtaattgacc tcgccgaagc ctgtgaccag gcggtcgacg
    16861 caggcgatac cttcttcgga ggctttgtgg gccagcatcg ggccacggat gacgtcgcca
    16921 atggcgtaga tgccgggcgc ggtcgtagcg aagttgtcgt ccaccgggat tcgccgcttc
    16981 tcgtccagct tgatccctac ggactccagg cccagccctt cggtgttggg gacccggcca
    17041 accgatagca ggacgcgatc gcactccagc ggcgccgcgc cttcggcctc gacaatgcac
    17101 ttttcgccaa ccgtacgagc gccggtcaca cggctgccca agcggaattc catcccctgc
    17161 cgcttgaaca gcttgagcgc ttcggcggcg atttcggagt cagtgttcgg gagaatgtga
    17221 tcgaggtact ccaggacggt gaccttggcc ccgaggcggg cccagaccgt gccgagctcc
    17281 aggccgatgt atccagcgcc gatgaccacc aggtgcttgg gcacctcggg gtaactaagg
    17341 gcctcagtgc tggtgccgat gcgctggccg tcgggtttga cgcccggcag ttcggccgag
    17401 cggctgccgg tggcaatgat gatgttcttc gcgctgattt cctcgagccc accgcccggc
    17461 gcagggctga ccgacggcac cccaccctcg ccagccaact gcggcggtgc cagcggctca
    17521 accaccacgc ggccgggccc ggcaatgcgg ccgcgacccg cgtagcgcgt aaccttgttc
    17581 ttcttgaaca gggcctcgat gcccttggtc aggccggaga cgatcttttc cttgcggttg
    17641 agcatggccg acaggtcgag ctcgacgccg gcgaccttga cgccgtgctc ggccaacgag
    17701 tgccgggcgg cggcgaagcg ctcagaagat tcaagcaagg ccttgctggg gatgcagcca
    17761 acgcggaggc aggttccgcc gagggccgtt tccatttcga cgcaggcgac gttcagaccg
    17821 agttgagcgg cacggatcgc ggcgacgtag ccgcccggac cggagccgat gacgacgaga
    17881 tcgtgttgct tcacttcagt actccaaaaa gtttttggcc attagagccg gaacttgcgc
    17941 ggcgtgccac tgaagcagcg ggcaggggcg gtccacccgg gccgccactg gacaacaggg
    18001 cccggggaac ctaccgttga gtcatctgag cgaggttcgc cgtggcatac accgtcctgg
    18061 cccgaaaata ccgcagccgc accttcgagg atgtgatcgg tcagcagccg atcgccacca
    18121 cgctcgtcaa tgccatcaag agcgggcgga ttcatcacgg gtacttgttc accggcacgc
    18181 gcggcgtggg caaaaccagc atggcccgca tcctggccaa ggcgctgaac tgcctgaagg
    18241 ccgaggcgcc cacacccacg ccctgcgggg agtgcgacgt ctgccgggcg atcgccgaag
    18301 gccaggacat cgacgtcatc gaaatagacg cggccagcaa caccggcgtg gacaacattc
    18361 gcgagctgcg cagcaacacg gcctatcggc cggcccgcgc ccgctacaag atttacatca
    18421 tcgacgaagt tcacatgctg tcgaccggcg ccttcaacgc gctgctcaag acgctggaag
    18481 agccgccgga gcacgtgaaa ttcatattgg ccacaaccga gattcagaag gtgccggcca
    18541 ccattcagag ccgctgccag cggttcgtgt tccgcaacat cggcccggcg gatatcgaga
    18601 agcggctggc cgaaatcgtg cgggccgagg gggccaaggc tgaggaagcc gtgctgcgaa
    18661 gaattgcgcg gctggccaac ggttcgatgc gggatgcgtt aagcatcctg gatcagcttc
    18721 tgtcagtatc accgacgaag ttgacggccg aattgctcga cgaaatcctg ccgcccgcgc
    18781 aggacgaaca gattttcgga ttgctgaagg atgccgccgc gggcgatgcg gcagcggtct
    18841 tggcggatgt tgacaagatt ctggccggcg ggcgcgggct ggacgtgttc tgcaacgaca
    18901 ccatcgaggc cgtacgggca ttgatgctgg tccgggtgtg cggcgtgaac gcgccgatgg
    18961 tcgatttgcc ggccggaact caagaagagt acgtgcggct gagcggacaa ttcgagctgt
    19021 cgcagtacgt gcagatgatt tcgatgctgg aggagttgcg ccgaaacgtg cgtttcagcg
    19081 gggcggggcg ggccctgacg gacgcgtgca tggtgcggct ggcgcggatg cggcagtggg
    19141 ccccgatcga gcggctgctg gccgagttgc cggaaagcgc ggacggtagc gaaaaaaaaa
    19201 agccgccagt cagcgagcgg gcggtagccg ctgactctcg cgcgcagacg gccaactcct
    19261 ccgctcccgg cacctcggcg tatggcgctg gcgtgggccc ggtgtcgcaa cagcgg
//
LOCUS       DAOSYS010000039        19232 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2346_length_19232_cov_4.603692, whole genome shotgun sequence.
ACCESSION   DAOSYS010000039 DAOSYS010000000
VERSION     DAOSYS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19232
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2346_length_19232_cov_4.603692"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLU99_03955"
     CDS             <1..387
                     /locus_tag="PLU99_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOO16242.1"
                     /translation="CMKYAASAEDRASLAAQRGYVLMMHARSRACAALRAERYDDALR
                     LVTEGVAALRAASQETLDEQVSFASEMRVLAALREEIMSRMPPDSPARLRHELQEALE
                     GEDYERAALLRDRLTISQKEHKASFP"
     gene            490..1263
                     /locus_tag="PLU99_03960"
     CDS             490..1263
                     /locus_tag="PLU99_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOO16243.1"
                     /translation="MGLMDGKRGFVFGVANEASLAWQIALRAHTEGAQLAFNHFPGPK
                     MERRVRQLAEPIGARLIVPCDVNDDAQIAAAFHQAGETLGPLDFLIHSVAFASHAALS
                     NPFFQTKRADFLQAMEISVYSLVALCRHGLPYLAPGASILTLTYLGATRMIPGYNVMG
                     VCKAALESSMRYLAGELGREKQIRVNALSAGPVRTLSSSAVSGFAKVLRHYPTKAPLA
                     RNVEAEEVGKAGLYLLSDLSSGVTGEVHFVDAGYNVVGW"
     gene            complement(3033..4820)
                     /locus_tag="PLU99_03965"
     CDS             complement(3033..4820)
                     /locus_tag="PLU99_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOO16244.1"
                     /translation="MPNFFTDNADIRFLFDHLDLATLARIQEDDFADARRSPSNGDPG
                     PFDYAPADAADAIDNYRRILEIAGQIAGEVIAPRAEQIDEEGNTLNEDGTVTLHPLVA
                     DNIRRLAQADLMGFTLPRRFGGLNCPNLIYTMAIEIVSRADASLMNIFGLQGIAETIN
                     AFASEDIKGEYLPRFARGEVTGAMVLTEPDAGSDLQAVRLKAFQDDDGKWYLRGVKRF
                     ITNGCGNILLTLARSEPEISDGRGLSLFLSERCDRIRVRHLEHKLGIHGSPTCELVYD
                     DAPARLIGERQRGLITYVMALMNGARVGIAAQSLGIAEAAFRLARTYASTRKQFGGPI
                     ERLPAVAEMVTDMKVAIEAARALVYETSRVCDLENNNLRVLETGLVTDKDEQKRRKEQ
                     GRTLKRLNGMLTPMSKYYASEMCVRVADTALQVLGGSGYMKDYPAERYLRDARITTIY
                     EGTSQLQIVAAVRGVCSGAFERRVTELEETVCTDPLLSELKAKLVAGKESVLAAIAAA
                     KARGTDFMDLMGRKIVDSAAAVLIGHFLLDQANAGNAPAHERKKRVARRFIESELPKV
                     RMNCELVASGDTSAVAEYELLAGPVPAKD"
     gene            complement(5067..6362)
                     /locus_tag="PLU99_03970"
     CDS             complement(5067..6362)
                     /locus_tag="PLU99_03970"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOO16245.1"
                     /translation="MTKLLAKLVYKAFLPVGAVFAFQRRRKRDPAGFDWREYLLGRVP
                     PRPVSANCVWIHGVSLGEINATRTIVEELRRRTPDTVVVISSTTETGLARARELYPRL
                     VVFQFPLDFTFAVREVLKRVRPSVIALMELELWPNLIDEATAYGVPILVVNGRVTERS
                     VQRFQYPLAHRLSRHMCSLLTWVGAQDATYASRFIELGVRPDRVEITGSVKYDAADTS
                     DHIAGQEELADAMGIKTDEPLWVCGSTGPGEEEAILEAYQRLLKDHANLQLAIIPRKP
                     ERFDEVAQLIVRHGYACLRRSSGAPVMPEGVDEPRPVFLGDTMGELRKFYSLATVVFV
                     GRTLVPMGGSDVMEVAGLAKPIIVGPHTENFAEPVNMLLAEGALRQISAPGALATVVS
                     DLLRHPERREQMGQAGREAILSRAGASRKVVDRILEYLV"
     gene            6459..7310
                     /locus_tag="PLU99_03975"
     CDS             6459..7310
                     /locus_tag="PLU99_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16246.1"
                     /translation="MRLRLTPSKAAGIITILGTFVLCQAQNPAAPGRSKPLPPPVAPS
                     GPLDPRVDQILTRLENRQIDDLHAKVSWKLRYVLDLEEDAITKNGEIWYQQQKPVAKF
                     LVSFNNRVSGGRLHKLDERHMFDGRWYIEENSETKTFDKREVRRADDPGDPYRLGEGP
                     FPVPFGQKKADILRQFEVTLGQPGANDPPNTDRLHLVPRPGSDSYAKYAVVDVWVARE
                     GPESGLPVKVEATKKRGTGEVDSFITIEFKQAELNRGFSGSIFKLEPKPGYEVYEERL
                     EPAAPPK"
     gene            7383..8330
                     /locus_tag="PLU99_03980"
     CDS             7383..8330
                     /locus_tag="PLU99_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO16247.1"
                     /translation="MPSILGLDIGGTKTAVVLGDMNGAIYARQAFATRPERGFFATFA
                     ELCTHADEVRRQAERLGRPAEVVSVSIGGPLEIEPGIIHSPPNLPGWDDIPLKNLLKE
                     KFGLPVYIEHDGNAGALAEWYFGAARGARHVIFLTMGTGFGGGLIINGQLYRGACDLA
                     GEVGHIRIADTGPFAFGKAGCWEGFCGGAGISKLAAARFPKRWATSAVTAQDIAALAA
                     GGDADALAVLDEVARRLGYGLAILVDVLNPEVIVIGSLAVRLGETILGAAREEMRREA
                     LPRAAEACRLVPAALGERLGDVASLCAAIQAFGGAAPSP"
     gene            8362..9024
                     /locus_tag="PLU99_03985"
     CDS             8362..9024
                     /locus_tag="PLU99_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOO16248.1"
                     /translation="MRRRNLRILITAGPTREYIDPVRYISNDSSGRMGFALATAAAER
                     GMRVTLIHGPVNLEKPAGVRAVPVVSAAQMLAECQRRWPEHDVLIMAAAVADYTPSRP
                     AAFKRKKSRADLVLKLKATVDILRELAARRGPKQVVIGFALEDRAGRRNAERKLRQKR
                     LDAIVLNRPEAIGARRAAVDVLVAGQGWRELGPQAKNRHARKILRIAEEIALARQNAG
                     PS"
     gene            complement(9106..9996)
                     /locus_tag="PLU99_03990"
     CDS             complement(9106..9996)
                     /locus_tag="PLU99_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO16249.1"
                     /translation="MNSTTPNFFGSHARQWRGFHFVYPVISRRAHGLSIGINLNPDKA
                     CNFDCVYCQVDRSENVSAPGSQLPVLRPEARKVDLAVLERELRTLAGSFGELFSDREF
                     MSVPSEYRRLNDIAFSGDGEPTASPVFSEAARLAAAVRADYKLNEAKIVVITDACFLN
                     RPKVAEALAFLDQHNGEIWAKLDAGTEEAFRRVNRPRGATLEHLLQNILGAARIRPMV
                     IQSLFLRLRGEAPTEEEIAAYVDRLRWIGEGGGRISLVQLYTVARRPAETYAEPLTLD
                     ELEQIAARVRAIGLRAECYP"
     gene            complement(10323..14033)
                     /locus_tag="PLU99_03995"
     CDS             complement(10323..14033)
                     /locus_tag="PLU99_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO16250.1"
                     /translation="MTKFATVLMIDADEREQRAFDQMVRERKLQWEVKVAETMSAARA
                     LLAGSRIDVIIADHQLSDGQSLDLLAEFPGVPFVLITSTLEERSALRTLQRGADDYIV
                     RDSQRQCLELLPLVVEKTLLRRARAAGLALDITERLEAGDASRQSEKKLQSLVESIPD
                     HAVITLDARGRITTWNPGAERIFGWTKDEIIGKPVDVLFTPEDRGAGVPGRELEQARR
                     RGQSRADRWQVRKDGTRFYAAESVGALAGEDGKPPSYVKIASDATQQKLAERVLWEGD
                     KAKGASWAVRSKLAHYAAAAVLGVLALGALWLVSPLFDGKGAPWMLVSLAVAAAAWWG
                     GVGPGLLTTALTSLVGWWAFVPEHSSFAGSDAAEVFRWSAFVASSVAISALAGGIHRA
                     NERQKLSVARLAAEADERRRMEAAVRERAQMLEQSYDAAMAWPLHGTITYWNSGAARL
                     YGFSRAEAIGRSPQELLRTQVDGGLEACFAALERDGRWSGELTQQTRDGRQVIVESRM
                     VLMPDESGRAGVVIESTRDVTERRQAEAKLRESELFYRQTLESIPGMVFTTRPDGYCD
                     YHSQQWEDYTGVPTSQQVGTNWNKLLHPDDQAGAYAAWRAAVEERGSYDVEYRVRRRD
                     GQYEWFNVKGRPIRDSSGQIVRWFGVATNINDLKRSAEALHRGAQNLQLLSKTATRLL
                     MEDSPKEALRDIFPELSAHLGMELFFKFLVSEDEERLILQASGGIDDATVRRLQQADI
                     TESISGVVARQRRAVVVEDVQNSTDPAAALIRSLGLTAYACHPLLARGRLVGTLGFGT
                     RSRIHFNPDELDLLRTVSDQVAMALERKRLVTELEQRADELTVAKASADAATSAAEQA
                     NRAKDRFLAVLSHELRNPLTPVLAAATLLGQGPGVSADMREGLEVIRRNAELEARLID
                     DLLDVTRIARGKLQLNRKPVRVCTILSRAIEVCQPDIDARRLHFGSEIVGGPHVVNVD
                     AARLQQVFWNVLKNAIKFTPNEGCIGARCWREDSLIIVEIRDSGIGIEQEALQRIFNA
                     FEQAEPIMTRRFGGLGLGLAISKAIVEMHGGTIVASSQGKGHGATFTIKLPVHAAQPQ
                     TEPAQPGKAAPALEAAGLRILLVEDHGDTSRIMARLLKSSGHTVQTAGDVATAVLQAQ
                     AKTFDLLISDLGLPDGSGIDLLNQLRASGHDYPAIAMTGFGQEEDIARTRAAGFTAHL
                     VKPIDLEQMTRTIAEAAGPAQQPQPVTPRHGT"
     gene            14677..18354
                     /locus_tag="PLU99_04000"
     CDS             14677..18354
                     /locus_tag="PLU99_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO16251.1"
                     /translation="MKANPPSPLPDAHRVLRESEEPYRSLFEHMLDGCAYCQMLFDEQ
                     GGPDDFVYLAVNEAFGRLTGLKNVVGKRATEVIPRIKELNPELFEIYGRVASTNKPER
                     FEIDFKPLGLYLSVSAYCPEPGHFVAVFDDISERKQAELRLRQAKEEWERTFDSVPDP
                     IAVLDRAHRVRRVNRAMADRLGVTPEQCVGLHCHEAVHGLGAPPAFCPHMLTCADGQT
                     HTVEVHEERLGGHFLVTTTPLRDEQGRVLGSVHVARDITARKRAEEALRASHERLKKV
                     LEVETVGVMFWDLKTGYLTDANDTFLKMTGYSRGDLEAHLLSWQKFAPPEYQEVGLAG
                     IRKFQATGRAGPYEKECVRKDGTRQWLLFAGSSLGDDTCVEFCVDISAQKNAEAALQK
                     AHDELEKRIAERTAQLNQTTELARAERQRFYHVLETLPAYVCLMTPDYRIAFANRIFR
                     EWFGHSLNRKCYEFLFDHDQPCERCQTYSVLKTNAPHRWEWTGPNGRTYDVFDFPFID
                     TDGARLILEMGIDITERKQAEKALQEVNETLEQRVAQRTAELRQSREDLDRAQAVGQI
                     GWWRLDTRKNVLTWSPESHRIFGVPEGTPMSYESFLSTIHPDDRQYVDARWQAALRGE
                     PYDLEHRIVANGHVKWVREKAYLEFDSDRKLLGGFGITQDITARKTAEEALRRSEEFL
                     RRVIDTVPSMIFIKDWEGRFVLVNEALARTYGTTTAEIIGKTDADFNANTEEVEHFRR
                     DDREVMSTRCTKIIPEEPVTHPDGEVRWYSTVKVPLVKPDGTSAWVLGVASDITARKQ
                     AEEELKAAKVSAEQARAIAESANKAKDQFIAVLSHELRTPLTPAVAALSLLRTDSRLP
                     SDVHEDLEMISRNLNLEVRLIADLLDVSRIISGKLHLDKRPADVAVAIREAAKIVSGD
                     LDAKGQTLAIETPGAPYLTLADGARLQQVFWNLLRNSIKFSPERSCITVRARLTPVEG
                     CPMAAQPCPVGRGDCPAAPSTAGDQEPRPANLVVEVIDQGSGIHPDMLPRLFNPFEQD
                     HKARSFGGLGLGLSICRAVVEMHGGTISAHSEGVGCGATFTVRLPIAQCQRPSPSEQP
                     VRNPSGEAAETRPRDRALRILLVEDHADTAKLMRRLLMAEGYEVVTAGSVAEALAAAE
                     RHRPDVLISDLGLPDGSGLNLMAQLIARGQHIPGIALSGYGTPADLEQSRATGFAEHL
                     VKPLHSMDVVTAAIARLNLNA"
     gene            18641..19186
                     /locus_tag="PLU99_04005"
     CDS             18641..19186
                     /locus_tag="PLU99_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16252.1"
                     /translation="MPGRVSTGEPPRVPAVASASVPTAQPLSPQDSCLRVPPVRLSAA
                     HSPRSAVLTPAAVLLLLVLGLTCMKRALIHPLRNYLLDFRSYYASGLALRAGLNPYDT
                     AAVKSFVQLPGRQSAPPYVYSPPTLALCALASYLPYPSAQTPWLLLQFALAGAAFVLT
                     LRWLRRPWYSQAAALTGWCSS"
BASE COUNT         3520 a         6266 c         6055 g         3391 t
ORIGIN      
        1 tgcatgaagt atgccgcgtc ggccgaggac cgcgccagcc tggccgcgca acgtggctac
       61 gtattgatga tgcatgcgcg ttcgcgagcc tgcgctgccc tgcgggcgga gcgctacgac
      121 gacgccctgc gtctcgtgac ggaaggcgtg gccgcgctgc gggccgcgag ccaggagacg
      181 ctggacgagc aagtctcgtt cgcgtccgaa atgcgcgtcc tggcggcgct gcgcgaggaa
      241 atcatgtcca ggatgccgcc cgactcgccc gcccgccttc gccacgagct gcaagaggcg
      301 ctggagggcg aggattacga gcgtgctgct ttgctgcggg accgcctaac catctcccaa
      361 aaggaacaca aagcttcctt cccttgaatt ctcctgtcct ccgcgcccgc gggccttgcc
      421 cgaaacatcc catagatgct ttattggtgc gctccggaaa atcgcccacc acagctatac
      481 aattctgcaa tgggtttaat ggacgggaaa agaggttttg tcttcggcgt ggccaacgag
      541 gcttcgctcg cgtggcagat tgcgctgcgc gcccacactg aaggcgcgca attggcgttc
      601 aaccatttcc ccggtccgaa gatggaacgc cgcgttcgcc agttggccga acccatcgga
      661 gcgcggctga tcgtgccgtg cgacgtgaat gacgacgcac agatcgcggc tgctttccac
      721 caagcgggcg aaacgctggg cccgctggat tttctgatcc attcggtcgc ctttgccagc
      781 cacgcagccc tcagcaaccc attcttccag acaaaacggg ccgactttct ccaggcgatg
      841 gagattagcg tctactcgct ggtagcactg tgccggcacg ggctgccata ccttgcgccg
      901 ggcgcatcga ttctgacact cacgtatctc ggggcgacca gaatgatccc cggctacaac
      961 gtcatgggag tctgcaaagc ggccttggaa tcatccatgc gctatctggc cggcgaattg
     1021 ggccgcgaga agcaaattcg agtcaacgcc ctgtcggccg gtccggtgcg gacgctctcc
     1081 agctccgcgg taagcggctt cgccaaagtg ctgcggcact atccgacgaa ggctccgctg
     1141 gcccgcaatg tcgaagcgga agaggtcggt aaggccgggc tgtacttgct gagcgacctc
     1201 tcgagcggcg taaccggcga agtgcatttt gtagacgccg gatacaacgt tgtcgggtgg
     1261 taaagaggcg gattccttgt ctgtatcggg gcttgcatct ggcaggcgct ttaagacgat
     1321 gtaaccggga tactccggca actccagcat ttggtactgg cgatgaatgt gcggcaccga
     1381 cgcgaggtcg cggatgtgca tccagagcca atacgtgacc aggtaacgtg gggcaaactc
     1441 gtcgactacc gccgcgaccc attctaccga accctcccga gaggccgcgc cgacggcgct
     1501 cagtcgctcg aagtcggccc tgcgtgcccg gagcactgct ggagagttga tcccggaact
     1561 gtcaagaatc acttgctcgg gtaaggcgta cgccagcgct cccacttccc ccacaaagag
     1621 cacatcgccc gccgcgcagt gcggacgcag caaatcggcg attgcgaggt aagccgccgt
     1681 cctttggccg aagtggtcgt cgtacatcga gcggcgccag gtgtcgcggg cggcgtaacc
     1741 cgtgccggcg agaaccagca gcaccgccgc ggtaactgaa cctgcacgct ggctcgagaa
     1801 cgccgcgatc ggcgccggcc ccgagcgtgg cgaatcgttt gcaggccgtt ctgcggcgcg
     1861 tgccagtctc cccaccacga aagaggcgcc gctgaacatc gcgtaccccg cgaacaaaat
     1921 gatgcccgga aacagcggcg agagatacca gggaaacggt atcggcgtga tgcacgcagc
     1981 cgacaagccg gccaaataga tcagcatcca aaccccgacc agcagcagtc ccgacctgcg
     2041 cacgccggac acgatcagcc ccagcagcgc caatggggac agaaccatca tttcgaatcc
     2101 ggcagtaagg cgctcgacat tcaggcgcaa tatttccagc ggtaattcgg cgtggatgag
     2161 ctgcttggca gcgatcgaat gcggcgctat tgcaccgtac gtgtggtatg cgtacgccag
     2221 ccacggaacg ctcactaccg ctgcgcccaa aagcgctcgc cagcacagcc gcggcgcccg
     2281 ccagaaagtg aaaccgaaca acagggcgat cacaatggcc ccgtcgggac gcaatagaaa
     2341 cgcgattcca gccgccaatc ccgcccagaa gtaccacccg cgcagcgtca acagcagagc
     2401 cccgtagacc gcggccatac tcagcccggt ttccatgccg gaatttgctg taaacgccag
     2461 gtcggggtgc agagccaggc ataagccgat cgcggccgcg ccgatcgagc ccaacggctt
     2521 aagtgcgagc cgctggatga tgagcacgac gcccattatc cccagtaggc ccaacacgcg
     2581 ggccgcgctg ggtatgtccg cccccaacag cctcaacacg ccgagcaggc cggccagcag
     2641 gggggtggtg gtgcccagca ccgcctctcc agggttgaac accaggccgt gaccggccgc
     2701 aagattggcc gcatagcgga acgtgatgta ggcatcgtcc acgacatacg ggccgaacgc
     2761 gagcgcgctt gccagagcca ccagtggcgg gacggcggtc agcaccagcc gcgcccacca
     2821 ggcgcgcggg aaatcgagcg ccccggggac tggttcgctc agcgtgtcac tcgtggaagg
     2881 gtggttcggt cgagccatcc ggcactccgc tcagccggcg gcattctaag tcagtatgga
     2941 tgattcggca gcgcgccagg gcacacgggc catcgtcgtg gcccgagttt ggggctcgaa
     3001 tcgccgagta gggctggtca aacggcgccg cgctagtctt tcgccgggac cggacccgcg
     3061 aggagttcgt attcagccac ggccgacgta tcgccgctgg cgaccagctc gcagttcatg
     3121 cggactttcg gcagctcgct ttcgataaac cggcgtgcca cgcgcttctt gcgctcgtga
     3181 gccggcgcat tcccggcgtt ggcctggtca agcaggaaat ggccgatgag cactgcggcc
     3241 gcgctgtcga cgatcttgcg gcccatcagg tccatgaagt ccgtgccgcg cgcctttgcg
     3301 gccgcgatcg ctgccaggac gctctccttg ccggctacaa gcttggcttt caactcgctg
     3361 agaagcgggt cggtgcagac ggtttcttca agctccgtca cacgccgctc gaaggcgccg
     3421 ctgcacacac cgcgcaccgc agccacgatc tgaagctggc tggtgccctc gtagatggtc
     3481 gtgatgcggg cgtcgcgcag gtaacgctcg gccgggtagt ccttcatgta gcccgagccg
     3541 ccgaggactt gcagagcggt gtcggccacg cgcacgcaca tctcagaggc gtagtatttg
     3601 ctcatcgggg tgagcatgcc gttgagacgc ttcagcgtgc ggccctgctc cttgcggcgt
     3661 ttctgctcgt ccttgtcggt caccaggccg gtttcgagca cgcgcaggtt gttattctca
     3721 aggtcgcaca cgcggctggt ctcgtacacc agggcacggg ccgcctcgat cgcgaccttc
     3781 atatcggtaa ccatctccgc caccgccggc aggcgctcaa tcgggccgcc gaactgcttg
     3841 cgtgtgctgg cgtacgttcg ggccaggcgg aaagcggctt cggcgatgcc cagcgactgg
     3901 gcggcgatgc cgacgcgcgc cccgttcatc agcgccatga cgtacgtgat cagaccgcgc
     3961 tgccgttcgc cgatgagccg ggcaggagcg tcgtcgtaaa ccagctcgca ggtcggggag
     4021 ccgtgaatgc cgagcttgtg ctccaggtgc cgaacgcgga tgcgatcgca gcgctcggaa
     4081 aggaagaggc ttagcccacg gccgtctgat atctcaggtt cgctgcgcgc cagcgtaagc
     4141 agaatgttgc cgcagccatt cgtgatgaac cgcttcacgc cgcgcaagta ccatttgccg
     4201 tcgtcgtcct ggaaggcttt aagtcgcacg gcttgaagat cgctgccggc gtccggctcg
     4261 gtcaacacca tggccccggt gacctcgccg cgggcgaagc gcggcaggta ttcacccttg
     4321 atgtcctccg aggcgaaggc gttgatcgtc tcggcgatgc cttgcaggcc gaagatattc
     4381 atcagcgagg cgtcggcccg gctgacgatc tcgatggcca tcgtgtagat caggttcggg
     4441 caattcagcc cgccgaagcg gcgcggcagc gtgaagccca tcaggtcggc ctgggccagg
     4501 cggcggatgt tgtcggccac gagcgggtgc agcgtgaccg tgccgtcctc attaagcgtg
     4561 ttaccttcct catcgatctg ctcggcgcgc ggggcgatga cctcgcccgc gatctggccg
     4621 gcgatttcga gaatgcggcg gtagttgtcg atcgcgtcgg ccgcatccgc gggagcgtag
     4681 tcaaacgggc cggggtcgcc gttactcggg ctccggcggg cgtcggcaaa gtcgtcctcc
     4741 tggatgcgcg ccagcgtagc caggtcgagg tggtcgaaga ggaagcgaat gtcggcgttg
     4801 tcggtgaaga agttaggcat ataagctctc aattccactc atgcccttca cggggtccgc
     4861 ttggcgctgg gattgcctca tgccgtcccg ctggagggcg tcggccgtgt cgatagccgg
     4921 tctgcgatcc gccagccgga tggcactctt tgatggacgt ggcggctcgc tcggcagcat
     4981 tgtcgcgagg gcgtccgcac cgtcaagccg gaaaggggag tgcggccggc tcccgcacca
     5041 cgtctcctcc gccgggagac catcccctac accaagtatt cgagaattcg gtcgaccact
     5101 ttccgcgatg ccccggcgcg cgacaggatc gcctcccgcc cggcctgccc catttgctcg
     5161 cgccgctccg gatgccgcag caggtcgctg accaccgtcg ccaacgcccc aggcgccgat
     5221 atctgccgca gggccccctc ggccagcagc atattcaccg gctccgcgaa gttctcggta
     5281 tgcggtccga caatgatcgg cttcgccagc cccgccacct ccatcacgtc cgaaccgccc
     5341 atcggcacca gggtgcgccc gacgaacacc accgtcgcca gcgagtaaaa cttccgcagc
     5401 tcgcccatgg tgtcgcccag aaataccggc cgaggctcgt ctacgccctc cggcatcacc
     5461 ggcgcgccgc tgctgcgccg caagcaggcg tagccgtgac gcacgatcaa ctgggccact
     5521 tcgtcgaagc gttccggctt gcgcgggatt attgccagtt gcagatttgc gtgatccttc
     5581 agaagccgct ggtacgcttc aagaatcgcc tcttcttcgc ccggcccagt gctgccgcac
     5641 acccacaagg gctcatccgt cttgatgccc atcgcgtcgg ccagttcctc ctggcccgcg
     5701 atgtggtctg acgtgtccgc cgcgtcgtat ttgaccgagc ctgtgatctc aacgcggtcg
     5761 ggccgcacgc cgagctcaat aaaccgcgac gcataagtgg cgtcctgagc gccgacccaa
     5821 gtcagcaatg agcacatgtg ccggctcagg cggtgcgcca gcggatattg aaagcgctgc
     5881 acgctgcgct cggtgacgcg gccgttgacg accagaatcg gcacgccgta agcggtcgcc
     5941 tcgtcgatca ggttaggcca cagctcaagt tccatcaggg ctatcaccga cggccgaacg
     6001 cgcttcagga cctcgcgcac cgcgaacgtg aaatcgaggg ggaactggaa caccaccaac
     6061 cgcggataca actcgcgcgc ccgggccagc ccggtttcgg tcgtgctgct gatgacgacc
     6121 accgtgtccg gcgtgcggcg ccgcaattcc tcaacgatag ttcgggtagc gttgatctcg
     6181 cccagcgaga cgccgtgtat ccagacgcag ttggccgaga ccggacgcgg cggcacgcgg
     6241 ccgagcaggt actcgcgcca atcgaagccg gccgggtcgc gcttgcgccg gcgttgaaaa
     6301 gcgaacaccg cccccaccgg taagaaggct ttatatacca gcttcgccag cagcttcgtc
     6361 attgggctgt tttgtgcggt caaggccggc ggccgccggt ttgctttatt actggcggtt
     6421 cgtagaatcg gccagtgact ttaccgagga gaaacttcat gcgtctgcga ctcacgcctt
     6481 caaaagcggc gggcatcata actattctgg gcacgttcgt cttgtgccag gcccagaatc
     6541 ccgctgcgcc tggccggtcc aagccgctgc cgccgccggt ggctccgagc ggcccgctgg
     6601 acccgcgtgt ggaccagatt ctgacccggt tggaaaaccg tcagatcgac gatctgcacg
     6661 ccaaggtgag ctggaagctg cgttacgtac tcgacttaga agaggacgcc atcaccaaga
     6721 acggcgaaat ctggtatcag cagcagaagc ccgtcgcgaa gttcctggtc agcttcaaca
     6781 acagagtcag cggcggccgg ctgcacaaac tggacgagcg gcacatgttc gacgggcgct
     6841 ggtacattga ggagaactcc gaaaccaaga cgttcgataa gcgcgaagtg cggcgtgcgg
     6901 acgaccctgg agacccgtac cgcctcggcg aggggccttt ccccgtgccg ttcgggcaga
     6961 agaaagcgga cattctgcgc cagttcgagg tcacgctggg ccagccgggc gccaacgacc
     7021 cgccgaacac tgaccgcttg cacctcgtgc cgcggccggg ctcggattcc tacgccaagt
     7081 acgccgtggt cgacgtgtgg gtagcgcgtg aggggccgga atcgggcctg ccggtcaagg
     7141 tcgaggccac caagaagcgc ggcacgggcg aagtggattc gttcatcacg atcgaattca
     7201 agcaggcgga actgaaccgc ggcttcagcg gctcaatctt caagctcgaa cccaagccgg
     7261 gatacgaggt gtatgaggag cggttggagc cggcggcgcc accgaagtag tcagttgtct
     7321 ggcccgaggc cctgacagtc gagaactgaa agcggcctac ttagacccga aaactccgag
     7381 ccatgccttc aatccttggc ctcgacatcg gcggaaccaa gacggccgtg gttcttggcg
     7441 acatgaacgg ggccatatac gcgcggcaag cctttgcgac gcgcccggag cgtgggttct
     7501 tcgcgacgtt cgccgagctg tgtacgcacg ccgacgaggt tcgccgccaa gcggagcgat
     7561 tgggccggcc tgccgaagtc gtgtcggtct cgattggcgg tccgctcgag attgagcccg
     7621 gaatcattca ctcgccgcct aatctgccgg gctgggacga cattccgctc aaaaacctgc
     7681 tcaaggaaaa atttgggctg cccgtgtaca ttgagcacga cgggaacgcc ggagccctgg
     7741 ccgaatggta tttcggggcg gcccgcggcg cgcgccacgt gatattcctc actatgggca
     7801 cgggtttcgg cggcggactg atcatcaacg gccagcttta ccgaggcgcg tgcgacctgg
     7861 ccggcgaagt cgggcacatt cgcatcgccg acaccgggcc attcgccttc ggcaaagcgg
     7921 gctgctggga gggcttctgc ggcggggccg gaatttcgaa attggccgcg gcgcgctttc
     7981 ccaaacgctg ggcaacgtcc gctgtcacgg cccaggacat cgccgccctc gccgccggcg
     8041 gcgatgccga cgctctggcc gtgttggacg aagtcgcgcg ccgcctcggc tacggactgg
     8101 caatcctggt ggacgtgctc aacccggagg tgatcgtaat cgggtcgctg gccgtccggc
     8161 ttggcgaaac gatattgggc gcagcgcgcg aggaaatgcg gcgtgaggcg ctgccgcggg
     8221 cggcggaagc atgccgcctg gttcccgccg cattgggcga gcgcttgggc gacgtggcct
     8281 cactctgtgc ggccattcag gctttcggtg gcgcagcgcc aagcccatga atgacgtggt
     8341 agtagcggaa agaaggccga tatgaggcgc cgcaacctgc gaatccttat cacagccgga
     8401 ccgacgcgcg agtacataga tccggtgcgc tacatttcga acgactcgtc cgggcggatg
     8461 ggttttgccc tggcgaccgc ggcggccgag cgggggatgc gtgtgacgct gatacacggg
     8521 ccggtcaatc tggagaagcc ggcaggcgtg cgcgccgtgc cagtagtctc ggcggcgcag
     8581 atgctggctg aatgccagag gcgctggccg gagcacgacg tgctcataat ggcggcggcg
     8641 gtggccgact acactccgag ccggccggcg gcgttcaaac gcaagaagtc gcgcgcggac
     8701 cttgttctga agctgaaggc gaccgtggat attcttcgcg agttagcggc gcggcgcggg
     8761 ccgaaacagg tcgtcattgg cttcgcgctg gaggaccgtg cagggcggcg caacgcggag
     8821 cgcaagctgc gtcaaaaacg cctggacgcg atcgtactca accggcccga ggcgatcggg
     8881 gcgcgccgcg cggcggttga cgtcctggtc gcaggccaag gctggcggga actggggccg
     8941 caagcgaaga accgccacgc gcgcaaaatc ctgcgcatcg cggaagagat tgcgttggcc
     9001 cggcaaaacg cgggaccgtc ataacccacc cgtcgggact tccgagcact gttccctggg
     9061 actgtaaact ttatcgtcgg gactgtaaga atttgtcgat cggcgtcacg gatagcactc
     9121 ggcacgcagg ccaatggccc ggacccgcgc cgcgatttgt tccagctcat cgagagtgag
     9181 cggctcagcg tacgtctcgg ccggacgccg cgctaccgtg taaagctgaa ccagcgatat
     9241 ccgcccgccg ccttcgccta tccagcgcaa acggtcaacg taggccgcta tctcctcctc
     9301 ggttggcgcc tccccgcgca acctcaggaa caaagactgg atgaccatcg gccgaatgcg
     9361 cgccgccccc aggatgttct gcagcaggtg ctccagcgtc gcgccgcgcg ggcggttcac
     9421 gcgccgaaac gcctcctcgg tccccgcgtc gagcttggcc caaatttcgc cgttgtgttg
     9481 atcgagaaac gccagcgcct cggccacttt cggccgattc aaaaagcagg cgtccgtaat
     9541 aacgacgatc ttggcctcgt ttaacttgta atccgcgcgc acggccgccg ccagcctggc
     9601 cgcctctgaa aacacgggag aggcggtcgg ctcaccgtcg ccggaaaacg cgatgtcatt
     9661 aagccgtctg tattcagacg gcacggacat aaattccctg tcagaaaaca gctcgccaaa
     9721 gctgccggcc agcgtgcgca actcgcgttc gaggaccgcc aaatctacct ttctcgcctc
     9781 gggccgaagc accggtagct gcgagccggg agcggagacg ttttccgagc ggtccacttg
     9841 gcagtacacg caatcgaaat tacaggcctt gtccggattg agattaattc cgatgctcaa
     9901 cccgtgcgcc cgccgcgaga ttacagggta aacaaaatga aagccgcgcc actggcgggc
     9961 atgcgaaccg aagaaattgg gcgtcgtact attcaagaga gtatgagctc cgaatttttc
    10021 aaaagtttac aaaaaagttg gaggcttggg aatacccggc ctttgcactg agttaagtgg
    10081 gtagcaccac acgaacttct ccacagcgtg tggaggagag ttggcgagat gctgttcggc
    10141 tcgcccttct cgtcagcttg gaaacgggcc cgttctcagg tcggagagaa cgggccccat
    10201 ttttttgcgc ctgacaggca gccgggaccc tcgcttcgac cgggggcggt cggttcgagc
    10261 gcggtcaatt aatagcccta ttcatagatg ttagcagggg cgcagcaggg gggcgccaac
    10321 ccctacgtgc cgtgcctcgg tgtgaccggc tgcggctgct gggctggacc ggccgcttcc
    10381 gcgatcgtcc gcgtcatctg ctctaagtca atcggcttca ccaggtgcgc ggtgaagccc
    10441 gccgcccgcg tgcgtgcgat gtcctcttcc tgtccgaagc cggtcatcgc aatggccggg
    10501 tagtcgtgcc cgctggcgcg caactggttg agcagatcga tgccgctgcc gtcgggcaag
    10561 cccaggtcgc tgatgagcag atcaaaggtc ttggcctgag cctgaagaac ggcggtggcg
    10621 acgtcgcccg ccgtttgcac ggtgtgaccg ctgctcttaa gcagccgggc catgatgcgc
    10681 gacgtgtcgc cgtggtcctc caccagcaaa atccgcagtc cggccgcttc caacgcaggt
    10741 gcggctttgc ccggctgcgc cggttccgtt tgaggttgcg cggcgtggac cgggagcttg
    10801 atggtaaacg tcgccccgtg gcccttgccc tggctggaag ccacgatcgt gccgccatgc
    10861 atttcgacaa tagccttgct gatcgccagc cccagcccca gcccgccgaa cctgcgcgtc
    10921 atgatcggct cagcctgttc gaacgcattg aagatgcgct gtagcgcctc ctgctcaata
    10981 ccgatgccgc tatcccggat ctcaacgatg atcaagctgt cctcacgcca gcagcgcgcg
    11041 cctatgcacc cttcgttcgg cgtgaacttg atcgcgttct tgagcacgtt ccagaacact
    11101 tgttgcaacc gtgccgcgtc aacgttgacg acgtgcgggc cgcctacgat ttccgacccg
    11161 aagtgcagcc gccgcgcgtc gatgtccggc tggcacacct cgatcgcgcg gctcaagatt
    11221 gtgcagaccc gtaccggctt acgattcagt tgcagcttgc cgcgcgcaat ccgcgtcacg
    11281 tcgagcagat catctatcag ccgcgcttcc aattctgcgt tgcgccggat cacctcgaga
    11341 ccctcgcgca tatcggcgct cacgccgggc ccctgtccca gcagtgttgc cgcggccagc
    11401 accggcgtaa gcgggttccg cagctcgtgc gaaagcaccg ccaggaaacg gtccttggcc
    11461 cgattagcct gctccgcggc gctcgtggcc gcatcggcac tcgccttcgc cacggtcaat
    11521 tcgtcggccc gctgctccag ttccgtaacc agccgcttgc gttcgagtgc catcgcgacc
    11581 tggtcggaca cggtgcgcaa caaatcaagt tcgtcggggt tgaagtgaat ccggctccgc
    11641 gtgccgaagc ccagcgtacc gacgagccgt ccgcgcgcca gaagcggatg gcaggcataa
    11701 gcggtcaagc cgagcgatcg gatgagggct gccgccggat cggtggagtt ttgcacgtcc
    11761 tcgaccacga cggcccggcg ctgccgagcc acaactccgc tgatcgactc ggtgatgtcg
    11821 gcctgctgca gcctccggac ggtggcatcg tcgatgccgc ccgaggcttg gaggatgagg
    11881 cgctcctcat cttcactcac gaggaactta aagaacaact ccattcccag atgggcggac
    11941 agctccggga aaatgtcgcg cagcgcttcc ttggggctgt cctccattaa cagcctggtg
    12001 gccgtcttcg acagcagttg caggttctgc gccccgcggt gtaacgcttc cgccgagcgc
    12061 ttcaagtcgt ttatgttggt cgccacgccg aaccagcgca cgatctgccc cgagctgtcg
    12121 cgaatggggc gacctttgac gttgaaccat tcgtactgcc cgtcacggcg gcgcacgcgg
    12181 tattccacat cgtacgaccc ccgctcctca accgccgcgc gccaggcggc gtacgccccg
    12241 gcctggtcgt ccgggtgaag cagcttgttc cagttagtgc cgacttgttg gctcgtcgga
    12301 acccccgtgt agtcctccca ctgctggctg tggtagtcgc aatagccgtc cggacgcgtc
    12361 gtaaagacca tgcccggaat ggactccagc gtctggcggt aaaataactc gctttcgcgc
    12421 agcttcgcct cggcctgccg gcgttccgta acatcgcggg tgctttcgat caccacgccc
    12481 gctcggccgc tttcgtccgg catcagcacc atgcgcgact cgacgatcac ttgacgtccg
    12541 tcgcgggtct gctgcgtcag ctcgcccgac caccgcccgt cgcgctccag cgccgcaaag
    12601 cacgcctcca ggcccccgtc cacttgcgtg cgcagcagtt cctgcggact gcggccaatc
    12661 gcctcagccc gcgaaaaacc gtacagccgc gccgcgccgg agttccaata cgtgatggtt
    12721 ccgtgcagcg gccaggccat ggcggcatcg taagattgct ccagcatctg ggcccgctcg
    12781 cgcaccgcgg cctccattct gcggcgttca tctgcttccg ccgccagccg cgccacgctc
    12841 aacttctgac gctcatttgc ccggtggatg ccgccggcca aggcgctgat cgccacgcta
    12901 ctggcaacga aagccgacca gcggaaaacc tcggcggcgt cgctgcccgc gaaggatgag
    12961 tgctccggaa cgaacgccca ccatccgacc aagctggtca gggcagtcgt gagcaacccc
    13021 gggccgactc cgccccacca tgccgccgcc gcgaccgcca ggctgacgag catccatggc
    13081 gcacctttgc catcgaaaag cggactgaca agccagagag cgcccagagc cagcacgccg
    13141 aggaccgccg ctgccgcata gtgggccagt ttcgagcgaa cggcccagga agctcccttc
    13201 gccttgtcac cttcccacag cacacgctca gccagcttct gttgcgtcgc gtccgacgca
    13261 atcttcacgt atgagggcgg cttcccgtcc tcgccggcta gcgcccccac agattcggcc
    13321 gcgtaaaaac gcgtcccgtc ttttcgcacc tgccagcggt cggctcgcga ctgcccgcgg
    13381 cggcgggcct gctccagctc gcgccccggc acgcccgcgc cacggtcctc cggcgtgaac
    13441 agcacgtcca ccggcttccc gatgatctcg tcctttgtcc agccgaaaat gcgctccgcc
    13501 cctgggttcc acgttgtgat ccgccctcgg gcatcaagag tgataacggc gtggtcgggg
    13561 atgctctcca ccagcgattg gagcttcttt tcgctctggc gcgaggcgtc gccggcttct
    13621 aaacgctctg taatgtcgag cgccaggccc gctgctcggg ccctgcggag aagagtcttc
    13681 tccacgacca gcggcaacag ctccaaacat tgtctttggg agtcccttac aatgtagtca
    13741 tccgccccgc gttgtaatgt gcgtaaggcc gaccgctctt caagcgtgct ggtgatcagc
    13801 acaaatggga cgccgggaaa ttcggcgagc aggtctaacg actgcccgtc cgacagttga
    13861 tgatcggcga taatgacatc gatccgtgac ccggccagaa gcgcgcgggc tgcgctcata
    13921 gtctcggcga ccttcacctc ccattgcagt ttcctttcgc gcaccatctg atcgaacgca
    13981 cgctgctcgc gctcgtcggc atcgatcatc agcactgtgg caaatttagt catacccctg
    14041 tctacgttcc cgtcgcgtcg aatcggctgg aagatttgca atcctgttta atatgaggct
    14101 ggctcaatgc acgtcccgat gcacgccggc gcccgcttcg gccgggaagt gcggcaagag
    14161 tgcccggttc gcagtccggc acgcaggaaa cggcggcata acggcctcca ggggcgcgct
    14221 cgctgaggcg aaggtagttt acgttgcgaa atgcacaatt tccacgattt tctgaaagac
    14281 ctgcaacatg gcgcaacgtt gatcccttaa tcccctcgga tcttcttgac cacggcgcca
    14341 tcttaaaaag cagtaatctg ccgcccactc cgcctcgcac tcacgattgg aacgtgccgt
    14401 gacgaagagg cgagcgcggg ccagccccat gaggccagcc aacggcaggg gggctgcttt
    14461 ttgcaccccg agctcaattc gggccgcgtg attatgttga gtttcgctcg actgctgcgc
    14521 aaaatggcgt cgctctccac gttgtcgcgt aagggtgttc agcgctggga gcaggccgac
    14581 gccgagccgg agagtcacgc aggcgctgtc ctcgtgagtg gaccggcgtt ctgcccgcgc
    14641 gcagccgatc gaaaggacca tcgaaaggac cattgcttga aggccaatcc gcccagccct
    14701 ctgcctgatg cccatcgggt gctgcgcgag agcgaggagc cgtaccgttc gctgttcgag
    14761 cacatgctcg atggctgcgc ttactgccag atgctgttcg atgagcaggg ggggccagat
    14821 gatttcgttt atctcgccgt gaacgaggcc ttcggaagac tgactggttt gaagaacgtc
    14881 gtcggcaagc gggccacgga ggtgattccg cgaatcaagg agctgaaccc ggagctgttc
    14941 gagatttatg gccgtgttgc ctcaacgaac aagccggaaa gattcgagat cgactttaag
    15001 cctctggggc tgtacctgtc ggtttcggcc tactgccccg agccgggtca ctttgtcgcc
    15061 gttttcgatg acatcagcga gcgcaaacag gcggagctgc gcctgcgcca ggccaaggaa
    15121 gaatgggagc gaacattcga ctccgtgccc gacccgatcg cggtgctgga ccgtgcccac
    15181 cgggtgcggc gggtaaaccg agcgatggcg gaccgcctgg gcgtcacgcc ggaacagtgc
    15241 gtagggctgc attgccatga ggccgtgcac ggcttgggtg cgccacccgc cttctgcccc
    15301 cacatgctca cctgtgcgga cggccagacg cacacggtcg aagtgcacga ggagcggctc
    15361 ggcggccatt tcctggtcac gaccacgcct ttacgcgacg agcaggggcg cgttttgggc
    15421 tcggttcacg ttgcacgcga catcaccgcg cgcaagcggg cggaggaggc gctacgcgcc
    15481 agccacgagc ggctgaagaa ggttttggag gtcgagacgg tcggcgtgat gttctgggac
    15541 ctgaagacgg ggtacctgac agacgccaac gacaccttcc tgaagatgac cggctacagc
    15601 cgtggcgacc tggaggcgca cctcctgagc tggcaaaagt tcgccccgcc ggaataccaa
    15661 gaggttggcc tggccgggat ccgaaagttc caggcgaccg ggcgcgcggg tccatacgag
    15721 aaggagtgtg tccgtaagga cggcacgcgc cagtggctcc tttttgccgg cagttcgctg
    15781 ggcgacgaca cctgcgtaga attctgcgtg gacatctccg cgcagaagaa tgccgaggcg
    15841 gcgctgcaaa aggcgcatga cgagctggaa aagcgcattg cggagcggac cgcacaactg
    15901 aatcagacga ccgagttggc cagggctgaa cggcagcggt tctaccacgt gctcgagacg
    15961 ctgccggctt acgtctgtct gatgacgcca gactaccgga tagcatttgc caaccggata
    16021 ttccgcgaat ggttcggcca ttctttgaac aggaaatgct acgaattcct gtttgatcac
    16081 gaccagccgt gcgaacgctg ccaaacgtac tccgttctga aaaccaacgc gcctcatcgt
    16141 tgggagtgga ccgggccgaa tgggcgaacc tacgatgtat tcgatttccc gttcatagat
    16201 accgacggcg cgcggctcat tctcgagatg ggcattgaca taaccgagcg caagcaggct
    16261 gaaaaagcgc tccaggaagt taacgagacg ctggaacagc gtgtcgccca gcgcaccgca
    16321 gagttgcgcc agagccgcga ggatctcgat cgcgctcaag ccgtgggcca gatcggctgg
    16381 tggcggttgg acacgcgcaa aaacgtcctc acctggtcgc ccgagagcca tcgcatcttc
    16441 ggtgtgcccg agggcacgcc gatgtcctac gagtcctttc tgtcgaccat tcatcccgac
    16501 gatcgtcagt acgtggacgc gcggtggcag gcggccctgc gcggtgagcc gtacgacctc
    16561 gagcaccgca ttgtggccaa cgggcacgta aagtgggtgc gtgagaaggc ctacctcgag
    16621 ttcgattccg accgcaaact cctgggcgga ttcggcatca cgcaagacat caccgcccgt
    16681 aaaacggctg aggaggcatt gcgccggagc gaggagttcc tgcgccgcgt gatcgacacc
    16741 gtcccgagca tgattttcat caaggactgg gagggccgct tcgtcctggt gaacgaggcc
    16801 ctggcccgga catacggaac gaccacggcc gagatcatcg gcaagacgga tgcggacttc
    16861 aacgccaaca cggaggaggt ggagcacttt cgtcgcgacg atcgcgaggt catgagcacc
    16921 cgctgcacca agattatccc cgaggagccc gtcacccacc ccgacgggga agtccgctgg
    16981 tacagcacgg tcaaagtccc gctggtcaag cccgacggca ccagcgcctg ggtgctgggc
    17041 gtggcctctg acatcaccgc tcgcaagcag gccgaggagg agctgaaggc cgccaaggtc
    17101 agcgccgagc aggcccgggc catcgccgaa tccgccaaca aggccaagga ccagttcatc
    17161 gccgtgcttt cgcacgagct gcgcaccccg ctgacgcccg cggtggccgc gctgtcgctg
    17221 ctgcgaaccg actcgcgcct gccgtccgat gttcatgaag acctggagat gatcagccgc
    17281 aacctcaatc tcgaagtgcg gctcattgcc gacctgctgg acgtgagccg catcatctcc
    17341 ggcaagctgc acctggataa gcgtccggcc gacgtggcgg tggcgatccg cgaggccgcc
    17401 aagatcgtca gcggcgacct ggacgccaag ggccagacgc tcgcgattga aacgcccggg
    17461 gcgccatacc tcacgctggc cgatggcgcc cgcttgcagc aggtgttctg gaacctgctg
    17521 cgaaactcga tcaaattcag ccccgaacga agttgcatca ccgtgcgcgc acgccttacg
    17581 ccggtcgaag gctgcccgat ggccgcccaa ccttgcccgg tcggccgggg agactgcccg
    17641 gccgcgcctt ccaccgccgg cgaccaagag ccgcggcccg cgaacctggt ggtcgaggta
    17701 atcgaccagg gcagcggcat tcatccggac atgctgccgc ggctgttcaa cccgtttgag
    17761 caagaccaca aggcccgcag cttcggcggg ctgggactgg gactgagcat ttgccgcgca
    17821 gtagtcgaga tgcacggcgg gacaatatct gcccacagcg agggggtcgg ctgcggagcg
    17881 acgttcacgg ttcgcctgcc aatcgcgcaa tgccagcgcc ccagccccag cgagcagccc
    17941 gtgcggaacc catcgggcga ggcggccgaa acgcggccgc gtgaccgggc cttgcgcatt
    18001 ctgctggtcg aggaccacgc ggataccgcc aagctcatgc gccggctgct gatggcggaa
    18061 ggatacgagg tggtcacggc cgggagtgtg gccgaggcgc tggcggcggc ggagcgccac
    18121 cgtcccgacg tgctgatttc tgacctggga ctgccggacg ggagcgggct gaacctgatg
    18181 gcacagctca tcgcccgcgg tcagcatatt ccgggcatcg cgctgagcgg atacggcacg
    18241 ccggcagact tggagcagag ccgggctacg ggattcgctg agcacttggt gaagccattg
    18301 cacagcatgg atgtcgttac cgcggctatt gcgcggctga acttaaacgc ataatccagc
    18361 cgccggacgc actgagccgc aaggcaaccg ccaagatgca aagagcgcta agaccgccaa
    18421 gaaaaagtta gttgcggcgc gtgcgggctc gccgttcgtc ctcaggcatg ccctccaatt
    18481 ccttctcgcc agagtggggt cgtaccacgc ccgcagccga tacgtaccat tggtcggggc
    18541 ttctgcgccc gcgaaccatg agtgcgccga gcgccggaag ccatttgacc tgaaacagtt
    18601 gagctgccga cccttttctg cgccgtgtta aggaggtgat atgccggggc gagtttcgac
    18661 gggcgaaccg ccgcgcgttc cagccgtggc gtcggcaagt gtgcctactg cccaaccgct
    18721 ttcgccccag gatagttgtc tccgggtgcc gccggtgcgc ctgtcggcgg cgcattcgcc
    18781 acgctcggcc gttctcacgc ccgctgcggt cctgctgctg ctcgtactgg ggctgacgtg
    18841 catgaagcgc gccctcattc acccactgcg gaactatctg ctcgattttc gctcctacta
    18901 cgcgagtgga ttggcgctgc gggccggcct gaacccgtat gacacggccg ctgtcaagtc
    18961 gtttgttcag ctccctgggc ggcagagtgc gccgccttac gtctattcgc cgccgacgct
    19021 ggcactgtgc gcactggcgt cttacctgcc atatccctcc gctcagacac catggctgct
    19081 gctgcaattc gcgctggccg gcgctgcgtt tgtgctcaca ctgcgctggt tgcgcagacc
    19141 gtggtattcg caagctgccg ctttgacggg ctggtgttcc tcttgagccg tcctttcagc
    19201 gagcttttcc gctggggcca atgggatatg tt
//
LOCUS       DAOSYS010000040        19202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2354_length_19202_cov_5.793388, whole genome shotgun sequence.
ACCESSION   DAOSYS010000040 DAOSYS010000000
VERSION     DAOSYS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 19202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19202
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_19202_cov_5.793388"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLU99_04010"
     CDS             <1..385
                     /locus_tag="PLU99_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA cytosine-C5-methylase"
                     /protein_id="HOO16253.1"
                     /translation="RPAPTRKRDDLGEFDAALVDVPCSNTGVVARRPEARLGLTSEKL
                     ASLIKTQRELIETAARHIRPGGRLVYSTCSIEPAENQQVVAWFLARHPHWLLDREQTT
                     LPAWGPRLADWRDGGYAARLLRRAD"
     gene            complement(426..1142)
                     /locus_tag="PLU99_04015"
     CDS             complement(426..1142)
                     /locus_tag="PLU99_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOO16254.1"
                     /translation="MPNTARNSPSRRDRILILGAGGHGRVVLDILLQAANYQVVGYLD
                     NNRDMIGRRVDGLPVYGTIEDLEQFARELDIQGVIIAIGDNGTRRGLARQVDAAGIPL
                     VNAVHPAATLASTVTLGRNDVICAGVVVCDRCQIGDSVILNTGCVVDYQTMLGEGVHI
                     CPGVRIGGRVKVESGAFVGIGATVVPKVTIGCESITGAGAVVTADVPSMATVVGVPAR
                     PVKLASASEDLAAMLLPARP"
     gene            1607..3235
                     /locus_tag="PLU99_04020"
     CDS             1607..3235
                     /locus_tag="PLU99_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOO16255.1"
                     /translation="MDYKPALGAAFRLAACFGLLPALTGCALLNGFLDPTAVGHFPVD
                     SEERCIRRVLTPREPPLGLANAEEPTAADLVPHFDDYRVTAGDVVAIVIPDLNTPGQV
                     EQVVVEVSFSGNIRVPLLGSVKVVGLSEPEIEAELNARYREAGLLPQPDVRVYAQTRR
                     ARTFYVRGAVGAPGQYQITVPDMRLLDIVGVIQDIGATVTKFYVIRKQRDGAGAVCPT
                     PVLPPGEGYRPEGLILEPPPIEEGFQVNFASAAGRGWQPPLEPPPAQSAPAGSPATEP
                     VIPRSELEALLAPPEPPPPVVAEPQPKEAPFAPLVFDPKTGELIEAPARPAPPGEIGS
                     PARTVPPVAAPSPPWPEAQPGDFDWENIPELELEQRVIEIDARALFAGDPAYNIVIRD
                     GDVINVPVPTAVYYLMGEVARPGVYAFNGRDITIKQALASAGGFAPFAWPSRAEVIRH
                     QPGSDKQITISVNLDAIFANLQDDFYLRDDDIVNVGTSIVAPFLYVIRNSFRFTYGFG
                     FVYDRNFADKDAYGQKVNPQITAEARRQRRGLPF"
     gene            3235..4224
                     /locus_tag="PLU99_04025"
     CDS             3235..4224
                     /locus_tag="PLU99_04025"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOO16256.1"
                     /translation="MSQASALRTSSESARLISFDRAAQVRTALLAAAFVAVFWEQLDF
                     IPPKLGALVYRWVNDADWNHGPIIPLFSAYLCYLRWDQIRSCAVKHVWLGLPIMLVGL
                     GLYTCSLAGLLVGYAQPLGMMICLLGIVVFLCGLPVLRYAWLPWLYLFFAIPIPPRQY
                     FMLTDPLQRLAAMVACGVLNLMPGVHIDRTGTMIQPFLNAAPLPPLSVTDACSGMRST
                     MVLCALGVAVAFMAWRPWWQRVILIGSCVPIATFCNFLRVTSTSILHIYVDPKYATGT
                     YHTALGLVMIVLATFLFLGIGWVLNNILIEEGPEGAGNMSEPQPRGQTRLRHD"
     gene            4217..5056
                     /locus_tag="PLU99_04030"
     CDS             4217..5056
                     /locus_tag="PLU99_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16257.1"
                     /translation="MTSAPTTEQQMAARPAGGQRRLSRAGIRYYICLSLLLASAVGMQ
                     SAASYYKIYFRKEAVPLKRRFTELDWRKLASRYQPHPIQPRLLPPEQEEALGTEDYLE
                     LRLIDTEKPARDPAALANVFLTYYTGQPDMVPHVPDECYLAGGYERIGWATEELRVVG
                     ARAPDDRVPVRVVQFQSRSGSGEPISVIYFFSVNGEYRNGREGVRFRLARPFERYAYY
                     CKIELSFTDNENPLRPRRADMQQSLAAAQDLLTKLLPVLIEDHIDWDPARNGPAQADE
                     RSG"
     gene            5069..9958
                     /locus_tag="PLU99_04035"
     CDS             5069..9958
                     /locus_tag="PLU99_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16258.1"
                     /translation="MARKRVNKNLVAFLTVMGILLTVVVVLIATALSANQDPEIWATQ
                     ARERLATEEYQLAADLFVRAYAKSKDVKYLVEAADCYYRAGRPNALFPLLTTARNASD
                     RGAPGHLEAVKAYLKYAWNLAEPYRYWQLDPEWRSNLLQCAQDLQQADAEDPLGSVTE
                     AAARLAMAAEDPQERVQGEAALQRAISLNENAPWVVLTRIQHELHETEARLARLVQER
                     APLAEREKLEADFNRQAREWLSAAVAANPTERKLITEYVGLLQSEKKVAEARAVLDRA
                     LSQAPEAADIHATMANFLLWQARDARSELSAEEYAGLLDEAVKHSVEATRLSPGMFQA
                     YIAQVQAELMRDAREANAPIPRERYETALRICEHALQSTVGIQSIDAWRGARLYQEAL
                     LHIQAFNTAVQYLVSPGQAADREQRLEWVRKFREAIEARYPDQAFTWYVRGLAHMTEN
                     DAGNAIQAFENAKRIEQANPRVALYLWHHGFNGILPVHRLAMLYRQLNEPGIALNLTE
                     EALQRYAQLYPGQAPPMELCISRAELLGTLNRSQDALDYIQELRHEYAAVLNDSDRER
                     LAFAEAQALSLSQRGAEAASALPKADTPASLLMQAKLALVAQDRETAAARLQALLSAA
                     DVQPQQVIEGLGLLAPLLTEPEQRQQLRPELAELRKRFAGNAALLRRLDQYDAVLTYT
                     DPEQLDQKLLELIAAEPEGPERARHYFDFYVARERYAEAQKYLDQMEAAGHADPTVLG
                     RQFELAVRNQDFQRAEQYAARLARANDGAGADNAGGAVFRGQLALARDRAEDAVREFR
                     AAQALLPRSSTLQVQLGQALLAANRVDEALEALREAYDLNPNNFTANVLLDDIYYRRV
                     SEDRRPDDWEKHLEQAARLRPRNQYVVENADRADPQAGIELREQRRAAEPQNVENLIR
                     LAQLYANKRIGDVERAAERLQEAARLQPGSLEVARAAAQLFGAHGQRAAGEQILGACL
                     EQHSGIERVPVQVLLARFYNLLGDTAAAEQAFATARQMALEQPAQPGEPNDVLLGVEM
                     ELVNFYRALPGRARDTITAARWVLDKLNAKADKDLPRVQFARQAIIRALLELRQLGEA
                     EKEINNYLENFGDDVAGALLQAQLKLAQRQWQEAYAALTRVLQKVPNHSGALLQRGGL
                     AIRLGLGGEAAADLMKAHDAAKAALERVPAEKRAQSSELKLYIAACAELATSYEIEEQ
                     YELGESMLRHIIDLYGPEHSAQQRETVERLLRLYERWGRPEKAQQVISEFMARHADDP
                     FWPARLAQARSQQAEKYIQQAEAAAKLDNRADEKKYRTAAESEYLAAARYYENAQQLA
                     AAQKQPGHLMYVAYQLDALAAAKRTDRALELFRNLEGQPGIVVPPIVYAAMIRVYEDS
                     GQRPQALAALERAIVSAGREGGGALPAVMGFAGDHLPADSVASVIKGLIEKVPSESRE
                     SFLLHNLLATQLLDMNQAAEALAAVEPVLAKAPPNAPVRLAAMLARQQALLASGQVDA
                     ALAALEEIVRLNPNNALVLNNLAYLLVDSGNRPAEALPYAERARELAGSDPSVLDTVG
                     WVYARNGRNEEAEAALKEALAQDPHSAITNYHLGVLYQTMGRNTAARIALQRALENAA
                     RQKNEELQRKAQEALNELH"
     gene            10026..12278
                     /locus_tag="PLU99_04040"
     CDS             10026..12278
                     /locus_tag="PLU99_04040"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOO16259.1"
                     /translation="MSTMTDAQGIVRRAPYSPAWPPAGEPQAPTEETGLTAADIIRAL
                     KQRKLLLVIMFAVLYALVLATTVAVWKWLPGFTANALLQLHPPQEDPLMPAEALVAPN
                     IMDLLVQTEAMRIKRPDILQDVLSLPEVKATNFYKWYSSFDKAVEDLQDLIRVSPIPD
                     TMLISLSLSCRDRDEAVLIVKKLAERATQSYREEAGYRGQQALEDLKRTRDTVKRELE
                     QRQRELENFRKGHDVGAIETEATTLGQKIADQNWAVNSYMARQAELQAQLNIVGGRDP
                     RQLPITPEDRVIVEADPLLRLYRQQVESLDIRINAAKKHTIGENNRFMKELLAQRDGY
                     YERERARREELLDDLRERRVQSIREELARVQAVLGTLLEQLETYEARLATVDNARVKY
                     EAMVKEEERIQKNLEGLEEKVTQQQHAAGSEQRRARLTIVQEPKRPVWPSRPNLPVYL
                     GGGLLLSLLGAVGFVFLREVTDKYIRTPIDVARYGRLSVLGCIPALDDEEAEVEEIEK
                     ATRQAPHSLVSEAFRQVRANLLFSGPHESQHSVLITSPGAGNGKTATAINLAVTLAQA
                     GERVLLIDCNFRRPGLRRAFPELRPEGLSNILVGQGKAEDLIYQSSVPNLDLLGSGPM
                     PPTPSELLGSRYMQELIALVKSKYERVILDGPPALLVSDALVLSRLVDGVILVAQASE
                     NTKGALRRAREQLLRINARVQGAILNGVRARPGGYYREQYREFYEYGADETIPPELPG
                     PFNVGTSDPK"
     gene            12597..13367
                     /gene="truA"
                     /locus_tag="PLU99_04045"
     CDS             12597..13367
                     /gene="truA"
                     /locus_tag="PLU99_04045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOO16260.1"
                     /translation="MQRNLKLLIAYDGTDFHGWQAQPGMRTVQEELAKTLERVLRHPV
                     TLAGASRTDAGVHARGQVASLRTEAAIPAANLQRAVSCRLPPDIALVRVAEAAPHFHA
                     TRSARGKLYRYALYNSERRPAEAMAARYAWHYWHALDLDAMRAAAAVLLGTHDFAGFA
                     NQGQPRETTIRTLRSIAVRRHYERVHIEVEGDSFLYNQVRIIVGTLIEIGRGHWPVER
                     TAAILEARDRSLAGPTAPAHGLTLEWVRYDPPRSCHES"
     gene            13357..14088
                     /locus_tag="PLU99_04050"
     CDS             13357..14088
                     /locus_tag="PLU99_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO16261.1"
                     /translation="MNLEGGVVLVTGAARRVGRAIALRFASAGCGIAIHYLHSGEEAQ
                     RTADQCRALGVTAETFRADLNDPVATAALVPEVLERFGRLDVLVNNASVFEPMTLDTF
                     SPAEWERTLRINLTAPMILAHAARAALEQAGGRIINLADAAVAHPRPEYLAYVVSKGA
                     LETLTQVLARALAPRVNVVGIAPGVAVFPEHYDAEMRKRLTSRIPLQRTGTAEEVAEL
                     AEFLVRAGDFITGVVIPIDGGRRLL"
     gene            14198..16105
                     /locus_tag="PLU99_04055"
     CDS             14198..16105
                     /locus_tag="PLU99_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16262.1"
                     /translation="MCCSWKLPDLVRKGAPRRVLLWCAATFVFTPIWASAQVPITQDG
                     RLFDANTQISGSRYNFGRPQSPLLLGNSVATGNVRSGLSFRGFQPISDPTSFMTTIPS
                     GALSNFRRDSVSVADAALASPLIGGPWYAPSLTAPTVGFLSGQFLPRTPIPDQRLQPA
                     RDSGIMTALRQPLGPIGPPPSAVRGSSELLSAQIIGMIPGELTSSIFGPPQPPAATPT
                     RETFTPSVVEPGLRGGQATIGQTGINWTAPYLQEVPGSRPPLGTMLDLVRERRLQRFP
                     VDTEQEPGTPGELLPQPQPQPQLPSSLGPVMPEPEQSDASPLTFGPGALAERFGESRT
                     LRPGAMDSAGADLAGISAPLPDTSVLPGYDVFTDMQLAMALSGNPNADWFAQMQRAIR
                     ENPATAVMLRERADVDAQEFIQQMLNSPIQTFHGRGESPKNNEMLRAESLMEIGHYFE
                     ASRRYQIAHRIDPLDPLPLIGRGNALLAAGEYLTAALALMQGFERYPELSRFALDLPK
                     LVGGREIIDIRRADIMRMLETRDDPQLRFLLGYLEYFSGDAKLRESGLANFDRAAHLD
                     TSNSLISRMPALLRHEGTLPPPKLPEQDSFLPEEIPTPTELGAPDQTSPPPPVPLDPP
                     QPRPLETGEPQ"
     gene            16165..16680
                     /locus_tag="PLU99_04060"
     CDS             16165..16680
                     /locus_tag="PLU99_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16263.1"
                     /translation="MAATNQQSRLIQECQPDKVMTVAARMLQREFGRVRVNCEALTIE
                     TEPAEFLTTCDSGTARDLYRGTSTMRRVAHFSLARSGSGTLARLRIDVERRDTERVAN
                     ALMPTQTLGDLPTVSATPIQREAATTERQNTVWTFVRRDRQMERCLLDELASEFAPPA
                     VEPPALEAPGS"
     gene            16735..17994
                     /gene="glyA"
                     /locus_tag="PLU99_04065"
     CDS             16735..17994
                     /gene="glyA"
                     /locus_tag="PLU99_04065"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOO16264.1"
                     /translation="MLKFMSQPLAETDPEVAGIIRREEERQQNTLELIASENHVSVAV
                     RTAVGSVFTNKYAEGYPGKRYYGGCTNMDSVEDLARERAKKLFHAEHVNVQPHSGAQA
                     NTAVFMACLKPGDTVLSLDLAHGGHLTHGLKVNFSGTMYNIVPYVVSPKTEMLDMAEL
                     RRLALEHKPKLIIAGASAYPRRLDFPAFAEIAKECGARLMADIAHIAGLIATGLHPTS
                     IGFADFTTTTTHKTLRGPRGGMIMCGAADAALVDKWVFPGTQGGPLMHVIAGKATAFG
                     EALRPEFKEYQQQILSNAQALAEALRARGYRLVSGGTDTHLMLVDLRDVHPDLTGKQA
                     QDWLEEAGIITNKNMIPYDQRKPTQTSGLRLGTPALTTRGLKEPQMAVVAELVHQVLL
                     AKGDQAAMQRIRGQVRDLCTQFPVPHA"
     gene            complement(18175..>19202)
                     /locus_tag="PLU99_04070"
     CDS             complement(18175..>19202)
                     /locus_tag="PLU99_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16265.1"
                     /translation="PDLDPHRIPGRIDINTAPWYVLAGLPVINPDLTDPNKIQIDHTA
                     SPAFWSPVCGILTGVVDEFETPEVVGLPGGALYRFGPATAPPAESPNDLLVVDQTEPY
                     WIRLGPYYAQAIVSYRDQLRYASDPGTLSSGRNPLWAADLRNASIDSDGTTSPAVEAQ
                     YRLEVASIPGDGLGGFQYGAIRRGDLKHGFLSLGELLNVKGFDCPVRHAHSGWRGLRR
                     STDLLYNPLGQPNDGRKMNCPDFFKAVSLMALLDTHFLTTRSNTFTVYMTLMNRDPEQ
                     QHSSIRVQYTVDRSNLLPRLATDEEGRPLMQATQDGEVPVILRRDGLPEIITRREAGY
                     YDMRYDQ"
BASE COUNT         3600 a         5983 c         6305 g         3314 t
ORIGIN      
        1 acgcccggcc cccactcgca agcgcgatga cctgggcgaa ttcgacgcgg cgctggtcga
       61 tgtgccctgc tccaataccg gcgtcgtcgc ccgccgcccc gaagcccgcc tcggccttac
      121 tagtgagaag ctcgcctcgc tcatcaagac gcagcgggaa ctgatcgaaa ccgcggcccg
      181 ccatatccgc cccggcggcc gtttggtcta cagcacctgc agcatcgagc cggcagagaa
      241 tcagcaggta gtagcttggt tcctggcccg tcatccgcat tggctgctgg accgcgagca
      301 aacgacgttg cccgcctggg ggccgcggct ggccgattgg cgcgacggcg gctacgccgc
      361 gcgactcctc cgccgggcag attgatccgc agctcccggt atgccggctg ttccggaccc
      421 ccggatcaag gcctggcggg aaggagcatg gcggccaagt cttcggaagc gctggcgagt
      481 ttcaccggcc gtgcgggaac gccgacaacc gtcgccatgc tggggacatc ggcggtgacc
      541 acggcaccgg cgccggtgat tgattcgcag ccgatcgtaa cctttggaac gaccgtcgcg
      601 ccgatgccga cgaaggcgcc ggactccacc ttaacccgtc cgccgatgcg tacaccgggg
      661 caaatgtgga ccccctcgcc gagcatcgtc tgataatcta cgacacagcc ggtattcaaa
      721 atgaccgaat cgccaatttg gcaacggtcg cacacgacga ctccggcgca aatcacgtcg
      781 tttcgcccaa gcgtgacggt tgaggccagc gtggccgccg gatgaaccgc attgacgagc
      841 ggaatacccg cggcatccac ttggcgtgcc aggccgcggc gcgtgccgtt gtcgccgatg
      901 gcgatgatga cgccctggat gtcgagctca cgggcgaatt gttcgaggtc ttcaatcgtc
      961 ccgtataccg gcaggccgtc gacccggcgg ccaatcatgt cgcggttgtt gtcgaggtag
     1021 ccgacgacct gatagtttgc ggcctgtagc aggatgtcga gaacgacgcg cccgtgtccg
     1081 ccagcgccga gaatgaggat gcgatcgcgg cggctcgggc tgtttcgggc agtattcggc
     1141 atgccgtggt cccgttccaa tcaagggcgc ttattgccag acgaactttc gccaacggtc
     1201 ttgtgaatcg tggccgaacg cggcgagcgc aaccgtccta tcggaaaaca gggagggaac
     1261 gatcccgtcg gcgcctcgaa tagcgcacta gggagcggtt ttgcccattc gggcgagcgt
     1321 cccgggcacg gccgaaagaa ccgagcgtgc gtgagccgat aaacagtgcc gggaagccga
     1381 gacgaggatt gtgaactgtc tggccgcgcc cgacgtaagt tgtttgccgt gcgctgaata
     1441 ggtttgggat tgtcgccgtg ctcgtttgac aactttcggg taacgccagt aaattgctca
     1501 gttcctcgag cgagtccgag ggcgtgatca ttcagcctta agccgaagat gaaactcgag
     1561 ttggaccgga caactgccgg caccagcgcg agcggaggaa tgcggaatgg attacaagcc
     1621 ggcgctcgga gcagccttcc gcctggccgc atgcttcggg ttgctgccgg cgctcactgg
     1681 ctgcgcgctg ctaaacggct ttttggaccc gaccgccgtt gggcattttc cggttgattc
     1741 ggaggagcgc tgtattcggc gcgtcctcac gccgcgtgag ccgccgctgg gattggcaaa
     1801 tgcagaggag cccacggcgg ccgacttggt gccgcacttt gatgactacc gcgtgacggc
     1861 gggcgacgtg gtggccatcg taatcccgga tctaaacact ccagggcagg tcgaacaggt
     1921 cgtcgtcgaa gtcagcttta gcggaaacat acgcgttccg ctgctgggtt ccgtcaaggt
     1981 agttgggcta tccgagcccg aaatcgaagc cgagttgaac gcgcgttatc gcgaggccgg
     2041 gttgctcccc caacctgacg tgcgcgtgta tgcccagacg cgtcgcgcgc gcaccttcta
     2101 cgttcgcgga gcggtcggcg cgcccggcca atatcagatc accgttcccg acatgcggtt
     2161 gttggacata gtcggggtca ttcaggatat tggcgccacc gttacgaagt tctacgtgat
     2221 cagaaagcag cgtgacgggg caggcgcggt ttgtcccacg ccggtgctac cgccgggcga
     2281 ggggtaccgc cccgaaggtc tgatcctgga accgccgccg attgaagagg gtttccaggt
     2341 caacttcgcg tcggccgcgg gacgtggctg gcaaccgccg cttgagccgc cgccggccca
     2401 aagtgcgcct gccggatcgc ctgcaaccga accggtgatt ccgcgctccg aactcgaagc
     2461 cttgctggct ccacccgaac caccaccgcc cgtcgtcgct gagccacagc cgaaggaagc
     2521 gccttttgct ccgctcgttt tcgatcccaa gacgggggag ttgattgaag caccagcgcg
     2581 cccggcgccc ccgggggaaa tcggatcgcc ggcgcgaacc gtaccaccgg tcgccgcacc
     2641 cagcccgccc tggccggaag cccagcccgg cgattttgac tgggagaaca tcccggaact
     2701 ggagttggag cagcgcgtaa tcgagatcga cgcgcgcgcg ttgtttgcgg gcgatccggc
     2761 gtacaacatc gttattcggg atggggacgt gatcaacgtg ccggtcccga cggcggtgta
     2821 ctacctgatg ggtgaagtag cgcgcccggg cgtttacgcc tttaacggcc gcgatatcac
     2881 gatcaagcag gcgctggcca gcgccggtgg atttgcgccg ttcgcgtggc cgtcgcgcgc
     2941 cgaggttatt cggcaccagc cgggctcgga caagcagatc acgatttcgg tcaacctgga
     3001 cgcgattttc gcgaacttgc aggacgattt ctacctgcgt gacgacgaca tagtgaacgt
     3061 ggggacgagc attgtagcgc cgtttctgta cgtgatccgc aattccttcc gtttcaccta
     3121 cgggttcggg tttgtttacg accgcaattt tgcagacaag gacgcatacg ggcagaaggt
     3181 caatccgcag attacggcgg aggcgcgccg ccagagacga gggttgccat tctgatgagc
     3241 caggccagcg cattgagaac cagcagcgaa tcggcccggc tgatcagctt tgatcgagcg
     3301 gcccaggtcc ggacggcgct gctggcggcg gcgtttgtcg cggtcttctg ggagcaactg
     3361 gatttcatcc cgccaaaact gggagcgctc gtctaccgct gggtcaatga cgccgactgg
     3421 aaccatggcc caatcattcc cctgttcagt gcgtatctct gctatctgcg ctgggaccag
     3481 attcgaagct gcgccgtaaa gcacgtgtgg ctcgggctgc cgatcatgct ggtggggctg
     3541 ggattataca cctgctcgct ggccggcctg ctggtcggct acgcgcagcc gctgggaatg
     3601 atgatctgcc ttttggggat cgttgtgttt ctatgcggct tgccggtgct gcgctatgcg
     3661 tggctgccgt ggctgtacct tttctttgcg attccgatac cgccgcggca gtatttcatg
     3721 ctcaccgacc cgctgcaaag gctggccgcg atggtggctt gcggcgttct gaacttgatg
     3781 ccaggcgtgc acatcgatcg aacggggacg atgattcaac cgtttctgaa cgccgccccc
     3841 ctgccgccgc tgtcggtgac ggatgcctgc agcggcatgc gcagcacgat ggtactgtgt
     3901 gccctggggg tggcggtggc gttcatggcc tggcggccgt ggtggcagcg cgtgatcctg
     3961 atcggatcct gcgtgccgat agccactttc tgcaacttcc tgcgcgtcac gagtaccagt
     4021 atcctccaca tttacgtgga cccgaagtac gccaccggca cgtatcacac ggccttaggc
     4081 ttggtgatga tcgtgctggc cactttcctt ttcctcggca tcggctgggt gctgaacaac
     4141 atcctgatcg aggaaggacc cgaaggagcg gggaacatga gcgagccgca accccggggg
     4201 cagacccggt taaggcatga ctagcgcgcc gacaaccgag caacagatgg ccgcgcggcc
     4261 cgccggcggg caacgccggt tgagccgggc cggcattcgc tattacatct gcctttcgct
     4321 cctgctggca tcggccgtcg gcatgcagag cgccgccagc tattacaaga tttactttcg
     4381 caaggaagcg gtgcctctga agcggcgctt caccgaattg gactggcgca aactggcctc
     4441 acgctatcag ccgcacccga ttcagccccg gctattgccg cctgagcagg aagaagcact
     4501 ggggacggag gactatttgg agctgcggct gatagatact gagaagccgg cgcgcgatcc
     4561 ggcggcgcta gccaacgtgt ttctcacgta ttacacgggg cagcccgaca tggttccgca
     4621 cgtgccggac gagtgctatc tggcgggcgg ctatgagcgg atcggctggg ccaccgagga
     4681 actgcgcgtc gttggggccc gggccccgga tgaccgggtg ccggtgcggg tggtgcagtt
     4741 ccagtcgcgc agcgggagcg gtgagcccat ctcggtgatt tactttttca gcgtcaacgg
     4801 tgagtaccgc aatgggcgtg agggtgttcg tttccgcctg gcgcgcccct ttgagcggta
     4861 cgcctactac tgcaagattg agttgagctt cactgataat gaaaacccgt tgcggccgcg
     4921 gcgggccgac atgcagcagt cattggcggc agcccaggat ctgctgacga agctgctgcc
     4981 ggtgctgatc gaggatcata tcgattggga cccggcgcgc aacggtccgg cgcaggcgga
     5041 cgagcgcagc gggtagaagg tgactggcat ggctcgcaaa cgagtgaaca agaatctggt
     5101 ggcctttctg acggtcatgg gcattctgct gacggtcgtg gtggtgttga ttgccacagc
     5161 cttgagtgcc aaccaagatc cggagatttg ggccacgcag gcccgggagc ggcttgcgac
     5221 cgaagagtat cagttggcgg ccgatctgtt cgtgcgcgct tacgcgaaaa gcaaggacgt
     5281 caaatatctg gtcgaggccg ccgactgtta ctatcgcgca ggccggccta acgcactctt
     5341 cccgctgttg acgaccgccc gcaatgcctc ggaccgaggg gcgcccggac acctcgaagc
     5401 agtgaaagct tacctgaaat acgcctggaa cctggcggag ccctacagat actggcagtt
     5461 ggatcccgag tggcgctcca atctgctgca gtgcgcgcag gacttgcagc aggcggacgc
     5521 cgaggatccg ctgggcagcg taactgaggc cgccgcgcgg ctggcgatgg cggcggagga
     5581 tccgcaggag cgggtgcagg gcgaagcggc gctgcagcgc gccatcagcc tgaacgagaa
     5641 tgccccctgg gtggtgctga cgcgtatcca gcacgagttg cacgagactg aggcgcgcct
     5701 ggccaggctg gtccaggaac gcgccccgct cgcggagcgc gagaagctcg aggcggattt
     5761 caaccggcaa gccagggaat ggctgagtgc cgccgtagca gccaacccca ctgaacggaa
     5821 actgattacc gaatacgtcg ggctgctgca atcggagaag aaggtcgcgg aagcgcgagc
     5881 cgtgctggat cgcgctcttt cgcaagcccc cgaggcggcg gacattcatg ccactatggc
     5941 aaattttctg ctgtggcagg cgcgcgacgc gcgttccgag ctctcggcgg aagagtacgc
     6001 cggcctgctc gatgaagcgg tgaagcacag cgtcgaggcg acgcggctct cgcccggaat
     6061 gttccaggcc tacatcgctc aagttcaggc agagttgatg cgcgacgccc gggaggccaa
     6121 cgcgccgatt ccccgggagc gctatgagac ggcgttgcgc atctgcgagc acgcgttgca
     6181 gtcaaccgtc ggcatacaga gtatcgacgc ctggcgtggt gcgcggctgt accaggaggc
     6241 cttgctccac atccaagcgt tcaataccgc cgtgcagtat ctggtcagcc cgggccaggc
     6301 cgcggaccgt gagcaacggc tggagtgggt acgaaaattc cgcgaggcca tcgaggcccg
     6361 ctatccggat caggcgttca cctggtacgt ccggggcttg gcgcacatga cggaaaacga
     6421 cgccggcaac gccatccagg ccttcgagaa cgccaagcga atagaacagg ccaacccgcg
     6481 cgtggcgctc tacctctggc accacggctt caacggcatt ttgccggtcc accggctggc
     6541 gatgctatac cgtcagttga acgaaccggg gatcgcgctg aacctgaccg aggaggcgct
     6601 gcagcgctac gctcagctct accccggcca ggctccgccc atggaactgt gcatcagccg
     6661 cgcggaactg ctgggaacgc taaaccgctc tcaggatgcc ctcgattaca tccaggaact
     6721 gcgccacgaa tacgcggccg tgctgaatga ctccgatcgc gagcggctgg ccttcgccga
     6781 agcccaggcg ctgagtttgt cccaacgcgg ggcggaggcg gccagcgccc tgcccaaagc
     6841 cgatacgcca gccagcctgc tgatgcaggc caagctagcg ctcgtggcac aggatcgcga
     6901 gaccgccgcg gcgcgcctgc aggccctgct ctcggcggcc gacgttcagc cgcagcaggt
     6961 cattgagggc ctggggctgc tggcgccctt gctgactgag ccggagcagc gtcagcagct
     7021 gcgcccggaa ttggcggaac ttaggaagcg ttttgcaggc aacgcggcgc tgctgcggcg
     7081 tctggatcag tacgacgcgg tgctgaccta cacggacccg gaacaactcg accaaaagct
     7141 gctggagttg atcgcggccg agccggaagg tcccgagcgg gcccggcact atttcgattt
     7201 ttacgtggcg cgtgagcgct atgccgaagc gcagaagtac ctggaccaga tggaagccgc
     7261 cgggcatgct gatccgacgg tgctgggccg gcagtttgag ttggccgtac gcaaccagga
     7321 cttccagcgg gccgagcagt atgcagcccg gctggcgcga gccaacgacg gggctggcgc
     7381 agataacgcg ggcggggcgg tcttccgcgg gcaactggca ttggcgcggg atcgggccga
     7441 agacgcagtg cgcgagttcc gtgcggctca agccctccta ccgcgttctt ccacgcttca
     7501 ggttcaactg gggcaagcgc tgctggcggc caatcgcgtg gacgaggcgt tggaagctct
     7561 gcgcgaagcg tatgacttga atccaaacaa tttcaccgcc aacgtgctgt tggatgacat
     7621 ctactaccgg cgcgtcagcg aggatcgccg cccggatgac tgggagaagc acctggagca
     7681 ggccgcacgc ttgcggccgc gcaaccaata cgtggtagag aatgccgacc gtgccgatcc
     7741 gcaggcaggg atcgagctgc gcgagcagcg ccgcgcggcc gagccgcaga atgtggaaaa
     7801 cctgattcgg ttagcccagc tctacgcgaa caagcggatc ggcgatgtcg agcgcgccgc
     7861 cgaacggctg caggaagccg cccgcttgca gccggggagt ttggaggtgg cgcgggccgc
     7921 agcgcagctc tttggcgcgc acgggcagcg tgctgcgggg gaacagattt tgggtgcctg
     7981 cctggagcag cacagcggga tcgagcgtgt gccagtccag gtgctattag cccgttttta
     8041 caacctgctg ggcgatactg ccgccgccga gcaggctttt gcaacggcgc ggcaaatggc
     8101 gctggagcag cccgcgcagc cgggtgaacc aaacgacgtc ctgctgggcg tggaaatgga
     8161 gctggtgaat ttctaccggg cgcttccggg gcgggcgcgc gacacgatca cggcggcccg
     8221 ctgggttctg gataagctga atgctaaggc tgacaaggat ttgccgcggg tgcagttcgc
     8281 gcggcaggcc atcatccgcg ccctgctgga actgaggcag ttgggcgagg ctgaaaagga
     8341 aatcaacaat tacctcgaga atttcgggga cgacgtagcc ggtgcgctgc tgcaagcgca
     8401 gctcaagttg gcgcagcgtc agtggcagga agcgtacgcc gctctgacgc gggtgttgca
     8461 gaaggtcccc aatcactccg gggcgctgtt gcagcgcggc ggactggcga tacggctcgg
     8521 cttgggcggc gaggcggccg cggacctgat gaaggcccat gacgcagcca aggcggccct
     8581 cgagcgtgtg ccggccgaga agcgggcaca gagcagcgag ctcaagctgt acattgcggc
     8641 ctgcgccgaa ctggcaacct cgtacgaaat cgaggaacaa tacgagctcg gcgaaagcat
     8701 gctgcgccac atcatcgacc tttatgggcc ggagcacagc gcccagcagc gcgagacggt
     8761 cgagcgctta ttgcggcttt atgagcgctg gggccgtccg gaaaaggcgc aacaggtaat
     8821 cagcgaattc atggcgcggc acgccgacga cccattctgg ccggcgcgat tggcccaggc
     8881 gcgcagtcag caggccgaaa agtacatcca acaggccgag gcggccgcca agctggacaa
     8941 ccgcgcggac gagaaaaagt accgtacagc cgccgaaagc gagtacctcg ccgcggcgcg
     9001 ctattatgag aacgcccagc agctcgccgc ggcgcagaag cagcctgggc atttgatgta
     9061 cgtggcttac cagttggatg cgctggccgc ggccaagcgg accgaccggg ccttggagct
     9121 gttccgcaat cttgaagggc agccggggat cgtggtgccg cccatcgtct acgccgcaat
     9181 gatccgcgtt tacgaggaca gcggtcagcg gccgcaagcc ctggcagccc tggagagagc
     9241 cattgtctcg gccggccgcg agggcggcgg cgccctccca gccgtgatgg gcttcgccgg
     9301 cgaccacttg ccggccgatt cggtggcgag cgtgataaaa gggttgatcg agaaggtgcc
     9361 gtccgaaagc cgcgagtctt tcctgctgca caatctactg gcgacgcagt tacttgatat
     9421 gaaccaggcg gccgaggcgc tggcggcggt cgaacccgtg ttggccaaag cgccgcccaa
     9481 cgcgcccgtg cggctggctg caatgctggc tcgccaacag gcgctgttgg cgagcgggca
     9541 ggtggacgcc gccctggcgg cattggagga gattgtccgc ctcaacccga acaacgccct
     9601 ggtcttgaac aacctggcct acctgctggt cgatagtggc aatcgcccgg ccgaggcgct
     9661 gccctacgcg gaacgggcgc gtgagctggc cggttcggat ccctcagtgc tggacacggt
     9721 cgggtgggtg tatgcgcgga atgggcggaa cgaagaggct gaagcggcct tgaaggaagc
     9781 tctggcgcag gatccccaca gcgcgatcac caattaccac ctgggagtac tctaccagac
     9841 aatggggcgt aatacagcgg cccgtatagc gctacagcgg gcgctggaga acgcagccag
     9901 gcaaaagaac gaagaattgc agcggaaggc gcaggaagcg ctgaacgagc ttcattagca
     9961 cggccggaac cgattcgggc cgcggttctg ttgcgtccag cgaggacgtt tgctacgagg
    10021 tggcgatgag taccatgacg gatgcccaag ggattgtcag acgcgcaccc tactcaccgg
    10081 cgtggccgcc ggcgggcgag ccgcaggccc cgactgaaga aacgggatta accgccgcgg
    10141 acataatccg cgccctgaag cagcgtaagt tgctgctggt gatcatgttc gcggttcttt
    10201 atgccctggt gctggcgacc acggtcgcgg tgtggaagtg gcttccgggg ttcacggcca
    10261 acgcgctgct gcaactccat ccgccccagg aagatccgct gatgccggcg gaggcgctgg
    10321 tggcgccgaa tatcatggac ttactggttc agaccgaagc catgcgtatc aagcgcccgg
    10381 acatcctgca ggacgttctg agtctgccgg aggtgaaagc caccaatttc tataagtggt
    10441 acagcagctt cgacaaggcg gtggaggact tgcaagacct gatccgggtc agtcccatcc
    10501 cggacaccat gctgatcagt ctcagcctga gttgccgcga ccgcgacgaa gcggtgctga
    10561 tcgtgaagaa attggccgag cgcgccaccc agagctaccg cgaagaagcc ggatatcgcg
    10621 ggcaacaggc gctggaggac ttgaaacgta cgcgcgacac ggtcaagcgc gagttggagc
    10681 agcggcagcg cgagttggag aacttccgga aagggcacga tgtgggggcg atcgagacgg
    10741 aagctacgac gcttgggcag aagatcgcgg accagaactg ggccgtaaat tcttatatgg
    10801 cgcggcaggc ggaactgcag gcgcagctca acattgtcgg cgggcgcgat ccgcggcagt
    10861 tgccgatcac gcccgaggac cgcgtgatcg tcgaggccga tccgctgcta cgtttgtacc
    10921 gccagcaggt cgaatcgctc gatatccgca tcaacgcggc caagaagcac acgatcggcg
    10981 agaacaacag attcatgaag gagctcctgg cgcagcgcga tggttattac gaacgggaaa
    11041 gagcgcggcg cgaggagctg ctggatgact tgcgtgagcg ccgcgtgcag agcatccgcg
    11101 aggagctggc gcgcgtgcaa gccgtgctgg gcaccctgct ggagcagttg gaaacttacg
    11161 aggcgcggct tgcaacggtg gacaacgcgc gagtcaagta tgaagcgatg gtgaaggagg
    11221 aagagcgcat ccagaaaaac ctggagggcc tcgaggaaaa agtaacgcag cagcagcacg
    11281 ccgccggttc cgagcagcgc cgggcgcgct tgacaatcgt gcaggaaccc aagcggccgg
    11341 tctggcccag ccgtcccaat ctgcccgtct acctgggcgg cggcttgcta ctgtcgctgc
    11401 tgggcgccgt gggcttcgtc tttctgcgcg aggtgactga caagtacata cgcaccccga
    11461 tcgacgtggc gcgatatggg cgactgtcgg tgctcggctg cattccagcc ctggacgacg
    11521 aagaagccga agtcgaggaa atcgagaagg ccacccggca ggcgccgcac tcgctggtgt
    11581 ccgaggcctt tcgacaggtg cgtgccaacc tgctcttcag cgggccgcac gagtcgcagc
    11641 actccgtttt gatcaccagt ccgggggccg gcaacggcaa aaccgccacc gccatcaacc
    11701 tggcggtcac tctggcccag gcgggcgagc gcgtgttgct gatagactgc aacttccgcc
    11761 gccccggcct gcggcgcgcg tttccagagt tgcgccccga agggttgagc aacatcctgg
    11821 taggccaggg caaggccgag gacctgattt atcaaagcag cgtccccaac ctggatctgc
    11881 tgggcagcgg gccgatgcct cccacgccgt cggaattgct cggctcgcgc tatatgcagg
    11941 agctgattgc gctcgttaag agtaagtacg aacgtgtcat tctagacgga ccgccggcgc
    12001 tgctggtcag cgatgcgctc gtgctgtcgc ggctggtgga tggagtcatt ctggtagcgc
    12061 aagcttcaga gaacaccaag ggcgccttgc ggcgggcacg cgagcagttg ttgcgcatca
    12121 acgcgcgcgt ccagggcgcg attctgaacg gcgtccgcgc ccgacccggt ggctactatc
    12181 gcgagcaata ccgcgagttt tatgagtacg gcgccgacga gacgattccg cccgaattgc
    12241 ccggaccgtt caatgtcggc acgagcgacc cgaagtagcg aagccgtcgc gtattagcag
    12301 agccggggcg tgtcgccccg gcgcgtgcca aacgatatct ctgccaccag tgagcagagc
    12361 cgttcgggcg tggcccaaac gatacctcta tcaccggtgt gcagagccgg agcgtgtcgc
    12421 ccggtgacgc gccaaaatac ctgcgccagc gatgcgtgcc attgactaac gttgcgtctt
    12481 ctcattacta atcactaaaa actaaaaact aatcacaaag aacaaccgag gtgtgtcgcc
    12541 ccgaactacc gccgttccac cagaattcgt cgccaaccct accgaagccg ttgcccatgc
    12601 aacgcaatct caagctactg atcgcctacg atggcacgga ttttcacggc tggcaggccc
    12661 agcccggcat gcggaccgtg caggaggaac tggcaaaaac gctcgaacgc gtgctgcgcc
    12721 acccggtgac gctcgccggg gccagccgca ccgacgccgg cgtacatgcg cgcgggcagg
    12781 tcgccagcct gcgcacggaa gccgccatcc cagccgccaa cttgcagcgc gccgtctcgt
    12841 gtcgcttacc gcctgacata gccctggttc gcgtcgctga ggccgcgccg cacttccacg
    12901 cgacgcgcag tgcgcgcgga aagctgtacc gctacgcatt gtacaactcg gagcggcgcc
    12961 ccgcggaagc catggccgcc cgttacgcct ggcattactg gcacgctttg gacctggatg
    13021 cgatgcgcgc cgcggcggca gtcctgttgg gcacgcacga tttcgccggg ttcgccaacc
    13081 agggccagcc gcgcgaaacg accatacgaa cgctgcgcag catcgccgtt cgccgtcact
    13141 acgagcgcgt gcacatcgag gtcgagggcg actctttcct ctacaatcaa gtgcggatca
    13201 ttgtgggcac cctgatcgag atcggccgcg ggcactggcc ggtggagcgg acagccgcga
    13261 ttttggaggc tcgcgaccgc agtctcgccg gcccgactgc tccggcccac ggactgacgc
    13321 tcgagtgggt gcgctacgat ccgccgcgga gctgccatga atcttgaggg tggcgtcgtt
    13381 ctggtgactg gcgcagcacg ccgcgtcggg cgggcaatcg ccctgcgttt cgctagcgcc
    13441 ggctgcggca tcgcaattca ctacctgcac tcgggcgaag aggctcaacg aaccgcagac
    13501 cagtgccggg cgctcggggt gaccgccgag acgttccgcg ccgacctgaa tgatcccgtg
    13561 gccaccgcgg ccctggtacc cgaggttttg gagcggttcg gtcgcctgga cgtactggtg
    13621 aacaatgctt ccgtgttcga gccgatgacg ctcgatacgt tttcgccggc ggaatgggag
    13681 cgtacgctcc gcataaatct gaccgccccg atgattttgg cgcacgcggc gcgggcggca
    13741 ttggaacaag ccggcgggcg catcatcaac ctggcggatg cggccgtggc gcacccgcgg
    13801 ccggaatatc tggcgtacgt ggtatcaaaa ggggcgctgg agacgttgac gcaggtgctg
    13861 gcgcgagcgt tggctccccg ggtgaacgtg gtcggcatcg cgcccggagt ggcagttttt
    13921 ccggagcatt atgatgccga aatgcggaaa cgcctgacga gccgaatacc gttgcagcgc
    13981 accggcacgg cagaagaagt ggccgagctg gccgagtttt tggtgcgagc gggcgatttc
    14041 atcaccggcg tggttatccc gatcgatggc ggtagacgac tcttgtaatg aatgcgcact
    14101 cgcacccgcc ggcaaagccg acaataatcg agttcgggcg gctgcgatcg tcgccggcct
    14161 cgtttacaat ggaggagtag gacccagagg tgtggaaatg tgctgttcgt ggaaactccc
    14221 agacctcgtc cgaaaaggcg ctccgcgccg agttcttctc tggtgcgccg caacgtttgt
    14281 cttcactccg atttgggcca gcgcccaggt tccgatcacg caggacgggc ggctcttcga
    14341 tgcaaacacg cagatctccg gctcgcgcta caatttcggc cggccgcagt cgcccctgct
    14401 gctgggcaac tcggtagcca ccgggaacgt gcgcagcggg ctgtctttcc ggggctttca
    14461 gcccatcagc gatccgacgt cgttcatgac cacgatcccg agcggcgcgc tctctaactt
    14521 tcgccgcgat tccgtgagtg tcgccgatgc ggcgctcgcg tctccgctga ttggcggacc
    14581 gtggtacgcg ccctcgctca cggcgccgac agttggattt ttgagcggtc aatttttgcc
    14641 gcgcacgccg atccctgatc agaggctcca accggcccgc gactcgggaa tcatgaccgc
    14701 cctgcgccaa ccgctcggcc cgattggtcc gccgccgtcg gccgtccgcg gcagcagcga
    14761 actgctctcg gcccagatta ttggcatgat tccaggggag ctgacgagtt cgatattcgg
    14821 gccgccacag ccgccggccg cgacacccac gcgcgagacg ttcacgccct ccgtcgttga
    14881 gccgggtctg aggggcggcc aggcgacaat cggacagacg ggcatcaact ggaccgcgcc
    14941 gtatctgcag gaagttccgg gctcgcgacc tccgctgggc acaatgctgg atctggttcg
    15001 agagcgtcgc ctgcagcgct tccccgtcga cacggagcag gaacccggca cgccgggcga
    15061 gctgctgccc cagccccagc cccagcccca gcttccctcc agcctcgggc cggtcatgcc
    15121 cgagcccgag caatcagacg catcgccctt aactttcggc ccgggagcac tggctgaacg
    15181 ctttggagaa agccgcacgc tacggccggg cgcgatggat tcggccggcg cggacctggc
    15241 cggcatttcg gctccgctgc cggacaccag cgtgttgccg ggctatgacg tcttcaccga
    15301 tatgcaactg gccatggctc tgagtggcaa cccgaacgca gactggttcg cccagatgca
    15361 gcgggccatc cgcgaaaacc cggccacggc tgtcatgctg cgcgagcggg ccgacgtgga
    15421 cgcccaggaa ttcatacagc agatgctcaa cagcccaatc cagaccttcc atgggcgcgg
    15481 cgagtcgccc aagaacaacg aaatgctgcg ggctgagtcg ctgatggaga tcgggcacta
    15541 ctttgaagct tcgcggcgtt accagatagc tcaccgtatc gatcccctcg atccgttgcc
    15601 gctgatcggc aggggcaatg cgctgctggc ggcgggcgag tacctgacgg ccgccttggc
    15661 gttgatgcag ggcttcgagc gttacccgga gctgtcgcgg tttgcgctgg atttgccgaa
    15721 gctggtgggc gggcgcgaga tcatcgatat ccgccgggcg gatatcatgc gcatgctcga
    15781 aacaagagac gatccgcagt tacgcttcct gttgggctat ctggagtact tctcgggcga
    15841 cgccaagctg cgcgaatccg ggctggcgaa tttcgaccgg gccgcacact tggacacgag
    15901 caattcgctg atttcgcgta tgccggccct gctgcggcac gagggcacgc ttccgccgcc
    15961 caagttgccc gaacaagatt ctttcctgcc ggaggagatt ccgaccccga cggagttagg
    16021 cgcaccagac cagaccagcc ccccgccgcc ggtcccgctg gacccgcccc agccccgtcc
    16081 tttggagaca ggcgagcccc aatgatacgt tttgttgtat tactgctacc cggcgttgtg
    16141 gcactctccg gatgcgctag tcagatggcc gccaccaacc agcagtcgcg ccttatacag
    16201 gagtgccaac ccgacaaagt gatgaccgta gcggccagga tgctgcagcg cgagttcggc
    16261 cgcgtgcgcg tgaattgcga ggctttaacc atcgaaaccg agccggccga gttcttgacc
    16321 acgtgtgaca gcggtacggc gcgcgaccta tatcggggaa cgtccacgat gcggcgcgtg
    16381 gcccatttca gcctcgcacg cagtgggtcc ggcacgctgg cccgcctgcg aattgacgtc
    16441 gagcggcgcg acaccgagcg ggtcgccaac gctcttatgc cgacgcagac cttgggcgat
    16501 ctgccgacgg tcagcgccac tcccatccag cgcgaagccg cgacgacgga gcgtcagaac
    16561 accgtctgga cctttgtacg ccgcgatcgg cagatggaac gatgtttgct ggacgagttg
    16621 gccagcgagt tcgcaccgcc ggctgtcgaa ccgcccgccc tggaggcgcc gggttcataa
    16681 ctgcacggct cagccgaact tacagacaac attcgcgccg aaaaggaaca aaccatgttg
    16741 aagtttatgt cccaaccgct tgcggaaact gaccccgaag tcgccggcat cattcggcgc
    16801 gaagaggaac gccaacagaa cacgctcgag ctgatcgcgt ccgaaaacca cgtttccgtg
    16861 gccgtgcgca ccgccgtggg ctccgtcttc accaataagt acgccgaggg ctatccgggc
    16921 aagcgctatt acggcggctg caccaacatg gattcggtcg aggacctggc ccgcgagcgc
    16981 gccaaaaagc tcttccacgc cgagcacgtc aacgtgcagc cgcactccgg cgcccaggca
    17041 aatacggccg tcttcatggc ctgtttgaag ccgggcgaca cggtgttgtc gctggacctg
    17101 gcgcacggcg ggcacctgac gcacgggctg aaggtgaatt tcagcggcac gatgtacaac
    17161 atcgtgccct acgtggtcag cccgaagacc gaaatgctgg acatggctga attgcggcgt
    17221 ctggcgctcg agcacaaacc caagctgatc atcgccggag cctcggccta tccgcgccgg
    17281 ctggatttcc cggctttcgc cgagatcgcc aaggaatgcg gagcgcggct catggcggac
    17341 atcgcccata tcgccggcct gattgcaacc gggctgcacc cgacgtcgat cggcttcgcc
    17401 gacttcacga ccacaaccac gcacaagacg ctgcgcgggc cgcgcggcgg aatgatcatg
    17461 tgcggcgcgg ccgacgcggc cctggtcgac aagtgggtct tcccgggcac ccaaggcggc
    17521 ccgctgatgc acgttatcgc cggtaaagcg acggcgtttg gcgaggctct gcggccggag
    17581 ttcaaggaat accagcagca gattctcagc aacgcgcagg ccctggcgga agcgctgcgg
    17641 gcgcgcggct accggctggt ttcgggcggg acggacacgc acctgatgct ggttgacctg
    17701 cgtgacgttc atccggacct gaccggcaag caggcgcagg actggctgga agaggcgggc
    17761 atcattacca acaagaacat gatcccatac gaccagcgca agcccacgca aaccagcggg
    17821 ctacggctgg ggacgccagc cttgaccacg cgcggcttga aggagccgca aatggctgtg
    17881 gttgcggagc tggtgcacca ggttctgctg gccaaaggcg accaggcggc gatgcagcgc
    17941 attcgtggcc aggtgcgtga cctctgcacg cagttcccgg tcccgcacgc gtaaggcaac
    18001 cgccaagacg caggccacgg caaccgccaa gacgcaaaga gcgctaagac cgccaagaag
    18061 agggttggca gcgtgtgcga gagactgcac gcgctgccgg cttcttattc ccccctgctg
    18121 ccttccgcct tcccccttct gccttctgtc ttctgacttc tggctccggc cttcttactg
    18181 atcgtaccgc atgtcgtaat agccggcttc gcggcgcgtg atgatttcag gcaggccgtc
    18241 gcgccgcaag atgaccggaa cctcaccgtc ttgcgtagcc tgcatcagcg gccttccctc
    18301 ctcgtctgtc gcgagccgcg gcaggaggtt gctccgatcg accgtgtatt gaacgcggat
    18361 gctggagtgc tgctgctccg gatcgcggtt catgagcgtc atgtagacgg tgaacgtatt
    18421 gctgcgcgtc gtgaggaaat gcgtatcgag cagcgccatc aggctgaccg ctttgaagaa
    18481 atcggggcaa ttcatttttc gcccgtcatt cggctgccca agcgggttgt acaggagatc
    18541 ggtgctgcgc ctcaaacccc tccaacccga gtgcgcatgg cgaaccgggc agtcaaaacc
    18601 cttgacattt agcaactcgc ccaaactcag gaagccgtgc ttcaggtcgc cacggcgaat
    18661 ggcgccgtac tggaagccgc caagcccgtc tccaggtatg gacgcgactt cgagtcggta
    18721 ttgcgcctca accgccgggc tcgtcgtccc atctgagtcg atcgaggcat tacgcaggtc
    18781 ggcggcccag agcgggttcc ggccggacga cagcgtaccc gggtcggacg cgtaacggag
    18841 ctggtcgcgg tacgagacga tcgcttgggc gtagtatggc ccgagcctga tccaataagg
    18901 ctccgtctga tccaccacaa gcagatcatt gggggactcg gctggtggtg ctgtcgccgg
    18961 tccgaagcgg tatagagccc caccggggag cccgacgact tctggagttt cgaactcgtc
    19021 cacaactcca gtgagaatac cgcagaccgg gctccagaac gcaggcgatg cggtatgatc
    19081 aatctggatt ttattcgggt cggttagatc ggggttgatc accggcaggc cggccaagac
    19141 gtaccagggt gcggtgttta tgtcgatgcg cccggggatt cggtgcgggt cgaggtccgg
    19201 tt
//
LOCUS       DAOSYS010000041        18967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2399_length_18967_cov_5.504019, whole genome shotgun sequence.
ACCESSION   DAOSYS010000041 DAOSYS010000000
VERSION     DAOSYS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18967
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2399_length_18967_cov_5.504019"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="PLU99_04075"
     CDS             complement(<1..1813)
                     /locus_tag="PLU99_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16266.1"
                     /translation="MRSSKYRVFATVAVTLLLGAAVGWSAGPHGFITNPAAPGIPTHG
                     PVVRGCGPETMTQLTSPTTVEASTSIACANESDGSTTDNSWARCFNVTQDITVNCVDF
                     GVESCDVEHVVDINIYRDNDGACPPNLATCQLLGSVSVAVPAGTALQILTADFNSIGG
                     VYVPAGSKMVVEVWSPDLTGQGAFFVGANNNGESADSYLRSEPCGVTDWMPIDAVWTS
                     PVHVVINVDYMFGTPNPGACCLENGDCLPDMLPTDCGAEGGSWQGSDTVCDPNPCEQP
                     PIGACCDLWGACQELDSYDCSQVGMWLGEGTVCTGDCDGDGAADVCEFELGTAEDCND
                     NGIPDNCDIADGTSCDANGNGIPDECEFRQRGDLNCDGASNVFDIDAFVLAMTDLTAY
                     YAQYPCCTHMNADCNFDGAVNVFDIDPFVVILTGGPAIIHPIELAGNPLAEYPHFEYV
                     RAFHVNAPISLAIDPSRHLFIIGRTADVYVVEKKSADQWAVDPTLIDVTAGGAMTVTF
                     SGGNIQGNTFQIVGPNELNASVYQAATGDYTGFGHGYDVVVDLNQNGVLDAGDCIDGL
                     GREAGLYVCHDSTARGPLAVTETLYNVGTIFGIPASVN"
     gene            complement(2289..3386)
                     /locus_tag="PLU99_04080"
     CDS             complement(2289..3386)
                     /locus_tag="PLU99_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOO16267.1"
                     /translation="MRIVLGKRGYRVIGRRKQRGAPVAVLLIVAGSMLACERLGCLGP
                     WRSTQPDRITSADSSGRAFFLDRPASADFRLMTYNVGWDSVFPSKDATRAERFQRIVR
                     AVAPDVLALQEIRQFDARETAALLDEISPRPAGERWHACRGKSGAVASRFPLIRAITR
                     LDPPTYRDPVIALIDLPDNRCAVDLCVVSNHFKCCGDANNDPIRQKQADGVMNWLRDA
                     RTPGGEVDIPEHTAFVFAGDLNLVGGPQPLLTLLTGDISDEALFGTDFAPDWDGTYLT
                     DAHPLHNGSGPDDYTWRKDDSGFDPSRLDYVLYSDSVLEAVNQFVLNTILMSEAELAN
                     SGLERFDCCHDSHGRELDHLPVVVDLRIRPR"
     gene            complement(4068..4349)
                     /locus_tag="PLU99_04085"
     CDS             complement(4068..4349)
                     /locus_tag="PLU99_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007097292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16268.1"
                     /translation="MPLLIIIFGLFAPRVVLVLAWCGGIFNGVWSTLLWPLLGLIFLP
                     YATLAYGLAYRYGSGLQGFWLALFILGIALDLGAIGGSARRRRRRALPD"
     gene            4600..4869
                     /gene="rpsO"
                     /locus_tag="PLU99_04090"
     CDS             4600..4869
                     /gene="rpsO"
                     /locus_tag="PLU99_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005528980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOO16269.1"
                     /translation="MPLTADKKAEISKEHRRHETDTGSAEVQIALLTSRISQLTEHLK
                     LHKKDHSSRRGLLKMVGKRSALLKFLARTDRERYLGTIAKLGLRK"
     gene            5069..7210
                     /gene="pnp"
                     /locus_tag="PLU99_04095"
     CDS             5069..7210
                     /gene="pnp"
                     /locus_tag="PLU99_04095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOO16270.1"
                     /translation="MMVKVERQIGANTLSIETGKLAKQAHGATLARYGDTVVLGTCVT
                     ADPRPGIDFFPLSVDYREKMSAAGKFPGGFFKREGRPTTKEVLTSRMIDRPMRPLFPE
                     GFRNEVQVQVMVLSADAENDPDVIGMVAAAAAVSISGVPFNGPVAAVRVGRIDRKFVI
                     NPSRAQLEYSDLDMVLAGHRQAVNMIEVGCRELSEEEVAEAVKFGHENGIVPICEMIE
                     ELQRQAGRPVTWEKPEPDAAFIEDIRRRVLDDLKAAKTITGKQERNEAVKAIYDRVIE
                     ELCPEGAENPEHTPDEVKAIISSIEEEYVRQRILKDHVRPDGRKLTEIRPISAEVGWL
                     PRVHGSALFSRGETQAMVTCTLGTSRDEQIVDDLVEEYSKKFLLHYNFPPFSVGEVRK
                     IMGPGRREIGHGALAERSLEAVLPSPDAFPYTIRIVSDILESNGSSSMATVCGGSLSL
                     MDAGVPISSAVAGISVGLVEGEKKHVLLADIIGEEDHFGDMDFKVAGTRKGITGIQLD
                     IKTQGLKHNIMVEALELARKCRLHILEQMDKALAEPRPEVSPNAPRLITIKINPEKIG
                     KLIGPGGKSIKAIQAATGAQIDIEDDGTVFISCTDAAKAKKAQEAVERITEDIQIGRI
                     YEGRVTSIKDFGAFIEIQDGQDGLCHISELADSYVKSVADVVQIGDHIKVKVIAIDEQ
                     GRVKLSRRAALREENGEEPEAVGPRRDKRKH"
     gene            7377..7610
                     /locus_tag="PLU99_04100"
     CDS             7377..7610
                     /locus_tag="PLU99_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOO16271.1"
                     /translation="MATGVVKWFDCRKGYGFIMGPGGEDVFVHYSALVCDGFKLLRQG
                     QEVEYDMHSTSKGLQALVVRPLERDRTIAALQV"
     gene            7632..9299
                     /locus_tag="PLU99_04105"
     CDS             7632..9299
                     /locus_tag="PLU99_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16272.1"
                     /translation="MATLIVLQGPDKGKTLNADDDVISIGRGAGSIPLGDQTVSRKHA
                     EMRRLNGDWVLMDLNSANGTYLNGMRLLQPVKIKRGDQIRVGSTLLVYTGDDRPAQLS
                     GASIPRDMVRLDAGSASVDASIVAIAPSNEDSIVLAAPETANAVKAWNVLRELSDVIG
                     SLLPPDRLLTRVMDIIFDQVDVERGVIFLRNRDGEVRPQVVRFRDGQTPANPDQKLVA
                     SRAIIDHVMNSREGVLSSNVVADKRFGGGRSMANDGLGSVVCAPIVARDQVLGAIHLE
                     CPVSQHTYNENELKLITAIGYQAGLAIDNARLVQSHLQQERLAAAGETVAYLSHYIKN
                     ILQGMRSGADVLQAGLDKQDLARAAQGWQIVERNLEKTYHLVMNMLAFSKQREPFLEM
                     YQINRIVEEVIALVQRQADDAHAILMTELDENLPAIPVDHEGIHQVVLNLLTNAIDAV
                     PPGKGAITVRTAFDAERREVIISVTDNGPGIAPEHRSKIFTPFHSTKGHGGTGLGLAV
                     ARKIVREMHGRLILVQPPDGGTEFRVTLPELQPSTRGVADTHGPGGT"
     gene            9514..10968
                     /locus_tag="PLU99_04110"
     CDS             9514..10968
                     /locus_tag="PLU99_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16273.1"
                     /translation="MAGDDKSEAQNEFSEADMAKARAWFTKAANCRERREYDYAIECY
                     ITGLGFWPEAVEEGHMPLRSLAIQRQQAGGAKAGVLEVLKKPLIGRNARQAMLNAEYL
                     LAKDPSSGSYLDSLLKNAFKAGMLETVRWVTPLVLDSLRKDKKPNKNRFKSFRDVVIA
                     AAEQADAKNQAPIAVWLLEHAVTSLEFQLAREPDEGLRNEQRDLAGRLAIVKGKYQEA
                     ESFRDSLRDAEQQKILHDSERVKQAEESYEALVAAARKALAENPDVPQKIYALVDVLT
                     RRESKESEGEAIALLLKSYEKSRNYNFKMRADQIRLGQLSRQTRQLFEQARRSGSEED
                     QQQYRLAKMEQSQVELEIYRERVQKYPTDLGLRYRLGRALFFAREFDEAIPALQAAQG
                     DPRHRYHARLLMSRAFFEKGAFAQAVDVLKEICDVPDLPEELGKDICYWLARSLEADG
                     QMDEARAAYSKLLRQDYNYADGEARRRLDALKSN"
     gene            10979..11254
                     /gene="rpsT"
                     /locus_tag="PLU99_04115"
     CDS             10979..11254
                     /gene="rpsT"
                     /locus_tag="PLU99_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOO16274.1"
                     /translation="MAHSLSSKKRIRQNETRRARNRARRSMLKTQLQACRAEIQRGTP
                     ETAQPVLLRAFKALDKAAVTGTIHRNAAARRKSRLAKRFNALRQQAS"
     gene            11817..12905
                     /locus_tag="PLU99_04120"
     CDS             11817..12905
                     /locus_tag="PLU99_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO16275.1"
                     /translation="MARSSIIKKLQLARAWAWRNPVWCAWQVNYRCNFRCSFCHYWHD
                     KLGDAPELPVEAFREGSRKLSQLGCLLVSLAGGEPLIRPDIVQIVEALAEWHFPFITT
                     NGWFATESLAEDLFAAGLWGVSVSIDYADAERHDRRRGTPGAFARALRAIEAFSRARV
                     HKFQRVNWMAVLMDDNLDQIEPMLRMAAERDAYFMLQPYGVRKTGSRRFICGAQGRVA
                     DYLLGLRRKYPNFLSNPYFLSKFDAALNGGVPGCKAGRGFFNIDSTGEIAVCVEERER
                     PVANLFSHPAGEIVRRLRARDMSQRCKACWYNCRGEVEALYRPDGLLLSLPTLLFDRG
                     RPPLGNSGANGQAGGVSGVQSCRPKVSS"
     gene            12902..13588
                     /gene="rsmG"
                     /locus_tag="PLU99_04125"
     CDS             12902..13588
                     /gene="rsmG"
                     /locus_tag="PLU99_04125"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOO16276.1"
                     /translation="MTLAELREAGYDLDAESYARLCRYVGLLLQANQKVNLISRASTD
                     QIWVAHICDSLALLPLIRERRPKSVLDLGAGGGLPGVPIACVHPELEVVLIDATRKKV
                     AAVEAIVSGIGQMHVRCIWGRAEALAREAELHERFDAVTARAVGTVAELLSYAADFLR
                     PGGQGWFFKTAPAAPVEVAAAAKQAHKAHLRHVENRAYRLPATGAARAIVVYEKSSAP
                     DGASGRAAIT"
     gene            13707..15182
                     /locus_tag="PLU99_04130"
     CDS             13707..15182
                     /locus_tag="PLU99_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16277.1"
                     /translation="MLVALSVAAASLCLPSLNLLAVTAAPADAPPTPTSAEDAGPATQ
                     PPAGTIPLIPYSDPTFGFQMQVPAGWVYDRTRFGGPQGSVGVLRGRNPNGQVALQILI
                     FRSDEITDFDTWLRTFVSRLSKNYGGKKIAEQRVTETESGDRSIPGERAILFVETGTA
                     SSLAHTYYLCIPFDPSTVWVLIMATPIGAPSDAEQCKVEFHRMADSVRVLYDPLEAAK
                     LEVAFQRGMEVLAKLRATAADVEIDENERFYDVLIGGKSIGYVSRWARRERRSIGDPR
                     YSPNLVPGLRVHEEVWRFADDGTVRQTVLDMFSSADLTKELIENHSTQIPAPDVPSQR
                     LFIQLDQCVREGHALVSSFSTNLDAELPEPRPPLPIGPRYLDLAWVRLLPRLLLDAPL
                     ETYAFAIYDSETHGLIICTIEPLGPVELPGPRGGRTYVFETREGFVAQPSRIYTDEHG
                     QIVRVESGDLAMVLTTADQVQHRYGALCEAARQRMAMPGQP"
     gene            complement(15202..16776)
                     /locus_tag="PLU99_04135"
     CDS             complement(15202..16776)
                     /locus_tag="PLU99_04135"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOO16278.1"
                     /translation="MAEMYLIAPLILLVVVTVAALLDRWSVPVILIALGAGILFGSDV
                     LAWWDFDDVALTNRMANLALVFILFQGGFSTRREDFRAVSLPAGGLATWGVILTAAVT
                     FLVLHLVFRWPLEKAMLLAVIISSTDAAATFSILRRQSLPPKLSSTVEIESAANDPMA
                     ILLTLAAIQAFTIGDARWYAVLLAFIWKFVAGVGIGWLLGHAAIWMFNRLRPQDRAHY
                     YILSFAVVLLIYGLGEVVRSSAMLAVFIAGYVMGNRPFVHKQGTANFVSALSTISETS
                     MFVMMGLLVFPHQWAHLWLQGILLFLVLTFVARPAAVWLGTLGMKLGAKNKLFISWAG
                     LRGAVPIILATYPIAAGMAVGQDVFNLVFFVVILSIAIQGSTLGVLARWLNLSTRSRP
                     KPFYNLELVTMAQSDMDLIVVDMPDPQGAPGPRISDLKLPAGSVITLITRGKDVVVPK
                     GSTRLQGWDQITVLAHAKDEPAVRAALLAGQDGQAEAADGKARRDPDDGRTGGVLEDR
                     DRPKLTTNNSELTTNN"
     gene            17219..18175
                     /gene="mdh"
                     /locus_tag="PLU99_04140"
     CDS             17219..18175
                     /gene="mdh"
                     /locus_tag="PLU99_04140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOO16279.1"
                     /translation="MKRAKITIIGAGNVGATCAMWATAAELADVVLVDIVEGMPQGKA
                     LDLAQAAPIINSDVRLVGSNGYEESADSEIVIITSGLPRKPGMSRDQLLETNVKIVAD
                     VTAQAVKHSPNAVLIVVSNPLDAMVYTAHKISGFPPHRVVGQAGVLDTARYHAFIAAE
                     IGCSVEDVQAMLLGGHGDDMVPLPRYTSVGGIPVTQLIPPGRLDEIVTRTRNGGAEIV
                     NLLKTGSAYYAPAAATIAMAESIIRDKKRVLPCAAYCKDEYEVGGYFVGVPCILGAGG
                     VEKVIEVELEPREREMFNASVAHVKELCEAAQKFLPKNKKAK"
     gene            18224..18949
                     /locus_tag="PLU99_04145"
     CDS             18224..18949
                     /locus_tag="PLU99_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16280.1"
                     /translation="MRLALVFLSISAVTAAFAQSKRADLPKEHRRAEQAAAAAAAVAA
                     AVEPIQCPVTGRPIDLNVSIEFEGRRVYFADATAREKFLDDPGQYAEAVRKQWDLMRP
                     PRVQVLCPVTGKPVDPEVSTLWNGVRIYFASAEAKATWEKDPQRYAAKLEESYTFQTV
                     CPCGYGDIRPDVSLEYNGRTLYFCCPGCREGFKRNPEAMLKQVDEQIAANKLKWERWR
                     SPQQRSGEQRGQGPATQDAPGGP"
BASE COUNT         3809 a         5950 c         5944 g         3264 t
ORIGIN      
        1 cgttcacact ggccggaata ccgaaaatcg tgcccacgtt gtagagcgtt tccgtgaccg
       61 ccaacggccc gcgcgccgtg gaatcatggc acacatacag gccggcctcg cgacccagtc
      121 cgtcaatgca gtcgccggcg tccagcacgc cgttctggtt caggtccacg accacgtcgt
      181 agccgtgccc aaagccggta tagtcgcctg tcgcggcctg gtacacggat gcattgagct
      241 cgttcgggcc cacgatctgg aacgtgttgc cctgaatgtt gccgccggag aacgtcacgg
      301 tcatcgcccc gcccgcagtg acatcaatca gcgtcgggtc gaccgcccac tggtctgcgg
      361 acttcttctc aaccacgtac acgtccgccg tgcgcccgat gatgaacaga tggcggctgg
      421 gatcgatcgc caggctgatc ggagcattca cgtggaaagc ccgcacgtac tcgaaatgcg
      481 ggtactccgc cagcgggttg ccggccaact cgatggggtg aatgatcgcc ggcccgccgg
      541 tcaggatcac cacaaacggg tcaatgtcga acacgttgac cgcgccgtca aagttgcagt
      601 cggcattcat gtgcgtgcag cagggatact gcgcgtaata ggcggtcagg tccgtcatcg
      661 ccagcacgaa cgcatcgatg tcgaacacat tcgacgcccc gtcacagttc aaatcgccac
      721 gctgccgaaa ttcgcactcg tccgggatgc cgttaccgtt cgcatcgcag ctagtgccgt
      781 cggcaatgtc gcaattatcg gggattccgt tgtcgttgca gtcctcggct gttcccagct
      841 cgaattcgca aacgtcagcg gccccatcgc cgtcgcagtc accggtgcaa acggtcccct
      901 cgcccaacca cattcccact tgggagcagt cgtacgagtc caattcctgg cacgcgcccc
      961 agaggtcgca gcaggcgccg atcgggggct gctcgcaagg attgggatca caaacggtgt
     1021 ccgagccctg ccacgatccg ccttcagcgc cgcaatcggt gggcagcata tccggaaggc
     1081 aatcgccgtt ctcgaggcag caggcgcccg gattcggcgt gccaaacatg taatcaacat
     1141 tgatcaccac gtgcaccggg ctcgtccaga ccgcgtcgat cggcatccag tccgtcacgc
     1201 cgcaaggttc agagcgcagg tagctgtcgg cgctctcgcc gttgttgttc gcgcccacga
     1261 agaacgcgcc ctgaccggtg aggtcaggcg accaaacctc gacaaccatc ttcgagcccg
     1321 ccggcacgta gacgccgccg atagagttga aatcggccgt cagaatctgc agggcggtcc
     1381 cggccgggac cgccacggac acggagccca gcaattggca cgtcgccaaa ttgggcgggc
     1441 aggcgccgtc gttgtcccga tagatgttga tgtcgaccac atgctcgacg tcgcagctct
     1501 caacgccgaa gtcgacgcag ttcaccgtga tatcctgcgt cacgttgaaa caccgcgccc
     1561 agctattgtc cgtcgtcgac ccgtccgatt cgttggcgca ggcgatcgac gtcgaagcct
     1621 ccaccgtggt gggcgacgtg agctgagtca tggtttccgg tccgcacccg cgcacaaccg
     1681 ggccatgcgt tggaatgccg ggcgcagccg gattcgtgat gaacccgtgc ggtccagccg
     1741 accagccgac cgctgctccc aggagcaacg ttacggctac cgttgcaaag acacgatact
     1801 tcgaacttct caaggaagac cctcctttgt taagttgaac aaacaaacta gcccaaacgg
     1861 gtacactccc ccctccacgc gccgcacagc gcgaagcaaa ccagcttggc acacaggaaa
     1921 ctgcggtaca ctatctcata taattaaccg aaaaccaccc cgcaaatcaa ctgcaatcca
     1981 acctttgttt tgtttgcgtc gcgacaaact cactatgtgc acgcatcggc gcgacgcctg
     2041 acccaggcca tgtgcgcacg ccttgacagc gccccgttgc cccggcgcca ctgaggcggt
     2101 tatccgggcc ggcgccctcg cgcaagctgt tgccgaacgg ctagttacgg ccaatcagca
     2161 ccacgtcccc taagcttcgc gccaaacagt ccagatccaa taagagatgc tcaaacggtt
     2221 tgccgaccga tgcccgaatc tccaatactc tcacacgtaa gctgctgaaa cacaacaact
     2281 tagacgcgtt accgcggcct gattcgcaga tcgaccacga ccggtaaatg gtccagctcg
     2341 cgaccgtgag agtcgtggca acaatcgaac cgctccaacc cgctgtttgc gagctcggcc
     2401 tcgctcatca aaattgtgtt cagaacgaac tgattcactg cctcgagcac gctatctgaa
     2461 tacagaacat agtcgagccg gctcggatca aagcccgaat cgtccttccg ccacgtgtaa
     2521 tcatccgggc cggaaccgtt gtgcaacgga tgtgcgtcgg tcaggtacgt accgtcccaa
     2581 tccggcgcga agtcagtccc aaagagcgct tcgtcagaga tgtcgccggt cagcagcgtc
     2641 agcagcggtt gcggcccgcc gaccaggttc aaatcgcccg caaacacgaa ggccgtgtgc
     2701 tcaggaatat ccacctcgcc gccgggcgtc cgtgcgtcgc gcagccagtt catcacgccg
     2761 tcggcctgct tttggcgtat gggatcgtta ttggcatcgc cgcagcattt gaaatgatta
     2821 cttaccacac acaggtcgac cgcgcaacgg ttatcgggca gatcgatcag cgcgatgacc
     2881 ggatcgcggt aggtcggcgg gtccagacga gtaatggccc ggatcagggg aaacctgctg
     2941 gcgacggcgc cggacttacc gcgacaagca tgccaccgct ccccagccgg tcgcgggctg
     3001 atttcgtcca gtaaggccgc agtctcgcgc gcgtcgaatt gccgaatctc ttgcagcgcc
     3061 aacacgtcgg gcgcgaccgc cctgacgatc cgttggaatc tctcggcccg cgtggcgtcc
     3121 ttactcggaa aaacgctgtc ccagccgacg ttgtaagtca tcagccggaa gtcggccgac
     3181 gccggccggt ccagaaagaa cgcccgcccg cttgaatcgg cggatgtgat ccggtcaggc
     3241 tgggtgcttc gccagggacc caggcaaccg aggcgctcac aggccagcat cgagccggcg
     3301 acgatcagca gcactgcgac cggcgcacca cgttgcttac gccggccgat gacccgatag
     3361 cctcgcttgc cgagcacgat gcgcacggcc gcgttctcca ccgcccgcgt gccgggagcg
     3421 cggcgccgac cgtcggcgtg cgcagcaaat tacccggcgc gcgtcaaact actcgcacgc
     3481 gcgccgggtg aaaagggttt cgcagcttgg cctattccat cgggccctta ctcgccgctt
     3541 cctgcttggc cttctctctc agcaagaacg tgcgggtgtc catattgggg tccaggatgt
     3601 acacctcgtt ggccggggcc tgagccggac tcggagcggc gcccggagtc gcgcccgggc
     3661 aggtgccagg gcacttcgga gtggaaggct gcgctgcttc ggttgttaca tgtgagctgg
     3721 agctcggtac gcttgggctg gcagtaaagg attcaggcga cgtcatctgc gccgaccggc
     3781 gcggctcaaa gagcctcttg aggaatcagc cgccgcccgg ggaacacgtt gcgcttcccg
     3841 aaacacagcc ccactgagag ccggcgaaaa cggccgcaat tccggcgatc accaaatatc
     3901 gcatgttgtt ctcaacgcta acagttatcg gcccgggcga attgtggggg catccggcgc
     3961 cgcttgcaag tagtccggca aatttgcggt ggttcaccgg gcgaacagtt gctcgcgcga
     4021 accactatta cgtgctaatc gattaggcga tcccgtatct cggatggtta gtctggcaga
     4081 gcgcggcggc gacgtctgcg tgccgagccg ccgatcgcgc cgaggtcgag agcgatcccg
     4141 agaatgaaca gcgccagcca gaagccctgc agcccggaac cgtaccgata cgcgagtccg
     4201 taggcgagcg tcgcgtaagg caagaagatc agccccagga gcggccagag cagcgtgctc
     4261 cagacgccgt tgaagatccc gccacaccac gccaacacga gaacgacgcg tggcgcgaac
     4321 agcccgaaaa tgatgatcaa cagcggcacg gccaagaccc aatcgcccgc tcgattatac
     4381 actctgccag tacgtggcct gagggccggc ccacgcacgg atgggtgacg ccgcgggcgg
     4441 cgcagcggcc ggcgtcacgt actcaaaaac gcttgcgact gggcgcgccg gttgcagctt
     4501 ccgaccaact gacatatagt acagggatta acaggcggtt ccggctgccc tgggcccgaa
     4561 ggtccggctg gagcctgccc tagcgtaaag gtaagggcca tgccattgac ggctgacaaa
     4621 aaagcagaga tcagcaagga acatcggcgc cacgagacgg ataccgggtc ggctgaggtg
     4681 cagatcgcgc tgctcacgtc gcgcatctct cagttgaccg agcacctgaa gctccacaag
     4741 aaggatcact cctcgcggcg cggtttgctc aagatggtcg gcaaacgctc ggcgctgctt
     4801 aaattcctgg ctcgcactga tcgcgagcgc tatctaggca caattgccaa gctcggatta
     4861 cgtaagtaaa tcccgcggtt ttgcctggca aatgccaggc cggccgggtg caaaccggtc
     4921 ccacaacaaa gggttaccgg tcccgcagcg gcggttgttc tacgctgcgc gcgccgagcg
     4981 ctgggggcgt tcagtctgtc atttcggctt gatcaaatcg tgttgttgga cggcagaggc
     5041 gaaagacggt tgttaaggtg tgtaggtgat gatggtaaag gttgaaagac aaatcggtgc
     5101 taacacgttg tcgatcgaaa cggggaaact cgccaagcag gcgcacggcg ccactctggc
     5161 tcgctacggg gacacggtcg tactcggaac ctgcgtgacg gccgacccac gcccgggaat
     5221 cgatttcttc cccctctcgg tcgactaccg tgagaaaatg tccgccgccg gcaagtttcc
     5281 gggcggtttc ttcaaacgcg aggggcgccc cacgaccaaa gaggttttaa cgagccgcat
     5341 gatcgatcgc ccgatgcggc cgctgtttcc ggaaggtttt cgtaacgagg tacaggtcca
     5401 ggtgatggtg ctctcggccg acgccgagaa cgacccggac gtcatcggca tggttgcggc
     5461 ggcggcggcc gtttccattt cgggcgtgcc gttcaacggc cccgtggccg cggttcgcgt
     5521 cggccggatc gaccgcaagt tcgtgattaa tccgtcgcgc gcccaattgg aatactctga
     5581 cctggacatg gtgctggcag gccatcgtca agcggtgaac atgatcgagg tcggttgccg
     5641 cgagctgtcg gaggaagagg tagctgaggc ggtcaagttc ggccacgaga acggtatcgt
     5701 gccgatctgt gagatgatcg aagagctcca gcggcaggcc ggccggccgg ttacgtggga
     5761 aaagcctgag cccgacgcgg ccttcatcga ggatattcgc cgccgcgtgc tggacgacct
     5821 caaggctgcc aagactatca cgggcaagca ggaacgaaac gaagcggtca aagcgattta
     5881 tgaccgcgtg atagaggagc tgtgccccga aggagctgag aacccggagc acaccccgga
     5941 tgaggtgaag gccatcatct cctccatcga ggaggagtac gtccgccagc gcattctgaa
     6001 agaccacgtc cgccccgatg gacgtaagct gacagagatt cgccccatca gtgcggaagt
     6061 gggctggctg ccgcgcgtcc acggctcggc gttgttcagc cgcggggaaa cgcaagccat
     6121 ggtcacgtgc acactgggga cgtcgcggga cgagcagatc gttgacgacc tggtggagga
     6181 atacagcaag aagttcctgc tgcactacaa ttttccgccg ttctcggtgg gcgaggttcg
     6241 caagattatg ggacctgggc ggcgcgaaat cggccacggc gcgttagccg agcgctccct
     6301 cgaggcggtc ctgccctcgc cggacgcctt cccgtacacc attcgcatcg tcagcgacat
     6361 tttggagtcg aatggttcaa gttccatggc gaccgtctgt ggcggatcgc tcagtctgat
     6421 ggacgccgga gttcccataa gctcggccgt tgccgggatc tcggtcggcc tggttgaggg
     6481 tgagaagaaa cacgtgctgc tggccgacat cattggcgaa gaagaccact tcggcgacat
     6541 ggatttcaag gtggcgggga cgcgtaaagg cattaccggc atacagttag acataaagac
     6601 ccagggcttg aagcacaaca tcatggttga ggcccttgaa ctcgcccgga aatgtcggct
     6661 tcacattctc gagcagatgg acaaagccct cgccgaaccg cgcccggagg tcagcccgaa
     6721 cgcgccacgc ctgatcacga tcaagatcaa tcccgagaag atcggaaaac tgatcggacc
     6781 gggcggcaaa tccatcaagg ccatccaggc cgccaccggc gcccagatcg acatcgaaga
     6841 cgacgggacc gttttcatct cctgcaccga cgccgcaaag gccaagaaag cccaggaggc
     6901 ggtggagcgg atcacggaag acatccagat cggtcggatt tacgagggac gggttaccag
     6961 cattaaggac ttcggagcgt tcatcgaaat ccaggacggc caggacggcc tgtgccatat
     7021 cagcgaattg gcagattctt acgtgaaatc tgttgcagat gtagtgcaga ttggtgatca
     7081 tataaaagtc aaagtgatcg cgatcgacga gcagggccgt gtcaagctct cgcggcgcgc
     7141 cgcgctgcgt gaggagaacg gtgaggagcc cgaggctgtc gggccgcgcc gcgacaaacg
     7201 caagcactag ttgtctcccg cgacgccggt caattcaatg cgtctaaacc ggcgtttggt
     7261 ggaagcgtat aaagaaatgc ccggttccac tcccgcggcg catggccgcg ggagcggcac
     7321 gttccagttg gccgtccgcg caagaggcac atctgcgcgg cttacggagg tcggccatgg
     7381 cgaccggcgt cgtgaagtgg tttgattgca ggaaaggcta cggttttatt atgggcccgg
     7441 ggggagaaga tgtgttcgtg cactacagcg cgctcgtttg cgacgggttc aaactgttgc
     7501 gccaggggca ggaagtggaa tacgacatgc atagcacttc caaaggcctc caggcgctag
     7561 tggtccggcc gctggagcgc gataggacga tcgccgcttt gcaggtttaa cggcgatcct
     7621 ggtggtgtgc tttggccacg ctgattgttc tacaaggtcc cgataagggc aaaacgctca
     7681 acgccgacga cgatgtgatc tccatcgggc gcggcgccgg cagcattccc ttgggcgatc
     7741 agaccgtcag ccgtaagcac gccgagatgc ggcgcttgaa cggcgactgg gtgctgatgg
     7801 acctgaacag cgccaacggc acctacctga acggcatgcg cctgctgcag ccggtcaaga
     7861 ttaagcgcgg cgaccagatt cgggtgggca gcaccttgct ggtctacacg ggtgacgacc
     7921 gccccgctca actctccggc gccagcattc cccgcgacat ggtcaggctc gacgcgggaa
     7981 gcgcctcagt cgacgcaagc atcgtcgcca tcgcaccttc caacgaagac agcatcgtgt
     8041 tggccgcgcc ggaaacggct aacgcggtca aagcctggaa tgtcctgcgt gagctgtcag
     8101 acgtcatcgg cagcttgttg ccgccggatc gcctgctgac gcgcgtcatg gacatcattt
     8161 tcgaccaggt ggacgtggag cgcggggtga tcttcctgcg taaccgcgac ggggaggtca
     8221 ggccgcaggt ggtccgcttc cgggacggcc agacgccggc caatccggac cagaagctgg
     8281 tggcctcgcg tgcgatcatt gaccacgtga tgaattcgcg ggagggcgtg ctctcgtcga
     8341 acgtggtagc tgataagcgg tttggcggcg ggcggagcat ggccaacgac gggctgggtt
     8401 cggtcgtctg tgcgccgatc gtggcccgcg accaagtgtt gggcgcgatc cacctggaat
     8461 gcccggttag ccagcacacg tacaacgaaa acgagctgaa gctgatcacg gcgattggct
     8521 accaggccgg gctggcgatc gacaacgccc gcctggttca atcgcacttg cagcaggaac
     8581 gcctggcggc ggcgggcgag accgtcgcct atctctccca ctacatcaag aacattttgc
     8641 agggcatgcg gagcggggcc gacgtgctgc aggccggcct cgacaagcag gatctggcgc
     8701 gggccgctca gggctggcag atcgtcgagc gcaatctcga gaagacctac cacctggtga
     8761 tgaacatgct cgccttcagc aagcagcgcg agccgttcct cgagatgtac cagattaatc
     8821 gcatcgtcga agaggttatc gccttggtgc agaggcaggc cgacgatgca cacgcgatac
     8881 tgatgaccga actcgacgag aatctgccgg ccatcccggt ggaccacgag gggattcacc
     8941 aggtcgtgct caacctgttg accaacgcca tcgatgccgt gccgcccggc aagggcgcga
     9001 tcaccgtgcg caccgctttc gacgccgagc gccgcgaggt catcatttcg gtcaccgata
     9061 atgggcccgg aatcgcgccc gaacatcgct ccaaaatctt cacccccttc cactccacca
     9121 aaggtcatgg cggtacaggc ctgggactgg cagtggctcg caagatcgtg cgggaaatgc
     9181 acggccggct gatattggtg cagccgcccg acggcggcac cgaattccgg gtcacattgc
     9241 cggagttgca accgtccacg cgcggcgtcg ccgacacgca cgggccgggc gggacgtagg
     9301 ccaccggccg gcctgcctca ccacgatttg gctgcacggc gccgccgcgg ccgaaacccg
     9361 ccatgaacca tcgccaagcc gtcccgccac cggctcagct cgcgctgcga catagccctt
     9421 tccggccttt ccgcatgccc ggattcacgc tatactaccc ggttcccggt agactggcgg
     9481 ctggttgaat cgcctggcgg caagcaggaa acgatggcgg gagacgacaa gagcgaggcc
     9541 cagaacgaat tctccgaagc cgacatggct aaagcgcgcg cctggttcac caaggccgcc
     9601 aactgccgtg agcgccggga gtacgactac gccatcgagt gctacatcac cggtctaggc
     9661 ttctggcccg aggcggtcga ggaagggcac atgcccctgc gctcgctggc cattcagcgg
     9721 cagcaggccg gcggtgccaa ggcgggcgtc ttggaggttc tgaagaagcc gctgatcggc
     9781 cgaaacgcac ggcaggcgat gctcaatgcc gagtacctgc tggcgaagga ccccagcagc
     9841 ggcagctatc tggacagctt gcttaagaac gcgttcaaag cgggaatgct cgaaaccgtg
     9901 cgctgggtta cgccgctggt cctggactcg cttcgcaaag acaagaagcc gaacaagaat
     9961 cgcttcaagt ccttccgcga cgtggtcatt gcggcggccg agcaggccga cgcgaagaac
    10021 caggccccca tcgcggtctg gctgctggaa cacgcggtaa catccttgga attccagctc
    10081 gcccgcgaac ccgatgaggg actgcggaac gagcagcgcg atctggccgg ccgcttggcg
    10141 atcgtcaagg gcaagtacca ggaagctgaa agcttccgcg attcgctgcg cgacgccgag
    10201 cagcagaaga ttctgcacga cagcgaacgc gtcaagcagg ccgaggaatc gtacgaagcc
    10261 ctggtggctg cggcccgcaa ggcactggcg gaaaacccgg atgtgccgca gaagatttat
    10321 gctttggtcg acgtcctgac gcggcgtgag agtaaggaaa gcgaaggcga ggcgattgcc
    10381 ctgctgctca agtcgtacga aaagtcccgt aattacaatt tcaagatgcg ggcggatcaa
    10441 attcgcttag ggcagctctc gcgccagacg cgccaattgt tcgagcaggc gcggcgctcc
    10501 ggatccgagg aggatcagca gcagtaccgc ctggcgaaaa tggagcagtc gcaggttgaa
    10561 ctcgaaatct accgcgagcg cgttcagaaa tacccgaccg acttgggcct gagataccgc
    10621 ctgggccggg ctctgttctt tgcgcgcgaa tttgacgaag ctattccagc gctgcaagct
    10681 gcccagggcg acccgcgaca ccgctatcac gcccgcctgt tgatgagccg cgcttttttc
    10741 gagaaaggcg cgttcgctca ggccgtcgac gtgctcaaag agatttgcga cgtccccgac
    10801 ttgccggaag agctggggaa ggatatttgc tactggctgg cgcgctcact tgaggccgac
    10861 ggccagatgg acgaagcccg tgcggcttat agcaagctgc tgcggcagga ttacaattac
    10921 gccgacggcg aagcccgccg gcggctggat gcattgaagt cgaactgagg aatcccaagt
    10981 ggcccattca ctatcgtcaa agaaacgcat caggcagaac gaaacccgcc gcgcgcgcaa
    11041 ccgcgcccgc cgcagcatgc tcaaaacgca gttgcaggcc tgccgcgccg aaatacagcg
    11101 cggcacgccc gaaacggccc aacccgtcct cctgcgcgca tttaaagccc tcgacaaggc
    11161 tgctgtgacc ggcactattc accgcaacgc ggccgcccgg cgtaagagcc ggctggcgaa
    11221 gcgcttcaac gccctgcggc aacaggccag ctaaaaaccg gtcaataaga ccgcgcttgg
    11281 cagtgcgccc ggtgccgttc aatccgagcc cgaagcgcta gcgagggcca aatgcccgcc
    11341 gaattcaccg gctaaacaaa caccggcagc cgcttcctga agcgactgcc ggtgcgagag
    11401 taaggagggt agtcagcgat actaccatcg acgcattcgg cccggcgact tgacgtccac
    11461 ggcgtgccaa accctctgtc gacaacggtt tgcgcgctgc aagccaagaa accaatcgcg
    11521 gcaaagccgc aacacgtcct ataaaagacg gccaggcagc agcacgaccc gttatcttca
    11581 cccaaactgc tctgaaaata tctgctagcc gcaggatcgc ctaaagactc catctgctgc
    11641 cagcaagagc gtctgcttaa cgtcttcaaa atcgatttct gttgaattga acgcgatcat
    11701 gtcgggaact gggcatcagc gagatggcac cggcttcctc ccccccccct tgttcgaggg
    11761 caacttacgt atatattcga aagcacggcg gggcgcggct tggcgatgtg ggagaagtgg
    11821 cgcggtcttc gatcataaag aagttgcagc ttgcgcgagc ctgggcttgg cgcaaccccg
    11881 tctggtgcgc ctggcaggtc aactatcgct gtaatttccg ttgcagtttc tgccattatt
    11941 ggcacgacaa gctgggcgac gctcccgagc tgcccgtcga agctttccgg gagggatcgc
    12001 gaaagctgtc ccaactgggc tgcctgttgg tgagcctggc cggcggagag ccgttgattc
    12061 gcccggatat tgtgcagatc gtcgaagcgc tggctgaatg gcatttcccc ttcatcacca
    12121 ccaatggctg gttcgcgact gaaagcttgg ctgaagacct cttcgccgcc gggctgtggg
    12181 gcgtttcggt cagcatcgat tacgccgacg ccgagcggca cgaccggcgg cgtggcacgc
    12241 ccggcgcgtt cgcgcgcgcc ctgcgagcca tcgaagcctt cagccgggcg cgagtccaca
    12301 agtttcagcg cgtgaactgg atggccgtgc tcatggatga caacctcgat cagatcgagc
    12361 ccatgcttcg catggccgct gagcgggacg cgtacttcat gctgcaacca tacggcgtgc
    12421 ggaaaaccgg ctcgcgccgt ttcatctgcg gcgctcaggg ccgggtggct gactacctgc
    12481 tcggcttgcg ccgcaagtac cccaatttct tgagcaaccc gtacttcctc tcgaagttcg
    12541 acgcggctct taacggcggc gtgccgggct gtaaggcggg tcgcgggttc tttaatatcg
    12601 attccaccgg cgagatcgcg gtttgcgtgg aagagcgcga gcggccggtg gccaatctgt
    12661 tttcgcatcc ggccggcgag attgtgcggc gtttgcgggc ccgggacatg tcgcagcgct
    12721 gcaaggcgtg ctggtacaac tgccgcgggg aggtcgaggc cctttaccgg ccggacgggc
    12781 tgctgctcag tctgccgacg ctgctttttg accgggggcg tccgccgctg gggaattccg
    12841 gcgcgaacgg ccaggccggc ggggtgagcg gcgtgcaaag ctgccggccg aaagtcagtt
    12901 catgacactg gcggagctgc gcgaagcggg gtacgacctc gacgccgagt cctatgcacg
    12961 cctctgccgc tacgttggct tgctcttgca ggccaaccag aaggtgaacc tcatatcgcg
    13021 cgccagcacc gatcaaatct gggtcgcgca tatctgcgac agcctggcgc tcttgccgct
    13081 aattcgcgag cgacggccga aaagcgtgct cgacctcggc gccggcggcg gcttgccagg
    13141 tgtgccgatc gcctgcgttc acccggagct ggaagtcgtg ctgattgacg ccacgcgcaa
    13201 gaaagtagcg gcggtggagg ccatcgttag cggcatcggc cagatgcacg tccggtgcat
    13261 ctgggggcgg gccgaggctc tggcgcgtga ggccgagctg cacgaacggt ttgatgcggt
    13321 taccgcccgg gccgtcggca cggtcgcgga gctgctgagc tacgcggccg attttcttcg
    13381 gcccggcgga caaggttggt tcttcaaaac cgcgccagcg gcgccggtcg aagtcgccgc
    13441 tgctgcgaaa caggcccaca aggctcatct gcggcacgtt gaaaaccggg cttatcgatt
    13501 gccggctacg ggcgccgcgc gcgcgattgt cgtttacgag aagagcagtg cgccagacgg
    13561 cgcctcagga cgggccgcca tcacctaagc aacgggacac gctccgtcat ccgtgggccc
    13621 ggacaagttt gatcggatcg cgtaaactgc gcgggacgag tcgggccggc tttcggccct
    13681 accagcccgg attttcgagg ctccacatgc ttgttgctct ttctgtcgcc gccgcctcgc
    13741 tgtgcctgcc ttctctgaac ctgctcgctg tcactgccgc cccggcggac gcgccaccaa
    13801 cgcccacttc cgccgaagac gccggccccg ccactcagcc gcctgccggc acaatcccgc
    13861 taatacccta ttccgacccc acctttggct tccagatgca ggttcccgcc ggctgggtct
    13921 atgaccgcac gcgcttcggc ggtccccaag gttccgtcgg cgtgctccgc ggccgtaacc
    13981 ccaacgggca ggttgccctg caaatcctca tcttccgcag cgatgaaatc acggatttcg
    14041 acacctggct gcgaacgttt gtcagccggc taagcaagaa ctatggcgga aagaagatcg
    14101 ccgagcagcg cgtcaccgaa accgaaagcg gagaccgctc cattcctggc gagcgggcca
    14161 ttctgtttgt tgaaaccggc acggcctcca gcctggcgca tacttactac ctctgcatcc
    14221 cgttcgaccc cagcacggtc tgggtgctaa tcatggccac gccgatcggc gcaccgtccg
    14281 acgcggaaca gtgcaaggtc gaattccacc gcatggccga ttccgtccgc gtgctctacg
    14341 acccgctcga agccgccaaa ctggaggtgg ctttccagcg cgggatggaa gtgctcgcga
    14401 aactgcgggc cacggcggca gacgtggaga tcgacgaaaa cgagcggttc tatgacgtgc
    14461 tgatcggcgg caagtcgatc gggtatgtct cgcgctgggc gcggcgcgag cggcgcagca
    14521 tcggtgaccc gcgctactcg cccaatctcg tgcccgggct gcgcgtgcac gaggaagtct
    14581 ggcgctttgc ggacgacggc accgtgcggc agaccgtgct cgacatgttc agcagtgccg
    14641 acctgacgaa ggagctgatc gagaatcact ctacgcagat tcccgcgccg gacgtgccct
    14701 cgcagcgcct gttcatccag ctcgaccagt gcgtgcgcga agggcacgcc ctcgtgtcga
    14761 gcttcagcac caacctcgat gccgaactgc ccgagccgcg cccgccgctg cccatcggac
    14821 cgcgttacct cgacctggcc tgggtgcgtc tgctgccgcg cttgctgttg gacgcgccgc
    14881 ttgaaaccta cgcgttcgcc atctatgact cggagacgca cggcctgatc atctgcacca
    14941 ttgagccact gggcccggtc gagcttcccg ggccgcgcgg cggacgaacc tatgtcttcg
    15001 aaacgcgcga aggcttcgtc gcccaaccca gccgcattta caccgacgaa cacggccaga
    15061 ttgtgcgcgt tgagagcggc gatctggcga tggtgctcac cacggccgac caagtacaac
    15121 accgctacgg cgccctgtgc gaagccgcgc ggcaacggat ggctatgccc ggccagccgt
    15181 gagcgggccg cgcgtcagcc gttagttgtt agtcgttagt tctgagttgt tagttgttag
    15241 ttttggccgg tcacggtctt caagtacgcc gccagttcgt ccatcgtcag gatctcgtcg
    15301 cgctttgcca tcggcggctt ctgcctggcc gtcttgcccg gccaatagcg cggcgcgaac
    15361 cgctggttca tccttggcat gcgccaggac ggtaatctgg tcccagcctt gcaggcgcgt
    15421 actgcccttg ggaacaacta catccttgcc gcgcgtgatc aacgtgatga cagacccggc
    15481 cggcagcttc aagtcggaga tccgaggtcc tggcgcacct tggggatcgg gcatgtccac
    15541 gacgatcagg tccatgtcgc tttgcgccat cgtgaccagt tcaaggttgt agaacggctt
    15601 tggacgagaa cgcgtggaaa gattgagcca tcgggccagc acgcccaggg ttgagccctg
    15661 aatcgcgatg gacaggatca ccacgaagaa caccagattg aaaacatcct ggccgaccgc
    15721 cattcccgca gcgatcggat aagtcgccaa gatgatcggc accgcgccac gcaggcccgc
    15781 ccacgaaata aacagcttgt tcttcgcacc caacttcatt cccagcgtgc cgagccaaac
    15841 ggccgccggc cgggcgacga acgtcaacac gaggaagagc aggatgcctt gcagccacaa
    15901 atgcgcccac tgatgcggga aaaccagcaa gcccatcatg acgaacatgc tggtttcaga
    15961 gatcgtggag agcgccgata caaagttggc cgtgccttgc ttatgaacaa agggccggtt
    16021 gcccattaca tagccggcga tgaagaccgc cagcatcgcg ctgctcctga ccacctctcc
    16081 caagccgtag atgagcagca ccacggcgaa cgacagaatg tagtagtggg cacgatcctg
    16141 ggggcggaga cgattgaaca tccagattgc cgcgtggccg agcaaccagc cgatgccaac
    16201 gcccgcgacg aatttccaga tgaacgccaa aagcacagcg taccagcggg catctccaat
    16261 ggtgaaggcc tggatggcgg ccagggtcaa caggattgcc atcgggtcgt tggccgcgct
    16321 ttcgatctcc acggtcgaag agagctttgg gggcagcgac tggcggcgga gaatcgagaa
    16381 cgtggcggcc gcgtccgtgg acgagatgat gaccgccagc agcattgcct tttccagcgg
    16441 ccaccgaaac accagatgaa gcaccaggaa agtgacggcg gccgtgagga tgacgcccca
    16501 ggttgccagt ccgccggccg gcaaggacac cgctcgaaag tcttcgcgcc gggtgctgaa
    16561 gccgccctga aagaggatga agaccagggc cagattggcc atccggttgg tcagggccac
    16621 atcgtcgaag tcccaccagg cgagtacatc gctgccgaac aggattccag cgccaagggc
    16681 gatcagaatg accggaacac tccagcgatc cagcagggcc gccaccgtga cgaccacgag
    16741 gaggatcaag ggggcgatca ggtacatttc agccatcgag cgggttttcc ctccaggcag
    16801 gtgaatctgg ccagccggca agcgcggcgg ttaccggaaa agcccggcca tccgcacccg
    16861 tcgttcgtcg cttgcgaagg ctcacgccga ccattatccg tatttcgccg gcgttcacaa
    16921 ccggaaaccg ctgagctcgg cgtatctgcc ggacgccgag gagagcagag ccggggcgtg
    16981 ccgccccgaa atgccgctaa gaacctcgct ttcgcgcagc gcgcagtacc gcgtttcaca
    17041 ctccgcgcga gctttgcgcc ctgggcgctt ccacccccga ccgcgccaca caccggcctc
    17101 ctgtaagcac aatttgcact agccctccat tgcgcctgct gttggcccga cggccagcag
    17161 gacgataaaa tcccactgga cgcggtgcaa gggaagcaac cattcatgga gccgtaaaat
    17221 gaaacgcgcg aagatcacga ttatcggagc cggcaacgtg ggcgcaacct gcgcgatgtg
    17281 ggctaccgcg gccgagctgg ctgatgtcgt gctcgtggac attgtcgagg gcatgccgca
    17341 aggcaaggcc ctcgatctgg cccaagcggc gccgattatt aattctgacg tgcgcctggt
    17401 cgggagtaac ggctacgaag agagcgccga cagcgagatc gtgattatca cgtccggcct
    17461 gccgcgcaag ccggggatgt cgcgcgacca gttgctggaa acgaacgtca agatcgtcgc
    17521 ggacgtcacc gctcaggccg tgaagcacag cccgaacgcg gtgctgatcg tcgtctccaa
    17581 tccgctggat gcgatggtgt ataccgcgca caaaatctcc ggcttcccgc cgcaccgcgt
    17641 cgtgggacag gccggcgtgc tggacacggc gcgttaccat gcgttcatcg ccgctgaaat
    17701 cggatgcagc gtcgaggacg tacaggccat gttgctgggc gggcacggcg acgacatggt
    17761 tccgctgccg cgctacacgt cggtcggcgg aatcccggtg acgcagctca ttccgccggg
    17821 gcggctggac gagatcgtca cgcgcacccg caacggcggg gccgaaatcg tgaacctgct
    17881 gaagaccggc agcgcgtatt acgccccggc cgcggcgacc atcgctatgg ccgaatccat
    17941 aatccgcgac aagaaacgcg tcctgccgtg cgccgcgtac tgcaaggacg agtacgaggt
    18001 gggcggatac tttgtggggg tgccgtgcat tctgggagcg ggcggggtag agaaggtgat
    18061 cgaggtcgag ctcgagccgc gcgagcgcga gatgtttaac gcgagcgtcg cacacgttaa
    18121 ggagttgtgc gaggcggccc agaagttcct gcccaagaac aagaaggcga agtaagcgcc
    18181 tcgccggcgg caggccgagt tacaatcaac cggaggcctg attatgcgcc ttgcgctagt
    18241 ttttctaagc atttcggccg tgacggcggc gttcgcgcag agcaagcggg cggatttgcc
    18301 caaagagcac cgccgggccg agcaggctgc ggcggccgcg gcggctgtgg cggcggcggt
    18361 cgagccgatc cagtgccccg tgacggggcg gcccatcgac ctcaacgtct cgattgaatt
    18421 tgagggcagg cgggtttatt tcgccgacgc gaccgcgcgg gagaagtttt tggacgaccc
    18481 cgggcagtac gcggaggcgg tgcggaagca gtgggattta atgcgcccgc cgcgcgtgca
    18541 ggtgctttgc ccggtgaccg gcaagccggt ggaccccgag gtttcgacgc tgtggaacgg
    18601 cgtgcgcatt tacttcgctt ccgcggaggc gaaggcgacc tgggagaagg acccgcagcg
    18661 ctacgccgcc aaacttgagg agtcgtacac gttccagacc gtctgcccgt gcggctacgg
    18721 cgacatccgg ccggatgtga gcctggagta caacggacgg acgttgtatt tctgctgccc
    18781 cggctgtcgc gaggggttca agcgcaatcc ggaagcgatg ctgaagcagg ttgacgaaca
    18841 aatcgcggcc aacaagctga aatgggagcg gtggcgctcg ccgcagcagc ggtcgggcga
    18901 gcagcgggga caagggccag cgactcagga cgcgcccggc gggccgtgaa agcgcgcgtc
    18961 ggtgatc
//
LOCUS       DAOSYS010000042        18477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2493_length_18477_cov_5.786994, whole genome shotgun sequence.
ACCESSION   DAOSYS010000042 DAOSYS010000000
VERSION     DAOSYS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2493_length_18477_cov_5.786994"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLU99_04150"
     CDS             <1..596
                     /locus_tag="PLU99_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16281.1"
                     /translation="GRCYVTDSRRTYDVDGGPTHLTSPAFDMSAMHSPIVRFAEFFFC
                     DDATVYPPTQDFLDVHLSSDGGATWVLAAQYASHDDWAMHEIRVADFIPLTATVQVRF
                     TANDTPNNSQTEAGIDAVWIYDLACDAPWKLGDVNCDQAVNVFDIDPFVLALTDQAEY
                     VATYPDCNVMNADTNGDGAINVFDIDPFVNLLTGAQP"
     gene            complement(789..1730)
                     /locus_tag="PLU99_04155"
     CDS             complement(789..1730)
                     /locus_tag="PLU99_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOO16282.1"
                     /translation="MPAMVFGVIVVVVVLVLLSGFFTVQQQTHAVVQRFGRFLRICGP
                     GLHFRWPLVDTVAGRITHRVRELEIKVETKTKDDVFVELLIAVQFFVRETEEAVKSAQ
                     YKLMNPQQQISSYVFDTVRALVPEMPVDNVFSEKEKIASAVKERLEQIMDDFGYTILQ
                     ALVNDIQPDPEVKAAMNKVNASARLKEAAKNEAEARKIRVIAEAEAEARAKELQGVGI
                     ARQRLAIAKGLKESVESCSEAGISPEEATKMVLLTQHYDSVTAVGSRSGATIMMVPYS
                     PAGMSAFSDQIMQALLGAKQAQETHTGSVAPDGSRST"
     gene            complement(1758..2219)
                     /locus_tag="PLU99_04160"
     CDS             complement(1758..2219)
                     /locus_tag="PLU99_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16283.1"
                     /translation="MELACPRCRIANPEVARFCRRCGLALVRGPDGSLLGPGQIRHPQ
                     PLAAPPGFQCFDDTPDLFFSMKSAWGGQALSDTEPRIIVAFNAGYSLVDVIILIRGHG
                     PAGEAAFCIPHAVERWGRGQTVTLEVPSWELPDEPHELTLALESAEFDAEN"
     gene            complement(2316..3437)
                     /locus_tag="PLU99_04165"
     CDS             complement(2316..3437)
                     /locus_tag="PLU99_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16284.1"
                     /translation="MPWEGEAPVEPRRARRFALWAPVLAALVLSLAGCQSPQQQANQE
                     ATQRWNQARAQLKAKLAYDQFAAGNVKAAAGELAEARRLDPANMNWTPLQARIWLAEG
                     NTAAAADLLDRTQLDGPVQAEVEYLRGIVYQQQQRWEDAVAAFERAAELDQSEVGYVV
                     AAVQVWLQLGQPRQALQCLEACATNFAWTNAYQAALAEALELAGDWSGAAAAWRRVLQ
                     AADDDAGISERLAEALCRAGRYGEAIPVLQQLLNAEQAQQSTGVRLRLAEALLAVNQP
                     EAARVQAQAVLQSDPNQVSALLLLAQAWAAAGDTGAALRTAERALQLQPQDARVQELT
                     AALEWSAGQESRARERARRLREIDPANPVALHILSLGSY"
     gene            complement(3457..5322)
                     /locus_tag="PLU99_04170"
     CDS             complement(3457..5322)
                     /locus_tag="PLU99_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO16285.1"
                     /translation="MQTKKELAIRTRNSTLRPALRGRARGILTLCLLAIAAPAWSESA
                     DQSLTATIVGALAPDEVRLGETGLLDVHVRDMEIASLLEMLSYQARANIVTSTSVTGR
                     VSANLYAVNLEQALEAILTPNRYVFRRSGGTIFVATPEEIAALAPPPVTRVFKLCYIQ
                     PVEAAAAIKAVLGPNSSVVEGGKESTSGKSDSTGKELAKMGPAGVDYVIVTASPSELE
                     AVAQLLSEIDQRPRQVLVEATILRATLNETNELGIDFTLLGGVDFRNVSSTSNAAADL
                     QTGPLPPSRFEQSTLNVNTAFTQNVQGGGLTVGIINNSVAFFIRALEETTDVAVVANP
                     KTIALNKQEGEVIVGRRDGYLTTTVTETAAIQTVEFLETGTQIRFRPVINDDNTVRLS
                     VHPKDSNGGLTGANLPFEETTEARADLLVNDGGTVLIGGLFRERTVGGRSQIPVLGDI
                     PLAGALFGSRNNQTVREEVIILLTVHVLKDTPQEHEEFRRLLEDVERIRVGSRQGLMC
                     TGRERLAQAFYHEALRQLEQGDCDRALLNVRMALHNEPKLLPAIKLKEELLCRRMWDE
                     EGGRMRTFIWDLIRSDAPPDEPLPDFARPQPELELPQQSENECDDSQSANSDYAD"
     gene            5644..6282
                     /locus_tag="PLU99_04175"
     CDS             5644..6282
                     /locus_tag="PLU99_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO16286.1"
                     /translation="MAIRRIRESYKPKLRGFSMVELVLVVCIMAIVASMAVPRFANSL
                     TRNRVEAAARRVAADLSLARQRARMASASQSVVFDAAGNSYRLPGLSDPDHPGSEYVV
                     QLGAAPYEATLHRIDLGNDSLITFDAYGVPDKFGSVTVQCGEFVRVITVDAAGRATVT
                     DGAPSGTVLTGAAGSGEAANGTSAPDQGTSGKSLPDHASATAKGHSFLGSGG"
     gene            6293..7921
                     /locus_tag="PLU99_04180"
     CDS             6293..7921
                     /locus_tag="PLU99_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO16287.1"
                     /translation="MKYPCKYRAFTLVELVLSLAIATVLLGGMTSALLLATRALPGDT
                     RPVDVLTAQTQVAADICGELSYACHILERAPRAVSFTVPDRDGDGSPECIRYAWSGTP
                     GEPLTRTFNHGTPVTVLADVHEFALAYDVATVDETYPGPPVESAEISLSSRTSSLLTG
                     DCDLKEKAWGGQYFCPSFPAGTLSWKVTRAQVKARSEGAQKGVTAVQLRPATSSKTPD
                     TLVIDQALMYEKALQSNYRWEEFTFSNATGLAPGTALCLVLVCHTSDADLADVQYDRL
                     GSGLVHTTDAANWSYYDSLALLHDIRGKYMSPGATQTATRRYVTTVRIALRAGGDSQA
                     RVDSTARPVNAPEALSAVWESEFNTDPTLLDMNGDGLGDWAPATGVFDPATVSGGIWN
                     ATSNLVTAPDNDFTQLTTVELRFRCTSVCGSSPGVAFRINADRSNNSYVPLYAGLMLT
                     GGGTQTLMVYCMPDSGTRTSLLTLTGLPADFVDLRLLIDPARDTVNVRVNGGDCGTFT
                     YVLSPESGASRSATLLVSGAAAQFDRVRIRVGGN"
     gene            7921..8475
                     /locus_tag="PLU99_04185"
     CDS             7921..8475
                     /locus_tag="PLU99_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16288.1"
                     /translation="MSGSLHPRRHRHRPSLTLVEMVISTLIVSIMLVAALNAVGAARL
                     NECRLAENQRALLLAQALMAEILCQPYSAADEAGDVIGSADAAAAVTNRTGFNSVGDY
                     NGWRASPPQNRDGSIIPNCDDYTQTVRVVWVDPGTLTSESNTATGVKCIQVSIERSQR
                     QLVRLTAYRTAVWKDPVQVQGGGP"
     gene            8472..9437
                     /locus_tag="PLU99_04190"
     CDS             8472..9437
                     /locus_tag="PLU99_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOO16289.1"
                     /translation="MSGLSLQCRKSGLRRGSVYLAALGVAMLVTLIGLSGVLATRVGW
                     RNAALTAAALQADTAAQSLMDVVLYRLTHDLYWRSHYQNDTWTAFEICGDGVTGSFKL
                     VDELDGDLANNESHPARLYCRATVGEAMRMYSIVFTAVPPQTNNLLNNPGMESGAANW
                     KGDRCTLAVAKDTSHSGGASLKATARDDHYAGPAQTVSGFLASGQSYEVEAWVKTDTG
                     TADAKLVLYCNGLLSLSTYYAGGTTTVNAGSWTRLSATLTPSWSGTLLDAYLKINTTS
                     GTVSFYVDDVIMRKAGTGRDAELTPMPGTFRREVLAEPAASLMQT"
     gene            complement(9566..10903)
                     /locus_tag="PLU99_04195"
     CDS             complement(9566..10903)
                     /locus_tag="PLU99_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOO16290.1"
                     /translation="MKRVLVLGAGLVSRPLVRYLLNQSDFQVTVASRTVSKADALVGG
                     HPRGSTTDLLADDHEKLERLIEDHDLAISLLPAPLHPVVAECCLRHRKHMVTTSYVSP
                     KMRSFDEAARKADLILLNEIGVDPGIDHMSAMRVIHGVRQRGGEVVSFKSYCGGLPAP
                     EANDNPWGYKFSWAPRGVCTAGKNPAKYRENGKQIDIPGPELFLNHHPVPVQGVPGHT
                     ELEGYPNRDSLIYLDLYGLKNVETMFRGTLRYPGWCKTLKAVVDLGMLDESPVTYAKG
                     TTFAQWTAGLIKADSPVNIRAKVAKFLKMDEKANPLERMEWLGLFSNDPLPIIGQQTT
                     VLDILATRMNQKMTYAPGERDMIVLLHRFVARFPGKPDERISSTLIDFGQPDGDTSMA
                     RTVSLPAAIATRLILTGRIVERGVWAPVTPGIYEPVLNELESMGIRCVEKTEG"
     gene            complement(10972..12270)
                     /locus_tag="PLU99_04200"
     CDS             complement(10972..12270)
                     /locus_tag="PLU99_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HOO16291.1"
                     /translation="MIIGIRREDKSEWEGRVPLVPNDIRWLHEEHGIDFRVQTSPIRA
                     IKDDEYRSCGAAVVDDLSDCPVIMGVKEIPVERLAPGKTYVYFSHVIKGQPHNMPALR
                     RLMELGCTLIDYERIVDDQNRRLVFFGRYAGLAGMIDTLWAFGQRLRHEGIDSPFAQV
                     RPAHRYTDLESVKREFAGIADAIRARGLPEAINPLVCGFTGYGNVSQGAQEIYDLLPV
                     EEITPQQLASVRPNPHCCYKVVFREEHIVTRIDASAPFELQEYYRHPERYRGVFFQYA
                     PHLSLLVNCIYWEEKYPRLITREQFKELWNAGQPRLRVIGDISCDIDGSLACTTRATD
                     PAAPVYVYDPFTGETIDGVAGRGPVVLAVDFLPCELPIDASNYFSRTLRPFIPALARA
                     DFSAPLPGSGLPPELQRATIVYRGRLTEAYRHLEQHLHQA"
     gene            complement(12764..13405)
                     /locus_tag="PLU99_04205"
     CDS             complement(12764..13405)
                     /locus_tag="PLU99_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOO16292.1"
                     /translation="MLSKRSVVAWSVPVILGLLGWANVSLAQEKKPEPPKPEPKKMEP
                     PKKEEPKDEVKDIAATLKTNPDLKQFERLFSASGVDLKGGEFTIFAPTNDAINKKGNL
                     WVQNLLKPEQKSELKKVVESYIVKGKMTATQLKNQKAPLKSMTGEALTITEKDGKLTV
                     NGANVVKTEITAKNGVIHLIDQVLEEKGAKAEPAPKPVPAPKPEQPKPETPKP"
     gene            13844..15145
                     /locus_tag="PLU99_04210"
     CDS             13844..15145
                     /locus_tag="PLU99_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOO16293.1"
                     /translation="MQNSELTQNLRALCLPGGRLSGTDNAFIAERYVLDKAREYGLRN
                     GHLEAFAMPCWRATYTRVVRLADQEIPCEGALALCNTMSTPPGGISAPVLDVGDGNQE
                     DFDLLNGKLNGRFALVRDGGRNRRSEKMDRALEHGAAGLVVISAADRAPVIGTAHHTP
                     RPEPAVIISHEDGQTIAQALAAGETVQLNIQISSESWDGEPHNAIAEIPGHGPHRDEM
                     ILVCAHLDSWHLAEGAIDNANGSAVILECARALRAVDWRPRRTVRFIWFMAEEQDLRG
                     SRAYVAAHQDELDRVVAMINIDMPGSPRALWTSGPPQFVERLQQFRRGMAGYLLNEDI
                     GRLSGTWSDHAPFVEAGVCGVTLGGELGPGGRNYHTANDKFECVDQRATIESAAVLAV
                     LVRQLADDLSLKPGRCPPDEPPASALTPASATQSARLAGRH"
     gene            complement(15051..15515)
                     /locus_tag="PLU99_04215"
     CDS             complement(15051..15515)
                     /locus_tag="PLU99_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOO16294.1"
                     /translation="MDTNAVKWLRSQNVQFTVLEYQFTEIGAEHAAEAVGRPLEMVCK
                     TLIVKTSGNAYWVAIVPGDQRFDTRLMAAAVGAPRADLAEQDEAEKVTGYKVGGISPF
                     AMRRRLPVVIEESLLALDSIIVNGGRRGVLIELPTPELVRLLEARPADICRA"
     gene            complement(15603..16613)
                     /locus_tag="PLU99_04220"
     CDS             complement(15603..16613)
                     /locus_tag="PLU99_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16295.1"
                     /translation="MQPGPTFDEQLLDLHLGRLSASEQVELNLRMAADAGLQQQHQTL
                     TAAFKALHEYKADPAPPDLATRVAARVAAARRPVRLIRPADDLTRTVERGAPVIVRLG
                     NLREIVAAAAMIVLMVGVAAPSLLHLRERGQRMACSANLAALGMGVQQYAATYGSSLP
                     FTGWNSRASWAPSQDPGVMLVPNRRHLYPLLQKDFIAHPRLFLCPSNGGAPMPIDQVH
                     RCNDFLDDANLSYTYFNMAGRRPSSEDNPDLPVMSDQNPVFGESTSFLGKFAFRDRAQ
                     SNSPSHGGAGQNILVLGGYVKWVKTPDAGVDGDNIWTLQGVSTYTGREGPASAADAHL
                     IK"
     gene            complement(16634..17227)
                     /locus_tag="PLU99_04225"
     CDS             complement(16634..17227)
                     /locus_tag="PLU99_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO16296.1"
                     /translation="MDARRDEELLADHLAGKKGAFDILVQRYADELFAFLHRFVGNAA
                     AADDLVQESFLQVHLAADAFDPQRAFRPWLYTIAANKARDYLRGRGRRLEQSLDARGP
                     DDDGLGPDELVPAAEESSFEQSSAEEVRAAVRSVIAKMPEHLQLILMLGYFQRLPYSD
                     IAEILDIPVGTVKSRLHAAVARFAWMWHKQMRPERQR"
     gene            complement(17565..>18477)
                     /gene="pdxA"
                     /locus_tag="PLU99_04230"
     CDS             complement(17565..>18477)
                     /gene="pdxA"
                     /locus_tag="PLU99_04230"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOO16297.1"
                     /translation="GLHDVLAVAADAAEINPFWWREPFEAGARVGSGVLVADFDELPA
                     VRVNGTRGPEALAGEASYRFVEAGIRSLKAGLIDALVTAPISKEAWKLAGHRAVGHTH
                     LLSDMFETRRVTMAFVADELRVALASAHVPLFELRNRFTIGLVHQPIDLLDRALREWF
                     GIPRPRIGVLGLNPHAGENGMLGDEEQRIIEPALAMARSAQIDVTGPLPPDTAFTPEV
                     AGQFDGLVAMYHDQGLIPLKALAFHRAVQLTLGLGAVRTSPDHGTAFDIAGRNRANPG
                     SMRAAIRLAIDLARTRLAQRASAAELT"
BASE COUNT         3323 a         6075 c         5597 g         3482 t
ORIGIN      
        1 ccggccggtg ctatgtgacc gacagccgtc gcacctacga cgtcgacggc ggtccgacgc
       61 acttgacttc gccggccttc gatatgagcg ccatgcacag cccgatcgtt cgcttcgccg
      121 agttcttctt ctgtgatgac gcgacggtct atccgccgac gcaggacttc ctggacgtgc
      181 acctgtccag cgacggcggc gccacctggg tcctggccgc gcaatatgcc agccatgatg
      241 actgggcgat gcacgagatt cgcgtggccg acttcatccc gctgacggcg accgttcagg
      301 tgcggttcac ggccaacgat actccgaata actcgcagac cgaggccggc atcgacgccg
      361 tctggatcta cgatcttgcc tgcgacgcgc cctggaagct cggcgatgtc aactgcgacc
      421 aggccgtcaa cgtgtttgac attgacccgt tcgtgctggc cctaaccgac caggccgaat
      481 acgttgcgac gtaccccgac tgcaacgtca tgaacgccga cacaaacggc gatggggcga
      541 tcaacgtgtt cgacattgat ccgttcgtca atctgctgac tggcgcacag ccctagggtc
      601 gaacggcgcc tggcccccgc gtgtgccact gcttgaagcc gcagcttcaa gcagtgccgc
      661 tggccgtgca cccgcgacga cgtgacccgc gatgacgtga taaggtgata aggtgataaa
      721 gtgatagggt gataaaggcc gacgcgcgga tcctttatca ccctatcact ttatcgctct
      781 atcactctct acgtcgagcg gctcccgtcc ggggcgacgc tgccggtgtg agtctcctgg
      841 gcctgcttag cgcccagcag cgcctgcatg atctggtcgg agaaagcgct catcccggcc
      901 gggctatagg gcaccatcat gatggttgcg ccgctgcgcg agccgaccgc ggtgaccgag
      961 tcgtagtgct gcgtgagcag caccatcttc gtcgcctctt cgggcgaaat gccggcttcg
     1021 gagcacgatt cgaccgactc cttcagaccc ttggcgatcg ccagccgctg gcgcgcgata
     1081 cccacaccct ggagttcctt ggcccgggcc tcggcctccg cctcggcaat aacgcgaatc
     1141 ttcctggcct cggcttcgtt cttggcagcc tccttcaggc gggcgctggc gttgaccttg
     1201 ttcatggccg ccttgacttc ggggtcgggc tgaatgtcgt tcaccagggc ctgaagaatc
     1261 gtgtagccga agtcgtccat aatctgctcg agccgctcct tgaccgccga cgcgatcttc
     1321 tctttctcgg agaagacgtt gtcgaccggc atttcgggaa ccagggcgcg gaccgtgtcg
     1381 aacacgtagg acgaaatctg ctgttgcggg ttcatgagct tgtactgggc ggatttgacg
     1441 gcttcctccg tctctcggac gaagaactgc accgcgatca gcagctccac gaagacgtca
     1501 tccttggttt tcgtttcgac tttaatctcg agctcgcgca cgcgatgcgt gatccgcccg
     1561 gccaccgtat caacgagcgg ccagcggaaa tgcaaccccg ggccgcagat gcgcaggaaa
     1621 cgcccaaagc gctgcaccac cgcgtgtgtc tgctgttgga cggtgaagaa gccgctcagc
     1681 agcaccagca ccactacaac aacaattact ccaaagacca tggcgggcat gcttttctcc
     1741 gttgcaaaga ctgcggtcta gttttccgca tcaaactcag ccgattcgag cgccagcgtt
     1801 aactcgtgcg gctcgtcggg taattcccag gacgggactt ccagcgtcac ggtctgacct
     1861 cggccccagc gctccaccgc gtgcggaatg cagaaagcgg cttcgccggc cgggccgtga
     1921 cctcgaatta atattataac atcgacaagc gagtagccgg cgttgaaggc gacgatgatc
     1981 cgcggctcag tgtcgctgag ggcttgaccg ccccacgccg acttcatgct gaaaaaaaga
     2041 tcaggcgtgt catcgaagca ttggaaaccc ggcggcgcag ccaacggttg cgggtggcgt
     2101 atctggcccg gcccgagcag cgagccgtcc ggcccgcgca ccagagccag gccgcaacga
     2161 cggcagaagc gcgccacctc cgggttggca attcgacaac gtgggcaggc caactccatc
     2221 atcacttccc caaaagccag ctaactgaat tctacaccga aatcaggttc aggggtacgg
     2281 aacgtgcagc gtcgcgcccc aagcaatcgt cgattctagt aagaacccag cgacagaatg
     2341 tgaagcgcaa ccgggttggc tgggtcgatc tcccgcagac gacgcgccct ctcccgcgct
     2401 ctgctttcct gcccagcgct ccactccaag gctgccgtca attcctgaac gcgtgcgtcc
     2461 tgcggctgga gttgtagtgc ccgctccgcg gtacgcagcg ccgcaccggt gtccccggcc
     2521 gcggcccaag cctgcgccag cagcagcaac gcggagacct ggttcggatc gctctgcagc
     2581 accgcctggg cttgcacccg ggctgcctcg ggctggttga cagccagcag cgcttccgcc
     2641 aggcgcaggc gtacgccggt tgactgctgg gcttgctctg cgttcagcaa ttgctgcaac
     2701 acgggaatcg cctcaccata gcgcccagcc cggcatagcg cctccgccag ccgttcgctg
     2761 atgccggcgt catcgtccgc agcctgcaaa acgcgccgcc aggccgctgc cgcgccgctc
     2821 caatcgcccg cgagctccag agcctctgcc aaagccgcct gataagcatt cgtccaggcg
     2881 aagttcgtgg cgcaagcttc caaacactgc agcgcttgcc gcggctgccc aagttgcagc
     2941 catacctgga ccgccgcgac tacatagccc acttcgcttt ggtccagttc cgccgctctt
     3001 tcgaaagccg ctaccgcgtc ctcccagcgt tgctgttgct gatacacgat gccgcgcaga
     3061 tattcgactt ccgcttgtac gggaccgtcg agctgcgtgc gatccaacag gtcggccgcc
     3121 gcggccgtgt tcccttccgc cagccagatg cgggcctgga gcggcgtcca gttcatgttt
     3181 gcgggatcga gtcgccgagc ctcggccagc tcaccggctg cggctttgac attgccggcc
     3241 gcgaattggt cgtacgccag tttggccttg agctgcgcgc gagcctggtt ccaccgttgg
     3301 gttgcttcct gattggcctg ttgctggggg gactgacagc cggccagact caacacgagc
     3361 gccgccagca ccggcgccca gagggcgaac cttcgggcgc gtcgcggctc aacaggagcc
     3421 tcgccctccc aaggcattcc gcgaagcttc ctgcggtcag tcagcataat cggaattcgc
     3481 ggactggctg tcatcgcact cgttctcact ctgctgaggc aactcaagtt caggctgcgg
     3541 ccgggcgaag tccggcagcg gctcatctgg aggcgcgtcg ctccggatca aatcccagat
     3601 gaacgtgcgc atgcggccgc cctcctcgtc ccacatgcgc cggcacaaca gttcctcctt
     3661 gagttttatc gccggcagca gcttgggttc gttgtgcagc gccatccgca cgttcagcaa
     3721 cgcacggtcg caatccccct gctcgagttg ccgcaacgcc tcgtggtaaa acgcctgtgc
     3781 cagccgctcg cgccccgtac acatcagacc ctggcggctg ccgacgcgta tgcgttcaac
     3841 gtcctcgagc agccggcgga actcctcgtg ctcctgcggc gtgtctttca gaacgtgcac
     3901 tgtcaggaga atgatgactt cttcacgcac agtctggttg tttcggctgc cgaacagcgc
     3961 gcctgccagc ggaatgtcgc ccaaaaccgg aatctggctg cggccgccca cggtgcgttc
     4021 acgaaacaaa ccgccgatga gtaccgttcc gccatcgttg accagcaaat ccgcccgcgc
     4081 ttccgtcgtc tcctcaaacg gcagattcgc gccggtcagc ccgccgttgc tatccttggg
     4141 atgcaccgac aggcgcaccg tattgtcatc gttgatcacc ggccgaaaac ggatttgcgt
     4201 gcccgtctcc agaaactcga cggtctgaat cgccgccgtc tccgtcaccg tggtcgtcaa
     4261 gtacccgtcg cgccggccga cgatgacctc gccctcctgc ttgttcagcg cgatcgtctt
     4321 gggatttgcc acgacggcga cgtccgtggt ttcttccaac gcgcggatga aaaaggccac
     4381 gctgttgttg atgatcccca ccgtaaggcc gccgccttgc acgttctgcg taaaggccgt
     4441 gttcacatta agcgtgctct gctcgaagcg gctcgggggc agcgggccgg tttgcaggtc
     4501 agccgcagca ttgctggtgc tggaaacgtt gcggaaatcc acgccgccca gcagcgtaaa
     4561 gtcgatccca agttcattag tctcgttcag cgtggcgcgc agaatcgtcg cttcgaccag
     4621 aacctgccgc gggcgctgat cgatttcgct caggagctgc gccaccgctt ccaattcaga
     4681 cgggctggcc gtcacaatga cgtagtccac gccggccggg cccatcttcg ccagttcctt
     4741 gccggtggag tcgctctttc cgcttgtgct ctcctttccg ccttcgacca cgctgctatt
     4801 aggcccgaga actgctttta tcgcggcggc cgcctccacc ggctggatgt agcacagctt
     4861 gaacacgcgt gtgaccggcg gcggggcgag cgcggcaatt tcctcgggtg tggcgacgaa
     4921 aatcgtcccg cccgacctac gaaagacgta gcgattcggg gtgagaatgg cctccagcgc
     4981 ctgctcgaga ttgaccgcgt aaagattggc cgaaacgcgc ccggtcacgc tcgtgctggt
     5041 gacgatgttc gcgcgggcct gataactcag catttccagc agcgacgcga tctccatgtc
     5101 ccgcacgtgc acgtccagca gcccggtttc gcccagacgc acctcgtcgg gcgccagtgc
     5161 gccgacgatg gtcgcggtca ggctctggtc tgcagactcg gaccacgctg gggccgcgat
     5221 cgccaacagg caaagcgtca ggattccacg ggcgcggccg cgcaaagccg gacgtaacgt
     5281 gctattcctg gtccgtattg ccagttcttt cttggtttgc attcctggcg cagttccttg
     5341 ctgtggcgtc cgcccctggc ggcggactgg gcctcagaag gcggacggca ccaccggggg
     5401 gccgacgcta cagaccggcc ggcgtgattg atggaggccg gcttcttctc aattagatcg
     5461 gagatgcttt atcggaggat tcaatccatt tgcccgactg tatgcgcctg aaatatccca
     5521 aacccgctgc cccgagcggt cgatgatgct gttgatgcgc cgtcccatgc gcttttgcga
     5581 acggccggcg gcaagggcgg cttggcttcg ttctccatac cgtgagtgat gaatacccgt
     5641 cagttggcaa ttcgtcgaat tcgcgagtcc tacaagccga aattgcgcgg cttcagcatg
     5701 gtcgagctgg tgctggtcgt ctgcatcatg gccatcgtgg ccagcatggc tgtgccgcgt
     5761 ttcgcgaatt ccttgacgcg caaccgggtc gaggcagccg cgcggcgggt ggccgccgac
     5821 ttgtcgctgg ctcgacagcg tgcccgcatg gcaagcgcct cgcagagcgt cgtctttgac
     5881 gcggccggca acagttatcg tctgcccggt ttgtctgacc cggatcaccc gggttcggag
     5941 tacgtcgtac agcttggggc cgccccctac gaggccacgc tgcacaggat cgatctgggc
     6001 aatgattcgc tgattacttt cgacgcgtac ggcgtgcctg acaagtttgg gtccgtaacg
     6061 gtgcaatgcg gcgagttcgt gcgcgttatt acggtcgatg ccgccgggcg cgctacggtc
     6121 accgacggcg caccgtccgg cacagtgctt accggcgcgg caggctcggg cgaagcagct
     6181 aacgggacgt cggcgccgga ccagggaaca tcaggcaaat cgctgcccga tcacgcgtcg
     6241 gccacagcca agggacattc atttctcggc tctggtggct agggcagtgc cgatgaagta
     6301 tccgtgcaag tatagagcgt tcacgctggt tgaattggtc ctcagcctgg ctattgccac
     6361 cgttttgctg ggcggtatga cctcagccct gctcctggcg acgcgcgccc tgccgggtga
     6421 cacgcgcccc gtggatgtac tcaccgccca gacccaagtc gcagccgaca tctgcggcga
     6481 actgtcttac gcgtgccaca tcctcgaacg cgcgccgcgc gccgtcagct ttaccgttcc
     6541 cgatcgcgac ggtgacggct cacccgaatg catccgctac gcctggtccg gtacgcccgg
     6601 tgagccgctc acccgaactt tcaaccacgg tacgcccgtc actgtcctgg cggatgtgca
     6661 cgaattcgcc ctggcctacg atgtggccac ggtggacgaa acatatcccg gtccgccggt
     6721 ggagagcgcg gaaatcagcc tgagcagccg caccagcagc cttttaacag gagactgcga
     6781 cctcaaagag aaagcttggg gggggcagta cttttgtccc tcgtttcccg ccggcacgtt
     6841 gagctggaag gtcacgcgcg cgcaagtgaa agcgcgctcc gaaggcgctc agaagggcgt
     6901 gaccgctgtg caattgcggc ccgcgaccag cagcaaaacg cccgatacgc tggttattga
     6961 tcaagccctc atgtacgaaa aagccctgca gtcgaactac cgctgggagg aattcacgtt
     7021 cagcaacgcc accggcctgg cgccggggac cgccctgtgc ctggtcctgg tctgccatac
     7081 ctcggacgcg gaccttgcgg acgtccagta tgatcgcttg ggcagcggct tagtccacac
     7141 tactgatgct gcgaactgga gttattacga ttctctcgca ctgctgcacg acatccgcgg
     7201 caagtacatg tcgccgggcg cgacgcagac cgccacgcgc cgctacgtca cgacggtgcg
     7261 gatagccctg cgggcgggcg gcgactccca agcccgcgtc gactcgactg cgcgccccgt
     7321 taacgccccc gaagcgttgt ccgccgtttg ggaatcagaa ttcaacactg acccgacgct
     7381 tctggacatg aacggcgatg gtcttggcga ctgggccccg gccaccggcg tcttcgatcc
     7441 ggccaccgtg agcggcggca tctggaacgc gacctccaat ctggtcaccg cgcccgataa
     7501 tgacttcacg cagttgacca cggtcgagct tcgtttccgc tgcaccagcg tgtgcgggtc
     7561 ctcgccaggg gtcgcctttc gcatcaacgc ggatcgctcg aacaacagct atgttcccct
     7621 gtacgccggc ctgatgctca ccggcggcgg cacgcagacc ttaatggtct actgtatgcc
     7681 tgattccggg acgcggacca gcctgctgac gctcacgggc ctgccggcgg acttcgtgga
     7741 cttgcgtttg ctcattgatc ccgcgcgcga caccgtcaat gtgcgagtca acggcggcga
     7801 ctgcggaact ttcacatacg tgttgtcgcc cgaatccggc gcgtcacgtt cggccacact
     7861 gctcgtatcc ggggctgcgg cccaatttga ccgcgtgcgc atccgcgtgg gaggcaactg
     7921 atgagcggct cgctacatcc ccgccgccac agacaccggc ccagcctgac gctcgtggaa
     7981 atggtaatct ccacgctgat tgtgagtatc atgctcgtgg ccgccctgaa tgcggtcgga
     8041 gcggcccgtc tcaacgaatg ccgtctggcc gaaaaccagc gggcgctgtt gctggcgcag
     8101 gctctgatgg ccgagatact ctgccagccc tacagcgctg cggacgaggc gggagacgtg
     8161 atcggctcgg ccgacgccgc ggcggccgtc accaaccgta ccggcttcaa cagcgtgggc
     8221 gactataacg gctggcgcgc cagtccgccg cagaatcgag acggctcgat catccccaac
     8281 tgcgacgact atactcagac cgtgcgcgtg gtatgggtgg atcctggcac attgacttcg
     8341 gaatcgaaca ccgcaaccgg tgtgaaatgt attcaagtga gtatcgagcg ttcgcagcgc
     8401 caactggtcc ggctcacggc ctatcgaacg gcggtctgga aagacccggt ccaggtacag
     8461 ggaggcgggc catgagcggc ttgtcgctgc agtgccgaaa gtccggcctc cgccgcggca
     8521 gcgtttacct cgccgcactg ggcgtcgcga tgctcgtcac actgatcggt ctttcgggcg
     8581 tgctggccac gcgcgtcggc tggcgcaacg ccgcgctcac ggccgcggcc ttacaggccg
     8641 atacggccgc acagtcgctt atggacgtgg tgctgtaccg cctgacgcat gacctctact
     8701 ggcgctcaca ctaccagaac gacacttgga ccgcgtttga gatctgtggc gatggcgtga
     8761 cgggcagctt caagctggtg gatgaattgg acggcgacct ggcgaacaat gagagccacc
     8821 ccgcgcgcct gtactgccgc gcgaccgtgg gcgaagccat gcgcatgtac agcatcgttt
     8881 tcaccgcggt accgccgcag accaacaacc tgctcaacaa cccgggaatg gagagcggtg
     8941 cggccaactg gaaaggtgac cgctgcactc tggccgtcgc caaagacacc tctcacagcg
     9001 ggggcgcgtc gctgaaggcc acggcccgcg acgaccatta cgccggcccg gcgcagacgg
     9061 tttccggctt cctggcgagc ggccagagct atgaagttga agcgtgggtc aagacggata
     9121 ccggcacggc ggacgccaag ttggtgttgt actgcaatgg cttattgtcc ttgtccactt
     9181 actacgcggg cggcacgaca acggtcaatg ccggcagttg gacgcggctg tcggcaacgc
     9241 tgaccccgag ctggtccgga accctgctcg acgcctatct caaaatcaac accacatcgg
     9301 gtacggtgtc attctacgta gacgacgtca tcatgcgcaa ggccggcacc ggccgggacg
     9361 ccgaactcac gcccatgccc ggcactttcc ggcgcgaagt cctggcagag ccggccgcgt
     9421 cattgatgca aacgtaggcc gggcccgcag acccgacatt cccacgccgg aggcgctcag
     9481 caagcgaact cggcaaacga acgtcggctc tgcggacccg gcctgttcct ctgcggatcc
     9541 cggtctactc agtctaggtt tacagtcagc cctcagtctt ctccacgcac cggatgccca
     9601 tgctttccaa ttcgttcaaa accggctcgt aaatgcccgg cgtcaccggc gcccacacgc
     9661 cgcgctcgac gatgcggccg gtcaggatga gccgcgtggc gatggccgcc ggcaggctca
     9721 ccgtgcgggc catggacgta tcgccgtcgg gctggccgaa atcgatcaaa gtcgagctga
     9781 tgcgttcgtc gggcttgccc gggaaacgcg ccacgaagcg atggagcagc acgatcatgt
     9841 cgcgctcgcc cggggcgtag gtcatcttct ggttcatgcg cgtggccaga atatcgagca
     9901 cggtggtctg ctgcccgatg atcggcaacg gatcgttcga gaacagtccc agccactcca
     9961 tacgctcgag cggattggcc ttttcgtcca tcttcaggaa cttggcgacc ttggcgcgaa
    10021 tattcaccgg cgaatcggcc ttgatcagcc cggcggtcca ctgcgcgaac gtcgtaccct
    10081 tcgcatacgt gaccgggctc tcgtccagca tgcccaggtc aaccaccgct ttcagcgtct
    10141 tgcaccagcc ggggtagcgc agcgtgccgc ggaacatcgt ctcgacgttc ttcagaccat
    10201 agagatcaag gtaaatcagc gaatcgcggt tgggatagcc ttccaactca gtgtgcccgg
    10261 gcacgccttg caccggcacc gggtggtggt tcaggaacag ctcggggccg ggaatatcga
    10321 tctgcttgcc gttttcgcga tacttcgcgg gattcttgcc ggcggtgcag acgccgcgcg
    10381 gcgcccacga gaacttgtaa ccccacggat tgtcgttcgc ctcaggggcc ggcaggccgc
    10441 cgcaatacga tttgaagctc accacctcac cgccgcgctg ccgcacgccg tggatcacgc
    10501 gcatggccga catgtggtcg atgcccggat ccacgccgat ctcgttcagc agaatcaaat
    10561 cagccttacg cgccgcctcg tcgaagctcc gcatcttggg gctgacgtag gacgttgtaa
    10621 ccatgtgctt cctgtgccgc aggcagcact ccgcgaccac cgggtgcagc ggcgccggca
    10681 gcaggctgat cgccaggtca tggtcctcga tcaaccgttc gagcttctcg tgatcgtcgg
    10741 ccagcagatc agtcgtgctg ccgcgcgggt ggcccccgac cagcgcgtcc gccttgctga
    10801 cggtgcgact ggcgacggtg acctggaagt cggactgatt gagcagatag cgaaccagcg
    10861 ggcgactcac caagcccgca cccagcacga gaactcgttt catcgattgc ctcctgaggc
    10921 tatatcacgg ccgataaacc agtggaatct cgcttcgcat cccacgtcgg cttaagcctg
    10981 atgcaagtgt tgttccaggt ggcggtaggc ctccgtcaac cgtccgcggt acacaatagt
    11041 cgctcgctgc aactccggcg gcagcccact tcccggcagc ggtgcggaaa aatccgcccg
    11101 cgccagggcc gggatgaacg gcctgagcgt gcggctgaag tagtttgagg cgtcgatcgg
    11161 cagctcgcac ggcagaaaat cgaccgcgag cacgaccgga ccgcggcccg caacgccatc
    11221 tatcgtctca ccggtgaagg ggtcgtacac gtaaaccggc gcagccggat cggtcgcgcg
    11281 cgtcgtgcag gccagcgagc cgtcgatgtc gcacgaaata tcgccgatga cgcgcagccg
    11341 cggctggccg gcgttccata attccttgaa ctgttcgcgc gtgatgaggc gcgggtactt
    11401 ctcctcccag tagatgcagt tcacgaggag gctcagatgc ggggcgtatt ggaagaacac
    11461 gccgcgatag cgctccgggt ggcgatagta ctcctgcaat tcgaacggcg ccgaggcgtc
    11521 aatccgcgtc acgatgtgct cttcgcgaaa cacgaccttg tagcagcagt gcggattggg
    11581 ccggacggac gcgagctgct ggggcgtaat ttcctccacc ggcaggaggt cgtaaatctc
    11641 ctgtgccccc tgcgacacgt tcccgtagcc ggtgaaaccg cagacgagcg gattgatcgc
    11701 ctcgggcagc ccgcgggcgc ggatcgcatc ggcgatgccc gcgaactcgc gctttacgct
    11761 ttcgagatca gtgtaccgat gcgccggacg cacctgcgca aaaggactgt cgatgccctc
    11821 gtgccgcagg cgctgaccga aggcccatag cgtatcgatc atgccggcca gccccgcata
    11881 gcgcccgaag aacaccaggc gcctgttctg atcgtcgacg atgcgctcgt aatcaatgag
    11941 cgtgcagccc agttccatca ggcggcgcag ggcgggcatg ttgtgcggct ggccttttat
    12001 tacgtgtgag aagtagacgt acgttttgcc gggcgccagg cgttcgacgg gtatctcctt
    12061 aacgcccatg atgaccgggc aatcggagag atcgtcgaca acggccgcac cgcaagaacg
    12121 atattcgtcg tcctttatgg cgcgaattgg agaagtctga acgcggaagt cgatcccgtg
    12181 ctcctcgtgc agccagcgga tgtcgttggg caccagcggg acccgcccct cccactcgct
    12241 tttgtcttcg cgtcgaattc cgatgatcaa ggtcgtaact ccttcctgtc ccggcgcgac
    12301 gtcccgcgcc gatgctgttg gcgcagagcc gctcgctggc ggcgagcccc tcatcgtagg
    12361 gcgcagccgt ccgggcaaca aggtggcggc ttttcaactt ccgtggccga tggagctacg
    12421 tacgcttacg tgaatgtgca acggctatgt cggacagatg tcagcagaag ggaggcgcat
    12481 gctcgcgtct gctcaccagc gcataaggaa atcgctatgc ctaacgagtc agcagccgct
    12541 caggctgcca cgcctccgcc agccacgcgc cgcaccttcc cacgttccca ctttcccact
    12601 ttcccacggt cacgcgtgga aacgtgggaa ggtgggaagg tgggaaggtg tgtacacctg
    12661 gaggtaccga ggtgtcttga gcaagccaga tcgcgcaaaa aaagaaaccg agggtccgcg
    12721 aagaacggac cctcagctca tcaattcatg ggcagactga agcttacggc ttcggcgtct
    12781 cgggcttcgg ctgctcgggc ttcggagcag gaaccggctt cggggccggt tctgccttgg
    12841 cgcctttctc ctcgagaacc tggtcaatca ggtgaatgac accgttcttg gccgtgatct
    12901 cggtcttgac gacgttcgcg ccgttgacgg tcagcttgcc gtccttctca gtgatcgtca
    12961 gcgcctcgcc ggtcatgctc ttgagcgggg ccttctgatt cttcagttgg gtggccgtca
    13021 ttttgccctt gacgatgtag ctttcgacga ccttcttcaa ctcggacttc tgctccggct
    13081 tgagcaggtt ctgcacccac agattgccct tcttgttgat ggcgtcattg gtcggtgcga
    13141 agatggtaaa ctcgccgccc ttcaggtcga cgcccgaagc gctgaacaga cgctcaaact
    13201 gcttcagatc cgggttggtc ttcagcgtcg cggcgatgtc cttgacttcg tccttgggct
    13261 cttccttctt gggcggttcc atcttcttcg gctcaggctt cggtggctcg ggcttcttct
    13321 cctgggccag cgaaacgttc gcccagccga gcagacccag aatcaccggg accgaccaag
    13381 caaccactga cctcttacta agcatctgaa cgttctcctt cttctgaatc cccggtcggt
    13441 acggcgcggt agcattccca acgcaggaat gccaccccga cggttttcga ccacatcgaa
    13501 gcgacacaac ctatcacgcg acaatacttt tgcaaggaat tttcattatc ggcacgcgcc
    13561 atagcaaaac acatgccacg attccgcact tctggccttg agcacctccg gtcaaatagc
    13621 ttagtctgtt ccctggccgt tagtgccggt ggcttaaggg tttacgctta tttaacgcgc
    13681 aaggacttac tccgtggatg cccaattgcg acatatactc tggacgaatg accatttgcg
    13741 actattctgc tcgcttctgt tgtggtttac aattccttac gtttcaaccg gctgctttag
    13801 cccgcacccc acgcacttag atcgcctcat tataggtgaa gttatgcaga actcagagct
    13861 gacacagaac ctgcgcgctt tgtgtctgcc cggaggccgg ttgtctggaa ccgacaatgc
    13921 gttcattgcc gagcgctatg tgctcgacaa agcccgggag tacggattgc gcaacgggca
    13981 cttggaggcg tttgccatgc cgtgctggcg tgccacttac acgcgcgtcg tgcggttggc
    14041 cgaccaggag attccctgcg agggcgcgct tgccttatgt aacacaatgt ctactccgcc
    14101 tggcggcatt agcgcgccag tcctggatgt cggcgatggc aaccaggaag atttcgacct
    14161 gctgaatggt aaactgaacg gccggttcgc gctggttcgt gacggcggcc gcaaccgccg
    14221 ctccgaaaaa atggaccggg cactggagca cggcgcggcc gggctggtag taatcagcgc
    14281 cgccgaccgt gcccccgtaa tcggtaccgc ccatcatacg ccccgccccg agcccgccgt
    14341 gatcattagt catgaagacg ggcagaccat cgcgcaggca ttggcagccg gtgagaccgt
    14401 gcagctcaac atccagatta gcagcgaatc ctgggatggc gagccgcaca acgccatcgc
    14461 ggaaatcccc gggcatggcc cgcaccgcga cgaaatgatc ctcgtctgcg cacacctcga
    14521 tagctggcat ctggccgaag gcgccattga caacgccaac ggctcggccg tcatcctcga
    14581 atgtgcccgg gccctgcggg cagttgactg gcgcccgcgc cgcactgtgc gcttcatctg
    14641 gtttatggcc gaagagcagg atttgcgcgg cagccgtgcg tatgtggccg ctcatcagga
    14701 tgagttggac cgggtcgtcg cgatgatcaa tattgacatg cccggctcgc cgcgtgcact
    14761 gtggacttcg ggcccgccgc agtttgtaga acggcttcag cagttccggc gcggcatggc
    14821 gggttacctg cttaacgagg atatcggcag actatccggg acctggagcg atcatgcgcc
    14881 attcgtcgag gccggcgtgt gcggcgtgac gcttgggggt gaactgggtc cgggtggccg
    14941 gaactaccac accgcgaatg acaagttcga gtgcgtcgat cagcgcgcga cgatcgagtc
    15001 ggccgcggta ctggcggtgc tggtgcggca actggccgac gatttgtcac tcaagcccgg
    15061 cagatgtccg ccggacgagc ctccagcaag cgcactaact ccggcgtcgg caactcaatc
    15121 agcacgcctc gccggccgcc attgacgatt atggagtcga gtgccagcag agattcctca
    15181 atcaccacgg gcagtctgcg gcgcattgca aacggcgaaa ttccgcccac tttgtagccg
    15241 gtgacctttt cggcctcgtc ctgttcggcg aggtcggccc gcggcgcccc cacggcggcc
    15301 gccatcagac gtgtatcgaa gcgctgatcg cccggaacga tcgcgaccca gtaggcgttg
    15361 ccgctcgtct tcacgatcag cgtcttgcag accatctcga gcggccggcc gacggcttcg
    15421 gcggcgtgct cggcgccgat ctcggtaaac tgatattcga gaaccgtgaa ctggacgttc
    15481 tgcgagcgca accacttgac ggcgttcgtg tccatatcag agccggctcg tgtcgagccg
    15541 gaatgatgat actgcgcctc gctgccccag caccaccccg tgtaaagcag aaccccgcaa
    15601 cgctatttga tcaaatgcgc atccgccgcc gaagccggcc cctcgcgccc ggtgtacgtg
    15661 ctcacccctt gcagcgtcca gatgttatcg ccgtccaccc ccgcgtcggg cgtcttcacc
    15721 cactttacgt atccaccaag caccaggatg ttctgcccgg cgccgccgtg cgacggcgaa
    15781 ttcgattgcg cccggtcccg aaacgcgaac ttgcccagaa acgatgtgct ctcgccgaaa
    15841 accgggttct ggtcggacat caccggcaag tccgggttgt cctctgacga cgggcgccgc
    15901 ccggccatgt tgaagtacgt gtagctcagg ttggcgtcat cgaggaagtc gttgcagcga
    15961 tgcacctggt cgatgggcat cggggcgccg ccgttcgagg ggcataaaaa caagcgcggg
    16021 tgagcgatga aatctttctg cagcaggggg tacagatgcc gccgattggg caccagcatt
    16081 acgccgggat cctggctcgg ggcccaggaa gcccgcgaat tccagcccgt aaacggcaga
    16141 ctggaaccat acgtcgccgc gtactgctga acgcccatcc ccagagccgc cagattggcc
    16201 gaacaggcca tccgctgccc acgctcgcgc aggtgcagca gactcggtgc cgcaacgcca
    16261 accatcagca cgatcatcgc cgccgcggcc acgatctcgc gcaggttccc taggcgcaca
    16321 attaccggcg cgccccgttc gacggtccgc gtaaggtcgt cggccgggcg aatcaggcgg
    16381 accggtcgcc ttgcggccgc gacccgcgcc gcaacccgcg tcgccaggtc gggcggggcc
    16441 gggtccgctt tgtactcgtg aagcgctttg aatgccgccg tcagggtctg gtgctgctgc
    16501 tgaagcccgg catcagcggc catccgcagg tttagctcga cttgttcgct cgcactcagc
    16561 cgcccgaggt gcagatccaa gagctgctcg tcgaatgtgg gtcccggttg catactgaac
    16621 tcagctcact tgctcatcgc tggcgttccg gccgcatctg cttgtgccac atccaggcga
    16681 accgcgccac cgcggcgtgc aacctggatt tcacggttcc gaccggaatg tccaaaatct
    16741 ccgcaatatc cgaatacggc aacctttgaa aatatccgag catcaggatg agctgcaggt
    16801 gctcgggcat tttcgctatc acgctgcgga cggcggcacg cacttcttcg gcactgctct
    16861 gctcgaagct cgactcctca gcggccggaa ccaactcgtc tgggcccagc ccgtcgtcgt
    16921 cggggccgcg ggcatccagc gattgttcca gccttcggcc gcgccctcgc aggtaatcgc
    16981 gtgccttgtt ggccgcgatc gtgtacagcc aagggcgaaa ggcccgctgc gggtcgaagg
    17041 cgtccgctgc caggtggacc tggaggaagg attcctgcac gaggtcatcc gcggcggccg
    17101 catttccgac aaagcgatgc aggaaagcga acaactcgtc ggcatagcgc tggacaagaa
    17161 tgtcgaaagc gccttttttg ccggctagat ggtccgccag caactcctca tcgcgtcggg
    17221 cgtccacagc ttccaacctc aggtacggca atgcgagcgg cgagttcgcc gcaagccggc
    17281 gaatgggcgg tgaaaagcgt taaagcctgc ccctgccgct acttatcggg ctcgctcacg
    17341 attcgaggat aacagaaacc gcaatttttt tcgcccccgc gcaggcacaa tcgccgcctt
    17401 attcgagagc tgcccgaaca cccgcccatg cggcggcgcc atgcccaagc acgcgtgcca
    17461 tgcccaagcc gcgtagcggc gcgggcatgc cgccgccagt cgctccacag ttgccacacc
    17521 gcccgtttgt gccgcaaccc acggcgcatt gaccggctca gacgtcacgt cagttccgcg
    17581 gcagaggccc gctgcgcgag gcgcgttctg gccaagtcga tagccagacg aatcgcggcc
    17641 cgcatgctgc ccgggttggc ccggtttcga ccggcaatat cgaaggccgt gccgtgatcg
    17701 gggctggtgc gcactgcccc caggcccaac gtgagctgca cggcgcggtg gaaggccagc
    17761 gccttgagcg ggatgagacc ctgatcgtga tacatcgcca ccaggccgtc gaattgcccg
    17821 gccacttcgg gcgtgaaggc ggtgtccggc ggtaggggcc cggtgacgtc tatttgcgcg
    17881 ctgcgcgcca ttgccagggc aggctcgatg atgcgctgct cctcatcgcc gagcatcccg
    17941 ttttcgccgg cgtgcggatt cagccccagc acgccgatgc gcgggcgcgg gattccgaac
    18001 cactcgcgca aagcccggtc cagaagatcg atcggctggt gcaccaggcc gatggtgaag
    18061 cggttgcgca actcgaacaa cggcacgtgc gcgctcgcca gcgccacccg cagctcgtcg
    18121 gcgacgaagg ccatagtgac gcgccgcgtc tcgaacatgt ccgacagcag gtgcgtgtga
    18181 ccgaccgcac gatgcccggc cagtttccag gcctctttgg aaatcggggc ggtaaccagt
    18241 gcgtctatca gcccggcttt caggctgcgg atgcctgcct cgacaaagcg gtaggaagct
    18301 tcgcccgcca gagcctcggg tccgcgcgtg ccgttgacgc gcaccgccgg cagctcgtcg
    18361 aaatcagcga ccagcacgcc gctaccgacg cgggcgcccg cttcgaacgg ctcgcgccac
    18421 cagaaggggt tgatttccgc cgcatcagcg gcgaccgcca gcacatcgtg caggccg
//
LOCUS       DAOSYS010000043        17998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2585_length_17998_cov_5.142284, whole genome shotgun sequence.
ACCESSION   DAOSYS010000043 DAOSYS010000000
VERSION     DAOSYS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17998
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_17998_cov_5.142284"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..2639)
                     /locus_tag="PLU99_04235"
     CDS             complement(435..2639)
                     /locus_tag="PLU99_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO16298.1"
                     /translation="MTRDEVCSKFLESLPYTLYPFQEEALLAWFASEAGVLIAAPTGM
                     GKTLIAEAAVFEALHTGQRLYYTTPLIALTDQKFRDFQELAERWGFRRDDVGLITGNR
                     RVNPEAPVRVVVAEILLNHLIAEDPAIADMEHVGAVVMDEFHSFNDFERGVVWELSLV
                     LLPKHVRVMLLSATVGNPYDFAYWLEKKHGRKLDVVITQERRVPLEYHWVEDKLLHEH
                     LVQMVSSNDSENRTPALVFAFNRDDCWELAEKLKGLALVSNKQKQEIEAVLAERQADL
                     AEGVGPKLKQMLLRGVGVHHAGVLPRHKEIVEDLFERKLVPFVCCTETLAAGINLPAR
                     SVVLVTLLKGKHGEKKLIPASSAHQMFGRAGRPQFDKQGYVYAVAHDDDVKINKWRKK
                     YDQIPAQTKDPGLLRVKKDLERKRPTRRKSEQYWSEGQFKTLIAAGPASLSSRSMIPY
                     QVLLYLLTRTGTLHETRDFLSKRFNSPDRIEKFQAQLNFMLDNLQALGYLKRSEDGDH
                     VTLDESIRKLLNFRSVDPLYGAFAAEMLNFSNFTEKVLVLESVLPLPPPVLRKVPIPE
                     DLLKGPLQSQVLEPQLIAMGVKLTGPEPDEDAPPEKPRWMRASWELNDEEERPPTFPE
                     MLPIIFQTRLAAPEPVMVEPKWVAGGTFELDCEFFKFVRSRDLIKQEGLILRHLLRLV
                     ILAGEFQMLTQDPEYEEIAEQTTRCCRRVDERYTERFLAEAESMRASALAKG"
     gene            2910..3296
                     /locus_tag="PLU99_04240"
     CDS             2910..3296
                     /locus_tag="PLU99_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO16299.1"
                     /translation="MSAFTPGQEICMALVAYLRQQGKTLRQIGEMVGLSESFVSRVAK
                     GERSFTLDHLALYERRLGEPLSVLIMESVWARDIDAEHKAAYDEALRVLRELGEFRRR
                     LAAGSVAKERRSARGTRSSGKTRRAG"
     gene            complement(3486..5009)
                     /locus_tag="PLU99_04245"
     CDS             complement(3486..5009)
                     /locus_tag="PLU99_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16300.1"
                     /translation="MALESLSSLALAAYIVVLSLVCIYGAHRYFLVGLYYRFRKAGRS
                     CASRFTDLPWVTVQLPMFNERYVAQRIIEQACRIDYPADRLEIQVLDDSTDDTQTIAR
                     KTVERMRAAGHNVVYVHRDNRTGYKAGALEAGLKTARGEFVAIFDADFLPPERILQDT
                     IHYFTDPAIGMVQTRWAHLNRDVSVLTQAQAVLLDGHFMIEHTARNRSGRYMSFNGTG
                     GVWRKAAITDAGGWQHDTLTEDLDLSYRAQLRGWRFVFLPDLTSPAELPPEMTAFKAQ
                     QHRWTKGGAQTCLKLLPRVLRSPAEWWIKLEAFFHLTSCVVYILIVLLSLLLGPALYA
                     KVIIGKDPPWLIRYESVLFVIGFGAPLLFYILSQRALQRGWFSSIKYIPALMAVGAGI
                     AFNNAIATLEGFFGKTGEFVRTPKFGEEARKTGDWRHRLAGFRHRRSWQVWAEFGIAL
                     YLIACLVGLFFFDNWYERVSAAIPFLLIFIGGYLYVALETFYTQWHASRHAAQSSPS"
     gene            5172..6233
                     /locus_tag="PLU99_04250"
     CDS             5172..6233
                     /locus_tag="PLU99_04250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOO16301.1"
                     /translation="MPKEIQDQTDALRRSRREADIERFIENQLPGPNQDLFKDMLITL
                     CRLARDGAGRGDLKLLNKALAELRYALRVFAPYSEIRKISIFGSSRTPEDDPNYQAAV
                     EFARQISESNWMIITGAGDGIMKAGHHGAGRAASFGVAIRLPFEQKTNTVIAADDKLV
                     NFRYFFTRKLVFIKEASAVALFPGGFGTLDEGFETLTLVQTGKAPIVPVVLVERPGGT
                     YWLRWRDYVEAELLRAGMICPEDMDLFTITDDVGLAVRVVRDFYRVYHSMRYVGSDLV
                     LRLERPLLPSTLELLNDTFGDVLARGRFEQTGALPAEDGELAEKPRLKFAFNRKSFGR
                     LRRLIDVINADPGALPPNP"
     gene            6948..7280
                     /locus_tag="PLU99_04255"
     CDS             6948..7280
                     /locus_tag="PLU99_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOO16302.1"
                     /translation="MAKAAKKVGAGKPRSKSEIFNELAEKAGITRKQVAALFGGLSEM
                     IKKDLTRGPKTFAVPGLMRINVIVKPATKARKGINPFTGQEMMFKAKPARKVVKIRPL
                     KGLKDMVV"
     gene            complement(7411..8175)
                     /locus_tag="PLU99_04260"
     CDS             complement(7411..8175)
                     /locus_tag="PLU99_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16303.1"
                     /translation="MTLDLQEVIADWNAEPGEISARLIVGRDGGELLQMFPEGRPDGK
                     RHHGLPTVLDYVEHEARLGRVQVAEEDARELQRELYQMNYRRLASSSLVEDCLMHKDV
                     DNARRHVERALRDIDGCLRRIDVIERCGRSGGVVLRPTLTFHRGRLRVQRAILDDRYE
                     DAIDEALRGRCALEEVLEEMGYEEEQRRSDPGVVFLHALAQRLRREYGIDLTLTERLN
                     AAVAEEDYALAAELREELKRRAATRPKARPENETNP"
     gene            complement(8377..9864)
                     /locus_tag="PLU99_04265"
     CDS             complement(8377..9864)
                     /locus_tag="PLU99_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOO16304.1"
                     /translation="MSLTCHLFGLLAQAAPATQEVTEAAVGNDLRSLGITLAYLLAAA
                     LFIFGLRMLSHPRTARQGNWLSSVGMLIAIVVTLADREIVSYTTIIAGLVVGSAIGWI
                     LAVRVQMTAMPQMVGMLNGFGGLASGLVALAEYIRVYIDPNRDPRLDVLIAIGLSALI
                     GMVTFTGSMTAFGKLQGWKIRGKPLSNPLLLPHHHPINIAQVLIAIVLIAVLVSNYEA
                     AWAIVLLYLLAAVLGVMLVTPIGGADMPVVISLMNSYSGLAAMTTGFVLDNYALIISG
                     SLVGASGIILTKIMCSAMNRSLLNVIAGGFGQELTAAGETSGGERTVRKMDAEEAAMV
                     LESAQKVFIIPGYGMAVAQAQHAVAELGDILRKKGVDVRYGVHPVAGRMPGHMNVLLA
                     EANVPYEMLSDLELNSEMEQCDVALVIGANDVVNPDARRPGSQIAGMPIFDVDKARTV
                     MVCKRSLNPGFAGIENELFFNQNTVMLFGDAKKMVTGIVSELKGK"
     gene            complement(9875..10156)
                     /locus_tag="PLU99_04270"
     CDS             complement(9875..10156)
                     /locus_tag="PLU99_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOO16305.1"
                     /translation="MIESFTNNFVVFVLAMFIGFEVIAKVPAILHTPLMSGANAISGI
                     TIVGALVIAGLHQGVWSEILGFLAVTFAMINVVGGYRVTDRMLAMFKRK"
     gene            complement(10153..11310)
                     /locus_tag="PLU99_04275"
     CDS             complement(10153..11310)
                     /locus_tag="PLU99_04275"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOO16306.1"
                     /translation="MKIAVPKEVRAGEQRVALVPESVKKLVKAGAQVAVESGAGSGAF
                     FSDAAYSEAGATIAVDAPNVYAEADFVLKVQPPGRLPDGRSEVELLRPGSLLLCTLNP
                     TRNLDVVQKLAAAKVTAFSTDAIPRTTRAQTMDTLSSMANIAGYKAVLIAAVELPKYL
                     PMFMTAAGTIFAAKVFVIGAGVAGLQAIATAKRLGAIVTATDVRRAVAEQIQSLGAKF
                     VGVEGGEDAEAAGGYARELTPEYQRQHAQVIARTCAASDVVITTALIGGVKAPKLITA
                     EMVAGMQPGSVIVDVAAEAGGNCELTKPGETVIQNGVKIVGPLNLPSSMPRDASVLYS
                     RNLTAFVLEFWKDGKFDLVLTDEIIAGALITHAGEVVHGPTKDAIASEDRQ"
     gene            11861..13132
                     /gene="odhB"
                     /locus_tag="PLU99_04280"
     CDS             11861..13132
                     /gene="odhB"
                     /locus_tag="PLU99_04280"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOO16307.1"
                     /translation="MAVELKIPEAGESITAVQIGRWLKSEGDFVQQDEPLVELESDKA
                     SMDLPAPVSGRISKLLKKTGDGARVGETIAVIDETAVAPDKPAAEKPAQPSEARAAVA
                     ATPRARPAHVMPAARRVLVERGLQAAEVDPTGPGGRLLKEDVQRHVATAPEQAAAVEA
                     RAGETPARAAPPPAFESSAPAARQEEAVPMTLIRRRIAERLVESQRNAAILTTFNECD
                     MSAVLDVRQRHGEAFEKKYGIRLGLMSFFVKAAIEALKLFPAVNAEIRDLPMSDGTTA
                     PHIVYKNYYDIGVAVSTDRGLVVPVIRNAERLSFAEVEKAIADLATRARDRKIAVEEL
                     QGGTFTISNGGVFGSLLSTPIINPPQSAILGLHVIQDRPVARDGQVVICPMMYLALTY
                     DHRIIDGRESVSFLRRIKECVEDPRRILVEI"
     gene            complement(13202..15103)
                     /locus_tag="PLU99_04285"
     CDS             complement(13202..15103)
                     /locus_tag="PLU99_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO16308.1"
                     /translation="MAVRRSRQRDVRRPKPAARGGGRGRTWRSIGLICAVLLAIGGTA
                     VAYYVRTTGGAEGLRPGAAAGFNLLLVTLDTVRADHLGCYGYAAARTPNLDALAASGL
                     RFAQAIAPAPLTTPSHATMMTGLVPPSHGVRDNGCRLADEQTTLAEILSARGYATAAF
                     ISSFTLESRYGLAQGFATYDDACAPPDRVVGHFGDTMANERSATAVTDAALNWLNDGS
                     RKNPFFAWIHYYDPHFPYAPPRAFAGDGQSRYDGEIAYVDSQLGRVRELLRQKKLQRR
                     TLIVVAADHGEGLEEHGEPTHAYLIYDSTLHVPLILNSPALFGAPRVIDDRVVSLADI
                     MPTVLDLLGVAVPAGLDGQHLLASKPDEDRALYIETMASRLNNGWASLHGLRRLHDKY
                     ILAPRPEYFDLKADPHELRNLVADAPDEVGELEARLAETMSRWESPEQVAAQTQGLSP
                     EEVQRLASLGYVGSPGEGVRVGVCDPKDMLPLWKRVMGAEIKSLQGRHAEAIAEIELV
                     VQQDPTDGHAWYYASYIYKRCGRMAEAEQALRQALTYSPNAAGYISLAQLLLLRRSLG
                     PEFERALNRARQLEPENGGIDIARGDWHVLQGRRDEALAAFERALRVDPIYSGKSARE
                     KMEWVRKNL"
     gene            complement(15397..15834)
                     /locus_tag="PLU99_04290"
     CDS             complement(15397..15834)
                     /locus_tag="PLU99_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HOO16309.1"
                     /translation="MPQSPVAAFETTMNKTHEWLKEVADAGGFTGEPQAYSALRAVLH
                     AIRDRLTVDEAAHLGAQLPMLVRGFYFEGWKPAASPHRERTWDEFVQNVRESLRGNPM
                     IEPERAIEATFRVLDQRITIGEINDIKRMLPQEVREHWIYAHA"
     gene            16217..16963
                     /locus_tag="PLU99_04295"
     CDS             16217..16963
                     /locus_tag="PLU99_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO16310.1"
                     /translation="MASWYDRLVGDEGSDYHRHVILPAALRLLDPQPGEQLLDLCCGQ
                     GVLVRALLAANPKLVVGVDASPKLIAAAKARGGADQRVRFVVRDARRLGDLAAAQFDA
                     AACVMAVHDLEAIDELFAQMSRALRPGGRAVVILLHPCFRIPRQSDWGWDETKKTRYR
                     RIDRYASPLSIPIATHPGRDPQQHTLFYHRPLADYINALGRAGLAIIACEELLTHRRA
                     EPGSHSRAENRAAQEFPLFVALKAIKGASS"
     gene            16973..>17998
                     /locus_tag="PLU99_04300"
     CDS             16973..>17998
                     /locus_tag="PLU99_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOO16311.1"
                     /translation="MRWAFRLSAAYNDAVLTIGPLQFDLPVVQAALAGYSDAPMRRIA
                     RRLGAPYALREVVLDKIVIQPGKRQRETLALSPDDHPIGGQLMGSQPEQIAEAAEIMA
                     RSGFDVIDLNFACPVKKVLGRCRGGYLLSDPQTAIDVLRRVRDALPAAVPLTLKLRRG
                     MDDSAESECNFFQILDAALEMNVAAVTVHPRTVRQRYVGPSDWSFLARVKRYAGQRTI
                     IGSGDLFTAEDIAAMFAQTGVDGVSIARGCIGNPWIFNAARALLRGDPAPPPPSVAEQ
                     GRVIREHYALATAEYGERRAPAVMRKFGIKYAELHPFYREVRDDFVRSGDERSWSAIL
                     DHWYDPTR"
BASE COUNT         3335 a         5814 c         5605 g         3244 t
ORIGIN      
        1 cgtgggttct cggcgttcgc cggcgttaat gccgtcaggc cgacacacaa agacagcaaa
       61 ccggcaacct tcatcgcact cgcaaacaac attctgtgcc ttccttcggc gcttggccga
      121 tctgtaatgc agcgtgcggg agcgtccaac gatcggcgcc gcccggcgac gactgcttag
      181 ccattaacat tcttctacgt tgccgcagtg aattgcgact aaattggggg tcaatatcga
      241 gttagtgcat ggaatggcgg gcgactaacc gactgtttaa aaaggccggg gccaggaagg
      301 gtgcgggggc cacaactaac aactaactac taacaactaa taatgcgtgg gaacgtggga
      361 aagtgggaaa gtgggaaagt gggaaagtgc gaacatgaga aaagtacttc aaactcgaga
      421 cttgaaacct tctgtcagcc cttcgccagc gccgaggcgc gcatgctctc cgcctccgcc
      481 aggaagcgct cggtatagcg ttcatcaacg cggcggcagc aacgcgtggt ctgctcggca
      541 atctcttcgt attcggggtc ttgcgtcagc atctggaatt cccccgcaag aatcactagc
      601 cgcaatagat gacgcaggat caagccctcc tgcttgatca ggtcgcggct gcgaacgaat
      661 ttgaagaact cgcaatcgag ctcgaacgtc ccgccggcca cccacttggg ctcaaccatt
      721 accggctccg gagccgccag gcgcgtctgg aagatgatcg gcagcatttc cgggaacgtc
      781 ggcggacgct cttcctcgtc gttaagttcc cacgaggccc gcatccagcg cggcttctcg
      841 ggcggcgcgt cctcgtcggg ctccggcccg gtcagcttca cgcccatcgc gataagctgc
      901 ggctccagca cttgggattg cagcggcccc ttcagcaggt cctcggggat cggcaccttc
      961 cgcagcaccg gcggcggcag cggcaggact gactccagca ccagcacctt ctcggtaaag
     1021 ttgctgaagt tcagcatctc ggccgcgaac gccccgtata gcgggtcgac gctgcggaag
     1081 ttcagcagct tgcggatgct ctcgtcgagc gtcacgtgat cgccgtcttc ggagcgcttc
     1141 aggtagccca gcgcctgcag gttgtcgagc atgaagttga gctgcgcctg gaacttctcg
     1201 atgcggtcag gtgagttgaa acgcttgctc aggaaatcgc gcgtctcgtg cagcgtgccg
     1261 gtgcgcgtca gcaggtacag cagcacctgg tatggaatca tcgaccggct cgacaagctg
     1321 gccggtccgg ccgctatcag cgtcttaaac tgcccttcgc tccagtactg ctcggacttg
     1381 cggcgcgtcg ggcgtttgcg ctccaagtcc tttttgaccc gcagcaggcc ggggtccttg
     1441 gtctgggccg gaatctggtc gtacttcttg cgccacttgt tgatcttcac gtcgtcgtcg
     1501 tgcgcgacgg cgtaaacgta gccttgcttg tcgaactgcg gccggccggc gcgcccgaac
     1561 atctggtggg cgctgctggc tgggatgagt tttttctcgc catgcttgcc cttgagcagc
     1621 gtgacgagga cgaccgagcg ggccggcagg ttgatgccag cggccagtgt ttcggtgcag
     1681 cagacgaatg gcaccagctt gcgttcgaag aggtcttcga cgatctcttt atggcgcggc
     1741 agcacgcccg cgtggtgcac gccgacgccg cgcaggagca tctgcttcag cttcggcccg
     1801 acgccctcgg ccaggtcagc ctgccgctcg gcgagaacgg cctcaatctc ctgcttctgc
     1861 ttgtttgaga ccagcgccag ccctttgagc ttttcggcca gctcccagca atcgtcgcgg
     1921 ttgaaggcga atacgagggc cggcgtgcgg ttttccgagt cgttcgacga gaccatctgc
     1981 accaggtgtt cgtgcagcag cttgtcctcg acccagtgat attcgagcgg tacgcgccgc
     2041 tcctgcgtga tcaccacgtc gagtttgcgg ccgtgcttct tttccagcca ataggcgaag
     2101 tcgtaggggt ttccgacggt ggccgacagc agcatgacgc gcacgtgctt gggcagcagc
     2161 acaagcgaca gctcccagac gacgccgcgc tcgaagtcgt tgaagctgtg gaactcgtcc
     2221 attacgacgg cgccgacgtg ttccatgtcc gcgatggccg ggtcttcggc gattaagtgg
     2281 ttgagcaaga tttcggccac gacgacgcgc accggggcct ccgggttcac gcggcgattg
     2341 ccggtgatca ggccgacgtc gtcgcggcga aagccccagc gctcggccaa ttcctggaaa
     2401 tcgcggaact tctggtcagt gagcgcgata agcggcgtgg tgtaatacag ccgctggccg
     2461 gtgtgcaggg cctcgaacac ggccgcctcg gcgatgagcg tcttgcccat cccggtcggc
     2521 gcggcgatca gcacgccggc ctcggaggcg aaccaggcca gcagggcctc ttcctggaac
     2581 ggatagaggg tgtacgggag ggattcgagg aacttggagc aaacctcgtc gcgcgtcatg
     2641 ggcgcaaggt acagacgatg aatgcaaaac gcaaaacgag gccttcgtgc gccggcaagg
     2701 ctgccgggcg gcggcgcccc cggggtgact ccagggggcc gttttgattt gccggcttaa
     2761 cttgcctatt gggcaagtcg ctcgtatact cagggtagac ccgcggcggt actcgcgcga
     2821 tcaggaagct tcgctctcaa gcagtggcac gcagtgaggc gcgccgtgtg agtcccgcgt
     2881 gtcaggctac aacgaaaggc ggcgaactcg tgagtgcttt cacgccggga caggaaattt
     2941 gcatggccct ggtggcctac ctgcggcagc agggcaagac gctgcggcaa atcggagaaa
     3001 tggtggggct gagcgagtcg tttgtaagcc gggtggccaa aggcgagcgc agttttacgc
     3061 tcgaccatct tgcgctttat gagcgtcgct tgggcgagcc gctgtcggtg ctgatcatgg
     3121 agtcagtatg ggcgcgggat atcgatgccg aacacaaagc ggcctatgac gaagcgttac
     3181 gcgtgctgcg cgaattgggc gagtttcgac ggcggctggc cgccgggagt gtagccaaag
     3241 agcggcggtc ggctcgggga actcggtcaa gcggcaagac gcggcgggct gggtaaggca
     3301 aatccggtag cgacgcgaat ggcaacagag tgataaagtg atagagtgat aaagtgataa
     3361 gtgattcgcg gatgcccctt atcaccctat caccttatca cgcccacaca ccgcccccgg
     3421 cactgcttgg agctgcgctt tcaagcagtg gcacacactg cgttttcaag cagcggcaca
     3481 caccctcatg acggcgaaga ttgcgccgcg tgccggctgg cgtgccattg cgtgtagaac
     3541 gtctccagcg cgacgtagag gtagccgccg atgaagatga gcaggaacgg gatggcggcc
     3601 gagacgcgct cgtaccagtt gtcgaagaag aataggccga ccaagcacgc gatcaagtag
     3661 agcgcgattc cgaattcggc ccagacctgc catgagcggc ggtggcgaaa accggccaag
     3721 cggtgccgcc aatccccggt ctttcgtgcc tcttcgccga acttgggcgt gcgaacgaac
     3781 tcaccggtct tgccgaaaaa accctcgagt gtggcgatgg cgttgttgaa tgcgattccc
     3841 gccccgacgg ccatcagtgc cgggatgtac ttaatgctgc taaaccatcc gcgctgcagg
     3901 gcgcgttggc tcaggatgta gaacagcagt ggtgcgccga acccgatgac gaacagcacc
     3961 gattcgtacc tgatcagcca aggcggatct ttgccgatga tcaccttggc ataaagcgcc
     4021 gggcccagca gcaagctcag cagaacaatc aaaatgtaca cgacgcagct cgtgaggtgg
     4081 aagaacgctt caagcttgat ccaccactcc gcgggactcc ttagaacgcg cggcagcagc
     4141 ttcaggcacg tctgcgcgcc gcccttggtc cagcggtgct gctgagcctt gaacgccgtc
     4201 atctcgggcg gcagctcggc cggagacgtc aaatccggca ggaagacgaa ccgccagccg
     4261 cgcagttgag cccgatagga aaggtcgaga tcctccgtta aggtgtcgtg ctgccagccg
     4321 ccggcgtcgg taatggcggc tttccgccac accccgccgg tgccattgaa gctcatatag
     4381 cgcccggaac ggtttcgggc ggtgtgctcg atcatgaagt gcccgtcgag gagtacggcc
     4441 tgggcctgag tcaggaccga gacatcccgg ttcagatggg cccagcgggt ctggaccatg
     4501 ccgatcgccg ggtcagtgaa gtagtgaatc gtgtcctgga gtatccgctc tggcggcaga
     4561 aagtccgcgt cgaagatggc gacgaactcg ccgcgggcgg ttttcaggcc ggcctcgagg
     4621 gcgccggcct tgtagccggt gcggttgtca cggtgaacgt agacgacgtt gtgcccggcg
     4681 gcccgcatgc gctcgacggt tttgcgggcg atggtctgcg tgtcgtcggt ggaatcatcc
     4741 agaacctgga tttcgagccg gtcggcaggg taatcgatac ggcaagcctg ctcgatgatc
     4801 cgctgggcga cgtagcgctc gttgaacatc gggagctgca cggttaccca gggcaggtcg
     4861 gtaaaacggc tggcgcagga ccggccggcc ttgcggaacc gataatacag gccgacgaga
     4921 aagtagcggt gagccccgta gatgcatacg aggcttaaca cgacgatgta agcggccagg
     4981 gcgagtgaac ttaaggattc aagtgccatg ttgcttcggt cccaccgcgc cgggacgata
     5041 cgcgcaccat tgcgggtgac tatcgtagaa taaaccgtcc ggcggtgtca atcatgcgag
     5101 aaccgattcc agcctccgag tgtcataaag agacatccgg catcgacagc caacttgcct
     5161 caggagcacg cgtgcccaaa gagattcagg atcaaaccga cgccctgcgc cgcagccggc
     5221 gcgaggccga catcgagcgt ttcatcgaaa atcagttgcc tggcccgaac caggatctat
     5281 tcaaggatat gctgataact ctttgccggc ttgcgcgcga cggcgccggg cggggggatt
     5341 tgaagctgct gaacaaagcc ttggcggagt tgcgctacgc gcttcgcgtt ttcgccccgt
     5401 attcggagat tcgcaagatc agcatcttcg gttcgtcccg gaccccggaa gacgacccta
     5461 actaccaggc cgcggtggag tttgcgcggc agattagcga gagcaactgg atgattatta
     5521 cgggggccgg cgacgggatc atgaaggccg ggcaccacgg cgcgggacgg gcggccagtt
     5581 tcggggtggc gatccggctg ccgttcgagc agaagaccaa cacggtgatc gccgcggatg
     5641 ataaactggt caacttccga tatttcttca cgcgcaagct ggtgttcatc aaggaggctt
     5701 cggcggtggc gctcttcccg ggcggcttcg gaacgctcga tgaggggttc gaaacgctga
     5761 cgctggtgca aaccggcaag gcgcccatcg tccccgtagt gctcgtggag cgccccggcg
     5821 gtacgtactg gctgcgctgg cgcgactacg tggaagcgga actgctgcgg gccgggatga
     5881 tctgccccga ggacatggac ctgttcacga ttacggacga cgtcgggctg gcagtgcgcg
     5941 tagttcgcga cttctaccgc gtttaccact ccatgcgcta cgtcggcagc gacctcgtct
     6001 tgcggctcga gcggcctcta ctgcccagca cgctggaact gctgaacgac actttcggcg
     6061 acgtcctggc acgcggacgc ttcgagcaga ccggggcgtt gccggccgaa gatggtgaat
     6121 tggccgaaaa accgcggctg aagtttgcgt tcaatcgcaa gagcttcggg cgtctgcggc
     6181 ggctgattga cgtgatcaat gcggacccgg gcgccctgcc ccccaacccc tgatcggcgt
     6241 ctgcaaggct agacgtccag agcccgcggg cgggcgaccg ttggcagcgt ctccctggta
     6301 gcctttctga ctgtgtgcgc ctggccgtct ttgacgatgg caatgggccc gcgctctact
     6361 atgagagctc ggggccgtgc ctcgcaaaga gggacgggtc cgcctggtcg gacgtcgggc
     6421 gcggcgtaac cggtttcgac tacctcactg ggcgagggtc tggggcgcga catggacact
     6481 tggaagatct gcacctgagg tgttatctcg aggcggcgga ttgcaatgcc gacggcgcgg
     6541 tgaacgtctt cgatagtgac ccgtttgtgg aggcgctcct gggcaggtag cggtcaagcg
     6601 cgcccggggt gaaggcccag cccgagcacc gtagaccgtg ttcttgacca accgttcgtc
     6661 ttgctagggc ggagcgattg ccggcgcgga gcggtttttg gcggccagcg ctggccgaac
     6721 gacgcctaaa gtcgcaaaaa gcccgagttt tctgcatgtt ttgcacaaga agcccttgac
     6781 ggaacgcgaa tttcgtgcaa aaagttgtgt acttccgtgg cgcggtcaaa aacccagctt
     6841 gagcgcaggc agtggcgcct gcgaacggaa tgggaaatgc gcgttcgtaa cagcagtaac
     6901 gggcccggcg ccgcaagcgc cgcaggagcc cgggaggacg agcaaggatg gctaaggcag
     6961 cgaagaaagt tggggcagga aagccccgga gcaagtcgga gatcttcaac gaactcgccg
     7021 agaaggcggg catcacacgc aaacaggtcg cggccctgtt cggcggcctg agtgagatga
     7081 tcaagaagga tctgaccagg ggcccgaaga ctttcgcagt ccccggcctg atgagaatca
     7141 atgtcatcgt caagcccgcg accaaggccc gcaagggtat caatcccttc accggccagg
     7201 aaatgatgtt caaagccaag ccggcccgca aagtggtcaa gattcgcccg ctcaagggcc
     7261 tcaaggacat ggtcgtctag accgaatcgc ggttttccga ccgatcgcat accggcggcg
     7321 ccggccctaa caagccggcg ctgccggaac gtgcaagccg caagtcacca atcaatactg
     7381 agatgcgctt gcgtcgagaa tcgagcaggc tcaggggtta gtttcgtttt ctggtcgggc
     7441 tttggggcgg gtggcggcgc gccgcttcag ttcctcgcgc aattcggccg caagggcgta
     7501 atcctcctcc gccacggccg cgttcaggcg ttcggtaagc gttaagtcga tgccgtattc
     7561 gcggcgcagt cgctgtgcca gcgcgtgcag gaagacgacg cccgggtcgc tgcggcgctg
     7621 ttcttcctca taccccatct cctccagaac ctcctccaga gcgcaccgcc cgcgcaacgc
     7681 ctcatcaatt gcatcctcgt agcggtcgtc cagaatcgcg cgctgcaccc gtagtcgccc
     7741 gcggtggaag gtcagcgtgg ggcggagcac aacgccgccg gatcggccgc agcgctcaat
     7801 gacgtcaatg cgcctcaggc aaccgtcgat atcgcgcagc gcccgctcga cgtgccggcg
     7861 ggcattgtcg acgtctttgt gcatgagaca atcttcgacc aggctggacg aggccaggcg
     7921 gcggtagttc atctggtaaa gttcgcgctg cagctcgcgc gcatcttcct ccgcgacttg
     7981 cacgcgcccc agccgggctt cgtgctccac gtagtccagc acggtcggca ggccgtggtg
     8041 acgctttcca tccgggcgac cctcgggaaa catctgcaag agctcgccgc cgtcgcggcc
     8101 aacgatcagc cgcgcgctga tttcacccgg ttcggcattc cagtccgcga ttacttcctg
     8161 cagatcgagc gtcatgataa gcgccgctgg cgatgctggt gttatcgtct gggtgcaatc
     8221 gagactgcaa tcaggccttt tcaacggagc cgccaggccg gagcgtccta tcagcaccgg
     8281 cctcattcaa ggacccgttc gacacgaccg aaaaaagtgc ccgctgcggg ctgtgctcat
     8341 tccacagccg cccgttcagg cgcgccgtcg gcgccgctac ttgcccttca gctcactgac
     8401 gatgccggtc accatcttct tggcgtcgcc gaagagcatc acggtgttct ggttgaagaa
     8461 cagctcgttc tcaatcccgg caaaacccgg attcaagctg cgcttacaga ccatgaccgt
     8521 gcgggccttg tcaacgtcga agatcggcat gccggcgatc tggcttccgg ggcggcgggc
     8581 gtccgggttg accacgtcat tggccccgat caccaaggcc acgtcgcact gctccatctc
     8641 cgagttcagc tcgagatcgg agagcatttc gtacgggacg ttggcttcgg ccagcaggac
     8701 gttcatgtgc cccggcatgc gcccggccac cggatgcacc ccgtagcgca cgtccacgcc
     8761 ctttttgcgg agaatgtcgc ccaactcggc cacggcgtgc tgcgcctggg cgacagccat
     8821 gccatagcca ggaataatga agaccttctg cgccgactcc agcaccatgg ccgcttcttc
     8881 ggcgtccatc ttgcgcaccg tgcgctcgcc gccggatgtc tcgcccgccg cggttaactc
     8941 ctgcccgaat ccgccggcga tgacgttcag gagtgagcgg ttcatggcgc tgcacatgat
     9001 cttcgtcagg atgatgcccg acgcgcccac cagcgagccg ctgatgatca gggcgtagtt
     9061 atccagcacg aagccggtgg tcatcgccgc caggcccgaa tacgagttca tgagcgagat
     9121 aactacgggc atgtcggctc caccgatcgg cgtcaccagc atcacgccca gcaccgccgc
     9181 cagcaggtaa agaagaacta tcgcccaggc cgcttcatag ttggatacca gcaccgctat
     9241 gagaacgatc gcgatcagca cttgcgcgat attgatcggg tggtgatgcg gcagcagcag
     9301 cggattgctc aggggtttgc cacggatttt ccagccttgc agcttgccaa aggccgtcat
     9361 gctgccggtg aaagtcacca tgccgatcag cgcgctcagg ccgatcgcga tcaacacatc
     9421 gagccgcggg tcgcggttcg ggtcgatata gacgcggatg tactccgcca gcgccacaag
     9481 ccccgaagcc agcccgccga agccgttcaa catcccgacc atctgcggca tggcggtcat
     9541 ctgcacccgc acggccagaa tccacccgat ggccgaaccc accaccaggc cggcgatgat
     9601 ggtcgtgtag ctgacgattt cgcggtcggc cagcgtcacc acgatggcga tcagcatacc
     9661 cactgaggag agccaattgc cctgccgcgc ggtgcgcggg tgcgacagca tgcgcaaccc
     9721 gaagataaac agggcggccg ccagcagata tgcgagggtg atgcccaggg agcgcaggtc
     9781 gttccccacg gccgcttccg taacttcctg cgtggcgggc gcggcctggg cgagcaggcc
     9841 aaacagatga caagtcaaac tcatggccgg cctcctactt tctcttgaac atggccagca
     9901 tgcggtcagt gacgcgatag ccgccgacca cgttgatcat ggcgaacgtg accgctaaga
     9961 agccgaggat ttcgctccag accccctggt gcaaaccggc gatgaccaaa gcgccgacta
    10021 tcgtgatgcc ggagatggcg ttcgcgccgg acatcagcgg cgtgtgcaga atcgccggca
    10081 ccttggcgat gacttcaaag ccgatgaaca ttgccagtac gaagactacg aagttgtttg
    10141 taaagctctc gatcattggc gatcctcgct tgcgattgcg tctttggtcg gtccgtgcac
    10201 cacctcgccg gcgtgcgtta tcagtgcccc cgcgatgatc tcgtcggtaa gcaccaggtc
    10261 gaatttgccg tctttccaga actcgagcac gaaggctgtc aggttccggg aatacagcac
    10321 gctcgcatca cgcggcatgc tggagggcag attaagcggg ccgacgattt tgaccccgtt
    10381 ctgaatgacc gtctcgcccg gcttggtcaa ctcgcaattg ccgccggcct ccgcggcgac
    10441 gtccacgatt accgagccgg gctgcatgcc ggccaccatt tcggccgtga tcaacttggg
    10501 cgccttcaca ccgccgatca gcgcagtggt gatgaccacg tcgctggcgg cgcaggtgcg
    10561 ggcgatcacc tgggcgtgct gccgctggta ctcgggcgtc aactcgcgcg cgtagccgcc
    10621 ggccgcctcg gcgtcttccc cgccctcaac gccgacgaat ttcgccccca ggctctggat
    10681 ttgctccgca accgcgcggc gcacgtccgt ggccgtcacg atcgctccca gccgcttggc
    10741 ggtggcaatc gcctgcaagc cggccacgcc cgccccgatt acgaaaactt tggcggcgaa
    10801 aatcgtgccg gccgcggtca tgaacatcgg caggtacttg ggcagctcga ccgccgcgat
    10861 cagcaccgcc ttatacccgg cgatattcgc catcgaggac agcgtgtcca tggtttgcgc
    10921 ccgcgtcgtg cgtgggatcg cgtccgtcga gaaggccgtt actttcgcgg ccgccagctt
    10981 ctgcacgacg tcgagattcc gggtcggatt cagcgtgcac agcagtagag aaccggggcg
    11041 cagcagttct acctcgctgc gcccgtcagg caggcggcct gggggctgga ccttcagcac
    11101 gaagtcggcc tcggcgtaaa cgttaggggc gtcgacggcg atcgtggccc cggcttcgga
    11161 gtatgcggcg tcgctgaaga acgccccgct ccccgccccg ctctcgaccg cgacctgcgc
    11221 gccggccttg accaactttt tgacggattc cgggaccagc gccacccgct gctccccggc
    11281 ccgaacctct tttggaacgg cgattttcat ggcagacctc gatggcagac ttcgcatgcg
    11341 ccagcgccgc gatgcaagca cgaccacggc ccgagagcgg tcggcccatc cggcggagtg
    11401 ggcagtatat cagggagcgt ttacgtcgaa agggccgcgg cagcagcgcg ccttggctac
    11461 gcgcaatcaa atccaatcgt ccctacggga acagagccgg ggcgtgtcgc ctcgaaaagc
    11521 cgccagggcc tcccgcccac attgtaattg ccgcgggagc gcttacggcg actgcccctg
    11581 tcgcccaaag gtatcccgcc gcccgaggac gcagaatccg acccgtgatg tcggtcaccg
    11641 cagtgcttct tcgcggagaa ttccacagtg cttttttcac ggagggattt gaaagtgctt
    11701 cttcaaggag gattcgaagc ggtgtcgact ccgccgaaca caaatgagca cacgaaaagc
    11761 gcactccaaa agcgcactag cttccttacg gagggcctct tccgtataac atgtgacgat
    11821 gttgcgttcc cagtgaacgg gtacacgagc aggggcaaag atggccgtcg agctgaaaat
    11881 ccccgaagcg ggcgagtcta ttactgcggt acagatcgga cgctggctta aatccgaggg
    11941 cgacttcgtc caacaggacg agccgctggt cgaactcgag agtgacaaag cctccatgga
    12001 tctgccggcg cctgtaagcg gccgcattag caagctattg aagaagaccg gcgacggagc
    12061 gcgcgtcggc gagaccatcg ccgtcatcga cgagaccgct gtcgcgccag acaagccggc
    12121 tgcggagaag cccgcccagc ccagcgaggc gcgcgcggcg gtcgccgcca cacctcgcgc
    12181 ccggccggcc cacgtcatgc ccgccgcacg ccgcgttctg gtcgaacgcg ggctgcaggc
    12241 tgctgaagtt gaccccactg gtcccggtgg tcgtttgctc aaggaggacg tgcagcggca
    12301 cgttgcgact gcgccggagc aggcggccgc ggtcgaggcg cgggccggcg aaacccccgc
    12361 gcgcgcggcg cctccgccgg cattcgaatc ttccgcgccc gccgcgcggc aggaagaagc
    12421 cgtccccatg acgctcatcc gccgccgcat cgccgagcgc ctggtcgagt cgcagcggaa
    12481 cgccgcgatt ctgacgacgt ttaacgaatg cgacatgtcg gccgtcctcg acgtgcgcca
    12541 gcggcacggt gaggcgttcg agaaaaagta cggcatccgc ctgggcctga tgtccttctt
    12601 cgtcaaggca gccattgagg ccctgaagct cttccccgcc gtcaacgccg aaatccgaga
    12661 cctgccgatg tccgatggca caacggcccc gcacatcgtc tacaagaact actacgacat
    12721 cggcgtggct gtcagcaccg accgcggcct ggtggtgccg gtgatccgca acgccgagcg
    12781 tctgagcttc gccgaagtgg aaaaagccat cgccgacctc gcgacgcgcg cccgcgaccg
    12841 caaaatcgcc gtcgaggagt tgcagggcgg cacattcacc atcagcaacg gcggcgtctt
    12901 tggctcgctg ctttcaacgc cgatcatcaa tccgccgcaa agcgccatcc tcggcctgca
    12961 cgtcattcag gatcgcccgg tagcgcgcga tgggcaggtg gtgatttgcc ccatgatgta
    13021 cctcgcgcta acctacgatc accgcatcat cgatggccgc gaatcggtct cgttcctgcg
    13081 gcgcatcaag gagtgcgtcg aggacccgcg ccgcatcttg gtggaaatat agattccgcg
    13141 gctcccttta cgccggtccg gccgccaaaa gggcctgccc cggtcgcgtg cagccgggcc
    13201 gtcacaggtt ctttcgaacc cactccatct tctctcgggc gctttttccc gaatagatcg
    13261 gatccacgcg cagcgcgcgc tcgaaggcgg ccaacgcctc gtcccgccgc ccttgcagta
    13321 cgtgccagtc accccgcgcg atgtcaatgc cgccgttctc cggctccagt tgccgcgccc
    13381 ggttgagcgc gcgctcgaac tccggtccca gactgcggcg gagaagcagc agttgcgcga
    13441 ggctgatgta acccgcagcg tttgggctgt aagtcaaggc ttgccgcagc gcctgctcgg
    13501 cctcggccat gcgcccgcaa cgcttataga tgtaagaagc gtagtaccag gcgtgcccgt
    13561 cagtcggatc ctgctgcact accagctcga tttccgcaat cgcctctgcg tgccgtccct
    13621 gcagcgactt gatctccgct cccatgacgc gcttccacag cggcagcatg tccttgggat
    13681 cacacacgcc gacccgcacg ccctcgcccg ggctgcccac gtaccccagc gacgccaggc
    13741 gctgcacctc ttcgggcgac agcccctgcg tctgggccgc aacctgctcc ggcgattccc
    13801 agcggctcat cgtctcagcc agccgcgcct ccagctcgcc cacctcgtcc ggtgcatccg
    13861 caaccaggtt ccgcaactcg tgcgggtcgg ccttcaggtc gaagtactcg ggccgcggcg
    13921 caagtatgta cttgtcatgc agccgccgca gcccatgcag cgaggcccag ccattgttca
    13981 gccgcgacgc catcgtctcg atatagaggg cgcggtcttc gtccggctta gacgccagca
    14041 ggtgctgccc atccagcccc gcggggaccg caacgccaag caaatccagc accgtgggca
    14101 tgatgtccgc caggctgacc acgcgatcgt caatcacgcg cggcgcgcca aacagcgccg
    14161 ggctgttgag gatcaaggga acgtgcagcg tcgagtcgta tatcaggtac gcgtgcgtcg
    14221 gctcaccatg ttcctccagc ccctcgccgt gatcggcggc caccacgatc agcgtccgcc
    14281 gttgcagctt cttctgccgc agcagctccc gcacccgccc aagctgcgag tccacatacg
    14341 cgatttcgcc gtcgtatcga ctctgcccgt ctccagcgaa tgcccgcggt ggggcataag
    14401 gaaagtgcgg atcataatag tgaatccacg caaagaacgg gttcttgcgc gacccatcgt
    14461 tcagccagtt cagcgccgca tccgtaacgg ccgtcgccga acgttcattg gccatcgtgt
    14521 cgccgaagtg accgaccacc cggtccggcg gcgcacaggc atcatcatac gtcgcaaagc
    14581 cttgcgccag gccataccgg ctttccagcg tgaaggagct gatgaacgcc gccgtggcat
    14641 agccccgcgc agagaggatt tccgccagcg tagtctgctc gtccgccagg cggcagccgt
    14701 tgtcccgcac gccgtgggag ggcggaacca ggccggtcat catggttgcg tgcgagggtg
    14761 tcgtcagcgg cgccggcgca atcgcctgcg caaaacgcaa ccccgaagcg gccagggcat
    14821 cgagattggg ggtgcgcgcc gccgcatatc cgtagcagcc aagatgatcg gcgcgcaccg
    14881 tgtccagcgt tacgagaagc agattgaatc ccgctgccgc tcccggcctc aatccctctg
    14941 ccccacccgt ggtgcggacg tagtaagcga ctgctgtgcc gccgattgcc agcagtacgg
    15001 cgcagatcag tcctatgctc cgccaagtgc ggcctctgcc cccgcccctc gccgccggct
    15061 tcgggcggcg aacgtcacgt tgcctgctgc ggcgtaccgc cattcaggtc actcctggaa
    15121 ctcaaaggtt caagcgaaac gcgggagcta tcgggcagcc ggtttcaccc tcgtgccttc
    15181 agggaccggc ctgatcccac gccgctgaac cggtttttgg gtggatggtc ttttttgctc
    15241 acgcgcggcg agcggccgtt gtgagcaatc ccccaggttg tgtgccctcg gttactgcca
    15301 tcctcctatg tacacggcta attgtaccaa gaaatccggt gaatcaataa catttcttaa
    15361 aaatcgcgtg caacgtccga tacgggcgtt gaagaatcat gcgtgggcat aaatccaatg
    15421 ctcgcggacc tcctgcggca gcatgcgctt gatgtcgttt atttcgccga tggtgatccg
    15481 ctgatccagt accctgaatg tcgcctcaat ggcacgctcc ggttcgatca tcggattgcc
    15541 gcgcaagctc tcgcgcacgt tctgcacgaa ctcgtcccag gtgcgctcgc ggtgcggcga
    15601 cgccgccggc ttccaccctt cgaaataaaa gccgcgcacc agcatcggca actgcgcccc
    15661 caaatgggcc gcctcatcca ccgtcaaacg gtcgcggatc gcgtgcagca cggcgcgcag
    15721 cgccgaataa gcctgcggct cgcccgtgaa accgcccgcg tcggccacct ccttcagcca
    15781 ctcgtgcgtt ttgttcatcg tcgtttcaaa tgcggccacc gggctctgcg gcatggtatt
    15841 ctcctttcac ggataagacc ggtccgaaga atcttctcaa aagagcctga acggcctctg
    15901 aattgagact gaccttacgt taagccactc taagattgcc gaatgattcc gattctgtga
    15961 gatttccgct tgcgagcgtg tagtatctgc cggtctaatc ggagctcgtt cggcagcgaa
    16021 ggcaaccgag gctgcggcct ccggtagctg ctggccgctt actaggtatt ggccgtctat
    16081 taggtaatca gtccgttcgc gaatgcaccg accggttcga aaaacagaac gcccggcgtc
    16141 tcgtcccgcg cgcaggcatg cgcgacagcc gcgcacggac gccggccgca gtgccccaac
    16201 gcgcggctgg gatcatgtgg cgagttggta tgaccggctg gtgggagacg agggctcgga
    16261 ttatcaccgc cacgtcattc tgccggcggc gctgcgtctg ctggatccgc agccgggcga
    16321 gcaattgctg gatttgtgct gcggacaggg cgtgctggtg cgcgccctgc ttgcggcgaa
    16381 ccccaagctg gtcgtcggag tggatgccag ccccaagctg atcgccgccg ccaaagctcg
    16441 cggcggggct gatcagcggg tgcgattcgt cgtgcgcgac gctcgccgcc tgggcgactt
    16501 ggcggccgcg cagttcgatg ccgcggcctg cgtgatggcg gttcacgacc tcgaggccat
    16561 cgatgagctg ttcgcccaaa tgtcgcgcgc tctgcgcccg ggcgggcgcg ccgtggtcat
    16621 tcttctgcac ccgtgcttcc gaattccgcg ccagtcagat tggggctggg acgagactaa
    16681 gaaaacccgt taccggcgca tcgaccgcta cgcgtccccg ctgagcatcc ccatcgccac
    16741 tcatcccggc cgcgacccgc agcagcatac gcttttttac caccggccgc tggccgatta
    16801 catcaacgcc ctcgggcggg ccggtctggc catcatcgct tgcgaagagc tgctcacgca
    16861 tcgccgtgcg gagcccggca gccacagccg cgccgaaaac cgggccgctc aggagtttcc
    16921 tctctttgtg gctctgaagg cgatcaaggg cgcgtccagt tgaacgccgt ctgtgcgatg
    16981 ggcattccgg ttaagcgccg cctacaatga cgccgtgcta accatcggcc cgctgcaatt
    17041 cgatctgccg gtagtccagg ccgcgctggc cggctatagc gacgccccca tgcgccgcat
    17101 cgcccgccgc ctgggcgccc cgtacgccct gcgcgaagtc gtgctggaca aaatcgttat
    17161 acaacccggc aagcggcagc gcgaaaccct ggcactgagc cccgacgacc accccatcgg
    17221 cggacagctc atgggttccc aacctgagca aatcgccgag gccgctgaaa tcatggcccg
    17281 cagcggcttc gacgtaatcg acctgaactt cgcctgcccg gtcaagaagg tgctcggccg
    17341 ctgccgtggc gggtacctgc tctcggaccc gcagaccgct atcgatgttc tacgtcgcgt
    17401 gcgggacgcc ctgccggccg cagtcccgct cacgctgaag ctccgccgcg gcatggacga
    17461 ctcggccgag agcgaatgca acttcttcca aattctggac gccgccctgg agatgaacgt
    17521 cgccgccgtt acggtgcacc cacgtaccgt gcggcagcgc tacgtcggcc cgagcgactg
    17581 gtcgtttctg gcgcgcgtca aacgttacgc gggccagcgt accatcatcg gcagcggcga
    17641 cctgttcact gccgaggaca tcgccgccat gttcgcccaa accggcgtcg acggtgtcag
    17701 catcgcgcgc ggctgcatcg gcaacccgtg gatattcaac gcagcgcgcg cccttctgcg
    17761 cggcgatccc gccccgccac cgcccagcgt cgccgagcag ggacgcgtca tccgcgaaca
    17821 ctacgccctg gccaccgccg aatacggcga gcggcgtgcc ccggccgtga tgcgaaaatt
    17881 cggcatcaag tatgccgagc tgcacccgtt ctatcgcgag gtccgcgacg acttcgtccg
    17941 cagcggcgat gaacgaagct ggtcggccat cctcgaccac tggtacgacc ccacgcgc
//
LOCUS       DAOSYS010000044        17908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2600_length_17908_cov_5.662354, whole genome shotgun sequence.
ACCESSION   DAOSYS010000044 DAOSYS010000000
VERSION     DAOSYS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17908
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2600_length_17908_cov_5.662354"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLU99_04305"
     CDS             complement(<1..808)
                     /locus_tag="PLU99_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16312.1"
                     /translation="MTGRLRRDPIVPWRSGGALLLILLACGGCALMRAQARLHLAAGE
                     KDFSARNIDAALVEFREAVKLDPEFAEAHSRLGVALKEKGDLTAAAEALKTAVRLDPG
                     DFSATFELGEVYRLLNNLAAAIRAYLVACELDPANFEARTSLAGCYYRNGELDSAAAA
                     YEAALKLNPKNALVWSNLGAVYHAQAKPYEALRAYKESLECDARQPAVLVNMATVYLQ
                     QERWQTAHRTLLVALDLDPNFSPAHERLGYCLWRQQLYAAAADAYQRALEL"
     gene            1023..>1460
                     /locus_tag="PLU99_04310"
     CDS             1023..>1460
                     /locus_tag="PLU99_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16313.1"
                     /translation="MVIDFFLIGVSLAAGAWGGAARSGSIAPAKPNLPKASAETAIVQ
                     RVAPLGFCAASGGCDEYISRVQIGGIDQASACAGYEDRSGLWALVTPGKSYLLTVTNG
                     NPVYPDDCCTVWVDWNQDHVWSPDERSEILTGVGPYQFTLTVPP"
     assembly_gap    1461..1560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1561..3514
                     /locus_tag="PLU99_04315"
     CDS             <1561..3514
                     /locus_tag="PLU99_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16314.1"
                     /translation="ALIPACGNPGACCSDVLAVCTDDVPELSCQGTRFAVEGQCAELD
                     PLCGTQLFTFFFDPGTGPPPATVGGLEMLPLDPDPRPLFDDVTSILTPCPGGTTIGFS
                     IPLNHRRIGAGWGWWSHGYSGDVYYTNGHSYVTLYPPPGTCALYFYVEPNTYSLESFR
                     LTVNEAVDSEVFSVHGASAAYVGVCGTELRMIRVTCTSEADFGLGEFGIACYCGVELC
                     ACCHEATGECTDDVDVLICAADPDARFIINGTCNQFLPPCGTVRGACCYGETCAVERP
                     SECSGEYMGDNWPCRPNPCLCADTVVCAPGTWTGTTCGLHDICDMSSWPEVFYEVQIP
                     HLGLWRIDLCESFVQVLLAFGETCCHTFAYEQGACGNQPYLERVLPKGNYFVTIEGWT
                     GCGIYKLKVFELPWGACCVTDTALCRDYVVPEDCYGTHTMHEFCADLEPPCTGPLGAC
                     CDDASATCTETALTLCTGRFAPLTECSELIPVCGRWSSCTHRIELYDSYGDGWTGNTL
                     DLYVNDAPVLAGLTLAGGAGPESYYFEAARADEIRTVYHPHGGWPEEAWYMIISGRET
                     VLARDGAGVLPPSGVVTAGDCPEPCGDLDLDFDVDLYDYDLFFDSFGTGRGESGYFEP
                     ADLDHDGYIGMSDLELWLGCYEAYAD"
     gene            3924..6665
                     /gene="mutS"
                     /locus_tag="PLU99_04320"
     CDS             3924..6665
                     /gene="mutS"
                     /locus_tag="PLU99_04320"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOO16315.1"
                     /translation="MPASVPLRDGERLTPAMQQYWQQKQQVGQAILLFRMGDFYEMFY
                     EDAELAARVLGIMLTSRDGGRTPLAGIPHHALESYLAKLVAAGYKVAISEQIEDPRQA
                     KGIVDRAIVRIVTPGTLTDEALLDRTRSNLVAAVCLQHGAGGIAALELSTGDFHVRMC
                     PPQQIVDELCRLEPAEVLLADDAAAGPHALEADIRDRVRAAITYRSPTDFSVSRAEQV
                     LKDHFGIARLDGLGFDHVDLSLQAAAALLVYVRETQKLTARHLCPPRREPIEDYIVLD
                     ATTLRSLEIERTLRSGARAGSLLAAVDRTCNPMGARLLRAWLCYPLKDIEQIRARQRI
                     LAALYEHPPQRSVLREALREMGDLGRTIGRLGVQRTHPRELRGLGDGLTKFAPLRTAL
                     RALKCEECDALADKLEDLDELAAILCTALNKDAPLTLREGGIFAAGHHAELDRLRAIN
                     VDGQRWLAEFQARESRRTGIPSLKVAYNKVFGYYIEITNAHRERVPPDYVRRQTVKNA
                     ERYITDELKRYEAEALSADARARELEYELFVELREQVARHIPMLQRAAGALAEIDVLA
                     GWAELAQQRKYVAPEFVAEPVLEITAGRHPVVEQALEAAFVANDTWLVAREDLGTAGK
                     GSRDLGIEGSRDRGIEGSRKDGSEGEPSDRASAKSLALITGPNMAGKSTYIRQVALLT
                     LLAHCGCWVPARRMRLGLVDRIFTRVGAADELARGQSTFMVEMLETANILHNATSRSL
                     VILDEVGRGTSTFDGVSLAWAITEHLGTRIGCRTLFATHYHELTELGELLESVFNLNV
                     VVREYEDQVVFLHRIAPGAADRSYGLHVAKLAGVPRPVLERANAVLTELEKTFSRESQ
                     RPVLAAVQRRRQRQLRLYEEPEDAVVRELRECDLKQLDTVGAVELIRRWRVILGMSE"
     gene            6729..8111
                     /locus_tag="PLU99_04325"
     CDS             6729..8111
                     /locus_tag="PLU99_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16316.1"
                     /translation="MSWLKRPEVWFPAALILLIVAGAALLNNPTCQSLDERDWRWYAC
                     ANGWRSTFDAVSAACGAGLLTHDIDEEYTTIGKCVLFAIGLIGAGLWLAAILSVSGRF
                     WRAIGAEHPLPTARRVIGAFAALVGVSTAATMLLEWLIQATTEASTPGGGLSGSIWRG
                     ISASASLGWLPGSPGRAYTWVYAVIGLVGGLGWMVWLLPFAKARREYLRVSAVAKLAG
                     GYVLYLLLAAGIMFLLESPRGLRATSVPENQLTGQQALVRYGRSLTTTVCAATAGLPV
                     ERPTEGNLTDGSRFLLGCEVLLGSLGGSPGGGIKWTLFAWFIGGGLAATCMRRQSWTP
                     TAVRRFTAAAGCIGLLVGVTLATAFGLLVIETRTARSYQEQPSFAAAFLDASSAVGGA
                     NLSAGVVRAVTGQNLSRGIGQGVDSYQYGMVLLMAAMFVGRTLLVIALSWFGGLRLTE
                     SESTRAAAVL"
     gene            8254..8922
                     /locus_tag="PLU99_04330"
     CDS             8254..8922
                     /locus_tag="PLU99_04330"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOO16317.1"
                     /translation="MKVVGLIPARAGSKRLAHKNLALLDGKPLIAFTCEAALCSGVLS
                     AVYVNTDSPAIATVAERFGVHCPALRPESLARDDTPTQQSNRFLLDLLAARGEEFDAV
                     MVLQPTSPLRTAEDIREAWELFETNQPCAVVSASPVAPASWLGRVGKDGRLEPLPGED
                     VLYELNGAIYVYTSGDYRAGRSPARTLVYPMSRSRGVDIDTLEDLKYAEFLLRQTESA
                     ACSV"
     gene            8969..10033
                     /locus_tag="PLU99_04335"
     CDS             8969..10033
                     /locus_tag="PLU99_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOO16318.1"
                     /translation="MQDQSAAPRVNIGRRAIGAGCPTYVIAEAGVNHNGSAERALRLV
                     DAAVAAGVDAVKFQVFRADELTTQTAKVAAYQKSTGARSQREMLRRLELSDAAFERIA
                     AHCRQCSIEFLATPFSVADLERLLFFGVRAVKIASTDLNNAPLLRAAADSGLPLLCST
                     GAATAAEIRGAVRRLRGRGAGSRLVLLHCISSYPTPLEAANLRVIAALRNEFGVPVGF
                     SDHTVQPQTGAWAVIAGACVLEKHFTLDREARGPDHAMSLNPAELAEYVVQVRRAEAA
                     LGTGTIGMSPIEAEVRAVARKSVVTARALRAGTVLTPDMLTIKRPAGGIEPDQLDTLI
                     GRTVTTNIGRDELLTWDVIQ"
     gene            10069..11244
                     /gene="neuC"
                     /locus_tag="PLU99_04340"
     CDS             10069..11244
                     /gene="neuC"
                     /locus_tag="PLU99_04340"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOO16319.1"
                     /translation="MITGTRSEYGILRSVMAAIAAEARLELQVVVTGMHLLPEFGRTE
                     SDIARDGWQIAARVPMQRGDDDALDQARGLARGVAGIADFLVAANTDIVVVLGDRIEA
                     MAGALAAVTTGRVLAHIHGGDVAAGDFDESLRHAITKLAHLHFAATRRSAQRIIRMGE
                     RPEYVFLVGAPGLDRLRELIDETNSGRAGQARKTRAGRTFAELRLAGQRARTALVVYH
                     ASGRAAAVEERGMHTVLSAVKKTGLRRFIVYPNTDRGHRGVLRAIRRHAVEDEGNGAV
                     TVARSLARDDYLRALLAADVLVGNSSSGIIEAPLAGTPSVDVGDRQAGRQPAGPSVIH
                     AAENERAIRAAIKEALATRPRRGGSNVYGDGRAGQRIAGILAQVALSDGLRRKQIAY"
     gene            complement(11358..11999)
                     /locus_tag="PLU99_04345"
     CDS             complement(11358..11999)
                     /locus_tag="PLU99_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16320.1"
                     /translation="MIRPRASSPLYSLAPCGAALLLLTGCHAQQVTAQFRLGYDEAIR
                     RLYEHHALMNLACAEEGAFFVQMAYSNFGSQIAVTGSISAQFTIIDTVDAPYGRDQVV
                     VDISDQTFAPTLSASQTNVLGLTASPAPNQHAIRELYQAEVNKPPEERFFFMTANPKA
                     VLGAYLSIPYRWHRWIVVPDERKREFADFVYRVSFYDPQASPVTETQTNDAPE"
     gene            complement(12049..12375)
                     /locus_tag="PLU99_04350"
     CDS             complement(12049..12375)
                     /locus_tag="PLU99_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16321.1"
                     /translation="MNGSAASEPSILTSRRKIDQTLGPFYARRRVPLGKVVASGVGGP
                     FWALVPLLLAWPALAFVRGPIRRRRRRRRGCCTSCGYPLAGLPGPRCPECGKAASPGA
                     TKTAAN"
     gene            12397..12588
                     /locus_tag="PLU99_04355"
     CDS             12397..12588
                     /locus_tag="PLU99_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16322.1"
                     /translation="MYDAGDQGQAYAGLVVLSSDPQRVPGDAMFVADQSEEQKRQTGD
                     RQGGQSEVAEEFHWVCILT"
     gene            complement(12654..12917)
                     /locus_tag="PLU99_04360"
     CDS             complement(12654..12917)
                     /locus_tag="PLU99_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16323.1"
                     /translation="MSRCKEFCRECAIKERCHNTCGCLNWQTTGSINGISPVLCRYEQ
                     MLLPIADRVGEVLYKKRDPLFLHKHYNSAYPVLSLLEDTLTTK"
     gene            complement(13129..13572)
                     /locus_tag="PLU99_04365"
     CDS             complement(13129..13572)
                     /locus_tag="PLU99_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16324.1"
                     /translation="MENLPPHLVHRQKRPSYPTKLQAAADAELLKQHLPPQWLSHREL
                     AAVAGALLAANVAGCGGGRTAPLVAPVFEHGEGKFANPQALITPLAGTPAWPAYVTEE
                     AFLSHDEALGIIAEELAQAGLSMTNRDVRLDRVVIRGHDVRKAYD"
     gene            complement(13677..14204)
                     /locus_tag="PLU99_04370"
     CDS             complement(13677..14204)
                     /locus_tag="PLU99_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16325.1"
                     /translation="MRGNECEILLEALRGSNYINALPPSDRDDVLQATLELIVSKYAH
                     LREPDLSRVGFKIAKLLALAAFRGRSRIHAQPLAKETPAPDPGPAERLEHKEKEELLA
                     RLRAALHERLQNALTPEQREAVFRRFNRNEKLREIAAALNLSVSGAHHLIESALLVLH
                     AELAEYRELWPTLNR"
     gene            14324..14689
                     /locus_tag="PLU99_04375"
     CDS             14324..14689
                     /locus_tag="PLU99_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16326.1"
                     /translation="MSDVVQISWGKDVAEKLEHLCEQQKSAAVMLPSGRRLFVCPLED
                     FAALHSVFTDPDFVGSLERAVADFAEHRGSVRGAGVRPGAEREALSDPGFVQSLERAA
                     EDLMNKRGSIREAGQRPAP"
     gene            complement(14690..15049)
                     /locus_tag="PLU99_04380"
     CDS             complement(14690..15049)
                     /locus_tag="PLU99_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16327.1"
                     /translation="MRFFWTEEADRQYLKHYADREARRAFDGLLGFIEDDPTRGSEIR
                     LIELSGDEWERRAAKALRGVIDDARLVLASVHRSAVRSEPHVLAAVVHSPAVPPGVAA
                     DHTIYTLGLVLRTNKFD"
     gene            15372..16676
                     /locus_tag="PLU99_04385"
     CDS             15372..16676
                     /locus_tag="PLU99_04385"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOO16328.1"
                     /translation="MRHKDLGNTCVLGLQWGDEGKGKVVDLLVEHFDVVARYAGGANA
                     GHTVVIDSDRFALHQVPSGVLREGVMNIIASGAVIDPAALLGEIESLRQRGVRLDGDN
                     LRISDRAHLVFPWHRREDVIAEGAARAGDKIGTTARGIGPCYADKAGRRWGIRVCELC
                     RPAGLRERILATCAYKNALFRAVYECRETFDANAITAEYLAFGEQLRPFMCDATAVLN
                     GLIRQGKRVLFEGAQGTLLDVDHGTYPFVTSSSTTNFAAGAGVPASAANSILGVAKAY
                     TTRVGHGPFPTEQAGEVGDIIRERGGEYGTTTGRPRRCGWFDAVATAYAARVCGPTHL
                     AIMHLDTLSGMRELRVCVGYRIGKKIVNDFPADVYSLEEAEPVYETLPGWSGEIGDCR
                     EFSKLPDEARRYVEFISARLETPVRIISVGPARDQTIILEDN"
     gene            16723..17517
                     /locus_tag="PLU99_04390"
     CDS             16723..17517
                     /locus_tag="PLU99_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOO16329.1"
                     /translation="MPTMPPLDPREVLGLSPEALPRHVAIIMDGNGRWARQRGLPRIE
                     GHRQGAQAVRAIVESCARLGVQVLTLYSFSLENWKRPQDEVTALMALYAEYLAAKRPE
                     LMDNDIRLLQVGRRSGLPSAVLSELERTMELTRGNQRMTLCLALNYGSRAEIIDCVRS
                     IAGRVQRGELKPEDITEQTISDGLYTAGLPDPDLLIRTAGEMRISNFLLWQISYAELY
                     VTPVLWPDFRPPDLYEALRAYGQRERRFGAVLDASPARPADRGGQA"
     gene            17559..>17908
                     /locus_tag="PLU99_04395"
     CDS             17559..>17908
                     /locus_tag="PLU99_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16330.1"
                     /translation="MLKLRITFGVLMAAAFVGLLLVDGWLAARTPESTLGRWAGNGAI
                     STALVGVLIWLAARELTMFAEACGYRPSRTLAQVGSVALAVLPYFAFNSGADRGANGG
                     WEMLVLALVVGLAFL"
BASE COUNT         3083 a         5357 c         6112 g         3256 t
ORIGIN      
        1 ccagctcgag tgctctctgg taagcatcgg cggcggccgc atagagttgc tggcgccaga
       61 gacagtaacc aagccgctcg tgagcgggcg aaaaattggg gtccaggtcg agcgcgacca
      121 gtaacgtgcg gtgggccgtc tgccagcgct cttgttggag gtaaacagtc gccatgttca
      181 ccagaacggc cggctgccgg gcgtcgcatt ccagcgattc cttgtaggcc cgcaaggcct
      241 cgtaaggctt ggcctgggcg tggtacaccg cccccaaatt gctccaaacc agggcgttct
      301 tcggattgag tttcaacgcg gcctcgtacg cggccgcagc cgaatccaac tccccgttgc
      361 ggtagtagca gcccgccaga ctggtgcggg cttcgaaatt ggccggatca agctcgcagg
      421 ctaccagata ggcccgaatg gccgccgcca aattgttcag caggcggtag acctcaccca
      481 actcgaaggt ggctgagaaa tcgcccgggt cgaggcgtac ggccgtcttg agcgcctccg
      541 cggcggcggt caggtcgccc ttttccttga gggccacccc taagcgtgaa tgagcctcgg
      601 cgaattcggg atcgagcttg acggcctcgc ggaattccac gagagccgcg tcgatattgc
      661 gagccgaaaa gtccttttcg cctgcggcaa ggtgcagccg ggcttgagcg cgcatgagag
      721 cacagccgcc gcaggccaag aggattagta gcagcgcacc gccgctccgc cagggcacga
      781 tcggatctct gcgcagtctg ccagtcactt tgttcgcaac tccaaacgat ggtcccaagc
      841 ggtatttacc agcagggcgg tggcggcgtc aacgcggccg aacacggggc gggcgttgcc
      901 aagagattat gggacactcc ggggcgggtt ctgcgctccg gtattgtggc tctcgaaata
      961 atcgatagac tgtgtgtagt cctgttgggg aaggcatgtg aaggcctgtc tggaggagaa
     1021 cgatggtgat cgatttcttc ttgattggcg tttcgcttgc agcaggcgcg tggggcgggg
     1081 cggcccggtc cggcagcata gcaccagcta agccgaattt acccaaggca tctgccgaaa
     1141 cagcgatcgt gcagcgagtt gcaccactag gtttctgcgc agcgtcaggg ggctgcgatg
     1201 agtacatcag ccgcgtgcag atcggcggca ttgaccaggc cagcgcgtgc gccgggtacg
     1261 aggaccgctc cggcctgtgg gcgctggtca cacctggcaa aagctatctg ctgactgtca
     1321 caaacggcaa cccggtgtat cccgatgact gctgcacggt atgggtcgat tggaatcagg
     1381 atcacgtatg gagtcctgac gaacggtctg aaattttgac tggcgtgggg ccatatcagt
     1441 tcacgctaac cgttccgccg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 tgccctgatt ccggcttgcg gaaacccggg cgcctgctgc tcggatgttc tggccgtttg
     1621 tacggacgat gttccggagt tgagttgtca ggggacgcgc ttcgcggtgg aagggcagtg
     1681 cgcggagctt gatccgttgt gtggaacgca gctattcacg tttttcttcg acccgggaac
     1741 gggcccgccg cccgctacgg tcggtgggct ggagatgctg ccgctggacc ccgatccgcg
     1801 ccccctgttc gacgacgtga cgtccatcct tacgccctgt ccgggcggca cgacaatcgg
     1861 tttttcaatt ccgctcaacc atcgccgcat cggggcaggt tggggctggt ggagccacgg
     1921 ctacagcgga gacgtgtatt acacaaacgg tcacagctac gtcacgttgt atccgccgcc
     1981 cgggacgtgc gctttgtact tctacgtgga accgaacacc tattcgctcg agtccttccg
     2041 gttgaccgta aacgaggcgg tcgactcgga agttttttcg gttcacgggg catcggccgc
     2101 gtacgtaggc gtctgcggca cggaactgcg gatgattcgc gtgacttgca ccagcgaggc
     2161 ggacttcggc ctgggggagt tcggaatcgc atgctactgc ggcgtcgagc tgtgcgcctg
     2221 ttgccatgag gcgacggggg agtgcacgga cgacgtagac gtgttaatct gcgcagcgga
     2281 ccccgacgcg cggttcatca taaatgggac gtgcaaccag tttctcccac cctgcggcac
     2341 ggtgcgcggc gcctgttgtt acggtgaaac ctgcgccgtg gagcggccgt cggagtgcag
     2401 cggcgagtac atgggggata attggccttg ccggcccaac ccgtgcttgt gcgcggatac
     2461 ggtggtctgc gcgccgggca cgtggaccgg gactacttgt ggcctgcacg atatctgcga
     2521 catgtccagc tggccggaag tcttctatga ggtgcagatt ccgcatctgg gtctatggcg
     2581 aatagacttg tgcgaatcct tcgttcaagt cctactggcg ttcggcgaga cctgctgcca
     2641 cacgttcgcc tacgaacaag gcgcatgtgg gaaccagccc tacctggagc gcgtccttcc
     2701 aaagggcaat tatttcgtta ccatcgaggg ctggaccggc tgcggcatct acaaactgaa
     2761 ggtcttcgaa ttgccgtggg gggcgtgctg cgtcacggac accgccttgt gccgggatta
     2821 cgtcgtgccg gaagactgtt acggtacgca cacaatgcac gaattctgcg ctgatctgga
     2881 accgccgtgt accggtccac tgggggcctg ctgcgatgat gcgagcgcga catgcaccga
     2941 gacggccctg accctgtgca ccgggcgttt cgcgccgctg acggagtgca gcgagctcat
     3001 tccagtttgc ggaagatgga gctcgtgtac gcaccggatc gaactgtacg acagctatgg
     3061 cgatggttgg accggaaaca cgctcgatct gtatgtgaac gacgcacccg tgttggccgg
     3121 gctcactctg gctggcggcg caggacccga atcgtactat ttcgaggccg cccgggccga
     3181 cgaaatccgg accgtctacc atccccacgg tggctggccg gaagaggcct ggtacatgat
     3241 catcagcggt cgggagactg tcctggccag ggatggcgcc ggggtgctgc cgccgagcgg
     3301 cgtggtgaca gccggcgact gccccgagcc gtgcggcgac ctcgatctgg actttgacgt
     3361 tgatctttac gactacgatc tcttttttga cagcttcgga acgggccgcg gcgaatccgg
     3421 ctacttcgag cctgcagatt tggaccacga cggctacatc ggtatgagcg atcttgaatt
     3481 gtggctaggc tgttacgaag cgtacgcaga ctgagccacc ggcgtcgggt gccatgtcca
     3541 agtcctgcga tagcggggcg cggcatgcga tcgtcggtta ccccgaatcc gacgccgtga
     3601 accaaggggg tatgcccgcg cttacagctt gggcatggca cccgcgcatg gcatccgtgg
     3661 cgtcagccat ggcgccgcat ggcacgtgtt cgcatgcgac gacacttccg tgcgcgcggc
     3721 gcgtcggcta cattctcgcc gtggcgagcg aagaccccaa gtgcgatctg ccagccgagg
     3781 cctcgcgcga tgcgagcgcg cggcttgcgc cgtctgccgc cccatcggca aatagcgcgc
     3841 ggcccgccgg gacgccgccc aatagcgagc gctctacgcc ggctgcgccc tctgcggccg
     3901 cgccggccaa tagcgggcgg tccatgccgg cctcggttcc gttgcgggac ggcgagcggc
     3961 ttacgccggc catgcagcag tattggcagc agaagcagca ggtcgggcag gccattctgc
     4021 tgtttcgcat gggcgatttc tacgagatgt tttacgagga cgccgagctg gcggcccgtg
     4081 tgctggggat tatgctcacc agccgcgacg gcggccgcac gccgctggcc ggcattccgc
     4141 accatgcctt ggagagctac ctggcgaagc tggtggcggc gggctacaag gtcgccatca
     4201 gcgagcagat cgaggatccg cggcaggcca aggggatcgt tgaccgagcg atcgtacgga
     4261 tcgtcacgcc gggcacgctg acggatgaag ccctgctgga ccgtacgcgc agcaacctcg
     4321 tggccgcggt ttgcctgcaa cacggggccg ggggcatcgc cgccctggag ctgtccacgg
     4381 gcgactttca cgtacgaatg tgcccgccgc agcagattgt cgacgagctt tgccgcctcg
     4441 agccggccga ggtgctgctg gcggacgatg ccgcggccgg tccacacgcg ctggaggcgg
     4501 atatccgtga ccgcgtgcgg gccgcgatta cgtatcgcag tccgaccgat ttttccgttt
     4561 cgcgggccga acaggtgctt aaagaccact tcggcatcgc gcggctcgat gggctgggct
     4621 tcgatcatgt ggacctctcg ctgcaggccg cggcggcctt gctggtctat gtgcgcgaaa
     4681 cgcaaaagct gacggcgcgg cacctctgcc cgccgcgccg ggaacccatt gaagactaca
     4741 tcgtgctgga cgcgaccacg ctgcgctcgc tcgaaatcga gcgcacgctg cgcagcggcg
     4801 cgcgggccgg ctcgctgctg gcggccgtgg atcgcacgtg taatcccatg ggggcgcggc
     4861 tgctgcgggc gtggctgtgc tacccgctta aggacattga gcagattcgc gcgcggcagc
     4921 gcatcctggc ggcgctgtac gagcacccgc cgcagcgcag cgtgctgcgc gaagcgctgc
     4981 gcgagatggg cgatctgggg cgcaccatcg gccggctggg cgtgcagcgt acgcacccgc
     5041 gagaactgcg cggtctggga gacggcctga caaagttcgc cccgctgcgc actgcattgc
     5101 gggcgctcaa atgcgaagaa tgcgatgcac tggccgacaa gctcgaagat ttggacgaat
     5161 tggccgcaat attgtgcaca gcactgaaca aggacgcccc gctgacgctg cgtgagggcg
     5221 ggatattcgc ggcggggcac catgctgagc tcgaccggct gcgcgccatc aacgtcgacg
     5281 ggcagcgctg gctggccgag tttcaagcgc gcgaaagccg gcgcaccggc attccatcgc
     5341 tgaaggtcgc ttacaacaag gtctttgggt actacatcga gatcaccaac gcccaccgcg
     5401 aacgcgttcc gccggattac gtgcgccgcc agaccgtcaa aaacgccgag cgctacatca
     5461 cggacgagct gaaacgctat gaggccgagg cgctgtcggc cgacgcccgg gcccgcgagc
     5521 ttgaatacga gctgttcgtc gagctgcgcg aacaggtcgc ccgccacatc ccgatgttgc
     5581 aaagagccgc cggtgccttg gccgagatag acgtgctggc gggctgggcg gaattggcgc
     5641 agcagcgcaa gtacgtggcg ccggaattcg tggcggagcc ggtgctggaa atcacggccg
     5701 ggcggcaccc ggtggtcgag caagcgctgg aagcggcgtt tgtggcgaat gatacttggt
     5761 tggtggcgcg ggaggatttg gggacagcgg gcaagggatc tagggatcta gggatcgagg
     5821 gatcaaggga tcgagggatc gagggatcaa gaaaggacgg aagtgaggga gaaccgagcg
     5881 atcgcgcctc tgccaaatct ctggccctga tcactggccc caacatggca ggcaagagca
     5941 cctatatccg ccaggtggca ctgctgacgc tgctcgccca ttgcggctgc tgggtgccgg
     6001 cgcggcgcat gcggctgggg ctggtggatc gcatattcac gcgcgtcggc gcggcggatg
     6061 aactggcgcg cggacaatcc acgttcatgg tcgagatgct cgagaccgcc aatattctgc
     6121 acaacgcgac ttcgcgcagc ctggttattc tggacgaagt cggccgtggc acgagcacct
     6181 tcgacggcgt ctcgctggcc tgggccatca ccgagcacct gggcacgcgc atcggctgcc
     6241 gcacgttgtt cgccacgcac taccacgagt tgaccgaact gggcgagctg ctcgaatcgg
     6301 tgttcaacct gaatgtcgtc gtccgcgagt acgaggacca ggtcgttttt ctgcaccgca
     6361 tcgccccagg cgccgccgac cgcagctatg gcctgcacgt ggcgaagctg gcgggcgtgc
     6421 cccggccggt gctcgaacgc gccaatgccg tgctcaccga acttgaaaag acgttttcac
     6481 gcgaatcgca gaggccggtg ctggcggccg tacagcgccg gcggcagcga caactgcggt
     6541 tatatgaaga gccggaggac gccgtggtgc gcgaattgcg cgagtgcgac ctcaaacagc
     6601 tcgacacggt gggcgccgtg gagttaatcc gccgctggcg ggtgattctg ggaatgtccg
     6661 aataagcgat cgatcaggaa atgcgccgcc tgcaattcca atcggcgtga ggcgagttga
     6721 gctacgcgat gagctggttg aagcgccctg aggtttggtt tccggccgcg ttgattctgt
     6781 tgatcgttgc gggtgccgcg cttctcaaca atccgacgtg ccagtcgctg gatgagcggg
     6841 actggcgctg gtatgcgtgt gccaacggct ggcgttccac gttcgacgcc gtcagcgctg
     6901 cttgcggcgc cggactgctc acgcatgaca tcgatgagga atacaccaca atcggtaagt
     6961 gcgttctgtt cgcgatcggc ctgatcggcg cggggctctg gctcgccgca attttgtcgg
     7021 tttcgggccg cttctggcgc gcgatcggag cagagcatcc gctgccgacg gcacgccgtg
     7081 tgatcggcgc gtttgcggcc ctggtcgggg tcagcactgc cgccacgatg ctgttggaat
     7141 ggttaattca agccacgact gaagcttcga cgcccggtgg aggtctgagc ggctcgatat
     7201 ggcgcgggat atcggccagc gcctcactcg gctggcttcc tggcagtccg ggacgggcat
     7261 acacatgggt ttacgccgtc attgggctgg taggggggct ggggtggatg gtttggctgc
     7321 tgccatttgc caaagcgcgg cgtgaatact tgcgggtatc ggcagtggca aagctcgcgg
     7381 gcggctacgt gctttatctg ctcctcgccg cgggaatcat gtttctgctg gagagtccgc
     7441 gcgggctgcg cgcgacgagc gtgccggaga accagctaac cgggcagcag gctcttgttc
     7501 gctatggccg cagtctgacc acgacggtct gcgccgccac cgccgggctg ccggtcgaaa
     7561 ggcccacaga aggcaacctc accgatggct cgagattttt gctgggctgc gaggtcctgc
     7621 taggttcgct gggcggttcg cccggcggcg ggattaaatg gaccttgttc gcctggttca
     7681 tcggcggcgg cctggcggcg acctgtatgc ggcggcaatc gtggacgccg acggccgtgc
     7741 gacggtttac cgccgctgcc ggatgcattg ggctgctcgt gggcgtgacc ctcgcgacgg
     7801 ctttcggctt attggtcatc gaaacccgca cggcccggag ctaccaggag caaccgagct
     7861 tcgctgccgc cttcctggac gccagctccg cagtgggggg cgcaaacctc tccgccgggg
     7921 tggtgcgcgc agttaccggt caaaacctct ccagaggaat cggccaaggt gtcgattctt
     7981 atcagtacgg catggtcctg ctgatggcgg ccatgttcgt cggacgcacg ctgctcgtga
     8041 tcgcactaag ctggttcgga ggattacgcc tgaccgaatc ggagtccacg cgagccgcgg
     8101 cggtcttgtg atcgcgaaat agtggcggat ggcggacggc caacgagccg gcggctgcta
     8161 taggagttgt agcggcctgc ggctcgcagg ctgttggctc cattggagct ttcgggactg
     8221 atttcaggac ttgattgtgg tttgggcctg catatgaaag tggtagggct gataccggca
     8281 cgtgccggtt ccaaacgact cgctcacaag aacctggccc ttttggacgg caagccatta
     8341 attgcattca cctgcgaggc cgctctctgc agcggcgtcc tctcggccgt gtatgtgaac
     8401 accgacagtc cggcgatcgc gacggttgcg gagcgtttcg gcgtgcattg cccggccctg
     8461 cggcccgagt cgctggcgcg ggatgacacg ccgacgcagc agtcgaatcg ttttctcctg
     8521 gatttgcttg cggcgcgggg cgaagaattc gacgcggtga tggtgcttca gccgacgtcg
     8581 ccgcttcgca ccgctgagga cattcgcgag gcctgggaac tgtttgaaac gaatcagccg
     8641 tgcgccgtgg tatcggcttc gccggtcgcg ccggcatcct ggctgggacg cgtcggaaaa
     8701 gacggtcggt tagagccttt gccgggcgag gacgtgctgt atgaacttaa tggcgccatt
     8761 tacgtctata caagtggcga ctaccgcgcc ggccggtccc cggcgcgcac gctcgtttat
     8821 ccgatgtccc gctcgcgcgg cgtggatatc gacaccttgg aagacctgaa gtatgccgaa
     8881 tttctcctgc gacagaccga gtccgccgcc tgctcggtct gatcgtcggc gcgcgaagcg
     8941 gagcttcgca gtgtgggaga gcggagctat gcaagaccag agcgccgccc ctcgcgtgaa
     9001 tattggccgc cgggccatcg gtgcgggctg ccccacgtac gtgatcgccg aggccggagt
     9061 caaccacaac ggctcggccg aacgggcgct gcggttggtc gatgccgccg tggccgcggg
     9121 cgtcgatgcg gtcaagttcc aggtttttcg tgctgacgag ctaacgacgc aaacggcgaa
     9181 agtggcggcg tatcagaagt ccacgggggc gcgctcgcag cgcgagatgc tcaggcgact
     9241 cgagctgtcc gacgcggctt tcgaacgtat tgcggcacac tgccggcagt gctcgatcga
     9301 gtttttggcc acacccttta gcgtcgccga tctggaacgc ctgctgtttt tcggcgtgcg
     9361 cgccgtgaag atcgcttcga cggatctcaa caatgcgccg ctgctgcggg ctgccgctga
     9421 cagtggactg ccgctgctat gctcgaccgg cgctgctacg gcggcggaaa tacgtggcgc
     9481 ggtgcggcgg ctgcgcggtc gcggtgcagg cagccggctc gtgctgctgc actgcatcag
     9541 cagctatcct acgccgcttg aggccgcgaa cctgcgggtt atcgccgcgc tgcgaaacga
     9601 gtttggggtc ccggttggat tcagcgacca cacggtgcaa ccgcagaccg gcgcctgggc
     9661 ggtgatcgcc ggggcatgcg tactggagaa gcacttcacg ctcgatcgtg aggcccgggg
     9721 cccggatcac gccatgtcgc tcaacccggc cgagctggcc gagtatgtgg tgcaggtgcg
     9781 ccgcgccgaa gcagcactgg gaaccggcac gatcggcatg tcgcccatcg aagccgaagt
     9841 gcgcgccgtg gcacgcaaga gtgtggttac ggcccgtgcg ttgcgcgccg gaaccgtgct
     9901 cacgccggac atgctgacca tcaagcggcc cgccggcggc atcgagcccg accagctcga
     9961 caccctcatc ggtcgcacag tcactacaaa cattggccgc gacgagcttc tcacgtggga
    10021 cgtgattcag tgagccgcag gccacgcacg ccacgtcggc gcgtcgccgt gatcaccggt
    10081 acgcgctcag agtatggcat cttgcgttcg gtcatggcgg ccattgcggc cgaggctcgg
    10141 ctggaattgc aggttgtagt cacggggatg catcttctgc ccgagttcgg gcggactgag
    10201 agcgacattg cgcgcgacgg ttggcagatc gcggcccgcg tgcccatgca acggggcgac
    10261 gacgacgcgc tcgatcaggc ccgggggttg gcgcgcggcg tggcgggcat cgccgatttc
    10321 ctcgtcgccg ccaacaccga catcgttgta gttctcggcg accgcatcga agcgatggcc
    10381 ggggcgctgg ccgcggtgac caccggccgc gtgctagctc acattcacgg cggcgacgta
    10441 gcggccggcg atttcgacga aagcttgcgc cacgcgatca ccaagctcgc gcacctgcat
    10501 tttgcggcca cgcggcgttc tgcgcagcgg atcattcgca tgggggagcg cccagagtac
    10561 gtgtttctcg tgggcgcgcc ggggctggac cggctgcgcg agttgattga tgagacgaac
    10621 tcggggcgtg ccgggcaagc gcggaaaacc cgggcgggcc ggaccttcgc cgagctgcgg
    10681 ctcgccgggc aacgcgcgcg gaccgccctg gtggtgtacc acgccagtgg gcgtgcggcc
    10741 gccgtcgaag aacgcggcat gcacaccgtg ttaagcgcag tcaagaaaac cggtctgcgg
    10801 cgtttcatcg tgtacccgaa taccgatcgg ggccataggg gtgtgctgcg agcgattcga
    10861 cgccacgcgg tcgaggacga agggaacggc gcggtcacgg tcgcgcgttc gctggctcgt
    10921 gacgattacc tgcgtgctct actggcggcc gacgttctgg tgggcaactc gtccagcggc
    10981 atcatcgagg ccccgctggc cggaacgcct tcggttgacg tcggcgaccg gcaagccggc
    11041 cggcagcccg cgggcccttc ggtgattcac gcggccgaaa acgagcgggc gatccgcgcg
    11101 gccattaaag aagctcttgc gacgcgtcca cggcgcggcg gttcgaacgt ctacggcgac
    11161 ggccgggcgg gccagcgcat cgccggcatt ctcgcgcagg tcgcgctcag cgacgggctg
    11221 cgcaggaaac agatcgccta ctgaccctaa tgtgccaaat aagcgggtgc catgcccaag
    11281 ctgtaagcgc gggcatgccc atcgctaacg gcgtcaggtg cgttcgctca cggagcacgc
    11341 gcggggttgt gcgagcgcta ctcaggagcg tcattcgtct gggtctccgt caccggtgac
    11401 gcctgcggat cgtaaaacga aacgcggtag acgaagtcag cgaactcgcg cttgcgctca
    11461 tcaggcacga cgatccaacg atgccaccgg tatgggatgg acaggtaggc gcccagcaca
    11521 gccttggggt tggccgtcat gaagaaaaac cgctcctctg ggggcttgtt cacttcggcc
    11581 tggtacagct cgcggatggc gtgctggttg ggggcggggc tggccgtcag tccgagcacg
    11641 ttggtttgag aggcgctgag cgtgggcgcg aaggtctgat cggagatatc cacgactacc
    11701 tgatccctgc cgtacggggc atcgacggtg tcgataatcg tgaactgcgc gctgatgctg
    11761 ccggtgacag cgatttggct gccgaagttg ctgtacgcca tctgcacgaa gaaggcgcct
    11821 tcttcggcgc aggccaaatt catcagggcg tgatgctcat acagtcggcg gatggcctcg
    11881 tcatagccca gccggaactg ggccgtcact tgctgcgcat ggcagccggt tagcagcagg
    11941 agcgctgcgc cgcagggcgc gagcgagtac agcggtgagg atgcgcgtgg tcgaatcata
    12001 atcgcttaat agcggaatac gcggcgtgct tcaacgctgc ccggcgggtc agtttgcggc
    12061 ggttttcgtt gccccaggac ttgccgcttt cccacattcg gggcagcgcg gcccgggcag
    12121 gcccgccagc gggtaaccgc atgacgtgca gcagccgcgg cggcggcggc gccggcggcg
    12181 gatggggccg cgcacgaatg ccagggccgg ccaggctaag agcagcggca cgagcgccca
    12241 gaacggcccg ccgacgccgc tggcgacgac cttgccgagc ggcacacgcc gccgcgcgta
    12301 aaagggcccg agggtctggt cgatcttcct gcgtgacgta aggatgctgg gctcactcgc
    12361 agccgagccg ttcaaccgcg gcgtcgagta cacggcgtgt acgatgccgg cgatcagggc
    12421 caggcgtacg cggggctggt cgtcctgagt agtgatccac agcgtgttcc cggggatgcc
    12481 atgtttgtag ctgaccagtc cgaggaacag aagcgccaga ccggcgatcg tcagggtggc
    12541 cagtcagagg ttgcggagga gtttcattgg gtgtgcattc ttacgtaaac acaccgggag
    12601 acgccggtgc cacactttcc accagggggc atcaatgccc aaccagtagc gggtcacttt
    12661 gtggtcagcg tgtcttcgag caatgatagc accgggtaag ccgagttgta gtgtttgtgc
    12721 aggaagagcg ggtcgcgctt tttgtagagg acctctccga cgcggtcggc gatcggcaga
    12781 agcatctgct cgtagcggca caacaccggc gaaatgccgt tgatcgagcc ggtcgtctgc
    12841 cagttcaggc agccgcaggt attgtggcag cgctccttga tggcgcactc gcggcagaac
    12901 tccttgcacc tgctcactca gccggcgacc aagatcaacg tgattgctta tccaacctgc
    12961 gttcacggcc ccgagggcct gatgctgatc gtctgtgcta acgccgccca gcgggaagta
    13021 atcatctttg gtcagaaatt cgacgtacac cgcgtgcttt tggttcgcca gatccgcgac
    13081 cagcggccca gcgcattcga tcgcttgcca gctccagcaa ttgtgaacct agtcatatgc
    13141 cttgcggacg tcatgcccgc gaatgaccac cctgtccagc cggacgtcgc gattggtcat
    13201 tgagaggccg gcctgggcca actcctctgc gatgattccc agcgcctcgt cgtggcttag
    13261 aaacgcctcc tcggtcacgt acgccggcca ggccggagtt ccggcgagtg gcgtgatcaa
    13321 ggcttgagga ttcgcaaact ttccctcccc gtgttcgaac accggcgcga ccagaggcgc
    13381 cgtcctgccg ccgccgcagc ccgccacatt ggccgccagc agcgcgccgg caaccgcagc
    13441 gagctcgcga tgcgacagcc actgtggcgg cagatgctgc ttcagaagct cagcatctgc
    13501 agcagcttgc agtttggtcg gatagctcgg gcgcttttgg cggtgaacga gatgtggcgg
    13561 aaggttttcc aaatcggacg cctccgcggt cggtgcgcga cgccgacgag ttctggtgca
    13621 ttataccgtg ctggcgcgcc gcggttgacg ccgagatcgc tgcgccgcgt ttgacttcac
    13681 cggttcagag tgggccacag ttcgcggtat tcggcgagtt cggcatgcag gacaagcagg
    13741 gccgactcga tcaggtgatg ggcgccggag accgagaggt tcaacgcggc tgcgatctcg
    13801 cgcagcttct cgttgcggtt gaagcggcga aacaccgcct cccgctgctc gggcgtaagc
    13861 gcgttctgga gccgttcgtg cagggccgcc cttaacctgg ccagcagttc ctctttctcc
    13921 ttgtgctcca gccgctcggc cggtcccgga tcgggagcag gcgtctcttt ggcgagcggc
    13981 tgggcgtgaa tgcggctcct gccgcggaac gctgcgagtg ccagaagttt tgcgatcttg
    14041 aaaccgacac ggctaagatc gggttcccga agatgagcgt acttgctgac aatcagctcg
    14101 agcgtagctt gcaggacatc gtcgcggtcc gagggtggca atgcgttgat gtagttggaa
    14161 ccgcgcaacg cctcgagcag gatttcgcac tcgttaccgc gcatattttc ggaccgggca
    14221 cctgggcggg gaatttcgtg aaaaaaaagc ccgccacgac attctattgt gaaggaaaac
    14281 gcccgacttg aacgcaacct tcgccgacgc aaggagttga ggtatgagcg acgttgtgca
    14341 aatttcctgg ggcaaggacg ttgcagaaaa gctggaacat ctctgcgaac agcagaaatc
    14401 tgcggcagtc atgttgccca gcggccgtcg gttgttcgtg tgcccgcttg aagatttcgc
    14461 ggcgctgcac agcgtattta ctgaccccga cttcgtgggc tccctcgagc gggccgtagc
    14521 agatttcgcg gagcaccgcg gatccgttcg gggcgcaggc gtgcgcccgg gcgccgaacg
    14581 cgaggcctta agcgacccgg gtttcgtgca gtcgctggag cgagcggctg aggatttgat
    14641 gaacaagcgc ggttccattc gcgaagccgg ccagcggccg gcgccttaat cagtcgaact
    14701 tgttggtgcg cagcacgagc cccagcgtgt atatcgtgtg atccgcagct acacccgggg
    14761 gcactgcggg cgagtgcaca actgccgcca gaacgtgcgg ttcactgcga acggcgctcc
    14821 gatgcacgct cgccaggaca agccgcgcgt cgtctatgac tccgcgaagg gccttcgccg
    14881 cgcggcgttc ccattcgtcg cccgaaagct cgatcaagcg aatctccgat ccacgagtgg
    14941 ggtcatcctc gataaacccc agcaggccgt cgaacgcccg ccgtgcctct cgatctgcgt
    15001 agtgcttgag gtattgccga tcggcttctt ccgtccaaaa gaatcgcacc gccaacctcc
    15061 tcgtgtgagc ggcgaagggc accggagcgc attttcgggc aaggaagtgg aagccacaag
    15121 cgggcgaaac tttcctgaaa gcgaagaaag cgactggatg cacgaagcgc agcgcttgct
    15181 tcgcaggtgc tgcggcgtca gatcatcgga ggggcggtcg acggccgtgg cgacgtcccc
    15241 tcgatcgctt gatccattac tctccgcgtt tgccatcggc gcgcattcat gctaaaaatc
    15301 agccgcgtta atggggctgt cctgcggcgc cgccgggcgc cgcgcgcaag cgatccgaaa
    15361 gccagagtgc gatgcgtcac aaggacttag ggaatacgtg cgttctgggc ctgcaatggg
    15421 gcgacgaggg caagggcaag gtggtcgatc tgttggtcga acactttgac gtggttgccc
    15481 gttatgccgg gggagccaat gccgggcata cggtcgtcat cgacagcgac cgcttcgccc
    15541 tgcaccaggt gcccagcgga gtgctgcgcg agggggtaat gaacatcatc gccagcgggg
    15601 cggtcattga tccggccgcg ttgctgggag aaatcgaatc gctgcgacaa cgtggcgtac
    15661 ggctcgacgg cgacaatctg cggatcagcg accgggccca cctggttttc ccctggcacc
    15721 ggcgggaaga cgtcatcgcc gaaggcgcgg cccgggcggg cgacaagatc ggcacgaccg
    15781 cccgcggcat cgggccgtgc tacgcagaca aggctgggcg gcgctggggg attcgcgtgt
    15841 gcgagctatg tcggccggca gggctgcggg aacgtattct ggcgacctgc gcctataaga
    15901 acgccctgtt cagggctgtt tacgagtgtc gcgagacgtt cgacgcgaac gcgatcacgg
    15961 ctgaatacct ggctttcggc gagcagcttc ggccgttcat gtgcgatgcg acggcagtgc
    16021 tgaacgggct gatccggcag ggcaagcgcg tactgttcga gggcgcgcag ggcacactgc
    16081 tcgacgtgga tcacggtacg tacccgttcg tgacaagttc cagcacgacc aacttcgccg
    16141 cgggggcggg cgtgccagcg agcgctgcca acagcattct gggtgtggcg aaggcgtaca
    16201 cgacgcgtgt ggggcacggc ccgtttccga cggagcaagc cggcgaagta ggcgacatca
    16261 ttcgggagcg cggcggcgag tacggcacga ccaccggccg cccgcggcgc tgcggctggt
    16321 tcgacgcggt ggcgaccgct tatgcagcca gagtatgcgg accgacgcac ctggccatca
    16381 tgcacttgga taccttgtcc ggcatgcgcg agctgcgggt ctgcgtcggt taccgtatcg
    16441 gtaagaagat tgtgaacgac tttccggccg acgtgtatag tctcgaagaa gcggagccgg
    16501 tctacgagac gctgcccggc tggagcggcg aaatcggtga ctgccgcgag ttctccaagc
    16561 tgccggacga ggcgcggcgc tacgtggaat tcatcagcgc ccggctggag actccggtgc
    16621 ggatcatcag tgtcggtccg gctcgtgatc agacgatcat tttggaagac aactgacgat
    16681 caaaggccga atgacaaacg tcacggaagg caagtagcga ttatgcccac catgccgccc
    16741 ctggatccgc gtgaagtgct gggcttgtcg cctgaagctc tgccgcggca cgtcgccatc
    16801 atcatggacg gcaacgggcg gtgggcgcgg cagcgcggac tgccgcggat tgaaggtcat
    16861 cgccaggggg cccaggccgt gcgggcaatt gtcgagtctt gcgcgcggct cggcgtgcaa
    16921 gtgctgacgc tttacagctt cagcctggag aactggaagc ggccgcagga cgaagtaacg
    16981 gcgctcatgg cgctctacgc ggagtacctg gccgccaagc gtccggaact gatggataac
    17041 gacatccgcc tgttgcaggt cgggcggcgc agcgggttgc cgtcggccgt tctgagcgaa
    17101 ctcgagcgta cgatggagct gacgcgcggc aaccagcgga tgacgctgtg cctggcgctg
    17161 aattacggct cgcgggcgga gattatcgac tgcgtgcggt cgatcgccgg acgagtgcag
    17221 cgcggcgagc tgaagcctga agacatcacc gagcagacga tctcggacgg gctctacacg
    17281 gccgggttgc ccgatccgga cctgctgata cgcacggcgg gcgaaatgcg gatcagcaac
    17341 ttcctgctct ggcagatcag ctacgccgag ctttatgtta cgccggtgct gtggccagat
    17401 tttcggccgc cggacttgta cgaagcgctg cgtgcgtatg ggcagcgtga gcggcgcttt
    17461 ggggcggtgt tggatgcgtc gccggctcgc ccggcggatc gcggcgggca agcgtgacaa
    17521 cgcgggttgg tccagaaaag gcccaggcgc gaggggccgt gctcaagctg agaattacat
    17581 tcggcgttct gatggcggct gcgttcgtgg ggctgctgct cgttgatgga tggctggccg
    17641 cgcggacgcc ggaatcaacg ctgggccggt gggcgggaaa cggggcgatc tcgacggctc
    17701 tggtgggggt gttgatctgg ctggcggcgc gcgagctgac gatgttcgcg gaagcttgcg
    17761 gatatcggcc gagccggacg ctggctcagg tgggtagcgt cgcgctggcg gttctgccgt
    17821 acttcgcgtt taacagcgga gcggatcgcg gcgccaatgg ggggtgggag atgctggtgc
    17881 tggcgctggt ggtggggctg gcctttct
//
LOCUS       DAOSYS010000045        17840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2614_length_17840_cov_5.634074, whole genome shotgun sequence.
ACCESSION   DAOSYS010000045 DAOSYS010000000
VERSION     DAOSYS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17840
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2614_length_17840_cov_5.634074"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLU99_04400"
     CDS             <1..458
                     /locus_tag="PLU99_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16331.1"
                     /translation="DGLVAIDTVSIAADERINWRLPNGRMLLTTDELRVFVLSTEGNI
                     LTVDVHTGEVFHEIATPGMTLGVPNPGRATIVVASPDGRVFCARLRGMPMVRREDLRE
                     ALTTVPPEAKAPTAEAAPPPPDVTGVPPPSPGEPPIGGRSKVSRGYGRP"
     gene            583..2244
                     /gene="purH"
                     /locus_tag="PLU99_04405"
     CDS             583..2244
                     /gene="purH"
                     /locus_tag="PLU99_04405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOO16332.1"
                     /translation="MSGTPTTQPQIRRALISVYDKTGIVDFARALHDEFGVEIISTGG
                     TATTLQQAGIPVTLVEQITGFPEMLDGRVKTLHPRIHAAILADRDNPEHMRQLKEQGI
                     EPIDMVVVNLYPFEKTIADPNCTFEQAIEMIDIGGPCLLRAAAKNHKHVAIVPNDYPA
                     YLKLLREGRFPGTAEARAANAARIFAQTASYDSRITAWLQEAAQSWPRHAPLWMLNWG
                     PTRYGENPHQKGELLSICEDASALNLARASEAGQEGAQTKLSYNNYADASAALELAAE
                     LTRAMPGKKACCFIKHTNACGVAAGPDAIETYRKAYLGDPNAAMGGILAVNFEVDAAF
                     AEVVMQTYAQWGKAAGAGGFFVEVWLAPAFAEDALQIIRTRKEWGKRVRLLAVGDMSL
                     PPDPKQMDFKRIAGGMLMQTRDLVGLNEEQWKVVTTRQPTDSQLADLRLAWLICKHTK
                     SNAITVCRDGMLLGNGAGQMSRVMSCRIATWLAKENGHAEALTGAVAASDAFFPFRDG
                     PDILIDAGVTALIQPGGSKNDQQVIDACNERSVPMIFTGTRHFKH"
     gene            complement(2297..3367)
                     /gene="hypD"
                     /locus_tag="PLU99_04410"
     CDS             complement(2297..3367)
                     /gene="hypD"
                     /locus_tag="PLU99_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOO16333.1"
                     /translation="MSARVPELCEELRGLCQRVGRRMQFMEVCGTHTVSIFRSGVRSM
                     LPPNLRLVSGPGCPVCVTAQRHIDAAIELATRPEVTIATYGDMIRVPGRRGSLERLRA
                     GGADVRVVNSTKTALQLARDNPARQVVFLGVGFETTAPATAIAVLEAARDGLENFSVL
                     MCHKLVIPAMLALLSSGDVPLDGFLCPGHVSVIIGARAYQPIVAQHRRPCVIAGFEPE
                     QILRGLINLVRQVAEGRAEVENVYAAVVSEQGNATALGLFERVFVPEDTAWRAIGVIP
                     QSGQGLAPAYRRFDAVERFGLMLGEDEDHPACRCGEVIQGKTEPANCALFGTACTPLH
                     PVGPCMVSSEGTCAAWYKYHRR"
     gene            complement(3376..3675)
                     /locus_tag="PLU99_04415"
     CDS             complement(3376..3675)
                     /locus_tag="PLU99_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOO16334.1"
                     /translation="MCLAVPAQVIEKTGDDAVVDLQGNRLRISTALTPEVGIGDWVLV
                     HAGFSITHLSEADARETWDYLRQALGDALTEAGGEPAGPEPAVLPPNRPQDESGR"
     gene            complement(3675..6161)
                     /locus_tag="PLU99_04420"
     CDS             complement(3675..6161)
                     /locus_tag="PLU99_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOO16335.1"
                     /translation="MPVNEEKVVRRRLDLRGQVQGVGFRPFVYRLATEFKLGGYVGND
                     SNGAFIEVEGSADCIAAFEAALVQRLPPLAKITELRRASLPVQAEREFRITGSRADPQ
                     RRPEVTPDAATCADCQRELFDPADRRYRYPFINCTNCGPRYSIIKTVPYDRPATTMAV
                     FKMCPACQREYDDPADRRFHAQPNACPVCGPQLRLCRVRKPQGAARGREPAWVVEALE
                     GDPIREAARMLAEGRILAVKGIGGYHLACRADREDVVRTLRQRKLRDGKPLALMVPSI
                     AAAQAFCILTPADIAALTSPAAPIVLARKKPEYCSLDPSIPSLGRWEGGKVGMWEGSL
                     DRSIPRSLDPSRPTIAPSVAEQVDTFGVMLPYAPLHHLLFAEGLGPLVMTSANLSGQP
                     LTFRDEDAFAELHEVADAFLIHDREIFRPIDDSVVVTFDAETVPIRRARGYAPRPLRL
                     EAFDGDGPLAAVRGRRILAVGGELKSTVCLLHNGEAIVSEHLGDLSNPAAYRHFVQAI
                     GRLEELFGFKPDVVAHDLHPQYLSSVFARQREVPTLAVQHHHAHVASVMAEFGVSGPV
                     IGLSCDGTGYGTDGAVWGCETLLCERGDFERLGHLEYFPLVGGDAAAIDTWRPALALA
                     VAAGGSAADVIPIASGDVPTAPEVAVFERQLASGVNAPPTSSLGRVFDGVSYLLGLCR
                     RNRHEAEAAMALEAAAGAYAENAVEAYGYEVRDHGGMLRLSMSQMIREIIAERRANVA
                     VPRIAARFHETIARLLADAAAVACERHGIRVVALSGGCFANRRLLTRVVELLASSGLK
                     VLYHRSVPSGDGGLALGQAIVAAWKTAENS"
     gene            6362..6919
                     /locus_tag="PLU99_04425"
     CDS             6362..6919
                     /locus_tag="PLU99_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO16336.1"
                     /translation="MSSDWEPGHDAALSPATPDQGTASGDAPAEFHLPHPAVRTVWML
                     SATFTAAALGVAGLLLEALWLQRATSWPLPFGVLTIAVFLLFLVSGLLGADLRYRAWQ
                     YALRHTVVLAHFGVIWRTRRCVPRSRIQHVDIESGPIDRAFGLVKLSIFTAGTIAAVV
                     VIPGLSPTDAELLRERLVGAERDHA"
     gene            6912..8507
                     /locus_tag="PLU99_04430"
     CDS             6912..8507
                     /locus_tag="PLU99_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOO16337.1"
                     /translation="MPDSTPAEERPAETGTPRPHVDSAQTGAGPEMRRLHPATILIEL
                     LRRIGSFAYIILLALLADFLGERRGSDWTEVAISGVLVLGAAGAVLRYVSVRFGIDNQ
                     RLVIRSGILNRQVRTIPLDRIQNIDLRRGVLHRLLRVVDVSIETAAGGRAEAELSALS
                     EAEAERLKAELLEVRQHAAGIAPEAHEAHMPLTEAHEGEVIWRASLSDLLLAGATENR
                     AGLIVAGLAGLWYTFGAALQGRFAEQFQRTYENWLGAGGRGTALLIIAGITLLIVAGW
                     LVSIVLAVVRYYGFVLRRRGNDLRRRYGLFTQFETVLPLGRIQLLRIEAPWPRRLLRC
                     CSVYVETAASVLEAEGGGSAALSPLVRWRRLPALCREVFPELDLEQVTWNPVSRLTIR
                     RGMIRYTVAGLIVLGLASLYLHGYALWGLPVVFVLALGAAYARFRALGYAEQGQFVLA
                     RAGVWTRRIWVVPHSKVQFVSVSQSPFQRRLKLANLEVQTAGSRVTKEARVIDLPAAK
                     AEAMQDALADRANAYGAWQPEGV"
     gene            8901..9965
                     /gene="hypE"
                     /locus_tag="PLU99_04435"
     CDS             8901..9965
                     /gene="hypE"
                     /locus_tag="PLU99_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020412780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOO16338.1"
                     /translation="MKNSTTKEIEHVVLAHGGGGELMARLIRDHLLPPLKNERLAPLT
                     DGAVLPWTAGDLVFTTDSYVVTPLEFPGGDIGRLAVAGTVNDLAVMGAEPVALSLGLI
                     LEEGLPLEALDRILASIAATAAEAGVTIVTGDTKVIERRKKPAAESPPAGPAADGLFI
                     NTAGIGRRRPGVNLDIKRIAPGDLILINGTLADHGLTIMSVRNGIEFESALRTDAAPL
                     NGLIGQLLDCGADVKFLRDPTRGGLAGVLADICEATSLSIDVAEDQVPISRTARHAAE
                     MLGLDPLTVANEGKVVAVIAPQHVEQALRALRSHPLGKSAAVIAEVTGAAPPLVELIT
                     RVGGRRIIQRPYGEDLPRIC"
     gene            10078..11319
                     /locus_tag="PLU99_04440"
     CDS             10078..11319
                     /locus_tag="PLU99_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOO16339.1"
                     /translation="MSSTSLTLLAIALTALATARLSFGGGQPTTAATRPAGEAALVKG
                     NTAFALGIYDRLRAEEGNLFFSPLSISTALAMTYAGARGATAAEMAQALHFDLPPDQL
                     HTAFAAILNELNPPQGAAEPRPYKLHIANALWAAQGHKFLPAFLKTAEQSYQAGLAEL
                     DFSQAAEQARQTINDWVAEHTAGKIKDLIPPGVLDATTKLILTNAIYFKGNWAAQFDP
                     KSTKEEPFTRADGSQVNAPLMHQTGEFGYLEQPDFQALDLPYAGNRLRMTILLPRKYD
                     GLPELEKKLTPEALAGWLGQLSEQKVQVALPKFKLTSEFRLDDALRELGMRLAFRVGA
                     ADFSGMDGTQELYIGAVLHKAYVDVNEEGTEAAGATGVVMKMRATPGPPPVFRADHPF
                     LFLIRDVQSGSILFLGRLANP"
     gene            11511..13148
                     /locus_tag="PLU99_04445"
     CDS             11511..13148
                     /locus_tag="PLU99_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOO16340.1"
                     /translation="MSTQTSISPEGEKFALPTPEQYPAEFARLKKLVDQNRAEGREIV
                     VVVGVGFVGAVMAAVVADSRDKATGKHNKFVIGMQRPSPRSYWKIELLNRGLSPVKAE
                     DPEVDQLIGRCVKETKTLCATYTHEVMKLADVVVVDVQCDYVKRDLGNLRTGTADMAA
                     LEKSLATIGEMIPPNCLVLIETTVAPGTTEYVAYPILKKAFEKRGIESEPLLAHSFER
                     VMPGRHYVASIRDFWRVCAGVNEESRRRVVKFLTEVLNTEKFPLTVMDRPLESETTKI
                     VENSYRATILAFLHEWSLFAERNGVDLIKVVNAIKMRPTHSNMIFPGPGIGGYCLPKD
                     GGLGMWAYKHLMGFEDDIFKLTEAAININDTRALHVAELVRDALRNMGKYLAASKCVL
                     LGAAYREDVGDTRYSGSELVVRKLTEMGAEVAIHDPYVQHWWELEAQDTYPAPGQSWA
                     RFFRNQEHLKDSHVDSDMATALKDADAVILAVRHEPYLRLSPDEVVKMAGKPLAVIDC
                     FGILDDAKIRRFFELGCEVKGLGRGHVKRIKDSVRGS"
     gene            complement(13290..14793)
                     /locus_tag="PLU99_04450"
                     /pseudo
     CDS             complement(13290..14793)
                     /locus_tag="PLU99_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015373940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
     gene            15099..15896
                     /locus_tag="PLU99_04455"
     CDS             15099..15896
                     /locus_tag="PLU99_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16341.1"
                     /translation="MLYAALTFWLLVIMFSAWGVHHIWAGLIKPRSVNFILLPGTLVA
                     QLGHVMGLLVTGNSVKNTQLMGDDEKGDPKSANPDRVRLPVVGPIIVGLLPLVACAVC
                     LWFAARYWGWSVIQGLNAEGVRGGLARELPTSLPAFWEFLRGAISLMERMLDGVLRSN
                     LFNWSTILFLYLAICLTVRMAPFEGNRRGAIGAVLLAGLVIGVLGSLIPSVQETLAAS
                     GPSWLILSFAVAALLFMLMISLMATGVVGLVRILAGKQEAGSAARKG"
     gene            16069..>17840
                     /locus_tag="PLU99_04460"
     CDS             16069..>17840
                     /locus_tag="PLU99_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16342.1"
                     /translation="MPNPFRAYGNAPAFFRLASIYTVVMAVLIYGGCPKPPTPITNSN
                     DNASSNANSNSSNSNSSPNNNGAVANKNGSVDNLNINVNNSNDNDVFDPEKPKPDTIT
                     FEEIVRTGDFVPDVNGTATGAVFSDFGSPIIDASGRVAFWARYTGGSGHGGLYVWIPA
                     KAGVGGTLKAVIDDSGNIPVPSATPGKPTAENFICNPATLDIRTQPLTWSGDGRLLFV
                     SAYSGPGNKLMVRWSAQQADPAKALLRVADTQAIADAMGTTVAATIVTFANIGCSDEG
                     LVFFQTHYFDISANKSGDAICKAEVRQVGGTTKLVVTPVVPVASATPSASDRGAVPEQ
                     GPSAAFVLYDHYTTLSPDGYLLFQGAYDSGTGTHGAYLRIPGDLVVRVIDSRPDSEWL
                     GLDPGVVIGNDEDFYEAIAISTNVHIAIQTPIIAGGSSVRRVILWDFALGEWTVLVTD
                     EDRAITDLLSGVSDGGALLGLAGSEPYIVGVGAAVDIAANLSSELDGVALEWMGWGAT
                     SGSINNSGHAIVPYVRVAESTQGLGFWNGEELLIIADQLLNIPDAEAAITEITAESMP
                     ETDRPGRSGILNDNDEAVFRLDLGT"
BASE COUNT         3357 a         5982 c         5372 g         3129 t
ORIGIN      
        1 atgatgggtt ggtggccata gataccgtca gcatcgcggc agacgagcgc atcaactggc
       61 gactgccaaa cgggcggatg ctgttgacca ccgacgagct gcgcgtcttc gtgctgtcga
      121 ccgaaggcaa catcctgacc gttgacgttc acaccggtga agtgttccac gagattgcta
      181 cgcccgggat gaccctgggt gtgccgaatc ctgggcgggc cacgatcgtt gtcgcctctc
      241 ccgacgggcg cgtcttctgt gcccggctgc gcggcatgcc catggtacgg cgcgaggacc
      301 tgcgcgaggc tctgaccacc gtcccgcccg aggccaaggc ccccaccgca gaggcagccc
      361 ctccgccgcc cgacgtgacc ggcgtgccgc ctccctcacc tggagagccg cccatcggcg
      421 ggcgctcgaa agtctcgcgc ggctacgggc ggccatagcg cgaccggggc ggccggctcc
      481 cgcctccagc ttgcggacgc gcaacttcac ggcgaaaatc gcattgacaa ctgaggtcgg
      541 gctgcccgtt cggcagcccg tttgttcttt cgaggtaacc cgatgagcgg cacaccaacc
      601 acgcagcccc aaatccggcg agcgttgatc agcgtttacg ataagacggg catcgtcgat
      661 ttcgcccgcg ccctgcacga cgagttcggc gtcgagatta tctcaaccgg cggcaccgcg
      721 accacgctgc aacaggccgg cattccggtc acactggtcg aacagatcac cggcttcccc
      781 gaaatgctcg acggccgcgt gaagacgctg cacccgcgaa tccacgcggc cattctggcc
      841 gatcgcgaca acccagagca catgcgccaa ctgaaggaac agggcatcga accgatcgac
      901 atggtggtcg tgaacctcta cccgttcgag aagaccatcg ccgatccgaa ctgcacgttt
      961 gagcaggcga ttgagatgat cgatatcggc gggccctgtc tgctgcgggc ggcggcgaag
     1021 aatcacaaac atgtagccat tgttcccaat gactacccag catacctcaa gcttcttcgc
     1081 gagggccggt tcccgggtac agcggaggcc cgcgctgcaa atgcggcccg catcttcgct
     1141 caaaccgcct cgtatgacag ccgaatcacc gcctggcttc aggaggccgc acagtcctgg
     1201 ccgcggcacg caccgctatg gatgttgaac tggggtccga cgcgctatgg tgaaaatcct
     1261 catcaaaagg gcgaattgtt aagcatatgt gaagacgcca gcgcgcttaa cctggctcgc
     1321 gcatccgagg ccggccagga aggcgcccag acaaagctct cttacaataa ttatgcagat
     1381 gccagtgcgg cattagaact ggcggccgag ctgactcggg caatgcctgg caagaaagcc
     1441 tgctgcttta tcaagcacac caacgcctgt ggcgttgccg ccgggccgga tgcgatcgag
     1501 acttaccgca aggcgtattt gggagacccg aacgccgcca tgggtggaat tcttgcggtc
     1561 aattttgagg tagatgccgc tttcgccgaa gtcgtgatgc agacctacgc ccaatggggc
     1621 aaggcggccg gcgcgggcgg gttctttgtg gaggtctggc tggcgccggc cttcgctgaa
     1681 gatgcgctac agatcattcg gacacgcaaa gaatggggca agcgcgtccg gctgctggca
     1741 gtcggagata tgtcgctccc gcccgatccg aagcaaatgg atttcaagcg catcgccggc
     1801 ggcatgctga tgcagacgcg tgacctggtt gggctcaatg aggagcagtg gaaagtcgtc
     1861 accacgcgcc agcccactga cagccagttg gcggatctgc ggctcgcgtg gctcatctgc
     1921 aagcatacca agagcaatgc cataaccgtc tgccgcgacg gcatgttgct cggcaatggc
     1981 gccggtcaga tgagccgcgt catgagttgc cgcattgcca cgtggctggc gaaggaaaat
     2041 ggccacgccg aggcgctcac aggcgccgtg gcggcgtcag atgcgttttt cccgttccgc
     2101 gacgggccgg acattctcat cgatgccggt gtgactgcct tgattcagcc cggcggaagc
     2161 aagaatgatc agcaggtcat tgatgcctgc aatgagcgca gtgtgccgat gatctttacc
     2221 ggcacgcggc acttcaaaca ctgatgggct gctggcttgg aatctgtata gttctgcgct
     2281 gtgacggcat acgccgttac cggcggtggt acttgtacca cgcggcgcag gtgccctcgc
     2341 tgctgaccat gcacggcccc accgggtgca gcggcgtgca ggctgtgccg aacagggcgc
     2401 agttcgcggg ctcggtcttg ccctgaatga cttcgccgca gcggcacgcg ggatggtcct
     2461 catcttcgcc gagcatcagg ccgaaacgct cgaccgcgtc gaaccggcga taagccggcg
     2521 cgagcccctg cccgctctgc ggaatcacgc cgatcgcgcg ccacgccgtg tcctccggca
     2581 cgaacacgcg ctcgaacagc cccagcgccg tggcgttgcc ctgctccgag actaccgccg
     2641 cgtatacgtt ttccacttct gcccggcctt cggcaacctg ccgcaccagg ttgatcaatc
     2701 cccgcagaat ctgctccggc tcgaagccgg cgatcacgca cggccggcgg tgctgcgcga
     2761 cgatcggctg gtacgctcgc gcgccgatga tcacgcttac gtgcccggga cagagaaagc
     2821 cgtccagcgg cacgtcccca gaagacagca acgccagcat cgccggaatc accagcttgt
     2881 ggcacatcag aacgctgaag ttttcgaggc cgtcgcgcgc ggcctccagt acggcaatcg
     2941 cggtcgccgg agccgtcgtt tcgaagccca cccccagaaa cacgacctgc cgggcaggat
     3001 tgtcgcgtgc cagttgcagc gccgtctttg tggagttcac cacgcgcacg tccgccccgc
     3061 cagcccgcag ccgctccagg ctgccgcgcc gccccggcac acggatcatg tcgccatagg
     3121 tcgcgatggt cacttcgggc cgcgtcgcca gctcaatcgc ggcatcgatg tgccgctgag
     3181 ccgtaacgca gaccgggcag cccggcccgc taaccagccg cagattcggc ggcaacatcg
     3241 agcggacgcc gctgcgaaag atcgagacgg tgtgcgtccc gcagacttcc atgaactgca
     3301 tacgccgccc gacgcgctga cagaggccgc ggagctcttc acacagctca ggtacccttg
     3361 cgctcatccg cgcgctcatc tgccgctctc gtcttgcggg cgattcggcg gtaacacggc
     3421 gggttcaggg ccggccggct ctccgccggc ttcggtcagg gcatcaccca gcgcttggcg
     3481 gaggtagtcc caagtctcgc gtgcgtccgc ctcgctcagg tgcgtgatgg agaacccggc
     3541 gtgaaccagg acccagtcgc cgatgccgac ctccggcgtc agggcggtgc tgatccgcag
     3601 gcggtttccc tgaagatcga cgacggcatc gtcgcccgtt ttctcgatta cttgtgcggg
     3661 cactgccagg cacatcaact gttctccgcg gttttccagg ccgccacgat ggcctgccca
     3721 agcgccaggc caccatcgcc gctgggcaca ctgcgatgat atagcacttt caggccgctg
     3781 gacgcgagca attccacgac ccgcgtcagc agccgccgat tggcgaagca gccgcctgag
     3841 agggccacga cgcgtatgcc atgccgctcg caggcaacgg ccgcagcgtc cgccagcagc
     3901 cgagcgatgg tctcgtgaaa tctcgcggcg attcggggaa ccgcgacatt ggcccgccgc
     3961 tctgcgatga tctcgcgaat catctgcgac atcgacagcc gcagcatccc gccgtgatcg
     4021 cgaacttcgt acccgtacgc ctcaaccgcg ttttccgcat aagccccggc cgcggcttcg
     4081 agagccatgg cggcctcggc ctcgtggcgg tttcgcctgc ataggccgag aagataactg
     4141 acgccgtcga atacgcggcc caggctggag gtcggcgggg cattaactcc cgacgcgagc
     4201 tgtcgctcga agacggccac ttccggagcg gtcggtacgt caccactcgc aatcggtatc
     4261 acgtcggccg ccgagccgcc ggccgcaacg gccagcgcca gcgccggtcg ccaggtatca
     4321 atggcggccg cgtcgccgcc gacaagtggg aaatattcga ggtgccccag ccgctcaaaa
     4381 tcgccccgct cacacagcag cgtttcacag ccccagaccg ccccgtccgt gccgtatcct
     4441 gtaccgtcac acgacaaccc gatcaccggg ccggacacgc caaattccgc catgacgctg
     4501 gccacgtgcg cgtgatggtg ttgaaccgcg agcgtcggga cttcgcgctg ccgcgcgaaa
     4561 accgacgaca gatactgcgg atgcaggtca tgcgcgacca cgtccggctt gaatccgaac
     4621 agctcttcca gccggccgat cgcctggacg aaatgccggt aagcagcggg gttgctcaga
     4681 tcgcccaggt gctcgctgac gatcgcctcg ccgttatgca gcaggcaaac cgttgacttc
     4741 agctccccgc cgactgcgag gattctgcgg ccccgcactg cggccagcgg tccgtcgcca
     4801 tcgaacgctt caagccgaag cggccgcggg gcatagccgc gcgcccgccg gatcggcacc
     4861 gtttcggcgt caaacgtaac cacgaccgaa tcgtcaatcg gccggaaaat ttcgcgatcg
     4921 tgaatgagaa aggcatcggc gacttcgtgc agttcggcga aagcgtcctc gtcgcgaaac
     4981 gtcagtggct ggcccgaaag attcgccgac gtcatgacca acggcccgag tccctcggcg
     5041 aaaagcaggt gatgcaacgg ggcgtacggc agcatgacac cgaaggtatc gacttgttct
     5101 gccacgctgg gggcgatcgt cgggcgagag ggatctaggg atcgagggat cgagcgatcg
     5161 agagaaccct cccacattcc taccttccca ccttcccacc ttccgaggga agggatcgag
     5221 ggatcgagag agcagtattc cggtttcttc cgcgctagaa cgatcggtgc tgccggcgaa
     5281 gtgagtgccg cgatatccgc cggcgtgagg atgcagaacg cctgtgccgc cgctatcgaa
     5341 ggaaccatga gcgccagcgg cttgccgtcg cgcagtttgc gctgtcgcag tgttcgaact
     5401 acgtcctcgc gatccgcccg gcacgccagg tggtatccgc cgatgccttt gaccgcgaga
     5461 atccgccctt ccgccagcat gcgagccgct tcgcggatcg gatcgccctc aagcgcctcg
     5521 accacccacg ccggctcacg ccctcgcgcc gccccctgcg gctttcgcac gcggcacaga
     5581 cgaagctgcg gaccgcacac cgggcaggca tttggctgcg cgtggaaccg ccggtcggcc
     5641 gggtcgtcat actcccgctg acacgctggg cacatcttga agacggccat cgtcgttgcc
     5701 gggcggtcgt acggaaccgt cttgatgatc gaatagcgcg ggccgcaatt ggtgcagttt
     5761 atgaacggat accggtagcg ccggtcagcc gggtcgaaca gctcacgctg gcagtccgca
     5821 caggtagccg catccggcgt aacttccggg cggcgctgcg gatcggcccg cgagccggtg
     5881 attcgaaact cgcgctccgc ttgcaccggc agactcgcgc gccgcagctc tgtaatcttc
     5941 gccagcggcg gcaagcgctg cacgagcgcg gcctcgaaag ccgcaatgca atcagccgac
     6001 ccctccacct cgatgaacgc cccgttgctg tcgttgccga cgtagccgcc caacttgaac
     6061 tcagtcgcca gccgatacac gaagggccga aagcccacgc cctgcacttg cccgcgtaaa
     6121 tccaagcggc ggcgaaccac tttttcttcg ttaaccggca cgcaaacgct ccgcgagatt
     6181 ccgcagcata acaaaatccg gaccaaatta gtacgcgttg caccagaatt tttcagccgc
     6241 cagcctttgg gacggacaaa cctcacgccc gcccgccgcg gcccgcagtt gccctcagcc
     6301 gcgccgccag taggttctgg atcggtttgc tcctgctgtc cagcctccat acgattccgg
     6361 catgtcgagc gattgggaac ccggtcacga tgcggcgctg tcgccggcaa cgcccgatca
     6421 gggaaccgcg tccggcgacg cgcctgcgga attccacctc ccgcatccgg ccgtgcggac
     6481 ggtctggatg ctctccgcaa ctttcacggc ggcggcgctg ggtgtggcag gcctccttct
     6541 cgaagccctc tggctgcagc gcgccacaag ctggccgctt ccgttcggag tgctgaccat
     6601 tgccgtcttc ctcctgtttc tggtgtcggg gctgctgggc gcggacttac gctaccgtgc
     6661 ctggcagtac gccctgcggc acaccgtcgt gctcgcgcac ttcggcgtga tctggaggac
     6721 gcggcgctgt gtcccgcgct cgcgcatcca acacgtcgac atcgagtccg ggccgatcga
     6781 tcgcgcgttc ggattggtca agctcagcat tttcacggcc ggaaccatcg cggccgtcgt
     6841 agtgattcca ggtttgtcgc ccaccgacgc cgagctactc cgcgaacggc tcgttggcgc
     6901 ggagcgcgac catgcctgac tccacgccgg cggaagagag gccggcggag acaggaacgc
     6961 cccggcccca tgtggattca gcgcaaaccg gcgcgggccc ggagatgcgg cgactgcatc
     7021 cagccaccat cttgatcgag ttgctgcggc ggatcggctc gttcgcgtac atcatcctgc
     7081 ttgcgctgct ggcagacttt ctgggcgagc ggcgcgggtc ggattggacc gaggtggcca
     7141 tctcgggcgt gctggtgctc ggtgcagccg gtgcggttct gcggtacgtg tccgtccggt
     7201 ttggcatcga caaccagcgt ttggtcatcc gctcgggcat tctcaatcgc caggtccgca
     7261 ccattccgct cgaccggatt cagaacatcg acttgcgccg cggcgttctg caccgcttac
     7321 tgcgggtggt cgacgtcagc atcgaaacgg ccgccggcgg acgggccgag gctgaactgt
     7381 ctgcgctcag cgaggcggag gcagaacggc tgaaagccga gttgctggag gtccggcaac
     7441 acgccgctgg tattgccccg gaagctcacg aagctcacat gccgctgacc gaggcccacg
     7501 agggcgaggt aatctggcgc gcctcgctat ccgacctgct gctggccggc gcgacggaga
     7561 atcgagccgg attgatcgtc gccggcctgg cgggcctgtg gtacacgttc ggcgcagcat
     7621 tgcagggcag gttcgcagaa caattccagc gcacgtacga aaactggcta ggcgcgggcg
     7681 ggcgcggcac ggcgcttctg atcatcgccg gaattacgct gctgattgtg gccggctggc
     7741 tggtttcaat cgtgctcgcg gtcgtgcgtt actacggctt cgtgctgcgc cgccgcggca
     7801 atgatttgcg gcgccgctat ggtctcttta cgcagttcga aacggtcctt ccgcttggga
     7861 ggattcaatt actgcgcatc gaagcgcctt ggccgcgccg cctgctgcgc tgctgctcgg
     7921 tgtacgtcga aaccgccgct tcggtactcg aggccgaggg cggcggaagc gcggcccttt
     7981 cgccgctggt tcgctggcgg cggctgcccg cgctgtgccg cgaggtcttt ccggagctgg
     8041 acctcgaaca ggtgacttgg aacccggtaa gccggctgac tatccggcgc gggatgattc
     8101 gttacaccgt cgcggggttg atcgtgctcg gcctggcgtc gctctacttg cacggatacg
     8161 cgctatgggg cctgccggtc gtgttcgttc tcgcgctcgg cgcggcctac gcgcgcttcc
     8221 gggcgctcgg atacgccgag cagggccagt tcgtgctcgc ccgcgccggc gtttggacgc
     8281 gccgcatctg ggtcgtgccg cacagtaagg tgcagttcgt ttcagtttcg cagagcccgt
     8341 tccaacggcg gctgaagctg gccaaccttg aagtgcagac cgccggctcg cgcgtgacca
     8401 aagaggcgcg cgtcatcgat ttgccggccg cgaaagccga ggctatgcag gacgctctcg
     8461 cagaccgcgc caacgcgtac ggcgcctggc agccggaagg cgtctgaggc gggtgctgac
     8521 aaacacccgg aatgctgttt gctagaaaaa aagaatgcag gagcggcgag atctccggca
     8581 cctctctcct cctgtttttc gccggcccgc cagctcctgc aactcccacc acgcgcgccg
     8641 caaaagcgcg ttcatgccgc ctgcgatcct cgaaggtcta aaccattctc aaccaaagcc
     8701 gcgactaggt atctggatgc tcctaggctt cgtgccctgc aaagtatacg tgaacgcgca
     8761 cgctgaaagt caatccaaaa ccgcaagaat tccccgcatt ttccggaccc acctcatccg
     8821 aaacctcgac tctcgtggcg tgtgctcacc cgtcccgata ttattggccg gacggcctcg
     8881 cgatcggctt gcgcaagatc atgaagaatt caaccacaaa agaaatcgag cacgtggtcc
     8941 tggcgcatgg gggcggcggc gagctgatgg cccggcttat tcgcgaccac ctcctcccgc
     9001 cattgaagaa cgagcgactg gcgccactta ccgacggggc cgtgctgccg tggacggccg
     9061 gcgatcttgt attcacgacc gattcgtacg tcgtaacgcc cctggaattc cccggcggcg
     9121 acatcggacg cctggcagtc gccggcacgg tcaacgacct ggccgtgatg ggcgccgagc
     9181 ccgtcgccct gagcctgggc ctgatcctcg aagagggcct gccgctcgag gcgttggacc
     9241 ggattctcgc ttcaatcgcc gccaccgccg ccgaagccgg cgtcaccatc gtcacgggtg
     9301 acacaaaagt aatcgagcgc cgcaaaaaac cggccgcgga atcaccgccg gcaggtccgg
     9361 ccgccgacgg cttgttcatc aacaccgcgg gcatcggacg ccgccggccc ggagtgaatc
     9421 tggacatcaa gcgcatcgcc ccgggcgacc tgattctgat caacggcacg ctcgccgatc
     9481 acggcctgac gatcatgtcc gtgcgaaacg gcatcgaatt cgagtcggcc ctgcggaccg
     9541 acgcggcgcc gctaaacggc ttaatcgggc aactgttgga ctgcggcgcg gacgtgaaat
     9601 ttctgcgcga tccgacgcgc ggcggcctgg ccggcgtatt ggccgacatc tgcgaagcca
     9661 ccagtctgag catcgatgta gccgaggatc aggtccccat ttcgcgcacc gcccgccatg
     9721 ccgccgagat gctgggcctc gatccactga ccgtcgccaa tgaaggaaag gtagtggcgg
     9781 taatcgcacc gcagcacgtg gagcaggcgc tccgcgctct tcgcagccat ccgctcggca
     9841 aatccgcggc cgtcattgcc gaagtaaccg gcgccgcgcc gccgctggtc gaactgatca
     9901 cgcgcgtcgg cggccggcga ataattcagc gcccctacgg cgaagacctc ccgcgcatct
     9961 gctaaactga tgattgacta gcgacagaat gcaaaactaa caaaaagcaa aactaacaac
    10021 taataactaa taactaataa cgaagaaccg caacggatac tccaaaggaa cccccacatg
    10081 tccagcacct ccctaactct cctggcgatt gccctcaccg cgctcgccac cgcgcgcctg
    10141 tcattcggcg gcgggcagcc tactactgcc gccacacggc cggccggtga agcagcgctc
    10201 gtaaaaggca acaccgcctt cgccctgggc atttacgacc ggctgcgggc ggaagaaggt
    10261 aacctgttct tctcgccgct cagtatctcc acagcgctgg ccatgacgta cgccggggcg
    10321 cgcggcgcga ctgcggcaga aatggcgcaa gcgctgcact tcgacttgcc gcccgaccaa
    10381 ttgcacaccg cgttcgcggc catcctcaat gaactgaatc caccgcaagg cgccgccgag
    10441 ccgcgaccct acaaactgca catcgcaaac gcactgtggg ccgcgcaagg ccataagttc
    10501 ctaccggcgt ttttgaaaac ggcagaacaa agctaccagg cgggcttggc cgagcttgat
    10561 ttcagccagg ccgccgagca ggcccgccag accattaacg actgggtggc cgagcacacg
    10621 gccggcaaga tcaaagacct cattccgccc ggcgtcctcg acgcgaccac gaagctcata
    10681 ctgacgaacg ccatctattt caaaggcaac tgggccgccc aattcgatcc gaaatccacg
    10741 aaggaagagc cgttcacccg cgctgacggc agccaggtga atgccccgtt gatgcatcag
    10801 acgggcgaat tcggctatct ggagcagccc gatttccagg cgctggattt gccgtatgcg
    10861 ggcaatcgcc tgcgcatgac catcctgctg ccgcgcaaat atgatggact acccgaattg
    10921 gaaaagaagc tgacgccgga ggccctggcc ggctggctcg gccaactcag cgagcagaaa
    10981 gtccaggtcg cgctgccgaa attcaaactg acttcggaat tccgcctgga cgacgcgctg
    11041 cgcgagctgg gaatgcggct cgccttccga gtgggcgcgg ccgacttttc gggcatggac
    11101 gggacgcagg aactgtatat cggcgcggtg ctgcacaagg cgtacgtcga cgtgaatgaa
    11161 gaaggcaccg aagccgccgg cgcgacgggc gtggtcatga agatgcgcgc cacccccggc
    11221 ccgccgccag tcttccgcgc ggatcatccg ttcctcttcc tgatccgcga tgtgcaatcc
    11281 ggcagcatct tattcctggg aagactcgca aacccctagc cgaagccgcg ccgtacccgc
    11341 cgcgcaaatt acgaccccgc gcgccaagga gcgaaaacgt gattatttga cgacattccg
    11401 gggcgatacg ccccggctct gctcgtcgcg aaggcgcggt ttgttcgttg cgtttcacac
    11461 gctcctcgct tacgttgccc caactctacg gagcttatta gggagcgacc atgagcacgc
    11521 aaaccagcat cagtcccgaa ggcgaaaagt tcgccctacc cacccctgag caataccccg
    11581 ccgagttcgc ccggctgaaa aaactcgtcg accagaaccg cgccgaagga cgcgaaatcg
    11641 tggtcgtggt cggcgtcggc ttcgtcggcg ccgtgatggc cgcagtcgtc gccgacagcc
    11701 gcgacaaggc caccggcaag cacaacaaat tcgtgatcgg catgcagcgc cccagcccgc
    11761 gcagctattg gaaaatcgag ttgctcaacc gcggcctctc ccccgttaag gccgaagatc
    11821 ccgaggtcga ccagctcatc ggccgctgcg tcaaggaaac caagacgctc tgcgccacgt
    11881 acacgcacga ggttatgaag ctcgccgacg tggtcgtcgt cgacgtgcag tgcgattacg
    11941 tcaagcgcga cctcggcaac ctgcgcaccg ggaccgccga catggccgca ctcgaaaaga
    12001 gcctcgccac catcggcgaa atgatcccgc cgaactgcct ggtgctcatc gaaaccaccg
    12061 tcgcccccgg cacgaccgaa tacgtcgcct acccgattct caagaaggct ttcgaaaaac
    12121 gcggcatcga aagcgagccg ctgctggcac acagcttcga gcgcgtcatg cccggccgtc
    12181 attacgtcgc cagcatccgc gacttctggc gcgtctgtgc cggtgtgaac gaggaaagcc
    12241 gccggcgcgt ggtcaagttc ctcacggaag tgctcaatac cgagaagttc ccgctcaccg
    12301 tcatggatcg cccgcttgaa agcgagacga ccaagatcgt cgagaacagc taccgcgcga
    12361 cgatactcgc cttcctgcac gaatggagcc ttttcgccga acgcaacggc gtcgacctga
    12421 tcaaggtcgt caacgccatc aagatgcgcc ccacgcatag caacatgatc ttccccggcc
    12481 ccggcatcgg cggttactgc ctgcccaagg acggcggcct gggcatgtgg gcgtataagc
    12541 acctgatggg ctttgaggat gacatcttca agctgaccga ggcggctatc aacatcaacg
    12601 acacgcgcgc cttgcacgtc gcagagcttg tccgtgacgc cctgcggaac atgggcaagt
    12661 atttggcagc ttcgaagtgt gtactgctcg gcgccgccta tcgcgaagac gtcggtgaca
    12721 cgcgctatag cggcagcgaa ctggtcgtgc gcaagttgac ggaaatgggc gcagaagtcg
    12781 cgatccacga tccgtacgtg cagcactggt gggaattgga ggcgcaggac acctacccgg
    12841 cccccggcca aagctgggcg cgattcttcc gcaatcagga gcacctgaag gattcccacg
    12901 ttgactcgga catggccacc gccctcaaag acgcggatgc ggtgatcctg gccgtccgcc
    12961 acgaacccta cctgcgcctg agcccggacg aggtcgtcaa aatggccggc aagccgctgg
    13021 cggtcatcga ctgtttcggc atcctcgacg acgccaaaat ccgtcgcttc ttcgagctgg
    13081 gctgtgaagt aaaaggcctc ggccgcgggc acgtgaagcg catcaaggac agcgttcgcg
    13141 gcagctaaaa gccacggcgc aaatagcgtg cgtgtgccac tgcttgaaag cgcagcttca
    13201 agcagtgcgg aggtcccgcg atcacggttc cgctaagccg gaggacgcca caatagcgcc
    13261 accccgtggc gactctttac cgtgcgctat tacgccagca actccgccag cttcttcttc
    13321 gactccgcct cgaccttctc gtgcaggctc tgcccatgcg agtccatcgt caccacgcat
    13381 gggaagtcct cgacgcgaat ctcccagaac gcctccggca cgccgaagtc gagcttgtag
    13441 acgttcgtga cttctttcac gctttcagcg atgagcgtcg ccgcaccgcc gatcgcgtgc
    13501 aaatacaccg ccccgagctc gcggcacgcc gccaatgtct tcggccccat gccgcccttg
    13561 ccgatgatcg cccgcacgcc gaagtgccgc atcacgtccg cttcgtacgg ctcttcgcga
    13621 atgctggtcg tcgggcccgc cgcaacgaat tcgtacttgc cgtccgggcg cttccgcact
    13681 accggcccgc aatgatagat gacgccgccg gccagcccgc gcttcagcac gtcatagacc
    13741 ccctgctcct ccgccgggca cgggccgacg atgaacttct ccttcatgta cttatgcgcc
    13801 gcgtcacgcc cggtgtagat gacgcccgac agcaacacct ggtcgccgat gcgcagttcc
    13861 cgaattcgtg attcggctag cggaatgttc agtttggtag ccattgtcct actcctctcc
    13921 gcgcggctcg attgtcgcct ggccgtcccg gatcgtcagc gtcttccgcc ggtccgccca
    13981 acacatgtac gcaaccgaca caaaaaagca cgccggcaga cggtggagct gcccggcctt
    14041 caccccgagc accgtggtct tgcccccgaa acccattgga ccgatgccca actgattgac
    14101 ctcgtcccgc agcgtctttt cgaatgcatc cagctccgga tccgggttct catcctccag
    14161 cggccggaag agctgctcct tcgccttctg catgctcgtc acccggtcgc cgccgacgcc
    14221 cacgcccagc acacccggcg cgcagccaaa accctgggcc ttgaacgcgg cgtcgagcac
    14281 cgccttccgc acgcccgcaa tgtcgcgccc cgcctggaac tccggcgacg gcacgctgta
    14341 ttggcagccg caattctccg acccgccgcc cttgagcagc agcgagatgc gcaggtaatc
    14401 gtgctcccac tcctcgaagt gaatcgtggg aaaatcaacg cccgtgttgt cgccgctgtt
    14461 cttgcccgac agcgccgcca ccgcgttcgg ccgcagatag gcccgctccg ttgcaatgcg
    14521 gactgcattg cgaatctgct cgcctaacgc gcgctgcgaa acgccgaccg ggtgccacac
    14581 gtaaaaaatc ggcgtccccg tatcctggca aatcggccgg cccgtctcgc gcgacagccg
    14641 cacgttctcg aggattgtct ccagcgccgc ctgcgccgcc gaaccgtcag cctctttctg
    14701 acaggcgttg cgcaaggcat gctcgacatc cggcgagaga tcggtagagg tcgcgcgcac
    14761 cagttgaacg agatgttcgg tgatatcttg catagctcgc ctcactaagt gaaaaaacgg
    14821 gccgtttccg acccgtttaa ctcattattc agcatgccga cgggcgggtc aaccggttgt
    14881 ctggcaagcc gcgcagtccc ccttggcgag actcacgggc ctgtcttggg tgaaaaaact
    14941 ctcgaactcc attttgcagc gctggcaact cgcgttcggg gcggcgataa tgcccctgac
    15001 gccgtaatgg actgcggacg ggggcgctat atgcgcggcc gcggtgcggg actgttccag
    15061 cgctgcacag ccgtgcccgg cgacgaggta gtagggagat gctttacgcc gccctgacat
    15121 tctggctgct cgtaatcatg ttctccgctt ggggcgtaca ccacatttgg gccgggctca
    15181 tcaaaccccg tagcgtcaac ttcatcctgc tgcccggcac actggtggcc caactgggcc
    15241 acgtcatggg actcctggtg accggcaact cggtaaagaa cactcagctt atgggggatg
    15301 acgaaaaagg tgatccgaag tcggccaacc ccgatcgtgt tcgcctgccg gtcgtaggac
    15361 cgataatcgt tggtcttctg ccgctcgtgg cctgcgccgt ctgcctttgg ttcgccgccc
    15421 gctactgggg ctggtcggtg atccaaggct tgaacgccga aggcgtccgc ggcgggctgg
    15481 cgcgagaact gcccaccagc ctgcccgcct tctgggaatt cctgcgcggc gcgatcagcc
    15541 tgatggagcg catgctggac ggcgtcctgc gaagcaacct gttcaactgg tcgaccattt
    15601 tgttccttta tctggcgatc tgcctgacgg ttcgcatggc cccgttcgaa ggcaaccgcc
    15661 gcggcgccat tggagcggtg ttgttggccg gcctggtcat cggcgtgctg ggctcgctca
    15721 tcccgtcggt tcaggaaact ttggcggctt ccggcccgag ttggctcatt ctcagttttg
    15781 cggtggcggc cctgctcttc atgctaatga tttcgctgat ggcgactggc gtagtcgggc
    15841 tggtccgcat tctggcgggc aaacaggagg ccggctctgc agcgcggaaa ggctagtgcg
    15901 atcccccgcc tgcggccgat aataaataag tggcgcctgg gccacgcgac cctggaaacg
    15961 acatgccgca tccggtattg acgcatcgca tagattagta tgggagctgt agggccttag
    16021 cgcgcgcgac cagcgcagcg acaacatccc cacgagcagg agtttctcat gccgaacccg
    16081 tttcgcgctt acggcaacgc gccggctttc ttcagactgg cttcgattta cacggtcgtg
    16141 atggcggtcc tcatctacgg cggctgccca aaacccccga cgcccatcac caattccaac
    16201 gacaacgctt cgagcaacgc aaacagcaac agctcgaaca gcaacagctc tccgaacaac
    16261 aacggcgccg tcgcaaacaa gaacggttcg gttgacaacc tgaacatcaa cgtcaacaac
    16321 tccaacgaca acgacgtgtt cgaccccgag aagcccaagc cggacacaat cacgttcgaa
    16381 gaaattgtca gaaccggcga cttcgtgccc gacgtcaacg gcaccgcaac cggcgcagtc
    16441 ttctcagatt ttggcagccc gattatcgac gccagtgggc gcgtcgcgtt ctgggcccgc
    16501 tatacgggcg gatccggcca tggcgggctg tatgtctgga ttcccgcaaa ggccggcgtg
    16561 ggcgggacgc tcaaagcagt gatagacgac tccgggaaca tccccgtacc cagcgccaca
    16621 cccggcaagc ccacggccga aaacttcatc tgcaacccgg cgacactcga tattcgtacg
    16681 cagccattga cctggagcgg tgacggacgc ctgctgttcg tgtcggccta cagcggacca
    16741 ggaaacaagc tcatggtacg ttggtctgcg caacaggctg accccgcaaa ggcgctgcta
    16801 cgtgtggccg atacacaagc cattgccgac gccatgggca ctacggtcgc agctaccatt
    16861 gtgacttttg ccaatattgg ttgctcggac gaggggcttg tattcttcca gacgcactac
    16921 tttgacatat cagccaacaa gtccggcgac gcgatatgca aagcagaggt gagacaggtc
    16981 ggcggcacga ccaaattagt tgtcaccccg gtcgtgcccg tggccagcgc caccccgtcg
    17041 gccagcgacc gcggcgcggt tccggaacaa ggcccgtcgg cagccttcgt cctgtacgac
    17101 cactacacga ctctgagtcc tgacggctac ctgctgttcc agggcgccta cgacagcggt
    17161 acagggacgc acggcgccta tctgcgcatt cccggcgacc tggtcgtgcg tgtgatcgac
    17221 agccggcctg actccgaatg gctcggcttg gacccgggtg tggtcatcgg caacgatgag
    17281 gatttttacg aagccatcgc catcagtaca aacgtgcaca tcgccattca aacgccgatc
    17341 atcgccggcg gttcatcagt caggcgcgtg atcctgtggg acttcgccct gggtgagtgg
    17401 accgtcctgg tcaccgatga agacagggcg atcaccgact tgttgtcggg tgtcagcgac
    17461 ggcggagcgc tgctaggctt ggccgggtcc gagccgtata tcgttggcgt gggggcggcg
    17521 gtcgatatcg cggccaattt gtcttctgaa ctcgacggcg tcgccttgga gtggatgggc
    17581 tggggcgcaa cctccggctc cattaacaac tccggccacg ccatcgtgcc ctacgtccgt
    17641 gtcgccgaat caacccaggg tctcggcttc tggaacggtg aggaattact gatcatcgcg
    17701 gatcagcttc tgaacatccc ggacgccgaa gccgcaatca cagagataac cgctgagtcg
    17761 atgcccgaaa ccgaccgccc cgggcgctcg ggaattttga acgacaacga cgaggcggtt
    17821 ttccggctcg atcttggcac
//
LOCUS       DAOSYS010000046        17775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2629_length_17775_cov_4.834312, whole genome shotgun sequence.
ACCESSION   DAOSYS010000046 DAOSYS010000000
VERSION     DAOSYS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17775
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_17775_cov_4.834312"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLU99_04465"
     CDS             <1..191
                     /locus_tag="PLU99_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16343.1"
                     /translation="RSTPDVHQVRLLLGLIYSRYLQQWQRAAEHLQSALENLTQPEQR
                     ALAESELRHVQAQVQAEA"
     gene            complement(208..3216)
                     /gene="uvrA"
                     /locus_tag="PLU99_04470"
     CDS             complement(208..3216)
                     /gene="uvrA"
                     /locus_tag="PLU99_04470"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOO16344.1"
                     /translation="MASEPKYIRIVGASQHNLQNVTVELPRERLIVLTGLSGSGKSSL
                     AFDTIYAEGQRKYVESLSVHARHFLEQLSKPEVEYIEGLPPTLAIEQRAAAANPRSTV
                     ATTTEIHDFLRVLFARVGIPHCPKCGARIHAHTVDQIVDAVLSYPPETRIMLLAPLVR
                     RQAGDLHAVLRRIQREGFVRARVNGELRDVKDLSELPKGRKFDIDVVVDRLVLRPEIR
                     PRLADAIELSFSLSDGVVIVSRAADAARRDGRPPLETPANGTTPVNGASSAAAVSNHG
                     KGPRAAQTNGWLDETFSERYACTRCGITLPELSPRAFSFNSPYGACQTCGGLGTTLRF
                     DEQLVIPNDNLALANAIAPWATAQRDVAASQRAALRQLCAAFDVKPETPFKNLPDDLR
                     RIVLYGTRPTDAKRFGIQFEGVIPNLERRTKAAGSDGGRSRFINYQSEHVCESCHGAR
                     LRPEPLAVRIGGRNIHQLVQLPIAQATRLFDEMSFTGEAAVIAEPILREVRQRLRFMD
                     AVGLGYLTLDRPSVTLSGGEAQRIRLATQLGSGLVGVCYVLDEPTIGLHQRDNERLLR
                     SIRNLVDIGNTAIVVEHDEDVIRAADYLVDMGPGAGLHGGRIIAAGEPQTVLNDPNSI
                     TGKYIRGEYSIPLPEERRTPKSTDFLEVVGAAENNLKRINVRFPLGVFCAITGVSGSG
                     KSTLVSQILLPALRRRLYNSRERPGAHERLIGASRIDRVIEIDQNPIGRTPRSNPATY
                     TGVFDEIRRLYAKTKEARVRGYNASRFSFNAKGGRCEACQGQGVRRIEMHFLPDVFVE
                     CQECKGTRYSRETLDIRYRGKSIADVLELRVEENLRFFESFPKIRQGLQALSDVGLGY
                     VRLGQPSNTLSGGEAQRVKLAAELSKGVLGPVVRNGPPSANAAQRARQESHTLYVLDE
                     PTTGLHFADIQTLLAVLNRLVDMGNTVVVIEHNLDVVKTADWVIDLGPEGGEAGGQIV
                     AQGPPEEIAACPKSHTGRYLTRKLQSAPQP"
     gene            complement(3427..4896)
                     /locus_tag="PLU99_04475"
     CDS             complement(3427..4896)
                     /locus_tag="PLU99_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16345.1"
                     /translation="MPSSETATALQRGPTGLVARVRDFLNAFRDLLHLPRAFWLINLF
                     FMIDSMAYFGILTLMQEYLHLDVKLADWVAGFFISFFTGAVTVFMIGFGGVGEKLGWC
                     RGLALAITLCLVGRIGYCLAPGVGFAAALALVLAGLVLIALGEGMVQPVCYAGIKQFT
                     DEKTSAMGYGLIYAIMNFGIFLIGFVSPLIRVPVEKVHEATALAQPAPGTIGRFFADR
                     GVSGLNAVNWTCTAITLVALLLLLAFMTMGANQTGQRSRQSQPSPPSGHAGQAPWCAR
                     VGRYLSEGPFSNARFVFFIFMLLPVQTLFAHQWLTMPAYVLRAYSEGVANRMEWIVNW
                     INPGIIFFGVPLITALTRRANVYTLMIVGSAVSALPTFLLAFGPHTPLLIVYLFLFSI
                     GEALWSPRFLQYAAELAPEGKLGQYMGLANVPWIMAKSTTGLYSGYFLGRYCPTEGPQ
                     DTQTMWLIYSLIAIASPIGLLLGRRWVMRGSLGAPQAAV"
     gene            complement(4909..6501)
                     /locus_tag="PLU99_04480"
     CDS             complement(4909..6501)
                     /locus_tag="PLU99_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16346.1"
                     /translation="MATQVTEETVVKKSPLQEIVQPFIDLAHAPRALWGINLAYVLEG
                     MVYFGVLSYLAKHFSDFIFAGMAHADVHSHNMVMVLTAGITISMFFLGFVADKWGVRR
                     ALIAALSLMLVGRALISAAPTVLNLQPSGLWSQLHLVTMGGILLVVIGYGMYQPAAYA
                     GVRQITTPKTAGMAFAMLYALMNLGGWLPTFAFLLRDDDYLGMGIPGVYWVYTGFTLI
                     ALLSTVLILSARTFKQAREAALAETAALNAAEQKASAAAGPTKAQPDVKPEELVVDAR
                     IPWHMWLVLLGILVAIYFRAPEAWRYQIMAVVFVAAIAAAIIPKVRSAVAQHPLANSK
                     FFFFIFALMPVQTLFTYNWLILPQYISRAYEGWIGRYFEIASNANPILIFILVPIIAA
                     ATQKAKVYNMMVIGTLIMAAPAFLLAIGPYPLTLAGYILIMTIGEAMWQPRFLQYAAE
                     IAPEGRTGQYMGVAQLPWFLTKMLVPLLYSGIMLEKFCPADGVRDTRTMWLIFACIAC
                     TTPVLLLLAKGWVGRDFKTKAA"
     gene            complement(6871..7824)
                     /locus_tag="PLU99_04485"
     CDS             complement(6871..7824)
                     /locus_tag="PLU99_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOO16347.1"
                     /translation="MVSRVPRRPEPEQPRPVDIEEPPPGEAPVRPLWSGTISFGLVSV
                     PVNMYPANRAGGVSLRMLDQDGTPLKRRFYCPRENREIHPEHIIRGYALESGEYVVVR
                     DEELEALEPKKSRDIDLRLFVDAKDIDPMYFERAYFLAPASDSNKAYRLLASTMERLG
                     QAGVATFVMRGKEYLVAIFSEGGILRAQTLRFADEIRSPEDVGLPQNVEASPAERTRF
                     EREIQKLAGKRFDPAELQDEYAEGFRKLIERKRRERRDVISAPETAEAAGPEPDLIDL
                     IRRSMRGGGSKAARSKGNRNGNGRRGKRAEGKRSAGRSSRR"
     gene            complement(7855..8643)
                     /locus_tag="PLU99_04490"
     CDS             complement(7855..8643)
                     /locus_tag="PLU99_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOO16348.1"
                     /translation="MAARAIWKGVLKFGSAAVPVKVYSAVQDRDIHFHLLHDQDQVRL
                     EQRMVNPVAGRTVGSDEIRKGFEAEPGLFVLLEDEELAELVPESSRDIEITHFLPAGA
                     INNQWYDRPYYLGPDGNAGQYFALAEALAKTNREGLARWVMRKRSYVGALRAEKGYLV
                     LNTLRHADEVVPVSELEPPQGRALDKKEQAMAEQLVSVFRGKFDPEQYRDEYRQRVLK
                     LVESKARGERIRLEKPKPKKAPAKPLADLLAASVKAARKEAAHV"
     gene            complement(9798..10538)
                     /locus_tag="PLU99_04495"
     CDS             complement(9798..10538)
                     /locus_tag="PLU99_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16349.1"
                     /translation="MPVERITWGELEEYFTTYLKRDFSTAARLRAGLRRRIRRLLPRS
                     RNARTEAQGWLLLADLYLRAPQTERCVRKALALAPRNPEAHAELQLRWDLSYGMCLAR
                     LAETFGITWECGWGVSGYARHVANCLTFGRCALDADAVAHYCRTAREGKALRLPELFK
                     CETPPEDYFGSGAYHEACLLFVAYLWQISATNAEPWAAFRRFLLAKTMEPGMFITDLE
                     KAFRSPMEQIECDFATWVRQHSEHEHAG"
     gene            complement(11053..11502)
                     /locus_tag="PLU99_04500"
     CDS             complement(11053..11502)
                     /locus_tag="PLU99_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOO16350.1"
                     /translation="MGALNLPPSGVVYLDANCIIYAVEKVAPYDTALAPLWQAAASGQ
                     FSVISSELVVLETLVKPLRDANARLEAAFRAFLFDSTEFHLAPLELRILERAARLRAE
                     LGLKTPDAIHAATALDAAAALFITNDPCFRRVPGLPVTVVSETVTAQ"
     gene            complement(11490..11729)
                     /locus_tag="PLU99_04505"
     CDS             complement(11490..11729)
                     /locus_tag="PLU99_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16351.1"
                     /translation="MGAARKINTTVQAGGKIEVRAPDLPPGQPVEVTIRLVHEQPNGR
                     RSVLDILAECPGGLLFKTPADVDAYIRQERQSWER"
     gene            12318..12818
                     /locus_tag="PLU99_04510"
     CDS             12318..12818
                     /locus_tag="PLU99_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16352.1"
                     /translation="MKSGSQWAETMFLVGTYELVIDAKNRLSVPFNIRRKLDEHSFYA
                     LPGGRPRTLALYPESYFEKLRPVPPVESLSDTTQAWRQFEYSQCALLETDNQGRILLP
                     DRLLKRAGIEKEVTLIGVQDHLELWNRRDFEEFENSQWEHLAENRARALQELRAFAPQ
                     PSGLQA"
     gene            13164..14102
                     /gene="rsmH"
                     /locus_tag="PLU99_04515"
     CDS             13164..14102
                     /gene="rsmH"
                     /locus_tag="PLU99_04515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOO16353.1"
                     /translation="MTVSRVATGQQIVADLRREHQPVLVEPLLDLLKLEPGQVVLDGT
                     VGLGGHAAVFAPRIMPGGRYIGLDLDEELLVEARQRLSGLAGHGLDLFKANYAEYPEV
                     LAALGLQRVDVLFLDLGVNSAQLADPARGFSFESEGPLDMRYDRTQADKALDLVNAAS
                     EDQLADLFYELGQEPLSRKIARRICQVRHSGRITTTRVLASAVESAYAAAGKGPSRRS
                     PATRVFQALRIAVNHELENLQRFLDLAPTHLAPGGKLAVISFHSLEDGIVKRWLRGQD
                     DMEEITRRPVVADPAERHSNPRSRSAKLRVARRMEV"
     gene            14123..15274
                     /locus_tag="PLU99_04520"
     CDS             14123..15274
                     /locus_tag="PLU99_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOO16354.1"
                     /translation="MALNFKAALGICLFFIAGLCYVVNNVALPSQPSISPAATVGGAA
                     LPGERSNFAAGRLERKNPVESQRLAGRRARAVAAAEGAPPETRTVVLPPIVRQETPSF
                     PAALAAQPSVPTPEAHAGEAVVALAPAGALLRTRIVEETDQSAWEETPAQARPVTLTA
                     IEAPEPAAPSNPPAAEPVAVAAAAGTYKVRKGDSLTRIAQRVFGSSEQRHQQMLIDAN
                     PKIAKRVNKLYVGEVLTIPSIPGVAAASERASGQPSGTVAVKQAESDGKKESKPATVA
                     AVAASTTKKKSASADNSGKGTKRTTVAAAQRPSRSGKKGRKASSGGETATERWYTIRG
                     SDSLASIARDVLKDSRRWRELADVNGLRDPNKIIPGMRLKLPPAGPRQG"
     gene            15318..15599
                     /locus_tag="PLU99_04525"
     CDS             15318..15599
                     /locus_tag="PLU99_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16355.1"
                     /translation="MTLTRIIVVLVGALAVMMTVIILRSESTRQQYEISVLEGREDVL
                     RQEFNREQLALQRLRNPADLLERVQKIRLAAPQPTGPPPAAQRRRSTGP"
     gene            15620..17431
                     /locus_tag="PLU99_04530"
     CDS             15620..17431
                     /locus_tag="PLU99_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOO16356.1"
                     /translation="MRSAAWLFLLVAALLAAGAGRLAYIQYAEGDALRERAERQHTAS
                     VVIPAQRGDILDAQGRLLAGSESWPSVYADPSLLQDPRDRKFAAYSIAPVLGLAAEDV
                     DALLRDRAESRFVWLKRHVSPEELAAFHKLRNARRLYALDVQYEPARIYPHGRLASHV
                     LGFVGAPEEGVRHGLAGIELSQDKYLIGTPGHRTRTVDARRRPLCSHVDGFVPPQDGA
                     TVILTIDAYLQQQTQTHLKNAVETFKAAWGTAVLMDPQTGEVLAMATFPDFDPSDPLP
                     DDTKAGEPDERLRNRAVSDAYEPGSIFKPFVASCALEENITRLDEVFAINGPTHNFGR
                     RIIHDTHAYGSLPLHEVISRSSNIGMAMLGTRCENARLYRFLRLFGFGDRTGIELPGE
                     HTGMVLDFSRWTSYSTQSVPIGQEVSVTAIQVATAFSVFANGGILFRPRIVRGVVGPD
                     GEPVWDNSRPIPIRRVLSREVAERFREEALIEVVMSKIGTGKRARIPNYRVFGKTGTA
                     QIAHAGGGGYMGGKYTGSFVGGAPGDKPRVVAIVSLSAPSSGQYYGGTVSAPAVGEIL
                     ADALAYMQVPPEVTSADENGIIPGRKAGSGVQDAGGW"
     gene            17457..>17775
                     /locus_tag="PLU99_04535"
     CDS             17457..>17775
                     /locus_tag="PLU99_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOO16357.1"
                     /translation="MRKLAELLNGIVDQGAYAPHGDRIVTGLFDDSRLVQPNSVFVAV
                     SGTATDGRRFVPDAVQRGAGVVIGEGLPSLNGAVTVNVPDVRAALALLAVRWYGLDDG
                     PARD"
BASE COUNT         3220 a         5500 c         5887 g         3168 t
ORIGIN      
        1 atcgctcaac gccggacgtg caccaggtgc ggctgctatt ggggctgatc tattcgcgtt
       61 atctgcagca atggcagcga gcggcggagc acctgcaatc ggccctggaa aatctgacgc
      121 agcccgagca gcgtgccttg gcggagtccg agctgcggca cgtgcaggcg caagtgcagg
      181 ctgaagctta gcggcgaagc gggactttca tggctggggg gcggattgca gcttgcgggt
      241 caggtagcgc ccggtgtggc tcttcgggca agcggcgatt tcttctggag ggccttgggc
      301 cacgatttgg ccgccggctt cgccgccttc ggggccgagg tcgataaccc agtcggcagt
      361 cttgaccacg tccaggttgt gctcgattac cacgacggtg ttgcccatgt cgaccaagcg
      421 gttcagcacg gctagcagcg tctgaatatc ggcgaaatgc aggccggtgg tcggctcgtc
      481 gagcacgtaa agcgtgtgag attcctggcg ggcgcgctga gcggcgttgg cgctcggtgg
      541 gccgttgcgc acgacgggcc cgaggacgcc ctttgaaagc tcggccgcca gtttcacgcg
      601 ctgggcttcg ccgccggaga gcgtgtttga gggttgcccg aggcggacgt agcccagccc
      661 gacgtcggac agggcctgca aaccttgcct gattttcggg aagctctcga agaagcgtaa
      721 attctcttcg acccgcagct cgagcacgtc ggcgatggat ttgccgcgat agcgaatgtc
      781 gagcgtctcg cggctatagc gcgtgccctt gcattcctgg cattcaacga acacgtcggg
      841 caaaaagtgc atctcgatcc gccgcacgcc ctggccctga caggcttcgc agcggccgcc
      901 cttggcgttg aagctgaaac ggctggcgtt gtagccgcgc acgcgggctt ccttggtctt
      961 ggcgtacagg cggcggatct cgtcgaagac gccggtgtac gtggccggat tgctgcgcgg
     1021 cgtgcggccg atcgggttct ggtcgatctc gattacgcgg tcgatgcggc tggcgccgat
     1081 caggcgttcg tgggcgccgg gccgctcgcg cgagttgtac aggcggcgcc gcagcgcggg
     1141 cagcagaatc tgggaaacca gcgtgctctt gccgctcccg ctgacgccgg tgatcgcgca
     1201 gaacacgccc aacggaaagc gcacgtttat tcgtttgagg ttgttttcgg ccgcgccgac
     1261 gacttcgagg aaatccgtgg atttgggcgt gcgccgctct tcgggtagcg ggatcgaata
     1321 ttcgccgcgg atgtatttgc cggtgatcga gttaggatcg ttcagcacgg tctgcggctc
     1381 gccggcggcg atgatgcggc cgccgtggag cccggcgccg ggccccatgt cgaccaggta
     1441 gtcggcggcg cggatcacgt cttcatcgtg ctcgacgacg atggccgtgt tgccgatatc
     1501 gacgaggttg cggatggagc gcagcaggcg ttcgttgtcg cgctggtgca gaccgatcgt
     1561 cggctcatcg aggacgtaac atacgccgac caaaccgctt ccgagctgcg tggccaggcg
     1621 gatgcgctgc gcctcgccgc cggagagggt gacgctgggg cggtcgagcg taaggtagcc
     1681 caggccgacc gcgtccatga accgcaggcg ctgccggacc tcgcggagga tgggctcggc
     1741 tatcacggcg gcctcgccgg tgaagctcat ctcgtcgaac agtctcgtcg cctgggcgat
     1801 cggcaactgc accagttggt ggatgttccg gccgccgatg cgcaccgcca gcggttcggg
     1861 ccgcagacgc gccccgtggc aactctcgca tacgtgctcg gactggtagt tgataaagcg
     1921 gctgcggccg ccgtcggagc cggcggcttt ggtccggcgt tcgagattgg ggatgacgcc
     1981 ctcaaactgg ataccgaagc gtttggcgtc cgtcgggcgc gtcccgtaga gcacgatgcg
     2041 gcggaggtcg tccggcaaat tcttaaaggg ggtttcgggt ttcacgtcga aggcggcaca
     2101 aagctggcgc agggcggcgc gctggctggc ggcgacgtcg cgctgcgcgg tggcccaggg
     2161 ggcgatggcg ttggccagcg cgaggttgtc gttcgggatg acgagctgtt cgtcaaagcg
     2221 cagcgtggtg cccagaccgc cgcaggtttg gcatgcgcca tagggcgagt tgaagctgaa
     2281 ggcgcgcggc gagagctcgg gcaaggttat gccgcaacgg gtgcaggcgt agcgctcgct
     2341 gaaggtttcg tcgagccagc cgttggtctg ggcggcgcgc ggccctttcc cgtggttcga
     2401 aactgccgcc gcggaggacg caccatttac gggcgtagtg ccgttcgccg gcgtttcgag
     2461 cggggggcgg ccgtcgcgcc gcgcggcatc cgcggcgcgg ctgacgatca cgacgccgtc
     2521 actgagcgag aaggacagtt cgatcgcgtc ggcgaggcgc gggcggattt cgggccgcag
     2581 cacgagccgg tcgaccacga cgtcgatgtc gaatttacgg cccttcggca gttccgataa
     2641 gtccttcacg tcccgcaatt cgccgtttac ccgggcccgc acgaaccctt cgcgctggat
     2701 gcgccgcagg acggcatgca gatcgccggc ttggcggcgg acgagcgggg ccagcagcat
     2761 gatgcgcgtt tcgggcggat agctgaggac cgcgtccacg atctggtcga cggtgtgggc
     2821 gtggatgcgg gcgccgcact tggggcagtg tggtatgccg acccgggcga acagcacgcg
     2881 cagaaagtcg tgaatttcgg tcgtggtcgc cacggtggag cgcgggttcg ccgccgcggc
     2941 ccgctgctcg atggccagcg tcggcggcag gccctcgatg tactcgacct cgggcttgct
     3001 gagctgctcg aggaagtggc gggcgtggac gctgagcgat tcgacgtact tgcgctggcc
     3061 ctcggcgtag atggtgtcga acgccaggct gctcttaccc gagccggaga ggccggtcag
     3121 gacgatcagg cgttcgcgtg gcagctcgac ggtgacgttc tgcaggttgt gctgggaagc
     3181 gccaacgatg cggatgtatt tgggttcgga ggccactgca cgtctcaggc ggtgttaccc
     3241 gatccgcgcc gcccagtggg ccctgcgccg gcagccgcgg aacgaaccac gcattgtact
     3301 ggaggatgct acgagtttcg agcggacgaa gtgccgccgg tgtcttctat cttccgataa
     3361 tgtatgcggt aacgtggcgg atccgggcgc gcgttaagtt cttcacccgc cgcgacagcc
     3421 agaggctcaa acggcggcct gcggcgcgcc taaactgccg cgcattaccc agcggcggcc
     3481 gagcaacaag ccgatcgggc tggcgatcgc aatcaggctg taaatgagcc acatggtttg
     3541 ggtgtcctgc ggaccctccg tcgggcagta gcgtcccaga aagtagcccg agtacaaacc
     3601 tgtggtggac ttagccataa tccagggcac gttggccaag cccatgtatt ggccgagctt
     3661 gccctcgggc gccagctccg ccgcatattg caggaaacgc ggcgaccaca gggcctcgcc
     3721 gatcgagaac aggaataggt acacgatcag cagcggcgtg tgcgggccga acgccagcag
     3781 aaaagtgggc agggcggata ccgcgctccc gacgatcatc agcgtgtaga cgttggcgcg
     3841 acgcgtgagt gccgtgatga gcgggacgcc gaagaaaatg attcccggat tgatccagtt
     3901 gacgatccac tccatgcggt tggcgacgcc ctcggaatag gcgcgcagca cgtaggccgg
     3961 catcgtgagc cactggtgag cgaatagcgt ctgcaccggc agtagcatga agatgaagaa
     4021 cacgaagcgt gcgttgctga acgggccctc gctcaggtac ctgccgactc gtgcgcacca
     4081 gggagcctgc ccggcatggc cggagggggg actaggctgg ctttgcctgc tccgttgccc
     4141 cgtttggttg gcccccatgg tcatgaatgc cagaagcagc agcaaggcca cgagcgtaat
     4201 cgcggtgcag gtccagttca cggcgttcag gccgctgacg ccccggtccg caaagaagcg
     4261 cccgatcgtg cccggggcgg gctgtgccaa cgcagtcgcc tcatggactt tctcgaccgg
     4321 aacgcgaatg agcggcgaca cgaagccgat cagaaagatg ccaaagttca tgatggcgta
     4381 gatcaggcca tagcccatcg cgctggtttt ctcgtcagtg aactgcttga tgccggcata
     4441 gcagaccggc tgcaccatgc cctcgccgag ggcaatcagc accaacccgg ccaagaccag
     4501 cgccaaagcg gctgcgaaac caacgcccgg cgccaggcaa tagccgatgc gccccaccag
     4561 gcacagcgtg atcgccaaag ccaagccgcg gcaccagccg agcttttcac ccacgccgcc
     4621 gaagccgatc atgaagacgg tcaccgcgcc tgtgaagaag ctgatgaaaa atccggccac
     4681 ccagtcggcc agcttcacgt ccaggtgcaa gtattcctgc atgagcgtca ggatgccgaa
     4741 gtaggccatc gagtcgatca tgaagaacag gttgatcaac cagaaggcgc gcggcaggtg
     4801 cagcaggtca cggaaggcgt tcaggaaatc cctgacccgc gccacaaggc ccgtcggccc
     4861 gcgctgcagc gcggtggcgg tctcagagct tggcatggat cgctccgcct acgcggcctt
     4921 ggtcttgaag tctcgcccga cccagccctt ggccagtagc aggaggacgg gtgtggtgca
     4981 ggcgatgcag gcgaaaatca gccacatggt gcgcgtgtcg cgcacgccat cggccgggca
     5041 gaacttctcg agcatgatgc cggaatacag cagcggcacg agcatcttgg tcaggaacca
     5101 cgggagctgc gccacgccca tgtactgccc ggtgcggcct tcgggggcga tttcggcggc
     5161 gtactgaagg aaacgcggct gccacatggc ctcgccgatg gtcatgatca gaatgtagcc
     5221 ggccaaagtt aaggggtagg gaccaatcgc caggaggaag gccggggcgg ccatgatcaa
     5281 ggtgccgatg accatcatgt tgtagacttt ggctttttgc gtggccgcgg cgatgatcgg
     5341 cacgagaatg aaaatcagga ttgggttggc gttcgaggcg atctcgaaat aacgcccaat
     5401 ccagccttca taagcgcgcg agatgtactg gggcagaatg agccagttgt aggtgaatag
     5461 cgtctgaacg ggcatcaagg caaagatgaa gaagaagaac ttggagttgg ccagcggatg
     5521 ttgcgccacc gcgctgcgca ctttgggtat gatcgccgcc gcgatcgccg cgacgaacac
     5581 cacagccatt atttggtagc gccacgcttc cggagcgcgg aagtagatgg ccaccagaat
     5641 tcccaacaac acgagccaca tgtgccaggg aatgcgggca tccacgacga gctcttccgg
     5701 cttgacgtcg ggctgagcct tggttgggcc ggccgcggcg gatgccttct gttcggctgc
     5761 gttgagggcc gccgtttccg cgagggcggc ctcacgcgcc tgtttaaagg tgcgtgcgga
     5821 caagatcaac acagtggaga gcagcgcgat cagcgtgaag ccggtgtaca cccagtagac
     5881 gcccggaatg cccatcccca ggtaatcatc gtcgcgcagc aggaatgcga acgtcggcag
     5941 ccagccgccc aaattcatca gtgcgtagag catcgcaaac gccatgccgg ccgtcttggg
     6001 cgtagtaatt tgccggaccc cggcgtaagc cgccggctgg tacatgccgt agccgatcac
     6061 gaccagcaga attccgccca tggttaccag gtggagctgc gaccacaggc cgctgggctg
     6121 caaattcaga acggtcggcg cggcagagat caacgcgcgg cccaccaaca ttaatgagag
     6181 tgcggctatc aaagcccgcc ggacgcccca tttgtccgcg acgaagccca ggaagaacat
     6241 ggaaatggta atgcccgccg tgagcaccat aaccatgttg tgggagtgca cgtcggcgtg
     6301 tgccattcca gcgaagatga agtcggagaa gtgcttcgcc agatagctca gcactccgaa
     6361 gtagaccata ccctcaagca cataggccag atttattccc cagagcgccc gcggcgcatg
     6421 cgccaggtct atgaacggct ggacgatctc ctgcagggga ctctttttga ccacggtttc
     6481 ctcagtgact tgcgtagcca taggggaagc ttcctttttt cggcgcgcca accggggccc
     6541 aggctcggcg ggcgaccggc gcggcggttt acaaagttcg ggttaggcta gccgcccgcg
     6601 ccgcaccgga caaggcgcct ggccggcggg aacagaacgt ccggatattt cggaatcgcc
     6661 gtcgttgatc cgccgaacgc actctaccga gtggttggcc gcgttccctg ggggaggggg
     6721 gcgcgcgcca ggactaacaa ctaactacta acaacttaac aactcttcgc cccccgcccc
     6781 gggccggctg cgcagcatca gaggcgtggc aggattaaag caggactcgg gcgcggacgc
     6841 ccgcgtttcg ccaccgcccg cccccgcccg tcatcgcctg cttgaccggc cggcggatcg
     6901 tttgccctcg gcccgcttgc cccgccggcc attcccgttt ctattgccct tggagcgcgc
     6961 cgccttcgag ccgccgccgc gcatgctgcg ccgaattaag tcaatcaggt ccggctcagg
     7021 gccggcggcc tccgccgtct cgggagccga aatgacatcc cgccgctccc gccgcttgcg
     7081 ctcgataagc ttgcgaaagc cttcggcgta ttcgtcctgg agttcggccg ggtcgaagcg
     7141 cttgccggcc agcttctgga tctcgcgctc aaagcgcgtc cgctcggccg gactcgcctc
     7201 aacgttctgc ggaagcccca cgtcctccgg actcctgatc tcatccgcaa accgcagcgt
     7261 ctgggcccgc aagatgccgc cttcggagaa aatcgcgacc aggtattcct tgccgcgcat
     7321 gacgaacgtc gccacgcccg cctgtcccag ccgttccatc gtgctcgcca gcagccggta
     7381 agccttgttc gaatccgacg ccggcgccag gaagtaggcc cgctcgaagt acatcggatc
     7441 aatgtccttg gcgtccacga atagccgcaa gtcgatgtcg cgcgacttct tcggctccag
     7501 cgcttcaagc tcctcatccc gcaccaccac gtattcgccg ctctccagcg cgtagccgcg
     7561 gatgatgtgc tccgggtgaa tttcgcgatt ctcgcgcggg cagtagaacc gccgcttcaa
     7621 cggcgtcccg tcctgatcca gcatgcgcag cgagaccccg cccgcccggt ttgcgggata
     7681 catgttcacc ggcacgctca ccagcccgaa gctgatcgtc cccgaccata gcggccgcac
     7741 cggcgcctcg cctggcggcg gctcttcaat atccaccggc cgcggctgct ccggctcggg
     7801 ccggcggggc accctgctta ccatagcgcg gcctccttag cgttcgcgtg actctcaaac
     7861 atgggccgcc tccttacggg ccgccttgac gctcgcggcc agcaggtccg ccaacggttt
     7921 ggccggcgct ttcttcggtt taggcttctc gagccgaatc cgctcgccgc gggccttgct
     7981 ctccacgagc ttcaacacgc gctgccggta ctcgtcgcgg tactgctccg ggtcgaactt
     8041 gccccggaag accgagacaa gctgctccgc catcgcctgc tctttcttgt ccagcgcccg
     8101 gccttgtggc ggttcaagct ccgaaaccgg caccacctcg tccgcgtgcc gtagcgtgtt
     8161 cagcacgagg tagcctttct cagcccgcag cgcccccacg tagctgcgct tgcgcatgac
     8221 ccagcgcgcc agcccttcgc gattggtctt cgccagcgcc tcagccagcg cgaagtactg
     8281 ccccgcgttc ccatccggcc cgaggtaata cggccggtcg taccactggt tattgatcgc
     8341 gccggccggc aggaagtgtg tgatctcgat gtcgcgcgac gattcgggca ccaactccgc
     8401 cagctcttcg tcctccagca gcacgaagag ccccggctcg gcctcaaaac ccttgcgaat
     8461 ctcatcgctg cccaccgtgc gcccggccac cggattcacc atccgctgct ccagccgcac
     8521 ctggtcctgg tcatgcagca ggtggaagtg gatatcgcgg tcctgcacgg ccgaatagac
     8581 cttgacgggc acggccgccg aaccgaactt cagcacacct ttccaaatcg cgcgcgccgc
     8641 cacggatact tctcccggca caggccgcac tgcgccgttt ctaatgactg actgctaacg
     8701 gctgactgca cctgacggcg ctgcacctaa ccgtgctgcg cctaactgcg cgctaactgc
     8761 gccgttttct aaaaactgcg cccaactgcg ccgtttctaa tatgggtaac cccgcgaggc
     8821 ggcctaaaat gctggagaac ccccgaaacg ccgctgaatt gcacgttaac tcgacccacg
     8881 cccctcaggc gaattccgcg cgtagttgtg ggaaggtgag ggaatgtgtg aaagtggccg
     8941 gggccaggac taacgactaa cgactctggc agggggatag gaccgatagg acaaggattc
     9001 gaggcgcatc cgctggcggc ccgagcgctc tttatcgtcc gccgggttcg aaacggagac
     9061 gtccccgttt cggaacccaa ctggacgcgg ccccgtttca cacgccgcac tagcccaaat
     9121 actccccaac tgccctattc caggtgcagg cggggcgcct gttccgcctg gtggtatcaa
     9181 agagaccggc cagcccgtaa caagagctgg ccggtttgga caacccagtg cggggcatta
     9241 tcacgcagga ctactgcttt ctgaagacgc tgcctcgtcc acttgccgca gcaaacgctc
     9301 taatctgctc ggcggtggcc tggaagcgcg gcttccacag gagttgaatc caaatgcccg
     9361 cctgcatagc ccggattttc ccatcgcggt tgtcccaggc gcacacagag actttctcgc
     9421 cgttgcgccc tatggcgtca catagggcaa accgggacag aacgagtccg ccttgcgggc
     9481 ccaacgcgcg ttccgatcga ccgaataccc ggccgccgcc agtacctgcg gccaactcga
     9541 tgccgtttcc cgactgacag gtatgagcga tgacttgccg cgccaaaaca taccgactcc
     9601 gtacagcctg cacagaaaat cccagaacgc gcctgtgagg cattcacgcc agcattatag
     9661 aggaatacgt aggtcgtgaa tacgaggccg cccgctccca ccaatctgat gacaggattc
     9721 attcgtaaac ctcacggatt tcctgcattc tcagctccca gggccacttc gcgcccttac
     9781 tgccacaaga ggcgtcatca tccggcatgc tcgtgctcgg agtgctgtcg tacccatgta
     9841 gcaaagtcgc attcgatttg ctccatcggg ctacggaacg ccttctccag gtctgtgatg
     9901 aacatgcccg gttccatcgt cttggccagc aagaaacggc gaaaagcggc ccagggctcg
     9961 gcattggtgg ccgagatctg ccacaagtag gcgacaaaca ggaggcacgc ctcgtggtac
    10021 gcgcctgaac cgaagtaatc ttcggggggc gtctcgcact tgaacaactc aggtagccgg
    10081 agggctttgc cctcgcgcgc cgtgcgacag tagtgtgcga ccgcgtccgc gtcgagtgca
    10141 caacgaccaa acgtcaggca gttagccaca tgccgcgcgt agccgctcac gccccagcca
    10201 cattcccacg tgattccgaa cgtctcagcc agacgggcca agcacatacc gtagctcagg
    10261 tcccagcgta gttgcagctc ggcgtgggcc tccgggttcc gaggtgctag cgcaagggcc
    10321 ttgcgcacgc aacgctccgt ctgcggcgcg cggaggtata ggtccgccag caatagccaa
    10381 ccctgggcct cagtccgcgc attccgcgag cggggcagaa gccggcgaat gcgcctcctc
    10441 aggccagcgc gcaggcgcgc cgccgtgctg aagtcccgct tcaggtacgt ggtgaaatac
    10501 tcctcaagct cgccccatgt tattctctca acaggcacgg gttgagtccc agatgcctgg
    10561 gtaatgaaag cgcttcggtt tatcaatcca gcgctccttc gaagagtttt cgtccgccag
    10621 gtacgcggtg aatccacggc ccgtttggat gtccgtgccg acggtcctcg gacggtggag
    10681 gtgtcagtcg tccctacggg actggggcga atgcaggctg ttcgcggttc ccaggatttc
    10741 atcctgggct acggtcatgc cgtgcctacg gcacttgtac gctggcattg gtcgtgcggc
    10801 cgcggcatca ttatctggac ggcgtccgca catcctgggc actcaaacgc tggcaggcgc
    10861 cgttttgtga aacggcgtgt gaacggtggg tgaaacggcg acgcagagct agtttcttac
    10921 ggaaggagtt ggctggtgag ccgctggtca tggcagccca agttgatcga tcgggcgtgc
    10981 gtacggccct gacgtgcgcg aaaatggaag tccgccgagg cgcgcgagac ggttcacgaa
    11041 tcggcgctac cctcattggg cggtgacggt ttcggaaaca accgtcaccg gcaatcccgg
    11101 tacgcggcga aagcaggggt cgttggtgat gaagagcgct gccgcggcgt cgagggcggt
    11161 tgcggcgtgg atggcgtcgg gcgttttcag cccgagctcc gcacgaaggc gcgcagcgcg
    11221 ctcgaggatt cgcaactcca aaggcgcaag atggaattcg gtcgagtcga acaagaatgc
    11281 tcgaaacgcc gcttccaggc gggcgttcgc gtcgcgcagc ggcttgacca gcgtttccaa
    11341 cactaccagt tcgctgctga ttaccgagaa ctgaccggac gcggctgcct gccagagagg
    11401 tgcaagcgcg gtgtcgtagg gcgcgacctt ctcaaccgcg tagatgatgc agttggcatc
    11461 aaggtagacg acgccggaag gcggcagatt cagcgctccc atgactggcg ctcttggcgg
    11521 atgtaggcgt ccacgtctgc gggcgttttg aagagcagcc cgccggggca ttcagccagg
    11581 atatcgagca ccgaccgccg cccgttcggc tgctcatgca cgaggcgaat ggtcacctcg
    11641 actggttggc cgggcggcag atcgggagcg cgcacttcga tcttcccgcc cgcctgcacc
    11701 gtcgtattga tctttcgcgc agcgcccaag atggtgtccc tttcaaccag aattgtacgg
    11761 cccgacagtg ccgcgggaaa gtgcgctggg gtcgaagcaa atacgcgcga agtctgacgt
    11821 taacgggctc ggtagccagc cttgcggcac ttgcagggcg ggtttcaatt gccagtcatc
    11881 tatttgtcag aggcggttgc ggccagctat gccagcgctg cacgctgggc tacgctcaga
    11941 ggtggcgaaa gtctgggcgg ggtactgcgc ggatgcggag gcctcttggt ggcatttcgg
    12001 tacgccccgg ctctgctcgc acaaaaggcg agttgcgacc gttggcatca ttccggggcg
    12061 gcaggccccg gctctgctcg caagggcggc cgcgcgggtt ggctgggcga cggaaagccg
    12121 tcaggtaaag atggtcagcc tctcggccgg ggccaagaat gacggtcagg gttcaccccg
    12181 ttgggaaagc cccctagcgg tcaggtatac aaaaggctgt tcatcggtca ggtccagaaa
    12241 taatttgcgc tggcgctcaa aaaaagtgct tttcgcttgc gtaattcctt acgtgtgtta
    12301 cactatgtgt gtcagtggtg aaaagtgggt cgcagtgggc cgaaacgatg ttcctggtcg
    12361 ggacttatga gctggtcatc gacgccaaga accggctctc cgttccgttc aacatccggc
    12421 ggaagctcga tgagcattcg ttttatgcct tgcccggcgg ccgaccacgc actctggcgc
    12481 tttacccgga gtcttatttc gagaagctcc ggcccgtgcc gccagtagaa agtctgtccg
    12541 acacgaccca agcgtggcgt cagttcgagt acagccagtg tgctttgctc gaaactgaca
    12601 accaaggtcg gattctgctt ccggacaggc tgcttaagcg ggccggtatt gaaaaagagg
    12661 tcacactgat cggcgttcag gatcacttgg agctttggaa ccggcgcgac ttcgaagagt
    12721 tcgaaaacag ccagtgggag cacttggctg agaatcgcgc ccgggcgttg caggagttgc
    12781 gagccttcgc acctcagccg tccggcctcc aggcctgaag cgcactcagg acgccagacc
    12841 gcggcctgag cgagatccgg tcagtgacca gatgatacag gttggttggg tttgaacgag
    12901 ggcggatagc agcggcgtct gttggcgcgg tcgtcgctgc atccctcccc acgatgcgga
    12961 gtaacagtca gcgccttttc gccgagggac gctggcgggg aacacggatg ttcctgaact
    13021 ccgaacggcg gttggttccg cctggtgcgg gtcgcaggcc gatcagggaa ggttggccgc
    13081 gaccaagcat ttcatcctga gtacgaacga cgtgtgttgt acccgtttgg ggcgggtacg
    13141 tccatgcttc ggcgaagttt tttttgaccg tcagccgcgt ggcaacgggg caacagatcg
    13201 tggctgatct gcggcgcgaa catcagccgg tattggtcga gcctctgctc gatctattga
    13261 aactggaacc gggccaagtc gttctggacg gcacggttgg gctgggcggg catgccgccg
    13321 ttttcgcccc gcgaatcatg cccggcgggc gctacatcgg cctggacttg gacgaggagc
    13381 tgttggtcga ggcgcggcag cggctgtccg ggctggctgg acacggcctc gacttgttca
    13441 aagcaaatta tgcggagtac cccgaggtcc tggcggccct gggcctgcag cgagtggatg
    13501 tgctatttct ggacctcggg gtcaactctg cccaactggc tgatccggcg cgcggcttct
    13561 cgttcgaaag cgaagggccg ctggatatgc gctatgaccg tacgcaagcc gacaaggcgc
    13621 tcgacttggt gaatgcggcc agcgaggatc agttggccga cctgttttac gaactcgggc
    13681 aggagccact cagccgcaag atcgcgcggc ggatctgcca ggtgcggcat tccggccgga
    13741 tcacgaccac gcgcgtgctg gccagcgcag tcgagtcggc ctatgccgcg gcgggaaaag
    13801 ggccgagccg gcgcagcccg gcgacgcgcg tgttccaggc gctgcggatc gcagtgaacc
    13861 acgagcttga aaacctgcaa aggtttctcg atctggcgcc aacgcacctg gccccgggcg
    13921 gaaagctggc ggtcatcagc ttccacagcc tggaggacgg gatcgtgaag cgctggctgc
    13981 gaggccagga cgatatggag gaaatcacgc gccgcccggt cgtggccgac cccgccgagc
    14041 ggcacagcaa tccgcgcagc cgcagcgcga aactgcgggt ggcgcgccgc atggaagttt
    14101 gaaaagctgt aggagcgcgg caatggcgtt gaattttaag gcggctttgg gcatctgcct
    14161 gttcttcatt gctgggttgt gctatgtcgt gaacaacgtt gcattgccta gccaaccgtc
    14221 gatctcgcca gcagcgaccg tcggcggcgc cgccttgcca ggtgaacgct caaacttcgc
    14281 ggccggccgg ctggagcgaa agaaccccgt cgagtcgcag cgcttggccg ggcgccgggc
    14341 ccgggccgta gccgcggccg agggagcgcc acccgaaacc cgcacggtcg ttcttccgcc
    14401 aatcgtgcgg caggagacgc cgagcttccc ggcggcgctg gcggcccagc cttctgtgcc
    14461 aacccccgag gcgcacgcag gcgaagcggt cgtcgcgttg gcacccgccg gcgcactgct
    14521 gcgtaccaga attgttgaag aaacggatca gtcggcgtgg gaggagacgc cggcccaagc
    14581 tcgcccggtg actctgacgg ccattgaggc gccggaaccc gccgccccgt caaacccgcc
    14641 ggcggctgag ccggttgccg tggcagcggc ggccggcacg tacaaggtcc gcaagggaga
    14701 ttcgctcacc cgaatcgcac agcgggtgtt cggctcgtcc gaacagcgtc atcagcagat
    14761 gttgatcgac gcgaatccca agatcgccaa gcgagtaaac aagctgtacg tgggcgaggt
    14821 gctgaccatt ccgagcattc cgggcgttgc cgccgcatcg gaaagggctt cggggcagcc
    14881 gtccgggacg gtcgccgtca agcaagctga gtcggacggc aagaaagaat ccaagccggc
    14941 gacggtcgca gcggtcgcgg cttcgacgac gaagaagaag agcgccagcg ccgataacag
    15001 tggcaagggc acgaagcgaa cgacggtagc ggcggcgcaa cggccgtcgc gctccggcaa
    15061 gaagggccgg aaagcgagtt cgggcggcga gacagcgaca gagcgttggt atacgatacg
    15121 cggcagcgat tcgctggcga gcatcgctcg cgatgttttg aaagattccc ggcggtggcg
    15181 cgaactcgca gacgtaaacg gattacgcga tccgaacaag atcattcccg gcatgcgcct
    15241 caagcttccg cctgctgggc cgcggcaggg ctgagcggcg cgcccggggc tgggccggcc
    15301 acgggccaaa tgaagccatg acgctaacgc ggatcattgt agttctggtg ggggcgttgg
    15361 cggtcatgat gacggtcata attctgcgtt cggagagcac gcgccagcag tacgaaatct
    15421 cggtgctgga agggcgcgag gacgtgctgc ggcaggagtt caaccgggaa cagctcgcac
    15481 tgcaacgttt gcggaacccg gcggatttgc tggagcgcgt gcagaagatt cggttagcgg
    15541 cgccgcagcc gaccgggccg ccgccggcag cgcagcgaag gcggtccacg gggccgtagt
    15601 ggaggagaac ggagcaggga tgcgctcggc tgcgtggttg tttctattgg tcgcggcgtt
    15661 gctggcggcg ggcgcggggc ggttggccta cattcagtac gcagaagggg acgcgctgcg
    15721 ggagcgggcg gagcggcagc ataccgcttc agttgtcatt cccgcgcagc gtggcgacat
    15781 cctggacgcc caggggcgcc tcctggccgg cagcgaatcc tggccctctg tgtacgctga
    15841 tccctcgctg ctgcaggacc cgcgtgaccg caagttcgcg gcctactcaa tcgctccggt
    15901 tctcggcttg gcggctgagg acgttgacgc cttgctgcgt gaccgcgccg agagccgctt
    15961 cgtatggctc aagcgtcacg taagcccgga ggagctggcg gcgttccata agctgcgcaa
    16021 cgcgcgacgc ctgtatgcgc tggacgtgca atacgagccg gcccgcatct acccgcatgg
    16081 ccggctcgcg tctcacgtgc tgggcttcgt cggggcgccg gaagaaggcg ttcggcacgg
    16141 cctggcgggc atcgagttgt cacaagacaa atatcttatt ggtacgcccg gtcaccgcac
    16201 tcgcacggtc gatgcgcgcc gccgcccctt gtgttcgcac gtagacggtt tcgttccgcc
    16261 gcaagacggc gccacggtca tccttacgat tgatgcctat ctccagcagc agactcagac
    16321 gcacctgaag aacgcggttg agacgttcaa ggcggcctgg ggaacggcgg tgctcatgga
    16381 cccgcagacg ggcgaagttc tggctatggc caccttcccg gatttcgacc cgtccgaccc
    16441 gctgcccgat gacaccaagg ctggcgaacc tgacgaacgg ctgcggaatc gcgccgtttc
    16501 ggacgcgtac gagccgggca gcattttcaa accgttcgtc gcgtcctgtg cgttggaaga
    16561 gaacatcacg cgcctggacg aagtattcgc gatcaacggt cccacgcaca acttcggccg
    16621 ccgaatcatt cacgacacac atgcctatgg ttcgctgccg ctgcacgagg tgatcagccg
    16681 ctccagtaac attggcatgg ccatgctggg gacgcgttgc gagaacgcgc gcctgtaccg
    16741 ttttttgcgg ctgtttgggt ttggcgaccg gacgggcatc gagttgccgg gcgagcacac
    16801 gggcatggtg ttggattttt cgcgctggac cagctactcg acccaatcgg tgccgatcgg
    16861 tcaggaggtt tcggtaaccg cgattcaagt ggccaccgcc ttcagcgtct tcgccaacgg
    16921 cgggattttg tttcgcccgc ggattgtgcg cggcgtggtg ggaccggatg gcgagccggt
    16981 gtgggacaac tcgcggccca ttcccatacg gcgtgtgctc tcgcgcgaag tggccgagcg
    17041 ctttcgcgag gaagccctga tcgaagtggt aatgtcgaag atcggcaccg ggaagcgcgc
    17101 tcgaattccc aactatcggg tctttggcaa gaccgggacg gcgcagattg cgcatgccgg
    17161 cggcggcggt tacatgggcg gcaagtacac cggttccttt gtcggcggcg ctcccggaga
    17221 caagccgcgc gtcgtcgcga tcgtctcgct gagcgcgccc agcagcgggc agtattatgg
    17281 gggcacggta tcggctccgg ccgtgggcga aatcctggcg gacgcgctgg cgtacatgca
    17341 ggtgccgccc gaagtgactt ccgcggacga aaacgggata atcccggggc gtaaggcggg
    17401 ctcgggcgtg caggacgccg gcggttggta agaaatcagt ggcggagaga gtttcagtgc
    17461 gcaagctcgc cgaacttcta aatgggattg tggatcaagg ggcctacgcg ccgcacggcg
    17521 accggatcgt aaccgggctt tttgacgact cgcggctggt gcagcccaat agcgtttttg
    17581 tggccgttag cggcacggcg acggacgggc ggcgcttcgt gccggacgca gtgcaacgcg
    17641 gcgcgggcgt ggtgataggg gagggcctgc cgtcgctgaa cggggcggta accgtgaacg
    17701 tgcccgacgt tcgtgcggcg ctggcgctgc tggcggttcg ctggtacggc ttggacgacg
    17761 gcccggcgcg tgact
//
LOCUS       DAOSYS010000047        17614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2661_length_17614_cov_5.475426, whole genome shotgun sequence.
ACCESSION   DAOSYS010000047 DAOSYS010000000
VERSION     DAOSYS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17614
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_17614_cov_5.475426"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="rplB"
                     /locus_tag="PLU99_04540"
     CDS             complement(<1..778)
                     /gene="rplB"
                     /locus_tag="PLU99_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOO16358.1"
                     /translation="MGVKTYKRTSAGRRNSSVNTYEEVTHRLQNRPAKRLLEPLQKRG
                     GRNHHGVITARHRGGGHKRMYRIIDFRRNLDNVPAKVLEIQYDPNRSCHIGLVEYQTG
                     EKRYILAPLGLKAGDTIESGVKVEPQVGNCMPLASIPIGMDIHNIEMNPGQGGKLVRT
                     AGGAARLMAREGNWAVLQLPSGEMRQVRVECRATIGQLGNLDHQNVRLGKAGRSRYMG
                     WRPHVRGKAMNPVAHPLGGGEGRSNGGRHPCSPWGKLAKGG"
     gene            complement(804..1097)
                     /gene="rplW"
                     /locus_tag="PLU99_04545"
     CDS             complement(804..1097)
                     /gene="rplW"
                     /locus_tag="PLU99_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOO16359.1"
                     /translation="MDIYHTIIRPLVTEKSSYQSRQSSAGRGGTYSFEVHPEANKLQI
                     KNAVEKIYGVRVLDVRTSNRPGKLRRFRYKTGRTRQIKKAVVTLDQDSHIDLF"
     gene            complement(1100..1744)
                     /gene="rplD"
                     /locus_tag="PLU99_04550"
     CDS             complement(1100..1744)
                     /gene="rplD"
                     /locus_tag="PLU99_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOO16360.1"
                     /translation="MLSVPVYDETGAKIGAEQIDEGLLGNEVNASLLKQVVVAYHANR
                     RQGHAVTKSRSDVVGSTKKLYRQKGTGRARMGNARTPQRRGGGMAFAKRVRDFRQALP
                     KKMRRLARNQAVLAKINSNEALIVDGLRFDVPKTSRFATFLKKVDADNGCLFAMAEPN
                     ETLYKSGRNIPRTEIRPVAELNAYDILARRKLIFTRDAFASFRASVGGAQKASE"
     gene            complement(1758..2414)
                     /gene="rplC"
                     /locus_tag="PLU99_04555"
     CDS             complement(1758..2414)
                     /gene="rplC"
                     /locus_tag="PLU99_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOO16361.1"
                     /translation="MVPAILGKKIGMTQVFDPSGERVPVTIVQAGPCVVLQTKQADGK
                     DGYNSVQLGFDDVKPHRSTMPEIGHARKAQTAPKRFVREIRLAEPPDKQIGETVTVEL
                     FEQSGVKYVDVIGTTKGKGFQGGMRRWGFGGQPASHGTERKHRSPGSIGGRGATRGHS
                     GAVKKGKRMAGHTGNERRTIRNQPLVSIDAEHNLLVIRGAIPGAKGSYVIVRKAKTRE
                     "
     gene            complement(2532..2840)
                     /gene="rpsJ"
                     /locus_tag="PLU99_04560"
     CDS             complement(2532..2840)
                     /gene="rpsJ"
                     /locus_tag="PLU99_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOO16362.1"
                     /translation="MAGERIRIRMEAYDHRALDSSAQEIVDQAKRTAARVRGPVPLPT
                     RIERYTVLRSPHIDKKSREQFEMRTHKRVIDIFEPTARTVEALNRLVVPAGVFVKIKA
                     "
     gene            complement(3251..4126)
                     /locus_tag="PLU99_04565"
     CDS             complement(3251..4126)
                     /locus_tag="PLU99_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOO16363.1"
                     /translation="MRPIRLTRARAFTLLLLAAAVLALLPPTWTRWLRSPFQILALPQ
                     WAVLRPAQRACEGAAEARQSERVARLERENEQLRLALGQERLWREDLERRYDAVCGLR
                     GQMWSDNVDLVIAPVLGFDVSPWRETALIGRGADSNLAVGQWVAAGEGFEGWESGAEF
                     LLQRCIIGRIVDVETHVSRVELTTDPKFREQVVPVKVLADGTMQPEPRPYVITGAGRR
                     GMMISQADADLSKLGFTYLALPARPGLPATLSVGKIKSSKQSAQSALHYDIEAEPLVN
                     VARLRYVAVIAASAQ"
     gene            complement(4272..5324)
                     /locus_tag="PLU99_04570"
     CDS             complement(4272..5324)
                     /locus_tag="PLU99_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOO16364.1"
                     /translation="MFLRRVMGLFSIDMGIDLGTCNTLVCVRGEGIVLNEPSVVAVRK
                     GTNQVLDNGNAVGLVAKEMLGKTPGSISAIRPLKDGVIADFDITEAMLGYFIRKVHGN
                     RGLAHPRVVIAIPSGITAVEKRAVYNSAERAGARRVYLVEEPMAAGLGAGLPIHEPTA
                     SMIVDIGGGTTEVAVMSLSDIAECHSLRVAGDEADAAIINYMKRNYNLMIGLQTAERI
                     KIQIGSVAPLSEEMKMEVKGRDMISGLPRRAVITSEEVREALKEPVGQIIEAVLRTLE
                     KLEPELAADLVDNGIHLAGGGSLLRGLDQVLAKASGLDVRVADDPLTCVARGTSEFLD
                     NLDTWGGTFESDEDEY"
     gene            complement(5428..5598)
                     /locus_tag="PLU99_04575"
     CDS             complement(5428..5598)
                     /locus_tag="PLU99_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16365.1"
                     /translation="MTTAGSGPVRGTRVKADSDIYTVLMVISFLFTLTATIYIGYRVF
                     YLFDTVLPPPGS"
     gene            complement(5605..6459)
                     /locus_tag="PLU99_04580"
     CDS             complement(5605..6459)
                     /locus_tag="PLU99_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16366.1"
                     /translation="MSTMTKVFVVLTAVLSIALSCLFIAAAAQTANWKQLAQDYQDRQ
                     TAEFMHRMNLQAIMEATLSMRDQQLQAKARDLAAAQDLATRQADELKQLQASLAREMN
                     DRVAAQAGQAKLQEILGMQNAEVAALQKQNQMLLAQNIDFQTRNTRLNSRVLELTSNN
                     TILTDQVRNLQEKLYAAEQQAVRTAQGGRPAERATGVVAVSPSLPSPIMGQVVSVDGN
                     YASINVGQASGVTQGMTFMVHRGSNFLADIVVDRVWPDQAGGKLKSIQQEIRPGDSVS
                     YGLDNIGS"
     gene            complement(6733..7227)
                     /gene="purE"
                     /locus_tag="PLU99_04585"
     CDS             complement(6733..7227)
                     /gene="purE"
                     /locus_tag="PLU99_04585"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOO16367.1"
                     /translation="MPGSAEVALLMGSKTDWDIMKAAAETLREFGLTYEARVLSAHRT
                     PDETAEYVKKAEAGGTQVFIAAAGMAAHLAGAVAAQSVRPVLGVPLAASELHGLDALL
                     ATVQMPPGIPVATLAVGKAGAVNAALLAVQIISLGRPELQSALRERRRANAQKILNER
                     LELT"
     gene            7392..8447
                     /locus_tag="PLU99_04590"
     CDS             7392..8447
                     /locus_tag="PLU99_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16368.1"
                     /translation="MRLPVERPEISVVVPVYQEEASLPTVIPRFLESLKAQSRSFELI
                     AVNDGSRDRSGEILDRFAAREPRLRVIHFARNYGQTAALMAGFWAARAPVIVPLDGDG
                     QNEPDEIGRLVDELERGYDVVSGWRKDRKDSWLRTRVSRAANWLIGHITGVRLHDYGC
                     TLKAYRAAMIRDARLYGEMHRFIPVFTSMHGARITELVVRHHPRMAGETKYGYDRVLK
                     VLFDLVLVRMLQRYRTKPMHFFGKITKWSWLAALLCLVFAGVQAALSPTPWQAFWGKA
                     PVVGVMFFLLGGFSIMLGLVAELAMRAAYEFNAGRYWDEARRVNFESSSEDWGVPRSM
                     SADLRSAAVEEVAEAGR"
     gene            8582..10177
                     /locus_tag="PLU99_04595"
     CDS             8582..10177
                     /locus_tag="PLU99_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16369.1"
                     /translation="MPAARGTDVSYARAAYLPVVAAICLFTLLVLRTAWISDDACITF
                     RTIENFLGGDGLRWNVDERVQVYTHPLWLFLLTACRVVTGELSLTTITVSTILSVATV
                     LILAGRLAVAALPALVGVMALALSKAFTDFSTSGLENALMHLLLAGFAVVYFAGEPTR
                     RTVFWLSLLTALLVLTRPDALLLVAPALALTLWRVRAFLPSVLGFIPLIAWELFSLIY
                     YGFLLPNTAYAKLNTGLPQHVLAERGMYYIVQTLQHDSLTLVVLAVGLLTPFLMNARK
                     HMPLAVGACLYIAYVVSVGGDFMLGRFLTAPLLIAVILLARQHLATPTALILLALVMI
                     IGLVPKRAPIYADENYGSRYDGICLVQDERACYYPHTGLLTAFHRRQFLDHICVEQGL
                     AARRADKPVVRSSIGMFGLHAGRETFIVDELGLGDALLARLTPIPEEDWRIGHLHRAI
                     PAGYLETLATGHNQIADPELAAYYDRIALATRADLFDPRRWKAIAELNLGLVRPPRKY
                     VLAQAPTDQGARVRGSSRETSTP"
     gene            complement(10206..11570)
                     /locus_tag="PLU99_04600"
     CDS             complement(10206..11570)
                     /locus_tag="PLU99_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO16370.1"
                     /translation="MRGRILVVDDEPLKRITLQIELSELGFEVFEAADALAARRIFDS
                     KPIDIVVSDVRLPGANGLDLLAHIKQARPDVGVILMTAYASVDTAVQAIKGGAYDYIT
                     KPFTTQELVPKLERLLAVRSEVPRNEEVEQLSQLVAVSQSMKRLFAQVRAVADTDRTI
                     LLCGESGTGKELFAEAIHDLSRRADKPFVRFSCAALQASVLESELFGHEKGAFTGAIR
                     QKPGRFELAHTGTILLDEVDDIPVEIQVKLLRVVEQQEFERVGGETPVHVDVRLLCAT
                     KCDLLKLVKEGRFREDLYYRLNVISLAIPPLRERPDDIPLLARHFLKKHAALTGDRVV
                     TISPAALDQLLRHNWPGNVRELEHVMERALAFCGGHEIRPEHITAPVVEATEPVAWAA
                     GMEPPEGARPGLTDTLSDIERRMILLALRQCDNNQVRAAQRLGIPRTTLRDKMAKYKI
                     PGGC"
     gene            11788..12420
                     /locus_tag="PLU99_04605"
     CDS             11788..12420
                     /locus_tag="PLU99_04605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOO16371.1"
                     /translation="MVEAAQELITRYGYIGLFGLLALGVVGVPAPDETLVAVSGLLIK
                     KGEMSLVPTFLAAVGGSAVGVTISFVVGRVFGFRLIHRYGHLLHVTEERLGRFQRWYE
                     RVGRWTLVFGYFIPGIRQVTAFASGTSGLSWASFAAFAYSGAVLWVTTFLTAGYVLEA
                     EWEKSSPRMHAVIVGVAGVGFAVVATYGVLRLMRYRRNVTPASNSTAPDE"
     gene            complement(12467..>12909)
                     /locus_tag="PLU99_04610"
     CDS             complement(12467..>12909)
                     /locus_tag="PLU99_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16372.1"
                     /translation="ALHTWPSADANLLLLGGADSLTCRMAHYPRSILASRYDMLQDVV
                     LHGLAERRMAATALAIRLYELDYGRRPERLAELVPDYLPSVPGDPFAADDRPIGYVPH
                     APRPVLYSVNIDGVDEGGRYVLYSAGRVERDAADLPFFLNGDRP"
     assembly_gap    12910..13009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13217..14245)
                     /locus_tag="PLU99_04615"
     CDS             complement(13217..14245)
                     /locus_tag="PLU99_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16373.1"
                     /translation="MVVLVARHNAGDDAGALEACNDMLGIAAALADGPLMSQLVSVGV
                     SALAYDGLERITPTLNIEEKGAGDPVLTGPASRAQVSALIEALLDEAECRQAAVRAMH
                     VERMTQIEDAHAFMNGRWGLFSGSPILGTLAPHLLLLGPAWVLDTAFMAEWTTQMANA
                     MAAANWPAAAAHIPAEVTGRSEIDETAHLLSSILLPSYERAVQLSFRALATRRMAATA
                     LAIRLYEIDYGRRPDRLEELVSDYLPAVPRDPYAADDRPIGYAPNASLPVLYCLNLDG
                     VDDGGRFELRTNGAVDGDAADLVFFLNGDRPRPPLAASASAGQSAATQSAPASQSSSV
                     ETMPDDRE"
     gene            complement(14239..14508)
                     /locus_tag="PLU99_04620"
     CDS             complement(14239..14508)
                     /locus_tag="PLU99_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16374.1"
                     /translation="MSPKSGDATQNEKLIPARFWWLKRLACDAVIMLVVLLAVRLWWG
                     WEAQRRFDAAIAKCRAAGEPVLVDDFLIAPLPDDENAAFFLNGNW"
     gene            complement(14678..15307)
                     /locus_tag="PLU99_04625"
     CDS             complement(14678..15307)
                     /locus_tag="PLU99_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16375.1"
                     /translation="MIASIFLRWRVAGILVLGLAAAFLTACWSPPDTAGQRELQTQRE
                     VIIRQNEQLARQKAEIDELTKQLAAARGFTNDDLQNVFYPTKLVIAPLSGGYDIDGRP
                     GDEGVVLYLQPVDAAGDVLKVAGEIRIQLYDLANPPAENLIGQYVIPVEQARDLWYGK
                     LMTNHYTIKCPWPSGPPAHPDITVHATFIEYLTKRVVSTQGVVKVSLPP"
     gene            complement(15498..16127)
                     /locus_tag="PLU99_04630"
     CDS             complement(15498..16127)
                     /locus_tag="PLU99_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOO16376.1"
                     /translation="MNNQLIPIVIEREGRAERAYDIYSRLLKDRIIFLGDEITPELTN
                     VVIAQMLFLANEDKNADIHLYINSPGGAVYSGMGIYDTMQFVPCPVATYVIGVAASMG
                     AVLAAAGRPGKRFVLPHSRTMIHQPLGQSRGPATDLKIELDEMLRTQEHLYRVLAKHT
                     GKPIEQIAADCDRNNWMDAEQTVAYGLADSILEQMPDKPALRATPPEQS"
     gene            complement(16146..16781)
                     /locus_tag="PLU99_04635"
     CDS             complement(16146..16781)
                     /locus_tag="PLU99_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOO16377.1"
                     /translation="MAYWPPVERPRMQSQMQYQRQMGLNELLLENRIIFLDLPLDPNV
                     TINASTVCSHVIKSMLYLQSIKRDQDIHLYINCPGGDIDDTLAIYDTMQFLNSEIATY
                     CVGAASSGAALILAAGTKGKRYALPHAKVMIHQPWGYMGGQAADMRIQADEILKVKKT
                     ITEILARCTGRSSEQIAKDIERDRYMTAEEAKEYGLIDEILEESKPKKSKK"
     gene            complement(16869..>17614)
                     /locus_tag="PLU99_04640"
     CDS             complement(16869..>17614)
                     /locus_tag="PLU99_04640"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16378.1"
                     /translation="DGVQVPDLDKVLEGAKPGETREATCTIPDDYERADIRGQKGRFT
                     FRIHELKRLVPMPLDDFLQRAGFDKEADAFDHFRMMMERDRDEMIERAKRAQVEAFLL
                     EKTSLELPEKLSARQIDRAVVRRMIELRQRGVPEDEVLARADALRSSAKEDVTRELKL
                     GFILEKAAEKLEIEVTDEEVNTAIAAMARRYNRRFDRVRDELQQQGLLSQLAERIRQD
                     KTVSMLLEKAKVTGVKHESAGAESAAEPT"
BASE COUNT         2935 a         5883 c         5284 g         3412 t
ORIGIN      
        1 gcccgccctt ggccagcttg ccccagggcg aacagggatg ccgcccgccg ttgctgcgcc
       61 cctcgccccc gcccagcggg tgcgccacgg ggttcatggc cttgccacgc acgtgcggcc
      121 gccagcccat gtatcgcgaa cgccccgcct tgcccaggcg aacgttctgg tggtccaggt
      181 tgccgagctg gccgatggtc gcccggcatt cgacgcgcac ctgccgcatt tcgcccgacg
      241 gcagttgcag caccgcccaa ttgccctcgc gggccatcag ccgcgccgcg ccgccggccg
      301 tgcggaccag cttgccgccc tggccggggt tcatctcaat attgtgaatg tccatgccga
      361 tgggaatgga cgccaacggc atgcagttgc ctacctgcgg ttcgaccttc accccgctct
      421 caatggtgtc gcccgctttc agacccagcg gcgccaggat gtagcgcttc tcgccggtct
      481 ggtactcgac caggccaatg tggcaggagc gattcgggtc atactgaatt tccagcacct
      541 tggccggcac gttatccaga ttacggcgga agtcgatgat ccggtacatc cgcttgtgcc
      601 cgccgccgcg atgccgagcg gtgatgaccc cgtgatgatt ccgcccacca cgcttttgga
      661 gcggctcaag cagcctcttg gcgggccggt tctgcagccg gtgcgtcact tcctcgtaag
      721 tattcacgct cgagttgcgc cgcccggcgg atgttcgttt gtatgtcttg acgcccataa
      781 cctacctcgt ttccgggctc cgcctagaac aaatcaatgt ggctgtcttg atccagcgta
      841 acgacggcct tcttgatctg gcgcgtccgg ccggtcttgt aacgaaaccg ccgcaacttc
      901 ccgggccgat tggaagtccg cacgtccaaa acgcgaacgc cgtagatttt ttccacggca
      961 ttcttgattt gcagcttatt ggcctcggga tggacttcga agctatacgt gccgccgcgg
     1021 cccgcgctcg actggcgcga ttggtacgag ctcttctccg taacgagcgg cctgatgatc
     1081 gtatggtaaa tatccatgcc tattcgctcg ctttctgggc cccgcccacg ctagcgcgga
     1141 aactggcgaa ggcatcccgc gtgaagatca gcttccgccg ggccagaatg tcatacgcgt
     1201 tcaattccgc caccgggcga atctcggtcc gcgggatgtt gcggccgctc ttgtaaagcg
     1261 tctcgttggg ctccgccatc gcaaagaggc agccgttgtc cgcgtcgact ttcttcagaa
     1321 acgtggcgaa acgcgaagtc ttgggcacgt cgaagcgcag cccgtccacg atcagcgctt
     1381 cgttgctgtt aatcttggcc agcaccgcct ggttccgggc cagccggcgc attttcttgg
     1441 gcagcgcctg gcggaagtcg cgcacccgct tggcgaaggc catgccgccg ccgcgtcgct
     1501 gcggagttcg ggcgttaccc atccgggccc gcccggtgcc cttctggcga tagagcttct
     1561 tggtagagcc gacgacgtcg gagcggctct tggtcacggc gtgcccctgc cgccggttcg
     1621 catgataggc cacaaccacc tgcttcagca acgacgcgtt gacctcgttg ccaagcagcc
     1681 cctcgtcaat ctgttcggcg ccgatcttcg cgccggtttc gtcatatacg ggtacgctta
     1741 gcatggccct tactccgcta ctcccgggtc ttcgccttcc gcacgatcac atagctgccc
     1801 ttggcgcccg gaatcgcccc gcggatgacc agtagattgt gctccgcgtc gatgctcacc
     1861 agcggctggt tgcggatcgt ccgccgctcg ttgccggtgt ggccggccat ccgcttgcct
     1921 ttcttgaccg ccccgctgtg gccgcgcgtc gccccccggc cgccgatgct gcccggagaa
     1981 cggtgtttgc gctcggtgcc gtgcgaggcc ggctgcccgc cgaaacccca gcgcctcatc
     2041 ccgccctgaa agcccttgcc cttggtggtc ccgatcacat cgacgtattt gacgccgctc
     2101 tgctcgaaca gctccaccgt aaccgtctcg ccgatctgct tgtcgggcgg ctcagccagc
     2161 cggatctcgc gcacaaaccg cttcggcgcc gtctgggcct tgcgggcgtg gccgatctcg
     2221 ggcatggtcg agcggtgcgg ctttacgtcg tcgaagccca gttggacgct gttgtagccg
     2281 tccttgccgt cggcctgctt ggtttgcagg acgacgcacg ggccggcctg gacgatcgtc
     2341 accgggacgc gctcgcccga tggatcgaac acctgcgtca ttccgatttt cttgccaagt
     2401 atcgccggga ccatcccgcg gcactcctgt cagcacgccg cccgccgcgt aggtcgggtc
     2461 tgccgacccg actccccccg gttaccgcgg cgaggccgcg cacaaaaaac gttagtcggc
     2521 agccgcgccg actatgcctt gatcttcacg aaaacacccg cgggcaccac cagccggttc
     2581 agggcctcca cggtcctggc ggttggctcg aagatatcga tgacccgctt atgagtgcgc
     2641 atctcaaatt gttcacgcga tttcttgtca atgtgggggg accgcaggac ggtgtaccgt
     2701 tcaatacgtg ttggcagcgg cacggggccg cgcacgcggg ccgccgtccg cttggcctgg
     2761 tccacgatct cctgggctga agaatccagc gcccggtgat cataggcctc cattcgaata
     2821 cgaatccgct cacctgccat cggcgttcta ccgtccgttc aacatcgtaa cctgtctggg
     2881 ttcccgacca cataagtcga gtcgtgccag aataccgccc gtaacgatta cgtcaagccc
     2941 aaagaacgga aattgcacgc ggcgagcgcc gccggagcat aatcgcggag cggagcgggc
     3001 atatcgcgga gtcgagccgg cggaaaagcg gggcgcaccc gagcagagcc ggggcgtgtc
     3061 gccccggact gatggagccg gacatcgagg gcgatctagg gatcaagtga tcgagaggtc
     3121 tggcggcttt tccgcgtttt gctgacatcc ccccaatttc ggagcccaag gcgcgttcgg
     3181 gcacaccgta caaaccgggg gcggcacgcc ccggctctgt tactttgcgc agcagctcgc
     3241 cgccttgggt ttactgggca ctcgcggcga ttaccgccac gtagcgtagc cgcgcgacgt
     3301 tgaccagcgg ctccgcctcg atgtcgtagt gcagcgccga ttgcgccgac tgtttggaac
     3361 tctttatctt gcctacactt agcgtcgccg gcagtccggg ccgggccgga agcgccagat
     3421 acgtgaaccc cagcttgctc aaatccgcat ctgcttggct gatcatcatg ccccgccgcc
     3481 cagcgccggt aatcacgtaa ggccgcggtt ccggctgcat ggtcccgtcg gccagcactt
     3541 ttaccggcac cacctgttcg cggaatttgg ggtccgtagt cagctccacc cggctgacgt
     3601 gcgtctcaac gtcgacgatg cggccaatga tgcagcgctg cagcaaaaac tccgccccgc
     3661 tttcccatcc ctcaaacccc tcccccgccg ccacccactg gcccacggcg agattgctgt
     3721 ccgcgccgcg gccgatcagc gctgtctcgc gccacggtga gacatcgaac cccaggaccg
     3781 gcgcgatcac caggtccacg ttgtccgacc acatctgccc gcgcagccca cacaccgcgt
     3841 cgtaacgccg ctccaaatcc tcccgccata gccgctcctg ccccagcgcc aggcggagtt
     3901 gctcgttctc tcgctccagc cgcgccacac gctcagattg ccgcgcttcc gccgccccct
     3961 cacaggcacg ctgcgccggc cgcagcacgg cccattgcgg cagggccaat atctggaagg
     4021 ggctgcgcag ccagcgcgtc cacgtgggcg gcagtagggc cagcaccgcg gccgccagca
     4081 gcagcagggt gaatgcgcga gcacgcgtca gccggatggg cctcacttcg gtcccccgtt
     4141 gtgttgccag cgtgttttga ggtcagtccg ttcccccaca gatgttctat cgcaagcgcc
     4201 cccgcgtaac cgcacaaaac gccggcgggg cgcccgtgcc acactcgccg acccgggaac
     4261 agtgggccgc gtcaatactc gtcctcgtcc gattcgaatg tgccgcccca cgtgtccagg
     4321 ttatctagaa actcgctcgt gccgcgcgcc acgcacgtca gtggatcgtc cgccacgcgc
     4381 acgtccagcc ccgaggcctt agccagcacc tggtccagcc cacgcaggag cgacccgccg
     4441 cccgccaggt gaatgccgtt atccaccagg tccgcggcca actccggctc cagcttttcc
     4501 agcgtccgca aaaccgcctc gatgatctgg ccgaccggct ccttgagcgc ctcgcgcact
     4561 tcctcgctgg tgatcaccgc ccgccgcggc agcccgctga tcatgtcgcg gcctttgacc
     4621 tccatcttca tctcttcgct cagcggggcc accgacccga tctgaatctt gatgcgctcg
     4681 gcggtctgca ggccgatcat caggttgtag ttgcgcttca tgtaattgat gatggccgcg
     4741 tccgcctcat cccccgctac ccgcaacgag tggcactcgg cgatgtccga caggctcatc
     4801 accgcgactt ccgtcgtgcc gccgccgatg tccacgatca tggacgccgt cggctcgtgg
     4861 atcggcaggc ccgcccccag cccggccgcc atcggctcct ccacgaggta aacgcggcgc
     4921 gctccggccc gctcggcgga gttgtagacc gcccgcttct cgaccgccgt aatgcccgag
     4981 ggaattgcga tcaccacccg cggatgggcc aggccgcgat tgccgtgcac cttgcggatg
     5041 aaatagccga gcatggcttc ggtgatgtcg aagtcggcga tgacgccgtc cttgagcgga
     5101 cgaatggccg agatgctgcc gggcgtcttg cccagcattt ccttggcgac caggccgacc
     5161 gcgttgccgt tgtcgagcac ttggttcgtg cctttgcgca ccgcgaccac ggagggttcg
     5221 ttcagcacga tgccctcgcc gcgcacgcag accagcgtgt tgcacgtccc caggtcgatc
     5281 cccatgtcga tgctgaacaa gcccatcacg cggcggagaa acatgtcaga ttcctccttg
     5341 aggcatcagc cctttccgcc tgcgtgcctg cgctgcaagc gccctgcaca tgccgcctcc
     5401 ttgcggtgcg ccagcgccgc ggccacctta gctgccgggc ggtggcaaaa cggtgtcgaa
     5461 caggtagaac acgcggtaac caatataaat ggtggcggtc agcgtgaaaa ggaacgaaat
     5521 caccatcaac accgtgtaga tgtcgctgtc cgccttgacg cgggtcccgc gaaccgggcc
     5581 tgagcctgcc gtcgtcatct ctcgctaact cccgatgttg tccagcccgt agctcactga
     5641 gtcgcccggt cggatttcct gctggatgct cttgagcttg ccgccggcct ggtccggcca
     5701 cacgcgatca accacgatgt ctgccaggaa gttcgatccg cggtgaacca tgaaggtcat
     5761 gccctgcgtc acgcccgagg cctgcccgac gttgatgctg gcataattcc cgtccacgct
     5821 gaccacctgg cccatgatcg ggctcggcaa actcgggctg accgccacca caccggtcgc
     5881 acgctcggcc gggcgtccgc cctgcgccgt gcggaccgcc tgctgctcgg cggcgtacag
     5941 cttctcctgc agattgcgaa cctggtcggt cagaatcgta ttgttggacg taagttcgag
     6001 cacccggctg ttaagccgcg tgttgcgcgt ttggaagtcg atgttttgcg cgaggagcat
     6061 ttgattctgc ttctgcaggg cggccacctc cgcgttctgc atccccagaa tctcctggag
     6121 cttggcctgc ccggcctggg cggcgacgcg gtcgttcatc tcgcgggcca ggctggcttg
     6181 aagctgcttg agttcgtcag cctgccgggt cgccagatcc tgagcggcgg ccaagtcgcg
     6241 cgccttggcc tgcaattgct ggtcgcgcat ggatagcgtc gcttccatga tggcctgaag
     6301 gttcatgcgg tgcataaact cggccgtctg ccgatcctgg tagtcctgcg ctagctgctt
     6361 ccaattggcc gtctgcgccg ctgcggcaat gaataggcag gaaagcgcga tcgacaaaac
     6421 tgcggtcaag acgacaaaaa ccttggtcat tgtgctcacg agtgaactcc tggagctccc
     6481 gcgccggcgc atcgggcatc ccacagcggc tcaatcaggc cccgacccgc cggtttggcc
     6541 gggattgtaa ggggcgtgcg aaacattgca agctgcattg ttgaggaccc taagtggtat
     6601 tgcaaccggt agcgcggctc gaagcggcca ggacaccggt tggggacgct cgaaaggcgc
     6661 tttcggggca agaattgcga gccgagctcc gggcagcgcc ggtcgcgcgg ggccgataag
     6721 ccttggctgc gctcatgtga gttccaagcg ctcgttgagg attttctgcg cgtttgcccg
     6781 gcggcgctcg cgcagcgcgc tctgcaactc cgggcgcccg agcgagatga tttgtaccgc
     6841 cagcagcgcc gcattgaccg ccccggcctt gccaacggcc agcgttgcca ccggaatgcc
     6901 cggcggcatc tgaaccgtgg ccagcaaggc atccagccca tgcagctccg atgccgccag
     6961 cggcaccccc agcactggac ggacgctttg agccgccact gccccggcca ggtgggccgc
     7021 catgcccgcg gcggcgataa aaacctgcgt tcctccggcc tccgccttct tgacgtactc
     7081 ggccgtctca tccggggtac gatgggccga cagaacgcgc gcttcgtagg tcaggccgaa
     7141 ctcgcgcaac gtctcggccg ccgccttcat gatgtcccag tcggttttgc tgcccatcag
     7201 aagggcgact tccgcactgc cgggcatagg tgtttcccga ttcgagttcc aagtttcgag
     7261 tttcaacttg gggcgccgtc cgccttgata cgggctgcaa acgccccggc cgcccggtat
     7321 actaaccgcg tgaccgtctt gtagtagaga cgcggattcg aactcgaaac ccgaaatttg
     7381 aaacctgaac cttgagactt cccgtggagc ggccggagat ttcggtagta gtgcccgttt
     7441 atcaggagga agccagcctt cctacggtca tcccgcgctt tttggaatcg ctcaaggcac
     7501 agtcgcggag tttcgagtta atcgcggtta acgatggcag ccgcgatcgg agcggcgaga
     7561 ttctggatcg cttcgcggcc cgcgagccgc gcctgcgggt catccacttt gcccgcaact
     7621 acgggcagac cgcggccctg atggcgggct tttgggccgc ccgggccccg gtgatcgtgc
     7681 cgctggacgg cgacgggcag aatgagccgg acgagatcgg gcgcctggtg gacgaactgg
     7741 agcgcggcta cgacgtggtc agcggctggc gcaaggaccg caaggattcc tggctgcgga
     7801 cgcgcgtttc gcgggcggcc aactggctga tcgggcacat caccggcgtt cggctgcacg
     7861 actatggctg cacgctgaaa gcctatcgag ccgcgatgat tcgcgatgcg cggctgtacg
     7921 gggagatgca ccgcttcatc ccggtgttca ccagcatgca cggggcgaga atcacagaac
     7981 tggtcgtgcg gcatcatccg cgcatggcgg gcgaaaccaa gtacgggtac gaccgcgtgc
     8041 tgaaggtgtt gttcgacctg gttctggtgc gcatgctgca gcgctatcgc actaagccaa
     8101 tgcacttttt cggcaagatc acgaaatggt cgtggctggc ggcgttgctg tgcctggtgt
     8161 ttgcgggcgt gcaggcggcg ttatccccca cgccgtggca ggcgttctgg ggcaaggcgc
     8221 cggtggtggg cgtgatgttc ttcctgctgg gcggattttc gatcatgctc ggcctggtag
     8281 cggaactggc gatgcgcgcc gcttacgaat tcaacgccgg gcggtactgg gatgaagcgc
     8341 ggcgcgtcaa cttcgaatcg agcagcgagg attggggcgt gccacggagc atgtcggccg
     8401 acctccgctc agccgctgtt gaagaagttg cggaagcagg tcggtaaaac ggacactttt
     8461 ccgttcggcg aatgtccgac tcggcgcctc cggccttgcc ggccttgacc cgcgttctac
     8521 gccgagacat cctagaatta tgcaacccgc accccaggag ccgaccgccc gaggcacgta
     8581 tatgccggcc gctcgaggca cggatgtgtc gtacgcccga gccgcgtatc tgcctgtagt
     8641 ggcagccatc tgtttgttca ccctgctcgt gctgcgcacc gcctggattt cggacgacgc
     8701 gtgtatcaca tttcgcacga tcgagaattt tctcgggggg gacggcctac ggtggaacgt
     8761 cgatgagcgc gtgcaagtgt atacgcatcc gttatggctt tttctgctga ccgcctgcag
     8821 agtggtcacg ggcgagctct ccctgactac cattaccgtc tcaacgatcc tgtcggtcgc
     8881 gacggtgctg atcttggccg gacgcctggc ggtggccgca ttgcccgcgc tggtcggtgt
     8941 gatggcgttg gcgctctcga aagccttcac tgatttttca acttctgggc tcgagaacgc
     9001 gctcatgcac ctccttctgg ccggatttgc ggtggtctat ttcgccggag agccaacgcg
     9061 ccgaacagta ttctggttat ccctcctgac cgcgctactc gtactgacgc ggccggatgc
     9121 gctgctgctg gtcgcgccgg cactggcgct gacgctgtgg cgcgtgcggg cgtttctgcc
     9181 gagcgtcttg gggttcattc cactgattgc ctgggagctc ttctcgctga tttattatgg
     9241 ctttttgctg cccaacactg cctatgccaa gctgaatacc ggcttgccgc agcacgtcct
     9301 ggccgaacgc ggaatgtact acatagtcca gacgctacag catgattcgc tcacgctggt
     9361 cgtcctggcg gttggcctct tgacgccgtt tctgatgaac gcccgaaagc acatgccgct
     9421 ggcggtagga gcctgcctgt acatcgcgta cgtggtaagc gtaggcggtg atttcatgct
     9481 cgggcggttt ctgaccgccc ccttgttaat tgcggtcatt ttgctggccc gccagcacct
     9541 ggccacgccg accgccctca tcctgctggc gttggtgatg atcatcggcc tggttccgaa
     9601 gcgtgcgccc atttacgccg acgagaacta cgggtcgcgg tatgacggaa tctgcctcgt
     9661 gcaggatgaa cgcgcctgct attacccgca tactgggttg ttgaccgcct tccacagacg
     9721 acaatttctc gatcacatct gcgtcgagca gggcctggcg gccaggcggg ccgacaagcc
     9781 ggtcgtgcgc agctcgatcg gcatgttcgg gctccatgcc ggccgcgaga cgttcatcgt
     9841 ggacgaattg gggttgggcg acgccttgct tgcgcggctg acgcccattc cagaggagga
     9901 ttggcgaatc ggacacctgc accgggcaat tccggccggc tacctcgaaa cgctggcgac
     9961 cgggcacaac cagatcgccg acccagaact ggcggcgtac tacgaccgca tcgccctggc
    10021 aacgcgcgcg gatttattcg acccccgacg ttggaaggcg atagccgagc taaatctggg
    10081 actggttcgg ccgccccgaa agtacgtgct cgcccaggcg cctacggatc aaggggcccg
    10141 cgtccgcggg tcttctcgcg agacctcgac cccgtaatcg gcgcgatcct gcggcctcgg
    10201 cccgatcagc acccgcccgg aatcttgtat ttggccatct tgtcgcgcag cgtcgtccgc
    10261 ggaatgccca gccgttgggc ggcccgcacc tggttgttat cgcattgccg cagcgccagc
    10321 aggatcatcc gccgctcaat gtccgagagc gtgtccgtca gccccggccg cgcaccctca
    10381 ggcggttcca tgcccgcggc ccacgccacc ggctcggtcg cttcgactac cggcgcggtt
    10441 atgtgttccg gccggatttc atgaccgccg cagaacgcca gggcgcgctc catcacgtgc
    10501 tccagctcgc gcacattgcc cggccagttg tgccgtaaga gctgatcgag cgccgccggc
    10561 gagatcgtca ccacccggtc gcccgtcaac gcggcgtgct ttttcaggaa gtgtcgcgcc
    10621 agcagcggaa tgtcgtccgg ccgctcgcgc agcggcggaa tcgccaggct gatcacgttc
    10681 agccggtagt acaaatcttc ccggaaccgg ccttccttga cgagcttgag cagatcgcac
    10741 ttcgtcgcgc acagcaggcg cacgtcgacg tgcacgggcg tctcgccgcc gacgcgctca
    10801 aactcctgct gctcgacgac gcgcagcagc ttgacctgaa tttccaccgg gatgtcgtcc
    10861 acctcatcca gcagaatcgt ccccgtgtgc gccagctcga accgccctgg cttctgccgg
    10921 atcgccccgg taaaagcccc cttttcgtgc ccgaacaatt cgctttcgag caccgaagcc
    10981 tgcaaggccg cacaactgaa tcgtacgaac ggcttatcag cccggcggct gaggtcgtga
    11041 atagcctcgg cgaaaagctc tttgccggtg ccgctctcgc cgcacaacag aatcgtgcga
    11101 tcagtatcgg ccacggcccg cacctgcgcg aacagccgct tcatgctctg gctgacggca
    11161 accagttgcg agagttgctc gacttcctcg ttccgcggga cttcgctgcg cacggccagc
    11221 aggcgctcca gcttgggaac cagttcctgg gtcgtgaagg gcttggtgat gtagtcgtac
    11281 gcgccgcctt tgatggcctg cacggcggtg tccaccgagg cgtaggcagt catcaggatg
    11341 acgccgacgt ccggccgcgc ctgcttgata tgcgccagca ggtctaggcc gttggcgccc
    11401 ggcaggcgaa catccgatac gacgatgtcg atgggcttgg aatcgaagat gcgccgcgcc
    11461 gccagcgcat cggcggcctc gaagacttcg aagcccagtt cgctgagctc gatctgaagc
    11521 gtgatgcgct tcagcggctc gtcatcaacc accaggatgc gaccgcgcat gccctgtccc
    11581 tcacaattcc aatccgcgct gccggatcaa ttcgtcgccg cgccgcgacg gccagcaatt
    11641 cgccccatat tgccggaatt gccgccatga atcaactgcc aaacaaagtt actggacggc
    11701 ccaacccacg acggcacacc gggcggacct tgacttggac cgcctgcgga caaaactcct
    11761 ttggcctcca ctgccacggt tcaggttatg gtcgaagctg cccaagaact aatcacccgc
    11821 tacggctaca ttggcctgtt cggattgctg gcgctcggcg ttgtcggcgt acccgcgccg
    11881 gacgagacgc ttgtcgcggt ctccggactt ttgatcaaaa agggcgaaat gagccttgtg
    11941 ccgacattcc tggccgcggt cggcggcagc gccgtaggcg tcactatcag cttcgtggtc
    12001 ggccgcgtct tcggcttccg cctgatacac cggtacggcc atctgctgca cgtcacggaa
    12061 gagcgcctcg ggcgcttcca gcgctggtac gaacgtgtcg gacggtggac gctggttttt
    12121 ggctatttca taccgggcat tcgacaggtc acggctttcg cctccggaac gtccgggctg
    12181 tcgtgggcca gcttcgccgc attcgcctat tccggcgcgg tgctgtgggt cacgacgttc
    12241 cttacggccg gctatgttct cgaagcggag tgggagaaga gctcgccgag aatgcacgcg
    12301 gtaatcgtag gcgtggccgg cgtaggattc gcggtggtcg caacctacgg cgtgctgcgg
    12361 ctcatgcgct acagacgcaa cgtgacgccc gcctcgaatt cgacggcccc cgatgagtga
    12421 agccctcgcc gataattagg gacagtcacg tgactgtccc taattatcac gggcggtcgc
    12481 cgtttaggaa aaagggcaaa tccgcggcgt ctcgttcaac tctccccgcg gaatacagca
    12541 cgtagcgccc gccctcatcg acgccatcga tgttcacgct gtagagtacc gggcgcggcg
    12601 cgtgtggcac gtagccaatg ggccggtcat ccgcggcaaa cggatcgcct ggcacgctcg
    12661 gcagataatc gggaaccaac tcggccaggc gctctgggcg tcggccgtaa tcaagctcgt
    12721 atagtctgat cgccagcgcc gttgcggcca ttcgtcgctc agccagaccg tgcaagacca
    12781 cgtcctgcaa catatcatat ctcgacgcca gaatacttct ggggtagtgc gccatccggc
    12841 acgtcagcga atcggccccg ccgagcaaaa gcagattggc atcagccgag ggccaagtat
    12901 gcaacgcggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcaggaagat
    13021 catgtacgct atggctacgc aggtaatgcg cttgagccac cagaagcgcg gcggattgta
    13081 ggcgtcctgt tccatgctcg aaccaggcgt tagtgcgcgc gccgggcaga gcaacctcgc
    13141 ccaccgactc gctacagtat accgccgcta gccgacggcc ccggcattag cggctgtcga
    13201 cgcgctcagt atagttttat tcacgatcat ccggcatagt ttccacagaa gacgattgtg
    13261 aagccggcgc ggactgcgtc gcggccgact gcccggcaga tgcggacgcc gcaagcggcg
    13321 ggcgcgggcg gtcgccattg aggaagaaga ctaagtccgc cgcgtcgccg tctaccgcgc
    13381 cattcgttct gagctcgaag cgtccgccgt catccacgcc gtccaggttc agacagtaca
    13441 gcacgggcag agaagcgttc ggagcatatc caatgggccg gtcatcggcc gcatacggat
    13501 cgcgcggcac cgccggcagg tagtcggaca ccagttcctc cagacggtcg gggcgccgtc
    13561 cgtaatcaat ctcatacaga cgaatggcca gggcggtcgc ggccatacgg cgcgtagcga
    13621 gcgcccggaa cgaaagttgc accgcgcgct cgtaagaggg gagcaagatt gagctgagta
    13681 gatgcgccgt ttcatcgatt tcggaacggc ccgtgacctc ggcaggtatg tgtgcagcgg
    13741 ccgccggcca gtttgccgcg gccatcgcat tcgccatctg agttgtccat tcggccatga
    13801 aggccgtatc gagcacccaa gccggtccga gcaggagtaa gtgcggggcc agcgtgccca
    13861 gaatgggcga cccgctgaac aggccccagc gcccgttcat gaacgcgtgt gcatcctcta
    13921 tctgcgtcat gcgctcgaca tgcatagcgc gtaccgcggc ttgccggcat tccgcttcgt
    13981 cgagcaacgc ctcaatcagc gcgcttacct gggcacggct cgcggggccg gtcagcacgg
    14041 gatcaccagc gcctttttcc tctatgttga gagtgggagt aattcgttcc aacccgtcgt
    14101 aagccagggc gctgacgcca acactcacca gttgcgacat cagcggtccg tccgctaatg
    14161 cggccgcaat tcccaacata tcgttgcaag cctcaagcgc accggcgtcg tcgcccgcgt
    14221 tatgccgcgc gacaagcact accaattccc attcagaaaa aaggccgcgt tctcatcgtc
    14281 cggcaaaggc gcgataagga aatcgtccac cagcaccggc tcacccgccg cgcggcactt
    14341 cgcgatcgcg gcatcgaacc gtcgctgcgc ctcccagccc caccacaggc gaacggccag
    14401 caaaaccact agcatgatca cggcgtcgca cgccaggcgc ttgagccacc agaaccgcgc
    14461 cggtatcagc ttctcgttct gcgtcgcatc acccgatttc ggagacaccg aggccgacta
    14521 actggcggtt ctatacccag ttcagccgcc ccgcctccag tcaggcacgt aaagccccca
    14581 agtcccccta cgcgaggccc gctctgctat cgcataattt gcgtctgggc caaagcccga
    14641 gcggcattgt agacgatgcc cgcggggcca cccgaaatca cggcggcagg ctgaccttca
    14701 cgaccccctg cgtggacacc acgcgctttg ttagatactc gatgaacgtc gcgtgaaccg
    14761 tgatatccgg atgggccggc ggaccggacg gccatgggca tttgatcgtg tagtgattcg
    14821 tcatcagctt gccgtaccac aaatcgcggg cctgctcgac cggtatgacg tactgaccga
    14881 tcagattctc ggccggcgga ttcgccaggt cgtaaagctg aatccgaatc tcccccgcca
    14941 ccttcagaac gtcgcccgcc gcgtccacag gctgaagata gagcacgacg ccctcatcgc
    15001 ccggccggcc gtcgatgtca tatcctccgg agagcggggc aatcaccagc ttggtgggat
    15061 agaagacgtt ttgcagatcg tcattcgtga agccgcgcgc cgctgcgagc tgcttcgtca
    15121 gctcatcaat ctcggccttt tggcgggcga gctgctcgtt ttgcctgatg atgacttcgc
    15181 gctgggtttg aagctcgcgt tgcccggccg tatccggcgg cgaccagcaa gctgtaagga
    15241 acgcggcggc taacccgagc accagaatgc ccgcaacgcg ccaccgcaga aagatactcg
    15301 cgatcaactc cgcatcctcg tcctgcatcg gctcaagtcg ccgtttgccc tcgctagcgc
    15361 ttggggctcg gtcgccggcc gtcgctagcg cttcgggctc gggtcgccgg ccctcgctgg
    15421 cgctacgggc tcggattgtt ggcgctcatt ggcgcttcgg ggccgatcgc tggcggcatc
    15481 tggcgcttcg aatcggccta gctctgctcc ggcggcgtcg cgcgcagcgc cggcttgtcc
    15541 ggcatctgct ccagtatgct gtccgccagc ccgtacgcaa ccgtctgctc ggcgtccatc
    15601 cagttgttgc ggtcgcaatc ggccgcgatc tgctcgatcg gcttgccggt gtgcttggcc
    15661 agcacgcgat acaggtgttc ctgcgtccgc agcatctcgt cgagctcaat cttcaggtcg
    15721 gtggccggcc cgcgcgactg ccccaagggt tggtgaatca tggtccgcga gtgcggcagc
    15781 acgaaccgct tgcccggccg gccggccgcc gccagcaccg cccccatgct ggccgccacg
    15841 ccgatcacgt aagtcgcgac cgggcagggc acgaactgca tcgtgtcgta aatgcccatg
    15901 ccgctgtaaa ccgccccgcc gggtgaattt atgtacaggt gaatatccgc gttcttgtcc
    15961 tcgttcgcca ggaacagcat ctgggcgatc acgacattcg tcagctcggg cgtaatctca
    16021 tcgccgagga agatgatgcg gtccttcagc agccgcgagt agatgtcgta cgcgcgttcg
    16081 gcgcgtccct cgcgctcgat cacgatcgga atcaactggt tgttcatgaa acgcggcgct
    16141 cctggttatt tcttgctctt cttgggtttg ctttcctcga gaatctcgtc aatcagccca
    16201 tattccttgg cttcctccgc cgtcatgtac cggtcgcgct cgatgtcctt ggcgatttgc
    16261 tcggacgacc ggccggtgca gcgggcaaga atctcggtaa tcgtcttctt cactttcaga
    16321 atctcgtcgg cctgaatgcg catgtccgcc gcctggccgc ccatgtagcc ccacggctga
    16381 tggatcatga ccttggcgtg cggcagagcg tagcgcttgc ccttcgtgcc ggccgccaga
    16441 atcaaggccg ccccgctgct ggccgctccg acgcagtaag tcgcgatctc ggagttgagg
    16501 aactgcatcg tgtcgtagat cgccagcgtg tcgtcgatat cgccgccggg acagttgatg
    16561 tacaggtgaa tgtcctggtc gcgcttgatc gactgcaggt acaacatgct cttgatcacg
    16621 tgcgagcaga ccgtgctcgc attgatcgtc acgttcgggt cgagcggcag atccaagaaa
    16681 atgatgcggt tctcaagcag cagttcgttg aggcccatct gtcgctggta ttgcatctgc
    16741 gactgcatcc gcgggcgttc aactggcggc caatatgcca tgcggtctcc ttcggtatta
    16801 catcatactt atagcgccga gatcgcggct catcccggat tttttatcgg cctgatctta
    16861 tccgagcttc aggtgggctc ggccgccgac tccgcgccgg cggactcatg ctttacgccg
    16921 gtcaccttcg ccttctccag aagcatagac acggtcttgt cctgccggat tcgttcggcg
    16981 agctgcgaca gcagcccctg ctgctgtaac tcgtcgcgca cgcggtcgaa ccggcggttg
    17041 taacgccgcg ccatcgccgc gatggccgtg ttgacttcct cgtccgtaac ctcgatttcg
    17101 agcttttccg cggctttttc taggatgaac ccgagcttca gttcgcgcgt tacgtcctct
    17161 ttggccgagc tgcgcagcgc atccgcgcgc gccaaaacct cgtcctcagg cacgccgcgc
    17221 tgccgcaact cgatcatgcg ccgcaccacg gcccggtcaa tctgccgggc ggacagcttc
    17281 tccggcagct cgaggctcgt cttctcgagc aggaaagcct cgacctgggc gcgcttggcg
    17341 cgctcgatca tctcgtcgcg gtcgcgctcc atcatcatgc ggaagtgatc aaacgcgtcg
    17401 gcttccttgt cgaacccggc gcgctgcagg aagtcgtcca gcggcatcgg caccagccgc
    17461 ttcagctcgt gaatacggaa agtgaaccgc cccttctgac cgcgaatgtc agcccgctcg
    17521 taatcatccg gaatcgtgca ggtcgcctcg cgcgtctcgc ccggcttggc gccttccaac
    17581 accttgtcca gatcgggcac ctgcaccccg tcca
//
LOCUS       DAOSYS010000048        17156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2766_length_17156_cov_5.835682, whole genome shotgun sequence.
ACCESSION   DAOSYS010000048 DAOSYS010000000
VERSION     DAOSYS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 17156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17156
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2766_length_17156_cov_5.835682"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLU99_04645"
     CDS             complement(<1..557)
                     /locus_tag="PLU99_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOO16379.1"
                     /translation="MAPAVSDALGHGARAAPAVCRGRGRPAFDLGAGVLVSGHAVGFA
                     AVRPARLAGADVKRVMLVFGTRPEVIKLAPLVHAYRARASEVALTLCATGQHRQMLDS
                     TLAAFDMRADIDLNLMRGGQDSSEFLGRLILALRPVMNEVRPDIVCVQGDTATVMGAA
                     LTAFLCGAQVAHVEAGLRTRDKRAP"
     gene            743..889
                     /locus_tag="PLU99_04650"
     CDS             743..889
                     /locus_tag="PLU99_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16380.1"
                     /translation="MKLHKLALLLVSGGTLLSFAGCGRWALDLLYVLPQFVEFFQSLN
                     EATG"
     gene            complement(1194..2153)
                     /locus_tag="PLU99_04655"
     CDS             complement(1194..2153)
                     /locus_tag="PLU99_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO16381.1"
                     /translation="MNPARLVKRDLLDPRPESEAGLRPIPNAVNIPFAELGTRLHELP
                     PKSESIQVVGPPELATSVVEWLVQHGRVARVVAGQAEATAGTSPVRSEARANEVGRLW
                     EPSAFLEEVAAQLQLRAGESPVAPGSALDLACGSGRNAVFLACCGWKVTAVDILPDAL
                     DLGRALERRYARGRHAVNWICADIRTVDTGEMPQPNECTRGTPAQQQVLPQRVAPQQS
                     GWRPDEQFDLVTMFRYLDRGLVRQLPNWVNPGGSIVLETFTTEHRQRHGRPARAAHVL
                     RPGELRELFAPMLSRMWLRHYSEGWRGAEHTARLWAECSAPGS"
     gene            complement(2150..2293)
                     /locus_tag="PLU99_04660"
     CDS             complement(2150..2293)
                     /locus_tag="PLU99_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16382.1"
                     /translation="MSTLQGVARAVLTAALARGHRVLIVDSGNNRAQAIDGLARLRWR
                     SPE"
     gene            complement(2290..2688)
                     /locus_tag="PLU99_04665"
     CDS             complement(2290..2688)
                     /locus_tag="PLU99_04665"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO16383.1"
                     /translation="MLAPLLAACRPGVQWRLGPYEDARAQAQAQRQLNFVYFRTWYSV
                     ECTRFEEDVLMQPRVVEALRNLVAVPLEYDVDRDIARRWSITQVPAFAIVDPSGKVLA
                     SAQGVVSADVLLAAIERAQQKSAQLPAAAP"
     gene            3030..3962
                     /locus_tag="PLU99_04670"
     CDS             3030..3962
                     /locus_tag="PLU99_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO16384.1"
                     /translation="MPNVEQISPEELKRALPLLLATPGGRAASRAAVQVFQDYIRDPR
                     IQWDAWRIGSVDRPAALVLALLLPGSTAIVLVPVPGELGIRASEQQELLQAALARLCQ
                     RGLYFAQALILPTAHAQKDLLESAGFRLLAPLTYLERDVRYPWVDPPQVEESVWLTFG
                     RQTYDEFAALLLETYKESQDCPELTRLRPIDAVIAGHQAAGEFDPTLWEILRVDGVSA
                     GCILLAALRDGRTVELAYMGVAPAFRRRGLATVLLRRALALCRARSARRLTLAVDERN
                     EPAKRVYQRNGFQVTTQRDAYLFPFSSPGGLQAG"
     gene            4241..5647
                     /gene="dnaA"
                     /locus_tag="PLU99_04675"
     CDS             4241..5647
                     /gene="dnaA"
                     /locus_tag="PLU99_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOO16385.1"
                     /translation="MDTTAAERVCHIREKVARRIGMNRFQTWFGEPTAFSLEGEHLQI
                     MVENPLVGTWITANFLPQLIEATREVIGAEPSINVQVLKPRSDAAPALAPPAAATAQL
                     PPQRRPAAPAPRPRLLRGELENFVVGPSNQLAYSAAAAVVRRPGADFRLLVLHGACGL
                     GKTHLMQGICNGVRREHPDLEWRFVSGEQFTNEFVYAIKTGRIDAFRTRFRHVDVLLV
                     DDIHFLANKKGTQDEFLHTFDAIDGCGKAVILASDRHPRTIATLSEPLISRLVAGMVV
                     QINPPDFDTRRKILARRAIEINAQVSDEALDFIAHRIDGNVRELEGALYTLAAVAALA
                     KRPADLEMARRAVLDRADRAPDAADIQRVAATYFGLSREAIQSDSRDRTISLARAVTM
                     YLIRKHTRMSFPEIGRFLGNKNHSTVLMAVRRIEKMLEANGAVVWRTSAGPQESPLSE
                     LLATLENQLARGPGRRPN"
     gene            6049..6459
                     /locus_tag="PLU99_04680"
     CDS             6049..6459
                     /locus_tag="PLU99_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16386.1"
                     /translation="MRPRRAVMAKTTKKATRKPQRGRVLLLEPDHLTRWSITTYLCRW
                     FAVETVESSEDAARLVSDEPFDALVLSDRVSDGEVATLHSFAAGQNPRVKTVRTVTAA
                     SEPTTAQTSLVQLEKPFQLAQLAQLLGVAQPDLS"
     gene            complement(6655..8358)
                     /locus_tag="PLU99_04685"
     CDS             complement(6655..8358)
                     /locus_tag="PLU99_04685"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HOO16387.1"
                     /translation="MFDQPQWKKRPCPVQLTGRALLTCSLCNKGLAFSPEERDALGLR
                     GLLPARQLSIKEQVALEMEHLRAKETDLEKFIGLAALQDRNETLFYRVLVENTAELMP
                     IVYTPVVGQACQKYSHIFRQARGIWLTPEDRDRIPDVLRNWPERDIRLIVVTDNERIL
                     GLGDQGAGGMGIPVGKIALYCAGAGIHPSQCLPISLDVGTNNPDLLQDPYYIGYRQRR
                     LTGEAYDDFIEAFVMGVKKVFPRALVQWEDFLKNTAFAVLDRYRRRITCFNDDIQGTS
                     GVALAGMYAALRITGQKLCDQRIVYAGAGAAGVGIGRLVATAMAEETDNQAKIHKAQA
                     FLDSAGLVHEGQVIKDPHKRPFAMLRSEMREYGFTGDGPFDLLEVVKRVKPTILLGTT
                     ARPGTFTEEVVREMAKHCERPLIFAFSNPTSKAECTPEEAIRWSDGRALVATGSPFAP
                     VEYKGKRHVIGQGNNVFIFPGVGLGVILAEASEVPDLFFSVAAKTLADCITPDRLEQN
                     ALYPDQGRLREISAKIAVNVIREAKRLRIGRMIPDNEIEAYVAENMWYPEYSEYEYRP
                     G"
     gene            9241..11460
                     /locus_tag="PLU99_04690"
     CDS             9241..11460
                     /locus_tag="PLU99_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOO16388.1"
                     /translation="MVELTIFGRGGRGGVTLAKLIANAYFLRGKYAQAFGVYGAERSG
                     APVQAYVRIDDEEITNHNEIREPDHIIVIDPTLIAPSVASGMKPDGFVLLNTPQPPEK
                     FVEMFPGRRVATVDANEIAFANGLGSKTVPIVNTTLVGAFARLVDMPFSDVEAALAEA
                     HFGGPNVKAAKQAFDAIQSKKLSGKVEVQAAPRKKNRIAGILDAEVGGMPTIRTGTWA
                     SRRPSRHELTPACNNGCPAGNDVRGFVQAVARKEYDEALQLILKTSPFPGVCGRVCPA
                     PCMQACNRREHDESINIREIERYVADHALWPSATQPVRETEIAVVGSGPAGLSATYHL
                     ARLGYRVHLFEAGDEIGGVLRTGIPAYRLPRDVLDREISFILRHGVQTHTKRRIGRQE
                     LLDLTHKYAAVFVATGLQELRSLNLGNLAEDMVMQGIDFLDSARRGRISLTGQRVIVV
                     GGGNTAMDAARTALRVGARNVRVIYRRTRAEMPAIAEEIDEALEEGILLDELVNPLRL
                     HQDADGTVLTCQRMKLGPPDESGRPRPVPLETEDAVFEIRCDKVLLALGQSSDLTILP
                     EGSEVHVGKALAGLTGAPVFAGGDFATNDGTVTAAVGNGRRTALHIHRLLSGEDLFPP
                     APAPVAGPEAIMMQVFSHQPSERGRAIPPEVRRGSFSEVRLGLIDEPGHEAAWQEAQR
                     CFSCGVCNECDRCISYCPEGVMLHEGDRRYSFNFDYCKGCGICASQCPRGVIFMREL"
     gene            11470..12642
                     /gene="porA"
                     /locus_tag="PLU99_04695"
     CDS             11470..12642
                     /gene="porA"
                     /locus_tag="PLU99_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HOO16389.1"
                     /translation="MARALVTGNNAAGHTLAVAGEANRVARGCCGGAYPITPQTEIIE
                     FLRGFKFTKGEIVPVESEHSAMAVCIGASLAGARSFTASSSNGLTYMTENVFAAGFYR
                     LPIVMIAVNRTVGPPWNIWVDQGDSLALRDAAWLQFYCESHQDLVDTILVAFRVAEDR
                     RVMLPALVAEDGFILSHTTMVVDLPEQNLVDRYLPPCRVPHQLSFKHPETVGGLTWPR
                     ETEHHRREIQEAMEAVPAVMREALDEFEKIFGRRPHGAFTAEQTEDAEVVLVACNTMA
                     RTVRRVVEARRAKGEKVGLVKVKMFRPFLREEFAKAIGSAKRVGVLDRNHSPGSGGIF
                     WNEIATSLRQRPDVLLQDYLVGLGGGDVLPRHVEEILDDLKARKQSEEPIWKEVAA"
     gene            12716..13669
                     /locus_tag="PLU99_04700"
     CDS             12716..13669
                     /locus_tag="PLU99_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO16390.1"
                     /translation="MSARIDTPTNNSDFSAAKCEALTNQYVRSDGILRPGNTNCAGCG
                     MSLGLQWLDQGLEGEKPTMVIPACCGIVTAGGFPTSAYGVPTVATTFASSPAVATGIA
                     KVRGLNGETNPTVCWTGDGGTYDIGMATLSAAAERNENLIYICYDNEIYGNTGGQRSS
                     ATPLGVKTTTTVQGKAERKKDVMAIMAAHRIPYCATLSLAHREDFLRKIKKARQTRGF
                     RFLLILSPCPTGWKSEPTDSVELVRAAVGCGLFPLYEVFDGVRYRINARPDGTPLDRY
                     LAGQKRYKSESIDVGLIHSQIREQWACLEALERSFPARKED"
     gene            14052..14663
                     /locus_tag="PLU99_04705"
     CDS             14052..14663
                     /locus_tag="PLU99_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOO16391.1"
                     /translation="MAAALALAVCSYDQPITDRVVAARKSIETVLGPPAALATFLIFP
                     AILFSFANRRRLLIGFFSTVLLSGLITQTLKRVVGRARPDEHLGSVQFDLSKSLPSQD
                     IWLGMGQYHSFPSGDAMLALVLALLLGIYFPRARLVFYICAGFVGLQRIVLERHFASD
                     VLAGYAVAAASVYICLRLLGPTYYDRDLPESTCSLSPSEGGRE"
     gene            14668..15777
                     /locus_tag="PLU99_04710"
     CDS             14668..15777
                     /locus_tag="PLU99_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOO16392.1"
                     /translation="MSSLERSLCCLSVTLALACAAACGQPPAANPAPASMPAMLDVGA
                     PAPPIRAANWLNGKAVQIERQADSPVIVLTFVDTSAGPSRGALPALSALQERERQRGL
                     ICIAITEEPTAEVQKFIEHTRPPLTLRVAIDDQGATARAYCAAIGVNFRPYSFIVARD
                     GTIAWHGHPQQPELVQYVEQLLSGKYNPEQAREAVHQALTTEQLEAVFREAYANQAWG
                     TALLALDRLLEAGAPKDRLLRYKLAILLGEQRDQAAARALMAEVLKNYSANARLLNSL
                     AWDIVSTDRLYFQDPEIGLQLSQAAYRASGGQDPAVLDTYARALHLLGRLDLAIAVQE
                     KAVRLATEPDRQAFAQRLAFYRRCQELQAGLTAEP"
     gene            15952..>17156
                     /locus_tag="PLU99_04715"
     CDS             15952..>17156
                     /locus_tag="PLU99_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16393.1"
                     /translation="MKPRKQDFLLGLAVIIFLVLFLITVLFLARQRLGPTRPITVIFP
                     HAEPMAPVTEGSDVLLSGALKVGQVTAVRFETTSVESPRGSQPSLVIAVDAEIVQALP
                     LYGDCRISTDMPLIGGTGTLVIQFVGTPGVPLPPGPIHGLPSEGAAAFANITRRLSEP
                     GGIIDRVDQMLNPDLEGSLMNRLITTLADVNVITGELRLQLTVEERDTLLGKMQAILD
                     ELQEMSTALSDQAQAAVPGSIMAKLSSALDLVHADLQMVLEMIRDSQPLVHDALDNLR
                     HATRVIDQDVVGTLAAQMDLANSASLLAKAHSAMEDVNSVTANFKSVSELVRNLLVVD
                     GQKVSIILDNLQEMSAQLSATSRELRLNPARLIWGPSRGEQKRIAAFQAARDFAQAAE
                     SLDQAATDLR"
BASE COUNT         3125 a         5583 c         5357 g         3091 t
ORIGIN      
        1 aacggggccc gcttgtcacg cgttcgcagg cccgcctcca cgtgcgccac ctgtgcgccg
       61 cacagaaagg ccgttaacgc agcgcccata accgtagccg tgtcgccttg tacgcacacg
      121 atgtccggac gcacttcgtt cataaccggc cgcagcgcca ggatcagccg cccgaggaac
      181 tcgctggaat cctgcccgcc ccgcatgagg ttcagatcga tgtccgcccg catgtcgaac
      241 gcggccagtg tgctgtccag catctggcgg tgctgaccgg tggcgcacag ggtcaacgcg
      301 acctcgctgg cacgcgcgcg gtaggcgtgc accaggggcg caagcttgat gacctccggc
      361 cgcgtgccga aaacaagcat tacgcgcttc acgtccgctc ctgccagccg cgccggccga
      421 accgctgcaa aacccaccgc gtggccggat actagcacgc ccgccccgag gtcgaaagcc
      481 ggccgcccgc ggccgcggca caccgccggc gcagcacggg cgccatgccc aagcgcatcg
      541 ctgacggcgg gtgccatgcc caagctgtaa gcgcgggcat gccctcgctg tgtccgttgt
      601 acgaccgcac tgacggtccg agtccaccga agccggccag cagccccggc gcggcgcgca
      661 gcattccact tttgcagccc cacatccacc gttattatcc ccgcattacc ggcggcctgg
      721 ctgccaactg aaggaggacg tgatgaagtt gcacaaactc gcgctcttgc tggtctcggg
      781 tggaacactc ttgtcgttcg ccggttgcgg acgctgggcg cttgacttgc tctacgtcct
      841 gccccaattc gtcgagttct tccagtcgct gaatgaggct accggataaa gcctcactgc
      901 accgcccgca cccaggccgc ccgatgtgaa ccgcccgcgc gcaggcttcg cgtgtgtcac
      961 tgcttgaagc cgaagcttca agcggtgcac tccgacgcca gaggcgcgtg ttcttcggcg
     1021 aaaccgaccc cttgtggtgg atgcccacac cgccgagtgg ggggttacct tcgcgacttt
     1081 tgctttacgc cagaggctgc gtgtctagtg cggcgcagat agtaagaagt gcacactgtc
     1141 tagtgtatat gtagatttca gggctgaggc gccatgccgc actgaagagc ctgttagctg
     1201 ccgggcgcgc tgcactccgc ccataagcgt gccgtgtgct ccgcgccacg ccagccctcc
     1261 gagtagtgcc gtagccacat gcggctcaac atcggcgcaa aaagctctcg cagctcaccc
     1321 ggtcgcagaa cgtgcgcggc acgcgcgggc cgcccgtgcc gttggcggtg ctcggtcgtg
     1381 aacgtctcca gcacgatact cccgcccgga ttgacccaat tcggcaactg tcgcacgagg
     1441 ccccgatcca ggtaacgaaa catcgtaacc aaatcgaatt gctcatccgg ccgccacccc
     1501 gattgctgtg gcgccactcg ctgtggcaac acttgctgtt gcgccggcgt cccgcgcgtg
     1561 cactcattgg gctgcggcat ctcgccggtg tcgacggtac ggatgtccgc acaaatccaa
     1621 ttaaccgcat gccggcctct cgcataccgc cgctccagcg cgcgccccag atcaagagcg
     1681 tccggcagaa tgtcaaccgc cgttaccttc cagccgcaac aagccaggaa cacggcgttc
     1741 cgccccgacc cacacgccaa gtcgagagcg ctgcccggcg ctaccggcga ctcgcccgcg
     1801 cggagctgaa gctgcgccgc aacttcctcc aaaaacgcgc tcggctccca caaccggccg
     1861 acctcattgg ctcgcgcctc ggacctcacc ggcgaagtgc ccgcagttgc ttccgcctgc
     1921 ccggcgacaa cccgcgcgac acgcccgtgc tgcaccagcc actccacgac actcgttgcc
     1981 aactccggcg gcccaacgac ctgaatactc tccgacttgg gcggcagctc gtgcagccgc
     2041 gtgcccagct ccgcgaaagg aatgttcact gcattgggaa tcggccgcag cccggcctcg
     2101 ctttccggcc gcggatcgag caggtcacgc tttacaagcc gcgctggatt cactcagggc
     2161 tgcgccacct caagcgcgcc aggccgtcta tcgcctgcgc gcgattgttg ccggaatcca
     2221 cgatcagcac ccggtgaccg cgcgcgagcg cggccgtcag aaccgcgcga gcgacgccct
     2281 gcaacgtgct catggcgcgg cggcgggaag ctgtgcgctc ttctgctgcg cccgctcaat
     2341 tgccgccagc agcacgtccg ccgaaacgac tccctgggcc gaagcgagca ctttccccga
     2401 tggatcgacg attgcgaatg ccggaacctg tgtgatgctc cagcgccgcg cgatatcgcg
     2461 atccacatca tactccagcg gcacggccac caggttccgg agcgcttcta ccacgcgcgg
     2521 ctgcatcagc acgtcttcct caaaccgcgt gcactcaacc gagtaccacg tgcggaagta
     2581 cacaaagttc aactgccgct gcgcttgcgc ctgcgcgcga gcgtcttcat aaggtccgag
     2641 tcgccattgc accccgggcc ggcacgcggc cagcaggggc gcgagcaaga acactccggc
     2701 tacgacggtc agcggccttg gtcgccaaca cgcttgacac acgctccgca tggaaatggg
     2761 cggcctccgt tcttttgcgg ctcatacttc agcgccgccg cagggcgtca caggcgacat
     2821 ttgcttcgcg acacgccgtt cggcacaatg gcgcacttgc tcgccggaag acgcgaaatt
     2881 gtaagcgtcg cccgcggctt cgccaagacg cccaacggcg ggcggccaag gcgcaataaa
     2941 acgggccgcg ccagccctgg ggcgccgcta aaactttcta ccggccgccc tcacgtggcc
     3001 gccgatgcag cagtacacgc aggaccgtcg tgccaaacgt cgaacagatc agccccgaag
     3061 aactgaagcg ggcgctgccg ctgctgctcg ccacgcccgg cgggcgcgcc gcttcgcgcg
     3121 cggccgtgca ggtcttccaa gattacatcc gcgatccgcg aatccagtgg gacgcgtggc
     3181 gtatcgggag cgtggatcgg cccgcggctt tggtattggc tctcttattg cccggttcga
     3241 cggcgatcgt actggtaccg gttccgggcg aactgggtat cagggctagc gagcaacaag
     3301 agctgctgca agctgcgctg gcgcgcctgt gtcagcgcgg actttacttc gcgcaggctt
     3361 tgattctgcc aaccgcgcac gctcaaaagg acttattaga aagcgccggc ttccgcctgc
     3421 tcgcgccttt gacttatcta gagcgcgacg tgcgatatcc atgggttgat ccgccgcagg
     3481 ttgaagaatc agtctggctg acgttcggtc gtcagacgta cgacgagttc gcagcattgt
     3541 tgcttgagac atataaggaa agccaggatt gtcccgaact aacgcggttg cgtccgatcg
     3601 acgcggtcat cgcgggacat caggcggccg gcgagtttga tcccacgctt tgggaaattt
     3661 tgcgcgtgga tggcgtgagc gccggctgca ttctgttggc ggcgctgcgc gacggacgga
     3721 cggtcgagct cgcatatatg ggtgtagcgc cggcgttcag gcggcgtggg ctcgccacgg
     3781 tgctgctgcg gcgcgcgctg gcgctgtgcc gtgcgcgcag cgcgcgccgt ttgacgctcg
     3841 cggttgacga gcgtaacgag cccgccaagc gtgtgtacca gcggaacggg tttcaagtca
     3901 ccacgcaacg tgatgcatac ctttttcctt tttcgagtcc cggtggtttg caagccggct
     3961 gatggcgctg cgcgtcgcgc cacgcggcgc ggcggcaatt gttgaaaacc ggtgcttcta
     4021 agatgcgcca cgaaaatttt tttctgcaac agtttgtggt gtgtccagta gttacgttgc
     4081 gccgcgcatc tgttgataaa aacttgattg ctaaatcctt aagcgcgtgt tatatttcaa
     4141 ctgccgtcgt tcctggtggg gatgggcggc gtgttcggcg attctcttac aaacacaaat
     4201 aacgctcagc ttttgctggg atgttttctt gaggcggatg gtggatacaa ccgcagcgga
     4261 gagggtttgt catattcgcg agaaggtcgc gcggcgcatc gggatgaacc gcttccagac
     4321 gtggttcggt gagcccacgg cgttttccct tgagggcgag cacctgcaaa taatggtgga
     4381 gaacccgctc gtcggtacgt ggattaccgc gaacttcttg ccgcaactga tcgaagccac
     4441 gcgcgaagta atcggggccg agccgtcaat caacgtgcag gtcttgaagc cgcgctccga
     4501 cgccgcgccg gcgttggcgc cacctgccgc tgctacggct caactgccgc cgcagcgccg
     4561 gcccgcagcg cctgcgccgc gtccgcgcct gctgcgcggt gagctcgaga acttcgtagt
     4621 cggaccctcc aaccaacttg cgtactctgc cgctgccgcc gttgtgcgac gtcccggggc
     4681 ggatttccgt ctcctggttt tgcacggcgc ttgcgggctc ggcaaaacgc acctgatgca
     4741 gggcatctgc aatggcgtgc ggcgcgagca tccggatttg gagtggcgct tcgtctcagg
     4801 cgagcaattc actaatgagt tcgtctacgc catcaagacc ggacgcatcg atgcgttccg
     4861 tacgcggttc cggcacgtcg atgtgcttct cgtcgacgac atccacttcc tcgccaacaa
     4921 gaaaggcacg caagacgaat tcttgcatac tttcgatgcg atcgacggct gcgggaaagc
     4981 cgtcatactc gcttccgatc gccacccgcg aacgatcgcg acgctttcgg agccgctaat
     5041 cagccgcctc gtggccggca tggtggttca aatcaaccca cccgacttcg acacgcgccg
     5101 caaaatcctg gcgcgtcgcg ccatcgaaat caatgcccag gtctccgacg aggcgctcga
     5161 cttcattgcc cacagaatcg acggcaacgt gcgcgaactc gaaggggcct tgtacacgct
     5221 ggccgccgtg gctgctttgg ccaagcggcc cgccgatctc gagatggccc gtagagcggt
     5281 tttggaccgg gccgaccgtg cccctgatgc ggccgacatc cagcgcgtcg cggccaccta
     5341 cttcggcctt tcgcgtgagg ccattcagtc cgattcgcgc gaccgcacga tcagcttggc
     5401 gcgcgctgtg acgatgtacc tcatccgcaa acacacgcgc atgagctttc ccgagatcgg
     5461 acgctttcta ggtaacaaga accactcgac cgtactcatg gccgtgcgcc gtatcgagaa
     5521 aatgttggaa gcgaatggcg cggtcgtgtg gaggacgtca gcgggaccgc aggagtcgcc
     5581 cctgtctgag ttgctggcca cgctcgagaa ccaactcgcg cgcgggcctg gccggcgacc
     5641 aaattagagg cgaccgctcc gcgggcgtca gcggcggtgg cacggatgcc gccgccgttc
     5701 ctgttcgcga ctaagccacc tccgcccgac gtgtcaatcc tcactcctct ccctcaactt
     5761 caagactatg accagccgaa atgaggaata tgcctcataa gcgcggcttt tggcacaaag
     5821 gcaacaggtg cgcgggcgca cgccggtcat aaccccctga aaatgcagta acttgtggca
     5881 ttctgcacag atcgtcgtcg ccccgtcccc cgagatccca cgcgcccccc ggatccgagc
     5941 cctgcatgcc gcagagaaca acgcagcccg cgaactacca ggcattcgat cgagaggcga
     6001 acaaaaatgg cacgcagcat gctgacccgc tgtccccagg agcttctgat gaggccgaga
     6061 cgcgccgtca tggcgaaaac cacgaaaaaa gccacacgaa agccccagcg cgggcgcgtg
     6121 ctgctgctcg agccggacca cctcacgcgc tggtcgatca ccacgtacct gtgccggtgg
     6181 ttcgcggtcg agacggtcga gtcgagcgaa gacgctgcgc ggcttgtgtc agatgagccc
     6241 ttcgatgcgc tcgtgctttc ggaccgcgta tctgatgggg aagtcgcaac tctccacagt
     6301 ttcgccgccg gccaaaaccc acgcgtcaag accgtacgaa cggtgacggc tgcctcggag
     6361 ccaaccaccg cccagacgtc attggttcag ctcgagaagc cgtttcaatt agctcaactc
     6421 gcgcaattgc tcggagtagc acaacccgac ttgtcgtgag ctattcggca tgcggaccac
     6481 gtagccgcgc tccctctgcc gcactgtgat ccgctgaatc cctgcgctcg gccggaagcc
     6541 tggcccccct tacaaaaaaa cccgccccag ggtgcacaat aacacccggg gcgggcgcac
     6601 tccaatgggt aggagacggt ccgcgctccc gccgcagcgg gggccgcacc gcgcctatcc
     6661 agggcgatac tcgtactcgc tgtattcagg ataccacata ttttccgcca cgtaagcttc
     6721 aatttcgttg tccgggatca tgcggccgat ccgcagccgc ttggcctcgc ggatgacgtt
     6781 gacggcgatc ttggcgctga tctcgcgcag gcggccctga tccgggtaaa gcgcgttctg
     6841 ctccaggcgg tcaggcgtaa tgcaatctgc cagagtcttg gccgcaaccg agaagaacag
     6901 gtcgggcact tcgctcgctt ccgccagaat cacgcccagc ccgacgcccg ggaaaatgaa
     6961 aacgttgttg ccctgcccga tcacgtgccg cttgcccttg tactcgaccg gcgcgaaggg
     7021 gctgccggtc gctaccaggg cccgcccgtc agaccagcgg atagcctctt caggcgtgca
     7081 ctcggccttc gacgtcggat tgctgaacgc gaaaatcagc ggccgttcgc aatgcttggc
     7141 catctcgcga acaacctctt ccgtgaaggt gcccgggcgg gcggtcgtgc ccagcagaat
     7201 ggtcggcttc acccgcttga ctacttcaag cagatcgaac gggccgtcgc ctgtaaagcc
     7261 gtactcgcgc atttcgctgc gcaacatggc gaaggggcgt ttgtgcgggt ctttgatgac
     7321 ctggccttcg tgaaccaggc cggccgaatc caggaacgcc tgcgccttat gaatcttcgc
     7381 ctggttgtcg gtctcctcag ccatcgccgt ggcgaccagg cgcccgattc caacgccggc
     7441 cgcgccggca ccagcgtaga caattcgctg atcgcacagc ttctgaccgg tgatgcgcaa
     7501 ggcggcatac attccggcca aggccacgcc ggaggtgccc tgaatgtcgt cattgaagca
     7561 cgtgatgcgg cggcgatacc gatcgaggac ggcgaaggcg gtgttcttga ggaagtcctc
     7621 ccactgcacg agggcccgcg ggaacacttt cttgacgccc atcacgaagg cttcgatgaa
     7681 atcatcgtac gcctcgccgg tcagccgccg ctgccggtag cctatgtaat atggatcctg
     7741 tagcagatcc ggattgttag tgcccacgtc gaggctgatc ggcaggcact gcgacgggtg
     7801 aatgccggcc cccgcgcaat acaaggcgat cttgccgacg ggaataccca tgccgccggc
     7861 gccttggtcg cccagcccca gaatgcgttc gttgtcggtc acgacaatca gccggatgtc
     7921 gcgctcgggc cagttccgca gcacgtccgg gatacggtcg cgatcctcgg gcgtgagcca
     7981 tatcccgcgc gcctggcgga aaatgtggct atatttctgg cacgcctgtc cgaccaccgg
     8041 cgtatagacg atcggcatca attcggcggt gttctcaacc aggacgcgat agaaaagggt
     8101 ctcgtttcga tcctgaaggg ccgccaggcc gatgaacttc tcgagatcgg tctctttagc
     8161 gcgcaggtgc tccatttcca gggctacctg ctccttgatg gagagttggc gggccggcag
     8221 caggccgcgc aggcccaacg cgtcgcgctc ctcgggcgag aatgccagcc ctttgttgca
     8281 caaagagcaa gttagcaggg cccgcccggt cagttgcacg ggacaaggcc gtttcttcca
     8341 ctggggttga tcaaacatcg caggactcct tacgtctctg atgcgggtct ggcgtcacgc
     8401 gcccgggaca caaaacctaa acgcggcccg gcagcaaaac ccatgccacc ggtgcgcagc
     8461 accggcttga cccagccggc cgggtcgggc gcgaccccgg tagtgtaacg gccggccggt
     8521 ccgaatgcag ccaaagaggg gagatgcggg tgctcagact tcggggtatg tcttgtggtg
     8581 gaagcagggt gccggtgctc aacctcgttt gattggcaag atagtcgaag tagtccagac
     8641 cacttgccgg ccctcaacgc ggcggtcgag ccggtgagcg accacggcaa gggctttgcg
     8701 gtcgtcgccc actcagcaaa tacccgcggc ttggcaagcg caggctccgt gcaggcgcca
     8761 acgaagcatg ctggccgagt gacgcgccgc cgaatccgac ggccccgatt tgaattctcc
     8821 caaacctgaa cgcgggcgag gcgcccgcgt gagcgcaaac gcgcccgcga gttgcgacgg
     8881 cattttgctc ctcacggctg gcgaggccgc ccggtaatga gtgcatgttc acacaatccc
     8941 agtggccaaa tgcgctcacc ggacttatag tgaagcctcg aaagcgctcc cggaatggag
     9001 ctcacagcat tgacgcctgt gagttcgcga gattatcaga cgtggcgacg ggaacgaatc
     9061 ttgcccaacc aacccgttgg caggccgcga ttgtttcggt atgaaaccga cgcgagccgc
     9121 ggctatcggt agctggtctg tttgggaatc caggcgtgcc gcggggctcg tgtgctccgg
     9181 cgggccgcgc cgggcagaga accgctggcc cgccgctgcg ggccgctttc cgggtgcggc
     9241 atggtggaat tgacaatctt cggccgcggc ggcaggggcg gtgtcaccct cgccaaacta
     9301 attgccaacg cctacttctt acgcggtaag tatgctcagg ccttcggcgt ctacggcgcc
     9361 gagcggtccg gtgcaccggt ccaggcttac gttcggatcg acgatgagga aataaccaac
     9421 cacaacgaaa tccgcgagcc ggatcacatc atcgtcatcg atccaacgct gatcgcgcct
     9481 tcagtggcgt ccggcatgaa gcccgacggc tttgtgttac tgaacactcc gcagccgccg
     9541 gagaaattcg tcgagatgtt ccccgggcgc cgcgtagcca cggtagatgc caacgaaatc
     9601 gccttcgcga acgggctcgg ttccaaaacc gttccgattg tgaatacgac cctcgtcggc
     9661 gctttcgccc ggctggtaga catgccgttc agcgacgtgg aagcggctct ggccgaggcg
     9721 catttcggcg gcccgaacgt caaggcagcc aagcaggcgt tcgacgcgat acagtcgaaa
     9781 aagctgtccg gcaaggttga agtgcaagcc gcaccgcgaa agaagaaccg catagcggga
     9841 atcttggacg cggaagtcgg cggcatgccg accattcgta ccggcacgtg ggcttcacgg
     9901 cgtccaagcc gtcatgagct caccccggcc tgcaacaacg gttgtcccgc cggcaacgac
     9961 gtgcgcggtt tcgtgcaagc cgtcgcccgc aaagagtacg acgaggcgct gcaactgata
    10021 ctgaagacgt cgcctttccc gggcgtatgc ggacgtgtct gccccgcgcc gtgcatgcaa
    10081 gcttgcaatc ggcgcgagca cgacgagtcc atcaatatcc gggagatcga acgctacgtc
    10141 gccgaccatg ccttgtggcc cagcgccacg caaccggtgc gcgaaacgga aatcgcggtt
    10201 gtcggctccg gtccggcagg tctcagtgcg acgtatcacc tcgcccgcct cggataccgc
    10261 gtccaccttt tcgaggccgg cgatgagatc ggcggcgtgc tgcgaaccgg cattcccgcc
    10321 taccgcctgc cgcgcgacgt gctcgaccgg gagatcagct tcatactgcg gcatggcgtg
    10381 cagacgcata ccaagcgccg catcggtcgc caagaactgc tcgacctgac gcacaagtac
    10441 gcggccgtgt ttgtggccac cggcctgcaa gaactgcggt cgctgaacct cggcaatttg
    10501 gccgaggaca tggtgatgca gggcatcgat ttcctggaca gtgcgcggcg cgggcggatc
    10561 agtctcaccg ggcagcgggt aatcgtagtg ggcggcggga acaccgcgat ggatgccgcg
    10621 cgcacggcgc tgcgcgtcgg cgcacgcaat gtacgggtca tctatcgccg cacgcgggcg
    10681 gaaatgccgg ccatcgcgga agaaatcgac gaggcgctgg aagagggcat tttgctggat
    10741 gagctggtca accccctgcg gctgcaccag gatgcggacg gaacggtgct gacgtgccag
    10801 cgcatgaaac tggggccgcc ggatgagtcg ggccgcccgc gaccggtccc gctcgagacc
    10861 gaggacgcgg tattcgaaat ccggtgcgac aaggtactgc tggccctcgg tcagagcagc
    10921 gatctgacaa tcctgccgga gggttctgag gtccacgtgg gcaaggccct agccggactg
    10981 accggcgcgc cggtgttcgc cggcggtgac tttgccacca atgatggcac ggtcaccgcg
    11041 gcggtgggca atggccgccg aaccgcgctg cacattcacc gtctgctgag cggtgaagat
    11101 cttttcccgc cggcgcctgc tccggtggcc ggccccgaag ccatcatgat gcaggtgttc
    11161 tcgcatcagc cgagcgagcg cgggcgggcg attccgccgg aggtgcggcg cggcagcttc
    11221 agcgaggtgc gcctgggtct gatcgacgag ccggggcatg aagccgcctg gcaggaagcg
    11281 cagcgctgct tcagttgcgg cgtctgcaac gagtgtgacc gctgcatcag ctattgcccg
    11341 gagggggtga tgctgcacga gggcgaccgc cgttacagct tcaacttcga ctactgcaag
    11401 ggctgcggga tttgtgcctc gcaatgcccg cgcggcgtca ttttcatgcg agaactctag
    11461 gaggccgaaa tggcacgcgc actggttacc ggaaacaacg ccgccgggca cacactggcc
    11521 gtcgccggcg aggcgaaccg ggtcgcccgc ggctgttgcg gcggggctta tccgatcacg
    11581 ccgcagaccg agatcattga gttcttgcgc ggtttcaagt tcaccaaggg tgagattgtc
    11641 ccggtcgaat ccgagcacag cgccatggcg gtgtgcatcg gtgcctcgct ggccggggcc
    11701 cgctcgttca cggccagctc ctccaacggg ctgacgtaca tgacggagaa cgtcttcgcg
    11761 gcgggctttt accggctgcc gatcgtgatg atcgcggtga atcgcacggt cgggccgccc
    11821 tggaacatct gggtcgatca gggcgacagc ctggcgttgc gtgacgcggc ctggttgcag
    11881 ttctactgcg agtcgcacca ggacctggtg gatacgattc tggtcgcatt ccgcgtggcg
    11941 gaggaccggc gggtgatgct gccggcgctg gtcgctgagg acggtttcat tctgtcgcac
    12001 acgaccatgg tggttgatct gccggagcag aacctggtcg accggtatct gccgccgtgc
    12061 cgcgtgccgc accaactgag cttcaagcac cccgagacgg tgggcgggct gacctggccg
    12121 cgtgagaccg agcatcatcg gcgcgaaatt caggaggcca tggaggcggt gccggcggtg
    12181 atgcgggaag ctctggacga gtttgaaaag attttcggcc ggcgcccgca tggggccttt
    12241 acggccgagc agaccgaaga cgccgaggtg gtgctggtgg cctgcaacac gatggcccgc
    12301 accgtgcggc gggtggtcga agcgcggcgt gccaagggcg agaaggtcgg cctggtgaag
    12361 gtgaagatgt tccggccttt cttgcgggag gagttcgcca aggcgatcgg ctcggccaag
    12421 cgtgtgggag tgctcgatcg caaccactcg ccgggttcgg gcggcatctt ctggaatgag
    12481 atcgccacca gtctgcggca gcggcctgat gtgctgcttc aagattacct ggtcggcctg
    12541 ggcggcggag acgtgttgcc gcggcacgtt gaggagattc tggacgatct caaggcccgc
    12601 aagcagtccg aggagccgat ctggaaagag gtggcggcat gagggatcgt cagtcatcag
    12661 ttgtcagttg tcagtctctc cgagggccgg cgggccgggg gagcgagggc atagtatgag
    12721 cgcccgcata gatacaccga cgaataactc cgacttcagc gcggccaaat gtgaagccct
    12781 gacgaatcag tacgtccgct cagacggcat tctccggccc ggcaacacga attgtgccgg
    12841 gtgcggcatg tcactcggcc tgcaatggct cgaccaaggc ctcgaaggcg agaagcccac
    12901 gatggtcatt ccggcctgtt gcggcatcgt gactgccggc ggttttccaa ccagcgccta
    12961 cggcgtgccc acggtcgcca ctacgtttgc cagttcgccc gcggtagcca ccggcattgc
    13021 caaagtccgc gggttaaatg gcgaaactaa tccaaccgtg tgttggaccg gcgacggcgg
    13081 aacgtacgac atcggcatgg ctacgctctc ggccgcggct gagcgaaacg aaaatctcat
    13141 ctacatttgc tacgacaacg aaatctacgg caacaccggc ggtcaacgca gtagcgccac
    13201 gccgctgggc gtcaagacta cgacgaccgt acaagggaaa gccgagcgca agaaggacgt
    13261 gatggccatc atggcagccc atcgcatacc ttactgcgcc acgctgtcgc tggcgcatcg
    13321 cgaggatttc ctgcgcaaga tcaagaaggc ccgccaaacg cgcggttttc gctttctgct
    13381 gattctgtca ccttgcccga cgggctggaa gtcggagccg acggatagtg tggagttggt
    13441 ccgggcggcg gtaggttgtg gactcttccc gctgtacgag gtgtttgacg gagtccgcta
    13501 tcgcatcaac gcccggcctg acggtacgcc gctggaccga tacctggcgg ggcaaaagcg
    13561 ctacaagtcc gaatcgatcg acgtgggcct gattcacagc caaattcgcg agcaatgggc
    13621 ttgcctcgaa gccttggaaa ggtcgttccc ggcccgaaaa gaagattaag gcccgtagtc
    13681 ttgcgtgaca tgcgcgcggc atgctgaagt gaaccgccac gcgcctgtga gcaacatcct
    13741 gcccgattga aacgaggtac agcacggctc gaatgaggcg agcctccgcg cctgcgaaaa
    13801 ctcggcaggc cgccgttccg accccttctt tcttcgatac ctttacggtg ttctgggggc
    13861 cgtctacact cctgcttcag ttggtggcaa gaacaggcgt gcgcggagtc actgcaatca
    13921 agatggaaaa acaggcaccg attcgttcgc aagtggccgg ccatgccagg ccccaaccgt
    13981 ccgacgaccc gagcggccag tcatcccgaa ttcagcgacc ggcgcctgca cggttgttgt
    14041 ggctgatttt ggtggcagca gctctcgcgc tggccgtctg ctcttacgac cagcctatca
    14101 ccgaccgcgt ggtcgccgcg cggaagagca tcgagaccgt actcggcccg ccggccgctc
    14161 tggccacgtt tctgatattc ccggcgatcc tgttctcttt tgccaaccgg cggcgactgc
    14221 taatcggatt tttttcgacg gtgcttctct ccggcctgat tacccaaacg ctcaagcgtg
    14281 tggttggccg cgcgcggccc gatgagcacc tcgggtcagt tcagttcgac ctgtccaagt
    14341 cgcttccgtc gcaggatata tggctgggca tgggccaata tcactcgttc ccgtccgggg
    14401 acgcgatgct ggctttagtg ctggcgctgc tgctggggat ttatttcccg cgggcgcgct
    14461 tggttttcta catctgtgcg ggcttcgtcg gcctgcagcg catcgtgctg gagcgacact
    14521 tcgcctccga cgtgctggcc ggctacgccg tagcggcggc gtccgtatac atctgcctcc
    14581 ggctgctggg gccgacgtac tacgatcgcg atttgcccga aagcacgtgc tctctttcgc
    14641 cgtcggaggg cggccgggag tgatcgaatg agcagcctcg agcgaagttt gtgctgtctg
    14701 agcgtcaccc tggcactcgc ttgcgccgcc gcttgcgggc aaccgcccgc cgcgaacccg
    14761 gccccggcca gcatgccggc catgctggac gttggcgcgc ctgcccctcc gattcgggcc
    14821 gcaaactggc tcaatggcaa agccgtgcag attgagcggc aggccgattc gcccgtcatc
    14881 gtgttaacct tcgttgacac gagtgccggg cccagccgcg gcgcgctgcc ggcgctcagc
    14941 gcgctgcagg agcgcgagcg gcagcggggc ctgatctgca tcgccataac ggaagaacct
    15001 accgccgaag tgcagaagtt tatcgagcat acccgccccc cgctgacact ccgggtcgcc
    15061 atcgacgatc agggcgccac ggcccgcgcg tactgcgccg cgatcggcgt taacttccgg
    15121 ccctacagct tcatcgtggc gcgcgacggc acgatcgcct ggcacggcca tccgcagcag
    15181 cccgagctcg tgcagtacgt cgaacaattg ctctcaggca agtacaaccc ggagcaggcc
    15241 cgcgaggccg tacatcaggc gctcactacc gaacagctcg aagcagtctt ccgggaagcc
    15301 tatgccaatc aggcgtgggg cacggctctt ttggccttgg atcgcctgct cgaagcgggc
    15361 gcccctaagg accgcctgct gcgctacaag ctcgcgatcc tgctgggcga gcaacgcgac
    15421 caggccgccg cccgcgccct gatggccgaa gtgctgaaga actacagcgc caacgcgcgt
    15481 ctgctcaaca gcctggcctg ggacatcgtc agcaccgatc ggctctattt ccaggatccg
    15541 gaaattggac tccaactgtc ccaggccgcc taccgcgcct cgggcggtca ggaccccgcc
    15601 gttctggaca cctatgctcg ggccctgcat ctgctcggcc ggctggacct ggcaatcgcg
    15661 gtccaggaga aagcggtgcg cctggctact gaacccgatc gccaggcatt cgcgcagcgg
    15721 ctggcctttt accgtcgctg ccaggaattg caggcgggcc tcacggccga accgtagcag
    15781 cagcggcccg tcactgatcg atctgtgcgg cactgccaaa ctcgttcgcc tcaaaggccg
    15841 gccagcccta caatgcggct gctgcgcagg gtattggcag cctccggatg gtgtgcgcgt
    15901 cggctcagcg ctgggccggg cgaaatcagt ttgcttgtcg agtgagtggc gatgaaaccg
    15961 cgcaagcagg atttcttgct cggattggcc gtcatcatct tcctggttct atttctgatt
    16021 accgtgcttt tcctggcacg gcagaggctg ggcccgacac gcccgatcac cgtgatcttt
    16081 ccgcacgctg aaccgatggc cccggtgacc gaaggcagcg acgtcttgct gagtggagcg
    16141 ttgaaagtcg gtcaagtcac cgcggtgcgc tttgaaacga ccagcgtcga atcgccgcgc
    16201 ggctcccagc ccagcctggt catcgcggtt gacgcggaga ttgtgcaagc gctcccacta
    16261 tatggcgatt gcagaatttc gaccgatatg ccgctcatcg gcggcacggg cacgctcgtt
    16321 attcagttcg tgggcacgcc gggggtgcct ttgccgcccg gacccattca cggcttgccc
    16381 tcagaaggcg cggccgcgtt tgccaacatt acgcgccgcc tgtccgagcc gggcgggatc
    16441 atcgatcgcg tggatcagat gctcaatccc gacctggaag gctcgctgat gaatcgcctg
    16501 atcacgacgt tggcggacgt gaatgtcatc accggcgagc tgcggctgca actgactgtc
    16561 gaggagcggg atacgcttct aggcaagatg caggcgatac tggacgaact gcaggagatg
    16621 tcgaccgccc tcagcgacca ggcgcaagcg gccgtccctg gttcaatcat ggccaaactg
    16681 agttccgctc tggacctggt gcatgccgat ctgcaaatgg tcctggagat gatccgggat
    16741 tcgcagccct tggtgcacga cgcgctcgac aatttgcggc acgcaacgcg cgtcattgat
    16801 caggacgtag ttgggacgct cgccgcccag atggacctgg ccaattccgc ttcgctgttg
    16861 gccaaggcgc acagcgccat ggaagacgtc aattcggtca cggccaactt caagagcgtt
    16921 tcggagctcg tgaggaacct gttggtcgtc gacgggcaaa aggtcagcat catcctcgac
    16981 aatcttcagg agatgagcgc ccagctttcc gccaccagcc gcgaattgcg cctgaacccg
    17041 gcgcgcctca tctggggccc cagccgcggc gagcaaaagc gcatcgccgc cttccaggcc
    17101 gcccgcgact tcgcccaggc cgccgaatcg ctcgaccagg ccgccaccga cctgcg
//
LOCUS       DAOSYS010000049        16749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2853_length_16749_cov_4.872888, whole genome shotgun sequence.
ACCESSION   DAOSYS010000049 DAOSYS010000000
VERSION     DAOSYS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_16749_cov_4.872888"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="PLU99_04720"
     CDS             <1..325
                     /locus_tag="PLU99_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16394.1"
                     /translation="WLGRLIECYQQAPQPAAPATRDPPPELTPWSPKRGASGDVEQLR
                     AEAQRLAANWEAQRALIQDLETQREKIWAEAQRLAGLWARQDRYIQQQNRYIAELEAQ
                     LRNGR"
     gene            329..2704
                     /locus_tag="PLU99_04725"
     CDS             329..2704
                     /locus_tag="PLU99_04725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO16395.1"
                     /translation="MRVWIVTSELPHEHAGGIGRYVDNFARTLGAAGHEALVLYRSAT
                     DSDDRPAPGVRTVGFATRDARLAETGAERPDEHPAFPYNLLSFGPALTYQAGAELFRL
                     LESGAERPDVIEVQDYAALGYFLLQRKLTESGPLDDIPIVVHMHSAQFDLMQVNQWPR
                     YRFPEYWLGQMEKFCLRAADAVLSPSEFLRSEVQRSLGRDLEISVIPYPFIADYEREP
                     EPGQTGELLYVGRLEYRKGVMGLVQTCARLWDEGLDFRLTMLGGDTDFAPRCKTIGTL
                     LRERYARWIDCGRLNLAGTVPFDEVATHMRRAAVTLVPSLWENFPNTSIEAMAAGRVA
                     LVSAQGGQAEMVAEDGRNGFIFDWNTPGDLERQLRRVLALSPDERVAIGRRAQERIRS
                     LCDPQRVLPRRLEHYEKVRARHLPRLLFPTTMPADLLRPPPPAEPGPQEEPDLLSVVI
                     PFYNLGAYVDETLASVLNSAYRPLEVIIVDDGSTDPDSISKLAEIEARGLPNVRVVRT
                     PNQGLPGARNVGAEHARGEFLCFVDADDTVEPEYFGRATAVLRRHANVGFVYSWVRWF
                     EGAVSIWITWNAEFPYLLGHNMTVAMAVQRRRWFLEFGRNRSEMAYALEDFEMWISML
                     KNGALGVALPAPLVNYRVRGDSMLRQSQTPQRLYLHDIVTQLHPELYQRFGAELFNLQ
                     NANGPAHAWNQPALDVVMAKESPSLIAELASERALVAELREWARQLEEARDWHNEHRQ
                     MAEKALADAEAQLRSAHATLQELRQSCAAWRRQLPYRLLRRARILSDLQVD"
     gene            2721..5264
                     /locus_tag="PLU99_04730"
     CDS             2721..5264
                     /locus_tag="PLU99_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO16396.1"
                     /translation="MIDPRQPNYASTPAAADRPAFDYAPADPNAPPLVSILTPFFNPG
                     SIFHETACSVFRQSLQQFEWIIVNDATPHEAARALLNEYRGRDPRIRVIDHEVNKGLS
                     AARNTGVAAARSPYVFLLDDDDLLEPTALEKMYWFLETHPEFAVVNGWEVGFGALEYL
                     WPRGFDRGAQILTENCTTGRAMVRCAAHKAIGGHDESIRGGMEDWEYWLRMADHGMWG
                     YTIPEYLDWYRRRENHTARWENLDGAGRQKAFCAWLRSKYPRLYNGRFPRIQPRAHTA
                     FESLRPFKPGINRLAKKSPRLLMIIPRMDLGGAERLCLIFLQTLRAAGWEVTLVATQP
                     GEHAWAPQFARYTPDVFLLDRFLRLPDYPAAIATLIDSRQPDVVMVSGTELGYQLLPF
                     LRAHCPGPTYVDYCHSELAEWKNGGYGRCAAAFQPTLDLNLVTSEHLKNWLVGRGADP
                     SRIKILYANVDTEFWRPCPDTRQAVRAELNLPAERPVVTFVGRLDAEKQPRVLLGTLR
                     RLAKEGVDFTALLVGDGPERAAVEQAIAEHRLAERVRLLGAQPAERIRDLLCASDVFF
                     LPSLWEGIALTVYEAMACGVAVLGADVGGQRELVTPDCGLLLPRSSSEAEAESYARVL
                     ADWLRQPQRLKAMGRAARARVEEHFRLDQMRARLLTLLGEAIEQRRAAPRPVIPAGLA
                     EEWAAEVIELHRMTAVADQYYLQLCGRETAAASPGAGAGGVDDASRLHARLAELDAKL
                     AGQVAQCDAAEPGELTPAQLRHALLQVRDELDLLRERNETLEALYRSEQRGSADLRHR
                     LDDIYRSTGWALLQVLYRWRYALFPSGSRREKAGRWCMHLKRRLSPRAH"
     gene            complement(5305..6303)
                     /locus_tag="PLU99_04735"
     CDS             complement(5305..6303)
                     /locus_tag="PLU99_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO16397.1"
                     /translation="MPLKPRFCAQCGAPVTTRSLQGRQRVVCSACATVFYENPLPVAA
                     TVLLNEQREVLLVKRRRDPHRGQWCLPMGFAELGETIADAARRELQEETGVVGQIVQL
                     LTADSMASDFYGDLLIVTFELTKVGGAEQAGDDAEEVGYFPISHHPPLAFAANEKAVL
                     ACCSAHQEAWAIQDSFAKLRSSESQPLLSDELAALIQEHCEELARLWLADVRSNPTTP
                     SYHRGDAAELLARGTQAVAELSRWLTGQTPNAKLKAFYRDVAGERKADEFALHEVLSA
                     VALLKKQVWNFAAAHVTGGRPIDAYRLLELSRRIAVFFDLAMYQVARGYESEAQGP"
     gene            complement(6294..7079)
                     /locus_tag="PLU99_04740"
     CDS             complement(6294..7079)
                     /locus_tag="PLU99_04740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO16398.1"
                     /translation="MASLSLTAFETPAHSKISAIIRNRSTNPVDVRAAALHGLQLGHA
                     RIILDVGCGFGFMTEAVVPRVRPDAQFVGVDAGAGNEQPYLARLRAAGGQGRFVCRRL
                     HAELSWPDAHFDLVLASYVLYFFPGLLPEVARVLAPDGLLIAITHAAHCGTSLADLID
                     IPDLRARWQALIGRFSGDNGAALLEPWFKIVERVDYPNALVFTSEQCDELLAYLEFKL
                     RLLAPEVLASEALARAARILAAEGRIVLAKDDVVFRCRQPRCR"
     gene            7392..8540
                     /locus_tag="PLU99_04745"
     CDS             7392..8540
                     /locus_tag="PLU99_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO16399.1"
                     /translation="MKDSNRVGARAKSVRNSPPKSGRGIPAPYAERIAALRAECSRRR
                     LDGYLVLNRMDQIWLTGFTGEDGMVLVTPRAVVLLTDGRFAEAASIQAPFAKAVLRKL
                     RTQKANAEIIGRYKLARVGLDPGQISLADFNELKKWARPTRLVAAGGVIGQMRTRKDD
                     GEVASLGKAIDVAQRAFERLRKWISPGVTEQQVAARLEYEMKMLGAQGPSFPTIVACG
                     ANSSLPHYEPGTRPVTENEPILVDWGAQVGWYCSDLTRMIWLGKPRREMVKINAVVRE
                     AHDRAIEAARAGMTAHALDKVARDVISKAGYGKQFAHSLGHGLGLDVHEAPRVGMNST
                     TVLEPGMVVTIEPGVYVPGVGGVRIEDDILITDDGCRVLTSMEIENPK"
     gene            8666..9151
                     /gene="accB"
                     /locus_tag="PLU99_04750"
     CDS             8666..9151
                     /gene="accB"
                     /locus_tag="PLU99_04750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOO16400.1"
                     /translation="MDINQLKNLVQLMVENDLTRVELREGETHILLRRGFPPVVAAAG
                     GMVAQPTVGAVPSAAPSQVLPTSGEEASNSPPADPDEYLIRSPMVGTFYAQHDPESPP
                     FVNVGDEVGPDTVVCLVEAMKVFNEIKAEVSGRITKVLVKNAQAVEFDQPLFAVSLAH
                     E"
     gene            9186..10574
                     /gene="accC"
                     /locus_tag="PLU99_04755"
     CDS             9186..10574
                     /gene="accC"
                     /locus_tag="PLU99_04755"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOO16401.1"
                     /translation="MFKRILVANRGEIALRILRACRDLGIEAIAVYSRADRDAAYLNL
                     AHDAICIGPEQPQLSYLNAPAIISAAEVADVQAIHPGYGFLAENATFAQMCRDCRIEF
                     IGPSPEAMRLLGDKVEARKLAKRAKVPIVPGSIGPVSNEDEAVAVAEKIGFPVMIKAA
                     AGGGGRGMRPAHNEATLRSNLRNARAEAEVAFKDGSLYVEKLIERARHVEVQFLVDQR
                     GNAVHLWERDCSIQRRHQKLIEESPSPHISPRTRSKLCAAAVRLALAARYHSAGTCEF
                     LVDADENLYFMEVNARIQVEHPVTELVTGIDLVQWQIRIAAGEKLAFRQDDVRQDGAA
                     IECRINAESPDDDFRPCPGRITEFIVPGGPGVRVDTHAYCGYAVSPYYDSLIAKLLVH
                     RRSRAEAIASMRRALGEFKIGPVKTTIPLHLALMEHTDFVKGSVDIGFLERTLLGSAR
                     ELKPTADAAALV"
     gene            complement(10687..11127)
                     /locus_tag="PLU99_04760"
     CDS             complement(10687..11127)
                     /locus_tag="PLU99_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HOO16402.1"
                     /translation="MTRLVKIALALIVPGLACAQANETPAQNDNGGLRFQDFDACGVL
                     AEGNGCILFEGGGGTYVLPDADGFDFGDAVRVVGTLDPECITICDEADGCIRGAAVYN
                     PAVYPCGSSLPNFPGDIITNTCSSVGAALPAAALAGLWLTRRRR"
     gene            11557..11874
                     /gene="ndhC"
                     /locus_tag="PLU99_04765"
     CDS             11557..11874
                     /gene="ndhC"
                     /locus_tag="PLU99_04765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOO16403.1"
                     /translation="MLLASYVLGERHRQHATGEPYESGIVSTGSARLRFPVKYYLVAV
                     DFVIFDLEAAFLFAWAVAVRQVGWTGFIGAAIFIGVLLVGLVYLIRGGTLDWATRGGR
                     GYA"
     gene            11871..12530
                     /locus_tag="PLU99_04770"
     CDS             11871..12530
                     /locus_tag="PLU99_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOO16404.1"
                     /translation="MTEEASSTTEPRDQAYLGRTGLPGTGEFEKVAQGNFLLTRLHDL
                     VAWCRRNSVWPFNFGLSCCYVEMATSFTSKYDIARFGAEVIRGSPRQADLIVIAGTVF
                     MKMAPVIQRLYEQMMAPRWVISMGSCANSGGMFDIYSVVQGVDKFLPVDVYVQGCPPR
                     PDTFMEGLLLLTRLVGTEKRPLSWVVGPQTVERPPVPIMRDLKRAQRQRVTELPNPDQ
                     V"
     gene            12541..14310
                     /gene="nuoC"
                     /locus_tag="PLU99_04775"
     CDS             12541..14310
                     /gene="nuoC"
                     /locus_tag="PLU99_04775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HOO16405.1"
                     /translation="MSSAPTTRPAATGVAAELESRFADAILDTQATADGIPTLWITPQ
                     RVRDVLRWLKSQAPQPYRMLYDLTALDERVRKNGHQQLRPDFSVLYHLFSFERNADIR
                     LKVALHGDSPAIQTITDIWPAANWYEREVWDMFGIRFEGHPDLRRILNPPGWVGHPLR
                     KDHPARATDMPPFLLSHAKETAEQERLRFHPEEWGLKRQSEQSDFMFLNLGPHHPGTH
                     GVLRLVLQLDGEEIVDVVPDIGYHHRGQEKMGERQSWHSYIPYTDRIDYLGGVTNNLP
                     YVLAVERLAGITVPPRAQVIRVMLSELFRIISHLVWYGTFSQDLGQMSPVFYMFTDRE
                     RAFSIIEAICGARMHPNWFRIGGVADDLPQGWERLVREFLDYMPPRLREYDKVVLQNR
                     IFKARTQGIGACTRDEGIEWGMTGPMLRACGFEWDFRKKQPYSGYEQFEFDIPVGERG
                     DMYDRALVRVAEMRQSLRIMEQCLRNMPGGPVKSDHPLTTPPQKRYTMHDIETLIDHF
                     LNVSWGPVIPPGECSFRTEGTKGAYSFYLVSDGNTMSYRTRVRTPSFPHLQMVPLMTR
                     GRMIPDLVAILGSVDYVLADVDR"
     gene            14323..14790
                     /gene="nuoE"
                     /locus_tag="PLU99_04780"
     CDS             14323..14790
                     /gene="nuoE"
                     /locus_tag="PLU99_04780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOO16406.1"
                     /translation="MLSDIEKREIEQELAHNHQKRGAAPEALKIVQRHRGWVSDEALR
                     DTAELLEMSPAELDEVATFYNLIFRKPVGRHVILICNSVSCHIMGYPGLREHLRARLG
                     IDFGETTADGKFTLLPMVCLGACDRAPAMMIDEQLYGELTPEKIDEALASYAK"
     gene            14799..16076
                     /gene="nuoF"
                     /locus_tag="PLU99_04785"
     CDS             14799..16076
                     /gene="nuoF"
                     /locus_tag="PLU99_04785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOO16407.1"
                     /translation="MEKPLTKNIRRGEEPLDLRGYERAGGYQALRKAIKEMTPQAVQE
                     TVKAAKLRGRGGAGFPTGVKWSAMPPLEGSRHPRFLAVNADEMEPGTFKDRLLLEGDP
                     HQMVEAVIVSAYAIQADVAYIFLRWAYHLAARRLQAAIREAHAAGYLGRNILGSGYSL
                     ELHVHSSAGRYMCGEETGLLNALEGRRANPRTKPPYPQMVGLWGLPTVVNNVETLCCV
                     PHIVNHGAEWFRKLSLTADGGTKVYGVSGRVKRPGLWELPMGTPLREILEEHAGGMRE
                     GYQFRGVLPGGASTDFLTADHLDLPMDFDAPQKAGSRLGTGTMIVLDDRTCPVGMVQN
                     LEEFFAQESCGWCTPCRDGLPWAARLLRALEEGQGAPGDLEILEQLCWRLGPGHTFCA
                     LAPGAVEPLQSALKYYRADFERHIAERRCPWRA"
     gene            16077..>16749
                     /locus_tag="PLU99_04790"
     CDS             16077..>16749
                     /locus_tag="PLU99_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015699849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO16408.1"
                     /translation="MPRIYVDGRAYDVPEGRNLLHACLSLGLDLPYFCWHPALHSVGA
                     CRQCAVKLFKDESDQRGRIVMACMTPVTEGMRVSIDDPQAREFRAAIIELLMANHPHD
                     CPVCDEGGECHLQDMTVMTGHVHRRFRFAKRTHRNQDLGPFVTHEMNRCIACYRCVRY
                     YCDYAGGRDFGVFGWHDEVYFGRERDGVLESEFSGNLVEVCPTGVFTDKTLARHYTRK
                     WDLETA"
BASE COUNT         3043 a         4965 c         5777 g         2964 t
ORIGIN      
        1 ctggctgggg cggctgatcg agtgttacca acaggcgccg caacccgccg cgcccgcgac
       61 ccgcgatccg cctccggagt tgacgccgtg gtcaccgaaa cgcggcgcca gtggcgacgt
      121 ggaacagctc cgggccgaag cccaacgcct ggcggccaac tgggaagcgc aacgcgcgtt
      181 gatccaggac ctcgagacgc agcgcgaaaa gatctgggcc gaagcgcaac gactggccgg
      241 actgtgggca cgccaagacc gttatatcca gcagcagaac cggtatatag cggagctgga
      301 agcccaatta cgcaacggga ggtgaggaat gcgcgtttgg atcgtcacgt ccgagttgcc
      361 acatgaacac gccggcggca tcgggcgcta cgtggacaac ttcgcccgca cgctgggcgc
      421 ggccgggcac gaggcgctcg ttttgtaccg cagcgcgacc gatagtgacg accggcccgc
      481 acccggggtg cggacggtgg gcttcgcgac ccgtgacgcg cggctggctg aaaccggcgc
      541 ggagcggccg gacgagcatc ccgcctttcc gtataacctg ctgagcttcg gtccggcact
      601 tacctaccag gcgggcgcgg agctgttccg gctgctggaa agcggcgcgg agcgcccgga
      661 cgtgatcgag gtgcaggact acgcggcgct ggggtatttc ctgttgcagc gcaagctgac
      721 cgaatccggc ccgctcgacg acatcccgat cgtggtccac atgcacagcg cccagtttga
      781 cctgatgcag gttaaccagt ggccgcgcta ccgctttccc gagtactggc tggggcaaat
      841 ggagaagttc tgcctacgcg ccgccgacgc cgtgctcagc ccgagcgagt ttttgcgaag
      901 cgaggtgcag cgctcactgg ggcgggacct ggaaatcagc gtcattcctt acccgttcat
      961 cgcggattac gagcgcgaac ctgagccggg ccagacaggg gagttgctat acgtggggcg
     1021 gctcgaatac cgcaagggcg tcatgggtct ggtgcagacg tgcgcccggc tgtgggatga
     1081 gggcctggac ttccgcctga ccatgctggg cggcgatacg gatttcgccc cgcgctgcaa
     1141 aaccatcggc acgctgctgc gcgagcgcta tgcccgatgg atcgactgcg gtcgccttaa
     1201 cctggccggc accgtgccct tcgacgaggt cgcgacgcac atgcggcggg cggccgttac
     1261 gctggtgccg agcctgtggg agaacttccc gaacacatcc atcgaagcga tggccgccgg
     1321 acgcgtggcg ttggtgtcgg cgcagggcgg acaggccgag atggtggcgg aagacgggcg
     1381 caacggcttt atctttgact ggaacacccc cggtgacctg gaacgacagc tccgccgggt
     1441 gctcgccctc agccctgatg agcgggttgc gatcgggcgc cgggcgcagg agcgcattcg
     1501 cagcctgtgc gatccgcagc gggtgctgcc gcgccggctg gagcactacg aaaaagtgcg
     1561 ggcgcgacat ctgccgcgcc tgctgttccc gacgacgatg ccggccgacc tgctgcggcc
     1621 tccgccgccg gccgagccgg gaccgcagga ggaaccggac ctgctgtcgg tggtcatccc
     1681 cttctacaac ctgggggcgt acgtggacga aacgctggcc agcgttctca acagcgctta
     1741 ccgcccgctt gaggtgataa tcgtcgacga tggcagcaca gaccccgaca gcatcagcaa
     1801 gctggccgag attgaagcgc gtggacttcc gaatgtgcgc gtggtgcgta cgccgaatca
     1861 gggcttgccg ggggcgcgta acgtcggcgc cgagcacgcc cgcggagaat tcctgtgttt
     1921 cgtggacgcc gacgatacgg tagagccgga gtattttggc cgcgctacgg cagtacttcg
     1981 ccggcacgcc aacgtcggct tcgtctattc gtgggtgcgt tggttcgagg gggccgtaag
     2041 catctggatt acctggaatg cggaattccc gtacctgctg ggccacaaca tgaccgtggc
     2101 catggcggtc cagcgccgcc gttggtttct ggaattcggc cgcaaccgct ccgagatggc
     2161 ctacgcgctc gaagatttcg agatgtggat cagcatgctc aagaacgggg cgctgggagt
     2221 ggccttgcca gccccgctgg tcaactaccg cgtgcgcggc gactccatgc tacggcaatc
     2281 gcaaacgccg caacggctat acctgcacga tatcgtgacg cagctacatc cggagctcta
     2341 tcagcggttc ggggcggaac tctttaacct gcagaacgcc aacgggccgg cgcacgcctg
     2401 gaatcaacct gccctcgacg tggtcatggc gaaggaatcc ccgagcctca tagctgaatt
     2461 ggccagcgag cgcgccctgg tcgcggaact gcgggagtgg gcccggcagt tggaggaggc
     2521 gcgcgactgg cacaacgagc atcgccagat ggcggagaaa gccttggccg acgccgaagc
     2581 ccaattgcgc agcgcgcacg ccactttgca ggagttgcgc cagtcgtgcg ccgcctggcg
     2641 ccggcaactg ccgtatcgcc tcctgcggcg cgctcgaatc ctcagtgatt tgcaggtgga
     2701 ctgaagcaac cgagcagggc atgatcgacc cgcgccagcc aaattacgcc agcacgcccg
     2761 ccgccgcaga ccggccggcc tttgactacg cccccgcgga cccgaacgcc ccgccgctgg
     2821 tgtccatcct gacgcccttt ttcaaccccg gcagcatctt tcacgaaacg gcctgctccg
     2881 ttttcaggca gagcctgcag cagttcgagt ggattatcgt caatgacgcc acgccgcacg
     2941 aggccgcccg cgccctgctg aatgagtacc gcgggcgcga cccgcgcatc cgcgtcatcg
     3001 accacgaggt caacaaaggc ttgtcagcgg cccgcaatac gggcgttgcg gcggcccgca
     3061 gcccgtacgt gttcctgctc gacgacgacg atctgctcga accgacggcg ctggaaaaaa
     3121 tgtactggtt tctcgagacc catcccgaat tcgcagtggt caacggctgg gaggtgggct
     3181 tcggcgcctt ggagtacctt tggccgcgcg gcttcgatcg cggcgctcaa attctcacgg
     3241 aaaactgcac gaccgggcgc gcgatggtcc gctgtgcggc ccataaggcc atcggcgggc
     3301 acgacgagag catccgcggc gggatggagg attgggagta ttggctgcgc atggcggatc
     3361 acgggatgtg gggctacacc atccccgaat acctcgactg gtaccgccgg cgcgaaaacc
     3421 ataccgcacg ctgggaaaac ctcgacggcg ccggccgcca gaaagccttc tgcgcgtggc
     3481 tgcgaagcaa gtacccgcgc ctgtataacg gccgctttcc gcgaatccag ccccgtgcgc
     3541 atacggcctt cgagagtctg cggccgttca agccgggaat aaaccgcctg gcgaagaaga
     3601 gcccgcgtct gctgatgatc atcccgcgga tggacctggg cggcgcggag cgactgtgcc
     3661 tgatcttcct tcagacgctg cgggccgccg gttgggaagt cacgctggtc gccacacagc
     3721 cgggcgagca cgcttgggcg ccgcagttcg cacgctacac acccgacgtt tttctgctgg
     3781 atcggttcct gcgactgccg gactatccgg ccgccatcgc cacgctgata gactcgcgcc
     3841 agccggacgt ggttatggtc agcggcaccg agctgggtta ccaactgctc ccctttttgc
     3901 gcgctcattg ccccggccca acctatgtcg attattgcca ttctgagctc gcggagtgga
     3961 agaacggcgg atacgggcgg tgcgcggcgg cgtttcagcc gaccctggac ctgaatctcg
     4021 taacttccga gcacctgaag aactggctgg tggggcgcgg cgcggaccca agccggataa
     4081 aaatcctata cgcgaacgtc gacaccgagt tctggcggcc gtgccctgat acgcggcaag
     4141 ccgtgcgggc ggagctgaac ctgcccgccg agcgcccggt ggtgaccttc gtcggccgac
     4201 tggacgccga gaagcagccg cgcgtcctgc tgggcaccct gagacggctg gcgaaagagg
     4261 gcgtggattt caccgcgctg ctggtgggcg acggcccgga gcgcgccgcc gtggagcagg
     4321 cgatcgccga gcatcggctt gcggagcgcg tgcggctgct gggcgctcag ccggccgagc
     4381 gcattcgcga tctgctatgc gcgtcggacg tgttctttct gccgtcgctg tgggagggca
     4441 tcgcgctgac ggtctacgaa gcgatggcct gcggtgtggc cgtgctcggc gcggacgtcg
     4501 gcggccagcg cgagctggta acaccggatt gcggccttct cctgccgcgc agcagctccg
     4561 aggccgaggc cgaaagttac gcgcgcgtgc tagctgattg gctgcggcag ccacagcggc
     4621 tgaaggcgat gggccgcgcc gcccgcgcgc gggtcgaaga gcacttccgg ctggaccaga
     4681 tgcgtgcgcg gctgctgacg ctgctgggag aggcgatcga acagcgccgc gcggcgccgc
     4741 ggccggtgat accggcaggg ctagcagagg aatgggcggc cgaagtcatc gaattgcacc
     4801 gcatgacggc agtggcggac cagtactacc tccagctctg cggtcgcgaa acagccgctg
     4861 catcgcccgg cgccggtgct ggcggggtcg acgatgcaag ccggctgcat gcgcgcctgg
     4921 cggaactgga tgcgaagctg gccggccaag ttgcacagtg cgatgcggcg gaaccgggcg
     4981 aattgacccc ggcgcagctt cgccatgcgt tgctgcaagt gcgtgatgaa ctggacctgc
     5041 tcagggaacg aaacgaaacg ctcgaggcgc tataccggtc tgagcagcgc ggctccgccg
     5101 acctccggca ccggctggac gacatctacc gaagcacggg gtgggcactg ttacaggttc
     5161 tgtaccggtg gcgctacgcc cttttcccat ccggcagccg gcgcgagaaa gccgggcgct
     5221 ggtgcatgca cttgaagcgc cgcctatcgc cccgcgccca ttagcccggt gccgacaaac
     5281 ggtggccccg cccgccacga ggcctcacgg gccttgcgcc tcgctctcgt agccgcgcgc
     5341 cacttggtac atcgccaggt cgaagaatac ggcaattcgc cggctcagct cgagcagccg
     5401 gtaggcgtcg atcggccggc ctccggtgac gtgtgcggcc gcgaagttcc aaacctgctt
     5461 ttttagcagc gcgaccgcgc tgagcacctc gtgcagcgcg aattcgtccg ccttgcgctc
     5521 tccggccacg tcccggtaga aggctttcag cttcgcattc ggcgtctggc cggtcagcca
     5581 gcgactcagt tcggccaccg cctgcgtgcc gcgcgccagc agttcggccg cgtcgccgcg
     5641 gtggtatgag ggcgtggttg gatttgatcg cacatccgcc agccacagcc gggccagttc
     5701 ctcgcagtgc tcctggatta gcgccgccag ctcatccgac agcaacggct ggctttcact
     5761 gctgcgaagc ttcgcaaacg aatcctgtat ggcccacgcc tcctgatgcg ccgaacaaca
     5821 ggccagcacc gccttctcgt tagccgcaaa cgccaacggc ggatggtgcg agatggggaa
     5881 gtaaccgact tcctccgcgt cgtcgccggc ctgctcggcg ccgccgacct tcgtcaactc
     5941 gaacgtaacg atcagcaggt caccataaaa gtcgctggcc atggagtcgg ccgtaagcaa
     6001 ctgcacgatt tgccccacca cacccgtctc ttcctgcaac tcgcgccgcg cggcatccgc
     6061 gatggtttcg cccaattcgg cgaaccccat cggcaggcac cactgcccac ggtgcgggtc
     6121 ccgccgccgt ttgaccagca gaacctcgcg ctgctcattc agcagcaccg tcgccgccac
     6181 cggcagtgga ttctcgtaaa acaccgtcgc gcacgcggaa cagacgacgc gctggcgtcc
     6241 ctgcaaactt cgcgtcgtga cgggcgcacc gcattgggcg caaaaacggg gcttcagcgg
     6301 catcgcggct gcctgcaacg gaagactacg tcatctttgg cgagcacgat ccgtccttcg
     6361 gccgcaagga tacgcgccgc gcgggcgagc gcctccgacg ccagcacctc cggcgccagc
     6421 agccgcagct tgaactcgag ataggccagc aattcgtcgc actgctcgct cgtgaagacc
     6481 agggcgttgg gatagtctac gcgttcgacg attttgaacc aaggttccag cagcgccgca
     6541 ccattgtcgc cggaaaaacg accgatcaag gcctgccagc gggcgcgcaa gtcggggata
     6601 tcgatcaggt ctgccaggct agtgccgcaa tgggcggcgt gggtaattgc gatcagcagc
     6661 ccatccggag ccagcacgcg cgcgacttcc ggcagtagcc ccgggaaaaa gtacaggacg
     6721 tatgaggcca gcaccaagtc aaagtgcgcg tccggccagg acaactctgc gtgcagccga
     6781 cggcagacga agcgcccctg gccgccggcc gcgcgcaggc gtgctaagta cggttgctcg
     6841 ttgccggcgc cggcgtccac gccaacgaac tgggcgtcgg gccgaacgcg tggcacgacc
     6901 gcttcggtca tgaatccaaa gccgcaacca acgtcgagga ttatccgggc gtggccaagc
     6961 tggaggccgt gcaacgccgc ggcgcgcacg tcaaccggat ttgtggacct gttcctgata
     7021 atggcggaga tcttagagtg cgccggcgtc tcgaaagctg tcagagagag gctcgccata
     7081 ccactaatta ttatcacgcg ttgcctgcga cctccaagca aaatctgcga aggccggcga
     7141 ttagcgatgc ccgcccggcg gcggcgtttg gcgacttttg ggtcgggttc caagcagcgc
     7201 gaacctgact cccgactctg ttttctgatt actccatcgg cgttatcgaa tcgatgttcg
     7261 cggcctgcga tccgctcgga ggcgcactac ccactggtca ggcccgatgc gcggggttaa
     7321 attgcctctg gaaccggggc gcggcccggc ccgcaaggca ttgccgacac cggttttcgg
     7381 agcgctgaga aatgaaagac agcaacagag ttggcgcgcg ggcgaaatcc gtccgtaatt
     7441 cgccgcccaa gagcggacgc ggaattccgg ccccatacgc ggaacgcatc gcggctctgc
     7501 gggcggagtg cagccggcgg cgattggatg gatatcttgt tctaaaccgg atggatcaga
     7561 tctggctcac gggcttcacc ggcgaggacg ggatggtgct cgtgaccccg cgagccgttg
     7621 tgttgctgac cgatggtcgg tttgcagagg cggcttcgat ccaagcgccc ttcgccaagg
     7681 cggttctgcg gaagctgcgg acgcagaagg cgaatgccga gatcattggc cggtacaagc
     7741 tggcgcgggt ggggcttgat ccgggtcaga taagtctggc ggattttaac gagctgaaga
     7801 agtgggcgag gccgacccgc ctggtagcgg cgggcggggt gatcgggcaa atgcggacgc
     7861 gcaaggacga tggcgaggtg gcgtcgctcg gcaaggcgat cgacgtcgcc cagcgcgcgt
     7921 tcgagcgcct gcgtaagtgg atttcgcccg gcgtgaccga gcagcaagtg gccgcccggc
     7981 tggaatacga gatgaagatg ctgggggcgc aggggccgtc gttcccgacg atcgtcgcat
     8041 gcggcgcgaa cagctcgctg ccgcactatg agccggggac gcgtccggtg accgaaaatg
     8101 agccgatcct ggtcgattgg ggtgcgcaag tcggctggta ttgcagcgat ctgacgcgca
     8161 tgatctggct gggcaagcca cggcgggaaa tggtgaagat caacgcggtc gttcgcgagg
     8221 cgcacgatcg ggcgatcgaa gcagctaggg cgggcatgac ggcgcacgcc ctggacaagg
     8281 tggcgcgcga cgtgatcagc aaggccggct acggcaaaca gttcgcacac tcgcttggcc
     8341 acggcctggg gctggacgtg cacgaggcgc cgcgggtggg gatgaactcc accacggttt
     8401 tggagccggg gatggtggtg acgattgaac cgggcgttta tgtgcccggc gtcggaggcg
     8461 tgcggatcga ggacgacatt ctgatcacgg atgatggatg ccgggtatta acgtcgatgg
     8521 agattgagaa tccgaaatag aatgggatcg aaggtccagg ggtcactgcg cgggcgctgc
     8581 ccgcaaaccc gattgaaatc aacgccctgc tcaagagcag tggttgccgg ccgctgctta
     8641 gcggcagtgg cacagtagag agccgatgga cataaaccaa ttgaagaatt tggtgcagct
     8701 catggtcgag aacgacttga cccgcgtgga gctgcgcgaa ggcgaaacgc atattctgct
     8761 gcggcgcggg ttcccgccag tggtggcggc ggccggtgga atggtggcgc agccaacggt
     8821 cggagccgtg cctagcgcag cgccgtcgca ggtgctgccg acaagcggcg aggaggcaag
     8881 taattcaccg ccggccgatc cggacgaata tctgatccgt tcgccgatgg tgggtacgtt
     8941 ttacgcgcaa cacgatccgg agtcgccgcc attcgtcaac gtgggcgacg aggttggacc
     9001 ggatacggtg gtttgcctgg tcgaggcgat gaaggtcttc aacgagatca aggccgaagt
     9061 gagcgggcga atcacgaagg tgctggtgaa gaacgcgcag gcggtcgagt ttgaccaacc
     9121 cttgttcgcg gtctcgctgg cgcacgagta ggccgaactg gcgaaacccc tctggcgaga
     9181 tcgtgatgtt caagcgcatt ctggtagcca accgcggcga gatcgcgctg cggattctgc
     9241 gggcctgccg cgatctaggt atcgaggcca ttgccgttta cagccgggcg gatcgcgatg
     9301 cggcgtattt gaacctggcg catgacgcga tctgcattgg tccggagcag ccgcaacttt
     9361 cgtacttgaa cgcgccggcc atcatctcgg ccgcggaagt ggccgacgtt caggcgattc
     9421 acccggggta cggctttctg gccgaaaacg cgaccttcgc gcagatgtgc cgcgactgcc
     9481 ggatcgagtt catcgggccg agccccgagg ccatgcgtct gctgggcgac aaggtcgaag
     9541 cgcgcaagct ggccaagcgc gccaaggtgc ccatcgtgcc gggcagcatc gggccggtgt
     9601 ccaatgaaga cgaggcggtc gcggtggccg agaagatcgg ctttccggta atgatcaagg
     9661 cggccgcggg cgggggtggg cgtgggatgc ggccggcgca caacgaggcg acgctgcggt
     9721 cgaacctgcg caacgcccgg gccgaggcgg aagtggcttt caaggacggc agtctgtacg
     9781 tcgagaagct gatcgagcgg gcgcggcacg tagaggttca gttcctggtt gatcagcggg
     9841 gcaacgcggt gcacctgtgg gaacgcgatt gctcgattca acggcggcat cagaaattga
     9901 ttgaggaatc gccatcgccg catatcagtc cgcgcacacg ctcgaagctg tgtgcggcgg
     9961 cggtgcggtt ggcgctggcg gcgcggtatc actcagcggg gacctgcgaa tttctggtgg
    10021 atgccgacga aaatctttac ttcatggaag tgaacgcgcg catacaggtc gagcacccgg
    10081 tgactgagct ggtgaccggg atagacctgg tgcagtggca aattcgcatt gcggcgggcg
    10141 agaagctggc gtttcgtcag gacgatgtgc gccaggacgg cgcggcgatt gagtgccgca
    10201 tcaacgccga gtcgccggac gacgacttcc ggccgtgtcc ggggcggatc acggagttta
    10261 tcgtgccggg cgggccgggc gtgcgtgttg atactcatgc gtactgcggt tacgcggtca
    10321 gcccgtatta tgactcactg atcgccaaat tgctggtaca ccggcggtca cgtgccgagg
    10381 cgatcgcgtc gatgcggcga gcgttggggg aattcaagat cgggcctgtg aagaccacga
    10441 ttccgctgca tttggcgctg atggagcaca ctgattttgt caaaggcagc gttgatatcg
    10501 ggttcttgga gcggacgctg cttggttcgg cgcgcgaact taagccgacg gcggatgcgg
    10561 cggccctggt gtagtgcgcg acgaaaaatg cggggctccc agtaatagtg ataaggtgat
    10621 agggtgataa agggtccgcg gagcgtcctt tatcacccta tcactttatc accttaccac
    10681 gtcatctcag cggcggcggc gggttagcca taggccggcc agtgcggcgg cgggcagggc
    10741 cgctccgacg gatgagcacg tattggtgat gatgtctccg gggaaattcg gcaagctgga
    10801 gccgcagggg tagaccgcgg ggttatagac cgccgcgccg cggatgcagc cgtcggcctc
    10861 gtcgcagatc gtgatgcatt ccgggtccag agtgccgaca actcgaactg cgtcgccaaa
    10921 atcgaaaccg tcggcatcgg gtaggacata cgttccgccg ccgccctcga aaaggatgca
    10981 gccgttgcct tctgccagta cgccgcaggc atcaaagtcc tgaaagcgca acccgccatt
    11041 gtcgttctgg gcgggcgttt cgttagcctg ggcgcaggct aagccgggga cgatcagggc
    11101 gagagcaatt tttacgagtc gcgtcatcga aaatctccag gcttggcatt gtacgctaag
    11161 ccagcgcgcg gcggcagtgc gccgcgaggc gcgcgtcgtc ggagggattg cctgacggaa
    11221 ggctgcgcac aagcactttc gaggcgctaa agcctttgcc aggctcgccc gccctccaga
    11281 cgatgaccac gggtagcgcc gttcgccaag atcaaccttt caggatcgta atctgcccag
    11341 cccctgtaat ctgatcgcga gactgctaga ataggctgta atgctcacgg gccggccctc
    11401 ttaaatcggt tggttcgtgt cggctgttcg ggcctgaaga aactgcgtat tccggtaacg
    11461 gcgcgatagg agcggcgagt ggaggctggg gacacggttc agccactatg gccaatcgta
    11521 atctacgcgg gcgcttgcgt ggcgctggcg gcgagcatgc tgctggcctc gtacgtcctc
    11581 ggcgagcggc accggcaaca tgcgacaggt gaaccgtacg aatcgggtat agtctcgacc
    11641 ggctcggcgc ggctgcggtt tccagttaag tactacctgg tggcggtaga cttcgtgatt
    11701 ttcgatttgg aggcggcgtt tctgtttgcc tgggcggtgg cagtgcgcca ggttgggtgg
    11761 accgggttta tcggagcggc catctttatc ggcgtgctgc tggtcgggct ggtgtacctg
    11821 atacgtggag gtacgctgga ctgggcgacg cgcggaggac gaggttacgc atgactgaag
    11881 aggcgtcgag cacgaccgag ccgcgcgatc aggcctacct gggtcggact ggattgcccg
    11941 gcaccggcga gtttgagaaa gtggcccaag gcaactttct gctgacgcga ctgcacgatc
    12001 tggttgcctg gtgccgacgg aattccgtct ggccgttcaa ttttgggctt tcctgctgct
    12061 atgtggagat ggcgaccagc ttcaccagta agtacgacat tgcgcggttc ggggcggagg
    12121 tgatccgcgg ctcgccgcgc caggctgacc tgatcgtgat tgccggcacg gtattcatga
    12181 agatggcccc ggtcattcag cgcctttatg agcagatgat ggcgccgcgc tgggtgatct
    12241 cgatggggtc ctgcgccaat tccggcggaa tgtttgacat ttacagcgtg gtacaggggg
    12301 tggacaagtt tctgccggtg gacgtgtatg tgcagggctg tccgccacgg ccggatacgt
    12361 ttatggaggg gctattgctg ctgacgagac tggtgggtac ggagaagcgg ccgctcagtt
    12421 gggtggtagg gccgcagact gtcgagcggc cgccggtgcc gatcatgcgc gacctgaaac
    12481 gtgcccagcg gcaacgagtg acggaactgc ctaacccgga tcaggtgtga gtcaagcacg
    12541 atgagcagtg cgccgacaac tcgaccggcc gcaactggtg tggccgcgga actggaaagc
    12601 cggttcgcgg acgccattct ggacacgcag gccacggcgg acgggatacc cactttatgg
    12661 attacgccgc agcgggtgcg cgacgtgctg cgctggttga aatcacaggc gccgcagcca
    12721 taccggatgc tctacgacct gacggcgctc gacgagcgcg tccgcaagaa cgggcatcag
    12781 cagttgcggc cggacttcag cgtgttgtac cacctgttct catttgagcg gaacgcggac
    12841 atacgcctga aggtggcgct gcacggcgac tcgcctgcaa tacagacaat caccgacatt
    12901 tggccggcgg cgaactggta cgaacgcgaa gtgtgggaca tgttcgggat ccggttcgag
    12961 gggcatccgg atctgcggcg tattctgaat ccgccgggct gggtgggcca tccgctgcgc
    13021 aaggatcatc cggcccgggc caccgacatg cctccatttc tgctgtcgca cgcgaaggag
    13081 acggccgaac aggagcgctt gcggtttcac ccggaggagt gggggctgaa gcggcagagc
    13141 gagcagagcg acttcatgtt tctgaacctg ggtccgcatc acccgggaac gcatggcgta
    13201 ttgcgactgg tgttgcagct cgatggtgag gagatcgtcg acgttgttcc ggacatcggc
    13261 tatcaccacc gcgggcagga gaagatgggg gagcggcaga gctggcacag ttatattccc
    13321 tatactgatc gcatcgacta tctcgggggc gtgacgaaca atttgccgta tgtgctggcg
    13381 gtcgagcggc tggccgggat cacggtgccg ccgcgggccc aggtcattcg ggtcatgctt
    13441 tcggagctgt tccgcattat cagtcacctg gtgtggtatg gcacgttctc gcaggacctc
    13501 gggcagatgt cgccggtgtt ctatatgttc accgatcgcg agcgggcgtt ctccataatt
    13561 gaagcgatct gcggggcgcg catgcacccc aactggttcc gcattggcgg agtggcggac
    13621 gacttgccgc aaggctggga gcggttggtg cgggaatttc tggattacat gccgccgcgg
    13681 ctgcgcgagt acgacaaagt cgtcctgcag aaccggattt tcaaggcccg cacccaaggc
    13741 atcggcgcct gcacgcgtga cgagggcatt gaatggggga tgaccgggcc gatgctacgg
    13801 gcgtgcggct ttgagtggga ttttcgtaag aagcagccgt attcgggata tgagcaattt
    13861 gagtttgata ttccggtggg cgagcgcggc gacatgtatg accgggctct ggtgcgcgtg
    13921 gcggagatgc ggcagagttt gcggatcatg gagcagtgtc tgcgcaatat gccgggcggg
    13981 ccggtcaagt cggaccatcc gttgaccacg ccgccgcaaa agcgctacac catgcatgac
    14041 attgagacgc tcatagacca ttttttgaat gtgagttggg ggcccgtgat tccaccgggc
    14101 gaatgctcat tccgcacgga agggaccaag ggcgcctaca gcttttatct ggtgagcgat
    14161 ggcaatacga tgagctaccg tacgcgcgtc cggacaccct ctttcccgca tttgcagatg
    14221 gtgccgctga tgacgcgcgg ccgcatgata ccggacctag ttgctatctt gggaagtgtt
    14281 gactatgtgc tcgcggacgt ggatcgctag aggccggcgc cgatgctgag tgatatcgag
    14341 aagcgcgaga ttgagcagga gctggcgcat aatcaccaga agcgcggggc tgcgccggaa
    14401 gctctgaaga tcgtgcaacg gcatcgcggc tgggtctcgg acgaggcgct gcgggatacg
    14461 gccgagctgc tggagatgag tccggccgag ctggacgaag tggcgacgtt ctacaatctc
    14521 atattccgca agccggtcgg gcggcacgtg atattgatct gcaatagcgt gagttgccac
    14581 atcatgggat accccggact gcgcgagcac ttgcgggcgc ggctcggtat agactttggc
    14641 gagaccaccg cggatggcaa gtttacgctg ctgcctatgg tgtgcctggg cgcctgcgac
    14701 cgcgccccgg ccatgatgat tgacgagcaa ctatacggcg agttgacgcc ggagaagatt
    14761 gacgaggctt tggcaagcta cgcgaagtag agagagccgt ggagaagccg cttacaaaga
    14821 acatccgtcg tggtgaggag ccgctcgacc tgcgcggcta tgagcgggcc ggcggttatc
    14881 aggcgctgcg caaggcgatc aaagagatga cgccgcaggc ggtgcaggag acggtgaagg
    14941 ccgccaagct tcgcgggcgc ggcggagcgg gctttccgac gggtgtgaag tggagcgcta
    15001 tgccgccgct cgaagggtcg cgtcatccgc gctttctggc agtcaacgcc gacgagatgg
    15061 agccggggac gttcaaggac cggctgttgc tggagggcga tccgcaccag atggtggagg
    15121 cggtgatcgt cagcgcctac gcgatccagg cggatgtagc gtacattttc ctgcgctggg
    15181 cctatcacct ggcggcgcgg cgcttgcagg ccgcaatccg cgaagcgcat gcggcaggtt
    15241 atttgggccg gaacatcctg ggctcgggct acagtctgga attgcacgtc catagtagcg
    15301 ccggacgcta tatgtgcggc gaggaaaccg ggttgctgaa tgcgctggag ggaagacggg
    15361 cgaacccccg caccaagccg ccctatccgc agatggtggg gctgtggggc ctgccgacgg
    15421 tagtcaacaa tgtggagacg ctgtgctgtg ttccacatat agtgaatcat ggcgcggaat
    15481 ggtttcgcaa gttgagtctg actgcggatg gtgggacgaa ggtgtacggc gtcagcgggc
    15541 gcgtcaagcg gcctggcttg tgggaattgc caatgggcac gccgttgcgg gagatactgg
    15601 aagagcacgc gggcggcatg cgcgagggct accagtttcg cggcgtgttg ccgggcggag
    15661 cgtcgaccga ttttctgacg gcggatcatc tggatttgcc gatggatttt gacgcgccgc
    15721 agaaggccgg cagccggctg ggtaccggaa cgatgatcgt gctcgacgac cgcacctgcc
    15781 cggtcggaat ggtgcagaat ttggaggagt tcttcgccca ggagtcgtgc ggctggtgta
    15841 cgccctgccg cgacgggttg ccgtgggcgg cgcggctgct gcgggccctt gaggagggcc
    15901 agggcgcgcc cggtgatctg gaaattctgg aacagctatg ttggcggctg gggccgggcc
    15961 atacgttctg cgctttggcg cccggggcgg tcgagccgct gcagagtgcc ttgaagtact
    16021 atcgcgccga tttcgagcgg catattgccg agcggcgctg tccctggaga gcgtgaatgc
    16081 cgcgaattta cgtggatggc cgggcctacg atgtgccgga ggggcgcaac ctgctgcacg
    16141 catgcctctc gctgggtctg gacctgccgt atttttgctg gcatccggcg ctgcattcgg
    16201 tgggggcgtg ccggcaatgc gcggtcaagc tattcaagga cgagagcgac cagcgcggca
    16261 ggatcgtgat ggcgtgcatg acgccggtaa cggagggcat gcgcgtctcc attgacgatc
    16321 ctcaggcgcg cgagttccgg gccgcgatca tcgaactgct catggcgaat cacccgcatg
    16381 attgcccggt gtgtgacgag ggcggcgagt gccatctgca ggacatgacg gtcatgaccg
    16441 ggcacgtaca ccggcggttc cggttcgcca agcgcacgca ccgcaaccag gatttggggc
    16501 catttgtcac gcacgaaatg aaccgctgta ttgcgtgcta taggtgcgtg cgttactact
    16561 gcgattacgc gggcgggcgg gatttcggcg tatttggctg gcacgacgaa gtgtacttcg
    16621 ggcgcgagcg cgacggcgtg ctcgaaagcg aattcagcgg caacctggtc gaggtgtgcc
    16681 cgacgggtgt gttcacggac aagacgctgg cccggcacta cacgcgcaag tgggacctcg
    16741 aaacggcgc
//
LOCUS       DAOSYS010000050        16623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2886_length_16623_cov_5.146789, whole genome shotgun sequence.
ACCESSION   DAOSYS010000050 DAOSYS010000000
VERSION     DAOSYS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16623
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2886_length_16623_cov_5.146789"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..345
                     /locus_tag="PLU99_04795"
     CDS             166..345
                     /locus_tag="PLU99_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16409.1"
                     /translation="MPKLAPPLLPPPVNVLFVEGCVHFTPREAFEQALAGTYSRLGRE
                     AEMPGELGGGGLPGE"
     gene            complement(370..1296)
                     /locus_tag="PLU99_04800"
     CDS             complement(370..1296)
                     /locus_tag="PLU99_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOO16410.1"
                     /translation="MARVIQIVVPSHTTTPLLNALQQSERTLSIRLQPGVSLEPPGDL
                     VTIEVRNRELMPTLRLLDEQGVGRGPGTVLSTAQPLCIISPTAAAELQNDTSECSFEE
                     IETALVRDTSSDVLLVMAISGFIAVSGLATGALHIVVAGMLIAPGFEPLVRIARGVVA
                     RSAAWRAGLRCTPLPYAALFIGAAIAALVLRFHGKPLLSGSGGYLPTPPLVAYWTNLT
                     LFSVLEVIVAATAGAFLVATNRSILTAGVMIALGLIPAAALVPAALVESQWDLAGRAL
                     LRWAVDAALVVVVSFVVFGWKQLRVHRRQPAY"
     gene            1614..2411
                     /locus_tag="PLU99_04805"
     CDS             1614..2411
                     /locus_tag="PLU99_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16411.1"
                     /translation="MLLAGCSDPVTAPIAEARPLPAPANPARIAQTLPLSVPADVQFI
                     ILHVQTPRAARDQLNRVWDLVREDSVDSETAVRLYSNGVRMGVGQIARWDAIKDIFNA
                     TEKSRVYELPPMHNPPGVPLGLHLDATPRDQTIFFLDDDGILTGDTWPASQKVLRLTY
                     EPDPLQRDSVRLMVVPEIRQELPPALTYTTTEGWATGRRREGRAYGSAAFEIPLSTEE
                     FVMVGPGEKADVFGMVGGAFLTGLVNDERYDTFVFIRAEISHGRPTR"
     gene            2392..3519
                     /locus_tag="PLU99_04810"
     CDS             2392..3519
                     /locus_tag="PLU99_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16412.1"
                     /translation="MAGPPDSRAALVGHLDAGFLHGLFESVGLAIVACEPDGRVIACN
                     SAAADLFGRVQSSTPGIYVAELFPERDRPRLEQLLVDTTTTLEPREYQTQLGRDEAGP
                     LDFAVLATPVLEHDGSLRGVSLWMRDITKRRRLQRQLKRNERLTYLGKLAGAVAHHYN
                     NLLCSIATSLEYAMNMNTLTAMRRAAQRTAEAVGQAADITRQLLAFAQADHRASDLAD
                     LSEIVAEILHGNEQRFKNQRVELRFDYQPLPQVLVDREQMIIVLNHVLNNALEAMPDG
                     GVLRVSLASLGPEIVCLSIADTGPGISPEHMEHLFEPFFTTKGVLGGGKSGNAGLGLA
                     VVHGLVHDMHGAVTAANVPGGGARLDIVLPVERPEDRPPPR"
     gene            complement(3671..3988)
                     /locus_tag="PLU99_04815"
     CDS             complement(3671..3988)
                     /locus_tag="PLU99_04815"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOO16413.1"
                     /translation="MIFGEESSTPQNQPQPQQIRLDLSGMETVYANAFALAGSPDEVV
                     LYVGANLPLPNMKEPVVRVSHRVTLIPANAKRLLIALQQTIKAHEERFGPIELPPPRR
                     PAQ"
     gene            complement(4137..5192)
                     /gene="tsaD"
                     /locus_tag="PLU99_04820"
     CDS             complement(4137..5192)
                     /gene="tsaD"
                     /locus_tag="PLU99_04820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOO16414.1"
                     /translation="MQKADALILGIETSCDETSAAVVAGGRQVLSSLVSSQVALHAKY
                     GGIVPELASRAHVEQVRPIVEGALAEAGVRYSDLDAIAVTVGPGLIGSLLIGVTAAKT
                     LSLAQGLPLVAVDHIEAHATSAALTADTSPWPAVALVVSGGHTSLYLVRDFLRIELLG
                     QTVDDAAGEAFDKVAAILELGYPGGPVIDALAREGDPAAIDFPRPWIHKPHFDFSFSG
                     LKTAVLYHTYGGPHKYGSVAHLSRRQLADIAASFQAALVETLVAKTVAAAEHAGVSTV
                     VVGGGVAANSALREALKQACTERGLTLHLTPLAYCTDNAAMVAALGYRHWLAGETADL
                     GLEPRAGLQRPPRVTTA"
     gene            complement(5258..7456)
                     /gene="feoB"
                     /locus_tag="PLU99_04825"
     CDS             complement(5258..7456)
                     /gene="feoB"
                     /locus_tag="PLU99_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOO16415.1"
                     /translation="MFDPCTGCGERATGVCCCAATTACKTATIALVGNPNTGKTVLFN
                     ALTGFRRKVANYPGVTVDIGEAPIRGSRHDLELLDLPGTYSLAAASPDEMVVCNVLSG
                     RVSGRSRPDVILAVVDASNLRRNLYLLSQLLDFGLPVVVALNMLDVARARGLEIDIAR
                     LAERLGVRVIPVIATDKRTLPPFIAALDAALAELRPPRPAPLPSVIEQSAAQLAASNG
                     QLSRCESLRVLLDNEGYAEREFLAHGGLAAELAAARRLLETAQIDGTAAEIRARYAWI
                     DNVLEGVVARSQHGRSTWSSRLDRVLTHKLGGFALLLIVLCLVFQSIFYAARPLMGAI
                     EALFELVGAQATQLLPEGVFRSLLVDGVIGGVGSVLVFLPQVMILFALIALLEDCGYM
                     ARAAFMVDRLMRGMGLSGLSFIPLLSSFACAVPAIMSTRTIADRRERCVTMLVIPFMS
                     CSARLPVYLVMVGALVPDINYFGGLIGLPALVMLAMYLVGIVVAIPTAWLLRKTAFAG
                     PTSGFLLELPSYKLPRPGTVLQRAYSGGRSFLVRAGSIILVLNLVVWALAYFPHDAAA
                     RSVVEQQAQAADWGPERLKAELDAQQLRDSYLGRLGQGIEPVLRPLGWDWRIGVSVLA
                     SFPAREMVVATLGTLFNLGESSGGGAESLHQALQSATWIGTDQRLFTLPVALSIMVFF
                     ALCAQCGSTLIMIGREMRSIAWPAASFFGMTILAYFAAWGTYAAAQAAGL"
     gene            complement(7522..7746)
                     /locus_tag="PLU99_04830"
     CDS             complement(7522..7746)
                     /locus_tag="PLU99_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HOO16416.1"
                     /translation="MGLVHAPREKALRVTCVRNSGAFGRRLMEMGLIEGAVLAVLRRA
                     PLGDPLHIRLGDYELSLRSREAELIDVSPL"
     gene            8132..8734
                     /locus_tag="PLU99_04835"
     CDS             8132..8734
                     /locus_tag="PLU99_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO16417.1"
                     /translation="MLKSRVFLYLAFLFLVCPLATAEIIDWTCDDDGDGAIVMESALF
                     GPSAEGYSLELICTQNWYPGHLQGDFITDTPEDPTVSLVQIITNDTDFVWTDYHITIG
                     MPQPFSIVGAFGPTDWTWNVTPATDGLALPGHPSPGTGWVGTIDYFAGSPIPIGGSGT
                     FGFVVSFEGTVSFCTEQIPTPEPTSVLLLALGALTVLRRR"
     gene            complement(8999..9073)
                     /locus_tag="PLU99_04840"
     tRNA            complement(8999..9073)
                     /locus_tag="PLU99_04840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9036..9038),aa:Pro,seq:tgg)
     gene            complement(9477..>10686)
                     /gene="hutI"
                     /locus_tag="PLU99_04845"
     CDS             complement(9477..>10686)
                     /gene="hutI"
                     /locus_tag="PLU99_04845"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOO16418.1"
                     /translation="AAMREWPLIEDAALLVRDGRIAWFGPQREAPATHEGERVSDARR
                     LSAAGGCVIPGLIDCHTHIPFAGNRSGEFVRRLAGETYLSIMQSGGGIRVTTAAVRQA
                     SRQQLVDENLPRLRRMLSYGVTTVECKSGYGLSPEHEIKQLAAVRDLAALQPIELVPT
                     YLGAHALPAEFEGRSDEYIELMSSPELLARIAREKLARFCDVFCDRGAFTVEQARTML
                     SRAAAAGLRPKLHADELAQIGASGLAGELQAASADHLERIDDAGITALKSGGAVAVVL
                     PGTSFFLGIEHCPARRLIEAGLPVAIATDFNPGSCMIESLPLVMNIAACQLRLRPTEI
                     VAACTANAAVAVGAAERLGAIAAGYEADLVVLDAPTLDEWFYAVGRPQVRQVLKRGRA
                     VYSATGEEAR"
     assembly_gap    10687..10786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10787..11427)
                     /locus_tag="PLU99_04850"
     CDS             complement(<10787..11427)
                     /locus_tag="PLU99_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOO16419.1"
                     /translation="MKTIGLIGGMSWQSTLAYYRIINERVAKRLGGLHSAKCVLYSVD
                     FAEIEELQSAGDWATAGRAMAEAAQAVERAGADFVVLCTNTMHKVAGAIEAAVRIPLL
                     HIVDATAERIRVAGLRRVGLLGTRYTMEDDFYRGRLESKFGLEVVTPSAPQRQRVHDI
                     IFDDLCRGDTTGAAELQNIVGTLVQNGARGVILGCTELGLAIKPESSPVPLFDT"
     gene            11511..11912
                     /gene="metG"
                     /locus_tag="PLU99_04855"
     CDS             11511..11912
                     /gene="metG"
                     /locus_tag="PLU99_04855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOO16420.1"
                     /translation="MRSFSTGSERRDAARAHRKKAMPEEIIQYDDFLKLDLRVGTITA
                     AEAHPNADKLLVLQVDLGPEQRQLVAGIRAHYEPAQLVGKQIIVVANLAPRMMRGLES
                     KGMLLAASTPDKSQVVLLTTEKPVPPGSTVS"
     gene            complement(12044..13417)
                     /locus_tag="PLU99_04860"
     CDS             complement(12044..13417)
                     /locus_tag="PLU99_04860"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOO16421.1"
                     /translation="MPHKRLGIVVGGGPAPGINAVIGAAVIEAINRGFEVIGFYDGFK
                     WLCSDNFDPPTHSVRLDIKRVARIHFDGGSILRISRTNLLDNESLKTSTVVKPDPYKV
                     GLVVSRLRELGINCLLTIGGDDTALSSRFIAEAAGDRVRTVHVPKTIDNDVPLPQNQA
                     TFGFATALNYGTQLIKNLMQDSQTTGRWYFVTAMGRSAGWLAMSMGISAGATVTLIPE
                     EFTERSSVQRIADVLEGAIIKRRVMGRPDGVALIAEGLAYRLGDREELERLLGRSVPV
                     DAAGHPRLAEVPLAEMLRQEVQARFRARGENMPLVLHTIGYELRSADPTPHDMAYCRS
                     LGYHSIRLFEDGTRRASGCLVALVNENLVALDLHDLVDPATNRIRTRLVDTQSDKYKA
                     ARAYQIRLERSDLEDPIMLARLAVEAHMSPAEFKKRFMRAATPICELGPEEDAAQPEP
                     AAATSAT"
     gene            13667..13864
                     /locus_tag="PLU99_04865"
     CDS             13667..13864
                     /locus_tag="PLU99_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOO16422.1"
                     /translation="MPKQKVHKGLRKRVRVTPRGKVVRKQAFRGHLMSPKSGARRQAL
                     RRIVEVKGKLAQNLKRALCAE"
     gene            13887..14252
                     /gene="rplT"
                     /locus_tag="PLU99_04870"
     CDS             13887..14252
                     /gene="rplT"
                     /locus_tag="PLU99_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOO16423.1"
                     /translation="MPRVLPGHATRRRHKKVLRAASGFRASRSTLYRSAKPTLIRAGV
                     YATRDRRARKREFRALWITRVSAACDARGISYSKFMNGLKNANVILNRKMLSEVAIAD
                     PAAFDKLTELAKSKAKATA"
     gene            14614..16125
                     /locus_tag="PLU99_04875"
     CDS             14614..16125
                     /locus_tag="PLU99_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO16424.1"
                     /translation="MPLSPLEFQRVSFVYETASAPVLSGVTASFGRGWTGIVGPNGCG
                     KSTLLRLACGELEPTSGGIHRPGPVAYCPQRTDHPPPELPEFLSTDEPRACVLRGRLG
                     LQADWLTRWNTLSHGEQKRAQLAVVLARQPAVLALDEPTNHIDSQTRDLLASVLDDFS
                     GIGLLVSHDRELLDRLCRRCLLMSVGQVVLRSGNYSAARAQQQAEEASARAQRERLQH
                     ELRRIEREVSNRRREAARADKLRSKRHLDKHDHDGKGAINLARNSGKDGVAGRRMRQL
                     EGRAAQAAARVDQVQVRAISKMGVALGGERARRPVLLRLDAGRLALGSDRWLVHPELV
                     ILADARIGLTGKNGSGKSTIVRHIVDRLDLPAGRVVYLPQELDAERGAIAATELRKLP
                     DQVLGETLSHISRLGSDPRALLETAQPSPGETRKILLAMGMALQPHLIIMDEPTNHLD
                     LPSIECLEAALADCPAALLLVSHDRRFLKRLTRIRWHIVRDERDSRVEGLHVH"
     gene            16170..>16623
                     /locus_tag="PLU99_04880"
     CDS             16170..>16623
                     /locus_tag="PLU99_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOO16425.1"
                     /translation="MAAEPPSGNFPPKPQSRPSPSSAPHALSAEDVLRDYASDAHSGL
                     SAAQVQELRNRFGPNELARAVGVPWWRRFAAQFADLIIWILIAAALISGVAGEWLDAV
                     VILAIVVLNAVLGFVQEERAERALAALAEMSAPQAKVIRGGRLQTLPAA"
BASE COUNT         3052 a         5348 c         5268 g         2855 t
ORIGIN      
        1 gccaggaccg cgtgcaactg gaactggcgg caaaactgct cgagagcaca tctgagcggc
       61 aggaaggccc tccgcgcgtc atatcggtca tattgttaga tttgctgctg ccgatgtcga
      121 aagcgcctgt cggcataggc cttcaacata tcgaactgaa tgacggtgcc gaagttagcg
      181 ccgcccttat tgccgccgcc ggtcaacgtg ctgtttgtgg aggggtgcgt gcatttcacg
      241 ccccgcgagg ccttcgagca ggccctggct gggacttaca gccgcctcgg ccgtgaggcg
      301 gaaatgccgg gcgagctggg cggcggcgga ttgccgggcg aatgacggcc gcatccgaac
      361 cgcgcgcccc taatacgccg gctgccggcg gtgaacgcgc agttgtttcc acccgaacac
      421 cacgaacgaa acgactacga ccaacgcggc gtcaactgcc cagcgcagca acgcccggcc
      481 cgccagatcc cattggctct cgaccaacgc tgccggcacc agcgccgcgg cggggatcag
      541 ccccagcgcg atcattacgc ccgccgtcag gatcgaacgg ttcgtggcga ccagaaatgc
      601 ccccgccgtg gccgcgacga tcacctcgag aacgctgaaa agagtcaagt tggtccagta
      661 cgcgaccagc ggcggcgtgg ggaggtagcc tccggaaccg ctgagcaacg gttttccgtg
      721 gaagcgcagc accagggcgg cgatcgccgc gccgataaat aacgcggcat acggcagagg
      781 agtgcagcgc aggcccgccc gccaggcggc gctgcgcgcc acgacgcccc gcgcgatccg
      841 gacgagcggc tcaaagcccg gcgcaatcaa cattcccgcg accacgatgt gcaaagcgcc
      901 ggtcgcaaga ccggaaaccg cgatgaagcc ggaaatcgcc attaccagca ggacgtcgct
      961 gctggtgtcg cggactaggg ccgtctcgat ttcctcaaaa ctgcactcgc tcgtgtcatt
     1021 ctggagctcg gcggccgcag ttggcgatat tatgcaaagc ggctgagcgg tgctgagcac
     1081 cgttccgggc ccccggccga cgccttgttc atccagcagg cgcaacgtcg gcatcagttc
     1141 gcggtttctc acttcgatag tgaccagatc gccgggcggc tcaagcgaaa cgcccggttg
     1201 gagacggatg ctgagcgtcc gctcgctttg ctgcagcgca ttcagcagcg gcgttgtcgt
     1261 gtgggatggg acgacaattt gaatgacgcg cgccatccac cggtctccgc ctcattctag
     1321 gtggcgaagc gcgatgacgt ggccgcccgt taatcaacgc agggcggctg cgcagcggcc
     1381 cataaacgcg gacggtcagg gcagcggttt cggctgacct tcctcttttc gagattttca
     1441 agggactagg catcgggctt cagatgggct aagataacaa tggagcaacg tttttgtttg
     1501 acgcccgact acaagccggt cagtacggtc gcgcgagtgt cggtgttcga cgcccgctat
     1561 tgggcgcccg ttggagcgaa tccgcccggc aacgaacgct ggcgctggct tgtatgctgc
     1621 tagccggctg tagcgacccc gttaccgccc caatcgccga agcacgaccc ctgcccgctc
     1681 ccgccaaccc cgcccgaatc gctcaaacgc tgccgttgtc cgtcccggct gatgtgcagt
     1741 tcataatcct gcacgtgcaa acgccacgcg ccgcccgtga ccagctcaac cgcgtctggg
     1801 acctggtacg cgaggacagc gtggacagcg agacggccgt gcggctgtac agcaacggcg
     1861 tccggatggg cgtgggacag attgcccggt gggacgccat caaggacatt ttcaacgcga
     1921 ccgagaaaag ccgcgtgtac gaactgccgc cgatgcacaa tccgccgggc gtgccgctgg
     1981 gcctgcacct cgacgccacg ccgcgcgatc aaacgatctt cttcctcgac gatgatggca
     2041 ttctgaccgg cgacacctgg ccggcgagcc aaaaagtgtt gcggctgacg tatgagccgg
     2101 acccgctgca gcgtgacagc gtgcgcctga tggtcgtacc cgagatacgc caggagctgc
     2161 ctccggccct gacctacact accactgaag gctgggcgac ggggcggcgc cgcgagggcc
     2221 gggcttacgg atcggccgct tttgaaattc ccctcagcac tgaagagttt gttatggttg
     2281 gcccgggcga gaaggccgac gtgtttggca tggttggcgg ggccttcctt acaggactgg
     2341 tcaacgacga gcgttatgat actttcgttt tcatccgagc ggagataagt catggccggc
     2401 ccaccagata gtcgcgccgc ccttgtcggc catctggacg ccgggttcct gcacgggctc
     2461 tttgagagcg tcgggctggc aatcgtggcc tgtgagccgg atggacgggt gattgcgtgt
     2521 aactcggcgg ccgccgattt attcgggcgg gtccagtcaa gcacgcccgg catttacgtc
     2581 gctgagcttt ttcccgagcg cgaccggccg cgccttgagc aacttttagt ggataccacc
     2641 acgacgctcg agccgcgcga gtatcaaacg cagcttgggc gggacgaagc cgggccgctg
     2701 gatttcgcgg tgctcgcgac gccggtgttg gaacacgacg gttcgctccg cggcgtttcg
     2761 ttgtggatgc gcgacattac caagcgtcgc cggctgcagc ggcaactgaa gcgcaacgag
     2821 cgcctgactt acctgggcaa gctggccggg gcagtagccc atcattacaa caacctgctg
     2881 tgctcgattg ccaccagcct cgaatatgcc atgaacatga acaccttgac cgccatgcgg
     2941 cgcgccgcgc agcgcaccgc agaggcggtc gggcaggccg ccgatatcac gcggcaactg
     3001 ctggcgttcg cgcaggcgga ccaccgcgcc tccgacctgg ccgacctgag cgagatcgtg
     3061 gccgagattc tgcatggcaa tgagcagcga ttcaagaatc agcgcgtcga gctacgtttt
     3121 gattatcagc cgctgcccca ggtgctggtt gaccgcgagc agatgatcat cgttctgaat
     3181 catgtgctga ataacgcgct ggaagctatg ccggacggcg gcgtgctgcg cgtctcgctg
     3241 gcctcgctcg ggccggagat cgtttgtctg tccatcgcgg acaccggacc gggcataagc
     3301 cctgagcata tggagcatct tttcgagccc ttcttcacta ccaagggcgt gctgggcggg
     3361 ggcaagtcgg gcaatgccgg cttgggtttg gcggtcgtac acggactggt gcacgacatg
     3421 cacggcgccg tgaccgcggc caacgtgccg ggcggcggag cccgcctcga cattgtccta
     3481 ccagtggaac gccccgagga tcgcccgccc ccacgctagc gcggcggtca gtggtgataa
     3541 agtgataggg tgataaagga cgatccgcgc aaccccctta tcaccctatc actttatcac
     3601 tccgggcggc cgcgtgtgcc actgcatgaa gcggcagctt caagcagtgc gggctgcggc
     3661 aaactccggc ctactgcgct ggccggcgcg gcggcggcag ctcgatggga ccgaaacgct
     3721 cctcgtgcgc cttgatggtc tgctgtaatg cgatcaacag ccgtttggcg ttggccggta
     3781 tcagcgtcac acggtgcgag acgcgcacga ccggctcttt catattgggt agcggcagat
     3841 tggcccccac gtacagcacc acctcatccg gcgagccggc cagcgcgaac gcattcgcgt
     3901 acacggtctc catgccggac aggtcgaggc ggatctgctg tggttgcggt tggttttgag
     3961 gggtgctgct ttcttcgccg aaaatcatgc cggtgctccg gtaagtacct tcgcaaaact
     4021 cgcacgccag tctaagccgc gccgcaggtt ccaacaagcg gcggcattca ccagagagga
     4081 tcgcgccaga tatcgttaca ttttgatccc ggaatccccc atccctgcct aatccgttat
     4141 gcggtcgtca cgcgcggcgg gcgctgcagg ccggcgcgtg gctccaaccc caggtccgcc
     4201 gtctctcccg ccaaccagtg gcggtagccc agtgccgcaa ccatggcggc gttgtccgtg
     4261 caatacgcca gcggggtcag gtgcagcgtc agcccgcgtt cagtgcacgc ctgcttcaat
     4321 gcctcgcgaa gcgccgagtt tgcggccact ccgccgccca ccactaccgt cgaaacgccc
     4381 gcgtgctcgg ccgccgcaac tgtcttggcc accaacgtct ctaccagcgc ggcttgaaag
     4441 ctggccgcga tgtcggccaa ctgccgccgg ctgagatggg cgacgctgcc gtatttgtgc
     4501 gggccgccgt aggtgtggta gagcacggcc gtcttgagcc cgctgaagga aaagtcaaag
     4561 tgcggcttgt gtatccatgg gcgcggaaag tcgatcgcgg ccgggtcccc ctcgcgggcc
     4621 aaggcgtcga tcaccgggcc cccgggatac cccagttcga gaattgcggc cactttgtcg
     4681 aacgcctcgc ccgcggcgtc atcgaccgtc tgccccagca actcgatgcg caggaagtcg
     4741 cgcaccaggt acagcgaggt gtgcccgccg ctcacgacga gcgccacggc cggccagggc
     4801 gaggtgtcgg ccgtcaacgc cgcgctggtg gcgtgagcct cgatgtgatc gacggcaacc
     4861 agcggcagac cctgtgcaag gctcaaagtc ttagccgcgg ttacgccgat cagcagcgag
     4921 ccgatcagcc ccgggccgac cgtcaccgca atcgcatcca ggtcgctgta gcgcacgccg
     4981 gcctcagcca gcgccccctc gacgatgggc cggacctgct cgacgtgagc acgtgacgcc
     5041 agttcgggca cgatcccgcc atacttggca tgcagcgcca cttgcgagct gaccaggctg
     5101 gagagcacct gccgcccgcc ggccacgacc gcggccgagg tttcgtcgca ggaagtctcg
     5161 atgccgagaa tgagggcgtc cgccttttgc atgccggatt ttactcccgc gcgccccacc
     5221 ccgcgccccg ctgcgcgggc gtctccgcaa gccggcgcta cagaccagcc gcttgggcag
     5281 cggcgtacgt gccccacgcc gcaaagtacg ccaggatggt cattccaaag aacgaggctg
     5341 ccggccaggc gatcgaacgc atttcgcgcc cgatcattat cagcgtgctg ccgcattggg
     5401 cgcacagcgc gaagaacacc atgatcgaca atgctacagg cagcgtgaac aagcgctggt
     5461 ccgtgccgat ccacgtagcg ctttgcaggg cttgatgcaa gctctccgcg ccgccccccg
     5521 aggattcgcc caggttgaac agcgtgccga gcgtggccac caccatctcg cgcgccggaa
     5581 acgacgccag cacggagacg ccgatgcgcc aatcccagcc taggggccgc agaaccggct
     5641 caatcccttg cccgagccgg cccaggtagc tatcgcgaag ctgctgcgcg tcgagctcag
     5701 ccttcagccg ttcgggtccc caatcggcag cctgcgcctg ctgttcgacc acgctgcggg
     5761 cggccgcgtc gtgcggaaag tacgccagcg cccacactac caggttcagg accaagatga
     5821 tgcttccggc gcggacgagg aaactgcggc cgccgctgta ggcgcgctgc aagacggtgc
     5881 ccgggcgcgg cagcttgtaa ctcggcagct cgagcagaaa gccggacgta ggcccggcga
     5941 aggccgtctt tcgcagcagc caggccgtgg gaatggccac cacgattcct accagataca
     6001 tcgccagcat caccagggcc ggcaagccga tcagcccgcc gaaatagtta atgtccggca
     6061 ccagggcgcc aaccatcacc aggtacaccg gcagccgggc cgagcagctc atgaacggaa
     6121 ttaccagcat cgtaacgcag cgctcgcgcc ggtcggcgat agtgcgcgtg ctcatgatgg
     6181 ccgggacggc gcaggcaaac gaggacagca gcgggatgaa cgacaacccg ctcagcccca
     6241 tgccgcgcat caggcggtca accatgaatg ccgcccgcgc catgtagccg caatcctcca
     6301 gcagcgcgat cagggcgaag aggatcatga cttggggcag aaagaccagc acgctgccca
     6361 cgccgccgat cacgccatcg acgagcaaac tgcggaatac gccttcgggc aggagttgcg
     6421 tcgcctgggc gcctaccaat tcgaagagcg cttcgatcgc acccataagt gggcgggcgg
     6481 cgtaaaagat cgattggaag accaggcaca gcacgatcag cagcaaggca aagccgccca
     6541 gcttgtgggt caggacgcgg tcgagccggc tggaccaggt ggatcggccg tgctgcgaac
     6601 gagcgaccac gccttcgagc acattgtcga tccaggcgta acgggcccga atctccgccg
     6661 ccgttccgtc gatttgcgct gtctcaagca gccgccgggc cgcggcaagc tcggcggcca
     6721 agccgccgtg cgccaggaac tcgcgttcgg cataaccctc gttatccagc agaacgcgca
     6781 aactttcgca gcgtgaaagc tgcccgttgc ttgcggccag ttgcgcggca ctctgttcga
     6841 ttacgctcgg caggggcgct ggccgcggtg gccgcagttc tgccaacgcg gcatcaagcg
     6901 cggcgatgaa cggcggcagc gtgcgcttgt cggtcgcgat taccggaatg acccgcaccc
     6961 ccaggcgttc tgccagccgc gctatgtcaa tctcaagacc gcgggcgcgg gccacgtcca
     7021 acatgttgag cgcaaccaca accggcaggc caaaatccag taactggcta agcaggtaca
     7081 ggttgcgccg caggttggag gcatccacga cggccagaat cacgtccggc cggctgcggc
     7141 ctgagacgcg cccggaaagg acgttgcaca cgaccatttc gtcgggcgag gcggccgcca
     7201 ggctgtacgt gccgggcagg tccagcagct ccaggtcatg acggctgccg cggatcgggg
     7261 cctcgccaat gtccacggtt actccgggat agttggccac cttgcggcgg aagcccgtca
     7321 gcgcgttgaa gagaaccgtt ttaccagtgt tgggattgcc gaccagggcg atggtcgcag
     7381 tcttgcaggc ggtcgtcgcg gcgcagcaac agacacccgt cgctcgttcg ccgcaaccag
     7441 tgcaagggtc aaacaaggac aatatcgatt gcggatctgg gctgcggccg gtcgggaccg
     7501 tcacccacgt cagggttcag ctcacagcgg cgatacgtca atcaattcgg cctcgcggct
     7561 gcgcagagac aactcgtaat cacctagcct aatgtgcaac ggatcgccta gcggcgctcg
     7621 gcgcaagacc gccaggacgg cgccttcgat caggcccatt tccatgagcc gacggccgaa
     7681 cgctccgctg tttcgaacgc aggtaacgcg cagcgccttt tcacggggag cgtgtaccaa
     7741 ccccattttc gtagttctcc gtgccgcgaa cacttaggaa accatgcatc ggcgcattac
     7801 atggcaagcc gtgtgccttt gcaggccgtg tgattatatg ttaatcgacc atgctggcga
     7861 cacaagtccg cggcatcgtc ctataactct ccggccgacg ggtcgttgta atggccggcc
     7921 gccaattaaa cacggcgagc ggccttttct gcgcctgagc cccgctggat tacaaatttt
     7981 ttaggaccgc gccttcaaga aaatcgttgc tgtgactccc ccgttatcgc tatattgcgg
     8041 ctgactggta agagccggtt tcctagctgg gcgaggtccg ctttgggccg aagccttacc
     8101 gagtcgcgct ctgagagcaa ggaggagagg aatgctcaag tcgcgcgttt ttttgtatct
     8161 ggcatttttg tttttggttt gtcccctcgc tactgctgag atcatcgact ggacctgcga
     8221 tgatgacggc gatggggcca tcgtgatgga gtccgcgctc ttcggcccat cagccgaggg
     8281 gtactcgttg gagttgatct gcactcagaa ttggtatccg ggccacctgc agggtgactt
     8341 cataacggat acgcccgagg atccgactgt ttcgctagtt cagatcatca ccaacgacac
     8401 tgacttcgtc tggactgact accacattac aatcggaatg ccccagccgt tctcgatcgt
     8461 cggggcattt gggcctaccg attggacttg gaacgtcacg ccggccacgg acgggctggc
     8521 gctgccgggc catcccagcc cgggcacggg ttgggtcggc acgattgact actttgccgg
     8581 gtcgcccatt cccataggcg gttccggtac gttcggcttc gtcgtgtctt ttgaaggcac
     8641 ggtgagcttc tgcaccgagc agattcccac gcctgagccg accagcgttt tgttgttggc
     8701 gcttggcgcg ttgaccgtcc tccgccggcg ataggccgcg ggattggatc aaaaaaacat
     8761 cgaccggcgc ggtgaagccg cggcgcgcgg acccgcacta ccttgcgtga atgtctgcgg
     8821 cagtggccgg gacttttcgc gcacggcgct aacgcccaac ctctgcctga tatccgaagg
     8881 ggaatactga tgcgctgagc ccgcagcggc tgcagcgctt cgacggtttg acgttgccga
     8941 ggcgaccgag tcgcaaactg ctcatcaccg gagccggtgc ctcggctcta cgctctattc
     9001 ggggcggcgg gatttgaacc cacgacctcc tggacccaaa ccaggcgctc tagccaggct
     9061 gagctacgcc ccgcattgat cgcacatatt aacgccgttg aaccacggcc ggaatgggcg
     9121 caaaaattgc ggacttgggg aacagtgctg gccgagccca aaaaatggcg atttgccgac
     9181 caccaggctc gccgaaaccc cgtcgacgga ccgctttgaa gggccgggcg catgttttct
     9241 taccgaccag aatttgtcct aaagccacac gcgaagctgt acgagaattt atccggaaga
     9301 ttgtggaaag caggccggct ggggcctata accattacga aactcctcct cctcctcctc
     9361 aaggcccgca acaccccccg cgggccttga ctttgcgccc accgctgctc cccccaagcc
     9421 cactcgtagt cttctgcccc taagcccgcc ccggcggttc tgccgccaaa gccccgctag
     9481 cgagcctcct cgcccgtcgc cgagtaaacc gcgcggccgc gcttaagtac ctggcgtact
     9541 tgtggccgac ccaccgcgta aaaccactca tccagcgtcg gggcatcgag cacgaccagg
     9601 tcggcctcgt agcccgcggc gatcgcaccc agacgttcgg ccgcccccac ggccacagcc
     9661 gcgtttgcgg tacacgcggc cacgatctcc gtcggccgca ggcgcaattg gcaggccgcg
     9721 atgttcatga ccagcggcag ggactcgatc atgcaggacc cggggttgaa atcagtggcg
     9781 attgcgaccg gcaggccggc ttcgatcagg cggcgtgcgg gacaatgctc aatgcccagg
     9841 aaaaaggacg tgccaggcag cacgaccgcc acggcgccgc cgctcttcaa agccgtgatt
     9901 ccggcgtcgt cgatgcgttc taggtgatcc gccgaagcgg cctgcaactc gcccgccagt
     9961 ccggaggccc caatctgggc caactcgtcc gcgtgcagct tgggtcgcaa tccagcggcc
    10021 gccgcccggc tgagcatggt gcgtgcctgt tcaaccgtga acgcgccgcg gtcgcagaag
    10081 acgtcgcaga agcgggccag cttctcgcgg gcgattcgcg ccagcagctc cgggctactc
    10141 atgagctcga tatattcgtc gctccgcccc tcgaattcag ccggtaacgc gtgggcgcca
    10201 aggtaggtcg gaacgagttc aatcggctgc agggccgcca ggtcgcgcac ggccgctagc
    10261 tgcttgattt cgtgctccgg cgacaggccg tagccgctct tgcactccac cgtcgtcacg
    10321 ccgtagctca gcatgcgccg caagcggggc aggttttcat cgacaagttg ctgccggctg
    10381 gcttgacgca cggcggcggt cgtcacgcgt ataccaccgc cggactgcat gatcgataaa
    10441 tacgtttcac ccgccagacg ccgcacgaac tcaccgctgc gattgccggc aaaaggaatg
    10501 tgcgtatggc aatcgatcag cccaggaatc acgcaaccgc cggccgccga gaggcggcgc
    10561 gcgtcgctta cccgttcgcc ctcgtgcgtg gccggcgcct cccgctgcgg accgaaccag
    10621 gcaatgcgcc cgtcacgaac aagcaaggcc gcgtcttcga tcaacggcca ctcacgcatg
    10681 gcggcgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtat cgaacaacgg
    10801 aaccgggctg ctctcgggct tgatcgccag acccagttcg gtgcaaccga ggatcacccc
    10861 gcgcgcccca ttttggacaa gcgtgccaac gatgttctgc aactcggccg cgccagtcgt
    10921 gtcgccgcgg cacaggtcat caaagatgat gtcgtgcacg cgttggcgct gcggggcgga
    10981 gggagtaacg acctcgaggc cgaacttgct ttccagccgg ccgcgataaa agtcatcctc
    11041 catcgtgtat cgcgtcccta gtagcccgac gcgtcgcaag ccggccacgc gaatccgctc
    11101 ggcggtcgcg tcaacaatgt gcagcagcgg aatccgcacc gcggcctcga tcgcgcccgc
    11161 aactttgtgc atggtgttcg tgcacaggac cacaaaatcg gccccggccc gttcgacggc
    11221 ctgtgcggcc tccgccattg cccgccccgc cgtggcccag tcgccggcgc tctggagttc
    11281 ctcgatctcg gcgaaatcga ccgagtacag cacgcatttg gccgagtgca ggccgcccag
    11341 ccgcttcgca acgcgctcgt tgatgatgcg gtaatacgcc agcgtggatt gccagctcat
    11401 tccgccgatc aggccgatgg tcttcacgca cgcatgatag cctgtcgtcc gcagcgccga
    11461 gaaccgcgcc gcggccggcg gcctgctatt ccgcacgtcg ttcgcggccg ataagatcgt
    11521 tttcaactgg ctcggaacgg cgcgatgccg caagagccca caggaagaaa gccatgccag
    11581 aagaaatcat tcaatatgac gactttctca aactcgacct ccgcgtcggc acgatcaccg
    11641 cggccgaagc acatccgaat gctgacaagc tgctcgtgtt gcaggtggac ctcgggcccg
    11701 agcagcggca gctcgtcgcg ggcatccggg cgcactacga accggcgcag ctcgtcggca
    11761 agcaaatcat cgtggtagcc aatctcgcgc cacggatgat gcgcgggctc gagagcaagg
    11821 gcatgctgct ggccgcgtcc acgccggaca agtcgcaagt cgtgctgctg acgacagaaa
    11881 aaccggtccc gccgggatcg acggtgtcct aggggcgtgc gagcatctgg accggcacgc
    11941 gggcgccagg gtgataaggt gataaagtga tagagtgata aaggggattc cgcggaccgt
    12001 cctttaccac tttatcgttt tatcacctta tcacatcacc atgttacgtc gccgaggtgg
    12061 cggccgcggg ctcgggttga gccgcgtcct cttcgggccc gagttcgcat atgggcgtgg
    12121 cggcgcgcat gaagcgcttc ttgaattccg ccggcgacat gtgcgcctcg actgccaggc
    12181 gcgccagcat gatcgggtct tccaaatcag aacgctcgag gcgtatctgg taggctcggg
    12241 cggctttgta tttgtcgctc tgcgtgtcga ccagtcgcgt gcggatgcgg ttggtcgcgg
    12301 gatcgaccag gtcgtgcagg tcgagcgcga ccaggttctc gttgaccaac gctacaaggc
    12361 agcccgacgc gcggcgcgtg ccgtcttcga acagccggat gctgtggtaa ccaagactgc
    12421 ggcagtaagc catgtcgtgc ggtgtgggat cggcggagcg caactcgtag ccgatcgtgt
    12481 gcaaaacaag cggcatgttt tcgccgcggg cgcggaagcg ggcctgcact tcctgacgca
    12541 gcatttcagc cagcggaact tccgccaagc gcgggtggcc ggccgcatcc acgggcacgc
    12601 tgcggcccag cagacgctcg agctcttcgc ggtcgcccaa gcgatatgcc agaccctcgg
    12661 caatcagggc cacgccgtcc ggccggccca tgacgcggcg tttgataatg gccccttcga
    12721 gcacgtccgc gatgcgttgc acgctgctcc gctcggtgaa ttcttcggga atcagcgtga
    12781 cggtcgcccc ggcggagatg cccatggaca tcgccagcca gccggcgctg cggcccatgg
    12841 ccgtaacaaa gtaccagcgg ccggtggtct gcgagtcctg catgaggttc ttgatgagtt
    12901 gcgtcccgta gttcagggcg gtcgcaaagc cgaaggtagc ctggttctgc ggcagcggca
    12961 cgtcgttgtc gatggtcttc ggaacgtgga cggttcgcac gcggtcgccg gcggcctcgg
    13021 caatgaagcg cgagctgagg gccgtatcat cgccgccgat ggtgagcagg cagtttatgc
    13081 ccagctcacg cagccggctg acaaccaggc cgaccttgta ggggtcgggt ttgacgaccg
    13141 tgctggtttt cagactctcg ttatcgagga ggttggtgcg cgatatgcgc aggatcgagc
    13201 cgccgtcaaa gtggatgcgg gccacccgct taatgtcgag ccgcaccgag tgcgtcggcg
    13261 gatcgaaatt atcagaacac aaccatttga agccgtcgta gaagccgata acctcaaagc
    13321 cgcggttgat ggcctcaatg acggccgcac cgatgaccgc gttaattccg ggggcgggcc
    13381 ccccgccgac gacgattccg aggcgcttgt gtggcattcg caatcctgtc ctatctccgg
    13441 tgctacacgt aacttagaac cggcttaggt ccgccgggca ggctgctcac cccgacgtcc
    13501 gcaccgggtt ttggaccgca atcaaagata atatcagcct atgcagcggt cgtccggcat
    13561 gcaatcccta gggcgccgat cgattttttt cggccgcggg cgttgcccgg cggcgccggc
    13621 ggcagtatat taatcggctc gacacacgac ttggatagga acgattatgc cgaaacagaa
    13681 ggtccataag ggtctgcgca agcgggttcg cgtcacgccg cgcggtaaag tagtccgcaa
    13741 gcaagccttt cgggggcacc tgatgagccc caaatccggc gctcggcgcc aggcgctgcg
    13801 ccgcatcgta gaagtcaaag gcaagctggc gcagaacctg aagcgggcct tgtgcgccga
    13861 ataggcgcct tgaaggagtc tgagtaatgc ctcgagtact gcccggccac gccacccgcc
    13921 gtcgccataa gaaagtcctc cgcgccgcca gcggcttccg cgcctcgcgc agcacgcttt
    13981 accgctcggc caagccgacg ctgatcaggg ccggcgtcta cgccactcgt gaccggcgcg
    14041 cgcgcaagcg cgagttccgg gcgctgtgga taacgcgcgt cagcgcggcc tgcgacgccc
    14101 gcgggatttc gtacagcaag ttcatgaacg gtctgaagaa cgctaacgtg atattgaacc
    14161 gcaagatgct cagcgaagtg gcgattgccg atccggcggc cttcgacaag ctgaccgagt
    14221 tggcgaagag taaggctaaa gccaccgctt agagcaattc gcggatcgtg gtgcacgccg
    14281 ggcctccggg cccggctttt ttttaggcgg gccagcgtgg ggtacacccc gacgcgcgcg
    14341 gcgcgggcgg gacgggcgag tgcgccactg cctgaagttg aagcttcaag cagtgcgact
    14401 tgaggccaag atcgcccggc gcattttcgt aaacaaacat ttcgctaccg aaggcgcacc
    14461 agggaaccga gaatttcgca ttggagccgg cccgcctttc tgctacactt cgggtagaca
    14521 tcaagtcagg tagtcggaga cgaccgtgcg gaactaggcc ctacaccctg cgtcgaaggt
    14581 tggatgactt tcgcaaggcg ggagggagtg ccgatgccgc tatctccgct cgaatttcaa
    14641 cgtgtttcct tcgtgtatga aaccgcgtca gccccggtct tgagcggcgt caccgccagc
    14701 ttcggacgcg gctggacggg tattgtcggg cccaacggct gcggcaaatc aaccttgctg
    14761 cgcctggctt gcggggaact tgagcccacc agcggcggaa tccaccgccc cggcccggtc
    14821 gcctactgcc cccagcgcac ggatcacccg ccgcccgaat tgccggagtt cttgagcacg
    14881 gacgaaccgc gggcctgcgt gctgcgcggc cgccttggcc ttcaggccga ttggctcaca
    14941 cggtggaaca cgcttagtca cggcgagcaa aagcgggccc agctcgcggt tgtgctggcg
    15001 cgccagccgg cggtgctggc gctggacgaa ccgaccaacc acattgacag ccagacgcgc
    15061 gacctgctgg cgagcgtgct ggacgacttc tccgggatcg gtctgctcgt gagccacgat
    15121 cgcgagttgc tcgaccggct atgccggcgc tgcctgctga tgagcgttgg gcaggtagtg
    15181 ctgcggtcag gaaattacag cgcggcacgg gcacagcaac aagcggagga ggcgagcgcc
    15241 cgggcgcagc gcgagcggtt gcagcacgag ctgcgccgca tcgagcgcga agtctctaac
    15301 cgtcggcgcg aagcggcccg cgcggacaag ctgcgctcca agcgtcattt ggacaagcat
    15361 gatcatgacg gcaaaggagc gattaacctg gcccgcaaca gcggcaagga cggcgtcgcg
    15421 ggacgcagaa tgcggcaact cgaaggccgt gcggcccagg ccgcggcgcg cgtggaccag
    15481 gttcaggtgc gggcgatctc aaagatgggt gtagctctcg gcggcgagcg cgcccggcgc
    15541 cccgttttac tccggctgga tgcaggaagg ctggcgctgg gctctgatag gtggctggtt
    15601 cacccggagc tggtaattct tgcggatgca cgtatcggcc tgacgggaaa aaacgggagc
    15661 ggcaagagca cgatcgtgcg tcacatagtc gatcgcctgg atctgccagc cgggcgggtt
    15721 gtttacctgc cgcaggagct ggacgctgag cggggcgcaa tagcggccac cgaactgcgc
    15781 aagctgccgg accaggtttt gggcgagacc ctgtcccaca tcagcaggtt gggatccgac
    15841 ccgcgcgccc tccttgaaac ggctcaaccc agtccgggcg agaccaggaa gattctgctg
    15901 gcgatgggca tggcgctcca gccgcacctg atcatcatgg acgagccgac caaccacctg
    15961 gatttgccct cgattgaatg cctggaagca gcactggcgg attgcccggc cgcgctgttg
    16021 ctggtcagcc atgaccgccg ttttctgaaa cggctcacgc gcatccggtg gcacattgtc
    16081 cgcgatgagc gcgactcgcg cgtggaaggg ctgcacgtgc actgaggcgc gaacgcgaca
    16141 gacgcgggta cgatagcaat aattcgggta tggcagccga acctccgtcg ggcaacttcc
    16201 cgccgaaacc gcagtcgcga ccttcgccgt catccgcccc gcacgcgctc tccgcagagg
    16261 acgtgttgcg cgattacgcg agtgacgcgc actccggcct gagcgctgcg caggtacagg
    16321 agttgcgaaa ccgcttcggc ccgaatgagt tggcgcgcgc ggtgggcgtg ccatggtggc
    16381 gccgctttgc cgcgcagttt gcggatctga tcatctggat actgatcgcc gctgcgctga
    16441 tttccggcgt tgccggggag tggctggacg cggtggtcat cctcgccatc gttgtgctta
    16501 atgccgtgct ggggtttgtc caggaggagc gggcggaacg agccctggcg gcgctggcgg
    16561 agatgtccgc gccgcaggcg aaggtgattc gaggggggcg gttgcagact ctgccggcgg
    16621 cag
//
LOCUS       DAOSYS010000051        16483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2921_length_16483_cov_5.056854, whole genome shotgun sequence.
ACCESSION   DAOSYS010000051 DAOSYS010000000
VERSION     DAOSYS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16483
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_16483_cov_5.056854"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..4440)
                     /locus_tag="PLU99_04885"
     CDS             complement(298..4440)
                     /locus_tag="PLU99_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOO16426.1"
                     /translation="MSPDRWQLVQALFEEALKLAPKQRDEFLTQACADDAELRQEVTT
                     LLEHYTNLPTNFMQPPKPPESPIPCSGVAAPDSLIGQQIGRYTVRSVIATGGMGTVYE
                     AVQEQPRRVVALKVVNRSLLSASSMRRFQFEAQVLGRLRHANIAQIYEAGTHGDGRCS
                     LPFFAMEYVPGARSITAYAAEKKLSTPERLRLFARICDAVDHGHQRGVIHRDLKPANL
                     LVDSSGEPKVIDFGVARATDSDLALTTHQTNVGQLVGTMQYMSPEQCDADPHDLDTRS
                     DVYSLGVVLYELLTGELPYHTTGSTIAQLTHLIKEQAARRPSRLNPRLRGDVETIVLK
                     ALEKDRERRYRSAAALADDIRRYLEGRPIAARPPSLAYVASTFVRRYPWQMVATGLAV
                     LLLFSGLIGFTVTERLGKTAAEKERIAAEQKQQLAQRISALRGAETALLAYDVGTARR
                     LLETAEASGTSWELRHLKSRLDQSAATLAQLPHPVRQISVDPQARFVAVCLEGQPEVE
                     IRAFSDGRVLARLDLGGHVPLTSAIDSDGVLLAVAAAGHSDAAVGHIWLFDLSVSNAV
                     LLEHWVADRQSTVSSLAFHPTEPVLASASGNPGAVRLWRLERPPARHASDENYIREPL
                     ATLLGHGGPVQTLAFSRSGRWLASAGKDSTVRVWDFRAAAEGNPSAAAILCGHSWSVS
                     CLDFSPDEGRLASGGVDGTIRIWNLETALAGSRADQYLYGPDVQIDVLYGHDAPVRAV
                     RFDDTGLRLVSGGDDRALRLWETNESATVPDPGSERRELRVPRRRLLSALRGHQGPIV
                     GLAHVLDGRIVSASLDGTVRLWWPEGEDIPRLRGHYAGVRAVTFTPDSAYVISADADD
                     GIIIWDANLCVPVARRHVRLGEGLAGILCWNTDTLPARTLLAVATSGHGVGSKEGGRV
                     ILWDVSEPREPQCISDVAIGAGEAGGTVCALAISPDKRRLAVGDEHGTLRIWPVSENG
                     LDAGAGQLMKLADQPVSALAFLSPGAQGSALHSTSGRQTEEGDFILAACGYSEDAPAR
                     TGTLCIWDIAENRAVWTAAQSLPGGYVSALAVHADGKQVATASHNPQSGTFPICLWYV
                     DWASGLPRMTCEQTLEGHTDAISALAFHPAEPRLASGSRDQTIRIWDLATQVEVATLF
                     GHKGSVRGLAFDANGERLASASAGSLGSDNVVWLWEAVAPQRVRQYAGERAVYSRAYR
                     EVHQAVRINLPSLQKAEERLADLRSRSPWLQRVHEVAARHFVDFASRPAWLNAGAWDV
                     VSRENLPAEQYEQALRWAQRAWTIAPWDGQVLNTLGIALYRCGRYEEAVEALKNAGEL
                     NNWHPVDHAFLAVAYHRLGDSTAAREELERARQRSAEVGLSQLPELRQLMQEAEQLIE
                     GR"
     gene            complement(4446..5039)
                     /locus_tag="PLU99_04890"
     CDS             complement(4446..5039)
                     /locus_tag="PLU99_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO16427.1"
                     /translation="MSGEQPGPVTQLLQAAGEGDGAAREKIWAMVYSELHRLAQQQLA
                     GDALRRGLQPTALVHEAYLRLVGGAEVTWSNRRHFFAAAAQAMRRIRIEDARRQHRLK
                     RGGGQRPAALLDDPAQSASDPLEMLAIDEALHKLEQQDPRKAEIVTLRFFAGLTVDET
                     ARVLEISPRTVDAEWRFAKAWFHRELNAGERPPGTPE"
     gene            complement(5293..6531)
                     /locus_tag="PLU99_04895"
     CDS             complement(5293..6531)
                     /locus_tag="PLU99_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOO16428.1"
                     /translation="MRLQSITLTVVLGLASAVLAQNAPPPARPDAQDQARQNRPTEKQ
                     AGPNQPGLNQPGPNQPGQNQPVENEKGKNPPPVRPPEDQPRGPGAMLDRMGSRMIQTL
                     NLTPAQAEQVRTIIDELRPDFEAAEPRELMLKLKEAQAAGDEQQIAALEAQMAEAAAK
                     QDALRIEFFDRVEPILNPQQQQGLEQLRSRFSGERRGAVRLEGMIERLPEELQLTPEQ
                     RQKYDELAAAWRAARDQNREQRRALTRELTEAQKAGNEELLEELRQKLQEVRANRPSD
                     AFYAGLEKILTKEQSEKLSEMRGVWGGPGERQRGLRVRTVVRAAERLDLTKEQREAIR
                     EITRQAMRQAREAKPEERAQTESQIAERLKKDITALLTPEQVTQFEQALKEGGRRGPR
                     RPLPQTQPAPEGNQPQPKQP"
     gene            6948..7175
                     /locus_tag="PLU99_04900"
     CDS             6948..7175
                     /locus_tag="PLU99_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6978..6980,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HOO16429.1"
                     /translation="MNISITYCGVUNYLPKAASLAAEIKREFNVHPKLIEGSGGVFEV
                     KADGRLIFSKAETGRFPEPGEILSALGGTKK"
     gene            7226..8140
                     /locus_tag="PLU99_04905"
     CDS             7226..8140
                     /locus_tag="PLU99_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008699252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO16430.1"
                     /translation="MRVAVTGASGFLGRYIVNLLLERGHECRCWFRPASDRTGFEEVG
                     GQLEWLPGELGDREAGRLLVRDCSAVVHAALHHPGRRFAGGSQQALIEFAQKNIVGTL
                     ELIEAARQAEVPRFIFISSCAVHDRILQDRELDEFHPLLPYTHYGAHKAAIEAFVRSY
                     GYGQGYPICALRPTGIYGLANPPEESKWFDLVRRVARGEAVTVSGGGKEVHVADVARA
                     VELMLSTEEKNVKGEVYNCYDRYISEYDVAVTARELAGSTADIAGEQKQPLNEINTDR
                     IRALGMQFGGWPLFEQTIGQMVDVVRGG"
     gene            9020..12448
                     /locus_tag="PLU99_04910"
     CDS             9020..12448
                     /locus_tag="PLU99_04910"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO16431.1"
                     /translation="MCTLLLVFAVSGAPAFFGCMHEAARQAELNQETGGEDALATDFG
                     QSAELAGYGGSDSWIGNLGASLDGEGDLGPDPAAANAGPTAETGDYDEPVSETVHGDY
                     ADEEAGAEPANPIAVPGEEELDIESPEPALACVATSTTWRNFPFSAQTGVFTATFSVE
                     PNTASMDGVIALAGRTVSGYGDAAVSIRFGPNGRIAARNGDAYVAETQIPYAPNQVLL
                     FRLEVDAAARRYSAYVTPQGGAEAVLATGYAYRTEQAGCSVLDHWACVSAIGSLTVCD
                     FELQGTPPPPGCATTTSGWQNFPIDSQTGRFIATFAATPAAAGMDGVIALAPNEVSGY
                     GNCAVLVRFAPNGRIDARNGGTYSAVTAIPYTVNAEYLFRLEVDCATKRYDAYVTPPG
                     GTPAVLASGYAYRSEQSGCSVLNNWACISAVGAIEVCDFAIDSDDPQQPPPPPPPDCA
                     VATTSWQNFPMENQTGAFTVTYTATPSAAGMDGVVALALNDVSSYSNGAVQIRFAPNG
                     RIDARNGSAYAAATNVPYTPGAEYSFRLAVYLPTHTYSAYVTPPGGNELLLASNYGFR
                     TEQAGVAALNHWACIAAVGGIEICDFAIGPAANQPPIANAGPDQTVVDADGSGSQAVT
                     LSGSGSYDPDGSIVSYVWREGATTLASGVSATVNLAVGSHTITLTVTDDDGATATDTV
                     AVSVVPPGGGTTSWTNGGFDIQISGALFSARWTPYTKDRYVVVAPGANPTVTVIRRSD
                     MRDADGKLRAAGNTRVHVGPLTIGNQQMWTPDWSGYSAQGSFVYSSPFHTGPITAPTP
                     VQIWNQHVGVPFNGSAWLAYGSNALEIWFIPSGMAVGDVPPPDYSNGRIPAGTLVTAP
                     EGTWPALWRNSADRTRLQNAISKFSSQGVAGGSGPFDIRGDGDYYDTLSNWAAKNALL
                     GWRLNSAPHLNAARQQLLALLSVRFYGHEVAAWYRPRCVPSADLLSGYGREQQNVFFN
                     AMIAACALGPNMADALTDEELDAVNAHLYRYAIHDVYASLTNNQYYTADGGINLHYHP
                     IVVTAGWNWNLADAVNVADTLSNPTHTECSYFISGGVGRYALRYCQLGDAGDTSYENV
                     GNSQSVRFWYRFGEFFNRPTEIPAVDEYVRDQWSGDVFGSISDALGALMDSWNP"
     gene            12901..14607
                     /locus_tag="PLU99_04915"
     CDS             12901..14607
                     /locus_tag="PLU99_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16432.1"
                     /translation="MFALLAAIAVASVPDFMGCMHEAARQAESSQYTQDDDSAGADAR
                     DLPQLSGEGPGRDSAAPGPDLVDEDEQPNVSTPTTPSDPSDDPSGPSGPSDPPDPSDP
                     SDPPDTGYVPDPPEQETPAWTNGGFDIEVNGALYAAKWDPYTKDRYVVVERGANPTVT
                     VTRRSDMRDADGKLGTPGNTRIHVGPLTIGDDQMWTPDAAAYAAQGSFVYSGPFQTGP
                     VNGPKPVQIWNQHAGINFNSSAWLAYGSNALEIWFIPADMEPGDVMPPDYANGRIPSG
                     TLVFAPEGIWPALYRDAADETRLHNAVAKFSSQGVAGGGGPFDCRGDGDYYLGLSECA
                     AKNALLGWHLNSEPHLDAACDQLLALLSVRFFGHEVAAWYRPRCVPSADLLTTYGREQ
                     QAVFFNAMIAACALGPNMADALTPEELHAVNAHLYRYAVHDVYASLDETQYYTADGGI
                     NLHFHPICVTAGWNWEIPELIDVADSLSNPTHTECSYFISGGVGRYALRYCEKHDAGD
                     TSYEDVGNSESVCYWYRFGEFFGQPTNLPAVDAYVCDQFGGDSYSSVRDALGALMDSW
                     NP"
     assembly_gap    14841..14940
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14943..16244
                     /locus_tag="PLU99_04920"
     CDS             14943..16244
                     /locus_tag="PLU99_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOO16433.1"
                     /translation="MRKNYILDANVLLHDPQSVFHFADNTVIIPVGVIAEVDRFKKEM
                     TARGYNAREVVRLLDSLRDGQSLAGGISLKAGGTLKVYCESFEAGVGGGGPADQEILR
                     AAKRISQEEPNTPVVIVSKDINLRVRADAAGLRAENYESDQHQLSELYSGHSERELDP
                     ELIAQLKHEGHVEFTDGKLSPNEYLLVRPTDGSRNTVLARVNPAGTGLMLLHEVEEGL
                     CGIKPRNKEQYFAIDALLDHQLQLVTLLGKAGTGKTLLAIAAAMHACLRKKQYRTVLV
                     CRPIIPVGRDLGYLPGDVENKLAPWMKPVTDTVEYLIDSGGPLRGIRDVPGLIRAGLI
                     EIQPLTYIRGRSIARRYVVIDEAQNLTPLEVKTIITRIGQDAKVVLTGDVYQIDNPYV
                     DAESNGFTHLVNRFRGQPLAAHVELRKGERSPLAELAANLL"
BASE COUNT         2955 a         5333 c         5154 g         2941 t
ORIGIN      
        1 agaatgatcc cattgacgtt accaaccgtc acgggcgtga acgcttgggg tacacatgcc
       61 gccaggccgg ccacaaacgc cgcgaacgcc agcaggggca gagtctgccg catcatggtt
      121 ttgctccaca ttcatgtcgt cgatgcgccc tgaactgaag gattctaatc ggcgacgtcg
      181 tgttggcagg ccgctgaagc ggaattcacg aattcgcgag tccacagagg cagtgtcagg
      241 cacaatgtgg gtcggcaact aaaccgacgc gcgcgccggc gattcatggc agagcattca
      301 gcgcccttca attaattgct cagcctcttg catcagttgc ctcagttccg ggagctggct
      361 gaggccgact tccgctgaac gttgccgggc acgctcgagt tcctcgcggg ccgccgtgga
      421 atctcccagc cggtggtatg ccacggccag aaaggcatgg tccacgggat gccagttgtt
      481 aagctcaccg gcgttcttca gcgcttcgac tgcttcttca taacggccac agcggtagag
      541 cgcgatcccg agtgtgttca acacctgacc atcccagggc gcgatcgtcc aggcgcgctg
      601 cgcccaacgg agcgcctgct cgtattgctc ggcgggcagg ttctcgcgag acaccacgtc
      661 ccaggctccg gcattgagcc aggccggacg ggaggcgaag tcaacaaagt gccgcgccgc
      721 gacctcatgc acgcgctgca gccagggcga acgcgagcgc aggtccgcaa ggcgttcttc
      781 ggccttctgc agcgacggca ggttgatgcg aaccgcctgg tgaacctcac ggtaagcgcg
      841 tgaatagacg gcccgctcgc ccgcgtactg ccggacgcgc tgcggcgcca cggcttccca
      901 gagccagacg acgttgtccg atccgagcga acccgcagag gccgaagcca ggcgttctcc
      961 atttgcatca aaggccagcc cgcggacgga gcccttgtgc ccaaaaagcg tcgccacctc
     1021 gacctgcgtc gcgaggtccc agattctgat ggtctgatcg cgcgagccgg aggccagacg
     1081 cggctcggcg gggtggaagg ctaaagcgga tatcgcgtcg gtgtgccctt cgagcgtttg
     1141 ctcgcacgtc attcgaggaa gacccgacgc ccagtcaaca taccagaggc agatcggaaa
     1201 cgtgccggac tgcgggttgt gcgacgcggt ggcgacttgc ttgccgtccg cgtgaaccgc
     1261 gagcgccgac acatatccgc cgggaaggct ctgcgccgcg gtccacaccg cgcggttctc
     1321 tgcaatgtcc caaatgcaca atgtccccgt ccgcgccggg gcgtcctccg aataaccaca
     1381 ggcggccagg atgaagtcac cttcctctgt ctgccgccct gacgtcgaat gcaaagcaga
     1441 accttgcgct cctgggctca ggaaagccag agcggaaacc ggctggtcgg ccagtttcat
     1501 aagctggccg gcgcccgcgt cgaggccgtt ttccgaaacc ggccagatcc gcagtgtgcc
     1561 gtgttcgtcg ccgacggcca agcggcgctt atcggggctg atcgcaagcg cgcaaacggt
     1621 gccgccggcc tcgcccgcac ctatggcaac gtcgctgatg cactgcggct cgcgcggctc
     1681 gctgacatcc cacaggatca cccggccgcc ttccttgctc cccacgccgt gcccgctggt
     1741 ggcgacggcc agcagtgtcc gcgcggggag cgtgtccgta ttccagcaga gaatgcccgc
     1801 caggccctca cccaatcgca cgtgacgccg ggcaaccggc acacacagat tcgcgtccca
     1861 gatgatgatc ccatcatccg cgtcggccga tatgacgtag gcgctgtccg gcgtaaacgt
     1921 gacggcccgc acgccggcat aatgcccgcg caggcgcgga atatcctcgc cctccggcca
     1981 ccacaggcgg accgtgccgt cgagcgacgc ggagacaatc cgcccgtcga gtacatgcgc
     2041 tagaccgacg atcggtccct ggtgcccgcg tagtgccgaa agcagtcggc ggcgcgggac
     2101 gcgcagctcg cgccgctcgc tgccaggatc gggcacggtc gcggactcgt tcgtttccca
     2161 gaggcgcagg gcccggtcat caccgcccga gaccagccgc aggcccgtat cgtcgaagcg
     2221 gacagcgcgc actggcgcat catgtccata aagcacgtcg atctgaacat ccgggccgta
     2281 cagatattga tccgcgcgcg agcctgccag cgccgtttcc agattccaaa tgcgaatagt
     2341 cccgtctact ccgcccgacg ccaagcggcc ttcatcggga ctgaaatcca gacaactgac
     2401 tgaccaactg tgcccgcaca ggatggccgc cgcactcggg ttgccctccg ccgccgcacg
     2461 gaaatcccag acgcgcaccg tgctgtcctt gccggccgag gccagccacc gtccactgcg
     2521 actgaaagcc agcgtctgca ccggaccgcc atgccccagg agcgtggcca gaggttcgcg
     2581 gatgtaattc tcgtccgagg catgccgggc gggcgggcgt tcgagccgcc acaatcgcac
     2641 cgcgccaggg tttccggagg cgctcgccag cactggctcc gtcggatgaa atgccagact
     2701 cgacacggtc gactgccggt ccgcaaccca gtgctcgagc aggaccgcat tcgatacgct
     2761 caagtcgaag agccatatat gcccaaccgc tgcgtctgag tggccggcgg cggcgaccgc
     2821 gagcaggacg ccatccgaat ctatggccga cgtcagtggt acgtgcccgc cgaggtcgag
     2881 ccgcgccagg acccgcccgt cgctgaaagc ccgaatctcg acctccggct gcccctccag
     2941 gcaaacggcc acaaagcgcg cctgcggatc gaccgaaatc tggcgcaccg gatgcgggag
     3001 ctgggccagc gttgcggcgc tttgatccag gcgcgacttc aggtgccgca gctcccaact
     3061 cgtgccgctg gcctcggctg tctccagcag gcggcgggca gttccgacgt cgtaggccaa
     3121 tagagccgtt tccgctcccc gcagcgcgct gatccgctgg gccaactgtt gcttttgctc
     3181 ggccgcgatg cgctcctttt ccgcggccgt ctttcccagg cgttcggtga ccgtaaaacc
     3241 gatcaagccg ctgaagagca gcagcaccgc cagcccggtc gcgaccatct gccacggata
     3301 gcgccgcacg aaggtggacg cgacgtaggc gaggctgggt gggcgggccg cgatgggacg
     3361 cccctccagg tagcggcgaa tgtcgtccgc cagcgccgcg gccgagcgat agcgccgctc
     3421 gcggtctttc tcgagcgcct tgaggactat ggtttccacg tcgccgcgca ggcgcgggtt
     3481 gaggcgcgat ggacgccgcg cggcttgctc cttaattagg tgggtgagct gggcgatagt
     3541 gctgccggtc gtatgatacg gcagttcgcc ggtcagcagt tcgtatagca ccacgcccaa
     3601 agagtacaca tcgctgcgcg tgtcgaggtc gtgcgggtcg gcgtcgcact gctcggggct
     3661 catatattgc atcgtgccca cgagctgacc gacgttggtc tggtgcgttg tcagggccag
     3721 gtctgaatcg gtggcgcggg caacgccaaa gtcgatcacc ttcggctcgc cgctggagtc
     3781 caccagcagg ttggcgggtt tcaggtcgcg atggattact cccctctggt ggccgtggtc
     3841 cacggcgtcg catatccggg cgaacaaccg cagacgctca ggtgtggaga gcttcttttc
     3901 ggccgcgtag gcggtgatgc tcctggcgcc cggaacatac tccatcgcga aaaacggcag
     3961 cgagcagcgg ccgtcgccgt gcgtgcccgc ttcgtaaatc tgcgcgatgt tggcgtgccg
     4021 cagccggccc aggacttgcg cctcgaactg gaagcgccgc atcgacgagg ccgaaagcag
     4081 actgcggttt acgaccttca atgccaccac acgccgcggc tgctcctgga ccgcctcgta
     4141 cacggtgccc ataccgccgg tcgcaatcac cgaccggacc gtgtaacgcc cgatctgttg
     4201 gccgatcagt gagtcgggcg cggcgacccc actgcacggg attgggcttt ccggcggctt
     4261 gggaggctgc atgaagttgg tcggcagatt ggtatagtgc tccaacagcg tggtgacttc
     4321 ctgccgcagc tcggcgtcat cggcgcatgc ttgggtgaga aactcgtcgc gctgcttggg
     4381 cgccagcttc agagcctctt cgaaaagcgc ctgcacgagc tgccagcgat ccggcgacat
     4441 gccaactact caggtgtgcc tgggggacgc tcgccggcat tcagctcgcg atgaaaccac
     4501 gccttggcga accgccactc agcatccacg gtgcgcggcg aaatctccaa tacgcgggcc
     4561 gtttcgtcca ccgtcaatcc ggcgaaaaaa cgcaacgtca caatctcggc cttgcgcggg
     4621 tcctgctgct cgagcttatg caacgcctcg tcgatcgcga gcatttccaa ggggtcggaa
     4681 gcggattgcg ccggatcgtc caaaagcgct gccggccgct gtccgccgcc gcgcttgagc
     4741 cgatgctgcc gccgcgcgtc ctcgatccga atgcgccgca tggcctgcgc ggccgccgcg
     4801 aaaaagtgcc ggcgattgct ccaggttact tccgctccgc cgaccagccg taaataggcc
     4861 tcgtgtacca gcgcggtcgg ctgaagcccg cgccgcagcg cgtcgcccgc cagttgttgc
     4921 tgcgccaagc ggtgcagctc gctataaacc atggcccaaa tcttctcgcg cgccgcgccg
     4981 tccccctcgc ccgcggcctg tagaagctgc gttaccggac ccggttgttc gccggacatg
     5041 ggcggtcctc cgaggctcag cgggcaatct tactgcgcga ccgttccggc gacgagcctt
     5101 cacgcctgcg cgattgcgcc cgccgtcatc gcccactccg gcatgctccc agtgtgacgg
     5161 gcgcatccgg cgacgtggaa aaaacgagcg gcagatagca tgcgccggca gcctggcgcg
     5221 gcgtcgtttg ctttcgctga cccctggggc tcggtcagca ggagcatcgc cccgctgcgg
     5281 cgcccagcct tttcagggct gcttcggctg aggctgattg ccctcagggg ccggctgagt
     5341 ctgtggcaga gggcggcgcg gtccgcggcg accgccttct ttgagggcct gctcgaactg
     5401 tgtgacctgt tcgggcgtca gcagcgcggt gatatccttc ttgaggcgct cagcgatctg
     5461 cgattcggtc tgggcgcgct cttcgggttt ggcttcccgg gcctggcgca tcgcttgacg
     5521 cgtgatttcg cgaatcgctt cacgctgctc cttcgtcaag tccaggcgct ctgcagcccg
     5581 caccacggtc cgcacccgca agcctcgctg ccgctcgccc gggccgcccc agacgcctcg
     5641 catctcactt agcttctcgc tctgttcctt ggtgaggatt ttctccaggc cggcgtagaa
     5701 ggcgtcgctc ggcctgttgg cacggacctc ctggagtttc tggcgaagct cctcgagaag
     5761 ctcctcattg ccggcttttt gggcctcagt cagttctctc gtcagggcgc gtctctgctc
     5821 ccggttttgg tcgcgggccg cacgccacgc agccgccagt tcgtcgtact tctggcgttg
     5881 ctcgggcgtg agctgaagtt cctccggcag cctctcgatc atcccttcca atcgcaccgc
     5941 gccgcgccgc tcgccgctga accggctgcg gagttgctcc aggccctgct gctgctgcgg
     6001 attgagtatc ggttcaacgc ggtcgaagaa ctcgatccgc agcgcgtctt gcttcgcagc
     6061 ggcctccgcc atctgggctt ccagggccgc aatctgctgc tcgtcaccgg cggcctgggc
     6121 ctccttcagc ttgagcatca gctcacgcgg ctcagcggct tcgaaatccg gcctgagttc
     6181 gtcgatgatc gtacgtacct gttcagcttg agccggtgtc aggttcagcg tctgaatcat
     6241 tcgcgacccc atgcggtcca acatcgcacc tggaccgcgc ggctggtctt caggcgggcg
     6301 aaccggcggc ggattcttcc ccttttcatt ctctacgggc tgattctgcc ccggctgatt
     6361 tggtcccggc tgatttagtc ccggctgatt cggtcccgcc tgtttctcag ttggtcgatt
     6421 ctgacgcgcc tggtcctgcg catcaggccg cgctggaggc ggggcgttct gagccaaaac
     6481 cgccgatgcc agaccgagca ccaccgtcag agttatcgat tgcagtctca ttcaccacgc
     6541 tcccttttga agcactggcc gcttcatcac gcccgttgaa gcattggccg cttgctcatg
     6601 ctcgctttga agcttcggcc gcccgctcgc gcggcagcgc agccagtcgg gccttttttt
     6661 gacatcgcca gcgcggctct cgacagcgca ccggccgttc aactttccac aacctgcaaa
     6721 gtgtgaccgt ccttacctgc gggcgtcaag gtggcgccag ccgggaaccc atgggccgcc
     6781 gtagttgggc gcatggggtg ggcgtcaacg ggtgccatag tacctctcac gcgtctcaag
     6841 cttcatttgc actgcgcgga cgccggcgct attatcggtc aatccgttgc taggccgtaa
     6901 ctcacgatcc gttgcaaggc cgtaaactca gtagccagga ggacgccatg aacatcagca
     6961 ttacttactg cggtgtttga aactacctgc ccaaggccgc cagtctggcg gcggagatca
     7021 agcgtgaatt caacgtccac ccgaaactga tcgagggctc gggtggcgtg ttcgaggtca
     7081 aggccgacgg ccggctgatt ttcagcaagg cggagacggg ccggttcccg gaacctggcg
     7141 agattctcag cgcactcggc ggaacaaaaa aatagccgct acggcgcaga acaagaaaac
     7201 aggcgttgcg cggcagagga aaagcatgcg cgttgcagtc actggagcga gcggttttct
     7261 cgggcgttac atcgtgaatt tgctcctcga acgaggccac gagtgccgct gctggtttcg
     7321 tcccgccagc gaccgtacgg gctttgaaga ggtgggcggt cagcttgaat ggctgcccgg
     7381 cgaattgggc gaccgcgaag cggggcggtt gctggtccgc gactgctcgg cagtggtgca
     7441 cgccgcgctg caccatccgg gccggcgctt cgcgggcggt agccagcagg cgttgatcga
     7501 attcgcccag aaaaacatcg tgggcacgct ggagctcatt gaagcagccc ggcaggctga
     7561 ggtgccgcgc ttcatcttca tctccagttg tgcggtgcac gacaggatcc tccaagaccg
     7621 cgagctggac gagtttcatc ccctgttgcc atacacccat tacggggccc ataaggccgc
     7681 gattgaggcg ttcgtccgca gttacggcta tgggcagggc tatccgattt gcgcgctgcg
     7741 gccgacgggg atttacgggc tggccaatcc acccgaagaa tcaaagtggt ttgacttggt
     7801 gcggcgggtg gcgcgcggcg aggcggtcac cgtcagcggc ggaggcaagg aggttcatgt
     7861 cgcggacgtg gcgcgcgcgg tagagctgat gctcagcacc gaggagaaga acgtcaaggg
     7921 ggaggtctac aactgctacg atcgctacat atccgaatac gacgtggcgg tgacggcccg
     7981 agaattggcg ggcagcacag ccgacatcgc gggcgagcag aagcagccgt tgaacgagat
     8041 taacacggat cgaatccggg cattaggaat gcagttcggg ggctggccgt tgttcgaaca
     8101 gacgatcgga cagatggtcg acgtggttcg aggcggttga cgagggttga aggtacttag
     8161 cgcccggctc gcacctcaag gggcgctgta atcttgcgca gcggggggct tacaaccgag
     8221 acacctagag tcggatacac ttgagcgctt tgtaatctgc cggagtaaag cccgaacccc
     8281 gcagagttag gcgcacgggc aaccgggaga ggctttgcga aagaggagca ggctgtgcgg
     8341 accggggcaa ggattgctgg gggcccgggg tagcgatgct taggtcccgt ggttgtcagg
     8401 tcgtccaaaa aacctgcaca tgccgtggtc cgaaacgcgt gggcgggcgc ctgctctcgc
     8461 atatcaaatc tcgccggcgc gaaatgacgc gctcccggcc cggcccgtgc caggcaaaca
     8521 aaagggtatt taggttttca gagaacatcc taagcagctc aagagaaggg ctgcttagct
     8581 tccgcaatat tccggtcacg cgcggcgcgc gtgatttcgg aacctcggat ggcacgagcc
     8641 gtttcgcgct cgtcccgctc gatggacttt tctggccggc gctgaaaccg gccgattccc
     8701 aggttgctcc ggccgccgca acggtcggaa tcacctctat cccgccggtt cgccggcacg
     8761 tgggcgcttt agggcgcggt cagacggttg caggtccgaa tcctaggtca aggaccctgc
     8821 cgccggctgc cacatcggct cgcccgccga ttcggtggct cgagtacgca gcggttcgcc
     8881 gccacatggc gcgagctgca gacccaatgc ggagccggag gctccccagg gttgctgtct
     8941 ctgaaacacc ggggaaaaga agatggaggt ctctttgaaa acgcagcccc aaaaaatcct
     9001 gaccctaaaa tgcactggaa tgtgcacctt actgctggtt ttcgccgtct ccggcgcacc
     9061 cgcgtttttc gggtgtatgc acgaggcagc acgccaggct gaactcaacc aggaaaccgg
     9121 gggagaagac gcgctagcca cggatttcgg tcaatcggct gagcttgccg gctacggcgg
     9181 atcagactct tggattggaa atctcggcgc atcacttgat ggggaaggcg atctcggacc
     9241 agacccggcc gcagcaaatg ccggtccgac ggccgaaacc ggcgattacg acgagccggt
     9301 ttccgaaacc gtacatggcg attatgccga cgaagaagca ggcgcagagc cggctaatcc
     9361 catcgccgtc cccggtgaag aggaattgga tattgagtct cccgagccgg ccttggcctg
     9421 tgtcgccacc agcactacgt ggcggaattt cccattctcc gctcaaaccg gagtgtttac
     9481 agcgacattc agcgtcgaac cgaacacggc atcaatggac ggcgtgatcg ccttggccgg
     9541 acgcaccgtt agcggatatg gcgacgccgc cgtatcgatt cggttcgggc cgaacggacg
     9601 aatcgctgcc cgcaacgggg atgcctacgt tgcggaaact cagattccct atgccccgaa
     9661 ccaagttctt ttgttccggt tggaagttga cgccgccgcg cgacggtaca gtgcctatgt
     9721 cacgccgcag ggtggggccg aggccgtcct ggccaccggt tatgcctatc ggactgagca
     9781 agccggttgc agcgtgctgg accactgggc ctgcgtttcg gccatcggta gcctgacggt
     9841 ttgcgatttc gagcttcaag gcacgccacc accgcctggg tgcgcaacaa caaccagcgg
     9901 ttggcagaac ttccccattg acagccagac cggccgcttc atcgcaacct tcgctgccac
     9961 accggcagcc gccggaatgg acggcgtgat cgctttggcg ccgaacgagg tttcgggcta
    10021 cggcaattgc gcggtgctgg tccgcttcgc tccgaacggc cggattgacg cccgcaacgg
    10081 cggcacctat tccgcagtca cggcgatacc ttacaccgtc aatgcagaat atctgttccg
    10141 gctcgaggtc gactgcgcta ccaagcgcta cgacgcctac gtgacgccgc cgggcgggac
    10201 cccagcggtc ttggccagcg gctacgcgta tcgttctgag cagtcggggt gcagcgtgct
    10261 gaataactgg gcctgcattt ccgcggttgg cgcgatcgaa gtctgcgatt tcgcaattga
    10321 ctccgacgat ccgcagcagc ccccgcctcc gcctccgccc gactgcgcag tcgccactac
    10381 ctcgtggcag aacttcccga tggagaacca gaccggagct ttcacggtca catacacggc
    10441 cacgccatcg gcagccggca tggacggcgt agttgctctg gcgctgaatg acgtcagcag
    10501 ctattccaac ggagcggtgc agatccgctt cgcccccaac ggtcgaatcg atgcgcgcaa
    10561 cggcagcgca tatgcggccg ccactaacgt tccgtacacc cctggtgcgg agtactcgtt
    10621 ccgcttggcc gtctacctcc cgacacacac gtacagcgct tatgtcacgc cgcccggcgg
    10681 gaacgagttg ctgctggcca gcaattacgg cttccgcacc gagcaggccg gcgtggccgc
    10741 gctgaaccac tgggcctgta ttgcggccgt gggtggcatt gaaatttgcg atttcgcaat
    10801 tggaccggct gcaaaccagc ccccgattgc aaatgcgggt ccggaccaaa ctgtggtgga
    10861 cgccgacggc tcaggcagtc aggcggtcac gctcagcggt tcgggcagtt atgatccgga
    10921 cggcagcatc gttagctacg tctggagaga gggcgccacc acgttggcct ccggagtaag
    10981 cgccacggtg aatttggccg tgggtagtca cacgatcacg ttgactgtga cagacgacga
    11041 cggcgcgacc gcgacggata ccgtcgcggt cagcgtcgta cctcccggcg gcggcacgac
    11101 gtcctggacc aacggcggct ttgacattca gatcagtgga gcgctgttct ccgccaggtg
    11161 gactccctac accaaggatc gttacgtggt cgtagcgccg ggcgctaacc cgaccgtcac
    11221 ggtgattcgt cgctccgaca tgcgcgatgc cgatggcaag cttagagccg ccggcaacac
    11281 gcgcgtgcac gtcggcccat tgaccattgg caaccagcag atgtggacgc ccgattggtc
    11341 gggctactcc gctcagggca gcttcgtcta ctcgtcgccc ttccacaccg gtcccatcac
    11401 ggcgcccacg ccagtgcaaa tttggaatca gcacgtgggt gtaccgttca acggctcggc
    11461 ttggctggcc tacggctcga acgcattgga aatctggttc attccgtctg gtatggctgt
    11521 cggcgatgtt ccaccgcctg attacagcaa cggccggata cctgccggca cgctcgttac
    11581 cgccccggag ggtacatggc cggctctgtg gcgcaactcc gcggaccgga cgcgcctcca
    11641 gaacgccatt tccaagttct ccagccaggg tgtggcgggc ggcagcggcc ccttcgacat
    11701 ccgcggcgat ggcgactatt acgacaccct cagtaactgg gccgccaaaa acgccctgct
    11761 cggctggcgt cttaacagtg cgccacattt gaatgccgcc cggcaacaat tgctggcgct
    11821 gctctccgtc cgcttctatg gacatgaggt ggccgcctgg taccggccgc gctgcgtacc
    11881 cagcgctgat ctgctcagcg gctacggtcg cgagcagcag aacgtcttct tcaacgccat
    11941 gatcgcagcg tgtgcgctgg ggcccaacat ggccgacgcg ctgaccgacg aggagctgga
    12001 cgcggtaaac gctcacctgt atcgctacgc catacatgac gtttacgctt ccttgaccaa
    12061 caaccagtac tacacggctg atggcgggat taacctgcac tatcacccga tcgtggtgac
    12121 cgccggctgg aactggaact tggcggacgc cgttaacgtc gcggatacgc tctccaaccc
    12181 gacgcacacg gagtgcagct acttcatctc cggcggcgtt gggcgctacg ccctgcggta
    12241 ctgccagctc ggcgacgccg gcgatacctc ctacgagaac gtgggcaact cgcagtcggt
    12301 gcgcttctgg taccgcttcg gagagttttt caaccggccg accgaaatcc cggccgtcga
    12361 cgagtacgtc cgcgatcaat ggagcggtga cgttttcggt tccatatcgg atgccctggg
    12421 cgcgctgatg gatagctgga atccgtaaac ccgccgccga agcaccggcg tactcgccgg
    12481 ccgctcgcgg cttaagacgt taagcctgga aaagctcgca gggccgtccg atattgggcg
    12541 gccctgcggc tttttggtcc ggacgagcga taaaagcgtg agctgtaagc cacatagctt
    12601 acaggggtta caaatgagac acctggagcg gatctaaact aaaagtcctg taaccgcacc
    12661 cggcgtgtgc ccggcgagac cgcgcaccga tcagcggttc gaagtgcctg gcgagctgcg
    12721 cttcaagaag cgttctgtcg gaccagctcc ggcgagcgtt gagaggagag agccagtgcc
    12781 actgtcacag gtagggcggg acccccagct ttggttctct ctaaaacaca gggggatcag
    12841 aggatggagg ttcgcttgaa aacacagccc aaacgcacac tgactctgag atgcaccgga
    12901 atgttcgcat tgctggcggc catcgctgtc gcgagcgtgc ccgacttcat gggttgcatg
    12961 cacgaggcgg cgcgccaggc cgaaagcagc cagtacaccc aggatgacga cagcgcggga
    13021 gcggatgcca gggacctgcc gcaactgagc ggtgaaggcc ccggccgcga ttcagcggcc
    13081 ccaggcccgg atcttgtcga tgaagatgag cagcccaatg taagtacgcc gacgacgccg
    13141 tccgaccctt cggacgatcc gtccgggccc agcggtccgt cggacccgcc cgatccgtcg
    13201 gatccctccg atccgccgga caccggctac gttcctgacc cacccgaaca ggagacgcct
    13261 gcctggacca acggcggctt tgacatcgag gtcaacggcg ccctctatgc cgccaagtgg
    13321 gacccctaca cgaaggaccg ttacgtcgta gtcgagcggg gggcgaaccc gacggtcacg
    13381 gtcacacgcc gctcggatat gcgcgacgcc gacggcaagc tcggaacgcc cggcaacaca
    13441 cgcatacacg tcgggccgct gaccatcggc gatgaccaga tgtggacgcc cgatgccgca
    13501 gcctacgcgg cacaaggcag ctttgtatat tcgggcccct tccagaccgg acccgtgaac
    13561 ggtcctaaac cggtacagat ctggaaccag cacgccggca tcaacttcaa cagctcggcc
    13621 tggctggcct acggctcgaa cgctctcgaa atctggttca tcccagcaga catggagccc
    13681 ggcgacgtca tgccgccgga ttacgctaac ggccgcatac cctccggaac gctcgtcttc
    13741 gctcccgaag gcatctggcc ggccctctac cgtgacgcgg ctgatgagac acgcctgcac
    13801 aacgccgtcg ccaagttctc cagccagggc gtcgccggcg ggggtggtcc cttcgactgc
    13861 cggggcgatg gcgactacta cctcgggctc agcgaatgcg cggccaagaa cgctttgctg
    13921 ggctggcacc tgaatagtga gccgcacctg gacgcggcct gcgaccaatt gctcgcactg
    13981 ctctcggtcc gcttcttcgg gcacgaagtg gcggcctggt accggccgcg ctgcgtgccc
    14041 agcgctgatc tgctcaccac ctacgggcgc gagcagcagg ccgtgttttt caacgcgatg
    14101 atcgcggcct gcgccctagg cccgaacatg gccgacgcgt tgacgccgga ggaacttcat
    14161 gccgtcaatg cccatctgta ccgctatgcg gtccacgacg tctacgcatc gctggacgaa
    14221 acgcagtact acacggccga cggcggcatc aacctgcatt ttcacccgat ctgcgtgacg
    14281 gccggctgga actgggagat tccggagctg atcgacgtgg cggactcact ttcgaacccg
    14341 acgcacacgg agtgcagcta ctttatctcg gggggcgtgg ggcgctacgc gctgcgttac
    14401 tgtgagaaac acgacgccgg cgacacgtcg tatgaggacg tgggcaactc ggagtcggtc
    14461 tgctactggt accgtttcgg agagttcttc gggcagccga cgaacctgcc ggcggttgat
    14521 gcgtatgtgt gcgatcaatt cggcggtgac agttacagct cggtgcgcga cgcgctcggc
    14581 gcgctgatgg atagctggaa cccgtagcga ttagggttta ggggagccgg ggaacggggc
    14641 gtaacgccag ggccgtccga ctttgggcgg ccctgggtgc tttccgttcc atcgcgggcc
    14701 cgggttggct tccaccgggg cgacacgccc cggctctgct ttcgatcgcc ttcctcacgg
    14761 tcttctggcc cgctacactt cccttgtatg cggaagaact acatcctcga cgcgaatgtg
    14821 ctgctgcacg atccgcagtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 gtatgcggaa gaactacatc ctcgacgcga atgtgctgct gcacgatccg cagtccgtgt
    15001 tccactttgc cgacaacacg gtcatcatcc cggtgggcgt catcgctgag gtcgaccgct
    15061 tcaaaaagga gatgaccgcc cgcggttaca acgcccgcga ggtcgtgcgc ctgctcgaca
    15121 gcctgcgcga cggccagagc ctggccggcg gcatcagcct caaggccggc ggcaccctca
    15181 aggtctactg cgagtccttc gaagcgggtg tgggcggcgg cgggccggcc gaccaggaaa
    15241 tcctgcgggc cgccaagcgc atctcgcagg aagaacccaa caccccggtc gtcatcgtca
    15301 gcaaggacat caacctgcgc gtccgggctg acgcggccgg gctgcgcgcc gagaactacg
    15361 aatccgacca gcaccagctt tcggagctct actccggcca ttcggaacgc gagctggacc
    15421 ccgagctgat cgcccagctc aagcacgaag ggcacgttga attcaccgac ggcaagctat
    15481 cgcccaatga gtacctgctg gtccggccga ccgacggctc gcgcaacacc gtcctggctc
    15541 gcgtcaaccc ggccggcacc ggcctgatgc tgctgcacga agtcgaagag ggcctgtgcg
    15601 gcatcaagcc gcgcaacaag gagcagtact tcgcgatcga cgccctgctc gaccaccagc
    15661 ttcaactggt cacgctgctg ggcaaggcgg gcaccggcaa aacgctgttg gccatcgcgg
    15721 ccgccatgca cgcgtgtttg agaaagaaac aataccgcac cgtgttggtg tgtcggccca
    15781 tcatcccggt cgggcgcgac ctgggctatt tgccgggcga cgtggaaaac aagctggcgc
    15841 cgtggatgaa gccggtgacc gacacggttg aatacctgat cgacagtggc ggcccgttgc
    15901 gcggcatccg cgacgtcccc ggcttgatcc gggccggttt gatcgagatt cagccgctga
    15961 cttacatccg cgggcgcagc atcgcgcggc gctacgtcgt cattgacgaa gcgcagaacc
    16021 tgacgccgct ggaggtcaag acgatcatca cgcgcatcgg ccaggacgcg aaagtcgtgc
    16081 tgacgggcga cgtctaccag atcgacaacc catacgttga cgccgaaagc aacggcttca
    16141 cgcacctggt gaaccgcttc cgcggccagc cattggcggc ccacgtggaa ctgcgcaaag
    16201 gcgagcgcag cccgctggcg gagttggcgg caaacctgct ttaagagggt ggccgatccg
    16261 gttgctggcg aaacgccctg taaaagccgg cttttttggg gatacaaacg cggtctcata
    16321 aaaagcctcg tatttgggtt ggattggcag tttgccagtt tgccaagcgg ttgcgggttt
    16381 ggccgataac atcggcgttt ggtttgcgcc aggggcaggt tgccagtttg ccaaggtatg
    16441 cgcgctcgga tttggcaaag tgccgcgctg cgcgagagca gga
//
LOCUS       DAOSYS010000052        16447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2928_length_16447_cov_5.498231, whole genome shotgun sequence.
ACCESSION   DAOSYS010000052 DAOSYS010000000
VERSION     DAOSYS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16447
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_16447_cov_5.498231"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLU99_04925"
     CDS             complement(<1..906)
                     /locus_tag="PLU99_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO16434.1"
                     /translation="MRNLASTRRFAAVLCTAILVWLSGCAGKGPLRQELADHVERPPR
                     SVVLFLCDGVNADLLEEGCGAGWLPNIEQRFCQGGTRVQNAITIHPSITYASVTTLLT
                     GLEPPEHRIPGNRWFDPQQRLLRDYAYIATYVSVNEDTDAETVYELIAPAYSMSVQSP
                     LRRGVSDNIANWAQSGVRWFFRDYPGVDALTAKTVELVARRANRAGQWPALLTLYFPG
                     LDSVGHERGATSPEYHAALLNLDLHVGRVCDWLEEQGLLSTTYLLLVADHGMIDVDPC
                     RHVDLVQALQQTGASLTHQPCQNVPY"
     gene            1133..1282
                     /locus_tag="PLU99_04930"
     CDS             1133..1282
                     /locus_tag="PLU99_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16435.1"
                     /translation="MNRKWKYVMLAGAGGVLLQLAGCGQIMLEMLISNIVPLILSQLV
                     ASAVA"
     gene            complement(1451..2251)
                     /gene="dapB"
                     /locus_tag="PLU99_04935"
     CDS             complement(1451..2251)
                     /gene="dapB"
                     /locus_tag="PLU99_04935"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOO16436.1"
                     /translation="MSTLRLAIAGCTGRTGSTIVRLAAGDPAFQIVAALTATDDPYLG
                     HDAGTAAGLKPVGLAISDAVSGPCDVLVEFTTPSGTAHWARWCGATGVPLVSGTTGLS
                     AEDQLALHDAARRVPVLWSANMSLGANLLLQLVSQVAGKLGEDWDVEIAETHHRHKAD
                     APSGTAKALLAEVARARGRKPAEIAAFGRSGQCGPRAAGQVGVHAIRMGEVVGEHVVA
                     FASANETLTLCHQASSRDAFAAGALTAARWIVNKAAGYYGMRDVLAGG"
     gene            complement(2248..2556)
                     /locus_tag="PLU99_04940"
     CDS             complement(2248..2556)
                     /locus_tag="PLU99_04940"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16437.1"
                     /translation="MTRLEQLQKLVAAAPHDPLTHYAVGLEYFNLGQFAEAVAAFQKA
                     LAADPKYVAAYYHKARAEIRAALQAEARQTLTAGIEAAQAAGDAKTTREMKELLDTIS
                     "
     gene            complement(2594..3274)
                     /locus_tag="PLU99_04945"
     CDS             complement(2594..3274)
                     /locus_tag="PLU99_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16438.1"
                     /translation="MRFVDGRLLVDLAWPGITATALMVVVLLGLAFQAGKRSAASAEP
                     DPIEQTVEQTIAAAAPDPASLLPPELRPPARDLPAPRPAERVQVPVAPAPRAAEPRPE
                     PAPPEPPPQQENQPAFSFQPGYHYVVVQHFRLEHKDAAFDAARFLQANGVDAVVVVKK
                     ADVEVVATTPFLLKQDNRAARAAAQKACDALKEKIRTLGREFAKQQAAAKRPQYALDQ
                     PAERLRLK"
     gene            4188..5279
                     /locus_tag="PLU99_04950"
     CDS             4188..5279
                     /locus_tag="PLU99_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOO16439.1"
                     /translation="MSGTHLPHAPRCYLQSIGTAVPARSFTSEQTVAEFGAVYTEPRS
                     QRLLRRIVRLTGIENRYLAALDFLDRNGDGPPLYVPAAELPGGPGMGVRNARFAEAAG
                     GLVRQAIGELAPGLLESVDALVTVSCTHASAPGLEAAVYDQPVVCRAAHRWNLGFMGC
                     SGGLAALRLVQEMSPRGHTALIVACELSSLHFQYSTELDQMTANVLFADGAAAAVISA
                     EPSPVRVVDCRCATLPEAADQMIWFADDRGLRLRLSQDLPDTLAAALPDAVRTFLGDN
                     GLAVSDVKHWLVHPGGPQILDAAGAALGLPAAALELSRSVLRRYGNMSSATILFILRA
                     LLAQRPKGLCVALAFGPGLTIELALFQMR"
     gene            5698..7056
                     /locus_tag="PLU99_04955"
     CDS             5698..7056
                     /locus_tag="PLU99_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16440.1"
                     /translation="MSRAATAQPKFATIARSLRSHNYRLFFFGQGVSLVGTWMQRIAL
                     SWLVYRLTGSEALLGIVAFAGQIPTFVLAPLGGVLADRWNVHRAIVVMQVLGTIQALA
                     LAVLALTGVITSWQIVLLSVALGVINAFDIPLRQTFVVEMVERPEDLPNAIALNSFLV
                     NGARLLGPSMAGLLIALVGEGICFLINGLSYIPVILALLAMTVAPKPKPRRTDVLSGL
                     AEGFSYAFGFRPIRAALTLLAVVSLVGMPYSVLLPVFAEDILHGGPNTYGFLMGASGL
                     GAIAGALYLAGRSTVLGLGGTMARSAVLFGAGLIAFSLSHWLWLSLALMAVTGFGMML
                     QMASCNTVLQTIVEDDKRGRVMAMYTMAFMGTAPFGSLVAGFAAHSFGAPLTLVIAGA
                     VCVAAAGMFLAQLPELRKAVRPIYVRKGIMPDASQGLSGAGASAGMGPADDRSRGGEP
                     AT"
     gene            7137..10181
                     /locus_tag="PLU99_04960"
     CDS             7137..10181
                     /locus_tag="PLU99_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16441.1"
                     /translation="MVAMVLPLAVAVLIVNLPAIHGLGWPRYVLNCLIWLTTLAILVH
                     DPQMRGRREDSADAAEARRRERKPLLKLRSDRTILAALGVVALLSLLSYYDFGRFHGG
                     GRFVHYHDLYHYYMGSKYFPEVGYEGLYAATHRALVENEPAMSGGISLVKNLRTYDIE
                     NARISWQRSDGVTARFSDSRWEQFKHDVRFFQARLAPAEWQVLLVDHGFNATPFWTGV
                     GAALSSRLPLNSATLLLLALIDPLLVAAALVLVWLTFDARTGLLAVIFLCANFFGVFD
                     ITGGAFLRQLWLAALIGFVCCYRRGWMWPAAALLAVAALDRVFPAVFALPAAVLLIID
                     TVRRRSAGRVQARFLAAFVAFAAGLGLTVCLTVGGPRSWTDWYDKISAHNRWFYTNQL
                     SLRNLFTVNPAQSLRTISEGWNEGLWLRERETLDARTRNTLLAGRTVLLGLLIATVAK
                     RKDALASLALVSFVPLVLFYPADYYCVFLVAAILCWRGNRGLALALVGLQALFWLVNI
                     PLSSGVCLELLHWVISLCLIVVFVVFLGRELLNGGQDQRARRKAPLPLLAGAAAILLA
                     GFVADTRAARRQPLPFELDLTPDDVLLAENLQPGLEHMASWGNAWSRNDHLLCVTTGA
                     AARAVVRVPAPSDGMYSLAVTYSTGPPFGIVTLAINGEQPISTLNLYAPNVGVRTEVY
                     DEVALKGGDNEFSFAVTGKDRAAKDQFFAVDTIKLDYAAPREASSSAAARRAALERAV
                     AWLMAHPADSFDGGRPAICKEIAVLHSVLSTPQLAEWGSKYMPELQQRIERLNTHCGP
                     RLHLHEYDGMLLAGHVARQFNLEFIALAPVAEQLKPWIESVYADRSAAQLLFAGEYFN
                     RLYPEQAVAIDVEQSLVYREYTERKLAQVLAGDVDRNKTRAVMAAISALSQDVCGLTE
                     FGRLAPPAGYLADQEFWGRLCEDGLRWSMQTGDLLTLARVVQLAECLRVAGAVPGLPA
                     AVDLLVEHQRADGSFGAITCDNPNPYREGVLNAALALKASLR"
     gene            complement(10252..10479)
                     /gene="infA"
                     /locus_tag="PLU99_04965"
     CDS             complement(10252..10479)
                     /gene="infA"
                     /locus_tag="PLU99_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOO16442.1"
                     /translation="MAKADAIQMEATVVKALPNAMFLVEADVGNTKHEVLAHVSGRMR
                     KHFIRILPGDRVTVEVSPYDLKRGRIVYRQK"
     gene            10980..11546
                     /locus_tag="PLU99_04970"
     CDS             10980..11546
                     /locus_tag="PLU99_04970"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOO16443.1"
                     /translation="MSPTKTAGVINGVDQDRLQQTVSQMRRDPGSAAFQFRVRNTWLG
                     GAHNRSRIQDFYGGGKEDDTRQKTYVMDCDEPPILLGKDQGPNPVEHLLNALAGCVTT
                     ALVMHAAARGIEIQSVTSELEGDIDVRGFLGLSPDVPRGFKNIRVTMHVQSDAPPEKL
                     RELAEYSPVFNTIANATPVEISIDNART"
     gene            complement(11664..12128)
                     /gene="smpB"
                     /locus_tag="PLU99_04975"
     CDS             complement(11664..12128)
                     /gene="smpB"
                     /locus_tag="PLU99_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOO16444.1"
                     /translation="MAKGPPKAPVIRNKKARFNYEILEKLEAGIALTGSEVKSLRDGK
                     ASLDEAFAMIREGDVFLRDCNISPYPMATYDQHAPTRERRLLLHRREIRKWEAKVTQR
                     GLTIVPLAIYFSDRGIAKVELALVRGKTHEDKRADLKKREHEREMDRAMKRR"
     gene            12244..12855
                     /locus_tag="PLU99_04980"
     CDS             12244..12855
                     /locus_tag="PLU99_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO16445.1"
                     /translation="MSPDELKRIIARAREGHADAFRDLVEAYKERLFAFVWRMVRDHH
                     EAEDICQAVFVKAYESLDSYSEQYAFSTWLFTIAYRMSLNSLRKKKALSGEVDFARFG
                     CDEQDASATLATSEEARRLRKLIWAAVEELSTPQKSSVLLFYREGKSCHDIGKVLGMP
                     AVTVKSHLHRARERLRKALAAELVDERLDGEYLSGSQMLRAQR"
     gene            12852..13526
                     /locus_tag="PLU99_04985"
     CDS             12852..13526
                     /locus_tag="PLU99_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOO16446.1"
                     /translation="MNCAEFERLIDAYLDGELAGSLRVEFDAHRLRCRRCQLTMIAME
                     SVETVLANDRPLATLSDDFTERVMGAIQSRRPVASRLRPRRIVLAAGLLLQAAALMFL
                     AIHPWHEGGSVSEFEPVPVTPAEPEGVRDLDQVYALVDKKTEERQALVDRIFAKLEAA
                     GMNVATDLTQLVSYASALPVPDDVARASDGMHGANPLNLLIDVFIPGAAPELESTPIG
                     TVEHAL"
     gene            13559..15781
                     /locus_tag="PLU99_04990"
     CDS             13559..15781
                     /locus_tag="PLU99_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16447.1"
                     /translation="MIPISRGILVVAVFLTSTPFTLTGLAAPERDSLAHSAPADVGLY
                     IEGRGVADLLQPLIEPQAWLALAELAGQPARLEEANAWRVWIRQGLRMEPAEACRELL
                     GEQFAFVGEGLGRAQDGVVICRPDSEPEVLLRRWEARPLPDAGRSSIYRLANGLGAGL
                     CDGLLIFGAADIPDGMFSRVIAVGEGPQGPASLAEDAVYRGLLARVPEGPDAILFARL
                     RSSTPGAASQPAMAQAAERDAGRSASTSKPSACGAALPGPLHGASNILLALHRERHLL
                     RFSVVGDAPARCDGTQSGLPELVGYLPERTLAAWGFHLDYRNLPGMLARLPERSILRL
                     TFSMQERSGLIARLSEALDSATCLAVGVVEPPSRTEPAPPVPALAVLVNAVQPEEAED
                     EFETLLKQSISFYGLLALKTGAQVIVPDVHRLEVEGADVGLLDVSGLLAGRPGGAAIA
                     ELHICWALDGGTLIVASHLDWLRQILAARHEVAPGLQKTVALSTRPVPPGTETIIALQ
                     TGPLADLGQLWLDHFQKTAPQILSETWWQAQRPGRGNVHLGLTVTEASKEQRLVVTAV
                     KPDTPADGLLRPGDQIVGCGGDQSRPNAPRRFATSQPIREIRDAIARRPDARWVDLLI
                     ERGGTPLVKRIPLPYFDPIQVLRRFIALGQIFQRVVYYEDVPENPVLSDPVLSAEVSI
                     QTAAAEAATQPAITSSEPPGGRGFLTIELRGSREPLFPFALTPAPVSAPITDQARKLP
                     "
     gene            15890..>16447
                     /locus_tag="PLU99_04995"
     CDS             15890..>16447
                     /locus_tag="PLU99_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16448.1"
                     /translation="MNRIVAAVVGASCLLGTSTWGSCDPTGDYAVPLGSYWHIRPDPD
                     GGFILGGSAVSWNMGLTGVVELWAGRQIVAGRTRDAYFVLFPSYELLFGEDGRLKKDP
                     APVLGKLELFEEREAWERRLSEMDCTDVVLRAPRRWDSPAVRFVVYAAIGGACAAAPL
                     SVLLGAGAARMYYEWRARRNASAREA"
BASE COUNT         2744 a         4908 c         5557 g         3238 t
ORIGIN      
        1 atatgggacg ttctggcagg gctgatgcgt cagactggcc cctgtctgct gcaaagcctg
       61 cacgagatca acatgccggc atgggtccac gtcgatcatg ccgtggtcag ccaccaacaa
      121 cagatacgtg gttgaaagca acccttgctc ttcgagccag tcgcagacgc ggcccacgtg
      181 cagatcgaga ttaagcagcg ccgcgtgata ctcgggcgag gtggccccgc gctcgtgtcc
      241 gaccgagtcc agtccgggaa aatataacgt cagcagggcc ggccattgac cggcgcggtt
      301 ggcgcggcgc gccaccaact cgacggtttt ggcggtcagc gcgtcgacgc cgggatagtc
      361 gcggaagaac cagcgcacgc ccgactgcgc ccaattcgcg atgttgtcgc tgacgccgcg
      421 cctcaacggt gattgcacgc tcatgctgta agccggcgcg atcaactcgt agacggtctc
      481 ggcgtccgta tcctcgttga cggaaacata agtggcgatg taggcgtagt cgcgtagcaa
      541 gcgctgctgg ggatcgaacc agcgattgcc ggggatgcgg tgttcgggcg gctccaggcc
      601 ggtcaacagc gtggtcaccg aggcgtaagt gatggacgga tgaatcgtga tcgcgttttg
      661 tacacgcgtt ccgccctggc agaatcgctg ctcgatgttg ggcagccagc cggcgccaca
      721 tccttcttcc agaaggtccg catttacgcc gtcgcacagg aacagcacta cgctgcgcgg
      781 tgggcgctcc acgtgatcgg ccagctcttg ccgtaagggc cctttgccgg cacagcctga
      841 aagccaaacc agaatggcgg tgcaaaggac cgcggcaaat ctgcgcgtgc ttgctaggtt
      901 acgcaatttc gaatcgcctc tatccacttc ttttaaacgc ccggcgcaca aagtacgccg
      961 cattgtcttc atcgtcgccc gcggaacaag acgccgattg cagccaaacc gcgactggaa
     1021 tgtccggcaa cctccgggtg ttgtgcggcg aatggcggcc gctatagtgg agacactcag
     1081 ccaaccgatg ggtgagtcat ggcgtcagcc ttttggcgga ctggaggtcc tgatgaatcg
     1141 taaatggaag tacgtgatgt tggcgggcgc gggcggagtg ctgctacagc ttgcgggctg
     1201 cggccaaatc atgctcgaga tgctcatcag caacatagtc cctctaatcc tcagccaatt
     1261 ggtggcctcc gccgtggcgt gaccgcccgg cagcccagcg cagcgtagcc cgcaagtggt
     1321 agtacgaggg ctaccctgtg ccggtaaccc gccccgctgc gtgaagcagc ggctttaaag
     1381 accaatcagt gctgcgccgc gcgctccacg tgatcgccgg aaccggcagc cgcatccagt
     1441 ctcacttcgc tcagccgccg gccaatacgt cgcgcattcc gtaataaccc gccgccttgt
     1501 ttacaatcca gcgtgcggcc gtcagcgccc cggctgcaaa ggcgtcgcgc gagctggcct
     1561 ggtgacacaa cgtgagagtc tcgttcgccg atgcgaaggc gaccacgtgt tcgcctacta
     1621 cttcgcccat gcggatcgca tgtacaccta cttgccccgc agctcgcggt ccgcattgcc
     1681 ccgatcgtcc gaaggcggca atttccgccg gcttgcggcc gcgggcccgc gcgacttcgg
     1741 ccagcagcgc tttggctgtc ccgctggggg catccgcctt gtggcggtgg tgggtttcgg
     1801 cgatctcaac gtcccagtct tcgcccagct tcccggctac ctgtgacacc agttgcagca
     1861 gcaggttggc gccgaggctc atgttcgccg accacaacac cggaacgcgc cgcgccgcgt
     1921 cgtgcaacgc caattgatct tccgcgctca ggcccgtcgt tccgctcacg agcggcacac
     1981 ccgtcgcgcc gcaccagcgt gcccagtgtg cggtaccgct cggcgtcgtg aactcgacca
     2041 gcacatcgca aggaccactc acggcatcgc tgattgccag cccgacgggt ttcaggccgg
     2101 cggcagttcc ggcgtcgtga ccgaggtatg gatcatccgt ggcggtcagg gccgccacta
     2161 tttggaatgc cgggtcgccg gcagccagac gtacaatggt cgaaccggtt cggccggtgc
     2221 agccggcgat cgccagacga agcgtgctca tgaaatggtg tccagcaatt ctttcatctc
     2281 gcgtgtggtc ttcgcatcgc cggccgcctg tgcggcttcg attccagcag tgagcgtctg
     2341 gcgggcttcg gcctgcagcg ccgcccggat ttcggcccgg gccttatggt aataggccgc
     2401 gacgtacttg gggtcggccg ccagcgcctt ctgaaaagca gcgactgctt cggcaaactg
     2461 cccgagattg aaatattcca aaccgacggc gtaatgggtg agcggatcgt gcggcgccgc
     2521 ggctacaagc ttttgcaatt gttccagtcg cgtcattaac ggcttccgag gcacgaggcc
     2581 gacttgtacg cgcttacttc agccgcaggc gttcggcggg ttgatccagc gcgtattgcg
     2641 gccttttcgc cgcggcctgc tgtttggcga attcgcgtcc gagcgtgcgg attttctcct
     2701 tgagtgcgtc gcaggctttt tgcgcggcgg ccctggcggc gcgattatcc tgcttgagca
     2761 ggaagggggt agttgcgacc acctccacat ctgctttctt tactacgacc accgcgtcca
     2821 cgccgttggc ctggagaaag cgggccgcgt cgaacgccgc gtccttgtgc tcgagccgga
     2881 aatgttgcac gaccacgtag tgatagcccg gctggaagga aaacgccggc tgattctcct
     2941 gctgtggcgg cggttctggc ggcgcgggct cagggcgcgg ctcagcggcg cgcggggccg
     3001 gcgcgaccgg cacttgcacc ctctcggccg ggcgcggcgc tggcaggtcc cgggctgggg
     3061 gacgtaattc cggcggcaac aacgacgcgg gatctggggc ggcagcggcg atagtctgct
     3121 caacagtctg ctcaatagga tcaggctcgg cggatgctgc ggagcgttta ccggcctgga
     3181 acgccaagcc gagcaaaacg accaccatga gggcggtcgc cgtaatgccg ggccaagcca
     3241 aatccacgag caggcgtcca tcgacaaatc gcacggcaga cgctgttctg atggcctcat
     3301 ttgctgagga ggactcggcc gcggccggcg gcgaagtatg caaagccggc ccagggcgct
     3361 cgactgctgg tcggctcggc ctgtcggcat catggcgcgg gtcgcgccag ccgacgtcgc
     3421 tggggcggcg cgtgtggcgg actatctcat atagaacggg tctcttcctg cgtgggggca
     3481 cgaggagtct ccttcctcag cgggccaagc ggcaggccag ccgccaattt tagaactccc
     3541 gccgcgccgc acaaccccgt gagttggtcc cgcgcgcccc cgtgtgtgcc actgctgaag
     3601 cagacgcttc agcagtgccg agtcgcgata agggtgataa ggggataagg tgatagggtg
     3661 ataaaggtgg tctgcggaag cccggtatca ccctatcacc ttatcacttt atcactctgc
     3721 cgaaggcgtc cggaaccgcg cacggccatc ttttttttgc cgtccgatcc gcccgtcagc
     3781 ggcgcgcaac ctatttcggc tcaaaggctt gtaaacgagg cggaactcgt ccgaaagcga
     3841 ttacagatgc gcctgcgcgt tttttttcgg cccgaaactg gatttcgccg cgcaacgctg
     3901 cctataagaa tagtgaatcc tcctcctcct cttggcccgc gagagcgggc tctttttttg
     3961 gcgcgaaaca gacagcaact ccgcgcaaag aggcgggccc gcgaaagctg cccatgctca
     4021 gggccgtgcc ctggctgcca tccctggcaa ccggcgacgc cgcgccgctg aattccggaa
     4081 cgggactccg cccagccgga ccaacactgc ctcacgcaat gaccacgggc ctagaataaa
     4141 ccgtcttctt ggttcgcggg ctgcgattaa gccatcggag ctactctatg tcgggaacgc
     4201 acctgccgca tgcgcccaga tgctatctgc agagcattgg cactgccgtg ccggcgcgca
     4261 gtttcacttc cgagcaaaca gtcgctgaat ttggggcggt gtataccgag ccgcgcagtc
     4321 agcgcttgtt gcggcggatc gtccgcttga ctgggatcga gaatcgctat cttgcggctc
     4381 ttgatttcct cgataggaac ggcgacgggc cgccgctata tgtgccggct gcggagttgc
     4441 cgggtgggcc gggaatgggc gtgcggaacg cgcgctttgc cgaggcggcc gggggtttgg
     4501 tgaggcaggc gattggggag cttgcgcccg gtttgttgga gtccgttgac gcgctggtca
     4561 cggtcagttg cacgcacgcg tcggcgcccg gactggaggc ggccgtttac gaccagccgg
     4621 tagtttgccg ggcggcgcac cgctggaact tggggttcat ggggtgttcg ggcgggctgg
     4681 cggcgctgcg gctggtgcag gagatgtcgc ctcgcgggca cacggcgctg atcgtcgcct
     4741 gcgagttatc gtcgctgcac tttcaataca gcacggagct ggaccaaatg acggccaacg
     4801 tgctcttcgc ggatggcgcg gcggcggccg tgatctccgc cgagccgtcg ccggtgcgtg
     4861 tcgtggactg ccgctgtgcg acgctgccgg aggcggccga ccagatgatc tggtttgcgg
     4921 atgatcgcgg tctgcgattg cggctgtcgc aagatttgcc ggacacgctg gcggcggcgc
     4981 tacccgacgc ggttcgcaca ttcctgggag acaacggact ggcggttagc gacgtgaagc
     5041 actggctggt tcatccgggt gggccgcaaa ttctcgacgc ggcgggcgcg gcattggggc
     5101 tgcccgcggc ggcgctggag ctgtcgcgat cggtgctgcg gcgctacgga aacatgtcgt
     5161 cggccacgat tctgttcatt ctgcgggcgt tgctcgcgca gcggccaaag gggttatgcg
     5221 tggcgctggc ctttgggccg ggtttgacga ttgagctggc gctcttccag atgcgctgat
     5281 gcggtgcggc gcgttttagc gggtttgctt gaacgcttgg aaggcttcgt tccggcgcgg
     5341 gccgattgcc ggcggtgttc gcagccggca tttttgttga ccagcttctc gaggcgttgg
     5401 caccataccg cggccatcgt gcggcagatg ccggccgttt cggccggcgg aaagtcggcg
     5461 aaagggcaat cgaagtcaca aaattcaggc gtcttttctt ccacggtgta tcactttcgc
     5521 gctgccgtgc gcggcccggt tcgccctgtc gtcgcttctg ccgaccggac ctacgtgaga
     5581 atgttacggc atgttgaagg ccgcgtgaat tccgcaaaac gacaagcgcc ggcatttccc
     5641 gtagaatcac gctattgcgc gtgaagagcc cacgccctga gggtccgcgg aaccaaaatg
     5701 agccgggcgg caactgcaca gccgaagttc gccacgatcg cccgctccct gcgctcgcac
     5761 aactaccggc tgttcttttt cggtcagggc gtgtcgttgg tcgggacatg gatgcagcgc
     5821 atcgcgctga gctggctggt ctaccggctg accggttccg aggcgctgct tggaatcgtg
     5881 gcttttgcgg gccagattcc gacgttcgtg ctggcgccgc tgggcggggt cctggcagat
     5941 cgctggaacg tgcatcgcgc catcgtcgtg atgcaggtgc tggggacgat tcaggcgttg
     6001 gctttggcgg tgctggcgct gacgggcgtc atcacgtcat ggcagatagt gttgctgagc
     6061 gttgcgctgg gggtcatcaa cgccttcgat atcccactgc gccagacgtt tgtggtcgag
     6121 atggttgagc gccccgagga cctgccaaac gcgattgcac tcaattcctt cctcgtcaac
     6181 ggcgcccggc tgctggggcc ctccatggcg gggttgttga tcgcactcgt aggtgagggc
     6241 atctgcttcc tgatcaacgg gttaagctac atcccggtaa tcctggccct gctggcgatg
     6301 accgtcgcgc ccaagcctaa gccgcggcga acggacgtgc tgagcgggct ggccgagggt
     6361 ttctcgtacg cttttggttt tcgaccgatt cgggcggcgc tgacgctgtt ggcagtggtt
     6421 agcctggtgg gcatgccgta ttcggtactg cttcccgtgt tcgctgagga catcctgcac
     6481 ggcgggccca atacctacgg gttcttgatg ggcgcgtctg gactcggcgc gatcgctggg
     6541 gcgctgtacc tggccgggcg gagcaccgtg ctcggcctgg gcggcacgat ggcccgctcg
     6601 gccgtcctgt tcggggccgg tttgatcgcc ttttccctct cgcactggtt gtggctctcg
     6661 ctggcgttga tggcggtgac cggtttcggc atgatgctcc aaatggcttc gtgcaacacg
     6721 gttctgcaaa ccattgtcga ggacgataag cgcggccgcg tgatggccat gtataccatg
     6781 gcgttcatgg gcacggcgcc gtttggcagc ctggtagccg ggtttgcggc gcacagcttt
     6841 ggggcgccgc tgacgctggt catcgcgggc gcagtgtgcg tggcggcggc gggcatgttt
     6901 ctggctcaac tgccggaact gcgtaaggcg gtgcggccga tttatgtgcg caagggaatc
     6961 atgccggatg catcgcaggg cttgtcgggc gctggcgcct cggcagggat gggcccggct
     7021 gatgatcgaa gtcgcggggg cgaaccggcc acttgagcgg cgcaaggacg aacaaatcgg
     7081 ccgtcagcaa aggggtgagc gggcgggttg cgccagggcg ggttgaagcc gggtttgtgg
     7141 tcgcgatggt gctgccgctc gcggtggcgg tgctgatcgt caacctgccc gcgattcatg
     7201 gtctgggctg gccgcgttac gtgcttaatt gcctcatctg gcttacgacg ctggcaatat
     7261 tggtgcacga tcctcaaatg cgcgggcggc gcgaggattc ggcagacgcc gcagaagcgc
     7321 gccggcggga gcgcaagccg ctgctgaagc tgcgcagcga ccggacgata ctggcggcgc
     7381 ttggcgtagt ggcactgctt tcgctgctgt cgtattacga ttttggccgt tttcacggtg
     7441 gcgggcgctt cgttcattat cacgatctgt atcactacta catgggcagc aagtactttc
     7501 ccgaggtggg ttatgaggga ttgtacgccg ccacgcaccg ggctctggtg gagaacgagc
     7561 ccgcgatgtc tggcgggatc agcctggtca agaatctgcg cacatacgac atagagaacg
     7621 cccggatttc ctggcagcgg tccgatgggg ttacggcgcg gttctctgat tcgcgctggg
     7681 agcagttcaa gcacgacgtt cggttcttcc aggcgcggct ggcgccggcc gagtggcagg
     7741 ttctgctggt cgatcacggg ttcaacgcca cgcctttctg gacgggcgtc ggggcggcgc
     7801 tgtcgtcccg gctgccgctg aatagcgcga cgctgctctt gctggcgctg atcgatccgc
     7861 tgctcgtggc ggcggcgtta gtgttggtgt ggctcacgtt cgacgcgcgg acgggtttac
     7921 tggcggtcat ctttctgtgt gccaacttct ttggcgtttt tgatattacc ggcggcgcgt
     7981 tcctgcggca gctctggctc gcggcactga ttggttttgt ctgctgctat cgccgcggct
     8041 ggatgtggcc ggcggcggcg ctgttggccg tggctgcgct ggaccgggtt ttcccggccg
     8101 tctttgcgct gccggcggcc gttttgttga tcattgacac agtgcggcgg cgcagcgcgg
     8161 gccgcgttca ggcccgtttt ctcgcggcgt ttgttgcctt cgcagcgggg ctggggttga
     8221 cggtctgcct caccgttggc ggcccgcgga gctggacgga ttggtatgac aagatcagcg
     8281 cccacaatcg ctggttctac accaatcaac tttcgctacg taatttgttt accgtaaacc
     8341 cggcccagtc actgcggacc atttcggaag gctggaacga gggcctctgg ttgcgcgaac
     8401 gggagacgct cgacgcacgc acccgcaata cgctcctggc cggccggacg gtactgctgg
     8461 gtctgctgat cgcgactgtg gcgaagcgca aagatgctct ggcttctctg gccttggtgt
     8521 cgttcgtgcc attggtgctg ttttatccgg ccgactatta ctgtgttttc ctggttgccg
     8581 ccatactctg ctggcgcggc aatcgcggac tggctttggc actggtgggg ctccaagcgc
     8641 tcttttggct agtcaacata ccgctctcca gcggcgtgtg cctcgaatta ctacattggg
     8701 tgatatctct gtgcctgatt gtggtctttg tggtctttct ggggcgcgag ctgcttaatg
     8761 ggggtcaaga tcagcgggcg cggcgtaagg caccgttgcc gctgctggcc ggggcggcgg
     8821 cgattctgct ggccggcttc gtggccgata cgcgcgccgc ccgccggcag ccgctgccgt
     8881 tcgagttgga cctgacgccg gacgacgtgt tgctggcgga gaatttgcag ccgggcctgg
     8941 agcacatggc ttcctggggc aacgcctggt cgcggaacga tcacttgcta tgcgtgacga
     9001 cgggggcggc ggcccgggct gtggtgcgtg ttcccgcccc cagtgacggt atgtatagtc
     9061 ttgcggtcac atactcgacc ggcccgccct tcggaattgt gacattggca ataaacggag
     9121 aacagcccat ctcgacgttg aatctgtacg cgccgaatgt cggtgtgagg acagaggtat
     9181 acgacgaggt ggcgttgaag gggggtgata acgagttcag cttcgctgtt acgggcaagg
     9241 accgggccgc caaagaccag ttcttcgccg ttgatactat aaagctcgac tacgcggccc
     9301 cgcgcgaagc gagctccagt gctgcggcgc gccgtgcggc gctcgagcgc gcggtggcgt
     9361 ggcttatggc ccatccggcg gacagcttcg atggcgggcg tccggctata tgcaaggaaa
     9421 ttgctgtcct gcattcggtg ctgtcaacgc cgcagctcgc tgagtggggc tcgaagtaca
     9481 tgccagaact gcagcagcgc atcgagcgat tgaacaccca ctgcggcccg cgcctccacc
     9541 tgcatgaata cgacggcatg ctgctcgcgg gccacgtggc gcggcaattc aacctggaat
     9601 tcattgcact tgcaccagtt gcggaacagt tgaaaccctg gattgaatcc gtctatgccg
     9661 atcgctcggc ggcccagctc ttgtttgcgg gcgagtactt caatcgcctc tatcctgaac
     9721 aagctgtggc aatcgatgtc gaacaaagcc tcgtttaccg cgaatacacc gaacgcaagc
     9781 tcgcgcaagt gttggccggc gacgtggacc ggaacaagac ccgagccgta atggcggcca
     9841 tctcggctct cagccaggac gtctgcggcc tgaccgaatt cggccgtttg gcccctccgg
     9901 ccggatatct ggccgatcag gaattctggg ggcgcttatg cgaggacggg ctgcgctgga
     9961 gtatgcagac cggcgacctg ctcacgctgg cccgggtcgt ccaactggcc gaatgcctgc
    10021 gcgtggccgg cgcggttccc gggctgccgg ccgcggtcga tctgctggtc gagcatcagc
    10081 gagccgacgg cagcttcggc gcaattacgt gtgacaaccc caatccgtac cgcgaaggcg
    10141 tacttaacgc ggccctggcg ctgaaggcca gcctgcgctg aatcggcgcg gggtggcaag
    10201 gtggtcgcgg agcgacggcg cagagtaggc gctatctgag cgccggcggc gctacttttg
    10261 acggtaaacg attcggccgc gcttcaggtc gtagggtgag acctcgacgg tcacgcggtc
    10321 ccccggcagg atgcggatga agtgcttacg catgcgcccg gacacgtggg ccagcacttc
    10381 gtgtttcgtg ttgcccacat cggcctcgac gaggaacatc gcgttgggca acgcctttac
    10441 aacagtggct tccatctgaa tggcatcggc cttagccaat caaattcctt tcaagcaaac
    10501 ctgggcgcgg ctgccccaga aaagatggca atcctacaag ggctgagcgg cggcgtcaat
    10561 cactgttcgg aattagcgca gatgcagcgg aacgagcggg cccctaacat tactcttatt
    10621 tgcgtggaag caacgtagct gccgcccatc cggccacgcg gcagttctcg cgagcggagc
    10681 cgcacaggct ggcaggtgcg ataacgttgc gctcaccaag catcaggggt ggcaaagcgg
    10741 ccgtcgggtg gccgtcgggt gccatgcccg ggctgtaagc gcgggcatgc ccacgtgagt
    10801 cgcctcgatc atgccacacg tccggactac ggcggcgatg aatcgattga cacgttcgcg
    10861 gcggtgcacg cactgatcag ccgcgctagt atcgctgaga ctcgtgaagg gtttctgcat
    10921 tggccgttag cgggaaacga tgctctttca atggtgcgtc tcgagaatgt gaggaaacaa
    10981 tgtcgccgac gaaaactgct ggcgtgatca acggggtgga tcaggacagg ctgcaacaga
    11041 cggtgtctca gatgcggcgc gacccgggat cggccgcgtt ccagttccgg gtgcgcaaca
    11101 cctggctggg cggggcgcac aatcgctcgc ggatacagga tttttacggc ggcggaaaag
    11161 aagacgacac gcgccagaag acgtacgtca tggattgtga cgaaccgccg atcctactgg
    11221 gcaaagacca gggccccaac ccggtggagc acctgctcaa cgcgctggcg gggtgtgtaa
    11281 caaccgcact ggtgatgcac gccgcggcgc gcggcatcga gatccaatcc gtcacgtccg
    11341 aactggaggg ggacatcgac gtgcgcggct tcctggggct gtcgccggac gtgccgcgcg
    11401 gtttcaagaa catccgcgtg acaatgcacg tgcagtctga tgctccgccc gagaagctgc
    11461 gcgagttggc ggagtactcg ccggttttca acaccattgc gaatgcgaca ccggtcgaga
    11521 tcagcatcga caacgcccgc acgtagtggc gaacgcgcgc cccgccaaga ccggccagct
    11581 cttgcagcgg ccgcaaatcg gagattgagg ggagcgaatg tctggtgcaa tccgaggcat
    11641 gattgcgggt ggtccgcaaa gcttcagcgg cgtttcatgg cgcgatccat ttcgcgctcg
    11701 tgttcgcgct tcttcaggtc ggcgcgtttg tcctcgtgcg tcttgccgcg gaccaacgcc
    11761 agctcaactt tcgcaatgcc acggtccgag aaatagattg cgagcgggac gatcgtgagc
    11821 cctcgctgag tgacctttgc ctcccacttt cgaatctcgc ggcggtgcaa gaggaggcgg
    11881 cgctcgcgcg tgggggcgtg ctgatcgtaa gtggccatgg gataaggaga tatgttgcaa
    11941 tctcgcagga aaacgtcgcc ctcgcgaatc atggcgaagg cttcgtcgag cgaggctttc
    12001 ccgtcacgta agctcttgac ttcactaccg gttagagcaa ttccggcctc aagtttctcc
    12061 agaatctcgt aattaaagcg ggctttttta ttccggataa cgggagcttt gggtggacct
    12121 ttcgccatgg ctgggctaga ttgtagggag aggagtttct gctgcaaccg gcagcacccg
    12181 cctagctata aggcagtgaa agcagtggct gaagcccaaa gcggcaacca aaagaaggtt
    12241 gcgatgtcgc cggacgaact gaagcgcatt atcgcgcgag cccgcgaagg gcacgccgat
    12301 gcgttccgcg acctagtgga ggcgtacaag gaacgcttgt tcgccttcgt atggcgcatg
    12361 gttcgtgatc atcatgaggc ggaggacatc tgccaggcag ttttcgtgaa ggcttacgaa
    12421 tcgctggaca gctattcgga acaatacgcg ttcagcactt ggctcttcac gatcgcctac
    12481 cgcatgagcc tcaacagcct gcggaagaag aaggccttat cgggcgaagt cgatttcgcc
    12541 cgcttcggct gcgacgagca ggatgcctcc gcgacgctgg ccaccagcga ggaagcccga
    12601 cggctgcgaa agctgatctg ggccgcggtt gaagagttat cgaccccgca gaagtcgtcg
    12661 gtattgctct tctaccgtga gggcaagagc tgtcacgata tcggcaaggt gcttggcatg
    12721 ccggcggtaa cggtcaagag ccatctacac cgggcgcgcg aacggctacg aaaagcgttg
    12781 gcagccgaac tggtggacga gcggctggac ggtgaatacc tgagcggcag ccaaatgtta
    12841 agagcccagc gatgaattgc gcagaattcg aacgactgat cgacgcgtat ctggacggcg
    12901 aattggccgg ctccctgcga gtcgaattcg acgcccatcg cctgcggtgc cggcgctgcc
    12961 agctcaccat gatcgccatg gagtcggtcg aaacggtgct ggccaacgac cggccgctcg
    13021 caacgctgag cgatgatttc acagagcgcg tgatgggggc tatccagagc cggcgcccgg
    13081 ttgcatcacg tttgcggccg cggcgcatcg tgctcgcggc gggcctgttg ctccaggcgg
    13141 cggccctcat gttcctggct atccatccgt ggcacgaggg cggttcggtc agcgagttcg
    13201 agccggtccc ggtaacgccc gctgaaccag agggggtgcg cgatctcgac caggtttatg
    13261 cgctggtgga caagaagacc gaggagcgac aggcgctagt ggaccgcatt ttcgccaagc
    13321 tcgaggcggc cggcatgaac gttgcgaccg acctgaccca gctcgtgagt tacgcctcgg
    13381 cgctgcctgt gccggacgac gtagcgcgcg cctcggatgg aatgcatggg gcgaatccgc
    13441 tgaatctgct gatcgacgtg tttattcccg gcgctgcccc agaactcgag agcactccga
    13501 ttgggacagt cgagcacgct ctttaggctc gccggcctag tgagttgaag cgcaggattt
    13561 gataccaatc agccgcggca tccttgttgt tgcagtcttt ctcacttcca cgccgtttac
    13621 cctgactggc ttggcagcgc ccgaacgcga ttcgctggcg cattccgcac ccgcggacgt
    13681 ggggctctac atcgaggggc gcggcgtcgc tgacctgctg cagccgctga tcgagccgca
    13741 ggcctggctg gcgctggccg aattggcggg gcagccggca cggctcgagg aagcgaacgc
    13801 ctggcgcgtt tggattcgcc aggggctgcg gatggaaccg gccgaagcgt gccgggagtt
    13861 gctgggtgag cagttcgctt ttgtaggtga aggactgggg cgggctcaag acggggtggt
    13921 gatctgccgc ccggacagtg aaccggaggt tttgttgcgg cggtgggaag cgcgccctct
    13981 gccggacgcc ggccgcagct cgatttatcg cttggccaac ggcctcggcg ccgggctctg
    14041 cgacggcctg ctgatcttcg gggcggcgga catccccgac ggcatgtttt cgcgtgtgat
    14101 tgcagtcggc gaagggccgc aggggcctgc cagcctggcc gaggacgccg tgtaccgagg
    14161 tttgctggcg cgtgtgccgg aagggccgga cgctattctg ttcgcgcgtt tgcgcagctc
    14221 gacaccgggc gcggcttcac agccagccat ggcgcaagcg gcggaacgcg acgcgggccg
    14281 ttccgcttcg accagcaaac cgagcgcctg cggcgcggcc ttgcccggcc cattgcacgg
    14341 cgcgtcgaat atcttgctgg cgctgcaccg tgagcggcat ttgctgcgtt tcagtgtggt
    14401 cggcgatgcc ccggcgcgct gcgacgggac gcagagcggt ctgcccgaac tggtcggcta
    14461 tttgccggag cggacgctgg cggcctgggg gtttcatctg gattatcgaa atctgccggg
    14521 aatgctggcg cgcctgccgg agcgcagcat tctgcgtctg acgttttcga tgcaggagcg
    14581 gtcgggactt atagcacggc tgagcgaagc gcttgattcg gcgacgtgcc tggcagtcgg
    14641 cgtggtggag ccgccatcgc gcacggagcc ggcgccgccg gtgcctgctc tggcggtgct
    14701 ggtcaatgcc gtccagccgg aggaggcgga ggacgagttc gagacactgc tgaagcagag
    14761 catttcgttc tatgggttgc tggccctgaa gaccggtgcg caggtcattg tgccggacgt
    14821 gcacagactc gaggtcgaag gcgctgatgt gggtttgctg gacgtgagcg ggctgctcgc
    14881 cggccggccg ggtggggcgg cgattgctga gttgcacata tgctgggcgc tggacggtgg
    14941 gacgctgatc gtggcttcgc acctggattg gctgcggcag attttggcag cccggcatga
    15001 agtggcgccc ggcctgcaga aaactgtggc tctcagcacg cgcccggtcc cgcccggaac
    15061 ggagaccatc atcgctctgc aaacgggacc gctggcggat ctcggccaac tgtggctgga
    15121 tcacttccag aagaccgcgc cgcaaattct gagcgaaacc tggtggcagg cgcagcggcc
    15181 cggccgcggc aacgtgcatc tcgggctgac cgtgacggag gcctcgaaag agcagcggtt
    15241 ggttgtcacc gcagttaagc ccgacacgcc cgccgacggc ctgttgcggc cgggcgatca
    15301 aatcgtgggt tgtggcgggg atcagtcgcg gccgaacgcg ccgcggcgtt ttgcgacttc
    15361 tcaaccgatc cgcgagattc gcgatgcgat tgcgcgccgg cccgacgctc gctgggtgga
    15421 cttgctgatc gagcgcggcg ggacgccgct ggtgaagaga ataccattgc cgtacttcga
    15481 tccgattcag gttctgcggc ggttcatcgc actcgggcag atattccagc gtgtcgtgta
    15541 ttacgaggat gtgcccgaaa acccggtcct ctccgacccg gttctctccg cagaagtgtc
    15601 gatacaaacg gcggccgcag aggcggcgac gcaacccgcg atcacgtcct cagagccgcc
    15661 cggcggacgc ggtttcctga cgatcgaact gcgcggcagc cgcgagccgc tgttcccgtt
    15721 cgcgttaacg ccggcgcccg tgtctgcgcc gatcacagat caagcgcgca aactgccgta
    15781 acgccgccgg cctgttgaca acaaagctga ctagctaggc cgcccgcagt ggcgcgcggg
    15841 cattgcttat gatgcgactg gcaattgccg ccggcacaca aggtgcgaaa tgaataggat
    15901 tgttgcagcg gtggtggggg cctcgtgtct cctcgggaca tctacatggg gctcatgcga
    15961 cccgacgggc gactacgcgg tgccgctcgg cagctattgg cacatccgcc cggatcccga
    16021 cggcgggttt atcctgggcg gctctgcggt gtcgtggaat atggggttga cgggagtcgt
    16081 tgagctctgg gcgggccgtc agatcgtcgc aggcaggacg cgagacgctt atttcgtgct
    16141 ttttcccagc tacgaactgt tgttcggcga ggacgggcgg ctcaagaagg accctgctcc
    16201 tgtgttgggc aagctggagt tgttcgaaga gcgggaggcg tgggagcggc ggttgtcaga
    16261 gatggattgc accgacgtcg tgctgcgcgc gccgcggcgc tgggactcgc cggccgtgcg
    16321 attcgtcgtt tatgccgcca tcggcggagc atgcgccgcc gcgccgctga gcgtcttgct
    16381 cggtgctggt gcggcgcgga tgtattacga atggagagcg cgacggaatg cgagtgcccg
    16441 cgaagca
//
LOCUS       DAOSYS010000053        16317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_2954_length_16317_cov_4.993974, whole genome shotgun sequence.
ACCESSION   DAOSYS010000053 DAOSYS010000000
VERSION     DAOSYS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 16317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16317
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2954_length_16317_cov_4.993974"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..3403)
                     /locus_tag="PLU99_05000"
     CDS             complement(338..3403)
                     /locus_tag="PLU99_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOO16449.1"
                     /translation="MDEGRQRAVWWVIGVAALLSLAKPVSSADIENELAALRAQGEAE
                     GWTFTVGQNPASDRPLEELCGLVVPENWEAGARFVELSPLRLGLPARFDWREQGGCTP
                     VRNQGGCGSCWAFATVAPLECNILIRDGISVDLSEQWLLSCNRSNYSCSGGWFAHEYH
                     EWRTDSCGGSGAVLESAFPYVASRVPCNCPYAHPYTIAGWAYVGSSGGVPSTAAIKQA
                     IIDYGPVSVGVYVDSAFTNYSGGVFNACTDGTPNHAVALVGWDDADGAWILRNSWGPY
                     WGEGGYMRIQYGCSRVGYAACFVEYTGVGSPEIEVTPASRDFGDVAVGATADRVFTVR
                     NVGGDVLEGAVAAPAAPFSIVGSRGYELGPGESQSITVRFAPTMQGDFSATLQFSGGG
                     GATATVSGTGIGAGPGDDCATAPLIGDGTFTASNAAATTDGGASCAASGADVWWRYVA
                     AYPGTATIDTCGSSFDTVLSVYSGCAGAELACSDDATCDDAPGTVSQVQFSITAGTQY
                     LIRVAGKAGAVGDIMLNVDSVATTLTVSGRVTTAAGAPVAGVTLTGLPGPAVTDEDGY
                     YTAEVDYGFSGTVRPQKTAFTFSPPSITYSSLTNDRLNENYIAIPPTFVISGRIADED
                     GAGVPGVTMLGLPGNPVTSGNGQYSAVVPYGFSGTATPTKAGCRFEPAAREYQDVFTN
                     MPLEDYDAEVWNGALTVVLEPSAARLTGAKWRVDGGAWQDSGRIVADLAVGRHTVSYA
                     PAAGWSAPPPESVQIVRDGVTRVEREYLQQRYRLTVQASADLGEVIASPAPDEWGTYV
                     QDTVVTLTAVPAEGYTVQSWYGADDSPPFGTLTNTVTMTSDRTVSVVFAIDPFAIYQL
                     AVWVRGSGGTVSPRRGTYREGTVVPLLATPDDGYQVKAWAGTADDSSTANSNSVIMDG
                     SKTVYVEFEPWLDCNADGQSDRDNIASGDSADCNANGVPDECELDSDDDGLIDDCDLS
                     SADDLPIIAPQPACGFGALQAVGLMLAGLPLVGRSYRFIRHGRKDGLGR"
     gene            3631..5796
                     /locus_tag="PLU99_05005"
     CDS             3631..5796
                     /locus_tag="PLU99_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOO16450.1"
                     /translation="MTGKRLTIGFAVLSSFLVTAFAQDSRPARQRIEYPQARQTEDVN
                     QLGSLTIPDPYRWMEDLDSPELADWVAAENKITNAFLEQVPAREAIRQRLTHLWDFER
                     YGVPSQRDGRYFFAKNDGLQNQSVFYWMDGLDGTPRVLLDPNTLSPDGTIAVAGMAVS
                     HDGQELAYGLSTAGSDWVTYRVRNVASGEDRPDVLEWIKFSGVSWTKDNAGFYYSRFD
                     QPEGNVLEAVNYYNKLFYHRLGTPQSEDRLVYERPDEKEWSFGPHVTDDGRYLIISIS
                     RGTDPRNRVYYQDLQAADAPIVPLLNDFDAQYNFIDNDGPVFWFTTNLDAPRNRVVAI
                     DTRRPQREHWKEVIPQAEETLQGVNIVHDTFIASYLKDAHAQVRMFDLTGRLLRELEL
                     PGIGSAYGFGGKRSDTETFYAFTSFAVPTTIYRYDMLTQTSSVFRRPQVDFNPADYQT
                     QQIFYTSKDGTRVPMFIAHRKGIALNGDNPTYLYGYGGFNVSLTPAFSVSRIVLMEMG
                     AVVAVANLRGGGEYGQEWHDAGRLHNKQNVFDDFIAAAEWLIANKYTRPQKLAIAGGS
                     NGGLLVGACMTQRPELFGAALPAVGVMDMLRFDKFTIGWAWTSDYGSPENPDDLKYLL
                     TYSPLHNIRPGICYPPTLATTADHDDRVMPAHTFKFIAALQAAQQSAPDCDNPILVRI
                     ETKAGHGGGKPTSKIIEEVTDQWAFLARVLGMRTSQAID"
     gene            complement(5840..6589)
                     /locus_tag="PLU99_05010"
     CDS             complement(5840..6589)
                     /locus_tag="PLU99_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOO16451.1"
                     /translation="MLDQIRIVLVSPSGPANVGATARIMANMGLSDLAIVAPRCDAKG
                     EQAVAYAAHGRALLDGAAIVDDLPAALAGCTCTFATSSKLGLYRRQTAIEPVRAADEA
                     LQVARSGRVAFAFGRENFGLRTEELLNFDRIVTIPADEGYPVLNLAASVTIMCYELRR
                     AWLAAEQLPALPMALHSGFATHERKELLFKHLFEALDQIGFFFGPKPDHLKYALRHLL
                     GRVDLSVNEADILIGMARQIQWYVRHHPQRI"
     gene            7004..8374
                     /locus_tag="PLU99_05015"
     CDS             7004..8374
                     /locus_tag="PLU99_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOO16452.1"
                     /translation="MIKFGPGNLRIPKFERPVYVVAGGLTDFRKRYPEKNTCQLCNEA
                     LRMAVEENDLKVDPEQYRRMVNWCVYSQFADHFGDQLLAAAKIHDYLGFDPLGNIEVK
                     TGGATGGSSVLAAAQAIASGYADCVPVVGWERMDEVTTKVGNSYIASAACKDFESELG
                     WMYAAYYALMAKRYQYENNVPRETLAKIACKNHEYAYYSPFSQNPGKYTVKEVLENEI
                     VSDPLSYLECCVMSVGAAVLLLADEKTAYKLTDTPIRLAAICGGSHTLRTADRRHMPI
                     LLLPNENESTYKDYYGVRRKEWPGFSSFLAARMAAYLAYNMAGIKDPVEDFDVLETHD
                     AFTISDVQTYEDIGLRPYGRGREFIESGDAYFGGRLPTNLSGGLIGTMHAVGATGVFQ
                     LVEILWQLQGKYDKFHGDPKLWQRYGKTKPADWRSLQVKNPKRGCAISHAGTGSHVTC
                     AILERP"
     gene            8486..8959
                     /locus_tag="PLU99_05020"
     CDS             8486..8959
                     /locus_tag="PLU99_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOO16453.1"
                     /translation="MATDWPTSRLEVLNEEPLVVKNPKTWFHIHSYGGWGKFFKGLRE
                     KKLLATRCTNPKCEEKRLWLPPRTECPDCWHDMEWVEAPQQGTIYTFSTVKYPGELFK
                     LPAGTHLISVELPGVCTKLMSWLKEGEPQFGMKIKAVFNTERPTNTILDLAWVPA"
     gene            complement(9138..11888)
                     /gene="acnA"
                     /locus_tag="PLU99_05025"
     CDS             complement(9138..11888)
                     /gene="acnA"
                     /locus_tag="PLU99_05025"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOO16454.1"
                     /translation="MTGNRDLFGARAGLRTRGGQVEIYRLSALERAGFGQLQHLPYSI
                     RILLESVLRNLDGLATLESHVRALAHWTPASTGQHDIPFMPARVVLQDFTGVPALVDL
                     AAMRSAMDRAGGDPRQINPQIPADLVIDHSVQVDYFSRADALALNAKLEFERNRERYE
                     LLRWGQRAFDNFRVVPPATGIVHQVNLEYLAKVVLQREQDGQVTAFPDTLVGTDSHTT
                     MINGLGVLGWGVGGIEAEAAMLGQPMFMLLPKVVGFRLKGELPPGTTATDLVLRVTEM
                     LRRFGVVGKFVEFFGPGLSNMPIADRATIGNMAPEYGATVGFFPVDDQTLSYMRFTGR
                     SEDEVDLVERYCKEQGLFRTATSPEPRYSQLLELDLSTVEPSLAGPRRPQDRIALSAM
                     KSGWRECLASVFGKDGSTAASEAPRVSMVGQEFSLGHGAVVIAAVTSCTNTSNPALML
                     AAGLLAKKAVERGLRVPPWVKTSLAPGSRVVTDYYAKGGLTQYLDMLGFQTVGYGCTT
                     CIGNSGPLPEEIANAVTAGDLVVASVLSGNRNFEARIHPLVRANYLASPPLVIAYALA
                     GTVDIDLTRDPLGHDGDGKPVYLRDIWPSEQEIQDAVAQFVTPEMFKQRYADVFSGDN
                     QWRGLRIPDSQVYEWREDSTYIQEPPFFADFSPQPPRVQPLERARCLVLLGDSVTTDH
                     ISPAGSISPKSPAGRYLLEHGVRVEEFNSYGSRRGNDRVMTRGTFANVRLKNLLVLGT
                     EGGVTVHLPSGQQMSIYDAAMRYREEGTPLLVLAGADYGMGSSRDWAAKGTYLLGVRA
                     VIATSFERIHRTNLVCMGVLPLEFQPGETHQSLGLTGHEVFDIPGLAETLKPQQRLTV
                     KAVHPTTGRRTEFATICRIDTPVEMEYYRNGGVLHMVLRRMLAQSGAAAHPAPTVTAQ
                     HV"
     gene            complement(11977..12807)
                     /locus_tag="PLU99_05030"
     CDS             complement(11977..12807)
                     /locus_tag="PLU99_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16455.1"
                     /translation="MVDQLKAMLGREHGLDDVNLALRDYISRLSPPAVGALHVTCADE
                     SEFECVESFQRWIVEPLLPNLHFTEKAPFRLANLGGRYEFGAVPVAEAHFATPESRQS
                     FKVMIVKISAHVAVSGFGAATRFGEMHRYGTASHACGALHKLLDGANGAFIDDLRAAF
                     CAGEKDRLQVLLDEKQVSPAQRAFLAAIVNALLQAARAVQDIKNHAPASPTLYLVPAC
                     VTLNRPERDTEFFCGLNVCDCRTPDFRVNYYGLGDNPAQYRLACEPLGLQLTDDDGLL
                     "
     gene            13149..14312
                     /locus_tag="PLU99_05035"
     CDS             13149..14312
                     /locus_tag="PLU99_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOO16456.1"
                     /translation="MVGAPFTPDPDASVRRPRYITRLEKLTQIPPDERERLTPVVQRY
                     AMRLNDYYLSLIDWTNPDDPIRRIVIPQVEELEPWGDLDASHEADYTVAPGTQHKYSH
                     TALLLCNETCAGYCRYCFRKRLFMNDQHEARTDVRPGLRYIAEHPEITNVLLTGGDPL
                     LLSTRRLREIIAALRDIPHVQIVRIGSKIPAFDPGRILNDPELLTVLSTFSTPEKRIY
                     LMTHFDHPRELTTSAIRAIDALLRNGVICANQCPLIRGVNDDSNVLATLFRRLSWIGC
                     PPYYLFQGRPTAGNAPYRVPLVEAWEIFRATVRQGSGLAQRARFVMSHHTGKIEVVGV
                     DEQRIYMRYHRAVRLADRGRFLIYLRNDNAYWLDELTPVGNHGALPAGDGAGG"
     gene            14418..14870
                     /locus_tag="PLU99_05040"
     CDS             14418..14870
                     /locus_tag="PLU99_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOO16457.1"
                     /translation="MNPHDLANWIRQERARVDELSYLVRKHLAVPPPTGRARWIPEVR
                     ECFRRLSDHMRRHMALEESEGYMNEVRKLRPALSRELDRLQHEHGELCRLMDHIQVGL
                     DEMQPEDNLIVRSYCAQIGMLLSYVERHEEEEQNLVVYAFSEDIPEQD"
     gene            complement(14989..15063)
                     /locus_tag="PLU99_05045"
     tRNA            complement(14989..15063)
                     /locus_tag="PLU99_05045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15026..15028),aa:Pro,seq:cgg)
     gene            complement(15270..16289)
                     /locus_tag="PLU99_05050"
     CDS             complement(15270..16289)
                     /locus_tag="PLU99_05050"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOO16458.1"
                     /translation="MSLEQNYDNMTAWLKDCRRPLLVSHRRPDGDALGALAGMALALQ
                     QLGLQPQVALYEPFPKRYALLRDLVPWRLWPEASEVRASADALVILDTSAYQQLEPIA
                     EFLPQAPRMLVLDHHTTTDAIGTRPGDMRVIDSEACAVCLLIAEWVRRAGIAVTEPLA
                     TALFTGIVTDCGWFRFPCTDARTLHVAGDLVAAGAQPSRINAEIYEQDESSKLRLIGR
                     MLQSLQLHANDRLAVMYIRQADFAAVGADYSVTEDLVNEASRLGCTEATLLFTEEAGG
                     TIRVNLRSKRSLDVSALAAKFGGGGHKRAAGARLHGAWDVEVPRMIAAAIAELETPAL
                     QGGPD"
BASE COUNT         3100 a         5402 c         4942 g         2873 t
ORIGIN      
        1 ggatgaacct cctgcccgcc gacctggtac tccggatagt atggccccgg ttccgcggcg
       61 ccctcctccc gctctggagg gcgtgggccg taatcccagc cagttttttc ccaatacgcc
      121 atctgagagc tcccgatcca caggcttcgc cgcaacgatg attcttcatc gcgctccttg
      181 cagaggaatg acagagacat gagacggctt taaagatcca gggatctagg gatcgaggga
      241 tcaagggatc gagagggctc aagggtgggt gccatgccca agcgctaagc gcgggcatgc
      301 ctccccggag agaccatgta acgcggcctt tcttgggcta cctccccagg ccgtccttcc
      361 gcccgtggcg gataaacctg tacgagcggc cgaccagcgg caaccccgcc agcatcaatc
      421 cgaccgcctg cagtgcgccg aagccgcacg ccggctgcgg cgcaatgatc ggaagatcgt
      481 ccgccgacga caaatcgcag tcgtcaatca aaccatcatc gtcgctgtcc agttcacact
      541 catccggtac accattggca ttgcagtcag cgctatcgcc gctcgcaatg ttgtcgcggt
      601 ccgactggcc gtccgcattg cagtccagcc aaggctcgaa ctccacatag acggttttgc
      661 tcccgtccat tatgacacta ttcgagttcg ccgtagatga atcgtcagcc gtacccgccc
      721 aggccttcac ctggtagccg tcgtcgggcg tcgccagcaa aggcactacg gtcccttccc
      781 ggtacgttcc gcgtctgggc gacaccgttc cgccgctccc gcgcacccag accgcgagct
      841 ggtaaatcgc gaacggatcg atggcaaaga caaccgacac cgtgcggtcg ctcgtcattg
      901 tgacggtatt cgttaaagtc ccaaaaggcg gcgaatcgtc cgcgccatac cagctctgga
      961 ctgtatatcc ctccgccggc accgccgtga gcgtgaccac cgtatcctgc acgtaggtgc
     1021 cccattcgtc cggcgccggg gaggcgatta cttcgcccaa atctgcggag gcttgcaccg
     1081 ttagtcggta gcgctgctgc aagtactcgc gttccactcg cgtaacaccg tcgcgtacga
     1141 tctgcaccga ctccggcggc ggggcgctcc agccggctgc gggtgcataa ctgaccgtgt
     1201 gccgccctac cgccaggtcc gccacgatcc ggccgctgtc ctgccacgct cctccgtcca
     1261 cgcgccattt cgctcccgtc agcctggccg cactcggctc cagcacaact gttaacgccc
     1321 cgttccagac ttccgcgtcg tagtcctcaa gcggcatgtt cgtaaaaaca tcctggtact
     1381 cacgagccgc cggctcaaac ctacaaccgg ccttggtcgg tgtggccgtg ccggaaaaac
     1441 cgtatggcac aaccgcgctg tactgcccgt tgccgctcgt aaccggattg ccgggcagcc
     1501 ccagcatcgt tacaccgggc acccccgcac cgtcttcgtc ggcaatacgc ccggaaatca
     1561 cgaatgtcgg tgggatcgca atgtagtttt cattgagccg gtcgttggtc aacgaactat
     1621 aagtgatgct cggtggcgaa aacgtgaatg cagtcttctg cggccgcacc gtcccgctga
     1681 agccatagtc tacctctgcg gtgtagtatc cgtcttcgtc agtcacggcc ggccctggca
     1741 gtcccgtcag cgtcacgccc gcgacgggcg cgccggcggc ggtggtcacg cggccgctga
     1801 cggtcaaggt cgtcgccacc gagtcgacgt tcagcattat gtcgccgacc gccccggcct
     1861 taccggccac gcgaatcaag tactgcgtgc cggccgttat cgaaaactgt acctgcgaaa
     1921 ccgtcccggg cgcgtcgtcg caggtcgcat cgtcgctgca cgccagttcc gcgcctgcgc
     1981 agcccgaata gacgctcaat accgtatcga agctgctgcc acacgtgtca atcgtggccg
     2041 tccccggata cgcggccaca tagcgccacc acacgtcggc cccgctggca gcgcacgacg
     2101 cgccgccgtc cgtagtggcc gccgcgtttg atgccgtgaa ggtgccgtcg ccgatcagcg
     2161 gcgccgtagc gcaatcgtcg cccggccccg caccgatacc cgtaccagac accgtggccg
     2221 ttgccccacc gccgccgctg aactgcagcg tggcgctgaa gtcgccctgc atcgtcggcg
     2281 cgaagcgcac cgtgatggac tgcgactcgc ccggccccag ctcgtatccg cgtgagccga
     2341 caatgctgaa cggcgccgcc ggcgccgcca cggcaccttc caacacgtcg ccgcccacgt
     2401 tcctgaccgt gaagaccctg tcggccgtcg cgcccaccgc cacgtcgccg aagtcgcggg
     2461 aagccggcgt gacttctatc tccggacttc caacgccggt gtactctacg aagcaggccg
     2521 cataacccac tctcgagcac ccatactgga tgcgcatgta accgccttcg ccccaatacg
     2581 gtccccacga gttgcgcaaa atccatgccc cgtccgcgtc gtcccagccc actagagcca
     2641 cggcatggtt gggtgtgccg tcggtgcagg cgttgaatac gcccccactg taattggtaa
     2701 acgcgctgtc tacataaacc cctaccgaaa ccggcccgta atcgatgatc gcctgcttga
     2761 tggccgccgt actcggcaca ccgccgctac tgccaacgta ggcccagccg gcgatcgtgt
     2821 acggatgcgc atacgggcaa ttgcacggca cgcgactggc cacgtaaggg aaggccgact
     2881 caagcaccgc cccgctgccg ccgcaggaat cggtgcgcca ttcatgatac tcgtgcgcga
     2941 accacccgcc ggagcagcta tagttgctgc gattacaact cagcagccac tgctcggaca
     3001 gatcgacgct gatcccatca cgaatcagga tgttgcattc cagcggggcg accgtcgcga
     3061 acgcccagca cgaaccgcac ccaccctggt tgcgaaccgg cgtgcacccg ccctgctcac
     3121 gccagtcaaa cctcgccggc aatcccaggc gtagcggcga taactcgacg aaacgtgccc
     3181 cggcctccca gttttccggc acgaccagcc cgcacaattc ctctagcggg cggtcactgg
     3241 ccggattttg gcccaccgta aacgtccaac cttccgcctc gccctgggcc cgcaaagcgg
     3301 cgagctcatt ctcgatatcc gcgctactca ccggcttcgc caggctcagc agcgctgcga
     3361 cgccgataac ccaccacaca gcccgctggc gcccctcgtc cattgtcggc ctcctcgcgc
     3421 gatagcactt tcgcgcgcag gcgcaagcgc ccgcgcgtca atgtattatg caaattgccg
     3481 gcgccacctg cataaccatt cgcgctcgcc gcgccgcgcc cgaaattgtg gggctcgtag
     3541 ttggcaattt agtgcgtccg aacaataatc cgaacgttta tgagacgatc cccaagatca
     3601 acgcagcggg cgttctcccg tgaggtattc atgaccggca aacgattgac tatcggattc
     3661 gcggttctca gttcctttct cgtgacggcg ttcgcccagg attcgcggcc cgcgcgccag
     3721 cgcatcgaat atcctcaggc acgacagacc gaggacgtca accaactggg cagcctgacc
     3781 atccccgacc cgtatcgctg gatggaggat ttggactcgc ccgaactggc cgattgggtc
     3841 gcggccgaga acaagatcac caacgccttc ctcgagcagg ttccggcccg cgaggcaatc
     3901 cgccagcgcc tgacgcacct ctgggacttc gagcgctacg gggtcccaag ccaaagggac
     3961 ggccgctatt tcttcgccaa gaacgacggc ttgcagaacc agagcgtctt ttattggatg
     4021 gacgggctcg acggcacgcc gcgcgtgctg cttgacccca acacgctctc cccggacggc
     4081 accatcgccg tggccggcat ggccgtcagc cacgacgggc aggaactggc ttacgggctg
     4141 tcgaccgccg gctcggactg ggtcacttac cgcgtgcgca acgttgccag cggcgaggac
     4201 cggcccgacg tgcttgagtg gatcaagttc agcggtgtgt cgtggaccaa ggacaacgcg
     4261 ggcttctact acagccggtt cgatcaaccc gagggcaacg tgctggaggc ggtgaattac
     4321 tacaacaagc tgttctatca ccggctggga acgccgcaat ccgaagaccg cctggtctac
     4381 gagcgcccgg atgagaagga atggagcttc ggcccgcacg tgaccgacga cggccgctat
     4441 ctgatcatct cgatctcgcg cggcaccgac ccgcgcaacc gcgtgtacta ccaggatctg
     4501 caggcagcgg acgcgccgat cgtgccattg ctgaacgatt tcgacgccca gtacaacttc
     4561 atcgacaatg acggcccggt gttctggttt acgactaacc tcgacgcgcc gcgcaaccgc
     4621 gtggttgcca tcgatacgcg ccggccgcag cgtgagcact ggaaagaggt cattccgcag
     4681 gcagaagaga cgctccaggg tgtgaacatc gtgcatgaca cgttcatcgc ctcatacctc
     4741 aaggacgctc acgcccaggt ccgcatgttc gacctcaccg gccgcctgct gcgcgaactg
     4801 gagctgcccg gaatcggcag cgcctacggc ttcggcggaa agcgcagcga taccgaaacg
     4861 ttctacgctt tcaccagttt cgccgtgccg accacgatct accgctacga catgctcacg
     4921 caaaccagct ccgtctttcg ccggccccag gtggacttca atcccgccga ctatcagacg
     4981 cagcagattt tctataccag caaagacggc acgcgcgtcc ccatgttcat cgctcatcgt
     5041 aaaggcatcg cgctcaacgg cgacaatccc acctatctct acggctacgg cggattcaac
     5101 gtttcgctca cgcccgcgtt ttccgtcagc cgcatcgtct tgatggagat gggggccgtg
     5161 gtggccgtgg ccaacctgcg cggcggcggc gaatacggcc aagagtggca tgacgccggc
     5221 cgcttgcaca acaagcagaa cgtcttcgat gacttcatcg ccgcggccga atggctgatc
     5281 gccaacaagt acactcgccc gcagaagctg gccatcgccg gcggcagcaa tggcggcctg
     5341 ctggtgggag cgtgcatgac gcaacggccg gagctgttcg gggcggccct gccggcggtg
     5401 ggcgtcatgg acatgctgcg tttcgacaag tttacgatcg gctgggcctg gacatccgac
     5461 tacggttcgc cggagaaccc ggacgacttg aagtatctat tgacttacag tccactgcac
     5521 aacattcgcc cgggcatatg ctatccgccc acgctggcca ccacggccga ccacgacgac
     5581 cgcgtcatgc cggcccatac cttcaagttc atcgcagccc tgcaagccgc gcagcaatcg
     5641 gccccggact gcgacaaccc gatcctggtg cgcattgaaa ccaaggccgg ccacggcggc
     5701 ggcaagccca cgtcaaagat catcgaggag gtcaccgacc agtgggcctt tttggcgcgc
     5761 gtgttgggga tgcgaacgtc acaggcgata gactagctgc agcgcttcgt cttctcattc
     5821 agggcgcttt cagggcaggt cagatccgct gaggatggtg ccgcacatac cactgaattt
     5881 gccgtgccat cccaattagg atgtccgcct cgttcacgct caggtccacg cgccccagca
     5941 agtgccgcag cgcgtatttg aggtggtccg gcttcgggcc gaagaaaaag ccgatctgat
     6001 ccagcgcctc gaacaagtgc ttgaacagca gttccttgcg ctcatgcgtg gcaaagccgg
     6061 agtgcaaagc catcggcagc gccggcaact gctccgccgc cagccaggcc cgccgcagtt
     6121 cgtagcacat gatcgtcacc gaggccgcca ggttcagtac cggatagccc tcgtcggctg
     6181 gaatcgtgac gatgcggtcg aagttgagca gttcctcggt acgcaggccg aagttctcgc
     6241 ggccgaacgc gaacgccacg cgcccgctgc gcgctacctg tagcgcctcg tcagccgccc
     6301 ggaccggctc gatggccgtc tgtcggcggt agagcccgag cttggaggag gttgcgaagg
     6361 tgcacgtgca gccggccagg gcggcgggca ggtcgtccac gatcgcggcc ccgtcgagca
     6421 gcgctcgccc atgggcggca taggcgaccg cctgttcgcc cttggcgtcg cagcgcgggg
     6481 ccacgatcgc cagatccgat agccccatgt ttgccatgat gcgcgcagtg gctccgacgt
     6541 tggccgggcc actcggcgaa accaatacga tgcgaatttg atcgagcata gggccaagat
     6601 tttacgcgct cgggacggcg ccggcgtcca ccgggagcga aacttgattc gcgccgggtc
     6661 gcgctggtga tcggcgagtc gcgcgaacgt ctcggtcttt gccatccggg gccatctggc
     6721 gtgcactagg tcgcccccgc gactcctgcg attttccgac cctgcgcatc cgacactcca
     6781 gcagttcgtt aactactgtg gcgcattcct ggcttgcact gcgaattcag atttcgcgcg
     6841 tcttgatgcc ggtcaatctt tgaagagctc gccccctgcc gttgacaacc ccaacccgcc
     6901 ccgtcaactt acccttctgg cccgcgagtc cggtcctaag cgcggcggcg ccatttcctc
     6961 gcgttccgtc cggacgccgg cccatctgga aaggagacgg tcagtgatca aattcggccc
     7021 cggaaatctg cgcatcccga aattcgaacg gccggtctac gtcgtcgccg gcgggctaac
     7081 ggacttccgc aaacgttatc ccgaaaagaa cacctgccaa ctttgcaacg aagccttgcg
     7141 catggcggtt gaagagaacg acctcaaggt cgatcctgag cagtaccgcc gcatggtcaa
     7201 ctggtgtgtt tacagccagt tcgccgacca cttcggcgat cagttgctcg cggcggccaa
     7261 gatccatgac tacctgggtt tcgaccctct cggcaacatc gaggtcaaga ccggcggcgc
     7321 gaccggcggc tcgtccgtac tggccgccgc ccaggccatc gcctccggct atgccgactg
     7381 cgtcccggtc gtcggctggg agcgcatgga cgaggtgacc accaaggtcg gcaactcgta
     7441 catcgccagc gccgcctgca aggactttga gagcgaattg ggctggatgt acgcagccta
     7501 ttacgccctt atggccaagc gttatcagta tgagaacaac gtgccgcgcg agacgctggc
     7561 caagatcgcc tgcaagaacc acgagtacgc ctattactcg ccgttctcgc agaacccggg
     7621 caagtacacg gtcaaggaag tgcttgagaa cgagatcgtc tccgacccgc tctcgtacct
     7681 cgaatgctgc gtcatgagcg tcggggcggc cgtgctgctc ctggcggacg agaagaccgc
     7741 gtacaagctg actgacacgc cgatccgcct ggcggccatc tgcggcggct cgcacacgtt
     7801 gcgtacggct gaccggcggc acatgcccat tctgctgctg cccaacgaga acgagtccac
     7861 ttacaaggac tactatggcg tgcggcgcaa ggaatggccc ggtttcagct cgttcctggc
     7921 cgcccgcatg gccgcgtatc tggcctacaa catggccggc atcaaggacc ccgtcgagga
     7981 cttcgacgtt ctcgaaacgc acgacgcctt cacgatcagc gacgtgcaga cgtacgagga
     8041 tatcggcctg cgcccctacg gccgcgggcg tgagttcatt gaatccggcg acgcgtactt
     8101 cggaggccgg ctgccgacga acctgtcggg cggcctgatc ggcacgatgc acgcggtcgg
     8161 cgccacgggc gtatttcagt tggttgagat tctgtggcag ctccagggca aatacgacaa
     8221 gttccacggc gacccgaagc tctggcagcg ctacggcaag accaagccgg ccgactggcg
     8281 cagtctgcaa gtgaagaacc cgaagcgcgg ctgcgcgatc tcccacgccg gcaccggcag
     8341 ccacgtgacg tgcgcgattc tggaaagacc gtagacagat taggcccgtt agcgatgtca
     8401 cgaagttgca aagtcacaaa gtcaccaggc gggcgcacga gaccgttcct ttgtgacttg
     8461 gcaacgtggt gacttgagga taatcatggc aaccgattgg ccaacttcca gactagaagt
     8521 actcaatgag gaaccgctgg tcgtcaagaa tccgaagacc tggttccata ttcacagtta
     8581 cggcggctgg ggcaagttct tcaaggggct gcgtgaaaag aagctcctcg ccacgcgttg
     8641 cacgaatccc aagtgcgagg aaaagcgcct ctggctgccg ccgcggaccg aatgcccgga
     8701 ttgctggcac gacatggaat gggtcgaagc cccgcagcag ggcacgatct acacattctc
     8761 gaccgtgaag tatcccggcg agctgttcaa gctgccggcc ggcacgcacc tgatcagcgt
     8821 tgagctgccg ggcgtgtgta cgaagctgat gagctggctc aaggaaggcg agccgcagtt
     8881 cgggatgaaa atcaaggcgg tcttcaacac cgagcggccg acgaacacga tcctcgacct
     8941 ggcttgggtg ccggcgtagc caagggtatc ttgggccagg tgccagatgt gccgggtgcc
     9001 atgcccaagc gctaaagcgc gggcatgccc gcgcttacag cttgggcatg gcacccacgt
     9061 tatccactca cgttatccac taacgttacc cacaccgcgc cacctactca ctggcaaacg
     9121 tcaggcgccg gcgcgagcta cacgtgctgt gccgtcaccg tcggcgccgg gtgtgctgcg
     9181 gcccctgact gcgccagcat gcggcgcagc accatgtgca acaccccgcc attgcggtaa
     9241 tactccatct cgaccggcgt atcgatgcgg cagattgtcg cgaactccgt tcgacggccg
     9301 gtagtggggt gaaccgcctt caccgtcaga cgctgctgcg gcttaagcgt ttcggccaga
     9361 cccgggatgt caaacacctc gtggccggtg aggcccagcg actggtgcgt ctctccgggc
     9421 tggaattcca gcggcaggac gcccatgcag accagattcg tacggtgaat gcgctcgaaa
     9481 cttgtcgcga tgaccgcccg cacgcccagc agatatgtcc cttttgcagc ccagtcgcgc
     9541 gagctgccca tgccgtaatc ggcaccggcc agcacgagca gcggcgtacc ctcctcgcga
     9601 tagcgcatgg cggcgtcgta aatgctcatt tgctgcccgc tcggcaggtg gacggtcacg
     9661 ccgccttccg tgccgaggac cagcaggttc ttcagccgca cgttggcgaa ggtgccgcgc
     9721 gtcatcacgc ggtcattgcc gcgccgcgag ccatagctgt tgaattcctc cacccgaacg
     9781 ccgtgctcca gcaggtagcg accggccggc gacttgggac tgatcgagcc cgccgggctg
     9841 atgtggtcgg tggtcacgct gtcgccgagc aagaccaggc agcgcgcccg ctccagcggc
     9901 tgcacgcgcg gcggctgggg cgagaagtcg gcgaaaaacg gcggttcctg aatgtacgta
     9961 ctgtcttcgc gccactcgta gacttgcgag tcgggaattc gcaagccgcg ccactgattg
    10021 tctccggaaa acacgtccgc gtagcgctgc ttgaacatct ccggcgtgac gaactgcgcg
    10081 acggcgtcct ggatttcctg ctcgctgggc cagatatcgc gcaagtagac cggttttccg
    10141 tccccatcat ggcccagtgg atcacgcgtc agatcaatgt cgaccgtgcc ggcgagcgcg
    10201 tacgcaatca ccagtggcgg cgaggccagg tagttggccc gcaccagtgg gtgaatgcgg
    10261 gcttcgaaat tgcggttgcc gctcaggacc gaggcaacca ccaaatcgcc cgccgtgact
    10321 gcgttggcga tctcctcggg cagcggaccg ctgttgccga tgcacgtcgt gcagccgtag
    10381 ccgaccgttt gaaaacccag catatccagg tactgcgtca ggccgccctt ggcatagtag
    10441 tcggtcacga cgcgcgagcc cggggccagg ctggtcttca cccagggcgg aacgcgcaag
    10501 ccgcgttcga ccgccttctt ggccagcaga cccgccgcca gcatcagggc gggattgctc
    10561 gtattcgtgc agcttgtgac ggccgcaatc acgaccgcgc cgtggcccaa gctgaattct
    10621 tgcccgacca tagaaacgcg cggcgcctcg ctcgcggccg tcgaaccatc cttgccgaaa
    10681 acgctggcca ggcactcgcg ccagccggac ttcatggctg acagcgcgat ccggtcctgc
    10741 ggtcgccgcg gcccggccaa actgggctcg acggtggaca aatcaagttc cagcaactgt
    10801 gagtaacgcg gctcgggact ggtggcggtg cggaaaaggc cctgttcttt gcagtagcgt
    10861 tcgaccaggt cgacctcgtc ctcactgcgc ccggtaaagc gcatgtaact caaagtctga
    10921 tcgtccaccg ggaagaaccc gaccgttgcg ccgtactccg gggccatgtt gccgatggtg
    10981 gcgcggtcgg cgatgggcat gttgctcagg ccgggcccga agaactcgac aaacttgccg
    11041 accacgccga agcggcggag catttccgtc acgcgcagca ccaggtcggt agcggtcgtg
    11101 cccggtggca actctccctt cagccgaaat ccaacgacct tcggcaacag catgaacatg
    11161 ggctggccca gcatagccgc ctcggcctcg atcccgccga ccccccaacc cagcacgccc
    11221 agaccgttga tcatggtggt atggctgtca gtgccgacca gggtgtcggg aaaagcggtg
    11281 acctggccgt cctgctcgcg ctggagcacc actttcgcca gatactcaag gttgacctgg
    11341 tgaacgatgc ccgtggccgg cggcacgacg cggaagttgt cgaacgcgcg ctgcccccag
    11401 cgcagcaatt cgtagcgctc gcggtttctt tcgaattcca attttgcgtt aagggccagc
    11461 gcgtccgcgc gcgagaaata gtcgacctgg acggaatgat cgatcaccag atctgcgggt
    11521 atctgcggat tgatttgccg cggatcgccg cccgcgcggt ccatggcgct gcgcatggcg
    11581 gccaagtcaa ccagtgccgg cacgcccgtg aaatcctgca aaacgacccg ggccggcatg
    11641 aacggaatgt cgtgctggcc ggtcgaagcc ggtgtccagt gtgccaacgc tcgaacgtga
    11701 ctctcaagcg ttgcaaggcc gtccaaattc cgcagcacgg attccagcag aatgcggatg
    11761 gaatacggca gatgctggag ttgcccgaag ccggcgcgct caagtgcact caggcgatag
    11821 atttcgactt gcccgccgcg ggttcgcagc ccggcgcgag cgccaaacag atctcgatta
    11881 ccagtcaagg tcggcctcct gtaagcaccc tacagtctgt tcaaaccaga tgataggggc
    11941 gtttgaggcg ttccgcccgt aagcggcacg acgtattcag agaagcccgt cgtcgtccgt
    12001 gagctgcaag ccgagcggtt cgcacgccag gcggtactgc gcgggattgt cgcccaagcc
    12061 gtagtagtta acgcgaaaat caggagtgcg acagtcgcaa acatttaatc cgcagaagaa
    12121 ttcggtatcg cgctccgggc gattgagcgt gacgcaggcc gggacaaggt acagggttgg
    12181 gctggccggc gcatggttct taatatcctg cactgctcgc gcggcctgga ggagagcgtt
    12241 aacgatcgct gccaaaaagg cgcgctgtgc cggactgact tgcttctcat ccagcaggac
    12301 ctggaggcgg tctttctcgc cggcgcaaaa cgcggcgcgc aggtcgtcga tgaacgcgcc
    12361 atttgcgccg tccaagagct tatgaagtgc gccgcaagcg tgagacgcag tgccgtagcg
    12421 gtgcatttcg ccgaaacgcg tcgcggcgcc gaatccgctg acggccacat gagcgctgat
    12481 cttcacgatc atgaccttga aagactgccg cgattccgga gtggcaaaat gcgcctccgc
    12541 gacgggcacg gcgccaaact cgtagcggcc gcccagattc gccaggcgaa acggggcctt
    12601 ttccgtgaag tgcaggttgg gcagcagcgg ctcgactatc cagcgttgga aagattcgac
    12661 gcattcgaat tcgctctcgt ccgcacaggt gacgtgcaac gctccgacgg ccggcgggct
    12721 aaggcgcgaa atgtagtccc gcagggcgag attgacgtca tccaggccgt gctcgcggcc
    12781 cagcatggct ttgagttggt caaccatggc ttctctcctg ggttgctagg cggggtatat
    12841 caaatttggg gctactggca agcatcggct agcgatgtgg ctggcgcgtc acgcagaata
    12901 tcaaggtcct gatgtcttga tgacgcgccg gcccggcaga tcgcgacgtc cgtgagacaa
    12961 aaaccgggca gcgagcttga acccggactt gccgccggct gtataatggg tgacggctaa
    13021 gggggtcaag ggtgcgggtt gcgcgtgtgg cccgcaggca ttttggatgg tcgggccgcc
    13081 attcttccga taacccgggg gcgcgtctgg tgcgcgaacc ccggcccggc acatggaagg
    13141 agaacgccat ggttggggct ccatttacgc cggacccgga cgcatctgtg cgacgcccac
    13201 gctatatcac ccggctcgaa aaactcacgc aaatcccgcc cgatgagcgc gagcgactga
    13261 caccggtggt acagcgctat gcgatgcggc tgaacgatta ctatttgagt ctgatcgatt
    13321 ggaccaaccc cgacgacccg atacgccgga tcgtcatccc acaagtggag gagctggagc
    13381 catggggcga cttggacgcc agccatgagg cggactacac ggtcgctccg ggcacgcagc
    13441 acaagtactc ccacaccgcg ctcctcctgt gcaacgaaac gtgcgcggga tactgccgct
    13501 attgcttccg caagcggctg ttcatgaacg accaacacga ggcgcgcacc gacgtgcggc
    13561 ctggcctgcg ttacatcgcg gagcacccgg aaatcacgaa cgtgctgctc acgggcggcg
    13621 atccgttgct gctgagcacc cgccggctgc gcgagatcat cgccgccttg cgggacatcc
    13681 cgcacgtcca gattgtccgc atcggctcca aaattccggc cttcgacccg ggccgcatct
    13741 tgaacgaccc cgagctgctc acggtcctca gcaccttctc cacgccggag aagcgaattt
    13801 acctgatgac gcatttcgat catccgcggg agctcacgac ttcggccatc cgggcgatcg
    13861 atgcgctgct gcgcaatggc gtgatctgcg ctaatcagtg cccgctgatt cgcggcgtga
    13921 atgacgattc caacgtgctg gcgacgctct tccggcgatt gtcgtggatc ggctgcccgc
    13981 cgtactacct gttccaaggg cgcccgacgg ccggcaacgc tccctatcgc gtgcccctgg
    14041 tggaagcctg ggagattttc cgcgccactg tgcggcaggg ctccggcctc gcccagcggg
    14101 cacgcttcgt tatgtcccac cacacgggca agatcgaggt tgtgggcgtc gatgagcagc
    14161 gtatttacat gcgctatcac cgcgccgtcc ggctcgcgga ccggggcagg ttcctgattt
    14221 acctcagaaa cgataacgcg tactggctgg acgaactcac gccggtcggc aatcatgggg
    14281 cgctccccgc gggcgacggg gccggcgggt gatgccggcg acgcggcttg gcgtcgagca
    14341 gcgacagggt tgttggtttg tgctgctgct gagctggcag ggcagcacgc gagcagttac
    14401 cagcgtcgga ggtacctatg aatccgcatg acctggccaa ttggattcgg caagagcggg
    14461 cccgcgtgga tgagttgtca tatctggtgc ggaagcacct tgcggtaccc ccgccgaccg
    14521 gccgggcacg ttggattccc gaagttcgcg agtgcttccg gcggctgtcg gaccacatgc
    14581 ggcggcacat ggcgctcgag gaatcggaag gctacatgaa tgaggtgcgc aagctgcggc
    14641 cggcgctgag tcgcgagctg gaccgcctgc aacatgagca cggcgaactt tgccgcctga
    14701 tggaccatat ccaagtcggg ctggatgaga tgcagccgga agataatttg atcgtgcgga
    14761 gttactgcgc ccagatcggg atgctgctgt cgtacgtgga gcggcacgag gaggaagagc
    14821 agaacctggt cgtgtacgcg ttttcggagg acattccgga acaggactga agcgacccca
    14881 cgccgtagaa ctcgacggca cccacgaata aatgcgcgag ctcgcaagcc gtgttcgcgc
    14941 aaagaaaagg ccctccgtca gcagcggagg gccgtctctt aaccccactc ggggcgactg
    15001 gatttgaacc agcgacctct tggtcccgaa ccaagcgctc tagccaggct gagctacgcc
    15061 ccgtcgccga atttccagcg gcggaatccc aaaatcatac agcttttgag cacgcgtgca
    15121 aggttgcgcc gaaacgcgaa acacgaaacg tgcagcacga gcgaaacgcg tccatctcgt
    15181 gatgaatcgg cttaaatagc ctgccggaac agggctcgcc ggacccgccg tattgctcca
    15241 gaagcctgtt ttaatcgggc cctgttctgt taatcgggcc ctccttgcag cgcgggcgtc
    15301 tccagctccg cgatggccgc cgcgatcatc cgcgggactt ccacgtccca cgccccgtgc
    15361 agccgggcgc cggcggcgcg tttgtggccg cccccgccga atttcgccgc cagagcggat
    15421 acgtccaagc tgcgcttgct ccgcagattc acgcggatcg tgccgccggc ctcctccgtg
    15481 aatagcagcg tggcttcggt gcagcccagc cgggacgctt cgttcaccag atcctcggtc
    15541 acgctgtaat cagccccaac cgccgcgaag tccgcctgac ggatgtacat caccgccagg
    15601 cggtcgttgg cgtggagctg caacgattgc aacatgcgcc cgatcagccg cagcttggac
    15661 gactcgtcct gctcatagat ttccgcgttt atgcggctgg gctgcgcgcc ggccgccacc
    15721 aggtcgccgg ccacgtgcaa cgtccgcgcg tcggtacagg gaaagcggaa ccagccgcaa
    15781 tccgtgacga ttccggtaaa cagcgcggtc gcgagcggtt cggtcaccgc tatcccggcc
    15841 cgtcgcaccc actccgcgat gagcaggcac acggcacagg cttccgaatc gatgacgcgc
    15901 atatcgccgg ggcgcgtgcc gatcgcgtcg gtcgtggtgt gatggtcgag caccagcatg
    15961 cgcggggcct gcggcagaaa ctcggcaatc ggctcaagct gctggtaggc cgacgtgtcc
    16021 agaatgacaa gggcgtccgc gctcgcacgc acttcggacg cttccggcca cagccgccat
    16081 ggcacaagat ctcgcaaaag cgcatatcgc ttggggaacg gctcgtacaa cgcaacctgc
    16141 ggctgaagcc caagctgctg cagcgccagc gccatcccgg ccaaagcgcc gagcgcgtcc
    16201 ccatccggcc ggcggtggga aaccagcagc ggccggcggc agtcctttag ccaggcggtc
    16261 atgttgtcat agttctgctc gaggctcatg attgggattg taaggagtca gagcgca
//
LOCUS       DAOSYS010000054        15974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3048_length_15974_cov_4.957975, whole genome shotgun sequence.
ACCESSION   DAOSYS010000054 DAOSYS010000000
VERSION     DAOSYS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_15974_cov_4.957975"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLU99_05055"
     CDS             complement(<1..455)
                     /locus_tag="PLU99_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16459.1"
                     /translation="MLDGLTTTTAAAPRVWIVAEAVGEPSEPWLWRQITGLRRIRPHV
                     VCWRHTNEAAYPLCNIPRTVLPFQPHPFEGTGRWMYRLRNLPNWNFFASVGSERRFLC
                     ELARRTRPQVILAHFGHFALRVEPIARQFGIPLVTHFHGMDISSMLRNK"
     gene            complement(448..1431)
                     /locus_tag="PLU99_05060"
     CDS             complement(448..1431)
                     /locus_tag="PLU99_05060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOO16460.1"
                     /translation="MALLVSILIPVYNNRRYVGAAIDSALAQTYEPLEVVVIDDGSSD
                     GSLEVAQSYAQRDPRVRVIAHSRNQGLPATRNEAVERARGEIIANLDSDDTAMPNRVA
                     RQLAYLQTHPDCIVLGGQALFIDEDGDPVGMSDQKLSHEEIEEELFEGRGLALLQTTS
                     MLRRRDVLAVGGYRAGLRTCEEHDLFLRLGERGRLANLPDVLGAMRKHPGSFTAIGRA
                     EDDNALRRKVIADALERRGLPRDSRRPRVWPAPQSLAEWHATWAGEAFYHGFRFAGWK
                     HLRRAFCSNPLCLPAARILTDAVFGFGTRARVRRALVVLGLRRPPVETEHA"
     gene            complement(1441..3024)
                     /locus_tag="PLU99_05065"
     CDS             complement(1441..3024)
                     /locus_tag="PLU99_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16461.1"
                     /translation="MSDLFNKRTVVINSASGVTAKALNVVILLWLHQHLLKRITPEEY
                     SLYPVILGLMVFPPILATLLTSGLGRYVLEAHVQGDERGVRRVVSTMLPFLLTAGLLV
                     LAGGWWAAWRIDAWLRLEPERVWDARLMLGMMILPVALSFPVSPFSCGLWVRQKFVWI
                     NSIELGGEILRAALLLVLLLGVNTRVIWVATATMTSQVIKLLVLMYASRRAVPALRWR
                     WRDFHWPTAHALTRFGFWSLIGQCADQIRAGAHPLILKNLSTAASVATFNIGWIFFHQ
                     IALLEYIVTRPLQPALTALHAAGDRERLRRAYLCGGRYGLWGTMLLVLPLLVYRTEFV
                     TLYAGANYIEAGAILGLLLLTFPLEYGNNMMARLAYASGELRPWAWRMLLVQLLTVAG
                     AVLLVGRLHMGAFGAALATLLVIAVAQPLLFWPLGLRMAGVSWREWFVATLWPGLLPG
                     VFGVVAWYAPKMVMTVDSWPSLMACGAAGAAVYCLAVLTLGLRASERRQLAAALARVR
                     QRLHAGRLDAVTETPLAPS"
     gene            complement(3021..>3575)
                     /locus_tag="PLU99_05070"
     CDS             complement(3021..>3575)
                     /locus_tag="PLU99_05070"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOO16462.1"
                     /translation="LAGEHLSLAHCPDALIVRTCGLYGHAGARGKGGNFVETMLRLAD
                     SGQKVRVVNDQRLSPTATADCAAKVVELLRVDARGIVHAAAQGACTWFEFAREIFTLS
                     GRAVDLRPISSADYAAPARRPEFSALGSRRLAALGLAPCRSWREMLADYLAARAPRPP
                     EPKEIAGVECCRTSGAAPRGTGPE"
     assembly_gap    3576..3675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3676..4523)
                     /gene="rfbB"
                     /locus_tag="PLU99_05075"
     CDS             complement(<3676..4523)
                     /gene="rfbB"
                     /locus_tag="PLU99_05075"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOO16463.1"
                     /translation="MLNRILVTGAAGFIGSNFVRRLREKRPEWQVVAFDALTYAGNLE
                     NLRGLDDSPQFEFVRGDICDSEAVRAVLDTQVGAVVNFAAETHVDRSIHGSADFIRTN
                     VLGTQVLLEQSRLSAVRRFLQVSTDEVYGSLGPEGRFTEDSPLCPNSPYSASKAAADL
                     LVRAYHRTYGLPAVVTRCSNNYGPYQFPEKVIPLFITNLLEERKVPLYGDGLNVRDWI
                     HVQDHCDALLAVLERGQPGEVYNIGAGNEMTNLELTHALLRELRHDASMIHYVKDRLA
                     HDRRYAV"
     gene            complement(4533..5294)
                     /locus_tag="PLU99_05080"
     CDS             complement(4533..5294)
                     /locus_tag="PLU99_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOO16464.1"
                     /translation="MKGVILAGGLGKRLWPLTKITNKHLLPVFDKPMIYYPIECLVNA
                     GIRDVLLVTGGNDAGDFLRLLGNGKEFGLKELHYTYQVGEGGIADALRLAEDFADGDK
                     IVVVLGDNIIERDIRRGVANFYSQPRGARIFLKEVPDPERFGVAVLEDGRVTRIVEKP
                     EVPESAYAVVGIYMYDEQVFDFISSLKPSARGELEITDVNNFYLEQGTLEYEILEGWW
                     TDAGTFESLLKAANLTAGGGANHTRPMAAAVPAAV"
     gene            complement(5331..>6845)
                     /locus_tag="PLU99_05085"
     CDS             complement(5331..>6845)
                     /locus_tag="PLU99_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO16465.1"
                     /translation="GYDFAGLLRGSNTCHLPRHLVQLQGNARGAQERVVYDSELRVRC
                     VQRLYDGVTQVQAVGIGASLISAAVARHLDLSAGLSPAQIRRQLAAHDIPSTDLTVHG
                     PTFDLSTEAGLLALNEQVVLGSTRRARRSAGRRSEEFSLWAGPGSSIDPHSRLYGPIV
                     VHAGARIAPHAVVIGPAVLGPGAQVARGSLVCQCVFGPGARVSAGDCIVRRVLVGQAP
                     AKAAAGHKPSAPASAQRSEPRLLRRRRRRAASRAPTGDKRWYPRLKRAIDFSAALVGL
                     VVLAPLLGVTALLVKLSSRGPVLFGHEREGLNGRVFRCWKFRTMIDRAHQQQRALYVK
                     NAVDGPQFKLPNDPRVTPLGKLLRATNIDELPQLYNVLLGHMSLIGPRPSPFRENQIC
                     IPWRKARLSVRPGITGLWQVCRRERDAGDFHQWIYFDVLYVRHMSLWLDLRVLLATFL
                     TMGGRFSVPLRLMIPGAEAQSVPEAFDTLGWLDGSAPRPVRGPEPAPHGEVSRN"
     assembly_gap    6846..6945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6946..7315)
                     /locus_tag="PLU99_05090"
     CDS             complement(<6946..7315)
                     /locus_tag="PLU99_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16466.1"
                     /translation="MSIQAIIMDWRPAYLQSSRSAVSTLLAPLGTETVVQHLWAQLLK
                     ADIQSSAVMPDFETDAAYQGALKAVLGEVRLCRKEGFLDFLESLEPADLLLAIDPRCY
                     PLDGYDFAGLLRGSNTCHLPR"
     gene            complement(7340..9658)
                     /locus_tag="PLU99_05095"
     CDS             complement(7340..9658)
                     /locus_tag="PLU99_05095"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOO16467.1"
                     /translation="MSDTNGKSRALARSDPVMAPQAGLETDLFELDRLGPPVRIEPDA
                     AKTFSPWLLLRYKGSILGVFLVLGALAVGGIWSMSPPRQHKATAIVEVSPVIRQLVKG
                     GSDMVPLYESFRASQVDYLTSPAVLDGAVQRMSQDERIRATNWYRDVPASPFERLLER
                     AGLRRPVPAEQRLASALSAEAPDHKQLVYVSMSTSTPGEAKLILDVVLEEYVKFALQR
                     ASDSDLEIMEQLRRQIAERATELNELEKQAANARQQLGTGTPEGLVTQGVLRLDELES
                     RMRYLETDIAWTERMLEFAESSEVNEAAAAQEALTTVNHEEDATWRSLRAELQRLRSE
                     QQVAATQFGKSHWRMVSLEQAIAVAEQALREREQELMATAAAPTANSPVQLRQSLREM
                     RIRMDVLSELLQKERQQSDQVSRSALELTELDARRQKTREMQQQLAQHLEVMEMNREV
                     AGFIRTLPATEVGATNDKRLRFMAAGLVGALAMSVGLAFVRIRFSPTVNELGDVGPAN
                     GVFLGRLPLRRGRSVMTSLEQCPIQAEAVRVVRTALLNRLSGDGGKAVQITSANVGSG
                     KSTLACLLARSLAQCGNRVLLVDADLRRPALDQRFGTDLSPGLVDVMAERTPESSAVH
                     RTSILGLSLLTAGRPQHHQEFEMLANGTLARLIHQWRKQYDLVIIDGAPLLGTADAGI
                     LSRVADGTIFVVRERHCRRAALLEGLATLSAAGGKLLGTVFVGSAGTGYGYRGRYGYG
                     YSYGYSYGYGYGFAPTAAEAVGPLDQVSRPAP"
     gene            complement(9695..10675)
                     /locus_tag="PLU99_05100"
     CDS             complement(9695..10675)
                     /locus_tag="PLU99_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOO16468.1"
                     /translation="MLAAIILAGAHVWDADSLDALCPRLLWPIGNTPLAVHQFRWLAE
                     VGIRNVVICANSDTYLYQRCFGDGQSNGVQLAYYVDRSPRGPAGCCHDAAEIVSAEQY
                     VVLEGTVLPGVDLLSLLAAQVKSKAAATVLVNRTRGAFSANGLQDGTPAGVYVFSRRA
                     FEHVRRAGFQDIKEMLIPRLRKAGEPVNAYPVESAARVSDLESYLAVQGWALQKYTHS
                     DWLPQDYAWRDGLLVHRTARVDEGVRIIGPVMVGPHSRIEESAIVVGPTVIGRGCTVR
                     AGGVVGRSVFWARCRVGVQANLDRCLVTTGASIAANGCLYGAICRAQGTG"
     gene            complement(10732..11208)
                     /locus_tag="PLU99_05105"
     CDS             complement(10732..11208)
                     /locus_tag="PLU99_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16469.1"
                     /translation="MLSRYERGNPVIRRLCLGCSEQEGERILARALASARFRPSVVVA
                     TIGVALGLIAVLADDFTWSVHAGFGWRQWGGAALASVFVFLGALVGVDFLLLAGVFLF
                     AGCVSADWLGLSRVPGFGWKQQLLLMAGAAVVGAALCGRLTGLLRWLRNSGPGSSK"
     gene            complement(12105..12584)
                     /locus_tag="PLU99_05110"
     CDS             complement(12105..12584)
                     /locus_tag="PLU99_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16470.1"
                     /translation="MHTYCDDRGRSLMAIYDHLSDLPGQINVSLMYAGAVKAWHRHRL
                     QDDHWVVTSGCLKIGLFNTEADVLTAELRLAGPGPNLDQEVRLEIPPNAGRAVYLGEH
                     RPGVLRIPARLWHGGVAVGGRDALLMYYVTRKYDPTNPDEERAAWDRFEFNWNVEYK"
     gene            12794..14797
                     /locus_tag="PLU99_05115"
     CDS             12794..14797
                     /locus_tag="PLU99_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO16471.1"
                     /translation="MWPVAPQASAGPGDALPPEFLSYRIISDSDDGTEVGRFSWSPGG
                     YLGTNLNYMGRNAAQIYDIGLRFVVPDVNQGETFVYARLALPGTEYGYVQSEVNLRIV
                     GIDADGVSSFTYQRPSRWPKTAAEVEWQHEENWPLESGSRQTPLWRYTPDLAPIINAI
                     ITRPNWGKTTGKTLGLVIENTTADGANFLAVKDYNKTLAGVVAPVLELYRTVRATFVA
                     PELLGRPTDHSITINAMSLLTLEAFVEYGLRPDRLAGRTPTALYPGGTPIEIVVDGLA
                     PNTTYYYRLRFRTPDASKFEAGPLGHFHTQRPPGSSFRFTVTSDSHLWEDVRQGTNEA
                     LYAETLRNVALDDPDFHIDLGDTLFAEDYTAGDVVDFEDSVTRLLAQRPYLHPVCRSA
                     PLFLVLGNHEGEQGWRLTAADDNVAIWATNARKLLYPNPAPDDFYTGNEDVLPWVGLR
                     EDYYAFEWGDALFVVLDPFWYTNRSPHGGGGFPSSGNNWDWTLGYTQYNWLRNVLAAS
                     EARFKFIFIHQLVGGVTIYGRGGIEAVRHVLGGFGSFEWGGEDPSGVYAFDRRRLGWG
                     DPIHRVLIDNGVTIVFRGHDHVFVRQQLDGIVYQETPSASDALYTAGLFPYQYGDVVN
                     NAGHLRVTVGPSRVRVDYIRSYLPGEGPSGEVAYTYEIPAPEP"
     gene            complement(14834..>15974)
                     /locus_tag="PLU99_05120"
     CDS             complement(14834..>15974)
                     /locus_tag="PLU99_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16472.1"
                     /translation="GLLEKTLVVIVGDHGEGLGDHGESSHSLLIYAESMNVPLLFHAP
                     GLIPAGKVVADRVVAVVDLMPTILDLLGLPITECDGVSLRLASNSDRAVYMETLAPQL
                     NHGWSPLFGLRRYWDKYIEAPQPEYYDLQTDWREMTNRWGEDSRGTMLAERLAALKSA
                     LDSGASGAATLELDTAARRKLESLGYIGSSPGTTAEGPLPDPKYMIAQSDQQMQRVSA
                     LLASGRATEAIPQIRQLLSIMPGDASVWSLLAVAHAQAGQLDEAIESRLRLLDLQPSD
                     AQNWVALATLQYAKGDVAGFEVSLAEAERLEPELGSIYMVRARRAKQEGRLEEALALC
                     EEALRRDPTRYALDSLSVQVELYRLLGRPAEAEAAGQRARQLGAP"
BASE COUNT         2959 a         5287 c         4708 g         2820 t
ORIGIN      
        1 cacttgttgc gcaacattga cgagatgtcc atgccgtgga agtgtgtcac cagcggaatg
       61 ccgaactgcc gcgcgatggg ctccacgcgc agggcgaaat ggccgaaatg cgccagaatg
      121 acctgcggcc gcgtgcggcg agccagttcg cacagaaagc ggcgctcaga cccgacactg
      181 gcgaagaagt tccagttcgg cagattacgc aggcgataca tccagcgccc tgttccttcg
      241 aacgggtgcg gctggaatgg cagcacggtc cgcgggatgt tgcacaacgg gtaggccgct
      301 tcgttcgtgt gccgccagca cacgacatgc gggcgtatgc gccgcaaccc ggtgatctgt
      361 cgccagagcc acggctcaga gggttcgccg actgcttcgg cgacgatcca tacgcgcggc
      421 gcggcggcgg tggttgtagt gagcccgtca agcatgttca gtctccacgg gcggccggcg
      481 aagtccgagc accaccagag cgcgacgcac gcgcgcccgc gtaccgaaac cgaagacggc
      541 gtcagtgaga atccgtgccg ccggcaggca taagggattg ctgcagaagg cgcgccgcaa
      601 atgcttccac cccgcgaagc gaaagccatg gtaaaaggcc tcccccgccc aggtggcgtg
      661 ccattccgcc agcgactgcg gcgccggcca gacgcgcggg cggcgactgt cacgcggcag
      721 tccgcgccgc tccagggcgt cggcgatgac cttgcgccgc aaggcattgt catcctccgc
      781 gcggccgatg gcggtaaagc tgcccgggtg cttgcgcatg gcgcctagaa catccggcaa
      841 attcgccaag cgcccgcgct cgcccagccg caagaacagg tcgtgctcct cgcaggtgcg
      901 cagtccggcc cggtagcccc cgactgccag cacgtcacgg cggcgcagca tgctcgtggt
      961 ctgtagcagc gcaagcccgc gaccttcgaa cagctcctcc tctatctctt cgtggctcaa
     1021 cttctggtcg gacatgccga ccggatcgcc atcttcatct atgaacaacg cctggccgcc
     1081 gagcacaatg cagtcggggt gggtttggag gtacgccaac tgccgcgcca cacggttcgg
     1141 catggccgtg tcatccgaat ccaaattggc aattatttcg ccccgcgccc tttcgacggc
     1201 ctcgttgcgc gtcgccggca ggccctggtt gcgggaatgg gcgatcaccc gcacgcgcgg
     1261 atcgcgctgc gcgtaggact gcgccacctc cagtgaaccg tcgctcgagc cgtcgtcgat
     1321 caccactact tcaagcggtt catacgtctg cgccagcgca ctgtcgatcg ccgcaccgac
     1381 gtagcggcgg ttgttgtaga ccggaataag aatagatacc agcaacgcca tacctcagtt
     1441 ctaagagggt gccagcgggg tttcggtcac ggcgtccagc cgcccggcat gcagccgctg
     1501 gcgcacacgt gccagggccg ccgcaagttg gcggcgttca ctggctctca ggccaagcgt
     1561 cagcacggcc aggcagtaga cggcggcgcc cgccgcaccg caggccatga ggctcggcca
     1621 actatcgacc gtcatcacca tttttggggc gtaccaggcc acgacgccga aaactccggg
     1681 cagcaggcca ggccaaaggg tcgccacgaa ccactcgcgc cagctcacac cggccatacg
     1741 caggcccagt ggccagaaca gcagaggttg ggccaccgca atcacaagca gcgtggcgag
     1801 tgccgcgccg aaagccccca tgtgcagtcg cccgaccagc agcacggccc cggccaccgt
     1861 cagaagctgc acgagcagca tccgccaagc ccaggggcgc aattcaccgg aggcgtacgc
     1921 cagacgggcc atcatgttgt tgccatactc cagtgggaag gtcagcagca atagccccag
     1981 aatggcgccg gcttcaatgt agttcgcacc ggcatagagc gttacgaact ccgtgcgata
     2041 gacgagcagt ggcaacacca gcagcatcgt tccccacagg ccgtagcgtc ccccgcacag
     2101 atacgcgcgc cgcagccgct cgcggtcgcc tgcggcgtgc aacgcggtca gcgcgggctg
     2161 cagtgggcgc gtgacgatgt actcgagcag cgcgatctgg tgaaagaaga tccagccgat
     2221 gttgaaagtg gcgacgctgg ccgcggtcga cagattctta agtatgagcg ggtgggcgcc
     2281 ggctcgaatt tggtcggcgc actgtccgat caggctccag aagccgaacc tcgtgagggc
     2341 gtgcgccgtc ggccagtgga agtcgcgcca ccgccaacgc agtgccggaa cagctcgtcg
     2401 cgaggcgtac atgagcacaa gcagcttgat gacttgcgac gtcatggttg ccgtggcgac
     2461 ccagatgacg cgtgtgttca ccccgagcag cagaacaagc agcaatgcgg cccgcaggat
     2521 ttcgccgccc agctcgatac tgttgatcca gacgaacttc tgtctcaccc ataacccgca
     2581 actgaacggc gagaccggaa acgagagggc gactggaagg atcatcatgc ccagcatcaa
     2641 gcgcgcgtcc cagacgcgct ctggttcgag ccgcagccag gcgtcgatgc gccaggcggc
     2701 ccaccagccg cccgccagca cgagcagtcc cgcggttagc aggaacggaa gcattgtaga
     2761 gacgacgcgc cgcacgccgc gctcgtcacc ctggacatgc gcctcgagca cgtagcggcc
     2821 cagcccgctg gtgagtaatg tcgccagaat gggcggaaac accattaacc ccagaatcac
     2881 tggatagaga ctgtattcct ctggggtgat ccgtttcaac aaatgctggt gcagccaaag
     2941 cagaatgacg acattcaaag ctttggccgt cacgccgctg gccgaattaa tgactaccgt
     3001 gcgtttgttg aataggtcac tcattcaggc ccggtcccgc gcggcgccgc gccggatgtg
     3061 cggcaacact ccacgcctgc aatttctttt ggctccggcg gccgaggcgc acgtgcggcc
     3121 agatagtccg ccagcatttc ccgccagctt cggcaaggcg ccaatccaag ggccgccagg
     3181 cgccggctgc ccagcgcaga gaactccggc cgacgggccg gcgcggcata atcggcggaa
     3241 ctaatcggcc gcaggtcgac cgcgcgtcct gatagtgtga atatctcgcg ggcgaactcg
     3301 aaccaagtgc aggcgccctg cgcggccgcg tgcacaatgc cgcgtgcgtc gacgcgcagc
     3361 agctcgacga ctttcgcggc gcagtcggcg gtcgccgtcg gcgacagccg ctggtcgttg
     3421 accacccgga ccttctggcc actgtccgcc aagcgcaaca tcgtttcgac gaagttgccc
     3481 ccctttccgc gggcccccgc gtggccatag agcccgcagg tacgcacgat caaggcgtct
     3541 gggcagtgcg ccagactgag gtgctcgccg gccagnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnacggc gtagcgccgg tcatgggcca ggcggtcctt tacgtaatgg
     3721 atcatgctcg catcgtgccg cagttcgcgc agcagggcgt gcgtcagctc gaggttggtc
     3781 atctcattgc cggcgccgat attgtagact tcgccgggtt gaccgcgttc cagtacagcc
     3841 agcagtgcat cacagtgatc ctgcacgtga atccagtcgc gcacgttaag tccgtcgcca
     3901 taaagcggta ccttccgctc ctccagcagg ttggttataa acagcggaat caccttctcc
     3961 ggaaactggt atggcccgta gttgttcgag caacgcgtca ccaccgccgg cagaccgtac
     4021 gtgcgatgat aggcgcgcac cagcaggtcg gccgcggctt tcgaagcgct gtacggactg
     4081 ttagggcaaa gcggcgagtc ctccgtgaag cgtccttccg gcccgagcga accgtagact
     4141 tcatcggttg acacctgcag aaagcggcgc accgcgctga gccgggactg ctcgagcagc
     4201 acttgtgtgc ccagcacgtt agtgcggatg aagtcggcgc tgccgtggat actgcgatcc
     4261 acgtgcgttt cggctgcgaa attcaccacc gccccgacct gcgtgtcgag cacggcgcgc
     4321 accgcctcgc tgtcgcagat gtcgccgcgc acgaactcga actgcgggct gtcgtccaga
     4381 ccgcgcaggt tttcgagatt tccggcgtaa gtcagggcgt cgaacgctac cacctgccac
     4441 tcgggacgtt tttcgcgaag gcggcgcacg aagttcgagc cgatgaaccc ggccgctcca
     4501 gtcaccagaa tgcggttcag catgcttgct cctcaaacgg ccgcggggac ggccgccgcc
     4561 atgggccggg tatgattcgc gccgccgccg gcggtgaggt tggccgcctt gagcagcgat
     4621 tcgaaagtac ccgcgtcggt ccaccaccct tccagaattt cgtactccag tgtgccctgc
     4681 tccaggtaaa agttgttcac atcggtaatt tccagttcac cgcgcgccga aggtttcagc
     4741 gaactaatga agtcgaacac ctgctcgtcg tacatgtaga tgcccactac ggcgtaggcg
     4801 ctttcgggca cttcgggttt ctcgacgatg cgtgtgacgc ggccgtcctc cagcacagcc
     4861 acgccgaaac gttcgggatc gggcacttcc ttcaggaaga tgcgcgcccc gcgcggctgg
     4921 ctgtagaagt tggccacacc gcggcggata tcgcgctcga tgatgttgtc gcccaacacg
     4981 actacgatct tgtctccgtc cgcaaagtct tcagccaggc gcagggcgtc tgcgatgccg
     5041 ccctcgccca cctgatatgt gtagtgcagc tctttcagtc cgaattcctt gccgttgccc
     5101 agcagacgca gaaagtcgcc ggcgtcattg ccgccggtga ccagcagtac gtcgcgaatc
     5161 ccggcgttca ccaggcattc gatggggtaa taaatcatag gtttgtcgaa gacgggtagc
     5221 aggtgcttgt tggtgatttt cgtcaggggc cacaagcgct tgcccagtcc accggccaga
     5281 attacgcctt tcatctgttt ctcttccctt ctgccatccc tctgtttgct ttagttccgt
     5341 gatacttccc catgcggcgc gggctcgggg ccgcgcaccg ggcgcggtgc gctgccgtca
     5401 agccacccga gcgtgtcgaa tgcctcagga acgctctggg cttcggctcc cggaatcatg
     5461 agccgcagcg gcacgctgaa gcgtccaccc atagtcagaa aggtggctag cagcacgcgc
     5521 aagtccaacc acaacgacat atggcgcacg taaagcacgt caaaataaat ccactggtga
     5581 aaatccccgg cgtcgcgctc gcggcggcac acctgccata gcccagtgat gccggggcgc
     5641 accgacagac gggccttgcg ccaggggatg caaatctggt tttcccgaaa aggcgacggc
     5701 cgcggaccga tcaggctcat gtggccgagc agtacgttgt acagttgcgg cagttcgtcg
     5761 atattcgtgg cgcgcaacag ctttcctaat ggagtgacgc gcgggtcatt gggcagttta
     5821 aactgcggcc catccaccgc gttcttgacg tagagggcgc gctgctgctg gtgagcgcgg
     5881 tcgatcatcg tgcggaactt ccagcagcga aatacgcggc cgttcaggcc ctcgcgctcg
     5941 tgtccaaaca gcaccggccc gcgcgagctc agcttgacca gcagcgctgt cacgcccagc
     6001 aggggcgcca gcaccaccag gccaaccagg gccgcgctaa agtctatggc gcgcttgagc
     6061 cgcggatacc agcgcttgtc gccggtcgga gcgcgagagg cagcacgccg ccgccggcgc
     6121 cgcagcaggc ggggttctga gcgctgggcc gaagcggggg cggacggttt gtggcctgcg
     6181 gcggccttgg ccggggcttg ccccaccaga acgcgccgaa caatgcagtc gccggccgac
     6241 acgcgcgccc cgggcccgaa cacgcattgg cacacgagcg agccgcgggc cacttgcgcc
     6301 cccgggccca gtaccgcggg gccgatcacg accgcatgcg gcgcgatgcg cgccccggca
     6361 tgcacgacga tcggaccata caaccggctg tgcggatcga tggagctgcc cggcccggcc
     6421 caaaggctga attcctcgct ccggcggccc gcgctgcgtc gcgcccggcg tgtactcccc
     6481 agcaccacct gctcgttcaa agccagcagc ccggcctcag tcgacagatc aaacgtggga
     6541 ccgtgcaccg tcaagtcggt gcttggtatg tcgtgagccg ccagttgacg gcgaatttgc
     6601 gcgggagaaa ggccagcgga caggtccagg tgccgcgcga ctgcggccga gatcagcgat
     6661 gcgccaatcc cgaccgcctg aacctgcgtt acgccgtcat agaggcgctg tacacagcgc
     6721 acgcgcagtt ctgaatcgta gactacgcgc tcttgtgcgc cccgcgcgtt accctgcaat
     6781 tgcaccaggt gccgcggcag atggcaagtg ttcgatcccc gcagcaagcc ggcgaaatca
     6841 tagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccgc ggcagatggc
     6961 aagtgttcga tccccgcagc aagccggcga aatcatagcc atccagcgga taacagcgcg
     7021 gatcaatcgc cagcagcagg tcggccggct ccaaggactc caggaagtcc agaaagcctt
     7081 ctttccggca cagtcggact tcgcccagga ctgctttcag ggcgccctga tacgctgcat
     7141 cggtctcgaa atccggcatc accgcagagg attgaatgtc cgctttcagt aattgtgccc
     7201 acaagtgctg gaccacggtt tccgtaccca gcggcgccag gagcgtggac acggcgctcc
     7261 ggctgctctg cagatacgcc ggacgccaat ccatgatgat cgcttgaatg gacaccgctg
     7321 cgttctccaa tcgcccgcct cagggtgctg ggcggctcac ctggtccagg ggacccactg
     7381 cctccgccgc cgttggcgca aagccgtagc cgtagccata ggaatagccg tagctatagc
     7441 cgtagccgta acgtccccgg tatccatagc cagtgccggc tgagccgacg aatacggtac
     7501 ccagcagctt tccgccggcg gcgctcaatg tggccagccc ttccagcagc gccgcccgcc
     7561 ggcaatgccg ctcgcgtact acaaaaatgg tcccatccgc gacgcgcgag agaattccgg
     7621 cgtcggctgt tcccagcaag ggagctccgt cgatgatcac caggtcatat tgtttgcgcc
     7681 actggtgtat cagccgcgcc aaggtaccgt tggccagcat ttcgaattcc tggtgatgct
     7741 gcgggcgtcc ggccgtcaag agcgacaagc cgagtatgga ggtgcggtgc accgcgctgg
     7801 actcaggcgt gcgctcggcc atgacgtcca ccagtcccgg cgagaggtcg gtgccgaagc
     7861 gctggtccaa agccgggcgc cgcaaatcgg cgtccaccag caagacgcgg ttcccgcatt
     7921 gtgccaggct ccgcgccagc aggcacgcca gcgtgctctt gccggagccg acgttggcac
     7981 tggtaatctg gaccgcctta ccgccgtcgc cactgagccg gttcagcagt gcagttctta
     8041 cgacgcgcac ggcctcagcc tgaatcggac actgctccag cgaagtcatc acactgcgcc
     8101 cgcgccgcaa cggcagacgc cccaagaaca cgccgttagc cggccccacg tcgcccagtt
     8161 cattcaccgt cggactgaac cggatacgca cgaaagccag gcccacgctc atcgccaggg
     8221 caccaaccag cccggccgcc atgaagcgca gccgtttatc gttcgtcgcg cccacttccg
     8281 tggccggcag cgtacggatg aagccggcca cctcgcgatt catctccatc acttccagat
     8341 gctgcgcgag ttgctgctgc atttcgcggg ttttttggcg ccgcgcatcc aattccgtca
     8401 gttccagagc gctgcgcgaa acctggtcgg actgctggcg ctctttctgc aaaagctcgc
     8461 tcagcacgtc catccgtatg cgcatttcgc gtaagctctg acggagctgc accgggctgt
     8521 tggccgtggg cgccgcggcc gtcgccatca gctcctgctc gcgctcgcgc aacgcctgct
     8581 cggccaccgc aatggcctgc tccaacgaaa ccattcgcca gtgcgatttt ccaaactgcg
     8641 tcgcggctac ctgttgttca ctccgcagcc gctgcagctc ggccctcaga ctcctccagg
     8701 tggcatcctc ttcgtggttg accgtggtga gcgcctcttg cgcggctgcg gcctcgttta
     8761 cttctgaact ctcggcgaat tccagcatgc gctcggtcca ggcaatatct gtttcgaggt
     8821 agcgcatgcg cgattcgagt tcgtccagcc gcaatacgcc ttgcgtgacc agcccttccg
     8881 gcgtgccggt gccgagctgc tgacgcgcat tggcggcctg cttctcaagc tcgttcagtt
     8941 cggtggcgcg ctcggcaatc tggcgacgga gttgttccat gatttccaga tcgctgtcgg
     9001 atgccctttg gagcgcgaac ttgacgtact cctccaaaac aacgtccagg atcaacttcg
     9061 cctcgcccgg cgtgctggtg ctcattgaaa cgtagacgag ctgcttgtga tccggcgctt
     9121 ccgcgctgag cgcgctcgcc aggcgctgct cggctggcac cggccgccgc agtccggctc
     9181 gctccaataa acgctcaaag ggcgaggcgg gcacatcccg gtaccagttg gtggcccgga
     9241 tgcgttcgtc ttggctcatg cgctgcaccg cgccgtcgag aacggccgga ctggtcaggt
     9301 aatccacctg gctggcgcgg aagctctcat acagcggcac catgtcgctg ccgcctttga
     9361 cgagctggcg aatcaccggc gatacttcga ctatggcggt agccttgtgc tgccgcggcg
     9421 gggacatact ccagataccg cccactgcca atgcgccgag caccaggaac acgcccagaa
     9481 tgctgccctt gtagcgcagg agcagccagg gtgaaaatgt tttcgccgcg tctggctcaa
     9541 tgcgcaccgg cggtcccagg cgatcgagtt caaacaagtc cgtctccaga ccggcctggg
     9601 gcgccattac cggatccgat cgggccaacg ctctcgattt tccgttcgtg tcactcatta
     9661 gcgcgtctcc aatgctctct ttccggtagc aaattcaacc ggtgccctgt gcgcggcata
     9721 tcgcgccgta caggcagcca ttcgctgcga tcgacgcccc ggttgtcacc aggcagcggt
     9781 ccaggttcgc ctgaacgccg acccggcagc gcgcccaaaa aaccgagcgg cccaccacac
     9841 cgccggcgcg tacggtgcag ccccgcccaa ttacggtcgg tcccactaca atggcgctct
     9901 cctcgatccg gctatgcggc ccgaccatca ccggaccgat tatgcgcacg ccctcgtcca
     9961 cacgcgccgt gcgatgcacg agcaggccgt cccgccaggc ataatcctgc ggcagccagt
    10021 cgctatgggt gtacttctga agcgcccagc cctgcaccgc caggtaagat tcgagatcgc
    10081 tgacgcgcgc agcgctttcc acgggatagg cgttgaccgg ttcgccggcc ttgcgcagcc
    10141 gtggaatcag catttccttg atatcctgga accccgcgcg tcgcacatgt tcaaacgccc
    10201 ggcgggagaa cacgtagacc ccggcgggcg ttccatcttg cagtccgttg gcggaaaagg
    10261 cgcctcgggt ccggttcacg agcaccgtcg cggccgcctt cgatttcacc tgtgccgcca
    10321 acaggctcag caaatcgact cctggaagta cggtgccttc gaggaccacg tattgctcag
    10381 cgctgacaat ctcggcggca tcatggcaac agccggccgg gccgcgtggc gaacggtcca
    10441 catagtaagc cagttgcacg ccattgcttt gcccatctcc gaaacagcgt tggtagaggt
    10501 atgtgtcgga gttggcgcag attactacat tgcgaattcc gacctctgcc agccagcgaa
    10561 attggtggac cgccaaaggg gtgttgccaa taggccatag tagacgcggg catagcgcat
    10621 ccagcgagtc tgcgtcccac acgtgcgcgc cagccaagat gatcgccgcc aacacgggcg
    10681 tttccttcgc ggttgtcggg ggcgcggaag cgctcaactg gatttggcgt atcacttgct
    10741 agacccgggc ccagaatttc gcaaccagcg caacaggcct gtcagccgcc cgcacagagc
    10801 ggcgcccacg acggcagcgc cggccatcaa caaaagctgc tgcttccaac cgaagcctgg
    10861 gacgcgtgac agaccgagcc agtccgcgct aacacagccg gcgaacaaga atacgccggc
    10921 caaaagtaaa aaatccacgc cgaccagcgc gcccaggaag acaaacacgc tggccaacgc
    10981 cgccccgccc cactgtcgcc agccgaagcc ggcatgcaca ctccaggtga aatcatctgc
    11041 caggacagca atcaaaccca gcgccactcc gatcgtcgcg acgaccaccg aaggtctgaa
    11101 acgcgccgag gccaacgctc gcgccaaaat gcgctcgccc tcttgttcac tgcatcccag
    11161 gcacaggcgc cggattaccg gatttccccg ctcgtagcga ctcagcacgt gaacactggc
    11221 aggagcacca caattgtgac aacttgggac actaaccggc agcgctgccg ccgcatccaa
    11281 atttgccgct cgttgcggcg tctgtgtatt aattgaggtt aattgccgag agggtcgtcg
    11341 ggcgacgttc ggcacttcgg atactctcat attcgcacca cccgaaataa tagaccaccg
    11401 gacgccgatg gcccgatggt ccaaggctta gccagtcttg ataccgcgcg ccacccggcc
    11461 tgtacgtcgc gcttcatccc tccctgtgcg aggaacccgt tgaacctgcg tcgggcgcac
    11521 cacaagctgc gccggcaagc gcgctcagcc ctgcccacaa cctcgtcggc gactgagaac
    11581 ctcgattcgc gctgctgttg catcgtctcc gcctgcagat tcccgattcc acatttgtaa
    11641 cgaagctccg cctcacgcgg cttcgaaaaa caacattgaa gcgcacttga accgaggagc
    11701 acactcaaag cggaacggca acttccgcca gcggcgtgcg ccgccgctgc caactggccg
    11761 ctttcaccag gccattggcg aacagtgctt actcgcatgc catgcggccc ggatgtctgt
    11821 ggacttaacg aacagcttcg gctcgaacca ttgggagtat tccgccacgt ccggcttttg
    11881 caactggccg gccacgcgac acgcgctaac gtcttacgac atcagaactt aagcgagata
    11941 gccgcccgcg tggggaccgg tgaaattcca tgttatcccc gctattcagt acattcgcgc
    12001 caccggaact aggcttgccc ccagaataga atgtatctgc tgtccccggc tcgcctacgc
    12061 agccggaaaa ccgtggcctc tgtcacacgg gaggctaccg gaccttattt gtactcgaca
    12121 ttccagttga attcgaatct atcccaagcg gcgcgctctt catccggatt agtcggatcg
    12181 tatttgcgcg tcacgtagta catgagtaag gcgtcgcgcc ctcccaccgc cactccccca
    12241 tgccaaagcc gcgccggaat ccgcagcact cccggccgat gctcccccag gtaaacggca
    12301 cgccccgcat tcggcggaat ttcgagccgc acttcctgat cgaggttcgg acccgggccg
    12361 gccaaccgaa gctcggcggt tagaacgtcg gcctcggtat taaacaagcc gattttcaag
    12421 cagccgctcg tcaccaccca atgatcgtcc tgtaaccgat gccggtgcca agccttaacg
    12481 gccccggcgt acatcaggga gacgttgatc tgccccggaa ggtcggacag gtggtcgtaa
    12541 atcgccatga gtgaccggcc acgatcgtca cagtacgtgt gcattttgga aatctccgcg
    12601 cgcccccagg ccaataattg taggcggcct ttgccttgcg cactttctgg gtgtatattt
    12661 ccttacgcag aacgtcgcgt tattctcaaa ttttcactgg gcggggcggg gcagaagcgc
    12721 ttcgcctcgg gacagtgctg gagggcaaat cgtggcctcc tttctttgga cttcttggat
    12781 ttgcttcgtc ttaatgtggc ctgtagcgcc gcaggcgtcc gcgggtccgg gcgacgctct
    12841 tccgcccgaa tttctcagtt accgcatcat ctccgattct gacgacggaa ccgaggttgg
    12901 tcgctttagc tggagtccag gaggctatct ggggacgaac ctcaactaca tgggccgcaa
    12961 cgccgcgcag atttacgata tcggccttcg gttcgtagtg cccgacgtca accagggcga
    13021 aaccttcgtc tacgcgcgcc tcgccctccc gggcaccgag tacggctatg tccaatccga
    13081 ggttaacctg cgcatagtag gtatcgacgc cgacggtgtt tccagcttca cgtaccagcg
    13141 gccctcacgc tggcccaaaa ccgcggctga ggtggaatgg cagcacgagg agaactggcc
    13201 gttggagtcg ggcagccgcc agacgccttt gtggcgttat acgcccgacc tggcccccat
    13261 catcaatgcc atcataacgc gtcctaactg gggcaagaca actggaaaaa cgctggggct
    13321 cgtgattgag aatactactg ccgacggcgc caatttcctg gcagttaagg actacaacaa
    13381 aacccttgcc ggtgtcgtcg ccccggttct ggaattgtat cggacagtcc gcgcaacctt
    13441 cgtggccccg gagctgctcg gccggcccac cgaccactcc atcactatca acgcgatgtc
    13501 gcttttgacg ctcgaagcct tcgtcgaata cggattgcgg cccgaccgat tggccgggcg
    13561 cacgccgacc gccttatacc ccgggggcac tccgatcgag attgttgtgg acgggcttgc
    13621 gcccaatact acttactatt accgcctcag gtttcgaacg cccgatgcct cgaaattcga
    13681 agccggcccc ttgggccact tccacacgca gcgtccgccg ggcagctcat tccggttcac
    13741 ggtaacgagc gattcccacc tctgggaaga tgtccgccag ggtacgaacg aagcgctgta
    13801 tgctgagacg ctcagaaacg tggccctgga cgaccccgat tttcacatcg acttgggaga
    13861 caccctgttt gcggaagact acacggccgg cgacgtagtg gatttcgagg acagcgttac
    13921 acgcctgctg gcgcagcgcc cgtatctgca cccggtgtgc cgctcggcgc cgctctttct
    13981 ggtgctgggc aaccacgaag gcgagcaggg ctggcgcctg accgcagcgg acgacaacgt
    14041 agcgatttgg gccactaacg cccgcaaact tctttatccc aaccccgcgc cggacgactt
    14101 ctataccggc aacgaggacg ttctgccgtg ggtaggcctg cgcgaagatt actacgcttt
    14161 cgaatggggt gacgcgcttt tcgtcgtcct cgacccgttc tggtacacca accgcagtcc
    14221 gcacgggggc ggcgggttcc ctagcagcgg caacaactgg gactggaccc ttggctacac
    14281 ccagtataat tggctccgca acgtactggc ggcgagcgag gcacgcttca agttcatctt
    14341 cattcaccag ttggtcggcg gggttaccat atacggccgc ggcggaatcg aggccgtcag
    14401 acacgtcctg ggcggtttcg gctcgtttga atggggtggc gaggacccga gtggcgttta
    14461 cgctttcgac cgccggcgcc tggggtgggg tgaccccatc catcgggtgc tgatcgacaa
    14521 cggcgtgacc atagtatttc gcggccacga tcacgttttc gtgaggcagc aactcgacgg
    14581 catcgtctac caggaaacac cctcggcttc agacgcctta tataccgccg gcctgtttcc
    14641 ttaccagtat ggcgacgtgg taaacaacgc cggccatctg cgtgtcactg tcgggccgag
    14701 ccgcgtccgc gtcgactata ttcggtcgta cttgcccggc gagggaccca gcggcgaagt
    14761 agcttacacc tacgagatac ccgctccaga gccgtagggg tcgcagcgct tttccctgtg
    14821 acgcctgcgc ctgctacgga gccccgagtt gacgagcacg ctgcccggcg gcctcggcct
    14881 cagccggcct ccccagcaag cggtaaagct cgacctgcac cgacaacgaa tcgagcgcat
    14941 agcgcgtcgg atcgcggcgc agcgcctctt cgcacagggc cagcgcctct tccagccggc
    15001 cctcttgctt ggcccgcctg gcgcgaacca tgtagatcga ccccaactcc ggctcgagcc
    15061 gttcggcttc ggccaggctg acttcgaagc ccgcgacgtc gcccttggcg tattgcagcg
    15121 tcgccagcgc cacccagttc tgggcatcgc tcggctgcag gtccagcagc cgcaggcgac
    15181 tttcgatcgc ctcgtccaat tgcccggcct gggcgtgcgc gacggccagc aacgaccaaa
    15241 cactggcgtc gcccggcatt attgatagga gttgccgaat ttggggaatc gcttcagtag
    15301 cccggccgct cgcgagaagg gcactcaccc gctgcatctg ttggtccgat tgcgcgatca
    15361 tgtacttcgg atccggcaat gggccttcag cggtcgtgcc gggtgaactc ccgatgtagc
    15421 ccagcgattc gagcttccgc cgcgccgcgg tatccagttc cagggttgct gctccgctgg
    15481 cgcccgagtc cagcgcgctc ttgagcgccg caagccgctc cgccagcatt gtgccgcgcg
    15541 agtcttcgcc ccagcggttg gtcatttcgc gccaatcggt ctgaagatcg tagtactccg
    15601 gctgcggggc ctcgatgtac ttgtcccagt atcgccgcag gccgaacaac gggctccagc
    15661 cgtggttcaa ctgcggagcc agcgtctcca tgtaaacggc ccggtcggag ttgcttgcca
    15721 gccgcaggct aacaccgtca cactcggtta ttggcagccc caggaggtcc aggatggtcg
    15781 gcatgaggtc aacaactgcg accacccgat ccgctaccac cttgccggcg gggattaacc
    15841 ccggggcgtg gaagagcagc ggcacgttca ttgattccgc gtaaatcagc aggctgtgac
    15901 tgctctcccc gtggtcgccc aggccctctc catggtcgcc cacgattacc acgagcgttt
    15961 tctccagcag cccc
//
LOCUS       DAOSYS010000055        15886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3072_length_15886_cov_5.397259, whole genome shotgun sequence.
ACCESSION   DAOSYS010000055 DAOSYS010000000
VERSION     DAOSYS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_15886_cov_5.397259"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..523
                     /gene="thiS"
                     /locus_tag="PLU99_05125"
     CDS             323..523
                     /gene="thiS"
                     /locus_tag="PLU99_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOO16473.1"
                     /translation="MKVTINGQARTFEQPLTIAELLAQLEVHPLRVAIEVNEQLVRRA
                     DFAATQIREGDRIEVVTFVGGG"
     gene            523..1356
                     /locus_tag="PLU99_05130"
     CDS             523..1356
                     /locus_tag="PLU99_05130"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOO16474.1"
                     /translation="MDPAPLKIAGFSFRSRLFVGTGKYLRGGEPDYELMRDALDASGC
                     EVVTVAVRRERLYDKQGRSLLDYLDTQRYTILPNTAGCFSAEDAIRAARLGRELLEGM
                     ANPGAQWVKLEVLAEKKTLLPDPIETLRAAEVLIKEGFVVMAYSNDDPVLARRLKDAG
                     VTSVMPLGAPIGSGLGILNPNNIRLILEDLKDGDPAYPVIVDAGVGTASDVTVAMELG
                     ADGVLLNTGIAGAQDPVTMATAMRHAIEAGYLAYHAGRIPRKAYAAASSPMEGRIAPA
                     K"
     gene            1403..1978
                     /locus_tag="PLU99_05135"
     CDS             1403..1978
                     /locus_tag="PLU99_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16475.1"
                     /translation="MAGTGSFDDMVAGGGPVENGGTAARVAAGQNGATVPLCPVDRLP
                     RRRRNQICIVIIAIGLLNFLIYTMTYAILGGDAHNGYRRIVTELNGAQRTEYIIRGHH
                     VRDLQGLQTPVSRGAWIYSYVHSITVPLTSGALIICMLVLARPHIITTMRDGWISGPA
                     FVTAFATVVILVSIAATVLMTAHFVAQMRGG"
     gene            2011..3984
                     /locus_tag="PLU99_05140"
     CDS             2011..3984
                     /locus_tag="PLU99_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOO16476.1"
                     /translation="MSLAEWLQPDGPIARRLPGFEIRPQQRQMSDAIAAAFERNRHLA
                     VEAGTGVGKTFAYLLPAIDQIVTNKKRVVISTHTIALQEQLIHKDIPFLREALGINFS
                     AELVKGRTNYVSLRRLKQASERQKSLFAGARLQEALHAIEDWAYETEDGSLSDLPLAP
                     PGEVWEKVRSEHGACLGRRCPLYDVCFYQRARRKAEAADLLVVNHALLISDLLLRREG
                     ASVLPEYKLAVIDEAHTLENVATDHLGINVSSTQVQYLLSGLFNERTGKGYLANTGED
                     AQKTAVIRAQSACAEFFENLHDWQRRYARSNGRLLEPPRVKNALTPALNELIETLKPL
                     KKALPREEDQYELGMYLDRAASTAESVAALLEQTHDGHVYWLEHQVGRSPRTSLCAAP
                     LDPGPFLREMVFDRVQSAVLTSATLATPPAAGRDGQGSFDYLLSRLGSPPADTLQLGS
                     PFDFERQVTIHVEAGMPDPSDSATFTEAAARAVVHYLRMTEGRAFVLFTSYQMLNQVA
                     ESVRADLQADGFTILVQGESLPRTQMLEQFRARPRAVVFGTDSFWQGVDVVGEALSNV
                     TIVKLPFAVPDRPMTEARIDLIRRRGGQPFNDYQIPEAILKFRQGFGRLIRSRTDRGI
                     VVILDPRVVSKPYGRRFLESLPKCRLEVSRQPW"
     gene            4097..4750
                     /locus_tag="PLU99_05145"
     CDS             4097..4750
                     /locus_tag="PLU99_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16477.1"
                     /translation="MTRIESGQQLCRTCLRRLRTPKERNPDAPPSDELICEAYRLGVN
                     FPAGATHGQVLRMVRLARRWLPVTEEFLNADFDRLVRFQDDVISYVADAWEQITGVRP
                     AQPEIPGPEQQRFAAWLILKRRPMALAMQRVEMQRQRFRRELEAEHAATAPGTPFDWD
                     AVKPPIAEDETYACLVPILRSEWSEYLPHRPSRFARWLRRRKEGGGDDGERTEHEGD"
     gene            4785..5162
                     /locus_tag="PLU99_05150"
     CDS             4785..5162
                     /locus_tag="PLU99_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOO16478.1"
                     /translation="MSPEQRMVREFHEKYSAPVRSTPTLISPKDRLRRARLIFSEAAE
                     FIEAADKEDLPEMVDALADLLVVVYGAALELGVDLEPVFAEVHRSNMSKNGGKDAGGK
                     ILKGPDFTPPDIIGELRKQGYRP"
     gene            complement(5312..6634)
                     /locus_tag="PLU99_05155"
     CDS             complement(5312..6634)
                     /locus_tag="PLU99_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004067418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOO16479.1"
                     /translation="MNSAKRKLIILGAAGRDFHDFNTYWRDNPDVEVVAFTATQIPDI
                     HGRTYPAVLAGPKYPKGIPIYAEEELPELIRRHGVDLCTIAYSDLPHEVVMHKAALVN
                     AAGADFIILGHKHTMLKSRLPVIAVCAVRTGAGKSQTSRAVVRVLKKLGKKVAAVRHP
                     MPYGDLTKQICQRYATYEDLDRYECTIEEREEYEPHIDAGNIIYAGVDYARILAEAEK
                     EAEVILWDGGNNDMSFYKPDLYITVLDPHRPGHERRYYPGETNLYMADVIVLNKMNTA
                     RPEDIDTVLKSIQEVNPRASVVRANSPVTVDDPAAIRGKRVLVVEDGPTLTHGEMKYG
                     AAHVAARQNGAAAVVDPRPHAVGSIKGTFEKYRHLTDILPAMGYGRKQIQELEQTINA
                     VDCDVVLIGTPIDLRRVLKINKPTVRVSYELEEIEPGQLEAAIRKVIR"
     gene            7443..7820
                     /locus_tag="PLU99_05160"
     CDS             7443..7820
                     /locus_tag="PLU99_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008425739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOO16480.1"
                     /translation="MSGKNRVKAKTIDEYLAALNADQRAALERLRKVIRAAAPQAEEC
                     ISYQLPAYRCNGMLVAFGAAAKHCAFYPMSSSTVEDLKDELKAYETSKGTIRFQPEKP
                     LPAALVRKIVKLRLAENAARGRA"
     gene            8045..8656
                     /locus_tag="PLU99_05165"
     CDS             8045..8656
                     /locus_tag="PLU99_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16481.1"
                     /translation="MNANQTGAAADRPSAPPTTAGPLPPPETSATWATVIGVVAIVLG
                     VLGTFGGIWGILSPLLMQYFDQVMPGNVHVTETTRAWAAWTISLSAVAAVLGLLLLAG
                     GVGLVRRRHWSRAALICWALLKIILAVANGLVVWAVQQHALQEQSRMMGPGFAPGGPG
                     ATTVEAVAVLGMALTILWGWALPVFLLIWFSRRSIKAQMAQWR"
     gene            8691..10133
                     /locus_tag="PLU99_05170"
     CDS             8691..10133
                     /locus_tag="PLU99_05170"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO16482.1"
                     /translation="MRCKQCDYRLWNLTARRCPECGTPFLPSEFEFVPNSVQFCCPHC
                     GQAYYGTDAKGHLVPPAFTCGRCGAAIQMDEMILLPATGLHEEQTRVSRMPWLDWRNR
                     GLVRAWLATVGAALTTPGRLMRLLPADAPMWPARGFALLTLFVTATVAVGPFMIFPLV
                     VSPRSGAVQILLGTVIALLIAFGLLSLTTLVWGLVTHGLLRLTGRTAGNSGRTMQAIY
                     YSTGANILTAIPCLGVYLGWIWWMVSAVLMVREAQRVHGGRAALAVVLPPLLALSGLV
                     GGYVYLFVAVLRAPSTAASPIFVARQVQTATVLHAVLDYAARHTGQGPLHASELVASG
                     RLPAGHFALSKSATSTAAVPVSGTTLDALEALPYEEAQAVVAAAAAALPEGTVAHRVG
                     DFVFVYHGADISAAAGGIWVVVAAPDALADPASQALFAGHADGSITRIDPGGLAAALT
                     EQNALRAESGLPPLSDPTTVTHERPAVCLP"
     gene            10394..11983
                     /locus_tag="PLU99_05175"
     CDS             10394..11983
                     /locus_tag="PLU99_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16483.1"
                     /translation="MRPGQRSRHDRSGKGAVGLFHWHIQENRIASTPMANRLLGLPAD
                     QPISFADLRERIHPDDRELTIAALDEALSAAGECDLEFRIVLPDKSERWIGFIGTVAR
                     GPQGFPEHMAGVVLDVSERRRHESERDALLAAERAARHEAERQVRLKDEFLARLSHEL
                     RAPLTTMLGWAQMLRRGKLGSAEMGEAIKSIESSVYIQKRLIEDLLDISRITSGKLRL
                     NVEPLDLGEVVEAAAASISPMAEAAGVTLERKVKGDLGPVLGDRTRLQQVIWNLLSNA
                     VKFTKSGGWVRLTARREPDRVVVVVADNGIGIAGEQLGRLFERFHQAAPADRGGLGLG
                     LAIVKELCDMHGGSVRAESPGVGRGATFTLELPIAALGPGGFSGLPHAPGELNAAACD
                     VSVLAGLRVLWVEDEPDTRDVVARILREYGTKVRTAGSVAAALAELEADPPDVLISDI
                     GMPHEDGCTLVRKIRQHKSERIAKIPAIALTALARPEDRERTLAAGYQIHVAKPVDPA
                     MLAQAVCRVVTAQPPGGHGRR"
     gene            12324..13508
                     /locus_tag="PLU99_05180"
     CDS             12324..13508
                     /locus_tag="PLU99_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOO16484.1"
                     /translation="MTRTIVTMPGDGIGKVVLPEALRVLDAAGFKADYVNADIGWDFW
                     ITEGNALPERTIAALEKHKIGLFGAITSKPKDKADLELTPALRGKGLTYFSPIVTMRQ
                     HFKLDICIRPCRSFAGNPLNFIRRKPDGGFEEPPVNAVVFRQNTEGLYAGVEWTNPPD
                     NLRAAFATNKKWRPFAETPGADLSVSLRVITREATRRICRAAFEYARKFGYRSVTICE
                     KPNVLRETSGMFTQEAKRVAQDFSGIALWETNIDAQMMWLTKNPEDYGVIVCSNMFGD
                     IASDAFAGLVGGLGFACSANIGEEVAVFEPTHGSAPKYEKLDPPIVNPIAMILSACMM
                     LDHIGETAKSDKIKGAIAEVVAEGRVRTYDMLRLPGGPDVFKKGAASTTQMTDAIIAK
                     LK"
     gene            13638..14024
                     /locus_tag="PLU99_05185"
     CDS             13638..14024
                     /locus_tag="PLU99_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOO16485.1"
                     /translation="MQDFQRLDVWQRAHKLTLAVYRTTSEFSDVERFGLARQVRRATS
                     SIGANIAEGCGRDTHADFARFLQIAAGSASELANHLLLARDLGYIKRDALRPLWSELA
                     EVRRMLTALIKRVRTTNTRNITNTNN"
     gene            14096..>15886
                     /gene="lysF"
                     /locus_tag="PLU99_05190"
     CDS             14096..>15886
                     /gene="lysF"
                     /locus_tag="PLU99_05190"
                     /EC_number="4.2.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoaconitase"
                     /protein_id="HOO16486.1"
                     /translation="MPQTLVEKIAQKYAVGLPAGHEVHAGDFLSISPLHCMTHDNTDA
                     VIPKFKSIGAKKVHNPRQPVFTLDHDIQNKSAENLAKYAKIEAFAREHGIDFYPAGRG
                     IGHQIMLEEGYALPGAFVVASDSHSNIYGGLGALGTPVVRTDAAAIWATGRTWWQVPE
                     IVKVTLTGRLTNGASGKDVIISLIGTFKNDDVLNCCLEFAGPGVATLTMEERFAISNM
                     TTEWGALAAVFPYDDVTRQYLLERAEVMRRRGDREPRLTPEKVRQLESELPTADPGAF
                     YAKEIEFDLGTVSPYVAGPNEVKAIAAARELEAKNVRIDKAFLMSCVNGRLQDFAAAA
                     EVLRGQKIAPHVKLYVAAASSEVEAQAKERGYWSTLADAGATFLPAGCGACIGLGEGV
                     LTDGEVGISATNRNFDGRMGSRKSQVYLASPAVVAASAVAGKIAAPKAAAATTPKPTG
                     AVKANPRPAAGAAKVEILPGFPQQVAGELLFVPKDNMNTDGIYGKEYTYKTLPPEEMG
                     KVAMANYDPEFQKIARQGDILVGGYNFGSGSSREQAATSLKFRGLQLVVAGSFSQTYS
                     RNAYNNGYICIECPALVNDLRAAFTAGCKDG"
BASE COUNT         2889 a         4820 c         5275 g         2902 t
ORIGIN      
        1 tggggccgct gagcaatgag gattttctga taacggccgt gcgggcgcgc tgaaaatccg
       61 cctcgggcct atcgtgtcga gccgcttccg gcagccgaaa tccgaggcgg cgcgtcgtgc
      121 gcgctctaat tccggagtgt tcggattgcc tttctccgcc tcccacgcac ggcgaccctt
      181 ctggctggcc cttcgcgctc gatcgctggc tggagcttcg tgctcgggat gaatgtataa
      241 tccgcgccgg tgttatatga aggtcaccat taacggccaa gcgcggaacg aggagcgaac
      301 atgaaacagc gaggaaagtt atatgaaggt caccattaac ggccaagcgc ggaccttcga
      361 gcagccgctc acgattgccg agttgctggc tcaactcgaa gtccacccgc tgcgcgtggc
      421 aattgaagtg aacgagcagc tcgtccgccg ggctgatttc gcggctactc aaattcgaga
      481 gggcgaccgc atcgaggtcg tcacgtttgt tggaggaggt tgatggatcc cgctccgctc
      541 aagattgccg gcttttcctt ccgctcgcgc ctctttgtcg gcaccggcaa ataccttcgc
      601 ggcggcgagc ccgactatga gttgatgcgc gacgctttgg acgcctcggg gtgcgaggtc
      661 gtcacggtcg ccgtgcgccg cgagcggctg tacgacaaac aaggccgcag cctgctggac
      721 tacctggaca cgcagcgcta cacgatcttg ccgaacacgg ccggctgctt cagcgcggag
      781 gacgcgatcc gggcggctcg cctggggcgc gagctgcttg agggcatggc caatccgggg
      841 gcgcagtggg tcaagctcga ggtgctggcc gagaagaaga cgttgctgcc agacccgatc
      901 gagacgttgc gggcggcgga agttctcatc aaagagggct tcgtcgtaat ggcttattcc
      961 aacgacgacc cggtgctcgc gcggcggctg aaggacgcgg gcgtaacgag cgtaatgccg
     1021 ctgggggcgc cgatcggcag cggcctggga attctgaacc cgaacaacat ccgccttatt
     1081 ctcgaggatt tgaaggacgg cgatccagcc tacccggtaa tcgtggatgc cggcgtggga
     1141 acggcgtccg acgtgaccgt tgcgatggaa ctcggcgccg acggcgtcct actgaacacc
     1201 ggcatcgccg gcgcccaaga tccggtgacg atggcgactg cgatgcgcca cgcgattgaa
     1261 gccggctatt tggcctatca tgccggccga atcccgcgca aggcgtacgc ggcggcttcg
     1321 tcgccaatgg aagggcgcat cgccccggcc aaatagacgt cggccaaata aacgtcggcc
     1381 aaatagacga aagaggtacg ctatggcagg caccggctcg ttcgacgaca tggtggccgg
     1441 cggcggtccg gtcgaaaacg gcgggacggc cgcacgtgtc gccgcgggcc agaatggggc
     1501 caccgtaccg ctgtgcccgg tcgaccggct gccgcgccgc cgccgcaacc agatctgcat
     1561 cgtcatcatc gccatcggcc tgctgaactt tctgatctac accatgacgt acgcgattct
     1621 gggcggcgat gcgcacaacg gctatcgccg cattgtcacg gagctgaacg gagcccagcg
     1681 cacggagtac ataatccgcg gccaccacgt gcgcgacctg caggggttgc aaacgccggt
     1741 cagccggggg gcgtggatat atagctacgt gcactcaatc accgtgccgc tgacgtccgg
     1801 cgcgttgatc atctgcatgc tggttctagc gcggccgcac atcattacca ccatgcgcga
     1861 tggctggatc agcgggccgg cgttcgtgac ggctttcgcg accgttgtaa ttctggtatc
     1921 gatcgcggct accgttttga tgacggcgca ttttgtcgca caaatgcggg gcggttgaga
     1981 ccagccatca attggcagga gtagcccggc gtgtctctgg cggagtggct gcaaccagac
     2041 ggaccaatcg cccgccggct gcccggcttt gaaatccggc cgcagcaacg ccagatgtcg
     2101 gacgctatcg ccgccgcttt cgagcgcaac cggcatctgg cggtggaagc cggcacgggc
     2161 gtgggcaaga cgttcgccta tctgctgccg gcgatcgacc agattgtgac gaacaagaag
     2221 cgggtcgtga tcagcacgca cacgatcgcg cttcaggaac aactgatcca caaagacatc
     2281 ccatttctgc gtgaagcgct ggggatcaac ttctcggccg aactcgtcaa aggccgcacg
     2341 aattacgtga gcctgcggcg gctgaagcag gccagcgaga ggcagaagag cctgttcgca
     2401 ggcgcgcggc tgcaggaagc cctgcacgcg atcgaggatt gggcctatga aaccgaggac
     2461 ggctcactca gcgacctgcc gctggcgccg ccgggcgagg tatgggagaa ggtgcgcagc
     2521 gagcacggtg cctgcctggg gcggcgctgc ccgctttacg acgtctgctt ctaccagcgt
     2581 gcgcggcgca aagccgaggc ggcggatttg ctggtcgtta accatgcgct gctgatcagc
     2641 gacctgttgc tgcgccgcga aggagccagc gtgctgccgg aatataagct ggccgtcatc
     2701 gacgaagcgc acacgctgga aaacgtggcc accgaccacc tgggcatcaa cgtctccagc
     2761 acgcaggtgc aatatttgct cagcgggtta ttcaacgagc gcaccggaaa gggctacctt
     2821 gccaataccg gagaagatgc ccagaagacg gccgttatcc gggctcaatc cgcctgtgcc
     2881 gagttcttcg agaacctgca tgattggcag cggcgttacg cccgttcgaa cggccggctg
     2941 ctggaaccgc cgcgagtcaa gaacgcgctg acgccggcgc tgaacgagct gatcgagacg
     3001 ctcaagccgc tcaagaaagc gttgccgcgc gaagaggatc agtacgagtt gggtatgtac
     3061 cttgaccggg cggcgtcgac ggccgaatcc gtggccgcgc tgctcgaaca gacccacgac
     3121 gggcatgtgt actggctcga gcaccaggtg gggcgttcgc cgcggacgtc gctatgcgcg
     3181 gcgccgctcg acccggggcc gttcttgcgg gaaatggtct tcgatcgcgt gcaaagcgcg
     3241 gttctgacca gtgctaccct ggcgacgccg cccgcggcgg gccgcgacgg ccaggggagc
     3301 ttcgattacc tgctaagccg cctgggttcg ccgccggccg atacgcttca actcggttca
     3361 ccgttcgact tcgagcggca ggtgacgatt cacgtcgagg ccggcatgcc ggatccgtcc
     3421 gacagcgcaa ctttcacgga ggcggcggcc cgcgccgtgg ttcactatct gcgcatgacg
     3481 gagggacgtg cgttcgtgct gttcaccagt tatcagatgc tgaaccaggt ggccgagagc
     3541 gtgcgggcgg atctgcaggc cgatggcttt accatcctcg tgcaaggcga gtcgctgccg
     3601 cgcacacaaa tgctggaaca attccgggcg cggccgcggg ccgtggtttt cggtaccgat
     3661 agtttctggc agggggtcga cgtagtgggc gaggcgctct cgaacgtcac gattgtgaag
     3721 ttgcccttcg cggttcccga ccggcccatg acggaggcgc gcatcgacct gatccgccgc
     3781 cgtggcgggc agcccttcaa cgattatcag atacccgaag ccattctgaa atttcgccaa
     3841 ggcttcggcc ggctcatccg cagcaggacg gaccggggca tcgtagtgat tctcgacccg
     3901 cgcgtcgtat ccaagcccta tggccggcgt ttcctggaga gcctgccgaa atgccggctg
     3961 gaagtatcgc gccagccgtg gtgatactcc caccttgacc tcgctccaag gctccccaag
     4021 gatatgggta gggatggtaa gaacatttcg tcgagaccgt ggtcgctgcg attcgtgcgg
     4081 caagcagccg cgggtagtga cgcgtattga aagcgggcag cagctttgcc gtacctgcct
     4141 gcgccggctg cgcacgccga aggagcggaa tcctgacgcg ccgcccagtg atgaactgat
     4201 ttgcgaggct tatcggctgg gggtgaattt tcccgccggc gcgacgcacg ggcaggtttt
     4261 gcggatggtg cgtctggcgc ggcgctggct gccggttact gaagagttcc tcaacgcgga
     4321 tttcgatcgc ctggtgcggt tccaggacga tgtgatctcg tacgtcgcgg acgcgtggga
     4381 gcagattacc ggcgtgcgtc cggcgcaacc cgagataccc gggccggagc aacagaggtt
     4441 cgccgcgtgg ttgattctca agcgtcgacc gatggcgttg gccatgcagc gggtggagat
     4501 gcagcgccag cgttttcggc gggagttgga ggctgagcac gccgccacgg cccccgggac
     4561 tccgttcgac tgggatgcgg tcaaaccgcc gatcgccgaa gacgaaactt acgcctgcct
     4621 ggtacccatc ttgcgcagcg agtggagcga atatctgccg catcgtccga gtcgtttcgc
     4681 ccgctggctg cgccgccgga aggagggagg cggggatgac ggtgagcgaa ctgagcacga
     4741 gggggattga gcgaggcgcg ggggggggcg agtgaggaag gctaatgagt ccggaacaac
     4801 ggatggtgcg ggagtttcac gagaagtaca gcgccccggt gcgaagcacg cccacgctga
     4861 tcagccccaa ggaccgcctg cggcgcgccc ggctgatctt cagtgaggcg gccgagttca
     4921 ttgaggcggc agataaggaa gatttgccgg aaatggtgga cgcgctcgcg gatctactgg
     4981 tggtagtgta cggcgcggcg cttgagctgg gagttgacct cgagccggtc tttgccgagg
     5041 tgcatcgttc caacatgtcc aagaacggcg gtaaagacgc cggcggcaag atcctcaaag
     5101 ggcccgattt cactccgccc gacataatcg gcgaactgcg caagcagggt taccggccgt
     5161 aggctggttg attcggggct gaagtacccc cagtactgac gtccataaaa gcggcgggag
     5221 gacgaagccc ggctgatccg caccggcttc gccctcccac gcaacgctat ctggagtttt
     5281 tcggcagccc gcgcgatgga cgcgctgctg actaccgaat caccttccga atcgcagctt
     5341 caagctgccc gggctcgatt tcctccagct cgtagctgac acgcaccgtc ggcttgttga
     5401 ttttcagcac gcgccgcaag tcgatcggcg taccgatcag gacaacgtcg caatcgaccg
     5461 cattgatcgt ctgctccagc tcctggatct gcttgcgccc gtaacccatg gccggcagaa
     5521 tgtccgtcag gtgccggtat ttctcgaacg tgcccttgat tgagccgacc gcgtgcgggc
     5581 gcgggtcgac gacggccgcc gcgccgttct gtcgcgccgc gacgtgcgcc gcaccgtact
     5641 tcatctcgcc gtgcgtgagg gtcgggccgt cctccacgac caggacgcgc ttgccgcgaa
     5701 tggccgcggg atcatcgacc gtcacgggcg agttggcccg cacgaccgag gcgcgcgggt
     5761 tcacttcctg gatgctcttc agcacggtgt cgatatcttc gggccgtgcg gtgttcatct
     5821 tgttcagcac gatcacgtcg gccatgtaga ggttcgtctc gcccgggtaa tagcggcgct
     5881 cgtgaccggg ccggtgcgga tccagcacgg tgatgtacag gtcgggcttg tagaacgaca
     5941 tgtcgttgtt gccgccgtcc cagaggatga cctcggcctc tttctcggcc tcggccagga
     6001 tgcgggcata atcaacgccg gcgtaaatga tgttgcccgc gtcgatgtgc ggctcgtatt
     6061 cctcgcgttc ctcgatggtg cactcgtacc gatcgaggtc ctcgtacgtg gcgtaacgct
     6121 ggcaaatctg cttggtcagg tcgccgtagg gcatgggatg ccggaccgcg gccactttct
     6181 tgcccagctt cttcagcacg cggaccacgg cgcgtgaggt ctggcttttt cccgcgccgg
     6241 tgcggaccgc gcaaaccgcg atcacgggca gacgcgactt cagcatggtg tgcttgtggc
     6301 ccaggatgat gaagtcggcg ccggcggcgt taaccagggc ggccttgtgc ataaccactt
     6361 cgtgcggaag gtccgagtag gcgatggtgc acaggtcgac gccgtggcgg cggatcagct
     6421 ccggcagctc ctcttcggcg tagatcggga tgcccttggg gtacttggga ccggccagga
     6481 cggctggata ggtccggccg tggatgtcgg gaatctgcgt agcggtgaag gcgacgacct
     6541 cgacgtctgg gttgtcgcgc cagtaggtgt tgaagtcgtg gaaatcgcgc ccagcggcgc
     6601 ccaggatgat aagtttccgt ttggcggaat tcatggagcg tactcacgta tccggtcggg
     6661 gagcgtccag caagaacccc tacgggggac agcggccgac gcctgccaga ggccgccgtc
     6721 gttgaccata cgggcgcgat ttcgacacac ccgcatccga ataacgtacc gcgattgggc
     6781 gtaaggctca agagtgggca tgcgcagcgg tgtagttgag cagagccggg gcgtgccgcc
     6841 ccggaatgat gccaacgcgt cccaattcgc ctttgtgcgg gcagagccgg gggcgtgccg
     6901 ccccggaata ccgcccagaa cctcgcatcc gcgcagccgt ccatccaact ctggcatccc
     6961 acgatgcgag tccgacccga gtcggcggga cctgatccga gcccgaagcg ccagcgaggg
     7021 cagccgaccc gtagcgtcgg ccccccgtgg ccgacgtcgc acgcctggcg cgcagctccc
     7081 cgtttcacac caccatcccc cccttcgcac cgccgaagcg ccgcaagcag acggcgcccc
     7141 gggagcaaac agggcaggct ctggcacggc ggcaggggct ctgcgcgctg taagcagtcc
     7201 aattagccac ggcgttcctg cctgattctg acaatccagg tggtggaact taagcccggg
     7261 acgtacacgc tggggatttg ggcgaacgtt ggcaatagcc aagcgtcccg ctggcttgac
     7321 gtatggaatc gcatcgcact caatgtcatc gggtcgccgc ggcctatcag gcctgaaagc
     7381 cttctttgat aaacggtctg tttgaaaaca aagacagaga tcacgttacg gagttcgcga
     7441 ctgtgtccgg taagaacaga gtcaaagcga aaacgatcga cgaatacctc gccgctttga
     7501 atgctgacca gcgtgccgca ttggagcggc tgcgcaaggt cattcgcgcc gcggctccgc
     7561 aggcagaaga gtgcatcagc taccagcttc cggcataccg ctgcaacggc atgctggtgg
     7621 ccttcggcgc ggccgccaaa cactgcgcct tttatcccat gagttcttcc acggtcgagg
     7681 acctcaaaga cgagctcaag gcctacgaga cgagcaaggg tacgatccgc tttcaacccg
     7741 aaaagcccct gcccgcggca ctggtgcgca agatcgtgaa gttgcgcctc gcggagaacg
     7801 ccgctcgcgg cagggcttag ctgaggtcgg gttcccctcc gcagaacatc gggcgcgctc
     7861 gcaggcgccg ccataagcct cgctcgcccg gcggctattc ggggcgatct cagcgcgccg
     7921 ggattcccag gctgcttgtg ccggaccagt tgccagatcg ccgcggcctt cgcatcatga
     7981 cgtgtgccag cgcgagttca atgacggtcc gcaactagca cccgcctctt gcggaggttt
     8041 tatcatgaac gcgaatcaaa ccggagctgc ggcagaccgg ccatcggcac ccccgacgac
     8101 cgccggcccc ctgccgccgc cagaaacgtc cgcgacctgg gcgaccgtga tcggggtcgt
     8161 ggcgattgta cttggagtcc taggcacgtt cggcggcatt tggggcatac tgagtccact
     8221 gctgatgcag tattttgacc aggtcatgcc cgggaacgtg catgtcacgg aaacaacgcg
     8281 tgcatgggct gcatggacta tttcactttc ggcagtggcg gccgtactgg gcctactgct
     8341 gctggcgggc ggcgtcgggc tggtgcggcg ccggcattgg agccgcgcgg cgctgatatg
     8401 ctgggccttg cttaagatca tactcgcagt ggcaaacggg ttggtggtct gggccgtgca
     8461 gcagcatgcg ctgcaggagc aatcgcgtat gatggggccc ggcttcgcgc cgggcggtcc
     8521 gggcgccacc acagtggagg ccgtcgcggt tctcggtatg gccctcacga tcctgtgggg
     8581 ctgggcgcta ccggtgtttc tgcttatttg gttctcgcgc cggtccatca aagcgcagat
     8641 ggcgcaatgg cgctgactga ccactaatga ctgataactg agaaactgca atgcgctgca
     8701 agcaatgtga ttaccgctta tggaatctca ccgcccggcg ctgcccggag tgcgggacgc
     8761 cctttctgcc cagcgagttc gaattcgtcc ccaacagcgt gcagttctgc tgcccgcact
     8821 gcggccaggc gtactacggc acggacgcga aggggcacct cgtcccgccg gcgttcacct
     8881 gcgggcgctg cggcgcggct attcaaatgg acgagatgat tctcctgccc gccaccggct
     8941 tgcacgaaga gcagaccaga gtatcgcgca tgccgtggtt ggattggaga aaccgcgggt
     9001 tggtcagggc ttggctggcg accgttgggg cagcgctgac aacgcccggg cggctgatgc
     9061 gcttactgcc cgcggacgcc ccgatgtggc cggcgcgtgg cttcgcgctg ctcacgctct
     9121 ttgtgaccgc gaccgtcgct gtggggccgt tcatgatttt cccactggtt gtgtcgccgc
     9181 gctccggtgc agtgcaaatc ttgttgggaa cggtcatcgc gcttctgatc gcattcggat
     9241 tgctaagtct gacgacactg gtgtgggggc tggttacaca cggtctgctg cgcctcaccg
     9301 gcagaacggc cggcaattcc ggccggacca tgcaggcgat ctactacagc acgggggcaa
     9361 acattttgac ggccataccg tgcctggggg tctacctggg gtggatctgg tggatggtaa
     9421 gcgcggtgct gatggtgcgt gaggctcagc gggtacatgg cgggcgggcg gccttggcgg
     9481 tcgtgctgcc tccgctgctc gcgttaagcg gtctggtagg ggggtacgtc tacctgttcg
     9541 tggctgtgtt acgggcgcca tcaaccgccg ccagcccaat cttcgtcgcg cgccaagttc
     9601 aaactgcgac ggtgctgcat gcagtgttgg actacgcagc tcggcacacc gggcaggggc
     9661 cgctgcacgc atcagaacta gtggcctcgg gtcgtcttcc ggccggccat tttgctctat
     9721 cgaaaagcgc caccagcacg gcggctgtgc cggtcagtgg aacaacgctg gatgccctgg
     9781 aagcgcttcc gtacgaggag gcgcaggcgg tcgtggccgc ggctgccgcg gcactgcccg
     9841 agggaaccgt agcgcatcgg gttggagatt tcgtctttgt ctatcatggc gcagacattt
     9901 cggcggctgc cggcggaata tgggttgtag tcgcagcccc cgacgcgttg gccgacccgg
     9961 ccagccaggc cctgttcgcc ggccatgcgg atggaagcat aacccgtatc gacccgggcg
    10021 gcctggcggc ggcattgacg gagcagaacg cgctgcgggc cgaatcgggg ttgccgcctt
    10081 tgtctgaccc gacgaccgtt actcacgaac ggccagcggt ttgcttgccc tagtgcgcag
    10141 ttctccgact tgcagcgttt gagaaattca atgttgcgcg cggactggag ccgcgttgga
    10201 tttgcgattg cgcaaagtgg caattcgcat tttgcagaat tcacttgact aagtgcttgt
    10261 ggggtgtgat cgtagttgcg agcgcactgg gctcggcgac gagaattttc tattcgagac
    10321 gaagcaaggt tggggaactg tggttcaacc cttgccagac gtgggcggaa gcggacgcgg
    10381 cgcagaatgg gccatgcgtc cggggcagcg ctcacgccac gaccgttcag gcaagggcgc
    10441 cgtcgggttg ttccattggc acatccagga gaaccgcatc gcctccaccc ccatggccaa
    10501 tcggctgctg ggactccccg ccgatcagcc catcagcttc gccgacctgc gcgagcgtat
    10561 tcaccccgat gaccgcgagt tgacgatcgc ggctctcgat gaagcgctgt ccgccgccgg
    10621 cgaatgcgac ctcgagtttc ggatagtctt gccggacaaa agcgagcgct ggatcgggtt
    10681 cattggcact gtggctcgcg gaccgcaagg cttcccggag cacatggcgg gggtggtgct
    10741 ggatgtgagc gagcgccggc gtcacgaatc cgagcgagac gcgctgctcg cggcggagcg
    10801 cgccgctcgt catgaggccg aacgtcaggt gcggctcaag gacgagtttc tcgcccgcct
    10861 atcacacgag ttgcgcgcgc cgctgaccac gatgctcggc tgggcccaga tgctgcgacg
    10921 cggcaaactg gggtcggccg agatgggcga agccatcaaa agcatcgaga gcagcgtcta
    10981 tatccagaag cggctgattg aggatctgct tgacatcagc cgaattacgt cgggcaagtt
    11041 gcgcttgaat gtcgagccac tggatttggg cgaggtggtg gaagccgcag ccgcttcgat
    11101 cagccccatg gctgaagcgg ccggggtgac gctggagcga aaggtcaagg gcgacctcgg
    11161 tccggtactt ggcgaccgga cgcgcttgca gcaggtaatt tggaacctgc tctctaacgc
    11221 cgtgaaattc accaaatcgg gcgggtgggt gcgactgact gcgcggcgtg agcccgatcg
    11281 ggttgtagtg gtcgtggcgg ataacggcat cggcattgcc ggcgagcaac tgggacgcct
    11341 gtttgagcgc tttcaccagg cggcgccggc cgatcgtggt gggttggggc tgggcctggc
    11401 gatcgtgaag gagctgtgcg atatgcacgg cggttcggtg cgtgcggaga gcccgggcgt
    11461 tggccggggc gcgacgttca cgttggaact gcccatcgcg gcgctgggac cgggcggctt
    11521 ttccgggttg ccgcacgcac ccggcgagct gaacgcggcg gcttgcgacg taagtgtgct
    11581 agccggtctg cgcgtgctat gggtcgagga cgagcccgat acacgggatg tcgtcgcccg
    11641 tattctgcgc gagtacggca cgaaggtgcg aaccgccggc tcggttgcgg ccgcactggc
    11701 cgaactggaa gccgacccgc cggacgtatt gataagcgac atcggcatgc ctcacgagga
    11761 cgggtgcacg ctggtgcgga agattcggca gcacaagtcg gagcgtattg cgaaaatccc
    11821 cgctatcgcg ttgacggcgt tagcgcgtcc ggaggatcgg gagcgaaccc tcgcggcggg
    11881 ttaccagatt cacgtcgcca agcctgttga tccggcgatg ctggcgcagg cggtctgccg
    11941 tgtggtcacg gcgcagccac ccggggggca cggccgccgg tagccctgct gcgggtgtcc
    12001 ccgaaatcca aacacgtgct aactgcatct gcctttccca ccgcggggtt tttataagac
    12061 gtattcggcc gcctgctctc tcctcccgtc ccgctgaggc cgcatcgcga tcacgccact
    12121 tgcgtcacca agtggcatga cgcctgcttc cagtggacca ctggcctctg ccaacgtgcg
    12181 actgcgccgg cttcggcttt cctgacaccc catgccggca acggcgcggt atagttacgg
    12241 gttcgcaccg agcgccgctg ccgcccgagt ccgctggcag ggctcggcga gcgcggcgag
    12301 tcttgttcct ggaggtgact tacgtgacgc ggacaatcgt gacgatgccc ggcgacggga
    12361 tcggtaaagt ggtactgccc gaggcattgc gagtgctgga cgccgcggga ttcaaggcag
    12421 attacgtcaa cgccgacatc ggttgggatt tctggatcac ggagggcaac gcgctgccgg
    12481 agcgcaccat tgcggcgctg gaaaagcaca aaatcgggct tttcggcgcg atcacctcga
    12541 agcccaagga caaggccgac ctggagctga cgccggcgct gcgtggcaag gggctcacgt
    12601 atttcagccc gatcgtcacc atgcggcagc acttcaagct tgacatctgc atccggccct
    12661 gccggtcgtt cgcgggcaat ccgctgaatt tcattcggcg caagccggac ggcgggttcg
    12721 aggagccgcc ggtgaacgcg gtggttttcc ggcagaacac ggaggggttg tacgcgggcg
    12781 tggaatggac gaatccgccc gacaacttgc gtgcggcatt tgccacgaac aagaaatggc
    12841 gcccgtttgc ggagacgccc ggagcggatc tgtccgtttc gctgcgcgtc attacccgtg
    12901 aggctacgcg gcgcatctgc cgggccgcgt ttgagtacgc ccggaagttc ggttaccgat
    12961 cggtcacgat atgtgagaag ccgaacgtgc tccgcgaaac cagcggcatg ttcacgcagg
    13021 aagccaagcg cgtcgcgcag gactttagcg ggatcgcgtt gtgggaaacc aacatcgacg
    13081 cccagatgat gtggttgacc aagaacccgg aagattacgg ggtgatcgtg tgcagcaaca
    13141 tgttcggcga catcgcgtcg gacgcgttcg ccggcctggt cggcgggctc ggtttcgcct
    13201 gctcggccaa catcggcgag gaggtcgcgg tatttgagcc gacgcacggc agcgcgccga
    13261 aatatgagaa gctcgatccg ccgatcgtga acccgatcgc gatgatcctc tcggcctgca
    13321 tgatgctgga tcacatcggc gagacggcca agtcggacaa gataaagggc gcgattgccg
    13381 aagtggtggc cgagggccgc gtccgcacgt acgacatgct tcgcctgccg ggcgggccgg
    13441 acgtattcaa gaaaggcgcc gccagcacga cgcagatgac ggatgcgatt attgcgaagc
    13501 ttaaatgagt aattagtaat tagtagttag tattggcgcg ctgtcgcctg ctggtagtta
    13561 ggtgttgacg cgtttgtcgc ctgttggttg tttgcggttg cggttgattg ctgcggctgc
    13621 tgagaggtgc ttttccaatg caggacttcc agcgattgga tgtctggcag agggctcaca
    13681 agctcacctt ggcggtttat cgaacgacgt ctgagttttc cgacgtcgaa cggttcggct
    13741 tagcgcggca ggtgcgacgc gcaacttcct ctattggcgc caacatcgcg gaaggctgcg
    13801 ggcgcgacac gcatgccgac tttgcgcgat tccttcaaat cgcagcaggt tcggcgagcg
    13861 aactggcgaa tcatctgctg ctggcccgcg atttgggcta tattaagcga gatgcgctgc
    13921 gccccctctg gtcagagctc gcagaagtga gacgcatgct cacagcactc ataaagcgag
    13981 tgcgaaccac caacacgcgc aatatcacca atactaacaa ctaataacta aaaactttat
    14041 tgccaactat cgaccaaaac cttagccagc aagcgtgaac caaggaacca ccaccatgcc
    14101 ccaaacactc gtcgagaaga tcgcccagaa atacgccgtt ggattgcccg ccggccacga
    14161 agtgcacgct ggcgatttcc tctcaatctc gccgctgcac tgcatgacgc acgataacac
    14221 tgatgcggtc ataccgaagt tcaagtcgat cggcgccaag aaagtgcata atccgcggca
    14281 gccggtgttt acactggatc acgatatcca gaacaagtcg gccgagaacc tggccaagta
    14341 cgccaagatc gaagccttcg cacgcgaaca tgggatagac ttctaccccg ccgggcgtgg
    14401 tattgggcat cagattatgc tcgaagaggg ctacgcgctg cccggcgcgt tcgtcgtcgc
    14461 ctccgattcg cactcaaaca tctatggcgg cctcggcgcc ctcgggacgc cggtcgtgcg
    14521 cactgacgcc gcggccatct gggccaccgg ccgcacctgg tggcaggtgc ctgaaattgt
    14581 gaaggtgact ttgaccgggc gtctgaccaa cggcgccagc ggcaaggacg tcatcatctc
    14641 gctcatcggc acgttcaaga acgatgacgt gcttaattgc tgcctcgaat tcgccggacc
    14701 gggcgtggcg acgctgacga tggaggagcg gtttgcgatc tccaacatga cgaccgaatg
    14761 gggagcgctg gcggccgttt tcccctatga cgacgtcacg cggcagtact tgttggagcg
    14821 ggctgaggtc atgcggcggc gcggcgaccg cgagccgcgc ctcacgccgg aaaaagtgcg
    14881 gcagctcgaa agcgaactgc ccacggcgga tcccggggcg ttctacgcca aagaaatcga
    14941 attcgacctc ggaacggttt cgccctacgt ggccggcccg aacgaggtga aggccatcgc
    15001 cgcggcgcgc gagctggaag caaagaacgt ccgcatcgat aaggccttcc tgatgagctg
    15061 cgtgaacggg cggctccagg acttcgcggc cgcggccgag gtcctgcgcg gccagaaaat
    15121 cgcgccgcac gtgaagctct acgttgcggc cgcttcgagt gaggtcgagg cgcaggccaa
    15181 agagcgcggc tattggagca cgctggcgga cgccggcgcc actttcctgc cggctggttg
    15241 cggggcgtgc atcggcctcg gcgagggcgt gctgaccgac ggcgaggtcg gtatctccgc
    15301 cacgaatcgc aacttcgacg gccgcatggg ttcgcgcaaa tcgcaggtct acctggccag
    15361 tcctgccgtc gtggcggctt cggccgtcgc gggcaagatt gccgcgccaa aggcggccgc
    15421 cgccacgacg cccaagccaa ccggcgcggt taaggcgaac ccgcgcccgg cggccggtgc
    15481 ggccaaggtc gaaattctgc ccggcttccc gcagcaggtt gcgggcgaat tgctgtttgt
    15541 gcccaaggac aacatgaaca ccgacggcat ttacggcaag gaatacacct acaagacgct
    15601 gccgccggaa gaaatgggca aagtcgcgat ggccaactac gaccccgagt tccagaagat
    15661 cgctcggcag ggcgacatcc tggtcggcgg ctacaacttc ggcagcggct cttcgcgcga
    15721 gcaggccgcg acctcgctga agttccgcgg cttgcagctt gttgtggccg ggtccttctc
    15781 gcagacgtac agccgtaacg cgtataacaa cggctacatc tgcatagagt gcccggcgct
    15841 ggtcaatgat ttgcgggctg cgttcactgc gggctgcaag gacggc
//
LOCUS       DAOSYS010000056        15858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3081_length_15858_cov_5.145922, whole genome shotgun sequence.
ACCESSION   DAOSYS010000056 DAOSYS010000000
VERSION     DAOSYS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15858
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3081_length_15858_cov_5.145922"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            425..2632
                     /locus_tag="PLU99_05195"
     CDS             425..2632
                     /locus_tag="PLU99_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOO16487.1"
                     /translation="MKPLLRTVVLLASLAFAQFALADPIRYDGNRVVSVQVGSQDDLA
                     RLLTMTDDVWSESVGLGRVDVRVTPEQFQRLRESGLPFEVMIFDLQELIDREAAENAA
                     GRTAFDHYMNLTELTAYLETLVSLRPDLAQLFVAGQSAQGRDITGIRITGPGTGLKPG
                     ALIHGGQHAREWITVPVTVYLADQLIRQYDSNPEIQALVDRFEWHLIPTMNPDGYVYT
                     WTAERMWRKNRRAMTGGTVGVDLNRNWAVGWGGAGASSFPADETYRGPAPFSEPETQV
                     IRDYLLEHPDIVAYCDLHSYGQLIMYPFGYVAASPRYPERTTYQYLAPLMASTLWAVH
                     HTSYSYGPVCTTIYPAGGVSVDWVYDVAHRYAFTYELRDTGQYGFLLPASRITATCEE
                     TFPSLLAYVSNLYPDCNENGVYDRQDIVAGTSSDCNANDVPDECEPGGASDCNGNGVP
                     DLCDLYFATSYDCNENGVPDECDIAGGTSSDCQPNGIPDECDLAPPTSAVAADSCAAA
                     QLVCAGAYYGTTVGAAQDGSSSCDGPSDAPDMWYYYQPQGNGFLTVTLAGSSFDTLLS
                     IHSGCPGTSDNEIVCIDGPELVVFVMSGHSYWIRISGAGGATGEFQMSLVGPACAWQA
                     ECNDNGVPDECEPDCNGNGQPDDCDIAQGISHDTDGDGLPDECESAAPGDTNCDGVVN
                     TFDIDPFVLALTSPAEYLAVHPGCPIGNADANGDGSINAFDIDAFVQLLLGAR"
     gene            complement(2667..3638)
                     /locus_tag="PLU99_05200"
     CDS             complement(2667..3638)
                     /locus_tag="PLU99_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO16488.1"
                     /translation="MPVEQTTTPDGETIAASGFRQADGPTTGLICSPDGYILTSSFNF
                     VRDPPVITATLADGRRFVARLVARDRAARLALLKIEAAGLPVCQWCPADQMRVGQWTL
                     LAGYGHGGDAPALSVGVLSALSRLNGRALQTDARASPANYGGPLFDLEGRVLGICVPR
                     AGRGEDEVAGVEWYDSGVGFAIRNDYIQQVLPLLRAGRDFEQGSFGLRVQAADPVIGG
                     HPDEAPLGGVRLVEVLAGGPAAEAGLLAGDLITAIGRQPTVRPIDVLRAVARIPAGER
                     VTITYIREGVERTCTLLTGDVKEAARQQQSPAASQPEQAEEQPPAAP"
     gene            complement(3866..4891)
                     /locus_tag="PLU99_05205"
     CDS             complement(3866..4891)
                     /locus_tag="PLU99_05205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOO16489.1"
                     /translation="MIKPGRSWLAFVLVGLLAVRAADAGESLPQEPLAPAIAAASERV
                     VKLYGAALGREKAYGSGVLVSADGQIVTALSLILEGRALRAVLPDGRAFPAQVERRDE
                     QRQLALLKIDAVNLPYFDVGDNAEPAVGDWLIAASNAFKVADGPEPVSISIGVLSGRT
                     NLAARLRAQDFPYDGPVLLADMIISTPGSAGGAVVDREGRLVGLIGRSVISKRTNTWL
                     NYALPSQEVAAFLRGQKYQPRAAQAGTGAQSAQTPLAMGIRLFDIGGRTRPAYVERVR
                     PNSPAAWAGILPDDLVLSVNGETVGTCDELRQAFARAPAGVPLTLVVKRGETLYVCEL
                     VVDPEGI"
     gene            complement(4888..6465)
                     /locus_tag="PLU99_05210"
     CDS             complement(4888..6465)
                     /locus_tag="PLU99_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO16490.1"
                     /translation="MTARDVALLLTIAGVSLAVPGPAPARADIQPGDAAALATVRADE
                     AQRIAAIAQVMPAVVCVVEDRQRTGGGSGVLIDSEGHGLTNFHVIAPMLESRSGYGGM
                     SDGNLYPLRVVGIDPGGDIALFKLEGKERFDFAPLGDAEQLHVGQWVAALGNPFALAE
                     DYNATVTVGVVSALHRYQYGQGNLLEYADCVQVSTSINPGNSGGPIIDLNGRVVAIAG
                     RGSFEERGRVNVGIGYGVPVNQVRRFLPALRAGRLCYHGTLGATAELLGDDLILNVVP
                     EGSPAAAAGLEFGDKLLRINGRPVRTENELTNAIAVLPAGWPVALDLLREAKPLTVRL
                     RLEALALAGMPVFVPDLQLNHAEIRRVFGLYERHDANISQSPLPEPPTFRVRCQSGVG
                     PMQDGWPSPAIAEEYEQLAAGLLGERALDVTWELLGGDEVGDRLVTVIEHRLPTGRHV
                     RWKLSCDSHELCAATIGTAEQPEAVTWEPGPRRDFGALRWPAHWVRRSPGSEDMVVEI
                     EAVHGALPAATATEAAS"
     gene            complement(6497..7741)
                     /locus_tag="PLU99_05215"
     CDS             complement(6497..7741)
                     /locus_tag="PLU99_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOO16491.1"
                     /translation="MFQQASRSAVLVALLFAAGVAAIAHARQPALEVMRLDGQTEVGS
                     LVRLVPEVSLEVAGGVISLPWSEILAIRPSDASPSSQPSTANPQPLQATLADGSVLAG
                     EVTAGDQYGFTFRFGEGEECRLELSQVCVIRALAASAAAEQKLSELLRLRKDQPGAEP
                     AEDHVTDVAVVARGSEVLVLNGRILEVQPQQVAFEWKGTQRPIPWNRLGGLIFARPEL
                     RRAPCIVRLNTGDAIAGQVVGGASGVLKLRMAVLERELDIPWASVERVDCRSERLVVL
                     SDLQPAGYEFEPFFDKNWNYAVDRTLTGRQIRLGGRAFARGLSMHSRARLVYQLDGQF
                     ERFAATAGILDEMGGRGCVTLRVRGDGRVLWSATGVRGGEPPRDVSVDIAGVRELSLE
                     VDYDADLDLSDQAAWGFARLIR"
     gene            complement(7899..9263)
                     /locus_tag="PLU99_05220"
     CDS             complement(7899..9263)
                     /locus_tag="PLU99_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO16492.1"
                     /translation="MSLSAAEQLTIPVCQPVSFVQPKPWLRWAAVVIAAAGWYVSYRA
                     FGVSAQTGGLDPLLTKFCGGPDESGLDNCGAVLTSPQAYLALGPNAGSLRIPTATFGM
                     AYFAALLVWYLFIGPPTYERRKWFLVPALVLIGGIIYSVSAIQVMAQVLNRWCPICIA
                     AHTLNGGLVLLSLIAWPWRRPRREVLPHPTARLALAVILAGVLLLLVHLLVVFVQVVG
                     SITRERMAEYTRIVGDPEFVRWDFQRQAAADIPLYADEVFAGNPDAAHTVVVFGDFQC
                     TVCKQSHEMLMEVARKYAGQIRIAFRYYPQDTACNPNPRFRTGGHFSACRAVAAAEAA
                     RAVGGLEAYLNMRALLWERQNLLPKVPLPQQSPAQLELFESWAAELNLDGAAFQQALE
                     SPVIADRVRSDIELANKLGISAVPVVFLDGKRILGWSKLETWEALLGELPVPTSQPES
                     APSR"
     gene            complement(9281..11011)
                     /locus_tag="PLU99_05225"
     CDS             complement(9281..11011)
                     /locus_tag="PLU99_05225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOO16493.1"
                     /translation="MRHRHAGSFEAASTKPTLAGGRARPAGWSLAISFVLAGLIVSAV
                     MAVRSTGVHHEDDLTHLQIARWAWQHPAYLLDDWGRPGFTALYALPAGLGWLPARLFS
                     GLLTAASAWLAYLVAVQLRVRLAAFVPLFLWIQPLSFTLSYTTLTETALAFYLSLACW
                     LYVRGRFAWSAAAISLCAITRHEAALFLVMWLAALLYRARPAREWLWVFWAPLLHNLL
                     AFAFLREWPLKLLLQPHPTSEYGQGSLLTMIVLWVLAAGFGTLLLAIIGAPVLARRRG
                     GLLFVSSAAAYVAAHSVMYGFGLFGSGGYARFLVPVGPVVAVSAAAALSEYRRAWRRW
                     AAGPQNLPLLNANAIGAAAVIVFWGAAGLELHRLRADLIINHLEWTVPVLHAAALMLM
                     LLFFTVLVLSSQQKMARPTLALTVLPITALAAALLQPLVVSSIPRPFRQCAPLQLTDR
                     EKAHRTAAEIARAATGSPSDHQTTGAAIFSASPWIDEFVGRVRPPVEPDLAQRWESSR
                     AGDVLIWDARHAASPRHRLPLSALTADPDLVELWRSTGDAYEPVYCAVLQKRPQREAP
                     RQRPDATGPN"
     gene            11396..11782
                     /locus_tag="PLU99_05230"
     CDS             11396..11782
                     /locus_tag="PLU99_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOO16494.1"
                     /translation="MPFEDKTIVCADCGQEFVHSADDQARYAERGFTNEPRRCRACRD
                     KRKTEGGRGGGGGGGGERGGGRSSSGGPRGGGRGMQQSRESFEVVCSECGTTTTVPFK
                     PSGDRPVYCRDCFQKRRGGSRSGRGR"
     gene            complement(12084..12254)
                     /locus_tag="PLU99_05235"
     CDS             complement(12084..12254)
                     /locus_tag="PLU99_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16495.1"
                     /translation="MLYVLGSSMMLMGVPIAALVSIAAVRTPGQFRWGVTALVISAAE
                     ALALLLMFAGVL"
     gene            complement(12755..13774)
                     /locus_tag="PLU99_05240"
     CDS             complement(12755..13774)
                     /locus_tag="PLU99_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO16496.1"
                     /translation="MAVRDAGQVEQQVAEFRTQFAALKAEIQKMIVGHNDVVEAVLVC
                     LFAGGHALLEGVPGLGKTLLIRTLAEALSLDFARIQFTPDLMPADIIGTNIITEDPQT
                     GRRQFQFTRGPLFAQIVLADEINRATPKTQSALLEAMQEHTVTSGGTKYTLKEPFFVL
                     ATQNPIEQEGTYPLPEAQLDRFFFKLIVGYSDRQELKTILDRTTTGYKPEVQHVLTGE
                     QIIAGQDLVKRVVIAPHVQDYVIRMVLATHPNGAFATPSVNKYVRWGSSPRGAQALTL
                     AAKIYALLDGRYNVGFEDVKKAAPHALRHRLLLNFEGEAEGLSTDIIVQDIMSSLPQM
                     AEKAA"
     gene            complement(13919..15133)
                     /locus_tag="PLU99_05245"
     CDS             complement(13919..15133)
                     /locus_tag="PLU99_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO16497.1"
                     /translation="MNEPGNGQREIEYITTSDGARLAVKRRPGRGAPVIFLHGLGVNA
                     DLWDLPSITGPDYEYHSLASILSAAGYDLWLVNFRGHGAPHMLSLPPPALDDWSVDHF
                     IVFDMPAVVEHVLAATGRRPFVVGASMGAMVLAGFAQGAQLIGGGAEAHIVADAELAA
                     RRAEQLAGAVFVEFPAALCWPSSACDASGRVNWKALLRDFLRTDADANYPFELLARWA
                     WLQALIAEAGHVPLGWMRGEGRQSWWTKLPPALADTLAEAQCKLVQGLLGVAGRFTGA
                     TNYRAEVLLQGRRYIVDHMKAGVLAQMAKSVRAKAFVSTLGSPDHVYSDNYANVSVPT
                     LVVAGGRDRIANAAVTREVFFERIQARDKTFRLYPEIAHGEFEAAPIATAQVYPEIKS
                     WLDDRDRRTHKA"
     gene            complement(15117..>15858)
                     /locus_tag="PLU99_05250"
     CDS             complement(15117..>15858)
                     /locus_tag="PLU99_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOO16498.1"
                     /translation="TKEASDMVLTDDNFATIVNAVEQGRTIYDNIRKFVHYLLSANAG
                     EVLLMFFGALLGWPAPLVPIQILWINLVTDAFPALALGLEPPEPDIMSRPPRPPREAV
                     ISLRRGGLILFHGGLIAAAGALAFHLTYAGDPQNLAVARTVTFGTLAYAQLAFALGCR
                     SDRYTLPRLGLLSNPWLLAAIAFSGLLQLAALTIPALRPIFQVSGVGGPAWLLIAGLA
                     IAPLIVVEAAKLAANLVPPERNRHERAR"
BASE COUNT         2931 a         5144 c         5196 g         2587 t
ORIGIN      
        1 tagtgggcga gtagccgccg ccatgacctg atgggttcgg ttcgcgttgc cacgaatgtg
       61 gcaacgcggc cgagccggcg gcttgccccg gttcgctgtg gcgaactgcg tgcatgccgg
      121 aaaagaaaga cagtgatcga gcgaaggctg gacgtgtaag cggtccggcc ttcgctcgtc
      181 gcgttactgg gagctgcagg tgatggcggc gtcgccacgc ttcaaaggcc aagactcagc
      241 aatgctgggg gtgagcgccc ggccatgact ccacgtctgc gtagacaaaa aacgagttta
      301 cgaattccta cagtacacac accgcacacc gagcgccgct cgtatttgcg cttcaaattc
      361 cccgcctggc gctggtaaaa tggtcgttac gaggttgtga ctcacggggg accaggagcg
      421 ggcaatgaag cctcttcttc ggaccgtcgt gctactggcg tctttggcgt ttgctcagtt
      481 cgcgctcgcg gacccgatcc gctatgacgg aaatcgggtc gtcagtgtac aagtcgggag
      541 ccaggatgat ctggcgcgcc tgctgaccat gaccgacgac gtgtggagcg agagcgttgg
      601 gctggggcgc gtggatgtcc gcgtcactcc cgagcagttt cagcgcctca gggaatccgg
      661 actgcctttt gaagtcatga tcttcgatct gcaggagctg atcgaccgcg aagccgccga
      721 aaacgcggcc ggccggactg cgttcgacca ctatatgaac ctgacggagt tgacggccta
      781 tctggagacg ctggtgagcc tgcggccgga cctggcccag ctttttgtgg ctgggcaatc
      841 ggcgcaaggc cgcgatatca ccggcattcg cattaccggg ccgggtacgg ggctcaagcc
      901 gggcgctctg attcacggtg ggcagcacgc gcgcgagtgg atcacggttc ccgtgacggt
      961 gtacctggcc gatcagttga ttcggcaata cgacagcaat cctgagattc aagcgctggt
     1021 tgaccgcttc gagtggcacc tgataccgac gatgaatccc gacggctacg tttacacctg
     1081 gaccgcggag cgcatgtggc gaaagaaccg ccgcgcgatg acgggcggta cggtgggggt
     1141 cgacttgaac cggaattggg ctgtaggctg gggcggtgcg ggtgcgagca gctttccggc
     1201 cgatgaaacc tatcgcgggc cggcgccctt ctcggaacct gaaacccagg tcatcaggga
     1261 ttacctcctg gaacacccgg acatagttgc gtactgcgac ctgcatagct acgggcagct
     1321 catcatgtac ccgttcggct atgttgcggc ttcgccgcgc tacccggagc ggaccacgta
     1381 ccagtacctt gcgccgctga tggccagcac tctatgggcg gtacaccaca cgtcctattc
     1441 ctatggtccg gtatgcacca cgatatatcc ggctggcggc gtatcggtcg attgggtcta
     1501 cgatgtagcg caccgttacg cgttcaccta cgaactgcgc gacaccggac agtatggttt
     1561 cttgctgccg gcgtcacgga ttacggcgac ctgcgaggag acattcccgt cgctgctggc
     1621 ctatgtttca aacctctatc ccgactgcaa tgagaacggc gtctatgatc gccaggacat
     1681 tgtggctggg acgtcgagcg actgcaacgc caacgatgtg ccggatgaat gtgagccggg
     1741 cggagccagc gactgcaacg ggaatggagt tcccgatctg tgcgaccttt attttgccac
     1801 tagctacgac tgcaacgaaa acggcgtacc ggatgaatgt gacatagccg gcggcacgag
     1861 cagcgattgc cagccgaacg gcattccgga cgagtgcgat ctcgcgccgc cgacgagcgc
     1921 cgtggctgcg gacagttgtg cggccgcgca actcgtctgt gccggcgcct actatggtac
     1981 aactgtcggc gcggcgcagg acgggtcatc cagttgtgac ggcccttctg atgcgccgga
     2041 tatgtggtac tactatcagc cacaaggcaa cggcttcctg acagtcacgc ttgccggttc
     2101 gagctttgac acgctgctat cgatccacag cggctgcccg ggcacttcag acaatgagat
     2161 cgtctgcatt gacggaccgg aactggtggt tttcgtcatg tccggccatt cttattggat
     2221 cagaatttcc ggcgccggcg gcgccacggg cgaatttcag atgtcgctcg tcggcccggc
     2281 gtgcgcgtgg caggccgaat gcaacgataa cggggttccc gacgagtgcg agccggactg
     2341 caatggcaac ggccagcccg acgactgcga cattgcgcag ggcatcagcc acgacaccga
     2401 tggcgacgga ctcccggacg agtgcgagag tgctgcgccg ggtgatacga actgcgacgg
     2461 tgtcgtgaac acttttgaca tagatccgtt cgtgctggcg ctcaccagtc ctgcggaata
     2521 cttggcagtg catcccggtt gcccgatcgg aaacgccgat gctaatgggg acggaagcat
     2581 aaacgcgttc gacattgacg cgttcgtgca gcttctgctc ggtgcgcgat aacagcgggc
     2641 cggcggcgct ggtcggggcg atcggtttag ggagcggccg gcggctgctc ctcagcctgt
     2701 tccggctgag aagcggctgg tgactgctgc tgacgcgccg cttctttcac gtcgcccgtc
     2761 agcagtgtgc acgttctctc aacaccctct cgaatgtacg tgatagtgac gcgctcgccg
     2821 gctggtatgc gggccaccgc gcgcaagacg tcgataggac gcaccgtggg ttgccggcca
     2881 atggcagtga tcaaatcgcc cgccagaagc ccggcttcgg cggccgggcc gccggccaac
     2941 acttccacca ggcgcactcc gccgagcggc gcctcatcgg ggtggccgcc gatcaccgga
     3001 tcggccgctt gcacgcgcag gccaaacgag ccctgttcga agtcgcgtcc ggctcgcagc
     3061 agcggcagga cttgctggat atagtcgttt cgaatcgcga agccgacgcc cgaatcgtac
     3121 cactccacac cggccacttc gtcctcgccc cggccggctc tcggaacaca aatgccgagc
     3181 acgcggccct cgagatcgaa tagcgggccg ccgtaattgg ctggcgaagc gcgcgcgtcg
     3241 gtctgcaaag ctcggccgtt cagccggctg agcgcactca ggacgccgac cgacagcgcg
     3301 ggtgcgtccc cgccgtgccc atagccggcc agtagcgtcc attgcccgac gcgcatttgg
     3361 tcggccgggc accactggca gacaggcaat ccggcggcct caattttgag caaggccaag
     3421 cgcgcggcgc gatcgcgggc caccaggcgc gccacgaacc tgcggccatc agccagggtc
     3481 gcggtgatca cgggcgggtc gcgaacgaag ttgaaactgc tggtgaggat gtatccgtcc
     3541 ggcgagcaga tcaggccggt ggtcgggccg tcggcctggc gaaaaccgga tgccgcgatc
     3601 gtctcgccgt ccggcgtggt ggtctgttcg acgggcatgg cgccaccgat ggtttcgatt
     3661 cgaaccagcg ccggcgcgac gttctgtaaa gctgcacgga aagccgcgga ctgtgctgcg
     3721 cgcaacgacg cgcctgattc gctaaccggc ggcgaatcgt cagctctggc gccggcgtga
     3781 aggcctagcg cggcggctgt cgccagaatg agccatgtgc gggcagttgg gaggtggata
     3841 gcggcatcgc gccggctgta cggcctcata ttccctccgg gtcaacaacc aactcgcaca
     3901 cgtagagcgt ctcgccgcgc ttgacgacca gggtaagggg cacgccggcc ggcgcgcggg
     3961 caaatgcttg ccgcagctca tcacacgtgc ccacggtttc gccattcacc gacagcacga
     4021 ggtcatcggg aaggataccg gcccaggccg cgggcgagtt cggccgaacg cgctcgacat
     4081 atgccgggcg ggtgcgcccg ccgatgtcga acaggcgtat gcccatcgcc agtggcgttt
     4141 gcgccgactg cgcacctgtg ccggcctgcg cggcgcgggg ctgatatttc tggccgcgca
     4201 ggaacgcggc cacttcctgg gacggcaaag cgtaattgag ccaggtgttg gtgcgtttgc
     4261 tgatcacgct acggccgatc agacccacca ggcggccttc gcggtcgacg accgcgccgc
     4321 ctgcgctacc gggagtggag atgatcatgt ccgccagcag caccggcccg tcataaggaa
     4381 agtcctgcgc tcgcaaacgt gcggcgaggt tggtgcgacc cgacagtacg ccgatcgaaa
     4441 tagacaccgg ctccgggcca tccgccacct tgaaggcgtt ggacgcggcg atcagccagt
     4501 cacccacggc cggctcggcg ttgtcaccca catcaaaata gggcagattg accgcgtcga
     4561 tcttcaacaa cgcgagctgc cgctgctcat cgcggcgttc cacctgggca gggaaagctc
     4621 gcccatccgg caggaccgcc cgcagcgccc gcccctccag gatgagcgaa agggcggtga
     4681 ctatctggcc gtcggctgag acgagcacgc cggacccgta agccttttcc cgcccgagtg
     4741 ccgcgccgta cagcttgacc acgcgctcgc tagccgcagc gattgccggg gccaacggct
     4801 cttgcggcag gctttcaccc gcgtccgcgg ctcgcacggc cagaaggccg acgagaacaa
     4861 atgcgagcca cgatctgccc ggcttaatca cgacgccgcc tccgtcgcag tcgctgcggg
     4921 tagcgcgccg tgcaccgcct cgatttcgac gaccatgtct tcggaacccg gtgagcgccg
     4981 cacccagtgc gcaggccaac gcagggcgcc aaagtcccgt cgcgggcccg gctcccacgt
     5041 aacggcctcg ggctgctccg cggtgccgat ggtcgcggcg cacagctcgt gcgagtcgca
     5101 actcagcttc cagcgcacgt gccgtcccgt gggcagccgg tgctcgatga ccgtgacgag
     5161 gcggtcgccc acctcatcgc cgcccaacaa ttcccatgta acatcgagag cacgctcccc
     5221 gagcaagccg gccgccaatt gctcgtattc ttcggcaatc gcggggctgg gccagccgtc
     5281 ctgcatcggt ccgactccgc tctggcagcg cacgcgaaag gtcggcggtt ctggaagggg
     5341 gctttgagag atattcgcat cgtgtcgctc atacagccca aatacgcggc gtatctcggc
     5401 atgattgagc tgcaggtcgg gaacgaacac cggcatgccg gcgagcgcca gggcttccag
     5461 ccgcagtcgc acggtgagcg gtttagcctc gcgcagcagg tcgagggcaa cgggccagcc
     5521 ggcaggcaga acagcaatcg cgttggtgag ctcgttctcg gtacgcacgg gccgcccgtt
     5581 tatcctcaac agcttgtcgc cgaactccaa ccccgcggcc gccgccggcg aaccctccgg
     5641 cacgacgttc aagatcaggt cgtcgcccaa aagttcggcc gtggccccga gcgtcccgtg
     5701 atagcagagg cgtccggccc gcagcgccgg caaaaagcgc cggacctggt tgaccggtac
     5761 gccgtagcca atgcccacgt tcacgcggcc gcgctcttcg aacgagccgc gcccggcgat
     5821 ggcgaccacg cgcccgttga ggtcgatgat cggtccgccc gagttgcccg ggttgatcga
     5881 agtggaaacc tggacgcaat cagcgtattc gagcagattt ccctggccgt actggtagcg
     5941 gtgcagtgca ctaacgacgc ctactgtgac ggtcgcgttg tagtcctcgg caagggcgaa
     6001 cggattgccg agagcggcga cccattgacc gacgtgcaat tgctcggcgt cacccagcgg
     6061 ggcgaagtca aaccgctcct tgccttcgag cttgaaaagg gcgatgtcgc cgccgggatc
     6121 gatgccgacc acgcgcagcg ggtagaggtt gccatcggac atgccgccgt agccggagcg
     6181 cgactcgagc atcggggcga tgacgtgaaa attggtcagg ccgtggcctt cggaatcgat
     6241 cagcacgccg ctgccgccgc cggtgcgctg gcgatcctcg acgacgcaga ccacggcggg
     6301 catgacctgt gcgatggcgg caatgcgctg cgcctcgtcg gcgcgaacgg tggcgagagc
     6361 ggcggcatcg ccgggttgga tatctgctct ggcaggcgcc ggccccggaa cagccaagct
     6421 gacgccggcg atcgttaata gcagcgcgac atctctcgcg gtcaaccgag caactccagt
     6481 atcgcccgcg ttcgcctcac cgaatcaggc gtgcgaagcc ccaggccgcc tggtccgaca
     6541 aatccaggtc cgcatcgtag tccacctcca gcgacagctc gcgtaccccg gcgatgtcga
     6601 ccgatacatc acgcggtggc tcgccgccac gtacgcccgt ggcgctccac agcacgcgcc
     6661 cgtcgccacg cacgcgcagc gtcacgcagc cgcggccgcc catttcgtcc agaatgccgg
     6721 ccgtcgcggc gaagcgctca aactggccgt cgagctgata caccaggcgc gcgcggctgt
     6781 gcatgctcaa accccgcgcg aaagcccgcc cgcccaggcg gatctgccgc ccggtgagcg
     6841 tgcggtccac ggcgtagttc cagttcttgt cgaagaacgg ctcgaactcg taacccgccg
     6901 gctgcaggtc gctgagcacg accaggcgtt cgctgcggca atccacgcgc tcgaccgaag
     6961 cccacgggat atccagctcg cgttccaaaa cggccatgcg gagtttcagt acgccgctgg
     7021 cgccgccgac cacctgcccc gcgatggcat cgcccgtatt gaggcgcacg atacacggcg
     7081 cgcggcgcag ctccgggcgg gcgaagatca ggccgcccag gcgattccag ggaatgggtc
     7141 gttgcgtgcc cttccattca aaggcgactt gttgcggttg gacctcaaga atgcgtccgt
     7201 tcagtacaag gacctccgag ccgcgcgcca ctaccgccac gtcggtcaca tgatcttcgg
     7261 ccggctcggc cccgggctga tccttccgga gccgcaggag ttcggacagc ttctgctctg
     7321 ccgcggcgga tgcggccaac gcgcggataa cgcagacctg cgacaactcc agccggcatt
     7381 cctccccttc cccgaaccgg aaggtgaagc cgtactgatc tccggccgtt acttcgcccg
     7441 cgaggactga gccgtcggcc agcgtggctt gcaggggctg cggattagcc gtgctcggtt
     7501 gagaagaagg gctagcgtcg gagggcctga tggcgaggat ttccgaccag gggagcgaga
     7561 tcacgccgcc ggcaacttcg aggctgacct cggggaccag gcggacgagt gatccgacct
     7621 cggtttgccc gtccagccgc atgacctcga gggcgggctg ccgggcgtgc gcgatcgcgg
     7681 cgacgccggc ggcgaacagg agcgcgacaa gtaccgctga tcgtgacgct tgctggaaca
     7741 cgactgctcc cgctggcgcg tgggccgcct gtacgccgaa taaagagcct cgctgttgtt
     7801 gtgatatatg aagcgggcgc ggcgggcaag ctgcatccgg accaacaggg atccccggaa
     7861 tccagggatc gagcgatcgc gggacccgtg gcccaccgtt accgggacgg agcagattcc
     7921 ggctgcgagg ttggcactgg cagttcgccc agcagcgcct cccacgtctc gagcttgctc
     7981 cagcccaata tgcgtttgcc gtccaagaag acgacgggca ccgccgatat gcccagcttg
     8041 ttcgccagct cgatgtcgga gcggacgcga tcggcgatga ccggggattc cagcgcttgc
     8101 tggaaggccg ccccgtccag gttgagttcg gctgcccagg attcaaatag ctcaagctga
     8161 gcggggcttt gttgcggcag cggaactttg ggcaggaggt tttggcgctc ccaaagcaag
     8221 gcgcgcatgt tgagatacgc ttcgaggccg ccgacggcgc gggctgcttc ggccgccgca
     8281 acggcccggc aggcggagaa gtgcccgccg gtgcggaagc gcgggttagg attgcaggct
     8341 gtgtcttgcg ggtagtagcg gaacgcgatg cggatttggc ccgcgtattt ccgtgcgact
     8401 tccatcagca tctcgtgcga ctgcttgcag acggtgcatt gaaaatcgcc gaagaccacg
     8461 accgtgtgcg cggcgtcggg gttgccggcg aacacttcgt ccgcgtagag cgggatatcg
     8521 gccgccgcct ggcgctggaa gtcccagcgg acgaactccg ggtcgcccac gattctcgtg
     8581 tactcggcca tccgttcgcg cgttattgac ccgaccactt gaacaaacac aacaagcaag
     8641 tgcaccagca gcaggaggac cccggccaaa atgaccgcca gcgccaggcg ggcggtcggg
     8701 tgcggcagga cctcgcggcg cgggcggcgc cacggccagg cgatcaggct tagcaggaca
     8761 aggccgccgt tgagagtatg ggccgcgatg cagatcggac accaacggtt caggacctgt
     8821 gccatgacct ggattgctga aacggaataa atgattccgc cgatcagcac caaagccgga
     8881 acaagaaacc acttgcgacg ttcgtaagtc ggcggcccga tgaacaagta ccacaccagc
     8941 agcgcggcaa aataggccat gccgaaggtc gccgtgggta tgcgcagcga gccggcgttg
     9001 gggccgagcg cgaggtaggc ctggggcgag gtgagcacgg cgccgcagtt gtcgaggccg
     9061 ctttcgtccg ggccgccgca gaacttggtc agcagcgggt ccagtccccc cgtctgagcg
     9121 gaaacgccga aagcgcggta tgagacgtac cagccagcgg ccgcgataac gaccgcggcc
     9181 caccgcaacc aaggcttagg ttgcacgaag gagacgggct ggcagaccgg gatagtaagc
     9241 tgctctgcgg cggacaagga catttcgtgc ctcactggcg tcagtttggg ccggtcgcgt
     9301 ccggcctttg ccgcggcgcc tcacgctgcg gccgcttttg aaggacggca cagtatacgg
     9361 gctcataagc gtcgccggtg ctgcgccaga gttcgacgag atcggggtcg gcggtcaggg
     9421 cggatagggg caggcggtgc cggggcgacg cggcatggcg cgcgtcccag atcagcacgt
     9481 ccccggctcg cgacgattcc cagcgctggg ccaggtccgg ctcgaccggc ggccggacgc
     9541 ggcccacaaa ctcgtctatc cagggcgaag cggaaaagat tgccgccccc gtcgtttgat
     9601 ggtccgaggg cgagccagtc gctgcgcgag cgatttctgc ggccgtccga tgggccttct
     9661 cgcggtcggt cagttgcagc ggggcgcatt ggcgaaacgg gcggggaata gaactgacta
     9721 ccaacggctg aagcaatgcg gctgccaggg cggttatcgg caggacggtc aaggccagcg
     9781 tcggccgcgc catcttttgc tgtgaagaaa gcacgaggac tgtgaagaac agcagcatca
     9841 gcataagcgc tgccgcgtgc agaaccggaa ccgtccattc gaggtggttg ataatgagat
     9901 cggcgcggag gcgatgcagc tccaggccgg cggcgcccca gaagacaatg acggcggccg
     9961 caccgatcgc gttcgcgttg agcaacggga ggttttgcgg gccggccgcc caacgccgcc
    10021 aggcccgccg gtactcggat agtgccgccg ccgcggaaac cgccacgacc ggtccgaccg
    10081 gcacgagaaa ccgcgcgtag ccgccgctac cgaaaagccc aaagccgtac atgaccgaat
    10141 gtgccgccac gtatgccgcc gcgctgctga cgaacagcaa cccgccgcgc cgccgtgcca
    10201 gaaccggcgc gccgatgatc gccagcagca gcgttccaaa accggccgcg agcacccaca
    10261 gaacgatcat ggtgagcagg ctgccctggc cgtactcgct cgtcggatgc ggctgaagca
    10321 gcagcttcag cggccattcg cgcaggaacg cgaatgcgag caggttgtgc agcagcggcg
    10381 cccagaagac ccagagccac tcgcgggccg gtcgggcacg gtaaagcagc gccgccagcc
    10441 acatgacgag gaacagggcg gcctcgtggc gggtgattgc acagagcgag atcgccgcgg
    10501 cgctccaggc gaagcggccc cggacgtaca gccagcatgc cagactcagg taaaaagcca
    10561 aagccgtctc ggtgagcgtc gtgtagctga gcgtgaagga cagcggctga atccagagga
    10621 atagcgggac gaaggccgcc aggcgcacgc gcagttggac cgcgaccaga taagcgagcc
    10681 aagcgctcgc ggccgtaagc agcccgctga acagcctggc cggcagccaa cccaggccgg
    10741 cagggagggc gtacaaggcg gtgaatcccg ggcggcccca gtcgtcaagg aggtaggcgg
    10801 gatgctgcca cgcccagcgc gcgatctgca ggtgcgtgag atcgtcctca tgatggacgc
    10861 cggtgctgcg gacggccatg acggccgaga cgatcaggcc ggccaacaca aagctgatgg
    10921 cgaggctcca gccggcaggc cgtgctcgtc ccccggccag cgttggtttt gttgaagcgg
    10981 cctcgaaaga ccccgcgtgg cggtggcgca tgggagcagc agaccagaat gcggcggctc
    11041 tggcaagtcc cggcaataca ggtaccgccc cgaacgcgcc cgccccgtcg ccgggccttg
    11101 atgacgcccg ccccagggcc ccaaattacg gttgtcgtcg tggcggcgct gttttcgcga
    11161 ccgcggtagc cggttaccca ttcaccgagg gcgtttttct ccgaggggtc catattttcc
    11221 gacgggtcca gaggggggcg gcgttggacc acctcccgac tgtccgtcaa gattttttgc
    11281 gggccccctt ggcgcatttc gaagcagaat ccgttatgct atgtgcttcg cggtgacccg
    11341 gcgcgagtta tcgggttcta cctgaacgcc ggccgctatt tagaggagct ccggcatgcc
    11401 ctttgaggac aagacaatcg tttgcgccga ctgcggccag gaattcgtgc actctgccga
    11461 tgaccaggcc cgttatgccg agcgcggctt tactaatgaa ccccgccgct gccgcgcctg
    11521 ccgcgataag cggaagaccg aaggcggccg gggtggcggt ggaggtggag gcggcgaacg
    11581 tggcgggggt cgctcctcgt ccggcgggcc gcgcggtgga gggcgcggca tgcagcagtc
    11641 gcgggaatcc ttcgaggtcg tgtgctccga atgcgggacc accacgaccg taccgttcaa
    11701 gcccagcggc gaccgcccgg tttactgccg ggactgcttc cagaagcgtc gcggcggttc
    11761 gcgcagcggc cgcggccgct gacatcgggt aagccgcaga cgcgcggcaa agccgagcga
    11821 cgcggctgaa cgccgaccga cgcggcgaaa gccgagcggc ggcgaaggct gagcgacgtt
    11881 gaagcggagc cggaccgtgt cggcccggaa tgggggagtg tgcgctcgcg ttttctgcaa
    11941 ctgggccgcg gtgcaacttg cccgcggttt ggccgagctc cggagcgatg cgcctgagct
    12001 tgcatcgcgc ctaagacttt gcatgcgcct gccgatcagt gcgggcggaa atgttccggg
    12061 gcgacatgcc ccggctctgc tcctcagagc actcccgcga acatcagcag tagcgccagt
    12121 gcttccgccg cgctaatcac cagcgccgtc acgccccaac ggaattggcc cggcgttcgt
    12181 acggcagcga tagaaaccag cgccgcaatc ggtacgccca tcaacatcat gctcgaaccg
    12241 agcacataga gcatgtgccc tcagcaaaag aacatcgtga gcacataccc gatgatcggc
    12301 acgcctgcga aaaccaaagc cgcttttccg cccatgtact ctccagacgc ccccgggccc
    12361 accttaagtg tatcggtcgc ggaacggctt gccacaggat cagacggtca ctgccgccgg
    12421 gcacgtccgt tcgggcgctc tccggaacag gaaacttcac agtacgtgaa gttcatgtag
    12481 cattagcacc acaaaggccg ccacgtccgc cacgctaatg gccaaagcca ccctgcccca
    12541 ccgcctccgc atacacaccg caacggtgga tactaacagt gcagccggta tccccagcag
    12601 aatcatgccc agaccgagcg tgtccagcgg gggcactcag cagtaggtca gtccgaggac
    12661 aaagccgacg atcgggactg cactgatgaa caaagcgatt ttcccgacca tgagtaacct
    12721 ccaaccacaa tcgcaagcgg gtgcctgacc ggcctcaggc cgccttttcc gccatttgcg
    12781 gcagcgaact cataatgtcc tgcacaatga tgtcggtact cagcccctcc gcctcgcctt
    12841 cgaagttcag cagcaggcga tgccgcaggg cgtgcggagc ggcctttttc acgtcttcga
    12901 agccgacgtt gtaccgcccg tcgagcagcg catatatctt ggcagccagc gtgagcgcct
    12961 gcgcaccgcg cgggctggaa ccccagcgca cgtatttgtt gacgctgggg gtcgcgaagg
    13021 ccccgttggg gtgcgtggcc agcaccatac ggatcacgta atcctgcacg tgcggcgcga
    13081 tgacgacgcg cttcaccagg tcctgcccgg caatgatctg ctcgcccgtg agcacgtgct
    13141 gaacctcggg cttgtagccg gtggtcgtac gatccaggat cgtcttcagc tcctggcggt
    13201 cggaatagcc cacaatcagc ttgaagaaga agcggtcgag ctgcgcctcg ggcagcgggt
    13261 atgtgccttc ctgctcaatc gggttctgcg tggccagcac gaagaacggc tccttcaacg
    13321 tgtacttcgt gccgccggag gtgacggtgt gctcctgcat ggcctcgagc agtgcggatt
    13381 gcgtcttcgg cgtggcgcgg ttgatttcat ccgccaggac gatctgggcg aacagcggcc
    13441 cgcgcgtgaa ctggaactgg cggcggccgg tctgcgggtc ttcggtgatg atgttcgtgc
    13501 cgatgatgtc ggcgggcatc aagtccggcg tgaactggat gcgggcgaag tccaaactga
    13561 gggcctcggc cagtgtgcgg atgagcagcg tcttgcccaa ccccggcacg ccctccagca
    13621 gcgcatgccc gccggcgaac aggcatacga gcaccgcttc gaccacgtca ttgtggccga
    13681 cgatcatctt ctggatttcc gctttcaggg ctgcgaattg ggtgcgaaac tcggccacct
    13741 gctgctcaac ctgaccggcg tcacgaacgg ccataggcgt ctcccgtgga aaaaggctta
    13801 accgcagagg acgcggagga tgctgaggca ggagctctgc ctctgcgtgc tccacgtcct
    13861 ctgcaagagg atctacgcaa atcgcgggaa aaattctacg caaaacgcgg gaaaaatgct
    13921 aagctttatg agttcgcctg tcgcgatcat ccagccagga tttgatctcg gggtagacct
    13981 gcgcggtcgc gatcggggcg gcctcgaatt cgccgtgtgc gatttccggg tacaggcgga
    14041 acgtcttatc gcgtgcctga atgcgctcaa agaacacctc gcgcgtgacg gccgcgttgg
    14101 cgatccggtc gcgcccgcct gctactacca gcgtgggcac gctcacgttc gcataattat
    14161 ctgagtagac gtggtccggg ctgcccaagg tgctgacaaa cgctttcgcg cgcacggact
    14221 ttgccatctg cgccagcacg ccggccttca tgtggtccac gatgtaacgc cgcccctgaa
    14281 gaagcacctc ggcccggtag ttcgtggcgc cggtgaaccg gccggccacc cccaacaagc
    14341 cctgcaccag tttgcattgc gcctctgcca gcgtgtccgc cagagccgga ggtagttttg
    14401 tccaccacga ctggcgccct tcgccgcgca tccagcccag cggcacgtgc cccgcttcgg
    14461 caatcagggc ttgcagccac gcccagcggg ccagcaactc aaacgggtaa ttggcgtccg
    14521 cgtccgtgcg caggaaatcg cgcagcagcg ccttccagtt cacgcggccg ctcgcgtcac
    14581 acgccgaact gggccagcac agcgccgccg gaaattcgac gaatacggcg ccggctagct
    14641 gctcggcccg ccgcgcggcc agttccgcat ccgcgacgat gtgtgcttcg gcgccgccgc
    14701 caatgagctg cgccccttgc gcgaagccgg ccagcaccat ggcccccatg ctcgcgccaa
    14761 caacaaacgg ccgtcgcccg gtcgcggcca gcacgtgctc cacgacggcc ggcatgtcga
    14821 agacgatgaa atgatccacc gaccagtcgt ccagcgccgg cggcggcaag ctgagcatgt
    14881 gcggcgcgcc atgaccgcgg aagttcacca gccaaaggtc ataaccggcc gcgctcagga
    14941 tgcttgcgag actgtgatat tcataatccg ggccagtaat gctgggcagg tcccacaagt
    15001 ccgcgttgac ccccagaccg tgcagaaaga tgaccggcgc gccgcgacca gggcggcgtt
    15061 ttacagccag gcgtgcaccg tcggacgtag tgatgtattc gatctctcgc tggccgttac
    15121 cgggctcgtt catgtcgatt ccgctctgga ggcacaaggt tggccgccaa tttcgccgcc
    15181 tcgacgacaa tcagcggcgc gattgccagc ccggcgatca gcaaccaggc cggcccgcca
    15241 acgccgctca cctggaaaat cgggcgcagc gcgggaatcg tcaacgccgc gagctgcaac
    15301 agccccgaaa atgcaatcgc cgccagtagc cagggattgc tgagcaggcc caggcgcggc
    15361 agcgtgtacc ggtcgctacg gcagcccagc gcgaaggcga gctgcgcgta cgcgagcgtg
    15421 ccaaacgtga cggtgcgggc gactgccagg ttttggggat cgccggcata cgtcaggtgg
    15481 aaagcgagcg cgccggcggc ggcgatcagc ccgccatgaa agaggatcag cccgccgcgc
    15541 ctgagcgaaa tcaccgcctc gcgcggcggc cgcggcgggc gcgacatgat gtctggttcc
    15601 ggcggctcca gccccaacgc cagcgccgga aaagcatccg taaccaggtt gatccacaga
    15661 atctgaatcg gtaccagcgg cgccggccag cccagcagcg cgccaaaaaa catcagcagc
    15721 acttcgcccg cgttggccga aagcaggtaa tgcacgaact tgcggatgtt gtcgtagata
    15781 gtgcggccct gctcgacggc attcacgatt gtggcgaagt tgtcgtccgt cagaaccatg
    15841 tctgacgctt ccttggtg
//
LOCUS       DAOSYS010000057        15788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3103_length_15788_cov_5.471112, whole genome shotgun sequence.
ACCESSION   DAOSYS010000057 DAOSYS010000000
VERSION     DAOSYS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3103_length_15788_cov_5.471112"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            510..1004
                     /locus_tag="PLU99_05255"
     CDS             510..1004
                     /locus_tag="PLU99_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HOO16499.1"
                     /translation="MKFILIDRISVLERGQRIVAHKALSLAEEYLADHFPAFPVIPGV
                     LMLEAMVQASAWLVRDALGFAPALVLLKEARNVTYKSFVAPGEVLTIESVCKELADGQ
                     SEFAALGRVGDREIVKARLVLRHLNLADSDPALAEIDTKLREHARTLFGLMRVESLAG
                     AVTV"
     gene            1036..1437
                     /locus_tag="PLU99_05260"
     CDS             1036..1437
                     /locus_tag="PLU99_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOO16500.1"
                     /translation="MPNVNRDEIFEKVRQTLVDALSVEEDEVTESATLTGDLGAESID
                     FLDIVFRLEKAFNIKIPQEELIPREILSNAAYVENRRLNATGLAALKSAMPHADLSAF
                     EKDPDIDKISDVFTVGTIINFVQAKLELQAA"
     assembly_gap    1529..1628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1629..2060
                     /locus_tag="PLU99_05265"
     CDS             <1629..2060
                     /locus_tag="PLU99_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HOO16501.1"
                     /translation="AVKNVSLAEDHLHDHFPGYPVMPASLMIEGMAQTAGILVGEARQ
                     FAEKVILAKVKRATFHEIVRPGQQIRHEAVIEQLSDSAASTSGRIYAGERLIGEVDLV
                     FSHIDQNLSGLNFPEENFVFTEEFMALLKTYRGQAAEGVKL"
     gene            2057..3397
                     /locus_tag="PLU99_05270"
     CDS             2057..3397
                     /locus_tag="PLU99_05270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOO16502.1"
                     /translation="MSPQRVVITGLGPVTPIGVGVAAFWNGLLEGRNGIRRIQAFDPA
                     RFASQMGGEVLGLQIADYVPKSYRKASKIMARDIVLAVAAAHQAVSDAGLRTRCLLDR
                     GDVQGEPNFDPARFGANIGAGLICADLHELAEALHSASESGVFDIRKWGAEGMNNLTP
                     LWLLKFLPNMLACHVTIVHDAEGPSNTLTCGEASSHLAIGEACRGIARGVADVCICGG
                     AESKANPMALLRQSLGGRLSQRNDAPESACRPFALGRDGTVVSEGGGLVIIEELEHAR
                     KRGARIYAELVGFGAANDVFQAGQPGQLSPNGRGLALAARKALKDAGISADQVDLVTA
                     SGYGIPHQDRAEAAGIREVLGPRAADVPVMSIKGGLGNNGAGSGAIEFIGAVMAVHSG
                     RIPPAINSQPPDPACALKLVTGGPVDAKIEYVLSTGFALTGGQCAALVIKRWHE"
     gene            3434..4729
                     /locus_tag="PLU99_05275"
     CDS             3434..4729
                     /locus_tag="PLU99_05275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOO16503.1"
                     /translation="MNRRVVITGMGWVTPLGCELERVWQRLLNGESGIAPTTIFDAGS
                     YPTQFSAEVKDFDFQATLGEHSEDHRTASRNARFALGAAEMAWKHAGLDRSESGATPE
                     MTGVYLGGGEGPIDFENFVAAAVAGWDPQARQLDAVRWAEVAYERLDAIREFEQDPNC
                     AAHHIACRFHAEGPNLNTLTACAASTQAIGEATMIIRRGDADVMITGGAHSMIHPLGV
                     TGFNRLTALSTRNDSCATASRPFDATRDGFVLGEGAGILVIESLENAQRRGARILAEI
                     IGYGSTADAFRVTDMHETGRGPIAAMRAALADAKVAPQDIHYISAHGTGTEENDKIES
                     LAIGAVFGDLAKKVPISSVKSMLGHLIAGAGAVELITCVLAIRDGIIPPTANYSTPDP
                     NCPLDYVPNKPRKATVRTCLSNSFGFGGQNDTLIVRAFE"
     gene            4900..5634
                     /locus_tag="PLU99_05280"
     CDS             4900..5634
                     /locus_tag="PLU99_05280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO16504.1"
                     /translation="MPSRKAALPARATRLSRLAAAWRLLVLVIALGAFYRAGPALCAW
                     WFAPHQPDRFDQTAFYFSLLLVLAALLTLGLERLRDLPSLFRSRDKLPAQLIWAISAV
                     ACIYAFDVLVAVPYWLWFRPTGEVGSPTAKTLFAAATQNRAALVLLVTAIGAAAEELF
                     FRGLLLRYLTDLFRAPKLAILTSAAFFGLAHLGAGYFNALIALWMGIVLGAIYARRGG
                     LLTVVAAHFLFNASNLLLVPAIWHAE"
     gene            6021..7166
                     /locus_tag="PLU99_05285"
     CDS             6021..7166
                     /locus_tag="PLU99_05285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOO16505.1"
                     /translation="MLIKLGALGDVIRTAALLPGLKAAWPESHITWVSRPAGVRMLAN
                     HPLIDRLLPFDAESLCHLECEQFDLCLSLDKEPAPAALAMRVRAAQRRGIGLSRFGTV
                     FPLNSQCAAYFRLGLDDEFKFRCNEKAYQELIYQAVGLSYSGERYQLYPSAAHCRWAG
                     RRWQAHQVEPHEKVIGLNTGAGHAFANKIWRAEKFTRLARELVRKHGWRVALLGGPRE
                     AQTNKAIAAACAGLVGRGGGPAVVDVCAAGSEGGLGAGRHAQDAPSSPALHELEFAAL
                     VERCNVVVTGDTMAMHVAIALGVPAVVLFGPTCPQEIDLYGCGTKICTRLPCAPCYRR
                     ACDKSPNCMDDIGVPEVLAAVEHWAEVTPETPASVTAVPSPAMCCSQ"
     gene            7579..8661
                     /locus_tag="PLU99_05290"
     CDS             7579..8661
                     /locus_tag="PLU99_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOO16506.1"
                     /translation="MDPHPQPQPQVRPLEKLGQGWTDQLQPLEPLDVMKTATFDQMLR
                     AMSRTAFSGRNLGEAADVLYQMATDDKCFVVMTLSGAMTMAKMGLVITEMIDQGMVNA
                     IVSTGALMCHGLVEQTGHTHFRYDHRWSDEQLYEAGYARVYDTLELETNLAAAEGIIR
                     SVLEQMPPEQPFGSHELHRRLGEHLRQTTSGRGILQSAAANDVPVYVPSFTDSELGID
                     LFLFNLRRRLAGQPQLQLDLLRDLDDYLQRALRAERIGIFTIGGGVPRNWAQQVAPMA
                     EILHRNTNGAVGGLMRYQYGVRICPEPTHWGGLSGCTYSEGVSWGKFVPESEGGRYAE
                     VYADATVAWPILVRAVLERMGRVGKG"
     gene            8874..9179
                     /locus_tag="PLU99_05295"
     CDS             8874..9179
                     /locus_tag="PLU99_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HOO16507.1"
                     /translation="MAAKTVKCAKLGQELPGIDETTPNGRQALKMCLLIGGPALRQRV
                     LDTISAQAWEMWTDHMRMIINEFRLDPTSDESNQVLGQFMEDFFFGPQREVPGYVPQ"
     gene            9303..10241
                     /gene="ispH"
                     /locus_tag="PLU99_05300"
                     /gene_synonym="lytB"
     CDS             9303..10241
                     /gene="ispH"
                     /locus_tag="PLU99_05300"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOO16508.1"
                     /translation="MGESGPAQAIVAKPRGFCAGVERAIRAVETALERFGPPIYVRKE
                     IVHNLHVVQRLRERGAAFVDELDQVPSGAVIILSAHGSPPEVYREAERRGLRLVDATC
                     PLVTKVHHEVRRYAERGYRIILIGHAGHDEVVGTMGQAPQCTDLIKRLEDIDRLNLDP
                     REKGVILTQTTLSEDDTREIVQALRRRFPQLELPGRNDICYATQNRQNAVKRIADRID
                     LLLVVGSRNSSNSQRLTEVARARGVESYLIDSPDDIDPAWLVGKRCVGISSGASAPED
                     VVQAVLVWLRKAGVSHVEEIEAPDEKVVFTVPKLSS"
     gene            complement(10442..12055)
                     /locus_tag="PLU99_05305"
     CDS             complement(10442..12055)
                     /locus_tag="PLU99_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOO16509.1"
                     /translation="MQRLRDKIANFPKSPGVYLMKDREGVVLYIGKARDLRARVSSYF
                     QNSTDLLASRGPEIAHMATLVEDIDFLESETEVDALLAENRLIKDIQPRYNERQKDDK
                     SFPYLEITTGDDFPAVYVTRKPRLKGVKLYGPFIAASGLREAVNALQKVFKFRTCELD
                     IRDDDPKRRFFRPCLLHAIGQCTAPCAAYISKEAYAADIRRLMRFLNSKRSVVLRQMT
                     KEMEAAAAERRYEDAAVLRDRIKAIQSLALSGDPDEDVQPEVFHIDPRAGLDRLQRVL
                     GLPDAIRIIEGIDIATLHGEASVGSLVCFIDGKPYKAGYRRFRIKHVEGVDDYAMIRE
                     VIVRRYRHAAIAEELFPDVILIDGGLGQLHAALEAFNSMYEAVEVEGGRAMRPPMVIA
                     LAKREELLYVQARREPLRMARNNEALRLLQQIRDEAHRFGQHYHHLLRRKKAFDEDVA
                     AGRRPPRASRRKSAPEPPASPHSAGPASPSPAEPSQQRDASAEPRPQEPQSGPPRIPP
                     QSPFKRPPKLKPGETHLDPSESQIPPTED"
     gene            12351..13622
                     /locus_tag="PLU99_05310"
     CDS             12351..13622
                     /locus_tag="PLU99_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16510.1"
                     /translation="MYPRFITRGLLTALGDTPIAVLHGPRQAGKSTLARHIAEHDHPA
                     TYFTFDDPTVLATVSGDPTGFLANTPGCLVLDEIQRVPELILPIKAEVDRDRRPGRFL
                     LTGSANLMQLPKLADAFVGRMEIHTLLPLSQGEIEGMREGFIDALFATKAPPWPASGT
                     AELGTLSRTDLARRILAGGYPEAIARPESERSRWFESYLSTVMLREIRDLANIEGLVD
                     LPRLLVAVAGRAGGITNYAELGRDVGLNQVTVKRYLTLLTATFLLRPVSPWFTNRITR
                     IVKSPKLYFGDAGLLAHVLGATADRFAADPKLAGVLLETFVATELLKQISWSHTKPSL
                     WHFRDHRGHEVDFVLEPRGARQIVGIEVKNKATLDTDDFRGLKVLAAAAGERFHRGVV
                     LYTGAQALPFGKQMYALPIAALWRLGAKRVG"
     gene            13732..>15788
                     /locus_tag="PLU99_05315"
     CDS             13732..>15788
                     /locus_tag="PLU99_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO16511.1"
                     /translation="MKPRDRKRPVLVNIKPSRQAESGQAPSRGKRSRQTQPGPSRRSW
                     IILLGACAFVLPALAVGIFLYVLPQRPSAKHPPAARPLLGNGRNVLLITVDTTRADYL
                     GCYGRPGGRTPNIDRLAQEGALFERCIASAPLTGPSHASILTGNHPFVHGVRRNSTVP
                     LASENISLAEVLKDSGYATAATVASFVLNRQFGFAQGFDYFHDVSQTSVDDPQSAERK
                     GDEISRDAISLLQTLAPAPFFLWVHYYDPHHPYETKRSPPFTPAEAYEDEIAFMDEQI
                     GLVLGELENLGLAQRTIVVLVGDHGEGLGEHGESQHAYLLYNSTQHVPLIVRCPEAVV
                     PGTSLAEVVRTIDVPPTILALLGLPPVEQTQGVSLAGCLRGEMAVPALEAYSESFEAH
                     AQFGVSRLRSLMDQEWKYILAPKPELYAIADSLAETNSVIAEHPDVAARLRERLRMIV
                     AESPPPARPDSDAAATLSDADRARLESLGYAGTAVRPDEVNGPELERFEPEGANPMDF
                     TAQFELTVQAGDLLQVRDYAAATPLLQQVLAILPDAPRPAGLVATALAAQGRPHEAIP
                     IYERVLKLIPEDTYLRGSYGIALMQVGRTQEAADNLRIVLRENPRSARVLHNLAIALT
                     QLGRLAEADQNLQLALEVEPQNPRVLHTLGILRARQNRLAEAEQYLVQALQIDPSFRQ
                     CQE"
BASE COUNT         2698 a         4919 c         5272 g         2799 t
ORIGIN      
        1 acacgcagcg cgctattccc gacccctcgc tggccgacaa gctcgcgcaa atctgttgga
       61 cgccggacat gcgtagcgcc ggccaccagg gcgatcacga cggcatgttc gaatacgaat
      121 ggaaccggcg ctacgtcgcg ccgcagctcg tggcgcgtca acagggtggg tgattgcccg
      181 aagcttgtaa gcgcgcggtg acggcgggtg ccatgcccaa gctgtaagcg cgggcatgcg
      241 ctcgttaacg gcgacaggtt agccgcgcca ggttaatggc gttacggcgt caggtgcggg
      301 gcaactggtg gcggcatgcc cgcgcccgca acgcgggctt gggcatggca cccaccatct
      361 tggcacccga taccttggcg cccgatgcgc gttccgcgct ctggaacgcg cgtccacttc
      421 ccgctaaaat ccgcccgcaa atgaaggtgg cggtgcgtgc acgccgtttt gagtccctcg
      481 gcgatcacga cttggcaggg tgagagcgcg tgaagtttat tctcattgac cggatcagcg
      541 tcctcgagcg cgggcagcgc atcgtcgcgc ataaggcgct ctcgctggcc gaggagtacc
      601 tggctgacca ttttccggca tttccggtca taccgggcgt gctaatgctg gaagccatgg
      661 tccaagcctc ggcgtggctg gtgcgcgacg ctctcggctt tgctcccgcc ctggtcctcc
      721 tgaaagaagc ccgcaacgtt acgtacaaga gcttcgtcgc ccccggcgag gtgctcacga
      781 tagaatccgt ctgcaaggaa ttggcggacg gtcaaagcga gttcgcggcg ctcggccggg
      841 tgggagaccg cgaaatcgtc aaagcgcgcc tggtgctgcg gcatttgaat ctggccgaca
      901 gcgacccggc tttagctgaa atcgatacaa aactgcgcga gcacgcgcga acgctgtttg
      961 gattgatgcg ggtcgaaagt ctcgccggcg cggtgactgt ttgattcgtt tattagcagt
     1021 ggcctggagt ctggaatgcc gaacgtaaac agggacgaaa ttttcgagaa agtccggcag
     1081 acgttggttg atgcgctgag cgtggaggaa gacgaggtca ccgagagcgc caccctcacc
     1141 ggcgatcttg gcgccgaatc gatcgatttc ctcgacatcg tcttccggct ggaaaaagcg
     1201 ttcaacatca agattccgca ggaagagctg attccgcggg aaatcctgag caacgcggcc
     1261 tacgtcgaga accgccgcct gaacgcgacc ggcctcgcgg cgctgaagag cgccatgccg
     1321 cacgccgact tgagcgcctt cgagaaagac ccggatatcg acaaaatctc ggatgtcttt
     1381 accgtcggca cgatcatcaa cttcgtgcag gccaagctcg aactccaggc ggcgtaagcc
     1441 actggcgagc ggttgcactg cttgaagctg cgctttcaag cagtggcaca cttgtcggcc
     1501 cgtggggggt ttgggctgat aatggcggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnngc ggtgaagaac gtctcgctcg ccgaggacca cctgcacgat cactttcccg
     1681 gctatccggt catgccggcc agcttgatga tcgaaggcat ggcccagacc gccgggattc
     1741 tcgtgggtga ggcgcggcag tttgccgaaa aggtgatcct ggcgaaggtg aaacgcgcga
     1801 ccttccacga aatcgtccgg ccgggtcagc agatccgcca cgaagccgtc atcgagcagc
     1861 ttagcgactc agccgcttcg accagcggcc gcatctacgc cggcgagcgt ttgatcggcg
     1921 aggtcgacct tgtcttctcg cacattgatc agaacctcag cgggctgaat ttccccgagg
     1981 agaactttgt gtttaccgag gaattcatgg ccctgctcaa gacttaccgc ggccaggcgg
     2041 ccgaaggtgt gaagctgtga gcccgcagcg cgtggttatt acgggactcg gtccggtcac
     2101 gcccattggc gttggggtgg ccgctttctg gaatggtctg ctagaaggcc gcaacggcat
     2161 tcgccgcatt caagccttcg atccggcccg tttcgcatcg cagatgggcg gcgaggtgct
     2221 cgggctgcaa atcgcggact acgtgccgaa gagctatcgc aaggccagca agatcatggc
     2281 ccgcgacatc gtgctggcgg tggccgcggc acaccaggcc gtcagcgacg ccggcctgcg
     2341 gacgcgctgc ctgctggatc gcggtgacgt ccagggcgag ccgaatttcg acccggcgcg
     2401 cttcggcgca aacatcggcg ctggcctaat ttgtgcggac ctgcacgaat tggccgaggc
     2461 cctgcacagc gcctccgaaa gcggcgtctt cgacattcgc aaatgggggg ccgagggcat
     2521 gaacaacctt acgccgctgt ggctgctgaa gttcctgccg aacatgctgg cctgccacgt
     2581 gaccatcgtt catgacgccg agggaccgag caacaccctg acttgcggcg aggccagcag
     2641 ccacctggca atcggcgagg cttgccgggg tatcgcccgc ggcgtggccg acgtgtgcat
     2701 ctgcggcggc gccgaaagca aggccaaccc gatggccctg ctgcgtcagt cgctcggcgg
     2761 gcggctcagt cagcgcaacg acgcgcccga gagcgcttgc cggcctttcg cgctggggcg
     2821 cgacggcacg gtggtttccg agggcggcgg cctggtcatc atcgaagaat tggagcacgc
     2881 gcgaaaacgc ggcgcgcgga tttatgctga gctcgtaggt ttcggcgccg ccaatgatgt
     2941 cttccaagcc ggccagccgg ggcaactttc gcccaacggc cgcggcttgg cactagcggc
     3001 ccgcaaggcc ttgaaggacg ccggcatcag cgcggaccaa gtggatctcg tgacggccag
     3061 cggctacggc attccgcacc aggatagggc cgaagccgcg ggcatccgcg aagtgctcgg
     3121 cccgcgcgcc gccgacgtgc cggtcatgag catcaagggc gggctgggta acaacggcgc
     3181 cggcagcggc gcgattgaat tcatcggggc ggttatggcc gtgcatagcg ggcgcatccc
     3241 gccggccatc aacagccagc cgccggatcc ggcgtgcgcc ctgaagctgg tcacgggcgg
     3301 cccggtcgat gcgaagatcg aatacgtcct cagcacgggt tttgcactga ccggcggcca
     3361 atgcgccgca ctggtcataa aacgctggca cgagtaaccg gctgatagcg atagctgaaa
     3421 gctgagagct tttatgaacc gccgagtagt tataaccgga atgggctggg tgacgcctct
     3481 aggctgcgag ctggagcgcg tctggcaacg cctgctgaac ggcgagagcg gcatcgcgcc
     3541 gacgaccatc ttcgacgccg gttcataccc cacgcagttc tcggccgaag tgaaggattt
     3601 tgacttccaa gccacgctcg gcgagcactc cgaagaccat cgcacggcca gccgcaacgc
     3661 gcgcttcgcg ctcggcgcgg cggaaatggc ctggaagcat gccgggcttg accggtcgga
     3721 atccggtgca acccccgaga tgaccggcgt ttacctgggc ggcggcgaag ggccgattga
     3781 cttcgagaac tttgtggccg cggccgtcgc cggctgggat ccgcaggccc ggcagctcga
     3841 cgcggtgcgt tgggccgagg tggcctatga gcggctcgac gcaattcgcg agttcgagca
     3901 ggatcccaat tgtgccgcgc accacatcgc ctgccgcttc cacgccgaag gcccgaacct
     3961 caacacgctg acggcttgcg ccgccagtac gcaggccatc ggcgaggcca ccatgatcat
     4021 ccgccgcggc gatgcggacg tgatgatcac gggcggcgcg cacagcatga tccatccgct
     4081 gggcgtgacc ggcttcaacc gcctgaccgc cctgtccacg cgtaacgact cgtgcgccac
     4141 cgcctcacgc ccgttcgacg ccacccgcga cggcttcgtg ctcggcgaag gggccggcat
     4201 cctcgtcatt gaatcgctcg aaaacgcgca gcgccgcggt gcacgcattc tggccgaaat
     4261 catcggctat ggctcaaccg ccgacgcctt ccgcgtgacc gacatgcacg agaccgggcg
     4321 cggcccgatc gccgccatgc gggccgccct ggcggatgcc aaggtggcgc cgcaggacat
     4381 tcactacatc tcggctcacg gcaccggcac ggaagagaac gacaagatcg agtcgctggc
     4441 catcggcgcg gtcttcggcg acttggccaa aaaagttccc ataagcagcg tgaaaagcat
     4501 gctcggccac ctgatcgccg gggccggcgc ggtcgagctg atcacctgcg tgctggccat
     4561 ccgcgacggc atcatcccgc cgaccgcgaa ctactcgacg ccggacccga actgcccgct
     4621 ggactacgtg ccgaacaagc cgcgcaaggc gacggtccgc acgtgcctgt cgaacagctt
     4681 cggcttcggc gggcagaacg atacgttgat cgtgcgggcg ttcgaatagg attgagggat
     4741 caagggatcg agggatctag ggatctagag gtggcgtgtc gcgcatctct tgatctttgg
     4801 atgccgcatt ccggggcggc acgtccccgg ctccagccgg gtgtcgtcat agggcgatag
     4861 cgatctgtgt cgcctgaaat tccaggactg tcggagtcag tgccttcgcg gaaagctgca
     4921 ctgccagcgc gcgcaacgcg tctgtcgcgt ctggccgcgg cttggcgcct gctcgttctc
     4981 gtaatcgcgc tgggcgcctt ttaccgggcc gggcctgcgc tctgcgcatg gtggttcgca
     5041 ccccaccagc cggaccgctt cgaccaaacc gccttctact tctcactact gttggtcctg
     5101 gctgcgcttc tgacactggg actggagcga ctgcgcgatt tgccgtcgct tttccgctcg
     5161 cgcgacaagc ttccggcgca actgatttgg gccatttcag ccgtggcctg catctacgcc
     5221 ttcgacgtgc tcgttgctgt gccgtactgg ctctggtttc gcccaaccgg cgaagtgggc
     5281 agccctaccg ccaagacgct ttttgcggcg gccacgcaga accgggccgc cctcgtgctg
     5341 ctcgtgaccg ccatcggcgc cgcggcggag gagctgttct tccgcggcct gctgctgcgt
     5401 tacctgaccg atctattccg cgcacccaag ctcgcgatat tgacctccgc cgcttttttt
     5461 ggcctagccc atctgggagc cggctacttc aacgcgctca tagcattgtg gatgggcatc
     5521 gttctgggcg ccatttacgc gcggcgcggg ggtctgctga ccgtggttgc ggcccatttc
     5581 ctgttcaacg cttcgaacct gctgctcgtg ccggcaattt ggcatgccga gtagtcaaaa
     5641 ccgcggatta ccagccccgc gggcgcgcct cggcggcacg gcaacccttg cgcccacgta
     5701 ggcgacgcgc cggagtgcca tgaattctgc gggctgccgc gccagttatg tggaccctgc
     5761 cggcgccact ccccatctat tgccgtcgca ctcaggcccg ccgccctttc gtccgagaag
     5821 acttgcatct ggagaacagc gtgccggacc cgcctgtctc tcatcctaat ccacaccccg
     5881 agctgcccgc ggccagtgtc gtcgagccgc ggttgcgtct ggactgccgg aactttcgcg
     5941 gggatcggcc atgtgcggcg ggggtgcaag gcgtctgccc aacgagctgt cctcagtgcc
     6001 gccccattga tgcgcgcatt ttgctgataa agctgggggc cttgggcgac gtgattcgga
     6061 cggcggctct gctgccgggc ttgaaggcgg cctggcccga aagtcatatc acctgggttt
     6121 cgcgtccggc cggggtgcgg atgctcgcga accacccgct catcgatcgc ctgctgccgt
     6181 ttgacgccga gagcttgtgc cacctggaat gcgagcagtt cgacctgtgc ctgagtttgg
     6241 acaaggagcc ggcccccgcg gcgctggcga tgcgggtgcg cgcggcccaa cgccgcggga
     6301 tcggcctgag ccggtttggc acggtgtttc cgctgaattc gcagtgcgcg gcgtatttcc
     6361 ggctcgggct ggatgacgag ttcaagttcc gctgcaacga gaaggcgtac caggaattga
     6421 tctaccaagc ggtcgggctg tcgtacagcg gcgagagata ccagctttac ccgagtgcgg
     6481 cgcactgccg ctgggccggg cggcgctggc aggcgcacca ggtcgagccg cacgagaagg
     6541 tgatcgggct caacaccggc gcgggccacg cgttcgccaa caagatttgg cgggctgaga
     6601 agtttacacg gctggcgcgc gagctggtcc gtaagcacgg ctggcgggtg gcgctgctcg
     6661 gtggcccgcg cgaagcacag accaataagg cgattgcagc ggcgtgtgcg ggattggtcg
     6721 ggcgtggcgg cggtcccgcg gtcgtggacg tctgtgctgc cggaagcgaa ggcggcctgg
     6781 gtgcggggcg ccatgcccaa gacgcgcctt cgtccccggc tctgcacgag ttggaattcg
     6841 cggcgctggt ggaacgctgc aacgtggtcg tgacgggcga caccatggcg atgcacgtgg
     6901 cgatcgcgct gggtgttccg gcggtagtgc tattcggccc cacgtgcccc caggagatcg
     6961 atctttacgg gtgcggcacg aagatctgca cgcgtttgcc gtgcgcgccg tgctaccggc
     7021 gagcgtgcga caagtcacct aactgcatgg acgacatcgg cgtcccggaa gtcctggccg
     7081 cggtcgaaca ttgggcggaa gtgacgccgg aaacaccagc gtccgtaacc gctgttccgt
     7141 cccccgctat gtgttgcagc cagtaattag cgggagatcg tcaagctatc gattggggga
     7201 ggtgggaatt gttgaccgcg tttcgccatt agagatgagg tcggcttgga gctgcggtgc
     7261 ggtattcaac cgctcgctgc gtgctgaccc gtattggtgg gtcgaaacag tcatttcggg
     7321 gcgacacgcc agagacgggg ggcaactgcg cggacggaag cttcctggcg gcattccggg
     7381 gcgacacgcc ccggctctgc tctcccctcg atcccttgac gcctcgatcc ctctctcttg
     7441 atcgctcgtg tccgtcgaac gaaccgcacg ggaccgtcgg cccacagcct gggcgcctgc
     7501 ccatgcttga caatccccgt ccgcggccta tggttctggc acgattgtcc ccttaaagcc
     7561 ggaacaccgg aggtcgtaat ggatccgcac ccgcagccgc agccgcaggt tcgcccgctg
     7621 gagaaactag gccagggctg gactgaccaa cttcagccgc tcgagccgct ggacgtgatg
     7681 aagaccgcca ccttcgacca gatgctgcgg gccatgtcgc gcaccgcctt tagtggccgc
     7741 aacctgggcg aggcggccga cgtgctgtac cagatggcca ccgacgacaa gtgcttcgtg
     7801 gtcatgacgc tctccggggc gatgaccatg gccaagatgg gcttggtcat cacggagatg
     7861 atcgaccagg ggatggtcaa cgcgatcgtc agcaccgggg ccctgatgtg ccacgggtta
     7921 gtcgagcaaa ccgggcatac gcacttccgc tacgaccacc gctggagcga tgagcagctt
     7981 tacgaggccg gctacgcgcg cgtctacgac acgctcgaac ttgagaccaa cctggcggcg
     8041 gccgagggca tcatccgcag cgtgctggag cagatgccgc cggagcagcc cttcggctcg
     8101 catgagctgc accgccggct gggcgagcac ctgaggcaga ccacgtccgg ccgcggcatc
     8161 ctgcaatccg cggcggcgaa cgacgtgccg gtatacgtgc cgtcgttcac cgacagcgag
     8221 ttggggattg acttgttcct gttcaatctg cgccggcggc tggcgggaca gccgcagctt
     8281 cagctcgatt tgctgcgcga tctggacgac tatttgcagc gggcgctgcg ggcggagcgc
     8341 atcggcattt tcacgatcgg cggcggcgtg ccgcgtaatt gggcccagca agtggcgccg
     8401 atggcggaga ttctgcaccg caacaccaac ggagccgtgg gcggcctgat gcgctaccag
     8461 tacggcgtgc ggatttgtcc ggagccgacg cactggggcg gcctgagcgg ctgcacctat
     8521 tccgaaggcg tttcgtgggg caaattcgta ccggagagcg agggggggcg atacgcggaa
     8581 gtctacgccg atgctacggt agcctggccg attctggtgc gcgcggttct ggagcggatg
     8641 gggcgcgtcg gcaagggctg agtgcaaaac atcaaagtgc aaaccgcacg agcagcgccg
     8701 ggtcgtatcg ccgccgggct ttttcccggc ttgcgccggc gctgcggccg aaaccggacc
     8761 gacttgccag gcggtttgcc ctcgggctcg ccattgtgcg gcatgtcgca tcgggatagg
     8821 tggacctaga atatggcaaa ccgcgcccgc tggggacgcg tctggagcac gatatggccg
     8881 cgaaaactgt gaagtgtgcc aaactggggc aggaactgcc gggcatcgac gaaaccacgc
     8941 cgaatggacg acaggcgctg aaaatgtgcc tgttgattgg cgggccggcg ttgcggcagc
     9001 gcgtgctcga tacgatctcc gcccaggcct gggagatgtg gaccgatcac atgcgcatga
     9061 tcatcaatga gttccggctc gacccgacct cggacgagtc gaaccaggtg ctgggccagt
     9121 tcatggaaga cttcttcttc gggccgcagc gcgaggtacc gggttacgtt ccgcagtgac
     9181 ggtattggac gagccgtcgc agccggagat cgtgccggcg cgccggccgc ctcgacgtgg
     9241 ccaacctcaa atcgctcata taataggttt gtgagctaag tttaggattt ccgactcctg
     9301 atatgggcga gagtggaccg gcgcaggcaa tcgtagcgaa accgcgcggc ttttgcgcgg
     9361 gcgtcgagcg cgccattcgc gcggtggaaa cggcgctgga gcgtttcggc ccgccgatct
     9421 acgtccgcaa ggagatcgtg cataacctgc acgtagtcca gcgcttacgc gaacgcggcg
     9481 cggccttcgt ggatgagctg gaccaggtgc cgtccggcgc cgtcattatc ctgtccgccc
     9541 acggttcgcc gcccgaagtc taccgcgagg ccgagcgccg cgggttgcgg ctggtggatg
     9601 ccacgtgccc gctggtaacc aaggtgcacc acgaggtgcg gcgttacgcc gagcgcggct
     9661 atcgcattat tctgatcggg catgcgggcc acgatgaagt ggtcggcacg atgggccagg
     9721 cgccgcagtg caccgatctg attaagcgac tggaggacat cgaccggcta aacctggacc
     9781 cgcgtgaaaa aggcgtcatt ctgacgcaga cgacgctcag cgaagatgac acgcgcgaaa
     9841 tcgtccaagc tctgcgacgg cgcttcccgc aactggaact gccgggcagg aacgacatct
     9901 gttacgcgac gcagaaccgc cagaacgccg tcaagcgcat cgcggaccgg atagatttgc
     9961 tgctcgtggt cggctcgcga aattcgtcga actcgcaacg cttaaccgag gtggcccgcg
    10021 cccgcggcgt ggagtcgtac ctcatcgaca gcccggacga catcgacccg gcctggctgg
    10081 tgggcaagcg ctgtgtcggc atttcctccg gcgccagcgc gccagaggac gtcgttcagg
    10141 cggtactcgt ttggctgcgc aaggccggcg tcagccacgt ggaagagatc gaggctccag
    10201 acgagaaagt cgttttcacg gtaccgaagt tgagttcgta gcggcgaggt gggggccaga
    10261 gcagagccgg ggggtgtggt ccgaagcgag agggatctag gtatctaggg atcaagggat
    10321 caagaggagc agagccgggg cgtgtcggcc cggaatgccg ccaggaatct cccgcccgcg
    10381 cgttacctgc tccgaccagc cctctcgccc ccacaatgtc gctaacagcc tctatgcaag
    10441 gtcaatcctc cgtcggaggg atttgggatt cgctgggatc aagatgcgtc tcgccgggct
    10501 tgagcttagg cggccgcttg aagggtgatt gcggtggaat tcgtggggga ccggattgcg
    10561 gctcttgtgg gcgcggctcg gcggacgcgt cgcgctgctg actgggctcc gcagggcttg
    10621 gactcgccgg tcctgcggag tgtggactcg cgggcggctc gggcgcggat ttgcggcgac
    10681 tggcacgcgg cgggcggcgg ccggcggcta catcttcgtc gaaggctttc ttgcggcgca
    10741 gcagatggtg gtagtgctgc ccgaagcggt gcgcctcgtc gcggatctgt tgcagtaggc
    10801 gtagggcctc gttgttgcgc gccatgcgca gcggctctct gcgggcctgc acgtagagca
    10861 actcctcacg cttggccagc gcgatcacca tcggcggccg catcgcccgg ccgccttcaa
    10921 cctcgacggc ttcatacatt gaattgaacg cctccaaagc cgcgtgaagc tggcccaagc
    10981 caccgtcaat caggatgacg tcggggaaca gttcctcggc gatggccgca tgccgataac
    11041 gccgcacgat cacctcgcgg atcatggcgt agtcgtcgac gccctcgacg tgcttgatgc
    11101 ggaagcggcg atatccggcc ttgtagggct tgccgtcgat gaagcacacc agcgagccga
    11161 ccgaggcctc gccgtgcagc gtggcaatgt cgatgccttc gatgatgcgg atggcgtcgg
    11221 gcaggccgag cacgcgttgc aggcggtcga gtccggcccg cggatcaatg tggaacacct
    11281 cgggctgcac gtcttcgtcg ggatcgccgg acaacgccag cgactggatg gccttgatgc
    11341 ggtcgcgcaa cacggccgcg tcctcgtagc gtcgctcggc cgcggcggct tccatctcct
    11401 ttgtcatctg gcgcaacacg acgctgcgtt tggaattgag aaagcgcatc aggcggcgga
    11461 tgtcggccgc ataggcttcc ttgcttatgt aggcggcgca cggggcggtg cattggccga
    11521 tggcgtgcag caggcacggg cggaagaagc ggcgtttggg gtcgtcgtcg cggatgtcca
    11581 gctcgcaggt gcggaacttg aagaccttct gcaaggcgtt gaccgcctcg cgcagcccgg
    11641 aagcggcgat gaaagggccg tagagcttga cacccttgag ccgcggcttg cgcgtgacgt
    11701 agaccgcggg gaagtcgtcg ccggtggtga tctccaggta gggaaagctc ttgtcgtcct
    11761 tctggcgttc gttgtagcgc ggctggatgt ccttgatgag gcggttttcg gctaggaggg
    11821 cgtcgacctc ggtctcggat tcgaggaagt cgatgtcctc gacgagcgtg gccatgtggg
    11881 cgatttcggg gccgcgcgag gcgagcaggt cggtcgagtt ctggaagtag ctggagacgc
    11941 gggcgcgcag gtcgcgggcc ttgccgatgt agagcaccac gccctcgcgg tccttcatga
    12001 ggtagacgcc gggcgatttg gggaagttgg cgatcttgtc gcgcaggcgc tgcacgcggg
    12061 ccgcgtcgcc atttggccgg cactccggcg gcgctgcggc cgggggcggt gggacggggt
    12121 cctgcggggg cggcgaaggg acgccttcgg acgctgacgg ggaattggac gagctatgtt
    12181 ccggcatacg tgggagatta tagggcaatt acgcgagcgc aagtcgaaga tggaggcggg
    12241 cctgacgaga gctgcgaagg ggggtcgcgg aaaatctaac agcggctgtt agattttcca
    12301 acagctcctg tttgattttc caacgcgatc tgttattttt gccgacagag atgtacccgc
    12361 gcttcataac tcgtggcctg ctgacggcgc tcggcgatac cccgatcgcc gtgctccacg
    12421 gcccgcgcca agccggcaag agcaccctgg cgcgccatat cgccgaacac gatcaccccg
    12481 ccacgtactt cactttcgac gaccccacag tcctggcgac cgtgagcggc gacccgaccg
    12541 gctttcttgc caacacgccg ggctgccttg tgcttgacga gattcaacgc gtgcctgaac
    12601 tcatccttcc catcaaggcc gaggtggatc gcgaccgtcg gcccggacgg tttctgctaa
    12661 ccggctcggc caacttgatg caattgccga agctcgccga tgcgtttgtt ggccggatgg
    12721 agatccacac tctgctgccg ctctctcagg gcgaaatcga gggaatgcgc gagggtttca
    12781 tcgacgcgct ctttgccacg aaggcgccgc cgtggccggc ttctgggacg gctgaactcg
    12841 gcacgctttc caggacggat ttggcacggc gcatcttggc cggcggttac cccgaagcca
    12901 tcgcgcgacc ggagtcggag cgatcgcgtt ggttcgagtc ctacctcagc acggttatgc
    12961 tgcgcgaaat tcgcgacttg gcgaatatcg agggactagt ggacctgccg cgcctgctgg
    13021 ttgccgtcgc cgggcgggcc gggggcatca caaactacgc cgaactcggg cgcgacgtgg
    13081 gcctgaacca ggtaaccgtc aagcgctatt tgacgctcct aaccgcgacc ttcctcctgc
    13141 gaccagtctc gccttggttt accaatcgca tcacgcggat cgtcaaaagt ccgaagctgt
    13201 acttcggcga tgccggcctg ctggcgcatg tactgggcgc gacagccgac cgcttcgctg
    13261 ctgaccctaa gctggccggc gtgctcctgg aaaccttcgt cgcgaccgag ttgctcaagc
    13321 aaatcagttg gagccacacg aagccgagcc tgtggcattt ccgcgaccat cgcggccacg
    13381 aggtcgattt cgtgctcgag ccgcgcggcg cccggcagat tgtcggcatc gaagtgaaaa
    13441 acaaggcgac gctggacacc gacgacttcc gcggcctgaa agtgctggcg gccgccgccg
    13501 gtgagcgctt ccaccgcggc gtagtgctgt acacgggcgc gcaggcgctg ccgttcggga
    13561 agcagatgta tgcgctgccg atcgcggcgt tgtggcggct gggagcgaag cgcgtcggct
    13621 aagggtcgcg gctcggctca agcattggca gagttgaacg ccgcgtactc ggtcgtatac
    13681 tgcgtttcgc acgcgctgct cagcgcaggc gagccgaatc ggagtccgcg catgaagccg
    13741 cgcgatcgca aacgccccgt gcttgttaac atcaagccgt cccggcaggc agagtcgggg
    13801 caggcgccgt cccggggaaa gcggtcccgg cagacgcagc ccgggccttc gcggcgttcg
    13861 tggatcatcc tgcttggggc atgcgcgttt gtcctgccgg cgcttgcggt cggcatcttc
    13921 ctttacgtgc taccccagcg gccctccgcc aagcaccccc ccgccgctcg gcccctgctt
    13981 ggcaacggcc ggaacgtgct gctgattacc gtggatacta cccgcgccga ttacctcggc
    14041 tgctatgggc gacccggcgg ccgcacgccc aacatcgacc gcctggcgca ggaaggcgcg
    14101 ctattcgagc gctgcattgc ttcggcgccg ctgaccggac cgtcccatgc cagcattctg
    14161 accgggaacc accccttcgt gcatggcgtg cggcgtaaca gtaccgttcc cctcgcaagc
    14221 gagaacatca gcctagccga ggtgctgaag gacagcggct atgccaccgc ggcaaccgtc
    14281 gcctcgttcg tgctcaaccg ccagttcggc ttcgcccagg gctttgacta ctttcacgac
    14341 gtaagccaga cttcggtcga cgacccgcaa tcggccgagc gcaaggggga cgaaatcagc
    14401 cgcgacgcga tatctttgct gcagacgctg gcgccggccc cgttctttct ctgggtccat
    14461 tactacgacc cgcaccaccc gtatgagacg aagcgcagcc cgccgttcac gccggcggag
    14521 gcttacgaag atgagatcgc ctttatggat gagcagatcg ggctggtgct gggcgagttg
    14581 gagaacctgg gcctcgcgca gcgcacgatc gtggtgttgg tcggcgatca tggcgaaggt
    14641 ctgggcgaac acggcgaatc gcagcatgcg tacctgctgt acaacagcac gcagcacgtg
    14701 ccgctaatcg tgcgctgccc ggaggcggtg gttccgggca cgtcgctcgc ggaagtcgtg
    14761 cgcacgatcg acgtgccgcc cacgatcctg gcgctgttgg gcttgccgcc tgtggaacag
    14821 acacagggcg taagcctggc cgggtgcttg cgcggtgaaa tggccgtgcc cgcgctggag
    14881 gcgtacagcg agtcgtttga agctcacgcg cagttcggcg tttcgcgctt gcgcagcctt
    14941 atggaccagg agtggaagta catcctggcc ccaaagcccg agttgtatgc gatcgccgac
    15001 agcctggccg aaacgaactc ggttatcgca gagcacccgg acgtcgcggc gcggctgcgt
    15061 gagcggctgc gcatgattgt cgctgaatcg ccgccgccgg cgcgccctga cagcgacgcc
    15121 gcggcgacgc tgagcgacgc ggatcgcgcg cgcctggagt cgctgggcta tgccgggacg
    15181 gccgttcgcc cggacgaagt gaacgggccg gagctggagc ggtttgagcc ggagggggcc
    15241 aatcccatgg actttaccgc acagttcgag ctgaccgttc aggccggaga cttgttgcag
    15301 gtgcgtgatt acgccgcggc gacgccattg cttcagcagg tgctcgcgat cttgcctgac
    15361 gcgccgcggc cagctgggct ggtggccacc gcgctggcgg cacagggccg tccccacgag
    15421 gcaatcccca tatacgagcg ggtgctgaag cttattcccg aggacacgta tcttcgcggc
    15481 tcgtacggca tcgccttgat gcaggtcggt cgcactcagg aggcggccga caacctgcgc
    15541 atcgtgctgc gtgagaaccc gcggagtgca cgggtgctgc acaacctggc gatcgcgcta
    15601 acgcagcttg ggcggctggc agaggcggat cagaatctgc agcttgcgct ggaggttgag
    15661 ccacagaacc cgcgcgtgtt gcacacgctc ggaattctcc gcgcgcggca gaaccgactg
    15721 gcggaagcgg agcagtacct ggtgcaggcg ctgcaaatcg atccttcgtt tcgacagtgt
    15781 caggaaga
//
LOCUS       DAOSYS010000058        15556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3176_length_15556_cov_5.930327, whole genome shotgun sequence.
ACCESSION   DAOSYS010000058 DAOSYS010000000
VERSION     DAOSYS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15556
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_15556_cov_5.930327"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2923)
                     /locus_tag="PLU99_05320"
     CDS             complement(<1..2923)
                     /locus_tag="PLU99_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO16512.1"
                     /translation="MQNVRLRRSLTDYGLAVGATLLACAAHLVMGRFAGELPPYIFFY
                     PALMIAALLGGLGPGLLATGLAALLAGWLILPLEHDRTLHADVNLALFFGMGVFVSVV
                     AGLYRRARDRAVRAERELSVQADLARAAEEVRASQHMLREVLDAVPVRVFWKDLNCIY
                     LGCNLHFARDAGFDSSEQVIGKRDCDLQRRWQPEICRVDDELVMQTGQAILNYERPVT
                     GPDGCRRWLRTSKVPLRAPDGKVIGVLGTYEDVTARKEAGEALAASEQRLRLATQSGQ
                     IGVQEIDLATGVACLDELTARILGIEPGERLTIDRFVREFMHPEDAANLYPQFLKWLE
                     SNEDHWGAEYRIVRPDGEERWIQDDYLAIRDESGKPVRAVGASSDITRHKRAEQALRQ
                     SQEMLRLVLDTVPVRVFWKDVNGVFLGCNQHFARDAGLESPDQIIGRDDYAMGWRDQA
                     RLYRADDQAVMESGQAKLGYEEPQTAPDGQLLWLRTSKIPLRGPDGKVFGVLGTYEDI
                     TDRKRAELTLRESEERFKALAAATFEGIAIEEGGRLVDANEQFLNMLGYSRDELIGME
                     VEALVPPEDRELVRRTVVAARQAQIEHTLLRKSGSRVTAEVRVTPVTYQNRVVRFAAV
                     RDITEQRRAEKALAAAKQGAERAMAIAEAASHAKDQFLAVLSHELRTPLTPVLGAVSM
                     LVRDDRLGAELRDKLELVLRNVKLEARLIDDLLDVTRIARGKLEMHRHPVQLGDVIRQ
                     AVEVCMPEIQSRRLELCMDLGEAGICTIDADATRLQQVFWNLLSNAIKFTPTGGRIGI
                     RCRRADGRLITEVSDSGIGIEPDVLPRLFTAFEQAERDSARQFGGLGLGLAISKALVE
                     MHGGTIAAGSPGRGRGATFSVTLPVGADTVGGAQAARPARPSAKEKRGLQILLVEDHG
                     DTARIISQLLRAAGHCVTAVGTVSAALHEAGKRKFDLLISDLGLPDGNGIELLRRLEA
                     AG"
     gene            complement(3033..5360)
                     /locus_tag="PLU99_05325"
     CDS             complement(3033..5360)
                     /locus_tag="PLU99_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOO16513.1"
                     /translation="MTEPLDTSTVDTWAIVETDFDVRNNRHYEALMAIGSGPLQQRAS
                     LEGGLSDDPQDREYLHLPQGAGPPAGRRPHNSTSSKSRFGTFLPGVTGPHPTCGDEMI
                     NLPAIHGLTLYAGHERLDMERSRIEGYRRRLDLRTGRLSREFTWHTQRGGAVAVRFER
                     FISAARRHVMALRCHLRHLSGPAVELRMVGTLDADVRTNGFDHFEHIALTGEHEPITI
                     EVRTNGGDTVAAAALMTCEPGVVWTVETEPRWAGIGGMRLLEPGQQVTLCKYAVLISS
                     RHVQGRPLDAARNLVWSAAGLGYERLAAESDAVWQRRWEQTDVVIEGDPRSQLALRCG
                     LYHLMRAAGDGDTRLAIDPCAATNEACCGRFTWDTDVFMLPAFLYSRPEVGRTLAGFR
                     LRSLPGARVNARRYGYSGARYAWESDPQGQEQCPAWQHADHDVHVSADVAYGLWHAYL
                     ANTSDVKFLAGVAEALTEIARFWLQRVTYNAARGEYELLMVTGPDQYTPFSRNNAYTN
                     RLVACALNLAVTAWRELESRDRAAAVELSARLALQASELEQFTETAGKLRFPYDEARK
                     LVVQCDGFFEQEPFELDRHWTDRARPLAASVPQERLFRSQVIKQADALQLIAVFPQEF
                     AVEQMQAAFDTYAPLTAHDSSVSRGMFALVAAWLGRTDEALRLWGESASRNLDPPGAA
                     EGVAAAAAGLNWQVVVFGFAGLRTLMQSQVLWIDPHLPRNWTALRFPFVWRGQPLFIT
                     IEQGRIRVEHRGDRPVDAQILGRPVRLEPGRRADF"
     gene            complement(5619..8684)
                     /locus_tag="PLU99_05330"
     CDS             complement(5619..8684)
                     /locus_tag="PLU99_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOO16514.1"
                     /translation="MPASLTPEQIELRTQRLGQEMFERIRGRQAWVLSKEWWLERLLN
                     LCMVNEWFKVQSFRFIDALPMLSDDVELARHLREYFVLPEHSRQRRENGQHGPSPTRE
                     WFVRGASRWMNWQRPDSFRARQTARFARAASIAMAGNFIAGSNVEEALRTIRTLRERK
                     LAFTIDVLGEAALSRAEAEAYHGTYMDLIDQLPGHAASWPAVPLIDEVPPVNVSVKLT
                     SLHPGFDAIAPEAAKRRVKELMRPLLRKAMSGGVFIHVDMEQYAIKDLTLELFEELFM
                     EDEFRDYPHVGIVLQAYLKDTDQDIPRAIEYAKKRGTPFWVRLVKGAYWDSETVWADQ
                     EHWPWPVWEQKWQSDACYERCTQLLVENHRHIHSAFASHNIRSLAHAIALRELYEAPA
                     TAFELQMLYGMGDPIKRAVVDMGQRCRIYTPYGPLLAGMAYFIRRLLENTANESFLRR
                     TADTPAAELLRDPQETGRRTPAFKKPTIVRYEFEEPLMNSFENVPNTDFSREPNRRQM
                     TAALEQARAALGRDIPLLINGERVTTGQWKESLNPSRPKEVIARVAQADAAAVDRAVK
                     AAAEAFTSWRRVPPDERAQALFRLAGVMEKRRFELAAWQALECGKPWREADADVSEAI
                     DYCNYYGKEMVRLAARPRSRAIPGEQNEYFYAPRGVVAVISPWNFPLAIPAGLIAASV
                     VTGNTVVFKPAGPAEVVGAKLAEMCGEAGIPGGVVNFVAGPGSAVGELLVKHPQVMTV
                     AFTGSREVGLRIYRLIAEAPAARPALKLFIPEMGGKNAIIIDSDADLDESIKAVMHGA
                     FGYAGQKCSACSRVIVLDAAHDRFMERLIESVRSAGVGPADNPATSIPPVIDRKAFDS
                     IRGYIEQGKKEARCVLEVDASGPTKEHGGYYIGPVIFDDVPPQSRIAQEEIFGPVLAV
                     IRAHSIEEAIEIFNGTDYALTGGIFSRSPGNIEKARAECECGNFYINRKVTGSRPDLQ
                     PFGGLKMSGFGARVGGPDYLVQFCDPRTVTENTLRRGFAPSKEVVETLG"
     gene            9283..10725
                     /locus_tag="PLU99_05335"
     CDS             9283..10725
                     /locus_tag="PLU99_05335"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOO16515.1"
                     /translation="MQLSPQDAQSSRVPGTELAAGRKAQMLRASSGLLLAGAALLLNV
                     GCSVPQPRGQGTLQRIVEPTRQATYWLYLPQDYVEANEAARQHRRWPVVVSFHGMKPF
                     DTAHAQAREWEQEADRYGFIVIAPELKAPDVLREFPIRRMSPSIESDERNTLAAVEHV
                     FSTTHADRGNVLATSWSSGGYLAHFMLNRHPERFTCLAVRQSNFSSQILDPYQVPRSR
                     DYPVLILTTQNDLGGVKQETKDAIQWYDQHAYPNLAWVEMKALGHERTPDMAAAFFAQ
                     VAGVQPNRPPTVLVNRQAIAGNPRGIAVLAGRMDSIRPGQPALATAVPPRSATPARSA
                     PGGDWSSSEAPALLAVRSMPRAEPATPSESSSGSAMMPSRAPERPALPRAPVTIRVSS
                     AIGTEPLVLAFSADCPADWQRSADFLWTLNGEPICSGVNGQKTITEPGEHTLGLLVVT
                     PGGEENRAYRLIRVLPSAESGRQAAAIGGN"
     gene            10805..11317
                     /locus_tag="PLU99_05340"
     CDS             10805..11317
                     /locus_tag="PLU99_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOO16516.1"
                     /translation="MAEFNRDVWAPWRMQYIESLSERDAGGCFLCRHVAAPQDDPQNH
                     VLWRSPKSLVVLNRYPYNTGHLLVAPTEHRAELEDLSDDVLCEMVWRIRDAKRVLQRT
                     LHPQGFNIGMNLGRCAGAGVPDHLHWHIVPRWGGDTNFMPVIADTKVIPEALDRTYEK
                     LVQAARELGL"
     gene            complement(11340..12089)
                     /locus_tag="PLU99_05345"
     CDS             complement(11340..12089)
                     /locus_tag="PLU99_05345"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOO16517.1"
                     /translation="MQHLMQLASVLSLAAPAQIQESMQAAFYPVLFGLLALASLGIPI
                     PEDVPLIAAGVLLHTHPGIASWPGTLLVALVGIMTGDLVLYRLGRRWGPGVVSHRSVS
                     WLLTPALFRKATQRFNRWGTWFCFFGRFFVGVRAAMCLTAGATGFPYWRFFLADFAGA
                     LLSVPLFIGLGYWFAEIVPKLRTYTEVVQVLITVGVVGVLGAIFLLRRRRRRKRVAEL
                     QSARAAGKRNQAAFSSRSGPGGASAAPCGGE"
     gene            12440..12880
                     /locus_tag="PLU99_05350"
     CDS             12440..12880
                     /locus_tag="PLU99_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16518.1"
                     /translation="MGRYAETRKTKNAFVRFRSLALLVGCASVTLAGCSRQEPSPPYQ
                     ALSGVVRAIDCETGELLIRAERPADRNLVCVVTKDSELYVNDRFSDLREVRIDDALDA
                     IAYRDRDRFVLSLVNIRRREPAPPAPAFLFKVTTQPTSSDKHED"
     gene            12882..14183
                     /gene="eno"
                     /locus_tag="PLU99_05355"
     CDS             12882..14183
                     /gene="eno"
                     /locus_tag="PLU99_05355"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOO16519.1"
                     /translation="MLFDEMRIEHVAAREVLDSRGNPTVAADVILSDGTFATAAVPSG
                     ASTGENEAVELRDGDKNRYLGRGVLKAVENVNRSIAQALIGMDPFDQELIDARMIKLD
                     GTPNKGKLGANAILAVSVAVAKAAAAAAGLPLYRYLGGASAHVLPVPMMNILNGGKHA
                     DNNVDFQEFMIQPWGAPTFREALRMGAEIFHSLKAVLKQRGYNTAVGDEGGFAPSLKS
                     NEEALEVIALAVDGAGYKLGKDVFIALDPAASEMWDEATGKYRFFKSQPDRLATSDEM
                     IDLWKCWCQKYPIRSLEDGLAEKDWDGWAKLTRTLGDKVQLVGDDLFVTNVHYLERGI
                     RERSANAILIKINQIGTLTETFAAVNLAMRNGFAAVISHRSGETEDTTIADFAVATNA
                     GQIKSGSASRTDRVAKYNRLLVIEEELGDAAVYGARFWRGA"
     gene            14192..14725
                     /locus_tag="PLU99_05360"
     CDS             14192..14725
                     /locus_tag="PLU99_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16520.1"
                     /translation="MSTQRHAGNFLFWFMLISGGLILAPAMILPAWVEYQAALDLRAR
                     HAEQVARWEAEITRLRKQREYLENDDSYALRLAYEDLNVNVPGIERIPVAPVEDADEP
                     PADDAEQLFIQPPEELAPELSALVETVLDRYPLAGMFLWQRTRPSLLFIGAGLVFTAI
                     VLVGEPVRKTDPQGVEA"
     gene            14959..>15556
                     /locus_tag="PLU99_05365"
     CDS             14959..>15556
                     /locus_tag="PLU99_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16521.1"
                     /translation="MNAAFRWEQPDYLVLVLAAACALAAYGWSGRAHRSRARQSNRTL
                     MHQLARLTVALIAGSAAALVGTALINLRTPNSALALTVAAALSAAWLAARLYRRRNES
                     TAGGAFRLLIALRVLAALTILAIVARPVWEWVEVTWRKPLLAVVLDQSQSMSIRDDQS
                     QRGRSRADLANAALQANRAAIEKLKSLYEIRVYSPGSAA"
BASE COUNT         2876 a         5319 c         4650 g         2711 t
ORIGIN      
        1 ggccggcggc ctccagccgg cgcagcagct cgatgccgtt gccgtcgggc aggcccaaat
       61 cgctgataag caggtcaaat ttccgtttgc cagcctcgtg cagtgccgcc gacacggtcc
      121 cgacggccgt tacgcaatga ccggcagctc tcaaaagctg cgaaatgatg cgcgcggtat
      181 cgccgtgatc ctccaccagc agaatttgca ggccgcgctt ctctttcgcg gagggacgcg
      241 cgggtcgagc ggcctgggct ccgcccacgg tgtcagcccc gaccggcaat gtaacgctga
      301 acgtcgcgcc ccggcccctt cccgggctgc cggccgcgat cgtcccgccg tgcatttcca
      361 ccaacgcctt gctgatcgcc aggcccaagc ccagtccgcc gaactggcgt gcagagtccc
      421 gttcggcctg ctcgaaggca gtgaacaggc gcggcagcac gtccggttca atccctatgc
      481 cgctgtcgct gacctcggtt atgagccggc cgtccgcacg gcggcagcgg atacctatgc
      541 gaccgccggt gggcgtgaat ttgatggcgt tactcagcaa gttccagaat acctgctgca
      601 gacgcgtcgc gtcggcgtca atcgtgcaga tgccggcctc gcccaggtcc atacagagtt
      661 ccagccgccg gctctggatt tcgggcatgc acacctccac tgcctggcgg ataacgtcac
      721 ccaactgcac gggatggcga tgcatttcca gcttgccacg ggcaatgcgc gtgacgtcca
      781 gcaggtcatc gattagccgg gcctccagct tgacgttgcg caggacgagc tcgagcttgt
      841 cgcgcagctc cgcgccgagc cggtcgtccc ttaccaacat ggataccgca cccaggaccg
      901 gggtcagcgg cgtgcgcagc tcgtggctga gcaccgccag aaactgatcc ttggcgtggc
      961 tggccgcctc ggcgatggcc atggcccgct ccgcgccttg cttggcggca gccaacgcct
     1021 tctcggcgcg cctttgttcg gtgatatcgc ggacggccgc gaaacgcact acccggttct
     1081 ggtacgtaac gggtgtgacg cggacctccg cggtaactcg gctcccgctc ttccgcagca
     1141 gcgtgtgctc aatctgcgcc tggcgggcag caacgaccgt tcgccgaacg agctcacgat
     1201 cttccggcgg aactagagcc tccacttcca tcccaatcag ctcatcgcgg ctgtagccga
     1261 gcatgttcag gaactgctcg ttggcgtcga cgagccgccc gccttcctcg atggcaatgc
     1321 cttcaaaggt ggcagccgcc aaagctttga atcgttcttc actctcgcgc agggtcagct
     1381 cggcccgctt gcgatcggta atgtcttcgt aggtgcccag cacgccaaag accttgccgt
     1441 ccggacctcg cagaggaatt ttgctggtcc gcaaccacag caactggccg tctggcgcgg
     1501 tctgcggctc ttcatacccc agcttggcct gccccgactc catgacggct tggtcgtctg
     1561 cacggtatag acgcgcctgg tcgcgccagc ccatggcgta gtcatcccgg ccaatgatct
     1621 ggtccggcga ctccagccct gcgtcgcgcg cgaagtgctg gttacagcca agaaaaacac
     1681 cgttcacgtc tttccagaat acgcgtaccg gaaccgtgtc cagcaccagg cgcaacatct
     1741 cctgcgactg acgcagtgcc tgctcggcgc gcttgtgccg cgtaatatcg ctgctcgctc
     1801 ccaccgcgcg cacaggcttg cccgactcat ctctgatcgc caggtaatcg tcctgaatcc
     1861 acctttcctc gccgtccggc cgcacgatgc gatactcggc cccccagtgg tcttcgtttg
     1921 actcaagcca cttcaagaat tgcggataga ggttagcggc gtcctcgggg tgcatgaatt
     1981 cccgcacgaa gcgatcgatc gtcagtcgtt ccccgggctc gatgcccagt atgcgcgccg
     2041 tcaactcgtc caggcatgcc acgcccgtcg ccaggtcaat ctcctgcacg ccgatctgcc
     2101 cggattgagt cgccagacgc aggcgctgct cgctggccgc tagggcctct ccggcttcct
     2161 tgcgcgccgt gacgtcctcg tacgtgccca gcacgccgat cactttcccg tccggagcgc
     2221 gcaaaggcac cttgctggtc cgcagccacc gccggcaccc gtcaggcccc gtgaccggac
     2281 gttcgtaatt cagaatggcc tggccagtct gcattaccaa ctcgtcatcc acccggcata
     2341 tctcaggctg ccagcgccgc tgcaggtcgc aatccctctt accgatgacc tgctcggagg
     2401 aatcaaagcc cgcgtcgcgc gcgaaatgaa gattacaacc caaatagatg cagttcaggt
     2461 ccttccagaa gacgcgcacc gggaccgcgt ccagaacctc gcgcagcatg tgctgcgagg
     2521 cgcgcacttc ctccgccgct cgggccaaat ccgcctgcac gctcagttcc ctctccgccc
     2581 gcaccgcccg gtcgcgcgcg cggcggtaca gcccggcgac gacgctgacg aacacgccca
     2641 tgccgaaaaa tagagccagg ttgacgtctg cgtgcagcgt gcgatcatgc tcaagcggca
     2701 gtatcagcca accggccagc agggccgcaa gcccggtagc cagcaatccc ggtcccaacc
     2761 cgcccagcaa agctgcgatc atcagggcag gataaaagaa gatgtatggc ggcagttcgc
     2821 ccgcgaaccg ccccatcacg agatgcgcgg cacacgccag cagcgtcgcg cctaccgcaa
     2881 ggccgtaatc agtgaggctc ctgcggagcc gaacattttg catcgtccgc tcccgaacct
     2941 ctcggcaaaa caaaatgacg ctgtctctta aaatagtgta ggagggttat gttggaattc
     3001 agcgccccct ttttccacag ctcacagact cttcagaaat ctgcccgccg accaggctca
     3061 agccgcaccg gacggccgag aatctgagca tcaacgggcc ggtcaccgcg gtgctcgacg
     3121 cggatccggc cctgctcgat ggttataaag agtggttgac cgcgccacac gaacgggaag
     3181 cgcagagcgg tccagttccg cggcaggtgc ggatcgatcc acaacacttg tgattgcatc
     3241 agcgtgcgaa ggcccgcaaa accgaaaacg acgacctgcc agttgagccc cgccgccgcg
     3301 gccgcgacgc cttccgctgc gcccggtgga tcgaggttcc gggatgcaga ttcgccccac
     3361 aaacgcaggg cctcgtccgt gcgacccagc cacgcagcca cgagcgcaaa catgccccgg
     3421 ctgacgctgg aatcatgcgc tgtcagcggc gcgtaagtat cgaaggcggc ctgcatctgc
     3481 tcgacggcga attcttgcgg gaacaccgca atgagctgga gcgcgtccgc ttgcttgatc
     3541 acctggctgc ggaaaaggcg ttcctggggc acactggcgg ccagcggacg cgcgcggtcg
     3601 gtccagtgcc ggtccagctc aaagggctcc tgctcgaaaa aaccatcgca ttgcacgacc
     3661 agcttgcgag cttcatcgta cgggaaccgc agtttgccgg ccgtctccgt gaactgctcc
     3721 agttccgaag cttgcagtgc cagccgcgcg ctcagctcaa ccgcggccgc ccgatcgcgt
     3781 gactccagct ctcgccatgc ggtcaccgcc aggttcaaag cgcacgccac gagccggttt
     3841 gtgtaggcgt tattgcggct gaagggcgtg tactggtccg ggccggtgac catcaacagt
     3901 tcatactctc cgcgagccgc gttgtaagtg acgcgctgta gccagaagcg cgcaatttcg
     3961 gtcagcgcct cggccacgcc ggccagaaac ttcacgtcgc tggtgttggc caggtaggcg
     4021 tgccagagtc cgtaggccac atccgcactg acatgcacgt cgtggtcagc gtgctgccag
     4081 gccgggcatt gctcctgccc ctgcgggtcg ctttcccacg catagcgcgc gccggagtaa
     4141 ccgtaacgcc gcgcgttcac gcgtgccccc gggagcgacc tgagacgaaa accggccagc
     4201 gtccggccga cctccgggcg gctgtacagg aaagcgggca gcatgaagac gtcggtatcc
     4261 caggtgaagc gcccgcaaca ggcctcattc gtggccgcgc agggatcgat cgccagtcgc
     4321 gtgtccccat cgccggccgc tcgcatgagg tgatacagcc cgcagcgcaa cgcgagctga
     4381 ctgcgcggat cgccttcgat gacgacatcg gtctgctccc atctgcgctg ccagaccgcg
     4441 tccgattccg ccgccagccg ctcgtagccc aggcccgccg cggaccaaac caagttacgc
     4501 gcggcgtcca gcgggcggcc ctgcacgtgc cgggaggaga tcaacaccgc gtacttgcag
     4561 agcgttacct gctggccggg ttcgagcagg cgcatgccgc cgatgcccgc ccagcgcggc
     4621 tcggtttcaa ccgtccacac tacgcccggt tcgcaggtca tcagggcggc ggccgcaacg
     4681 gtgtcgccgc cgttggtgcg cacctcaatt gtgattggct cgtgctcgcc ggtaagcgcg
     4741 atgtgctcga agtggtcgaa accgttcgtg cgaacgtcgg cgtccagcgt gcccaccatg
     4801 cgcagctcga ccgcgggccc gctgaggtgg cgcaaatgac agcgcaacgc catcacatgc
     4861 cggcgagcgg cgctgatgaa acgctcgaac cgcacggcga cggcgccgcc acgctgcgtg
     4921 tgccaggtga actcgcggct aagtcgcccg gtgcgcaggt cgagccggcg gcggtagccc
     4981 tcgatgcggc tgcgctccat gtcgaggcgt tcgtggccgg catagagggt taggccgtga
     5041 atcgccggca ggttgatcat ttcgtcgccg caagtcgggt gcggcccggt tacgccgggc
     5101 aggaacgtac cgaaccggct cttggaactc gtggagttgt ggggccggcg ccccgccggt
     5161 ggtcccgcgc cttgagggag gtgcaggtac tcacgatcct gcgggtcatc gctcaaaccg
     5221 ccctcaagcg aggcacgctg ctgtagcggg ccgctgccga tagccatgag ggcctcatag
     5281 tggcggttgt tgcgtacatc aaaatcggtt tcgacgatgg cccaggtatc gacagtcgaa
     5341 gtgtccagag gttcggtcaa gcgttctctc cgagcagagc cggctcatgc cgagccggaa
     5401 ttcccccatt atgcctcatc ctgcgcgggg agtcgaagaa cggaagcggg cgtggccagg
     5461 tgaggacgac aggaacccgg cgcaagcagc ccatcgaacc gacgcgcaca tcattctgcg
     5521 tcaacagcag ccgcctcgcc ggcgcttgac tgtgccgagg caccagccct tatcgcggac
     5581 cctcagctcc ctcccttgct gcacccccct gatccgcgct atcccagcgt ttccacaacc
     5641 tccttgctcg gcgcaaaacc gcgacgcagc gtgttttcag tcaccgtgcg cggatcgcag
     5701 aactgcacca ggtaatccgg accgcccacg cgtgccccaa agccggacat cttaaggccg
     5761 ccgaacggct gcaaatccgg gcgcgagccg gttaccttgc gattgatgta gaaattgccg
     5821 cactcgcatt cggcgcgggc cttctcaata ttgcccgggc tgcgggagaa aatgccgccg
     5881 gtcagggcgt agtcggttcc attgaaaatt tcaatggcct cttcgatcga gtgcgcgcga
     5941 atcaccgcca gcaccggccc gaagatttcc tcctgggcga tacgggactg cggcggaaca
     6001 tcgtcgaaaa tcaccggccc gatgtaatag cccccgtgtt ccttcgtcgg cccgctcgca
     6061 tccacttcga gtacacagcg ggcttccttc ttaccttgct ctatgtagcc gcggattgaa
     6121 tcgaatgcct tgcgatcgat caccggtgga atcgacgtgg ccgggttatc cgccggaccg
     6181 acgccggccg agcgcacgct ttcgatcaag cgttccatga accgatcatg cgctgcatcc
     6241 agcacgatca cccgcgaaca ggccgagcac ttctggcccg cgtagccaaa ggcgccgtgc
     6301 atgaccgcct tgatggattc atccaggtcg gcgtcggaat caataatgat cgcgttcttg
     6361 ccgcccattt ccggaatgaa gagcttcaac gccggccgcg ccgcaggcgc ttccgcaatc
     6421 agccggtata tgcgcagccc cacctcgcgc gagccggtga atgcgaccgt catcacttgc
     6481 ggatgcttca ccagcagttc accaaccgcg ctgcccggac cggccacgaa gttgaccacc
     6541 ccgcccggta taccggcctc gccgcacatt tccgccagct tcgcgccgac cacttcggcc
     6601 ggaccggccg gcttgaatac caccgtatta cccgtcacga ccgaggccgc gatcagcccg
     6661 gccgggatgg ccagcggaaa attccacgga ctaatcaccg ccaccacgcc gcgcggcgcg
     6721 tagaaatact cgttctgctc gccgggaata gcgcggctgc gcggacgggc cgccaggcgc
     6781 accatttcct tgccatagta attgcagtaa tcaatggctt ccgagacatc ggcgtcggcc
     6841 tcgcgccagg gcttgccgca ttcgagcgcc tgccacgccg ccagttcaaa gcggcgcttt
     6901 tccatcacgc cggccaaccg gaaaagcgcc tgtgcccgct cgtcaggcgg cacgcggcgc
     6961 cagctcgtaa acgcctccgc cgccgctttc accgcgcgat ccaccgccgc cgcgtcggcc
     7021 tgggccacgc gcgcgatcac ttccttcggc cgcgacgggt tgagcgattc cttccactgt
     7081 ccggtcgtaa cgcgctcgcc gtttatcaag agcggaatgt cgcgccccag cgcagcgcga
     7141 gcctgctcca aggccgcagt catctgccgc cggttcggct cgcgcgaaaa gtcggtattt
     7201 ggtacgttct caaaggaatt cattagtggt tcctcgaact cgtagcggac gatcgtgggc
     7261 ttcttaaatg ccggcgtgcg gcggccggtt tcctgcgggt cgcgcagcaa ctcggccgcg
     7321 ggcgtgtcgg ccgtgcggcg caagaacgat tcgttggccg tattctccag cagccggcgg
     7381 atgaaatacg ccatgccggc cagcagcggg ccgtacgggg tgtagatgcg gcagcgttgc
     7441 cccatgtcaa ccaccgcccg cttgatgggg tcacccatgc cgtacagcat ctgcagctcg
     7501 aacgccgtag ccggcgcctc gtacagttcg cgcaacgcga tggcatgcgc caggctgcga
     7561 atattatggc tggcgaacgc gctatgaatg tggcggtggt tctcgaccag gagctgcgtg
     7621 cagcgctcgt agcatgcgtc ggactgccat ttctgctccc acacgggcca aggccagtgc
     7681 tcctggtccg cccacacggt ctccgaatcc cagtacgcgc ctttgaccag ccgaacccag
     7741 aagggtgtgc cgcgtttctt ggcgtattca atagcccgcg gaatatcctg gtcggtgtcc
     7801 ttcagatacg cttgcagcac tatgccaaca tgcggatagt cgcggaactc gtcctccatg
     7861 aacagctctt cgaacagctc cagcgtcaag tcctttatgg catactgctc catgtcaaca
     7921 tggatgaaga ctcccccgga catcgctttc cgcagcagcg gccgcatcag ctccttaacc
     7981 cgccgtttcg ccgcctcggg cgcgatcgca tcgaacccag ggtgcagcga ggtcagcttc
     8041 accgacacgt tgacgggcgg tacctcgtcg atcagcggca cggccggcca gcttgcggca
     8101 tgaccgggca actgatcgat caggtccatg tacgtgccgt gataagcctc cgcctcggct
     8161 cgtgataacg ccgcttcgcc cagcacgtcg atcgtgaacg caagcttgcg ttcccgcagc
     8221 gtgcggatgg tgcgcagcgc ctcctccacg ttcgagccgg ctatgaagtt gcccgccatg
     8281 gcgatcgagg cggcgcgggc aaaccgggcc gtctgccggg cacgaaaaga gtccggccgc
     8341 tgccagttca tccagcgact ggccccgcgg acgaaccact cgcgcgtcgg cgaggggcca
     8401 tgttgcccgt tttcgcggcg ctgccggctg tgctcgggca ggacgaagta ctcacgcaaa
     8461 tggcgggcta gctcaacgtc atcgctgagc attggcagag cgtcgatgaa ccggaaagac
     8521 tgcaccttga accactcgtt aaccatgcac aggttcagaa ggcgctcgag ccaccactcc
     8581 ttgctgagca cccaggcctg ccggccgcgg atgcgttcga acatctcctg gccgagccgt
     8641 tgggttcgca gttcaatctg ttcaggagtc agagaggcgg gcatcttttc aacccttggt
     8701 gagcgttccg ggcgggccta aaccccgccc cgcattagcc atgcaaccac cgtgccgtgc
     8761 caactgcttc atgccggccc gcgccgcgcg gtcaaaagcc gatggcaacc acggcgatgc
     8821 aactgcacgt cgaattccac atttcgctgc aaaaaccatg ctaacaagcg ccatgacaac
     8881 gggcaaacag gtccgctggt gcgcgtcggc acgctacgtt ccggtgcagt taccgcaacg
     8941 ggcgggggcg atcgaaacgt tgaccggcgc tcgtgccgcc cggaagtgcc gttcgcgcaa
     9001 tggggtcttc atccgatgtg ggcattccgc ttaaccggac ccgcaacgtc gccggcggta
     9061 ggcgatcgcc acatattcga cccacttagc tgcgtgcaag cacacggttt tcggacctaa
     9121 ccccgggttc aagcaatcta tcgttgggga tttcccccta cctcgggctt agttacaatg
     9181 tctttatgtc cgtgtgggtt atcgcgcggt catggatcga ggatgcctgc cccaaacgga
     9241 tagccgatat agagccgcgg cacgatagac aagggccaga ttatgcaact atctccgcag
     9301 gacgcgcaat cgagccgcgt tccagggacg gaactcgccg cgggccgcaa ggcgcagatg
     9361 ctgcgcgctt cgtccggcct cttactggcc ggcgccgcct tgttgctgaa cgtaggctgc
     9421 tcggtcccgc agccgcgcgg ccaaggcacg ctgcaacgca tcgtcgagcc gacccgccaa
     9481 gccacctact ggctgtactt gccgcaggac tatgttgagg ccaacgaggc ggcccggcag
     9541 catcggcgct ggccggtagt ggtttcgttc cacggcatga agcccttcga caccgctcat
     9601 gcccaggcgc gggagtggga gcaggaagcg gatcgctacg gcttcatcgt gatcgccccg
     9661 gaacttaagg ccccggacgt cttgcgagaa ttcccgatcc gcaggatgag ccccagcatt
     9721 gagtcggatg aacgcaacac gctggcggcg gttgagcacg tttttagcac cacgcatgcc
     9781 gatcgcggca acgtgctggc cacaagttgg tcgtcgggcg gctacctggc gcacttcatg
     9841 ctgaaccgtc atccggagcg ttttacctgc ctggcagtgc ggcagtcgaa tttctcgtcg
     9901 cagattctcg acccctacca ggtgccgcgc tcgcgagatt acccggtcct gatccttaca
     9961 acccaaaatg acctcggagg cgtgaagcag gaaaccaagg acgccatcca atggtacgac
    10021 cagcatgcct acccgaacct ggcctgggtc gaaatgaaag ccttaggtca cgaacgcacg
    10081 cccgacatgg cggcggcttt cttcgcgcaa gttgcgggcg tccagcctaa tcgcccgccg
    10141 accgtcctgg tcaaccgcca ggccatcgcg ggaaatccgc gcgggattgc cgtccttgcc
    10201 ggacgcatgg actcgatcag gcccggccag ccggccctgg caaccgctgt tccgccgcgc
    10261 tccgccaccc ccgcacgcag cgctccgggc ggggattggt ccagctccga agcccccgcc
    10321 ctgctggccg tccggtcgat gccgcgagcc gagcccgcca cgccatccga atcgagcagc
    10381 ggttccgcca tgatgccctc gcgcgctccg gaaaggcccg ccttgccgcg ggccccggtt
    10441 acgattcgtg tatcctcggc catcggcacc gagccgttgg ttctggcctt ctccgccgat
    10501 tgcccggccg actggcagcg tagtgccgat ttcctctgga cccttaacgg cgagccgatc
    10561 tgcagcggag tgaacgggca gaaaacaatc accgagccgg gggaacatac gctggggttg
    10621 ctggtggtta cgcccggcgg agaggaaaac cgcgcgtatc gtctgatccg ggtactgcca
    10681 agcgcagaat cggggcggca agcggccgcg atcggcggca actgaaccgc cttccgcccg
    10741 gccggatgat gcataccggc cgccccggcg ccgcggttcc ccggtgcttg aaggaacgcc
    10801 tcacatggcc gaattcaatc gtgacgtttg ggcgccctgg cggatgcagt acattgaatc
    10861 gctcagcgag cgtgatgccg gcggctgctt tctttgccgc cacgtcgcgg cgccccagga
    10921 tgacccccaa aaccacgttc tgtggcgcag ccccaagtcg ctggtcgtgc tcaatcgcta
    10981 cccgtacaac accggccacc tcctggtggc tcccactgag caccgcgcag agctcgagga
    11041 cctctcagat gacgtgctct gcgaaatggt ctggcgaatc cgcgacgcaa aacgggtcct
    11101 gcaacgtact ctgcacccgc aggggttcaa catcggcatg aatctgggac gctgcgcggg
    11161 agccggcgtt ccggatcacc tgcactggca catcgtgccg cgctggggag gcgatacgaa
    11221 tttcatgccg gtcatcgcgg acacgaaagt gattcccgaa gcgctcgacc gtacgtacga
    11281 aaagctggtt caagccgcgc gcgaactagg cctttagaac gcgtcgaatt tgcccgcgct
    11341 cattctcccc cgcagggcgc cgcactagcg ccgccgggtc cgctgcggct cgaaaacgcc
    11401 gcttgattgc gcttgcccgc cgcgcgcgcg ctctgaagct cagcgacccg tttgcggcgc
    11461 ctccgcctgc gcagcaaaaa gatcgcgccg aggacgccca ctacgcccac cgtgatcagc
    11521 acctgcacga cctcggtgta cgtccgcagc ttgggcacaa tctccgcaaa ccagtaaccc
    11581 agcccaatga acagcggcac ggatagcaat gcccctgcga aatccgccag gaaaaaacgc
    11641 cagtacggaa acccggtcgc cccggccgtc aggcacatcg ccgcccgcac ccccacaaag
    11701 aaccgcccaa agaaacagaa ccacgtcccc caacggttga atcgttgcgt agccttgcgg
    11761 aacaacgccg gcgtcagaag ccagctcacc gagcgatggc tcaccacgcc cggtccccag
    11821 cgccgcccca gccggtaaag aaccaggtca cccgtcatga ttccaaccag ggcgaccaac
    11881 agcgtccccg gccagcttgc aatgcctggg tgcgtgtgta gcagtacccc cgcggcgatc
    11941 agcggaacat cctcgggtat cgggattccg agactggcca gcgccagcag gccgaatagt
    12001 accgggtaaa aagccgcctg catcgactcc tggatttgag caggtgcggc caacgatagc
    12061 acggaagcca gttgcatcag gtgttgcact agaagctcgg tcgttggatg cagccgggct
    12121 cgacgctcgc cggccggggg cctacaggca agttctcttc tgcctcaagg tgcaacagtt
    12181 tagccaaatc tccgctcgtg accagcgtaa tctgtaacgt ctgtaatctc cgttaagcgc
    12241 gctccacctt ttgcccgctc agcgcgcgtc cctcccaaat cccgaagctc tcctgcgggt
    12301 tggctgagcc ggcccgtgtc gagcatgaat ccacgtttct aaaacgaacg cgggcggact
    12361 tccttttgcc gggcggcccg cacgatacaa tgttacgcaa atattgtcgc cgaggtactc
    12421 gcctgattag gtccgctgaa tgggacgtta cgcggagacg agaaaaacga aaaacgcatt
    12481 cgtcagattc cgctcgctgg ccctgctggt cggctgcgcc tccgtcaccc tggccggctg
    12541 ttcgcgccag gaaccctcgc cgccctatca ggcgctcagc ggcgtggtgc gggcgatcga
    12601 ttgcgaaacc ggcgagttgc tcatccgcgc cgaacgcccg gcggaccgta atctggtgtg
    12661 cgtcgtcacg aaggactcag aactctatgt caacgaccgg ttcagcgatt tgcgcgaagt
    12721 acggatcgac gacgcgctgg acgccatcgc gtaccgcgac cgggatcgct tcgtgctgag
    12781 cctggtcaat atccgccgtc gtgaacctgc gcctcccgcg ccggcgttcc tatttaaggt
    12841 gacaacccaa cccacttcct ccgacaagca cgaggattag catgctattt gatgaaatgc
    12901 gcattgagca tgtggccgcg cgtgaagttt tggactcacg cggaaatccc accgtcgccg
    12961 ccgacgtaat actttccgac ggcaccttcg ctacagccgc cgttcccagc ggggcctcca
    13021 ccggcgagaa cgaggcggtc gaactgcgcg acggcgacaa aaaccgttac ctcggccgcg
    13081 gcgtcctgaa ggccgtggaa aatgtcaatc gctcgatcgc ccaggcgctg atcggaatgg
    13141 atcctttcga ccaggaactg atcgacgccc gcatgatcaa gctcgacggc acgcccaata
    13201 aaggcaaact cggggccaac gccatcctgg ccgtctcggt cgccgtcgcc aaggccgccg
    13261 cggccgccgc cggcctgccg ctctatcgct atctcggcgg cgccagcgcc cacgtgttgc
    13321 ccgtacccat gatgaacatc ctcaacggcg gaaagcacgc ggacaacaac gtcgatttcc
    13381 aggaattcat gatccagccc tggggagcgc ccaccttccg tgaggccctg cgcatggggg
    13441 ccgaaatatt ccattctctc aaagccgtgc tcaaacagcg cggctacaac acggccgtcg
    13501 gagatgaagg cggctttgcc ccgagcctca agagcaatga agaggcgttg gaggtcatcg
    13561 cgctggccgt cgacggcgcc ggttacaagc tgggcaaaga cgtattcatc gcgctcgacc
    13621 cagccgcctc ggagatgtgg gacgaggcca ccggaaagta ccgcttcttc aaatcgcagc
    13681 ccgaccgcct cgccaccagt gacgagatga tcgacctctg gaaatgctgg tgccagaaat
    13741 accccatccg cagtctggaa gacggtctgg ccgagaagga ttgggacggc tgggccaagc
    13801 tcacccgcac gctgggcgac aaggtgcagc tcgtgggcga cgacctcttc gtaaccaacg
    13861 tccactatct cgagcgtggc atccgtgagc gcagcgccaa cgccatcctg atcaaaatca
    13921 accagatcgg gaccctgacc gaaaccttcg ccgccgttaa cctggccatg cgcaacggct
    13981 tcgccgccgt gatcagtcac cgcagcggcg aaaccgagga tacgaccatc gcggatttcg
    14041 cggtggccac taatgccggc cagatcaaga gcggcagcgc ctcacgcacc gaccgcgtcg
    14101 ccaaatacaa ccgcctgctg gtcatcgaag aagaactggg cgatgccgcc gtctatggag
    14161 cgcgcttctg gcgcggcgcc tgacaccggc catgagcacc caacgccacg ctggaaactt
    14221 cctgttctgg ttcatgctga tctcgggcgg cctgattctg gcgcccgcca tgattctgcc
    14281 ggcctgggtg gaataccagg ccgcgctgga cctgcgcgcc cggcacgccg aacaggtagc
    14341 ccgctgggaa gctgaaatca cccggctgcg aaaacagcgt gaatacctcg agaacgacga
    14401 ttcgtacgcg ctgcgattgg cttacgaaga cttgaacgtc aacgttcccg ggattgaacg
    14461 catccccgtc gcgcccgtcg aggacgccga tgaacctccg gccgatgatg cggaacagtt
    14521 gttcatccaa ccgcccgagg agttggcgcc ggagctgtcg gcgttggtgg aaaccgtgct
    14581 cgaccgctat ccgctggccg gcatgttcct ctggcagcga acgcgcccgt cgctgctgtt
    14641 catcggcgcg gggctggtct tcacagccat agttctggtc ggtgagcccg tgcgcaaaac
    14701 ggacccgcaa ggcgttgaag cgtagcagag ccggggcgta tcgcctaggc gagcagagcc
    14761 ggggcgtgcc gccccggaat ccgaccactg gcgccccgac ctcgccgcca acatgatgaa
    14821 ccccgatttg gccgcaaacg ggataatggt cgcgcgcccg gaacccgacc attgttgccc
    14881 cgatttcgcc gccaacgtga taatgcatcg tacgctcgtt gtatccgtag aggctgccgt
    14941 gggcgacgga gtagctgatt gaacgccgcc tttcgctggg aacaaccgga ctacctcgtc
    15001 ttggtactcg cggcggcgtg tgcgcttgcg gcctacggct ggagcgggcg cgcccaccgg
    15061 tcgcgcgcgc gccaatccaa tcgcacgctg atgcaccagc tagcacgtct gaccgtcgct
    15121 ctgatagccg gctcggcggc agcccttgtc ggcacggccc taatcaacct gcgaacaccg
    15181 aatagtgccc tcgccctcac cgtggcagcc gccttatccg cggcatggct tgccgctcgc
    15241 ctctatcgcc ggcggaacga atcaactgcc ggcggtgcat tccgcctgct gatcgctctc
    15301 cgcgtcctgg ccgcgctgac catcttggcc atcgtggcgc gcccggtctg ggagtgggtg
    15361 gaagtaacct ggcgcaagcc gctgctggca gtcgtgctcg accagtccca atcgatgagc
    15421 attcgcgatg atcaatcgca acggggccgt tcacgagctg acttggccaa cgcggccttg
    15481 caggccaatc gcgccgcgat cgaaaagctg aagagcctgt acgaaattcg cgtctactcg
    15541 ccgggttccg cagccg
//
LOCUS       DAOSYS010000059        15278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3257_length_15278_cov_5.525586, whole genome shotgun sequence.
ACCESSION   DAOSYS010000059 DAOSYS010000000
VERSION     DAOSYS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15278
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3257_length_15278_cov_5.525586"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="PLU99_05370"
     CDS             <1..1495
                     /locus_tag="PLU99_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO16522.1"
                     /translation="ADQRFWAEQMISAGQSDAPQSAASQPGARASDAMAGRVRSIERA
                     MAELAAAVAGPLDRELTGRYAWKLRNAELILDASRPQAGPTVLVRHAEDQIIGVHNVV
                     LGGQCKEVLELSECDSYDPMQSRIAAGWWAGGEDLDEENTRTFWRCATNGHMAAVAVQ
                     DGLFGLGLGCARGADKLWHRTLAEWGGIAAHPGEALAGGTAGVYVGWRQDRVALLDWA
                     DGSPRWERSLPGLRIDQLLSLGERLIIVGRDGDVVSVDANCGDDLRSLSPVGGAPRAV
                     TTAGETLVVWTEDRVGGYDPATFVCRWLRPSRGVEDYKCVAGSNWVLYRDQGETQWGL
                     IDADTGDPVFAGRWGAFGGTLTAAAKDGERLLIAAFSRKAASGDAAPLVHLAAFDAES
                     GAQLWERDVPTLARVNQTQLQASADLIPVLVARSDYEYTETRNYNALALCVVDKESGA
                     VTEPVSIMDDFRGTQGRCAAYLLATPSRIIVQANGTIAAYGPVSGRQ"
     gene            1495..2637
                     /locus_tag="PLU99_05375"
     CDS             1495..2637
                     /locus_tag="PLU99_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOO16523.1"
                     /translation="MIGACRDIGPSIMAALALLVCCYATTYSQVTPAGSAASDTSPAE
                     PPADPGSALNLPPQLPPGITPAAADSIQRGLRALVSLQNRDGSWRTGGSSGGYPTAMT
                     ALAGLALLAGGNTPTQGAYAENVSRGLSYLLSVARRDGLIASVEEESHCMHGHGFAML
                     FLSQCYGMEEDAQRRAQVGLALQRAVQLTARSQSAAGGWLYTPDSNGDEGSVTVTQVQ
                     ALRACRNVGIAVPKRVIDQAMQYLERSLNEDGGIRYQVSDRGPSRPAITAAAVACWYN
                     AGLYDSPHVAAALRFLKPRLTPDPNNDVHHFGHYFYGHLYLAQVLYQTSQRDWEEYFP
                     AMRDALIRAQERDGTWEGDAVGPVYGTSVALIILQLPYKNLPIMQR"
     gene            complement(2722..3813)
                     /locus_tag="PLU99_05380"
     CDS             complement(2722..3813)
                     /locus_tag="PLU99_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16524.1"
                     /translation="MRGRRLRLFASALLGLVISVLILWPTVAAGQPAATQTATTQPAR
                     PQIDRLNRAFLEHVGTLGPEYSRAAEAVREAWQSYQQSMPQSFVPDALGLLYPDYRDA
                     LNAFDEQRYGDMQRLAGPLRESRDPFLAANAQYLYVRALIEQGLLEEAEHELRNAAGP
                     AAGGPELEQYTPYAGHLRFMRAYCEASNLRFAEALQTLQSLEADIPDAPEPVQAGARQ
                     LRLEIERRERGTLDEVAMLMSYSAARLTVADATQRVRSRQDEAVALLDKLVEEAQERE
                     KSQDGAGQRRAAQDKRPGAPRERSEAPESGAAPIGELHRAPAADPSQMWGQLPPAQRE
                     RILDSLRDRFPSRYRQLVEQYYRSLAEER"
     gene            complement(3815..5596)
                     /locus_tag="PLU99_05385"
     CDS             complement(3815..5596)
                     /locus_tag="PLU99_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16525.1"
                     /translation="MRAEAGNSGFCVRPARCAALLIVGCALLVHVAPRSALAQPAGTQ
                     PTLLQRQQDIRQRLQELESRMNLLARVLAESEPDKADRLRTATDLCGQKQIKRRLDDI
                     VFLVKSGDYSAAERAQGELLADLEEILQQLQDTGSDFDRKRAERERLERLKRGIQSLM
                     QQQLEQLYRTQAAAPDGELARRLREQADALEGLAERQAELRKRATGAEQQELADSAEQ
                     HAAELDKLRQQTNQAEVAVALEQSAGETQDSAAKMRAASEKLEAQEDAEAGLDQQAAE
                     EQLRRSARRLREQAQRLAERAKVHELEQQQRALAQGAGGLEKELSPKPGKAGPGTPGQ
                     RQVGNAQRSMQKAAERLAEQRVRAAEPEEEAALEQLQRALDELDDALRQVRREELEET
                     LTALDARFKSMLARQRQIRDDVSALHEKAPRGWQRPDQLKLSETAQTQRAVAADCAEV
                     ARILVSEGTTVLLPELTGDLARNMEDIADRLDQSDTGVQTQRRIAEVLAALEEVVGAI
                     EVRRKQELQNMLQPPDQGGQSGRQALLPESVELMLLRSAQMRINQRTADLSADSAASP
                     EAAAELARLAARQRRLVEIARQMNERK"
     gene            complement(5608..7764)
                     /locus_tag="PLU99_05390"
     CDS             complement(5608..7764)
                     /locus_tag="PLU99_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16526.1"
                     /translation="MSVAPVEELASELRESVLAPLARLRRRACVYLALAGAQQLAVAV
                     AAASLVQFFLDWSLKLPLSQRIVLSGVLLLYWAVVVWRKLIVPLSRPMPDHLLAGLVD
                     RANPELHDRLSSAVELAEQMPSGRRPELVNTVLAEACRASADVDFSRILNHRRAARQA
                     AGTLGLILVIALAVCEFPSLAGPWLARNWLMQNVAWPQHTHLWPEGFDADGRRRMARG
                     DVIEISAAITGRVPDSAVLHWWTPSGRRGREEMSLVADTRLTASIGQLTEQLQFRITG
                     GDERTRVFTIEAVERPRVMHTRARITPPEYTRLEPLEIEQQTVLEILAGSLLEIEALL
                     NKPVQQARFVAADESDAPCGVSWGDPADAQAVVRLRWAAPQAGIYRFALLDFDNLTSR
                     NPVSYTLKVAPDAPPTVKLDLSGVGEMVTPQAELPVRLTCEDTYGLSAARLLVQTADG
                     PERTLSAPGFEPAARKYTGEFVLMMAAGGAAAGQTVRIRGEARDIRPGEPNAGTTPAT
                     SLRVVSPEDLLIEVARRELGLRQEFERLISAQAALADGLERWLAAVGDGTPDSMQAQR
                     LAGLARTQTLHATRCLAIARAFEQILAEMRINRVARPGDEQRISQRLVSPLGRLARET
                     IPASAEAMAPLREQVSPQLGLDAKAAQANVLAEMREILKSMKEWEGYREAVALLESTI
                     AAQAEVRSATLEAVAQQLDDILGLDDPPAESEGDAQ"
     gene            complement(7761..10235)
                     /locus_tag="PLU99_05395"
     CDS             complement(7761..10235)
                     /locus_tag="PLU99_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO16527.1"
                     /translation="MNLAILATATEIQQRGWELARCPQSWGRLVGVCLLAAICYAVVW
                     LYRRERRAGAGVRLRSILAAVRCLVMLALAAVWLEPVIATYYVRSLNGRVAVLVDASA
                     SMSVRDAEESPDASPQSSAENLPGSQGDSGNDETPQPQMTRFERVRRLLLANEADWLR
                     RLQERNDLSLYAFGDTTFPLNLPWSAPATSAPPTTQSGSSADSLATLAPSYPQTDLGQ
                     ALTAVLADMGEGPVAGVILISDGQVNRGTPAHEVAAYAERVRAPLFTIGIGSTHEPPN
                     VRVTNLSAPAVVPPDDPFEVRVELAATGAEPEELRVELTVEPSGGGEARPVSSRSVHV
                     GDSNAPVLFEVRPPVPGEYTYRARIQPLAGESVTDDNVRDTTVTVLDKQLRVLIVAGG
                     PSYEYRYLLPLLMRDKTVNVSAWLQSAEPRALRDGTTSITKLPREPEEILEYDVILLL
                     DPDPAELDAAWAVTVRRWVDEFGGGLLLQAGPQFASRLLRDTRVGEVVSILPITPDPD
                     ADVRLNAQGSYRTHPTVLQIPDTALTHPLLALQTDPAINRAVWNALPGVWWYLPVLRA
                     KPAATVLLEQPTAAQRSQYEAPVLMASQPVGAGRVAFMGFDTTWRWRGTAEEYYQRFW
                     VRAIRYLGQARRQGLSNRGTLVLDRESFNVGDYLKVEARVLDASFNPLQEEQITATLE
                     RSDGPPLEFVLQAVPERPGWYSGRVVLAQSGSALIRVPLLGGTVSAGPESLTRRIRVQ
                     QPDWEMRTLRLREELLAELAQQTGGQYVALRDAQRLPDLVPRAAERKPPQRAAVEPLW
                     DRAYVLLLIAGLLSIEWSLRRRNHLL"
     gene            complement(10232..>12424)
                     /locus_tag="PLU99_05400"
     CDS             complement(10232..>12424)
                     /locus_tag="PLU99_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16528.1"
                     /translation="PFVRPGLIAALLGGRGQVARIVILDDSASVSYRIGAAAEFDRLR
                     DATGRLLSWLHQEAPHDPVTVCLTSAGGIPMVQDALLSEADLADLRARIARLEPVEVP
                     ARPGRVIAALAARQLSAEIAGADYYVLSDFQQTDWLAGGADGRSVFAPLRAGGQAEPS
                     TSAVGEQASGQLTGAPRERPVRAVLVASGVQPRSNVALIDAQLARAQTIAGFPAVVRV
                     AIANYAMQTVSDIVLQVEIDGAPLPATPVGAIEAGQHKDVSLEVTFPEAGTHVLTLTL
                     GPVDSFRADDAHRQTVEVKSALAVLLVNGSPGADEYRDETFLLRNALAPPGPLSSGIQ
                     VDTVDPEQLRATELDRYDVVVLCNVPPPDEVAASALGTYVKAGGGLAIFLGSEVGDPA
                     EYNRTLYAGRAALLPAPLESLVSPPGEGGGVGLLRTEPHAVTAAFPAGSDVLSEYVHF
                     WRYYRCDEAGPREAEHAEPAAVVLARYADQEGSPAILERAVGRGRVLLFTSTADLEWN
                     DWARAPDGSFVVTMLELVHYLARRSDQPGAFVAGRPLYVTLSPQMYEPAAIFKSPAFP
                     NEPPFAASTGAPTDPDAPLVVPGPNATRLGTYTVDLTPRSDFGLTAPTVHPVCVNLDP
                     AESDLSVASPAALDAALAGIPHELVATADEFLQGAEHTRQELWPALLVLTVITLMSEQ
                     FLAWWFAKPRTGSGLATPVAAISGRRRLAGRLRRGRQAVVRAVRGNRP"
     assembly_gap    12425..12524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12532..13446)
                     /locus_tag="PLU99_05405"
     CDS             complement(12532..13446)
                     /locus_tag="PLU99_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO16529.1"
                     /translation="MPNSGRYLLPEVIARLRGLDLRARMVVAGVLSGMHRSVYHGQSV
                     EFAEHRPYVAGDDIRRIDWRLYGRKDRFFIKQYEEETNLRCNVLLDASRSMAYGSGPL
                     NKFDYAACLAAALAYLLVRQHDAVGLITFDHEIRRKLPATTGRPQLANVISLLEASQP
                     AGSTNVKILFHQLAEELRRRSLVVLISDLLADTQDVLAGLEHICYSGHELVLLHVMDD
                     DEWNFPFVENTLFEGLEEEQRLLADPQALRASYLEAVRDFCARVRAVCLKHRADYVPV
                     NTRQPLDAVLSGYLSGRSVRAAHGRTGR"
     gene            complement(13415..14599)
                     /locus_tag="PLU99_05410"
     CDS             complement(13415..14599)
                     /locus_tag="PLU99_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO16530.1"
                     /translation="MNRENEDVPPQAGAPDSLPAEAPHSLKQAAPAGGDDLELVRRLR
                     AAYAEIRAELAKVIVGQEAVLEQLLIALFARGHVILEGVPGLAKTLMISSLARCLSLD
                     FHRIQFTPDLMPSDITGTEVIQEDRRTGNRSFRFLPGPVFTNVLLADEINRTPPKTQA
                     ALLEAMQEAQVTVGGVRHPLNPPFFVLATQNPIEQEGTYPLPEAQQDRFMFKVLVGYP
                     PYADEFAIAERTTGGRQPELKPVLALEEILRLQEIVRRVPVAPSVIHYALNVVRSTRP
                     STARRDEDAGRAAGRVALPAAAAELIDRMVTFGAGPRAVQFLLLGAKVRALLIGRHHV
                     SREDLQALAHPVLRHRIITNYAAEAEGYDTDRIIEHILESLPAGRGDESANAQFRQVF
                     TS"
     gene            complement(14717..>15278)
                     /locus_tag="PLU99_05415"
     CDS             complement(14717..>15278)
                     /locus_tag="PLU99_05415"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOO16531.1"
                     /translation="NLVREDHLVVSIMAGVSTASIENSLPRIKARVVRTMPNLAAHVG
                     AGMAGVCPGRYASEADLLRAQRIFDASGKSVHIADESLMDAVTAVSGTGPAYYYFFTE
                     ALMEAAKRVGLSPQYANLLAKQTALGAARMMLESGEPPDVLRAKVTSPGGTTAAALTS
                     MRDNQIFEHIVAGVVAAFNRSRELGA"
BASE COUNT         2506 a         5280 c         4789 g         2603 t
ORIGIN      
        1 ggcggaccag cggttctggg cagagcaaat gatttcggcc gggcaatctg acgccccgca
       61 gtcggccgcg tcccagcctg gtgctcgcgc gagcgacgct atggcagggc gcgtgcgcag
      121 catagagcgg gcgatggctg agttggccgc tgcggtcgca gggcctctgg accgggagct
      181 gaccggccgt tacgcctgga agttgcgcaa tgcggagctg atcctggacg cgagccgtcc
      241 gcaggccggc ccgacggttc tcgtccgcca tgctgaagac cagatcattg gagtacataa
      301 cgtagtgctg ggggggcaat gcaaggaggt tctcgagctc tcggaatgcg actcgtacga
      361 tcccatgcag tcgcgaattg cggcgggctg gtgggccggc ggcgaggatc tggatgagga
      421 gaacacccga accttctggc gctgcgcgac caatggacat atggcggccg tcgcagtgca
      481 ggatgggctg tttggactgg gtttgggctg cgcgcggggc gcggacaaac tgtggcaccg
      541 cacgcttgcc gaatggggcg gaattgcggc gcatcctggc gaggcgctcg caggtggaac
      601 cgccggagtt tatgtggggt ggcgacagga tcgggtcgca ctattggact gggcggacgg
      661 gtcgccgcgc tgggagcgca gcctgcccgg tctgcggatc gatcaactct tgtcgctcgg
      721 cgagcgcttg atcatcgtcg ggcgtgacgg cgacgtcgtt tcggtcgacg caaactgcgg
      781 tgacgacctg cgtagtctat ccccagtggg cggcgcgccg cgcgccgtaa ccaccgccgg
      841 cgagacgctc gtggtttgga ccgaggatcg cgtaggcggc tatgacccgg ccactttcgt
      901 ctgccgctgg ttgcgtccga gccgcggcgt ggaagactat aaatgcgtgg ccggttcgaa
      961 ttgggtcttg tatcgcgacc agggcgaaac gcagtggggg ctgattgatg ccgacaccgg
     1021 cgacccggtg ttcgccgggc gatggggcgc gttcggcggc acgctgacgg ccgctgcgaa
     1081 agacggcgag cggctgctga tcgcggcctt ctcgcggaag gcggcttccg gcgacgcggc
     1141 gccgctggtg catttggcgg cgttcgacgc ggagagcgga gcgcagttgt gggaacggga
     1201 cgtgccgacc ctggcgcggg tcaatcagac gcaactgcag gcaagtgcgg acctgattcc
     1261 agtgctggtg gcgcgcagcg actacgagta caccgagacg cgcaattaca atgcacttgc
     1321 gctatgcgtt gtagacaagg aatcgggagc ggtgacggag ccggtctcga taatggacga
     1381 cttccgcggc acgcagggac gttgcgccgc ataccttctg gcaacgccca gcagaattat
     1441 cgtacaggcc aacgggacca tcgccgccta cggaccagtc agcggaagac agtgatgatc
     1501 ggtgcgtgta gagacatcgg gccgtccata atggcagcat tggccctgct ggtttgctgc
     1561 tacgcaacga catattccca ggtaacgccg gccggttcgg cagcgtcaga cactagccca
     1621 gccgagccgc ccgccgaccc cggttcggct ctgaatctgc cgccgcaact gccgcccgga
     1681 attacgccgg cggcggccga ttccattcag agaggcctgc gcgctctggt gagcctacag
     1741 aaccgggatg ggagctggcg caccggaggt tcttcgggcg ggtatccgac ggccatgacc
     1801 gcgctggccg ggctggcgct gctggcgggc gggaacacgc ccacgcaggg cgcgtatgcc
     1861 gagaacgtca gccgcggatt gtcctacctc ctgagcgtcg cgcggcgcga cggcctcatc
     1921 gcgagcgtgg aagaagaaag ccactgcatg cacgggcacg gctttgcgat gctcttcctg
     1981 agccaatgct acggcatgga ggaggacgcg caacggcggg cccaggtcgg cctggccttg
     2041 cagcgggccg tgcaactgac ggcgcgcagc cagagtgcgg ccggcggctg gctgtacacg
     2101 ccggattcca atggcgacga gggctccgtg acggtgacgc aagtgcaggc cctgcgcgcc
     2161 tgccgcaacg tgggtattgc ggtccccaag cgcgtgattg accaggccat gcagtacctg
     2221 gagcgctcgt tgaacgagga cggcggcatt cggtatcagg tgtccgatcg cgggccgagc
     2281 cggccggcga tcactgcagc ggcggtggcc tgctggtaca acgccggcct atacgacagc
     2341 ccgcacgttg cggcagcgct gcgcttcttg aagccgcgct tgacgcctga tccgaataac
     2401 gacgtacatc atttcgggca ctatttctac ggccaccttt acctggcgca ggttttgtac
     2461 cagacctcgc agcgcgattg ggaggagtac ttcccggcca tgcgcgacgc gctcattcgt
     2521 gctcaggagc gcgacgggac atgggagggc gacgcggttg gtccggtcta tggcacgtca
     2581 gtggcgctca taatcctgca gttaccttat aagaacttgc cgatcatgca gcgctgagaa
     2641 ctcgcagtcg tgagggcgag ggctcgaccc tgctcgaagc ttcgctttca agcagtggcg
     2701 cacgccgggt ggttcggaca gttacctctc ttcggccagt gagcggtagt actgctccac
     2761 cagttggcgg taacgcgagg ggaaacggtc acgcagcgaa tcgaggatgc gctcccgctg
     2821 cgccggcgga agctggcccc acatctggct cggatcggcc gcgggcgccc ggtgtaattc
     2881 gccgatcggc gccgccccgg actccggcgc ctccgatcgc tcgcgcggag ccccgggccg
     2941 cttgtcctgc gcggcgcgcc gctggcccgc gccgtcctgg cttttttcac gttcctgcgc
     3001 ttcttccaca agcttgtcaa gcagtgcaac cgcttcgtcc tggcgcgagc gcacgcgctg
     3061 cgtcgcatcc gcaaccgtga gccgtgcggc cgaataactc atgagcattg ccacctcgtc
     3121 cagcgtgccc cgctcgcgcc gctcgatctc gagccgcagt tgccgcgcgc ccgcctgcac
     3181 cggctcaggc gcgtccggga tatcggcctc caagctctgc agcgtttgca gggcctcagc
     3241 gaagcgcaga ttacttgctt cgcagtacgc gcgcatgaag cgcagatgac cggcgtacgg
     3301 cgtgtactgc tccaactccg gcccgccggc cgccggtccg gcggcgtttc gcaactcgtg
     3361 ctcagcttcc tccagcaggc cttgctcgat cagggcccgc acatagaggt attgcgcgtt
     3421 cgcggccaga aacgggtcgc ggctctcgcg cagcggccca gccagacgct gcatgtcgcc
     3481 atagcgctgc tcgtcaaacg cgttcaatgc gtcgcgataa tcagggtaca acaatcccag
     3541 cgcatccggc acgaagcttt gcggcatgct ctgctgataa ctttgccaag cctcgcgcac
     3601 cgcctccgcc gcacgggagt actcgggacc gagcgtgccg acgtgctcca gaaaggccct
     3661 gttcagccga tcgatttgcg ggcgggcggg ctgcgtcgtt gccgtctgcg tggccgcagg
     3721 ctgaccggcc gcgaccgtcg gccagagtat caatacgctg atcaccagac ccagcagcgc
     3781 ggacgcaaaa agtctcaggc gacggccgcg catctcactt cctttcattc atttgccgcg
     3841 ctatttccac caggcgccgt tgccgtgcgg ccaagcgcgc cagctccgcg gcggcctcag
     3901 gcgacgcggc gctgtcggcc gaaaggtccg cggtccgctg gttaatccgc atctgggcgg
     3961 agcgcaacag catcagttcc acggactcgg gcaggagcgc ctgccggccg ctttgaccgc
     4021 cctgatccgg cggttgcagc atgttctgga gctcctgctt tcggcggact tcaatggcac
     4081 ccacgacttc ctcgagcgcc gccagcacct cggcgattct ccgctgcgtc tgtactccag
     4141 tgtcggactg gtcgagccgg tcggcgatat cctccatgtt gcgcgccaaa tctccggtca
     4201 actccggcag cagcacggtc gtgccctcgc tgaccaggat gcgcgccacc tcggcacagt
     4261 ccgcggccac ggcgcgctgc gtctgcgctg tttccgacaa cttgagctgg tccggccttt
     4321 gccagccgcg cggcgccttc tcgtgcagag cgctcacgtc gtcgcggatc tgccgctggc
     4381 gcgccagcat gctcttgaag cgcgcgtcca gcgcagtgag cgtctcctcc agttcctcgc
     4441 gccgcacttg gcgcaacgca tcgtccagtt catccaacgc ccgctgcaac tgttccagag
     4501 ccgcttcttc ctcaggctcg gcggcacgca cgcgctgctc cgccagccgc tccgcagctt
     4561 tctgcatgct ccgctgggcg ttgcccactt gtcgctggcc cggcgtgccc ggccccgctt
     4621 taccgggttt cgggctgagc tccttttcca gtccgccggc cccttgcgca agggcgcgtt
     4681 gctgctgttc cagttcgtgc accttggcgc gctcggccag acgttgcgcc tgctcccgca
     4741 gacggcgcgc actgcggcgc aactgctcct cggcggcctg ctgatccaag ccggcctcgg
     4801 catcctcctg tgcttcgagt ttttctgatg cggcccgcat cttcgcggcc gagtcctgcg
     4861 tttcgccggc agactgctcc agagccacgg cgacctcagc ctggttcgtc tgctggcgca
     4921 gcttgtccag ttcagcggcg tgctgttccg cgctgtctgc gagttcctgc tgctcagcgc
     4981 cggtggcccg cttgcgcagc tcggcttgcc gctccgcgag gccctccagc gcatccgcct
     5041 gctcgcgcag ccgccgcgcc aactcgccat ccggcgccgc ggcctgggta cggtagagct
     5101 gctcgagctg ctgctgcatt aagctttgta ttccgcgttt tagccgttcg agtcgctcgc
     5161 gctcggcgcg cttgcgatcg aagtcgctgc cggtgtcttg gagctgctgt agaatctcct
     5221 ccaagtcggc caacaattcg ccctgggcgc gctccgccgc actgtagtcg cccgacttta
     5281 ccaagaacac gatgtcatcc agccgccgct tgatctgctt ctgcccgcac aggtcggtcg
     5341 cagtccgcag ccggtcggcc ttgtccggct cgctttcggc cagcacgcgc gccaacaggt
     5401 tcatgcggct ctcaagctcc tgcaaccgct ggcgtatgtc ctgctggcgc tgcagcagcg
     5461 ttggctgcgt gccggcgggc tgagccagag cgcttctcgg cgccacgtga accagcagcg
     5521 cgcagccgac tatcagcaag gcggcgcagc gtgccggcct gacgcagaac ccagaattcc
     5581 ctgcttccgc tctcattttc gctcgcttta ttgagcatct ccttcggatt cagccggcgg
     5641 atcatccagg ccgaggatgt catctagctg ttgagccacg gcttccaacg tggcgctgcg
     5701 cacttccgcc tgagccgcaa tcgtcgactc cagaagcgcc acggcctcgc ggtagccttc
     5761 ccattccttc atgctcttga gtatctcgcg catttccgcc agcacgttag cctgcgccgc
     5821 ttttgcgtcg agcccaagct gcggactgac ctgctcgcgc aacggagcca tcgcctcagc
     5881 gctggccggg attgtctcgc gcgccagccg ccccaagggc gacaccaagc gctggctgat
     5941 gcgctgttca tcgcctggcc tggcaacacg attgatgcgc atttcagcca gaatttgctc
     6001 gaaggcgcga gctatcgcca aacatcgcgt tgcgtgcagc gtctgcgtgc gggccagacc
     6061 cgccagccgc tgcgcttgca tgctatccgg cgtgccgtcg cccaccgccg ccaaccagcg
     6121 ctccagccca tccgccagcg ccgcctgagc gctgatcaaa cgttcaaatt cctggcgcaa
     6181 gcccaactcg cgccgcgcca cctcgatcag caggtcctcg ggcgagacga cccgcagcga
     6241 cgtcgccggc gtcgtcccgg cattcggctc gccgggcctt atgtcacgcg cctcaccacg
     6301 aatgcgcacg gtctgccccg ctgccgcgcc gccggccgcc atcatcagca cgaattcccc
     6361 cgtgtacttg cgggcggccg gttcgaagcc cggcgcgctt agcgttcgct ccggaccgtc
     6421 agccgtctgg accagcagcc gcgcggcgct gaggccgtac gtgtcttcgc atgtcaatct
     6481 caccggcagc tcggcctgcg gcgtcaccat ctcgcccacg ccggacaagt ccagcttcac
     6541 tgtcggcggc gcatccggcg ccactttgag cgtgtagctg accgggttgc ggctggtgag
     6601 attgtcgaag tcgagcaggg cgaagcgata aatgccggcc tgcggcgctg cccaccgcag
     6661 ccgaaccact gcctgcgcgt ccgccggatc gccccaacta actccgcaag gtgcgtcgct
     6721 ctcatcggcc gcaacgaaac gggcctgctg caccggctta ttcagcaggg cctcgatctc
     6781 cagcagtgag ccggccagta tttccagcac ggtctgctgc tcgatctcca gcggttccag
     6841 gcgcgtgtat tccggcggcg tgatgcgggc ccgcgtgtgc atgacgcgcg gccgctcaac
     6901 cgcttcgatg gtgaagacgc gcgtgcgttc gtcgccgccg gtgatgcgga actgaagctg
     6961 ctcagtcaac tggccgatcg acgccgtcag acgcgtgtca gcaaccagcg acatctcttc
     7021 gcgcccacga cgcccggacg gcgtccacca gtgcagcacc gccgagtccg gcacccgccc
     7081 ggtgatggcg gcgctgatct caattacatc gccgcgcgcc atccgccgcc gcccgtccgc
     7141 gtcaaagccc tccggccaca ggtgtgtgtg ctgcggccag gccacgtttt gcatgagcca
     7201 gttccgcgcc agccatggcc cggccaggct cgggaattcg cacacggcca gcgcgatgac
     7261 caggatcagc ccgagcgttc cggccgcctg gcgtgctgct cggcgatggt tcaatatgcg
     7321 ggaaaaatcg acgtctgcgc tggcgcggca agcctcagcc agcaccgtgt ttaccaattc
     7381 ggggcggcgg ccggacggca tttgttccgc caactcgacc gcgctcgaca gccgatcgtg
     7441 cagttcgggg ttggcacgat cgaccagccc ggccagcaga tgatcgggca tggggcggct
     7501 gagcgggaca atcagcttgc gccatacgac gaccgcccag tacagaagca gcacgccgct
     7561 gagaacgatt cgctgcgaaa gcggcagctt cagcgaccag tccaggaaaa actgcaccag
     7621 gctcgcggcc gccaccgcta ccgccagttg ctgcgcgccc gccaatgcca ggtacacaca
     7681 cgctcgccgg cgcaaccgcg ccaacggcgc cagcacgctc tcgcgcagtt cgctcgcaag
     7741 ttcttcaacc ggcgcgacgc tcacagcagg tgattcctcc ggcgcaacga ccattcgatg
     7801 gaaagcaaac cggcgatcag cagcagcacg taggcccggt cccagagcgg ctcgaccgcc
     7861 gcgcgctgcg gcggtttgcg ttccgcggcc cgcggcacaa gatcgggcag gcgctgcgca
     7921 tcgcgcagcg cgacgtactg cccgccggtc tgctgggcca gctccgccag caactcctcg
     7981 cgcaagcgca acgtgcgcat ttcccagtcc ggctgctgca cgcgtatgcg ccgcgtcagc
     8041 gattccgggc ccgcagacac tgtgccgccc agtagtggca cgcggatcag cgccgacccc
     8101 gattgcgcca gaacgacccg gccactgtac cagcccggac gttcgggaac ggcttgcagc
     8161 acgaattcca gcggcggtcc gtcagaccgt tccagtgtcg cggtgatctg ttcctcttgc
     8221 agggggttga acgacgcgtc caatacccgc gcttcgacct tcaaatagtc gcccacattg
     8281 aacgattcgc gatccaacac gagcgtcccg cgattgctca gcccctggcg ccgcgcctgg
     8341 cccaggtatc gaatcgcccg cacccagaac cgctggtaat actcctccgc cgtcccgcgc
     8401 caacgccagg tggtgtcgaa ccccatgaac gccacgcgcc cggcgccgac cggctggctg
     8461 gccatcagaa ccggcgcttc gtactgactg cgttgcgccg cggttggctg ctccagcaag
     8521 accgtggctg ccggcttggc acgcaaaacc ggcagatacc accacactcc cggcagggcg
     8581 ttccaaacgg cccgattgat tgcgggatca gtctgcagag ccagcagcgg gtgcgtcagc
     8641 gcagtgtccg gaatttgcag caccgtcgga tgcgtgcggt atgagccctg cgcattgagt
     8701 cgcacgtcgg cgtccggatc gggcgtgatc ggcagaattg aaaccacttc cccaacacgg
     8761 gtgtcgcgca gcagccgtga tgcaaactgc ggccccgcct gcaagagcaa tcccccgccg
     8821 aattcgtcca cccagcgccg cacggtcacc gcccaggccg catccagttc ggccggatca
     8881 ggatccagca ggagaatcac gtcgtattca agaatctctt ccggctcgcg gggcagtttc
     8941 gtgatactgg tcgtgccgtc gcgcagggcg cgcggctccg ccgattggag ccaggcgctg
     9001 acattgaccg tcttgtcgcg catcagcagc ggcaacaggt agcggtactc gtacgatggg
     9061 ccgccggcca cgatcagcac gcgcaactgc ttatccagaa ctgtgacggt tgtgtcgcgc
     9121 acgttgtcgt ccgtgactga ctcgcccgcc agcggctgaa tgcgagcccg gtaggtgtac
     9181 tcgccgggaa ccggcgggcg aacctcgaac agaaccggcg cgttcgaatc gcccacgtgc
     9241 acgctacgag aactgaccgg ccgcgcttca cctccgccgc tgggttcaac ggtcaattcc
     9301 acgcgcagtt cttccggctc ggcgcccgtt gccgcgagtt cgacgcgcac ttcgaatgga
     9361 tcgtcgggcg gaacaaccgc cggggcggaa aggttggtca cacgcacgtt cggcggctcg
     9421 tgcgtactgc cgatgcctat tgtgaagagc ggcgcgcgta cccgctcggc ataagccgca
     9481 acttcgtgcg cgggcgtgcc ccggttcacc tgtccgtcag aaatcagtat gaccccagcc
     9541 accggacctt cgcccatatc cgccaaaacc gcagtgagcg cctggcccaa gtccgtctgc
     9601 gggtacgaag gcgcgagcgt ggccagcgaa tcagcggatg atcccgactg cgtcgtgggc
     9661 ggggccgaag tcgccggcgc actccacggc aaattcagtg gaaaggtagt gtcgccgaag
     9721 gcgtatagac tcaggtcgtt gcgctcttgc agccggcgca gccagtcagc ctcatttgcc
     9781 agcaacaacc ggcgcacgcg ctcaaagcgc gtcatctgcg gctgcggcgt ttcatcattc
     9841 cccgagtcgc cttgcgatcc ggggagattt tcagcggaac tttgcggcga cgcgtccggg
     9901 ctttcctctg cgtcgcgcac cgacatgctg gccgatgcat caaccagcac cgccacgcgt
     9961 ccgttcagtg aacgcacgta gtacgtggca atcacaggtt ccagccagac tgccgccaat
    10021 gccagcatca ctagacaccg gactgccgcc aatatcgaac gcagccgcac cccggctccg
    10081 gccctccgtt cccggcggta tagccagacg accgcatagc aaatcgccgc cagcaggcaa
    10141 acgccgacca gccgccccca actctgcggg cagcgcgcca gctcccagcc acgctgctga
    10201 atttcagtcg ccgtcgccag gatcgccaaa ttcacggcct gtttcctcga accgcacgaa
    10261 cgaccgcctg ccgtccacgt cgcaatcgac ctgccagacg ccgtcgcccg gaaattgccg
    10321 ctacgggtgt ggcaagcccc gagcccgtcc gcggcttcgc gaaccaccac gccagaaact
    10381 gctccgacat cagtgtaatc accgtcaata ccagaagtgc cggccacaac tcctggcgcg
    10441 tgtgctcggc cccctgcagg aattcgtcgg cggttgcgac cagctcatga ggaatccccg
    10501 ccagtgccgc atcgagtgcg gcgggactgg ccacgctcag gtcggactcg gccggatcca
    10561 ggttcacaca taccgggtga actgtgggcg ccgtcaggcc aaagtcgctg cgcggggtca
    10621 agtcaacagt gtatgtgccc agtcgcgtcg catttggccc gggcacgacc agtggcgcgt
    10681 cggggtcggt cggggcgcca gtggacgctg caaacggcgg ctcgttcgga aacgccggtg
    10741 atttgaagat ggcggccggc tcgtacatct gcggcgaaag cgtgacatac agcggccggc
    10801 ccgcgacgaa tgcccccggc tgatcgctgc ggcgtgcgag gtaatgcacc agttcgagca
    10861 tcgtcacgac gaaactgcca tcgggcgcgc gggcccagtc gttccattcc agatccgcgg
    10921 tggacgtgaa caacagcacc cgcccgcgcc cgactgcccg ttcgagaatg gccgggctgc
    10981 cctcttgatc cgcgtaacgc gccagtacga ccgccgccgg ctctgcgtgt tctgcctcgc
    11041 gcggccccgc ttcatcgcac cggtaataac gccagaaatg cacgtattcc gaaagcacgt
    11101 cgctccccgc gggaaaggcg gccgtcacgg cgtgcggctc cgtgcgcagc agtcccactc
    11161 ctccgccttc gccgggcggc gaaaccagac tctccagcgg cgccggcagc agggccgccc
    11221 ggccggcata gagcgtgcgg ttgtactccg cgggatcgcc cacctcgctg ccgagaaaga
    11281 tcgccagccc gccgccggcc ttgacgtatg tgcccaaagc gctggcggcc acctcgtccg
    11341 gcggcggcac gttacacaac accacgacgt cgtaacggtc cagctccgtg gcgcgaagct
    11401 gctcggggtc gactgtgtcc acctgtatgc ccgaagagag cggcccgggc ggcgccaatg
    11461 cgttgcgcag gagaaacgtc tcgtcgcggt attcatctgc cccgggcgac ccattcacga
    11521 gcagtacggc cagtgcggat ttgacctcca ccgtttggcg gtgcgcgtca tcggcgcgaa
    11581 acgagtctac tggaccgagt gtcagcgtca gaacgtgcgt gcccgcttcc ggaaaagtta
    11641 cttcaagcga gacgtccttg tgctgccccg cttcaatcgc tcccaccggc gtcgcgggca
    11701 gcggcgctcc atctatctca acctgcagca cgatgtccga gaccgtctgc attgcgtaat
    11761 tggcgatcgc cacccgcacg accgccggaa aacccgcgat cgtctgcgcc cgcgcgagct
    11821 gcgcatctat caaggcaacg ttgctccgcg gctgtacacc cgaagcgacc aatactgctc
    11881 gcactggccg ctcgcgcgga gcgccagtca actggccgga agcctgttcg cccaccgcgc
    11941 tagtggaagg ctctgcctgg ccgccggctc tcagcggcgc gaacaccgat cgaccgtccg
    12001 cgccgccggc cagccaatcc gtctgctgaa aatcggatag cacgtagtag tcggcccccg
    12061 cgatctcagc ggacaattgc cgcgccgcca gcgccgcaat gacgcgcccc ggccgcgccg
    12121 gcacttcgac cggctccagg cgcgcaatgc gcgcccgcag atcggccagg tcggcttcgc
    12181 tcagcaacgc gtcctgcacc atcggaatcc ccccggccga agtcaggcaa accgtgaccg
    12241 ggtcgtgcgg cgcttcctgg tgcagccagg acaacagccg tccggtggca tccctgagcc
    12301 ggtcgaattc ggccgccgct ccgatgcgat aagagacgct ggccgaatcg tccaggatga
    12361 ctatgcgcgc cacttgcccg cgcccgccca gcagcgccgc gatcaatccc gggcgcacaa
    12421 agggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccgca ctcaccgtcc
    12541 ggtcctcccg tgcgccgcac gcacgctgcg cccgctgaga tagccgctca gcaccgcgtc
    12601 cagcggctgc cgcgtgttca ccggaacgta gtcggcgcga tgtttgaggc acaccgcccg
    12661 cacgcgcgcg cagaagtccc gcactgcctc gagatagctg gcccgcaatg cctgcgggtc
    12721 cgccagcagc cgctgctcct cttccagccc ctcaaacagc gtgttctcga cgaacgggaa
    12781 gttccattcg tcatcgtcca tgacgtgcag caggaccagt tcatgccccg agtaacagat
    12841 gtgctccaga ccggccagca catcctgcgt atccgccagc aaatccgaga ttaacaccac
    12901 tagcgagcgc cgccgcagtt cttccgccag ttgatggaac agtattttca cattcgtgct
    12961 accggccggc tgcgaggctt ccagtaacga aattacgttc gccagttgcg gccgcccggt
    13021 cgtcgccggc agtttgcggc ggatttcatg atcgaaggtg attaggccca ccgcatcgtg
    13081 ctgccgcacc agaaggtagg ccaacgccgc cgccaggcat gccgcgtaat caaacttgtt
    13141 caacggcccg ctgccgtacg ccatcgagcg gctcgcatcc agcagcacgt tgcagcgcag
    13201 gttcgtctct tcctcatact gcttgatgaa aaagcggtct ttgcgcccgt acagccgcca
    13261 gtcgatccgc cggatgtcgt cgccggccac gtacggccga tgctcggcga actccacgct
    13321 ctgcccgtga tatacgctgc ggtgcatgcc cgacagcacg cccgccacca ccatgcgggc
    13381 ccgcaggtcc agcccgcgca gacgggcgat tacttcagga agtaaatacc tgccggaatt
    13441 gggcattggc gctctcgtcg cccctgccgg ccggcaggga ctccaatatg tgctcgataa
    13501 tccggtcggt atcgtagcct tcggcttcgg cggcgtagtt ggtgatgatg cgatgtcgca
    13561 ggaccgggtg cgccagcgcc tgcaggtcct cacgcgatac atgatgccgc ccgatcagca
    13621 gcgcccgcac cttggcgccc agcagtagaa attgcacggc ccgcggcccg gccccgaacg
    13681 taaccatgcg gtcgatcagc tcggccgccg cggcaggcag cgccacgcgt cccgccgccc
    13741 ggccggcgtc ctcgtcgcgc cgcgcggtgc tgggccgcgt cgaacgcacc acgttcagcg
    13801 catagtgaat aacgctcggc gcaaccggca cgcgccgcac gatctcttgc aagcgcagaa
    13861 tttcctccag cgccagcacg ggtttcaatt ccggctgccg gccgccggtc gtgcgctccg
    13921 cgatggcgaa ctcgtcggca tacggcggat acccgaccag gaccttgaac atgaagcggt
    13981 cctgctgcgc ctccggcaac gggtaagttc cttcctgctc gatagggttc tgcgtggcca
    14041 gcacgaagaa cggcggattc aacgggtgcc gcacgccgcc cacggtgacc tgcgcctcct
    14101 gcatcgcctc gagcagcgcg gcctgcgttt tgggcggcgt gcggttgatc tcgtccgcga
    14161 gcagcacgtt cgtgaacacc ggcccgggca gaaagcggaa ggagcgattg cccgtccgcc
    14221 ggtcctcttg gatcacttcg gtgccggtga tgtcggacgg catcaggtcg ggtgtgaact
    14281 gaatgcggtg gaaatccagg ctcaggcagc gcgccaggct gctgatcatc agcgtcttgg
    14341 ccaaccccgg gacgccttcg agaatcacgt gcccgcgggc gaacagcgcg atcagcaact
    14401 gctccagcac cgcttcctgg ccgacgatca ctttggccaa ctccgcccga atttcggcgt
    14461 acgccgcgcg caatcgccgc accagctcca ggtcatcgcc tcctgcgggc gccgcctgct
    14521 tcaacgaatg aggcgcttcc gctggtagcg aatcaggcgc accggcctgc ggaggcacat
    14581 cctcgttttc acgattcata aatcacgcga aaccctccga agcctctcca tccgcttgga
    14641 ccgcacaggt acatgcgatt gtagaatacc gcgccgcgga tcacggtgca cttcccagac
    14701 gatgcgcttc cgacgcctag gcccccagtt cgcgactacg gttaaaggcc gccacgacgc
    14761 ccgcgacgat gtgctcgaag atctggttgt ctctcatcga ggtcagcgcg gccgccgttg
    14821 tgccgccggg ggaggttact ttggcgcgga ggacgtccgg gggctctccg gattcgagca
    14881 tcattcgcgc ggcgccgagg gcggtttgtt tggccagcaa attcgcgtat tgcggcgaca
    14941 ggccgacgcg cttggcggct tccatgagcg cttcggtgaa gaagtaatag taggccggcc
    15001 cggtgccgga gacggcggtg acggcgtcca tgaggctctc gtcggcgatg tgcacgcttt
    15061 tgccgctggc atcgaagatg cgctgcgccc gcagcaagtc cgcctcgctg gcgtagcgcc
    15121 ccgggcagac gccggccatt ccggccccga cgtgcgcggc caggttgggc atggtgcgca
    15181 ccacgcgggc tttgatgcgc gggaggctgt tttcgatgct ggcagtcgaa acgccggcca
    15241 tgatcgagac gaccaggtga tcctcgcgca ccaggttg
//
LOCUS       DAOSYS010000060        15045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3334_length_15045_cov_5.337959, whole genome shotgun sequence.
ACCESSION   DAOSYS010000060 DAOSYS010000000
VERSION     DAOSYS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15045
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3334_length_15045_cov_5.337959"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLU99_05420"
     CDS             complement(<1..993)
                     /locus_tag="PLU99_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOO16532.1"
                     /translation="MENALTEIVVLFGAALLAAWLMRILRAPAILGFLIAGILIGPSG
                     LNLIAQQDVHFFAELGLVLLLFVVGLELSVTPLVRSGPRLLLGAVLQLGVTALLGAVL
                     LYAFGLAGLLPAVILGSAAAISSTPVMINYFSDRGQTDSPASVTSTIISLVQDIGSIL
                     VLVLLPLLARTTEDWSAALLKTGLAFAGLVGSFVLGRLLLPILVRWFTQNGRSRELMT
                     LFAIVMACSGAWLAGEAGWSWGLGSFLAGLLLAQTDLRHQIRSEIMPFRNAFNAVFFI
                     SIGMLMQLSLFFEHTLVLIGAIVGLVVLKALIAGGAILVAGWPVRLAVIGGVALS"
     gene            1429..2187
                     /locus_tag="PLU99_05425"
     CDS             1429..2187
                     /locus_tag="PLU99_05425"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOO16533.1"
                     /translation="MSLHIQLPPEPEFAELISPQEWRTYAQIIEAAEARQVPIAVGGG
                     LAFSAYSQRWRSTKDIDFYIFHKDVEEMIDIVHGAGFQDYFEKLPYDRRWIYRAHRDG
                     IIVDLIWSMANYLAETDADWLSGGAEFQVSGTSVKLLPIEELIWAKLYVLQRDRCDWP
                     DLLNLIYVRAAQIDWERLLRRVGPDARLLGGVLSVFSWMCPERVQAIPEWVWQRLGLL
                     RPAECDQPPSAPEGGRAALLDRRPWFGPGIEHRA"
     gene            2210..>2997
                     /locus_tag="PLU99_05430"
     CDS             2210..>2997
                     /locus_tag="PLU99_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO16534.1"
                     /translation="MNNPQADLGTELRAIAKAGFDFLDLTLEPPAAASWRVDVRWLRS
                     ALQEHGLGVVGHTAYYLPIASPFEEVRRAAVSELKRCLMVFAELGVRWMNVHPDHYAP
                     HHDRKFFIERDLESLSELLDAARQVGVGLMLENLPGQFNSAAQLADLLEPLPELGLHL
                     DIGHAHLLTKQNTVEDILQAYGARLRHVHLHDNRGGDDDLHLPLGAGLIDVPRHIQTL
                     QKIGYDGTITLEVFTPNREYLIYSRDLLRHVWDRTAGQRRGSSD"
     assembly_gap    2998..3097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3285..4850)
                     /locus_tag="PLU99_05435"
     CDS             complement(3285..4850)
                     /locus_tag="PLU99_05435"
                     /inference="COORDINATES: protein motif:HMM:NF036968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16535.1"
                     /translation="MTPRSLDPLIPSSLSEIPAEDPAVYDMICQADTVGVFQIESRAQ
                     MSMLPRLKPRQFYDLVIEVAIVRPGPIQGGMVHPYLRRRAGLEAVEYPSEAVKGVLQK
                     TLGVPLFQEQVMRVAMVAAGFTAGEADQLRRAMSAWRRHSALEQFKIKLIRGMLDNGY
                     GREFAEQIYKQIHGFGEYGFPESHAASFALLAYASAWLKRYHPAAFCAALLNSQPMGF
                     YSPGQLIRDAIAHGVRVLPVDVNYSGWDCSLEFLEGREEKEGSRDLGIKGSRDAAVSF
                     SIPRSLDPLIPSLRLGLRLVRGLSAAKVAGIEAARREGPITSIRRLARRPDVARDTLV
                     RLAAADAFRSLGLQRRAALWQILALDEAEPPLFAELEPDEPAVPLPPMPLDETIFKDY
                     QTTGFSLNAHPLELIRSDLQGLSVTPNGRLRAIPNGTPVRVAGLVTVRQRPSTARGLV
                     FMTLEDESGNANLVIHPPIWERDQRVARACTALLAEGHVERQGEVVHVIVRRLHDLSH
                     RLASLTHRSRDFQ"
     gene            complement(4847..5317)
                     /locus_tag="PLU99_05440"
     CDS             complement(4847..5317)
                     /locus_tag="PLU99_05440"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOO16536.1"
                     /translation="MFRELIVWGKAVELAKVVYRQTQRMPAAERFGLTMQMRRAAVSV
                     ASNIAEGNARCTPADYLRFLGIARGSLAELETQILIAAGLGMMEEAPISPLLNETRRI
                     LQALINSLQRKEFATAPGPSKKLAKAPGQAKEFAETPGPAKKLASGKKLGRATS"
     gene            complement(5493..7142)
                     /locus_tag="PLU99_05445"
                     /pseudo
     CDS             complement(5493..7142)
                     /locus_tag="PLU99_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
     gene            7438..8403
                     /locus_tag="PLU99_05450"
     CDS             7438..8403
                     /locus_tag="PLU99_05450"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOO16537.1"
                     /translation="MAQGRVLVTRRIPPPAAEMLSGAGLTMDLIDSDDSASRSELLRR
                     LAGANGVIATLTDRIDAEALDAAGGSLKVVANFAVGYDNVDVPECTRRGVRVTNTPGV
                     LTDATADLAWALILAAARHVAAGDALVRHGDWKGWAPLQLLGLQLSGATLGILGAGRI
                     GSAVARRSVGFGMKVIYTDQRANPELERELEARRVDFNTLLAEADVLSVHIPLSAESR
                     HLIGAPQLARMKPTAVLVNTARGPIIDEAALVDALRSGRIAAAGLDVYENEPRLTPGL
                     SELSNVVLLPHLGSATTTTRQRMSQMTAENAIAVLSGREPPNPVN"
     gene            8505..9008
                     /locus_tag="PLU99_05455"
     CDS             8505..9008
                     /locus_tag="PLU99_05455"
                     /inference="COORDINATES: protein motif:HMM:NF038001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYExAFE family protein"
                     /protein_id="HOO16538.1"
                     /translation="MKGHIHYEAAFEDYLQAQQIPYVAVDEAKKATFRDAKLKSFDFI
                     VYSRGDHNWLVDIKGRRWVRRRPGSRPAWENWVTQADLEGLRQWQEVFGPGFGGLLVF
                     AYWLEPGSEPPPEVVHTFRAQRYVFAGVPLENYEEHARLRSPKWGTVSVPRRAFAQFV
                     RPIRDWL"
     gene            9366..12371
                     /locus_tag="PLU99_05460"
     CDS             9366..12371
                     /locus_tag="PLU99_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO16539.1"
                     /translation="MDGSGTSKEVPPKQDSISAWQDWRMQRFPQLMRRRVEDILLVSS
                     AYDAFSLQEDGLLTEALHADFTDLGLTHVPNITRVSTAEAALAAIREQHFDFIITMLR
                     VGDMDVFSFSRAVRQFAPDLPIVLLVSSNWELTRVSQQRDKLDVDDIYIWHGDTKIFV
                     AIIKCLEDRWNVEHDTRLARVGTIILVEDSIRFRSSLLPIMYTELVKQTRSVMADGIN
                     RMHRLLRTRARPKILVAETFEKGMEYYTRYHRYLFGVIADVSFPRGGKIDPQAGIDFI
                     RQVTADYPDMPALLQSSDLANRAVAESIGAKFLHKRSATLLEDVREFMLENFGFGDFV
                     FRMPNGEEVGRAFDLRSLPQVLARVPAEAIEFHARRNHFSNWLRARTEYELAQRLRPR
                     KVSEFGDVEALRQYLIRECNDALRANRRGMIEDFSRERFDAGSRFARIGGGSLGGKAR
                     GLAFVDALLTGTDLKREFPGISIQVPRSVVIGTDVFDEFLEANRLRMMALRTNDDEWL
                     TWAFLTAKLPEEVVRDLRAFLESVKGPIAVRSSSLLEDSQYHPFAGIYETHMIPNNSA
                     DGRVRLAQLCDAIKLVYASTFATSARRYLSMTPHRIEEEKMAVVLEPVVGARHGEYFY
                     PNFAGVARSYNYYPFGEMRPEEGIALVALGLGKTVVDGEAALRFSPAHPQVQPQLALG
                     EEFLKQSQRTFYAIDLMHPDGAVDRERLRGLVKLDIDQAQLHGTLAPLASVWSRENQA
                     FYDGLYSPGIPVVTFAQILKSDVFPLAAILRRLLRIGQAGMNRAVEIEFAVNLDANPK
                     ELAVLQLRPCMGSDGEIDQVDLGDHTGDEVLCHSPRALGNGVIDSVHDIVYVKPHAFD
                     ARRTAEIAGELAGLNQALLAANRSCMLIGPGRWGSSRPALGIPVNWGDISAARIIVET
                     TLDNFLVEPSQGSHFFQNLVSFGIAYLTVEPHAKQGSIDWNWLESQPAERETPFLRHI
                     RLPQPMEARIDGRSSRAVVLKRTRRVALE"
     gene            12438..13469
                     /locus_tag="PLU99_05465"
     CDS             12438..13469
                     /locus_tag="PLU99_05465"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO16540.1"
                     /translation="MQRRLMGINEFAAIGLLFAIWVGAQFSAAAQTPAGVPSGDRPAE
                     PQAQTAPVSDRSADIFDSAADAKAQIATALEAAQREHQRVLLLLGGNWCTWCHRLHTL
                     FKENKDVARLLKSEYQLVPVDVGRFDKNTDIVSGYGLELKKTGVPFLVVLDSDGKVLA
                     KQETGSLEKGQAHDPDKVLAFLTKWKAEPRDAETVVTQALARATAEHKSILLRLQAPW
                     CVWCRRLEAFLSRPEIEPLIGRDFVDLKIDMERMTHAKEVAARFCEKDAPGMPWLAVL
                     DAEGKVVVTSDGPDGNIGCPVAPGEISHFMEMLRKGAKKLSAGDLQKIEDLLKEAAAK
                     FKPAATTQP"
     gene            13790..>15045
                     /locus_tag="PLU99_05470"
     CDS             13790..>15045
                     /locus_tag="PLU99_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOO16541.1"
                     /translation="MKIGIDARTIFSPQPRGTGRNLFDAYRLIPALRPDWTFHLYHQR
                     PAAACPLVCGAAEADRPEESAWPANVALRRIDIPGDRLNLWFQLRLPVAAWRDRLDLL
                     HLPANAAPAWCPVPTVVTVHDLVPLKIADELPPPARERFRRGVLRAARTARHIITPSA
                     TTRDELCGEFGVCADRVTVIPWAADRLIAGMSDATSSSAFHAVRRRYELHRPWMLNFS
                     GPTRRKNAAGLLAGFAQLPAEIRAVCDLVLVGCATSEFQAELAAMAQTLGISEHCRFL
                     AFVPHADLPYLLGGARALLMPSLAEGFGLPILDAFACGVPVLTSACSSMPEVAGDTAL
                     YCDPVSPASIAAGMLQILQRQVATALVARGRQRVQQFTWEATATAMCQVYERCLLETG
                     RLQAPYPLPAGRDERGHATQAAGAGAA"
BASE COUNT         2747 a         4647 c         4896 g         2655 t
ORIGIN      
        1 cgacaacgcc acgccgccga tgactgccag gcgcaccggc cagcccgcaa ccaggatggc
       61 accgcccgcg atcagggctt tcaacaccac caggccgacg attgccccga tcagcaccag
      121 tgtgtgctcg aaaaacaggc tgagctgcat cagcatgccg atggagatga agaataccgc
      181 gttaaacgca ttgcggaagg gcatgatctc cgaccgaatc tggtgccgca gatcggtctg
      241 ggccagcagc agccccgcca gaaacgaacc caagccccag gaccacccgg cctcccccgc
      301 cagccaggcg cccgaacagg ccatgacgat cgcgaacagc gtcatcaatt cgcggctgcg
      361 gccgttctga gtgaaccagc gtacgaggat cggcagcagc aaacgcccga ggacgaagga
      421 gcctaccagc cccgcgaagg ccaggccggt tttcagcagt gcggcggacc agtcctcggt
      481 cgtgcgcgcg agaaggggca gcaggaccaa caccaggatc gagccgatgt cctgcacgag
      541 cgagatgatg gtcgatgtca cgctcgcggg cgaatcggtt tgcccgcgat cactgaagta
      601 gttgatcatc accggcgtgc tgctgatagc cgcggccgag ccgaggatca cggccggcaa
      661 cagtccggct agtccgaacg catagagcag cacagcgccc agcagggccg tgacgccgag
      721 ctgcaagacc gcccccaaca gcagccgcgg cccgctgcgc accagtgggg tcactgaaag
      781 ttccagcccg actacgaaca gcagcaggac caggccgagt tcggcgaaaa agtgcacgtc
      841 ctgctgggcg attaagttca aacctgacgg gccgatcagg atgcctgcta tgagaaaacc
      901 gagaatggcg ggcgcacgca ggatgcgcat caaccacgcc gccagcaggg ccgcgccgaa
      961 aagaaccacg atttcggtaa gcgcgttttc catatcgggt gctcgacggg gtcagactat
     1021 acgacgaaag gggcgtacat ggccagacct gcgcgggggg cacagcgcga gcgcctccac
     1081 tcgcccaaat ctccttcttg aagctcgcga ttgatcgcgc ggtggcggaa acccgccggc
     1141 acgtgccgtc gtcgaactcg accccataac gtcgcagccc gccccgaacg cttgcagagc
     1201 gcaacgttca gcccggcggg gcgggggccg acgaccataa cgtttatggc tcgtggattt
     1261 gcggcgtctc ctggcggccc ggttcagttc ggcaccaggc tgcgcgcacc gcgtaacaga
     1321 cttatgaacg ccggctaaaa tccggctcag gggcggcgac gaatttcacg gcgccgcatg
     1381 gcaaaacctc gcagcggcgc taaaacctgt cagaccaagg attggagcat gagtttgcac
     1441 atacagttgc cgcccgagcc cgagtttgcc gagctgatct cgcctcaaga atggcgaact
     1501 tacgcgcaga ttatcgaggc cgccgaggcg cgccaggtcc cgatcgcagt ggggggcggg
     1561 ctggcattct cagcctattc gcagcgctgg cgcagcacca aggacataga cttctacata
     1621 ttccacaagg acgtggagga gatgatcgac atcgtgcacg gggccgggtt tcaggattat
     1681 ttcgagaagc tcccttatga ccggcgctgg atttaccggg cccaccgcga cggcatcatc
     1741 gtggacctga tttggagcat ggccaattac ctggctgaaa ccgacgcgga ttggctgagc
     1801 ggcggcgcgg agttccaggt gagcggcacg agcgtcaagc tgctgccgat cgaggagctt
     1861 atttgggcca aattatacgt gttgcagcgc gatcgctgtg actggcccga tctgctgaac
     1921 ctcatttacg tccgggcggc gcaaattgat tgggaacggc tcctgcggcg ggtcgggccg
     1981 gatgcgcggc tgctgggcgg cgtgttgagc gtgttctcct ggatgtgccc ggagcgcgtc
     2041 caggccattc cggaatgggt gtggcagcgc ttgggacttt tgcgtccggc agagtgcgac
     2101 cagccgccaa gtgcgcccga gggcggccgg gctgcgttgc tggaccggcg gccgtggttt
     2161 gggccgggca ttgagcaccg cgcataggtg agacgtggaa atcggggcga tgaacaaccc
     2221 gcaagccgac ctgggcacgg aactgcgcgc catcgcgaag gccggcttcg actttctgga
     2281 tttgacgctg gagccgccgg cggccgccag ttggcgcgtc gacgtgcgct ggttgcgttc
     2341 ggccctgcag gaacacggcc tgggcgtggt cgggcatacc gcgtactacc tgccgatcgc
     2401 cagcccattt gaagaggtgc ggcgggccgc agtgagcgaa ctgaagcgct gcctgatggt
     2461 gttcgccgag cttggcgtgc ggtggatgaa tgtacacccg gatcactatg ccccgcatca
     2521 cgatcgcaag ttcttcatcg agcgcgattt ggagagcctg agcgagctgc tggacgccgc
     2581 ccggcaagtc ggcgtggggc tgatgctcga gaatttgccc ggtcaattca actcggccgc
     2641 gcagttggcc gacctgctgg aacccctgcc ggaactggga ctgcacctgg acatcgggca
     2701 cgcccacctg ctgacgaagc aaaacacggt tgaagatatt ttgcaggcct acggcgcgcg
     2761 gctgcggcac gtccatcttc acgacaaccg cggcggggac gacgacctgc acctgccgct
     2821 gggggcgggg ctgattgacg ttccgcgcca catccagacg ctccagaaaa tcggctacga
     2881 cggcactatc acgctggagg tctttacccc gaaccgcgag tatttgatct acagccgcga
     2941 tttgctgcgg cacgtctggg accgtacggc agggcagaga cgcgggagta gcgactannn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgc tgcggcacgt ctgggaccgt
     3121 acggcagggc agagacgcgg gagtagcgac taagtcacag ttcttagttc ttagtcgtta
     3181 gcgccggcgt cctgcatctt tcccaccttc ccaccttccc acgttcccat tttcccacgt
     3241 tcccaccctc gccgtgcgct tgagtgcgac gctccgggcg cgccctattg gaagtcgcgc
     3301 gaccgatgcg tcaggctggc cagccggtgc gatagatcgt gcaagcggcg cacgattacg
     3361 tgtacgacct cgccctggcg ttcgacatgg ccttcggcga gcagagcagt gcaagcccgc
     3421 gcgacccgct ggtcgcgttc ccaaatgggc gggtggatta ccaggttggc gttgccgctt
     3481 tcgtcctcca gcgtcatgaa gactaagccg cgggccgtac tggggcgctg gcggacggtg
     3541 accaaaccgg cgacgcggac cggggtgccg tttgggattg ccctgagccg gccgttgggc
     3601 gttacgctca aaccttgtag gtcgctgcgg atcagttcca gagggtgggc gtttaacgaa
     3661 aagccggtag tctgatagtc tttgaagatg gtttcgtcca ggggcatggg gggcaagggc
     3721 accgcgggtt cgtcgggctc caactcggcg aacagcggcg gctcggcctc gtccagtgcc
     3781 agaatttgcc acagcgccgc gcggcgctga agcccgagtg aacggaaagc gtcggccgcc
     3841 gccagtcgca ccagcgtgtc gcgcgccacg tccggccgcc gcgccagccg ccggatggag
     3901 gtaatcgggc cttcgcggcg ggccgcttca atgccggcca ccttcgcggc actcagtccg
     3961 cgcaccaggc gaaggcccag gcgaagagaa gggatcaagg gatctaggga tctagggatc
     4021 gagaaggaga cggccgcgtc tcttgatccc ttgatcccta gatccctaga tccctccttt
     4081 tcttccctgc cctccaaaaa ctccaaacta caatcccacc cactgtaatt cacatccacc
     4141 ggcagaacgc gcacgccgtg tgcgatcgcg tcccgtatca actgccccgg actgtagaat
     4201 cccatgggct gtgaattcaa caatgcggcg cagaatgcgg ccggatggta acgcttgagc
     4261 caggcggacg cataggccag cagtgcgaac gaggcggcgt ggctctcggg aaagccgtac
     4321 tcgccgaagc cgtggatctg tttatagatc tgctcggcaa actcgcgccc atagccgttg
     4381 tcgagcatgc cgcggatgag cttgatttta aactgctcca gcgcgctatg gcggcgccag
     4441 gcggacatgg cgcggcggag ctgatcggcc tcaccggcgg taaagcccgc ggccaccatc
     4501 gccacacgca tgacttgctc ctggaaaagc ggcacgccca gagttttctg cagaacgcct
     4561 ttaacggcct cgctgggata ctcgacggcc tccaatcccg ctcgccggcg caagtagggg
     4621 tgaaccatgc caccttgaat gggtcccggc cgcacgatcg cgacctcaat aaccagatcg
     4681 tagaactggc gcggcttcag gcgcggcaac atgctcatct gggcgcggct ttcaatctgg
     4741 aagacgccga cggtgtcggc ttggcagatc atgtcgtaaa cggcagggtc ctcggctggg
     4801 atttcggaca gggaagaagg gatcaaggga tcgagggatc gaggggtcaa gaggttgcgc
     4861 gcccgagctt tttgccggaa gccagctttt tggccggccc aggcgtttcg gcgaactctt
     4921 tggcttgccc aggcgccttg gcgagctttt tggagggccc gggcgctgtg gcaaattcct
     4981 ttcgctggag actgtttatc agtgcctgca gaatccgcct ggtttcgttg agcaaggggc
     5041 ttatgggtgc ctcttccatc attccgagcc cggctgcaat gagaatttga gtctccagtt
     5101 cggccagcga accgcgcgcg attcctaaga agcgcaagta gtcggcgggc gtgcagcgtg
     5161 cgttgccctc ggcgatattg ctcgcgaccg acactgccgc gcgccgcatc tgcattgtca
     5221 ggccgaaacg ctccgcggca ggcatgcgtt gcgtctgccg gtaaaccact ttcgccaact
     5281 cgaccgcctt cccccagacg atcagttccc gaaacatcat cctctccggg cgcgtttccg
     5341 attgctcgtt catgttttcg ctcctctgtc ctctcgttcc cgtgttcctc ttcttctgga
     5401 tcccttgatc cctcgatccc ttgatccctc attatactaa gcaacctaaa gcacttattg
     5461 atcgccgtaa gcatacccag cgccagacag tccactttca ataggccgag cgcgtctata
     5521 tcatctttgt cccattcgat gaaagtgcgc ccgggcatcg ccccattctc cagtgggaca
     5581 atttcgcaca atggagtctc ggtgatgaca aaaccgccga catgctggga aagatgacgc
     5641 gggaaaccca gcaattgcac tacgagccga atcaacatct gaaccgtgcg gtcttccggt
     5701 ttcagcccgg ccgcggccaa agcgtcgggc ggaatggtgt cgatgtccca ccagtccagc
     5761 gatttggcca gggcgtccac gcgatccagt gacagcccca ataccttgcc cacgtcgcgc
     5821 gccgctgaac gcgggcgata ggtaataact tccgcggtaa tgccggcccg ctcgcggccg
     5881 tacttgttgt agatgtactg aaagacctct tcgcgccgct cgtgctcgaa gtctatatct
     5941 atgtcaggcg gctcgttgcg ctcgcggctc atgaaacgct cgaacaacac gtcgatacgg
     6001 ttcggatcga cggccgtcac gcccagacaa tagcaaacgg ccgaattcgc cgccgatccc
     6061 cggccctggc aaagaatgcc gcggctgcgg gcgaagcgca ccaggtccca gacggtcagg
     6121 aagtaatgct cgtacctcag ttcggcgatc aacgccagtt cgcgctcgag ctggctgcgt
     6181 acaccatccg gcagcccgtc ggggtaacgc gcggccgccc cggcccacgt caggtcggcc
     6241 agatacttca tcgccggcac gcccggcggg cacaactcgt gcgggtactc gtagcgcaac
     6301 tcgtccaagg tgaacgtgca gcggttggcg atctcgatgg tgcgcgccac cgcgtcttcg
     6361 tagccggcgt aacgccgcgc catctcgtcg cgcggccgca agtggcgttc ggcattggcg
     6421 aacaggcggc tgccggcttc ggccagcgtg cattggtggc gaatgcaggt gagcacgtcc
     6481 tgcaaatagc ggcgttgagg ggtgtggtaa tgcacgtcgt ttgcggcgac cagcgggatt
     6541 cgcaggcgtc gcgacaggtc cgccaggtac gccagccgaa ctgagtcggg cacgtcgtag
     6601 gtcagctcgg ccgccaggta gaggcggtca gcgaaggcgt cgcggtatgt ttcgaagaga
     6661 tccagggatc gacggatcaa gggatcgtga aggttcgcgg gcgggagtgc tatcccgatc
     6721 aggccctcgc ataggtccag gacgtcgcgg acggtcagct cgctctggcc tttgggcatg
     6781 cgcaggcggc cgcgcgtgat caggcgcgcc agccgcccgt aggccgggcg gtcggtcgcc
     6841 agcaggacga gcggcggtcc gtccacgggc gtgatttccg cgccgatgag caggtgaagc
     6901 tggcgctctt tggcggccgt gtgggcccgc acgatgccgg ccagcgattc gtgatcggtt
     6961 atggcgagcg cggcatagcc ggcggccgcg gcctgttcga ccaattcctc gggatgcgag
     7021 gcgccgcgca ggaaggagaa gttcgacagg cagtgcagct ctgcgtaggc cggttccgcc
     7081 ggggatgcgc cgttggcatt cacgcaagtc tgaggccggg ctatcgaaac ctgacgagcc
     7141 atttacgttg gccggcggcc cgcaaaaccg ccgctcgccc gcgacacgga gacgccgagc
     7201 caccttatga tatcacccta acagcagcca aacaagcggc ctttactcaa gcaatcggac
     7261 gcgcgtgcca gggtgataaa gtgataaagt gatagggtga taaaggggac ccgcggatcg
     7321 tcccctatca ccctatcact ttatcacctt atcactcatg gcgtgcttgc ggcggggggt
     7381 ttgaaatggt atacggcgag gacttccacg atttgacggg aaaatttgga ggagctgatg
     7441 gcgcaagggc gagttctggt cacgcggcga ataccgccgc ccgcggccga gatgctgagc
     7501 ggcgcggggc tcacgatgga cttgattgac agcgacgact ccgccagccg aagcgagctc
     7561 ttgcggcgac tggccggcgc gaatggggtg attgccacgc ttaccgatcg gatcgatgcc
     7621 gaggcgctcg acgcggccgg tggctcgctg aaagtagtcg cgaacttcgc ggtcggctat
     7681 gacaacgtag acgtgcccga atgcacgcgc cgcggcgtac gtgtgacaaa cacgcccggc
     7741 gtactgacgg acgcgaccgc cgacctggcc tgggcgctga tcctggcggc ggcccggcac
     7801 gtggcggccg gcgatgccct ggtacgccac ggcgattgga aaggctgggc tccgctgcag
     7861 ttgctcggtt tgcagctctc gggcgccacg ctgggcattc tgggcgccgg gcggatcggc
     7921 agcgccgtgg cgcggcgctc cgtcgggttc ggcatgaaag tgatctacac cgaccagagg
     7981 gcgaatccag agctggaacg cgaactggag gcacggcggg tcgactttaa tacgctgctt
     8041 gccgaagcgg atgtactatc ggtacacatt cccctgtcgg ccgagagccg gcacctgatc
     8101 ggtgcgccgc aactggcgcg aatgaagccg accgcggttc tggtgaacac ggctcgcggg
     8161 ccgatcatcg atgaggcggc gctggtcgac gcgctgcgca gcggccgcat tgcggcggcc
     8221 ggactcgatg tatacgagaa tgagccgcgg ctgacgcccg gattgagcga gctgtccaac
     8281 gttgtcctgc ttccgcattt gggcagtgcg accactacta cacggcagcg gatgtcacaa
     8341 atgacggccg agaatgcgat cgcggtcttg agcgggcgcg agccgccgaa tccggtaaac
     8401 taaagccgca gtgaaacgcg ggtctgccga cccggacttc aatgaaatgt cgaatccgcg
     8461 gacttggtgt acggtcgtct gcggagccgg cctacggtgc ggctttgaaa gggcacattc
     8521 attacgaggc ggcgttcgag gactatttgc aggcacagca gattccctac gtcgccgtgg
     8581 atgaagccaa gaaagccaca tttcgcgatg cgaagctcaa gagttttgac ttcatcgtgt
     8641 actcgcgcgg cgaccacaac tggctggttg atatcaaggg ccgccgctgg gtgcgccgcc
     8701 gccccggcag caggccggcc tgggaaaact gggtgacgca ggcggacctg gaaggcctgc
     8761 ggcaatggca ggaagtattc gggccgggct tcggcggatt gctggtattc gcatattggc
     8821 tggagccggg cagcgaacct ccgccggaag tggtgcacac atttcgcgcg cagcgctacg
     8881 tcttcgccgg cgtgccgctg gagaattacg aagaacacgc acgcttgcgt tcgccgaaat
     8941 ggggcacggt gagcgtgccg aggcgcgcgt ttgctcagtt cgtacgccca atccgcgact
     9001 ggctgtagag catgcccggc aacggggcac cgtgcccgag caaagcgtgt catgccaaag
     9061 gttgggtgcc atgccaagcg acgcgtgcca tgcccaagcg ttgggtccat gcccaagccg
     9121 cgtagcggcg cgggcatgcc cccgcggcag cggcgccaga tacggaagcg attggcaccg
     9181 gcatgcccgc gcttacagct cgggcatggc accctcgaac gcccaccctc gctaccccaa
     9241 atgcctccct tgccggcgtc tcccgcttcg gccgcaatcc gcgtacaatc ggccgcgaat
     9301 cggctgagcg gcagggccgg agcctgggtg agcgcttccg gccgcggata tggcagacgg
     9361 gagttatgga cggcagcggt acaagcaagg aagtcccccc caagcaggat tcaatctccg
     9421 cctggcagga ctggcgcatg cagcgctttc cgcaactcat gcggcggcgg gtcgaggaca
     9481 tcctgctggt ctccagcgcc tatgacgcat tcagcctgca ggaggacggc ctgctcaccg
     9541 aggccctgca cgccgacttc accgatctgg gcctgaccca tgtgccgaac atcacccgcg
     9601 tctcgaccgc cgaggccgcg ttggccgcaa tccgcgagca gcatttcgac ttcatcatca
     9661 ccatgctgcg cgtcggcgac atggacgtgt tcagcttcag ccgggccgtg cggcagttcg
     9721 cgcccgacct gcccatcgtg ctgcttgtct ccagcaactg ggagctgacg cgcgtcagcc
     9781 agcagcgcga caagctggac gtggacgaca tttacatctg gcacggcgac acgaagatct
     9841 tcgtggccat catcaagtgc ctcgaagacc gctggaacgt ggagcacgac acgcgcctgg
     9901 cgcgcgtcgg cacgatcatt ctggtggagg actcgatccg ctttcgctcg tccctgctgc
     9961 ccatcatgta caccgagctg gtcaagcaga cgcgctccgt catggccgac ggcatcaacc
    10021 gcatgcaccg gctgctgcgc acgcgggccc ggcccaaaat cctggtggcc gagacgttcg
    10081 aaaagggcat ggagtactac acccgctacc atcgctacct gttcggcgtc atcgccgacg
    10141 tgagtttccc gcgcggcgga aagatcgatc cgcaggccgg aatcgacttc atccgccaag
    10201 tgacggccga ctaccccgac atgccggcgt tgctgcaatc ttcggatctg gccaaccggg
    10261 ccgtggccga atccatcggg gccaagttcc tgcacaagcg ttccgccacg ctgctggaag
    10321 acgtgcgcga gttcatgctc gagaacttcg ggttcggcga tttcgtcttt cgtatgccca
    10381 atggggagga ggtcggtcgg gcgttcgatc tgcgctccct gccgcaggtg ctggcgcgcg
    10441 tgccggccga ggcgatcgag tttcacgcgc ggcgcaacca tttctccaac tggctgcggg
    10501 cccgcaccga gtacgagctg gcgcaacgtt tgcggccgcg aaaggtctcc gagttcggcg
    10561 acgtcgaggc tttgcggcag tacctgatcc gcgagtgcaa tgatgcgctg cgtgccaacc
    10621 ggcgcggcat gatcgaggac ttttcgcggg agcgtttcga cgccggcagc cgtttcgccc
    10681 gtatcggcgg cggctcgctg ggcggcaagg cgcgcgggct ggcgtttgtg gatgctttgt
    10741 tgacgggcac ggacctgaag cgcgaattcc cgggcatcag catccaggtg ccgcgttccg
    10801 tggtcatcgg cacggacgtc ttcgacgaat tcctcgaagc caatcggctg cgcatgatgg
    10861 ccctgcggac caatgacgac gaatggctca cgtgggcctt cctgacggcc aagctgccgg
    10921 aagaggtggt gcgcgacttg cgggctttcc tcgaatcggt caaagggccg atcgcggtgc
    10981 gctcgtccag tttgctcgag gattcgcagt accacccgtt cgcgggaatc tacgaaacgc
    11041 acatgatccc caataacagc gccgatggcc gcgtgcgcct ggcacaactg tgcgacgcga
    11101 tcaagctggt ctacgccagc acgtttgcca cctcggcccg ccgctatctg agcatgacgc
    11161 cgcaccgcat cgaggaagag aaaatggcgg tcgtgctgga gccggtggtg ggggcgcggc
    11221 acggcgagta cttctatccc aactttgcgg gggtggcccg ctcatacaac tattacccgt
    11281 tcggtgagat gcggcccgaa gagggcatcg ccctggtagc gctcgggctg ggcaagacgg
    11341 tggtggacgg cgaagccgcg ctgcgttttt ccccggccca cccgcaggtc cagccgcagc
    11401 tcgcgctggg cgaggaattt ctcaagcagt cgcagcgcac cttctacgcc atcgacttga
    11461 tgcacccgga cggcgcggtc gaccgcgagc ggctgcgcgg tttggtcaaa ctggatatcg
    11521 accaggctca acttcacggc acactggccc cgctggcgtc cgtgtggagc cgcgagaatc
    11581 aggctttcta tgacggcctg tatagtcccg gcatcccggt cgtgactttc gcccagatcc
    11641 tcaagtccga cgtgttcccg ttggcggcga ttctgcggcg gctgctgcgg atcgggcagg
    11701 cgggcatgaa tcgcgcggtg gagatcgagt tcgcggtgaa tctggacgcc aaccccaagg
    11761 aactggcggt gctgcaactg cgaccgtgca tgggcagcga cggcgaaata gaccaggttg
    11821 atctgggcga tcatacgggc gacgaagtgc tttgccacag cccgcgggcg ctgggcaacg
    11881 gcgtaatcga ctcggtgcac gacatcgttt atgtcaaacc gcacgccttt gacgcccggc
    11941 ggacggccga gatcgccggc gaactggccg ggctgaacca ggcgctgctg gccgcgaacc
    12001 ggtcttgcat gttgatcggt ccggggcgtt ggggctcctc gaggccggcg ttgggcattc
    12061 cggtgaactg gggcgacata tccgcggccc gcatcatcgt tgagaccacg ctggacaact
    12121 tcctggtcga accttcgcag ggcagccatt tcttccagaa cctggtctcg ttcgggatcg
    12181 cttacttgac ggtcgagccg catgcgaagc aagggtcaat cgattggaat tggttggaaa
    12241 gccagccggc cgagcgcgaa acgccgttcc tgcggcacat ccgcctgccg cagccgatgg
    12301 aggcgcgcat cgacgggcga agttcacggg cggtggtgct gaagcgcacg cggcgcgtgg
    12361 cgctggaatg aactagcgga gcctcgggta cgggtgctta caacggccgg agaaccgccg
    12421 cgccggaaag gaacgcgatg caacgcaggt tgatgggaat taatgagttt gccgccatcg
    12481 gtttgttgtt cgcgatttgg gtaggtgcac agttctcggc tgcggcgcag accccagccg
    12541 gcgtgccgtc cggtgaccgg cccgcggaac cgcaggctca aacggcgccg gtatctgatc
    12601 ggagcgccga cattttcgac tcggccgcgg acgccaaagc ccaaattgcc acggccctcg
    12661 aagcggcgca gcgcgaacac cagcgcgttt tgttgctttt gggcggaaac tggtgcacct
    12721 ggtgtcaccg gctgcacacg ttattcaagg aaaacaagga tgtcgcgcgc ctgctgaaat
    12781 ccgaatatca gctcgtgccg gtcgacgtcg gccggtttga caagaatacc gacattgtct
    12841 ccggctatgg gctggagctg aaaaagaccg gcgtgccgtt cctggtcgta ctcgactcag
    12901 acggcaaggt gctcgccaaa caagagaccg gcagcctcga gaagggccag gcccacgatc
    12961 cggataaagt gctggcgttc ctgacgaaat ggaaggccga gccgcgcgac gcggaaacgg
    13021 tggttacgca agcgctggcg cgggcgaccg ccgagcacaa gtccatcctg ctccgcctcc
    13081 aggctccctg gtgcgtctgg tgccgccggc tggaggcgtt tctgtcgcgg ccggagatcg
    13141 aaccgctgat cgggcgcgac ttcgtggatt tgaaaatcga catggagcgc atgacgcacg
    13201 ccaaggaagt tgcggcccgt ttctgcgaga aggatgcgcc cggcatgcct tggttggccg
    13261 tgctggatgc ggagggcaag gtggttgtga cgtccgacgg tccggacggc aatatcggct
    13321 gcccggtcgc gcccggcgag atcagccatt ttatggagat gttgcgcaaa ggggcgaaga
    13381 agctctctgc gggcgaccta cagaaaatcg aggatttgct gaaggaagcc gccgcaaaat
    13441 tcaaacctgc agcgactact cagccgtgaa gccgaatccc gggctcttta tgcgggagct
    13501 gggggcgggc tgcgcggaat tgcgctaatt atcaactcgc aaacggttct tgacggtgct
    13561 cacgggggga gaggaataca gagccaaggc ctgtttgacg cagttaccgg acgggcgggc
    13621 gtagcctctg agctgccggc ccaatcagca cgcccggcag gcaaccgata aatcttgtta
    13681 gtagccgcgc gccggcagag cactgataaa acccgaagcg ctagcgaggc ccgggcatct
    13741 gagcatcgga gcccgaagcg caaccgcggc cacaacatcg gcgaaccaaa tgaaaatcgg
    13801 tatcgacgct cgaaccatct tctctcccca gccgcgcggc acaggtcgca acctcttcga
    13861 cgcttatcgc ttgatacctg cgttgcgccc cgactggacg tttcacctgt accatcagcg
    13921 gccggcggcg gcctgcccgc tggtgtgcgg cgcggctgaa gcagatcgac ctgaagaatc
    13981 tgcctggccg gcaaatgttg cgttaaggcg catagatatt ccgggcgacc ggctgaatct
    14041 atggtttcag ttgcgtttac cggttgctgc ctggcgcgac cggcttgatt tgctgcacct
    14101 tcctgccaat gcggccccgg cgtggtgtcc ggttcctacg gtggtcacgg tccacgactt
    14161 ggttccactc aagattgcgg acgaactgcc gccccccgcg cgcgagcgct ttcggcgcgg
    14221 ggtcttacga gcggctcgga cggcacgaca catcatcacg ccctcggcca cgacgcgcga
    14281 cgaactgtgc ggagagtttg gtgtgtgcgc cgatcgagtg acagtcattc cctgggcggc
    14341 ggaccgtctc attgccggca tgagcgatgc gacgtcgtcg agcgctttcc atgccgttcg
    14401 ccgtcgctac gagcttcacc gcccgtggat gctcaacttc tccggcccga ctcgccgcaa
    14461 gaatgcagcc gggttactcg ctgggttcgc ccaactgccg gccgagattc gtgccgtctg
    14521 cgacctcgtc ctggtggggt gcgctacgtc tgagtttcag gccgaactgg ccgcaatggc
    14581 gcagacgctc gggatttccg agcattgccg ctttctggct tttgtgccgc acgcggacct
    14641 gccgtatttg ctcggcgggg cgcgagctct gctcatgccg agtttggccg aggggtttgg
    14701 gctgccgatt ctggatgctt ttgcctgcgg agtgcccgtg ctgacctcgg cgtgcagcag
    14761 catgcccgag gtggccggcg acaccgcgct gtactgcgat ccggtctcgc cggccagtat
    14821 tgcggcgggg atgctgcaaa ttctgcagcg gcaggtggcc acggcgctgg tggcgcgcgg
    14881 gcggcagcgg gtgcaacaat tcacatggga ggcgacggcg acggcgatgt gccaagttta
    14941 tgagcgctgc ctgctcgaaa ccgggcggct gcaggcgcca tacccgcttc cggcgggccg
    15001 ggatgagcgc gggcatgcca cgcaggccgc cggcgcgggc gccgc
//
LOCUS       DAOSYS010000061        14999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3353_length_14999_cov_4.965003, whole genome shotgun sequence.
ACCESSION   DAOSYS010000061 DAOSYS010000000
VERSION     DAOSYS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14999
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_14999_cov_4.965003"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..1181
                     /locus_tag="PLU99_05475"
     CDS             312..1181
                     /locus_tag="PLU99_05475"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOO16542.1"
                     /translation="MAADPLKILSLPKLTNGRMVIAFSGWMDGGNVSTGSVEWLVRTL
                     AATKVAEIDPEGFFIYNFPGSMEVSALFRPHTSYENGQIIAYEPPENEFYCSEAERVI
                     FFTGKEPNLNWERFADCIFRFAAAAGVTTLYFVGSVGGTVPHTREPRLRCTVSDARLK
                     PLLEPFAVRFASYDGPASFSTHLLVRAAARGLAMVNLVAEIPAYIQGTNPKSIEAVIR
                     RLAGILKLEVDVRELRDLGTVWEERLNDALERREDVSTQIRKLEENYDSEVFDTEMGE
                     LKEWLEEQGIRVD"
     gene            complement(1217..3079)
                     /locus_tag="PLU99_05480"
     CDS             complement(1217..3079)
                     /locus_tag="PLU99_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOO16543.1"
                     /translation="MSDFLIQIGDWVSLDAVGDALREREGMGELRISQFRFPWGSAVV
                     QETRGLGYDPASANDCVCFAVGRPRIIGVTHEVEGPQGFTRRWAGWVGKADFDRHYRS
                     LTGLFLLVSCGPERVELVTDRLGFYAAYMGRDRNGRICAIGTLPNFVARAADRLDDLD
                     LVSVGEFLVRYTPTFPHTTYRGVTELAPGTIHTFDVSGTPVTHTCRSLWKPEEPPRYP
                     PLKELRKQLTCALRDAGQELTRGAERVAVTLSGGVDSRTVLACVPPERRAAALTFLDR
                     ENHEYRVAQRAAAGFGVPHLALLRDREFYARLPEREAALLGPIRTAMHAHGMVLAGAA
                     VQFDLVLSGLFADALLKCYFAPETVRATLAARLGVPAWLRTRLCAQRPAWDDPTRPAS
                     QWAVRPQIQRQMQQRAAIRMNEMTAIRPETADEWINFYPASRVSAGSYSMGNMRLFAS
                     DELFFYRDILEVACSCRPLPKMMERLTVPVFAELCGPQARIETTKTGLPADLGWLQTR
                     LRLRWNRLLRRDVPRTNGFNDAPWFADHSWVDLRMLQRCSAAWAALRRRAARAAGVDV
                     LAAVLQIDPRRLLMGFNENLSCEFQVAAVQLALTLGETAAVPEPVLAESMQTST"
     gene            3450..4772
                     /gene="mgtE"
                     /locus_tag="PLU99_05485"
     CDS             3450..4772
                     /gene="mgtE"
                     /locus_tag="PLU99_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOO16544.1"
                     /translation="MPNQISEIDLEALRATWPALSDEERAEAFSLLGEDDAHTFFASL
                     TPADQAGILKHLPNGRAAIWMRMLAPDDAADVLQESDAEQRQRLEAMLPEPTRREVQA
                     LLAYAEDDAGGLMSPRYVRLRPDVTIEVAIRYLRQQIRKNPETLYYVYVLDAEQRLLG
                     VISFRELFAAPGDALVADVMRRDPITVHEATDQEEVARIIETNDLLALPVVDDEGRVK
                     GIVTVDDIVDVIQEEATEDIQKIGGTEALRAPYLDVSFPAMIKKRGGWLSVLFFGELL
                     TTVVMGYFEQEIAQAVVLALFVPLIISSGGNSGSQATTLIIRSMALAELRLRDWWRVI
                     RRELGSGLTLGTWLGLIGFLRVVLWHNAGWANFGEHHVLLGLTVWLSLIGVVSLGTLA
                     GSMLPFLMRLLGFDPAVSSAPFVATLVDVTGLIIYFSIAAVVLRGTLL"
     gene            4855..5697
                     /locus_tag="PLU99_05490"
     CDS             4855..5697
                     /locus_tag="PLU99_05490"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOO16545.1"
                     /translation="MTHDTTDTPVAERLAGTNAGIVERHDLNETLSIVRVRPDSGRIP
                     DFKPGQFVTLGLPKPLTPALAARLAGRPGVVPMVRRAYSVASSPRQTDALELFVVLIE
                     TGKLTPLLWRRDFPNRVFLEDVAKGEFTLDSVPDGQNLVMISTGTGIAPFVSMLRTYR
                     GQNRWRQLVIINGVRRVSDLGYRAELEGVSREDPTVRYVPIVSRPDAAGGWDRLCGRV
                     QVMLDDALCQEHAGLCLDPRSCHVFLCGNPAMIDEMELLLQKRGFRTHTAQTPGNIHF
                     ERYW"
     gene            complement(5780..8182)
                     /locus_tag="PLU99_05495"
     CDS             complement(5780..8182)
                     /locus_tag="PLU99_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16546.1"
                     /translation="MFARSQYRRRFCVLFSVLVIGGLAAQALADNNYLIVTASDYAGS
                     APLNQFIAAKTAMGFSVSTYTAPPGTSRETIKAYIQSLWGTPAAPKFLLIVGDTDGST
                     ATTNTIPHWTGGGSRQATTDLPYACMGGPSDWYPDMCHGRFSVRTVSQLQAVVNKSLF
                     VETGSYPDLDYVKRAAFLATDDSSAQAPQNHDWVISTYLDPAEFTSTRIYASLGGGTA
                     DISAAVNAGCLFVVYLGHSGSGGWSSPGFSQSNVNALLNEGLYGLVIGWSCNTAHYSY
                     DECFGETWQRAPNKGAAAYLSASDYVWWGSVDSWESSRRMERYFFRAIFEKNIWQVGP
                     AWQSALFTILNDPDFGPSHDHTRNIFEEMVLLGDPALMLPRGNGFRLRVDPPAFDICT
                     PPATSLQYSINVELMGEFTEEVTLSTTGLPAGVTASFSANGLPPPFETTLTIDGLTAD
                     MAGTYSAGLTGASQSQQRSVALGLSIHAEPPAPVTLLEPTGDAMDVPLKPQFTWEPTA
                     DAAEYALQVATDAGFVNLVIDTTLTEARFTPAQPFGTLASYYWRVQAANACGVGEWTP
                     ARRFRTVNMIGPVSYDMLNGQTGSYTYFDDTYDGDGSVTTPLAPLSNGLGDLTDGVIA
                     TQHWNQTPVPYVGWNSVDPVITFHFAHPVWITQVVVHVDDSAGGGGVYPPDDITITFG
                     GETHVYQVTEPPGSTPFAITLPVDLIGDTMVMTIADHSSSSYIMVSEVEFHGNPIPGD
                     TNCDGAVNAFDIDPFVLALTDPVAYGVAQPVCNILTADCNDDGEVNAFDIDPFVMLLT
                     RP"
     gene            8495..8719
                     /locus_tag="PLU99_05500"
     CDS             8495..8719
                     /locus_tag="PLU99_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOO16547.1"
                     /translation="MQIRCKCRDLSFVGLLAYARRHGITDIEELIKATGCCTGCGSCR
                     PLLEELLRSGKLRVGDQLIDFPLPDDETAG"
     gene            complement(8905..9204)
                     /locus_tag="PLU99_05505"
     CDS             complement(8905..9204)
                     /locus_tag="PLU99_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO16548.1"
                     /translation="MQADEDREYEQEELDWEAREALEDPEGPQECDLLAQGDPDEPDT
                     VECPNCGEDILEDADQCPHCHQWVIQGGQTDAQSHGFVFILIAALILVVLAYWLQ"
     gene            complement(9204..9764)
                     /locus_tag="PLU99_05510"
     CDS             complement(9204..9764)
                     /locus_tag="PLU99_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOO16549.1"
                     /translation="MAVMCLNRTQAQLVVVDVQEKMLAHIHAREDVLAQIVRMMRAAH
                     ELELPLTISEQYVRGLGPTVPQVLSAAGEPARFTKLEKMAFSLMRDEPLREHIVGLRR
                     PQVVLVGIESHVCVQQTAFDLLRLGLEPCVLADAVGSRRTSDRDVALDRMRAAGIAVS
                     TVESAIFEMLELSGTDLFKRLLPIVR"
     gene            9963..11666
                     /locus_tag="PLU99_05515"
     CDS             9963..11666
                     /locus_tag="PLU99_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOO16550.1"
                     /translation="MMYTLHSYVLRELLKTFALTLVALTAVLTMGGGLYNVLRYEGAS
                     AGDVLGFLPMLIPIMVALAMPVAAIFATTMVYGRLAADNEFTACRAAGINVHKLFVGA
                     LLVSVFVAVFTLLFGDLVVPDVTRRLNQYVRANLRDLALQRMETKGYVRYQRGGSTYL
                     LTAENMRVPSERALQEQNLPTGEGFSYLLMDSPFFVMLDKDERLQRYACAKHGLCQFD
                     GRQSPLQVTLFVNGGRDFDVHASSAYLESQQIGPISVPLEFPRRPGMLDLATLMRWRD
                     RPWEIEQLQPKIRSFQLNLARSQFADDCTALLEQGQPIELDNERGDVCRIYAARAEVE
                     DKGLLLYDARVEQPAADGAAPLYYQAPLVRLRARTAASEELFLQSAGRLDTALVVRVE
                     LDRTAEQPIVEHNPRASDYAHGREKPPFALSEDYRVPAHILTRLQSYTEEVVFDPQAD
                     LPFSPELTVARQRLLADAAAQQRKMSANIQSRLGIACSALVTVLMAAALGAMFRGSQA
                     LAAFGLACIPFGAAALAIVMARSLGERPDLEFLSPLITWGGLGLIGVADVVILRLGVR
                     R"
     gene            11704..12792
                     /locus_tag="PLU99_05520"
     CDS             11704..12792
                     /locus_tag="PLU99_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOO16551.1"
                     /translation="MLTIDRYIIGSFLRSYFILLAVGLGVYIVSDLLVNLDGFIENRE
                     LSFSEVLNAIVDYYWHNLPLYFSQLAGPVTAFAGAFTLAMMLRNNEMTALVSAGMPLQ
                     RLAAPVFACAALLIAVWMLNREFVLPAFAADIARDRRDVIGIRTRNIDFARDDNKAIL
                     RAGAINLNENRLERVIIIEPKDQGSHIIQADAATYDPQRKTWLLEGGRRVVEVPAAEL
                     IGAEQFGIRREPVSEYPFTLTPQELILRQSAEWAGMLSLREMNELVHSRNLPNLAAIV
                     MNRHIWLTQPVLQLLLLALTVPFFLRREPCSVLVAGGQALLLSGAFYATGFFAHSMIA
                     AQHAALVAWIPILIFGPIAALLLSNIRT"
     gene            12971..13651
                     /locus_tag="PLU99_05525"
     CDS             12971..13651
                     /locus_tag="PLU99_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO16552.1"
                     /translation="MAHIVIIEDEPDLAMGLRDNLEFEKHTVAHALTGQEGIALVSRK
                     PPDLILLDIMLPDIDGFEVCRRLRAAGFRMPILILSARSQEIDKVRGLELGADDYITK
                     PFSLKELLARIQAALRRAATPAKPVLQFRVGQRMVDLVAQKITTDKEELPLGYYESEI
                     LRMLYERVGMAVPRADLLEQIWGAGVYPADRTVDNHIVSLRRKLEPDPKNPRHILTAH
                     AVGYKLVL"
     gene            13805..14086
                     /locus_tag="PLU99_05530"
     CDS             13805..14086
                     /locus_tag="PLU99_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16553.1"
                     /translation="MRIVALLASLMLVAGAAGEQFWIEYDASCGLFPEECGWNRLAME
                     GGAERSLDDGLLTLDSLAGLGVIDCYGIGRPISLAPGESFVMQWRLRVN"
     gene            complement(14784..>14999)
                     /locus_tag="PLU99_05535"
     CDS             complement(14784..>14999)
                     /locus_tag="PLU99_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16554.1"
                     /translation="YADGRSERRPLVGDIHLSYIGRSGLFNAIVEPQRQSALIGAIVL
                     EDLDLIPDCVNQTLIPRDPDRIISEVE"
BASE COUNT         2646 a         4682 c         4917 g         2754 t
ORIGIN      
        1 cgtccacggc ctgcacctgg tccgcgagaa ttgggagcaa ctgccaaacc tgtcacgaca
       61 caactagagc acgtcttaca ccgtgctcgg ggtcgcgccc gttgtcccac ataaataagc
      121 gccgttaacg aaggcatgcc cgcgcttaga gcttgggcat ggcaccccat ggcactgcat
      181 ggcaccccat ggcacccact cgcgcccgtc ggtaggggcc ggaccggtta accggtgaat
      241 tctcgctgtt accgctaacc gtggattacc gccgccgtgc ccgctattct ctctaaacag
      301 cggagaacca gatggcggcc gacccactca aaatccttag tttgcccaag ctcaccaacg
      361 gtcgcatggt gatcgccttc tccggctgga tggacggcgg aaacgtgtct accggcagtg
      421 ttgaatggct tgtaagaacg ctcgcggcca caaaggtggc tgaaatcgac cccgaaggct
      481 ttttcatcta caacttcccc ggttcgatgg aggtttcggc cctgtttcgg ccgcacacca
      541 gctacgagaa cggccagata attgcatacg agccgccgga gaacgaattc tactgcagcg
      601 aagcggaacg ggtgatcttc ttcacgggca aggagccgaa cctgaattgg gagcggtttg
      661 ccgactgcat cttccgcttc gcggcagcgg cgggcgtaac gacgctgtac tttgtcggca
      721 gtgtcggcgg aaccgtgccg cacacgcgcg agccgcggct gcgttgtacg gtttcggacg
      781 cgcgacttaa gccgttgctc gaaccgttcg cggtgcgttt tgcgagctat gacgggccgg
      841 cgagcttctc gacgcacctg ctggttcgcg ctgcggcccg gggactggcg atggtgaacc
      901 tcgtggccga gattccggcg tacattcaag gcaccaatcc caagagcatc gaagcggtca
      961 tccggcggct ggcggggatt ctcaagctgg aagtggacgt tcgcgagctg cgcgacctcg
     1021 gcactgtctg ggaagagcgg ctcaacgacg cactggagcg gcgggaggac gtctccacgc
     1081 aaatccgcaa gctcgaagag aactatgaca gcgaagtctt cgacacggaa atgggtgaat
     1141 tgaaggagtg gctcgaggag cagggaatcc gggtcgactg agctgtttgt ctcccgcagt
     1201 ttcagcttcc gcagcttcag gtggacgttt gcatggattc ggccaggacc ggctctggta
     1261 ctgcggccgt ttcgccaaga gtcagcgcca gttggaccgc ggccacctgg aattcgcaac
     1321 tcaagttctc attaaagccc atcagcaagc ggcgcgggtc gatctgcaac accgctgcca
     1381 ggacatcaac acccgccgcg cgggcagcac gacggcgcag ggcagcccat gcggcggaac
     1441 accgctgcaa catccgcagg tcgacccagg agtgatccgc aaaccagggc gcgtcgttga
     1501 agccgtttgt gcgcggcacg tcacgcctca gcagcctgtt ccagcgcagc cgcaaccgag
     1561 tctggagcca gcccaggtcg gccggcaagc cggtcttggt cgtttcgatg cgtgcctgcg
     1621 ggccgcacag ttcggcaaaa accgggacag tcagccgctc catcatcttt ggcaacggcc
     1681 ggcaggaaca agccacttcg agtatgtccc gatagaagaa caactcgtcg gacgcaaaca
     1741 gccgcatgtt gcccatggag tagctgcctg ccgaaacgcg gctggcgggg taaaagttga
     1801 tccattcatc ggccgtttcg gggcggatgg cggtcatctc gttcatgcgt attgcggcgc
     1861 gttgctgcat ttgccgctga atttgtggcc gcacggccca ctggctggcc ggtcgagtcg
     1921 ggtcatccca ggcgggacgt tgagcgcaca ggcgcgttcg gagccacgcc ggcacgccca
     1981 ggcgtgcggc cagcgttgcc cggacagtct ccggcgcgaa atagcatttc agcagggcgt
     2041 cggcgaagag cccgctgagc acgaggtcga attgcaccgc ggccccggcc agaaccatgc
     2101 cgtgcgcgtg cattgcggtt cggatcggcc ccagcagggc cgcttcgcgt tcggggaggc
     2161 gagcatagaa ctcgcgatct cgcagcaagg ccaggtgcgg aacgccgaag cccgccgcgg
     2221 cgcgttgcgc gacccggtac tcgtggtttt cgcgatccag aaacgtgagc gccgcggcgc
     2281 gcctttcagg cggcacacag gccagtacgg tccgcgaatc tacgccgccg ctgagcgtca
     2341 cggcgacccg ctccgcgccg cgcgtaagtt cctggccggc atcgcggagt gcgcaagtca
     2401 gttgtttgcg gagttccttg agaggcggat agcgcggcgg ctcctccggc ttccacaaac
     2461 tgcggcaagt atgtgtgact ggggtgcccg agacgtcaaa cgtgtgaatc gtgccgggcg
     2521 ccagttcagt tacgccgcga tacgttgtgt gcgggaaggt tggcgtataa cggactagga
     2581 attcgcctac ggaaaccaga tccaggtcgt ccaggcggtc cgccgcgcgc gccacgaagt
     2641 tgggcagagt gccaatcgcg catatgcgcc cgtttcggtc gcgccccatg tatgccgcat
     2701 aaaagccgag ccggtccgtc acgagttcga cccgctcggg cccgcaggag acgagcagga
     2761 acagtccggt aagactgcga tagtgccgat cgaaatcagc ctttccaacc caaccggccc
     2821 agcgacgcgt aaagccctgc ggcccttcca cctcgtgcgt cacgccgatg atgcgcggcc
     2881 gcccgacggc aaagcagacg cagtcattcg cgctggccgg gtcatagccg aggccgcgtg
     2941 tctcctgcac tacggcgctg ccccagggaa accggaactg cgagattcgc agttcgccca
     3001 tcccctcccg ctcgcggagc gcgtcaccga cagcatccaa gctcacccaa tcgccgattt
     3061 ggatcaggaa atcactcata agaacactgt gaacattccc gctggcccgc gccgcctccg
     3121 gattgccaat tctacggcca cgccggagcc gaaagtcaac ttccgcttcg cgcgtctgac
     3181 cgcgtagccc ccgatgtttg tcgtcatcca ctatttacca tccgccatct ccgccgcagc
     3241 ccgcgatcca ccgtcaacgc gtcacggcca ctcgaggcgc gaaactcgta acttgaaact
     3301 gaaacacgcc gcagccagaa tacgccccgc catggagagt accggcgcca cgctcgaccc
     3361 ggacggccct gctagatggc ccagccggca caaggtgtag acgcccggcg cgctggctga
     3421 tcccgccgcc gggcgggagg ccagcagcta tgcccaacca aatcagcgaa atcgacctcg
     3481 aagccctgcg agccacctgg ccggcgctgt cggacgagga acgcgccgag gctttcagcc
     3541 tcctggggga agacgatgcc cacaccttct tcgcctcgct cacgcccgct gatcaggcgg
     3601 gcattctcaa gcatctgccc aacggcagag ccgcgatctg gatgcgcatg ctcgcgccgg
     3661 acgatgcggc cgacgtactc caggagtccg acgccgagca gcggcagcgc ctcgaagcta
     3721 tgctccccga gccaacccgc cgggaggtgc aggccctact ggcctacgcc gaggacgacg
     3781 ccggcggttt gatgagcccg cgctacgtac ggctccgccc ggacgtcact atcgaggtgg
     3841 ccatccgtta tttgcggcaa cagatccgca agaacccgga gacgctgtac tacgtttacg
     3901 tgctggatgc cgagcagcgc ctgctgggcg tgatctcgtt ccgcgagctg ttcgccgcgc
     3961 ccggcgacgc cttagtggcc gacgtgatgc gccgcgatcc gatcaccgta cacgaagcca
     4021 ctgaccagga agaggtcgcc cgcataatcg aaaccaacga cttgctcgct ctgcccgtcg
     4081 tcgatgacga gggacgcgtc aaaggtatcg tcaccgttga tgacatcgtc gacgtcatcc
     4141 aggaggaggc taccgaggac atccagaaga tcggcggtac tgaggccctg cgtgccccct
     4201 acctggacgt ctcattcccc gccatgatta agaaacgcgg cgggtggctc tccgtactct
     4261 tcttcggcga actgctaacc actgtcgtca tgggctactt cgagcaggaa atcgcccagg
     4321 ccgtcgtgct ggccctcttc gtgccgctga tcatcagcag cggcggcaac tccggctcgc
     4381 aggccacgac ccttattatc cgttccatgg ccctggctga gttgcgcctg cgcgactggt
     4441 ggcgcgtcat ccgccgcgaa ctgggttcgg gcctgacgct gggcacctgg ctggggttga
     4501 tcggcttctt gcgtgtcgtg ctttggcaca acgccggctg ggccaacttc ggcgagcatc
     4561 acgtgttact gggtctgacg gtctggctca gcctgatcgg cgtcgtaagc cttggcacgc
     4621 tggccggcag catgctgccg ttcctgatgc gcctgctagg tttcgacccc gcggtcagtt
     4681 cagccccgtt cgtggccacg ctggtagatg taaccggctt gataatctac ttcagcatcg
     4741 cggccgtcgt gctgcgcggt acgctattgt gacgccaaat gcggcttatg cgacgaagaa
     4801 tggtcgccac tccgccggca agaacgccgc cccgggttca ctagaatcag ggatatgaca
     4861 catgacacga cggacacccc cgttgccgaa cgcctggccg gaaccaatgc cggcatcgtc
     4921 gagcggcatg acctgaatga gacgctcagc atcgtgcgcg tccggccgga tagcggccgc
     4981 attcccgact tcaagccggg ccaattcgtc acgcttggtc tgcccaagcc gctgacgccg
     5041 gccctggcgg cgcggctggc cggtcgcccc ggcgttgtcc cgatggtgcg ccgggcgtat
     5101 tcagtcgcct cctcgccgcg gcagaccgat gcgctggagc tgttcgtcgt gctgattgaa
     5161 accggcaagc tcacgccgtt gctttggcgg cgcgacttcc ccaatcgcgt ttttctggag
     5221 gacgtcgcca agggcgagtt cactctggat tccgtgcccg acggccagaa cctggtcatg
     5281 atctcgaccg gcaccggcat cgcaccattt gtttccatgt tgcgcaccta ccgtggccaa
     5341 aaccgttggc ggcaactggt catcatcaac ggcgtacggc gcgtctccga cctcggctat
     5401 cgcgcggagc tcgagggcgt cagccgggaa gaccccaccg tgcgctacgt gcccatcgtc
     5461 tcgcgccccg acgctgccgg cggctgggac cgcctctgtg gccgcgtgca agtcatgctc
     5521 gacgacgcct tgtgccagga gcacgccggg ttgtgcctgg acccgcggag ctgccacgtg
     5581 ttcctgtgcg gcaacccggc catgatcgac gagatggagc tgctcctgca gaagcgcggc
     5641 ttccgcacgc acacggcaca gactccgggc aacattcact tcgagcgtta ttggtagccg
     5701 acaccggcgg aaggcgctga tagcggtcct gggccggatc tatcgggcca gtcctcggcc
     5761 aggggggccg gcgctactgt cagggcctgg tcaggagcat tacgaacggg tcgatgtcga
     5821 acgcgttcac ctcgccatcg tcgttgcagt cggcggtcag aatgttgcac accggttggg
     5881 cgaccccgta ggcaaccggg tccgtcagcg ccagtacgaa cgggtcgatg tcgaatgcgt
     5941 tcaccgcgcc gtcacagttg gtgtcgccgg gaatcggatt gccgtgaaac tcgacctcgc
     6001 tcaccatgat gtagctgctc gaactgtgat cggcgatcgt catcaccatg gtgtcgccga
     6061 tcaagtccac gggcagcgtg atcgcgaagg gggtgctacc gggcggttca gttacttgat
     6121 atacgtgcgt ttcgccgccg aaagtgatcg ttatatcgtc cggcggatac acgccgcccc
     6181 cgccggctga atcgtccaca tgcaccacca cttgcgtgat ccacaccggg tgcgcgaagt
     6241 ggaacgtgat caccgggtcg accgagttcc agccgacgta cggcacgggg gtctggttcc
     6301 agtgctgcgt ggcgattacg ccgtcggtca ggtcgcccag gccattgctc aggggagcca
     6361 gcggcgttgt aaccgaaccg tcgccgtcat acgtatcgtc gaaataggta tacgagccgg
     6421 tctgcccatt gagcatgtcg tatgagaccg ggccgatcat gttcacggtc ctgaagcgcc
     6481 gcgctggagt ccattcccca acaccgcatg cgtttgcggc ctgaacgcgc cagtagtagc
     6541 tggccagcgt tccaaacggc tgggccggcg tgaagcgcgc ttccgtcaga gtcgtgtcaa
     6601 tgaccaggtt gacgaacccg gcgtcggtcg ccacttgcag ggcgtactcg gccgcgtcgg
     6661 ccgtcggctc ccaggtgaat tgcggcttca agggcacgtc catggcgtcc ccggtcggct
     6721 cgaggagcgt aaccggcgcc gggggctcgg cgtggatgct caagcctagc gcaaccgagc
     6781 gctgctggga ctgggacgcg ccggtcaggc ccgcactgta cgtgccggcc atatccgcgg
     6841 tcagcccatc gatcgttaac gtggtttcga acgggggcgg caggccgtta gcgctgaagc
     6901 ttgcggtgac gccggccggc aggccggtcg tgctcagcgt gacctcttcg gtaaactcgc
     6961 ccatcagttc gacattgatc gagtattgca ggctggtggc cggcggggtg cagatgtcaa
     7021 acgcgggcgg atcaacccga aggcgaaagc cgttgccgcg cggcagcatc agggccggat
     7081 cgcccagcag caccatttct tcaaagatgt tccgggtgtg gtcgtgcgac gggccaaaat
     7141 cggggtcgtt caggatggtg aacaaggccg actgccaggc cggccctact tgccatatgt
     7201 tcttttcaaa gatcgctcta aagaagtagc gctccatgcg gcgcgaggat tcccaggaat
     7261 ccacgctgcc ccaccagacg taatcagagg cggacaggta ggccgccgcg cccttgttcg
     7321 gggcgcgctg ccacgtctcg ccgaagcact catcatagct gtagtgggcg gtgttgcagg
     7381 accagcctat caccaggccg tacaaaccct cgttgagaag tgcattgacg ttcgactgac
     7441 tgaaaccggg cgatgaccag ccgccggaac cgctgtggcc aaggtaaacg acaaaaaggc
     7501 agccggcatt cacggcggcg ctgatgtcgg cggttccgcc gccgaggctg gcgtaaatgc
     7561 gggtgctggt gaactcggct gggtctaaat aggtggagat gacccaatcg tggttctgtg
     7621 gggcctgggc gctcgagtca tccgtggcca ggaaggcggc gcgcttgacg tagtcgaggt
     7681 ccggataact tccggtttcg acaaacaggc tcttgttgac tacggcctga agctggctca
     7741 cggtgcggac ggaaaagcgc ccgtggcaca tgtcggggta ccagtccgac gggccgccca
     7801 tgcaggcgta cggcagatcg gtcgtggctt ggcggctgcc accgccggtc cagtgcggga
     7861 tcgtgttggt cgtcgccgtc gagccgtcgg tgtcgccgac aatcagcagg aattttggag
     7921 cggccggcgt gccccagagg ctttgaatgt aggccttgat cgtctcgcgc gatgtaccgg
     7981 gcggcgcggt gtaggttgag accgaaaagc ccatggccgt tttggcggcg atgaactggt
     8041 tcagcggcgc cgatccggca tagtcggagg cggtgacgat caggtagttg ttgtcggcca
     8101 aggcctgcgc cgccaggccg ccgatcacga gaacggaaaa caacacacaa aagcggcggc
     8161 gatattgaga tcgcgcgaac atgccttcct ctcctggccg ctaagggccg tatagcatta
     8221 caagctgaac tcagcgctgc ggcgactggt taggtcggcg cggtgcagac cttcacgatg
     8281 tccaggcgac tcagcgcggc tgtacggtca gcatagcaca atcgcggggc ggactgcaat
     8341 gcggagtttg gcggagggcg ggggggcgac gggagggatt agttgttagc tattagttat
     8401 tagtactggc ggcttgggcc ggttgggcga ttggttatta gtgctgcttg ggggacaggc
     8461 cccggctccc cttcgcgccg gtatactgag gcgcatgcag atacgctgca aatgccgcga
     8521 tttgagcttc gtcgggctgc tggcgtacgc gcggcggcat gggattaccg atattgaaga
     8581 gctgataaaa gcgaccggct gctgcaccgg ttgcggaagc tgccggccgc tgctggagga
     8641 attactacgc agcggcaagc tgcgcgtggg ggatcagctt atcgactttc cgttgccgga
     8701 cgacgagacg gcggggtagc agcgaggttt cacttgggcg agacggcggg ttgactcatt
     8761 tggccgagaa cgcgggtagg cgcgagattt cgtttggcga gacggcgggg tggcggcggg
     8821 gttcccttgg tgctgttgtg ggggcacggc ttggcgcttg gggatggcgc acaacccgtc
     8881 gatcggcgtc gcccacgggg gcggctattg aagccagtag gccagcacga ccaggatgag
     8941 cgccgcgatc aggatgaaga cgaagccgtg cgactgtgcg tcggtctggc cgccttgaat
     9001 gacccactgg tggcagtgcg ggcactggtc ggcatcctcc aggatgtcct cgccgcaatt
     9061 gggacactcc accgtgtccg gctcgtccgg gtcgccctgg gcgagcaggt cgcattcctg
     9121 cggcccttcc gggtcttcga gcgcttcgcg cgcctcccag tccagctctt cctgttcgta
     9181 ttcgcggtct tcgtctgctt gcatcagcgc acgatcggca acagccgctt aaagaggtcc
     9241 gttcctgaaa gttccagcat ctcgaagatg gccgattcca cggttgaaac ggcgatgcct
     9301 gcggcgcgca tgcggtccag ggccacgtcg cggtccgacg ttcgccgcga gccgacggcg
     9361 tccgccagca cgcacggctc caggcccagt cgcagcaggt caaaggcggt ttgctgcacg
     9421 cagacgtgcg attcgatgcc gaccagcacc acctgcgggc ggcgcagccc gacgatgtgc
     9481 tcgcgaagcg gctcgtcgcg catcaggctg aacgccattt tctccagctt ggtgaaacgg
     9541 gccggctcgc cggccgccga cagcacctgc ggcacggtcg gcccgagccc gcgcacgtac
     9601 tgctcggaaa tcgtcagcgg cagctccaac tcatgcgccg cccgcatcat ccgcacgatc
     9661 tgcgccagca catcctcgcg ggcgtgaatg tgcgccagca tcttctcctg gacgtccacg
     9721 acgacgagtt gcgcttgggt ccgattgaga cacatcacag ccatgtttgc cctcttcgac
     9781 cgacagcgcg taatctgaca gaaaccgggc catgtggcaa ccgcggcgtg ccaaacgcgg
     9841 gccgctgaat gacccatgct tgcccgccgg ctccggccgc cctacattga aacgttctcg
     9901 ttcagcggcc ggcgcccgca gcgctggccg cggccgtgtc ttcccacgag gccagaacgg
     9961 cgatgatgta cacgctgcat tcttacgtgc tgcgcgagct gctgaagacc tttgcgctca
    10021 cgctggtggc cctgacggcc gtgctcacca tgggcggcgg gctgtacaac gtgctgcgct
    10081 atgaaggggc cagcgccggc gacgtactgg gctttttgcc catgctgatc cccatcatgg
    10141 tggcgctggc catgccggtg gcggccatct tcgccaccac catggtttac gggcggctcg
    10201 cggccgataa cgagttcaca gcctgccggg cggcgggaat caacgtacac aagctattcg
    10261 tgggggcgct gttggtgtcg gtgtttgtgg cggtgttcac gctgctgttc ggcgacctgg
    10321 tggtccccga cgtaacacgg cgcctgaacc agtacgtgcg ggccaacctg cgcgacctcg
    10381 ccttgcagcg gatggagacg aagggttacg tgcgctatca gcgcggcggc agcacctacc
    10441 ttttgacggc cgagaacatg cgcgtgccca gcgaacgggc cctgcaagag cagaacctgc
    10501 cgaccggcga gggcttcagt tacctgctga tggattcgcc gttcttcgtg atgctcgaca
    10561 aggacgagcg gctgcagcgc tatgcttgcg ccaaacacgg gctgtgccag ttcgacgggc
    10621 gccagtcgcc gctgcaggtc acgctgttcg tgaacggcgg acgcgatttc gatgtgcacg
    10681 ccagcagtgc ttacctcgaa agccagcaaa tcgggccgat ttccgtgccg ctcgagtttc
    10741 cacgccgccc gggcatgttg gatctggcca cgctcatgcg ctggcgcgac cggccgtggg
    10801 agatcgaaca gttgcagccc aaaatccgca gtttccagct caacctggcg cggtcgcagt
    10861 tcgccgacga ctgcactgcc ctgctggagc agggtcagcc gattgagttg gacaacgagc
    10921 gcggcgatgt ttgccgtatt tatgccgcca gggccgaggt ggaagacaaa ggcttgctgc
    10981 tgtacgacgc gcgcgtcgaa caaccggcgg cggatggggc ggcgccgctg tactaccagg
    11041 cgcccctggt gcgtttgcgc gcccggacgg ccgccagcga agagctgttc ctgcaatcgg
    11101 ccggccgcct cgatacggcc ctagtggtcc gcgttgagct ggaccgcacc gccgagcagc
    11161 cgatcgtcga gcacaacccg cgggccagcg actacgccca cgggcgtgag aaaccgccgt
    11221 tcgcgctgtc ggaagactac cgcgttccgg cgcacattct gacccggctg cagagttaca
    11281 cggaggaagt ggttttcgat ccacaggccg acctgccttt cagcccggag ctgactgtgg
    11341 cccgccagcg gctactggcg gatgccgccg cgcagcagcg gaagatgtcg gcgaacatcc
    11401 agtcgcgcct gggcatagcg tgcagtgcgt tggtgaccgt gctgatggcc gcggccttgg
    11461 gggcgatgtt tcgcgggtcg caagcgctgg ccgcgttcgg gctggcctgc atcccgttcg
    11521 gagcggcggc cttggcgatc gtgatggcgc gttcgctggg cgagcggccg gacttggaat
    11581 ttctctcgcc gctgatcacg tggggcgggc tgggactgat cggcgtggcg gacgtggtga
    11641 ttctgcggct gggcgtgcgg cgatgaccga atcttgcagt gccatgacgg agtctgcagt
    11701 gcgatgttga cgatcgaccg ttacatcatt ggcagtttcc tgcgctccta cttcattctg
    11761 ctggcggtcg ggctgggcgt gtacatcgtc agcgatctgc tggtcaacct ggacggcttc
    11821 atcgagaacc gcgaactgag cttcagcgaa gttcttaacg cgatcgtcga ctattactgg
    11881 cacaatctgc cgctgtactt ctcgcagctt gccgggccgg tgacggcctt cgccggggcc
    11941 ttcacgctgg ccatgatgct gcgcaacaac gaaatgacgg cgttagtgtc ggccggcatg
    12001 ccactgcagc gcctggcggc gccggtgttc gcgtgtgcgg cgctgttgat cgcggtgtgg
    12061 atgctcaacc gcgagtttgt gttgcccgcc ttcgccgccg atattgcccg cgaccggcgg
    12121 gacgtcatcg gcatccgtac gcgcaacatc gacttcgccc gggacgataa taaagccatc
    12181 ctgcgggccg gcgccatcaa cctgaatgag aaccgcctgg aacgcgtcat catcatcgaa
    12241 cccaaagacc agggcagcca catcatccag gccgacgcgg ccacgtacga tccgcagcgc
    12301 aagacctggc tgctggaggg cgggcggcgg gtggtcgagg tgcccgcggc cgaacttatt
    12361 ggtgcggagc agttcggcat tcggcgcgag ccggtgagcg aatatccgtt caccttgacg
    12421 ccgcaagagt tgatcctgcg gcagtcggcc gaatgggccg gcatgctcag cctgcgcgaa
    12481 atgaacgagt tggtacacag ccggaatttg cccaatctgg cggcgatcgt gatgaaccgg
    12541 cacatctggc tgacgcagcc ggtgttgcag ttgctgctat tggcgttgac ggtgccgttt
    12601 ttcctgcggc gtgagccgtg cagcgtgctg gtggcgggcg ggcaggcgtt gctgctgagc
    12661 ggggcgtttt acgcgacggg attcttcgcg cacagcatga ttgccgcgca gcacgcggcg
    12721 ctggtagcat ggatcccgat cctgattttc ggcccgatcg cagcgttgct gctgtcgaat
    12781 atcaggacgt aggttttgag cagggatcaa gggatctagg gatcgaggga tcaagaaact
    12841 tgttcccggc ccgatgcgcg agcgcccatc cgagcccgaa gcgcgagcga gggcaaacgg
    12901 gccggctcga agcgccggcg agggcaacga gggctaaacc ggcgataccc cgctttgtgg
    12961 agaacctgac atggctcaca tcgtcatcat cgaggacgag ccggatctgg cgatggggtt
    13021 gcgcgacaat ctcgaattcg aaaagcacac cgtcgcgcac gcacttaccg gccaggaggg
    13081 catcgcgctc gtcagccgca aaccgcccga cctgattctg ctcgacatca tgctgccgga
    13141 catcgacggc ttcgaagtct gccggcggct gcgggcggcg ggtttccgca tgccgatcct
    13201 gatactcagc gcccgcagcc aggagatcga caaggtgcgc ggcctggagc tgggcgcgga
    13261 tgattacatc accaagccgt tcagcctgaa ggagctgctg gcgcgcattc aggcggcgtt
    13321 gcgccgtgcg gccacgccgg cgaagccggt cctgcaattt cgggtggggc agcggatggt
    13381 agacctggtg gcgcagaaga tcacgacgga taaagaggag ctgccgctgg gctattacga
    13441 gtcggagatt ctgcggatgc tgtacgagcg ggtcggcatg gcggtgccgc gggccgacct
    13501 gctggagcag atctggggcg ccggcgtgta cccggccgac cggacggtgg acaaccacat
    13561 agtgagcctg cgacggaagc tggaacccga cccaaagaac ccgcggcata tcctcacggc
    13621 gcacgcggtg gggtacaagc tggtgctgta gcgcgggtga agcagtggtc accggctagc
    13681 gccgtcgcgg cgcgaaacgg ggaccgggct gcgggcccgg cctgcccgag cataaattcg
    13741 tgagggcgcg gtgcagatca acgcgggtta tcggtctggc gtcttcgcaa ggaaggggcg
    13801 aactatgcgt attgtcgctc tattggcatc gctgatgctc gtcgcgggcg ccgccggcga
    13861 gcagttctgg atcgaatacg atgcaagctg cggcttgttc ccggaggagt gcggctggaa
    13921 ccgcctcgca atggagggtg gtgccgagcg ctcgttggac gatggtctgc tgacgctaga
    13981 tagcttggcg ggcctgggcg taatcgactg ctatggcatc ggccgcccga tcagcctcgc
    14041 gccgggtgag tcgttcgtta tgcaatggcg actgcgcgtc aactaagttc acggggccgc
    14101 ggcgcggccc atcaagccct caatctccgt ggcatttgat ggctacggtc atgccatcct
    14161 ggcgttctcg gagagttcac tgtacaattg gtttgaccac tctggggtgg attttgaacc
    14221 atatgtcttc catgactact cgttaacaac gagcgacctg gtgacataca cggtaacaat
    14281 cgacggcacc ttcgcgttca gtgcgcaatt ctatacaccc tgtttcggct cctgggccga
    14341 ctggggcgac tcaccagcga tttcttgaca tattcgctgt cagtcgatga cagcgtcgtt
    14401 tatgtgggag acctggcctc atcgcccggc gcccccggcg ttgcctgggg cgatgcgact
    14461 gacggggcaa gcagtctgtc agtgtggcaa tacgtacgct tcggcgttgt tcccgaaccg
    14521 tccgctgctt tgttaatcat ttccgttggc ctggcaggag tcatcctgcg ggcgcggtca
    14581 gcggggacag tgttgcaatc gtgaaaggtg gctgatcgag aatcggggac gcgccgacgg
    14641 cgctagttgt taggtagcac ggatgccttg ggtcgcgagg aggcagacgt tgacaatgcg
    14701 gcgcgcggcg ctgcgtcggc cacggcgggc cgacgctacc ttcccacctc cccactttgc
    14761 caccttccca ggcgcgtacc gcctcactca acttccgaaa tgatccggtc cgggtcgcgc
    14821 ggtatcagcg tctggttcac gcagtccggg atgagatcca ggtcctccag gacgatcgcg
    14881 ccgattaggg ccgactggcg ctgcggctcg acgatcgcgt taaacagccc gctccgtccg
    14941 atgtacgaaa ggtgaatatc gcccaccagc gggcggcgct cgctccgccc gtcggcgta
//
LOCUS       DAOSYS010000062        14994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3355_length_14994_cov_4.940692, whole genome shotgun sequence.
ACCESSION   DAOSYS010000062 DAOSYS010000000
VERSION     DAOSYS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3355_length_14994_cov_4.940692"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PLU99_05540"
     CDS             <1..533
                     /locus_tag="PLU99_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HOO16555.1"
                     /translation="RTAEHDDPMLTCRIGRPRRIATRVVLDAALRTPERSQLVRTARQ
                     TPTWLFCSPRAARSRIRRLRSAGCQVHVVPPDPSGAGVSLPPVLDTLGAHGMTNVLVE
                     GGGQVLGRFFEQKLGDELQIYLAPMLVGGAAAPGPLAGSGPARIADCLRLEPKPVIKP
                     LGEGWLLQARLRAVVP"
     gene            complement(638..1438)
                     /locus_tag="PLU99_05545"
     CDS             complement(638..1438)
                     /locus_tag="PLU99_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16556.1"
                     /translation="MSKAVKEAPREAAADVMGDALLTAAGVCKFYEMGSTQLRVLDDC
                     NLRVSAGEFVAIMGKSGSGKSTLLHVLGALDVPQRGQVMFAGQPVFAPPNARRLTSGM
                     TDVLSGAERRRIELRRRAFGFVFQFYYLLPELNVLENVLITRMVGTKTLGWWRGGSAA
                     ARADALRILTRVGMHERLKHRPNELSGGERQRVALARALVHKPRVLFADEPTGNLDAE
                     AGRKIMDLLKELHHEGQTIVMVTHDSGVARYADRVLLLADGRLQPASV"
     gene            complement(1431..3041)
                     /locus_tag="PLU99_05550"
     CDS             complement(1431..3041)
                     /locus_tag="PLU99_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOO16557.1"
                     /translation="MVLIVLSVMGGWLDMVKTHARGLLGDVVIDNHDHSGFPLYQEFI
                     DDITTWKDAEGRPLIEKATPVLYSWGLFKLQDYLGTARVVGIRLEEVFEVNAFKQSLF
                     YNKYYPGTTTLKEQQQPLLGADLDAGPLRITTESGDVLPPMIRPTLPSPHREALEKTL
                     QANGGRLEVENEVPTFTSELLMQAGLPPIPGYYELALRESPGGEAIGSVDLPRQDGPA
                     FPGIIFGLDLVAARQPDGKYNRRYPRGEVASLTLWATSARGQVDPIPIKQPFRYTDDS
                     RTGIYEIDSQHVYCDFDLLQKLLQMQETPRVNADGEIIGMAPARCAQIQIKLARPLDE
                     MSLQALTTRLEERYYSLADDPRFDLDLTDRQLIARIDAMTWQQTQAHLIGPVEKERIL
                     VTILFGIISLVAVALILCILYMIVLQKTRDIGIVKAIGGSSGGVAFVFVIYGAAVGIA
                     GAVLGTILGCLVVWNINEIQDFLIRLNPSWRVWDLSVYSFDRIPSEVNPWDAVIVVVF
                     AILASTLGSLGAAWRAGRMQPVEAIRHE"
     gene            3340..4110
                     /locus_tag="PLU99_05555"
     CDS             3340..4110
                     /locus_tag="PLU99_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOO16558.1"
                     /translation="MFGLKSRRRNRLRAQAIPAQWSAAIQSNVPYFHLLTPAERVELF
                     GHVQVLVHEKPFEGCGGLVVTDEVRVTIAAQAAILLLGRQTDYYPKLRTILVYPAKYV
                     AEVARPLPGGAVLAGPEARLGEAWYRGAVVLSWDDVLRGAADPGDADNVVFHEFAHQL
                     DSESGANEGAPDLPTREARARWARVFEREFRRLINDLQHARPTLLRPYAATNPAEFFA
                     VVTEYFFELPLALRSRHAELYDEMAAYFQQDPTRRFLR"
     gene            complement(4300..5112)
                     /locus_tag="PLU99_05560"
     CDS             complement(4300..5112)
                     /locus_tag="PLU99_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOO16559.1"
                     /translation="MSEEQRENTASGGGGGGGSARHGGGGHGGHSGGHEEAHEGAPEW
                     LISFADNVVLQMGFFVILLALTLTSVKSGAGGTDSGEAAGGPTAEQLDWAIAVREAFN
                     NPVDLHSTDPRDLLLVRRLRARRGLADADSLGQKGADHDVKSIRPSDYFGWGGTVPFA
                     QESSVLDEAGKAALAEMLEHLRGYGSVLEIRGHASAAEAFGKADRGAGLSYERARAVA
                     EALAAGGIDWKQLRVLACADSERIVPRAADADGHARNQRVEVVVTEQAPLVE"
     gene            complement(5140..5919)
                     /locus_tag="PLU99_05565"
     CDS             complement(5140..5919)
                     /locus_tag="PLU99_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOO16560.1"
                     /translation="MDIASLIGSLIGIAACLVVGYRASEGNWAMFYSEKGAIMVFGGT
                     ISVVLMAMPMEKLKCVPGYVRRFMFQRGMAAADVIRLMGQLSEKARRDGILSLESELP
                     KINDPFLATGIKMAIDGAAPDAVEATLRMEVLAMQDRHKAGKKFFDLIKLYAPGYGLV
                     ATLIGQIGMFGHLGGEVSVMGHMLAVAVVATMYGCILANAIAGPIGDKLSLRSSEEII
                     NREMMLQGILSIQSGDNPRVTVDKMMAFVPVPQRSRIRQAA"
     gene            6187..7803
                     /locus_tag="PLU99_05570"
     CDS             6187..7803
                     /locus_tag="PLU99_05570"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOO16561.1"
                     /translation="MPTNLAEETLPSDGTRTEPTVPAKRGFEFDRLAIPLALALLAVL
                     TVPRLPPGICSGDSGDLQVAAVTLGMAHPPGYAGLASVGHLLTLIPGLEPAYAVTLGC
                     LASGLIALAFIAGLQIRLGVNPWLAAAVALVLASNARVWEALVVPEVYAPNLALHAAA
                     AWFLLTYLQTGRLGRLAVTALLLGFALANRPSTVLSIPGFILAVLVFWWQNRPARSRW
                     AAATTLSVAGLVLPCVYTLGYYYVRDNSETPVNMIENRRLDYGDLPAATDGPAAKIER
                     VLWLATAREFKGLLGGTWPWMSGRGRWLVERLGLSGDLSMIAVTCATVIGGALAWRRC
                     RSTTLALMGVALGAVAFIFAYQVYDTAADVLPIILAAGVFIGLCASALLPGRTAITRE
                     ALSMSLFIVAGFWTASQADQPVTGSSVLDASRSVPAAEVQNLPYRAALLSDWMNWPAV
                     RYAQHRLGRNDLLIAIPKHRSALAVRLHDGGSGRPVFSTMQFSPPPGYQITPWGELWR
                     FDAVEPPGSEADGTVPPERNDAVSEGADEQ"
     gene            complement(7702..9264)
                     /locus_tag="PLU99_05575"
     CDS             complement(7702..9264)
                     /locus_tag="PLU99_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16562.1"
                     /translation="MTAPEEHSSSAPQQPSSGPQTQQPAIDPAQRFRRRLVFAMLCLA
                     GLIARLAALALAPEHAFLSDHVDYMAWSHYAYEHGPTSVYDAPAGNLINVNLPPWLFG
                     RRVTMPFPSYSRCNYPPLAAYAFWGQGFAWSLLEPQPATLKPHPEIAAEVGYTGGPVT
                     SRIANTVPARLANSFLALLTDFLWAFGVAKLVQRLRPQWPAAGTWAFGIALLAPPVFI
                     NSAFWNQTDSWLACGMVWTIYLLTTNRYAAAGVVYGATALIKAQAVLLAPVLVYAFVG
                     LALSRSQARLRRALPAFVGAAVVTVVVLAAPFSVRDAGGGEMGGARWIYRSYIEPIQS
                     DFPYTTLKAFNVWWLDQAAHGGTREALDAHAQVFGFSKDAVGRTLLIVALLALALLCA
                     RRAGWSPAAWQFFAALALLAVFLLPTRAHERYLYYALPFIIAGALLFRRWLPMLIAVL
                     LVATFEMTWYLWYSPAFARPWDPPANPRAAAWSVLLALLLVSAFAYGVITLWRNRTVG
                     LAAGRLDRIEAP"
     gene            complement(9462..12281)
                     /locus_tag="PLU99_05580"
     CDS             complement(9462..12281)
                     /locus_tag="PLU99_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16563.1"
                     /translation="MNALRTNGSVASITAALTLMLAGSAVAQENKYLIVSAPDYVGSA
                     ALTEFIAFRTSRGYEVSVYNVPAGTSREDIKAYIESLWGTPDAPDYLLIVGDTSGAAS
                     TSNTIPHWVGAGTRQAPTDLPYACMDGPGDWYPDMYYGRFSVTSPGMLQQVVDKIIRV
                     ESGDFPDPSYLRRAAMLATDDPTAEAAALHDSIISTYLEPAGFSATRIYAGMGGSTAD
                     IAAAVNAGVLFTVYFGHSGTGGWSSPGFWQANVNALTNDGLYGLTMGWSCNSARFDQD
                     ECYGETWLRAANKGSAAYLSASAGIPSGSATAWDCSWRMERYFFQSLFEDDIHEVGRA
                     WQQALWKMLDDPDYGPGHSETRNIFDEMVLLGDPALRLPIRSLALSLPDGCPEYISPQ
                     APTPIRVRIENVCETYEPGTGLLHYRFAGGAYLTTPLTPLGDGLYQALLPAPRCSAVP
                     EFYFSATGHLGGPAKFPRNAPATVLTATVATVTTLLDDDFETARGWSVWIEPTMESGF
                     WQRTIPASQPLPGAPAADFDGSGRCYVTDNRVGNYDVDGGPTRITSAAFDLSGMTDVR
                     VCYARWIYCDDYSGPDQDFLEVEFSSDNGATWVPVEQVSDLGGWVKREIAVQDYVPLT
                     AQFRVRFSISDTPNNSVTEAAVDDVLIYDLSCHAPPLPGDLNCDDAVNAFDIDPFILV
                     LTGDPTCAAYYAAFPNCDHQRADCNGDGEVNVFDIDAFVTLLMGASGACCDDATGICA
                     DYVAPADCMTRYALGEQCSDLEPVCGNPGACCDDVTGECLDYIFELLCTGRFAGGVLC
                     ADLAPQCAPCADAELMAPGSWSGNTCGAVNDCAMRPSQDLIVQVEIPTAGSWTFDLCA
                     GTTVWDTYLYVGHACCTSTWYNDDGCPTGAALSTLSIASLPAGVYYATIEAYSSACGP
                     VTLTVSTGTADRTDASGAAELMHEN"
     gene            complement(12492..13412)
                     /locus_tag="PLU99_05585"
     CDS             complement(12492..13412)
                     /locus_tag="PLU99_05585"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOO16564.1"
                     /translation="MTTNSVTKRVRLQRFLADAGVTSRKHAEEYIAAGRVLVNGEVVR
                     ELPAFVDPQHDEVVVDGNPVRAAAPLYYLVNKPKGVVSGPRERAGRRRVSELLPPLRQ
                     KLRPAGQLDEEDSGLLLMTNDGRLAQRLAHPQFGLPKTYWVEVKGRAPDDIAVRLLQG
                     VYLAEGKARAMNVEVGFASNDRSTLTVTVREGRKRQVRRMLARLGLPVRNLKCTQIGP
                     LKLKGLPLGASRALSPGELNALHAELARSADQHKVRRAGGARVKRSQTPSGRQRAPGA
                     AAPAAGRSPARGRPRGGNSGREQPGRRIVS"
     gene            complement(13409..>14994)
                     /locus_tag="PLU99_05590"
     CDS             complement(13409..>14994)
                     /locus_tag="PLU99_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO16565.1"
                     /translation="ADCPTFVREHLINEMAKERESWADLEARKRFSPADLIDSPATAV
                     VEIVGHVPPGAARNTTFDIMVRAIPGTATCSLAGGLLLPTNLHLSDPVGSGEALLSMP
                     IVARAAGPIFVNPFATPEDAEGLRTGYVLSGGQCSEERTFRLLLREPSYPMTRRIALR
                     INERFGQRPPIADATSRGFITLKMPPEYSQDRAHFLRLLPHVYIDGTAAFSEGKLRQL
                     MQYAVQPGYDLEAISLAWEGIGPTAIPQLKPLYTHADARVRFYAARAGLRLKDPDAVP
                     PLSALSRGTDRDLALLSIAELGRCNLPYAAMLLRPLLDVDDAELRIAAYEAALNYSTT
                     FIQTRRFPHLLDPLQLNLALDIVESSGRPLIYVRRTGIPRIAVFGRRLSLTLPCFYAE
                     ADDSVILNALDESSDLTILTNRYNQPWDPLHVSPRVEDLIAALADLPTKNELDQPRGI
                     GLPYSQVVHVLAALSRDQTIAAPVKIEQASMVELFGPAPQIERLETDPSDDAAPAESD
                     APTDGRADSDTGSEPGPTP"
BASE COUNT         2597 a         4867 c         4895 g         2635 t
ORIGIN      
        1 ggcgaacggc cgagcacgac gacccgatgc tcacctgccg catcgggcgg ccgcgccgga
       61 tcgccacgcg tgttgtcctg gatgcggccc tgcgcacgcc cgagcgttcg cagctcgttc
      121 ggacggcccg gcaaacgccc acttggctct tctgcagccc ccgcgctgcg cgcagccgca
      181 tccgcagact gcgatcggcc ggctgccagg tacacgtggt tccgccggat ccaagcggcg
      241 ccggtgtgtc gttgccgccc gtgctggata ctctaggtgc gcatggaatg acgaatgttc
      301 tcgtagaagg cggcggccag gtcctggggc gcttcttcga acagaagctg ggggatgagc
      361 tgcaaatcta cttggccccg atgctggtcg gcggcgccgc ggctcctggt ccgctggcag
      421 gcagcggacc ggcccggata gcggactgcc tgcgcctgga gccgaagccg gtgatcaagc
      481 cgctggggga gggttggttg cttcaggcgc ggctgcgggc cgtagtcccg taacattcga
      541 gcccgaagcg ccagcaccga tgcctgttaa gcgttcagcc tgagcaattg ggggtactct
      601 ggtcaaactc cggctcgaca cgagccggct ctgctaccta gactgacgcg ggctgcaatc
      661 ggccgtcggc cagcaagagc acccggtccg cgtagcgcgc tacgcccgaa tcgtgtgtga
      721 ccatgacaat ggtctgccct tcgtggtgca gttccttcag cagatccatg attttgcggc
      781 cggcttcggc gtcgaggttg cccgttggct catccgcgaa gaggacgcgc ggcttgtgca
      841 ccagagcccg cgccagcgcg acccgctgcc gctcgccgcc cgaaagctcg tttggccggt
      901 gcttgaggcg ctcgtgcatg ccgacgcgcg tgaggattct cagtgcgtcc gcccgtgcgg
      961 cggctgaacc gccgcgccac cagcccagcg tcttcgtgcc gaccatgcgc gtaatgagta
     1021 cgttttccag cacgttcaac tcgggcagca ggtagtagaa ctggaaaacg aacccgaacg
     1081 cccggcggcg cagctcgatc cgccgccgct ccgcgccgga gagcacgtcc gtcatgccgg
     1141 atgtcagccg ccgcgcgttc ggcggcgcga acaccggctg gccggcaaac atcacttgtc
     1201 cacgctgcgg cacgtccagc gcccccaaca catgcagcaa cgtgctcttg ccgctgccgg
     1261 acttgcccat gatggccacg aattcgccgg ccgaaacccg cagattgcaa tcgtccagca
     1321 cgcgcagttg cgtgctgccc atctcgtaaa acttgcatac tccggcggcg gtcagcaacg
     1381 catcgcccat cacatcagcc gcggcctcgc gcggcgcctc cttgacggct ttactcatgg
     1441 cggatcgcct ccaccggctg cattcggccc gcgcgccagg ctgctccgag cgaacccagg
     1501 gtcgacgcca gaatggcgaa caccacgacg atcaccgcat cccacgggtt aacctcgctc
     1561 ggaatacggt cgaacgaata caccgacaag tcccacaccc gccaactcgg attgaggcgg
     1621 atcaggaaat cctgaatctc attgatgttc cagacgacca ggcagccgag gatcgtaccc
     1681 aaaacggctc cggcaattcc cacggccgcc ccgtagatga cgaagacgaa ggccacgccg
     1741 ccggacgagc cgccgatggc tttgacgatc ccgatgtcgc gcgtcttctg cagcacgatc
     1801 atgtagagaa tgcacagaat gagtgcgacc gcgaccagcg agatgatgcc aaagagaatg
     1861 gtaaccagga tgcgctcttt ctccacgggg ccgatcagat gcgcctgcgt ctgctgccag
     1921 gtcatcgcgt caatgcgggc gatgagctgg cggtcggtca gatccaggtc gaaacgtgga
     1981 tcgtcggcga gcgagtaata ccgttcctcc aggcgcgtcg tgagggcctg cagactcatc
     2041 tcgtccagcg gtctggccag cttgatttgg atctgggcac aacgggccgg ggccatgccg
     2101 atgatctcgc cgtcggcatt gacgcgtggg gtttcctgca tttgtagcag cttttgcagc
     2161 aggtcgaagt cacagtagac gtgctgcgaa tcaatctcgt aaatgccggt gcgcgagtcg
     2221 tcggtgtagc ggaacggctg cttgatgggt atgggatcga cctgcccacg ggccgaggtc
     2281 gcccacagcg tcagcgaagc cacttcgccg cgcgggtagc ggcgattgta tttcccgtcc
     2341 ggctggcgtg cggcgaccag gtccaggccg aagatgatgc ccggaaaagc cgggccatcc
     2401 tgccgcggca aatcgactga gccgattgcc tctccgcctg ggctctcccg cagcgccaac
     2461 tcatagtaac ccgggattgg cggcagcccg gcctgcatca atagctcgct ggtgaaggtg
     2521 gggacttcat tttctacttc cagcctgcct ccattggctt gcagcgtttt ttcgagcgct
     2581 tcgcggtgag gcgacggcag ggtgggccta atcatgggcg gcagaacgtc gccgctttcg
     2641 gtggtaatcc gcagcggacc ggcgtccagg tcagctccga gcagcggctg ctgctgctct
     2701 tttaaggtgg tcgtgccggg atagtacttg ttgtaaaaaa ggctttgctt gaaggcgttg
     2761 acctcgaaga cctcctcgag ccgaatgccg accacacggg ccgttcccaa atagtcctgc
     2821 agtttgaaca gcccccacga gtacaagacc ggggtggcct tttcgatcag cggccggccc
     2881 tcggcgtctt tccaagtcgt gatgtcgtcg atgaattcct ggtacagggg gaagccggaa
     2941 tggtcgtgat tgtcgatgac gacgtcgccc aacaggccgc gggcgtgcgt cttaaccatg
     3001 tccagccagc cacccatcac cgacaggacg atcagcacca ttgccacgca cagcgtgacg
     3061 gccgcgatcg cgaagtaggc gatacgccgt ttgcgcaggt aacgtagagt aaggaataac
     3121 ttgtacagat cggtctcttt cgctgtgtgg gcaggccacc gctacccttg cgatggcagc
     3181 acgctcgcct cgtgtgccgc ccgccgccta gtcccatata atgtggcgcg gcgtcggggc
     3241 tggcaaggcg acgcggcgca atatcggtct tgctgtaatc caagacccgc gcgtggcggt
     3301 ttcgttgggc cttcgggaca aaatcgagct tacgcaggca tgttcgggct caaaagcaga
     3361 cgccgaaatc gcctccgggc gcaggctatc ccggcccaat ggtcggccgc cattcaatcc
     3421 aacgtgccct actttcatct gctcacgccc gccgagcgtg tggagctgtt tggtcacgtg
     3481 caggtactgg tgcacgagaa gccgttcgag ggctgcggcg gcttggtcgt gaccgacgag
     3541 gtgcgcgtca ccatcgccgc ccaggccgcg atcctgctgc tgggccggca gacggattac
     3601 tatccgaagc tgcgcacgat actcgtttac ccggcgaagt acgtggcgga agtggcgcgc
     3661 ccgctgcccg gcggggcggt cctggccgga cccgaggcgc gattaggcga ggcctggtac
     3721 cgcggcgccg tggtgctgtc ctgggacgac gtgctgcgcg gcgccgcgga cccaggcgat
     3781 gcggacaatg tcgttttcca cgaattcgct caccaactcg acagtgagtc cggcgccaac
     3841 gagggcgccc cagatttgcc cacgcgcgag gcgcgcgcca ggtgggcccg tgttttcgaa
     3901 cgcgagttcc gccggctgat taacgacttg cagcatgccc gccccacgtt gctgcgcccc
     3961 tacgccgcca ctaacccggc cgagttcttc gcggtcgtaa ccgagtactt cttcgagctg
     4021 ccgctggctc tgcgcagccg gcatgccgag ctgtatgacg aaatggcggc ctacttccaa
     4081 caggacccaa cccgccgctt cctccgttga tcaggtgttg accgtgcatc ggcgctgtgc
     4141 gcagtggcag agccggctcg tgtcgagccg gaatttgacc aagtgccccc caatcgtcca
     4201 ggtcgcttgc cttggtgttc gacgtcggcc acggggggcc gacgctacgg gctgcgtgcg
     4261 ctcgctggcg cttcgggctc ggatttgggc gtcggttggc tattccacca gcggcgcctg
     4321 ctccgtcacc accacctcca cacgctggtt gcgggcgtgg ccgtccgcgt ccgccgcgcg
     4381 cggcacgatc cgctcgctgt cggcacaggc cagcacgcgc agttgcttcc agtcgatccc
     4441 gcccgctgcc aaagcttccg cgaccgcccg cgcccgctcg tacgatagcc ccgccccgcg
     4501 atccgccttg ccaaacgcct cggccgcgct ggcgtgcccg cgaatttcga gaacgctccc
     4561 gtagccccgc aaatgctcga gcatttcggc cagcgctgcc ttgccggctt cgtccagcac
     4621 gctggactcc tgcgcgaagg gcaccgttcc gccccagccg aagtagtcgc tgggccggat
     4681 cgacttcacg tcgtgatcgg cccccttttg ccccagactg tcggcgtcgg ccaacccgcg
     4741 ccgggctcgc aggcgccgga ccaacagaag gtcgcgcgga tcagtggagt gcaaatcgac
     4801 cgggttgttg aacgcctcgc gcaccgcgat cgcccaatcg agttgctccg cggtcggtcc
     4861 gccggccgcc tcgccggaat cggtgccgcc cgccccgctc ttcaccgaag ttaaggtcaa
     4921 cgccagcaga atgacaaaga accccatttg gagcacgacg ttgtccgcaa aggagatcaa
     4981 ccattccggc gcgccttcat gggcctcctc gtgtccccca gagtgcccgc cgtgacctcc
     5041 gccgccgtgc cgcgcgctcc cgcccccgcc cccgccgccg ctcgccgtgt tttcgcgctg
     5101 ctcctcagac atgttcagtc acgctccggc cgcctcgcgt tatgcggcct gacgaattcg
     5161 ggaccgttgc ggcacgggga caaaggccat catcttgtcg accgtcacgc gcgggttgtc
     5221 gccggactga atggagagaa tcccctgcag catcatttcg cggttgatga tctcctccga
     5281 agaccgcagc gaaagcttgt cgccgatcgg tccggcgatg gcgttggcca gaatgcagcc
     5341 gtacatcgtg gccacgaccg cgaccgccag catgtgcccc atgaccgaca cctccccgcc
     5401 caggtgcccg aacattccga tctggccgat cagggtggcc accagcccgt agccgggggc
     5461 gtagagcttg atcaagtcga agaatttctt gccggccttg tggcggtcct gcatggcgag
     5521 cacttccatg cgcagggtgg cctcgacggc atccggcgcc gcgccgtcga tggccatttt
     5581 tattcccgtc gccaggaagg ggtcgttaat cttggggagt tcggattcca gcgaaagaat
     5641 tccgtcgcgg cgggccttct cgctgagttg ccccatcaga cggatcacgt cggccgccgc
     5701 catgccccgc tgaaacatga accgccgcac gtaccccgga acgcacttaa gcttctccat
     5761 cggcatggcc atcagcacca cgctgatggt gccgccgaac accatgatcg cccccttctc
     5821 ggagtagaac atggcccagt tcccttccga ggcccggtag ccgaccacca ggcaggcggc
     5881 gatgccgatc agtgaaccaa tgagactggc aatgtccacg ttgcgcctcc ggcggcgcgc
     5941 gcgacggttg ggcgagcttt cgacccgtcc gcggccgagt tgggcactat gcgaataatc
     6001 ggctcgcgcc gcgggccact ccacaaggaa gctgggccgc gcagcgtccg aaacggcttg
     6061 tggtggtgac cgcgagaagg cagcgtttct gagacctgcg gcgcgactct aagatgctgg
     6121 gagcgctcct ggggaaaaag ggcgggcagg ggccagctca gttataatcc ggcccagccg
     6181 cctgctgtgc cgaccaacct tgcggaggag acgttgccgt ccgacggcac aagaactgag
     6241 ccaactgttc ccgcaaaacg cgggtttgag ttcgaccgcc tggctatacc gctggcgctg
     6301 gcgttgctcg ccgtcctgac cgtcccgcgc ctgccgcccg gaatctgttc cggcgattcg
     6361 ggcgacctgc aggtcgcggc cgtcacgctc ggcatggccc atccccccgg ctacgccggc
     6421 ctcgcctccg tggggcacct cctgacgctt attcccggcc tggagccggc ctatgcggtc
     6481 acgctcggtt gcctcgccag cggcctgatc gccctggcgt ttatcgccgg cttgcagatc
     6541 aggctgggcg tcaacccgtg gctggcggcg gcggtggccc tcgtcctggc cagcaatgcg
     6601 cgcgtctggg aagctttggt cgtgcccgag gtttacgccc cgaacctggc attgcacgcc
     6661 gccgccgcct ggttcctgct cacgtacctg caaaccgggc ggctggggcg tttggctgtg
     6721 accgcgcttc ttctgggttt tgccctggcc aaccggccat cgacggtgct gtctatcccc
     6781 ggcttcatac tggccgtgct cgtcttctgg tggcagaacc ggccggcccg cagcagatgg
     6841 gcagccgcaa ctacgctgag cgtcgccggg ctggtgcttc cgtgcgtcta cacgcttggt
     6901 tattactacg tccgcgacaa ttcggaaacg ccggtgaaca tgatcgagaa ccggcgcctt
     6961 gattatggag acttgcccgc ggccacagac ggtcccgcgg ccaagatcga gcgtgtgctc
     7021 tggctggcga cggcgcgcga gttcaagggg ctcctgggcg gaacgtggcc gtggatgagc
     7081 ggacgcgggc gctggctggt ggagcgtctg gggctctccg gcgatttaag catgattgcg
     7141 gtgacctgtg ccacagtcat aggcggcgcg cttgcctggc gccgttgccg gtccacgacg
     7201 ttggcgctga tgggcgtcgc gctgggtgcg gtggcgttca ttttcgccta tcaggtctac
     7261 gacaccgccg ccgatgtgct gccgatcatc ctcgccgcgg gcgtcttcat cggtctgtgc
     7321 gcttcggcgt tgctgccggg taggaccgct atcacccggg aggcgctgag catgtcgctt
     7381 ttcattgtcg ccgggttttg gacggccagc caggcggatc agcccgttac gggttcaagt
     7441 gtgctcgacg cctcgcgttc tgtgccggcc gcggaggtgc aaaacttgcc gtaccgcgcc
     7501 gcgttgcttt cggactggat gaactggccg gcggtgcgct atgcgcaaca ccggctcggg
     7561 cgaaacgacc tgctgatcgc gataccaaag catcggtcag cgctggcggt tcgacttcac
     7621 gacggcggtt cgggccgccc cgttttcagc acgatgcagt tttcgcctcc gccggggtat
     7681 caaatcactc cttggggtga actatggcgc ttcgatgcgg tcgagccgcc cggcagcgag
     7741 gccgacggta cggttccgcc agagcgtaat gacgccgtaa gcgaaggcgc tgacgagcaa
     7801 tagcgcgagc agcaccgacc aggccgcggc tcgcggattg gccggcggat cccaggggcg
     7861 ggcgaaggcg ggtgagtacc agagatacca ggtcatctcg aacgtcgcga ccagcagcac
     7921 tgcaatcaac attggcagcc aacgcctgaa gagcagtgcg ccggcgatga tgaacggcaa
     7981 agcatagtac aagtagcgtt cgtgggcgcg cgtgggcagg aggaacaccg ccaacagcgc
     8041 cagagccgcg aagaactgcc aggcggccgg tgaccacccg gcgcgccgcg cgcagagcag
     8101 cgccagcgcg agcagcgcca ctatcagcag agttcggccc acggcatcct tgctgaagcc
     8161 gaacacctgg gcgtgcgcat cgagagcctc gcgcgtgccg ccatgggcgg cctggtcgag
     8221 ccaccaaacg ttgaacgctt tgagcgtcgt atagggaaag tcggactgta tgggctcgat
     8281 atacgagcgg taaatccagc gcgccccgcc catctcgccg ccgcctgcat ccctgaccga
     8341 gaagggggcc gccaggacga cgacggtgac gacggcggcg cctacgaaag ccggcagcgc
     8401 ccgccgcagg cgcgcctgcg accgcgaaag cgccagcccg acgaaagcgt ataccagaac
     8461 cggcgccagc agcaccgcct gcgccttgat cagggcggtc gcaccgtaga ccacgccggc
     8521 ggctgcgtag cggttcgttg tgagcagata gattgtccag accattccgc acgccagcca
     8581 tgagtcagtc tggttccaga acgcggaatt gatgaacacg ggcggcgcca gtagggcgat
     8641 accgaaggcc caggtgcccg cggcaggcca ctgcgggcga aggcgttgca cgagcttggc
     8701 gacgccgaac gcccacaaga aatcggtcag cagcgctaga aaagagtttg ccaggcgcgc
     8761 cggtacggtg ttagcgatgc gggatgtaac cgggccaccg gtgtagccga cctcagccgc
     8821 gatttccggg tgcggcttga gcgtcgcggg ctgcggttca aggagtgacc atgcaaatcc
     8881 ctgcccccag aaggcataag ccgccagcgg ggggtaattg cagcggctgt agctcgggaa
     8941 gggcatggtg acgcggcggc cgaagagcca cggcggcagg ttgacgttga tcaggttgcc
     9001 ggcgggtgca tcgtagacgc tggtgggccc gtgctcatag gcataatgcg accaggccat
     9061 gtaatcgacg tggtcggaca ggaacgcgtg ttcgggcgcc agcgcgagtg cggccagccg
     9121 cgcgatcaat cccgcgaggc agagcatggc gaacacgagc cggcgccgaa atcgctgcgc
     9181 cgggtcgatg gcgggctgtt gcgtctgcgg accggagctg ggctgctgcg gagccgagct
     9241 actatgctct tcgggggccg tcatgggttc ggatatagct ggctcgcgct cgagtgtcga
     9301 atcttgagga cgagttttca agggcggctg cggccggtct catcataggc cggtggcggc
     9361 cggacgtcga tccgcgccac ttcaggcgag aaacgctggg cgccgggtgg agccgtcacg
     9421 ccatttgtag aaggcgcacg gcgtgccgac ggcgctcaga gttagttctc atgcatcagc
     9481 tcggcggcgc cggaggcatc ggtgcggtca gccgttcctg tactgacggt cagcgtcacg
     9541 ggcccacacg cactgctgta ggcttcgata gtggcgtaat aaacgccggc gggcagcgac
     9601 gctattgaca gggtggataa cgccgcccca gtcgggcaac cgtcatcgtt gtaccaggtg
     9661 ctggtgcagc aggcatggcc gacgtagagg tacgtatccc aaacggtggt gccggcgcag
     9721 aggtcgaaag tccaggagcc ggctgtcggg atctccacct gcacgatcag gtcttgcgat
     9781 ggacgcatgg cgcagtcgtt gacggcgccg caggtgttgc cgctccagct tcccggagcc
     9841 atgagctcag catcggcgca cggcgcgcac tgcggggcca ggtcggcgca aagcactccg
     9901 ccagcgaaac ggccggtaca cagaagctcg aagatgtagt ccaggcattc gccggtaaca
     9961 tcgtcacagc acgcgccggg gttcccgcag accggctcca ggtctgagca ctgctcgccg
    10021 agagcgtagc gcgtcatgca gtccgcgggt gcaacgtaat ccgcgcatat tccggtggcg
    10081 tcgtcgcagc aagcgcctga tgcgcccatc agcagcgtga caaaagcgtc gatatcgaaa
    10141 acgttgactt ctccatcgcc gttgcagtcg gcgcgctggt gatcacagtt tggaaaggcg
    10201 gcatagtagg cagcgcacgt cgggtcaccg gtcaacacca gtatgaatgg atcgatgtcg
    10261 aacgcgttca cggcgtcgtc gcaattcaaa tcgcctggca ggggcggcgc gtggcagctc
    10321 agatcgtaaa tcagaacatc atccacagcg gcctcggtga ctgagttgtt cggcgtatca
    10381 ctgatggaga acctcactct gaactgcgca gtcagcggta cgtagtcttg tacagcgatt
    10441 tctcgcttga cccacccgcc cagatccgaa acctgctcca ccgggaccca ggtcgcgccg
    10501 ttgtcagagg agaattcgac ctccagaaaa tcctggtcgg gtcctgagta atcgtcgcag
    10561 taaatccacc gggcataaca cacgcgcacg tcggtcatgc cgctcaggtc gaacgccgcc
    10621 gaggttattc gcgtgggccc gccgtccacg tcgtaattgc ctacgcgatt gtcggtcacg
    10681 tagcaccggc cggagccgtc aaagtcggcc gcaggcgccc ccggcagcgg ctggctggcc
    10741 ggtatggttc gttgccagaa cccgctctcc atcgtcggtt cgatccagac gctccagcca
    10801 cgtgcggtct cgaaatcgtc gtccaggagc gtagtgacgg tcgcgacggt tgcagtgaga
    10861 acggtcgcgg gtgcgttcct ggggaacttg gccgggccgc ccaggtggcc agtcgcgctg
    10921 aaatagaact ccggaacagc gctgcagcga ggcgcgggca aaagggcctg gtaaagcccg
    10981 tcgcccaacg gcgtcagcgg ggtggtcaag tacgcaccgc cggcgaaacg atagtgcagc
    11041 aggccggttc cgggttcgta cgtttcgcag acattctcga tgcgcacccg gatcggggtg
    11101 ggcgcttggg gggaaatgta ctccgggcac ccgtcgggca gcgacagcgc cagcgaccgt
    11161 atcggcagcc tcagggctgg atcgcccagc agcaccattt catcaaatat gttgcgcgtt
    11221 tcggagtgcc ccgggccgta atccggatca tccagcatct tccacagcgc ctgctgccag
    11281 gcccgcccga cctcgtgaat gtcgtcctca aaaagcgact ggaaaaaata gcgctccatg
    11341 cgccaagaac agtcccaggc cgtggcgctg ccactcggga tgccggccga agccgacagg
    11401 tacgcggccg agcccttgtt ggccgcccgc agccaggttt cgccgtagca ctcgtcttgg
    11461 tcgaagcggg cactgttgca ggaccagccc atggtcagcc catacagccc gtcattggtg
    11521 agcgcgttaa cgttggcctg ccaaaaaccc ggcgatgacc agccgccggt accgctgtgg
    11581 ccgaagtaca ccgtgaacag cacaccggca ttaaccgcgg cggcgatgtc tgccgtactg
    11641 ccgcccatgc ccgcgtagat tcgcgttgca gagaagcccg ccggttcgag ataggtgctg
    11701 atgattgaat cgtgcagagc cgcggcttct gccgtggggt catcagtggc cagcatcgcg
    11761 gcgcggcgca ggtagcttgg gtcaggaaaa tcgccggatt cgacacgaat gatcttgtcc
    11821 acaacttgct gcagcatgcc ggggctggtt accgagaaac gcccgtaata catgtcgggg
    11881 taccagtcgc cggggccgtc catgcaggca tagggcaggt cagttggcgc ctgccgggtg
    11941 cccgcgccga cccagtgcgg aatcgtgttg ctggtcgatg cggcgccgct cgtgtcgccc
    12001 acgatcagca ggtaatccgg ggcgtccggc gttccccaaa ggctctcgat atacgctttg
    12061 atgtcctcgc ggctggtgcc cgcgggtacg ttgtacacgg acacttcgta accgcggctg
    12121 gtgcggaagg cgatgaattc ggtcaaggcc gcactgccga catagtccgg cgccgacacg
    12181 atcaggtact tgttttcctg cgcgacggcg ctgccggcca gcattagcgt cagcgcggcc
    12241 gttatcgagg ctacactacc attggttcgc agagcgttca ttccggcctc ctccacccca
    12301 ccaatcgcgt taaaaagctg gcgtgcttcc aggtgctgag cgcggggcag caccgtaata
    12361 gcgcctcccg tgcctggcac ttcaaaatac cgcgcccgac tctcgaaaag caatggatgt
    12421 ttggagcgag ttatcggttc gggccgcggg gcgcttcggc cgagagctca caggggaggc
    12481 ggtccggcag gttaagaaac gatgcggcgt cccggctgct cgcgtccaga gttgccaccg
    12541 cgcgggcgac cccgcgcagg ggatcggccc gcggcaggcg cggcggctcc gggagcccgc
    12601 tgccggccgc tgggggtctg ggagcgtttg acgcgtgccc cgccggcacg gcgaaccttg
    12661 tgctggtccg ccgagcgtgc caattccgca tgcagcgcat tcaattcgcc gggactcaac
    12721 gcgcgtgagg cgcccagcgg caggcccttc agcttcaatg gcccgatctg cgtgcatttc
    12781 aggttgcgaa ccggcaggcc cagacgcgcc agcatgcggc ggacctggcg cttgcgacct
    12841 tcgcgcaccg tgaccgtcaa cgtcgacctg tcattgctgg cgaacccgac ctcgacgttc
    12901 atagcgcggg ccttgccctc ggccaaatac acgccttgca gcagccgcac ggcgatgtcg
    12961 tccggagcgc gccccttgac ctcaacccag tacgttttcg gcaggccgaa ctgcgggtgg
    13021 gccaagcgct gcgccagccg gccatcgttg gtcatcaaca gcaggccgga atcttcctca
    13081 tccagttggc ccgccggccg cagcttctgc cgcagcggcg gcagtagttc cgatacacgc
    13141 cggcggccgg cccgttcgcg cggcccgctc acaacgcctt tgggcttgtt caccaggtag
    13201 tacaggggcg cggccgctcg aaccgggttt ccatcaacca caacctcatc atgttgtgga
    13261 tcgacaaagg ccggcagctc gcgcactact tcgccgttga ccagcacacg tccggccgcg
    13321 atgtactcct cagcgtgctt gcgtgaggtg acgccggcat cggccaggaa gcgttgcagc
    13381 cggactcgtt ttgtaacgct gttggttgtc atggcgttgg gccgggttcg gagccggtgt
    13441 cgctatcagc gcgcccgtcg gtgggagcat ccgattcggc cggggcggcg tcgtccgacg
    13501 ggtcagtttc gagcctttcg atttgcggcg ccggaccgaa cagctcaacc atactcgcct
    13561 gctcgatttt gaccggcgcg gcgatggtct gatcgcgaga aagcgcggcc agcacgtgca
    13621 cgacttgcga gtaaggcaga ccgatcccgc gcggctggtc caattcgttc ttggtcggca
    13681 agtcggccaa ggccgcaatc aaatcctcga cgcgtggcga cacgtggagc gggtcccacg
    13741 gctgattgta gcggttggtc aggatcgtca gatcactgct ctcgtccagg gcgttcaaga
    13801 tgaccgagtc gtccgcttcg gcgtagaagc agggtaaggt aagcgacaga cgccggccaa
    13861 acacggcgat gcgcggtatt cccgtgcggc gtacgtagat caggggccgg ccgctggatt
    13921 cgacgatgtc cagcgccagg ttcagttgca gcggatcgag caggtgcgga aagcgccggg
    13981 tctgaatgaa agtcgtcgaa tagttcagcg ccgcctcata ggctgcaatg cgcaattcag
    14041 cgtcatccac gtccagcagc ggacgcagca gcatggccgc gtatggaaga ttacagcggc
    14101 ccaactcggc gatcgaaagc agcgccaggt cacgatccgt gcctctcgac aaagcgctca
    14161 gtggcggcac ggcatccggg tccttgagcc gcaacccggc gcgagccgca tagaaccgca
    14221 cgcgcgcatc cgcatgcgta tacaacggct tgagttgcgg gatggccgtt gggccgatgc
    14281 cttcccacgc cagcgaaatg gcctccaggt catatccggg ctggacggcg tactgcatta
    14341 actgccgcag cttgccctcg gagaacgccg ccgtcccgtc aatgtacacg tgcggcagca
    14401 gccgcaggaa atgcgcccgg tcctgggaat actcgggcgg catcttcagc gtgatgaaac
    14461 cgcggctggt cgcatcggct atcggcgggc gttggccgaa gcgttcgttg atccgcaagg
    14521 ctatgcggcg ggtcatcggg taggaaggct cgcgcagcag caggcgaaag gtgcgttctt
    14581 cgctgcactg cccgccgctg aggacgtagc cggtccgcag gccttcggca tcttcggggg
    14641 tggcgaaggg gttgacgaaa atggggccgg cggcgcgggc cacaattggc atgctcagga
    14701 gtgcctcgcc gctaccgacg ggatcggaca agtgcagatt ggtcggtagc agcaaaccgc
    14761 ccgcgagcga gcacgtcgcc gtccccggga tggcgcgcac cataatgtcg aaagtggtgt
    14821 tgcgcgccgc gccggggggc acgtgaccca cgatttccac cactgcggtc gcgggtgaat
    14881 cgatcaggtc ggccggcgag aatctcttgc gcgcctccaa gtccgcccag gattcgcgtt
    14941 ccttggccat ttcgttgatc aggtgctcgc ggacgaaggt tgggcagtca gcgc
//
LOCUS       DAOSYS010000063        14985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3357_length_14985_cov_5.650100, whole genome shotgun sequence.
ACCESSION   DAOSYS010000063 DAOSYS010000000
VERSION     DAOSYS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14985
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_14985_cov_5.650100"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            442..972
                     /gene="pyrR"
                     /locus_tag="PLU99_05595"
     CDS             442..972
                     /gene="pyrR"
                     /locus_tag="PLU99_05595"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOO16566.1"
                     /translation="MVLLLNAQQLQEKLENLARAIAAAAPRDVPVAVVGIRRRGETLA
                     RRLLPLLERAGLKPAHYGALDITLYRDDLTTIGPSAVVRETEIDFDITDTWLVLVDDV
                     LYTGRSVRAALDALTDLGRPKAIRLAVLVDRGLRELPIQADFVGLRTDTQPQQSVDVR
                     LLEVDGVDEVELNGRR"
     gene            959..1924
                     /locus_tag="PLU99_05600"
     CDS             959..1924
                     /locus_tag="PLU99_05600"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOO16567.1"
                     /translation="MAAAKSAERPVWTRKHLLTLAELTPEEILHILDTAEGFKAVSTR
                     SVKKVPALRGRVVVLMFFEESTRTRMSFELAAQRLSADVIEFSEKMSSTKKGETLLDT
                     ARNIEAMGVDIMVLRHGAAGAANHLAQATKCCVVNAGDGQHAHPTQALLDLFTIRERK
                     GRIAGLNVAIVGDITHSRVARSDLRGLQKLGAHVTFVGPPTLVPKSFEKLGARVTHDF
                     DSILPEMDVVNMLRIQKERIASNVFPSIREYVRLFGMNSERLKRCKPDVLIMHPGPVN
                     RGIELAADVADGPHSSILRQVTNGLAVRMAVLFLCQQAAMPDVEA"
     gene            2078..2482
                     /locus_tag="PLU99_05605"
     CDS             2078..2482
                     /locus_tag="PLU99_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOO16568.1"
                     /translation="MPEITLEQAQAVVNAALERARGMQLKMNIAVVDSGANLKAFARM
                     DGAWLGSIDIAIRKARTARFFDMNTGELGKLSQPGQPLFNIEHSNGGLITFPGGVPLK
                     AGAEIIGAIGVSGSTVENDHAVATAGAAAIQT"
     gene            complement(2518..4095)
                     /locus_tag="PLU99_05610"
     CDS             complement(2518..4095)
                     /locus_tag="PLU99_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOO16569.1"
                     /translation="MRQGTEVAVSEPAPPSEIQQHRSWPTGLLCGALALVIYAASVSP
                     GVEWQDPGRHQFRILTGQMEHPCGLALSHPLHFRLARLALAVPFGEPAWRMNMVSAIC
                     GAAAIGLLAALVVRLTNSRPAAFLAAGVLLVAHSYWQMSCTTETYTLAATLMVCEWLA
                     LLQYARTGRPQWLVLVFALNGLHIANHLLGLIPLATYGVLLIERSLRRKLRPGWLLAC
                     AAVWIAGSAPYWTMVLDFYARTHDLRDTLHSTFFGGGAEYGGWAQAAVGLNLSASQLK
                     IAFLSLGYNFPSLTLLIAALGLLLPMQGPQKLFRWVLLAQTVLMTVFVMRYRIADLYT
                     FFVPLDVMVALWFGLSVASIWRRLRSLTARRACAVLLAVNAVLPPLVYVAFPILARQG
                     EWMEGRWLHRPHRDEYNHFFRPWLRGDHSAAEFACEALERTGPGGWLLGESTTAPTTA
                     YWYLVHGGPPGIQIFNGRVCLTARRPDLTDEELSEFVRTGGNVVIIAGVCTEITWSPG
                     FTLDQSDEYWWRISTGP"
     gene            complement(4079..4609)
                     /locus_tag="PLU99_05615"
     CDS             complement(4079..4609)
                     /locus_tag="PLU99_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO16570.1"
                     /translation="MPVTDPPEPLPEAVTTPAMRQRQPQRWIILGLLALFACWFAAMQ
                     WRQRAGAPVAWERDLPQAEKHARESNQLIFLMLQAPGCPIAAALDRDLFSQRDIREKM
                     AQLTCCRLEVQPTDPIAQRFGFKRNPLMVVVKPGDLRAVGIRPLEGKIQYPELDTVLR
                     ESLKNQGKGHSDASGY"
     gene            4820..6358
                     /locus_tag="PLU99_05620"
     CDS             4820..6358
                     /locus_tag="PLU99_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOO16571.1"
                     /translation="MLRQFPAELEAQRRVIEERARQEGLDFFETIFELVDFDQMNQVA
                     AYGGFPQRYPHWRFGMEYERLRKQHTYGLGKIYEMVINNDPCYAYLLTDNELVDQKTV
                     MAHVYAHCDFFKNNIWFGKTNRKMMDGMANHATRIQRHVEREGLETVERFLDVCLSLE
                     NLIDPYSQFMRRQPEPQPRESAIPAERQPDRAVSRFPAKPYMDRYINPPEMLEAERQR
                     DRRERELQRRAFPAAPERDVLAFLLHHAPLEVWQSDILDIVRDEAYYYAPQGQTKIMN
                     EGWATYWHSYMMTRYHLRDHEVISYADHHSGTLATSPGRLNPYKLGVELFRDIEDRWN
                     RGRFGKEYEECESIEKRRAWDTGLRKGREKIFEVRRVHNDVTFIDEFLTPEFVERYKL
                     YHYRQDPSTGRMVAVDRDFDRIKQTMLFMLTNHGQPYIYIVDANYANRGELYLAHKHL
                     GIDLDIKYAAECLKNLHRLWRRPVHLQARIKDDMTLFTCSGEDVKQQKIKDDMPRPVH
                     TNIE"
     gene            complement(6369..6833)
                     /gene="folK"
                     /locus_tag="PLU99_05625"
     CDS             complement(6369..6833)
                     /gene="folK"
                     /locus_tag="PLU99_05625"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOO16572.1"
                     /translation="MPEGGIYIAMGSNLGDRERHIRTALAELEEQGDIRVLRCSSLRE
                     TDPVGGPPGQPRYLNGAAELDTQLPPHALLQRMLAIEARHGRVRNVKNGARTLDLDLL
                     IYRDQRVTEADLTIPHPRMWEREFVMAPLAEIQSPDQLSKMHQSAASPRPQQ"
     gene            complement(6838..8007)
                     /locus_tag="PLU99_05630"
     CDS             complement(6838..8007)
                     /locus_tag="PLU99_05630"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOO16573.1"
                     /translation="MAFSLPQRLASLPPYLFIDIDRKRRAALAAGHDVINFGVGDPDQ
                     PTPGFIIDRLQRAVRDPVHHRYPPDGGLPEFRQEAADFFRRRYGVDLDPEREVLGLVG
                     SKEGLGHLPLAVVEPGQRVLIPDPAYPVYRAATLFAGGLPYAMQLSAENDWLPQFDAI
                     PAEVARAAVLMFLNYPNNPTGAVAPRTFFERAVEFARRHDLVLAHDAAYSEVYLGSER
                     PPSVLEVEGARDIAVEFHSLSKTFNMTGWRLGFAAGRPDVLAALAKVKANLDSGAFGA
                     IQEAGIEAYAGIDRPEIAATRELYRQRAEVLCAGLRSIGFRVDPPKATFYVWAGVPAG
                     YDGMQVANKLLDEAFVVCIPGIGFGEGAADYVRFALTVDTDRIATAIARLRKLHW"
     gene            8333..10207
                     /locus_tag="PLU99_05635"
     CDS             8333..10207
                     /locus_tag="PLU99_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOO16574.1"
                     /translation="MSTTTSSKKAMATTGKRTETLEQAVIRFAGDSGDGMQLVGTQFT
                     TASAVVGNDVSTFPDYPAEIRAPAGTLGGVSGFQINFGSRAVYTPGDTVDALVAMNPA
                     ALKVCLKDLAPGGVLVVNSDEFTPMNLAKARYETNPLETDELDKYRVYKVPITSQTLL
                     AVKETGLNQKAAERCKNFYALGVVYWLFDRPLDVTIRWIDSKFAKVPAVAEANRRALQ
                     AGYNYGETAEIFSVRYRVGKAPLKPGRYRNVTGNQAAALGFVAAAKLAGKPLFYGSYP
                     ITPASEILHELTIYKNFDVRTFQAEDEIAAYAAVLGAAYAGCIAVTGTSGPGLALKQE
                     GIGLGIMTELPTVIVDVQRGGPSTGLPTKTEQSDLWQAMLGRNGDCPAPVLAASSPAD
                     CFWAAMEAVRIAVKYMTPVFLLSDGYLSQGSEPWRIPNLDELAPIKVEHPTDPADFAP
                     YRRDANGSRPWAIPGTPGLQHRVGGLEKLDVAGTVSYDPANHQRMTELRAGKVENIAA
                     EIPPQEVYGDEAGGALLLVSWGGSFGAVRSAVERAREDGQDVSHVHLRWLNPMPRNLG
                     EILSRFRTVVVCEMNMGQLQMLLRARYLVDAYGLHKVRGRPFMVSDIRAKIDEVLGGR
                     "
     gene            10210..11232
                     /locus_tag="PLU99_05640"
     CDS             10210..11232
                     /locus_tag="PLU99_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOO16575.1"
                     /translation="MTTMADHPVLTIKDYKSDQDVRWCPGCGDFSILTAIQKCLAARQ
                     VPKEKVVCVSGIGCSSRFPYYMDTYGIHSIHGRAPAVATGVKLANPELEVWVITGDGD
                     ALSIGGNHFLHAIRRNIGLKIVLFNNRIYALTKGQVSPTSEQGKRTKSTPYGSIENPV
                     HSIAVALGAEITFAARAIYNDPKMLQDILERAANHKGTAFVEVYQDCAVYNHEAFSYL
                     TDRQTRDDNILYLEHGKPMIFGKNRDKGIRLVGMDTQVVTLGQGVSEKDLLVHDEHCE
                     SPTLAFQLARMAYPRFPTPLGVFRDIEKPSYEALMFDQIKATTAKLGPGDLNKLFASG
                     DTWVVE"
     gene            11516..12298
                     /locus_tag="PLU99_05645"
     CDS             11516..12298
                     /locus_tag="PLU99_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16576.1"
                     /translation="MDSLAASQKQFDHDEPPVSQLLPSISVIIPAFNEAQTIEATLRR
                     ISEYFSGSRRLQEIVVVNDGSTDGTAEVVRNFASDKKYVRLITSNANRGKGYSVRQGM
                     LTGTGDLLLMCDADLSTPIEELEKLLPWLQRGYDIVIASRDLPDSRLELPQPALRRGL
                     ATIFRGLRRLLILRSLRDTQCGFKLFRRDTARKVFGYQRDNGWLFDCEILAVAERLGV
                     RIKEVGVNWRDHAPSRVRLLRESLRVLPALLRLRWRLTRIPS"
     gene            12625..>14985
                     /locus_tag="PLU99_05650"
     CDS             12625..>14985
                     /locus_tag="PLU99_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOO16577.1"
                     /translation="MGREQPLKLMRIVLLLVLACCAGNVNLATASPAANPTRLDDGTQ
                     GWDLSFPVGEFLTYQSDSGRLANETRGHTVLYEETVFVADAMWIRLFFDRAALDKGSF
                     VRITSALDGEIQELDASGMTMWQNTSAYFNGDTVYVELVGAPESRGNQIVLGRVAVQM
                     RPADRGPCAADDCGICGEDDRVPSGELWSCRLMQVGCSASVYNMNSCLVSAGHCVDTI
                     WPLVAQFNVPFSESDCDPVHPPVADQFPCTARQYLNNGVGGDWAVLTTGTNGLGQTIY
                     DRYGQFRPLASAPVPQGTPCAVWGYGVDNSDPTRSQSQQYSSGTIVSRHSSYYEYNAD
                     TTYGNSGSGLLNANNEEIVGIVSHCTLNCPNVATRIDRQDFVDARELLCPTVSYCTAS
                     ANNTGYEHISSVTVGSISQSSGSDGYHDYSALSTDMYIGTGYPLTVTIGSPYDSDIGG
                     VWIDWNQDRDFDDANETITTAWTGAGPYTTTITPPEGAYFGFTRMRIRVQDSDYDPAL
                     TPCGDTEYGEVEDYTVHVIRFVDTTPPTPNPMTFDVPPVAASLTSITMVATTAVDEHS
                     PPVAYAFDFVSGGTGGDSSGWQLSSAYTDEGLQINTMYTYRVRARDSAQPSNITAPSD
                     NFTAATAIETPEYISFGLTTTTSIPLNAQGFFTKLTYEQSGLYFDSTTPGGDGGINEW
                     IQTTGDTATGLTPNTTYSFRVKARNQVGTETPYCESAVATTRIETPTGVTFGTISSNS
                     IELIATGTFTNLTEESSGLYFDSTTTGGDGGINEWVQVTTDTATGLS"
BASE COUNT         2865 a         4802 c         4686 g         2632 t
ORIGIN      
        1 caaccgccga tggtgttgac cacgctggcc catcacatcg acctggaatg gctgcgtgag
       61 gcctttcggc gcacgcgcaa ggatggcgcg atcgagcgcg caaaaagcat gagtatctga
      121 tcacctgccc ccaacacgcg atcctggcgg gctttttatg atcgggttgc ccaagctagc
      181 gcttcgggct cggattgctc agggtgctgc ccgcgcagtc gcttcgggct cggatcgagc
      241 ggggggctcg catgggggga tcgggttgcc ctcgctagcg cttcgggctc ggatcaagcg
      301 ggcgacgttg gtggcggcaa tccggggcga cacgccccgg ctctgctctt tctccctcga
      361 tccctcgatc cctggaccct tggatcccct gggctgtgcc gcgccttcgc gcatccgccg
      421 cgagcgggta cgctacacgc catggtactc ttgttgaacg cacagcagct tcaggagaag
      481 ctcgaaaacc tggcccgcgc catcgcggcc gccgctccgc gcgatgtgcc ggtggctgtc
      541 gtcggcatcc gccgccgcgg cgaaacgttg gcccgccgcc tgctgcccct gctggagcgg
      601 gccggcctca agcccgcgca ttatggggcg ctggacatca cgctttaccg tgacgatctg
      661 actacgatcg gtcccagcgc cgtggtacgc gagacggaaa tcgacttcga catcaccgat
      721 acctggctcg tgctggtgga tgatgtgctc tacaccggcc gctccgtgcg ggccgcgctc
      781 gacgccctga ccgacctggg ccgccccaag gccatccgcc tggcggtcct ggtggatcgc
      841 ggcctgcgcg aattgcccat ccaggccgat ttcgtcggcc tgcgaaccga cacgcagccg
      901 caacaatccg tggatgtaag attgctcgaa gtggacggcg tagacgaggt cgaactcaat
      961 ggccgccgct aaaagcgccg aacgccccgt gtggacgcgt aaacatttgc tcaccttggc
     1021 ggaactcacc cccgaggaaa tcttgcacat cctcgacacc gccgaaggat tcaaggccgt
     1081 ttcgacgcgc agcgtcaaga aagtgccggc cctgcgcgga cgcgtcgtgg tgctgatgtt
     1141 ctttgaggag agcacgcgca cgcgcatgag cttcgagctg gcggcccaac gcctctcggc
     1201 cgacgtgatc gagttctccg aaaagatgtc ctcgaccaag aagggcgaaa ccctgctcga
     1261 cacggcccgc aacatcgagg ccatgggagt ggacatcatg gtgctgcggc acggtgcggc
     1321 cggagcggcc aatcacctgg cccaagccac caagtgctgc gtggtcaacg ccggcgacgg
     1381 ccagcacgcc cacccgacgc aggccctgct ggacctgttc acgatccgtg agcgcaaagg
     1441 gcgcatcgcg ggtctgaatg tggccatcgt gggcgacatc acgcactcgc gcgtggcccg
     1501 ctcggatttg cgtggccttc agaagctggg ggcgcacgtg acgttcgtag gcccgccgac
     1561 gttggtgccc aagagcttcg aaaagctcgg agcgcgcgtc acgcacgatt ttgacagcat
     1621 tctgccggag atggacgttg taaatatgtt gcgcatccag aaagagcgca tcgccagcaa
     1681 cgtctttccc tccatccgcg aatacgtgcg gttgttcggc atgaacagcg agcgtctcaa
     1741 gcgctgcaag ccggacgtgc tgatcatgca ccccggcccg gtcaaccgcg ggatcgagct
     1801 ggcggccgac gtggccgacg gaccgcattc cagcatcctg cgccaggtga ccaatggcct
     1861 ggccgtgcgg atggcggtcc tgttcctatg ccagcaggcg gcgatgccgg acgtggaagc
     1921 ttagcggccg cttttggggg agtggccgtc cgcgagcgcc ccgacgcggc tcgacaacgc
     1981 cgcaacagtt cgttcttgcc atgaagaccg ggctactccc gatgagtatg atcatctgcg
     2041 atcacccgtg gctacggttt tctagcggag gtccgacatg cccgaaatca cactggaaca
     2101 ggcgcaggcc gtcgtaaacg cggccttgga aagagctcgc ggcatgcagc tcaagatgaa
     2161 catcgccgtg gtggactccg gcgccaacct caaggccttt gcccgcatgg acggggcctg
     2221 gctcggctcg attgacatcg ccattcgcaa ggcccgcacc gcccgcttct ttgacatgaa
     2281 caccggtgag ttgggaaagc tctcgcagcc cggccagccg ctgttcaata tcgagcattc
     2341 gaacggcggc ctcatcacct tccccggcgg cgttccgctc aaagcaggcg ccgagatcat
     2401 tggcgcgatc ggcgtttccg gcagcacggt cgagaatgac cacgccgtcg caacggcggg
     2461 cgccgctgcc atacaaacgt gagtcactga aaccgacgta ctgcgcgatc cacgccgtca
     2521 ggggccggtg gaaatgcgcc accagtattc atcgctctgg tcgagcgtga agcccggcga
     2581 ccatgtgatc tccgtgcaga cgcccgcgat gattaccacg tttccgccgg tccggacgaa
     2641 ttccgacagc tcctcatcgg tcaggtccgg ccggcgggca gtgaggcaga cacgcccatt
     2701 gaagatctgt attccgggcg ggccgccatg cacgagatac cagtaggcgg tcgtcggggc
     2761 agtggtgctt tcgccaagca gccagcctcc ggggccggta cgctcgagcg cttcgcacgc
     2821 gaattcggcc gcggagtgat cgccgcgcag ccagggacgg aagaagtggt tgtactcgtc
     2881 gcgatgcggg cggtgaagcc agcggccttc catccactca ccctggcggg caaggattgg
     2941 gaaggccaca tagaccagcg gcggcagcac ggcgttgacc gccagcagaa cggcgcaggc
     3001 ccgccgcgcg gtaagcgacc gcaaacgccg ccagatcgag gcgacgctca gtccaaacca
     3061 cagcgcaacc atcacatcca gcggaacgaa gaatgtatac agatcggcga tgcggtagcg
     3121 catcacaaat accgtcatca acacggtctg ggccaataga acccagcgga agagcttctg
     3181 cgggccctgc atcggtagca gcagccctag cgctgctatc aacagcgtca aagacggaaa
     3241 gttgtagccg agcgaaagaa acgcgatttt cagttggctc gcggataggt tgaggccgac
     3301 tgccgcctgc gcccagccgc cgtactcggc gccgccgccg aaaaaggtcg agtgcagcgt
     3361 atcgcgcagg tcgtgcgttc ttgcatagaa gtcgagcacc atcgtccagt agggggcgct
     3421 gcccgcgatc cagacggccg cgcaggccag cagccagccc gggcgcagct tgcgccgcag
     3481 gctgcgctcg atcagcagga ccccataggt cgccagtgga atcagcccca gcaggtggtt
     3541 ggcaatgtgc agcccgttaa gggcaaacac cagcaccagc cactgcgggc ggccggtgcg
     3601 cgcgtactgg agcagcgcca gccactcgca gaccatcagg gtggccgcga gcgtgtacgt
     3661 ctccgtggta cacgacattt gccagtagct gtgcgcgacg agcagcacgc cggccgccag
     3721 aaacgcagcc ggccggctgt ttgtgagccg cacgaccaag gcggccagca ggccaatggc
     3781 ggccgcccca cagatggccg aaaccatgtt catgcgccag gccggctcgc cgaacggcac
     3841 cgccagcgcc aaccgcgcca gccggaagtg cagcgggtgg gacagcgcca gcccgcaagg
     3901 gtgttccatt tgcccggtga gaatgcggaa ctggtggcgc cccggatcct gccattcgac
     3961 gcccggtgat acggatgccg cgtatatcac cagtgccagc gcgccgcata gcaggcccgt
     4021 cggccagctc ctgtgttgct ggatttcaga tggcggcgcg ggctcgctga cggcgacttc
     4081 agtaccctga cgcatctgag tgacccttgc cttggttctt cagcgattcg cgcagaaccg
     4141 tgtccaactc ggggtactgt atcttcccct ccagcggccg aatcccgacc gcacgcaagt
     4201 cccccggctt tacgaccacc atgagcgggt tacgtttgaa cccaaaacgc tgcgcgatcg
     4261 ggtccgtcgg ctgcacctcc aggcggcagc aggtgagctg ggccattttt tcgcgaatgt
     4321 cacgttggga aaaaaggtcg cggtccagag ccgcggcaat cgggcagccc ggcgcctgaa
     4381 gcatgaggaa gattagttgg ttcgattcgc gggcgtgttt ttcggcctgc ggcaggtcgc
     4441 gctcccaggc gaccggcgcg ccggcccgtt gccgccattg catggccgca aaccagcagg
     4501 cgaacaaggc cagcaggccg aggatgatcc aacgttgcgg ctggcgctga cgcatggcgg
     4561 gcgttgtaac ggcttcgggc agaggttcgg gcgggtctgt tacgggcaca ttcactcctg
     4621 ccgcagccgg ctcgtggttc ggaggtcaga gtcgccggcg gccacgcgcc gcggtgctgc
     4681 gcgtcacgcc ggcgccgctg aaaaaaccga atttaccgcc ccgcgggccg ggcgtaaata
     4741 tcagccgcgg cggccggcgc accatgcagc ggcggcacat gcacgataca attcggtgac
     4801 cgtgataatg gaggccactg tgctgcgtca gtttccggcc gaacttgaag cccagcgtcg
     4861 cgtgatcgag gaacgtgccc gccaggaggg actcgatttc ttcgagacca tcttcgagct
     4921 ggttgatttc gaccagatga accaggtggc tgcctacggg gggttcccac agcgctaccc
     4981 gcactggcgc ttcgggatgg agtacgagcg gctgcgcaag cagcacacgt acgggttggg
     5041 caaaatttac gaaatggtca taaataatga cccgtgctac gcctacctgc tgaccgacaa
     5101 cgagttagtc gatcagaaga cggtaatggc gcacgtttac gcgcactgcg atttcttcaa
     5161 gaacaacatc tggttcggca aaacgaaccg caagatgatg gacggcatgg ccaaccacgc
     5221 cacgcgcatc cagcggcacg tggagcgcga ggggctggag acggtcgagc ggttcctgga
     5281 cgtgtgcctt tcactcgaga acctgatcga cccctactcg caattcatgc ggcgccagcc
     5341 cgagccgcag ccgcgcgaga gcgccatccc ggccgagcgc cagccggacc gggcggtttc
     5401 gcgttttccg gccaagccct acatggaccg ctacattaat ccgccggaga tgcttgaggc
     5461 cgagcggcag cgggaccggc gcgagcggga gttgcagcgg cgggcctttc cggcggcgcc
     5521 cgagcgggac gtgctggcgt tcctgctaca ccatgccccg ctggaggtgt ggcagtcgga
     5581 catcctggac atcgtgcgcg acgaggccta ctactacgcc ccgcaaggcc aaaccaagat
     5641 catgaacgag ggctgggcca cctactggca ctcgtacatg atgacccgct accacttgcg
     5701 cgatcacgag gtgatctcct acgccgacca ccattcgggg acgctggcca cctcgcccgg
     5761 ccggttgaat ccttacaagt tgggggtgga gctattccgc gacatcgaag accgctggaa
     5821 ccgcgggcgc ttcggcaagg aatacgaaga gtgcgagtca atcgagaagc gccgcgcctg
     5881 ggacaccggc ctacggaagg gccgcgaaaa gatcttcgag gtgcgccgcg ttcacaacga
     5941 cgtgacgttc atcgatgagt tcctgacgcc ggagtttgtt gaacggtata agctctacca
     6001 ttaccgccag gatcccagca ccggccgcat ggtcgcagtg gatcgtgact tcgaccgcat
     6061 caagcagacc atgcttttca tgctaacgaa ccacggccag ccgtacattt acatagtgga
     6121 cgcaaactac gccaatcgcg gggagctgta cttggcgcat aagcatctgg gcattgacct
     6181 ggacatcaag tacgcggccg agtgtcttaa gaatttgcat cgtctgtggc gccgcccggt
     6241 ccacctgcaa gcccgcatca aggacgacat gacgctgttc acctgcagcg gcgaagacgt
     6301 aaagcagcag aagatcaagg acgacatgcc gcgcccggtg cataccaaca tcgagtgatt
     6361 ccgctcgctt attgctgcgg gcgggggctg gcggccgact ggtgcatttt cgacaactga
     6421 tccggcgatt gaatctcggc cagcggcgcc atcacgaatt cgcgctccca catgcgcggg
     6481 tgtggaatgg tcaagtccgc ctcggtaacg cgctgatcgc ggtagatcag taggtccaaa
     6541 tccagtgtgc gggccccgtt cttgacgttc cgtacgcgcc cgtgcctcgc ctcgattgcc
     6601 agcatgcgtt ggaggagggc atgtggcggc aactgcgtat ccagttccgc cgcaccattc
     6661 aaataccggg gttgccccgg cgggccgccg actgggtctg tttcgcgtag actggagcag
     6721 cgcagcacgc gaatgtcgcc ctgctcctcc agttccgcca gggcggtgcg aatatgccgc
     6781 tcgcgatcgc ccaaattcga gcccatcgcg atgtatatcc cgccctcagg catcggctca
     6841 ccagtgcagc ttacgcagcc gtgcgatcgc cgtcgcgatg cggtccgtat cgaccgttaa
     6901 agcaaagcga acgtaatcgg cagcgccctc gccaaagccg atgccgggaa tgcacaccac
     6961 gaacgcctcg tccagcagct tgttcgccac ctgcatcccg tcgtagcccg ccggcacgcc
     7021 ggcccaaacg tagaacgtcg ccttcggcgg gtcgacgcgg aagccaatcg agcgcaggcc
     7081 ggcgcacaga acctcggccc gctggcgata cagctcgcgc gtggcggcga tctccgggcg
     7141 gtcgataccg gcgtatgctt caatgccggc ctcctggatc gcaccgaacg cgccgctgtc
     7201 caggttcgcc ttgaccttcg ccagggcagc cagcacatcc ggcctgcccg ccgcgaatcc
     7261 cagccgccag ccagtcatgt tgaaggtttt tgacagggag tggaactcga ccgcgatgtc
     7321 gcgcgccccc tcgacttcca gcacgctcgg cggacgctca ctgccaaggt agacctcgct
     7381 gtaggccgca tcgtgcgcca gaaccaaatc gtggcgacgt gcgaactcga cggcgcgctc
     7441 aaaaaacgtt cgcggcgcca ccgccccggt cggattgttg gggtagttga ggaacatcaa
     7501 aaccgccgcg cgcgcgactt ccgctggaat ggcatcgaac tggggcaacc aatcgttctc
     7561 cgccgataac tgcatcgcgt atggcaaccc gcccgcgaaa agcgttgccg cccggtacac
     7621 cgggtacgcc ggatcgggaa tcagcaccct ttgcccaggt tccaccaccg ccagcggcag
     7681 atgccccagt ccctccttgc ttcccaccag cccgagcact tcccgctccg ggtccaagtc
     7741 cacgccatag cgccgccgga aaaagtccgc cgcctcttgg cgaaactccg gcaatccgcc
     7801 gtcgggtggg taacggtgat gcacgggatc gcgcaccgca cgctgcaacc ggtcgattat
     7861 gaagccgggc gtcggctggt ccgggtcgcc caccccgaaa tttattacat catgacccgc
     7921 cgccagcgcc gcgcgccgct tgcgatcaat atcgataaac aggtacggcg gcaggctcgc
     7981 cagccgttgc ggcaaggaga aagccatttt tcatctcgtt ttgcgtcggc caccagtcta
     8041 cggatgttag ctaaccgcga caagccgagg ttggaacgcg agttgccagc ctgacctctg
     8101 acgagcgcgg acgatcctcg ctgccgcatt taactgcggt ggccggcctg tgcggccgtc
     8161 cttgactgga atttcccgcg tcgctgggct aaactgcgtg gttttacgcc cgacagggtg
     8221 agaggtgagg tctcggccca caatcgaagg actcccaagc taccccggac ttttccaagc
     8281 ccgccggcag ccgcgcccgg ggcatgcgcg tgtcggcaga gagccaagtt gaatgagtac
     8341 cacgacttcc agcaaaaaag ccatggcaac tactggcaag cgcactgaaa cgctcgaaca
     8401 ggctgtgatc cggttcgcgg gcgattccgg cgacggaatg cagctcgtcg ggacccagtt
     8461 cacgacggcc agcgccgtgg tcggcaacga cgtcagcact tttccggatt acccggccga
     8521 aattcgcgct cctgcgggca cgctgggcgg tgtgtcgggc tttcagatta acttcggcag
     8581 ccgcgcggtc tacacgcccg gtgacaccgt ggacgcactg gtcgcgatga acccggcggc
     8641 actaaaggtc tgtctcaaag acctcgcgcc gggcggcgta ctggtggtca actcagacga
     8701 gttcaccccc atgaatctgg ccaaggcgcg ctacgaaacc aacccgctcg aaaccgacga
     8761 gctggacaag taccgcgtct acaaggtgcc catcaccagc cagaccctgc tggccgtcaa
     8821 agaaaccggc ctgaaccaga aggccgccga gcgctgcaag aacttttacg cgctgggcgt
     8881 ggtgtactgg ctgttcgacc gcccgctgga cgtgaccatc cgctggatcg acagcaaatt
     8941 cgccaaagtg ccggccgtcg cggaggccaa ccgccgcgcc cttcaggccg gctacaacta
     9001 cggcgaaacg gccgaaatat tctccgtccg ctaccgcgtc ggcaaggcgc cgctgaaacc
     9061 cggacgctac cgcaacgtga ccggtaacca ggccgccgcc ctgggcttcg tcgccgccgc
     9121 caagctcgca ggcaagcccc tgttttacgg cagctatccg atcacgccgg ccagcgaaat
     9181 tctgcacgag cttacgatct acaagaactt cgacgtgcgg acgttccagg ccgaggacga
     9241 aatcgccgcc tacgcggccg tgctgggcgc ggcctacgcc ggctgcatcg ccgttaccgg
     9301 caccagcggc cccggtttgg cgctcaaaca ggaaggcatc ggcctgggca ttatgacgga
     9361 attgccgacc gtcatcgtgg atgtgcagcg cggtggtccg agcaccggtc tgccgaccaa
     9421 gaccgagcag tccgatcttt ggcaagctat gctcggccgc aacggcgact gtcccgcgcc
     9481 cgtgctggcg gccagctcgc ctgcggactg tttctgggct gcgatggagg cggttcgtat
     9541 tgccgtcaaa tacatgacgc ccgtgttcct gctcagcgac ggctatctct cgcagggctc
     9601 cgagccgtgg cgcatcccga atctggacga gctcgcgccg atcaaggttg aacaccccac
     9661 cgatccggcc gatttcgcgc cttaccgccg cgatgccaac ggcagccggc cctgggccat
     9721 tcccggcacg ccggggctgc aacatcgcgt gggcgggctc gagaaactgg acgtcgctgg
     9781 aacggtcagc tatgacccgg ccaaccatca gcggatgacc gagctgcggg ccggcaaggt
     9841 cgaaaacatc gcggccgaaa ttcctccgca ggaagtttac ggagatgaag ccggcggagc
     9901 tttgttgctg gtttcctggg gcggcagctt tggggcagtt cgctccgccg ttgagcgagc
     9961 ccgcgaagac ggccaagacg tttcgcacgt tcacctgcgt tggcttaacc cgatgccgcg
    10021 caacctgggc gagattctgt cgcgcttccg aaccgtcgtt gtctgtgaaa tgaacatggg
    10081 ccagttgcag atgttgctgc gggcccgcta cctcgtggat gcctatggcc tgcacaaggt
    10141 ccgcggtcgc ccgttcatgg tgagcgacat ccgtgccaag atcgacgaag ttctgggagg
    10201 gcgctaacca tgaccacgat ggctgatcat cccgtgctta ccataaaaga ctacaagagt
    10261 gaccaggacg ttcgctggtg tcccggctgc ggcgactttt cgatcctcac ggcgattcag
    10321 aagtgcctcg ccgcccggca ggtgccgaaa gagaaggtgg tctgtgtgtc cggcatcggc
    10381 tgctccagcc ggttcccgta ctacatggac acctacggca ttcacagcat tcacgggcgc
    10441 gccccggcgg tggcgaccgg cgtgaagctg gccaaccccg agctggaggt gtgggtgatc
    10501 acgggggacg gcgacgcgct gagcattggc ggcaaccact ttctgcacgc cattcgccgc
    10561 aacatcggtt tgaagatcgt gctcttcaac aaccgtattt atgccttgac gaaggggcag
    10621 gtttcgccga ccagcgagca gggcaagcgg accaagtcca cgccctatgg ctcgatcgaa
    10681 aaccccgtgc actcgatcgc ggtcgccctc ggcgcggaga tcaccttcgc cgcccgcgcg
    10741 atctataacg acccgaagat gctccaggat attctcgagc gcgccgcgaa ccacaagggc
    10801 acggcgttcg tcgaggtcta ccaggactgc gcggtgtaca accacgaggc tttctcgtac
    10861 ctgactgacc gccagacgcg ggacgacaac atcctctacc tcgagcatgg caagcccatg
    10921 attttcggca aaaaccgcga caaaggcatt cgcctggtgg gcatggatac tcaggtagtg
    10981 acgctcgggc agggtgtttc cgagaaggac cttctggtac acgatgagca ttgcgagtcg
    11041 ccgacgctgg cgtttcagct cgcccgcatg gcgtacccgc gtttccccac gcccctgggc
    11101 gttttccgcg acatcgaaaa gcccagctat gaggctctga tgttcgatca gatcaaggcg
    11161 acgactgcaa agctcggccc cggcgatctg aacaagctgt ttgcctccgg cgatacatgg
    11221 gtggtcgagt aggcggttcc ggccgcagcg gctcgatctt tatcagccga cgctcgtccc
    11281 gcaggtcgct cttcgtaagc tgcggcgcgc tcccgcacag cgccgcagcg gggcggggcg
    11341 cgcctgtggc gaagcgccgg acgtgacgcg aattcaggcg tgtcccgagc cgcgccacgg
    11401 cgcccccagc catttgagcc gggcctttgg acagttgctc cacagaccgc ctgccgctat
    11461 tttccctcgt gcactcgcgg cacgatcgac gcgacccagg atgggtgaat cagtcatgga
    11521 tagcctggca gcctcgcaga agcaattcga ccatgatgaa ccaccggtca gccagttatt
    11581 gccgagcatt tcggtcatca ttcccgcctt caacgaagct cagactattg aagcgaccct
    11641 gcggcgcatc agcgaatact tttccggctc ccggcgcctg caggaaatag tggtggtgaa
    11701 cgacggcagc accgacggca cggccgaggt ggtgcgcaac ttcgcatcgg acaagaagta
    11761 cgtgcggctg atcacgagca acgccaaccg cggcaaaggc tatagtgtgc ggcagggcat
    11821 gctcaccggt accggcgacc tgttgctcat gtgcgatgcc gacctctcca caccgattga
    11881 ggagttggag aagctgctgc cctggctaca gcgcggctac gatatagtaa tcgcctctcg
    11941 cgatttgccg gattcgcgcc tggagcttcc gcagcccgct ctgcgccgcg gattagcgac
    12001 cattttccgc ggtctgcggc ggctgctgat tctgcggtcc ttgcgcgaca cgcagtgcgg
    12061 attcaagctg ttccggcgtg acacggctcg caaggtgttc ggataccaac gggataacgg
    12121 ctggctgttc gactgcgaga ttctggcggt ggccgagcgc ctgggagtcc gaatcaagga
    12181 agtgggcgtc aactggcgtg atcatgcgcc gagccgcgta cgcctgctgc gcgagagcct
    12241 gcgcgtgctg ccggcgctgt tacgcctgcg gtggcgacta acgcgaattc cgagctaggc
    12301 gactgattgg gctacggaac cgcccgataa cgcgggcccg atatggagtt gtagcctggt
    12361 ccgccgcggc cagcggccta tgggcaggta gcttatctga cccaagaggc gggacgcgga
    12421 cgtccgcaag ttggggtttc aaattctgtg ttaatccgct attgtagcgt tgcgctgttg
    12481 gcgatgggcc ttatagaatc gcacggagtt tttcgtagcg gcgtcgccgg aacggagccg
    12541 ttgcgccgtg cgccgttttc gccaaaggcg cgctgcgggc ggtggctatc cgcggcaggt
    12601 tgggaaagct atcggaggaa cgcgatggga cgggagcagc ccttgaaact catgcggatc
    12661 gttttgctgt tggtgctggc ctgctgtgca ggcaacgtta atttggcaac tgcttcgccg
    12721 gctgccaatc ctacgcggct cgatgacggc acgcagggct gggacttgag ctttccggtg
    12781 ggtgagttcc tcacgtatca atcggacagc ggccggctcg cgaacgagac gcgcggccac
    12841 accgtcctat acgaggagac cgtttttgtc gccgacgcca tgtggatcag gttgttcttc
    12901 gaccgggcgg cgttggacaa gggcagcttc gtgcgcatca cgtcggccct ggatggcgag
    12961 attcaggaac tggatgcctc agggatgacg atgtggcaga acacgtcggc ctacttcaac
    13021 ggcgacacgg tatacgtcga actggtgggg gcgcccgagt cgcgcggcaa ccaaattgtc
    13081 ctgggacgtg tggcagtgca aatgcggccc gcggaccggg gtccgtgcgc ggctgatgat
    13141 tgcggaatat gcggcgaaga cgaccgtgtg ccctcgggcg agctctggtc gtgccggctg
    13201 atgcaggtcg gatgcagtgc gtcagtctac aatatgaata gttgcctggt ttcggccgga
    13261 cattgtgttg ataccatctg gccgctggtg gcgcagttca acgtgccctt ttccgagagc
    13321 gactgcgacc ccgtgcatcc gccggtggcc gaccagttcc cgtgcaccgc acggcagtac
    13381 ctcaacaacg gggtgggtgg cgactgggcg gtcctgacta ccgggacaaa cggtctcggg
    13441 cagacgattt atgaccgtta cggccagttt cgtccccttg cctccgcgcc cgtgcctcag
    13501 ggcacaccct gcgccgtctg gggctacggc gtggacaata gcgatcccac ccgcagtcag
    13561 agccagcagt atagttccgg caccatcgtg agccgccaca gcagttacta cgagtataac
    13621 gcggacacca cttatggtaa ttccggttcc ggcctgctca acgcgaataa cgaagagatc
    13681 gtgggtatcg tttcacactg tactttgaac tgcccgaacg tagccacgcg catcgaccgc
    13741 caggattttg tggatgcgcg cgagttgctt tgtccgacgg tctcttactg cactgcgtcg
    13801 gcaaataaca caggctacga gcacatcagc agtgtgacgg tcggcagcat cagccagtcc
    13861 agcgggtctg acggctacca tgactatagc gccctttcga ccgacatgta cattggtact
    13921 ggttatccac tcacggtcac gatcgggtcg ccgtacgaca gcgacattgg cggagtgtgg
    13981 atcgactgga accaggaccg ggacttcgac gatgcgaatg aaacgataac cacggcctgg
    14041 acgggcgcgg ggccgtacac caccacgatt acgccgcccg aaggggcgta ttttggcttc
    14101 acgcgcatgc ggatacgcgt tcaggacagc gattacgatc cggccctgac gccgtgcggc
    14161 gacacggaat acggcgaagt ggaggactac acggtgcacg tgatccggtt tgtggacacc
    14221 acgcctccca cgcccaatcc gatgacgttc gacgtcccgc cggtcgccgc cagcctgacc
    14281 tccattacca tggtggcgac cacggcggtc gatgagcact cgccgccggt ggcctacgcg
    14341 tttgatttcg tgagcggggg cacgggcgga gacagcagcg ggtggcagtt gtccagcgcc
    14401 tataccgacg aagggctgca aatcaacacg atgtacactt atcgcgtgcg ggctcgcgac
    14461 agcgcccagc cctcgaacat aaccgccccg tcggacaatt ttactgctgc gaccgcaatt
    14521 gaaactcccg agtacatcag tttcggcctt acgacaacga cgtcgatacc gttaaacgcc
    14581 cagggcttct tcacgaagct tacttatgag cagtccggct tgtacttcga ctcaaccacg
    14641 cccggcggcg acggcgggat caacgaatgg atccagacaa ccggtgacac tgccacgggc
    14701 ctgaccccta ataccaccta cagttttcgc gtgaaggccc gcaaccaggt cggtaccgag
    14761 acaccctact gcgagagcgc agtcgcaacg acgcgcatcg aaacgccgac cggggttacc
    14821 tttgggacta tcagttcgaa cagtatagaa ctcatcgcga ccggcacttt cacgaatttg
    14881 accgaggagt cgtcgggatt gtactttgac tcgactacca ccggcggcga cggcggcatt
    14941 aatgagtggg tccaggttac caccgacacc gccaccggcc tgagc
//
LOCUS       DAOSYS010000064        14849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3402_length_14849_cov_5.646208, whole genome shotgun sequence.
ACCESSION   DAOSYS010000064 DAOSYS010000000
VERSION     DAOSYS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3402_length_14849_cov_5.646208"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1483)
                     /locus_tag="PLU99_05655"
     CDS             complement(257..1483)
                     /locus_tag="PLU99_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16578.1"
                     /translation="MSYSSARLMAAAFRSVGVDAEATPDSNGETLELGGLYSSGEECL
                     PHKITLGDFLRICRRPGAQAARLAFFMPRAQGPCRFGQYAPYLKQVLEQEGYGEALIL
                     SPSSASNYDELGDHASQLMRTTWMGIVVSDLVSRYLLKTRPYELRAGDTDEVFRDSVE
                     RLCQILSRRDLTTKERLRALTAENAAMRERFHAIPARYEKGRPFIGLVGEIFCRLNTF
                     SNENVARRIEQLGGECWLSDISEWIWYTSWSRETDCIRDNGRLNWTFLKLKLKTRLQQ
                     VYEKALLAPVHDDFKGYEEPHDIREILRCSEPYLPATGALGEMTLSVGKAIYLYNKGI
                     DGVIDISPFTCMNGIVSEAVYPAVSAAHDNLPIRSLYFDKVSTKIERDLEIFLDLARA
                     YQRRKTRPRTYPDYFK"
     gene            complement(1459..4641)
                     /locus_tag="PLU99_05660"
     CDS             complement(1459..4641)
                     /locus_tag="PLU99_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOO16579.1"
                     /translation="MRVDFYVGIDVGAISVKVALLASGSTSNPDGSEPGRIASEPEFH
                     NPLRQIQTPGAVPPISVAAPKRTRGRPLEAVREALEEVLGALPADSALRVAITGSGGK
                     LVAQALGAPVFNEFQACARAVDRLMPQARTVFEMGGECSRYLRLLPDPSSRRLGIVDY
                     SSNGDCAAGTGAFLDQQAARLQYAVEDIATIVAGAERAAQIAGRCSVFAKSDMIHAQQ
                     KGFKPPEVLRGLCNAVATNFKSAVVKGRPVEPPVVFVGGVAANSAVVAALRETLALGE
                     NELCVPEAPESYGAIGAAVLAMESRNCPSRRPSEWLEALQTALRGDGSAFAATRPLNL
                     DRVKLLRDQVRPYRFPDRTPVDAYLGLDIGSVGTKLVLIDSRGDVIYSIFTRTEGRPV
                     EVVTRCLRELEEAVGWGVRVRGVGSTGSGRELIGELVGADSINDEITCHKTGATFVGD
                     RLLGKRPDTIFEIGGQDSKFISLQPEGGDSRETIVVDFTMNEACAAGTGSFLEERATE
                     LGVSIKDEFSRLALSSQAPIRLGERCTVFMERDVNTCMQRGAQRADIIAGLAYSIVYN
                     YINRVVRGRPIGDCIFFQGGTAYNDAVAAAFSIVCDKEIIVPPHNAVLGAIGAALLAK
                     EKIARTGATTRFRGYDLEQVEYSLREFTCKGCGNLCSIQEFNVAGEKTYWGDKCSERY
                     RKQVKTDRKPIIPDLIARRFELLNADDAGDPPHPRGSIGIPLAMYTWDWLPLWRRFFR
                     DCGFQVIVSSETNRQTVRQGLDSVVAEPCFPIIVAHGHIAELVSRNVDYLWIPNLITV
                     PGRDSHLENYVCPWGSTLPFVIRHNPAFRGWADRILFPLLSLQRGTDVLRSDMVRVAA
                     KLGVPAKTARRAFDAAWAAQQRFLSAYQEAGRAALQRLIDTRESGMVLVGRPYNSHDA
                     GVSLAIARKLREQYGVNLLPVDALPLAGIDVTDVNDNMYWAYGRRILAAAKLVREHPN
                     LHIIYVTNFKCGPDSFVKAFIRKASGKPFLTLQFDGHSNDAGMLTRCEAYLDSKGILR
                     WWRTQQTKSIHELLPAAQSISPKCPTPVRG"
     gene            4981..5961
                     /locus_tag="PLU99_05665"
     CDS             4981..5961
                     /locus_tag="PLU99_05665"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16580.1"
                     /translation="MFRKTRPAFVVATLILTSLPAGAWASDPDPQPLFIMRDGRRVPV
                     AVIPARSAPGNNSRQAVLEVVEAGQVVPPQAGVARPRERPGDEWGGAGRAGGAPPRRQ
                     EIAYYAGYPVSYYYPTDTSWWDTGPIYPNPYNTDYGVAVEQLYRGQRYVYMRERGRRF
                     NEVDMQQRRERALTNSETALRTGLDDLKSGAYEQAVVALTMAGQLNQGDPASRIHLAQ
                     AQMALGHYDEAAAALRRALQLQPNLKFIPLNLGKYYSDPEDFDLHVQRLYEYVRGHAV
                     TADVYFLLGYMQFQREDFAQANAAFQIALRGAPNDALVREYVKLTKPAAR"
     gene            complement(6873..8327)
                     /locus_tag="PLU99_05670"
     CDS             complement(6873..8327)
                     /locus_tag="PLU99_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOO16581.1"
                     /translation="MFGGEDVQITPGMQRYIDLNNAYLAKRDKYIREVVKNWKFDTIA
                     VHGLYTVQDAIEDYQGAIIEPIFMSSSQAYRDSDEMAAALAYLIPTWCYSRIANPSTY
                     YYEWTLALLEGYGFDGETSCCSTSSGMAAIMTAVQPFLVHKRRHVHEARNFVAVAQCY
                     GGTFQQFSVRLMEERDIECRWVQNPENIDEWASKIDKNTRFLYGELPSNPGQSFFDIA
                     AVADLAHSHNLPLICDSTVATPALLRPLCHGADIVVQSATKSITSSGFGVCGALIARK
                     NLVTNIDNEDLKKDFALYVKYLPNRDYGPNLHPMQAIMTLNDVRTLRSKMDLLSRNTM
                     KVAQFLSKHPQVESVQYLGLPEHPLHELASKYMWLVDAEYDEQYGKPVNRYGHLMSFC
                     VKGGPEAARKFFDGLQRIWRATDLGRIKSVATIPAISTHQQQGEAGRKLANIPPNLVR
                     LCVGGEHPDDIIADLDQALSKLQTKAAVPARAHG"
     gene            complement(8551..8955)
                     /locus_tag="PLU99_05675"
     CDS             complement(8551..8955)
                     /locus_tag="PLU99_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16582.1"
                     /translation="MKPTAEPPAELPEVHCVREVRLEIPQELLPEVQEFYTDLLGLRP
                     WPAAEQIPGGWGLGNPARGLYLQFRHDPHVDPVRRRLTLLVPDLPQIIARLNERGWPF
                     EHYHGFGAAEEWLVLADPVGHLIEVRASHRSL"
     assembly_gap    9158..9257
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9258..9718)
                     /locus_tag="PLU99_05680"
     CDS             complement(<9258..9718)
                     /locus_tag="PLU99_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOO16583.1"
                     /translation="MPRTVKSLKHQQASLTPKQLRVLTFIRDYTKARGFAPTMQELAD
                     EFDVSKVTVFEHIVALQKKGFLRRSRHRARSLRLSEGIEFPDERPTLLPLVGTIAAGK
                     PIEALEQPETLDLEEVFVSSRGNFVLRVRGDSMINDQIRDGDYVVVEKRETA"
     gene            complement(10328..11539)
                     /locus_tag="PLU99_05685"
     CDS             complement(10328..11539)
                     /locus_tag="PLU99_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO16584.1"
                     /translation="MPDFASPWIAAWFWITLAIGLVWLRRHLQVNRGGKEHVLSEQDA
                     QTVDQSGGGADSNGWPSLSVVVAAKDEEQNIARCIDGLLAQDYPRLQVIIANDRSRDR
                     TPQIIDDYARRDPRLTAVHVRELPAGWFGKNNAMREAMQQATGELLCFSDADCAYDSP
                     LLLRAAVSFARREGIEFLSVLPRLEANSLWEKVIQPVAGAIMVFWFPPQKVNDPRCRE
                     AYANGAFMLMTRRAYEAIGGHEYAKATLNEDMHMARQAKRVGVRLQVLQSAGLYRVRM
                     YTGLKQIWRGWTRIFYGCFGTLPRLLASVIMLSIFSVSPYVALLASPLIGGSGVWLAA
                     AAAFAIVSQQSILMRFYALTGNAPGWALTYPVGATVCLGMTLNAMTRLTGKTTNWRGT
                     AYAGGAQVKTT"
     gene            complement(11720..12181)
                     /locus_tag="PLU99_05690"
     CDS             complement(11720..12181)
                     /locus_tag="PLU99_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOO16585.1"
                     /translation="MPTPSAGLLMFRIRHGQPQVLLVHPGGPYWAKKDAGAWTIPKGE
                     IAAGEDSLAAAIREFEEETGLKPAGPFIPLGTIRQKGGKLIHAWVFESDCDPAALRSN
                     TFTMEWPPRSGQTRTFPEIDRAAFFTPDEARQKINPAQVELLDRLEEHLRG"
     gene            12441..13754
                     /locus_tag="PLU99_05695"
     CDS             12441..13754
                     /locus_tag="PLU99_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16586.1"
                     /translation="MRTATILIAMTGLLACRSAGAQDITVEQQKLLARRAAEADAYRK
                     LAQIVYGIQLNERTYVQDFVCESDEIRGEVDTFIKGVRLGQPIFYDDGSCEIPAEVTV
                     AKVVETLRTACDRWYKGEDIKTSDFESISRRIQKDVIKVCGMGAPRADLPPDLPQCAM
                     EMLPPGPPIAENAPPPPIPDIWKQIGPQARLSAMRAAQMDAMRKLAERIKGLRLTSQT
                     CVRDFVAESDRITADMQAFLKGAREICCYFHSDELICECTYVVPTEQVITTIKQLHSR
                     YYNGDPDDTRGSDIEKIVKTVVKKDFEATGMGVPNPRFMQKFQESAAVRMPDWSMRTL
                     EADGRGTDPQIDTPQGRLRAARAAEMDARRRLAEQIAGLQLESATTVSDFAMQHDEVR
                     GLVEAVVVDAAVTNTRFDQDVAVVTVALPGMSVWSVVNDGLPRNQ"
     gene            13789..13938
                     /locus_tag="PLU99_05700"
     CDS             13789..13938
                     /locus_tag="PLU99_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16587.1"
                     /translation="MRLFVSLAISLALMLTVGCASQRHHKVRVLEEERVGEVQEAPQG
                     EMIVE"
     gene            13953..>14849
                     /locus_tag="PLU99_05705"
     CDS             13953..>14849
                     /locus_tag="PLU99_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16588.1"
                     /translation="MHVWPQWLLCSSPWACVLPVAIGWAAGALSLQGRALERVALAKA
                     RSELLDRVYALKLRESLTIGDWAAHDLGRDRALRLWLRQTAAYGAARTYSDGSCDVAV
                     RLAPAAIEQKLEALSATSGATADGAVATPDFSLAACEWPVLWAQGTAWPTEAELAQQP
                     EGWEDVTPVGLEMTRRAAEADARRALFDSIGMLPVTRARRLSDFLESGEQVREAVIAA
                     LQREALLAVSFAPDQVAVATARIGLVDLIRILTEVHENNYQGDGFHGRDFRGMGLLAT
                     VDEVSATGWAIPPAAAVSRPHYP"
BASE COUNT         2649 a         4321 c         5042 g         2737 t
ORIGIN      
        1 ggggccgatc ttttctagat gatatagctc gggcacgcgg gcccggaggc atgcaggcag
       61 ggaggggcgc ggattgttga tggttgggcg tggttgtgcg gcagtggcgg cgagacgcct
      121 gcttggggtg ataaagtgat aaggtgatag ggtgataagg gtgcgagcca aggcaagcgc
      181 gaagacgccg agggcgccaa ggccgccaga catcgcgagg gatcaagggg tcgggatcaa
      241 gaggcggcgt ttcgctctac ttaaagtaat ccgggtacgt gcgcggccgg gttttgcggc
      301 gctgataagc ccgggcgagg tccaggaaga tttccagatc gcgctcgatc ttggtgctca
      361 ctttgtcaaa gtagagcgag cggatgggca gattgtcgtg agcggcgcta acggccggat
      421 agacagcctc gctcacgatg ccgttcatgc aggtgaaggg gctgatgtcg attacgccgt
      481 cgatgccctt gttatagagg tagatggctt tgccgacgct gagggtcatt tcgcccaggg
      541 cgccggtggc cgggaggtag ggctcggaac agcgcaggat ttcgcgaatg tcgtgcggtt
      601 cttcgtagcc cttgaaatcg tcgtgtacgg gggccagcaa ggccttttcg taaacttgtt
      661 gcaaacgcgt tttgagcttc agcttgagga aggtccagtt caggcggccg ttgtcgcgga
      721 tgcagtccgt ttcgcgcgac cagcttgtgt accagatcca ctcggaaatg tcggacagcc
      781 agcattcacc gccgagctgt tcgatgcgac gggcgacgtt ctcgttgctg aaggtgttca
      841 ggcggcagaa gatttcgccg accaggccga tgaacgggcg gcctttttcg tagcgcgcgg
      901 gtatggcatg gaaccgctcg cgcatggcgg cgttttcggc cgtcagagcg cgcagacgct
      961 ccttggtcgt cagatcgcgg cggctgagga tttggcagag gcgctcgacc gagtcacgga
     1021 acacctcgtc ggtgtcgccg gcgcgcagct cgtacgggcg cgtttttaac aggtaccggc
     1081 tgacgagatc gctgacgacg atgcccatcc aggtggtgcg catcaactgg ctggcgtggt
     1141 cgccgagctc gtcgtaattc gacgcgctgc tgggcgagag aatcagcgcc tcgccgtagc
     1201 cttcctgctc caatacctgc ttcagatacg gcgcgtattg gccgaagcgg cacgggccct
     1261 gggcgcgcgg catgaagaac gccaggcggg cggcctgcgc gccgggccgg cggcagatgc
     1321 gcaggaaatc gcccagggtg atcttgtgcg gcaagcactc ttcaccgctg ctatagaggc
     1381 cgccgagttc gagcgtttcg ccgttggaat caggcgtagc ttcggcgtcg acgcccaccg
     1441 agcggaacgc cgccgccatc agccgcgcac tggagtagga catttggggg atatagactg
     1501 tgcggccggc aagagttcgt gtatggactt ggtctgttgc gttcgccacc atcgcagaat
     1561 tcccttgcta tccaggtacg cctcgcagcg ggtgagcatg ccggcgtcgt tagaatgccc
     1621 gtcaaactgg agcgtgagaa acggcttgcc cgacgccttg cggataaaag ccttgacgaa
     1681 tgagtcgggg ccgcatttga aattcgtgac gtagataata tgtaggttcg ggtgttcgcg
     1741 gactagcttg gcggccgcca gaatacggcg gccatatgcc cagtacatgt tgtcattaac
     1801 gtctgtgacg tcgattcccg ccagcggcag agcgtcgacc ggcaagaggt tgacgccgta
     1861 ttgctcgcgc agcttgcggg caatcgccag actgacgccg gcgtcatgac tgttgtaggg
     1921 gcggccaacc aggaccatgc cgctttcgcg cgtgtcgatc aagcgctgga gggcggcgcg
     1981 gccggcctcc tggtaggcgc tgaggaagcg ctgctgggcg gcccaggcgg cgtcgaaggc
     2041 gcggcgggcg gtttttgccg gtacgcccag cttggccgca actcttacca tgtcgctgcg
     2101 gaggacgtct gtgccgcgct gtaacgaaag cagcgggaac aggatgcggt cggcccagcc
     2161 gcggaaggcc gggttatggc gaatcacgaa tggcagtgtt gagccccacg ggcacacgta
     2221 attttccaaa tgcgaatccc ggcccggcac ggtgatcagg ttggggatcc agaggtagtc
     2281 gacattgcgg ctgaccagct cggcaatgtg cccgtgcgcc acgataattg ggaagcacgg
     2341 ttctgcgact acgctatcga ggccctggcg gacggtttgg cggttggttt cgctcgagac
     2401 gattacttgg aacccgcagt cgcggaagaa ccggcgccac agtggcagcc aatcccaggt
     2461 gtacattgcc agggggatgc cgatcgagcc gcgcgggtgc ggcgggtcgc cggcatcgtc
     2521 ggcgttcagc agttcaaagc ggcgggcgat caggtcaggg ataatcggct tgcggtcggt
     2581 tttgacctgc ttgcggtagc gctccgagca cttgtcgccc cagtaggttt tttcgcccgc
     2641 gacgttaaat tcctggatgg agcacaagtt tccgcaacct ttgcaagtga actcgcgcag
     2701 agagtattcg acctgctcca ggtcgtagcc acgaaagcgc gtggtagcgc cggtgcgggc
     2761 gattttctcc ttggccagca gggcggcccc gatggcgccg agcacagcat tgtgcggcgg
     2821 cacgatgatc tctttgtcgc agacgatgct gaaggcggcc gcgacggcgt cgttgtaggc
     2881 cgtgccgccc tggaaaaaga tgcagtcgcc aatggggcgg ccgcgcacaa cgcggtttat
     2941 gtagttgtag acgatggaat acgccaggcc ggcaatgatg tcggcgcgct gcgcaccgcg
     3001 ctgcatgcag gtattgacgt cgcgctccat gaagacggtg caacgttcgc cgaggcggat
     3061 gggggcctgc gagctcaggg ccaggcgcga gaactcgtct ttgatcgaga cgcccaactc
     3121 ggtggcgcgc tcttcaagga agctgcccgt gcccgccgcg caggcctcgt tcatggtgaa
     3181 atcgacgacg atggtttcgc gcgagtcgcc gccctcgggc tgtaggctga taaacttcga
     3241 atcctggccg ccgatttcga agatggtgtc cgggcgtttg cccagcaggc gatcgccgac
     3301 gaacgtcgcc cccgtcttgt ggcaggtgat ttcgtcgttg atgctgtcgg cgcccaccag
     3361 ttcgccgatc agctcgcggc cgctgccggt gctgccgacg ccccgcacgc ggacgcccca
     3421 gccgacggcc tcctccaatt cgcgcaggca gcgcgtgacg acttcaacgg ggcggccttc
     3481 ggtgcgggtg aagattgaat agatgacgtc gccgcgcgag tcgatcagga ccagcttggt
     3541 gccgaccgag ccgatatcga ggcccaggta ggcgtcaacc ggcgtgcggt cggggaagcg
     3601 gtacgggcga acctggtcgc gaaggagctt gacgcggtcc aggttcagcg ggcgggtggc
     3661 ggcgaacgcg gagccgtcgc cacgtagagc cgtctgcaag gcctccagcc actccgacgg
     3721 gcggcggctg gggcaattcc ggctttccat cgccagcacg gcggcgccaa ttgcgccgta
     3781 tgactccggc gcctcgggca cacacagctc attttcaccg agggcgagtg tttcacgcag
     3841 ggcggccacg accgcggagt tggccgccac gccgccgacg aagaccaccg gcggctcgac
     3901 cgggcggcct ttaaccacgg ccgatttgaa attggtcgcc acggcgttgc atagcccgcg
     3961 cagcacttcg ggcggtttga agcccttctg ctgagcgtgg atcatatcgc tcttggcgaa
     4021 gacgctgcac cggccggcga tctgggccgc gcgttccgcg ccggcaacga tggtcgcgat
     4081 gtcttctaca gcgtattgaa ggcgggcggc ttgctgatcg agaaacgccc cggtgccggc
     4141 ggcgcaatcg ccgttgctac tgtaatcgac gattcccagg cggcggctgc taggatcggg
     4201 caagagccgc agatagcggc tgcactcgcc gcccatctca aagacggtcc gcgcttgcgg
     4261 catgaggcgg tcgacggctc gggcgcaggc ttggaactcg ttgaagaccg gcgctccaag
     4321 ggcttgggcc acgagcttgc cgcctgaacc ggtgatggcg acgcgcaggg cagaatctgc
     4381 aggtaaagcg cccaggacct cttccagagc ttcgcggacg gcttcgagcg ggcggccgcg
     4441 tgtacgcttg ggtgctgcga cgctgatggg cgggactgcg cccggcgtct ggatttggcg
     4501 caaggggtta tggaactcag gttccgaggc gatacgcccg ggctctgagc catccggatt
     4561 ggaagtcgac ccggaagcga gcaaggcgac cttgacgcta atcgcgccga cgtcgatgcc
     4621 gacgtagaaa tccactcgca tgttctcccg cgctgttgac acgcaatagt ttacagcgcc
     4681 attagaggta gcgtgagtgg ccggaaagga caatgacaga taattgccgc ttatctgagt
     4741 ccgaagttgt tgatgcgccg gggcggtccg ggagcgattc cgggaggctg ctgaagcatc
     4801 ccgagcccag ctcgagctga ggaggaaaag gccttttaac accatccggt tgtcggttag
     4861 agcagtgaac gccagtgctc gctcgatcag ccgatttagg acgaagttac tccgatgtat
     4921 gggaatagga tagctgcccc aacgtctata atggtgtcaa cgtaagcgag gaggccgctc
     4981 gtgttcagaa agactcgccc tgcgtttgtg gttgcgacgc tcattctaac gagtttgcca
     5041 gccggcgctt gggcttccga cccggacccg cagccgctgt tcatcatgcg agacgggcgg
     5101 cgggtgcctg tagcggttat tccggcgcgc agcgcgccgg gcaacaactc gcgccaagcg
     5161 gtgctggagg tggttgaggc cgggcaggtg gtgccgcccc aggcgggggt cgcgcggccg
     5221 cgggagcggc ccggggacga gtggggcgga gcgggccgag ccggtggtgc gccaccgcgg
     5281 cggcaggaga tcgcgtatta cgccgggtac ccggtttcgt attactaccc gactgacacg
     5341 tcgtggtggg atacggggcc gatatacccg aacccttaca acacggacta cggcgtcgcg
     5401 gtggagcagc tctatcgcgg gcaacggtat gtctacatgc gtgagcgcgg gcggcgcttc
     5461 aatgaagtgg acatgcagca gcggcgcgag cgtgccttga ccaattccga gacggcgctg
     5521 cggacgggct tggacgattt gaagtcgggc gcatacgagc aggcggtcgt ggcgctgaca
     5581 atggccggtc agttgaatca gggcgatccg gcttcgcgca ttcatttggc gcaggcccag
     5641 atggcgctgg gtcactacga tgaagcggcg gcggcactgc ggcgggcgtt gcaacttcag
     5701 ccgaatttga agttcattcc actgaatctg ggcaagtatt actcggatcc cgaggacttt
     5761 gacctgcacg tgcaacgtct gtacgaatac gtgagggggc acgcagtaac ggcggacgtg
     5821 tacttcctgc tgggctatat gcagttccag cgcgaggact tcgcacaggc gaatgcggcc
     5881 tttcagatcg cactgcgcgg tgcgcccaat gatgccctgg tgcgggaata cgtaaagctg
     5941 acgaagccgg cggcaaggta acccgacgcg cggactgcaa tcctaaactg agcgggggac
     6001 tgcacttgcg acgatgcgat ggggcaggga tgtctccaca ggaataggct tgctattgcg
     6061 cgtctgattt ggtgatattg acgctagcgg tcgctagcgt agcgcccgcc actgtgacag
     6121 catcttctgg aggaagagaa tgacatttcg aagtatgctc gcagtgttgg ggctgcttgg
     6181 ttgcttggtt ggcgaggtgg cacttgcgga cattcaacct ctgccggtcc cattgggctc
     6241 gacggtgcgg ctctccggcg atggctcgac ggttattgcg tcgctcacgg gtggcccgca
     6301 cgggcgttgg actgagacca ccggctttca gccgctgccc ctacccgggg gcgagattcc
     6361 cgccgcttcc cagatgctgg atctgtccta cgacggtcgc accgtgctcg gcttcacggg
     6421 agggagttcg tcgtacctgt ggcgggaagg ggtgggctcg agctgggtgc cctcgacctt
     6481 cggagacagg acgttctatc ccaaggccgt gacgggcgac ggccaaagcc ttatcgggtt
     6541 agcgagcaac ttcgaagacg gcaccatcgt cgctcggtgg ccgggatcgg gcggcctcga
     6601 gtccctttgg gatctcaatc acatcagcct gggcggctac tactcgttac acggggcctc
     6661 gaatacggga gacgcagtcc tgattagcta cccctcaacc ggggagaacc actggttgtt
     6721 cctgcgcgac tccggtgtag ggcctgagct gctgtccgga tcgggaactt acggtattca
     6781 tcgtatagaa gctcggtacg gaccggcttg gtacggaccg gttcggtgcg gaccagctcg
     6841 gtgcggacca gctcggtgcg gacgtcccgc aatcagccgt gcgctcgggc cgggaccgcg
     6901 gcctttgtct ggagcttcga tagcgcctga tccaggtctg cgatgatgtc gtcggggtgc
     6961 tcgccgccta cgcacagacg caccaggttc ggcgggatgt tggccagctt gcggcccgct
     7021 tcgccctgct gctggtgcgt gctgatggcg ggaatagtgg ccacgctctt gatgcggccc
     7081 aagtccgtgg cacgccagat gcgctgcagt ccgtcgaaga atttgcgcgc cgcttcgggg
     7141 ccgcccttca cgcagaagga catcaggtgg ccgtaacggt tcaccggttt gccgtactgt
     7201 tcgtcgtact cggcgtccac cagccacatg tacttgctgg ccagctcgtg tagcggatgc
     7261 tccggcagac ccaggtactg cacgctctca acctgcggat gcttgctgag gaactgggcc
     7321 actttcatcg tgttgcggct gagcaggtcc atcttggagc gcagcgtgcg cacgtcattc
     7381 aaagtcatga tggcctgcat ggggtgcagg ttcgggccgt agtcgcggtt gggcaggtat
     7441 ttgacgtaca gcgcgaagtc cttcttgagg tcttcgttgt cgatgttggt caccaggttc
     7501 ttgcgggcga tcagcgcgcc gcacacgccg aagccgctgg aggtaatcga tttcgtggcc
     7561 gactgcacga cgatgtcggc gccgtggcac agcggccgca gcagcgccgg cgtcgcgacg
     7621 gtcgaatcgc agatcagcgg caggttgtgg ctgtgggcca ggtcggccac ggcggcgatg
     7681 tcaaagaagc tctggcccgg gttgctgggc agctcgccgt acaggaagcg cgtgttctta
     7741 tcgatcttgg aggcccattc gtcgatgttc tccgggtttt ggacccagcg acattcgatg
     7801 tcgcgctctt ccatcaggcg gacgctgaat tgttggaaag ttccgccgta gcactgggcc
     7861 acggccacga agttgcgggc ctcgtgaacg tggcggcgct tgtgcaccag gaagggctgg
     7921 acggcggtca tgatcgcagc catgccggag gaggttgaac agcaggaggt ttcgccgtca
     7981 aagccatagc cttcgagcag ggccagggtc cactcgtaat agtaagtgga ggggttggcg
     8041 atgcgcgagt agcaccaagt ggggatgagg taggccaggg cggcggccat ctcgtcggaa
     8101 tcgcggtagg cctgcgagct gctcatgaag atgggctcga ttatcgcacc ctggtagtcc
     8161 tcgatggcgt cctggacggt gtacaggccg tggacggcga tggtgtcgaa cttccagttt
     8221 ttgaccactt cgcggatgta cttatcgcgc ttggcgaggt aggcgttgtt gaggtcgatg
     8281 taacgctgca ttccgggagt gatctgaacg tcttcgccgc cgaacatcgc tggactcctg
     8341 caaaacgagg gcaaaccaat ccagcccaca gcatacacac tatgtgccag accgccaaca
     8401 aaacgacgag cacggtcccc aggccactga gggcggaaga atgcagggcg attgccccat
     8461 gcaaagagta aaattccgcc cgcggcatcg cgcgcatccc ggcgtaaacg ccgcggctcg
     8521 gcagggcgct gatgcggccg gcgcttggcg ctacagcgaa cggtgcgatg cgcgaacttc
     8581 gatcaggtgg ccaaccgggt cggccagaac gagccattct tcggcggcgc cgaagccgtg
     8641 gtagtgttcg aacggccagc cgcgttcgtt gagccgggcg ataatctggg gcaggtcagg
     8701 cactagcaac gtgagccggc ggcggaccgg atccacgtgg ggatcgtggc gaaattgcaa
     8761 atacagcccg cgggcgggat tgcccagccc ccagccgccg ggaatctgct cggcggccgg
     8821 ccacggacgc aggccgagca agtccgtata aaactcctgc acctccggca gcagctcctg
     8881 cgggatttcg aggcgcacct cacgaacgca gtgcacctcc ggcaattcgg ctgggggttc
     8941 agcggtcggt ttcatccacc tttagcctac ggtccgggcg ggcggaaagc tacggcgggc
     9001 ggagactgca atcggcggaa agctatcgta ggcggaagct gcggtgggtg ggagcgacgt
     9061 ggggcggaaa gcaaaaccag gcggctgacg acggcacgcc tgtaacgagg gcatgcccgc
     9121 gcttacagct tgggcatggc acccgtgcgc ttgcggcnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnngcg gtttcgcgtt tttccacgac tacgtaatcg ccgtcgcgga
     9301 tctggtcgtt gatcatgctg tcgccgcgga cgcgcaggac gaaattgccg cgcgaggaga
     9361 cgaagacttc ctccaagtcg agcgtttcgg gttgttcgag ggcctcaatc ggtttgccgg
     9421 cggcgatcgt ccctaccagc gggagaagcg tggggcgctc gtcggggaac tcgatgccct
     9481 cgctaagtcg caaagagcgt gccctgtgcc gcgaacggcg cagaaaaccc ttcttctgca
     9541 aagcgacgat gtgttcgaag acggttacct tgctgacgtc gaactcgtcc gccaactcct
     9601 gcatggtcgg ggcgaacccg cgagccttcg tgtagtcgcg aatgaacgtt agcacgcgca
     9661 gttgtttggg cgtcagcgat gcttgttgat gcttcaatga cttgacggtc cgcggcatcg
     9721 gtccactcct ctgccaggta ggcgctgaga gagccaagga gaactggcgg cagaggctga
     9781 cgggcggcgg acatgaggcc cagctcgact tccacgtgca gcgcgtccgc cacgggcgcc
     9841 gcggcaggct ccgcgccgcc gaggcagcga gccaggcact gcttgagatc gcgagcctgt
     9901 tccgccgtca ggccgcacga ttcgaactcg ccgcgcgggt cagtccgcac ttcggactga
     9961 aagttcccgc ccgggcccgt gaggcagatc agtcggccgg tcatgttttc gtgcttgctc
    10021 aacactttgg cactccattg ccacatccgt cgccaagcca gcgcgccagt tagagcttta
    10081 ttagcctaat actagcctaa cctgtcaggc gcgtcaagag cagacgcgga aatccgcacg
    10141 aacaacggaa cacaacatcc tgcataataa tagtacaaca ggtcaaccta ttgtgccgtc
    10201 gatgtcggag acaaaatcgc tcccggttcc aaaaaatttc ctgcccactt tgggggtgtg
    10261 aacccgccgg cgcgcgcaaa tgacgcgtat gcaagggttt acggctttcc gaggcgagct
    10321 ggaattctta cgtggtcttg acttgagctc cgccggcgta agcggttccg cgccaattag
    10381 tcgtctttcc cgtcaggcgt gtcatggcgt tgagtgtcat tcccaaacac accgtcgcac
    10441 ccacggggta ggtcaacgcc cagccgggag cgttcccggt cagcgcgtaa aaccgcatca
    10501 ggatggactg ctgcgacaca attgcgaatg ccgccgcagc cgccagccac acgccggagc
    10561 cgccgatcag cggcgacgcc agtaacgcca catacggaga aacgctgaaa atcgatagca
    10621 tgatcacgct cgccagaagg cgcggcaacg tcccgaagca gccgtagaaa atgcgggtcc
    10681 agccgcgcca gatctgcttc aggcccgtgt acatgcgcac gcggtagagg ccggcgctct
    10741 gcagcacctg cagccgcacc cccacgcgct tagcctggcg ggccatgtgc atgtcttcat
    10801 tcagcgtggc cttggcgtac tcgtgaccgc caatcgcttc gtaggcccgc cgcgtcatga
    10861 gcatgaaggc cccgttggcg tacgcctcgc ggcaacgcgg gtcattgacc ttttgcggcg
    10921 gaaaccagaa gaccatgatc gccccggcca ccggctgaat gaccttttcc cacaggctgt
    10981 tcgcctccag ccggggtagc accgacaaaa actcaatccc ctcccgtcgg gcgaatgaca
    11041 cggccgcgcg cagcaacagc ggcgaatcgt aagcacaatc ggcgtctgaa aagcacagca
    11101 actccccggt ggcttgctgc atcgcctcgc gcatcgcatt gttcttacca aaccagccgg
    11161 ccggcaactc gcgcacgtgg acggcggtaa ggcgcggatc gcggcgcgcg tagtcgtcga
    11221 tgatctgcgg cgtgcgatcg cggctgcggt cgtttgcgat gatcacttgc aggcgtggat
    11281 agtcctgtgc cagcaagccg tcgatgcacc gcgcgatgtt ctgctcctcg tccttggccg
    11341 ctaccacgac tgacaagctc ggccagccgt tgctgtctgc gccgccgccg gattggtcga
    11401 ctgtttgggc gtcctgttct gacaaaacgt gctctttccc gccgcgattc acctgcaggt
    11461 ggcggcggag ccagacgagg ccgatggcga gcgtgatcca gaaccaggcg gcgatccagg
    11521 gcgaggcgaa atcaggcatg gagaactcag gctttctgcc gccgcgcggg tctggcgcgg
    11581 acggttgcaa aacaaaaagg cagcgactcg tgcgcgccgc cggttgtcta ctttaacggc
    11641 gctgccgctg gacgaaaact ggcggttgga actccgaagt ctccaatctg tttgccgcac
    11701 cgtaggacgt gcctttggat caaccgcgca agtgctcctc aagccggtcg agcagctcga
    11761 cttgagcggg gttgatcttc tgtcgcgctt cgtccggcgt gaagaaggcc gcgcggtcga
    11821 tttcggggaa tgtacgagtt tggcccgagc gtggcggcca ttccattgtg aaagtgttgc
    11881 tgcgcagggc agcggggtcg cagtcggatt cgaagaccca cgcgtggatc agcttgccgc
    11941 ctttttgcct gattgtgccg agcgggatga atgggccggc cggtttgagg ccggtttcct
    12001 cctcgaattc gcgaatcgcg gcggccagcg aatcttcgcc ggcggcgatc tcgcctttgg
    12061 gaatcgtcca cgcgccggcg tctttcttgg cccagtacgg cccgccgggg tgaacaagga
    12121 gaacctgcgg ctgtccatgg cggatgcgga acatcagcaa tccagcgctg ggcgtaggca
    12181 tacggggaat tgtaagcgga gcgggggcgt gtcgccccgg agtgcggggt ggttggacgc
    12241 caaagtgggg cggcgccgtt gcttccgggg gcctttggtt ctccggcccg gcacgggccg
    12301 gctttgcttg tggcgcgcgg cgcaggctgc ttatgatggc gccctgagcc cgggcgagcc
    12361 tatgggcggc taaccgccgc ctccgaaacg tgtccaaagc ctggtaacaa cgttttgact
    12421 tttgctatcg agggagcacg atgcgtaccg ctacgatctt gatcgccatg acggggctct
    12481 tggcctgccg ttcggccggc gcccaggaca ttaccgtcga gcagcagaaa ctgctggcca
    12541 ggcgggcggc cgaagcggac gcctaccgga aactcgcgca aatcgtttac gggattcaac
    12601 ttaacgaacg gacgtacgtg caggatttcg tctgtgaatc ggacgagatt cgtggcgagg
    12661 tggatacctt cataaagggc gtccggctgg ggcagccgat cttctacgac gacggctcct
    12721 gcgaaatccc ggcggaggtg accgtcgcga aggtggttga aacgctgcgt acggcgtgcg
    12781 accgctggta caagggggag gacatcaaga ccagcgattt cgagtcgatc agccgccgaa
    12841 ttcagaaaga cgtgatcaag gtctgcggca tgggagcccc gcgggcggac ttgccgccgg
    12901 atctgccgca atgcgcgatg gaaatgctgc cgccgggtcc gccgatcgcc gaaaatgcgc
    12961 cgccgccgcc gattccggat atctggaagc agatcgggcc gcaggcgcgc ctttcggcca
    13021 tgagggccgc gcagatggac gccatgcgga agctggccga acgcatcaag ggcctgcgcc
    13081 tcacctcgca gacctgcgtg cgcgacttcg tagcggaaag tgaccgaatt acggctgaca
    13141 tgcaggcctt tttgaaaggc gcccgcgaga tatgctgcta cttccacagc gacgagctaa
    13201 tctgtgaatg cacgtacgtc gtgccgaccg agcaggtcat cacgaccatc aagcaactcc
    13261 acagccgcta ttacaacggc gacccggacg atacgcgcgg ctcggatatc gagaagatcg
    13321 tcaagacggt cgtgaagaag gacttcgaag cgacgggtat gggcgtcccg aacccgcgct
    13381 tcatgcagaa gttccaggaa tcggcggccg tccgcatgcc cgactggtcc atgcgcacgc
    13441 tcgaagctga cgggcgcggg acggacccgc aaatagacac gccgcaaggc cggctgcgtg
    13501 cggcgcgggc cgccgaaatg gacgcgcggc gccgactggc cgagcagatc gccggcttgc
    13561 aattggaaag cgcgacgacc gtgagcgact ttgcgatgca gcacgatgaa gtgcgcggcc
    13621 tggtagaggc ggtggtcgtg gacgcagccg tgaccaacac gcgcttcgat caggacgtcg
    13681 cggtcgtgac cgtggccctg ccgggcatga gcgtctggag cgtcgtcaac gacgggctcc
    13741 cgcgaaacca gtagcggccg cgcgccaggt aagtgacagg agaaaccgat gcgtcttttc
    13801 gtttcgctcg cgatttcgtt ggctttgatg ttgacggtgg gctgcgcttc gcagcgtcac
    13861 cacaaggtgc gcgtgctgga agaagagcgc gtgggcgaag tgcaggaagc gccgcagggt
    13921 gagatgatcg tcgaatagcc tgggctgccg ccatgcacgt gtggcctcag tggttgttgt
    13981 gctcgtcgcc ttgggcgtgc gtgctgccgg ttgcaatcgg ttgggcggcg ggagcgctga
    14041 gcctgcaggg ccgcgcgttg gaacgagtcg cgctcgccaa agcgcgcagc gaactcctcg
    14101 accgggtcta tgctctgaag ctgcgtgagt cgcttaccat cggcgattgg gcggcgcacg
    14161 atctgggccg cgaccgggcg ctgcgcctgt ggctacgcca gacggcagcg tacggagccg
    14221 cccgtactta ttcagacggc tcgtgcgacg tggcggtgcg gctcgccccg gcagcgattg
    14281 agcagaagct cgaggcatta tccgcgacca gcggcgccac ggccgacggt gcggtcgcga
    14341 ccccggactt ttcacttgcc gcgtgcgaat ggccggttct ttgggcgcaa ggtacggcct
    14401 ggcccactga ggcggagctg gcgcagcaac cggaaggctg ggaagacgtg acgcctgtgg
    14461 gcttggagat gacacgtcgg gcggccgagg cggacgcgcg gcgggcgctg tttgattcga
    14521 ttgggatgtt gccggtaacg cgagcgcggc ggctgagcga ttttctcgaa tcgggcgagc
    14581 aggtgcgtga agctgttatt gccgcgttgc agcgcgaggc gctgctggca gtcagcttcg
    14641 cgccggacca ggtggccgtc gccacggcgc gcatcgggct ggttgatttg atccgcattc
    14701 tcaccgaagt tcacgagaac aactatcagg gcgatgggtt tcacggccgc gacttccgcg
    14761 gaatgggact gctggcgacg gtagatgagg tgagtgcgac cggctgggcc attccgcccg
    14821 cggccgcggt ttcgcgcccg cactaccct
//
LOCUS       DAOSYS010000065        14665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3454_length_14665_cov_5.351951, whole genome shotgun sequence.
ACCESSION   DAOSYS010000065 DAOSYS010000000
VERSION     DAOSYS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14665
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3454_length_14665_cov_5.351951"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PLU99_05710"
     CDS             <1..1829
                     /locus_tag="PLU99_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOO16589.1"
                     /translation="RARGLTAAIVHAGGILPQNQIGPERWQAALDLIYDRSRDGVTPL
                     ERFISLFADGEQLAQTVSISALPLEERIKQRIIEGNRLGMEDDLNQALQRFPAIDIIN
                     NLLLGGMKTVGELFGSGQMQLPFVLKSAEAMKAAVSHLEPHLEKSETAQRGSIVLATV
                     RGDVHDIGKNLVDIILSNNGYTVHNLGIKQPINQVIEAWQKHRANAIGLSGLLVKSTL
                     VMRDDLEVLNEHGITVPVILGGAALNRRYVEDELRKIYRGPLYYARDAFEGLTIMREL
                     TEPAGREKPSGAGEESEGDGDAGDDRPVPPLVSAKFGCRTGQAAPGAKADTAPLEYTC
                     RVPQPPFWGDRVLTGIPLAAVLPYVNEVALFQARWQFRRHRRPAADFAQYLDEVVRPI
                     YRELIARCEREDIIQPRVVYGYWPCNSAGNDLIVYAPPPQADAAPREHGPELLRFSFP
                     RQREAPYRCLADYWRPASDGVTDVVAFTLVTAGPRASEAAAELFSAGHYQDYLFLHGL
                     GVEVAEALAEYAHRQVRLELGVADHDAQDVRALFRKSYQGCRYSFGYPACPALEDQEL
                     LMKLLRPERIGVTLSERFQLEPEQSTSAVISYHPEARYFNVR"
     gene            2015..3232
                     /locus_tag="PLU99_05715"
     CDS             2015..3232
                     /locus_tag="PLU99_05715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO16590.1"
                     /translation="MALFRQPLKSASRVGRISSSLQGQVKNVGKALRGLRPTARSTAW
                     LRLRRKPTAAERVQDVGKRLLSSPTFRVLAAAGAAYGVYKLVQKLREESLRGQVVLIT
                     GGSRGLGFLLAREFAREGCRVAICARDEDELADAARELRRQGADVCALRCDVAERRQV
                     LSLIAEVTARFGQIDILVNNAGVMHVMPLAAATEADFERALDIMFWGTLHASLAAARH
                     MRPRRTGRIVNITSIGGFVSVPHMAPYSCAKFAAVGLSQALHAELAPDGISVTTIVPG
                     LMRVGSHLNAWFRGEQKKEFAWFSTAASVPLLTISAERAARQIVAATKRRESLRILGL
                     PANILERLHGLFPGLTNRIMALVNYSLPAMSRTGTTSDARGIDLEPELRSRLRELITK
                     PGRVAAQYTHETR"
     gene            complement(3259..4272)
                     /locus_tag="PLU99_05720"
     CDS             complement(3259..4272)
                     /locus_tag="PLU99_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOO16591.1"
                     /translation="MPSGRSTAARLALLAVIAVVIVASSSLLGPTPLSEIFGQRAPNL
                     FWQLRVPRTCLAAAAGAGLALGGVIFQALFRNPLAEPYTLGVASGASLGAAVAMLTGL
                     TGFLGWIPLSVLLALAGAAGALGLVYLMARLRGGQSMTHLLLAGVCVSYLSAAGILLV
                     TYASDGTVTNEIVVWLMGSLSFYRPAASLEVVALVLVVLAFAIFASRGLDLLALGEHV
                     AASRGVAVQPLIWTSFVLVGLLTAVIVANCGPIAFVGLMIPHFGRALFGARTLPLTIA
                     AALLGAAFLAACDGLGRVALSLIHQRPVGYEFPVGVLTSIVGSAFFFYLLARAEPARI
                     GPE"
     gene            complement(4274..4939)
                     /locus_tag="PLU99_05725"
     CDS             complement(4274..4939)
                     /locus_tag="PLU99_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16592.1"
                     /translation="MIRPGFGSWSFLLSLVFIAASQTGCVGLRGLAYLMQPRQVQKPE
                     VDLSGRVAIVIDYARANHANPVFETTFHKRVVELLRENKVKAEILAYDEIDRLRQSHS
                     DFASWSVQKIGRELNADQVLYVRIEELRLAQKNNPLLIPSVALRLKLIAPRAPAANAR
                     LWPDADRERDGREVTCKRQPGENEGAERVDSETAKLARETAYYVARNFYEYDLEIQPP
                     REP"
     gene            complement(4936..5586)
                     /locus_tag="PLU99_05730"
     CDS             complement(4936..5586)
                     /locus_tag="PLU99_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16593.1"
                     /translation="MSRIVRRSGWPRHVALLGLLLPMLGGCKLIALPFFAFGQDQTKK
                     VPAEYPYLEGKKVAILVWVDEYTSFQYQFLPLEVSEHVRAALEGSVRGVSVVPNRQII
                     DYQKREPNWQSRNPAEIGKEFGADRVIVIEVTQYTTREPGSTHLYRGHMGGNVKVLDT
                     AYPQSGPAYQTVIEVAYPPDRIAAYGTDERTLRRAIMELFANDLASRFHEHREKVK"
     gene            5937..6689
                     /locus_tag="PLU99_05735"
     CDS             5937..6689
                     /locus_tag="PLU99_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16594.1"
                     /translation="MAEAQTEQFTIVCRDCHRPMMARRVWEGRDVQCPYCSGVARVPP
                     PPTGHLAVPAGPPAMGPRLEFNFACPRCETLLESHTGLTGQPGRCPACGAKILVPEVN
                     RRTGRPQRSQLLDDGGEAPAPIHAYAASGLQAPRIFRRADGTQVIECPRCSETCPVDS
                     DSCPACHTPFTAEVVPSIRSYKSNRRAQIALFLGIASLPLCMLFVPALAAVVFGTLSL
                     GLGSGSRPPVAGIVGITLAALSLAGGLLLFLL"
     gene            complement(6827..7375)
                     /locus_tag="PLU99_05740"
     CDS             complement(6827..7375)
                     /locus_tag="PLU99_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOO16595.1"
                     /translation="MHKATAWIVGLGVLMVAVAAYPVIAQSQDQPGQRQPGQRPQPGQ
                     PGQPGQPGQYGQPGQRGQADMAKPLVSANDFKQMEQQQTIPLLQAIQSVEAQGKGKAV
                     AVIPVREQNEFRFNVYTMKDGQLTLHEIDAKTGRETNQRPTNAIAELGMSRFVGHEEM
                     GGQQQQQPMPPRGGDRPSPIRP"
     gene            complement(7547..9058)
                     /locus_tag="PLU99_05745"
     CDS             complement(7547..9058)
                     /locus_tag="PLU99_05745"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16596.1"
                     /translation="MPSESSVELLMPQSLAWALRYLPDYFSDQPARFHCELMSDLENP
                     ANRLLARIAPRGHAKSTCAALAYPLWCLCERRRRNILIITQESSLATQFVRDIRAELE
                     SNEAILAEYGDMCRQPDCAPVSKKPARAARRKRGGKSPVPRRRGKWSESKFITACGLA
                     VQGKGCGTSLRGTRVGPQRPDLIICDDIEKDDLVRSPQARRNLEYWLRRVVMPALAPE
                     GQLVVVGSLIHFDCLLANLSDEKRFPHWNYRIYKALEEKRGRDGKIHRIALWPARWPV
                     EKLDEERDRIGVPAFEQEYMANPIDDSRPTFKPEWLRRYDPAELENCEERLINLMAVD
                     PATGSSSGDFCAYWVGSVDQANGIIYTRELLLERTDINGQVQRIMELYQRWRPLRVGI
                     ETVGYQVALKHNVERESAKVNAFVPVVSLKTTFNKTARIQGTSPLYQSGKFRLPPVLD
                     PEAELQFLQFPHGAHDDAPDVCAMGIELAREFLGRHTVEVVSRRGSGLDRRKW"
     gene            9307..10542
                     /locus_tag="PLU99_05750"
     CDS             9307..10542
                     /locus_tag="PLU99_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO16597.1"
                     /translation="MDGFARDHARGAVRSLPRAARVAAGALALLTALFPACRRQGASQ
                     NAEAARALPAPITVATAEIADVPIAIGVVGRAESVNTVTITPRVSGQVDDVHFMDGQY
                     VEAGTLLYTIDQRPFQAAVDQAKATLARDKALAAEAAKKAQQYEQAYRTKAAAEVDRD
                     TAVANAAATAATVEGDEASLENALLQLEWCTIRSPIAGKVGYRLVDVGNIVTANQTAL
                     VVINQIEPIYVTFAIPERYLAEVHARLNSGPPEVEATIPGDSGVVRRGELTFVDNQVN
                     PATGAITLKGTFANTDRALWPGQFVNVNLILGVQARVVIPSEAIQSAQQGEFVYVLTP
                     DDTVELRTVVTGRTLEGRTVIMEGLKAGEVVVKEGQTRLQPGAKVSVQPAASRPTNAN
                     SSAPKKTGVTRTRDNGLQP"
     gene            10579..13692
                     /locus_tag="PLU99_05755"
     CDS             10579..13692
                     /locus_tag="PLU99_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HOO16598.1"
                     /translation="MFSRFFIERPILANVIAVITMLLGGVALLVLPAELYPQITPPTV
                     RVSANYPGGSAKVVADTVGAPIEQQVNGVENMLYMSSTSSNDGSYALTITFEVGTNID
                     MAQVLVQNRVQIALAQLPPEVQRQGITTLKQSTDIVMFVTLTSPDERYDSLFLSNYAT
                     IHIRDALSRIPGVGQVTVIGAGSYSMRLWLDADELAARGLTTLDVVNAVEEQNVQVAA
                     GQIGQPPAPPDQATQLTVTTLGRLRTPEQFGDIIVKTITAPTPQIVRVRDVARVELGA
                     QTYDQFFQVNGVPAAGMAVFQLPGANALDVADRIRAEMQQLREQFPPGLRYDIPFDTT
                     LFVRAAIRELYFTLLEAGVLVLLVIMVFLQDWRAVLVPATTVPVTLVGAFIALFAFGF
                     SVNLLTLFGLVLAVGIVVDDAIVVTENAAHHIESGQQPKEAAIAAMVELFGPIIGITL
                     VLTSVFLPAAFLRGITGQLYRQFALTIAATAVISAINALTLKPAQCALWLRPAVQRPR
                     FIFFRAFNSFYGRVEHAYARSVHWMTRRSALFMIPYALLVAVTLWGFLALPTGFLPIE
                     DQGYFMTSVQLPDAASQDRTKAVVDRLTDILHSTPGTKNVNALIGQNIFEQTVASNAA
                     AIYTTLQPWDERGPAESQEAILEHVSKMYRALPQATVISFPPPAIRGLGVTGGFQMAV
                     QDRGDVGLAELGQAARALIMAANATPELRGVNTTFSDRVPQIYLNIDRSKVKSLGVPL
                     SSVFNTLQTYLGSNYVNDFNLFGRTYQVRLQALPHFRDKIADVLGLYVRNESGEVVQL
                     GTLLNVEHRAGPQTLTRYNLYPAASITGQAAPHVSSGQALAIMERLADEHLPRGVGFE
                     WTATSYQEKRVGRQALIVFALAVSLVYLVLAGQYESWSDPAAVILVVPLALLGAVVAV
                     ALRDMPNSIYTQVGIVLVIALASKNAILIVQFAREQRRRGMSIADAAAEAARLRLRPI
                     LMTSFAFIFGVVPLVIATGAGAASRRALGTAVFGGMIASTLLAVFLVPVFFVVCERLS
                     ERLRRKV"
     gene            13734..14339
                     /locus_tag="PLU99_05760"
     CDS             13734..14339
                     /locus_tag="PLU99_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO16599.1"
                     /translation="MSEDSGHECGVEREYDKLADEALMALVAQGRREAFTVLVGRHQA
                     GVMGVAYRFMGQRDVAEDVAQDVFLKVWRSASAFRPEAQFTTWLYRLTANLCWDRRRR
                     AAREARFRIAFLQTRTASEPPSNLDSHERVELVRRAVARLPDRQRLAVILHRYAGLSH
                     REIAEVTGWSVKAVESCLVRGYGALRKSLAQLDSRRANQAR"
     gene            14427..>14665
                     /locus_tag="PLU99_05765"
     CDS             14427..>14665
                     /locus_tag="PLU99_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOO16600.1"
                     /translation="MKHARRLDLIRLAADELPEQDRAEVERHLATCPACRSLYEQQAA
                     TWRALGEWAPETAHVDLLAQIDRKLSERPATRPPF"
BASE COUNT         2678 a         4527 c         4728 g         2732 t
ORIGIN      
        1 ccagggcgcg cgggctgacg gcggccatcg tgcacgccgg tggaattctg ccgcagaatc
       61 agattgggcc ggagcgttgg caggccgcgt tggacttgat ctacgaccgc tcgcgggatg
      121 gcgtcacgcc gctggaacgc ttcatcagtc tcttcgccga tggcgagcaa ctcgctcaga
      181 cggtcagtat ctccgcgttg ccgctggaag agcgcatcaa gcaaaggatc atcgaaggca
      241 accggctggg aatggaggac gacctgaacc aggcgctgca gcgctttccg gccatcgaca
      301 tcatcaacaa cttgctgctg ggaggcatga agaccgtcgg cgagctgttc ggcagcggcc
      361 agatgcagtt gcccttcgta ctcaagagcg cggaggcgat gaaggcggcc gtcagtcacc
      421 tcgagcccca cttggaaaaa tctgagactg cgcagcgcgg cagcatcgtt ctggccacgg
      481 tgcgtggcga cgtgcacgac atcggcaaga acctggtcga tatcattctc agcaacaacg
      541 gctacacggt ccacaatctc ggcatcaagc agcccatcaa ccaggtcatc gaagcctggc
      601 agaagcaccg tgccaatgcg atcggcctga gcgggctgct ggtgaagtcc acgctcgtta
      661 tgcgcgacga tctggaagtc ctgaacgagc acggtatcac cgttccggtg atcctgggtg
      721 gggcagctct gaatcgacgc tatgtggagg acgagctgcg caagatatat cgcgggccgc
      781 tttattacgc ccgcgacgcg ttcgaaggcc tgacgatcat gcgcgaattg actgagccgg
      841 cgggccgcga gaagccgtca ggcgcgggcg aagaatcgga gggcgacggc gatgccgggg
      901 acgaccgccc tgttcctccg ttggtctcgg ccaagttcgg atgcagaacg gggcaggctg
      961 cgccgggggc caaggccgac actgcgccgc tcgaatacac gtgccgcgtt cctcaaccgc
     1021 cgttctgggg tgaccgcgtg ttgaccggca ttcctctggc ggccgttctg ccctacgtca
     1081 atgaggtagc gctttttcag gctcggtggc aattccgccg acatcgccgc cccgccgcgg
     1141 acttcgcgca atacttagac gaagtggtgc ggccgatcta ccgtgaactc attgcgcgct
     1201 gcgagcgtga ggacattatc cagccgcggg tggtctacgg atattggccg tgcaattcgg
     1261 cgggaaacga cttgattgtc tacgcgccgc cgccgcaggc tgatgctgcg ccgcgcgagc
     1321 atggcccgga actgctgcgc ttcagctttc cgcggcagcg tgaggcgccg taccgctgcc
     1381 tggccgacta ttggcgtccg gccagcgacg gcgtcaccga cgtggtcgcg ttcactctgg
     1441 ttactgctgg gccgcgcgcc tcggaagccg ctgcggagct gtttagcgcg ggacactatc
     1501 aggattacct gttcttgcat ggtctggggg tcgaagtggc cgaggccctg gcggagtatg
     1561 cgcatcggca agtgcgactt gaactgggag tagccgatca cgacgcgcag gacgtccgcg
     1621 ccctgtttcg caaaagctac cagggctgtc gctacagctt cggctatccg gcctgcccgg
     1681 ccctggagga tcaggaattg ctcatgaagc tgctgcggcc ggagcgcatc ggcgtgactc
     1741 tttccgagcg atttcaacta gagcctgaac aatccaccag cgctgtgatt tcttatcacc
     1801 cggaggcgcg ctacttcaac gtgcgttgat cacgcccgtt cctgtacttc gcgagccgtg
     1861 atcttggcgt tgagatttca ccctccggac taattcgccc ctccggacaa attcgccgcc
     1921 tatctccaaa ttcgccgctc tgtcatacgg cgttaagttc gccgggccac cttttggcgt
     1981 tagcgcgttt tgtgtttaag cgaggagtgg caatatggct ctgttcaggc aaccgctcaa
     2041 gtcagctagc cgggtcggcc gaatctccag ctcgctccaa ggccaggtta agaatgtggg
     2101 caaggcgcta cgcggcctgc ggcccaccgc gcggtcaacg gcgtggttgc gtctgcggcg
     2161 gaagccgact gcggccgagc gggtgcagga cgtgggaaag cgtctgctgt cttcgccgac
     2221 gtttcgggtt ttggcggcgg cgggcgcggc ttacggcgta tacaagctgg ttcagaagct
     2281 gcgcgaggaa agcctgcggg gccaggtggt gctgattacc gggggatcgc gcgggctggg
     2341 gttcttgctg gcgcgcgagt ttgcccgcga aggctgccgg gttgcgatct gtgcccggga
     2401 tgaagacgaa ctggccgacg ccgcgcggga actgcggcgg cagggcgccg atgtgtgcgc
     2461 cctgcgctgc gacgtggccg agcggcgcca ggtactgagc ctgattgcgg aagtaacggc
     2521 gcgcttcggt cagattgaca tcctggtgaa caacgccggc gtgatgcacg tcatgccgct
     2581 ggcagccgcg accgaggccg acttcgagcg ggcgctcgac atcatgttct ggggcacgct
     2641 tcatgcgtcg ttagcggcgg cgcggcacat gcgtccgcgg cgcaccgggc gcatagtgaa
     2701 catcacctcg atcggcggct ttgtgagcgt gccgcacatg gcgccgtata gctgcgccaa
     2761 gttcgccgcg gtcgggctgt cgcaagctct gcacgccgaa ttagcccccg acggcatttc
     2821 ggtgacgacg atagttccgg gcctgatgcg cgtcggctcg cacctgaacg cctggtttcg
     2881 cggcgaacag aagaaggaat tcgcatggtt ttcgaccgcg gccagtgtgc ccctgttgac
     2941 cataagcgcg gaacgcgccg cgcgacagat cgtggccgcc accaaacgcc gcgagtcctt
     3001 gcgcatactc gggttaccgg ccaatatcct ggagcgcttg catggcctgt tccccggatt
     3061 gacaaaccgc attatggcct tggtgaatta cagtctcccc gccatgtcgc gcaccggcac
     3121 gaccagcgat gcacgcggca ttgatcttga gccggaactg cgctcgcgtc tgcgtgagct
     3181 cattacgaaa ccggggcgtg tggcggcgca gtacacgcat gaaacgcggt agcgaccggc
     3241 ggctaaagcc tttccggttc attccggccc gatacgggcc ggctctgctc gcgctaggag
     3301 atagaaaaag aacgccgacc cgacgatgct cgtcaatact ccaactggaa actcgtagcc
     3361 gaccgggcgc tgatgaatga gggatagcgc cacccgcccc aagccgtcgc aggccgccag
     3421 aaacgccgct cccaaaagcg cggctgcgat tgtcagcggc agcgtccgcg ccccgaacag
     3481 agcccgcccg aagtgcggaa tcatcagccc gacaaaggcg atcgggccgc aattcgccac
     3541 gattaccgcc gtcagcaggc ccaccaacac aaaagacgtc catatcagcg gttgcaccgc
     3601 aacgccgcgt gatgcggcca cgtgctcgcc gagcgccagc aaatccaggc cacggctggc
     3661 gaagatggca aacgccagca caaccagcac cagggcaacc acttcgaggc tcgcggccgg
     3721 gcggtagaag ctgagcgagc ccatcagcca cactacgatt tcgttcgtga ccgtgccgtc
     3781 ggatgcgtaa gtcacaagca ggattcccgc cgcgctcaag taagaaacgc acactcccgc
     3841 cagcagcaga tgcgtcatac tctggccgcc gcgaagccgg gccatcagat agaccagtcc
     3901 cagcgccccc gccgcgccgg cgagcgccag cagtacgctg agcggtatcc agcccaggaa
     3961 gccggtcagc ccggtgagca tggcgacggc cgccccgagc gatgctccac ttgccacccc
     4021 gagcgtgtag ggctccgcca atggattccg gaagagcgcc tggaagatta ccccgcccag
     4081 cgccaagccc gcgccggccg ccgccgccag gcacgttcgc ggcacgcgca actgccagaa
     4141 caggttcggg gcgcgctgcc cgaaaatctc gctcaaggga gtcgggccca gcaagctgga
     4201 actcgcgacg atcacgaccg cgataaccgc cagcagtgcc agacgggccg cggtggaccg
     4261 cccgctgggc acgtcagggt tcgcgcgggg gctgaatctc gaggtcgtac tcgtaaaaat
     4321 tccgggcgac ataataggcg gtttcgcggg ccaacttggc ggtctcggaa tcgacccgct
     4381 ccgccccctc attctcgcca ggctggcgct tacaggtgac ttcgcggccg tcgcgctctc
     4441 tgtcagcatc cggccaaagc cgggcattcg cggccggagc gcgcggcgcg atcagcttca
     4501 gccgcagggc gaccgaaggt atcagcaagg ggttgttctt ctgcgccagc cgcagttctt
     4561 caatgcggac gtagagcact tggtcggcgt tcagctcgcg gccgatcttc tgcacgctcc
     4621 aggaagcgaa gtcggagtga gactggcgca agcggtcgat ctcgtcgtaa gccaggattt
     4681 cggccttcac cttgttttcg cgaaggagct ccactacgcg tttatggaac gtggtttcga
     4741 aaactggatt ggcgtggttg gcccgggcgt agtcgatgac gatcgctacc cgcccactga
     4801 ggtcgacttc cggcttttga acctggcggg gctgcatcag ataggccagg ccgcgcagcc
     4861 cgacgcatcc cgtctgagac gcagcaatga acaccagcga caggagaaaa gaccagctac
     4921 caaagcctgg gcgaatcact tcaccttctc ccggtgttcg tgaaagcggc tcgccaggtc
     4981 attggcgaac agttccatta tggcccggcg cagcgtgcgt tcgtccgtac cgtaggcggc
     5041 aatgcggtcc ggtggatagg cgacttcaat cacagtctga taggctgggc cggactgcgg
     5101 gtacgccgtg tcaagcacct tcacattgcc ccccatgtgg ccgcggtaca ggtgcgtact
     5161 gccgggctcg cgcgtagtgt actgcgtcac ctcgatcacg attacgcggt cggcgccgaa
     5221 ttctttgccg atttcggcgg ggttccgcga ctgccagttc ggttcgcgct tttgataatc
     5281 gatgatctgg cggttcggaa ccacgctcac cccgcggacg ctgccttcca aggccgcgcg
     5341 gacgtgttcc gaaacctcga gcggcaggaa ctggtactga aagctggtgt actcgtcgac
     5401 ccagaccagg atggccacct tcttgccttc caggtacggg tactcagccg ggactttctt
     5461 ggtctggtcc tgcccgaagg cgaagaacgg cagggcgata agcttgcagc cgcccagcat
     5521 gggcagaagc aatccgagca aagcgacatg gcgcggccag ccagaccgcc gaacgattct
     5581 cgacacttcg taacctccaa gactcgcctg aggctgcgtg cgcggagcaa gcggccggcc
     5641 acattacaac cagccgcgcg ccttgatccg aagcgctagc gaggggccaa agctcgccga
     5701 ctctcggccc tgcatggcgc gctgcgggat cacgcacctg atttgacacg ataaccgtca
     5761 ggcggcggtt tgggtagcgg gccggtcatc ttacagtcgc cccctcatgg ccgaattggt
     5821 cctgattcgc gagccgcgct cgcgagcgtc tcggcccccg gtttttgccc tggctggcgc
     5881 ttctggctcg gatcaagggg cgcgccacgc ccgaatttgc ttacaattct cgcgtcatgg
     5941 ccgaagctca gacggagcaa ttcacgatcg tgtgccggga ctgccaccgg ccgatgatgg
     6001 cccgccgcgt ctgggaaggc cgcgatgtgc agtgcccgta ttgttcaggc gtggcgcgcg
     6061 ttccgccacc gccaactgga cacctggccg tgccggcagg cccgccggcg atggggcccc
     6121 ggctcgagtt caacttcgcc tgcccgcgct gcgagacgct gttggagtcg cacaccggcc
     6181 tgaccggcca gccgggccgc tgcccggctt gcggcgcaaa gattctcgta ccggaggtga
     6241 accggcgcac cggccggccg cagcgttcgc aattgctgga tgacggcggc gaggccccgg
     6301 ctccgattca cgcctacgcc gccagcggcc ttcaggcgcc gcgcatcttt cgacgcgctg
     6361 acggcacgca agtcatcgaa tgcccgcgct gttccgaaac atgcccggtg gacagcgata
     6421 gctgcccggc gtgccacacg ccgttcacgg ccgaggtggt gccatcgatc cgcagctaca
     6481 agtcgaatcg ccgggcccag attgcactct ttttgggaat tgcttctctg ccgttgtgca
     6541 tgttgttcgt gcccgcgctg gccgcggtgg tatttggcac gttgtcattg gggctgggct
     6601 cgggtagccg gccaccggtc gcgggaattg tcgggataac gcttgccgca ttgtcgttgg
     6661 ccggcggtct gttactgttt ctgctctaag tcaccgcatg ccccgccgaa actcagagca
     6721 aagctggcgc gagtgcatcg tttgagagca ccgggcgagc cattcccgtt cggcccgccc
     6781 ggatgtaaag cctcaacgca gctctaactt acccagacac cggaccttat gggcgaattg
     6841 gactcggacg gtcgccaccg cgtggcggca tgggttgctg ctgctgctgg ccgcccatct
     6901 cctcgtgacc aacgaatcgc gacatgccca actcggcgat cgcattggtc ggtcgctggt
     6961 tcgtctcgcg gccggtcttc gcgtcgattt cgtgcaacgt gagctgcccg tccttcatcg
     7021 tatagacgtt gaagcggaac tcattctgtt cacgaacggg gatcaccgcg acggccttgc
     7081 ccttaccctg cgcctcgact gactgaatcg cctgtaagag cggaatcgtc tgctgctgct
     7141 ccatctgctt gaagtcgttg gcactgacca gcggcttggc catgtccgcc tgcccgcgct
     7201 ggcccggctg gccatactgg cccggctgac cgggctgacc cggctgaccc ggctgcgggc
     7261 gttggcccgg ctggcgctgg ccgggttggt cctgactctg agctatcacc gggtaagccg
     7321 ccaccgccac catcaatacc cctaacccca caatccacgc agttgccttg tgcattgata
     7381 tctcctgaga ttgtcgcacg ctcttcgctg cacggctaca caccgcccgc ctcgaggtgc
     7441 ccgttgcggg aactgtctgg tgatagtcgg gcgcggggtg cccggcaact aaaaacggct
     7501 tcagagcagc ggcgcagccc aattcggctg ttgggcggcc ggcgagttac catttccgac
     7561 gatccaaacc gctcccgcgg cgactgacca cttcaaccgt atgtctgccc agaaattcac
     7621 gggccagttc gatccccatg gcgcatacgt caggcgcgtc atcgtgggcg ccatgcggga
     7681 actgtagaaa ctgcaattcg gcctcgggat cgagcacggg cggaagtctg aactttccgc
     7741 tctgatatag cggcgaagta ccctgaattc gcgctgtttt gttaaaagta gttttgaggc
     7801 taacaaccgg tacgaaggca tttaccttgg cgctttctct ctccacgttg tgcttgagcg
     7861 caacctgata acccaccgtc tcgatgccga cgcgcagcgg gcgccagcgc tgatagagtt
     7921 ccatgatccg ctgcacctgg ccattgatgt cggtccgctc cagcagcagc tcacgcgtgt
     7981 agataatccc gtttgcctga tcgacactgc cgacccagta cgcacagaaa tctccggacg
     8041 aactcccggt ggccgggtcg actgccatga ggttgattaa tcgctcttcg cagttttcga
     8101 gctcagccgg gtcataccgc cgcaaccatt cgggcttgaa cgtcgggcgt gagtcgtcaa
     8161 ttggattggc catgtactcc tgttcaaagg ccggcacgcc gatgcggtcc cgctcctcgt
     8221 ccagtttttc caccggccaa cgggcgggcc acaacgctat gcggtgaatt ttgccgtccc
     8281 gcccgcgctt ctcttccagt gctttgtaaa tacggtagtt ccagtgcggg aagcgcttct
     8341 catcgctcaa attcgccagc aggcagtcaa aatgaatcaa agagccgacg acgaccagtt
     8401 gaccctccgg cgccagggcc ggcataacca cgcgccgcag ccagtattcc aaattgcgcc
     8461 gcgcttgagg cgatcggacc agatcgtctt tctcgatatc gtcgcaaata atcaggtccg
     8521 ggcgctgcgg tccgacgcgc gtgccgcgca agctggtgcc gcagcctttg ccctgcaccg
     8581 ccaggccgca ggccgttatg aatttgctct cggaccattt gccgcggcgc cgcgggacgg
     8641 gtgacttgcc gccgcgcttg cggcgcgccg ctcgcgccgg ctttttggat accggcgcgc
     8701 agtccggctg gcgacacatg tcgccgtatt cggccaggat ggcctcgttg ctctccaact
     8761 cggcacgaat gtcacggacg aactgggtcg cgagcgagga ctcctgcgta attatgagga
     8821 tgttgcggcg gcgccgttcg cacaggcacc ataacggata ggccagggcg gcgcaggtgc
     8881 tcttggcgtg cccgcgggga gcgatgcgcg ccagcaggcg gttggccgga ttctccaggt
     8941 cactcatcag ctcgcaatgg aacctcgccg gctgatcgga gaagtaatcc ggcaggtagc
     9001 gcagcgccca ggccagggac tggggcatca gcaactctac ggacgattca ctcggcatgc
     9061 actggcaatc ctgcttttgg gccataattc accagtgaac cgtaggagca cggtcggtag
     9121 aaaagctaga aagggggtcc acattgtggg cgggtgtgcg gctgtgcgga ctagtggcgg
     9181 aaaaaggcga ctgccgggga aaaaccgacc cagactgaac cagcctgtcg ttcgcgggcg
     9241 cgctccctag aatcgagcgc gagatgttgg cagaaagcgc gagtgtgctt cgcgcacggg
     9301 aagattatgg atggctttgc tcgtgaccac gcgcgtggcg ctgtgcggag cctgccgcgg
     9361 gcggcacgag tcgcggcggg cgcgctcgcg ttgttgacgg cgctgttccc cgcctgccgc
     9421 cgccaaggcg catcgcaaaa cgccgaagcg gcccgcgcct tgccggcccc gattacggtc
     9481 gccaccgcgg aaattgccga cgtacctatc gcgattggag tcgtggggcg agcggaatct
     9541 gtcaacaccg tcacgatcac gccgcgggtc agcgggcaag tcgacgacgt gcacttcatg
     9601 gacggtcagt acgtggaagc aggaacgttg ctgtacacca tcgatcaacg cccctttcag
     9661 gcagccgtgg accaggccaa agccaccctg gcgcgtgata aggccttggc ggcggaggcg
     9721 gcgaagaagg cacagcagta tgagcaggcc taccgaacca aggcggccgc cgaggtggat
     9781 cgcgataccg cggtcgccaa tgctgcggca acggcggcga cggtcgaggg cgacgaggcc
     9841 tcactggaaa acgcactgct gcaactcgaa tggtgcacga tccggtctcc gattgcaggg
     9901 aaggtgggct atcggctggt agatgtcgga aacatagtga cggctaatca gacggcactg
     9961 gtggttatca atcaaatcga gccgatctac gtcactttcg ctatcccgga acgatacctg
    10021 gctgaggtgc acgcgcgcct gaattccgga ccgcctgagg ttgaggcgac aattccgggc
    10081 gacagcggcg tcgtcaggcg cggcgagctg acgttcgtag acaaccaggt caatccagcg
    10141 accggcgcca taacgctgaa aggcactttt gccaacactg atcgcgcgct gtggccgggt
    10201 caatttgtaa acgtgaacct gattctgggg gtccaggcgc gcgtcgtaat tccgagtgaa
    10261 gcaatacagt cggcgcagca gggcgagttc gtctatgtat tgacgccgga cgacaccgtc
    10321 gagttacgga ccgtggtcac cggacgcacg ctggaagggc gcaccgtaat aatggaaggg
    10381 ctcaaagccg gcgaggtcgt ggttaaagag ggtcagacac gcctgcaacc gggggccaag
    10441 gtttcggtgc aacccgcggc cagccggccc acgaacgcaa actcgagtgc tcctaagaaa
    10501 acgggcgtaa cgcgtacccg agataacggt cttcagccct agggaatcag cagcgctgag
    10561 cgacagaggc aggctgggat gttttcgcgc ttctttatcg agcgaccgat cctggccaat
    10621 gtgatcgccg tgataacgat gttgctgggg ggcgtggcct tactggtatt gccggccgag
    10681 ctatatccgc aaatcacgcc gccgacggtc agggtgtccg cgaattatcc gggcggcagt
    10741 gcaaaagtgg tggcggatac ggtgggagcg ccaatcgagc agcaagtcaa cggcgtggag
    10801 aacatgctct acatgtcctc gacttcatcg aacgatgggt cgtacgcact gaccatcacg
    10861 ttcgaggtgg gaacgaacat cgacatggcc caggtgctgg ttcagaaccg ggtgcagatt
    10921 gcgctggcgc agcttccgcc ggaagtgcag cggcaaggga taaccacgct caagcagtcc
    10981 accgacatcg tcatgttcgt gacgctcacg tcgccggacg agcgctacga cagcctgttc
    11041 ctcagcaatt acgcgacgat acacattcgc gacgcgcttt cccggattcc gggcgtgggc
    11101 caagtgacgg tgataggcgc gggcagctac agcatgcggc tgtggttgga cgcggacgaa
    11161 ttggcagccc gcggtctgac gaccctggac gtggtaaacg cggtcgagga gcagaacgtg
    11221 caggtagcgg ccggacagat tggccagccg ccggccccac ccgaccaggc gactcagctc
    11281 accgtaacca cgctgggtcg tctgcgcacg ccggaacaat ttggcgacat tattgtcaag
    11341 acaataactg ctccgacgcc gcagatagtg cgtgtgcggg acgtggcgcg cgtggagctt
    11401 ggggcacaga cgtacgacca gtttttccag gtgaacggcg taccggcggc cggcatggcg
    11461 gtgtttcagc ttcccggggc caacgcactg gacgtggcgg acagaatccg tgccgagatg
    11521 cagcagttgc gcgagcagtt tccgccgggc ttgcgctatg acatcccttt cgacacgacc
    11581 ctgttcgtgc gggccgccat tcgtgaacta tactttacgt tgcttgaagc cggcgtcctc
    11641 gtcttgctgg taatcatggt tttcttgcag gactggcgtg ccgtactggt gccggcgacc
    11701 accgttccgg tcactctcgt gggcgcattc atcgcgctgt tcgccttcgg attcagcgtc
    11761 aacctgctga cgctattcgg gctggtgctg gcggtgggca ttgtcgtgga cgacgcgatt
    11821 gtggtgactg agaatgccgc gcaccacatt gaaagcgggc agcagcctaa agaagcggcc
    11881 attgcggcca tggttgagct gtttggtccc attatcggca tcaccctggt attgacttcc
    11941 gtcttcctgc cggccgcgtt cctgcggggc atcaccggcc agctctatcg ccagttcgcg
    12001 ttgaccatcg ccgccacggc cgtgatcagc gctataaacg cgctgacgct caagccggct
    12061 caatgcgcgt tgtggctgcg tccggcggtc cagcgtccgc gattcatctt ctttcgcgcg
    12121 ttcaacagtt tttacggtcg ggttgagcat gcgtacgcgc gcagcgtaca ctggatgacg
    12181 cgccgctcgg cgctgttcat gattccgtac gccctgctgg tagccgtaac gttgtggggc
    12241 ttcctggcgc tgccaaccgg ttttctgccg atcgaagacc agggctattt catgaccagc
    12301 gtgcaactgc ccgacgcggc ctcacaggac cgcaccaagg ccgttgtgga cagattgacc
    12361 gacattttgc attcgacgcc gggaaccaag aatgtgaacg cgctgatcgg gcaaaacatt
    12421 tttgagcaga cggtggcctc caatgcggcc gcgatttaca cgacgttgca gccgtgggat
    12481 gagcgcggcc cggctgaaag ccaggaggca atactcgaac acgtgagcaa gatgtacagg
    12541 gcgctgccgc aggcgacagt catctcgttt ccgccgccag ccattcgggg actgggggta
    12601 acgggcgggt tccagatggc ggtgcaagac cgcggggacg tagggctggc ggaattgggg
    12661 caagcggcgc gcgcgctgat catggccgcc aacgcgaccc cggagctgcg cggcgtgaac
    12721 accaccttca gcgaccgagt accgcaaatc tacctcaaca ttgaccgcag caaggtaaaa
    12781 tcgctgggcg ttccgctcag cagcgtattc aatacgcttc agacttacct gggctcgaat
    12841 tacgtcaacg atttcaacct gttcgggcgc acttaccagg tgcgcttgca ggccctgccg
    12901 cactttcgcg acaagatcgc ggacgtgctg gggctgtatg tccgcaacga gagcggcgaa
    12961 gtcgtgcagc tcggcacgct gctgaacgtg gagcaccgcg cggggccgca gacccttacg
    13021 cgatacaacc tctatcccgc cgcttcgatc accgggcagg ccgcaccgca cgtcagctcg
    13081 ggccaggcgc tggcgataat ggagcgcctg gcggacgaac atctgccgcg cggcgtcggc
    13141 tttgaatgga cggccacgtc gtaccaggaa aagcgcgtcg gacgccaggc gttgattgtc
    13201 ttcgcgctgg cggtcagcct ggtttacctg gtgctcgctg gtcaatacga aagctggagc
    13261 gacccggccg ccgtcattct tgtggttccg ctggcgctgc tgggggccgt ggtcgcggtc
    13321 gcgttacgcg acatgccgaa cagtatttac acacaggtag gcattgtgct ggtgatcgcg
    13381 ctggccagca aaaatgcgat tctgatcgtg cagttcgcgc gcgagcagcg ccgaaggggt
    13441 atgagcattg ccgatgcagc ggcggaagcg gcgcgtctgc ggttgcggcc gattctgatg
    13501 acctcgtttg cgtttatttt tggcgtggtg ccgctggtga tcgcgaccgg ggccggcgcg
    13561 gccagccggc gtgcgttggg aacggcggtc tttggcggca tgattgcatc cacgctactg
    13621 gcggtgtttc tggtgccggt attcttcgtg gtatgcgaac ggctgagtga gcggctgagg
    13681 cggaaagttt gagcatcagc caccgaaggg ggccggcgct gtagattagt actgtgagcg
    13741 aagactctgg ccacgaatgc ggcgttgaac gagagtatga caagctggcc gacgaggcct
    13801 tgatggcgct ggtcgcacag ggccggcgtg aggccttcac tgtgcttgtc gggcgacatc
    13861 aggcgggggt gatgggcgtc gcctatcgat tcatggggca gcgggacgta gctgaggatg
    13921 tcgcgcagga tgtgttcctg aaggtgtggc gctccgcatc tgccttccgc cccgaggcgc
    13981 aattcacgac ctggttgtac cggctgacgg caaacctctg ctgggatagg cgacggcgtg
    14041 ccgctcgcga agcgcgcttt cggatcgcct ttttgcagac gcggaccgct agcgagccgc
    14101 cttcaaacct cgacagtcat gaacgggtcg agctcgtccg gcgggccgtg gccagattgc
    14161 cggaccgaca acggctggca gtgattctgc atcgctatgc cgggctttcg catcgcgaaa
    14221 tcgcggaagt gacggggtgg agcgtgaagg cggtggaatc gtgcctcgtg cgcggttacg
    14281 gggcgctacg caagtcgctt gctcaactgg attcgcgccg cgcgaaccag gcgcgataaa
    14341 tgttttttcg gactcttttt ctgccccagc aaaaatttac cgcaggttga gccgaggttg
    14401 agcgtttaag ggatggagat gcactcatga aacacgcgcg gcgactagac ctgatccggt
    14461 tggcggccga cgagctgccc gagcaggacc gcgccgaggt cgaacgtcac ctagcgacgt
    14521 gcccggcctg ccgcagtttg tacgagcagc aggccgcgac gtggcgagcg cttggcgagt
    14581 gggcgcccga gaccgcgcac gtcgatttgt tggcgcagat tgaccgcaag ctgtctgaaa
    14641 ggccggccac gagacccccg ttctg
//
LOCUS       DAOSYS010000066        14660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3458_length_14660_cov_5.508661, whole genome shotgun sequence.
ACCESSION   DAOSYS010000066 DAOSYS010000000
VERSION     DAOSYS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_14660_cov_5.508661"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1469
                     /gene="recA"
                     /locus_tag="PLU99_05770"
     CDS             366..1469
                     /gene="recA"
                     /locus_tag="PLU99_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOO16601.1"
                     /translation="MVDAREDNRKEALGRALQQIEKAFGKGSIMFLKGEALRVEDGIS
                     TGSISLDLALGGAGIPRGRVIEIFGPESSGKTTLTLHVIAEAQRTGGAAAFIDVEHAF
                     DPSWAKRIGINLEELLINQPSCGEEALEICELLVRSNALDVIAVDSVAALVPRAELEG
                     QMGEHQVGAQARLMSQAMRKLTGISSKSRTAVIFINQIREKIGVMYGSPETTPGGRAL
                     KFYSSVRIDTRRTGMLKEGEQVIGTRVRAKVVKNKVAPPFREAEFDIYFDGGISRESD
                     LLDVAAECGVLARSGTWFSYGELRLGQGRENARQFLKDNPDLVRDIRKKVLDIKLPKP
                     EPQSGKSESDDGQPQKPEAARATASAGKQPRKR"
     gene            1651..2907
                     /locus_tag="PLU99_05775"
     CDS             1651..2907
                     /locus_tag="PLU99_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO16602.1"
                     /translation="MTRSCQEIHCSRLRGLGLWFALVGLLAWPYYFGVPALAEEAESA
                     QLVGAADLMANLEAVEQAFSRIVEQVSPCVVGIRARRYESSSPYERGGYATQNGFEQT
                     VTINGSGVVLRPDGSILTNEHVVRGADEVEIILRDGSRRVGQVVAADPRADLAVVRTE
                     PDGLVPARIAEWDNVRRGQWSIAVGNPFGLGYDGNLSVSVGVISNLNRRLPGLGEVED
                     RLYADMIQTTASIHPGNSGGPLFNMRGEVVGIVTAVHSRGIDDEGIGFAIPMSPARRQ
                     LIDMLAAGKPVEHGFLGLIVRELNPNERMAAGLSPDVGVWVDRVEPNGPADAAGIRPH
                     DIIVRYDGRLVEAPMQFADCVGLTPIGQQVTLEIIRRNQSLTLQATLDRRQSSRVQSI
                     RGASALWRGLHHGSDLASDAEAVQDL"
     gene            complement(3025..3930)
                     /gene="lipA"
                     /locus_tag="PLU99_05780"
     CDS             complement(3025..3930)
                     /gene="lipA"
                     /locus_tag="PLU99_05780"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOO16603.1"
                     /translation="MHEQQSPAEDSGPAVHQRRLPPWLKRPVPVASALLHTRKLINGL
                     RLNTVCVEARCPNLTECWSRHTATFMILGDTCTRHCGFCAVNTGRPEPPDDGEPQRLA
                     EAAAHLNLKHVVITSVTRDDLPDEGAGHFARCIRAVQQQLPQSTIEVLVPDFHARPEC
                     VQAVAEAGPEVFNHNLEVVEAQQKRIRPQADYRRSLRTLELAKEHRPSLRTKSGLMVG
                     VGETRAELLQAFKDLRAVGCDFLTIGQYLKPKAGAAHVPVERYYPPDEFRELGDLAKE
                     LGFLAVASGPFVRSSYLAETLYHQP"
     gene            4055..4822
                     /locus_tag="PLU99_05785"
     CDS             4055..4822
                     /locus_tag="PLU99_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOO16604.1"
                     /translation="MPHEPLDIIFAMPHPDDVEITCGGTIARLVDLGYRVGVLHMTNG
                     EPTPRGTPQVRAAELAEASRILGVTRVDTLALPNRELMDCPAARYAVATVFRRHRPRI
                     VVGMAGRTPGASPDHYQAQLILEAARFYAQLTKWDERFDNTPPHRVDWLWYRPVHIAA
                     DIPHWHATFVVDVSAVYDKKLAAIAAYKSQFDAERLARLLHRIRAFDAADGARAGFQY
                     GELFALPHPLPMPDPLAYFAPLVPPQGPPPSVKPNNA"
     gene            5112..6818
                     /locus_tag="PLU99_05790"
     CDS             5112..6818
                     /locus_tag="PLU99_05790"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO16605.1"
                     /translation="MLRELIDISQQEDFALGLSRISGLRVCTFDTDGRLIVAGGPISG
                     FARLTGHTTPLIPAGVELVPVPAHDPPAAVAFIPNGGSWYIAAPVYCDDQKAGWVAVG
                     EFREDGQAPSQLPDVLGLWNMLPLLDRRGTSHSVITARWGARLLAEWCRREARVSAAT
                     QEAALIGDVAELLTGRLELDAILDRIVADTARVMRCEFCSLRLYDPKTNELRIKAVYG
                     LSKRYIGKGAIVRYESSIDDEALSGRMVYVEDAAQDPRIQYPEEMRREGIKSFLTAGL
                     IYRGEPIGVLRVYTNRRQRFRRTQRHLLRAVAHQAAMAIVHARMLQERLRSAETERQL
                     ALASDLQARMMCVPAPERSGIVTARVYQPSFGLGGDFCDIFTLCDGRLTAVVADVVGK
                     GIPASLLSSMVRGALYATAQGCDDLGELLNRLNRQICGETLPSEFVTLLVIAVDVERR
                     LLGYASAGHEPLLLMRQGVVREYGNGSMVLGIDATETYREHRVELQTGDMILLYTDGL
                     VEAMNFSGELFGRERLFAALREYGGLALDQVLQSILWDLRRFVGLAEQSDDVTMIGLR
                     LV"
     gene            complement(6893..7651)
                     /locus_tag="PLU99_05795"
     CDS             complement(6893..7651)
                     /locus_tag="PLU99_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOO16606.1"
                     /translation="MLDFDRYSRQIPFPGLGEAGQRRLAEARVLLVGCGGLGSVQATL
                     LVRAGLGWLRIVDCDAVEVQNLHRQILYDEPETGRPKAEVAAAKLRQANSAVQVESVV
                     ARFAAANAEELARGADLILDGTDNAATRFVINDVAVKHGLPWIYGACVGAEGRMLPII
                     PHRTACLRCIWDEPPAHPTCATVGVLGPMVAMVASFQALAAMQVLIGRVDDLDTRMLV
                     IDGWSWRMRRVDVKAALERGDCRCCKQGAYEYLR"
     gene            complement(7657..8139)
                     /locus_tag="PLU99_05800"
     CDS             complement(7657..8139)
                     /locus_tag="PLU99_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16607.1"
                     /translation="MSSRPDLESPERGEQRDGEGAIRSTAFGTLLAGAVCLYFGFVWA
                     ADAPVSASAEAEQMWYLVDRVFRWWFGVVGVGFLAAAGLSALGKRTGALLSTAAEAGF
                     SLLMFALVVNGVAESVAMGGFDAGVILFVILLLIGLSATRHSWRVFKATGAGGDVRAG
                     "
     gene            8302..9681
                     /locus_tag="PLU99_05805"
     CDS             8302..9681
                     /locus_tag="PLU99_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOO16608.1"
                     /translation="MRQRKASKTPRSRTKRFYLAGGLVLVLALAAALTAALRARPAKT
                     PPNVVLIVLDTVRDDCVGAHWQGVPLTPSVDAIAAEGTRFTNAFSAAPWTVPSHATLF
                     TGLHPHRHYADHEHFKLADSFTTLAERLQAHGYATAGVTCNPWLTHTLGFKQGFEQYF
                     EVYADSQPNENDSPKASTQAIEWLRQRAADQRPFFLFINYLDAHLPYTPAPEALNRLP
                     PVPDAKPRTAFSIAEAERFLGRLDQLSPADLQEVRRVYAAEILAQDAQVARVLDYLRG
                     TGQLENTVLIITADHGEQLGEHGFTGHEFSLYEPVLDVPLIVRFPPVFPAGLQTPAPA
                     GLIDILPTILDVAGLPPPSERLPGCSLRQLLVAPAAYRPLLAEYARPVTLITKYWRAN
                     QPRVDMSAYDTSLKSLRLDSFKYIAGSGWERLYDLSTDPGEEHDLSAEMPEQLAEMRA
                     QLAQLELGP"
     gene            9746..10702
                     /locus_tag="PLU99_05810"
     CDS             9746..10702
                     /locus_tag="PLU99_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO16609.1"
                     /translation="MVAAAQPAYLAPQVRDGLDRRVAATLEQLRCCRTCPRDCQVDRL
                     TGKTGFCRTGRHAIVSSAFAHHGEEDCLRGWRGSGTIFFSMCNLGCIFCQNWDISHRP
                     SGQEVDAAKLADLMLALQSEGCHNINLVTPSHVAPQLVEALALAIERGLRLPVVYNTS
                     AYDSVNTLRLMDGLIDIYMPDFKFWRAETANQLTAAGDYPERARDAIREMHRQVGVLK
                     LSADGLACRGVLVRHLVMPGQVAESRAIFRWLATEISPDTYVNIMAQYTPAYAAVRAG
                     GATRQSPADIDRRPTRQEIEEAFAAAREAGLWRFDAPNRQRV"
     gene            complement(10718..13060)
                     /locus_tag="PLU99_05815"
     CDS             complement(10718..13060)
                     /locus_tag="PLU99_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO16610.1"
                     /translation="MLISAVTIAFLFLAAPGNQQELTKEQLEELPSYFGFGPMQIYKL
                     DPEIALLELADLNQDGRTDVALWNPHKSRFELFYQPDPDKPPAADVEHLERNEIASRG
                     DLQREHVAVPYRVASMKIADVTGDGRPDLIFFGEPRELVILPGKAGGGFEAPDAVRAP
                     EGEPRNSFVQIGDFNHDGRTDVALLGGEVLHIFYQKAGGGLDKPVRLVHGLRQPLLML
                     TADLNGDGRDDLAIGLDDDQYGLCVYLQDEGGGFSALRRIKVPRLRSMTFADGSQGSD
                     LFSVEAVTGHLKEYRWQVQEDSAGTPDWPQRLYSYPIKMEGKRVPLAIGDLTGDGLPD
                     CVAADSAGSQLVFLPGTQEGFGAGKAFPGLVKTVDLQIADIDNDGRPELLSVSRAERT
                     LGVSHFADGRLTFPKPLPIKGEPLVVAVGTLKAGSGEPVLAYVARERPGEQQAAEDEE
                     AGSEEEQESAPVAIRLTKPGTGEELQRIACELEDDPNGLRFVDVNHDGLNDLLLFTRF
                     AMLQTFLQDSRGRFAPLVGREARSGLVRDARPEAFCAVDVNADNQPEVILSQKNLARA
                     LVVEEGRWNVVDQYNADSSGAEITGLTALPGEAGSPTLVLYDRKGRNLLVLKRGSDKT
                     YSVVQTMPVGDYDVSAMTTLAIGQQGRPGVVLADSEKLALFRPDEWAPTLVAQHSYET
                     DVKDGWLGDAAIGDLNHDGVRDVLAIDIRKASLDILTEAPDGKLVRALRFQVFQGKRF
                     SSDPNQMGEPREVRIGDVTGDGIADVVLLVHDRLIVYPGQ"
     gene            complement(13174..14070)
                     /locus_tag="PLU99_05820"
     CDS             complement(13174..14070)
                     /locus_tag="PLU99_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16611.1"
                     /translation="MSRTFFVLFSGMAAFFASSAQASDLEAVQKAIQEAWGRHSSMTA
                     RMRMESHRAQGDAVHEGRGEGIVEVLRKGSLYVQRKEVHTEMTRTAADPQESAFMPYD
                     ATIILDGESEYLIRGSGDQKFYTKVPVNPGMSVVPQTVIEFLGQSAELKALPEQTIDD
                     QKVWVIEGVRSEPNPKTKTLRTVLYFLQDSGALRRQEEFDAAGVKLETIDYVDFQFDV
                     ELDPQRFVLELPPGARLIDKTQKRTPTTGPATQPGEAAGQPIWSSPIRGYLGAPASQP
                     STPAPSTAPSSAPAAPVRPDRT"
     gene            14385..>14660
                     /locus_tag="PLU99_05825"
     CDS             14385..>14660
                     /locus_tag="PLU99_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOO16612.1"
                     /translation="MGKIRVAIAGVGNCASSLLQGIEYYKHSRSNETAGLVHPKIGGY
                     EIGDLQPVAAFDIDRRKVGQPLEKAVFAPPNCTTVFQPELPAYGVTVQ"
BASE COUNT         2682 a         5019 c         4435 g         2524 t
ORIGIN      
        1 tgacatcgac cgcttcatag atgccatgcc caagctgtaa gcgcgggcat gccctcgtca
       61 acggcgggat cgctggtgat ggcatgcccg cacccgcggg cgcggcagtc aaccctcctt
      121 cccagcccgt tcggccagtc ctgcggtgtg actctcctgg gtgtctcatg tcccaccttg
      181 ccacgatccc gaacaagtaa gggtcacaca cgccagatta cagcccacaa agattacagc
      241 cggccgcgcg aaaaaagcgt tgactgttag gccgctgttt atagaatgac aacgccgtaa
      301 actatgaaca ggggtttatg ccggcggacc gaccggtttt ttccgccgaa ggagcagtaa
      361 ccaggatggt cgacgcgcgc gaggacaacc gcaaggaagc gttggggcga gcgcttcagc
      421 agatcgaaaa ggccttcggc aagggctcga tcatgttcct caagggcgag gccctgcgag
      481 ttgaggacgg catttcgacc ggctcaattt cgctcgacct cgcgctgggc ggagcgggga
      541 ttccgcgcgg gcgcgttatc gagattttcg gccccgaatc ctcgggcaag accacgctga
      601 cgttgcacgt gatcgccgaa gcgcagcgca ccggcggcgc ggcggcgttc atcgacgtcg
      661 aacatgcttt cgacccgagc tgggccaagc gcatcggcat caacctcgaa gagctgctga
      721 tcaaccagcc ctcctgcggt gaagaagccc tggaaatttg cgaactgctg gtacgctcaa
      781 acgcactcga cgtgatcgcg gtcgactccg tcgccgcgct ggtcccgcgg gccgagctcg
      841 aaggacagat gggtgagcac caggtcggcg cccaggcccg gctgatgagc caggccatgc
      901 gcaagctgac cggtatctcc agcaagagcc gcacagcggt catctttatt aaccagattc
      961 gcgagaagat cggcgtcatg tacggctcgc cggagaccac tcccggcggg cgggcgctga
     1021 agttctattc ctcggtccgc atcgacacgc gccgcacagg catgctcaag gaaggcgaac
     1081 aggtgatcgg tacgcgtgtg cgggcaaagg tggtgaagaa caaggtggcc ccgccgttcc
     1141 gcgaggccga gttcgacatt tattttgatg gtggtatcag ccgcgagtcc gacctgctag
     1201 atgtcgccgc cgaatgcggc gtccttgcac gcagtggtac ctggtttagt tacggggaac
     1261 tgcgactggg gcagggacgc gaaaatgcgc gacaatttct caaggacaac cccgatctgg
     1321 tgcgggacat ccgcaagaag gtcctggata tcaagctccc caagccggaa ccacagtccg
     1381 gtaagagtga atcagacgac gggcagcctc aaaaacctga ggctgcgcga gctacggcaa
     1441 gcgccggcaa gcagcctcgc aagcgataga gcgtccgtca cgccattagt cccccgccgc
     1501 aaaaccgagc gcgccgccgg tgcgcgccgg ggcccggggg cgctgatcgg cctgcttgaa
     1561 tcggtcgaaa ttaaacaggg aaaggaagtc gtaggcgccg cgatcgcgag ccggcgaatg
     1621 cgcctgaacg aactgggagg aatttcatta atgaccaggt cgtgccagga aatccattgt
     1681 agcaggttac gggggctggg cctgtggttt gctcttgtcg gcttgctggc gtggccttat
     1741 tacttcggtg tgcccgcact ggcagaggaa gccgaaagcg cccagcttgt cggggcggcg
     1801 gacctcatgg ccaacctcga agccgtcgag caggcattct cgcgcatcgt agagcaagtg
     1861 tcgccctgcg tggtcggcat tcgggcgcgg cgctatgaaa gcagctcccc ctacgagcgc
     1921 ggcgggtacg caactcagaa cggcttcgag cagactgtga cgatcaacgg cagcggcgtc
     1981 gtgcttcgcc cggacggctc gattctgaca aacgagcacg tggtgcgcgg cgccgacgag
     2041 gtcgagatca tcctgcgcga cggctcgcgc cgcgtgggac aggtggtggc cgcagatccg
     2101 cgcgccgatc tggccgtcgt gcgaacggag ccggatggtt tggtgccggc caggattgcg
     2161 gaatgggaca atgtgcggcg cggtcagtgg agcatcgcgg tcggcaaccc cttcggcctg
     2221 ggctatgacg gcaatctcag cgtttccgtg ggcgtgattt ccaacctcaa ccgccggctg
     2281 cccggcttgg gcgaggtcga ggaccgcctg tacgcggaca tgattcaaac gacggcttcc
     2341 attcaccccg gcaactcggg cggcccgctc tttaacatgc gcggcgaagt cgtcggcatc
     2401 gtcacggccg tacattcgcg cggcatcgac gatgagggca tcggtttcgc gatacccatg
     2461 agcccggcgc gccggcaact catcgatatg ctggcagccg gtaaaccggt cgagcacgga
     2521 ttcctgggcc tgatcgtgcg tgaactgaac cccaatgagc gcatggcagc cggactcagc
     2581 ccggatgttg gcgtgtgggt ggatcgtgtc gagcccaacg gcccggccga cgcagccggc
     2641 attcgcccgc atgacatcat cgtgcgctac gatggtcgcc tggtcgaagc cccgatgcag
     2701 tttgccgact gcgtcggcct gacgccgatc gggcagcaag tgacgctcga aattattcgc
     2761 cggaaccagt cgctgacgct gcaagccacg ctcgatcgcc gccaaagcag ccgtgtgcag
     2821 tcgattcgcg gcgcttcggc cttgtggcgc ggcctgcacc acggatccga cctggcgagc
     2881 gacgccgagg ctgtccagga cctctaggca gagacgagcc gggcgtatcg cgccccgcag
     2941 tctagggaac agaagtaatc gggggcatac tccggaggca tgcccgcgcc cgctgcgcgg
     3001 gcttgggcat ggcacccggg acgatcacgg ctggtgatag agcgtctcgg ccaagtagct
     3061 gctgcgcacg aacgggccgc tggctaccgc cagaaagccc agctctttcg ccaaatcccc
     3121 taactcgcgg aattcgtccg gcgggtaata acgctccacc ggcacgtgag cggcgccggc
     3181 tttcggcttc aggtattgac caatcgtcag gaaatcgcag ccaacggcgc gcaggtcctt
     3241 gaacgcctgc agtaattccg cccgcgtctc acccacgcca accatcaggc cgctcttggt
     3301 gcgcaggctg ggacgatgct ctttggcgag ttccagcgtc cggaggctcc gccggtaatc
     3361 agcctgcggg cggatgcgct tctgctgggc ttccacgact tcgaggttat ggttgaagac
     3421 ctccgggccg gcttcggcca ccgcctgcac acattccggg cgcgcgtgaa aatcgggcac
     3481 caacacttcg atcgtgcttt gcggaagctg ctgctgaacc gcgcgaatgc agcgggcgaa
     3541 gtggccagcc ccctcgtccg gcaggtcgtc gcgcgtgacg gaggtaatca ccacatgctt
     3601 caaattcagg tgcgcggcgg cttccgccag acgctggggt tcaccatcgt cgggcggttc
     3661 ggggcggccc gtgttgacgg cacaaaagcc gcaatgccgc gtgcacgtat cccctaagat
     3721 catgaacgtc gcggtatgcc gggaccagca ctcggtcagg ttcggacagc gtgcttcgac
     3781 gcataccgtg ttcagccgca gtccgttgat gagcttgcgc gtgtgaagca gcgcgctggc
     3841 gaccgggacc ggacgcttca gccaaggcgg caggcgccgc tgatggacag cggggccgga
     3901 gtcctcggct ggagattgct gctcgtgcat cctgcaaatt atacgtggcc cggcccgggg
     3961 tgcgactgct cgtcgccggc aagccgctca ggcgatatgg taacaggtga cccgcgccgc
     4021 gtgccggatc gaaacaacgg cgaaaggagc aagcatgccg cacgagcctt tggacataat
     4081 cttcgccatg ccgcaccccg acgacgtcga gatcacctgc ggcggaacta tcgccaggct
     4141 ggtggacttg ggatatcgcg taggcgtgct gcacatgacc aacggcgagc cgacaccgcg
     4201 cggaacgccg caggtgcgtg cggccgaatt ggcggaagcc tcgcggatac tgggcgtgac
     4261 ccgcgtagac acgctcgcgc tgcccaaccg cgagttgatg gactgccccg cggcgcgcta
     4321 cgcggtcgcg accgtctttc gccggcaccg gccgcgaata gtggtcggaa tggcaggccg
     4381 caccccgggc gcctcgccgg atcattatca ggcccagctc atactcgaag cggcccgctt
     4441 ttacgcccaa ctgacgaaat gggacgagcg cttcgacaat acgcccccgc accgtgtgga
     4501 ctggctctgg taccgcccgg tgcacatcgc cgccgacatc ccgcattggc acgcgacgtt
     4561 cgtcgtggac gtgagcgccg tgtacgataa gaagctcgcc gcaatcgcgg cgtacaagtc
     4621 gcaattcgac gccgaacgac tggcgcggct gctgcaccgc atccgcgcct tcgatgcggc
     4681 cgacggggcg cgggccggat tccagtacgg cgaacttttc gccctgccgc atccgctacc
     4741 gatgccggac ccgctggcgt acttcgcccc gctggttccg ccacagggcc cgccgccgtc
     4801 ggtcaagccg aataacgctt gaagcgcagg gttggttccc gccggagcgc actttcccac
     4861 attcctcacg ttcccacttc cccgccggag cacactttcc caccttccca ccttcccact
     4921 ttcccacact cacacgtggg aacgtgggaa agtgggaacg tgggaaagtg ggaaggtggg
     4981 tgtgaacgtg cggggacgtg tgcaggcgcg aacgtatgaa tgtgtggggc gcttgatccg
     5041 tctgcctgct aagcgatacc ctgttttcgg tccggcttgg taggctaagg ccgcggaggc
     5101 aacgctcgcc catgcttcgc gagctcatcg atatctctca gcaggaggat ttcgctcttg
     5161 gcctcagccg tatcagcggc ctgcgcgtct gcaccttcga caccgacggc cgcctgatcg
     5221 tcgccggtgg acctatcagc ggctttgcac gcctgaccgg gcacaccacc ccgctcattc
     5281 cggccggcgt ggaactcgtt cccgttccgg cccatgatcc gcccgccgcg gtcgccttta
     5341 tccctaacgg cggaagctgg tacatcgccg ctccggtcta ttgcgatgat cagaaggccg
     5401 gctgggtcgc ggtcggcgag ttccgcgagg atggccaggc cccctcccag cttccggacg
     5461 ttcttgggct ctggaatatg ctgccgctgc tcgaccggcg cggcacctcg cattccgtca
     5521 taaccgcccg ctggggcgcc cgcctgttgg ccgaatggtg ccgccgcgag gcgcgcgtca
     5581 gcgccgccac gcaggaagct gcgctcatcg gcgacgtggc cgaattgctc accggacggc
     5641 tcgagctgga cgccatactg gaccgcatcg tggccgacac cgcacgcgtc atgcgctgcg
     5701 agttctgcag cctgcggctg tatgacccca agaccaacga gctgcgcatc aaggcggtct
     5761 acggcctgtc caagcgctac atcggcaagg gggcgatcgt acgctacgaa agctcaatcg
     5821 acgacgaagc gctctcgggg cgcatggtat acgtcgagga cgccgcgcaa gacccgcgca
     5881 ttcaatatcc cgaagagatg cgccgcgagg gcatcaaaag ctttctcacc gcgggtctga
     5941 tttaccgcgg tgagcccatc ggcgtcctgc gcgtctacac caatcgccgg cagcgctttc
     6001 gccgcacgca gcggcacctg ctgcgcgccg tggcgcacca ggcggccatg gccatcgtac
     6061 acgcgcgaat gctccaggag cgtctgcgca gcgccgaaac ggagcgccaa ctcgccctgg
     6121 ccagcgacct gcaggcccgc atgatgtgcg ttccggcccc ggagcgcagc ggcatcgtca
     6181 cggcccgcgt gtaccagccc agttttggct tgggcggcga tttctgcgac atcttcacgc
     6241 tgtgcgacgg ccgcctcacc gcggtcgtgg cggacgtggt cggcaagggc attcccgctt
     6301 cgctgctcag ctccatggta cgcggagcac tctacgcgac cgcccaaggc tgcgatgatc
     6361 tgggcgaact gttgaaccgg ctgaatcgcc agatctgcgg cgagaccctg ccgtcggaat
     6421 tcgtaacgct gctcgtgatc gcggtcgacg tcgaacggcg gctgctcggc tatgccagcg
     6481 ccggccacga gcctttgttg ctaatgcggc agggcgtagt ccgggagtat ggaaacggca
     6541 gcatggtcct gggcatcgat gccaccgaaa cctaccggga acacagagtg gaactccaga
     6601 ccggcgacat gattctgctg tacaccgacg gtctggtcga ggccatgaac ttctccggcg
     6661 agctattcgg gcgcgagcgg ctatttgcgg ccttgcgcga gtacgggggc ctggccctgg
     6721 accaggtgct ccaaagcatc ctctgggatt tgcggcgctt cgtcggcctg gccgagcagt
     6781 ccgacgacgt gaccatgata ggtctccggc tcgtgtagca gagccgcggc gtagcgcccc
     6841 ggaagtcgcc gcatcgcccc gaactgttgc cggtatcgcc cccgaaatgc ccctacctca
     6901 aatattcata cgcaccttgt ttgcaacacc ggcaatcacc ccgctccagc gccgctttaa
     6961 cgtcaactcg tcgcatgcgc catgaccagc cgtcaatgac cagcatccgc gtatccaaat
     7021 catcaacgcg accgatcaaa acctgcatcg ccgccagcgc ctggaagctg gcgaccatcg
     7081 caaccatcgg cccgagcact ccaacggtcg cgcacgtcgg atgcgccggc ggctcatccc
     7141 agatacaacg caagcacgcc gtccgatgcg ggataatcgg cagcatgcgc ccttccgcgc
     7201 ccacgcacgc cccgtatatc caaggcagac cgtgcttcac cgccacatcg ttaatcacaa
     7261 accgcgtcgc ggcgttatca gtcccgtcca gaatcaagtc cgccccacgc gccagctctt
     7321 ccgcattggc cgccgcaaaa cgcgccacaa cgctctcaac ctgcactgcg gaattggcct
     7381 gccgtaattt ggcggccgca acctccgcct tgggccggcc ggtctccggc tcgtcataaa
     7441 gtatctgccg gtgcaggttc tgcacttcga ccgcgtcgca atccacgatg cgcagccacc
     7501 cgaggcctgc ccgcaccagc agagtcgcct gcaccgatcc caacccgccg cagcctacga
     7561 gcagtacgcg ggcctcggcc aggcgccgct gaccggcttc tcccagaccc ggaaagggga
     7621 tctgccgact gtaacgatcg aaatccagca tctgtgtcaa cctgctctca cgtcgccgcc
     7681 ggcgccggtt gccttgaaga cgcgccacga gtgccgggta gcgcttagcc cgattaggag
     7741 cagtattacg aacaggatta cccccgcgtc gaaaccaccc atcgcaaccg attccgccac
     7801 gccattgaca accagtgcga acatgagcag cgagaagccg gcctcagccg ccgtactcaa
     7861 caacgcaccc gtgcgcttcc caagtgcgga caagccggcg gccgccagga accccacgcc
     7921 gactacccca aaccaccacc ggaagacgcg atctaccagg taccacatct gctcggcttc
     7981 ggcactggca gataccggcg cgtcggccgc ccagacaaac ccaaagtaca ggcagacggc
     8041 cccggccagc agtgttccaa aggcggtact gcgaatggcg ccttccccgt cccgctgctc
     8101 gccgcgctcc ggcgactcca ggtccggacg actgctcatg acctgcctcc aaaccgttaa
     8161 gccgccgatg ctatccggac gtacgggccc cgtcaccatg gacattcgcc gaatggctcg
     8221 cgtgtgcctg cctcccctct ggcccgcttg ccgcccgccc cctaacatgg gctctggtga
     8281 gcgaacttgt gggcgcgtag cgtgcgacaa cgaaaagcat ccaaaacgcc tcgaagcaga
     8341 acgaagcgct tctatctcgc gggcggcctg gtcctcgtac tggctctggc cgcagcgctg
     8401 accgcggcgc ttcgcgcgcg gcccgccaag accccgccga acgtggtctt aatcgtgctc
     8461 gacaccgttc gcgacgattg cgtcggcgcg cactggcagg gcgtgcccct gacgcccagc
     8521 gtcgatgcca tcgccgccga gggcacgcgc ttcaccaacg ccttctccgc cgcgccctgg
     8581 accgtcccgt cgcacgccac gctcttcacc ggcctccacc cgcaccggca ctacgccgac
     8641 catgagcact tcaaactggc tgactcgttc accacgttgg ccgaacgctt gcaagcccac
     8701 ggctacgcca ccgcgggcgt cacgtgcaac ccctggctga cgcacacctt gggcttcaaa
     8761 cagggattcg agcagtactt cgaagtctat gcggacagcc agccgaacga gaacgactcg
     8821 cccaaggcca gcacgcaagc catcgaatgg ctgcgccagc gcgccgcgga ccagcggccg
     8881 ttcttcctgt tcatcaacta cctggacgct cacctgccct acacgcccgc ccccgaagcg
     8941 ctgaaccgcc tgccgcccgt acctgacgcc aagccgcgca ccgccttctc aatcgccgaa
     9001 gccgagcgct ttctcggccg cctcgaccag ctctcgcccg cggacttgca ggaggtgcgg
     9061 cgcgtttacg cagccgagat tttggcgcag gacgcgcagg tcgcacgcgt gctcgactac
     9121 ttgcgtggca ccgggcagtt agagaacaca gtgctgatca tcaccgccga ccacggcgaa
     9181 cagctcggcg agcacggttt caccggacac gaattcagtc tttacgaacc agtgctggac
     9241 gtgccgctga ttgtccgctt cccgcccgtt tttcccgccg gcctgcaaac accggcgccg
     9301 gccggactga tcgacatcct gccgaccatc ttggacgtcg caggcctccc tccgccgtcc
     9361 gagaggctgc cgggctgcag cctgcgccaa ctgctcgtcg caccggccgc gtaccgccca
     9421 ctgctggccg aatacgcccg ccccgtcacg ctgatcacca aatactggcg cgccaaccag
     9481 ccgcgcgtcg acatgtccgc ttatgacacg tcgctgaagt ccctgcgcct ggacagtttc
     9541 aagtacatcg ccggcagcgg ctgggaacgg ctgtacgacc tttccaccga tcccggcgag
     9601 gagcacgacc tctccgcgga aatgccagag caactcgcgg agatgcgcgc tcaactggcc
     9661 caactcgaac tagggccgta agccgctcaa gctgccgccc ctgataaaat ctcgtcgcat
     9721 ggcgcctgca aaaccgagtc aattcgtggt tgccgctgcg cagcccgctt acctcgcacc
     9781 gcaggtccgc gacgggctcg accgacgcgt cgcggccacg ctcgaacaac tgcgctgctg
     9841 ccgaacctgt ccgcgtgatt gccaggtgga ccggctgacg ggaaagaccg gcttctgccg
     9901 aactggaagg catgcgattg tttcgtccgc cttcgcgcac cacggcgagg aagactgcct
     9961 ccgcggctgg cgcggcagtg ggacgatctt cttctcaatg tgcaacctcg gctgcatctt
    10021 ctgccagaac tgggatattt cgcaccggcc cagcgggcag gaagtggatg ccgcaaagct
    10081 ggccgatctg atgctcgcgc tgcaaagcga gggctgccac aatatcaacc tggttacacc
    10141 gtcgcacgtc gcaccgcagc tcgtcgaagc cctcgcactg gccatcgaac gcggcttgcg
    10201 cctgccggtc gtctacaaca ccagcgccta cgactcggtc aatactctgc ggctgatgga
    10261 cggcctgatc gacatctaca tgcccgactt caaattctgg agagcggaaa ccgcgaacca
    10321 gctcacggcc gctggcgact atcccgagcg ggcgcgcgac gccatccgcg aaatgcaccg
    10381 gcaggtgggc gtgctcaaat tgagtgccga cggcctggcc tgccgcggcg tgttggtgcg
    10441 gcatttggtg atgcccgggc aagtcgccga gagccgggcc atcttccgct ggctggccac
    10501 cgagatttca ccggacacat acgtaaacat tatggcgcag tacacccccg cctacgcagc
    10561 ggtccgtgcc ggcggcgcca cccggcagag cccggcggac attgaccgcc gtccaacacg
    10621 ccaggagata gaagaagctt ttgccgcagc gcgcgaggcc gggctgtggc gcttcgacgc
    10681 accaaaccgg caaagagttt gaaggccgtc gcccgcctta ctgccccggg tacacaatca
    10741 gccggtcgtg caccagcaaa accacgtccg cgatgccgtc acccgtgacg tcgccgatcc
    10801 gcacctcgcg cggttcgccc atctggttcg gatcactcga gaaacgcttc ccctggaaaa
    10861 cctgaaaacg caacgcccgc accagcttcc cgtccggcgc ctcggtcagg atgtccaacg
    10921 acgccttgcg tatgtcaatc gccaacacgt cgcggacgcc gtcgtggttt agatcgccga
    10981 tggccgcgtc gcccagccag ccgtccttga catcagtctc gtacgaatgc tgcgccacca
    11041 gtgtcggtgc ccactcgtcc gggcggaaca gcgccagctt ctccgaatcc gccagcacga
    11101 cccccggacg cccctgctgg ccgatggcga gcgtggtcat ggcgctcacg tcatagtcgc
    11161 cgaccggcat ggtctgcacg accgaatacg tcttgtccga gccccttttc agcaccagca
    11221 ggttccggcc cttgcggtca tacagcacca gcgtcgggct gcccgcttct cccggcagag
    11281 ccgtcaggcc cgtgatctcc gcgccgctgc tgtcggcgtt gtactggtca accacattcc
    11341 aacgcccctc ctcaacaacc agcgcccgcg ccaggttctt ctgagacagg atcacctccg
    11401 gctgattatc cgcgtttaca tcgacggcac agaacgcctc aggccgcgcg tcgcgcacca
    11461 ggcccgaacg cgcttcccgt cccaccagcg gcgcgaaacg cccgcgcgaa tcctgcagaa
    11521 acgtttgcag catggcaaaa cgcgtaaaca gcaagaggtc attcagtccg tcgtggttca
    11581 cgtcgacgaa acgcagcccg ttggggtcat cctccagctc gcacgcgatc cgctgcagct
    11641 cctcgcccgt cccgggcttg gttaaccgga tagcgaccgg cgcgctttcc tgctcctctt
    11701 cagatccggc ttcctcgtcc tccgcggcct gctgctcacc cggccgttca cgcgccacgt
    11761 atgccagcac gggctcgccg ctgccggcct tcagcgttcc caccgcaacg accagcggct
    11821 cccccttgat cggcagcggc ttggggaacg tcagacggcc gtccgcaaaa tgcgatacgc
    11881 ccaacgtgcg ctccgcccgg ctgacgctga gcaactccgg ccggccatcg ttgtcgatgt
    11941 cggcgatctg caagtccacc gttttcacca ggccgggaaa cgctttgccc gccccgaacc
    12001 cctcttgcgt accgggcaga aaaaccaatt gcgacccagc cgagtccgcg gccacgcagt
    12061 cgggcaggcc gtcgccggtc aggtccccaa tggccagcgg aacgcgtttg ccttccatct
    12121 tgatcgggta tgaatagagc cgctgcggcc agtcgggcgt gccggctgaa tcctcctgta
    12181 cctgccagcg gtactctttc aaatgccccg tgacggcctc gaccgagaac aaatccgagc
    12241 cctgcgagcc gtcggcgaaa gtcatacttc gcaaacgcgg cactttgatg cgccgcaatg
    12301 cgctgaagcc gccgccctcg tcctgaagat aaacgcacag cccatactgg tcgtcgtcca
    12361 gaccgatggc cagatcgtcg cgaccgtcgc cgttaaggtc ggcggtcagc atcagcaacg
    12421 gctgtcgcag cccgtgtacc agacgcaccg gcttatccaa accaccgccg gccttctggt
    12481 aaaaaatgtg cagcacctcg ccgccgagca gcgcgacgtc ggttcgccca tcatggttga
    12541 agtcgccgat ctgcacgaag ctgttgcgcg gctcgccctc cggagcccgt accgcgtcgg
    12601 gcgcctcgaa cccgccgccc gccttgcccg gcaggatcac cagttcgcgc ggctcgccga
    12661 agaaaatcag atcgggccgc ccgtcgccgg ttacgtcggc gatcttcata ctcgccacgc
    12721 ggtacggcac cgctacgtgc tcgcgctgca ggtcgccacg gctggcaatc tcgttgcgtt
    12781 caaggtgctc cacgtcagcg gccggcggct tgtccggatc gggctggtaa aacagttcaa
    12841 aacggctctt atgcgggttc cacagagcaa cgtccgtgcg gccgtcctga ttcagatcag
    12901 ctaactcgag cagcgcgatt tccggatcga gcttataaat ttgcatgggg ccgaacccga
    12961 agtagctcgg caattcttcg agctgctctt tcgtcagctc ttgctgattc ccgggggccg
    13021 cgagaaaaag aaaggcgatt gtgactgccg aaatgagcat tgattttccc gttctgtttc
    13081 aaagccggcc ggcagggcga cacctggcgg acgaaattca cctgatcagg ccgagaatgc
    13141 gttcataagt agctgccgcg tcatagcgcg gatttacgtc cggtcgggcc gaaccggcgc
    13201 ggccggagcc gaggacggag ccgtggacgg agccggcgtg ctcggttgcg aagccggtgc
    13261 gcccagatat ccgcgtatgg gtgacgacca tatcggctga ccggccgcct caccgggctg
    13321 tgtcgccggc ccggtcgtcg gagttcgctt ctgggttttg tcgatcaatc tcgcgcccgg
    13381 cggcagctcg agcacgaaac gttgcggatc gagctctaca tcgaattgaa aatcgacgta
    13441 gtcgatcgtt tcgagcttga cgcccgccgc gtcaaattcc tcctgtctcc gcagtgcccc
    13501 ggagtcctgg aggaagtaca gcaccgtccg tagcgttttg gtcttcgggt tgggttcgct
    13561 cctgacaccc tcgattaccc agaccttctg gtcgtcgatc gtctgttcgg gcagcgcttt
    13621 gagctcggcc gactggccca gaaattcaat taccgtctgc ggcacgaccg acatgccggg
    13681 gttgaccggc accttggtgt agaatttctg gtcgccgctg ccgcgaatga ggtactcgct
    13741 ttcgccgtcg agaatgatgg tggcgtcgta cggcataaag gccgattcct ggggatcagc
    13801 cgcggtccgc gtcatttccg tgtgcacctc cttgcgctgc acgtacagcg agcccttgcg
    13861 caacacctcc acgatgcctt cgccgcgtcc ctcgtgcacg gcgtcaccct gagcgcggtg
    13921 cgattccatg cgcatgcggg ccgtcatgga actgtgacgc ccccaagcct cctgtattgc
    13981 cttttgcacg gcctccaagt cactggcctg agcgctcgac gcgaagaaag ccgccattcc
    14041 ggaaaacagg acaaaaaacg tgcgactcat gcctggcaga ctccatactc gctggccggc
    14101 gcagcactgg gtcagacaac cgacgccgcc ggctgattgg tcgaaattgt aagggcgaca
    14161 aaaacggccc gctacccgcc cgcaagcagc cggtcctcca aagcgcccgc ccccgcccgc
    14221 cgacggccaa gtccggcaag ccttgccggg tcctcacagg ccgcaatatc gccccgcggc
    14281 ttgcccccca ccgcttgcga gctatgggtc gggcggtaga attctgcgtg cgcggtattc
    14341 atcgatgttg tgttgctgca tcgcgcgacc gcaaggaggc gtagatgggc aagataagag
    14401 tcgctattgc gggtgttggc aactgcgcca gttcgctcct gcaaggcatt gagtactaca
    14461 agcattcgcg aagcaacgag accgccggct tggttcaccc caagatcggc ggctatgaaa
    14521 tcggcgacct ccaaccggtt gcggcctttg acatcgaccg ccgcaaggtc ggacagccgc
    14581 tggaaaaggc cgtgttcgcg ccgccgaact gcaccacggt cttccagcct gagcttccgg
    14641 cctacggcgt gacggttcag
//
LOCUS       DAOSYS010000067        14589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3484_length_14589_cov_5.642975, whole genome shotgun sequence.
ACCESSION   DAOSYS010000067 DAOSYS010000000
VERSION     DAOSYS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14589
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3484_length_14589_cov_5.642975"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLU99_05830"
     CDS             <1..197
                     /locus_tag="PLU99_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16613.1"
                     /translation="AGLSPILRADDYRTFNDPRSLALALQDYRRDPGRTLARAGKTAQ
                     QLSARHSYTQRLLQLAELLP"
     gene            194..3151
                     /locus_tag="PLU99_05835"
     CDS             194..3151
                     /locus_tag="PLU99_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16614.1"
                     /translation="MKRPCLLRQAPRALFCVTAILFLFAAAGFAADDVPVVTTIADFE
                     DDSVATTIASVENVLRTACALKRSTVPARGQSCLQLEIGVTGGEASAVVDFRLRDPGR
                     YDQADRIAAFYWLNGGSFDLAFRLRDANDQIFETPAQTVSAKNRWTYVEIACDPDKLK
                     RIQGTGELRWPVQFQGFRASTSKLGKQTLYLDEFQIGHQVERRAVVHGEFVFDEPTRI
                     FEPGATVKTALALENCSRTEKLQVSVELAWIRSDGSVLKTQSGSKSLPPSRPGFRSRQ
                     AIDFSQRIEQPGLYRLAARARAGGWLAPAVFQTSIAVTPRNTAISRGRSVFFGVRSGL
                     VYEPVADQRLEIAIARDIGAHLLAIETPWRQIEPAQGKFDFSALDPAVNELVAKNIAP
                     LIVLTDPPDWAPEGAARIDAVAAVFCRLAEKYGAAVSLFQPEHQVLGTRSLAELVPAL
                     DRIQERLKKTREDAAAVSPPVPATPESAAELKANLPASKVLWCVQTAGDVHAALQALE
                     TFRNAAGVSWDTKFWWQHHAAPMVGPTTFVDAEDVLKHYVHAALAGVRGLIWYDLRDD
                     DNDRGQPNALRGLVRRDFSPKASLLGYAAAAGMLTGLACRGPVDGTPPEFDSALFVGS
                     DRHLAVLLPKPNRIRPALLAVLQGVPGKLQLQDFERRSFKPLTGLPTSLAPTLNRPFF
                     ISLPLTTPQPEPQLAFDRCPISMPATLFCGESGHNILEFTPPEDVRRGSIQLAAPRGS
                     QITLNSDRQWFKAKQGETQQLRLEITRPAEKKLETVKAELKLMLDKAVLDIPVDVCGL
                     VNAPPADTNWPRQQDAFRLGELAPAAGQGASAGVTVFGAYTRQSFELLIVLRDERLVP
                     LRVLADGTTIGDDLLLGVAGDHADEHTEVRIDLASDPPQLRPVHGTTGGELAAWTCRA
                     LPGADGERVYHVQIPSRAFGAAALSAGSKLRLAVRYADDDSDGLSPVALQLGRGLDGS
                     RSSEEFLWVRLAE"
     gene            complement(3687..4100)
                     /locus_tag="PLU99_05840"
     CDS             complement(3687..4100)
                     /locus_tag="PLU99_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16615.1"
                     /translation="MLRRPEQAEIIQDLLRERDRAGVILPQDPQAGQSSGEIHAATPD
                     ASGLIPEGTILVERPGRLVSDGGRPMFVFLTSATGGRPQSMELLPCQLLEALERESAR
                     SSEFTVSGEVTRYRGRNYLLLLKVLRRVSHGNLSP"
     gene            4340..5029
                     /locus_tag="PLU99_05845"
     CDS             4340..5029
                     /locus_tag="PLU99_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOO16616.1"
                     /translation="MKQRDADVVIFGAGGLGRMVQDILRQSGRWRPAAYLDSDPRKHG
                     KLVGGLPVMGDMRQAAQLLRDGLTRAVVAIGHNQTRVAIASQLAARGFQLVSAIHPLV
                     SISPTALVGDHVIIGPRVSICVHARIGAHTVVSAGSIIEHDNFIGRGVFLHPAVRLAG
                     TVIVEDLATLGIGACVIPGRRIGRGALVQPGAVIIADVAPGAMVAGVPGNERSASRFV
                     SESIPGQLLTT"
     gene            complement(5148..7070)
                     /gene="pepF"
                     /locus_tag="PLU99_05850"
     CDS             complement(5148..7070)
                     /gene="pepF"
                     /locus_tag="PLU99_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOO16617.1"
                     /translation="MNRRNTWNGVWLMAAATLWAATVLNDHSTALAGEPAEEVRKVPE
                     RTEIDQKYRWDLESTFKSVADWEQEYAQVDEAVKQVASKKGTLAAGPTELLTLLKLTD
                     DTEARLDRVAVYAHLMADQDTRVNENQALLERAMDLASRYRQATAWIEPELIKIPQET
                     INAWMDQDKELAVYRHSFDNLWRMKKYILSEAEENLIAKAGRVFSTPRSTYDRLTLAD
                     IQFPTITDEQGQKFELNDAVFYKCVRSPDRRMRKEGYEGIVGTYSKYRNTMAALLNGE
                     VQTHLFRVRARGYDSCLDAALTPGNIPAEVYNNLVCTVNENLPLLHRYTTLRKQALKL
                     ADGVHDYDLYCPFTEEQKLDYSYEDGVKLILEALAPLGPEYLTPMKKGFESRWVDVYP
                     TKGKHSGAYSSGTYLTQPYILMNFYGAYDDVSTLAHEMGHSLHSFFSRGTQPYVYSNY
                     DIFCAEVASTTNEILLQNHVLKQVKDPQTRLYLLVEFLETFRGTVFRQTMFSEFEQKI
                     HELAEAGVPLTADELGKTYGTIMKKYYGPDYVHDDLVDDYWIRIPHFYRNFYVYKYST
                     SFCAASNIARRIVEGQPGAVEAYLTFLKSGSSKYPIELLQAAGVDMTTPEPILDAMQH
                     FKNLLDETEKLLTEVK"
     gene            complement(7484..7789)
                     /locus_tag="PLU99_05855"
     CDS             complement(7484..7789)
                     /locus_tag="PLU99_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HOO16618.1"
                     /translation="MPRAICKGQVIAESERTIVLDGIHYFPQGAVRSELLRTSGTRRW
                     CPDQGEARYYHLLIGDTLQRDAAWYFPNPAPSAGRLAGYVAFGGEVEVVQSEMLALV"
     assembly_gap    7953..8052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8098..8499
                     /locus_tag="PLU99_05860"
     CDS             8098..8499
                     /locus_tag="PLU99_05860"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO16619.1"
                     /translation="MYYFTPAAALPRSHVERIHPAGSLIGKRVIDPDGQVLGTIQEIM
                     LDIEARRIVYAVVSFADALGLRDRLYAVPWQALKLDDECECFVLDISRQRLEQAPGFD
                     KHNWPDMSNEIWGRRVHNFYGVRPYWEELED"
     gene            complement(8640..9710)
                     /locus_tag="PLU99_05865"
     CDS             complement(8640..9710)
                     /locus_tag="PLU99_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOO16620.1"
                     /translation="MSISVRNVSKTFGTFQALRDVSLDVQTGELVALLGPSGSGKTTL
                     LRIIAGLETPDCGTVYFHGEEATNRRVQERRVGFVFQHYALFRHMTVFENVAFGLRVR
                     PRRLRPRRSEIRERVQKLLELVQLDWLADRYPSQLSGGQRQRVALARALAVEPKVLLL
                     DEPFGALDAKVRQELRRWLRRLHNEINVTGVLVTHDQEEALEVADRVVVMNAGKIEQV
                     GTPEEVFHNPASEFVLNFLGHVNVFHGRAEGTKAIFGSLKVDYPNGANNVNNGSLTGS
                     LGVRGFVRPHDLEIAVEPSENTLLAATIVRIHAAGPQAKIELISELGESIQVETSQER
                     YRQLGLAAGQRVYVCPRQITIF"
     gene            complement(9707..10603)
                     /gene="cysW"
                     /locus_tag="PLU99_05870"
     CDS             complement(9707..10603)
                     /gene="cysW"
                     /locus_tag="PLU99_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOO16621.1"
                     /translation="MSATVAIQSKPKRSAEPLTEPAPVRWLLIGVAGLFLALFLFLPL
                     AAVFVQALEHGVATYFAALREPDARAAIRLTLLTAAIVVPLNLLFGIAAAWAIAKFEF
                     PGKSTLTTLIDLPFAVSPVIAGLIYVLLFGRRGYFGPWLDAHNIHIIFALPGIVLATL
                     FVTFPLVARELIPLMQTQGTQEEEAAAVLGARGWQTFFRVTLPNIKWGLLYGVILCNA
                     RAMGEFGAVSVVSGHIRGLTNTMTLHVEILYNEYNFAAAFAIASLLTLLALLTLGAKS
                     LVEWRTRRMAPATDEPKDELAS"
     gene            complement(10606..11496)
                     /gene="cysT"
                     /locus_tag="PLU99_05875"
     CDS             complement(10606..11496)
                     /gene="cysT"
                     /locus_tag="PLU99_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOO16622.1"
                     /translation="MTSSTHRLAGASAVAIAAPARRHRSVLPGFRLSLGFALLYLSLI
                     VLIPLAGLALKATQMSASQFWAAVSSPRAIASYKLSFGAAFVAAAINAVFGALVAWVL
                     VRYRFPFRKLIDALVDLPFALPTAVAGLALTSIYVSNGWIGRFLEPLGLQVAFKPLGI
                     IVALTFIGLPFVVRTVQPVLQDLDHEVEEAAASLGARRWHTLVKLILPEIWPALLTGF
                     ALAFARAIGEYGSVVFIAGNMPMKTEITPLLIVIKLEQYDYAGATALAVVMLGASFVL
                     LLAINLLQRWSARRQAGCTD"
     gene            complement(11522..12634)
                     /locus_tag="PLU99_05880"
     CDS             complement(11522..12634)
                     /locus_tag="PLU99_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOO16623.1"
                     /translation="MTTTASKTNAANQAAYARKSSCKLSVTREVGPLLGLALIGFSLL
                     VGNDYAAAQQAASAKQGDVTLLNVSYDPTRELYQDFNAAFIQHWKEKTGATVTIRQSH
                     GGSGKQARSVIDGLEADVVTLAMAYDIDAIHEKGQLLPKDWQKRLPDNSTPYTSTIVF
                     VVRKGNPKGIKDWDDLVKPGISVITPNPKTSGGACWNYLAAWGYARQKYGGDEQAREF
                     VTKLFKNVPVLDSGARGSTTTFLQRGMGDVFLAWENEALLALNEIGKGKVELVYPSIS
                     ILAEPSVAVVDHFVDKHGTREVATAYLEYLYSEEGQEIVAKHYYRPRMAKVAAKYAER
                     FPQMKMLTIDGDFGGWHAAHREHFADGGVFDSIYQQ"
     gene            complement(12716..12955)
                     /locus_tag="PLU99_05885"
     CDS             complement(12716..12955)
                     /locus_tag="PLU99_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011899861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOO16624.1"
                     /translation="MAPRKRDPHRMVTMKIPGELYDNLGKLIEGTGFRSVTEFATYVL
                     RDVAAGGKLDRKPPGLSDAEVEAVRARLRALGYIP"
     gene            13438..13809
                     /locus_tag="PLU99_05890"
     CDS             13438..13809
                     /locus_tag="PLU99_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOO16625.1"
                     /translation="MAIEPAVPDTAVARAGKVTSLDVGLFLIRVLPGIVFLFHGSQKL
                     FAWFGGPGLGTFGEFLAARGVPAPLLAATLATLAEFVGGLSLLTGIGLRLMAIPLFVT
                     MMVACFLVHPQAFSGEHDGME"
     gene            14055..>14589
                     /gene="tsaB"
                     /locus_tag="PLU99_05895"
     CDS             14055..>14589
                     /gene="tsaB"
                     /locus_tag="PLU99_05895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOO16626.1"
                     /translation="MSSPLLLAVETSQAQGSVALAAAGRIVASRGLSAERRHASELLP
                     AIAELLRETGHKPADIATFAYSAGPGSFTGLRVAATLGQMLQSALGCGVVAVPTLEVI
                     ARNALGYVPESAAGTFPPARAPQRVAVILDARGGRIYAAMFELRESQVDEIAVLQPAG
                     AFEPAAWFAQLGASVWVM"
BASE COUNT         2529 a         4564 c         4591 g         2805 t
ORIGIN      
        1 cggccgggct gagcccgata ctccgcgcgg acgattaccg caccttcaac gacccgcgca
       61 gcctcgcgct ggccttgcag gactaccggc gcgacccggg gcgcaccttg gcccgggccg
      121 gcaagaccgc ccagcaactt tccgcgcggc actcgtacac gcaacgcctt ctgcagttgg
      181 ccgaactgct gccgtgaagc gcccttgcct cttgcggcaa gccccgcgcg cgctgttctg
      241 cgtgacggcc atactgttcc tgttcgccgc agccggcttc gcggccgacg acgtgccagt
      301 cgtcacgacc atcgccgatt tcgaggacga ttcggtcgcc accaccatcg ccagcgtgga
      361 gaacgtgctg cgcaccgcgt gcgcactcaa gcgatccact gttcccgcac gcgggcagag
      421 ctgcttgcag cttgagattg gcgtcaccgg cggcgaggcc tcggccgtcg ttgatttccg
      481 cctccgcgac ccaggccgct acgatcaggc ggaccgaatc gcggcgtttt actggctcaa
      541 cggcggatca ttcgatctgg cttttcgatt acgcgatgcg aacgatcaga tcttcgaaac
      601 gccggcccaa accgtgtccg ccaagaatcg ctggacctac gtcgagatcg cctgcgatcc
      661 tgataagctc aagcgtattc aggggaccgg cgagctgaga tggcccgtgc agttccaggg
      721 ttttcgcgcc agcacgagca aactcggcaa gcagacgctc tacttggacg aattccagat
      781 cgggcatcaa gtcgaacgcc gcgccgtggt gcacggcgag ttcgtcttcg atgagccgac
      841 gcgcattttc gaacccggcg caaccgtcaa aacggcgttg gccctggaga actgctcccg
      901 aaccgagaag ctgcaagtca gcgtggagct ggcctggatc cggtctgacg gctccgtgct
      961 caagacgcaa tccggttcga aaagtctgcc gcccagcagg cccggttttc gctcgcgcca
     1021 ggcgatcgat ttctctcagc gcatagaaca gccaggcctg taccgcctgg ccgcgcgggc
     1081 ccgcgccggc ggctggctgg cccccgccgt gttccagacg tccattgccg tcacgccgcg
     1141 caataccgca atctcgcgcg ggcggtccgt gttcttcggc gtgcgctccg gcctagttta
     1201 cgaaccggtc gccgatcagc ggctcgaaat cgcaatcgca cgcgacatcg gcgcacacct
     1261 gctcgccatc gaaacgccct ggcggcagat tgaacccgca cagggaaagt ttgatttctc
     1321 cgcgctcgat ccggccgtca acgagctggt tgccaagaac atcgcaccgc tgatcgtctt
     1381 gactgacccg ccagactggg ccccggaagg cgctgcccgc attgacgcag tggccgcggt
     1441 attctgcaga ttggccgaaa agtacggggc agcggtgtcg ctgtttcaac ccgagcacca
     1501 ggttctgggc acgcgcagcc tagccgagct ggtcccggcg ctcgaccgca ttcaggagcg
     1561 cttgaagaag actcgcgaag atgcggcggc ggtctctccg cccgtgcctg cgacgcccga
     1621 gtctgccgcc gagctcaagg ccaacttgcc ggcctccaag gtgttatggt gcgtccagac
     1681 ggccggtgac gtgcacgctg cgctgcaagc gctcgaaacg ttccgcaacg ctgcgggcgt
     1741 cagttgggac accaagttct ggtggcagca ccacgccgct cccatggttg gtccgacaac
     1801 cttcgttgac gccgaagacg tattgaagca ttacgtacat gcagcgctgg cgggcgtgcg
     1861 cgggctcatc tggtacgacc tgcgcgacga cgacaacgat cgcggtcagc caaacgcgct
     1921 gcgcgggctg gtgcgccgag atttcagccc caaggccagc ctgctgggct acgccgcggc
     1981 ggccggcatg ctcaccggcc tggcttgccg cggcccggtg gatggaactc cgcctgagtt
     2041 cgattccgcg ctgttcgtcg ggagcgatcg ccatctggcc gtcctgcttc cgaagcccaa
     2101 tcgcattcgg ccggcgcttc tggcagttct ccagggcgtg cctgggaaac tccagttgca
     2161 ggatttcgaa cgacgttcgt tcaagccact gaccggcctg cccacgtcgc ttgcgcctac
     2221 cttaaaccgc ccgtttttca tctcgctccc gctcacgacc ccccaacccg aaccgcaact
     2281 ggccttcgac cgctgcccaa tcagcatgcc ggctaccctt ttctgcggag agagcggcca
     2341 caatattctg gaattcacgc cgccggagga tgtgcggcgt ggttccatcc agttggcggc
     2401 gccgcgaggt tcgcagatca cgctcaattc ggaccgccag tggttcaaag ccaagcaggg
     2461 cgagacccaa cagctccgcc tcgagatcac ccgacccgca gaaaaaaagc tcgagacggt
     2521 aaaagccgag ctgaaattga tgctcgacaa ggctgtgctg gatattccgg tcgatgtctg
     2581 cggccttgta aacgcaccgc ccgcggatac caactggccg cgccaacagg acgcgttccg
     2641 cctgggagag ctcgcgccgg ccgctggtca gggagcgagc gcgggcgtca cagtcttcgg
     2701 ggcctacact cgccagagtt tcgagctgct gatcgtgcta cgcgatgagc ggctggtccc
     2761 gctacgggtc ttggccgacg ggaccaccat cggcgatgac ctgttgctgg gtgtggccgg
     2821 cgatcacgcc gacgagcaca cggaagtgcg catcgacctt gcgtctgatc cgccccaact
     2881 ccggcccgtt cacgggacca ccggaggtga gctggcggct tggacgtgca gggcgctgcc
     2941 gggcgcggat ggcgagcgtg tgtaccacgt tcagataccc agccgggcat ttggggcggc
     3001 tgccttgtcg gcgggctcaa aactgcgttt ggcggttcgc tacgcggacg acgatagtga
     3061 cggattgtcg cccgtcgcgc tgcaactggg gcgcggcttg gatggttcgc gatcgagcga
     3121 ggaattcctg tgggttcggc tggcggaatg agctaagagg ccaatgggtg ggctgccggt
     3181 caacgtcctt caggagtggg gttggggttt tgagcgaggg ggccatcaag ttccgaatcg
     3241 gatagaatga gtgcctcttg atggctaaag acgagtgaca cggtcagtcc acccatagcc
     3301 gtcgaaaact acctctgccg gagagaaaga acaccggcaa agattccatt ctctgccgat
     3361 gcgtttcggt cgttttgctg ctcatccctg agctgcttgt tcattgcgac cgggatccac
     3421 attaatagtc cgtcatgttc gtccggaatt aaagataaca cgacgcggat tgttgggcat
     3481 caggaatttt gcccattttt acaagggcgt gttaaaagag cgcgagtcgt ttgttgacag
     3541 agttgacagg ccacaggatg atggcccggc gctttctttc ggcacaagat attggggcgg
     3601 ttcgcaatat tagctaccgc ttaaacgcgt cccaaattcg aaaaaaaacc ccgcaaattc
     3661 acgatgcgcg ggatttcgcc cccatttcag ggcgataagt tgccgtgact gacccggcgc
     3721 agaaccttca gcagcagtaa ataattgcgc ccccgataac gtgttacttc gcccgacacc
     3781 gtgaattccg agctcctggc cgattcacgc tccagcgcct ccaaaagctg gcagggcagt
     3841 agttccatgc tttgcggccg cccgccagtg gccgacgtca gaaaaacgaa catcggtcgc
     3901 ccgccatccg acaccagccg cccgggccgc tccaccagaa tcgtgccttc gggaatcagg
     3961 ccggaagcgt ccggcgtcgc cgcatgaatc tcacccgagg attggcccgc ctgcggatcc
     4021 tgaggcagga tcacgcccgc cctgtcgcgc tcgcgcaaaa gatcctggat gatctctgcc
     4081 tgctcaggac ggcgcagcat gcgcgggccg gccggcgccg aagccggtcc agggccgcct
     4141 tcggccgatc ccaaggcccg ctgagccata cttttcagaa acgtcgcgcc ggccgcgacc
     4201 agcgaaagct ttagcagtgt ccgtctttcc atcgggccac ctcgtcacgc agcaagcaga
     4261 gccgccacac gccttccatc gtaccacatt ccgccgctga tatcgccagc cccgccggcc
     4321 gctgccgata agtccggatg tgaagcagcg tgacgccgac gtcgtcattt tcggtgcggg
     4381 cggcctgggc cgcatggtgc aggacatcct gcggcagtcc gggcgctggc ggccggccgc
     4441 gtacttggat tccgatccgc gcaagcacgg caagctggtg gggggcctgc cggttatggg
     4501 cgatatgcga caggccgcgc aattactgcg ggacggtctg acgcgggccg tggtggctat
     4561 cggccacaat cagacacggg tcgccatcgc cagccaactt gcggcgcgcg gcttccagct
     4621 cgtgtccgcc attcacccgc tggtcagcat ttcgccgacc gcgctcgtgg gcgatcacgt
     4681 gatcatcggt ccgcgcgtca gcatctgcgt acacgcccgg atcggtgcgc acacggtcgt
     4741 ctccgcgggg tcgatcatcg agcacgataa cttcatcgga cgcggagtgt tcctgcatcc
     4801 ggccgttcgg ctagccggaa cggtcattgt tgaggacttg gccacgctgg ggatcggggc
     4861 ctgcgttatt ccgggccggc gcatcgggcg tggggcgctg gttcagccag gagcggtcat
     4921 cattgccgat gtggcgccag gggcaatggt agcgggggtg cccgggaatg agcgatccgc
     4981 ttcgcgtttc gtcagcgaat cgattccagg ccaactgctg acgacataaa accggaaggc
     5041 ctcgcagcat gaaaaaacag gggcctggcc gcgcgactcg cagccaagcc cgctcgatct
     5101 ctagatctct tgatccctag atccctagat ccctagatcc cttctgctta cttcacctca
     5161 gtcagcagct tctcagtttc atccagcagg ttcttaaagt gctgcatggc gtccaggatc
     5221 ggctcgggcg tggtcatatc cacgccggca gcctgcagca gctcgatcgg gtacttgctg
     5281 gaaccagact tgaggaatgt caaataggct tccaccgcac ccggctggcc ctcgacgatg
     5341 cgccgcgcga tgttgctggc cgcacagaag ctggtcgagt acttgtaaac gtagaaattc
     5401 cggtagaagt gcgggatgcg gatccaataa tcgtccacga gatcatcgtg cacgtagtcc
     5461 ggcccgtagt acttcttcat gatcgtgccg taggtcttgc ccagttcgtc agcggtcagc
     5521 ggcacgcctg cctcggccag ctcgtggatc ttctgctcga actcgctgaa catcgtctgc
     5581 cggaagaccg tcccgcggaa cgtctcgagg aactcgacca gcaggtaaag ccgcgtctgc
     5641 gggtccttca cctgcttgag cacgtggttc tgcaacagaa tctcgttggt cgtcgaggcc
     5701 acttcggcgc agaagatgtc gtagttcgag tagacgtacg gctgcgtgcc gcgcgagaag
     5761 aacgagtgca gcgagtggcc catctcgtgc gccagggtcg acacgtcgtc gtacgcgccg
     5821 tagaagttca tcaggatgta gggctgcgtg agatacgtgc cgctggaata agcgccgctg
     5881 tgcttgccct tggtcgggta gacgtccacc caacgcgatt caaagccctt tttcatggga
     5941 gtgaggtact cgggtcccaa cggggccagc gcttccagga tcagcttcac gccatcctca
     6001 tacgagtaat ccagcttctg ctcctcggtg aaggggcagt acaggtcgta atcgtgaacg
     6061 ccgtcggcca gcttcagggc ctgcttgcgc agcgtcgtgt agcgatgcag cagcggcaga
     6121 ttctcgttca ccgtgcacac caggttgttg taaacctcgg ccggaatgtt gccgggggtc
     6181 aacgccgcgt ccaggcagct atcgtagccg cgggcccgga cccggaagag gtgcgtttga
     6241 acctcgccgt tcagcagcgc ggccatcgtg ttgcggtact tggagtacgt gcccacgatc
     6301 ccctcgtaac cctctttgcg catgcggcga tcgggtgagc gcacgcactt gtagaacact
     6361 gcgtcgttga gctcgaactt ttgcccctgc tcgtccgtga tcgtcggaaa ttgtatgtcc
     6421 gccagcgtca gccggtcgta cgtcgagcgc ggcgtcgaga acacgcggcc ggccttggcg
     6481 atcaggttct cctcagcttc cgacaagatg tacttcttca tgcgccacag gttgtcgaaa
     6541 ctgtggcggt aaacggccag ctctttgtcc tggtccatcc aggcgttgat tgtttcttgc
     6601 ggaattttga tcaattcggg ctcgatccag gccgtggcct gccggtagcg gctggccagg
     6661 tccatggccc gttccaggag ggcctggttt tcgttgacgc gcgtgtcctg gtcagccatc
     6721 aggtgggcgt aaacggcgac ccggtccagg cgggcttccg tgtcgtcggt cagcttcagc
     6781 agagtcagca gctccgtcgg gccggccgcc aaggtgcctt tcttggaggc tacttgcttg
     6841 accgcctcgt cgacctgggc gtactcctgc tcccagtcgg ccacgctctt gaaggtgctt
     6901 tccaagtccc agcggtattt ttgatcaatc tcggtgcgct ccggcacctt gcggacttcc
     6961 tcagccggct ctcctgccag cgccgtcgag tgatcgttga gtacggttgc cgcccaaagg
     7021 gtcgcggctg ccattagcca cactccgttc caggtgttgc gtcgattcat gttgggcctc
     7081 gatgcgggaa acgcgtctca cggcggccgg gctgtcccgc gaatagctcc agccgtccgg
     7141 acaacgtaaa cgcagaatgg gattgaggca acgtgtcggg taaggcgtgc ggtcgggggc
     7201 gggggtagtt gcgcgttttg gcggtcaggc ttcgttctcg gcgttgggtg ccatacccaa
     7261 gccgcgtggc ggcgcgggca tgccctggcc gggagcctcc catctgctcc gtcgttggcg
     7321 aaggcatgcc cgcgcttaca gcttgggcat ggcacccgag ctttcgttga ggaaggcatg
     7381 cccgcgctta cagcttgggc atggcacccg gccgcctcgc aacagtctgg ctcaaactat
     7441 tgcgagcatc ccctcgctca aactattgcg agcatcccct ggctcagact agcgcgagca
     7501 tctcgctttg caccacttcg acttcgccgc cgaacgcgac atagcccgca agccggccgg
     7561 cgctgggtgc cgggtttggg aagtaccaag ccgcgtcgcg ttgaagcgtg tctccgatca
     7621 ggaggtggta gtagcgggcc tcgccctggt ccgggcacca gcggcgtgtg ccgctcgtgc
     7681 gaagcagttc gctgcgtacc gcgccttgcg gaaagtagtg aattccgtcc agcacgatcg
     7741 tgcgctcgct ctcggcaatg acctgtcctt tacagatcgc ccttggcatt ggtcttcgct
     7801 cgattgaaag ggcctcagcc gcccgccaac attaatatga tacagcgctt tgcacggaca
     7861 aatcgcgtga tttagtcgga ttacctcgat tttaggcggc gtttatggct gcgtaatacc
     7921 ggccgtatat cctgccattt gcgagtccgg ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nngttgacga acattgccct ggccgaaagt ctcgtggagt tgattagatg
     8101 tactacttta cgccagcagc ggccttgccg cgaagtcatg tagagcggat tcacccggcc
     8161 ggcagcctga tcgggaagcg cgtgatcgat ccggacggcc aggtattggg gacgatccag
     8221 gagatcatgc tggacatcga ggcccggcgc atcgtgtacg cggtcgtttc gtttgcggac
     8281 gcgctgggct tgcgcgatcg cttgtatgcg gtgccgtggc aagcgttgaa gctggatgac
     8341 gagtgcgaat gcttcgtgct ggacatctca cgccagcgcc tggagcaggc gccgggtttc
     8401 gacaagcaca attggcccga catgagcaac gagatctggg ggcggcgggt tcacaacttt
     8461 tacggggtgc ggccgtactg ggaagaactg gaggactagc aggggggcgg cgtgataaag
     8521 tgataaggtg atagagtgat aaagcgatcc cgcgggcccg tcctttatca ctctatcact
     8581 ttatcacgtt atcacgctat gggcgggcag gcccggaact gtccagcggc acacgcaggt
     8641 tagaagattg taatctgtcg tgggcagacg tagacgcgct ggccggctgc cagccctagc
     8701 tgccgatagc gctcttgcga cgtctcgact tgaatcgact cgcccaactc gctgattagc
     8761 tcgatcttgg cctgcgggcc ggcggcgtgg atgcgcacga tggttgcagc cagtagcgtg
     8821 ttctcgctcg gttcaaccgc gatttccagg tcgtgcggcc gaacgaagcc gcgcacgccc
     8881 agcgagccag tcagcgaccc attattgacg ttgttcgcgc cgttgggata gtcaaccttc
     8941 agcgagccga aaatggcctt ggtgccttcg gcgcggccgt gaaagacatt cacgtgcccc
     9001 aggaagttca agacgaactc gctggccggg ttgtggaaga cttcctcggg cgtgcccact
     9061 tgttcgattt tgccggcgtt catcaccacg acgcgatcgg cgacctccag agcctcttcc
     9121 tgatcgtgcg tgaccaatac gccggtgacg ttgatctcgt tgtgcaggcg ccgtagccag
     9181 cggcgcagct cctgccgcac tttcgcgtcg agcgcgccga aaggttcgtc gagcagcagc
     9241 accttcggct caacggccag ggcccgcgcc agtgcgacgc gttgccgctg gccgcccgaa
     9301 agctgcgagg ggtagcggtc ggccagccag tcaagctgca cgagttccag caatttctgc
     9361 acgcgctcgc gtatttcgct gcgccgcggc cgtaggcggc gggggcgcac gcgcaggccg
     9421 aaggccacgt tttcgaagac ggtcatgtgg cggaagaggg cgtagtgctg aaagacgaaa
     9481 cccacgcgcc gctcctggac gcgccggttg gtggcctcct cgccgtggaa gtagacggtg
     9541 ccgcagtccg gcgtttccag tccggcgata atccgcagca gcgtcgtttt gcccgacccg
     9601 gaagggccga gcagtgctac tagttcgccg gtctgcacgt cgaggctcac gtcccgcaga
     9661 gcctggaacg tgccgaaagt cttcgatacg tttcggacgc tgatgctcat gatgccaact
     9721 cgtcctttgg ctcgtcggtc gcgggcgcca tgcgccgcgt gcgccattcg accaggctct
     9781 tggcccccaa tgtcagcagc gccagcagcg tcagcagcga cgcgatcgcg aaagccgcgg
     9841 cgaagttgta ttcgttgtac aggatttcca cgtgtagcgt catcgtgttc gtcaggccgc
     9901 gtatgtgacc cgaaaccacc gacaccgctc cgaactcgcc catggcccgc gcgttgcaca
     9961 gaatcacgcc gtacagcaag ccccacttga tgttcggcag cgtcacgcgg aaaaaggtct
    10021 gccagccgcg ggcgcccagt acggccgcgg cttcttcctc ctgcgtgccc tgggtctgca
    10081 tcagcgggat cagctcgcgc gctaccagcg ggaacgtgac gaacagcgtc gcgagcacaa
    10141 tgcccggcag cgcgaagatg atgtggatat tgtgcgcgtc caaccacggg ccgaagtagc
    10201 cgcgccgccc aaacagcagc acgtagatca agcccgcgat cacgggcgag acggcgaacg
    10261 gcagatcaat cagcgtggtt agcgtgctct tgccggggaa ttcgaatttg gcgatcgccc
    10321 aagccgccgc gatgccgaat agcaagttca gcggtacgac tatcgcggcc gtcagcagcg
    10381 tcagccgaat tgcggcccgc gcgtccggct cgcgcagcgc ggcgaaatag gtggccaccc
    10441 cgtgctcaag ggcctgcacg aacacggccg ccagcggcag aaacaggaac agggccaaga
    10501 acaggcctgc cacgccgatc agcagccagc gcaccggcgc cggctccgtc agcggctcgg
    10561 ccgaccgttt gggtttcgac tgtatggcga cggtggcgct catggctaat cggtacaccc
    10621 ggcctgccgg cgcgcggacc agcgttgcaa cagattgata gccagcaata gcacgaagga
    10681 tgctcccagc atcacgaccg ccagcgcggt tgcgccggcg tagtcgtatt gttcaagttt
    10741 gatgacgatc agcagggggg tgatctcggt tttcatcggc atgttgccgg cgatgaagac
    10801 gaccgagccg tattcgccga tggcccgcgc gaaggccagc gcaaagccgg tcaacagcgc
    10861 cggccagatt tcggggagaa tcagcttcac gagcgtatgc cagcgccgcg cgccgagact
    10921 ggccgccgct tcctccactt cgtgatccag gtcctgcaag accggctgca cggtgcgcac
    10981 gacgaacggc aatccgatga acgtcagcgc gacgatgatg cccagcggct tgaacgccac
    11041 ctgcaacccc agcggttcca gaaaacgccc gatccagccg ttgcttacgt aaatgcttgt
    11101 cagcgccagg ccggcgaccg cggtgggcag ggcaaacggc agatcaacga gggcgtcgat
    11161 cagttttcgg aatggaaacc gataacgcac cagcacccat gccaccaggg cgccgaagac
    11221 cgcgttaatc gcggccgcta caaacgccgc cccgaagctc agcttgtatg aggcaatggc
    11281 gcgcgggctg ctgaccgcgg cccagaactg gctggcgctc atctgcgtgg ctttcagcgc
    11341 cagcccggcc aggggaatca gcacgatcag gctcaaatac agcagcgcaa aacccagcga
    11401 cagccggaag cccggcaaga cgctgcggtg ccggcgggca ggcgctgcta tcgccactgc
    11461 gcttgccccg gccagcctgt gcgtgctcga cgtcattgcc tactgttgat cgacgcggtg
    11521 actactgttg atagatgctg tcgaacacgc cgccgtccgc gaaatgctcg cgatgcgccg
    11581 cgtgccagcc gccgaagtcg ccatcgatcg tcagcatctt catttgcggg aagcgctcgg
    11641 catacttggc cgcgactttt gccatacgcg ggcggtagta gtgcttggcc acgatttcct
    11701 ggccctcttc cgagtacaga tactcgaggt aagcggtggc gacttcacgc gtgccgtgct
    11761 tgtccacgaa gtgatcgacg accgcaacgg agggttcggc caatatgctg atcgaggggt
    11821 acaccagttc aaccttgcct ttgccgattt cattcaaggc cagcagcgct tcgttttccc
    11881 aagccaggaa cacgtcgccc atgccgcgct gcaggaaagt agtggttgag ccgcgcgccc
    11941 cggaatccag caccgggacg ttcttgaaca gcttggtcac gaactcgcgg gcctgctcgt
    12001 cgccgccgta tttctggcgc gcgtagcccc aggccgccag gtaattccag cacgccccgc
    12061 ccgaggtttt cgggttgggc gtgatcacgc tgatgccggg cttgaccagg tcgtcccagt
    12121 ccttgatgcc cttggggttg cccttgcgga ccacaaacac gatcgtcgag gtgtacggcg
    12181 tgctgttatc cggcagacgc ttctgccaat ctttcggcag caactgcccc ttttcgtgaa
    12241 tggcgtcgat gtcatacgcc atcgccaggg tcacgacgtc ggcctccagc ccgtcgatga
    12301 cggagcgggc ctgtttgccc gagccgccgt gcgactgtcg aatggtgacg gtagcgcccg
    12361 tcttctcttt ccagtgctgg atgaaagccg cgttgaagtc ctggtacagc tcgcgcgtgg
    12421 ggtcgtaaga aacattgagc agcgtcacgt cgccctgttt ggccgaagcg gcctgctgcg
    12481 ccgccgcgta atcattgcca actagcagcg aaaagccgat cagcgccaac cccaaaagcg
    12541 gcccgacttc gcgtgtcacg ctcaacttgc agctactttt ccgcgcgtac gcggcctggt
    12601 ttgccgcgtt ggtcttgctt gcggttgtgg tcatgcttca cctcgtttgt tccgtttttt
    12661 ggctatgcaa gcgcacagta tctccggccg gcgcctggcg ccgctttttg caccactacg
    12721 ggatgtaccc caaagcgcgc agccgcgccc ggacggcttc cacttcggcg tcactgaggc
    12781 cgggcggctt gcggtcgagt tttccaccgg cggcgacgtc ccgcaggacg taggtagcga
    12841 attcggtcac gctgcgaaac ccggtgccct cgatgagttt tccgaggttg tcgtagagtt
    12901 cgccggggat tttcatggtc accatgcggt gtggatcgcg ttttcgcggc gccattggtg
    12961 gatcactccg ttatgcctgc gctggccgct gggggctgcg tctttcaact ccggctccgc
    13021 cagaggcagg gcggtcatcg ggcgggcgcg actcgccggg tgtccgatac tcctattgga
    13081 gtgtaacggc actccggcga gagtgcaagc acgaatcagg ggcggtggcg cgtgtggatc
    13141 gggctgttcg taagcgctcc cgaaacagtt tcctttcgac ggcattcccc cgggcgcggc
    13201 gtgggcgact tcgcagtcca agggaccgca gacgccgcgc tggcgcattg ccccgagcag
    13261 atgcgtcccc gtttcgcatt tcgcttcgca atcgcatttg aaaacaaggg caatatgatc
    13321 gtgacggtat ttgcgtcggc gggcgagcgc tatctctcga caccgctgtt ccagttgatt
    13381 aaccacgccg ggcaccgcgg gccgaaccgc gggcgcggcc ggaaaatgga ggcttgaatg
    13441 gcgattgaac cagcggtgcc ggatacagcg gttgcacggg ccggcaaagt aacatccttg
    13501 gacgttggac tgtttctgat ccgcgtgctg ccgggaatcg ttttcctctt tcacggcagt
    13561 cagaagctgt ttgcatggtt cggcggccct ggtttgggaa cgttcggcga gttcctggca
    13621 gcgaggggcg tccccgcacc gctgctggcg gcaaccctgg cgacgctagc cgagttcgtg
    13681 ggcgggctgt cgctgttgac cggaatcggg ctgcgcctga tggcgatccc gctgttcgtc
    13741 accatgatgg tggcgtgctt cctcgtgcac ccgcaggcat tctctggcga gcatgacggg
    13801 atggaataat ccactcacgc tagcgtttat cgtcctagga ctggggctga ctggtccggg
    13861 gcgggttacg ctgcccgcgg taattcgaat ggccatgggc cgcaggggcg gtgtgaaacc
    13921 gggatgcagc tgcgtccccg tctctaagcc tgttggcgat gtgcggggat gattcttggg
    13981 gcgatgtccg ggcggaatgt gcggggcggc ttccggcatg atctgcgggg cgagggccgg
    14041 gtataatccg cggtgtgtcg tcgccgcttt tgcttgcagt tgaaacttct caggcccagg
    14101 gcagtgtcgc cctggcggcc gcggggcgga ttgttgccag ccgcgggctt tcggccgagc
    14161 ggcggcacgc gagcgaactg ctgccggcca ttgccgaatt gctgcgggag accggtcaca
    14221 agccggccga catcgccacg ttcgcctatt ccgccggccc cggcagcttc accgggctgc
    14281 gcgtggcggc cacgctgggg cagatgctgc aatcggcgct cggctgcggc gtggtcgcgg
    14341 tgccgacgct ggaagtgatc gcccgtaacg ccctcggcta cgtgcccgaa tccgcggccg
    14401 gcacgtttcc gccggctcgg gctccgcagc gcgtggcggt gatccttgac gcacgcggcg
    14461 ggcgaatcta cgcggcaatg ttcgagctgc gcgaatcaca ggtggacgag atcgcggtgc
    14521 tccaaccggc cggggcgttc gagcctgccg cctggttcgc gcaactcggc gcatccgttt
    14581 gggtcatgg
//
LOCUS       DAOSYS010000068        14565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3491_length_14565_cov_5.946175, whole genome shotgun sequence.
ACCESSION   DAOSYS010000068 DAOSYS010000000
VERSION     DAOSYS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_14565_cov_5.946175"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1340)
                     /locus_tag="PLU99_05900"
     CDS             complement(24..1340)
                     /locus_tag="PLU99_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO16627.1"
                     /translation="MKRHDSTPHATGVTRRQFVKASAAAVAGAMLTREMLAPAAYAAG
                     SDTLKVGLIGCGGRGTGAAKEALAADPGAVLTAMGDVFNDRLQNSLQTLKEAQPERVQ
                     VDPDRRFVGFDAYQKVIDSGVDVVLLAAPPHFRPAHLKAAVLAGKHVFCEKPMAVDAP
                     GVRSILASAEEASKRNLSLLAGFCWRYSDPERATFQRIIDGALGRIVSAHTAYHASPL
                     QQFPRQPGWTDMEWQLRNWFHFTWLSGDHIVEQACHSIDKIAWALGGRLPVKATALGG
                     RQARSGAESGNVYDHFSVIYEYDDGLRCFHTCRQMADCPFDNTDYFVGTEAVCRIDGW
                     KPRHEIRSHAGELKWSYDGQRRGMYQNEHDALFAAIRSGRPINDGQWMAHSTLMAIMG
                     RMAAYTGQTITWEQALNSEEKLGPPTYDFGALPVEPVAVPGQTKFV"
     gene            complement(1554..3599)
                     /gene="fusA"
                     /locus_tag="PLU99_05905"
     CDS             complement(1554..3599)
                     /gene="fusA"
                     /locus_tag="PLU99_05905"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO16628.1"
                     /translation="MAAFQISDIRNVALVGHSAAGKTTLGEAILHKAGVTTRMGSIDD
                     GTSMLDHDDESRERKHSVDSAAFYVEYEGKLLNFIDTPGMPDYIGPAIAALAGVDLAC
                     VVVSAASGIGVNTRRMFNAAGEYGLPRMIVITRIDAENANLDEILRGVRETFGHHCHP
                     INLPADGGKKLIDLLREEQGTSDLLDVAAAHTSLVDAIVETDEALMESYMETGSASLE
                     QLVPAIRKAVASGGLIPVLFVNAKAGVGIPELLEALLRCAPSPAEGKRRSVVMGEGEA
                     AKKTEVEPDRAGDFVAQVFKITSDPRSNIKYSVARVHRGVLKPDGQIFTPGERKGQRP
                     GHFFKLRGDQHAEIESAEAGDIIAFAKLDLKIGATLFAKAEDGEIPMPKFPTPMFALA
                     VEPKSRADVEKISGALHRFAEEDPCFQYFRDQETNELVMQGMGDQHLNVIRSKMRRYF
                     KLEVETKAPKIPYRETISGAVKYVEYTHKKQTGGAGQFARVFIDMEPNERGKGYEFID
                     KIFGGVIDQAFRPSVDKGVRDQMRKGVIAGFPVVDVKVSLVDGKTHPVDSKDIAFQIA
                     GRQVFKKAFVQCKPILLEPIVNIEITVPAEFVGDITRDWAGKRGQIVGQDMLPGNQIV
                     IRGTVPLAEVATYSSQLKSATGGQGSYMMEFSHYDIVPPNIQQQIVAQHKAAAEEEE"
     assembly_gap    3605..3704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3705..4912)
                     /locus_tag="PLU99_05910"
     CDS             complement(<3705..4912)
                     /locus_tag="PLU99_05910"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOO16629.1"
                     /translation="MPVVFRIPVVNIDVPGYGLVMMLGFLLSVVWAVRRATRSGGNPD
                     VVLNCAFIALIGGVGGARLMYVVHYWDEQFAPLGNWVAILLRATLGVRDGGLEVYGGF
                     IAATLGVVIYMLFWGHSFRWYLDIMAPSAALGMGLGRLGCFLNGCCWGLPSDLPWAVR
                     FPFGSNALIQQWQSGQLDLPKELLWFPPNGVLTDGRPAAPLPREMLRMTEAEFAKITS
                     AAAAIGQRQKELTARRAQATSAAEQAELDREENRLRAELGSLASSLGGGCASGAAHAE
                     VLLAMQASGLSLTQLQELARQYPALPVHPTQLYSALTLILLALLLNALYWRRTRDGQV
                     ICTMLLIEPWTRYILETIRADNPVDTFQFTISQFIALMISLIGLAGLIGLQYAKPRSP
                     RAKLWEPPPA"
     gene            complement(5000..5860)
                     /gene="panC"
                     /locus_tag="PLU99_05915"
     CDS             complement(5000..5860)
                     /gene="panC"
                     /locus_tag="PLU99_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOO16630.1"
                     /translation="MQVTRNIADTRSAVAEARRSGQRIGFVPTMGALHRGHASLIDAA
                     RRDGTYTVVSIFVNPLQFGPNEDFQKYPRDEAADLQVCQQAGVDLVFIPSTDELCRPD
                     AVTRVHVAKLTETLCGVSRAGHFDGVTTICTKLFNIVQPDVAYFGEKDFQQLAVVRRM
                     VRDLDMPLEIVGCPTVREENGLAISSRNMYLTPAERDQAACLYDGLIKARNRIHAGER
                     NASKITALLRDVITAAGPARIDYIRLVDSETMQPVERISGPVLVALAVHIGAARLIDS
                     FFVDPGRRGA"
     gene            5992..7728
                     /gene="ggt"
                     /locus_tag="PLU99_05920"
     CDS             5992..7728
                     /gene="ggt"
                     /locus_tag="PLU99_05920"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOO16631.1"
                     /translation="MAHQRRATVFLSLAVCLWLPGGAARAADPPWLARGRTGMVASDS
                     PEASQIGADVLAAGGNAFDAAVATSFALAVARPQSTGLGGGGFMVAWVAAEKRFVVLD
                     FRECAPQAATPERYAELAAQQGDGFSPSVYGGNAVAVPGQLAGLVEINRRFGTQPLKE
                     LIRPAAALAEAGFAVDAHYRGACKEALAVLEKSPALMLRYKVLYETLLNKGKLPQIGE
                     RLKRPDLARGLRLIAQEGPRVFYEGPVAAAIVSAVKASGGELTPEDLRNYRVLERAPI
                     RAAMGEYELVTMPPPSSGGVCLIEALNILQCLAADVGGVTKLRSKDSDLYPAALARAF
                     KHAFADRARWLGDPDFTPVPVARLTDKQYACDLALKAGQNPEDFGSRQLPEDRGTSHF
                     CVADHLGNVVALTETINGVFGSLVVAEPYGILLNNEMDDFLTVRGQANLYGLLQGEAN
                     LVGPGKRPLSSMCPTIVVQGGRPVLALGGSGGPRIITGVAQVLLNVMEFGESLDGAVE
                     AVRLHHQWQPDVVFFDGQPAAELRAALEGAGLKVSDRRRGAVVQALQILPDGTLVGVS
                     DPRRSGRPAGVP"
     gene            complement(7838..8122)
                     /locus_tag="PLU99_05925"
     CDS             complement(7838..8122)
                     /locus_tag="PLU99_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16632.1"
                     /translation="MSKARFVCLALAGLVLAAPFTCRPFAATDPYTSFIAGVGNQAIS
                     QGVSAGLANTNDTIADLLQTPLTNLYQSLWTGWVDYTFPRDPTYGTLLVQ"
     gene            8393..8575
                     /locus_tag="PLU99_05930"
     CDS             8393..8575
                     /locus_tag="PLU99_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16633.1"
                     /translation="MLELLIGQDVMFGLPRQCVTSADAKPSGMLAAAFFDLSNQDGFS
                     IALSVVAAVDSLRPVG"
     gene            8820..9377
                     /gene="amrA"
                     /locus_tag="PLU99_05935"
     CDS             8820..9377
                     /gene="amrA"
                     /locus_tag="PLU99_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOO16634.1"
                     /translation="MPIPERGPQSCWPAIARDAIEHAVRYGETAADCAAPAQHEPEFG
                     GVFVTLHKLGRLRGCMGLLEPQESLTAAVRRAAVCAALQDPRFHPVTLAELPAVEIEV
                     SVMSRPQPLRDLQQLELGRHGIIVGRGRNRGLFLPQVATEYGFSREEFLSRCCSEKAG
                     LPADAWRDPRTEVFIFTTDVFAELS"
     gene            complement(9572..10303)
                     /locus_tag="PLU99_05940"
     CDS             complement(9572..10303)
                     /locus_tag="PLU99_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16635.1"
                     /translation="MRTRCLSLGLVLALASGALADLSDISEVIFRIEATNASGTGVLE
                     FTRDQLVYNPSTSTYIWNTATQFIYDEFFTPIATLQNANMALQLDSTKKIAGAFAVQS
                     GSSDTIFTITMPQLTFATLATPSAAAGLAINVTDTNNNGVAMQALPSAGGILITHYNG
                     LVPTGTLFEEVLPGVTTPTGSTSTLSFVPYSPLPFAVSDMNSQLAFSLTAGDLGGGTH
                     YFQIIPEPAGLALLAGALLLLGRRR"
     gene            complement(10376..11191)
                     /locus_tag="PLU99_05945"
     CDS             complement(10376..11191)
                     /locus_tag="PLU99_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16636.1"
                     /translation="MASGSRLHVAVECRAQQGPSDQGKRNAMKLDRSGMRALGALVVV
                     LSFVAGSALADVSEVVLRITASSGRGTGVLEITADEGIWDGGDFFWSNTEDISLMSGD
                     QVVGTIVKDSYIAISSNPQIAMGFSFQAGDAATDVTLTSALLSFPTIANASARADAAF
                     TVMDIMGEGVSLVGLQPGGGAYRAYYNDSITFAEAIGPIDVIPPATLAEGNFAYPGGG
                     AHVPIGVASNMGAEIAFSLSAYGFVSGSSNFEIVPEPAGLLLLAVGVCVLRRR"
     gene            11448..12872
                     /locus_tag="PLU99_05950"
     CDS             11448..12872
                     /locus_tag="PLU99_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16637.1"
                     /translation="MRNLLVLGPKTIPLRVRWPLGTNAGMLACCHFLALAQAMVLPSV
                     AQGFLSRPPSAYRFEPGDRLVYELRSESTPLTPGAAGERCFGQIEIWCLALKNGEALL
                     LLDFIRIVDNRAEPMRGVVVRIDSRGRATMTSAMQEQAIEMESAWEVLPVQASAVESG
                     SSWSAPAGLFGRRRHCRAEDDAEQPGTTRVEFVTAYPPGAGEVLGHSCEGKYWFDRAA
                     GCVTRFENIEIDKPANRRTESAGVLRSRSSMPPGWAQRRAAEAEQYELAVRRESSLLR
                     QIQDRPETVEEALRNLDRLWAGMATELTRQDSPFRTLARAKRQRWTAEADQVRQMANY
                     AQRWLGRTAVPWTLPDARGQMVSSETVRKHAGVELLWRTDDPRNIAELAQLQAARDEL
                     GDSEIPVICLNLDQDTSAARRSIAQLPAGLTHILAGPIAAIEQPINLPILRVLDADGK
                     IVRVWIGWQPSCVEAFVEARRCGQ"
     gene            12965..13813
                     /locus_tag="PLU99_05955"
     CDS             12965..13813
                     /locus_tag="PLU99_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOO16638.1"
                     /translation="MFKRAVEWFLLCSIALLGMIGSCARPSSGPVAARIEARPAPVSG
                     LTAAEADTRADRVRQDPAGYLRQVLAKCGELKQYTLEFTRYERRGLLNRLQGPEHIQC
                     WFRRDPFSVRMKWLDEDMKYLESTYVAGQFDNQVRFVTRWAIPLLLPPPAVNKVDLRT
                     PVIWGESKRPMTDFGLERLMEQTVTSMDGAGDAVVVTYQGLFELPETGATVHHIRLVY
                     SDAQHPVPIQELYIDTKNDVPSGTVLKHTDGRIDAAYFYKDLDTNVQLTDQDFLLESE
                     RTLAAQ"
     gene            13910..>14565
                     /gene="ribD"
                     /locus_tag="PLU99_05960"
     CDS             13910..>14565
                     /gene="ribD"
                     /locus_tag="PLU99_05960"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008693889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOO16639.1"
                     /translation="MSRAESEATYMRRALQLAARGQGWVEPNPMVGCVIVRGGQVLGE
                     GYHRRFGGPHAEIEALRKCQRNSKNARGATVFVTLEPCCHQGKTPPCTAALIEAGVAR
                     VVAAMRDPNALVSGGGLRALKKAGIQTEVGLLEREAAELNAPYIKLMRQKRPWVILKW
                     SQSLDGKIATRTGDARWISGESMRAHAHRVRGRLDAIIVGRRTAEHDDPMLTCRIGRP
                     R"
BASE COUNT         2622 a         4378 c         4771 g         2694 t
ORIGIN      
        1 cacccacaaa ggtgcgctgc gtgtcagacg aacttcgtct gcccgggcac cgccaccggt
       61 tcaaccggca aggcgccgaa gtcgtacgtg ggcgggccca gcttctcctc tgagttcagc
      121 gcctgctccc acgtgatcgt ctggccggtg tacgccgcca tgcggcccat gatcgccatc
      181 aacgtgctgt gcgccatcca ctggccatcg ttgatcggcc gcccggagcg aatcgcggcg
      241 aacagcgcgt cgtgttcatt ctggtacata ccgcggcgct ggccgtcgta gctccacttc
      301 agttcacccg cgtggctgcg aatctcgtgc cgcggcttcc atccgtcgat ccggcacact
      361 gcctccgtgc cgacgaagta atccgtattg tcaaacgggc agtcggccat ctgccggcac
      421 gtgtgaaaac aacgcaggcc gtcgtcgtac tcatagatga cgctgaagtg gtcgtaaaca
      481 ttgccggact cggccccgct ccgcgcctgg cggccaccca gggcggtagc tttgaccggc
      541 agccggccgc ccagcgccca ggcaattttg tcgatgctat ggcaagcctg ctcgacgatg
      601 tgatcgccgg acagccaagt gaaatggaac cagttgcgaa gctgccattc catatcagtc
      661 cagccgggtt gccgcggaaa ttgttgcagc gggctggcgt ggtacgcagt gtgcgcagac
      721 acgatgcggc ccagggcgcc gtcgatgatg cgctgaaacg tggcgcgctc cgggtcgctg
      781 tagcgccagc agaagccggc cagcagcgac aaattacgct tgctcgcctc ttcagccgaa
      841 gccaggattg agcggacgcc cggtgcgtcg acggccatcg gcttttcgca gaaaacgtgt
      901 ttgccggcca gaacggccgc tttcaggtgc gctgggcgga agtgcggcgg ggcggccagc
      961 aggaccacgt cgacgccgct gtctatcacc ttctgatagg cgtcgaagcc cacgaatcgg
     1021 cggtccggat cgacctgcac ccgctcgggc tgcgcttcct tcagcgtctg taggctgttt
     1081 tgcaggcgat cgttgaagac gtcgcccatg gcggttagca ccgcgccggg gtcggccgcc
     1141 agcgcctcct ttgcggcgcc agtgccgcgg ccgccgcagc cgatcagtcc gactttcagc
     1201 gtatccgagc cggcggcgta ggcggccggc gcgagcatct cgcgcgtcaa catcgccccg
     1261 gcgactgcgg ccgcggaggc tttgacgaac tgccggcgtg tgacgccggt tgcgtgcgga
     1321 gttgaatcat gccttttcat ggcgcctcct ttggcgagac cgcgttttgg gtcacaacgg
     1381 ctcgcgcttc cccacatgca cacgtgtgaa ggtgggaaag tgggaaagtg ggaaggttga
     1441 acactgttcc gtggacgccg cgatcggcag gctctattcg ccgttacgga gggcgtctgg
     1501 atcagcagct ccggcgcgcg ggatcggcag cacgatccag cgagcgcccc ggattactct
     1561 tcctcttccg ccgcggcttt gtgctgcgcc acaatctgct gctggatgtt gggcggcacg
     1621 atgtcgtagt ggctgaactc catcatgtag ctgccctggc cgccggtggc actttttagc
     1681 tgactgctat aggtggccac ctcggccagc ggcaccgttc cgcgaatgac gatctggttg
     1741 cctggcagca tgtcctggcc cacgatctgt ccgcgcttgc cggcccagtc gcgcgtgatg
     1801 tcgcccacga attcggccgg cacggtgatt tcaatgttca cgatgggctc gagcaggatc
     1861 ggcttgcact gcacgaaggc ctttttgaac acctggcggc cggcgatttg gaaggcgatg
     1921 tccttgctgt cgactgggtg ggttttgccg tctaccagcg agactttgac gtcgaccact
     1981 gggaagccgg cgataacgcc cttgcgcatc tggtcgcgaa cgcccttgtc gacgctgggg
     2041 cggaaggcct gatcgatgac gccgccgaag atcttgtcga tgaactcgta gcctttgccg
     2101 cgctcgttcg gctccatatc gatgaacacg cgggcgaact ggccggcgcc gccggtttgt
     2161 ttcttgtgcg tgtattcgac gtacttgacc gcgcccgaga tagtctcgcg gtacggaatc
     2221 ttcggggctt tggtttcgac ttcgagcttg aagtaacgcc gcattttgct gcggatgacg
     2281 ttcaggtgct ggtcgcccat gccctgcatg accagttcgt tggtttcctg gtcgcggaag
     2341 tactggaagc atgggtcttc ttcggcgaag cgatgcagcg cgccgctgat cttctccacg
     2401 tcagcccgcg acttcggctc gaccgccagc gcgaacatcg gcgtggggaa cttcggcatc
     2461 ggaatctcgc cgtcttcggc cttggcgaag agggtggcgc cgatcttcag atcaagcttg
     2521 gcgaaggcga tgatgtcgcc ggcctcggcg ctttcgattt ccgcatgctg atcgccccgc
     2581 agcttgaaaa agtggccggg ccgctgacct ttgcgctcgc caggcgtgaa aatctgtccg
     2641 tccggcttca gcacgccgcg atggacgcgg gccacggagt acttgatgtt gctgcgcggg
     2701 tccgaagtga tcttgaagac ctgggcgacg aaatcgcccg cgcgatccgg ctcaacctca
     2761 gtcttcttgg ccgcctcacc ctcgcccata accacgctgc ggcgtttgcc ttcggccggc
     2821 gacggcgcgc aacgcagcag ggcctccaaa agttcgggaa ttcccacgcc ggccttggcg
     2881 ttaacgaaca gcaccgggat gagcccgccc gaagccacgg ccttgcggat ggccggcacg
     2941 agctgctcga gcgaagcgct gccggtctcc atgtaggatt ccatcagcgc ctcatcggtt
     3001 tcgacaatgg cgtcgaccag gctggtgtgg gccgcggcca catccagcag atccgacgtg
     3061 ccttgctcct cgcgcagcaa gtcgatcagc ttcttgccgc cgtcagccgg aaggttgatc
     3121 gggtggcagt gatgaccgaa ggtttcgcgg acgccacgca gaatttcgtc gaggttggcg
     3181 ttttcggcat cgatgcgcgt gatgacgatc attcgcggca ggccgtattc gccggccgca
     3241 ttgaacatgc ggcgtgtgtt gacgccgatt ccgctggcgg cggagacgac cacgcaggcc
     3301 aggtcgacgc cggccagggc cgcgatggcc gggccgatgt agtccggcat gccgggcgta
     3361 tcgatgaaat tcagcagctt tccctcgtac tccacgtaga aggcggccga gtcgacgctg
     3421 tgcttacgtt cgcgtgactc gtcgtcgtga tcgagcatgc tggtgccgtc gtcgatgctg
     3481 cccatgcggg tggtcacgcc cgccttgtgc agtatggcct cgccgagggt ggttttgccc
     3541 gcggcggagt ggccgacgag ggcgacgttg cgaatatcgc tgatctggaa agcagccatt
     3601 caccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggcc gggggcggct
     3721 cccataactt ggcgcgcggc gagcgcggtt tggcgtactg caaaccgatc aggccggcca
     3781 ggccgatcag ggaaatcatc agcgctatga actgtgagat cgtgaactgg aaagtgtcga
     3841 ccgggttgtc ggcacggatc gtctccagga tgtaccgcgt ccagggttcg atcagcagca
     3901 tggtacagat cacctgcccg tcgcgcgttc gccgccagta cagtgcgttg agcagcaacg
     3961 ccaagaggat cagtgtgagc gcggaataga gctgcgtcgg atggaccggc aacgccggat
     4021 actgccgggc cagctcctga agctgtgtca ggctcaggcc ggaagcttgc atggccagca
     4081 ggacctccgc atgagctgcg ccgctggcgc agccgcctcc gagcgaagaa gcaagggaac
     4141 caagctcagc ccgcagacgg ttctcctcgc ggtccaactc ggcttgttcg gcggcgctgg
     4201 tggcctgggc gcgccgcgcc gtgagctcct tttggcgctg gccaattgcc gccgcggcgc
     4261 ttgtgatttt ggcaaactcg gcctccgtca ttcgcagcat ttcacgtggc agaggagcgg
     4321 ccgggcggcc atccgtcagc acgccgttgg gcggaaacca caacagttcc tttggaaggt
     4381 cgagttgtcc cgactgccat tgctgaatca acgcgttgct gccgaacgga aagcgcaccg
     4441 cccacggcag gtcagacggc aatccccagc agcagccgtt gaggaagcag ccgagccgtc
     4501 ccagtcccat gcccagcgcg gccgagggcg ccatgatgtc caaataccag cggaacgagt
     4561 gaccccagaa aagcatgtat ataacgacgc ccagcgtggc ggcgatgaac ccgccgtata
     4621 cctccaggcc gccgtcgcgc acgccgaggg tggcccgcaa caggatcgcc acccagtttc
     4681 ccagcggcgc gaactgctcg tcccagtagt gcaccacgta catgagccgc gccccgccca
     4741 cgccgccgat cagggcaatg aaagcgcaat tgagtaccac gtccgggttc ccgccggacc
     4801 tggtagcgcg gcggacggcc cagaccaccg acagcaaaaa gcccagcatc atcaccaggc
     4861 cgtagccggg cacgtcgata ttcacaaccg ggatgcggaa aaccacgggc atcatggttc
     4921 aggtcaacct cgcgaatctg cggccggctt tgatacggcc gatgactgca tatcgccgcc
     4981 ggggctcgaa ccggacatcc taagcgccgc ggcggccggg gtccacgaag aagctgtcga
     5041 tcaagcgggc cgcgccgata tggacggcga gggcgaccag aaccggaccg ctgatgcgtt
     5101 cgaccggctg catggtttcg gaatcgacca ggcggatgta atcgatgcgt gccggcccgg
     5161 cggcggtgat cacgtcgcgc agcagtgcgg tgatcttgga tgcattgcgc tcgccggcgt
     5221 gaatccggtt ccgggctttg atcaagccgt catatagaca agccgcctga tcgcgctcgg
     5281 ccggcgtgag gtacatgttg cggctgctga tggccaagcc gttttcttcg cggacggtgg
     5341 ggcagccgac gatttccaac ggcatatcca agtcgcgcac catgcgccgc acgaccgcga
     5401 gctgctgaaa gtccttttcg ccgaaatagg cgacgtcggg ctgcacgatg ttgaacagct
     5461 tggtgcagat ggtggtgacg ccgtcaaagt gaccggcgcg cgagacgccg cacagcgttt
     5521 cggtgagttt ggcgacgtgc acgcgggtga cggcgtcggg ccggcagagt tcgtcggttg
     5581 aaggaatgaa gaccaggtcg actccggcct gctgacatac ctgcaggtcg gcggcttcgt
     5641 cgcgtgggta cttctggaag tcctcgttag gcccgaactg caacgggttt acgaagatgc
     5701 tgaccacggt gtaggtgccg tcacggcgcg cggcgtcgat cagcgaagcg tgtccacgat
     5761 gtagggcgcc catggtaggt acaaagccga tccgctgccc agagcgtcgc gcctccgcaa
     5821 cggcgcttcg cgtgtcggcg atgttgcgag ttacttgcat cgatctcttt agtcctgcgc
     5881 cagattggcc gggccctctg actgttcctc tcgattccca gtcgccgaac cgccgggtta
     5941 aaatgcggcc acctgttcgg tcaaacacgc acgtatgata ggagtctgac catggcgcac
     6001 cagcggcgcg cgacagtttt cttgagtttg gctgtctgcc tgtggctgcc cggcggcgcg
     6061 gcgcgggctg ccgacccgcc ctggctggcg cgcggccgga ccggcatggt ggccagcgac
     6121 tcgccggagg cctcgcaaat tggggccgat gtgctggccg cgggcgggaa tgcatttgac
     6181 gcagcggtgg ccacgtcgtt cgccctggcg gtggcgcggc cccagagcac cggcctgggc
     6241 ggcggcgggt tcatggtggc ctgggtggct gcggaaaagc gcttcgtggt tttggacttc
     6301 cgcgagtgcg ccccgcaagc cgccacgccc gagcggtacg cagaactggc ggcccagcag
     6361 ggcgacggtt tttcgccgag cgtttacggc ggcaacgcgg tggccgttcc gggacaactg
     6421 gccggactgg tcgagattaa ccggcgcttc ggcacccagc cgctgaagga gctcattcgg
     6481 ccggcggcgg ctctggccga ggcgggcttc gcggtggatg cccattatcg cggcgcttgc
     6541 aaggaagcgc tggcggtgct ggagaagtcg ccggccctga tgctgcgata caaggttctt
     6601 tacgaaacgc tgttgaacaa gggcaaactg ccgcagatcg gcgagcgact caaacgcccg
     6661 gatctggccc ggggcctgcg actgatcgcg caagaggggc cgcgcgtttt ctatgaagga
     6721 ccggtggccg cggcgatcgt aagcgcggtc aaagcgagcg ggggagagct gacgccggag
     6781 gatctgcgga attaccgcgt tctggagcgc gcgccgatcc gcgcggcaat gggcgagtac
     6841 gaactggtca caatgcctcc tccgtcatcc ggaggtgttt gtctgattga ggcgttgaac
     6901 atcctgcagt gcctggccgc cgacgttggc ggggtgacga agctgcgtag caaggattcc
     6961 gacttgtacc cggccgccct ggcgcgggcc ttcaaacacg cctttgccga ccgggcgcgc
     7021 tggttgggcg acccggattt cacgccggtt ccggttgcgc ggttgacgga caaacagtac
     7081 gcgtgcgatt tggcgctgaa agcgggacag aatcctgaag acttcggcag ccggcaactg
     7141 ccggaggatc ggggcacttc gcacttttgc gtggccgacc acctgggcaa cgtcgttgct
     7201 ctgaccgaga ccatcaacgg cgtcttcggc tcgctggtcg tggccgagcc ctacgggatt
     7261 cttctgaata acgagatgga cgattttctg acggtgcgtg ggcaggcgaa tctgtacggc
     7321 ttgctgcagg gcgaggccaa tctcgtgggg cccggcaagc ggccgttatc cagtatgtgc
     7381 ccgaccatcg tcgtgcaggg ggggcgtccg gtgctggcac tgggcggctc cggcggaccg
     7441 cgaatcatca ccggcgtggc ccaggtgctg ctgaacgtga tggagtttgg cgagtcgctg
     7501 gacggcgcag tggaggccgt tcgcctccat caccagtggc agcccgacgt agtgtttttc
     7561 gacgggcagc ctgcggcgga gttgcgggcc gcgctcgagg gggcggggct gaaggtcagc
     7621 gatcggcggc ggggcgcggt ggtgcaggcg ctgcagattc ttccggacgg cacactcgtc
     7681 ggagtcagcg atccgcggcg cagcggtcgg ccggcgggcg tgccgtaaca gaatttgagg
     7741 ttttgcgagg cggggaaaac agtggacctc ccggccattg gcggcgcggg aggtccaaca
     7801 gcgtaccgaa gtcacgccgg aggcaatagt ccgcgtacta ctgcaccaga agcgttccgt
     7861 aggtggggtc gcgggggaag gtatagtcca cccagccggt ccagaggctc tgatagagat
     7921 tcgtcagcgg cgtctggagc agatcggcga ttgtatcgtt agtgttcgcg aggccggccg
     7981 aaacgccctg cgagatggcc tgatttccca cgcccgcgat gaaactcgtg tatgggtcgg
     8041 tagctgcgaa cggccgacaa gtgaatgggg cagccaacac caagccggcc agggccaagc
     8101 aaacaaatct agctttactc atcagttcct ccatgtgttc aaaccaattt ccctaaggct
     8161 aaacgcgttg ggctcggacg gtggccggac caacagcgcc atcgtgtgcg caggaaacga
     8221 aaccgcacca aattgatcta actgtagcaa gatccatacc gtttttggag tcgagaggtg
     8281 gacattcgca acgtcatgcg ccacagggac ttaggcgaat gtggaggagg cgcgcatatg
     8341 cacatcggaa aggcgagaaa tgacggtcgt gcgccaccca ggatgggcga agatgttgga
     8401 actgctgatc ggccaagatg ttatgttcgg tttgccgagg cagtgcgtga cgagcgctga
     8461 cgcgaagccg tcgggcatgt tggccgctgc gtttttcgat ttatcaaatc aagatggttt
     8521 tagtatcgca ttgtccgttg tagcggccgt tgacagcctg cgtccggtcg ggtaactagg
     8581 ggtacgctct gcatcgtagg cggagtgact ggcaggaggt cttgtgtgag cggagctatg
     8641 atgctgctgg ctgagttagg cgcgacgtat tacatcatca tggtggcgct gttgatcggg
     8701 gttctcattc tttggaaagt tctaaaggcc cgcggcatga gctaatctgc cgatgcggaa
     8761 ccttggtgcc gcccccataa gctggtagac gctacgccgc cctttgtgtg tggctcaaaa
     8821 tgccgattcc cgaacgagga ccgcagtcct gctggcctgc gattgcgcgc gatgccatcg
     8881 agcacgcagt acgctatggc gaaaccgcgg ctgattgtgc cgcgccagct cagcatgagc
     8941 ctgagtttgg gggcgtcttc gttaccctgc acaagcttgg ccgcctgcgc ggctgcatgg
     9001 gcctgctgga accgcaagaa tccctaaccg cagccgtgcg ccgggcggcg gtctgtgcgg
     9061 ccttgcagga tccgcgcttt catccggtga cgttggcaga actccccgcg gtggagatcg
     9121 aagtctctgt gatgtcccgg ccccagccgt tgcgcgacct gcagcagctc gaactcggcc
     9181 ggcatgggat catcgtcggc cgcggccgca accgcggttt gttcctgccg caggttgcga
     9241 cggaatacgg attcagccgc gaggaattcc tttcgcgctg ttgcagcgag aaagccggct
     9301 tgccggcgga tgcctggcgc gacccgcgta ccgaagtgtt catcttcacg acggatgttt
     9361 tcgctgaact gagctaggtg cggcattcgc cgccggtccg gtgggatgat ctgatcgccc
     9421 ggcaaatccg ggcggccagg cggttcgccg cgccgagcgt tcatacttcc tgcgcgagtt
     9481 ggctccgcgg atttgttcag tagcggcaat aaagcaacgg gagggcgagg ctcgcgcact
     9541 cgccctcccg gtttgaattc taggggtaga actagcggcg ccggccgagc aacagcagcg
     9601 cgcccgccag cagcgccaag ccagccggtt ccgggatgat ctggaagtag tgcgttccgc
     9661 cgcccagatc tccggcggtc aggctgaagg ccaactgcga gttcatgtcg ctcaccgcaa
     9721 acggaagcgg cgagtagggg acgaacgaca acgtgctggt gctccccgtc ggagtcgtca
     9781 cgcccggcag cacctcctca aaaagggtac cggtggggac cagaccgttg taatgggtaa
     9841 taaggatacc gcccgccgag ggaagggcct gcatggcaac gccgttgttg ttcgtatcgg
     9901 tcacgttgat tgccagcccg gccgcggcgc taggtgtcgc caaggtggcg aaggtcaatt
     9961 gcggcatcgt gattgtgaag atggtgtcgg agctgcccga ttgaaccgcg aaagcaccgg
    10021 cgatcttctt ggtcgagtcc aactgcaacg ccatgttcgc gttctgcagc gtcgcgatag
    10081 gcgtgaagaa ctcgtcgtag atgaactgcg ttgccgtgtt ccaaatgtaa gtggacgtcg
    10141 atgggttgta gaccaactgg tcccgagtga actccaggac acctgtcccg gacgcatttg
    10201 tcgcctcaat cctgaaaatt acttcggaaa tgtcggacaa atctgccagc gcgcccgaag
    10261 ccagcgccaa caccagtcct agcgacaggc atctagttct catgctcgtc tcctctctca
    10321 cctctaccgc tgctccgcgc tgccgtccga acagcacctg gcctacaggc gctgcctagc
    10381 ggcgacgcag cacacaaacc ccgacggcca gcagaagtag tccagccggc tcggggacaa
    10441 tttcgaaatt cgaagaaccg ctgacgaatc cgtacgcaga aagcgagaac gcgatttccg
    10501 cgcccatgtt gctcgcgacg ccgatcggaa cgtgggcccc gccgcctggg taggcgaaat
    10561 tgccttctgc caacgtcgcc ggtggaatca cgtcgatcgg gccgatcgct tccgcgaaag
    10621 tgatcgagtc gttgtagtaa gcgcggtaag ctccgccgcc gggttgcagg ccgaccagcg
    10681 agacaccctc gcccatgata tccataaccg tgaacgcggc gtccgcacgg gcgcttgcat
    10741 ttgcgatcgt cgggaacgac agcaacgccg aagtcaacgt cacgtcagtg gcagcatcgc
    10801 ccgcttgaaa cgaaaacccc atggcgatct gcgggttgga agaaatggcg atgtagctgt
    10861 ccttcacgat ggtgcccacg acctggtcac cactcattag actaatgtct tctgtgttgg
    10921 accagaaaaa gtcgccgccg tcccatatgc cctcatcagc cgtgatttcc aacacgccgg
    10981 taccgcggcc agaggaggca gtgatcctca gcacaacctc ggaaacgtcg gccagcgcgc
    11041 tgccggcgac aaaagacagc acgactacta gtgcgccaag cgcccgcatt cctgatcggt
    11101 ctagtttcat ggcattcctc tttccttgat cggacggacc ttgctgcgcc cgacactcca
    11161 cggcaacatg cagcctcgaa ccgctggcca caaaagccgc ttacgactcc tcagccctac
    11221 aagccaatga catttttacc acacccggca gtatcggttc aagtataaat tgagccggcg
    11281 ggcggcgcga cgcgcttcag cgtacataag tccctgacaa tgaaaggctt acagccgccg
    11341 agaataatcc tgccgcatga aggtggcgat ttccagccag aaagctcatg ttttatagga
    11401 cgatgtggct accagcaccg gcaataatca actgtaaaag ttaaaaaatg cgaaatctct
    11461 tagtactagg acccaaaacc atcccgttgc gggtgcggtg gccgctaggt acaaacgcgg
    11521 gcatgttagc ttgctgtcac tttttggcgc tggcgcaggc gatggttctg ccgagtgtcg
    11581 cgcagggatt cttgtcgcgg ccgccgtcgg cgtaccgctt tgagcccggc gatcggctgg
    11641 tctacgaact tcgctctgag agcacgccgc tcacgcccgg cgcggcgggc gagcgctgtt
    11701 tcggtcagat cgaaatatgg tgcctggctc tcaagaatgg cgaggcgcta ctcctgctag
    11761 atttcatacg cattgtcgat aatcgagctg agccgatgcg cggcgtggtt gtacgtatcg
    11821 acagccgggg gcgggcaacg atgacctcag ccatgcagga gcaggcgatc gagatggaaa
    11881 gtgcatggga ggtcctgccg gtccaagcca gcgcggttga aagcggaagc tcgtggagcg
    11941 caccggccgg cctcttcggc cgccggcggc attgtcgggc cgaagacgac gcagagcagc
    12001 cgggcacgac gcgcgtggag ttcgtaaccg cctatccgcc tggcgctggc gaagtgttgg
    12061 gacattcgtg cgaggggaag tactggttcg accgggctgc gggctgcgtt actcgctttg
    12121 agaacatcga gattgataag ccggccaatc ggcggactga atcggctgga gttctacgct
    12181 cgcgcagcag catgccgccg ggttgggctc agcgccgcgc cgccgaagcc gagcagtacg
    12241 agctggcggt gcgccgtgaa agcagcttgc tgaggcagat acaagatcgt ccggaaacgg
    12301 tcgaggaggc gctgcgcaac ctcgaccggc tctgggccgg catggccacc gagcttactc
    12361 gccaggattc cccattccgc acgctggctc gtgccaagcg gcagcgctgg acggcggaag
    12421 ccgaccaggt ccggcagatg gcaaactacg cccagcgctg gctcggccgc accgccgttc
    12481 cgtggacgtt gcctgatgca cgcggtcaga tggtttctag cgaaactgtc cggaagcacg
    12541 ccggtgtgga actactttgg cgcacggacg acccgcgcaa catcgccgag ctggcacaac
    12601 tgcaagccgc gcgcgacgag ctgggcgatt ctgaaatccc cgtgatttgc ttgaacctgg
    12661 atcaggacac gtccgccgcg cgccgctcga tcgcgcagct tccggcgggc ctgacgcaca
    12721 ttctggccgg gccgatagcg gccatagaac agcccattaa cctgccgatt ttgcgcgtgt
    12781 tggacgccga tggtaagata gtgagggtgt ggatcgggtg gcagccgtcg tgtgtggaag
    12841 cgtttgttga agcgcggcgt tgcggtcaat gacgaacgac tcttgccgcg acagatttgc
    12901 gcgtgccagg cggactgtgg caccctttgt acaccggcgg gccaagctga ccaggagtag
    12961 cggtgtgttc aagcgggccg tggaatggtt cttgttgtgt tcaatcgccc tgctcgggat
    13021 gatcggaagc tgtgcgcgac cgtcgtcggg cccggtggcg gcccgcatcg aagcccgccc
    13081 cgcgcctgtc agcggtttga cggcggccga agcggacacg cgcgccgatc gcgtgcgcca
    13141 ggatccggcc gggtacctgc ggcaggtgct ggccaagtgc ggcgagttga agcaatacac
    13201 cctggagttc acgcgctacg agcgtcgcgg gctgttgaat cgtctgcagg ggccggagca
    13261 cattcaatgc tggttccgtc gcgacccgtt cagcgtgcgc atgaaatggc tcgatgaaga
    13321 catgaagtac ctcgagtcga cgtacgtcgc gggacagttc gacaaccagg tgcgctttgt
    13381 cacgcgctgg gcgattccgc tcctgctgcc tccgccggcg gttaacaagg tggacctgcg
    13441 gacgccggtg atctgggggg agtcgaaacg gcccatgacc gacttcgggc tggaacggct
    13501 catggaacag accgtgacct cgatggacgg tgccggggac gcggtcgtcg tgacgtacca
    13561 aggcttgttc gagttgcctg aaaccggcgc gacggttcac cacatccggc tggtgtactc
    13621 ggatgcgcaa cacccggttc ccattcagga actatacata gacacgaaaa acgatgtgcc
    13681 ttcgggcacg gtcttgaagc acactgacgg ccgtatagac gcggcgtact tctacaagga
    13741 cctggacacg aacgtgcagt tgaccgacca ggattttctg ctggaatcag agcgcacgct
    13801 ggccgcacag taacctggtc cccgcggccg acggcctttc aatacgttgt tctggcgccg
    13861 agccgcttga gaccgcggcg gggtcggcag tgtatattag cgagcactca tgagcagggc
    13921 agaaagtgag gcaacctaca tgcgtcgcgc gctgcagctt gcggcgcgcg gccagggttg
    13981 ggttgagccg aatccgatgg tcggctgcgt catcgtgcgc ggtggccagg tgctcgggga
    14041 agggtatcac cgccggttcg gcggaccgca cgccgagatc gaggcattgc gcaaatgcca
    14101 gcgtaattca aaaaatgccc gcggcgccac ggtcttcgtg acactggagc cgtgctgcca
    14161 tcagggcaag acgccgccct gcaccgccgc tctgatcgag gccggtgtgg cgcgtgtggt
    14221 tgccgcgatg cgcgatccga acgcgctcgt ttcgggcggc ggcctacgcg cgttgaagaa
    14281 agccggcatt cagacggaag tgggtttgct ggagcgtgaa gcggccgaac tgaacgcgcc
    14341 atacatcaag ctcatgcggc agaaacgtcc gtgggtgata ctcaagtggt cccaaagtct
    14401 ggacggcaag attgccaccc gcacggggga tgccaggtgg atttccggcg agagcatgcg
    14461 ggcgcatgca caccgcgttc gcgggcggct ggatgcgatc atcgtcgggc ggcgaacggc
    14521 cgagcacgac gacccgatgc tcacctgccg catcgggcgg ccgcg
//
LOCUS       DAOSYS010000069        14500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3509_length_14500_cov_5.741987, whole genome shotgun sequence.
ACCESSION   DAOSYS010000069 DAOSYS010000000
VERSION     DAOSYS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_14500_cov_5.741987"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLU99_05965"
     CDS             <1..426
                     /locus_tag="PLU99_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO16640.1"
                     /translation="GPEIYMQSLYTWADAAGGTAIHAGYLTDENPAAPPAFPGPEWQN
                     LPVDHWFRQTVLVNMEYFKIIECTIQDLTIGGPVTVAHPDNWHFINYSVAGGTLPTGF
                     RCFTGGTTAGNFLAWDNFSIPEPATIALLLIGFAALRRR"
     gene            580..2121
                     /locus_tag="PLU99_05970"
     CDS             580..2121
                     /locus_tag="PLU99_05970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO16641.1"
                     /translation="MRVLLLRPPRYMWPFNRDESAFWPPLGLLCLAAAVRRELPDVRI
                     EVWDAPGNRWGWRTLAARLAAEPVDVLGIGEETVSAHEGLRAAALVKKLHHGCVVVAG
                     GTYFPYAIEQTLGTGVVDVIVRGEGEVTFVELLRSINDAQNWHAIPGLAFRDQAGQTV
                     VTPARPLIRDLDSLPRPAYDLIDLQRYGCGSRNHPQLVSLEHSRGCVDSCGFCILWKH
                     MGESLNGNGQVRPRVRTKSAERSFEEVHWLYREYGRRTFGWVDPTFNAIPEWSDQWAE
                     RMLRSELVSPRGVARTLHTAWLRADAIVRDEKLGILRKLVRAGLRQAVIGLERDDPRG
                     LTTVGKHHNDAEVCREAIAILREKYPQVYIIGSLIFGLPGDTWADLRRLLRWQDRLRP
                     DYCFIIPLTPNPGTEAARGSLAEVDPDLSHYNFHTPVCATGALDLRSMQGMYWRIMLR
                     PSWPRMQRLLRTVVFERCPRKRRISLSLLVHGTRISVRSLLSRLRAQPAQVNCYSRKP
                     SWYET"
     gene            2106..3443
                     /locus_tag="PLU99_05975"
     CDS             2106..3443
                     /locus_tag="PLU99_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16642.1"
                     /translation="MVRNVVTGRRMAGSAIAAITVGGVALVCHILACNDSPMAWSPDG
                     KQLAFVTLHAYEPQADLLLAGKHLSRLMVVHDAKELRILEETAAEMLCAPAFSPDGTR
                     LSYLRIPLLSADALEEFNQEFESRKQQLSAIAPVEWTAPAQPGAPASAPTSAPALVDA
                     EIGLPNSETLNRYWEKARACPELPAQLVTRNAKDGTVLSATPLSFPGLCEDRLLIAYL
                     TLKPQYDPDGQWVYLTAGNTVLRVNPATSRVERVIAPAAAATLSPDGKVLAVFQDDAL
                     ALIQTDGKKSLYVPAGNAKSMAGFSWTAAGTLVVLEQAEAGSGVVLRFLDAQGALLKT
                     VPIDLPPREEQKNEIERAVLAVAPDGRHAVIELERDVYFVTTAGELLSTWHSEDNLLT
                     QPTFSPDSQRVALKLMQDSERLSAIVFFSPAGQELTRVPIPPIDPAATQPAKP"
     gene            3498..4079
                     /locus_tag="PLU99_05980"
     CDS             3498..4079
                     /locus_tag="PLU99_05980"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOO16643.1"
                     /translation="METLTAVFDFLCGQERCFVVDGAALPVCQRCTGLYVGALLAGLW
                     LFASRARQRGVPPARLAWAYAAVLIAALLGGVHVLDFGPRWRFLCGLWTGQVALVWLF
                     TGATQLWSWACGSWGESLFWSRRDNVGAWAMLALAPVLALASPMPHGTWPVWTAAIVG
                     GALLLGITGLRTAWAAIRAVTASTCSTQPEKCE"
     gene            4231..5904
                     /locus_tag="PLU99_05985"
     CDS             4231..5904
                     /locus_tag="PLU99_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO16644.1"
                     /translation="MSLLLCRATACLAWTCAFSTLALARDAQPAQAPSGPAAPASQAG
                     VDLAARYPTTLEAGDTDPGRARAWTFAQDDIYGLTEFKFGIGDRLQIATGAADVGIGH
                     CADGAVWAVIVPRDAATLSSPQGQQEPITHVWLRFHPREIATLFPPATVHAAGRKELA
                     GLMRRVATVKMNASYQAGGNAMIPPREDMTVDVDVRDGKRRFFVVNTAAKTAEYINAF
                     EARSVPAQKPFDARLAEQAFDKLWKTYDERYAGFVLRPEVDWNALREQYRPQALAAGD
                     PDSFAEVCARMLRPLRDLHIWVSVDGVPVEVYRRLRPVNANPAAWAVLLGQINSGVGR
                     LAWAVTDDHVGYIFIPEWSNPRLPRACDEVLEQMRGTRGLIVDVRLNGGGDETLAQLV
                     AARFADRAITYGYSQYRDGPGHSDLTDKKPRSIEPRGPWRYDRPVVLLIGQRCMSSNE
                     SFVAMMGECPQVTTMGDRTCGSSGNPMMLELGGGITVSLPRWVDLLPDGKPLDERGIE
                     PDIRFEGSPEAFGGQRDDLLSAALAHLRAVPLPAEPISGPALEPEESTP"
     gene            complement(6213..6638)
                     /locus_tag="PLU99_05990"
     CDS             complement(6213..6638)
                     /locus_tag="PLU99_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO16645.1"
                     /translation="MAFEISTVQMWEGDSEDRPGILSDQLAEIMRAGASLEIIVSRPS
                     PRPGGPCKVLLGPLRGEEEAQAARYSGLTRNGEHWLRLEGPDRPWLAAGIARTVADHG
                     INISSFTGAAIGQRCVIYISFATEGDARRAAKVLEPILG"
     gene            6825..7118
                     /locus_tag="PLU99_05995"
     CDS             6825..7118
                     /locus_tag="PLU99_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014011770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOO16646.1"
                     /translation="MQIREFQQLIDRMYSDKDRRRGSAKTFLWFAEEVGELASAIAKH
                     DRENLPAEFADVFAWLVTLANVEGVDLEQAIAKFTGGCPGCGELVCRCDAKLT"
     gene            7293..8489
                     /locus_tag="PLU99_06000"
     CDS             7293..8489
                     /locus_tag="PLU99_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOO16647.1"
                     /translation="MLCLRFSCGLLTGCALLSALLLASDGRGAPPAAPTDLHTYETKY
                     YVLHTDLAPAEARAVVHHITLMAEEYNRRTTGMGRRVDRRLPLYLVQNSGTYFTLGGE
                     PGTAGLYSGDKLLAFVRDPLSTETWHVIQHEGFHQFADAAIGRELPAWANEGLAEYFA
                     EGIFTGDAFYTGLIPPARLRQVKGAVADGKFRPLRDMMQMSLETWNAHIGLAHEKAGH
                     NYDQAWAMVQFLAHGDEGKYQQAFANFLAAVSRKQNWERAWVANFGDDLQAFQKHFDA
                     YWLGLSEDPTARLETEALVSMVTSFYARAFSQRQYFDTFEQFLTAAASEQLKADEDDW
                     LPPEMLVRALPRIITAGEWSLRRSGRQAVLCTAKDATLFEGRFQVESGRVKSVEVLVK
                     PAKKKS"
     gene            9084..9836
                     /locus_tag="PLU99_06005"
     CDS             9084..9836
                     /locus_tag="PLU99_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOO16648.1"
                     /translation="MTITLSRPDALNAMNEASCAELGAALRMAQRDEEVRCIVLTGAG
                     CGFCTGQDLKELRAMRQSGDGVDFAAILRQRYNPLIVRLRTMEKPVLAAVNGPAVGAG
                     FSLALAADLRIAARSASFRMAFVNLGLVPDCAATLTLVQHVGYARAAELCLLGEDLSA
                     EDALRWGLVNRVVPDEELAPAARELAEKLAGQPPRAVALIKRALNRAWTATLDGQLEY
                     EALLQGTAAATPEYAQRLEAFFKRRPASAGEK"
     gene            9932..10876
                     /gene="nadC"
                     /locus_tag="PLU99_06010"
     CDS             9932..10876
                     /gene="nadC"
                     /locus_tag="PLU99_06010"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOO16649.1"
                     /translation="MWSALTLQLVAAACEEDLGTAGDITSRLTAGSDPEVVGRVVVRQ
                     AGVICGLALGPVICRTFSERLNCTLEFRPESPAAGEFADGAQVEAGTSVAVVRGPRSG
                     VLTVERTLLNFLTRMSGVATLTRRFVSAARAVNPNVKILDTRKTLPGWRQLDKYAVRA
                     GGGENHRFGLYDAILIKDNHLADVPIDRLRDTLTRWLERVPRTAITECKKGGAAGKAA
                     LSPDFVEVEVDNLEQFAEVCKVGRVDVVLLDNFSREQMRAAVKLRDDLGLKGRLELEA
                     SGGVRLEDVGEIAATGVDRISVGALTHSAVALDIALEI"
     gene            10913..13225
                     /locus_tag="PLU99_06015"
     CDS             10913..13225
                     /locus_tag="PLU99_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16650.1"
                     /translation="MKRQKTSRAAGVAKSDAERRARSMTTAKGGATGREPQASWIDRH
                     FTQLLIGLCLVGLIIRCIILAGYCGTPLALQPVNDARVYWEWAGDIAAGKVVLDHPFF
                     SAPLYPYLLGGIRWLGAQTTLVYVLQMLGDLAAVVLLASVCRGRFGAGVALLAAGLYL
                     ALLEPASAMLRILASSLHLMLVVLVWAALVRVQNRQSWQRLLAVGAALGLLAVAYAPA
                     VLLLPAVAVWLFAICGSRRAPADGVPEESKGCPGAAKAGLATSDAAHTGLMLRVGKAL
                     LPAVVAAAIIAPATIHNWYASGGGFFPVQAGGGITLLQGNYANSTGLYTPIPGVSAAR
                     ELMHDDTQTVYREATGREPHWADVDCYFRDQALHLWRSQPAKLVELAARKLYLFISSR
                     HYADIYPPTDEMAQGVNTRLRLTPLPLPWLTGPALVGLVLMLRRPRLCAPEWMLLALP
                     LLVVVTFFYSPRYRVPAIPIVVIAAAWAFVYALRRRARWPVLGTVVLGLGLGILLEPV
                     NKHFNVDRPSRTATLYNLAYALRLEHRLDEAIDALRKGLEISPQDGPERVMLAEALAE
                     AGRHEEAVVEFRRALSANSAAGELAIKLARSLLALERLADADQVLTAAVEREPHNVML
                     LAMLASTKYRAGQSGAACAYYDKALYLASNEVPLLIEYGNVLISLGRWDEAHTQFEHA
                     LYVEPDNFLANYRMAVIAGHAGRYEEARQRFERVASIQPNNHEIVYDMGYLEMIQGHR
                     EEAIRLLRKALELNPNYEPAKDRLRELGQG"
     gene            13343..14488
                     /locus_tag="PLU99_06020"
     CDS             13343..14488
                     /locus_tag="PLU99_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO16651.1"
                     /translation="MPLVDLRPQATRRSPEEMLAHLASFELELKFSAGIWFFSPAGSR
                     FHARYGPELTIPQRLEIAAGLKNYGLAALEAHYPNEINEQNLDLWRQFTRDTGIRLLT
                     IVPLLFWDEQFEWGSLSNPLDAPRRAAIERTKRALALNKEFDTDFAVVWPGIDGYENP
                     FGQDFIAMRDRFAAGLAEAMDAIPGVRVAFEPKPYEPRGHILFGTTPEGLLLGHHVEG
                     LLRNAENRRLLEAGHALCCLNPEIGHVLMGFEDLAYAFSWPLSEGRLAHSHWNSQPLG
                     NYDQDLNVGAVSPEQLEALLYTLKMYGYRGYFGIDINPERMPVDVALKINFDAIRAAN
                     DRINGLDHEAILHAVNHPDRARGWLEAYLIRARAAHPERLPALPRVC"
BASE COUNT         2552 a         4456 c         4858 g         2634 t
ORIGIN      
        1 gggccggaga tctacatgca gagcctgtac acctgggccg atgccgcggg aggcaccgcc
       61 attcacgccg gctatctgac cgacgagaat ccggccgctc cgccggcttt ccccgggccg
      121 gaatggcaga acctccccgt agaccactgg ttccggcaaa ccgtcctggt gaatatggaa
      181 tacttcaaga tcatagagtg caccatccag gacctgacca tcggcggccc agtcacggtc
      241 gcccaccccg acaactggca ctttatcaac tattcggtgg ccggcggaac gttgcccacg
      301 gggttccgct gttttaccgg cggcacgacg gccggaaatt tcctggcgtg ggacaatttc
      361 tcgattcctg agccggcgac catcgctctt ctgctgatcg gcttcgccgc gctgcgtcgg
      421 cgctaactcg atcctcgcga ggatccttgg gagcttcagc cacccggtcc aatagaccgg
      481 gtggtctttt attaccgcct gtttgccagc actatgtcac tgtccttgca gaggcgctgc
      541 aaacggggca aacttgaagc ctttcaggcg gagatagtca tgcgggtcct gctcctgcgc
      601 ccgccgcgat acatgtggcc ttttaaccgc gacgagtcgg ccttctggcc gccgctcggt
      661 ctgttgtgcc tggccgcggc cgtgcgcaga gagttgccgg atgttcgcat tgaagtctgg
      721 gacgcgccgg gcaaccgctg gggctggcgg acgctggctg cgcggctggc ggccgagccg
      781 gtcgacgtcc tcggcatcgg cgaggaaacc gtctccgcac acgaggggct acgggcggcc
      841 gcactggtaa aaaagttgca tcacggctgc gtggtggtgg cgggggggac gtacttccca
      901 tacgcgatcg agcagacgct gggcaccggc gtcgtggacg tgatcgtccg cggcgaaggc
      961 gaagtcacct tcgtggaatt gctgcgcagc atcaacgacg cgcagaactg gcacgccatc
     1021 cccggcctgg cttttcgcga ccaggccggg caaacggtcg tcacgcccgc ccgcccgctg
     1081 atacgcgacc tggattccct gccgcggccg gcctatgacc tgatcgatct gcaacgttac
     1141 ggctgcggtt cccgcaatca cccgcagctc gtcagcctcg agcattcgcg cgggtgcgtc
     1201 gactcttgcg ggttctgcat cctctggaag cacatgggcg agagcctcaa tggcaacggc
     1261 caggtgcgcc cgcgcgtgcg aaccaagagc gcggagcgca gcttcgagga agtgcactgg
     1321 ctgtaccgcg agtatggccg ccggaccttc ggttgggtgg acccgacctt caacgccatc
     1381 cccgaatggt cggaccagtg ggccgagcgc atgctgcgct cggaactggt ttcgccgcgt
     1441 ggcgtcgcgc ggaccctgca tacggcctgg ctgcgggcgg atgccatcgt gcgcgatgag
     1501 aagctgggaa tcttgagaaa gctggtgcgg gccggcctgc gccaggcggt tataggcctg
     1561 gagcgcgacg acccgcgcgg cctgaccacc gtgggcaaac accacaacga cgcggaagtc
     1621 tgccgcgagg ccatcgccat cctgcgcgag aaatacccgc aggtgtacat catcggcagc
     1681 ctcattttcg gcctgccggg cgatacctgg gccgacctgc ggcggctgtt gcgctggcag
     1741 gaccgcctgc gccccgacta ctgcttcatc atcccgctga cgcccaatcc ggggacggag
     1801 gcggcccgcg ggtctctggc cgaggtggac ccggacctga gccactacaa cttccacacc
     1861 ccggtctgcg ccaccggagc actcgacttg cgttcgatgc agggaatgta ttggcgtata
     1921 atgctccggc cgagctggcc gcgaatgcaa cgtttgctgc ggacagtggt cttcgaacgc
     1981 tgcccgcgaa agcggcgcat cagcctgtcc ctgctggttc acggcaccag aatctcggta
     2041 cgctctctgt taagtagact ccgggcgcag cccgcgcaag tgaactgcta ttcgaggaag
     2101 ccatcatggt acgaaacgta gttacaggcc ggcgcatggc gggttcggct atagcggcga
     2161 tcacggtggg cggcgtggcc ctggtgtgcc acatcctggc atgcaacgac agcccgatgg
     2221 cctggtcgcc ggacggcaag caactggctt ttgtcaccct gcacgcgtac gaaccgcagg
     2281 ccgatctgct gctggccggc aagcatctgt cacggctgat ggtcgtccac gacgcgaaag
     2341 agctgcgcat tctggaggaa acggcggccg agatgctctg cgcgcccgcc ttcagtccgg
     2401 acggcacgcg cctctcatat ctgcgaatac cgttgctgag cgcggacgcc ctcgaagagt
     2461 tcaaccagga atttgaaagc cgcaagcaac agcttagcgc catcgcgccg gtggagtgga
     2521 ctgcacccgc acagcccggc gctccggcca gtgcccccac ttcggctccg gcgctggtgg
     2581 atgcggagat aggcttgccc aatagtgaga ccttgaacag gtattgggaa aaagcacgcg
     2641 cctgcccgga actgccagcg caactggtga cccgaaacgc aaaagatggc acagtcctct
     2701 ccgcgacccc gctcagcttt cccgggctgt gcgaggaccg ccttttgatc gcctacctga
     2761 cgctcaagcc gcaatacgac ccggacggcc agtgggtcta tctgacggcc ggcaatacgg
     2821 tgctgcgcgt caaccccgcc acttcccggg tggaacgcgt gatcgcgcca gccgccgcgg
     2881 cgaccctttc gcccgacggt aaagtgctgg ccgtcttcca ggatgacgcg ctcgccctta
     2941 tccagacaga tggcaagaag tcgctctacg tgcctgctgg aaatgcgaag tccatggccg
     3001 gattctcctg gaccgccgct ggtacgctag tggtgctgga gcaggccgag gccggctcag
     3061 gtgtcgtcct gcgattcctg gacgcccagg gcgccctgct caaaaccgtg ccgattgacc
     3121 tgcccccgcg cgaggagcag aaaaatgaaa tcgaaagagc cgttctggcc gtcgctccgg
     3181 atggacgcca cgccgttatc gagctggaac gcgacgtcta cttcgtaacc accgccggcg
     3241 aactacttag tacctggcac agcgaagaca atttgctgac gcagccgacc ttctcgccag
     3301 acagccagcg cgtcgctctc aaactcatgc aagactccga aaggctctcg gccatcgtct
     3361 tcttctcgcc cgcgggccag gagctgacgc gcgtgccgat tccgccgatt gacccggcgg
     3421 cgacgcagcc cgcgaaaccc tagtggacgt cgcacagtgg attgcattcg tcgcaccatc
     3481 gtctgtcact gccgcatatg gaaacgctga cggcggtctt tgactttctg tgcgggcagg
     3541 agcgctgctt cgttgttgac ggcgcggcgc tgcctgtctg tcagcgctgt accggcctgt
     3601 acgtcggcgc gctcctggcg ggtctttggc ttttcgcgag ccgcgcgcgg caacgtggcg
     3661 ttccgccggc tcggctcgcg tgggcctatg ccgcggtcct gatcgcggcg ctgctcggcg
     3721 gcgtccacgt cctggacttc ggaccgcgct ggcgcttcct gtgtggcctg tggacgggac
     3781 aggtagcgct cgtttggcta ttcaccggcg cgacgcagct ttggtcctgg gcgtgcggca
     3841 gttggggcga gtcactgttt tggtcgcggc gagacaatgt gggagcatgg gcgatgctgg
     3901 cgttggcgcc cgtcctcgcg ctggcgtcac caatgccgca cgggacgtgg cccgtgtgga
     3961 cggccgcgat agtgggcggc gcattgctgc tgggaatcac agggttgcgg acagcatggg
     4021 ccgccattcg ggccgtaacc gcgtccacgt gttcgacgca acctgaaaag tgcgagtagg
     4081 atccggctcc gccgcactgg agagcatgcg gcgtgtgctg catgcaatct aggtgagcgg
     4141 cgtaatcacg taatcatttg agggctgcgg cgcgacattt cgtatgaaaa tatgaacacg
     4201 cgctccccgt cgcccacgaa aggagaagaa atgtcgctgc tgctctgccg cgcaactgcg
     4261 tgcctggcct ggacttgcgc cttttcgacg cttgctctgg cgcgtgacgc ccaacctgcg
     4321 caagccccat ccggaccggc cgcgccagcc agccaggccg gcgtcgacct cgccgctcgc
     4381 tacccgacaa ctctggaggc gggcgacacc gatccggggc gggcgcgggc ctggactttt
     4441 gcacaagacg acatctatgg gctgactgag ttcaagttcg gcatcggcga ccggttacag
     4501 atcgcgaccg gagcggcgga cgtcgggatc ggccactgtg ccgacggcgc ggtgtgggcc
     4561 gtgattgtgc cgcgcgacgc cgccacgctg agcagtcccc agggacagca ggagccgatc
     4621 acgcacgttt ggctgcgctt ccacccccgg gagatcgcca ccttgtttcc gcccgcgacg
     4681 gtgcatgcgg ccggccggaa ggagttggcg ggcctgatgc gccgggtggc gaccgtcaag
     4741 atgaacgcca gctaccaggc cggtggcaac gccatgatcc cgccgcgcga ggacatgacg
     4801 gttgacgttg acgtgcgcga cggcaaacgc cggttcttcg tggtgaatac tgcggcaaaa
     4861 acggccgagt acatcaatgc ctttgaggcc cgcagcgtgc cggcgcagaa gccattcgac
     4921 gcccgcctgg ccgagcaggc cttcgacaag ttgtggaaga cttacgatga acgctatgcc
     4981 ggtttcgtgc tgcggccgga agtggactgg aatgccttgc gcgagcagta cagaccccag
     5041 gccctggctg ccggcgatcc ggatagcttc gctgaagtgt gcgcccgcat gctgcggccg
     5101 ctgcgcgacc tgcatatctg ggtcagcgtc gatggggtac cggtcgaagt ctaccgtcgt
     5161 ctgcgtcccg tcaacgcgaa tccggccgcc tgggcagttt tgctggggca gatcaattca
     5221 ggggtggggc gactggcttg ggcggtgacg gacgaccatg ttggctacat attcattccc
     5281 gaatggagca atccacgtct gccgcgggcc tgcgatgaag tccttgagca gatgcgcggc
     5341 acgcgcggcc tgattgtaga cgtgcgcctg aacggcggcg gcgacgaaac gctggcacaa
     5401 ctcgtggccg cgcgttttgc ggatcgcgcc attacctacg gttacagcca gtaccgcgac
     5461 gggcccggcc actccgatct cacggacaag aaaccgcgca gcatcgagcc gcgcggtcct
     5521 tggcgctacg accggccggt ggttctgctg atcgggcagc gctgcatgag cagcaatgag
     5581 tcgttcgtgg ccatgatggg cgaatgcccg caggtcacga ccatgggcga ccgtacctgc
     5641 ggctcaagcg gtaatccaat gatgctggaa ctgggcggtg gcatcacggt cagtctgccg
     5701 cgatgggtcg acctgctgcc cgacggcaag ccgctcgacg aacgcggcat cgaaccggac
     5761 attcgcttcg aaggctcgcc cgaggcgttc ggtgggcagc gcgatgatct gctgtcggcc
     5821 gcgctggcgc acctgcgtgc tgtgccgctg ccggctgaac cgatcagcgg accggcactg
     5881 gagccggagg aaagcacgcc gtagccagcc ggcgccgccg ggcgatacgc ggcctgcaac
     5941 caacacggcg gccgatcaat caacgtggca gagggacgaa tccagctagc cggctttgga
     6001 cgccgcccag cgtgtttgcc gtcttcgtct ttgccactgt gaggccgcgg atgccggcag
     6061 actcacaaat aggcgattac gagataggcc tcgccgcacc ttattctctg cggtcttggc
     6121 gctttcggcg tcttggcggt tgccttggtt gcgcagagga gaaacttctg gcggcatttc
     6181 ggggcgacac gccccggctc tgctctcggg ggttacccga gtatcggctc caagaccttt
     6241 gccgcgcggc gcgcatcgcc ttcggtcgcg aacgaaatgt agatcacgca gcgctgcccg
     6301 atggccgcgc cggtgaagga actgatgtta atgccgtggt cggcgaccgt gcgcgcaatg
     6361 cccgccgcca gccagggccg gtccgggcct tccaggcgca gccagtgttc accgttgcgg
     6421 gtcagacccg agtaccgcgc ggcctgagcc tcctcctcgc cgcgcagcgg acccagaagc
     6481 actttgcacg ggccgcccgg ccgggggctg gggcgcgaga cgatgatctc caaagacgcc
     6541 ccggcccgca tgatctccgc aagctggtcc gacaggatgc ccgggcggtc ctcgctgtcg
     6601 ccttcccaca tctggacggt cgatatttcg aatgccatag ctcacccacc ggccgccgcg
     6661 acccgccccg taccggtagc gggcggtcgc gcagccctaa gcacgagtcg tgcgtcccac
     6721 ggcacgagca gcctcaagcc tgcaagcgcg gtatactctg cgccgcaagt ataccggtgt
     6781 cggccccaaa tcggccgtaa gaaatcgtgg aaaggcaggt gttcttgcaa atccgtgagt
     6841 tccagcaact gatcgaccga atgtattccg ataaggaccg ccgccgcgga tcggccaaga
     6901 cctttttgtg gttcgccgag gaggtcggcg agctggccag cgccatcgcc aagcatgacc
     6961 gcgaaaacct gccggccgag ttcgccgacg tcttcgcctg gctggtcacg ctggccaatg
     7021 tggaaggcgt ggatctggag caagccatcg ccaagttcac cggcggctgt cccggctgcg
     7081 gcgagttggt ctgccgttgc gacgcgaagc tgacgtagct cggtaaccgt tggcgccaga
     7141 cgcaagctga aagagggatc aagggatcta gggatcgagg gatcaagaag gcgcgtcggc
     7201 ccgcccgaag agctccggag gtcagcgaga gttctttgac cggcgcgcgt gaattgacgc
     7261 gcggcggact gaacgaaagg gaggaagctc atatgttgtg tttgcgtttt agttgtgggc
     7321 tgcttaccgg atgcgcgctg ctgtccgcat tgctgctggc gagcgatggc cgcggtgcgc
     7381 cgcccgccgc gccgacggat ctgcacacct atgagacaaa gtactatgtt ctgcacaccg
     7441 acttagctcc ggcggaagcc cgcgccgtcg tgcaccacat cacgctgatg gccgaagagt
     7501 acaaccggcg cacgaccggc atgggaaggc gcgttgatcg gcggctgccg ctctacctcg
     7561 tgcagaattc agggacctat ttcacgctgg gcggcgaacc gggcacggcg ggtttgtact
     7621 cgggcgacaa gctgctggcc ttcgtccgcg acccgctgtc gaccgagacc tggcacgtca
     7681 tccagcacga gggcttccac cagttcgccg acgcggccat cggtcgcgag ctgccggcct
     7741 gggccaacga gggcctggcg gagtatttcg ccgagggcat tttcaccggt gacgcgttct
     7801 acacggggct aattccgccc gcgcgcctgc gccaggtgaa aggggctgtg gccgacggca
     7861 agttccgccc gctgcgcgac atgatgcaga tgagccttga aacctggaac gcccacatcg
     7921 gcctggcgca cgaaaaggcc gggcacaact acgatcaggc ctgggccatg gtgcagttcc
     7981 tggcgcacgg cgacgagggc aaataccagc aggcgttcgc gaacttcttg gccgcggttt
     8041 cacgcaagca gaactgggag cgggcctggg tagccaactt cggcgacgac ttacaggcgt
     8101 ttcagaagca ctttgacgcg tactggctgg ggctgtcgga agacccgacg gcgcggctcg
     8161 aaacggaggc gctcgtttca atggtcacga gcttctatgc gcgggctttc agtcagcgac
     8221 agtacttcga cactttcgag cagtttttga cggccgccgc gagcgagcag ctcaaggccg
     8281 acgaagatga ttggttgccg cctgaaatgc tggtgcgcgc gctgccgcgc atcatcaccg
     8341 cgggcgagtg gtcgctccgg cgcagcgggc gacaggcggt gctctgcacg gccaaggacg
     8401 ccaccctctt cgagggccgg ttccaggttg aaagcgggcg tgtgaaaagt gtggaggtgc
     8461 ttgtcaagcc tgccaagaaa aaatcgtgag caatgtcgaa cttggcaact tgcgttccgc
     8521 ggcggcgcgg acgcgaggcc gaaaaaacat gtgcattttt gctaggtttt agcttggcgg
     8581 aaggctatac ttgaggcggg aacagggacc aaaatggacc ggcccctgcg ttgtgcgaca
     8641 ggaagaattc gtttgcgcga accgttaagc tgaacctagg gagaccgcag cagtctccgt
     8701 cccgacggca gcgcctgcga gcgctaggtc aggatcgacg gcggccaccc accttattta
     8761 agggttcagc aaacctcttc caccggcaac gtggaggttg ttccgtatat gagtgacggg
     8821 tgagcgccgc aaggcgccgt cgcccgtcgc tctttttatg aaatagagtg cccttgccgg
     8881 ccccgtctca gggccgccga gcgggcagcg cgctgatgcg agatgcggca atccggacgc
     8941 cccggctctg ctctagcggt tgttcaacgg acaccgcgtt cgcccgacgc acgcggtcgg
     9001 gcttctgtca ccgggcgagg cccggtaaga tgccgggcat ggcggcgttt tctaccgtac
     9061 aagcggcggc ggagcggggg gtgatgacga tcactctgag ccggccggat gcgcttaacg
     9121 cgatgaatga ggcgtcgtgc gcagagctcg gggcggcgtt gcggatggcg cagcgggatg
     9181 aagaggtacg ctgcatcgtg ctgaccgggg ccgggtgcgg gttctgcacc gggcaggatc
     9241 tcaaagagct gcgggccatg cggcagagcg gcgatggggt tgattttgcc gctattctgc
     9301 ggcagcggta caacccgctg atcgtgcggc tacggaccat ggagaagccg gtgctggcgg
     9361 cggtgaatgg gccggccgtg ggggcggggt tcagcctggc gttagcggcg gatttgcgga
     9421 tcgcggcgcg cagcgcctcg ttccggatgg cgtttgtgaa ccttggcctc gtgccggatt
     9481 gtgcggcgac gctgacgctg gtgcagcatg tggggtacgc gcgggcggcc gagctgtgtc
     9541 tgctgggaga agacctgagc gcggaggacg cgctgcgctg ggggctggtc aatcgcgttg
     9601 tgccggatga ggagctggcg ccggcggcgc gtgagctggc ggagaagctg gccgggcagc
     9661 cgccgcgagc ggtggcgctg ataaaacgtg ccctcaatcg ggcgtggaca gccaccttgg
     9721 atgggcagct cgagtacgaa gccctgctgc aaggcacggc cgccgcgacg cctgaatacg
     9781 cgcagcggct ggaagcgttt ttcaagcggc gtcctgcgtc ggcgggtgaa aaatagttct
     9841 catggaagtg ggctatcaga cagcatctcc ggtgcgagcg attcggcaat gcgcctcggc
     9901 tctgttgggt tggcggcgcg cagggtgatt tatgtggtca gcgcttacgc ttcagcttgt
     9961 tgcggcggcg tgtgaagagg atttgggaac ggccggcgat atcacttcgc ggctgacggc
    10021 ggggtcggat ccggaagtgg tgggacgtgt ggtggtgcgg caggcgggcg tgatttgcgg
    10081 cctggcgctg gggccggtga tctgccggac tttttccgag cggttgaatt gcacgctgga
    10141 atttcgcccg gaatcacctg ccgcaggcga attcgccgac ggggcgcagg tcgaggccgg
    10201 cacgagtgtg gcggtagtgc gcggaccgcg ctcgggcgta ctgacggtgg agcggacgct
    10261 gctcaatttt cttacgcgga tgagcggcgt ggcgacgctc acgcgccggt tcgtcagcgc
    10321 ggcgcgggcg gtcaatccga acgtgaaaat cctcgacacg cgcaagacgc tgccgggctg
    10381 gcggcaattg gacaaatacg ccgttcgggc cggcgggggc gagaatcacc gtttcggctt
    10441 gtacgacgca attttgatca aggacaacca cttggccgac gttccaatcg atcgactgcg
    10501 ggatacgctg acgcgctggc tggagcgcgt gccgcggacc gcgatcaccg aatgcaaaaa
    10561 gggcggtgcg gccggcaagg cggcgctttc gccggacttt gtggaagttg aagttgataa
    10621 tctggagcaa ttcgccgagg tgtgcaaagt ggggcgggtc gacgtcgtgt tgctcgacaa
    10681 tttttcgcgc gagcagatgc gggcggccgt gaagctgcgc gatgatttgg ggcttaaagg
    10741 tcggctcgaa ttggaagcca gcggcggcgt gcgactggaa gacgtgggcg agatcgcggc
    10801 caccggcgtg gatcgcatct cggtgggagc tttgacgcat tcggcggtcg ctctggacat
    10861 cgcgcttgaa atatagcgtc ggttgcgagg gcggacgtca gcggaaggtt ggatgaaacg
    10921 acagaagact tcgcgtgcgg ccggagtggc gaaaagcgac gccgaacgcc gcgctcgctc
    10981 aatgaccacg gctaaaggcg gggcaacagg tcgcgaaccg caagcgtcct ggattgaccg
    11041 gcatttcacg caactcctga tcgggctttg cctggtaggg ctgataattc ggtgcattat
    11101 tctggccgga tactgcggga cgccgctggc cctccagccg gttaacgacg cccgagtcta
    11161 ctgggagtgg gcgggcgaca ttgcggccgg caaagtggtt ctggatcatc cgttcttttc
    11221 cgcgccgcta tacccatatc tactgggcgg tatacggtgg ctgggggcac aaacgacatt
    11281 agtctatgta ctgcaaatgc tgggcgacct ggcggcagtg gttttactgg cgtcagtctg
    11341 ccgcgggcgc ttcggggcgg gcgtggcgct gctggctgcg gggttgtact tggcgttgct
    11401 cgaaccggcg tcggccatgt tgcggatact ggccagctca ctacatctaa tgctggttgt
    11461 tctggtgtgg gccgcactgg tgcgcgtgca gaaccgccag tcatggcagc ggctgctggc
    11521 agtgggcgcc gcactcggcc tattggccgt ggcgtacgcg ccggcggtcc tgttgctgcc
    11581 ggcggttgcg gtatggttgt tcgcgatatg cggttcccgc cgtgcgccgg ccgatggtgt
    11641 gccggaagaa tcaaaagggt gcccgggtgc ggcaaaagcc ggccttgcga cgtcggacgc
    11701 cgcgcacacc gggctgatgc tacgggtggg taaagccctg ctgccggcgg tggtggcggc
    11761 cgcgattatc gcgccggcca cgatacacaa ctggtatgcc agcgggggcg gctttttccc
    11821 ggtccaggcc ggcggcggca tcacactctt gcagggcaac tacgctaatt ctaccggttt
    11881 atatactcca atccccggcg tcagcgccgc gcgcgagctg atgcacgacg atacacaaac
    11941 ggtataccgt gaagctacag gtcgcgagcc gcattgggcg gatgtagatt gctatttccg
    12001 cgaccaggcc ttgcatctat ggcgctcgca gcctgccaaa ctggtcgagt tggcggcacg
    12061 caagctctac ctgttcatat cctcgcggca ttacgcggac atttatccgc cgacggacga
    12121 aatggcgcag ggcgtgaaca cacggctccg cctcacaccg ctgccgctgc cgtggctaac
    12181 aggtccggcg ctggtgggct tggtgctgat gctgcgcagg ccccggcttt gtgctcccga
    12241 gtggatgcta ctcgcgttgc cgctactggt agttgtaacg tttttttact cgccgcgcta
    12301 ccgtgtgccc gctattccga tagttgtcat tgcggcagcg tgggccttcg tatatgcgct
    12361 acggaggcgc gcacgttggc cggtgctcgg gacggttgtg ctggggctcg gcttgggaat
    12421 attgctcgaa ccggtcaata agcacttcaa cgttgaccgg cccagccgca ccgccaccct
    12481 ctacaacctg gcctatgccc tgcgcctgga acaccggctg gacgaggcga ttgacgcgtt
    12541 gcgcaaaggg ctggaaatca gcccgcagga tggccccgag cgtgtcatgc tggcagaggc
    12601 gctggccgaa gccggacgcc atgaagaggc cgtggtcgag ttccggcgcg ccctgagcgc
    12661 gaactccgcg gcgggagagc tggcgattaa actcgcgcga agcctgcttg ctctggagcg
    12721 gttagccgat gccgaccaag tgttaacagc ggccgtggag cgcgaaccgc acaatgtcat
    12781 gcttctggcg atgctggcca gcacgaagta tcgcgccggc cagagcggtg ccgcctgcgc
    12841 gtattacgac aaagcgctat acctggcgtc aaacgaagtg ccccttctca tagagtacgg
    12901 caacgtactg atttccctgg ggcgctggga cgaggcccac acgcagtttg aacacgctct
    12961 ctatgttgaa ccggacaact ttctggccaa ctatcgcatg gcggtgatcg ccgggcacgc
    13021 cggacgctat gaggaagcac ggcaacgctt cgaacgcgtt gcgagtattc agccgaataa
    13081 ccacgaaatc gtctacgaca tgggctacct cgaaatgata caaggtcacc gggaagaggc
    13141 gatccggctg ctgcgcaaag cactcgaatt gaatcctaac tacgagccag caaaggacag
    13201 gctgcgagaa ctcgggcagg gttaacgccg gctaaggcgc ggcagggatc gccgcgtgcc
    13261 ggaccatcgc cggccgccta tactggccgg cgcaacgcag tagccataga acgttgcgaa
    13321 gccatcgaga aaggagccga ccatgccgct cgttgacctc cgcccgcaag ccactcgccg
    13381 cagccccgaa gagatgctgg cccacctggc gagcttcgaa ttggaactca aattctcggc
    13441 cggcatctgg ttcttctcgc cagccggcag ccgcttccac gcccgctacg gccctgaatt
    13501 gacgattccg cagcggctgg agatcgcggc cgggctcaaa aactacgggc tggcggctct
    13561 cgaggctcac tatccgaacg agatcaacga acagaatctc gatctatggc ggcagttcac
    13621 gcgcgacacc ggtattcgcc tgcttacgat tgtgccgctg ctattctggg atgagcagtt
    13681 cgaatggggc tcgctctcaa acccgctcga cgctccgcgg cgcgcggcga tcgaacgcac
    13741 caagcgcgcc ttagccctca acaaagaatt cgacaccgat tttgcagtcg tctggcccgg
    13801 catcgacggc tacgaaaatc cgttcgggca ggatttcatc gccatgcgcg accgcttcgc
    13861 cgccggcctg gctgaagcca tggacgcaat ccccggtgtc cgtgtcgcat tcgagcccaa
    13921 gccatatgag ccgcgcggcc acattctctt cggcaccacg cccgagggcc tgctactagg
    13981 gcatcacgtc gaaggccttc tgcggaacgc ggagaaccgg cggctgctgg aagcggggca
    14041 tgcgctgtgc tgcctgaacc ccgagatcgg acacgtgttg atggggtttg aggatttggc
    14101 gtacgccttc agttggccgc tctcggaggg tcgtttggcg cacagccatt ggaacagtca
    14161 gccgctgggc aactacgacc aggatttgaa cgtgggggca gtatcgccgg aacagctcga
    14221 ggccttgctc tatacattaa agatgtacgg ctaccgcggg tattttggca ttgacatcaa
    14281 ccctgagcgc atgccggtgg atgtcgcgct gaaaatcaac ttcgacgcga ttcgggcggc
    14341 aaacgaccgc atcaacggcc tggatcacga agcaatactt cacgccgtca atcatccgga
    14401 ccgggcgcgc ggctggttgg aagcgtatct cattcgcgcg cgggcggcgc atccggagcg
    14461 actcccggct ttgccgcgcg tatgctgatg cttacgacct
//
LOCUS       DAOSYS010000070        14243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3588_length_14243_cov_5.507824, whole genome shotgun sequence.
ACCESSION   DAOSYS010000070 DAOSYS010000000
VERSION     DAOSYS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_14243_cov_5.507824"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1779
                     /gene="pgm"
                     /locus_tag="PLU99_06025"
     CDS             130..1779
                     /gene="pgm"
                     /locus_tag="PLU99_06025"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOO16652.1"
                     /translation="MAISTLAGKPAPKQLLVDLAALEREYYERKPDLGDPNQLVIFGT
                     SGHRGSPLRGTFTEAHICAITQAICDYRRSQGIDGPLYMGKDTHALSGPAQRTALEVL
                     AANGVETIIQRDDGVTPTPVISRAILAHNRGRQEHLADGIVVTPSHNPPEDGGFKYNP
                     PDGGPADTSVTRCIEERANQLLRPNNAGVKRLPLAAAMKADTTHETDLITPYVQDLRN
                     VIDMDAISAAGLSLAVDPLGGAALPYWAPINAVYHLDITVVNPKLDPTFSFMTVDHDG
                     KIRMDCSSPYAMARLVNLKDQYRVAFANDPDADRHGIVTPSAALMNPNHYLAVAIQYL
                     LTHRPYWPQQAAIGKTLVSSSMIDRVVEKLGRRLFETPVGFKWFSPGLFDASLCFGGE
                     ESAGASFLRRDGSVWSTDKDGIILALLAAELTAVTGQDPGEHFEALTAEFGMPYYTRI
                     DAAATPEQKARLQKLSPEDVREAHLAGEPITAKLTRAPGNNAAIGGLKVVSAHGWFAA
                     RPSGTENIYKIYAESFKGPDHLEAIVREARQIVDNALNQRA"
     gene            complement(1821..2861)
                     /locus_tag="PLU99_06030"
     CDS             complement(1821..2861)
                     /locus_tag="PLU99_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO16653.1"
                     /translation="MAKESFEILLKEFHRSKQLILEEISRHIVGQRDVVEQMLAAILT
                     RGHCLLVGVPGLAKTLMVSTLARITSLSFKRIQFTPDLMPSDITGTNVIDELPDGRRE
                     FRFVRGPVFANIVLADEINRTPPKTQAALLQAMQEREVTSGSQTWPLPNPFFVIATQN
                     PIEQEGTYPLPEAQLDRFMFSVWVDYPSLGEEQQILARTTGAELPPLRTVMSDRQVLN
                     MQKLIAAIPATEYVIDYAAAIVRASRPSDATAPDFVKKYVDWGAGPRAGQFLIWAGKA
                     MAAMDARYSVSIADIRKMAIPVLRHRIACNFAAQAAGLDSVKIIQKLIEHVPEPEIPR
                     YETKPPAEVVQE"
     gene            complement(2869..3429)
                     /locus_tag="PLU99_06035"
     CDS             complement(2869..3429)
                     /locus_tag="PLU99_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOO16654.1"
                     /translation="MPAMDENDNAAFGRFLRRVWRDDVTPLLVGENAAQRRTAAQVGG
                     KLAAATGLLLDGVLRLRGRPFTRSLTVLGTTLGAMLPDVWDWKWLREQAGPRQRQVVS
                     EQVQRRAAELPLLEALALFHLSPQAAQDDLKRAWRDISLRWHPDKAPDEAARAEYHVR
                     FLVYRSAYDRLRAAYEAGELPISAES"
     gene            3525..5159
                     /locus_tag="PLU99_06040"
     CDS             3525..5159
                     /locus_tag="PLU99_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16655.1"
                     /translation="MNEVLATLCLAEPVWRLSAAWIICGLAVLAGLGGRRRLPAGVSE
                     GLFVAGLSGILLAAYLPMRDPERSVVQLSAVLTALAVLWRFAPQRAGGGTGAAGLDSR
                     PPLSELEKVSRPPFAAGVVRSYAVLLCGAAVAGLSLRVSPDAPREQRLAVTLCLVLML
                     ALALRPRSGWASGVAWYAPLGLLACALRIWVLPYAAGWPANVMTMLVCAGGVLSLGEA
                     LVPVLAYWRNRRRGWQLQTERLLEPPPPWRLWWTGVQVLAGLTGIAALVVRDAPLAAF
                     GPALAAIGASVAGFRQRAVAGGSLALVLASEAAILATLAWLPDFGFNALAGATLAGLW
                     MIWLSRFWTQQLHEGQAWTYTGHLVGPARRIGCAAAVVCLALAVSEVAGPAAVAAISV
                     PQMIWSGVTFLLLVRLARMLGRDAGQGENWLGPFAACFALLAALVPLEPVVATLTRSW
                     PTWPVLLAVGLLLMALLGSRKRGRAEVQDWLYDAHIGGFLPVAIVCAGWTAGAGPFWF
                     GILAVALVAFGLRWPRSDRPDQSTAPPSLSASGCNL"
     gene            5253..6707
                     /locus_tag="PLU99_06045"
     CDS             5253..6707
                     /locus_tag="PLU99_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16656.1"
                     /translation="MPERECELRSIAWGEVFPFLRLFRTFRLAISPGRILTALAAVAL
                     VCLVGWVMDRIWVGAGHGALIDPATGQSEVAAYASMGSKDFALWQEHVKEMHEPAAAG
                     NFASLLRYEMRCFAAAVRGVMTLRWGFSAPAWDPQPSFLGSIVSAGQGVQWLVTQRPL
                     FFIIYAILHFAIFALFGGAICRSAVVEVARDESISPTNALRFAGQKFGGLALGPALPA
                     GIFAGILIVFIVVSFLGLIPWLGELLAGILFWLALAAGFALAMILIGTVLGLHLTWPT
                     IAAEGSDGFDAVSHAFAYVTQRPWYTAFYSALLLLYGAVCFVMVRLIALVTLKLTHAC
                     TNWGMSFFGAVNSARTDTVSKLDAMWHMPAWNDLTFLPTSGGVPFWGTFFAAPLDWSE
                     SIGAALIAFWVFIVVGLVGAFVVSFFFCGSTQMYLLLRRNVDAVDYDEIYYEEPEPKL
                     PAAAPGAAEPSTPGPTSQPAADETSAGSEPGGGA"
     gene            6750..7640
                     /locus_tag="PLU99_06050"
     CDS             6750..7640
                     /locus_tag="PLU99_06050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOO16657.1"
                     /translation="MPVERAEFRKLREAGAPPGISRAIGLRFVAWRDALARKLIRAGM
                     TPNRLTVLGFLMTAGAGCCLAVGASQQVPYLQGAAGPVGWSPVLAGLFLVLAGACDML
                     DGAVARLGNMSTRFGQILDSVLDRFSDMAIYVGCALHFTWQGNVTYQLLAVLALCHAC
                     LISYVKARSENLIPDCSVGYWMRGERIAAVLIGCATGHVPAVLWQQAISPFFTVVRRL
                     SYAGRVVQAMDTHEALPAAGPVPGWRGRLQLWRHPRGSISYDLVTGFNIAYIVLAARF
                     WPALQASGDWADPLARLLGR"
     gene            8214..8966
                     /locus_tag="PLU99_06055"
     CDS             8214..8966
                     /locus_tag="PLU99_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOO16658.1"
                     /translation="MIYGVYQSAAGMMANQYRQDVIANNIANADTVGFKRELATFAER
                     PPESVSGPRIGPSDPNLAGLTGGLLLARTETDFRPGSLVTTENPTDLALDGPGFFVVQ
                     VNGQPQYTRDGRFIAGADGVLRAASDGAPAVGVGGSQIVLNPYGGQVVVDEAGRVFQN
                     NALHGQLALADFADYSVLQHTEAGRFTAGDVEPSGAIACVRSGVLENSGVEPVRELVN
                     MIDASRAFELNARMLGLQDQTTGKLIGCLDRH"
     gene            8979..9779
                     /gene="flgG"
                     /locus_tag="PLU99_06060"
     CDS             8979..9779
                     /gene="flgG"
                     /locus_tag="PLU99_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOO16659.1"
                     /translation="MAVTALYTAATGMQAMSTKIDVIANNLANSDTVGYKASRANFQD
                     LLYQIYDLPGAQTSSETTAPLGTQIGLGTTLANTQILFQQGSAQQTGRSLDVMIQGDG
                     FFRVELPGGGIAYTRYGNFVRNADGELVLGNDFGNRLADDITIPTDIPVDRIGIGSNG
                     EVTVLDPDNNMTIVGQLQLARFPNPEGLIQFGSNLYLQSPATGEPIEGNPGDEGFGIL
                     SGGNLEMSTVEAVSELVELIKAQRVFQMNSQVIQASDETLRTVAALRV"
     gene            9817..10890
                     /gene="flgA"
                     /locus_tag="PLU99_06065"
     CDS             9817..10890
                     /gene="flgA"
                     /locus_tag="PLU99_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOO16660.1"
                     /translation="MSRRYRIVRYAVLVLAGAAARLSPAEGLANPIDLKIRAQSSIQS
                     GAVRLADVLVIPPGAEDALPANIRQAAVAAEVQGPATLVISYDQIAARLSELGVNSGR
                     VLLGGAAKCVVKVSAPQNAPEVRQCAAVVGSNGVPAERTLAQALREHITAELSALGGS
                     PEIEYERAGEPFLALTTPPWEFSIRSTNRNKLGPREFQVLLRRDGKNVRTATIMARVQ
                     LSKEVLVARAPLNVGTLVQREALALEPRVFQREQDLGLGSFEQAVGQRVAKFIAAGQM
                     VTAGDLKSEQLVQRSRPVTILGGSGNLTVRLAGVALDSGQYGETVRVRIGDSRQRRNV
                     LHGRVTGISTVRIEEGRQCLAYP"
     gene            10872..11708
                     /locus_tag="PLU99_06070"
     CDS             10872..11708
                     /locus_tag="PLU99_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOO16661.1"
                     /translation="MLSVPVSRIAARLPWMLGAAAALIWLSGASALAQHNSLLGLRAN
                     HAGGNQCRTAAATQPAAACDSFVARPANGIVLPPARSQARAANCIEPPPNPVLLAVSP
                     IAFEPPQPKVIEVNSLVTIIIRESRTSLNDAKMKSEKDWQTQATLARWFRLNPEDHLV
                     AQTFDPAPGVDWDFQNTYEGKGKYDRKDELTTRITARVIDVKPNGNLVLEARKSIKTG
                     DEVLLATLTGECRSEDVTAANTVLSTQIADLEIEMPDKGAVRDATTRGWLMRLFDFAK
                     PI"
     gene            11714..12832
                     /locus_tag="PLU99_06075"
     CDS             11714..12832
                     /locus_tag="PLU99_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOO16662.1"
                     /translation="MVLRQAAAIAVLVFLAPVARGEIRVQDIARLQGQRTNKLMGYGL
                     VVGLNGTGDGDKYSSTMRALANMHKRYQAPVLSDADLKGNNSVAIVAVEVSVPEFGAR
                     EGETLDVVVSVIGKAKSLAGGQLLTTPLQYAMLDPKDPATQQIYALAGGGIELPDPLA
                     PTRGVIRNGATLEADFFYAFIEDGAITLVLDDSHAGWTWSQMVARAINHAESEMALPT
                     VVDAAGGRRVVYLDTATALGPKNVRVRIPSAELPSPAGFISRVLETELFMLPEQQARV
                     TINRTSKSIAFTGTVTISPTVLQIPGFGTVSINGEGGAAAGPAGLVGLDTDKVGGARF
                     EELLQTLSALRVSPEQMVAAIEQLHKTGTLHAQLRYEE"
     gene            12861..13205
                     /locus_tag="PLU99_06080"
     CDS             12861..13205
                     /locus_tag="PLU99_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16663.1"
                     /translation="MNAVGAAGVLDLAAMQRLGRPIDAQNPATARLAATQMLSELFFK
                     PLLAEARESWLGAPFIDGGQTESIFGERLDQKLADVVAGQQTGLIDEIAGELTRTSWH
                     TRLQAGIKGKSE"
     gene            13358..13837
                     /gene="flgN"
                     /locus_tag="PLU99_06085"
     CDS             13358..13837
                     /gene="flgN"
                     /locus_tag="PLU99_06085"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOO16664.1"
                     /translation="MSDNLGALHGLLKQLVEQAEAKLAGLRTADSQALQACAARETEL
                     LQQVARNAQERQMILARVAQELRPPLRPDAPLAELIERFPEPQASILRARSLALRESA
                     AQLQGKNRLAARVAHHLQTHLRAIFSELAKAAAESVVYGPKGQHEQTRLRSCLDAVG"
     gene            13840..>14243
                     /locus_tag="PLU99_06090"
     CDS             13840..>14243
                     /locus_tag="PLU99_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16665.1"
                     /translation="MALLGASFQIGRSALAAYQSAIAITGQNIANVGNPDYARLSGRL
                     TALEGGMTLSGIAPGGGVNLSGLQRHVDAAVEARLRMSASLRAGSQATYDVLSQVESL
                     YNELSNYDLSTQLSTFFNGFSELQNDPDEITAR"
BASE COUNT         2368 a         4386 c         4895 g         2594 t
ORIGIN      
        1 tccttcgtcc tccgcctctc gtctccttcg tcctccgcct ctcgtctcct tcgtctcccg
       61 cctgtcggcc gatatcctta gatgactgga acgcacggtg aatcggagaa tcagcagcgg
      121 aaagacacta tggcgatttc gactctcgca ggtaagcctg ccccgaagca gcttctcgtg
      181 gacctggccg ctctcgaacg agaatactac gagcgcaagc ccgacttagg cgatcccaat
      241 caactggtca tcttcggaac cagcggccac cggggctcgc cgttgcgcgg cacgttcacc
      301 gaggcgcaca tctgcgccat cacgcaggcc atctgcgatt accgccgcag ccagggaatc
      361 gacggcccgc tctacatggg caaggacacg cacgccctct ccggcccggc ccaacgcact
      421 gcgctggagg tcctggcggc caacggcgtc gaaaccatca tccagcgcga cgacggcgtg
      481 acgcccacgc cggtcatttc gcgggccatc ctcgcccata accgcggccg ccaggaacac
      541 ctggccgacg gcatcgtcgt gacgccttcg cataacccgc ccgaagacgg cggcttcaag
      601 tacaacccgc cggatggcgg cccggccgac accagcgtta cgcgctgcat cgaagagcgc
      661 gccaaccagt tgctgcggcc aaacaacgcc ggcgtcaaac gcctgccgct ggccgccgcc
      721 atgaaagcgg acaccacgca cgaaacggac ctcatcacgc cttacgtgca ggatctgcgg
      781 aacgtgatcg acatggacgc gatcagcgcc gccggcctga gtctggccgt cgacccgctc
      841 ggcggcgcgg ccctgccgta ctgggcgccg atcaacgccg tttaccacct cgacatcacg
      901 gtggtaaacc cgaagctcga tccgaccttc tcattcatga ccgtcgacca cgacggcaaa
      961 atccgcatgg attgctccag cccctacgcc atggcccggc tggtcaacct gaaggatcaa
     1021 taccgcgtcg cgttcgccaa tgacccggac gccgaccggc acggcatcgt cacgccctcc
     1081 gcggccctga tgaaccccaa tcactacctg gctgtcgcga tccaatacct gctcacccat
     1141 cgcccgtact ggccacagca ggcggcgatc ggcaaaacgc tcgtcagcag cagcatgatc
     1201 gaccgggtgg tggagaagct gggccgccgg ctgttcgaga cgccggtcgg cttcaaatgg
     1261 ttcagcccgg ggctgttcga cgcgtcattg tgcttcggcg gcgaggaaag cgccggcgcc
     1321 agcttcctgc gccgcgacgg cagcgtctgg tccaccgaca aggacggcat aatcctggcc
     1381 ctgctggcgg ccgagcttac cgctgtcacc ggccaggatc cgggcgagca cttcgaggcc
     1441 ctgacggcgg agttcgggat gccctattac acgcggatcg acgccgcggc cacgcccgag
     1501 cagaaggccc gcctgcagaa gctgtcgccc gaggacgtgc gggaggcgca ccttgccggt
     1561 gaaccgatca cggccaagct gacgcgcgcc ccgggcaaca acgccgcgat cggtggattg
     1621 aaggtcgtga gcgcgcacgg ctggtttgcg gcccggcctt ccggcactga gaacatctac
     1681 aagatatatg cggagagctt taaaggcccg gaccatctgg aggcgatcgt gcgcgaagcc
     1741 cggcagatcg tggataatgc gctgaaccag cgcgcgtaac gcacgggagc aggcgacagg
     1801 ccttgcggcc gcagtctcga ctattcctgc acgacttccg ccggcggctt ggtttcgtag
     1861 cgcgggattt ccggctccgg cacgtgctcg atcagcttct ggatgatctt gaccgagtcg
     1921 agcccggcgg cctgggccgc gaagttgcag gcgatgcggt gccgcagcac cgggatggcc
     1981 atcttgcgga tgtcggcgat gctgacgctg tagcgggcat ccatggcggc catggccttg
     2041 ccggcccaga tgaggaactg ccctgcccgc ggtccggcgc cccagtcgac gtactttttc
     2101 acgaaatccg gcgcggttgc gtcgctcggg cgcgaggcgc gaacgatcgc ggccgcgtag
     2161 tcgattacgt actccgtggc cggaatggcc gcgatcagct tctgcatgtt cagcacctgc
     2221 cgatcgctca tcaccgtccg cagcgggggc agttcggcgc ctgtggtccg cgccaaaatc
     2281 tgctgctcct cgccgagcga gggataatcg acccacacgc tgaacataaa gcggtcgagc
     2341 tgcgcctcgg gcagcggata cgtgccctcc tgctcgatcg ggttttgcgt ggcaatgacg
     2401 aagaacgggt tcggcaaggg ccaggtctgc gagccgctgg tcacctcgcg ctcctgcatg
     2461 gcctgaagca aggcggcctg cgtcttgggc ggcgtgcggt tgatctcgtc ggccagcacg
     2521 atgttcgcga acaccggccc gcgcacaaag cggaactcgc gccggccgtc gggtagctca
     2581 tcgatcacgt tcgtgccggt gatgtccgag ggcatcaagt ccggcgtgaa ttgaatgcgt
     2641 ttgaaggaca gcgaggtaat ccgcgccagg gttgaaacca tcagcgtctt ggccagcccc
     2701 ggcacgccga ccagcagaca atgcccgcgc gtcagaatgg cggccaacat ctgttcgacc
     2761 acgtccctct gcccgacgat atgccgcgag atttcctcca gaatgagctg cttggagcgg
     2821 tgaaattctt tgagcaggat ttcgaacgac tctttggcca taaagctatc agctctcagc
     2881 agatattggc agctcgccgg cttcgtaagc ggcacgcagt cgatcgtacg cggaccggta
     2941 cacgaggaaa cggacatgat actcagcccg ggcggcctcg tcaggagcct tgtccgggtg
     3001 ccaacgcagc gagatgtcgc gccaggcgcg tttcaggtcg tcctgcgccg cctgaggcga
     3061 taagtgaaac aaagccagag cttccagcag cggcaattcg gccgcgcggc gttggacctg
     3121 ctcgctgacg acctggcgct gccgcggccc ggcttgctcg cgcagccatt tccaatccca
     3181 cacgtcgggc agcatcgcac cgagcgtggt cccgagtacc gtcagtgagc gcgtgaaggg
     3241 gcggccacgc aggcgcagca cgccgtccag caggaggccg gttgcggccg caagcttccc
     3301 gcccacctgg gcggcggtgc gacgctgggc ggcattctcg cctaccagaa gcggcgtaac
     3361 gtcgtcccgc cagactcgcc gcaggaaccg gccaaacgcc gcattatcgt tttcatccat
     3421 tgcaggcatc gcgtccctgc cgcaaacttt aggtccacgg ctgccctcgc cagcggtttt
     3481 ataccgcgct gagccaccgg cggcagccaa gagtggagtt cctggtgaac gaagttttgg
     3541 cgactctctg cctggcagag ccggtctggc gcttgagtgc ggcttggata atttgtgggc
     3601 tggcggtgtt ggccgggctg ggcggacggc gccgattgcc ggccggggtt agcgaggggc
     3661 tgtttgtcgc cgggttaagc gggattctgt tggccgctta tttgccgatg cgcgatcctg
     3721 agcgctcggt agtgcagctc agcgcagtac tgacggcgct ggcggttttg tggcggttcg
     3781 cgccgcagcg ggcgggcggc ggtacgggcg cggcggggct ggattcccga ccgccgttgt
     3841 ctgagctgga aaaggtttcg cggccgcctt ttgcagcagg cgtcgtcagg tcatatgccg
     3901 tgctgctgtg cggcgcggcg gtcgcggggc tttcgttgcg cgtctcgccg gatgcgccgc
     3961 gcgagcaacg cctggccgtg acgctctgtt tggtgctgat gctggcgctg gcgttgcggc
     4021 cgcggtcggg gtgggcctcc ggggtcgcgt ggtatgcgcc gttgggtctg ctggcttgcg
     4081 cgctgcgaat atgggtgctg ccgtatgcgg cgggctggcc ggcaaacgtg atgacgatgc
     4141 tcgtatgtgc gggcggagtc ttgtcgctgg gagaggcgtt ggtgccggtt ctcgcctatt
     4201 ggcgcaaccg gcggcgcggg tggcaactac aaaccgaacg cttgctggag ccgccgccgc
     4261 cgtggcgcct ctggtggacc ggcgtgcagg ttttggccgg gctgaccgga attgcggcgc
     4321 tggtggtccg cgatgccccc ctggctgcgt tcggccccgc cttggctgcg atcggcgcga
     4381 gcgttgcggg atttcgtcag cgggccgttg cgggcgggag cctggcctta gttctggcga
     4441 gtgaggcagc cattttggcg acgctggcgt ggctgccgga tttcgggttc aacgccttgg
     4501 cgggcgcgac gctggccggc ctgtggatga tctggctgtc gcgcttctgg acgcaacaac
     4561 tgcacgaggg ccaggcatgg acgtatacgg ggcatctcgt ggggccggcg cggcgcattg
     4621 ggtgtgccgc ggcggtagtc tgcttggcgc tggcagttag tgaggtggct gggccggcgg
     4681 cagtggccgc gatatccgtt ccgcaaatga tctggtcggg cgtgactttc ttgctgctgg
     4741 tgcggcttgc ccggatgctg ggtcgggatg cgggccaggg ggagaactgg ctcggtccgt
     4801 tcgcggcgtg tttcgccttg ctggcggcgc tcgtgccgct cgagccggtc gtcgccacac
     4861 tgacgcggtc ctggccgacg tggccagtgc tgctggcggt gggcctgctg ctgatggcgt
     4921 tgttgggcag ccgcaagcgt ggtcgagccg aggtgcaaga ttggctgtac gacgcgcaca
     4981 tcggcggttt cttgccggtg gcgattgtgt gcgctggttg gaccgccggc gcggggccgt
     5041 tctggttcgg gatattggct gtcgcattgg tcgcgttcgg tctgagatgg ccgcgcagcg
     5101 accggccgga ccagtcgacg gcgccgccaa gtttgagcgc cagcggatgt aatctataat
     5161 gccgccgcga tttgtctggg cgccaccccc gaacgcttgg cctggcgggg ggctgacaac
     5221 ggcgctgcgc ggcgtccaag aggagatgag ttatgcccga acgcgagtgt gaactgcgga
     5281 gcatagcctg gggtgaggtg tttccgtttc tgcgcctgtt ccgcacgttc cggctggcga
     5341 tcagtcccgg tcgcattctg actgcgctgg cggctgtggc gttagtgtgc ctggtgggct
     5401 gggtgatgga ccggatttgg gtcggcgccg ggcacggcgc gctcatcgac ccggccaccg
     5461 gccaatcaga ggtggcggcg tacgccagca tgggatcgaa agacttcgcg ctttggcagg
     5521 agcatgtcaa agaaatgcac gagccggccg cggccggcaa ctttgcttcg ctgctgcgct
     5581 atgagatgcg ctgcttcgcg gccgccgtgc gcggggtgat gactttgcgc tggggctttt
     5641 cggccccggc gtgggatccg cagccgtcct tccttggcag cattgtttcc gcgggccagg
     5701 gcgtgcagtg gctggtcacg cagcgtcctt tgttcttcat catttacgca attctgcact
     5761 tcgcgatttt cgcgttgttc ggtggcgcga tatgccgcag tgcggtagtc gaggtggcgc
     5821 gggatgagtc tatttctccg actaatgcgc tgcgcttcgc cgggcagaag ttcggagggc
     5881 tggcattggg cccggcactg ccggcgggaa ttttcgccgg catcctgata gtgtttattg
     5941 tggtgtcatt tctggggctg ataccctggc tgggtgagtt gctcgcgggg atcctcttct
     6001 ggctcgctct ggcggccggc tttgcactgg caatgattct tattggcacg gtgctggggc
     6061 tgcacctgac ctggccgacc attgcggccg aaggcagcga tggatttgac gcggtctcgc
     6121 acgcgttcgc ctatgtcacg caacggccct ggtatacggc tttctatagc gcgctgctgt
     6181 tgctctatgg ggcggtgtgc ttcgtaatgg tacggctgat cgcgctcgtc acgctgaagc
     6241 tgacccatgc ctgcacgaac tggggcatga gcttcttcgg cgccgtcaac agcgcgcgta
     6301 cggataccgt cagcaagctg gacgccatgt ggcatatgcc ggcctggaac gatctgacgt
     6361 tcctgccaac aagcggcggc gttccgttct ggggcacgtt tttcgcggcg ccgctggact
     6421 ggagtgagtc gattggtgcg gcgctgatcg cgttctgggt gtttattgtg gtcggcctgg
     6481 ttggggcctt tgtcgtcagc ttcttcttct gcggcagcac gcagatgtac ctgctactgc
     6541 gacgcaacgt ggacgccgtc gattacgacg aaatttacta cgaggaaccc gagcccaagc
     6601 tgccggccgc cgctccggga gccgccgaac cgtccacgcc cggcccgacg tcacaaccgg
     6661 ctgcagatga gacctcggcc ggcagcgaac ccggcggtgg tgcgtagttc tctatgcgga
     6721 acgacagtac gtccacagaa tgcccgaccg tgccggttga acgggcggag tttcgcaagc
     6781 tgcgcgaagc gggcgcacct ccgggcatca gtcgtgcaat aggcttgcgc ttcgtggcct
     6841 ggcgcgatgc tctggcgcga aagctgattc gcgccggaat gacgccgaat cgcctcacag
     6901 tcctgggctt tttgatgact gccggggccg gctgctgcct ggcggtcggg gcgtcgcagc
     6961 aagtgcccta tttgcaaggt gcggccggcc cggtcggctg gagcccagtg ttggccgggc
     7021 tgtttctggt cctggccggc gcctgcgaca tgctggatgg ggccgtcgcg cggctgggca
     7081 acatgagcac gcgcttcggc caaatattgg actcggtcct tgaccggttc agcgacatgg
     7141 cgatttacgt cggatgcgcg ttgcacttca cctggcaggg gaatgtgacg taccagttgc
     7201 tggcggtgtt ggcgctgtgt cacgcgtgcc tgatcagtta tgtcaaagcc cggtccgaga
     7261 acctcattcc ggattgttcc gttggctact ggatgcgggg ggagcgcatc gcggccgtgt
     7321 tgattggctg tgcgaccggg cacgtgccgg cggtattgtg gcagcaggcc atttcgccat
     7381 tctttacggt cgtgcggcga ttgagctatg cgggacgagt cgtgcaggcg atggacacgc
     7441 acgaggcgtt gccggcggcc ggcccggtgc ccgggtggcg cgggcgtttg cagctctggc
     7501 gtcacccgcg cggttccatc tcttacgacc tagtgacggg gtttaacatt gcgtacatcg
     7561 ttctcgcggc ccggttctgg ccggcgttgc aggcaagcgg tgactgggcg gatccgctgg
     7621 cgcggcttct ggggcggtag acgcgatctc tcgcagcgcg cttccgtcgt gttgggcggg
     7681 aagcgtttga catgcttcaa ttgcgccgct cgcgcaaaag ccgcggcgcc cgatccgagc
     7741 gcactatgcg gcaacggact ccccgatagc cgcttccgtg cagcgcaact ctttgccaga
     7801 tagagactta gcgaaaggcc cgtgcagcca ctcgcgcgct acgaactggg cgtggcatac
     7861 cccctgccct tgtcggcccc ctgatgccac gcgtttggtg cgtagggacg ccaccgcgcg
     7921 cggcagcgtt gccctaggag tcgtggtatg ccacggagag aatctctcct gcctccgaca
     7981 gcgcggcggc ctcttgcaga cgatgccgct tccaagtcgc tgctgccgga cgctgactcc
     8041 cagccgcggg attggagcga gatggggcgg cgggtgctgg ctgaggccca gcggcgagcc
     8101 acggatgagc tcatacgccg cggagccgtg cccgcgcccg ccgcctcgca acttccctca
     8161 ggcgtcctgc gaccggcacg cgtatctccc ccacttgcgc cggaggtcag ttcatgatct
     8221 acggcgtcta tcaatccgcg gccggcatga tggccaatca gtaccggcag gacgtcatcg
     8281 ccaacaacat tgccaacgcg gacaccgttg gctttaagcg agagctcgcg acattcgcgg
     8341 agcgaccgcc cgagagcgtg tccggaccgc gcatcggccc gagtgatccg aacctggccg
     8401 gcctgacggg cgggctgctg ctggcccgca cggagaccga ctttcggccg ggcagcctgg
     8461 tcaccaccga aaacccgacg gacctggctt tggacggccc cggcttcttc gtcgtgcagg
     8521 tgaacgggca gccgcaatat acgcgcgatg gacggttcat cgccggagcg gacggcgtgc
     8581 tgcgcgccgc cagcgacggc gcgccggccg tgggcgttgg cggatcgcaa atcgtgctca
     8641 acccctatgg cggtcaagtg gtcgtcgacg aggcaggccg tgtgtttcag aacaacgctc
     8701 tacacgggca actggcgctg gccgacttcg cggactacag cgtgctgcaa catacggagg
     8761 cgggccgttt caccgccggc gatgttgaac ccagcggcgc gattgcgtgc gtgcgctccg
     8821 gggtgcttga gaactcgggc gtcgagccgg tgcgggaact ggtgaacatg atcgacgcgt
     8881 cgcgggcgtt cgagctgaac gcgcgcatgc tgggcttgca ggaccaaacc acagggaaat
     8941 tgatcggctg ccttgaccgt cactaggagc cttgagcgat ggccgtaacc gcactctaca
     9001 ccgctgccac gggcatgcag gcgatgtcca ccaagattga cgtcatcgcc aacaatctgg
     9061 ctaactcgga cacggtcggg tacaaggctt cgcgcgccaa tttccaggac ctgctgtacc
     9121 agatttacga tctgcccggg gctcagacga gctcagagac taccgccccg ctggggactc
     9181 agattggtct gggcaccacg ctggcgaaca cgcagattct tttccagcag ggttcggcgc
     9241 agcagacggg gcgctcgctg gacgtcatga tccaggggga tggtttcttc cgcgtcgaac
     9301 tgccgggcgg cggcatcgct tacacgcgct atggcaactt cgtccgcaat gcggatggcg
     9361 aactcgtgct gggcaatgac ttcggcaatc gcctggcgga tgacattacc attccgaccg
     9421 acatcccggt cgacagaatc ggaatcggca gcaacggtga agtcaccgtg ctggatccgg
     9481 acaacaacat gaccatcgtc ggccagctac aactggcgcg gttcccaaac cccgaagggc
     9541 tcattcagtt cggctcgaat ctgtacttgc agagcccagc caccggtgag ccgatcgagg
     9601 gcaacccggg cgacgagggc ttcggcatcc ttagcggcgg caacctcgag atgagcacgg
     9661 tggaagccgt aagcgaactg gtcgagttga tcaaagcgca gcgcgtcttc cagatgaact
     9721 cgcaggtcat tcaggcctcc gacgagacgt tgcgcacggt tgccgcactg cgggtgtaga
     9781 cgtgattgaa gaccggatga cgcagaggat ttctcaatga gcaggcgata ccggatagtc
     9841 cgttacgcgg tgctggtgct ggccggcgca gccgcgcgac tgagcccggc tgaagggctc
     9901 gccaatccga tcgacttgaa aatcagggcg cagagttcaa ttcaaagcgg cgccgtcagg
     9961 ctggcggacg tgcttgtgat tccgcccggc gcggaagacg ctttgcctgc gaatatccgg
    10021 caggcggcgg tggccgcaga agtgcagggt ccggccacgc tggttatcag ctatgaccag
    10081 atcgccgctc ggctgagcga attgggcgtc aattccggcc gggtgctgct tggcggcgcg
    10141 gccaaatgcg tggtgaaagt aagcgcgccg cagaacgcgc cagaggttcg acaatgcgcc
    10201 gccgtcgtgg gctccaacgg cgtgccggca gagcggactc tggcccaggc attgcgcgag
    10261 cacattacgg cagaactcag cgctctcggc ggaagcccgg aaatcgagta cgagcgggcc
    10321 ggcgaaccat ttttggcgct gaccactccg ccttgggagt tttcgattcg ttcgacgaac
    10381 cgtaacaagc tcgggccgcg cgagttccag gttctcctgc gacgtgacgg caagaacgtg
    10441 cgtacggcca cgatcatggc ccgtgtgcag ctctcgaagg aagtgctggt ggcacgagcg
    10501 ccgttgaacg tcggcacgct ggtgcaacgc gaggcgctgg cgctggagcc gcgcgtcttt
    10561 caacgcgagc aggacctggg cctgggaagc ttcgagcagg ccgtggggca gcgcgtggcg
    10621 aaattcattg ccgccggcca gatggtcacc gccggcgacc tgaagagcga acaactcgtg
    10681 cagcgttcgc ggccggtgac gattctgggg ggcagcggca atctgacggt gcggctggcg
    10741 ggcgtggccc tggattcggg gcaatacggc gagacggtgc gtgttcgcat cggtgattcg
    10801 cggcagcgcc gcaacgtgct gcacggcagg gttacgggga tttccacggt tcgtattgag
    10861 gaggggcgcc agtgcttagc gtacccgtga gtcgtattgc cgcgcggctg ccctggatgc
    10921 tgggcgcggc agcggcgttg atctggctga gcggcgcgtc tgcgctggcg cagcacaact
    10981 cgctgctggg gctgcgggcg aatcatgcgg ggggcaatca gtgtcggacc gcagcggcta
    11041 cacagcccgc ggcggcgtgc gattcgttcg tggcccggcc ggcgaatggc attgtgttgc
    11101 cgccggcccg gtcccaggcg cgggcggcca actgcatcga gccgccgccc aacccggtgc
    11161 tgctggcggt ctcgccgatc gccttcgaac cgccgcagcc caaagtgatc gaagtaaaca
    11221 gtctggtgac gatcatcatt cgcgaaagtc gcaccagcct gaacgatgcc aagatgaagt
    11281 ccgagaagga ctggcagacg caggcgactt tggcgcgctg gttccggctg aatccggaag
    11341 accacctggt ggctcagacc ttcgaccccg caccgggcgt ggattgggat ttccagaata
    11401 cctacgaagg caaggggaag tacgaccgca aggatgagct gaccacgcgc attaccgccc
    11461 gcgttatcga cgtcaagccc aacggcaacc tggtgctgga agcccgcaag agcatcaaga
    11521 cgggggacga ggttctgctg gccacgctga ccggcgagtg ccgcagcgag gacgtcacgg
    11581 ccgcgaacac ggtgctcagc acgcagatcg ccgacctgga gatcgagatg cccgacaagg
    11641 gggccgtgcg tgacgccacg acgcgcggct ggctgatgcg tttgttcgat tttgccaaac
    11701 ccatttgagg cttatggtgc tacgtcaagc ggcagcaata gcagttctgg tgttcctggc
    11761 gccggtggcg cgcggcgaaa tccgcgttca ggatatcgcc cgcctgcagg gccagcgcac
    11821 caacaagctc atgggctacg gcctggtggt cgggcttaac ggcaccggcg acggtgacaa
    11881 gtattcctcg accatgcgcg ccctggcgaa catgcacaag cgctaccagg cgccggtact
    11941 cagcgacgcc gacttgaaag gcaataacag cgttgccatt gtggccgttg aagtgagcgt
    12001 ccccgagttc ggcgcccgcg agggcgagac gctggacgta gtggtttcgg tcatcggcaa
    12061 ggccaagagc ctggccggcg ggcagctttt gaccactccg ctgcaatacg ccatgctgga
    12121 tccgaaggac cccgccacgc agcaaatcta cgccctggcc ggtggaggca tcgagctacc
    12181 cgatccgctg gcgccgacgc gcggcgtgat tcgcaacggc gcgacgctcg aggcggactt
    12241 cttctacgcc ttcattgagg acggggcaat cacgctggtg ttggacgatt cacacgccgg
    12301 ctggacctgg tctcagatgg tggcgcgggc catcaatcat gctgaaagcg aaatggcgct
    12361 gccgacggtg gttgacgccg ccggcgggcg gcgcgtggtc tacctcgaca ccgccacggc
    12421 cctcgggccg aagaacgtgc gcgtgcgcat tcccagtgct gaactgccga gtcccgccgg
    12481 cttcatcagc cgcgtgctgg agacggagtt gttcatgctg ccggagcaac aggcgcgggt
    12541 gacgatcaac cgcacgagca agagtattgc ctttacgggg acggtaacga tctcgcccac
    12601 cgtgctgcag attccgggat ttggaacggt gtcgatcaat ggcgaaggcg gcgcggcggc
    12661 cggcccggcg gggctggtcg gcttggacac ggacaaggta ggcggggccc gttttgagga
    12721 gctgctgcag acgttgtcgg cgctgcgggt ttcgccggag cagatggtcg cggcgatcga
    12781 gcagcttcac aagacgggca cgctgcacgc ccaactgcgg tacgaggagt agcgggcaaa
    12841 tggcgcaagg aattcgacct gtgaacgcgg ttggcgcggc gggcgttttg gacctggctg
    12901 cgatgcaacg gttggggcgc cccattgatg cacaaaatcc ggctacggct cggttggcgg
    12961 ccacgcaaat gctgagcgag ttgttcttca agcccctgct ggcggaagcg cgcgaatcgt
    13021 ggctgggtgc gccgttcatc gacggtgggc aaacggagtc catttttggt gagcggctcg
    13081 accagaagct ggcggacgtc gtggcggggc agcaaaccgg cttgatcgat gagatcgcgg
    13141 gggaattgac gcggacttcc tggcacacgc ggctgcaggc tgggattaag gggaagtcgg
    13201 aatgaaagag cggaggccgg gcgcgttgac cggagatttg ggacgagtgg cgcggattgg
    13261 gggggcgaca cgggacgcgg cgcgcggcgc ggattccggg ccggcacggc tcggctctgc
    13321 tacgggctgt gttatgggcc ctgctacggt tgcggcgttg agtgataact tgggggcgct
    13381 gcatggactg ctgaaacaac tcgtcgagca ggccgaggcc aagctcgcgg gcttgcgcac
    13441 ggctgactcg caggcgctgc aggcgtgtgc cgcccgggaa actgagttgc tgcagcaggt
    13501 agcgcgcaac gcgcaagaac ggcagatgat ccttgcgcga gtggcgcaag agctgcggcc
    13561 gccactgcgg ccggatgcgc ccctcgcgga gctgatcgaa agatttcctg agccgcaggc
    13621 ctcgatttta cgggcccgta gcctggcgct gcgcgaatcg gccgcacaac tgcagggaaa
    13681 aaaccggctt gcggcgcgcg tggcacacca tctgcaaaca cacctccgtg cgatcttcag
    13741 cgaattggca aaggccgcgg cggagtcagt ggtgtacgga ccaaaaggac aacacgaaca
    13801 gaccagactg cggtcttgtc ttgatgcggt aggataagcg tggcactgtt aggggcttca
    13861 ttccagattg gtcgcagcgc gctggccgcg tatcagtcgg cgatcgccat caccgggcag
    13921 aacatcgcga acgtcggcaa ccccgattac gcgcgtctgt ccggccggct caccgcactg
    13981 gaaggcggca tgacgctaag cggcatcgcg ccgggtgggg gcgtgaacct gtcgggcctg
    14041 caacgccacg tggatgcggc ggtcgaggcc cgcctgcgga tgtccgccag tctgcgagcc
    14101 ggcagccagg cgacgtacga cgtcctcagc caggtcgagt cgctctacaa cgagctttcg
    14161 aactacgacc tgtccacgca gctttcgact ttcttcaacg gcttttccga actgcagaat
    14221 gacccggacg aaatcaccgc ccg
//
LOCUS       DAOSYS010000071        14119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3630_length_14119_cov_4.402019, whole genome shotgun sequence.
ACCESSION   DAOSYS010000071 DAOSYS010000000
VERSION     DAOSYS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3630_length_14119_cov_4.402019"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..456)
                     /locus_tag="PLU99_06095"
     CDS             complement(100..456)
                     /locus_tag="PLU99_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16666.1"
                     /translation="MSLSSSYQIKHAVISVAEAGDNTLVAAVAGKRICVVSMVLCPTE
                     SNSIYLHDDAAAPVALLGDASNKIQLDPKYGDGVPALVLPPHEGGWFETSIGQSLLIN
                     LSAAQGVAGCLTYALI"
     gene            complement(467..862)
                     /locus_tag="PLU99_06100"
     CDS             complement(467..862)
                     /locus_tag="PLU99_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16667.1"
                     /translation="MAGTIGTQTFDLIAGAEPPPKLNLVVEELERPGIDGTQISTVGK
                     RGAPFTWARCIIWRSGDTGWADCLQFYNELRALEGTLVSITDVQGNTTHNVLVRRVSA
                     QGPRAVFRDGALAAQMIFSVEMRMVAEEE"
     gene            complement(866..2353)
                     /locus_tag="PLU99_06105"
     CDS             complement(866..2353)
                     /locus_tag="PLU99_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16668.1"
                     /translation="MAGVVVEMTGDEARLWRAYQRIITQQVRMEQHGKTGAKRVKTET
                     DGLVTSLHSLVSRYASIGSIIGGFVSLSQILSEVQREGEAAAQQLGTVVEEARKLAQI
                     SAGGAPEIMAGMKAAQLMALQSGMSVAGAQNMVFMARTLGLSPEDAAQVARARLIGKS
                     DESVAGFMTAVGKFKGSEMFGAGAGTAEQIMSALLAAGGPVPADQLGTAAIKAAGSMR
                     MVGGDLDELLGMMSAISGGFASPDEAAGAIKALASVVEQKGFGGKGLMAGLQEFRAAR
                     PKEFEAAIGGSARLARGWQVISDAEAEGQIAQRVEDVRAARESGELYRSQLRAVQGVP
                     QLSLMQRRAQARLMHEIAGEQLGEKDVAYDTALDLYKTRSIMRGESPFVRALEVGISR
                     LQRYFRGSEYGERKLRETMYLEREMRGESRLVRPHGIPHPMPAEAPPTSFFGQELSQE
                     ITQLLSRQEALLRQIAESNRQMAENDRRGLTSRDGLANSSPDYVE"
     gene            complement(2472..3212)
                     /locus_tag="PLU99_06110"
     CDS             complement(2472..3212)
                     /locus_tag="PLU99_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16669.1"
                     /translation="MGAFLYWAPGDQLPEGLEHLDGLRLTQAGVLRSPDGASKPGVVL
                     ADEAHLAGQRLGLYPDEQTWRRVPGCQAWVGFYRAARPTPADLQRPTLLAGHAVRLAD
                     GQEWIAPIARGCVDDEAELRCYVALPRRSELDDDGRWVPGPVAERYGKLWECAVRFWD
                     AFVGAKPLATTPSDESGAPTSLSLEFTELHEAATAALAANYWVSAPELALLGALDEGT
                     AVAVCQALIDWPTIARWLEKKFPRPAAT"
     gene            complement(3222..4121)
                     /locus_tag="PLU99_06115"
     CDS             complement(3222..4121)
                     /locus_tag="PLU99_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16670.1"
                     /translation="MDRWNIYGASLGSTKIGAITQCEYRTGTETGGEPVSGSVHPVTQ
                     YILAQKPGASLTTLALSTALGAISQLGVGLHDAPLVLYYIKHEHGGARDVSGHKSVTF
                     RDGLLVPRQLSCAHGADADLSLEAFATYDGVNEPLIFSTAALPTGFDDAQRYGLGPVL
                     VGNVPLTGVRQVDIDFGLTVEPEGGNGEVWDRFVSVVEAKPVVTLRGIDLNWLGATTF
                     PLGGRIALHTNTSIYFRRRATGGTYVSDATAAHVRVSCSGLAYVERGGGDGGSAAENT
                     LTMPLFHDGTNLPLVINAGVIVS"
     gene            complement(4137..4631)
                     /locus_tag="PLU99_06120"
     CDS             complement(4137..4631)
                     /locus_tag="PLU99_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16671.1"
                     /translation="MVLIKFRKIGSPDLAARELRQYLREVWRMAGERWHQTMRPQHFT
                     RAGARKYGYLPRAGERGNERRNFRSSYTGRKLRRFGHTNPLVYTGLSMALTRPRDVRA
                     TAKGVRVVMNSPGFNRRNPRSRINMREELTRVTEEEANLLARLMEERLVQMLAGCRVT
                     TVEG"
     gene            complement(4638..5255)
                     /locus_tag="PLU99_06125"
     CDS             complement(4638..5255)
                     /locus_tag="PLU99_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16672.1"
                     /translation="MPTSQFATEITMGSLRISSRATRSQSGLITHELTSDTGLLGGQA
                     GTLTTYTDESNGVATLGADHGITQSSLVDVFWPGGLRYGMTAATVSGPDVTLSGGSGD
                     DLPSQYTEVIVSPRTELVTAFAGDDVDAIAAWSDTRMHLLFADSTDAALYARELVAGE
                     DWHWITGRGLANPLAGMDVAKLFVSVGAATPGELKLGVQYDSTPA"
     gene            complement(5264..5785)
                     /locus_tag="PLU99_06130"
     CDS             complement(5264..5785)
                     /locus_tag="PLU99_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16673.1"
                     /translation="MTPIGPISLAQSHLRQMLADCPAFRTWASAADVTAALARIHQES
                     LPPPAEHASEHTLAELHNYRPYALIWTAAENGLRLRADAASAEYEESGRLMIQLIQDV
                     PADLAADPAALDMAFKNSVGQIMADLCAHSGQAGYLCIRELGLAFGPYRTHPDDLPSL
                     GDAIAVELIVDWG"
     assembly_gap    5899..5908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5909..6090)
                     /locus_tag="PLU99_06135"
     CDS             complement(<5909..6090)
                     /locus_tag="PLU99_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16674.1"
                     /translation="MLHLAQWSSESAAIGGIAVTAVGALAVVCKRLSRNGQSRDCLKP
                     VDCARERPILQVGELLV"
     gene            complement(6102..6482)
                     /locus_tag="PLU99_06140"
     CDS             complement(6102..6482)
                     /locus_tag="PLU99_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16675.1"
                     /translation="MSALDDQMSLYGAPLLRDTLGRGVTYHAADGSAVPLAAAVVGPE
                     RAIEATSQQGERRVEYERSVTIDTDPALLTGGVADPAINAQVEVEGVRYAIAAVTRLA
                     GGRALLRCMRRAAAEAARRGYRGK"
     gene            complement(6485..7495)
                     /locus_tag="PLU99_06145"
     CDS             complement(6485..7495)
                     /locus_tag="PLU99_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16676.1"
                     /translation="MALPSSAVTRLDLSLTFSEFSLAANRRGFIGLRALPAVGVAQDS
                     AKFLKIPVEQLLKPVEDLKRSDKAGYKRDDFEFTEDSYATDDYGAEEVLGDRSLKRYS
                     SLIRAERIHAMRAIYRVQQAYEQACANAVFDTATWTGEALTTNVDIPWTTAASAKPIE
                     DIDGAIDQVTANIGRRPNGIIIPYKAAKAMMRTAQITDLVKYSGHTDPTQLVASAVAI
                     LQSLFDFEIILIPDGVKNTGKEGGSAAFTRHWDPTMCMVCNVAQDNGDLEDPEPGIGR
                     TIMWEEENASIPGAGEAELPIIMDEYREEHRRGSILRARADFQAKIIYPEAGHLLTGL
                     TA"
     gene            complement(7557..8753)
                     /locus_tag="PLU99_06150"
     CDS             complement(7557..8753)
                     /locus_tag="PLU99_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16677.1"
                     /translation="MIVQKDIVNFPVDPTAAMAPYLRVVLSEGYLVAAGAEDHDLGTL
                     TERVLAGATVAAVEPPTLNGTVKMVADGAIAQYARVYGAAGGKVSAAQNANYIGLALT
                     AASDDGNIIEVMRLPRLDGTHVVSLPVNSELAAGLRVVLSGGYLVAAGAEQPEIGTLV
                     DATTSEDTVASVVSPVVASMLHGSVNVVAAGAVTQYADVYGAADGKVSTTRNSHYIGV
                     AVTAAAKDGDVIEVMRLPQRGRLDYVNTSDSAEVENVGTETAFDVSKTLDGAALKTGD
                     VLHIVAAGTVVDNNSTDKLTIKLYVGTEEIVATGAVDVADNDVWYIDAWVVVRDVGVN
                     GKLAACGVQALGVPGTVTAKPFSKAQATEDLSDEVTVQVTATWSAKHEDNEVKLDHLI
                     VERIPA"
     gene            complement(8780..10120)
                     /locus_tag="PLU99_06155"
     CDS             complement(8780..10120)
                     /locus_tag="PLU99_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOO16678.1"
                     /translation="MNQTDLCVAWGQRLGHVLAVYEPPFSEYLADVRAHAPAGQCPTQ
                     FAQDRKLGQPHYQMERGNVGVIRISGVLSKYPSVHQYYFGGTAATVAQKSIEMAAADD
                     SVRAIALLVDSPGGSSAGIADLADAIYAARQRKRVIAYASDLMASAAYMVASQADVIY
                     GNLSAMVGSIGTYGVIYDWSAYFAEAGVKVHVIRAGQFKGAGTFGTEITPEQLSEWQR
                     EIDQLNAVFLGIVGRGRPQMAAKLDKLADGRVHVGPAAVNAGLIDGLKDWNAVLAELG
                     DESSARVVGRSSVAGATAEETTMANADAPAGPAPVTLAELTKALPDLSADQKLYCLEH
                     GYTLEQATQYALDQKRLELQQREAEIKRLKAAAASPGVQPLGTRGDAETSAQEVDGDP
                     VAKFDELVAQHMQRNGGDRWAAVASVSSAHPELHQAYLSATANTCGKTRRVGRR"
     gene            complement(10107..10466)
                     /locus_tag="PLU99_06160"
     CDS             complement(10107..10466)
                     /locus_tag="PLU99_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16679.1"
                     /translation="MRANKLILCLLLTLTGCTAASLSSRAGRGPGSATPDAPPASPRD
                     ELLQQAAGTIAANIATELSGIRQSTTNAYDPWTLRLQTLWPMVLAVPLLTYIAPKVGW
                     RAVARLAQRWERRRESD"
     gene            complement(10466..11029)
                     /locus_tag="PLU99_06165"
     CDS             complement(10466..11029)
                     /locus_tag="PLU99_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16680.1"
                     /translation="MTRVITLSVSVVIVLCGCAASHWGGEAAGKNPGVHVRGGSLVSP
                     PEASFTGDGGGRLGKLTYSDGGKDGPLTLTVQDVEFTQAASSVVEREPAKIRAIADLQ
                     RVQVEYVQASWAGMVSLAHELAPILKLLAMAQFTDSEAGLTMTLPSGVSVGGQKLSTP
                     AELQRFFSEALQTVEATAAAAPPVVQQ"
     gene            complement(11026..12645)
                     /locus_tag="PLU99_06170"
     CDS             complement(11026..12645)
                     /locus_tag="PLU99_06170"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOO16681.1"
                     /translation="MRRRRDGSDFRPTEALREIRADYDAANESRYMPALTGVLPSGSG
                     QDYHYRYESHYWRMMERARAADRNNMVIGQGITRLVDNVLQDGFTPDPETGFAKLDAA
                     IKARRQNWAEDPEQCDAAGEFTHHQQARLTLRAVIVDGDILHLPLQAGSLQAVEAHRL
                     RTPRNRTKRCIVHGVVLDRTTRRRLAYLLTKDEVDPLAPLRSFSEVSEYPARDRAGNR
                     MVLHIYDPKRVSQTRGVTALAPIMYPLGMHEDLQFANLLRAQVAACYAVFHEFSAEVA
                     LSAPDQRGERMDEILSDGGRRRIEGIAPGMEVWGRPGETLKGFSPNVPNPEFFQHAAL
                     TLSIIAINLDLPVQIFLLDPTRTNFSGWRGAIDQARLGMRRIQNWMIARFYRPVYTWK
                     LRQWLASDQEIRARATEAGIDPDNPPLAVFRHRWQRPRWPYIEPNKDISALSARKRSL
                     LAAPSDVAGDDGYDYEDLVERIVRDNTLLIETAAARARELSSKLGVEILPQTVLFPTE
                     QWAWAASRGGGNRPERPGADETSTGGNGAAA"
     gene            complement(12648..12947)
                     /locus_tag="PLU99_06175"
     CDS             complement(12648..12947)
                     /locus_tag="PLU99_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16682.1"
                     /translation="MPTISATSTEAEIRAAYLDNCGYAEAGSVAMAKRFVTVLRAMQM
                     RGITAVEQEGQKLEYDLATLRAQEQAALRFIAECATGAGGEAPRIIHADLRHFRR"
     gene            complement(13070..13816)
                     /locus_tag="PLU99_06180"
     CDS             complement(13070..13816)
                     /locus_tag="PLU99_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOO16683.1"
                     /translation="MGADTSIAWTDHTYNPIWGCTRVSDGCRHCYAAALARRMGYQVW
                     GPSATRRVFGDKHWAEPVTWDRSAQRTHTRRRVFVGSMCDWCDPHPAMTAARPRLWAL
                     IRQCSHLDWLLLTKRAERIEGCLPADWGEGYANVWLGTSVENNDYAWRAETLALIPAA
                     VRFVSCEPALGPLDRLRLDGIDWVIYGGESGPGYRAHDPTWARDLLARCRQHPDRPAF
                     FFKQSSARRPGSGVMLDGQIIHEYPVPRTP"
BASE COUNT         2285 a         4846 c         4361 g         2617 t
ORIGIN      
        1 gtgcgcgagc agttccgccg gctcgatggt gatgctgcgg ccgtccgagg cgaccagcga
       61 gagcgtaacg ccagactgtg cgcgtatgtt gggcatggat cagatgagcg cgtacgtcag
      121 gcagcccgcc acgccctgcg ccgccgacag gttgatcagc agtgactgcc cgatactggt
      181 ctcgaaccaa ccgccttcgt gcggcggcaa gaccagtgct gggacgccgt cgccgtactt
      241 ggggtccaac tgaatcttgt tgctcgcgtc gcccaacaac gccaccggcg cagcggcatc
      301 atcgtgcaaa tagatcgaat tcgactcggt tgggcaaagc accatactaa ccacgcagat
      361 gcgtttgccc gcgaccgcag ccacgagcgt gttatcgcct gcctcggcga cgctgatgac
      421 ggcgtgcttt atctggtaac tgctactcaa gctcattgcg aagctcctat tcctcttctg
      481 cgaccatgcg catttcgacc gagaagatca tttgggccgc cagcgcgccg tcacggaaca
      541 ccgcgcgcgg cccctgagcg gacactcgac gtaccaatac gttgtgcgtg gtgttaccct
      601 gaacgtccgt gattgagacc agcgtgccct cgagggcgcg cagctcgttg taaaattgca
      661 ggcagtccgc ccagcccgta tcgccgctac gccagatgat gcagcgcgcc cacgtgaacg
      721 gcgcgccgcg cttgcctact gtgcttattt gtgtcccgtc aatacccggg cgctccagtt
      781 cctcaaccac taggttcagc ttcggcggcg gttccgcgcc ggcgatcagg tcgaacgttt
      841 gcgttccgat cgtgcccgcc atcgcctact ccacataatc cgggctgctg ttggccagcc
      901 cgtcgcggct ggtcagaccg cgccggtcgt tttccgccat ctggcgattg ctctctgcga
      961 tctggcgcag cagcgcctcc tggcgcgaca gcaactgcgt gatctcttgc gacagctcct
     1021 ggccaaagaa cgaggtcggc ggcgcctctg ccggcatcgg gtgggggatg ccatgcggtc
     1081 tgaccagccg cgactcgccc cgcatctccc gctctaagta catcgtttcg cgcagtttgc
     1141 gctcaccgta ctctgagccg cggaaatacc tctggagccg ggaaatcccc acctctaacg
     1201 cccgcacgaa gggcgattcg ccgcgcatga tggagcgggt cttatataaa tccagcgccg
     1261 tgtcgtatgc gacgtctttc tcgcccagtt gctcgcctgc gatctcgtgc atgaggcgcg
     1321 cctgtgcgcg gcgctgcatg agactcaact gcggcacacc ctgcaccgcc ctcaactgcg
     1381 atcgatatag ctcacccgat tcacgcgcgg cgcgcacatc ctcgacgcgc tgcgcgatct
     1441 ggccctccgc ctccgcgtcc gaaatcacct gccagccccg ggccagacgg gcgctaccgc
     1501 ctatcgccgc ctcgaattct ttcggccggg cggcgcggaa ctcctggagc ccggccatca
     1561 ggcccttgcc gccaaagccc ttctgctcta ccacgctggc caacgccttg atggcacccg
     1621 cggcctcgtc tgggctcgca aagccgccgc tgatggctga catcatgccc agcagctcgt
     1681 ccaggtcgcc gcccaccatg cgcatcgacc ccgccgcctt gatcgccgcg gtgccgagtt
     1741 gatcggccgg cacggggccg ccggccgcta ggagggccga catgatctgc tcagccgtgc
     1801 cggcgccggc gccgaacatc tccgacccct tgaacttgcc gacagccgtc atgaagcctg
     1861 cgaccgactc atccgacttg ccgatcaggc gtgcccgcgc cacctgcgcg gcgtcctcgg
     1921 gactcagccc cagagtgcgg gccataaaga ccatgttttg cgcgccggcc acactcatgc
     1981 ctgattgcag ggccatgagt tgggcggcct tcataccggc catgatctcc ggggccccgc
     2041 ccgccgagat ttgggccagc ttccgcgcct cttcgaccac cgtgcccaac tgctgcgcgg
     2101 ccgcctcccc ctcccgttga acctccgaca ggatctggga cagcgacacg aagccgccga
     2161 tgatgctgcc gatgctggcg taccgagaga cgagcgagtg cagagaggtt accaggccgt
     2221 cggtttccgt ttttacgcgc ttggcgccgg tcttcccgtg ttgctccatc cgcacttgct
     2281 gggtgattat gcgctggtag gcccgccaca gccgggcctc gtcgccagtc atctcgacta
     2341 ctacgcctgc cataccatcc tcggcacgat catcacgggc ggctcagagc ccaggccggt
     2401 cgccagcgcc cacaggtccg ccatgctcgg tctatacgcg tggtgccggc cggccgccca
     2461 gcggcggtaa ctcaggtagc ggctggccgg gggaattttt tttcgagcca tctcgcaatc
     2521 gtcggccagt cgatcaacgc ctggcagacc gcgaccgcgg tgccctcgtc tagggcgcct
     2581 agcagtgcca gctcaggcgc cgacacccaa tagttggccg ccagggcggc cgtagcggcc
     2641 tcgtgcagct ccgtaaactc gaggctcagg gatgtggggg ccccggattc gtctgagggc
     2701 gtcgtcgcca gtggtttcgc gccgacaaac gcgtcccaaa agcgcaccgc acactcccac
     2761 agtttgccat accgctcggc gacgggtccc ggcacccagc ggccgtcgtc atccagctcg
     2821 ctgcggcgcg gcagtgcgac atagcaacgc agctccgcct catcgtccac gcagccgcgg
     2881 gcgatcgggg cgatccactc ctggccgtcg gccagccgga ccgcatggcc cgccagcagc
     2941 gtggggcgct gcaggtccgc tggagtcggc cgggccgcgc gatagaagcc gacccaggcc
     3001 tggcaacccg gcacacgccg ccacgtctgc tcgtcggggt atagcccgag gcgctggccg
     3061 gccaggtgcg cttcatcggc cagcacaacc ccaggcttgc tcgcgccgtc tggtgaccgc
     3121 agaacgcccg cctgggtcaa gcgcagaccg tctaggtgct ctagaccctc gggcagttgg
     3181 tcgccgggcg cccagtataa gaatgcaccc atgcgagcct cctagctgac gattacgccg
     3241 gcgttgatga ccagcggcag gtttgtgccg tcgtggaaca gcggcatagt cagcgtgttt
     3301 tcggccgcac tcccgccgtc gccgccgccg cgctcaacgt aggcgagtcc gctgcaactg
     3361 acacgcacgt gggcggccgt cgcgtccgat acgtacgtgc cccctgtggc acgccgccgg
     3421 aaatatatgg aggtgttcgt gtgcagcgca atccggccgc ccagcgggaa cgttgtggcg
     3481 cccaaccaat tgaggtcgat accgcgcaac gtcacgaccg gcttggcctc gaccaccgag
     3541 acgaaccggt cccacacttc gccgttgccg ccctcgggct cgaccgttag gccgaagtcg
     3601 atatccacct gccgcacacc agtcagcggc acgttaccca ccagcaccgg gcctagcccg
     3661 tatcgctgcg cgtcatcgaa ccccgtcggc aacgcggccg tgctgaagat cagcggctcg
     3721 ttgacgccgt cataggtcgc gaacgcctcc agcgacaggt cggcatcagc tccgtgggcg
     3781 caggacagtt gccgtggtac caacagcccg tcgcggaacg tcaccgactt gtggccgctg
     3841 acgtcgcgcg ctccgccgtg ttcgtgtttg atgtagtaga gcaccagcgg ggcatcgtgt
     3901 agccctacgc cgagctggct gatcgcaccc agggcggtcg agagcgccag tgtggtcagg
     3961 ctcgcgccgg gcttctgggc gagtatatat tgcgtcaccg ggtgcacact gcccgacacc
     4021 ggttcgcccc cggtctcggt accggtgcgg tactcgcact gcgtgatcgc accgattttc
     4081 gtgctgccca gtgatgcgcc gtagatgttc cagcggtcca tggctcgctc ctattctcac
     4141 ccctccactg ttgtgacgcg gcagccggcc agcatctgca cgagtcgctc ttccatgagc
     4201 cgcgccaaca gattggcctc ttcctccgtg acgcgggtca gctcctcgcg catgttgatc
     4261 cgactgcgcg gattgcgacg gttgaacccc ggcgagttca tgacaactcg cacccccttg
     4321 gccgtggccc gcacgtcacg cggccgcgtg agcgccatcg acagccctgt atagaccaga
     4381 gggttcgtgt ggccgaaccg gcgcagcttg cggccggtgt acgagctgcg gaagttgcgc
     4441 cgctcgttgc cgcgttcgcc ggcccgcggc agatagccgt acttgcgcgc cccagcgcgc
     4501 gtgaagtgct gcggccgcat ggtctgatgc caccgctcac ccgccatgcg ccagacctcg
     4561 cgcaggtact ggcggagttc gcgcgcggcc aggtcagggc tgccgatctt tcgaaacttg
     4621 atcaggacca tgtttcatca ggctggagtt gagtcgtact gtactcccaa cttcagttct
     4681 cccggcgtgg cggcgcccac gctcacaaac agtttcgcga catccatgcc cgccagtggg
     4741 ttcgccagac cccggcccgt gatccaatgc caatcctcgc cggcgaccaa ctcgcgcgca
     4801 tagagcgccg cgtcggtcga atcggcgaac agcaggtgca tgcgcgtatc agaccacgcc
     4861 gcgatcgcgt cgacgtcatc gccggcgaac gccgtgacca gctcggtacg cggactgacg
     4921 atcacctctg tgtactggga cggcaggtcg tcgccggatc cgccgctgag cgtcacatct
     4981 ggtcccgata cggtcgcggc cgtcatgccg taacgcagcc cgcccggcca gaacacgtcg
     5041 accaagctcg actgcgttat gccgtgatct gcccccagcg tggccacacc gttggactcg
     5101 tccgtatacg tcgtcagtgt cccggcctgg ccgcccaaca accccgtgtc actggtgagc
     5161 tcgtgcgtga tcagacccga ctggctccgt gtggcgcggc tgctgatacg caggctgccc
     5221 atggtaatct cggtggcgaa ctggctcgtt ggcatgatga ctcctaaccc cagtcgacta
     5281 tcaactcaac cgctatcgcg tctcccaggc tcggcagatc atccggatgc gtgcggtacg
     5341 gcccaaaagc caggcccagc tcgcggatgc acaggtagcc ggcctggccg ctatgcgcac
     5401 agaggtcggc catgatttgc ccgaccgaat tcttgaacgc catgtccagc gccgccggat
     5461 ccgcggccag atcggccggc acgtcctgga tcaactggat catcagccgg cccgactctt
     5521 cgtactcggc actggccgca tcggcccgca accgcaggcc gttttcggcc gcggtccaga
     5581 tcagtgcata cggccgatag ttgtgcagct ccgcgagtgt gtgctcggag gcgtgctccg
     5641 ccggcggcgg taggctctcc tggtggatgc gcgccagcgc ggcggtgacg tcggcagccg
     5701 acgcccaggt gcgaaacgcg gggcaatcgg ccagcatctg ccggaggtga gactgcgcca
     5761 gactgatcgg accaattggg gtcatttctt actgcgcatc acattgaaca actcgcgaat
     5821 ctcggcgtgc agctcgtcca ggcgccgggc cgcatccgcc tgccattgcc ggatctgacc
     5881 gaactcgcgc gcgcaatcnn nnnnnnnnac caggagctcc ccaacctgaa ggatcgggcg
     5941 ctcgcgcgcg caatcgaccg gcttgaggca gtcgcgactc tggccgttgc gactcaggcg
     6001 tttgcagacg acggccagcg cgccgactgc ggtgaccgct atcccgccga tcgccgcgct
     6061 ctccgaggac cattgcgcga ggtgcaacat accggtgtct cctacttgcc tcggtacccg
     6121 cgccgcgcgg cctcggcggc cgcgcgccgc atacaacgca gcagcgcccg gccgccggcc
     6181 aggcgcgtca cagccgcgat ggcgtagcgc acgccctcga cttcgacctg tgcgttgata
     6241 gctggatcag ctacgccgcc ggtcaacaac gcggggtcgg tgtcgatcgt gacgctgcgc
     6301 tcgtactcca cgcggcgctc cccctgctgg ctggtggcct cgatcgcccg ttcgggtccg
     6361 accaccgcag ccgccagcgg caccgcggag ccgtccgcgg catggtacgt caccccgcga
     6421 ccgagcgtgt cacgcagcag cggggcgccg tagagcgaca tctggtcatc cagcgcgctc
     6481 atccctacgc cgtgaggccg gtcaacagat ggccggcttc cggatagatg atcttcgcct
     6541 ggaagtcggc gcgggcgcgc aggatcgagc cgcgccggtg ctcctcccgg tactcatcca
     6601 tgatgatcgg cagctcggct tcgccggcgc cggggatgct ggcgttctcc tcctcccaca
     6661 tgattgtgcg gccgatgccc ggctccgggt cctcaaggtc gccattgtcc tgagcgacat
     6721 tgcacaccat gcacatcgtg ggatcccagt gccgtgtgaa cgccgcgcta ccgccctctt
     6781 tgccggtgtt cttcacgccg tccgggatga ggattatttc gaaatcaaac agcgactgta
     6841 ggatagcgac cgccgaggcg accagttgag tcggatcggt gtggccggag tatttgacca
     6901 agtcggtaat ctgcgcggtg cgcatcatcg ccttagcggc tttgtagggg atgatgatcc
     6961 cgttcggacg ccggccgatg ttggccgtga cctggtcgat cgcgccgtca atatcttcga
     7021 tcggcttcgc gctggccgcg gtggtccagg gtatgtccac gttcgtggtc aacgcctcgc
     7081 ccgtccaggt ggcagtgtca aacaccgcat tagcgcaggc ctgctcataa gcctgctgca
     7141 cgcgatagat cgcgcgcatc gcgtggatgc gctccgcccg aatcaggctc gagtatcgct
     7201 tcaatgagcg gtcgccgagg acctcttccg caccgtagtc gtccgtggcg tagctgtcct
     7261 cggtgaactc gaaatcatcc cgcttatatc cggccttgtc cgagcgtttc aggtcttcga
     7321 ccggtttcag caactgctca acgggaatct tgaggaattt cgccgagtcc tgcgccacac
     7381 cgacggccgg cagcgcgcgc agtccgatga atccgcgacg attggcggcc agagaaaact
     7441 cgctgaacgt gagcgagagg tccagcctcg tgactgctga ggatggtagt gccatgtttc
     7501 aggtctccat tcaggccgcc gccaccggcc aagaaaaagg ttactttcta ggccaactag
     7561 gccgggatgc gttcgacgat caggtgatcg agcttgactt cgttgtcctc gtgcttggcc
     7621 gaccaggtcg cggtcacctg cacggtgact tcgtccgaca ggtcctcggt ggcctgagcc
     7681 ttcgagaacg gcttcgccgt gacagtcccg ggcacgccca gggcctgcac accgcaggcg
     7741 gccagcttgc cgttcacgcc aacgtcacgg acgacgaccc aggcgtctat ataccagacg
     7801 tcattgtcgg ccacgtccac agcgcccgtc gcaacaatct cttccgtgcc gacgtacagc
     7861 ttgatcgtca gcttgtccgt ggagttgttg tcgacgactg tgccagccgc cacgatgtgg
     7921 aggacgtcac cagtcttgag cgccgcgccg tccagcgttt ttgagacgtc gaacgctgtc
     7981 tcggtgccga cattttccac ttccgccgaa tcgctggtat tgacgtagtc cagtcggcct
     8041 ctctgcggca gacgcatgac ctcgatgacg tcgccgtcct tggcggcagc ggtcacggcc
     8101 acgccgatgt agtgcgagtt ccgtgtggtg ctcacctttc cgtcggccgc gccatatacg
     8161 tcggcgtatt gggtgacggc cccagccgcg actacattga cgctgccgtg gagcatagag
     8221 gccaccaccg gagagaccac cgaggcaaca gtgtcttcgc tcgtcgtggc atccaccagc
     8281 gtcccgattt cgggctgctc ggccccggcc gcaacgaggt aaccgcctga cagcaccacc
     8341 cgcaggcccg cagcgagctc cgagttaacc ggcaggctga ctacatgcgt cccgtcgagg
     8401 cgcggcagac gcattacctc aatgatgttg ccgtcgtcgc tggcggcagt cagggcgagg
     8461 cctatgtagt tcgcgttctg cgcggccgac accttgccgc ccgcagcgcc gtatacgcgg
     8521 gcatattgcg caatggcacc gtccgcgacc atcttgacgg taccgtttag ggtcggcggc
     8581 tctaccgcgg cgacggtcgc gcctgcgagc acgcgctcag tgagcgtgcc taggtcatgg
     8641 tcttcggccc cggccgcgac gaggtaacct tcggacagca ccacgcgcag gtatggcgcc
     8701 atcgccgccg tcgggtcaac cgggaagttc acaatatcct tttgaacaat cattgttttc
     8761 gatcctttct tttagggggt tacctccgcc caacgcgacg cgttttgcca caggtgttcg
     8821 ccgtcgccga caggtacgcc tggtgcaact cgggatgggc gctcgagacg gacgcgacgg
     8881 ccgcccagcg atcgccgccg ttacgttgca tgtgctgggc gaccaactcg tcgaacttag
     8941 cgactgggtc gccgtccact tcctgtgcgg aagtctcagc gtcgccgcgt gtgcccaatg
     9001 gttgcacgcc cgggcttgcg gcggcggcct tgagccgttt gatttccgcc tcgcgctgct
     9061 gcaactcgag gcgcttctgg tcgagcgcgt actgggtcgc ctgttctagg gtgtacccgt
     9121 gttctaggca gtatagtttc tggtctgcgc tcaggtccgg cagagcctta gtgagctctg
     9181 ccaacgtaac cggcgccggt cccgccggcg catcagcatt agccatagtg gtctcctccg
     9241 cggtcgcccc ggcgaccgac gatctaccaa ctactcgcgc ggacgattcg tcgcccagct
     9301 ccgcgagtac tgcgttccaa tccttcagcc catcgatcag cccggcgtta accgcggcgg
     9361 ggccgacatg cacgcgcccg tctgccagct tgtccagctt cgcggccatt tgcggccggc
     9421 cgcggcccac gatccccagg aagactgcgt taagctgatc gatctcgcgc tgccactcag
     9481 agagctgctc gggcgtgatc tccgtgccaa acgtaccggc ccccttgaac tgacccgcgc
     9541 ggatcacgtg cacctttacg ccggcctcgg cgaaataggc cgaccaatcg tagatcacgc
     9601 cataggtgcc gatcgatcca accatggccg acaggttgcc gtagatcaca tctgcttgtg
     9661 aggcaaccat atatgccgcc gaggccatca ggtcgctcgc gtaggcgatg acccttttac
     9721 gttgccgcgc ggcatagatg gcgtcggcta gatcggcgat tccggccgag gagccaccag
     9781 gtgaatcgac cagtagcgcg atggcgcgca cgctatcgtc ggcggccgcc atctcgatcg
     9841 acttttgcgc gacagtcgct gcggtcccgc cgaagtaata ctggtggaca ctcgggtact
     9901 tgctcagcac gccggagatg cggatcacgc ccacattgcc gcgctccatc tggtagtgcg
     9961 gctgacccaa cttgcgatcc tgcgcgaatt gggtcggaca ttggccggcc ggcgcgtgcg
    10021 cgcgaacgtc tgctaggtac tccgagaatg gcggctcata gaccgccaac acatgcccca
    10081 gacgctggcc ccatgcgacg caaaggtcag tctgattcac gacgtctctc ccagcgctgg
    10141 gccagacggg ccaccgcccg ccagcccacc ttaggcgcta tgtaggtgag cagcggcact
    10201 gcgagcacca tcggccacaa agtctgcagg cgcagtgtcc agggatcata ggcattggtg
    10261 gtgctctgtc tgatgcccga tagttctgtg gcgatgttcg cagcaatcgt ccccgccgcc
    10321 tgttgcaata gctcgtcacg cggggaagcg ggaggcgcgt ccggagtggc ggaccccggg
    10381 ccgcgcccgg cccggctgct caggctggct gccgtacaac cagtgagcgt cagcaagagg
    10441 cagagaatga gtttgtttgc gcgcattatt gctgcaccac aggcggcgcc gccgcggccg
    10501 tcgcctcgac tgtctgcaag gcctcactga agaatcgctg taattcggcg ggcgtgctca
    10561 gcttctggcc cccgacactg acaccactcg gcagcgtcat agtcagacca gcttcagagt
    10621 ccgtgaactg cgccatggcg agcagcttga ggatcggtgc cagctcgtgc gccagtgaga
    10681 ccatgccggc ccaggaggcc tggacgtact ccacttgcac gcgctgcaga tctgcgatgg
    10741 ctcgtatctt ggccggctca cgctccacga ccgagctggc cgcttgcgtg aactcaacat
    10801 cctgcacagt gagcgtcagc ggtccgtctt tgcccccatc actatacgtc agcttaccga
    10861 gccggccgcc gccgtcgccg gtgaacgagg cctccggcgg cgagaccagc gagccgcctc
    10921 ggacgtggac gcccggattt ttccccgccg cctcgccacc ccagtggctc gccgcgcacc
    10981 cacaaagcac gattactact gatactgaga gggtaatcac acgtgtcatg ccgcagctcc
    11041 attgccccca gtgctcgttt cgtcggcgcc cgggcgctcc gggcgattgc ctccgccgcg
    11101 actggccgcc caggcccatt gctcggtggg gaacagcacg gtctgcggca gtatctctac
    11161 acccagcttg ctgctcagct cccgcgcccg ggccgccgcg gtctcaatta gaagcgtgtt
    11221 gtcgcgcaca atccgctcga ccaggtcctc atagtcgtag ccgtcatcgc cggcgacatc
    11281 cgacggcgcc gccagcagcg agcgcttacg cgccgacagc gcggaaatgt ccttattagg
    11341 ctcgatgtac ggccagcgtg gcctctgcca cctatgccgg aacactgcga gcggcggatt
    11401 atccggatca atgccggcct cagtggcccg ggcgcgtatc tcttgatcac tcgccagcca
    11461 ctggcgcagt ttccacgtgt agaccggccg gtagaaccgc gcgatcatcc agttctgtat
    11521 tcgccgcata cccaagcgcg cctggtcgat cgcaccgcgc cagccgctga aattcgtgcg
    11581 tgtcgggtct agcaaaaaga tctggacggg caggtcgaga ttgatagcaa tgatagacag
    11641 cgtcagcgcc gcgtgctgaa agaattctgg gttcggcacg ttcgggctga agccttttag
    11701 ggtttcgccc ggccggcccc agacctccat gccgggcgcg attccctcga tccggcgccg
    11761 gccgccgtca ctgaggatct catccatgcg ctcgccgcgc tggtcgggcg cgcttaatgc
    11821 gacctcggcg ctgaactcgt ggaataccgc gtagcaggcc gcgacctggg cccggagcag
    11881 attggcgaac tgaagatcct cgtgcatccc cagggggtac atgatcggcg cgagcgccgt
    11941 cacgccgcgg gtctggctga ccctcttagg gtcatatatg tgcagcacca tccgattgcc
    12001 cgcccggtcg cgggccgggt attcgctgac ctcgctaaaa ctgcgcaacg gagctagcgg
    12061 atcgacctca tctttggtca acaggtatgc caggcgtcta cgcgtggtgc gatcgagtac
    12121 tacgccgtgc acgatgcagc gcttggtgcg attgcgcggg gtgcggagcc ggtgcgcttc
    12181 gacggcctgg agcgagccgg cctgcaaggg caagtgcagg atgtcgccgt ccacgatcac
    12241 cgcccgcagc gtgaggcggg cctgctggtg atgggtgaac tcgccggccg cgtcacactg
    12301 ctcgggatcc tccgcccagt tctgccggcg cgccttgatg gccgcatcca atttcgcgaa
    12361 gccagtttcc gggtccggcg tgaacccgtc ctggagcacg ttgtcgacga gccgcgttat
    12421 gccctggccg atgaccatgt tgttgcggtc tgcggcccgg gcgcgctcca tcatgcgcca
    12481 gtagtggctc tcgtagcgat agtggtagtc ttggccactg ccgctcggga gcacgccagt
    12541 cagcgcgggc atgtagcggg attcgttcgc tgcgtcatag tcggcgcgga tttcccgcag
    12601 cgcctcggtc ggtcggaagt ctgagccgtc tcgccggcgg cgcatgctca cctccggaag
    12661 tgccgcaggt ccgcgtggat gatgcgtggg gcttcaccgc ccgcgcccgt ggcgcactca
    12721 gcgatgaatc gcagtgcggc ctgctcttgt gcgcgcagcg tcgccaggtc gtattccagc
    12781 ttttggccct cctgctctac agccgtgatc ccccgcatct gcatggcgcg cagcaccgtg
    12841 acaaagcgct ttgccatcgc cacactgccg gcctctgcgt agccgcagtt atcgagatag
    12901 gcggcgcgga tttctgcctc ggtggatgta gcgctaatcg ttggcacggc gcgcactatc
    12961 cacgcgcggc gctggcgttc aaggtgttag ctttcggatt cttcggattc ttcggatttg
    13021 aggcagcggg cgggctctga tcacgcccac cgcattgcca gcctagtcct cagggggtgc
    13081 gcgggaccgg gtactcgtga atgatttggc cgtccagcat caccccggaa cctggtcgtc
    13141 gcgcggagga ctgcttaaag aaaaacgccg gccggtcggg atgctgacga cagcgcgcca
    13201 ggaggtcgcg ggcccatgtt ggatcgtgcg cgcggtaccc tgggccgctt tcgccgccgt
    13261 agatgaccca gtctataccg tccagtcgta ggcgatccag cggtcctagg gccggctcgc
    13321 acgaaacaaa ccgcacggcg gccgggatca gggccaacgt ctcggcacgc caggcgtagt
    13381 cgttgttctc gaccgaggtg ccgagccaga cattcgcata gccctcgccc caatcggccg
    13441 ggagacaccc ctctatccgc tcggcgcgct tggtcaacag caaccaatct agatgcgagc
    13501 attgccggat cagtgcccac aaccgcggcc gcgcagcggt catcgcgggg tgcgggtcgc
    13561 accaatcgca catcgagccc acgaaaacac ggcgacgcgt gtgcgtgcgt tgcgcgctgc
    13621 ggtcccaggt tacaggctcc gcccagtgct tgtcgccaaa tacccggcgc gttgcgctcg
    13681 ggccccacac ctggtatccc atccggcgcg ccagggcggc ggcgtaacag tggcggcagc
    13741 cgtctgacac ccgcgtgcag ccccaaatag ggttataggt atggtcggtc caagcaattg
    13801 acgtgtcagc acccattttc atccatcctt cactttgtgt ccacacgctc cagtcgcggt
    13861 agttgcgcgc gtctctacgg cgtctattgc ctcctgcgca acctgcgccg cttttcggct
    13921 aatcgctttt gtgtctgccc cgctcgcctg tcagcggcgg gggtggggtt attcatgggc
    13981 ttctcctttc agcgcggcca gggcggcgcg gaggttgcca aacacgcacg ccctgtttgt
    14041 cttactgcaa tcgtggtcgc agtcctcgca ggctttcgtt gccgcctcaa tcagccccgc
    14101 cagcgcggcc gggtccagg
//
LOCUS       DAOSYS010000072        14046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3660_length_14046_cov_5.374384, whole genome shotgun sequence.
ACCESSION   DAOSYS010000072 DAOSYS010000000
VERSION     DAOSYS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3660_length_14046_cov_5.374384"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1315)
                     /locus_tag="PLU99_06185"
     CDS             complement(143..1315)
                     /locus_tag="PLU99_06185"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO16684.1"
                     /translation="MALIPIGTDLRLKKPPVGNYVLIALNVLVFLFTDFFGGAAGQLV
                     KHQWTLYAAVPDLGQYITYQFLHGDWMHLLGNMLFLWIFGNAVCDRMGSLCYVLFYLA
                     GGVIAGVTFAALNDNALLGASGAIAAVTTAFLVLYPRVKITMLLWFFLITAFQIDAII
                     LIVFKMLLWDNILAPTLDQGAIANVAYSAHLAGYAFGFVIAMSLVAAGVLPRSQFDLL
                     AFWSRWRRRVGLPGGADVVVRGRPITVQEVGSRPLEPLQLTPAERLREEAVDRAQARD
                     MANAAELYLELLRIDPNQVLPRDTQLDVANHFAQCQRHTEAAAAYEAFLSAYRSTPDV
                     HQVRLLLGLIYSRYLRQWQRAAEHLQAALGNLTHPEQRALAESELRHVQAQVQSEA"
     gene            complement(1532..2053)
                     /gene="coaD"
                     /locus_tag="PLU99_06190"
     CDS             complement(1532..2053)
                     /gene="coaD"
                     /locus_tag="PLU99_06190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOO16685.1"
                     /translation="MANSHEITAVYAGSFDPVTLGHLDVIRRAARLFNKLIVGVGRNP
                     DKSEVFTQSERIALLEPHLRELPNTQAQAYEGLTSEFVRNIGGSVLVRGIRDFTDLSF
                     ELLQANVNMAIGGVETVFLLTSDRHVLTSSTYIKQIFEMGGGRRRALVERLVPPNVAQ
                     ALEEKLGAGAGGD"
     gene            complement(2108..5113)
                     /locus_tag="PLU99_06195"
     CDS             complement(2108..5113)
                     /locus_tag="PLU99_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO16686.1"
                     /translation="MSGMQTADGLSSIHLMQPGYLAVLALLPLVVVLARRSLAGLGRV
                     RGGLTVCLRCLVIAVVSLALTSPEWVQTTDDQSVVFALDQSDSVPRAAREDAKQFIRA
                     ASAGMRPGKDRIALLGFDGRPSVEQICGPQLVADNVGAPIEPHRTNVAGALRLGLALL
                     PADTAKRIVVLSDGNENVGNVVEEAEICAALGIPIDVLPLRYQHASEILVDQFSAPVA
                     AKQDEVVSLRLVLRSQAATKARVVLYHNDRQVRDGAGPTGGFDVQLAAGPNRFTIPVA
                     LRAAGVHRFRAVVEPADPAADTLATNNEARAFTIVGPAERVLIISDTAMDPAQVNETS
                     AEVLARALQQAGIECVQMLVEELPADPAALADCSTVILSNVSAFALGETRQQALASYV
                     RDQGGGLIVVGGDQAFSVGGYGYTPLEQVLPVETARDKLQFLSLAMVIVIDRSGSMLG
                     EKITMACEAARASVQLLSSRDRVGVIAFHSEPEWAVRLQPAGDKNQIARQIDVIGAGG
                     GTDMYPALGEAFAALADVDSNLKHIIVLTDGQSAPGDFEGFASTCRAAGITISTIAVG
                     PDADRPLLNRIATLSGGRMYVAESARPLPQIFARETVLASRSGLYERQFTPRLQSVLN
                     NQILDGFTQADIPPLHGYVVSEAKPLSQTPLARITEDGTDPIMAYWQVGLGKAVAFTS
                     GLWPKWGPEWANWPGFSKLWTQAVRYAGRPGTSSELQLETGVKGGEGHAVVSAEHLPF
                     RWQGTLTLSGRLTRPDFSSEPLRLQRTAADRFEATFSAEAPGTYLVSVPYEYGAGDEG
                     GRGVLRGGLVVNYSAEYRTLRDNSGALAEIARRTGGRVLDVNDAGAVFETGSIRPVHV
                     RSPFWDDLVRLTLVLFLLDVAVRRIAVTPADAARRIRTFIREAVGRPAAEEAAATLAT
                     LRGVKGRKREEQSPGASESPKADEAAVRSEAPERQPSDSLSQALGGASDKPVVAAPPP
                     KRRPPGTTESEYTARLLRAKRRARDSREP"
     gene            5237..6316
                     /gene="purM"
                     /locus_tag="PLU99_06200"
     CDS             5237..6316
                     /gene="purM"
                     /locus_tag="PLU99_06200"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOO16687.1"
                     /translation="MKNGPNPTKGRTYREAGVDIDAADRFVDLIAPAVRSTYGPRVID
                     SHGAFAGLFRLDYDERLFAHNYRDPVLVACTDGVGSKLLVAIQARRVDTVGIDLVAMN
                     VNDLLCCGAEPLLFLDYVAVNKVEPEVLAQVVRGIVAGCQQAGCALLGGETAEMPDLY
                     RPGHFDLAGFAVGVVERRRMLGPRHVRPGDEVIGIASSGLHSNGYALARRVLLDDAGL
                     DPNSRVRGLDEPLVEALLRPTRIYVRPVLDVLRHYKRQRMVAAMAHITGSGLPGNIPR
                     ILPPNCDAVLRRGSWPVPPIFGLLEHHGVEEEEMYRVFNMGIGYVMIVRQQSAGVVIR
                     VLERAGEQAFVIGRVRRGRGQVEIR"
     gene            6316..7290
                     /locus_tag="PLU99_06205"
     CDS             6316..7290
                     /locus_tag="PLU99_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16688.1"
                     /translation="MPGHRLPRLALLASFAVLPVLTTGCAATRALPPSVAYQSEPWKF
                     GRHCGTKLTTAHYDIYTTLTDPQQIEMIPDLVERAYEFYQQLVPPVHEPAERMPVYLL
                     ATRNQWATATAHLTGARAEIFLRVRNGGFSDGGVSVIQYVEPSITGPLLAHEGFHQYL
                     HCCVGPAVPAWLNEGLAVVCEGQRWTATRLREFDPHFNPTRQSRLAEAVNRDRLFPLE
                     ELLRTHAGNVVQGTSTQVGTYYAQLWALMLFLKEGEDGKYAAGFERLRRELATPAAAQ
                     RLRAEQIWAEDGQVNPGEALFRSYICDDLPTFEREYVAYLQSRISAAY"
     gene            7695..9101
                     /locus_tag="PLU99_06210"
     CDS             7695..9101
                     /locus_tag="PLU99_06210"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOO16689.1"
                     /translation="MADVRIERDSMGEMEVPATARYGASTHRALLNFPISGYRFGRRF
                     IRALGLIKYAAAMANELLGRLDPVRARVIKEAAREVMDGKHDDQFVLDVFQTGSGTST
                     NTNANEVIAHRSNELLTHGEAAGPQSELRVHPNDHVNMGQSSNDVIPSAIHIAAVEAL
                     KADLMPALHRLHNALKAKAEEFDSIVKIGRTHLMDATPIRLGQEFSGYASQIEHADRR
                     LMQKIVTLRELPIGGTAVGTGLNTHPDFARRVCEIINHETRESFQEAANHFEAQHAKD
                     AVVAASGALKVLAISLTKIANDIRLLGSGPRCGIGELLLPTIQPGSSIMPGKVNPVIP
                     EAVLQVCAQVVGSDAAITYAAGLLGNLDLHVGMPVMAHNLLESIRLLANACNVFVDKC
                     VSGIQANRQRCEELIERSLAMCTSLAPVIGYDAAAAIAKEAHSTGKTVRQVASEKGIL
                     EPARLEELLDPRWMTHPA"
     gene            9281..9823
                     /locus_tag="PLU99_06215"
     CDS             9281..9823
                     /locus_tag="PLU99_06215"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOO16690.1"
                     /translation="MALQFRIVTTRLGELAFVFDERGLRRVLLPQPAGPRGLRAAVKR
                     EFPAAREDSRLAPRLAGQLQRYFDGERIEFDVQLNLEAESATEFRRAVWLACHQIPYG
                     QTTTYGDLARRVGHPRAARAVGTAMRSNPCPIIIPCHRVLGCNDCLGGYSAPGGIEFK
                     RRLLEMEGATFRTALKEEPV"
     gene            9969..10436
                     /locus_tag="PLU99_06220"
     CDS             9969..10436
                     /locus_tag="PLU99_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HOO16691.1"
                     /translation="MHRLVAPQFTPLLPQWRDAGVYQLWIRVNRPVVLRVGGLGRLHF
                     APGTYVYTGRAARGLRRRVLRHVRGAEHFHWHIDYLLASPAARVIRVNLAATDPAEEC
                     RVNRAAGCGALAPVQRFGASDCRSRCPAHLWLMMRGARAAAIDVALSGEKECK"
     gene            10433..11956
                     /locus_tag="PLU99_06225"
     CDS             10433..11956
                     /locus_tag="PLU99_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO16692.1"
                     /translation="MTVTTTLRTAADSEPARRKAPGWVIACVALYAAGVFLPFLGART
                     LTPHEVMVSHPALRILEDGHWIVPRFASGFWLDKPPLVNWLTAACFAIVGGFSEFAAR
                     LPAALSAVGLCVLMAVLAGRFFSTRVALLTGLVQASCVYMYMQGRLGEIDIVLALLLT
                     AAHGVLLWHWGRGGFDLPWRASLLFYFLAALGVLAKGPVAVALLGATVIAYAIWRRSW
                     RPIRAVLISPGVLVFVLVAGGWHLAAAYSVGAEALQEWNYNTVARFLGLHRLGSQSFF
                     LYFRDIPWMVLPWTVALVLGARVLAAGYRGPDGPVHRFLWSWFLGGLGFLLLALFKHK
                     HYAIPILPPLSILAALVIDAHLVKKGVHARRFYTIVFAVCLLGFAFVGGYVMPRQDHR
                     RVTAEFVAGATAGVPADETLYVIGLAQSSAYPYIAHKRCAYLDRLDDIRDELRERQRP
                     MWVLSLRKYAQLAADRGLSFEQAAAEQPRRKHPEPETLVLGRLWESEPAASSPAGPR"
     gene            12335..13345
                     /locus_tag="PLU99_06230"
     CDS             12335..13345
                     /locus_tag="PLU99_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOO16693.1"
                     /translation="MTASWTAESILNLARGYQEACILAAAAEFDLFTRLADGGQTAEE
                     LAAAVQADVRATRYVLDALTALELLEKTGQRYRVPEQLAPLVTSGGPGSVLPMLQHQA
                     ACVRRWAQLSWAMKTGRPVEAETIRGAEADREAFLEAMNCVSMPLLSGLIKALQPLRF
                     SHLLDVGGASGTWTIAFLRAAPEATATIFDLPDALPLAKRRLAAEGLANRIHLVGGDF
                     YEDPLPQGADLAWLGAIIHQNSREQNRALLAKVYEALAPGGQVVIRDVIMDPSRTRPV
                     MGALFAIMMLLATDAGGTYTFEEVSEDLSATGFIDATLVRRGEFMDSLIRATKPGQLP
                     AD"
     gene            13421..>14046
                     /locus_tag="PLU99_06235"
     CDS             13421..>14046
                     /locus_tag="PLU99_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16694.1"
                     /translation="MSALALLAPVFACLGSDWEPDAWAGDAEKGMVYMLPGIDGQPGS
                     LLEAYAGLRDGGVQAEIRVFNWGPYAMLNLADYVRNRAVARAIADEIVAFQDQYPEAP
                     VDLVGYSGGGALVLLVLESLPAERSVRKAVIVQAGVSPDYDLSEALWHVEDQLVSFYC
                     PSDWLVLGVLTELVGTVDRAHTASAGKDGFDTQRAIPDPSLADKLAQI"
BASE COUNT         2357 a         4608 c         4546 g         2535 t
ORIGIN      
        1 ctcggccccg aaggcggcga agccggcggc cgcatcgtgg cccaaggccc ccccgaagaa
       61 atcgccgcct gccccgagag ccacaccggg cgctacctgg ccggcaagct gcaatccgcc
      121 ccccagccat agaagccgct gcctaagcct cgctctgcac ttgagcctgc acgtgcctca
      181 gctcggactc cgccaaggca cgctgctcgg ggtgcgtcag attccccagg gccgcttgca
      241 ggtgctccgc cgctcgctgc cattgccgca gataacgcga atagatcagc cccaacagca
      301 gtcgcacctg gtgcacatcc ggtgtcgagc gataagcgct caaaaaagct tcgtacgccg
      361 cggccgcttc ggtgtgccgc tggcattggg cgaaatggtt ggccacgtct aactgcgtgt
      421 cgcgcggcag cacctggttc gggtcgatcc gcaacagctc caggtaaagc tcggccgcat
      481 tggccatatc gcgggcctgg gcgcggtcca ccgcctcctc ccgcagccgc tcggccggcg
      541 taagctgcag cggctccaaa ggccgcgagc ccacctcctg cactgtgatt ggccggccgc
      601 gcaccaccac gtcggccccg cccggcaggc cgacgcgccg ccgccagcgg ctccagaacg
      661 ccagcaggtc aaactgcgag cgcggcagca cgcctgcggc caccaagctc atcgcgataa
      721 caaagccgaa cgcgtagccc gccaggtgag cggaataggc cacgttggcg atcgcgccct
      781 ggtccagtgt gggggccaga atgttgtccc acaggagcat cttgaagacg atcaggatga
      841 tcgcgtcgat ctggaaggcg gttatcagga agaaccagag cagcatcgtg atcttgacgc
      901 gcggatacag caccagaaac gctgtggtca ccgccgcaat cgcgccggag gcccccagca
      961 gcgcgttgtc gttaagagcg gcgaacgtca cgcccgcgat gacaccgccg gccaggtaaa
     1021 agagcacgta gcacagcgag cccatgcggt cgcacacggc gttcccgaaa atccacagga
     1081 agagcatgtt gcccagcagg tgcatccagt cgccgtgcaa gaactggtac gtgatgtact
     1141 gccccaggtc cggcaccgcg gcataaagcg tccactggtg cttgactagc tgtcccgccg
     1201 cccccccgaa aaagtccgta aacaggaaaa cgaggacgtt cagcgctatc agcacgtagt
     1261 ttccgactgg cggcttcttc agtcgtaagt ccgtgccaat cggtattaac gccatccgct
     1321 ttccttccgg gataatacgc cgcaccaggc gagagggcga gactctgtta gcctgctctg
     1381 tcgccggatc agccgcaagc cgcgcccgct acacgcctac aacttgatcg gtaatatccc
     1441 gggcacgcgc tatttgctca gatcacacga ccaaagcacc gcttcgtcca gcagccacgc
     1501 aagcctgtgg ttttgcactc ctggatcgcc gctagtcccc gcccgcgccg gcacccaact
     1561 tctcctccaa agcttgagcg acattgggcg gaacgaggcg ctcgacgagg gcccgtcgcc
     1621 gaccgccgcc catctcgaaa atctgcttga tgtatgtgct tgacgtgagg acgtgccggt
     1681 cgctggtgag cagaaagacg gtctccacgc cgccgatggc catgttcacg ttggcctgca
     1741 gcagctcgaa actcaggtcc gtgaagtcgc gaatgccgcg caccaagacc gagccgccga
     1801 tgttccgcac aaactcggag gtcagccctt cgtaagcctg ggcctgcgtg ttgggcagct
     1861 cgcgcaaatg cggctcgagc aaggcgatcc gctcgctttg cgtgaaaacc tcgcttttgt
     1921 ccgggttgcg cccgacgccg acaatcagct tgttaaacag ccgcgctgcc cggcggatca
     1981 cgtccaggtg cccgagcgtg accgggtcga acgagccggc ataaacggcc gtgatttcgt
     2041 gtgaatttgc catgtttcca gccaatctta agcagcgtga tggcttgtcg ctagcaagcg
     2101 tcgttcctca aggttccctg gagtcacgag cccgccgctt ggcgcgcaac aagcgcgccg
     2161 tgtattcgct ttcggtcgtt cctgggggac gccgcttcgg gggcggggct gcaaccacag
     2221 gcttgtccga ggcgccgccc agggcctggg acaatgagtc gctcggttgc cgctccggcg
     2281 cctcgctccg gacggctgcc tcgtccgcct tggggctttc agacgctccg ggcgactgtt
     2341 cctcgcgttt ccggcccttc acgccgcgca gcgtggccag ggtagccgcg gcttcctcgg
     2401 cagcgggccg gccgacggcc tcccgtatga aggttcgaat gcgtcgggca gcgtccgcgg
     2461 gcgtcaccgc aatgcgccgg acggctacgt ccagaaggaa cagcaccaac gtcaggcgga
     2521 ccagatcgtc ccagaacggg cttcgcacgt gaacgggccg gatgctcccc gtctcgaata
     2581 cggcacccgc gtcgttcacg tccagcacac gtcctcccgt gcggcgcgca atctccgcga
     2641 gtgcaccgct attgtcacgc agtgtgcgat actcggccga atagttcaca accaatccgc
     2701 cgcgaagcac cccacgcccg ccctcgtcac ccgcgccgta ctcatacggc acactgacca
     2761 aatatgtccc gggcgcctcg gctgaaaacg tcgcctcgaa ccggtcggcg gccgtgcgct
     2821 gtagacgcag tggctcggaa gaaaaatccg gccgggtcaa ccgccccgag agcgtcagag
     2881 tgccttgcca gcgaaacggc agatgctccg ccgagacgac agcatggcct tcgccaccct
     2941 tgactccggt ttcgagttgc agttcggatg aagtgcccgg gcgcccggca tatcgcacgg
     3001 cttgcgtcca gagcttgctg aaccccggcc aatttgccca ttccgggccc cacttcggcc
     3061 aaagcccgct cgtaaatgcg accgccttgc ccaatccaac ctgccagtaa gccatgatcg
     3121 gatcggttcc gtcctcggta atccgcgcaa gcggcgtctg agacagcggt ttggcttcgc
     3181 ttaccacgta gccatgtagt ggcggaatat ccgcctgggt aaatccgtcc agaatctgat
     3241 tgttgagcac cgattgcagc cgcggggtaa actggcgttc gtacagcccc gaccggctgg
     3301 ccagcaccgt ttcgcgggcg aagatttgcg gcaacggccg agcactctcg gccacgtaca
     3361 tccgcccgcc gctgagtgtg gcgatccggt tcagcagtgg acggtcggcg tcgggcccca
     3421 cggcgatggt tgaaatggtg atgccggcag ctcggcaggt gctggcaaac ccctcgaaat
     3481 cccccggtgc cgactgtcca tcggtgagca cgatgatgtg cttcaggttg ctgtcgacgt
     3541 ccgccaaagc ggcaaacgcc tctcccagcg ccgggtacat atccgtgcca cccccggctc
     3601 cgatcacgtc aatctgccgg gcgatctggt tcttgtcgcc cgcgggttgc agccggaccg
     3661 cccattccgg ctcgctgtgg aacgctatga cgcccacccg atcacgactg ctcagcaatt
     3721 gcacggaggc gcgggccgcc tcgcacgcca tggtgatctt ttcgccgagc attgaccccg
     3781 agcggtcaat cacgatgacc atcgccaggc tgaggaattg aagcttgtcg cgggccgtct
     3841 ccaccggcag aacctgctcg agcggcgtat atccgtatcc gcccacgctg aacgcttgat
     3901 cgcctcccac cacgatcaga ccgccgccct gatcgcgcac gtatgacgcc agcgcctgct
     3961 gccgcgtttc ccccagcgcg aaggccgaca cgtttgacag gatcaccgtg gagcaatccg
     4021 ccagggccgc cgggtccgcc ggcagctcct cgaccagcat ctgcacgcac tcgattcccg
     4081 cttgctggag ggcccgcgcc agcacttcgg ctgacgtctc gttgacttgc gccggatcca
     4141 tcgccgtatc gctgattatg agcacacgct ccgcgggccc cacaatcgtg aacgctcggg
     4201 cttcattgtt cgttgccagc gtgtccgccg cggggtcagc cggttccacc accgcgcgaa
     4261 agcgatgtac accggctgcg cgcagcgcca ccggaatcgt gaaccggttg ggcccggccg
     4321 cgagttggac gtcaaacccg cccgttgggc ccgcgccatc ccggacttgg cggtcgttgt
     4381 gatacaggac cacgcgcgct ttcgtcgccg cctgcgaacg tagtacgagc cgcaacgaaa
     4441 cgacttcgtc ttgcttggca gcgaccgggg ccgaaaattg atctaccagg atttccgacg
     4501 cgtgctggta ccgaagtggc agtacgtcga ttgggatgcc gagcgcggcg cagatctccg
     4561 cttcttccac gacgttgcca acgttctcat tgccgtctga aaggaccacg atccgcttgg
     4621 ccgtgtctgc cggcagcagc gccaacccca gccgcagggc gccggcgaca ttcgtccggt
     4681 gcggttcgat tggagcgccg acgttatccg ccaccaactg cggaccgcag atctgttcca
     4741 cggacggccg gccatcgaag cccagcaggg cgatccggtc cttgccaggc cgcatgccgg
     4801 cgcttgccgc gcggataaac tgtttggcat cctcgcgcgc ggcgcgcgga acgctgtcgg
     4861 actggtccag ggcgaacaca acgctctggt cgtcggtcgt ctgcacccat tcggggctcg
     4921 ttagcgccaa cgacacaacc gcgatcacca agcagcgcaa acagaccgtc agcccccctc
     4981 gaacgcggcc cagccccgcc agcgaccggc gcgccagcac caccaccagc ggcagcagcg
     5041 ccagcaccgc caggtagccc ggctgcatca ggtggatcga ggacagccca tccgcggtct
     5101 gcataccaga cattacgcaa aacggagcgg ttgttgcaac cggcggccgg ggcaaaagtc
     5161 actgaccggc cgggcagccg gacggggggt gctaagccac tggagaaaac gacttccatc
     5221 ggttaatact ggcgccgtga aaaatggacc taatccgacc aaaggccgca cttatcgcga
     5281 ggcgggcgta gacatcgacg ccgccgaccg gttcgtggac ctcatcgcgc cggcggtgcg
     5341 cagcacttac ggcccgcgcg tcattgatag tcacggggcg tttgccggac tgttccgcct
     5401 ggattacgac gaacgcctct tcgcccacaa ttaccgcgat ccggtgctgg tggcctgcac
     5461 ggacggcgtc ggcagcaagc tgctggtggc gatccaggcg cgacgcgttg ataccgtcgg
     5521 gatcgacctg gtcgcgatga acgtcaacga cctgctgtgc tgcggggccg aaccgctgct
     5581 gttcctcgac tacgtcgcgg tgaacaaagt ggagccggag gtgctggcgc aggtggtgcg
     5641 cggcattgtc gccggttgcc agcaagccgg ctgcgccctc ctgggcggcg agacggccga
     5701 gatgcccgat ctctaccggc ccgggcactt tgatctggcc ggtttcgccg tcggcgtggt
     5761 cgagcgccgc cgcatgcttg gcccgcgcca cgtgcgcccc ggcgacgagg tgatcggcat
     5821 cgcctccagc ggtctgcact ccaacggcta cgccctggcg cggcgcgtcc tgctggacga
     5881 tgccgggctt gacccgaaca gccgcgtgcg cgggctggat gagccgctgg tcgaggccct
     5941 gctgcgtccg acgcgcatct acgttcgtcc agtgctggat gtcctgcggc actacaaacg
     6001 gcagcgcatg gtggccgcca tggcccacat caccggcagc ggattgcccg gcaacatccc
     6061 gcgcattctc ccgccgaatt gcgacgccgt gttgcgacgc ggctcctggc cggtaccgcc
     6121 gatcttcggg ctgctcgaac atcacggcgt ggaagaggaa gagatgtacc gcgtgttcaa
     6181 catggggatc ggctacgtca tgattgtccg ccagcagtcg gccggcgtcg tgatccgcgt
     6241 cctggagcgc gccggcgagc aggccttcgt cattgggcgg gtacggcgcg ggcggggcca
     6301 ggtggagatt cgctgatgcc tggccatcgc ctgccgcggc tcgcgctgct ggcctccttc
     6361 gccgttctcc cggttctcac gaccggctgt gcggcgacaa gggccttgcc tccgtccgtg
     6421 gcctatcaat ccgagccgtg gaagttcggc cgtcactgcg gcaccaagct gaccacagcg
     6481 cattatgaca tttacacaac gctgaccgac ccgcaacaga ttgagatgat tccggatctg
     6541 gtcgagcggg cctatgagtt ttaccagcag ctcgttccgc cggtccacga gcccgccgaa
     6601 cgcatgcccg tttatcttct cgccacgcgc aaccaatggg ccacagcgac cgcccatctg
     6661 accggggcgc gggccgagat ttttctccgc gtgcgcaacg gaggcttctc cgatggcggc
     6721 gtttcagtga ttcaatatgt ggagccgtcg ataacggggc cgctgctagc tcatgaaggg
     6781 ttccatcagt atctgcactg ttgcgtggga cccgccgtgc cggcctggct gaacgagggg
     6841 ctggccgtgg tttgcgaagg ccagcgctgg acggcgacgc gattgaggga gtttgacccg
     6901 cacttcaatc cgacacgcca aagccggctc gcggaggctg tcaaccgcga ccgcctcttc
     6961 ccgctggaag aactgctgag gacgcacgcc ggcaacgtag tccaggggac ctcaacgcag
     7021 gtgggcacgt attacgctca gctctgggcg ctgatgctgt ttctcaaaga gggcgaggac
     7081 ggcaagtacg cggcgggttt cgaacgtttg cggcgcgagt tggcgacgcc ggccgcggcc
     7141 cagcggttgc gggccgagca aatctgggcc gaagacggcc aggtcaaccc cggcgaggcc
     7201 ctgttccgca gctacatctg cgacgatttg ccgacgttcg agcgcgagta cgtggcgtac
     7261 ctgcagagtc gaatcagcgc ggcttattga agtggcctag acgcggcatg ttgggtgggt
     7321 gccatgtcgg gtgccatgcc caagccgcgt ggcggcgcgg gcatgtgttc gcccgtcggc
     7381 ccaatttccc aggtgtgact gtgggaaagt gggaatgtgg gaatgtgggg aagtgccgtc
     7441 ctggcccttc gcacgtccga tgtggtgcgc ccgcgcgtta agggagggca tgccctcctc
     7501 catcgtgtct ctggcacccg gcacccggct ttctgcccca gttgtgccac cccccttttt
     7561 gtcacccccc tctccccttt ttgtcgcgcg tctttgtcca attacccgct ttgtaacatt
     7621 gccactaaac tctgttaatg ggccatggaa ggcaggatgg agagttgggc aacttacggc
     7681 ggtacaggtg agctatggca gacgtccgca ttgagcgtga ttcgatgggc gagatggagg
     7741 tgccggcgac ggcgcgctat ggggcgtcga cgcatcgggc gctactgaat ttcccgatca
     7801 gcgggtaccg ctttgggcgg cggttcatcc gcgcgctggg gctgatcaag tacgcggccg
     7861 cgatggccaa cgaactgctc ggccggctcg atccggttcg ggcgcgggtc atcaaggagg
     7921 cggcccgcga agtcatggac ggcaagcacg acgatcagtt cgtcctggac gtgtttcaga
     7981 ccggttcggg caccagcacc aacaccaacg ccaacgaagt tatcgctcac cgctccaacg
     8041 aactgcttac gcacggtgaa gcggctggtc cgcagagcga gctccgcgtc catccaaacg
     8101 accacgtgaa catggggcaa tcgtcgaacg acgtgatccc cagcgccatt cacatcgccg
     8161 ccgtcgaagc cctcaaggcc gacctcatgc ccgccctgca ccgcctgcac aacgccctga
     8221 aagccaaggc cgaagagttc gactcgatcg tgaagatcgg gcgcacgcac ctgatggatg
     8281 ccacgcccat tcgcctggga caggagttca gcggctacgc cagccaaatc gagcacgccg
     8341 accgccgcct gatgcagaaa atcgtcacct tgcgcgagct gccaatcggc ggaacggccg
     8401 tcgggaccgg gctcaacacg cacccggact tcgcccgccg cgtctgcgag attatcaacc
     8461 atgaaactcg cgagagtttt caggaggccg ccaaccattt cgaggcgcag cacgccaagg
     8521 atgcagtggt ggcggccagc ggcgcgctga aagtactcgc gatcagtctc acgaagatcg
     8581 ccaacgacat tcgcctgcta ggcagcggac cgcgctgcgg catcggcgag ttgctgctgc
     8641 ctaccatcca gcccggcagc tcgatcatgc ccggcaaagt caacccggtg attcccgagg
     8701 ccgtgcttca ggtctgcgcc caggtggttg gttcggacgc ggccatcacg tacgccgccg
     8761 ggctgctggg caacctcgat ttgcacgttg ggatgccggt catggcacat aacctgctcg
     8821 aatccatccg cctgctggcg aacgcgtgca acgtgtttgt ggacaagtgc gtcagcggca
     8881 tccaggcgaa caggcaacgc tgcgaggagc tgatcgaacg atcgctcgcg atgtgcacca
     8941 gcctggctcc ggtcatcggt tacgacgccg cggcggcgat tgcgaaggaa gcccactcaa
     9001 ccggcaagac cgtgcgacag gtcgcgtccg aaaagggcat tcttgagccc gccaggctgg
     9061 aggagctgct ggatccgcgc tggatgactc atccggcgta ggatgactga tccggcgtaa
     9121 gctccctatg ggctaaccat caacgcggcc cgccgtgtcg cgtgccagca catcgcgcgc
     9181 cggcacaata ctgtctgcca gaatctgacg ctcgcaactt gaagcgacgt gtactacgat
     9241 tccgctcgtg gtgcgagttg acgttcccag ggaagacgcc gtggccctcc agtttcgaat
     9301 cgtaacgacg cgcttgggag aactggcatt cgtgttcgat gagcgcggcc tgcggcgcgt
     9361 gctcttgcca cagcctgccg ggccccgggg tctgcgcgcg gccgtgaaac gcgagtttcc
     9421 cgccgccagg gaagattcgc gtctggcgcc acgcctggcc ggccagttgc agcgctactt
     9481 cgacggcgaa cggatcgaat tcgacgtgca attgaacctg gaggcggaaa gcgcgaccga
     9541 attccgccgc gcggtctggt tagcctgtca ccaaattcca tacggccaaa ccacgaccta
     9601 cggcgatctg gcgcgccgcg tcggtcaccc ccgcgcggcc cgcgcggtcg gaaccgccat
     9661 gcgctccaac ccctgcccga tcatcatccc ctgccatcgc gtgctcggct gcaacgactg
     9721 cctgggcggc tattccgccc cgggcggtat cgaattcaag cggcgtctgc tggagatgga
     9781 aggggcaacg ttccgcaccg ccctcaagga ggaaccggtt tgaacgtgca cacgcgcgat
     9841 gtaacgccgg ctcgatgcgc gccggcgctt acctgcgcga tttgcagtgg catagcctgc
     9901 gcctgggccg gcgagcccag gcgctgccag tctgacggct gacggcaggg aaggaagtgg
     9961 ccagacagtt gcacagattg gttgccccgc agtttacgcc gttgcttccg cagtggcgcg
    10021 atgccggcgt gtatcaactg tggattcggg tgaaccggcc ggtggtgttg cgcgtgggcg
    10081 gcctcgggcg gctgcacttc gcccctggca catacgtata caccggccgc gcggcgcgtg
    10141 ggctgcgccg ccgcgtgctg cgccacgtgc gcggcgcgga gcattttcat tggcacattg
    10201 actaccttct cgccagtccc gcggcgcgcg tcatacgggt taatctggcc gcgaccgatc
    10261 cggcggaaga atgccgcgtc aaccgcgccg ccggttgcgg tgcgctggcg ccggtacagc
    10321 ggttcggcgc gtccgactgc cgcagtcgtt gcccggcgca cctgtggctt atgatgcgcg
    10381 gggcgcgtgc ggcggctatt gatgtcgccc ttagcgggga gaaagagtgc aaatgacggt
    10441 taccacaacc ttaaggacgg ccgcggattc cgaacccgcc cggcggaagg cgcccgggtg
    10501 ggtgatcgcc tgtgtcgcgc tgtacgcggc aggcgtattc ctgccgtttc tcggcgcccg
    10561 cacgctgacg ccgcacgaag tcatggtgtc gcatccggcg ctgcgcattt tggaggacgg
    10621 acactggatt gtgccgcgct tcgccagcgg cttctggctc gacaagccgc cgttggtcaa
    10681 ctggctgacg gccgcctgtt tcgccatcgt cggcggattc agcgagttcg ccgcacgcct
    10741 gccggctgcg ctcagcgccg tcggactgtg cgttctcatg gccgtcctgg ccggccgttt
    10801 cttcagcacg agggttgccc tgctgactgg gctggtgcag gcaagctgtg tctatatgta
    10861 tatgcaaggg cggctgggcg agatcgacat tgtgctcgcc ttgctgctga cggcggccca
    10921 tggtgttctt ctgtggcatt ggggacgagg cggattcgat ctgccttggc gcgccagcct
    10981 gcttttctac ttcctggcgg ccttgggggt actggccaag ggaccggtgg cggtcgcgct
    11041 gctcggggca actgtgatag cgtacgccat ctggcggcgg tcctggcgcc cgatccgggc
    11101 cgttctcatc tcgccgggcg tgctggtgtt cgtgctggtg gccggcggct ggcacctggc
    11161 ggcggcatac tcggtaggcg cagaagccct gcaggaatgg aactacaaca ctgtcgcccg
    11221 ctttctcggt ttgcaccgcc tgggttcgca atcgttcttc ctctacttcc gcgacattcc
    11281 atggatggtt ttgccttgga cggttgcgct ggtgctgggg gcgcgcgtcc tggcggccgg
    11341 ctatcgcggc cccgatggcc ctgtgcaccg cttcctctgg tcttggtttt tgggaggctt
    11401 ggggtttctg ctgctggcgc tgttcaagca caagcactat gcgattccga ttctgccgcc
    11461 gctgtcgatt ctcgcggccc tggtgatcga tgctcactta gtcaagaaag gcgtgcacgc
    11521 ccgccgcttc tatacgatcg tcttcgccgt ctgcctgctc ggtttcgcct tcgtgggcgg
    11581 ctacgtcatg ccccggcagg accaccggcg agtgacggcc gaattcgtgg ccggcgcgac
    11641 tgccggcgtt ccagcggacg agacgctata cgtcattggt ctggcacagt catccgccta
    11701 tccgtacatc gcacacaaac gctgtgcgta tctggatcgt ttggacgaca tccgcgatga
    11761 gcttcgcgag cgccagcgtc ccatgtgggt gctgagtctg cggaagtacg cgcagttggc
    11821 cgccgaccgc ggcctcagct tcgagcaagc agccgccgag cagccgcgcc gcaaacatcc
    11881 ggagccggaa actctggttc tggggcgatt gtgggaatcg gaaccggctg cctcgtcgcc
    11941 cgccgggcca cgctgattgc cgggacatcg agagggatct agggatcaag ggatcgaggg
    12001 atcaagagga gagcagagcc ggggcgtgtc gccccggctc tgctaccttt tctttgcttt
    12061 ggcgggcttg gcgtcttggc gctttctcag cgtcttggcg gtttctcggc gtcttggcgg
    12121 ttgccttgtg caaagcggag ttttgcactc cgaacagcgc gttgcgaagc ttagcgcgtg
    12181 ggctggcggg tggactggcg ggtgggctgg ctggtggact ggcgggtggg ctggcggcgg
    12241 ggagggaagg cgcattaaat tcaatgtatc tgactcggcg gcggtcacgc ggcgtgtgta
    12301 cgctgcgcgc cggccgagca ttcgggagcc tgcaatgact gcatcctgga cagccgaaag
    12361 cattctcaac ctggcccgcg ggtatcagga ggcgtgcata ctggcagccg ccgcggaatt
    12421 tgacttgttc acgcgcctgg cggacggcgg ccaaacggcc gaggagttgg cggccgcggt
    12481 gcaggctgac gtgcgggcga cgcgctacgt tctcgacgcg ctgacggccc ttgaactgct
    12541 cgagaaaacg gggcagcgct atcgcgtgcc cgagcagctt gcaccgttgg tgacgagcgg
    12601 cggcccgggc agcgtgttgc ccatgctgca acaccaggcc gcgtgcgtgc ggcgctgggc
    12661 gcaactgtcg tgggcgatga agaccggccg gcccgtcgag gccgagacca ttcgcggcgc
    12721 ggaggctgac cgcgaggcgt ttctcgaggc gatgaattgc gtttcaatgc cgctgttgtc
    12781 gggcctcatt aaggcgctgc agccgttgcg cttcagccac ttgctggacg tgggtggggc
    12841 cagcggcacg tggacgatcg cgttcttgcg ggccgcgccg gaggcgacgg ccaccatctt
    12901 cgatttgccc gacgcgctgc cgctggccaa gcggcgtctg gcggccgagg gcctggctaa
    12961 ccggatacac ctcgtgggcg gtgattttta tgaagaccca ctgccgcagg gagcggacct
    13021 ggcctggctg ggagcgatca tccatcagaa ctcgcgcgag cagaatcgcg cgctgctggc
    13081 caaggtttat gaggcgctgg cgccaggcgg ccaggtcgtg attcgcgatg tcattatgga
    13141 cccgtcgcgc acccgccccg taatgggcgc gctgtttgcg ataatgatgc tcctggcgac
    13201 cgacgccggc ggaacgtaca ccttcgagga agtatctgag gaccttagcg cgaccggatt
    13261 catcgacgct accctggtac gccgcggcga gttcatggat tcgctgatcc gcgctaccaa
    13321 acccggccag ttgccggcgg attaaccgca gtaatgcacc tgccgccctg cgccgataca
    13381 caacgcgatg tatcggcgcg ggattgctgg cctgttgtgc atgtcagctc tggcgctgct
    13441 ggcgcctgtc tttgcttgtc tcggttcgga ttgggagccg gatgcgtggg ccggtgacgc
    13501 ggaaaaaggc atggtctaca tgctgcccgg catcgatggt cagcccggct cgttgctgga
    13561 ggcttacgcc gggctgcgcg acggcggtgt gcaagcagaa atccgcgttt tcaattgggg
    13621 gccgtacgcg atgctcaacc tcgcggatta cgtgcgcaac cgtgccgtgg cccgtgccat
    13681 cgcggatgag atagtcgcgt tccaggacca gtacccggag gcgccggtcg acctggtggg
    13741 gtattccggc ggcggcgcgc tggtgttgct ggtgctcgaa tctctgccgg ccgagcgcag
    13801 cgtgcgcaaa gctgtgatcg tgcaggcggg cgtcagtccg gattacgatc tcagcgaggc
    13861 gctgtggcac gtcgaagacc agctcgtgag cttctactgc ccgtccgact ggctggtgct
    13921 gggggtcctc acggagctgg tgggcacggt cgaccgggcc cacaccgcgt cggccggcaa
    13981 ggacggtttc gacacgcagc gcgctattcc cgacccctcg ctggccgaca agctcgcgca
    14041 aatctg
//
LOCUS       DAOSYS010000073        13978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3686_length_13978_cov_5.590893, whole genome shotgun sequence.
ACCESSION   DAOSYS010000073 DAOSYS010000000
VERSION     DAOSYS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3686_length_13978_cov_5.590893"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLU99_06240"
     CDS             complement(<1..639)
                     /locus_tag="PLU99_06240"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOO16695.1"
                     /translation="MKYLEFETPASKQAGELASDYKLKNHGLDYLDRVFWNLPVPALV
                     EEAIFRGEGHMVAGGPFIVNTGKWSARAAADKYIVKEPENENRIWWGEYNRPASLGTF
                     NSILVRLQAFLQGEELFVQDCFVGADPAYRMAIRVITEKAWHSHFARNMFITTDNRDE
                     LKRHVPEFTIIALPSFKVDPRIDGTRTETAIMLNFAEKLGIIANTGYGGEIKK"
     gene            complement(774..1988)
                     /locus_tag="PLU99_06245"
     CDS             complement(774..1988)
                     /locus_tag="PLU99_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO16696.1"
                     /translation="MSISQIGRTIGESVTLKLNEKAAILRAKGDPVIHLGGGEPKSKP
                     PMDALVAAAGLLNSGEVRYTPADGIPALKQAIIRYTEEFYNRVVEPENVMASGGAKQA
                     IMCAFQAILNPQEEVVYPVPYWVSYPEMAKLCGARGVPVMPEDGSFYPRIQDIERACG
                     SYTKAVIINSPNNPSGAMYSEQFIADIVEFCEKRDLYLIMDDIYHRLIFDGRKPINCW
                     NYVKDKSENSKLIIINGVSKQYAMTGFRIGWAVANKKLIEVMRNIQSHQTSGPSVLLQ
                     AAAAAALNGIQSSIESLRTTLENNRNVLIDRLKSFDGVKVNKPDGTFYSFADFSAYNK
                     DSTQLAEFLLNKVQVVVVPGVEFGAEGYLRISYCGTIKDITEGIERMKWALDPNAPNE
                     LYIGMRKLVRDW"
     gene            2465..3529
                     /locus_tag="PLU99_06250"
     CDS             2465..3529
                     /locus_tag="PLU99_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOO16697.1"
                     /translation="MTARINTAKRAQFSPGELAVVLSHYDVGVIASARDFPRGSRHSP
                     KLLLCAADGRQFLLKKRAAGQDDPFRVAFTHVLLTYLRERRFPVPALIGTRDDQNSLL
                     QLNGATYEMFEYIVGERYNGSLDQTQQAGELLRQFHDEVREFQTEWASPAGAFHDCPT
                     VRQALNAIPATTAGHDSVIGHEAELLALVQDLHARYDAAAERVARCGADGWARSIIHG
                     DWHPGNLLFREGRVAAVLDLDSARLQPPILDVANGLLQFSILRTRAGPEEWPSAFDEA
                     RMQRFWAGCGLRANGSSAQRSAITDLMIESLIAEAVLPIAETGSFGHLTGFGVLKMVR
                     RKAEWLAEHRERLTRQFEAK"
     gene            complement(3653..4060)
                     /locus_tag="PLU99_06255"
     CDS             complement(3653..4060)
                     /locus_tag="PLU99_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16698.1"
                     /translation="MPQLNATRSRTHPRVRFATQEAVFDLLLAAQALLTEPTSGHHGH
                     RQMALFKHGPMTLALYLFESGSKLPNHVVDGPIILHVLDGEVAIRTSSSDYQLSAGQL
                     LRLAPRVEHDISALQESRLLLTFCLEGPDSHRI"
     gene            4547..4945
                     /locus_tag="PLU99_06260"
     CDS             4547..4945
                     /locus_tag="PLU99_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO16699.1"
                     /translation="MRLGRASSYAVFAVVYLSEHGDGTPLQGREIAESCGMPTGRLLK
                     ILQQLVRSRILASERGPSGGFRLRRPAEDISLLEIIEAIDGPIEGDVAVRQQVSGMEN
                     ARTAVEKACMQVAEFARKQLKGVTVKDLIS"
     gene            complement(5473..6882)
                     /locus_tag="PLU99_06265"
     CDS             complement(5473..6882)
                     /locus_tag="PLU99_06265"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOO16700.1"
                     /translation="MLKRHSQLILALLIVADAAAVAVGWLLSYWLRFKYLPVSPVKGV
                     PGLTDKFLPMLGPVVLAHLIVFARVHLYRPRRSDRPFRETRDIIKAFCVAIVIVIVID
                     YVMPQTYKISRGFVATYAIVGTACFALFRGTLRLTLQALRRRGYNQRSAAIIGSGRNA
                     QRLYLALEKHNWTGLRVVYFVDERPAERAGTLRGVPVRGPLSELARIVEQHPVDSIFI
                     ALQADQADKVDELVRQLGTSMADIRIVPEFNPYLAMRPAVSNLDGIPILSLRESPLYG
                     SNALLKRAFDLVIGAICLLIAAVPMGIIALAVKLTSPGPVLYKQRRMGLDGREFTMLK
                     FRTMRADAEAETGPVWADRDDARRTPLGSFLRRTSLDELPQLFNVLIGDMSLVGPRPE
                     RPEFISQFRQQIPRYMLRHKMKAGMTGYAQVKGLRGNTSLKKRIQHDVHYINNWSLGL
                     DLKILAQTVGGVWFSRHET"
     gene            complement(6961..7317)
                     /locus_tag="PLU99_06270"
     CDS             complement(6961..7317)
                     /locus_tag="PLU99_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO16701.1"
                     /translation="MFRITFIDPDKHEHVVAVETPGTPHSGYGLPGSLLDIALAHGVD
                     IDHACGGACACSTCHVIIRQGYGACKPAEEDEEDQLDQAYGLTTRSRLACQCVPDGSA
                     DLVVEIPRWNRNLVRE"
     gene            complement(7349..9319)
                     /locus_tag="PLU99_06275"
     CDS             complement(7349..9319)
                     /locus_tag="PLU99_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO16702.1"
                     /translation="MIVGIDLGTTFSLVAYADESGPHILRDRNGEARLPSVITAAPAA
                     ADGSAEARFVIGWEARDHAVENPRSTVYSIKRLIGQGAQDIAGEAQYLAYAVRPGPRE
                     TVQVELNGRLFSPEEISALILRELRERAERQLGRPVRKAVITVPAYFDDAQRQATRDA
                     GQAAGLEVMRIVNEPTAAALAYGFGVRGEPARAAPEVKPHAQTLHRVSLPLPRCSDAV
                     TLDTPAATGQTVAVYDLGGGTFDISILRIEEGVFQVLSTAGDTHLGGDDFDRTIVLLV
                     QREVREQFGVSIDSPATRQALRTLAESVKVRLSEQATAAIELDLGQQRVYRRTITRDE
                     FEQLTVDLVNRSLALCDRALADAGLKAGDLERVILVGGSTRMPLVRRRVSEHFGKDPY
                     TALNPDEVVALGAAIQAAILAGAHPDMLLLDVTPLSLGIETMGGAVSKLILRNTTIPC
                     RATEMFTTFVDGQTAIKLNVLQGERELAADCRSLGDFELRDLPPMPAGIPKIEVEFLL
                     DANGILNVSAKEQRSGKKASIQIIPNRGLTRAEVERMTRESIAHAQEDIDAHRRIDLL
                     NQVEFDIAKTQQMLARVGDQLKETERVAIEKQIADLRQLANETKDLDELHRALTAFGH
                     STLRLAEIGIREALVDRSPGGAVRGSSDTNAG"
     assembly_gap    9327..9426
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9758..10207)
                     /locus_tag="PLU99_06280"
     CDS             complement(9758..10207)
                     /locus_tag="PLU99_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOO16703.1"
                     /translation="MENPSQPNVEPTPAQQPTASFAGLRQRAVPATPTEGIRLSPKAA
                     EQIKNILQKENYPDTMYLYVGVKGGGCSGLQYVLDLRDGAHVPASESDEAFVSEDILI
                     VCDLKSYIVGNLSGTEIDYRDGLMGAGFTFNNPNAKHTCGCGSSYSA"
     gene            complement(10253..10669)
                     /gene="iscU"
                     /locus_tag="PLU99_06285"
     CDS             complement(10253..10669)
                     /gene="iscU"
                     /locus_tag="PLU99_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOO16704.1"
                     /translation="MPYGDKVIDHFQHPRNVGSLDKTSPNVGTGIVGAPECGDVMKLQ
                     ILVDENDNIVDAKFKTFGCGSAIASSSLATEWLKGRKLDQALDIRNTDIVRELALPPV
                     KIHCSVLAEDAIRAAVEDLRKKKGARRSAAEGNGAK"
     gene            complement(10684..11895)
                     /locus_tag="PLU99_06290"
     CDS             complement(10684..11895)
                     /locus_tag="PLU99_06290"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOO16705.1"
                     /translation="MRRIYLDNNATTQVDPRVLEAMLPFFCDKFGNAASRNHAFGWEA
                     EQAVEAAREQIASLIGADAKEIVFTSGATEGNNIAVKGVAAMYAERGKHIITQATEHK
                     AVLDPCHWLAEQGYRVTVLPVDAKGMVDLEQLKQAMTPDTILVSIMHGNNEIGTVQPI
                     AEIGRLCKEREVLFHTDCCQTFGKLPINVEEMGIDLLTCSAHKIHGPKGVGALYVRRK
                     RPRVRCAPVLHGGGHERGVRSGTLNVPGIVGLAKAAEICGREMESEPARLAALRDRLK
                     DAIISKLDAVRLNGHPTLRTPTNLNLSFAYVEGESLMMGMPEVAVSSGSACTSASLEP
                     SYVLRAIGVGDDLAHGSIRFSLGRFNTADEVDYVAERVVATVRKLREMSPLYEMAQEG
                     VDLKAVEWTRE"
     gene            complement(11892..12356)
                     /locus_tag="PLU99_06295"
     CDS             complement(11892..12356)
                     /locus_tag="PLU99_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO16706.1"
                     /translation="MLSLTKKSEYALIAICHLARVGERVVSAREIAELHQVPLPLLMN
                     ILKQLNQAGCVNSVRGARGGYSLAVPAETLTLSAVLRAVEGPVRLVNCAPVGTGSRVC
                     ERAGICSIRSPIHKLHHQFLRFLDEVTVSDVAFDEAYYERPVGMNGEAAVSE"
     gene            complement(12500..12811)
                     /locus_tag="PLU99_06300"
     CDS             complement(12500..12811)
                     /locus_tag="PLU99_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOO16707.1"
                     /translation="MAGEASQLAITARPGGIELRVKVVPGASRSRLAGRLGDALKVAV
                     AAPPEGGKANAALIELLASALGVKRADLAVVSGHGNPLKRVSVKGISVADARARLSGA
                     E"
     gene            complement(12801..13547)
                     /gene="lptB"
                     /locus_tag="PLU99_06305"
     CDS             complement(12801..13547)
                     /gene="lptB"
                     /locus_tag="PLU99_06305"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOO16708.1"
                     /translation="MLLEARKLVKAYNGRTVVDHVTYRVDEREIVGLLGANGAGKTTS
                     FRMTVGMIAADEGQVIFDHADISGLPMYQRARRGMGYLSQEPSVFQRLTVEDNLLAIC
                     ETLRLTRKERERKVAELLDQFGLTIVRKNLAKNVSGGERRKLEIARALITQPRLILLD
                     EPFSGVDPKAVEELRNEILKLRDRGIAILLTDHNVHETLRVTDRSYVIHEGRVISDGT
                     PQKLINDPLVRDHYLGNTFKGDEFGPANGG"
     gene            complement(13775..>13978)
                     /locus_tag="PLU99_06310"
     CDS             complement(13775..>13978)
                     /locus_tag="PLU99_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16709.1"
                     /translation="RRRSCIFPACCPKAGPGDGVCLTWDASDLCGNGSIWRLSKIHHS
                     FGVFNRLARRLERNRGNAIESPN"
BASE COUNT         2465 a         4263 c         4416 g         2734 t
ORIGIN      
        1 cttcttgatt tcgccgccgt agccggtgtt ggcgatgatg cccagcttct cggcgaagtt
       61 gagcatgatg gcggtttcgg tgcgcgtgcc gtcgatgcgc ggatcaacct tgaaggatgg
      121 cagcgcgatg atcgtgaatt cgggcacgtg ccgcttgagc tcgtcgcggt tgtcggtggt
      181 gatgaacatg ttccgcgcaa agtggctgtg ccaggccttt tcggtgatga cgcggatagc
      241 catgcggtaa gccgggtcgg cgccgacgaa gcagtcctgc acgaacagtt cctcgccctg
      301 caggaatgcc tgcagccgca cgagaatact gttgaacgtg ccgaggctgg cgggccggtt
      361 atactcgccc caccagatgc ggttttcgtt ttccggctct ttgacgatgt acttgtcggc
      421 cgcggcacgg gcgctccatt tgcccgtgtt cacgatgaac gggccgccag ccaccatgtg
      481 gccttcgccg cggaagatgg cctcttccac cagggccggc acgggcaggt tccagaacac
      541 gcggtcgaga tagtccaggc cgtggttctt aagcttatag tcgctggcca gctcgccggc
      601 ctgcttggat gcgggtgttt cgaactcgag atacttcatc gcttgctccg caaaagccgc
      661 tccgggcggc tgttagcttt cgcttaggac ccggggtgtt ttgaggtgcc gatctgccac
      721 ctcaaactga cccggctcta aggtcgcaaa ttgcgacctc aaaaccggtg ccgttaccaa
      781 tctctcacca acttccgcat tccgatgtag agctcgttgg gcgcgttcgg atccagcgcc
      841 cacttcatac gctcgatgcc ttcggtgatg tccttgatcg tgccgcaata gctgatgcgc
      901 aggtaaccct cggcgccgaa ttcgacgccc ggtaccacca ccacttgcac cttgttcagc
      961 aggaactcgg ccagttgcgt cgagtccttg ttgtaggcgc tgaagtccgc aaagctgtag
     1021 aacgtcccgt ccggcttgtt caccttcacc ccgtcgaaag acttcagccg gtcgatcagc
     1081 acgttgcgat tgttttccag cgtcgtgcgc aggctctcga tgctcgattg aatgccgttc
     1141 agcgccgccg cggccgccgc ttgcagcagc accgacgggc ctgaggtctg gtggctctga
     1201 atgttccgca tgacctcgat cagcttcttg ttggcgaccg cccagccgat gcggaagccc
     1261 gtcatggcgt actgcttgga cacgccgttg atgatgatca gcttcgagtt ctcgctcttg
     1321 tccttgacgt agttccagca gttgatgggc ttgcggccgt cgaagatcag gcggtgatag
     1381 atatcgtcca tgatcaggta caggtcgcgc ttctcgcaga actcgacgat atcggcgatg
     1441 aactgctccg agtacatcgc gcccgagggg ttattcggac tgttgatgat gacggccttc
     1501 gtgtatgacc cgcacgcccg ctcgatgtcc tggatgcgcg ggtagaagct gccgtcctcg
     1561 ggcatcaccg gtacgcctcg ggcgccgcag agcttggcca tctccgggta gctcacccag
     1621 tacgggaccg ggtagacgac ctcctcctgc ggattcagga tcgcctggaa ggcgcacatg
     1681 atcgcctgct tcgccccgcc cgaggccatc acgttttcgg gttccaccac ccggttatag
     1741 aactcttccg tgtagcggat gatcgcctgc ttcaacgccg ggatgccgtc cgcgggcgtg
     1801 tagcggactt cgcccgagtt caacaagccg gccgccgcca ccaacgcgtc catgggcggc
     1861 ttgctcttag gttcgccgcc gcccaggtga atcaccgggt cgcccttggc gcgcagaatc
     1921 gcggccttct cattaagctt gagagtgacc gactcgccga tagtcctgcc gatctggcta
     1981 atgctcatcg aaaattcctt gaaccttcag ccttcagccc ctgcggccga atcaaaaaaa
     2041 gaaacggccg acgctatcgc ctgggcgggg gactgtcaat tccgtccctc gctggctgga
     2101 acctttcgtt gcaagcaaga atcgttcccg actccgcgct tgcgtctccg cgggaaggca
     2161 tgcccgcgcc tggcgcttgg gcatggcacc caaggttaac cccggctccg caccaccttc
     2221 atccggttcc aggaacggct tcgctccagc ttcactccct aagcttcaat ttggagcttc
     2281 agcccagctt tgcctgccgc ggtggaaccg cttggcacga tggcgagaag gttcggtttg
     2341 cttggacggg tggtcgcctt aagatgctcc gcattatcgg cgcgagcctg ccggtcgcag
     2401 gttaaatccc gccgtacgtg agctaatgac tgcccggcgc gacgccgctg ccgtaggtta
     2461 ggtaatgacc gccaggataa acactgccaa gcgcgcgcaa tttagccccg gcgaattggc
     2521 tgttgtgctc agccactacg acgtgggggt tattgccagc gcgcgcgatt tcccgcgcgg
     2581 ttcgcggcac tcgccgaagc tgttgctatg cgcggctgac gggcggcagt tcctgctcaa
     2641 gaagcgagcg gccgggcagg atgacccgtt ccgagtggcg ttcacgcatg tcctgctgac
     2701 gtacttgcgg gagcggcggt tcccggtgcc ggctttgatc ggtacgcgcg acgaccagaa
     2761 ttcgctgctg cagctcaatg gcgcgactta cgagatgttc gaatacatcg tcggcgagcg
     2821 ctacaacggg tcgctcgatc agactcagca ggccggtgaa ctactgcggc aatttcacga
     2881 cgaggtgcgg gagtttcaga cggagtgggc ttcgccggcc ggggcgttcc acgattgccc
     2941 cacggtacgc caggcgctga acgccattcc cgcgactacc gccggacatg actcggtcat
     3001 cggacatgag gctgagttgc tggcgctcgt ccaggatctg cacgcgcgct atgacgcggc
     3061 ggcggaacgc gtcgcacgct gcggggcgga cggctgggcc cgaagcataa ttcacggcga
     3121 ttggcatccc ggcaatctgc ttttccggga agggcgcgtg gcggcggtgt tggacctcga
     3181 ctcggcccgt ttgcagccgc cgattctgga cgttgccaac ggcctgctcc agttctcgat
     3241 cctgcggacg cgggccggcc cggaggagtg gccgagcgcg tttgatgaag cacggatgca
     3301 gcggttctgg gcgggctgcg gcctgcgcgc caacggatcg tcggcccaac gttcagcaat
     3361 cacggatttg atgatcgaat ctctaatagc tgaagcagtg cttccgatcg ctgaaacagg
     3421 gtccttcggt catctgaccg ggttcggcgt gctaaagatg gtgcgccgga aggcggaatg
     3481 gctggcggag caccgagagc gtctgacgcg gcaattcgag gcgaaatagt cgagccatag
     3541 caatagcgag gcgcggcaat ccgcccaatg atccaaaccg tcgttacccc gcaaactgtg
     3601 cctcgcaggt catcggacgc ttccgcggcg acacgccccg actctcctac cttcatatgc
     3661 ggtgagaatc cggtccttca aggcaaaagg tcagcagcag ccggctttct tgaagcgcgg
     3721 aaatgtcgtg ctcgacgcgc ggcgccaggc gcagcaactg gcctgcggat agctgataat
     3781 ctgaggaact ggtgcggatt gccacttctc catcgagcac gtgcagaatt ataggaccat
     3841 ccactacgtg attcggcaac ttcgagccgc tttcgaagag atacagcgcc aaggtcatcg
     3901 gcccatgttt gaacagggcc atttgccggt gcccgtgatg acctgatgtg ggctcggtca
     3961 gcagcgcttg agccgcaagt agtaggtcaa acaccgcctc ttgcgttgcg aagcggacgc
     4021 ggggatgcgt gcgactgcgt gttgcattca gttgtggcat gtgcgcctcg tattccgccg
     4081 ggtattgtcg ttttgcacgc ttaacaaacc gccacaaaat tataggacgc acaggccggc
     4141 gaaccatgcg acatgccgga tgaaaatcaa aagaacgcag ccgggaatgc agccccgttg
     4201 ccctcgttga cagagcagag gcaggcgggt agccccggag ttctcgtcaa gtgttccaaa
     4261 agttagtcgc ccgggatccg tgacggcttc cgcggcctac aaaaaccccg caaatcacgc
     4321 caatgacgat ttgccttgac gggcggacat attgcggcga agataagtcg attgaatggt
     4381 tttttcgggc cggtacgcct gggcgggcga cgcgatctgt gcgacacgac cgaagcgctc
     4441 ctcgcggctc gcaattagga tggtgtgagg ccgaattgcg cgggtcgagt tgatgtcaag
     4501 cccggctggc gaagggtgag cgcccgcggg tggaggatgt gagctaatgc gtttaggaag
     4561 agcctcatcg tacgccgttt ttgcagtcgt ctatctctcg gagcatggcg acggtactcc
     4621 ccttcaggga cgggaaatag ccgaatcttg cggcatgccg accgggcggc tgctgaagat
     4681 tctgcagcag ttggtgcgtt cgcgcattct cgccagcgag cgcggtccgt ccggcggatt
     4741 ccggctgcgc aggccggcgg aggacatcag cttgctcgag atcatcgaag cgatcgatgg
     4801 gccgattgag ggggacgttg cggttcggca gcaggtttcg ggcatggaga acgcgcgcac
     4861 cgcagtcgag aaggcttgca tgcaggtggc cgaattcgca cgcaagcagc ttaagggggt
     4921 gacggtgaaa gacctgataa gctaaagaaa ccgacaggca actcaccaca acgagtcgcc
     4981 cttagcgaga caagtagcga ggccgcaagg cgcgccccaa atgccatgcg cgagcgctat
     5041 gcgctcgcgc atacctaccg gaccgcgccg gcacatccgc gcggtagtcg cgctgcgcca
     5101 ttagtcccgg gcaagttata cgggcagacg tgccgccatc aggcggcgcc aaaaaatttt
     5161 tcgacaaaac gacggtccat atcttgttgt atagaaacga cttacggctt tcagagtaca
     5221 gtcggcgaaa aattctcgcc catttctttg taagaatcgc gcgcagttta cgttttatag
     5281 gttggaacgc gatggtcgcc actctcccgg ccacaagcgg cgactgtcaa tagcgcgacc
     5341 gggccgtccc ccggggggaa cggctcggcg cgcttttctt atgcgcctca tcccgaatcg
     5401 tgcccacttg ctgcggctca ggttctgctg gtgccgctcg cggccgctcg ggctttgatt
     5461 cgcgatctga attcaggttt cgtggcggct gaaccagacg ccgccgaccg tttgggccag
     5521 aatctttaag tccaggccca ggctccagtt attgatgtaa tgcacgtcat gctggatgcg
     5581 cttcttcaat gacgtgttgc cgcgcagacc cttcacctgg gcgtagccgg tcatacccgc
     5641 cttcatcttg tgccgcagca tgtagcgcgg gatctgctgc ctgaattggc tgatgaactc
     5701 gggccgctcc ggtcgcggac cgaccagcga catgtcgccg atcaggacat taaacaattg
     5761 gggcagctcg tccagactcg tacgccgcag aaagctgccc aaaggcgtgc gccgggcgtc
     5821 gtcgcggtcc gcccagaccg ggccggtttc cgcctctgcg tccgcccgca tggtgcggaa
     5881 cttcagcatc gtgaactcgc gcccgtccag gcccatccgc cgctgcttgt acagcaccgg
     5941 gccagggctg gtcagtttga cggccagcgc gatgattccc atcgggacgg ccgcgatcag
     6001 caggcagatg gcgccgataa cgaggtcgaa ggcgcgctta agaagggcgt tcgagccata
     6061 tagcggcgac tcgcgcagcg acagaatcgg aatgccgtcc aggttgctga cggccggacg
     6121 catcgcgagg taggggttga actccggcac aattcgaatg tccgccatcg acgtgccgag
     6181 ctggcgtaca agctcatcga ccttgtcagc ctggtcggcc tggagcgcga tgaagatcga
     6241 atcgaccgga tgctgctcga cgatgcgcgc cagttccgaa agcgggccgc gcaccggaac
     6301 gccgcgcagc gtccctgccc gctcggccgg ccgctcgtca acgaaataca ccacccgcag
     6361 cccggtccaa ttgtgctttt ccagcgccag atagagccgc tgtgcgttgc ggccactgcc
     6421 gatgatggcg gccgagcgct ggttgtaacc gcggcggcgc agggcctgca gcgtcaggcg
     6481 cagcgttccg cggaagagtg cgaagcaggc cgttcccacg atcgcgtagg tggccacgaa
     6541 gccgcgcgaa attttgtagg tctgcggcat gacgtagtcg atgacgatga cgatcacgat
     6601 cgcgacgcaa aaggccttga tgatgtcgcg cgtttcgcgg aaggggcgat cgctgcgccg
     6661 cgggcggtac aggtgtacgc gtgcgaagac gatcaggtgg gccagaacga cgggccccag
     6721 catcggcagg aacttgtccg tcaggccggg aacgcctttg accggactga cgggcaggta
     6781 cttaaagcgc agccaataac tgagcagcca gccgacggcg accgccgcgg catccgctac
     6841 gatcaacagc gcgagtataa gttgactatg ccgcttcagc acgaccgcac cgttactaaa
     6901 ccgcgcacat tatccgcagt cgggcgggcg aggatcaagg gaccgggtta atcaatggcg
     6961 ctattcgcgc accaggttgc ggttccagcg tgggatctcg acgaccaggt cggcgctgcc
     7021 gtccggtaca cattggcagg ccaggcgcga acgtgtggtc aggccgtagg cttggtcgag
     7081 ctgatcttcc tcgtcctctt cggccggttt gcaggccccg tagccttggc gaatgatgac
     7141 gtggcacgtc gagcaggcgc aggcgccgcc gcaggcgtga tcgatgtcta ctccatgggc
     7201 cagagcaata tcgagcaggc tgcccggcag gccgtagccg ctgtgcggtg tgccgggggt
     7261 ttctaccgcg accacgtgtt cgtgcttgtc tgggtcgatg aatgtgatgc ggaacacgaa
     7321 tgcaccttca ttcggaagtc gctacttttc agccggcgtt cgtgtcgctc gaaccgcgga
     7381 ccgcgccgcc gggactccgg tcgaccaacg cctcgcgaat accgatttcg gccagccgca
     7441 gtgtactgtg gccgaaggcg gtcaacgcgc ggtgcagttc gtcgaggtcc ttagtctcat
     7501 tggcgagttg ccgcaggtcg gcgatttgct tttcgatcgc gacgcgctcg gtctctttga
     7561 gctgatcgcc gacgcgggcc agcatctgct gagtcttggc gatgtcgaac tcgacctggt
     7621 tgagcaggtc gatgcggcgg tgggcgtcga tgtcttcctg ggcgtgggcg atgctctcac
     7681 gcgtcatgcg ttcgacttca gctcgcgtca ggccgcggtt cgggatgatt tgaatgctgg
     7741 ctttcttgcc gctgcgctgc tccttggccg agacgttcag aatgccgttg gcgtccagca
     7801 ggaattcgac ctcgattttc gggatgccgg ccggcatggg cggcaggtcg cgcagctcaa
     7861 aatcgcccag gctgcggcag tcggccgcca gttcgcgttc gccctgtaag acattcagct
     7921 tgatggcggt ttgtccatcc acaaaagttg taaacatctc ggtggcacgg caagggatgg
     7981 ttgtgttccg caggatcagc ttgctgaccg cgccacccat ggtttcgatg cccagcgaga
     8041 gcggcgtcac gtcgagcagc aacatgtccg gatgggcgcc ggccaggatc gcggcctgga
     8101 tcgccgcacc cagcgcgacg acttcgtccg gattcagcgc ggtgtacggg tctttgccga
     8161 agtgctcgct gacgcgccgg cgcaccagcg gcatccgcgt cgaaccgccg accaggatga
     8221 cgcgctccag gtcgcccgcc ttcagacccg cgtcggccag agcgcgatcg cacagggcaa
     8281 ggctgcgatt gaccaggtcg accgtaagct gctcgaattc gtcgcgcgta atcgtgcgcc
     8341 gatacacgcg ctgttgaccc aggtccagct cgatcgccgc agtcgcttgc tcgctcaggc
     8401 ggaccttcac ggattcggcc agcgtccgca gggcctggcg tgtcgcgggc gagtcgatgg
     8461 aaacaccgaa ctgctcgcgg acttcgcgct gcaccagaag aacaatggtg cgatcgaaat
     8521 cgtcgccgcc gaggtgcgtg tcgccggcgg tgctcagaac ctggaaaacg ccttcttcaa
     8581 ttcgcaggat cgagatgtcg aaggtcccgc cgccgagatc gtagacggct acggtttggc
     8641 ccgtcgcggc cggcgtgtcg agagttaccg cgtcgctaca gcgtggcagg ggcagggaca
     8701 cgcgatgcag agtttgggcg tgcggcttca cttccggggc agcacgcgcc ggctctcctc
     8761 ggacaccaaa cccataggcc aaagcggccg ccgtgggttc gttgacaatg cgcatgacct
     8821 cgaggccggc ggcttgaccg gcgtcgcgcg tggcttggcg ctgggcgtcg tcaaagtaag
     8881 ccgggacggt gatgaccgcc ttgcgcaccg ggcggccgag ttgccgctca gcgcgctcgc
     8941 gcagctcgcg caggatgagg gccgagattt cctcgggcga aaagagccgg ccgttcagct
     9001 cgacctgcac ggtttcgcgc gggccgggcc gcacggcgta ggcgagatac tgggcctcgc
     9061 cggcgatgtc ctgagccccc tggccgatga ggcgcttaat ggagtagacc gtgctgcgcg
     9121 gattctcgac cgcgtgatcg cgagcctccc agccgatcac gaaacgcgcc tcggccgatc
     9181 catctgcggc cgccggagca gccgtgatga cggacggcaa gcgcgcttcg ccattgcggt
     9241 cgcggagaat gtgcggacca ctctcgtccg cgtaggccac cagcgagaac gtggtgccta
     9301 gatcgattcc gacgataatc tcgccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnntgat cgggatggat ggcacgcgag ctgcgcaaat agcggcgccg caattcggct
     9481 tgatcgatgt cgaacgacgg cgccatctcg aaaatctgga agagattcag accctcggcc
     9541 gggtgcagcg tgtggcagcc gccgcagaat agcgggctgt ccatggggcg attgcaggcc
     9601 gtacatttcg ccggcgtcgc gtgggtctgg ctccaatcgg ccgctgacaa gtcctgagtc
     9661 ctttcgtttc ttgagcagct tgcggcggca ttgtacgccg gcggagcttg tattggcatt
     9721 gcccgcgctc agcgcttggg ccgggtgccc agccgggcta agccgagtag ctggagccgc
     9781 agccgcaggt gtgcttggcg ttggggttgt tgaaggtgaa gccggcgccc atcaggccgt
     9841 cgcgatagtc gatctcggtt ccggacaggt tgcccacgat gtagctcttc aggtcacaga
     9901 cgatgaggat gtcttcggac acgaaggctt cgtccgattc gctggccggc acgtgtgctc
     9961 catcccgcaa atccagcacg tattgcagac cgctgcaccc gccgcctttg acgccgacgt
    10021 acaggtacat ggtgtcgggg tagttctcct tctggagaat attcttgatc tgctcagcgg
    10081 ccttggggga gaggcggatg ccttcagtgg gcgtggccgg cacggcccgc tggcgcaggc
    10141 cggcaaaaga ggccgtcggt tgctgagccg gcgtcggttc gacgttgggc tgcgacgggt
    10201 tttccataat caactccaaa agcggcccgg cggccgccaa acgataccac aactacttag
    10261 cgccgttgcc ctccgccgcg gagcgccgcg cgcccttctt cttgcgcaaa tcctcaaccg
    10321 cagcgcggat cgcatcctcg gcgagaacgc tgcaatgtat tttaaccggg ggcagggcca
    10381 attcccgcac aatatcggtg ttccggatgt cgagcgcctg gtcaagcttg cggcccttca
    10441 gccattcggt cgccagcgaa ctggaggcga tggccgagcc gcagccgaag gtcttgaact
    10501 tggcgtcgac gatgttgtcg ttctcgtcca ccagaatttg cagtttcatt acgtcgccgc
    10561 actcgggggc cccgacgatg ccggtgccga cgttgggact ggtcttgtcc agcgaaccga
    10621 cgtttcgcgg gtgctggaaa tggtcgatga ctttgtcgcc gtaaggcacg gtattgctcc
    10681 cgcctattcc ctagtccact caaccgcctt caaatcaacg ccttcctgcg ccatctcgta
    10741 caacgggctc atttcccgca gcttgcggac ggtggccacg acgcgctccg cgacgtagtc
    10801 cacttcgtcg gccgtgttga agcggcccaa cgaaaaacgg atgctgccgt gggccaggtc
    10861 atcgccgacg ccgatggccc gtaggacata gctcggctcc agcgaggcgc tcgtgcaggc
    10921 cgagccgctg gagaccgcga cctcgggcat gcccatcatc aggctctcac cctcgacata
    10981 agcgaacgag aggttcaagt tggtgggcgt gcggagcgtc gggtggccgt ttagccgcac
    11041 cgcgtccaat ttcgagataa ttgcgtcctt gagccggtcg cgcagggcgg ccaggcgggc
    11101 cggctcgctt tccatctccc gcccgcagat ctcggccgcc ttggccaaac ccacgatgcc
    11161 gggcacgtta agcgtgccgg agcggacgcc gcgctcatgc ccgccgccgt gcaggacggg
    11221 ggcacaacgc acgcgcggcc gtttgcgccg cacgtacagc gccccgacgc ctttgggccc
    11281 gtgtattttg tgcgcgctgc acgtcaaaag gtcgatcccc atttcctcga cgttgatggg
    11341 cagcttgccg aaagtctgac agcagtcagt atgaaacagc acctcgcgct ctttgcacag
    11401 gcggccgatt tcggcgatcg gctgaaccgt gccgatttcg ttgttgccgt gcatgatcga
    11461 gaccaggatg gtgtccgggg tcatcgcctg cttgagctgc tccaagtcca ccatgccctt
    11521 ggcgtcgacc ggcagcaccg tcacgcgata gccctgctcc gccagccagt ggcaggggtc
    11581 gagcaccgct ttgtgctcgg tggcctgcgt gatgatgtgc ttgccacgct cggcgtacat
    11641 cgcggccacg cccttgacag cgatattgtt gccctcggtg gcgccgctgg tgaaaacgat
    11701 ttctttggca tcggcgccga tcaaggaagc gatctgctcg cgcgcggcct cgacggcctg
    11761 ctcggcctcc cagccgaagg cgtggttgcg cgaggcggcg ttgccgaact tatcgcagaa
    11821 aaacggcaac atggcctcca ggacgcgcgg gtcgacctgc gtcgtcgcgt tgttatcgag
    11881 gtaaattcgt ctcattcgct aaccgccgcc tccccgttca tccccaccgg ccgttcgtag
    11941 taggcttcat cgaaagcgac gtccgatacg gtcacttcgt ccaagaaccg caggaactgg
    12001 tgatgaagct tgtgtatggg cgagcggatt gagcagattc cggcgcgctc acacactcgc
    12061 gaacctgtgc caaccggagc gcaattgacc agccgcacgg ggccctcaac cgctcgcagc
    12121 acggccgaga gcgtgagggt ctcggcaggc acggccagcg aatagccgcc gcgcgctccg
    12181 cggacggaat ttacgcaacc ggcctgattt agctgcttaa ggatgttcat cagcaggggt
    12241 agtggaacct ggtgcagctc ggcaatctcg cgtgcgctga ccactcgctc gcccacacga
    12301 gcgaggtggc agattgctat gagagcgtat tcggacttct tggtcagcga cagcataggg
    12361 tggcgctccg ccgggaatat agcatctgag gggtaccatt tcaagcatgt aagccgatta
    12421 atggccgcgg gcagcgtaag tcatgttcgc tgcgtgactt atgactccgg gcgcgctgca
    12481 ggctcgccgg ccgttgcgct tattcggcgc cggaaaggcg agcgcgagcg tccgccacgc
    12541 ttatcccctt caccgaaacg cgcttgagag gattgccgtg accgcttacg accgccaggt
    12601 ccgcacgctt tacgcccagg gctgacgcga gcaattcgat cagggccgcg ttggccttcc
    12661 cgccttcggg tggagcggcc acggctacct tgagcgcatc tcccaatcgc ccggctagcc
    12721 tgctgcgcga ggctccggga acaactttga cgcgcagctc gattcctccc ggacgcgcgg
    12781 tgatggccag ttgcgacgct tcacccgcca ttggcgggcc cgaattcgtc gcccttgaag
    12841 gtattaccta agtaatggtc gcggacgagc gggtcgttga tcagcttctg cggcgtgccg
    12901 tcgctgatca cgcggccttc gtggatgaca tagctgcggt ccgtcacgcg cagcgtttcg
    12961 tggacgttgt gatcggtgag caggatcgcg atgccgcggt cacgcagctt gagaatttcg
    13021 ttgcgcagct cctcgacggc tttggggtct acgccgctga atggctcatc cagcagtatc
    13081 aggcgcggct gggtgatgag ggcgcgggcg atttccagct tgcgccgttc gccgcccgag
    13141 acgtttttgg cgaggttctt ccggacgatg gtcaatccga actggtcgag cagttcggcg
    13201 actttgcgtt cgcgttcctt gcgggtcagc cggagagtct cgcaaatcgc caagaggttg
    13261 tcttcgaccg tcaagcgctg gaaaacgctg ggctcttgcg aaagatagcc cattccgcgg
    13321 cgggcgcgct ggtacatcgg caggccggag atgtcggcat ggtcgaaaat gacctgcccc
    13381 tcatcggcgg cgatcatgcc gaccgtcatg cgaaagctgg tggtctttcc ggcgccgttg
    13441 gcgccgagca ggcccacaat ttcgcgctcg tcgacccggt aggtgacgtg gtcaacaacg
    13501 gtgcgcccgt tgtacgcttt tacgagcttt ctggcctcca acagcatcaa aaagtgcctc
    13561 agcgcagggc cggctcccgt tgtggaaaac ttgttgacag cggcgcaagt gcgggtggaa
    13621 acgagggata tggtccgcgg ctgactgccg ataacgcggc cgccttgcga aatcctaaca
    13681 tttcacgaac gtaatcgcgc acggccttgc cggacctgag gttacggcgt tttaacgcct
    13741 tgttataatg cgtcccgcac actggctttg aatcctaatt cgggctttcg atcgcgttgc
    13801 ctctatttcg ctccagacgc ctcgcgagtc tgttgaaaac cccaaagctg tggtggattt
    13861 tcgacagtcg ccagatgcta ccattaccgc acaaatcgga cgcgtcccag gtcaggcaaa
    13921 cgccatcgcc cggaccggcc ttcgggcaac aagccgggaa gatacacgaa cgccgcct
//
LOCUS       DAOSYS010000074        13879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3721_length_13879_cov_5.208767, whole genome shotgun sequence.
ACCESSION   DAOSYS010000074 DAOSYS010000000
VERSION     DAOSYS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_13879_cov_5.208767"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLU99_06315"
     CDS             <1..372
                     /locus_tag="PLU99_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO16710.1"
                     /translation="PVFAGGATEVAGVVAADPAVAPASAPATESQARLPRVVDLGAGK
                     CKACKDLAPILEELKKEYAGRVEVEYIDVWKNKAAAEPYKIRIIPTQIFYDREGKEVW
                     RHEGFLPKADFIAKFAELGVK"
     gene            372..1616
                     /locus_tag="PLU99_06320"
     CDS             372..1616
                     /locus_tag="PLU99_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO16711.1"
                     /translation="MTVDSAPPPVVSVPAKPRQAWGRTLWRGLKWAVGLAVAAAIAYW
                     MWLSPMPVIAHVVQRGEVVAEVMGTGTLESHISASISPKISGLIKAVLVDQGDRVEAG
                     QVLVQLDDGDLSRQVEVAESALSAAHAAVERRQADRARALAVLDQARFDFDRVANLVK
                     LGTGSTTEFEDARKTLRVSEADLSSSEAALVEAQKQVVTAMKTLELHQTRLADASVRA
                     PFAGLITRRDRDPGDIVVPGSSILALVAMDELWLSAWVDETQMAKLQPGQPARVVFRA
                     APGRSYPGAVARLGREVDRETREFLVDVHVHELPENWAIGQRAEIYIETGRKADAVLL
                     PSRFIGWQDGKAGTFVESGGRAAWRPLRLGLRGQESVEVIEGLSPNERAIVPATQKTS
                     LKPGQRVKVPAPGPHGSAAKQP"
     gene            1613..2827
                     /locus_tag="PLU99_06325"
     CDS             1613..2827
                     /locus_tag="PLU99_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO16712.1"
                     /translation="MNLARKDVWHNRGRFALTTLGLGTLLMIVMGMGGIYRGLTTEAT
                     QLVDKVGVELWVVQRGTRGPFAEISRVPKTLVDRVAAVPGVAEARAFVYHTIQRLRAG
                     KPLRASVLGLDWPTDKGEWLPLSAGRPLAQAHFEMIADRSLGLTLGERLPLGKEIYTV
                     VGLTSGMSDPAGNGFLFLTLADSQAVQFDQSPEGIRLERQARRARAEQIDLGNTQPTM
                     LERAAGPGSQLPALPPNQISAVMTRLAPGVNEAAVKATIDGWADVSVYTRADEAKMLL
                     MGVVERSRRQLGLFRVLLTTVSGIIMALILYTLTMDKLHDIALLKLIGASNWVIVKLI
                     MQQAIALGALGYAVAWLLGQKAYPYFPRRVILTPDDLLMLAGVVLGVSVVASLVGIWK
                     ALRVQPNVALLG"
     gene            2837..3574
                     /locus_tag="PLU99_06330"
     CDS             2837..3574
                     /locus_tag="PLU99_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16713.1"
                     /translation="MRNAAIETHGLTKVYGSGHTEVVAMRDASLCVAHGQCVALIGPS
                     GAGKSTFLTAVGLINPPTAGRILIGGRLVMEGPRALTNPRTFRRKHIGYISQKANLIR
                     FLTARENVQVAMELNGESSRAARARALELLEYLGVRERAAGYPPELSGGEQQRVAVAR
                     ALANRPSVILADEPTAALDGPRGRQVMELFRRVAHEQGAAVIVVTHDHRALDVFDTIY
                     EMEDGVIRSGAAELSAASTFSGGKARP"
     gene            3616..4293
                     /locus_tag="PLU99_06335"
     CDS             3616..4293
                     /locus_tag="PLU99_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOO16714.1"
                     /translation="MLESFAENLAEIIKTNIWLAPLAALVGGLLTAANPCVLAAAPLM
                     VGYVAGQEKRTVARSFLLSLTFAIGLTITFGLMWFSVWSASSLLPASWWKYIAVVVCL
                     LMGLHLLGVLHIRIPAPTGLQPKQKGFIGALLLGLLFGLISIPCAGPILLALSAVTPL
                     YGLAFGILLLSAYSLGHCGLVLVGGTSIGLVQKLADSKGWTRGTDVLRRLAGVLIAGV
                     GLYLLFS"
     gene            complement(4465..4537)
                     /locus_tag="PLU99_06340"
     tRNA            complement(4465..4537)
                     /locus_tag="PLU99_06340"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4502..4504),aa:Ala,seq:ggc)
     gene            complement(4611..4683)
                     /locus_tag="PLU99_06345"
     tRNA            complement(4611..4683)
                     /locus_tag="PLU99_06345"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4648..4650),aa:Phe,seq:gaa)
     gene            complement(4900..5925)
                     /locus_tag="PLU99_06350"
     CDS             complement(4900..5925)
                     /locus_tag="PLU99_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO16715.1"
                     /translation="MNARNVDDSRPIKELPGVSNGIGRRSALKTLAATLGAVVLGPAG
                     CRTAPARNEPVAQPVTAAGSDAGRVEPLFKISLAEWSLHRQLGAGQLDHLDFPEAAKR
                     DYGIEAVEYVSTFFKSAGADYVGELRRRAVDAGVRSLLIMVDGEGALGDPDEAARRRT
                     VENHYKWVEAARLLGGHSIRVNAQSSGSPEEQLALVADGLRRLTEFAAERDLNVLVEN
                     HGGLSSNGAWVVALMKRVDHPRCGTLPDFGNFLPPNAADNAGYDRYAGVAEMMPYAKA
                     VSAKSHDFDESGNEVDIDYRRMIGIVLRAGYHGYIGIEYEGSRLAEADGIRATKRLLE
                     RVRDELS"
     gene            complement(5996..6829)
                     /locus_tag="PLU99_06355"
     CDS             complement(5996..6829)
                     /locus_tag="PLU99_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOO16716.1"
                     /translation="MPDRAFAVISDIHSNLEALRAVLDDIEERGVTEIVCLGDVVGYG
                     PNPKECLDLVRERCRVCLMGNHDHALLYEPCNFNVAAERACFWTRQLLEDEEDKELRN
                     ARWQFLGNMPVRFQDQDILYVHASPRRPINEYIFPEDVFTNPQKVVANFDRLDRRLCF
                     VGHTHQPGVFLDDPYFDPPNELPDSPFYEVEDERAIVNVGSVGQPRDRDPRASYVLVY
                     RQNDVLGTGTESLVAAALSCAIEQIEFVRVEYDIEKTVKKILALPALDELLGTRLYDG
                     R"
     gene            complement(6970..7593)
                     /locus_tag="PLU99_06360"
     CDS             complement(6970..7593)
                     /locus_tag="PLU99_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO16717.1"
                     /translation="MQQTPVQRYYRFHAAIYDWTRWAVLHRRKLAIQRLELRPDSHVL
                     EIGCGTGLNFGAILKQLDPQAGRLTGVDFSRDMLGRARGLIERRGWLNVDLVSADATA
                     LALGERFDAVLFAYSLSMIPDWERALARAWDHLLPGGRLVILDFGRFERWGPAGWLAR
                     AWLRANHVATGRAYPQRLAQVLGPVSYEPWLGGYAFVATARNLRRAN"
     gene            complement(8412..8807)
                     /locus_tag="PLU99_06365"
     CDS             complement(8412..8807)
                     /locus_tag="PLU99_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16718.1"
                     /translation="MRKTSPGFAAIYGQSAVAEGLKLKQVAGAGYRKALEFLVKDYCK
                     ILEPSKADSIEKTALGACINNFVTDENIKTTASRAVWLGNDETHYMRQYDDFDIEHLK
                     ELIDLTVNWIDTDVRTREFQQRLQKRETD"
     gene            complement(9516..10043)
                     /locus_tag="PLU99_06370"
     CDS             complement(9516..10043)
                     /locus_tag="PLU99_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16719.1"
                     /translation="MVTRVVKIWCASLLCTALVALAGCASGEGNSAPSAGPVAQQAAP
                     AVSPQASAVAPHAAPQPTEVAPQDSAVAAQDSAVAPPPSAVTPQPAATPVSAVEATPP
                     APSVAAENPGGPKRISPQSVRQRLASADPPLLVCAYANDEKCRNNAIDGAITYNEFKG
                     LDGLPQGREIIFYCA"
     gene            10531..12966
                     /locus_tag="PLU99_06375"
     CDS             10531..12966
                     /locus_tag="PLU99_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16720.1"
                     /translation="MRLCLLITVIVCGAFGAAELCAQVVSGDVTAAGFRTSQTAMPGY
                     AVRAGHWIPIQVPLSAQGTTPFQGQLRLEAPDLDGDLVSYREGPVALNFEAGAKRAWC
                     YAVWSHRTGAAARPVTLDVLTDAGLPVSELNVPDVEVINNDALLLLDISNQPVTRLRG
                     ALLTPGGTLERGDGVRPFYRDIVVAAMPAGDLPERWFGLEAVDIIVWDKPTPADPAVA
                     SKLDALQQWVRSGGQLVVGLGASWPVVQKSGLAELLPVQSGGRGGGQTIETDRLDEFF
                     RRFGAGSKAAAGERVFRAPISVAAVEARPGAVRVLRSPVADGAEVDLIAAHWIGSGSV
                     IALAAGLDDLTQAGPNQAFYSQLLGLNPTTPEFLESELKSIQATLGLQRHALYDSITR
                     PIALTGAGTLLVFAAVAFVVVYIALATVASWWWLRRHRLATLGWTTFAAIAVVASAVS
                     LLTVSVTRGLSRGLATFSFVDLEAGSRAARGTCYFGYSSPLRTSVDLSLAGAGCFLRP
                     LSPGPAPTSWYATPQRYAATPANALLESTPLRATLKQFEGLWQGELNGAVRCELTIDR
                     RSGELTAGSWLQNDLDVPIAGGYLLYTDPRLPGTPLRAAGQTATWSGWKDVPPALNII
                     ALPVPALKPGEKVSGLGQEEYARLPAARQRWQSLRERKFNAWPDLLTLYDLQQDWVGA
                     RTRAPAAKWDQTVREALLASTRNFYLHVQESNDKFEQSNGRIITTDGLMDVDVCHWLM
                     QGPLKGAGGRPDVIAGQGFLLLYSDVPGPAQLQREGKPVKPNRGACLYRVRVPLQLVG
                     TPPAGPPETGNEL"
     gene            12966..>13879
                     /locus_tag="PLU99_06380"
     CDS             12966..>13879
                     /locus_tag="PLU99_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16721.1"
                     /translation="MIQTINLTKRYGKLVALNNLHLNIEEGECFGYIGPNGAGKTTTI
                     RILATLLQPTWGEARVCGHVVGYESRKIRPLIGYVPDFFGAYEDMVVREYLEFFASAY
                     NIVGKKRDQIVGDVLELTDLRHKEDALVDSLSRGMKQRLSIARVLLHDPKILLLDEPA
                     SGLDPRARIEIRELLKELHRMGKTILISSHILPELADLCTSIGIIEQGELIYQGSVAE
                     ALRRARLGTVVHIATPDDQERARQILCKLPGVEKAELRDGQVVVSLTADGSDFSFIAR
                     AMLKNDLRIREIKQEEVNLETAFMRLTKG"
BASE COUNT         2463 a         4409 c         4504 g         2503 t
ORIGIN      
        1 ccggtattcg cggggggtgc aactgaggtc gccggcgttg tagcggccga tcctgccgtc
       61 gcgccggcca gtgccccagc taccgaaagc caggccagac tgccgcgcgt cgtcgatctc
      121 ggggccggca agtgcaaagc ctgtaaagac ctcgcgccga ttctcgagga gctcaagaaa
      181 gaatatgctg gccgcgtgga agtcgagtac attgacgtct ggaagaacaa agccgcggcc
      241 gagccctaca agattcgtat cattcccaca cagatcttct acgaccgtga aggcaaagaa
      301 gtctggcgcc acgaaggctt tcttcccaag gccgacttca tagccaaatt cgccgaactg
      361 ggagtgaagt aatgaccgta gattccgccc cgccacccgt agtgagcgtt cccgccaaac
      421 cacggcaggc ttgggggcgg acgctctggc gcggcctgaa gtgggcggtc gggttggccg
      481 ttgccgctgc gattgcttac tggatgtggc tgtcgcccat gccggtgata gcacatgtcg
      541 tgcagcgcgg cgaagttgtc gccgaggtaa tgggcaccgg cacgctggaa tcgcacatta
      601 gtgccagcat cagcccgaaa atctcggggc tgatcaaggc agtcctcgtc gatcagggag
      661 atcgggtcga ggccgggcag gtgctggtgc aactcgacga tggcgacttg agccggcaag
      721 tcgaggtggc cgagagcgcg ctttctgcag cacacgccgc tgttgaacgc cggcaggcag
      781 accgggcacg cgcgctggcg gttctcgatc aggcgcgctt cgattttgac cgcgtcgcca
      841 atctcgtgaa gctcgggacc ggctcaacca ctgagttcga agatgcgcgc aagacgctgc
      901 gcgtctccga agccgatctc agctcgtccg aagcggcttt ggtcgaggct cagaagcaag
      961 tcgtcaccgc catgaagacg ttggagctcc accagacgag gttggcggat gcttcggtgc
     1021 gcgccccgtt cgccggcctg attacgcgcc gcgaccgcga cccgggggac atcgtggttc
     1081 cgggtagttc aatcctggcc ctggttgcga tggacgagct gtggctgagc gcctgggtcg
     1141 acgagaccca aatggcaaaa ctgcaaccgg gccagccggc ccgcgtcgtg ttccgcgccg
     1201 cgcccggccg atcgtacccg ggcgcggtgg cccggctggg gcgcgaggtg gaccgcgaaa
     1261 cgcgcgaatt cctggttgac gtccacgtcc atgagctgcc tgagaattgg gccataggcc
     1321 agcgcgcgga aatctacatc gagaccggac gcaaggcgga cgcagtcctg ctgccgtcgc
     1381 gctttattgg ctggcaagac ggcaaagccg ggacatttgt ggagagcggc ggacgggccg
     1441 cttggcgccc gttacgcctg ggcttgcgcg gacaggagtc ggttgaagtc atcgagggac
     1501 tcagcccaaa tgaacgggca atcgtgcccg ccactcagaa aacctccttg aagccagggc
     1561 agcgcgtaaa ggtcccagcg cccggcccgc acggctccgc ggcgaaacag ccatgaacct
     1621 cgcgcgcaaa gacgtctggc acaaccgcgg ccgtttcgcc ctgacgacgc tcggcctggg
     1681 cacgctgctc atgattgtca tgggcatggg tggcatctac cgcgggctga ccacagaggc
     1741 gacccaacta gtcgacaagg tgggcgtgga actatgggta gtgcagcgcg gcacacgcgg
     1801 ccctttcgcc gaaatctcgc gcgtacccaa gacgcttgtc gaccgcgtgg cagccgtgcc
     1861 cggagtggcc gaagcgcggg ccttcgtcta tcacaccatc cagcgcctgc gtgccggcaa
     1921 accgctgcgt gcctcggtcc tcgggctgga ttggccaacc gacaagggcg agtggctgcc
     1981 gctgagcgcc gggcgcccgc tcgcgcaggc gcatttcgag atgatcgcag accgctcgct
     2041 gggcctgaca ctcggggagc gccttccgct gggcaaggaa atctataccg tggtcggcct
     2101 gaccagcggt atgagcgatc cggcgggtaa cggctttctg ttcctcaccc tggcggattc
     2161 gcaggcggtt cagttcgacc agtcgcccga aggcattcgc ctggaacgcc aggcgcggcg
     2221 cgcgcgggcg gagcagatcg acctgggcaa cacccagccc acgatgctgg agcgggcggc
     2281 cgggcccggc tcacagctcc cggccttgcc gccaaaccag atcagcgccg tcatgacccg
     2341 cctggcgccc ggcgttaacg aggctgcggt gaaagcgacg atcgacggtt gggccgatgt
     2401 gtcggtctat acgcgggccg atgaagccaa gatgctgctg atgggagtgg tcgagcgctc
     2461 gcggcggcag ttgggccttt tccgcgtact ccttacgacc gtctccggca tcattatggc
     2521 gctcatcctg tacacgctca cgatggacaa gctgcacgat atcgcgctgc tgaagctcat
     2581 cggggcatcg aattgggtca tcgtcaaatt gatcatgcag caggccatcg cacttggagc
     2641 tttggggtat gcggtggcct ggttgctggg gcaaaaggcc tatccgtact tcccgcggcg
     2701 ggtgatcctg accccggatg acttgctgat gttggcgggc gtggtgctgg gcgtatcggt
     2761 tgtggcaagt ctcgtgggta tctggaaagc gctgcgcgtg cagcccaacg tggcattgct
     2821 gggatgagct gctgctgtgc gaaacgccgc cattgaaaca cacggtctga ccaaggtata
     2881 cggcagcggc cacaccgagg tggtcgcgat gcgcgacgcc tcgctgtgcg tggcgcacgg
     2941 gcaatgcgtg gcgctgatcg gccccagcgg cgcgggcaaa tccacgtttc tcacggccgt
     3001 cggcctgatc aaccccccta ccgcgggtcg catcctgatc ggcggccggc tggtgatgga
     3061 aggtccacgc gcgctgacga acccgcgcac gtttcgacgc aagcacatcg ggtatatctc
     3121 gcaaaaggcg aacctcatcc gattcctgac cgcccgcgag aatgtccagg tcgcaatgga
     3181 gctgaatggc gagtcgtccc gagcggcccg tgcccgcgcg ctggagctac tggaatatct
     3241 cggggtgcgc gaacgcgccg ccggctatcc ccccgagctc tccggcggcg aacagcagcg
     3301 cgtggcagtg gcccgcgctc tggccaaccg tccgagcgta atcctggccg acgagccgac
     3361 cgccgcactc gacgggccgc gcggccgcca ggttatggag ctgttccgcc gcgtcgcgca
     3421 cgagcagggt gccgctgtga tcgtggtcac gcacgaccac cgcgcgctgg acgtgttcga
     3481 cacgatttat gaaatggaag atggcgtgat ccggtccggc gcggccgagc tgtctgccgc
     3541 gagtacattc agcgggggaa aggcgcgccc ctgaaaccag cggcctcgga tgcggctgca
     3601 atgggataag acaaaatgct cgagtcgttt gccgagaacc tggccgaaat aattaaaacc
     3661 aacatctggc tggcgccgct ggcggcgctg gtgggcggcc tgttgaccgc cgccaacccc
     3721 tgcgtgctcg cggccgcgcc gctcatggtc ggctacgtcg cggggcagga aaagcgcacc
     3781 gtcgcccgtt cgttcctcct gtcgctgacg ttcgcaatcg gcctgacgat caccttcggc
     3841 ctgatgtggt tcagcgtgtg gtccgccagc agtctgctgc cggccagttg gtggaagtac
     3901 atcgcggtgg tcgtgtgcct gctgatgggt ttgcacctgc tcggagttct gcacatccgc
     3961 atcccggctc cgaccggcct gcagccgaag cagaagggct tcatcggcgc gttgctgctc
     4021 gggttgttgt tcggcctgat ctcgatcccg tgtgccggac caatcttgct cgctctgagc
     4081 gccgtcacgc cgctgtacgg cctggcattc ggcatcctgc tgctgtccgc gtacagcctg
     4141 ggccactgcg gcctggtact ggtcggcggc acgtcgatcg gccttgtgca aaagctcgcc
     4201 gactcgaaag gctggacgcg cggcacggac gtgctgcggc ggctggccgg cgtgctgatt
     4261 gcgggcgtcg ggctgtacct gctgttctca tagggtgcgc tcgtaggtgc gtcctcgtag
     4321 ggtgccccct cgcgcgatct tcccgacaac tgataactga gaacggtcaa ccgcgaatag
     4381 gggcaattca agatcaaaag gggaaccgga caagagcgcg cggctccggc gtctcgtaag
     4441 tgcttgattt tacaggggtt agaatggagc cgatgagatt cgaactcacg acctcttgca
     4501 tgccatgcaa gcgctctccc aactgagcta cggccccagg agtaaacatc atattcagtt
     4561 gtcactcgtc agtttgcgcc gccggcgcca aactggcgtt cacctcaacg tggtcagggg
     4621 cggacttgaa ccgccgacac acggattttc agtccgttgc tctaccaact gagctacctg
     4681 accgccgggc gactcaggct cgccctgatc ttacgaccag tcgcgcgaat ttagggcacg
     4741 ttgccgttct agtcaagggc gtccgcccaa ctgccacctg cccctgccca cctgcctccc
     4801 ctgcccacct cgaactcgcc gcctcgaacg ccgtctcgaa cacgccttct cgaactcgcc
     4861 ttccgacggg cttacccctt cgaaaccggt cccatagact caggacagct cgtcacgcac
     4921 ccgctccagc agccgcttcg tcgcccgaat cccatccgcc tcggccaagc gcgacccctc
     4981 gtattcaatg ccgatgtagc cgtgatagcc ggcccgcagg acgatgccga tcatccgccg
     5041 gtagtctatg tcaacttcgt tgcccgattc gtcgaaatcg tgcgacttgg cgctgaccgc
     5101 cttggcatac ggcatcatct cagccacgcc cgcgtaacga tcatagcccg cgttgtcggc
     5161 ggcattcggc ggcagaaaat tgccgaaatc cggcagcgtc ccgcaacgcg gatggtccac
     5221 gcgcttcatc agcgccacga cccacgcgcc gttcgaagac agcccgccgt ggttttcgac
     5281 cagcacgttc aaatcgcgct cggccgcaaa ctccgtcagc cggcgcaggc cgtcagcgac
     5341 cagcgcgagt tgctcttccg ggctgccgga gctctgcgcg ttgacgcgaa tgctatggcc
     5401 gccaagcagt cgagccgctt cgacccactt gtagtggttc tcgaccgtgc gccggcgagc
     5461 ggcctcgtcc ggatcgccca aagccccctc gccatcgacc atgatcagca ggctgcgaac
     5521 gccggcgtca accgcgcgcc gccgcagctc gcccacatag tccgcgccgg cgctcttgaa
     5581 aaacgtgctc acgtattcca ccgcttcgat gccgtagtcg cgcttcgcag cctcgggaaa
     5641 atcaaggtgg tcgagttgac ccgccccgag ctggcggtgc agcgaccact cagcgagcga
     5701 aatcttgaac agcggctcga ctcgccccgc gtccgaacca gcggccgtga ccggctgcgc
     5761 gaccggttca tttcgcgccg gcgccgtgcg gcagccggcg gggccgagca cgaccgctcc
     5821 aagcgtggcc gcgagagttt tcaaagcgct ccgccggccg atgccgttcg agacacccgg
     5881 tagttccttg atcggccgcg agtcgtcgac gttccgagcg ttcatgcacc ctccgtgagc
     5941 acaattacgg gaataaggcc ggttctctcc tccggctcag cacgagccgg tgccgctacc
     6001 ggccgtcgta gagtcgcgtg ccgagcagtt cgtccaaggc ggggagcgcc aggattttct
     6061 tgaccgtttt ttctatgtcg tactcgacgc gcacgaactc gatttgctcg atggcgcagc
     6121 tcagcgccgc cgccaccagg ctctccgtgc ccgtgccgag cacgtcgttc tgccggtaga
     6181 ccaggacata gctggcccgc ggatcgcggt cgcgcggctg cccgacgctg ccgacgttca
     6241 cgatcgcccg ctcgtcctcc acctcgtaga agggcgaatc cggcaactcg ttcggcgggt
     6301 caaaataagg atcatccagg aaaacgcccg gctgatgcgt atgcccgacg aagcacagcc
     6361 gccggtctaa gcggtcgaaa ttcgccacca ccttctgcgg attggtgaaa acgtcctccg
     6421 ggaagatgta ctcgttgatc gggcggcgcg gcgaagcgtg aacgtacaga atatcctggt
     6481 cttggaagcg caccggcatg ttgccgagga actgccagcg cgcgttccgc agctccttgt
     6541 cctcctcgtc ttccagtaac tgccgcgtcc agaagcacgc ccgctcggcc gccacgttga
     6601 agttgcacgg ctcgtacagc agagcatggt catgattgcc catcaggcac actcggcacc
     6661 gttcccgcac gaggtccagg cattccttcg ggttagggcc gtacccgacc acgtccccca
     6721 ggcagacaat ctccgttacc ccgcgctcct cgatatcgtc cagaacagcg cgcagcgcct
     6781 ccaggttcga atggatgtca ctgataaccg cgaatgctct gtctggcatc gcccgtcacg
     6841 gcggagagtt acactgagtc aaggcacctc cggcaccgcc ggccgttccc cggacgggcc
     6901 accggaccag ccgcgctcgt cggctcgtcg cacaaggcgc agtaagaata gacccaatcg
     6961 cagggcaaat caattagccc tccggaggtt tcgggccgtg gctacaaaag cgtacccgcc
     7021 cagccaaggc tcgtaactca ccggcccgag cacttgggcc agccgctgcg gataggcgcg
     7081 gccggtggcc acgtgattcg cccgcagcca ggcgcgtgcc agccacccgg ccgggcccca
     7141 ccgctcaaat cggccgaaat ccaggatgac gagccgcccg ccgggaagaa ggtggtccca
     7201 ggcccgggcc agggcccgtt cccagtcggg gatcatggac aagctgtatg cgaaaaggac
     7261 ggcatcgaac cgttcaccga gcgccaaggc agtcgcatcc gctgatacca ggtcgacatt
     7321 gagccaaccg cgacgctcga tgaggccgcg agcccgcccc agcatgtcgc gcgagaagtc
     7381 gacgcccgtg agcctgccgg cctgcggatc aagctgcttc agaatggccc cgaagttcag
     7441 cccggtgccg cagccgattt ccagcacgtg gctgtcgggc cgcagttcga ggcgctggat
     7501 ggccagtttt cgacggtgga ggacggccca gcgcgtccag tcgtagatcg ccgcgtgaaa
     7561 ccggtaatag cgctgaacgg gcgtctgctg cacgttcaat ctctcccgag ggccggcgcg
     7621 ggccagccgt aggccggccc gaggcgcggc gcgggccaga cgtaggccgc gctgctgcac
     7681 attgccacgt tcacactttc ccacggtcgg gcgcgcgtgg gaagctggga aagtgaggaa
     7741 tgtgggaagg acccgcgaac aggcccggct ggccagcctc aaggcggaac ctcgtgcacg
     7801 ccttcacgtc cgcggattgc atgcgtcggc cgcccttcca gttcgatctt cgcttcggca
     7861 atgtggagcg ctgggcaggc aggcgtcgag gccacgcggg gcggcggcgt gcggcgatgc
     7921 caccccaggt gctcaagcgg tctgataaaa aggcctgtgg gatccggtct tgcgggcaga
     7981 tgatcagata cgcatgcttt ttgtgcactc gattttgata acctgaggca agggggcacg
     8041 ggggcggggt tgaagctgcg gcattataag atgatggcgt gggaagcatt ggcgtggctc
     8101 cgtgggtagt tctcagttct tagttgttag ttgttagttc tggcatcgtg ggcctccggc
     8161 gccggtctgc gcgctttcct agagatcata ggacgttatt cgaggcgggg ggcgcccggc
     8221 ggttttcggt cgcgggggtg gattgttggc ggttgggcgt ggttgtgtgg cagtgacggg
     8281 gtgtggccgg ctttcagcta tgagctttga gccggaattc agcggattgt gcgtggttgc
     8341 ccgccaatgg cgaagagtgg gcaaaaggca cgctgcgaac ggcgagcgct ggtcgtcact
     8401 tcggtggcct tctaatccgt ttcccgcttt tggagtctct gctgaaattc cctcgtgcgc
     8461 acgtccgtat caatccaatt gacggttagg tcgatcaact ccttcaagtg ctcgatatcg
     8521 aaatcatcgt actggcgcat gtaatgcgtt tcgtcattgc ctagccaaac cgcccggctt
     8581 gcagtggtct taatgttctc atcggtaacg aagttgttta tgcaggcccc aagggcagtc
     8641 ttctcgatgg aatcggcttt cgaaggctcg agaattttgc agtagtcttt gaccaggaat
     8701 tccagtgcct ttcgatagcc ggctccggca acctgcttca gcttcaggcc ttcggcaacg
     8761 gcggattgcc cgtagatcgc cgcgaatccc ggcgaggtct ttctcacgct cgccctcacc
     8821 tgcggcgcgc gaaagatcgg tgggccaatt tctctgagcc ggaaatgacg ctcagaagcc
     8881 cgcccgggta ttgtttcata attggcaatg aatagatgct ggcaagcgtt gttttggcac
     8941 tggaagatca agtccaattc caaggcgcgc ccggtttcga ttgcagcagc ccaaagggga
     9001 ttggatgtcg tggcatgccc gcaatgcgga cattcgtccg cataaaggtc gacctggcaa
     9061 tcgtaaagct ttccgaactt cgtctgtgct ctcacggcca gcatggcgcc gatgatatac
     9121 gcccacggcg cgtcgcacaa cgggaccgag accggccacg cagggagctg gcgacaacct
     9181 ggcgggaagc gtgcgacagc accgcccgga ataaaacggg ctcactgtac aatgaacgtc
     9241 gctgaggagt gcgctcatga tcgagtttcc gggcgaaaag gttcgcgaag tggcgggacg
     9301 tgtgtaacgg ggacgcagcc gtgccacact gagctgcgtc cctgtttcac acggctaagg
     9361 tgcgtccccg tttcacaccc gtctcacact ggctatgggt gcgcttcggc catcatgccg
     9421 cgccaaccct tgataccgcc atcgagaatg taaacgttgg tatagccttg gctcgcgtat
     9481 tcggctgccc gacgggcagc agtgccatcg cggggtcagg cacagtagaa aatgatctca
     9541 cgcccctgcg gcaggccgtc aagccccttg aactcgttgt acgtaatcgc gccgtcaatg
     9601 gcgttgttgc ggcacttctc gtcgttggca taagcgcaga ccagcagcgg cgggtcagcg
     9661 ctcgccagcc gttgccgcac cgactgcggg ctgattcgtt ttggcccgcc aggattttct
     9721 gccgcgacgc tcggcgccgg tggtgtggcc tcgaccgccg aaaccggcgt ggcagcgggc
     9781 tgcggcgtca cggcgctggg cggcggcgcg acggcgctgt cctgagccgc gacggcgctg
     9841 tcctgcgggg ccacttccgt gggctgcggc gccgcatgcg gcgccacggc gctggcctgc
     9901 ggcgagaccg ccggagcggc ctgctgcgcg acagggccgg ccgatggggc ggaattcccc
     9961 tcaccggagg cgcagccggc cagggccaca agtgcagtgc acaacaacga tgcacaccag
    10021 attttcacaa cacgtgtcac catacagctc cagaatgttg ttttgggcag agagcagcct
    10081 cgcatcccga ctcccagaac gcccccgata cacatagtcc tcttacagca tagtcttatt
    10141 tacagcgagg tcatgacagt ttgttaaatt gcacgtgaca ggcccgtaag ccgagccgcg
    10201 ggcttcaggc atcccgccaa caagtcggcc gaagccagca attggtgtgt gaaacgggat
    10261 gtgaagcgcg agcgcagcta gcgctagttt cttagcctgg caacaatcgt tcgccctccg
    10321 ggcgaggaca cggggacgca ctgggcgccc acatcccggg ggcctgcgtg ccacgagtgg
    10381 cgtggacggc ccccccctgt gggcgcgctg gctggatggc gtaaactacg ttgaacgccg
    10441 ggcttactgt gacccgggca tgctgtaacg cgggcccagt gttgacccgc ggcagaggat
    10501 gtatgccgta gaacgcgatc gggccatcga atgcgactgt gcctgctgat aaccgtgata
    10561 gtctgcgggg ctttcggggc cgccgaactt tgcgcccagg tggtttccgg cgacgtcacc
    10621 gcggccgggt ttcggacttc gcagaccgcc atgcccggat acgcagtgcg ggcgggccat
    10681 tggattccca tccaagtccc gctcagcgcc cagggcacca cccccttcca gggccagcta
    10741 cggctcgaag cccccgactt ggatggcgac ctggtttcgt accgcgaggg gccggtggcg
    10801 ctgaatttcg aggccggagc gaaacgggcc tggtgctacg cggtgtggtc tcacaggacc
    10861 ggggcggcgg cccgccctgt cacgctcgac gttctgacgg acgccgggct gccggtcagc
    10921 gagcttaacg tcccggatgt cgaggtcatc aataacgacg ccctcctgct gctggacata
    10981 agcaaccaac cggtcactcg gctgcgcggc gcgttgctga ctcctggcgg aaccctggag
    11041 cgtggcgacg gcgtacgccc cttttaccgc gacatcgtcg tggccgcgat gccggccggc
    11101 gatttgccgg aacgctggtt cggcttggaa gcggtggaca tcatcgtgtg ggataaaccc
    11161 actccggccg acccggcggt cgcgtcgaaa ctggacgcac tgcagcagtg ggtgcgcagc
    11221 ggcggacagc tcgtcgttgg cctgggcgcg agctggccgg tggttcagaa gagcggcttg
    11281 gcagagctcc tgcccgtgca atccggcggg cgtggcggag ggcagacgat cgagaccgac
    11341 cggctcgacg agttctttag gcgctttgga gcaggctcga aagcggcggc gggggaacgc
    11401 gtgttcagag caccgatttc ggtggccgcg gtcgaagcca ggcccggcgc ggtgcgcgtg
    11461 ttgcgcagcc ctgtcgccga cggcgcagag gtggacctga tcgccgcgca ctggatcggt
    11521 tcgggaagcg taatcgccct agcggccggc ctggatgact tgacgcaggc gggcccaaac
    11581 caggcgtttt acagtcagct tctgggcctg aaccccacca cgccggaatt cctcgagtcc
    11641 gaattgaaat cgattcaagc gacactcggg ctgcagcgtc atgcgctcta cgacagcatc
    11701 accaggccga tcgcgttgac cggcgccggc acgctgctgg tgttcgcggc cgtggcattt
    11761 gtcgtggttt acattgcgct ggcgaccgtg gcgagctggt ggtggctgcg ccggcaccgg
    11821 ctggccacgc tgggctggac gacatttgcg gccatcgcgg tcgtggccag cgcggtcagt
    11881 ctgctgacgg tgagtgtaac gcgcggtttg tcgcgtggtt tggccacgtt ctccttcgtc
    11941 gacctggagg ccggctcgcg tgccgcgcgg ggcacctgct acttcggcta ctccagtccg
    12001 ctgcgaacca gcgtggactt gtcgttggcc ggcgcgggct gtttcctacg gcccctctca
    12061 cccgggccgg cgcccacaag ctggtatgcc acgccgcagc gctacgcggc cacgcccgcc
    12121 aacgccctgc tggagagtac gccgctgcgg gccacgctca agcagtttga aggcctctgg
    12181 caaggcgagc tgaatggcgc ggttcgctgc gagttgacca ttgatcgccg cagcggcgag
    12241 ctcactgccg gcagttggct gcaaaacgat ctggacgtgc caatcgcggg cggatacctg
    12301 ctttatacag acccgcggct gccgggcacg ccgctgcgcg ccgcgggaca gacggctacc
    12361 tggagcggct ggaaagacgt gccgccggcg ctgaacatca ttgccctgcc ggtcccggct
    12421 ttgaaaccgg gcgaaaaggt gagcgggctg ggccaggagg agtatgcacg cttgccggcc
    12481 gcccggcagc gctggcaatc tttgcgcgag cggaagttca acgcatggcc ggacctgctg
    12541 acgctctatg acctgcagca ggattgggtc ggcgcgcgga cgcgcgcccc ggcggccaaa
    12601 tgggatcaga cggtgcgcga agcgctgttg gcctccactc gaaacttcta tctgcacgtt
    12661 caggaaagca atgacaaatt cgagcaatcc aatggccgga taatcacgac cgacggcctg
    12721 atggatgtcg acgtctgtca ctggctgatg caaggaccgt taaagggcgc gggcggccgc
    12781 ccggacgtaa tagccggtca agggtttttg ctgctgtatt cagacgtccc gggcccggcg
    12841 caattgcagc gtgagggcaa gccggtcaag cccaaccgcg gcgcctgcct gtaccgcgta
    12901 cgtgtgccgc tgcagcttgt gggcaccccg ccggccgggc cgcccgaaac ggggaacgaa
    12961 ctgtgatcat tcaaaccatc aacctgacca agcgttacgg caaactggtg gccctcaaca
    13021 acctgcacct caacattgag gagggcgagt gcttcggcta catcggcccc aatggcgcag
    13081 gcaaaactac caccattcgc attttggcca ctctgttgca gccgacgtgg ggcgaggcgc
    13141 gcgtctgcgg gcacgtagtg ggctatgagt cgcggaagat ccggccgctg atcggctacg
    13201 ttccggattt cttcggggcg tacgaggaca tggtggtccg cgagtacctg gaatttttcg
    13261 ccagcgcgta caacatagtc ggcaagaagc gcgatcagat cgtgggcgac gttctggaac
    13321 tgacggacct gcgccacaag gaagatgccc tggtcgattc actgtcgcgc ggcatgaagc
    13381 agcgtctgag cattgcgcgc gtgttgttgc acgatccgaa aatcctgctg ctggacgaac
    13441 ccgccagcgg gctggatccg cgggcgcgca tcgagattcg cgaactgctc aaagaactgc
    13501 accgcatggg caagaccatc ctgatcagct cgcacatcct gcctgaactg gccgacttgt
    13561 gtaccagtat cggcattatc gaacagggcg agttgatcta ccagggctca gtggccgagg
    13621 cactgcggcg ggcgcgcctc ggaactgtgg tacacatcgc cacccccgac gatcaggagc
    13681 gggcgcgcca aatcctctgc aaactgccgg gcgttgaaaa agccgagctg cgcgacgggc
    13741 aggtggtcgt cagcctgacg gcggacgggt ccgatttcag cttcatcgcc cgggccatgc
    13801 tgaagaacga tttgcgcatt cgcgaaatca agcaggaaga agtgaatctc gagactgctt
    13861 tcatgcgtct gacgaaggg
//
LOCUS       DAOSYS010000075        13859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3726_length_13859_cov_5.151985, whole genome shotgun sequence.
ACCESSION   DAOSYS010000075 DAOSYS010000000
VERSION     DAOSYS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_13859_cov_5.151985"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PLU99_06385"
     CDS             <1..1257
                     /locus_tag="PLU99_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOO16722.1"
                     /translation="TTANVDQADLDGDGLGDMCDPDIDGDEVPNASDNCPAVANPDQA
                     DLDDDGIGDACDPDIDNDTVVNEQDWAPFDRFRCSDADADGCDDCTSGIDDPANDGID
                     TDGDGICDLGDPDDDADGVPDAEDAYPQNPFACGDTDADGCDDCNLGYFDPLNDGTDL
                     DGDGTCDFADEDDDGDGVPDEFDPDPSDRFTCGDTDFDGCDDCSTGAFDLLDDGPDAD
                     GDGLCDAGDPDDDNDGLLDADELAAGTDPLDPDSDDDGLLDGVEVDIGSDPLNPDSDG
                     DGLLDGSEVTAGTNPLKADTDEDGVNDREDPLPLDPGVPPTYLVTECRQTAAAILAMD
                     QSKFSGPNNITRKARRTLLACGVTAAANAISARCYLVAAVELLAVDLLIDGYSCPADW
                     MPPSPEKTWLKEVVELELELTLYLLD"
     gene            complement(1653..>4192)
                     /gene="alaS"
                     /locus_tag="PLU99_06390"
     CDS             complement(1653..>4192)
                     /gene="alaS"
                     /locus_tag="PLU99_06390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOO16723.1"
                     /translation="LTEVWGLDKTRLHATYFQGDEAEGLEPDLEARDLWASVTDIDPT
                     HIHPGNKKDNFWEMGETGPCGPCSEIHIDLTEDKSGRTLVNAGDARVMEIWNLVFIQF
                     NRGPDGKLSPLPARHVDTGMGFERVTALLQGKKSNYDTDVFAPIFAAIQAVTGAPAYG
                     GRLEAKEQGIKGSKDQVEDQAVMRDVAYRVIADHARCLTFALTDGAVPDREGRGYVLR
                     RILRRAVRYGWQYLETHEPFIYRLVPSIVETMGAAFPELRSNPQRVMDLIREEEDSFG
                     RTLERGIELFEKAAALAREEHHGVIRGEDAFKLHDTYGFPIDLTEIMAAERGLRVDIG
                     EYERLMEQARQRARAAGKLAETRFGQVPGEVIEHAGPTDDRPKYETDRLVATVTAAVR
                     FEGDGTAKALAPGEALRPGDRAAIITDRTCFYAEQGGQIGDAGRIESPEIRFDIEDTA
                     RAGEAVLHLGTMVQGHISAGQTVGLVVDQRRTLTKKNHTATHVLNWALREVLGDHVQQ
                     KGSLVDQDKTRFDFSQPRPLTADELAAVERLVNERIAARLPVYDKYVPQQQALQIRGL
                     RAVFGEKYPDEVRVLSVGTPVDDLLGNPENPRWREVSIEFCGGTHVKNTAEIEHFVLV
                     SEEGVAKGIRRIVGVSGPTARLIDEMGAALMRRAEELKTERPADLPQRLSELQHSVTS
                     ATLPLKHRLRLRELMGELQALVREQQKAAAADAAGVVESKITELLKSAPKIGETTALV
                     AEMPEVPIEQLKIGADLVKQKTHSAAVLLGMRNGDRATLLAAMSNDLIKKGLKAGDLV
                     KAVAPLVEGGGGGPPTMAQAGGKNPAKLGEALEAGRKWLESKLSGEK"
     assembly_gap    4193..4292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4738..4938
                     /locus_tag="PLU99_06395"
                     /pseudo
     CDS             4738..4938
                     /locus_tag="PLU99_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
     gene            complement(5169..5939)
                     /locus_tag="PLU99_06400"
     CDS             complement(5169..5939)
                     /locus_tag="PLU99_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16724.1"
                     /translation="MVEAPGVRDAQAVAIRIEALAHRYPDGNNALCGVDLTIQSGQRV
                     ALVGPNGAGKSTLVRHLNGLLTPTAGRLFISGLEVVPANYTAVRRRVGFVFQDPNDQL
                     FCPTVLDDVAFGPLHLGLPIEEVIERVQGALRRVGLPGFERRVPQRLSAGEKRLVAIA
                     TVLSYEPEILVLDEPSAALDPRNRRRLIELLKGIGGTQLVATHDLDLAWELCERAVLL
                     AGGRVVSDGPTRTVLSDRQLLESAGLELPLCLENQRGV"
     gene            complement(5962..6924)
                     /locus_tag="PLU99_06405"
     CDS             complement(5962..6924)
                     /locus_tag="PLU99_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16725.1"
                     /translation="MDAIRTHQLTRSFGELVAVDALTLNVTPGEIYGLVGPDGAGKTT
                     AMRLLTGILEPTAGQAWVAGHDVVREAEALKEKIGYMSQRFGLYPDLTVLENIQFYAD
                     IYDVPRRKRNEKIEALLGFSQLTSFKKRLAGRLSGGMKQKLGLACALIHTPQILFLDE
                     PTNGVDPVSRRDFWRILYQLVREGVTIFVSTAYLDEAERCNRLALLHRGRLIAVGTPD
                     EVKRLMRGTIIEIRCSRAREATALLREKLPGATISLFGDRVHAVTNDPQETAAAVGAL
                     LREAGFGYHEVQQVEPTLEDVFVSVLGEQPHPSAASEADRDARQ"
     gene            complement(7017..8171)
                     /locus_tag="PLU99_06410"
     CDS             complement(7017..8171)
                     /locus_tag="PLU99_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO16726.1"
                     /translation="MPTRMKAALTLFLLSIAAASLGAFFHNRGGAEPNVIYVSGNIEV
                     TDAEVSFKIAGRLDKRVASEGQLIQRGDLVALLDTAELESQVAMRNAELASAKAVLAE
                     LEAGARPEEIAEAEAALDRARAALAELEAGSRPQEIAVAEAAVASARADLGEAEATYD
                     RIQGLYERSMAAEQEYVSARARRDVARARLDEVEERLKLVREGPRSEQIAAARAAVEQ
                     AEQHCKLVKQGPRREAIDQARAKVEQAAAAVRLAQTQLDYARLVSPLSGLVLSQNVED
                     GEYVAPGTPVVTVGDLEHPWLRAYINETDLGRVKVGRKVRVKTDSYDDKVYEGRVSFI
                     SGQAEFTPKNVQTNKERVKLVYRIKIEIANPNMELKPGMPADAEIELDAK"
     gene            complement(9213..9452)
                     /locus_tag="PLU99_06415"
     CDS             complement(9213..9452)
                     /locus_tag="PLU99_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HOO16727.1"
                     /translation="MPLIEVKLIEGVFSPKQKQQMIAKLTDAMVSIEGENLREVTTVI
                     VEEVKSGDWGIGGKSLSTADVKAMAAGGVASAVQR"
     gene            complement(10093..10971)
                     /locus_tag="PLU99_06420"
     CDS             complement(10093..10971)
                     /locus_tag="PLU99_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOO16728.1"
                     /translation="MLDFFHALRDPDSVFLVYALVTGILASIACGIVGTYVVVRRITY
                     IAGGLAHCVFGGLGAAYYLERVHHWQVYNPIYGWQPLDPLHGAVVAALLAAMIIGFVS
                     LRARQREDTIISALWAIGMAVGVLFVYLTPGYSQDLMSYLFGSILIVSPNDVWFLAVL
                     DALIIAVALLFYKQFLAVCFDEEYARLRGLNVEFYYLLLLCLVALTVVLLVSVVGIIL
                     VIALFTLPVAVAGQFTRIFWRIMAVAALLSMVFVTLGLALGYSLGLQPGATTVLVAGA
                     TYLLVLVASQARRLAR"
     gene            complement(10979..11758)
                     /locus_tag="PLU99_06425"
     CDS             complement(10979..11758)
                     /locus_tag="PLU99_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOO16729.1"
                     /translation="MTAPANNNPSPSAPPETVIAVERLTFAYQGAPVLEDVTFSIAAR
                     EFVSIVGPNGGGKTTLLKLMLGLLQPQSGTIRIFGRPPLEVRQRIGYVPQHAQFDPQF
                     PVTALDVVLMGRLGISATFGPAGRADRRAAAQALAEVGLADYARRPLPALSGGQRQRV
                     LIARALASEPELLLLDEPTANLDIAVQGDFLELLHGLNKRMTILLVSHDVGFVSQHVR
                     TVICVARRVDVHATRELNGRTISALYGGPVRFVQHSHGTHE"
     gene            complement(11755..12984)
                     /locus_tag="PLU99_06430"
     CDS             complement(11755..12984)
                     /locus_tag="PLU99_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOO16730.1"
                     /translation="MNRNNRLRANFASAANRRLAAFLLALAAPVLVLTACRQAPPSGT
                     PQTNPAAAPQPEGGQRLGVFVSIPPQAYFVQRVGGDHVQVDVLVGPGQNPHSFEPTPA
                     QISSLAKAAVFFRVGMPFEDGLLPRLRGAFTHLEIVDTRQGVPLRPMEEHEHEHEHDA
                     AHHGHEHDAAAHHAHEHGSEAHHEHPAHHDEEGADPHIWLNPRLVKIQAQTIADALIR
                     LDPANADEYRRNLAAFTAELDALDARIAAALAPVRGQPLFVYHPAFGYFADAYGLHQV
                     PVEIGGKEPSPKDLAELIRRARQENVRVIFVQPQFSTRSAEIIARQIGGAVVPLDDLA
                     PDYMTSMETMAEQVRQALSKPDPQKPEPAKSEPTESAPEPPPTANEPPTGAMPKPRSG
                     AGMPSSPATAPAEPQHP"
     gene            13183..>13859
                     /locus_tag="PLU99_06435"
     CDS             13183..>13859
                     /locus_tag="PLU99_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16731.1"
                     /translation="MRQLEHRRPSWLLDGVIVLLILAAWLPVAWYLTGGLGIVWYDTF
                     RDMAWAENMRAGRIWADPVVAGQTWWYAPGGPLLAAGVARLTGWPVAEVYAASPAWCN
                     GWIPAALYVLARVAWGRGTALLALATVLLGSYWWYTHVAAPIPGIQAVTLMLLVLLGW
                     HLSVTAGRAYTWAAISGVLLALATWYHPMCGILPAFTIALQVLYDALRPRKCRQRLLG
                     RMLVVAGT"
BASE COUNT         2384 a         4386 c         4500 g         2489 t
ORIGIN      
        1 accacggcca acgtcgacca ggctgacctg gacggcgacg gcctgggcga catgtgtgac
       61 ccggacatcg acggcgacga ggtgccaaac gcctcagata actgcccggc ggtggcgaat
      121 ccggaccagg ccgatttgga tgacgacggc atcggcgatg cctgcgaccc ggacatcgat
      181 aacgataccg tagtcaatga acaggattgg gcgccgttcg atcgtttccg ttgcagtgat
      241 gcggacgccg acggttgtga cgactgcact tcgggcatcg atgatccggc caacgacggc
      301 atcgataccg atggcgatgg catttgcgac ctgggcgatc ctgacgatga cgccgatggc
      361 gtgcctgacg ccgaagacgc gtatccgcag aaccccttcg cgtgcggcga cacggacgcc
      421 gacgggtgcg atgactgcaa cttgggctac ttcgatccgc tcaacgacgg gactgatctc
      481 gacggcgacg gcacttgcga tttcgccgac gaagatgacg acggcgatgg cgtgccggac
      541 gagttcgatc ccgatccgag cgaccgcttc acgtgcggtg ataccgactt cgacgggtgc
      601 gatgattgca gcactggcgc tttcgatctg ctggacgacg gaccggatgc cgacggcgac
      661 ggtttgtgcg acgcgggcga cccggacgac gacaatgacg gcctgttgga tgccgacgaa
      721 ttggccgccg gcaccgaccc gctggaccca gacagtgacg acgatggttt gctggatggc
      781 gtcgaggtcg acatcggcag cgacccgcta aacccggata gcgatggcga cgggttgctc
      841 gacggcagcg aagtgaccgc cggcaccaat ccgctcaagg ctgataccga tgaggatggc
      901 gtcaacgacc gcgaggaccc gctgccgctg gatccaggcg tgccgccgac ctacctcgtg
      961 actgagtgcc gccagacagc cgccgcgatt ctggctatgg atcagtccaa gttcagcggg
     1021 ccgaataaca tcacccgcaa ggcccggcgg accttgctgg cctgcggagt aaccgcggcc
     1081 gcgaacgcga tttcggctcg ttgctacctt gtcgcggcag tggagttgtt ggcggtggac
     1141 ctgctcatcg acgggtactc ctgtccggcg gactggatgc cgccttcgcc ggagaagacc
     1201 tggctgaagg aggtagtcga gctggagctg gaactgacgc tgtacttact cgactgatct
     1261 cagcaggctg agcgcgcggc gcgaacttgc cgcggcttgc cgtgaaaaag cggtcaacag
     1321 tcgccggcgg ctgttcggag atggctccga atagccgccg gcccctgttt tggagcgaag
     1381 gtttgataaa caggcgggct tttgaggcag ttagtggggc tcgcgcccgt cgccctaatg
     1441 acaaggtggg aacgtgagaa cgtgggaaag tgggaagggt gaggcggggg cgtggcagcg
     1501 ccggtggcgg cttggccgac attgccgcgc gccgcgtgcc atgcccaagt tctaagcgcg
     1561 ggcatgccac cgccgccggc ttggctgaaa ttgccgcgcg cgattgcaga gtcgaatact
     1621 gcggcgaact atgtcgatga cgcgcgctgc ggctattttt cgccgctcag ttttgactca
     1681 agccacttcc gacccgcctc aagcgcctcg ccaagcttgg ccggattctt cccgcccgcc
     1741 tgggccatcg tcggcgggcc gccgccgcca ccttcaacga gcggcgcgac ggcttttacc
     1801 aggtcgcccg ccttgagccc cttcttgatc aagtcgttgc tcatggccgc taatagcgtg
     1861 gcccggtcgc cgttgcgcat gcccagcaat accgcggccg aatgggtctt ctgcttcacc
     1921 aggtcggcgc cgatcttgag ctgctcgatc ggcacttccg gcatctcggc caccaacgcg
     1981 gttgtttcgc cgattttggg agcacttttc aacagctcgg tgatcttgct ctccacgacg
     2041 cccgctgcat cagccgccgc ggccttctgc tgctccctga ccagggcctg aagttcgccc
     2101 atcagctcgc gcagccgcag ccggtgcttg agcggcaacg ttgccgaggt cacgctgtgc
     2161 tgcaactcgc tcaggcgctg gggcaaatct gcgggccgtt cagttttcag ctcctccgcc
     2221 cggcgcatca gcgccgcacc catctcatca atgagccgcg ccgtcggccc gctcacgccg
     2281 acgatgcggc gaatgccctt ggccacgccc tcttcgctca ccagcacgaa gtgttcgatt
     2341 tcggcggtat tcttgacgtg cgtgccgccg cagaactcga tcgacacctc gcgccagcgc
     2401 gggttctccg gattacccag cagatcatcg accggcgtgc cgaccgacag cacccgcacc
     2461 tcgtccggat acttctcgcc gaaaaccgca cgcaggccac gaatctgaag cgcctgctgc
     2521 tgcggtacgt acttgtcgta caccggcaac cgcgcggcaa tgcgctcgtt gaccaaccgc
     2581 tccaccgccg ccaactcgtc cgcggtcagc ggccgcggct gcgaaaagtc gaagcgcgtc
     2641 ttgtcctgat cgaccagcga gcccttctgc tgcacgtgat cccccagcac ctcgcgcagc
     2701 gcccagttca aaacgtgcgt ggcggtgtga ttcttcttgg tcaaagtgcg gcgttgatcc
     2761 accaccaggc cgaccgtctg gccggccgat atgtgaccct gcaccattgt gcccaggtgc
     2821 agtacggctt cgcctgcgcg ggccgtgtcc tcaatgtcaa agcggatttc gggtgattcg
     2881 attcggcccg catcgccaat ctgcccgcct tgctcagcgt agaagcatgt gcgatcagtg
     2941 atgatcgccg cacggtcgcc tgggcgaagc gcttcgcccg gcgccaaggc cttcgcggtt
     3001 ccatcaccct caaaccgcac cgcggccgtt acggttgcga ccaaccggtc agtctcatac
     3061 ttcgggcggt catccgtcgg gcccgcgtgc tcgatcacct cgcccggaac ctgcccaaag
     3121 cgcgtctcag ccagcttgcc ggccgcacgg gcccgctgcc gcgcctgctc catcagccgc
     3181 tcgtactcgc cgatatccac gcgcagcccg cgttcggccg ccatgatctc ggtgagatcg
     3241 ataggaaagc cgtacgtatc gtgcagcttg aaggcgtcct cgccgcggat cacgccgtga
     3301 tgctcttcgc gcgccagggc ggccgccttt tcgaacagct cgatgccgcg ctccaatgtg
     3361 cgtccgaacg agtcttcctc ctcgcgaatc aggtccatta cgcgctgcgg gttgctgcgc
     3421 agctccggaa aagccgcacc cattgtttcc acgatcgacg gcaccaggcg atagatgaac
     3481 ggctcgtggg tctcgaggta ttgccagccg tagcgcacgg cccgacgcag aattcgccgc
     3541 agcacgtagc cgcgcccctc gcgatccggc acggccccat cagtcagcgc gaaggtcagg
     3601 cagcgcgcat gatcagcgat gacgcgatag gcgacgtcgc gcatcacggc ctggtcttcc
     3661 acttgatcct ttgatccctt gatcccttgc tccttggctt ccaaccgtcc gccgtaggca
     3721 ggggcgccgg tcacagcctg gatcgccgca aagatcggcg cgaacacgtc cgtgtcgtaa
     3781 ttgctttttt tgccctgcag tagcgccgtg acgcgctcaa aacccatgcc ggtatcgacg
     3841 tgccgtgccg gcagcggcga cagcttgccg tccggcccgc gattgaactg gatgaacacc
     3901 aggttccaga tttccatcac ccgtgcatcg ccggcgttta ccagcgtgcg gccgctcttg
     3961 tcctcggtca ggtcgatgtg gatctcgctg cacggtccgc agggcccggt ttcgcccatc
     4021 tcccagaagt tgtccttctt gttgccggga tggatatgcg tggggtcaat gtccgtgacc
     4081 gaggcccaca ggtcgcgcgc ctccaggtcc ggctccagac cctcggcctc atcgccctgg
     4141 aagtaggtgg cgtgcaggcg ggtcttatcc aggccccaga cttcggtcag cannnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggctgct gggcacgaag tgatgcgcat
     4321 gccgctgacg gaaaaactcg atgaagtcct gccgaatttg agccgctgtg cgcgtcaatt
     4381 tgttcatcct gtttgaggca aaaccgctcc aaagcagtgc attttatgcg ggtgatcgca
     4441 aagagtcgaa cgtgcgcggg ctctctggcg gtgatgagta ggtgcttggc gggcgagggg
     4501 agtggcggcg aagccggcgg ggcggagttc gcccgcaggc gtttaaatag gccctcttga
     4561 tcaagcgatc ccttaatccc ttgatccctg cccggttggg catggtggct gcgccggtgg
     4621 cgtgctacca tttcgcgcag gcgcggagcc actttctgcc gcgcgtggcc tctgaaatca
     4681 cctgttggag accttcgaaa tcacgagacc ttcgagatta ccagattgga gaacggtatg
     4741 ccgaacagca cctacaaggt catcgagttg gtcggaagca gcgaggaatc ctgggagcag
     4801 gccgcccgca acgccgtcga gaccgcggcg cacacgctga aagatttgcg catcgctgaa
     4861 gtcacgaagt tcgacgcgcg aatcgaagac aacaaggtag ttgcctatcg cgtccggctg
     4921 aatctctcct tcaagtacct gccggagctg gcggacgacg acgacgagga cgaagagaag
     4981 aaagagcgca aggaaaaagc cccgaagcgc aagaagagat agcagagccg gggcgtgccg
     5041 tcccggaact gaaccaggag ccttaagtcg cgagttgggt aacgcaagca caaccggggc
     5101 gtgttgctcg ggaatcgagc cgtgtcgccc cggaatgagc cgagttccct cgaccggcgc
     5161 gacgcccctc aaactcctcg ctgattctcc aggcacagcg gcagttcgag gcccgcgctc
     5221 tcgagcagtt gccggtccga aagaacggtc ctggtgggcc cgtccgacac cacgcgcccg
     5281 cccgccaaga gcacggcgcg ctcgcacagc tcccaggcca ggtccagatc gtgcgtcgcg
     5341 acaagctgcg tgccgccgat cccctttagc aactcgatca accgccggcg gttgcgggga
     5401 tcgagcgccg cactcggctc gtccaagacc agtatctcag gctcgtagct gagcacggtc
     5461 gcgatggcca ccaggcgctt ctcgccggcc gagagcctct gcggaacgcg gcgttcgaag
     5521 ccgggcaggc cgacgcgccg cagcgccccc tgcacgcgct cgattacctc ttcaatcggc
     5581 aaacccaggt gcagcgggcc aaaggcgacg tcgtccagca cggtcgggca aaagagctgg
     5641 tcattcggat cttgaaacac gaagccgacc cggcgccgga ccgcggtata gttcgcggga
     5701 actacctcca gcccactgat gaacagccgc cccgccgtcg gggtcagcaa cccgttcagg
     5761 tggcgcacca gcgtggactt gcccgcgccg ttcggaccga ccagcgccac gcgctgcccg
     5821 ctttggatcg tcaggtccac cccgcacaac gcattgttcc catccgggta gcggtgcgcc
     5881 agagcctcga tgcgaatcgc gaccgcttgc gcatcccgga ctcctggcgc ctcaacaatt
     5941 cctgagggcg ggcagctcgt gttattgtcg cgcgtcacgg tcggcttcgc tcgcggcgga
     6001 cggatgcggc tgttctccaa gaacagagac gaaaacgtcc tctagcgtgg gttccacttg
     6061 ttgcacctca tggtacccga agcctgcctc ccgcagcagg gcgcccaccg cggccgcagt
     6121 ctcttgcggg tcgttcgtca cggcgtgcac gcgatcgcca aacaggctga tggttgcgcc
     6181 cggcagcttc tcccgcagca gcgcggtcgc ctcgcgcgcc cgcgaacagc gaatttcaat
     6241 aatcgttccg cgcatcagcc gcttcacttc gtccggcgtg ccgacggcaa tcagccggcc
     6301 gcggtggagc agcgccaggc gattgcagcg ttcggcttcg tcgaggtagg cggtcgaaac
     6361 gaagatggtt acgccttcac gcaccagttg atagaggatt cgccagaaat cacggcgcga
     6421 gacggggtcc accccgttgg tcggctcatc gagaaacaga atctgcggcg tgtggatcag
     6481 tgcgcaggcc aagccgagct tctgcttcat gccccccgac agccgcccgg ccaggcgctt
     6541 cttgaacgac gtcaactggc tgaagccgag caaggcttcg atcttctcat tccgcttgcg
     6601 gcgcggcacg tcgtagatgt ccgcatagaa ctggatgttc tccagcaccg tgaggtcggg
     6661 atacagcccg aagcgctggc tcatgtagcc tattttttcc ttcagcgcct cggcctcgcg
     6721 caccacgtcg tgtccggcta cccaggcctg cccggccgtg ggctcgagaa tgccggtcag
     6781 tagccgcata gcggtcgtct tcccggctcc atccggcccg accagaccgt aaatttcgcc
     6841 cggggtaacg ttcagggtga gagcgtcgac ggcgacgagt tcgccgaacg aacgcgtgag
     6901 ttgatgtgtg cgaatggcgt ccaaggggca aatgctaaaa ggcaaaccct aaagcgcaac
     6961 agtaaatggc gacagccaaa tggcagatac agcgcgctga gcgcatttgt ctcttgtcat
     7021 ttggcatcaa gctcgatttc tgcgtccgcg ggcatgcccg gcttcagctc catattcggg
     7081 ttcgcgatct cgatcttaat gcggtagacg agcttcacgc gctccttgtt cgtttgcacg
     7141 tttttcggcg tgaattccgc ctggccggag atgaacgaga cgcgcccctc gtagacctta
     7201 tcgtcgtagc tgtccgtttt gacgcgcacc ttccggccga ccttcacgcg gcccaggtcc
     7261 gtttcgttga tgtacgcccg cagccacgga tgctccaggt cgcccaccgt gaccactggc
     7321 gtgcccggcg cgacgtattc tccatcctca acgttctgcg acagcaccag ccccgacagc
     7381 ggcgaaacca atcgggcgta atcgagctgc gtctgcgcca gccggaccgc cgcggcggcc
     7441 tgctcaactt tcgcccgggc ttgatcgatt gcctcgcgcc gcggcccctg cttgaccagc
     7501 ttgcagtgct gctccgcctg ctccaccgcg gctcgggcgg cggcgatctg ctcggagcgc
     7561 ggaccttcgc gcaccagctt caggcgctcc tcgacctcat ccagccgggc gcgggccacg
     7621 tccctgcggg cccgggcgct gacgtattcc tgctcggccg ccatcgatcg ctcataaagc
     7681 ccctggatgc ggtcataagt cgcctccgct tcgcccaggt ccgcacgggc gctggccacc
     7741 gcggcttcgg ccactgcaat ctcctgcggg cgcgagccgg cttcaagctc cgccagcgct
     7801 gcccgggcgc gatccagggc cgcctccgcc tccgcgatct cttccggccg cgcgcccgcc
     7861 tcgagctcgg cgagcaccgc cttggcggac gcgagttccg cgttgcgcat cgccacctgg
     7921 ctttcgagct ccgcggtgtc caacagcgcc accaagtcgc cgcgctgaat gagttggccc
     7981 tcggaggcga cgcgcttgtc gagccggccg gcgattttga aactgacctc cgcatccgtg
     8041 acttcgatgt tgccggatac gtaaatcaca ttcggttcgg cgcccccgcg gttgtggaaa
     8101 aaagccccca gcgaggcggc ggcgatgctc agcaagaaga gcgtaagggc cgctttcatg
     8161 cgggtcggca ctgttttgaa tcctgttcca atcgtgaagc tggccggacg ggactcacgg
     8221 aacggcagga tacgcggagt cggtcaggct gacaatgcct tccaatgcag aacattgaga
     8281 gggatcgagg gatcaaggga tcgagggatc aagaagtgct cgggcgcccc ttcagcgagc
     8341 ggagcggcag tgatgccgag cagagccggg gcgtgccgcc ccggaatgcc gccaacaccc
     8401 tcccacccgg gcagtcgccc gccccgtgaa ccgcgcctgc ctgacccgcg tcaaccccat
     8461 ccgcgcccgc ctgacccgcg cggacccgat ccgcgcccgc cgactgaatc cgagcccgaa
     8521 gcgcaagcga gggcaaccgg tcccccaatc cgagtccgta ggaaccgagc ccgaagcgag
     8581 ggcaagcgcc aatgcaacaa cgatccgggc ccgtaatgcc ggaccgggtc ggcgacatac
     8641 gtagcgtcgg ccccccgtgg ccgacgtcgc gctcatcagc atggcgcttt gcgcagcggc
     8701 gacgctagtt ggttggcatc tgtcggggct ggcagtcaga tttcatctgc cggcgacttc
     8761 gcgatttgcg cgcgtgtcaa cgttcttggt cgcgatgagc gcggccctga tagcgctggc
     8821 cgcaatgctc gacctgtccg gcgcggcgcg cctggctggc tgggcgcgct catatggaat
     8881 cactcaggtc ccagttgcgt tgggcgtgct aggcatatgg ctgacggtaa ggcagctcag
     8941 gaggctgttg cgtgaggccc tagaagtcgg cagagatctc cctgactgcg ccggacaatg
     9001 aagccgtgaa cggcgcggta tccaggcatt ggcagcccgt cagtcaccat gcgcggcacc
     9061 agacggccgt tcgggtgtga aacgccggct aatccgcagg cgcaatacat atttcagtta
     9121 acaaccaaca tatagcagcg tggtgtgcgc gggagcagtg gcacttccgg ctcgccgggg
     9181 tatcagcatg gcgtccccgg attacctccg ggttaccttt gcacagcgct tgcgacgcct
     9241 ccagccgcca tggccttcac atcagccgta ctgagcgact tgcccccgat cccccagtct
     9301 ccgctcttca cttcctcgac aattaccgtc gtgacctcgc gcaggttctc cccctcgatg
     9361 gataccatgg cgtcggttaa cttcgcaatc atctgctgct tctgcttcgg cgagaaaacc
     9421 ccttcgataa gtttcacctc gataagcggc atgatcgaat ctccatagcg aaaagctctg
     9481 catacgttgc tcgccaacct gttgagctga tgatagctcg gacaatggcc cgagtctgcg
     9541 gcgcgccgac agggaggcgg gcaatgcggt gagcggttaa tccggagaca tcattcccgt
     9601 tgacaacgca gatatagcag cgtaatgtgc gcgcgggaat gtgaaagcgg atgagcggct
     9661 gaaatgagta gggtgtcacc ggaattgcgg ctaactggcc acaaaggggc agcgggggaa
     9721 gtccgggagc cagtgccgag aatgaacaaa tgagtgaggg tgccgtcgga ctgatcgtct
     9781 tgtgtgccag cgctgtggtt agtgtctgtg gtctgtcacg ctctgataag gtccgcaaaa
     9841 gttgcgatcc cggtcgccgc cacgatagcg gccgctttgt ttcacgtcct tgcatcctta
     9901 caccagggat atcttgaccc gttcttcccc atagcctgtt tcgtgagttg ggtccttgca
     9961 gttgcgatcg ccggggtggt cggtattccg ttctacctca tgcggagaac tgctacgaca
    10021 ggccactgtc agaaatgaca gctcgacact gcttgaaagc agtggcaccc cggccgtaac
    10081 tctggtctag cgctagcgcg ccagccggcg ggcctggctg gcaaccagaa ccagcaggta
    10141 cgttgcgccc gcgacgagaa cagtcgtcgc gccgggttgc aggccgaggc tgtagcccag
    10201 ggccaggccg agcgtgacga agaccatgct cagcagggcc gcgaccgcca ttattcgcca
    10261 gaagatgcgc gtaaactggc cggccacggc caccggcagc gtaaacaggg cgatcaccag
    10321 gatgattccg accacgctca cgagcagcac gaccgtcagg gccaccaggc acagcagcag
    10381 caggtaatag aactcgacgt tcaacccacg caggcgggcg tattcctcgt cgaagcacac
    10441 ggccagaaac tgcttataga acagcagcgc cacggcgatt atgagcgcgt cgagcacggc
    10501 caggaaccaa acgtcgttcg gcgagacgat caagatgctg ccgaagaggt agctcatcag
    10561 gtcctgcgaa tagccgggcg tgagatagac gaacagtacc ccgacggcca tgccgatggc
    10621 ccagagggcg ctgatgatgg tgtcctcgcg ctgccgggcg cgcaggctga caaagccgat
    10681 gatcatcgcg gccagcaggg cggcgactac ggcaccgtga agcgggtcaa gcggctgcca
    10741 gccgtaaatt gggttgtaga cttgccagtg gtggacgcgc tccaggtaat aggccgcgcc
    10801 caggccgccg aagacgcagt gcgcgaggcc gccggcgatg taggtgatgc ggcgcacgac
    10861 gacgtaggtt ccgacaatgc cgcaagcgat gctggcgaga atgccggtga ccagcgcgta
    10921 taccaggaaa acgctgtcgg ggtcgcgcag agcatggaag aaatcgagca tggcggcctc
    10981 actcgtgcgt gccgtgggaa tgttggacga agcgcaccgg gccgccatac agggcgctga
    11041 tggtgcggcc gttcagttcc ctggtggcgt gaacgtcaac gcgccgcgcg acgcagatga
    11101 cagtgcgcac gtgctgagac acgaagccga catcgtgcga gacgagcagg atggtcatgc
    11161 gtttgttgag cccgtgcagc agttcgagga aatcgccctg gacggcgatg tcgaggttgg
    11221 cggtcggttc gtccagcagc agcaattcgg gctctgaagc cagggcgcgg gcgataagta
    11281 cgcgctgccg ctgaccgccc gagagcgccg gcagcgggcg gcgggcatag tcggccaggc
    11341 cgacttcggc cagggcctgc gcggctgcgc ggcggtccgc ccggccggcg gggccgaatg
    11401 tggcggaaat tccgaggcgt cccatgagca ctacgtccag cgcggtgacc gggaactgcg
    11461 ggtcaaattg tgcgtgctgc ggtacgtagc cgatgcgctg gcgcacctcc agcggcggcc
    11521 ggccgaagat gcggatggtt ccgctctgcg gctggagcag tccgagcatt aatttaagga
    11581 gcgtggtttt gccgccgccg ttggggccga cgatggagac gaattcgcgg gcggcgattg
    11641 agaaggttac gtcttcgagg accggtgcgc cctggtaggc gaaggtcagg cgctcgaccg
    11701 cgatgacggt ttcgggcggc gcgctcggcg acgggttgtt attggccgga gcggtcacgg
    11761 gtgctgaggc tccgcggggg cggtggcggg agacgagggc atgcccgcgc cgctgcgcgg
    11821 cttgggcatg gcacccgttg gcggttcgtt cgccgttggc ggtggttcgg gggccgattc
    11881 agtgggctcg gatttcgcgg gttcgggttt ttggggatcg ggttttgaca gcgcctggcg
    11941 gacctgctcg gccatggttt ccatgctcgt catgtaatcg ggcgcgaggt cgtccagcgg
    12001 gacaaccgct ccgccgattt gccgcgcgat tatttccgcg ctacgcgtcg agaactgcgg
    12061 ctggacgaat atgacgcgca cgttttcctg ccgggcgcgg cgaatcagtt cggcgaggtc
    12121 cttgggggag ggctcttttc cgccgatttc gaccgggacc tgatgcagtc cgtaggcgtc
    12181 cgcaaaataa ccgaaggccg ggtgatacac gaacagtggc tggccgcgca cgggcgcgag
    12241 agcagcggcg atgcgggcgt cgagcgcgtc cagctccgca gtaaaagccg ccaggttgcg
    12301 gcggtactcg tcggcgttgg ccggatcgag tcggatcagg gcatccgcaa tagtctgggc
    12361 ctggattttg accagccgcg ggttcagcca gatgtgcgga tcggcgcctt cctcgtcgtg
    12421 gtgcgccgga tgttcgtgat gcgcctccga accgtgttcg tgtgcatggt gcgccgcggc
    12481 gtcgtgttcg tgcccatggt gcgcggcgtc gtgctcgtgc tcgtgttcgt gttcctccat
    12541 gggccgcaag ggcacgccct ggcgcgtgtc cacgatttcc aagtgcgtga acgcgccgcg
    12601 caagcgcggc agcaggccgt cctcgaatgg cataccaacg cggaaaaaaa cggccgcctt
    12661 ggcgagcgag gagatttggg ccggagttgg ctcgaagctg tgcgggtttt ggccgggccc
    12721 gaccaggacg tcaacctgca catgatcgcc gccgacgcgt tggacgaagt acgcctgcgg
    12781 cggaatgctg acgaagacgc ccagacgctg accgccttcc ggctgcggcg cggcggccgg
    12841 gttcgtctgt ggcgtgccgc tgggcggcgc ctggcggcag gcggtcagaa cgagtaccgg
    12901 cgcggccagt gccagcagaa aggcggcgag acgccgattg gcggcggagg cgaaattcgc
    12961 ccgcagtcgg ttgtttcggt tcatccaggg ctccgggagt gcggaacggc cggcgcacgg
    13021 cacgccttag ccttattgat attacgttat cagttagggt gggttcgtca agttttcacg
    13081 gttatcaaac ttgcaggccg ggcgagcccc tacgccgggg cgttcgccac tggcgtggcg
    13141 gcgtggtaga ttcggcgcct gatgacggag gacgcgcagt cgatgaggca gcttgagcat
    13201 cgccggcctt catggctgct cgacggcgtg atcgtgctgc tgatcttggc cgcgtggctg
    13261 ccggtggcgt ggtacctcac gggcggcctg gggatcgtct ggtacgacac gtttcgcgac
    13321 atggcctggg ccgagaacat gcgcgccggg cggatttggg ctgacccggt cgtagcgggg
    13381 cagacctggt ggtacgctcc gggcgggccg ctgctggcgg ccggtgtggc acgtttgacc
    13441 gggtggccgg tggcagaggt ctatgccgcc agcccggcgt ggtgcaatgg gtggattccg
    13501 gcggcgctgt atgtgctggc gcgcgtggct tgggggcgcg gcacggcttt gctggcgctg
    13561 gcgacggtgc tgctgggctc ttactggtgg tacacgcacg tcgcggcgcc gattcccggc
    13621 attcaggcgg tcacgctgat gttgctggtg ctgctcggct ggcacctaag cgtgacggcc
    13681 ggcagggcgt acacgtgggc ggcgatttcg ggcgtactac tggcgctagc cacctggtat
    13741 cacccgatgt gcggtattct cccagcattt acgatcgcgt tgcaggtgct ctacgacgca
    13801 ctgcgcccgc gcaagtgccg ccagcggctg ctggggcgca tgctggtcgt ggcgggcac
//
LOCUS       DAOSYS010000076        13788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3755_length_13788_cov_5.527124, whole genome shotgun sequence.
ACCESSION   DAOSYS010000076 DAOSYS010000000
VERSION     DAOSYS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13788
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_13788_cov_5.527124"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="PLU99_06440"
     CDS             <1..1144
                     /locus_tag="PLU99_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16732.1"
                     /translation="SADMEFGMDEADERSEPAETKRAGLMTVVLKAGALLAGLGILAW
                     CAERLIEMQGATNVSASEVLGSLAIMFAGLGLALLVWGVAEAVRRLDEIRDLLDRSEQ
                     ASSPAAVGRAGVHADGRVFEDLKTGVDELISLTREVRDVSLLSEAERARRLQVQGQAL
                     LAVLQQEIPALLQNHQWVEARQRVQRARERFPSLSEWDQLEQQIESMRGSVEARDVEN
                     AARQINDLAALGAWERAGGLVRELAERHPDSPRVQELLRRVSIQREKADAEQRARLMA
                     QAQEAVNQREWNKALVLANDLLHRYPRSPEADAVRLQLATLTENAEIQTRQQMESEFR
                     EQMKQRQFANALRLANELMERYPKSPQADVLRSQLPRLQKLAAEQK"
     gene            complement(1174..1830)
                     /locus_tag="PLU99_06445"
     CDS             complement(1174..1830)
                     /locus_tag="PLU99_06445"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOO16733.1"
                     /translation="MAFQNEERAAERARMVTEQLAGRGIQSAAVLEAMRRIPRELFLP
                     PGAQECAYVDAAVAIECGQTISQPYVVARMTELLELTPEARVLEIGTGSGYQTAILAS
                     LAKRVYTIEWHLPLMVQAAERLKQLGLNNVTFRCADGSRGWPEQAPFDGILAAAGAPA
                     VPPSLLSQLAPGGRLVLPVGGEHDQTLVRVRQTDTAPQTEEFFKCRFVKLIGAEGWQQ
                     "
     gene            1959..3524
                     /gene="rmuC"
                     /locus_tag="PLU99_06450"
     CDS             1959..3524
                     /gene="rmuC"
                     /locus_tag="PLU99_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOO16734.1"
                     /translation="METALAAIMALLMFAGGVAAGCLLMRGAARQARAAAQAEVAAER
                     ATLLERLATREAEARAGRSELESLAAEVRQLQEQMRDAAVRCSAAEEKNARIPELEAA
                     LKERDERIAALLDETRTFAAQVAELGTKLDEERKAGEAKLALLNEAQQKLSDAFKALA
                     AEALKSNNQSFLELARTKLEKFQGEARSELELRQKAIGELVRPLKDSLEKVDTRIQEL
                     EKARTGAYAGLTEQLKSLSLTQSQLQNQTANLVKALRAPQVRGRWGEIQLKRVVEMAG
                     MVEYCDFKQQQTAAGDSGRLRPDLIVRLPGRKNVVVDSKAPLSAYLEALEAEDEDQRL
                     ARLREHAAQVRKHVANLSSKAYWDQFQPAPEFVVLFLPGESFFSAALEQDPALIEAAV
                     AKQVILATPITLIALLRAVAYGWSQERLAENAQQISRLGRELHDRLRVLAGHFADIGG
                     ALSRAVESYNRMLGSLESRVLVTARRFKDLGAASGADIEVLEAVDRQTRTPISTGGSE
                     DTCTPELPLLPGD"
     gene            3547..5208
                     /locus_tag="PLU99_06455"
     CDS             3547..5208
                     /locus_tag="PLU99_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16735.1"
                     /translation="MPPTVRIPLLGSLVLFACALGVRASAEPAPPNSPVESPCAATTN
                     PRPHVAVAGFELAAGADERDAWLPLAFEEMLAWRLRRVSGLLTVPVVRVHQTRRELQE
                     EGEPPPSWPRVVAAMGPKYMLTGRCSGPPYEATLHLAVLEFSSAAGEASSKPSADTRV
                     WQEVDLPPGKLFDVLDAATRWTLDQFEIREVPQSVQDMIFGPPCRTPSAVEYYARAVT
                     AARAGDLRSAYDYAARSLGYDERFRPTLALMVQLEVQSGKVGRNSAMRLLRQWAELTR
                     ICADPVDRAAAELGFGAVLHMDGTFDAAYQRYENALAMAYADDSPYGQMLAANSLADL
                     FSSRRLPQQPVLTDAQLRTLAQQDLRAAVAWLEIALDLASGLGDLATEAPCANKLALT
                     YERLGEWEAAIAMHQRSLAAAQRIGARQSEAAAWLCLAQGYQRASKPAEALAAAQRAL
                     PLANPALQPMVRIVIGAAYRILKQPEAALAEYEQAYRELRETDDLGSQLACVTGVADL
                     RMQLGRRAEAISALQEAIEIAHALSATQEEASLREQLANWNQNVP"
     gene            6675..7673
                     /locus_tag="PLU99_06460"
     CDS             6675..7673
                     /locus_tag="PLU99_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOO16736.1"
                     /translation="MRPNAVRLAWLCLLGFVGIVGCESPGVRFLNFITLQKNPLVLAL
                     VAENRATNDDPLQALNPFGPYSALQHAMSRDLQRTVALDLCFPFQLQPCLETGLYHAA
                     VVSPIQYARLSEAQRFPVIAVPAGEGEPIVQPALLVVPADSLIQAVEDLCGETVAFGP
                     APDSRTHYAALDLLAAHGLQKADLRLELLPIPGSLKHLPNAREVVSCVLSGGAAAGFV
                     DEGEWQKLPETSNADGAIARDKLRVIARTAALPNQLVVASPRLDPQTLERMQSFFIHA
                     GETHPEALQPLRIERFELPSDELLATCRELAVRESRPAAVNGGAEALSPAADSPAQ"
     gene            complement(7689..8963)
                     /locus_tag="PLU99_06465"
     CDS             complement(7689..8963)
                     /locus_tag="PLU99_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO16737.1"
                     /translation="MNMRRGQASPRAVLLGAMLFIVAGFLVFFASPWILPVRIVEGPL
                     VQAAGPDEVTLVWYTSRPVKPGDCLVTLGAPPDSRDYPAEANDTRQRVRITGLTAGTA
                     HPYRIQLGRRTLAEATLRTNRAAGEPFAFIAFGDSGLGTREQYLLAQRMRSLEFSPDF
                     LLHTGDVVYKRGERNNYEERFFAPYRDLLSQITFWPTLGNHDVLTDQGDPYLGIFELP
                     HNGPADLQPETNYWFDYGSARIVVLDSTLPEATLAEKVAPWLKQTLAAAPATWKFVVF
                     HHPPYTCGAHPSDERIQRAIVPAMEAAGVDLVFCGHDHLYERTHPLRGGQVVPDGTGI
                     TYIVTGAGGARLYEAPPPDQRPAYVAELDNSQHSFTYVQVDGLELRVRQIGLNGATID
                     DWRWSKPRPPMAQSPQFDSPSSQPAEQPANQP"
     gene            complement(8960..10123)
                     /locus_tag="PLU99_06470"
     CDS             complement(8960..10123)
                     /locus_tag="PLU99_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16738.1"
                     /translation="MILLTTPRGKRFGRATAAVLLSAIAVVASAAGPASVPPSTQPAL
                     SHLADQIARGTPDEAAAAADEFVRQIVEPVAAVLKNLEQRSPAEQRRVLQALQRVHAA
                     LRIRLYQANLPPEDAQLFDQVAAGNRDLLERLFDDDPRRRLAALYQIPLEPGSAAGVL
                     VTSRLFDWDALVVEAALKIAARLGDDPATMRGLERFVDGASQAVCGTTGALPAEEAAA
                     WADFSNRAICLLGNSKARSSVPVIVEAIRCFGRPPYRTLFRVAETLDALGKIGDERAV
                     PLLLECTTDTEQHAVRSLGAGSLLRVTVGDAALLALARIYGLPPDELGFVSAPGDHPV
                     TGFLDERTRRNALRLFALWYKENGEKPPQERTPLIPQATPTTEPAAHAPAEQP"
     gene            10399..11622
                     /locus_tag="PLU99_06475"
     CDS             10399..11622
                     /locus_tag="PLU99_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16739.1"
                     /translation="MRKNNRVVPWFTGSGSILTLAGAVMLMLAWGCSKSADTPPEKPP
                     AAPAASPEAGAPQTAVETPAPQPAAAPATPPAAGNVSPPAAAPTPPPAANVPPPAAPT
                     AGNAPGASAVRPDSEEARRLIEEKLAQLQSAGAKPAPGQAVTPPPSAGAPSVPASGAQ
                     AAPQPLPPPPAPAAGEQKPGRTPILDGESLDMAPPPPDQPQPRAVYDRELIVADPIWR
                     GESAKFLFNVRNEGEGVLNMKMKACCGLSINGVRNGWPGWSLRPGESETFEMTFGPSA
                     RGDFTRYSPLHTNDPQNPKINFTLKGRVLVAFNVEPHVVNLGVVQPDSGPITKTLTIT
                     RGDGGPLQPKLNPPKDPRVKAELKEVEPGERYTLEVTVTPPLPHDYRSSLVLDTGVSQ
                     APRETILIVTQHEEH"
     gene            complement(11656..>13788)
                     /locus_tag="PLU99_06480"
     CDS             complement(11656..>13788)
                     /locus_tag="PLU99_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO16740.1"
                     /translation="FLLVTAVPLALCVGCSKKPAATDKLAGRAADCNVLLITMDTTRA
                     DHLGCYGHPGNLTPVLDNLARTGVRFSNAFTHAPITLTAHASLMTGTLPPEHGVRDNT
                     RFALGPELPTLAEIFNQHGYRTGAFVACAILQARYGLNRGFENYRDDIPISPSGEILR
                     SHSAHSMSNQALAWLEEVRDQRFFCWVHYFDPHDPYTPPEEYAARAGGPYAGEVAFMD
                     ANIGRLIDWLQINNLRERTLVIAVADHGEGLGDHGYDLHTLLLNVELMHVPLIMSLPG
                     RLPQNVVCADLVAISDIMPTVLELVGWPVPQTVSGVSGLSALAGRPPASRAIYGETEY
                     PFYSFGWSALYSITTPQWVYIRAPRNELYDRQADPRQMSNVAAARPEVVTQLESELQA
                     FEHAVQRRAGVSVKPDSVQERALRSLGYVGDTPSPSSTAPALKNPRDMIAVERDYRTA
                     QELAPHAPAEAIKLLEPAAEQSPESFAILHLLGNCYGLVNRLDESQLLLTQALQLYPE
                     SSQAALDLVLTLASRGRYAHGIELCQQVLEREPNHGQAEQMLSALLDNLRRQQEGIAR
                     AEAYCAWNEDASDPEVFCNWADLLAAGGREREALKVLKEAHAAKPADPRLCAALAWYQ
                     STTHDENLRDGPRALELAEFAVRSGDPDPRSAAALAAAYAETGRFEEAVRIARQIRAD
                     FPRDSRVPGNLDFQIRQYEARRPYRVLP"
BASE COUNT         2384 a         4217 c         4766 g         2421 t
ORIGIN      
        1 ttcggcggat atggagttcg gcatggacga agcggatgag aggagcgagc cggcggagac
       61 gaagcgcgcc gggctaatga cggtggtgct gaaggcgggg gctttgctag cgggccttgg
      121 catcctggcg tggtgtgcgg agcgcctgat cgagatgcaa ggcgccacga acgtctcggc
      181 cagcgaggtt ctgggcagtc tcgccatcat gttcgcaggg ctcgggctgg ctctgctggt
      241 gtggggcgtg gcggaggcgg tgcgccggct ggacgagatc cgcgacctgc tggacagatc
      301 ggagcaggcg tcgtcgccgg ccgcggtggg gcgagctggg gtacacgcgg atggccgcgt
      361 gttcgaggac ctgaagacgg gcgtcgacga gctgatcagc ctgacgcgcg aggtgcgcga
      421 cgtatcgctg ctgagtgagg ccgagcgggc gcggcgcttg caggtgcagg gccaggcgct
      481 tctggccgtt ctgcagcaag agatacccgc actgctgcag aaccatcagt gggttgaggc
      541 gcggcagcgg gtccagcggg cgcgcgaacg tttcccaagt ctgagcgaat gggaccagtt
      601 agagcagcag attgagagca tgcgcggcag cgttgaggcc cgcgacgtgg agaacgccgc
      661 gcggcagatc aacgacttgg ccgcactggg ggcctgggag cgggccggcg gtttggtgcg
      721 cgaactggcc gagcggcacc ccgattcgcc gcgggtgcag gagctcttgc ggcgggtatc
      781 gatacagcgg gagaaggccg acgccgagca gcgggcgcgc ctgatggcgc aggctcagga
      841 agccgtgaac cagcgtgagt ggaacaaggc gctggtgctg gccaacgatt tgctgcaccg
      901 ctacccgcgc tcgccggagg ccgatgcggt gcgcctgcaa cttgcgacgc tcacagagaa
      961 cgccgaaata cagacgcggc agcaaatgga aagcgagttc cgcgagcaga tgaagcagcg
     1021 gcagttcgcc aacgccctac ggctggccaa cgagctgatg gagcgctatc ccaaatcgcc
     1081 gcaggccgac gtgctgcgca gccagttgcc gcggctgcaa aagcttgcgg ccgagcagaa
     1141 gtaaccgggc cggtttgctt gccggcctat ggatcattgc tgccagcctt ccgccccaat
     1201 cagctttacg aagcggcact tgaagaattc ttccgtctgc ggcgcggtat cggtttgccg
     1261 cacgcgcacc agcgtctggt cgtgctcgcc acccaccggc aacacgagtc gtccgcccgg
     1321 cgcgagttgg ctgagcagcg aaggcgggac ggccggggcg ccggcggctg ccaagatgcc
     1381 gtcgaagggc gcctgctcgg gccagccccg cgagccgtcg gcgcagcgaa acgtcacgtt
     1441 gtttaatcct agttgcttca ggcgttcggc ggcctgaacc atcaggggca ggtgccattc
     1501 gatggtgtaa acccgcttcg ccagggaggc gagaatggcc gtctgatagc cggagccggt
     1561 gccgatttcg agcacgcgcg cctcgggcgt cagttccagc agttcggtca tgcgggccac
     1621 cacgtacggc tgcgaaatgg tctgcccgca ctctatggcg acggcggcat ccacgtacgc
     1681 gcattcctgc gcgcccggcg gcaggaacag ctcgcgcggt atgcgccgca tcgcttcgag
     1741 cacggccgcc gactgaatgc cgcggccggc gagttgctcg gtcaccatac gcgcccgttc
     1801 tgcagcgcgt tcttcgtttt ggaacgccat ggcgatagtt tatcgtagga tggttgactt
     1861 gcccgttccg gccgcagtcg gcccggcgtt tttgctaccc cagcctgtca ctgcggctta
     1921 agatgtccgc gcagaggcgt ttttcaggag acgggaacat ggaaacggcg cttgctgcca
     1981 tcatggcact actcatgttc gccggcggtg tcgccgcggg atgcctgttg atgcgtggtg
     2041 cggcccggca ggcgcgggcg gcggcgcagg ccgaagtcgc ggcggaacgt gcgacgctac
     2101 tcgagcgcct ggcgacacgc gaggctgagg cgcgtgccgg ccgcagcgag ttggaatccc
     2161 tcgcggcgga ggtccggcag cttcaggagc agatgcgcga cgcggccgtg cgttgctcag
     2221 cggccgagga gaagaatgcc cgcattccgg agcttgaagc cgcgctgaaa gagcgggacg
     2281 agcgcatcgc ggcgcttttg gacgaaacgc gtaccttcgc ggcccaagtg gcggagttgg
     2341 ggacgaagct ggacgaagag cgcaaggccg gcgaggcgaa gctggcgcta ctgaacgagg
     2401 cgcagcagaa gctgtcggac gcgttcaagg ccctggcggc cgaggctttg aagagcaaca
     2461 accagtcctt tctcgaactg gctcgcacga agctggagaa attccaggga gaggccagaa
     2521 gcgagttgga actgcgtcag aaagcgatcg gcgagttggt cagaccgctg aaggactcgc
     2581 tggaaaaagt tgatacgcgg atacaggaac tggagaaggc ccgcaccggc gcctatgccg
     2641 gcttgacgga gcagctcaaa tccctgtctt tgacacagag ccaattgcag aaccagacgg
     2701 cgaacctggt gaaggccttg cgcgccccgc aagtccgcgg gcgctggggg gaaatccagc
     2761 tcaagcgcgt ggttgagatg gcgggcatgg tggagtactg cgacttcaaa cagcagcaga
     2821 ccgccgccgg cgatagtgga cgcctcagac ccgacctgat cgtgcgccta cccggccgaa
     2881 agaacgtcgt ggttgattcc aaggcgccgc tgtcggccta cctggaggcc ctcgaggccg
     2941 aggacgaaga ccagcgtctt gcccggttgc gcgaacacgc cgcgcaggtg cgcaagcacg
     3001 tggccaacct gagcagcaag gcttactggg atcaattcca gccggccccg gaattcgtag
     3061 tgctattcct gcccggcgaa agcttcttta gcgccgcctt ggagcaggac cccgccttga
     3121 ttgaagccgc cgtggccaaa caggtgattc tggcgactcc cattacgctg atcgccttgc
     3181 tgcgagccgt ggcctacggc tggagccagg aacggctggc tgagaacgcg cagcagatca
     3241 gccggctcgg cagagagttg cacgaccgac tccgcgttct ggctgggcat ttcgctgaca
     3301 ttggtggcgc ccttagtcga gccgttgaat cgtacaaccg catgctcggt tcgctcgaaa
     3361 gccgcgtgct tgtcactgcg cggcgattca aggacctggg tgccgctagt ggggcggata
     3421 tcgaggtact cgaagcggtc gaccggcaga cgcggacgcc gatttcgacg ggtgggtcgg
     3481 aggacacctg cacccccgag ttaccgctgc tgcccggtga ttgaacagac cccaaacgga
     3541 ggagagatgc cgccgaccgt gcgaataccg ctgttaggct ccttggtgct cttcgcgtgt
     3601 gctttggggg ttagggcaag cgcggaaccc gcaccgccaa attcacccgt cgagagcccg
     3661 tgcgccgcaa cgaccaaccc acgtccgcac gtcgctgtgg ccggtttcga gttggcggcc
     3721 ggcgccgatg aacgggacgc atggctgccg ctggcctttg aggagatgct cgcctggcgt
     3781 ctgcggcgcg tttccgggct gctgacggtg cccgtggtgc gcgtgcacca gacacgccgc
     3841 gagcttcagg aagaagggga gccgccgccg tcttggccgc gagtcgtagc ggcaatgggg
     3901 ccgaaataca tgctcacggg gcgttgcagc gggccgccgt acgaggcgac tctgcacctg
     3961 gccgttttgg agttcagcag cgcggcgggc gaggcttcgt cgaaaccgtc ggccgacaca
     4021 cgcgtatggc aggaagttga tctgccgcct ggcaagctgt tcgacgtcct ggacgccgcg
     4081 actcgttgga cgctggacca atttgagatt cgcgaggtcc cacaatccgt gcaggacatg
     4141 atcttcgggc cgccgtgtcg cacgcctagc gcggtggaat attacgcccg ggcggtcacc
     4201 gcggcgcgcg ccggcgacct gcgcagcgcg tacgactatg ccgcgcgcag cctgggttac
     4261 gatgagcgtt ttcgcccaac gctggcattg atggttcaac tcgaggttca atccggcaaa
     4321 gtggggcgaa actcggccat gcggctgctg cgacaatggg ctgagttaac ccgaatctgt
     4381 gccgatccgg tcgatcgggc cgcggccgaa ctgggattcg gcgccgtgct gcacatggac
     4441 gggacgttcg atgcggctta ccagcgctat gagaacgcgt tggcgatggc ctatgcggac
     4501 gattcgccct acgggcaaat gttggcggcg aacagtctgg cggatttgtt ctcatcgcgg
     4561 cgtttgcccc agcagccggt gctgaccgac gcccaattgc ggacattggc gcagcaagat
     4621 cttcgcgctg ccgtggcttg gctggagatc gccttggatc tggcgagcgg cttgggagac
     4681 ttagccaccg aagcgccatg cgcgaacaaa ttggcgctca cctacgagag gctgggcgaa
     4741 tgggaggcgg cgattgcgat gcaccagcgc tcgttggccg ctgcgcagcg gatcggtgcg
     4801 cggcagagcg aggccgccgc ctggctctgc ctggcgcaag gctaccagcg tgcgtctaag
     4861 ccggccgagg ctttggccgc cgctcagcgc gcgctgccgc tagcgaatcc ggctttgcaa
     4921 ccgatggtgc gaattgtgat tggcgcggcg taccgaattt taaagcagcc cgaagcggct
     4981 ttggcagagt acgagcaggc ttatcgcgag ctgcgcgaaa cggatgacct cggcagccaa
     5041 ctggcatgtg tgacaggcgt agctgacctg cgaatgcaat taggccgtcg agctgaggcg
     5101 atcagcgctc ttcaggaggc catcgagatc gcccatgcgt tgtctgcgac gcaggaagaa
     5161 gcgagcttgc gcgagcaact ggccaattgg aaccagaacg ttccttagct tcggccaacc
     5221 ttggatcgag ccatcgggca gccttggatc agcgaacgtt agagtgcgaa cgagataacc
     5281 gtggattcag ccgcgcatgc ggccgggcgt gaaggggggc ggtttcgcgg gttccaggcg
     5341 ggtgccattg ggtggggcgg agggctgtgg cggaggcgga tcggaaccgg ttccggccag
     5401 aaacggccgg acttgcgcac atgtgtccga tttgtaactt atttattgtc aatcacttac
     5461 aaaaaatcag cccatccacg caaaacccgc cccccttgtt ggacaccccc ctggcgatct
     5521 gatatagtgc gcccgtgggt cttgtacccg cggcaagcac ggatattacg gctgttctag
     5581 ccgtggcgca cttccgagat cggccggttt ccggttcgat ctcggaagtt tttgttaggc
     5641 cgactttgtc gagcaagcgc gagggcgcgc ggtgattcag tcaagcacat cggggcgcgt
     5701 ggcggcactc gaaacgcgcg tggtggccag ttttcctgta tgccgcgagc acgtggcgct
     5761 tgagtttgtg gcggatcact tcccgccgtc gactccagga caattcttac aggtgcaatg
     5821 tgctggttgc gcgagcggct cgccaagctc agcgcgctgg tcgcaaggac cggctccggc
     5881 gcgttccgcg gcggatcagc cattcctgcg acgcccgttc agcatcgcgg atcgatggac
     5941 cgacgccgct ggtggcgtcc atctcgcggt catttcgcgg acggtggggc gtggaacggc
     6001 gtggctcgag agcttgcggc ccggcgatac gctcaacgtc accggtccct tagggcgagg
     6061 tttccgaatt ccggagcagg acgtgccatt ggtgttgatt ggcggaggcg tcgggattcc
     6121 gccaatgctc tacttggccc ggcgcctgca cgaactcggg cgtcgcgagg tctgcataat
     6181 tttcggagcc accacgcgtg atctgctgcc agtagcactg cgtagcgagc ctccgcggga
     6241 tggcggtgca gcgccttgcg tcgttttgcc cggcggggct gagtttttgg cgatggttac
     6301 atccgacgac ggctcggtgg gcatgcgcgg agtcgttacc gacgcgttgg cggcgtggca
     6361 gagtcggcgg ggcggcgcct ctgccaaagt tcgcgggcag gtctttgcgt gtggcccgga
     6421 gggaatgttg aaggcggttg ccgcggtgac tcgcagactg ggcctggcat gtcaggtttg
     6481 catcgagcga accatgggat gcggcttggg cacgtgcctt tcctgcgttg tccggcggcg
     6541 cgatccgggc cgacccagcg gttggtcgtg ggcactggca tgcagcgatg ggccggtatt
     6601 tgaccgggat gagctgctcg attacgactt accagcgacc gcataatggg ggtggatcgc
     6661 gtggagaagt cctgatgcga ccgaacgccg ttcgtctcgc ctggttgtgc cttttggggt
     6721 tcgtggggat cgtcggctgt gagagtccgg gtgttcggtt cctgaacttc atcaccttgc
     6781 agaagaaccc gcttgtattg gcgttggtgg cggaaaaccg cgcgaccaac gacgacccgc
     6841 tccaagcgct caacccgttc gggccatatt cggcactgca gcacgcgatg agccgcgacc
     6901 tgcagcgaac agtggcgctg gacttatgtt ttccgttcca actccagccg tgcctggaaa
     6961 ccggcctgta ccacgcggcg gtcgtctcgc cgattcagta cgcgcggctg tccgaggcgc
     7021 agcgctttcc ggtaattgcg gttccggcgg gcgaaggcga gccgattgtg cagccggcat
     7081 tattggtggt tccggcggat tcgctgatcc aggcggtcga ggacttgtgc ggggaaacgg
     7141 tcgcattcgg accggcgccg gattcgcgca cgcactacgc ggctctcgac ctgctcgcgg
     7201 cgcacgggct gcaaaaagcc gacttgcgcc tcgagctgct gccgattccg ggttcgctga
     7261 agcacctgcc caacgcgcgc gaggttgtct cgtgcgtact cagcggcggc gcggccgccg
     7321 ggtttgtgga cgagggcgag tggcagaagc tgcctgagac ttccaacgcg gatggcgcca
     7381 tcgcccgcga caagctgcgg gtaatcgcgc ggaccgcggc cctgccgaat caactggtgg
     7441 ttgcctcacc gcgactcgat ccgcagactc tggagcgtat gcagagcttc tttatccacg
     7501 ccggcgaaac tcatcctgag gcattgcagc cgctacgcat tgaacgattc gagctgccct
     7561 ctgatgagct actggcgacc tgccgtgaat tggctgtaag ggaatcgcgt ccggcggctg
     7621 taaatggcgg cgcggaggcg ctgtcgcccg cggctgactc tccggctcag tagtcagcat
     7681 ttacgggttt atggctgatt tgccggctgt tctgctggct gcgaagaagg cgaatcaaac
     7741 tgcggcgatt gtgccattgg cgggcggggt ttggaccagc gccaatcatc aatggtcgca
     7801 ccattcaggc ctatctgccg cacccgcaac tcgagcccgt caacctgcac gtacgtgaag
     7861 ctgtgctgcg agttatccag ctcggccacg tacgcaggcc gctggtccgg cggtggcgcc
     7921 tcatacagtc gtgccccgcc cgctcccgtg actatgtagg taataccggt cccgtccggt
     7981 accacctgcc ccccgcgtag cgggtgggtt cgttcgtaaa ggtggtcatg gccgcagaag
     8041 acgaggtcca cgcccgcggc ttccatcgcg gggacgatgg cgcgctggat gcgctcatcc
     8101 gacgggtgcg cgccgcaggt gtacggcggg tggtggaaca caacgaactt ccacgtcgcc
     8161 ggagcggcgg ccaaagtctg tttcagccag ggagcgacct tttcggccag ggttgcttcg
     8221 ggcagcgtgc tatccaggac tacgatgcgc gcggaaccat agtcgaacca gtagttcgtt
     8281 tctggctgca gatctgccgg gccgttgtgc ggcagttcga agatgcccag gtatgggtcg
     8341 ccttggtcgg tcagcacgtc gtgattgccc agcgtaggcc agaacgttat ttgcgatagc
     8401 aaatcgcgat acggtgcgaa gaagcgctct tcgtaattgt tgcgctcacc gcgtttgtaa
     8461 accacgtctc ctgtatgcag caggaaatcg ggcgaaaact ccagtgaccg catgcgctgg
     8521 gcgagcaagt actgctcccg cgtgccgagg ccgctgtcac caaaggcaat gaaggcgaac
     8581 ggttcccccg cggctcgatt ggttcgcaac gtggcttccg ccagcgttcg ccgtccgagt
     8641 tggatgcggt aggggtgcgc cgtaccggcg gtcaagccgg tgatgcgcac tcgttggcga
     8701 gtatcgtttg cctcggccgg gtaatcgcgc gaatcaggcg gggccccgag cgtcaccagg
     8761 caatcacccg gcttgacggg ccggctggtg taccagacca gcgtgacctc gtccgggccg
     8821 gcagcctgaa ccagcggccc ctcaacgatg cggaccggca gaatccaggg cgaagcgaag
     8881 aaaaccagga aacccgcgac gatgaacagc atcgcgccca acaacacggc gcgcgggctt
     8941 gcttggccgc ggcgcatgtt catggctgct ccgcaggtgc gtgggcggcg ggctcggtag
     9001 tcggtgtagc ttgtgggatt aatggggtgc gctcctgagg cggtttctca ccattctctt
     9061 tataccacaa agcgaacagc cgcagggcat tgcggcgcgt cctctcatca agaaagccgg
     9121 tcaccggatg gtcgcccggc gcggacacga atcccagctc atcgggcggc aagccataaa
     9181 tgcgcgccaa ggccagcaac gcggcatcgc ccaccgtgac cctcagcagg ctgcctgccc
     9241 ccagactgcg taccgcgtgc tgttcagtgt ccgtggtgca ctcgagcaac agcggtacag
     9301 cccgctcgtc gccgattttg cccaacgcat ccagcgtctc cgcgacgcgg aaaagcgtgc
     9361 ggtacggcgg gcggccgaaa cagcgaatcg cctcgacgat aaccggcacg ctggagcgcg
     9421 cctttgaatt gcctagaaga cagatggcgc gattcgaaaa atccgcccag gccgcagcct
     9481 cctccgccgg taatgccccg gtcgtgccgc acaccgcctg gcttgccccg tcgacgaagc
     9541 gctctagacc gcgcatggtg gccggatcat ctcccaaccg ggccgctatc ttcagcgccg
     9601 cttcgacaac gagagcgtcc cagtcgaaca gccgcgacgt gaccagcacg ccggccgcgc
     9661 tacccggctc gagcgggatt tgatagagcg ccgccagtcg ccgccgcggg tcgtcgtcga
     9721 acagccgctc gagcaggtcg cgatttccgg ctgcgacctg gtcgaatagt tgtgcgtctt
     9781 ccggcggcag gtttgcctgg tacagacgta ttcgcagcgc cgcgtgaacg cgttgcaggg
     9841 cttgcagaac gcggcgctgc tcggccggag agcgttgctc caggttcttt agaaccgccg
     9901 cgaccggctc aacgatctgc cgcacgaact cgtcggcggc cgccgcggct tcgtcgggag
     9961 tgccgcgcgc gatctggtcg gctagatgcg atagggcggg ctgagtcgac ggcggcacgc
    10021 ttgccggccc cgccgcggac gctacgactg cgatcgcgct caataagacc gcggccgtag
    10081 cacgaccgaa ccgtttgccc ctgggtgtcg tgaggaggat catgttcatc tcccgttgcc
    10141 tcagtgcgcc tgacccaact tacaaccgta actcactgcc cgctccgcgc aaagcagagg
    10201 cggggcgtgt cacccagaaa tgccgccagg aatatcccgc cggcgcagta ccctcggccg
    10261 cgaggcacgc ggggctgggt tgcccttgct ggcgctgctg gctcggatca ggctttgcgg
    10321 gctcgggtcg ggtgcccggc gccccgattt acgagcaatg gtgactgcgt taagcttttt
    10381 ggtgaaaggg agctgacaat gagaaagaac aatcgcgttg tgccttggtt caccggatct
    10441 ggttcgattc tcacgctcgc tggggccgtc atgctcatgc tcgcatgggg ctgctccaaa
    10501 tcggcggaca cgccgccaga gaaaccgcct gccgccccgg ccgccagtcc agaagccggc
    10561 gcgccgcaga ccgctgttga gacgcccgca ccacaaccag ccgctgcccc cgcaacgcca
    10621 ccggccgccg ggaacgtttc gccaccggcc gcggcaccca ctccgccgcc ggccgcaaac
    10681 gttccgccac cggccgcgcc cacggccgga aatgcgccgg gcgcttctgc ggtgcggccc
    10741 gattccgagg aggccaggcg gctgatcgaa gagaagctgg cgcaattgca gtctgccgga
    10801 gcgaagccgg cccctgggca ggctgttacg ccgccgccgt cagccggcgc gccatccgtt
    10861 ccggcgagtg gcgctcaggc cgcgccacag ccccttccgc cgccccctgc accggccgct
    10921 ggtgaacaaa agccgggcag gactcccatt ctggacggcg agtcgctcga catggcgccg
    10981 ccgccgccgg atcagccgca gccccgagcg gtgtacgacc gcgagctgat cgtggcggat
    11041 ccgatctggc gcggcgaatc ggccaagttc ctgttcaacg tgcgaaatga gggcgagggc
    11101 gtgttgaaca tgaagatgaa agcctgctgc ggcttgagca ttaacggggt gcggaacggt
    11161 tggccgggct ggtcgctgag gcccggcgag tccgaaacat tcgagatgac gtttgggccg
    11221 agtgcccgtg gcgatttcac gcgctattcg ccgctgcata cgaacgatcc ccaaaacccg
    11281 aagatcaact tcaccttgaa ggggcgggta ctggtcgcgt ttaacgtgga gccgcacgta
    11341 gtgaatctgg gtgtggttca gccggattcg ggcccgatta ccaaaacgct gaccatcacg
    11401 cgcggcgacg gcggaccgct gcagccgaag ctgaacccgc ctaaagaccc gcgcgtcaaa
    11461 gcggagttga aagaggtcga gcctggggag cgttacacgt tggaggtaac ggtgaccccg
    11521 ccgctgccgc acgattaccg tagttcgctg gtgctggata ccggcgtaag ccaggccccg
    11581 cgcgagacga tcctgatcgt tacccagcat gaggagcatt gagctgccgc ccgcgctaac
    11641 ggcttgcaac gcgcgtcagg gcaggacgcg gtaggggcgg cgcgcttcgt actgcctgat
    11701 ctggaaatcg aggttgcccg gaacgcggct gtcgcgtggg aagtcggccc gaatctgccg
    11761 cgcgatgcgc accgcttcct caaagcgacc ggtttcggcg tacgccgccg ccaaggctgc
    11821 ggcgctccgc gggtccgggt cgccgctacg gaccgcgaac tcggccaatt cgagagctcg
    11881 cggtccatcg cgcaagtttt catcgtgggt ggtggattgg taccaggcca gcgcggcgca
    11941 gagtcgcggg tcggcgggct tggcggcgtg tgcttccttc aataccttga gcgcttctct
    12001 ctctcgcccg cccgcggcca ggaggtcggc ccaattgcaa aagacctcgg ggtcagaggc
    12061 atcctcgttc caggcgcaat acgcctcggc ccgggcgatt ccctcctgct ggcggcgcag
    12121 gttgtccagc aaggcggata acatctgctc cgcctgcccg tggttgggtt cacgctcgag
    12181 cacttgttgg cacagctcga tgccatgcgc atagcggccg cgcgacgcca gcgtgagcac
    12241 cagatcaagc gcggcctggc ttgactccgg atacagttgc agggcctgcg tgagcagtaa
    12301 ctggctctca tccagccggt tgaccaggcc atagcagttc cccagcaggt ggagaatggc
    12361 aaacgactca ggactctgtt cggcggctgg ctcgagcagc ttgatggcct cggccggggc
    12421 gtgaggcgcc agctcctggg cggtgcggta atcgcgctcg acggcgatca tgtcgcgcgg
    12481 gttctttagt gccggcgcgg tgctggacgg gctgggcgtg tcgcctacgt agcccaggct
    12541 gcgcagggcg cgctcctgga cggagtccgg tttaacggac actcccgcgc ggcgctgtac
    12601 ggcgtgttcg aaggcctgca actccgactc gagctgcgtg acgacctcgg ggcgggcggc
    12661 ggccacgttc gacatctggc gcgggtcagc ttgccggtcg tacagttcgt tgcgcggggc
    12721 gcggatgtaa acccactggg gggtagtgat actgtaaagc gcgctccaac cgaagctgta
    12781 gaacgggtac tccgtctcgc cgtaaatagc gcgcgacgcc ggcggccggc cggcgagggc
    12841 cgacaaacca ctgacgccgc tgacggtctg cggcacgggc cagccgacga gttcaagcac
    12901 ggtgggcatg atgtcggaaa tggcgaccaa gtccgcgcac acgacgttct gcgggagccg
    12961 cccgggcaac gacataatca gcggcacgtg catcaattcg acgttgagca gcagcgtgtg
    13021 caagtcgtat ccgtggtcgc ccagcccctc gccgtggtcg gccacggcga ttacgagggt
    13081 tcgttcgcgc aggttgttga tttgcagcca gtcgatcagg cggccgatgt tggcgtccat
    13141 gaaggcgact tcgccggcat agggcccgcc ggcacgggcg gcatactcct caggcggggt
    13201 gtaggggtca tgcggatcga agtagtgcac ccagcagaag aagcgctggt cacgcacctc
    13261 ttccaaccag gccaaggcct ggttgctcat gctgtgggcc gaatgcgagc gcaaaatctc
    13321 gccggacggg ctgatgggta tgtcgtcgcg atagttctcg aacccgcggt tcagaccata
    13381 gcgcgcctgc aaaatggcgc acgccacgaa agcgccggtg cggtagccgt gctggttgaa
    13441 gatttcggcc agcgtgggca gttcgggccc gagtgcgaaa cgggtgttgt cgcgtacgcc
    13501 gtgctcgggg ggcagcgtgc cggtcatgag cgaagcatgg gcggtgagcg tgatgggggc
    13561 gtgcgtgaag gcgttgctga agcgcacacc ggtgcgggcc aggttgtcca ggaccggcgt
    13621 caagttccct ggatggccgt agcagccgag gtgatcggcg cgggtcgtat ccatggtgat
    13681 aagcaggaca ttgcaatcgg cggcacggcc ggcgagcttg tcggtggcgg ccggcttttt
    13741 ggagcagcct acgcaaaggg ccagcggaac ggcggttacg agcaggaa
//
LOCUS       DAOSYS010000077        13706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3781_length_13706_cov_4.849095, whole genome shotgun sequence.
ACCESSION   DAOSYS010000077 DAOSYS010000000
VERSION     DAOSYS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_13706_cov_4.849095"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PLU99_06485"
     CDS             <1..345
                     /locus_tag="PLU99_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO16741.1"
                     /translation="IVEMTHVEKLAERILFLKGDVKMVPSGPVDTATEATEILTRAAA
                     SEQKAVRDYNKAALECSANADAASKQIFESLVVDEEGHFDAFDKQLENIKRFGPSYLA
                     LQSFDGGESKSD"
     gene            320..1213
                     /locus_tag="PLU99_06490"
     CDS             320..1213
                     /locus_tag="PLU99_06490"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOO16742.1"
                     /translation="MAANRNPIDATQAEQTGRDAPAEQSARPGAGADLARRRLRQRRL
                     RRILAVALALFVLVAVADLAFRPVGRIVPPPPGAEPGRWVFLEGAANTRDLGGYTTVD
                     GRTVRRGRIYRSGNLGHLSSAGVSVFRDLGVTTVIDLRNRLTPLPWSNGDVLGVQLAA
                     RVHGCPMSFEKPADRSQFYVLGVQENQAAVRRAFELLAQSDGPVLYHCAIGVDRTGVL
                     SALLLRLLGVSRADTLADFRLSEQIGTPGNLNAMNRLLDEVERQGGIEQYLLNIGVSA
                     ECQAQLRQKLLEPTPLAPPPH"
     gene            complement(1328..1681)
                     /locus_tag="PLU99_06495"
     CDS             complement(1328..1681)
                     /locus_tag="PLU99_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16743.1"
                     /translation="MPEMFKRLVAAAAALSWVAMIAGCESRGARQPAFEDRAVSESEL
                     AKIEAETAELRRQAAALAAEHERMEAEHAAAKAEAEELQRLIEQVRKAQEAEAAQRAE
                     EAQQRRSGSNNAHPQ"
     gene            complement(1832..4324)
                     /locus_tag="PLU99_06500"
     CDS             complement(1832..4324)
                     /locus_tag="PLU99_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOO16744.1"
                     /translation="MSTPLERPALIVVTSCMLLALPSAVAVAILKRRRGDNRRGRFLG
                     GLVLILAVGAGVLRHQAAVAKPPNHVTHLLADEPLLTRLVGDLESMPTITPGERHNPF
                     IRFDPPARTQFIIAARELRTTSPPTPVCGLIRVTVRDEGLVLRLGERVQLTGRLYRPT
                     PPRNPGEFDWARWNYLQGIEAGMVVEGAEHVRRMPVEPSWLARVVAGARLYAHLLLLG
                     SYGDEPPDAAGRLLDTLVLGQRTAADRELNEAFLRAGGLHFLAVSGFNIAVLAGASCW
                     VMRGVLRRSARTTAAVTLCSVVIYGTVAEPNAPIVRATVLGVLVALSGLARRQATIWN
                     GLAASALCVLLVNPLELFRSGFQLSFVQVMALILLVGRAMAPNPTRFFERPREEEAGS
                     WRAFIRMKLWREAVGLVVLSVIAWAVATPLVLFHYGRLAPWGFIGSPLLTIPVVLITW
                     LGVLTIIMQALCQPAAGLVSMPLQWLTSGVLWAVHCFANLARPGVVELAKPPFVLVFG
                     TYGLLGAAVAISGRLRLEQWVADFRIRRRARRRTLLVWSVTLGVLLLAWGGWLLWPRG
                     GGKDFELHVLSVGGGNAMMMITPAREAVLFDIGTDRNSNAAELVGQAAWALGVRRVAG
                     VAVSHANFDHCSGLATALDLLSIDMLVLSKYTGSPKDTTPAALQALTPSGRSKLVRVH
                     AGDSLTLAGMPVEVLWPPADADAAAWTANDLSLVLRVHAGPRTVLFTGDIEGRALREL
                     LAADAAGTIHLASDVLIAPHHGSLVPESAAFYAAVNPQVVVASSAEERPKLAALVDSA
                     LGAEAQLLSTARSGAVTVSVQPDGELRLETAW"
     gene            4789..5814
                     /gene="gnd"
                     /locus_tag="PLU99_06505"
     CDS             4789..5814
                     /gene="gnd"
                     /locus_tag="PLU99_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOO16745.1"
                     /translation="MQLGMIGIGRMGGNMARRLMRAKHEVLVYDPVTQTRESFANETG
                     AFAADSIEQLVSALEPPRVVWLMVPAAAVEEVVGELARHLERDDTIVDGGNSYYVDDI
                     RRARELANKGIHYVDCGTSGGVWGLERGYCLMIGGEEAVVRRLDPILAALAPGIGGAP
                     RLPGREHVGGTAELGYLHCGPSGAGHFVKMIHNGIEYGIMAAYAEGLNILRHANAGKT
                     EQAADAESAPLRNREEYSFDFNLRDITELWRRGSVISSWLLDLTASALLQDPTLAEFE
                     GRVSDSGEGRWTIKAAIDEAVPAPVLTAALYERFTSRGRAEFANKLLSAMRFGFGGHM
                     EEQPAGT"
     gene            complement(5862..7106)
                     /locus_tag="PLU99_06510"
     CDS             complement(5862..7106)
                     /locus_tag="PLU99_06510"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO16746.1"
                     /translation="MSPTFAYHELPNGLRVVCQTLPQVHSAAIGFYVRTGARHEQPDL
                     HGVSHFLEHMCFKGTATRSSRDINVCFDELGSIYNAFTGKEHTVYYGWVPAAHLTRQL
                     ELLADMMHPALPPDDFETERKVILEEIAMSDDSFDRHLWDFLHRRVFGQHPLGHEILG
                     EKETIQSLPREAMAGYHQARYSPQNVVVLAAGAIDPQELFAGVGRFCGDWPRHTDDEL
                     PNEPMPPFQPGTAKLHLPRFKQQALVRLYPSVPHGHPAGESIEAFVSLFGGSNSRCYW
                     NIVQQGICTGAGAAWVSYDDCGLLALYAEGEPERCEEMLEALDEQTRAVAEGGFTPEE
                     VQRVKNRRRTQLALEAENPRTRLMQMLDDFEATGYVRTVEARLAAVEAVSAKTIADYL
                     KRYPITGDGLLLSCGPRDWPPT"
     gene            complement(7380..>8831)
                     /gene="der"
                     /locus_tag="PLU99_06515"
     CDS             complement(7380..>8831)
                     /gene="der"
                     /locus_tag="PLU99_06515"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOO16747.1"
                     /translation="GHARARPAGLGMAPANMGPVGLRHGPAPRRRPEIPIRAARAYHR
                     GSMALPVVVIVGRPNVGKSSLLNNLAREHISIVDPRAGITRDRVSTIVEYNDRYFELV
                     DTGGIGIIDDQQLEEHVEQQIHYAIARADVIIFLVDVADGVTPLDVSVAELLRRVNKP
                     VILVANKVDGPAREPEAAAFFRLGFGEPLCASALHGYGREALLERLGQLLGPAVGERP
                     EEPVMKLAIVGKRNAGKSTLVNALAGEDRMIVSEIPGTTRDAVDVQFERDGRQFVAID
                     TAGVRKRASMRDVDFYSYTRALRSIRRADVVMLLIDAIVPVAEVDLKLARAVLDEYKP
                     VLIAINKWDLAKDQATTGQFADYLTETIPWLRYAPIIFMTAKTGHNVQAAVDVAASLH
                     KQAQTRVGTGKLNQALQKILEHRGPSAKHGTQPVKVYYATQVAVAPPTVVFFCNNAGA
                     VTENYRRYMENRLREHLPFSEIPVRLLFRTRSA"
     assembly_gap    8832..8931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9071..9826)
                     /locus_tag="PLU99_06520"
     CDS             complement(9071..9826)
                     /locus_tag="PLU99_06520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO16748.1"
                     /translation="MPTEHSTGPSFSPDAAPPNTRLTDQAAAQAARTRHEHFKHTARA
                     HFDRWALSYDRSWLGELVFYPSMRACHEEILRWQAERGRAPYRLLDVGCGTGNLLLSL
                     VDDPHAEQLVGLDYSEEMVRCVQMKIDPEIHLDRLRVVHGDAEHLPFEDASFDVLTCA
                     NSFHHYPHQEAVIRQFYRVLRPGGMLVLIDGFRDNVVGWIVFDVAVAFVEKAVHHAAW
                     SAVRNMLAEAGFSTITQRKLNVLAPLLVSVAKK"
     gene            complement(9832..10821)
                     /locus_tag="PLU99_06525"
     CDS             complement(9832..10821)
                     /locus_tag="PLU99_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005912141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOO16749.1"
                     /translation="MPEWYYAANGQSFGPFTREELLAKLPSLQGPDTLVFGPSVSYWT
                     PARRVAGLVGAAAAPPPPGAGRCDEVDYKITGDDMQFVEVELDPGEMVIADAGAMMYM
                     TAGIRMETRFGDPSRPEQGLLGKVFSAGKRVLTGESLFLTTFVNGGSGKHQVAFAAPY
                     PGKILAMDLSQLGGELLCQKDSFLCAARGVSIGVAFTKRIGAGLFGGEGFVLQRLSGS
                     GLVFVHAGGTLHRRSLAPGETLRVDTGCLVAFQPSVEYDIGFVGGLRNALFGGEGLFF
                     VTLTGPGEVWLQSLPFARLADRIHAAGGVTGRRPDEGGSILGGLGRVLDGDNR"
     gene            complement(10909..11457)
                     /locus_tag="PLU99_06530"
     CDS             complement(10909..11457)
                     /locus_tag="PLU99_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16750.1"
                     /translation="MRKLLAAAIIIGVSLGIAGCAQNAYVGHQGGWAGVWFGDVGVKG
                     NGNNVNVERGSNLRKLSILGDCNTVNVEDGVTLVHVEFFGKNNTVTIPAGLLIRLTDW
                     GQGNTLVRRPEVWDRMIETPYGYTPAVIPLGPATPMPTETTPPGGTPLPTGTPVAPPP
                     AEPRPDVQLVPDEFEQMEEPTK"
     gene            complement(11624..13144)
                     /locus_tag="PLU99_06535"
     CDS             complement(11624..13144)
                     /locus_tag="PLU99_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HOO16751.1"
                     /translation="MPAEPNGTEFEFVAGQKPRTPSDVLLVPLVTKPPPPLPLVKAVD
                     DACGDAVSALLAVQAVGAEAGHLAHTTSAGPVPRILVVDLGPAEKLTGLVVRQAAASA
                     ARWLIAEKLTSGAVWLDGLASTGLNECFREWTLGMALAGFRFTEFLKNNNRTPKKVRL
                     QLRAADPGLVRRAMGEIKTATTIAAAVNYARRLAHLPGNVINPQTLAEEAKRLARHCG
                     LKYKLVDAAEARRMGMGGLLAVGGGSAHAPCLIQLEYRPRPDSRRNTVLVGKAITFDT
                     GGYSIKPAAGLEGLKFDKCGGAAVLGALQAASQLGLPCNVAGVIGAAENAISERAYRP
                     GDILTMASGKTVEVISTDAEGRLVLADALWYAQQHLNPTEIIDIATLTGGVTVALGKV
                     AAGIMSTDDELAGVLGECGRAVHERLWRLPLWDEYRELIKGTDADIRNSSGKREAQAI
                     VGGMFLKEFIARGMAWAHVDIAAVATLEESNSPTGKGATGFGVQLLVEYLRRAAQP"
     gene            complement(13286..>13706)
                     /locus_tag="PLU99_06540"
     CDS             complement(13286..>13706)
                     /locus_tag="PLU99_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase GatDE subunit D"
                     /protein_id="HOO16752.1"
                     /translation="QPDLVDAVVEKGYRGIIIAGTGLGHVNKPLYPALRRAIEAGVHV
                     YMTVQTLWGYVQMYVYSTGRDLMELGVVPAANMLPEVAYMKLCWALGQSHDREEVKRI
                     MLTPIIDEITLREPYNGYLIYQGEIPEVQEFLRSIRR"
BASE COUNT         2376 a         4468 c         4400 g         2362 t
ORIGIN      
        1 atcgtcgaga tgacgcacgt cgaaaaactc gccgagcgca tcctgtttct caagggcgac
       61 gtgaagatgg tccccagcgg tccggttgac accgccaccg aggccacgga aatcctcact
      121 cgcgccgcgg cttccgagca gaaggcggtc agagactaca acaaggccgc cctggagtgc
      181 agcgccaacg ccgacgccgc gtcaaagcag attttcgagt ctctggtggt ggacgaagag
      241 ggccacttcg acgccttcga caagcagctt gaaaacatca agcgcttcgg acccagctac
      301 ctggccctgc agtcattcga tggcggcgaa tcgaaatccg attgacgcca cgcaggcgga
      361 gcaaaccggc cgtgacgcgc cggctgaaca gtcggcgcgc cccggcgcag gggccgacct
      421 agcgcggcgg aggcttcgtc aacgccgcct ccgccgaatt cttgcggtcg cactggcact
      481 tttcgtgctc gtcgcggtcg cggacttggc gtttcgcccc gtcggacgca tcgtcccccc
      541 gccacccggc gccgaacccg gccgctgggt cttcctcgag ggtgccgcca atacccgcga
      601 cctcggcggc tacactacag ttgacggccg caccgtaagg cgcggccgga tctaccgctc
      661 cggcaacctg ggccacctct cctcggccgg cgtgagcgtc tttcgcgatc tcggcgtcac
      721 caccgtgatc gacctgcgca accggctcac acccttgcca tggtccaacg gcgacgtcct
      781 cggcgtacaa ctcgccgccc gcgtccatgg ctgccccatg agcttcgaga agcccgccga
      841 ccgctcgcag ttctatgtcc tcggcgtgca agagaaccag gccgcagtgc ggcgtgcctt
      901 cgagttactc gcgcaatccg acggccccgt tctatatcac tgcgcgatcg gcgtggatcg
      961 caccggtgtt ttgtcggcac tgttgctgcg tctgctgggc gtttcccgag ccgacacgct
     1021 ggcggatttt cgcctttccg agcaaatcgg tactcccggc aacctgaacg cgatgaaccg
     1081 gctcctggat gaagtcgagc gacagggtgg aatcgagcag tacctgctga acatcggcgt
     1141 ttcggccgag tgccaggcgc agttgcggca gaagctcctg gaaccgacgc cattggcccc
     1201 gccgccgcac tgatcgggaa cagcgtgcca catcgcaggg ccggggtaat agtagttagt
     1261 tgttagttat tagtattgag aaggggggta ccgctggtca atggtgggtt tgcttcccga
     1321 agctcgacta ttgaggatgc gcattgttcg accccgagcg cctctgttgc gcttcctccg
     1381 cgcgctgcgc cgcttccgct tcttgcgcct tccgcacctg ttcgatcagc cgctgcagct
     1441 cctccgcttc cgctttcgcg gccgcgtgct cggcttccat gcgctcgtgc tcggccgcca
     1501 gggccgctgc ctgtcgccga agctccgctg tttccgcctc aatcttcgcc agctctgatt
     1561 cgctgaccgc acgatcttca aacgccggct ggcgcgcccc gcggctctcg cagccggcga
     1621 tcatcgccac ccaactcaga gccgctgcgg ccgccaccag ccgcttgaac atttcaggca
     1681 cgggcatact cctgcggagc ccttgcgcgc atggctcacc gccgctcgcg ggtctcaaat
     1741 cgccaatata gccgatggtt agccaaaaag tgcgggcccg cgatgacttc gagaacagtc
     1801 gcgagcatat tccacaaacc ccggcctccc tttaccaggc tgtctccagc cgcagttcgc
     1861 cgtccggctg aaccgacacg gtcaccgcgc ccgaacgcgc cgttgacagc agttgagcct
     1921 ccgcgcccaa cgcactatcg accagcgccg ccagtttcgg ccgctcctca gccgaggaag
     1981 cgaccacgac ctgcgggttg acggcggcgt aaaaggccgc cgactcgggc accaggctgc
     2041 cgtggtgcgg cgcgatgagc acgtccgagg ccaagtgaat cgtcccggcc gcatccgccg
     2101 ccagcagttc acgcaaggcg cggccctcga tatctcctgt aaacaggacg gtgcgcggcc
     2161 cggcgtgcac gcgcagcacg agcgagagat cgttggccgt ccaagcggcg gcgtccgcgt
     2221 cggccggcgg ccaaagcacc tcgaccggca tgccggcgag cgtgagactg tcgccggcat
     2281 ggacacgcac cagtttagat cgcccggacg gcgtgagggc ctgcagcgca gccggcgttg
     2341 tgtctttcgg cgatcctgtg tacttgctca aaaccaacat atcgatcgac agtaagtcga
     2401 gcgcggtggc caaaccgctg caatggtcga aattcgcgtg cgaaaccgcg acgcccgcca
     2461 cgcgccgcac gcccagcgcc caggccgcct gtccgacaag ctccgccgcg ttcgaattgc
     2521 ggtcggtgcc gatgtcaaac aaaaccgcct cgcgggcagg cgtgatcatc atcattgcat
     2581 tgccgccgcc tactgacagc acgtgtaatt caaaatcttt tcccccgcct cgcggccaga
     2641 gaagccagcc gccccacgcg agcagcaaca cgcccaacgt cacagaccag acgagaagag
     2701 tgcgccgccg cgccctgcgc ctgatgcgga agtcagccac ccactgctcc aggcgcagtc
     2761 ggcccgatat ggccacagcc gcccccagca gtccatacgt gccaaacacc aacacgaacg
     2821 gtggcttcgc cagttcgact acccccggcc tcgccaggtt cgcaaagcaa tgcaccgccc
     2881 acagcacccc actggtcagc cactgcagtg gcatgctcac cagtccggca gccggctggc
     2941 acaatgcctg catgatgatc gtcagcacgc ctagccaggt gatgagcacg accgggatgg
     3001 tcagcagtgg cgagccgata aagccccacg gtgccagccg tccgtaatga aacaggacca
     3061 gcggcgtggc gaccgcccag gcgattacgg acaacaccac aagccccacc gcctcacgcc
     3121 acagcttcat gcgtatgaag gcacgccacg acccggcttc ttcttcgcgt gggcgttcga
     3181 aaaaccgtgt ggggttcgga gccatcgcac gtccgaccaa caggattaga gccatgacct
     3241 ggacaaacga aagctgaaac cccgagcgga aaagctccag tgggttcacc agcagcacac
     3301 acagcgcgct ggccgccagc ccgttccaaa tcgttgcttg ccgcctcgcc agcccggaca
     3361 aggccaccag cacgcccaat actgtcgcgc gcacaatcgg cgcgttcggc tcggctacgg
     3421 tcccatagat cacgactgaa cacagcgtta ctgccgccgt agtacgtgca ctgcggcgca
     3481 ggacgccgcg catcacccag catgacgccc cggcgaggac cgcgatattg aacccgctca
     3541 ccgccagaaa gtgcagtccg ccggcgcgca gaaaggcctc attcagctca cggtcggccg
     3601 cagtgcgctg ccccaggact agtgtatcca gcagccggcc cgccgcatca gggggctcgt
     3661 cgccataaga gcctaacagc aggagatgcg cgtacagccg ggcgccggcg accacccgcg
     3721 ccagccaact cggctctacg ggcatgcgcc tgacatgttc cgccccctcc acgaccatcc
     3781 ccgcctcgat gccctgcaaa tagttccagc gggcccagtc gaattcgcca gggttgcgcg
     3841 ggggcgtcgg ccggtatagc cggcccgtta actgcacccg ttcgcccagg cgcaatacca
     3901 agccttcgtc acgcacagtg acgcgaatca agccacacac cggggtgggc ggcgaagtcg
     3961 ttcgcagttc gcgggcggcg attatgaatt gcgtacgggc cggcggatcg aatcgtatga
     4021 agggattgtg gcgttcgccc ggcgtaattg tgggcatact ctccagatcc ccgaccagtc
     4081 gcgtcaatag cggctcatct gcaagcaggt gggtcacatg attgggcggc ttcgctaccg
     4141 ccgcctgatg ccgcagaact ccggccccga ctgccagaat cagtaccagg ccaccgagaa
     4201 agcggccgcg tcgattgtcg cctcggcggc gcttcaggat tgccaccgct acggcactgg
     4261 gcagcgccag gagcatacat gacgtcacaa cgatgagcgc cggacgttcc agaggcgtag
     4321 acatttccgc aagccagaca ccgccaataa atgcaatggc cggccacagc agcggagctg
     4381 gatcgcggtg catacggtca cctgcggccc gatcgcgcat acgcagcggt aatgcctgcg
     4441 tctgactggt ggacatggtt gcattatgaa tgtgtgtggt gacaaagacc actgcgggcg
     4501 ttcagcgatg ccggccaatc cgagcgccat tgtcgggtat gtggtccggt attgaggccc
     4561 gtagcgacca ggtgacctgc tcctcacggc ggcggcacag gccgagatga acgggcgttc
     4621 aaacgcaaaa ccggccaaag tacgttcgtt ttgtcgagcg gcgcgaggtt cgcaaataat
     4681 gagtggtagt gtaatgaggg ttcgatggcg agcgccgccg gtagcccgct cgcagcggca
     4741 tttgttgcgc cgcttccctg acggcggacc ttacgcaagg agccatgtat gcaactcgga
     4801 atgattggca tcggccgaat gggcggcaat atggcgcgcc ggctgatgag agcgaagcat
     4861 gaagtgctcg tctacgaccc cgtaacgcaa acgcgcgaga gcttcgccaa cgaaaccggc
     4921 gctttcgccg ctgactcgat cgagcagctc gtctcagccc tagaaccgcc gcgcgttgtc
     4981 tggctgatgg taccggccgc ggccgtggag gaggtcgtgg gcgaactcgc gcggcacctg
     5041 gagcgcgacg acacgatcgt ggacggcggg aactcatact acgtcgatga catccgccgc
     5101 gcccgcgaac tggcgaacaa gggcattcac tacgtggact gcggcaccag cggcggagtg
     5161 tggggcctgg agcgcggcta ttgcctgatg atcggcggcg aggaagccgt ggtgcggcgg
     5221 ctcgacccga tcttggccgc gctggcgccg ggcatcggcg gcgcgccgcg cctgcccggg
     5281 cgagagcacg tcggtggaac ggccgagctt ggctacctgc actgcggccc cagcggcgcg
     5341 ggccatttcg tgaaaatgat tcataacggc atcgagtacg ggatcatggc cgcgtatgcc
     5401 gaggggctga acatcctgcg gcatgcgaac gccgggaaga ccgagcaggc agccgacgcg
     5461 gagagcgccc cgcttcgcaa ccgcgaggag tacagttttg acttcaacct gcgcgacatc
     5521 accgagctgt ggcggcgcgg gagcgtaatc tcgtcgtggc tactggattt gaccgccagc
     5581 gcgctgttgc aggacccgac actggcggaa ttcgaaggac gcgtgtctga ttccggcgag
     5641 ggacgctgga cgatcaaagc cgccatcgac gaggccgtgc cggcgcccgt gctgacggcg
     5701 gcgctgtacg agcgcttcac ctcgcgcggc cgggcggaat tcgccaataa gctactttcg
     5761 gcgatgcgct ttggcttcgg cgggcatatg gaggagcaac cggccggcac ttagcgagca
     5821 cggcagcgac tgccagggag catccgaggc gtcggcggcg attacgtcgg cggccagtcg
     5881 cgtgggccac aggacagcag caggccgtcg ccggtgatcg ggtagcgctt caagtaatcc
     5941 gcgatagtct tggcgctgac ggcctcgacc gccgccaggc gcgcctcgac ggtccgcacg
     6001 tacccggtgg cttcgaagtc atcaagcatc tgcatcaggc gcgtgcgcgg gttctcggcc
     6061 tccagcgcca gttgcgtgcg gcgccggttc ttgacgcgct gcacttcctc gggcgtgaag
     6121 ccgccctcgg ccacggcgcg ggtttgttcg tcgagggctt ccagcatttc ctcgcagcgc
     6181 tccggctcgc cttccgcgta cagcgccagc agcccgcaat cgtcgtaaga cacccaggcc
     6241 gcgcccgccc cggtgcagat gccctgttgc acgatgttcc agtagcagcg cgaattgctg
     6301 ccgccgaaga gcgaaacgaa cgcctcgatc gattcgccgg ccgggtggcc atgcggcacc
     6361 gagggataga gccgcaccag cgcctgttgc ttgaagcgcg gcagatgcaa cttggccgtg
     6421 ccgggctgga aaggcggcat cggctcgttg gggagttcgt catccgtgtg ccgcggccag
     6481 tcgccgcaga agcgcccgac gccggcgaag agttcctgcg ggtcgatcgc gccggccgcc
     6541 aggacaacga cgttctgagg cgagtagcgg gcctggtggt atccggccat ggcctcgcgc
     6601 ggaaggcttt gaatggtttc cttctcgcct aggatttcgt gccccagcgg gtgctgcccg
     6661 aagacccggc ggtgcaggaa atcccatagg tgacggtcga agctgtcatc gctcatcgcg
     6721 atttcttcga ggatgacttt gcgttcggtc tcgaaatcgt ccgggggcag cgcggggtgc
     6781 atcatgtcgg ccagcaactc gagctgccgc gtcaggtggg cggccggcac ccagccgtaa
     6841 tagactgtgt gctccttgcc ggtaaaggcg ttgtagatgc tgccgagttc gtcgaagcag
     6901 acgttgatgt cgcgggagct gcgggtggcc gtgcccttga agcacatgtg ctccaggaaa
     6961 tggctgacgc cgtgcaggtc tggctgttca tgccgcgcgc cggtgcgaac gtagaagccg
     7021 atggcggcgg aatgcacttg cggcagagtc tggcaaacga cgcgcaggcc gttgggcaac
     7081 tcgtggtagg cgaaggtcgg tgacattcgg gggattataa ggcgtagagc ggggacgtgc
     7141 agagtgggga ccgagggatc tagggatcaa gggattgagg gatcaagaga gcagagccgg
     7201 ggcgtgtcgc cccggtgaat atcagttgcc ttcgtggcgt tcggcgactc gcggtgtcga
     7261 gctggcaccc gctggggtta ctggcgatgg catgttactg acgatggcat gcccgcgccg
     7321 ctacgcggct tgggcatggc acccgggctt ccgctgcgtt ttgacgatgc ccgtccgcct
     7381 taagccgatc gggtgcgaaa cagcaggcgg acgggaattt ccgagaacgg caggtgctca
     7441 cgcagccggt tttccatgta gcggcggtag ttctccgtca ccgcgccggc attgttgcag
     7501 aagaacacta cggtcggcgg cgcaaccgcg acttgcgtcg cgtagtacac cttcaccggc
     7561 tgcgtgccgt gcttggccga tgggccccgg tgttccagaa tcttctgcag cgcctggttg
     7621 agctttccgg tgccgacgcg cgtttgggcc tgcttgtgca agctggcggc cacgtccacc
     7681 gccgcctgga cgttatggcc ggttttggcg gtcataaaaa tgatcggggc gtagcgcagc
     7741 caggggatag tctcggtgag gtaatccgcg aactggccgg tggtggcctg gtctttggcc
     7801 aggtcccatt tgttgatggc gatgagcacc ggcttgtact cgtccaggac cgcccgggcg
     7861 agcttcagat cgacctcggc caccggcaca atggcgtcga tcagcagcat gacgacatcc
     7921 gcccggcgga tcgaacgcaa ggcgcgcgtg tacgaataga aatccacgtc gcgcatgctg
     7981 gcccgcttgc gaacaccggc cgtatcgatg gcgacgaact gccggccgtc gcgctcgaat
     8041 tgcacgtcca cggcgtcgcg ggtcgtaccg gggatttcgc tgacgatcat ccggtcctcg
     8101 ccggccaggg cgttgaccag cgtgctcttg ccggcgttgc gcttgccgac gatcgccagc
     8161 ttcatgaccg gctcttcggg ccgctcgccg acggccgggc cgagcagttg gccgaggcgt
     8221 tcgagcagcg cttcgcggcc gtagccgtgc agagccgagg cgcacagcgg ctcgccgaaa
     8281 ccgaggcgga aaaaggcggc cgcctcgggc tcgcgggcgg gaccgtcgac cttgttggcg
     8341 acgaggatga cgggcttgtt gacccggcgc aggagttccg cgacggagac gtccagcggg
     8401 gtgacgccgt cggcgacgtc gaccaggaag atgatcacat ccgcgcgggc gatggcgtag
     8461 tgaatctgtt gctcaacgtg ctcttcgagc tgctggtcgt cgatgatgcc gatgccgccg
     8521 gtgtcgacga gttcgaagta gcggtcgtta tattcgacga tggtggagac ccggtcgcgc
     8581 gtgatgccgg cgcgcggatc gacgatgctg atgtgctcgc gcgcgaggtt gttgagcagc
     8641 gagctctttc cgacgttcgg ccggccgacg atgactacta caggcagcgc catagatccg
     8701 cgatgatacg cgcgcgcggc gcggatggga atttcggggc gcctgcgcgg cgccggcccg
     8761 tgacgaagtc ccacaggccc catgttcgcg ggtgccatgc ccaagcccgc ggggcgggcg
     8821 cgggcatgcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcccccgcg
     8941 cgacgccgtg gaccgattcg aatgtctttt gatcgtgtct ctcgatctgg catgcccgcg
     9001 cttagagctt gggcatggca cccgcgtttt gggcatggca cccgacctta ggcggcacgc
     9061 cccggctttg ctactttttg gcgacggaga cgagcagggg ggctaggacg ttcagtttgc
     9121 gctgcgtgat ggtgctgaag ccggcttcgg ccagcatgtt gcggacggcc gaccaggcgg
     9181 cgtggtggac cgccttttcg acgaaggcca cggcgacgtc gaagacgatc cagccgacca
     9241 cgttgtcgcg gaagccatcg atcagcacca gcatgccgcc gggccgcagc acgcggtaaa
     9301 actggcggat gaccgcctcc tggtgcggat agtggtggaa cgagttcgcg caggtaagaa
     9361 cgtcgaaaga ggcatcctcg aacggtaggt gttcggcgtc accatgcacg acgcgcaggc
     9421 ggtccaggtg aatctcgggg tcgatcttca tctgcacgca ccgcaccatt tcctcggagt
     9481 agtcgaggcc gacgagctgc tcggcgtgcg gatcatcaac taggctgagc aggagattgc
     9541 cggtaccgca gccgacatcc agcaggcgat agggagcgcg gccgcgctcg gcctgccaac
     9601 ggagaatttc ctcgtggcag gcgcgcatgc tgggatagaa gaccagttcg ccgagccatg
     9661 agcgatcgta cgacaacgcc cagcggtcaa agtgcgcgcg ggccgtgtgc ttgaagtgct
     9721 cgtggcgggt gcgggcggct tgagcggcgg cctggtctgt caagcgcgtg tttggcggcg
     9781 ctgcgtccgg gctgaaggac gggccggtgc tgtgttccgt tggcatttca ctcacctatt
     9841 gtccccgtcc agcactcgcc ccagcccgcc caggatgctg cccccctcgt cgggtctccg
     9901 ccccgtcacg ccgccggccg cgtggatgcg gtcggccaag cgcgcgaagg gcaatgattg
     9961 cagccagacc tcacccggcc cggtgagcgt aacgaagaac aggccttcgc cgccgaatag
    10021 cgcgttgcgc aagccgccca cgaagccgat gtcgtactcg acgctgggct gaaacgcgac
    10081 caggcagccg gtgtcgaccc gcagcgtttc gccgggcgcc aaactgcggc ggtgcagcgt
    10141 gccgcccgcg tgcacgaaga ccaggcccga gccgctcagc cgctggagaa cgaagccctc
    10201 gccgccgaac agaccggcgc cgatgcgctt ggtgaatgcg acgccgatac ttacaccgcg
    10261 cgccgcgcag aggaatgaat ccttctggca cagcaactct ccgccgagct gcgaaaggtc
    10321 catggccaga atcttgcccg ggtacggcgc ggcgaaagcg acctggtgct tgccggaacc
    10381 gccgttgacg aaagtagtca ggaacaggga ttcgccggtg agcacgcgtt tgccggccga
    10441 gaacaccttg ccgagcaggc cctgctcggg ccggctcgga tcgccgaagc gcgtttccat
    10501 gcggatgccg gcggtcatgt acatcatggc gccggcgtcg gcgatgacca tttcgccggg
    10561 atcgagctcg acctcgacga actgcatgtc atcgccggtg attttgtagt cgacctcatc
    10621 gcacctgccg gcgcccggtg ggggcggggc cgcagcggcg ccgaccagcc cggccacgcg
    10681 gcgggccggc gtccagtaac tgacacttgg gccgaagacc agcgtgtccg ggccttgcag
    10741 agagggcagc ttggctaata actcttcgcg tgtgaagggc ccgaaggatt gtccgttcgc
    10801 ggcatagtac cactctggca ttgccattca atgccgtcct tcgtcgcaga cgcatgtagt
    10861 gcgctgcgtc atttaaccgg ccgtcgctat cgccactcga cggttcggct atttcgtcgg
    10921 ctcttccatc tgctcgaatt cgtcgggaac gagctgcacg tcagggcgcg gctcggccgg
    10981 aggcggcgcg acgggcgtac cggtcggaag aggcgtgccg cccggaggcg tcgtttcggt
    11041 tggcatcggc gtggccggcc ccagtggaat aacggccggc gtgtagccgt agggggtttc
    11101 gatcatgcgg tcccagacct ccgggcgtcg cacgagggtg ttgccctggc cccagtcggt
    11161 gaggcgtatg agcaggccgg cggggatggt gacggtgttg ttctttccga agaattcgac
    11221 gtgcaccaag gtgacgccgt cttcgacgtt caccgtgttg cagtcgccca agatcgagag
    11281 cttgcgcaag tttgatccgc gctccacgtt gacgttattg ccgttgccct tgacccccac
    11341 gtcgccgaac cacacgccgg cccatccacc ctgatgcccg acgtaggcgt tctgtgcaca
    11401 gcctgcaatg cctagtgaaa ctccgataat gattgcggca gccagcaact tcctcatagt
    11461 aatgctcctc gcatccaccg ttggcagcgt ccgtgcgatc cttcgaacgg acttcagcgc
    11521 ggaaagtgta ctaccagcgg ctaggggggg ccagcggcca acagggaaat cgggatcacc
    11581 agatttcagc ccggcttcga ccagcaccgg tgccccggct ccgctatggc tgcgccgcgc
    11641 ggcgcagata ctcaaccagc aattgaacgc cgaagcctgt cgcgcccttg ccggtcggcg
    11701 aattcgactc ctccagcgtt gccaccgccg cgatgtccac atgcgcccac gccattcccc
    11761 tggcgatgaa ttccttgagg aacatcccac ccacgatcgc ctgtgcctcg cgcttgccgg
    11821 atgagttacg gatatccgcg tccgttccct tgatcagctc acggtactcg tcccacagcg
    11881 gcagccgcca gagccgttca tgtaccgccc ggccgcactc gccaagcacg ccggccagtt
    11941 cgtcatcggt gctcatgata ccggccgcga ccttgcccag cgccaccgtg accccgccgg
    12001 taagcgtagc gatgtcgatg atttcggtgg ggttgaggtg ctgctgtgcg taccacagcg
    12061 cgtcggccag caccagccgc ccctcggcat ccgtgctgat tacttccacc gttttgccgc
    12121 tggccatcgt cagaatgtcg ccgggacgat aagcccgctc ggagattgcg ttttcggccg
    12181 ctccgatcac gcccgctacg ttgcacggca gccccaactg cgacgccgcc tgcaaggcgc
    12241 ccaatacggc cgcgccgccg cacttatcga atttgagccc ctccagcccg gccgccggct
    12301 tgatggaata gccgcccgtg tcgaaggtga tcgccttgcc gacaagaacc gtgttgcggc
    12361 gcgaatcggg gcgcggccgg tactcgagct gaatcaggca tggtgcgtga gccgaaccgc
    12421 cgccgaccgc caggagtccg cccataccca tgcgccgcgc ctcggcggcg tcgacgagct
    12481 tgtatttcaa gccgcagtgg cgcgccaggc gcttggcttc ttccgccaga gtctgcggat
    12541 tgatgacgtt ccccggcaag tgagccaagc ggcgggcgta attgacggcg gcggcgatcg
    12601 tcgtcgcagt cttgatttcg cccattgccc tgcggaccag ccccggatcc gcggcacgga
    12661 gctggaggcg cactttcttg ggcgttctgt tgttgttctt caggaattcg gtgaagcgaa
    12721 agccggctag cgccatcccc agcgtccact ccctgaagca ttcattcagc ccggttgagg
    12781 ccagtccatc cagccagacg gcgccgctgg taagcttctc tgcaatcagc caacgggccg
    12841 cggaggcggc ggcctggcgc acgaccaagc ccgtcagctt ctccgcgggg ccgagatcta
    12901 cgacaagaat tcgggggacc ggcccggcgc tggtggtgtg cgccaagtgc ccggcttcgg
    12961 cgcccacggc ctgcactgcg agtaatgcgg ataccgcgtc gccgcaagcg tcgtccacgg
    13021 ccttcaccag cggcaacggc gggggcggct ttgtcaccaa ggggaccaac aggacgtcag
    13081 acggcgtgcg gggcttctgc ccggccacaa actcgaattc agttccgttt ggctctgccg
    13141 gcatgcgcaa actccgattg cgtactccct tatttggagc ccatgagatt agcatgaggg
    13201 gcttttgggc gaaacgggtc ggcgacaaca catcgcttcg gcgatttgct ggccgctgga
    13261 tttgtccctg gattgcggag cgcttctatc tgcgtatcga ccgcagaaac tcctgcacct
    13321 cggggatttc gccctggtag atcagatacc cgttgtacgg ctcgcgcagc gtgatctcgt
    13381 cgataatcgg cgtgagcatg atgcgtttga cctcttcgcg gtcgtggctc tgccccaggg
    13441 cccagcacaa cttcatgtag gccacctcgg gcagcatgtt ggccgccggc acaacgccca
    13501 gctccatcag gtcgcgcccg gtgctgtata cgtacatctg cacgtagccc cacagcgtct
    13561 gcacggtcat gtagacgtgc acgccggcct cgatggcccg cctcagggcc ggatagaggg
    13621 gcttgttcac gtgccccagg ccggttcccg cgatgataat tcctcggtag cccttctcca
    13681 ctaccgcgtc gaccaggtcg ggctgc
//
LOCUS       DAOSYS010000078        13696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3784_length_13696_cov_5.047577, whole genome shotgun sequence.
ACCESSION   DAOSYS010000078 DAOSYS010000000
VERSION     DAOSYS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3784_length_13696_cov_5.047577"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLU99_06545"
     CDS             complement(<1..485)
                     /locus_tag="PLU99_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO16753.1"
                     /translation="MSETISEKAKVHLRWALIDGVGPLTFSHLVSYFGDAAQALSASA
                     TQLEQVPRIGPKLAEAIVRTRDHVALDEEIEAAAAHGVRIICRDDPEYPPGLRQIPDA
                     PIVLYIKGEYRQTDTLALAVVGSRRCTIYGSEQARRFGELLAGAGFTVVSGLARGIDS
                     FA"
     gene            644..901
                     /locus_tag="PLU99_06550"
     CDS             644..901
                     /locus_tag="PLU99_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16754.1"
                     /translation="MATDPIKTHRAGRNLGLWGATFAAVLAMSICTGCNDSVRREVRQ
                     GAYEVFSAGLDVFYSELGAGITNGISELGQAASDASAAKGS"
     gene            complement(957..2441)
                     /gene="rpoN"
                     /locus_tag="PLU99_06555"
     CDS             complement(957..2441)
                     /gene="rpoN"
                     /locus_tag="PLU99_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOO16755.1"
                     /translation="MSQILSQVPSQLLQQQQRLTPQLIQAMDILQLPMMALESRIAQE
                     IDGNPALELVLPEDEAAEAASAAETEDEPPSGEKALVVEEGNAAEFERLDNLVREYDW
                     IEDDAEYRGTKSRARSLEEGDIKMEAMANTAARPVSLQEYLSQQWSLTEVDEQTRQIG
                     AKIIDFVDENGRLSTPLEEIAREFVEPPSLDQMEQALRAVQRRAPPGIAARNLRECLL
                     LQLEALPGDTSLERKIIERHLEDLQKNRLPQIARALGVDLSDVRGALGVITRLSLRPG
                     LKVVSQPAPPIVPDVIVDYNEKENQYEVRLARGNARELRISPEFREALEKTRADKRSR
                     DFIKQKIEAANAIIDAVRYRRERLLEVAKAVVEAQRDFLDYGEQHLKVLRMSDLAARF
                     GCDPSTISRTVDEKYMQTPRGVFPLRRFFTGGTESGNGEVLGWDSIKAKVQEIVAKED
                     KRNPLSDDEIVSKLKLLGIEIKRRTVAKYRAQLEIPTARQRRQY"
     gene            2667..3923
                     /gene="icd"
                     /locus_tag="PLU99_06560"
     CDS             2667..3923
                     /gene="icd"
                     /locus_tag="PLU99_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOO16756.1"
                     /translation="MPLRTTEVPPDGGKIRKIDAKLSVPDTPIIPFIRGDGTGPDIWR
                     ASQMVFDAAVSRAYGSRRRIAWMELFAGEAAKQRFDDWLPAETLEAFREFLVGIKGPL
                     TTPVGGGIRSLNVALRQQLDLYVCLRPVRYFPGVPSPIRKPELTDMVIFRENSEDIYA
                     GIEFQQGTPECRRFVELFAENFPDRYAKIRFPETSGIGIKPVSRQGTERLVRAAIRYA
                     LENGRRSVTFVHKGNIMKFTEGAFREWGYALAKREFGAVDFEGGPWQVIPRDSYPLLI
                     KDAIADAFLQQILTRPVDYDVIATMNLNGDYISDALAACVGGIGIAPGGNINYETGHA
                     IFEATHGTAPKYADQDKVNPGSVILSGEMMFRYIGWTEAADLIIRGIERTIAAKTVTY
                     DLERLMEGATLLKTSEFGRAIVANMD"
     gene            4177..4653
                     /locus_tag="PLU99_06565"
     CDS             4177..4653
                     /locus_tag="PLU99_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16757.1"
                     /translation="MKTTLIVVVVVALLEGGAFFGAMKFFGGGPAPTYGADGNHAIEG
                     PPPPPEKGSAELMLLKNFRVPNIKSGRTVFYDFDISVAVPEDRKAEVEELAKSRDAEI
                     RDRVSQIIRQARPAVLEEDDFATLRMLLSRALGEVLGDTELIQRVLIPRCLPLPGD"
     gene            4778..5359
                     /gene="fliN"
                     /locus_tag="PLU99_06570"
     CDS             4778..5359
                     /gene="fliN"
                     /locus_tag="PLU99_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOO16758.1"
                     /translation="MAETPQSPTPQSRDPNLIDQAELDRLTQALEAEATARPTEPSSE
                     APAPPEQLDATPPADETATPTENPDTTPAEGPAVIGSTAPVNSTEAAPLEMTEFAEPS
                     DQINLANIDLLDDVELDVKIELGRTQMYIEDVLRLGEGSVVELDKLAGDPVDVYVNDR
                     LVARGEVLVLNDNFCVRINDIISVNPELEEMRK"
     gene            5362..6090
                     /locus_tag="PLU99_06575"
     CDS             5362..6090
                     /locus_tag="PLU99_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOO16759.1"
                     /translation="MNRSPWMALICLGSLTTNGLALAPSRPAAKPALRAFPAESGAPA
                     AVEPDPWDSPEIAAPQLEDDLVRRPSAARGTNSRSSQGSGTVGSLVRTSASLAGVVAL
                     IVLLAWGYRAASGAGNRFGSRWRGAEPGLIQVLHRTSIGPRQGLCLVRVGPQLVLLGT
                     THDSIRALTVVQDADLAARVAGQRPQANGQRAEFQACLETESRAYTEPAPATAGQDAP
                     GLGEVKHKLSQTLRRMEKLAAPGA"
     gene            6134..7051
                     /gene="fliP"
                     /locus_tag="PLU99_06580"
     CDS             6134..7051
                     /gene="fliP"
                     /locus_tag="PLU99_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437724.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOO16760.1"
                     /translation="MNHAARPAHPTPEHTSAARGAAPEVDGNSGFRPDRRALWIYLLL
                     PLAIALAVPLAAQDPATAIPAAPASDPVYIPDVSQLLPPAKSKESLSATMQILVLMTL
                     LTVAPSILLMMTSFARMVIVLVLLRQALGTQQLPPSQVMIGLALFLTFLVMAPTWERV
                     RANAIDPYLDGRLGQLDAVSAASSELRHFMFTQIEAANNEEDVYLMYEYATRQPVPAE
                     VALQRSTVPMSALIPAFILSELKTSFILGFRIYLPFLVIDMVIATVLVSMGMMMLPPV
                     LISLPFKLLLFVLADGWHLIAGALMASVY"
     gene            7120..7374
                     /locus_tag="PLU99_06585"
     CDS             7120..7374
                     /locus_tag="PLU99_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HOO16761.1"
                     /translation="MDMTAALDLGREALLVSLIIAAPVLGVGVVVGLVISVIQTVTQL
                     NDQTLALVPKIVAMVAATMLLVPWLATRLIDYAQAMFAGI"
     assembly_gap    7507..7606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7607..7860
                     /locus_tag="PLU99_06590"
     CDS             <7607..7860
                     /locus_tag="PLU99_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOO16762.1"
                     /translation="LTVASATAIRIAAPAVLTLFLVNLAMGFIARTVPQLNVVTVGFS
                     IKALIGFAIMAVALPSAMNAFVSALDISLEWLHELIGTM"
     gene            7880..8947
                     /gene="flhB"
                     /locus_tag="PLU99_06595"
     CDS             7880..8947
                     /gene="flhB"
                     /locus_tag="PLU99_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOO16763.1"
                     /translation="MALDDGGDRTEAPTPRRLQEARDEGRIPRSVDLTAAVALMAAAI
                     LLKTLGSGLFGSLLQLTSDLGEPSDVAGGDLAAVVLRVALATARGLLPFLGLLLLATA
                     LGAVVQSGLSPSLKPLEPKLEKLNPAAGVRRLFSLDSATRAGQGVFKITLVALVAYVT
                     ITGELDTVLNAGLLEPAGIVQMSAALLYKLALRLGLTLLVLALLDYWYQRWNWWRRLK
                     MTKQEVRDELKRMEGDPQMRHRRRQLQMRLALQRVGIDVPKADVVVTNPTEYAVALKY
                     DEATMTAPRVMAKGKDLLAARIRQVAQQHRIPIVQRPPLARALYAAVEVGQEVPPAYY
                     RAVAEVLAYVYRITGKLRAAG"
     gene            8968..11082
                     /gene="flhA"
                     /locus_tag="PLU99_06600"
     CDS             8968..11082
                     /gene="flhA"
                     /locus_tag="PLU99_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOO16764.1"
                     /translation="MLDNHPITLFIARHRGLLFPCAAAALIFVILIPLPTWVLDFLLI
                     SDVLLATIVLMTVMYLRAPLEFSSFPSLLLAATLFRLVLNTATTRLILTNGQAGTDAA
                     GQVIYTFGTFIAGNSLAVGVIIFLIITVVQFVVITKGATRIAEVAARFTLDGMPGKQM
                     AIDADLNAGLIDETEARRRRRQITDEADFYGAMDGASKFVRGDAIAGLVITLVNILGG
                     LYVGMVEGGMGVMNCLSVYTKLSIGDSLAAQIPAFLLSVAAAMLVTRSTGQTNMGEEV
                     IGQLASRPIALLGAAAFLGVLMLTPMPKVPLLTMAGGCGTLAWFIRQHQVSQANRLAG
                     EQRAKERTKPQQIEAHLAVDALELQIGFGLVKLVDRARGGDTLDRIAALRKQMAIDLG
                     LIVPPIRIRDNSEIAPNRYLVLLRGQEIAGGELFPDQVLAIDSGLAGQRLSGMETREP
                     AFGLKAWWIQPDDRERAESLNYTVVEPTGVLATHLTELIKRHAAELLTRADTQRLIDA
                     LKQRNATVVEEVVPNVLKVGEVQRILQNLLRERVPVRDLEAILEALGDWAPKSKDPEI
                     LTEYARNALARTICSQYKDAHGVIHCVTLDPASEDYLAANIQRVDSGSVLLLPPERQS
                     EIATRTREVIEAAGPAAAGATIVLLCSPQVRVWLRRIIEAVLPQTPVLALNEIARGID
                     VQAHGVVSFGSQPADIQSTVHA"
     gene            11075..12265
                     /locus_tag="PLU99_06605"
     CDS             11075..12265
                     /locus_tag="PLU99_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16765.1"
                     /translation="MPEALALVKRELGPEAVILGTRTLPAHGLSRLVGRDQVEITAAL
                     ETSLAPTTRTMPKAGTMPKAGAMPKPALRACPTGPVVEMCEGAKTGQREPETQTPKLE
                     TQLSEVKTRPRTCPAAKPETLDSSLEPVPPTIPEEVYPYYVRLVQQELGRELAQRVLA
                     SALERLPRGTRPHPTKLNELLRETLTGMIPTAADGEVPAARRIAFVGPPGGGKTTTLA
                     KLAAHLHLRQKKSVALLSLDMHRLAAGEQLRRYAELIQVPIAVAQTREGVAQALRDFR
                     DIDHILIDTPGVGLREEGRFARMAALLRATRPDEVHLVLPGSTSPAVQLRLGQAFAPL
                     GISRLAVTRLDETIGFGVILTLLEQLQLKLSYFSTGQNVPQDFEAACGRRVAELICPP
                     QVGA"
     gene            12616..12912
                     /locus_tag="PLU99_06610"
     CDS             12616..12912
                     /locus_tag="PLU99_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16766.1"
                     /translation="MERSIGAQVGLLAFAAAIVAGLYVGNAPTTILVRALIVMLAACA
                     AGQAVGWAVKQVLRDHLQQKKQAIDREHYEALHGADGQSPAQPVPAESQPARAG"
     gene            12918..>13696
                     /locus_tag="PLU99_06615"
     CDS             12918..>13696
                     /locus_tag="PLU99_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOO16767.1"
                     /translation="MPRRIGSSRSRRVSREHRVRTFPNDAALLSAWRRYRATGDETLR
                     NRLMEHYLYLVKYNAERIGAKLPDEVDVDDLMSAGVFGLLDAIDAFDLDRGVKFETYC
                     SPRIRGAILDELRNMDWVPRLVRHRAHKLADITRTLEVELGRLPSEDEIANRLGMTRP
                     QFDKMMRDASTVSLISISRKFANSDSDRDVFEIDLVPDHQSSDPVIEAQKRDVRDLVT
                     RGLNRAERLIVVLYYYEQMTMKQIGETLDLSESRVSQMHSA"
BASE COUNT         2476 a         4530 c         4105 g         2485 t
ORIGIN      
        1 gcgaacgaat cgattccgcg cgccagtccg ctgaccaccg tgaaccccgc gccggccagc
       61 agttcgccga agcgccgggc ctgttcactg ccgtagatcg tgcagcgccg gctgccgacg
      121 accgccaggg ccagcgtgtc ggtctggcgg tattcacctt tgatatagag cacgatgggc
      181 gcgtccggaa tttgccgcag tccgggcgga tattccggat cgtcgcggca gatgatgcgt
      241 acgccgtgcg cggccgccgc ctcgatctct tcatccagcg cgacgtggtc gcgcgtccgc
      301 acgatggcct cggccaactt cggcccgatg cgcggaacct gctcgagctg cgtggcgctg
      361 gcggaaagcg cttgcgccgc atcgccgaaa taggaaacca ggtgcgaaaa ggtcagcggc
      421 ccgacgccgt cgatcagggc ccagcgcaag tgaactttgg ctttttctga gatggtctcg
      481 ctcattccga tcgcccgagc agaagcgctg attctacacg ccagacgcga caatggaact
      541 ggtgacaaat ggcacagcgg ttgcattttc cgactgcgga tgccggggtc tgcggaccgc
      601 gcagccgatt agacgcgtgg attgctgtaa aacggagctt gccatggcaa ccgatccgat
      661 taagactcat cgcgcggggc gcaatttggg cctgtggggc gcaacgtttg cggctgttct
      721 ggccatgagc atttgcaccg gctgcaacga ctcagttcgg cgtgaagtac gccagggcgc
      781 ttacgaggtt ttcagcgcgg gcctggacgt cttctactca gaattggggg ccggcataac
      841 taacggaatc agcgagctgg gccaggcggc ttcggacgcg agcgcggcga agggaagtta
      901 agcgtttagc gccggcaacc actttcgccc gtctcaaatt ccgctttaca cggcgtttag
      961 tactgccgcc gctgccgcgc cgtgggtatt tcgagctggg cccggtactt ggcgaccgtg
     1021 cgacgcttga tctcgatccc cagcagcttc agttttgaga cgatctcgtc atcactgagc
     1081 ggattccgtt tgtcttcctt ggcgacgatt tcctggacct tcgctttgat gctgtcccac
     1141 cccagaactt cgccgttgcc cgactccgtc ccgcccgtga aaaaccgccg caacggaaaa
     1201 acgccgcgcg gcgtctgcat gtacttttcg tccactgtgc ggctgatggt cgaaggatcg
     1261 cagccgaagc gcgccgccag atcgctcatt cgcagcactt tcaggtgttg ctcgccgtag
     1321 tccagaaaat cgcgttgagc ctccaccacc gccttggcca cttccaggag ccgctcgcgg
     1381 cggtagcgca ccgcatcaat gatcgcgttg gccgcctcga tcttctgttt aatgaaatcg
     1441 cgcgaccgct tgtccgcccg cgtcttctcc agtgcctcgc gaaattccgg cgaaatccgt
     1501 agctcgcgtg cattgccccg cgccagccgc acctcgtact gattttcctt ctcgttgtaa
     1561 tccacgatca cgtcgggcac gatcggcggc gccggctggc tcaccacttt cagccccggg
     1621 cgcagcgaca gccgcgtaat gacgcccagc gccccgcgca cgtccgacag gtcgaccccc
     1681 agggcccgcg caatctgcgg cagccggttc ttttgcagat cctccagatg ccgttcgata
     1741 atcttccgct ccaggctggt atcgcctggc aaggcctcca gttgcagcag caggcattcg
     1801 cgcaggttgc gcgctgcaat ccccggcggc gcacggcgtt gcactgctcg cagggcctgt
     1861 tccatctggt cgaggctggg cggttcgacg aattcgcgcg cgatttcctc cagcggtgtg
     1921 ctcagacgcc cgttctcatc aacgaaatca atgattttgg cgccaatctg ccgcgtctgc
     1981 tcgtcaactt cggtcagact ccattgctgg ctcaggtact cctggaggct gaccgggcgc
     2041 gccgcggtgt tcgccatcgc ctccatcttg atgtccccct cttccaagct gcgcgcccgc
     2101 gacttggttc cgcggtattc cgcgtcgtcc tcgatccagt cgtactcccg aaccaggttg
     2161 tccagtcgct cgaattcggc cgcgttccct tcctcaacca ccagcgcctt ttcgccggag
     2221 ggcggctcgt cctcagtctc tgccgccgac gcggcctcag ccgcctcgtc ttccggcagc
     2281 accagctcca gcgccggatt tccgtcaatt tcctgtgcaa tgcgcgattc cagggccatc
     2341 atgggcaatt gcagaatgtc cattgcctgg atgagttgcg gcgtcaggcg ctgctgctgc
     2401 tggagaagct ggctaggaac ttgcgaaaga atttgcgaca tgctcttcgg ttctgaactc
     2461 ctaggacgta ccctggcgcg ggcacacacg ggtcggatgc ccgacgttct cagggcaaat
     2521 cactacaata agaatagtca tcggaccgcc gcgcgtcaaa agaatcatga cgcttggcac
     2581 agcgattgcc tgtcgggccg cccccgtgct acgatagagc gcttttgcgg cggtctgacg
     2641 gagttgcgtc gtccgaggag agctgcatgc cgttgcgaac tactgaagtc ccgcccgatg
     2701 gtggcaagat caggaagatt gacgccaagc tgtctgtccc ggacacgccg ataattccct
     2761 tcattcgagg cgacggcacc ggtccagaca tttggcgcgc gtcccagatg gttttcgatg
     2821 ccgccgtcag ccgcgcctac ggctcgcgcc gccgcatcgc ctggatggag ctgttcgcgg
     2881 gagaggctgc caagcagcgt ttcgacgatt ggctgcccgc ggagacgctc gaggcttttc
     2941 gtgagtttct ggtcgggata aaaggcccgc tgaccacgcc cgtgggcggc ggcatccgct
     3001 cgcttaacgt ggcccttcgc cagcaactcg acctgtacgt ttgcctgcgc ccggtccgct
     3061 attttcccgg cgttcccagc cccatacgaa aaccagagct gaccgacatg gtcatcttcc
     3121 gtgagaattc ggaggacatc tacgccggaa tcgaattcca gcagggcacg ccggagtgcc
     3181 ggcgcttcgt ggagcttttc gccgagaact tccccgaccg ctacgccaag attcgcttcc
     3241 ccgaaaccag cggcatcggc atcaaacccg tcagccggca gggcacggaa cgcctggtgc
     3301 gggcggccat ccggtacgcg ctcgagaacg gccgccggag cgtgactttc gtccacaagg
     3361 gcaacatcat gaaattcacg gagggggcct tccgtgaatg gggctatgcc ttggccaagc
     3421 gcgagtttgg cgcggtcgat tttgagggcg gcccctggca ggtgattcct cgcgactctt
     3481 acccgttgct gataaaggat gccatcgccg acgccttcct acagcaaatc cttacgcgcc
     3541 cggtcgatta cgacgttatc gccacaatga acctgaatgg cgactacatc tccgacgcgc
     3601 tcgccgcctg cgtcggcggc atcggaattg cgcccggcgg caacatcaat tacgaaaccg
     3661 gtcatgccat attcgaagcc acccacggca ccgctccgaa gtatgccgac caggacaagg
     3721 tcaaccccgg ttcggtcatc ctcagcgggg agatgatgtt ccggtacatt ggatggaccg
     3781 aggcggcgga tctcatcatt cgcggcatag aacgcacgat cgccgccaag actgtcactt
     3841 acgacctgga gcgcctcatg gaaggcgcta ccttgcttaa aacctcagag ttcggtcgcg
     3901 ccatcgtcgc gaatatggac tgaatcgcag gcgagcgctg cccaagctgg acccccggac
     3961 gcgcagactt tgttgcgccc tcagcgcaat cgctgccgcc agtgccgcct atgtctcata
     4021 agagtcccgt tcgcatcgcg ccgcgcccgc ggcaatgaac cccctcggcc gtcccgtcga
     4081 taacgacgct gaaggggcgc ccgcgcgggc ccccggaggt cctgtatcgt gtccgaagag
     4141 aacctggctg cagcgccgcc gcccaagggt ggttcaatga aaacgaccct gatcgttgtg
     4201 gtcgtcgtcg ctctgctgga gggcggcgcc ttctttggcg ccatgaagtt cttcggcggc
     4261 ggaccagccc ccacctacgg cgccgacggc aaccacgcca tcgagggtcc gcccccgccg
     4321 cctgaaaagg gctcggccga gctaatgctg ctgaagaact ttcgcgtgcc aaacattaag
     4381 tccgggcgca cggttttcta tgacttcgat atcagcgtgg ccgtcccgga agatcgcaag
     4441 gcggaagttg aggaacttgc gaagagccgc gacgcggaaa ttcgcgatcg cgtatcccag
     4501 atcattcgtc aggctcgccc ggccgtctta gaggaagacg actttgcgac cctgcgcatg
     4561 cttctaagcc gcgccctggg cgaagttctg ggagacaccg agttgattca gcgcgtactg
     4621 attccgcgct gccttccgct cccgggcgat taagcagagc cgggctgtgc cagcccggag
     4681 cgtagcaaag cggagccgca ctgtatcagc ccggagcata ccaggcgctt gagccaacga
     4741 accgccagga tggcgaaact ttcacaggat gtgaaccatg gccgaaacgc ctcagagccc
     4801 aacgcctcag agccgtgacc cgaatctgat cgaccaagcc gaactcgacc gcctgacgca
     4861 ggcgctcgaa gcggaggcca cggcgcggcc gactgaaccg tcgtccgagg cgccggcacc
     4921 gcccgaacag ctcgatgcta caccaccggc ggacgaaaca gctacgccaa ccgaaaaccc
     4981 ggacacaacg cctgccgaag ggccggccgt gatcggttca accgcacccg tgaattcgac
     5041 cgaggcggct ccgcttgaaa tgaccgaatt cgcggagccc agcgatcaaa tcaacctggc
     5101 aaacatcgac ttattggacg acgtcgaact ggatgtgaag atcgagctgg gtcgcacgca
     5161 aatgtacatc gaggatgtat tgcggctggg cgaaggctcg gtggtggaat tggacaagct
     5221 ggccggcgat cccgtcgacg tgtacgtcaa tgaccgcctg gtggcgcgcg gcgaggtgct
     5281 ggtgctgaac gacaacttct gcgtccgcat caacgacatc atcagcgtca atcccgaact
     5341 cgaagaaatg cggaagtaat gatgaaccgt tcgccatgga tggcgttgat ctgcctcggc
     5401 agcctgacca ctaacggtct ggcgctggcg ccgagccggc ccgcggcaaa accggcgctg
     5461 cgggccttcc cggctgaaag tggggcgcct gcggcggttg agccggaccc gtgggactcg
     5521 ccggagattg cggcgccgca gcttgaagac gatctggttc gcagaccgag cgccgcccgc
     5581 ggcacaaatt cgcgttcgag ccagggcagc ggcaccgtcg gcagcctggt gcgaacctcg
     5641 gcgtctctgg ccggcgtcgt tgcgctcatc gtgctgctgg cttggggata ccgggcagca
     5701 tccggggccg gcaaccgttt tggatcgcgc tggcgcggag ctgagccggg tttgatccag
     5761 gtgctgcacc gcacgagcat cgggccgcgg caggggctgt gcctggttcg cgtcggcccg
     5821 caacttgtct tgctgggtac tactcacgat tcaattcggg ccctgacggt cgtccaggat
     5881 gcggacctgg cggcccgtgt cgccggtcag cggccgcagg cgaacggaca gcgcgccgaa
     5941 ttccaggctt gtctcgaaac agaatcacgc gcttacaccg agcccgcgcc ggcgaccgcc
     6001 gggcaggatg cgccgggact cggcgaggtg aagcacaaat tgtcacaaac actgcggcga
     6061 atggaaaaac tcgccgcccc aggggcctga cggcaggaaa ctcattcaca cgagtgggac
     6121 gagggatcag gcgatgaacc acgcagctcg gccagcacat ccgaccccgg agcacaccag
     6181 tgctgcgcgg ggcgctgcgc ctgaggttga tggcaacagc gggttccggc ctgaccggcg
     6241 ggcactgtgg atctatttgc tcctgcccct ggcgattgct ctcgccgtac ctttggcggc
     6301 ccaagacccg gcgactgcaa taccggcggc cccggccagt gacccggttt acatacccga
     6361 cgtgagtcaa ctgctgccgc ccgccaagtc caaggagtcg ctcagcgcca cgatgcaaat
     6421 tctggtgctg atgacgttac tgacggtggc gcccagcatc ctgctgatga tgacctcttt
     6481 tgcgcggatg gtcatcgtgc tggtcctgct gcgtcaggcg ctgggcacgc agcagttgcc
     6541 gccgtcgcag gtgatgatcg ggctggccct ctttctgacc tttttggtga tggcgccgac
     6601 ttgggagcgc gtgcgcgcca acgccatcga tccttacctg gacggccgcc tgggccaact
     6661 cgacgccgtc agcgccgcca gcagtgaact gcggcatttc atgttcactc agatcgaagc
     6721 ggccaacaat gaagaagacg tctacctgat gtacgagtac gccacgcggc agccggtgcc
     6781 tgcggaagtt gccttgcagc gcagcaccgt gccgatgtcc gcgctgatcc ccgcttttat
     6841 cctcagcgag ctgaaaacct cgttcatcct gggatttcgc atttacctgc cgttcctggt
     6901 aatcgatatg gtgatcgcca ccgtgctggt ttcaatgggc atgatgatgc tgccgccggt
     6961 gctgatctca ttaccgttca agttattgct ctttgtgctc gccgacggct ggcacctgat
     7021 agccggcgca ctcatggcca gcgtgtattg agggcccagt cagatgcgct ttcgtgacct
     7081 tttctctaga tactgcggtc tctcttccca ggagctgtca tggacatgac ggcggcgctg
     7141 gacttgggcc gtgaggcact gctggtgtcc ctcatcatcg ccgcgccggt gctcggcgta
     7201 ggcgtcgttg tcggcctggt gatcagcgtc atccagaccg tgacgcaatt gaacgaccag
     7261 actctggcat tggtgccgaa gattgtagcc atggtggccg cgaccatgct gctcgtaccg
     7321 tggctggcaa cgcgcctgat tgactacgcc caggcgatgt tcgcaggaat atgagacttt
     7381 taccacataa cgtatgccct acgaactgct gtcctggtat ttgaagctgc ctctgttcgc
     7441 gctggtgctg gcgcggctcg ccggcatgat cgcctggcaa ccagtgctct ccgccttggc
     7501 ggtcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncatt gacggtcgcc
     7621 agcgccaccg ccatccgcat cgcggccccg gccgtactca ccttgtttct ggtcaacctg
     7681 gcaatggggt tcatcgctcg cacggtgccg caactgaacg tggtgacggt gggcttttcg
     7741 ataaaggcgc tgattggatt cgccatcatg gcggtcgccc tgccttccgc catgaacgcc
     7801 tttgtcagtg ccctagatat cagcctggag tggctgcacg aactaattgg cactatgtag
     7861 ttgaagggac agcggctgaa tggctctgga cgacggcggc gacaggactg aagcaccgac
     7921 cccgcgtcgc ctgcaagagg cgcgcgacga gggccgcatt ccccgcagtg tggacctgac
     7981 cgccgcggtc gccctcatgg ccgcagccat cctgttgaag acacttggca gcggactgtt
     8041 cggcagcttg ctgcaattga ccagcgacct cggcgaacca tcggacgtag ccggaggcga
     8101 cctcgccgcg gtggtgctgc gcgtcgcgct cgcgactgca cgcggcctgc tgcccttcct
     8161 gggactattg ctgctggcca cggcgcttgg cgcggtggtt cagtcgggcc tttccccgtc
     8221 cctaaaaccc ctggagccca agctggagaa gctcaacccc gccgccggcg tacggcgcct
     8281 gttttcgctc gactcggcca cgcgcgccgg ccagggcgta ttcaaaatca ccctggtcgc
     8341 cctggtggcg tatgtcacca taaccggcga attggacacc gtgctgaacg ccggcctgct
     8401 cgaacccgcc ggcatagtac agatgagcgc cgcgctgcta tacaagctgg cgctgcgcct
     8461 ggggctgacg ctgctggtgc tggcgctgct ggattactgg tatcaacgct ggaactggtg
     8521 gcggcgcctc aaaatgacca agcaggaggt gcgcgacgaa ctcaagcgca tggagggcga
     8581 cccgcaaatg cgccaccggc gccgccagtt gcagatgcgc ctggccttgc agcgcgtcgg
     8641 tatagacgtc cccaaggcgg acgtggtcgt caccaacccc actgaatacg ccgtcgccct
     8701 gaaatacgat gaagcaacga tgaccgcgcc gcgcgtcatg gctaagggca aggacctgct
     8761 ggccgcacgc atccggcagg tggcgcagca gcaccgtatt cccattgtgc agcgcccgcc
     8821 gttggcccgg gcattgtacg cggcggtcga agtgggacag gaggtgccgc ccgcctacta
     8881 ccgtgccgtc gcggaagttt tggcctatgt ctacagaatc accggcaagt tgcgggccgc
     8941 cggataacta cgacgccgta ctgactgatg ctcgacaatc accccattac gctgttcatc
     9001 gcccgccacc gcggcctgct gtttccctgc gcggccgcgg cgctgatctt tgttattctc
     9061 atcccgctgc caacctgggt tctggatttc ctgctgatct cggacgtgct gctggccacg
     9121 atcgtcctga tgacggtcat gtatctccgc gccccgctgg agttctcctc gttcccctca
     9181 ctcctgctgg cggccaccct cttccgcctg gtactcaaca ccgcgaccac ccgcctgatt
     9241 ttgacgaacg gtcaggccgg cacggacgcc gccgggcaag tcatctacac gttcggcaca
     9301 tttatcgccg gcaactcgct ggcggtcggc gtcatcatct tcctcatcat caccgtggtg
     9361 cagttcgtcg tcatcaccaa aggcgccacg cgcatcgccg aagtcgctgc gcgcttcacg
     9421 ctcgacggca tgcccggcaa gcaaatggcc atcgacgccg acctcaacgc cggcctgatc
     9481 gatgagaccg aggcccgccg ccgccgccgt caaatcactg acgaggccga cttctacggc
     9541 gccatggacg gcgccagcaa gttcgttcgc ggcgacgcca tcgccggcct ggtaatcact
     9601 ctggtgaaca ttctcggcgg cctctacgtc ggcatggtcg aaggcggcat gggcgtgatg
     9661 aactgcctct cggtttacac caaactgtcc atcggcgaca gtttggccgc tcaaattccg
     9721 gccttcctcc tgtcggtcgc ggccgccatg ctcgtcacgc gcagcaccgg ccaaaccaac
     9781 atgggcgagg aagtcatcgg ccagctcgcc tcgaggccga tcgcgctgct cggcgccgcg
     9841 gccttcctgg gcgtactgat gcttacgcct atgccgaaag ttccgctgct caccatggcc
     9901 ggaggctgcg gcacactggc ctggttcata cgacaacacc aggtcagcca ggccaatcgc
     9961 ctcgccggcg aacagcgggc caaagagcgc accaaacccc agcagatcga agcgcacctg
    10021 gccgtcgacg cgctcgaact ccagatcggc ttcggtctgg tcaagctggt tgatcgggcg
    10081 cgcggcggcg acacgctcga ccgcatcgcc gcccttcgca agcagatggc gatcgacctc
    10141 ggcctgatcg tgccgcccat ccgcatccgc gataactccg aaatcgcgcc caaccggtac
    10201 ctagtgctgc tgcgcggcca ggaaatcgcc ggcggcgagc tcttccccga ccaggtgctc
    10261 gctatcgact ccgggctggc cgggcagcgc ctctcgggca tggaaacccg cgagccggcg
    10321 ttcggcctga aagcctggtg gattcagccg gacgatcgcg agcgcgccga aagcctcaac
    10381 tacactgtag ttgaacccac gggcgtgctc gccactcacc tgaccgagct gatcaagcgc
    10441 cacgcggccg agctgctgac gcgcgccgac acgcagcgct tgatcgacgc gttgaagcag
    10501 cgcaacgcga cggttgtaga ggaagtcgtg ccgaacgtgc tcaaagtggg cgaagtgcag
    10561 cgcattctgc agaacttgct gcgcgagcgc gtgccggtcc gcgacctcga agccatcctc
    10621 gaagcgctcg gcgattgggc cccgaagtcg aaagaccccg aaattctcac tgaatacgcg
    10681 cgcaacgccc tggcgcgcac catttgctcg caatacaaag acgcgcacgg cgtaattcac
    10741 tgtgtaacgc tggatccggc cagcgaggac tatctcgccg ccaatatcca gcgcgtcgac
    10801 agcggctccg tcctgctgct gccgcccgag cggcaatccg aaatcgccac tcgcacgcgc
    10861 gaggtcatcg aggccgccgg accggcggcg gccggcgcga caattgtgct gttgtgttca
    10921 ccccaggtgc gcgtctggct gcggcggatt attgaagccg tgctgccgca gacgcccgtt
    10981 ctggcgttga atgaaattgc gcgggggata gacgttcaag cccacggagt agtgagcttt
    11041 ggatcacaac ctgcggacat tcagagcacc gtccatgcct gaagccctcg cgctggttaa
    11101 gcgcgagctc gggcccgaag ctgtcatcct cggcacgcgc accctccccg cgcacggcct
    11161 cagccgcctc gtcgggcgcg accaggtgga aataacggcc gccctcgaga cctccctagc
    11221 gccgacaacg aggactatgc ccaaagcggg caccatgccc aaagcgggtg ccatgcccaa
    11281 gcccgcgttg cgggcatgcc caaccggacc agtcgtggaa atgtgcgaag gcgccaagac
    11341 gggacaacgg gaacccgaaa cccaaacccc aaaactcgaa actcaactct ctgaagtcaa
    11401 aaccagaccg cgaacatgcc cggccgccaa acccgaaact ctagactcga gcctcgaacc
    11461 ggtcccgccc accatccccg aagaagtcta cccctactac gtccggctcg tgcaacagga
    11521 actcggccgg gaattggccc aacgcgtact cgcgtccgcc ctggaacgcc tgccgcgcgg
    11581 cacgcgcccc catccaacca aactgaacga actgctgcgc gagacgctga ccggcatgat
    11641 cccgacggcc gcagacggcg aagtcccggc ggcgcgccgc atagcctttg tcggcccacc
    11701 gggcggcggt aagaccacga ctctggcgaa actggccgcc catctgcacc tgcgtcagaa
    11761 gaagtcagtc gcgctgctct cgttggacat gcaccgcctg gctgcgggcg agcagttgcg
    11821 gcgttacgcg gaactgatcc aggtcccgat cgccgtcgcc caaacccgcg aaggggtcgc
    11881 gcaggcgctc cgcgattttc gtgatatcga ccacatcctg atcgataccc cgggcgtcgg
    11941 cctgcgcgag gaaggccgct tcgcccgaat ggccgcccta ctgcgcgcca cgcgcccgga
    12001 cgaagttcac ctcgttctgc ccggctcgac ctcgcctgcc gtccaactgc ggctcgggca
    12061 ggcgttcgcc ccgctcggca tctcgcgcct ggcggttacg cgtctcgacg aaaccatcgg
    12121 cttcggagtt atcctgactt tgttggagca actgcagctc aagctgtcct atttttccac
    12181 cgggcaaaat gttccccagg acttcgaagc ggcttgcggg cgcagagtcg ccgagctaat
    12241 ctgcccccct caggttggcg cgtgatccaa ccagggaggc gagctcgtgg cgcgctacca
    12301 tccagcgcaa ccagccttgg tgtatggcaa accgccggca gttgaccgcc tgatcggact
    12361 ttcgccggcc cgccagggca cattccctct ccgtgatctc cgcgcgacac tagtccgggt
    12421 ggcggcgcag gccgccgccg ataaaccact gctcgccgta agtgcttccc cggtctgtac
    12481 ttggcgactc gctgcgcgcc cggccgccgg tcgcgccggc gattcgtacg ccgccggccc
    12541 ggggtcgaaa cgttcgatca aagcttcgtc caaagccgca tggaagcggc cgataggaaa
    12601 cacggacgtt accacatgga acgaagcatc ggagcacaag tcgggctgct ggcatttgcg
    12661 gccgcgatag tcgcggggct gtatgtcggc aatgccccca cgaccatact ggtccgggcg
    12721 ctgattgtta tgctggccgc ctgcgccgcc ggtcaggccg tcgggtgggc ggtcaaacaa
    12781 gtcctgcgcg accacttgca acaaaagaag caggccatcg accgcgaaca ttatgaggcc
    12841 ttgcacgggg ctgacggaca atcgcccgcc cagccggttc cggccgaatc acagcccgcc
    12901 agggcagggt gagcattatg ccgcgaagaa ttggcagcag tcgatcgcgc cgtgtcagtc
    12961 gcgaacaccg ggtgcgcact ttccccaacg atgccgccct gctcagcgcc tggcgccggt
    13021 atcgggctac aggcgacgaa actctccgca accgcctgat ggagcactat ctctacctcg
    13081 tcaagtacaa cgccgagcgc atcggcgcca agcttcctga tgaagtggac gttgacgacc
    13141 tgatgagcgc gggcgtcttc ggcttgctgg acgccatcga cgcattcgat ctcgatcgcg
    13201 gtgtgaagtt tgagacgtac tgctcgccgc gcatccgcgg cgccattctg gacgagctgc
    13261 gcaacatgga ttgggtgccg cgcctggtcc gccatagggc tcacaagcta gcggacatca
    13321 cgcgcacgct ggaagtcgag ctgggccgtc ttcccagcga ggatgaaatc gccaaccgcc
    13381 tgggcatgac ccgtccgcaa tttgacaaga tgatgcgcga cgccagcacc gtgtctctga
    13441 tttccatttc ccgcaaattc gccaacagcg actccgatcg cgacgtgttc gaaatcgacc
    13501 tcgtgcccga ccaccagagc agcgatcccg tcatcgaggc ccagaaacgc gacgtgcgcg
    13561 acctcgtcac gcgcggcctg aaccgcgccg aacggctgat cgtggtgctg tactactacg
    13621 aacaaatgac catgaagcag atcggcgaaa cgctcgatct ctcggaatca cgcgtcagcc
    13681 agatgcactc cgccat
//
LOCUS       DAOSYS010000079        13513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3844_length_13513_cov_5.666072, whole genome shotgun sequence.
ACCESSION   DAOSYS010000079 DAOSYS010000000
VERSION     DAOSYS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3844_length_13513_cov_5.666072"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLU99_06620"
     CDS             complement(<1..377)
                     /locus_tag="PLU99_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16768.1"
                     /translation="MEAERAALVQQRSEFDQARAAFERDQRAVNEAREACRKMEEELQ
                     ARAAEMTGQAAQLEQQRAELAVRQSELEATQAAQADWAAQLKELEARLGAREAALKSE
                     LNELLGRETALTKRAAELDAREAA"
     gene            complement(391..1491)
                     /locus_tag="PLU99_06625"
     CDS             complement(391..1491)
                     /locus_tag="PLU99_06625"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO16769.1"
                     /translation="MSSNVPNKPPGVPDGPPQAAAPWVRLQCDGEEGPTSRTVAASVV
                     LIGSRRDCDLPVTHPNVSKVHCALINTGRGILARDLISRCGTFLNDQPIRTAVLDAGG
                     VLRLGSVVVGFEFLAPSDGQAGPAHAARDDGPATVAIRGQILPLADNALVIGRRNTAD
                     VVVDTPDTSLAHALLFSLNGRPAIQDLGSRSGTWVDGERVELAWLSNGAEIGVGGERF
                     TVCWEEGAVEEESAVAAEQQAQAGGGKTASPAAPSVGQPPALENAPATLDELEQMVAA
                     VHSHLAAARGRLDERSRELDELAARVEAEKAEAQSRAAGLAEREAEVTARQAQLSQAE
                     QELAQRRAELEEQARQVSEERSALQAQRAEVE"
     gene            1639..1712
                     /locus_tag="PLU99_06630"
     tRNA            1639..1712
                     /locus_tag="PLU99_06630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1673..1675,aa:Val,seq:gac)
     gene            complement(1694..2326)
                     /locus_tag="PLU99_06635"
     CDS             complement(1694..2326)
                     /locus_tag="PLU99_06635"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HOO16770.1"
                     /translation="MLKYVVACVVAAGLCLAANQSRSSTETPRGAQPATRPAYTLDQL
                     AWLAGRWEGELSGERMEEHWSAAEGGLMMGMFRLMSRDGQTRMLELETLRQTATGVEL
                     RFRHFAPDLEPREEKGQTPTLILTELTEQRVVFEPPPAAEQRPGTPRRLIMLRSTPDL
                     YRVQVIVMRDGEEKLLFEAELRRVNAVSPGAVRAIFKGRAAIRENGRYWT"
     gene            2556..3746
                     /locus_tag="PLU99_06640"
     CDS             2556..3746
                     /locus_tag="PLU99_06640"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOO16771.1"
                     /translation="MNSPNEYVLAGGGTGGHLFPGLAVAAALRAIEPDAVVTFFTTTR
                     ALDRDLLARTPYGQVQQTVRPLTVRPWRIPAFLSAWFKNVAAASAWLKDHQPRAVLGL
                     GGYAAGPPVAAARRLGIRTAILNPDAIPGRANRFLASRVDLAVLQWDVTRRYLSKRTH
                     CHVLGCPIRAEFARPIDAGDARRSLGLDPNRPLLLVTGASQGARSVNQTVCRVWPEFQ
                     AKHPDWQLLHLTGAADEAETRRAYGPTLLNGTGTSEARGSVRVVAFMHEMWLALAAAD
                     LVVSRAGASTLAEITALGKPAILLPYPYHKDRHQHANAKVLVDAGAAVLIDDQKTAEA
                     NHRPLADALERLADGPTRQQMAQAARTLGRPAAARDVAAWLAGRRAAVDRLPIGAHNP
                     AHGG"
     gene            3736..4320
                     /gene="gmk"
                     /locus_tag="PLU99_06645"
     CDS             3736..4320
                     /gene="gmk"
                     /locus_tag="PLU99_06645"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006624538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOO16772.1"
                     /translation="MADKKRGKLVVISGPSGAGKSTICRELLRALPNSRCSVSATTRP
                     PREGEVDGRDYHFISRAEYDRMVAAGELLEHAEYCGQGYGTPRRAVEQALARGEIVIL
                     AIDIQGAAQIAQQAPESVRIFILPPTMETLQARLEGRQTESEAQVARRLATASHEIAH
                     ARASGLYHHFVTNDILEDSVQRVIEIIQQENDRE"
     gene            4317..4535
                     /locus_tag="PLU99_06650"
     CDS             4317..4535
                     /locus_tag="PLU99_06650"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOO16773.1"
                     /translation="MINPLQNDDLIRKAGGRFKLTALIQKRWLELMQGSRPLVDPTGK
                     TTMEIVIDEIVNDKIAIDYEASGIARPG"
     gene            4504..5163
                     /locus_tag="PLU99_06655"
     CDS             4504..5163
                     /locus_tag="PLU99_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOO16774.1"
                     /translation="MRPPALPARDNESQAGGNVPAGAANGQPLAGYEVLVCISGGIAA
                     YKTAVLVSQLVQLGCGVTVALTRNGRRFVGEVTFRGLTGRPVYTNASMWHTGEQIHMR
                     HLGLTEAADLVVVAPATANVIGKLASGIADDLVSTLLLGADSPVLLAPAMNERMWQHP
                     AVRRNVAWLREAGFLFVGPEEGWLACRAIGPGRMSEPEVLLAAIREQLLKAPAKRMGA
                     R"
     gene            complement(5343..6014)
                     /locus_tag="PLU99_06660"
     CDS             complement(5343..6014)
                     /locus_tag="PLU99_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16775.1"
                     /translation="MKVGIVVLGVFLLASAALADTISPCAVGPAAMSPAVRGTLGNPY
                     DEVQQYGSAAQDFETAYDAYDIWVVSDFTTYEDYYCETVTALGFTNGGVDGVGANFHI
                     YDGLPWAGGSIVMSASGGYDTLFSAGLIGADFGHQLLPAGSYYMVFQSVREFGTSGQS
                     YIYQTSLGNNNDYQWNPGAGFGFSTTPILDADSNPIDVNWQLVVTPEPSSLLLIAVAL
                     LLRRR"
     gene            complement(6566..6889)
                     /locus_tag="PLU99_06665"
     CDS             complement(6566..6889)
                     /locus_tag="PLU99_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO16776.1"
                     /translation="MTLSSSVRRRRRFQRAGGVACELPVLFTPVQWPVIAKVLRLTPR
                     QRAVAELLCAEYSQRDISQRLKVSANTVRTHLRALYARLGVRSRVGVVIRLIMAERES
                     WRCRQ"
     gene            complement(7037..7279)
                     /locus_tag="PLU99_06670"
     CDS             complement(7037..7279)
                     /locus_tag="PLU99_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16777.1"
                     /translation="MTRRDLERTLQECGEFAAARGEHSQADYFLTRLRDLRQSGQPGR
                     LPPSDTEDLLGQMRASLGSLSPSSQPAAESPARSSP"
     gene            complement(7315..9453)
                     /locus_tag="PLU99_06675"
     CDS             complement(7315..9453)
                     /locus_tag="PLU99_06675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO16778.1"
                     /translation="MTSDTNAGPTRQRRIVELFNAAVEMPPGQRDDFLAQACGGDLEL
                     QREIESLLACDTPAAVLEKPLLPSVRVADFCVPADQAPLPERIGAFEILNLIAVGGMG
                     VVYRAWQQTPPRTVALKLIRPDVMSREARRRFATEMETLALLRHPGIATIHEAGTVVT
                     PDGAQPYIAMEYVDGPPLLTFANEQPLSRRQRIELLARICEAVGHAHQRGVIHRDIKP
                     ANILVEQLDGIAQPRVIDFGVARVSGRDASATAVTVCGQVLGTLRYMSPEQIDGDPRA
                     VDTRSDVYSLGVVGYQLLSGALPVEVPESSFYAAARAVHDSSPLPLAARDRSLSGDLN
                     TIILKALGKNPDERYESAAALGADLRRYLAGEPILAQAPSTLYLLRRHLARHRMLVVA
                     AGIALLSLFVGLFGTGYGLMRARQSASALEDLSRQTIDAATFLVERTAKDLDRVAGTA
                     AVRRTLLERLDAQVDELLRREPEDEQLLTARAAVWARQAELLLSEKRHDEALTLRKRV
                     LELRRRLLASQPQSAQRGAEVSLTLVQIGDVMNALDQRNEARRLWEEALAMDEELVRQ
                     NPGVRRHLDHVAWGHDRLAAPLREGRLDEAEAHCRQRLTINEQLLAIEPDNLITYHGI
                     WEINQIICMLENAKADRHTQAEDIQASREAARAAAYAGLEAARHIVSRDPHNRRFLSA
                     EASALVRVAGQIRGTEPDEAARLYAAARCE"
     gene            complement(9450..10058)
                     /locus_tag="PLU99_06680"
     CDS             complement(9450..10058)
                     /locus_tag="PLU99_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HOO16779.1"
                     /translation="METALASQKSDNGDALTSDSSEASTADLGEAAGSGVALTADLAE
                     AVYSQLRSLAAACLRRERANHTLQTTALVNEAYLRLARRNVWPVNDRAAFCAAAAAAI
                     RRILTDHARRRRRLKRGGACVTMRLDDAAVAAAEPPIDVVQLDEALNRLRDESPRAAK
                     IVELRYFGGLTEQEVADVLGIARRTVQCDWRWARAWLMRELS"
     gene            complement(10283..10954)
                     /gene="nusG"
                     /locus_tag="PLU99_06685"
     CDS             complement(10283..10954)
                     /gene="nusG"
                     /locus_tag="PLU99_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOO16780.1"
                     /translation="MTEMPAKETPEEPPAEAREAAPAEAPATAAAVKAEPELVRPGMN
                     WYVLRVAANKEDRVREALERKIKIEQLEERVGRVLVPTQREKRIRGGSARVVHRKLYP
                     GYVFVEMATDEDGRIPERVWFVIKETTGVGDFIGSGGKPSPMPMHDVEKMLAAAIRPE
                     EGAALANLAFKKGDRVKVNEGPFENFEGVVDEINTQKGTVRVIVTIFGRATPIEIEYW
                     QVESV"
     gene            complement(10951..11457)
                     /gene="secE"
                     /locus_tag="PLU99_06690"
     CDS             complement(10951..11457)
                     /gene="secE"
                     /locus_tag="PLU99_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOO16781.1"
                     /translation="MADDSLSVEQPREPARPSEISRPAVSPLKIYKPGQGKHVRWGSA
                     IGAGVIAVAGVRFFYEWLRLPLGDNLVLRTLIPVAVLAALGWLIFWLVFQKHGTVDFM
                     IATEGEMKKVNWSSRKEVLGATKVVIFTVLALGFLLFIVDTLFMLAFSGMGVLKIPLW
                     KSWFGMAQ"
     gene            complement(11560..11633)
                     /locus_tag="PLU99_06695"
     tRNA            complement(11560..11633)
                     /locus_tag="PLU99_06695"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11597..11599),aa:Trp,seq:cca)
     gene            complement(11716..11883)
                     /gene="rpmG"
                     /locus_tag="PLU99_06700"
     CDS             complement(11716..11883)
                     /gene="rpmG"
                     /locus_tag="PLU99_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOO16782.1"
                     /translation="MAKAQKREWVWLQCKECSDLNYRTEVNVQGGVPKLELAKYCPRE
                     RKRTTHKIKRK"
     gene            complement(11961..13193)
                     /gene="tuf"
                     /locus_tag="PLU99_06705"
     CDS             complement(11961..13193)
                     /gene="tuf"
                     /locus_tag="PLU99_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOO16783.1"
                     /translation="MAKGVFERTKPHVNVGTIGHVDHGKTTLTAAMTAVQAARGLGVY
                     KSYDEIAKASEKDGRRDPTKILTIATAHVEYETEKRHYAHIDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHVLLARQVNVPALIVFLNKVDLVDDPELLELVEMEVR
                     ELLNKYEFPGDTIPVIRGSATEALKAAFDKNEAGYGPIQQLMDALDANIPEPVREQDK
                     PFLMPVEDVFSIKGRGTVGTGRIERGTVRVGDTVEIIGLHAPAGRQTVVTGVEMFNKT
                     LESGIAGDNVGLLLRGVEKKELERGQVLAKPKSITPHTKFMGEVYVLTKEEGGRHTPF
                     FPGYRPQFYFRTTDVTGSIIELQARDGGKAEMCMPGDNIQMRVEIHNPIAMEEGLRFA
                     IREGGRTVGSGVVTKILE"
     gene            complement(13319..13390)
                     /locus_tag="PLU99_06710"
     tRNA            complement(13319..13390)
                     /locus_tag="PLU99_06710"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13356..13358),aa:Thr,seq:ggt)
BASE COUNT         2438 a         4521 c         4074 g         2480 t
ORIGIN      
        1 gccgcttcgc gcgcgtccag ctccgccgcg cgcttggtca gcgccgtttc acgtcccagc
       61 agctcattta gctccgattt cagcgctgct tcacgtgccc ccaaccgcgc ctccagttct
      121 ttcaactgtg cggcccagtc agcctgtgcg gcctgagtag cctccagctc cgactgcctc
      181 accgctagct ccgcacgctg ttgttcaagc tgggcagctt gcccggtcat ctcggccgcg
      241 cgcgcctgca attcctcttc cattttccga caggcttcgc gggcctcatt gactgctcgc
      301 tggtcgcgct cgaatgccgc acgggcttga tcgaattcgg atcgttgttg caccagcgcc
      361 gcacgctcgg cttccattgc attgcggcca tcattctact tcggcgcgct gcgcttgcag
      421 cgccgaccgc tcttcagaca cctgccgcgc ctgctcttcc agttctgcgc ggcgttgcgc
      481 cagctcctgc tcggcctggc tgagttgtgc ctgccgcgcc gtaacctcgg cctctcgttc
      541 cgccagccca gccgcgcggg attgcgcctc agccttttcg gcttcgaccc gcgccgccag
      601 ttcgtccaac tcacgggaac gttcgtccag cctgccgcgc gccgccgcca gatggctatg
      661 aacggccgca accatttgct ccagttcatc gagcgtggcc ggagcgttct ctagtgctgg
      721 tggctgaccg acagacggcg ccgcggggga agcggtcttt ccgcctcccg cctgtgcctg
      781 ctgctccgcg gcgacggcac tctcctcctc gacggcgcct tcctcccaac ataccgtgaa
      841 tcgttcgcca ccgaccccga tctccgcccc gttcgacagc caggctaatt cgacgcgctc
      901 gccgtccacc cacgttcccg atcggctgcc gaggtcctgg atggccggcc gcccgttcag
      961 cgagaaaaga agggcgtgcg ccagtgacgt atccggcgtg tcgaccacca cgtcggccgt
     1021 gttgcggcgg ccgattacca gggcattgtc ggccagcggc aatatctggc cacggatggc
     1081 gacggtggcc ggtccgtcgt cgcgcgccgc atgagcaggg ccggcctgcc cgtccgacgg
     1141 cgcaaggaac tcaaagccca caacaaccga gcccagccgt aaaacgccgc cggcgtccag
     1201 caccgcggtg cggatgggtt gatcgttaag gaaggttcca caccggctga taaggtcgcg
     1261 tgccaggatg ccccgtccgg tgttgatcag cgcgcagtga accttggaga cgttgggatg
     1321 cgtgaccggc aggtcgcagt cgcggcggct gccgataagc acgacgctgg ccgcgacggt
     1381 gcgcgaggtc ggcccttctt cgccgtcaca ttgcaggcgc acccaaggtg cggctgcttg
     1441 aggcgggccg tcggggacgc caggcggttt attcgggacg ttggatgaca tcgagggccg
     1501 ctcacaggcc agggttttca ggttcctcag gaagtgttca aatcgtaggc aacgaacgca
     1561 aatcaaacgc cgccgcgcgt cacgggaacc gcttgacagc ttccgcctaa gcgacgatca
     1621 tcccttatgg gaacgcacgg gcgattagct cagttggcta gagcgcttgc tcgacatgca
     1681 agaggtcact ggttcaagtc cagtatcgcc cattctcgcg gattgccgca cgccccttaa
     1741 aaatcgcacg caccgcacca ggcgacaccg cattcacccg ccgcagctcc gcctcgaaca
     1801 gcagcttttc ctcgccgtcg cgcatgacga tcacctgcac ccggtacagg tcgggcgtgg
     1861 agcgcagcat gatgaggcgg cgcggcgtac cggggcgttg ctcagcagcg ggcggcggct
     1921 caaagaccac gcgctgctcc gtcagctcag tcaaaattag tgtcggcgtt tgccccttct
     1981 cctcgcgcgg ctcgagatcg ggcgcgaagt gccggaaccg aagctcgacc cccgtagccg
     2041 tctggcgcag cgtttccagt tcgagcatcc gtgtctgacc atcacggctc atcagccgaa
     2101 acatccccat catcaggccg ccctcggccg cggaccaatg ttcctccata cgctctcccg
     2161 acaactcacc ctcccagcgg ccggccagcc atgcgagttg gtcaagcgtg tacgccgggc
     2221 gcgtcgcggg ctgcgcgccg cgcggcgttt ccgtggacga gcgcgattga ttggcggcta
     2281 agcacaatcc ggccgcgacg acgcatgcaa caacgtactt caacatctcg gctctccagc
     2341 acggggtcgg cgggcactca agcgctccga agggcgccgt ccgaagatca ggaacatctg
     2401 cccaaactcg catgtacggc aaaaagtcgg aacatacctt accagttttc atccgaggtg
     2461 ccatttcagg ctcccgaaca ctcttgcagc cgtcgcgccg tccgttgcgc cgccgccggg
     2521 ttgccttgtt gccttcttgc ccccactatg cccctatgaa cagtccgaac gaatacgtcc
     2581 tcgctggtgg cggaaccggc gggcacttgt tccccggctt ggccgtggcc gcagcgctgc
     2641 gagctatcga acccgacgcg gttgtaacct tctttactac tacgcgggcg ctcgatcgcg
     2701 acctgttggc gcgaacgcct tacggccagg tgcagcaaac ggtgcgtccg ctgaccgtcc
     2761 ggccctggcg aattccggcg tttctgtcgg cctggttcaa gaatgtcgcg gccgcttctg
     2821 cttggctcaa ggaccatcaa ccgcgcgccg tcctcggcct gggtggctac gccgccgggc
     2881 cgccggtcgc cgcggcacga cgactcggca tccgcacggc gatcctcaat cccgacgcga
     2941 taccgggacg cgccaatcgc ttcctcgcca gccgcgtcga tttggctgtt ttgcaatggg
     3001 atgtcacgcg cagatacctg tcaaagcgaa cgcactgcca cgttctgggt tgtccgatcc
     3061 gggcagagtt cgcgcgaccg attgacgcgg gcgatgcgcg acggagcctt gggcttgatc
     3121 cgaatcggcc gctgttgctg gttacgggag cgtcgcaagg cgcgcgatcc gtcaatcaga
     3181 cggtctgccg cgtgtggcct gaattccagg ccaaacatcc cgactggcag cttctgcacc
     3241 tgacgggcgc tgcggacgaa gccgaaactc gccgcgctta cggccccaca ctgctcaatg
     3301 gcactggcac aagcgaggcg cgcgggtcgg tccgcgtggt cgctttcatg cacgaaatgt
     3361 ggcttgcgct ggccgccgcc gacctggtcg tgtcgcgggc cggagcttcg acgctggccg
     3421 agattaccgc tctgggaaag ccggccatcc tgctgccata tccttatcac aaggaccggc
     3481 atcaacatgc gaacgccaag gtgctggtcg atgcgggcgc ggctgtgctg atcgatgatc
     3541 agaagaccgc cgaggcgaac catcgcccgc tcgcggacgc actcgagcgg ctggcagacg
     3601 ggcccacgcg gcagcagatg gcgcaagccg ctcgcaccct cgggcggccg gccgcggccc
     3661 gcgacgtggc cgcgtggctg gcgggccggc gcgcggcggt ggaccgcttg cccattgggg
     3721 cccataatcc agcccatggc ggataagaaa cgcggcaaac tggtcgtcat tagtggccct
     3781 tccggcgccg gcaagagcac gatttgcagg gaattgctcc gcgcgctgcc gaacagccgc
     3841 tgttccgtgt ccgccaccac tcggccgccg cgagaaggcg aagttgacgg gcgcgattac
     3901 cacttcatct cacgggcgga gtacgaccgg atggttgcgg cgggcgagct actggaacat
     3961 gccgaatatt gcggccaggg ctacggcaca ccgcgccgcg cggtcgagca ggccttggcg
     4021 cgcggcgaga ttgtcattct cgcgatcgac atacagggcg cggcccagat tgcgcagcag
     4081 gcgcccgagt ccgtccgcat cttcatcctg ccgccaacga tggagacgct gcaggcgcgc
     4141 ctggaaggcc ggcaaacgga atccgaggcg caagtggccc gccggctggc cacggcgagc
     4201 catgaaatcg ctcacgcccg cgccagcggc ctctaccatc atttcgtcac taacgatata
     4261 cttgaggatt cggttcagcg ggtcatcgaa atcatccagc aggagaacga tcgggaatga
     4321 tcaatccttt gcagaacgac gaccttatcc gtaaggccgg cggccggttc aagctcaccg
     4381 ccctgattca aaagcgctgg ctggaactca tgcagggttc ccgcccgctg gtggatccaa
     4441 ccggcaagac caccatggaa atcgtcatcg atgagatcgt gaacgataag atcgccatcg
     4501 attatgaggc ctccggcatt gcccgcccgg gataacgagt cgcaggccgg cggcaacgtg
     4561 cccgcgggcg ctgcaaacgg tcagccgctg gccggctacg aggtcttggt gtgcatcagc
     4621 ggagggatcg ccgcctacaa gaccgcggtt cttgtttcac agcttgtaca gttaggctgc
     4681 ggcgtcacgg tagcgttgac ccgcaacggg cggcgcttcg tcggtgaggt gacgtttcgc
     4741 ggcctgaccg gccggcccgt gtacaccaat gccagcatgt ggcacacggg cgagcagatc
     4801 cacatgcgtc acctggggct gaccgaggcg gccgacctgg tcgtcgtcgc accggccacg
     4861 gcgaacgtca tcggcaagct ggcgagcggc atcgcggatg atctggtgag cacgctgcta
     4921 ttgggggcgg acagccccgt tctgctggca ccggccatga acgaacgcat gtggcaacac
     4981 ccggccgtgc ggcgcaacgt ggcctggctc cgcgaggccg ggttcctgtt cgtcggtccc
     5041 gaagaaggct ggctggcgtg ccgggcaatt gggccggggc gcatgagcga gccggaggtg
     5101 ctgctggcgg ccattcgcga gcagctcctg aaagcgccgg cgaagcgcat gggggcgcgg
     5161 taaggggctg ggggaagcag aaaaattgcc cgcttccgcg atgggacgta tgggcggaga
     5221 agaccggcaa gcgctggggg aagcagaaaa attgcccgct tccgcgatgc atgaagcaag
     5281 cggtccggca agataacctg ccggaccgca aggggattat tacagatcga actgaatcga
     5341 gattaccgcc gacgaagcag cagggcaaca gcgatcagca gaaggctgga cggctcgggc
     5401 gtcaccacga gctgccaatt aacgtcaatg gggttcgagt ccgcatcgag gatgggcgtc
     5461 gtactaaacc cgaatccggc accggggttc cactggtagt cgttgttatt ccccagcgac
     5521 gtttggtaga tgtacgactg accgctcgtt ccgaactcac gaaccgactg aaaaaccatg
     5581 tagtagctgc ccgccggcag cagttggtgt ccgaaatcgg cgccaatcaa gcccgccgag
     5641 aacagcgtgt catagccgcc cgaggccgac attacgatgg acccgccggc ccacggcaac
     5701 ccatcgtaga tgtggaaatt ggcgccgacg ccatcgacgc caccgttagt gaagcccagg
     5761 gcagtaaccg tctcgcagta gtaatcttcg taagtcgtga aatcggaaac cacccagatg
     5821 tcgtaggcgt catacgccgt ttcgaaatcc tgggcggcgc tgccgtactg ctggacttcg
     5881 tcatacggat ttcccagcgt gccgcgcacg gcgggcgaca tggcagccgg gccgacggcg
     5941 cacgggctga tcgtatcagc cagcgcggcg ctcgcaagca aaaacacccc caacaccact
     6001 atacctacct tcatcgaata cctccttctc cagtaactcc ttgtcatata gccgggatga
     6061 aggaacgccc ttcatcggcc acgtactacc tcctcagatc gatctgctcc gacgatgcga
     6121 cctgtggacg cagctctagt gttagaaact gaggcgtagc ctcacatcga ttcggaatca
     6181 cctctttcct cagtcgacga tcacagaacc gttcacgacg acgcacatcc ggaatcaaga
     6241 tgatctaata gtcactatac ccttagcatg ccctgacttc aagacaattt gtggtttgtc
     6301 atcgctttcg cgaacgatgg cccaacgata cggaacgagc actctacgtc cgtattttgg
     6361 ttatagggcc accacatcgg acatagcaga accagcccca gttgccatgg acttatcggt
     6421 gccattggtg ccgtaggtgc cacgcccaag ctataagcgc gggcatgccg cgccaatttg
     6481 cctttccgtc acgccgacta gttgcggcaa gcgcacaatg tgtatgccga cactccgcaa
     6541 ccatgccgtc ggattgagca tagcgtcact ggcggcaccg ccagctctct cgttccgcca
     6601 taatcaatcg gataaccaca ccgactcgtg atcgaacgcc gagccgcgcg tagagcgccc
     6661 gcaggtgcgt cctgacggta ttagccgata ctttcagccg ctgtgaaatg tccctttgcg
     6721 aatactccgc acatagcaac tccgccaccg cacgttgccg cggtgtaagc cgtagcactt
     6781 tcgcaatcac gggccactgt accggcgtga acagcacggg caattcgcaa gcaacccccc
     6841 ctgccctttg aaaccgtcta cgccggcgta ccgagctact cagagtcatg caacctcact
     6901 cctccggcca tgggggtgcc ggcggccata acgctgaaga tgtataccgt tctcaacgac
     6961 ggtggcgtgg ctgctgctgc caccaaccat atcagcgcca tccgcccaca aaagcgcagc
     7021 gattatttga attttttcac ggactggaac gagccggcga ctcggccgcc ggctgagaac
     7081 tgggcgagag gctgcccagg ctagcgcgca tttggcccag caggtcctcg gtatcggacg
     7141 gtgggagacg gcccggctgg cccgattgcc gcaggtcgcg gagacgcgtc aggaagtaat
     7201 ccgcttgact atgttcgcca cgggccgccg cgaattcgcc gcactcctga agggtgcgtt
     7261 caagatcccg ccgagtcatg aggtcctccg gatccagctc ggccaatttt tcgtttattc
     7321 gcagcgcgcc gcggcgtaca accgggccgc ttcatccggc tccgttccgc gaatctgacc
     7381 ggcgacccgc accaaggcgg acgcctcggc actgaggaag cgccggttat gcggatcgcg
     7441 cgacacgata tggcgggccg cctccaaacc ggcgtaggcg gccgcccgcg cggcttcacg
     7501 gctcgcctga atgtcctccg cctgggtgtg gcgatccgcc ttggcgttct ccagcatgca
     7561 gattatctgg ttaatttccc agataccgtg gtacgtaatg aggttatccg gttcgatcgc
     7621 cagaagttgc tcattgatcg tcagccgctg acgacagtgc gcctcggcct cgtccagccg
     7681 gccttcacgc agcggggcgg ccagtcgatc gtgcccccag gcgacgtggt ccaaatgccg
     7741 gcggaccccc gggttttgcc ggaccagctc ttcatccatg gccagtgctt cttcccacag
     7801 acgccgcgcc tcgtttcgct ggtccagcgc gttcatcacg tcaccgattt gcacgagggt
     7861 caaagacacc tcggccccac gctgcgccga ttgcggttgg gatgccagca ggcgccgccg
     7921 cagttccagt acgcgcttcc gcaatgtcaa agcttcgtcg tggcgcttct cgctgagcag
     7981 taactccgcc tgccgcgccc aaacggcggc gcgcgccgtc agaagctgct catcctccgg
     8041 ctctcgtcgc agcaactcgt caacctgcgc gtccagccgc tccagtagcg tacggcgaac
     8101 cgcggccgtt cctgccaccc ggtcgagatc tttggcagtc cgttcgacga ggaatgtggc
     8161 cgcgtcgatc gtctgacgcg acaggtcttc aagcgcgctg gcggattgcc gggcccgcat
     8221 gagcccatag ccggttccga acaatcccac aaacaggctc aacagcgcga tgcccgcggc
     8281 caccaccaac atgcgatgcc gcgccaggtg tctccgcagg aggtacagag tgcttggcgc
     8341 ttgtgccaga atcggctcgc ctgccaggta gcgccgcaag tccgcgccca gcgccgcggc
     8401 cgactcgtag cgctcatcgg ggttcttgcc gagcgctttg aggataatgg tgttcaggtc
     8461 gccgctgaga ctacggtcac gtgccgccag gggcagcggc gaactgtcgt gaacggcgcg
     8521 cgcggcggcg taaaaggacg actcgggaac ttcaaccggc agggcgccac tgagcagttg
     8581 ataacccacc acgcccagcg agtagacgtc gctgcgcgtg tcgactgcgc gcgggtcccc
     8641 atcgatttgc tcgggactca tatagcgcag cgtccccagc acctgcccgc ataccgtaac
     8701 ggccgtggca gacgcgtcgc ggccggatac gcgcgccacg ccgaagtcga tcacccgtgg
     8761 ttgcgcaatg ccatcgagtt gctcaaccag gatgttggcc ggcttgatat cgcggtgaat
     8821 caccccccgc tggtgcgcat gcccgaccgc ctcacagata cgtgccagca gctcaatccg
     8881 ctgccgccgg ctaagcggct gctcattggc aaacgtcagc aatggcgggc cgtctacgta
     8941 ctccatggct atgtaaggtt gggcgccatc gggcgttacg acagtaccgg cctcatgaat
     9001 ggtggcgata cccgggtggc gcagcagcgc caaagtctcc atctccgtcg cgaatctgcg
     9061 gcgcgcttcg cggctcatga cgtccgggcg aatcagcttc aacgccaccg tgcgtggcgg
     9121 agtctgctgc caggcgcgat agacaacgcc catgccgccc acggcgatca agttcagtat
     9181 ttcgaaagcc ccgatacgct ccggaagcgg agcttgatcc gcaggcacac aaaaatccgc
     9241 cacacgcaca gacggcagaa gcggtttttc cagaaccgcg gccggcgtat cacaagccag
     9301 gagcgactcg atctcgcgct gtagctcaag gtcgccgccg caggcctggg ccagaaagtc
     9361 gtcccgctgc ccgggcggca tttcaacagc cgcattgaat agctcgacta tccggcgctg
     9421 gcgcgtcggg ccggcattcg tgtcggacgt catgacagct cgcgcatcag ccaggctcgc
     9481 gcccaacgcc agtcgcactg taccgtgcgg cgcgcgatgc ccagcacatc tgccacttcc
     9541 tgctccgtca ggcctccgaa gtaccgcagc tcgacaatct ttgctgcccg aggtgattcg
     9601 tcccgcagcc ggttcaacgc ctcatccagt tggacgacgt cgatcggcgg ttcagccgcg
     9661 gcgacggccg catcatccaa ccgcatagtc acgcatgccc cgccgcgctt gagacggcgc
     9721 cggcgccgcg catgatccgt gagaatacgg cggatcgccg cggcggccgc cgcgcagaac
     9781 gccgcccggt cgttcacggg ccacacgttt ctgcgggcca gtcgcagata cgcttcgttc
     9841 accagcgccg tcgtttggag cgtgtggttg gcgcgctcgc gccgcaagca ggcggccgcc
     9901 agggaccgta actgtgagta gaccgcctcg gccaaatcgg ccgtcaatgc cacgccggaa
     9961 ccggcggcct cgcctaggtc ggccgtggat gcctcactcg aatcggacgt caatgcgtcg
    10021 ccgttgtcag atttctggct cgccaacgca gtttccatgc tatctcggaa cggatggaca
    10081 ggatcggttt ctcgattatg cccgaccgcc cccgccctgg tcaatagaaa atgcgggcgt
    10141 tgcgggtgtt aagagcccgt agtgcagggt gctatgccca tgctaaaggc gcgcgcatgc
    10201 gagcacaggc gccataacaa tagggaacca cacctgactt aatgggcagt cgcagcaaca
    10261 gggtgctgcg cggatgcgga ggctacacgc tttcgacttg ccagtattcg atttcgatcg
    10321 gcgtagcgcg gccgaaaatc gtgacgatca cgcggaccgt gcccttttgc gtgttgatct
    10381 cgtccaccac gccttcgaag ttctcgaacg ggccctcgtt gaccttgacg cgatcgccct
    10441 tcttgaaggc caggttcgcc agcgctgccc cttcttccgg ccgaatggcg gcggcgagca
    10501 tcttttccac atcgtgcatg ggcatgggcg acggcttgcc gcccgaaccg atgaagtcgc
    10561 cgacgcccgt ggtttccttg atgacgaacc aaacccgctc ggggatgcgg ccgtcctcgt
    10621 cggtggccat ttcgacgaac acgtagccgg ggtagagctt gcgatggacg acgcgggccg
    10681 agccgccgcg gatgcgcttc tcgcgttgcg tgggaaccag cacgcgcccg acgcgttcct
    10741 caagctgctc gatcttgatt ttgcgctcca gggcctcgcg gacgcggtcc tccttgttgg
    10801 ccgccacccg cagcacgtac cagttcatcc cgggccggac caactccggc tcggccttca
    10861 cggcggcagc ggtagcaggc gcctcagcag gggcagcttc gcgggcctca gccggcggtt
    10921 cttccggggt ctctttggcc ggcatctccg tcattgcgcc attccaaacc agctcttcca
    10981 caaaggtatc ttcagcacgc ccatgccgct gaaggccagc atgaagagtg tatccacgat
    11041 gaagagcaaa aacccgagcg ccaggacggt aaaaatcacg accttggtcg cgcccagcac
    11101 ttccttgcgc gacgaccagt tgaccttctt catttcccct tcggtcgcga tcatgaaatc
    11161 gaccgtgccg tgcttctgga ataccagcca gaagatgagc cagcccagcg ccgccagcac
    11221 cgccaccgga atcagcgtgc gcagcaccag attgtcgccc aaggggagcc gcaaccattc
    11281 ataaaagaat cgcacaccgg ccacggcgat aacaccggcg ccgatggccg agccccagcg
    11341 gacatgcttg ccttgcccgg gcttgtaaat tttcagcggg ctgaccgcgg ggcgcgagat
    11401 ctccgacgga cgcgccggtt cgcggggctg ttcaaccgaa agactatcgt ctgccattaa
    11461 gcgctccacc ggagcctccg ccgctcgcag gtcggggtgt ttcaccggat tcagctcagt
    11521 acgaaccaac tggcggccaa cagcggccga cccagggatc aggagcggag ggactcgaac
    11581 ccccaacctg ctgatttgga atcagctgct ctgccaaatt gagctacgct cctaaaagct
    11641 ctcagctatc agctctcagc tatcggccag agagcgcctg gctgaaggct gacagccgac
    11701 ggctgatagc tcaccttact ttctctttat cttatgcgtc gtgcgtttac gctcgcgcgg
    11761 acaatacttg gccagctcca gcttcggaac gccgccctga acgtttacct cagtccgata
    11821 gttcaggtcg gagcactcct tgcactgcag ccagacccat tctcgctttt gcgccttcgc
    11881 catgaaaagc tctcagcttt cagctttcag ccagacctca gctcccagcc catccggctg
    11941 ggagctgatc gctgacaggc ttattccaat atcttcgtca ccacgcccga gccaaccgta
    12001 cggccgccct cgcgaattgc gaaccgcagc ccctcttcca tggcgatcgg gttgtgaatc
    12061 tcgacccgca tctgaatgtt gtcgcccggc atgcacatct cggccttgcc gccgtcacgg
    12121 gcctgcagct cgatgatcga gccggtcacg tccgtcgtgc ggaagtagaa ctgcgggcgg
    12181 taacccggga agaacggcgt gtggcggccg ccttcctctt tggtcagcac gtacacctcg
    12241 cccatgaact tcgtgtgcgg ggtgatgctc ttgggcttgg ccagaacctg gccgcgctcc
    12301 agttccttct tctcaacgcc gcggagcagc agaccgacgt tgtcgccggc gatgcccgat
    12361 tccagcgtct tattgaacat ctcgacgccg gtcacgaccg tctggcggcc ggccggtgcg
    12421 tgcaggccga tgatctcgac cgtgtcgccc acgcggaccg tgccgcgctc gatacgcccg
    12481 gtgccgaccg tgccgcggcc cttaatgctg aacacgtctt cgacgggcat caggaagggc
    12541 ttgtcctgct cgcggacagg ctcggggatg ttggcgtcga gggcgtccat caactgctga
    12601 atcggaccgt agccggcctc gttcttgtcg aaggcggcct tcagagcttc ggtggccgag
    12661 ccgcggatga ccggaatggt gtcgcccggg aactcgtact tgttgagcag ctcgcgcact
    12721 tccatctcga ccaactcgag cagctcgggg tcatcaacca ggtcaacctt gttcaggaag
    12781 acgatcagcg ccggcacgtt cacctggcgg gccagcagca cgtgctcgcg cgtttgcggc
    12841 atcgggccgt cagcagcgct gacaaccaga atcgcgccgt ccatctgggc ggcgccggta
    12901 atcatgttct tgacgtagtc ggcgtgcccg gggcagtcaa tgtgtgcgta gtgacgcttc
    12961 tctgtctcat actccacgtg agccgtagcg atggtgagaa tcttggtcgg gtcgcgccgg
    13021 ccgtcctttt cggacgcctt ggcgatctcg tcgtacgact tgtagacgcc caggccgcgg
    13081 gctgcctgga ccgccgtcat ggcggccgtc agggtcgtct tgccatggtc gacgtgacca
    13141 atggttccca cgttcacgtg cggtttagtt cgctcaaata cgcctttggc catttatgtt
    13201 ctccactcat cgcccgggca acgttagccc gggagcagcc taccgcggcc gccaattctg
    13261 aatacctttc aggttcgcca cctgttccag tggttcggcc cgcgaaaggc tcgctgaaag
    13321 ctgctggtgg ggattgaacc cacgacctcc tccttaccaa ggaggcgctc taccactgag
    13381 ctacagcagc gtccccagcg cagcccgtcg gctggccgcc ggccgtacat acaccgagac
    13441 acgcccggtc aattcaaccg gaacggtgta ttgtaagtcc gtcgtaaatc ttgtcaaagg
    13501 ggaaaacggc ccc
//
LOCUS       DAOSYS010000080        13444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3870_length_13444_cov_4.925909, whole genome shotgun sequence.
ACCESSION   DAOSYS010000080 DAOSYS010000000
VERSION     DAOSYS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3870_length_13444_cov_4.925909"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLU99_06715"
     CDS             complement(<1..332)
                     /locus_tag="PLU99_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOO16784.1"
                     /translation="MPQDILCETLEAVGVVRINRPEALNALNPAFLEQIVAALERYDA
                     DAGIRCMLLAGSERAFSSGYDLSELSATAPIDVYRNNLFARWERLRAIRKPIVAAVSG
                     YAVSAGCEL"
     gene            complement(440..1648)
                     /locus_tag="PLU99_06720"
     CDS             complement(440..1648)
                     /locus_tag="PLU99_06720"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOO16785.1"
                     /translation="MSSFWEPLRHYQLYGWTSFVLALLLMPLAMRVARRFRVMDYPDT
                     FLKPHARPIPYLGGTAICLAWATALVLALSRGVEVRLLLPMLLGGLAISTLGLIDDVR
                     GVSPKARLLVGAVVVLAVLISAGAGFRLVEAITHPIAEFAAYFFGVHIPPVPEVIAIP
                     VSLLISVFIVLGACNSTNLIDGVDGLCGGVTGIISFGFFLLASWLASWGYSPPGDPVR
                     LVLAISMFGAALGFLPWNFNPARIFMGDAGSMLLGFNCGMLILLFAEKGIARWMIGAL
                     MIFALPVFDTALAMFRRWRSGRSIFEGDRSHFYDQLIQRGWSVRQTVLICYGLTVLFG
                     AIGLLVTWIGPDGVPVIRTRYAVPLYGLVCVAVALGASLAGLTHPEDKRPAGVATQSD
                     SSSATAESRS"
     gene            2232..2369
                     /locus_tag="PLU99_06725"
     CDS             2232..2369
                     /locus_tag="PLU99_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16786.1"
                     /translation="MAAGTYLGNNAEQKLMAVVDGLLSDMTDFRRKSAVQLAPLSAIA
                     V"
     gene            2609..2932
                     /locus_tag="PLU99_06730"
     CDS             2609..2932
                     /locus_tag="PLU99_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO16787.1"
                     /translation="MGKKLYVGNLSYSVSSSDLEELLSRHGTVQSAEVISDRVTGQSK
                     GFGFVEMASEEEAQAAITALNGQVHDGRTLTVNEARPREPRAGGGGRGGYGGGRGGRG
                     GRDRY"
     gene            3008..3577
                     /locus_tag="PLU99_06735"
     CDS             3008..3577
                     /locus_tag="PLU99_06735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOO16788.1"
                     /translation="MSEVMPFGWTGKKVRLVPLEYEKHFANALRWLNDPEVTRWLLIG
                     DYPITRVAEEAYFQRAAESSPSAQDPAEVLFAIETLSQEEHIGFCGIHQISFRHGVAI
                     TGTVIGRRSLWNRGFGSDAIAVRTRYAFEVLGLRLLLAEVFAENGGSLKALARNGYRE
                     VGRIPQRWWKRGALRDTVQLALYRQDWKP"
     gene            complement(3829..5115)
                     /gene="tyrS"
                     /locus_tag="PLU99_06740"
     CDS             complement(3829..5115)
                     /gene="tyrS"
                     /locus_tag="PLU99_06740"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016362872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOO16789.1"
                     /translation="MKTDFLADLQQRDLVHQLSAPEVTALLSEQRVTGYIGFDPTADS
                     LHVGSLLPLITLMRFQRAGHRPIGLIGGGTGLIGDPSGRTSERSLLDRERLAQNLAGI
                     RRQVERFLDLSGDALLVDNAEWLESLNLIDFLRDIGKHFSVNQMIARDSVRMRLETRE
                     QGLSFTEFTYMLLQAYDFLALFDRYGCRLQMGGSDQWGNILSGCDLIRRLRGAEAYGV
                     TMPLVTRADGRKFGKSEEGNVWLDGRRTSPYQFYQFWINVDDADVVRYLKYFTFLPLP
                     EIAEIEREVAAAPEKRTAQRVLAEEVTRLVHGPEALARAVRATSVLFRKDADYRELTA
                     QELEEAFHGAPTATLDRAVLGTPEAGLVALLAQVGLYSSRSMARKDLPSGAVTVNNRP
                     VRELDRVLTESDLLPGGFIILRKGKKHHHVLRVRQS"
     gene            complement(5254..5649)
                     /locus_tag="PLU99_06745"
     CDS             complement(5254..5649)
                     /locus_tag="PLU99_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16790.1"
                     /translation="MSVMQNLTQAREELIAQRAALDEQLAALDTALSAMGRRPMAARP
                     APGGPALRAGGRRPQGVAAGRRPGSLKEHIARVLSATGGPMAVKDITEGVRRGGYETR
                     NKTLAKSVGIALTQMPEVDKVGRGLFRLK"
     gene            6209..7843
                     /locus_tag="PLU99_06750"
     CDS             6209..7843
                     /locus_tag="PLU99_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16791.1"
                     /translation="MRRPARRLMCLYLPFLSTDQVRRLEDGGVLAGPLVLTQAVGLAQ
                     KIACLDAQALQAGLRPGMSLAQARALLPEVTTRPHDSQRDQIVLQKLADWAVRFSPLV
                     DPCPPQSLLIDITGCEPLFGGQERLATLVLAGLAEAGFFARAAVADTVGAAFALATTA
                     RQPVSIIPPGQTAQYLAPLPPTALRLEPQAAARLEALGLSTIGDLLKLPRQALPARFG
                     QNLNLRLQQALGETFEGIPVRLPRTVPYARLEFEQPIRDLLAVQAAAADAFKDVFNQL
                     QRRDEALRRLDVLLLSETPPQRLAIGLARPSRRRQHVWQLLLQRLEKCDLAQGITGLV
                     LMAGDVSRSQVIQGELFQPDQQPQQDEQLGMLLDRLANRLGRGSVVRATRVDDYQPEL
                     AVGYSPAGCESEQPRANTPAKDALEADSSSTARSAGERGRPREAKAGKRGKARREAKP
                     ASRSTPLRPTQLFSRPTPIRAISKVPDGPPTWFCWRNREYIVHAAWGPERIETAWWRG
                     PDIRRDYFRLETQSGEQFWVYHNFDDQRWYLHGIFA"
     gene            8062..8913
                     /locus_tag="PLU99_06755"
     CDS             8062..8913
                     /locus_tag="PLU99_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16792.1"
                     /translation="MNAHTHRLAAVALIAAAMLSAKAAEPPVTYLTPDTFKPAIGQTL
                     TLRVNSGTPQRAEVAPWPEDQLRYFFVRGGGMQHNRPTLPATERDRNAAEFPISQHGV
                     SVIALEPKPAVVQMSGEELKSFLLRNVAGAERDARVAALSEKATVRVRRVESATALVR
                     APEAAGEQAGPSSIAMSKLGLRVELRSLIDPTVSPAGSDWAVRAYIEGSNKSNVQVQA
                     TSLADGKTQTALTDRSGAARFHFGVGGSWRVEFHQAQPLENDSQADWVLYSGTLTFEV
                     PTKGAGK"
     gene            8910..11213
                     /locus_tag="PLU99_06760"
     CDS             8910..11213
                     /locus_tag="PLU99_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16793.1"
                     /translation="MTRNEKSPARTLLSRTAALGLLVAVCSAASALEWEELGPAPILT
                     GRYTGRVSAIVCSKTNPDLYYVAGADGGVWRTYDGGATWTPLTDFMPTTAIGALAMDP
                     VDEQIIYAGTGEANYAHHSRFGLGLYKSTDGGDNWETLAAEVFSGRCFSRLLIDPVNP
                     QRLYAAITPAGGFPALCAAKGHPGATGPVGVFRSDDAGVTWTQLLNGLPNLAATDLAM
                     DPVNPAILYAAIGHIFGSPENGIYKSTDGGDSWTKLGGGLPTEKVGRISVSVAPSLPT
                     RLYALICHSSDEAGGGASTMGAYRSDTAGATWETYVDFPTIQGTYGWYLSIVGIDPAN
                     PDIVFMGGVTLVRSGDEGIWHTVTPPHVDMHAIVWDAAGRVVVGDDGGVHRSEDGGWT
                     WSTINEGLGLIQGYAGFSRHQQEYYMFIGTQDNGTLRRNANGTWTNILGGDGGWTQTD
                     PVSTSRVFAEFQGTGNLYRSTSLGSSFTFVGTGISSGDRNCFMPPYLIDPNDSAKMLY
                     ATHRIYRSTNYGSNWTAISSDLTGGGSAAVRSLAMAPSDPYTLYASTNDGRVLVSTNG
                     GYTWTIIRENVPGWSRIMRQICVHPTDPQTAYVAVSAFGEQQILRTTDQGQTWTALDG
                     NFPDIPANVIAVDIRTPRPTIFVGAEDGLYRSVNDGVSWHRYGAGLPRCPVIDISFEP
                     DRSRLIVSTQGRGVWRIPIGVPADMNGDTMVNAFDIDAFVLALTDPAAFAVQYPTVDV
                     IANGDINGDGLLNAFDIDGFVGVLTGA"
     gene            11342..12202
                     /locus_tag="PLU99_06765"
     CDS             11342..12202
                     /locus_tag="PLU99_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO16794.1"
                     /translation="MVKWAVRVGLPLFILTYVGCVGLDGFFYYPNKKTYDRPEDYNLR
                     YEEVRFQTSDGLTLAGWFLPAVRHKPFRGTIVHFHGNAANISGHLLLVYWLPLQGYNL
                     LMFDYRGYGQSEGKPTRAGTIADGNAAIDYALSRADVRGGPLFVYGQSLGAAVAVVVA
                     AERPEIAGVVAESPFSGYRRIAARHLQKRILIPPLARGLAALCVSGGYDPVDAVPRLA
                     PRPLLVIVAGNDNICFPDLGRELFDAACEPKEIWEAPGADHLAIMEGNANALMDRITG
                     FFDRAAEGRR"
     gene            complement(12218..13057)
                     /locus_tag="PLU99_06770"
     CDS             complement(12218..13057)
                     /locus_tag="PLU99_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16795.1"
                     /translation="MTRCICLLLFCALCAALTGCPPQPGNQNANDNVSPSLTAAQQQA
                     IQTGMTQIEALFEIFSTLNAFADPRLALDTLGPIESFGECPVVSYVTDAARVNALIAV
                     DFGAGCSSLITANRVVSGLTTSRVNLTTRVGTIDFTTLVFDGGTIQGQLDPVTISSDP
                     AGTNLAGTMSVNTAAVGALVGNFDVTLMQRGQMRINSAIFTVENGPPTQITLTGAIAD
                     PSLTLNLIPFQGSAQFDIPSAAGAATEQALLTFTTETAQNGIIQVQAPDVPATDFQVP
                     GLL"
BASE COUNT         2616 a         4192 c         4212 g         2424 t
ORIGIN      
        1 agctcgcacc cggcgctgac ggcatagccg ctaacggcgg cgacgatggg cttgcggatg
       61 gcgcgcaggc gctcccagcg ggcgaaaaga ttattgcggt aaacgtcgat gggggcggtc
      121 gcggacagct ccgacaggtc gtagccggag gagaaggccc gctcgctacc ggccagcaac
      181 atgcagcgta tgccggcatc ggcatcgtag cgctcgaggg cggctacgat ttgttcaagg
      241 aacgccggat tgagtgcgtt cagcgcttcc gggcggttga tgcgaaccac gcctacggct
      301 tcgagggtct cgcaaaggat atcctggggc atgcgttggt ccctgactgt ctgagttctc
      361 ggattggcct cgctctcgac cgggaacctg tgctccgacg ctaaagccga ccggtcgaga
      421 agcggtcttg caagtacagc tagctgcggc tctcggcggt ggcggaactg ctgtccgact
      481 gcgtcgcaac gcccgcaggc cgcttatcct ccggatgcgt cagacccgcc aggctggcgc
      541 ccaatgcaac cgccacgcag accagaccat acagcggcac cgcataccgg gtccgaatga
      601 cgggcacgcc gtccggacca atccaggtta ccagcaggcc gatcgctccg aagagcacgg
      661 taagtccgta gcagatgagc accgtctgcc gcacgctcca gccgcgctgt atcaattggt
      721 cgtagaagtg gctgcgatcg ccttcaaaaa tcgaccgccc gctgcgccag cgccggaaca
      781 tggccagcgc cgtatcgaag accggtaacg caaagatcat cagcgcgccg atcatccagc
      841 gtgcgattcc cttctccgcg aaaagcaaga tcagcatccc acagttgaac cccagcagca
      901 tgctgccggc gtcgcccatg aagatccgcg cgggattgaa attccacggc aggaatccga
      961 gcgcggcccc gaacatggag atcgccagca ccaggcgaac cggatcgccg ggtggactgt
     1021 agccccatga ggccagccac gaagccagca ggaaaaaccc aaacgagatg atgccggtca
     1081 ctccgccgca caggccatca actccgtcga tcaggttagt tgaattgcag gcgccgagca
     1141 caataaagac actgatgagc agcgaaacgg gaatcgcaat tacttctgga accgggggaa
     1201 tgtgcacacc gaaaaaatac gccgcaaatt cggcgatggg atgagtaatc gcctccacca
     1261 gccggaaacc cgccccggcg ctgatcagaa ccgccaaaac cacaaccgcg ccgacaagca
     1321 ggcgtgcttt gggcgaaaca ccgcgcacgt cgtctatcag gccaagcgtc gaaatggcca
     1381 agccgcccag caacatcggc agcagcaagc gcacctctac gccgcgcgag agcgccagta
     1441 ccagggcggt cgcccaagcc aaacaaatcg ctgtcccgcc gaggtacggg atggggcgcg
     1501 catgaggctt cagaaacgtg tcgggatagt ccatgaccct gaaacggcgc gctacgcgca
     1561 tcgccagcgg catcaacagc agcgccagca caaaggaggt ccagccgtat agttggtaat
     1621 gcctcagcgg ctcccagaac gagctcatgg cggctcaata taaccgaacc tgggcctaag
     1681 cgaagcgggg cggccaaatc gcaaataacg gaccggaggg agcgaaaaag caccgacggc
     1741 tggcgtccag gcgattgaac cggcgcagaa aatgcgccac gccgagaatt tttgcgccaa
     1801 aagaaatttg tgttcaagta ggtcgcggcg tctggaattt tgcccatttt aacacaagac
     1861 gagcgtcgca gggccgatgc gttaagaatc acgatttatg gggcctcgcc atgctagcct
     1921 gctcgctaac gtccctccca acgagcgcaa gaacattcaa aggaccactc gataacagaa
     1981 gtagcccgag cgcgagccgg gcggttgggt gcgggtcggg gtgacccgcg agcgaggtgt
     2041 tcaggaaaag atagggtgcc agcatgagcg gtcttacacg ccgtccgcgc cggacgttaa
     2101 tcggcgagcg ccgcctccgc ggcaaagtac tttggcagca aagatttagc ctcggccccg
     2161 acgcgccggt tccgcggcca cgccatcggc ccggtgccac gcccgagcgt atcgcccaga
     2221 tcaggcgtga attggcggcc ggcacttacc tcggcaacaa cgccgagcaa aagctgatgg
     2281 ccgttgtcga cggtctctta agcgacatga cggattttcg ccgaaagagc gcggtgcagc
     2341 tcgcgcccct ctcggcaatc gccgtttgac aaaaacagtc ctgggacccc accggtctcg
     2401 cacgccggtt tacaagaaac gaaccggcgt tatactgaac gtagaaggtg cgtcgaacga
     2461 ggcgcgtacg tgacctgttt gttcaagtcc ggatcgcact gcctttatcg cctccttacg
     2521 ctccggccgc gcgtaggccg ctttcaacac ggccctgaaa gtgaaaaatg tagggtcaaa
     2581 tgtttctgta tcgaagagga gcatgacggt gggcaagaag ttgtatgtcg ggaacctcag
     2641 ctatagcgtt agcagctcag atcttgagga actgctatcc cggcacggga cggttcagag
     2701 cgcggaagtg attagcgatc gtgtgaccgg ccagagcaaa gggtttgggt tcgtcgagat
     2761 ggccagtgaa gaagaggccc aggctgcgat taccgcgctg aacggtcaag tgcacgatgg
     2821 acgcaccctg accgtcaacg aggcgcgccc gcgcgagccg cgcgccggcg gcggtggacg
     2881 tggcggctat ggcggcggcc gcggcggccg cggcggaagg gaccgctact aaaattcgaa
     2941 acgaccgcag tcgagaaggc caccgcaagg tggccttctt gctgcgcacg cgcgacagga
     3001 atagaacatg agcgaggtta tgcccttcgg ctggacaggg aaaaaagtgc ggctcgtgcc
     3061 gctggagtac gagaagcact ttgccaacgc cttgcgctgg ctcaatgatc cagaggtaac
     3121 gcgctggcta ttgattggtg attacccaat cacgcgcgtc gccgaagagg cttatttcca
     3181 gcgtgcggcc gaatccagtc cttctgccca agaccccgca gaagtgctgt tcgcgatcga
     3241 gactctgtcg caggaagaac acatcggctt ttgcggcatt caccagatca gcttccgtca
     3301 cggcgtagcc atcaccggca ccgtgatcgg gcggcgatca ctgtggaacc ggggctttgg
     3361 ctcggacgcc atcgcggtgc gcacccgcta cgccttcgag gtcctcggcc tgaggctgct
     3421 gctggctgaa gtattcgccg agaacggcgg ctcattaaaa gccctggccc gcaacggcta
     3481 tcgcgaagtt ggccgcattc cacagcgctg gtggaagcgc ggcgccctgc gcgacaccgt
     3541 gcagcttgcc ttgtatcgcc aggattggaa gccctgacgg agatcccact caagaacaca
     3601 actggcccca aacgcgggtt accacgtttt caagacactg caccccggct cggcccagcc
     3661 tcctacacag tttcaccaca catttttctg tcgcgaaggc aggtcaagcg acgtaggaga
     3721 catggaatga gcaaagtagt agatatgagt atggtgtcat gatgtcccgg gaattcaaga
     3781 ctcgccgcag cgcgcgagct ccgctacggc ccgctccgcg gtttcccctc aactctgccg
     3841 cacgcgtaat acgtggtggt gcttcttgcc cttgcgcaag atgataaagc cgccgggcag
     3901 caaatcgctt tcagtcagaa cgcggtcgag ctctcgaacc ggcctgttgt tcacggtaac
     3961 cgctccgctg ggcaggtcct tgcgggccat gctgcgcgaa gagtacagcc caacctgagc
     4021 cagcagcgcc accaatcccg cctccggcgt gccgagcacc gcccgatcga gggtggccgt
     4081 cggcgcgccg tgaaacgcct cctcgagttc ctgcgcagtc agttcgcggt agtcggcgtc
     4141 cttgcgaaag agaacgctcg tggcccgtac ggcccgcgcc agcgcttccg gaccgtgcac
     4201 caaacgcgtt acttcctcgg cgagcacgcg ctgcgccgtg cgtttctccg gagcggccgc
     4261 cacttcccgc tcaatttccg cgatctcggg cagcggcaga aacgtgaaat acttcaaata
     4321 gcgaacgacg tcggcgtcgt ccacgttgat ccaaaactgg taaaactggt aggggctggt
     4381 gcgccgccca tccagccata cgttgccttc ttctgatttt ccgaatttcc gcccgtcggc
     4441 ccgggtcacc agcggcatcg tcacgccata cgcctcggcc ccgcgcagcc gccggatcaa
     4501 atcgcagccc gagaggatgt tgccccactg gtcgctgccg cccatctgta agcggcagcc
     4561 gtagcggtca aacagtgcca ggaaatcgta agcctggagc agcatataag tgaattcggt
     4621 gaaagagaga ccttgttcac gcgtctccag tcgcatgcgc actgaatcgc gcgcgatcat
     4681 ctgattgacc gaaaagtgct tcccgatgtc gcgcagaaaa tcgatcagat tcaaactctc
     4741 cagccattcg gcgttgtcga caagcagggc gtcgccggag aggtcgagaa agcgctccac
     4801 ctggcggcga atgccggcca ggttctgagc caggcgctcg cggtccagga ggctgcgctc
     4861 cgaagtgcgc ccgctgggat cgccgatcag cccggttccg ccgccgatca aaccgatcgg
     4921 acggtgaccc gcgcgctgaa agcgcatcaa ggtgatgagc gggagcaagc tgccgacgtg
     4981 caagctgtcg gcggtgggat caaatccaat gtagcccgtg acgcgctgct cgctcagcaa
     5041 agccgtcact tccggcgcgg atagctggtg caccaggtcg cgctgctgca agtctgccaa
     5101 gaaatcggtt ttcatggggg agattataag gaccgggcgg ccggccgcga cgcccccccg
     5161 cggcggcaaa acaaagatgc cgtcccggac tacctgccgg agcggcatcc cgaggatgag
     5221 atcagaacaa gcgagagtat ccgtggcgcg cggctatttc agacggaaca ggccacgccc
     5281 gaccttgtcg acctccggca tttgcgtcag ggcgatgcct acgcttttag ccaaggtctt
     5341 gttgcgggtt tcgtagccgc cgcggcggac gccttcggtg atatccttga ctgccatagg
     5401 accaccggtg gcagagagca cgcgcgcgat gtgttccttc agggaaccag gccgccgccc
     5461 tgcggcaacg ccctgcgggc gccggccgcc agcccgcagc gccggaccgc cgggggccgg
     5521 gcgggctgcc attggtcgcc ggcccatggc cgacagggct gtgtccagtg ccgcaagctg
     5581 ttcgtccagc gcagcgcgct gcgcgatcag ttcttcacga gcctgcgtaa ggttttgcat
     5641 cacactcatc ggcgccagcc caccctgtcg ggcgggacga cccgcggcca tccctcttcg
     5701 aaccgctcta ctgcgtgtgg ctttagccat ggcgcatctc cattacatgt tgtacgtcta
     5761 gtatagttgc caaacgaccg cttttcccag tccagcaact atcagatact aaggtaaact
     5821 gctgctgacc gtcaagggct gccccaccat aatactagtc gcgctgaagc gcctttcatt
     5881 ggtgactcta tacccgggtg gcacactgta cctttgggcg cgtgggcgcg gcttcaggac
     5941 cacgtgacgg gttgccgcct cgatttacct aagacgaatc cagagcttac agggcggtta
     6001 tgacgcggcc acgttagacg ccacaatgat gccacgaggg gtcgcgcctg gtcgggttgg
     6061 tcgcattttc agcttcactg cactgttgca gagtcgccgg tggtaactgc accagccgcc
     6121 attctgcgat gcattctgct tgatgtcttg gcaggcatac tatatgttag cagcaagtta
     6181 ggatgcgttt ggcattcaga ggaatcgcat gcgtcgcccg gctcgcaggc tcatgtgcct
     6241 atatctgccg ttcttgtcca ccgatcaggt tcggcggctg gaggacggag gtgtgctcgc
     6301 cggaccgctc gtccttacgc aagccgtggg cttggcccaa aagatagcct gcctggacgc
     6361 ccaagcgctg caggcgggtc tgcgtccggg gatgtcgctg gcccaggccc gcgcgctcct
     6421 ccctgaggtg accacacgcc cgcacgactc acaacgtgac caaattgttc tccagaagct
     6481 cgccgattgg gcggtgcgct tcagccccct ggtcgatcct tgcccgccgc aatcgctgtt
     6541 gatcgacatt accggctgcg aacctctgtt cggcgggcag gagcgactcg ccacactggt
     6601 tctggccggg ctggctgagg ccggcttctt tgcacgcgct gcggtcgccg atacggtcgg
     6661 cgcagccttc gcactggcga ctaccgcacg ccaaccggta tccattatcc cacctgggca
     6721 gaccgcccag tacctggcgc cgctgccgcc gacagcactg cggctggaac cccaggccgc
     6781 cgcccggctg gaggcgctgg gactttcaac tatcggcgat ctgctgaagc tgccacgcca
     6841 ggcgctgccc gcgcgttttg gccagaactt gaatctgcgc ctgcaacaag ctctcggcga
     6901 gacttttgaa ggcattcccg tccgcctgcc gcgcactgtc ccatatgcgc ggctggaatt
     6961 cgagcagccg atccgagatc tgctggcggt gcaggccgcc gccgcagatg cgtttaagga
     7021 cgtcttcaat cagttgcagc gacgggacga ggctctccgg cggctggatg tgctactctt
     7081 gtcggaaact ccgccgcagc gcctggcgat cggcctggcc cggccctctc gccgccgcca
     7141 acacgtctgg cagctacttc tgcaacgctt ggaaaaatgc gacctcgctc agggcatcac
     7201 cgggctggtg ctgatggccg gcgatgtttc ccgcagccag gtcatacagg gggagctctt
     7261 tcagcctgac cagcagccgc aacaggacga gcagctcggc atgctcttgg accggctggc
     7321 caaccgtctt ggccgaggat cggtcgtgcg agccacccgc gtggatgact atcaacctga
     7381 gctggcagtg ggatactctc ccgcaggatg tgaatcagag cagccccgcg ccaatacgcc
     7441 cgcaaaagac gcgttggagg cggacagctc gtccacggcg cggtcggccg gcgagcgcgg
     7501 ccggccccgc gaggccaagg ccgggaagcg cggcaaagcg cggcgcgagg cgaagcctgc
     7561 cagtcgcagc acgccgttac gtccgacgca attattttcg cggccgacgc ccatacgtgc
     7621 gatctcaaaa gtacccgatg ggccgcccac gtggttctgc tggcgtaatc gcgagtacat
     7681 tgtccatgcc gcctggggcc cggagcgcat tgagacggcc tggtggcgcg ggccggacat
     7741 ccgccgcgac tatttccgcc ttgagaccca aagcggcgag caattctggg tataccacaa
     7801 cttcgacgac cagcgctggt acctgcacgg catattcgcg tagccgcggt ggttgggtgc
     7861 catgctcaaa gctgtaagcg cgggcacacc ctacgccggt agcgccgccg gttccagccc
     7921 gacagcccga acgcaacgaa gccaactggt attcaacggg gcgacacgcc ccggctctgc
     7981 tcgccgtgcc ggccgttttt cgtttggatc ccgcgcccct atccgataac atggttgcca
     8041 acttcatgaa ggaggacagc cgtgaatgcg cacacgcacc ggctggctgc cgtcgccttg
     8101 atcgcagccg ccatgctttc ggcgaaagcc gccgaacccc ccgtcactta cctcacccca
     8161 gacacgttta agcccgcgat cggtcaaacg ctgaccctgc gcgtcaacag cggcactccg
     8221 caacgggctg aggttgcgcc ttggcctgag gatcagctca gatatttctt cgtgcgcggc
     8281 ggcggaatgc agcacaaccg gcccaccctg ccggctacag aacgcgaccg caacgcggca
     8341 gaatttccga tttcgcaaca cggcgtatca gtcatcgccc tagaaccaaa gcctgccgtc
     8401 gtgcaaatga gcggcgagga actgaaaagc tttctgttac gcaacgtggc cggcgccgag
     8461 cgcgatgctc gagtggccgc gctgagcgaa aaggcaacgg ttcgcgtacg gcgagtcgaa
     8521 tccgccaccg ccctggtgcg ggcgccggag gccgcgggcg agcaggcagg cccgtcgtcg
     8581 attgccatga gcaagcttgg cctgcgggtc gaattgcgca gtctgatcga tccgaccgtg
     8641 tcgccggcgg gcagcgactg ggcggtgcgt gcctacatcg aaggctcgaa caagtccaac
     8701 gtgcaggtgc aggccacgag cctggctgac ggcaagacgc aaacggcatt gacggatcgg
     8761 agcggcgcgg cgcgctttca tttcggagtt ggcggaagct ggcgcgttga gttccaccag
     8821 gcgcagccgc tggagaacga ttcgcaagcc gattgggtgt tgtatagcgg cacactgacg
     8881 tttgaggtcc cgaccaaagg agctgggaaa tgacgcgtaa cgaaaagagt ccggccagaa
     8941 cgctgctgag ccgcacagcc gctctgggcc tgctggtagc cgtgtgcagc gccgcaagcg
     9001 cgctggagtg ggaagaactg ggcccggccc ccatcctgac gggtagatac acgggccgcg
     9061 tttcggccat cgtgtgcagc aaaactaatc ctgatctgta ttacgtggcc ggggccgacg
     9121 gcggggtgtg gcgcacttac gacggcggcg cgacttggac gccgctgacc gatttcatgc
     9181 caacgacggc catcggtgcg ttggccatgg acccggttga tgagcagatc atttatgccg
     9241 gcacgggtga agcaaattac gcccatcaca gccgcttcgg actagggctg tacaaatcaa
     9301 cggacggcgg cgacaactgg gaaacgctgg cggccgaggt cttctccggg cgttgctttt
     9361 cgcgccttct gatcgacccc gtcaacccgc agcgacttta tgccgcgatt actccggccg
     9421 gcggctttcc ggcgctgtgc gccgctaagg gacacccggg cgcgaccgga ccggtaggcg
     9481 tgtttcgctc ggatgacgcc ggcgtgacct ggacccaatt gctcaatgga cttccaaact
     9541 tggccgccac tgacctggca atggatccgg taaacccggc catcttatat gccgctattg
     9601 gccacatatt cggcagcccc gagaacggga tttacaagtc cacggacggc ggcgatagct
     9661 ggaccaagct tggcggtgga ctgccgacgg aaaaggtagg tcgcatcagt gtgagcgtgg
     9721 cgccgagcct gcccacgcgt ctgtacgcct tgatttgcca cagctcagac gaggccggcg
     9781 ggggcgcttc gacgatgggt gcataccgta gcgatacggc cggcgctacc tgggagacgt
     9841 atgtcgactt ccccactatt cagggtactt acggctggta tctttcaatt gtcggaattg
     9901 atccggccaa ccccgacatc gtgtttatgg gcggcgttac gctcgtgcgt tcgggcgacg
     9961 aggggatttg gcacaccgtc acacctccac atgtcgacat gcacgctatc gtctgggacg
    10021 cagccggccg cgtcgtggtg ggtgacgacg gcggcgtaca tcgctccgag gacggtggct
    10081 ggacgtggag tactatcaac gagggcctgg gattgatcca gggctacgcg ggattctcgc
    10141 ggcatcagca agagtactac atgttcatag gcacgcaaga caacggtacg cttcgccgca
    10201 acgcgaatgg cacttggacc aacatcctcg gcggcgacgg cggctggacg cagaccgacc
    10261 ctgtcagtac gtcgcgcgtc ttcgccgagt tccagggtac cgggaatctg tacagatcaa
    10321 ccagtctggg cagcagtttt acattcgtcg gtacgggcat cagttccgga gaccgaaact
    10381 gcttcatgcc gccgtacctg atcgatccca acgactccgc caaaatgctc tatgccacgc
    10441 atcgtattta ccggagtacg aattacggtt cgaactggac ggccatctcc tccgatctga
    10501 cgggcggagg cagcgccgcg gtccgctcgc tcgcgatggc cccttccgat ccctacaccc
    10561 tctatgcctc caccaatgac ggccgcgtgc tcgtttcgac caacggcggt tatacctgga
    10621 ccattatccg ggagaatgtg ccgggctggt cgcgcatcat gcgccagata tgcgtgcatc
    10681 cgaccgaccc tcagactgct tatgtggcgg tttcggcgtt cggagagcag caaatcctgc
    10741 gaaccacgga ccaagggcag acttggaccg cgctggacgg caatttcccc gacattcccg
    10801 ccaacgtgat cgccgtcgat atccgtacgc ccaggccaac tatcttcgtg ggcgccgaag
    10861 atggtctgta ccgttcggtg aacgatggcg tctcctggca ccgctacggc gccggcctgc
    10921 cacgctgccc cgtcatcgac ataagttttg aacctgaccg ctcacgcctg atcgtgtcta
    10981 cccagggccg cggcgtttgg agaattccga tcggcgtgcc ggctgacatg aatggcgaca
    11041 cgatggtcaa cgccttcgac atcgacgcct tcgtgctggc cttgaccgat ccggccgcct
    11101 tcgcggttca gtaccccacc gtcgacgtga ttgctaatgg cgacatcaat ggcgatggcc
    11161 tgctgaacgc gttcgacatt gacggctttg tgggcgtgct taccggagcg tgaacgcggg
    11221 cggagaaaac tcacatggat tcccgccggc cgaccggcgg caagcgccgc tggaggcgcg
    11281 gacccggacg gcggacgacg cccaccctgt gccgccggcg cgccgttgga cgcgctggcg
    11341 catggttaag tgggcggtgc gcgtgggcct gccgctgttc atcctgacct atgtgggatg
    11401 cgtgggcttg gacggcttct tctactaccc aaataagaaa acctacgatc ggcccgaaga
    11461 ctacaacttg cgatacgagg aggtgcgctt tcaaaccagc gacggtctga cgcttgccgg
    11521 ctggttcctg ccggctgtcc ggcataagcc cttccgcggc accattgtgc acttccatgg
    11581 caatgccgcg aacatcagcg gccacctgct gctcgtttat tggctcccgc tgcaaggcta
    11641 caacctgctg atgttcgact accgtggtta cggccagtct gaaggcaagc caacgcgggc
    11701 cggcacgatt gcagacggga acgcggccat cgactatgcg ctgagccgcg ccgatgttcg
    11761 cggggggccg ttgttcgtct atgggcagtc gctcggcgca gcggtggccg tagtcgtcgc
    11821 ggccgagcgc cccgaaatcg cgggggtggt ggccgaatcg ccgttcagcg gctaccggcg
    11881 gatcgcagcc cggcacttgc agaagcggat actaattccg ccgctggcca gaggtctggc
    11941 ggcgctatgc gtgtctggcg gttatgaccc ggtcgacgcc gtccctcgcc tggcgccgcg
    12001 gccgttgctg gtcattgtgg cgggcaatga caatatctgc tttccggatc tcggacgcga
    12061 gctgttcgac gccgcgtgcg agccgaagga aatttgggaa gcgccgggcg ccgaccactt
    12121 ggccattatg gaaggcaatg caaacgcatt gatggaccgc atcacggggt ttttcgatcg
    12181 tgcggccgag ggcagacgtt agctcggccg ccgatagtca aagcagtccc ggtacctgga
    12241 agtcggttgc cggcacgtcg ggcgcctgta cctgaattat cccgttttgg gccgtttccg
    12301 tggtgaacgt cagtaaggcc tgttcggtgg ccgcgcctgc cgccgacggg atgtcgaatt
    12361 gggctgaacc ctggaaggga attaggttca gcgttaatga aggatcggcg atggcgcccg
    12421 tcagcgtgat ctgagtaggc gggccgttct caacggtgaa gatcgccgag ttaatccgca
    12481 tttgcccacg ctgcataagc gtaacgtcga aattccccac cagggcgccg acggcggcgg
    12541 tattcacgct catggtgccc gccaggttcg tgccggccgg atcggaagag atcgtcaccg
    12601 gatcaagctg gccctgaatt gttccgccgt caaacaccag cgtcgtgaaa tcaatggttc
    12661 cgacgcgggt agtcagattc accctgctag tggtcaggcc gctgacgact cggtttgcgg
    12721 ttatcaaaga cgagcagccg gcgccgaagt caaccgcgat cagtgcgtta acccgggccg
    12781 cgtctgtgac gtacgagacc accggacact cgccaaagct ttcaatcgga ccgagcgtgt
    12841 cgagtgccag gcgggggtcg gcgaaggcgt tcagcgtgga aaagatttcg aagagggcct
    12901 cgatttgcgt catgcctgtc tgaatcgcct gctgctgtgc ggccgtcagg ctcggactga
    12961 cgttgtcgtt ggcgttctgg tttccaggct ggggaggaca gccggtcaat gcagcacaga
    13021 gggcgcaaaa aagcagcagg cagatgcagc gcgtcattag aagttctccg tagtcagagg
    13081 gccgtttgca caattatacc ggtgcaccag ccaggcacgc gagctgccgg cgaagatgca
    13141 acctgcgcca gggcgcttgt cggaggcggt cggtgtcgtc acagtgggtc gctttttcga
    13201 cgcggtcggt gtcatcagcg gaggcggtcg gttgctgctg gtgcgatgaa tctgtctcgc
    13261 gagtgggggt agttgtctca tgatacgccg tttggacgac gccggggcca gtctgccagt
    13321 ttgccaagct tggtcggcta tatcccataa aatcgtcggt gggtttgcgc caggggcagg
    13381 gagcccggct gccaaggtat gcgcgctcgg atttggcaaa gtgccgcgct gcgcgagagc
    13441 agga
//
LOCUS       DAOSYS010000081        13430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3876_length_13430_cov_5.392748, whole genome shotgun sequence.
ACCESSION   DAOSYS010000081 DAOSYS010000000
VERSION     DAOSYS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13430
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3876_length_13430_cov_5.392748"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..528)
                     /locus_tag="PLU99_06775"
     CDS             complement(217..528)
                     /locus_tag="PLU99_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOO16796.1"
                     /translation="MQVIADLCVVPLGVGLSVSKYVAACERVLAEAGLKTRLHAYGTN
                     IEGEWDQVFAAVKRCHEVVHEMGAPRVSTTLKVGTRTDRAQTMEDKVRSVQEKLAGPA
                     T"
     gene            943..1128
                     /locus_tag="PLU99_06780"
     CDS             943..1128
                     /locus_tag="PLU99_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOO16797.1"
                     /translation="MLNRIKQFLINEDGPTATEYAVMLALVIIVCIAGITLLGNTVNG
                     VFNTVSEGLPTGAPGGS"
     gene            1286..1840
                     /locus_tag="PLU99_06785"
     CDS             1286..1840
                     /locus_tag="PLU99_06785"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOO16798.1"
                     /translation="MTLGFWETTWAILVPGILYASWVDYSQRRVPNWLNATLAAAGLV
                     TQATHFGWSGVSAGLLGMMVGLGVLIVPWLMHGMGAGDVKLMAAIGCWLGPWLTLLSF
                     AAGSIIGGCVAIIMIVSSGRTAHALVNMQTIMTKVRSAETAFSEFGGARTFGATSQLL
                     PYGVPLTAGTLVVLLTYYLGGWLT"
     gene            1837..2235
                     /locus_tag="PLU99_06790"
     CDS             1837..2235
                     /locus_tag="PLU99_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HOO16799.1"
                     /translation="MRLTKGRKRWAAAAVEMAVVTPLLLTMLFGIIEYGWVFTVRQGL
                     TTAAREGARVAALPGSTDADIRERVASFLNPLGVTTYNVNITHATPSDATETVQVTVP
                     YADVTLIGSFFGSTDYDLGATCSMRKEGVD"
     gene            2486..3649
                     /gene="cpaB"
                     /locus_tag="PLU99_06795"
     CDS             2486..3649
                     /gene="cpaB"
                     /locus_tag="PLU99_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOO16800.1"
                     /translation="MNVKSFVPLIAGLCIGGLALKIGVSTLQRAKGAPSATVQLWAPA
                     AIVPRGTQITAEMLKAVQFPKDLVPPAAVREKDKLIGRVPHVDVPADLPILEPMLLPP
                     GSPAGVHVPPGLRAVAVKVDEGSGVDFHLEPGCHVDVIGYFQTQDGNRRETIARTLIE
                     DVQVAAVGARISPAGTETEGKTSRPARAVTLLVKPDAVPILHLAEQRGKIKLSMRGAD
                     DAQEIGPGRTVSDSELLAGPAKGAHNDQKDKEQSGPVGGWLSKLFARSPEPAEAAAEP
                     AAPAPAAVPPAPANPPWVVQVYRGNQVETIQFQSRDSRERVESQRDPLTGSATSGSAA
                     IQAGQSPQLALALSPGGRGKAAPAGEEASGEETKARPESDLQAQDQVPQEPSE"
     gene            3646..5187
                     /locus_tag="PLU99_06800"
     CDS             3646..5187
                     /locus_tag="PLU99_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HOO16801.1"
                     /translation="MIRNQTITKSRFPSYFWTAVAAFAAVAATCPSLCAGPDGPVDFT
                     VTQLPHTGKLISVAVNKGVLVDFNVPLREVRIANPDIADATPTGPKQILINGKSFGAT
                     QLVALLDGGEQRIFDVAVDLDMERLQASIRSAVPHAQVRASSVLDTVVLTGTVPDAES
                     AQKLAEIAEIYAKRVVNHLRVAGVQQVVLRCTVAEVNRTATRQLGFNGWIAGDNIRDV
                     FAVSQLGGINPVNIGAAGGVDVTGRIPFATDQNGLNLTPRPTLSFGFPRVQMQVFIQA
                     LRENGLLRVLAEPNLIAISGQEAEFLAGGEIPIPVPQGMNQSITIEYREYGVKLRFTP
                     AVMGPNAIRLKVAPEVSEPDYATAVTISGYVVPGLTQRRVETTVELAGGQTFAIGGLL
                     SERVRGVARKVPALGDVPVLGALFSSVDYQSQETELVVLVTPELVAPLSPDQIAYVPG
                     AEMVNPNDCELFFGGQLEGQPARATCEPEPGCDGPGPVKTAEVFGPAVRGLRGPVGPA
                     GEEGG"
     gene            5415..7124
                     /locus_tag="PLU99_06805"
     CDS             5415..7124
                     /locus_tag="PLU99_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO16802.1"
                     /translation="MQVAVLSGVIMGFAALAIDVGMLYTSKAELQAACDSAALAAAAQ
                     LSAEGDTAELAREAAAEYAARHKILGDAAQLDVNRDVEFGRAAFNPVTNRFEFQPSEF
                     SYDAVRVTVRRTADSPGGSIPLLFANSMGVSKKDMWARATAVLVPRDIAVVIDLSNSM
                     SYDSQLRFWDRADGGSSNLRDVWAALNGPEPDRPYLPGPETETEYAADTGPTFGAMTT
                     WGDPLVPRSYKPASDPGLWYIPKGKNTSVPAAEASLAARGYSAAERSALLSGAYDGTA
                     SYWVNRTGVILGLALWHSGKSGGLSPSGGDGDNYVEDKEVEWIPIPSFSSGWTWKDYI
                     KGVGGQTSQKPTADGFAYRYGLKTLVDYILQYHPDISRTDNLWATPAEPLRATKDAVQ
                     TMINVITALNSMDHLSLEVFATTAHHQVDLTDNLQSIADTLYQRQAGHWDTNTCIGGG
                     LACAINELKSSRARAASAKVIVLMSDGVPNVDEAGRVLDFGASAAVQYAYEQADRAGR
                     EGIRIYCVSVGYSVDRSIMQTIAAKAHGQEFFASGSPEEYTEQLEDIFRALGGKRPVA
                     LIE"
     gene            7149..8339
                     /locus_tag="PLU99_06810"
     CDS             7149..8339
                     /locus_tag="PLU99_06810"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOO16803.1"
                     /translation="MSGRRGLKMYSLERTRLSRPRRRAAVAAQVAVFAGVMMGFGALA
                     IDLGRLYTARGELQRAADSAALAGVSSYFSDAGLLQDAPALGTMARSRAGTLALRNPT
                     LGAYTQLESPDLVLGTLDVSDPHSVLDTSGGARLNAVQIMVRRTEGSRNGPVDFLFAH
                     IFGRAHGGVVASATAVVDDRFAGFRTTEINQILLPFTIHRDYYEDMLVSGGDQWSFED
                     DRLVTTSDGVPEIKLFPWKLSGHSSPEGAGNFGALNIGDDDSGLPPIAWQILHGISAE
                     QLERTLGTSTLIFYDAFGNPVAYEIPGTPGMKAAMEDELHQREGDVVAFFLHDAYWDS
                     GSQCTYRIVDIRFGRVMHVDLTGSPKNKALLLQPVAYTGPGVIVSEHAPSSQGAAGYA
                     RLVR"
     gene            8356..9528
                     /locus_tag="PLU99_06815"
     CDS             8356..9528
                     /locus_tag="PLU99_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOO16804.1"
                     /translation="MKGKTRQNLRRAGVAVQVAVFSGVMIAFGALALDLGRLYTARGE
                     LQRAADSAALAGASAIFTDAGLIRDDARVDHLAASRASEITLQNPTLGSGTILDSPDL
                     LIGALDLNDPNAVLDTSGVNRFNAVQVLLRRAPDSSNGPVSYLFAHIFGQHQGSVAAS
                     ATAVVDDRFAGYRTTESNRLLTPFTIHVERYAECLADGADDWSFDGEDVVAMSDGVRE
                     IRLFPWKLSGGGKKGGDGSTSDGAGNFGILNVGTGHGVPAVEEQILHGITPEQLLAEV
                     GTSELIFYDPFGEPVTYVMGGTPGMKSGMEDALEARIGELVGFFLHDSYSGTGANLTY
                     NIVNIRFGRVMEVNLHGNPSDKRLIIQPVAYTGPGIVIKEYAKSSEGAAGLVRLVR"
     gene            9688..10941
                     /locus_tag="PLU99_06820"
     CDS             9688..10941
                     /locus_tag="PLU99_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16805.1"
                     /translation="MSQVIRVIVVNADEAATVELRNHLLGVEGVKIVAEIDEPALLPQ
                     ALAQFPAEVLLIHLDPDPAGMMDAVAPLVEARKEHLAAIGMTEDRDAELVVRAMRAGM
                     REFLWKPFPPEQLSEILLRVAGEAAGAGRRLGRLIPVVGTCGGVGATTLVTNVGVELA
                     QLRDREWSAPSGAGSAPSVAVVDLDFRYGQVAMFLDAQAKYSVAELCDTPERIEPQMI
                     ERVMYRHPTGVHVLAQPNDLERAERISAAQVGSVLSALQEHYDFVIVDGPVRFDPTAR
                     TVLDMTDTCLLLLQLVVPSVRNADRMLHEMARCGYNLDRVRIVCNRFGREAGYLEQAD
                     VETTLGRKICWTVPDDWKTSSTAVNVGTPLLEWAPKSKLRAAYRAIALALASATESAA
                     GEKPEAGSAEPARKSLRSIFAGQKS"
     gene            11152..12426
                     /locus_tag="PLU99_06825"
     CDS             11152..12426
                     /locus_tag="PLU99_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOO16806.1"
                     /translation="MRFQELKASIHRKLVEQIDMTKLAREVSEELRQQVKQMVKALCE
                     EENTLLNFSERERLAQEILDETFGLGPLESLLADPHVSDILINGPKRVYVERGGRLEL
                     TNVTFKDNGHLLHVIDKIVAPLGRRCDEVCPMVDARLKDGSRVNAIIPPLAIDGASVS
                     IRRFGKDPITWDDYIAFKSCAPEMVDFLRACVQSGLNILIVGGTGSGKTTLLNNLSSF
                     IPEDERIVTIEDAAELQLRQPHIVRLETRPANIEGKGRITIRDLLINALRMRPDRIVV
                     GECRGGETLDMLQAMNTGHEGSMTTIHANSTRDAVQRVETMVMMAGFELPTRAIRQQF
                     SSAINLIVQAARLTGGPRKITAITEVQGMEGDVITMQDIFKFEQDGVNSDGKAFGRIV
                     ATGVRPQFVGRLVSAGTEVDPSWFEPRDLITD"
     gene            12431..>13430
                     /locus_tag="PLU99_06830"
     CDS             12431..>13430
                     /locus_tag="PLU99_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO16807.1"
                     /translation="MTCTLLALKIMESASSVLFILAPLLGGVMLAYGVFQVALDLRSN
                     PRKRVMDRLKANRERQEANHGFQDFRRQTAEATGMLAKAFTRFSFTERFQRMLDQANV
                     PWSAAQTLVRLSVTAAVLLAATLAFGIPPLAGLGMAAAVYILPILYLMRRRKKRLARL
                     VEQLPDVFELLSQSLRAGHSLASGMQLISRELPEPAGIEFGRVFHEQNLGLKIEDALR
                     NMAERVDMLDVRFFVTAVLIQRQTGGDLAEVLDKIGAVIRERIKLFGTVQALTAEGRL
                     SGYVLLALPVLMFAVMLQVNRDYATMLLYEPAGKMMLTVAVVMQLMGWAMIKKIVNI"
BASE COUNT         2488 a         3970 c         4623 g         2349 t
ORIGIN      
        1 ggcgcggatg tattacgaat ggagagcgcg acggaatgcg agtgcccgcg aagcagggtg
       61 accagcatgc tggtgcgggc atgatcggcg cactaaggac gggtggcgtc ggagcggcac
      121 gctctaacct tctcgacctt ggcccgactt cttcgagccg gcgccgtgaa gcagattgaa
      181 atcgcgcctg gcatttgcag gccggttcaa gcgcggctag gtcgcggggc cggcgagttt
      241 ttcctggacg ctacgaacct tgtcttccat ggtctgggcc cggtcggtcc gcgtgccgac
      301 cttgagagtg gttgaaacgc gcggggcgcc catttcgtgt acgacttcgt ggcagcgttt
      361 gacggcggcg aaaacctggt cccactcgcc ttcgatgttg gtcccgtagg cgtgcagccg
      421 cgttttgagg cccgcctcgg ccagcacgcg ctcgcaggcc gccacgtact ttgaaactga
      481 caacccaact cccagcggaa cgacgcacag atcagcaatc acctgcatcc tactctccca
      541 agcagaagtt cgggtcccgc gacccgacct cacggcagca tgaatatttt ccggactaat
      601 cattctattc ggcccaacac gccgggtaag gccccagata accatggggc cggccgcagg
      661 ggccgcaatt cgcgaaattt ttttgcacgg gccgggaacg ttttcgaaac ttggattggc
      721 gtcttccgcg aaagcgggag aaagccaact caggaatgag tgcctcgggg ggaccgggcc
      781 gaacgaggcc actgacgtta atcggggttg gcagacgcga caccggcgag gcgccggtgc
      841 cacaaacccc gcaggtgcct ccggagcgga atccggaagg agttccaagg cacggaagcg
      901 gctgtcgcaa cagggtttgc tgcgtagcaa aaaggagaag gtatgctgaa tcggatcaaa
      961 caattcctga tcaacgagga tggtccgacc gcgaccgaat acgcggtcat gctggcgctg
     1021 gtgatcatcg tctgcattgc gggcatcaca ctgttgggca acaccgtgaa tggcgtattc
     1081 aacacggtgt cggaagggct gcccacgggc gcgccgggtg gcagctaatc tgaggcgacc
     1141 gcgtcgtcgc ggttgagaag gtgcggacgg cggcggtcgc tcacggcgac gtcgccgtcc
     1201 gtgtttctag cggccaagtg taaagacggg cgcggcagca gctccagcgc cgcttatggg
     1261 acacaacaga aaaggaagcg caagaatgac tttgggattt tgggaaacaa cctgggccat
     1321 cctggtcccc ggcattctgt atgcgtcgtg ggttgactac agccaacggc gtgttccgaa
     1381 ctggctgaac gcgacattgg ccgcggccgg tctggtgact caggccacgc atttcggctg
     1441 gagcggcgtc agtgccgggc tgctggggat gatggtgggg ctgggcgtgc tgatcgtgcc
     1501 ctggctgatg cacggcatgg gggccgggga cgtgaagctg atggcggcga tcgggtgttg
     1561 gctgggccca tggttgacgc tgctgagctt tgcggccggc agcattatcg gcggttgtgt
     1621 tgcaatcatc atgattgtct caagcgggcg gactgcgcac gccctggtga acatgcaaac
     1681 catcatgacg aaggtgcgca gcgccgagac cgcgttcagc gagttcggcg gagcgcggac
     1741 cttcggcgcg actagtcagc ttctgccata cggcgtgcca ctgacggcag ggacgctggt
     1801 ggtattgctg acttactacc ttggagggtg gctaacgtga gactcacgaa aggaaggaag
     1861 cgctgggcgg cggccgccgt ggagatggcg gtggtgacgc ctctgctgct gacgatgctg
     1921 ttcggcatca tcgaatacgg ctgggtattc accgtccggc agggtcttac gactgcggcc
     1981 cgcgaggggg cgcgggtcgc ggccctgccg ggttcgacgg atgcggacat tcgcgagcgg
     2041 gtggccagct ttttgaatcc gctgggcgtc acgacctata acgtgaacat cacgcacgcg
     2101 acgccctcgg acgccacaga gacggtgcag gtgacggttc cgtacgccga cgtgacgctg
     2161 atcggcagct tctttggcag taccgactac gacctcggtg caacctgttc gatgagaaag
     2221 gaaggggtgg actaagcggc gccggcaggg ccgttgtgga gcaagcggcg ctagctggtg
     2281 cagtgccgca gatgccgcac tgcacggagc ggagctgcgg taaagcgcga tttcgaaatc
     2341 tcacgtttga gatttcaaat cgtggctcgc ggatgggaag gacgcgcgcc gcggcgcggc
     2401 agttcgcgcc gggcaacgag gcaggccgcc caagttcagg gcggcccggg tgcgcgcccg
     2461 gaaatagcgc ccgaggtgtc gggaaatgaa cgtcaagagc tttgtgccac taatcgccgg
     2521 cctctgcatc ggagggctgg cactcaagat tggcgtcagc acattgcagc gcgccaaggg
     2581 ggctccctct gccactgtac aactgtgggc gcccgcggcg atcgtgccgc gcggcacgca
     2641 gatcacggcg gagatgctga aggccgtgca gttcccgaag gacctcgtcc cgccggccgc
     2701 ggttcgcgaa aaggacaagc ttattgggcg ggtgccgcac gtcgatgtcc cggcggatct
     2761 gccaatcctc gaaccgatgt tattgccgcc cggctcgccg gcgggcgtgc acgttccgcc
     2821 aggactgcgg gcagtggcgg ttaaggtgga cgaaggctca ggcgtggact ttcacctcga
     2881 gcctggctgt cacgtggacg tgatcggcta cttccagact caggacggca accggcgcga
     2941 gacgatcgcc cgcacgctga tcgaggacgt gcaagtggcc gccgtggggg cgcgcatcag
     3001 cccggccggg acggagactg aaggcaagac cagtcgtccg gcacgcgcgg ttacgttgct
     3061 ggtgaagccg gacgccgtgc cgattctgca tttggcagag cagcgtggaa agatcaagct
     3121 gagcatgcgc ggcgcggatg acgcgcagga gatcggcccc ggccggacgg tcagcgactc
     3181 cgaactgctg gctggcccag cgaagggcgc ccacaacgat caaaaagaca aggaacagtc
     3241 cggcccggtt gggggctggc tgagcaagct tttcgctcga tcgccggagc cggccgaagc
     3301 cgcggcggaa ccagccgcac ccgcgccggc cgccgttcca ccggcgcctg caaatccgcc
     3361 gtgggtggtg caggtgtacc gcggcaacca ggttgagacg atccaattcc agagccgcga
     3421 ctcacgcgag cgcgtagagt cgcagcgaga cccgctcacg ggttcggcga cgagcggtag
     3481 cgctgccata caggcgggtc aatctccaca gttggcgcta gctctcagcc cgggtgggcg
     3541 cggaaaagct gcgccggccg gtgaggaagc atcgggggag gaaacgaaag cgcgtcccga
     3601 atcggacttg caggcccagg atcaggtgcc ccaggagccc agcgaatgat ccgcaatcaa
     3661 actattacca aaagccgttt ccccagttac ttctggactg ccgtggctgc atttgcagcg
     3721 gtcgccgcga cgtgcccctc gctatgcgcg ggcccggacg gccccgtgga cttcacggtg
     3781 acgcagttgc cgcacaccgg caagctgatc agtgtcgcgg tgaacaaggg ggtgctggtg
     3841 gatttcaatg taccgctgcg tgaggtgcgg atcgcgaatc cggatattgc agacgcgacg
     3901 ccgaccgggc cgaagcagat cctgatcaac ggcaaatcgt tcggggcgac gcagctcgtg
     3961 gcactgctgg acggcggaga gcagcgcatc ttcgacgtgg cggttgatct cgatatggag
     4021 cggctgcaag ccagcattcg ctcggcggtg ccgcacgccc aggtgcgggc gagttcggta
     4081 ctcgacacgg ttgtgctgac cggcacggtg ccggacgccg aatcggccca gaagctggcg
     4141 gaaatcgcag aaatctatgc gaaacgcgta gtgaaccatt tgcgcgtggc gggcgtgcag
     4201 caggtcgtgc tgcgctgcac ggtggccgag gtgaatcgca cggccacgcg gcagcttgga
     4261 ttcaacgggt ggatcgccgg cgacaacatc cgcgacgtgt tcgccgtcag ccagttgggc
     4321 gggattaacc cggtgaacat aggtgcggca ggcggagtgg acgtgaccgg gcggattccg
     4381 ttcgccacgg accagaacgg cttgaacctg acgccgcggc ccacgctgag ctttgggttc
     4441 ccgcgcgtgc aaatgcaggt cttcattcag gcgctgcgcg agaacgggct gctgcgcgtt
     4501 ctggcggagc caaaccttat agccatcagc gggcaggagg cggagttcct ggcggggggc
     4561 gagattccca tcccggtgcc gcagggcatg aaccaatcga tcacgattga ataccgcgaa
     4621 tacggcgtga agttgcgctt cacgccggcg gtaatggggc cgaacgccat ccgcctgaag
     4681 gtcgcgccgg aggttagcga gcccgactac gcgacggcgg tgacgatatc gggctatgtg
     4741 gtgccggggc tgacccaacg gcgggtggag acgacggtcg agctggccgg cggccagacg
     4801 ttcgcgatcg gcggtttgct gagcgagcga gtgcgcggcg tcgcgcggaa ggtgccggcg
     4861 ctgggcgatg ttccggtgct gggggcgctg ttcagctcgg tggattacca gtcgcaggaa
     4921 actgagttgg tggtgttggt gacgcccgag ctggtggcgc cgctatcacc ggaccagatc
     4981 gcatacgtgc cgggggcgga gatggtaaat ccgaacgatt gcgagttgtt cttcggcgga
     5041 caactcgaag gacagccggc gcgcgcgact tgcgagcccg agcccggctg tgacggcccg
     5101 ggcccggtaa agacagccga agttttcgga ccggcggtgc gcggcctgcg tggccccgtt
     5161 gggccggccg gtgaagaagg cgggtgatca ggagcagcgg cggaccgaaa aaaaacgaag
     5221 cgcgcggact gcgcggccgg cggcgagagc ggccggccgc cggtccgaca agcttttcag
     5281 atgcggaagg cggccgggcg cgagctcggc ctggcctatg tcacaactgg ctggtgatgg
     5341 gaggacgaaa gatgtctgga gagaaggtga ggaaggttgt aggtcggcgc tggcgacgac
     5401 gcggcgctgt gaccgtgcaa gtggcagtgc tgtcgggcgt cattatgggg ttcgcggcac
     5461 tggcgatcga cgttggcatg ctctacacgt ccaaggccga attgcaggcc gcctgtgact
     5521 cggctgcgct ggccgcggcg gcccaactga gcgccgaggg agacacggcg gagttggcgc
     5581 gtgaagcggc ggccgagtat gcagcgcgtc ataaaatttt gggggacgcc gcacaactcg
     5641 acgtgaaccg agacgtggag ttcgggcggg ctgcgttcaa cccggtaacg aatagattcg
     5701 agttccagcc ttcggagttc agctatgacg ccgtgcgcgt gacggtgcgg cgcacggctg
     5761 attcgccggg cggatcgatt ccgctcttgt ttgcgaactc gatgggcgtc agtaagaagg
     5821 acatgtgggc ccgcgcgacg gcggtgctgg tcccgcgcga tattgcggtg gtcatcgact
     5881 tatccaattc gatgtcctat gacagccaac tgcgtttctg ggaccgcgcc gacggtggta
     5941 gcagcaactt gcgcgacgtg tgggccgcct tgaacgggcc cgagccggat cgtccatact
     6001 tgccgggacc ggaaaccgaa actgagtatg cggccgatac cggacccacg tttggcgcaa
     6061 tgactacttg gggcgacccg ctggtgccgc gcagctacaa gcccgcctct gatcccggct
     6121 tgtggtacat acccaaagga aaaaacacct cggtgccggc cgcggaagcc agtctggcgg
     6181 cgcgcggcta cagcgcggcg gagcgctcgg cactgctttc gggcgcctat gacggcaccg
     6241 cgagctattg ggtgaaccgc accggcgtca ttctcggact ggccctctgg catagcggca
     6301 agagcggtgg tctgagtccg tccggcggcg acggggacaa ctacgtggaa gacaaggaag
     6361 tggagtggat tccgataccc agtttttcca gtggttggac ctggaaggat tacataaaag
     6421 gcgtgggcgg ccagacgagt cagaaaccga ccgcggatgg gttcgcgtac cgctatggcc
     6481 tgaagaccct ggtcgattac atccttcagt atcatccgga cattagtcgg acggacaatc
     6541 tgtgggctac gcctgcagaa ccgctgcgtg cgaccaagga tgccgttcag accatgatca
     6601 acgtgatcac ggctttgaac agcatggacc atctgtcgct ggaggtgttc gcgaccacgg
     6661 ctcaccacca ggtcgatttg acggacaatc tgcagtccat cgccgacacg ctttaccagc
     6721 gtcaggcggg ccattgggac acgaacactt gcataggcgg cggcctggcc tgcgccatca
     6781 atgagctcaa gtcctcgcgc gcccgcgcgg cgtccgccaa ggtgatcgtg ctgatgtctg
     6841 acggcgtgcc gaacgtcgac gaagccggcc gcgttctcga cttcggggcc tcggcggcgg
     6901 tgcagtacgc ctacgaacag gcggatcgag cgggcaggga agggattcgg atttattgcg
     6961 tcagcgtggg ctacagcgtg gaccgcagca tcatgcagac catagctgcc aaggcgcatg
     7021 gtcaagagtt cttcgcgtca ggctcgcccg aagaatacac ggaacaactg gaggacattt
     7081 tccgggccct gggtggaaaa cgccccgtgg cgctgatcga gtaggcgagg gatggagtaa
     7141 gcggccgtgt gagcggcagg agagggttga agatgtattc tctagagcgg acgcggttga
     7201 gtcgcccgcg gcggcgtgcg gccgtcgcgg ctcaggtggc ggtgtttgcg ggggtgatga
     7261 tgggcttcgg cgcattggcc atcgacttgg gccgtctcta tacggcgcgc ggcgagctgc
     7321 agcgggccgc tgattcggcg gccttggccg gtgtgtcgag ttacttcagc gatgcgggtc
     7381 tgttacagga cgcgccggcg ctgggcacga tggccagatc gcgtgcgggt acgctggcat
     7441 tgcgcaatcc gacgctcggg gcctatacgc agcttgaaag ccctgacttg gtgctgggca
     7501 ccttggatgt atcagacccg cattccgtgc tggacacgtc gggcggggca cgcttgaacg
     7561 cagtccagat catggtgcgc cgcaccgaag gcagccgcaa cggaccggtt gacttcctct
     7621 ttgcgcacat tttcggacgc gcgcacggtg gtgttgtggc ctcggcgacg gcagtcgtgg
     7681 atgaccgctt tgccggcttc cgcacgaccg aaatcaacca gatactgttg ccgttcacga
     7741 ttcaccgcga ttactacgag gacatgctcg tcagcggcgg cgatcaatgg tccttcgagg
     7801 acgatcgcct cgtcaccacc tcggatggtg tgcccgagat caaactgttc ccctggaagc
     7861 tcagcggcca ttcgtcgcct gaaggcgccg gcaacttcgg cgcgctcaat attggcgacg
     7921 acgattcggg gttgccaccg atagcgtggc agatcctaca tggcatttcg gccgagcagc
     7981 tagagaggac gctgggaacc tccactttga tcttctacga cgccttcggc aacccggtcg
     8041 cctatgagat tcccggcacg cccgggatga aggcggccat ggaagacgag cttcatcagc
     8101 gcgagggcga cgtggttgcc ttcttcctgc acgacgcgta ttgggacagc ggcagccaat
     8161 gcacgtaccg cattgttgac attcgcttcg ggcgcgtaat gcacgtggac ctgacgggca
     8221 gtcccaagaa caaagcattg ttgctgcagc ccgttgcata caccgggcct ggcgtgatcg
     8281 tcagcgagca cgcgccctcg tcccagggtg cggccgggta cgcacggctg gttcgataac
     8341 tcggcggagc agggtatgaa gggcaaaacg cggcagaatc tcaggcgcgc cggtgtggcg
     8401 gttcaggtgg ccgtcttttc cggagtgatg atcgcgtttg gcgcgctggc cctcgatttg
     8461 gggcggctct acaccgcccg gggggaacta cagcgcgccg ccgactcggc ggcgctggca
     8521 ggcgcctcgg cgatcttcac tgatgcaggt ctgatccgcg acgacgcgcg agtggaccac
     8581 ttggccgcca gccgggcctc ggaaatcacg ctccagaacc cgacgttagg cagcggtact
     8641 attctggact cgccggattt gcttatcggt gctttggacc tcaacgaccc gaacgcagtg
     8701 ctggacacgt caggcgtgaa ccgctttaac gcggttcagg tgctgctgcg gcgcgcgccg
     8761 gactcctcca acggcccggt cagctatctg ttcgctcaca tttttggtca gcatcagggt
     8821 agcgtcgccg cttccgccac ggcggtcgtg gatgatcgct ttgccggcta caggaccacc
     8881 gagtccaacc gcctgctaac ccccttcacc atccatgtcg agcggtacgc cgagtgcctt
     8941 gccgacggtg cggacgactg gtcctttgat ggggaagacg tcgtggctat gagcgacggc
     9001 gtccgagaaa tccggctgtt tccttggaag ctcagcggcg ggggaaagaa gggcggtgac
     9061 ggcagcacgt ccgatggtgc cggtaatttc ggcatcctga acgtcggtac ggggcacggt
     9121 gtgccggccg ttgaagagca aatcctgcac ggcatcacgc cggagcaact tctggcggag
     9181 gtcggcactt cggaacttat cttttatgac ccgtttggcg aaccagtgac gtacgttatg
     9241 gggggcacgc cgggcatgaa gagcggcatg gaggacgcgc tagaggctcg catcggtgag
     9301 ttggtcgggt ttttcctgca cgactcatat agcgggaccg gggccaacct gacctacaac
     9361 atcgtgaaca tacggtttgg gcgcgtgatg gaagtgaatc tgcacggtaa tccgtccgac
     9421 aagcgattaa ttattcaacc agtcgcctac accgggccgg gcatcgtcat caaggaatac
     9481 gcaaaatcca gcgaaggcgc agcgggtctg gttcggctgg tccgttaacg gcgcgggctg
     9541 aaatgagtaa ccgcgcgaaa cagccgattc cataagcatg tagcgcctga tccggcgtac
     9601 gtgtcgccgg cgcgatgcta gcagccggat ccgaggtttc tcggacctgg gcggaccggg
     9661 cgaaggcata caaggataca cgcagctttg agccaggtta ttcgagtcat cgtcgtcaat
     9721 gccgacgagg ccgccacggt cgaactgcgc aatcatcttc tgggcgtgga gggggtgaag
     9781 atcgtggccg agatcgacga gccggccctg ctgccgcagg cgttggcgca gttcccggcc
     9841 gaagtgctgc tgattcatct ggaccccgac ccggccggca tgatggacgc ggttgcgccg
     9901 ctggtagagg cgcgcaagga gcacctggcg gcgatcggca tgaccgagga ccgcgacgcg
     9961 gagctggtgg tccgggccat gcgggccggg atgcgcgagt ttctatggaa gcccttcccg
    10021 ccggagcagt tgagcgagat tctgttgcgg gttgcggggg aggcggcggg tgcgggccgg
    10081 cggctggggc gattgattcc tgtggtcgga acgtgcggcg gggtgggggc gacgaccctg
    10141 gtcaccaatg tcggcgtgga gctggcgcag ctccgcgacc gcgagtggtc cgcgccgagt
    10201 ggggcgggca gtgcgccaag cgtcgcggta gtggacctgg acttccgcta tgggcaggtc
    10261 gcgatgttcc tggatgcgca ggcgaaatac agcgtggctg agctatgcga cacgcctgag
    10321 cgcatcgagc cgcaaatgat cgagcgcgtg atgtaccggc acccgaccgg cgtgcacgtg
    10381 ctggcgcagc ccaacgacct ggagcgcgcc gagcgaatta gcgccgcaca agtcggcagc
    10441 gtgctgtcgg cccttcagga gcactacgac ttcgtcatcg tggatgggcc ggtgcgcttc
    10501 gatcccaccg cgcgcaccgt gctggatatg accgacacct gcttgttgct actgcagctc
    10561 gtcgtgccgt ccgtgcgcaa tgcggaccgg atgctgcacg agatggcccg ctgcggctac
    10621 aacctcgacc gcgtgcgcat cgtctgcaac cgcttcgggc gcgaggcggg ttacctcgaa
    10681 caagccgacg tcgaaaccac gctcggccgc aagatctgct ggaccgtgcc ggacgactgg
    10741 aagacctcga gcacggccgt gaacgtgggc acacccctgc tggagtgggc cccgaaatcc
    10801 aaactgcggg ccgcctatcg ggccatagcg ctggccctgg cgagcgccac cgagagcgcg
    10861 gccggagaga agccggaagc gggctcagcc gaaccggccc ggaaaagtct gcgctcgata
    10921 tttgccggac agaagtcata gcgaggaatc gcacagggag tgcatatggc gttctttgcg
    10981 aaaaaacctg cggctactac ggcggcgccg ccgccatcca gcgaaactca atcgggacac
    11041 accggcgttc agaccaagac tccggccaag cctgcccggg aagccgcagc ggatgccaaa
    11101 ccgccgcagc gcccggggcc tgtcagggat cgcataaccg acgagcgcgc catgcgcttt
    11161 caggagctga aggcctccat tcaccgcaag ctggtcgagc agatcgacat gaccaagctc
    11221 gcccgcgagg tcagcgagga actgcgccag caagtgaaac agatggtcaa agccctgtgc
    11281 gaagaggaga acacgctcct gaacttcagc gagcgcgaac ggctggctca ggaaatcctg
    11341 gacgagacgt tcggcctcgg gccgctggaa tcactgctgg ccgacccgca cgtgagcgac
    11401 attctgatca acggtcccaa gcgggtctac gtcgagcgcg gcgggcggct ggagctgacc
    11461 aacgtgacct tcaaggacaa cgggcacctg ctgcacgtga ttgacaagat cgtggcgccg
    11521 ctgggccggc gctgcgacga agtctgcccg atggtcgatg cgcgcctcaa ggacggcagc
    11581 cgcgtcaacg cgattattcc gcccctggcg atcgacggcg cttcggtctc gattcgccgc
    11641 ttcggcaagg accccatcac ctgggatgac tacatcgcct tcaagtcctg cgccccggag
    11701 atggtcgact tcctgcgggc ctgcgtgcag tcggggctga acatcctgat tgtcggcggc
    11761 accggctccg gtaagacgac tctgctgaac aacctctcgt ccttcatccc ggaggacgag
    11821 cgtatcgtga ccatcgagga cgccgccgaa ctccagttgc gccagccgca catcgtgcgt
    11881 ttggaaaccc gtccggccaa cattgagggc aaaggccgca tcacgattcg cgacctgctc
    11941 atcaacgccc tgcgtatgcg gccggaccgc attgtggtgg gcgagtgccg cggcggcgag
    12001 acgctcgaca tgttgcaggc catgaacacg ggccacgaag gttcgatgac cacgatccac
    12061 gccaacagta cgcgtgacgc cgtgcaacgc gttgagacca tggtgatgat ggccggtttt
    12121 gagctgccga cgcgcgccat ccgccagcag ttctcctcgg ctatcaatct gatcgtgcag
    12181 gcggcgcgcc tgacgggcgg cccgcgcaag atcaccgcga ttaccgaggt tcagggcatg
    12241 gaaggcgacg tcatcaccat gcaggacatc ttcaaattcg aacaggatgg cgtgaactcc
    12301 gacggcaagg cctttgggcg gattgtggcg accggcgttc gcccgcaatt cgtaggccgg
    12361 ctggtttcgg ccggcaccga ggtagacccg agctggtttg agccgcgcga cctgattacg
    12421 gactgatgcc atgacatgca cacttctggc tctgaagatc atggagagcg cgagcagcgt
    12481 tctgttcatc ctggccccgc tgctgggcgg cgtgatgctg gcttacgggg tcttccaggt
    12541 ggcgcttgat ctgcgctcca atccgcggaa gcgcgtcatg gatcggctga aggcaaaccg
    12601 cgagcgccag gaagccaacc acggattcca ggatttccgg cggcaaaccg cggaggccac
    12661 cggcatgctg gccaaagcct tcacgcgctt cagtttcacc gagcgctttc agcgcatgct
    12721 cgaccaggcc aatgtgccct ggtcggccgc ccagaccctg gtgcgcctgt cggtaacggc
    12781 agccgtattg ctggccgcta cgctcgcctt cggcattccg cctctggccg gcctggggat
    12841 ggccgcggcg gtttacatct tgcccatcct gtatctgatg cgaaggcgca agaagcgcct
    12901 ggcacggctg gtcgagcagc ttccggatgt gttcgagcta ttgagccagt cgctgcgcgc
    12961 cggccattcg ctggccagcg gcatgcaact gatttcgcgc gagctgccgg aaccggccgg
    13021 catcgaattc ggccgcgtgt ttcacgaaca gaacctgggc ctgaagatcg aggacgcact
    13081 gcgcaacatg gccgagcgcg tcgacatgct cgacgtgcgg ttcttcgtta cggcggtgct
    13141 cattcagcgc cagaccggcg gcgatctggc cgaagtgctc gacaagatcg gggcggtcat
    13201 ccgcgaacgc atcaagctct tcggcaccgt gcaggcgttg acggccgaag gacgcctctc
    13261 gggctatgtg ttgctggcgc tgccggtgct gatgttcgcc gtgatgctgc aggtgaatcg
    13321 cgattacgcg accatgctgc tgtatgagcc ggccggcaag atgatgctaa ccgtggcagt
    13381 ggtcatgcag ctaatggggt gggcgatgat caagaaaatc gtcaacatca
//
LOCUS       DAOSYS010000082        13245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3936_length_13245_cov_5.484458, whole genome shotgun sequence.
ACCESSION   DAOSYS010000082 DAOSYS010000000
VERSION     DAOSYS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_13245_cov_5.484458"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLU99_06835"
     CDS             complement(<1..346)
                     /locus_tag="PLU99_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOO16808.1"
                     /translation="MELNTIYCADSCQLLESWEPGCVDLVFADPPFNIGYVYDKYHDD
                     LPNAEYVAWTKRWMAACQRVLKPTGSFFIAIGDEFAADVRIVGRQLGLHLRNWIIWHY
                     TFGQNMKGKFCRS"
     gene            complement(428..1006)
                     /locus_tag="PLU99_06840"
     CDS             complement(428..1006)
                     /locus_tag="PLU99_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOO16809.1"
                     /translation="MNAENDKDALRLPCDASAVACDANEALRVACDAMCGGLARWLRV
                     LGVNTAYVRDIEDSALVRQALAERRVVLSSDRKLFERRVFTTGELPGLFIPVGLRLAD
                     QVRFVAGRLKLPVGFPRCTVCNGELEPVDRAAVADVVPARSLIWVREFYRCRECRQVY
                     WEGTHWRRIGALRAEIVNMRASGSAPETTPQK"
     gene            complement(1142..1651)
                     /locus_tag="PLU99_06845"
     CDS             complement(1142..1651)
                     /locus_tag="PLU99_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16810.1"
                     /translation="MTRPALTLIEAIIVIMIIGLLAVLAVPRFSQAEPGPQTLNPRPA
                     LVTLRNAIEMYYYDHGVYPGQHSDGIHPPGSAEAFVAQLTSFSDAEGRVSPEKSNQAC
                     FGPYLRDGIPACPVLPRLGKSGVVMTTDPPAFQEWAADAGWVYNCQSGAIAVNSNARD
                     ANNVCYDRY"
     gene            complement(1623..>3113)
                     /locus_tag="PLU99_06850"
     CDS             complement(1623..>3113)
                     /locus_tag="PLU99_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO16811.1"
                     /translation="TGSSLAAFVGLSSLILCLLGGVAGAAGKQQRCQSNPRGAISASA
                     SHAAAAVPPGGTAQRRRFMYASANMANDFCAWVDSPAGRQCCMESQDWSAFDQFVRTT
                     LRQHLHAGGVRLYELTAGGAWLEPLVRPAAGPPPRLAAGEGAVARAISSGQAVVVDEM
                     HVSPQDRDSNACQPTSASPGALPLQPGSEQRTPRWLLPLRTGTRTEVLVTVAGLDLPH
                     AHEEHLANAVRVQLQLFWRQISAARLLSRYLRIDRQTGMLERVELLNFLDELVGACNR
                     KGEPVMVLALALEGMRKLDDAGFWRQRDSLIQDLGRALRAKIRADDVLGRFSDDRFVV
                     VLRGLEPALATRVAEKLLDTVRAQAIQAACQLNHQGRAEPWQLKVRAGLAGIGPHCAP
                     CSRPFATFDPQAGAPPTPEAVHTGQSVLQRALGLIEYARRERVELVTDLMAGVPDSLA
                     RNPRGYPPQTQSPATVVNGTGQSPASDANQPMTMECSDDTACADPH"
     assembly_gap    3114..3213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3214..4370)
                     /locus_tag="PLU99_06855"
     CDS             complement(<3214..4370)
                     /locus_tag="PLU99_06855"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOO16812.1"
                     /translation="MSESLEISLGEPGEWLEKLELLRQENEALGRELLRCYEQLNFVF
                     EITEKIAKLQDPETILRLLLQRFGKTIGAGALFLDQGGRCLAIPPGDRYAWSINIDAE
                     VVQSCLSGQIEQARRGGHAVVPADAVELQDVLDGAHVLLVPLHQEPARSAVVIVLRAS
                     DEPAFDSGDMLASESVLGYGGQVLANVLMVRRLQQTAIETVRALANAIDAKDNYTCGH
                     SERVGWLARQVGIELDLPAAELEFLEWAGILHDVGKIGIPEAILNKPGRLTQEEFEQI
                     RRHPHMSYEVLRPVGRLGPVLSAVLYHHENWDGTGYPHRLSGEQIPLSARVIRVVDTF
                     DALTSTRAYRRGFPIAKALEILAKESGRGTDPQITPVFIRLMQRLERERPP"
     gene            complement(4388..5812)
                     /locus_tag="PLU99_06860"
     CDS             complement(4388..5812)
                     /locus_tag="PLU99_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16813.1"
                     /translation="MDRKNAEREPVTHPPAPRAETSALLWWGAAIALASGAALLLLRL
                     RTRQIRITRDVRPQSAAPQERVHQLLELLLERHAAASDSDNWSALLCAVEQLQRELEA
                     HCGSNPSGVALEARAARTGAQHILDALPVGILQLDASGELRYANAAARHLLQLRGGAA
                     GARLEHCLAQPALAETILNLRHHVGGVGVDCRFSTPDGPVVARLSAIAPAPGRDELVI
                     VAQDISELKQVERTRDEFLAHLTHELRTPLTNIQAYAETLKDDFFDDEKTRRECYDVI
                     MGEARRLAKLIEDVLSVSQIDAGAARLTRGPVRVDDLLRAAAREVQAAADAKGIELVL
                     EIPPRVPTLQGDRVRLQQVWANMLSNALKFTPSGGAIRAGIEPRGHVMRLSVADTGIG
                     IAPRWHKLIFEKFFRVQEPAVQATEGTGLGLTLVRDIVRLHGGSVQVDSTPGRGARFT
                     VELPLAAWTTERPVSEHSTGGDAI"
     gene            complement(6103..7032)
                     /locus_tag="PLU99_06865"
     CDS             complement(6103..7032)
                     /locus_tag="PLU99_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO16814.1"
                     /translation="MRVLVTGCAGFIGCHLSERLLREKHEVVGLDNLASGSRPNVDWL
                     AAFPTFQFVEHNLSQPLPPLGRFDLICNLACPASPVDFDRIPLEILAVCSAGMRNVLE
                     LACACNAPLLHTSTSEVYGDPKEHPQRETYWGHVNPIGKRSCYDEGKRFAEALAVAYR
                     DSRAARPRIARIFNTYGPRMRPDDGRMLPNFILQALAGKPLTVHGDGSQTRSFCYVSD
                     MVDGLLRLALSDVSEPVNLGNPVEITVLQAAEEVIRLTNSSSRITFVPRPPDDPCIRC
                     PDITRAQQRLGWSPQVDRQDGFSRTIEWFRTGR"
     gene            7175..8491
                     /locus_tag="PLU99_06870"
     CDS             7175..8491
                     /locus_tag="PLU99_06870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOO16815.1"
                     /translation="MRIGVIGSGYVGLVTSACLADSGNHVMAIDNDESKIAELSAGRC
                     PIFEPGLEELLGENLRAGRLRFSTDLAEGVRGARVVFIAVGTPPRPDGSADLSVVEFV
                     AAQIGRTIDSPTIVVIKSTVPVGTGARIEALIRGLCPHTCHVVSNPEFLKEGNAVNDF
                     LRPDRVVIGAEDEAAGDTVAELYRPFVRNNKPILRMSRAASELTKYAANAYLATRISF
                     INEIADICERYGVDVDEVRLGIGSDERIGYHFMYPGIGYGGSCFPKDVQALASTARSG
                     NVHCSLLEAVHERNLYQRTAMVRRITSRLGPDLRGRRIAVWGLAFKPKTDDIREAPAI
                     AIISALLEQGVNIVAYDPRAEGPAREVFRDQVAYDSDAYLALRGADALLICTEWNEFR
                     SPDFERMRREMKQPLIFDGRNLYEPATVARYHFEYHSVGRPPVRPL"
     gene            8649..9710
                     /gene="amrS"
                     /locus_tag="PLU99_06875"
     CDS             8649..9710
                     /gene="amrS"
                     /locus_tag="PLU99_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO16816.1"
                     /translation="MSATVKLTELLHQHTAPAAAELVRPEPEGALRCLACAHRCRVLP
                     GRPGVCRVRWNEGGTLRVPYGYVAGLGVDPIEKKPFYHAYPGATAMSFGMLGCDFHCS
                     FCQNWVTSQALRDDEAISSPVLIEPQRIVELALEHGCRVLTSTYNEPLITAEWAVEIF
                     KLGRPHGLVGSFVSNGNATPEVLEYLRPHVELYKIDLKAFRDETYRKLGGVLENVLNT
                     IRLARQMGFWVELVTLLVPGMNDSDEELKQMTAFIAGVSPQIPWHVTAFHPDYKMTQP
                     PRTTSEMLERAYDIGRQAGLQFIYAGNLPGQVGDRENTKCPACGQVLIERQGFSIRLN
                     RLRAGRCPACGTEIPGVWE"
     gene            complement(9729..12953)
                     /locus_tag="PLU99_06880"
     CDS             complement(9729..12953)
                     /locus_tag="PLU99_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOO16817.1"
                     /translation="MIAKILQFAVQQRLLMVLLAIGLAALGIWNFSRLPIDAVPDVTN
                     MQVQINTAVTGLAPVEIEKQITAPIEWAMQGIPGVEQIRSVSFYGVSQVTVIFHDNVD
                     IYRARQLVSERLAEAKESLPENVGTPFLGPIWTGLGEIYFWSVDAVGPKPDGTPYTDM
                     DLRTIQDWIIKPQLRSVPGLTEINSIGGYEKQYHVTPDPMKLIAYHLTFRDVLEALAA
                     NNANVGGGYIEHAGEQYTIRATGRIQSIEDISNVKLGTHDGVPIYVKDVAQVALGKEL
                     RTGTGTANGREAVVGTAILLYGENSRTVAHRVGQKLKEINKTLPENVVTTTLYDRSYL
                     VDATLRTVRENLTLGAMLVIAVLFLLLGNVRAAIIVALTIPLAMLFAISGMVQSKISA
                     NLMSLGAIDFGIIVDGTIVMVENIMRRLGLRQHELGRRLTREERHQLVQQAAAEVRRP
                     TLFGELIIMIVYLPIITLTGVEGKMFRPMALVVLLALAGATLLTFTVVPALCALLLGG
                     KVSEKEVFLVRWAKAIYRPIVEWAIRLRWGVSVAAVAVVTATLALATHVGSEFIPQLS
                     EGALALQPTRMPSIALTTSAQMQERVESELLKEFPDEIAAIFSRTGTSEIVTDVCGPE
                     ISDTYMLLKPRHQWKKAKTQEELAEAMEAVVHDLPGQNYEFSQPIELRMNELIAGVRS
                     DLAVKVYGDDLDTLMQYAAQIAEELRQVPGAADIKIEQLAGMPMITIDIDRAAVARYG
                     LNVQDVQEVVEIALGGVAAGQVLEGDRRFELVVRMPEELRGDIGGIGKLPIPLPRREP
                     ESYAALTSHNLQSLRGSRADVAQPIAPAYIPLSSLAKIDITEGARQIRREDGKRRAVV
                     QCNVRGRDLGGFVAEAQQRIEATLGKLPEGYWLGWGGQYENLVAARQRLLIVVPLALG
                     LIFILLFAAFNSLRQALLVFTGVPLALTGGVLALWLRGIPFSISAAVGFIALSGVAVL
                     NGLVMVGFINQLRRRGLGLHEAIVQGSLTRLRPVLMTALVASLGFVPMALATGTGAEV
                     QRPLATVVIGGIISSTLLTLVVLPAIYGLFAHHDSRHGVSEVTV"
     gene            complement(12999..>13245)
                     /locus_tag="PLU99_06885"
     CDS             complement(12999..>13245)
                     /locus_tag="PLU99_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HOO16818.1"
                     /translation="VLDRKSVPVLVPIEAVQRLKGESVVFVSDEHGFEPRSVAVGRTS
                     STHAEIVSGLAAGEPYVARGAMILKAEVGKRGAVHEH"
BASE COUNT         2310 a         4095 c         4358 g         2382 t
ORIGIN      
        1 gcgaccggca gaacttgccc ttcatgttct ggccgaaggt gtaatgccag atgatccagt
       61 ttcgcaggtg caggccgagt tgccggccga cgatgcgcac gtcggcggcg aactcgtcgc
      121 cgatggcgat gaagaatgaa ccggtcggct tgagcacgcg ctggcaggcg gccatccagc
      181 gtttggtcca ggcgacgtat tcggcgtttg gtaagtcgtc gtggtatttg tcgtagacgt
      241 agcctatgtt gaagggcggg tcggcgaaga ccaagtcaac gcagccgggt tcccagcttt
      301 cgagcagttg gcacgaatcc gcacaatata ttgtgttcag ctccatggtt caccgctaca
      361 aatgggccga actcattttg acggaaaagg gtaggcagcg tcaactctaa ccgtttgaaa
      421 accgctctca cttctgcgga gttgtttcgg gagcgctccc ggaagcgcgc atattcacga
      481 tttccgcgcg cagggccccg atccgtcgcc agtgcgtgcc ctcccagtag acctgtcggc
      541 actcccggca gcggtaaaac tcacgcaccc agatcaggct gcgggccggc acgacgtcgg
      601 ccaccgccgc ccggtccacc ggctccagtt cgccgttgca gacggtgcag cgcggaaatc
      661 cgaccggtag cttcagccgc ccggccacga accgcacctg gtccgccagg cgcagaccga
      721 ccgggatgaa cagcccgggc agctcgccgg tagtgaacac gcggcgttcg aagagcttgc
      781 ggtcggaaga taggacgacg cgccgctcgg ccaaggcctg ccgcacgagg gctgagtcct
      841 cgatatcgcg gacgtaggcg gtgttgacgc ccagcacgcg cagccaacgt gccaggccgc
      901 cgcacatcgc atcgcaggcc acgcgcaggg cttcgttcgc atcgcaggcc acggccgaag
      961 catcacaggg caagcgcaga gcgtccttgt cgttttcagc gttcacaaac ttattgtaag
     1021 cggagggagg cggtgcggac cggcattcgc gcggcgcatt tgcggcttcc gtcggtgaat
     1081 gaaaaaccat ccaccacgat cccgaagtcg ttcaggttct gccatgtccc gtcctgaatc
     1141 ttcagtagcg gtcgtaacag acgttgttcg cgtcccgggc gtttgaattg acggcaatgg
     1201 cgcccgactg gcaattgtat acccagcccg cgtcggcggc ccattcctga aaggccggcg
     1261 gatcggtggt catgactacg cccgactttc ctaaccgcgg cagcacgggg caggccggta
     1321 tgccgtctcg cagatagggc ccgaagcacg cttgattgct cttctcgggc gacacgcggc
     1381 cctcggcgtc gctgaaggag gtgagctgcg ccacgaacgc ttcggcactg ccgggtgggt
     1441 gaatgccgtc cgagtgctgc ccgggataaa ctccgtggtc gtaatagtac atttcaatgg
     1501 cgttgcgcaa cgtcaccagt gcgggacgag gattgagcgt ctgcggtccc ggctcggcct
     1561 ggctgaaacg cggcactgcc agaaccgcca gcaagccaat gatcatgatc acgattatgg
     1621 cttcaatgag ggtcagcgca ggccgtgtca tcggaacact ccatcgtcat aggctggttt
     1681 gcgtcgcttg cggggctctg gccggtgccg ttcacgaccg tggcggggct ctgtgtctga
     1741 ggcgggtaac ctctagggtt tcgcgccagc gaatccggca cgccggccat cagatcggta
     1801 accagttcga cgcgctcccg gcgggcgtac tcaatcaggc cgagggcgcg ctgcagcacg
     1861 ctttgaccgg tgtgtaccgc ctcaggcgtg ggcggcgcgc ccgcctgggg gtcgaaggtg
     1921 gcgaaggggc gcgagcaggg cgcgcagtgc ggtcctattc cggccaggcc ggcgcgaact
     1981 ttcaactgcc agggctcggc acggccctgg tgatttagct gacacgcggc ctggatggcc
     2041 tgagcacgga cggtgtctag cagcttttca gcaacccgcg tggccagggc aggctcgagt
     2101 ccgcgcagca cgacaacgaa gcggtcgtcg ctgaaccgcc cgaggacgtc gtcggcgcga
     2161 attttggcgc gcagcgcccg ccccaagtcc tgtatgaggg agtcacgctg ccgccaaaaa
     2221 cccgcgtcgt ccagcttgcg cattccttcc aacgcaagtg caagcaccat gaccggttcg
     2281 cccttgcggt tgcacgcgcc caccagttcg tccagaaaat tcagcagctc gacgcgctcg
     2341 agcatccccg tctgccggtc aattcgcagg tagcgcgaaa gcaagcgcgc agccgaaatt
     2401 tggcgccaga agagctgaag ctggacgcgc actgcgttgg ccaagtgctc ttcgtgtgcg
     2461 tgcggcagat ccaggccggc cacggtgacc aggacttcag tccgcgtgcc ggtccgtaag
     2521 ggcagcagcc agcgcggcgt gcgctgctca gaaccaggct ggagcggcag ggcgccaggc
     2581 gaagcggagg tcggctggca agcgttagag tctctgtcct gcggcgacac atgcatttcg
     2641 tcgacgacga cggcctgccc ggagctgatc gcgcgggcga cggccccctc accggccgcc
     2701 agtcgcgggg gcggaccggc tgcgggacgc accagcggct ccagccaggc gccgccggcc
     2761 gtcagttcat ataggcgcac accgcccgcg tgcaggtgct ggcgcagggt ggtccgcacg
     2821 aactgatcga aagcggacca gtcttgcgac tccatgcagc actgccggcc ggcgggggag
     2881 tcgacccagg cgcagaagtc gttggccatg ttggcggacg cgtacatgaa tcgccggcgc
     2941 tgcgccgtac cgcctggcgg caccgccgcg gctgcatggc tcgcgctggc ggagatggcg
     3001 ccgcgcggat tgctttggca gcgctgttgt ttaccggctg cgccggccac cccccccagc
     3061 aggcaaagaa tcaacgagga taagccaaca aaggctgcca ggcttgaacc ggtnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccggcg ggcgttcacg ctcgaggcgc
     3241 tgcatcagcc gaatgaagac cggcgtgatc tgcgggtcgg tgccgcggcc cgactctttc
     3301 gcgaggattt ccagggcctt cgcaatgggg aagccgcgcc ggtaagcgcg cgtggaagtc
     3361 agcgcgtcga atgtgtccac cacgcgaatt acccgcgccg agagcggaat ctgctcgccg
     3421 ctcaagcgat gcggataccc cgtcccgtcc caattctcat ggtgatagag gacggccgag
     3481 agcaccggcc caagccggcc caccgggcgc agcacttcgt agctcatgtg cggatggcgg
     3541 cgaatctgct cgaactcctc ttgcgtcagc cgcccgggtt tgttgaggat cgcctcggga
     3601 atgccaatct tgcccacgtc gtgcaggatg ccggcccact ccaggaactc cagttcggcg
     3661 gccggtaaat ccagttcaat cccgacttgc cgcgccaacc agccaacccg ctccgaatgg
     3721 ccgcaggtgt agttgtcttt tgcgtcgatg gcattggcca gggcgcgcac cgtctcgata
     3781 gcggtttgct gcaagcgccg caccatcagc acgttcgcca gcacctgccc gccgtagccc
     3841 aagaccgatt cggaggccag catgtcgccg gaatcaaagg cgggctcgtc gctggcacgc
     3901 aaaacaatta cgaccgcgga acgtgccggc tcctggtgaa gtgggaccag cagtacatgc
     3961 gcgccgtcga gtacgtcttg cagctccacg gcgtctgccg gaaccacggc gtgtccgcca
     4021 cggcgggcct gctcgatttg gccgctcagg cagctctgaa caacctcggc gtctatgttt
     4081 atgctccagg cataccggtc gcccggcgga atcgccaggc agcggccgcc ttgatcgagg
     4141 aacagcgccc cggccccaat ggtcttgccg aagcgttgca gcagcaggcg cagaatggtt
     4201 tcggggtcct ggagcttggc gattttctca gtaatttcga agacgaaatt gagctgttcg
     4261 tagcagcgca gcagctcgcg ccccagagct tcgttctcct ggcgcagcag ttccagtttc
     4321 tccagccact cgccgggttc gcccaggcta atttccaacg attcgctcaa tgcctgctca
     4381 gagccgctca tatcgcatcg cccccggttg agtgttcact cacgggcctt tccgtcgtcc
     4441 aagcagccag cggcagctca accgtgaacc gggcgccgcg gccgggcgtg ctgtccactt
     4501 gtaccgaccc gccgtgaagc cgcacgatgt ctcgtaccag cgtcagcccc agcccggtcc
     4561 cctcagtggc ttgcaccgcc ggttcctgca cgcggaagaa cttctcgaat atcagcttgt
     4621 gccaccgcgg agcgatgccg atgccggtgt ccgccacgct cagtcgcatg acgtgccccc
     4681 ggggttcgat gccggctctg atcgccccgc cggagggcgt gaatttgagc gcgttactca
     4741 gcatgttggc ccacacctgc tgcagccgca cgcgatctcc ctgcagcgtg ggcacgcgcg
     4801 gcggaatctc caataccagt tcgatgccct ttgcgtctgc ggcggcttgc acctcacggg
     4861 cggcggcacg cagcagatcg tcaacgcgca ccggtccgcg ggtgaggcgg gccgcgcccg
     4921 cgtcgatctg cgagacgctc agtacatcct cgatcaactt ggccaggcgg cgcgcctcgc
     4981 ccatgatgac gtcgtaacat tcgcggcgcg tcttttcgtc atcgaagaag tcgtccttaa
     5041 gtgtttcggc gtaggcttgg atattcgtca acggcgtccg cagctcatgc gtcagatgcg
     5101 ccaggaactc gtcgcgcgtg cgctccacct gcttgagctc cgaaatgtcc tgcgcgacga
     5161 tcaccaactc atcgcgaccg ggtgcgggtg cgatggcgga caggcgcgcc acgaccggcc
     5221 cgtccggcgt gctgaagcgg caatccacgc ccacgccgcc cacgtggtgc cgcaggttga
     5281 ggatggtctc agccagggcc ggctgggcca ggcagtgctc caagcgcgcc ccggccgcgc
     5341 cgccgcgaag ctggagcaga tgacgggcgg ccgcatttgc atagcgcagc tcgccgcttg
     5401 cgtctaactg cagtatgccg actggcaaag cgtccagaat gtgttgcgct ccggtgcgtg
     5461 ccgcgcgcgc ttctaaggcc acgccgctcg ggttgctgcc acagtgcgcc tcgagttcgc
     5521 gctgcaactg ctcgactgca cagagcagtg cgctccagtt gtcgctgtca ctggcggcgg
     5581 cgtgccgctc cagcaggagc tccagcagtt gatgaacgcg ctcctggggc gccgctgatt
     5641 gagggcggac atcgcgggtg atccgtattt ggcgggtgcg gagtctgagc agcaacaggg
     5701 ccgcgccgct ggccagggcg atggccgcgc cccaccacaa tagtgcgctg gtttcggccc
     5761 gcggcgccgg cgggtgagtg accggttcac gctcggcgtt ttttctgtcc acgctgtgaa
     5821 ccagcgttgt gattactgca ccggacagga tgatggccag agacagcagc aggcacgggc
     5881 ggcttggccg gcgcgtccga taagtgctgt cgccggacgc gcgaccgcca gcgagtgccg
     5941 tgcgcagcct caacggaagc aagggcgatc cctggccggt gccgttcata ctgcgtttat
     6001 cgagcagggg gaaacgctgc ttcgcacgcc tcagcgggca cgcaggcgcg agccggcatg
     6061 cttaagtatt tgggacggtt ccgaaagcgc ggtcttatat tgttagcgcc ctgtgcggaa
     6121 ccattcgatc gtgcggctga acccgtcctg acggtcgacc tgtggtgacc agcccaggcg
     6181 ctgctgggcg cgggtgatgt ccgggcagcg aatacaagga tcatccgggg ggcgcggcac
     6241 gaaggtgatg cggctggaac tattggtcag gcgaatcacc tcctcggccg cctgaagaac
     6301 ggtgatttcg accgggtttc cgagattgac cggttcggaa acgtcgctca gcgccaagcg
     6361 cagcaggcca tcgaccatat ctgagacgta gcagaaacta cgggtctggc tgccgtcgcc
     6421 atgcacggtg agcggcttgc cggccagggc ctggaggatg aagttcggca gcatgcggcc
     6481 gtcgtcgggg cgcatgcgtg gcccgtaagt attgaaaatg cgtgcgatgc gggggcgcgc
     6541 cgcgcgggaa tcgcgatagg cgacggccag cgcttccgcg aagcgtttgc cttcgtcgta
     6601 acaggagcgc ttgccgatcg ggttcacgtg cccccagtag gtttcgcgct gcgggtgctc
     6661 tttggggtca ccgtagacct cgctggtgct tgtgtgcagc agcggcgcgt tgcaggcgca
     6721 ggccagttcg agcacgtttc gcatcccggc cgagcagacc gccagaattt ccagcggaat
     6781 gcgatcgaaa tcgaccgggg aagccggaca cgcgaggttg cagatcaggt cgaaacgccc
     6841 gagcggtggc agcggttgcg acaggttgtg ctcgacgaat tgaaatgtcg gaaaggccgc
     6901 cagccagtca acgttagggc ggctgccgct ggcgagattg tcgagcccga caacctcgtg
     6961 tttctcccgc agaagccgtt cggacagatg gcatccgata aagccggcgc agcctgtgac
     7021 caaaactcgc atttagcagc ctccaggagc gcgggggcgg aataaagtca ttggtgcccc
     7081 gcgcaccaag cgtttcgatc cattatactg gcgaggtttc ggcggtacga gcgcgctcac
     7141 aagcgagcgg gcgggcagcc gtagagggaa ctgtatgcga atcggcgtca tcggcagtgg
     7201 ttatgtgggg ttggttacga gtgcgtgcct ggccgatagc ggcaatcacg tgatggcaat
     7261 cgataacgat gagagcaaga ttgccgagtt gtctgccggg cggtgcccga ttttcgagcc
     7321 cgggctcgaa gaactgctgg gcgaaaatct gcgggccggc cgtctgcggt tctcgaccga
     7381 tctcgcggag ggggtgcgcg gggcgcgggt cgttttcatc gcggtgggca cgccgccgcg
     7441 gccggacggg tcggccgacc tgtcggtggt ggagttcgtg gcggcgcaga tcggtcgcac
     7501 gatcgactca cctacgatcg tggtgattaa gagcaccgtg ccggtgggca ccggggcgcg
     7561 cattgaggca ttgattcgcg gactgtgccc gcacacctgc catgttgtca gcaaccccga
     7621 gttcctgaag gaaggcaatg cggtcaacga cttcctgcgt ccggaccggg ttgtgatcgg
     7681 cgctgaagat gaggcggcgg gagatacggt tgccgagctg taccgtccct tcgtgcgcaa
     7741 caacaagccc atcctgcgaa tgagccgcgc cgccagcgag ctgaccaagt acgcggccaa
     7801 tgcctacctg gccacgcgta tcagcttcat caacgagatt gccgacatct gcgagcgcta
     7861 cggcgtggat gtcgacgagg tgcgccttgg gatcggctcc gacgagcgga tcgggtacca
     7921 cttcatgtat cccggcatcg gttatggcgg aagctgtttc ccgaaggacg tccaggcctt
     7981 ggccagcacc gcacggtcgg gcaatgtcca ctgcagcctg ctcgaagcgg tgcacgagcg
     8041 caacctgtac cagcggacgg ccatggtgcg acggatcacc agccgtctgg ggcccgactt
     8101 gcgtgggcgg cgtatcgcag tgtggggctt ggctttcaag ccgaagaccg atgacatccg
     8161 cgaggcgccg gccatcgcga ttatttcggc gctgctcgaa cagggcgtga acatcgtggc
     8221 ctatgaccca cgcgccgagg gaccggcgcg cgaagtcttc cgcgaccagg tggcatacga
     8281 cagcgatgcc tacctggcgt tgcgcggcgc cgacgccctg ctgatctgca ccgaatggaa
     8341 cgagtttcgc agcccggatt tcgaacgcat gcggcgcgag atgaagcagc ccctgatctt
     8401 cgacggccgc aatttgtatg agccggccac ggtggcgcgc tatcattttg agtaccacag
     8461 cgttgggcgc ccgccggttc ggccgctcta gcggcagtca tcagttcagt agggcggggc
     8521 gtcgggtgcg atgccgaagc gctgggcgcc atgtccaagc gcggggtgcc atgcccggcg
     8581 tgggtgccat gcccaagcgc taaagcgcgg gcatgccgcg tttgattgaa gagaatctag
     8641 agaattcgat gtctgccacc gtcaaactga ccgaactcct gcaccagcac accgccccgg
     8701 ccgccgcgga actcgtgcgc ccggaacccg agggcgcgct gcgctgcctg gcctgcgccc
     8761 atcgctgccg cgtattgccc ggccgcccgg gcgtgtgccg cgtgcgctgg aacgagggcg
     8821 gcactctccg cgtgccatat ggctatgtcg cggggctggg agtcgacccg atcgagaaga
     8881 agccctttta ccacgcctat cccggcgcga cggccatgtc attcggcatg ctgggctgcg
     8941 acttccactg cagcttctgt cagaactggg tcaccagcca ggccctgcgc gacgacgaag
     9001 ccatctcgtc cccggtgctg atcgagccgc agcggatcgt cgagctggcg cttgagcacg
     9061 gctgccgcgt gttgaccagc acctacaacg agccgctgat caccgcggaa tgggccgtcg
     9121 agattttcaa gctgggccgg ccgcatgggc tagtgggctc gttcgtttcc aacggcaacg
     9181 ccacgcccga agtattggaa tacctgcgcc cgcatgttga gctgtacaag atcgacctca
     9241 aggcgtttcg cgacgaaacc taccgcaagc tgggcggcgt gctggaaaac gtcctgaaca
     9301 cgatccggct ggcgcggcag atggggtttt gggtcgagtt ggtcacgctg ttggttccgg
     9361 gcatgaacga ttcggatgaa gagctgaagc agatgacggc tttcatcgcc ggcgtctcgc
     9421 cgcaaattcc ctggcacgta acggcgtttc acccggacta caagatgacg cagccgccgc
     9481 gcaccaccag cgagatgctg gagcgggcct atgacattgg gcggcaggcc gggctgcaat
     9541 ttatttacgc cgggaacttg ccgggtcagg ttggggaccg cgagaacacc aaatgtcctg
     9601 cgtgcgggca ggtgctgatc gagcggcagg gtttttctat acgcctgaat cgcctgcgcg
     9661 cgggacgctg tccggcgtgc ggtacggaga ttccgggcgt ttgggaatag ctgcccgtcc
     9721 cacgagcttc atacggtcac ctctgataca ccatgccgag aatcgtggtg cgcaaaaagc
     9781 ccgtagatgg cgggcaggac tacgagcgtc agcagcgtgc tgctgatgat gccgccgatc
     9841 actaccgtcg ccagtgggcg ctgcacttcg gcgccggtgc cggtggccag ggccatgggg
     9901 acgaaaccca gcgaggcgac cagcgcggtc atcagcaccg ggcgcagacg cgtcaggctg
     9961 ccctggacga tcgcttcatg cagacccagc ccgcgccggc gcagttggtt aatgaagcct
    10021 accatcacca ggccgttgag cactgccacg ccggagaggg cgatgaagcc cacggccgcc
    10081 gagatagaga aggggatgcc gcgcaaccac agggcgagca cgccgccggt gagggccagc
    10141 gggacgccgg taaacacaag caaggcctgt cgcagagaat tgaaggccgc aaagagcagg
    10201 atgaagatca ggcccagggc cagcgggacc acaatcagta accgctggcg cgccgcgacc
    10261 aggttctcgt attggccgcc ccacccgagc cagtagccct ccggcaactt gccaagcgta
    10321 gcttcgatgc gctgctgggc ctcggcgacg aatccgccca ggtcgcggcc gcgcacgttg
    10381 cactgtacga ccgcacggcg cttgccgtct tcgcggcgaa tctggcgggc gccttcggta
    10441 atgtcgattt tggccaggct agaaagcgga atgtaggccg gcgcgatcgg ctgcgcgacg
    10501 tcggcgcgcg agccgcgcag gctctgaaga ttgtggctgg tcagagcggc gtagctctcc
    10561 ggctcgcgcc gcggcagggg gatcggcagc ttaccgatgc caccgatgtc gccgcgcagc
    10621 tcttcaggca tgcgcaccac aagttcgaag cgccgatcac cttcaagcac ctgccccgcg
    10681 gcgacgccgc ccagcgcgat ctccactact tcctggacgt cttgaacgtt caagccgtag
    10741 cgggcaacgg ccgcgcggtc gatatcaatg gtgatcatgg gcatgccggc cagttgctcg
    10801 attttgatgt ccgccgcgcc gggcacctgg cgcagttctt ctgcgatttg ggcggcgtac
    10861 tgcatgagtg tgtccaggtc gtcgccgtag actttgacgg ccaggtcgct gcggacgccg
    10921 gcgatgagtt cgttcatgcg cagctcaatg ggttggctga actcgtaatt ctggccgggt
    10981 aggtcgtgga ctacggcttc catggcttcg gccagttctt cttgcgtttt tgctttcttc
    11041 cactggtggc gcggcttgag gagcatgtag gtgtctgaga tttccgggcc gcagacgtcg
    11101 gtgacgattt cggaagtgcc ggtgcgtgag aagatggcag cgatctcgtc tgggaattct
    11161 ttcagcagtt cggactcgac tctttcctgc atctgggcgc tggtggtcag cgcgatgctg
    11221 ggcatgcggg tcggttgcag cgccaaggcc ccttcgctga gctggggtat gaactcgctg
    11281 ccgacgtgcg tagccaaggc gagcgtggcc gtcaccacgg cgaccgcggc gaccgatacg
    11341 ccccagcgca ggcgaatagc ccattccacg atggggcgat agatggcctt ggcccagcgg
    11401 accaggaaga cttctttttc actcaccttg ccgccaagca gcagggcgca cagcgccggc
    11461 acgacggtaa aggtcaacag cgttgcaccg gcgagggcca gcaggacgac cagtgccatg
    11521 gggcggaaca ttttgccttc gacgccggtc agggtgatga tcggcaggta gacaatcatg
    11581 atgatgagct cgccgaatag cgtgggccgg cggacctcgg cggcggcctg ctgcaccagt
    11641 tgatggcgtt cttcgcgggt gaggcggcgg ccaagttcgt gctgccgcaa ccccaagcgg
    11701 cgcatgatgt tctcgaccat gacgatggtg ccgtcgacaa tgatgccgaa gtcgattgcg
    11761 cccaagctca tgaggttagc gctgattttg ctttgcacca tgccgctgat cgcgaagagc
    11821 atggcgagcg ggattgtcag ggcgacgatg atcgcggcgc ggacgttgcc cagcagcagg
    11881 aaaagcacgg cgataaccag cattgccccg agcgtcaaat tctcgcggac ggttcgcagc
    11941 gtggcgtcga ccaggtagct gcggtcataa agagtggtag tcaccacgtt ctcgggcagt
    12001 gttttgttga tctctttcag cttctgcccg acgcggtgcg caaccgtgcg gctgttctcg
    12061 ccgtagagca ggatggctgt gccgaccacc gcttcgcggc cgttcgccgt tccggtgccg
    12121 gtgcgcagct ctttgcccag tgcgacctgg gcgacgtcct tgacgtatat gggcacgccg
    12181 tcgtgcgtgc cgagcttgac gttggagata tcctcgatgc tctggatgcg gccggtcgcg
    12241 cggatggtat attgctcgcc cgcatgttcg atatatccgc cgccgacatt ggcgttgttg
    12301 gccgccaggg cttccagcac gtcgcgaaat gtgaggtgat aggcgatgag cttcatcggg
    12361 tcgggggtga cgtgatactg cttctcataa ccgccgatgc tgttgatctc cgtaaggccg
    12421 ggcacggagc gcagttgcgg tttgatgatc cagtcctgaa tggtgcgcag gtccatgtcc
    12481 gtgtaaggcg tgccgtccgg cttagggccg acggcgtcaa cgctccagaa gtagatttcg
    12541 cccaggccgg tccagatcgg gccgaggaac ggcgtaccta cattctccgg cagactctcc
    12601 ttggcttcgg ccagtcgttc gctgacgagc tggcgggcgc ggtagatgtc cacattgtcg
    12661 tggaagatca ctgtcacctg cgagacgcca taaaaagaca ctgagcgaat ctgctccacg
    12721 ccgggaatgc cctgcatggc ccattcaatg ggggcggtta tctgtttctc gatctccacc
    12781 ggagccaggc cggtgaccgc ggtgttgatt tgcacctgca tgttggtgac gtccgggacg
    12841 gcgtcgatcg gcaggcggct gaagttccaa atgcccagcg cggccaaacc gatggccagg
    12901 aggaccatca gcaagcgctg ctgcaccgcg aattggagga tttttgcgat cattccaaat
    12961 atccggtcaa gtgttcacag ttgacggttt atgcggcatc agtgttcgtg gactgcgccg
    13021 cgcttgccca cttccgcctt aagaatcatc gccccccgcg ccacgtaagg ctcgcccgcg
    13081 gccaggccgc tcacgatttc cgcatgtgtg ctgctcgtgc gaccgaccgc gacggagcgc
    13141 ggctcgaagc cgtgctcgtc gctgacgaag accacggatt cgcccttgag acgctgcact
    13201 gcttcgatgg gtacgagcac cggcacggat ttgcggtcaa gtacg
//
LOCUS       DAOSYS010000083        13092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_3994_length_13092_cov_5.581882, whole genome shotgun sequence.
ACCESSION   DAOSYS010000083 DAOSYS010000000
VERSION     DAOSYS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3994_length_13092_cov_5.581882"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..833)
                     /locus_tag="PLU99_06890"
     CDS             complement(90..833)
                     /locus_tag="PLU99_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOO16819.1"
                     /translation="MPEPLLTRYLQPLITGRRAECFALIADALAAGQAAEELICDVVW
                     PAMVQVDRLYRGDRINSAAEHMACRINRAVVGQLQPHLAQSPRNQKRVLVTCGVGDQE
                     ELGAGIVADLLQADGWEVFHVGGGVPEDEILTMVGQVRPQVLVLFATRPEDVPDTRRL
                     VEMIREVGVCPTMNIVVSGGIYNRADGLWQEVGADLFSPSPRELRAALRDLQPRQPHA
                     PRVAVVKKRRRRRKVAGAGTPEPACAGQQ"
     gene            complement(1074..2204)
                     /locus_tag="PLU99_06895"
     CDS             complement(1074..2204)
                     /locus_tag="PLU99_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOO16820.1"
                     /translation="MFWMYAGFVALVLLLLTLDLGVFHRKAHVISIKEALGWSAFWIA
                     LGLAFSVFIYFGYENHWLGLGLTWDMMSHVPQAPEDGLTVYNDGYSATVKYITGYLVE
                     KSLAVDNIFVIAMLFTYFGVPAMYQHRVLFWGIVGALLMRGAMIAIGVQLIYRFAWII
                     YVFGAFLIITGARMLFSKPAAADPGHNVVVRATRRLLPITQHFHGQHFFVRAGSAAGR
                     EPVQPGSAVEPDSVVEAARPGALLATPLLLTLIMVEFTDLIFAVDSIPAIFAITADPF
                     LVFTSNVFAILGLRSLYFALAGIIDRFEYLKVALAVVLVIVGVKMLAHTWLKDLLGRH
                     FNLYLLAVVLAVLLAGVVASLIKKSGRNRSKATAEPSGNAGG"
     gene            complement(2376..3272)
                     /gene="metF"
                     /locus_tag="PLU99_06900"
     CDS             complement(2376..3272)
                     /gene="metF"
                     /locus_tag="PLU99_06900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOO16821.1"
                     /translation="MRIRELLSYGRPSISFEFFPPKDEAGFDRLRQTLASLRDLRPTY
                     VSVTYGASGAARRETIRLTTELRRDYGLEAMAHLTCVGSSQDELRSLLENIERGGVEN
                     LLALRGDPPTGTGPFQPHPQGFRYACELVALARKHSKFCVLGACYPEGHVEAPSLQAD
                     LDNLKRKVDAGCEVLITQLFFDNNKYFDFVARARAAGIGVPIIPGIMPITNVAQVKRF
                     TRTCGASIPATLVNELHRLQDDPHAVLSMGVAHATAQCIELLQRGAPGLHFYTLNKSP
                     ATRTILTAIRTVYPPANSPAGT"
     gene            complement(4110..4493)
                     /locus_tag="PLU99_06905"
     CDS             complement(4110..4493)
                     /locus_tag="PLU99_06905"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HOO16822.1"
                     /translation="MSAFACDVFLSYSSKDREAVRAIVERLRSDGLKVWFDEWVIKPG
                     DNIPAKIEEGIENSRVLVLCMSANAFGSDWARLESQTFRFRDPLNKDRRFVPVRLDDA
                     EIRGGSKRGRHQLRPRVLSFNNSPP"
     assembly_gap    4919..5018
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5326..6120
                     /locus_tag="PLU99_06910"
     CDS             5326..6120
                     /locus_tag="PLU99_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOO16823.1"
                     /translation="MKILVVLKQVPDSTTNIKIRPDGADIERAGVKMVVNPFDEFAIE
                     QAVLLKEKRSDVQSVTALIVGPAAAAEALRTALAVGADDGIHLQDPAFDQLDELQTAA
                     LVAAVVKPRGYDLILTGKQEIDLDSGQFGPALAEALDIPHVGACTKLEMAADGKSFTA
                     NRRIEGAEEVVEVPLPALITAEKGLCEMRYPSLPNLMKAKKKPVETLKAADVPGFNDL
                     VSAAGGMKLHTFRPPPERPPGKVLKGEPEQVVKDLVKLLREEAKAL"
     gene            6195..7178
                     /locus_tag="PLU99_06915"
     CDS             6195..7178
                     /locus_tag="PLU99_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOO16824.1"
                     /translation="MTNDILIFAEQRNDKIHPAALQIVTCGRALAAKTGGKVVACVIG
                     DATGGAADAMDKAGVDGLVIVNDPALAQYSALRYRTALSAVMQKVDPRVVLMPATFMG
                     RDLAPRVAMQRKAGLAVDAIDVQIGADGALDVTRPLYNGKATAHVAFAKDRPAIVSVR
                     ANTFPAPSSGAGGAARESVPYQAAAGDERITVKEVLKAGGEVKDVTEADIIVAGGRAL
                     KNQDNFQMLYDLAKALDAAVGASRAACDAGYQPHSRQVGLTGKTVTPKLYMAFGISGA
                     IQHLAGMRGSKTIVAVNTDPEAPLFKVADYSVVGDLFKIIPLLTEEVKKLH"
     gene            7383..7943
                     /locus_tag="PLU99_06920"
     CDS             7383..7943
                     /locus_tag="PLU99_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOO16825.1"
                     /translation="MPQKLDGLKVAILVTDGFEQIEMTSPRDALKQAGAQPVLVSPNH
                     DRVQGWDHQDKADIFPVDLPLDRADPNDFDALLLPGGVASPDKLRMNPKAVQFVRAFF
                     DQHKPVAAICHGSWTLIDADAVRGRRMTSWPSLRTDLRNAGATWVDEEVVVDGNLITS
                     RAPRDLPAFNRKVIEEFAAALAGARS"
     gene            complement(8037..10076)
                     /gene="ftsH"
                     /locus_tag="PLU99_06925"
     CDS             complement(8037..10076)
                     /gene="ftsH"
                     /locus_tag="PLU99_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOO16826.1"
                     /translation="MSEDLKPNQESRPNGGPPRPPRLTRGAFSWVLLVMLVITLLVMF
                     NRGMAPGQKIPISEFWTQVRNGNVKEVTIREDSIIGEFERLPSHAQGDSKRFECTYLK
                     AADKVEEIRKTIESDSPRPVTIKSEPSNSLLMNLLVTTLPWLVLFGVVWFLLFRQLRQ
                     TGGGPGMLGNFGRSRHRMTTKEHTNVTFADVAGIEEAKEEVYEVVEFLKNPKRFQRLG
                     GRIPRGVLLIGEPGCGKTLLAKAIAGEADVPFFSISGSDFVEMFVGVGASRVRDLFRQ
                     AKENSPCIVFLDEIDAVGRRRGLGFNGGGHDEREQTLNAILVEMDGFESNDQVIVIAA
                     TNRADVLDPALVRPGRFDRQVYVPLPDVKGRFEILRVHARKVKMQTPVDTKLQRLARA
                     TPMFSGADLAAIINESALLATLAGKDAIDMDDLEEARDKVRWGRAKRSRVVEEKDREM
                     TAYHEAGHAVVQTLLKDADPVHKVSIIPRGPYGGATFSLPEKDRQNYNRSHVEATLRV
                     LCAGRLAEELVGHDTNSGAAADIRQATMMARHMVTEWGMSDRVGFVYCGDEPLSTRTA
                     PDFSLGGKDLSPRTAEIIDEETKRIIDEAYADTRRILAENRDKLEAVARALLKYETLD
                     GEEVRALVNGESLNRPTVADLISAEQNRRLEAPLARPVTHRPQAGEEPGPIPTPA"
     gene            complement(10120..10623)
                     /gene="lptE"
                     /locus_tag="PLU99_06930"
     CDS             complement(10120..10623)
                     /gene="lptE"
                     /locus_tag="PLU99_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOO16827.1"
                     /translation="MKGQQFCLIVLVTLGLGGTVGCGYRHGGIHRCDVQTVYVDMFGS
                     KEFRRDLEFKLTEAIKKRINLDTPYRLAPPEKADTILKGELLEERQAAFAQDYASRQP
                     REMVLVLAARVQWKDLRSGRVLIDQPVELQAADYVPPEGQPEALAQERAINLLAARIV
                     AKMYEEW"
     gene            complement(10620..11678)
                     /gene="bamD"
                     /locus_tag="PLU99_06935"
     CDS             complement(10620..11678)
                     /gene="bamD"
                     /locus_tag="PLU99_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOO16828.1"
                     /translation="MALAVAAPAGETASIAPDLANQAGVAQNDQSQDQPSLPDMAGQP
                     EAPKAGEDQHYREREVLDPEIGEWVTEELAEDGASDELASARALLAEGQPKKARKILQ
                     QWIKENEGHERYYEAVFLLGETWFESKDYYRAYEQYELVVENTGGELFHTALFRERDV
                     ALAFLSGEKRVVWGIFRFPATDEAIDILDRIWERVPGTRLGEEALRTKADYRFSQGEM
                     DLAQDEYANLARQFPTGKYTQFAMMRSAVAAEAAFPGIKFDDRPLLEADERYRQVQAA
                     YPIYAERENVAQRLEGIRQQRAAKDLDIGKWYERTRQKSAAEYYYRLVLSDWGDTLAA
                     AEARQRLRTLGVEVEDAE"
     gene            12164..12862
                     /locus_tag="PLU99_06940"
     CDS             12164..12862
                     /locus_tag="PLU99_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOO16829.1"
                     /translation="MTAPFYDNESDSPAQRIVNLLARRGPSTVTQLVAELGVTTTAVR
                     QQVNRLVARRWLIRTRRRQGPGRPADVFALSDDARRMLAGQAEELSRLLLEQIAEMPG
                     RDRRRALLQAVSNRMAQNAQQFVGDGPPGERLRRLADHLSREGVLAEAETGRGAQRLT
                     VYTCPFYGVAAAHREVCDMERAAFSTLLGDALALERCVLDGHEHCEFRPRGAARGAAR
                     GKDAGNRAGKESLE"
     gene            12859..>13092
                     /locus_tag="PLU99_06945"
     CDS             12859..>13092
                     /locus_tag="PLU99_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOO16830.1"
                     /translation="MTKIADPLQTWAQSEYKYGFVTDIETETVPRGLSEEVIRLISAK
                     KHEPEWLTQWRLKAYRHWLTMTEPTWQNVHYAPI"
BASE COUNT         2372 a         4188 c         4078 g         2354 t
ORIGIN      
        1 gggcttgggc atggcgcccc gtttttcggg ctgggattta tataacccgc gtttcgcccc
       61 gtggcgcgcc gtcggacgcc cgcgcctgcc tactgctggc ctgcgcaagc cggctccggc
      121 gtgcccgcgc ccgcaacctt gcggcgccgc cgccgcttct tgacgactgc gacgcgcggt
      181 gcgtgcggct ggcgcggctg caaatcacgc agcgcggcgc gcagctcgcg cggcgaaggc
      241 gagaagagat cggcccccac ttcctgccac aggccgtcgg cgcgattgta gatgccgccg
      301 ctgacgacga tgttcatggt cgggcacacg ccgacttcgc gaatcatttc caccaggcgg
      361 cgcgtatccg gcacgtcttc cgggcgggtc gcgaacagca ccagaacctg aggccggact
      421 tgcccgacca tcgtgagaat ctcatcttcc ggcacgccgc cgccgacgtg gaaaacctcc
      481 cagccgtcgg cctgcagcag gtcggccact atgccggccc ccagctcctc ctggtcccca
      541 accccgcacg tgaccagcac gcgcttctga ttgcgcgggc tttgtgcgag gtgcggctgc
      601 agttggccca ccactgcccg gttgatccgg cacgccatgt gttcggcggc gctgttgatg
      661 cggtcaccac gatagagccg gtctacctgt accatcgccg gccagactac gtcgcagatg
      721 agctcttcgg ccgcttgccc ggcggccagg gcatcagcga tgagggcgaa gcattcggcg
      781 cggcggccgg tgataagggg ctggaggtag cgggttagga ggggctcggg catggatgac
      841 ctcttacaaa gcccgccggc caccgccatc caggcaggcc ccgcgaaggg gggcggacgg
      901 gctgacacgc ttaccggtgc atccatgcag ccgggcagaa tttccgtcac cgaagcctat
      961 cgggagaggc gagcagcgcg gcttaaaggg aaggggggta agacgcaggg cttgcggcgc
     1021 aaaattcgca ggtagcggga taacgcttta ctgattatgg gcccagtttc atgctaaccc
     1081 cccgcgtttc ctgacggttc ggccgtagcc ttcgaccggt ttcggccgga cttctttatc
     1141 agcgacgcca ccacgccggc caacaggacc gccagaacca cggccaacag gtaaaggtta
     1201 aagtgccgcc cgagcaggtc cttcagccac gtgtgcgcca acatcttcac gccgacgatc
     1261 accagaacga ccgccagggc cactttgaga tactcgaaac ggtctatgat gccggccagc
     1321 gcgaagtaca ggctgcgcag gccgaggatg gcaaacacgt tgcttgtaaa gaccaggaac
     1381 gggtcggccg tgatggcgaa aatggccggg atcgagtcga ccgcgaaaat caggtccgta
     1441 aattccacca tgatcagcgt cagcagcagc ggcgtggcga gcagtgctcc aggccgtgcg
     1501 gcctcgacga ccgaatccgg ttcaacggcg cttcccggct gcaccggttc gcgccccgcg
     1561 gccgatccgg ccctgacgaa gaaatgctgg ccgtggaagt gctgcgtgat cgggagcaag
     1621 cgccgcgtcg cgcgaacgac cacattgtgg ccagggtctg cggcggccgg cttgctgaac
     1681 agcattcttg cgccggtgat gatgaggaat gcgccgaaga cgtagatgat ccaggcgaag
     1741 cgatagatca actgcactcc gatggcgatc attgccccgc gcatgagcag ggctccaacg
     1801 atcccccaga acagcacgcg atgctggtac atggccggta cgccgaaata cgtaaaaagc
     1861 atggcgatga cgaagatgtt gtctaccgcc agcgatttct cgaccagata gccggtgatg
     1921 tacttgactg tggcggagta gccgtcgttg tagacagtaa ggccgtcctc gggcgcttgc
     1981 gggacgtggc tcatcatgtc ccaggtcagg ccgaggccca gccagtggtt ctcgtagccg
     2041 aagtaaatga acacgctgaa cgccagcccg agcgcgatcc agaaggctga ccaacccagc
     2101 gcttccttga tggagatgac gtgcgccttg cggtgaaaca cgcccaagtc cagcgtcaga
     2161 agcagcagga ccagggcgac aaaaccggcg tacatccaga acattgacaa ctccgatcca
     2221 gcacctcgtc agccttggct ggcgcatgat gcccgcgcac tatcgcctgg agtattgacg
     2281 cgcgccaacg caagcacaaa tgcaatttta ggatgccgcc gcgacggatt acgccgcttc
     2341 gagccggcga gcgtgagacc ggttccgaac atggttcaag tgccggcggg ggaattggcc
     2401 ggcgggtaga cagtgcggat cgccgtcagg atggtccgcg tcgcgggcga tttgttcaac
     2461 gtgtaaaagt gcagtccggg cgcgccgcgt tgcaacagtt cgatgcactg agccgtcgcg
     2521 tgtgccacgc ccatcgacag gaccgcgtgc gggtcgtcct gcaaacgatg caactcgttc
     2581 acgagtgttg ccggtatgct ggctccgcag gtgcgcgtga agcgcttgac ctgggccacg
     2641 ttggtgatcg gcatgatacc ggggatgatc ggcacgccga tgccggccgc ccgcgcccgg
     2701 gccacgaagt cgaagtactt gttattgtcg aagaagagct gcgtgatcag tacttcgcag
     2761 ccggcgtcca ccttgcgctt caggttgtcc aggtcggcct gcaggctcgg cgcctcgacg
     2821 tgcccctccg ggtagcacgc cccgagcacg cagaatttgg agtgcttgcg ggccagggcc
     2881 accagttcgc aggcgtatcg aaaaccctgc gggtgcggct ggaacggccc cgtgccggtc
     2941 ggcgggtcgc cgcgcaacgc caacaggttc tcgactccgc cgcgttcgat attctccagc
     3001 aggcttcgca gctcgtcttg cgacgaaccg acgcacgtca ggtgcgccat cgcctccagg
     3061 ccgtaatcgc gtcgcagctc cgtcgtcagc cggatcgtct cccgccgcgc cgcccccgac
     3121 gcgccgtaag tcacggagac gtaagtcggc cgcaagtcac gtagcgacgc cagcgtctgg
     3181 cggagtcggt caaacccggc ctcgtccttc ggcggaaaga actcgaagga aatgctgggc
     3241 cggccgtatg acaacagttc gcggatgcgc atggagctcc ttcggccgcg gtgagagttt
     3301 cgccctcggt cgccgcggag tcaatgcggg cggtgcgagg ggggccgggc ctgtcgtccc
     3361 ggaataccgc caagagaggg atcaagggat ctagggatcg agagatcgag acgaacgaag
     3421 acagagccag ggcgtgccgc cccccggaat accgccaacc ggcattccgg cgggttcagg
     3481 gggcaggtgg tcaagcaggt gatcaggcgt ctcataaaaa cgtcgtgcgg ggtagcgcgg
     3541 ggggcaggtg cgcaggtaat catgcattgt gcactcgatt ttgataatgt gccgatgact
     3601 ggggcgaagc tatcagcttt cagctctcgg ctatcagccg tcgcatttag aaacgggggc
     3661 gcgttcagaa acgccgccct tgttcgcggc atggtgacag tttcatagtg ggccccgcgt
     3721 ttgggccgga atgccagttt gccagattgc caatcctggt tggctatggc cgataacagc
     3781 ggtgtttgga ttgcgccagg ggcaggttgc cagtttgcca aggtatgcgc gctcgatttt
     3841 ggcaaagtgc cgataacgcg ctgcgcgaga gcacgctgcg cgagagcagg agacaggtga
     3901 acaggagacc ggtgagctgg agaaagtagg gggcggaggg cgggaattag gaatgacaga
     3961 ttcatgcgag gcctttctgc gcgcggcgcg gttctgctag atattctata aagggggcag
     4021 ctttcagcta tcacctgtca gccggagggc gcggattgtt ggcgtttgct ggccaatggc
     4081 ggggagacgc tgcctgaggt gatagagtgc taaggtggcg agttgttgaa actcaggaca
     4141 cggggacgca gctggtgcct gccgcgcttc gaaccgcccc gaatctccgc atcgtccagg
     4201 cgcaccggga cgaaccgccg gtccttgttc agcggatccc gaaatcgaaa cgtctggctt
     4261 tccagcctcg cccaatctga cccaaaggca ttcgccgaca tgcacagcac aagcacgcgc
     4321 gagttttcaa tcccctcttc aatcttcgcc ggaatgttgt cgccgggctt gatcacccac
     4381 tcatcgaacc acaccttcag accgtcgctg cgcagccgct ccacaattgc gcgcaccgct
     4441 tctcggtctt tcgagctgta acttagaaaa acgtcgcaag cgaacgcaga catatgctcg
     4501 ccctctgccc ggcgtgaacc gtgccctgcc ccagtgacgc gccgacaggc cgacaattcc
     4561 gatccggaat cctaccgcgc cctcgccccg cgaccaaacc gccatggtca ccacacagca
     4621 acaacgtatc acaacaaatg cgactgagtc tgtcgccaat cgaggctaca agtgcggaag
     4681 tctgttgcgt tctccaattc atacaagact tgccgccgcg ttttctttgc gtcctctgcg
     4741 ggtcgcaatt accttccccg tccttcgtgc tttcctgggt gtagggagaa aagctggcgg
     4801 gcatgaaggg ttcgcccgcc ctgccgggcg ggcgggcggg aggaaggggt ggggcctcgg
     4861 ttttccaggg gctgaagccc ctggctatcg ctcgtgcgcc ctccgggcga ggacagagnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc caggggctga agcccctggc
     5041 tatcgctcgt gcgccctccg ggcgaggaca gagccgccgg catcgctcgt gtgccctccg
     5101 ggctaggaca gagccgccgg catcgctcgt gtgccctccg ggctaggaca gagccggcgg
     5161 catcgccaag gttttcaact acaccccttt cctattcccc cacgtcgacc actttccttc
     5221 ttttcatcgc cctggcgacc cgctaagatg cggtgccttt tacttgcggc gccttttcgg
     5281 cggctcttta gattcttggc tcgggcgatc gccccggagg cataaatgaa gatactggtc
     5341 gtcttaaagc aggtcccgga ttcgaccacc aacatcaaga ttcgccccga cggcgcggac
     5401 atcgaacgcg ccggcgtcaa aatggtcgtc aatcccttcg acgaattcgc catcgagcag
     5461 gccgtcctgc tgaaggaaaa acgctcggac gtgcagagcg taaccgcgtt gatcgtcggg
     5521 cccgccgcgg ccgccgaggc cttgcgaacc gcgctcgccg tcggcgccga tgacggcatc
     5581 cacctgcaag acccggcctt cgaccagctc gacgagctgc aaacggccgc cttggttgcg
     5641 gccgtcgtga aacctcgcgg ctacgacctg atcctcaccg gcaagcagga aatcgacctc
     5701 gacagcgggc aattcggccc ggccctggcc gaagccctgg acatcccgca cgtcggggcc
     5761 tgcaccaagc tggagatggc cgccgacggc aagtcgttta cggccaaccg ccgcatcgag
     5821 ggggccgagg aagtcgtgga ggtcccgctt ccggcgctga tcaccgcgga aaagggcctg
     5881 tgcgaaatgc gctatccctc cttgccgaac ctcatgaaag ccaagaagaa gccggtcgag
     5941 acgctcaagg ccgcagacgt gccgggcttc aacgacctcg tctccgcggc cggcggcatg
     6001 aagctgcaca cgttccgtcc accgcccgag cggccgcctg gaaaggttct caagggcgaa
     6061 cccgagcagg ttgtgaagga cctcgtgaaa cttctgcggg aagaagccaa agccctgtga
     6121 gtgccgcccc cgcgtgcctg gcgactgacc actgaaaact gacaactact tagctgcgga
     6181 aagctgaggc tccgatgacg aacgacatcc tgatcttcgc ggaacagcgc aacgacaaaa
     6241 ttcacccggc cgccctgcaa atcgtcacct gcggcagagc cctggccgcc aagaccggcg
     6301 gcaaggtggt ggcctgcgtc ataggcgacg cgaccggcgg cgcggccgat gccatggaca
     6361 aggcgggcgt ggacggcctg gtgatcgtca acgatccggc gctggcccaa tactcggccc
     6421 tgcgctaccg cacggcgctg tcggccgtca tgcaaaaggt tgatccgcgg gttgtcctga
     6481 tgccggccac gttcatgggc cgcgaccttg cgccgcgcgt ggcaatgcag cgcaaggcgg
     6541 ggctggcggt tgacgcgatc gacgtgcaga tcggcgcgga cggcgcgctg gacgtcacgc
     6601 ggccgctgta caacggcaag gcgacggcgc acgtggcctt cgccaaggac cggccggcga
     6661 tcgtctcggt ccgcgccaac acgttcccgg ctccgagtag cggcgcaggc ggcgctgccc
     6721 gcgagagcgt gccgtaccag gctgccgcgg gcgacgagcg catcacggtc aaggaagtgc
     6781 tcaaggcggg cggcgaagtc aaggacgtca ccgaagccga catcatcgtc gcgggcggcc
     6841 gggcgctgaa gaaccaggac aatttccaga tgctctatga cctggccaaa gcgctggacg
     6901 ccgcggtcgg cgcatcgcgc gccgcttgtg acgcggggta tcagccgcac agccgacagg
     6961 tcggcctgac cggcaagacg gttacgccga agctctacat ggcattcggg atcagcggtg
     7021 cgattcagca cctggcgggc atgcgcggca gcaagacgat tgtcgcggtg aacacagacc
     7081 ccgaggcccc gctgttcaag gtcgcggatt acagcgtcgt gggcgacctg ttcaaaatca
     7141 tcccgctgct gaccgaggaa gtgaagaagc tgcactgacc gttcaggtca tcagcggcac
     7201 cctctggtca gttgttgccc tgcggcgccg ggtccgcgcc gcggcggcat ggcccggcgc
     7261 ctgaccgcga attcacgtcg gaatcagggc gatttcaccc gcttgcggcc ggagaatccg
     7321 ttctggcggc aagagaatct tttggcgcgc cttgatcgat cttcgaaaag ggagcacaag
     7381 ccatgccgca gaagctcgac ggactcaaag tggcaattct cgttactgac gggttcgagc
     7441 agattgaaat gacctccccg cgggacgcgc tgaagcaggc cggcgctcag ccagtgctgg
     7501 tttcacccaa tcacgatcgc gtccaaggtt gggaccatca ggacaaggcc gacatattcc
     7561 cggtcgacct gccgctggac cgcgccgacc cgaacgactt cgatgcgctg ctgttgccgg
     7621 ggggcgttgc cagccctgac aagctgcgca tgaaccccaa ggcggtgcaa ttcgtgcggg
     7681 cgttcttcga ccagcacaag ccggtcgcgg ccatctgcca cggatcttgg acgctgattg
     7741 acgccgacgc cgttcgcgga cggcgaatga cctcctggcc gtcgttgaga acggacttgc
     7801 ggaacgcggg cgcgacgtgg gtcgacgagg aagtggtagt ggacggcaat ttgatcacga
     7861 gccgcgcgcc ccgggatctg ccggcgttca accgcaaggt gattgaagag tttgcggccg
     7921 cccttgccgg cgcccgaagc taaggtgata tggtgatatg gtgatagggt gataaagggg
     7981 ccccgcgaaa tccccttatc accctatcac gttatcactt tatcactctg gccgagttac
     8041 gccggcgtgg gaatcgggcc cggctcttcg cccgcctgcg gccgatgcgt gacggggcgc
     8101 gccagaggcg cctccagccg ccggttctgc tcggccgaga tcaggtcggc caccgtcggg
     8161 cgatttagag actcgccgtt gaccagggcg cgcacttctt cgccatcgag ggtttcgtat
     8221 ttgagcagcg cccgcgcgac cgcttcaagc ttatcgcggt tttcggccag gatgcggcgt
     8281 gtgtcggcgt aggcctcatc gatgatccgc ttggtctctt catcgataat ttccgcggtt
     8341 cggggcgaaa ggtccttgcc gcccaggctg aaatccggcg cggtgcgggt cgagagcggt
     8401 tcatctccac agtacacgaa cccaactctg tcgctcatgc cccattcggt aaccatgtgc
     8461 cgagccatca tcgttgcctg gcggatgtcg gccgcggcgc cggaattcgt gtcgtgcccg
     8521 accaactcct cggccagccg gcccgcgcac aagacgcgca acgtagcctc gacgtgtgac
     8581 cggttgtaat tctggcggtc cttttcgggc agcgagaagg tcgccccgcc atacggcccg
     8641 cgtggaatga tgctgacctt gtgaaccggg tccgcgtcct tgagcagcgt ttgcacgacc
     8701 gcatgccccg cctcgtgata ggcggtcatc tcgcggtcct tctcctcgac tacgcggctg
     8761 cgcttggcgc gcccccaacg gaccttgtcg cgcgcctctt ccagatcgtc catgtcgatc
     8821 gcgtctttgc ccgccagcgt cgccagcagc gccgattcgt tgatgatcgc ggccaggtcc
     8881 gccccgctga acatcggcgt ggcgcgggcc agccgctgta atttcgtgtc gaccggggtc
     8941 tgcattttca ccttccgcgc atgcacgcgc agaatctcga accgcccctt cacatcgggc
     9001 aatggcacgt acacctggcg gtcgaagcgt cccggccgca cgagtgccgg atcgagcacg
     9061 tccgcccggt ttgtcgcggc aatcacgatc acctggtcgt tgctctcgaa gccgtccatc
     9121 tcgaccagaa ttgcgttcag cgtctgctcg cgctcgtcat gcccgccgcc gttgaagccc
     9181 agtccgcgcc gccgcccgac cgcgtcgatt tcatccagga agacgatgca gggcgagttt
     9241 tccttggctt ggcggaatag atcacgcact cgagaagcgc ccacgcccac gaacatttcg
     9301 acgaagtccg aaccgctgat ggagaagaac ggcacgtctg cttcgccggc aatcgctttt
     9361 gccagcaacg ttttgccgca ccccggttcg ccgatcagca gcacgccgcg cggaatccgc
     9421 ccgcccagcc gctgaaagcg cttggggttc ttcaaaaact cgacgacctc gtagacttct
     9481 tctttggcct cttcgatgcc ggccacatcg gcgaacgtga cgttggtgtg ctccttggtg
     9541 gtcatccggt gccggctgcg gccgaagttg cccagcatcc ccggcccccc gccggtctgg
     9601 cgcaattgcc ggaacagaag gaaccacacc acgccgaaca gcaccagcca cggtaaggtc
     9661 gtgacgagca aattcatcag caggctgttg ctgggttcgc tcttgatagt taccgggcgt
     9721 ggactgtcgc tctcgatagt cttgcggatt tcctcgacct tatcggccgc tttgaggtag
     9781 gtacactcga agcgcttgct gtcgccttgg gcatgggacg gcaacctctc gaattcgccg
     9841 atgatcgaat cctcgcggat cgtcacctcc ttcacgttgc cgttgcgcac ttgcgtccag
     9901 aattcggaga tggggatttt ctgcccaggt gccatgccgc ggttgaacat caccagcagc
     9961 gttatcacca gcatcaccag caggacccaa ctaaatgcgc cgcgcgtcag acgcggcggg
    10021 cgaggggggc cgccgtttgg ccgtgattcc tggttgggct tgagatcctc agacatccgt
    10081 tttttcgttg gtcgcttgaa ggggccgaac accgatccgt caccattcct cgtacatttt
    10141 ggctacaatc cgtgccgcca acagatttat cgcccgttct tgggccaggg cttccggctg
    10201 accctccggc ggcacgtaat cggccgcctg caactcgacc ggctggtcaa tcagcacccg
    10261 accggagcgc aaatccttcc attgcacgcg ggcagccagg accagaacca tctcccgtgg
    10321 ttggcgggag gcatagtctt gggcaaaggc cgcctggcgc tcctccagca actcgccttt
    10381 caatatcgta tccgcctttt ccggcggggc cagccgataa ggcgtatcga ggttaatgcg
    10441 tttcttgatc gcctcggtta gcttaaactc caggtcgcgc cggaattcct tgctgccgaa
    10501 catgtccacg tacaccgtct gaacgtcgca gcgatgaatc cctccgtggc gatagccgca
    10561 gccgacggtg ccgcccagcc ccagcgtgac caggactatc aagcagaact gctgtccctt
    10621 cattccgcgt cctccacctc gacccccagc gtccgcagcc gctgacgcgc ttccgccgca
    10681 gccagcgtgt cgccccaatc gcttagaacc agccggtaat agtattcggc cgccgatttt
    10741 tgtcgcgtgc gctcgtacca cttcccgatg tccaagtcct tggcggcccg ctgctggcga
    10801 atgccctcca gccgctgggc gacgttttcg cgctccgcgt aaatcgggta cgccgcctga
    10861 acctggcggt aacgctcgtc tgcctccagc agcggccggt catcgaattt gattccgggg
    10921 aaggccgcct cggccgccac ggcgctgcgc atcatggcga attgcgtgta ctttccggtg
    10981 gggaactggc gcgccaggtt tgcgtactcg tcctgcgcca ggtccatctc gccttggctg
    11041 aagcggtaat cggctttggt acgcagcgcc tcttcaccca ggcgcgtgcc cggcacgcgc
    11101 tcccaaatgc ggtcgaggat atcgatcgcc tcgtcggtgg ccgggaatcg aaagatgccc
    11161 catacgacac gcttctcgcc cgacaggaac gccaaggcca cgtcgcgttc gcggaacagg
    11221 gccgtgtgga acaactcgcc gccggtgttt tcgacgacca gttcgtactg ttcataggcg
    11281 cggtagtaat ctttcgactc gaaccacgtc tcgcccagca ggaaaacggc ctcgtagtac
    11341 cgttcgtgtc cctcgttttc cttgatccat tgctggagaa tctttcgcgc cttcttcggc
    11401 tgcccctccg ccagcagggc ccgagccgac gctaactcat cgctcgcccc gtcctccgcc
    11461 agttcctccg tgacccattc gcctatctct gggtcgagca cctcccgctc gcggtagtgc
    11521 tgatcttcgc cggctttcgg agcttccggt tggccagcca tgtccggcag gctcggctga
    11581 tcttggctct gatcgttctg agccacgccg gcctgattcg ccaagtccgg cgctatgctc
    11641 gcggtttctc cggccggcgc tgcaactgcc agcgccaaag ccgccgcaag agcaccggcc
    11701 gccgccctca cactaatccc agaaaggact gtcgatttaa tgcgccaagg attcatgacc
    11761 ggcattataa attgccgagc acaggatggc caacgaccgg gacggggtgt tgtgtccaag
    11821 ccgcgtagcg cgcgggcatg ctttcgttac cggcgccaca tacggtgact aacgcatgcc
    11881 gtcgctgacg gcgtcaggcg cagggcgact gacgcaggca agcgctgaac agccgggcag
    11941 gggggaacaa ctggtgatgg catgcccgcg cttacagctt gggcatggca cccaactgat
    12001 ctcgccaccc aactggtgtc gacaccccac tgatctcgtc acgcaatcga gcgttacccc
    12061 aactggtgag ctcgtgagcc ggccgctctt ggcggatcgt gagttttgac acatcggatt
    12121 gataaaactg ttctaacgca taccctcagt aggagtccgt tcgatgacag caccgtttta
    12181 cgacaacgaa tcggactcgc ccgcccagcg gatcgtgaat ctgctggcgc ggcgcgggcc
    12241 cagcacggtc acgcaactcg ttgctgagct cggggttacg acaaccgcgg tccgccaaca
    12301 ggtaaaccgc ctggtggccc gccgttggct gattcgcacc cgccgccgcc agggtcccgg
    12361 ccggcccgcg gacgtgttcg ccttgtcaga cgatgcgcgg cggatgctcg ccggccaagc
    12421 cgaggagctg tcgcgattgc ttctggagca gattgccgag atgccagggc gggaccggcg
    12481 gcgcgccttg ttgcaggccg tcagtaaccg catggcgcag aacgcgcagc aatttgtcgg
    12541 cgatggcccg ccgggcgaac ggctgcggcg gctggcagat cacttaagcc gcgaaggcgt
    12601 gctggcggag gccgagacgg ggcgcggcgc gcaacgcctg accgtgtaca cctgcccgtt
    12661 ttacggcgtg gccgcagcgc accgcgaggt ttgcgatatg gagcgggcgg cgttcagcac
    12721 gctgctggga gatgcgctcg cactggagcg ctgcgttctg gacggccacg aacattgcga
    12781 attcaggccg cgcggcgcgg ccagaggcgc ggcccgcggc aaggacgccg gcaacagggc
    12841 cggtaaggag tcgcttgaat gacgaagatt gcggatccgc tccaaacctg ggcgcaatct
    12901 gaatacaagt atggttttgt caccgatatc gagacggaaa ccgttccgcg cggactgagt
    12961 gaagaagtta ttcgcctcat ttctgcgaaa aagcacgagc cggagtggct cacgcaatgg
    13021 cggttgaagg cttaccgtca ctggctcaca atgaccgagc cgacctggca gaacgtgcat
    13081 tatgcgccga tc
//
LOCUS       DAOSYS010000084        13015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4032_length_13015_cov_5.144907, whole genome shotgun sequence.
ACCESSION   DAOSYS010000084 DAOSYS010000000
VERSION     DAOSYS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 13015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13015
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4032_length_13015_cov_5.144907"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLU99_06950"
     CDS             <1..281
                     /locus_tag="PLU99_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16831.1"
                     /translation="GTSSPPENVVSLFSMSGRIELAQQRRICADGQSCAHPQRHYFVA
                     RVGENVTVIKWDEAQSSLVVCSCIPQEELVELVDSTKLAQSQVAQLAD"
     gene            complement(330..518)
                     /locus_tag="PLU99_06955"
     CDS             complement(330..518)
                     /locus_tag="PLU99_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16832.1"
                     /translation="MAVTLFAGILTLFGCATLTETPEEYCYVQRRAIKMSVLQIPEDA
                     RYIMLLDRPSRLSRWMLK"
     gene            complement(686..1735)
                     /locus_tag="PLU99_06960"
     CDS             complement(686..1735)
                     /locus_tag="PLU99_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOO16833.1"
                     /translation="MTPRQLGYRMPAEWEPHQATWVSWPHKEASWPGKLETVFPVFAR
                     MVAALAASETVHINVCDQDMEQRARAFLQQAEAVGDIRFHHFPTNDAWCRDYGAIFVR
                     RAAGQGSRGAGELAATDWGYNAWGDKYPPYDLDNKIPALMAEVLGTPRFVADMILEGG
                     SIDVNGQGVLLTSEACLLNPNRNPQLGRAEIEERLREYLGVEHILWLGEGIVGDDTDG
                     HVDDLARFVDPETIVTVIEDDPADENYRPLQDNLKRLRSFRQPDGRPFRIVELPMPRP
                     IVWEDQRLPASYANFYIGNKVVLLPGYSDPNDRVAEERLAALFPGRRIVTLDCTDLIW
                     GLGAFHCLTQQVPCA"
     gene            complement(1732..2643)
                     /locus_tag="PLU99_06965"
     CDS             complement(1732..2643)
                     /locus_tag="PLU99_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HOO16834.1"
                     /translation="MNAENHHVSVGLVQMRCAPDPAANVESAAKLVRQAAAQGAQIIC
                     LPELFRTQYFCQTENHANFDLAEPVPGPTTEALAALAAELSVVIIASVFEKRARGVYH
                     NTVAVLDANGAYLGKYRKMHIPDDPLYYEKFYFTPGDLGFRCFDTRHARVGTLICWDQ
                     WYPEAARATALHGADILFYPTAIGWHPSEKARWGAQQLENWQLIQRSHAIANGCFVVA
                     VNRVGHEGPADGGIEFWGSSFVAGPSGDIRVRAAVDAEQVLVSQLDLGDIERHRTHWP
                     FLRDRRTDAYRDLLSVYIGEQHKGEPV"
     gene            3077..6229
                     /locus_tag="PLU99_06970"
     CDS             3077..6229
                     /locus_tag="PLU99_06970"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOO16835.1"
                     /translation="MSQKEAADLRLTEEPLLTPNARTVLEARYLKKNEAGECIETPGQ
                     LFRRVARTIANIEPAYGADETERQTWEQRFYDLMASGKFMPNSPTLMNAGRELGLLSA
                     CFVLPVHDSIVDIFDAIKQTALIQKAGGGTGFAFDELRPTGDYIKSSGGTTSGPITFW
                     RAFSEATNAIQQGAFRRGANMGMMYIHHPDILKFLYAKQDLNQFTNYNISVKVTDTWM
                     EEFRADPDSPHIVRNPRTGRAYAIPRAVDIWKHDLRDLVEIHCVEPASGRAAYYTTPR
                     LTSPLPEELQGHIYTKHDIWTIITQHAWQTGEPGIVFIDRINQHNPTPHVGRIEATNP
                     CGEQPLLPHEACNLGSLNLGRFVRNACTPQAEVDWEAMRQAVRDSVRFLDNVIDANTY
                     PLPEIEKMCKANRKIGLGIMGFADALFALNVPYNSALGVSWGERFMKFINEEAHLCSE
                     ELARTRGCFPNWQGSIWQTRYDRPMRNAAVTTVAPTGTISIIANCSGGVEPLFSLAFI
                     RNVLRGQKQGEKPLVEVNALFRRVAQERGFLSDELLERLAAEGTLARIPKVPEDVRRV
                     FVCAHDISPEWHMRMQAAFQQHCDSSISKTINFPRSATVADVERIYHLAYELDCKGVT
                     VYRDGCRAGQPMALKNGEGEKAHADASEGASAPPSDSAASRPVREQAQCEACPEGTPP
                     SVEPTNRPGGPPTRPYIEPADLPEIVSGFRIRQMTPFGNMHVKITVDPHSQRELEIFA
                     QLGKGGDVANSDLEGLCRIASLWLRAGGSLVNLIKQWEGIGSSLQIPTRAGRIMSLPD
                     GLACALKKYLRAKERFGLRALLLGEIDPAELDNPYPPPHERAAHAPDRTVDATRPQQA
                     PLPPKRGQDPSAGGGADSKSYATGAVQRRLDGGNVARLDSATEPKASAHSSSPTAGSK
                     PSVKSDGNGHGKGNGKGNGGTVVTTTSLGNGDSAWSGYRPPYATRSDSATALAVEEAA
                     GVAVVAEPQPQQRAASAAGHSEGMSAVERAMLASILSGGRHDHDAASHYALKCPMCGG
                     PVARQEGCLHCGNQCGWAAC"
     gene            6535..7317
                     /locus_tag="PLU99_06975"
     CDS             6535..7317
                     /locus_tag="PLU99_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16836.1"
                     /translation="MKGSGALAAPITPTPPDGGILQDQAQYQPATFTPPPSKPKESAA
                     GEDGAAQDSNEAAAVREFMFDVMRDMQAGRFERVLSKIDPDPGEAAREHMQRTLEMVT
                     ALARSVTEKLGLDSLEQFYEGELKALADNIEVELTGPDSATVTPNLLGGLLLGPDFAT
                     PKAKLVKTGGEWRFLLETPLTGADLDKLAVYHQTVQAQLKQVSEVIQQAGPAELEAIR
                     EGVMQALAAASIAELSSAPAASQAAPASAPSEEPNEGGRPAP"
     gene            7367..7795
                     /locus_tag="PLU99_06980"
     CDS             7367..7795
                     /locus_tag="PLU99_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16837.1"
                     /translation="MAVVSREQLDSLIAAIHSHDFLRRMLESMEQHHRLVFHANEHAD
                     WNLVRATAEQILVAEIVSRHKGNIDGVYFALRDLEAGGRTWEAAINELAGRVHSYYTT
                     PLGVLMRKNLFGENAVFLTTDAHDWIRRQEASSGMLGNQA"
     gene            7792..8799
                     /locus_tag="PLU99_06985"
     CDS             7792..8799
                     /locus_tag="PLU99_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOO16838.1"
                     /translation="MNANAPKARCNVADLKAGQRLDDQIFRIAQKELRTTSNGSLYIH
                     AVLADATGQILGRMWNASQEIFDSIPNGGFLAFRGRVESFKGAPQFIIDGLRCVEAGS
                     FDPSEFLPATERDVEAMWEELKTTLRQVQNRDLLALVAKFVNDPAFQTGFKRAPAAMS
                     FHHAWLGGLLEHTLNLLRLARVVCPLYPQVCQDLVLVGVFLHDIGKVRELAYETNFEY
                     TSEGQLIGHITQAVLWVHEKCAEIARETGQPFPHDLEMALQHIILAHHGKYEFGSPRL
                     PATAEAIMIHYLDNLDAKLNMVFEAIEGDTDADSEWTAFVRALETKIFKPDILGTRKK
                     T"
     gene            8987..9607
                     /locus_tag="PLU99_06990"
     CDS             8987..9607
                     /locus_tag="PLU99_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HOO16839.1"
                     /translation="MTSAPERIQMANYRSEIGRKSVHFLSALTPLVYLLIDKRLALSI
                     LVPIACAVLLIDVLRQAHPGLRAFYNRHWGHLMRGDETGRLSGASHVMIASVLCIVVF
                     PKPIAITALLFMSVSDALASLAGMRSNGPRWFRASLAGSSAFLISAAAMALLLLREHP
                     LAALVGAVVATVIEALPLSIGKARIDDNLTVPLGAGLVMWALCGWC"
     gene            9839..12022
                     /locus_tag="PLU99_06995"
     CDS             9839..12022
                     /locus_tag="PLU99_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16840.1"
                     /translation="MSRTIFLCLALMCSCVLSFGAVGAERGGNYLIVSAPDYVNTPPL
                     NQFIAHREARGFNVMVYSPPSGTSKEAIKAYIQSLWGTADQPAYLLIVGDTDGTSTST
                     NSTIPHWIGTGSKSAPTDLPYVCMDSVPVSWYPDFYLGRFSVRDLSMLNAVVAKTIAV
                     ESGGFSDPGYVRRAALLANDDSTTHSIELHDWIISTYLEPAGFTPHRIYAAYGGSTAD
                     VSAAVNAGVLFTVYFGHSGSSGWWSPAFYQADVQNLTNNGLYGLTMGWSCSSAYYPTS
                     ECFGETWLRVANRGSAAYLSSATSVWWGSDSSWESSRRMERYIFQSFFADNIWEVGPA
                     WHAALGRMYNDPEFGPAHDHTHNIFEMMVLLGDPALRLPFRALDISLMEGVPEFIHPD
                     EPTIFTVNIGSAAEQYVADTALLHYSLNGEDFQTAPLVHLGGSEFLASLPAPGCGSTP
                     RFYFSAQGDQGSTVYLPYLAPASCFTAEVAAITTLLDDDFEADTGWTVWSDPAMVSGM
                     WQRGIPVSPVPEGAPKADFDGSGSCFLTDNRSPNYDVDLGPTILLSPIINLAGRPDPY
                     VRYSRWMYCDDVIAPARDYLLVQCSSDGGANWTTVEQAWDDGAWVQREFRVRDFVTPT
                     AQFQLRFSVADNPNNSVTEAGVDAVWVYDRGCGDVPPVLGDLNCDGNVNVFDIDPFVL
                     ALTDPAGYALAFPACDAMNGDANQDGEVNVFDIDPFVALLTSSAP"
     gene            complement(12101..12679)
                     /locus_tag="PLU99_07000"
     CDS             complement(12101..12679)
                     /locus_tag="PLU99_07000"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOO16841.1"
                     /translation="MPKPALILASASPRRRELLRTIVPAFEIRACPLAEPDAKPESVN
                     PRGWAEALAYFKARAVAEANPGRLVLGADTIVTCGQHLLGKPRDLADARRMLLLQAGR
                     RCDVITGVSLVRINGRPRRLFGADVTAVWMRDDAAEIEAYLKSGEWRGKAGAYGIQDC
                     GDRLIERIEGSFPNVVGLPVELVAKVLERSAI"
     gene            12735..>13015
                     /locus_tag="PLU99_07005"
     CDS             12735..>13015
                     /locus_tag="PLU99_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16842.1"
                     /translation="MPKVKATRTIRTRRSHAPDKRRSATAHRFGAHTSIAGGMHHALE
                     IGLRLGCDTVQVFVKNQRQWRAAPLRADDLRCWNALLDTPGFGPVIAHAT"
BASE COUNT         2459 a         4184 c         4028 g         2344 t
ORIGIN      
        1 ctggcacaag ctccccgccg gaaaacgtgg tttcgctctt ctcgatgtcc ggccgaattg
       61 agctggctca gcaacggcga atctgcgcgg acggtcagag ctgtgcgcac ccgcaacgcc
      121 actactttgt tgcacgtgtg ggcgagaacg taaccgtgat caagtgggac gaagcccaat
      181 ccagcctggt ggtgtgcagt tgcataccgc aggaggagtt ggtggaactg gtcgattcaa
      241 caaagctggc gcaaagccaa gttgcgcagt tggcagactg aacggcgcca aaatcggacg
      301 cggcgcgagc gccggcgagc caggggtggt tacttgagca tccagcgcga gagacgcgag
      361 ggacggtcga gcagcatgat gtatcgggcg tcttccggta tctggagcac gctcattttg
      421 atcgcccggc gttgaacgta gcaatattct tcgggcgtct ccgtcagggt ggcgcagccg
      481 aagagggtca aaatcccggc aaacaacgtt actgccaaca gggcgcgcat ctcgttcccc
      541 ttggggccaa actcagcaaa ctgcaactag ttttggcgta accggcgggc ctgtcaagcc
      601 agcaatcgcc gggggttcgg tcttcagata caagtccggc gttgcgtgcg cttctcgata
      661 ccgagatcga cgccgaaacc actaactacg cgcacggcac ctgctgcgtc aggcagtgaa
      721 acgcacccag cccccagatc aggtcggtgc agtcgagcgt gacgatccgg cggccaggga
      781 aaagcgcggc taagcgctcc tcggcaacgc gatcgttggg gtcactgtat ccggggagca
      841 gcacgacctt gttgccgatg tagaagttcg cgtagctggc cggcagacgc tggtcctccc
      901 agactatcgg acgcggcatc ggcagctcga cgatccgaaa cgggcgtccg tcgggctgac
      961 gaaagctgcg cagacgcttc aggttgtctt gcagcggccg gtaattttcg tcggccggat
     1021 cgtcctcgat gaccgtcacg attgtctcgg gatcgacaaa ccgggccagg tcgtcgacgt
     1081 gaccatcggt gtcatcgccc acaatgcctt ctccgagcca gaggatgtgc tcgacgccta
     1141 agtattcgcg caggcgctct tcgatctcag ctcgccctaa ctgcggattg cggttcgggt
     1201 tcagcaggca ggcctcgctg gtcaacaaga cgccctgtcc gttgacgtcg atcgaaccgc
     1261 cttcgaggat catgtcggcg acgaaacgcg gcgtgcccaa cacttcggcc atgagcgcgg
     1321 gaatcttgtt gtccaggtca taaggcgggt acttatcgcc ccaggcgttg tagccccagt
     1381 cggtcgcggc cagctcaccg gccccgcggg agccttgccc agcggcgcgg cgaacgaaga
     1441 tcgccccgta gtcgcggcac caggcgtcgt tggtcggaaa gtggtggaaa cggatgtcgc
     1501 ccaccgcctc cgcctgctgt agaaaggccc gggcccgctg ctccatgtcc tgatcgcaaa
     1561 cgttaatgtg aacggtctcc gacgcggcca gcgcggccac catgcgcgcg aacaccggaa
     1621 acacggtttc cagcttgccg ggccaggagg cttctttgtg aggccaggaa acccacgtgg
     1681 cttgatgcgg ctcccattcg gcgggcatgc gatagcccag ttggcgcggc gtcatacggg
     1741 ctcgcccttg tgctgctcgc cgatatagac gctgagaagg tcgcggtagg cgtccgtacg
     1801 gcgatcgcgc aaaaacggcc aatgagtgcg gtggcgctcg atgtcgccca ggtcgagttg
     1861 ggagaccagg acctgctcgg catcaacggc cgcgcgaacg cgaatatcgc cgctcgggcc
     1921 ggccacaaaa ctgctgcccc agaactcgat accgccgtcc gctggacctt cgtggccgac
     1981 gcgattcacg gccaccacga agcagccgtt ggcgatggcg tgcgagcgtt gtatgagctg
     2041 ccagttctca agctgctgcg cgccccacct ggctttttca gacggatgcc agccgatggc
     2101 cgtcgggtag aagaggatat cggcgccgtg caaagcagtt gcgcgagcgg cctcggggta
     2161 ccattgatcc cagcatatca gcgtaccgac ccgggcgtgg cgcgtgtcga agcagcgaaa
     2221 gcccaggtcc cccggcgtga agtaaaactt ctcgtagtaa agcgggtcgt cggggatgtg
     2281 cattttccgg tacttgccga ggtaggcgcc gttggcgtcg agcacggcga cggtgttgtg
     2341 gtagacgccg cgggcccgct tttcgaaaac gctggcgatg atcacgacgc tcaattccgc
     2401 ggcgagggcg gccagcgctt ctgtggtcgg gccgggaacc ggttcggcca agtcgaaatt
     2461 ggcgtggttc tcggtctggc aaaagtactg ggtgcggaac agctcgggca ggcagatgat
     2521 ctgcgctccc tgggcggcgg cctggcgaac gagcttcgcg gcgctttcca cattcgcggc
     2581 tggatcaggt gcgcaacgca tctgcacaag accgacgctg acgtgatggt tctctgcgtt
     2641 catggggcct ggctccgcgg gcatttaccc ggctggcggt agtgtagtaa ttgtttggga
     2701 attcgtcatt gcgcccggcc gcttcaggca cggggctgat gaggaagagg aaatcagcag
     2761 tgtgagcccc ctgtatctcg gaaaacgact ggacaggcca ggatattcgt gttaggatta
     2821 tgtttggtgg aacgatacag gcgctgctta gtccgaaaat tccataatgc ttgcggggaa
     2881 aaaatgtcgt gcacgcaacc cacgctggcc actcgacttg cggcgattat cggctgcgag
     2941 tttgacgcat acaatatgtt gggctatatt gaaggttgcc ccctatagat ggcaggcagc
     3001 aggtcctgac cgccgctggg gcgttttcgt ctcgcggtca aacgtcagcg aggttcattg
     3061 agggaaggac ggtcgcatga gtcagaagga agccgctgat ttgaggctca cggaggagcc
     3121 gctgctcacg ccgaacgccc gcactgtcct tgaggcccgc tacctcaaga agaatgaggc
     3181 cggcgagtgt atcgaaacgc ccggccaact cttccggcgg gtcgcacgga ccatagccaa
     3241 tatcgaaccc gcttacggcg ccgacgaaac ggagcggcag acgtgggaac agcgctttta
     3301 cgacctgatg gcgtccggca agttcatgcc caactcgccc acccttatga acgccggccg
     3361 cgagctgggc cttctgtcgg cctgcttcgt cctgccggtg catgattcga ttgtggacat
     3421 tttcgacgcg atcaagcaga ccgctctgat ccaaaaggcc ggtggcggaa ccggtttcgc
     3481 tttcgacgag ctacgtccca ctggcgacta catcaagtcc agcggcggca ccaccagcgg
     3541 accaatcacg ttctggcgcg ctttcagcga agccaccaac gcgatccagc agggcgcttt
     3601 ccgccgcggg gccaacatgg gcatgatgta tatccatcat cccgatatcc taaaattcct
     3661 ctacgccaaa caggacctca atcagttcac aaactacaac atctcggtca aggtgaccga
     3721 cacgtggatg gaggaattcc gggccgaccc cgattcgccg cacatcgtcc gcaacccgcg
     3781 caccggtcgc gcctatgcga tcccgcgcgc cgttgatatc tggaaacacg atttgcgcga
     3841 cctcgtggaa atccactgcg tcgagcccgc ctccggccga gccgcctatt acacgactcc
     3901 gcggctgacc agccctctgc ctgaggaact gcaggggcac atttacacca agcacgacat
     3961 ctggaccatc atcacccagc acgcctggca gacgggcgag ccgggaatcg ttttcatcga
     4021 ccggattaat cagcacaatc ccacgccgca cgttggccgc atcgaagcca ccaatccctg
     4081 cggcgagcag ccgctgctgc cgcacgaggc ctgcaacctc ggcagcctga atctgggccg
     4141 gttcgtgcgc aatgcgtgta cgccgcaagc cgaagtggat tgggaggcca tgcgccaggc
     4201 ggtgcgtgac agcgttcggt tccttgataa cgtgatcgac gcgaacacgt atccgctgcc
     4261 cgagatcgag aagatgtgca aggccaatcg caagatcggc ctgggcatca tggggttcgc
     4321 ggacgccctg ttcgctctga acgtgcctta caacagcgcc cttggcgtgt cctggggtga
     4381 gcgcttcatg aaattcatca atgaggaagc gcacctgtgc agcgaggagc ttgcccgcac
     4441 gcgtggctgc ttccccaact ggcaaggcag catctggcaa acgcgttacg accgcccgat
     4501 gcgcaacgcc gccgtcacaa ccgtagcccc gaccggcacc atcagcatca tcgccaactg
     4561 ctcgggcggc gtggagccgc tgttctcgct ggcgttcatt cggaacgtcc tgcgcggcca
     4621 aaagcaaggc gaaaagccgc tggtcgaggt caacgcgctt ttccgccgcg tcgctcagga
     4681 acgaggtttc ctgtccgacg aactgctgga acgcctggcg gccgaaggca cgctcgcgcg
     4741 catccccaag gtgcccgagg acgttcgccg ggtatttgtg tgcgcccacg atatttcgcc
     4801 cgaatggcac atgcgcatgc aggccgcttt ccagcagcat tgcgactcct cgatcagcaa
     4861 gaccatcaat ttccctcgct cggccacggt tgccgacgtc gagcggattt accacctggc
     4921 ctacgagctg gactgcaagg gcgtcacggt ctaccgcgac ggttgccgcg ccggccagcc
     4981 gatggccttg aagaacggcg agggcgaaaa ggcccacgcc gatgcgagcg agggggccag
     5041 cgcgccccca tctgatagcg cggccagccg gccggttcgc gagcaggcgc aatgcgaagc
     5101 ctgccccgag ggcacccctc cctcggtcga gcctacgaac cggcccggcg gcccgcccac
     5161 ccgcccctat atcgagcccg ccgacctgcc cgagatcgtc agcggcttcc gcattcgcca
     5221 aatgaccccc tttggcaaca tgcacgtaaa gatcaccgtc gacccgcact cgcagcgcga
     5281 gttggaaatc ttcgcccaac tgggcaaggg cggcgacgtg gccaatagcg atctggaagg
     5341 cctctgccgc atcgcctcgc tctggctccg agccggcggc agcctcgtca atcttatcaa
     5401 gcaatgggag ggcatcggct catcgcttca gatccccacc cgcgcgggcc gcatcatgtc
     5461 gctgcccgac ggcttggcgt gtgcattaaa gaagtacttg cgggccaagg agcgtttcgg
     5521 cctccgcgcc ctgctgctgg gtgagatcga tccggcggaa ctcgataacc cttatccgcc
     5581 gccccacgaa cgcgcggcac atgcgcccga tcgcaccgtt gacgctacac ggccccagca
     5641 ggcccccctg ccgccaaagc gcggccaaga cccaagcgcc ggcggcggag cagattcgaa
     5701 atcctacgcg acgggcgccg tacaacgcag gctggacgga ggaaacgtcg cacgcctcga
     5761 tagcgccaca gaaccaaaag cctccgccca ctcgtcgtcc ccaaccgccg ggtcgaaacc
     5821 cagcgtcaaa agcgacggta acggccacgg caagggtaac ggcaaaggca atggcggaac
     5881 ggtcgtgact accacgtccc tcggtaacgg cgacagtgcc tggtccggct atcgcccgcc
     5941 gtatgcgacg cgctcggatt ccgccaccgc gctggcagtt gaagaagccg cgggcgtggc
     6001 ggtcgtcgct gagccgcagc cgcagcagcg tgcagccagc gccgcagggc actccgaggg
     6061 catgagcgcg gtcgagcgtg ccatgctcgc cagcatcttg tccggcgggc gccacgacca
     6121 cgacgctgcc agccactacg ccctcaaatg ccccatgtgc ggcggcccgg tggcccgcca
     6181 ggaaggctgc ttacactgcg gcaaccagtg cggctgggcg gcgtgctaac gaccctcgcg
     6241 cagagcacag cgacaccggc tcacaggcat cgggcccgcg tgctctgcac cggagagctt
     6301 ccacacgccg gcgtcccggt agatttgccc cgcaaacccg gttacgcttt tgttcctgcc
     6361 gattatgtcg aggcacaccg gcgggcagcc cggcatgggc cgaggcgagc gagtacggca
     6421 cgaggttgag tgacgatgtg gagaactggc atcctgttaa ccgcgatcct gatttgcgca
     6481 acggttctga cttcgtgcgg cgattccgct gagccgccgc cgcggagcgc aaccgtgaaa
     6541 ggcagcggcg cactggccgc cccgatcacg cctacgccac cggatggcgg cattctgcag
     6601 gaccaggctc agtatcagcc ggccaccttc acgccgccgc cgagcaagcc aaaagagtcg
     6661 gccgcggggg aagacggcgc tgctcaggac tccaacgagg cggccgctgt gcgcgagttc
     6721 atgtttgacg tgatgcgtga catgcaggcc ggcaggttcg aacgcgttct cagcaagatc
     6781 gaccctgacc cgggcgaggc ggcgcgcgag catatgcagc gcacactgga gatggttact
     6841 gccctcgcgc ggtccgtaac tgaaaagctg gggcttgatt ccctggagca attttacgag
     6901 ggcgagctga aagctctggc ggacaatatc gaggtcgagt tgaccggtcc tgactcggcg
     6961 acggtcacgc ctaacctgct gggtgggtta ctacttggtc ccgacttcgc cacgcccaag
     7021 gcgaagctag tcaagacggg cggggagtgg cgtttcttgc tcgagacgcc gctgaccggc
     7081 gcagacctgg acaagctggc ggtgtatcat cagacagtac aggcacaact gaagcaggtt
     7141 agcgaagtta ttcagcaggc cgggcccgcg gaactagagg ctattagaga aggcgttatg
     7201 caagccttgg cagccgcgag catcgccgaa ttgagctcgg cgcccgcagc gagccaggcg
     7261 gcgcccgcca gcgccccgtc ggaagagccc aacgagggcg ggcggccggc tccctgaagt
     7321 cgggcgcgac ggcaagtgta aaccaggcga gtcttggagg tttggtttgg cggtcgtctc
     7381 acgagagcaa ctggattccc tcatcgcagc gattcactcg cacgactttc tgcgccgcat
     7441 gctggagagc atggagcagc accaccggct ggtcttccac gcgaatgagc acgcggattg
     7501 gaacttggtg cgcgccactg cggagcaaat cctggtcgcg gaaatcgttt cacggcacaa
     7561 aggcaacatt gacggcgtct actttgccct gcgcgacctc gaggccggcg ggcggacctg
     7621 ggaagccgcc atcaatgagc tggcgggccg cgtccattcc tattacacca cgccgctggg
     7681 cgtcctgatg cgcaagaacc tgttcggcga aaacgccgtg ttcctcacaa ccgatgctca
     7741 cgactggatt cgccgtcagg aggcctcttc cggcatgctt ggaaaccagg catgaatgcg
     7801 aacgctccca aagcgcggtg taacgtcgca gacctgaaag ccggccagcg gctcgacgat
     7861 caaatcttcc gcatcgcgca aaaggagctg cgcaccacct cgaacggcag cctctacatc
     7921 cacgccgtgc tggcggacgc gaccgggcaa atcctgggcc gcatgtggaa cgcctcgcag
     7981 gagattttcg attcgatccc gaacggcggg ttcctggcgt tccgcggacg cgtggagagc
     8041 ttcaaaggcg ccccgcagtt catcatcgac ggcctgcgct gcgtggaagc cggcagcttc
     8101 gacccgagcg agtttcttcc ggccaccgag cgcgacgtgg aggccatgtg ggaagagctt
     8161 aaaaccaccc tgcggcaggt tcagaaccgg gacttgctgg cgctggtcgc caaattcgtc
     8221 aacgaccccg ccttccagac cggcttcaag cgcgccccgg ccgcgatgtc ttttcaccat
     8281 gcctggctgg gcggcctgct ggagcacacg ctaaacctgc tgcgcctggc gcgcgtcgtg
     8341 tgcccgctgt acccgcaggt ctgccaggac ctcgtgctgg tcggcgtttt cctgcacgac
     8401 atcggcaaag tccgcgagct ggcctacgag acgaatttcg aatacacgag cgaagggcag
     8461 ctcatcgggc acataacgca ggcggtactg tgggtgcatg aaaaatgcgc cgagatcgcg
     8521 cgcgagaccg ggcagccgtt cccgcacgac ctggagatgg cccttcaaca catcattctg
     8581 gcccatcacg gcaagtacga attcggcagc ccgcgcctgc cggcgaccgc cgaagcgatc
     8641 atgattcact acctcgacaa cctggatgcc aagctgaaca tggtcttcga agccatcgag
     8701 ggcgacacgg acgccgacag cgaatggaca gccttcgtgc gtgcgctcga gaccaaaatc
     8761 ttcaagccgg acatcctggg aacgcgcaaa aagacatagg ccattcgcaa tccgcgacca
     8821 cgggcggcac gaacgggtcg atatcgtaac cgggaatgct gcggcagccc gccgccggcc
     8881 gtgttcggct gcgccgccgg cgcgcccgcc tcgccgcaga gcagtgcgaa gcttacaatt
     8941 acgacccggc gcctccggcg agccaagccg ctgaacacgg aagtaaatga ccagcgcacc
     9001 cgaacggatt caaatggcga attatcgcag cgagatcggg cggaaatcgg ttcactttct
     9061 ttcggcgctc acgccgctgg tctacctgtt aatcgacaag cgtttggcgc tgtcgatcct
     9121 ggttccgatt gcgtgcgcgg tacttctgat cgacgtgttg cggcaggccc acccggggct
     9181 gcgggcgttt tacaaccggc actgggggca tttgatgcgc ggggatgaga ccggccggct
     9241 gagtggcgcg tcgcatgtga tgatcgcgag cgtgttgtgt atcgtcgttt ttccaaagcc
     9301 gatcgcgatt acggcgttgc tgttcatgtc cgtttcggac gcgttggcat cgctcgcggg
     9361 tatgcgctcc aatggtccac gctggttccg cgccagtttg gccggcagtt cagcgtttct
     9421 gatatccgcg gcggcaatgg cgctgcttct cttgcgcgaa catccgctcg cggcgctggt
     9481 tggcgcggtg gtcgccacag ttatcgaggc cctgccgctg agcatcggca aagcccgcat
     9541 tgacgacaac ctcacggtcc ctcttggcgc gggcctggtt atgtgggctc tctgcggctg
     9601 gtgctgagcc cgccatatag tcgtctgaaa tgccggccat attataggac gctgctttca
     9661 gtccgcaaag cagtcgtttg cttcaaagtt cactgctgtc aggaatccgg cacaggccgc
     9721 gatcccggcc gccggcgggc cgaaattact tccgtcatga ccgctcgtat cgtacaattg
     9781 aatcgctccg ggggttcgga aggctgttat tgattcatcc attaggagga aggcaaagat
     9841 gtcgcgaaca atcttcttgt gtcttgcact gatgtgcagt tgcgtgttgt cgttcggcgc
     9901 ggtgggcgcg gagcgcggcg ggaattacct aatcgtctcc gcaccagact atgtaaacac
     9961 ccccccgctc aaccagttca tcgctcaccg cgaggcgcgc ggttttaacg tcatggttta
    10021 ttccccgccg agcggcacga gcaaggaagc cataaaggcc tacattcaga gtttatgggg
    10081 gacggctgac cagccggcct atctgctgat cgtaggcgac accgacggca cctcgacttc
    10141 aaccaacagc accatcccgc actggatagg taccggcagc aaatccgctc cgaccgacct
    10201 gccgtatgta tgcatggact ccgtccccgt gagctggtac ccggattttt accttgggcg
    10261 cttctccgtg cgcgacctca gcatgctcaa cgccgtagtc gctaagacga tcgcggtcga
    10321 aagcggcggc ttcagcgatc ctggatacgt gcgccgggcg gccctgctgg ctaacgacga
    10381 ctccactacg cattccatag aattgcacga ttggatcatc agcacctatc tcgagccggc
    10441 cgggttcacg ccgcatcgca tttacgctgc ctacggcggc agcacggccg acgtcagcgc
    10501 cgctgtaaat gccggcgtat tgttcaccgt ctatttcggg cacagcgggt ccagcggttg
    10561 gtggtcgccg gccttttatc aagccgacgt tcaaaacctg acgaacaacg gcctctatgg
    10621 tctaaccatg ggttggtcct gcagctccgc ctattaccct acctcagagt gcttcggcga
    10681 aacctggttg cgcgtcgcga accgtggttc ggccgcctac ctgtcttctg caacatccgt
    10741 gtggtggggc tcggactcct cctgggaatc gtcgcggcgc atggaacgct acatcttcca
    10801 gtccttcttc gccgacaaca tctgggaagt cggcccggct tggcacgcgg ccctgggacg
    10861 catgtacaac gaccctgaat tcggcccggc gcatgatcac acgcacaaca tcttcgagat
    10921 gatggtcctg ttgggcgacc ctgccctgcg gctgccgttc cgcgcgctcg atatctctct
    10981 gatggagggc gtgcccgaat tcatccaccc cgatgagcca accatattca cagtgaacat
    11041 cggctcggcc gccgagcaat acgtagccga cacggccctg ctgcactact cactcaacgg
    11101 tgaagacttc cagacggcgc cgctggttca cctgggcgga agcgagttcc tggccagcct
    11161 gcccgcgccc ggctgcggca gcacgccccg cttttacttc agcgcgcagg gcgatcaggg
    11221 ctcgaccgtg tacctgccgt atctggcgcc ggcctcctgt ttcaccgccg aggttgccgc
    11281 aatcacaacc ctgctcgatg acgatttcga ggccgatacg ggctggactg tgtggagcga
    11341 cccggccatg gtgagtggaa tgtggcagcg gggcatcccg gtgagcccgg tgccggaagg
    11401 cgcgcccaag gccgattttg acggctcggg cagttgtttc cttacggaca atcgcagccc
    11461 caactacgac gtggacctgg gaccgaccat cctgttgtcg ccgatcatca acttggccgg
    11521 ccggcccgac ccgtacgtgc gttatagccg gtggatgtat tgcgatgacg taattgcgcc
    11581 ggcccgggac tacctcttgg tgcaatgctc gtctgacggc ggcgcaaact ggactacggt
    11641 cgagcaggcc tgggacgacg gggcatgggt ccagcgcgag ttccgtgtgc gcgactttgt
    11701 gacgccgacc gcccagttcc aactgcggtt ttccgtcgcg gacaacccga acaactccgt
    11761 gaccgaagcc ggcgtggacg cagtctgggt gtacgatcgg ggctgcgggg acgtgccccc
    11821 cgttctgggt gacttaaatt gcgacggcaa tgtcaacgtt ttcgacatcg atccgttcgt
    11881 gctggcgctg acggaccccg cggggtacgc actggcgttc ccggcctgcg acgcaatgaa
    11941 cggcgacgcc aaccaggatg gcgaggtcaa cgtcttcgat atcgatccgt ttgtggcgct
    12001 cctgacctcg tcggcccctt gacggcctga gacggcgcac tgccaaaccg tcggcataag
    12061 tcactgaagc gttgcggccc ggctttttcc gggccgccgc tcaaattgca gagcgctcca
    12121 gcaccttcgc gaccagctca accggcaaac cgaccacgtt cggaaaactg ccctcgatcc
    12181 gctcgatcag ccgatcgccg cagtcttgta tgccgtacgc cccggccttg ccgcgccact
    12241 ctccgctttt caggtacgct tcgatttcgg ccgcgtcgtc gcgcatccag accgcagtta
    12301 cgtcggctcc aaacagccgc cgcggccggc cgtttatccg caccagtgaa acgccggtaa
    12361 tcacgtcgca gcgccggccg gcttgcagga ggagcatgcg ccgtgcgtca gccagatcgc
    12421 gtggtttgcc gagcaggtgc tgcccgcagg ttacgatcgt gtccgctccc agcacgagcc
    12481 ggccgggatt ggcttcggcc actgcccggg ctttaaagta ggccagtgct tccgcccagc
    12541 cgcgcgggtt caccgactcg ggctttgcgt ccggctccgc cagcgggcag gcgcgaattt
    12601 cgaacgcagg cacgatcgtt cgcaagagtt cgcgccgccg tggactggcg gacgccaaga
    12661 tgagagctgg cttgggcatt aatcgacctt gcctgcgttg acctcgctcg gcacgcgcca
    12721 ttagataaac cagaatgccg aaggtcaaag ccacccgcac gattcgcacc agacgctctc
    12781 acgcgccgga caagcggcgc agcgccactg cccaccgctt tggcgcgcat acctcgatcg
    12841 ccggcggcat gcatcacgcc cttgaaatcg ggctgcgcct gggctgcgat accgtccagg
    12901 tattcgtcaa aaaccagcgc caatggcggg cggccccact acgcgcggat gacctgcgct
    12961 gctggaacgc ccttctggat actcccggct tcggcccggt catcgcccac gcgac
//
LOCUS       DAOSYS010000085        12997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4041_length_12997_cov_5.299567, whole genome shotgun sequence.
ACCESSION   DAOSYS010000085 DAOSYS010000000
VERSION     DAOSYS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4041_length_12997_cov_5.299567"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2113)
                     /locus_tag="PLU99_07010"
     CDS             complement(<1..2113)
                     /locus_tag="PLU99_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO16843.1"
                     /translation="MQTGKPPLLDLLPHQVLIFDGAMGTNIHAFNLPLSDYRGLENCS
                     EILIETRPDVIEQIHRSFLEQGCDIVETNTFGANRGVLNEYGLAARTYELNVQAAQLA
                     QRVASAYSTPKQPRYVAGSVGPGTRLVSLRQTNYDELLAGYLEQMRGLLDGGVDLIMI
                     ETCQDILQTKAAIQAARLAFERCSRRVPLAVLITIETSNTMLIGTELPAALAAIEAFP
                     EVDIFGLNCATGPQEMSAHVRFLAGHSSRHLAVLPNAGIPSLLNGQPRFPLTPAELAR
                     WLREFVEADGVKLVGGCCGTTPEHLAAVVQAIRGPGAAKHHGSSPGHETARPSNTKPQ
                     REPAVSSLYSATALRQETSCLLIGERCNTNGSRKFKRLLAEENLDGLVEMALAQVREG
                     AHVLDVCVDFVGRDGVTDMQRVVDRFAREVAVPLMLDSTQPEVIEAGLKLAGGKCIVN
                     SVHLEDGEEKLARIAGLLRQYGAAVVALTIDEDPQQAMAKTAARKLEVALRIHELLTR
                     KYGIREEDILFDCLTFPVTTGNAADRRLALETLDGVAAIRRALPNCQLSLGISNVSFG
                     LSPAARIVLNSVFLSEARARGLTAAIVHAGGILPQNQIGPERWQAALDLIYDRSQDGA
                     APLERFISLFADGEQLAQTVRISELPLEERIKQRIIEGDRLGMEDDLNQALRRFSAID
                     IINNLLLEGMKTVGELFGSGQM"
     gene            2375..3730
                     /locus_tag="PLU99_07015"
     CDS             2375..3730
                     /locus_tag="PLU99_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOO16844.1"
                     /translation="MADVSSELAAFFETGEVNPETIERIADLAHATFAARERFGELLT
                     EYQRRVEQGQGEAIKLAAGRLILGQFDAAREWFGKTPDGRLRRFYAAKAAIGQRRPEE
                     ALAELRQAAQKGWDEFEVDMSAAAIHVHEGRLPTAQQLLDKHAQAGQDRAEWYFLKAL
                     IEEHEDRREAAVEDYERALTLDPDHERAMFRCARLYDIQGNDEQAIELYQRLSLQPRA
                     YVNSLINLSVIYEDLGRYDEALECLQRVLKAWPNHARARLFYKDVLSCRQMVVAEAGD
                     EQVEPRNRLLDAAISEFELSVRARNCLKKMNIRTIGDLLRLNEAELTSYKNFGETSLA
                     EIKALLAKRGLRLGQAAEEAAESGAAAAPSVAAPPPTPAVNVPPGREALLSKPVSELE
                     LSVRARRCLQRLNVVTLGDLIQLTEADLLATRNFGVTSLNEVKGRLAEFGLSLAPKRS
                     E"
     gene            3768..4874
                     /gene="tgt"
                     /locus_tag="PLU99_07020"
     CDS             3768..4874
                     /gene="tgt"
                     /locus_tag="PLU99_07020"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOO16845.1"
                     /translation="MTTAGKARAGVIRTPHGQIHTPAFMPVGTQATIKGLTPAQICET
                     GTQILLANTYHLLLRPGAEVVEQLGGLHRFMGWNGPILTDSGGFQVFSLARLRRLDDE
                     GVEFQSHVDGALVRLTPEVSIAVQNALGADIIMAFDECPPLPAEPHVLYAAVRRTLAW
                     AQRSRAAHQRADQALYGIVQGGLDQSLRQECLGRLVEIGFDGYALGGLSVGEPPPEMW
                     RFLDDFAWRLPSDRPRYLMGVGTPRDLLEAVAAGIDQFDCVLPTRNGRKGYAFTSRGV
                     VRLRNSVHRLSAEPLDPACDCQTCRNFSRGYLRHLFMSGEILGGTLVSLHNIRFYQNV
                     MAQTRAAIADGTFDAFRRDFLTSPAAEANRELDD"
     gene            4867..5214
                     /gene="yajC"
                     /locus_tag="PLU99_07025"
     CDS             4867..5214
                     /gene="yajC"
                     /locus_tag="PLU99_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOO16846.1"
                     /translation="MIDLLLTLAQTTQPTGAPPSGTEMLLRQFLPLILIIGVFWWWMS
                     RSRRREQQRYQDMLRNLKRNDRVQTIGGIIGTVVDTRDDEVVLKVDETNNVKMRFVRS
                     AIKDVMRESTPSS"
     gene            5278..6204
                     /locus_tag="PLU99_07030"
     CDS             5278..6204
                     /locus_tag="PLU99_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO16847.1"
                     /translation="MASLAQYLDPEVIQQVSRLDLRARFIVEGFLAGLHASPLQGFSV
                     EFSEHRKYVQGDDVRAIDWNVFGRTDRLYIRKYNAETHLECFLLVDASGSMGYVGGLG
                     PIGLPSPQPAAKLMYAMHLAAALGYLVTHQQDAVGLGIIGAQGLKQFLPARSRRQHLV
                     QLLSALAAVVPQGQTGLAEGISQALAQIRHRGLVVVLSDLLTDQEKTLEALHHVRFRG
                     HDLIVMHILDAAEVRFPFEGSLRLEDPETGESLVADAAAVRPRYVEAVEAWRSELRDR
                     LAAARADYVPLDTSMPFDKALVEFLTQRGRRS"
     gene            complement(6292..7293)
                     /locus_tag="PLU99_07035"
     CDS             complement(6292..7293)
                     /locus_tag="PLU99_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16848.1"
                     /translation="MSRWKTAVGTHLLLSAAAAFGAPDGLPASYCSISCDSPAAFSRG
                     ASSGQLYISWDRGPGDRRYVIDNFAGFAGSIHSIKWWGNSTILWDGPDCDFEQPAPFV
                     IKFYHSSGDGPDYQNPSAVYDPVYATYADTGYTWIGMPQLGQIDCYTVLLPTGYTPGD
                     GVHWISIASKTADCDFVWAPGTADGPAPSQWLCDNGICGPDPSQGHRSFCLYTADSAG
                     ACCNEVTGACTEGPSEPDCLTLGGTFYENQSCDELPVPSTCGPCGPTADANCDGAVNT
                     FDIDAFVVALTNPGLWRALQPCDLLCAADCNRDGSVNTFDIDAFVLCLTGDCPSQPE"
     gene            complement(7419..7501)
                     /locus_tag="PLU99_07040"
     tRNA            complement(7419..7501)
                     /locus_tag="PLU99_07040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7465..7467),aa:Leu,seq:tag)
     gene            complement(7599..8678)
                     /locus_tag="PLU99_07045"
     CDS             complement(7599..8678)
                     /locus_tag="PLU99_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO16849.1"
                     /translation="MKLRLTLGVLVVLIAAGLMLWFQMEVLLLGFTGVLLAIFLFTVS
                     DWVARWTHVWYWLALLVVCLVLLAATAGGVWLLADQIAPQIRTLREQLPEAWQTVKSY
                     MEQNPAGAWVLDQAGAGVETLQDGTVQQREVISKGLGYLSSFGLVLTHALVVLFVGLF
                     GAITPSLYVRGMLRLVPMRRRQRAREVLQALRRLLWWWIVGQLIAMVFIGVATSVALW
                     VMGIPLALALGVIAAVLNFIPNFGPILATIPAGLVALMQGPAALVWVIIIYIIIQAVQ
                     NHVVMPVTMQAAVELPASILMLAQVFMWYWAGLLGLALAPALAAVVIKLVQMLYLEDY
                     LGDPMRRDWSFWPEGAEESPEKGGG"
     gene            complement(9164..10033)
                     /locus_tag="PLU99_07050"
     CDS             complement(9164..10033)
                     /locus_tag="PLU99_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO16850.1"
                     /translation="MDKLPDNVVLSVRDLVVRFESKGNGPVTVLEGISFDVYRSETLV
                     IMGGSGSGKSTILNCLIGEIEPEAGRIIYNLPELGTVDLAHTDEETRDALRKRFGILF
                     QSGALFSSMTVAENVALPLREHSHVPESVIDIVVAIKLQQVHMLPHRDKLPSQLSGGQ
                     RKRAALARATALDPELLFYDEPSSGLDPITANSIDELILDLTHKLKVTSVVVTHDMAS
                     AMRIADRAIMIKGGRILRMGPRAEFEALRDASPDSLQSPEDRILHQFLIGSSEALREE
                     GELGEFEKLIVGK"
     gene            complement(10073..10336)
                     /locus_tag="PLU99_07055"
     CDS             complement(10073..10336)
                     /locus_tag="PLU99_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO16851.1"
                     /translation="MPRYEYQAKDPQQACDVCRQRFEVYQSMAEQPLTACPQCGRPVT
                     RMISLCAVGTQPSERAMLSDKNLKEKGFTKLVNEGHGRFRRTV"
     gene            10644..12164
                     /locus_tag="PLU99_07060"
     CDS             10644..12164
                     /locus_tag="PLU99_07060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO16852.1"
                     /translation="MSRPQRDSSKHAHKPTDVVETTPPRRVIRRFELSGLLTGLAIFV
                     VALGVRLAYLYDSSDSPTFRTPIVDAKNYDNLARLLSPHIFADGPADEKLSLADRLLL
                     FWQPFLYPYTLGALYRIAELRGQDPLLFAKIVQLVVGAATCVLVYALGRRLFGRGTGI
                     IAALMTAFCGPLIYYEADLVAAGWAAFWSAALVLLAVKAGRDGSIVAAWLAGAGGGLA
                     VLMRPTFLPFLLALLVWLIVRCIRDKRGVVGSLVRVVPAVLLFGLVVYPVAKKAHNFE
                     LIQKYTFLPVSGGINFYIGNNENVCETLNTRPGMTKWAKLAEPSGAAGDTNTLERSQL
                     LGRRAMEFVKTHPFKFVGGLFDKVLRFTSGREMPRNEDVYVFRRWSPVLSVLLWKAGP
                     FGFPWGVLFPLALVGLVWRRKDVPGPLALYLVLYPLSIILIFVSGRYRVETVPVFAVP
                     AAACVWGMIEAARARKWLLVGTARLRGGAGCADQLARALLRRRGELRGRDVRLAGR"
     gene            12124..12834
                     /locus_tag="PLU99_07065"
     CDS             12124..12834
                     /locus_tag="PLU99_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16853.1"
                     /translation="MNYEAEMYVWLGDDKRLQAKPLLKIPYDASEEVVKALQAEADRL
                     HNEALAFYEKAVTLAPDSVVARVHLGYLLKNMRRYQEALQHLDVVLQQEPDFLAYFYR
                     GDVYSRMRDQERALRDFTSCVEAEPRFKPGLLARSAIYQGQGRVEEAKADCRRVLELC
                     AQVTEYQEARRALKQQIEQVRARAKAEGRALTQSEIEYIKPREEMIGILNYRIENHPD
                     RDAAHSASARLRQLEQQS"
BASE COUNT         2368 a         4143 c         4112 g         2374 t
ORIGIN      
        1 gcatctggcc gctgccgaac agctcaccga cggtcttcat gccttccagc agcaggttgt
       61 tgatgatgtc gatcgccgaa aagcgccgca gcgcttgatt caggtcgtcc tccattccca
      121 gccggtcgcc ctcgatgatc ctttgcttga tgcgctcttc cagcggcaac tcggagatcc
      181 tgaccgtctg agcgagctgc tcgccatcgg cgaagaggct gatgaagcgt tccagcggcg
      241 cggcgccatc ctgcgagcgg tcgtaaatca ggtccaacgc ggcctgccaa cgctccggcc
      301 cgatctggtt ttgcggcaga attccaccgg cgtgcacgat ggccgccgtc agcccgcgcg
      361 ccctggcctc ggacaggaag actgagttca ggacaatccg tgccgccggg gacaggccga
      421 aactgacatt gctgattccc aggctgagct ggcaattcgg cagcgcccgc ctgatggccg
      481 ccacgccgtc aagcgtctcc agggccagcc ggcgatcggc ggcgtttccc gtggtgacgg
      541 gaaaggtgag gcagtcgaag aggatgtcct cttcgcggat tccgtatttg cgggtcaaaa
      601 gctcgtggat gcggagtgca acttcgagct tgcgggccgc agtcttggcc atggcctgtt
      661 gcgggtcttc gtcgatcgtc agggcgacga ccgccgcgcc gtactgccgc agcagccccg
      721 cgatgcgcgc cagcttctcc tcaccatcct caagatgcac cgagttcacg atgcacttgc
      781 cgccggcgag cttcagaccg gcttcgatga cctccggctg cgtgctgtcg agcatgagcg
      841 gaacggccac ctcgcgcgca aagcggtcga cgacccgctg catgtcagta acgccgtccc
      901 gcccaacgaa gtccacgcag acgtccagca cgtgcgcccc ctcacgcacc tgtgcgagcg
      961 ccatttcgac caggccgtcc agattctcct cggccagcag acgcttgaat ttccgcgagc
     1021 cgtttgtgtt gcagcgttcg ccgatcagca gacaactcgt ttcctggcgc agggccgtgg
     1081 cggaatacag gctgctgacg gccggctctc gctgcggttt ggtgttcgag ggccgggcgg
     1141 tttcgtgtcc cgggctggag ccgtggtgct tcgcggcgcc cggacctcgg atagcttgga
     1201 ccacggcggc taggtgctcg ggcgtggttc cacaacagcc accaaccagc ttcacgccgt
     1261 cggcctcaac aaactcgcgc agccagcgtg ccagctccgc gggcgtcaag ggaaaacgcg
     1321 gctgaccatt caagaggctt ggtattccgg cgttgggcag aacggcgagg tggcgcgagc
     1381 tatggcccgc caggaaacgc acgtgcgcgc tcatttcctg tggtccggtc gcgcaattca
     1441 ggccgaagat gtccacctcc ggaaaggcct cgatggccgc cagggctgcc ggcaactcgg
     1501 tcccgatcag catagtgttg ctggtctcga tcgtgatcag tactgccagg ggcacgcggc
     1561 gcgaacagcg ttcgaacgcc aggcgggccg cctgaatggc ggccttggtt tgcaggatat
     1621 cctggcacgt ttcaatcatg atgagatcga cgccgccgtc gagcagcccg cgcatctgct
     1681 ccaggtaacc ggccagcagc tcgtcatagt tcgtctgccg cagcgaaacc aggcgcgtcc
     1741 ccgggccgac cgaacccgct acgtagcgcg gctgcttcgg cgtgctgtag gccgaggcga
     1801 cgcgctgcgc cagttgggcg gcctgcacgt tcagctcgta agtccgcgcg gccaggccgt
     1861 attcgttcag cacgccgcga ttggcgccga aggtattcgt ctcaactatg tcgcagcctt
     1921 gctccagaaa ggagcgatgg atctgctcga tcacatccgg ccgcgtttcg ataagaatct
     1981 ctgaacaatt ctccaggccg cggtaatcgc taaggggcag gttgaaagcg tggatattgg
     2041 tgcccatggc gccgtcgaag atgagcacct ggtgaggcag caagtcgaga agcggcggtt
     2101 tgcctgtttg cacgcgggat actccaggcc ggctgcctga cggttcggtc ggggctcacg
     2161 cacgcctacc ggcgccgggc cgggacgcca cagcggcgtc cggccggatc cgtgacggcc
     2221 gtcctcgcaa agcgggtcga ctgcggcgtg cttgggtcgc ttgcagcgct gcggtcctta
     2281 caacgggctt gtcagccggc gaacgtttga tagtatagct tgtttgacgt ttatcggctc
     2341 taccattaca ggttggtttc ggagcactgc gcccatggcg gatgtcagta gcgagctcgc
     2401 ggcttttttt gagacgggtg aagttaatcc cgagacgatt gagcgtattg cggacctggc
     2461 gcacgcgact ttcgcggcgc gcgaacggtt cggcgaactc ctgaccgagt accaacggcg
     2521 cgtcgagcag gggcagggtg aagcaatcaa gctagccgcc ggccggctga tactggggca
     2581 gtttgacgcc gcgcgtgagt ggttcggcaa gacgccggac gggcggctgc gccgctttta
     2641 cgccgcaaag gcggccattg ggcaacggcg tccggaggaa gctttggccg agctgcggca
     2701 agcggcccaa aaaggatggg acgagttcga ggtggacatg tccgcggccg ccattcacgt
     2761 gcacgaagga cgcttgccaa ccgcgcagca actgctcgac aagcacgcgc aggccggtca
     2821 ggaccgcgcc gaatggtact tcctcaaggc cctgatcgag gaacacgaag accggcgcga
     2881 agccgcggtc gaagactatg aaagagcact gacgctcgac ccggaccacg agcgggccat
     2941 gttccgctgc gcccggctgt acgacatcca aggtaacgac gaacaggcga ttgagctgta
     3001 tcaacgcttg tcgctgcagc cgcgggcata cgtcaactct cttattaatc tctcggtcat
     3061 ctacgaagac ctcgggcgat acgacgaggc gctggaatgc ctgcagcgcg tcctgaaagc
     3121 ctggccgaac cacgcccggg cccggctgtt ctacaaggac gtcctgagct gccggcaaat
     3181 ggtcgtggct gaggccggcg acgagcaggt cgagccgcgc aaccgcttgc tggatgcggc
     3241 catctccgaa ttcgagctgt cggtgcgggc ccgcaactgc ctcaagaaaa tgaacatccg
     3301 cacgatcggc gatttgctgc ggctgaacga ggccgagctg accagctaca agaactttgg
     3361 ggaaacctcg ctggccgaga tcaaggccct gctggccaag cgcggcctgc ggctgggtca
     3421 ggcggctgag gaggctgcgg aaagcggagc ggctgcggct ccctcggtag cagcgccgcc
     3481 gccgacgccg gcggtgaacg tgccgccggg acgcgaagcg ctgctctcta agccggtcag
     3541 tgagctcgag ctttcggtcc gcgcccgccg ctgtttgcag cggctgaacg tggtgacgtt
     3601 gggcgatttg attcaactta ctgaagcgga cttgctggcc acgcgcaatt tcggtgtcac
     3661 ttcgttgaac gaagtgaagg gccggctggc cgagttcggg ctcagtctcg caccgaagcg
     3721 gtctgagtag cggtcccggg gtgtcgggcg gcttctcgtt tcagcttatc acgaccgcgg
     3781 gaaaggcgcg tgccggagtg atccgcactc cgcacgggca gattcacacg cccgcgttca
     3841 tgccggtcgg gacgcaggcc accatcaagg ggcttacgcc cgcacagatt tgcgaaaccg
     3901 gcacgcagat actactggcc aacacgtacc acctgctgct gcggcctgga gcggaggtcg
     3961 ttgagcagct tggcgggctg catcgcttca tgggctggaa tggcccgatt ctgacggaca
     4021 gcggcgggtt tcaggttttc tcgctggccc gcctgcgccg gctggacgac gaaggcgtcg
     4081 agtttcaatc gcacgtggat ggagcgctcg tgcgcctcac gccggaagtg tcgatcgcgg
     4141 tccagaacgc gctcggggcg gacatcatca tggccttcga cgagtgccct ccgttgccgg
     4201 ccgagccgca cgtcctctac gccgcggttc ggcgaacgct ggcctgggcc caacgcagcc
     4261 gcgccgccca ccagcgcgct gatcaagcgc tttacggcat cgtgcaaggc gggctcgacc
     4321 agagcttgcg gcaggaatgc ctgggccggc tggtcgagat cgggttcgac ggttacgcgc
     4381 tgggcggcct ttccgtgggc gagccgccgc cggagatgtg gcggtttctg gacgacttcg
     4441 cgtggcgcct gccgagcgac cgcccgcgct atctcatggg cgtcggcacg ccgcgcgatc
     4501 tgctcgaagc cgtcgccgcc ggaatcgacc agttcgactg cgtgctgccc acgcgcaacg
     4561 gccgcaaggg ctacgcgttc accagccgcg gggtggtgcg actgcggaat tcggtgcacc
     4621 ggctttcggc cgagccgctg gatccggctt gtgactgcca gacgtgccgc aatttttcgc
     4681 gcggctacct gcggcacctg tttatgtcgg gcgagatact tggcgggacg ctagtatcac
     4741 tacacaatat tcgtttttat cagaacgtga tggcgcaaac gcgggccgcc atcgcggacg
     4801 gcacgttcga tgcgttccgc cgcgacttct tgaccagccc cgcggctgaa gcgaacagag
     4861 aactcgatga ttgacctact gctaacgttg gcccagacga cacagccgac aggcgcccca
     4921 ccctcgggca cggaaatgtt gttgcgccag ttcctgccgc taatcttgat tattggtgtc
     4981 ttctggtggt ggatgtcgcg gtcgcggcgt cgcgagcagc agcgctacca ggatatgctt
     5041 cggaatctca agcgcaatga ccgcgtccag accatcggcg ggatcatcgg gacggtggtc
     5101 gacacccgcg atgacgaggt ggtgctgaag gttgacgaga cgaacaacgt gaaaatgcgt
     5161 ttcgtccgca gcgccatcaa ggacgtgatg cgcgaatcca cgccctccag ctaatacggc
     5221 ggacgtgccg tgccagagcc gcgttgcgcc ggtctttctc cggtgtagaa gtgactgatg
     5281 gcgtcgctgg cccagtatct tgatcccgag gttatccagc aggtcagccg gctggacttg
     5341 cgggcgcgtt tcatcgttga aggctttctg gccggcctgc acgcttctcc actgcaaggc
     5401 ttttccgtcg agttcagtga gcaccgcaag tacgttcagg gtgacgacgt gcgggccatc
     5461 gactggaacg tcttcggccg cacagaccgg ctctacattc gcaagtacaa cgccgaaaca
     5521 cacctggagt gcttcctgct ggtggacgcc tccggcagca tgggctacgt gggcgggctc
     5581 gggccgattg gcctgccctc tccgcagccg gccgcgaaac taatgtacgc catgcacctg
     5641 gcggccgcct tgggctatct cgtcacgcac cagcaggacg ccgttgggtt gggcataatc
     5701 ggggcgcagg ggctcaagca gtttctcccg gcccgctcgc ggcgccagca tctggtgcaa
     5761 ctcctaagcg ctctggccgc agtggtcccg caggggcaga cgggcctggc ggagggcatc
     5821 agccaggcgc tggcccagat tcgccatcgc ggcctggtgg tcgtactcag tgatctgctc
     5881 accgatcagg aaaagacgct cgaggcgctg caccatgtgc gcttccgcgg ccatgacctc
     5941 atcgttatgc atatcctgga cgcggccgaa gtccgctttc cgttcgaagg cagcctgcgc
     6001 ctggaagacc cggaaaccgg cgagagcctg gtagccgacg ccgccgcagt ccgcccgcgc
     6061 tacgtggagg cggtcgaggc gtggcgcagt gaactccgcg accgcctggc ggcggcccgg
     6121 gcggactacg tgccactcga cacgtcgatg ccgttcgata aggcgctagt ggaattcctg
     6181 acccagcgcg gccggcggag ctaagcgtca gggtgataga gtgatagatg atagggcgat
     6241 aagggcgcga tccgcggacc cactttatca ctctatcact ttatcacttt atcactctgg
     6301 ttggctgggg cagtcgcccg tcaggcacag aacgaacgca tcgatgtcaa acgtattcac
     6361 gctaccgtcg cgattgcagt ccgcggcgca gaggaggtcg cagggttgta gcgcccgcca
     6421 taagcccggg ttggtaaggg ccaccacgaa cgcgtcaatg tcaaacgtgt tcacggcgcc
     6481 gtcgcagttg gcgtcggctg tcgggccgca gggcccgcaa gtgctcggca caggcagctc
     6541 atcacagctc tggttttcgt agaaagtgcc accgagagtc aggcagtcgg gttcgctcgg
     6601 gccttccgta cacgcgccgg taacctcgtt gcagcaggcg ccggcgctgt cggcggtata
     6661 cagacagaag ctgcgatggc cctggctcgg gtcaggtcca caaatgccat tgtcacagag
     6721 ccattgactg ggggccgggc cgtcagccgt gcccggggcc cacacaaaat cacagtcggc
     6781 cgtcttgctg gcgatcgaga tccagtgcac accgtcgccg ggagtgtagc cggtgggcag
     6841 caacaccgtg tagcaatcga tctgcccgag ttgtggcata ccaatccagg tatagcccgt
     6901 gtccgcatat gtcgcgtaga ccgggtcgta cacggctgag ggattttggt aatccggtcc
     6961 gtcgccgctg gagtgataga acttgatcac gaacggcgcc ggctgctcaa agtcgcaatc
     7021 cgggccgtcc cagaggatgg tggagttgcc ccaccacttg atactgtgga tgctgcccgc
     7081 aaagcccgcg aagttgtcaa tgacgtaacg ccggtcaccc gggccgcggt cccaggatat
     7141 gtaaagctgc ccgctcgacg cgccgcgact gaatgcggcg ggcgagtcgc aggagatgga
     7201 acagtagctc gccggcaacc cgtcaggcgc accaaacgcc gcggctgcgc tcagcagcag
     7261 gtgtgtgcca actgctgttt tccagcgaga catttcccat cagagtaaac actcatcggg
     7321 caaggaacaa gcgggttgat actgatgtcg ccacagcgta tgcgacaaac gggctccgct
     7381 tttcttggcc caatcaccga aatgcttaaa cgactaattg cgagaggggg gactcgaacc
     7441 cccacggggt ttacccacca gaacctaaat ctggcgcgtc tgccaattcc gccactctcg
     7501 cggacacgct tccatgatac cgcaggaacg cctcccgcca agccccgccg tagcgccgcc
     7561 cgggaccgag cccggcacgc acctgcgacc caaggccctc agcccccacc cttttccggg
     7621 ctctcctccg caccctccgg ccaaaagctc caatcccgcc gcatggggtc gcccaagtaa
     7681 tcctccaggt acagcatctg caccaacttg atcaccaccg ccgccagcgc cggcgccaag
     7741 gccagcccca gcagccccgc ccaataccac ataaacacct gcgccaacat cagaatactg
     7801 gccggcaact cgaccgccgc ctgcatcgtt accggcatga ccacgtggtt ctgcacggcc
     7861 tgaatgatga tgtatatgat gatcacccag accagcgccg ccggcccctg catcaaggcc
     7921 accagcccag ccgggatagt cgccaaaatc gggccaaaat tggggataaa gttcaacacc
     7981 gccgcgatta ctcccaacgc cagcgccagc gggataccca tcacccacag cgccaccgat
     8041 gtcgccacgc cgatgaagac catcgcgatc aactggccga cgatccacca ccataacaac
     8101 cggcgcaacg cttgcagcac ctcgcgtgcc ctttgccgcc gtctcatcgg caccagccgc
     8161 agcatgccgc gcacgtacag cgacggcgtg atggcgccga acagcccgac aaaaagaacc
     8221 accagcgcgt gtgtcagcac cagtccgaac gacgacaggt aacccagccc tttggagatg
     8281 acttcgcgtt gctgtaccgt tccgtcctgc aaggtttcca cgcctgcgcc agcctggtcg
     8341 agcacccacg caccggccgg gttttgctcc atgtatgatt tgaccgtttg ccatgcctcg
     8401 ggcaattgct ctcgcagcgt tcggatctgc ggcgcaatct ggtctgcgag cagccacacc
     8461 ccgcctgccg tcgctgccag caacaccagg cacacgacca acagcgcaag ccagtaccac
     8521 acgtgggtcc agcgcgccac ccagtcgctc accgtgaaca agaagatggc cagcagcacg
     8581 cccgtaaatc cgagcagcag tacctccatc tggaaccaga gcatcagccc ggcggcgatc
     8641 aacaccacaa gaacgccgag cgtcaggcgc aatttcaacc agaagatcca cagctcggtt
     8701 cgcgccggcc atagcggcgc ttgcaccgct atcggcggcg cagaaggcgg tggctgagcc
     8761 ggcggcggtc tgggcggcgc gacctccgcc ggtgcgctcg gtggttcggg ttctggaggc
     8821 ggcggattgg gaggcgactg gctcacgctt ccttccggac tggcgttgtc ggtaatcccg
     8881 cgattcgcgc gtcgacgccg atcgcgcagc accgaatctt gctttgacgc tctgaacgaa
     8941 atgtgaattc tgaatgagcg ccttcacatg gctttgcctt taaggcgagt gtcgcggtcc
     9001 actacattgc agccgcgcag ccgatcacgc cgatttccgg agcatgggtg ccagcgtggg
     9061 tgccatgcat gcccctgcgt gcggcactcc agcacgctcc tggcggtacg ccaccacatt
     9121 ccgaggcgac acgccccggc tctgctctcc cttggcgagt tggttacttt cccacgatca
     9181 gcttctcgaa ctcgcccagc tcaccctctt cccgcagggc ctctgacgac ccgatcagga
     9241 actggtgcaa aatgcgatct tctgggctct gaagcgagtc cggggaagcg tcgcgcaggg
     9301 cctcgaattc cgcacgcggc cccatgcgca gaattcgccc acccttgatc atgatggccc
     9361 ggtcggcaat ccgcatagcc gaagccatat catgcgtaac cactacgctc gtcaccttca
     9421 gcttatgagt taggtcgaga atcagctcgt cgattgaatt ggccgtgatt ggatcgaggc
     9481 cggagctcgg ctcgtcatag aacagcagct ccggatcgag ggccgtggcc cgggccaggg
     9541 ccgcccgctt ccgctgcccg ccggaaagct gggacggcag cttgtcgcgg tgcggcagca
     9601 tgtgaacctg ctgcagcttg atcgcaacga cgatatcgat caccgactcc ggcacgtgcg
     9661 agtgctcgcg cagtggtagg gccacgtttt cggcgaccgt catcgagctg aacagggccc
     9721 cggattggaa cagaatgccg aagcgcttgc gtagcgcgtc gcgcgtctct tcatcggtgt
     9781 gggccaggtc caccgtcccc agttcgggca ggttatagat aatgcggccc gcttcgggct
     9841 cgatttcacc gatcaggcaa ttcagaatgg tcgacttccc gctgcccgag ccgcccatga
     9901 tgaccagcgt ctcgctgcgg tacacgtcga agctgattcc ctccagcacc gttaccggcc
     9961 cgtttccctt gctctcgaag cggacgacca ggtcgcgcac cgagaggacc acgttgtccg
    10021 gcagcttgtc caaatcagcg cctttgcgtt gagccgcccg cgccgcgccc cctcaaaccg
    10081 ttctccgaaa ccgtccgtgc ccttcgttga ccagtttcgt gaagcccttt tccttcagat
    10141 tcttgtcgct caacatggcc cgttccgacg gctgcgtgcc gaccgcacac aacgagatca
    10201 tacgcgtcac agggcggccg cactgcgggc aggcggtcag cggttgctcc gccatcgact
    10261 ggtacacctc gaagcgctgc cggcaaacgt cacatgcctg ctgcggatcc tttgcttgat
    10321 attcatatcg tggcatatcg tcattatata agagcggcgc cggctgcctg tcgcttcaaa
    10381 atctgagcag ccaatcgtgg atagtcctat aaaaatcagc cttggggctt agaaagcaga
    10441 tgctcgcaag cccatccgca gtctgataac ctaatggctg ctcatggagc cagaccgtca
    10501 tcgcagcgca agcaacccaa tccgcgcatc tggctaagag ctgatagctg atagctgaca
    10561 gccggccgcc gaaagctgat agctgaaagc ggaaagcggc ttagaatatc gcccggcgtc
    10621 cagggagtga gcgaggagtt tgaatgagca ggccccagag agactcttcc aagcacgcgc
    10681 ataaaccaac cgacgtggtc gaaaccacgc cgccgcgcag ggtaattcgg cgcttcgagt
    10741 tatccggcct gctgacgggt ttggccattt tcgtcgtggc gctgggggta cggctggcgt
    10801 acctgtacga cagctccgac agtccgacct ttcgcacgcc gatagtcgac gccaagaact
    10861 atgacaactt ggcgcgcctc ttgtcgccgc acatcttcgc cgacggcccc gccgacgaga
    10921 agctcagcct ggcagaccgg ttgctcctgt tctggcaacc gttcttatat ccgtacactc
    10981 tgggggccct gtaccgcatt gccgaactgc gcggccagga tcccctgctg tttgcgaaaa
    11041 tagtgcaact tgtggtcggc gccgctacgt gcgtgctggt ctatgcgctg gggcggcgcc
    11101 tgttcggccg cggcacggga atcatcgccg ctctaatgac cgctttctgc ggaccgctga
    11161 tctactacga ggcagacctg gtcgcggcgg gctgggcggc attctggtcg gccgcgctgg
    11221 tcttgctggc ggtgaaagcc gggcgcgacg gctcgattgt ggcggcttgg ctggcgggcg
    11281 ccggcggcgg gttggcggtg ctgatgcgcc cgaccttcct gccatttttg ctcgcgctgc
    11341 tcgtctggtt gatcgtacgc tgcatccggg acaagcgcgg agtcgtgggc tccttggtgc
    11401 gcgtcgtgcc ggcggtgctg ctctttgggc tcgtggttta ccccgtggcc aagaaggcgc
    11461 acaactttga gctgatccag aagtacacgt tcctgccggt ttcgggtgga atcaacttct
    11521 acatcggcaa caacgagaac gtctgcgaga cactgaacac gcgcccgggc atgaccaagt
    11581 gggccaaact ggccgagccg agcggcgccg ccggcgacac caacacgctg gagcgttcgc
    11641 agcttctggg gcgccgggcg atggagttcg taaaaacgca cccgttcaag ttcgtgggcg
    11701 gtctgtttga caaggtgctg cggtttacca gcgggcgcga gatgccccgc aatgaagatg
    11761 tgtatgtctt tcggcgctgg tcgcccgtgc tcagtgtgct gctctggaag gcgggcccat
    11821 tcggcttccc ctggggagtc ttgtttccgc tggcgctcgt cggcctggtg tggcgccgga
    11881 aagacgtgcc cgggccgctg gcactgtacc tggtgctcta cccgctctcc atcattctga
    11941 tcttcgtcag cggccgctat cgcgtggaga ccgtgccggt cttcgctgtt cccgccgcgg
    12001 cttgtgtatg gggcatgatc gaggccgccc gcgcgcggaa atggctgctc gtggggaccg
    12061 ctcggctacg tggcggcgct gggtgtgctg accagcttgc cagggccctt ctgcgaagaa
    12121 gaggtgaact acgaggccga gatgtacgtc tggctgggcg atgacaagcg cctgcaagcc
    12181 aagccgttgc tcaaaatccc gtatgacgcc agcgaggagg ttgtgaaggc cctgcaggcc
    12241 gaggcggatc gcctgcataa tgaagcactg gctttctatg agaaggcagt gacgctcgcg
    12301 cccgactcgg tggtagcgcg cgtccatttg ggatacctgc tcaaaaacat gcgcagatac
    12361 caggaagcgc ttcagcactt ggacgtcgtt ctgcagcagg agccggactt cctggcctac
    12421 ttttaccggg gcgacgttta ctcgcgcatg cgcgaccagg agcgggcgct gcgcgacttc
    12481 accagttgcg tggaagccga gccgcgcttc aagcccggtc tgctggcacg cagcgcgata
    12541 taccaggggc aggggcgggt ggaggaagcg aaggccgact gccgccgcgt gctcgagctg
    12601 tgtgcacagg tgaccgaata ccaggaggcg cgcagggctc tgaagcagca gatcgagcag
    12661 gttcgcgcgc gggccaaagc cgaaggccgc gctctgaccc aaagcgaaat cgaatatatt
    12721 aagccgcgcg aagaaatgat cggtatcttg aattacagaa tcgagaacca cccggaccga
    12781 gatgcggcgc actcggcttc ggcgcggctg cggcagttgg agcagcagtc gtgagtattc
    12841 ggtacccctg agttgcactt gactggagca cagaagtgag tggcaaaagg tcattgtggt
    12901 gctgtgctgc gggctggctg acactgcttt tcgtgacggg cggcccgctg ggggccgcgg
    12961 aagacccgcc accgccggca gccgaggagg ccgcgct
//
LOCUS       DAOSYS010000086        12802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4123_length_12802_cov_5.401271, whole genome shotgun sequence.
ACCESSION   DAOSYS010000086 DAOSYS010000000
VERSION     DAOSYS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_12802_cov_5.401271"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PLU99_07070"
     CDS             complement(<1..452)
                     /locus_tag="PLU99_07070"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOO16854.1"
                     /translation="MTTAASGRNASWIGWAVLEWTARLAVALLFIYTGYTKVMDPATF
                     IKEVRSYELMPLALSNFTALVLPWVEIIAGLLLIASVWRREARILLALMLVVFIVAKA
                     AGHGGDCGCVPTYSFIKPLFEGWMGFVTNAVLLLLLGVETFLERLRRKP"
     gene            complement(449..1075)
                     /locus_tag="PLU99_07075"
     CDS             complement(449..1075)
                     /locus_tag="PLU99_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOO16855.1"
                     /translation="MLRSGVRILVLLAGATLIGVGYGRYRQIPLVPDVQQIKEERDQR
                     AIWVQQTGIDLADFVRHYQAGRLVIDARAPEAFEEGHLDAPLIMNIPAEAAHDCLHRA
                     LSFIGTPIVIYCSSADCESAQELWNDMRSWAFPPETLRVFHPGWVGIREAKLPIACGP
                     DMFQDDPWRQSEEVETSVAEDYADAAAESGDEPQGDDSPGAEEEQEAP"
     gene            complement(1122..2279)
                     /locus_tag="PLU99_07080"
     CDS             complement(1122..2279)
                     /locus_tag="PLU99_07080"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOO16856.1"
                     /translation="MNTLIAVLVNPRSAVLMLALGAVLSALTCENALAQPAGPAAEGA
                     AGVPESADSSGVPRLELSPTALNFGEVWQGERAAGDFTVRNVGTAPLTLTLKSSCGCT
                     VASKPRSPLEPGESDTFRITYDTLKRKGKANQTVTLTTNDPDQPNVRIAVTGEVKPLY
                     STSPAEGLVFDRLTSDSQVTQTLRIESKYEGPLHLKLRPDQAFGPFTIELKEVEPGVA
                     YELVATTKPPLTDDFARTTVVIETDVERSPEIHIPVHARVLQDVSIRPKVLRAQRMLI
                     TPKEEEITVTYRADKPIRITEVRPSTPSISFELLSPRPAREGSDWTAQPIRVILPPGD
                     QLPPGDQFITVLTDSAEPRFQELRVVVRDVQPGTPAASPAAESSENDGAER"
     gene            complement(2321..3418)
                     /locus_tag="PLU99_07085"
     CDS             complement(2321..3418)
                     /locus_tag="PLU99_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOO16857.1"
                     /translation="MPFLCFLRPVIALLVATASEPPAKPAETPDAPANGPRLKLSQDS
                     WNFGTARHLEHLETTVQLSNTGTAELRISNVSTSCGCTAAQPEKRVLLPGESTPLKIT
                     FNTRGKNGPTSANVWIESNDTSEPKKALRVEGKVKRAVKIEPEFAVFRLLSPDELMST
                     RVRVVNTTPQPMQLKLQDYASGKFTAELNEIRAGQEYEIVITTRPPITQRRMADMLHV
                     TTGLEDEPRLEISTQVLQIDRVNFLQPALLVIGERGETLEATGEIEYFGNDPAFRITK
                     AECDDPHAIVRLGAPFAPEANRAGRRPTSIVKFVAEFPPQTVFPPGGLPIRVHTTDPE
                     YPTLTIYATTDTVEYRDLSARARGRSQRKTP"
     gene            3875..4807
                     /locus_tag="PLU99_07090"
     CDS             3875..4807
                     /locus_tag="PLU99_07090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOO16858.1"
                     /translation="MNGRLNRLWAGFLLLVLAVPAWAIKLGDPAPPIQIAQWIKGEPV
                     DLAAGKGKNVYVLEFWATWCQPCRESIPHLTELQKKYKDQGLIVIGITDENNPSAVPA
                     FVAKMGDKMNYTVAYDDNHKTNAAYIQAFGLRGIPHAFVIDREGRIAWSGHPKFGLEQ
                     AVEQIITGKYSLAAARTREETLPLLTRYYELASTGGDETELKSLGRQIVDKVSGDAQI
                     LAMFSFDILHKPEFKYRDLELALDASKRACELAGERADVVAVYARALWDNGKKAEAVE
                     SQRRAVALAQDNPRIRAELEKALAAYEQQLKAQP"
     gene            4899..7847
                     /locus_tag="PLU99_07095"
     CDS             4899..7847
                     /locus_tag="PLU99_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO16859.1"
                     /translation="MSETPTNNAPRDESNRELMARIAELEQRCAAESAERARLQQALR
                     ESEELYRLLVAFSQEFTYWRGPDGRAKYVSPASLELTGYSADEFHSRPELFEELVHPE
                     DRALWAAHSELALRGDPAGELQLRIVTRTGQTRWIAHNCRPIRGAHGEFLGVHASHRD
                     ITQMRQAMQELERARKLFTGGPVVVFRWAAGPGWPCEYVSPNVSSLFGRSAEEFLSGA
                     TPFAEVIHPDDLSRVTAEITQFTQGGAECYEQEYRIVRPDGQVRWLRDFTVVRRDERG
                     HIIHFDGYVLDVTGHKTAEEALQRRARFLSGLSAAAEHLLSSQASVPYQTFVDVLGPA
                     SGASRVYVFRHSRDEAGELHYSRVAEWCAPGVSRQLDNPELQNIPAARLFPEWTRIGM
                     AGGVVNCGLHEASPAARAIMESAGVRRLLLLPLMIDGENAGFIGLDNCTDDRAWEPSD
                     VEFLRAAATDLAQAMKRQRAEAALRDSEEKYRSMMDAMEDLVYICSADLRVEYMNPAM
                     IRRTGRDAVGEPCYRALHDRTEKCPWCPNDLIQQGRSHVWEITSPKDGRTLHASNTPI
                     FRSDGTISKMAIIRDITDIRQAEEQRRKLEARVQQVQKLESLGVLAGGVAHDFNNLLV
                     AVMGYAGLAQSEIAPDSPAQLSLKRIEQAAQRAAELTGQMLAYSGKGHFSVGPVHLNR
                     LVEEMADLLVATVSRKVVLKREFAADLPLVEADATQVRQVIMNLMTNASEALGENDGV
                     ITLRTGIRQVDEAERSSHCLGYDLKPGWYVFLEVSDTGCGMDAETRARIFDPFFTTKF
                     TGRGLGLAAVLGIVRGHRGAIVVDSKPGRGTTFRVLFPVGRNLVAAAVRAQPQVRSHG
                     QNRLVLVVDDEEFVRSLAQEALQRAGFRVLAVEDGPRAIDLFRARYAEIDAVVLDLTM
                     PRLDGAEVFRELYRIDPGVRVILTSGYNEQHATRRFRVSELAGYIQKPYRPHALVEKV
                     CAAIDWPDST"
     gene            7950..8456
                     /locus_tag="PLU99_07100"
     CDS             7950..8456
                     /locus_tag="PLU99_07100"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOO16860.1"
                     /translation="MNQSSRRDFIKKAGTLAAVGAGTTLGAASVQAKQSPTPANMPLT
                     TAGRQIIPGSPYPTFSRAVRLDRLIFVAGVLGQKPGTRDLVSEEFEAQCRQALENLKA
                     SVEAAGSTLDKVLKCNVYLTSAADFATFNKLYVEYFPKDPPARSSVVVKELVVPGAKL
                     EVDCVTYV"
     gene            complement(8585..9904)
                     /locus_tag="PLU99_07105"
     CDS             complement(8585..9904)
                     /locus_tag="PLU99_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO16861.1"
                     /translation="MPANTQGEPDRRQFLRQCLGGAVASAAFGLGVRAPSRDEPPAQR
                     AGPASDDEQFWSNIRRQFPLAADLAYFNAGGLGPSPQPVIDALLRETTALEHDSETGH
                     ERVAAVREKLSAFLNCAPEELALTRNSTEGMNLVARGLSLRAGDEILMTTHEHPGGAM
                     PWLGLAKDTGVRVNLVEPGDGGDDTLARITAALTPRTRVVAVSHILCTTGLCLPARQI
                     AERCRERGLICVLDGAQAVGMVPVDLHALGCDFYVSSGHKWLLGPKGTGLLYIRAGAR
                     ELWRPTNVGAHSDCEYDLDRKVLEFRTQADVIEYGTRNTALLLALGAAVDFLNGIGME
                     RVAQRGRSLALYLRQRLQVIPGIEILTPAAEEASASIITFATTGSGGDVRDWAPLLQK
                     QYRIRPRPVSEHGLRGLRISAHVYNTYAEVDRLVVALSELAKGTRTA"
     gene            complement(10131..10472)
                     /locus_tag="PLU99_07110"
     CDS             complement(10131..10472)
                     /locus_tag="PLU99_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16862.1"
                     /translation="MDNITLTCFSGADFAIGEFAIVDPDVYGACCDEWAGTCADDVGF
                     SDCISVPHWRFSAFATCDELEPPCGEVRGACCYASECVYQRPMECADGGGTYKGDGVP
                     CDPNPCLCVDF"
     gene            complement(11343..11744)
                     /locus_tag="PLU99_07115"
     CDS             complement(11343..11744)
                     /locus_tag="PLU99_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16863.1"
                     /translation="MLKIMFALLEHHEPVSRSPAEGAPEGAGAVHWDLVVEVPGQERL
                     ATWRLSENPIETDAPIAAERIADHRRIYLEYEGRLSGGRGWVRRVDGGDAQLCISGDI
                     EGQVLLELRGHLLKGKFRLISAGLGLSFRRA"
     gene            11934..>12802
                     /locus_tag="PLU99_07120"
     CDS             11934..>12802
                     /locus_tag="PLU99_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOO16864.1"
                     /translation="MADTTSDYLLQRIYDWGVRLIYGFPGDGINGILGALDRAKDRIR
                     FIQSRHEELAAFSACAHAKFTGQVGVCMATSGPGAIHLLNGLYDAKKDHQPVVAIVGQ
                     SATFALGADFQQEVDLQTLFKDVADRYVGTVQSPVAVRHVVDQAFRIAIAERTVTCII
                     FPADIQEQSAVESPPRKHGATFSGVGYLRPEVVPREQDLRAAARVLNEGEKVAMLVGA
                     GALNATDEVLEAADILGAGIAKALLGKAAVPDDLPFVTGSIGLLGTRPSWNMMMNCDT
                     LFMIGSGFPYSEFL"
BASE COUNT         2439 a         4162 c         3925 g         2276 t
ORIGIN      
        1 ggtttgcggc gcagtctttc cagaaaagtt tcgaccccca gcagcagcaa cagtacggca
       61 ttggtgacga accccatcca gccctcaaac agcggcttga tgaagctgta ggtcggcacg
      121 cagccgcagt cgcccccgtg cccggccgcc ttggcgacaa tgaacaccac caacatcagc
      181 gccaacagaa tacgggcctc gcgacgccag acggacgcta tcaacagcag gccggcaatg
      241 atctccaccc acggcagaac cagtgcggtg aagttgctca aagccaatgg catgagctca
      301 taactgcgga cctctttgat gaacgtcgca gggtccatga ccttggtgta gccggtgtat
      361 atgaaaagca acgccacggc gagccgggcc gtccattcca gaactgccca ccctatccag
      421 ctcgcattcc ggccagacgc cgcggtggtc atggggcctc ctgttcttct tccgcgcccg
      481 gcgaatcgtc gccctgcggc tcatcgccgc tctcggcagc ggcgtccgca taatcctcgg
      541 cgacggacgt ctctacttcc tcactctgcc gccacggatc gtcctgaaac atgtcgggcc
      601 cgcaggcgat cggcaacttc gcctcccgaa tgcctaccca gccggggtga aagacgcgca
      661 gcgtctcggg cgggaaggcc caggagcgca tgtcattcca cagttcctgg gccgattcac
      721 agtccgccga agaacaatag atcacgatcg gcgtcccgat gaaagacaag gcccgatgaa
      781 ggcagtcgtg cgcggcctcg gccggtatat tcatgattaa cggggcgtcc aggtgcccct
      841 cctcaaacgc ctccggcgcc cgcgcgtcaa tgacgagcct gcccgcctgg tagtgccgca
      901 caaaatccgc cagatcaatg ccggtctgct gcacccaaat agcgcgttgg tcgcgttctt
      961 ctttgatctg ctgcacgtcc ggcaccagcg gtatctgccg gtagcggccg taaccgacgc
     1021 caataagcgt cgcgcccgcc agaagcacca ggattctcac gcccgatcgc agcacggctg
     1081 ttgctcctgt catacagaag atagcggctc agccatcatc attatcgttc tgctccatca
     1141 ttctcgctcg actccgctgc cggcgaagcg gccggcgtgc ccggctgcac atcccggacc
     1201 acgacgcgca gctcctggaa gcgcggttca gccgaatcgg tgagcacggt gatgaactgg
     1261 tcgccgggcg gcaactggtc gcccgggggc aaaatcacac ggatgggctg ggcggtccag
     1321 tcgctgcctt cgcgtgccgg ccgcggcgac aacagctcaa aactgatgct gggcgtgctg
     1381 ggccggactt cagtgatccg gatcggcttg tcggccctgt aagtcacggt gatttcttct
     1441 tctttggggg ttataagcat gcgctgggcg cggagcacct ttgggcggat cgaaacgtcc
     1501 tggagcaccc gggcgtgaac cggaatgtga atctccggcg agcgttccac gtccgtttca
     1561 atcaccaccg tggtgcgcgc gaagtcgtcc gttaagggcg gcttcgtcgt ggcgacgagc
     1621 tcataggcca cgccgggctc gacctccttc agctcgatcg tgaacggccc gaaggcctga
     1681 tccggacgca acttcagatg gagcggcccc tcgtatttgc tttcaatccg cagcgtctgg
     1741 gtgacctgcg agtcactcgt caaacgatcg aagaccagcc cctccgccgg cgacgtgctg
     1801 tacaacggct tcacctcgcc ggtcaccgca atgcgcacat tcggctgatc cgggtcgttc
     1861 gttgtgagag tgacggtttg gttcgctttg cccttgcgtt tcaaggtgtc gtaagtgatt
     1921 cgaaacgtgt ctgattcgcc cggctccagt ggcgaacgcg gcttactggc caccgtgcaa
     1981 ccacacgaac ttttgagcgt cagcgtgagc ggcgcggtcc cgacgttcct gacggtgaaa
     2041 tcgccggcag cccgctcgcc ctgccagacc tcgccaaaat tcaaggccgt cggcgagagt
     2101 tccaggcgcg gcacgccgct gctgtcagcc gattcgggca cccctgcggc gccctctgcg
     2161 gcggggccgg ccggctgggc aagggcgttc tcacaagtga gtgcagacag caccgcgccc
     2221 agcgcaagca tcagcacggc gcttctggga ttgacaagga cggcaatcaa cgtgttcatg
     2281 aacagctccg gtgaagcagt ctccttttcg gccaaagcgg tcagggcgtc tttcgttggc
     2341 tccggccgcg ggcacgcgcg cttagatcgc gatactcaac agtgtcggtg gtggcataga
     2401 ttgtcagcgt gggatactcc gggtctgtcg tgtgcacccg aatcggcagg ccgccgggcg
     2461 ggaacaccgt ctgcggaggg aactccgcca caaacttcac gattgaggtc ggcctgcgtc
     2521 ccgcgcggtt tgcctccggc gcaaagggcg cgcccaatcg tacgatagcg tgcgggtcat
     2581 cgcactccgc cttcgtgatg cggaatgcgg gatcattccc gaagtactcg atctccccag
     2641 tggcctcgag agtttcgcca cgctcgccga tcaccagcag ggccggctga agaaaattca
     2701 cgcggtcgat ttgcaggact tgcgtggata tctccaggcg cggctcgtct tccagaccag
     2761 tggtcacatg cagcatatct gccatccgcc gctgcgtgat cggcggcctg gtagtgatca
     2821 cgatttcgta ctcctgaccg gcgcgaatct cgttcagttc ggccgtgaac ttcccagacg
     2881 cgtagtcctg cagcttcaac tgcatgggtt ggggggtggt gttcacgacg cgcacccgcg
     2941 tggacattag ctcgtccggg ctaagaagac gaaagacggc gaattcaggt tctatcttga
     3001 cggcgcgctt aaccttgcct tccacgcgca aggccttctt aggctcggac gtgtcgttgg
     3061 actcaatcca cacgttggcc gaggtcgggc cgtttttgcc gcgcgtgttg aaagtgatct
     3121 ttagcggcgt ggattcgccg ggcaggagta cacgcttttc cggctgagcc gcggtgcacc
     3181 cgcagcttgt gcttacgttg gaaatccgca gctccgcggt accggtattg gagagctgga
     3241 cggtggtctc aaggtgctcc aggtgccgcg ccgtgccgaa gttccaggag tcctgggaca
     3301 gcttcagccg gggtccgttt gccggcgcgt ctggggtttc agcgggtttg gcaggcggct
     3361 cggacgcggt tgcgaccagc aacgcgatta ccggccgcaa aaaacagaga aacggcatca
     3421 aaaacctcca tggcggacgg cgggcatctg aaagatccgc aagatgcgca acgacacccg
     3481 gccggcgcct ggtcgccctg gcgctgcggc ggacgacccg attaaagacc tccaagttta
     3541 gcaaaggtta gtcgactgac cagagtcgga cgaaccggtc gaaagaggca acgagccaga
     3601 gaactctata tgagctgact gatggggccg atgagcatcg tagctgcgcg ggctccaaac
     3661 gccctccagg agcgataagc gatagctgtc cgataaattc tcacggcgcc ttaatccggt
     3721 cggcgtggcg ggccgtcgga tcatcgcggc accacccgca tcagcaattc gagcgttctt
     3781 tgcaggcttg taaaagcggc gaatcccggg tactttggtt gccgggcgcg cccctttttc
     3841 gccgatcacc gcgccgcggt gaccaggagt tctgatgaac ggacgcttaa accgcctgtg
     3901 ggccggtttt ctgttgctcg tgctggccgt acccgcctgg gccatcaaac tcggtgaccc
     3961 tgctccgccc atacagatcg ctcagtggat caaaggggag ccagttgacc ttgccgccgg
     4021 caagggcaag aacgtctatg tcctcgaatt ctgggccacg tggtgccagc cgtgccggga
     4081 aagcatccct catctgacgg agcttcaaaa gaagtacaag gatcaagggc tgatcgtcat
     4141 cggaatcacc gacgagaaca accccagcgc cgtgcccgcg ttcgtcgcca agatgggcga
     4201 caaaatgaac tacacggtgg cctacgacga caatcacaag acgaacgccg cttatatcca
     4261 ggccttcggt ttgcggggca taccgcacgc gttcgtgatc gaccgtgaag ggcgtatcgc
     4321 ttggagcggg cacccgaaat tcgggctgga acaggctgtg gaacagatca tcaccggcaa
     4381 atacagccta gccgccgcca ggacccgcga ggaaacctta cccttgctga cgcgttatta
     4441 cgaactcgcg tcaaccggag gggacgaaac cgaactgaaa agcctcgggc gccagatcgt
     4501 ggacaaggtg tcgggcgatg cgcagattct ggcgatgttc tcgttcgata ttctgcacaa
     4561 gccagagttt aagtatcgcg acctggagct ggcgctagac gcctccaagc gggcctgcga
     4621 actggcgggc gaacgcgctg acgtggtcgc cgtttacgcc cgtgcgcttt gggacaacgg
     4681 caagaaagct gaagctgttg aatctcagcg gagagccgtt gccttggccc aagataaccc
     4741 gcggattcgc gcggagctgg agaaagctct agccgcttac gaacagcaac tcaaggccca
     4801 gccatagctg gtctgccggc cgccacgctg cacgcggatc cctggttgac cgctgcacct
     4861 ggaagaagat agcattagcc tagttgtggc tggccaacat gtccgaaact ccgacgaaca
     4921 acgctccgcg cgacgagtct aatcgtgaat tgatggcacg catcgcggaa ctggagcaac
     4981 gctgcgcggc tgaatccgcg gaacgggcgc gccttcagca ggcgttgcgc gaaagtgagg
     5041 agctgtatcg cctgctcgtg gcgttctcgc aggagttcac gtattggcgc ggtccggacg
     5101 ggcgcgccaa gtacgtctcg cccgccagcc tcgaactcac gggctacagc gccgatgagt
     5161 ttcacagccg gcctgagctt tttgaagaac tcgttcatcc ggaagaccgc gccctgtggg
     5221 ccgctcacag cgagttggca ttgcgcggcg atccagccgg cgaactccaa ctgcggattg
     5281 tcacgcgcac cggacagacg cgctggatag cccacaattg ccggccgatc cgcggcgcgc
     5341 acggcgaatt cctcggcgtt cacgccagcc atcgcgacat cacccagatg cggcaggcca
     5401 tgcaggagct ggaacgcgcg cggaagctgt tcaccggcgg tccggtggtt gtcttccgct
     5461 gggcggccgg cccgggctgg ccgtgcgaat acgtttcgcc caacgtgtcg agcttgttcg
     5521 ggcgcagcgc cgaggagttt ttgagcgggg ccacgccgtt cgccgaagtg atccacccgg
     5581 acgatctatc acgcgtcacg gccgaaatca cacaattcac gcagggaggg gccgaatgct
     5641 acgaacagga atatcgcatc gtgcgcccgg acggccaggt gcgctggctc cgggacttca
     5701 ccgtcgtgcg ccgtgacgag cgcggccaca tcatccattt cgacggctat gtgctggacg
     5761 ttaccgggca caaaacggcc gaagaggcgc tccaacggcg ggcacgcttt ttgagcggac
     5821 tgagtgccgc ggccgagcat ctgctgtcat cgcaggcgtc ggtcccctac cagacgttcg
     5881 tggacgtttt gggaccggct tcgggcgcca gccgggtgta tgtcttccgg cactcgcgcg
     5941 atgaagccgg cgagctgcac tacagccggg tggccgaatg gtgcgccccg ggcgtgagcc
     6001 ggcaactcga caatccggag ctgcagaaca tcccggcggc gcggctgttt ccggagtgga
     6061 ccaggatcgg catggcgggc ggcgtggtga attgtggctt gcacgaagcg tctccggccg
     6121 cccgggccat catggaatcg gcgggggttc gccggctcct gctactgccg ctgatgatcg
     6181 atggcgaaaa cgcgggcttc atcggcctgg acaactgcac cgacgaccgc gcctgggagc
     6241 ccagcgatgt ggagttcctg cgcgcggccg ccaccgacct cgctcaggcc atgaagcgtc
     6301 agcgggccga ggcggcgctc cgcgacagcg aggaaaagta ccgctcgatg atggacgcca
     6361 tggaggactt ggtctacatc tgctccgctg atctgcgggt ggagtacatg aacccggcca
     6421 tgatccgccg gacgggccgc gacgccgtcg gtgaaccgtg ctaccgcgcc ttgcacgatc
     6481 gcacagagaa atgcccgtgg tgcccgaacg acctgattca gcagggccgc agccacgtct
     6541 gggagatcac cagccccaaa gacggacgga cgctgcacgc cagcaacacg cccatcttcc
     6601 gctctgacgg cacgatttcg aagatggcca tcatccgcga cataacagac ataaggcagg
     6661 ccgaggagca gcgccgcaag ctcgaagcgc gcgtgcagca ggtgcaaaag ctggagagcc
     6721 tgggcgtctt ggcgggcggg gtggcacacg atttcaacaa tctgctggtc gcggtcatgg
     6781 gctatgccgg tctggcgcaa tcggagatcg caccggattc gcccgcgcaa ctgagcctga
     6841 agcggatcga gcaagccgcc cagcgcgcgg ccgagcttac gggacagatg ttggcctact
     6901 ccggaaaggg tcatttcagc gtcgggccgg tgcacctcaa ccgcctggtg gaagaaatgg
     6961 cagatttgct ggtggccacc gtttcgcgaa aagtggtctt gaagcgtgag ttcgccgccg
     7021 atctgccgct ggtcgaggcg gatgccacac aggtgcggca ggtcatcatg aacctgatga
     7081 cgaatgcctc ggaagcgctg ggcgaaaacg acggcgtgat tacgctgcgc accggcattc
     7141 ggcaagtgga cgaagccgag cgctcaagtc actgcctggg ttacgatctg aagccgggtt
     7201 ggtacgtgtt cctggaagtg agcgatacgg gatgcggcat ggacgccgaa acccgggcgc
     7261 ggattttcga tccgttcttc actaccaagt tcaccggccg cgggctgggt ttggccgccg
     7321 ttctgggaat agtccgcggg catcgcggcg cgatcgttgt cgacagcaaa ccgggccgcg
     7381 gaacgacttt tcgcgtgctg tttccggtgg ggcgcaacct cgtcgctgcc gctgtgcggg
     7441 ctcagccgca agtgcgttcg cacggccaaa accgcctcgt tctggtagtc gatgacgagg
     7501 aattcgtgcg ctcgctggca caagaggcct tacaacgcgc cggattcaga gtactggctg
     7561 tcgaagacgg gccccgcgcc atcgatcttt tccgcgctcg atacgccgaa atcgacgccg
     7621 tggttctgga cttgaccatg ccgcgcctcg atggcgcgga agtattccgc gaactctacc
     7681 gcatagaccc tggcgtgcgc gtcatcctga ccagcggcta caacgaacag cacgccacac
     7741 gacgctttcg cgtctccgaa ctggcgggct atatccaaaa gccatatcgc ccacacgcgc
     7801 tggtcgaaaa agtatgtgcg gccatcgact ggccggattc cacttgatcc ctcgatccct
     7861 tgatccctag atccctcgat ctccccaacc gcgccattcc gcattccgca ttccgcactt
     7921 atcattcccc gcagagaagg agtttccaaa tgaaccaatc ctcgcgacgt gacttcatca
     7981 agaaagccgg aacgctggcc gcggtcggcg ccgggacgac actgggggct gcatccgtcc
     8041 aggcgaagca atcgccgacg ccggctaaca tgccgcttac gacggccggc cgtcaaatca
     8101 tccccggcag tccctatccc acgttctccc gcgccgtgcg gctggaccgc ctgatctttg
     8161 tggccggcgt gctcggccag aagcccggga cgcgcgacct ggtgtcggag gagtttgagg
     8221 cgcagtgccg gcaggcgctg gagaacctga aggcgtcggt cgaagccgca ggttcaactc
     8281 tcgacaaagt gctcaagtgt aatgtatatc tcaccagtgc cgcggacttc gccacgttca
     8341 ataagctata cgtcgagtac tttccgaaag acccgccggc ccgctccagc gtagtagtaa
     8401 aagagctggt cgttccgggc gccaagctgg aagtcgactg cgtcacctac gtctgacctc
     8461 gaccggcgtt cggcgcgggc gttcaggaag ccacagcttc gagcaaggcg gtccggtgtg
     8521 ccactgcttg acagcgcagc tttaagcagt gtgagtgtgt gtgctaacgc ttgaaagcgc
     8581 agcttcaagc agtgcgggtc cccttcgcca actccgacag cgccaccacc agccgatcga
     8641 cctcggcata ggtgttgtag acgtgtgcgg agatccgcag cccgcgcaag ccatgctccg
     8701 acaccggtcg cggacgaatt cgatattgtt tctgcagcaa cggcgcccag tcccgcacgt
     8761 cgcccccgct cccagtcgtg gcgaaagtaa tgattgaggc tgacgcctcc tccgccgccg
     8821 gagtcaaaat ctcgatcccc ggtatcactt gtagccgctg cctgaggtac aacgccagac
     8881 tgcggccgcg ctgcgccacg cgctccatcc cgatcccgtt caggaagtca accgccgcgc
     8941 ccaacgccag cagcagcgcg gtgttgcgcg tgccgtactc aataacgtcg gcttgcgtgc
     9001 gaaattccag cactttgcga tccagatcgt actcgcaatc ggagtgcgcc ccgacattcg
     9061 tcggccgcca tagctcgcgc gccccggcgc gaatatagag caaccccgtg cccttcggcc
     9121 ccagcagcca cttgtggccc gacgacacgt aaaaatcgca gcccagcgcg tgcaaatcaa
     9181 ccggaaccat cccgaccgcc tgggcgccat ccagcacgca aatcagcccg cgttcgcggc
     9241 atcgctcggc aatctgccgc gctggcaagc ataggccggt agtacacaga atatggctga
     9301 ccgcgacgac gcgtgtgcgc ggcgtcaagg ccgcggttat gcgcgccagc gtgtcgtcgc
     9361 cgccatcgcc cggctcgacc agattcacgc gcacgccagt gtccttggcc agccccagcc
     9421 agggcatggc cccgcccgga tgttcgtgcg tcgtcatcaa aatttcgtcg ccggcccgca
     9481 acgataggcc gcgcgcaacc aggttcatgc cttcggtcga attgcgcgtg agcgccagct
     9541 cctccggcgc gcagttgagg aaggcgctca acttctcccg aacggctgcg acgcgctcat
     9601 ggccggtctc ggagtcgtgc tcaagcgccg tcgtctcgcg cagcagggcg tcgatcaccg
     9661 gctgcggcga tggcccgagc ccgcccgcat taaagtaggc caggtcggcg gccagcggaa
     9721 actgccggcg aatgttgctc cagaattgct cgtcgtcaga agctgggccg gcgcgttgcg
     9781 cgggtggttc atcgcgcgac ggggcacgaa cgcccagccc aaacgccgcc gaagccaccg
     9841 cgcccccgag gcactgccgc aggaactggc ggcggtcagg ctcaccctgc gtatttgccg
     9901 gcatgacgca cccaaaccct acgctagctg aaagaggtcg cgcggacgta gtgccgactt
     9961 ttacgagaag tcggcgcctg cgccaaggcc gagtttgctt cacgatcccg cgcggccgga
    10021 accaccctgc cacaggtgct gccggtcagt acggtcggcg caccgcctcc gggagtacgt
    10081 aaagacccgc tcagggcgcc ggcggagcgc ggatcgccct ccggaaatac tcagaagtcg
    10141 acgcacaggc aggggttcgg atcgcacgga acgccgtcgc ctttatacgt gccgccgccg
    10201 tccgcgcact ccatcggccg ctggtaaacg cattcggaag cgtagcagca ggcgccgcgc
    10261 acttcgccgc acggcggctc cagctcgtcg caggtggcaa acgcgctgaa ccgccagtgc
    10321 gggacactta tgcagtccga aaaccctacg tcatcagcac aggttccagc ccactcatcg
    10381 cagcatgcgc cgtacacgtc cgggtcaacg atagcgaact ccccgatcgc gaaatcagcg
    10441 ccgctgaaac acgttagcgt gatgttatcc acgtagcttc cgcacacacc gacataggta
    10501 gcgccgcccg agccatgcgc cgtaaatggt tcagactcgt accgattgtt gaccacgacc
    10561 ccagggcaac gcagtcgtat agcagcatgt ggcggcactc gtgcgcgtgc gagccgctct
    10621 atcgcgcttg ctgctatgag gccgtttcgc cggctttctg cctcgacgct gtcgacatca
    10681 tggattgcct cgccacgggc gggcgaccgt aggcgaagga tcaggcccga acggggtcat
    10741 gagatacgtg cccagcgtat ccggcggggc cgccgacccg acgctggtgt cgaacccgta
    10801 caaccacccc gccgcagccg ccggctgaac aatgaagctc gtcgcgcctg gcgccgcact
    10861 cgcctgagcg gtgcaagtca gtgcaattgt cggggccgct cccgcattcg aagccgggtc
    10921 gcttctgtgc gccgtgccca ggttcgcccg aggctggctt tggcggccgc tgttggcagg
    10981 caggttttgg gcggcggcgt tcccggtagc caaatacctc ctgaattgcg tagtgctctc
    11041 cgagactcga tcgcgctcag tgccatgctg agcgttcgtg caaacccagt gtaacacaat
    11101 ctgtggcgcg ctaggcacta ctttttcaac tggcgcagcc cgcctcgcag aggcgcacat
    11161 cttggctggg cagacactta cgtgctgagc cgtccggtcc gcgccctctc acaggccgca
    11221 caaccagcaa tatgcacacc ccgtgattgc cgcagaaggc cagctccgcg ccagtatggc
    11281 agttccattc cagaggccgc aacacgcggc ccggacctgc ggacgctatc tgacgcggac
    11341 tttcaggcgc ggcggaagct gagtcccaag ccggcggata tcagcctgaa tttgcccttc
    11401 agaagatgac cgcgcagttc aaggagaact tgtccttcta tgtcgccgct aatgcacaac
    11461 tgcgcatcgc ccccatcaac acgccgcacc caaccgcggc cgcccgacag ccgaccttca
    11521 tattcaaggt aaattcggcg gtggtccgct atacgctcgg ccgcaatcgg ggcatccgtc
    11581 tcaatcgggt tttctgaaag gcgccacgtg gccaggcgct cttgacccgg cacttcaacc
    11641 accaggtccc aatgcacggc gcccgccccc tccggtgctc cttccgcagg cgaacggctg
    11701 accggctcat gatgttccag caacgcaaac atgatcttca gcataacacg ccaactcgca
    11761 tcacgcccag cgcctgcggg gcgacgctcc ctgagaacct gacacgcagc aatcacaact
    11821 ctgagcccca tttcatccga aattcacggg tgattcagcg gcccgccccc ataatcgtgc
    11881 ggtctctgcg acccgtggtc cgcccagcgg accctacaaa cgagggcttt accatggccg
    11941 acacaaccag cgactacctg ttgcagcgaa tttacgattg gggcgtgcgc ctgatctacg
    12001 gctttcccgg cgacggcata aacggaatcc tcggcgcgct cgaccgcgcc aaggacagaa
    12061 tccgcttcat ccagtcgcgt cacgaggaac tggccgcctt ctccgcctgc gctcacgcca
    12121 aatttaccgg ccaggtgggt gtttgcatgg caacgtcggg gccgggcgcc attcacctgc
    12181 tgaacggcct ctacgacgcc aagaaagacc atcagcccgt ggtggccatc gtcggccaat
    12241 cggccacgtt cgccttgggg gccgactttc agcaagaggt tgacctgcaa acgcttttca
    12301 aggacgtagc cgaccgttac gtgggaacgg tgcaatcacc cgttgccgtg cgccacgtgg
    12361 tcgatcaggc gttccgcatc gcgatcgccg agcgcaccgt cacgtgcatc atctttcccg
    12421 cggacatcca ggagcaatcc gcggtcgaat ctccgccgcg caaacacggc gcaaccttca
    12481 gcggagtcgg ctatcttcgc ccggaagtag tgccccgcga gcaagacttg cgggccgcgg
    12541 cgcgcgtact gaacgagggt gaaaaagtcg ccatgctcgt cggcgcggga gcgctgaacg
    12601 ccactgacga ggtgctcgaa gcggcggaca ttctgggcgc gggcatcgcc aaagcgctgc
    12661 tgggcaaggc cgccgtgccg gacgatctgc ctttcgtgac cggctccatc gggctgctgg
    12721 gaacgcgccc gagctggaac atgatgatga actgcgacac gctgttcatg atcggctcgg
    12781 gatttccgta ctccgaattc ct
//
LOCUS       DAOSYS010000087        12661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4177_length_12661_cov_5.375853, whole genome shotgun sequence.
ACCESSION   DAOSYS010000087 DAOSYS010000000
VERSION     DAOSYS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_12661_cov_5.375853"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLU99_07125"
     CDS             complement(<1..252)
                     /locus_tag="PLU99_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16865.1"
                     /translation="MSTQAQRVRAEQRPPAGTSRAEKQLSWWTRGKFGLVRWFLFAWA
                     RLFSLKGLYLFGCWFATAEYLINFKRRRRYWRELQGVFPE"
     gene            complement(476..1705)
                     /gene="pepT"
                     /locus_tag="PLU99_07130"
     CDS             complement(476..1705)
                     /gene="pepT"
                     /locus_tag="PLU99_07130"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002648531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOO16866.1"
                     /translation="MDTLLDRFLRYVKVETTAVEDTDKYPSSPGQLELGRMLAEEMRA
                     IGLSDVSISEAGVVMGTVPGNVRGAPTIAWLAHQDTSPESSAKDVKPNIIKNYDGQDI
                     VLPGDKTKVIKVAETDGLAELKGKTLITTDGTTLLGADDKAGIAVIMTAAETLMKDPQ
                     HKHGPVRVVFTVDEEVGRGTDKVDIAKIGATAAYTLDGEAAAGIENETFSADMATVTI
                     TGVNIHPGFAKGRMVNALRLAGQFLTRLPWHRLAPETTEKREPFLHPYVIEGGVPEAK
                     IRILLRSFETAELAELAKVLQTIAATLEAEHPRAKINVEVKKQYRNMREHLEKEPRAV
                     KLAVEAYRRIGVEPLYQSIRGGTDGSRLSEMGLPTPNLSCGMHNFHSPLEFACLEEME
                     TAVKALVELARLWGEQK"
     gene            3154..5484
                     /locus_tag="PLU99_07135"
     CDS             3154..5484
                     /locus_tag="PLU99_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16867.1"
                     /translation="MATGAAALLEGFGDVAPGNVLRERYRLVDVIGRGAHGVTYFAEH
                     EFLNHPCVLKILPQRIRDASDSAVQRLRYEARAGFRVNNPHVVRVLDCDVINDVWYFV
                     MEFVDGIDLATLIENGVSLCWQQAAGLAIDSATGLMAIHNAGLLHRDIKPANLLLGAD
                     GRLRISDLGVAGMASRRAGGSSSPGAVGTLAYAAPEMLIPGATVDARADLFSLGATLY
                     HAVTGKRPFANESVFATLINTQTRAVEWPEQAPANVPAWFIKTILTLLANDPAQRFAS
                     AEALRNHLARPQQQPATVAAPAVSSNGVQPRGLAVLPFENESGEGADDWLGFALADTL
                     ARELSRIPGTYVADHEQLRAVLQRPEIAAVGGLNEQLLAAGRVVGAGTIVTGRFRRGQ
                     ESICFWSDVVRRSMPQPERIGPLQGPLSRMVELQSLLFTRLAQLLQLNPGAAGVGAAL
                     PKVALETREKFTRGKQAYLQGDYETAIRLARDVIEEDADFIEALQYLSACSARLGRYE
                     EAGKYHRQITSLAEQRGDKQLLVEAKANMGVMYYLKGEYEQAQRLYTEAAVIAEGLGL
                     EAERAQIYNNAGFALARLGRSEEAEAAFRRAVDVCKGFGALAPLIGPYNGLGNLLLDQ
                     QRYAEARDYYQRALTLAQEIGDRTNTGISHMHLGRCASLEGQFASAKNEFALALNTLE
                     GTSFWNARTITYEYMAEMNLRLGDYAEAVRCAEKKIELARRHANRTMEAAAWRQKAEA
                     LRLSGRAREADECLLQARAAQPTGSQPADSGATEPA"
     gene            5511..7925
                     /locus_tag="PLU99_07140"
     CDS             5511..7925
                     /locus_tag="PLU99_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO16868.1"
                     /translation="MFPIVLLILLNVAVLGVIVLVLLELRRLRAVRKSGVESRQIQQL
                     KDLLSAIPDPVIVLDSKGRRVKNHSGNDHGEAAALLDKLPEPERNALNEKVALAIKQR
                     QPGSQRMLLDGRSIMAQIVPLPNAQDEAAVWLRDCTDQEVSATRAGKLAMRNRAILRS
                     AMDGFFVVDENYRFVEVNDSFCRMTGYSPDELLQMKITDLEVKEPANRRADVSPMRTG
                     LHQFATAHRHRDGHIVRLETSVIVLRDDDHKILVGFARDVTERLRDQERLEQLSRQNK
                     QILDCAGEGIYGVDLDGRISFANPAAARMLGWEVHELIGMCAHDLMRRGQAQPAPMLA
                     EGCPICEAVRGGIVRQGVDTLFRRKDGTELTVDFISSPIRQHGQLTGAVVVFRDTSER
                     KRAENERRQLEARIQQSQKLESLGMLAGGLAHDFNNLLLAVLCNASLAIERLPPDSEA
                     CAFLNKIVKSGRRASELTRQMLAYAGRARYDVQPLELNSLVEEMVDLVGAALPKQVKV
                     ELNLAPDLPLVEGDASQLQQILMNLFMNGAESLGSQPGTVSARTSLRTLSAAELAADY
                     IGNSLAPGDYVCLEVADTGCGMSPAVLARIFDPFFTTKATGRGLGLSALLGIVRSHSG
                     AVRVISAPGQGTTFTLLFPPAAADAGLRPPRQPVTELPPGATILVIDDEEDIREVVQA
                     VLESRGARVLLAKDGAAGIEQFRRCAADISAVLLDMTMPGIGGEAVLEEILAIRPDAR
                     VLLSSGFGEEEALARLRDSGAAGFVPKPYTAQALVDRIGAALGARATPLESAATPVAA
                     DKPDSG"
     gene            complement(7917..8915)
                     /locus_tag="PLU99_07145"
     CDS             complement(7917..8915)
                     /locus_tag="PLU99_07145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO16869.1"
                     /translation="MLFRLPRVVRGRFLTACLPAVGLFALLWGAPACAQDGDAMAIVN
                     GQPISRKQVVDKLIETHGLAVLQQMIALDLAKQESQRRGLTVTPADIQAQFDRALEEI
                     VPTSDSRGVTLTPESKQQALQTILQQRCLTLPEFMIAMERNAHLRKLVEADFTVDEAT
                     LREEFARLYGEKVQVRHIQVAAGDLRALNEALDQLSRGVDFAEVARAVSINQDTAARG
                     GMMEPFTFTDTEIPAALRDYAFSLSVGQVSAPLQTGQYVHILKLEGRVPPTDVTLDDV
                     RDEVERQLRARVERKKMNEMITRLYQQAKIRVLDPQLKGDFEKLLDDFKSGRATSP"
     gene            9179..11320
                     /locus_tag="PLU99_07150"
     CDS             9179..11320
                     /locus_tag="PLU99_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOO16870.1"
                     /translation="MFRRRLTWFWILLTALACALIARLVDIQALHAEEYRDLAARMLT
                     QPVRYLRAPRGSILDRNGRVLVSHEPSFDISAHFAVLTGRSSEYLHALARELRRRGEY
                     PESMSLDEIAGRLRLQIAATWQRLSELTGRPVSEFIERGEEIRQQVARIRMAVRRRTG
                     IDQPVAEESDPLLAMHPLIEDVDHETALKVRLELEAYPWLSVAPGSRRVAHDADAAVH
                     LLGRLGAVSRERQQEDPLAGDELRRLRAGESCGITGVERLAETTLRGTRGRIVEELDG
                     TLVERSEPVAGQDVELTIDMELQEFVLAALREAVEGTETTPGVPYASGASAVVLDVAT
                     REVLALVSYPAYSYERFDEDYDGLVRDARRTPLRFRAVSGLYPPGSTCKAITLVGALS
                     EGETTPEERIHCTGHLLPELENQFRCWIYNDYRTTHDARQPEGQRGEDAIRNSCNIYF
                     YKLGGRLGPERLCNWFSTFGLGRLQGTGLIEEAPGVVPTERWLRANQRRGFERSDPWN
                     WSIGQGEVAATPLQIANVGATIASGHWAPVRLLRDAPPAPADQIVKLPPDALRVLRTG
                     MWRVVNENGTGKAAKLKSDRYVLCGKTGSAQAVPTPVRYRYIFEWPDGRRETREAYLE
                     EDAAASFGAERPRCIGRRTSARFPELEEGESIAHAWYMAYTQPASTPRGAAPQGPVYA
                     ISTVIEFGGSGGRVAGPVVKKIAEYLLERDH"
     gene            11321..12568
                     /locus_tag="PLU99_07155"
     CDS             11321..12568
                     /locus_tag="PLU99_07155"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOO16871.1"
                     /translation="MTAPASPLNLTLLGWGIALPVAFLAAVGLACIHATEANDPVAPV
                     GLVATPAVEPQAAVLERVIQAIGPLTLRQMFYLVTGVLLMLAALVPSYQKIGRWAYPA
                     YAILLVLLVLLVVDIYVDLPLIPVRNNARRWLAWGPLSVQPAEFMKVGLTLTLARYLR
                     FRSSYRSLRGLLPPLFLTLVPMVLILREPDLGMMLMLLPVLFLTLFVAGARLRHLALI
                     ILLGCATLPVFFIYGMKPYQRDRVLTVIKQNTADERWHRGPGYQLRQSKIALGSGGLT
                     GEGWGRGVFLESRVRLLPEEHNDFIFAIIGHQFGLVGCVLVILAYGVIVFFGIEVATA
                     TNDPFGRLLAIGVVVMIVAQALLNIGMTVGLAPITGMTLPFVSAGGSSLWANFLALGL
                     LVNVAQRRPMLIANPPFEHAEEE"
BASE COUNT         2229 a         3933 c         4208 g         2291 t
ORIGIN      
        1 ttccggaaag accccctgca actcgcgcca gtagcgccgc cggcgcttga agttgatcag
       61 atactccgcc gtcgcaaacc agcaaccgaa cagatagagc cctttgaggc tgaacaggcg
      121 cgcccacgcg aacaggaacc agcgcaccag cccgaacttg ccgcgcgtcc accaactcag
      181 ttgtttttca gcgcgcgacg ttccggcggg cggccgctgt tccgctcgca cacgctgggc
      241 ttgcgtgctc actgcggttc cttcgactga catccgcccg gccgtctcct tctttccgcc
      301 cgcataccgc caagcattct ggggttttca gaagagccac gcaacgggcg gcaggcgtac
      361 tcgccggtct ccagcggcgg ctcaacatcc cattttcgga cagggctatc tacgtgtcgc
      421 ccagaccgag ctttgggggc tccgtgaatg cttatggtcc gttgggctat cgcgactact
      481 tctgttcgcc ccacagacgg gccaactcga ccaatgcctt taccgcggtt tccatctcct
      541 ccaggcacgc gaactccagc ggcgagtgga aattgtgcat cccgcacgaa aggttcggcg
      601 tcggcaaacc catctcgctg agccgcgagc catccgtgcc gccacgaatc gactggtaca
      661 gcggctcgac gccgatgcga cggtaggcct cgactgccag cttgaccgcc cggggctctt
      721 tctccaggtg ctcgcgcatg ttgcggtact gcttcttcac ctcaacgttg atcttggcgc
      781 gcgggtgctc ggcctccagc gtggcggcga tcgtctgaag caccttggcg agctcagcca
      841 gttcggccgt ttcgaaactg cgcagcagaa tgcgaatctt cgcctcaggc acgccgcctt
      901 cgataacgta cgggtgcagg aacggctcgc gcttttcggt cgtctcagga gccaggcgat
      961 gccacggcag gcgcgtgagg aattggcctg ccagccgaag ggcgttgacc atgcggccct
     1021 tggcgaaccc gggatgaata ttcacgcccg tgatcgtgac ggtggccatg tcagccgaaa
     1081 aggtttcgtt ttcaatgccg gctgcggcct cgccgtcgag tgtataggcg gcagtggctc
     1141 cgattttggc gatgtctact ttgtcggtgc cgcggccgac ctcctcgtcg acggtaaaga
     1201 cgacgcgcac cgggccatgt ttgtgctggg gatccttcat cagtgtttcg gcggcggtca
     1261 tgatgacggc gatgccggcc ttgtcatcgg cgcccagcag cgtggtgccg tcggtggtga
     1321 tgagcgtctt gcctttgagt tcggccaagc cgtcggtctc ggccaccttg atgaccttcg
     1381 tcttgtcgcc gggcagaacg atgtcctgac cgtcgtagtt cttgataatg ttgggcttaa
     1441 cgtccttggc ggaggactcc ggggacgtgt cctggtgagc gagccatgcg attgtaggag
     1501 cgccgcggac attgcccggc acagtcccca ttactacgcc ggcctcactg atactcacgt
     1561 cactcaggcc gattgcccgc atctcctcgg cgagcatccg gccgagctcg agctgtccgg
     1621 gcgagcttgg gtacttgtcg gtgtcctcaa ccgccgtcgt ttcaactttt acgtaacgta
     1681 gaaagcgatc tagcaaagtg tccacgagcg ctcctcagct tgaagcctgt tgccgccccg
     1741 atcagccgct agggggagct ccaacgccaa gccacccgag ggggccgtcg agtttgtgca
     1801 gcgttcccac agggtagctg taaagccgtc acgaatcaaa cccaacgcgg gcggccacag
     1861 ggccgtgccc ggaaattaac gccgcgcggt ggcctccagg aactgccgcc gacaccttcc
     1921 cactttccca cgttcccact gtcacggacg acccgttgtg ctgtcctcac cagccccgcc
     1981 gcatacgcca gcggctacgc cggctgcggc gtacacaacg cggatgccaa caacgacggt
     2041 tccgtcaacg cattcgacat tgatccgttc gtgctgattc tctcgggtgg ctgatcgtca
     2101 attttgcgcg aggcgatgat ggggccggcg cttcgtgtca ccggccccgc tcgctgctcc
     2161 gaccgtgttc atgtcgaatc cgagtcctaa ttcctggagg ggcgtgcagt gcgtacggaa
     2221 ccgcaggccg caagcgcttt cccccgtttc ggctccactt tggtggttga atatcaggcc
     2281 ccgacggcaa tcgcacgaca aggtcccccc gcagtgaccc tccacgcagc gcagatcgtc
     2341 agtcgtgatt gccgctaccg gctgaagcaa gcgacgaacc ggacggttcg aaactacaat
     2401 actgccggtc tggtaagcgt cggcgctttc taaagcgagc aggtaacttg cgggcgagca
     2461 gtagccgggc ccgcgaattc cgacactccc tggcccccgt tgaccagctc cctaaaccgc
     2521 aagtaaccac ggcccgtagc cgggtacttc cgcgagagcc gcttggctgg ggattcggtc
     2581 gccttaacgc gactcgatgg tcagatcgtc aatctcagta attacttcga tcattgtagt
     2641 gcggtccttc gcctagcagg tgatcgtgtc aatgaaacca gcgattgcgg cgatcacttc
     2701 gaatccggct tccaatagca tcacgaacac gtccatgatt ttctcctctg aaatgagagc
     2761 cgatggatac cgaaaacgct gcgctcggcg cttgcggcgt gctgccgacc cggtacactg
     2821 cggtcctcga agcgccgaaa gcgcctggga gtaatcagtg ccctgacgac gctaatttgg
     2881 cgcgccagga aaactcggcc acgaaaccta aaaactcctc gcttaagacg caaatgtagc
     2941 cggctcgcat tcgaatgtca atactgaaat ccagagattt ttgagagagt tcagttgacg
     3001 cacacagaag gccgcgacac tgctcttccc aacaatccgc ccgaaccggt gcgctgtgtc
     3061 cactgcggtg aatcgcggcg attagctctt gtatgcccca gggtttgccg ttgtgcagtc
     3121 tgcgagtttg ttcatccctt aagtagactt ccgatggcga ctggcgctgc ggccctgctc
     3181 gagggtttcg gtgacgtcgc gcccggaaac gtgctacgcg aacgataccg cctggtggac
     3241 gtcatcggcc gcggggccca cggtgtcacc tactttgccg agcatgaatt tctgaaccat
     3301 ccctgcgtcc ttaagattct gccgcaacgg attcgcgacg cctccgattc ggcggtccaa
     3361 cgtctccgct atgaggcgcg ggcgggattc cgagtcaaca atccgcatgt cgtgcgcgtt
     3421 ctcgattgcg acgtgatcaa cgacgtctgg tacttcgtca tggagttcgt ggatggaatt
     3481 gatctggcaa cactcatcga gaacggcgtc tcgctctgct ggcaacaggc ggcggggctg
     3541 gctatcgact ccgctaccgg gctgatggcg atccacaatg ccggattact acatcgcgac
     3601 atcaaaccgg ccaacctgct gctcggggct gatggccgcc tgcgcatctc ggatctcggc
     3661 gtggccggca tggcttcgcg ccgcgcgggt ggctcaagca gcccgggggc cgtcgggaca
     3721 ctggcctacg ccgctcccga gatgttgata ccgggagcga cggtcgacgc ccgggcggac
     3781 ctcttctcgc tcggcgccac gctctaccat gcggtgaccg gcaagcgccc cttcgccaac
     3841 gaaagcgtat tcgcgacgct catcaacacg cagacgcggg ccgtggagtg gccggagcag
     3901 gcgcccgcca atgtgccggc ctggttcatc aaaacaatcc tgacgctgct ggcgaacgac
     3961 ccggcccagc gcttcgcctc ggccgaagcg ctgcgaaacc acctggcccg gccacagcag
     4021 cagccggcta ccgtcgccgc tcccgcggtc tcgagcaacg gcgtccagcc gcgcggcttg
     4081 gccgttttgc cctttgaaaa tgaatccggt gaaggtgcgg acgactggct ggggttcgcc
     4141 ttggccgaca cgctggcccg cgagctctcg cgcatcccgg gaacgtacgt cgccgaccat
     4201 gagcagttgc gggccgtgct gcagcggccc gagatagccg ccgtgggtgg tttgaacgag
     4261 cagcttctgg cggccgggcg cgtggttggg gccggcacca tcgttaccgg ccgttttcgg
     4321 cgcgggcaag agtcgatctg tttttggtcg gacgtggtgc ggcgctccat gccgcaaccc
     4381 gagcggatcg gtcccctcca aggaccgctt tcgcgaatgg tggaactgca aagcctgctg
     4441 tttacacggc tggcccagct attgcagttg aatccgggcg cggccggcgt gggcgcggcg
     4501 ctgcccaagg tcgccctgga aacacgtgag aagttcacgc gcggcaagca ggcctacctg
     4561 caaggagatt acgagaccgc gattcgtctg gcgcgcgacg taattgaaga agacgctgac
     4621 ttcatcgaag ccctgcaata cctcagtgcg tgcagcgctc ggctaggccg atacgaggag
     4681 gcgggcaagt atcaccggca aatcacgagc ctggccgaac agcgcggcga caaacaactg
     4741 cttgtcgaag ccaaggccaa catgggagtt atgtactact tgaaggggga gtacgagcag
     4801 gcccagcggc tgtacacgga agcggcggtg atcgccgagg gactggggct cgaggcggag
     4861 cgggcgcaga tttacaacaa cgccggcttt gccttggcac gcctcgggcg cagcgaggag
     4921 gctgaggcgg cgtttcgccg ggccgtcgac gtatgcaagg gatttggagc gctggcgccg
     4981 ctcattggcc cgtacaacgg tttgggcaac ctgctgctgg atcagcagcg ctacgccgag
     5041 gcacgcgatt attatcaacg cgccttgacg ctggcccagg agatcggcga tcgcacgaac
     5101 acgggcatct cccacatgca tttagggcgc tgtgcgtcgc ttgaagggca gttcgccagc
     5161 gccaaaaacg agttcgcgct ggcgctgaac acgctggaag gcacgtcgtt ctggaacgca
     5221 cgtaccatca cctatgagta catggccgaa atgaacctgc gcctgggcga ctatgccgag
     5281 gcggtgcgct gcgcggaaaa gaagatcgag ttggcgcggc ggcacgccaa tcgcacaatg
     5341 gaggcggccg cttggcggca gaaggccgaa gctttgaggc tgtcggggcg tgcgcgtgag
     5401 gccgacgaat gtctgcttca ggcccgcgcc gcccagccga ccggatcaca gcctgcggat
     5461 tcaggagcta ccgagcccgc ttagcgggcg cttcgtgagc gagcccagct atgttcccga
     5521 tagtgctgct aatcctgctg aatgtggccg ttctgggggt aattgtgctg gtgttgctcg
     5581 agctgcgacg gttgcgcgcc gtccggaaga gcggggtcga aagccggcaa attcaacagt
     5641 tgaaggatct cttaagcgcc attcccgatc cagtgattgt gctcgattcc aaggggcggc
     5701 gagtcaagaa ccactcgggc aacgatcatg gcgaggccgc ggcgctgctg gacaagctgc
     5761 ctgagccgga gcgcaatgcg ctcaacgaga aggtagccct tgccatcaag cagcgtcagc
     5821 caggctccca gcgcatgctg ttggacgggc gttcgatcat ggcgcagatc gttccgctgc
     5881 cgaatgccca ggatgaagcg gccgtctggc tgcgcgattg caccgaccag gaggtgtccg
     5941 ccacgcgcgc cggcaagctg gccatgcgca accgcgccat tctgcggagc gccatggatg
     6001 gtttcttcgt cgtggatgag aactaccgct ttgtggaggt gaatgactcg ttctgccgca
     6061 tgaccggcta tagcccggac gagctgctgc agatgaagat caccgacctg gaggtgaagg
     6121 agccggccaa tcgccgcgcc gacgtgagcc cgatgcgcac cggtctgcac caatttgcca
     6181 cggcccaccg gcatcgtgac gggcacatcg ttcgcctgga gaccagcgtc atcgtgctgc
     6241 gcgacgatga ccacaaaatc ctggtcggct tcgcgcgcga cgtgaccgag cggctgcgcg
     6301 accaggagcg cctcgagcaa ctcagccggc agaataagca gattctggac tgcgccggag
     6361 agggcattta cggggtggac ctggacggcc ggatcagctt cgccaacccc gctgcggccc
     6421 gcatgctcgg ctgggaggta cacgagctga tcggaatgtg cgctcacgac ttgatgcgcc
     6481 gcggccaggc gcagcccgcc ccaatgctgg ccgagggctg cccgatatgc gaggccgtcc
     6541 gcgggggaat cgtccggcag ggcgtggata cgttgttccg gcgaaaagac gggacggagt
     6601 tgacggtcga tttcatcagc tcgcccattc ggcaacacgg gcagctaacc ggggcggtgg
     6661 tggtcttccg tgacaccagc gagcgcaaac gcgccgagaa cgaacggcgg caactcgaag
     6721 cccgcattca gcagagccag aagctggaaa gcctgggaat gctggccggc ggtctggcgc
     6781 atgatttcaa taatctgctg ctggccgtgc tgtgcaacgc ctcactggcc atagagcgtc
     6841 tacctccgga ctctgaggcg tgcgctttcc tgaacaaaat cgtcaaatcc gggcggcggg
     6901 cttcggagtt aacccggcaa atgctggctt acgccggccg tgcccgctac gacgtgcagc
     6961 cgctcgagtt gaattcgctc gtggaggaga tggttgacct ggtcggggcg gccctgccaa
     7021 agcaagtcaa ggtggagcta aacctggcgc cggatctgcc cttggtggaa ggtgacgcca
     7081 gccagctcca gcaaatcctg atgaacctgt tcatgaacgg ggcggagtcg ctcggatcgc
     7141 aacccggaac agtctcggcg cgcacctcgc tgcgaacact ctcggccgcg gaactggcgg
     7201 ctgactatat cggcaattcc ctcgccccgg gtgactacgt gtgcctggag gtcgccgaca
     7261 ccggatgtgg catgagcccc gccgtgctgg cgcgcatctt cgatcccttc ttcacgacca
     7321 aggccaccgg acgcggcctc gggctgtcgg cgctgctggg catcgtgcgc tcgcatagcg
     7381 gcgcggtgcg ggtcatcagc gccccaggac agggtacgac tttcacgctg ctcttcccgc
     7441 cggccgcagc ggacgccggc ctcaggcctc cacgccagcc cgtaaccgaa ctgccgcccg
     7501 gagcgaccat cctggtaatc gacgacgaag aagacatccg agaggtcgta caagcagtgc
     7561 tcgagtcgcg cggcgcacga gtcctgttgg ccaaagacgg cgccgccggc atcgagcagt
     7621 tccgccgctg cgccgcggat atctcggccg tgctgctgga catgaccatg ccgggcattg
     7681 ggggcgaggc cgtactggag gaaatcctcg ccatacgccc ggatgcgcgg gtgctgctat
     7741 ccagcggctt tggcgaggaa gaggctttgg ctcgtttacg cgacagcggc gccgcaggtt
     7801 tcgtccccaa accctacacc gcccaggcgc tcgtcgatcg catcggcgca gcccttgggg
     7861 ctcgcgccac gccgctggaa agcgctgcta cgcccgttgc ggccgacaaa cctgactcag
     7921 ggtgaagtcg cccgcccgga tttgaaatcg tccagcagtt tctcgaaatc gcctttcagt
     7981 tgcgggtcga gcacgcggat cttggcctgc tggtacagcc gcgtgatcat ttcgttcatc
     8041 ttcttccgct ccacccgggc gcgcaactgc cgctcgacct cgtcccgtac atcgtccagc
     8101 gtgacatccg ttggcggaac tcggccctcc agcttgagaa tgtgaacgta ttgaccggtt
     8161 tgcagagggg ctgatacctg gcccaccgag agtgagaagg cgtaatcgcg cagggccgcc
     8221 ggaatctccg tgtcggtgaa cgtgaaaggt tccatcatcc ccccgcgggc ggccgtgtcc
     8281 tggttgatgc tgacggcccg ggcgacctcg gcgaaatcga ctccgcggct gagttgatcg
     8341 agggcttcgt tcaacgcccg caaatcgccg gccgcgacct ggatgtgccg cacttgtacc
     8401 ttctcgccgt agagccgcgc gaactcctcg cgcaaggtgg cctcgtcgac cgtgaaatcg
     8461 gcctcgacca gcttgcgcag atgggcgttg cgctccatgg cgatcatgaa ctcgggcagc
     8521 gtcaggcagc gctgctggag aatggtctgt aacgcctgtt gcttgctttc gggcgtaagc
     8581 gtgacgcccc gggaatcgct ggtgggcacg atttcctcca gcgcccggtc gaactgggcc
     8641 tggatatccg cgggagtcac cgtcagccca cggcgctgcg actcctgttt ggccaggtca
     8701 agcgcgatca tctgctgcag caccgccagc ccgtgagttt cgatcagctt gtcgaccacc
     8761 tgcttgcgcg agatcggctg gccattgaca atggccatcg cgtcgccatc ttgcgcgcag
     8821 gccggcgcgc cccacagcag agcgaacagg ccgacggcgg gcaggcaggc ggtcaaaaag
     8881 cggccgcgaa cgacacgggg aagtcgaaac agcatgggtg gcctccaagg ctcgggggct
     8941 gcggcgcgcg ggtgcgtccg ctgcatacag gcgggcatta tagcgattag gcaatcagac
     9001 gatcacgtat taaggcgggc cgcaccggac gcactctgcc tgcgtgtagc ctcggcaacg
     9061 gcgggttata ctgcccgcct ggccccgagc acgcgatggg cgggcctgag ccgcgcagag
     9121 cgaagcttaa gagagccgaa gcgcaggaag ccggcggaga agcaacgaca aggacgtcat
     9181 gttccgcaga cgcctgacct ggttctggat tttgctgacc gccttggcct gcgcgctgat
     9241 cgcccgcctg gtcgacatcc aggcgctgca cgccgaggaa taccgcgatc tggcggcgcg
     9301 tatgttgacg caaccggtgc gttatctgcg ggccccgcgc ggcagcatcc tggaccgcaa
     9361 cgggcgcgtc ctggtcagcc atgagccatc gttcgacatc agcgcacatt tcgcggtcct
     9421 gaccgggcgg agcagcgaat acctgcacgc actggcgcgc gagctgcggc ggcgcggcga
     9481 gtatcccgaa tcgatgtcgt tggacgagat cgccgggcgg ctgcggctgc aaatcgcggc
     9541 cacttggcaa cggctctccg agctgacggg tcgtccggtc tccgagttca tcgagcgcgg
     9601 cgaggagatc cgccagcagg tggcgcgaat ccggatggca gtacggcggc ggacggggat
     9661 tgaccagccg gtggccgaag agagcgatcc gctgctggcg atgcacccgc tgattgagga
     9721 cgtcgaccac gagacggcgt tgaaggtcag gctggagctg gaagcgtacc cctggttgtc
     9781 cgtggcgccc gggtcgcggc gcgtggcgca tgatgccgat gccgccgttc atctgctggg
     9841 acgcctcggg gcggtcagcc gcgaacggca gcaggaggac ccgttggccg gcgacgagct
     9901 acggcggttg cgggccggcg agagctgcgg cattaccggc gtagagcggc tggccgaaac
     9961 cacgctgcgc ggcacgcgcg ggcgaattgt agaggagctg gacggaactt tggtggagcg
    10021 cagtgagccg gtggcgggcc aggacgtaga gctgacgatc gacatggagc tgcaggaatt
    10081 tgttctggca gccctgcggg aggcggttga ggggacggag acgacgcccg gagttcctta
    10141 cgccagcgga gcgtcggcgg tggtgctgga cgtggcgacg cgcgaggtgc tggcgctggt
    10201 gagctatccc gcttacagct acgagcgttt cgacgaagac tatgacggac tggtgcgtga
    10261 cgcgcggcga acgccgctgc gcttccgggc ggtgagtggc ctatatccgc cggggtccac
    10321 gtgcaaggcg attacgctgg tgggtgcgct gagcgagggc gagacgacgc cggaggagcg
    10381 tattcactgc accggccacc tgctgccgga gctggagaat cagttccgct gctggattta
    10441 caacgactat cgcacgacgc acgacgcccg gcagcccgag ggccagcgcg gggaggacgc
    10501 aatccggaac tcgtgcaaca tctatttcta caagctgggc gggcggctcg ggccggagcg
    10561 cctgtgcaac tggttcagca ccttcgggct aggccggttg caggggacgg ggctgatcga
    10621 ggaagcgccg ggcgtcgtgc cgaccgagcg ctggctgcgc gccaatcagc ggcgcgggtt
    10681 cgagcgctct gacccttgga actggtcgat cgggcaaggc gaggtggcgg cgacgccgct
    10741 gcaaatcgcc aacgtcggtg cgacgattgc ctcggggcac tgggccccgg tacggctgct
    10801 tcgcgacgcg ccgccggcgc ctgcggatca aatagtaaag ttgccgccag acgccttgcg
    10861 tgtgctgcgc accggcatgt ggcgggtcgt gaacgagaac ggaaccggaa aggcggccaa
    10921 gctgaagagc gaccgctacg tactgtgcgg caagaccggc tccgcccagg cggtgcccac
    10981 gccggtcagg taccggtaca tattcgagtg gccggacggg cggcgcgaaa cgcgcgaggc
    11041 gtacctggaa gaggatgcgg ccgccagttt cggagccgag cggccgcgct gcatcggccg
    11101 gcgcacgagc gcccggtttc cggaactgga agagggggag tcgatcgcgc acgcctggta
    11161 catggcctat acacagccgg cgagcacgcc gcgcggggcg gcgccgcagg ggccggtgta
    11221 cgcaatatcg acggtaatcg agttcggcgg ttcgggcggt cgcgtggccg gcccggtggt
    11281 taagaagatt gccgaatacc tgctggagcg agaccactag atgacggccc cggcatcgcc
    11341 gctcaatctg acgctgttgg gttggggcat cgccctgccg gtagcttttc tggcggcggt
    11401 ggggctggcg tgcattcacg cgactgaggc gaacgatccg gttgcgcccg tcggcctcgt
    11461 agcgacgccg gcggtcgagc cgcaagcggc ggtgctggag cgcgtgatac aagccatcgg
    11521 gccgctgacg ctacggcaga tgttctatct ggtaaccggc gtgctgctga tgctggcggc
    11581 gctagtgccg agctatcaaa agatcgggcg gtgggcatac cccgcctatg cgatactgct
    11641 ggtgctgctg gtgctgctgg tggtcgacat ttacgtggac ttgccgctga tcccggtgcg
    11701 gaacaatgcc cggcgctggt tggcctgggg gccgctgtcg gttcagccgg ccgagtttat
    11761 gaaggttgga ctgacgctga cgctggcgcg ctatctgcgt tttcgcagtt cgtaccgcag
    11821 tctgcgcggg ctgctgccgc cgcttttcct gacgctcgtg cccatggtcc tgatccttag
    11881 ggaaccggac ctgggcatga tgctgatgct gctgccggtg ctgtttctga cgctgttcgt
    11941 ggccggcgcc cggctgcggc acctggcgtt gatcattctg ctcggctgcg cgacgctgcc
    12001 ggtgtttttc atttacggga tgaagccgta tcagcgggac cgggtgctga cggtaatcaa
    12061 acagaacacg gcggacgagc gctggcatcg cgggccgggg tatcaactgc ggcagtcgaa
    12121 gattgccctg gggtcgggcg ggttgacggg cgagggttgg gggcgcggcg tgtttctgga
    12181 gtcgcgcgtc aggctgctgc cggaagagca caatgatttc attttcgcga tcatcgggca
    12241 tcagttcggc ctggtcggct gcgtgctggt gattctggcg tacggcgtca tcgtattctt
    12301 cggcatcgag gtggccacgg cgaccaacga tccgttcggc cggctgctgg cgatcggcgt
    12361 ggtggtgatg atcgttgcgc aggcgctgct gaacatcggc atgacagtcg gcctggcgcc
    12421 gattaccggc atgacgctgc cattcgtcag cgccggcggc agcagcctgt gggcgaactt
    12481 cctagcgctg ggattgctgg tcaacgtagc ccagcgccgc ccaatgctga tcgcgaaccc
    12541 gccattcgag cacgcggaag aagaatagct atcacgccat acatcgcgag ggatcgaggg
    12601 gtcgagggat cgagggatca agaagtgtaa agagcagagc cggggcgtgc cgccccggaa
    12661 t
//
LOCUS       DAOSYS010000088        12538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4236_length_12538_cov_5.077465, whole genome shotgun sequence.
ACCESSION   DAOSYS010000088 DAOSYS010000000
VERSION     DAOSYS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4236_length_12538_cov_5.077465"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLU99_07160"
     CDS             complement(<1..1607)
                     /locus_tag="PLU99_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOO16872.1"
                     /translation="MKEAAAQDTSVRPPATVQVALLERVLFYVLLGIVCARPLISESF
                     ERLAISFLPNTSGGTTPAWTTWLDSLTLIASAAILVRGWRRQRGGGWVLAGLGGLMLA
                     VALSVSAAGEQRPALNAGFSLLIAVLAGAALVQVMRAPWMPRLLLAATLASGATMATK
                     CALWKAYEHHDTMAAWAEYKPQLEAAGHDLSAPTVINFERRLQSGEAIGYQSHPNVTG
                     SFLMMWLLTAAGVCAAWLAAGRAAVGVDRSAALLFCAALSLMLGIGLWYTQSLGAFVA
                     LAGGLLLLIALGFLRRRVALRPGRALAVLVGVYLAQIAGGTTYGVAHGFRHPSLAFRW
                     QYWTAGAQVFLDAPLTGVGRLNFGDAYLQHKAAASPEEVRDPHNLWLSLLVELGPLGL
                     AAGSILLFGSITAVFRRMNGQIEGGAAQSAISVGQVTAAMLAVPLTHFIFSGRVEGSA
                     TGLLWAFEFAAVWLVCFAVITRLVDLLPDSNAARGWLLAGIAAALLAALIHGLIDFAL
                     LTVGGLYLFVTLLAAAVAFRPPPAAQPA"
     gene            complement(1604..2707)
                     /locus_tag="PLU99_07165"
     CDS             complement(1604..2707)
                     /locus_tag="PLU99_07165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOO16873.1"
                     /translation="MSRPVLLLLMSAAALGAAGLSAVLTAAAVRICPRIGFTDHPGGH
                     KSHKVPMPYGGGVAIFIAAWLPVAAAILLLRWMGPDWGIERFGESIRPYLGGLEQRSG
                     QALVLLIGAAVLHVLGLIDDVRALGAYPKLIVIAGVGLLVTVWGGLRIGAMWGDAVSI
                     PLTVAWFIVIVNAFNFLDNMDGLSSGVGWLCLVSLAACGLMAGQVFVPALACIFIGAV
                     LGFWFFNFPPARIFMGDAGSLVVGYMLATTAVMTTYFRSGMDAPPYALAMPLVILAVP
                     LYDFVSVVGIRLAEGRNPMRGDQRHFSHRLVDHGLSRRFAVLTIYLATATTGLTATLL
                     PGADLRRTLTVAFIVLMVLGIIAILESPARKTA"
     gene            complement(2704..3282)
                     /locus_tag="PLU99_07170"
     CDS             complement(2704..3282)
                     /locus_tag="PLU99_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOO16874.1"
                     /translation="MARIRAAQPVKLIVGLLGGDVDLLRRARQLLVHEYGNIDLESDF
                     WPFEETDYYAAEMGPGLQRWFLSFERLVRPEALVEIKRRTNELESRIAEQCLSPERPR
                     PVNLDPGYVSLAKLVLATTKDRDHRVYLGGGIYAEVTLHYRDKAWQAWPWTYADYRRP
                     EYMAWFERVRSVYRKQSENMTRSTQSGAGEPG"
     gene            3409..4647
                     /locus_tag="PLU99_07175"
     CDS             3409..4647
                     /locus_tag="PLU99_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOO16875.1"
                     /translation="MNIGLFEDSGCQNLLPMTWLRACFELRCGRDRLIDKLRTHWGPQ
                     ISRLWVREALRDVVAERVELDPPTPDGQWLLVNARVLLTGPAAPPASGVAWMRNGTLV
                     AAGALAADVENLTERFFCDDEAVHAWLLARGFRFESRPDTVTLIDHPWDLPLANADEI
                     RRQCAGSGGVHGGKIHPGAHLLNPAEIHIAPGAVIKPGVVLDAEQGPIHIDTAAEIQA
                     NAVIMGPAYIGPRSIVRPGAVLRGGVTIGPVCKVGGEIETSIFQGYSNKQSYGFVGHS
                     LIGEWVNLGAGTVTSNLKNTYGAIRVYINGVGVESGQHFIGSIIGDHSKTGIGTILPT
                     GCVVGIASNVFRQSAVPRFVPSFAWLTEAEMTNYRVEKALNIARIVMARRDVHLSDAE
                     AALLKSAADQAGQVEAAGWQ"
     gene            4896..6446
                     /locus_tag="PLU99_07180"
     CDS             4896..6446
                     /locus_tag="PLU99_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO16876.1"
                     /translation="MPIQHSRFTTIRSAALIAVITVSAYSLIPLSRAAEPTQSALPAQ
                     DALTPSELAHLETLSNSFRKLARLSRRGVVYIVVESAKSAEPDPDLLDYLRQRFAEVV
                     PDEQLQELARRIPGGSGTGIIFDRHGHILTNSHVVRERDEIEVRLPDERVYPAKLVGV
                     DTKTDVAVVKIDAPDLHPLTFADSDQAEVGDWVLAVGAPFGLTQTVTHGIISATGRGH
                     IRGLDSIYYQNFIQTDAAINPGNSGGPLLNMRGEVLGINTAIATDDNRNAGVAFAIPS
                     NMALKIARILIEKGVVSRGWLGISFAPAGVTSADMEILDLPHDRGVLVSSVLAKSPAL
                     AAGVQVDDYITGINGQELNGAGQFTNLMGDFLPDTQLTLQVFRDGRKQDIDIRLAQQP
                     DNPNDRSLLLDAAQGRYVPQLGLSGRALRPKTVDEFEADDRGVFVTHVDEDGPCATAV
                     RSGEVITAVNDRKIRNLAELEAALNKPALLQRQAERQDASGAGPREIRLDILQKDYDQ
                     RLETVSLP"
     gene            6791..8194
                     /locus_tag="PLU99_07185"
     CDS             6791..8194
                     /locus_tag="PLU99_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOO16877.1"
                     /translation="MSSTEEFRIEHDLLGQKKIPVGAYYGVQTARAMENFHISGVPIK
                     QYPDLIRALAVVKLAAARANYECGKISKTVLDGIDGACHEIMDGKLHDQFLVDLIQGG
                     AGTSTNMAANEVIANRALELMGHKKGEYQYCSPLDHVNCSQSTNDAYPTALHVAFIFI
                     NDRLIDELDLLVAALSRKGREFAGILKMGRTQLQDAVPMTLGQEFDAWAHSLDNEVEA
                     LRHSENVLLEVNMGGTAIGTGLNAPRGYATKCVRQLAQITGKEYRLARNLVEATQDTQ
                     PFVLYSAVLKSLAIKLSKICNDLRLLSSGPRAGINEINLPPKQPGSTIMPGKVNPVIP
                     EVVNQACYRVIGNDLAVTLAAEAGQLQLNVMEPVIAACILDSQTLFMNSARTLREHCI
                     DGITANADICRYHVEHSIGLVTALNPVIGYDTASELAAEALRTGRGIVELVREKKILT
                     DEQIADVLDPAKMTGRT"
     gene            complement(8282..10735)
                     /locus_tag="PLU99_07190"
     CDS             complement(8282..10735)
                     /locus_tag="PLU99_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16878.1"
                     /translation="MTPINATKQRGRGRSAATAGVRPGVGDGSPGAGRAAAWPSAGLG
                     AAWLRTGLPLLVAGVTFAVFLPALQNQFVSWDDYGFVVDNPHFRGFGAEQLKWMFTAY
                     LLGHYQPLTWVSYAIDYHIWGIADATGYHLTNNILHALNAVLFYFLVLRLLRLAFQVP
                     PKAASRGLYLSAALAALLFGVHPLRVESVAWVTERRDVLSVAFLLPCVLFYLRYARGE
                     SRRWLWYAGSLIFLVLSLLSKAWGITLPAVLLVLDLYPLRRLSGPKGRAAGPPVLLRL
                     ILEKIPFFLIAACFAVWAAGAQAAGHLTMLSLADHSLAQRMAQAAYGLVFYVWKTVLP
                     LGLSPLYEIPPKMNPLALRYVLSGLIVLGGVVTLVLLRKRWPGGLAVLACYVLPLAPV
                     LGFMQSGQQLVADRYSYVACMALAVLAGAAWYWVLERVRRWRWGPPIAVVVPLAPLVV
                     LGMLAWQQIAVWRDTNSLWTHAAKVDPTNRLAHYNLAVEFAKRKESERAAGLFEHVLR
                     LDPKYASAYGSLGRLRQEQGRPLEAIALFQAALDIIWNQPKIHHWLGVALQEVGRTAE
                     AARHFAEAGRLEADPRAAHISRAMALVGQGRIEQALPEFQAALDYDPNDLEARYDFGL
                     ALAKLRRFQEAVPQFELCIQSNPAHFESQNALGLALEMLGQRERAIEQYQAALRLRPE
                     AREPSVRAAKLLAQTGRASEAAALWRQVLAVDASDYETANNLAWLLATTADPAVRNGA
                     EAVRLAEAACQATQFREPVFLSTLAGAYAATGRFPEAIQILRQIIPQVEATGNQALVA
                     DLNRRLALYQAERADGDGN"
     gene            10967..11095
                     /locus_tag="PLU99_07195"
     CDS             10967..11095
                     /locus_tag="PLU99_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16879.1"
                     /translation="MPLAAGDLRTGRGIVELVREKKILTDQQIAGVLDPAKMSGGA"
     gene            complement(11183..12391)
                     /locus_tag="PLU99_07200"
     CDS             complement(11183..12391)
                     /locus_tag="PLU99_07200"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO16880.1"
                     /translation="MTVPISGSDQIVVAGSSVNLNGSASTDPDGAPLAFQWQQTAGAP
                     IVALVNAGTATASFTAPGEATTLTFQLTVNDGQGGTASDETVVTVVTTPISKPILYIA
                     NRRINSVTAYDLTTAAEIEGDLPPNANLAGPQTSLDQPTDLLIDNAGNLVVINADNNS
                     ITSYPDALDLGQINANLAPSRNVRGAATLLVHPMSMALNRAADLLFVSNEDFGTVLVY
                     AGASTAALDGNLAPLRIITSADMDGPFGIRFGANDELYVASFNLETVAVFANASTLNG
                     TVNASRIIDSPVFSRPHGVFVDENDTLYVVNSGSGAIRRNEVNVFANAATLNGPVMPD
                     STLVIPGAMNLAGIAVDSAGTGYVADTMADIIFIYDNIANRDGTIEPDRALQGPNTQM
                     GGPISVFISE"
     gene            12461..12532
                     /locus_tag="PLU99_07205"
     tRNA            12461..12532
                     /locus_tag="PLU99_07205"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12493..12495,aa:Gly,seq:ccc)
BASE COUNT         2523 a         4285 c         3705 g         2025 t
ORIGIN      
        1 gcgggctgag cggcgggcgg cggccgaaat gcaaccgccg cggccagcag cgtcacgaac
       61 agatacagac cgccgacagt aagtagcgcg aagtcgatca atccgtgaat aagcgccgcc
      121 agcaaggccg cggcaatccc ggccaacagc cagccgcgcg ccgcattgct gtcgggcagc
      181 aggtcgacca gccgcgtgat aaccgcgaaa cacaccagcc acacggccgc aaactcgaag
      241 gcccagagca gccccgtcgc cgacccctcg acgcgtcccg agaatatgaa gtgcgttagc
      301 ggaacagcga gcatggccgc ggtcacctgg ccgaccgaga ttgccgactg tgccgctcct
      361 ccctcaatct gcccattcat acggcggaac acggcagtaa tcgaaccaaa cagcagaatc
      421 gaaccggcgg cgaggcccag cggcccaagc tccaccagta acgagagcca taggttgtgc
      481 ggatcgcgga cttcttcagg actggcggcg gctttgtgct gcaggtaagc atcgccgaaa
      541 ttcaatcgcc cgacgccggt cagcggcgcg tccagaaaaa cctgcgctcc cgcggtccag
      601 tactgccatc gaaacgccag cgagggatgc cggaagccgt gagccacgcc gtacgtcgta
      661 cccccggcga tctgcgccag gtacacgccg accagcaccg ccagcgcccg ccccggccgc
      721 aacgcgaccc gccgccgcag aaaacccagc gcgatcagca acagcaaccc gccggccaaa
      781 gcgacaaacg cccccagact ctgcgtgtac caaagcccga ttcccagcat tagcgagagg
      841 gcggcgcaga agagaagcgc tgccgagcgg tcgactccca cagcggctcg tccggcggcc
      901 agccacgccg cacacactcc ggccgccgtc agcagccaca tcatcagaaa cgagccggtg
      961 acgttggggt gcgattgata tccaatggcc tcaccggact gcaggcgccg ctcaaagtta
     1021 attacggtcg gcgcgctaag atcatgcccg gccgcttcaa gctgcggctt gtattcagcc
     1081 cacgctgcca tggtgtcatg gtgctcgtag gctttccaca gcgcgcactt ggtggccatg
     1141 gtggccccgc tggccagcgt ggcggccagc agcaggcgcg gcatccacgg ggcccgcata
     1201 acctgcacca gcgccgctcc ggccaggact gcaataagca ggctgaagcc cgcgttcagg
     1261 gccggccgct gttcgcccgc cgccgaaacc gagagcgcga cggcgagcat caggcccccc
     1321 aggccggcca gcacccaacc gccgccgcgc tggcgccgcc agccgcgcac caggatcgcg
     1381 gccgacgcga tcaacgtcag cgaatccagc caggtggtcc aagccggcgt tgtcccgccc
     1441 gaagtgttgg gcaggaatga gatcgccagg cgttcgaacg actcgctgat gagcggccgg
     1501 gcgcacacga tccccagaag cacgtagaac agcacgcgtt ccagcaaggc gacctgcacg
     1561 gtggccggtg ggcgaacgga cgtgtcctgg gccgcggcct ccttcatgcc gttttccgcg
     1621 ccggagattc cagaatcgca ataatcccta gcaccatcaa cacgatgaag gctaccgtta
     1681 gcgtccgccg caggtcggcg cccggtagca aagtggcggt caagcccgta gtggccgtcg
     1741 ccaggtagat ggtcagcacg gcaaaccggc ggcttagccc gtggtcaacc agccggtgcg
     1801 aaaagtgacg ctggtcgccg cgcatcgggt tgcggccctc cgccaggcgg atgcccacga
     1861 cgcttacgaa gtcgtacaga ggcacggcca gaatgacgag cggcatggcc agggcatagg
     1921 gtggggcatc catgccgcta cggaaataag tcgtcatgac cgcggtggtg gccagcatgt
     1981 agcccacgac caggctgcct gcatcaccca tgaatatgcg ggccggcgga aagttgaaga
     2041 accagaaccc cagcaccgcc ccgatgaaaa tgcaagccag cgccggcacg aagacctgcc
     2101 cggccatcag cccacaagcg gccagcgaca ccaggcagag ccaaccaacg cccgaactca
     2161 acccgtccat gttgtccaga aaattgaagg cgttgacgat cacgatgaac caggccacgg
     2221 tcagaggaat cgagaccgca tcgccccaca tagcgccgat gcgcagccct ccccagactg
     2281 tgaccagcag gccgacgcca gcgatcacga tcaacttggg atacgccccc agtgcgcgaa
     2341 cgtcgtcgat cagacccagc acgtgcagca ccgccgcccc aatcagcaag accagcgcct
     2401 gccccgaccg ttgctccagc ccgccgagat acggccgaat gctctcgcca aaacgctcga
     2461 tgccccaatc cggccccatc cagcgcagca ggaggattgc cgccgcgacc ggcagccagg
     2521 ccgcgatgaa gattgccacg ccaccgccgt agggcatggg caccttgtgc gatttgtgcc
     2581 cgccggggtg atcagtgaac ccgatccgcg ggcaaatgcg caccgcggcg gccgtcagca
     2641 ccgcggagag cccagccgcc ccgagcgcgg cagccgacat aagcagtaga agtaccgggc
     2701 ggctcatccg ggctccccgg cgccagattg cgtggaacgc gtcatattct cactctgctt
     2761 acggtacacg ctgcggacgc gttcgaacca agccatgtac tcgggccggc gatagtcggc
     2821 gtacgtccac ggccaggcct gccaagcctt atcgcggtag tgcagcgtca cttcagcgta
     2881 aatgccgccg cccaggtaaa cccggtgatc acggtccttg gtggtagcca gcacgagctt
     2941 cgccaagctt acataaccgg gatccaggtt taccgggcgt ggcctttcgg gcgacaggca
     3001 ctgctcggcg atcctgctct ccaactcgtt cgtgcgccgc tttatctcaa ccagcgcctc
     3061 cggtcgcacc aaccgctcga agctcagaaa ccagcgttgc aggcccggac ccatttccgc
     3121 cgcgtaataa tccgtctctt caaacggcca aaaatcgctt tccaggtcga tgttcccgta
     3181 ttcgtgcacg aggagctgcc gcgcgcggcg tagcaagtcc acgtccccgc ccaaaagacc
     3241 gactatcagt ttcaccggct gggccgcccg aattctggcc atggcccgaa gtgtagcagc
     3301 gcgatagcat ttcagcgacg ggtctccccc tgtggcgccg gcgtcctgtg gcaccggcgt
     3361 ccccccggcg tatgcttatt ggggcaccgg catcccgccg gcgaggttat gaacatcggc
     3421 cttttcgaag actccggctg ccagaacctc ctgccaatga cctggctgcg cgcgtgcttc
     3481 gaactgcgct gcggacggga ccgcctgatc gacaagctgc ggacgcactg gggcccgcag
     3541 atttcgcgct tgtgggtgcg cgaggcgctg cgcgacgtgg tcgccgaacg cgtcgaactc
     3601 gacccgccca cgccagacgg gcaatggctg ctcgtcaatg cgcgcgtcct gctcaccggc
     3661 ccggccgctc cgccggccag cggcgtggcc tggatgcgga acgggacgct agtcgccgcc
     3721 ggcgcgctgg ccgccgacgt ggaaaacctc acggagcgtt ttttctgcga cgacgaagcg
     3781 gttcacgcct ggctgttggc ccgtggcttc cgcttcgagt cgcggcccga caccgtaacc
     3841 cttatcgatc acccgtggga cttgccgctg gccaacgccg acgaaatccg gcgccagtgc
     3901 gccggttcgg gcggcgttca cggcggcaaa atccatcccg gcgcgcatct gctcaacccg
     3961 gctgaaattc acatcgcccc gggagcggtc atcaagcccg gcgtggtgct ggatgccgaa
     4021 caaggcccga ttcacatcga caccgccgcc gaaatccaag ccaacgccgt catcatgggc
     4081 ccggcctaca tcggcccgcg cagcatcgtc cgcccgggtg cggtcctgcg cggtggcgtc
     4141 accatcggcc cggtctgcaa ggtgggcggc gaaatcgaga cctcgatctt tcaaggctat
     4201 agcaacaagc agtcctatgg gtttgttggg cacagcctca tcggcgaatg ggtcaatctc
     4261 ggcgccggaa cggtcaccag caacctgaaa aacacgtatg gcgccattcg cgtttacatc
     4321 aacggtgtag gggtcgaatc cggccagcac ttcatcggct cgatcatcgg cgaccattcc
     4381 aagaccggca tcggcaccat cctgccgacg ggctgcgtcg tcggcatcgc ttcaaacgtg
     4441 ttccgccaga gcgccgtgcc gcggttcgtc ccgtccttcg cctggctcac cgaggcggaa
     4501 atgacgaact accgcgtcga gaaggccctg aacatcgcgc gcatcgtcat ggcccgccgt
     4561 gacgtccatt tgagcgacgc cgaagccgcc ctgctcaaga gcgccgccga ccaagccggc
     4621 caagtcgaag ccgccggctg gcagtagccg ccccgtccag cgtaacacgc tgaaaaaccg
     4681 cgtgtgccac tgttgaagca ttaggtgtgc caccgctgaa gcaggcggca tcagcagtgc
     4741 cggtcaagaa gcagtgctca acgcatttcg cccgccgccg ccggcatcac cccctgcatc
     4801 cctctcgccc gcatcgaata taatgaacac gagggaagcg ctttccgtgc gtcaacgcac
     4861 gacgcgcccg gttacccgcc ttcgacggag aactcatgcc catacaacac tcgcgcttta
     4921 cgacgatccg cagcgccgcg ttgatcgcag ttatcactgt cagcgcctac tcgctaattc
     4981 cactttcccg cgccgccgag ccgacccagt ccgctttgcc ggcacaggac gccctcaccc
     5041 cctccgagct ggcccacctc gaaacgcttt ccaacagctt ccgtaaactt gcacgcctgt
     5101 cgcgccgcgg cgtcgtttac atcgtcgtcg aatcggccaa atccgccgaa cccgatcccg
     5161 acctgctcga ctacctacgc cagcgcttcg ccgaagtcgt accggacgag cagttgcagg
     5221 aattggcacg ccgcattcca ggcggctccg gcaccggaat catcttcgac cggcacggcc
     5281 acatcctgac caacagccac gttgtacggg aacgcgacga aatcgaggtg cgcctgcccg
     5341 acgaacgtgt ttacccggcc aaactcgtcg gcgtcgacac caaaaccgat gtcgcagtcg
     5401 tcaagatcga cgccccggac ctccaccccc tcaccttcgc cgactccgac caggctgaag
     5461 tcggcgactg ggtcctcgcc gtcggcgcac ccttcggcct cacccagacc gtcacgcacg
     5521 gcatcatcag cgccaccggc cgcggccaca ttcgcggcct cgacagtatt tactaccaga
     5581 atttcatcca gaccgacgcg gccatcaacc ccggcaactc cggcgggccc ctgctgaaca
     5641 tgcgcggtga ggtgctcggc atcaacaccg ccatcgctac tgacgacaac cgcaacgccg
     5701 gcgtggcctt cgccattccc tcgaacatgg ctctcaaaat agcccgcatt ttgatcgaaa
     5761 agggcgtcgt ctcccgcggc tggttgggaa tcagcttcgc cccggccggc gtgacctcgg
     5821 ccgacatgga aattctggat ttgccccatg atcgcggcgt actcgtgagt agcgtactgg
     5881 caaaatcgcc cgcattggcc gccggagtgc aggtggatga ctacatcacg ggcattaacg
     5941 gtcaggagtt gaacggcgcc ggccaattca cgaatctgat gggcgatttc ctgccggaca
     6001 cacagctaac tctgcaagtg ttccgcgacg gccggaaaca ggacattgac attcggcttg
     6061 cccaacagcc ggacaacccc aacgatcgtt cgttgttgct ggatgcggct cagggcaggt
     6121 acgttccgca gcttggcctg tcgggccgcg ctttgcgtcc gaagaccgtc gacgaatttg
     6181 aagccgacga ccgcggcgtg tttgtgacgc acgttgacga agacggtccg tgcgccactg
     6241 ccgtcagaag cggcgaagtc atcaccgcag ttaatgaccg caagatacgc aatctggccg
     6301 aattggaagc ggctctgaat aagccggcgt tgctgcaacg ccaagcggaa cggcaagatg
     6361 cgtctggtgc cgggccgcgt gaaatccgtc ttgacatctt gcaaaaggat tacgaccaac
     6421 gcctggagac cgtgagcctg ccctagccct cttgcgcgat ctgtgtcacc ttagcgagcc
     6481 gctattattt tgagaccaag gtcgctcaac ccagcaagac tgcttttatg ggaccatcgg
     6541 tcacaccttc cgatcaccca acctgccggc cgacgctctg cccccctgcc gtttacggcc
     6601 gcctcatcat cctggcggcc caggcactca tggccccttg acggttgcct tgtttacgcc
     6661 tgcccggata cgcggtaact tcgagcaccg tgagccggag tgtctcagtc tacggcgatg
     6721 cgggtcgtaa accgctccac ccattacgac cactcctggc tccaacacgc tcccatgagg
     6781 aaacgtagct atgtcctcca ctgaggaatt ccgaatcgag cacgatctgc tgggccagaa
     6841 gaagataccc gtaggcgcat actacggcgt acagaccgca cgcgccatgg agaacttcca
     6901 catctccggc gtgccgatca aacagtaccc cgatctcatt cgcgccctgg ccgtggtcaa
     6961 actcgccgcc gcccgcgcca actacgaatg cggcaagatt agcaagaccg tcctcgacgg
     7021 catcgacggc gcctgccacg aaatcatgga cggcaaacta cacgaccagt tcctggttga
     7081 cctcatccaa ggcggcgccg gcacgtcgac caacatggcc gccaacgagg tcatcgccaa
     7141 ccgcgccctc gagttgatgg gacacaaaaa aggcgagtac caatactgca gcccgctcga
     7201 ccacgtgaat tgctcgcaat cgaccaacga cgcctacccc accgcactgc acgtggcctt
     7261 catcttcatc aacgaccggc tgatcgacga acttgatctg cttgtcgccg ccctcagccg
     7321 caaaggccgc gaattcgcgg gcatcctcaa gatgggccgc acccagctcc aggacgccgt
     7381 ccccatgacc cttggccagg agttcgacgc ctgggcccat agcctcgaca atgaagtgga
     7441 agccctgcgc cacagcgaaa acgttctgct cgaggtcaac atgggcggga ccgccatcgg
     7501 cacgggtctc aacgccccgc gcggctacgc gacgaagtgc gttcgtcagc tcgcccagat
     7561 caccggcaaa gagtaccgcc tcgctcgaaa cctggttgaa gcgacccagg acacgcagcc
     7621 gttcgtcctc tactccgccg tactcaagag cctggcgatc aagctttcaa agatttgcaa
     7681 tgatctgcgg ctgctttcgt cggggccgcg cgccggtata aacgaaatca acctgccccc
     7741 caaacagccc ggctcgacca ttatgccggg caaggtcaac ccggtcattc cggaggtcgt
     7801 gaaccaagcc tgctatcgcg tcatcggcaa cgacctggcg gtgacgctcg cggccgaggc
     7861 cggccaactc caactgaacg tcatggagcc ggtgatcgcg gcgtgtattc tggactcgca
     7921 gacgctgttc atgaactcgg ctcgcacgct gcgcgaacac tgcattgacg gcatcaccgc
     7981 caatgccgac atttgccgct atcacgtgga acacagcatc ggcctggtaa ctgcattgaa
     8041 cccggtgatc ggctacgaca ccgcgagcga actcgcggcc gaggccctgc gaacgggtcg
     8101 cggcatcgta gaactcgtgc gcgaaaagaa aattcttacg gacgaacaga tcgccgacgt
     8161 gctggacccg gcgaagatga ccggtcgtac gtaggccgcg agaggctcga tagcgccttt
     8221 gtcgtgcaag gcggtgccgc ggcggcgtcg agcgcgacgc gcagttcaga tgactctaca
     8281 gctaattgcc gtccccgtcc gcgcgttcgg cctggtagag cgccaagcgg cgattgaggt
     8341 cggcgactaa cgcctggttg cccgtggctt caacctgcgg aatgatctgg cgcagaattt
     8401 gaatcgcctc tggaaagcgc cccgtggcgg cgtacgcacc ggccagcgtg ctcagaaaaa
     8461 caggctcgcg aaactgcgtc gcctggcacg cggcctccgc caaccgaacg gcctccgcac
     8521 cgttgcggac agccggatcg gcagtcgtgg ccagcaacca tgccaggttg tttgccgtct
     8581 cgtagtcact tgcgtcaacc gccaacacct ggcgccacag cgccgcggct tctgaggccc
     8641 gcccggtttg cgccagcagc ttggccgctc tcaccgacgg ctcccgcgcc tcggggcgca
     8701 gccgcaacgc cgcctggtac tgctcgatcg cgcgctcgcg ctgccccagc atttcaagcg
     8761 ccagccccag agcgttctgc gattcgaagt gcgccggatt gctctgtata caaagctcga
     8821 actgcggcac cgcttcctgg aagcgcctca gcttggcgag cgctagcccg aagtcgtagc
     8881 gcgcttcaag atcgttcgga tcgtagtcga gcgctgcttg gaactccggc aacgcctgct
     8941 caatccgccc ctgcccaacc agcgccatgg cgcgcgaaat gtgggccgcg cggggatccg
     9001 cctccagtct tccggcctcg gcgaaatgcc gggcggcttc tgctgtgcgc cccacctcct
     9061 gcagcgccac gcccagccaa tggtggatct tcggctgatt ccagatgatg tccagcgccg
     9121 cctggaagag cgcgatcgct tcgagcggcc gtccttgctc ttgccgcaag cgtcccaggc
     9181 tgccgtacgc gctggcgtat ttcggatcga gccgcaacac gtgctcgaac aggccggccg
     9241 cgcgctcact ttccttacgt ttggcaaact ccactgccag gttgtagtgc gccagtcggt
     9301 tcgtagggtc gactttcgcg gcgtgcgtcc aaagcgaatt ggtgtcgcgc cagacggcaa
     9361 tctgctgcca ggcgagcatt cccaagacca caagcggcgc caaaggaacc accacggcaa
     9421 tcggggggcc ccacctccag cggcgaacgc gctccagaac ccagtaccaa gccgcgccgg
     9481 ccagtacagc cagcgccatg caggcgacgt aggagtagcg atccgccact aactgctgcc
     9541 cgctctgcat gaaccccagc accggcgcca acggcaacac gtaacacgcc agcactgcca
     9601 gtccccccgg ccagcgcttt cgcagcagga ccagcgtaac aacgccgccc agcacaatca
     9661 ggccggatag aacatagcgc aaggccagcg ggttcatctt cggcggaatt tcgtacagcg
     9721 gcgagagacc gagcggcagg acggtcttcc acacgtagaa aaccaggccg taggcggcct
     9781 gcgccatgcg ttgcgcaagc gaatgatccg ccaacgacag catcgtgaga tgtccggccg
     9841 cctgcgcccc ggcggcccac actgcaaagc acgcggcgat caggaagaac ggtatttttt
     9901 ccaagatcag ccgcagcaac accggcggcc ccgccgcgcg ccccttcggc ccgctcagac
     9961 ggcgtagcgg gtaaaggtcg agcaccagca gcaccgcggg cagcgtgatg ccccacgcct
    10021 tggacagcag cgagagtacc aggaatatca gggagcccgc gtaccaaagc cagcggcgcg
    10081 attcgccgcg cgcgtaacgc aggtagaaca gcacgcaggg gagcaagaat gctaccgaca
    10141 gcacgtcacg gcgttcggta acccatgcga ctgattcgac ccgcagcgga tgcaccccga
    10201 acagcagtgc cgccaatgca gcggataagt agagcccgcg cgaagctgcc ttgggcggca
    10261 cttgaaaggc cagccgaagc agacgcagta ccaggaaata gaaaaggact gcattcaagg
    10321 cgtgcagaat gttgttcgtg aggtggtaac cggtcgcgtc ggcgatgccc caaatgtggt
    10381 aatcaatggc gtagctgacc cacgtcaacg gctggtaatg gcccagcaag tacgcggtga
    10441 acatccattt gagctgctcg gcaccgaacc cgcgaaagtg cgggttgtca accacgaagc
    10501 cgtagtcgtc ccaactgacg aactggttct gcagcgctgg gaggaagacc gcgaatgtca
    10561 cgccggctac cagcaatggt agtccggtgc gcagccaggc tgcgcctagc ccggcgctcg
    10621 gccacgccgc ggctcgcccc gcgcccgggc tgccatcccc aacacccgga cgaactcccg
    10681 cggtcgccgc ggaacggcca cgtccacgtt gcttggtggc attgattggc gtcatcgatc
    10741 gcacccgtga ttcggcgcag cgcaatacgc ggcggatgcc agcgattaca agagcgcctc
    10801 aatcttacca cacgccacaa gcccgcaccc gccaaaaaag ccagctaagc cgcgcagcac
    10861 aaaccgctcg aatcgccggc gcgcaaccgc gaacagatcg cgtcgtcgcg tcgcccgccc
    10921 gacgtcaatg cctggcgacc ggccggccaa gttttagctc cgtatattgc ctctcgcggc
    10981 cggggacctg cgaacgggtc gcggcatcgt ggaactcgtg cgcgaaaaga agattcttac
    11041 ggaccaacag atcgccggcg tgctggaccc ggccaagatg tccggcggtg catagccact
    11101 aagccgcgac gttccgcccc accggcagct cgacgcccgg cggtcgtgtt caggccgaac
    11161 tctgccacgc cgcgttctgt cgttactcgc tgataaagac gctgatcggc ccccccattt
    11221 gtgtattggg gccctgcaac gcgcggtccg gctcaatcgt cccgtcacga ttcgcgatgt
    11281 tgtcgtaaat gaaaataatg tcggccatgg tatcagcgac ataaccggtc ccggccgagt
    11341 ctaccgcaat gccggccaag ttcatcgcac cgggtataac cagcgttgaa tcaggcatga
    11401 ctggtccatt gagcgtggcg gcattcgcga agacattgac ttcgttccgc cggatggcgc
    11461 cggagcctga attcaccacg tacagagtgt cattctcgtc aacgaacacg ccgtgcggcc
    11521 tggagaacac cggactatcg atgatccgcg atgcgttcac ggttccgttg agcgtgctgg
    11581 cattggcgaa gaccgcgaca gtctctaagt taaaactcgc cacatacaat tcgtcattgg
    11641 ccccgaacct tattccgaag ggcccgtcca tgtccgcact ggtaataatg cgcagcggag
    11701 ccaggttgcc atcgagggcc gccgtcgagg ccccggcata cacgagaaca gtcccaaaat
    11761 cctcattgga tacaaacagc aggtcggccg cccggttaag ggccatcgac atggggtgca
    11821 ccagcagcgt cgccgccccg cgtacgttgc gggagggagc gaggttcgcg ttgatttgac
    11881 cgagatcgag ggcgtcaggg tagctcgtaa ttgagttgtt gtcggcattg ataaccacca
    11941 agttgccggc gttgtcaata agcagatcgg tcggctggtc cagcgaggtt tgaggtccgg
    12001 ccaagttggc atttggcggg agatcgccct cgatttccgc cgctgtcgtg aggtcgtagg
    12061 ctgtcacgct gttgatcctg cggttggcga tatacaaaat gggcttggag ataggggtcg
    12121 tgactacagt cacgaccgtc tcgtcggagg ccgtgccgcc ctgtccgtcg ttcaccgtaa
    12181 gctggaacgt gagcgtcgtg gcttcgccgg gagctgtaaa cgacgccgtg gcggtgccgg
    12241 cgtttaccaa tgcgacgatt ggcgcgcccg ccgtctgctg ccactggaag gcgagcgggg
    12301 ccccgtccgg gtcggtgctg gcgctgccgt ttaagttgac agagctgccg gcgacgacaa
    12361 tctggtcgga gccggagatc gggactgtca tggcatgcag cgggcggcaa cgggccttga
    12421 cggcgcggcg taacccccga taatgacgag agataaggct gcgggcgtaa ttcagtggta
    12481 gaatgccagc ttcccaagct ggacgtcgtg ggttcgaatc ccatcgcccg ctttttct
//
LOCUS       DAOSYS010000089        12451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4278_length_12451_cov_5.571717, whole genome shotgun sequence.
ACCESSION   DAOSYS010000089 DAOSYS010000000
VERSION     DAOSYS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4278_length_12451_cov_5.571717"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PLU99_07210"
     CDS             <1..313
                     /locus_tag="PLU99_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOO16881.1"
                     /translation="VAKPPVMTEKRVVEATTTPKPDTKATRTYVVASGDTLTTIARNV
                     LGDGSRWRQIYELNKDKIKNPDVLLEGTELKLPDNGTKPPTAAPKPGNGAKKTAPKPK
                     P"
     gene            complement(351..1574)
                     /locus_tag="PLU99_07215"
     CDS             complement(351..1574)
                     /locus_tag="PLU99_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16882.1"
                     /translation="MNRIALFAIPLTLALVFGCPPRGGDENLNQNENANDNAVNDNSA
                     ANVNNAADENANVNFAVENDNESESGYVAALAPAVWRRTSSQSMRGGGQTDLDYLVLN
                     DDSTADLHFRHPDLGFLLCYDGLFMTTPGVVLLYVSDLLPGGLMAQYELLDDNTLRLF
                     SPEGQSLEFERVAELPDEYRCQDVTVVNTFDELPSPQDQTGLAYDGTHFWYTHWGSGA
                     SELQPIDRTTGVAEAPVAAPTPPYVHACQGTHFWFCSGATQIVVRRSPDGTPQDEVDT
                     NADLGTPTEIDVLTYDSDAHRLWLYGRAAATGTYRLLRVESDAEPDVLADVYPLDIDM
                     AAMAWHDGLLWILLDTNPPVVITFDPESLTCPNTYALPPGETNWFGLAWAGDSLYLIG
                     KEAGDGVLLQAQVSP"
     gene            1869..2813
                     /locus_tag="PLU99_07220"
     CDS             1869..2813
                     /locus_tag="PLU99_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOO16883.1"
                     /translation="MSRFWDRIFGTVERQDWLDRVGDPLEAALKKTFDAGGPVGKKAG
                     DFLHGTWFGHPLHAALTDVPVGAWTTALVLDVLDDTISRRRGYSTAARHALAVGIGGA
                     VAAAVTGLADFQHLSVKQRRIGLTHAMMNTGALVLMTSSYMARRRGNIDTGKALTRLG
                     WAVMAMAAHFGGHLVYQQRVGVTKADERGRPGEFLPVLPVGELREDDPRRVIVDDVRV
                     VLVRHAGRIYALGEECSHMGGPLAEGEIVGDSIKCPWHGSHFALADGRVEQGPAIHAQ
                     PCFEIRIRDGQIELRRAERGRELPAYASALPAGAPGAS"
     gene            complement(2938..5685)
                     /locus_tag="PLU99_07225"
     CDS             complement(2938..5685)
                     /locus_tag="PLU99_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO16884.1"
                     /translation="MSNKSVCVRKSVRVVACAALLAIFVSGAAAGPPNYMIINSPTFT
                     GSAGLNSLVAAKTAQGFNVTIKEFPTTVIPETIRAYIQQFWNTPDAPDYILIVGDSNL
                     IPAWYGQGTRHANTDLYYACMDGPDDWYPDVPIGRYPVRTTAQLTAVVNKTNYVEAGS
                     FADPSYITRVAFLATDDPGAMAPDSHDAMIEAYTTPAGLTPTRIYFPYPSGTGTPEIT
                     AAVNAGSLIVTYMGHSGATSWWGPSFNQNNVNALTNSNKYCLVLGLSCDTADFTATEC
                     FGETWLRKSNGGAVAFISASNRIFYTTEQEWESVRHLENYLFRAFFEDGEYRVGPALQ
                     RALYYFLADPDYGPTHEYTLNYFEEFTVLGDPSLQMPILGFQIDVDPPLQSRCAPATQ
                     VQYTIQVEPHAGFNQAVTLAASGYPAGWNVSFSQNSQPPPFTSVMTVSNISGAAGMYE
                     ILLTGTSSGGLHRYALPKLHLASGVPGAVVLSDPPAGATGVSRSPVLAWQAAGQAAQY
                     ELQIASDYAFTDVVYSATTDLTSHNVDDYLLQLTDYYWRVRGINGCGAGPYSAIRSFT
                     TVPPPNYFTEQFPAGVPLDLQYHTYYFIPNGSADFYEVCGEEATALPTNPTGGIWLEL
                     YEDSSEVVYPLRPISLYGNSYSVVFVNSNGNLTFVNGDSTAAETFAVHFNQPRVSPLF
                     TDLSAQLGSVTYKNLADRLAITYQNVPERYSSSGNTFQVEFFYDTGEIHITWLQCPAD
                     GAIVGLSAGQGVPADFTPSDFTDYPCAAACCHADATCTVTEQAACVAPDLWHPEWPDC
                     AVADCQLPTGACCHEDGTCEVTEEIACTSPSVWHAEWADCSVAECPQPAVQRGDADCD
                     GTVNVFDIDPFVMALTDLPAYTAQYGCAANADCNCDGAVNVFDIDAFVSCMIGECPDC
                     P"
     gene            complement(6114..6998)
                     /locus_tag="PLU99_07230"
     CDS             complement(6114..6998)
                     /locus_tag="PLU99_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO16885.1"
                     /translation="MSDNERWLVTGASGQLGGHLLRQLKRDKVDKTLLALAGRGPVDE
                     NVKTLRVDLADEQGLRRAVDDFRPTHVVHAGAITAVSDAYQRPQDAERVNTYATRVLA
                     DAAELCAARFVFISTDMVFDGTAAPYRESDPPRPLSHYGRTKAAAELLLVGRTHVLTI
                     RLPLMYGLPCGGRETTFSRQLAAARRGEPLKLFVDEWRTPVWIQDAARAVIGLARTGL
                     TGLIHVAGPERLSRFDLIERIVAAMGIADAKLQPVSRLSIAAPEPRPADLSLDCGLLR
                     SSFPELAPRPVAACGPGF"
     gene            7129..9339
                     /locus_tag="PLU99_07235"
     CDS             7129..9339
                     /locus_tag="PLU99_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16886.1"
                     /translation="MKRVGHGSAAILFVIISLVPATRGADERSSTPLREASSDNRRFQ
                     LRIRPGRPGEDAIRGCKAGLYERVGDRRGGRALWERFLVNDVAPGRAFIRDDGRFVVT
                     LDEYRRGGARNAMVIYGENGELLRHFLLPDLLDRSDWAEVKKNGEAVDWLDGARFQFL
                     DEPPMFVVTLRHGGEIRVDLEKLQVVRKSGAVSGVSAIPREMLTALYGPNARPASQPA
                     GAETVRPRPEKRRQPDVAVAITKGDGEGPQTDVAQISEPEDLPVSDEVAAMEGAEAAD
                     EIAGEAVESEDSQTMDEEDAGFAVAEEEADPLDQMAEEVRAETSVEASKWGGVPQPDP
                     AAPVDYLAWARSFITIEGPSAVPEYEAIMASVAPWKGSRESLKAALAGDPSVLGTPDI
                     RAWLADNQDALDHFRKALTLEYKGWPLKSGDGSFIAALLPNLSPVREVSCVSVVQGRL
                     HAAAGLADSAAECYLDALAGGAQAGSGFTLIESLVGAGIQKLAAEALLDLQADPAGEQ
                     LDYVALAERLEEDYRSPSSPSETLQAERAFFLDTVQSMYTYDSESGTYTLDAAKATEF
                     LHTIESPPREDVAARVERLHQIDFKSSVADGNDYYDKLGQALALPYPEAQQMLEALER
                     RFQEPNKSNPLLIPAAYSRYHTLRAGADALRRATRLVTHLNAYRQQYGDYPESLEVFG
                     GADFTVDPFTGDNLKYERTGGTFRLYSVGPNGADEGGVHDPRGENNDIVYWPRP"
     gene            complement(9349..10326)
                     /gene="ribF"
                     /locus_tag="PLU99_07240"
     CDS             complement(9349..10326)
                     /gene="ribF"
                     /locus_tag="PLU99_07240"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOO16887.1"
                     /translation="MEVLHSLTEYLPPAGGVALTIGNFDGVHLGHRKIIQRVLHIAEQ
                     RGLPSVVITFDPHPVAVLAPERAPARLVNLPEKLHLLGELQVTACIVLRTERRLLALE
                     PEEFLNVLVRHCRPRAVVEGLDFNFGHGRRGSVQTLREFADKLGYTVEVVETARCEEI
                     PGAPSVHSSAVRAALREGRVDLARAMLGRPYRVTGVVGSGAGRGARLGFPTANLHQIE
                     HMLPQEAVYAAAAQLADGSLYLAAVNVGPQPTFADMQARVEAHLLDWHGVLNGQPMAL
                     YFLQRLRAQTRFGSTAELCTQIAQDVAATRTHADVLNELRSKPLMPLFR"
     gene            complement(10437..>10761)
                     /locus_tag="PLU99_07245"
     CDS             complement(10437..>10761)
                     /locus_tag="PLU99_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16888.1"
                     /translation="DQGRPAPADLPLPEEPEPANLTPAEREQFEQGIIDRRSRYIYLA
                     SHRAQAAAGRPALVCQDVQMHDLYIRPDQLHVILFDGQRYELLTLHREDLPRMADKRG
                     WNPPK"
     assembly_gap    10762..10861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10862..11149)
                     /locus_tag="PLU99_07250"
     CDS             complement(<10862..11149)
                     /locus_tag="PLU99_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16889.1"
                     /translation="MRESSAAGAAPSAQKRMQLEILKRRRRRVWRNALFILLVTLVMA
                     LLSVAERDNQSLRGSVQRCHDDLAHACRALQTAFDQGRPAPADLPLPEEPEP"
     gene            11316..11456
                     /locus_tag="PLU99_07255"
     CDS             11316..11456
                     /locus_tag="PLU99_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16890.1"
                     /translation="MNVAEILWVVAGAVLTAVIAAAVGFWTVREARDAARDNFADSRK
                     DQ"
     gene            complement(11450..>12451)
                     /locus_tag="PLU99_07260"
     CDS             complement(11450..>12451)
                     /locus_tag="PLU99_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO16891.1"
                     /translation="AIALDEEDETLLANLALVREKLHDYDAAIELYDRALELGADLDW
                     VLVRKAAALTEAGDTEAAKVMLKRYLSLVPDDAEQLVALAILYSDDEQYEQAYAAYRE
                     AEQIDRNSGGLRLNWGVTAVRAGDLDQARAQLEHLERLESGSARPLLLRAFICEEEHE
                     LAAARRCYADALARVQPSNTEELVYALEMAMDFSARNGMRANCEKLMRRAYAANACTA
                     ELCEPYREVNGDLLADGYWFSMVVEADFRLGLFAVHESGPDGAGRPRRYQRRFQVIAR
                     NHDEAIDLVTRFAQRAGERHVVVREFLREEKIQDLYSGIYEIDAESFVLRSAPDQPH"
BASE COUNT         2357 a         3729 c         4043 g         2222 t
ORIGIN      
        1 agttgccaag ccgccggtga tgactgaaaa acgggttgtt gaagcgacta ccacccccaa
       61 gcccgatact aaggccacga gaacctatgt ggtggcttcc ggcgatacgc tgacgactat
      121 cgcacgcaat gtcctgggcg acggctcgcg ctggcgccaa atctatgagt tgaacaagga
      181 caagatcaag aacccggacg ttctgctcga gggcactgag ctgaagctgc cggacaacgg
      241 taccaagccg cccactgcag cgcccaagcc gggcaacggc gcgaagaaaa cggccccgaa
      301 accgaagccg taatcttcct ctttcaccga acgagccgca gatttccggg ctacggcgac
      361 acctgcgctt gcaggagcac accgtccccg gcttctttac cgatcaagta gagcgaatcg
      421 ccggcccatg ccagaccgaa ccaattcgtc tcgccggggg gtagggcata ggtgttcggg
      481 caggtaagcg attcgggatc gaaggtaatg acgaccggcg gattggtatc cagcagtatc
      541 cagaggaggc cgtcatgcca agccatggcg gccatgtcaa tgtcgagcgg atagacgtca
      601 gcaagtacat cgggctcggc gtcactttcg acccttagca ggcggtacgt gcctgtggcg
      661 gcggcccgcc cgtacagcca gaggcgatgg gcgtcggagt cgtacgtcag cacatctatt
      721 tcagtgggcg taccgaggtc cgcattggta tccacctcat cctgcggcgt gccgtccggg
      781 gatcgccgga cgacgatttg agtcgccccc gaacagaacc agaagtgcgt gccctggcag
      841 gcatgcacgt agggcggcgt cggagcggca accggggcct ctgccacgcc ggtggtgcgg
      901 tcgatcggct gaagttctga ggctccagaa ccccaatgcg tataccagaa atgcgtaccg
      961 tcgtaggcca agccggtttg gtcttgcgga ctcggcagtt cgtcgaaggt gttcacgacc
     1021 gtcacatcct ggcagcgata ttcgtcgggt agctccgcga cacgctcgaa ctccagggac
     1081 tgtccttcgg gcgagaacaa gcgaagggtg ttgtcatcca agagttcgta ttgggccatc
     1141 aggccgccgg gcaagagatc gcttacgtac agcaggacga cacccggagt cgtcatgaac
     1201 aggccgtcgt aacagagcag aaagccgagg tccggatgtc gaaagtgcag atcggcagtg
     1261 gaatcgtcat tcaagaccag gtaatcaaga tcggtttggc cgccgccgcg catcgactgg
     1321 gaggacgtgc ggcgccagac cgcaggcgcg agcgcggcta catatccgct ttcgctctca
     1381 ttgtcgtttt cgacagcaaa attcacgttc gcattctcgt cagcggcgtt gttgacgttg
     1441 gctgccgagt tgtcgtttac ggcgttgtcg tttgcgtttt cattctggtt taagttctcg
     1501 tcgccgcccc ttggcggaca gccaaagacc aacgcgagcg tgagcggaat tgcgaacagg
     1561 gcgatccggt tcataagagg ctcctttgcc gcggctgaac acgcgcggcg acataaccgc
     1621 cataaggccg aggccataac ccgatgttct gcgcacgtgg aacgcgccgg ccgattctgc
     1681 ctgaagaaac tttgaatctg catgcaaagt acctgaagcg actgaaatgt caaagctttt
     1741 tttgtggaca cgactctgaa tagcgcgcaa gtccaggtgt ttacacaact tgtaaacggt
     1801 ttgccgataa catcatgcgt ggcgccggca gaacccgcgt tttcgaggag acggtcatga
     1861 gaaagttgat gagcagattt tgggatcgaa tatttggaac agtcgaacgc caggactggc
     1921 tggaccgcgt cggtgaccct ctcgaagcgg cgctcaagaa gaccttcgac gccggcgggc
     1981 cggtggggaa gaaagcaggc gacttcctgc acggtacctg gtttggtcac cctttgcatg
     2041 cggcactcac ggacgttcct gttggcgctt ggaccacggc gctggtactg gacgtgttgg
     2101 acgacaccat atcgcgtcgc agaggttaca gtacggcggc caggcacgcg ctcgcggtgg
     2161 gtattggcgg cgccgtggcg gcggcggtca ccggtctggc agacttccag cacctctcgg
     2221 tcaagcagag gcgcatcggg ctgacgcatg ccatgatgaa tacgggtgct ttggtgctga
     2281 tgacctcgtc atacatggct cgcagacggg gcaacattga cacaggcaag gcgctcaccc
     2341 ggctgggctg ggcggtgatg gccatggccg cacatttcgg cgggcacctg gtttaccagc
     2401 agcgtgtcgg cgtgacgaag gcggacgagc gcggtcgacc cggcgaattc ctgccggttc
     2461 tgccggtcgg agagttgcgt gaggacgatc cacgccgcgt gattgttgac gatgtgcggg
     2521 tggttctggt ccggcatgcg gggcggatct acgcgctggg tgaagaatgc tcgcacatgg
     2581 gcgggccgct ggcagaaggt gagatcgtcg gcgacagtat caagtgcccc tggcatggct
     2641 cgcacttcgc gctggcggat ggacgagtgg agcaaggacc ggcgatccac gctcaaccgt
     2701 gcttcgaaat ccgcatccgc gacggacaga tcgagctgcg gcgggcggag cgcggccggg
     2761 aactgccggc gtacgccagc gcgctgcccg ccggcgctcc gggagcgtcg tagcggctaa
     2821 aaagacggcg gcggggtccc agccttcggt tggctagcga ccccgccgca gtgtgatcag
     2881 gcagttttca tcggcgccgg ccgagcgccg gccggcggtg cgggttcagc cccgcgatca
     2941 cgggcaatcc ggacattcgc cgatcataca cgagacaaac gcatcgatgt cgaagacatt
     3001 cacagcgccg tcgcagttgc agtccgcgtt ggctgcacat ccgtattggg ccgtgtaggc
     3061 cggcagatca gtcagggcca ttacgaacgg gtcgatatcg aacacgttga cagtgccgtc
     3121 gcagtccgcg tctccgcgct gtacggccgg ttgcgggcat tcggcaacac tacaatccgc
     3181 ccactcggca tgccagacgc tggggctggt gcacgcgatc tcttcggtta cctcgcacgt
     3241 gccgtcctcg tggcagcagg cgcctgtcgg tagctggcag tcggccacgg cacagtccgg
     3301 ccattccggg tgccagagat cgggggcgac gcacgcggct tgttcggtca cggtgcacgt
     3361 ggcgtccgca tggcaacacg cggcggcgca gggatagtct gtgaagtcgc tcggggtgaa
     3421 gtcggccggg acgccctgtc cggcggacag accgacgata gcgccgtcgg cggggcattg
     3481 gagccacgta atgtggattt cgccggtgtc gtagaagaac tcgacctgga aggtgttccc
     3541 ggagctgcta tagcgctcgg ggacattctg gtaggtgatc gccaggcggt ccgccaaatt
     3601 cttgtaggtt acgctgccga gctgggcgct cagatcggtg aacagcggtg agacgcgcgg
     3661 ctggttgaaa tgaaccgcga aggtttcggc ggcggtcgag tcgccgttaa cgaaagtaag
     3721 gttgccgttg gagttcacga aaacaacgga gtaagagttg ccgtacaacg agatcggccg
     3781 caaaggatac accacttcgg aactgtcttc gtagagctcg agccaaattc caccggtggg
     3841 gttggtgggc agcgcagttg cctcctcgcc acagacctcg tagaagtcgg cgctaccgtt
     3901 cgggatgaag tagtaggtgt ggtattggag gtccagcggt acgccggccg ggaactgttc
     3961 ggtgaagtag ttgggcggcg gtaccgtagt gaagctgcga atagcggaat aggggcccgc
     4021 gccgcagcca ttgattccgc ggacacgcca gtagtagtcc gtgagctgga gcaggtaatc
     4081 gtcgacatta tgactggtca ggtcggtagt cgccgagtat accacgtcgg tgaaagcata
     4141 gtccgaggca atctgcagct cgtactgcgc cgcctgcccc gcggcttgcc aggcaagtac
     4201 cgggctccgc gacactcccg tggcaccggc cggcggatcg ctcagcacga ccgcgcccgg
     4261 aacgcccgag gccaggtgca acttgggaag ggcgtaacgg tgcaggcccc cagacgaggt
     4321 gcccgtcaag aggatttcgt acatgccggc cgcgccgctg atgttgctga cggtcatcac
     4381 cgaagtgaac ggcggcggtt gcgagttctg gctgaagctg acattccagc cggccgggta
     4441 gccggaggcg gccagcgtca cggcctggtt gaaaccggca tgcggttcga cctggatagt
     4501 gtattgtacc tgagtggccg gcgcgcatcg ggactgcagc ggcggatcaa cgtcgatctg
     4561 gaagccgagg atgggcatct gcagcgacgg gtcgcccagc acggtaaact cctcaaagta
     4621 attgagggtg tattcgtggg tggggccgta gtccggatcg gcgaggaagt aatacaaggc
     4681 cctctgcaac gccggtccca cgcgatactc gccatcctcg aagaacgcgc ggaagaggta
     4741 gttttccagg tgccgaaccg actcccactc ctgttcggtc gtgtaaaaaa tccggttact
     4801 cgccgaaatg aacgcgaccg cgccgccgtt gctctttcgc agccaggtct cgccgaaaca
     4861 ctctgtcgcc gtgaaatccg cggtgtcaca agagagcccc agaacgaggc agtacttgtt
     4921 gctgttggtc agggcgttca cgttattctg gttgaaggac ggcccccacc aggacgtcgc
     4981 cccgctgtgg cccatgtacg tgacgatcaa gctgcccgcg ttgacggccg cagtgatctc
     5041 gggggtaccg gtgccgctcg gataggggaa atagatgcgt gtcggcgtga ggccggccgg
     5101 cgtggtataa gcctcgatca tcgcgtcgtg cgaatcgggc gccatcgcac ccgggtcgtc
     5161 agtcgccagg aaggcgaccc gcgttatata gctcggatcc gcgaagctgc cggcctctac
     5221 ataattggtc ttgttcacga cggccgtgag ctgcgccgta gtgcgcaccg gatagcgtcc
     5281 aatgggtacg tcgggatacc agtcatcggg gccgtccatg caggcgtagt acaagtccgt
     5341 attcgcatgg cgtgtgccct gcccatacca ggctgggatc aggttggaat cacccacaat
     5401 caggatgtag tcgggggcgt cgggcgtatt ccaaaactgt tggatgtagg ctctaatcgt
     5461 ctcagggatc acggtcgtgg ggaattcctt gatcgtgaca ttgaacccct gggcagtctt
     5521 ggctgccacc aggctgttca ggccggcgct gccggtgaac gtcggggaat tgatgatcat
     5581 gtaattcggg gggccggcgg ccgccccgga aacgaatatc gccagaaggg ccgcgcaggc
     5641 cacgactcta accgatttgc gcacgcacac agatttattc gacattgaaa tctcctagcc
     5701 cctcgttagc ctggaaaaag gaactttgcc attcaccctt ccaactcgag cgcagtgtgt
     5761 ggcacgaact cttgcccggc ctgtaggacg ggcaacgtcg aatgcgctca agccaaaatc
     5821 agaataacac gaaggcccgc ctgagtgcaa tactgagtta caggtctgga aaatcgtcgg
     5881 ttcgctcact tagccgcatt cgcacctgcc cagagcgcaa gattgagcga ccatccctga
     5941 taacagcctc gagatccgat aataacgagg gtgctgaaga gccaaaaaca gactgagcat
     6001 tcgcatcccg gtcgacaaac ggcgcttctg tatcggctac aactggcttg acggccagac
     6061 gttccaatgt gactggctgg gcgggctaga gaccgtgcgg cggccggcgc aggtcaaaag
     6121 cccgggccac aggccgcaac cggccgcggc gccaactccg gaaaactgct ccgcaagagg
     6181 ccgcaatcga gtgacaaatc tgccggccgc ggttccgggg ccgcgatcga gagccgcgag
     6241 actggttgca gcttggcgtc agctattccc atcgccgcga cgatcctttc gatcaagtcg
     6301 aagcgcgaca gcctctccgg gccggcgacg tgaatcagcc cggtaaggcc ggtgcgcgcc
     6361 aggccgatta cggcccgggc tgcatcctga atccaaacag gcgttcgcca ttcatccaca
     6421 aaaagcttca gcggttcgcc gcgccgcgct gccgccaact ggcgcgagaa cgtcgtttcc
     6481 cgcccgccgc aggggaggcc gtacatcaga ggcaagcgaa tagtaagcac gtgggtgcgg
     6541 ccaaccagca ggagttcggc ggcggccttg gtacgcccat agtggctgag cggtcgcggc
     6601 ggatcagatt cgcggtaggg ggcggcggtc ccgtcgaaga ccatgtcggt agagatgaac
     6661 acgaagcgcg cggcgcagag ttcggcggcg tcggccaaca cacgcgtcgc gtaggtgtta
     6721 acgcgttcgg cgtcctgggg gcgttgataa gcgtcgctga cggcggttat cgcgcccgcg
     6781 tgtactacgt gcgtagggcg aaaatcatcg accgcgcgcc gcagcccttg ctcgtcagcc
     6841 aggtccacgc gcagggtctt cacgttctca tcaacggggc cgcgcccggc cagggccagc
     6901 aacgtcttat caactttgtc gcgcttcagt tgtcgcagca ggtgtccgcc gagctgcccg
     6961 gatgcccccg tgaccagcca acgttcgttg tcgctcattt gaggctgccc acctcctttg
     7021 tcgtaggtta ccatgtcggc aaatcgaccg gggctgtggc ggccgattcg tgccgccacg
     7081 aggtacgcgg gggatcggag cgccccggat ctggcggagg atactgagat gaagcgcgtt
     7141 ggtcatgggt cggcggcgat attattcgtg atcatcagtc tggtgccggc cacgcgcgga
     7201 gctgacgagc gaagctccac tcctcttcgt gaggccagtt ccgataaccg tcgttttcaa
     7261 ttgcgaattc gcccgggacg gcccggagaa gacgctattc ggggctgtaa ggccgggctg
     7321 tacgagcgtg tcggcgaccg gcgcggcggg cgggcgctgt gggagcgctt tctggtcaac
     7381 gacgtcgctc ccggccgggc gtttatccgc gatgatggac gtttcgtagt cacgcttgac
     7441 gagtaccggc ggggtggcgc ccgcaacgcc atggtgattt atggcgaaaa tggcgaattg
     7501 ctgcggcatt tcctgctccc cgatttgctc gatcgatcgg attgggcgga agtgaagaag
     7561 aacggggagg cggttgactg gcttgatggt gcgcgcttcc agtttcttga tgagccaccc
     7621 atgtttgtgg tcacgctgcg ccatggcggg gagataaggg ttgacttgga gaagctgcag
     7681 gtagtgcgga aatcgggagc ggtctcgggc gtcagcgcga taccgcgtga gatgcttacg
     7741 gcgctatacg ggccgaacgc ccggccggcc tcccagcctg cgggcgcgga gactgtcagg
     7801 ccgagaccag agaaaaggcg gcaaccagac gtagccgtgg cgattaccaa aggcgacggc
     7861 gaggggcctc aaacagacgt ggcgcaaatc tcggaacctg aagatctgcc ggtttcggac
     7921 gaggtggcgg ctatggaggg cgcggaggcc gcggatgaaa ttgccgggga ggcggtcgag
     7981 tccgaagact cgcaaacaat ggatgaggaa gacgctggat ttgcggtcgc ggaagaagag
     8041 gccgacccgc tagaccaaat ggcagaggag gtccgtgcgg aaacttccgt ggaggcctct
     8101 aaatggggcg gcgtcccaca gcccgatccc gcggctcctg tggattatct ggcgtgggcc
     8161 agatccttca tcaccatcga aggcccgagc gccgtgcctg aatacgaggc gatcatggca
     8221 agcgtggcgc catggaaagg gtcccgggaa tcgctgaaag cagcgctggc cggtgacccg
     8281 agcgttctcg gtacgccgga cattcgggcc tggctcgctg acaatcagga cgcgctggac
     8341 catttccgca aagcactaac gctggaatat aagggctggc ccctcaaaag cggggacgga
     8401 agcttcatcg cggccttgct gccgaacctc agcccggtgc gcgaagttag ctgcgtctca
     8461 gtggtgcaag gtcgcctgca cgcggcggcc ggcttggccg acagtgcggc cgagtgctat
     8521 ctggacgctc tggcgggcgg agcgcaggcg ggcagcggct tcacgttaat cgagagcctg
     8581 gtgggggccg gcattcagaa gctggccgcc gaggcgctcc tggacctgca agctgatccg
     8641 gcgggcgagc agctcgatta tgtggcgctt gcggagcgcc ttgaggaaga ctaccgcagc
     8701 ccgagctcgc cgtccgagac acttcaggca gagcgagctt tcttcctgga tacagtacag
     8761 agcatgtaca cctacgattc agaaagcggt acgtacactc tggatgcggc caaggcgacc
     8821 gagttcttgc acacgatcga gtcacccccg cgagaagatg tcgccgcccg agttgagcgc
     8881 ctgcaccaga tcgacttcaa atcgtctgtg gcggacggca acgactacta cgacaagttg
     8941 gggcaagccc tggcactacc gtacccagag gctcagcaaa tgctggaagc actggagcgg
     9001 cgcttccagg aaccaaacaa aagcaatccc ttgctgatac cggccgccta ttcccgctat
     9061 cacacgctcc gcgcaggagc cgatgcgctg cggcgggcga cgcgcctggt cacgcatctg
     9121 aatgcctatc ggcagcaata cggggattac ccggaatcgc tggaggtttt tggcggcgcc
     9181 gatttcaccg tcgatccgtt tactggggac aacctcaaat atgagcggac cggcgggacg
     9241 ttccggctgt actccgtggg gcccaacggc gctgacgaag gcggtgtgca cgacccgcgc
     9301 ggtgagaaca acgacattgt ttactggccg cggccttgat ccggcgaact accgaaacag
     9361 cggcattagt ggtttggacc gcagttcgtt cagcacatcc gcatgcgttc gcgtcgcagc
     9421 tacgtcctgc gcgatttgag tgcaaagctc tgcggtgctg ccaaagcgcg tctgagcccg
     9481 cagtcgctgg aggaagtaga gggccatggg ctgaccgttt agcacgccgt gccagtccag
     9541 caggtgcgcc tccacgcggg cctgcatgtc cgcaaatgtg ggttgcggac ccacgttgac
     9601 cgcggccagg tacaagctgc cgtcagccaa ctgggcggcc gcggcgtaga ccgcctcctg
     9661 cggcagcatg tgctctatct ggtgtagatt ggcggtcgga aagcccagcc gcgctccgcg
     9721 gcccgccccc gacccgacca cgcccgtcac gcgatatggc cggcccagca tggcccgggc
     9781 cagatccacg cgcccttccc gcagtgccgc ccgcacggcc gaactatgca ccgacggcgc
     9841 tcccggaatt tcttcgcaac gggccgtctc taccacttcg accgtgtagc ctagcttgtc
     9901 ggcgaactct ctcagcgtct ggaccgaacc cctgcgacca tgcccgaagt tgaagtccag
     9961 cccttcaacc actgcccgcg ggcggcagtg ccgaaccagc acgttcaaaa actcctcagg
    10021 ctccagcgcc agcaaacgcc gttcggtccg gagaacgatg caggccgtga cctgcaactc
    10081 acccagcagg tgcagctttt ccgggagatt gaccagacgc gccggggcgc gttccggggc
    10141 aagcacggcc accgggtgcg gatcgaacgt aatcacaacg ctcggcaggc cgcgctgctc
    10201 cgcgatgtgc agcacgcgct ggataatttt gcgatggccc agatgcacgc cgtcaaagtt
    10261 gccgattgtc agcgctaccc cgccggcggg ggggaggtac tcggtcagag agtgtaacac
    10321 ttccattcgg aacgcctcgg caatgctgcc gtccaacttc ggccgcacca cggcgggcaa
    10381 accgggcggc cgacgcgtaa aacgtcgatg ttgcccacga cgcgctggct gcgattctat
    10441 ttcggcggat tccagccgcg cttgtccgcc atgcgtggca gatcttcgcg gtgcagcgtc
    10501 agtaattcat agcgctgccc gtcgaacagg ataacgtgca attgatccgg gcggatgtaa
    10561 aggtcgtgca tctgaacgtc ctggcacacc agggccggcc ggcccgcggc ggcctgtgcc
    10621 cgatggctgg ccaggtagat atagcgtgac cggcgatcaa tgattccctg ctcaaactgc
    10681 tcacgttcgg cgggggtcaa gttcgcgggt tcgggctcct ccggcagcgg tagatcggcc
    10741 ggagccggac ggccctgatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 ngggttcggg ctcctccggc agcggtagat cggccggagc cggacggccc tgatcgaagg
    10921 ccgtctgcag cgcacggcag gcgtgtgcca ggtcgtcgtg gcagcgctgc accgagccgc
    10981 gcagcgactg attgtcgcgt tcggccaccg agagcagcgc catgacaagc gtgaccagca
    11041 ggatgaacag cgcgttacgc cacacccgcc ggcggcggcg cttcaagatc tcgagctgca
    11101 tgcgcttctg cgcactcggg gcagcgccgg ccgcgcttga ctcacgcatg ctggtccttc
    11161 ctgctgagcg cgagccgcgc ggcgagaggt ccggccgcct tgagccggca ccgcgaagcc
    11221 attcgttgtc taccctggac gctcattggg cgtgcattct atgatggagc tccggccggt
    11281 gaaagcatga aggcctggta cggatgccgc ttgctgtgaa cgttgctgaa attctctggg
    11341 tcgtggcggg ggccgttctg acagccgtaa tcgccgcggc agttggtttt tggaccgtgc
    11401 gcgaggcgcg tgacgccgcc cgggacaatt tcgccgattc tcgcaaggat cagtgaggct
    11461 ggtccggcgc cgagcggagc acgaaactct ccgcgtcgat ttcgtatatc cccgaataga
    11521 ggtcttgaat cttctcctcg cgcaggaatt cccgcacgac cacgtgccgc tcgccggctc
    11581 gctgcgcgaa acgcgtgacc aggtcgatcg cctcgtcgtg gttgcgggcg atcacctgga
    11641 agcggcgctg gtaacggcgt ggacggccgg cgccgtccgg accgctttcg tgcaccgcaa
    11701 acaaccccag ccgaaaatcc gcttcgacca ccatgctgaa ccaatagccg tccgcgagga
    11761 ggtcgccgtt cacctcgcga tatggctcgc acagctcggc cgtgcaggcg ttggccgcat
    11821 aagcgcggcg cattagcttc tcacagttgg cccgcatccc gttgcgggcc gagaagtcca
    11881 tggccatctc aagcgcgtac accagctctt cagtgttgga gggctgcacc cgcgccaggg
    11941 cgtcggcata acaacgccgc gccgcggcca gctcgtgctc ttcctcgcag atgaacgccc
    12001 gcagcagcag cgggcgggcc gaaccgcttt ccagccgttc caagtgttcc aactgtgccc
    12061 gcgcctgatc gaggtcgccc gcacgcaccg ccgtgacgcc ccagttcaaa cgcaagccgc
    12121 ccgaattacg gtcgatctgc tcggcctcac gatacgccgc gtaagcctgc tcgtactgct
    12181 catcatcgct gtacaggatg gcgagcgcca cgagttgctc ggcgtcatcc gggaccagtg
    12241 agagataacg cttgagcatg accttggcag cttccgtgtc gccggcttcg gtcagggcgg
    12301 ccgccttgcg caccagcacc cagtccaggt cggccccgag ttccagggcg cgatcgtaga
    12361 gctcaatcgc cgcgtcgtaa tcgtgcagct tctcccgaac gagcgccagg ttggccagca
    12421 gcgtctcatc ctcctcgtcc aacgcaatgg c
//
LOCUS       DAOSYS010000090        12445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4280_length_12445_cov_5.357627, whole genome shotgun sequence.
ACCESSION   DAOSYS010000090 DAOSYS010000000
VERSION     DAOSYS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12445
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4280_length_12445_cov_5.357627"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLU99_07265"
     CDS             complement(<1..568)
                     /locus_tag="PLU99_07265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO16892.1"
                     /translation="MFPQTIGPATWAEIQRTEQFVRGRTDARAIPRQSAEFLHTLILA
                     AGCRSGIEVGTGYGYSALWIGSALARQAGALLSIERDELKVARARETFARAELDQTIR
                     VLHGRATDVLKDLSGPFDFVFLDADKASTPACFEALWPRLGHNAVIVTDNIVSHADEL
                     GEFVAHLRRHPNLCSMLVDIGSGLELSVK"
     gene            1114..2694
                     /locus_tag="PLU99_07270"
     CDS             1114..2694
                     /locus_tag="PLU99_07270"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOO16893.1"
                     /translation="MSDQISDRKVKRDKIRALGSDPYGERFTGLRHHADIRAAAEKLN
                     IEPGQILEQPDAEFRAAGRVVLYRDIGSLIFMTVRDLSGDLQYGISKKMIGQAWPVAK
                     LIDLGDVVGGQGKLGRTKTGELTLWLSDFKLLSKAVRPPPEKWHGLTDVELRYRKRYV
                     DLFANPESREIFVKRSRIIDYARKVLQQRGFIEVETPVLQPIYGGAAARPFRTHHNTL
                     DLDLYLRISPELYLKRCLVGGLERVFELARCFRNEGISPRHNPEFTMLELYQAYGDYH
                     DMMEICEAIIGGAARDVIGATRIKFGQHEIDYTTPWRRARYADLLREYAGLDMNDRPA
                     VLAKAQEIGLLAKLQKAQAALEQGVDPAKLSAPGMEQQPTPTPAQPGAAGAEFHVDHV
                     LLVNALFEELVEQHLINPTFVLDYPAPLCPLTKRHPDDPSLALRFELYINGMEMGNAY
                     SELNDPDVQRENLAGQIEGEGDETMRVMDEDFVESLEYGMPPAGGLGIGIDRMVMLLT
                     GSTSIRDVILFPLQRPRE"
     gene            complement(2709..2930)
                     /locus_tag="PLU99_07275"
     CDS             complement(2709..2930)
                     /locus_tag="PLU99_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOO16894.1"
                     /translation="MLNEFTIIFERGENGWWISTIPEVPGAFSQGRTKDEARQNALDP
                     LTELMAARRELALRQRAADSEIETIRIAR"
     gene            complement(2986..3795)
                     /locus_tag="PLU99_07280"
     CDS             complement(2986..3795)
                     /locus_tag="PLU99_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16895.1"
                     /translation="MLALFGFTLRQIFGQRKLWLAVALLLLPAAITVLIWAVGGEQGL
                     KHRWEAYHVLMQMLIGLLVPLVCMLYGNALLGAEIEQRTIVYLLTRRLHRATVLLVRF
                     AAVWLALTVLCELALLAVHLCATMSSEAVASPASRGSWQPWAAWRVYAGVTALGVAGY
                     LAVFTTISVVFARSLIVSVVYFVLFELIIANLPLTASAASITHHLRQTVMDRMPEVRG
                     LYDWPREVREQVFPTGETGIDNLLLAVAVLLGLASVLVTFRELVPARVSRD"
     gene            complement(3807..4733)
                     /locus_tag="PLU99_07285"
     CDS             complement(3807..4733)
                     /locus_tag="PLU99_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16896.1"
                     /translation="MAVIELHGLSKWYGEVIGLNNVTAAIGHGITGLLGPNGAGKTTL
                     MGLATGQLRPSQGRIRVLGQRVWDNPRLLARLGYCPEGDPFWGAMTGWRYVLFLARLS
                     GLRGAAAGAAAGTAIERVGMQEHMHRAIRGYSKGMRQRIKIAQALVHRPQLLILDEPL
                     TGADPVSRHELTELLRALAAEGVDILVSSHVLHEVEALTRQILLIDHGRIVAEGELGA
                     VRQQIHDRPHTIRIRVDDPRRLAAGVVPLPAVTGVKLLDGQTLAVETRAPDEVYAFVN
                     GAAAEGRVNVQEIAAADESLEAVFGYLTEHRL"
     gene            complement(4756..5769)
                     /locus_tag="PLU99_07290"
     CDS             complement(4756..5769)
                     /locus_tag="PLU99_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOO16897.1"
                     /translation="MSVTPAGTVHSAHYQRFRTRPRTSRWVWVAMVERGVRLALKDHR
                     NLILLMAAPGFIFGAVVIFYVLSLLEQLAGTRDAQGIYNFVKIFLRVDISGVAKLSDY
                     RLPLWEGVFVLMTRLQLFYLLIVISRLGPGLIADDLKTNALPIYFARPITPMTYLLGK
                     WLTISVFIALCMLVPNLLALGGGILVAGTPGTWVQTFGLAGNLTLIGLGVMLIGGLLI
                     LALSSLTADKRFVVVAWIAIALLPEIIQQILFEELEGASTTRFLGSLSLNNNIMLLTN
                     WVFDLRSAWQATGLPREAYEAALGRAVEPLYPALVLLAVTLTAGAIAYRRVVRFSQSA
                     ANL"
     gene            complement(5766..6665)
                     /locus_tag="PLU99_07295"
     CDS             complement(5766..6665)
                     /locus_tag="PLU99_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO16898.1"
                     /translation="MKRYGRVTALNRLSLELEAGLIGLLGPNGAGKSTLISILLGQTP
                     ATSGRAEVLGLDVHRRRRRVRQRAGFMPENDCLIGGSSGTGYVYYTARLAGLSHGDAM
                     QRAHEVLDYVGLDEARYRPAEQYSTGMKQRLKLAQAIVHDPDVLFLDEPTNGMDPQGR
                     QAMLGLISDLGRAGISVLLSSHLLPDVERVCERVVILGGGEMLASGSIAEMRRPHPTL
                     YTLDHSGDGQHLVKELQQRRVAVHNQSNGVLAIELPGAGQERLVFEAARQAGATVRGL
                     HPKRSSLEEMFMTAIRKQEQNRR"
     gene            complement(6935..8635)
                     /locus_tag="PLU99_07300"
     CDS             complement(6935..8635)
                     /locus_tag="PLU99_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOO16899.1"
                     /translation="MRIPRIGIVSRTYRHINRYRQILTVLVKYGFGGLVDRLRISQYL
                     ETGLHLLSREKPAQVEMLTPAERVRLVLEELGPTFIKLGQVLSTRPDVLPPEFIEELT
                     KLQQHVPPFAFEQVRETIETELGRPLEGLYAEFAEYPIAAGSIAQVHRATLVSGEQVV
                     VKVQRPNIRSMIAVDLEILLHLATLVERHVEGWRVYHPSRMVEEFSRVLERELDFTVE
                     AAHIDRFARQFAQDPTIYIPKIYRELSSRRVLTLEYVDVIPATDTTALRAAGLDPKVI
                     AARGAELTLKQIFVHGFFHADPHPGNAFVLPGNVICLLDFGMMGRIDRQTREQVADLI
                     YTVATRDAARVCRSLLRLAKPPDDVEPDLRAMERDVAEFIDVNIPVALGDLQFGRLVH
                     ELMRLIRQYELSVPPDLVTMLKAAATVERLVARLDSDLNMMAVAEPYIRRLKLQRLKP
                     KRFLADVYESTEELWHLAREVPSGLRDLLRLAKRGALKINFEHRGLEKLMDTHERVAN
                     RVSFAIVVAALVVGSSLIVHSRLPPTWHDIPLIGLIGYLAAGVLGFMLLVAIIRHGRL
                     "
     gene            complement(8638..8943)
                     /locus_tag="PLU99_07305"
     CDS             complement(8638..8943)
                     /locus_tag="PLU99_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16900.1"
                     /translation="MIDLVKKTFLAGIGAAAMTMDRVEQMARELANSAQLSSEKGQAF
                     VDEAVARAQKARADMQANVQRMVNDALARTNVATREDIAQLVARIEKLERDLAAKSH"
     gene            complement(9057..10463)
                     /locus_tag="PLU99_07310"
     CDS             complement(9057..10463)
                     /locus_tag="PLU99_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16901.1"
                     /translation="MRRIAYFLQTGGLSLMTRRNYMAELRHLALWGFVTGGVEGTIAS
                     VVAAKTFAAPGLLTSVIFAIPIVVNVFNLAWGLLLRGQQRIKTYTWLASGALVLVCSI
                     AFTPAGRPWSAWLFAAQIAGTHFFLSGMLTIRTTMWGANYSRICRAQVAGRLHMLNVL
                     LSVFTTALLSILFDRNPDYYRLVYPATVTLGALSLLPLRRMRVRRERAEVRRFRALAA
                     AQQNGEGSSARLWHGLKEAAAILRTDQRFADYMRAMILLGSANFFTDPVLVAILAQRF
                     GYWSSTFLIYQLPILVVLVSISFWSKFFDRVGVLRFRVSNSLVWASSYAGLIVAMAMF
                     ALDGRFFYIAGIGVLVVARVLNGLARGGGDLAWNIGHLHFASEHKIELYMGIHLGLTG
                     LRGATMPILGWLANQYLHWGSFAISLGLALSSFVLFRRMARTEGPAAPVEPVLNRSDR
                     LKAAAPETATTAVTTNSR"
     gene            10724..11032
                     /locus_tag="PLU99_07315"
     CDS             10724..11032
                     /locus_tag="PLU99_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOO16902.1"
                     /translation="MILAWLDHWTILLVGLVALLLFGSRLPEVARSLGRSLNEFKRGL
                     NEINDEIDRAPKDPNQPKLRSPSEPRLDSPAPEPRAEATPAQNKAQYPEAPPEGPAQS
                     "
     gene            complement(11076..12251)
                     /locus_tag="PLU99_07320"
     CDS             complement(11076..12251)
                     /locus_tag="PLU99_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009761095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOO16903.1"
                     /translation="MQAPDGSGRFFIVDQIGVVRVLDANGALLATPFLDVRDRMVQLN
                     AGGDERGLLGLAFHPDYANNGRFFIYYSAPRGADVPAGFNHETHVSEFRVSADANVAD
                     PASERVLLRIPQPQSNHNGGDLAFGPDGFLYISTGDGGGGGDVGFGHTPGLGNAQDIA
                     SLLGKILRIDVNSGDPYGIPPDNPLVNVDAARPEIWAYGLRNPYRFSIDTQSGQTRLF
                     AGDAGQALREEIDLIESGGNYGWNIREGTLCFDPSSPGNPPATCATTGARGEPLLNPI
                     LEYPHTDAAGNPFGSAVIGGYVYRGSAIGGLAGRYILGDFSAGSVGGKVFAATEAADG
                     TWSFSEVAISNGTNGRLGRFVKGFGRDSAGEVYVLTSLVAGPSGDTGEVLKLVAADQ"
BASE COUNT         2466 a         4139 c         3716 g         2124 t
ORIGIN      
        1 ttttgacgga cagttccagg cccgagccga tgtccacgag catgctgcac aaattcgggt
       61 gccggcgcag gtgcgctacg aattcgccca gctcgtcggc gtgcgacacg atgttgtcgg
      121 tgacaatcac cgcgttatga ccgagccgcg gccagagtgc ttcaaagcag gccggcgtgc
      181 tcgccttgtc ggcatccaga aacacgaagt caaatgggcc gctcaggtcc ttcagcacgt
      241 cggtggcgcg gccgtgtaga acgcgaatag tctggtccag ttcggcgcgg gcgaaagtct
      301 cgcgggcccg ggccaccttg agttcgtcgc gctcgattga cagcagcgcg ccggcctgcc
      361 gcgccaacgc cgaaccgatc cacaacgcgc tatatccgta gccggtgccc acctcgatac
      421 cgctacgaca tccggcggcg aggatcaacg tgtgcaggaa ctcggccgat tgccgcggga
      481 tagcgcgcgc atccgtccgg ccgcgtacga actgctcggt gcgttgaatt tcggcccagg
      541 ttgccggtcc gatcgtctgc gggaacattc cgactccgtt tgaaaacgcc gtagggtgcg
      601 gtcattgaac gccgcatggt gcggtcaccc gctcaaaacg ccgcacgatg cggtcaccgg
      661 attctatgtc cggccggaaa ggggtttacc aatgagccgc tttggtctcg cgggcgggcg
      721 ctcttgctgg gcgttggcgg gagcaaaggg aattaccggg aagccgcttt gccccatgcc
      781 tcgggtaacg tgcgcaaaaa aacagatgga aggtctagcc gtgacgcact tccgaccttc
      841 catcttgtgc ccgcgacgcg ctacaggcgc cgccggcggt tcgtacgggg cgttcacacg
      901 cttcgcacgt ctacatctat agcaagcttg cgcaaaattt gcagcagcgc gcgcggaaaa
      961 attttcaaaa atctgttcat cgcgcgcggc cggcgtcaaa cgtgcgcgac gccgcgatcg
     1021 cacctagcct ccgcgcaaaa cgcgcttgtg ggccactctt tgaatagtcc gccgtatgtt
     1081 ccgccgtgcg ccacacgcta aactaagcgc actatgagcg accagatcag cgatcgcaaa
     1141 gttaagcgcg acaagattcg cgcgctcggt tccgacccgt acggcgagcg ttttaccggc
     1201 ctgaggcatc atgccgacat ccgcgccgcc gccgaaaaac ttaatattga gcccggccag
     1261 attctcgagc agcccgatgc cgaattccgc gccgccggcc gcgttgtgct ctatcgcgac
     1321 atcggcagct tgatcttcat gacggtgcgc gacctgagcg gcgacctgca atacggcatc
     1381 agcaaaaaga tgatcggcca agcctggccc gtcgccaagc tcatcgattt gggtgatgtc
     1441 gtcggcggcc aggggaagct gggccgcacc aaaaccggcg aactcacgct ctggctgtcc
     1501 gacttcaagc tgctgagcaa ggccgtccgc ccaccgcccg agaagtggca cggactgact
     1561 gacgtcgagt tgcggtaccg caagcgctat gtcgatctgt tcgcgaatcc tgagtcgcgc
     1621 gagatcttcg tcaaacgcag ccggattatc gattacgcac ggaaggtgct gcagcagcgc
     1681 gggtttatcg aggtcgaaac gccggtgctc cagccgattt atggcggagc ggcggcgcgg
     1741 ccgttccgca cgcaccacaa cacgctggat ttggacttgt acctgcgcat ttcgccggag
     1801 ttgtatctga agcgctgcct ggtcggcgga ctggagcgcg tattcgagct ggcgcgctgc
     1861 ttccgcaacg aaggcatcag cccgcgccac aatcccgagt tcacgatgct ggagctgtac
     1921 caggcgtacg gcgattacca cgacatgatg gaaatttgcg aagccatcat cggcggcgcg
     1981 gcccgcgatg tgatcggcgc gacgcgcatc aaattcggcc agcacgaaat tgattacacg
     2041 acgccctggc gcagggcccg ttatgccgac ctgctgcgcg agtacgccgg cctcgacatg
     2101 aacgaccggc cagccgtgct cgccaaagcg caggaaatcg gcctcctggc caagttacag
     2161 aaggctcaag cggcattgga acagggcgtc gatccggcca agctcagcgc ccccggcatg
     2221 gagcagcagc ccacgccgac tccggctcaa ccaggcgcgg ccggcgcgga gttccatgtt
     2281 gaccacgtcc tgctcgtgaa cgcgctgttt gaggaactgg tcgaacagca tctaatcaac
     2341 ccgaccttcg tcctcgacta ccctgccccg ctctgcccgc tcacgaagcg ccaccccgac
     2401 gacccgagtc tggcgttgcg ctttgagctg tacatcaacg gcatggagat gggcaacgcc
     2461 tacagcgagc tcaacgaccc ggacgtgcaa cgcgagaacc tggccggcca gatcgaaggc
     2521 gagggcgacg aaacgatgcg cgtcatggac gaggatttcg tcgaatcgct cgaatacggc
     2581 atgcccccgg ccggcggctt gggcatcggt attgaccgca tggtcatgct gctgaccggc
     2641 agcacgagca ttcgcgatgt gattctgttt ccgttgcagc ggccgcggga gtgagaggcg
     2701 cgcgagcgct agcgggcgat ccgaatcgtc tcaatctcgg aatcagccgc gcgttgccgc
     2761 aacgctagct cacgcctcgc cgccatcaat tccgtgagtg gatccaaagc gttctggcgt
     2821 gcttcgtcct tggtccgccc ctgcgagaaa gcacccggca cctccgggat cgtcgaaatc
     2881 caccagccgt tttcgccgcg ctcgaagatg atggtaaatt cgttgagcat cactcaggat
     2941 tgtatttaag cttgggcata tcactcatat tctcccctcc cgccttcaat cacgactcac
     3001 gcgcgccgga accagctccc ggaaggtcac tagtacactc gcgagcccta gcagaactgc
     3061 caccgcgagc agcaagttat cgatccccgt ttctcccgtg gggaagacct gctcgcgcac
     3121 ttcccggggc cagtcgtaca gcccgcgcac ttcgggcatc cggtccataa ccgtttgacg
     3181 cagatgatgc gtgatgctcg ccgctgaggc cgtcagcggt agattcgcga ttatcaactc
     3241 gaaaaggaca aagtagacca ccgatacgat cagagaacgg gcgaagacta cgctgatcgt
     3301 cgtgaacacc gccagatatc cagcgacgcc gagtgccgtc accccggcat agacgcgcca
     3361 cgcggcccac ggctgccatg agcctctcga agccggcgac gcgacggcct cgctgctcat
     3421 ggtcgcacac aggtgaacgg cgaggagggc cagttcgcat agaaccgtca gcgccagcca
     3481 gaccgcggca aaacgtacta gcagcaccgt ggcccggtgc agccgccgcg tgagcaggta
     3541 cacgatcgtg cgctgctcta tttcagcgcc gagcagcgcg ttgccgtaga gcatgcagac
     3601 cagcggcaca agcagcccaa tcagcatctg catcagaacg tggtacgcct cccaccggtg
     3661 cttgagcccc tgttcgccgc cgaccgccca gatcaagacc gtaatcgctg ccggcaacag
     3721 caacagcgcc accgccaacc acagctttcg ctgcccgaaa atctgccgca gcgtaaaccc
     3781 aaataacgcc agcatcgccg tcatcgctat agccggtgct cggtcaagta accaaacacc
     3841 gcctccaggc tttcgtcggc cgcggcaatc tcctgcacgt tcacgcgccc ttcagccgcg
     3901 gcgccgttta caaacgcata cacctcgtcc ggcgcgcggg tctccacagc cagtgtctgg
     3961 ccgtcgagga gcttgactcc ggtcaccgcc ggcaacggca ccacgccggc cgccagtcgg
     4021 cgcgggtcgt ccacgcgaat acggatcgtg tgcggccggt cgtggatctg ttgccgcacg
     4081 gcccccagtt cgccctcggc cacaatgcgc ccgtgatcaa tcagtagaat ctgtcgcgta
     4141 agcgcctcca cctcgtgcag cacgtggctg gaaaccagga tatcgacgcc ctccgccgcc
     4201 agagcgcgca gcaactccgt tagctcgtga cgcgaaaccg gatcggcccc ggtcagcggc
     4261 tcgtccaaaa tcagcagttg cggtcgatgg accagggcct gcgctatctt gatgcgctgc
     4321 cgcatcccct tggaatagcc gcggatcgcc cggtgcatgt gctcctgcat gccgacgcgc
     4381 tcgatggccg tgccggcggc ggcgccggcg gccgcgccgc gcaatccgga gaggcgcgcc
     4441 agaaagagca catagcgcca gcccgtcata gcgccccaaa aggggtcgcc ctcgggacag
     4501 tagcccaggc gcgccagcaa gcgcgggtta tcccatacgc gctgacccaa gacgcgaata
     4561 cgcccctggc tcgggcgaag ctggccggtc gccaacccca tcagcgtggt cttgccggct
     4621 ccgttcggtc cgagcaagcc ggttatgccg tggccaatgg cggcggtaac gttgttgagg
     4681 ccgatgacct cgccgtacca cttcgacaga ccatgtagct cgatcaccgc catgcccaag
     4741 tcccggtgtg ttgcattaca ggttcgcggc cgactgagaa aaacgcacga cgcggcgata
     4801 cgcgatcgcg cccgccgtga gcgtaacggc cagcagcacc agggcgggat agagtggctc
     4861 aaccgcccgc cccaaagccg cctcatacgc ctcgcggggc agccccgtcg cctgccaggc
     4921 cgagcgcagg tcaaaaaccc agttcgtcag aagcatgatg ttgttgttca gcgacaagct
     4981 tcccaagaac ctcgtggtgc tcgcgccttc caactcctca aacagaattt gttgaatgat
     5041 ttcgggcagc agtgcaatcg cgatccacgc caccactacg aaacgcttgt ccgcggtcag
     5101 gctggagagc gcgaggatca gcagaccgcc aatcagcatc acgcccaatc caatcagcgt
     5161 caggttgccg gccagcccga acgtctgcac ccacgttccc ggcgttccgg ccaccaggat
     5221 gccgccgccg agcgccagca aattcggtac tagcatgcac agagcgatga agacgctgat
     5281 cgtcagccac ttgcccagca ggtacgtcat aggcgtgatc ggccgtgcga agtagatcgg
     5341 cagcgcgttg gtcttcaggt cgtccgcgat aagcccgggc ccgagccggc tgatgacaat
     5401 cagcaggtag aaaagttgca gcctggtcat caaaacgaac acgccttccc aaagcggaag
     5461 cctgtaatca ctcagcttgg cgaccccgct gatatccacg cgcaagaaga tcttgacgaa
     5521 gttgtatatg ccctgggcgt cgcgcgtccc ggccagttgc tccagtagcg acagtacgta
     5581 aaagatgacc accgcaccga aaatgaaccc cggggcggcc atcagcagga tcaggttgcg
     5641 atgatccttc agcgccagcc gcacgccgcg ctcgaccatc gccacccaga cccatcggct
     5701 agtgcgcggc cgcgtgcgga agcgctggta atgagcgctg tgaacggtgc cggctggggt
     5761 gacgctcatc gccggttctg ctcctgtttg cgaatcgccg tcatgaacat ctcctcgagc
     5821 gagctgcgct tggggtgcag cccgcgtacg gtagcgccgg cctggcgcgc cgcttcgaac
     5881 accagccgct cctgccccgc gcccggcaat tcgatcgcca gtacgccgtt cgattggttg
     5941 tgtacggcca cacgccgctg ctgaagctct ttgaccagat gctgcccatc accgctgtga
     6001 tccagcgtgt acagcgtcgg atgcgggcgg cgcatctcgg cgatactgcc ggatgcgagc
     6061 atctccccac cgccgagaat cacgacccgc tcgcacaccc gctccacgtc cggcagcagg
     6121 tggctggaga gcagcacgct gattccggcg cggccgaggt cgctgatcaa accgagcatc
     6181 gcttgccgtc cctgcgggtc catgccgttg gtcggctcgt ccagaaaaag cacgtcgggg
     6241 tcgtgaacga ttgcctgggc gagcttgagg cgctgcttca tgccggtcga atactgctcc
     6301 gccggccgat agcgggcctc gtccagccct acgtaatcca gcacctcgtg cgcccgctgc
     6361 atggcatcgc cgtgcgacag gccggccagg cgcgccgtgt agtagacgta tcccgtgccg
     6421 ctgctgccgc cgatcaggca gtcgttctcg ggcatgaagc cggcccgctg ccgcacgcgc
     6481 cggcgccgcc ggtgcacgtc cagcccgagc acttcggcgc gccccgaggt cgctggcgtc
     6541 tgacccaata ggatgctgat gagcgtgctc ttgcccgccc catttggacc caatagcccg
     6601 atcaggcccg cctccaactc caggctgagc cggttcaggg cagttacccg gccgtagcgc
     6661 ttcacgagtg attcacagcg gatcaacaca gggcgtagag ctttctcttc actggcgacc
     6721 agtcgtcatc gcatctggcc ggaattctac gaatgaacgc cctgccacgc cacatctgtc
     6781 tgtcagtttg tgtagcgcct cgtcctgcca caaaccaagt ccgccagtca tacgcgacgc
     6841 actgcgtgga acacaaacgg gcttgccctg ttgcaccgca attgccacct tggttcgcat
     6901 tcgggctgga attgcgcgag caacctcggc agttctatag ccggccgtgc cgaatgattg
     6961 caaccaacag catgaatccc agcactccgg cggccaggta gccgatcagc ccgatcagtg
     7021 gaatgtcgtg ccaggtcggc ggcagccgcg aatgcacgat caacgatgat cccactacca
     7081 gggccgccac gacgatcgca aacgagacgc ggttcgccac gcgctcgtgc gtgtccatca
     7141 gcttttccaa cccgcgatgc tcaaagttga tcttcagcgc cccgcgcttg gccagccgca
     7201 gcaggtcgcg cagcccactc ggcacctcgc gggccagatg ccacaattct tcggtcgact
     7261 cgtagacatc agccaggaag cgcttcggct tcaagcgttg caacttcaat cggcgaatat
     7321 acggctcggc tacggccatc atgttcagat cagaatcaag ccgtgccacc aagcgctcca
     7381 cagtcgcggc cgccttgagc atggtcacca ggtccggcgg cacactaagt tcgtattgtc
     7441 gaatcagccg catcagctcg tgtaccagcc gcccgaactg caggtcgccc agcgcgaccg
     7501 ggatgttcac gtcgatgaat tcggccacgt cgcgctccat ggcgcgcaag tccggctcaa
     7561 cgtcatccgg gggtttggcc agccgcagga gactgcggca gacccgcgcc gcatcgcggg
     7621 tggctacggt gtagatcaga tcagccacct gctcgcgcgt ctgccggtcg atccgcccca
     7681 tcatgccgaa gtcgagcagg cagatcacgt tgcccggcaa cacaaacgcg ttgccgggat
     7741 gagggtcggc gtggaagaag ccgtgtacga agatctgttt gagcgtcagt tcagctccgc
     7801 gggcggcaat gactttcgga tcaagacccg ccgcccgcag cgccgtggta tcggtcgcgg
     7861 gaatgacatc cacatattcg agcgtcagga cgcgccgcga cgacagctcg cggtagatct
     7921 tgggaatgta aatggttgga tcttgcgcga attgtcgggc gaagcgatca atatgcgctg
     7981 cctcgaccgt gaaatccaat tcccgctcca acacccggct gaattcctcg accatgcggc
     8041 tgggatggta gacgcgccat ccctcgacgt gccgttcgac cagcgtcgcc aggtgcagca
     8101 aaatttccag atcgaccgca atcatcgagc ggatgttcgg gcgctgcact tttacaacca
     8161 cctgttcgcc gctgaccagc gtggcccgat gcacttgcgc aatggacccg gccgcgatcg
     8221 gatactccgc gaactcggca tacagcccct caaggggccg gcccaactcg gtttcaatcg
     8281 tctcgcgcac ctgttcgaag gcgaacggcg gaacgtgctg ttgaagcttc gtgagttcct
     8341 cgatgaactc cggcggcagc acgtcggggc gggtcgagag cacctgcccc aacttgatga
     8401 aggtgggccc caattcctcc agcaccaggc ggacgcgctc ggccggagtc agcatttcga
     8461 cctgggccgg cttttcgcgc gacagcaaat gcaggccggt ttccaggtat tggctgatcc
     8521 gcagccggtc caccaacccc ccgaagccgt acttcacgag cacggtcagg atctggcggt
     8581 agcggttaat gtggcgatag gttcgcgaaa cgatcccgat gcgggggatg cgcatgatta
     8641 gtgcgacttg gccgccaggt cgcgctcgag cttttcaatt cgggcgacaa gctgggcgat
     8701 gtcctcgcgc gtggcgacgt tggtgcgcgc cagggcgtca ttgaccatgc gctgcacgtt
     8761 ggcctgcata tccgcccggg ccttctgcgc ccgcgctacc gcctcatcga cgaacgcctg
     8821 ccccttttcc gacgaaagct gcgcggagtt ggcgagttcg cgggccatct gctccacgcg
     8881 atccatcgtc atggctgccg caccgatccc cgccagaaag gttttcttca cgagatcgat
     8941 catgatgggt cctctgttgg gtcctgtccc agcttgattt tatcggattc gccgcggcac
     9001 agccagaata aaccgcttca gccgctttag cggccaatcg tggcccggct gttgtatcag
     9061 cggctattcg tcgtcacggc tgtagtagcc gtttctgggg cagcggcttt tagccggtct
     9121 gaccggttga gaaccggttc cacaggagca gccggacctt cggttcgagc catgcgccgg
     9181 aacagcacaa acgagctcaa agccagcccc agcgagatcg cgaatgagcc ccagtgcaga
     9241 tactggttcg ccagccaccc cagtatcggc atggttgccc cgcgcagacc ggtcagcccc
     9301 aggtgaattc ccatgtacag ctcgattttg tgctcgctgg cgaaatgcag gtggccgatg
     9361 ttccaggcca gatcgccccc gccgcgcgcc aggccgttta gaacgcgtgc cacgaccagc
     9421 acaccgatcc ccgcgatgta aaagaagcgg ccgtccaacg cgaacatggc catcgcgacg
     9481 atcaagccgg cataagagct ggcccaaacc agactgttgg aaactcggaa acgtagaacg
     9541 cccacccggt cgaagaactt cgaccagaag ctgatcgaaa ccagcacgac cagtatggga
     9601 agctgataaa tcagaaacgt cgagctccag tagccgaagc gctgcgccaa aatcgccacc
     9661 agcaccgggt cagtaaagaa attcgccgag cccagcagaa tcatggcccg catgtagtcg
     9721 gcgaagcgct ggtcggtgcg tagaatcgcc gccgcctctt tcagcccatg ccataggcgg
     9781 gccgaggacc cttccccgtt ctgctgcgcg gccgcgagcg ctcgaaagcg ccgcacttca
     9841 gccctctcac ggcgcacccg catgcgcctc agcggcagca gcgaaagcgc cccgagcgtc
     9901 acggtggcgg gatagaccag ccggtagtag tccgggttgc gatcgaacag gatgctgagg
     9961 agcgcggtcg tgaagactga gagcagcacg ttgagcatgt ggagccggcc ggctacctgg
    10021 gctcggcaga tccgcgagta attcgcgccc cacatcgtgg tccggatcgt caacatgccc
    10081 gataagaaga agtgcgtacc cgcgatttgg gccgcaaaga gccaagctga ccagggccgc
    10141 ccggccggcg tgaacgcgat gctgcaaacc aggaccagcg ccccggacgc cagccaagta
    10201 taagttttga tccgctgctg gccgcggagc agcaggcccc aggccaggtt gaagacattc
    10261 acgacaatcg gaatggcgaa tattaccgaa gtgagcaggc cgggcgccgc gaacgtcttg
    10321 gccgccacga cgctggcgat ggtgccttca accccgccgg tcacaaagcc ccacaaggcg
    10381 agatgccgca gctcggccat gtagttgcgg cgcgtcatca gcgacagccc gccggtttgg
    10441 agaaaatacg ctatgcgccg catcgaaccc tacgatagca ccgctaacac cttccgaaaa
    10501 cggccaaggc accggccagc acgttctgcg cccggccgaa tgagctgcgt caaaaatgcg
    10561 gacccaccgg ctgattcttt gacaaccgct ccggaccaag ggacaataat gtcggcggct
    10621 gacaagaatc aggtcgcgcg ccgaccgaac tagccgatcg gcccgcgagg tgcttggggt
    10681 ccgaagctcc ccggaacgcc gggcacttaa gaggaataat ggaatgattc tcgcctggct
    10741 tgatcactgg actattctct tggtcggact cgtggcgctg ctgcttttcg gcagccgctt
    10801 gcccgaagtc gcgcgctctc tgggccgctc gctgaacgaa ttcaagcgcg gtctaaacga
    10861 gatcaacgac gagatcgacc gcgccccgaa agacccaaac cagccaaagc ttcgctcgcc
    10921 gtccgagccc cggctggact cgcctgcgcc agagccgcgc gccgaggcta cgccggccca
    10981 aaataaggcg cagtatccgg aggcgccgcc ggaagggcct gcccagagct aatggcccca
    11041 agcgagcttg cccgcaagcc ggcttcaacg gcccgctact gatccgcggc cacaagcttc
    11101 aggacttcgc ccgtatcacc tgacggcccg gccactagcg acgtgagcac gtagacctcg
    11161 cccgccgagt cgcgcccgaa acctttcaca aaccgcccga gccgcccatt agtgccgttg
    11221 ctgattgcca cttcgctgaa cgaccacgtg ccgtcagccg cctccgtggc cgcgaacacc
    11281 ttgccgccca ctgagcccgc ggaaaagtcg ccgaggatgt agcgcccggc caggccgcca
    11341 atagccgaac cgcgatatac atatccgccg atgacggcgc tgccgaacgg gttgccggct
    11401 gcgtccgtgt gaggatattc gagaatcgga ttcagcagcg gttcgccgcg tgcgccggta
    11461 gtcgcgcagg tggcaggcgg attacctggg ctcgacgggt cgaagcataa tgtgccttcg
    11521 cggatgttcc acccataatt gccgccgctc tcgatcaaat cgatttcctc gcgtagggcc
    11581 tggccggcgt cgcccgcgaa caaccgcgtc tggccggact gcgtgtcgat cgagaagcgg
    11641 tacgggtttc gcaaaccata ggcccagatt tcggggcggg cggcatccac gttgaccagc
    11701 ggattgtccg gcggaatgcc gtacgggtcg ccgctgttga cgtcgattcg caatatcttg
    11761 cccaacaagc tggcaatgtc ctgggcattg cctaacccgg gcgtatgccc gaagccgacg
    11821 tcgccaccgc cgccgccgtc gccggtcgaa atatacaaga agccgtccgg cccgaacgcc
    11881 aggtcgccgc cgttgtgatt ggactgcggc tgcggaatgc gcagcagcac gcgctcgctg
    11941 gccgggtctg caacgttcgc gtccgctgag acgcgaaact ctgaaacgtg agtttcgtga
    12001 ttgaatcctg ccggaacatc ggcgcccctt ggggcgctgt aatagatgaa gaagcgcccg
    12061 ttgttcgcat agtctggatg aaatgccagc cccagcagtc cgcgctcgtc gccgccggca
    12121 ttcaactgca ccatgcggtc gcgcacgtcc aggaaaggcg tcgcgagcaa ggcgccgttc
    12181 gcgtccagga cgcggacaac tccaatctgg tcgacaatga agaagcgccc gctgccatca
    12241 ggcgcttgca tgaaacccac cggcgatacc agaccgcggg ccactgattg caccgccaca
    12301 ctggtgctcg tggccggcgc cggctgcgga cagcccggaa ccagaacgag cagaactgcc
    12361 aacccagcgt aacgaacgaa taagcgcgcg tttaccgacc cgttcaaaca ctgcgctcga
    12421 tgatccgcgc gcgggcaaca agcca
//
LOCUS       DAOSYS010000091        12424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4297_length_12424_cov_5.300833, whole genome shotgun sequence.
ACCESSION   DAOSYS010000091 DAOSYS010000000
VERSION     DAOSYS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12424
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4297_length_12424_cov_5.300833"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLU99_07325"
     CDS             <1..661
                     /locus_tag="PLU99_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO16904.1"
                     /translation="NENNPGDSETPPESEHAPRYLQLLALPGIIILIIVVIGLVLYLL
                     YGRDTRPSGTEEISAPPPVSAAPATPAAQKPGAPAFEAQAMAGQRVAFPSTYKGKLVM
                     VDFWATWCAPCISELPNIVETYNAFHERGFEVLGIALEKRTPPAEILRFVQKSNMPWP
                     QVYEGAWDIASKFGVRNIPAAFLVDGDTGQLLASGEALRGPRLRATIQKFLEQKKREP
                     Q"
     gene            658..1020
                     /locus_tag="PLU99_07330"
     CDS             658..1020
                     /locus_tag="PLU99_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOO16905.1"
                     /translation="MTENEPAATPQTFDFATLATALALFRIDCRRYPTTDEGLRALLQ
                     PPAEADVRQRWQGPYIGHAGQLQDPWGHDLQYICPGSHNPFSYDLSSAGPDGRHGSPD
                     DVCNWRKDAPSAAPPAAG"
     gene            1074..1304
                     /locus_tag="PLU99_07335"
     CDS             1074..1304
                     /locus_tag="PLU99_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16906.1"
                     /translation="MPAGLHNPFWITPVAAGSLLVIIGILIFSKPELLAYFVAGVFMA
                     AGIALIGFGVRMRGRVTYHRIDPNREDDVMFP"
     gene            complement(1368..1568)
                     /locus_tag="PLU99_07340"
     CDS             complement(1368..1568)
                     /locus_tag="PLU99_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16907.1"
                     /translation="MRKSLARRMKWAAAMLLCVIGLAGVTGCDKTLQATVQDGVIDVS
                     SSLLSAVMQAAMELALEQASGG"
     gene            1967..4408
                     /locus_tag="PLU99_07345"
     CDS             1967..4408
                     /locus_tag="PLU99_07345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOO16908.1"
                     /translation="MAAAEIRPALDGLQQAVREPLDRNNLGRLARMHTYCQILSETAQ
                     PADAQPNSALRQVAATLATLLERIILDEVAQPAAALALIPQAVRCLTDLFTEPSRPTD
                     QLLAQMAAALAGTLSDSGAPTAQATDSPPADACAAAPAVQVTPQAEAPQAGAAADCQP
                     PAASRAPVPAIAAYVSEPLVLDLEESEHLRGFIEESREHMEAIESGLLDVERDPADTA
                     KINELFRPFHTIKGIAGFLNLRDINQLTHEIETILDLGRKNELRITPHTVDRILAGVD
                     LLKEQIASIQNYLAEPRERVCPQPDISSIMFELRTIAAQRGQDAVAPDREPPKLGQLL
                     ISEAGVTREQIASALQKQALEPGGGRKVGEILVEQGAVTEEQVESALSRQMAAGQGEQ
                     SIRVDINKLDLLIDAVGELVIAQTMVNLHEGTRQNDALARHVSQVTKIVRDVQETAMS
                     MRMVPIGPTLQKMRRLVRDVSRKTGREVELHISGEETELDKTVIQRISDPLVHMVRNA
                     VDHGIEPPETRRQLGKPPTGNLWLDAYHQGDCIVIEVRDDGRGLDREKLITKAVQRGL
                     IGRDEQLTDQQAYALIMQPGFSTAEQVTDISGRGVGMDVVKRNVDALRGKIEIQSEPG
                     RGTTFLIRLPLTLAIIDGMIVRVGRERVVVPTVLIEQSLRPQQAHISSVQGRGEVMMV
                     RGELCPLVQLGELFGYSQRIDPCENIVIIVQCETRKIGLVVDELIGQQQVVIKSLAER
                     FKQVSGVSGAAILGDGRVGLILDPTGILQMHNRHVQSLRCAAPEPKFTPSETDAEYAV
                     DSTTAAEPLAAAVVN"
     gene            4430..4915
                     /locus_tag="PLU99_07350"
     CDS             4430..4915
                     /locus_tag="PLU99_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOO16909.1"
                     /translation="MQTPLTQSAATRGGKYLTFSLANEEYGLEILKVREIIGIMDITA
                     VPQMPEYVKGVINLRGKVIPVIDLRLKFGLPPAEYTDQTCIIVVDIGSLTGIIVDTVQ
                     EVLDIEAAQIDPPPPLGANVDTSFILGLGKVKDEVKILLDVDKVLSSEELSEIEQTLQ
                     G"
     gene            4968..5447
                     /locus_tag="PLU99_07355"
     CDS             4968..5447
                     /locus_tag="PLU99_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOO16910.1"
                     /translation="MALLVVDIADLAVSRDPETTLVTYSLGSCIGVSIWDPVVRAGGM
                     LHYMLPDSALSPEKAGNAPGMFCDTGVPRLFRAIYELGGIKSRLIVKVAGGSMLLDDR
                     GTFNIGKRNYLALRQLFWKNGVMIDGEDVGGSVSRTLRLHVGTGEVTVKTRDREVPL"
     gene            5444..6301
                     /locus_tag="PLU99_07360"
     CDS             5444..6301
                     /locus_tag="PLU99_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO16911.1"
                     /translation="MIARTLEQIVERVTEMPRLPDTAARLIQVINNPASSIEQIVEII
                     RYDPTVTADLLRLCNSAYFGLVRRVRSIDEATRLLGTAKVLQLVISGTMQVTLARPQR
                     GYGLAPGALWEHSVTVALACQSLAQRYAPPEAAVLFTAGLLHDLGKVVLSEYVAAEYG
                     EIMERVQGQNASFLDAEREVLGFTHAEIGARLAHNWSLPDTIVRCIRHHHDPDALGTP
                     DQLVDIVHLADAIGLLTGVGCGEVDGLAYAVQPAVLERCGANLGVLEQIGVDVVLELK
                     AVRGLFRMS"
     gene            6316..6738
                     /locus_tag="PLU99_07365"
     CDS             6316..6738
                     /locus_tag="PLU99_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO16912.1"
                     /translation="MAANVLIVDDSVTMRRMIKRTIEISGLDVAEVYEASNGIEALAR
                     MDQYSIGVVLLDINMPVMTGVQLIERLRDDERLREIPIIVASTEGSQTRITHLLEAGA
                     RGFIRKPFQPEQIRDALAPLVGLRAQPAQPAGLDESAF"
     gene            complement(6705..7325)
                     /locus_tag="PLU99_07370"
     CDS             complement(6705..7325)
                     /locus_tag="PLU99_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOO16913.1"
                     /translation="MTEETPYVSRAGLKLEAALRAFGIDVSGFVCCDFGSHTGGFVDC
                     LLQHGAARVYAVEPGYGVLDYNLRRDPRVVVCERTNALRYIPPERCDLVTIDVGWTQQ
                     RLILPAARRCLKPGGHVITLVKPQYEAPKEMLVTGLLPPARLAPVLGACRAEIEQLGW
                     SIRRQIESPLRGHGGNTEYVLWLSSKENATETRSPHQKADSSKPAG"
     gene            complement(7432..7860)
                     /locus_tag="PLU99_07375"
     CDS             complement(7432..7860)
                     /locus_tag="PLU99_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16914.1"
                     /translation="MAPLTSPEPATAPSAPATAVPEPQETPETPPAAAIEPAPAVPPE
                     APKPAEPELPPYIRVVERFRPSEPALVHLDDSSPERLVIETRNVRRLWIDRERAPLVT
                     NRSVVLRLDGQGIEWTGDSRTREFEKSVNGEWGPARERKR"
     gene            8184..10184
                     /locus_tag="PLU99_07380"
     CDS             8184..10184
                     /locus_tag="PLU99_07380"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16915.1"
                     /translation="MDCSNVRAASNEHDIPETLPSTGRQSGLHLPNLLVLNRNFVLLW
                     AAYGISAIGDHLSEMALLKERGGLERPDVTRLQALLTFGFFLPFVIFGPIGGWWADRF
                     SRKWSMIGADLARAGVMASLSFALPIAAGGTLISYLGARGWGDYSAALPLFFTGAFAA
                     FFAPARNALLPTLIRNDQIVRANALLSAMGTIGGILSAWLGGMLVDLSEAGHFDLHWN
                     YRLDGLTFLVSAACLLFIAMRKARQQLRPRVEGLVAPIVQGFRYVRQHRRVLQMILLG
                     TVFWAAAGVIISIVPALVRDVFGGRFSDAGLYRGLIAAGLAAGAAVLTLIGPAAPLQL
                     RVLTGLFWGAFWLFALEFAYVWRLGKFFTGLCLFGMGGAGAVILVTAMAMLQHLVPNS
                     RRGRVFGANEMATTASLVIATGLLGLPEIPNLDRYIPYLIAATGLMLLIALITAWREY
                     RRKDPFPAIMSFLWLIQRFYAQFWLRVERVGPCTVPRHGPVILAINHTAGVDPMAIYA
                     TCRHRLTGFVVAREYFEIPILGWFQRLANCIPIDRQNPTKSFLTSSLRFLRQGGCLAI
                     FPEGTFHDPHEPPPPVRPGVGVLALWSGATVIPCHISGTKYHSNPIRSYLARHKIRVR
                     YGAPVDLSAFAGRAKQREAQQEVSELIMAKIRELGPRDSACE"
     gene            10175..11731
                     /locus_tag="PLU99_07385"
     CDS             10175..11731
                     /locus_tag="PLU99_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16916.1"
                     /translation="MRVNKREIRDRSWLKPAHRRRGWMLLASAVLMPLVILLALRAFN
                     VPLGCPGRFVYLYSPPPVAGWRAAMVPFAAVIGAGLGLAIWLMSADDRARRVLGFVLL
                     AGGSLALGVWTYVAPPDHAVQHAFNMQSPSQDGAFLLEAFKFEHARDYLRAFPERAGT
                     PAELMRGTRIISNPPGMTMLAYLCRRLNEAAPPLANYLQRQFQVNDVDDPRPFLETAS
                     AGLLFTWVLTGVWLLAGVFLYRLGRLFFDPPVAGALCVCLLFSPTTLLFTPGKDTAQL
                     LTAAVALWLWFLAWHRKSAWLAAAAGAAFTLSSVVSLVHVWLAAIALAAVFVCRPWQW
                     RSLAGHLVAPALAGAVAAGALAYISCGLNIPATLVAVARSQAAVTRGADAMPWLWQSL
                     GVPLFLLLAGPAFWTACLSMRRIGAEVTPRGSDHRMGLGMLLSTLLVLIATVGFTNIE
                     TPRLWIPFSIPLLLGIALLSDAVRRPERRARVFLALLVAVQIAGSALIWALLDCREAE
                     HRLLEGRLFW"
     gene            11906..>12424
                     /locus_tag="PLU99_07390"
     CDS             11906..>12424
                     /locus_tag="PLU99_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16917.1"
                     /translation="MNVSKSAQELRRLFSGLVEQIFMAEVGICAPRLTDYLAQLLADF
                     LHMDRVFRLRTADGELIREVSRMRAEAEIGMRLGDQTRARHINRYIGDFTLFWAGLYP
                     ESLRPRRNLGADLWRQYLVEGKTGYELASELSQTEDVPPPQLLFDLSRQFESCVHGLH
                     LVRENWEQLPNLS"
BASE COUNT         2174 a         3975 c         3947 g         2328 t
ORIGIN      
        1 gaacgaaaat aatcccggcg actcggaaac gcccccggag agcgaacacg ccccgcgcta
       61 tctacaactc ctggcgttgc cgggaatcat catcctgatc atcgtggtga tcggactggt
      121 tctctatttg ctctacggcc gcgacactcg gccgtcgggg accgaggaga tttcggctcc
      181 gccgcccgtt tcggcggctc cggccacgcc ggcggcacag aagccgggcg cgccggcttt
      241 cgaggcgcag gccatggccg gccaacgggt tgcatttcca agcacttaca agggcaagct
      301 ggtgatggtt gatttctggg ccacctggtg cgcgccctgc ataagcgaat tacccaacat
      361 cgttgagacg tacaacgcct ttcacgagcg gggcttcgag gtgctgggca tcgcgctcga
      421 aaagagaacc ccaccggcgg aaatcctgcg attcgttcag aaaagcaaca tgccctggcc
      481 gcaagtttat gagggcgctt gggacatcgc gagcaaattc ggcgttcgca acatcccggc
      541 cgcctttctg gtcgatggcg acaccgggca attgctggcc agcggcgaag ctctccgtgg
      601 cccacgtctg cgcgctacca tccagaaatt ccttgagcag aagaaaaggg aaccacaatg
      661 acggaaaacg agcccgccgc cacgccgcag acgttcgatt tcgccaccct tgcaaccgca
      721 ctggcgttgt tccgcatcga ttgccgccgc tacccgacca ctgacgaggg gctccgcgct
      781 ttgctccagc cgccggcgga agccgatgtt cggcagcgtt ggcagggacc ctatatcgga
      841 catgcggggc aactgcagga tccgtgggga cacgatcttc aatacatttg tcccggcagc
      901 cacaatccgt tctcatacga cctttccagc gccggccccg acgggcggca tggcagcccg
      961 gacgatgtct gcaactggcg aaaggacgcc ccgtccgccg ccccgccggc tgctggctag
     1021 gtagctggcg cgcggctcaa gcgctcggcg cgttggaaag caagaggtgg agtgtgcctg
     1081 ctggcttgca taatccgttt tggatcacac ctgtcgcagc agggagtctg ctcgtcatta
     1141 ttggaatttt gatcttcagc aagcccgaat tgctggccta ctttgtcgcg ggggtgttca
     1201 tggcggctgg aatcgctctg atcggcttcg gcgtgcgcat gcgcgggcgg gtgacgtatc
     1261 accgcataga ccccaaccgt gaagacgatg tgatgttccc gtagcgcccc accgtcaggc
     1321 ccgcggctca ggtcctacca aacccgagcc gtcggcgatg caggttctca cccgccgctc
     1381 gcttgctcca gtgcaagctc catggccgcc tgcattacgg cactcagcaa cgatgaggaa
     1441 acgtcaatca ccccgtcctg aaccgttgcc tggagcgtct tgtcgcaccc ggtcacgccg
     1501 gccaggccga tcacgcacag caacatggcg gccgcccact tcattcgccg agctaagctc
     1561 ttgcgcattc acgcactcct caatagctgg tcaggcccat cctattcgcg acgcgcctgt
     1621 ttgaccagcg cttgttatcg gccgattccg accagcagaa cctgcacaat ttatattccg
     1681 ggcccctacg ccgggttgca gccctgctcc gtttgctcgc tgttagccat gcgactgcgg
     1741 ccgtgaaact gtggtgttca acgaacgcag cacgcgggtt gagccccggc tgatcttggt
     1801 tacgccgtcg ttatccggtt tatggggcgt gtcaattcaa tatggccttg tcacggcacg
     1861 atagcaccgc agagcgccgg aaaacttcgc gcgccgcacc ttccggacga cggccgcccg
     1921 ggtggcttgc gcaaacgtac aggaagctgg cgcaagagag acactcatgg ccgctgcaga
     1981 aataaggccc gctttggacg gcttgcagca ggcggttcgc gagccgctgg accgcaacaa
     2041 tttgggccgg ctggcgcgca tgcacaccta ctgccagatt ttgtctgaaa ctgcccagcc
     2101 cgccgacgcc caacccaaca gtgctctgcg ccaagtggcc gccacgctgg cgacgctgct
     2161 cgaacgcatc atcctcgatg aagtcgcgca gcccgctgcc gccctcgctc tgatacccca
     2221 agcggttcgg tgcctcacag acttgtttac cgagcccagc cgcccgactg accaactgct
     2281 ggcgcagatg gccgcggcgt tggcgggcac tttatccgat tcgggtgcgc cgacggcaca
     2341 ggctacggac tcgccgccgg cagacgcctg cgcggccgcg cccgctgtcc aagttacgcc
     2401 gcaggccgag gcgccgcagg ccggcgcagc ggctgattgc caaccgccgg ctgcttcgcg
     2461 ggcgccggtc ccggcaatcg cagcctacgt gagcgaaccg ctcgtcctcg atctggagga
     2521 aagcgaacac ttgcgcggct tcattgagga gagccgcgag cacatggagg ccattgagtc
     2581 ggggctgctc gacgtggaac gcgaccccgc cgataccgcc aaaatcaacg agctctttcg
     2641 gccttttcac accatcaagg gaatcgcggg ttttttgaac ctgcgcgaca tcaaccagct
     2701 cacgcacgaa atcgagacga tcctcgattt gggccgcaag aacgagttac ggatcacgcc
     2761 gcacacagtt gaccgcatat tggcgggcgt agacctgctc aaggaacaga tcgccagtat
     2821 tcaaaactat ctggcggagc cgcgggagcg ggtctgcccg caacccgaca tcagctcgat
     2881 catgttcgaa ttgcggacca tcgcggcgca gcggggccag gacgcagtcg cccccgaccg
     2941 cgaacccccc aagctcggcc aactgctcat ctccgaggcc ggcgtgacgc gcgagcaaat
     3001 cgccagcgcg cttcagaaac aagcgctcga gccgggcggc gggcgcaagg tgggcgagat
     3061 tctggtcgag cagggcgccg tgacggagga acaggtggaa agcgccctgt cacgccagat
     3121 ggccgccggc cagggcgaac agtcgatccg cgttgacatc aacaagctcg atctgctgat
     3181 cgacgcggtg ggcgaactgg tgatcgcgca gaccatggtc aacctgcacg aaggcacgcg
     3241 gcagaacgac gccctggccc ggcacgtctc gcaggtcacg aagatcgtgc gcgacgtcca
     3301 ggaaacggcc atgtccatgc gcatggtgcc catcggcccc acgctgcaga agatgcggcg
     3361 gctggtgcgg gacgtctcgc gcaaaaccgg ccgcgaggtc gagctgcaca tcagcggcga
     3421 ggaaaccgaa ctggacaaga cggtcatcca acgcatttcc gatccgctgg tacacatggt
     3481 gcgcaacgcg gttgaccacg gtatcgagcc gcccgaaacg cgccgccagc ttgggaagcc
     3541 gcccactggg aacctctggc tcgacgctta ccaccagggc gattgcatcg tcattgaggt
     3601 gcgcgacgac ggccgggggc tggaccgcga aaagctaatc accaaggcgg tgcagcgcgg
     3661 gctgatcggg cgcgacgagc aactgaccga tcagcaggcc tacgccctga tcatgcagcc
     3721 cggcttttcc acggccgagc aagtgaccga catctccggg cgcggcgtcg gcatggacgt
     3781 ggtgaaacgc aacgtcgacg cgttgcgcgg caagattgaa attcagagcg agcccggccg
     3841 cggcacgacc ttcctgatcc ggctgccgct cacgctggcc atcatagacg gcatgatcgt
     3901 gcgcgtgggc cgcgaacggg tggtggtgcc cacggtgctg atcgagcaat cgttgcggcc
     3961 gcagcaggcc cacatcagca gcgtccaggg ccgcggcgag gtgatgatgg tgcgcggcga
     4021 gttgtgccca ctggtacagc tcggcgaact gttcggttat tcgcagcgga tcgatccgtg
     4081 cgagaacatc gtcatcatcg tacagtgcga aacgcgtaag ataggattgg tagttgatga
     4141 gctgatcggc cagcagcaag tcgtaatcaa gtcgctggcc gagcgcttca agcaggtcag
     4201 tggcgtttcg ggcgcggcga ttctgggcga tgggcgggtg ggtctgattc tcgacccgac
     4261 cggcattctg cagatgcata accggcacgt ccagagcctg cgctgcgcag cgccggagcc
     4321 caagttcacc ccgagcgaaa cagacgctga gtatgcggtc gatagtacca cagcggcgga
     4381 acctctcgcc gccgcagtcg tgaattagga gaaccacgtg caagccgtca tgcaaacgcc
     4441 tctaacccag agcgccgcca cccgcggcgg caagtacctg accttcagcc tggccaacga
     4501 ggaatacggg ctggagattc tgaaagtgcg ggaaatcata ggcatcatgg acatcaccgc
     4561 cgtgccgcag atgccggagt acgtgaaggg cgtgatcaac ttgcgcggca aggtgattcc
     4621 ggtgatcgat ctgcgcctga aattcggcct gccgccggcc gagtacaccg atcagacctg
     4681 catcatagtg gtcgacatcg gttcgctgac gggcattatc gtggacacgg tccaggaggt
     4741 gctcgatatc gaggcggccc agatcgatcc gcctccgccg ctgggggcca acgtggacac
     4801 ttcgttcatc ctcggcttgg gcaaggtcaa ggacgaggtg aaaatactgc tcgacgtcga
     4861 caaggtcctc tcgagcgagg agctgagcga gatcgaacag actttgcagg gctgagccgc
     4921 cggagcaggc gcggcgggcg gcccaccgcg ccgcgcagaa gggacccatg gccctgctag
     4981 tggtcgatat cgcggatttg gcggtatccc gcgaccccga gaccacgctc gtcacctact
     5041 ctttgggtag ctgtatcggc gtgagcatct gggatccggt ggtgcgagcg ggcggcatgt
     5101 tgcattacat gctgcccgat tccgcactgt cgccggagaa agccggcaac gccccgggca
     5161 tgttctgcga cacgggcgtg ccgcgcttgt tccgcgccat atacgaactg ggcggcatca
     5221 agagccgcct gatcgtcaag gtcgccggcg gttcaatgct gctcgatgac cgcgggacct
     5281 tcaatatcgg caagcgcaat tacctggccc tgcgccagct attctggaaa aacggcgtaa
     5341 tgatcgatgg cgaggacgtg ggcggctcgg tcagtcgcac cctgcgcctg cacgtcggca
     5401 ccggcgaagt caccgtcaaa acgcgcgacc gggaggtgcc cttgtgattg ctcgcacgct
     5461 ggaacagatc gtcgaacgcg tcacagagat gccgcgcctg cccgacacgg ctgcgcggct
     5521 catccaggtc atcaacaacc cggccagctc catcgagcag attgtcgaaa tcatccgtta
     5581 cgacccgacg gtcaccgcgg acctgctgcg actgtgcaat tcggcctatt tcgggctggt
     5641 gcggcgcgtc cgctcgatcg acgaggccac ccggctgctc ggtacggcca aggtgctgca
     5701 gctcgtgatt tccggcacga tgcaggtcac cctggcgcgc ccgcagcgcg gctacggact
     5761 cgcgcccggc gcgctgtggg agcattcggt gaccgtcgcg ctggcctgcc aatcgctcgc
     5821 gcagcgctac gcgccgccgg aggccgccgt gcttttcacg gctggtctgc tgcacgactt
     5881 gggcaaagtg gtgctgagcg aatacgtcgc ggccgagtac ggcgagatca tggagcgcgt
     5941 gcaggggcag aatgcctcgt ttttagatgc ggagcgggag gtattgggct tcacgcatgc
     6001 ggagatcggg gcacgactgg cgcacaactg gagcctgccg gacacgatcg tgcgctgcat
     6061 tcgccatcat cacgacccgg acgcgctggg cacgccggac cagttggtgg acattgtgca
     6121 cttggcggac gccatcgggc tgctgaccgg cgtcggttgc ggcgaagtcg acggcctggc
     6181 gtatgcggtc cagccggccg tgctcgagcg gtgcggcgcg aatcttggag tgctggagca
     6241 gatcggcgtg gacgtggtgc tggagctgaa agcggtgcga ggtttgttca ggatgagcta
     6301 gccaggaaga ctactatggc ggcgaatgtg ttaatcgtag acgattcggt gaccatgcgg
     6361 cgcatgatca agcgcaccat cgagatcagc ggtttggacg tggccgaagt gtacgaagcg
     6421 tccaacggca tcgaggccct tgcgcgcatg gatcaatatt cgatcggggt cgtgcttctg
     6481 gacatcaata tgccggtgat gaccggagtg cagttgattg agcgcttgcg cgacgacgag
     6541 cggctgcgcg aaattccaat catcgtggcc tcgaccgaag gcagccaaac gcgcatcacg
     6601 cacttgctcg aggccggggc gcggggcttc atccgcaagc cgttccaacc tgaacaaatc
     6661 cgcgacgccc tggcgccgct ggtggggctg cgggcgcagc cggctcagcc ggccggcttg
     6721 gatgaatcgg ccttttgatg gggggaacga gtttcagtcg cattttcttt tgaagacagc
     6781 cacaacacgt actcggtatt gcccccatgc ccgcggaggg ggctctcaat ttggcggcgg
     6841 atcgaccagc ccagttgctc gatctctgcg cgacaagcgc ccaggactgg tgcgagtcgc
     6901 gccggtggca acaggcccgt tacaagcatc tctttgggag cttcgtactg cggcttcacg
     6961 agcgtgatta catgtccacc gggtttgaga cagcggcggg cggccggcag gatcagccgc
     7021 tgctgcgtcc aaccgacgtc gattgtcacc aggtcgcacc gttccggtgg aatgtacctc
     7081 agtgcgttag ttcgttcgca aacgaccacg cgcgggtcgc gccgcaggtt gtagtcgagc
     7141 acgccgtatc ccggctcaac cgcgtacacg cgggctgcgc cgtgctgcaa taggcagtcg
     7201 acgaacccgc cggtatggct gccaaagtcg cagcatacga acccgctgac gtcgattccg
     7261 aaggcgcgca gagcggcttc cagcttcaac cccgcgcgag agacataagg cgtctcctcg
     7321 gtcatacgtg tcaacttcag ttatcagtat gccagttctc agtctcgcgg gtttaacgct
     7381 tgttgcccag cgactgacaa ccggtaactg caaatagaca actgattcct gctaccgctt
     7441 ccgctcgcgg gccgggcccc actcgccgtt taccgatttc tcaaattcac gcgtgcgcga
     7501 atcgcccgtc cactcgatgc cttgcccgtc caggcgcagt acgacgctac ggttcgtcac
     7561 cagtggggca cgttcgcgat cgatccatag ccggcgcacg ttacgcgtct cgataaccag
     7621 ccgctcgggt gaactgtcgt ccaggtgaac caaagccggc tcggaaggcc ggaagcgctc
     7681 cactactcga atgtatgggg gcaactccgg ctccgccggc ttaggcgctt caggcggcac
     7741 tgcgggggcc ggctcgatag ctgcggccgg gggtgtctcg ggcgtttcct ggggctccgg
     7801 gacagccgtc gccggagcgc tgggcgccgt cgccggctcg gggctcgtaa gaggcgccac
     7861 gggcgtttcg tcgggcgcgg cggccggaga cgtgcgccgc gccgacccct cgcaaccggc
     7921 caacagcgct aaaaaagctg gaagagcgat ctttgccgca cggcagaatc ggttctgcgt
     7981 cattggctgc cctcttaagt taccaaaacg accagaccgc cgaggtctca acatcttata
     8041 cagatcaggc ggtttcgggc gcgtgaaacg gcaatttctc caccgcccag gcgccgaagg
     8101 cgcgtgccgc cgctttacca gcggatggcc cgtccgtaaa gtctttccgc cacggacacc
     8161 cggctgtcgt ccggcgtaca ctggtggact gctcgaacgt gcgtgctgcg tcaaacgaac
     8221 acgacattcc tgaaacgctt ccgagcaccg gccggcaaag cgggctgcac ctgcccaatc
     8281 tgcttgtcct taaccgcaat ttcgttctgt tgtgggccgc ctacggcatc agcgccatcg
     8341 gcgaccacct gagcgagatg gcgctcctca aggagcgcgg cgggctggag cggccggacg
     8401 tgacgcgcct gcaggccctg ctgacgttcg gtttctttct cccgttcgtc attttcgggc
     8461 cgatcggcgg gtggtgggcc gatcgattca gccgcaagtg gagcatgatc ggcgccgacc
     8521 tggcccgggc cggcgtcatg gcgagcctgt ccttcgcctt gcccatagcg gcgggcggaa
     8581 cgctgatctc ttacttgggc gccagaggct ggggcgacta ctcggcggcg ctgccgttgt
     8641 ttttcacagg cgccttcgcg gcgtttttcg ccccggcccg caacgcgctg ctgccgacgc
     8701 tgattcgaaa cgaccagatc gtccgcgcaa acgcgctgct gagcgccatg ggcactatcg
     8761 gcggcatcct cagcgcctgg ctgggtggca tgctggtcga cttgagtgaa gccgggcatt
     8821 ttgatctgca ttggaattac cgcctggacg gcctgacctt cctggtcagc gcggcctgcc
     8881 tgcttttcat cgccatgcgg aaagcaaggc agcagcttcg gccgcgagtt gagggcttgg
     8941 tagcgccgat cgtccaaggc ttccgttacg tccgccagca ccgtcgcgtc ttgcagatga
     9001 ttctgctggg caccgtgttc tgggccgcgg cgggggtaat cataagcatc gtgccggccc
     9061 tggtgcgcga cgtgttcggc gggcgcttct cggacgccgg gctctatcgc ggattgattg
     9121 cggctggcct ggcggctggg gcggccgtgc taacgctcat tggtccggcg gcgccgctgc
     9181 aattgcgcgt gctgaccgga ttattctggg gtgcattctg gctttttgca cttgagtttg
     9241 cctatgtctg gcggctcggc aaattcttta ccggcctctg cctgttcggg atgggcgggg
     9301 cgggagctgt gattctggta accgcgatgg ccatgctgca acacctcgtc ccgaactcgc
     9361 gccgcgggcg cgtgttcggc gcgaatgaaa tggccactac cgcctcactg gtgatcgcca
     9421 ccgggctgct gggtctgccg gaaattccga acctcgaccg ctacatacct tatctgatcg
     9481 cggcaaccgg cctgatgttg ctgattgccc tgatcaccgc gtggcgcgag taccgtcgga
     9541 aggacccctt ccctgccatc atgtcgttcc tgtggctgat acagcgcttc tatgcccaat
     9601 tctggctcag agtggagcgc gtcggaccct gcaccgtgcc gcggcacggg ccggtgatac
     9661 tggcgatcaa tcacacggcc ggcgtggatc cgatggccat ttacgccacc tgtcgccatc
     9721 gcctgaccgg cttcgtagtg gcccgggaat acttcgagat tccgattctg ggctggttcc
     9781 agcgcctggc caactgcatt ccgatcgacc gtcagaaccc caccaagtcc tttctgacct
     9841 ctagccttcg cttcctcagg caaggcggct gcctggccat tttccctgaa ggcaccttcc
     9901 acgacccgca cgagcccccg cccccggtgc ggccgggcgt gggagtgctg gcgctgtgga
     9961 gcggggccac cgtcatcccg tgccacatca gcgggacgaa ataccactcc aatccgatac
    10021 ggtcctatct ggcgcgccac aaaatccggg tgcgctacgg cgcgccggtc gatctgtccg
    10081 cattcgccgg gcgcgctaag cagcgcgaag cgcaacagga ggttagcgag ctgataatgg
    10141 cgaagattcg cgagcttggt ccaagagact ccgcgtgcga gtgaacaagc gcgagattcg
    10201 ggatcgtagt tggttgaagc cggcgcaccg gcggcgcggc tggatgcttc tcgcgtcggc
    10261 cgtcctcatg ccgctggtca ttcttctcgc gctgcgcgcg ttcaacgtgc cgctgggctg
    10321 ccccgggcga ttcgtctacc tctactcgcc gcctccggtc gccggctggc gtgccgcaat
    10381 ggtccccttc gccgcggtca tcggcgccgg cctaggcttg gcgatatggc tgatgagcgc
    10441 cgacgaccgg gcgcggcgtg tactcggctt cgtgcttctg gcgggcggct cgttggcgct
    10501 tggcgtctgg acgtatgtcg cgccgccgga ccacgccgtg cagcatgcct tcaacatgca
    10561 gtcgccctcc caggatggcg ccttcctgct tgaggcattc aagtttgagc acgcgcgaga
    10621 ctacttacgc gccttccctg agcgggcggg cacgcctgcc gaactcatgc gcggcacgcg
    10681 tatcatcagc aacccgccgg gcatgacgat gctggcgtac ctctgccgcc gcctgaacga
    10741 agcggctccg cccctggcaa actatctgca acgccagttt caagttaacg acgtcgacga
    10801 cccgcgccct ttcctggaaa cggcctcggc cggcctgctg ttcacctggg tcttaaccgg
    10861 cgtgtggctg ctcgcgggcg tgtttttgta ccgcctgggt cgcctgttct ttgatcctcc
    10921 ggtcgcgggc gcgctgtgcg tttgcctgtt gttcagccca acgacgctgc tgtttacgcc
    10981 gggcaaggac actgctcaac ttctcacggc tgccgttgcg ctctggctgt ggttcctggc
    11041 gtggcaccgg aaatcagcgt ggctggcagc cgccgcggga gcggccttca ccctttcctc
    11101 tgtggtcagc ctggtccacg tgtggctggc ggccatcgcg ctggccgcgg ttttcgtatg
    11161 ccggccttgg cagtggcgct cgctcgcggg acacctagta gctccagctc tggcgggcgc
    11221 cgtggcggcc ggcgcgcttg cttacatttc gtgcgggctg aacatcccgg ctacgcttgt
    11281 tgcggttgcg cggtcgcagg ccgccgtgac gcgcggcgcc gacgccatgc cgtggctgtg
    11341 gcagagcttg ggcgtgccgc tgtttctgct attggcggga ccggcctttt ggaccgcctg
    11401 cctgtcaatg aggcggatcg gcgcggaagt gacgccccgg ggatctgacc accggatggg
    11461 tctgggcatg ttgctgagca cgctgctcgt gctgatcgcc accgtcggct tcaccaacat
    11521 tgagacgccg cggctgtgga ttcctttctc cataccgttg ttgctgggta tcgcgctgct
    11581 atcggacgct gtgcggcgtc ccgaacggcg ggcacgcgtc tttctcgcgt tactcgtggc
    11641 ggtgcagatt gccggctccg cactcatctg ggccctgctg gattgccgtg aagccgagca
    11701 tcgcctgctt gagggccgcc tgttctggta aaaagcgtct ttacggggtg gagactgccg
    11761 aagccgggac ttggggtcat tgagcggctt cagaatctta cgcgcccgac atgattttgc
    11821 acgtgacttt gcgcgggaaa atacgaacaa ttctattaga gccaacgaag tcgccgactc
    11881 caagtatgca gaggacctag cggcgatgaa cgtcagcaaa tccgcccagg aactcaggcg
    11941 tttgttcagc ggcctggtcg agcagatttt tatggccgaa gtcggaatct gcgctccccg
    12001 tcttacggac tacctggcgc agctcctcgc cgatttcctg cacatggatc gcgtttttcg
    12061 ccttagaacc gcggacggag agcttatccg cgaggtttcc cgcatgcgcg ccgaagccga
    12121 aatcggcatg cgcctcggcg atcagacccg ggcacgccat atcaatcgct acatcggcga
    12181 tttcacgctg ttctgggccg gtctctaccc cgaaagtctc cggccgcgac gcaatctcgg
    12241 cgccgacctg tggcgccagt acctggtcga gggcaagacc ggctacgaac tggccagcga
    12301 gctgtcacag acggaagacg tcccgccccc ccaattgctc ttcgatctga gccggcagtt
    12361 cgaatcctgc gtccacggcc tgcacctggt ccgcgagaat tgggagcaac tgccaaacct
    12421 gtca
//
LOCUS       DAOSYS010000092        12423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4298_length_12423_cov_5.485446, whole genome shotgun sequence.
ACCESSION   DAOSYS010000092 DAOSYS010000000
VERSION     DAOSYS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12423
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_12423_cov_5.485446"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLU99_07395"
     CDS             <1..614
                     /locus_tag="PLU99_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16918.1"
                     /translation="GAWVVRPYSGRLETLGWAYIWVAVVVIMALGVDIDRNQFIFWLV
                     VVALIWVFGLWLKDVKQITVVGDIVRWFGQRDVQYNRALGLTISVILLVPFVLLMCWA
                     VINDRWRITHNEFEHYSFGRTDESLGRGAKTIRTTFPDVLEFLLGLAGTLIVYNASGT
                     RELRRIPHVMFLPLVRRRLNQVLESTQVTPSGAADEEDDDEQL"
     gene            852..2018
                     /locus_tag="PLU99_07400"
     CDS             852..2018
                     /locus_tag="PLU99_07400"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16919.1"
                     /translation="MLTHVKIRNFGCCADTDVLLGTPVVGLVGRNGAGKTTILQAIEW
                     LALSGTASESKRPGASLDAPRRVAASFDICGHQYSFSAVGRYLKSKGTGFDVVQGEER
                     LTKESEDSPKLVFYRRGEEVHTPLRDEPILIGAEAPSLGALASLLPSPDPLLQVIAPV
                     RDYLGRIRYYTLAEPEGQSDIIKLSDYQEWARSFEAGDSAADFVAYRILYMHEQDNES
                     FRELCDLVGPSGLGLIDGIDIVFYPQGDVEKRQFCMPWFRPGANLGGHGMSVPYSALS
                     AGTKRILRLLVHLLFDRGSVMLLEQPEDCVHPGLLYKLLDILRAYSDQCQVIFSTHSL
                     EVLSTLRPEEVRLVEARNGSTTCRGLSRQELQAAAQFLASDGSLGEFVESLDAG"
     gene            2750..2965
                     /locus_tag="PLU99_07405"
     CDS             2750..2965
                     /locus_tag="PLU99_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16920.1"
                     /translation="MGTNQVLLWSVLSAFAIAAGWWLAARPRASMRGIAVPVTALMLV
                     LSVLVLLLSSTGQDFRGFLLSVRELTS"
     gene            complement(3032..3973)
                     /locus_tag="PLU99_07410"
     CDS             complement(3032..3973)
                     /locus_tag="PLU99_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16921.1"
                     /translation="MFCMILAMDAKCDYRDVAGASLLDGLWFAAAISAADQGDAQLQG
                     QIDDLSGQVEDLSGRTQPGPAGLACWDLNGNGQPDAEEDINADGVWDARDCQGIAGQD
                     GSNGQNGTNGANGANCWDTIGDYNADGQCDSQDCLDWVVAHIDVSPGPAGDNCWDTIG
                     DYNEDGVANGDDCLDWVADHTQVDGTGDSSIIARGYVDSFGYLHGGQNILSTNLIQTE
                     RFDVAYEVVVDLTGLDADLQELTPNDFPVLITVYAIAADPWAWSWNIAALVAHYQYDG
                     ETSLDREAGTLTLRVHILDAQSGEHVGANFSIAVLKP"
     assembly_gap    4292..4391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4511..4597)
                     /locus_tag="PLU99_07415"
     tRNA            complement(4511..4597)
                     /locus_tag="PLU99_07415"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4561..4563),aa:Ser,seq:cga)
     gene            5028..6047
                     /gene="gap"
                     /locus_tag="PLU99_07420"
     CDS             5028..6047
                     /gene="gap"
                     /locus_tag="PLU99_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOO16922.1"
                     /translation="MAIRVAINGFGRIGRLVMRAGIKDPQLEFVAVNDLFPPASLAYL
                     FKYDSTHGKWPGQVEATEAALRIDGKEIRVCGEKEPAKLPWKDLKIDYVIESTGLFTA
                     REGASQHLKAGAKRVVISAPAKDKDIPTFVLGVNEEKYNPQTDTILSNASCTTNCLAP
                     MAKLINDNFGLEEGLMTTIHAVTATQPTVDGPSKKDLRGGRGAGQNIIPASTGAAKAV
                     TLCIPELKGKLTGMAFRVPTPDVSCVDLTCRTAKETSMSQINSVFKAAAEGKMKGILG
                     YTEEPVVSSDFISSAFSCVYDATAGMELNPRFFKLVGWYDNEYGYSNRVVDLLKYIGK
                     RDGLI"
     gene            6162..7412
                     /locus_tag="PLU99_07425"
     CDS             6162..7412
                     /locus_tag="PLU99_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOO16923.1"
                     /translation="MNKKSVRDLNVSGRRVLIRADLNVPLDKNRNVTDDRRIRMFLPT
                     LQHVLKGGGRAIVISHLGRPSGNPAEDQAFSLRPVAAKMEQLLGRPVKFASDCIGPEA
                     DAAVQGLKDGEVLLLENLRFHKAETVIDKKKKNPDGKLTPEQEAARQKFAQALAPHGE
                     IYVNDAFGTCHRQHVSMYDVPLLTPKGNRVVGFLVEKELQYLGQALQSPKRPFVAILG
                     GAKVSDKIGVIKNLLQRVDQILIGGAMTYTFWAAQGKSVGKSLCERDKLDLAKSLLEQ
                     GGGKIVLPVDSVAAAELKAGVATQVIAGDLPADMMGLDIGPKTIELFRKYIQQAGTIV
                     WNGPVGAFETPPFEKGTFELARAIAEATDRGAVTVIGGGDSAAAVEQAGLAERMSHIS
                     TGGGASLEFLEGKPFKTIEVLDDA"
     gene            7435..8118
                     /gene="rpe"
                     /locus_tag="PLU99_07430"
     CDS             7435..8118
                     /gene="rpe"
                     /locus_tag="PLU99_07430"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOO16924.1"
                     /translation="MTTIRIAPSVLSADFGYLAEQIAVVERAGADLLHLDIMDGHFVP
                     NLSLGVPVVASIRRHTGMFLDAHLMITDPGRYAPAFVKAGADSITFHIEVAERPRELI
                     KQIRDLGVKVGVSLNPGTPAERIFDILDLVDLVLVMTVWPGFGGQEFMRECLPKIEAI
                     AARLNANQWLQVDGGVDNKTAPLVVAAGADTLVAGSAIFGHADPGSAVIELRRAAQAS
                     ARSGTETRL"
     gene            8115..8696
                     /locus_tag="PLU99_07435"
     CDS             8115..8696
                     /locus_tag="PLU99_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOO16925.1"
                     /translation="MKVVLIPCAATEWREEGRLLGRVELSPCTDARQECEEWAAELQA
                     AGLAQIYHGPDELSHETARWLARWLTVPAKRADQLAEVDLGLWAGLTDQQLKSRYASA
                     HRELREAPLNVNPPEGENLGDAEERLRAFVKKQLKRNGKAAVGLVLRPLSFAMLWRSL
                     AGQQAPEVWEGMRHWPRPVVIDCSSEQPVFSGD"
     gene            8789..9613
                     /gene="accD"
                     /locus_tag="PLU99_07440"
     CDS             8789..9613
                     /gene="accD"
                     /locus_tag="PLU99_07440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOO16926.1"
                     /translation="MGESPIPVRRNVDVPEGLWIRCVKCGAMVYRRILEEELRVCPEC
                     DYHYRIDARTRLQQLNDPGTFEEFLPDLESTDPLQFTDRIGYKDRLEEAKAASNEREA
                     IVVGKGFIKGRPVIMGVMNPDFIMGSMGAVVGEKVAAAAERAIEEELPLIMVTCSGGA
                     RMMEGMVSLVQMAKTSAAIAKLDAAGGLYVVVMTDPTTAGVAASFAFLGDVTLAEPGA
                     QIGFAGPRVIKNTIKAELPEGFQTAEFMLEHGFVDRIVHRKDLRSEIARVIDYCNK"
     gene            9707..10066
                     /locus_tag="PLU99_07445"
     CDS             9707..10066
                     /locus_tag="PLU99_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HOO16927.1"
                     /translation="MLPSWFLEKLLDPLGLFGLAAQSCFMLRFVVQWFVSERRGRSYV
                     PVAFWYLSLVGGAMTFIYAVLRQDPVFALAQALGLAIYTRNLVLIYRRRSRTAARHPR
                     SDAEVARAGAGAMPNAD"
     gene            10202..10483
                     /locus_tag="PLU99_07450"
     CDS             10202..10483
                     /locus_tag="PLU99_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16928.1"
                     /translation="MRLDWVWADENPFAVAAEHALYHVANSFVTGLQHGEATWLERGL
                     THRLLGGHKHMLAATGERCEAITLDSDGSESWQAGCIEICPGLPLVRLG"
     gene            complement(11607..>12423)
                     /locus_tag="PLU99_07455"
     CDS             complement(11607..>12423)
                     /locus_tag="PLU99_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOO16929.1"
                     /translation="QMGPVLDGVAAHMSEYPEDQAFRVADAPPCDVARVLRESRTDVL
                     ICYMPVGSEDAARHYARACLDAGAAMVNCIPVFIASHPEWAEEFRRRNIPVVGDDIKS
                     QFGATILHRALARLMCDRGVKLARTYQLNTGGNTDFLNMLSQSRLKSKRISKTESVQS
                     QLDTPLEPINIHVGPSDYVPWQKDNKVCFLRMEYLGFGDVPMNLELRLSVEDSPNSGG
                     MAVDAIRCAKLALDRGIGGPLVEISAVTMKHPPQQFRDSIARENLERWIAGNT"
BASE COUNT         2319 a         3461 c         4110 g         2433 t
ORIGIN      
        1 agggggcctg ggtcgtcagg ccgtattcgg gtcggctcga aacgctgggg tgggcgtaca
       61 tctgggtggc ggtcgtggtg atcatggctt tgggcgtgga catcgaccga aaccaattca
      121 tattctggct ggtggtggtg gccctgatct gggtcttcgg gctatggctc aaggacgtca
      181 agcagatcac cgttgtcggc gacattgtgc gctggttcgg gcagcgggac gtgcaataca
      241 accgcgctct gggattgacg atcagcgtga ttctgctcgt cccgttcgtg ctgctgatgt
      301 gctgggccgt gatcaatgat cgctggcgca tcacacacaa cgaattcgag cactactcgt
      361 tcggccgcac ggatgaatcg ctgggacgtg gggccaagac cattcgcacg accttcccgg
      421 acgtcctgga atttctgctg gggctggcgg gaacgctgat cgtgtacaac gccagcggca
      481 cgcgcgaact gcgtcgcatc ccgcacgtga tgttcctgcc gctggtccgg cggcggttga
      541 accaggtttt ggaaagcacg caggttacgc cgtcgggggc cgcagatgag gaagacgacg
      601 acgagcagct atagtatgac tggtatgcgc aagcgtggcc ccgcccgacg aatcggcgag
      661 atgctgctcc ggcgcccctc cgcgaggcac gagagcggaa cgtcgcgagt tttcgggtgt
      721 gccgcgcgtt gggttattgc gcacctgaag ggccgttgag gcggtgtttg tccgcctccg
      781 atccgcccga taaagtcgct aggtagcggg cctgggtccg cggtaatgcc gaaagcagaa
      841 cggtgcttgt catgctcacg cacgtgaaaa tccgcaactt cggttgttgt gccgacactg
      901 acgtgctgct gggtacgcct gtagttggcc tagtgggccg gaatggcgcc ggaaagacca
      961 cgatactgca ggcgatcgag tggctcgcgc tgtcagggac ggcatctgag agcaaacgcc
     1021 cgggtgcatc gctggacgcg cccaggcgtg tcgctgcgtc gtttgacatt tgcgggcacc
     1081 aatattcgtt cagcgcggtt gggcggtatt taaagtcaaa ggggaccggt tttgatgtag
     1141 ttcagggcga agagcgtctg acgaaagaat ccgaagactc accaaagttg gtgttctacc
     1201 gtaggggcga ggaagtccac actccgctca gagacgagcc cattctcatc ggtgcggaag
     1261 ccccgtcgct tggggcgctt gcctcgctcc ttccgtcccc agatcctctt cttcaggtca
     1321 tagcaccggt ccgcgattac ctcggcagga taaggtacta cacgctggcc gagccagagg
     1381 ggcaatcgga cataattaag ctttcagact accaagaatg ggcccgttca ttcgaggccg
     1441 gcgattcggc ggcggatttc gtcgcatacc gaatactgta catgcacgag caggacaacg
     1501 agtcgtttag agagctgtgc gatttggtgg gcccgagtgg cctgggcctc atcgacggga
     1561 tagatatcgt attttacccg caaggggacg tcgaaaagag gcagttttgc atgccatggt
     1621 ttcgaccagg agccaatctt ggagggcatg gcatgtctgt tccctattcc gcgctttccg
     1681 caggcacgaa gcgcatactc aggctcttgg ttcatctgct gtttgaccgt gggagtgtca
     1741 tgctgttgga gcagcccgag gattgtgtcc atccgggatt gctgtacaag ctgctcgaca
     1801 tcctgcgagc ctactcggat cagtgccaag tcatcttctc gacacattct ctggaggtgc
     1861 tgagtacact aaggcctgag gaggtcaggc tggttgaggc tcggaacggc tcgacgacct
     1921 gccgtggtct gagtcggcaa gagctccagg cagccgcgca attcctcgcc agtgacggat
     1981 ccctcgggga gttcgtcgaa tcgttggacg caggctgaac atgttcgcta ttcttggtga
     2041 gaacaggtcc gacgcggcca cattgaaagt cataactcgc cgccttttaa aacacgactc
     2101 tattagcatc aagcagaagg gctaccacgg gtgcggggaa ctgtgtacta aaggcgccag
     2161 gcagatacgg ctcttcgccg atcagggcgt taagcgtttc atcgtctgta tggacgctga
     2221 cggagcggat ccgaaaccag ctcatgatcg ggttctcgag tgcgtcattc gtccaagcgg
     2281 cgttagcgat gcgagttgta tcgttgtacc ggtgcaagag ctggaagcgt ggattctggc
     2341 cgacaatgaa gctgtggcat gcgtgatccc gtcattcaag tttagtgcat gcagcaaccc
     2401 agagtcggtc gccagcccaa aggagtacct tgagcgactt tcgcggcagg gtcgttccag
     2461 acctctttat agccatgcca cgcacaacga acgggttgcc gagcaccttg acctcgagag
     2521 gttatggcgc gtttgtcctt catttcgctg cttgtacgaa tttgttagcg gcgcggcttc
     2581 agaatagctc ctgttgtctg gcggtagaga ttctcgggcc tttcgggacg cgcggcggca
     2641 ggggcttgtg aaagcaaatt tcgcgcccgg ggcaatctat tcgtatattt gaattgtgtg
     2701 atcagactct cggacggtcc ggccgtgcaa gttcaggagc gggaagaaca tgggaacgaa
     2761 tcaagtgctc ttatggtcgg tgttgtcggc gtttgcgatt gccgccgggt ggtggctggc
     2821 agcccgtcca cgggcgagta tgcggggcat cgccgtgccg gttacggcgt taatgctggt
     2881 cctgtcggtg ctggtgctgc tgctctcgtc caccgggcag gatttccggg ggttcttgct
     2941 gagcgtgcgt gagctcacct cttgatataa aaaccgggcg gtccggaaaa aaaccggacc
     3001 gcccgtggaa gcacctcgcc agtgcgaagg atcagggttt gagcacggcg atgctgaaat
     3061 tggcgcccac gtgctctccg ctctgagcat cgaggatgtg gacgcgcaag gtcagggtgc
     3121 cggcctcacg gtctagagac gtttcgccgt catactggta gtgggccacg agcgccgcaa
     3181 tgttccagct ccacgcccac ggatcggcgg cgatggcgta aacggtgatg agtacgggga
     3241 agtcgttggg agtcagctcc tgcaagtctg catccaggcc ggtcaggtcg accactacct
     3301 cataggccac gtcgaatcgc tcggtctgaa tcaggttggt gctcaggata ttttggcctc
     3361 catgcaggta gccgaagctg tcgacgtagc cacgcgcgat gatcgaactg tcgccagttc
     3421 catcgacctg cgtgtggtcc gcaacccaat ccaggcagtc gtcgccgttt gccacgccgt
     3481 cttcgttgta gtcgccgatc gtgtcccagc agttatcgcc ggcgggtccg ggcgaaacgt
     3541 cgatgtgcgc gactacccaa tccaggcagt cctgcgagtc acactgcccg tcggcgttgt
     3601 agtcgccgat ggtgtcccag cagttggcgc cattggcccc gtttgtgccg ttctggccgt
     3661 ttgagccatc ctgcccggcg atgccctggc aatcccgggc gtcccatacg ccatcggcgt
     3721 tgatgtcctc ttcggcatcc ggctggccgt tgccgttcag gtcccagcac gccaaaccag
     3781 cggggccggg ctgtgtacgg ccggacaaat cctcgacctg gccggacaag tcatctattt
     3841 ggccctgcaa ttgggcgtcg ccctggtcag cggcggatat cgcggccgcg aaccaaagcc
     3901 cgtccagcaa gctggcaccg gcgacgtccc gatagtcgca cttggcatcc atggccagga
     3961 tcatgcaaaa cattccgaat cccaagatag gcgcggaaaa cagcctcata tgaatctcct
     4021 ccgagacaag gtgccttaaa gcgtctctct agagtgaccg gcgaagtcat gcccgcaaat
     4081 ccgcaactaa ggcaggcact caatactagg cagccaactc gaaaaaaatc ctggaaagct
     4141 cacacaattt gtctacaaat tgccggcctg gaaccagggt caaatcgggc cggccgcggt
     4201 tgggccggta aaggtggccc gcagccatct gctacctgac gaatgtcaca aaccgggcgc
     4261 tctgcgcgta gcgtgcgaac ccgccgaatc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn ntcggatcgg gcgcttggat gtggcaagag ctggtttgat gggcaagtca
     4441 gtttcctccg ggctgccaga tccctgtcag tggaagggtt gtcgagagcc cggaggaaac
     4501 agcaataaag cggagagggg gggattcgaa cccccggtac ggttacccgc acactggttt
     4561 tcgaaaccag cccgatcagc cactccggca cctctccgac ccacgttcag caggctgcaa
     4621 cgggcttgag cgcggatcat ccacgacttg cacgcttctg tcaatctgtg gcgcggttgc
     4681 gcagcgcctc gtggccctcg tgttcgaatt gcattgtggc tcagattagg ttcggtcctc
     4741 gctggcgctt cgggctcgga tcgcgctgaa tactcagtaa actcgtgagc tgaatactcg
     4801 gtaagctgag tttagtctag gccctttgcg ccgatctcgc cgtttccggg ccgatcttgc
     4861 cgtgctgagc tgggcccttt cgccttccgc gccggccatc gctcgcgggc tggcttgaca
     4921 ttcgcgcgca gcgcgcggaa gctacttatc tgcggcggga gcgggcatgg ccgctgggac
     4981 tttgaacttc attttcgctg gcctgattgg gccggaagga gcgacgtatg gcgattcgtg
     5041 tcgcgattaa tgggtttggg cgaatcgggc gcttggtcat gcgagctggc atcaaggacc
     5101 cgcagcttga gtttgtggcg gtcaacgacc tgttcccgcc ggcttcgctg gcttacctct
     5161 tcaagtacga ctcgacgcac gggaagtggc cggggcaggt tgaggcgacc gaggcggcgc
     5221 tgcgcattga cggcaaggag atcagggtct gcggcgaaaa agagccggcc aagctgccgt
     5281 ggaaagacct gaaaatcgat tacgtgattg agtcgaccgg tctgtttacg gcgcgcgagg
     5341 gcgccagcca gcaccttaag gccggggcca agcgcgtcgt tatcagtgct ccggcaaagg
     5401 acaaggacat tccaacgttc gtcctgggtg tgaacgaaga gaagtacaac ccgcagacgg
     5461 acacgatcct ctcgaacgcg agttgcacga ccaactgcct ggccccgatg gccaagctga
     5521 tcaacgataa ctttgggctc gaagaaggtt tgatgacgac catccacgcg gtgacggcca
     5581 cgcagccaac cgtggacggg ccttcgaaga aagacctgcg cggcgggcgt ggtgccgggc
     5641 agaacatcat tccggccagc accggggcgg cgaaagcggt tacgctgtgc atcccagagc
     5701 ttaagggcaa gctcacgggc atggccttcc gcgtgccgac gcccgacgtt tcgtgcgtgg
     5761 acctgacctg ccggaccgcc aaagagacat ctatgtccca gataaactcg gtgttcaagg
     5821 cggccgctga aggcaagatg aagggcatcc tgggctacac cgaggagccg gtggtcagct
     5881 cggatttcat aagttcagct ttttcatgcg tttacgacgc aaccgcggga atggaactga
     5941 atccgcgctt cttcaaactg gtgggatggt acgataacga atacggctat tccaaccggg
     6001 tggtcgacct gctgaagtac ataggaaagc gggatggatt gatttagacc cgcaaaacaa
     6061 taggatatga ggtttcacgc ggcccgtccg ttccctggat gggccgtgtt gccttttggg
     6121 ctgtgccggc tgttgccggt agaacagcgc ggagaagcgc gatgaacaag aaatcggtgc
     6181 gtgatctcaa cgtgtccggg cggcgcgttc tgatccgggc tgacctgaac gtcccgctcg
     6241 ataagaatcg caatgtaact gatgaccggc ggatcagaat gttcctgccg acacttcagc
     6301 acgtgctcaa aggcggcggc cgggcgatcg taattagcca tctgggccgg cccagcggca
     6361 acccggctga agaccaggcg ttttcgctgc ggccggttgc cgccaagatg gagcagctcc
     6421 tgggacgacc cgtcaaattc gcgagcgact gtatcggccc ggaggcggac gcggccgtgc
     6481 aagggctgaa agacggcgaa gtgttgttgc tggagaatct ccgttttcat aaggccgaga
     6541 cggtcatcga caagaagaag aaaaacccgg acggcaaact cacgccggag caagaagcgg
     6601 cgcggcagaa attcgcccag gcgctggcgc ctcacggcga gatttacgtg aacgacgcgt
     6661 tcggcacgtg ccatcgccag cacgtgagca tgtacgacgt gccgctgctg acgcccaaag
     6721 gcaaccgggt ggtgggtttt ctggtggaaa aggagttgca gtacctgggt caggcgctgc
     6781 aatcgccgaa acggccgttc gtggcgatac tgggcggggc gaaagtcagc gacaagatcg
     6841 gcgtgatcaa gaatttgctg cagcgagtgg atcagattct gatcgggggg gcgatgacgt
     6901 acacgttctg ggcggcgcag ggcaagtcgg tcggcaagag cttgtgcgag cgggacaagc
     6961 tggatttggc aaagagcctg ttggagcaag gcggcggcaa gatcgtgctg cccgttgact
     7021 cggtcgccgc ggcggagctg aaggccggtg ttgcgacgca ggtgatcgcg ggcgacctgc
     7081 cggcagacat gatggggctg gacattgggc cgaagacgat cgaactgttc cgcaagtaca
     7141 ttcagcaagc gggcacgatc gtgtggaatg gtccggtggg cgcgtttgag acgccgccgt
     7201 ttgagaaggg cacgtttgag ctggcgcggg ccattgccga ggcgaccgat cgcggcgcgg
     7261 tgacggtcat cggcggcggg gacagcgcgg cggccgtcga acaggccggg ctggcggagc
     7321 gcatgtcgca catcagcacc ggcggcgggg catcgctcga attcctggaa ggcaaaccgt
     7381 ttaagacgat tgaagtgctc gacgatgcct gaaggtgagc gacgcaatcc gcgcatgaca
     7441 accattcgca tcgctccgtc ggtgctcagc gccgatttcg gctacctggc ggagcagatc
     7501 gcggtagtcg agcgcgccgg ggccgacctg ctccacttgg acatcatgga tgggcacttc
     7561 gtgccgaatt tgtcgctggg cgtgccggtg gtcgccagca tccggcggca cacgggcatg
     7621 tttctcgacg ctcatttaat gatcaccgac ccgggccggt atgcgccggc gttcgtcaag
     7681 gccggtgcgg acagcatcac gtttcatatt gaagttgctg agcggccgcg cgaactgatt
     7741 aagcagattc gtgacttagg cgtgaaagtt ggcgtgtcgc tgaatcctgg cacgccggcc
     7801 gagcgaatat tcgacattct cgacttggtg gacctcgtgc tggttatgac agtctggccg
     7861 ggatttggcg ggcaggagtt catgcgcgag tgtctaccga agattgaggc cattgcggcg
     7921 cggctgaacg cgaatcaatg gcttcaagtg gatgggggcg tggataataa gacggccccg
     7981 ttggttgtgg ccgccggcgc agacacgctg gtggcgggct cggcgatctt tgggcatgct
     8041 gacccggggt cggcggtaat tgaattgcga cgtgcggcgc aggcatccgc acggtctggt
     8101 acggaaacgc ggttatgaaa gtcgtcctga ttccctgtgc cgcaacggag tggcgggagg
     8161 aaggtcgcct gttgggacgc gtggagttgt ccccgtgcac ggatgcacgg caggaatgtg
     8221 aagaatgggc cgccgagctg caagccgccg gcctggcaca gatttaccat ggtccggatg
     8281 agctgtcgca cgagaccgcg cgttggctgg cacgctggct gacggtgccg gcgaagcgcg
     8341 ccgaccaact ggcggaagtg gatttgggct tgtgggccgg cctgacggat cagcagctca
     8401 agtcgcgcta tgcgtcggca catcgcgagc tgcgcgaagc gccgctgaac gtaaacccgc
     8461 cggagggcga gaacctgggc gacgccgaag agcgcttgcg ggcgttcgtg aagaaacaat
     8521 tgaagcgcaa cggcaaagcc gccgtgggtt tggtgctgcg gccgctttca tttgcgatgc
     8581 tatggcggag cctggcgggc cagcaagcgc cggaggtttg ggaagggatg cggcactggc
     8641 cgcgcccggt ggttattgat tgttcatcgg agcagccggt attctctggt gactgatttg
     8701 ggttcgcgat atgggccggt cgcggcgtgg gggcgagtgg ccaaagaaac gcacgggctg
     8761 agacggcccg ggccagcttg gagggcgtat gggcgagtcg ccgattcctg tgcggcgtaa
     8821 cgtggatgtg ccggaggggt tgtggattcg ctgcgtcaaa tgcggagcga tggtctaccg
     8881 gcggattctc gaagaggagc tgcgggtatg cccggagtgc gactatcact atcgcatcga
     8941 cgcgcgcacg cgcctgcaac agctcaatga ccccggcaca tttgaggaat ttctgcccga
     9001 tctggaatcc accgatccgc tccagttcac cgaccgcatc ggctacaagg accggcttga
     9061 ggaggccaaa gccgcctcga acgaacgcga ggcgattgtc gtcggcaagg gcttcatcaa
     9121 aggccgcccg gtgatcatgg gcgttatgaa tcccgatttc atcatgggct ccatgggggc
     9181 ggtggttggc gaaaaggtgg ccgcggccgc cgagcgggcg atcgaagagg aactgccgct
     9241 gatcatggtg acctgcagcg ggggggcgcg catgatggag ggcatggtct cgctggtgca
     9301 gatggccaag acttcggcgg ccattgccaa gctggatgcg gcgggcgggc tgtacgtggt
     9361 ggtgatgacg gatccgacca cggccggcgt ggcggccagc tttgcgtttc tgggcgacgt
     9421 tacgttggcg gagcccgggg cgcagatcgg tttcgccggg ccgcgcgtga tcaagaacac
     9481 gatcaaggct gagctgccgg agggcttcca gacggcggag ttcatgctcg agcacggttt
     9541 cgtagaccgg atcgtgcacc gcaaggacct gcgaagcgaa atcgcgaggg tgattgatta
     9601 ctgcaacaag tgagagcggc gccggcgcga gcgcggaacc cggtaccgcg tgtgccactg
     9661 ctcttgagca gtgttcttcc cgtttctccg ctggcctgag gccctgatgc tgccgtcctg
     9721 gtttctggaa aagctgctcg acccgctggg cctgttcggt ctagcggcgc aatcgtgctt
     9781 catgctgcgt ttcgtcgtgc agtggttcgt ttccgaacgc cgcgggcgca gttacgtccc
     9841 ggtcgcgttc tggtacttga gcctggtcgg cggggcgatg accttcatct acgcggtgct
     9901 acgccaggat ccggtatttg cactggctca ggcgctaggg ctggcgatct atacgcgaaa
     9961 tctggtgctg atctaccgcc gccgctcgcg aaccgccgcg cgccacccgc gctccgacgc
    10021 cgaagtggcg cgcgccggcg caggagcaat gccgaacgcc gattgagacg aagcagcgcg
    10081 ggagacctgc gccggccacc gacgagtacc ttcagctccc ctgcgggaca acggactcga
    10141 atagtacccc gcgaagtggc cacgcagcgg tgatggaggt cagccgtacg actagggcgg
    10201 cgtgcggctc gactgggttt gggctgacga gaacccattc gccgttgcgg cggaacacgc
    10261 gctttatcac gttgccaata gtttcgtcac tggtctccag cacggcgagg ccacctggct
    10321 cgagcgtggt ctcacccaca ggctgcttgg cggacacaag cacatgctgg ccgcgacggg
    10381 cgagcggtgc gaggctatca ccctcgacag tgatggctcg gagagttggc aggcaggctg
    10441 catcgaaatc tgcccggggc tgccactcgt gcggctcggc tgaagctcta gtaccagggg
    10501 accgaagcgg ctcatacagc acgccacaga ttctgcggac cgctgtcgaa gctcgaggaa
    10561 cgataatcgg tttggttggc tgcaccgggt tcgcggattc aaggtgcaag tccgacccgt
    10621 ttatccaaac gcggcgaagc agccttcgtc cacctgcgtc ggcgacgacg aggtctccat
    10681 cccgcactga cgtttccgca cctgcaatta gcacccattg tcccgggcgg ataatcggat
    10741 ccaccgtgtt cgcaagcgca agcgccgctc cacccggcgg cactgccagc gtcgctgcat
    10801 cttcggtcac gtcgcaagcc caggtgtctt gctttgcggc agcacgtccg agcacggcga
    10861 tgctgattgg gccgtcccac tgcgcaccgt gcggaaaacg aactgggtcc tcaggcacgg
    10921 ccggcaacgc cctgccctgc cgctcatgag ttctgatgcg ctccaagcgt tccagttcgt
    10981 cctgcaatct gcagttacac tgtcggagct tctccaaaca gcgtctgatc attgaaactg
    11041 ttacgtctgc ctcggtgagg tgatgcgagc cggggtcaag cactcgggtc acttccgggt
    11101 cagttagcag agccacgatc gccccggctg aagggctgga tgtgatccga gagttcgtcc
    11161 gccgcataga ccagagcagg gactcggttg tttcgtttaa ctgggatgtt cttcttgaga
    11221 tcgccctggc gaacgggtct cggccatcaa gcccgccaga attgggatta gggtccgatg
    11281 ggcgattccc gatgcgtgaa cgcattgtaa agggggccgg gtggagaagc aatggtacgg
    11341 ggaggtgtcg gcagtcgggg ccggcgttag tagtaagtcg ttagttttta gttgttagtt
    11401 gttagacgtt agtcgttagt tgttagtagg gaacaaaggc gcgagagcgt tggccggtag
    11461 tggagccgtc gtcaggggag atgcgcggcg cgcaaccgta gtcgccagcg gcagacgacg
    11521 gctggggcag aaggcacaac gtggcggcgg tggttatcag cggcggccgc tgtcagtcgc
    11581 gggggcggcc gccgttaccg ccgtcgttaa gtattccccg cgatccagcg ttcgaggttc
    11641 tcgcgcgcta ttgaatctcg gaattgctgc ggcgggtgct tcattgtgac ggccgaaatt
    11701 tcgaccagag ggccgccgat gccgcggtcc agggccagct ttgcacagcg gatggcgtcg
    11761 acggccatgc cgccgctgtt gggcgaatcc tccacgctga gtcgcaattc caggttcatg
    11821 ggcacgtcgc cgaagcccag gtactccatc cgcaggaagc agaccttgtt gtccttttgc
    11881 caagggacgt aatcggacgg gccgacgtgg atattgatcg gctccagcgg cgtatcaagc
    11941 tgcgactgga cggactcggt tttcgaaatc cgcttcgatt tcagccgcga ttgcgaaagc
    12001 atgttgagga aatcggtatt tccgccggta ttaagctgat aggtgcgggc cagcttcacg
    12061 ccgcggtcgc acatcaggcg cgccagggcg cggtgcagga tcgtggcccc gaactggctc
    12121 ttgatgtcgt cgcccacgac cgggatgttg cggcgacgga actcttccgc ccactccggg
    12181 tgcgaggcga tgaacaccgg gatgcagttc accatggccg cgccggcgtc gaggcatgcg
    12241 cgggcgtagt ggcgggcggc atcttcggaa ccgaccggca tgtagcagat cagcacgtcg
    12301 gtgcgtgact cgcgcagcac gcgcgccacg tcgcaaggcg gagcgtcagc cacccgaaag
    12361 gcctggtctt ccgggtactc gctcatgtga gcggcgacgc cgtccagcac cggccccatc
    12421 tga
//
LOCUS       DAOSYS010000093        12321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4357_length_12321_cov_5.538480, whole genome shotgun sequence.
ACCESSION   DAOSYS010000093 DAOSYS010000000
VERSION     DAOSYS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4357_length_12321_cov_5.538480"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLU99_07460"
     CDS             <1..412
                     /locus_tag="PLU99_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16930.1"
                     /translation="GREALLSHVTELLAYTAHYWSARVDRVMLSARNGAMSGLIFGAL
                     GVLGLATAIVCIVLLLQGFAGGLGAALGGNLWGGQLIVGGGLLLLMGVTVLIVLAAMK
                     AKLRRARVEKYETIKRNERARIGRDVDSAARAAH"
     gene            381..839
                     /locus_tag="PLU99_07465"
     CDS             381..839
                     /locus_tag="PLU99_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16931.1"
                     /translation="MSIRPPEQLTEAELLDQEIRAAKRAMQQARAQLKEDARRLVDPR
                     LWIQQYPVASTLVACFGGALLARGVRKKARHRVEVEPEVVELGKKAAQKSPFVRDVGA
                     ALRKSLVGLVTSGLVSRAASAFQEQSQPEAAYSGFEGAPEPAPTDVGGQI"
     gene            1151..1834
                     /locus_tag="PLU99_07470"
     CDS             1151..1834
                     /locus_tag="PLU99_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO16932.1"
                     /translation="MRFLVIEDNAKLADVIRKGLTEQAYGVDVAHSGHEGEDLAASKS
                     YDGIILDLMLPDHDGVALCRNLRRHGIATPILMLTALSSTGDKVTGLEAGADDYLTKP
                     FDLEELIARVRALLRRHQPAEGGTLRFSELEMDLVSRRVTRRGQPINLTAKEFALLEY
                     FMRNPNRVLSRSAIGERVWDMAFEEESNVIEVYVSRVRGKIDKGFDKPLIHTIIGTGY
                     VLSEDGPPG"
     gene            1956..5069
                     /locus_tag="PLU99_07475"
     CDS             1956..5069
                     /locus_tag="PLU99_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOO16933.1"
                     /translation="MLYKRYSRALRRAAGLNFKKLRWSMRRWIIIGTVTLAVVLLLGF
                     VSVWTHFGRRKTAELENWIGRQIVAVVQNYVHPRIAFAALHYQPPRTVLISGFSLSAG
                     AHTILSVDRTSLQLAEVPHRGKPIQVQRVEFERPKLLFLSEEQGGLVGWTDFLNREAV
                     RRPDTVPAGRRFSDILVLRHVEIHNGEVTYDDAGDDSAPMTLPGVDLTLDTPPDAESP
                     GWYRLMGTLQRAPVFRLQLDGRINLDTALLEFAKLNLQLALGEEQYHTLPPALQRLLR
                     SHAVQGALTAEAEGQIPLRKSGESQVQVRARLEDGTFAYGGAAFPVKSVEVDAQLPKG
                     PIHLSASAFAVSAGGQTIASADGIALDLPTLPGAGEPLRIEQLQLDSPRLAFWRDGKG
                     GYAGWSDLRSSSARRGPRMAADAERTARPAAETVQRILEKVSLGALQVRNGGVLYEDR
                     AGKPLVVEGFNATLHPSRAPNAPETWQLEGTFDVPSFLTSQFDGRFNADTLDLDIERL
                     ELKAELTEAGYASLSPRMRKSLPAEQLFGGLTAGVQVHVPLAAPAQATAQAQVEVTDG
                     YVVYRDTVWPFARLSAAGSWPEGPVNITGENLQMRGREESVLTVPRLALELPYVPRPG
                     QPLEISRLVLDSPQVELREVPGGGFAGWNELLGKEQQPPAPRPPASEGQESGAHWRLR
                     SLEARDGALRLASLASPYMMSIRGINVALASAPNAGWYDLNGTVRQAPALDMTFAGVL
                     NPTQSLLELSDLRLTAEFGENRYAILPPKIQTIVRGRDVRGTLQAHVTGLLAWRDPQR
                     TQARVSAQVNNGHVALGRLEWPIQTLQVTSQIRAGEVAADYAGTLLGGEVSGSAQLAL
                     GPPKTFTLNWNAAGLQLEQMLRVAEGRRPSHAGRIVSTGTISWQAGAWPASLSGSGTL
                     RITQGRLIELPVIRDVLALIARTRLGSRLTSMDSADATFSIHPDYVYLSRADIITVLA
                     ALYGRGRVYYDKRLDLDVRASPLGELQRLTGRIGEALGRLTGDIITYKVQGTIARPVI
                     RPVPLGL"
     gene            complement(5097..7151)
                     /locus_tag="PLU99_07480"
     CDS             complement(5097..7151)
                     /locus_tag="PLU99_07480"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOO16934.1"
                     /translation="MRYVPALLLLIGMTLLAACAASDKRIVVESSCTEEPVEFSTPPV
                     CTKTLLQEMIDLCGLPEYPDPPYIVAQFSSYDRAARSPADNWFANEDAGHFLRIEEYP
                     DRTEYVMMDAEGPGAVVRLWSANPDGILRMYIDGNPRPALEAPFRDLLSGKVTAIPEP
                     LSGEASSGWNCYFPFPYARHCKITTDKGEGLYYHIDYRTYAPETRVVSFHPGDLDMNR
                     LSIHLLASQLSAPRRAHLTALCGCEVRTARKHIRLGPGCAEQMAFRSGPCGCAVYMFH
                     AKARCENIEQGLRDLVLSICFDGTQTVLCPLGDFFGAAPGLNPYESLVMGVDCDGTMW
                     SHWVMPFQRSAVFEIRNLGACPASLDLGVSTGSYEWSDRTMYFHSQWRAARDVATRPM
                     QDWNYAMLTGQGVFAGAAFNICNPVKQWWGEGDEKIYIDGESFPSIFGTGTEDFYGYA
                     WGSPIPFAHAYHCQPCCSGPANYGNTAVNRWHILDRIPFRYSFRFDMELWHWWEGVIP
                     GMSVATYWYGRPGATGNPMAPTPDLLKVCRMPPYEPMRVAGALECEELRLVATGGEFT
                     PQEREGCSNDRHLWWRGGLADRLVLAFDVPETCRYRVLVSGLMAPDYAITQFYVNDDP
                     VGEPVDFYSPAANVGQELLLGAFDLPCGENLLTIELIGANEAGLRSYMVGLDYLRLEP
                     VH"
     gene            complement(7304..8086)
                     /locus_tag="PLU99_07485"
     CDS             complement(7304..8086)
                     /locus_tag="PLU99_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOO16935.1"
                     /translation="MPVTFRRVQLAGLTLHAATMHDALTLADQAIRQRRRLAFGMVNI
                     AKLVNAGRDPLLQDSLLKSDVVLADGQPVVWLSRIQGEPLPERVAGIDLMYRLLELAN
                     QRGHAVFLLGATEAVNAAVAARIQQCYPACRLAGRRDGYFSEAEEAEVARQIRDSGAD
                     ILFVAMSPPRKELFLRRWSDEMGIPVCHGVGGSFDVFAGLTRRAPLWMQRAGLEWLYR
                     IWQEPRRMWKRYLVTNTKAIPLLFAALIARCRRLPAAHPPAQ"
     gene            complement(8134..9369)
                     /locus_tag="PLU99_07490"
     CDS             complement(8134..9369)
                     /locus_tag="PLU99_07490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO16936.1"
                     /translation="MTHATVAAEEATRRAPQKTPRYDGVVCFALGDWWYHNRGHFDMQ
                     LMKRFAQRVPVLYVNAMGMRVPSMRERSVFITRIRRKLRSLMRGLTRWDERFSVLSPV
                     SFPAYSRPWWRRVNAASVRLQVRHALRRVGLRRPLVWVVSPPAVDIACRLPRAALVYQ
                     RTDDFKHFTGVNQDVIAGMDRQLVDRADLVIHVNRGLYEESLAHGARALLTSHGVDYD
                     LFAPEPDQRGEPEDIAAVPHPRIGFYGGIDNHTFDPDLLLAVAAALPDMHFVLVGACS
                     LPPGTFATAPNVHPLGQKPYEQIPAYGRAFEVAIMPWRKNDWIAACNPVKLKEYLALG
                     KPVVSTPYPEGAQYRDCVYFAATAGEFADAIRRALCEDDAEKRAARRQRVAGQTWDAL
                     ALTLLAEIDDIVAEKDLSQ"
     gene            complement(9366..10412)
                     /locus_tag="PLU99_07495"
     CDS             complement(9366..10412)
                     /locus_tag="PLU99_07495"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOO16937.1"
                     /translation="MNEAHCETVEIGRWRELAATFADYNYRHVWAFGQACAARRGAVS
                     EHVAVTCGAEVLALADVRIKRTPLCRTGIAYINGGPLVRRGAPDDIQALARALEALRR
                     TYVEKRRLLLRVAPSPGPPLWNEQLQACYISAGFKQSSTVRNSTILIDLAPEPDELRK
                     GLHAKWRNDLTHAERAGLALRTGRDDELLAAFCRLYDELIERKEFAVDLDPAFYARVQ
                     ANLAEDEKFEITLAESNGRPVAGHVAALHGDTAVYLLGASNADGLKTRASFLLQWDVL
                     TRARSRGYRWYDLGGIDPENNPGVYRFKRRMGGVEVTAAGPFEVAPGELQRLIVSGSE
                     RIYRAMRSRVGAGK"
     gene            complement(10409..11137)
                     /locus_tag="PLU99_07500"
     CDS             complement(10409..11137)
                     /locus_tag="PLU99_07500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOO16938.1"
                     /translation="MRTDQLQRFTWQMRHTARRAVSPSAVASGRRASGGSPYICVTFD
                     DGFENVLKNALPAMQALGVPATLFVVPGSFGMVPAWGMPVGHPDREERVASGPELAAA
                     ARRGLCSIGSHTFSHVRLSQAKRAEMMRELSDSKEALEELLDCEIVDLAFPYGDYDAT
                     TVERAYSSGYRRLFTLDPRLWHPEKGETLVGRFLVCPEMWRVEFALTCAGAYAWRAAW
                     RRVWQRLRPARQRRAAGAPEVVAT"
     gene            complement(11289..>12321)
                     /locus_tag="PLU99_07505"
     CDS             complement(11289..>12321)
                     /locus_tag="PLU99_07505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO16939.1"
                     /translation="LCVFVFRAAGELVAILPMFIDRLRLGPVTVRLAKFIGSDSTLAV
                     LNPPVEPDQARQVYADVLRQLLYVHKCDAVSFGPLSGERPHARTVRAACQGSPGTYVV
                     RDQATTVHNVYTLPADSDTYLRGLQKTQRREYERGWNRLTTKHQVAVESVSGDAAVPA
                     YEAFVSLHTSQWRRARKLGHFHDWPGALEFNRDLVRTLAAENRAGFCNLRADGELVSS
                     NYFLRFGTHEHWRLSARDVRPQWNRAHLGILVQVRAVEAAIAAGVRRAEDGPGRYDYK
                     TAMGALEYPLQSIVVARGKIGRVKTRLLSVVSDALHLLYYQIWFGRIAPCLPLPRRGL
                     WTLWTRYRV"
BASE COUNT         2284 a         3718 c         4082 g         2237 t
ORIGIN      
        1 ggggcgcgag gcgctgctaa gtcacgtgac ggagctgctg gcctacaccg cccactattg
       61 gtcagcgcgt gtcgatcgcg tgatgctgtc ggcgcgcaac ggcgccatgt cggggctgat
      121 cttcggcgcg ctgggggttc tgggtttggc gaccgcgata gtttgtatcg ttcttctgct
      181 gcagggcttt gccggcggat tgggcgcggc gctgggcgga aatctgtggg gaggtcagct
      241 aatagtggga ggaggactcc tgctgctgat gggcgtcacg gttctgatag ttttggctgc
      301 gatgaaggcg aagctgcggc gcgccagggt tgagaagtat gaaacaatca agcggaacga
      361 gcgggcacga atcggacgag atgtcgattc ggccgcccga gcagctcact gaagctgagc
      421 tcctggacca ggaaatccgt gcggccaagc gggccatgca gcaggcgcgg gcgcagttga
      481 aagaggacgc gcgtcgcctg gtggacccac ggttgtggat tcagcaatac ccggtggctt
      541 caacgctggt cgcctgcttc ggcggagcgc tgctggcgcg gggagtacgg aagaaagcgc
      601 ggcatcgggt ggaagttgag ccggaggttg tggagctggg aaagaaagcg gctcaaaagt
      661 cgccttttgt acgagacgtc ggagccgcgt tgcggaagag cctggtcggc ttggtcacca
      721 gcgggttagt gtcgcgagcg gcgagcgcgt ttcaagagca atcccagccc gaggctgcct
      781 attccggttt tgagggcgcc cctgagccgg ccccgaccga cgtgggcggc cagatctgac
      841 gaggttgcac ggcggcatga gcggcaagca acgtcgcgga cgaaggcagc cctgaacgta
      901 acaatcaacc gcggaggcga aaccggcaat tgcataatcg agacgggatt aaatcgcagt
      961 cacctcatca ggaggaaggt tgttggacgc tggtgccttg acggcatcgg cgcgaaacga
     1021 gaaactaggg tcagtctgcg gaggtgagtt tcaggggaac gactcaagcc tcgcaccgca
     1081 gcatacggtt cgtgccgaac taagccgagg acggcgtgca tgaggcagag gaagccctta
     1141 aggaggccgc atgcgctttc tcgtcattga agataatgcc aagttagccg acgtgatccg
     1201 caagggtctg accgagcagg cctacggggt ggatgtggcg cactcggggc acgagggtga
     1261 ggacctggcc gccagcaagt cgtatgacgg catcatactg gacctcatgc tgcccgatca
     1321 cgacggcgtg gcgttgtgcc gaaatctacg gcggcatggg atcgcgacgc cgattctgat
     1381 gctgacggcc ttgtcctcga cgggcgacaa ggttacgggg ctggaggcgg gggctgacga
     1441 ttacctcacc aagccctttg acctcgaaga gttgatcgcg cgtgtgcgcg cgttgctgcg
     1501 ccgccaccag ccggcggaag gcggcacgct gcgtttttcg gaactggaga tggacctggt
     1561 cagccggcgc gtcacgcggc gcggccagcc gatcaatctg acggccaagg aattcgcgtt
     1621 gctggagtac ttcatgcgca accccaatcg cgttttgagc cgatcggcta tcggcgaacg
     1681 cgtatgggac atggcgtttg aggaagaaag caacgtgatc gaggtttacg tctcgcgcgt
     1741 ccgcggcaag atcgacaagg gtttcgacaa gccgcttatc catacgatca ttgggacggg
     1801 ctacgtgctc agcgaggatg ggccgccagg gtgatgacgc gcggatcggc gggcccgcga
     1861 accgtagcgg ttcgccctga gttgcgattc aagtttccat aacatcgcct cacgcgcttt
     1921 aaagccggga caacgcggcg accagcgata attgagtgct gtacaagcga tacagccggg
     1981 ccttgcgccg agccgcgggc ctgaacttca agaagctgcg gtggagcatg aggcgctgga
     2041 taatcatcgg tacggtcacc ctcgcagtcg tgttgctgct cggttttgta agcgtctgga
     2101 cccacttcgg gcggcgcaaa acggccgaat tggagaactg gattgggcgg cagatcgtgg
     2161 cggtcgtgca gaattacgtg cacccgcgga tcgcatttgc cgctctgcac tatcagccgc
     2221 cccgcacagt gttgatttcc ggcttttcac tgagcgccgg cgcgcacacc attctgtcgg
     2281 tcgaccgcac gtcgctgcag ttggcggaag tgccgcaccg cggcaagccg attcaggtgc
     2341 agcgggtgga attcgagcgg ccgaagttgc tgtttttgtc ggaggaacag ggggggctgg
     2401 tgggctggac ggattttcta aaccgcgagg ccgtgcggcg gccggatacg gtgccggcgg
     2461 ggcggcgctt cagcgacatt ctggtcttgc ggcacgtaga aatacacaat ggcgaagtca
     2521 cgtacgacga cgcgggggac gacagcgcgc ccatgacgct gccgggggtt gacctgacgc
     2581 ttgacacgcc gccagacgca gaaagccccg gctggtatcg gctgatgggc acgctgcaac
     2641 gggcgccggt gttccgcctt caactggacg ggcgaataaa tctggatacc gcgctcctgg
     2701 agttcgccaa gctcaatttg cagttggcgc tcggcgaaga gcaatatcat acactgccgc
     2761 cggcactgca gagacttctg cggtcgcacg cggtgcaggg ggcgctcacg gctgaagccg
     2821 aggggcaaat cccgctgcgc aagtcggggg aatcacaagt tcaggtgcgg gcgcgcttgg
     2881 aggatgggac gttcgcgtac ggcggggcgg ctttcccggt caagtccgtt gaagttgatg
     2941 cacaattgcc gaaaggcccg attcacctgt cggcgtctgc gtttgcagta tcggcgggcg
     3001 ggcagacaat cgcgtcggcc gatggcatcg cgcttgatct gccgacgttg cccggcgcag
     3061 gcgaaccgct gcgcatcgag caactgcagc tcgattcgcc gcgtctggcg ttttggcgcg
     3121 acggcaaggg cggctacgcc ggctggagcg atttacgcag cagttccgcg cggcgcggtc
     3181 ccagaatggc ggcggatgct gaacgtactg cacgcccggc ggcggaaacc gtgcagagaa
     3241 tcttggaaaa ggtgtcgctg ggggcgttac aggtgcgcaa tggcggcgtt ctctatgaag
     3301 accgggccgg aaagccgctg gtcgtagagg gcttcaacgc gaccctgcat ccttcacgcg
     3361 cgccgaacgc acctgagacg tggcagcttg agggtacgtt tgatgtgcct tcttttctga
     3421 cgagccagtt tgacgggcgc ttcaatgcgg acacgctcga tctagacatt gagcgcctgg
     3481 agttgaaggc cgagttgaca gaggccggct atgcttcact ctcgccccgc atgcggaaga
     3541 gcttgccggc agagcaactg ttcggaggct tgacggccgg cgtgcaggta cacgttccat
     3601 tagctgcgcc ggcgcaggcc acggcacagg cacaagtcga ggtcaccgat gggtacgtag
     3661 tctaccgcga caccgtctgg ccgttcgcgc gtttgagcgc ggcaggaagc tggcctgagg
     3721 gaccggtcaa cattaccggc gagaacctgc aaatgcgtgg ccgcgaagaa tcggtcttga
     3781 ccgtaccgcg tctggcactg gaactgccgt acgtgcctcg accggggcag ccgctggaga
     3841 tctcgcggct ggttctggac tctccacaag ttgagttgcg ggaagtcccg gggggcgggt
     3901 ttgccggctg gaacgaactg ctcggtaagg agcagcagcc gcctgcgccg cggccgccgg
     3961 cgtcagaggg tcaggagagc ggggcgcact ggcggctgcg tagtttggag gcgcgcgacg
     4021 gcgcactgcg cctggcgtcg cttgcttcgc cttacatgat gtctatcagg ggcattaatg
     4081 tagcgttggc atcggcccca aacgccggct ggtacgacct caacggcacg gtgcgacagg
     4141 ccccggcttt ggacatgaca ttcgcgggcg tactgaatcc cacgcagtca ttgctggagt
     4201 tgtccgatct cagactcacg gcagagttcg gtgagaatcg atacgccatt ttgccgccca
     4261 agatacaaac gattgttcgc ggccgagacg tgcgcgggac gctccaggcg cacgtgacgg
     4321 ggctgctggc gtggcgcgat ccgcaacgca cacaagcacg tgtgtcagca caggtcaaca
     4381 atgggcacgt tgccctggga cgtcttgagt ggcccataca gacgctgcaa gtaacaagcc
     4441 agattcgtgc aggagaggtc gcggctgatt atgcgggcac actgctggga ggcgaagtga
     4501 gcgggagcgc acaactggcc ctgggtccgc ccaagacctt tactcttaat tggaatgccg
     4561 ccggattgca gttggaacaa atgttgcgcg tggctgaggg ccgccggccc agccacgccg
     4621 ggcggattgt ttcgaccggc acaatttcgt ggcaggccgg ggcatggccg gcttctttgt
     4681 caggcagcgg aacgttgcga atcacacagg gccggttgat agaactgccg gtaattcgcg
     4741 atgtgctggc gctaattgcg cgtacgcgcc tagggtcgcg cctgacaagc atggacagcg
     4801 ccgacgccac cttcagcatt cacccggact atgtgtattt gtcgcgggcc gacatcataa
     4861 cggtcttggc ggcactgtac ggtcgcggcc gagtgtacta tgacaagcgc cttgatctgg
     4921 acgtccgggc cagcccgctg ggcgagttgc aaagacttac cggccgtatc ggcgaggctc
     4981 tgggacggtt gacgggcgac atcattacat acaaggtcca aggcactatc gcgcgcccgg
     5041 tgattcgccc ggttccactg ggtctctgat ttgtggggtg tctgcgcgcc ctcagttcag
     5101 tgtaccggtt ccagccgcag ataatccagg ccgaccatgt agctgcgcag ccccgcctca
     5161 ttggcgccga tcagctcgat agtcagcagg ttttcgccgc acggcaggtc gaaggcgccc
     5221 agcaacagct cctgcccgac attcgcggcc gggctataga aatccaccgg ctcgcccacc
     5281 ggatcgtcat tcacgtagaa ctgcgtgatg gcataatccg gcgccatcag gccggatacc
     5341 agcactctat agcggcacgt ttcgggcaca tcgaaggcga gcaccagccg gtcggccagg
     5401 ccgccgcgcc accaaaggtg gcggtcgttg ctgcacccct cgcgctcctg cggggtgaat
     5461 tcgccgccgg tcgcgaccag acgcagttct tcgcattcca gcgctcccgc cacgcgcatc
     5521 ggttcgtagg gcggcatacg gcataccttc aacaggtcgg gcgttggggc catgggattg
     5581 ccggtagcac ccggccggcc ataccagtat gtggccaccg acatgccggg gatgacgccc
     5641 tcccaccaat gccatagttc catatcgaat cggaagctgt aacggaaggg tatgcggtcg
     5701 agaatatgcc agcggttgac ggcggtattg ccatagttgg ccgggccgct acagcacggt
     5761 tgacagtgat aggcgtgtgc gaaggggatc gggctgcccc aagcgtagcc gtagaagtcc
     5821 tcagtgcccg ttccgaagat gctggggaag ctctcaccgt cgatatatat cttttcgtcg
     5881 ccctcgcccc accactgctt gaccgggtta catatattga aggccgctcc ggcgaagacg
     5941 ccttggccgg tgagcatggc gtagttccaa tcctgcatgg ggcgcgtcgc gacgtcgcgt
     6001 gcggcgcgcc actgggaatg gaagtacatc gttcggtcgc tccattcgta cgacccggtg
     6061 cttacgccca ggtcgaggct tgccgggcac gcgcccaggt tgcggatctc gaagactgcg
     6121 gagcgctgga agggcatgac ccagtggctc cacatggtgc cgtcgcaatc gacgcccatc
     6181 accaatgatt cgtagggatt caggccgggg gccgcgccga agaagtcgcc cagggggcat
     6241 aggacggtct gcgtgccgtc gaagcagata ctcagcacga gatcacgcag gccttgttcg
     6301 atattctcgc agcgcgcctt ggcgtggaac atgtacaccg cacaaccgca ggggccgctt
     6361 ctgaaggcca tctgttccgc gcagccgggc cccaggcgga tgtgcttccg ggcggtgcgc
     6421 acttcgcagc cgcacagtgc ggtcagatgc gcccttcgcg gcgcggacaa ctgcgatgcc
     6481 agcaggtgaa tagacaacct gttcatgtcc aagtcgccgg ggtgaaagct gaccacgcgc
     6541 gtctcggggg cgtatgtgcg gtagtcgatg tggtaataca gcccttcgcc cttgtccgtc
     6601 gttatcttac agtgccgggc atacgggaac ggaaaatagc agttccaacc ggaactggcc
     6661 tcgcccgaga gcggctccgg aatggcggtg accttcccac tcaggaggtc acggaagggt
     6721 gcttccagcg ccgggcgcgg attgccgtcg atatacatcc gtaagattcc atctggattg
     6781 gccgaccaga ggcgcaccac cgcgccggga ccctctgcgt ccatcattac atactcggtg
     6841 cggtctgggt attcctcgat ccgcagaaag tggccggcgt cctcgttggc gaaccagttg
     6901 tccgccggcg accgagctgc ccggtcgtag ctggagaatt gcgccacaat gtacggcggg
     6961 tcgggatatt cgggcaggcc gcacaagtca atcatttcct gcaacagcgt tttggtacag
     7021 acaggcgggg tgctgaattc aacaggttcc tccgtacaac tggactccac gactatccgt
     7081 ttgtcggacg cggcacaggc ggccagcagg gtcataccga tcagcaacag aagtgcgggc
     7141 acgtaacgca tgttgtctct ccagctccgg atggcgcgct ccgaaccgcc gccgcgaacc
     7201 cggcgcgaca gaaagggcca ggattcggcc cgcgattcgt gctgcgcatg ctagccagac
     7261 ccggctggcg ctgcaaggtt gttaggcatg gtctagcggg agtttactgt gcaggcggat
     7321 gcgcggccgg gagtcggcga cagcgtgcaa tgagggccgc gaacaacagc ggaatagcct
     7381 ttgtattggt gaccagatag cgcttccaca ttcgccgcgg ttcttgccaa atccgataca
     7441 accactccag tccggccctt tgcatccaga gcggcgcgcg gcgcgtaagc cccgcgaata
     7501 catcgaagct cccgccgacg ccatggcaca ctggaattcc catttcgtct gaccagcgtc
     7561 gcagaaacaa ctcttttcgc ggcggagaca tggccacaaa tagaatgtcc gctccggagt
     7621 cacggatttg ccgtgcgact tccgcttcct cggcctcgga aaagtagcca tcgcgccggc
     7681 cggccaggcg gcaggcaggg tagcactgtt ggattctggc ggcgactgct gcgtttaccg
     7741 cctctgtcgc cccaagtagg aagacggcgt ggccgcgctg gttggctaac tcgagtaacc
     7801 gatacatcag atcgatgccg gcaacgcgtt cgggcagcgg ttcgccttgt atacggctaa
     7861 gccagacaac tggttgaccg tcggccagga cgacgtcgga tttcagcagg ctgtcctgca
     7921 ggagggggtc gcgccccgca tttacgagct tggcgatatt taccatgccg aaagccaggc
     7981 ggcgccgctg ccggatggcc tgatcggcga gggtgagggc atcgtgcatg gttgcggcgt
     8041 gcagagtgag accggcgagc tgcacgcgtc gaaaggtgac tggcatacgc tccagtgcgg
     8101 gccgagcggt gccgatgggg ctgagagaac gtatcattgt gaaaggtcct tttctgccac
     8161 gatgtcgtct atttcggcga gcagcgtaag ggccagggca tcccaagtct ggccggcgac
     8221 gcgttgccgc cgcgcggcac gcttctcggc gtcatcctcg cacagtgccc ggcgaatggc
     8281 atcagcaaac tcgcccgcag tggcggcgaa gtacacgcaa tcccggtatt gtgcaccctc
     8341 agggtacgga gtggagacga ccggcttgcc gagcgccaga tattctttca gtttgacggg
     8401 attacaggcg gcaatccagt cgttcttgcg ccatggcatg atcgccactt cgaaggcgcg
     8461 tccataagcc gggatttgct cgtacggctt ctggccaagc gggtgcacat tcggtgcggt
     8521 tgcgaaagtg ccgggcggca gactgcaggc gccgacaagc acgaaatgca tgtccggcag
     8581 tgccgccgct acggctagca acaagtcggg gtcgaaagtg tggttgtcta tgccgccgta
     8641 gaatcctatt cgtggatggg ggacagccgc aatgtcctcc ggctcgccgc gctggtccgg
     8701 ctcaggcgcg aacaagtcgt agtcgacgcc gtgactggtc agcagcgccc gcgctccgtg
     8761 agccagtgat tcctcgtaca ggccacgatt aacatggatg actaagtcgg cccggtcgac
     8821 cagttggcga tccatgcctg ctataacgtc ttgattcacg ccggtgaagt gcttgaagtc
     8881 gtcggttcgc tgataaacca aagcagcccg cggcagccgg cacgcgatgt ccaccgccgg
     8941 cggcgacact acccagacca gcgggcggcg tagaccgacg cggcgtagcg cgtggcgcac
     9001 ctgcagccgg acgctggccg cattcacccg gcgccaccac gggcgtgagt acgccggaaa
     9061 ggagaccggg ctgagaacgc tgaagcgttc gtcccagcgc gtcaggccgc gcatcaggct
     9121 gcggagcttt cgccggatgc gtgtgatgaa gacgctgcgt tcgcgcattg aaggcacgcg
     9181 catgcccatg gcattgacgt atagcactgg gacgcgctgc gcgaagcgct tcatgagctg
     9241 catatcaaaa tgcccgcggt tgtggtacca ccagtctccc aacgcgaagc agacgacgcc
     9301 gtcgtagcgc ggcgtctttt ggggggcgcg gcgcgtcgcc tcttcggccg caactgtggc
     9361 atgcgtcatt tgccggctcc cacacgcgaa cgcatagccc ggtaaatacg ttcgctgcca
     9421 gagacgatca ggcgctgcag ttcgcccggt gcaacttcaa aggggccggc ggcggtcacc
     9481 tccacgccgc ccatgcggcg cttgaagcgg tatacgcctg ggttattctc gggatcaatt
     9541 cccccgaggt cataccagcg atagccccgg ctgcgggcgc gtgtcagcac gtcccattgg
     9601 agcaggaacg atgcccgggt tttcaggccg tccgcgttgc tggcgcccag caggtatacg
     9661 gccgtgtcgc cgtgcagcgc ggcgacgtgt cccgctaccg gccggccgtt cgactcggcc
     9721 agcgtgatct caaatttctc gtcctcggcg aggttggctt gtacgcgggc gtagaaggcg
     9781 gggtccaggt cgaccgcgaa ctcttttctt tcgatcaact cgtcatacag ccggcagaag
     9841 gcagctagca gttcgtcgtc gcgtccggtg cgcagtgcca agccggctcg ctcggcatgg
     9901 gtcaggtcat tgcgccactt ggcgtgcagg cccttccgca gttcgtcggg ctcaggcgcc
     9961 aggtctatca atatggtact attgcgaact gtggagctct gcttgaagcc cgcgctgatg
    10021 taacaggcct gcagttgctc gttccacaag ggcggaccag ggctaggggc gacgcgcaac
    10081 agcagacgcc gcttttccac gtaagtgcgg cgcaaagcct caagcgcgcg ggctagcgcc
    10141 tggatgtcgt ccggcgcgcc gcgccgcacc agcggtccgc cgttgatgta tgctattccc
    10201 gtacggcaca ggggtgtgcg cttgatgcgg acgtcggcca gggctaacac ctcggcgccg
    10261 caggtcacgg ccacgtgctc gcttacggcg ccgcggcgcg ctgcgcaggc ctgcccaaac
    10321 gcccagacat ggcggtagtt atagtccgcg aacgtcgcag ccagttcgcg ccatcggccg
    10381 atttcgacgg tttcgcagtg cgcctcgttc atgttgccac cacctccggc gccccggcgg
    10441 cccgacgttg cctggcgggt cgcaagcgtt gccatacccg ccgccaggcc gcgcgccagg
    10501 cgtaggcccc tgcacatgtc agtgcgaatt cgacgcgcca catttcgggg cagaccagaa
    10561 accggccgac aagtgtttcg cccttttccg gatgccacag ccgcgggtcg agtgtgaaaa
    10621 gccgccgata gccgctcgag tatgcgcgct caaccgtggt ggcgtcgtaa tcgccgtaag
    10681 gaaaggccaa gtccacgatt tcgcaatcga gaagctcttc cagcgcctct ttcgagtcgg
    10741 acaactcgcg catcatttcc gctctcttgg cctgactcag cctgacgtgc gagaacgtat
    10801 gtgaaccaat cgagcacagg ccgcgtcgcg cggccgcggc cagctccggt ccactcgcga
    10861 cccgctcctc gcgatccggg tggccgaccg gcatgcccca ggcgggaacc atgccaaagc
    10921 tgcccggaac tacgaacagc gtcgctggga caccgagcgc ctgcatggcc ggcagggcat
    10981 ttttcagcac gttttcgaag ccgtcgtcga aggtcacgca gatgtacggc gaccccccgg
    11041 aggctctccg tccggaggcg accgcggacg ggctcacggc gcgccgcgca gtgtggcgca
    11101 tctgccaggt gaaacgctgc agttgatcag tgcgcacgga gtggtaacat aggaccacga
    11161 ttccccgcgg tcttccagcc aggcgcgtaa gaagccagta tatagcagag attatcacgt
    11221 aagcaatgcg caccatctgg ccgcgccgcg cgtacgggct tcggtagcgt ccaagttgta
    11281 tttcggcctt agacacgata gcgcgtccac aacgtccata ggccgcgccg cggcagcggc
    11341 aagcacggcg cgatgcgccc aaaccaaatc tggtagtaca acaggtgcag cgcgtcggat
    11401 actacagata gcaagcgggt cttcacgcgt ccgatcttgc cgcgcgccac cactatcgac
    11461 tgtagagggt attccagcgc ccccatggcc gttttgtagt cgtagcggcc cgggccgtcc
    11521 tccgcgcgcc gcactccggc ggcaatcgcg gcctcaaccg cccgtacctg gaccagtata
    11581 cccaggtgtg cgcgattcca ctgtggccgc acgtcgcgcg ctgacaagcg ccagtgctca
    11641 tgcgtcccaa agcgcagaaa gtagttgctg gacacgagtt ccccgtccgc ccgcaggttg
    11701 cagaacccgg cccggttttc ggcagccagc gtacgcacga ggtcgcggtt gaactccagc
    11761 gcgcccggcc agtcgtgaaa gtggcccagt ttccgagccc ggcgccactg ggaggtgtgc
    11821 agggacacga aggcctcgta agcgggcacc gcggcgtcac cgctgacgct ctccacggcc
    11881 acctggtgct tggtggtgag ccggttccag ccacgttcgt attcccggcg ctgcgttttc
    11941 tgcaatccgc gcaggtaagt gtcgctatcg gccggcaagg tgtacacgtt atgcacggtg
    12001 gtcgcctggt cgcgtaccac gtacgtcccg ggcgacccct ggcacgcggc ccgcacggtt
    12061 cgcgcgtgcg gccgttcgcc ggaaagcgga ccgaacgata ctgcgtcaca cttgtgcacg
    12121 tagagaagtt gccgcagcac atcagcgtac acttggcgcg cctgatccgg ctcgactggg
    12181 gggtttagca ctgccagagt ggaatcagag ccgatgaatt tggccaggcg cacagtgacc
    12241 ggtccgaggc gcaggcggtc gataaacatg ggcaggatgg cgactaattc gccggcggcg
    12301 cgaaatacga acacgcacag c
//
LOCUS       DAOSYS010000094        12020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4499_length_12020_cov_5.479649, whole genome shotgun sequence.
ACCESSION   DAOSYS010000094 DAOSYS010000000
VERSION     DAOSYS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12020
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_12020_cov_5.479649"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PLU99_07510"
     CDS             complement(<1..552)
                     /locus_tag="PLU99_07510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO16940.1"
                     /translation="MPYTFRHGDRPLEGVTVQRAVGRGGFGEVYYALTDSGKQVALKY
                     LRENPEIELRGIAQVMNLKSPHLITIYDVRRNTAGEPFVLMEYVSGPSLRELLTDAPH
                     GLGPQKAAYFIDGIAKGLAYLHDRGIVHRDLKPANIFYDDGYVKIGDYGLSKHISVSR
                     HSGQTVSVGTVHYMAPEIANGRYG"
     gene            complement(580..2226)
                     /locus_tag="PLU99_07515"
     CDS             complement(580..2226)
                     /locus_tag="PLU99_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOO16941.1"
                     /translation="MFSRWLNVRVRAAERALEEGRLDEAFRLAVEPEVRGDARAGRLL
                     QGLSRRLLARARLAREGGWYERALGDLDRLRVIGLSSAEAEELRAQVIREMDRKHQAA
                     AQRRAAVEHDAAQRRAAVEKAAADLKAGRLESGRLAVECVTDERGREELREQLDVRLQ
                     RSGQLLRQAGEALERGETLIALRFWQEARDRHGRTAESDEFAVRLSGALRRACDEWIA
                     GGRLEPLLAAAGGLAALRAVEPGSDELERLAGLCGRAAGQLGSRDYQGLRETLLRLRA
                     ARQGVGWIEETLAALTKIAEGQEALLSSPLGLLASMRVEKSEPEAPAMARAAGRTDPT
                     AALNRLETDLTDTGALLMLVDGAGSFLVLPHDCVRIGRAGRDASVDVPIPADVQSHHA
                     DITRDGEDYFLTAYGPVQVNQRDVRRTLLRNGDRIVLGPKAKMVFHKPSAKSDSAVLR
                     LSHRCRLAQDVSGIVLFHQTCLIGPQSSCHVRTVEGQTQVVLFERGGRLYGREAASGG
                     GKLGDPRELAAGATYDFGEVRIALKRYDDGQSCGRRGDLA"
     gene            2494..3684
                     /locus_tag="PLU99_07520"
     CDS             2494..3684
                     /locus_tag="PLU99_07520"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO16942.1"
                     /translation="MIEANETNTPVPPLNLAGNFAALSGELMAELAEVAASGYYVLGP
                     KVAAFEAALAKYCGTRYAIGLSSGTDALLAALLALEVGPGDEVVVPTFTFFATAGVVA
                     RLGARPVFCDIEPEAFNIDVRALERALTPRTKAIIPVHLYGQLADMEAVLEIANRRNI
                     PVIEDAAQAIGAHCDINSPEASAGPAPQSGRKMAGSFACMGALSFYPTKNLGALGDAG
                     AVLTDDSRLDEILRKLRVHGSGHTYYHDRIGGNFRIDAIQAALLTIKLKHLETWTEQR
                     IARAARYTQLLEAAGLVPEHVRPPTVLRGRHVYHQYVIRAQRRDELAEFLKQRKIGCG
                     VYYPLPLHLQTCFKHLGYKPGDFPVAEAAAREVLALPIYPELTEEQQRAVVEGIAKFY
                     GRGA"
     gene            complement(3889..4284)
                     /locus_tag="PLU99_07525"
     CDS             complement(3889..4284)
                     /locus_tag="PLU99_07525"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOO16943.1"
                     /translation="MESAFVNIDSNQEPERGRSWRDALLYPYAYLLYILLSSLDIIIT
                     WTILRAGGQELNVIADWVIRNYDRVGVVVYKFALLVIVVLICETVGRRNPTMGQRLAY
                     WAVALTAFPVVVGLVHLLSAMLAYRAPAL"
     gene            4578..5564
                     /locus_tag="PLU99_07530"
     CDS             4578..5564
                     /locus_tag="PLU99_07530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOO16944.1"
                     /translation="MEFRELGRNGPEVSAIGLGCMAMSEFYGPHDDEQSVNTIRRALD
                     LGVNFLDTADVYGRGHNEELVGRAIRGRRNRVILASKFGIVRSADPEARGVSGHPKYV
                     KACCDASLKRLGVDTIDVYYQHRVDPQVPIEDTVGAMAELVQAGKVRFLGLSEAGAET
                     LRRAAAVHPITALQSEYSLWTRDVEQEILKTCRELGVGFVAYSPLGRGFLAGQVRRLE
                     DLAENDWRRFNPRFARENFSRNLALVQQLEEFAAGKGCTSAQVALAWLLARGKDIVPI
                     PGTRRIQRLEENIRALEVQLTPGDMQRLDELFQPGAAAGTRYPEAMMALLNK"
     gene            complement(5655..6536)
                     /locus_tag="PLU99_07535"
     CDS             complement(5655..6536)
                     /locus_tag="PLU99_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HOO16945.1"
                     /translation="MKTARRGVLYATAGFVAVGPLLVWFLAHAPQRAAGRDAVPAAML
                     GPIQKEGISGLCASSDSSLWLLAQTDPMALARMGHERYQNEINDYHCVLVKQERLPGG
                     LSDVQEIEVRFRKEPRSVYMIWRENASDAKRALFMPEDPRFTDDRGNLMARVEPAGSV
                     VRLFVKDLYIPIHSERARNASRRTIDECGFGAMFEMLSQINNLARERGVLDLKFAGAG
                     EVDDRPTFVLVRKLPYTGEKGVFPDARMVLHIDQQWLLPVAVESFADEGGAQLLGRYV
                     FTDIHLNPGLTEKDFTF"
     gene            complement(6715..7578)
                     /locus_tag="PLU99_07540"
     CDS             complement(6715..7578)
                     /locus_tag="PLU99_07540"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO16946.1"
                     /translation="MRRPSITPQIQSWKMSDGYELRGRVWPPRNPAAGVILYFHGIQS
                     HGGWFEWSASLLGESGAGVLLVDRRGSGLNQVARGDAPSVERWLQDIDEYAAWAGREF
                     GATRFPLVGVSWGGQLAAAWAARRPNIAGAVLLIAPGFFPAVDLSVLAKLGVGLALLA
                     GGGRSFEIPLDDPALFTDNPSGREYIQSDPIRLRRVTARFMWHSRQLNKLVRGLKAGA
                     LGSQVTLLLAGRERIIRNTITELWVRSICRTARVVRFPDAAHTLEFEPNLSEFSAALR
                     NWARHPARDIG"
     gene            complement(7588..8046)
                     /locus_tag="PLU99_07545"
     CDS             complement(7588..8046)
                     /locus_tag="PLU99_07545"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HOO16947.1"
                     /translation="MKPFETAEGAEYHGCRQLNIFLENRLGQLLRITRLLEAEPIRIL
                     GLSVEGKVDCAIVRMLVDDPDAADRMCSEAGFACTESEVLVVELPAGKRAIMTVCAAL
                     ISAEVNINYVYTAWAGDEQKPALVIQVDNLPQAMRVLAHKGFSMLAQDEL"
     gene            8163..10370
                     /locus_tag="PLU99_07550"
     CDS             8163..10370
                     /locus_tag="PLU99_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO16948.1"
                     /translation="MRLALAEILFAQPALLGGIALAAVPIVIHLLLRPRPRRTRFPAI
                     VLLRRVVLTGQRSSRFQNLMLMLVRAAFLALLALLLAAPVRTDGAVDFVGPDPLLCAV
                     VLDESASMNYRVDGATTRLDLSRAAATDFVHQSATWPEGSALVVLTSAMPEETLPEPG
                     NDRPALLARLEGAGLRDSAKPLGTAISAAAQVLRTAQQPVRRLVVFSDGAAAAWRDVN
                     PAALAGIENLAVRVVTSGGQPQSNLALTSAEGPQRLQPATARIPVRAAITAEGVDTEC
                     WLTVHAAGQKLHRVGPVQVHAGSLKHVEFDLPPLPPGPHLLTFELEPADRLDFDQQRF
                     VAVQTAEPPVVWLIGAGDTDNDLSAVIYRNLLAPEVLSAEQQRVKLLNLSGEQLAAGS
                     GRSPSAAGEAADSDSQPVLAVVLSNADLSREAQAVLLRQLERGMTVVLAPASTSGECD
                     WPGLRQLLSDDLPADEELSEPTSFVWKPESEFAGHSELIELSRCGVLRRLRLGELQKE
                     VEVHAQYADERPAVVSRRRGSGRLFLLTTAPDPQWSDLGIRAAGLLSWLHLLVDEALG
                     PPDAVADFALGEPARNSFPTLPAGGLIRVAPQGARNAKPEWIRLKNQAPEQFWPTDKA
                     GAYALEPAGAGHLAAAYTVNWPIEESVLAPITAAKLEQALGVKDVRLESTDSASAERL
                     GLWARLFNIRDLRKVLAVLILILFVGELLLFRRLAHRRPTANATFADKADAAC"
     assembly_gap    10399..10498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11325..>12020)
                     /locus_tag="PLU99_07555"
     CDS             complement(11325..>12020)
                     /locus_tag="PLU99_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16949.1"
                     /translation="AGERREPRCPAGAVCPQPGMTDSDALPPNARPGECYTKVYVPPE
                     FATVTERIKVRDAYDELEVVPARYEWVEERVCVKEASEELRVVPAQFAMRPVTFEAQA
                     GGTEWQVNANPTCVAYPGQPAQDVFCLVSHPPLLQTIPTQTLVKPAHVEKVRIPAQFQ
                     TIRRQKLVSPAMVRRVTIPAEYRDIQKNIKVCDGKMVWKRIECNAGQGVVSDASDTTP
                     QTPPATALAADRN"
BASE COUNT         2165 a         4036 c         3643 g         2076 t
ORIGIN      
        1 accgtaccgt ccgttggcga tttccggggc catgtagtgg accgtgccca cactcacggt
       61 ctgcccgctg tgccggctta cggagatatg cttgctcagg ccgtagtccc caatctttac
      121 gtagccgtcg tcataaaaga tgttggccgg cttcaagtcg cgatgcacga tgccgcgatc
      181 gtggagatac gccagcccct tcgcgattcc gtcgatgaaa tacgccgcct tttgcgggcc
      241 cagcccgtgc ggcgcatccg tcaacaactc gcgcagcgat ggcccgctca catattccat
      301 cagcacgaac ggctcaccgg ccgtgttgcg ccgcacgtca taaattgtga tcaggtgcgg
      361 gctcttgagg ttcatcactt gcgcgatgcc gcgcagctca atctccgggt tctcgcggag
      421 gtacttcagc gccacctgct tgccggaatc ggtcagggcg tagtacacct cgccgaagcc
      481 gccgcggccc acggctcgtt gcaccgtaac gccttccagc gggcgatcgc catgtcgaaa
      541 cgtatatggc acctcggttc ctccgggcct gctccagccc tacgccaaat cgccgcggcg
      601 accgcagctc tggccgtcat cgtagcgctt cagtgctatg cgcacttcac caaaatcgta
      661 agtcgcgccc gccgccagct cgcgcgggtc gcccagcttg ccgccccccg aggccgcctc
      721 gcgcccatac aaccgcccgc cgcgctcgaa cagcaccact tgcgtctgcc cctcgacggt
      781 ccgcacgtgg cagctcgatt gcgggccgat caggcacgtc tggtggaaca acacgattcc
      841 actcacgtcc tgcgccaggc ggcagcgatg cgatagccgc aacaccgccg aatcgctctt
      901 ggcactgggc ttatgaaaga ccatcttcgc tttgggaccg agcacgatgc ggtcaccatt
      961 ccgcagcagc gtccgccgta cgtcgcgctg attcacctgc accggcccgt atgccgtcag
     1021 gaaatagtcc tcgccgtcgc gcgtgatgtc cgcatggtgc gactgcacgt cggccggaat
     1081 cggcacgtcc acgctcgcat cccgccccgc tcgcccgatg cgcacgcagt catgcggcaa
     1141 aaccaggaag ctcccggccc cgtctacgag catcaagagc gcgcctgtgt ctgtgagatc
     1201 ggtttccagc cggtttaatg ccgctgtggg atcggttctg cctgcggctc tggccatcgc
     1261 cggcgcttcg ggctctgatt tctcaacccg catcgacgcc agcaatccca acggcgatga
     1321 tagcaacgcc tcctgcccct ccgcgatttt cgtgagcgcg gccagcgttt cctcgatcca
     1381 acccaccccc tgccgcgccg cgcgcagccg cagcaacgtc tcgcgcaaac cctggtaatc
     1441 tcgactccca agctgccctg ccgctcgtcc gcacaacccg gccaaccgct cgagctcatc
     1501 gctacccggc tcgacggcac gcagcgccgc caacccgccg gccgccgcaa gcagcggctc
     1561 caaccgcccg cccgcaatcc actcatcaca cgcacgccgt aacgccccgc tcaagcgcac
     1621 ggcgaactca tcgctctccg ctgtccgccc atgacggtcg cgcgcctcct gccagaatcg
     1681 caacgcaatc agcgtctcac cgcgctccag cgcctcgccc gcctgccgca gcaactgccc
     1741 gctgcgctgt agccgaacat caagctgttc gcgcagctct tcccgccccc gctcgtccgt
     1801 gacacactcc accgccaacc gccccgattc cagacgaccg gccttcagat cggcggccgc
     1861 cttctccacg gccgcccggc gctgcgccgc atcatgttca accgccgcgc gccgctgcgc
     1921 cgcggcctga tgcttgcgat ccatctcgcg gataacctgc gcccgcaact cctccgcctc
     1981 cgctgaggaa aggccgataa ctcgcagccg gtcgagatcg ccaagcgccc gttcatacca
     2041 gcccccctca cgagccagcc gtgcccgcgc tagcagccgc ctactcaacc cttgcagtaa
     2101 ccgccccgcc cgggcgtcgc cgcgcacctc cggctcaacg gccagcctga acgcctcgtc
     2161 cagccggcct tcctccaggg cccgctcggc ggctcgaact ctcacattta gccagcggct
     2221 gaacatccgt aatcctcggc cactgccacg gccggcagcc cgcattttat cgccagctca
     2281 ggccgcggcg cggcacgggc gccgctcgcc ttcgagcagc ggcctgcaac gtgactgaat
     2341 tccaggaacg gcccgcgata tttccgcaag acgcgccggg ccggcaaacc cgccggattt
     2401 tgccaaaact gccgtcggca gcggcgagca tgcggcgcct gcttcaaatc ctgagtaatc
     2461 gcggttaccg tgatctgccg caggaggatg tgcatgattg aagcgaatga aaccaacacg
     2521 cccgttccgc cgctgaacct ggccggcaac ttcgccgcgc tttccggaga gctcatggcg
     2581 gagctcgccg aggtggcggc ttcgggctat tacgtactcg ggccgaaggt ggccgcattt
     2641 gaagcagctt tggcgaagta ttgcggcacg cgttatgcaa ttgggctgtc gtcggggaca
     2701 gacgcgctgc ttgcggcgct gctggcgctg gaagtcggcc cgggcgacga agtcgtcgtg
     2761 ccgacgttca cgtttttcgc gacggccggc gtcgtggcgc gcctcggcgc ccgcccggtc
     2821 ttctgcgata ttgagcctga agcgttcaat atcgacgtgc gagcgctcga gcgcgccctc
     2881 acgccgcgca ccaaggccat cattccagtg catctctatg gccagctcgc ggacatggag
     2941 gcggtcctgg aaattgcgaa ccggcggaac attccggtaa ttgaagacgc cgcgcaggcc
     3001 atcggggcac actgcgatat caatagcccg gaggccagtg cggggccggc tccgcagtcc
     3061 ggccggaaaa tggccggcag ctttgcctgc atgggcgcac tcagtttcta cccgaccaag
     3121 aatttgggcg cattaggcga cgccggcgcg gtcctgaccg atgactccag actggacgaa
     3181 atcctgcgca aactgcgcgt tcacggcagc ggccatacat actatcacga ccgcatcggc
     3241 ggcaacttcc gcattgatgc aatccaggcc gccttgttga caatcaagct caagcacctc
     3301 gaaacctgga cggagcaacg cattgcccgc gcggcgcgct atacccagct attggaagcc
     3361 gccggcctgg tacccgaaca cgtgcgccca ccaacagttc tccgcggccg gcacgtctat
     3421 caccagtacg tgatccgcgc ccagcggcgc gacgaattgg cggaattcct gaaacagcgc
     3481 aagatcggct gcggcgtgta ctatcctctg ccactgcact tgcagacctg cttcaagcac
     3541 ctcggctata agccgggcga cttcccagtg gccgaagccg cggcccgcga agtactcgcg
     3601 ctgcccatct atcccgaact gacggaagag cagcagagag cggtcgtgga aggaatcgcg
     3661 aagttctacg gccgaggcgc ataggcgcat aggcgcatag gcgcgtaggc gcataggtgc
     3721 atgggcggat gggtggatga gtggatgagt gggtgccatg cccaagctgt aagcgcgggc
     3781 atgccccttt aacggcgtca agtgcggatg gttgacgacg tcaggccgcg gtttctgcac
     3841 tgctgaagcg cctgcttcag cagtggcaca cacagcacac acagcgcact aaagagccgg
     3901 cgctcggtat gcgagcattg ccgatagcaa gtggaccagg ccgaccacta ccggaaaggc
     3961 cgtcaacgcg accgcccagt acgccagccg ctggcccata gttggatttc gacggccgac
     4021 ggtttcgcag atgagaacga cgatgaccag cagcgcgaat ttgtacacca ccacgcccac
     4081 gcggtcatag ttgcgaatca cccagtccgc gatgacgttc aactcctgcc cgcccgcccg
     4141 cagaatcgtc caggtgatga tgatgtcgag cgacgataac aaaatgtaca gcaggtacgc
     4201 gtaggggtac aacagcgcat cgcgccacga ccggccgcgc tcaggttctt gattcgagtc
     4261 gatgttgacg aaggcgctct ccacgcgaca cacacctgca accctccata tccggccccg
     4321 gttggggcgg gataggacgg ccgtcttcct caaccagaaa tcctgtcctg cccgccgaaa
     4381 aagtcaactt tcacttccgt tggcccgttg gcaagctcgc ccgccgttgg acgtcaggac
     4441 ggcggctgat tatcataata gcatcgcagc caacgttgcg aacggcacat gaaaagtgct
     4501 tccatttgag cgccgtgcct tgcggcgggg tgaggccggg ggccacggcc ggttcatcag
     4561 gcagaaggag cacgcagatg gaattccgcg aattggggcg aaatgggccg gaggtttccg
     4621 ccatcggcct gggttgcatg gccatgtcgg agttctacgg cccgcacgac gacgagcagt
     4681 cggtcaacac catccggcgc gccttggacc tgggggtgaa tttcctggac accgccgacg
     4741 tgtacgggcg cgggcacaat gaggagttgg tgggccgggc gattcgcggg cggcgcaacc
     4801 gcgtgatact ggcgagcaag ttcggcattg tccgtagcgc agacccggag gcgcgcggcg
     4861 tcagcggcca tcccaagtac gtgaaggctt gctgcgacgc gtcgctgaag cgattggggg
     4921 tggacacgat cgacgtttac taccagcacc gcgtcgatcc gcaggtgccg attgaggaca
     4981 ccgtcggggc catggccgag ttggtgcagg ccggcaaagt acgattcctg gggctgtcgg
     5041 aagcgggggc ggagacgttg cggcgtgccg cggcggttca tccgatcacg gccttgcagt
     5101 cggagtattc gctgtggacg cgcgacgtgg agcaggaaat cctgaagacc tgtcgcgaac
     5161 ttggcgtggg ttttgtcgct tattcaccgc tggggcgcgg ctttctggcc gggcaggtgc
     5221 gccggcttga ggatctcgcg gagaatgatt ggcggcgttt caacccgcgc ttcgcgcgtg
     5281 agaacttcag ccgcaatctg gcgctggtac agcagttgga ggagttcgcc gcggggaagg
     5341 gctgcacatc ggcgcaggtg gcgctggcct ggctgctggc gcgcggcaag gacatcgttc
     5401 cgattcccgg cacacgccgc attcagcgcc tggaagagaa cattcgcgcg ctggaggtcc
     5461 aacttactcc cggcgacatg cagcgtctgg atgagctgtt ccagccgggc gcggccgcgg
     5521 gcacgcgcta tccggaggca atgatggcgc tgctcaacaa gtaagcgccg ggcctcgtgt
     5581 gccgatacaa aaaaacgcgg ccccgccgag cgccgctcga ccgggccgcg ccttttagct
     5641 cacgtatata taacctagaa cgtgaagtcc ttctcggtca gaccggggtt caagtggatg
     5701 tcggtaaaga cgtaccggcc gagcagttgc gcgccgccct cgtcggcaaa agattcaacc
     5761 gccacgggca gcagccactg ctgatcaatg tgcagaacca tgcgcgcgtc ggggaagaca
     5821 cccttctcgc ccgtgtacgg cagcttgcgc accaacacga acgtgggacg gtcgtccacc
     5881 tcgccggcgc cggcgaactt caggtccagc acgccccgct cgcgcgccag gttgtttatc
     5941 tgcgaaagca tttcgaacat cgccccgaag ccgcattcgt cgatcgtgcg ccggctggcg
     6001 ttgcgcgccc gctcactgtg tatcggtatg taaaggtctt tcacaaacag ccgcaccacc
     6061 gagccggcgg gttcgacgcg tgccatgagg ttgccgcggt cgtcggtgaa gcgtgggtcc
     6121 tcgggcatga acagggcccg cttggcatcg ctcgcgttct cgcgccagat catgtaaacg
     6181 gagcgcggtt ctttgcggaa gcgcacctcg atttcttgga cgtcgctgag gccccccggc
     6241 aaccgctctt gcttcaccag cacgcagtga tagtcattga tctcgttctg atagcgctcg
     6301 tgacccattc gggccaaggc catgggatca gtctgcgcca gcaaccaaag cgaactgtcg
     6361 ctgctcgcgc acagccccga aatcccctct ttctgaatgg gccccaacat tgccgccggc
     6421 acggcgtctc ggcccgccgc gcgctgcggt gcatgtgcca aaaaccaaac cagcagcggc
     6481 ccaacggcca cgaatccggc tgtcgcgtac agcaccccgc ggcgggcagt cttcattggt
     6541 agctccatac tgcaagcggc tcagcccatt ctgccgcaaa ggggcgtcgt cgttgtcgcg
     6601 cccggatttc aggcttactt ccgagcctcc gggggtgctc gccgcgccga ggtcccgtca
     6661 attatacggg gttgtacctg ccggacaagc cgcggttgca tatgaagatg caaattaacc
     6721 gatatcacgt gccggatgcc gcgcccaatt ccgcagtgcg gccgagaatt ccgacagatt
     6781 tggttcgaat tcgagcgtat gggcggcgtc gggaaagcgc accacccgcg ccgtcctgca
     6841 gatgctgcgc acccagagtt cggtaatcgt atttcgaatg atgcgctcgc gcccggccag
     6901 aagcagcgtt acttgagatc ccagcgctcc cgctttcaac cctcgcacga gcttgttgag
     6961 ctgccgcgag tgccacataa aacgggccgt gacgcgcctc aggcgaatcg gatcggattg
     7021 gatgtactct cggccggacg ggttgtccgt gaacagagcc gggtcatcca ggggaatttc
     7081 gaacgaccgc ccgccgcccg ccagcagcgc caaccccacc ccgagcttgg ctaagaccga
     7141 caggtcgacg gccggaaaaa aaccaggtgc gatcagcagg acggccccgg cgatgttcgg
     7201 ccggcgcgcc gcccacgccg ccgccagttg tccgccccag ctcacaccaa cgagcggaaa
     7261 gcgcgtcgcg ccgaactcac ggcctgccca ggcggcgtat tcatcgatgt cttgcagcca
     7321 gcgctcgacc gagggcgcgt caccccgggc aacctggttc agcccgctgc cacgccgatc
     7381 gaccagcagc acgcccgccc cgctttcgcc caacaacgaa gcggaccact cgaaccagcc
     7441 gccgtgcgac tggatgccgt gaaaataaag aataacgccg gccgccggat tgcgcggcgg
     7501 ccacacccgg ccccgcagct cgtacccatc gctcattttc caggactgaa tttgaggcgt
     7561 aatcgacggg cgccgcatgg ctttggctca gagttcgtct tgcgccagca tggagaaccc
     7621 cttgtgagcc agcacccgca tggcctgcgg cagattatcg acctgaatca ccagcgcggg
     7681 tttttgctca tcccccgccc aagcggtgta aacgtagttg atgttcactt cggcggaaat
     7741 cagcgccgcg cagaccgtca tgatggcgcg cttgccggcc ggcagttcca ccaccagcac
     7801 ctcgctttcg gtgcaggcga agccggcctc cgaacacatg cggtcggccg cgtccgggtc
     7861 gtccaccagc atgcgaacga tagcacaatc caccttcccc tcgaccgaaa gtccgagaat
     7921 acggatcggc tccgcctcca acagccgcgt gatgcgcaac aactgcccca gccggttttc
     7981 gaggaaaatg ttgagctgcc ggcacccgtg gtactcagcc ccctcggccg tttcaaatgg
     8041 tttcacgccg ctcatacggt ccccgacagt tcagaaaacc cggtactgac cacgcgcgca
     8101 aatcctaggg tagctttgcc aaacgcgtct gtacaggcca aaatcataaa tcggattgcc
     8161 tgatgcggct cgcccttgct gagattctat tcgcccaacc ggccctgctt ggcggaattg
     8221 cgctggcggc tgtgccaatc gtaatccacc tgctcttgcg gccgcgcccg cgccgcacac
     8281 gctttcccgc cattgtgctg ctgcgccggg tggtcctcac cgggcagcgc tccagccgat
     8341 tccaaaacct gatgctgatg ctggtgcgcg cggcgttcct ggctctgctg gcgctgctgc
     8401 tggccgctcc ggtgcgcact gacggcgcgg tcgacttcgt cggtcctgat ccgctgctat
     8461 gcgcggtcgt cctggacgag tcagccagta tgaactaccg cgtggacggc gccactacac
     8521 gcctcgacct ttcgcgcgcc gccgcgacgg acttcgtgca tcagtcggct acctggccgg
     8581 aaggttcagc actcgtcgtg ctcaccagcg ccatgcccga agaaaccctg cccgaaccgg
     8641 gcaacgatcg ccccgcgctg ctggcacgcc tcgaaggcgc cggcctacgc gacagcgcca
     8701 aaccactcgg caccgccatc tccgccgccg cccaagtgct tagaacggca caacagcccg
     8761 tacggcgcct cgtggtcttc tcggatggtg cggccgcggc gtggcgcgac gtcaatccag
     8821 cagccttggc cggcattgag aatttagccg tacgcgtcgt caccagcggc gggcagcccc
     8881 agagcaatct ggcgctcacg tccgccgagg gaccgcagcg gctgcaaccc gccacggcca
     8941 gaatccccgt acgggcggcg atcacggcgg agggcgtgga taccgaatgt tggctcacgg
     9001 tgcacgccgc cgggcagaag ctgcatcgag tcgggccggt acaggtccac gccggctcgc
     9061 tcaagcacgt ggaattcgat ctgccgcctc tgccgcccgg cccgcacttg ctcacgttcg
     9121 agctggagcc ggccgaccgc ctagacttcg accagcagcg gttcgtagcc gttcagacgg
     9181 ctgagccgcc ggtcgtgtgg ttgatcgggg ccggtgacac ggataatgac ctatcggcgg
     9241 tgatctaccg caatctgttg gcgcccgaag tgctcagcgc cgagcagcag cgcgtcaagt
     9301 tgctaaattt gtctggggag caactggcgg ccggcagcgg acgatcgccg agcgcggcgg
     9361 gcgaggcagc ggatagcgat tcgcagccgg tcctggccgt cgtgctctcc aatgcggatc
     9421 tgtcgcgtga agctcaagcg gttctgctgc gacagctcga gcgtggaatg accgttgtgc
     9481 tcgcgccggc ctcgactagc ggcgagtgcg actggcccgg gctgcgccaa ctgctgagcg
     9541 atgatttgcc ggccgatgag gaactttccg agcccacctc attcgtctgg aagccggagt
     9601 cagagtttgc cggccattcc gagttgattg agctgtcacg ttgtggtgtt ttacggcgtc
     9661 tacggctggg ggaattgcag aaagaggtcg aggttcacgc acagtatgcg gacgagcggc
     9721 cggccgtcgt ttctcgccgg cgcggctcgg gacgcctgtt tctacttacc accgcgccgg
     9781 atccgcagtg gagcgacctg gggattcggg cggccgggct gttaagctgg ctgcacctgc
     9841 tggttgacga ggcgctcggt ccgccagatg ccgtcgccga cttcgcgctg ggcgagccgg
     9901 cccgcaactc ctttcctacg ctgccggccg gcggtttgat tcgcgtcgca ccgcagggcg
     9961 cgagaaacgc gaagcccgaa tggatcaggt taaagaacca ggccccggaa cagttctggc
    10021 cgaccgacaa ggccggtgca tacgcgctcg agccggccgg cgccggccac ctggcggcgg
    10081 cctacaccgt caactggccg atcgaagaga gtgtcctggc gccgatcacg gcggcgaaac
    10141 tcgagcaggc attgggagtg aaagacgtgc ggctcgagtc gaccgacagc gcttcggcag
    10201 agcggctcgg actgtgggcg cgtctgttca acatccgcga cctgcgcaaa gtcctggcag
    10261 tattgatcct aatcctattt gtcggcgagt tgctgctatt cagacgcctg gcccacagac
    10321 gcccaaccgc taacgcaaca ttcgcagata aggctgacgc cgcgtgttag tagcagtgcc
    10381 gcggcgtgcc gcccagaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    10501 agataaggct gacgccgcgt gttagtagca gtgccgcggc gtgccgccca gaacatcgaa
    10561 gggatcgagg gatcaaggga ttgagggatc aagaggtata aagagcagag ccggggcgtg
    10621 tcgccccgga atgccgccca aaatcgccca tccgcgcagt tgcccgtctc gcatccggcg
    10681 tgccgtcccg gaatgctaca gataatgtta cattcgcgca gttgcccgcc gcgtctgggg
    10741 ctcgccccgg actgccgcac agattcccca tccgggcaat aatcactgcg actacaccct
    10801 ccgcccggaa tggcgcaaga atctccatcc gcccggtgcg cgctggcccg gccgtgtgcc
    10861 actgctccag gcagaacgac gacgggcatt tagaccacat cgatgtaggg atgcttcagg
    10921 agccctttca cgattagtaa cagagccggc tcgtgtcgag ccggaatatg accagagtac
    10981 cgccaatcgc tcaggtcgca cgcccggcac gcagcgcagt ggcgcgcagc gcgcactgcc
    11041 gtgggggggt tggaaacgaa ttccgtcagc cccgtgggga cggctgaaaa tagcccagca
    11101 cgcagtgctg gggaaagttt ggagacgcaa tcctttagtc ccgtagggac gatcgaaggc
    11161 tccgaacgac ggaaagctca atcgtcccta tgggactgcg gtcgccaagg ccgcccacgc
    11221 cgctctaaac gctccatgcg ggcactgccc ggcgtcgcgc cgcggtgtgc agaaagaacc
    11281 gcgctaaagg ccggcgctaa cggcacccga cgactgacga ctaactaatt tcggtctgcg
    11341 gccagcgcgg tcgcgggcgg ggtctgcggt gtggtgtcgg acgcgtcgct caccacaccc
    11401 tggcccgcgt tgcattcaat tcgcttccac accatctttc cgtcgcagac cttgatgttc
    11461 ttttgaatgt cccggtactc cgccggaata gtcacacgcc gcaccatggc cggtgagacc
    11521 agcttctgcc gccggatcgt ctgaaactga gccgggatgc ggaccttctc cacgtgggcc
    11581 ggcttcacca aagtttgcgt gggaatcgtc tgcagaagcg gcggatggct caccaggcag
    11641 aacacgtcct gcgcgggctg accggggtag gcgacacaag ttgggttggc gttaacttgc
    11701 cactccgtcc cccccgcttg cgcctcaaat gtcacggggc gcatggcgaa ttgcgccggc
    11761 actactctga gttcctccga agcctctttg acacacacgc gctcctcgac ccactcataa
    11821 cgggccggga ctacttcgag ttcatcgtaa gcgtcgcgga ccttgatgcg ttccgttacg
    11881 gtcgcgaatt cgggcggcac gtacaccttg gtgtagcact cgccgggcct ggcgttcggg
    11941 ggcaaggcgt cggaatccgt cataccgggt tgcggacaaa ccgcgccagc cgggcagcgt
    12001 ggttctcggc gttcgccggc
//
LOCUS       DAOSYS010000095        11946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4536_length_11946_cov_5.633084, whole genome shotgun sequence.
ACCESSION   DAOSYS010000095 DAOSYS010000000
VERSION     DAOSYS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11946
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4536_length_11946_cov_5.633084"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLU99_07560"
     CDS             <1..638
                     /locus_tag="PLU99_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16950.1"
                     /translation="DLDFYRYELSGTDPACTILQAKVRGEFEPDIWLMVGECFTDSWC
                     GVEETYVMVYSRAAGGVCDYAIAAAPLPSDPPQYVYIMVSCFGSVGDVPCNTPYTLEI
                     EKVAAGCCRIGEASGITSEAYCLALGGEWTAGDCCGEQECVLADSNCDGAVNVFDIDA
                     FVLALTNPAQWLATYTCDICNNDCNCDGEVNVFDIDPFVQCLTSGGACTCP"
     gene            complement(965..1666)
                     /locus_tag="PLU99_07565"
     CDS             complement(965..1666)
                     /locus_tag="PLU99_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO16951.1"
                     /translation="MPYRNMSIDELARHIGMDARIVRKWAEKGRLPGSMVQGQWRFNR
                     AQMLDWLQQEIHNLDPKHIANLERAMSDGNDEEVLGALLPPEAIDMNLPARSRTSVLK
                     ELISLAERTGLVYDPIGLLTAVQEREELYPTALPHGVALPHPRRPLPYATAEPLVCLA
                     RVPAGIPFGAPDGQLTHIFFFVCSHEERQHLCVLARLAMMLSTDLPARLRDIDDPAEA
                     LALILESEQRVIARR"
     gene            complement(1826..2575)
                     /locus_tag="PLU99_07570"
     CDS             complement(1826..2575)
                     /locus_tag="PLU99_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO16952.1"
                     /translation="MNQLMYNLHNHTPFSDGAYTIDEIIQAHLDDPELNMGGIGICDH
                     LFCTPSSREVNNEREFHRVFDRETQEYVNAVHEARRKWADRVPVYCGAEINWQMNKGL
                     LDTIKTMLTGIDYVLFEYVDWAGLTTLANQARRWPCPVGLAHAAVAQQFPSTSMDQVV
                     RTLANARIFYELNSKFIPLEEHDRWFNILPNHRVLISIGTDTHDDLSVLKTIPRLHDY
                     ARQHGLAEKIFVPKYAGADAGAPTPAGVSKS"
     gene            2945..3160
                     /locus_tag="PLU99_07575"
     CDS             2945..3160
                     /locus_tag="PLU99_07575"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16953.1"
                     /translation="MLQGLAQGQWRQACLHNPLIFTVVLIAPLVFLARGLWRRSSVLR
                     QRSMTIVYVLLATVFVNWVYLLLREPP"
     gene            complement(3229..3978)
                     /gene="sdhB"
                     /locus_tag="PLU99_07580"
     CDS             complement(3229..3978)
                     /gene="sdhB"
                     /locus_tag="PLU99_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOO16954.1"
                     /translation="MANQIHFRIKRQAGPDAQPYWEDFIMPDAGGHNVVSALMELRKR
                     PVNAQGQTVDPVVWECNCMEEVCGACAMVINGVPRQACAALVGELEQPIRLEPLSKFP
                     VIRDLQVDRQKVFDALMKVKAWTPIDGSWDIHRHAPLISPGEQSLRYLFSRCMTCGCC
                     MEACPQYHKDSEFIGPAVLGQVRLHNSHPTGQFYKDERLQAIMEKGGIADCGNAQNCV
                     KVCPKDIPLTQAIGELGRQTTMQWLRNFFVS"
     gene            complement(4010..5896)
                     /gene="sdhA"
                     /locus_tag="PLU99_07585"
     CDS             complement(4010..5896)
                     /gene="sdhA"
                     /locus_tag="PLU99_07585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HOO16955.1"
                     /translation="MAAKPNIIVVGGGLGGLWATLRIAEAGFPVDLFSLFPVKRSHSC
                     CAQGGINAVLDTKGQNDSIWQHIVDTIKGGDYLGEQPPIKSLCEEAPGLIRTYDRMGV
                     TFSRTPEGIMDLRLFGGVKNKRTCFAGATTGQQLLYACDEQVRAHEARGAVRKYEHWE
                     FLSVVLDEEGICRGITALDLNSMKVQAFRADAVILATGGMGLIWGRSTMSTNSTGSAA
                     ARVYQQGAKLANAEFVQFHPTAMKGHDKNRLMSEACRGEGGRIWVPKQAGDTRRPQDI
                     PENERFYFLEEWYPTYGNTVPRDVASRAIVRVTRTMGLGLGGQDVVYLDLSHQPRKFL
                     EDRLGGILDMYRDFSGEDPYTGPMKVFPAMHYSMGGLWVDYQKDEKTGGMARVSPRNH
                     STTIPGLYACGECDYAYHGANRLGANSLLSASFSGRVAGDASVAYVKGLKKRAAATPD
                     NFFKQEQSRQEEINQAIMTRQGGENPFALHHELGEIMRKHVFVERENKGLDIALNGIR
                     KLKERSQRISLDDRSGWANQSLSWGRQVQDMIVLAEVITVGARLRDECRGSHYKAEFE
                     LKVPEGKFAGDPEYEAYKARWKENNEKWMKHTIARHTADGPQITYSPVDVSVMPPETP
                     RDYR"
     gene            6345..7010
                     /locus_tag="PLU99_07590"
     CDS             6345..7010
                     /locus_tag="PLU99_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO16956.1"
                     /translation="MGAFFTPRANLLARGAPVAGVLLLGLVGFVWYGWVNSPYGTRVG
                     YYVWQPVPFSHAHHVGDDGIDCRYCHATAEVSSFAGMPSTETCMHCHSQLWTNAEMLA
                     PVRESWRTDVPLVWNRVHDLADFVFFNHAVHVNNGVGCVTCHGRVDRMPLITKSKSLY
                     MEWCLECHRNPAPYLRPRDQIYSLTWEPPEDHAALAAQLMREYEIDAVRGAALTNCSA
                     CHR"
     gene            7019..9955
                     /locus_tag="PLU99_07595"
     CDS             7019..9955
                     /locus_tag="PLU99_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOO16957.1"
                     /translation="MAKTHDNEVPLWRSLDELARSPEFLRWTEAEFPEQVRAGGLGRR
                     EFLKMAAVSFALAGLGGCGERPPRQIVPYVHPQPAVVPGEAVYFATSVLHAGYALGVV
                     VDSRLGRPTKIEGNPEHPASLGATDIFAQAQIYELYDPDRSQAVYRGGRISAWSQFEP
                     EWRAVLADMRGRGGAGLRLLTGRITSPTALAHISQLLEELPEARWHVYEPIDDTNAQE
                     GLKTAFGQPADLVYSLERADVIFSLDCDFLNDVPGSVRYAREFGRRRTVRQDGINMCR
                     LYVAEPSPTVTGSIADNRLPVRPGLLPAVLGRLAERLGVSEGELSATRGAAALDENVR
                     HWVDVLARDLEQHRGASLVCVGRRLPPQAHALAALCNERLGNVGATLRAIEPVPAPTP
                     AFGGLSDLLKEMQAGAVTHLLIFGANPVYTAPADFAFADELARVPWTAHFGLYRDETA
                     VRCFWHLPLSHTLESWSDARAFDGTAGIIQPLLTPLYQTRSLQAVLAMLAGRPLVGDR
                     ELVRDHWRKTVNEADFESWWREVLRAGVIPNSAAPALDVEVRAEAVRQAWRSAAEPRE
                     DRPNQLELAFAPDPTLWDGAYASNVWLQESPKPITKLSWNNAALVAPQTAERLGLRSR
                     DVVQLTTDNRAVLAAVLPVPGHAPDCLTLPLGYGREVAAAPAATGFNAYALRTSSHPW
                     LTAGAVERTATRQPLISTQMHFALEGRPLLRRATVSELTSAPAIEHSQPRDSLYPEWP
                     YPGTQWAMVIDLTRCNGCSACTIACQAENNIPTVGPDQVQRGREMYWMLVHTYFSGSP
                     DAPGIYHQPRPCMHCEKAPCELVCPVGATQHSADGLNEMIYNRCVGTRYCSNNCPYKV
                     RRFNFYNYTRDDPPVQWLQRNPEVTVRARGVMEKCTYCVQRIRAAEIEARIAERPIPA
                     GRVVPACAAACPTQAIFFGDMNDPESPVAPLKREATNFDMLAELNTRPRTSYLAAIRN
                     PNPELT"
     gene            10062..11318
                     /gene="nrfD"
                     /locus_tag="PLU99_07600"
                     /pseudo
     CDS             10062..11318
                     /gene="nrfD"
                     /locus_tag="PLU99_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
     gene            11319..11933
                     /locus_tag="PLU99_07605"
     CDS             11319..11933
                     /locus_tag="PLU99_07605"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOO16958.1"
                     /translation="MGHPATVTLPTELPAAAELPDQPSNPQGAIAAFLEPHDLVTAVR
                     RTRQAGYSRLNAYTPFPVQDLPQALGLKRTRVPLFVLLGGLAGAIGAFYMQYYSATIS
                     YPWNIGGRPLNSWPSFIPLTFELGVLGGALCGLLAMLIANRLPQLYHPILNAPGFDRA
                     SQDRFLLSIEAADPRFDSRETLAFLHSLQPEYAELVPAESEAYS"
BASE COUNT         2038 a         3703 c         3784 g         2421 t
ORIGIN      
        1 gcgatcttga cttctatcgc tacgagctgt ccgggacgga tccggcatgc acaattctcc
       61 aggccaaggt caggggcgag ttcgagcccg acatctggct gatggtcgga gagtgcttca
      121 ccgatagctg gtgcggcgtt gaggagacct acgtcatggt ctactcgcgt gccgccggcg
      181 gcgtgtgcga ttacgccatc gcggccgccc cgctgcccag cgatccgccg cagtacgtct
      241 acatcatggt ttcctgcttc ggctcggtcg gcgacgtacc gtgcaacacg ccttacacgc
      301 tggaaatcga aaaggtggca gcgggctgct gcaggatcgg cgaagcatcg ggtatcacca
      361 gcgaagccta ctgcctggct ctgggcggcg agtggacggc cggcgactgc tgcggcgagc
      421 aagagtgcgt cctggccgat tctaactgcg acggcgcagt caacgtgttc gatatcgatg
      481 cgttcgtcct ggctctgacc aacccggcgc agtggctagc cacgtacacc tgcgatattt
      541 gcaacaacga ctgcaactgc gacggcgaag tcaacgtgtt tgacatcgac ccgttcgtgc
      601 agtgcctgac ttccggcggc gcctgcacgt gcccatagca gttagcatat ggcaggtgag
      661 cggtcactga ccgctcaccg ggccttgcgg cgacgtcccc tacggcgtcg ctcagcaacg
      721 taccggcggc tgaccgccgg gtgaaacgca aaaacaggcc ggcctgaggt gaaacgcctc
      781 gggccggcct tgctgtttgg atgcctaatg caccgtcttc acgatttaac tgcccggcaa
      841 cgactggtcc ggccgggtga gaagcgcggc aagttgaaaa cgtggtgagc ggaccctgta
      901 cggtggacaa ttccgggcgc agcgcagcca attcatcgcc actcgccctg acaaccaggc
      961 gccgctagcg ccgcgcgatg acccgctgtt cgctttccag gatgagcgcc agggcttcgg
     1021 ctggatcgtc gatgtcccgc aatctggcgg gcaaatccgt gctgagcatc attgccagac
     1081 gagccagcac gcacaggtgc tgccgctcct catgactgca cacgaaaaag aagatgtgcg
     1141 ttagctggcc gtcgggcgct ccgaacggaa tgcccgcggg cacacgcgcc aagcacacca
     1201 gcggctcggc cgtggcgtac ggcagggggc gcctggggtg cggcagggcc acaccgtggg
     1261 gcagcgccgt ggggtatagc tcctcgcgct cctgcacggc cgtcagcagg ccgatcgggt
     1321 cgtaaaccaa accagtccgc tcggcgagag aaatcagctc tttcagcacc gatgtccgcg
     1381 aacgggccgg caagttcata tcgatggctt ccggcggcag aagggcgccg agcacctcct
     1441 cgtcgttgcc gtcggacatg gcgcgttcga gattcgcgat gtgctttgga tcgaggttgt
     1501 gtatctcctg ctggagccaa tccagcattt gggcccggtt aaaccgccac tggccctgca
     1561 ccatcgagcc gggcaggcgg cccttctcgg cccactttcg gacgatgcgc gcatccatgc
     1621 cgatgtgccg cgcaagttca tcaatgctca tgttgcggta gggcatagcc gggcaccggg
     1681 catccgaatc gaaaattgta ccgcagagcg ctcgcgcaaa aaagacaata ttttctgcaa
     1741 aacacgtgtt gggagagagg ctgccgagcc gtacgaacag cgcggcgtct caacctggca
     1801 gcgcgccggg gtccggcagc gcgtattacg atttcgacac gccggcgggc gtgggagcgc
     1861 ccgcgtccgc gcccgcgtac ttcgggacga agatcttctc ggccagcccg tgctgacgag
     1921 cgtaatcgtg caggcgcgga atggtcttca gaacggacaa gtcatcgtgg gtatcggtgc
     1981 cgatcgaaat caacacgcgg tgattcggga ggatgttaaa ccagcgatca tgctcctcga
     2041 gcgggatgaa cttcgaatta agctcgtaga aaatgcgggc gttcgcgagg gtgcgcacaa
     2101 cctgatccat ggatgtcgag gggaattgct gagcgacggc ggcgtgagcc aatcccacgg
     2161 gacacggcca gcgccgggcc tgattggcca gggtggtaag ccccgcccag tccacgtact
     2221 cgaacaagac gtaatcaatg cccgtcagca tggtcttgat cgtgtccagc aggcccttgt
     2281 tcatctgcca gttaatctca gccccgcagt aaaccggcac ccggtccgcc cacttgcggc
     2341 gcgcctcgtg caccgcgttg acgtattctt gggtttcacg atcgaagaca cggtggaact
     2401 cgcgctcgtt gttcacttcg cgcgaagatg gcgtgcagaa aaggtgatcg cagatgccga
     2461 tgccgcccat gttcagctcg ggatcatcca aatgggcctg gatgatctcg tcgatcgtgt
     2521 aggcgccgtc tgagaacggc gtgtggttgt gcaggttata catcagttgg ttcatgcagg
     2581 ctcactccca gccgccatcc cgcgcaggtt gtacgccgcc gatcgtgccc ttagggggcc
     2641 ccgagacctt cccggcccgc cgctacacaa aactgccgtc aggtcagacg gccagtgtca
     2701 agctgaagag ccgaaaaaca tgccccagcc ggcacgtacc ctgaacctgt tcctggcacg
     2761 gccatcaccc aactcagcaa cttagtataa cctgacgcgg acctgcggca agcccatttc
     2821 gggcacgcgt gtgggcctaa cgtgtgaatc agggcatggg gggcgaagct gaccgcggcg
     2881 ccgtcggggc atccggcacg cggctgtcgc ccggatgtga gcgttaggaa tcggatcaac
     2941 tcgcatgctg caggggcttg cccaaggaca gtggcggcag gcgtgcctgc acaacccgct
     3001 gatcttcaca gtagttctga tcgcgccact tgtgttcctg gcgaggggac tgtggcggcg
     3061 ttccagcgta ttgcgccagc gctctatgac gatcgtgtat gtgctgctgg cgacggtatt
     3121 cgtgaactgg gtctacctgc ttttgcgcga acctccctga cgccccgcca gtcgctgcga
     3181 cgggcagacg ttgtcatggc cgcaggccga atccgatagt tgcagccttc agctcacaaa
     3241 gaagttccgt agccactgca tcgtcgtctg ccgtcccagt tcgccgatgg cttgcgtcag
     3301 ggggatgtcc ttggggcaga ccttgacgca gttctgagcg ttgccgcaat ccgcgatgcc
     3361 gcctttctcc atgattgcct gcagacgttc atctttgtag aactggccgg tcgggtgtga
     3421 attatgtaag cgcacctggc cgagaactgc ggggccgatg aattccgagt ctttgtgata
     3481 ctgcgggcag gcttccatgc agcagccgca agtcatgcag cggctgaaca ggtaacgcag
     3541 cgattgttcg ccggggctga tgagcggggc gtggcggtgg atgtcccagc ttccgtcgat
     3601 gggggtccag gccttgacct tcatgagggc gtcaaagacc ttttgccggt caacctgcaa
     3661 gtcgcggatg accgggaact tgctcagcgg ttccaggcgg atgggctgct ccagctcgcc
     3721 gacgagggcg gcgcaggcct gccgtggcac gccgttgatg accatggcgc aggccccgca
     3781 gacctcttcc atgcagttgc actcccagac gaccggatcg acggtctggc cctgggcgtt
     3841 gaccgggcgc ttgcgaagtt ccatcaaggc cgagacgacg ttgtgcccgc cggcgtccgg
     3901 catgatgaaa tcttcccagt aaggctgggc atcggggccg gcttgacgtt tgatacgaaa
     3961 gtgaatctgg ttagccatcg cgaatttcct gcgtgtattt cgattctgct tatctgtaat
     4021 cccgcggcgt ctccggcggc atcacgctca cgtcgaccgg cgaataggta atctgtgggc
     4081 cgtccgctgt atgccgggcg atcgtgtgtt tcatccactt ctcgttattt tccttccagc
     4141 gggccttata agcctcgtat tccggatcgc ccgcgaactt gccctccggc accttcagct
     4201 caaattcggc tttgtaatgc gagccgcggc attcgtcgcg caggcgggcg ccgaccgtga
     4261 taacctcggc cagcacgatc atgtcctgga cctggcggcc ccacgatagc gactggttcg
     4321 cccagccgct gcggtcgtcg aggctgatgc gctgcgaacg ctccttgagc ttgcggatgc
     4381 cgttcagcgc gatatccagc cccttgttct cgcgctcgac aaatacgtgc ttccgcatga
     4441 tctcgcccag ttcgtgatgc agcgcgaacg ggttctcgcc gccctggcgg gtcatgatcg
     4501 cctggttgat ttcttcttgc cggctctgtt cctgtttgaa gaagttatcc ggcgtcgccg
     4561 ccgcccgctt cttcaggcct ttgacgtacg cgacggatgc gtcgccggcc acccgtccgc
     4621 tgaacgatgc gctcagcagc gaattcgcgc ctagccggtt cgcgccgtga tacgcataat
     4681 cgcattcgcc gcaggcgtag aggcccggga tcgtcgtcga atggttccgc ggcgagacgc
     4741 gggccatgcc gcccgttttc tcgtccttct gatagtcgac ccagagcccg cccatcgaat
     4801 agtgcatcgc gggaaagacc ttcatcggcc cggtgtacgg gtcttcgcct gagaaatcgc
     4861 ggtacatgtc gagaatgccg cccaggcggt cttcaaggaa cttccgcggc tggtgcgaca
     4921 ggtcgagata aaccacgtcc tgtccgccga gccccaggcc catcgtgcgc gtaacgcgaa
     4981 cgatcgctcg gctggccacg tcgcgcggca ccgtgttccc gtaagtcggg taccactcct
     5041 cgagaaagta gaaacgctca ttctcgggga tgtcctgcgg ccgccgggtg tcgccggcct
     5101 gcttgggcac ccagattcgt ccgccctcgc cgcggcaggc ctcagacatc aggcggttct
     5161 tgtcgtgccc cttcatggcg gtcgggtgga attgcacgaa ctcagcgttc gccagcttcg
     5221 cgccctgctg atatacgcgc gccgcggctg agccggtcga attggtcgac atcgtagacc
     5281 ggccccagat caggcccatg ccaccggtgg cgagaatgac cgcatccgcg cggaacgcct
     5341 gcaccttcat gctgttgagg tcgagcgcgg ttatgccgcg gcaaatgccc tcttcatcga
     5401 gcacgaccga caggaactcc caatgctcgt acttgcgtac cgccccgcgc gcctcgtgcg
     5461 cccgcacctg ctcatcgcag gcgtatagga gctgctggcc ggtggtcgcg ccggcgaagc
     5521 acgttcgctt gttcttgacg ccgccgaaca gccgcaggtc catgatccct tccggcgtgc
     5581 ggctgaacgt cacgcccatg cggtcatagg tgcggatgag gcccggcgcc tcctcgcaca
     5641 agctctttat gggcggctgc tcgcccaggt agtcgccgcc cttgatcgtg tcgacgatgt
     5701 gctgccagat cgagtcgttc tggcccttgg tgtcgagaac ggcgttaatg ccgccctgcg
     5761 cgcagcagga gtgcgagcgc ttgaccggga acagcgagaa cagatcgacg ggaaagccgg
     5821 cttcggcgat ccgtagcgtg gcccatagcc cgcctaatcc gccgccgacc acgatgatgt
     5881 tcggtttggc tgccattttc gtgctttctc caagagcggc ttgcgctcac aggtgagtgg
     5941 cgccagggcc gccgaatgtc ggaccattat aaggggaaac tcggcgcgtg catgtcggag
     6001 ttgtaatact gttttgggcg aattttcggc actttttgga gcgacggggc cgccggggac
     6061 ccgtcgtggc gtgttgttcg cgcgttttcc tgaacggatc gtaatttgaa atttagaagc
     6121 ttgtcagccc tggctaggaa acttcagcac gtctggtagt gaactcaggc gtaagctcct
     6181 ttaggcgtcc gctgcttgtg ccttttgcgc ggcgaaggcg cgcgccggac gccggatata
     6241 aggaaactgt accccgtttg cctgcgcgag gtgattaggc cctgcgccaa agagacgttt
     6301 caattaagca cgcgctccag cggcgcgggt aagggacgcg ctgcatgggc gccttcttca
     6361 caccccgggc gaatcttctg gcccgcggcg cacccgtcgc tggtgtgcta ctgctcggcc
     6421 tggtgggctt cgtctggtac ggctgggtta actcgccgta tggcacgcgc gtcggctatt
     6481 acgtctggca gcccgtgccg ttcagccatg cgcaccacgt cggagatgac ggcatcgact
     6541 gtcgctattg ccatgctacc gcagaagtct cttcgttcgc cggcatgccg tcgacggaaa
     6601 cgtgcatgca ctgtcattcg cagctctgga cgaatgccga gatgctcgcg ccggtgcggg
     6661 agagctggcg gaccgacgtg cccctggtct ggaaccgcgt gcacgatctg gccgacttcg
     6721 ttttcttcaa ccacgcggtc catgtcaaca acggcgtggg atgtgtgacg tgtcacggcc
     6781 gcgtggatcg catgcccctg ataacgaaga gcaagtcgct ttacatggaa tggtgcctcg
     6841 agtgccaccg caacccggcc ccgtatttac ggccacgcga ccagatttat agcctcactt
     6901 gggagccgcc cgaggaccac gccgcgctcg cggcgcagct catgcgcgaa tacgagattg
     6961 acgcagtgcg cggcgcggcg ctgaccaact gctcggcttg tcatcgttag gagcgagagt
     7021 ggcgaaaacg cacgataacg aggttcctct ctggcgcagc cttgacgaat tggcccgctc
     7081 gccggagttc ctgcgctgga cggaagcgga gttcccggag caagttcgag cgggaggctt
     7141 ggggcggcgc gaatttctca agatggcggc ggtgtctttc gcactggccg gcttgggtgg
     7201 gtgcggcgag cgtccgccgc ggcagattgt gccgtacgtg catccgcagc cggccgtggt
     7261 gccgggggag gcggtgtact tcgcgacctc cgtgctccat gccggttacg cgctgggcgt
     7321 ggtggtggat agccgattgg ggcgaccgac gaaaatcgaa ggtaatccgg aacatccggc
     7381 cagtctgggt gcgactgaca tttttgcgca ggcgcagatt tatgaactct acgacccgga
     7441 tcgttcgcaa gccgtttacc gcggcgggcg aatcagcgca tggagtcagt tcgagccgga
     7501 gtggcgcgcc gtgctcgccg atatgcgggg acgcggcggg gcgggactgc gactgctgac
     7561 cgggcgcatc acttcgccga ccgcgctggc acatatatcc cagcttctgg aggagcttcc
     7621 ggaggcgcgc tggcacgttt acgagccgat tgacgacacc aatgcacagg agggactcaa
     7681 aaccgcgttt ggccagccgg ccgatttggt ctattcgctc gagcgtgcgg acgtcatctt
     7741 ttcactggat tgcgacttct tgaatgacgt tcccggcagt gttcgctacg cgcgcgagtt
     7801 cggccgccgc cgaacggtgc ggcaggacgg catcaacatg tgccggctgt acgtcgccga
     7861 gccctcgccg actgtcaccg gctcgatagc ggacaatcgg ctgccggtgc gacccgggct
     7921 gctgccggcg gtactcgggc ggctggcgga gcggctggga gtgtcggagg gcgaactgag
     7981 cgccacgcgc ggcgctgcgg cgctggacga aaacgtgagg cattgggtcg acgtgctggc
     8041 gcgcgacctg gagcagcacc gcggcgcatc tctggtctgc gtcggtcggc gtctgccacc
     8101 gcaggcgcat gcgctggcgg cgctgtgcaa tgagcgactc gggaacgtcg gcgcgacgct
     8161 gcgcgccatc gagcccgtgc cggcgccgac cccggccttc ggcggcctgt ccgacctgct
     8221 taaggaaatg caggccggag cagtgacgca cctgctcatc ttcggggcca acccggtgta
     8281 caccgccccg gccgattttg cgttcgcgga cgaactggca cgcgtcccgt ggacggccca
     8341 ttttgggctc taccgcgacg aaaccgcggt gcgctgcttc tggcatctgc cgctatcgca
     8401 cacgctggaa tcgtggagtg acgcccgcgc tttcgacggc acggccggca tcattcagcc
     8461 gctgttgacg ccgctgtacc agacgcggtc attgcaggcc gttctggcaa tgctcgccgg
     8521 ccggccgctg gtcggggatc gcgaactcgt acgcgaccac tggcgcaaaa cagtgaacga
     8581 agccgacttc gagtcgtggt ggcgcgaggt gttgcgggcc ggtgttatcc cgaattccgc
     8641 tgcgccggcg ctcgatgtgg aagtccgggc cgaggccgtg cgtcaggcct ggcgcagcgc
     8701 agcggagccg cgcgaagatc gtcctaatca gctcgaactg gcctttgccc ccgacccgac
     8761 gctttgggat ggggcgtatg ccagcaatgt gtggctgcag gaatcgccca agcccatcac
     8821 gaagctgtcc tggaacaacg ccgcgctcgt cgcgccgcag acggccgaac ggctgggact
     8881 gcgaagccgt gacgtcgtgc agcttacaac ggataatcgt gccgttttag cggctgtgtt
     8941 gccggttccc gggcacgcgc ccgactgcct cacgttgccc ttgggctacg gccgcgaggt
     9001 tgctgccgcg ccggccgcga ctgggttcaa cgcctacgcc ctgcggacct cgtcacatcc
     9061 gtggttgacc gccggcgctg tcgagcgcac cgccacgagg cagcctctga tatctacgca
     9121 gatgcacttc gcgctggaag gccgtccgtt gctgcgccgc gccactgtca gcgaactcac
     9181 aagcgcgccc gcgatcgagc attcacagcc gcgcgattca ttgtatcccg agtggccgta
     9241 tccgggtacg cagtgggcca tggtgattga cctgacgcgc tgtaacggct gcagtgcgtg
     9301 cacgatcgcc tgccaagccg agaacaacat cccgaccgtt gggccggacc aggtgcagcg
     9361 cgggcgcgag atgtactgga tgctggtgca cacgtatttc agcgggtcac ctgatgcgcc
     9421 ggggatctac catcaaccgc ggccctgtat gcactgcgag aaggcgccct gtgaactggt
     9481 atgtccagtg ggcgcgaccc agcatagcgc tgatggtctt aacgaaatga tatataatcg
     9541 ctgcgtcggc acacgctact gctcgaacaa ttgcccctac aaggtgcgcc gcttcaattt
     9601 ttacaactac actcgcgatg atccgccggt gcaatggctg caacggaatc cggaggtgac
     9661 agtgcgggcc cgcggcgtaa tggagaaatg tacctattgt gtacagcgca tccgggcggc
     9721 cgaaatcgaa gcccgcatag ccgaacggcc cattccggcg ggccgcgttg tgccggcctg
     9781 cgctgcggct tgcccgacac aggccatttt cttcggcgat atgaatgacc cggaaagccc
     9841 ggtggcgccg ctcaaacgcg aggcgaccaa ctttgacatg ctcgcggaat tgaacacgcg
     9901 gccgcgcacg tcgtatctgg ccgcgatccg taacccgaat ccggaattga cgtagctccg
     9961 tgtcaagatc gagttccata tcggccggtg gcggccagtc gaccagcgac gtgatcgact
    10021 cgcgactcac ttacgcggat gtcacgcgac gcgtcgtcgc aattccgctc ggacgtacgt
    10081 cgtggacctg gctgagccta tttggattcg ccatggccgc ctccggactg ctggccgcgg
    10141 gcatgttcgt ggtgttgacc tggggtgtcg gcgtgtgggg ggtcgacatc ccagtcgcgt
    10201 ggggctttgc catcatcaat ttcgtttggt ggattggaat tggtcacgct ggcactctga
    10261 tttcagcgat tcttttgttg ttgcggcagg actggcgtac gtcgattaac cgctttgcgg
    10321 aggcgatgac gctgtttgcg gtcgcgaatg cgggcttgtt tcctcttctg cacttgggcc
    10381 ggcagtggaa gttctattac ctgctcccct atcccgacac gctgggagcg tggccacagt
    10441 ggcgcagtcc gctggtatgg gacatcttcg ccgtactaac ctactttacc gtgtccctga
    10501 tattttggta cgtcggtttg ctgcccgatc tggcgacttt gcgggacatt ggccggtcgc
    10561 ggcgggtgaa acgctgggca ggcatctttt cgttaggctg gcgcggcgat gcaacccact
    10621 ggcaacgcta ccagtcgctc tacctgctgc tcgccggtct ggccacgccg ctggtcgtgt
    10681 ccgtacacag cattgtgtcc cttgatttcg ccgtcggcat tgttcccggc tggcacacca
    10741 cggtctttcc cccgtatttc gtagccggtg caatctactc cggcttcgcc atggtattcg
    10801 tgctggcgct gccgttgcgc accgcctacg gcttgaaaga cctgatcacg acccgccacc
    10861 tgaatgccat gggcaaggtc atgttggctt cagggctgat cgtggcttac ggctacctga
    10921 tcgagaattt cagcgcgttt ttcagcaacg acgaatttga gatcgcacag gcgaaaacgc
    10981 gctggtttgg accatatgct gtcctatatt ggggaacgct tatctgcaac gtcgccattg
    11041 gaaatttgct gtggtctcgc tgggtgcgcg tgcacgcgct gccgcttttc ttagtcagcg
    11101 ccgcggtgct tttgggcatg tggctcgaac gatttgtcat tatcgcagtg agcctgcatc
    11161 gcgattatgt gccggcggcg tggggcatgt attacccgac tatctgggac tgggcgttgt
    11221 tactgggttc gctggccttc tttctatttc tgtttctact gttcattcgc cttgtgccca
    11281 tgattgccgc ctctgaagtc cgcgaactgc tgcatgaagt gggccatccg gccacggtca
    11341 ctctaccgac agaattaccg gccgccgccg aactcccgga ccagccgagt aacccgcagg
    11401 gcgccatcgc agcttttctg gagccccacg acctggtgac cgccgtacgc cggacgcgcc
    11461 aagccggcta cagccgcctg aacgcgtaca caccctttcc ggtgcaggac ctgccgcagg
    11521 ccctgggact gaaacgtacg cgagttccgt tgtttgtgct actgggagga ttagccggag
    11581 ccattggtgc cttttacatg cagtattact cggccaccat cagttacccc tggaatatcg
    11641 gcgggcgccc gctgaacagt tggccgtcgt tcatcccgtt gaccttcgag ttgggggtgc
    11701 tcggcggggc cttgtgcgga ctgctcgcaa tgctgatcgc aaaccgtttg ccgcagctct
    11761 atcaccccat tcttaacgcg cccgggtttg atcgtgcttc acaagaccgc tttctgcttt
    11821 cgatcgaagc cgccgacccg cgcttcgact cacgcgagac gctcgctttt ctgcacagtc
    11881 tgcagcctga gtacgcagaa ctggtgccgg ccgaatcgga ggcttattca tgaggcgctg
    11941 cgcatg
//
LOCUS       DAOSYS010000096        11906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4557_length_11906_cov_6.047000, whole genome shotgun sequence.
ACCESSION   DAOSYS010000096 DAOSYS010000000
VERSION     DAOSYS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_11906_cov_6.047000"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLU99_07610"
     CDS             complement(<1..825)
                     /locus_tag="PLU99_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16959.1"
                     /translation="MCVSRVAARACVLVAVGLLSSTAAWAIQAGIVFDVHQDIRDPDL
                     WPNDFHVEGKLQSLVGLPTVINHLDGPFTKFKWEITPLGGEDYWFECTWWMEEPGVYI
                     PYCEILHMGVLFDVDYKNIVIDVVSWWTRDGKPVGEKARGALYNDGFVPMIGFNVADG
                     SEPQLLTIANNFVTATPPPPPAEQPPPWPPTPIKLDIVQLQAAVFDPDHEPKFEDLAE
                     GGASEGYKWVDIVRADGSKISGDRPLSLDAGKVIDVILGEARSDADMLRAAEPIKIE"
     gene            complement(1268..2233)
                     /locus_tag="PLU99_07615"
     CDS             complement(1268..2233)
                     /locus_tag="PLU99_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16960.1"
                     /translation="MAEAVHGPTSNWAATPPVRTARPVSWGAIFAGAVLVTVIQLMLG
                     LLGLAIGLAAVEPGKGEGYFAGLGIAGYIWWLASGIVALFIGGWASARLAGAHRRTNG
                     ALHGLVVWGLATLFMAYLLTTTAGNIMGGAFNLLDQTVSYGARAVTGTGAEATADSKT
                     ADSKTADGKAADGGTANGGKTPEQVYSQSQTTLQRWQATEGAWDQLKPALGNYFDADG
                     ATAQDKERVIEILVSQTSMSRPEAERNAEQWAQGAQGLQAAAERAGEVAEEWGGKVAD
                     WSAAASFWAFIMLLIGAGAAAFGGSLGIYRTDRFGHDEPVTPPRA"
     gene            complement(2405..2821)
                     /locus_tag="PLU99_07620"
     CDS             complement(2405..2821)
                     /locus_tag="PLU99_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOO16961.1"
                     /translation="MTPQARQCERCHRTVEQRGPTPVRFCPVCGQALSESAQPAGAET
                     SAARSDTRSRTSVQAVLGLILAVVSLVIQPCAFPLGLISVLLGLTAQKSIERSAGLLV
                     GRGYALAAVVLGLISLIVWLIINVFVRPAHYEYASF"
     gene            complement(2832..3521)
                     /gene="deoC"
                     /locus_tag="PLU99_07625"
     CDS             complement(2832..3521)
                     /gene="deoC"
                     /locus_tag="PLU99_07625"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOO16962.1"
                     /translation="MPSGFPNTRAALARCIDHTLLKPEATAADIEKLCVEAREYGFFG
                     VCVNPIWVPRCAELLRGTPTCVVSVAGFPLGASTTSVKALEAKLAVEQGAAEVDMVVN
                     LAALRAGDREAVIRDIAEVVAAVKRVNERALVKVILETRALTTEQIILGCRCAAEARA
                     DFVKTSTGFHPAGGATVAHVALLHQHAAPLRVKAAGGIRDAASALAMIEAGAARLGLS
                     ASVAVLRELRA"
     gene            complement(3629..5395)
                     /locus_tag="PLU99_07630"
     CDS             complement(3629..5395)
                     /locus_tag="PLU99_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16963.1"
                     /translation="MAAGSIIDRLSDPALVAVCADAEALPRSILSPAVVVPDQWVALI
                     ERVDGRRRLVPAGEEPRPQRDEIILFARNRPIKVPLSVEGAAADKHDVTLTGELLVRW
                     QARADELATLRRTLLDSGQLTVEQLAERIAAAGAEPALQRFLRENPAARLVHEDVREE
                     LEGVLREALKRFLFTTGMTLEGVRALRCESFSLNRKEALERETAARLAGIESRALVEQ
                     AALAATERRLNHLSGLLEKMKAAAGTSEDLQWRDLLPSLSPGERGRLLESLWRISPDR
                     QVAAALIAVAGNDCLWFDPERVEQPLRVVHLDDSLGGLRSVTYCPQHDLLLVGAARGV
                     WTISAADGSALAAYPVKSSEAPRTGFNAAAVAGDLLFASHSQLGVWAWPLSAPEAGEQ
                     RYEPIAGTPKTIRAVVPAGDRVLFAADDVVHMLALANDDHELSPPAPEVIQCLAVDGR
                     TVYAGVTDGVLASLNIDRPDGWVPLHRATSGFESIVLRHWSDLLELVIPAGSAGVQGV
                     YPQQGIVARLMESTVPIRRVWASDDLLVALSENRDRLLVMTQDAPDRRGREIPIGRML
                     GRSIQDATIITTTEAAESAETH"
     gene            complement(5404..5775)
                     /locus_tag="PLU99_07635"
     CDS             complement(5404..5775)
                     /locus_tag="PLU99_07635"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HOO16964.1"
                     /translation="MSHVASGVSGPQIRCPDSDCGWRNQAGARYCASCGRPLSDYRRF
                     RLGELTVRSVGMAYLLWALCLVGAAGIHRFYTGRYVTGVIWLLTLGLVGIGSLVDLFL
                     IPGMVESKNRELARRGSWQFP"
     gene            complement(5833..6954)
                     /locus_tag="PLU99_07640"
     CDS             complement(5833..6954)
                     /locus_tag="PLU99_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO16965.1"
                     /translation="MPATFATFKRLKDKGLAALREGDYTAARPYLIQAAECMIELAEA
                     NDDPKHREEQEAYAKELLELARSCDRRVGAPGGSPRARDRAREEENGAQAEDWIVREK
                     PNVRFADIAGLEDVKQEIYLKMIYPYRHPELAEHYGIDVGGGLLLFGPPGTGKTMIAK
                     AVATEVDATMFVISPAQIMSKWVGEAEQNVRKLFEAAKAEPKSVIFMDEIEALVPRRR
                     SSESSVMQRVVPQILQELEGFDRKAMRALMFLGATNEPWSLDPAILRPGRFDSKVYVP
                     LPDAEARFRLFEIYVGKRPLADDVNLAELVEKTAGYSGADIKAVCGRAATRPFLESVA
                     GAEPRKIAMADLLAAIEDVPPSVGAKDHQKFEEWAATNS"
     gene            complement(6954..7358)
                     /locus_tag="PLU99_07645"
     CDS             complement(6954..7358)
                     /locus_tag="PLU99_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOO16966.1"
                     /translation="MPPKEELARGWLVKAQRDLEMANRALEEEPRLPDMACFHSHQTI
                     EKALKAVLLYRGIQAPRTHVLDTLLKLCGPIDPRFDLLAPQLSLMTEFAVEGRYPDTG
                     CEPNLDEAREALRLAREAYDIIVSALPTEVLP"
     gene            complement(7348..7734)
                     /locus_tag="PLU99_07650"
     CDS             complement(7348..7734)
                     /locus_tag="PLU99_07650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO16967.1"
                     /translation="MKGERPGTRQTPDGYGDAAPGVMPEFSQDMLAEIIRRLVAALTP
                     RAIYFFGSHAYGTPHRDSDIDLLIVLDHIDSIMDVARRGYGALFGLGLPIELHFITAE
                     KLERYSGVIGSFHREVKTRGRIVYAA"
     gene            complement(7731..8342)
                     /locus_tag="PLU99_07655"
     CDS             complement(7731..8342)
                     /locus_tag="PLU99_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16968.1"
                     /translation="MGLFKSKEERRLERDMRIRQGIRRIEKSIAEQNKFTDEFVKNAR
                     QAKQIGDQAQYQFIRSSLKKTATIKKLLERQLLAVKNALLIKRQAEASADFAEAMSLM
                     AREVSRMYGDTDLAKTQMDWERALMQSQSMEERMQMFLDAIEQGAAADVSAAGTTEAI
                     SDEEIDRMIAAEEQVEAARESQQMSALRAELAALKGEGQKETK"
     gene            complement(8433..8858)
                     /locus_tag="PLU99_07660"
     CDS             complement(8433..8858)
                     /locus_tag="PLU99_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOO16969.1"
                     /translation="MAARRFEDLRVWQTARDLTRGVYRASENQKLRGDFALASQMKRA
                     AVSIGSNIAEGFERGTRKQQTEYCYTAKGSAAELRSQIITAYDVGLINQHVYEWLIDK
                     AELCARQLQAYIIHLRGTRKTLPGPKYLACEDQQGERVP"
     gene            complement(8903..9511)
                     /locus_tag="PLU99_07665"
     CDS             complement(8903..9511)
                     /locus_tag="PLU99_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16970.1"
                     /translation="MNFFEWLTTKRKTLATMNVAELRAQEMLLQNERNRMQARVGQLA
                     KDKQKIIAQGAKEKTPELRRTFAQQYDLLHTEQMMIARHLNIRSKEALTVSRLRMLRE
                     SGQMFGGAAGVRPLIRESDLAAIERLIENDRITSEMYQERLDEILRMGAEADESTAGI
                     SPAAQELLKIWNDMDAGLIKDTGTAFDEAERRVREREKAADT"
     assembly_gap    9533..9632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9633..10978)
                     /locus_tag="PLU99_07670"
     CDS             complement(<9633..10978)
                     /locus_tag="PLU99_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16971.1"
                     /translation="MNTIWTPMDERSRQRQFEDSFHRQLRARATKLIHGSLPADRVVV
                     ESMPDGRDAVRATLNRLEMYDLDLLNELPGTRAVSLEFGRQLIWPLSYTVARVRAQVL
                     TPTAALVAGETPGPIGRDQVQYALDRYELLPTSQRPTAVILASATGFTPEARAMVTDS
                     DLPKLVLVGGREDGGWDVEMSDSVRYSPWAQLVEFETQDDHVRRLLYHLDKEAAALES
                     RGIALSELSDKLGLPLEQTEAAVRQACRSDPRLMTVVHEGELHVARSPLADEVGAMSL
                     WMRIRRLFGFKPSTAERVRIMTAQRVQLEQQREELDRRLDALEAEERESLQKGAAAAT
                     EAEKKQLAGKLVRVRRDLRRIRAQVNVFTQQIDIIGTHVHNLTLAEQGRRLELPKAED
                     LTREAAEAEQIVAELAANADLAAGIEVGAQTPLQADEEDAILAEFAQVAEAQAAAKT"
     gene            complement(10950..11234)
                     /locus_tag="PLU99_07675"
     CDS             complement(10950..11234)
                     /locus_tag="PLU99_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16972.1"
                     /translation="MLLAEAFHVTVGVLFVLGVLGLIVVAVALVVRTLGFVFRAVAGI
                     GGGDRQLGAARRGRLICPEPRCGHANPGDARYCARCGRSFQHEHDLDAYG"
     gene            complement(11241..>11906)
                     /locus_tag="PLU99_07680"
     CDS             complement(11241..>11906)
                     /locus_tag="PLU99_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO16973.1"
                     /translation="KPGNVLLDLGERPITDVRVADVKIGDFGLGVSNADTLRSIAHSA
                     SLDRDDKLVGTLAYMAPELRDGHYKPSPLSDLFSIGVILFELLTGERPAGAELPSSVR
                     PEVPTALDEVYRRLYARQDSRYESARAVLNDMDARLAAVRPARGGIVPPPPPSGWNAD
                     ASGALRACSSCNHLVAADDQFCTYCGHQLTSRVRRCPACRGYPAARDRFCIFCGTALP
                     LEE"
BASE COUNT         2111 a         3946 c         3461 g         2288 t
ORIGIN      
        1 ctcgatcttg atcggctcag ccgcgcgcaa catatcggcg tccgagcgcg cctcgcccaa
       61 aatcacgtcg atgaccttcc cggcgtcgag gctcagcgga cggtcgccgc tgatcttgct
      121 gccatccgca cgtacgatgt cgacccactt gtaaccctcg gacgccccac cttcggccaa
      181 atcctcaaac ttgggctcgt gatcggggtc aaacacggcc gcctgcagtt gcacaatatc
      241 cagcttgatc ggcgtcggcg gccagggtgg aggctgctcg gccgggggcg gcggcggcgt
      301 cgcggtgacg aagttgttgg cgatggtcag caactgcggc tcgctgccgt cggcgacgtt
      361 gaacccaatc atgggaacga agccgtcgtt gtacaacgcg ccccgggcct tttccccaac
      421 cggcttgccg tcgcgcgtcc accaggacac cacgtcgatg acaatgttct tgtagtcaac
      481 gtcgaagagc acgcccatgt gaaggatttc acagtacgga atataaactc cgggctcctc
      541 catccaccac gtgcactcaa accagtaatc ttccccaccg aggggcgtga tctcccactt
      601 gaacttggtg aaggggccgt ccaagtgatt gatgaccgtg ggtaatccca ccaatgactg
      661 aagtttcccc tcaacgtgaa aatcgttcgg ccacaagtcg gggtctctga tgtcttggtg
      721 tacatcgaag actattcccg cctgaatggc ccaagccgcc gtgctgctta gcaacccgac
      781 ggccaccaag acacatgctc ttgccgctac tctgctgaca cacatacttg ttacctcctc
      841 gggagtgtgc ttatggctcc aaaccaatca cgcattggat tggagccacg tccgccctta
      901 taacagcaga cttaagtttg tcacgcattt tttgcatgcg cttttgcgca aactcacata
      961 ttgttggtta agattgcgca ctcgcgcaaa gtaaagagcg caccagtagc attgtgttgc
     1021 gcattcgcgc aaatactcgc ttgccaaggt cggctttggt acgcgctagg cttgttttcc
     1081 gcctcgcagc gtcgtcgatg tgaaaatcag gattcgtagc tcttttaata atcccgcttt
     1141 aagacacgaa tgtccagtgt gtgccctcta taggcactca cctgtagtat cccaggcaaa
     1201 tacgtatagt tgacttccgc gcgcaccatg cccactcact gcgcgcgagc tgtgtgtccc
     1261 agccgcatta tgcgcgtggc ggcgtgaccg gttcgtcgtg accaaagcgg tccgtgcgat
     1321 agattccgag gctgccgcca aacgccgcgg cccctgctcc aatcaacaac atgatgaaag
     1381 cccagaacga ggctgccgcg ctccaatcgg cgaccttgcc gccccattct tcggccacct
     1441 ctccggcgcg ttcggctgcc gcttgcaatc cttgggcgcc ttgagcccac tgctcggcgt
     1501 tgcgctcggc ctcggggcgg gacatgcttg tttggctgac gaggatctcg attacgcgct
     1561 ctttgtcctg cgctgtggcg ccgtccgcgt cgaagtaatt accgagtgcg ggtttcaact
     1621 ggtcccaggc gccttcggtt gcctgccagc gctgcagcgt agtctgtgac tggctgtaaa
     1681 cctgttcagg cgttttgcca ccgttcgcag ttccgccgtc agcagcttta ccgtcggcag
     1741 ttttgctgtc ggcggtcttg ctgtcggcgg ttgcttcggc gcccgtgccg gtaaccgcgc
     1801 gcgccccgta ggagacggtc tggtccagca ggttgaacgc gccgcccatg atgttgccgg
     1861 ccgtagtggt cagcaggtat gccatgaaga gcgtggccag gccccacacc accagtccat
     1921 ggagagcgcc gttagtgcga cggtgcgccc ccgccaggcg tgcgctcgcc cagccaccta
     1981 tgaacagcgc gacgatgccg gaggccagcc accagatgta gccggcaata cccaacccgg
     2041 cgaagtaccc ctctcctttg cccggttcga ccgcggccag cccgatcgcc aggcccagca
     2101 ggcccaacat aagctggatg acggttacga gcactgcgcc cgcgaaaatc gcgccccacg
     2161 aaaccgggcg cgccgtccgc actgggggtg tagcggccca gtttgatgtt ggtccgtgta
     2221 cggcctcggc catggaaaag cctcctgtgt gaatctccgc cctgaagcag cggccccggg
     2281 tgcccactga tgattgccgg cgcttgtgaa aaagggctga ctgaagtgta aattcggcgt
     2341 caccagagta agcctggggg ccaaatgggc ctcggactac accccaaatt ccagcacaca
     2401 cagttcagaa gctggcgtat tcgtaatgcg ccggcctgac aaacacattt atgatgagcc
     2461 agactatcag gctgattagg cccagcacga ctgccgccag ggcataaccc cggccgacaa
     2521 gcaggccggc cgagcgctcg atactttttt gagctgtcag tcccagcagt acagatatca
     2581 ggcccaacgg gaaggcgcac ggctgaatga ctagcgatac cacagccagg atcagcccca
     2641 gaacggcttg aacgctggta cgcgagcgcg tgtcgctacg ggccgcgctg gtttcggcgc
     2701 cggcgggctg agctgattcg ctcagcgcct ggccgcatac tggacaaaat cgcacgggcg
     2761 tcggcccgcg ttgctcgacc gttctatgac aacgctcgca ctggcgtgcc tggggcgtca
     2821 tggcggctcc atcaggcccg aagttcccgc agcacggcca cactggcaga cagccccagt
     2881 cgcgctgcgc ccgcctcgat catagctaac gcggaggcgg catcgcgaat tccgccggcc
     2941 gctttaacgc gaagtggggc cgcatgctgg tgcaaaaggg ccacgtgtgc gacggtcgcg
     3001 ccgccggccg gatgaaatcc ggttgaggtt ttcacaaagt cggctcgcgc ctcggccgca
     3061 cagcggcagc cgaggattat ttgctcggtt gtcagcgcgc gtgtttcaag gatgactttc
     3121 accagtgcac gttcgttcac cctctttacg gccgccacga cttcggcgat gtcgcgtatg
     3181 acggcttccc ggtcgccggc tcgcaatgcg gcgagattta caaccatgtc gacctcagcg
     3241 gccccttgct cgacggccag tttcgcctcg agagccttga ctgacgtcgt ggacgcgccg
     3301 agtggaaagc ccgccacgct caccacgcat gtaggcgtgc cgcggagcaa ctctgcacag
     3361 cgtgggaccc aaatcggatt aacgcacacg ccgaagaagc cgtactcgcg cgcctcgacg
     3421 cagagttttt caatgtcggc cgcagtcgcc tcaggcttca gcagcgtatg gtcgatgcag
     3481 cgggccagag cggcgcgcgt gttcgggaag ccggatggca attgtgaatc ctccgtgaaa
     3541 agggtgataa ggtgatagag tgataaagtg ttccgcagac catcctttat cactctatca
     3601 ccttatcact ttatcactag tggcatgctc agtgggtttc ggcggattcg gcggcttcgg
     3661 tcgtggtgat aatcgtcgca tcctgaatcg accggcccag catgcgcccg atggggattt
     3721 cgcggccgcg gcggtccggc gcgtcctggg tcatcaccag cagccggtcg cggttctcgc
     3781 tcagcgcgac cagtaggtcg tcgctggccc aaacgcgtcg tataggcacg gtactctcca
     3841 tcaaccgggc cactatgccc tgttgcgggt agacgccttg gactcccgca gacccggcgg
     3901 gaatcaccaa ttccagcaga tcgctccagt gccgcagaac tatggactca aaacccgagg
     3961 tggcccggtg tagcgggacc cagccgtcgg ggcgatcgat gttcagcgaa gccagcacgc
     4021 catcggtcac cccggcatag accgtgcggc cgtcaacggc caaacactga ataacctccg
     4081 gcgccggcgg cgagagctca tgatcatcgt ttgcgagggc taacatgtgc acgacgtcgt
     4141 cggctgcaaa caacacgcgg tcgccggccg gcacgacggc ccggatagtt ttgggcgtcc
     4201 cggcaatcgg ttcgtagcgc tgctcgccag cctccggagc gctaagcggc caggcccaca
     4261 ccccgagctg cgagtggctg gcgaacagca agtcacctgc cacagcggcg gcgttgaaac
     4321 cggtgcgcgg cgcctcacta ctcttgacgg gataagcggc taaagcggac ccatccgcgg
     4381 cgctgatcgt ccagaccccg cgcgccgcgc cgaccagcag cagatcatgc tgcgggcagt
     4441 aggtaaccga acgcagcccg cccaggctgt catccagatg cacgacccgc aacggctgct
     4501 cgacgcgctc cgggtcaaac cacagacagt cgttgcccgc cactgcgatt aacgcggcgg
     4561 ccacttgccg gtcaggcgaa atgcgccaca gactttccag cagccggcca cgctcgccgg
     4621 gcgaaagtga gggcagcagg tcgcgccatt gcaggtcttc gctggtcccg gccgcggcct
     4681 tcatcttctc gagcaacccg ctcagatggt tcaggcggcg ttcggtggcc gccagcgcgg
     4741 cctgctcgac cagggcgcgc gattcgatgc cggccaggcg agcggccgtc tcccgctcga
     4801 gcgcttcttt gcgattaagg gaaaaactct cgcaacgcag tgcccgcacg ccctccagcg
     4861 tcattcccgt ggtgaagagg aagcgtttca acgcctcgcg cagtacgccc tccagctctt
     4921 cccgcacatc ctcatgtaca agccgtgcgg ccggattctc gcgtagaaac cgctgcaggg
     4981 ccggttcggc cccggccgcg gcgatgcgtt ccgccaactg ctccaccgtc aattggccgc
     5041 tgtccaacaa cgtccggcga agagtagcca gttcgtcagc acgcgcctgc cagcgcacca
     5101 gcaactcgcc ggtcaacgtc acatcgtgct tgtccgccgc ggcgccctcg accgaaagcg
     5161 gcaccttgat aggcctgttc cgcgcgaaca ggatgatttc gtcgcgctgc gggcgaggct
     5221 cttcccccgc gggcaccaag cgccgccgtc cgtccacccg ctcgatcaaa gccacccatt
     5281 ggtcgggcac gactacggcc ggtgacaata tcgaacgcgg taaggcctcc gcgtccgcac
     5341 atacggccac gagcgccgga tcactcagcc gatcgatgat gctgcctgct gccatggact
     5401 ttcctatgga aactgccagc ttccgcggcg ggcgagctcc cgatttttac tctccaccat
     5461 gccgggaatg agaaacaggt ccaccagcga tccaatgccc accagaccca gcgtcaacaa
     5521 ccaaatgacg cccgtgacat aacggcctgt gtagaaccgg tgtatgcctg ccgcgcccac
     5581 caggcacagc gcccacagca gatatgccat tcccacagag cgcacggtca gttctcccag
     5641 gcgaaatcgg cggtaatccg acaggggccg cccgcaactg gcgcagtacc gcgccccggc
     5701 ttgattccgc cagccgcagt ccgaatccgg acaacgtatc tgcgggccgg atactccgct
     5761 tgcaacgtgt gacattccgt tcagcctcta ttccggcgac gcccggacga ttccggccag
     5821 ccggcactga ttctagctat tggttgcagc ccactcctca aacttctgat ggtcctttgc
     5881 acctacggat ggcgggacat cctcgatcgc cgcaagcagg tcagccatcg caatcttacg
     5941 cggctccgcc ccagccaccg actcgaggaa aggtcgcgtg gccgcacgcc cgcatacggc
     6001 cttgatatcc gcgccgctgt agcccgcggt tttttcgacc agttccgcga gattcacgtc
     6061 gtcggccaac gggcgtttgc cgacgtagat ttcaaatagt ctaaagcggg cctccgcgtc
     6121 aggcagcggg acgtagactt tggaatcgaa gcggcccggg cgcagaatgg ccggatccag
     6181 gctccacggc tcgttggtgg cgccgaggaa catcagggcc cgcattgctt tccggtcgaa
     6241 gccttcaagc tcctgcagaa tttgaggcac gacgcgctgc atgacgctcg attcagacga
     6301 acgccggcgg gggaccaggg cctcaatttc atccatgaag atgacgcttt tcggttcggc
     6361 cttggcggct tcgaagagtt tgcgcacgtt ctgttcggcc tcgccgaccc acttgctcat
     6421 gatctgcgcc gggctgatga cgaacatggt ggcgtccacc tcggttgcga ccgccttggc
     6481 aatcatggtt ttgccggtgc cgggcggacc gaagagcagc aagccgccgc cgacgtcaat
     6541 gccgtaatgc tcggcgagtt ccgggtggcg atagggatag atcattttca ggtagatttc
     6601 ctgcttgacg tcctccaggc cggcgatgtc ggcaaagcgt acgttgggct tttcgcggac
     6661 gatccaatct tcggcctggg cgccgttctc ctcttcgcgg gcccggtcgc gagcgcgcgg
     6721 actcccgcca ggggcgccga cgcggcggtc gcagctcctg gccagctcga gcagttcctt
     6781 ggcataggcc tcctgctcct cgcggtgctt ggggtcgtcg ttggcttcgg cgagttcaat
     6841 catgcactcg gcggcctgaa tcagatacgg ccgcgcggcg gtgtagtcgc cctcgcgcag
     6901 tgcggccagg cccttgtcct tgaggcgttt gaacgtggca aaggtggcgg gcatcagggg
     6961 agcacctcgg ttggtagcgc gctgacgatg atgtcgtacg cctcgcgggc gagtcttagc
     7021 gcctcgcgcg cctcatccag gttcggttca catccggtgt ccgggtagcg gccctctacc
     7081 gcaaactcgg tcatcagcga aagctgcgga gccagcaagt caaaacgcgg atcgatcggc
     7141 ccgcacaatt tcaacaacgt gtcaagcaca tgtgttcgtg gggcttggat accgcggtac
     7201 aaaagcaccg ccttaagcgc cttttcaatc gtctgatgac tgtggaagca cgccatgtct
     7261 ggtaatcgag gctcttcctc gagcgcgcgg ttcgccatct ccagatcgcg ctgagctttg
     7321 acgagccaac cccgggcgag ttcctcttta ggcggcatag acgattctcc cgcgcgtctt
     7381 gacctcgcga tgaaagctcc cgatcacgcc tgagtagcgc tccagtttct cggccgtgat
     7441 gaaatgtagt tcgatcggca gacccaagcc gaacagcgcc ccatagccgc gccgtgctac
     7501 atccatgatg gagtcgatgt ggtcaagcac gatcagcaaa tcgatgtcgc tgtcgcgatg
     7561 cggcgtaccg taggcatgcg agccgaagaa atagatggcg cgcggcgtca gagcggccac
     7621 caagcgcctg atgatctcgg caagcatgtc ttgcgagaac tcgggcatca ccccaggtgc
     7681 tgcatcgccg tacccatccg gcgtttgccg agttcccggc cgctcacctt tcatttcgtc
     7741 tccttctgcc cctccccctt aagcgccgcc agttcggccc gcaatgcgct catctgctgg
     7801 ctttcgcggg ccgcctcgac ctgttcttca gcggcgatca tgcggtcgat ctcctcgtcg
     7861 ctgatcgcct cagtcgtccc cgcggccgat acgtccgccg cggccccttg ctcgatggca
     7921 tcgaggaaca tctgcatgcg ctcctccatg ctctgcgact gcatcagcgc ccgctcccag
     7981 tccatctgcg tcttggccag gtccgtatcg ccgtacatgc ggctgacctc gcgggccatc
     8041 aggctcatgg cttcggcgaa gtcggccgag gcttcagcct gccgtttgat caacagcgcg
     8101 tttttcaccg ccaggagctg ccgctcgagc aacttcttga tggtggccgt ctttttcagc
     8161 gaactccgaa tgaactgata ttgcgcctga tcgccgatct gcttggcctg ccgggcattt
     8221 ttgacaaatt catcggtgaa cttgttctgc tcggcaatgg acttctcgat tcgccgtata
     8281 ccttggcgaa tacgcatgtc gcgctcgagc cggcgttctt ctttggactt aaatagaccc
     8341 atgaaccact ccagttgcgg tgatagggtg ataaggtgat agagtgataa aggttgcgtg
     8401 tcgcagccgt ctggcggacg tgagttcagc attcatggaa cgcgctcccc ctgttgatcc
     8461 tcacacgcga ggtacttcgg accgggcagt gtcttacgag taccccgcaa gtgaataatg
     8521 tacgcctgaa gctgccgggc gcagagctcg gctttgtcta tcaaccactc ataaacgtgc
     8581 tggttgatca atccgacgtc ataagcagtg ataatttgac tccgcaattc ggctgcagaa
     8641 cccttggcgg tgtagcagta ctcagtctgt tgtttgcgcg tgcctctctc aaaaccctct
     8701 gcgatgttgc tgccgatgct caccgccgca cgcttcattt ggctagccag cgcgaagtct
     8761 cctcgcagct tttggttctc gctggcccgg tacacaccgc gcgtcaaatc ccgtgcagtc
     8821 tgccaaaccc gcagatcctc aaaccgcctc gccgccacag cgcgctccct ttatcactct
     8881 atcactttat caccttatca cttcacgtgt ctgcagcttt ctcccgctcg cgcacgcggc
     8941 gttccgcctc gtcgaacgcc gtccccgtat ccttgatcag ccccgcgtcc atgtcattcc
     9001 agatcttcag cagctcctgg gcggcgggtg agatgccggc cgtcgattcg tcggcttcgg
     9061 cgcccatgcg caggatctcg tccagacgct cctggtacat ctcactggtg atccgatcgt
     9121 tctcgatcag ccgctcaatg gccgccaggt cgctttcgcg gatcagcggc cgaacgccag
     9181 ccgcgccgcc gaacatctgg ccgctctcgc gcaacatgcg gaggcgcgag actgtcagtg
     9241 cctccttgct gcggatgttg agatgccgcg cgatcatcat ttgctcggtg tgcagcaggt
     9301 cgtattgctg ggcgaatgtg cggcggagtt cgggcgtctt ttccttcgcg ccctgggcga
     9361 tgattttctg cttatctttg gcgagctgcc cgacgcgggc ttgcatgcgg ttgcgctcgt
     9421 tctgtaacag catttcctgg gcgcgcagct cggctacgtt catcgtcgcc agcgtcttgc
     9481 gtttggtcgt gagccattcg aagaagttca ttgaagctct caactaccag ttnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcttggc cgccgcctgg gcctcggcta
     9661 cctgggcgaa ctcggccaga atggcgtctt cctcgtccgc ctgcagcggc gtctgcgcgc
     9721 ctacttcgat gcccgccgcc aagtccgcat tggccgccaa ctcggccacg atctgctcgg
     9781 cttcggccgc ctcgcgcgtc aggtcttccg ccttcggcag ctccagccgg cggccctgct
     9841 cggccagcgt caggttgtgg acgtgcgtgc cgataatgtc gatctgctgg gtgaagacgt
     9901 tcacctgggc ccggatgcgt cgtaagtcgc gccgcacgcg caccagcttg ccggcgagct
     9961 gcttcttttc ggcctcggtc gcggcggccg cccccttctg aagcgactcg cgctcttcgg
    10021 cttccagtgc gtcgagccgg cggtccagct cctcccgctg ttgttcgagt tggacgcgct
    10081 gcgcggtcat gatgcgcacg cgttcagcgg tgctcggctt gaacccaaac agccgtcgta
    10141 tccgcatcca caagctcata gcgccgacct cgtccgccag cgggctgcgc gccacgtgca
    10201 gctcgccctc gtgcacgacc gtcatcaggc gcgggtcgct ccggcacgcc tgccgaaccg
    10261 ccgcttcggt ctgttccagc ggcagcccga gtttatcgga cagctcggag agcgcaatac
    10321 cccgcgactc cagggcagcg gcctctttgt ccagatgata caacaaacgc cggacatggt
    10381 cgtcctgggt ttcgaattcc actaattgcg cccacgggct atagcgcact gagtctgaca
    10441 tctccacgtc ccacccgccg tcctcgcgcc cgcccaccag caccagcttg ggcaggtcgc
    10501 tatcggtcac catggcccgc gcctcgggcg tgaaacccgt cgcactcgcc agtatgacgg
    10561 ccgtcgggcg ctggctggta ggaaggagtt cgtagcgatc cagcgcatat tgcacttgat
    10621 cgcgcccgat cggccccgga gtttcgccgg ccaccagcgc cgccgtcggc gtcagcacct
    10681 gggcccggac gcgcgccacc gtgtacgaca gcggccagat caactgccgc ccaaattcca
    10741 ggctgaccgc gcgcgtcccc ggtaactcgt tcagcaggtc aagatcgtac atctccaggc
    10801 ggttgagcgt ggcccgtacc gcgtcccgac catccggcat ggactcaacc acgacccgat
    10861 cggctggcag cgacccgtga atcagcttcg tcgcccgggc gcgcaactgc cggtgaaacg
    10921 aatcttcgaa ctggcgttga cggctgcgct catccatagg cgtccagatc gtgttcatgc
    10981 tggaacgacc ggccgcaacg tgcacaatag cgcgcatcgc ccgggttggc gtgcccgcaa
    11041 cgtggctctg ggcaaatcag ccggccgcgg cgcgcggccc ccaattgccg atcgccgccg
    11101 ccaatcccgg ccactgcccg gaacacaaac cccagcgtcc ttacgaccag cgcgacggcc
    11161 accacgatca ggcccaacac gccgagcacg aacagaacgc cgacggtcac gtgaaaggcc
    11221 tcggccagca gcacgatcct ctactcctcc agcggcagcg ccgtcccgca gaaaatacag
    11281 aaccggtccc gggcggcggg atagccccgg caggccggac accgtcgcac acggctagtg
    11341 agttggtggc cgcagtaagt gcagaactgg tcgtcagcag ccaccaggtg attacaggac
    11401 gagcaggccc gcaacgcccc gctggcgtca gcgttccaac ctgacggagg aggcggaggc
    11461 acgataccgc ctcgggcggg ccgcacggcg gccagccgcg catccatatc gttgagcacc
    11521 gcacgggcgc tctcgtaacg cgaatcctgc cgcgcgtaca gccgccgata aacttcatcc
    11581 agcgccgtcg gaacttcagg acgcacgctg ctgggcagct cggcgccggc cggccgctca
    11641 cccgtgagca gctcgaacag gattacgcca attgaaaaca gatcgcttag cggcgacggc
    11701 ttgtaatgcc cgtcgcgcag ttccggggcc atataggcca gcgttccgac cagtttgtca
    11761 tctcgatcca gcgaggccga gtgcgcgatg gagcgcaaag tatcggcgtt gctgacgccc
    11821 aacccaaaat cgccgatctt cacgtccgcc acgcgtacgt ccgtgatggg ccgctcgccc
    11881 agatccagca gcacgtttcc gggctt
//
LOCUS       DAOSYS010000097        11816 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4600_length_11816_cov_5.287476, whole genome shotgun sequence.
ACCESSION   DAOSYS010000097 DAOSYS010000000
VERSION     DAOSYS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4600_length_11816_cov_5.287476"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1549)
                     /locus_tag="PLU99_07685"
     CDS             complement(323..1549)
                     /locus_tag="PLU99_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO16974.1"
                     /translation="MQFLRRARDFLGLRRSMVGLLAMVILVGLGEKLAEDFLPIYLIA
                     LGGGFISVGLLNALDNLLGAAYALPGGYLAQRWGPKRALLAFNLIAMAGFLIVILIPA
                     WPAVLIGAVFFLSWTAISLPATMGLVAQVLPRSKRTMGVAMHSLVRRVPMALGPLLGG
                     LLIQRYGTLAGVRLAFGAALAMAALAALLQQLLIQDQPPAGLPAARVQANPLPILHAM
                     SPALRNLLVADTLIRFCEQIPYAFVVVWAMQTIAHPVRADQFGLLRTVEMTTAALLYI
                     PVAYLADRTVKKPFVLLTFIFFTLFPVVLLLAQSFWTLLGAFALRGLKEFGEATRKAL
                     ILDLAPEDRRTMTFGVYYLVRDSIVALAAFGGAFLWQISPAANFLTAAAFGLAGTLWF
                     ALYGRDLTDAGPQDAR"
     gene            complement(1611..1859)
                     /locus_tag="PLU99_07690"
     CDS             complement(1611..1859)
                     /locus_tag="PLU99_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16975.1"
                     /translation="MSIGVIIAGFAAAAALVLWYWVAATKQVSAELLKEYQRLLREAR
                     EEEAHSDENGVASPPAAPGDSPSEARPSTSPPKSAPQS"
     gene            2183..2752
                     /locus_tag="PLU99_07695"
     CDS             2183..2752
                     /locus_tag="PLU99_07695"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOO16976.1"
                     /translation="MERYEEHQRDISWLRISEVNDLRRLIRDVPDFPKPGVVFKDITP
                     LLADPAGLAWAVELMVQPFRGKRVDLVAGAESRGFIFGTAVARELSAGFVPIRKLGKL
                     PHHTYRHEYVLEYGSDALEIHRDAVKPGQSILLVDDLLATGGTLAACCRLVSDLGGNV
                     TAVAVLIELTELRGRDRLAGYPVFSVLSF"
     gene            2987..3496
                     /locus_tag="PLU99_07700"
     CDS             2987..3496
                     /locus_tag="PLU99_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16977.1"
                     /translation="MKYGSVIASIALGVLALCPSGCTIGMDFFNPNLLRGLGIDPAAV
                     DPPSGTVIVMFVNDTNGVATFHAYEIPNLAHPEQGARNFSVEVQPRGNDNEVLDCPLG
                     MFGLGSLGAGFAVSDETAVDVVIPGGGGTSVPYAGQPLFSPDDFKCGDMILVQLTADG
                     ALLVQLLPS"
     gene            3522..4031
                     /locus_tag="PLU99_07705"
     CDS             3522..4031
                     /locus_tag="PLU99_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16978.1"
                     /translation="MHKLTKTTLAMLVTALLLGGCSPADFLNEQFLASIVGGSAVTLP
                     GNAPTLLVAVENHTNRPAAIQVAYRDSNNALRQYTAGVEPGQRTAKALMCPVSEITVG
                     DLSDPSATGAWIQMAGGGVFIEVEPFGVPLKVEQNYECGDGITFVVQPSGATKSGYQL
                     FAYIQHASE"
     gene            4040..6826
                     /locus_tag="PLU99_07710"
     CDS             4040..6826
                     /locus_tag="PLU99_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HOO16979.1"
                     /translation="MRLSNGRKWAAPAGALTALLLLSGCPGLQNSEYVAGSTGSNINL
                     GNEPSLKIFTPESDLSITQGTPVEVTWLAVGTTSYATVDLFIDNDTDLGNGVLSYPFT
                     GMPLTQMAAQVDTRQLAAGEYRIGLLEKQLGEVVVSAYAPGTLYINSSPTLFFNSPRN
                     NYRYDRSPEIQPSFDVDWTLDPQARVVTTQIFLDPDGSVNGNEVLLRTSSSQTGDRFT
                     FDLPTAQFEPGTYRILAQVTDGRESFAFYAPGSIVLRARLCGAVDLRGLDLATSAIRG
                     AVFEGVNPRDNAGSFVCGTRDLDGDGFDDFLILSQFAKPGYETGVHRTGVGEAYLIYG
                     RPQRFSGKINLNSTGTLFRGDIFAGVPEMADPIRPSRGITSFTMLSDWDLDGLREFAF
                     GLPFTDSYNKYNLPLEPSGYFRTGGVVVACGSVLRPDVGFPGRNVIDLGLIGTEPHKA
                     IKVPVGCPEGFVSAKYSLSSHTGPEGTTLFHEHLSSARRGMALTLGCRFASNDFGDQF
                     GESISSWDFHSLVISAPNRDPSVGVRGAESAPGAGMISIYFNATYSPFFPWTLDNAPP
                     GNEAYNYAPAGNHPAASLLPHGGPYHYVLDDIRGQWSSPGYWVEPDDNPPCVKSAYPM
                     STLTIWSDVPGAHLTHARGIGDMNADGLFDLLVGAPFMNDGAGACFIVLGRVADLLEN
                     QTLNVRELSMPMDSSSPHERRILDGIQLVGEPGDRLGQEQDDAGDFNNDGLADVVIGS
                     PELDNRRGGAGVFFGSREAINLTQQEIRFSELPALGLGVIFLGREEGDLAGARVTTAG
                     DVDGDGNSDILIAAPEASVRMDIDQDGTIEIDRTRCGVVYLIYGSPDLKGTISLADIG
                     TERLPGVMFIGRNSGDELGAGIGEMGDRSRGMAMAGDVDGDGHGDLLLGSVPSSPRDR
                     ARAGEAYLIYGVGD"
     gene            6842..7651
                     /locus_tag="PLU99_07715"
     CDS             6842..7651
                     /locus_tag="PLU99_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16980.1"
                     /translation="MRTFFVGLTVLLVCAITGCGGAPTPANPFMTLAERSGAGASQNR
                     PSGGSSGQQAQSTAFRASMNVTFNNNSGYELDTSFLAWVAPGGVRTAEQQEELLNSNY
                     IQITEELRLGSAVTLGAGTYVYNGPGAAHVTPVHLEPSQAGGGTTGATLSSMTIRLVA
                     PDGLLVFSQPPVSCDSVAFTFSQQGEPLPPVPAGYGIWNGATNTGGLKTLAQVSAYQC
                     DPFKPGLFLYRSGGPRQSNEFLESDDITFEFRLFPDENGYCANVTIGASGS"
     gene            7699..9087
                     /locus_tag="PLU99_07720"
     CDS             7699..9087
                     /locus_tag="PLU99_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO16981.1"
                     /translation="MVRNATAAALVALVGVGLALGQGNEPPIVIGYRIYVNGVFATDH
                     NNPVPVRALEQDRIRVEIDVLIPQVDEEEESEPEEVPEFFYEKISEWMPYPYGSPEGP
                     PINLDTALNEFSPLVPYRLEHPEYSLAISFYLPEINGANQARLRGLVDYDVWYEVRIR
                     IWNKETAGEADPVHGFSFSLYVNEDPSLKPPVPPVFADAGSDVLAALGQTVNLDARRT
                     FDAYNVGFDPMDPNVYEKDLVIYTWQWLSGPVRNAASEPVKPDPDRQPWLAQVTPDTL
                     GTYEYRVFVRTASNPTPRSDTVKVTVVPELPPNHAPRAVIDGPSEPVVVGQVVTLDAS
                     SSSDPDGDALTYRWRQTDEVGGVLPAEVLATTFQPLSGLQQAQSQWQAIAPGTYYFIL
                     LVNDGQAGSSARTTVTVVPTANAGESVTRDATAEQVAAPLAGVPTAGCGAGLTPLVVV
                     PFVLLLLRGRMR"
     gene            complement(9269..10450)
                     /locus_tag="PLU99_07725"
     CDS             complement(9269..10450)
                     /locus_tag="PLU99_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16982.1"
                     /translation="MFALAMRPRRGGHAVQVLGADIADRAAVGSACAEDQGNRQDAKN
                     AENAKYKGERPESGERRRKARLAELAEQVRRICRRGAVDLPPCPSGLAELDEALRGGF
                     ARGAIHELIAAGEGAAARSIALLTAARAAGEHKSILFIDTAGDFYPPGAAQLGVPLER
                     LVIVRPGSRVDALWACEQALRCTAVAAVITCLPRALETQISRRLQLAAEAGRNVGLLV
                     YTQKAAASGQKAAAFGIRNGSSAIQTFAATRLSLEPLEKRMEPPEKRIGSRAGSCRAG
                     ISGSDLIREEFGARFKTARHLENGTPFTGGSPFTSTGATRPGSARRLRVRILKLREGQ
                     PVEPFVLEIPEPFELDIPGPNPMLLGPPELSSSPECLERGSDLAAEHPEHNSRWWAQA
                     V"
     gene            10700..11671
                     /gene="murQ"
                     /locus_tag="PLU99_07730"
     CDS             10700..11671
                     /gene="murQ"
                     /locus_tag="PLU99_07730"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOO16983.1"
                     /translation="MPQPEPPSMHDRGHLLTELRNPRSAKIDRASIAEAFDLMNAEDA
                     TVAAAVAAARSEICRATELVVAAFESGGRLIYVGAGTSGRLGVLDAAECPPTFLSDPA
                     MVQGFIAGGWDALRRSIEGAEDHPADGAALIDDLNIGPHDVVMGITTGGTTPYVHGAL
                     ERARERGAKTIFFACVARDHVPDQADVSIRVLVGPEVITGSTRLKAGTATKMVLNMIT
                     TISMVRIGKVYQNLMVDVNTRANRKLVDRGTRIIQTITGLERPAAAALLQQAGGRVKV
                     ALVMHALRLTPEQAWQLLQQNGGHLARVLKNATTETQRHREDTEKEE"
BASE COUNT         2181 a         3681 c         3812 g         2142 t
ORIGIN      
        1 atcgtgcatg tggcggatcc actcgtgcat gttgtcgccg catccgaatt tgctgaagct
       61 catgcgacac cgcgctttct gggccgggag ttggcccggg ctcgcgcagc gggcgttggt
      121 tctggcgtta cggggctgac gcacccggaa ctggccttac gggctcgaag ccggaccaat
      181 gcccgcggtc acggcctgat tgtaccgcat ttcgcgtttg cgtgggccac gtccaatgac
      241 aggccgggcg cggcttctgc ggcatgcttt tgacttccaa gcctgacttc cgggccggcc
      301 acggctgaac atggcgggcg gctcagcggg cgtcttgcgg cccggcgtcg gtcaggtcgc
      361 gtccatacag ggcgaaccag agggtgccgg ccaggccgaa cgcggcggcg gtcagaaagt
      421 tagcggccgg gctgatctgc cataggaaag cgccgccgaa ggccgccagc gcgacgatgg
      481 aatcgcgcac gaggtagtag acgccgaagg tcatggtccg acggtcctcg ggtgccaagt
      541 caaggatgag tgccttgcgg gtggcctcgc cgaactcctt caggccgcgc agcgcgaacg
      601 cacccagcag ggtccagaat gattgtgcca gcagcagcac cacgggaaac agggtgaaga
      661 agatgaaagt cagcaggacg aacggctttt tgacggtgcg gtcggccaag tacgcgacgg
      721 gaatgtagag cagggcggcg gtggtcatct cgacggtgcg gagcagaccg aactggtcgg
      781 cgcggacggg gtgggcgatg gtctgcatgg cccagacgac gacgaaagcg taaggaatct
      841 gctcgcagaa gcggatgagc gtatcggcga cgagcaggtt gcgcagggcc gggctcatgg
      901 cgtgcaggat gggcagcgga ttggcctgca ccctggcggc cggcagtccg gccggcggct
      961 ggtcctggat gaggagctgt tgcagtagtg cagccagggc ggccatggcc agggcggcgc
     1021 cgaacgccag gcgcacgccg gcgagagtgc cgtagcgctg gatgagcagc ccgccgagca
     1081 gcggcccgag ggccatgggt acgcgtcgga cgagcgagtg catggcgacg cccatggtgc
     1141 gtttggagcg gggcaggacc tgggcgacga ggcccatggt ggccggcagt gagatggcgg
     1201 tccaggagag gaagaagacg gcgccgatga ggacggcggg ccaggcgggt atgaggatga
     1261 cgatgaggaa tccggccatg gcgatgaggt tgaaggccag cagggcgcgt ttggggcccc
     1321 agcgttgggc gaggtatcct ccgggcagtg cgtaagcggc cccgagcagg ttgtcgagtg
     1381 cgttgaggag tccgacggag atgaacccgc cgccgagcgc gatgaggtag atgggtagga
     1441 agtcttcggc cagcttctcg cccagaccga cgagtatgac catggccagc aacccgacca
     1501 tgctgcgtcg caggccgaga aaatcacgcg cgcgtcgaag gaattgcatt taaggcgatt
     1561 gtagttggtg cggcggcgtg cggccgtccg gacactaggg cgcgttacgc ttaggattgc
     1621 ggggcgctct tgggcggcga agtggacggg cgtgcttccg aaggcgaatc gccgggcgcg
     1681 gccggcggcg aagccacgcc gttctcgtca gaatgtgctt cttcctcacg ggcttcgcgt
     1741 aacaggcgtt gatattcttt cagcagttca gcgctgacct gcttggtagc cgccacccag
     1801 taccacagca ccagcgccgc cgccgctgcg aaccccgcga tgatgacgcc gattgacacg
     1861 tcgctcttgt cccggcgtgc gccgcgtccg tgccaggcgc tctttctact ccgacccgcg
     1921 cgccgtcggc tggcgcgcac gacccggccg agacgtgcgg cccgggcgta gggcggcggt
     1981 ccgtcgcctg ccccggaggc ttatcggcaa ccgcccccag caggtgaaat cgcgccgggc
     2041 ggcgttgcgg ggcaagcgcc gcgctactag gatacgcccg ccccgaaaag ctgcgcctgc
     2101 ctcgcccggt tgccggatgc tgggggtcct aggcgatggg gggcgggcag gaattgagac
     2161 ccaggagcga cggttcctcg ccatggagcg ttacgaagaa caccaacgcg atatcagttg
     2221 gttgcggatc tcggaggtga acgacctgcg ccgcttgatc cgcgacgtgc cggattttcc
     2281 caagccgggc gtggtgttca aggatattac gcccttgttg gcggaccccg cgggcttggc
     2341 gtgggcggtg gagctgatgg ttcagccctt ccgcggcaag cgggtcgacc tggtcgccgg
     2401 cgccgaatcg cgcggattca ttttcggaac ggcggtggcg cgggagttat cggccggctt
     2461 tgtcccgatc cgtaaactgg gcaaactgcc gcaccatacg tatcgccacg agtacgtcct
     2521 ggaatatggg tccgatgcgc tggagattca tcgcgacgcc gtcaaacccg gacagtccat
     2581 tctgttggtg gacgacctgc tcgccaccgg gggaactctc gcagcttgtt gccggctggt
     2641 gagcgatctg ggaggcaacg taacggccgt ggcagtgtta attgaactta cggagctgcg
     2701 aggccgggat cgactggcag gttatccggt ctttagcgtg ttgagctttt gaccggtcgc
     2761 aggcgcggcc ctaagttggt ggctgtgtga gacttgcgat taacagagcg gccaggcgga
     2821 gccccacagt ccgggacacc ctggagattt tggccgcccg gcacaatgga attgcgcagg
     2881 aatttccgag tgcggaaagt attgcgctgc ccgacggccc aagcgctttt ggctgatgga
     2941 tggcgaagtc cgcggctgtg cgccgggctt tgcgtggagg tgttggatga aatacggcag
     3001 cgtcatcgcg agcatagcgc tcggtgtgct ggcgctgtgc ccttccgggt gcacaatcgg
     3061 tatggacttc ttcaacccca accttctcag gggactgggg attgaccctg ccgcggtgga
     3121 cccgccgagc ggcacggtga tcgtgatgtt cgtgaacgac accaacgggg tggcgacgtt
     3181 ccatgcttac gaaattccga atctcgctca ccccgagcaa ggcgcacgca atttttctgt
     3241 cgaagtgcag ccccgcggga acgacaacga agtgcttgat tgcccgttgg gcatgttcgg
     3301 gcttggcagc ctcggcgcag gtttcgcggt gtccgatgag accgcggttg acgtggtgat
     3361 tcccggagga gggggcacct ccgttcccta tgctggccag ccgcttttct cgccggacga
     3421 tttcaagtgc ggcgacatga tcctggtcca gctcactgcg gatggcgcct tgctggtcca
     3481 attattgccg agttgagcgc gatcggagga gcacgccaca aatgcacaag ctgactaaaa
     3541 caaccctggc catgctggtc acagcactcc tgctcggcgg ttgttcgccg gcggactttt
     3601 tgaacgagca gttcctggca tccattgtgg gcggttcggc cgtcacgctg ccaggcaatg
     3661 cgccgacgct tctggtggcc gtcgaaaatc acacgaatcg cccggcggcc attcaggtgg
     3721 cataccgcga ttcgaataac gccctgcgac agtacaccgc tggagttgaa ccaggacaac
     3781 gcacggccaa ggccctgatg tgcccggttt cagagataac ggtaggcgat ttgtccgacc
     3841 cctcggctac gggggcgtgg atacaaatgg ccggcggcgg cgtgtttatc gaggttgagc
     3901 cgttcggcgt gccgctgaag gttgagcaaa actacgagtg cggggatggg attacgtttg
     3961 tggtgcagcc ctcgggcgcc acgaaatcgg ggtaccagct attcgcctac atccagcatg
     4021 cgagtgagtg aggatgagaa tgcgtttatc caacgggcga aaatgggccg caccagccgg
     4081 cgcattgacg gcattattgc ttttgtcagg ctgtccgggc ctgcaaaact cggaatacgt
     4141 tgccggctcg accgggagca acatcaacct cggcaatgag ccttcgctca agatcttcac
     4201 gccggaatcc gacctgtcga tcacgcaagg cacaccggtt gaggtaacgt ggctggcggt
     4261 cggtactacc agctatgcca cggttgacct gttcattgac aacgataccg acctggggaa
     4321 cggcgttttg tcgtacccgt tcacgggcat gccgctgacg cagatggcgg cgcaagttga
     4381 tacgcggcaa ttggcggcgg gcgagtatcg catcgggctg cttgaaaaac agctcggaga
     4441 agtggtagtc tcggcgtacg cccccgggac actatacatt aacagctcgc ccacgctgtt
     4501 cttcaactcc ccgcggaaca actaccgcta tgatcgttcg ccggaaattc agccgtcctt
     4561 cgacgtggat tggacgctcg acccccaggc ccgcgtggtg accacgcaga tcttcctgga
     4621 cccggatggc tcggtcaacg gcaacgaggt gctgctgcga accagttcgt cgcagaccgg
     4681 agaccgcttc accttcgacc tgcccacggc ccagttcgag cccggtactt accgcatact
     4741 ggcccaggtt acggacggga gggaatcgtt cgccttctat gcgccgggca gcatcgtgct
     4801 gcgggcgcgc ctgtgcgggg cagtcgacct gcgtggtctg gacctggcga ccagtgccat
     4861 acgcggggcc gtgttcgagg gcgtcaatcc gcgcgacaac gccggcagct tcgtgtgcgg
     4921 cacgcgcgat ctggacggcg acggctttga tgatttcctg attctctcgc agttcgccaa
     4981 acccggctac gaaaccggcg tacaccgcac gggcgtcggc gaagcttacc tgatttacgg
     5041 ccgcccgcag cgtttcagcg gcaagatcaa cctgaactcc accggcacct tgttcagagg
     5101 cgatattttc gccggcgtgc cggaaatggc ggaccccatc cgtcccagcc gcggcatcac
     5161 gagcttcacc atgctgagcg attgggatct ggatggtttg cgggagttcg cgttcgggtt
     5221 gccattcacg gattcttaca acaagtacaa cctgccactc gagccgtccg ggtatttccg
     5281 caccggcggc gtggtcgtag cttgcggttc ggtattgcgg ccggatgttg gattcccggg
     5341 gcgcaacgtc atcgacttgg gtttgatcgg aactgaacct cacaaggcta ttaaagtacc
     5401 tgttggctgc ccggaaggct tcgtaagtgc gaagtactca ctatcctcgc ataccggtcc
     5461 ggaaggtacg acgttattcc acgagcacct gagcagcgcg cggcggggga tggcactgac
     5521 gctggggtgc cgcttcgctt cgaacgactt cggagatcag ttcggtgaat cgatatcgag
     5581 ctgggatttc cactctctcg tcatttcggc cccgaaccgt gatccgtcag taggggtgcg
     5641 gggggccgaa tcggcgcccg gtgcaggcat gatcagcata tatttcaatg ccacttattc
     5701 cccgttcttc ccgtggacgc tggacaacgc ccctccggga aacgaggcat acaactatgc
     5761 cccggcggga aaccaccctg cggcgagctt gttgccgcac ggcggaccat accattacgt
     5821 gctggatgac atccgcggcc aatggagttc accgggttat tgggtcgaac cagatgacaa
     5881 cccgccctgc gtcaagtcag cgtatcccat gagcaccctg acgatctgga gcgacgtgcc
     5941 cggagcgcac ttaacgcacg cgcgcggcat cggggatatg aacgccgatg gcttgttcga
     6001 cctgctggtc ggggcgcctt tcatgaacga tggcgccggc gcgtgcttca tcgtcctggg
     6061 ccgggtagcc gatctgctgg agaaccagac actcaacgtg cgcgaattgt cgatgccgat
     6121 ggactcgtcc agcccgcatg agcgtcgtat tctcgacggc attcaactcg tgggcgagcc
     6181 tggagatcgc ttgggccagg agcaggacga cgccggggat ttcaacaacg acggtctggc
     6241 ggacgtggta atcgggtcgc cggaactgga caaccgccgc ggcggggccg gggtgttctt
     6301 tgggtcgcgg gaagcgatca acctgacgca gcaggagatt cggttctccg aattgcccgc
     6361 gctgggcctg ggagtgatct tcctggggcg cgaggaaggc gatctggcag gcgcgcgcgt
     6421 cacaacggcg ggcgacgtgg acggcgacgg caatagcgac attctgattg ccgctcccga
     6481 agcttcggta cgcatggaca tcgaccagga cggcacgatc gagatcgatc gcacgcgctg
     6541 cggcgtggtg tacctgatct atggttcgcc cgacctgaag ggcaccatca gtctcgcgga
     6601 tatcggcacc gagcgtctgc ccggcgtcat gttcatcggg cgaaatagcg gagacgaact
     6661 cggcgctgga atcggtgaaa tgggcgaccg cagccgcgga atggcgatgg ccggtgacgt
     6721 cgatggcgac ggtcacggcg acctgcttct gggttcggtc cccagctcgc cgcgcgatcg
     6781 cgcgcgagct ggcgaagctt acttgatcta tggcgtgggc gattgatcgc gcgaggtcaa
     6841 catgagaacg ttttttgtcg ggcttacggt tttgctggtc tgtgcgataa ccggctgtgg
     6901 cggggcgccc acgccggcga accccttcat gacgctggcc gaaaggagcg gcgctggggc
     6961 gagtcaaaac cggccgagcg ggggtagcag cggccagcag gcccagtcga cggcctttcg
     7021 cgcctcgatg aacgtgactt tcaacaacaa cagcggctac gaattagata cctcgtttct
     7081 ggcctgggtc gccccgggcg gcgtgcgcac ggctgagcaa caggaagaac tgctgaacag
     7141 caactacatt cagattacgg aagagctgcg gctgggctcg gcagtcacgc tgggagcggg
     7201 aacttacgtc tacaacggcc ccggcgcggc gcacgtaacc ccggtacacc tggagccctc
     7261 acaagcaggc ggcggcacga ccggggcgac gctgtcctcc atgaccattc gactggttgc
     7321 accggacggg ctgctggttt tctcgcagcc gccggtttcg tgtgacagcg tcgcgtttac
     7381 gttttcgcag caaggcgaac cgctgccgcc ggtgcctgca ggctacggga tctggaacgg
     7441 cgcaacgaac actggcggtc tgaagactct ggcccaagtg agcgcctatc agtgcgaccc
     7501 cttcaagccg ggactgttcc tgtaccgctc gggcggacca agacagtcga atgagttcct
     7561 ggaaagcgac gacatcacct ttgagttccg cctgtttcct gacgaaaacg ggtattgcgc
     7621 aaacgtcacc atcggagcga gcggctctta gtccacgact cgctggcctg aggtgctgag
     7681 tattgggaga tcagtatcat ggtgcgaaat gcaaccgccg ctgctttggt cgctctcgtc
     7741 ggcgtgggat tggcactggg gcagggaaat gagccgccca tagtcatcgg ttaccgcatt
     7801 tacgtaaacg gggttttcgc aacggatcat aacaacccgg ttcccgtgcg ggccttggaa
     7861 caggacagga tccgcgtcga aatcgacgtg ttgatcccac aggtggacga ggaggaagaa
     7921 tcagagccgg aggaggtacc cgagtttttc tacgagaaga ttagcgaatg gatgccctat
     7981 ccgtatggct ctcccgaggg ccctccgata aatctagata ccgccttgaa tgaattctcg
     8041 ccattggtac cctataggct ggagcatcca gagtattctc tggcgatttc gttctacctg
     8101 cccgagatca acggtgccaa ccaggcgcgg ctgcgcggct tggtcgatta tgacgtctgg
     8161 tacgaggtcc gaattcggat ttggaataaa gaaacggccg gtgaagccga tccggttcac
     8221 ggcttctctt tctcgctgta cgtgaatgag gaccccagcc tgaagccgcc cgtgccgcca
     8281 gtgtttgcgg atgccgggag tgacgtgctg gccgccttgg gccagacggt aaacctggac
     8341 gcccggcgga ctttcgacgc ctataacgtc ggtttcgatc caatggatcc gaacgtctac
     8401 gagaaggacc tagtaatcta cacgtggcag tggctctcag gccccgtgcg caacgcggcc
     8461 tcggaaccgg tcaaacccga cccagaccgg cagccgtggc tggctcaggt gaccccggac
     8521 accctcggga cttacgagta tcgcgtcttt gtccgcaccg cctccaatcc gaccccacgc
     8581 agcgacaccg tgaaggtcac ggttgtcccg gaacttccac ccaaccacgc gccgcgcgcg
     8641 gtgattgacg ggcccagtga accggtcgtc gtcggccagg tcgtcacgct ggatgcatct
     8701 tcctccagcg atccagacgg cgatgcgctg acttatcgct ggcgccagac ggacgaagtg
     8761 ggcggagtgc tcccggcgga ggtcttggcg acgactttcc agccgctcag tggtctacag
     8821 caggcgcagt cgcagtggca agcgatcgcc cctggcacct attacttcat acttctggtg
     8881 aacgacgggc aagccggtag ttccgcccgc accaccgtca ccgtagtgcc cacggccaac
     8941 gcgggcgaat cagtgacgcg cgacgcaacc gcggaacagg tcgcggcccc cttggcaggc
     9001 gttccaaccg ccggctgcgg cgcgggcctt acgccgctgg tcgtagtccc attcgtcctg
     9061 ctgctgctcc gcggccgcat gcgataggtc agaaggcaga agccggcagt cagaaagcag
     9121 aagtaaaaga gtggcacgga gtcgtaccgg tgtccggtct cgactcggtg ccacttttgc
     9181 ttgcctgccc cggaacccgg catagcagac acagcgtgtg cccgctgctt caggatgccg
     9241 cggggcggcc gaatggcgcg cggcgacact aaacagcttg tgcccaccag cgactgttat
     9301 gttcgggatg ttcggctgct aagtcgctgc cccgctccaa gcattccggg gagcttgaca
     9361 gctccggcgg tcccagcagc attgggttcg gtcctggaat gtccagctca aacggttctg
     9421 gaatctccag tacaaacggt tccaccggct gcccttcgcg cagcttcaat atcctgaccc
     9481 gcaaccgccg agcagagccg gggcgtgtcg ccccggtaga tgtaaacggc gacccgcctg
     9541 taaacggcgt cccattctca agatgccgcg ctgttttgaa ccgtgctccg aactcctcgc
     9601 gtatgagatc cgatccgctg ataccggctc gacacgagcc ggctctgcta cctatccgct
     9661 tttccggcgg ctccatccgc ttttccagcg gttccaggct cagccgggtc gccgcgaacg
     9721 tctgaatggc gcttgagccg ttgcgtatcc cgaaagccgc cgccttttgc ccggaagccg
     9781 ccgccttttg agtgtaaacc agcagcccca cgttccgccc ggcctctgcc gccagttgta
     9841 gccgccgcga aatctgcgtc tccagcgctc tcggcaaaca cgtgatcacc gcagccacag
     9901 ccgtgcagcg caaagcctgt tcgcaagccc acaacgcatc aacgcgactg cccggccgca
     9961 cgatcaccag ccgctccaac ggcacaccaa gctgagccgc tcccggcgga taaaaatcgc
    10021 cggcggtgtc aatgaaaaga attgatttat gctcgccggc cgcgcgagcg gcggtcagca
    10081 gcgcgatcga ccgtgcggcc gccccctcac ccgcggcaat taattcatga atggccccgc
    10141 gagcaaatcc gccccgcagc gcctcatcca gctcagccag gccgctcggg cacggcggca
    10201 aatccaccgc cccccgccgg cagatgcgcc gcacctgctc cgccaactcc gccaggcgcg
    10261 cctttcgccg gcgctcgcca gactccgggc gctcgccttt gtacttggcg ttctcggcgt
    10321 tcttggcgtc ttggcggttg ccttggtctt ccgcgcaggc agacccgacc gccgcccgat
    10381 cggcgatatc tgcgcccaac acctgcacgg cgtgcccacc acgccgcggg cgcattgcca
    10441 gagcaaacat ttccgttccc actgatacgg gcgagccggt cgcgtcccat gctcgcttac
    10501 gcgacttgcc ttcaccgccc gccttccgtt cttacctaat aataccctaa caaaacgccg
    10561 gcgtcaaaca aagttgcttc attccgccag tacctgcggt tatcatgccg cgaccggcca
    10621 cccagcgcgc cgcaggcggc cggcaacagc gagcgctcgc cgccagcggc ccgcattaaa
    10681 aggaattaag ccgcccccaa tgccgcaacc cgaaccgcca tcgatgcacg accgcggaca
    10741 cctcctgacc gagctgcgca acccgcgctc ggccaaaatc gatcgcgcct ccatcgccga
    10801 ggccttcgat ctgatgaacg cggaggatgc caccgtcgca gccgccgtcg ccgcggcgcg
    10861 ctccgaaatc tgccgcgcca ccgaactggt ggtcgccgcc ttcgaaagcg gcggacgcct
    10921 gatctacgtc ggcgccggaa ccagcgggcg gctgggcgtg ctcgatgccg ccgaatgccc
    10981 gccgaccttt ctctccgacc ccgccatggt gcaggggttc atcgccggcg gctgggacgc
    11041 cttgcgcaga tcaatcgaag gcgccgaaga ccaccccgca gacggcgccg cgctgatcga
    11101 cgacctgaac atcggcccgc acgacgtggt catgggtatc accaccggcg gaacaacgcc
    11161 ttatgtacac ggcgcactcg aacgggcccg cgagcgcggc gccaaaacaa ttttcttcgc
    11221 ctgcgtcgcg cgcgaccatg tacccgacca ggccgacgtc tcgattcgcg ttctcgttgg
    11281 cccggaggtg atcaccggca gcacgcgcct caaggccggc accgccacca aaatggtgct
    11341 gaacatgatc accaccatca gcatggtgcg catcggcaag gtctaccaga acctgatggt
    11401 ggacgttaac acccgcgcca accgcaaact ggtcgaccgc ggcacgcgca tcatccagac
    11461 catcaccggc ctcgagcgcc cggccgcggc cgccttgctg caacaagccg gcgggcgcgt
    11521 aaaagtggcg ctcgtcatgc acgcactgcg cctaacccca gagcaggcct ggcaactact
    11581 gcaacaaaat ggcggccatc ttgcgcgtgt actgaagaat gcgaccacag agacacaaag
    11641 acatagagag gacacggaga aggaggagta gttcgtcatg ctggcgccat tcgaccgggg
    11701 ccgcctctct gtggttgcct gtctcttcgg gaagagcaag ggatgctagc cgcgttcagt
    11761 gcccagttca cctggattga ctggctggtg gtcgtcgcct acctcgggtt cactac
//
LOCUS       DAOSYS010000098        11804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4607_length_11804_cov_5.382245, whole genome shotgun sequence.
ACCESSION   DAOSYS010000098 DAOSYS010000000
VERSION     DAOSYS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_11804_cov_5.382245"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(669..1286)
                     /locus_tag="PLU99_07735"
     CDS             complement(669..1286)
                     /locus_tag="PLU99_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16984.1"
                     /translation="MLVTLDGRLLQRSFPPGCTLQDVIDQARALLDPQRLVVGVAVDG
                     QDYLEQQLEVQLSQPLLPDVQVDLESADRSTVAVAALRAMAEEMERAADTLAEIARQL
                     NAGQSAEGVRRIGDFLKTWQNCRNVVLQSGALVGRNLLQVSIESGTVAECLADLVERL
                     RDLRDALEARDLVLLADLMQYEIPELCKKWKGLLLSLAESVACGA"
     gene            1658..1969
                     /locus_tag="PLU99_07740"
     CDS             1658..1969
                     /locus_tag="PLU99_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HOO16985.1"
                     /translation="MSARRPAWLENWLARHRSRLSFWLHMLGIPLAVAGCLLALWQLV
                     QWRWDLWWRPVALIAAGYALQIIGHRHEGNDVGEWALIKRLLGRPYVAISPKYRSEQR
                     Q"
     gene            2227..3834
                     /locus_tag="PLU99_07745"
     CDS             2227..3834
                     /locus_tag="PLU99_07745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO16986.1"
                     /translation="MRLIVLQQGAMIEDIVCGEEAIYVGAHEDCRIRLQDPRIAPRQA
                     VIYPQGSGWTVEQLAPECAIHVNDMSLAGKSTLKTGDEIRLADYVLRAYPEHVEQPGL
                     ELGTSRAQLERFIASRLPPGTVIKKPTEPLNIAPEQVMKIAEFTQRVSQCEQVTELMD
                     VALQALLVNFAAQRVWMGVRRMTYGAMEYVEGRLLTGQPTDLPEMADCLKPRVLDRIQ
                     YLLVPIFNREERSSVMAGPLRGGDGALGMIYLDSGGSGRMYEDKDMDFFVLFSTVIAA
                     QLDAIFKHIAASRAALVAGEVGVAHVIQARLTPRKLPQWEKLQFGAFRETGRERASDI
                     YDLLKLNDGTANFMVAHTAVKGALPSLLMAEAQATFRLAALHKDPPHVFLRSLNWVLY
                     DGEDDHPLNCFVGTIDPQTGQIRYALAGEARAFIIGNRGEERRLGTLEPTPPLGVVRS
                     AAYALLPEQLAPSETLVVFTPGVTTARNRNNEVFGEERFINILCDGFGQLASAMLKEM
                     LTDLRSFTEGGVQPEDITVLLAHFVRD"
     gene            complement(3848..5506)
                     /locus_tag="PLU99_07750"
     CDS             complement(3848..5506)
                     /locus_tag="PLU99_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO16987.1"
                     /translation="MASGNGQIPRVSSPPDAGYSRAPLLVCTLLVGATVIVNQSAVHW
                     RENVVDSHLFAWHGWCVAQGERPYIDIWDNKPPGIWWLNAAGFWVCGEGFAGEVLISA
                     TALAVALIAFTATARAAYQPSLLLIAALTGCALLPHSMYEGGANRTETFVLACESAAV
                     CGYVCWLRRRRWPWLIAAGLAAGAAPLFKQSGLAAAAGIGVHLTISLLTAHYRRARGN
                     ASPGQCRPSAKAVLLFAAGCGIAPALALAALAAQGAVVEAWHAVAVFNRVYFEANDAT
                     YLRVDRALHVFGPALVPLGGVSVAAVIGPVWRLTAALVERRRRTVPASASLANSSNAP
                     SRTYVELFWLWFVFAAYLACVGPGRRGHHFLPALAPLGLLALYPVHLLASSAGLGRRV
                     TAHPSSAAIVVAYLYVLALLVSGTATEIGRCWRSKSHWYSLEYAAEPPWQQQAALIRR
                     YARSDEAIYVWGWSPGTYRYAYRRCAARFATFEKVGQLGRRVQFILESALDDLRRNPP
                     RVFVISRPDLAGVMRTPSDEFAQWLSTHYEDRGVTAGMHIMTAR"
     gene            complement(5473..6090)
                     /locus_tag="PLU99_07755"
     CDS             complement(5473..6090)
                     /locus_tag="PLU99_07755"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOO16988.1"
                     /translation="MNELLLATKNPGKLRELQEIAAPLPLLWRSLAEFPHVPDALETG
                     ATFADNARLKALHYSAATGLPTLADDSGLEVDYLGGAPGVHSARYAGPQQDSAANIRK
                     LLAALAGVPPQQRTARFRCSMALARPGEILAESTGLLEGRIADAPRGAGGFGYDPVFV
                     VSELGLTAAELTPQQKHAISHRGQALRAMLPLIEQWLRATGKYRE"
     gene            complement(6083..6955)
                     /locus_tag="PLU99_07760"
     CDS             complement(6083..6955)
                     /locus_tag="PLU99_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16989.1"
                     /translation="MQRNPRSLLPLACVLLSLTGCTAGGAGLGLSPTPGSEVFWAIRC
                     DTRHGADRFRITQQESEALKQVQGLKPQLVQVIHNEHESSVYYGRYRRTVEIGGSTAT
                     YRPDAMADLNLIRSLCMNVGGSDCWPFIYASLEELPSGRSRHPEWDLANAKGYWTLHV
                     AVFYCEGPITNPKYLAEEYCRELREQGVEAYYYHGPTRSSVYVGLFPKEAIQTVSETN
                     PLSGVLTVSNKIVDQRLLKLQEQFPVSYQNGRRVNELVPDPTTGQKRRVPYESFVVQV
                     PSAAKTDRPRAKYE"
     gene            7050..7604
                     /locus_tag="PLU99_07765"
     CDS             7050..7604
                     /locus_tag="PLU99_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16990.1"
                     /translation="MSERPVNRKAERCPRCKYPYHGLPADSACPGCGMQVRTGALVFY
                     GPRRPFAVLDVLYLVLAGWFALALPGNISRQDMILFLCNAVAALLVALLWWSSRRRRY
                     CYVVVDGDRVLYGPGRDAPTELRLHGVWRAEYSALAREVCLRDACGRTIATVPCPWDS
                     GATGHQMCEALNREIERGTSRPDG"
     assembly_gap    7606..7705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8147..8842
                     /locus_tag="PLU99_07770"
     CDS             8147..8842
                     /locus_tag="PLU99_07770"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOO16991.1"
                     /translation="MSFAGVLAGLFMLALLVSAGNFSNTDWLFLGLLGAGLMVLGRLD
                     ARYELLPWLAAAAGVALVIAWRILSDPWNTARFAGVTLGLGTLYALGAYAAMWATRRP
                     GLWAGLSGAAAIAHFLLAYWALPEPPFASSWGVSCVLLAAIYVALSAPVIRRRETAAA
                     YDAALGALLTAATVFVSLAIPIELERAWIAVAWALEIPALALVAGRLRVPVLRSRGRC
                     VVGRRRVSPVGTA"
     gene            9338..10090
                     /locus_tag="PLU99_07775"
     CDS             9338..10090
                     /locus_tag="PLU99_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16992.1"
                     /translation="MRWVRFTLASALLVSVAVFTAVKFGSLKVRQLQNQVNQLQQERQ
                     KLMEYAERLSATRRVAQVDVLRQYHDAAGNPVTALVWQEIGPDGTLGRPVAVEATGTL
                     VYFEALVIKFAQQYVGEGDAERGTSLAMFRRIFGDRQAPESAPDLDRAARPPNTQSSA
                     AAALEDELWAKFWELVDDPQLAAKYGVRVAQCEAPAVPVRPGQVWEVSLDAAGGLNLR
                     LIGTRGEPLSGRGQQTAAAGIQNAAWPRPAAP"
     gene            complement(10092..10994)
                     /locus_tag="PLU99_07780"
     CDS             complement(10092..10994)
                     /locus_tag="PLU99_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16993.1"
                     /translation="MRSFFLVGGLACLAGIIFVKGYLLRPVGAASSSETGTEIRFEYT
                     AYDEEPPPPLPTRPAEQPEVTLAPQPDNSASLPPVDTEPPSESLQPEQDLPAASPTPH
                     ADAGPDRTVWMGDHEITLDGTGSHGTELSYSWRQLSGPVDLLIDNPRAANTVAAGLAQ
                     EWPERDATYEFELTVRDAHGREAVDVVRFTVQAAPSITVDPAARRRLAWREGYLLGHF
                     ESWKTNRTDDAEVFIIRCPSELTFHHLGGMVDFDIAPLECNSGFEYQLTLFYREPESS
                     TFVEFFVDTPERIPAVLQFGVNWE"
     gene            complement(11252..11599)
                     /locus_tag="PLU99_07785"
     CDS             complement(11252..11599)
                     /locus_tag="PLU99_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16994.1"
                     /translation="MNAWLRRAEMNKNLIIVLVLLAVVSGGFWTWKCRSTSAEVKQVV
                     HRFKDYYVTCKNCGTLAEKVPASEVDAMKSDMQGGYLKCPSCGNYTAKFGKNPFKGSE
                     AQGEQEQADDDEP"
BASE COUNT         2044 a         3801 c         3773 g         2086 t
ORIGIN      
        1 cgttgcgttc gagcatcgcg ggccactggt tacgacgacg aggctttggc gctacgcctg
       61 gacaacgcgc cccggctctg ctggttgagc ggacccgccg gcacctcttg atccctcgat
      121 cccttgatcc ctcgatccct cgcgacgttt ggcgctcttg gcgtcttcgc ggttgcctta
      181 gctgaaagcc gatgggtgat agcggaaagc tttccttatc accctaacac tttatcactt
      241 tatcacccaa agcagcgtct ccccgccagt ggcgcgcaac cacgcacaat ccgcggactt
      301 tggctgagcg ttgaccgctg cacgcctgac atccctctag aatcaataga aaacatcgcg
      361 cccgcgcgca gaaaggcacc cactatgcgg cattcgaaat gtccccccta ctcacctgtc
      421 acctatcaac tgtctccgcg cggccgcctt tgtcacctgt tcgcccctca cctatctcct
      481 ctctccgcgc agcggcgcct caggttatca aaatcgagtg cacataatcc aagagtatct
      541 gatcgcctgc ccccaagacg cgatcccaca cggcttttta tcagaccgct tgagcacctg
      601 aggtggcgcc gcgcccagcc gccgccgctc gcgccagaac tattcactat cccccctacc
      661 actatccact atgcgccgca ggccactgac tcggccaggc tgagcagtag tcctttccat
      721 ttcttgcaca attccggtat ttcgtactgc atcagatccg ccagcagcac caggtcgcgc
      781 gcctccaggg cgtcgcgcag gtcacgcagc cgctcgacca ggtcggccag gcactcggcc
      841 actgtgccgc tttcaatgct gacctgcaac aggttgcgcc ccaccaaggc cccgctttgc
      901 aggacgacgt tccggcagtt ctgccaggtc ttcagaaaat caccgatccg ccgcacaccc
      961 tccgcgctct gccccgcatt caactgccgg gcgatttcgg ccaaagtatc ggccgcccgc
     1021 tccatctctt cggccatggc acgcagggcc gccaccgcca ccgtcgaacg gtccgcgctt
     1081 tccaggtcca cctgcacgtc gggcagcaac ggctgcgaca actgcacttc gagctgctgt
     1141 tccaggtaat cctggccgtc cacggccacg ccgacgacca gccgctgcgg gtcgagcagg
     1201 gcgcgggcct ggtcgatcac gtcctgaagc gtgcagccgg gcgggaaact tcgttgcagc
     1261 agtcggccat ccagcgtcac gagcatgatt gtctccgcag ccgggtcaaa cgtgtccgtg
     1321 gaacgaaccg catcggcggt ccgagccggc ccgattcgag cccgccccat gcgagccaac
     1381 cgatccgagc ccgcagcgcc agcaagggca aacgtccgag cggccaccga tccgagccca
     1441 ccgcgccagc aaggacaaaa ctcggctgcc ctcgcgagcg cttcggggcc gcctcctgcg
     1501 cgttctgcgc gctcacctcg tcccgcacgc gctccgggcg cgcaggtcgg ttgtgggcac
     1561 aagcctctgg ttctatcggt ctgtcggcgc gcggcttctc agcgaaaacg cggcgagacg
     1621 ctcgaaaacc gtgcctgccc ggctatgatt gcggcccatg agcgctcgac ggccggcctg
     1681 gctcgaaaac tggctcgcac ggcaccgcag ccgcctttca ttctggctgc acatgctcgg
     1741 catcccgctg gcggtggccg gctgcctgct ggcactttgg caactggtgc agtggcgctg
     1801 ggacttgtgg tggcggccgg tggcgctgat agcggccggc tacgccctgc aaattatcgg
     1861 gcaccggcac gagggcaacg acgtcggcga atgggcgctg attaagcgcc tgctggggcg
     1921 gccatacgta gcgatttcgc cgaaataccg atcagaacaa agacaatagc gctgcccgcc
     1981 cgcacgcgcg ctgccttatc agcggaacgg tgctcgatta gaggagccgt gccgtcgatt
     2041 agaggagccg tgcactcgat tagcggagtc gctcgattgg cggagccctg cgctcggctg
     2101 gccgaccctt gacgaggtgg ctaacatggg cgcgtccgtg cacctgcgag cgccgcggcc
     2161 gagcgatgtt ccaactgact ttgcggccgg gcgtttttcg tgtgggcagg ccggaaagcg
     2221 gaagcgatgc ggctgatagt tctgcaacag ggcgcgatga ttgaggacat cgtctgcggg
     2281 gaggaggcga tctacgtggg tgcgcacgaa gattgccgga ttcgcctgca agatccgcgg
     2341 atcgcgccac gtcaggcggt gatctacccg caaggctcgg gctggacagt tgagcaactg
     2401 gccccggaat gcgcgattca cgtgaacgac atgtcgctgg ctggcaagag cacgctgaag
     2461 accggcgacg agattaggct ggcggattat gtcctgcgtg cctacccgga acacgtggag
     2521 caacccggtt tggagctggg cacgtcgcgg gcgcaattgg agcggtttat cgcgtcgcgc
     2581 ctgccgcccg gcacggtgat aaagaagccc accgagccgc tcaacatcgc gcccgaacag
     2641 gtcatgaaga tcgccgaatt cacgcagcgc gtctcgcaat gcgagcaggt gacggagctg
     2701 atggacgttg cgctgcaagc cctgttggtg aattttgcgg cgcagcgggt ctggatgggc
     2761 gtccggcgga tgacctacgg ggcgatggag tacgttgaag gccggctgtt gacagggcag
     2821 ccgaccgacc tgccggagat ggccgactgc ctgaaaccgc gtgtgctgga ccgcattcag
     2881 tacttgctgg tgccgatctt caaccgcgaa gagcgcagct cggtcatggc ggggccgctg
     2941 cgcggggggg acggcgcgct gggcatgatc tacctggata gcggcggcag cggtcgcatg
     3001 tacgaggaca aggacatgga cttcttcgtc ctgttctcga cggtcatagc cgcccagctt
     3061 gacgcgatct tcaaacacat cgccgccagc cgcgcggcgc tggtggcggg cgaagtgggc
     3121 gtagcgcacg tcatccaggc ccggctaacg ccgcggaagc tgccgcagtg ggagaagctg
     3181 cagtttgggg cgttccgcga gacgggccgc gagcgcgcga gcgacattta tgacctgctg
     3241 aagctgaacg atggcacggc caacttcatg gtggcgcaca cggcggtcaa aggggcgctg
     3301 ccgagtttgt tgatggcgga ggcgcaagcg acgtttcggc tggcggcact tcacaaagac
     3361 ccgccgcatg tatttctgcg ttctttgaat tgggtgctgt acgacggcga ggatgatcac
     3421 ccgctcaact gctttgtggg aacgatcgac ccgcagacgg ggcagattcg gtacgctttg
     3481 gcgggcgagg cgcgggcctt catcatcggc aaccgcggcg aagagcggcg tctgggcact
     3541 ctggagccca ctccgccttt gggcgtggtg cgctcggccg cttatgccct gctgccggag
     3601 cagctcgcgc cgtccgagac gctggtggtt ttcacccccg gcgtgactac ggcgcgtaac
     3661 cgcaacaacg aagtgttcgg cgaagagcgg ttcatcaaca ttctctgcga cggcttcggt
     3721 cagctcgcca gcgccatgtt aaaggaaatg ctgaccgatc tgcggagctt cacggaaggc
     3781 ggcgtgcagc cggaagacat caccgtgctg ctggcgcact tcgtgcgcga ctgaagccgc
     3841 ggcctcttca gcgcgcggtc atgatgtgca tgcccgcggt taccccgcga tcctcataat
     3901 gagtgcttaa ccattgcgcg aattcgtcgg atggcgttct cattacgcca gccagatcgg
     3961 gccgggaaat cacaaatacg cgcggcggat tacggcgcaa gtcatccagg gcggattcca
     4021 ggatgaactg cacccgtctg ccgagctggc cgactttttc aaaggtggcg aagcgcgcgg
     4081 cacagcggcg ataggcatag cggtaggtcc ccgggctcca gccccagacg tagatcgcct
     4141 cgtcggagcg cgcgtagcgg cgaattagcg cggcttgctg ctgccaggga ggttctgcgg
     4201 catactcgag cgagtaccag tgcgatttgc tgcgccagca gcggccgatt tcagtcgcag
     4261 ttcccgaaac caggagtgcc agaacgtaga ggtatgccac gacaattgca gcgctgctgg
     4321 ggtgtgcggt aacgcgtcgc cccaggccgg cacttgaggc cagcagatgt acagggtaca
     4381 gcgccagcaa gcctagtggg gccagcgcgg ggagaaagtg atggccgcgt cgcccaggac
     4441 cgacgcatgc cagatacgcc gcgaacacaa accatagcca gaacaactcg acgtatgttc
     4501 tggacggtgc attagaggaa tttgccaaac tggcggaagc gggcaccgtg cggcgccgcc
     4561 gttctacgag tgccgcagtc aaccgccaga cagggccgat caccgccgcg acagacaccc
     4621 cacccagcgg aaccagggct gggccgaaga catgcaatgc ccggtcgacg cgcaggtatg
     4681 tggcgtcgtt ggcctcaaaa taaaccctgt tgaagaccgc taccgcgtgc caagcttcga
     4741 ccacggcccc ctgcgcagcc agtgccgcca aggctagcgc cggcgctatg ccgcaaccgg
     4801 ccgcgaatag aagaaccgct ttggcgctcg gcctgcactg gccaggcgaa gcgttgcctc
     4861 gcgccctgcg gtagtgcgcc gtcaaaagcg atatagtcag gtgcactccg atcccggccg
     4921 ccgccgccag cccggactgc ttgaatagcg gcgccgctcc ggccgccaga ccggccgcta
     4981 tcagccaagg ccagcgtctg cggcgcagcc agcacacgta gccacagacg gctgcgcttt
     5041 cgcaggccag tacgaaggtt tcagtccggt tggcgccgcc ctcgtacatc gaatgcggta
     5101 acagcgcgca gcccgtcagc gctgcaatca acagaagcga cggctggtaa gcggcccgtg
     5161 cagtcgcggt gaaagcaatc agtgcgacgg ccagtgcagt cgcggagatc aacacctcgc
     5221 cggcgaaacc ttcgccgcag acccagaaac cggccgcatt gagccaccag atgccgggag
     5281 gtttgttgtc ccagatgtca atgtatggcc gttcgccctg cgcgacacac cagccgtgcc
     5341 aggcgaacag gtgtgaatcg accacgtttt cgcgccagtg gacggcggac tgattgacaa
     5401 tgaccgtggc gccgacgagc agcgtgcaaa ccagcaacgg cgcgcggctg tagccggcgt
     5461 ccggcgggga gcttactcgc ggtatttgcc cgttgcccga agccattgct cgatcagcgg
     5521 cagcatcgct cgcaaggcct ggccgcgatg gctgatggca tgcttctgtt gcggcgtcaa
     5581 ttcggcggcg gtcaggccca attccgacac aacgaaaacg ggatcatagc cgaagccgcc
     5641 ggcgccgcgc ggcgcgtcgg cgatgcggcc ctccagcagg ccggtgctct cggccaagat
     5701 ttcgccaggc cgggcaagcg ccatgctgca gcggaagcgg gccgtacgtt gttggggcgg
     5761 cacgccggcc agggcggcca ggagttttct gatgttggcc gccgaatcct gctgcgggcc
     5821 ggcatagcgc gcggagtgca cgccgggcgc cccgcccagg tagtcgacct cgagccctga
     5881 atcgtcggcc agcgtcggca ggccggtggc cgcggagtag tgcagggctt tcaggcgcgc
     5941 gttgtcggcg aacgtggcgc cggtctcaag cgcgtcgggg acgtgcggga actcggccaa
     6001 gctgcgccac aacagcggca gcggcgcggc gatttcctgc aattcgcgca acttccccgg
     6061 gttctttgtc gcgagcagca gctcattcat atttggcacg ggggcgatct gttttggcgg
     6121 cgctcggcac ctggactacg aacgactcgt acgggacgcg cctcttctgc ccggtcgtgg
     6181 gatcaggaac gagctcgttg acgcgcctgc cgttctggta actgacggga aactgttcct
     6241 gcagtttgag caagcgctgg tcgacaatct tattgctcac cgtcagcacg ccggagagcg
     6301 gattggtctc tgacacagtc tgaatggctt ctttggggaa gagccccacg tagaccgagc
     6361 tacgcgtcgg tccgtgatag tagtaggcct ctacgccctg ctcgcgcagc tcgcggcagt
     6421 actcttcggc caagtacttg gggttggtta tgggcccttc gcagtagaag accgccacat
     6481 gcagcgtcca gtaccctttg gcgttggcca gatcccactc cggatgccgc gatcgcccgc
     6541 tgggcagttc ctcgagcgag gcgtagatga agggccagca gtcgctgccg ccgacgttca
     6601 tgcacaggct gcgtatgagg ttcaggtcgg ccatcgcatc ggggcggtaa gtcgcggtac
     6661 ttccgccgat ttcgacggtc cggcgatagc ggccgtaata aacggaggat tcgtgctcgt
     6721 tatgaatgac ctgcaccagt tggggcttaa ggccttgaac ctgcttgagg gcctcactct
     6781 cctgctgcgt gattctgaac cggtcggcgc cgtggcgtgt atcgcagcga atcgcccaga
     6841 acacctcgga ccccggcgtg gggcttaaac ccagccccgc cccgccggcc gtgcagccgg
     6901 tcagcgaaag cagaacgcac gcaagtggca gcaatgaacg ggggttacgt tgcatggcgt
     6961 aagtggtagc atgccgggaa cggcttggca accagcggcg agcgatctcc tggatcaagg
     7021 gaacattgga ggcctctttg gtggctgctt tgagtgaaag gcctgtgaac cgcaaggccg
     7081 aacgttgtcc gcgatgcaaa tacccgtacc acggtttgcc cgcggacagc gcctgtccgg
     7141 ggtgcggcat gcaggttcgc accggtgccc tggtcttcta tggaccgcgg cgcccgttcg
     7201 cggtgcttga cgtcctctat ctcgtccttg ccggctggtt tgccctagcc ctgccgggga
     7261 acatttcgcg gcaggacatg atcttgtttc tgtgcaacgc cgtggccgcg ttgctcgtgg
     7321 cgctgctgtg gtggagtagc cggcgtcgaa gatactgcta cgtggttgtg gacggagaca
     7381 gggtgcttta cgggcccgga agagacgcgc ctacggagct acgcttgcac ggggtatggc
     7441 gcgccgaata ctccgcccta gcaagagagg tgtgtctgcg cgatgcgtgc gggcgcacca
     7501 tcgcaacggt gccctgcccc tgggacagtg gagccaccgg gcaccagatg tgtgaggctc
     7561 tgaaccgcga gattgagcgg ggcacaagca ggccagacgg ctgatnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnggccg tcgtcgtcat cttcggactg ccgatcctcg
     7741 ccatcatcat tgcgggacga accagttcgc ggctgtacga cctcgagcag caactgaagc
     7801 aactgcggag cgacctgcgc tcctccgctg cgcgcagcga gcggtccgcc cccacgacta
     7861 tccgccctgc tgacgagcat gctcaaacga ttggcgtgca gccgctagcg ccggagcccg
     7921 cgctttccgt catggacgcg ccgtcgccct gcgaagcctc cttccccgag gaggaattca
     7981 ccgcgccgcc ttccacgccg gacacactgc acaccgacac aggccaacca atgccgactc
     8041 gcgcggccgg gcctgccgca agccgcagat tcgaggaaca ggtgggaaca cgcctgccgg
     8101 tctggatcgg ggtgaatcgg ctgccgaggc aagactcgag cgcagcctgt ccttcgcggg
     8161 cgtcctggcc gggctgttta tgctggcgct gctcgtcagt gccggcaact tctcaaatac
     8221 cgactggctg tttctgggac tgctcggcgc gggacttatg gtgctgggcc gccttgatgc
     8281 gcggtatgag ctactgccct ggcttgcggc cgccgccgga gtagcgctcg tgatcgcttg
     8341 gcgaatactg tccgacccgt ggaacacggc ccgttttgca ggcgtgacgc tcggcctcgg
     8401 cacgctctat gcgctgggcg cctacgcagc catgtgggca accaggcggc cgggcctgtg
     8461 ggcgggcctt tcgggcgccg cagcgatcgc gcatttcctg ctggcgtact gggcattgcc
     8521 ggagccgccg ttcgcctcgt cgtggggtgt gagctgcgta ttgctggccg caatctacgt
     8581 tgctcttagt gcgccagtca tcaggaggcg cgaaaccgct gctgcctatg atgcagccct
     8641 gggggcattg ctgacggctg ccaccgtttt tgtcagtctg gcgattccga tcgagcttga
     8701 acgcgcttgg atcgcggtgg cctgggccct ggagattccg gctctggcgc tcgtcgccgg
     8761 ccggctgcgc gtgccggttc tgcgctcgcg cgggcgttgc gtcgtgggcc gccgacgggt
     8821 tagtcccgta gggacggcct gatcgtagcc cagcgtgaaa cgctgggata ccgcccagca
     8881 cgggcccgta gtcctgtagg gacgattgag cgggcaccgc ggcagcggga ggttcctggc
     8941 ggcatttcgg ggcgatcgcg acttgatcgc cactacggcc cctgctcggc gccgaaacga
     9001 gccgccgggc cgaagcgctg ttcagcggct gcacacgcga gtataacctg acgcgggccg
     9061 catgcttgtg gctgggctcg cagagaggtt ggccgcggta ccagaatacc cgcgtgtggc
     9121 gcacaaggag ccggcgattt ccgacacgcc gctgctcgcg acgcgccagc gcattccggc
     9181 tcgggcgctg ctcgcatccg cgaagtgcgc gaccggtaca acagaccgga cgacacaacg
     9241 gaccggccgc gtcggcggcg tgctgcccgt gccggctagc cagccgggtt tgaggaaggg
     9301 acgtgcttct ggaggcagct ctcacggaga tacccgcatg aggtgggtca ggttcactct
     9361 cgcatccgca ctgctcgtga gtgtcgccgt cttcaccgcc gtcaagtttg gcagcctgaa
     9421 ggttcgccag ctccaaaatc aagtgaacca gcttcagcag gagcgacaga agctgatgga
     9481 gtacgccgag cgcctgagcg cgacgcggcg cgtggcgcag gtcgacgtgc tgcgacaata
     9541 ccacgatgcc gccggcaatc cggtaaccgc cctggtctgg caggagatcg ggccggacgg
     9601 cactctcggc cgccccgtcg ccgtggaggc gaccggcacg ctcgtctatt tcgaagctct
     9661 cgtgatcaag ttcgcccagc aatacgtcgg cgaaggcgac gcggagcgcg gcacgagcct
     9721 ggccatgttc cgccgcatct tcggcgaccg ccaggcgcct gagtccgccc ccgatctcga
     9781 ccgcgccgcg cgtcccccaa atacgcaatc gagtgcggcc gccgcgctgg aagacgaact
     9841 ctgggccaag ttctgggaat tagtggacga tccgcagctc gctgcgaaat acggcgttcg
     9901 cgtcgcacaa tgcgaggccc ccgccgtccc ggtacgcccc ggccaagtct gggaagtgtc
     9961 gctggacgcc gccggcggcc tgaatctcag gctgatcgga acccgcggcg agcccctaag
    10021 cggccgcggc caacaaactg cggcggccgg aatacagaac gcggcgtggc cgcggccggc
    10081 ggcgccgtaa gctattccca gttcacgccg aactgcaaga ccgccggaat gcgctcgggc
    10141 gtgtctacga aaaactcgac gaaggtggag ctttccggct cgcggtagaa gagcgtgagc
    10201 tggtattcga agccgctgtt gcactcgagc ggcgcgatgt cgaaatccac catcccgccc
    10261 agatggtgga acgtcagttc gctgggacac cgaattatga acacctccgc gtcgtcggtc
    10321 cggttcgttt tccacgactc gaagtgcccc agcaaataac cctcgcgcca agccagccgg
    10381 cgccgcgctg ccggatcaac cgtgatcgac ggagccgcct ggaccgtgaa gcgcaccacg
    10441 tccaccgcct cacgcccgtg agcatcgcgc accgtcagtt cgaattcgta cgtcgcatcc
    10501 ctttccggcc actcctgggc cagcccggcc gcaactgtat tcgccgcgcg cgggttatcg
    10561 atcagcaggt cgaccggtcc gctgagttgc cgccaagaat agctgagttc cgtgccgtgg
    10621 ctgcccgtcc cgtcgagtgt gatttcgtga tcccccatcc agaccgtgcg gtccggcccc
    10681 gcatcagcgt gcggcgtcgg gcttgcggcc ggcaaatcct gctcaggctg aagcgactca
    10741 ctcgggggct cggtgtccac cggcggaagg ctcgccgaat tatccggctg aggcgcgagt
    10801 gtgacctcgg gttgctctgc gggccgggtt ggcagcggcg gaggcggctc ctcgtcatag
    10861 gccgtgtatt caaagcggat ttcggtcccc gtctcgctgg agctggccgc gcccaccggg
    10921 cgaagcagat agcccttcac gaagatgatg ccggccagac acgccagccc gccaacgagg
    10981 aaaaacgagc gcatcgcctg cctcagccgg gccgcataaa agtacggccc cggtccttaa
    11041 ctagaatcgg atgcgctgca ttccagcaac agtcccgggc gtgcaaattc ttaacgcgcg
    11101 cgctcgaatg gccggatggg agccggcctc aggcgctgaa ccccctgctg agcgcagaat
    11161 cgccgctcgt attacccccg gggggcgccg cgggcagcgc aacgccgcga gcgctgccgc
    11221 gcggctggtt tcgagcgttt agtgcgtcgg attatggctc gtcgtcgtcc gcctgctcct
    11281 gctcgccctg cgcctcggag cccttgaacg ggttcttgcc gaacttggcg gtataattgc
    11341 cgcaactcgg gcatttcagg tagccgccct gcatatcgga cttcatggcg tccacttcgc
    11401 tggcgggcac tttctcggcc agagtcccgc agtttttgca ggtgacgtag tagtccttaa
    11461 agcggtggac gacctgtttg acctccgcgc tggtgctgcg gcacttccac gtccagaagc
    11521 cacccgaaac taccgcgagc aggaccaaca cgattatgag gttcttgttc atctcagccc
    11581 gacgtagcca tgcgttcatc ctaatatctc cacttcaact tttttcggtc ggcacgtgca
    11641 caatatagag cagtgcccgc gtttgtgcca cacgccgtgg cttttcaacg ccttgtttcg
    11701 ttcaggtttt ttaaccgttt actcccaaga gggccaccag cgagccaata tgcgctggtc
    11761 tcctattccg cttgcggctc gaactgcagc gaatcccgca agcg
//
LOCUS       DAOSYS010000099        11689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4674_length_11689_cov_5.058277, whole genome shotgun sequence.
ACCESSION   DAOSYS010000099 DAOSYS010000000
VERSION     DAOSYS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_11689_cov_5.058277"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..714
                     /locus_tag="PLU99_07790"
     CDS             247..714
                     /locus_tag="PLU99_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO16995.1"
                     /translation="MNTNTRNARVVICLILAMTAGARILLWLEPTPVISARATPLTAQ
                     AGVPIEEILIEYVQPGDGVVGPACLIMPDGNCSFEQGGSSVRLVVMGTEQGSLPEEQK
                     QSLLGILYSIACGRGSRTPAIHLDANSDARVSPGVPAQARELCDMLVAKRFLD"
     gene            785..1672
                     /locus_tag="PLU99_07795"
     CDS             785..1672
                     /locus_tag="PLU99_07795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO16996.1"
                     /translation="MASMTTMSEIPQIRKYELGSRIGRGAGATISLAYERTTRQQVAI
                     KHVVCRNPDDARFIVQAENEFHVAQRMDHPYLRKCYELIRSRRWLKTRELFLVMEYVD
                     GERLEDRCPERLGEMVSIFTQVATGLHAMHMAGFAHADIKPTNILLEKNGGLKIIDFG
                     QSCPLGHRKTRLQGTPDFMAPEQVARQRIDHRTDVFSLGATMYWAVTGKWFKTLMPAG
                     PAATKKIALEQYRGSLPPRELNPLVPLSLSRLIMECCESNPELRPPDMRKVASRLEVV
                     QHLLDQKSASAVIRPAGKV"
     gene            1674..2558
                     /locus_tag="PLU99_07800"
     CDS             1674..2558
                     /locus_tag="PLU99_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO16997.1"
                     /translation="MGAKLPGQTANDLDNARVAADRLQQAAALIREDPERQGNLLRFG
                     SAGQLVMTGDLHGNLRNFDKLQRFCRLERSPGRRVVLQELIHDDPLSAPVDLSIRLLV
                     RAAAWKCDFPDNVYFLQSNHELSQLRRHEISKGGRSVIHDFERGVQQLYGPEADLVLA
                     AADDYLRSLPLAARLANRVFLSHSLPDLLSVGLFDPSIFERELTERDMEPGGSTYALV
                     WGRYHSPQLLELLAQRLDADIFITGHMPQEMGYAVVGRLIILASEHAHGTFLPIDLSR
                     RYTVDELAGAIRKFVEIE"
     gene            2790..4334
                     /locus_tag="PLU99_07805"
     CDS             2790..4334
                     /locus_tag="PLU99_07805"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO16998.1"
                     /translation="MTQATVLESKARETLHQQLQARIDSLSYLPTAAAVAMKFVELGQ
                     NPDAEPGDYARVIGADSSLSTKLLALANSSWAGVRNRVTTVKMAVNLLGLGTVRTLAI
                     SYCLSGLHNELHLSQAESQIFWQISLWKAVAARICASLTDPKRADEAYVAGLFQDFAL
                     SVMYSVARERYLCLLQDRGLSAAALLAAERECFGVDHADAGRTIALKLELPELFVDAI
                     AFHHDPKRLAELVANPILREGASAAASLPHACNAWNREDSDALAALLARCTPPVELAD
                     FVSKVQTEFTHLFSFFNEGKVPEGKLPDLLAATAREAADNTTRLVGTVTDMVREAAEA
                     GAQLHRRMTSLESEATSDQLTGVLNRNGFTTRSKELLAKAARYGTSIAVCYLDLDHFK
                     QLNDTYGHALGDAVLRSAVSVSRSLLPQQAIMGRLGGDEFVILLTGCSQESARDIADK
                     VVSTVSKQTVAHDGQQLHVAWSAGLLYIRPTNEPQDIDQLLKAADQLMYVAKRRSGNA
                     VEMAVR"
     gene            complement(4379..5677)
                     /locus_tag="PLU99_07810"
     CDS             complement(4379..5677)
                     /locus_tag="PLU99_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO16999.1"
                     /translation="MPADDDAQMIFAARQAMAAAARGLAAYHANACVKCGLCGQSCHV
                     FLAEPQPENLPAAKAGEVIRVYRRYHSLLGKLLPALVGARDLTPQTLDQLVEAVYGRC
                     TGCGRCGLHCSIGLDVASVIRAGRQVLAAVGKVPDGLQRTVNNQLETGNQMAIPRDEF
                     MSTAEWLSEDLQLETGRKEAVIPVDQPGRRVLYLVNPREVKFFPLSLSAAAGVFTAAG
                     ESWTLSSKIYDVTNYGFFSGDDAAAGELVRRVVEEAQALGVQEIILGECGHGFRAFRW
                     EGPNWTRQSYPLPVRSVLELLDEYLAAGRIRVDPAKNPARVTLHDPCNLVRWGGVSEP
                     QRRVLGRCCADFAEMHPNRRNNYCCGGGGGMLSMSEYGQRRVASGQLKAEQIWRTGAK
                     IVATPCHNCADQLLEINKHYKLGVEIKTVVELVYGALVWP"
     gene            complement(5681..6307)
                     /locus_tag="PLU99_07815"
     CDS             complement(5681..6307)
                     /locus_tag="PLU99_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17000.1"
                     /translation="MNALRIMIAGSALWAFMLLTFQVLTARGGGRPDFSRPAGRPAAG
                     AWYNFTTGMLPWNKESAWLHLAEFGLGIVFHAGVLLTLASVFIMAATPTFGARLLALL
                     WPLNALSLAAGIALLIRRVRSELLRAISVPDDYLAVSVTCGLLIVALAFSFFGQNPAP
                     PLAYTSLLLIYLPLGKLRHAVFFFVARTNYGCRLGHRGVYPPPRSATE"
     gene            complement(6350..6658)
                     /locus_tag="PLU99_07820"
     CDS             complement(6350..6658)
                     /locus_tag="PLU99_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOO17001.1"
                     /translation="MLVEGKEIEFDKEGFMLDPNLWDDKVATAIACEEGIEQMSDQHW
                     SIVNYIRNHWKEHDLAPSVRMICKECGVSVRDVYKLFTSGPARGACRIAGLPKPDGCV
                     "
     gene            complement(6733..8121)
                     /locus_tag="PLU99_07825"
     CDS             complement(6733..8121)
                     /locus_tag="PLU99_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17002.1"
                     /translation="MHRSIAVAEAETRAVAAARQAIPRTRPRTRWADAALLGLLAAAA
                     GAAAWYGSCGIDEPLARRLPGDVWFEADCRRVFDNLAYFRSVQLRSTTHPLFTLWIQP
                     PVALAQWLGLPPILAVRALRALTAAGCAAALYTLLRVIGCRRLDSVALCILTGVSSAA
                     VFWFVVPETYPPGLLSILVALNIAAFAAARRSLAWDLLAQLATFSFTITNWMAGLLVT
                     FARWPWRRALAITGLALCLLQILIVLQNQVYPSAALWLNPLPERIYVLHPAAGGPARV
                     LPSFVFHSMVMPRIAITDRAHWVGPMLTVQESWPGSAGPWSIAAVVAWTALLSLGLYG
                     LATLRGQRRLRTVLGLTLAGQLVLHLLYGLETFTYACHFGPLLVVVAALATLTRARRV
                     ALGLILTLIVCVGINNLAQFREARTWVEQRAAAAQRTDARSVRAKLTAADEARPSPDF
                     RSVSSPQPRLPD"
     gene            complement(8501..9508)
                     /locus_tag="PLU99_07830"
     CDS             complement(8501..9508)
                     /locus_tag="PLU99_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOO17003.1"
                     /translation="MRAYQFDAFGLENLKLVDVAEPVPGPGEVLLQVRAISLNYRDLM
                     VIRGQYNPHVRLPATPISDGAGVISAIGPGVTQVKVGQHVCSHFISGWLDGRFRAEYT
                     GTTLGTPGPGLAAEQVVLPTAAVLPIPEGYDFAEAATLPIAALTAWSALRTIGNVTAG
                     QTVLTLGTGGVSIFALQMAKLMRAAVIVTSSSDEKLERARRLHADYLINYKSRPDWEN
                     LVLDFTDGQGADLTVETGGAGTLSKSMRATRTEGIIALMGALTGLKSELDLRLVLSRR
                     LHIAGVFVDSRAAFEAMNRFIEDHDLKPVISQRFSFEQLPAALRTMEAGEHFGKIVVE
                     M"
     gene            complement(9643..11364)
                     /locus_tag="PLU99_07835"
     CDS             complement(9643..11364)
                     /locus_tag="PLU99_07835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17004.1"
                     /translation="MAPLARWGLPSRQADELLFGGAPPWSADRYHTAAALQVRQERSG
                     GADTDLDPLTQRDRILDLTATTAARAEILRRYRLFSRQPDEMITFMALQRMRPRQFDF
                     DPRLYQYGGAFIYLVGTAIGASATLGITHLSSDPALYLEQPEEFARFYLAARLVTLAF
                     GALLLVAVWKLADRLAGTLAAWVATLLLACTPVFISGVLEAKPHVPSACMILWAALAA
                     LDYYDRGRRRDAVRLGLFAGCGFGLVLTGIVAAAIWPALLIARRRALASEAGRSPAGS
                     LLRHLALGAGIALAVYVLTNPYIPYNYLFNRAALASNIENSTAMYAGQIRQAGAGAVR
                     VGELLLESAGPGLLLTGLAGLLWLARRQPRQLATTASSGAAMLLIAVLLGAHKPAEYA
                     RFLILPVSIFAIGAGVAIGALAGVVRAVRSADVARCAPWRRPLALVVTVVVVATMKTP
                     IYVRSFIDDAAGQDESRRRAAELLSELTAAGEPIAVIQEPAPYAVPPLDFAHRQILWL
                     PPVRPAEIDPQSLPPWLVFTTDDATDYQDAWWRQYYRPAAEIPSQPRGYSRITWANKP
                     VFILQKK"
     gene            complement(11463..>11689)
                     /locus_tag="PLU99_07840"
     CDS             complement(11463..>11689)
                     /locus_tag="PLU99_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOO17005.1"
                     /translation="FHAATIAALAGVGLTAGLGWLYLTGVALVALLLAVENSLVRPHD
                     LRYVNVAFFTINGIVGVVLGLLGVLDIILT"
BASE COUNT         2206 a         3684 c         3833 g         1966 t
ORIGIN      
        1 caggattttg aaaggggcaa caaaatccaa gagcgagcgg tgcggcggac ttggccggac
       61 gctcgcgaat ctgaatactt cctggtgaga ggtggcgctt agggcaggca ggaaaccgat
      121 aagctaaatg ccggtgaatt ctcatggatt agcgggactt ctgggcgtta tccgttgcat
      181 ttgcccgcgc cccgcggtat aagtggccct gcaaggacgc tctctggagt ggagcgagga
      241 aggctcgtga atacaaacac ccgcaacgcc cgcgtggtca tctgcctgat tctggcgatg
      301 acggccgggg cccgcattct gctctggctc gaacccacac cggtaatatc cgcccgggcc
      361 acaccgctca cggcgcaggc gggtgtaccc atcgaggaga tcctaatcga atatgtgcag
      421 ccgggggatg gagtggtcgg gccggcgtgc ctgataatgc cggacggtaa ctgcagcttc
      481 gagcagggtg ggtcgtcagt gcgcctggtg gtcatgggca cggagcaagg ttcgctgccg
      541 gaagagcaga agcagagcct gctcgggata ctgtacagca ttgcgtgtgg ccgtgggtcg
      601 cgtaccccgg ccatccacct cgatgcaaac tcggatgctc gcgtgagtcc gggcgttccg
      661 gcgcaagccc gtgaactctg cgacatgctg gtcgccaagc ggttcctcga ctaacctact
      721 tggaacgcgc gtagaatagg ggtgtgtggc gcgcggcgcg ggccgcggag tgttacgccc
      781 cgccatggcg tccatgacca cgatgagcga gattccccaa attcgcaagt acgagttggg
      841 tagccgcatc gggcgcggcg cgggcgcgac gatcagcctg gcctacgaac ggactactcg
      901 gcagcaggtc gccataaagc acgtcgtttg ccgcaaccct gatgatgcgc gcttcattgt
      961 acaggcggag aacgagttcc acgtggcgca gcggatggat cacccctacc tgcgcaagtg
     1021 ctatgaactg atccgcagcc ggcgctggct gaagacgcgc gagttgttcc tggtcatgga
     1081 gtacgtggac ggggagcggc tggaggaccg ttgcccggag cgtttgggtg agatggtcag
     1141 cattttcacg caggttgcga ccggactgca cgccatgcac atggcgggct ttgcgcacgc
     1201 cgacatcaag ccgaccaaca tcctgctcga gaagaacggc gggttgaaga tcatcgattt
     1261 cgggcagagc tgtccgctgg ggcaccgcaa aacgcgtctg cagggcacac ccgacttcat
     1321 ggccccggag caggtggcgc ggcagcggat cgaccatcgt accgacgtct tcagcctggg
     1381 ggccacgatg tactgggcgg tgaccggcaa gtggtttaag acgctgatgc cggccgggcc
     1441 tgcggcgacg aagaagattg ctttggagca gtatcgcggg agccttccac cgcgcgaatt
     1501 gaatccgctg gtgccgctgt cactttcgcg gttgataatg gaatgttgcg agtcgaaccc
     1561 ggagttgcgt ccgccggaca tgcgcaaggt ggcctcgcgc ctggaagtgg tgcagcacct
     1621 gctggaccag aagagcgcct ccgcggtaat ccgtccggca ggcaaggttt gagatgggcg
     1681 ccaagctccc cggccagacc gcgaacgacc tggacaacgc ccgcgtggca gccgacaggc
     1741 tgcagcaggc ggccgccctg atccgcgagg acccggagcg gcagggcaac ctgctgcgct
     1801 tcgggtcggc cggccagttg gtgatgacgg gggacctgca cggcaacctg cgcaacttcg
     1861 acaagctgca gcgtttctgt cgtctggaac gcagtcccgg gcggcgggtg gtgttgcaag
     1921 agctgattca tgacgacccg ctttcggccc cggtagatct gtcgatcagg ttgctggttc
     1981 gggcggcggc gtggaagtgc gacttcccgg ataacgtcta ctttctgcaa tccaaccacg
     2041 agttatcgca gcttcgacgg cacgaaatca gcaaaggcgg acggtcggta atacacgatt
     2101 tcgagcgcgg cgtgcagcag ttatacggcc cggaggcgga cctggtgttg gcggcggcgg
     2161 atgattatct gcggtcgctg ccgctggcgg cgcggctggc gaaccgcgtg tttctctcgc
     2221 atagcctgcc ggatttgctg tcggtcgggc tgttcgatcc atcgatattt gagcgcgagc
     2281 tgactgagcg tgatatggag cccggcggct caacgtacgc gctggtctgg gggcgttacc
     2341 attcgccgca actgctggag ctgctggcgc agcggctaga cgcggacatt ttcattaccg
     2401 ggcacatgcc gcaagaaatg ggctatgccg tggtcgggcg cttgattatt ctggccagcg
     2461 agcacgccca cggcacgttc ttgcccatag atctcagccg gcggtatacg gtcgacgagt
     2521 tggctggggc catccgcaag ttcgtcgaga tcgagtaact cacggctgcc ggtttgttct
     2581 cggccggcga aattcaagta cacagggccg aatgggggtc tgatcggcga atgaccggcc
     2641 ttgctttaga gaaaaaagaa gtgaatgcgc cgttgcgcat ggtcgatcac acacaggcgt
     2701 cgggtgtgcg gtaacaggcc ggcgcgcccg tcagccgcga ggccgttctg agacgcgtaa
     2761 gtcttacaac cgggcaacag gtataaaaga tgacccaggc taccgtcctt gaatcaaagg
     2821 caagggagac attgcatcag cagttgcagg cgcgcattga ctccctgtct tacctgccga
     2881 ccgccgccgc ggtggcaatg aaattcgtcg agcttggcca gaatcccgat gcggagccag
     2941 gcgactatgc ccgcgttatc ggggctgact cgtccctgag taccaagcta ttggcgctgg
     3001 ccaactcgtc ctgggcgggc gtgcggaacc gcgtcacgac cgtcaagatg gcggtaaacc
     3061 tgctcgggct gggcacggtg cgcacgctgg cgatcagtta ctgcctgagc ggcctgcata
     3121 acgagctgca cctctcgcag gcggaatcgc aaatcttttg gcaaatctca ctttggaaag
     3181 cagtcgcggc ccgcatttgt gcgagcctga ctgatcccaa gcgcgcggac gaggcctatg
     3241 tggccggtct gttccaggac ttcgcgctaa gcgtcatgta ctcggtcgcc cgcgaacgat
     3301 acctttgcct gctgcaggat cgcgggctca gcgccgccgc cctgctggcg gccgagcgcg
     3361 aatgtttcgg tgtggaccac gccgacgccg gccgcaccat tgccctgaaa ctcgaactgc
     3421 cggagttgtt cgtcgacgcg atcgcgtttc atcacgatcc caagcggctc gcggaactgg
     3481 tcgccaatcc gatcctgcgg gaaggcgcca gcgcggcggc ttcgttgccg catgcctgca
     3541 atgcgtggaa ccgcgaggac tcggacgccc tggccgcgct gttggctcgt tgcacgccgc
     3601 cggtggaatt ggcggacttc gtgtcgaagg tgcagacgga gttcacgcac ctcttctcgt
     3661 tcttcaacga gggcaaggtg cccgagggca agttgcccga tctgctggcc gcgacggcgc
     3721 gcgaggcggc tgacaacacg acgcgtctgg tgggcacggt cacggatatg gtccgcgagg
     3781 ccgctgaagc cggcgctcaa ctgcatcgtc gcatgaccag cttggaatcc gaagccacga
     3841 gcgaccagct caccggcgtt ctgaaccgca acggcttcac gacgcgctcc aaggagctgc
     3901 tcgccaaggc cgcccgttac ggaacctcca tcgcggtctg ttacctggac ctggatcatt
     3961 tcaagcagct caatgacacg tacgggcacg cattgggaga tgccgtcctg cgctcggcgg
     4021 taagcgtcag tcgttccttg ctgccgcaac aggccatcat gggccggctg ggcggcgatg
     4081 aattcgtcat tctgttgacc ggttgctcgc aggagagcgc ccgcgatatc gccgacaagg
     4141 tcgtgtccac ggtctccaag cagaccgtgg cgcacgacgg ccagcagttg cacgtcgcct
     4201 ggagcgcggg cttgctttac attcgcccga cgaacgagcc gcaagacatc gatcaacttc
     4261 taaaagcggc cgatcagctt atgtacgtcg ccaagcgccg ctcgggcaac gcggtggaaa
     4321 tggcagttcg gtaaacgccg cgcggccggt taggcgcccg cggtgcagcg ccgcgtgttt
     4381 acggccagac cagagcgccg tagaccaatt cgactacggt tttgatctcc acgcccagct
     4441 tgtagtgctt gttgatttcc agaagctgat cggcgcagtt gtggcacggc gtcgcgacga
     4501 tcttcgcgcc ggtgcgccaa atctgctcgg ccttgagctg tcctgaggcc acgcggcgct
     4561 ggccgtactc gctcatcgac agcatgcctc cgccgccgcc gcagcaatag ttattgcggc
     4621 ggttagggtg catttcggcg aaatcggcgc agcaacggcc caggacgcgt cgttgcggct
     4681 cagacacgcc cccccaacgc accaggttgc agggatcgtg cagcgtgacc cgcgcggggt
     4741 tcttggccgg atcgacgcgg atccgcccgg cggccaggta ttcatcgagc agttcgagca
     4801 cgctgcgcac cggcagtgga taggactgcc gcgtccagtt gggtccctcc cagcggaagg
     4861 cgcggaagcc gtggccgcac tcgccgagaa tgatctcctg tacgccgagc gcctgggcct
     4921 cctcgacaac gcgccgcacc agttcgcccg cggcggcatc gtcccccgaa aagaatccgt
     4981 agttcgtgac gtcgtagatt ttgctggaga gggtccagct ctcgcctgca gcggtgaaga
     5041 ccccggccgc ggccgagagc gacagcggaa agaacttaac ctcgcgcgga ttgaccaggt
     5101 acaggacgcg gcggccgggc tggtcgaccg gaatgacggc ttccttgcgg ccggtctcga
     5161 gttgcaggtc ttcggaaagc cattccgcgg tcgacatgaa ctcgtcgcgt ggaatggcca
     5221 tctgattacc ggtctcgagc tggttgttga ctgtgcgttg cagcccgtcg ggtaccttgc
     5281 cgacggccgc cagcacctgc cgcccggcgc gaatgactga ggcgacgtcc agcccgatcg
     5341 agcaatgcaa cccgcagcgg ccgcagccgg tgcaacggcc gtagacggcc tcgacaagct
     5401 gatcgagggt ctgcggggtc aggtcgcgcg ctccgaccaa tgcgggcagg agctttccca
     5461 gcagtgagtg atagcggcgg tagacgcgga tcacttcgcc cgccttggcc gcgggcagat
     5521 tttcgggttg gggctcagcg aggaagacgt ggcaggattg gccgcacagg ccgcatttca
     5581 cgcacgcgtt ggcgtgatag gccgccaggc cgcgcgctgc ggctgccatg gcctggcgcg
     5641 ccgcgaaaat catctgtgcg tcatcatcgg ctggcacggg ctactccgtg gcgctgcgcg
     5701 ggggtggata tacgccgcga tggccgagcc ggcagccgta atttgtccgc gcaacgaaga
     5761 agaacacggc gtgccgcagt tttcccaagg gcaagtagat caacagtagg ctggtgtacg
     5821 ccaagggcgg ggcagggttt tgcccaaaga acgagaaggc aagcgcgacg atcagcagcc
     5881 cacaggtaac agaaactgcg aggtaatcgt cgggcacgct gatggcgcgc agcagctccg
     5941 agcgaacgcg gcgtatcagc agcgcgattc cggccgcgag cgacagcgcg ttcagcggcc
     6001 agagcagcgc caacagccgg gcgccgaacg tgggggtcgc tgccatgatg aaaacgcttg
     6061 ccagggtgag cagcacgccg gcgtgaaaca cgattcccag gccaaattca gccagatgca
     6121 gccaggcgct ctccttgttc cagggcagca tgccggtggt gaagttatac caggcgcctg
     6181 cggcgggccg gccggccggg cggctgaagt cagggcgtcc gccgccgcgc gccgtcagta
     6241 cctggaaagt gagcagcatg aaagcccata gcgcgctgcc ggcgatcatg attcgtaggg
     6301 cgttcattcg gcgagaaccc ggcagctccc gatgggcagg taaaccctgc tagacgcacc
     6361 cgtccggctt cggcaggccg gcgatgcggc aggcgccgcg ggccgggccg gaggtgaaca
     6421 gcttgtacac gtcgcgaacg ctcacgccgc attctttgca gatcattcgc accgacggcg
     6481 cgaggtcgtg ttctttccag tgattccgga tgtaattgac gatgctccag tgctggtcgg
     6541 acatttgctc gatgccctct tcgcaggcga tggccgtcgc gaccttgtcg tcccacaggt
     6601 tcgggtcgag catgaacccc tccttgtcga actcgatctc tttgccttca accagcaccg
     6661 caagactcct atatcagacg aaacctgaag aaccggctag ctactatatc gccgagcgat
     6721 gatctcggcg agtcagtccg gcaggcgggg ctgcggcgag ctgacgctgc gaaaatccgg
     6781 gcttgggcgt gcttcgtccg ccgcggtgag ctttgcccgc accgagcggg catcagtgcg
     6841 ttgggccgcc gcggcccgtt gttcgaccca ggtccgcgcc tctctaaact gagcgaggtt
     6901 gtttatgcca acgcagacga tcagcgtcag aatcaggccc agcgcgacgc gccgcgcgcg
     6961 cgtcagggtg gccagcgccg ctaccaccac cagcagtggt ccgaagtgac acgcgtaggt
     7021 gaaggtttcc aacccgtaca gcaggtgcaa caccaattgg ccggccagcg ttaaacccaa
     7081 aacggtgcgt aaacgacgct ggccgcgcag cgtggccagc ccgtaaagcc ccaggctgag
     7141 caaggcggtc caggcaacta ccgcggcgat gctccacggt ccagccgatc ccggccatga
     7201 ttcttggacg gtcagcatcg gcccgaccca gtgagcgcga tcggtaatgg cgatccgcgg
     7261 cataaccatg gaatgaaaga cgaacgacgg cagcacccgc gcggggccgc cggcggccgg
     7321 atggagcacg taaatgcgct ccggcagcgg gttaagccaa agcgcagcgg acggatagac
     7381 ctggttctgc aggacgatca gaatttgcag caggcataac gccaagcccg tgatcgcgag
     7441 ggctcgccgc catggccagc gcgcgaaggt gaccaacaag ccggccatcc aattggtaat
     7501 ggtaaagctg aaggtcgcta gctgggccag caaatcccac gcgagcgaac ggcgtgccgc
     7561 ggcgaaggca gcgatgttta gcgccaccaa gatgctcagc aggcccgggg gataggtctc
     7621 cggcactacg aaccagaaaa ccgcagccga gcttaccccc gtcagaatgc agagcgcgac
     7681 gctgtccagg cgacggcagc cgataacccg caagagggtg tacagcgccg ccgcacatcc
     7741 ggccgcggtc agggcccgca gggcacgcac cgccaggatc ggcggcaatc ccagccattg
     7801 cgcgagggcc acgggcggct gaatccagag cgtgaacagg gggtgggtcg tcgagcgtag
     7861 ttgaacggag cggaagtagg ccaggttgtc gaagacgcgc cggcagtcgg cctcgaacca
     7921 gacgtcgccc ggcaggcgcc tggcgagcgg ttcgtcgatt ccgcacgaac cgtaccaggc
     7981 cgcagcgccc gccgcagccg ccagcaggcc cagcagcgcc gcgtcggccc accgcgtgcg
     8041 tggcctggtg cgcgggatag cttggcgcgc tgccgcgacc gctcgcgtct ccgcctccgc
     8101 cactgcaata gatcgatgca tcgccgatcc ttcgggtggc atcttacgaa catgcgctgc
     8161 tggatcgcaa acgggcggag gttaattcgt ccgcatgggt caagcttcgg tgccatgccc
     8221 aagcccgcgt tgcgggcgcg ggcatgccgt tgccaacgcc ccaaacctag ggccatcgcc
     8281 ccgcacctag cgcctttaac gagggcatgc ccgcgcttag agcttgggca tggcacccaa
     8341 cggcggagct ggtacccaac ggagggggcg gcactgcttg aagctgtgct ttcaagcagt
     8401 ggcacacgcg acatcttggt gacttgccta cgtccccgcg acttacctac ttccccacga
     8461 cttggctaca tctctacgac ttggctatat ctccacttgc ctacatctct acgacaatct
     8521 tcccaaaatg ctcgcctgct tccattgtgc gcagcgctgc cggcagttgt tcaaatgaga
     8581 aacgctgcga aatcaccggt ttcaggtcgt ggtcttcgat aaagcggttc atcgcctcga
     8641 atgctgcccg cgagtcgacg aatacgccgg caatgtgcag ccgccggctg agtactaacc
     8701 gcaagtccag ttccgacttc aggccggtca gcgcccccat cagtgcgata atcccctcgg
     8761 tgcgcgtggc ccgcatcgac ttactcagcg tgcccgctcc acccgtttcc accgtcaggt
     8821 ccgccccctg cccgtcggtg aagtcgagca cgaggttctc ccaatccggc cgcgacttgt
     8881 agttgatgag gtaatccgca tgcaaccggc gggctcgctc cagcttctcg tcgctgctgc
     8941 tggtgacgat gacggcggcc cgcatcagct tggccatctg cagggcaaag atcgatacac
     9001 cgcccgtgcc gagcgtcagg accgtctgac cggccgtaac gttgccgatg gtccgcaggg
     9061 cgctccaagc cgtcagggcc gctatgggca gcgtggccgc ttccgcgaag tcatagccct
     9121 ccggaatggg gagtaccgct gcggtcggca ggaccacctg ctcagctgcc aggcccggac
     9181 ccggcgtacc cagcgtcgtg ccggtatatt ccgcccgaaa gcggccgtcg agccagccgg
     9241 aaataaagtg cgagcacacg tgctggccga ctttcacctg agtcactccc ggtccgatgg
     9301 cggagatgac gccggccccg tcgctgattg gtgtggccgg gaggcgcacg tgcgggttgt
     9361 actggcccct gatcaccatg agatcgcggt aattcaggct tatcgcccgc acctgcagca
     9421 gcacctcgcc cggcccggga accggctcag cgacgtccac caacttcaga ttctcaaggc
     9481 cgaaagcatc gaactgatag gcccgcatgg tcggctccta gactaaagac cgcaccggga
     9541 tgataggcca agttgggttc aaatgcagcc ctgcgcgccg gaggtgcgtc ggacggcggt
     9601 aaaagagcgc gtggcaagct gcggctcgcg gtcttaacgg tcttatttct tctgaagaat
     9661 gaacaccggc ttgttcgccc acgtgatgcg gctgtatcca cggggctggc tcggaatttc
     9721 ggcggctggg cggtaatact gacgccacca tgcgtcttgg tagtcggtcg catcatcagt
     9781 cgtgaaaacg agccaaggcg gcagcgattg tggatcgatt tcggctgggc gtaccggcgg
     9841 caaccacaga atttgccggt gtgcaaaatc cagcggcgga acagcgtaag gggcgggttc
     9901 ctggattacg gctatgggct cgcccgcagc ggtaagctcg ctgagtagct cggccgcgcg
     9961 gcggcgcgat tcgtcctgcc cggcggcgtc atcgatgaac gagcgaacgt agataggcgt
    10021 cttcatggtt gccacgacta cgacggtgac aacaagtgcc agcgggcggc gccacggcgc
    10081 gcagcgtgca acatcagcgc tgcgcaccgc tcggaccacg cccgcaagag ccccgatagc
    10141 cacgccggcg ccgatcgcga atatgctgac cggcagaata agaaaacggg cgtattcggc
    10201 tggtttatgc gcgcccagga gcacagcgat gagtaacatc gctgcgccgc tgctggcggt
    10261 cgtcgcgagt tgccgcggct gccggcgcgc cagccatagc aagccagcca gcccggtcaa
    10321 cagcagcccg ggccccgcgc tttcgagcag cagctcaccc acgcgaaccg caccggcgcc
    10381 agcctggcgg atttgaccag cgtacatcgc cgtggagttt tcgatgttgc tcgcgagcgc
    10441 ggcgcggttg aacaggtagt tgtagggtat gtacggattc gtcaggacgt agacggcgag
    10501 cgcgatgccg gcgcccaagg cgaggtggcg gagaagactg ccagccgggg atcgcccggc
    10561 ctcgctcgcg agcgcccgcc tgcgagctat gagcaaggcc ggccagattg ccgctgcgac
    10621 gataccggtc agcaccaggc caaagccaca gccggcgaac aggcccagcc gaaccgcgtc
    10681 acggcgtctg ccgcggtcgt agtaatcgag cgccgcgagc gctgcccaca aaatcatgca
    10741 cgcggacggc acgtgcggct tggcttccag aacgccgctg ataaagacgg gcgtacacgc
    10801 cagcagcagc gtcgccaccc atgcggcgag cgtcccggcc aagcggtcgg ccaatttcca
    10861 gacagccacc aacagcagcg cgccgaaggc caacgtgacc aacctggccg ccaaatagaa
    10921 ccgggcgaat tcctcgggct gctccaggta caacgccgga tcactgctga ggtgcgttat
    10981 tcccagcgtc gccgaagcgc cgatcgcggt cccgaccagg tatatgaacg cgccgccgta
    11041 ctggtacaag cgcggatcaa agtcgaactg ccgcggacgc atgcgctgca aggccatgaa
    11101 cgtgatcatc tcgtccggct ggcgcgagaa gaggcgataa cgccgcagaa tctcggcccg
    11161 cgcggcggtg gtcgcggtga gatcgaggat ccggtcgcgc tgcgtcaacg ggtccaggtc
    11221 ggtatccgca ccgccgctgc gctcctggcg cacctgcagt gcggcggcgg tgtggtaccg
    11281 gtccgcgctc cagggcggtg cgccgccaaa gagcagctca tccgcctggc ggctcggcag
    11341 cccccagcgc gccagcggcg ccatcaggcc gaaccacgcc agggcggcca accaaacggc
    11401 gtaccggccc catcggggtc gcgcggacac cggcgacgag accgaattgg ccaccgcttc
    11461 actcacgtca aaatgatgtc gagtacgccg agcaggccaa gaactactcc cacgattccg
    11521 ttgatcgtga aaaaggcgac gttcacgtac cgcagatcat gcgggcggac gagcgagttt
    11581 tccaccgcca gcagcagtgc caccagcgcc acgccggtca ggtacagcca accgaggccg
    11641 gccgtcagcc cgacacccgc caaggccgcg attgttgccg catgaaagg
//
LOCUS       DAOSYS010000100        11562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4750_length_11562_cov_4.989572, whole genome shotgun sequence.
ACCESSION   DAOSYS010000100 DAOSYS010000000
VERSION     DAOSYS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11562
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4750_length_11562_cov_4.989572"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLU99_07845"
     CDS             <1..1072
                     /locus_tag="PLU99_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO17006.1"
                     /translation="QGLARFEDSRTLRVEGGDVAHVRFKHAIIATGSRAKRLPESIMP
                     ADCFIDSSQALDLPSIPKNLLVVGAGYIGLEIGQVYAALGSRVTVIEALPRIMPGADD
                     DLVRPLAARLKKQFEAIHTGAKLVSARRTGETVEVSFATSKGETKKSNFDAVLVAVGR
                     EPLSRDLGLENTKVTVDARGFIQVNAARQTTDKRIYAIGDVAGEPMLAHKASHEGIVA
                     AEAIAGRPSIFDARAIPAVAYTSPEVASCGLTEAQAAEQGRKIIVGKFPWGASGRAIA
                     MGRPEGLTKIIAEQDSRRVLGVGIVGEHAGDLIAEAVLAIEMGATAEDLAMTIHPHPT
                     TSETVMEAAESVLGTAIHSLRS"
     gene            1284..2894
                     /locus_tag="PLU99_07850"
     CDS             1284..2894
                     /locus_tag="PLU99_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17007.1"
                     /translation="MSDASLDPSLSSSLDPSTPVQPTIATPPGSPEARSLAFDLNSAA
                     ERLVCDLQRAASDGAAQDWSEFGALLLRLWGPPDGADALLLLRSGGPAGFGETVLPLR
                     LSAGWNLLTVDLDSVSRGHERRDMRQLEIIPVIGSKPATFHLDDLVLADRTQWYTGQD
                     AGPGELYAYSRGPRVFVGVHERFELVFAEGAISAWYATGPGSLAGLGGLGPWPVFVAN
                     DWSGSLEQGARGLTLAGPLDPGMRPAVTQRVLEASATRVVLEGSRELLLAADAADVNS
                     SAELRQCRSRYVVYRDGRVFVQISLEAGSTPWPSDRLGWAVLLDSGTDFAVAESPPAT
                     PEDVPANFALVTSNPPGADLLWAPHAAGDMRWRRFLPRTGSEPSLLLAGETQSQPVML
                     STHLLWFCPAGFNAEKAEEIASNYQEPLALQPQAGHLVTDVPADLNHDGYNESEGCYE
                     LALQRGRLRFDVLPGSTPRHEPLFRINGTDKTRCWVYADGQALPVETRDAAGQIMFTL
                     PGIIRSPRTIEVYALPEQPELQRPLEPQ"
     assembly_gap    3399..3498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3698..4867
                     /locus_tag="PLU99_07855"
     CDS             3698..4867
                     /locus_tag="PLU99_07855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOO17008.1"
                     /translation="MPKTKSKVTAKAGQRDRAAAGRRIVGLAKEVAGRIGQHEDPYVD
                     IPSRTLSNVRFNETKRIIELLDGKQRRFFFSLLGRRKGEGSQAKKFMQTIRVAEVCKR
                     LIDSNDSTSIRDLFYNLKVPIKTLDGKPARELIFADQSESDPIIEDIEVAVSALREEL
                     GVHAETKGAMVGPMTIVDNGDVINLANMGSGGWAVPSIVEPDVIEFRKADAKYVLLIE
                     KGAVWQRFNKDRFWESQKCMIIHGGGQPPRGVRRLIYRMHHELSLPVYVLVDNDPWGY
                     YIYSVLKQGSINLAFESQRMAIPAARFIGMSSFDADEFDLPSHVTMPLTEEDRRRARE
                     IREYPWFASKPWQREIKHMETLGVKLELEALSNKHFSFITEDYLPRKLKEKRWLD"
     gene            complement(4913..8506)
                     /locus_tag="PLU99_07860"
     CDS             complement(4913..8506)
                     /locus_tag="PLU99_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOO17009.1"
                     /translation="MFEPLPAQVNFSQAELQVLEFWRKHDIFGKSLELRRGAPRFVFY
                     EGPPTANGLPHPGHCLTRAIKDLFPRYQTMCGKLVERKAGWDTHGLPVEVEVCKQLGI
                     HTKEEIEAYGVARFVQKCIESVFRYVREWEEMTRRLGFWVNLEEAYVTYHQSYVESVW
                     WSLKTLFDRGLLYQGHKVVWWWAQGGTALSAGEVGEGYRTVDDPSVYVRFPLIAEGRR
                     DQGIEGSRDREGSPEGETRTELPHRRLPADAKVSLLVWTTTPWTLISNHFAAVGPDID
                     YLLVRDKTTGEYLYIASALVAATAEKVKHELEVVGVCRGADLVGLRYEPPFDSYSKTL
                     RDKTARLKEGGEAHIAWRVVPADFVALDTGTGLVHQAPAYGEVDFNLLQEERRRFVDF
                     DAVPLLNAVAPDGTFTSDAPDAYRGRWVKDCDKGIIQELRERGILYHQETIRHEYPFC
                     PRAEDDPLIQYARKSWFVRTSEFRDEFLKNNEKINWLPEHIREGRFGDFLRNNVDWAL
                     SRERYWGTPLPIWVCEKTGKMEAIGSLAELKAKPGATDDGYWEARAAEAKGRGETLLD
                     HLRVHKPYIDAWTYDSPFAAGARMRRVPEVIDCWWDAGSMPFAQWGFPHAPGSIQTFV
                     ERFPADFISEAIDQTRGWFYGLLAISTLIFGQGEGSRGLERHTNGQPEQLPVATAEYP
                     HPYRTCIVLGHVMGEDGLKMSKRTKNYKEPSYIFDHYGADAMRWYFYSGQAPWTSIRF
                     QEANIRDAQREFLLRLYNVFSFFNIYANIDGFTVNRDQGIKGSSQGQTATGQRPAAQR
                     SELDRWIISELHRTIKFVREKLDAFENYPAAGRLKDFVDALSNWYVRRSRPRFWKGEK
                     DQDKWDAYNTLYEVLVALAKLLAPFTPFFAEMMYQNLVGSAAGTNRDQGIEGSRDQEA
                     CAAKSSSDSSRLGVPNPLSVHLCDYPTADPALIDEKLATEMDLVRDVVSLGRAARAEA
                     KLKVRQPLESVEIVLARPEHTEWLAAHNDLISDELNIKRVGFAAEADHYVSYQLKADF
                     KALGPKFGKLAPRIAAALGTVDPLEARRSLSVTGTLVIDVDGQPVSLTSQEVQVRLQA
                     KPGWSAAQGRAGVVVVKTDLTPELKQEGLMRELIHAVQALRKDKDLPYEARITLYVEG
                     PAELREIVQRFESTISHECLTTAIVFGPPAEGQMQALKLDGLETRLTIATA"
     gene            8640..10277
                     /locus_tag="PLU99_07865"
     CDS             8640..10277
                     /locus_tag="PLU99_07865"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOO17010.1"
                     /translation="MAALVIYGADGRQGVWQLSQKPLTLGRAENCDIVLRFDGEVSRE
                     HARVWLDETGTVLVADLGSKNGTSVDAGETFRNETRPAYRSIRIGGQEVTIRGALRPP
                     ASAETRVAFVADAPERLTQSHFFPSTKRLDLSEQRLSLLMGLGQSLSGAFERKQLLEQ
                     ALDACCQALQFERCLIVLKTPRGEPESPVVRNVERDETGAFKVSRTLINRALLHGERA
                     VVNNPAVDLVGNITDSLVRFPICSALSVPIMQRDEILGAIYGDRVTRAATYSTQDVDF
                     LAAIAQQVGVGLANLRLLQDYVRLQKVDAELSAARKIQRDLLPAAPLAAGCVLIDGYN
                     EPSSEVGGDYFDYFPLDDTHVGFVIADVTGHGLPAALLMANFQAAVHVALTPDTPLPE
                     IAARINRLVCQNTAGSSFITAVLGKVDCRSGAMDYVNAGHPAPLLPGCANAGPDDRHN
                     SLPLGIDPGEDFRVEKLNPAEHAGVVLFYTDGLTELNNPAGDMLGIEAVRDALQKLPA
                     ASTELALRAALALARHHRGHAKGSDDLTLLAIQVTRP"
     gene            10526..>11562
                     /locus_tag="PLU99_07870"
     CDS             10526..>11562
                     /locus_tag="PLU99_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOO17011.1"
                     /translation="MPASTPTPVLLSLLQQAAARQASDIHLVSGYPATFRVNGRLERA
                     APKPLTSDDIRCLAESILPAAVFQTFEQTKNADCSLSVEKGGAPPLRFRANVYLARDG
                     WCICLRHIPNEIPTLDWLGFPKELAERLVAYQDGLVLVTGETGSGKSSTLAALIDLAR
                     RTRDAHIITIEEPIEYIHEVRGLSIVTQREVGRDVSSFADGLKYGLRQDPDIILVGEI
                     RDRDTAQMAISAAETGHLVFATMHTRDAKGALTRLVDLFPQDSQDDIRTQLALSLRSV
                     VSQKLLPPAEEGQRRVLALEVLHASQPVQVAIRSGRIETLDSALQTGARSGMIMLDDD
                     LRRLVREGKITT"
BASE COUNT         2105 a         3907 c         3400 g         2050 t
ORIGIN      
        1 tcagggcctg gcacgcttcg aagactcgcg cacgctgcgc gtcgaaggcg gcgatgtcgc
       61 ccacgtgcgc ttcaaacacg ccatcatcgc taccggctcg cgcgccaaac gcctgcccga
      121 gtcgatcatg ccggccgatt gcttcatcga ctcgtcgcaa gcgctcgacc tgccctcgat
      181 ccccaaaaac ctgctcgtgg tgggcgccgg ctacatcggg ttggaaatcg gccaggtcta
      241 cgccgctttg ggcagccgcg tcaccgttat cgaagccctg ccgcgaatca tgcccggcgc
      301 ggacgacgac ctcgtccgcc cgctggccgc gcggctcaag aaacaattcg aggccatcca
      361 taccggcgcc aaactggttt cggcacgccg taccggcgag acggtcgaag tgagctttgc
      421 tacgagcaag ggcgagacta agaagtcgaa ctttgatgcc gtcctggtcg ccgtcgggcg
      481 cgagccgctt tcgcgcgacc tcggcctcga gaatacgaaa gtgaccgtcg acgcccgcgg
      541 ctttatccag gtcaatgccg cgcgccagac caccgacaag cgtatctacg ccatcggcga
      601 cgtcgccggc gaacccatgc tcgcgcacaa ggccagccat gagggcatcg tcgcggccga
      661 agccatcgcc ggccggccga gcatcttcga cgcccgcgcg attcccgcag tggcctacac
      721 ctcgcccgag gtcgccagtt gcgggctaac cgaggctcaa gccgccgagc agggccgcaa
      781 gatcatcgtc ggcaagtttc cctggggtgc ttccggccgg gccatcgcca tgggccgccc
      841 ggaagggctg acgaagatca tcgccgaaca ggactcgcgg cgcgtgctcg gcgttggtat
      901 cgtcggagaa catgctggcg acctgatcgc cgaggcggtg ctggcgatcg aaatgggcgc
      961 cacggccgaa gacttggcca tgacgatcca tccgcatccg acaacctcgg agaccgtcat
     1021 ggaggcggcg gagagtgtgc ttggtaccgc tatccactcg ctgcgtagtt aaaccactgt
     1081 gcgcgaatct gcggatcgcg gcacaactgg ccgcgtgcgc ggcactgttg cttacaggcg
     1141 gctgcgaact cgccagcccg cgcctgcctg gtccgccgct cgacccagcc tcggtcgccg
     1201 cccactatcc cagcgttgaa ttctccggcg cgcaaacgct tgcagatttt cagaacccgg
     1261 ctcaagccca ctggttcagt atcgtgagcg acgcttctct cgatccctcg ctctccagct
     1321 ctcttgatcc ctccacccca gttcagccca ctatcgccac cccgcccggc agtcccgaag
     1381 cccgcagcct cgcgttcgac ctgaactccg ccgctgaacg cctggtgtgc gatctgcagc
     1441 gcgccgcctc cgacggcgcc gcgcaagact ggagcgaatt cggggcccta ttgctgcgac
     1501 tctggggtcc gccggatggc gccgatgcac tccttctgct tcgcagcggc ggaccggctg
     1561 ggttcggcga aacagttcta ccccttcgac tgtccgctgg ttggaacctg ctgaccgtcg
     1621 atctggactc cgtcagccgc ggccacgaac gccgtgatat gcggcaactg gagatcattc
     1681 ctgtcatcgg aagcaaaccc gccaccttcc acctggatga cctggtgctg gccgaccgca
     1741 cgcagtggta caccgggcag gatgccggcc ctggcgaact gtatgcgtac tcccgtggcc
     1801 cgcgggtttt tgtcggcgtt cacgagcgct tcgaattagt gtttgccgaa ggcgcgatca
     1861 gcgcctggta cgcgaccggg ccgggaagtc tcgccggact gggcggcctc gggccctggc
     1921 cggttttcgt ggccaatgat tggtcgggaa gcctcgagca gggcgcgcgc ggtctgactc
     1981 tcgccggccc gcttgatccc ggcatgcgcc cggcagtgac tcagagagtc ctcgaagctt
     2041 ccgctacccg cgtggttctg gaaggctcgc gcgaattgct tctggctgcc gatgccgccg
     2101 atgtcaacag ctcggccgaa ctgcgccaat gcaggtcgcg ctatgtcgta taccgcgacg
     2161 gtcgcgtatt tgtgcagatc agccttgagg cgggctcaac gccgtggcca tccgaccgtc
     2221 ttggctgggc cgtcctgttg gacagcggga ctgacttcgc tgttgcggag tccccgcccg
     2281 ccactcccga agacgtcccg gccaatttcg ctctggtgac gagcaaccct cccggcgctg
     2341 atctgctttg ggcgccgcac gcggcgggcg acatgcgctg gcgccgcttc ctgcccagga
     2401 cgggttcaga accgtcgctg ctgctggcag gcgaaacgca gagccagcca gtcatgttga
     2461 gcacgcacct gttgtggttt tgccctgccg ggttcaacgc cgaaaaggcc gaggagatcg
     2521 ccagcaacta ccaggagccg ctcgccttgc agccccaggc cggccacctg gtgaccgacg
     2581 ttcccgcaga cttgaaccat gatggctata acgagtcgga gggctgctat gagctggcgc
     2641 tccagcgggg gcgccttcgg ttcgatgtcc tgccaggctc gacgccgcgc cacgaaccgc
     2701 tctttcggat caatggcacc gacaagacgc gttgctgggt ctacgccgac ggccaggcgc
     2761 tgccggtcga aacccgcgac gcggccggcc aaatcatgtt caccctgccc ggcatcattc
     2821 gctcaccgcg cacgatcgaa gtctacgccc tgcccgagca gccagaatta cagcgcccgt
     2881 tagaaccgca atgacacgtg agccgtgtgc gttgaatgaa cgacgtgtgc gttgaacgac
     2941 gtctgcgttg aatgagccgc agtgagtagc ggagccggct cgtgtcgagc cggaatttga
     3001 ccagagtgcc gccaatcgcc caggccgcag gtccaccacg gatcactgcc ctcgctggcg
     3061 cttcgggctc ggatagtcgt tcgcaattaa gtcagcagaa cagaactgcg cggctggagg
     3121 tccgcagtgc ctcgggggtg ggagctccgg caaaagctgg tgcagtgacg tcgggtcttg
     3181 aaggcggcgt gatgaactgg gttgtgaaaa cgcgcgcaaa aaattgggcg gacgtcggtg
     3241 ggcgccgggg ggaaacgccg cagccgagca tgccgcccgt ggctcttaac ccgattgact
     3301 cgggttcttg tccttccaat taagtaatat acctcaaaaa tcaggcaaat caatcccatc
     3361 gtcagaaatt tgaaaatttt tctcgctcgc cgcaatgcnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnca caatgcgtcc aaaatcggcc ccgaatcatt aaaatggccc
     3541 gaaccattaa atcgcccccg gagcaggcga atggagggca gacgagcgaa gtcccttgcc
     3601 cccagcgtcg atcctcggca tcatcctgca acgatagcat ctgataatca gccggctgcc
     3661 cttttttcgg cccggcctgc gtgaaacgga gttcgcattg ccaaaaacca agtccaaagt
     3721 caccgccaaa gccggtcagc gcgaccgcgc cgccgccggc cgtcgcatcg tcggcctggc
     3781 caaagaagtg gccgggcgca tcggtcagca tgaggacccg tacgtcgaca tcccgtcccg
     3841 cacgctgagc aacgtgcgat tcaacgaaac caagcgcatt atcgagctgc tcgacggcaa
     3901 gcagcggcgt ttcttcttca gcctgctcgg ccggcggaag ggcgagggca gccaggccaa
     3961 aaaattcatg cagaccatcc gggtcgccga ggtctgcaaa cggctgatcg acagcaacga
     4021 ctcaaccagc atccgtgatt tgttttacaa cctgaaagtc cccattaaga cgctcgacgg
     4081 caaacccgcg cgcgagctga tcttcgccga ccaatccgaa agcgatccga tcatcgagga
     4141 cattgaggtc gcggtatccg ccttgcgcga agagctcggc gtccacgcgg aaaccaaagg
     4201 cgccatggtc ggccccatga ccatcgtcga caacggcgac gtcatcaacc tggccaacat
     4261 gggctccggc ggctgggccg tccccagcat cgtggagccc gacgtgatcg agttccgcaa
     4321 ggccgacgcc aagtacgtcc tgctgatcga aaaaggcgcc gtctggcagc gcttcaataa
     4381 agaccgcttc tgggaatcgc agaagtgcat gatcattcac ggcggaggcc agccgccgcg
     4441 cggcgtgcgg cggctgattt accgcatgca ccacgagctg agtctgccgg tttatgtcct
     4501 ggtcgataac gatccgtggg gctactacat ctatagcgtg ctcaagcagg gctcgatcaa
     4561 cctggcgttc gagagccagc gcatggccat cccggcggcg cgtttcatcg gcatgagcag
     4621 cttcgacgcc gacgaattcg atctgccgtc gcacgtgacc atgccgctga ccgaggaaga
     4681 ccgccgccgc gcccgcgaga tacgcgaata cccctggttc gccagcaaac cctggcaacg
     4741 cgagatcaag cacatggaga cgctcggcgt gaagctggag ctcgaagcgc tgtccaacaa
     4801 gcacttcagc ttcataaccg aggattacct gccgcgcaag ctgaaggaga agcgctggct
     4861 ggattgattc gagccgaccg ggcgaaggcg gcgcccgcga cgcacctgct gactacgccg
     4921 tggcgatggt tagccgcgtc tcgagcccgt ccagtttcaa cgcctgcatc tgcccttccg
     4981 ccggcgggcc gaatacgatc gccgtcgtca ggcattcgtg gctgattgtg ctttcgaagc
     5041 gctgcacgat ttcgcgcagc tccgccgggc cttccacgta cagcgtgatg cgcgcctcgt
     5101 acggcaggtc tttgtccttg cgcagggcct gcacggcgtg aatcagctcg cgcatcaggc
     5161 cttcctgctt cagctcgggc gtcaggtcgg tcttgaccac taccacgccg gcccgcccct
     5221 gggccgccga ccagcccggc ttggcttgca gccgcacctg gacttcctga ctggtcaaag
     5281 acaccggctg cccatctacg tcgatcacca gcgttccggt cacgctgagc gagcgccgcg
     5341 cctcgagcgg atcgaccgtc cccagcgccg cggcaattcg tggcgcgagc ttgccgaact
     5401 tcggccccag cgccttgaag tccgccttga gttgatagga aacatagtga tcggcctcag
     5461 cggcgaaccc tacccgcttg atgttgagct cgtcggaaat cagatcgttg tgcgccgcga
     5521 gccactccgt gtgctccggc cgcgccagga caatttcaac cgactccagc ggctggcgca
     5581 ccttgagctt cgcctcggcc cgcgccgcgc gccccagcga gacgacgtcg cgcaccagat
     5641 ccatctcggt cgcgagcttc tcgtcgatca aggccggatc ggcggtcggg tagtcgcaca
     5701 agtggacgga caacggattt ggaactccaa ggcgcgagga atccgaagaa gacttcgcag
     5761 cgcaagcttc ttgatcccta gatccctcga ttccttgatc cctgttagtc ccggctgcgg
     5821 acccaaccag gttctgatac atcatttccg cgaaaaacgg cgtaaacggc gccaacaact
     5881 tcgccagcgc taccaggacc tcgtacagcg tgttgtacgc gtcccacttg tcctggtcct
     5941 tctcgccttt ccagaagcgc gggcgcgagc ggcgcacgta ccagttcgac agggcatcca
     6001 cgaaatcttt cagccgcccg gccgccggat aattctcgaa ggcgtccagc ttctcgcgca
     6061 cgaacttaat cgtccggtgc agctcgctga taatccagcg atcgagttcg gaccgttgtg
     6121 cggcgggcct ttggccagtg gcagtctgcc cttggctcga tcccttaatc ccttgatccc
     6181 tattgacggt gaatccgtca atattggcat agatattgaa gaagctgaac acattgtaca
     6241 gccgcagcag aaattcccgc tgcgcatcgc ggatattggc ttcctggaag cgaatgctgg
     6301 tccacggcgc ctgcccactg tagaaatacc agcgcatggc gtctgcgccg tagtgatcga
     6361 agatgtacga aggctccttg tagttcttgg tgcgcttcga catcttcaac ccgtcctcgc
     6421 ccatcacatg gcccaggacg atgcacgtgc ggtaggggtg cgggtattcc gccgtagcaa
     6481 ccggcagttg ctcaggttga ccatttgtgt ggcgctcaag cccgcgcgac ccttctcctt
     6541 gaccgaaaat cagcgtgctg atggccagca gcccgtagaa ccagccgcgg gtctggtcga
     6601 tggcctcact gatgaagtcc gcgggaaaac gctctacgaa tgtttggata ctgcccggtg
     6661 catgcgggaa cccccactgg gcaaacggca tactgcccgc atcccaccag cagtcgatca
     6721 cttccggcac gcgccgcatc cgcgccccgg ccgcgaacgg gctgtcgtac gtccacgcgt
     6781 cgatgtaggg tttatgaacg cgcaagtggt ccagcaacgt ctcgccacgc ccctttgcct
     6841 cagccgcgcg agcttcccaa tatccgtcgt ccgtcgcgcc gggcttggcc ttcagctcgg
     6901 ccagcgagcc gatcgcctcc attttgccgg tcttctcgca cacccagatc ggcagcggcg
     6961 tcccccaata gcgttcgcgc gacaacgccc aatcgacgtt gttccgcaga aaatcgccga
     7021 accgcccttc cctgatgtgc tcgggcagcc agttgatctt ctcgttattc ttcaggaact
     7081 cgtcccggaa ctcgctggtg cggacgaacc agctcttccg cgcatactga atcagcgggt
     7141 cgtcttcggc ccgcgggcag aacgggtatt cgtgccggat cgtctcctgg tgataaagaa
     7201 tgccgcgctc gcgcagttcc tggataatgc ccttgtcgca atccttgacc caccgcccgc
     7261 gatacgcatc cggcgcgtcg ctcgtaaacg tgccgtccgg ggccactgcg ttcagcagcg
     7321 gcacggcgtc gaaatcgaca aaccgccgcc gctcttcttg cagcaggttg aagtcaacct
     7381 cgccgtacgc cggggcctgg tgcaccagcc ccgtacccgt gtccagcgcc acaaaatcgg
     7441 ccggcaccac gcgccacgca atgtgcgcct cgccgccctc tttcaggcgc gccgttttgt
     7501 ctcgcagcgt cttcgaatac gaatcaaacg gcggctcata gcgcaacccg accaggtccg
     7561 ctccccggca tacgccgacc acttccagct catgcttgac cttctcggcc gtcgcggcga
     7621 ccaacgcgct ggcgatgtac aggtactcgc cggtcgtctt gtcgcgcacc agcaggtagt
     7681 cgatatccgg cccgaccgcc gcgaagtgat tgctgatgag cgtccagggc gtcgtcgtcc
     7741 aaaccagcaa tgaaaccttg gcatccgccg gcaagcgccg atgcggcaac tcggtccgcg
     7801 tctcgccctc cggcgatcct tctcgatccc tcgatccctc gatcccttga tcccttcgtc
     7861 cctccgcgat cagcgggaac cggacataca ccgacggatc atccaccgtc ctatagccct
     7921 cgcccacctc gccggccgac agcgccgtcc ctccctgcgc ccaccaccac acgactttgt
     7981 gcccctggta cagtaacccg cggtcgaaca gcgtcttcag gctccaccag acgctttcca
     8041 cgtagctctg atgatacgtg acgtacgcct cttccaggtt cacccagaac cccagccggc
     8101 gcgtcatttc ctcccattcg cgcacgtacc ggaacacgct ctcgatgcac ttctgcacga
     8161 agcgcgcgac gccgtaagcc tcaatctcct ccttcgtgtg aattcccagt tgcttgcaga
     8221 cctcaacctc gaccggcaga ccgtgcgtgt cccagccggc cttccgctcc accagcttgc
     8281 cgcacatcgt ctgatagcgc ggaaatagat ccttgatcgc acgcgtcagg cagtgcccgg
     8341 gatgcggcag cccgttggcc gtcggcggac cctcataaaa cacgaaccgc ggcgccccgc
     8401 gtcgcagctc cagcgacttg ccgaagatgt cgtgcttgcg ccaaaactcg agcacttgca
     8461 gctcggcctg cgaaaaattg acctgcgccg gcaacggctc aaacatcgct gctccaaaac
     8521 gctcaaagcc tgtaaataaa tcgaaccact tatgatactg atcgcggcgc aacctgacta
     8581 gccggcgcgg caaccgggta tactcccggc atacgaacac cgggggagcg gaggaaccga
     8641 tggcggcgct ggtcatttac ggcgctgatg gccggcaagg cgtctggcag ctctcgcaga
     8701 agccgctgac gctcggtcga gccgaaaact gcgacatcgt gctgcggttc gacggcgagg
     8761 tttcgcgcga acacgcgcgc gtctggctcg acgagaccgg caccgttctc gtcgccgatc
     8821 tgggctccaa gaacggcacc agcgtcgatg ccggcgagac cttccgcaac gaaacgcgcc
     8881 ccgcgtacag gtccattcgt atcggcggcc aggaagtcac catccgaggc gcgctccggc
     8941 cgccggccag cgcggaaacc cgggtggcct tcgtggcaga cgcccccgag cgcctgactc
     9001 aaagccattt tttcccgtcc acgaaacgcc ttgatctaag cgagcagcgc ctgtcgctgc
     9061 tcatgggctt gggccagtcc ctgtcgggcg ccttcgagcg caagcaactc ctcgagcagg
     9121 ctctggacgc ctgctgccag gccctgcaat tcgagcgctg cctgatcgtc ctgaaaacgc
     9181 cgcgcggcga gcccgaatcg cccgtcgtcc gcaacgtcga gcgcgacgaa accggggcct
     9241 tcaaggtcag tcgcacgctc attaaccgcg ctttgctgca cggtgagcgg gccgtggtca
     9301 acaacccggc cgtcgatctc gtgggcaata tcaccgatag cctggtgcgc ttcccgatct
     9361 gctccgccct cagcgtgccc atcatgcagc gcgatgaaat cctgggcgcc atctacggcg
     9421 atcgcgtgac gcgcgccgcc acttacagca cgcaggacgt ggacttcctg gcggccatcg
     9481 cccagcaagt cggcgtcggt ctggccaacc tgcgcctgct ccaggattac gtccgcctgc
     9541 agaaagtgga cgccgaactc agcgccgccc gcaaaattca acgcgacctg ctgccggccg
     9601 ccccgctggc cgccggctgc gtcctgattg acggctacaa cgagccctcc tccgaggtcg
     9661 gtggtgacta cttcgattac ttcccgctcg atgacacgca cgtcggcttc gtcatcgccg
     9721 acgtcacggg gcatggcctg ccggccgccc tgctaatggc aaactttcag gccgcggtgc
     9781 acgtcgcgct cacgcctgac acgccactgc ccgaaatcgc cgcccggata aaccggctgg
     9841 tgtgccagaa cacggctgga agctccttca ttacggcggt tttgggcaag gttgactgcc
     9901 gcagcggcgc gatggattac gtcaacgccg gacaccccgc tccgctgctg cccggctgcg
     9961 ccaacgcggg cccggatgac cggcataact cgctgccgct gggcatcgat cccggcgaag
    10021 atttcagggt tgagaagctc aatcccgcgg agcacgccgg cgtcgtcctt ttctacaccg
    10081 acggtttgac agagctcaat aatcccgctg gcgacatgct cggtatcgag gcggttcgcg
    10141 acgctctgca aaaactcccg gccgcatcga ctgaactggc gctgcgcgcg gcgctggctt
    10201 tggcgcgcca ccatcgcggc catgccaaag gctcggatga ccttacgctg cttgcaattc
    10261 aggttacccg tccttaagcg gcgctcggtc aggcgggggc gggctcggcc gcccctaccg
    10321 aaatgggcct gtcggccttt atacattttt acaccaccag taatcgcaac cccgccgaaa
    10381 ggggcgtgta gaacctccgt cgcaccgcat gcgcaataat cggccttaac agaccgcgag
    10441 gaaaactatg gttcggcgac agattggctt cgagcccccg gccgggtttg acgaactcga
    10501 taatcccgga tctaacggcg atcgtatgcc agccagtaca ccgacgcccg tgctcttgag
    10561 cctgcttcag caggcggccg cacgccaggc ttccgatatc cacctggttt ccggctatcc
    10621 cgcgacgttc cgcgtgaacg gccggctgga gcgcgccgcc cccaagccgc tgacgtcgga
    10681 tgacatccgg tgcctggctg aatccatcct gccggccgcg gtgttccaga cgttcgagca
    10741 aaccaagaac gcggattgct cgctgtcggt ggagaaaggc ggtgctccgc cgctgcgctt
    10801 tcgcgccaac gtgtacctgg cccgcgacgg ctggtgcatc tgcttgcggc acatccctaa
    10861 cgaaatcccc acgttggact ggctggggtt tcccaaggag ctggccgagc ggctggtggc
    10921 gtaccaggac gggctggtgc tggtgacggg cgaaaccggt tccggcaagt cctcgacgct
    10981 ggccgcgctg atcgatctgg cgcggcggac gcgcgatgcg cacatcatca ctatcgagga
    11041 gccgatcgag tacatccatg aagtgcgtgg cttgagcatc gtcacacagc gcgaggtggg
    11101 gcgcgacgtg agctcgttcg ccgacggcct gaaatacggc ttgcggcagg acccggacat
    11161 catcctggtg ggcgaaattc gcgaccgcga cacggcccag atggccatca gcgcggcgga
    11221 aaccgggcac ctggtgttcg ccaccatgca cacgcgcgac gccaagggag cgctgacgcg
    11281 cttggtagat ctgttcccgc aggattcgca ggacgacatc cgcacgcaat tggcgctgag
    11341 cctgcgttcc gtggtcagcc agaaactgct cccgccggcc gaagaagggc aacggcgcgt
    11401 gctggcgctg gaagtgctgc acgccagcca gccggtacag gtggcgatcc gcagcgggcg
    11461 catcgagacg ctggattcgg cgctgcaaac cggggcgcgc agcggcatga tcatgctgga
    11521 tgacgacctg cggcgcctgg tgcgcgaagg caaaatcacg ac
//
LOCUS       DAOSYS010000101        11560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4751_length_11560_cov_4.976097, whole genome shotgun sequence.
ACCESSION   DAOSYS010000101 DAOSYS010000000
VERSION     DAOSYS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4751_length_11560_cov_4.976097"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLU99_07875"
     CDS             <1..349
                     /locus_tag="PLU99_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17012.1"
                     /translation="LGLARWAHAGLPRVHLVDPPPGKPCTGKAVTLKLDDGLSGRGGW
                     GHDAVKIALDSLRVRVNQTLVTCRPDLKKGTLQVPLPRKLKAGPLRVAVDFENVFGQH
                     VLHPELVLEYAGR"
     gene            complement(502..960)
                     /locus_tag="PLU99_07880"
     CDS             complement(502..960)
                     /locus_tag="PLU99_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17013.1"
                     /translation="MPTTRSYYKSSTPHMGCRRTCRTGTRKVTTFTKNSPAFKNVRDE
                     CQWRMGSYRTVWSQFTGAGQRTPFSPTNANRWLRYVNSGYRVYKWTPKDFYRYFGQQW
                     QQQTPASCFRWMRRKYGACIKDITRGAGNCWLVATSPSVTGYPFQSYRWK"
     gene            complement(1392..3707)
                     /locus_tag="PLU99_07885"
     CDS             complement(1392..3707)
                     /locus_tag="PLU99_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOO17014.1"
                     /translation="MNAQRTMSISPVWLLVAIFTFAAGFFVLIRLAVVMEREKPPVPQ
                     RVIGADGEVLFTGDDIQTGQHVFQKYGLMQYGTLFGHGAYLGPDFTAQYLHRAGQTML
                     DFYGQQHVKPGEAEQRVRSDLKANRYDDATGTLQFNAAQAAAFAEAKEFYKEWFSGQS
                     TQAKLRPPRISDPGELHNLTAYFAWAAWVSAAQRPGSPYSYTNNWPPEPLADNYPTAE
                     ALLWSVLSLIALLGGVGLIMYLFGRYNLLGWHRRDEEEPGRQLEFRPPEEVRLTPSQR
                     ATAWYFLVVAGLFLFQGLLGGLNAHYHVEPEGFYGFDIGRVLPYNLSRMWHVQLALFF
                     VASAFLAMGIFIAPLIARSEPKHQDKLSIALFGALVFVVLGSLLGEGAAIWNWLGLDG
                     PWFWIGIQGWEYLDLGRIWQILLVVGMTLWLVILVRGIWRRLRGEHPGNMPWLFLYSA
                     IAIPLFYAAGLMFGKASEYVTVDYWRFWVVHLWVEDFLELFTTIMVAYIFVLLGVVRT
                     TTATRVIYLDVILYSVGGVIGTMHHLYFSGTPAVHMALGAFFSAMEVIPLVLLTYEAW
                     RFMRLGGVKGELSTLGASPQIFPHKWAVMFLIAVGFWNFIGAGVFGFLINLPFVSYYQ
                     IGTAFTPNHGHGALMGVYGMLAMGFFMFVARYFIPPDRGSNIAMATSFWGLNIGLALM
                     LFVNLIPMGIVQLFDSARNGYWHARDLAFFEQPAVRVIEWLRVPGDVIFIVFGILPVV
                     YLAIRMFRGRNRYGELPPETEAESFVLAYER"
     gene            3918..6056
                     /locus_tag="PLU99_07890"
     CDS             3918..6056
                     /locus_tag="PLU99_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOO17015.1"
                     /translation="MNSGLSLAPGRPGIVPTWTSSAKTGVGAALSGANHCWFTLGHGI
                     FDGIYYPRLDQTCTRDMGLLVTDGRGFFSEEKRHCRHEISRLAPDVPAYRLVNTCNHG
                     RYRMEKRVIADPRRDVILQWTRFTPLQGTLADYHVYVLLAPHLGNEGYGNTAWVDDYK
                     GTPLLLAQRDALGLALACNAGWLKRSAGYVGVSDGWQDVSRHFQMQWTYGRAEDGNVA
                     LIGEVDLQACQGEFLLAVGFGINTAAAAFRARQSLADGFEEAAQAYIQGWEEWLGGVE
                     IVAPPAQHANLWRISAAVVRAHEALAFPGGIIASLSVPWGAARTATNDLGGYHLAWPR
                     DLVMGASGLLALGAGDDVRRSLRYLEGTQEADGHWPQCMWCDGRPYWDGIQMDEAALP
                     ILLADMAWRHKFLEESQRERFWAMVRQAANYIMHNGPITQQDRWENNAGFAPFTIATE
                     IAALLAAADWADMAGERDLAGRWRSTADAWNAQIEDWCYVEATELARRVGVDGYYMRI
                     APAQYHADRGPFAHKEHPLSSVDAVASSVVSPDALALVRFGLRQPHDPRILNTIKVID
                     ALLKVETPYGPCWRRYNGDFYGEYDDGRPFDGNGVGRAWPVLTGERAHYELAAGRPDE
                     ARRLCGAMVAFANEGGMIPEQIWDSPDLPERGLYFGRPSGSAMPLVWAHAEYVKLVRS
                     LSEGRVFDLPPQTAKRYLGQPVPEPAAAMV"
     gene            complement(6118..6930)
                     /locus_tag="PLU99_07895"
     CDS             complement(6118..6930)
                     /locus_tag="PLU99_07895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO17016.1"
                     /translation="MKRTAKRRLAGLAVVAAIVAVSAVVIVPELWRAAAERHLMHLLT
                     STDEEARKQGAWQAVTRSPGPATDFMCAALSQGREPSPGVRESYVYALGRMRLQSALP
                     LLLRLARADESGYVRQAAWIAAARVDFEQFRAAAREVENCADTWDRIGIATALMEADD
                     FSQLDLLFDVAASGDDFQRNVAAAALRRYLRPLLQAAGRWPVNAEISVDGAWSPAFIA
                     EVRGRAASLNLPQIAAHSRPEQQGTETLRRMIRRIYGARERVVRALYSIEVQ"
     gene            complement(7013..8410)
                     /locus_tag="PLU99_07900"
     CDS             complement(7013..8410)
                     /locus_tag="PLU99_07900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO17017.1"
                     /translation="MPDLLFHGAAGEVTGSMHMVEVDGHWVALDCGLFYGRRQEAEDK
                     NRRWPVSPLKIAAVVLSHAHIDHCGRLPRLVKDGFDGPIYCTPATRDLAAIMLPDSAH
                     IQEEDVRYVSKKRRRQGLPPIEPLYTYEDVMATIKLFQTISYKRWFQVIPGLLACFEE
                     AGHMLGSSGVRLEFAKRNGATPSLFYTGDVGRWEQPILRNPADFPPNETIICESTYGG
                     RVNETVDDAKEQLLNVVRHTIDRGGKVIIPAFSVGRTQVIVYYLHELMLAGLLPQIAI
                     FVDSPLAVNATEVFKMHPECYDADARALYQVAGDILGGGYCTYVRTLAQSMALNERTE
                     PCVIISASGMCEAGRIRHHLKHNITNPKNTILLAGYQAANTLGRKLEQGAERIRLFQE
                     EYAVRAEVRQIHGFSGHADQTELLRLLTPRASETRALYLVHGEPDQAQELADRLRAVG
                     FRKVVVAERDRRYSL"
     gene            8671..>9170
                     /locus_tag="PLU99_07905"
     CDS             8671..>9170
                     /locus_tag="PLU99_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOO17018.1"
                     /translation="MRRLRGSAWLVAGALVVTLLPTPALGAKKILRVLLDTRVLEAPA
                     ERFSLSAVLSGEQPKTLLEWERMIRRAADDKEIAGMALIISEPQIQLAQVEELTAALK
                     AFRDKGKKVHCYLEVGGNASYALACAADHITLADMSDLHITGINAMTVFFKGLLDKIG
                     VQADML"
     assembly_gap    9171..9270
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9271..10274
                     /gene="sppA"
                     /locus_tag="PLU99_07910"
     CDS             <9271..10274
                     /gene="sppA"
                     /locus_tag="PLU99_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOO17019.1"
                     /translation="TQLMQLFTKLSETAEAAARPGVAVLYIDGGITMGKSEPSIFDSR
                     NAGSTTIRAAFEKARTNENVKAVVVRVDSPGGSALASDIMWHAARRTAEEKPLIVSMG
                     SVAGSGGYYVSVPGQTIFANASTITGSIGVLGGKLVWHGLMTDKLGITFTEYQRGARA
                     GLMSPQTPWTDSERAFMQKYIEDIYAQFKARVQESRGDRIKGKLEDVAGGRVYTGEQA
                     LKLGLVDKIGGLSDAIRHAATKAGLGDEYQIYIYPEERDIFQELVAQLTGEEKRDEWE
                     LADVSLSFDNPLMRALLPLLQQVAPRQAQAIAAGLRNLLIIDHEHVGCFMPALIEPQ"
     gene            10610..>11560
                     /locus_tag="PLU99_07915"
     CDS             10610..>11560
                     /locus_tag="PLU99_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOO17020.1"
                     /translation="MPSRKRANELLTLLFTCVGRRVELLQAFRAAARRLDQPLRIVAV
                     DSDPTAPALLCADEPHLVPRADDSGYMASLMATVTACRAQALIPTTDTDLQIISAHRA
                     ELTAAGCTALIADPPVIEICRNKLRTYDFLRQHGFDTPATFTPAQIRAGEARPAFPLF
                     CKPLSGSASRRVNVVRDEFELEYVLRRHSDLIVQELVVGQEYTLDVYVGLEGVPRCVV
                     PRARWQVRSGEVSKGVVVKDPEIMNAGKRVVEALGKSLRGLVNLQCIVTPERRIRFIE
                     INPRFGGGAPMAIAAGADYPSWLIQELRGERPEIAFGGFRH"
BASE COUNT         2128 a         3590 c         3732 g         2010 t
ORIGIN      
        1 cctcggcctg gcgcgctggg cgcacgccgg cctgccgcgc gtccacctgg tggatccgcc
       61 gcccggcaaa ccgtgcaccg gtaaggccgt tacgctgaag ctggacgatg ggctgagtgg
      121 ccgcggcgga tggggccacg atgcggtcaa aatcgctttg gattcgctgc gggtacgcgt
      181 gaatcagacg ttggtcacgt gccggccgga cctgaagaag gggaccctcc aggttccgct
      241 gccgcgcaag ctgaaagcgg gtccgctgcg ggtggcggtt gacttcgaga acgtgttcgg
      301 ccagcacgtg ctgcatcccg agctggtgct ggagtacgcg ggccgctaag cgggcgcgac
      361 gcgccgcctc cgcgccagga ttggcctgtg ccaacgaaag cggcccgccg ctttgcgcga
      421 cgggccgcac aattcgtgca gtgcagcagg caaggtccgg gaaggccgcc cacccgtggc
      481 gggcggccgg gtttagcgta actacttcca gcgatagctc tggaacggat agccggtgac
      541 gctcgggctg gtggcgacca gccagcagtt cccggcgccg cgcgtgatgt ccttgatgca
      601 cgcgccgtac tttcgtcgca tccagcggaa gcaactggcc ggggtttgct gttgccactg
      661 ctggccgaag tagcgataga agtcttttgg cgtccactta tagacgcgat agccgctgtt
      721 tacgtagcgc agccatctgt tcgcgttggt gggggagaag ggcgtgcgct ggccggcgcc
      781 tgtaaactgc gaccagacgg tacggtagga gcccatgcgc cactggcact catcgcgaac
      841 gttcttgaag gcgggggagt tcttggtgaa cgtggtcacc ttgcgggtgc ccgttcggca
      901 ggtgcgccgg cagcccatgt ggggcgtgct cgacttgtaa tagcttctgg ttgtcggcat
      961 agttctctcc atgtgcctgc tgattgcgtt gccctcaatt cgggaggggt taatggtttt
     1021 tgtatcggga ccatgtcggc agaagttaaa taacatcttt aagtttggct tgggcggcct
     1081 cgcctgcggg tcgcgccgcg ggggtagcgc gccgcgcggt tcttcgggcc tggcccggtg
     1141 accagcatgg acgctgagcc ggcagggctg acggcagttg ttctcggaac ttgcgcggcg
     1201 taagcgtccg ccgggccggc acggtagccg gtcgcggttt acacgtccgt tggccgacgt
     1261 ttcgccggcc tggaaggccc cccggcctgc ctggtgcccg gcagattgct atgtgcgccg
     1321 ccgcgccccg agcgaacccg catctccccg caacctttgc gcgatacgga accgtgccgg
     1381 gcgagcttca atcaccgctc atacgccagg acaaacgact ccgcctccgt ctcgggcggc
     1441 agctcgccgt aacggttgcg gccacggaac atgcggatcg ccaggtacac caccggcagg
     1501 atgccgaaaa ctatgaagat cacgtcgccc ggaacgcgca gccattcgat cacgcgcacc
     1561 gccggctgct cgaagaacgc caggtcacgc gcatgccagt acccgttgcg cgcggaatcg
     1621 aagagctgca cgattcccat cgggatcagg ttgacgaaca gcatcagcgc cagcccgatg
     1681 ttgaggcccc agaacgaggt cgccatggcg atgttgctgc cacggtccgg cgggatgaag
     1741 taacgcgcca cgaacatgaa gaagcccatt gccaacatgc cgtagacgcc catcagcgcg
     1801 ccgtgcccat gattgggcgt aaaagccgtg ccgatctggt aatagctgac aaacggcaga
     1861 ttgatcagga acccgaacac gccggccccg atgaaattcc agaagccgac cgcaatcagg
     1921 aacatgaccg cccacttgtg cgggaatatt tgcggtgacg cgccgagggt tgatagttcg
     1981 cccttcacgc cgcccaggcg catgaaacgc cacgcctcat atgtgagcaa taccagcggg
     2041 attacctcca tggccgagaa gaaggccccc aacgccatgt gtaccgcggg cgtgccgctg
     2101 aaatacaggt ggtgcatcgt gccaatgacg ccgccaacgg agtacagaat cacgtccagg
     2161 taaataaccc gcgtggccgt cgtcgtgcgg actacgccca gcagcacgaa aatgtacgcc
     2221 accatgatgg tcgtgaagag ctcgaggaag tcctcgaccc acaggtgtac gacccagaag
     2281 cgccagtagt cgaccgtcac atattcagaa gccttcccga acatgagccc ggccgcgtag
     2341 aacagtggaa tagcaatcgc actataaagg aacagccacg gcatattacc ggggtgctcg
     2401 ccgcgcagcc ggcgccagat gccgcggacc agaattacca gccacagcgt catgccgacg
     2461 accagcagaa tctgccagat ccgccccaga tccaagtact cccagccctg aatgccgatc
     2521 cagaaccacg gcccgtccaa ccccagccaa ttccagatgg cggccccttc gcccagcagg
     2581 ctgcccagta cgacgaacac cagcgcgccg aatagcgcaa tcgacagctt gtcctggtgc
     2641 ttaggctcgc tccgcgcgat cagcggcgca atgaaaattc ccatcgccag aaacgccgaa
     2701 gccacaaaga acagcgccag ttgcacgtgc cacattcgtg acaggttgta aggcagcacg
     2761 cgcccaatat cgaagccgta gaaaccctcc ggctcgacgt ggtaatgcgc gttaagcccg
     2821 cccaataacc cctgaaacag aaacagtccc gcaacgacca aaaaatacca tgccgtggcc
     2881 cgttgcgacg gcgtcagccg cacctcttcc ggcgggcgaa actccagttg ccggccgggc
     2941 tcctcctcgt cgcggcgatg ccagcccagc aggttgtagc gcccgaatag atacatgatc
     3001 agtcccacgc cgcccagcag cgcgatcaag ctcaacacgc tccacagcag ggcttcggcc
     3061 gtcgggtaat tgtcggccaa cggctccggc ggccagttgt ttgtgtacga gtaggggctt
     3121 cccggacgtt gtgcggctga cacccacgcc gcccacgcaa agtaagcagt gaggttgtgt
     3181 aactcacccg ggtcggatat gcggggaggt ctcagcttcg cctgcgtcga ctgcccactg
     3241 aaccattcct tgtagaactc ctttgcctcg gcgaaggccg cggcctgggc ggcattgaac
     3301 tgtagcgttc ccgtcgcgtc atcgtaacgg ttcgccttca ggtcggaacg cacccgctgc
     3361 tcggcctcgc cgggcttgac gtgctgttgg ccatagaagt cgagcattgt ttgcccagca
     3421 cggtgcaggt attgagcggt gaaatcgggc ccaagatacg ccccgtggcc aaagagcgtt
     3481 ccgtactgca tcaggccgta tttctgaaat acgtgctgtc cagtctgaat atcgtcgcct
     3541 gtgaacagta cttccccatc cgccccgatg acgcgctgcg gaacaggcgg cttttcacgc
     3601 tccatgacga ccgccagccg gatgagaacg aagaagccag cggcgaaagt aaagatcgcc
     3661 accagcagcc agaccggcga aatgctcatt gtgcgctgcg cgttcacgtt ggcctccgga
     3721 tgcttctcat agaaaaggac cgctactcgt gctgcgtaac gcgcgtgccg tgcctcactg
     3781 gattttgtgc tttacgcgca cgacatccga catgataccg catttctgac aaaccaccca
     3841 tggccgcaat aatccaaaga tggcgggagc ctgttgcagc atcacgggcc cgccgctctt
     3901 acgagaggaa acagattatg aactctggct tgtcgcttgc tcccggccgg ccgggaatcg
     3961 tgcccacctg gacttccagt gccaagacgg gcgtgggcgc agcgctgtca ggcgcgaatc
     4021 attgctggtt tacgctgggc catggcatct ttgacggcat ttattatccc cgcctggatc
     4081 agacctgtac gcgcgacatg ggcctgctcg tgacggacgg ccgcgggttc ttctccgagg
     4141 aaaagcgaca ctgccgacac gaaatctcgc gacttgcgcc ggacgtgccc gcttaccgcc
     4201 tggtcaacac ctgcaatcac gggcgttacc ggatggaaaa gcgcgtcatc gcggacccac
     4261 gccgcgacgt cattctgcaa tggacccggt tcactccgct gcagggcacg ctggccgatt
     4321 atcacgttta cgtcctgctc gcgccgcatc tgggcaatga aggctatgga aacaccgcct
     4381 gggtcgatga ctacaagggc acgccgctgc tgcttgcgca gcgcgacgcg ctcggccttg
     4441 ccctggcgtg caacgccggc tggctcaagc gctccgccgg ctacgtcggc gtctccgatg
     4501 gctggcagga tgtgtcgcgc cacttccaaa tgcagtggac ctacggccgc gccgaagacg
     4561 gcaacgtggc cctgattggc gaagtggact tgcaggcgtg ccagggcgag ttcctgctag
     4621 cggtcggctt cggcataaat accgcggccg ccgcgttccg cgcacgtcag agcctggccg
     4681 acggcttcga agaggcggct caggcttaca tccaggggtg ggaggaatgg ctcggcgggg
     4741 tcgaaattgt cgcgccgccc gcacagcacg ccaatctatg gcggatcagt gcggcggtcg
     4801 tgcgtgcaca cgaggcgctg gcgtttccgg gcgggatcat tgcgagtctg tcggtcccct
     4861 ggggcgccgc gcggaccgcc accaacgacc tgggcgggta tcacctggcc tggccgcgcg
     4921 acctggtgat gggcgcgagc gggttgttgg ccctcggcgc aggggatgac gtgcgccgca
     4981 gcctgcgtta cctggagggt acgcaggagg ctgatgggca ctggccgcag tgcatgtggt
     5041 gcgatgggcg gccgtactgg gacggcatcc agatggacga ggcggccctg ccaatactgc
     5101 tggccgacat ggcgtggcgg cacaagtttc tcgaggaatc gcagcgtgag cgcttctggg
     5161 ccatggtgcg acaggcggcc aactacatca tgcacaacgg ccccatcacg cagcaggatc
     5221 gctgggagaa caatgctgga ttcgcgccgt tcacaatcgc cacggaaatc gccgccttgc
     5281 tcgcggccgc ggactgggcg gatatggccg gcgagcggga cctggcgggg cgctggcgga
     5341 gcacagccga cgcctggaac gcgcagatcg aagactggtg ttacgttgaa gcgacggagc
     5401 tggcgcggcg cgtcggcgtg gacggctact acatgcggat cgcgccggca caataccacg
     5461 ccgaccgcgg cccctttgcg cacaaggagc acccgctgag ttcggtggac gcggtcgcca
     5521 gcagcgtagt cagtcccgat gcgctggcgc tggtgcgctt cgggctccgc cagccgcacg
     5581 atccgcgcat cctcaacacc atcaaggtca tcgacgcgct gctgaaggtc gagacgccgt
     5641 acgggccgtg ctggcggcgc tacaacggcg acttctacgg cgaatacgac gacggccggc
     5701 cgttcgatgg caacggcgtg ggccgggcct ggccggtgct gaccggcgag cgcgcccact
     5761 acgagctggc ggccggtcgc ccggacgagg cgcggcggct gtgcggggcg atggtggcct
     5821 tcgccaacga aggcggcatg atccctgagc aaatctggga cagccccgat ctcccggagc
     5881 gcgggctata cttcggccgg ccgtcgggct cggccatgcc gctggtgtgg gcgcatgcgg
     5941 agtacgtgaa gctggtgcga tcgctgagtg aggggcgggt cttcgacctt ccgccgcaga
     6001 cggccaagcg ctatctgggc caacccgtgc cggagcccgc ggcggcgatg gtatgagaag
     6061 agccggcagc gttcggctat gaggtctcgc cttgggggcg gcgaggagtg gcggcgccta
     6121 ttgcacctcg atcgaataca gtgcgcgcac cacgcgctct cgtgcgccat aaatccggcg
     6181 gatcattcgc cgcaaagttt cggtaccctg ctgctcgggc cgtgaatgag ctgcgatttg
     6241 cggcaggttc agcgatgcgg cgcggccgcg gacttcggca atgaaggctg ggctccaggc
     6301 gccgtcgaca gagatctcgg cgttcaccgg ccagcggccg gcggcctgca atagcgggcg
     6361 cagatagcgg cgcaaggctg ccgcagcgac attgcgctgg aagtcgtcgc cgctggcggc
     6421 cacgtcgaat agcaggtcta attgcgaaaa gtcgtccgcc tccattaaag cggtcgctat
     6481 gccgatgcga tcccaggtgt cggcgcaatt ctccacttca cgtgcggccg ctcgaaactg
     6541 ctcgaagtcg acgcgcgcag cggcgatcca ggcggcctga cgcacatatc cgctctcatc
     6601 cgcgcgggct aggcgaagaa gcagcggcag agcgctttgc agacgcattc ggcccagggc
     6661 gtagacgtaa ctctcgcgca caccggggct gggctcgcga ccctgcgata gcgcggcaca
     6721 catgaagtca gtcgccgggc cgggcgagcg ggtcacagcc tgccaagccc cttgtttgcg
     6781 cgcctcttcg tcagttgagg tgagcaagtg catgaggtgc cgttcagccg cggctcgcca
     6841 tagttccggt acgatgacaa ctgcggacac cgccactatt gcagctacca cggccagccc
     6901 ggcgagcctt cgtttggctg ttcttttcat gggcgtaacc agggctctgt tcgctgttgc
     6961 gccggcccga agttttcggg ccccaaaaac cgcgggccga caccggcaaa tcttacaggc
     7021 tgtagcgccg gtcgcgctcg gccactacca ctttacggaa acccaccgca cggagtctat
     7081 cggccaactc ctgggcctgg tccggttcgc cgtgcaccag gtacaaggcg cgcgtttcgc
     7141 tggcgcgcgg tgtgagcagc cgcaacaatt cagtctggtc ggcatgaccg ctgaagccgt
     7201 gaatctgccg cacttcggcg cgcaccgcgt actcctcttg gaaaagccgg atgcgttcgg
     7261 ctccttgttc gagtttgcgg cccagcgtat tggcggcctg gtagccggcc aggaggatcg
     7321 tgttcttcgg gttggtaatg ttgtgcttaa ggtggtgccg gatgcggccg gcttcgcaca
     7381 tgccgctggc ggaaattatg acgcacggtt cggtgcgctc gttgagcgcc atgctctgag
     7441 ccagcgtgcg gacgtacgtg cagtagccgc ctcccaggat gtcgccggcg acctggtaca
     7501 acgcgcgggc atcggcgtcg tagcattccg ggtgcatctt gaacacttcg gtcgcgttca
     7561 ccgccagtgg gctgtccacg aagatcgcaa tttgcggcaa cagcccggcc aacatcagct
     7621 catgcaggta atagacgatt acctgcgtgc ggccgacaga gaacgccggg ataatgacct
     7681 tgccgccgcg gtctatcgtg tggcggacca cgtttagcag ttgttccttc gcatcgtcca
     7741 cggtttcgtt gacgcgtccg ccgtacgtgc tttcgcagat gatcgtctca ttgggcggga
     7801 agtcggccgg gttgcgcaga atgggctgct cccagcgccc cacgtcgcca gtgtagaaga
     7861 gcgagggggt ggcgccgttg cgttttgcga actccaggcg cacgccggac gagccgagca
     7921 tgtgtcccgc ctcctcgaag caggccaaca gcccgggtat gacctggaac cagcgcttgt
     7981 acgagatggt ctggaagagc ttgatggtcg ccattacgtc ttcatacgtg tacagcggct
     8041 cgatgggcgg caggccctgg cgccgccgtt tcttgctcac gtagcgaaca tcttcctcct
     8101 gaatgtgggc cgaatcgggc agcatgatgg ccgccaaatc gcgggtggcc ggcgtgcagt
     8161 agatcggccc gtcgaagccg tccttcacca gccgcggcag gcgcccgcag tgatcgatgt
     8221 gggcgtgcga gagcacgacg gccgcgattt tcaggggtga gaccggccag cggcggtttt
     8281 tgtcctcggc ctcctggcgg cgtccataga acaggccgca atccagcgcc acccagtggc
     8341 catcgacctc gaccatgtgc atcgagcctg taacttcgcc cgctgcgccg tgaaacagca
     8401 aatccggcat ttcggacctc ctgattgcaa cggaaatggt aaggctgaag gtagcggcga
     8461 gcaaaacgga agtgataggt tgccgtgccg tgattatgcg gcgaacggcg ggagaatgtg
     8521 cgacgaaaaa gctccgacaa gttctgacgt acgggaaaac gtcgcgcctt attatcatgg
     8581 cgccgtgtgt gaggtcgtgt ctggacatta tgggtcaagg agtcgcggct aaagcggcgc
     8641 gccgccgctg agaccgctgg gaggttcttc atgaggcgat tgcgtggaag tgcatggttg
     8701 gtggcaggcg cgttggtcgt gacgctgttg ccgacgcccg ccctgggcgc gaaaaaaatc
     8761 ttgcgcgtgt tgctggatac gagagtgctc gaagcgccgg ccgagcgctt cagcctgagc
     8821 gcagtgcttt cgggcgagca gcccaagacg ctgctcgagt gggaacgcat gattcgccgc
     8881 gccgccgacg acaaagagat cgccgggatg gccctgatca tctcagagcc gcagatccaa
     8941 ctggctcagg ttgaggaact gacggcggcg ttaaaagcct tccgcgacaa gggcaaaaag
     9001 gttcactgct acctggaagt gggcggcaac gcctcgtacg ccctggcttg tgccgcggat
     9061 cacataacgc tggcggacat gagcgatctg cacatcaccg gcataaacgc catgacggtg
     9121 tttttcaagg gtttgctgga caagatcggc gttcaagccg acatgctgca nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacgcagtt gatgcagttg ttcacaaagc
     9301 tgagtgagac ggccgaggct gctgcgcggc cgggcgtggc cgtcctgtac atcgacggcg
     9361 gtatcaccat gggcaagagc gagccgagta ttttcgactc gcggaatgcg ggcagcacga
     9421 cgattcgcgc ggccttcgaa aaggcccgca cgaacgagaa cgtcaaggcg gtcgtcgtgc
     9481 gcgtcgattc gccgggcgga tcggcgctgg ccagcgatat catgtggcat gcggcgcgtc
     9541 gcacggctga agagaagccg ttgatcgtga gcatgggttc cgtggccggc agcggtgggt
     9601 actacgtctc ggtgccgggg cagacgatct tcgcgaatgc gtcgacgatc accggttcga
     9661 tcggcgtgct cggcgggaag ctcgtttggc acggactgat gaccgacaaa ctgggcatca
     9721 cctttactga atatcagcgc ggggcgcgtg ccggcctgat gagcccgcaa acgccctgga
     9781 ctgactccga gcgggcgttc atgcagaagt acattgagga catctacgcc caattcaagg
     9841 cgcgcgtgca ggaatcgcgc ggcgatcgga tcaaaggcaa actcgaggac gtcgccggcg
     9901 ggcgcgtgta taccggcgag caggccctga agctcggcct ggtggacaaa atcggcggtc
     9961 tgtccgacgc gattcgccac gcggccacga aggccgggct cggcgacgaa taccaaattt
    10021 acatctatcc cgaagagcgg gacatcttcc aggagctggt ggcgcaactt acgggcgaag
    10081 aaaagcgcga cgaatgggaa ctcgccgacg tgtcgctctc gttcgacaac ccgctgatgc
    10141 gcgccctctt gccgctgctc cagcaggtcg cgccccggca ggcccaggcc atcgcggccg
    10201 ggctacgtaa cctgctgatc atcgaccacg agcacgtcgg ctgcttcatg ccggcgctca
    10261 ttgagccgca atgaccagcc ggcgacgacg gcccagcaag acgcgcatgg agcaataaat
    10321 ctgcggcccg ggggcgttta cggggttgcg cacggcctgc gcgcataaac gcgcgaagcg
    10381 acaacaccgg ttcacggccc cgtcctcgat gaccttcgca tagcagcgca catcctgcct
    10441 aagggagtga agaaaggggg acaccttccc acgttcccac gttcccacgt tcccacattc
    10501 ccacattccc acaggcagac gtgcgcggcc gggcgaaaac ccctcgcccg ccccgccggt
    10561 ccaggattgc cacttggcgc gcagggatgt ttccgacgat gctatgagaa tgccgtctcg
    10621 gaaaagggct aatgagctgc tgacgctgct cttcacctgc gttggccggc gcgtagagct
    10681 gcttcaagct tttcgcgccg cggcgcgccg cctggatcag ccgttacgga ttgtggcggt
    10741 ggacagcgat cccacggcgc ccgcgttgtt atgcgcggac gagccgcacc tcgtgccgcg
    10801 cgcggatgat tcgggctaca tggcctcgct gatggcaacc gtgacggcct gccgcgccca
    10861 ggccctgatt ccgacgacgg acacagatct gcagataatc agcgcccatc gggccgagtt
    10921 gactgcggcc ggctgcaccg cgctgatcgc cgatccgccg gtaatcgaga tctgccgtaa
    10981 taagctgcgc acctacgact tcctgcggca gcatggcttc gacacgccgg ccaccttcac
    11041 gcccgcgcaa atccgggcgg gcgaggcacg ccccgcgttc ccgctgtttt gcaagccgct
    11101 gagcggcagc gccagccggc gggtcaacgt ggtccgcgat gaatttgaac tcgagtatgt
    11161 cctgcgccgc cacagcgatt taatcgttca ggaactggtc gtcggacagg aatacacgct
    11221 ggacgtgtac gtgggcctgg agggcgtgcc gcgctgcgtg gtgccgcgcg cccgctggca
    11281 ggtgcgcagc ggtgaagttt ccaagggcgt agtggttaaa gaccccgaga tcatgaacgc
    11341 cggcaaacgc gtggtcgagg cgctcggaaa atccctgcgc gggctggtca acttacagtg
    11401 catcgttacg cccgagcggc gcattcgctt catcgaaatc aatccgcgct tcggcggcgg
    11461 ggcgccgatg gccatcgccg cgggcgctga ttacccaagc tggctgatac aggagctgcg
    11521 cggggagcgg cccgaaatcg ccttcggcgg attccggcac
//
LOCUS       DAOSYS010000102        11555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4752_length_11555_cov_5.470870, whole genome shotgun sequence.
ACCESSION   DAOSYS010000102 DAOSYS010000000
VERSION     DAOSYS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_11555_cov_5.470870"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..516)
                     /locus_tag="PLU99_07920"
     CDS             complement(202..516)
                     /locus_tag="PLU99_07920"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOO17021.1"
                     /translation="MENFVSEPISPHRGTFMARPMAGGLPGLPGGFDWRGKSYEIREV
                     LETWKQSGAEHGRAQGEIYLRRHYFKIKMSDAAVWTVYFTRQAARGGPAKRRWFLYSI
                     DA"
     gene            complement(563..925)
                     /locus_tag="PLU99_07925"
     CDS             complement(563..925)
                     /locus_tag="PLU99_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17022.1"
                     /translation="MGFLSLGALALLGGCTHVIGNNCPYYKQGPAQREGPQGTIPAGT
                     RVWVLGKEGSYARVWAANGVDGYVWDSSIVTVEEWNRRQELDKKNKEAERRARAKGDG
                     PLGPPESYASPPASGSRP"
     gene            complement(1331..2251)
                     /locus_tag="PLU99_07930"
     CDS             complement(1331..2251)
                     /locus_tag="PLU99_07930"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOO17023.1"
                     /translation="MSDRVKQILGWYESDNPGSLANLRRMLGTGTLADTGKMVILPVD
                     QGFEHGPARSFAPNPPAYNPHYHFELALEAGCNAYAAPLGFIEAGARTFAGELPLILK
                     LNNHELLHDERNPMPAITASVEHALRLGCAAIGFTVYPGSALNNGMYEQFQALSEEAK
                     AAGLAVVIWSYPRGENLSKEGETAIDVCAYAAQIACQLGAHVVKIKFPSAHLEQEAAR
                     KVYEKEKVAIAQPAERIRHIVQSAFDGRRIVIFSGGAMKGEAGLFEEVKAIRDGGGFG
                     SIIGRNAFQRPRKDAVAMLKKIMNIYAGKE"
     gene            complement(2820..3554)
                     /locus_tag="PLU99_07935"
     CDS             complement(2820..3554)
                     /locus_tag="PLU99_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO17024.1"
                     /translation="MFAMVANAADLTLFFGRPQTVAATADFVPDSTLPYDQPALPGTY
                     TVGIWAQVALNEAGEALDVWNGIGLKIVGDDGVVISDLLMDNFNHQQGLGVQRRWETG
                     SDFGPNKGQPNDFLLVAVSQGFGLGGWFPRELGLPTTDWWSYGTETPFNYWLGNVTFS
                     YDGSDGPKNVFFAIMGEASGVHEGGVSRQGGDVEVDRIYFGLDEPIGLRGDDWGSASS
                     LPDFTFAPEPASLILLSLAGLFLRRR"
     gene            complement(3900..5918)
                     /locus_tag="PLU99_07940"
     CDS             complement(3900..5918)
                     /locus_tag="PLU99_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO17025.1"
                     /translation="MTPRNRKRPALVKKIPPAQPPTRPRRRLWPFALAAGALLVIVAA
                     ALFRYLHPNAGGTVIGTGLNVLLITLDTTRADFLGCYGRPNGRTPNIDRLAGEGVLFE
                     RCMAPAPLTLPSHASLMTGNSPYVHNARSNGRERLPEANVTLAEVLKQAGYAAQATVA
                     TMVLNRQFGIAQGFDVYHDVSALTPGDPERAQRKGDEVAADAIEMLRSLAGDPFFLWV
                     HFYDPHTPYETTRTPPYTPAEAYEDEIAFMDEQVGRVLAELRSLGLDQRTLVVLAGDH
                     GEGLGEHGESEHGYFLYHTTLHVPLMIRCPWKIAPGTSVADVVRVIDIAPTILALLGL
                     PPLENAEGESLLPWISGQRTGDLFAYSETLEPRDAFGLSHLRAATTREWKYILAPTPE
                     LYDLSADPAELHSLATEQPEVAENMRQELRELLAQAPPAFRDTGPAPALSAADRARLE
                     SLGYAGTQVREESDGPEIERFEPQGGNPADYAAYIELTQQARQAINRGEYQAAEEMLR
                     AVVESLQNSTQVCELQAMALIFQRRPQEALPVLKRALALAPDDRGLRGTYASALNEAG
                     RYQEAAEQFRIVLSDNAQDPRMLHNMAISLTRLGRFAEADQHLQLALNIEPRNARLLH
                     TLGVLRSHQRRFAEAERYLVQALEIDPTLRQCEEDLRRLREARSRLKP"
     gene            complement(6302..7063)
                     /locus_tag="PLU99_07945"
     CDS             complement(6302..7063)
                     /locus_tag="PLU99_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO17026.1"
                     /translation="MRKFLVAFAVLGMFAMVANANDITFYFGRPQTVVATTDFVADAA
                     LPVAQPADPGTYTLGIWAQVNYYQPGETWDAWNGMHINILADEGVVVSGLAIDNFDHR
                     PGAGTAFRWEDGSDFGPGGSPTDFYLVAVTRFGLGGLWARELTGGAVSGTDWWAYCAD
                     PDASGPQQYWLGNVTLSYDGTDAKNVYFQIGTGGIARRGGVPAEDLIYFGLDEPVGLF
                     GYQFGAISSLPDFTFTPEPASLILLSLAGLFLRRR"
     gene            complement(7833..8474)
                     /locus_tag="PLU99_07950"
     CDS             complement(7833..8474)
                     /locus_tag="PLU99_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17027.1"
                     /translation="MRTTILSICLMAAVAGALAAPPHIPTTPAPVEDIVYARPFTLEQ
                     GYRYIFSKERPWVTEGTLLVLKVNKDFVYPREIAMPVLYVGNQPAERLNRGHESGHVI
                     VIVPGRVDLPNTPIWFGAPNFPHMVDAATANAQRELAERAEIKPFSREKVNAALAEGG
                     PRINAADQRTLLRDVVSKLILKYSPQEKHLADDYCVPTVGEPATPPAAPVDEP"
     gene            complement(8530..>11555)
                     /locus_tag="PLU99_07955"
     CDS             complement(8530..>11555)
                     /locus_tag="PLU99_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17028.1"
                     /translation="AFAPGDLNCDGAVNAFDIDPFVLALTNPDGYAASFPLCDVLLAD
                     ANGDGLVNAFDIDPFVEIVVGGGQGEIVPVQLAGNSLTLYPYFEYVKAFNQNATVELA
                     IDPTQHPDIVGRTADVYIVEHKSAGLWAFDPELIDMTADGPLTVTFGGETIQDNTFVI
                     SGPNELNAAVFDPETGDFTGLGAPYDMVVDFDQDGQLSGGDYIDGLGREAGLYVVHDT
                     TQPGPLAVTDVPSYSVGPVFGIPGSYTNEVLFYPSDIASMPPRPLIVVGHGGGHNYQW
                     YSHIGHHMASYGYIVMSHMNCYGPPDCTLGHTDAILDKQDEIAGGVLNGKIDASRIIW
                     IGHSLGGMGVVGAFDALMDGEYTPTHYTPDSIVLISSMLPNAMGGPAEATPHHANFHL
                     WTAAGDTDISGAPYDEQLQTFLQHDRATKYRMSTVLQGVGHAWLHDGPAEPSFFDGPC
                     ALNKTITHRIQLGLFLPLIKYFAEGNVPASDFLWRQYERFHPIGVDTSNPCVVVSHEY
                     RNGADKGNFFIDDYQTGPGLNVSSSGGSVTYTVHNVVEGNLDDANSDFTWMSGDPFNG
                     ATHASSGDTSRGVVFDWNGENVYYEWEVVPAARNFAAYTYLSFRGAQCTRHPYTTAVL
                     GDLTFTVTLRDAAGTTSSINIGAYGGGFEEPYQRTGAGSGAGWHDDFEVIRIRPTDFL
                     TNGTGLDLANIVAVRFNFGPAWGSNEGRIVLDELMLTNDYPAYFVPMSMALPGAAPEF
                     VPPHVPTHINVEIFEGDDSVVAGSPTIHYRYDGGAWLSAPLERIAGELWRGTLPAPEC
                     GDAPEYYFTAQGDVSGTFYLPPGGSGNPYVSYVGNYIPILDDDFEADSGWSVQSQAGM
                     TSGIWQRAIPGGWADNSPPHDFDGSGRCYVTDNRPTYDVDGGPTHLISPLLDLSGTGN
                     PVMRFANWFTCDDPLPPAQDFLDVFVSSDDGATWVQAAHLASHTDWELRQVYFADFIP
                     LTATVRVRFTTVDSPNNSLTEAGVDRVEIFDVQCD"
BASE COUNT         2153 a         3807 c         3499 g         2096 t
ORIGIN      
        1 attgaaacgt ctggataaaa acgccgttac cagacgcttc caatgcccac gtttgctaat
       61 tctaggtgca ttttcacgcc gcgtccagcg cggtttgagt cagtcgcatt gtaactgcgc
      121 gttcagacgc tctggtgtac tgggtcctat atgcaaaggc tccggcaagg gcgcaacgag
      181 ccatcgcccc ctcaccggcg gtcatgcgtc gattgagtat aggaaccagc ggcgcttggc
      241 cgggccgccg cgcgccgcct ggcgcgtgaa ataaaccgtc cagaccgccg cgtcggacat
      301 ctttatctta aagtagtgac gtcgcagata aatctcgccc tgggcccgcc cgtgctccgc
      361 cccggactgc ttccacgtct ccaaaacctc ccgaatctca taactcttcc cgcgccaatc
      421 aaaaccgccc ggcaatccgg gcagtccgcc ggccatcgga cgcgccataa aagtgccgcg
      481 atgcggcgaa atcggctcgc ttacgaagtt ctccacgccc gcattttagc cgcggcacgg
      541 cccaaaactc acgcgccacg ggctaaggcc ggctcccgct cgcgggcggc gaggcgtagc
      601 tctctggcgg tcccagcggg ccgtcgccct tggcccgggc gcggcgctcc gcctccttgt
      661 tcttcttgtc cagctcctgg cggcgattcc actcttcgac cgtcacgatc gagctgtccc
      721 aaacgtaccc gtccacaccg ttggccgccc acacgcgggc gtagctgccc tctttaccga
      781 gcacccaaac gcgcgtgccg gcgggaatcg tgccctgcgg accttcacgc tgcgccggcc
      841 cctgcttgta gtacgggcag ttgttgccga tcacatgcgt gcagccaccc agcagcgcca
      901 aagcgcccag cgataaaaat cccacccccg gccgccgcag ccaagcccga aatccccacg
      961 ccggtcgccg cagcaaagcc cgaattcccg cccccggccg ccgtaataag gccgaaccgt
     1021 taagcatcgc taccctcaca gcattcgcag cagctcgcat cggcggaaat gatactaaac
     1081 cgggtcggct gcgtcagctt cgcaaggaga gcagagccgg ggcgttcgtc gcgcctgatc
     1141 cgagcccgaa gcgctagcga aagcaaggga tatcgaggga ccaagaagag cagagccggg
     1201 gcgtgccgcc tcggaatagc gctaagaatc ccccatccgc gtggtaaacg aatcgcgccg
     1261 caactgctca cactcgcggc gcgacccgcc tggatgaaca atttcacggt gcccgcgtgg
     1321 ctgtttgaac ctattccttg cccgcgtaga tgttcatgat cttcttgagc atcgcgaccg
     1381 catccttcct tggccgctgg aaggcgttgc ggccgatgat cgaaccgaag ccgccgccgt
     1441 cgcgaatggc cttcacttcc tcgaagagcc cggcttcacc tttcatggcg ccgcccgaga
     1501 agatcacgat gcgccgcccg tcgaacgccg actggacgat gtggcggatg cgctcggcgg
     1561 gttgggcgat ggcgactttc tctttctcgt acaccttgcg ggcggcctcc tgctccaggt
     1621 gcgcgctggg gaacttgatt ttgaccacgt gcgccccaag ctggcacgcg atctgggccg
     1681 cgtaggcaca aacgtcgatg gccgtttccc cttctttgct caggttctcg ccgcgcggat
     1741 aggaccagat cacgaccgcc aggccggccg ccttggcctc ctccgagagg gcctggaact
     1801 gctcgtacat gccgttgttc agggccgagc cggggtagac cgtgaagccg atggccgcac
     1861 atccgaggcg cagggcgtgc tcgaccgagg cggtgatggc cggcatcggg ttgcgctcat
     1921 cgtgcagcaa ttcgtggttg ttaagcttca ggatcagcgg cagctcgccg gcgaaggtgc
     1981 gggccccggc ctcgatgaag cccagcgggg cggcgtaggc gttgcagccg gcctccaggg
     2041 ccaattcaaa gtgatagtgc gggttgtacg cgggcggatt tggcgcgaag ctgcgcgccg
     2101 ggccgtgttc gaaaccctgg tcgaccggca ggatcaccat tttgccggta tcggccaacg
     2161 tacccgtccc cagcatgcgc cggagattgg ccaggctgcc cggattgtct gattcgtacc
     2221 accccagaat ttgtttcacc cgatctgaca tcatgacgca tctcccttgt tttgagtgcg
     2281 cgcgccgacc actcgcgata cgaaacaccg cagcgcccgc tccctacggg cgaccgccaa
     2341 gcatggcgcg tcgaacatcc gatgttacga gcaacagcac ggccccgcaa ctcgcggggg
     2401 cgccagcgcg ccagcccgat agccgccaat tgcgcgagtc cgcctccgaa gtcctgcctg
     2461 gccaccaccc gtacgcccca aaagcgtttg ccgcacgaac accctttcat ccaaaccgga
     2521 gcatgccgcc caagccaccg ctgcaagttg agggcccggg ctcgatgacg ggcgccggga
     2581 cgcagctcgc cgcatggtca cgcgcagcgg gaaactgaat tggtagctgg cgtgcggccg
     2641 tttgacggcc gcgccgcggc tctatcacgc tttttcctta tcacggctct gaacggcgcg
     2701 ggcgccctgg ctgtgtgacc gccgacaaac accgcgggcg aaacgcgcaa agaagaagcc
     2761 ggacatttct gtccggcttc cgggaaagtc aatggctgtt tacgctgcgt acccgcgaat
     2821 tagcggcggc gcaggaacag gccggccagg ctcagcagaa tcaagctggc aggctccgga
     2881 gcgaacgtga agtccggcag cgagctggct gagccccagt cgtccccgcg cagaccgatc
     2941 ggctcatcca gaccgaagta gatccggtcg acttcgacgt cgccgccctg gcgagaaacg
     3001 ccaccctcat gcacaccgga ggcttcaccc ataatggcaa agaacacgtt ctttggacca
     3061 tccgaaccat cgtagctaaa ggtcacattg cccagccagt agttgaacgg agtctcagtg
     3121 ccgtagctcc accagtccgt ggtaggcagg ccgagttcac gcgggaacca gccgcccagg
     3181 ccgaagccct gcgagactgc caccaacagg aagtcgttgg gctgaccctt attaggtccg
     3241 aaatcggagc cggtttccca gcgacgctgg acacccaggc cttgctggtg attgaaattg
     3301 tccattagca gatcgctgat gaccacgcca tcatcgccca cgattttgag gccaatgccg
     3361 ttccagacgt ccaacgcttc gccagcctca ttgagggcaa cctgtgccca gatacccacg
     3421 gtgtaggtgc ccggcagagc aggctgatcg tacggcagcg tggaatcagg cacaaaatcg
     3481 gccgtggctg cgaccgtctg cggccggccg aagaaaaggg tgaggtcagc tgcgttcgcg
     3541 accatcgcga acattcctaa gactgcaaac gctacgagaa attttctcat cttccattct
     3601 ccttcaaaag gtttctcgaa atggagtgct tccgcctctc gcgatacgaa acgccgcagc
     3661 gcccgctccc tacgggcgac cgccaagcat ggcgcgtcga acatccgatg ttacgagcaa
     3721 cagcacggcc ccgcaactcg cgggggcgtg agcgcgtaag ccccatcagc cgccaatttc
     3781 gcgagtccgc ctccgaaggt ttggccgggc gccggcccag aacgcctcaa agcgtttgcc
     3841 gtacgaacgc actgtaatcc agaccagaaa caggccggcc gagccgcccg cgatggtact
     3901 tatggcttca ggcgagacct tgcctcgcgc aggcggcgca ggtcttcctc gcactgccgg
     3961 agggtcggat cgatctccag cgcctgcacc aggtaacgct cggcctccgc aaagcgccgc
     4021 tggtgactgc gcagcacacc caacgtgtgc agcagacgcg cattgcgcgg ctcaatgttc
     4081 agcgccaact gcaaatgctg gtccgcctcg gcaaagcggc ccaggcgagt cagcgaaatg
     4141 gccatattgt gcaacatgcg cggatcttgt gcgttgtcgg aaagcactat acggaactgc
     4201 tccgccgctt cctgataacg ccccgcctcg ttcaacgccg aagcgtacgt gccgcgcagc
     4261 ccccggtcat ccggcgccag ggccaacgct cgtttcagta ccggcagcgc ttcctgtggg
     4321 cgccgttgga agatcaaagc catggcctgg agctcgcaca cttgcgtaga gttctgcaac
     4381 gactccacca cggcgcggag catctcttcg gccgcctggt actcgccgcg atttatggcc
     4441 tggcgggcct gctgcgtaag ttcaatgtag gccgcataat ccgcggggtt acccccctgc
     4501 ggttcaaaac gctctatctc tggaccgtcg gattcctcgc gcacttgcgt gccggcgtag
     4561 cccagtgact ccagccgcgc ccgatcggcc gcgctcaggg ccggcgcggg gcctgtgtcg
     4621 cgaaacgccg gcggcgcctg tgcgagcagt tcacgcagtt cctgacgcat gttttcggcg
     4681 acctccggct gctctgtggc cagcgagtgc aactcggctg gatcggcgga caggtcatac
     4741 agctcagggg tcggagcgag gatgtacttc cattcgcggg tcgtcgccgc ccgcaggtgc
     4801 gacaggccga aggcgtcacg cggttcaagc gtctcgctat aagcaaaaag gtcgccggtc
     4861 cgctggccgc tgatccaggg tagcaggctt tcgccttccg cgttttccag cggaggcagg
     4921 ccgagcagcg ccagaatcgt gggcgcgata tcaatgacgc gcaccacgtc tgccaccgac
     4981 gtgccgggcg cgatcttcca gggacaacgg atcatcagcg gcacgtgcag cgtcgtgtga
     5041 tatagaaaat agccgtgctc cgattcgccg tgctcgccca acccctcgcc gtgatcaccg
     5101 gccagcacta ccagggtgcg ctggtccagc cccaggctcc gcagctccgc cagcacccgc
     5161 cccacctgct catccataaa cgcgatctcg tcctcgtagg cctcggccgg cgtgtacggt
     5221 ggcgtgcgcg ttgtttcgta tggcgtgtgc ggatcgtaga agtgcaccca gaggaagaac
     5281 ggatcgcctg ccagcgaacg cagcatctcg atggcgtcgg cggcgacctc gtcgcctttg
     5341 cgctgcgccc gttcggggtc gcccggcgtc aaggcgctaa cgtcgtggta cacgtcaaaa
     5401 ccttgcgcaa tcccgaactg gcggttcagg accatcgtgg caaccgtcgc ctgcgcggca
     5461 tagccagctt gcttgagcac ttcggccaac gtgacgttcg cttccggcag gcgctcacgc
     5521 ccgttgctcc gggcgttgtg cacgtaaggg ctgttgccgg tcatcaggct ggcgtgcgac
     5581 ggcagcgtca gcggggcggg cgccatgcag cgctcgaaca gcacgccttc acctgccagc
     5641 cggtcgatgt ttggcgtgcg gccgttcggc cggccgtagc agccaaggaa atcggcccga
     5701 gtggtgtcga gcgtgatcag cagtacgttc aggcccgttc cgataaccgt tccgccggca
     5761 tttgggtgca gatagcggaa aagtgcggcc gcgacgataa cgaggagggc gcccgccgcc
     5821 agcgcaaagg gccataaccg ccggcgcggc cgcgtgggcg gctgtgccgg cgggattttc
     5881 ttcacgagtg cgggtcgttt gcgattgcgc ggcgtcatgc gcgttcccgt ataatggctc
     5941 gcccgcgctg ggccgcgcgt gcgggctcag tatatattca cgtcccccag tgcggtagat
     6001 atgagcggta gatacggtct ggttccccaa agcggcagca gagccggctc gtgtcgagcc
     6061 ggagtgagcc gaagtactac accgggcgca ggtgataaag tgctgcggtg agtgggaagg
     6121 cgcgcgccgc ggcccccctt ttcactctat cacgctttca ctttatcact gttccgccgc
     6181 gcccgctgcg tcatcgtgcg ccgctccccg aagacgcgcg aaacgcgcgc aaaagagaag
     6241 ccggacattt ctgtccggct tccgggaaag tcaatggctg tttacgctgc gtacccgcga
     6301 attagcggcg gcgcaggaac aggccggcca agctcagcag gatcaggctg gcgggctccg
     6361 gagtgaacgt gaagtccggc agcgagctga tggcgccaaa ctggtagccg aagaggccaa
     6421 ccggctcatc cagaccgaag tagatcaggt cttctgccgg gacgccaccc cggcgagcga
     6481 tgccgccagt accaatctgg aagtacacgt tcttcgcatc cgtgccgtcg taggacagcg
     6541 tcacgttgcc gagccagtac tgctggggac cagaggcgtc agggtcggca caataggccc
     6601 accaatcggt accgctgacg gcgccgccgg tgagttcgcg cgcccacagg ccgcccaggc
     6661 cgaagcgggt cacggccacc aggtagaagt cggtggggct gccgccaggg ccgaagtcgg
     6721 agccgtcttc ccagcgaaag gcggtgccgg caccgggcct gtgatcgaag ttgtcgatcg
     6781 ccagaccgct gacgaccacg ccctcatcgg ccaggatgtt gatgtgcatg ccgttccaag
     6841 cgtcccacgt ctcgccaggc tgatagtaat tgacctgcgc ccagataccc aaggtgtagg
     6901 tgcccgggtc ggccggctga gccaccggga gggcggcatc tgccacgaaa tcggtcgtgg
     6961 ctacgaccgt ctgcggccgg ccaaagtaga aggtgatgtc attcgcgttc gcgaccatcg
     7021 cgaacattcc taagactgca aacgctacga gaaattttct catcttccat tctccctcaa
     7081 aaggtttctc gaaatggagt gcttccgcct ccgttggacc aagggtgggt gccgctcacc
     7141 cgtactctca cgctgcttac tatactccca tcccagcccg catatcaagc ttcatttgaa
     7201 aaaaaagact atctggcgaa attagtcgca atcgtcctat aagcgcttcg aatatcggcc
     7261 ggaattcggg tgcggttgta gtctcataag ttgtcgcacg ccacatccag agatgttctt
     7321 ttcccgcgat ttcgttggcc cgataagaca tttcgcacgg atcttgtggc ctgcgcggtc
     7381 ggccccagtt gcggccggcc aactggttat cggccgcctc tcgaaccgct ctgaggcgca
     7441 ataccaggtg ccgataacca cacatcctcc ccgactgagg tggatgccga ggccgcactt
     7501 gacgcattgc cgccacgcct ggttatagga cgatcactcg ccgaagggcc acttcaccgc
     7561 cctgcccaga cctgtgatac cccggcgact cactggttcc ccggaagggt tcggcacctg
     7621 gtcagcccgc cggcctgact ctctgaatcc cccggcctcg agccctacct ctgggcactg
     7681 ccgggatcgc accgcacgtc aaatacctct acgccatcca cgcccgcctc cgcgaccatc
     7741 catgtcgaag ccctggccct ccgctatgcg aacgcggcgg ccaaccgtgg gccgccgcgt
     7801 cctagaggag cagagtcgct aagtcagagc gtctacggct catcgaccgg agcggcgggc
     7861 ggcgtcgccg gctcacccac ggtcggtacg caatagtcat ccgcaaggtg cttttcttgc
     7921 ggcgagtatt tcaggatcaa cttgctgacc acgtcacgca gcagagtgcg ctggtcggcg
     7981 gcatttatcc gcgggccgcc ttcggccagg gccgcgttga ccttttcccg cgaaaacggc
     8041 ttaatctccg ccctttccgc cagttcgcgc tgcgcattgg ctgtggccgc gtccaccatg
     8101 tgcgggaaat tcggcgcgcc gaaccagatc ggcgtattag gcaagtccac ccgccctggc
     8161 acgatgacta tcacgtgccc ggattcatgc ccgcggttga gccgctcggc cggttgattg
     8221 cccacataga gcaccggcat cgcgatctcg cgcggataca caaagtcctt gttcactttg
     8281 agcaccagca gcgtgccctc ggtcacccac gggcgctcct tgctgaagat atacctgtaa
     8341 ccttgctcca acgtgaacgg ccgcgcgtag acgatgtcct ccaccggggc cggcgtcgtt
     8401 ggaatgtgcg gcggcgcggc cagagcgccg gctaccgcgg ccatcaggca aatactgagt
     8461 attgttgttc tcatctgtag ctatccttct actgccattc ggcggtcttc attctgttcc
     8521 gctacgaacc taatcacact gcacgtcgaa tatctcaact cggtcgacgc ccgcctcggt
     8581 gagcgagtta ttcggcgagt ccactgtcgt gaagcgcacc cgcacggtcg ccgtcagcgg
     8641 gatgaagtcg gcgaaataca cctgccgcag ctcccagtcc gtgtggctgg ccaggtgcgc
     8701 cgcttgaacc cacgttgcac cgtcgtcgct cgagacaaac acgtccagga aatcctgggc
     8761 cggcggcaac gggtcgtcgc aagtgaacca gttggcgaac cgcatcaccg gattgcccgt
     8821 gccgctcaga tccagcagcg gcgaaatcaa atgcgtcggg ccgccgtcca cgtcgtaagt
     8881 cgggcgattg tcggtcacgt agcatcggcc agagccgtcg aaatcatgag gcggcgagtt
     8941 gtcagcccag ccgccgggga tggcgcgctg ccagatgccg ctggtcattc ctgcctggct
     9001 ttgcacactc cagccagagt cggcctcgaa gtcgtcatcc agaattggaa tgtagtttcc
     9061 gacgtacgac acgtacgggt tgcccgaacc gcccggaggc aggtagaacg tgccgcttac
     9121 gtcgccttgg gcggtgaaat aatattccgg cgcatcgccg cattcgggcg ccggcagcgt
     9181 gccgcgccat agttcgccgg caatgcgctc cagcggcgcc gacagccatg ccccgccgtc
     9241 gtagcgatag tgaattgtgg gcgagccggc gaccacgcta tcgtcgcctt caaatatctc
     9301 cacatttatg tgcgtcggaa cgtgcggcgg aacaaactcc ggggccgcac cgggcaaggc
     9361 catgctcatc ggcacgaagt acgccggata gtcatttgtg agcatcagct catccagcac
     9421 aatccgccct tcgttcgagc cccaagccgg gccgaagttg aaacgcaccg ccacaatgtt
     9481 ggccaaatca aggccggtgc cattggtcag aaaatcggtc ggccgaatgc ggataacctc
     9541 gaagtcgtca tgccatccgg cgccgctccc ggcccccgtg cgctgataag gttcctcaaa
     9601 cccgccgccg taggccccga tgttgatcga acttgtggtg ccagccgcgt cgcgcagcgt
     9661 gacggtaaaa gtcaggtcgc ccagcacggc cgtcgtgtag ggatgccggg tacactgcgc
     9721 cccgcggaac gagaggtacg tgtaggcggc gaagttacgc gcggcgggca cgacttccca
     9781 ctcgtagtag acgttttcac cgttccaatc aaacacgacg ccgcggctgg tatcgccgga
     9841 ggaagcgtgg gtagcgccgt tgaacgggtc gccggacatc caggtgaaat cgctgttggc
     9901 atcgtccagg ttgccctcga ccacgttatg caccgtgtag gttacgcttc cgcccgaact
     9961 gctgacgttc aagccgggcc cggtctgata gtcatcgatg aagaagttgc ctttatcagc
    10021 gccgttgcgg tactcgtgac tcaccacaac gcatgggttg gaggtatcca cgccgatcgg
    10081 atgaaagcgc tcgtattgcc gccagaggaa atcagaagcg ggcacattgc cctcggcgaa
    10141 gtacttgatc agcggcaaga acaatccgag ctggatgcgg tgcgtgatcg tcttattcag
    10201 cgcgcacggt ccatcgaaga agctgggttc agccggtccg tcatgcagcc acgcgtgtcc
    10261 aacaccctgc agcaccgtcg acatgcggta cttagtcgcg cggtcgtgct gcaggaacgt
    10321 ctgcaactgc tcgtcgtacg gcgcgccgct gatatccgtg tcgccggccg ccgtccacag
    10381 atggaaattg gcgtgatgcg gggtagcctc ggccggtcca cccatggcgt tcggcaacat
    10441 ggagctgatc agcacaatgc tgtcaggcgt gtagtgtgtg ggcgtgtatt caccgtccat
    10501 gagtgcgtcg aacgccccca ccacgcccat gccgcccaag ctgtggccga tccagatgat
    10561 gcggctcgca tcaatcttcc cgttcagcac accgccggcg atctcatcct gcttgtcgag
    10621 aatcgcatca gtgtgcccca gcgtgcagtc gggcggccca tagcagttca tatgcgacat
    10681 gacaatgtag ccgtatgagg ccatgtgatg cccgatatgg ctataccact ggtagttgtg
    10741 gccgccgccg tgcccgacga cgatcaacgg gcggggcggc atgctggcga tgtcgctcgg
    10801 gtaaaagagg acttcgttcg tgtaggagcc cggaatgccg aagaccggcc cgacgctgta
    10861 agacgggacg tcggtcaccg ccagcggccc aggctgcgtg gtgtcatgca ccacgtacag
    10921 gccggcctcg cgccccaggc cgtcgatgta gtcgccgccg ctgagctgcc cgtcctggtc
    10981 gaaatccacc accatgtcgt acggggcgcc caggccggtg aagtcgccgg tttcagggtc
    11041 gaacaccgcg gcgttcaatt cattcggccc cgagatcacg aatgtattgt cctggatcgt
    11101 ctcgccgcca aaggtgacgg tcagcgggcc gtcggcggtc atatcgatca gttccgggtc
    11161 aaaggcccac aggccggcac tcttgtgctc gacgatgtag acatccgcgg tccggcccac
    11221 aatatcgggg tgctgcgtcg gatcgatcgc cagctcgacc gtggcgttct ggttgaaggc
    11281 cttcacgtac tcgaagtacg ggtacagcgt gagcgaattg ccggccagtt gcaccggaac
    11341 aatctcgccc tgtccgccgc cgacgacaat ctccacgaac gggtcaatgt cgaacgcgtt
    11401 gaccaggccg tcgccattgg cgtcggccag cagcacgtca catagcggga agctcgccgc
    11461 atagccgtcg gggttcgtca gcgccaggac gaacgggtca atgtcaaatg cgttgacggc
    11521 gccgtcacag ttcaggtcgc cgggcgcaaa cgcat
//
LOCUS       DAOSYS010000103        11518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4776_length_11518_cov_5.159819, whole genome shotgun sequence.
ACCESSION   DAOSYS010000103 DAOSYS010000000
VERSION     DAOSYS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_11518_cov_5.159819"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /gene="rimO"
                     /locus_tag="PLU99_07960"
     CDS             complement(<1..1256)
                     /gene="rimO"
                     /locus_tag="PLU99_07960"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOO17029.1"
                     /translation="MVGLISLGCAKNLVDSEKMLGQIAESGAIISADELAADTVLVNT
                     CGFLGAAREEAMAVIAELAERKRRGQLKRIVVAGCLVQRDGEKIREQIPEVDALVGVH
                     NRDDIPEAVWGRAPARAGRSRPNLFLGDYHAQPWSDTGRLRLTPQHYAYVRISEGCDQ
                     KCTFCTIPSIRGPMRCKQPNELLAECHELITDGARELILIGQDTTSYGRDIGYEPGLA
                     GLLRFLDARCEGARWIRLMYVYPTVLSDEVLDAVAECNRVLKYIDIPLQHINDRMLKA
                     MGRRITRAKTEALLQRIRRRIPDVTIRTTFIVGFPGETEAEFDELLEFVRGFGFDAAG
                     AFKFSFEAETPAARMKEQLPEAVKEERYARFMLAQQEVALAAARAKIGQPLYLAVDGL
                     DEAGRGYAARHAGQAPEVDSVTLLQKG"
     gene            complement(1526..3175)
                     /gene="nadB"
                     /locus_tag="PLU99_07965"
     CDS             complement(1526..3175)
                     /gene="nadB"
                     /locus_tag="PLU99_07965"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOO17030.1"
                     /translation="MSITIGSRRYLTNFEPHRLVHLFTDTIVIGSGVAGLLAALEAAE
                     GGEVLVVTKAAASESATAHAQGGIAAAMSSADSAAEHARDTLAVGCGLSDETIVRQVV
                     AEAPQCIQRLREWGACFDSSGGELDFGREGGHSANRIIHAQGDATGQEVVRVLLERVR
                     RHPRIRIFEHCFSIDLLTHEGQVRGTIAFHPKYGHQMYWATTTILATGGVGRLFRETT
                     NPSVATGDGLAMAFRAGAVLRDMEMVQFHPTTLYLAGASRALISEAVRGEGAYLLDRA
                     GQRFMLRYDERAELAPRDVVSRAIAAEMKREEAAWMHLDVRHFPPGHFATRFPTIARL
                     LADFDINPERDLIPIRPSAHYLVGGVLIDANAQTTLPGLLACGEVTNSGVHGANRLAS
                     NSLLEGLVFGRRAGRLAAQRAQRNGRIEHPPAVSHLLPQSPRTPLDIADVLNSLRSVM
                     SRNVGVERSADRLRETIEIIEFWARYIMDKLFDEPAAWETQNMLTIALCIASAASART
                     ESRGVHFRTDFPNTDPAWRRHVDLTRYEDGIQIGTSPVVSS"
     gene            complement(3221..3787)
                     /locus_tag="PLU99_07970"
     CDS             complement(3221..3787)
                     /locus_tag="PLU99_07970"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOO17031.1"
                     /translation="MKKQLRQQIRDRLAAVRPADLQARSAAACRLLCETDEYRQSDVI
                     MIFLSMPLEVDTSFIAIQAWRDGKRILAPRVSWEQRRMLPVEIQSLTTDVEPGPMGIR
                     EPTEGMPVPVLDIDLVVVPGLAFDERGNRLGRGRGFYDRFLQHKDMRAVSCALALEDQ
                     IVPEVPVGEHDKRVDMIVTDARVRRFGR"
     gene            complement(3791..5173)
                     /locus_tag="PLU99_07975"
     CDS             complement(3791..5173)
                     /locus_tag="PLU99_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOO17032.1"
                     /translation="MEPSTDLFAEQRAAAFARVQPLAVRMRPSRLDEFVGQEHFLGPG
                     KLLRRMLEADRLTSAIFYGPPGCGKTTLARVIAAYTQAAYETLHAAEAGVKDVRRVID
                     EARQRLTNEDRRTVLFLDEIHRFNRAQQDVLLRDVEEGVLILIGSTTENPFFSVNSPL
                     ISRSQVFKFEPLTAEQLKVLLRRALADEQRGLGKLKAVCDEDALDLIAERADGDARRA
                     LSALEVAVLSQLRSKDGAQRATAAPPHVTRAVAAESMQQKSIEYDPTGDTHYDVASAL
                     IKSMRGSDPDAALYWLARMLESGEDPRFIARRIAICASEDVGNADPQALVLAAAAVQV
                     AELVGLPECEYALAQAAVYIACAPKSNAVTLAIHAARQDVREKPILPVPNHLRDRSYP
                     GAARLGHGEGYQYPHDFPGGFVQQDYLGAARCYYNPTDRGDEARLAEWLRQRRRSSEA
                     AGPDPRPQGS"
     gene            5327..7309
                     /locus_tag="PLU99_07980"
     CDS             5327..7309
                     /locus_tag="PLU99_07980"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOO17033.1"
                     /translation="MQNSTVTTPVSQQTAGIQRKAAELGKLVVRMTAMAGAGHPSSGL
                     SLAHLVTWLMYRHMRYDPANPWHPTADRLVLSEGHAVPIIYAAYADLGGAVGKSPDEA
                     QPLRPADVDTLRMHTGVLDGHPNPAEGFPFFDAATGSLGQGLSVAAGLALAARLDHSK
                     RRIYALIGDGESREGQIAEALDFIMDHKLRNVCAVFNANGQGQADFVSQQQSPQRLLA
                     KLRAYGWQAVMIDGHDPLAIAAAFKKAERARRPFAIVACTVKGWGVSDLQHGNWHGKP
                     LKPDQLDAAYASLDQMAAADERVEVGPPPAPLPPPANMTPRPDPRSVNWPSFEETMNS
                     GGLGKDLEKGKLATRQAYGATLKVAGDLLPQVVVLDGDVSNSTFSNIFAKAHPERFFE
                     AKIAEQNMVSVAAGLSAAGFIPFANSFAKFLARTYDQVELASISRANIKLVGSHAGIT
                     PASDGPSQMALSDVAYFRSYTAVRGDDRTSPLCWCYQPADAVAAYHCTRLMLEHHGMC
                     YMRTHRPSVPLIYAPDTRFEPGGFQVLASGPDVSLVTAGYMVHVGRQVLEMLAKQNIH
                     ATLVDAYCLPLDGERLIEVLRKGGGRTLVFEDNYGGGLGAAVAEAAARDCRVTVSTLC
                     CQRVPRSARGEAEILDYCGVGPGQVADHVLAMLRRP"
     gene            7481..8518
                     /locus_tag="PLU99_07985"
     CDS             7481..8518
                     /locus_tag="PLU99_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO17034.1"
                     /translation="MNLVTGATGLLGSHIVEQLRRRNRPVRVLVRPGADLSWLETQDV
                     EFVEGDITDLDSVRKACQGVDVVYHSAAKVGDWGPWEDFQRVTIDGTRNVITAAADAK
                     VRRLVHISSISVYGYYTKDAVIDETWPTAFGLYKWAYYSKSKVEAERLALEAHRSGRI
                     QVAVIRPAWIYGPRDRTTIARLHHIVSSGKAKILGRGDNRLNVVYAGNVAEAAIAAAD
                     TPEAGGEVFNCSNDGDITQQQYFDLLARACGAPPVQRHVPYRVAYTAGFVLECLGHLL
                     RSKKPPFITRYAVWLMGRRSFFTTAKARRVLKWRPTVSYEEGVPMTVKWYMEQTSGSR
                     ARAKSDKVIMQ"
     gene            complement(8550..9452)
                     /locus_tag="PLU99_07990"
     CDS             complement(8550..9452)
                     /locus_tag="PLU99_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOO17035.1"
                     /translation="MSGAQAEKYLIEAVQRGDQQAWREVIARYQGRLLSFARRMLAQR
                     GDAEDLVQETFLGLLRSLPNYDPARSLETYLFAILRHKLHDQFRKAEHGRRQSLEQLE
                     VDEAPELWLDRDTPSRRVGAQEDLSAQRQALVECLRQWVERCCGQGRFQDLMVVEMLM
                     VLGLRNKEVAEDLELTETAVAGIKFRVLEQWRAVTQAAAVGRDWQESDLAGDSTLAAI
                     WREEGISCPKRSTLGRFLLGALDEEWMTFIDFHANQAQCPRCLANLDDLREEEQRDAA
                     EREKLCERCFASSIGFLSKMPDDQ"
     gene            complement(9518..>11518)
                     /locus_tag="PLU99_07995"
     CDS             complement(9518..>11518)
                     /locus_tag="PLU99_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HOO17036.1"
                     /translation="SGSYTKAIDIYAMGVILFEMLTGRLPFSGSSMGEILMRHLSDRP
                     DLAGIPEPFAAVIAKALAKDPRDRYQDASEMAGALRQFADVTASIASFDHSVLNSVPR
                     RADANDADQTMTTPHRVPPPPPPPLDAWGPPGRADVLSDKLQKKKDKVSEKFRRKAEK
                     LARKAGKSTERVEFGTPEAEPAVDAAPRKVNRVGQLFVILTLMAGVAVGLSAIRGGPR
                     WEEIAVGLFCCIAAAVAGPLVVYYRVLRRSPVRGGVWDRLAYVALTGVMLIPAFAMAS
                     DYLHVAAHHRGEVSPDAHFMRLLVAPLAMLLFCDWTRRLRTGQLGEVRAKDAAWPAVV
                     GLIAASIAGADSFHLAAAVICATASLLTQAGAALWPVRQGVGGTPQAQPPTEELEQKP
                     WAPLAGGTMPPTWARTAPAAATDITPPAVLEADNVLIPETGVSLGGSQERRTVGMAYL
                     LWALCLFGVAGIHRFYSGRYVSGAIWVLTWGLVGIGSLVDLFLIPEMVRASNRAPAGS
                     GRRPSVPRARPISTEVGRPVRTEYVAASRNDWPTVLLLLGGVGLALGLGRPMQTMEEI
                     PSLAAFWNALNEPGHVALMIICLGGLLLAIGRRPAGAAHACRAILACLFSVAGAAAGM
                     VLLPQISFGPDALPALGLIVSPLLVGLCLLLWPPQRERLIVI"
BASE COUNT         2062 a         3936 c         3561 g         1959 t
ORIGIN      
        1 cccttttgca agagcgtgac tgagtcgact tccggcgcct ggccggcgtg ccgcgcggcg
       61 taaccgcgcc cggcttcgtc caaaccgtct accgcgagat agagcggctg gccgattttg
      121 gcgcgcgctg cggccagggc cacttcctgc tgcgccaaca tgaatcgcgc ataacgctcc
      181 tccttgaccg cctcggggag ctgttctttc atgcgcgcgg ccggcgtttc agcctcgaaa
      241 gagaacttga acgcgccggc ggcgtcgaag ccgaaaccgc gcacgaactc cagcaattcg
      301 tcaaattccg cctccgtttc gcctgggaat ccgacgatga aggtcgtccg gatcgtgaca
      361 tcaggaatgc ggcgacgaat tcgctgcagc agggcttcgg ttttggcacg ggtgatccgc
      421 cggcccatcg ccttgagcat gcggtcatta atgtgctgaa gcgggatatc aatgtacttg
      481 aggacccggt tgcactcggc taccgcgtcc aggacttcgt ctgataacac ggtcgggtac
      541 acgtacatca gccttatcca gcgggcgccc tcacaccgcg cgtcgaggaa ccgcaacagc
      601 cccgccaatc cgggctcgta accgatatca cggccgtagc tcgtggtgtc ctggccgatg
      661 aggatcagct cgcgggcgcc gtccgttatg agctcgtggc actcggcgag cagctcgtta
      721 ggttgcttgc agcgcatggg gccgcgaatc gacgggatgg tgcagaacgt gcatttctgg
      781 tcgcagcctt cgctgatgcg tacgtaggcg taatgctgcg gcgtgaggcg caagcggccg
      841 gtatcagacc agggctgcgc gtgatagtcg ccgaggaaaa ggttcgggcg gcttcggccg
      901 gcacgagccg gggctcgacc ccaaacagct tccgggatgt cgtcacggtt gtgtacgccg
      961 accagcgcgt ccacctccgg aatctgctcg cgaatcttct ctccatctcg ctgcaccagg
     1021 cagccggcga ccacgatgcg tttgagttgg ccgcgccgct tgcgttcagc caattcggcg
     1081 attaccgcca tggcctcctc gcgcgccgcg ccgagaaagc cgcaggtatt gacgagcacc
     1141 gtatccgccg ccagctcatc ggcactgatg atggcgccgg actcggcaat ctggcccagc
     1201 attttttctg aatcaaccag gttcttggcg cagccgagcg agataagtcc gaccacgggt
     1261 ggcttgccgg accgcggcgt gtggcggcgt tgtggggcgc tctgggctcc gcccactacc
     1321 ggcaaaggct tgccgacggg ccgcttttgc ccagccgtat cgggtcgaag gtgttctctc
     1381 gcgaaatctg tcatgccctg ccggttgaaa cccattgtcg gattctacga gccattgttg
     1441 gattctaccg gcccataata ccgcggtaat cgccgtgtgg cgccggccga ctggaatccg
     1501 ggccggagtc cgccagccgc gcgggttaac tcgacacgac cgggctggtg ccgatctgga
     1561 tgccgtcttc gtagcgggtc aggtccacgt gccgtcgcca cgccggatcg gtgttcggaa
     1621 aatccgtgcg gaagtggacg ccgcgggatt cggtccgggc cgaggccgcg ctggcgatgc
     1681 acaacgcgat cgtgagcatg ttctgcgttt cccaggccgc cggctcatcg aatagcttgt
     1741 ccatgatgta gcgggcccag aattcgatga tctcaattgt ttcccgcagg cggtcagccg
     1801 agcgctccac cccgacgttg cgcgacatca cgctgcgcag actgttcagc acatcggcga
     1861 tgtcgagcgg cgtacgggga gattgcggca gcaggtggct tacggccggc gggtgctcga
     1921 tgcgcccgtt gcgctgcgcc cgctgcgcgg ccaggcggcc cgcacggcgc ccgaagacca
     1981 gcccttcgag tagggagttg ctggccagcc gatttgcccc atgcacgccg ctgttggtga
     2041 cctcgccgca ggccagcaga ccgggcaggg tcgtttgcgc attggcgtcg atcagaacgc
     2101 caccgaccag atagtgggcg ctggggcgga tggggatcag atcgcgctcc gggttaatgt
     2161 caaaatcggc caggaggcgg gcgatcgtcg gaaaacgggt cgcgaaatgc ccgggcggaa
     2221 agtgccgcac atccaggtgc atccaggcgg cttcctctcg cttcatttcg gctgcgatcg
     2281 cgcggctgac cacgtcgcgc ggggccagct cggcccgctc atcgtagcgc agcatgaaac
     2341 gctgtccggc acgatccaac aggtacgcac cttcgccgcg gactgcttcg ctgatgaggg
     2401 cgcgcgaggc gccggccagg tagagcgtgg tcggatggaa ttgcaccatc tccatgtcac
     2461 gcagaacggc cccggcgcgg aaggccatcg ccagcccgtc acccgttgcg accgacggat
     2521 tcgtcgtttc acggaagagc cgcccgacgc cgcccgtcgc caggatggtc gtggtggccc
     2581 agtacatttg gtggccgtac ttggggtgaa aagcgatcgt gccgcggacc tgcccctcgt
     2641 gcgtcagcag atcgattgaa aagcaatgct cgaagattcg aatacgcggg tgccgccgga
     2701 cgcgctcgag cagcacgcgt accacttcct gcccggtggc atcgccctgc gcgtggatga
     2761 tgcggttggc cgaatgcccg ccctcgcgcc caaaatccaa ttcgcccccg ctgctatcga
     2821 agcacgcccc ccactcacgg agccgttgga tgcactgtgg cgcctccgcc accacttggc
     2881 ggacaatggt ttcatcgctc aagccgcaac ccacggccag cgtatcccgg gcgtgctcgg
     2941 ccgcgctgtc agccgagctc atcgccgcgg cgatgccgcc ctgggcgtgg gcggtggcgg
     3001 attcgctcgc ggctgccttg gtcaccacca gcacttcgcc gccctcggcc gcctcgagcg
     3061 ccgccaagag cccggcgacg ccggagccga tcacaatcgt gtccgtgaac aggtgcacca
     3121 ggcggtgcgg ctcaaagttg gtcagatagc ggcggctgcc gatggtgatg ctcatggcga
     3181 atattgtata tcaagggaat ccgggcgcgc gaaaccggcg ctaccggccg aagcggcgca
     3241 cccgggcgtc ggtcacgatc atgtcgacac gcttgtcgtg ttcgccgacc ggcacttcgg
     3301 gcacgatctg atcctccagc gccaacgcgc acgacacggc ccgcatgtct ttatgttgta
     3361 gaaaacgatc gtaaaagccg cgcccgcggc cgaggcgatt gccgcgctcg tcgaaggcca
     3421 ggccgggcac aacgaccagg tcgatgtcga gtacgggaac gggcatgcct tcggtcggct
     3481 cgcggatgcc catcggcccc ggctcgacgt cggtggtcag cgactgaatc tcaaccggca
     3541 gcatgcggcg ctgctcccac gagacgcgcg gcgcgaggat gcgcttgccg tcgcgccagg
     3601 cctgaatggc gatgaacgac gtatccacct cgagcggcat ggagaggaaa atcatgatca
     3661 cgtcggattg gcggtattcg tcggtttcgc acaacagccg gcacgccgcg gcgctgcggg
     3721 cttgcagatc ggccgggcga accgcggcca agcgatcgcg aatctgctgg cgtaattgct
     3781 tcttcacgct ctacgagcct tgcggccgcg gatctggccc agcggcctcg gacgaccggc
     3841 gccgctggcg cagccattcc gccagccgcg cttcatctcc tcggtcggtg ggattgtaat
     3901 agcagcgggc cgcgccgagg tagtcctgtt gcacgaaccc gccgggaaaa tcgtgggggt
     3961 actgataacc ttcgccgtgc cccagacgcg ccgcgccggg atagctgcgg tcgcgcaggt
     4021 gattcggtac gggaagaatc ggtttttcac gcacgtcctg gcgggcggca tgaatagcga
     4081 gcgtgacggc gttcgatttc ggagcgcatg caatgtagac cgccgcttgg gccagggcgt
     4141 actcgcactc gggcagcccc accagttcgg ccacctggac ggccgccgcc gcgagcacga
     4201 gagcctgcgg atcggcattg ccgacgtctt ctgaagcgca gatcgcgatg cgccgggcga
     4261 tgaagcgcgg gtcttcgccg ctttcgagca tgcgggccag ccagtacagc gcggcgtccg
     4321 ggtcgctgcc gcgcatgctc tttatcaggg ccgaggccac gtcgtaatgc gtgtcgccgg
     4381 tcggatcgta ctcgatggac ttctgctgca tggactccgc ggctacggca cgcgtgacat
     4441 gcgggggcgc ggcggttgca cgctgggcgc cgtccttgga acgtagctgc gaaagcaccg
     4501 cgacttcaag cgcgctaagg gcgcggcggg cgtcgccgtc agcccgctcg gcaatcaggt
     4561 cgagcgcgtc ttcatcgcaa acggctttca gcttgcccag cccgcgctgc tcgtccgcca
     4621 gggcccggcg cagcaagact ttgagttgtt cggctgtcag cggctcgaac ttgaaaacct
     4681 ggctccgcga gatgagcggg ctgttgaccg agaagaacgg gttttcggtg gttgagccga
     4741 tgaggatcaa cacgccctct tcaacatctc tcaggagtac gtcctgctga gcacgattga
     4801 agcggtggat ctcgtcgaga aacagcacgg tgcgccggtc ttcgtttgtc aggcgttggc
     4861 gggcctcatc aatgacgcgc cgcacatcct tcacgcccgc ttccgccgcg tgcagcgttt
     4921 cgtacgcggc ctgcgtgtac gccgcgatca cgcgggcgag cgtggttttg ccgcagccgg
     4981 gtggaccgta gaagatggcg ctggtgagcc ggtcagcctc aagcatgcgc cgcagcagct
     5041 ttcccggccc caagaagtgc tcctgcccca cgaattcatc gagcctgctc gggcgcatgc
     5101 gcacggcgag cggctgcacg cgcgcgaaag ccgctgcccg ctgctcggcg aacaggtcgg
     5161 tcgaaggttc catgcgccga ttatacgacc aaccggccgc gcgccccgcc actttgtgcc
     5221 ttttcacatt gcactggcat caggcgcccg ggtcgagcct acaggttgcc gctttcggcg
     5281 ccccccgata gagtaactgg tctttacccc gcagagcgag tcaaccatgc agaactcgac
     5341 cgtcacaaca cccgtttcgc agcaaacagc cggaattcag cggaaagccg ccgaactcgg
     5401 caagctcgtc gtccgcatga cagccatggc cggcgccggc cacccctcca gcggcctgtc
     5461 gctggcccac ctggtgacct ggctgatgta ccgccacatg cgctacgacc cggccaaccc
     5521 ctggcacccg accgctgatc ggctggtgct gtccgagggc cacgctgtcc cgatcatcta
     5581 cgcagcctac gcggacctcg gcggcgctgt cggtaaatcg cccgacgaag cgcagccgtt
     5641 gcggccggcc gacgtcgaca ccttgcgaat gcacaccggc gttctcgatg gccatcccaa
     5701 tcccgccgaa ggcttcccgt tcttcgacgc cgccaccggc agcctggggc agggccttag
     5761 cgtggccgcc ggcctggcgc tggccgctcg gctggaccac agcaaacgcc gcatctacgc
     5821 cctcatcggc gacggcgaat cgcgcgaagg ccaaattgcc gaagcgctgg acttcatcat
     5881 ggaccacaag ctgcgcaacg tctgcgcagt cttcaacgcc aacggccagg gccaggcgga
     5941 cttcgtctcg caacaacaat cgccgcagcg cttgctcgcg aaattgcggg cttacggctg
     6001 gcaagcggtc atgatcgacg gccacgaccc actggccatc gctgccgctt tcaagaaagc
     6061 cgagcgcgcc cgccgcccgt tcgcaatcgt cgcgtgcacc gtcaagggtt ggggcgtcag
     6121 cgatttgcag cacggcaact ggcacggcaa gccgcttaag ccggatcagc tcgacgccgc
     6181 atacgccagc ctcgaccaaa tggccgccgc cgatgagcgc gtcgaagtcg gcccaccgcc
     6241 ggccccgttg ccgccgcccg ccaacatgac gccgcggcct gacccgcgca gcgtgaactg
     6301 gccctcattt gaggaaacca tgaactcggg cggcctcgga aaagacctgg aaaagggcaa
     6361 acttgccacg cgccaagcct acggcgcgac gttgaaggtc gcgggcgacc tgctaccgca
     6421 ggtggtcgtc ctcgacggcg acgtcagcaa ctcgaccttc tccaacatct ttgccaaagc
     6481 ccacccggag cgcttcttcg aggcgaaaat cgccgaacag aacatggtct ccgtcgccgc
     6541 cggcctgtcc gccgccggat tcatcccctt tgcaaactcg tttgcgaagt tcctggcccg
     6601 cacgtacgac caggtcgaac tggccagcat ctcgcgcgcc aacatcaagc tggtcggctc
     6661 gcacgccggc atcacccccg cctccgacgg ccccagtcag atggccctgt cggacgtcgc
     6721 ctatttccgt tcgtacacgg ccgtgcgcgg cgacgatcgc accagcccgc tgtgctggtg
     6781 ctatcagccc gccgacgcgg tggccgcata ccactgcacg cgcctgatgc tcgaacatca
     6841 cggtatgtgc tacatgcgca cgcaccgccc gagcgtgccg ctgatctacg cgccggatac
     6901 gcgcttcgaa ccgggcggat ttcaggtgct tgcctccggc ccggacgtgt cgctggtgac
     6961 ggccggctac atggtgcacg tcggccggca ggtgctcgag atgctcgcca aacagaacat
     7021 ccacgcgact ttggtcgatg cgtactgcct gccgctggac ggcgagaggc tgattgaggt
     7081 gttgcgcaag ggcggcggcc gaacgctcgt gtttgaagac aactacggcg gaggactcgg
     7141 cgcggccgtg gccgaagctg ccgcccgcga ctgccgcgtg accgtgagca cgctatgctg
     7201 tcagcgcgtg ccgcgctccg cccgcggcga agccgaaata ctggactatt gcggggtagg
     7261 cccgggccag gtagccgacc acgtgctggc gatgctgcgc cgcccgtaga acggtgataa
     7321 agtgatcaag tgatagggtg ataaagggtg gcgcaatggt gataaatgtg gcgctgcggt
     7381 agctgaccgg cctacgcgcg cctttatcac tctatcactt tatcacctta gcaccctctt
     7441 tatcactcta gggagtagac ttgctgaacg aaaggtacac atgaacctcg tcaccggcgc
     7501 aaccggcttg ttgggcagcc acattgttga gcaactgcgg cgcagaaatc gccccgtgcg
     7561 cgtcctggtg cgcccaggcg ccgatctctc ctggctcgag acgcaggacg ttgaattcgt
     7621 cgagggcgat ataacagacc tcgactcagt gcgcaaggcc tgccagggcg ttgacgtcgt
     7681 ttaccattca gccgccaaag taggcgactg gggcccctgg gaagacttcc agcgcgtcac
     7741 catagacggc acgcgcaacg taattacggc cgcggccgat gccaaagtgc gccgcctcgt
     7801 acacatcagc tcaatcagcg tctatggcta ctacaccaaa gacgcggtca tcgacgaaac
     7861 ctggccgacc gccttcggcc tgtacaaatg ggcttactac agcaagtcaa aagtcgaggc
     7921 cgagcgcctc gcactggaag cccaccgcag cggacgcatt caggtggccg tcattcgccc
     7981 tgcctggatt tacggcccgc gcgaccgcac taccatcgcc cggctgcacc acattgtgtc
     8041 cagcggcaag gccaagattc taggccgcgg cgataatcgc ctgaatgtcg tctatgccgg
     8101 taatgtcgcc gaagcggcga tcgcggcggc cgacacaccc gaggccggcg gcgaagtctt
     8161 caactgcagc aacgacggcg acataaccca gcagcagtat ttcgacctgt tggcgcgggc
     8221 ctgcggcgcg ccgcccgtcc aacggcatgt accgtatcgc gtggcgtaca cggccggttt
     8281 tgtgctcgaa tgcctcggcc acctgctgcg cagcaagaag ccgccgttca tcacgcgtta
     8341 cgcggtctgg ctcatgggcc gccgctcgtt cttcacgacc gccaaagccc gccgcgttct
     8401 gaaatggcgc ccgacggtga gctatgaaga gggcgtgccg atgacggtga agtggtacat
     8461 ggagcagacc agcggcagcc gtgcacgggc caagagtgat aaggtgataa tgcaatagac
     8521 tgataagggc gccccagtct tgagggcgct cattggtcgt cgggcatttt tgaaaggaag
     8581 ccaatgctcg atgcaaagca gcgctcgcac agcttctcgc gctccgcggc gtcgcgctgt
     8641 tcttcctcgc gcaggtcgtc caggttagcc aggcagcgcg gacactgagc ctggttggcg
     8701 tggaagtcga taaacgtcat ccactcctca tccagcgcgc ccaacaagaa ccgccccagc
     8761 gtcgaacgct tcgggcacga aatcccttcc tcgcgccaga tcgccgctaa cgtgctatcc
     8821 ccagccaaat ccgactcctg ccagtcacgc ccaaccgcgg ccgcctgcgt caccgcccgc
     8881 cactgctcca gcacgcggaa cttgatcccc gccacggccg tctcggtcag ctccaggtcc
     8941 tcggccacct ccttgtttcg caagccgagc accatcagca tttcgaccac catcaaatcc
     9001 tgaaaacgcc cttgcccgca gcagcgctcc acccattgcc gcagacactc gaccagcgcc
     9061 tgccgctgcg cgctcaggtc ctcttgcgcg ccgacccgcc ggctgggcgt gtcacggtcc
     9121 agccacagct cgggggcttc atcaacctct agctgttcca gactctgccg ccggccgtgc
     9181 tctgctttgc gaaactggtc gtgcagcttg tgccgcaaaa tggcaaacag atacgtctcg
     9241 agcgagcggg ccggatcata attcggcagg ctgcgcagca gccccaggaa cgtctcctgc
     9301 accaggtctt ccgcatcgcc gcgctgcgcc agcatccgcc gtgcaaagct cagcaaccgg
     9361 ccctgataac gcgcgatgac ttcgcgccag gcctgctggt ccccgcgctg caccgcctcg
     9421 atcaaatact tttccgcctg cgcgcccgac atgccgggca ggataaccgc cggagctaca
     9481 cctggccaag acgtcccgaa gcaacttcag gccggcttta gatcacgatc aaccgctcgc
     9541 gctgcggcgg ccatagcagc aggcatagcc ccaccaacaa cgggctgaca atcagcccca
     9601 gagccggcag cgcatccggg ccgaaactta tctggggaag cagaaccata cccgcggccg
     9661 caccggccac gctgaagagg cacgccagaa tagcccggca ggcgtgggcg gcgcccgcgg
     9721 ggcggcggcc aatcgccaac agcagaccgc ccagacaaat gatcatcagc gcaacgtgcc
     9781 cgggctcgtt gagcgcattc cagaaagccg ctaggctcgg tatttcctcc atggtttgca
     9841 tgggccgtcc cagccccagc gccagcccga cgccgcccag caacagcagc acggtgggcc
     9901 agtcattgcg actggctgcc acgtactcag ttctaaccgg gcgcccaact tcagtcgaga
     9961 tcggacgcgc gcggggaact gacgggcgcc gaccactacc ggccggcgcg cgattgctcg
    10021 cccgcaccat ctcgggaatc aggaagaggt ccacgagcga gccgatcccc actaatcccc
    10081 aagtcaatac ccagatcgcg cctgagacgt accgcccgct gtaaaagcgg tgtatgcccg
    10141 ccactccaaa caagcacagg gcccacaaca aatacgccat tcccactgtg cgccgttctt
    10201 gcgagccgcc taatgagacg cctgtctccg ggatcaagac gttatccgct tcgaggacgg
    10261 ccggcggcgt aatatccgtt gctgcggccg gcgcagtgcg agcccacgtt ggcggcatcg
    10321 ttccgcccgc cagtggcgcc cacggtttct gttccaattc ttcagtcggg ggctgtgctt
    10381 gcggggtgcc gccaacacct tggcgaacgg gccagagtgc cgcgcccgcc tgcgttagca
    10441 gcgacgccgt cgcgcaaatg accgccgccg ccaggtggaa gctatccgcg cccgcgatgc
    10501 ttgcggcgat tagccccacc acggccggcc aagccgcatc cttggcccgg acctcaccca
    10561 actgcccggt gcgcagccgg cgcgtccagt cgcagaaaag cagcatcgcc aaaggcgcca
    10621 cgagcaaacg catgaaatgg gcgtcgggcg agacttcccc acggtgatgg gccgcaacgt
    10681 gcaggtagtc gctcgccatt gcaaacgccg gaatcagcat cacccccgtc aatgctacat
    10741 atgccagccg gtcccatacg ccgccgcgca ccggcgagcg ccgcagtacg cggtaataga
    10801 ccaccaacgg accggcgact gcggccgcaa tgcaacagaa cagcccgacc gcgatttctt
    10861 cccagcgcgg cccgcctcgg atggcgctta gacccaccgc cacgcccgcc atcagcgtca
    10921 aaatcacgaa caactgccct actcgattga ccttccgggg cgccgcgtcc acggccggct
    10981 cggcttccgg cgttccgaac tctacccgct cagtggattt gcccgcctta cgcgccagct
    11041 tctccgcctt gcgccggaac ttctccgaga ccttgtcctt cttcttttgc agcttgtccg
    11101 acagcacatc cgcacgcccc ggtggccccc aggcgtccag cggtggcggc ggtggcggcg
    11161 gaacccggtg cggcgtcgtc attgtctggt ccgcatcgtt cgcatccgcc cgtcgcggca
    11221 cactgttcag cacgctgtga tcgaacgagg caatactcgc ggtcacgtcc gcgaactgcc
    11281 gcagcgcacc ggccatttca ctcgcgtcct ggtaccggtc gcgcgggtct ttggccaacg
    11341 ctttcgcgat gaccgccgcg aacggctcgg gaattcccgc aagatccggc cgatcgctga
    11401 gatgccgcat gagaatctcg cccatgctcg aaccgctgaa tggcagccgg ccggtgagca
    11461 tctcaaacaa aatcacgccc atggcgtaga tgtcgatagc cttggtgtag ctgcccga
//
LOCUS       DAOSYS010000104        11507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4782_length_11507_cov_5.258557, whole genome shotgun sequence.
ACCESSION   DAOSYS010000104 DAOSYS010000000
VERSION     DAOSYS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_11507_cov_5.258557"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLU99_08000"
     CDS             <1..403
                     /locus_tag="PLU99_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOO17037.1"
                     /translation="NLTYWRNEPYLGVGPSAAGYLAGERYKNVADVAEYVRRIAAGAD
                     PREETERLPPEKCAGETVMLALRTVEGLSRARFAERFGQDPLTWFAQRNGGSAIAQNV
                     ANGLLEVTPEAIRLTRRGMLVANDVMAEFLE"
     gene            complement(599..1180)
                     /locus_tag="PLU99_08005"
     CDS             complement(599..1180)
                     /locus_tag="PLU99_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HOO17038.1"
                     /translation="MADGEKFLTIVSGLPRSGTSMMMRMLEAGGIPALIDNIRKPDED
                     NPRGYYEYEPVKQTRKDPSWLKQAGGRVVKMVYRLLYDLPPDYQYRVVFMRRKLEEVI
                     ASQNVMLARQAKPGGSLSDDKLMAVFRTELESVYRWLEKQPNFRVLFVDYNEIMADAG
                     PAVDALNRFLGGNLNTAAMRNVVEPELYRQRRA"
     gene            1787..2929
                     /locus_tag="PLU99_08010"
     CDS             1787..2929
                     /locus_tag="PLU99_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HOO17039.1"
                     /translation="MEPHASSPAAEKPPAVKEAVKELHILWTPDGMSCDGDTISVTAA
                     TLPSIEDVVLGAIPGLPKVHLHNRVLDAQQGGDQIMHYWHQAAEGTLGAPFILVVEGS
                     IPNEEIKSQGYWTAMGVDKRTGQPIRLNDWIDRLAPKAWAVVAAGTCATYGGVHAMQG
                     NPTGSMGLADYLGWDFRSQAGLPIVNVPGCPVQPDNFMETLLYLLYQAAGLAPMIPLD
                     ELLRPTWLFGKTVHEGCDRAGYYEQGDVAREYGSPKCIVKIGCWGPVINCNVTKRGWM
                     DGIGGCPNVGGICIACTMPGFPDKFMPFMDEPPGAKMSSAVVLTYGATIRRLREITNR
                     TASREPRWRRRTTVLNTGYHPTTYHEPAAATVEKTMVETPITGGAS"
     gene            2926..3720
                     /locus_tag="PLU99_08015"
     CDS             2926..3720
                     /locus_tag="PLU99_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOO17040.1"
                     /translation="MTSIAQPETIGHSGQRKLIDVNWDPVTRIVGNLGIYTKIDFERK
                     EVAECYTTSSMFRGYSVFMKGKDPRDAHFISSRICGICGDSHATCAVYAQNMAFGIST
                     PRMGEWILNLGEAAEYMFDHCLFQENLVGADFCEQAMADVYAGRTQTWADFKVPDEAL
                     GCGFHEATRGVLSHHLVIRDGLIANYHPYPPTPWNASPRDVYGTPGPYEDAVQNTPIF
                     EENGPDDFKGVDIMRAVRSFDPCLPCGVHMFLGHGKLLKTLSGICY"
     gene            3767..4615
                     /locus_tag="PLU99_08020"
     CDS             3767..4615
                     /locus_tag="PLU99_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOO17041.1"
                     /translation="MAGLNMATPINPSDLRGKRGDTPKKPYRQILIDEMTEGLHAMQR
                     SVTGLSLSGLSAGLDIGFSLFLMAAMQTLAGEYFSQPVLRMVNANLYAVGFIFVVLGR
                     SELFTEQTTLAVLPVLGRHASLARLGRLWAIVYVSNLVGAALFAELTVLIGPRLQIID
                     VAALGEIAAAVTEHPWLAILLSAVLAGWLMGLMSWLVAASRDTISQIVVVWLVATVIG
                     FTQLHHAIVGSVEVLAGVFAGRATLASFGHFLLWATIGNAIGGGVFVAVLKYSHATRE
                     EPADVG"
     gene            4605..5243
                     /locus_tag="PLU99_08025"
     CDS             4605..5243
                     /locus_tag="PLU99_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOO17042.1"
                     /translation="MWGKEQFMNMAEPDAKLMQAMLRIDALLQRVEQLPDPAVRANVT
                     DILQSLLDLHGQALARMLELFGRRGDQAQPIFDALEADQLISGVLLVHGLHPRSIEQR
                     VQRALQQIQPYLAAHNGGVELLEVTGEQVVRLRLAGDCRACPGSRATLDNLIKEAIYL
                     AAPDVAAIEVEGAAPDTDAAAASAPPKGQAERECPRVASPFSHSSFNAEQRS"
     gene            5536..6273
                     /locus_tag="PLU99_08030"
     CDS             5536..6273
                     /locus_tag="PLU99_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HOO17043.1"
                     /translation="MPDLDFAVEGASVEPYAAAPLLLLKVRATDRDPQPLPIHSVLLY
                     SQARIEPAQRRYCPAEQAHLRELFGFPEHWPRSLRSLLWTHTQSTVPGFTGGTLFDLP
                     VPCSSDLSLAVTKYCNALENGELPLLLLFSGTVFYAAPHAPLQAAPIGRDREAPFRLP
                     VRVWKDLMDRYYPNSAWLCLRRDLIERLQHYKLRAGLLSWDETLERLLDAEHRAHPAP
                     PAVPEPLAGAPVTAEVQSKAVKAGAPA"
     gene            6270..6632
                     /locus_tag="PLU99_08035"
     CDS             6270..6632
                     /locus_tag="PLU99_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17044.1"
                     /translation="MSDCQSGPTNGGRPVPINSRHPATDDSKLQSAVVNGIEVAAGDR
                     VRLRPQQRADAVDLLLVGRTATVVSIEHDFEGRQYLAVTVDDDPGRDLGPAGWPGHRF
                     FFRPDELEPLPSQPGGVP"
     gene            6629..7285
                     /locus_tag="PLU99_08040"
     CDS             6629..7285
                     /locus_tag="PLU99_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOO17045.1"
                     /translation="MTPRILVAGIGNIFLGDDAFGVEVIQRLAQRTWPEGVRIVDFGI
                     RGFDLAAALLEGWDYAILIDTAPRGAAPGTLYVIEPELDREGEAPAEPRSEAGNSAES
                     RRGGAFALPAIAPAGALDGHRLDPVGVLALVKLLGGPLPRILLVGCEPARGSGTDEVT
                     AELSAPVRAAVVESIKLVESLIAALRRPDPRSQSAFTALFQGRTEHGCHPHEAHAAPS
                     "
     gene            7245..7418
                     /locus_tag="PLU99_08045"
     CDS             7245..7418
                     /locus_tag="PLU99_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17046.1"
                     /translation="MVAIRTKPMRRRVRETTDVVTAHPGLTVAAIILLSIGGAIFVAM
                     YPEMKRYLLAKRM"
     gene            7747..8958
                     /locus_tag="PLU99_08050"
     CDS             7747..8958
                     /locus_tag="PLU99_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO17047.1"
                     /translation="MYSSLSDDSAKTPTVTRRQFVQIGAVGAGVLLGTPHLWAQQASQ
                     PSPPPKPKTNIDDALKVARTKYSLPGLFPGKVVHVQDAQAVTDGRIVPSAVERMFARG
                     LQELTGQNSSESFNLLFTKDDVVGIKVNPAGAGIISTRPEVVDVVIAWLLGNGLPKQN
                     IIIWDRFDMMLKDAGYTPERFPGIKIEGLQTMVEKLPEGDNADHSAWLDKDGNHISAG
                     NFDQDVYYWADVEGPKDLPYLNQHVFNGKYSYFGKLVTQKLTKIINIPVFKNTGNGVS
                     MATKNLGYGAICNTNRLHTPLFFDVCTEVLAFPALRDKLVLNIIDGLRGQYEGGPMPA
                     AQFVYDYNSLFFATDPFAADSVGHELMVQKRKEMGIKVNEHPRFTEYLRYAERLGLGV
                     VDPAKVQVRHV"
     gene            8963..9919
                     /locus_tag="PLU99_08055"
     CDS             8963..9919
                     /locus_tag="PLU99_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17048.1"
                     /translation="MPKAARTFGLALALLPVLALAACRPASAPPMITAAADSTPPATS
                     SAPTPQRESSELQWTEVERRQFVGQDLFNHIDGAAEVFLEMGFREVLVRLYSNGQAKL
                     VHEVYEMKDPTAARGIYLRFRGRGTPVAGVSGRNFGNRYQIGAQKDRYFVQITDASGR
                     GESLSAMTRLVNQALAAIPHDGDVPLLDLLPAEGLVPNSEVIVCGPYSLQSVFTLGEG
                     DILQLQGQRCGIAGDYVTEHEGSVTRLIVPYGSAAAAGTAFQHLLHGLDSELQVLHRN
                     EHTAVFREKNGQFGSITARDDKLDIRLHLVRDPGLASQPTVP"
     gene            complement(9938..11254)
                     /locus_tag="PLU99_08060"
     CDS             complement(9938..11254)
                     /locus_tag="PLU99_08060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17049.1"
                     /translation="MTVAPGLNSAHSKAGLWLFILALLLRAGWVGFQWHRTGPAFEFD
                     DERLHWAMASNLVDAGQMVTDDGRFAARMPLYPLFLAIFAGAGQTGVLFARLAQAALG
                     AATVWLIYRLARDALGPRPALLAGGLACIDPYGIFFTNLLLSEVLFTLIAVALTAAAW
                     RLIRPVNGDGRRDDAAPVDDHSFVDDPSSSPTFRKSALAVAVLGPAAILTRPSALGWI
                     ISLWLLLWLYDRNKRRAALCVVAYAAALGVFLLPWGLRNHHAIGSYAWLSTNGGVTLY
                     DAQGPQADGSSNQQFLEKMPGLSGLGEAERDRLLRDKALEQMKRDPARVVHLAGVKFL
                     RTWNPVPNFEAYRGGLTAIASALFTTLVLLLAAVAIWRTAFVRRSRTGVRYQTLVWLP
                     IVYFTLLHCIFIGSLRYRVPLMPFLELSAAAAVCAICPKPKRTASA"
     gene            complement(11297..>11507)
                     /gene="tilS"
                     /locus_tag="PLU99_08065"
     CDS             complement(11297..>11507)
                     /gene="tilS"
                     /locus_tag="PLU99_08065"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOO17050.1"
                     /translation="GAPGAKSVSDFLIDEKVEPAARSRTGLLCDQDGPVWIMPLRIDE
                     RVKLTPATQRALRVTLRPAKNRQVT"
BASE COUNT         2201 a         3726 c         3580 g         2000 t
ORIGIN      
        1 caatctgacc tattggcgaa acgaacccta ccttggcgtg ggcccgtccg ccgccggcta
       61 cctggctggc gagcgctaca agaacgtcgc tgacgtggcg gagtacgtgc ggcgcatcgc
      121 ggccggggct gacccgcgcg aagaaacgga gcgtctgccc ccggaaaagt gcgccggcga
      181 gacggtaatg ttagcgctgc gaacggtgga agggctcagc cgggccaggt tcgcagagcg
      241 cttcggccag gacccgctaa cttggtttgc ccagcgaaac ggcggctccg ccatagctca
      301 gaacgtcgcc aacggtctgc tggaggtcac gcccgaagcc atccgcctca cgcgccgcgg
      361 gatgttggtg gcgaacgatg tgatggcgga attcctagag tgataaagtg ataaggtgat
      421 agagtggtaa agcgataccg ggaggaggcg tgaaggcgaa ggtgggaacg cgggaacgcg
      481 ggaaggtgaa aacgtgggaa ggtgacgagg ctagcgcgcc cttatcaccc tatcacgtta
      541 tcaccttatc actatgcacc tcttcgcctt ctcaccctct tatgtcactt cgcttcgttc
      601 acgcccgccg ctgccggtag agctccggct cgaccacgtt tcgcattgcc gcggtgttca
      661 ggttgccgcc gaggaagcgg ttcagcgcgt cgaccgccgg ccccgcatcc gccatgatct
      721 cgttgtagtc aacgaacagc acgcggaagt tcggctgctt ctcgagccag cggtatacgc
      781 tttccaactc ggtcctgaac acggccatca atttgtcgtc gctgagcgag ccgccgggct
      841 tggcctgccg ggccagcatg acgttctgcg acgcgatcac ttcttccagc ttgcgccgca
      901 tgaacacgac gcggtattga tagtccggcg gcaggtcgta cagaaggcgg tagaccattt
      961 tgaccacgcg gccgcccgcc tgcttcagcc acgaggggtc cttgcgggtc tgcttgacgg
     1021 gctcgtactc gtaatagccg cgcggattgt cctcgtcggg tttgcgtatg ttgtcgatga
     1081 gcgcggggat gccgcccgcc tccagcatgc gcatcatcat ggaggttccc gagcgcggca
     1141 gtccggaaac gatcgtcaaa aacttctcac catcggccat gaaccaacct caatcccggc
     1201 ggcttgactc cggcagcccg ccggccgctg cccgactaag ggtcaggcta gcgagctttg
     1261 cgggagcgtg caaccacggg gcgcatagcc cgttcgccag cgcatggcgc tttcgcctca
     1321 ggccgtgcga tcgcgattcc acgccggcgc tgcgccgagc gtcacccact ggtcgctata
     1381 gagcaggcag cgcagcgcgg ccacgcattc ggcgcgtttc ggccgagagc tgtcgctcgc
     1441 tttgcagcga cgacccggcc aatttcgaac cgggctaccg gaatgtcaaa aacatgtaac
     1501 catacgcatc ccgccttcta cgccgctaac atcctctgaa cacacccgca aggcgaaatt
     1561 caactttgtc gtaccccgca cttaatcgtt ctgcggttgc ggtccgccga gctgcgcaag
     1621 cgcggcgatc gccgtgttcc tgcaacatcg aagcgtgccg cctggtaacg acctccaact
     1681 gcgtagccga agtcctggtg ctcggcggca tgctcggcac gctctcgccg tacccatatc
     1741 gccctgccag accaaccgct gcgacgggcc taggaggatc tgcaagatgg aaccacatgc
     1801 atcttcgcca gcagccgaaa agccgccggc ggtgaaagag gcggtcaaag agctccacat
     1861 tctctggact ccggacggca tgagttgcga cggcgacacg atctcagtca ccgccgccac
     1921 gctgccaagc atcgaggacg tggtcctggg agcgattccc gggctgccaa aagtgcatct
     1981 gcacaatcgc gtgctggacg cacagcaagg cggcgaccag atcatgcact actggcacca
     2041 ggccgccgag ggcacgctcg gcgccccgtt catactcgtc gtcgagggct ccattcctaa
     2101 cgaagagatc aagtcccagg gctactggac ggctatgggc gtcgataaac gcaccggaca
     2161 gcccatccgg ctcaacgatt ggatcgaccg cctggcgccc aaagcctggg ccgtagtcgc
     2221 ggccggcact tgcgcgacct acggcggcgt tcacgccatg cagggtaatc cgaccggctc
     2281 gatgggcttg gccgactatc tcggctggga tttccgctcg caagcgggtt tgccgatcgt
     2341 gaatgtcccc ggctgcccgg ttcagccgga caacttcatg gaaaccttgc tgtacctcct
     2401 gtaccaggcg gccggcctgg ccccgatgat cccgctggac gagttgctcc gccccacgtg
     2461 gctcttcggg aaaacggtgc acgaaggctg cgaccgggcc gggtattacg agcagggcga
     2521 cgtcgcccgc gaatacggct cgcccaagtg catcgtgaaa atcggctgct gggggccggt
     2581 catcaactgc aacgtcacca agcgcggctg gatggacggc atcggcggct gcccgaacgt
     2641 gggcggcatc tgcatcgcct gtactatgcc gggctttccg gacaaattca tgcctttcat
     2701 ggatgaaccg cccggggcca agatgtcgag cgcggtcgta ctgacttacg gcgcgaccat
     2761 tcgccgcctg cgcgagatca ccaaccgcac cgccagccgc gaaccgcgct ggcgccgtcg
     2821 caccacagtc ctgaacaccg gctatcaccc gacgacttat cacgaacccg ccgccgccac
     2881 agtcgagaaa accatggtcg aaacgcccat caccggaggt gcgtcatgac aagcatcgcc
     2941 caaccggaaa ccatcggcca cagtggtcag cgcaagctca tcgacgtgaa ctgggacccg
     3001 gtcacacgca tcgtcggcaa cctcggcatt tacaccaaga tcgacttcga gcgcaaggag
     3061 gtcgccgaat gctacaccac ctcgtcgatg tttcgcggct atagcgtctt catgaagggc
     3121 aaggacccgc gcgacgccca tttcataagc agccgcatat gcggcatctg cggcgactca
     3181 cacgccacct gcgcagtgta cgcgcagaac atggccttcg ggatcagcac gccgcgcatg
     3241 ggcgagtgga ttctgaacct gggcgaggcg gccgagtaca tgttcgatca ctgcctgttc
     3301 caggagaacc tggtcggggc cgacttctgc gaacaagcca tggccgacgt ctatgccggc
     3361 cgcacccaga catgggcgga tttcaaggtt ccggacgaag ccctcggctg cggtttccac
     3421 gaggcgacgc gcggcgtctt gtcgcaccac ctggtgatcc gcgacggcct gatcgcgaac
     3481 taccacccct atcccccgac gccctggaac gccagtccgc gcgacgtcta cggcacgccg
     3541 ggcccgtacg aggacgcggt gcagaacacg cccattttcg aggagaacgg cccggacgac
     3601 tttaaaggcg tagacatcat gcgcgcggtc cgcagcttcg acccgtgtct cccctgcggc
     3661 gtccacatgt tcctcggcca cggcaaactg ctgaaaacac tgtcgggcat ttgttactga
     3721 tacggagtca ggagtctttt ccgcgggatt tgtctgatcg atgccaatgg ccgggctcaa
     3781 catggcaaca cccatcaatc cgagcgatct ccgcggtaag cgcggcgaca cgcccaagaa
     3841 gccctaccgg caaatcctga tcgacgagat gaccgaaggt ctgcacgcaa tgcagcgctc
     3901 ggtcaccggc ctgtcgctat ccggactctc agccggactg gacatcggct tcagcctgtt
     3961 cctgatggcg gccatgcaga cgttggccgg cgagtacttc tcgcaacccg tgctgcgcat
     4021 ggtcaacgcc aacctgtatg cggtaggctt cattttcgtc gttctggggc gttcggagtt
     4081 gttcaccgag cagaccacgt tggcagtctt gcccgtgttg ggccggcatg cgtcgctggc
     4141 acggctgggg cgactgtggg ccatcgtata cgtgtcgaac ctggttgggg cggccctgtt
     4201 tgccgaatta accgtgctga tcgggccgcg cttgcagatc attgatgttg cggcgctggg
     4261 cgaaatcgcg gcggctgtaa ccgaacatcc gtggctggca attctgctca gtgccgtact
     4321 ggccggctgg ttgatggggt tgatgtcctg gctggtagcc gcctcgcgcg acacgatcag
     4381 ccaaatagtc gtggtctggc tggtcgccac ggtaatcggt ttcacccagt tgcaccacgc
     4441 cattgtcggc tcggtcgaag tgctggccgg cgtgttcgcg ggacgcgcca cactcgcgtc
     4501 attcggacac tttctgttgt gggccaccat tggaaacgcg atcggcggcg gagttttcgt
     4561 cgcagtgctg aaatacagcc acgccacacg cgaggaaccg gcagatgtgg ggtaaagagc
     4621 agttcatgaa catggccgaa ccggacgcga agttgatgca agccatgctg cgaattgatg
     4681 cgctgctgca acgcgtcgaa cagcttcccg accccgccgt tcgcgcgaac gtcaccgaca
     4741 tccttcagag cctgctggat ctgcacggcc aggcgctggc gcggatgctc gaactgttcg
     4801 gccggcgtgg tgatcaagcg cagccgattt tcgacgcact cgaagcggac cagctaatca
     4861 gcggcgtgct gctggtacat ggcctgcacc cgcgcagcat cgagcagcgc gtccagcgag
     4921 cgctgcaaca aattcagccc tacctcgcgg cacataatgg aggcgtggaa ctgctggaag
     4981 ttaccggcga gcaggttgtt cgtctgagac tggcaggcga ctgccgcgcc tgccccggtt
     5041 cccgcgcaac gctggacaat ctgatcaaag aggccattta cctcgcggcg cctgacgtgg
     5101 ccgccattga agtcgaggga gcggcccccg acacagacgc cgcggcggcc tcagcgccac
     5161 cgaaggggca ggccgagcgc gaatgcccac gcgtcgcatc gccgttttcg cattcatcct
     5221 tcaacgcaga gcagaggtct tgaatatgtc tccgagaaag ttgtcgtaca aagatccccc
     5281 ccggcccgtg cacgtgtcgg ggaccaacaa gggcgaggag cttgtgcaac agaagggccg
     5341 cgagcccggc cgcaacgagc ccggcattcg cccatatcgc acagcgcgtg actcgactgg
     5401 cttgaacgcc gcgtcgcacg cgccaatcga cgcgcgcatg ccggaaatcc cgccggcctg
     5461 accgatggac gcactcccgc caggtttcag gctggcggag caggatatgt tgagcagccg
     5521 cgtacaggag cacgcatgcc tgatctagac tttgccgttg aaggcgcgag tgtggaacca
     5581 tatgcggcgg cgccgctgct gctcttgaaa gtgcgcgcga ccgaccgcga cccgcaaccg
     5641 ctgccgattc acagcgtgtt gctgtacagc caagctcgca tcgagccggc gcagcgccgc
     5701 tattgccctg ccgaacaggc acatttgcgg gaacttttcg ggtttccgga acactggccg
     5761 cggagtttgc gaagcctgct gtggacgcac acacaatcaa ctgtgcccgg attcacgggc
     5821 ggcacgctgt tcgacctgcc ggtgccatgc tcatcggatc tgagcctcgc ggtgaccaag
     5881 tactgcaatg ccttggagaa cggcgagctg ccgctgttgc tactgttcag cggaacggtt
     5941 ttctacgccg cgccgcacgc cccgctgcaa gcggctccga tcggccggga tcgcgaggcg
     6001 ccgtttcgcc tgccagttcg cgtgtggaaa gacctgatgg accgctatta cccaaacagc
     6061 gcctggctgt gtctgcggcg cgacctgatt gaacggctac aacactacaa gctgcgggcc
     6121 ggcctgctca gttgggatga gacgctggaa cgactgctgg acgcggagca ccgcgcgcat
     6181 ccggcgcccc ctgccgttcc agaaccgctc gctggcgcgc cggtgaccgc ggaagtgcaa
     6241 tcaaaggctg tcaaagcagg agcgccggca tgagcgattg tcagtccgga cccacgaacg
     6301 gcggccggcc cgtcccaatt aacagtcgcc atcccgcgac ggatgattcg aagctacaaa
     6361 gtgcggtcgt caacggcatt gaagtggctg cgggcgaccg tgttcgcctg cgcccgcagc
     6421 agcgcgccga cgccgtcgac ttattgctcg tgggaagaac ggccaccgtg gtctcgattg
     6481 agcacgattt cgagggccgg caatatctgg ccgtgacggt cgacgacgac cccggccgcg
     6541 acctcggccc cgctggttgg cccggccacc gcttcttctt ccggcccgac gaactcgagc
     6601 ccctgccgtc gcagccggga ggggtgccat gacgccgcgc attcttgtag caggcatcgg
     6661 caacatcttc ctgggtgacg acgcgttcgg cgtcgaagtg atccagcggc tggcacaacg
     6721 cacctggccc gagggcgtgc gaattgttga cttcggcatc cgcggcttcg acctggccgc
     6781 cgcactgtta gagggctggg attacgcgat cctcatcgac accgccccac gcggcgccgc
     6841 cccgggcacg ctgtacgtga tagagccgga actggaccgg gaaggcgagg ctcctgctga
     6901 gccgcgaagt gaggccggaa attccgctga gtcgcggcgc ggcggggcct tcgccctccc
     6961 ggcaatcgca cccgccggcg cgcttgacgg ccaccgcctc gaccccgtgg gcgtgctggc
     7021 actggttaaa ttgctgggag gaccattgcc gcgaatactg ctggtcggct gcgaaccagc
     7081 ccggggcagc ggcaccgatg aggtaaccgc ggagttgagt gcgcccgtcc gcgctgccgt
     7141 tgttgaaagc atcaaactcg tggaatcgct aatagccgcg ctccgccggc ccgatccgcg
     7201 gagccagtcc gcattcactg ccctgttcca agggagaact gaacatggtt gccatccgca
     7261 cgaagcccat gcggcgccga gttagagaga cgaccgacgt tgtcaccgcc cacccgggcc
     7321 tgactgtggc ggccatcatc ctcctgagca tcggcggcgc catattcgtc gcgatgtacc
     7381 cggaaatgaa gcgctacctg ctggccaagc gcatgtgaac atccccaatg cgcggatgcc
     7441 gggcgggagt ggattgacgg cgcgcagcat tttcacgcag cgttctgcgc gcgagacgct
     7501 gcggcgcgct tggccgaggg catgcccgcg cttacagctt gggcatggca cccgcgcctc
     7561 gccgttctgt cacgcaccgt cgattctgcc gcggacccgt cgattcttgt cgcggaccat
     7621 acttgccccg tcagcaggcg cgatttagat tgcacctcga agcgcgcgag cttgcgcctg
     7681 gaggcgcgcg aggcgtgcgg aacggacggc tccacaccgc ctacgacttg atgaccagga
     7741 ggttgtatgt attccagtct cagtgacgac tccgccaaga cgcccaccgt aacccgccgg
     7801 cagttcgtgc aaatcggagc cgtcggagcc ggcgtgctcc tgggtacgcc gcacctgtgg
     7861 gcccagcaag cctcgcagcc gtcgccgcct cccaaaccga aaaccaatat cgatgacgcc
     7921 ctgaaagtcg cgcgaaccaa atactcgctg cccggccttt ttcccggcaa agtcgtgcac
     7981 gtgcaggacg cgcaggccgt aaccgatggc cgcatcgtcc ccagcgcagt ggaacgcatg
     8041 ttcgcgcgcg gcctgcagga gctcaccggg cagaactcca gcgagagctt caacctgctg
     8101 ttcaccaagg atgacgtggt cggcatcaag gtgaacccgg ccggggcggg catcatcagt
     8161 acccgcccgg aagtcgtcga tgtagtcatt gcctggctgc tgggcaacgg cctgcccaag
     8221 cagaacatca tcatctggga ccgcttcgac atgatgctca aggacgccgg ctacacgccg
     8281 gagcgcttcc ccggcatcaa gatcgagggc ttgcagacta tggtcgagaa gttgccggag
     8341 ggcgataacg ccgaccacag cgcctggctg gacaaggacg gcaatcacat cagcgccggc
     8401 aacttcgacc aggatgtgta ctactgggcg gacgtggagg ggccgaagga cctgccgtac
     8461 ctcaatcagc atgtcttcaa cggcaagtat tcctacttcg gcaagcttgt gacccaaaag
     8521 ctcaccaaga tcatcaacat accggttttc aagaacaccg gaaacggcgt ttcgatggcg
     8581 accaagaacc tggggtacgg cgccatatgc aacacgaatc gcctccacac gccgctgttc
     8641 tttgacgtct gcaccgaggt gctggcgttt cccgcgctgc gcgacaagct ggttctcaac
     8701 atcatcgatg gcctgcgcgg gcaatacgaa ggcggcccca tgccggccgc ccagttcgta
     8761 tacgactaca actccctgtt ctttgcgact gatccttttg ccgccgactc agtgggccac
     8821 gaattaatgg tgcagaagcg taaggaaatg ggaattaagg tgaacgagca cccgcggttc
     8881 accgagtatt tgcgttacgc cgaacggctc ggactgggcg tggtcgaccc ggctaaagtc
     8941 caggtacggc atgtgtaatt ggatgccgaa ggccgcacgg acatttggac tggcgctggc
     9001 cttgctgccc gtcctggcgc tagccgcctg tcgccccgct agcgcaccgc ccatgattac
     9061 agcggccgcc gattccacac ctcctgcgac gtcgtccgct ccaacgcccc agcgcgaaag
     9121 cagcgaatta cagtggacgg aagtagagcg gcggcaattc gtcggccagg acctgttcaa
     9181 tcacatagac ggcgcggcgg aagtctttct ggagatgggc ttccgcgagg tgctcgttcg
     9241 gctatacagc aacggacagg ccaaactcgt ccacgaagtc tacgagatga aagacccgac
     9301 ggccgcgcgc ggcatttacc tgcgcttccg cggccgggga acgcctgtcg caggcgtcag
     9361 cgggcggaac ttcggtaacc gctaccagat cggcgcccag aaggaccgct acttcgtcca
     9421 gatcaccgat gcctccggcc ggggcgagtc tctatcagcc atgacgcggc tggtcaacca
     9481 ggcgctggcc gcgatcccgc atgatggaga cgtgcccttg ttggacttgc tgccggccga
     9541 gggtctggtg ccaaactccg aggtcatcgt ctgcggacca tactcgttgc agagcgtctt
     9601 tacacttggc gaaggcgata ttctccaact gcaaggacaa aggtgcggga tcgccggcga
     9661 ttatgtgacc gaacacgagg gcagcgtcac gcgtctgatc gtcccgtacg gcagcgccgc
     9721 ggctgccggg acggcgtttc aacacctgct gcacgggctt gactctgaac tccaggtact
     9781 ccaccggaac gagcacaccg ccgttttccg cgagaagaac ggccagttcg ggagcatcac
     9841 cgcccgggac gacaagctcg acattcgcct gcacctggtt cgtgaccccg gcctggcgag
     9901 ccagccaacg gttccgtgac cacccgaatc agtcgcctca tgcgcttgcc gtgcgcttgg
     9961 gcttcggaca aatggcgcag actgcggccg cggcgcttag ctccagaaat ggcatcagcg
    10021 gtacgcggta gcgcaggctg ccgatgaaga tgcagtgcag cagcgtgaaa tacactatcg
    10081 gcagccagac cagcgtttgg tatctaacgc cggtccgcga gcggcgaacg aaggcggttc
    10141 gccagatcgc aacggcggcc agaagaagca caagcgtggt gaaaagtgcg ctggcgatcg
    10201 cggtcagccc gccgcggtaa gcctcaaagt tcggcaccgg gttccacgta cgcaggaatt
    10261 tgacaccggc caggtgcacg acacgggccg gatcgcgttt catttgctcg agcgccttgt
    10321 cgcgcagcaa ccgatctcgt tcggcttcgc cgaggccgct gagtcccggc attttttcga
    10381 ggaactgttg attgctgcta ccgtcggcct gcggcccctg ggcgtcgtag agcgttacac
    10441 cgccgttcgt ggagagccag gcatacgagc cgatggcgtg gtggtttcgc aggccccagg
    10501 gcaacagaaa cacgcccagc gcggcagcgt acgcgactac acatagagcg gcgcgacgct
    10561 tgttccgatc gtataaccac agaagcagcc aaagcgagat tatccacccc agggccgacg
    10621 ggcgggtgag gatggccgcg ggaccgagga ctgcaaccgc cagagcagat ttgcggaacg
    10681 ttgggctgga agagggatca tccacaaaag agtgatcgtc cacaggagcg gcatcgtcgc
    10741 gtcttccgtc cccattaaca ggtctgatga gtcgccaggc ggcggccgtc aacgccacgg
    10801 caatcagcgt gaagaggacc tcggaaagca gcaggttggt gaagaagatg ccgtacgggt
    10861 caatgcacgc cagcccgccg gccaataatg ccgggcgcgg accgagggcg tcccgggcca
    10921 gccggtagat cagccagacc gttgcggccc ctagcgctgc ctgcgccagt cgcgcgaaaa
    10981 gaacgccggt ctggcccgcg cccgcgaaga tcgccaaaaa gagcggatac agtggcatgc
    11041 gcgcggcgaa gcggccgtcg tcagtgacca tctggccggc gtcgaccagg tttgaagcca
    11101 tcgcccaatg cagacgctcg tcgtcgaact cgaacgccgg ccccgtacga tgccattgga
    11161 agccgaccca gccagcgcgc agcagcagcg ccagaatgaa cagccacaga cctgctttgc
    11221 tgtgggcaga attcagtccg ggtgcgactg tcatgtcagt ccgggtgcca ccgtcatttc
    11281 agcccaggtg cgaccgtcat gtcacttgcc ggttcttcgc cgggcgtagc gtaacgcgca
    11341 gcgcccgctg ggtcgcgggc gtcagcttga ctcgttcgtc gattctcagc ggcatgatcc
    11401 agaccggccc atcctgatcg cacagcaagc cggttcggct gcgtgcggcc ggctcgactt
    11461 tctcgtcgat gaggaaatcg ctgaccgact ttgcgccggg ggcgccc
//
LOCUS       DAOSYS010000105        11498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4783_length_11498_cov_5.335839, whole genome shotgun sequence.
ACCESSION   DAOSYS010000105 DAOSYS010000000
VERSION     DAOSYS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_11498_cov_5.335839"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLU99_08070"
     CDS             complement(<1..534)
                     /locus_tag="PLU99_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO17051.1"
                     /translation="MFGTSRVPRTGGVLLVCNHQSFLDPVLATLALPRECHYMARDSL
                     FHNPFFRRLIESLNAFPIKRGTADLGAIKETLRRLKAGALVVTFPEATRTPDGSLQPM
                     APGVILLARKARVPLVPAAIMGAFESWPRHARLPRPHPVVIAYGQPLSPDTLANLPDD
                     ACIAEVRRRIQELMDRFG"
     gene            complement(650..1360)
                     /gene="cmk"
                     /locus_tag="PLU99_08075"
     CDS             complement(650..1360)
                     /gene="cmk"
                     /locus_tag="PLU99_08075"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOO17052.1"
                     /translation="MIITIDGPAGSGKSTAARKLAARLGIPYLDTGAMYRVVTLAALE
                     AGIDLSDEARLAALASGDDYQLDLGPTHIRVTLRGRDVTEEIRSMRVNDNTRHVAALP
                     PVRQALVARQRAIGRRLGSLVTEGRDQGSVAFPDADVKFFLDASPEKRAERRLHELLA
                     DGEDVTFEQVLANLSTRDRTDSQRQAGPLVVPPGAIRIDTTQLSIAQVLDLMTEHVRR
                     AGFSPPSTASANPVGGGH"
     gene            1672..2661
                     /gene="galE"
                     /locus_tag="PLU99_08080"
     CDS             1672..2661
                     /gene="galE"
                     /locus_tag="PLU99_08080"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOO17053.1"
                     /translation="MRVFVTGGAGYVGSHCVKRLLAGGHEVSVFDNLSAGHREAVDPR
                     ADFVQGDLSDKARLADLLRPGRFDAVMHFAGSLDVGESVQRPLEYYRNNVVNTLNLLE
                     CMRDSDVRRMVFSSTCAVYGEPERLPITEDLPKSPISPYGASKLTVEYMLEHSARAWG
                     LGSVALRYFNASGAASDGSIGEDHSPEIHLVPLVLQVALGQRDKIKVFGDDYPTPDGS
                     CIRDYIHVEDLADAHLLAIEGITEGRKEAYNVGTGKGTSVFEIIEVARAVTNHPIPAE
                     VTARRPGDPPGLYANSDKLRERFRWAARYTDIRDIVASAWDWHRSHPHGFRSN"
     gene            complement(3270..3506)
                     /locus_tag="PLU99_08085"
     CDS             complement(3270..3506)
                     /locus_tag="PLU99_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17054.1"
                     /translation="MEKKNPACLLGHAGFCKAPHRSTQRPVANRADERLSLDVPLFHG
                     TPSFAQFGIIAAHIPNVLKKDLGCLAYHPTEGTL"
     gene            complement(3565..4341)
                     /locus_tag="PLU99_08090"
     CDS             complement(3565..4341)
                     /locus_tag="PLU99_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17055.1"
                     /translation="MLRNSLLLVLAVSVAAPLFAQTPQRMRVPGEKIKTIGPATEKAE
                     QPKPVPVEQKLRYIVKRLDLNEKQKQHAEGLFAILQSESNLSTEETKDRMEVIMSTYR
                     AMQDAEKAGDKDRADQLREELKGLAPGAAAERNFVNGLMPVLDDKQKEKFQALLKKYE
                     KATDLSLKPIEVIRLAREQNLTPEQAQQLDKIQTEFRQSVAAGTAPDAQALEKLISDV
                     SGILNPTQLAAFQKEVAERRVDEPTPPSTQPAADPSASHR"
     gene            complement(4358..4486)
                     /locus_tag="PLU99_08095"
     CDS             complement(4358..4486)
                     /locus_tag="PLU99_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17056.1"
                     /translation="MNRRVAGSAIQCVARGLRNSAWGVDFAGSAAPFAVQVTLYVA"
     gene            4642..5148
                     /locus_tag="PLU99_08100"
     CDS             4642..5148
                     /locus_tag="PLU99_08100"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOO17057.1"
                     /translation="MPVLIDGDNLLHAARAAKPPGPLVGRSILCDTIGRWARRRAERV
                     RVIFDGPEPNPSLAKQIGNADIDVSYSGGQSADAVLIGILETDSAARRLLVVSSDHEI
                     AHAAKRRRARPIRSDEFWALVQHDLARPPPGPSEPREKRHGQTPEATDKWLREFGLED
                     SQQTTAEG"
     gene            5192..5602
                     /locus_tag="PLU99_08105"
     CDS             5192..5602
                     /locus_tag="PLU99_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO17058.1"
                     /translation="MIECQAQPSAAALAETPERRAEPRQQVVGSLWMVDHHGSTVLRC
                     QCVEASRSGLRLRVPPGYGVVEGLRYELRSHLPGAQPLTEGFDLTGSRWATVVRVQFR
                     VNDHSDYLDVGVMFDNTDAPVLSIAGRSLAKAVV"
     gene            complement(5586..7052)
                     /locus_tag="PLU99_08110"
     CDS             complement(5586..7052)
                     /locus_tag="PLU99_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOO17059.1"
                     /translation="MNPLLDAFEKVCAERGGALAAGDQTVAFNYQTLRAVAAGLAEQI
                     EARTRQSQVGILAPTSAVGAAAIFACWYAGKVPVPLNFLLSPAEMGRIIRDAELDFIL
                     TVPHFQAAAAATGLNHLVLTPESLTPAQAMRPVDRSRRDASADDVAAILYTSGTSGDP
                     KGVCLSYENLTRNAQACIEHAHIRPDHVWLSPLPQFHTFGFTVLTVTPLLLGSTVWYL
                     PRFSPAALAQMVAEKKVSVLIAIPSMFAAVLNLKSAQREMFQTLEYTISGGEPLPEKV
                     YRGFLERFGVAICEGYGLTETSPVVSMNMPWDNRPSSVGRLIPGVSVTVVDEHGAELP
                     AGQTGELVVRGHGVMLGYYHNPEATAEVLRDGALWTGDMGHVDADGFVYITGRAKEMI
                     IVGGENVYPREIENVLAEHPAVREAAVIGVRDDVRGELPAAFVILKEDAVANETELRD
                     FCRGRLAGYKVPRWVRIAPELPRSATGKILKRALTPPP"
     gene            complement(7300..8400)
                     /locus_tag="PLU99_08115"
     CDS             complement(7300..8400)
                     /locus_tag="PLU99_08115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO17060.1"
                     /translation="MSDKVRRVALVLVLAAAVQACPASEPAPASAPAGTFRPDMVAAR
                     QLYDPNRPILVRFILQNTSDVPVEIPVTTTADLDGAIALPLEIVFGSADNPALSIAYR
                     EEKAVHVPPPTPVGSPLNLRESVLRLAPAAFIGAEVDLREFYPGLRYSGTYHLEWRPA
                     GGSGGVATLDFRVEARKDAIVVTDRGKLTFTLAYDQAPRNVENFLDLVRQGFYDGKSI
                     HRIVPNFLIQAGCPKGDGTGIRPDGRLVPAEFNDLPIQLGTLVMARKPSDPNSASCQF
                     FVALARLPELDGQYTVIGQASDVESMQTLSTIAAEPTDSRDHPRMPLVIRSINLVDAE
                     VTGVTRFDLKPTRRERPAPPPTPAQQASAKTE"
     gene            complement(8504..9703)
                     /locus_tag="PLU99_08120"
     CDS             complement(8504..9703)
                     /locus_tag="PLU99_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO17061.1"
                     /translation="MKILVACELPEFAIAELRTLTTEIVHRPDARPDELRELIPGVGI
                     LVVDGRRVSPDTIQRGEALQMIVHAGPGPGDIAVADASAQGVFVTNCPDQHAVAVAEL
                     AFGLILALDRRIVDSTVALREGRWNRNEAKDAHGLAGRTLGLLGAGPACREIAARAQA
                     FGMPVIAWCPASNADDHASNIRLCTSARELARTSDIVVVTSLANGQEARLLADADFLE
                     NMKEGASFVHVGGPGAVDEAALAKITPVRKLRVAADVFSSEPSTDTGRFRSRLCELPG
                     VIGTPHVAAVTQQAQNSTAAEVIRIIRSFLVSGEALHCLNLLEHSPATWQLMLRVRDA
                     VGVMASILEAIRADGINAEEISSRVFVGARAAWCTIALDERPSTEALEAIRALHDVLY
                     LEIRAVV"
     gene            complement(9819..10559)
                     /locus_tag="PLU99_08125"
     CDS             complement(9819..10559)
                     /locus_tag="PLU99_08125"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOO17062.1"
                     /translation="MRPLFVFRDPPLDGATNMARDEHLLHSEELRPGALRIYYWSPPT
                     ISLGYFQKFAAVAELPEDVRGLDVVRRPTGGGAILHDQEITYCIAIDESLSIAHRPPA
                     ALYHLAHECWREAVAADAPGLEMAPDSAPFPSPRSGPFFCFEQPGRTDLVLGGAKLLG
                     SAQRRARGRVLQHGSLILGRRFDAHPGAHLNSPPPEVRERWISAFIAGVARALELRAQ
                     PASWTDEQLRDVAVLREKYAGADWTRLR"
     gene            11030..>11498
                     /locus_tag="PLU99_08130"
     CDS             11030..>11498
                     /locus_tag="PLU99_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO17063.1"
                     /translation="MKKHKADGKALVIVESPAKARTINKYLGSGYVVKASMGHVRDLP
                     ERHFGIDVSEDFKPTYDVVRGRTRVVNELKKLAGTAPEVFLATDRDREGEAIAWHLVE
                     ALKLEPEKVRRVIFNEITRAAISEAFQHPREIDLDRVNAQQARRILDRIVGYEL"
BASE COUNT         2167 a         3528 c         3653 g         2150 t
ORIGIN      
        1 gccgaagcgg tccatcagtt cttgtatacg gcggcgtact tccgcaatgc aggcgtcgtc
       61 cggcaggttg gcgagcgtgt ccggcgacag tggttgcccg taggcgatga ctaccgggtg
      121 tgggcgcggc aggcgcgcgt ggcgcggcca ggattcgaaa gcgcccatta tcgccgcggg
      181 aacgagcggc acgcgcgctt tgcgggccag cagaatcacg cccggcgcca taggctgcag
      241 cgaaccgtca ggcgtgcgcg tggcttcggg gaacgtaacc actaacgcgc cggccttcag
      301 ccgtcgcagc gtttccttga tcgcacccag gtctgctgtg ccgcgcttga tcgggaaagc
      361 gttgagcgat tcgatcagac ggcgaaaaaa cgggttgtgg aagaggctgt cgcgggccat
      421 gtagtggcac tcgcgtggca gggccaacgt cgccaggacc gggtcaagga agctctgatg
      481 attgcacacc agcagcacgc cgccagtacg ggggacgcgg gacgtgccga acacgcggcc
      541 tcggaagaaa gccaaaaaca gggtttgaca ggctatacga caaaagcggt accaggggcg
      601 catcggtgcg gtctgggcag gcgccgcgga ttgctcgatg ccgcccattt caatgacccc
      661 cgccgactgg attggcgctc gcagtgctcg gagggctaaa gcccgcccgc cgcacgtgct
      721 cagtcatcaa atccagcacc tgcgcaattg aaagttgggt ggtatcgatc cgaatggcgc
      781 cgggcggcac gactagcggt ccggcctggc gctgcgaatc ggtgcggtcg cgcgtactca
      841 agttcgccag tacctgttcg aaggtcacat cctcgccatc ggcgagcagt tcgtgcaggc
      901 ggcgctccgc acgcttctcc ggtgaggcgt ccaggaagaa cttcacgtcc gcgtcgggga
      961 aggcaacgct gccttgatcg cgcccctcag tcacgagcga gcccagccgg cggccgatcg
     1021 cacgctgcct ggcgaccagc gcctggcgca cggggggcag ggcggccacg tggcgcgtgt
     1081 tgtcattgac gcgcatggaa cggatttcct ccgttacgtc gcgtccgcgc agcgtgactc
     1141 gaatgtgagt ggggcccagg tcgagctggt agtcgtcacc gctcgccagc gcagccaaac
     1201 gggcctcgtc gctcaaatcg atgcctgctt caagggcggc cagcgtaacg acgcggtaca
     1261 tggccccggt gtccaggtag ggaatcccca gccgcgctgc cagcttgcgc gcggcggtgc
     1321 tctttcctga accagccggg ccatcaatag tgatgatcac tgccgcaccg ctgccttaaa
     1381 tagccgcacc gctgccttaa tacccgattg cggggggatt atacggagag gtgggcggcg
     1441 cggcgaggca actcaaagac cggcttggcg gggtcgacgg cagatcaagg ccgccggtcc
     1501 gaaaaatgtc tgcggtttca tttgacgcgg cccgcgcagg ggggatgatt catccgtgcg
     1561 tctgccgcgc ggggccggcg cgaacaggtt gccctacgtc ggccacgggg ggccggcgct
     1621 acaggattga cgctacaggc ttaaggatcg cacgatatta aggagcacac gatgcgcgtg
     1681 ttcgtcacag gcggggcggg ttacgtcgga agtcattgcg tcaagcgttt gctcgcaggg
     1741 gggcacgaag taagcgtgtt cgacaatctc tcggccggcc accgcgaggc ggtcgatccg
     1801 cgcgccgact ttgtccaggg cgatctgtcg gacaaggccc gcctggctga tttgctgcgg
     1861 cccggtcgtt tcgacgccgt catgcacttc gccggctcgc tcgacgtcgg ggaatcggtg
     1921 cagcggcctt tggaatacta ccgaaacaac gtcgtcaaca cgctcaatct gctggaatgc
     1981 atgcgcgact cagacgtgcg gcgaatggtg ttcagcagca cgtgcgcagt ttatggcgag
     2041 ccggaacggc tcccgatcac cgaggacttg ccgaagagtc ccataagccc atatggcgcc
     2101 agcaagctga cagtggaata catgctcgag cattccgcgc gcgcctgggg tttaggctcg
     2161 gtcgcgttgc gttacttcaa tgcctccggc gcggcctcgg acggatcgat tggcgaggac
     2221 cactcgccgg agattcactt agtacccctc gtactccaag ttgccttggg acaaagagac
     2281 aaaatcaaag tcttcggcga cgactatccg acacccgatg gctcctgcat ccgcgattac
     2341 attcatgtgg aggacctggc agacgctcat ctcctggcga ttgagggtat cacggaaggg
     2401 cgcaaggagg cttacaacgt cggaaccggc aagggtacga gcgttttcga gataatcgaa
     2461 gttgcgcggg ctgtgacgaa tcaccccatt ccggctgaag tgacggcgcg acgtccgggc
     2521 gatccgccgg ggctatatgc caattccgat aagttacggg agcgatttcg ctgggcggcg
     2581 cgatatacag acattcgcga cattgttgct tccgcatggg actggcaccg ctcccatccg
     2641 catggattca gatctaatta gaaattagaa aaaaaacagt tgtgcaggac acccggcatg
     2701 tggtatatta cctgtggacg gcttgaggtg gaaacgtcat agcgtattcg attttataac
     2761 gcagatttcc atccgtgcat tctatcctga ttttcttcgc cacctgatgc cgccgacaac
     2821 gtgataacct cgttgtaggt gcggtatcag cggcacccgg agatactccg gacttctgcc
     2881 aggcgtagaa atccccccaa aataagccaa aaacactagc cgaacttaac catggcggct
     2941 ggctgcttgt tcctcctccc agcagcgcgg ccgcccgaga taacagtggg gccgcgccgt
     3001 agcggagcgg tttaaggatc ggcgagtcag ataactgact cgccctgcgg taaaaggagc
     3061 gagcgatgtc ttggccacct aagggactgc tgacgcggat gaagaccagc gaagcatttg
     3121 gtccgccgct gaccccggaa gagctgactg gaaacctctc aaagctccag aatgagctgc
     3181 atgcccgtat ggtatgtccg gccggacgaa accaggttta cgttcgatct ctacttaccg
     3241 gaaccgccac cacacggccg cggattgccc taaagtgttc cctccgtcgg gtgataggcc
     3301 agacacccga ggtctttttt gagcacattc gggatgtgtg ctgcaataat cccgaactgt
     3361 gcgaagcttg gcgtgccatg aaagagcggc acgtcgagac ttaagcgttc atcggcgcgg
     3421 ttggctaccg gccgctgcgt cgatcgatgg ggagccttgc agaacccggc gtgacctaag
     3481 aggcacgccg ggttcttctt ttccatgctc acccttcaca cgtcatatca cctgctcgcc
     3541 tgctcagctt agcagttccg gggatcaacg gtgggatgcg ctcgggtctg cagccggctg
     3601 agtgctcggc ggggtgggct cgtcaacgcg gcgctccgcc acttccttct ggaacgcggc
     3661 tagctgtgtc ggattgagaa tgcccgaaac gtcgctaatc aacttctcca gggcctgggc
     3721 atcgggcgcc gtgccggctg ccacactttg ccggaactcc gtctggattt tgtcgagctg
     3781 ctgggcctgt tcgggggtga gattttgctc ccgggccagc ctgattacct cgatgggctt
     3841 tagcgacaaa tcagtcgctt tttcgtattt cttcaacaac gcctggaact tctctttttg
     3901 cttgtcgtcc agtaccggca tcaggccatt gacgaaattc cgctcggcgg cagcgcccgg
     3961 cgccaggccc ttcaactcct cgcgcagttg atcggcacgg tccttgtcac cggccttttc
     4021 cgcatcttgc atggcgcgat aggtcgacat gatcacttcc atcctgtcct tcgtctcctc
     4081 cgtgctgaga ttcgattcag actgcaggat ggcgaacaga ccttccgcgt gttgcttctg
     4141 cttctcattc aagtcgagcc gcttgacgat atatcgcagt ttctgctcga cgggcaccgg
     4201 cttgggctgc tcggcttttt cagtcgcggg gccgatcgtc ttaatcttct cacccggcac
     4261 gcgcatccgt tgcggcgtct gggcgaatag cggggcggca acggacacgg ccaagaccaa
     4321 aagaagcgag ttgcgaagca tcgaaaactc ccgtggatta cgcaacgtac agcgtcacct
     4381 gcacggcaaa cggagccgca ctaccggcga agtctacgcc ccacgccgaa ttccgcaagc
     4441 cgcgagccac acactggata gccgaaccgg caacgcgtcg attcatcagc gctcgtcgtt
     4501 ccagtgattg cgggtccaaa gtcaaggata cactctcata ctgagccagg accttcctga
     4561 atcttcggta atgacgatct cgcgtcaatt ttgctttgca gctaacctaa ctgtgcgtta
     4621 tgctttatat ggaggcgttc ggtgcctgtc ttgatcgacg gtgacaacct cctccacgcg
     4681 gctcgtgctg ccaagccccc ggggccgctg gtcggccgct cgattctctg cgacaccatc
     4741 ggtcgttggg cccgccgccg agccgagcgc gtgcgcgtca tcttcgacgg accggaaccg
     4801 aaccccagcc ttgccaagca gatcggcaac gccgacatcg acgtcagcta tagtggcggc
     4861 cagagcgccg acgccgttct aattggcata cttgagacag actcggccgc gcggcggctg
     4921 ctcgtggttt cttccgacca cgaaatcgcg cacgccgcca agcgtagacg tgctcggccc
     4981 atacgttcgg atgaattttg ggcgcttgtt cagcacgatt tggcccgtcc gccgcctgga
     5041 ccgtctgagc cgcgcgaaaa acgtcacggt cagacccctg aggcgaccga taagtggtta
     5101 agggagtttg gtttggagga ctcgcagcag acgaccgccg agggttagcg gaggccgctg
     5161 cccatccctc ccagtgcaag gagcccgagc catgatcgaa tgccaggcgc aacccagcgc
     5221 agccgcgctg gccgaaacac ctgaacgtcg agcagaacct cgccagcagg tggtcggttc
     5281 tttgtggatg gtcgaccatc acggatcgac cgtgttgcgt tgccagtgtg tcgaggcttc
     5341 tcgcagtggt ttgcgtctac gcgtgccgcc gggctatggc gttgtcgaag gcctgaggta
     5401 cgaactgcgc tctcatttgc ccggtgcgca acctctgaca gagggcttcg atctgacggg
     5461 cagccgttgg gcgacggtcg tgcgagtcca attccgggta aacgaccaca gcgactattt
     5521 ggacgtaggc gtcatgttcg acaacacaga tgccccagtc ctatcaatcg cgggccgcag
     5581 cctcgctaag gcggtggtgt aagggcgcgc ttcagaattt tgcccgtagc gcttcgcggc
     5641 agctcgggcg cgattcgcac ccaacgcggc accttgtacc cggccagacg accgcggcag
     5701 aagtcgcgca actctgtttc gttcgcgacc gcatcctcct tgagaatcac gaatgcggcc
     5761 ggtagctcgc cgcgcacgtc atcgcgcacc ccgatcaccg cggcctcgcg cacggcgggg
     5821 tgttcggcca ggacgttctc tatctcgcgc gggtagacgt tctcgccgcc gacgatgatc
     5881 atctccttcg cccgtccggt gatgtagacg aacccgtcgg cgtccacgtg gcccatatcg
     5941 ccggtccaca gtgcgccgtc gcgcaggact tcggccgtgg cctccggatt gtgatagtac
     6001 cccaacatga cgccgtgccc gcgcaccacg agttcgccgg tttgccccgc gggcagctcc
     6061 gcgccgtgct catctaccac tgtcacgctg acgcccggaa tcagccggcc gactgagctg
     6121 gggcggttgt cccaaggcat gttcatggac accactggcg aggtttcagt caatccgtaa
     6181 ccttcgcaga tcgccacacc gaatcgctcc agaaaaccgc ggtatacctt ctcgggcaag
     6241 ggctcgccgc cgctgatggt gtactcgagg gtctggaaca tctctcgctg cgcactcttg
     6301 agattgagta ccgcggcgaa catgctggga atcgcgatca gcacgctgac cttcttctcc
     6361 gccaccatct gcgccagggc cgccgggctg aagcgcggca agtaccacac cgtcgagccc
     6421 agcaatagcg gtgtgaccgt caacacggta aagccaaagg tgtgaaactg cggcaggggg
     6481 ctgagccata catggtccgg gcggatgtgc gcgtgctcga tgcaggcctg tgcgttgcgg
     6541 gtcagatttt catagctcag gcagacgcct ttgggatcgc ccgatgtgcc ggaggtatac
     6601 aggattgccg ccacgtcgtc cgcgcttgca tctctgcggg agcggtcaac cggccgcatc
     6661 gcctgcgccg gcgtcagtga ctcgggcgtg agcacaagat gattcaagcc cgtcgctgcg
     6721 gctgccgcct gaaagtgcgg cacggtcagg atgaagtcca gttcggcgtc gcggattatg
     6781 cgtcccattt cggctgggct aagcaaaaag ttcagcggca ccggcacttt cccggcatac
     6841 cagcaggcga atatcgcggc cgcgccgacg gctgaagtcg gcgcgagaat gccgacctgc
     6901 gattgccgcg tccgcgcctc aatctgctcc gccaggccgg cggcgacggc gcgcagcgtc
     6961 tggtagttga acgcgacggt ctgatcgccg gccgccagtg ccccgccgcg ctcggcacag
     7021 actttttcaa acgcatcgag aagcggattc atacggaccc ttcgtctgga ccttaccgga
     7081 tattcgagat tctaagcgaa tgtgaacggc aaatcagcgc cagtgataaa gtgataaggt
     7141 gatagagtga taaggggttg cgtggaccgg actttatcac tctatcacgt tatcacctta
     7201 tcacgtcatc acgggtgcac atggtccccg gcactgcttg aagctgcgct tcaagcagtg
     7261 gcacactttg gcttgtctcc aagcgatggc gcctttggct tactctgttt ttgccgaggc
     7321 ctgctgggcc ggagttggcg gaggcgcggg gcgttcgcgc cgggtgggct ttagatcgaa
     7381 acgagtgacg ccggttactt ccgcatcaac caggttgatc gagcggatca ccaggggcat
     7441 gcgcggatgg tcgcggctgt cagtgggctc ggccgcaata gtgctgagcg tttgcatgct
     7501 ttcaacatcg ctggcctggc cgatcacggt gtactggccg tccaactcgg gcaggcgcgc
     7561 cagcgcgaca aagaactggc agcttgcgga attcgggtcg gagggcttgc gggccatgac
     7621 cagcgtcccc agttggatgg gcaagtcgtt gaattcggcg ggaacgagcc gcccatcggg
     7681 acgaatgcca gtgccgtcgc ctttagggca gccggcttga atcaggaaat tggggacgat
     7741 gcggtgaatg gacttgccgt cataaaagcc ctggcggacg agatccagga agttttcgac
     7801 attgcgcggg gcctggtcgt aggccagtgt aaacgtgagt ttgccccggt cagtgaccac
     7861 gatggcgtcc ttccgcgctt cgacccgaaa atcaagcgtg gcaacgccgc cgctgccgcc
     7921 tgccggccgc cactccaagt ggtaggttcc cgagtatcgc aggccggggt agaactcgcg
     7981 cagatcgact tcggcgccga tgaacgccgc cggagcgagg cgcaagacgc tctcgcgcag
     8041 gttgagaggc gagccgaccg gcgtcggcgg gggcacgtgg acggcctttt cctcgcggta
     8101 ggcgatggac aaggcgggat tgtcggcgga cccgaagacg atctcgagcg gcagcgcgat
     8161 ggccccgtcc aaatcggccg tcgtggtcac cgggatctca accggcacgt cagaggtatt
     8221 ttgcaggatg aagcgtacca gaatcggacg gttgggatcg taaagctggc gggcggcgac
     8281 catgtccggc cgaaaagtgc ctgcgggggc gcttgccggg gccggctcag acgccgggca
     8341 ggcttgaacc gccgcggcca aaaccagcac caaggccact cttcgcactt tgtccgacat
     8401 caaaccctct ggttctttcg cctagccagt agcgtacaaa cccttatacg ccttgcggcc
     8461 gcgccggttt cccgcggccg ccaatgctgg cccgctccgc ggattaaacg accgcccgaa
     8521 tctccaaata taacacatcg tgcaaagcgc ggatagcttc caaggcctcg gtgctggggc
     8581 gttcgtcgag cgcgatggta caccaggccg cccgggcccc gacaaacacg cggctggaaa
     8641 tttcctcggc gttgattccg tcggcccgaa tggcctccag aatcgaggcc atcacaccca
     8701 ccgcgtcccg tacgcgcaac atcagttgcc acgtggccgg actgtgttcg agcaggttga
     8761 ggcagtgcag cgcctcgccc gaaaccagga acgagcgaat gatccgaatg acttcggccg
     8821 cggtcgagtt ttgtgcctgc tgggtgaccg cagccacatg tggagttccg ataacgcccg
     8881 gtagttcgca gaggcgtgat cgaaagcggc cggtgtcggt gcttggctcg gaactgaaca
     8941 catccgcggc gacgcggagc ttgcgcacgg gcgtaatctt ggccaacgcc gcctcgtcaa
     9001 ccgcgcccgg tccgccgacg tgtacgaagg acgccccctc cttcatgttc tccaggaagt
     9061 cggcatccgc cagcaggcga gcttcctgac cgttggccag tgaagttact acgacgatgt
     9121 cgctggtgcg cgccagttcc cgggccgagg tacacagccg gatgttgctc gcgtgatcgt
     9181 cagcgtttga ggcggggcac caggcgatta cgggcatgcc gaaagcctgg gcccgcgcgg
     9241 cgatctcacg gcaggcgggg ccggcgccga gcaggcccag tgttcggccg gccagtccgt
     9301 gggcgtcctt ggcctcgttt cggttccagc ggccctcacg aagcgccacc gtgctgtcca
     9361 cgatgcgacg atcgagggcg agtatcaggc cgaaggcgag ttcggccacg gcgacggcgt
     9421 gctggtcggg gcagttggtg acgaataccc cctgggcgga ggcgtcggcg acggcgatgt
     9481 caccgggacc ggggccggcg tgcacgatca tctggagcgc ttcaccgcgc tgaatggtgt
     9541 caggcgatac gcgtcgcccg tcgacgacta atattccaac gccggggata agctcgcgca
     9601 attcgtcggg ccgggcatcc gggcggtgaa cgatttcggt tgtgagggta cgcaactctg
     9661 caatcgcaaa ttccggcagt tcgcaggcga ccagaatttt catacggctt ctccagggtc
     9721 cggtcggagg aaaagcggcg gcgcggcgcc ggggcgtatt agaagcgcaa gggggattct
     9781 tctcaacggg caggatgggc gaaggcatac gagcacgctc aacgcagccg cgtccagtct
     9841 gcgccggcat atttctcgcg caacactgct acgtcccgaa gctgctcgtc tgtccaagac
     9901 gccggctgcg cccgcagttc cagcgcccgc gccacgcctg cgatgaacgc gctgatccac
     9961 cgttcgcgca cctcaggagg cgggctgttc agatgcgccc cgggatgtgc gtcgaaacga
    10021 cgccccagga ttagcgagcc gtgctgcaac acacgcccgc gcgcccgccg ctgcgcgctg
    10081 cccagcagct tggccccgcc cagaaccaaa tccgtccggc ccggctgctc aaaacagaag
    10141 aacggcccgc tgcggggcga cggaaagggc gccgaatcgg gggccatctc caggcccggc
    10201 gcatccgccg ccaccgcctc gcgccagcac tcgtgcgcca ggtggtagag cgctgccggc
    10261 ggtcgatgag cgatcgacag gctctcgtcg atggcgatgc agtacgtgat ttcctggtcg
    10321 tgaagaatcg cgccgccgcc ggtgggccgc cgaacgacgt ccaacccgcg cacgtcctcg
    10381 ggcaactcag ccaccgcggc aaacttctgg aaatatccga ggctgatggt cggcggcgac
    10441 cagtagtaga tgcgcaaagc gcccggccgc agctcctcgg agtgcaacaa gtgctcgtcc
    10501 cgggccatgt tagtcgcccc atcgagcggc ggatcacgga aaacgaacaa cggtcgcacg
    10561 gcgacagcat acagcgccgg cttcccgcag cccaccgctt ttcagatgtt tgctgtcgcg
    10621 ggtccggtag ggccctgggg cccagcggac ggcggggagg agggccgccg agaccgtccc
    10681 ggaatacttt atgccggacc gcgccagggg ttatttcttc ggccggatca gccgactatg
    10741 cttgacaggt cgcgaccccg gactaaacat ctttggcccg aggccgtcag acgtttgggt
    10801 tacaagagcg cccgcgattg ttgcgacgcg agttggactt gcggcaacgg ctggcgaata
    10861 gcccgatttg gttggaagca ggtaaaaaag acccacggag gcggatgaaa tacggccgcc
    10921 ccggcggcgg ttacgtaccg tcgtagcgtt tttttgggct gcggttgccc gtaattcgta
    10981 gagattcaat agatttagct cgcttcgcct ggctaaaggc cggttactga tgaaaaagca
    11041 taaagcggat ggtaaagcac ttgtaatcgt cgaatcgccg gccaaggcga ggacgattaa
    11101 taagtatctc ggctccggat acgtcgttaa ggcgtccatg gggcatgtcc gcgatctgcc
    11161 agagcggcac ttcggaatcg acgtaagcga agatttcaag ccaacttacg acgtcgtgcg
    11221 cggccgaacg cgcgtggtga acgagttgaa gaagctggcc gggaccgctc cggaggtgtt
    11281 cctggcaact gaccgcgacc gcgagggcga ggcgattgca tggcacctgg tcgaagcgct
    11341 gaaactggag ccggagaaag tccggcgcgt gatttttaat gaaataacgc gtgccgcgat
    11401 ttccgaggcg tttcagcacc cgcgcgaaat cgatctcgac cgggtcaacg cgcagcaggc
    11461 gcgtcgcatt ttggaccgta tcgtcggata cgagctca
//
LOCUS       DAOSYS010000106        11472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4801_length_11472_cov_5.648769, whole genome shotgun sequence.
ACCESSION   DAOSYS010000106 DAOSYS010000000
VERSION     DAOSYS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_11472_cov_5.648769"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1750)
                     /locus_tag="PLU99_08135"
     CDS             complement(119..1750)
                     /locus_tag="PLU99_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOO17064.1"
                     /translation="MRLNLGCPAADGVRLSTDVYLPDGRGPWPCVLIRTPYNNNDPVK
                     KVPLAREFASNGYAVAIQDVRGRYDSEGEWEPFFNEAADGHAAQAWLAEQEFCNGKIA
                     LYGRSYEGFCVWMGAFGHHPAVKAIIPVVALPDPVINVPWWNGSVCWSMITWALLVHG
                     RTNQDVGQFNWESVYNFRPLNRLDEHLGFSSAAWQEWMKHPLKDEYWQRACYMHRMPE
                     LDLPALHITGWYDDDGASTYNNFPNARRLAKSGAEQYVLIGPWPHATNTKTVVQGVDF
                     GPQEVIDINGFILDWLDKQIGGRPESWGDRPRARIFLMGSNDWHDFDDWPPPGTQEVA
                     YYLNSSGRANSLFGDGLLAREMTSGSAFDEYTYDPQHPTPYLYDAGTLQVGGPFDARP
                     VQRRDDVVCYTTEPLSEDLVICGRVFAELWVSSTAADTEFCAMLCDVHENGLARQLCD
                     GNVRLALRNSLEKREPVPPGEVVQVHIDLWATGIRILKGHRLRLQVASAAVPKFAAHT
                     NTLEPPGSAVETVIATNRVYHDRAHASRLLLPVVA"
     gene            complement(1954..3246)
                     /gene="mnmA"
                     /locus_tag="PLU99_08140"
     CDS             complement(1954..3246)
                     /gene="mnmA"
                     /locus_tag="PLU99_08140"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOO17065.1"
                     /translation="MSRNGKVIVAMSGGVDSAVSAWLLKQQGFECIGVFMRNGAQAPE
                     PTECALGEAADACRDSAETSPSSASTFPGVASPGASPSPGASPSVGNSPVGASTLPGG
                     TLPNSSPAQRRRLGQGCCSAADAQDARAIAGRLGIPFYALNFEQDFHQIIDYFVDEYA
                     RGRTPNPCVVCNTHLKFGKLLRYANALDVEFVATGHYARILRRDGQILLARASNLAKD
                     QSYVLFGIDRAALGRCLFPLGDMPDKAQVRSIAAELGLRVHDKPDSQEICFVPGGDYK
                     ELVRQRRPDTQRVGEVRDPAGRVLGTHSGIANYTIGQRQGLGIAVGRPAYVTKLDVLN
                     NVVTLGSREDLLSGGLVAEKVNWLTDPPAVGQPRPADIKVRYMHKPAAGFITVLPGGE
                     VEARFDEPQSAVTPGQAVVFYDGDIVLSGGWISRAGTG"
     gene            4783..7242
                     /locus_tag="PLU99_08145"
     CDS             4783..7242
                     /locus_tag="PLU99_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17066.1"
                     /translation="MSKCPRLLRLAGIAAGFALVWGILPPADAQSVDEGFATGGAAAA
                     WELWQPAMQAILSRNTAEAEKLFTELLELNPSPFRIALLADRTMNRTTLGGAILLLEQ
                     DLEAKALEASGQRVAEMLAEGREQMAQADDGWYFSALGRFDVAKANFQALLDSKPDAV
                     ALLEFTDQVAQRRDVLLRLTDNEIVGEAVRGVLKTLEHGEELIKADPVRIKHRIDQLA
                     GPPRAFQNSVALLAQSGEWAIPFMVEYLRDREKQALTQPILRALPQIGRAGINPLVYA
                     IRVDNPVVQTYLVRILGQVGYAQALPYLLQLREKPNTPPEVLAAVDQALADLAAHGAA
                     VPPGMTAAMAFYWLANEYYADAGSLAADPRLDNANVWFWRDGLVQNLPVPTQIFNEVM
                     CMRCCEEALHLDPAMEDALALWLAANFRRVAQLGDTGTDTTVPSDFPSPLYFAQTAGT
                     SACLKALSRALDDKDPAVALGIIEALRNVAGPASVTADAAGRLPLAEALLFPDQVVRI
                     KAALTLGAARPVQPFLNYQNLMPVFAEALLLHNGSRRAAVLDPDVESANAFSGMLRDA
                     GYEVTSDTDFHAGLAKVRQHVPALDVVVLASNIGTPGLLDALKHLRSEFRFSHTPVVL
                     VTKPGDADTVRDLVRADHRLAEVRPGATQDALRSAIDRVSRAVGMIQITPEAGTAIAL
                     DAAGVLETLAVTGNPLFDPAQLEQPLINALGSSDAALRSTTARVLSHVCSTAAQTALA
                     KIALDAGREAELRIEMFDVLAQAGKQCGNLLGQPQVQEIIKLAENEADMTIRTAASQA
                     LGALNVPVGSGSQIIRNLYRE"
     gene            7251..7898
                     /locus_tag="PLU99_08150"
     CDS             7251..7898
                     /locus_tag="PLU99_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17067.1"
                     /translation="MPLILGTIALLAFQPAAAPVGKGPQPSPMPVSWELDLRFLDPQR
                     IEVQVPGASAPEVYWYMVYTVTNHTGRTQRFFPTFQLVTDDLHVVDTDMGIHALVFEA
                     IRERHRRTHPDLVHPTKAIGDLLTGDDNALESVAIWRGVDSDANRIAIYVAGLCGEAR
                     VVRNPTFDSDRPEGPENPRQFTLRKTLEICYNLRGDAAARTLVEPERTSIRWLMR"
     gene            7999..8247
                     /gene="rpmA"
                     /locus_tag="PLU99_08155"
     CDS             7999..8247
                     /gene="rpmA"
                     /locus_tag="PLU99_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOO17068.1"
                     /translation="MAHKKGQGSSRNGRDSNAQYRGVKVYGGQTVRTGAIIVRQCGTP
                     FKAGLNVGRGRDDTLFALADGQVEYRGRFVSVVPVAAG"
     gene            8573..9112
                     /gene="rnhA"
                     /locus_tag="PLU99_08160"
     CDS             8573..9112
                     /gene="rnhA"
                     /locus_tag="PLU99_08160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOO17069.1"
                     /translation="MAEAVDVILFTDGACSGNPGPGGWACILRHCATGYEKRLSGGEP
                     LTTNNRMELMAVIQGLAALKRAPLRVRVVTDSQYVSRGMTEWVPRWQAAGWRRKRPGK
                     ARALPVKNLELWKRLVELCSQHKVEFQFVAGHAGHPENEECDRLAVAAAKEAAKLPRP
                     SALDSDGSNDEPVLPFNSE"
     gene            9344..9673
                     /locus_tag="PLU99_08165"
     CDS             9344..9673
                     /locus_tag="PLU99_08165"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOO17070.1"
                     /translation="MTDLAQRQCVPCRGGVPPLRGAELRSLLDQLDGWEAPKEHHLHK
                     DFSFPDFMTALAFVNRVGELAESEGHHPDIFLSWGKVGIDIWTHKIDGLTESDFVLAA
                     KIDNLPR"
     gene            9955..10902
                     /locus_tag="PLU99_08170"
     CDS             9955..10902
                     /locus_tag="PLU99_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17071.1"
                     /translation="MPRATLPSDVDRDFPAGARILTGFDPRTPGTGWQPGDRVLYAVR
                     LEKGGRSQTWYALIKVIRANLQPGMRLTLDADDDRGDPASARTRLVGHRTRTLTAPRL
                     TGRGAQWARERGLQLSPTSVLVRIDVFDEHARPIADVHQFIFSDYLQAGLYQSCDLVR
                     RHYFDDWDPNDSDIRQVSLSFVSLFSLGELLWATPHVGQIFNEIVRLPSWISILLNLG
                     VRPIFTIHYEQARRESRALPALADGRKAYRVPFEITLNDELALRCYVTAAPNEPPLQL
                     CGGIVALEGADVRDPQHRFTVRLLAARQSRPEIQTASRD"
     gene            complement(11102..11251)
                     /locus_tag="PLU99_08175"
     CDS             complement(11102..11251)
                     /locus_tag="PLU99_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17072.1"
                     /translation="MPITGAKLPAFSSRLSSLTCVDAAAFDVDADDDIDLADLALFQQ
                     LYQGG"
BASE COUNT         2049 a         3855 c         3573 g         1995 t
ORIGIN      
        1 gcgaaaagcg atgacagagg aagcgggcat ttcggagata gcgctgccac cagtgccctg
       61 acccggggca ttggcctcgc gcttctccgc tgcgcgacgt tgatctgtac cgcggacgct
      121 acgccaccac cggcaaaagc agccgcgacg cgtgcgcgcg atcgtggtag acgcgattcg
      181 tcgcgataac cgtttcaacc gcgctgcccg gcggctcgag cgtgttcgta tgcgcggcga
      241 acttcggaac cgcggctgag gcgacttgca accgcaaccg gtgtcctttc aggatgcgga
      301 tgcccgtggc ccacaggtcg atgtggacct gaacgacttc gcccggcggg accggttcac
      361 gcttctccag cgaattccgc agagccagcc gcacattgcc gtcgcagagc tgacgcgcca
      421 ggccgttttc gtgcacgtcg cacagcatag cgcaaaactc ggtatccgcg gccgtgctcg
      481 aaacccacaa ttcggcgaac acccgcccgc agatgaccag gtcctcgctc agcggctcag
      541 tcgtgtaaca cacgacgtcg tcgcgacgct gcacgggccg ggcatcgaag ggcccgccga
      601 cctgaagcgt tccagcgtcg tacagatatg gcgtgggatg ctgcgggtca tatgtgtact
      661 catcaaacgc agagccggac gtcatttccc gcgccagcag tccatcgcca aacagcgaat
      721 tcgcccggcc tgaagaattc aggtaatacg ccacttcctg cgtcccgggc ggcggccaat
      781 catcgaaatc gtgccaatca ttagagccca tcaggaaaat gcgcgcccgc ggccgatccc
      841 cccaactctc aggccgcccg ccgatctgct tgtccagcca atcgagaatg aacccattga
      901 tgtcaatgac ttcctgcggc ccgaaatcta cgccctgcac caccgtcttc gtgttcgtcg
      961 catgcggcca gggtccgatc agcacgtact gctccgcccc gctttttgcc aggcgccgcg
     1021 cattcggaaa gttgttgtac gtgctcgccc catcgtcgtc ataccagccg gtgatgtgca
     1081 gcgccggcag atcgagctcc ggcatgcggt gcatgtaaca cgcccgctgc caatattcat
     1141 ccttcagcgg gtgcttcatc cactcctgcc acgccgcgct tgaaaagccc aaatgctcat
     1201 ccagccggtt cagcggccgg aagttgtaaa ccgattccca gttgaactgc cccacgtcct
     1261 gattcgtccg gccgtgcacc agcagcgccc aggtgatcat gctccagcac accgagccgt
     1321 tccaccaggg gacgttgatg accgggtcgg gcagcgccac gacgggaatg atggccttga
     1381 ccgccggatg gtgcccgaat gcccccatcc acacgcagaa gccttcatat gagcggccat
     1441 agagcgcgat cttgccgttg caaaactcct gttccgccag ccaggcctgc gcggcatgcc
     1501 cgtcggcggc ctcgttgaag aacggctccc attcgccctc gctatcgtag cggccgcgca
     1561 cgtcctgtat ggccaccgcg tagccgttgc tggcgaactc gcgcgccagc ggaaccttct
     1621 tgaccgggtc gttgttgttg tacggcgtgc gaatgaggac gcacggccac ggcccgcggc
     1681 cgtccggcag atagacgtca gtggacagcc gcacgccgtc ggcggcaggg cagcccaggt
     1741 tcagacgcac tttcacgcca tagcgaggac gagtcggctc atgcatcggt cacctcactt
     1801 ctgcgaatcg tcacggcaga tcgcgttatc cgcagcacaa tagtcgagtc cgcagcaggg
     1861 cttcaagcag gctcccggtg tgccactgca tgaagcggca gcttcaagca gtgctcatcg
     1921 cggttccggg tgctcatcgc ggctccgggt cagtcacccc gtgcccgccc gggaaatcca
     1981 cccgccgctg agcacgatgt cgccatcgta aaacacgacc gcttgcccgg gcgtcaccgc
     2041 ggattgcggt tcatcgaagc gcgcctcgac ctcgccgcca ggaaggacgg tgatgaaccc
     2101 ggcggccggc ttgtgcatgt aacgaacctt tatgtccgcg gggcgaggct ggccgaccgc
     2161 aggggggtcg gtcaaccagt tcaccttctc cgccaccagc ccgccgctca gcaggtcctc
     2221 gcgcgacccg agcgtgacga cgttgttcaa aacgtcgagc ttcgtgacgt aggccggccg
     2281 gcccacggca atgcccagcc cttgccgctg gccgatggtg tagttggcga tgcccgagtg
     2341 cgttccgagc acgcggccgg ccgggtcgcg cacctccccg acgcgctgcg tgtcggggcg
     2401 gcgctgccgc acaagctcct tgtagtcgcc ccctggcacg aagcagattt cctggctgtc
     2461 gggcttgtcg tggacgcgca gccccagctc ggccgcgatg ctccgcacct gggccttgtc
     2521 gggcatgtcg ccgagcggga acaggcagcg ccccagtgcg gcccggtcga tgccgaaaag
     2581 cacgtacgac tggtccttgg cgaggttgga ggcgcgggcc aataggattt gcccgtcgcg
     2641 gcgtaggatg cgggcataat ggccggtcgc gacgaactcg acgtccagcg cgttcgcgta
     2701 gcgcagcagt ttgccgaatt tcagatgcgt gttgcacacc acgcacgggt tgggcgtgcg
     2761 gccgcgggcg tactcgtcca cgaaataatc gatgatctgg tggaaatcct gctcgaagtt
     2821 cagggcgtaa aacggtatgc ccagtcggcc ggcaatggcg cgggcgtcct gcgcgtcagc
     2881 cgctgagcag caaccctgcc ccagacggcg gcgctgggcg ggcgaggagt ttggcaaggt
     2941 gcctccgggc aaggtcgagg cgcctacggg cgagtttccc acggacggcg aggcgcctgg
     3001 agagggcgag gcgcctggcg aggccacccc ggggaaagtc gacgcgctcg agggcgaggt
     3061 ttctgccgag tcgcggcacg cgtccgcagc ttcgccgagg gcgcattcag tcggctcggg
     3121 cgcctgtgcg ccgttgcgca taaagacgcc gatgcactcg aagccctgct gcttcaggag
     3181 ccaggcggac acggcggagt ccacgccgcc cgacatcgcc acgataacct tgccgttgcg
     3241 cgacattacc gctttctagt cgcccgccgg ccaaaagcaa gccgccggcg gcgacccggc
     3301 gctctcagcc aaggcaaccg ccaagacgcc gagagccgtg gcggcgcggc gccgatcacg
     3361 cgcttggcgg agtccatatt gaactgcgcg gttccggcct gaacctcttg caggcgctcg
     3421 catagacgta cgattagaaa ggaatcgtca gcagtcaccc gggcgtgagc aatgtgcggc
     3481 cagaggtctt cgtgcaccgc gacaacaaga attcggtcgc ggatcgatac gatcgtgccg
     3541 ctcgtgtcct tctggccgca acgcaactcg cacgggtgtg tcgtcacgga ggtgaatctc
     3601 gtcggcaagc tgaaactggt agatgtagtt tccgtcgctg accccaataa atctcccgcc
     3661 aactaggtca aagctggtcg atccgcccga cttctggatc gcggcgacct ccaatgagag
     3721 cgctgcgatc atatttgcca agattccgct atcgtgtgtc acaactccgt tcctccggcg
     3781 cgaatctcgt cccgacagcc cgcccgaatc gctatttgaa cccgcctgtg ttgccacggc
     3841 ccagatcgtc tcaggttcag gtcggtacgc gcgtcagcat gcaaattgcc gtcgtgaccc
     3901 agcaacagaa ttcaccgcca tggtcacgtc ctgagcaaga gaactaccag cgcgcccgtt
     3961 ggtagcgagt aagcaaggtc caattcggcc ttgtactcgc tgcaggtgaa atcaaaaaaa
     4021 ccgtccatgg ccgcatgcca tcctatcgcc gagtatttgg cagcatccgg cgcggctggg
     4081 gttgatgcaa ggacgcgatt ggtggtgtgc gatgaataga ccccatccac aatcctcgcc
     4141 tgcgatctga cacgatggcg ggcaggagca cagtacgtcg accgctgaga ttcgcgcatc
     4201 agcaaacgaa ggtgcgagac gaattcgtca acgcttggtc gctcatgcgg tcgctcatgc
     4261 gtgacaagaa agcagcctga tgggcttcat gtccggcact agagcttaat gctcgctagg
     4321 cgcgatgaga gagcagtccg ccatggggct ttttttatca gacgaatatc aagcgaatac
     4381 cgtacgactc gcccgggccc cccatcggcg tccgttgaac gagcggcatt ggcggtcgga
     4441 tgcatgacgt ttcggggcgg caccgcatga tcgaagcggg catcgcgcgg atcggagacc
     4501 cttggcggca ttccgcggcg gcacgccccg gctctgctct ggcgttgcgg cacccgagtt
     4561 cgaggctcgc gtccccaagc tcggcacccg aggttcaaac tcgaaacccc ggctcgaaac
     4621 ccaagctcaa aacccgaaac tcaatgccga aacccgaaac tcaatgttga aaccgcgaat
     4681 ccgaaattga caccccttcc gtcaacccta ataatttctc gctattagac caaccggctg
     4741 cgcgcaacct tacgaccagg cttgcggcgc gtggaggtaa atatgtccaa atgtccccgg
     4801 ctccttcgtc ttgccggcat cgccgccggt ttcgctcttg tttggggaat tttgcccccc
     4861 gccgacgccc agagcgttga tgaaggcttc gccacgggcg gcgcggctgc ggcctgggag
     4921 ctgtggcagc cggcgatgca ggccatcctc agccgcaaca ccgccgaagc ggagaagctg
     4981 ttcaccgagt tgctcgaact caatccgtcg cctttccgca tcgcgctctt ggccgaccgg
     5041 accatgaacc gcaccaccct gggcggcgct atcctcttgc tggagcagga cttggaggcg
     5101 aaagccctgg aagccagcgg gcagcgcgta gccgaaatgc tggccgaagg ccgcgagcag
     5161 atggctcagg cggacgacgg ctggtacttc tcggccctgg ggcggttcga cgtggccaag
     5221 gccaatttcc aggccctgct ggacagcaag cccgacgccg tggccttgct ggagttcacc
     5281 gaccaggtag cccagcgccg cgacgtcctc ctgcgcctca ctgacaacga gatcgtcgga
     5341 gaggcggtgc gcggggtcct gaagacgctg gaacacggcg aagagctgat caaggccgac
     5401 ccggtccgca tcaagcaccg catcgaccag cttgccggcc cgccgcgggc gtttcaaaac
     5461 tcggtcgcct tgctggcgca gtcgggcgag tgggccatcc ccttcatggt cgagtacctg
     5521 cgagaccgcg agaaacaggc gctcacgcaa ccgatcctgc gcgctttgcc gcagatcggc
     5581 cgcgcgggca tcaatcccct ggtctacgcc atccgggtcg ataacccggt agttcagacc
     5641 tatttggtgc ggattctggg gcaggtcggc tacgcccagg cgctgcccta cctgctgcaa
     5701 ctgagggaaa agcccaacac cccgccggaa gtcttggcgg cggtcgatca ggctttggct
     5761 gatctcgcag cccacggcgc ggcagtgcct ccgggcatga cggccgcgat ggctttctac
     5821 tggctggcga atgaatacta cgcggacgcc ggttcgctgg cggccgatcc gcgcctggat
     5881 aacgccaacg tgtggttctg gcgcgacggc ctggttcaga atcttccggt gccgacgcaa
     5941 atcttcaacg aagtaatgtg catgcggtgc tgcgaggaag ctctgcacct cgacccggct
     6001 atggaggacg cgctcgcgct atggctggcc gccaatttcc ggcgcgtcgc gcaactgggc
     6061 gacaccggca ccgataccac cgtgcccagt gatttcccaa gcccgctgta ctttgctcag
     6121 acggccggca ccagcgcttg tctgaaagcg ctcagccggg ccctggacga caaagatccg
     6181 gccgtggcgc tgggcataat cgaggcattg cgcaacgtgg ccggtcccgc cagtgtgacg
     6241 gccgacgccg ccggccggct gccgctggcg gaggcgctgc tgtttccgga tcaggtggtt
     6301 cgcatcaagg ccgcgttgac gctaggcgct gcccggccgg tgcaaccgtt cctgaactac
     6361 cagaacctga tgccggtgtt tgctgaggcg ttgctgttgc acaacggcag ccggcgcgcg
     6421 gcggtgctgg atccggacgt ggaaagcgcg aacgcctttt caggcatgct tcgcgacgcc
     6481 ggctatgagg tcaccagcga caccgacttt cacgccggcc tcgcgaaggt gcgccagcac
     6541 gtgccggcgc tcgacgtcgt cgttttggcc tccaacatcg gcacgccggg gcttctcgat
     6601 gctttgaaac atttgcgcag cgaattccgc ttttcccata cgccggtcgt tctggttacc
     6661 aagccgggtg acgcggacac ggtccgtgac ctcgtacggg cggaccatcg cctggctgag
     6721 gtacgtccgg gcgccactca ggatgctttg cggtcggcga tcgatcgcgt cagccgggcc
     6781 gtcggcatga ttcagatcac gccggaggcc ggcacggcga tcgcgctgga tgcggcgggc
     6841 gtcctggaga ctctcgcggt cacagggaat cctttgttcg atccggcgca gctcgagcag
     6901 ccgttgatca acgccctggg cagcagtgat gccgccttgc gttcgaccac ggcgcgtgtg
     6961 ctgtcgcacg tttgcagcac cgcggcccaa acggcgctgg cgaagatcgc tctcgatgcg
     7021 ggacgcgagg cggagctgcg aatcgaaatg ttcgatgtgt tggcgcaggc gggcaagcaa
     7081 tgcggcaacc tgctgggtca gccgcaggtg caggaaatca tcaagttggc ggagaacgaa
     7141 gctgacatga cgattcggac cgcggcgtcg caagcgttgg gcgcgctgaa cgtaccggtg
     7201 ggcagcggca gtcagatcat ccgcaacttg tatcgggagt agcagtctcc atgccgctga
     7261 ttctgggaac tattgcgttg ctggcgtttc aacccgctgc ggccccggta gggaaggggc
     7321 cccagccgtc gccgatgccg gtctcttggg agctggattt gcggtttctc gacccgcagc
     7381 ggatcgaggt gcaggtcccc ggggcgtcgg cgccggaagt gtactggtac atggtttaca
     7441 cggtgacgaa ccacaccggg cgaacgcagc ggtttttccc cacgtttcag ctcgtgactg
     7501 acgacctgca cgtggtggat accgacatgg gcatccacgc cctggtattc gaggccatcc
     7561 gggaacggca ccgccgaacg catccggacc tggtgcaccc gaccaaggcc atcggcgacc
     7621 tgctgaccgg ggacgataac gccctggagt cggtggccat ctggcgcggc gttgattccg
     7681 acgccaaccg catcgccatc tacgtagcgg ggctgtgcgg agaggcccgg gtggtgcgga
     7741 acccgacgtt cgactcggac cgtccggagg ggccggaaaa tccgcgccag ttcaccttac
     7801 gcaagacgct ggaaatctgt tataatctgc ggggtgacgc cgccgcccgg accctggtgg
     7861 agcccgagcg gacgagtatt cgttggttaa tgcggtaatc gtgaggtaac cgagccccgg
     7921 agttgcactg cgggggatgg gttttcgtca gggactcgcg gcacgacagc cgcaacgtgt
     7981 ttttgaacgg aattcgttat ggcacataag aagggacaag gttcgtcgcg gaacgggcgt
     8041 gacagcaatg cgcagtatcg cggcgtgaag gtttatggcg gacagacggt gcggacgggc
     8101 gcgatcatcg tgcgtcagtg cggcacgccc ttcaaggcgg ggctgaacgt gggccgcggg
     8161 cgagacgaca cattgttcgc cctggccgac gggcaggtcg agtaccgcgg gcggttcgtg
     8221 tcggtggtgc cggtagccgc cggctagccg aaccctgcga aaacggtcta ttagcgcagc
     8281 cccggacaac tggtgctgcg cttttctttt gagcagagcc ggggcgtgcc gccccggaat
     8341 gatgccaacc cgccccaatt cgcgtttgtg cgagcgaaac tgaggcgtgc cgccccggac
     8401 tgatgcaggg cactcgcatt tcgcggagag cgaaactgat gcgtgtcgcc ccggaatgac
     8461 ttaagtgctc gccctttcgg cggaaagccc ttcactcatt cccgccgcct ggcagagcca
     8521 tgccctcatc gataaactcg cccgccctcg ccccgatgga gcgcgatccg acgtggctga
     8581 agcggtcgat gtcatcctgt tcaccgacgg cgcctgttcc ggcaaccccg gcccgggcgg
     8641 ctgggcctgc attttgcgcc attgcgcgac cggctacgaa aagcggctct ccggcggcga
     8701 gccgctaacg accaacaatc gcatggaact catggcggtg atccaaggtc tcgccgccct
     8761 caagcgtgcg ccgctgcgcg tgcgagtagt aaccgacagc caatacgttt cgcgcggcat
     8821 gaccgagtgg gtaccgcgct ggcaagccgc cggctggcgg cgcaaacgcc ccggcaaggc
     8881 ccgcgcccta cccgtaaaga acctcgaact gtggaaacgg ctggtcgagc tctgctcgca
     8941 gcacaaggtc gagtttcaat tcgtcgccgg gcacgccggc catccggaga atgaagaatg
     9001 cgatcggctg gcggtcgcag cggcgaagga agccgcgaag ctgccgcgcc cgagcgctct
     9061 cgactccgat ggctccaacg acgaaccagt gctgccgttt aattccgagt agtgactcga
     9121 ctgtaagaca cgcagtgagg cgcacgccgc cgccattacg catttctaat tccgggtcgg
     9181 agcgcttgct gaagcagaac gcccgaatcg ccccttcgta agcttcagct tcgcacccct
     9241 cgtcttcctc cacgctcggc gacatttccg cccgccttct cgcgcgctta agccgcatag
     9301 aatccgctgc acccgcgcac gcacacccga cgggagcgac acaatgacgg acttagcgca
     9361 gagacagtgc gtaccctgcc gcggcggcgt cccacccctg cgcggcgccg aactcaggtc
     9421 cctgctcgac cagcttgacg gctgggaagc ccccaaagag caccacctgc acaaagactt
     9481 ctcgttccct gacttcatga ccgcgctcgc cttcgtcaac cgcgtcggcg aattggccga
     9541 atccgaaggg caccaccccg acatctttct gtcctgggga aaggtgggga tcgatatatg
     9601 gacgcacaag atcgacgggc tgaccgaaag cgacttcgtc ctcgccgcca aaatcgacaa
     9661 cctgccgcga tgagcacggc ccacgccgct ctcgcctggc cggtgcgccg cctcgccctt
     9721 tgtttggccg cgctgctgac gctggtcacg ctccccggct gcgtgcgcca ctgcccgcac
     9781 gccgctgcgt ccccgcggcc tgatgctcca gagcgcactg tcgcggtcag cgggccttcg
     9841 tccggccaga ggcttgccga cgcggaccgg atgctttcta cagcggaccg cgcgcccggc
     9901 agcgtgaacg gggcgcctgg cgcgccggca gcgttggtcg cgcacaagga gcgcgtgccc
     9961 cgggccacac tcccgtcaga cgtcgaccgt gacttccccg ccggcgcacg cattttgacc
    10021 ggcttcgatc cgcgcacgcc cggcaccggc tggcagcccg gcgaccgcgt cctttacgcg
    10081 gttcgcctgg aaaaaggcgg gcgctcgcag acttggtacg ccttgatcaa ggtcatccgc
    10141 gccaacctcc aacccggcat gcgtctgacc ttggacgccg acgacgaccg cggcgatccg
    10201 gccagcgctc gcacacgcct ggtcggccac cgcacccgca cgctcaccgc tccgcgcctg
    10261 accggccgcg gcgcgcagtg ggctcgcgag cgcggcctgc aactttcgcc caccagcgtc
    10321 ctggtgcgca tcgacgtatt cgacgagcac gcccggccca tcgccgacgt gcaccagttc
    10381 atcttcagcg actatctcca agccggcctg taccagagct gtgacctggt ccggcgccat
    10441 tacttcgatg attgggaccc caacgacagc gacatccgcc aggtcagcct gagcttcgtc
    10501 tccctctttt ccctgggaga actgctctgg gccaccccgc acgtcggcca gatcttcaac
    10561 gaaatcgtgc gcctgccgtc ctggatctcg attctgctca atctgggcgt gcggccaatc
    10621 ttcaccatcc actacgagca ggcccggcgc gaatcccgcg ccttgccggc gctggccgac
    10681 gggcgtaagg cgtaccgcgt gccctttgaa ataacgctga atgacgaact cgctttgcgc
    10741 tgctacgtca cggccgcacc aaacgagccg cccctgcaac tatgcggcgg catcgtcgcg
    10801 ctcgagggcg ccgacgtgcg cgacccgcag caccgcttta ccgttcgcct cctggccgcg
    10861 cgccagagcc ggccggaaat tcaaaccgcc agccgcgatt gaaggtcccg ccgcgccggc
    10921 ccgaggtcga gcgggtgcca tacccaagcg ctaaagcgcg ggtgccatgc ccaagcgcca
    10981 agcgcgggca tgccctcggc tacctgctcg gcgtcgcgta accggaattc gccatttgcc
    11041 aggatatcag tgaagcccaa gctcgcccct gggccgcctg cgccgccggc gcgacagtgg
    11101 ttcaaccgcc ctgatagagc tgctggaaca gggccagatc agccagatca atgtcgtcgt
    11161 cggcatccac gtcgaacgcc gccgcgtcaa cgcacgtcag gctggatagc cgagagctga
    11221 aagctggcag cttcgcccca gttatcggca cattatcaaa accgggtgcg catttcgatg
    11281 agcacctgcg cacctgcccc gggcgctatc ccgccccacg ttgttatgag acgcgtgatc
    11341 acctgcttgg ccacctgacc cctgaacccc cccccccggc tctgctcttc gttcgtcttg
    11401 atccctcgat cccttgatcc ctcgatccct ctctcggcag tattccgggg cgacacgccc
    11461 cgccgctgcg gt
//
LOCUS       DAOSYS010000107        11460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4809_length_11460_cov_4.585007, whole genome shotgun sequence.
ACCESSION   DAOSYS010000107 DAOSYS010000000
VERSION     DAOSYS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_11460_cov_4.585007"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLU99_08180"
     CDS             complement(<1..927)
                     /locus_tag="PLU99_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO17073.1"
                     /translation="MIQMQTAPRAAEAAKPSRNMRLIQVTPDVACIQNVMVNLYFVGH
                     NRPGADWVLIDAGLPYSAQGILRAAERHFGAGARPRAIYLTHGHFDHVGGLRELAEFW
                     DVPVFAHELELPYLTGLSSYAPPDPNVGGGLLAWISSLYPRGPIDLGRRVNALQADDL
                     PYLDKWRWIHTPGHTPGHVSFFREQDRLLIAGDAFTTTKPESLRAQLRPQSGVYGPPA
                     YFTSDWASAWSSVQRLASLHPAVAATGHGLPMKGAELEQDLQTLARDFDRLAVPRRGR
                     YVLQPAVADKTGVVYLPPAPVNALFVGALGLAG"
     gene            1254..4739
                     /gene="mfd"
                     /locus_tag="PLU99_08185"
     CDS             1254..4739
                     /gene="mfd"
                     /locus_tag="PLU99_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOO17074.1"
                     /translation="MNLFAAVHGDPRFEELLRLLATERGVAAAEGLWGSAAPVLAALA
                     AQRLGRPLLLITAHADEADDCRDDIETAVGRAPDLLPQLEQIAGESTEADELAAERLR
                     LCMEVMRWESGRVITAPEREAARPESGASPQKVVPEEAPGASPPYHPITESRCHDHSG
                     PPAIVAPIMALMQTVPSPEAIAATSLALDVGLTLEPAELARWLSEHGFSRCDAVDVPG
                     DFAQRGGIVDVFSNAHTDPVRIEFFGDQIESIRLFDLGTQRSSQLLQSVQIPAMSLPR
                     LRRADPRRGLSTSFLAFLPADTLIALAEPAEVQELGRTYWQRLGEPAGMVPVEAVFRR
                     AADFSQLHLQRFKGGGPRTVDFAVRSLPQFEPKSTEALQALVELARECETYVLCDNPA
                     EEQRFIELLDQLPERSANLHTAVGVLHRGFQWGHAAEGGQTTPAQQTAYVPHHELFHR
                     YRQRRTLRRVAPARPIDSFFDLDTGDLVLHTLHGIGRFLGLKTLDRAGRPEEYLAIEF
                     AGKAVVHVATSQIHLVQKYIGAVRGKPKLSTLGGSAWKNTKARVAEAVTDLAGEMLAI
                     QAERETQPGIAYPQDTAWQREFEESFLFEETEDQLRALEDIKRDMLRPRPMDRLLCGD
                     VGYGKTELAIRAAFKVVEYGKQVAVLVPTTVLAEQHHQTFRERLADYPFSIEVLSRFR
                     TKREQDRIVEAARKGRVDILIGTHRLLSPDVRFADLGLVVIDEEQRFGVDAKERLKRL
                     RVTVDVLTLSATPIPRTLHMAMLGIRDISSLATPPLDRRAIVTQVRLWDDGLIREAVL
                     RELNRDGQVYFVHNFVRDIHAVANRLRSIVPEARVVIGHGQMSAHELEDVMLKFLRRE
                     ADVLVSTNIIESGLDIPSANTIFVNRAERFGLADLHQLRGRVGRSKNRAYAYMLLSPQ
                     HPVKDTAAKRLKAIEHYSELGAGFQIAMRDLEIRGAGNLLGAEQSGHIEAVGYEMYCQ
                     MLEQAVRRMKNEPAEPFKPVNLELGVTASIPRAYIRSDRQRMEVYKRSTHCRTPEDLA
                     VLHDDLRDAFGPLPEEVQTLLKLAELRVLAQRWGIRSIVLDPPDVIFAVEDFQKARPL
                     FETGPGSPRVPDPQTVHWRLPKRFLEMPTLLTILRRQLAGEAKEAIPQGDPNEPPPSA
                     PPVSRGRKRAGARRL"
     gene            4849..6945
                     /locus_tag="PLU99_08190"
     CDS             4849..6945
                     /locus_tag="PLU99_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOO17075.1"
                     /translation="MLSMRLRGMVLAAALAGAYFGGTSFADTLEFADGRKLENCYVRD
                     EGTRILVWRQLADVGQPPEAFPRSAVKSYQIERGPEWDRKPDLPDLSVTFIEVDPKNY
                     SLHGRIQVDQFGRPLPTGAPVLKSQGIRDYMEPNKIIAGVRTNYRPGEELTLTAHVKN
                     VGFAPAGPFRYTCLVDDEPILEGRFTDALNENQEVTFAAKWKWRTGQYTVRFRIEPEH
                     PEIATINNETIAPLWGWGLIYIVNPGRVAAWREYRNAYGTFSWEDYYRWHIDLMNQLF
                     ADSVYPAAPEGIKARVRLDRIVYATDVPAAEQARWKENGLADDQGARVWHDSPEELAS
                     GKWAPPEHAARNQTEWTLLHDLGHELGLPDYQTLDYAGAEPHVAPDTGDKIGHSARYP
                     TQMMYRPGPWLFGEADAGYLNFSIDKPRGYRGDMFFALPRILAVRLTDMSGRGVPGAF
                     VEFFQRGVVVEHEKQAGGIEDLKYWSVVEDGNFDHPVSAYPVMTGRSDPGGVVRLIPR
                     PAESIRTLNGFQRRLNGFGNIDPEGKRGLMLVRVTKDDRQAHFWLDITEAITAWFRGE
                     QEYYEFALQTPFGSLGAPPAPRDVKAERKGGHEVRITWAPPSAEREQQYLQHVVGYRV
                     YGRVGNGGLNDQPWFPVAVSPPEGLSVTVDMRQFPVKFEHGSVVRLGVASAGECSNVS
                     ELVEVVLPVATAAAGE"
     gene            7147..9069
                     /locus_tag="PLU99_08195"
     CDS             7147..9069
                     /locus_tag="PLU99_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO17076.1"
                     /translation="MRILSVWRWTLVAVFVCVPVLAGAGSPQVVDVRVLENSPERITV
                     SYSLGDYELRNLTIDGQDWSVPRLPGEAIMLEKGAPSLPKVCRSVIIPDDAAMSVAVV
                     AADYYDVACEVAPSKGSLKRNVNPASVPYEFGPAYSQNRFFPGEVVKLGQPYLLRELR
                     GVCIELYPFQYNPGSGILRVYTDVTVELTPAGPGKVNVKHAGRDFKKPTRAFDSIHSA
                     HFLNFGPRLRYAPLDEQGDLLIICHDAWIPYVQPLAAHKNRIGINTTVVGVSSIGLNP
                     DAIKSYIQNVYDTSDLAFVLLVGDSAQVPTPIAYGGSSDATYAKLAGDDNYPDIVVGR
                     FSAESAAHVQTQVLRTIEYETMPATRQPWFKRAVGIASSQGDGDGDEGQADFVHMGEI
                     RDWLLDYDYTEVDEIYDPGASAGAVTAAVNAGRGIINYCGHGTRSAWTTTRFSSTHIA
                     ELTNYNQLPFIFSVACVNGQFANGTCFAETWLRATRDGEPTGAVAAYMSSINQDWAPP
                     MEAQDEFNLLLTREDYFSFGALCYAGSCAMMDSYGSTPGSDGLVNFDTWILFGDPSLR
                     VFGYVQPRHPAGDLNCDGAVNAFDIDPFVLALTDPAGYAAAYAECDINNADINNDGTV
                     NAFDIDPFVAVLTGGG"
     assembly_gap    9601..9700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9979..>11460)
                     /locus_tag="PLU99_08200"
     CDS             complement(9979..>11460)
                     /locus_tag="PLU99_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HOO17077.1"
                     /translation="RLRERTWAPQDQPMFRARNIHYELSDRLRGLAPGGIGAMHHLAR
                     HAGLIAAIDRRLHLLKIHLPYHESDHVLNVAYNILAGGSCLEDLELWRNDEVYLDALG
                     AQRIPDPTTEGDFCRRFTAHDVEILQDTLNEARLGVWRQQPKEFFKLAILDADGVVAE
                     TYGECKQGIGRAYNGVWGYHPLVVSLANTQEPLYLANRSGNRPSNEGAAGYLDRAIAL
                     CRRAGFKRVLLRGDTDFSQTEHLDRWHLDGVQFLFGLDARANLVELAENLPESAWKTL
                     QRPPKYEVSTKPRQRPTNVKEEIVVANGYRNIKLQGEAVACFAYKPYACKRQYTVVAL
                     RKDLAVEHGQCRFFEDVRYFFYLTNLPAGDPAELVYLANDRCNQENLLEQLRNGVRAL
                     NMPVDGLVSNWAYMVMASVAWSLKAWFALRLPERGRWKEKHRQQKRQVLTMEFKRFVN
                     SFVRVPCQIVRAGRRIVYRLLAWNPWQAVFARGLVALGCPLRC"
BASE COUNT         2037 a         3589 c         3707 g         2027 t
ORIGIN      
        1 cccggccaat ccgagcgcgc ccacaaacag cgcgttgacc ggggccggag gaaggtaaac
       61 gacccccgtc ttgtcggcga cggccggctg tagcacgtag cgcccgcggc gcggaaccgc
      121 caggcggtca aagtcgcgcg ccagcgtttg aagatcttgc tccaattccg cgcctttcat
      181 cggcagacca tggcccgtcg cggccacagc cgggtgcagg ctggccaggc gttgtacgga
      241 tgaccaggcc gaggcccagt cgctggtgaa gtaggccggt gggccgtaaa cgcccgactg
      301 cggccggagc tgcgcgcgca acgattccgg cttggtggtc gtgaacgcgt ccccggcgat
      361 caagaggcgg tcttgctcgc ggaagaacga aacatgcccc ggcgtatgac ccggagtgtg
      421 aatccagcgc catttgtcca ggtacggcag atcgtccgct tgcaaggcgt tcacgcggcg
      481 cccgagatcg atcggcccac gcgggtacaa actggagatc caagccagca acccgccgcc
      541 cacattggga tccggcggag cgtacgagga caacccggtc aggtacggca attccagctc
      601 atgcgcaaaa accggaacat cccaaaattc ggccagttcg cggaggccgc cgacgtggtc
      661 gaagtgcccg tgcgtcaggt aaatggcccg cgggcgcgcc cccgcgccaa agtgcctttc
      721 ggccgcccgc agaatgcctt gggccgagta cggcaagccg gcatcgatca gcacccagtc
      781 cgcaccgggg cggttatggc cgacgaagta caggttaacc ataacgttct gaatgcaagc
      841 gacgtccggc gtcacctgaa tgaggcgcat gtttcgggac ggcttcgccg cctcggcggc
      901 acggggggcg gtctgcatct ggatcatctg gatacctcat ccgttgccct tggcacagag
      961 attatgccgg taacggattc cggcggcgtt aaaaacggct tcatttgccg gacgtccagg
     1021 cggccctggg gtacccacgt atggcacgcg gcgccggtgt gtgtcgtggt ttcgcgtgcc
     1081 gccgggtgcc atgctcaagc cgcgtagcgg cgcgggtatg cccttgccgg tagcctccca
     1141 tctgccccgt cgctgacgaa ggcatgcccg cgcttacagc ttgggcatgg cgcccatggc
     1201 acccgactgg taaggggagg cgacgtaatg atttgccgat aaaattacga gcagtgaatt
     1261 tgtttgccgc cgtgcatggc gacccgcgtt tcgaggagct gctccggctg ctggcgacgg
     1321 aaaggggggt ggctgcggcc gagggcttgt gggggtccgc tgcgcctgtt ttggctgcac
     1381 tcgccgctca gcggctgggc cggccgctac tcctcatcac cgcccacgcc gacgaggccg
     1441 acgactgccg cgacgatatc gagacggccg tcggccgcgc gccggacttg ctgccccagc
     1501 tcgagcaaat cgccggagaa tcgacggagg cggacgaact ggcggcggag cggctgcgtc
     1561 tatgcatgga ggtgatgagg tgggaaagcg gcagagtgat aactgcgccg gagcgcgagg
     1621 cagctcggcc ggaaagcggc gcttctccgc aaaaggtggt tccggaagag gctcccggag
     1681 cgtcgccccc ttatcaccct atcaccgaat cacgttgtca cgatcactcc ggcccgcccg
     1741 ccatcgtcgc cccgatcatg gcccttatgc agaccgtgcc gtcgccggag gccatcgcgg
     1801 ccacttcgct ggcgctggac gtcgggctga cgctcgagcc ggcagagctg gcgcgctggc
     1861 tgagcgagca tggcttcagc cgctgcgacg cggtggacgt tccgggcgac tttgcccagc
     1921 gtggcggcat tgtggacgtc ttttcgaacg cgcacaccga tcccgtccgc atcgagtttt
     1981 tcggcgacca gattgagtcc atccgcctct ttgacctggg cacgcagcgt tcgtcgcagc
     2041 ttctgcagtc cgtgcaaatt ccggccatgt cgctgccgcg gctgcggcgg gcggacccgc
     2101 gccgcggcct gagcacgagc tttctggcat ttctgccggc ggacacgctg atcgcgctgg
     2161 ccgagccggc ggaggtgcag gagctgggcc gcacatactg gcagcggctg ggggagccgg
     2221 ccgggatggt gccggttgag gcggtttttc gacgggcggc cgacttctcg cagttgcact
     2281 tgcagcgttt caagggcggc gggccgcgga ccgtcgattt cgccgtgcgc agcctgccgc
     2341 aattcgagcc caaatcgacc gaggcattgc aggcgctggt ggagctggcg cgcgagtgcg
     2401 agacgtacgt cctatgtgac aatccggccg aggagcaacg gttcatcgaa ctgctggacc
     2461 agttgccgga gcggtccgcg aacctgcata ccgccgtcgg cgtgctgcac cgcgggtttc
     2521 aatgggggca cgcggcggaa ggggggcaga ctacgcccgc gcagcagacg gcgtacgtgc
     2581 cgcatcacga gctgttccat cgctatcgcc agcggcggac cctgcggcgc gtcgcgcccg
     2641 cgcggccgat cgacagcttc ttcgacctcg acaccggcga cctcgtctta catacgctgc
     2701 acggcatcgg gcgttttctg gggctgaaga cgctggaccg cgccgggcgg ccggaggagt
     2761 acctggcgat cgaattcgcc ggcaaggcgg tggtgcacgt cgcgaccagc cagattcacc
     2821 tggtgcaaaa gtacatcggg gcggtgcgcg gcaagccgaa gctgagcacg ctgggcgggt
     2881 cagcctggaa aaacaccaag gcgcgcgtgg ccgaggcggt gaccgacctg gcgggcgaaa
     2941 tgctggcgat ccaggccgaa cgcgagacgc agccgggcat cgcgtatccg caggacacgg
     3001 cctggcaacg cgagttcgaa gagtcgttcc tgttcgaaga aaccgaggac caactccggg
     3061 cgctggagga catcaagcgc gacatgctgc ggccgcggcc gatggaccgc ctgctctgcg
     3121 gagacgtggg ctacggcaag accgagttgg cgatccgggc cgcatttaag gtggtcgaat
     3181 acggcaagca ggtggcggtg ctggtgccga cgacggtatt ggcggagcag catcaccaga
     3241 catttcgcga gcggctggcg gattatccgt tctcgatcga ggttctttcg cgctttcgca
     3301 ccaagcgtga gcaggaccgc atcgtcgagg cggcccgcaa agggcgcgtg gacattctga
     3361 tcggcacgca ccggctgctt tcccccgacg tgcgtttcgc ggacctcggc ctggtggtga
     3421 ttgatgaaga gcagcgtttc ggggtggacg cgaaagagcg gcttaagcgg ctgcgcgtga
     3481 cggtggacgt attgacactc agcgcgacgc ccatcccgcg gacgctgcac atggcgatgc
     3541 tgggcattcg cgatatttca tcgctggcga cgccgccgct ggatcgacgg gccatcgtga
     3601 cgcaggtgcg gctgtgggat gacggcttga ttcgcgaggc ggtgctacgc gagctcaacc
     3661 gcgacgggca ggtctacttc gtacacaact tcgtgcgcga cattcacgcc gtcgccaatc
     3721 gcctacgcag cattgtgccg gaggcgcggg tggtaatcgg gcatggccag atgtccgcgc
     3781 acgagttgga agacgtgatg ttgaagttcc tgcggcgcga ggcggacgtg ctggtgtcga
     3841 cgaatatcat cgaatccggc ctggacattc cctcggccaa taccatattc gtgaatcgcg
     3901 ccgagcgttt cgggctggct gatctgcacc agcttcgcgg gcgcgtgggc cgctccaaga
     3961 accgcgccta cgcgtatatg ttgctttcgc cgcagcatcc ggtgaaggat acggccgcca
     4021 agcggttgaa ggcgatcgag cactacagcg aactgggcgc tggatttcag atcgcgatgc
     4081 gcgacctgga gattcgcgga gccggcaatc tgctgggagc ggaacagtcg gggcacatcg
     4141 aggcggtcgg ctatgagatg tactgccaga tgctcgagca ggcggtgcgg cggatgaaga
     4201 acgagccggc cgaaccgttc aagccggtga atttggagct gggcgtgacg gccagcattc
     4261 cgcgggcgta catccggtcc gaccgccagc gtatggaagt ctacaagcgc tcgacgcact
     4321 gccgcacacc ggaggatttg gcggtattgc acgatgatct gcgggacgcg ttcggtccgc
     4381 tgccggagga agtgcagacg ctgctgaaac tggcggagct tcgcgttctg gcgcagcgtt
     4441 gggggatccg gtcgattgtg ctcgatccgc cggacgtcat tttcgcggtc gaagattttc
     4501 agaaagccag gccgcttttc gaaaccggcc ccggctcgcc gcgcgtacct gacccgcaga
     4561 cagttcactg gcgtttgccg aagcgctttc ttgaaatgcc gacgctattg acaatcttac
     4621 gccgtcaact ggcgggcgaa gccaaagaag ctatccccca gggcgacccg aacgagccgc
     4681 caccgtcggc tcccccggtc tcacgtggcc gcaagcgcgc cggtgccagg cggttgtaac
     4741 tccttcagag cgcagggcct ggcagacgcg ctgtgcgcgg cccgccatgg ggcttgccaa
     4801 tcggtctctc cagcttatcc tgctgcccgg tgagcaggag tttgaggcat gctttcgatg
     4861 cgattgcgcg gaatggtgtt agcggcggcg ctggccggcg cctattttgg cgggacttcg
     4921 ttcgcggata cgctcgagtt tgccgatggg cgcaagctcg agaattgcta cgtgcgcgat
     4981 gaggggacgc gcattctcgt ctggcggcag ctcgccgacg tggggcagcc gccggaggcg
     5041 ttcccacgca gcgcggtcaa gagctatcag attgagcgtg gcccggagtg ggaccgcaag
     5101 cccgacctgc ccgatctgag cgtcacgttc atcgaggtcg atcccaagaa ctacagcctg
     5161 cacggccgga ttcaggtcga ccagttcggg cgcccgctac caaccggtgc gccggtccta
     5221 aagagccagg gcatacgcga ttacatggaa cccaacaaaa tcatcgcggg cgtccgcacc
     5281 aattatcgcc ccggcgagga actgacgctt acggcccacg tgaagaacgt gggcttcgcc
     5341 ccggccggcc cgttccgcta cacctgcctg gtagatgatg agccgattct ggaggggcgc
     5401 ttcaccgacg cactcaacga gaaccaggaa gtgaccttcg ccgcgaagtg gaagtggcgg
     5461 acgggccagt acacggtccg ctttcgcata gagccggagc acccggaaat cgccaccatc
     5521 aacaacgaaa cgatcgcgcc gctctggggc tgggggctga tctacatcgt caacccgggc
     5581 cgcgtcgccg cttggcgcga gtaccgcaat gcgtacggca ccttttcttg ggaagactac
     5641 taccgctggc acatcgatct gatgaaccag ctcttcgccg atagcgttta cccggccgcc
     5701 cccgagggca tcaaggcacg cgtccggctc gaccgcatcg tttacgccac cgacgtgccg
     5761 gccgccgagc aagcccgctg gaaagagaac ggtctggccg acgatcaggg ggcccgtgta
     5821 tggcacgaca gccccgaaga gttggccagt ggcaagtggg cgccgcccga gcacgccgcc
     5881 cgcaaccaga ccgaatggac gctgcttcac gatctcggcc atgaactcgg tctgccggac
     5941 taccaaacgc tggattacgc cggcgccgag ccgcacgttg cccccgacac cggcgacaag
     6001 atcggccact ccgcgcggta ccccacacaa atgatgtatc gtcctgggcc gtggctattt
     6061 ggcgaggccg atgccggcta tttgaacttc agcatagaca agccgcgcgg ctatcgcggc
     6121 gatatgttct tcgcgctgcc gcgcatcctt gcggtgcgcc tcacggatat gagcgggcgg
     6181 ggcgtgccag gcgccttcgt cgagttcttc cagcgcggcg tggtggtcga acacgagaag
     6241 caggcgggcg gtatcgagga tttgaagtac tggtctgttg tcgaggacgg caacttcgat
     6301 cacccggtct ccgcgtaccc ggtgatgacc ggcaggtcgg accccggcgg cgtcgttcgc
     6361 ctgattccgc gccccgctga gtcgattcgc acgttgaacg gcttccagcg ccgcctgaac
     6421 ggcttcggca acatcgaccc ggagggcaaa cgcggcttaa tgctcgtccg cgtgaccaaa
     6481 gatgaccggc aggcgcattt ctggctggac atcacagagg ccatcacggc ctggttccgc
     6541 ggcgaacagg aatattatga attcgcactg caaacgccgt tcggttcact gggcgcgccg
     6601 cccgcgccgc gggacgtgaa ggcggagcgg aagggcggtc acgaggtccg aataacctgg
     6661 gcgccgccgt cagccgaacg cgagcaacag taccttcagc acgtcgttgg ttatcgggtg
     6721 tacggccgag ttggcaacgg aggtttgaac gaccagccgt ggttcccggt tgccgtctcg
     6781 cctcccgagg gccttagcgt cactgtggac atgcgccagt tcccggtgaa gttcgagcac
     6841 ggctcggtcg tccggcttgg cgtagcgagc gctggcgagt gttctaacgt gagcgaactg
     6901 gtcgaagtgg tgttgccggt ggccactgcg gccgctgggg aatagcggca aaggcgctaa
     6961 cgagcgttca taacagcttg gttgcgcccc agcgctttcc tatagtgcta ctataggtat
     7021 gtcgctttag gcgaatcagg ccagtcatct ggtcgatctt gcctgaggcc ggagccggtg
     7081 cggaaggaca gtccgtgccc ggccaaagtt atcggccgtg catcggtgtg gcggaaagga
     7141 gaccccgtgc gaattctcag tgtctggcgt tggacgctgg tggcggtgtt tgtttgcgtg
     7201 cccgttctgg caggggccgg ttccccgcaa gttgtggacg tgcgggtcct ggagaattcg
     7261 cccgagcgga tcacggtcag ctatagcctc ggggactacg aactgcgcaa cctgacgatt
     7321 gacggccagg actggagcgt gccgcggctg ccgggcgagg cgatcatgct ggaaaagggg
     7381 gcgccgagcc tgcccaaggt gtgtcgcagc gtgatcattc ccgacgacgc cgccatgagc
     7441 gtggccgtgg ttgcagcgga ctactacgac gtcgcctgcg aagtcgcgcc gtccaagggc
     7501 agcttaaaac gcaatgtgaa ccccgcgtcg gtgccttacg agttcggccc ggcttacagc
     7561 cagaaccgat ttttcccggg cgaggtggtt aagcttgggc aaccgtacct gctgcgtgaa
     7621 ctgcggggtg tttgcatcga gctgtacccg tttcagtaca atccgggcag cggcattctg
     7681 cgcgtctata cagatgtgac ggtagagctg acgccggccg gtccgggcaa ggtcaacgtc
     7741 aagcatgccg gccgggactt caagaagccg acacgtgcct tcgactcgat tcattcggcg
     7801 catttcctga atttcggccc gcggctgcgc tatgcgccgt tggacgagca gggcgacttg
     7861 ctgatcatct gtcatgacgc ctggatcccc tacgtacagc cgctggccgc ccacaagaac
     7921 cgtattggca taaataccac agtcgtgggc gtgtcgagca ttggcctcaa tccggacgcc
     7981 atcaagagct acatacagaa cgtgtacgac accagcgatc tggcttttgt gctgctggtc
     8041 ggcgactcgg cccaggtccc gacgccgatt gcgtacggcg ggtcgtccga cgccacttat
     8101 gccaagctgg ccggtgatga caactatccg gacatcgtgg tgggccgctt ctccgccgag
     8161 agcgccgcgc atgtgcaaac ccaggtgctg cggaccatcg aatacgaaac gatgccggcc
     8221 acgcgacagc cgtggttcaa acgcgccgta ggtattgcat cctcacaagg cgacggcgat
     8281 ggtgatgagg gtcaggccga tttcgtccac atgggcgaga ttcgcgactg gctgctggac
     8341 tatgactaca ccgaagtgga tgaaatctac gatcccggtg cgtccgccgg cgcggttacg
     8401 gcggcggtga acgccggccg cggcattatc aactattgcg ggcacggcac tcgcagcgcc
     8461 tggacgacta cccgcttttc cagcacgcac atcgccgagc tgaccaacta caaccagctt
     8521 ccgttcatct tcagcgtggc ttgcgtgaac ggccagttcg ccaacggcac gtgttttgcg
     8581 gaaacctggc tgcgggcgac gcgcgacggc gagcccaccg gcgccgtggc ggcctacatg
     8641 tcgtccatca accaggactg ggcgccgccc atggaagctc aggacgagtt caacctgcta
     8701 ttgactcgcg aagattattt cagcttcggc gcgctgtgct acgccggttc gtgcgcgatg
     8761 atggacagtt acggctcgac gcccggctcg gatggcctgg tgaacttcga cacctggatc
     8821 ctcttcggtg acccgtcgtt gcgcgtcttc ggctacgttc agccgcggca ccccgccggc
     8881 gatctgaact gcgacggcgc ggtgaacgcg ttcgacattg atccgttcgt gctggctttg
     8941 accgatccgg ccggctacgc cgcggcctac gctgagtgcg acatcaacaa cgccgacatc
     9001 aacaacgatg gcacggtcaa cgccttcgat attgatccgt tcgtggcagt cctgaccggc
     9061 ggcggctgat tcgccgcgcg gcccgaccgg ccgggcgggc cctgtattgc ggagcaggat
     9121 ggagatcgac gggcggacac ctcgcccgga gggcgaacga ttgttgccag gcgtttcaac
     9181 gcctggaaaa agccacccct caacttctct tcccctcctc ttccgcgccg ggagggcgag
     9241 cgattgttgc caggcgtttc aacgcctgga acccggccca tgcccctccc gcaccccagc
     9301 cgtcctcgct cagagggcga acgcttgttg ccaggcgttt caacgcctgg aaaaagccac
     9361 gccttaactt ctcttcccct cctcttccgc gcccggaggg ccagcgattg tcgccaggcg
     9421 ttataacgcc tggaacccgg cccacgcccc tcccgcaccc cagccgtcct cgcccggagg
     9481 acgaacgatt gttgccaggc gtttcaacgc ctgcagcccc gaaccggttg ggtgttaaca
     9541 acttctgact gccgtgaaac aagaaaacga ttccggcagc acgcggatca ggaacagccc
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgactgccgt gaaacaagaa
     9721 aacgattccg gcagcacgcg gatcaggaac agcccgggtg tgaaacgggg acgcagctag
     9781 tcccaaaaca agcgtagatg aaagcctgtt tatcctcatg agggcgatcc gcggccgggg
     9841 catgtgcccc cgtccgaccg cgatcgcggt cgggattgct gtgccgagtt gcttgctcca
     9901 ttcgcgtccc tcactttcct catctctggc ggccgcggct aaccgggacc tccatatccc
     9961 ggtgtcaaaa ttgtggtgtc aacaccgcag cgggcacccc aacgccacca ggccgcgggc
    10021 aaacaccgct tgccacgggt tccaggccag caggcggtac acgatccgcc ggccagcccg
    10081 cacaatctgg cacggcaccc gcacgaagga gttaacaaaa cgcttgaact ccatggttaa
    10141 cacctggcgc ttctgctggc ggtgcttctc tttccagcgg ccgcgctccg gcaggcgcaa
    10201 cgcgaaccag gctttcagcg accacgccac actggccatc accatatagg cccagttgct
    10261 gaccagccca tccaccggca tgttcaacgc ccgcacgccg ttgcggagtt gctccagcag
    10321 gttctcctgg ttgcagcggt cattggccag gtacaccagt tccgccggat cgcccgccgg
    10381 cagattggtc aggtagaaga agtagcgcac atcctcgaaa aaacggcact gcccgtgctc
    10441 aaccgccagg tccttccgca gggcgaccac cgtgtattgc cgcttacagg catatggctt
    10501 ataggcaaaa caggccacgg cctcgccctg cagcttgatg ttcctgtagc cgttggcgac
    10561 gacgatctcc tctttgacgt tagtcggccg ttgccgcggc ttggtgctta cctcgtattt
    10621 cggcgggcgt tgcagcgttt tccaggccga ttcaggcagg ttttcggcca gttccaccag
    10681 gttggcgcgc gcatccaggc cgaacaggaa ctgcactccg tccagatgcc agcggtccaa
    10741 gtgctctgtc tgggagaagt ccgtgtcgcc gcgcaacagc acacgtttga agcccgcccg
    10801 ccggcacagg gcaatcgccc ggtccagata gcccgccgcg ccttcgtttg aaggccggtt
    10861 gccggagcga ttggccaggt ataacggctc ctgggtattg gccagcgaga ccaccagtgg
    10921 gtggtagccc caaacgccgt tgtaagcccg gccgatgccc tgtttgcatt cgccataggt
    10981 ttcggccact accccgtccg cgtccaggat ggccagtttg aagaactcct tcggctgctg
    11041 acgccacaca cccagtcgcg cctcgttgag cgtgtcctgc agaatctcta cgtcatgtgc
    11101 ggtgaagcgc cgacagaagt cgccctccgt tgtgggatcg ggaatgcgct gggcgcccag
    11161 cgcgtccagg tagacttcgt cgttgcgcca caattccagg tcttccaggc agctgccgcc
    11221 cgccagaatg ttgtaggcca cgttgaggac atggtcggat tcatggtagg gcaaatgtat
    11281 cttgagcaag tgcaggcggc ggtcgatggc ggcgatcaat ccggcatgcc gcgccagatg
    11341 atgcatggcc ccgatgccgc ccggggccag gccccgcagg cggtcgctca actcgtaatg
    11401 aatattgcgg gcgcggaaca tgggctggtc ctgcggagcc cacgtacgct cgcgcaggcg
//
LOCUS       DAOSYS010000108        11427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4830_length_11427_cov_5.448030, whole genome shotgun sequence.
ACCESSION   DAOSYS010000108 DAOSYS010000000
VERSION     DAOSYS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4830_length_11427_cov_5.448030"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..580)
                     /locus_tag="PLU99_08205"
     CDS             complement(179..580)
                     /locus_tag="PLU99_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17078.1"
                     /translation="MNNNVAEKYMQARRELIARLGEIENELQQIKSALGLEANGQARF
                     APAGRGAPHGGGKRKGKRGSGLLAILRTLNDNGPIKPTKVAQLSGVRPTTVSNALSTL
                     KNKRLTARSDRGWTLTPAGKAELAQREKQMA"
     gene            complement(942..3293)
                     /locus_tag="PLU99_08210"
     CDS             complement(942..3293)
                     /locus_tag="PLU99_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOO17079.1"
                     /translation="MNFKTTGLLVALVVVLGAVWVFYPKSTAPDETAVTVAPPSKQDT
                     QPLLSPAPESKSIVRVAVERPNKPRLVFTRAPKAEQPDQMENWQADEPLAVPVQTYTV
                     DGLVSTMIGLQTRGSFKPGEAGGPTEADAGLNPPLATVTLTDKNGREYKFEVGQKAAM
                     STDTYLRLAGEGYIHIVSRELESQVKREFDEYRDKKLLTLNPSNAVRLAMQVEGKPYE
                     FARGADSQWVIDAPIKSHAEREPVMELLRKLSGLRAVEFLSGSNVELASYGLDRPYLV
                     ADVTTETKKTKPQPVDESASQPAEPEEETITEQVGLQIGGFADLKKENRYVKLAGQDW
                     VATVAQKDVAPLVPDLAKLRDSRVTRVAPNMAQKLSLSAEGMTVVLEKTGGKWQGQGE
                     LADLEPAAVNDLLQAFEDLRAIDYIDEPNDLAEYGLAEPRAVVSVTVSGSVEPVTLRI
                     GNLTKSGRNAYVQREGEPTVFVVSAQQAYRMVVPPLTLRSRKIVSIDEDNIERLVIER
                     GSTRYELVREEDQWKPAALDVPIDEGPVRAIVNDLSHLRARKVVARGDEGRYGLDQPE
                     LTIHFAATEAPAGEKATTQPEAGPPAPPVVNEHTLRVSRREKTAFARFDDDPYVYELD
                     DTVYRVLSAELLNRKLFPFAADEVVGVTVVQPSGTLEFAKQDDQWVFTPDPFVKIAQD
                     KVNELINTLSGLRAESFVQYRGADFQANGLFTAPLTTSLKLKNGELANLKIEQKTDDT
                     SPLAGWVEQKCAFRLAPADYDKLIRTLDHYLQGETFKTDAAKP"
     gene            complement(3299..5491)
                     /locus_tag="PLU99_08215"
     CDS             complement(3299..5491)
                     /locus_tag="PLU99_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17080.1"
                     /translation="MTSSKRRFLFGFNVAVQSLLVLAVVIGVVWIAKRFNAQADWTSM
                     GINSLSPRTEQMLRGLDQNIYITALFAKPDKERDKIQYKRWQELKDLMDLYQSVGGAR
                     VTTRIIEPNLDKAETDKLLDRLKKLPVYRDEAQPHKEALEKQLPPLSEKISALASAEL
                     QQLEALQGSEPALARNRALNIIRNHWQKILQDSNEIPELLQELERAEVPRYGRAIKSV
                     RDFLEATKLRAEDTAGWMTGEGLSVAGLTPDARTFFEESKTRYAEVLAEADSLLEATK
                     DLKEVKLEEIHSELTRWQHGPPVLVESEKEARVVSNYELWQPPKDRHAPVGPEGDDRV
                     FAGESAISSAILKLTQKDKAAVVFTRFGGTSPIKPDFSQMNFMNMRQMPTAPYQELAQ
                     ILEQENFVTHDWDVSKEKTPPTVEGTTRSIYVVFPPEPPPRQNPMEPSPQQGITPADR
                     QIILDAVNASGAAIFLAGYEQPAGPFGGPSGGYEYAEYLKSTWGIEVDSRYVAWRFTP
                     NPQRPGWWAPAARDPILSTGGGPESPVRLTDHPITEPLQNDRAAFYAACPVRVSEAAS
                     RPAGLEVQVLAEVRDTQDVWALGDLMRVSEEIKRNQGCTRPSEDDVRAPFALAVAASD
                     ASGKKVVVFGSEQFMADNVAQASGFMQIGNALVIGQLYPANTDIFVNTLHWLTGESNR
                     IALGPRGEVPRLTKLDEAWANRLPWLLLGIWPASALLVGMCVWFVRRR"
     gene            complement(5500..6219)
                     /locus_tag="PLU99_08220"
     CDS             complement(5500..6219)
                     /locus_tag="PLU99_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17081.1"
                     /translation="MRNVVAVMQRELLSLFWSPIGYIVITGFWLVTGVLVCATKAFTP
                     GQPAMLRDVFMWTPFVLTLIIPAISMRTLSEEYRTGTIESLMTAPVTDTQMVLGKYLA
                     SYIFYLVMIAGTLIYLVLMAIFGDPDWGSAAAAYLGLILVGLAFTAFGVFASSLTHNQ
                     IVAWILGAVPLMLVAWFAYFIVGQVEGWMREVLQQVNIMGRFGEFARGQVALDGVVFF
                     IAAAFLFLFLTVKVVESRRWR"
     gene            complement(6251..7186)
                     /locus_tag="PLU99_08225"
     CDS             complement(6251..7186)
                     /locus_tag="PLU99_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17082.1"
                     /translation="MIEVRNLSKYYGAHRAVDNISFTVADGEIVGFLGPNGAGKTTTI
                     RILTCFQPATSGSATVAGHDVFTESLAVRAAVGYMPENVPLYPEMRVREYLRFRGKLR
                     GLDGAAREAAIDRVTQRCWLQDVINRPISQLSKGFRQRVGLADALLHNPPVLILDEPT
                     VGLDPTQIRETRSLIRELAQDHTVILSSHILPEVEATCQRIIIIHKGKLVASGSPSEL
                     RERISEGAKVIAEIKGAPDEIGAAVRQLPGVTDVQAERRDGWTRLAVVAASDLRETIA
                     QTAFARGWAVRELHRDAASLEDFFVKIVTGAHEQE"
     gene            complement(7654..7839)
                     /gene="rpsU"
                     /locus_tag="PLU99_08230"
     CDS             complement(7654..7839)
                     /gene="rpsU"
                     /locus_tag="PLU99_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOO17083.1"
                     /translation="MIRVRVRSNESVEQMVRRFKKLCEKEGLTRDIKRTSYYEKPSER
                     RRRKARKSLKRIIREQF"
     gene            complement(8064..9227)
                     /locus_tag="PLU99_08235"
     CDS             complement(8064..9227)
                     /locus_tag="PLU99_08235"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO17084.1"
                     /translation="MPPGDPQATELLQQSQKALRALSAIAYDGEYVAEGIAAAVLPSR
                     KGHVVLARNNSRFLRMRASEWTPAPDGRLVLTAEMISDGTVVASINHDRQLYREFTGP
                     GAAGEVLRWHGIVLEQLLRGELYKNEIANGTANFKGSEVVDGIDCHIIEAPHPEASVV
                     VRWSIGKEDLLPRRVVRIISGEIGEGRGDLRLNSLDPNPPAGPELFVFAKPEGYSDAG
                     PTVGARTVPSPLEAHFLPVGSEAPDWTLGRPGGGEVALKDLRGKVVFLSFWASWAPPC
                     VNALPGIQRVCEAYKDRPFAAYVINSYEYRSNPAQALPAELSQTLLVGGDAVAKAYRA
                     QGIPTFYVISPEGKVLFVEGGTLHENSIKAAIDKALQGLGQPNPAAAPAAPGT"
     gene            complement(9641..10732)
                     /locus_tag="PLU99_08240"
     CDS             complement(9641..10732)
                     /locus_tag="PLU99_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO17085.1"
                     /translation="MARKAAVRYAYEPDYAVPPGATLQETIDSLGMTQRELALRTGMA
                     PKTINEIINGKAPITPATSVLLERVTGVPARMWNNLESNYREQLARTADRERLQGDLD
                     WLDRVPTGELVKRGVITPQPDRPSLLHAVLRFFGVGTVEQWQQLWMQPAGAFRKSPKF
                     AAQPEAVATWLRLGELQAQEIQTQPYDKARFRAALDEIRRLTVEPPERFQARMVELAA
                     AAGVAVVFVPEIRKCPVSGVARWLTPDKALIQLSLRYKTDDQFWFSFFHEAGHVLNDP
                     KKEVYIHDDDDLDGREQAADRFAATFLIPAEHSAELHELTTREAIVEFAQRIGVAPGI
                     VVGRLQKQRLVAWASNLNHLKRRFRWNRA"
     gene            complement(10782..11120)
                     /locus_tag="PLU99_08245"
     CDS             complement(10782..11120)
                     /locus_tag="PLU99_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killer suppression protein"
                     /protein_id="HOO17086.1"
                     /translation="MEVSFATRKMQKGCSSDKAMRRQWGDRLAQKLQQRLAELAAADS
                     LADIARLPAARCHELTGDRAGQLSVDLSHPYRLLFAPGNEPIPCKDDGGLDWTKVTKV
                     VVLEVCDTHE"
BASE COUNT         2057 a         3915 c         3258 g         2197 t
ORIGIN      
        1 acatactcca cccagatgcg tcattgtagt agctgcggcg gagttttgat cagctcggcg
       61 gccacgttcg ctgggcttga agcgccgagg cacgcgtggc cgggaaccgc tacggattcc
      121 cggccacgct atggcgggta aatactcatg tgtccccccg cccgatccaa cgaggggatc
      181 aggccatttg tttttcgcgc tgcgccagtt ccgccttgcc ggccggcgtc agtgtccagc
      241 cgcggtcaga gcgcgcggtg agtcgcttgt tcttgagtgt gctcagggcg ttggagacgg
      301 tcgtggggcg gactcccgac agttgcgcga ccttcgtcgg tttgattggc ccattgtcat
      361 tcagcgttcg caggatggcc agcaggccgg atccgcgttt gcccttgcgc ttgccacctc
      421 catgcggcgc gccgcgcccg gccggggcga accgcgcctg cccgttggcc tccaagccca
      481 gggccgactt gatctgctgc aactcattct cgatttcacc cagccgagcg atcagttccc
      541 ggcgggcttg catgtacttc tctgcgacgt tgttgttcac ggctcggtcc ttccaccccg
      601 aaaccaaccc cttccagccc tcgattaatg agaaaaatac ccgcaaagcc gttgaacgtc
      661 aagcgccggc actgacacgt cgtgccagtg gtctattgca gaattgacta ccgctctcga
      721 atatgcaaga gagtccgcgc aacacttcta gcgtcggatc cggccaccag tccccaaact
      781 cttcatccct ggcgaatctt cgtcagaaga ccgccaccaa tacacgaact ggccaccgca
      841 gtacaactgc gatggccagt agaacgactg tcttgtttac ttcagaaccg ccggcaatgt
      901 agccttcggt tgcgggggcc gggggcgctt cggaccggcc tttaaggctt agccgcgtca
      961 gtcttgaacg tctcaccttg caggtaatgg tccagcgtgc gaatcagctt gtcgtagtcg
     1021 gccggcgcca gccggaatgc gcacttctgc tcgacccagc ccgccagggg cgacgtgtca
     1081 tccgtcttct gttcgatctt taggtttgca agctccccat tcttgagctt caggctggtg
     1141 gtcagcgggg cggtaaacaa gccgttggct tggaagtccg cgccgcggta ctgcacaaag
     1201 ctttcagctc gcagtccact caaagtgttt atcaattcgt tcaccttgtc ctgagcgatc
     1261 ttcacaaacg gatccggcgt aaatacccat tggtcgtcct gcttggcaaa ctccagtgtg
     1321 cccgagggtt gaaccaccgt tacgccgaca acctcgtccg cggcaaaggg gaagagctta
     1381 cgattcagca attcagccga caacacgcgg tagaccgtgt cgtccagctc gtatacgtac
     1441 ggatcgtcat cgaaacgcgc gaaggccgtc ttctcccgcc ggctgacgcg cagcgtgtgc
     1501 tcgttgacta ccggcggcgc cggcggccct gcctcgggct gcgttgtcgc cttttcgccg
     1561 gccggtgctt ccgttgctgc gaagtgaatc gtcagctcag gttgatccaa accgtaccgg
     1621 ccctcatcac cccgcgccac aaccttacgc gctcgcagat gagaaaggtc gttgacaatg
     1681 gcccgcaccg gaccttcatc gatcgggaca tccaatgccg ccggcttcca ctggtcctct
     1741 tcgcgaacca actcgtagcg cgtgctgccg cgctcaatca ccagccgctc tatattgtcc
     1801 tcatcaatgg agacaatctt gcgcgagcgc agcgtcagcg gcggaacgac catccgatac
     1861 gcctgctgag cgctaaccac gaacaccgtc ggctcgccct cacgctgtac gtacgcattg
     1921 cgcccgctct tggtaagatt gccgatccgc aacgtcaccg gctccacgct gccgctgacg
     1981 gtgactgaca ctacggcacg cggctccgcc agtccatatt cggccaaatc gttaggctcg
     2041 tcgatgtaat cgatcgcccg cagatcctca aaagcctgca gcagatcgtt caccgcggcc
     2101 ggctctaggt cggccaactc accctgaccc tgccacttgc cgcccgtctt ctccagcacg
     2161 accgtcatac cctcggccga tagcgacaac ttctgggcca tattcggcgc gacgcgcgtc
     2221 acgcgcgagt cgcgcagctt cgccaaatcc ggcaccagcg gcgccacgtc cttctgggca
     2281 accgtcgcga cccaatcctg gccggccagt ttgacgtacc ggttttcctt cttcaggtcc
     2341 gcaaacccgc cgatctgcaa acccacctgc tcggtgatcg tctcttcctc gggttcggcc
     2401 ggctgcgacg cactctcgtc caccggctgc ggtttggtct tcttggtttc agtcgtgacg
     2461 tccgccacca gatagggacg atccagcccg taactcgcca gctcgacgtt ggagccgctg
     2521 aggaattcga ccgcccgcag accggacaac ttgcgcagca gttccatcac cggttcgcgt
     2581 tcagcatgcg acttaatcgg ggcgtcgatc acccattgac tgtctgcgcc gcgtgcaaac
     2641 tcatacggct tgccctcaac ctgcattgct agccgcactg cattgctggg gttgagcgtc
     2701 agcagcttct tgtcgcggta ctcgtcgaat tccctcttga cctgcgactc cagctcgcgc
     2761 gagacaatgt ggatataacc ctcgcctgcc agccgcaggt acgtgtccgt gctcatggcc
     2821 gccttctggc cgacttcgaa cttgtactcc cgcccgttct tgtcggtgag cgtcaccgtg
     2881 gccagcggcg ggttaagccc cgcgtccgcc tcggtcgggc caccggcctc gcccggcttg
     2941 aacgaaccac gcgtctgcag gccgatcatg gtcgaaacca gcccatccac cgtgtaagtc
     3001 tgcaccggca ccgccagcgg ctcatccgcc tgccagttct ccatctggtc cggctgctcc
     3061 gcctttggcg cgcgggtgaa taccagccgc ggcttgttcg gccgctctac agccacgcgc
     3121 actatgctct tgctttccgg agccgggcta agcaagggct gcgtgtcctg tttgctcggc
     3181 ggcgctacgg taaccgccgt ctcatcgggc gcggtcgact tcggatagaa aacccagacg
     3241 gcgcccaaca ccaccaccag cgccaccagg agccctgtag ttttaaaatt catccctgct
     3301 atctccgtcg cacgaaccaa acgcacattc cgaccagcag cgccgaggcc ggccaaatgc
     3361 cgagcagcag ccacggcaga cggttagccc aagcctcatc cagtttcgtc agccgcggca
     3421 cctccccgcg cggtccaagc gcgatacggt tggactcgcc cgtcagccag tgcagcgtgt
     3481 tgacaaaaat gtcggtattg gccggataaa gctgaccaat gaccagcgca ttgccgatct
     3541 gcatgaagcc cgaggcctga gccacgttgt cggccatgaa ctgttcactg ccgaaaacaa
     3601 cgaccttctt tcccgatgcg tcactggcgg ccaccgccag ggcaaagggg gcccggacgt
     3661 cgtcttcgct cggccgcgtg cagccctgat tacgcttgat ctcctcgctg accctcatca
     3721 ggtcacccag ggcccacacg tcttgggtat ccctgacctc cgccagaacc tgcacctcca
     3781 gacccgcggg ccggctggcc gcctcgctca cgcgcaccgg gcaagcggcg tagaacgccg
     3841 cacggtcatt ctgcaacggc tcggtgatcg ggtggtctgt caaccgcacc ggactttcgg
     3901 ggccgccccc tgtactcaga atcgggtcac gcgcggcggg cgcccaccaa cccggcctct
     3961 gcggattggg cgtaaaccgc caggccacat acctcgagtc cacctcgatg ccccaggtgc
     4021 tcttcaggta ctcggcatac tcgtaacccc cggaaggtcc gccgaacggg cccgcgggct
     4081 gctcgtagcc ggccaggaag atggccgccc cggaggcatt caccgcgtcc agaatgatct
     4141 gccgatcggc cggcgtgatg ccctgttgcg gcgacggctc catcggattc tgccgcggcg
     4201 gcggctcggg cgggaagacc acgtatatgc tgcgggtcgt gccttcaacc gtgggcggcg
     4261 tcttttcctt ggacacgtcc cagtcgtgcg taacgaagtt ctcctgctcg agaatttgag
     4321 ccagttcctg gtaaggcgcc gtcggcatct gccgcatatt catgaagttc atctgcgaga
     4381 agtcgggctt gatcggcgag gtcccgccaa aacgcgtgaa taccaccgcc gccttgtcct
     4441 tctgcgtcaa cttgagtatc gccgacgaga tcgccgactc gcccgcgaac acccggtcat
     4501 ctccctccgg cccgacgggg gcatgccggt ccttcggcgg ctgccacagc tcgtaattgg
     4561 acacgacccg ggcttctttc tcgctctcaa ccagcaccgg cgggccatgc tgccagcgcg
     4621 tcagctcgct gtggatttcc tcgagcttta cttccttcag atccttggtg gcctccaaca
     4681 gactgtccgc ctcggccagg acctcggcgt agcgcgtctt ggattcctcg aaaaacgttc
     4741 tcgcatccgg cgtcagcccc gctaccgaca gcccctcgcc ggtcatccag cccgccgtgt
     4801 cctcggcgcg cagtttggtc gcctccagga aatcccgcac gctcttgatc gcccggccat
     4861 aacgcggcac ctcggcccgc tccagctcct ggagcaactc tggaatttca ttggagtcct
     4921 gcagtatctt ctgccagtga ttgcggatga tgttcagcgc acggttccgc gccagagccg
     4981 gttccgatcc ttgcaaagct tcaagctgct gaagttcggc cgaagccagc gcgctgatct
     5041 tctccgacaa gggcggcaac tgcttctcca gcgcttcctt gtgcggctga gcctcgtcgc
     5101 ggtagaccgg cagtttcttg agccggtcga gcagcttgtc cgtctcggcc ttgtccaggt
     5161 tcggctcgat gatgcgcgtc gtgacccgcg caccgcccac cgactgatac aggtccatca
     5221 ggtccttcag ttcctgccaa cgcttgtact gaattttgtc gcgttccttg tccggcttgg
     5281 cgaacagggc cgtaatgtag atgttttgat ccagcccgcg cagcatttgc tccgtccgcg
     5341 ggctcagact gttgatgccc atgctggtcc agtccgcttg cgcgttaaag cgcttcgcga
     5401 tccaaaccac cccaatcacc actgccaaca ccagcagcga ctgaacagct acattgaacc
     5461 caaataggaa ccggcgtttt gaagaagtca ttggttctgc taccgccatc gccgcgactc
     5521 caccactttg accgtcagaa acagaaacag gaacgcggcc gcgatgaaga aaaccacccc
     5581 gtccagcgcg acctggccgc gagcaaactc gccaaagcgg cccatgatgt tgacttgttg
     5641 gagaacctcg cgcatccagc cctccacctg gcccacgatg aaatacgcaa accacgccac
     5701 cagcatcagt ggaaccgccc ccagaatcca cgccacaatc tggttatgcg tcaggctgct
     5761 ggcgaacact ccgaacgccg taaaggccag ccccaccagt atcagaccca ggtacgccgc
     5821 ggcggcgcta ccccagtccg gatcaccgaa aatcgccatc aataccaggt aaatcagcgt
     5881 cccggcgatc atcaccaggt agaaaatgta tgacgcaagg tacttgccga gcaccatttg
     5941 cgtgtcggtc accggggccg tcatcaaact ctcaatcgtc cccgtgcggt attcctcgct
     6001 caacgtccgc atgctgatcg ccggaatgat cagcgtcagc acgaacggcg tccacatgaa
     6061 gacgtcacgc agcatcgccg gctgcccggg cgtgaacgcc ttcgtcgcgc aaaccagcac
     6121 cccggtcacg agccaaaaac ccgttatcac gatgtagccg atcggcgacc agaaaaggga
     6181 aagcagctcg cgctgcatca ccgccacaac atttctcata gtgcaatgct cctcgcaggt
     6241 cgcttcccgc ttattcctgc tcgtgcgccc cggtcacgat cttgacgaag aagtcttcca
     6301 gactggcagc gtcccggtgc agctcacgca ccgcccagcc ccgcgcaaac gcggtctgcg
     6361 cgatcgtctc ccgcaggtcg ctcgccgcaa ccaccgccaa acgcgtccac ccgtcccggc
     6421 gttccgcttg cacgtccgtc acccccggca actggcgcac ggccgcaccg atctcgtccg
     6481 gagcgccctt gatctccgca atgaccttgg ccccttcgct gatccgttcg cgcaactcgc
     6541 ttggcgaacc ggacgcgacc agcttgccct tgtgaatgat gatgattcgc tgacaggtcg
     6601 cctcgacctc cggcagaata tggctggaca gtatgaccgt gtgatcctgc gccagctcgc
     6661 ggatcagcga gcgcgtctcg cgaatctgcg tcgggtcgag cccgaccgtc ggctcgtcca
     6721 gaatcagcac cggcgggttg tgcaacaatg cgtccgccag ccccacgcgc tgcctgaaac
     6781 ccttcgaaag ctggctgatc ggccggttga tcacgtcctg cagccagcaa cgctgcgtca
     6841 cgcggtcgat cgccgcctcc cgcgccgcgc catccagccc gcgcagcttg ccccggaacc
     6901 gcaggtactc gcgcacccgc atttccgggt acagcggaac gttttccggc atgtacccaa
     6961 ccgccgctcg caccgccagc gactccgtga atacgtcatg gcccgcgacc gtggccgacc
     7021 cagatgtggc cggctgaaag caggtcagta tgcggatggt ggtcgtcttc cccgcaccgt
     7081 tcgggcccaa gaaaccgacg atttcgccat cggccacggt gaaggaaata ttgtccaccg
     7141 ctcgatgagc gccgtagtac tttgagaggt ttcggacttc gatcacgcct ggtcctcaca
     7201 tttgagcatc acgcctgtcc ctggtgagcg tcacgccgtc actggcaagc atcacgcctg
     7261 tactggcgaa aacaccctat agacggtgca ggaggtcgga accggtcgga aatcgtaaac
     7321 aaaccgccct gcctcgcgaa agccgcccta cctcgcgaaa gccgctcgcc gtggctgccg
     7381 caccgtacac gccagtgcga atgcgacgcg aaactcgaat ttaaaagcgc gttaccggac
     7441 tgtcaagaga agtgtcgccc tgcgcccctg ttgcccctcg cctctcaagg ccacgcgcgt
     7501 acctgcctac aggggaaaag ccggggcctt ttcaaacaca tagccactcc cccgcattgc
     7561 gcagagcgcg cgcctgttct gtttccggat tggacttctt ccctattggc cgcgtgtgat
     7621 gcgggtcatc gccggcctgg tccggggccg cgatcagaac tgttcgcgga taatccgctt
     7681 gagtgatttg cgagcttttc tgcggcggcg ctcggagggc ttctcgtaat agctggttcg
     7741 cttaatgtcg cgcgtcaggc cttccttctc gcagagcttc ttgaagcggc gcaccatctg
     7801 ctccacggac tcattgctgc gtacccgcac tctaatcatg cacaccaatt cctttcaaaa
     7861 ccggacccga gttaccttgt cacagccccc cgcgcccatc agcgcttagc gacagcccag
     7921 cattatatgc ccccggcccg ttctcctgca acaccaacgc gtcgcccggc acacgccgcc
     7981 gaaacgggag cgtgcccggc tccaaccaaa cacccgtccc tgcctccacg cccctcaatt
     8041 tcatcgccca atcgaccgcc cgatcacgtt ccgggagcgg cgggggctgc ggccgggtta
     8101 ggctggccca aaccctgcag cgccttgtcg attgccgcct taattgagtt ctcgtgcaag
     8161 gtgccgccct cgacgaacag cactttgccc tccgggctaa tcacgtagaa ggtcggtatc
     8221 ccctgggccc ggtaggcttt cgcgaccgcg tcaccaccaa ccaaaagggt ctgcgagagc
     8281 tcggcgggaa gcgcctgggc cggattcgag cggtattcgt aggagttgat cacgtacgcg
     8341 gcgaagggcc ggtccttgta agcctcacac accctttgaa tacctggcaa ggcattcacg
     8401 cagggcgggg cccaactggc ccagaagctc agaaaaacga ctttgccacg cagatccttc
     8461 agggccacct ccccgccacc ggggcgccct agcgtccaat cgggggcttc agagccgacc
     8521 ggcagaaaat gggcttccag cggcgaggga accgtgcgcg cgccgactgt ggggccggcg
     8581 tccgagtagc cctccggctt tgcgaatacg aacagctcgg ggcctgcggg ggggttcgga
     8641 tccagagaat tcaaccgcaa atctccccgc ccctcgccaa tttcgccgct gatgattcga
     8701 accacgcgac gcggcagcaa gtcctccttc ccgatcgacc agcggaccac gaccgacgcc
     8761 tcgggatgcg gcgcctcaat gatgtggcag tcgatgccgt ccaccacttc agaacccttg
     8821 aagttcgccg tgccattagc aatttcgttc ttatacagct caccgcgcag cagttgctcg
     8881 aggacgattc catgccagcg cagcacctcg ccggccgcgc ctggtccggt gaactcgcgg
     8941 taaagctggc ggtcatggtt gatgctggcc accaccgtgc cgtcgctgat catctcggcc
     9001 gtcagcacca ggcgcccgtc gggcgcgggc gtccattcgc tggcgcgcat tctcaaaaac
     9061 cgcgagttgt tgcgcgccag aacgacgtgc cccttcctgc tcggcagaac ggcggccgcg
     9121 attccctcgg ccacgtactc gccatcgtac gcgatggccg aaagcgctcg cagcgctttc
     9181 tggctttgct gcagcagttc ggtggcctgg ggatcgcctg gcggcaccgg gggcctcgcg
     9241 ggccgagagg ccggcgctcg aacgggctgg ttgggcacgc ccttaggtgg ctgcgaagcg
     9301 gcgggttgcg acgacgaagg acgcgaggtg agttggttcg gaaccggctg atcggcctgg
     9361 gcgcaaatga agaaccccag ccagacaaat acgacagaaa aaacccggaa actcatcatt
     9421 ctctcctcgt tttgtggccc tgcggctcac gaagccggcc gtgtcggacc ctgtaatcat
     9481 gccacccagc cgaagcagtc gcaagaggca tttgacggcg tcccggccgc ggcctggttg
     9541 catgcttggc ggcgccgaaa tcaaagcctg gacgccaccc cgcggcctgc cagcgccgac
     9601 cggccggcga ccgacgcaga atgcaccaag agtggctgct ttatgcgcga ttccatcgga
     9661 accgtcgctt aaggtggttg aggttcgagg cccacgctac taagcgctgc ttctggaggc
     9721 gccccacgac gatcccggga gctacgccga tccgttgggc aaactcgaca atggcttcgc
     9781 gtgtggtcag ctcgtgcagt tccgccgagt gctcagcggg gatcaagaag gtcgcggcaa
     9841 accgatccgc cgcttgttcg cgcccatcca agtcatcgtc gtcgtgaatg tagacctcct
     9901 tcttggggtc attgagaacg tgaccggctt cgtggaagaa cgagaaccag aactggtcgt
     9961 ccgtcttgta gcgcaggctg agctggatta gagctttgtc cggggtcagc cagcgcgcca
    10021 cgccgctcac ggggcacttg cgtatctcgg gcacgaagac tacggccaca cccgccgccg
    10081 cggcgagttc aaccatgcgc gcttggaacc tctcgggcgg ctccacggtc agcctgcgaa
    10141 tctcgtcgag agcggcacgg aacctggcct tatcgtaggg ctgggtctgt atctcctgcg
    10201 cttgaagttc gccaagccgc agccaggtcg cgaccgcttc gggctgcgcg gcgaacttgg
    10261 gcgacttgcg aaaggcgccc gccggctgca tccaaagctg ctgccactgc tccacagtcc
    10321 cgacgccgaa gaaacgcagg acagcgtgca acagcgacgg ccggtcgggt tgaggggtga
    10381 ttacgccgcg tttgaccagc tctccagttg gaaccctgtc tagccaatcc agatcgccct
    10441 ggagacgctc gcggtcggca gtgcgcgcca gttgctcgcg gtagttcgat tccaggttgt
    10501 tccacatgcg cgctgggacg cccgtaacgc gctccagcag gacggacgtg gccggcgtga
    10561 tcggcgcctt gccattgatg atctcgttta tggttttggg ggccatgccg gtgcgcaacg
    10621 ccagttcgcg ctgcgtcata ccgagggaat cgatcgtctc ctgaagcgtg gctcccgggg
    10681 ggacggcata gtccggttcg tacgcgtacc ttactgccgc ttttcgcgcc atgcgctgct
    10741 ccgtgctcac cccgccgtag gagaccgcgt cgcctggcgg tctattcgtg agtatcgcaa
    10801 acctccaaca cgacgacctt cgtcactttt gtccaatcca gcccgccgtc gtccttgcag
    10861 gggatcggtt cgttgcccgg cgcaaagagc agccggtaag gatggctcag atcaaccgaa
    10921 agttgcccgg cgcggtcccc ggtcagttca tggcagcgcg ccgccggcag ccgggcgatg
    10981 tcggcgagag agtcggcggc cgcgagttcc gcgaggcgct gctggagctt ctgcgcgagc
    11041 ctatcacccc actgccgccg catggcctta tcggacgagc agcccttctg catcttccgc
    11101 gtggcgaagc tcacctccat ccggtgtcgg ctccttttag tttacgtatc acgttaacgt
    11161 ttttcaagcc caggtcaagc ttctttacca tgccgcctcg aagaccggcg gtgatttgcg
    11221 aatctgtccc gccgggcagc ccccccgcct tccccagccc tttagcgggc ggttgctggt
    11281 catgatcgtg ggccgcagtt cattcgggcg taaggatcga aacggggatc gcaacgggga
    11341 cgacggatcg aaacggggac gcagctagtt ccctgacgga tcgaaacggg gacgcagcta
    11401 gttccctggc ggatcgaaac ggggacg
//
LOCUS       DAOSYS010000109        11326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4892_length_11326_cov_5.463845, whole genome shotgun sequence.
ACCESSION   DAOSYS010000109 DAOSYS010000000
VERSION     DAOSYS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4892_length_11326_cov_5.463845"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLU99_08250"
     CDS             complement(<1..800)
                     /locus_tag="PLU99_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOO17087.1"
                     /translation="MSAKSRRHHGRRARDLARRPKPGTPPGTLHPPPEASPSEVSVLA
                     FGPAGHEEVRLPARADLTRLPALLEKWPVTWVNVEGLGDAEVLAELGRIFNLHHLALE
                     DVLHPHQRAKAEPYGESYFIVARMPVREQPWRTEQVSIFLGPRYVLTFQEGPRGDPLD
                     PIRERIRSGALGNRARPDYLVHAILDAIVDHYFPLLEQCGERLDALEEMEAGVTRPVP
                     ALMSGVHAVKRDLLTIRRIVWPLRDALNALLRDPTRLITDETRMYLRDL"
     gene            1031..3457
                     /locus_tag="PLU99_08255"
     CDS             1031..3457
                     /locus_tag="PLU99_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17088.1"
                     /translation="MNVTDQFEKLGAFYLGREHDLQTRQRGETPVLYDSRDLVTHAVC
                     VGMTGSGKTGLCIDILEEAAIDGIPAIVIDPKGDLTNLLLTFPELRPEDFAPWINEDD
                     ARRAAVTPAQFAEQQAALWARGLAEWGQGGARIQKLMDAAQFSIYTPGSGAGLPVSLL
                     RSFAAPPRTLVEEPELFRERISGTVAGLLGLIGVQADPQSREHILLSTILAQGWAAGH
                     DVDLPNLIQQIQQPPVRRVGVMDLDTFYPPKERFELALQLNALLAAPGFTRWTEGEPL
                     DIAALLHTPEGHPRVSIFSIAHLSDAERMFFVALLLNETLAWVRTQSGTTSLRAILYM
                     DEIAGYCPPVANPPSKPPLLMLMKQARAFGLGLVLATQNPVDLDYKGLSNAGTWFIGR
                     LQTERDKARVLDGLEGAAAGAAAKFDRATVDRMLSQLGNRVFLMNNVHEEGPRVFETR
                     WALSYLRGPLTREQIRLLMEPRKREAAFSQPAPTSDSAGAPPAISTPTAGGDANVTST
                     PPALPPDIPQYFIPARQTGRILYKPMLFGCANVHFRDSKAGVEHDTAACYVAPLGSGP
                     GGVDWDAAVQSDFSDTDLKTQPEPGGSFAALPSEATRAAFFKRCTGSFADMVFRTASL
                     KLLRSPQLKQVSKVGESERDFRLRLQQSAREQRDEQVEKLRNKYAPKVAALRDRISRA
                     EQAVQVQKSQASAAKLQTAVSAGAALLGALTGRKVASAGNVGRVATAARGAGRAAREA
                     GDVERAEQRVAVLERQLHELQAQFEREVEKLAARLNPQTERLEEVTVRPRKADVRVRA
                     VVLAWAPLPQ"
     gene            3680..4102
                     /locus_tag="PLU99_08260"
     CDS             3680..4102
                     /locus_tag="PLU99_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HOO17089.1"
                     /translation="MPAKKTTERARKDKQQGKSASTQAGEFVREEIHHVRQGKHGARS
                     AKQAIAIGLSKARRAGVELPAPKKGQTSESTRKKAQRDVQRGQGKSKTGENGVSRKRS
                     QAGQKALKREGQKAASHTALSRQAKQAARRRRQPAASR"
     gene            complement(4179..4850)
                     /locus_tag="PLU99_08265"
     CDS             complement(4179..4850)
                     /locus_tag="PLU99_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOO17090.1"
                     /translation="MTTAVYDDITVEVPAGRRQLEASLTVPPDAKGIVLFAHGSGSSR
                     HSPRNRFVAQHLNNERLATLLLDLLTPAEEEVDARTAHLRFNIDLLAARLGQASDWVH
                     GNPDLRELPIAYFGASTGAAAALVAAADRPDLVRAIVSRGGRPDLALPVLGRVTAPVL
                     LIVGENDAPVIRLNRMALQEMLCYRELEIVPRATHLFEEPGALEQVARLAAQWFGRFL
                     AQAGA"
     gene            complement(4869..5540)
                     /locus_tag="PLU99_08270"
     CDS             complement(4869..5540)
                     /locus_tag="PLU99_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOO17091.1"
                     /translation="MSAAPLFRDRTDAGRALAARLTHYADQPDVLVLALPRGGLPVGF
                     EVATALRVPLDVFVVRKLGLPGHEELAMGAVASGGVRVLNRELVDYVGVPRDVIDHLT
                     AMAQRELRRREQLYRGDRPPPDVRGRRVILVDDGLATGATMRAGMEALRQADCARIVV
                     AVPVAAADTCEDLARLADEVVCLATPEPFFGVGMWYEDFSQLTDDQVRELLARERELH
                     SLANG"
     gene            complement(5626..6555)
                     /locus_tag="PLU99_08275"
     CDS             complement(5626..6555)
                     /locus_tag="PLU99_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO17092.1"
                     /translation="MHGPAMGTPAPFRIAHLSDMHLTARPDQRRTGCDVFKPLTGMNA
                     ALGTILQSPLVCTCDLILVTGDITDNGAPEAWTHFWETAREAGVADKIRALPGNHDVC
                     WLGPARFPRRGLKQADLLKAVRGLQLGGQATKFPWVYLPDPRIAIFGLNSNNLGNLSV
                     GTNAMGDLGYYQLKRLAALLYRHREVPIKIVALHHSPNIPEMETARRRKQRPMSRLER
                     LAHQIPREQRRALLLLCIAHNVRLVAHGHLHVEEDRRITGTRIVGAPASTEPSNAGHY
                     HFWRYTIRRSEPRLYITRHSVAVAPPPIVLKAR"
     gene            complement(6653..8059)
                     /locus_tag="PLU99_08280"
     CDS             complement(6653..8059)
                     /locus_tag="PLU99_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOO17093.1"
                     /translation="MRIEINSRLKRRGIWRFSVFPVLMACIAPPATAGPPTLQAEWSW
                     TPSQGIEWVEQALPGRSELLVNTRDGSLHVFNLESGKALLTEPIPARPGLRPVPVPLI
                     PQSLDPYSQTYCFDRFTVLAVRLMPQPGLAWRVGTWCEGLAEPAPPGGADPNKTFQGD
                     PEELCQLLAAAHTPWGVIVARNDGRFGLLDRADGRVVWQHKFEAIALARLHVWGSEAA
                     LLWRSGPEVHAISMDVRTGRFREYPILPERPWPFWSSLTGGGLVLIEPERCIFATPRS
                     AQTVFSTPAGTNIIAASVALQPESPTAETSTGPVVIFGTSDGVLRAINPQDGREYWTA
                     THGPAMQATGGAVGQSWTWVKLLGDLVVSAAGETAQVRAASTGDLLAEFKAETPVRLL
                     DAAVIAGRVWILLEHAAGQPRFELVSVNQVTQTAPAGDTETPRFRLDQPGIYLGALWA
                     DRHLVVSTRSGIAAFVIP"
     gene            complement(8171..9271)
                     /gene="wecB"
                     /locus_tag="PLU99_08285"
     CDS             complement(8171..9271)
                     /gene="wecB"
                     /locus_tag="PLU99_08285"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOO17094.1"
                     /translation="MAKIINICGARPNFMKIAPLMRAYAKHPNLEPMLVHTGQHYDDN
                     MSDLFFRQLQIPKPDVNLEVGSGSHARQTAEIMTRFEPLVLEHRPEWVVVVGDVNSTI
                     ACGLVATKLGVKVAHVEAGLRSFDRSMPEEINRLLTDAISDLLFVSEPSGLENLRKEG
                     VDEGKIRFVGNVMIDTLRFNLERAAQSTILQTLKLQPGGYNVLTLHRPSNVDDPTVFG
                     RIAAALEEISADLPTVFPIHPRTVHNLEKLGFSERFKRMSGLRIIEPLGYLDFLHLMG
                     KAAVVLTDSGGIQEETTILGVWCLTVRENTERPVTLNQGTNTLVGTDPQRIVSEYRRC
                     RSQPAGTRPIPEKWDGKAAERIAAILAEQCSA"
     gene            complement(9280..9891)
                     /gene="rsmD"
                     /locus_tag="PLU99_08290"
     CDS             complement(9280..9891)
                     /gene="rsmD"
                     /locus_tag="PLU99_08290"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOO17095.1"
                     /translation="MRIIAGSFRGRKLEAPPGTATRPITDRAKETLFNILGARLGLPG
                     QLPDVEVLDLFAGTGGLGLEALSRGAKSCLFVERDRRALAALRRNIETLHAQAACRVS
                     IENAWTMRVPPAAGGYGLVFVDPPYKDALAPLPVIDLLERLAPQLAPGGLIVFRHSHR
                     SRLSLEGLRTLECVDQREVGTMRLWLFAARAAGPETPPPDSPT"
     gene            10088..10873
                     /locus_tag="PLU99_08295"
     CDS             10088..10873
                     /locus_tag="PLU99_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOO17096.1"
                     /translation="MIRDLRTIIDGWEYEPGKISVRKIIGRDGREKIQTRIDMGLLQM
                     EPDGPPDGQRIAGYDSVLERLEAELRRHIAVYGDDQDFVLSPEQCVDLRREAYLYYQR
                     YLSLFVLEDYDRVARDTARNLRAIELCMKYAASAEDRSNLAAQRGYVLMMHARSRACA
                     ALREGRYDDALRLVTEGTAALQAANEEALDEQASLASEIRVLAALREEIMSKMPPDSP
                     ARLRHELQEALEGEDYERAAVLRDRLAVSKLAVSKKEQKASLP"
     gene            11004..>11326
                     /locus_tag="PLU99_08300"
     CDS             11004..>11326
                     /locus_tag="PLU99_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO17097.1"
                     /translation="MANEASLAWQIALRAHTEGAQLAFNHFPGPKMERRVRQLAEPIG
                     ARLIVPCDVNDDAQIAAAFHQAGETLGPLDFLVHSVAYASHAALGGPFFQTNRADFLQ
                     SMEISV"
BASE COUNT         2077 a         3611 c         3684 g         1954 t
ORIGIN      
        1 agatcgcgca ggtacatacg ggtttcgtcc gtgatcagcc gggtcgggtc gcgcagcagg
       61 gcgttcaacg cgtcccgcag gggccagaca atgcggcgga tcgtcagcaa gtcgcgtttg
      121 accgcgtgta caccggacat cagcgccggc accggtcgcg tcacgccggc ctccatttcc
      181 tccagcgcat ccagccgttc gccgcattgc tccaacagtg gaaaatagtg gtccacgatg
      241 gcgtcgagaa tggcgtgcac gaggtagtcc gggcgggcgc ggtttcccag ggcgccactg
      301 cgaatccgtt cgcggatcgg gtcgagcggg tcgccgcgcg gcccctcttg aaacgtgagc
      361 acgtaacgcg ggccgagaaa gatgctgact tgctcggtac gccacggctg ctcgcgaact
      421 ggcatgcgcg ccacaatgaa gtagctctcg ccgtacggct cggctttggc gcgctggtgc
      481 gggtgcagca cgtcctccaa cgcgagatgg tgcaagttga agatgcggcc caattcagcc
      541 agtacctctg cgtcacccag tccctcgaca ttgacccaag taacgggcca tttttcgagc
      601 agggcgggca gccgggtcag gtcagctcgg gccggcagcc ggacttcctc gtggccagcc
      661 ggcccaaagg ccaacacgct gacttcgctg ggcgaggcct cgggtggcgg gtggagcgtt
      721 ccggggggag tgccaggctt cgggcggcgg gccaggtcgc gtgcccggcg gccgtggtgg
      781 cgtctgcttt tggcggacat gtttgtcctc cgcggcacag gcgaacgttc gatcttaacg
      841 cggatttcgg gtggtagacg ccgccggtcc aagcggtgga aaggaggtga gaacgtgtcc
      901 gccttcggct ggccgcataa cctctttgca ggatcgcagg gcggccatgg cccggaaccc
      961 ggcaccggat agggatttcc gccgccgagt atgcgactag taaaacgaga gtaaaccccg
     1021 gcgaaatgta atgaacgtaa cagatcaatt cgagaagctc ggggctttct acctcggccg
     1081 ggagcacgac cttcagacgc gtcagcgcgg cgaaacgccc gtactttacg actcgcggga
     1141 cctggtcaca catgccgtgt gcgtgggcat gactggcagc ggaaagaccg ggctgtgcat
     1201 cgacatcctg gaggaggcgg cgatcgacgg cattccggca attgtgatcg accctaaagg
     1261 cgacttgacc aacctgttac tcaccttccc cgagctgcgc ccggaagatt tcgcgccgtg
     1321 gatcaacgag gacgatgcgc ggcgtgcggc tgtgacgccg gcgcaatttg cggagcagca
     1381 ggcggcgctc tgggcgcgcg gactggccga atggggacag ggcggagcgc gaattcagaa
     1441 gctcatggat gccgcgcaat tctcgattta cacgcccggt agcggcgccg gcctgcccgt
     1501 ttcgctgctg cggagcttcg ccgctccacc gcggaccctg gtcgaggagc cggagttgtt
     1561 tcgcgagcgc atctccggca cggttgccgg cctgctggga ttgatcggcg tgcaggctga
     1621 tccgcagagc cgcgagcaca ttttgctttc gaccatcctc gcgcagggct gggccgcggg
     1681 gcacgacgtg gacctgccga acttgatcca gcagattcaa cagccgccgg tgcgccgcgt
     1741 cggcgtgatg gacctggaca cgttctatcc gccgaaggag cgcttcgagc tggcgctgca
     1801 actgaatgcg ctgctggcgg cgccgggctt cacgcgctgg actgaggggg aaccgttgga
     1861 catcgctgcg ctgctgcaca cgccggaagg gcatccgcga gtttcgattt tttcaatcgc
     1921 gcacttgtcc gacgcggaac gcatgttctt cgtcgcgttg ctgctgaacg aaacgctggc
     1981 atgggtgcgg acgcaaagcg gcacgaccag cctgcgcgcc atcctctaca tggacgaaat
     2041 cgccggctac tgtccgccag tggccaatcc gcccagtaag ccgccgctgc tgatgttgat
     2101 gaagcaggcc cgggccttcg gcctgggtct ggtgctggcc acgcagaacc cggtagacct
     2161 ggactacaag gggctttcga atgccggcac gtggttcatc gggcgccttc aaacggagcg
     2221 cgacaaggcc cgggtgcttg atgggctgga aggggcggcc gccggcgcgg ctgccaagtt
     2281 cgaccgcgcc acggttgacc gaatgctctc gcaattgggc aatcgcgtgt tcctgatgaa
     2341 caacgtccac gaggaggggc cgcgcgtgtt cgagacgcgc tgggcgctga gctatctgcg
     2401 cgggccgctc acgagagagc aaatcaggct actgatggaa ccgaggaaac gcgaagcggc
     2461 cttctcgcag cccgccccga cttcggattc agccggtgcg ccgccggcca tatccacgcc
     2521 gaccgctggc ggagacgcga atgtgacttc gaccccgccc gcgctgccgc cggacatccc
     2581 gcagtatttc attccggccc ggcagacggg aagaattctc tacaagccga tgctcttcgg
     2641 ctgtgcgaat gtccacttcc gcgattccaa ggctggcgtg gagcacgaca ccgcggcttg
     2701 ttacgtggcc ccgctcggct cggggcccgg cggtgtggac tgggacgcgg cggtgcagag
     2761 cgacttctcg gacacggatc tcaagactca gcccgagccc ggcgggtcgt tcgccgcact
     2821 gccgtctgag gcgacgcgag ccgcattttt caaacgttgc accggcagtt ttgcggacat
     2881 ggtgttccgc acggcgagct tgaagctcct gcgcagcccg cagcttaagc aggtatcgaa
     2941 agtaggggag tccgagcgcg atttccggct gcggcttcag cagtccgccc gcgagcagcg
     3001 cgacgagcag gtggagaagt tacggaacaa gtacgcaccg aaggtggccg cgctgcgcga
     3061 caggatcagc cgcgcagaac aggccgtcca agttcaaaaa agccaggcct cagcggccaa
     3121 gctgcagacg gccgtctcag ccggtgccgc gctcctgggg gcattgaccg ggcggaaggt
     3181 agccagcgcc ggcaacgtcg gccgcgtggc aaccgcagct cgcggcgcgg gccgtgcggc
     3241 acgcgaagcc ggcgacgtcg aacgggccga gcagcgagtc gcggtcctgg agcggcaatt
     3301 gcacgagctg caggcgcagt ttgaaagaga agtggaaaaa ctggccgcgc ggctcaatcc
     3361 gcagacggag cgactcgagg aagtcaccgt caggccgcgc aaggcggacg tgcgcgtacg
     3421 cgcggtagtc ctggcatggg cgcccctccc gcaatgacgt gataaggtga taaagtgata
     3481 gggtgataaa ggacggtccg cgggttccct ttatcaacct atcactttat cactttatca
     3541 cctttccatg agtgcgttct caccttgaat tcatcgcgcg ttcaggtggc gccgcaaact
     3601 aaacaggtgg ttggcgcggt accgtcgcgt agggcggccg ggggcgcgcc gtcgccgttc
     3661 gaatgccagg agacgattcg tgcctgcaaa gaaaaccaca gaacgcgccc gcaaagacaa
     3721 gcagcagggc aagtctgcca gcacccaggc cggggagttc gtgcgcgagg aaatccacca
     3781 cgtgcgccag ggcaagcacg gggcccgcag cgccaagcag gcgattgcga tcggcctctc
     3841 gaaagcgcgc cgcgccggcg tggaactccc ggctcccaag aagggccaga cctcagaaag
     3901 cacgcgcaag aaggcgcagc gcgacgtcca gcgcggccag ggcaagagca agaccggcga
     3961 aaacggcgtg tcgcgcaagc gctctcaagc cgggcagaaa gcgctcaaac gcgaaggcca
     4021 gaaggctgct tcccacacgg cgctgtcgcg ccaggcgaag caggcggccc gtcgccgccg
     4081 acagcccgcc gcctcacgct agcgcgccgg tcagcgtagc cctgaattgc ggggtggcgt
     4141 tttgcgggcc agcggtgcgg ccggcacgcc ggacgcccct atgcgccggc ctgcgcgaga
     4201 aatcgcccga accactgcgc cgcgagccgc gctacctgct ccagcgcgcc cggctcttcg
     4261 aacagatgag tcgctctggg cacgatttcc aactcccggt aacagagcat ttcctgcagc
     4321 gccatgcggt tcaagcggat caccggagcg tcgttctctc ccacaatgag cagcacgggg
     4381 gccgtgacac gccccagaac cggaagcgcc aagtccggcc gtccgccgcg cgagacgatg
     4441 gcccgcacca ggtccggccg gtccgcggcc gcgaccaggg ccgcagcagc accggtgctg
     4501 gcgccgaagt aggcgatcgg cagctcccgc aaatcgggat tgccgtggac ccaatcactg
     4561 gcttggccca gccgcgccgc caacagatcg atattgaacc gcagatgcgc ggtccgggcg
     4621 tcgacctcct cttccgccgg tgtcaacaaa tccaaaagca gcgtcgccag ccgctcgttg
     4681 ttcagatgtt gagccacgaa gcggtttcgc gggctatggc ggctgctgcc gctgccgtgc
     4741 gcgaacagca cgatgccttt cgcgtcgggg gggacggtta ggctggcctc gagctggcgg
     4801 cggccggccg ggacctcgac agtaatgtcg tcataaaccg ctgttgtcat ctgagcgccc
     4861 taatcaactc aaccatttgc gaggctgtgg agctcgcgct cccgggccag cagctcgcgc
     4921 acctggtcgt ccgtcagttg cgagaaatcc tcgtaccaca ttcccacccc aaagaatggc
     4981 tccggagtcg cgaggcagac cacttcgtcc gccagccgcg cgagatcttc acacgtgtcg
     5041 gccgcagcta ccggcaccgc aaccacgatg cgtgcgcagt cagcttggcg caacgcctcc
     5101 ataccggctc gcatcgtcgc ccccgtcgcg agtccgtcat caaccaggat cacccggcgg
     5161 ccgcgcacgt ccggcggcgg gcggtcgccg cgatagagct gttcgcggcg gcgcagctca
     5221 cgctgcgcca tagccgtcaa gtgatcgatg acgtcgcgtg ggaccccgac ataatcaacc
     5281 agctcccggt taagaacgcg cacacccccg gaagccacgg cccccatggc cagttcctca
     5341 tggcccggca aacccagctt gcgaacgacg aacacgtcca gcggaacgcg cagtgcagtc
     5401 gccacctcga agcccaccgg cagcccgccg cgcggcaacg ccagcacgag cacgtccggc
     5461 tggtcggcgt aatgtgtgag gcgggccgcc aacgcgcggc cggcatcggt tcgatcgcga
     5521 aagagaggtg cggcggacat tcgttcaccg gcgcgatttt attcgctggt gtgcctgtct
     5581 ccgtactgga gaaccgcggt cgcaacttta attcgatttc agcagttaac gtgctttaag
     5641 cacgatgggt ggcggcgcca ccgcgaccga atgccgggtt atgtagagcc ggggctcgct
     5701 ccggcgtatc gtgtaacgcc agaagtggta atgcccggcg ttcgatggct cagtgctggc
     5761 gggcgcgccc acgatgcgcg tgccggtgat cctgcgatcc tcctccacgt gcaggtggcc
     5821 gtgagcaaca aggcgcacgt tgtgggcgat gcaaagcaat agcaaagcgc gccgctgttc
     5881 acgcggaatc tggtgtgcca gtctttccag ccgtgacatt gggcgctgct tgcggcgtct
     5941 tgcggtctcc atctcaggga tgttcggact atggtgcaga gcgacgattt tgatgggaac
     6001 ttcacgatgg cggtagagca gggccgccag tcgcttgagc tggtaatagc ccaagtctcc
     6061 catggcgttg gtgccgacag acagattgcc caggttattt gaattcaggc caaaaatcgc
     6121 gatgcgtggg tcgggaagat agacccatgg gaacttggta gcttgcccgc ccaattgcag
     6181 accacggacg gctttcagca aatcggcttg tttgagcccg cgccggggaa agcgggccgg
     6241 tcccagccag cacacgtcat gattgccggg cagggcgcgg attttgtcgg cgacgcccgc
     6301 ttcccgcgcc gtctcccaaa agtgcgtcca tgcctcgggc gcgccgttgt ccgtgatgtc
     6361 gccggtgacc aggatcaggt cgcacgtaca caccagcggc gactgcaaga tcgtgccaag
     6421 agcggcattc ataccggtca gcggcttaaa aacgtcgcac ccggtccgcc gctggtcggg
     6481 gcgcgccgta aggtgcatgt cggaaagatg ggcaattcga aatggagctg gtgtacccat
     6541 ggccggaccg tgcatttcga gctccctgcg agctgactgc ttacctgggc agtcgcgcgc
     6601 aggctaactg agtatgcacg gcctcacaag ctcctcgggc gtgtttacag gatcatggga
     6661 taacgaacgc ggcaatgccg cttcgtgtgc tcacaaccag gtgtcggtcc gcccacagcg
     6721 cccccaggta aatgcccggc tgatcgagtc ggaagcgcgg cgtttccgta tcgccggccg
     6781 gcgcggtctg ggtaacctgg tttacgctga ccagctcgaa tctcggctgc ccagcggcat
     6841 gttcgagcag aatccagacg cgaccggcaa tcaccgcggc gtccagcagg cgtaccggcg
     6901 tctcggcctt gaactctgcc agcagatctc ctgtactggc cgcacgcacc tgcgccgttt
     6961 cacccgccgc gctgaccacc aggtcgccca gcagcttgac ccaggtccac gactggccga
     7021 ccgcgccgcc ggtggcctgc atggctggcc catgcgttgc agtccagtac tcccggccgt
     7081 cctgcggatt gatggcacgt agtacgccgt cgctggtacc gaaaatcact accggcccag
     7141 ttgaggtttc cgccgtgggc gattccggtt gcagggccac gctggccgcg atgatgttcg
     7201 tacctgctgg cgtgctgaag acagtctgcg cactgcgcgg cgtggcaaag atgcagcgct
     7261 cgggctcgat cagcacgaga ccgccaccgg tcaggctgct ccagaatggc caagggcgct
     7321 cgggcaaaat ggggtattcg cggaatcggc cagtgcgcac gtccatcgaa attgcgtgca
     7381 cttccggccc gctgcgccag agcagcgccg cctcagaacc ccacacatgc aggcgcgcca
     7441 gcgctatcgc ctcgaatttg tgctgccata ccacgcggcc gtcggcccgg tccagcagac
     7501 cgaagcgccc gtcgtttcga gctacgatga cgccccaggg cgtgtgtgcg gctgcgagca
     7561 gttgacacag ctcctcggga tcgccctgaa aagtcttgtt cggatcggcg ccgccaggcg
     7621 gagcgggttc ggccagccct tcgcaccagg taccgacacg ccacgcaagg cccggctgcg
     7681 gcatcaagcg gactgccaga accgtgaaac ggtcaaagca gtaggtctgc gagtagggat
     7741 caagagattg aggaatcaag ggaacgggaa ccgggcgaag tccggggcgc gcgggaatcg
     7801 gctcagtgag cagtgctttg cccgattcaa gattgaagac gtgcaggctg ccgtcgcgcg
     7861 tgttgaccag caattcggag cggccgggaa gcgcttgctc gacccactca ataccttgcg
     7921 agggggtcca gctccattcg gcctgcagtg tgggcgggcc ggccgtcgca ggcggcgcga
     7981 tgcacgccat caggaccgga aagactgaga accgccaaat cccgcggcgt tttagtcgac
     8041 tgttgatttc gatgcgcatc ttcgcctacc ggttgcccaa ttgtatgcga gatgctggaa
     8101 gccgcgagtg acgggcggtt ctagatcact tatcgccaaa tggccaaacc gccgagtcgc
     8161 taaacaccaa tcacgctgaa cactgctcag ccagtatcgc agcgatccgt tcggccgctt
     8221 tgccgtccca tttttcgggt atcggtcgcg tgccggcggg ttggctgcgg caacgccggt
     8281 attcggacac gatgcgctgc gggtcggtcc cgacgagcgt gtttgttccc tggttcaaag
     8341 tcaccgggcg ttcggtgttc tcgcgcaccg tgagacacca gacccccaga atcgtcgtct
     8401 cctcctggat gccgccggaa tcagtcagca ctactgcggc cttgcccatc aggtgcagga
     8461 agtcgagata gcccagcggc tcgatgatcc gcagcccgct catgcgcttg aaacgctcac
     8521 tgaaacccag cttctccagg ttgtgaaccg ttcgcggatg gatcggaaaa acggtcggca
     8581 aatcggccga aatctcttcc aaagccgcgg cgatgcggcc gaaaaccgtc gggtcatcga
     8641 cattgctcgg ccggtgcagc gtaagaacgt tgtagccgcc gggctggagc ttcaacgtct
     8701 gcaaaatggt cgactgggcg gcccgttcga ggttgaagcg cagggtgtcg atcatcacgt
     8761 ttccgacgaa gcgaatcttg ccttcgtcga cgccctcttt gcggagattt tcgaggccgc
     8821 tgggctcgga gacgaaaagc aggtctgaaa tcgcatcggt cagcaaccgg ttgatttctt
     8881 ccggcatact gcgatcgaag ctgcgcagcc cggcctcgac gtgggccact ttcacgccga
     8941 gcttggtggc caccagcccg cacgcgatgg tgctgttcac gtcgcctacg acgaccaccc
     9001 attccggccg atgctcgagc acgagtggct cgaagcgggt catgatttcg gccgtctgtc
     9061 gggcgtgact tccggacccg acttccaggt tcacgtcggg cttgggtatt tgcagttgtc
     9121 ggaagaaaag gtccgacatg ttgtcgtcat agtgttgccc ggtgtgtacg agcatgggtt
     9181 ccaggttggg atgctttgcg tacgcccgca tcagcggcgc gattttcatg aagtttggcc
     9241 gcgctccgca gatgttgatg atcttcgcca ttgcgaacct caagttggag aatctggcgg
     9301 cggcgtttcc ggcccggccg cccgcgccgc aaacagccac agccgcattg ttccgacctc
     9361 gcgctgatcc acgcactcca gcgtgcgcag cccttccagc gacaaccgtg agcggtggct
     9421 gtgccggaag acgatcaacc cgcctggggc aagctgcggc gccaggcgtt caagcaaatc
     9481 aatcaccggc aggggggcca gggcgtcctt gtagggcgga tcgacgaata ccagaccata
     9541 gccgccggcg gcagggggca cgcgcatcgt ccatgcgttt tctatcgaca cgcggcaagc
     9601 cgcctgtgcg tgaagagttt cgatattgcg gcgcagcgcg gcgagggccc gccggtcgcg
     9661 ttccacaaag aggcacgatt tcgcgccgcg cgacagagcc tccaagccga gcccgccggt
     9721 gccggcaaac aagtctagca cctccacatc ggggagttgg cccggcaggc ccaggcgggc
     9781 gccgagaata ttgaaaagcg tctctttagc gcggtcggta atcgggcgtg tcgccgtgcc
     9841 cggcggcgcc tccagtttcc gacctcgaaa gcttcccgcg attatccgca taccggaacg
     9901 cgactattcg catctcccaa tatcggcgcc gcctgtggcg gcctccgaat cctgtacggc
     9961 ccgcgtgacg gattttgtcg tgctccggct ttccacacgc gccggctccg ctcaccttgt
    10021 taccggctca tcacgttgtg agcggctcat tatgacccgc ccatccgcag ccgatacgca
    10081 tactggcatg atacgcgacc tgcgaaccat catcgacggc tgggaatacg agcccgggaa
    10141 aatctcggtt cgcaaaatta tcggccggga tgggcgcgag aaaattcaga cccgcatcga
    10201 catgggtctg ctgcagatgg agccggacgg cccgcccgac ggccagcgga tcgccggcta
    10261 cgattctgtc ctcgagcgtc tcgaggcgga gctgcggcgt cacatagccg tttacggcga
    10321 cgaccaggac ttcgtgctct cgccggagca gtgcgtggac ttgcggcgcg aggcttacct
    10381 ctactaccag cgttacctgt cgctgttcgt gctggaagac tacgaccgcg tggcgcgcga
    10441 cacggcccgc aacctgcggg caatcgaact gtgcatgaag tatgccgcgt cggccgagga
    10501 ccgctccaac ctggcggcgc agcgcggcta cgtactgatg atgcatgcac gttcgcgtgc
    10561 ctgcgcagcc ctgcgggagg gacgctacga cgacgccctg cgcctggtga cggaagggac
    10621 cgccgcgctg caggccgcga acgaagaggc gctggacgag caggcttcgt tggcgtccga
    10681 aatacgggtg ctggcggcgc tgcgcgagga aatcatgtcc aagatgccgc ccgactcgcc
    10741 cgcccgcctc cgccacgagc tgcaggaagc gttggagggc gaggactatg agcgcgctgc
    10801 cgttctaaga gaccgcctgg ccgtgtccaa attggccgtg tccaaaaaag aacagaaagc
    10861 ttccttgcct taattactcc gtcctccggg cccacgcgcc ctgtccaaag cgtccatgtt
    10921 gatgcgctcc ggaaaaacgc ccactggggt tatacaattc tgcaatgggt ttaatggacg
    10981 ggaaaagagg ttttgtcttc ggcgtggcga acgaggcttc gctcgcgtgg cagattgcgc
    11041 tgcgcgccca caccgagggc gcgcaattgg cgttcaacca tttccctggc ccgaagatgg
    11101 aacgccgcgt gcgccagttg gccgaaccga tcggggcgcg gctgatcgtg ccgtgcgacg
    11161 tgaatgacga cgcacagatc gcggcagctt tccaccaggc gggcgagacg ctgggcccgc
    11221 tggattttct ggtccattcg gtcgcctatg ccagtcacgc cgcgcttggc ggccccttct
    11281 tccagacaaa tcgggccgac tttctccagt cgatggagat tagcgt
//
LOCUS       DAOSYS010000110        11258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4928_length_11258_cov_5.622155, whole genome shotgun sequence.
ACCESSION   DAOSYS010000110 DAOSYS010000000
VERSION     DAOSYS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4928_length_11258_cov_5.622155"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..930)
                     /locus_tag="PLU99_08305"
     CDS             complement(283..930)
                     /locus_tag="PLU99_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOO17098.1"
                     /translation="MHFEWDDLLRLLFACAAGALIGMERELRGKPAGLRTNVLICVGA
                     ALFTLISVHVAEGSTTHDPGRIAAQVVTGVGFIGAGAILHARGSVFGLTTAAGIWLVA
                     SIGMAFGAGALVLGGVATLLSLGVLMGLGFAESRVARWRTTAKFQIKMEPSPELSRAI
                     KQHLRELDLPRRTWRVSKVHGAYTGYLVTVGPEQRLEELQKRIMAEPGVISLVRH"
     gene            1232..1305
                     /locus_tag="PLU99_08310"
     tRNA            1232..1305
                     /locus_tag="PLU99_08310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Arg,seq:ccg)
     gene            1538..2137
                     /locus_tag="PLU99_08315"
     CDS             1538..2137
                     /locus_tag="PLU99_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17099.1"
                     /translation="MKISITASWRYRLALPVLLALVGCCGTTKRVDPDALSDYEVGTG
                     LTSQDFRSVCQRMARSLLNVPEIQNACEPPMVAIAPVVNDSNDYIDGTEFARKIRTEL
                     IKFAEGRVRFLDRELTRPIEAENRDKRRGKITGEEATRHGADFFLTGRIAAIDRVVGG
                     GATTYYRLSFRLTEAGNSLIVWEDDYEIKKQSTAASVYR"
     gene            2148..3572
                     /locus_tag="PLU99_08320"
     CDS             2148..3572
                     /locus_tag="PLU99_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17100.1"
                     /translation="MSSTRARRRRVCNCFALGLLSTLPIALAGCASPDDRLARALAIR
                     HSVDPDYRSAATVLESLQNDPRCKAEYVLYGMCGTVVNVCGGDYRDAERVGKDTYIAV
                     KKYQDASAETRAAWGRESIKFFKGEPHERSMLAWYLGLAAYLRGEYNDARIFFTQSLL
                     AAATRDEDCAQFRDDFQLGHYWLGRTYQHLGQLDNARVAFEKAGCVLAHRGQDRELAR
                     LKKQRAGEYRRELKLEAACYRTATKGKQPVAGAIDLSAERARSDLPELLPDAAEGSPV
                     LASAQDLSSFLDPEFQQEANLIIVIELGTGPLKYLAGSQNERDEVAPGMYKERFVDVY
                     IDGHRVGPALPLCDMYHQACTRGVQTRRGRQTAKMIAKEVLRRVPLAGTVFGYWDVSG
                     DARFVPVLPGEVHVLAARVKPGLHTVALRCSDINGQYLPRFDADYHFLPVAEDEETVL
                     VLQTRENYDNAYLLAQTEAQAATN"
     gene            3595..4038
                     /locus_tag="PLU99_08325"
     CDS             3595..4038
                     /locus_tag="PLU99_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17101.1"
                     /translation="MACAAVVLGLLCGCATPPPAYVPVRRDNIRIVHCFDSVGHQPGS
                     KPWIMGGTCCCTPTPEVMQAYQRDGFCAGMTAEELIEMYHARGIALAIDHMWCNNLCQ
                     EGPHVTQGGRCMVPPTPGTRQFERVVTGQGMVSLAQQTSGPGEEE"
     gene            4043..4465
                     /locus_tag="PLU99_08330"
     CDS             4043..4465
                     /locus_tag="PLU99_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17102.1"
                     /translation="MNSIIRACAVFCGLCLTGCASTHPAEINDCPAPYQAKSRVQWYS
                     SNLKSLLEIDKADVQRTPDGRLHLRLAIRNQTRKDIWVDIRTVFTDDRGFEREKTNWE
                     PICLTARVDTTYEVFSLGTQVCDYQIVIRDPRKLDCIP"
     gene            4480..5604
                     /locus_tag="PLU99_08335"
     CDS             4480..5604
                     /locus_tag="PLU99_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOO17103.1"
                     /translation="MKATFFSLACCAMPLFAAFVVGCASNEPCDQFAPEPPPPPPACE
                     RPAPTFHYPHEARRATDAALRRKLVIGLARFLEEPGLESTTTEAPADPGSGNVTVKQE
                     VHIGGSRVSQAETDQELFWRRMLKRELLDSGTFTLVERDGLLHILNEIRFGESRYVNP
                     ETSPEVGEIIAVQYLLEASIGGTQDLTFKGTVDPLPRYDERSESLAREILATDRGMCL
                     NRLQEARRLRLRAAHANAMLDENPYSVYLSLYSVRTSEIVAEAAGIGKTPLDAVRDAV
                     EELVDACAEIAPPPRIAWIDGQRVFIDLGRDDGVLVGQRLRYMQEGRPIRNAAGQVIG
                     YDTQEGGVLEITRVEPLMSVAHVVERVLDPQIGDPLTAEN"
     gene            complement(5655..7475)
                     /locus_tag="PLU99_08340"
     CDS             complement(5655..7475)
                     /locus_tag="PLU99_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17104.1"
                     /translation="MSSHGRRACSCTGEAPVLQKQLPGANGRPSRRPLQTCAAIGLLA
                     ALILAVHGASLGDGLFMDDYAHFRQLQHSDWSLEGLTNACRLELVGGVLDLWFLPECT
                     LRFFRPLAFGAMKLLYTLGNWDPLVLHAASLGWHLLACTLLMCLLRRLGASHWLAFGV
                     AGLFAVHPASLATIQWIACQTELMVTVMLLGAMLCYLRFRGWSEEEGAAGRARYGWAV
                     GCLVLFAAALGCRENAIMFPLVVLPVELLLWRRRTREMVFFYIMLCAVSAGYLALRAS
                     CLNGAALPPRPYVYTPHDPGFVRYIIDKTCYYLLGEFFTAPIVPFGGEDYLRQRPLMF
                     YGFSAAVVALLAAVGWRFSQRKPGWLALAWLLGFMAPVLPAFASPHHLYLPAAGWAMV
                     AMLLLRGIGGTGLEPRRWRQAIMWAAVLLAGGVFVTLSIFLRLPLSAAQRVEDQVVTE
                     VASQAGQMQDGDTLYMANMPIIAHYVGLAVEHRTGLRDLRVHALTWAPRVLGLVDTDL
                     ESEITWVDDHTVEVRLSGDRYFGGPVGRMVSEASGKTTPTQTDPSAAASGFKVQVLET
                     DAGGVQALRFTFERSLRDPRVHLFWGSPIRWAARLEAPGR"
     gene            complement(7472..8707)
                     /locus_tag="PLU99_08345"
     CDS             complement(7472..8707)
                     /locus_tag="PLU99_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOO17105.1"
                     /translation="MSASPRRRWALTALRIALCLAAVAYLAFNVKWNDYVRLGSKDGP
                     LVRLLGQTDGRFSVLRDGQVIDVPAADIFHEEVKGQRVPDIEYGIRTVMRQMDRWQAF
                     WAIMLFMPVPLMSAVRLVWMMRVQKVRLSLWDSTKLTFAGNFFNFAMPGGMTGGDVIK
                     AYYVTRFTKRKTEAVTTIFLDRVIGLIGLVLMSGAVILVTRDSAHFSHLTLPLGLIVA
                     GLAVSSVVIYSRRIRAALHLRQIVERLPMAGQLQRIGAATLAMRQHKALVIASLLITF
                     GLQGICIVSATIMAWALGMQGAFDYFFIYIAIGFVISAVPISPPQAVGVMESFYVWCF
                     TADGLNTASQAFALAVAVRLIQLVWSVPGVLVPLLGAHLPRRAELLELGEREAAVDPQ
                     LPSPPDGIAPPGVSPASGQ"
     gene            complement(8730..10730)
                     /locus_tag="PLU99_08350"
     CDS             complement(8730..10730)
                     /locus_tag="PLU99_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17106.1"
                     /translation="MAVCWPAVWLVLPVDGAYVAALLLGAAGWGAWPAAWLAAGQSAG
                     RQVCLAMALGLGVLSTAMLALGVAGLLSGPVAWALLAAGGGLGAVRLLRAARGQLGNV
                     DSDSVAGPGLLARTLVLLPLCVPIVVVLYAACLPPGLLWAGEARGYDVLEYHLQVPRE
                     YFDAGRILFLPHNVYASFPQGVETLYLLLMYLAGGPLAAAIPAQLLHAALGGLTVAAL
                     LLWTPRGWPRVLVALVAGTTVWLAYLGSLAYVELGVLFFAAVAAGLLISEKPTLRTAF
                     AAGICAGLAGGCKYTALVLVAGALGSAWLIGMRDGPKTRAVRLGLYVFGAILAFSPWL
                     IRNAAFTGNPVYPFAYGWFGGRDWSAAQAEQWQRGHSLPVESASLGARARIAFDELLN
                     SGMYNQALWLLGLGGLALLATKRAAQGSEPEAPARANVALTGPLRGDTPRFWSVLAIW
                     AALIVIAWAAFTHMPGRFAVPVIIPLALLVGRGVELSAGSFPRPRPALAVFIGFSLAG
                     ALLGDLKLVNMLRDEAASWQSLTGVRLNEMIDRADILRNVNPVNTAVPTGAYVWIVGD
                     ARAFYVTRRCHYTVPFSKDPWLEFSATADPSAAVAWLRTHGVTHVVFSWSESARLRRS
                     YGFPPHVTPAWADALARGGLKLVSSDREQGGAGAFEVYEVMP"
     gene            complement(10979..>11258)
                     /locus_tag="PLU99_08355"
     CDS             complement(10979..>11258)
                     /locus_tag="PLU99_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOO17107.1"
                     /translation="SELVDACTIVTAARPGFEAGDLPDLARLLTHSQIERLRQHVIAS
                     PRIEISATDIRSRVRAGQSVRYLTPEPVASYIARHGLYASAPTGSQRR"
BASE COUNT         2263 a         3792 c         3468 g         1735 t
ORIGIN      
        1 tagagaggat agtcaaaaag agcgcgggtg ttgatctctg gcggtccctg gcggccgctg
       61 cgccggcacg ccagagcaga gccggggcgt gccgccccgg agtaccgcca agaacccccc
      121 aagccgcgca ccccgatcga gcgcttgccg cttcaatgcc gcgcgtgcga gaatccgtct
      181 cgcacgccct ccgccggcta tgccctctcg cctccccgtg ccgggcgcgc cgttaggccg
      241 cgttcaccag gccagccgca ccccgattgc gcacctcccg cttcaatgcc gcacgagcga
      301 gatcacgccc ggctcagcca taattctctt ctgcagctcc tccagccgct gctccggccc
      361 gaccgtcacc aggtagcccg tatacgcgcc gtgaaccttg ctgacccgcc aagtgcggcg
      421 cggcaggtca agttcgcgca ggtgctgctt aatggcccgg ctcaactccg gcgacggctc
      481 catcttgatc tgaaacttcg ccgtcgtccg ccaacgtgcg acgcgcgatt cggcaaaccc
      541 cagccccatc agcaccccca gactgagcag cgtggccacg ccgcccaaga ccagcgcccc
      601 cgcaccgaac gccattccga tgctggcgac cagccagatg ccggccgccg tcgtcaggcc
      661 aaacacggag ccgcgcgcgt gcagaattgc gcctgcgccg atgaagccca cccccgtgac
      721 cacctgggcc gcgatgcgcc ccggatcgtg cgtggtgctg ccctcggcca cgtgcacgga
      781 aatcagcgta aacagcgccg cccccacaca gatcagcacg ttggtgcgta gaccggccgg
      841 tttgccccgc agctcgcgct ccatcccgat cagggctcct gccgcacagg caaacagcag
      901 ccgcagcaaa tcgtcccact caaagtgcat cgttctgcct caaattgacc aggccgcagc
      961 gttctgcgcc gcgcaccagg cccccagaaa tccagggatc gagagccggg gacgcggccg
     1021 gcctggtttc cgatccttcc cgcgaaaaca agaaattcgg ggacatcagg atagattatc
     1081 tcgttcggaa tgatcgaggt cgaggaggcc cgagtgacca gggtcaaggc gacccggtca
     1141 ctccgagaaa gacccgccga ccgcgatgcg gccctgaagc cagcttttac ttgacgctat
     1201 ccgcccgcgg gttagactac gcctgtccca agtcccggta gctcaactgg acagagcgtt
     1261 ggcctccgga gccagaggtt gtgggttcga atcccgcccg ggacgttcgc agccccgcga
     1321 actaagtagc gctcccactt cgcccgcata acccacccta cgcaagtgcc acaaaaaagc
     1381 tccggaacgc taaagatgac ctcttgtgcc cgggttaaca atttgttaat ctggctatgc
     1441 cgttgttttc ttggggcgcc gcgctcagcg gcctcggcaa cggctcggct cggaatcaca
     1501 cgatcgccgc ttcggagcat ggattggagt aggagtaatg aagatcagca taacggcatc
     1561 atggcggtat cggctcgccc tgccggttct cttggctttg gtgggctgct gcggaactac
     1621 caagcgggtg gatcccgacg cgttgtccga ttacgaggtc ggaacagggc tgacgagcca
     1681 ggacttccgg tccgtctgcc agcgtatggc tcgctcactt ctgaatgtgc cggaaatcca
     1741 gaacgcctgc gagccgccga tggttgcaat cgcgcccgtg gtcaacgaca gcaacgacta
     1801 catcgatggc acagagttcg cccgcaaaat ccgcacggag ctgatcaagt tcgccgaagg
     1861 ccgcgtgcgc ttcctggatc gcgagctgac ccggccgatt gaggcggaga accgggataa
     1921 acgccgtggc aagatcaccg gggaggaagc gacgcgtcat ggcgcggatt tcttcctgac
     1981 ggggcggatt gcggccatcg atcgcgtcgt cggtgggggg gccactactt attatcgcct
     2041 cagcttccgc ctgacggagg cgggcaactc gctaatcgtc tgggaagacg attacgagat
     2101 caagaagcag tcgactgccg cgagcgtata ccgctaagga gagccgcatg agttcgacga
     2161 gggcacgccg ccggcgcgtc tgcaactgct tcgcgttggg actgctgagt acgttaccaa
     2221 tagctttggc gggctgcgca tcgccagatg acagattggc gcgcgctctg gccatccgcc
     2281 actcggttga ccccgactac cgctcggccg caactgtcct ggagagcctg caaaatgatc
     2341 cgcgctgcaa agctgaatac gtcttgtacg gcatgtgcgg gacggtggtg aacgtttgtg
     2401 gcggcgacta ccgcgatgcc gaacgagtcg gcaaggacac gtatatagcg gtgaagaagt
     2461 accaggatgc gtccgccgaa acacgagccg cgtggggccg cgagtcgatt aagtttttta
     2521 agggcgagcc gcacgagcgc agcatgctgg cctggtacct gggactggcg gcctacctgc
     2581 gcggcgagta caacgacgca cgcatcttct ttacacagtc gctcctggca gcagcgacgc
     2641 gcgacgagga ctgcgcccag ttccgcgacg actttcagct cggacactac tggctggggc
     2701 ggacctacca acatctgggc cagcttgaca acgcgcgggt agcgttcgag aaggccggtt
     2761 gtgtcctggc gcaccggggg caggaccgcg agttggcgcg gctgaagaag caacgcgccg
     2821 gcgaatatcg ccgggaactg aagttggagg cggcctgcta tcgcaccgcc acgaagggca
     2881 agcagcccgt cgcgggggcc attgatttgt cggcggagcg cgcccgcagc gatctgcccg
     2941 agcttctgcc cgatgcggcg gagggcagcc cggtcctggc cagcgcgcag gacctgagct
     3001 cgtttctcga tccggaattt cagcaggagg ccaatttaat tattgtgatc gagctgggca
     3061 ctgggccgtt gaaatacctg gcgggctcac agaacgagcg cgacgaggtc gcccccggta
     3121 tgtacaagga gcgcttcgtc gacgtgtaca tcgacggcca tcgcgtcgga ccggcgctgc
     3181 ccctgtgcga catgtaccac caagcctgca cgcgcggcgt gcagacccgc cgcgggcgcc
     3241 aaactgcaaa aatgatcgcc aaggaggtcc tgcggcgcgt gcctttggcg ggcacggtat
     3301 tcggctactg ggacgttagc ggcgacgcgc gcttcgtgcc cgtactgccg ggcgaagtgc
     3361 acgtactcgc agcgcgcgtt aagcccggcc tgcacaccgt ggcactgcgt tgctccgaca
     3421 tcaatggaca atacctgccg cgctttgacg ccgactacca tttcctgcca gttgccgaag
     3481 atgaggaaac tgtactagtg ctgcagacca gggagaacta cgacaatgcg tatttgttgg
     3541 cgcagactga ggcccaggcc gcgacgaact gaacatattg cggcgctaaa ctgcgtggca
     3601 tgtgcggcgg tcgtattggg gctgctgtgc ggttgtgcta ccccgccgcc ggcctatgtt
     3661 cccgtacgaa gagacaacat cagaattgtt cattgcttcg atagcgttgg gcatcagccg
     3721 ggcagcaagc cctggattat gggcgggacc tgctgctgca ccccaacgcc cgaggtgatg
     3781 caggcctatc agcgcgacgg cttctgcgcg ggaatgacgg ccgaagagct gatcgaaatg
     3841 taccatgcgc gaggcatcgc gctggcgatt gaccacatgt ggtgcaacaa tctttgtcag
     3901 gaggggccgc acgtcaccca gggtggacgg tgcatggtgc cgcccacacc cggtacgcgg
     3961 cagttcgaac gtgtagtgac cggacaggga atggtttccc tggctcagca aacatccggg
     4021 cccggagaag aggagtgatg acgtgaacag catcatcagg gcttgcgcag tcttctgcgg
     4081 cttgtgccta acgggatgcg cgagcacgca cccggcggaa attaacgact gtcccgcacc
     4141 ctatcaggca aaatcgcgcg tccagtggta cagcagcaac ctcaaatcgc tgctggaaat
     4201 cgacaaggcc gacgtccagc gcacacccga cgggcggtta cacctgcgtc tggccatccg
     4261 caaccagacg cgcaaagaca tttgggtcga tattcgcacg gtattcaccg acgaccgcgg
     4321 tttcgagcgt gaaaagacaa actgggagcc gatttgcctg accgcgcggg ttgacaccac
     4381 gtatgaggtt ttcagcctcg gtacgcaggt ttgcgactac cagatcgtca ttcgtgatcc
     4441 gcgaaaactc gattgcatac cttgacccgg aggcgagcca tgaaagccac ttttttctcg
     4501 ttggcgtgtt gcgcaatgcc gctattcgcc gcgtttgtgg tgggatgcgc cagcaatgag
     4561 ccttgcgacc agttcgcgcc tgagccgcct ccgccgcccc cagcctgcga gcgtcctgcg
     4621 cccactttcc actacccaca cgaagcgcga cgcgccactg acgccgcctt gcgccgcaag
     4681 ctggtgatcg gattggcgcg cttcctggag gagcccggcc tggagagcac caccactgag
     4741 gcgcccgctg atccgggatc gggaaacgtg acagtcaaac aggaggtgca catcggcggg
     4801 tcgcgcgtga gccaggcaga aaccgaccag gagctcttct ggcgccgcat gttgaaacgc
     4861 gaattgcttg acagcggcac gttcacactg gtcgagcgcg acggactgct ccacattctg
     4921 aacgaaatca ggttcggcga gtcgcgctac gtcaatcccg agaccagtcc tgaagtgggc
     4981 gagatcattg cggtccagta tctactggag gcctccatcg gcggaacgca ggacctcacg
     5041 ttcaagggaa ccgtcgatcc cctgccccgc tacgacgagc gctccgaatc gctggcgcgc
     5101 gaaatcctcg ccaccgatcg cggcatgtgc ctgaaccggc tacaggaagc gcgccgcttg
     5161 cgactgcggg ccgctcacgc caacgccatg ctggatgaaa acccatacag cgtgtacctc
     5221 agcctataca gcgtacggac cagcgaaatc gtggcggagg cggccggaat cggcaaaaca
     5281 cccttggacg ccgtgcgcga cgcggtggag gaattggtcg acgcctgtgc cgagattgcg
     5341 ccgccgccgc gcatcgcctg gatcgacggg caacgcgtat tcatcgacct gggccgcgat
     5401 gacggagtcc tggtcggaca acgcttgcgg tatatgcagg aaggacggcc aatccgaaac
     5461 gcagcgggac aggtgatcgg ctacgacacc caggaaggcg gagtactcga gatcacgcgc
     5521 gtcgaaccgc tgatgtcggt cgcgcacgtg gtcgagcgcg tacttgatcc ccagatcggc
     5581 gaccccctga cggccgaaaa ttaggccgtc acgcctccgt cagcctgacg aaaccagacg
     5641 accgcgcacc gccgtcaccg cccgggcgct tccagccggg ccgcccaacg gatcggtgac
     5701 ccccagaaca gatgcacgcg cgggtcgcgc agcgaccgct caaacgtgaa gcgtagcgct
     5761 tgcactccgc ccgcgtcagt ctccagcacc tggaccttga agcccgatgc ggccgctgag
     5821 gggtcagtct gggtcggagt cgttttgccc gaggcttcgg acaccatgcg gcccaccggt
     5881 ccgccgaagt agcgatcgcc gctcagccgc acctctaccg tgtggtcgtc cacccaggtg
     5941 atttcgctct ccaggtcggt atcgaccagc cccagcacgc gcggagccca cgtcagcgca
     6001 tgcacgcgca gatcgcgcag cccggttcgg tgctcaaccg ccaagccgac gtagtgcgcg
     6061 ataataggca tgttggccat gtaaagcgta tcgccgtcct gcatctgccc cgcctgcgag
     6121 gcgacttcgg tcaccacttg atcctcaacg cgctgcgctg cactcagcgg caggcgcaga
     6181 aagatgctta gtgtgacgaa gactccgccc gccagtaaaa cggcggccca catgatcgct
     6241 tgacgccagc gcctcggctc aagccctgtg ccgccgatgc cgcgcagcag cagcattgcc
     6301 accatcgccc aaccggcagc cggcaagtac aaatggtgcg gcgaggcgaa cgccggcagc
     6361 accggcgcca tgaaccccag tagccacgcc agcgccagcc agccgggctt gcgttgcgaa
     6421 aaccgccaac ccacggccgc cagcaacgcc actacagcgg ccgagaagcc gtaaaacatg
     6481 agcggccgct ggcgcaaata gtcctcgcct ccgaacggca cgattggcgc cgtgaagaat
     6541 tcgccgagca ggtagtagca ggtcttgtcg atgatgtagc gcacgaagcc cggatcgtgc
     6601 ggcgtgtata catatggccg cggcggcagc gccgctccat tcaggcaact ggcgcggagc
     6661 gccaagtagc cggcactgac ggcgcacagc atgatgtaga agaacaccat ttctctggtg
     6721 cgccgccgcc atagcagcag ctcgaccggc agcaccacca gcggaaacat gatcgcgttt
     6781 tcgcggcagc cgagcgccgc ggcgaacaac accaggcagc cgacggccca cccatatcgg
     6841 gcgcggccgg cggcgccttc ttcctctgac caaccgcgga accgcaggta acacagcatg
     6901 gcccccagga gcatgacggt caccatcagc tcagtttggc aggcgatcca ctggatggtc
     6961 gcgagcgatg cagggtgcac ggcgaacaag cccgctacgc caaaggccag ccagtgcgag
     7021 gcgcccagcc ggcgtaagag gcacatcagc agcgtgcacg cgagcaggtg ccagccaagc
     7081 gaggcggcgt gcagcaccag cgggtcccag ttgccgagcg tgtagagcag cttcatcgcc
     7141 ccgaacgcca aggggcggaa gaaacgcagc gtgcactccg gcaaaaacca gaggtcgagc
     7201 acgccgccga ccagctcgag ccggcaggcg ttcgtcagac cctccaacga ccagtcacta
     7261 tgttgaagct gccggaaatg ggcgtaatcg tccatgaaca ggccgtcgcc caacgacgcg
     7321 ccatgaacag cgaggatgag cgccgctaga agcccgatcg cggcacacgt ctgcagtggc
     7381 cgccgcgaag gccggccgtt ggcgccggga agctgcttct gtaagaccgg cgcctcgccc
     7441 gtgcatgaac aagcgcggcg tccgtgcgag ctcattgacc tgacgccggg gacacgcccg
     7501 gcggcgctat tccgtccgga ggcgaaggta actgaggatc caccgcggcc tccctctctc
     7561 cgagctccag cagctcagca cgccgcggca gatgcgcacc cagcagcggc accagcacgc
     7621 ccgggacgga ccagacaagc tggatcagcc gcaccgcaac cgccagggcg aacgcctgcg
     7681 aggccgtgtt cagcccatcc gcggtgaaac accacacgta gaaagactcc atcacgccga
     7741 ccgcctgggg cggcgagatg ggcacggctg agattacgaa gccgatcgca atatagatga
     7801 agaaatagtc aaacgcgccc tgcatgccca gagcccaagc catgatggtc gcgctgacga
     7861 tacaaatgcc ttgaagcccg aacgtgatca gcagcgaggc gatcaccagt gccttgtgct
     7921 gccgcattgc cagcgtcgcc gcgccgatcc gctgcaactg ccccgccatc ggcaggcgct
     7981 caacaatctg ccgcagatgc agggccgcgc gtatccgccg gctgtaaatc acgacggacg
     8041 aaacggccag tccggccaca atgagcccca gcggcagcgt caagtgcgaa aaatgcgcgg
     8101 aatcgcgcgt caccagtatg acggccccgg acatcaacac cagcccgatc aaaccgatca
     8161 cgcggtccag aaaaatggtc gtcaccgcct cggtcttgcg cttggtaaag cgcgtgacgt
     8221 aataggcttt aatgacatcg ccgcccgtca tgccgcccgg cattgcgaag ttgaaaaaat
     8281 ttcccgcgaa tgtcagcttg gtcgaatccc acagcgacaa gcgcaccttc tgcacccgca
     8341 tcatccagac caggcgcacg gccgacatta gcggcaccgg cataaatagc attatggccc
     8401 agaatgcctg ccagcggtcc atttgccgca tcacggtgcg gatgccgtat tcaatgtccg
     8461 gaacgcgctg gcctttaacc tcttcgtgaa agatgtcggc cgccggaacg tcgatcactt
     8521 gtccgtcgcg cagcacgctg aaccgcccgt cggtctggcc cagcaggcgc accaacggcc
     8581 cgtccttgct tcccagccgg acgtagtcgt tccatttgac gttgaaggcc aagtacgcca
     8641 ccgcggccag gcatagggca attcgcagtg cagtcagggc ccagcggcgg cgcggcgaag
     8701 cgctcaattg aaactcccaa actctctcat cacggcatta cttcgtacac ctcaaacgcg
     8761 ccggcgccgc cctgctcccg atcgctgctc accagtttca ggccgccgcg agccagggca
     8821 tccgcccagg ccggcgtcac gtgcggcgga aagccgtagg accgccgcaa acgcgcgctt
     8881 tcagaccagg agaataccac gtgcgtgacg ccgtgggtac ggagccaggc gaccgcggcc
     8941 gaggggtccg ccgtggccga gaattccaac cacggatcct tactgaaagg cacggtgtag
     9001 tggcagcggc gcgtcacgta gaaggcccgc gcgtcgccga ctatccatac gtaagcgccg
     9061 gtcggcacgg cggtgttgac cgggtttacg ttgcgcaaaa tgtccgcgcg atcgatcatc
     9121 tcgttcagcc gcacgccggt cagactttgc caggacgccg cctcgtcgcg cagcatgttc
     9181 acaagcttga ggtcgccaag cagggcgccg gccaggctga agccgataaa cacggccaac
     9241 gccggtcgtg gtctggggaa actgccggcg ctgagttcta cgcctcggcc gacgagcagc
     9301 gccagcggga tgatgaccgg cacggcgaag cggcccggca tgtgcgtaaa tgccgcccag
     9361 gcgatcacga tcaatgccgc ccaaatcgcc agcacagacc agaaccgggg cgtgtcgccc
     9421 cggagcgggc cggtcagagc cacgtttgcc ctcgctggcg cttcgggctc ggatccctgt
     9481 gcagcgcgct tggtcgccag gagcgctagg ccgcccaggc ccaggagcca gagcgcctgg
     9541 ttgtacatac ccgagttgag tagctcatca aacgcgattc gcgcccgggc ccccagcgag
     9601 gccgactcca ccggcaggct gtggccgcgc tgccattgct cggcctgtgc cgcgctccag
     9661 tcccggccgc cgaaccagcc gtacgcgaag gggtatacgg ggttgccggt gaaagctgca
     9721 ttgcgaatca gccacgggct gaatgccagt attgcgccga aaacgtacaa ccccagacgt
     9781 accgcgcgag tttttggacc gtcgcgcatc ccgatcagcc acgccgatcc cagcgcgccg
     9841 gccaccagca ccaaggccgt gtatttgcag ccgccagcca gcccagcgca tatccccgcc
     9901 gcaaaggccg ttcgcagtgt gggcttttcg gaaattagca gcccagccgc cacggccgcg
     9961 aagaacagca cccccaattc gacatatgcc aaagacccga ggtacgccag ccacacggtc
    10021 gtaccggcaa ccagtgccac aaggacgcgc ggccagccgc gcggcgtcca aagcagcagg
    10081 gccgcgaccg tcagcccgcc taaagccgcg tgcaacaatt gggccggaat cgccgcggcc
    10141 agcggcccgc ccgccaggta catcaacagc aggtagagcg tttccacccc ctgtggaaat
    10201 gacgcgtaaa cgttatgcgg cagaaacaga atccgtcccg cgtcgaagta ctcgcgcggc
    10261 acttgcaggt gatattcgag cacgtcataa ccgcgcgcct cgcccgccca aagcagcccc
    10321 ggcggcaagc acgcggcgta tagcacgacg acgatgggaa cgcacagcgg cagaagcacg
    10381 agcgttcttg ccagcagccc cgggcctgca acgctgtcgc tatcgacgtt gccgagctga
    10441 ccgcgcgcgg cgcgaagcag ccgaaccgcg cccagcccac cccctgccgc cagtagcgcc
    10501 catgccacgg gcccgctcaa caggcctgcc accccgagcg caagcatcgc ggtgctgagc
    10561 acccccagcc ccagcgccat agccaggcaa acctgccgtc cagccgattg cccggctgcc
    10621 agccacgccg caggccaagc gccccagcca gccgcgccca gcagcagcgc cgcaacgtac
    10681 gcgccgtcga ccggcagcac cagccatacc gcgggccagc aaacagccaa tactgcgcct
    10741 ggcacgacaa gaagaaccag gctcgcaagc ctgtcgcccc tggagagacg gggtatttgt
    10801 gccacggcgg attccgaacg gagcctcatt cgacgctcaa gttcggcgga ttggctcgag
    10861 ccgtcaagcg gtaaactggc gtaaaccgta gcagagccgg ggcgtgtcgc cccggaatgc
    10921 cgccagcagt ctcacacccg tgcgccgtgc cgccccgaaa tgcctccaag gatcctcttc
    10981 agcgtcgctg cgacccagtc ggggcgctcg cgtacagccc gtgccgcgcg atgtaggacg
    11041 ccacgggttc cggcgtgaga tagcgaacgc tctgcccggc tcggacgcgg ctgcggatat
    11101 ctgtcgcgct gatctcgatg cgcggactgg cgatgacgtg ctgccgtagg cgctctatct
    11161 gggaatgtgt cagcaagcgc gccagatcag gcaggtcacc ggcctcaaac cccggacggg
    11221 ccgccgtcac gatcgtgcac gcatcgacca gttcgctg
//
LOCUS       DAOSYS010000111        11190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_4967_length_11190_cov_4.942075, whole genome shotgun sequence.
ACCESSION   DAOSYS010000111 DAOSYS010000000
VERSION     DAOSYS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4967_length_11190_cov_4.942075"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..2303)
                     /locus_tag="PLU99_08360"
     CDS             complement(54..2303)
                     /locus_tag="PLU99_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17108.1"
                     /translation="MLANHVLRVSAVALAMLLVPAGPTTWAACVGEWVYAPGQTLPGV
                     HGSVFSTAIYDDGNGPALYVGGEFDFAGDGPAANIARWDGTRWSALGKGIRGTVSALC
                     VYRGDLIVGGQFATAGGVSANCIARWNGTTWAALDAGLEGSSAAVTALAVYDDALIAG
                     GLFELAGGTSAANIARWDGESWQPLGSGLDAGVGALTVWNGELVAGGGFLTAGGVPVE
                     HIARWNGATWSAMGSGMPASVNTLAVWGADLVAGGRSGGFPQPFILRWDGAAWAPIGA
                     GLSGFNGYVKSVVDYNGLLIVGGKFDRSGTTTLNGIAAWDGQAWQSIAEPWAVSAVTT
                     LGVWGDELIVGATSQIGAGLVRWNGSQWDGVGGTGQIYGGPVQAFCEYQGELIAGGKL
                     NLGGSLSTSIARFDGERWHAMGTGLMGELEHYVYALAAYDGSLIAAGSFLWAGEIEVN
                     YVARWDGADWHPMGDGFDSYVNALAVYKGELIAGGWFENSGLTPTPYLARWTGVDWQP
                     LGGGTSGEVHSLYVWNDDLIIGGRFNMAGDVAANCIARWDGETFHPFGLGMNFRVLAL
                     TEFQHQLIAGGSFSRADGRPVHGIARWDGDTWHAMGIGPVMDLAAYHGELWGTRAVST
                     LDSTGYQLIRWDGTDWRLQPGVFDGNVSALDVWENTLLVGGSFMTVASRAAPVWVRMA
                     CPFRHGDLNCDAAIDVFDIDPFILALTDPAAYEWQYPQCDQTLADINNDGLVNTFDID
                     PFVLLLTAR"
     gene            complement(2593..>4035)
                     /locus_tag="PLU99_08365"
     CDS             complement(2593..>4035)
                     /locus_tag="PLU99_08365"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOO17109.1"
                     /translation="MNLQEDLHWALGRDRKLASIGVYDLDTLEGDVFHYDAVDPDSLR
                     FVPLGFPPDEPDSLLTPREILERHKTGQAYAHLLKPLRKYPLLRDGAGTVLSMPPIIN
                     SEGTRVTMNTRQFFIDVTGLSQRSVDRALNIVVTSLAESMPEVVIESVVIEEMDQGTE
                     GAKTRENGQPKGKRNADPHSAESGAARPRTRITPDLTPTEMMLDVKTAAETIGVELNA
                     GRLAELLESMGYGVREAGRADRLSVLVPAYRNDVMHAVDLIEDAAIAYGYEHITPELV
                     PTFTVGSPRAIEEQAATARRVLTGLGFHQVMTLVLTNEEAAFRKWRLEPATNTVKIEN
                     PISVEQTICRVSLLPGLLETLAINRQYDLPQHLFETGDCSFVDAAAETGAREERFVAA
                     AMIGTHVGYADIRAVADAFVHEMGRTYVVKPIEHASLIPGRAAGLFDASGRQIGLMGE
                     LHPEVLENYGLRHPVAVLELSLEKLLAQAS"
     assembly_gap    4036..4135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4136..4700)
                     /locus_tag="PLU99_08370"
     CDS             complement(<4136..4700)
                     /locus_tag="PLU99_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17110.1"
                     /translation="MPIIDLPVDLLLARVRAHGGAGDVGLDDLVEILPKLGCEVEEVA
                     ETQQYSCGVCAKIYDRTPAQGAPLVCSHCGADFRADPDQLDDLGPNTVLRLNMLAVRP
                     DIFDPGGMARYIRGYLGVDSGLAEYAVTAPRLRVQVDPQLSNEDSFRPHIACAVVRNV
                     RLNHGTIKLLMNLQEDLHWALGRDRKLA"
     gene            complement(4703..5779)
                     /locus_tag="PLU99_08375"
     CDS             complement(4703..5779)
                     /locus_tag="PLU99_08375"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOO17111.1"
                     /translation="MPFAEIGLRRADGGHQPLAIQIDSGAVISLLRRSAAELLGIDLQ
                     TGEKIDFGSVGGNRVCAFRHDLQARFSDGFEMRVPFAIAEREDVPNLLGRLGIFDKLQ
                     VDFDATLQETRISASWLCQNKRRIWDSLVEVGNHVLARWKTNPLPGRADEASSRLVRR
                     SAQLLAAIAGLIKLGRGYEAPVMLRAMIEVSVQLEYLLLNPQERAELYLEYEHVSNYR
                     YMSALLTDCRPGTISGGLANSPQRAGGEATARANYERVRARFTKEGKSAPAGKWYMKS
                     FRELCEETGHLAEYKLWYSRFSAWLHADPSAIRQQVPLPGNHALMLAMCYHGGVLRQI
                     ADRKKMVLTNEQYEFLNALAKNIY"
     gene            complement(5820..6032)
                     /locus_tag="PLU99_08380"
     CDS             complement(5820..6032)
                     /locus_tag="PLU99_08380"
                     /inference="COORDINATES: protein motif:HMM:NF039192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5678 domain-containing protein"
                     /protein_id="HOO17112.1"
                     /translation="MSSDFAWLVDHSDEIAQLYAGKWIAVRDGQVVGVGDTAVEAAEQ
                     ARAQDPEAEFILEAVDRDADVVYADV"
     gene            complement(6103..7641)
                     /locus_tag="PLU99_08385"
     CDS             complement(6103..7641)
                     /locus_tag="PLU99_08385"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOO17113.1"
                     /translation="MELPQVQFDVLRFLSEAGRPVAIGDIAAALQLDQSPVMAAAIVL
                     QEAGLVRVDESAYEEFRLGPEGEQFVKQPLPERVIMGVLAQLGGRAAITDIPLHSNLN
                     AGQVGQTLRWLAQRGWARKEGGELVLTATAEAAAAGPPQQPDEKVIQALAGGRVATRA
                     ELEGEGLPLAEALELLSKRKGFLAIKERTARTAAATEKGRDLIAQGVESRRRVTQLTP
                     ELLTDGAWRGVELQPYDVTLAAKKLYPGKEHAFQRTLDRVRRVFLEMGFEEIVSPWVE
                     SSFWDFDALFQPQDHPARDMQDTFYVARPDRCRLPDDELVERIRRTHEDGGDTGSTGW
                     QYRWSRDLATRPVLRTHTTAATMRALARHTAGRPGKYFIIGPVFRRETADFKHLPVFH
                     QVDGIIVDKHASLATLIGTLSAFYAKMGFPKVKVTPSFFPYTEPSAEVHLYMEARGEW
                     VEMGGSGLFRPEVTLPVGITDRVLAWGLGLERLAMMIHNLGSIGELYFATMPWLREEA
                     LSRA"
     gene            7946..10129
                     /locus_tag="PLU99_08390"
     CDS             7946..10129
                     /locus_tag="PLU99_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOO17114.1"
                     /translation="MFASLGRAIGRHPGPIVAGWVAVLVAAAVGQRVLPAPQSPNAGA
                     VLPPGSAYIRAKELYQQAFPQLAAESTLAVIAQRTGGITAADLQRLAQLADDVRQATG
                     APVLSPASPLLRRRLLSPDGQAAMLVVNLPTSFVSPHTLAATERVLDLAHADAPPGLR
                     IEVTGTAAMGRDYVRASQTGAHRTTWVTISAVLIILIAVYRSPVGALVPLISIGASAY
                     LAYVALEISRLLGWQFGATEWVFCVVLIFGAGTDYALFWIARYRETLAARTDFSFAAQ
                     TAMQTTGPAILTGAAVTICGLSTLMAAELVPTHNAGRVLAAALTINLAACLTLTPALA
                     RLLERWLLWPADAAAQPGLGARHWWPWLARNVTTHPWPVLLAGTATLAIAAALSLRLK
                     PIFKGVFDLPSDAPSKRGLRLAEEHFSAGQLYATTLLIELGPGLEAEWESLSARVTAA
                     VQTIPGVAEVYSLTSPAAPGGQLPSAVQSLARPYYVSQAADGSTVMRIEMLPNDAPFA
                     PQTLQAVERAAAVARKVSMEALPPQAGRPRVMLAGPSAYILNERTIADADQRRVVVLA
                     AAVIALIVLMLVRDVALTAFMVLTTLLTYGATLTLSSLFFVHAMGLPGLDWKVRLILF
                     VIVMAVGQDYNIFLVARLREQIHRQDRAQAVRAAVVRTGPVISSCGIIMAATLGSLWA
                     GRLSLLQQLGFALALGVLIDTFFVRPLLIPAFFMVIGNVRSARQN"
     gene            complement(10821..>11190)
                     /locus_tag="PLU99_08395"
     CDS             complement(10821..>11190)
                     /locus_tag="PLU99_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17115.1"
                     /translation="NAEKEPALSPDNPERRGGDGTRAGEATGQTMSAGNPATGAPAGS
                     NELDDLPAPIVDKPLFSADAVPADWPPKLRERFRVLRRVSRKSDAELAEQVWAERERV
                     LAAPPQPSGQGKRKRRFWGN"
BASE COUNT         1936 a         3706 c         3415 g         2033 t
ORIGIN      
        1 cgccccgaaa cgccgccagg aatctcccgc cgccgcagta cggattatgc ccgctaccgg
       61 gcggtaagca gcagcacgaa cgggtcgata tcgaacgtgt ttaccaggcc gtcgttgttg
      121 atgtctgcca gcgtctgatc gcattgcggg tactgccact cgtaggccgc cggatcggtc
      181 agcgccagaa tgaacgggtc gatgtcgaac acgtcgatcg ccgcgtcgca attcaggtcg
      241 ccgtgtctga acgggcaggc catgcggacc cacacgggcg cggcgcggct ggcgaccgtc
      301 atgaacgaac cgcccaccag caacgtattc tcccagacgt ccaaggctga aacgttccca
      361 tcgaaaacgc cgggctgcag ccgccaatcc gtgccgtccc agcgaatgag ctgatatccg
      421 gttgaatcca gcgttgaaac ggcccgggtg ccccatagct cgccatggta ggcggccaag
      481 tccatcaccg ggccgatgcc catcgcgtgc caggtgtcgc catcccagcg ggcgatgccg
      541 tggacgggac ggccgtcggc gcggctgaag gaaccgcccg cgataagctg gtgctggaac
      601 tccgtcagcg ccaggacgcg gaagttcatg cccaggccga atgggtgaaa agtttcgccg
      661 tcccagcggg cgatgcagtt ggccgccacg tcgccggcca tgttgaaccg gccgccgata
      721 atgagatcgt cgttccagac gtacagcgaa tgcacctcgc cgctggtgcc gccgccgagc
      781 ggttgccaat cgacgccggt ccagcgcgct aggtaaggcg ttggagtcag gcccgagttt
      841 tcgaaccagc cgccggcgat cagctcgccc ttgtacaccg cgagcgcgtt gacgtatgaa
      901 tcgaatccat cgcccatcgg atgccagtcg gcgccgtccc agcgcgccac gtagttgacc
      961 tcaatctccc ccgcccagag aaagctgccg gcggcgatca gactgccgtc gtaggccgcg
     1021 agcgcgtata catagtgttc caactcgccc atcagtccgg tgcccatggc gtgccagcgc
     1081 tctccgtcaa agcgggcgat ggacgtggac agactgccgc cgaggttcag ttttccgccg
     1141 gcgatcagct cgccctggta ttcgcagaat gcctgaaccg gaccgccgta tatctggccg
     1201 gtgccgccta caccgtccca ttgcgagccg ttccagcgca ccaggcccgc tccaatctgt
     1261 gaggtcgcgc cgacgatcag ctcgtcgccc cagaccccga gcgtggtaac ggctgacacc
     1321 gcccacggct ccgcgatcga ttgccaggcc tggccgtccc acgcggcgat gccgttcagc
     1381 gtggtcgtgc cgctgcgatc gaattttccg ccgacgatca gtaagccgtt gtaatcgact
     1441 acggatttca catagccgtt gaagccggac agacccgcgc caatgggcgc ccacgcggcg
     1501 ccatcccagc gcagtatgaa cggctgcggg aagccgccgg atcgcccccc ggccaccagg
     1561 tctgcacccc aaaccgccaa agtgttaaca ctagccggca ttccgctgcc cattgccgac
     1621 caagtcgcgc cgttccagcg cgcgatgtgc tcgaccggca cacctccggc cgtgagaaag
     1681 ccgccgcccg cgactagctc gccgttccag acggtcaggg ccccgacgcc ggcatccagg
     1741 ccgctgccga gcggctgcca actttcgcca tcccagcgcg cgatgttggc ggcgcttgtg
     1801 cccccggcca actcgaaaag gccgccggcg atcagcgcgt catcgtagac ggccagcgct
     1861 gtgacggcgg cggacgagcc ctccagaccg gcatccagcg cggcccaagt tgtcccgttc
     1921 cagcgtgcga tgcagttggc gctaacgccg ccggcggtcg cgaattggcc gccgacgatc
     1981 aaatcgccgc ggtaaacgca aagcgcggag acagtgcccc ggatgccctt accgagggcc
     2041 gaccagcgtg tgccgtccca gcgggcgatg ttggcggctg ggccatcgcc ggcaaagtcg
     2101 aactcgccac ccacgtacaa agccggtccg ttgccgtcgt cataaatcgc agtcgaaaaa
     2161 acggagccgt gcacgcccgg cagtgtttgg ccgggtgcgt agacccactc ccccacgcac
     2221 gcggcccacg tcgtagggcc ggcaggcacc agcaacatgg ccagcgcaac cgcggaaacc
     2281 ctgaggacgt gattggcaag catctatcac ctccaaccgc cggataaggc tagccgaacc
     2341 ccgattgtaa caatttcggg tgagcattag gacaggtgaa tgcgtggccc agctcatgac
     2401 agttgagcgg gcctcgcagc gattatcgga aggatgccct ccgcgcgcat cctgcagcaa
     2461 caggcgttaa gcccgacgtg ctggaggcgg caccactttc ccactttccc acgttcccac
     2521 tttcccacgc tgagtagcgt cggagcagct cgcgacgtgg gagacgtggc tgcgcggcgt
     2581 cgacagcctc gcttagcttg cctgcgcgag cagcttctcc aggctcagtt ccagcacggc
     2641 caccggatgc cgcaatccgt agttttccag cacctctggg tgcagctcgc ccatcagccc
     2701 gatttgccgc ccgctcgcgt caaacaggcc ggccgctcga cccgggatca ggctcgcgtg
     2761 ttcgatcggt tttacgacgt aagtgcggcc catctcgtgt acgaaggcgt cggctacggc
     2821 gcggatatcg gcgtagccca catgcgtgcc gatcattgcc gccgcaacga aacgctcctc
     2881 gcgcgccccg gtttcagcgg cggcatcgac gaaggagcag tcgccggtct caaacaggtg
     2941 ctgcggcagg tcgtattgtc tgttaattgc caacgtctcg agcaggcccg gcagcagcga
     3001 aacgcggcag atggtctgct ccacgctgat ggggttctcg atcttcaccg tgtttgtggc
     3061 cggctccaga cgccatttgc ggaaggcggc ttcctcgttg gtgagcacga gcgtcatgac
     3121 ctggtggaac cccaggccgg tcagcacgcg tcgcgcggtg gccgcctgct cttcgatcgc
     3181 gcgcggcgaa ccgacggtga aggtggggac gagttcgggc gtgatgtgct cgtagccgta
     3241 ggcgattgca gcgtcttcga tcaggtcgac cgcgtgcatc acgtcgttac ggtaggcggg
     3301 cacgagcacg ctgagcctgt cggcacgccc cgcctcgcgc acgccgtagc ccatggattc
     3361 gagcaactcg gccaggcggc cggcgttgag ttcgacgccg atcgtttcgg ccgccgtttt
     3421 gacgtcgagc atcatctcgg tgggcgtcag gtccggggtg atgcgggtgc gggggcgcgc
     3481 agcgccggac tcggccgagt gcgggtctgc gttcctcttg cccttgggct ggccgttttc
     3541 gcgggtcttc gccccctccg tcccttgatc catctcttcg atgacgacgc tctctatgac
     3601 tacctccggc atactctcag ccaggctggt cacgacgatg ttgagggcgc gatcgaccga
     3661 ccgttgcgat agcccggtca cgtcgatgaa gaactggcgc gtgttcatgg tcacgcgcgt
     3721 tccctcgcta ttgataatcg gcggcatgga gagcaccgtg cccgccccat cccgcagcaa
     3781 cgggtacttg cgcagcggct tgagcaggtg cgcgtacgcc tgcccggttt tatgccgctc
     3841 gagaatttcg cgcggcgtga gcaggctgtc cggctcgtcg ggcggaaagc cgagcggcac
     3901 aaagcgcagg ctgtcgggat cgaccgcgtc gtagtggaac acgtcgccct cgagcgtgtc
     3961 caggtcgtaa acgccgatcg aggccagctt gcggtcgcgc ccgagcgccc agtgcaggtc
     4021 ttcctgcagg ttcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaggcc
     4141 agcttgcggt cgcgcccgag cgcccagtgc aggtcttcct gcaggttcat gagcagcttg
     4201 attgtgccgt gattcaaccg cacgttgcgc acgacggcgc aggcgatgtg tggccgaaag
     4261 ctgtcttcgt tcgaaagttg cgggtccacc tgcacgcgca gccgcggcgc ggtgacggca
     4321 tattccgcca gcccactgtc gacgcccagg tagccgcgga tatagcgggc cattccgccg
     4381 ggatcgaaga tatcggggcg taccgccagc atgttcaggc gcaggacggt attgggcccg
     4441 aggtcgtcga gctgatccgg gtcggcgcga aagtcggcgc cgcaatgcga gcagaccagc
     4501 ggcgccccct gggccggcgt gcggtcgtag atcttggcgc agacgccgca ggagtactgc
     4561 tgcgtctcgg ccacctcctc gacctcgcag ccgagcttgg gaagaatttc gacgagatca
     4621 tccagcccca catcacccgc gccgccatga gcacgaacgc gggccaggag caggtcaacg
     4681 ggtagatcga ttatgggcat agtcagtata tgttcttagc taatgcattc aaaaattcgt
     4741 attgctcgtt cgtgagcacc atcttcttcc gatccgctat ttgccggaga actcctccat
     4801 gatagcacat ggcgagcatt aaagcatgat tgccaggtaa cggtacctgc tgcctgattg
     4861 ctgaggggtc ggcgtgcagc caagcactga acctgctgta ccatagtttg tactctgcaa
     4921 ggtgacccgt ctcctcgcat agctcgcgga acgacttcat gtaccatttg ccggccggtg
     4981 cactcttccc ctctttcgta aagcgtgcgc ggacgcgctc ataattggct cgcgcagtgg
     5041 cctctccacc ggcgcgctgc ggcgagttgg ccaacccccc agagattgtg cctggacggc
     5101 aatctgtcag caatgctgac atgtatcggt aattagaaac gtgctcgtat tccagataca
     5161 gctccgcgcg ctcctgtgga ttcaggagca aatactccag ttgaacgctg acctcgatca
     5221 tcgctctcaa catcacgggg gcttcgtaac ctcggcccaa ctttatgagt cccgcgatgg
     5281 ccgccaggag ttgcgcgctt cgccggacga gccgggagct cgcctcatcg gctcgcccgg
     5341 gtagtggatt cgttttccag cgtgccaaaa cgtggttgcc aacttctaca agcgaatccc
     5401 agattcttct cttattctga cacaaccaag aggcagatat tcgggtttcc tgcagggtgg
     5461 cgtcaaaatc gacctgcagc ttgtcgaaga tgcccagtcg cccgagcagg ttaggcacgt
     5521 cctcccgctc tgcgatagcg aacggaacgc gcatctcgaa gccgtcgctg aagcgcgctt
     5581 gtaagtcgtg ccgaaacgcg cagacacggt ttccgcccac tgagccgaag tcgatctttt
     5641 ccccagtttg gaggtctatg cccagcaatt ccgccgccga gcgacgcagc agcgaaatga
     5701 cggctccgga gtcgatttga attgccagcg gctgatgtcc cccatcggcg cggcgaaggc
     5761 caatttccgc gaacggcacc cacacttcgc cgaagtggcg agttcgcctc cggcgccact
     5821 taaacgtcgg catagacgac gtccgcgtcc cggtctaccg cctcaagaat gaactcggcc
     5881 tcggggtcct gcgcgcgcgc ctgctctgcc gcctcgaccg cggtgtcgcc gaccccgacc
     5941 acctgcccat cacgcacggc gatccactta cccgcgtata gctgggcaat ttcgtcgctg
     6001 tggtccacga gccaagcaaa gtcggacgac attttcgcac ctctaatggt agtataccga
     6061 attttgggcg gattgcacgt attcccgtcg cgcctcgcgc ccctacgccc ggctcagcgc
     6121 ctcctcgcgc agccacggca tggtcgcgaa atacagctcc ccgatgctgc ccaggttgtg
     6181 gatcatcatc gccagccgct ccaggcccag cccccaggcc agcacgcggt cggtgatgcc
     6241 gaccggcagt gttacttccg ggcggaacag gcccgagccg cccatttcga cccattcgcc
     6301 gcgggcctcc atgtacaggt gtacctcggc cgacggctcg gtgtacggga agaagctggg
     6361 cgtgaccttc accttcggaa agcccatctt ggcgtaaaac gcgctgaggg tgccgatcag
     6421 cgtcgccagg ctggcgtgct tgtcgacgat gatgccgtcg acctgatgga acaccggcag
     6481 gtgcttgaag tccgccgtct cgcgccggaa taccggcccg atgatgaagt acttgcccgg
     6541 acgccccgcg gtatgcctcg ccagcgcgcg catcgtggcg gccgtcgtgt gcgtgcgcag
     6601 tacggggcgc gttgccaggt cccggctcca gcggtattgc cagccggtcg agccggtgtc
     6661 gccgccgtcc tcgtgcgtgc ggcggatgcg ctcaaccagt tcatcgtccg gcaggcggca
     6721 gcggtccggc cgggccacat aaaacgtgtc ctgcatatcg cgggccgggt gatcctgcgg
     6781 ctggaacagc gcatcgaagt cccagaagct cgactcgacc cacgggctga cgatttcctc
     6841 gaaacccatc tccaggaaca cgcgccgcac gcggtccagc gtccgctgaa atgcgtgctc
     6901 cttgcccgga taaagcttct tcgccgccag tgtgacgtcg tacggctgca actcaacccc
     6961 gcgccaggcg ccatccgtca gcagttccgg cgtgagctgc gtgacacgtc gccgagactc
     7021 tacgccttga gcgatcaggt cgcgcccttt ttcagttgcg gcggccgtcc gcgccgtgcg
     7081 ttccttaatg gccaaaaaac ccttgcgctt ggagagcagc tcgagcgcct ccgccagcgg
     7141 cagaccctcg ccctcaagct cggcacgcgt cgccacccgc ccgccggcga gcgcctggat
     7201 caccttctcg tcgggctgtt gcggcggacc ggcggcagcc gcttcggcag tcgcagtcag
     7261 aacaagttct ccaccctcct tgcgcgccca gccgcgctgc gccagccagc gcagcgtctg
     7321 tcccacctgc ccggcgttca agttgctgtg cagcggtatg tccgtgattg ccgcgcgccc
     7381 gcccagttgc gccagaacgc ccattatcac gcgctcgggc aaaggctgct tcacaaactg
     7441 ctcgccctcg ggcccgagcc ggaattcctc gtaagcggat tcatccaccc gcaccagccc
     7501 ggcctcttgc agcacgatgg ccgccgccat aacgggcgat tgatccagtt gcagcgccgc
     7561 ggcaatgtct ccaatcgcca cgggccgccc cgcttcggac agaaatctga gcacgtcaaa
     7621 ttgcacctgc ggcaattcca tcttgcacct cgaaagaaag tgcttggtgt accccggcgc
     7681 gattcggcgg acaagtcttt cggcgccaac agaacagatt aacagcggga cagccatgcc
     7741 caaggtgctt ttgcccaagg tggtaccgcg cccaaggtag gcgccatcct taaggtaggc
     7801 gccatgctta gggtgggtgc catgcccaag ccgcgtagcg gcgcgggcat gcctccgcag
     7861 ggcacccccc ggcgcgccct atactcctta gcgttcgcga ttctgatgaa cgctatctca
     7921 gggcagcgcg tagagtgaag ctgtagtgtt cgcatctctg gggcgagcaa tcggaagaca
     7981 ccccgggccg atcgtcgccg gctgggtggc agtgctggtc gccgcggccg tcggacagcg
     8041 agtcttgccc gcaccgcaat cgcccaatgc cggagccgtg cttccacccg gctctgctta
     8101 cattcgggca aaggaacttt accagcaagc cttcccacaa ctcgccgcgg aaagcacgct
     8161 cgcggtaatc gcacaacgaa ccggcggcat caccgcagcc gacctgcagc ggctcgcgca
     8221 gcttgcggac gacgtccgcc aggcgaccgg cgcgccggtc ctctccccag cctcgccgtt
     8281 gctgcgccgg cgtttgctaa gccctgacgg ccaggctgcg atgctggtcg taaatctccc
     8341 gacgagcttc gtaagtccac acaccctggc ggcgaccgag cgcgttttgg acctggcgca
     8401 tgccgacgca ccaccgggtc tgcgaatcga ggtgaccggc accgctgcaa tgggccgcga
     8461 ctacgtgcgg gccagccaga cgggcgcgca ccgcacgacg tgggtaacaa tctcggccgt
     8521 gttgatcatt ctgatagccg tgtatcgatc accggtggga gcgctcgtgc cgctgatctc
     8581 gatcggggcc agcgcgtacc tcgcctatgt cgctctggaa atatcgagac tgctcggctg
     8641 gcaatttggt gccaccgagt gggtgttctg cgtcgtgctg attttcggag cgggcactga
     8701 ttacgccctg ttttggatcg cgcgctatcg cgaaacgctc gctgccagga ctgacttcag
     8761 cttcgcggcg caaaccgcca tgcagactac gggaccggcc atcttgactg gagcagccgt
     8821 caccatctgc gggctatcga ccttgatggc ggccgaactc gtccccacgc acaacgccgg
     8881 ccgcgtcctg gcagccgcgc tcacgatcaa cctggcggcc tgtctcacgc tcacgccggc
     8941 tctggcccga ctgctggaac gctggctcct gtggccggcg gatgcggccg cacaaccggg
     9001 actgggcgcc cgccactggt ggccgtggct ggcgcggaac gtcaccacgc acccctggcc
     9061 ggttcttctg gcaggcacgg ctaccctggc aatcgcggcc gcgctttcat tgcggctgaa
     9121 gccgatcttc aaaggcgtct tcgatttgcc gtccgacgcg ccctccaagc gaggcttgcg
     9181 gctggcggag gagcatttct ccgcgggcca gctctatgcc accaccctgt tgatcgagct
     9241 tggcccgggt cttgaggctg aatgggaaag cctgagtgcg cgcgttacgg ccgcagtcca
     9301 gactataccc ggagtggccg aagtgtactc gctgacttcg ccggccgccc ccggcgggca
     9361 gttaccatct gcggtgcaat cgctcgcacg cccctattac gtgtcacaag ccgcggacgg
     9421 gtcaacggtc atgcgaattg aaatgctgcc gaatgacgcg ccgtttgcgc cgcagacgct
     9481 tcaagcggtc gagcgggccg cggcggtggc gcgaaaagta agcatggaag cgctgccgcc
     9541 gcaggcaggc cggccgcgcg tcatgttggc cggcccgagc gcgtacattc tcaacgagcg
     9601 caccattgcc gatgcggatc agcgccgcgt cgtggttttg gcggcggcgg tgattgcgtt
     9661 aatcgtgctg atgctagtgc gtgacgtcgc gctgactgcg ttcatggtac tgacgacttt
     9721 gttgacgtat ggtgcgacgc tgacgttgag cagcttgttc ttcgtgcacg ccatgggttt
     9781 gccgggtctg gattggaaag tccgcttgat tctgttcgtg atcgtcatgg cagtggggca
     9841 ggactacaac attttcctgg tggcgcgctt acgcgagcaa atccaccggc aggatcgggc
     9901 gcaggcggtg cgcgcggcgg tagtgcgcac cggccccgtc atcagcagtt gtggaatcat
     9961 catggcggcc acacttggtt cgctttgggc gggccggctc tcactgcttc agcaactggg
    10021 ctttgccctg gcattgggcg tgctgatcga taccttcttt gtacgcccac tgctcattcc
    10081 ggccttcttc atggtgatcg gcaacgtgcg cagcgcgcgg caaaactgag ggcccgcaaa
    10141 ccgccgcggg cgccgcgcaa ggctgactta gacgctggcc ggctgttagc cgggcgctgc
    10201 gccgacgaga ttagcggggc cgttgcgcct ttgcgcgcct ggcgcagggt tagcttcaag
    10261 gggggcgggc atcaggacag ccgatgccca accccgcctt gaaacttgcc ggactggaac
    10321 gcactgagcc cgctggctga actaccgggt gggcagtcgc cgccgctcag aaggttggct
    10381 aaagtgtcaa aaccaaacgt cctgctcatt ggcggacacc cagctcctcc aaagtgcctt
    10441 cgagttgaaa cctaatgatc ccgcggaatg ccggcctaac gaacgcggcc gacctcgatg
    10501 acctctgctg atcggcactg ccggtccgac cgcgggcaat acgctaaagg ctatctaaat
    10561 tctacagtat tagcggagaa aagcaaaaaa atccgataca aacgggcgca aaacgccaaa
    10621 tttccgctcg tttctggcat agtgttttcc gacactgtca gtcggcttta gtcactcgcc
    10681 cactgcggca atggcagcag gccaatcgca gagccggcgg acacgtagct caaaagtgtg
    10741 aatgcaaatg ggagcggttt cgtgaggttc gcttgcaacg ttttgcgtga gcaatcacca
    10801 ggaggctcac tgcaccggcc ttaattcccc caaaagcgcc gcttgcgctt cccctgcccg
    10861 ctcggctgcg gaggcgcagc tagcacgcgc tcgcgctctg cccacacctg ctcggccagc
    10921 tctgcgtcac tcttgcggct cacgcgccga agcacccgaa accgctcacg aagcttcggt
    10981 ggccagtcag cgggcactgc gtccgcggag aataggggct tatcgacgat cggcgccggc
    11041 agatcatcca attcgtttga acccgctggg gcgccggtcg cggggttccc cgcgctcatc
    11101 gtctgtccgg tggcttctcc agcacgcgtt ccatccccgc cgcgacgttc cggattatcc
    11161 gggctcaatg cgggttcttt ttccgcgttg
//
LOCUS       DAOSYS010000112        11074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5031_length_11074_cov_4.831201, whole genome shotgun sequence.
ACCESSION   DAOSYS010000112 DAOSYS010000000
VERSION     DAOSYS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_11074_cov_4.831201"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="PLU99_08400"
     CDS             complement(<1..1819)
                     /locus_tag="PLU99_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOO17116.1"
                     /translation="MNSTKPEELEPAAAMGPLADSAIVSDISPEPGSGSQHGDRRRPW
                     VVAAALAGILLVGLAMRIWIVATPGLGLQDDLRIFSHWSRHLASDGLARFYATQQFCD
                     YPPLGVLVFYAVGKSTAAIGGGSASEELLHIILKIPACLGDLAIALLLFVEARRLLGS
                     RAGLGAAALYFLNPVALYNSAYWGQVDAIYTFFALLAVVLMARRHWYLAGAAVAAGLL
                     AKFQTIAFVPLLLFETYRLGGWRAIAGKLVAAVALAAVVTTPFTLTGTLGEVLQRGYV
                     NVIGQYKQLSKGAFNTWTLVAPADTTDAAAPVVLTRIAAAGQTEIPAGSSWLLALTWR
                     RISLLVYALSVAVVLAIYSLRPGPIARYAAAALLGLAFFLFPTEMHERYAFPALAFLA
                     LWAVSGQWRERAYFLLSALLLMNLTFVLPPGGLAPQIATASLLIFGVLLVALAWPQAV
                     REPSLPLRAPALPPKEIADEPSLSTSQPLIVWFRRATLGAVVIAVAGATSIAVLANRA
                     PEVAAATDAVYLSEMKPVRAQQAWRSLALDRAVAGGPLELGGTIYLRGLGTHAPSRIE
                     YAVPPNATRFRALAGIDHGTGGAGSATIHVEVDGKRVFTS"
     gene            complement(1819..4461)
                     /locus_tag="PLU99_08405"
     CDS             complement(1819..4461)
                     /locus_tag="PLU99_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOO17117.1"
                     /translation="MAGTVETGRDPSSRVLVIIESLALLVFGIALMTYFYHSSARGAG
                     DELGVPGHDSYYHIKMAALLPQVGIVHDLPWLQYAYFRQEGHEFVSHHFGFHVLLMPF
                     VYLSQWLTGDFLAGGRWAAATFFGLNLVLFNLLLRTGNVPYRWGWLVIFLLMPDQFFM
                     RHSLVRAIGASLLFMQLLLLLLFKGRYVWAALVAALYVQLYLGAVMFGPLLVGLYALA
                     QVLGPADDRRFPWRMVAWTAGGWALGVVSYPYFSGMLEFLILQVTKTGLAPDIEVGQE
                     WNPYVNTWWFLSMSAVPAVVWAGAVCLRMRRGPRLNAQELALLLINFAFLLLTFKARR
                     FIEYWPPFCVLSAAYLSAPVLAGLPALIPTLRPGETLHRNWTAWPILGLVAACIGYLL
                     WKGSERPDAPAVLADWRSWAAMLALLLLPALTQVWTAQRGERGQVPVLRLAALPATAA
                     VVFGCLALLVHFRFGVQNLPAARLDARPLWFVVAALYVIAPAAYHLTRRPMEASQPAP
                     VLVPAAAIVLVGLGLFGATLSVGSRQIRSVGEQMRTGFNLPAMRAALKFVEENSNPGD
                     IVFTDDWDIFPAYFYHNSHNYYVVGLDPKFTHARRPDLWERYMRITRGETPKQSVVKL
                     AGPDGQTRQETISIDLKDIRTEFKARFVICDDDHQSFASKLARDRNLAELVYPCKTYA
                     ACRNAPFLVFRIRDADEPPAAPATAPAPDLEGHVYLSALEPVSADQGWGELTSDASVE
                     NRRLRLRGTTYRRGIGTHAPSKLLYNVPAGYDWFEAVVGVDDETEGRGSILAAVYLDG
                     TRAFESPVLTGDSEPAVVRIPLAGARQILLVAETTADGQRFDHVDWADARFMHKPGTA
                     SQAAQPAANAGEEPGKEAITPGAP"
     gene            4627..6828
                     /locus_tag="PLU99_08410"
     CDS             4627..6828
                     /locus_tag="PLU99_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17118.1"
                     /translation="MPRKASQSATAEIPTCPTPGAEKPATAPTARFRHLLLLILAVGA
                     ACRAVILVDYLAHNPTAATPITDAAWYWNWAERIAAGELIGRMPFTSAPLYPYLLGLL
                     RALGGGLKLLYLAQTAVSLLTAFLLAEIGRRRFGEAVGLLAAALFLLLQEPASAALRV
                     LASTLELLTVVIVWGLLVRLQARPSSWAAHVAAGTGLGLLCLAFAPALLLLPAAVCWL
                     ALRTPRLPAIARAAAFAAAAAVVIAPATLHNWLGANEFCLIRTGAGLTLRQGNHPQSV
                     GAYTPIPGVSVQREKQYESAAEVYRQETGQQPTWAAVDGYFRQQAVSFWRANPGRALE
                     LAAAKLYATLTYQNYSDIYQTRAEIACGLTRALWLAPLPMPLLIAAALIGALYWLRRP
                     LFYAPEWMLLILPILVTVLFFYSPRYRLPAAPLLTVAAALALVRAWHWRRHRATGAAV
                     VGALAASLTLTAVNAACGRDALDYAGVYRALAEAYAQQDRMDDSTAMLRQAVQADPGD
                     VDSRICLADRLRSLDRVDEAASEYLQAFQLAPDDAALFSRTFSALARAQRLVEAEALA
                     ARVMQRHPADPVWLAALAEAKLGAGDLRGANECIAQALALAPENTRWRHLQVDVLIGL
                     QRFAEAERELERILKSAPDDCGALWRQGQIAGRTGRPAEARRLLEHALAVCPDNAPAW
                     HDLAVVNMQMNRPQAARDCLRKALQVNPRQELSRQLLAELDSLGTPPVTQP"
     gene            6964..7482
                     /locus_tag="PLU99_08415"
     CDS             6964..7482
                     /locus_tag="PLU99_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOO17119.1"
                     /translation="MIQHTHAQTAQTADVLHGVRQHVLWLLDGGNAHLDFDAAVAGLP
                     AKLRGAKPINVPYSPWRLIEHMRLSQWDILEFCRNPKHVSPEWPEGYWPQSDAPPDER
                     AWEASLREFHDDLAAMRALIKNPQANLLAPLPHGEGQTLLREALLLADHNAYHLGQLV
                     TVRRALGAWPEE"
     gene            7747..9063
                     /locus_tag="PLU99_08420"
     CDS             7747..9063
                     /locus_tag="PLU99_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOO17120.1"
                     /translation="MNESDARKEHKPKIFSADPIKQAGPAPWSARRRRPNLLVLGASG
                     HVAGAFLRRLGGRRDLFNEVVLLDRNEQVVENRFLEHSRLRYTFVHHDLRFRDHDTDY
                     VRLLQQRQIDIVLDLTDLDTMPVFQATDAAGVRYINTALNDANAGVHEMLARIHPVRQ
                     RPRRAPHIISSGMNPGAVNIWVMHGVRNYGVPREIIHFEYDSSMSFDRWRPLITWSRR
                     EFLTETVWEPTGQVDHGRVRVFDSNALANRVNLGPIMEPVHKLPSYPRGFLVLHEENI
                     KLAQKFGISSKYIYALHPKTMDFLVRRWREHGTVLISDLEVGDNTSIPLTGSDTIGVC
                     LQYPRKRVYYVHSLSNQSVVGTNATCAQVAIGVWAALTTMLTEPLQSRVYFPTDLYGT
                     IYPHVLFSNQRVEVFECGWYNKTWTVLKHIPELHPRLSRGKEQSII"
     gene            9135..>9431
                     /locus_tag="PLU99_08425"
     CDS             9135..>9431
                     /locus_tag="PLU99_08425"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO17121.1"
                     /translation="MKSFLAITRALSDETRLRALLALRQGELCLCQIISLLGMAPSTV
                     SKHMDLLYQAGLVERRKQGKWHYFRLAGREAPAAAQKAIRWALDALANEPLVKAD"
     assembly_gap    9432..9531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9532..10244
                     /gene="arsM"
                     /locus_tag="PLU99_08430"
     CDS             <9532..10244
                     /gene="arsM"
                     /locus_tag="PLU99_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOO17122.1"
                     /translation="LPEGANMGLSCGNPTAIAALKPGQVVLDLGSGGGFDVFLAGPKV
                     GAAGRVIGVDMTPEMLTKARRNIAAYTQNTGLENVEFRLGEIEHLPVADASVDVVISN
                     CVINLSPDKPQVWREIARVLRPGGRVAVSDMALLRPLPPEISGLFEALIGCVAGAVLL
                     DEYRQMMLAAGLAEVVIRPKPEYVAALTSFKDPLYRRIEEALPAGTTVGDFVTSIDVT
                     ATKPLAGSDVTEPLPAVM"
     gene            10446..>11074
                     /locus_tag="PLU99_08435"
     CDS             10446..>11074
                     /locus_tag="PLU99_08435"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOO17123.1"
                     /translation="MMTKRARLSSMGPVVAALAAVFMVTGSALGHCDTMDGPVVKDAQ
                     AALVKGDVTSLLKWVPQANEDEIRQAFQKTLLVRAKGPEAQELADMYFFETLVRVHRA
                     GEGAPYTGIKPAGTPLPAPIAMSDAALEQGNLDELARHIAAAVETNIRERFAAAAEAR
                     KHKDESIESGRKFVAAYVEFVHYVEAAHNLATAHGAHHAQGGCVQHAAAP"
BASE COUNT         2106 a         3683 c         3369 g         1816 t
ORIGIN      
        1 cgcttgtgaa gacgcgtttt ccatcgactt ccacgtggat tgttgcacta cccgctccgc
       61 cggtgccgtg gtctatgccg gccagggcac gaaatctggt cgcgttcggc ggcacggcat
      121 attcgatccg cgagggcgcg tgcgttccga gaccgcgcaa atagatcgtg ccgccaagct
      181 ccagcggacc gcccgcgacg gcccgatcca gcgccagcga acgccacgcc tgttgcgcgc
      241 gcaccggctt catttcgctc aaatacaccg catcggtcgc ggcagccact tccggcgccc
      301 tattcgccaa caccgcgatc gaagtcgcgc cggccaccgc aatcaccacc gcccccagcg
      361 tggcgcgccg gaaccagacg atcagcggct gcgatgtcga cagcgacggc tcgtcagcaa
      421 tttcctttgg cggcaacgcc ggtgcgcgca gcggcaggga aggttcgcga accgcttgcg
      481 gccaggcgag cgccaccagg agcacgccaa aaatcaacag gctcgccgta gcgatctgcg
      541 gggcaagccc gcctggtggc agaacgaaag ttaaattcat caagagcagt gccgaaagca
      601 ggaagtaagc ccgctctctc cattggcccg acaccgccca cagtgccagg aaggccagcg
      661 cgggaaacgc gtaacgctcg tgcatttcgg tcgggaacag gaagaacgcc aggccgagca
      721 gtgcggctgc agcgtagcgg gcgattggtc cggggcgcag tgagtagatc gccagcacaa
      781 ctgccacgct cagcgcgtac accagcagcg aaatccgtcg ccaagtcagt gccagcaacc
      841 acgatgagcc ggcgggtatc tcagtctgcc ccgcggccgc aatgcgtgtc agcacgaccg
      901 gggcggccgc gtcggtcgtg tcagcgggcg ccaccaatgt ccacgtatta aaggctccct
      961 tcgaaagctg cttgtactgt ccgatcacgt tgacatagcc gcgctgaagc acctcgccga
     1021 gcgtgcccgt cagcgtgaac ggcgtggtta ccacggccgc cagggccacg gcggcgacga
     1081 gcttgccggc aatcgcgcgc cagccgccca ggcggtaggt ctcgaagagc agcagcggca
     1141 cgaacgcgat tgtctggaac ttggcgagca aaccggccgc caccgctgca cccgccaggt
     1201 accaatgccg ccgggccatg agaaccacgg cgagcaacgc gaaaaatgtg taaatggcgt
     1261 cgacttgtcc ccagtaggct gagttgtaga gcgccaccgg gttcaaaaag tacagtgccg
     1321 cggcccccaa accggctcga ctgcccagca gccggcgggc ttccacgaat agcaagagcg
     1381 cgattgccag atcaccgaga caggcgggta ttttgaggat gatgtgcagc aactcttcgg
     1441 aagctgagcc cccgccgatt gccgcagtgc tcttgcccac cgcgtaaaag acgagcaccc
     1501 ccagcggcgg gtagtcgcag aactgttggg tagcatagaa ccttgccaac ccatcgcttg
     1561 cgagatgacg cgaccaatga ctgaagatgc gcagatcgtc ttgcagcccc agtcccggcg
     1621 tggcgacgat ccatatccgc atcgccagcc cgaccagcag aatgccggcc aatgcggccg
     1681 cgacaaccca aggccgtcgg cgatcgccgt gctgcgagcc gctgcctggc tcgggtgata
     1741 tgtcggaaac tatcgccgaa tcggctaagg ggcccatcgc agccgccggc tccagttcct
     1801 ccggtttggt cgaattcatc acggcgctcc tggcgtgatt gcttctttcc cgggctcctc
     1861 gccggcgttg gccgccggct gcgcagcctg actggccgta ccaggtttgt gcatgaagcg
     1921 cgcatccgcc caatcgacgt ggtcgaagcg ttgaccatcg gcagtggtct cggccaccag
     1981 tagtatctgt cgcgcgccgg ccagcggaat ccgcacgaca gccggctcgc tgtcgcccgt
     2041 cagcaccggg ctttcgaaag cccgcgtgcc gtcaagatac accgcggcta ggatgctgcc
     2101 ccggccttca gtttcgtcat ccacgcccac cacggcctca aaccagtcgt agccggcggg
     2161 cacgttgtac agcagtttgg acggggcatg cgtgccgatg ccgcggcggt aagtagttcc
     2221 gcgcaggcgc agccggcgat tctcaacact ggcatcgctc gtcagctcgc cccagccctg
     2281 atcggccgat actggttcca gcgcactgag gtacacgtga ccttccaagt ccggagccgg
     2341 cgccgtggcg ggagcagccg gtggctcatc cgcgtcgcgg atgcggaaca ccaggaacgg
     2401 tgcattgcgg caagcggcgt atgtcttgca agggtagacc agctcggcca gattgcggtc
     2461 gcgggccagc ttgctcgcaa agctctgatg atcgtcatcg cagatcacga agcgggcttt
     2521 aaactcggtg cgaatgtctt tcagatcaat gctgatagtc tcctgccgcg tctggccgtc
     2581 ggggccggcc agcttgacta cagactgctt cggcgtctca ccgcgggtga tgcgcatata
     2641 gcgttcccac aaatcggggc gacgcgcgtg tgtgaacttc ggatcgaggc cgacgacgta
     2701 gtaattgtgc gagttgtggt aaaagtaggc gggaaatatg tcccagtcat cagtaaagac
     2761 aatatcgcca ggattggaat tctcttcgac gaacttcagc gccgcgcgca tcgccggcaa
     2821 gttgaagccg gtgcgcatct gctcgcccac cgacctgatc tgacgcgacc cgaccgacag
     2881 agtcgcgccg aacagtccca gtccaaccag aacaatcgcg gccgccggga cgagcaccgg
     2941 agcgggttgc gacgcttcca tcggccggcg cgtcaggtga taagcggctg gggcgatcac
     3001 gtacagcgcc gccacgacaa accacaaggg gcgcgcatca agccgcgccg ccggcaggtt
     3061 ctgcacgccg aacctaaaat gcactaacaa tgccaggcag ccgaacacca ccgccgccgt
     3121 agccggcagc gccgccaggc gcaagaccgg aacctgaccg cgctcgcccc gctgcgccgt
     3181 ccagacctgc gtcagcgccg gcaacagcag cagcgccagc atcgcggccc agcttcgcca
     3241 atcagccagc acagccgggg cgtccggccg ctcactcccc ttccacagca aataaccgat
     3301 gcacgcggcc accaggccca gaatcggcca ggcggtccaa tttctgtgca gcgtctcccc
     3361 cggccgcagc gtgggaatga gcgccggcaa gcccgccagc acgggggcgc tcagataagc
     3421 cgcactgagc acgcagaacg gcggccagta ttcgataaaa cgccgcgcct tgaacgtcag
     3481 cagcagaaac gcgaaattga tcagcagcag cgccagctcc tgggcattca gccgcggccc
     3541 gcgtcgcatc cgcaggcata ccgcgcccgc ccacaccacc gccggcacgg ccgacatcga
     3601 caggaaccac cacgtattca cgtaggggtt ccattcctgc cccacctcaa tatccggcgc
     3661 caaaccggtc ttagtgacct gcaaaatcag aaattccagc atgccgctga agtacgggta
     3721 gctcacgacc cccagggccc acccgcccgc cgtccaggct accatgcgcc atgggaagcg
     3781 ccgatcgtcg gccggcccca atacctgcgc cagcgcatag agccccacca gcagcggtcc
     3841 gaacatgacc gcgcccaagt agagctgcac gtacagagcg gcgacgaggg ccgcccacac
     3901 gtaccgcccc ttgaagagta gcagcagcaa aagctgcatg aacaagagcg acgccccgat
     3961 tgcccgcacc agcgaatgcc gcatgaagaa ctggtccggc atcagcagga aaatcacgag
     4021 ccaaccccag cgatacggca cgttcccggt gcgcagcaac aggttgaaca gaacgaggtt
     4081 gaggccgaag aacgtcgccg ccgcccagcg tccgccggcc agaaaatcgc ccgtcagcca
     4141 ttgcgacaag tacacgaacg gcatcagcag cacgtgaaat ccgaaatggt ggctgacgaa
     4201 ttcgtgccct tcctggcgga aatacgcgta ctgcagccac ggcagatcgt ggacaattcc
     4261 gacctgcggc agcagggcgg ccatctttat gtggtagtac gaatcgtgcc cgggtactcc
     4321 gagttcgtcg cccgcgccgc gcgcggagct gtggtagaag taggtcatca gtgcaatccc
     4381 gaacacgagc agcgccaggg attcgatgat gaccaggacg cgcgaactcg gatcacgccc
     4441 tgtttcgact gtgcctgcca ttctccgcct tgctcattcg gcccggcaag ccccccggcc
     4501 cctccattct agcagagccg agccgttccg tgttgagcgg cgttggcgta cggcgccggt
     4561 tgctctatcc gcgccgctta cgttattctc gatccggtag cccagcttca ccggaatggt
     4621 gccagcgtgc cccgtaaagc ttctcagtct gcgacagccg aaattccgac ctgcccgacc
     4681 cccggcgcgg agaaaccggc aacggcgccc acggcgcgct ttcgccacct gctgctactc
     4741 atcctggcag tgggcgccgc ttgccgcgcg gtcatcctcg tcgattatct cgcgcataac
     4801 ccgaccgccg ccacccctat caccgacgcc gcctggtact ggaactgggc cgaacggatc
     4861 gcggccggcg agctgatcgg cagaatgccc tttacctcgg cgccgcttta tccgtatctg
     4921 ttaggcttgc tacgcgcgct gggtggcgga ctcaagctac tctatttggc ccagaccgcc
     4981 gtcagcttgc ttactgcctt cctgctggcc gaaatcggcc ggcgacgctt cggcgaagcg
     5041 gtgggcttgt tggccgcggc gctattcctg cttctgcaag aaccggcctc ggccgcactg
     5101 cgcgtcctgg ccagcacgct cgaattgctg acggtagtga tcgtctgggg cctgctggtc
     5161 cgcttgcaag cgcggccttc atcatgggcg gcgcatgtgg cagccggcac cgggctcggg
     5221 ctgctgtgcc tggcgttcgc ccccgcgctg ctgctattgc cggcagcggt ttgctggctc
     5281 gcgctccgca cgccgcgttt gccggctatt gcccgcgcgg ctgctttcgc cgcggccgcc
     5341 gccgtcgtca ttgccccggc aacattgcac aattggctgg gcgcgaacga gttttgcctg
     5401 atccggacag gcgccgggct caccttgcgc caggggaacc acccgcaatc cgtcggcgcc
     5461 tacaccccga tccccggcgt aagcgtgcag cgcgaaaagc agtacgagtc ggccgccgag
     5521 gtctaccggc aggaaacggg acagcagccc acctgggcgg ccgtggacgg ctacttccgg
     5581 cagcaagctg tgtcattctg gcgcgcaaat cccgggcggg ccctggagct ggcagcggcg
     5641 aaactgtacg ccacgctgac ataccagaac tacagcgaca tttaccaaac gcgggccgaa
     5701 attgcgtgcg gtttgacacg cgctttgtgg ctggcaccct tgccaatgcc gctgctcatc
     5761 gccgccgcgc tgatcggagc actgtactgg ctgcgccgcc cgcttttcta cgcgccagaa
     5821 tggatgcttc ttattctccc cattctggtg actgtgttgt tcttctattc accgcgctac
     5881 cgcttgcccg cggcgcctct actcaccgtg gctgcggcgc tcgcgctggt gcgcgcctgg
     5941 cattggcgcc gccaccgggc cacgggtgcg gccgttgtcg gcgcgctggc cgcaagcctg
     6001 acgctgaccg cggtcaacgc ggcctgcggg cgcgacgcgc tggactacgc cggcgtctac
     6061 cgcgcgctgg ccgaagccta tgcgcagcag gaccggatgg acgattcaac ggccatgctt
     6121 cgccaggccg tacaggccga ccccggcgac gtggatagcc gcatctgtct ggccgaccgc
     6181 ctgcgctcct tggaccgcgt ggacgaagct gcgagcgagt atttgcaggc ctttcaactc
     6241 gcgcccgacg atgcagcgct cttcagccgc accttcagcg cgctggcgcg agcgcagcgt
     6301 ctggtcgagg cggaagcact ggcggcccgc gtaatgcagc gccatcccgc cgatccggtc
     6361 tggctcgcgg ccctggcgga agcgaaactc ggagcaggcg acttgcgcgg cgcgaacgag
     6421 tgcatcgcgc aggctctggc gcttgcccca gaaaacacgc gctggcgcca cctgcaggtc
     6481 gatgttctca tcgggctgca gcgcttcgct gaagccgaac gcgaactgga acgaattctg
     6541 aagtccgccc cggacgattg cggcgcgctg tggcgacaag gccaaatcgc cgggcgaacc
     6601 gggcggcccg cagaggcacg gcggctgctc gagcacgccc tcgccgtctg ccctgataac
     6661 gcgccggcct ggcacgatct ggcggtcgta aacatgcaga tgaaccggcc gcaggcggcg
     6721 cgggattgcc tgcgcaaagc gctgcaagtt aacccgcgcc aggagctctc gcggcaactt
     6781 ctggctgagc ttgatagcct gggcacgccg cccgttacgc agccgtagca ctgcaccagc
     6841 gactgactcg ccggtcatcc gagcggcgtc ccgcgccttc ctagaatcag gcgggctgcg
     6901 gacgggctcg cggagtggct cgcggcgaac cggcccactt cttccgtcca gtgtgggtcg
     6961 cccatgattc aacatacaca tgcgcaaacg gcccaaaccg ctgacgtact ccacggggta
     7021 cgccagcacg tgctgtggct tctggacggc ggcaacgctc acctggattt cgatgccgcg
     7081 gtggccgggc tgccggccaa actgcgcggc gctaaaccca tcaatgtgcc gtattcgccc
     7141 tggcggctga tcgaacacat gcgtctgtcg cagtgggaca ttttggaatt ctgccgcaat
     7201 ccgaagcacg tctcgcccga gtggccggaa ggctattggc cgcagtccga cgcgccgcct
     7261 gacgaacggg cctgggaggc cagcctgcgc gaatttcacg acgatctggc cgcgatgcgg
     7321 gcgttgatca aaaaccccca ggccaatttg cttgcaccac tgccgcacgg cgagggtcag
     7381 acgctcttgc gcgaggccct gctgctggcc gatcacaatg cttatcacct gggacaactc
     7441 gtgaccgtcc gccgggctct gggcgcttgg cccgaagaat agccgaccgc agaagcacgc
     7501 gtcagcacac gcggcgctga gcgccgtcgc cgttgacacc ggctggcacg taatcgcatt
     7561 tcgaaaacgc cgcgccgagg cgcgcccacg cttggccggc acgcgctgcc gcgtcgcgcc
     7621 cgcgtgccgg actcgcaacc gagtgttgac cttcccgcgt attacgcgaa actacacagc
     7681 acgtgtccga aggacgcaaa aacacggggg ttataaacgg gggcggtgag gatcattacc
     7741 agagacatga acgagtctga tgcacgaaag gaacacaaac cgaagatttt ctctgctgat
     7801 ccaatcaaac aagcgggccc ggccccctgg tcggcccggc ggcggcgccc gaacctgctg
     7861 gtgctcggag catcggggca tgtggccggg gcgtttctgc gacgcttggg cggacgccga
     7921 gatctcttta atgaagtagt ccttctcgac cgcaacgagc aggtcgtgga gaaccggttt
     7981 ctggagcatt cgcgcctgcg gtacaccttt gtacaccatg atcttcggtt cagagatcat
     8041 gacacggact atgtgcggtt actgcaacaa cgccagattg acatcgtcct cgacctgacg
     8101 gatctggaca cgatgccggt gtttcaagcc acggatgccg ccggcgtccg ctatatcaac
     8161 accgcgctga atgatgccaa cgccggggtg cacgagatgc tggccagaat ccatccggtc
     8221 cggcaacggc cgcgacgtgc gccgcacatc atcagcagcg gcatgaatcc cggggcggta
     8281 aacatttggg tgatgcacgg cgtgcggaat tacggcgtgc cgcgcgagat catccatttt
     8341 gagtacgaca gttccatgtc ctttgaccgc tggcgtccgc tcatcacctg gtcgcgccgc
     8401 gagttcctca ccgagaccgt ctgggagccg accggccagg tcgaccatgg ccgggtgcga
     8461 gtcttcgaca gcaacgcact ggcaaaccgc gtgaatctgg ggccgatcat ggaaccggtc
     8521 cataagctgc catcttaccc gcgcggcttt ctcgtcctgc acgaggagaa tatcaagctg
     8581 gcccagaagt tcggcatctc ctcgaaatac atctatgccc tgcaccccaa gaccatggac
     8641 ttcctggtgc gacgctggcg tgagcacggc accgtcctca tcagcgatct cgaagtaggc
     8701 gataacacct ccattccctt gaccggcagc gatacgatcg gcgtctgcct gcagtatccg
     8761 cgaaagcgcg tctattacgt gcacagcctg tcgaaccagt cggtcgtggg cacaaacgca
     8821 acttgcgcac aggtcgccat cggcgtttgg gcggccctga cgaccatgct caccgaacca
     8881 ctccagtcgc gcgtctattt cccaaccgat ctatacggca cgatctatcc gcacgttttg
     8941 ttctcaaacc agcgcgtcga agtattcgag tgcggctggt acaacaagac ctggaccgtg
     9001 ctgaaacaca ttcccgaatt gcacccgcgc ctttcgcgcg gcaaggagca gtcgatcatc
     9061 taggagattt gcttcattga cccgcgacgg agcgcggcta taattcgtta gttggcgatg
     9121 tgagaaatcg acccgtgaaa tcgtttttgg ccattacgag agccctgtcc gacgaaaccc
     9181 gcctgcgggc actgctggcc ctgcggcaag gtgaactgtg cctgtgccag atcatcagcc
     9241 tgctgggcat ggcgccttcc accgtttcca aacacatgga cctgctttac caggccggac
     9301 tggtcgagcg ccgcaagcag ggaaaatggc actactttcg gctcgcggga cgagaagcgc
     9361 ccgccgcggc acaaaaggcg attcgctggg ccttggatgc cttggcgaac gagcctctgg
     9421 tgaaggccga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natctgcccg
     9541 aaggggccaa catgggcctt tcgtgcggca atcccacggc catcgccgcc ctcaaacccg
     9601 gccaagtcgt cttggacctg ggttcgggcg gcggctttga cgtgtttctg gcggggccga
     9661 aggtgggtgc tgccggccgc gtcattggtg tggacatgac gccggaaatg ctcaccaagg
     9721 cccggcgcaa catagccgcc tacacccaaa acaccggcct ggagaacgtg gagttccgcc
     9781 tgggcgagat cgagcacctg cccgtagccg acgccagcgt ggacgtggtc atttccaact
     9841 gtgtgatcaa tctttcgccc gacaagccgc aggtgtggcg cgagatcgcc cgcgtgcttc
     9901 ggccgggcgg ccgcgtagcc gtgtccgaca tggccctgct gcgtccgctg ccgccggaga
     9961 tcagcgggct attcgaagcc ttaataggct gtgtggccgg ggcggtgctt cttgatgagt
    10021 accggcaaat gatgcttgcg gccggattgg cggaggtcgt cattcggccc aagcctgaat
    10081 acgttgcggc gctgacgagc ttcaaagacc cgctctaccg caggattgaa gaagcgcttc
    10141 ctgctggtac gacggttggt gatttcgtca ccagcatcga cgtgactgca accaaaccgc
    10201 tcgccggcag tgatgtgacc gagccgctcc cagcagtgat gtgaccaagc cgttgaacgg
    10261 cagcacgtga cggcaaccaa gcggcccacc ggcaccgatg tgactgcggc caggccgtga
    10321 ccctcggagc cgcccatggt tctcggcgcg aagatttcga gtgcacagca cgccacgctt
    10381 cgacaggcaa tggcaagaca acactcaaag ggcaatcgga aaaccgaacg gaaaggaacg
    10441 aaagcatgat gaccaagaga gcaagactga gcagtatggg acccgtcgtg gccgcgctgg
    10501 ccgcagtgtt catggtgacc ggctcagccc tgggccactg cgacacgatg gacggaccgg
    10561 tggtcaagga tgcccaggcc gccttggtga agggcgacgt aacgagcctc ctcaaatggg
    10621 taccccaggc gaacgaggac gagattcgcc aggccttcca gaaaacgctg cttgtccgcg
    10681 ccaaggggcc tgaggcgcag gaactggccg acatgtactt cttcgaaacg ctggtgcgcg
    10741 tgcatcgcgc cggcgagggc gccccgtaca ccggcatcaa gccggccggc acgccactgc
    10801 ccgccccaat cgcaatgtcg gacgcggcct tggagcaggg caacctggat gaactggccc
    10861 gccatatcgc cgcggccgtc gaaacgaaca ttcgcgaacg cttcgccgcc gccgccgagg
    10921 ccagaaagca caaggacgaa agcatcgagt ccggccgcaa gttcgtcgcc gcctacgtgg
    10981 agttcgtcca ctacgtcgaa gccgcccaca acctggccac cgcccacggc gctcatcacg
    11041 cccaaggagg atgcgtgcag cacgccgccg ctcc
//
LOCUS       DAOSYS010000113        11047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5050_length_11047_cov_5.845069, whole genome shotgun sequence.
ACCESSION   DAOSYS010000113 DAOSYS010000000
VERSION     DAOSYS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_11047_cov_5.845069"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1484
                     /locus_tag="PLU99_08440"
     CDS             264..1484
                     /locus_tag="PLU99_08440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17124.1"
                     /translation="MQILAIKRAAPGGKANGLDLPVREILGRLAARHDVTLASWGGQA
                     PAPRELNFRTTVRPLPAANAQTRGNSGNRRELRLLDHYGFAAETVPWLASLMADYRPD
                     VVIGIDYATLPALAGNRAVPRLAYLLDNRFLSARQELRTGNRSPAAALKELVWSWRLH
                     RRYGRSADAFIFVTEQEAAGFRRFTRRPCWVITNGVDVERFRPSGAARDTNTVVFVGS
                     LDFPPNIEATAWFCEQVWPHVRRACPDARFQIVGRDPVPRVAALAGREQIEVISDPPD
                     IRPYLARAAVAVAPMRSGGGVKNKILEGWAMGTPMVVTRMSLAGLAARPGDNILAADR
                     PADFAANLVLCLRDTSLRDLLAARGRETVLAEHTWEKAAAQFEECLAAVINVDECQLP
                     EAKSDCRFATFINR"
     gene            1518..2336
                     /locus_tag="PLU99_08445"
     CDS             1518..2336
                     /locus_tag="PLU99_08445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO17125.1"
                     /translation="MARDLSGKVIVITGASAGIGAATAIECARAGMDVVLNARRAERL
                     EAVAADVRSLGRTAEVVPGDITERGFENRLLDAARQRFGGFYAVLANAGYAINRPIHE
                     MSEQELRQIFEVNFFAATWLLAEAARRLITAGRRGHLLMTSSAVAKFTFPNTGAYSAT
                     KAAQNHVCRQMRLELRPYGIAVSSVHPITTRTEFFDVAAQKTGRQPPVKNGFEHAPRF
                     LVQSPEVVARAVVRCLRHPVPEVWTSRFVRTVAGVVTAVPGLADQLASRRNRGQ"
     gene            complement(2358..2591)
                     /locus_tag="PLU99_08450"
     CDS             complement(2358..2591)
                     /locus_tag="PLU99_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17126.1"
                     /translation="MPCAEQNLDAIAVPTGVNGVYVVPLSALDFENPPPLLRGRPITA
                     IAPHPQHDDRYYVTTEHPDALAFLPDPYIDLLP"
     gene            complement(2638..3165)
                     /locus_tag="PLU99_08455"
     CDS             complement(2638..3165)
                     /locus_tag="PLU99_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17127.1"
                     /translation="MPNQRVTEFFGPDWGRGPFSSYRIETAALQIATALAQLRVPLLR
                     DDEQIKSHAEFIRQLADCLAPDWAVQISSDVGQLSGGAIPTSILAATGTFSLLDCWLA
                     DSPGGGLTSTAPSSVAWTSGTVLQTIVERKRYLIVTPSTGVATVNVSYGGVKNWYWAI
                     CRQGRVYYSSQLSFF"
     gene            complement(3608..5083)
                     /locus_tag="PLU99_08460"
     CDS             complement(3608..5083)
                     /locus_tag="PLU99_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO17128.1"
                     /translation="MWEAIASNRRRSWVLIGTMGGVLVLLGAVIGMSVAAQLDGGWSR
                     AHPSYYYVPEAPVVSPVVSMEAAAAIGVAVAVVIWLVLWAIAVGAGDSIILKSAGARE
                     ITKEQAPQLWNVVEEMSIAAGLPAMPRVYIIDDSSLNAFAVGRRPEKAAVAVTAGLLK
                     RLNRDELQGVIGHEIGHIHNLDARFMTLAAVMLGAVVLLSRGFLRGMFYGSGGRRSSR
                     GGGQAQLIILLAAALVAVLAPLAAQLLYLACSRRREFLADASSARFTRYPEGLASALE
                     KISRQAGGQAEKNRVLAPLYIVNPLQEHAAFSLFSTHPKSSERIAILRRMGNAGYAAY
                     EEAYRKIRGGACIGAGTLAKDEGASLRAPTAEPEPRASAVERAEAVGRLLDTVLPLAV
                     IQCACGARLKIPGNFKREQVACPRCGREHATPRAAASASADAASRSMTYVRKGQGWES
                     FDCACGHLIQLSPGFGAESVKCPKCRRKIAVTAKGESCVGP"
     assembly_gap    5104..5203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5204..5652)
                     /locus_tag="PLU99_08465"
     CDS             complement(<5204..5652)
                     /locus_tag="PLU99_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOO17129.1"
                     /translation="MMIVLLVVVAVLLLAFLWVIGTYNGLVRLRNQVKNAWAQIDVQL
                     KRRHDLIPNLVETVKGYAKHERGTLDAVVQARARATGAQTVDDRIRSEGELSQALGRL
                     MVVVEAYPDLKANQNFLALQEELTSTENKISFARQFYNDSVMQYNTR"
     gene            complement(5661..6086)
                     /locus_tag="PLU99_08470"
     CDS             complement(5661..6086)
                     /locus_tag="PLU99_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOO17130.1"
                     /translation="MCPACNKPMIVVEFDGVEVDYCADCHGVWLDTGELELISELAGG
                     RTNPLEAPVADPLEAAVRDPGPTSRDKRRCPRCRRRLRVQPVGTRPPVEVDRCPAGHG
                     LWFDAGELATVVGGGEPNAPVAEFLGQLFRQNTSQTEKS"
     gene            complement(6495..6860)
                     /locus_tag="PLU99_08475"
     CDS             complement(6495..6860)
                     /locus_tag="PLU99_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO17131.1"
                     /translation="MAGRLEQFRTYREKMNERILAAGNLGINRFFALDGKAYADGALD
                     GKTKELAGLAASLVLRCDDCIAYHVIRCKELGVTNEQLFELLNVGLIVGGSIVIPHLR
                     RAVELLDELDAGAATGEAE"
     gene            7059..7304
                     /locus_tag="PLU99_08480"
     CDS             7059..7304
                     /locus_tag="PLU99_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17132.1"
                     /translation="MADEASELCVELSRQHPGAVIFAGTLVFEHPHWYHRVLHNETAY
                     AIPRRLKFAGIPVVILPLLLRRPKHAGQPPATLPSSP"
     gene            complement(7746..>8390)
                     /locus_tag="PLU99_08485"
     CDS             complement(7746..>8390)
                     /locus_tag="PLU99_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOO17133.1"
                     /translation="ALLGYERAIVSPVLGTTRDVLSAEICLDGVQLVLQDCAGLGRTA
                     DDLTAAAHLAAERNAAQADVLVWVQAWDVPWDAGMAAVLDVVPPDRRLVVLSKSDLAG
                     GDRPAPVPASVLGRHVAVSAARGEGMAELRRQIAECVCRVAMPPAVCDGTLAAATAAL
                     ERARGLCEGAGVPEQLELISLEVRSAYEGLEGRPHAELVDNLLGRLFSEFCIGK"
     assembly_gap    8391..8490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8491..8936)
                     /locus_tag="PLU99_08490"
     CDS             complement(<8491..8936)
                     /locus_tag="PLU99_08490"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOO17134.1"
                     /translation="MLFPAINDTIIAVSSGWQASPLGIVRLSGPGAWEYAASVGLRLP
                     MAGADGGRGPFITENGRITAGQLEFPGTACWFKAPRSYTGQDLVELHVPGCLPLLREL
                     CARLIERGARRALPGEFTARAYLAGKLSVEQVEDVLATISADRDSE"
     gene            complement(8946..9506)
                     /locus_tag="PLU99_08495"
     CDS             complement(8946..9506)
                     /locus_tag="PLU99_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17135.1"
                     /translation="MLVGAGLLPLAAMVWLACRPSGAQHSTAATGQEAVAIPIVLSGP
                     EQAARSALLCLKAIHEARASRDEEAVSRYLEQLDLVAARDTIRRRFQTSYPHSAKDPE
                     AAVDNFVECWAAVVGYYADGLDPNRVNAVDAAAGAFQVDVHATAAGSRGEATIRFECL
                     RGDDGNWRVSRVDFAPRPAPSTQPSP"
     gene            complement(9603..>11047)
                     /gene="metG"
                     /locus_tag="PLU99_08500"
     CDS             complement(9603..>11047)
                     /gene="metG"
                     /locus_tag="PLU99_08500"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOO17136.1"
                     /translation="DFAGLGIEFDVYGGTHQPGYREMHERISQEFFLRIHEKGYFTKK
                     STRQLYDPKAARFLPDRYVRGKCHHCGAAGATGDQCDNCGQMIDPLLLINPVSEITGE
                     RAEVRETTHWFLSLQKFEQPLKEWLESKRGQWRANVLNFALGQIKQGLPERSMTRDIS
                     WGIPVPLDDPDARGKVLFVWFDAPIGYISFTAQLFADQLGNWREYEKWWCDPESAIVH
                     FIGEDNTVFHALIWPAMLMADGRHQLPSAVVANNFVNIRMGEGDEKVSKSKTAENSPV
                     WIQEYLKRFDPDALRYYLTAIAPETARTSFDPADFLQRNNSELVAAFGNFVNRTLTFP
                     ARYFDGKMPAATAQTPRDREHLAAGEAALTKVGQQIEGHHFRAALEEMMGYARLCNEF
                     FSIREPWRTRKDDPADCAAAIATCIHAVQYLATMAYPFMPAAAKRLLTMLNAPSEQIR
                     WEPPVPLPEGHVLGQPAILFQKLEPDAMSM"
BASE COUNT         1943 a         3794 c         3203 g         1907 t
ORIGIN      
        1 gcgacaatcc cacaattata aggccatccc cgaccgcggt caccacgccg taacccgtca
       61 cgcgctaccg tgtcggttgg tacaggcgtt ggagggtgca cgggtcgggt gccataccca
      121 agccgcgtag cggccgggca tgcctccgac gaatcgcctc acgcgccgga ccggccagct
      181 tcaaggtgca gccctgcggc tccgataagt ccagcagcgc agcgcccatc gcgaaggccg
      241 cccgcgcaat tggccggagt cccatgcaaa tcctcgcaat caagcgcgcc gcacccggtg
      301 gcaaggccaa tggcctggac ctgccggtgc gggaaatcct gggccggctg gcggcgcgac
      361 acgacgtcac tctggcaagt tgggggggcc aagcccccgc gccccgtgag ctcaactttc
      421 gaaccactgt gcgcccgctg cccgctgcaa acgcccagac gcgcggcaac tccgggaacc
      481 ggcgcgaact acggctgctg gaccattacg gtttcgccgc tgagaccgtg ccctggctgg
      541 catcgctaat ggcggattac cggccggacg tcgttattgg catcgactac gccaccttgc
      601 cggccctggc cggaaaccgg gccgtgccgc gcctggctta cctgctcgac aaccgtttcc
      661 tctccgcgcg ccaggagctt cgcaccggca atcgctcgcc tgccgcggcc ctcaaggaac
      721 tcgtctggtc gtggcgcctg caccggcgct atggccgcag cgctgacgcc ttcattttcg
      781 tgaccgaaca agaagccgcc ggtttcaggc gcttcacgcg gcggccttgc tgggtgatta
      841 cgaacggcgt ggacgtcgag cgcttccgac ccagcggggc ggcacgcgac acaaacaccg
      901 tcgtcttcgt cggcagcctc gatttcccgc ccaacatcga ggcgaccgca tggttctgcg
      961 agcaagtctg gccgcacgtg cgtcgcgcct gcccggacgc gaggtttcaa atcgtcggcc
     1021 gcgatcctgt accgcgagtc gcggcgctcg ccggacgcga gcagatcgag gtgatctccg
     1081 atccgccgga tattcggccg tacctggcgc gggcggccgt cgcagttgcg ccgatgcgca
     1141 gcgggggcgg agtgaagaac aagattctgg aaggctgggc catgggaacg ccaatggtcg
     1201 tcacgcgcat gtcgctggcg ggcctggcag cgcggcccgg cgacaacatt ctggcggcgg
     1261 accggccggc ggatttcgcc gccaacctcg tgctctgcct gcgcgacacg agcttgcggg
     1321 accttctagc cgcccgcggg cgggaaaccg tgttagccga gcacacgtgg gaaaaggccg
     1381 cggcccagtt cgaagagtgc ctcgcggcgg tgatcaacgt tgacgaatgc caactgcccg
     1441 aagccaaaag cgattgccgc tttgcgacgt tcataaaccg gtagcggatc gcaacaatca
     1501 ggtctaaagt ctcgcgcatg gcgcgtgatt tgtccggcaa agtaatcgtc atcaccgggg
     1561 ccagcgcagg catcggcgcg gcgaccgcga tcgagtgcgc tcgcgccggc atggacgtag
     1621 tgttgaacgc ccgccgggcg gagcggctgg aagccgtcgc ggccgacgtc cgcagtctgg
     1681 ggcggacggc cgaagttgtg ccgggcgaca ttacggagcg cggattcgaa aaccgcctgc
     1741 tggatgccgc gcggcaacgg ttcggcggtt tttacgcggt tctggccaac gccggctacg
     1801 cgatcaaccg ccccatccac gagatgagcg agcaggagct gcgccagatt ttcgaggtga
     1861 atttcttcgc cgcaacgtgg ctgctggccg aggcggcgcg gcggctgatc acggccggcc
     1921 ggcggggaca tctgctcatg acgtccagcg cggtggccaa gttcaccttt cccaacaccg
     1981 gcgcttacag cgcgaccaag gccgcccaga accacgtttg ccggcaaatg cgcctggaac
     2041 tgcggcccta tggcatcgcc gtctcatcag tccacccgat cacgacgcga acggaattct
     2101 tcgacgtggc cgcccaaaag accgggcggc agccgccagt taaaaacggt tttgagcacg
     2161 caccgcggtt tctcgtgcaa agcccggagg tggtggcgcg ggcggtggtt cgttgcctgc
     2221 ggcatcccgt gcccgaagtc tggacgagcc ggttcgtgcg aaccgtggcc ggcgtcgtga
     2281 cggccgttcc gggcctggcg gaccagttgg ccagccgtcg caatcgcggg cagtgaaaag
     2341 cggcccgaga cttcgcatca cggcagcagg tcgatatacg gatcgggcag aaaagccagc
     2401 gcgtccgggt gctcggtcgt gacgtaatag cggtcgtcgt gctgcgggtg cggcgcgatc
     2461 gcagtgatcg gacgcccgcg cagcagcggc ggcgggtttt caaaatccag cgccgacagc
     2521 ggcacgacat atacaccgtt tacacccgtc ggcactgcga ttgcgtcaag attctgctca
     2581 gcgcagggca tactcacctc actgaaactt gcatagacaa tccgccgccg gcctacatca
     2641 gaagaagctc agttgcgacg aatagtaaac gcggccctgc cggcaaattg cccagtacca
     2701 attcttaacg cccccgtagc tgacgttgac ggtcgccacg cccgtggagg gtgtgacgat
     2761 gaggtagcgc ttccgttcga cgatggtctg gagcacggtg ccggacgtcc acgcgaccga
     2821 actgggcgcg gtactggtga gcccgccgcc cggactgtcg gccagccagc agtcgagcag
     2881 gctgaaggtg cccgtagccg ccaggatgga ggtggggatg gcgccgccgg aaagctggcc
     2941 gacgtcactg ctgatctgaa cggcccaatc gggcgccagg cagtcggcga gctggcggat
     3001 gaattcggcg tgcgacttga tctgctcatc gtcccgcagc agcgggacgc gcaactgcgc
     3061 cagggcggtg gcgatctgca gggcggcggt ttcgatccgg tagctggaaa acggaccgcg
     3121 gccccaatcg gggccgaaaa actcagtgac gcgctggttg ggcatggcaa ctctccctgc
     3181 aatctgtgcc cggcggcccg ggctgccgcg cccggccggc cgcctgatct ttatagccat
     3241 tctagaacac atagtcaagc agcacgccga actttctgtg ctcccaacat gtgccgccag
     3301 ccacagcggg cctgacggcg attcttgcgt cgcgaggcgc caacccgtcg ttcgcccccg
     3361 gccaacttgg tttgcgtggt tagaatgtca gcttgctgtc gctatgaagt agatccagac
     3421 gtagctatat atgaaagagc gaatcgccat cgaactgccc ctgacgcgca ttgccgattt
     3481 ctgtcgccgg catcacatcc gccgtgcaca cgccggggtt cttgagcaag tacttccgcg
     3541 aggatgtttt gcgccatgct gcggtcgttt atgacgctgc atagcgatga tatgaattac
     3601 ggacacctca cggaccgacg cacgactctc ctttagccgt caccgcgatc ttgcgacgac
     3661 atttcgggca cttgacgctt tccgccccaa accccggact gagctggatc aaatgcccgc
     3721 atgcgcagtc aaacgactcc cagccctgac ccttgcgaac gtacgtcatc gaccggctcg
     3781 ctgcgtccgc actggcggag gctgcggccc gcggcgtcgc gtgctcgcgc ccgcagcgcg
     3841 ggcatgccac ttgctcccgc ttgaaattcc caggaatctt caaccgcgca ccgcacgcac
     3901 actggatcac cgccagcggc aacaccgtgt ccagcaggcg tcccacggcc tcagctcgct
     3961 cgaccgcgga tgcacgcggc tccggctccg cagtcggcgc ccgcaatgat gctccctcat
     4021 ctttcgccag cgtgcccgcg ccgatgcatg ccccgccgcg aatctttcgg tacgcctcct
     4081 cataggcggc atagcccgcg ttgcccatgc ggcgcagaat ggcaatgcgc tcactactct
     4141 taggatgcgt cgagaagagg ctgaacgcgg catgttcctg cagcggattg acgatgtaca
     4201 gcggcgccag gacccggttc ttttcagcct ggccgccggc ctgacgcgaa atcttctcca
     4261 acgccgaggc cagtccctcc ggataccgcg tgaagcgcgc gctggacgcg tccgccagaa
     4321 actcgcgccg ccgcgaacac gccaggtaca gcaactgcgc cgccagcggc gccaacaccg
     4381 ccaccagcgc ggctgccaga agaataatca attgcgcctg cccgccaccg cgcgacgaac
     4441 gccgcccgcc gctcccgtaa aacatcccgc gcaggaaacc gcgcgacagc aggacaaccg
     4501 cgcccagcat gacggccgcc agcgtcatga aacgcgcatc caggttgtga atgtgtccaa
     4561 tctcgtgccc gattacgccc tgcagctcgt cccggttgag ccgcttcagc aagcccgccg
     4621 tcaccgccac cgcggccttc tccgggcgcc gcccaacggc aaaggcgttc agcgacgaat
     4681 cgtcgatgat gtacacgcgc ggcatggccg gcaatccggc ggcgatgctc atctcctcga
     4741 ccacattcca cagttgcggc gcctgctcct tcgtaatctc gcgcgccccc gcggacttga
     4801 ggatgatgct atctcccgcc ccgactgcga tcgcccagag aaccagccag atcaccactg
     4861 ccaccgccac accgatcgcg gcggcggctt ccatgctgac taccgggctg actaccgggg
     4921 cctcaggaac gtaatagtag cttggatgag cgcgtgacca gccgccgtcc agttgcgcgg
     4981 ccaccgacat tccgatcacc gccccgagca ggaccagcac accccccatc gtccctatca
     5041 gcacccagga gcggcgtcga ttactggcga ttgcttccca catctgcggg gcgcgtcctc
     5101 aagnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcgcg tgttgtactg
     5221 catgacgctg tcgttgtaga actgccgcgc aaagctgatc ttgttctcag tggaagtcag
     5281 ttcttcctgg agcgccagga agttctggtt tgccttgaga tccgggtagg cctcgaccac
     5341 caccatcaag cgccccaacg cctgcgacag ctctccctcg ctgcgaatgc gatcgtcgac
     5401 ggtctgtgcc ccggtcgccc tggcccgcgc ctggacgact gcgtccagcg tgccgcgctc
     5461 gtgcttcgcg tagcccttga ccgtctcgac caggttggga atcagatcgt gccgccgctt
     5521 gagctgcacg tcgatttgcg cccaggcatt cttgacctgg ttgcgcagcc gcaccagccc
     5581 gttgtacgtg cctatcaccc acagaaacgc caacaacaga acggccacta caaccaacag
     5641 cacaatcatc acaggtccca tcagcttttc tccgtctggc tagtgttctg tcgaaacaac
     5701 tgcccgagaa attccgccac cggcgcgttc ggctcgccgc cgccgacaac cgtcgccaat
     5761 tcgccggcgt caaaccacaa cccatgcccg gccggacagc ggtccacttc aaccggcggc
     5821 cgcgtcccaa ccggctgcac acgcaatctg cgccggcagc gcggacagcg gcgtttatcc
     5881 cggctggttg ggccggggtc gcgcaccgcc gcctcgagcg ggtcggcaac cggcgcctcc
     5941 agcgggttgg tgcgtccgcc ggccagttcc gaaatcagct cgagttcccc ggtgtccagc
     6001 cacacgccgt ggcaatcggc gcagtaatcg acctccaccc cgtcaaattc gaccacgatc
     6061 ataggcttat tgcaggccgg acacacacca cgcctccggg gttaatcacc atctctaagc
     6121 gtagggcatc accatctcta agcgcaggcc atcacaatct ctaagcgcag gccaacacac
     6181 gatgtaagcg tacacgccaa ccggcagtcc gtctcgattg cgagcccgat cgcgccccta
     6241 taattcgcga ctcattttgt aaccgggtgc aatcggtctg ccctcgagtg tcatgtgtaa
     6301 cctcgggtgc aaatgcccaa gctgtaagcg cgggcatgcc cctgtttacc gacatgcggc
     6361 cgcggcccga ctgcgcgaat gggacattcc tggcggcatc cggggcggca cgccccggct
     6421 ctgctcctct tgatcccttg atccctagat cccttgatcc ctcgcccccc tcgggccgcc
     6481 ccggctctgc tctgttattc cgcctcaccc gttgcagctc cggcatcgag ttcatccagc
     6541 agctccaccg ctcgacgcag gtgcgggatc acgatcgacc caccgacgat cagcccgacg
     6601 ttcagcagtt caaagagctg ttcgttcgtc acgcccagct ccttgcagcg gattacgtga
     6661 taggcgatgc aatcgtcgca gcgcagcacc agcgaggcgg ccaagccggc taactctttc
     6721 gttttgccgt caagggcccc atcggcgtac gctttcccgt ccaaggcgaa gaaacggttt
     6781 atgcccagat tgcccgccgc caggatgcgc tcattcatct tctcgcggta cgtgcggaac
     6841 tgttccaatc gcccggccat gcccttctcc cttcagcggt tttcgcgcgg tgctacgtta
     6901 acttgccacg tcaactcgga ccagtgaccc ctgattcgcg gccgtcctaa ctacgtttgc
     6961 caagcacatc aaggcatatc cagaattccg cctccgaggc tccgggcgcg gacctggcgt
     7021 gagcgccggc caggtacgcc taccgcctcg gcacggacgt ggccgacgag gcctccgagc
     7081 tgtgcgtgga gctatcgcgc cagcaccccg gagcggtgat cttcgccggc acgctggtgt
     7141 tcgagcaccc gcactggtac caccgcgtcc tacacaacga gaccgcctac gccatcccgc
     7201 ggcggctcaa atttgccggc attcccgttg tgattctgcc gttattgctg cgccgaccga
     7261 aacacgccgg ccagccaccc gccacgctgc cgagttctcc gtgacgttcg cgcagttttc
     7321 cgcccaccag caccggaaaa tgggcgcaaa aaaaagaggg caccaggggc cctaaggcgt
     7381 cccggtgctc tccggagggg aaagaagcca atttttgagg accgttgctt tctactttct
     7441 ggacccgttt tttaggtccg gtattatcag tcctcgtatt agtataaccg tcgaaaacac
     7501 ctagtcaaga cgaatccaaa attcttttat cgcgatcctg tcatttcatc ggcacgcctc
     7561 aacgcttcag attaacaatt cagcgcgccg ctaacagcat ccgcggatta cggccgtttc
     7621 gctcgtatca accgtaacca caatatgtta cgcacctcaa ccgacttggt tcgtaaaaat
     7681 tatgtgacgt tgtgcttact tttttctaat ccgctttctg gtgaataatt cgccaccccg
     7741 cccgctcatt ttccgatgca aaactcgcta aacagccgcc ccagcaggtt atccaccaac
     7801 tcggcatgag gcctcccttc caagccctcg taagcactgc ggacctccag cgagatcagc
     7861 tcgagctgtt ctggtacgcc cgcgccttca cacaagcctc gcgcccgttc cagcgccgcc
     7921 gttgcggccg ccaatgtgcc gtcgcacacg gccggcggca tcgccacccg gcacacgcac
     7981 tcggcaattt gtcggcgcaa ctcggccatc ccctcgcccc gggccgcact aaccgctacg
     8041 tgccgcccca acacactcgc cggcaccggc gcaggccggt cgccgcccgc cagatccgac
     8101 ttgctcaaaa ccactaagcg acggtcgggc ggaaccacat cgagcacggc cgccatgcca
     8161 gcgtcccacg gaacatccca agcctggacc cacacgagca cgtccgcctg cgccgcgttt
     8221 cgctccgccg ccagatgcgc ggccgccgtc agatcgtccg ccgtccggcc caagccggcg
     8281 cagtcctgca aaacgagttg cacgccgtcg aggcaaattt cggccgacag cacatcgcgc
     8341 gtcgtaccca gcaccggcga gacgatcgcc cgctcgtagc cgagcaatgc nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctcgctgt cccggtcggc gctaatggtt
     8521 gccagaacat cctcgacctg ctcgacgctc agctttcccg ccaggtaggc gcgcgcggtg
     8581 aattcgcccg gcagcgctcg ccgggcgccg cgctcgatca gccgggcgca cagctcacgc
     8641 agcagcggca agcagccggg cacatgcagt tcgaccaggt cctggcccgt gtagctgcgc
     8701 ggagctttga accagcacgc cgtaccggga aactccaatt ggcccgcggt tatgcgcccg
     8761 ttttcggtga tgaacggccc cctgccccca tcggcaccag ccatcggcag cctcaaaccg
     8821 accgaggccg cgtactccca cgcccccggc ccgctgagcc gcacaatacc cagcggactg
     8881 gcctgccacc cgcttgagac cgcgatgatg gtgtcattga tcgcgggaaa gagcatggtg
     8941 cgcgactacg ggctcggctg ggtcgacgga gccggccgcg gcgcgaagtc cacccgcgag
     9001 acccgccagt tgccgtcgtc gccgcgcaaa cattcaaacc gaatcgtcgc ctcgccccgc
     9061 gacccggccg cggttgcatg aacgtccacc tgaaaagccc cagccgcggc gtcgacagcg
     9121 ttcacccggt tcgggtccaa tccatcggcg taatagccga cgaccgctgc ccagcattcc
     9181 acaaaattgt cgacggcggc ttccgggtcc ttcgccgaat gggggtagct ggtctgaaac
     9241 ctgcggcgga tggtgtcgcg tgccgccacc aggtcaagtt gttcgagata ccgactgaca
     9301 gcctcctcgt cacggctggc ccgcgcctcg tgaattgcct tcaagcacag cagcgcgctg
     9361 cgggccgctt gttcggggcc cgacagcaca atcgggatcg ccaccgcttc ctgccccgtc
     9421 gctgccgttg aatgctgcgc cccactgggc cggcaggcca gccagaccat cgccgccagc
     9481 ggcaggaggc ccgcgcctac cagcatcagc ccccgctgca ccgaacgccg gtcccgattc
     9541 gcgtgtgttc gttctctctt tgacatgcgc gagattttaa cccttccaca ccggattgtc
     9601 ggtcacatgc tcatcgcatc cggttcgagt ttctgaaaca ggatcgccgg ctggcccagc
     9661 acgtgtccct cgggcaatgg cacgggcggc tcccaccgaa tttgctcgct gggagcgttc
     9721 agcatggtca gcagccgttt cgcggccgcc ggcatgaacg ggtaagccat ggtcgccaga
     9781 tactgcaccg cgtggatgca cgtcgcgatc gccgccgcac agtcggccgg atcgtccttg
     9841 cgcgtgcgcc acggctcgcg gatgctgaag aactcgttgc acagccgggc gtagcccatc
     9901 atctcttcca acgccgcccg gaagtggtgc ccctcaatct gctggcccac cttggttagc
     9961 gccgcctcac cggccgccag gtgctcgcga tcgcgcggcg tttgggccgt tgccgccggc
    10021 atcttgccat caaagtagcg ggccgggaaa gtcagcgtgc gattcacgaa gttgccgaac
    10081 gccgccacca gttcgctgtt gttccgctgc agaaaatcgg ccgggtcgaa cgaggtacgg
    10141 gccgtttctg gagcgatcgc cgtcaggtaa tagcgcaggg cgtccggatc gaagcgcttc
    10201 agatattcct ggatccaaac aggcgagttc tcggccgtct tgctcttgct gaccttctcg
    10261 tcgccttcgc ccatccggat gttgacgaaa ttgttcgcca ctaccgccga cggaagctgg
    10321 tgccgcccgt cggccatcag catcgccggc cagatcagcg cgtggaagac ggtgttgtcc
    10381 tcgccgatga agtgcacgat cgcggattcc ggatcgcacc accacttctc gtactcgcgc
    10441 cagttcccga gctgatccgc aaagagctgg gccgtgaagg agatataccc gatcggcgca
    10501 tcgaaccaga cgaagagcac tttgccgcgc gcgtccgggt catccagcgg caccgggatg
    10561 ccccaggaga tgtcgcgcgt catcgatcgt tccggcagcc cctgcttgat ctgccccaac
    10621 gcgaagttga gcacgttcgc ccgccactgc ccacgcttgc tctcgagcca ctccttgagc
    10681 ggctgctcga atttttgcag gctcaggaac cagtgcgtcg tctcccgcac ctccgcccgc
    10741 tcgcccgtaa tctcgctgac cgggttgatc agcaggagcg ggtcgatcat ctggccgcag
    10801 ttatcgcact ggtcgccggt cgcgccggcg gcgccgcagt gatggcactt cccgcggacg
    10861 tagcggtcgg gtagaaagcg ggccgctttc ggatcgtaaa gctggcgggt gctcttcttg
    10921 gtgaagtacc ctttttcatg gatgcgcagg aagaactcct ggctgatgcg ctcgtgcatc
    10981 tcgcggtagc cgggctgatg cgtgcccccg taaacgtcga attcgatccc cagcccggcg
    11041 aagtcac
//
LOCUS       DAOSYS010000114        11039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5054_length_11039_cov_5.845776, whole genome shotgun sequence.
ACCESSION   DAOSYS010000114 DAOSYS010000000
VERSION     DAOSYS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5054_length_11039_cov_5.845776"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLU99_08505"
     CDS             <1..918
                     /locus_tag="PLU99_08505"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOO17137.1"
                     /translation="AGYNCSRTLYDAGQGVWLMADALPPTSRPTIYLVSDGTCRTCEN
                     IARAVLVQFGDIDVQIIKRAYVRRPETVRALIRKAAKERAVVFYTLVYDRARAAMQDA
                     AREHLVPVVDLLGPVLSSLYDLFRSAPRAKPGILYTSNKAHFDRVDAVEYTLAHDDGC
                     RPQDLAEADVVLVGVSRTAKSTTCFYLAYSGIRAANVPLFADCEPPPELLALDPRRVI
                     GLTMNPSRLQSVREARLRGWKMDLSDVYARSEHIARELRAANEQMARHGWRCVDTSYK
                     GIEEIAREVMQLLSEPETPTTEHERPVAE"
     gene            1001..3070
                     /locus_tag="PLU99_08510"
     CDS             1001..3070
                     /locus_tag="PLU99_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOO17138.1"
                     /translation="MTSKLDDKKAAPDGTGLIALDPWLEPYAERLRQRYRHYQWMRER
                     AIKAGGSLGHFAERHKYLGFNRGQRDGQPGVWYREWAPAAQALFLAGDFNDWNRKSHA
                     AERDEFGIWHLFLPDAEHGGRFAHGTRVKVFVHSALGPRDRIPAYVRRAVYDPHSHDY
                     CGQYWDPAEPYVWRHAVPELKGNLRIYEAHVGMATPEERIGTYREFADRILPRIVASG
                     YNAVQLMAVQEHPYYGSFGYQVSNFYAPSSRFGTPEDLKYLIDTAHGMGLRVLLDLVH
                     SHAVRNVNEGLNMFDGTDHQYTHAGGRGHHPAWDSLVFDYGKTEVLAFLLSNVRYWLE
                     EFRFDGFRFDGVTSMMYVDHGLGPPFSCYDEYFPLRADPDAVAYLQLANELAHSLNPQ
                     AVTIAEDVSGMVGLARPLDEGGLGFDYRLAMGIPDYWIKLLKDQHDEQWEMGGLFHTL
                     TNRRRGEKHVAYAESHDQALVGDKTIAFRLMDSDMYWHMNKGSQNLVIERGIALHKLI
                     RLITFSLGGEAYLNFMGNEFGHPEWIDFPREGNNWSFKYARRQWNLVDDPLLRYRDLA
                     EFDRAMLELDTKYDLLALPDIELIQVHEDRKLLIYRRGRLVFAFNFHPNGSYPDLRFG
                     VPEAEDYKLVLNSDELWFGGHAEVISDQLYPWQRVAWDDRPQSVQIYLPCRTALVLAP
                     VSLIQEA"
     gene            3070..4650
                     /locus_tag="PLU99_08515"
     CDS             3070..4650
                     /locus_tag="PLU99_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17139.1"
                     /translation="MTNFPGAERKGAGAMCSLRNPPARRRSALPLKVAAALSLAALGG
                     LSMFGCASPVRRHMPVAQHAEGDRKVLAFDMQRAGRPDYWEYVEGDGRVHAIAFAEAS
                     GQPGSPLVLDEMDQSSIPHFVILLDGVPFHMVEEARRDGLFRLFYAPSKVICCYPSMT
                     DLALSDVMHAGACYSLQALGFDRTRNCKCGGSSAYLSSDNAPWVAKVNYRASPWWDGL
                     MYLDPQKVFNHELQGLKKTFAKFQEGEAYGYAVGTAGLGTRYGREGVSKYLCEIDAFC
                     RQLVYERCGRVRITLAADHGHDLTHAQLVSFRDVLEAGGYRETESLRERRDVVVIAFG
                     LVTYAEFYTKDAAGVAQCLLNHEGVEFTCYAAGDKVVIQDCEGFACISQGPGGELRYD
                     SSRGDPLKLNPILERLAHAGKIAPSGEIDAEAMFYATVEHEYPDPVARVWRAFHGLVQ
                     SPPDVIASLRSGYCHGSRFFYEAIGRDVASTHGGLRRDTSTTFVMSMLGDLPPVLRSQ
                     DILPALARLREVRQRSAE"
     gene            complement(4679..5275)
                     /locus_tag="PLU99_08520"
     CDS             complement(4679..5275)
                     /locus_tag="PLU99_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO17140.1"
                     /translation="MTETLRPMEKRDAGAVARLHAETITNGFLRKLGRRFLRQLYLGV
                     AADPGSWVCVAERNGTVLGFLAYSQDVSAMYRRVLRARWWRLGLASLPRSLNLLLIKE
                     ILDTLRYPAKQAAQALPPAEILSVAVDSKARGSGLGRRLVERAVAHAKEDGQPQLKVL
                     AGARLEAANRFYAACGFQKAAELVQHGEVLNVYIKSTA"
     gene            complement(5302..6525)
                     /locus_tag="PLU99_08525"
     CDS             complement(5302..6525)
                     /locus_tag="PLU99_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17141.1"
                     /translation="MHVIYIHQHFGTPQAPDATRSYEMSRALLRAGHRVSMICGLSER
                     NANVLNHDQRVSETLVDGIRVYCIAAQYASRMNFYQRLWSFANFALTAGKVAGGLDGD
                     LVFATSTPLTVGIPGMWAARKLRVPFVFEVRDLWPELAVAVGVLKNPVLIWCARQLEL
                     KTYRSADRIIALSPGMREGIIKAGYPAERVTMIPNGCDLDLFRPSNEPLNDQRFGAPD
                     DFRLVFTGAHGIANGLHAVLDAAAELKRRGVRGVRFVFIGHGQLKPQLMARSRAEGLD
                     ELVSWVDPIPKSELAQILPRLDVGMMILKNVPAFYYGTSPNKFFDYLACGLPVLNNYP
                     GWLADMIGQHKIGRAVPPDDPRAFAEAVIWLREHPEERAAMGQRARALGEAEFSRARL
                     SGDFVKALEGVAAGR"
     gene            complement(6516..7769)
                     /locus_tag="PLU99_08530"
     CDS             complement(6516..7769)
                     /locus_tag="PLU99_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17142.1"
                     /translation="MHVLYLHQYFVTRHGFTGTRSYEFARRLVARGHRVTMLKSGRFA
                     VDGLDTPPGQQYTEVDVEGIRVVPIAAGFANSHKGTGMSGYRRVREFQSFARLATAVG
                     KKLDRPDVVYATSTPLMIGYAGRDLARHFGVPFVFEVRDVWPQALINTGTLRNPLVIW
                     WLRRMERRIYAAADHIVALSPGMKAGVMAAGVPDAKVTVITNGSDADLFRPDLDGSAA
                     RARLGLGQRFAAIYFGAMGRANGLDYVIDAARILHERGREDIVLVLHGSGGMKEALQA
                     RARQLALTNVVFSDSVPDKAAVAELVAGCDACLTIYAATDIEQSWSPNKMFDALAAGK
                     PVLINVPGWLRETVEQNGCGRYTDPKQPEALADALEHLAADRQTCAAQGRQARALFER
                     EFSREILTQRLEAVLNRVTRSMPCT"
     gene            complement(7793..9106)
                     /locus_tag="PLU99_08535"
     CDS             complement(7793..9106)
                     /locus_tag="PLU99_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOO17143.1"
                     /translation="MNITIFGMGYVGIVSAACLLRDGHTVTGVDPNEAKVRDLNAGRT
                     PIQEPGVAELLAAGHASGRLTATAVTPPGLGNCDMAWICVGTPSRPDGGVNTQQVERC
                     IREIGTALREARTRPLLVARSTVPPGTVRNCLIPALEQTSGLRVGRDLHVVFHPEFLR
                     EGCAVDDFDHPPKIVVGEAHPGAADPLLKLYERYTAPRFRVGLEVAEMVKYSDNLFHA
                     VKVVFANEIGALARAVGADARQVAEIFCSDTKLNINPYYLRPGFAFGGSCLPKDLRAM
                     LRLAQQQSIRVPLFEATAESNRVRIEELVERVLSYRPARVGMVGLAFKPRTDDMRESP
                     YVAVAKRLIGEGIELRIYDPGVDTRRLLGSNQAAVQTALGHLERLLVASLDEFAQCEL
                     ILVNHPTVTAECVTGWLKSGLRVIDLVGIPGLGPPASPGYEGIAW"
     gene            complement(9103..>11039)
                     /locus_tag="PLU99_08540"
     CDS             complement(9103..>11039)
                     /locus_tag="PLU99_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOO17144.1"
                     /translation="GRQPNLAERIRLLQQGRCVYFFKLVPPQPVEWNRNPFDGSHSDA
                     TKTWTDLPEYAPQQGDYRMLWEPARAAWAIDLTRAPAHGLDCDAAELYWQWVDSWMAA
                     CPPFRGVQWKCGQEAAVRLLALTFGFWSLAASPVTTPARWRQFARLAWATAYRIAHYI
                     DYAISQKNNHAISEACGLMLVGHLFPEFAESKRWWRRGKRVLAGELRRQIYSDGSYVQ
                     HSMNYQRVMLAMAAVAFRLAELHGDPFERGLYDKLSLANDFLFEMMDPQSGGVPNYGN
                     NDGAHVLPLDECDFVDFRPIVQTVHYLVHRQRRLEPGPWDEDLLWLFGPEALAAPRSP
                     REPRAHAFADGGYFTLRGDQSWALVRCHTYRDRPAHYDPLHLDLWYKGHNVLRDCGTF
                     KYYIPGRPDLERYFRSIQAHNCVEIDGGMPVELASRFQFFPWPRCRARQYNPVPASSG
                     SCSCIFENHDYDRAPWHVLHRRTLLQLPGDAWVVVDDLLGIGRHRAVWYWHLLDADLT
                     LEPGQTALVLKTPAGAYALAASATVAPRRFEIVRGRDELGRVQGFAAPYYGERRPIPV
                     LEVEYEAHLPLRVVTALGPIRAMATRLADVDGQERWVVSAGDASFELTLPPPTRKIAQ
                     LLLECPVALAPSESRKDENR"
BASE COUNT         1909 a         3407 c         3723 g         2000 t
ORIGIN      
        1 gccggttaca actgctcccg cacgctgtac gatgccggcc agggagtttg gctgatggca
       61 gatgcgttgc cgccgacctc gcggccgaca atctacctcg tttcagacgg cacctgccgc
      121 acctgcgaga acatcgcgcg ggccgtgctg gtgcagttcg gcgacatcga cgtccagatc
      181 atcaaaagag cttacgtccg gcggccggaa accgtacgcg cgctgatccg caaggcggcc
      241 aaggagcggg cagtcgtctt ctacacgctg gtttacgacc gggcgcgggc cgcgatgcag
      301 gatgcggccc gcgagcacct ggtgccggtg gtggacctgc tcggccctgt tctaagcagc
      361 ctgtatgacc tcttcaggag cgcgccgcgg gccaagccgg gaatcttgta tacgtcgaac
      421 aaagctcatt tcgatcgcgt cgatgccgtc gaatacacgc tcgcgcacga tgatggctgc
      481 cggccgcagg acctggccga ggcggacgtc gtgctggtag gcgtctcgcg gacggccaag
      541 agcaccacgt gcttttacct ggcctacagc ggcatcaggg cggccaacgt gccgctgttc
      601 gcggattgcg agccgccgcc ggagttactc gcgctggacc cgcggcgggt aattgggctg
      661 accatgaacc cctcgcgcct gcaaagcgtg cgcgaggccc ggctgcgcgg ctggaagatg
      721 gacctgagcg acgtttacgc ccgcagcgag cacattgcgc gcgaattgcg ggccgcgaat
      781 gagcagatgg cgcgtcacgg ctggcgctgc gtggatacat cgtacaaggg catcgaagag
      841 atcgcccgcg aagtcatgca gctcttgagc gagcccgaaa cgcccaccac ggagcacgag
      901 cggccggttg cggaatagca ggcgggcggc tggccggttt tagacggcat acgcttgcac
      961 cgctatcctc attacaaaag caggtgaggg cgagaacaac gtgaccagta aattggacga
     1021 caagaaggca gcacctgacg gcaccggcct aatcgcgtta gatccgtggc tggaaccata
     1081 cgcggaacgg ctgcggcaac gttaccggca ttatcagtgg atgcgggaac gtgcgattaa
     1141 ggccggcggc tcgctggggc acttcgccga gcgtcacaaa tatctgggct ttaaccgcgg
     1201 gcagcgtgac gggcaaccgg gcgtctggta tcgcgaatgg gcgccggccg cccaggcctt
     1261 gttcctggcg ggggacttca atgactggaa ccgtaaatcg cacgcggcgg agcgcgacga
     1321 gtttggaatc tggcatctct ttttgccgga tgccgagcac ggcggccggt tcgcgcacgg
     1381 cacgcgcgtg aaagttttcg tccacagtgc gctggggccg cgcgaccgca tcccggccta
     1441 cgttcgccgg gccgtttatg acccgcattc tcacgattac tgcgggcagt actgggatcc
     1501 tgcggagcct tatgtatggc ggcacgctgt gccggagctg aagggtaacc tgcgcattta
     1561 cgaggcgcac gtcggcatgg cgacgccgga ggagcggatc ggtacgtacc gcgagttcgc
     1621 cgaccgcatc ctgccgcgca tcgtggccag cggttacaac gccgtgcagt tgatggccgt
     1681 gcaggagcat ccgtattacg gctcattcgg ctaccaggtc agcaatttct acgcgccttc
     1741 ttcgcgattt ggcacgccgg aggatttgaa gtacctgatc gacacggccc acggcatggg
     1801 gctgcgtgtt ttgctggacc tcgtgcacag ccacgccgtc cggaacgtca acgaaggttt
     1861 gaacatgttc gacgggacgg accatcagta cacgcacgcc ggcggccgcg ggcaccatcc
     1921 ggcctgggac tcgctcgttt tcgactacgg caaaactgag gtgctggctt ttctgctgtc
     1981 gaacgtgcgg tattggctgg aggaattccg cttcgacggt tttcgcttcg acggcgtcac
     2041 aagcatgatg tacgtcgatc acggcctggg cccgccgttc agttgttacg acgagtattt
     2101 cccgctgcgg gccgacccgg acgcggtggc ctacctgcag cttgcgaacg agctggcgca
     2161 ttcgctgaac ccgcaagcgg tgacgattgc cgaggacgtc tcgggcatgg tggggctggc
     2221 ccggccgttg gacgaaggcg gcctgggctt tgattaccgg ctcgccatgg gcatcccgga
     2281 ttattggatc aagctcctga aggatcagca tgatgagcag tgggagatgg gcgggctgtt
     2341 ccacacgctg accaatcggc ggcggggcga aaagcacgtg gcgtacgccg agagccatga
     2401 ccaggcgctg gtgggcgaca agacgatcgc ctttcgcctg atggactcgg acatgtactg
     2461 gcacatgaac aagggcagcc agaacctggt catcgaacgc ggcatcgcct tacacaagct
     2521 gatccggctg attaccttct cgctgggcgg cgaggcgtac ctgaacttca tgggcaacga
     2581 gtttgggcat ccggagtgga tcgacttccc gcgcgagggg aataattggt cgtttaagta
     2641 tgcccgccgg cagtggaacc tggtggacga cccgttgctg cgctaccgcg acctggccga
     2701 gtttgaccgg gccatgctcg agctcgacac caagtatgat ctgctggcgc ttcctgatat
     2761 cgagctgatc caggtccacg aggaccgcaa gctgctgatc taccggcgcg ggcggctggt
     2821 gttcgcgttt aacttccacc ccaacggttc ctaccccgat ttgcggttcg gcgttccgga
     2881 ggccgaggac tacaagctgg tcctcaacag cgacgagctg tggtttggcg ggcatgccga
     2941 agtaatctcc gaccagttgt acccgtggca gcgggtggcc tgggacgacc gtccgcaaag
     3001 cgtgcaaatt tatctgccct gccggactgc ccttgtgctt gcgccggtgt ctctaattca
     3061 agaggcttga tgacgaactt ccctggtgct gagcgtaagg gggccggagc gatgtgctcg
     3121 ttgcgtaacc cgccggctcg ccgcagaagc gcgctgccgc tcaaagttgc ggccgcgctg
     3181 agcctggctg ctctgggcgg gctgtcgatg tttggctgcg cctctccggt gcggcgtcat
     3241 atgcctgtgg cgcagcacgc cgagggcgat cggaaggtgc tggcgttcga catgcagcgc
     3301 gcgggccggc cggattactg ggaatacgtt gagggggatg ggcgcgtcca cgcgatcgcg
     3361 tttgcggagg cgtctggcca gccaggttcg ccgctcgtgc tggacgagat ggaccaatcc
     3421 agcattccgc acttcgtgat tctgctggac ggcgtgccgt tccacatggt ggaggaggcg
     3481 cggcgcgacg ggctgtttcg gcttttttac gctccgtcaa aggtgatctg ttgttatccc
     3541 agcatgacgg atttggcgct ctcggacgtg atgcacgccg gagcgtgcta ctcgctgcag
     3601 gcgctgggct ttgaccggac tcgaaactgc aagtgcggcg gcagcagcgc ttatttaagc
     3661 tcggacaacg cgccctgggt ggcgaaggtg aattaccgcg cttcgccctg gtgggacggc
     3721 ttgatgtacc tggacccgca gaaggttttc aaccacgagc tgcagggttt gaagaagacg
     3781 ttcgcgaaat tccaggaggg cgaagcttac ggctatgcgg ttggcacggc gggcctgggc
     3841 acgcgctacg ggcgggaagg cgtgtccaag tacctttgcg agatagacgc cttttgccgg
     3901 cagctcgttt atgagcgctg tggccgcgtg cgaattacgc tggcggccga ccacgggcac
     3961 gatctgaccc acgcccaact ggtctcgttc cgcgatgtgc tggaggccgg tggataccgt
     4021 gagactgaat cgcttcgcga gcggcgcgac gtggtagtta tcgccttcgg gctggtcacg
     4081 tacgcggagt tctacacgaa ggatgccgcg ggcgtggccc agtgcctgtt gaatcacgag
     4141 ggcgttgaat tcacgtgcta cgcggccggc gacaaggtcg tcattcagga ttgcgaaggg
     4201 ttcgcctgca tttcacaagg ccccggcggc gagttgcgat acgacagcag ccgaggtgat
     4261 ccgctgaagc tgaaccccat tctggagcgc ctcgcacacg cgggtaagat cgcgccgagc
     4321 ggggagatcg acgcggaggc catgttctac gcgacggtcg agcatgagta ccccgacccg
     4381 gtagcgcgcg tgtggcgggc gttccatggc ttggttcaga gcccgcccga tgtcattgcc
     4441 agcctgcgca gcggatattg tcacgggtcg cggttctttt atgaggcgat cggccgggac
     4501 gtcgccagca cgcatggcgg tctgaggcgt gacacctcca cgaccttcgt gatgagcatg
     4561 ctcggcgacc tgccgccggt gctgcgctcg caagacattc tgccggcgct ggcgaggttg
     4621 cgcgaggttc ggcaacgctc ggccgaatga acgattccat tgctggttgc tatttgccct
     4681 acgccgtgga tttgatgtag acgttcagca cttcgccgtg ctgaacgagt tcagccgctt
     4741 tttggaaacc gcaggcggcg tagaaacggt ttgcggcctc gagccgcgcg cccgctaaca
     4801 ccttgagttg cggctggccg tcctcttttg cgtgtgccac ggcgcgctcc accaggcggc
     4861 ggccaagccc tgagccgcgg gccttggaat cgactgccac actgaggatt tcggccgggg
     4921 gcaaggcctg cgcggcctgc ttcgccgggt agcgcaaggt gtccaggatt tccttgatca
     4981 acagcagatt aagcgaccgt ggcagcgacg ccagcccaag acgccaccag cgcgcccgca
     5041 gcacgcgtcg gtacatggcg cttacatcct gcgagtaggc cagaaacccg agcaccgtgc
     5101 cgtttcgctc cgcgacgcat acccatgaac cggggtctgc cgccacgccg aggtaaagtt
     5161 gccgtaggaa acgtcggccc agtttgcgaa ggaacccgtt cgtgatggtt tcagcatgca
     5221 ggcgtgccac cgcgcccgcg tcgcgtttct ccattggccg gagcgtctca gtcacactgt
     5281 gcacccttgc tcgacgtgct cttagcggcc cgccgcgacc ccttccagcg cctttacgaa
     5341 atcgcctgag agccgcgcgc gggagaattc ggcctcgccc agcgccctag ctcgctgccc
     5401 catggcggcg cgctcttccg ggtgctcgcg cagccatatc acggcttccg cgaaggcccg
     5461 cgggtcgtcc ggcggaacgg cgcgcccgat tttgtgctga ccaatcatgt ccgccaacca
     5521 gccgggatag ttgttcagca ccggcagtcc gcacgcgagg tagtcgaaaa acttgttcgg
     5581 cgacgtgccg tagtaaaacg ccggcacatt cttcaagatc atcatgccca cgtccaggcg
     5641 cggcagaatc tgagccagct cggacttcgg gatcgggtcc acccaactga ccaactcatc
     5701 cagtccttcc gcgcggctgc gagccatcag ttgcggtttg agctgtccat gcccaatgaa
     5761 aacgaaacgc acaccgcgca cgccccgccg tttcagctcc gccgccgcgt ccagtaccgc
     5821 gtgcaaaccg ttggcgatgc cgtgggcgcc cgtaaacacc aggcggaaat cgtccggtgc
     5881 tccaaaccgc tggtcattta gcggctcatt gctgggccgg aacaaatcga gatcgcagcc
     5941 gttcgggatc atggtcaccc gctcagcggg gtatcccgct ttgatgatgc cttcgcgcat
     6001 cccgggcgat aaagcgatga ttcggtctgc ggaacggtag gtcttcagtt caagctgccg
     6061 ggcgcaccag atcagtaccg ggttcttcaa cacccctacg gcgaccgcga gttccggcca
     6121 caagtcgcgc acctcgaaca cgaacgggac gcgcaatttg cgggctgccc acatgcccgg
     6181 aattccgacc gtcagagggg tcgaggtggc gaagaccaga tcgccgtcca gcccgcccgc
     6241 cactttgccg gcagtaaggg cgaagttggc gaacgaccac agccgctggt agaagttcat
     6301 ccggctcgca tactgtgcag caatgcagta cacccgtatt ccatcgacga gcgtctcact
     6361 gacgcgctgg tcgtggttga gcacgttcgc attgcgttca ctcagcccgc agatcatgct
     6421 gacgcgatgc ccggcgcgga gcagggcccg gctcatttcg taggagcgcg tggcgtccgg
     6481 ggcctggggc gtgccgaagt gctggtggat gtagattacg tgcacggcat gctccttgtt
     6541 acccggttga gaaccgcttc aaggcgctgc gtcaggattt cgcgcgaaaa ctcgcgttcg
     6601 aagagcgcgc gggcttggcg gccctgtgcg gcgcaggttt ggcgatcggc cgcgaggtgt
     6661 tccagcgcgt cggccagggc ctcgggctgt ttcgggtccg tgtagcggcc acagccgttt
     6721 tgctcaacgg tctcgcggag ccagccgggg acgttgatga ggaccggttt gccggccgcg
     6781 agggcgtcga acattttatt gggcgaccag gactgctcga tgtcggttgc ggcgtaaatg
     6841 gtcaggcagg cgtcgcagcc cgcgaccagt tcggcgacgg cggccttgtc ggggacagag
     6901 tcgctgaaga cgacgtttgt cagggcaagt tgccgagcgc gggcctgcaa cgcctctttc
     6961 atgccgccgc tgccgtgcaa cacgaggacg atgtcctcgc ggccgcgctc gtgcaggata
     7021 cgggcggcgt cgatgacata gtccagaccg ttggcccgcc ccatggcccc gaagtagatc
     7081 gcggcgaagc gctgccccag cccgagccgc gcgcgtgcgg ccgagccgtc caggtcgggg
     7141 cggaacaggt cggcgtccga gccgttggtg atcacggtca ccttggcgtc cggcacgccg
     7201 gcggccatta cgcccgcctt cattccggga gagagggcga cgatgtggtc ggccgcggcg
     7261 taaatgcggc gttccatgcg tcgcagccac caaatcacga gcgggttgcg cagagtgccg
     7321 gtgttgatga gcgcctgcgg ccagacgtcg cgaacttcga agacgaacgg cacgccgaag
     7381 tggcgggcga ggtcgcggcc ggcgtagccg atcatgagcg gggtgctggt ggcgtaaacc
     7441 acatcggggc ggtccagctt tttgccaacg gcggtagcca gcctggcgaa gctctggaat
     7501 tcgcgcaccc ggcggtagcc gctcatgccg gtgcctttgt gtgaattcgc gaagccggcc
     7561 gcgatcggta cgacgcgaat gccttcgacg tcgacttcgg tgtattgctg cccgggcggc
     7621 gtgtcgaggc cgtcaaccgc gaaccgtccg gacttgagca tcgtcacgcg gtggccgcgg
     7681 gccacgaggc ggcgggcgaa ctcatacgag cgcgtgccgg taaacccgtg tcgcgtgacg
     7741 aagtactgat gcaggtagag aacgtgcaca agcgctcctc agcgacggct gctcaccagg
     7801 cgatgccctc gtagccgggc gacgccggcg gcccaagacc cggaataccg accaggtcga
     7861 ttacgcgcag gccggacttg agccagcccg tcacgcattc ggctgtgacg gtcgggtggt
     7921 tgaccaagat caattcacac tgggcgaatt catcgaggct ggcgaccaac agtcgttcca
     7981 ggtgcccgag cgctgtctgc acggccgcct ggttgctacc cagcaggcgg cgcgtgtcca
     8041 cgccggggtc atagatgcgc agctcgatgc cttcgccgat caggcgtttg gcgacggcga
     8101 cgtacgggct ttcgcgcatg tcgtcggtgc gcggcttgaa cgcgagaccg accatgccga
     8161 cgcgcgccgg acggtagctg aggacgcgct cgacgagttc ctcaattcgt acgcgattgc
     8221 tctccgcggt ggcttcgaac agcggcacgc ggatcgattg ctgctgcgcc agacgcagca
     8281 tggcgcgcag gtccttcggc aggcaggagc cgccgaaggc gaagcccggg cgcaggtaat
     8341 acgggttgat gttgagtttg gtgtccgagc agaaaatttc ggcgacctgg cgtgcgtcgg
     8401 cgccgaccgc gcgggccagt gcaccaattt cgttagcgaa cacgaccttc acggcgtgga
     8461 acaggttgtc cgagtacttg accatctcgg ccacttccag tccgacgcga aagcgcggcg
     8521 cggtgtagcg ttcatagagt ttcagcaacg gatccgccgc gcccggatgc gcctcgccga
     8581 cgacgatttt gggcggatgg tcgaaatcat ctacggcgca accctcgcgc agaaattcgg
     8641 gatggaagac cacgtgcagg tcgcgtccga cgcgcagccc gctggtctgc tccaacgcgg
     8701 gaatcaggca gttacgaacg gttcccggcg gcacggtgct gcgcgcgacg agcagcggcc
     8761 gcgtgcgcgc ctcgcgcagg gccgtcccga tttcgcgaat gcagcgctcg acctgctgcg
     8821 tgttaacgcc gccgtcgggg cgcgagggcg tgcccacgca aatccacgcc atgtcgcagt
     8881 ttcccaggcc cggcggagtg acggcggtcg ccgtcagtcg tccggaggcg tggccggcgg
     8941 ccagcagttc ggcgacgccg ggctcctgga tgggcgtgcg gcccgcgttg aggtcgcgca
     9001 ccttggcttc gtttggatcg acgccggtaa ccgtgtgtcc atcgcgcagc aggcaggccg
     9061 cgctgacgat tccgacgtaa cccatgccga agatggtgat gttcacctgt tttcgtcctt
     9121 ccgcgattcg cttggcgcga gcgcaaccgg gcattctagc agcagttgcg cgatctttcg
     9181 cgttggcggc ggcaaggtca gttcgaagct ggcgtcgccg gcgctgacga cccagcgctc
     9241 ttgtccgtct acgtcggcca gccgggtcgc cattgctctt atcggtccga gagcggtcac
     9301 gactcttaag ggcaggtggg cttcgtattc aacctccaga accgggatcg gccggcgctc
     9361 gccgtagtac ggggcggcaa agccctgcac gcggccgagc tcgtcgcggc cccggacgat
     9421 ttcgaaccgg cgcggagcga ccgtggcgct ggccgccagg gcataagcgc cggccggtgt
     9481 tttcaatacg agcgccgtct ggccgggttc gagcgtcagg tcggcgtcga gcaggtgcca
     9541 gtaccaaacc gcgcggtgcc gaccaattcc aagcaagtca tctacaacga cccaagcgtc
     9601 gccgggcagt tgcagcagcg tgcggcgatg cagaacatgc cagggcgcgc gatcataatc
     9661 gtggttttcg aagatgcagg agcatgagcc actcgaggcc ggaaccgggt tgtactgacg
     9721 cgcccggcag cgcggccacg ggaagaattg aaaacgcgag gccagctcaa ccggcatgcc
     9781 gccgtcgatc tcgacgcagt tatgggcctg gatcgaccgg aagtaccgtt ccaggtccgg
     9841 gcgcccggga atgtagtact tgaacgtgcc gcagtcgcgc aggacgttgt gccccttgta
     9901 ccacaggtcc aggtgcagcg gatcatagtg tgcggggcgg tcgcggtagg tgtggcaacg
     9961 caccagggcc caactctgat caccgcgcag cgtgaagtat ccgccatcgg caaacgcgtg
    10021 cgcgcgcggc tcgcgcggcg acctgggcgc ggccagcgcc tccggtccga agagccacag
    10081 cagatcttca tcccacggcc cgggctccag ccgacgctgc cggtgaacga ggtaatgcac
    10141 cgtctgcacg atcggccgaa agtcgacgaa gtcgcactcg tcgagcggca ggacgtgggc
    10201 gccgtcatta ttgccgtagt tcggaacgcc gccgctttgc gggtccatca tttcgaagag
    10261 gaaatcattc gcgaggctca gtttatcgta caggccgcgc tcgaacgggt cgccgtgcag
    10321 ttcggccagg cggaaggcca ccgcggccat ggccagcatg acgcgctggt agttcatgga
    10381 atgctggacg tagctgccgt cgctgtagat ttggcggcgc agctcgccgg ccagcactcg
    10441 tttaccgcgg cgccaccagc gcttggactc ggcgaactcc gggaatagat ggcccacgag
    10501 catcaagcca caggcctcgg agatggcgtg gttgttcttt tgcgagatcg cgtaatcgat
    10561 gtagtgggcg atgcggtagg cggttgccca ggccaggcga gcgaactggc gccagcgggc
    10621 cggtgttgtg acgggactgg cggccaggct ccagaacccg aaggttaagg ccagcaggcg
    10681 gactgccgct tcctgtccac atttccactg gacgccgcgg aatggcgggc aagccgccat
    10741 ccaggagtcg acccactgcc agtacagctc cgccgcgtcg caatccaagc catgtgccgg
    10801 cgcccgggtc aggtcgatcg cccaggccgc ccgcgcgggc tcccatagca tgcggtaatc
    10861 gccctgctgg ggtgcgtact cgggcaggtc cgtccaggtt tttgtcgcgt cggagtggct
    10921 gccgtcgaat gggttgcggt tccactcgac cggttgcggc gggaccagct tgaagaagta
    10981 gacgcagcgg ccttgctgca gcaggcggat gcgttccgcg aggtttggtt ggcggccgg
//
LOCUS       DAOSYS010000115        10988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5082_length_10988_cov_5.777097, whole genome shotgun sequence.
ACCESSION   DAOSYS010000115 DAOSYS010000000
VERSION     DAOSYS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5082_length_10988_cov_5.777097"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLU99_08545"
     CDS             complement(<1..370)
                     /locus_tag="PLU99_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17145.1"
                     /translation="MKRSVVVWVAVLATGLVASAAERVVQCEEFTATSCGYCPYAGEA
                     FGALLNSYPTTFAGIQIHLGDAYATSWGTARDTFYNVTGTPTAWFDGLISRVGGTSGM
                     TYTSEYNTRHNIPSDIEMLVG"
     gene            complement(705..1286)
                     /locus_tag="PLU99_08550"
     CDS             complement(705..1286)
                     /locus_tag="PLU99_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17146.1"
                     /translation="MSSGKFLRTDPSRAQAAMLAVGAVANFYWRGPFGLLPFLIFTLA
                     PVAVAVSVLNRLHLARHVLPVAAGLLSLGSSWLIAAPPLTRTLFSRHLGVARPAGIVQ
                     RWDQNWARDRAYLLQFSASPQTIDEIVERSGMRPMSRTPLFSNPFFLSEAPAWWQSGG
                     TSTAGAWEKEAGLLIQLRYDASDGLAQLGFFER"
     gene            1454..3934
                     /locus_tag="PLU99_08555"
     CDS             1454..3934
                     /locus_tag="PLU99_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO17147.1"
                     /translation="MDVTGFLQRLTATRDYRGQIVAVRSSEPRPAAYRTPAAPLPDRL
                     WSLLRQEGIEQLYSHQADAYDAVQAGESVVVASGTASGKSLCYQLPILAALLNDPEAR
                     ALCLYPAKALAYDQLGNLERMVATGEIADVAKPACYDGDTPTHKRAGIRRSASILLSN
                     PDMLHQSILPYHPKWAGFFARLKYVVLDEIHTYRGIFGSHVAGVIRRLQRICRHYGGA
                     PQFICCSATLANPRELAERLTNRPMRLIDQDGSPRGRRWFVLWNPPWLDTPTRITRRS
                     ANIEAQDLLRDLLEEGAGTIAFAKARVVAELLYKYVADSLRRRQPELARKLRPYRGGY
                     LPLERREIERALFSGELLGVCSTNALELGIDVGSLDAALIVGFPGTLCSLWQQAGRAG
                     RRQDESLAIFVAYDDPVDQYLMRNADFIFKRPLEQAIIDPQNPHILAAQLACAARELP
                     LTPADLTTFGTLAEPVANELVQAGRLKAGQDRYDYSVAGLPHHETNLRTISNATYAIV
                     DITGGRNEVIGQVDSISAPELVYPEAIYLHQGDSYIVRALDLSARLARVERMDADYYT
                     QPVLADECRIVAERLTADCLGGRRSFGDVTVRWQTVAFRKFKFRTMELIGQTALDLPA
                     QQVNTTGCWLQPPPAAIEATNAALAAAAASVTPSPGAGIPSSGVAIPSPGARAVPDPG
                     ARAIPSLGISATLSPGATLFDAFSGVRNLLAVALPTLAMCDRYDIGGVVDASQLGVMT
                     IILYDRYEGGVGYARHGYECAADLLKLAYDIVAGCDCDNGCPGCVGPPNLRVAIHHDP
                     DLGRGYKIPDKAATMTLLRAWLDRSASK"
     gene            4070..4744
                     /gene="pgmB"
                     /locus_tag="PLU99_08560"
     CDS             4070..4744
                     /gene="pgmB"
                     /locus_tag="PLU99_08560"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOO17148.1"
                     /translation="MLGRNPGSAVIFDLDGVLTDTAESHFQSWMDVSREFGIPFDRAA
                     NDALRGLSREDSLRLFLGPHASRFSAAEQTQIITRKNDRYLARIERMSTADLAPGARE
                     LLQDLRRLSIPAAVASSSRNAVQVLERLGIRPLLDAVVDGNDVPVSKPDPTVFLCAAE
                     RLGVQPQRCVVVEDAASGVAAGLAAGMRVVGLGPPQRVGRAHLVLDSLAELDARRILA
                     LLPSDD"
     gene            complement(4863..4945)
                     /locus_tag="PLU99_08565"
     tRNA            complement(4863..4945)
                     /locus_tag="PLU99_08565"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4909..4911),aa:Tyr,seq:gta)
     gene            complement(5165..7117)
                     /locus_tag="PLU99_08570"
     CDS             complement(5165..7117)
                     /locus_tag="PLU99_08570"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOO17149.1"
                     /translation="MSSALLWKPSEDRVHQSQMFQFMTTVASRHGVQPDWQSLRRWSI
                     ERRDLFWNDLLEFAEVKPSRPADATMRGEGMLGTKWFPGMTLNYARHMLRFNDDQDAI
                     LAEDERGRAVRLSHRELRDEVARCAAALRQAGVKKGDRVAGFMPNVPETVIAMLAAAS
                     LGAVWSSCSPDFGVRGVLDRFGQIEPSVLVVTDGYTYNGKRCPTLPRVAEMLSQLKGV
                     RRVAVVPFMDEQPSLAGLPNAVLWREWLGPLDRAAPPLTFEEVPFDHPLFIMYSSGTT
                     GIPKCIVHGHGGTLLQHMKELMLHCDLRRGDRSFYFTTCGWMMWNWLVSALGIGTAVV
                     LFEGNPAYPDAKRLWQLAEKLRVTLFGTSPKFLGMCQKAGLRPGREHDLSALRIICST
                     GSPLSEELFRWTYDEVKRDVQLASISGGTDIISCFMLGNPMLPVYAGEIQCTGLAMDV
                     QAWDADGRPVIGEKGELVCTRPFPSQPVCFWNDPDGRKYRSAYFDYYPGRCIWRHGDF
                     IEITERGGVIVYGRSDATLNPGGIRIGTAEIYRVVEGMPEIVDSIVVGRDTPDADVEV
                     CLFVVLREGLKLDEALEKKIRTAIAEGATKRHVPKLIRQVSAIPHTISGKKVEMAVRQ
                     VLHGQDVSNRDALANPEALDEFKGLA"
     gene            7334..8281
                     /locus_tag="PLU99_08575"
     CDS             7334..8281
                     /locus_tag="PLU99_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO17150.1"
                     /translation="MQREFDRRAFLKSAGAAVGAVSLAGALGFAPATGEAPQAQPPSH
                     AGGRRCKLQKSLKYGMVAGGASVLDKFELAKKAGFEGIELDSPNTLDADEVLRARDVT
                     GLVIDGVVDSVHWRQTLGDPDPAVRAAGRQALETALRDCRLYGGTTVLLVPAVVSKKI
                     SYADAYARSQEEIRKVLPLAAELRVTIAIENVWNAFLLSPLEAARYTDELNSPWVGWH
                     FDVGNVVNFGWPEQWIRTLGKRIVKLDIKEYSRKKRDEQGPGAGFAVELGEGDCDWPA
                     VMEALDEIGFQGWAAAEVGGGDLERLKKIATTMDRLFNS"
     gene            complement(8475..>10988)
                     /gene="lon"
                     /locus_tag="PLU99_08580"
     CDS             complement(8475..>10988)
                     /gene="lon"
                     /locus_tag="PLU99_08580"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOO17151.1"
                     /translation="FPGTVVPLSIGREKSRLLTDAVLAGDKLLALFTQRHEEVEDPGL
                     DDLYRIGTAAQVLKVLKLPDGSNSLLVHGLVRVGIEELVATDPYWRAVVNPHIDQVTP
                     SLELEALVHNARQIAREVIELSPNVPDEARLVLDNIDRPDALADFLAANLSLGVVQKQ
                     ELLETFDVVDRLKKVTAILNNQVEVLRLSQKIQDDVRREIDKTQRDFYLHEQLKAIQK
                     ELGETDGTAGQIEEFRKRIEAAGMPDAVRQEAERELKRLERVPQASPESGVIRDYLTW
                     LCELPWNKETPDNLDLDRAEAILNEDHFGLTNVKRRIIEYLAVRKLNPASKGPILCFV
                     GPPGVGKTSLGLSIARALGRSFIRIALGGVRDEADIRGHRRTYIGAIPGRIVQEIRKA
                     GTRNPVLMLDELDKLGADFRGDPAAALLEVLDPAQNHSFTDHYLSVPFDLSKVLFIGT
                     ANYIDPVAPALRDRMEIIDLPGYTLFEKFEIAKRYLVPRQLAENGLADRNVTFSDDAI
                     RAIIERYTREAGVRNLERSIGTVCRGLAAKVARNKVADVAVDPEDLVEYLGPPRFERE
                     LALERGMPGVVTGLAYTPVGGEIIFVEATAMEGRGNFALTGQIGEVMRESGLAALSII
                     RSRARQWGLKLPDLGKTDIHIHVPAGGVPKDGPSAGVAMVCALASLFAGQIADPSVGM
                     TGEITLRGQVLPIGGVKEKVLAAHRAGLKKVVLPARNEPDLQEVPKEVRDELIFAFSR
                     TIEDVLTQVLPERKWPSAPTAGEIGSSSDRVPEPQEAAVGKPGRKASAAKAAPQRATA
                     QKTETKTGLSRDKRAKVRAGSVRRRPNTQAAAQDRGSRN"
BASE COUNT         1919 a         3619 c         3546 g         1904 t
ORIGIN      
        1 cgccgaccag catctcgatg tcgctcggaa tgttgtggcg ggtgttgtac tccgaggtat
       61 aagtcatgcc ggatgtgccg cccacccgcg agatgaggcc gtcaaaccag gccgtcggcg
      121 ttcccgtaac gttgtagaag gtgtcgcggg ctgtccccca ggatgtggcg tacgcgtcgc
      181 cgaggtgaat ctggatgccg gcgaaggtgg tgggataact attgagcagc gctccgaatg
      241 cctcgccggc gtatgggcag taaccgcagc tcgttgccgt aaactcctcg cactgaacta
      301 ctctctcggc ggctgaagcg accagaccgg tcgcgaggac cgctacccat acaacaacag
      361 accttttcat ctctcgttac ctccaaaacg tgaacagaca cgcgcgcccg acgccggccg
      421 cgctgtactc ggcggtagat gcaatctcgt cccgaacgta gcgccggttc aaccgacatg
      481 gtttcgagtg tagccggagg gcagccccct cccaagcggt tttttggaca atctcgccga
      541 aacgggatca ggtcgcaggc aacgaagccg gcggcattag aggacccgcg accgccatcg
      601 acccacagcc gctggccagc gtgctcgtca ggaggcgcgc gacgttctcg ctgtgctgac
      661 aaacgacggg atcctcagtc aagtctcgct tgggttattc gagcctaccg ctcaaagaat
      721 ccaagctgag cgagcccatc agaggcgtca tacctaagtt gaataaggag ccccgcttct
      781 ttctcccagg cccctgcggt gctcgttcct ccagattgcc accatgctgg cgcttcgctc
      841 agaaagaagg gattcgagaa aagcggcgtc cgactcatcg gccgcattcc agagcgctca
      901 acaatttcgt cgatcgtctg cggtgacgcg ctgaactgca gcagataggc gcggtcgcgt
      961 gcccagttct ggtcccatct ctgaactata ccggcgggcc gcgcaactcc caaatggcgg
     1021 ctgaacaggg tccgcgtgag cggcggcgcg gcgatcaacc acgagctccc taaggagagc
     1081 aaaccggccg cgactggcag aacatggcgc gcgagatgca aacggtttag cacgctcacc
     1141 gccaccgcca caggtgcaag cgtaaatatc aggaacggca acagcccgaa cggaccacgc
     1201 cagtagaaat tggcaacagc gccgacggcc agcattgcgg cctgcgcgcg gcttggatca
     1261 gtcctcagaa atttgccaga tgacattgtg cctatccacg catcagtgta actcctgccg
     1321 cacgcgctcc gccagttcgc cgatttgctc cgacgcgccc gcaccgccag ccgcgccgtc
     1381 aaaacccgcc ccgcgaccca tccgagccgc caaccctcta ccccgctata ctgccctcgg
     1441 agtacccgac aacatggacg taaccggctt cctacagcga cttaccgcaa cccgcgacta
     1501 tcgcggccag atcgtcgcgg tgcgctcctc ggagccgcgg ccggctgcct atcgcacccc
     1561 ggccgccccc ctgccggacc gcctttggtc cctgctcagg caagaaggca ttgagcagct
     1621 ctactcgcac caggccgacg cctacgacgc cgtgcaggcc ggcgagagcg tcgtcgtcgc
     1681 cagcggcacc gcctccggca agagcctgtg ctaccagttg ccaatcctcg ccgccctgct
     1741 gaacgatccc gaggcccgcg ccctctgcct ctaccctgcc aaggccctgg cttacgacca
     1801 gctcggcaat ctcgaacgca tggtcgcgac cggcgaaatc gcggacgtgg ccaagcccgc
     1861 ctgctacgac ggcgacaccc ccacgcacaa acgggccggc atccgccgca gcgccagcat
     1921 cctgctgtcc aaccccgaca tgctgcacca gagcatcctg ccgtaccacc cgaagtgggc
     1981 cggcttcttc gcccggctga aatacgtggt gctggacgag atccacactt accgcggcat
     2041 tttcggcagc cacgtcgccg gcgtgatccg ccgcctgcag cgcatctgcc gccattacgg
     2101 cggcgcgccc cagttcatct gctgctccgc cacgctggcc aacccgcgtg aactggccga
     2161 gcgcctgaca aaccgcccga tgcgactgat cgaccaggac ggctcgccgc gcggccggcg
     2221 ctggttcgtg ctgtggaacc cgccctggct ggatactccg acgcgcatca cgcgccgcag
     2281 cgccaacatc gaggcccaag acctgctgcg agacctgctg gaagaaggcg cgggaaccat
     2341 tgctttcgcc aaggcccgtg tcgtcgccga gctgctatac aagtacgtgg ccgacagcct
     2401 gcgccgccgc cagccggaac tggcccgcaa gctgcgcccc tatcgcggcg gatatctgcc
     2461 gctcgaacgg cgcgagatcg agcgggccct cttcagcggc gaactgttgg gcgtgtgcag
     2521 cacaaacgcc ctagaactcg gcatcgacgt cggctcgctc gatgccgcgc tcatcgtcgg
     2581 ctttcccggg acactatgca gcctgtggca acaggccggc cgggctggcc gacgccagga
     2641 cgaatccctg gcgatcttcg tcgcgtacga tgacccggtc gaccagtacc tgatgcgtaa
     2701 tgcggacttc atcttcaagc gcccgctcga gcaagccatc atcgacccgc aaaacccgca
     2761 cattcttgcc gctcaactgg cgtgcgcggc ccgcgagctg cccctcacac cggccgatct
     2821 gacgaccttc ggcaccctgg ccgagccggt cgccaacgaa ctggtgcaag ccggccggct
     2881 caaggcgggc caagaccgct acgattattc cgtcgcgggt ctgccgcatc acgaaacgaa
     2941 cctgcgaacc atcagcaacg cgacctacgc gatcgtggac ataaccggcg ggcgcaacga
     3001 ggtcatcggc caggtcgatt caatctccgc ccccgaactc gtctaccccg aagcgatcta
     3061 cctgcaccag ggcgacagct acatcgttcg ggccctggac ctgtcggccc gcctggcgcg
     3121 ggtcgagcgc atggacgccg actactacac gcagccggtg ctggcggacg agtgccgcat
     3181 cgtcgccgag cgtctgacgg ccgactgcct gggcgggcgg cgcagcttcg gagacgtgac
     3241 ggtacgttgg caaacggtcg cgttccgtaa attcaagttc cgcaccatgg agctgatcgg
     3301 ccagaccgcg ctcgacctgc cggcccagca ggtgaacacg acgggttgct ggctccagcc
     3361 gccgccagcg gccatcgaag ccacaaacgc ggcgctggcg gctgctgcgg cctcggtgac
     3421 tccaagcccc ggcgccggca taccgagctc gggcgttgct ataccaagcc cgggcgcaag
     3481 ggcggtaccg gacccgggcg cgagggccat accgagcttg ggcatcagcg ccacgctgag
     3541 cccgggcgcg acgctcttcg acgccttcag cggcgtgcgc aacctgctgg ccgtcgccct
     3601 gcccacgctg gccatgtgcg accgctacga catcggcggc gtggttgacg cctcgcagct
     3661 cggcgtgatg acgatcatcc tctacgaccg ctacgaaggc ggcgtcggct acgcccgcca
     3721 cggctacgag tgcgccgccg acctgcttaa gctggcgtat gacatcgtcg ccggctgcga
     3781 ctgcgacaac ggctgcccgg gctgcgtcgg cccgcccaac ctgcgcgtcg cgattcatca
     3841 cgaccccgac ctggggcgcg gctacaagat accagacaaa gcggcgacta tgacgctctt
     3901 gcgtgcctgg ctggacaggt ctgcaagtaa gtagcagata actagcgggt aagtagcaga
     3961 gccggctcgt gccgagccgg aatctgcccg tctcgatccc ttgttccctc gtccctcgat
     4021 cccttcccaa gcaacacgcc ccggctctgc tctggtatgc tttggcgcca tgcttggacg
     4081 aaatcctggt tcagccgtga tcttcgacct cgacggcgtg ctcaccgaca ccgcggagtc
     4141 tcatttccag agttggatgg atgtgtcgcg cgagttcggc atcccgttcg atcgcgccgc
     4201 caatgacgcc ctgcgcggcc tgagtcgcga ggacagcttg cggctgttcc tcggcccgca
     4261 cgcgtcgcgc ttcagtgccg ccgagcaaac gcagatcata actcgcaaga acgaccggta
     4321 tctggcccgc attgagcgta tgagtaccgc cgatttggct ccgggggcgc gcgagctgct
     4381 gcaggacctg cgccgcctaa gcataccggc cgccgtagcc tcatcgagcc gcaatgcggt
     4441 ccaagtgctg gagcgcctgg gcattcggcc gctgctcgac gccgtggtgg atggcaacga
     4501 cgtcccggtt tccaagcccg acccgaccgt gttcctctgt gcggcggaac ggctgggcgt
     4561 acaaccccaa cgctgcgtcg tcgtggaaga cgccgcgagc ggcgtagccg cgggactggc
     4621 ggccggcatg agggtagtgg gcctgggccc gccgcagcgt gtcggccggg cacatctggt
     4681 gctcgatagt ttggctgagc tggatgcgcg gcgcattctg gcgctcctgc cttctgacga
     4741 ctgactcccc cgccaagtca catgttcttg cgaacgctac tatgggcagc ggcttgggca
     4801 acttgaagca ttgatccagg gcgtgccgat gatgcccgcc tgatactgtc tggaaaggcg
     4861 attgggcgcg ggtggattcg aaccaccgta ggcgtaagcc aacgggttta cagcccgtct
     4921 cctttggcca ctcggacaca cgcccgatta acggcgcctg ccgcggaaga cttttccgcc
     4981 gttgcccggc actttgtgcc gtgctcgccg gcaccggctt ccattcgagc cacgaatagt
     5041 acgccatggc ggccattttg gcaactggca aagctgccgc ggcggccggt cgcggcgccg
     5101 gcccttgccc tatgccaccg gggcgtcggc ctcgcccagt gccgcccgtg cgccggccct
     5161 gtttctatgc caagcctttg aactcgtcga gcgcttcggg gttcgccagc gcgtcgcggt
     5221 tgcttacgtc ctggccgtgc agcacctgcc gaaccgccat ttcgaccttc ttgccgctga
     5281 tggtgtgcgg aatcgcactg acctggcgga tcagcttcgg tacgtgccgc ttggtcgcac
     5341 cttcggcaat cgccgtgcga atcttcttct ccagcgcctc gtccagcttc aagccctccc
     5401 gcagcaccac gaacaggcac acctcgacgt ccgcatccgg cgtgtcccgg ccgaccacga
     5461 tgctgtcgac aatctccggc attccctcga ccacgcggta aatctccgcc gtgccgatgc
     5521 gaatgccacc cgggttgagc gtggcgtcgg agcggccgta gacgatcacg ccgccgcgct
     5581 ccgtgatttc gatgaaatca ccgtggcgcc aaatgcaccg gcccgggtag tagtcaaagt
     5641 aggccgagcg gtacttgcgg ccgtcggggt cgttccagaa gcagaccggc tgcgatggga
     5701 aggggcgggt gcaaaccaac tcgccctttt ccccgattac cggccgcccg tcggcgtccc
     5761 aggcctgcac gtccatcgcc aggccggtgc actgaatctc gcccgcatac acgggcagca
     5821 tggggttgcc cagcatgaag caggaaatga tgtcggtgcc gccgctgatg ctggcaagct
     5881 gcacgtcgcg tttgacctca tcgtacgtcc agcggaacaa ctcctcggag agcggcgaac
     5941 cggtcgagca gatgatgcgc aaggcggaca ggtcatgctc gcgccccggc cgcaggcccg
     6001 ccttttggca catgcccagg aatttcgggc tggtgccgaa gagtgtgacg cgcagcttct
     6061 cggcgagttg ccacaagcgc ttcgcgtcgg ggtaggccgg attgccttcg aacaacacga
     6121 ccgcggtgcc gatgccgagc gcgctgacga gccaattcca catcatccag ccgcaggtcg
     6181 tgaagtagaa gctgcgatcg ccgcggcgca gatcgcaatg cagcatcagc tccttcatat
     6241 gctggagcag cgtgccgccg tgaccgtgaa cgatgcactt ggggataccg gttgtgccgg
     6301 aggaatacat gatgaagagc gggtggtcga acggaacttc ttcaaaagtc agcggcggtg
     6361 cggcccggtc gagggggccg agccactcgc gccacagcac ggcgttcggc aggccggcca
     6421 ggctcggctg ttcatccatg aacgggacga ccgcgacgcg ccgcacgccc ttgagctgag
     6481 agagcatttc ggctacccgc ggcagcgtcg ggcagcgttt gccgttgtag gtgtatccgt
     6541 cggtgaccac gagcacgctt ggctcgatct gaccgaagcg gtccagcacg ccgcgcacgc
     6601 cgaaatccgg cgagcaggac gaccacactg cacccaggct tgccgcggcc agcattgcaa
     6661 tcacggtttc gggcacgttg ggcatgaagc ccgcaacgcg gtcgcctttt ttcacgcccg
     6721 cctggcgcag agcagccgca caacgcgcca cctcgtcgcg cagctcccgg tgtgagagcc
     6781 gcaccgcgcg gccgcgctcg tcctcggcca ggatggcgtc ctggtcgtca ttaaagcgga
     6841 gcatgtggcg ggcgtagttc agcgtcatgc cggggaacca cttggtaccc agcatgccct
     6901 cgcccctcat ggtggcgtcg gccggccgcg aaggcttaac ctcggcgaat tcgagcaagt
     6961 cgttccagaa taggtcgcgg cgctcgatag accatcggcg tagcgactgc caatcgggct
     7021 ggacgccgtg cctgctcgcg acggtggtca tgaattggaa catttgcgac tgatgcacgc
     7081 gatcttcaga cggcttccac aacagcgcgc ttgacatggg cgcaacctcc gaaaaaggga
     7141 cgccgctcat tgttgccgtt ggcgccaggc cggtcaacgc cgggcctgga tgtggaaaac
     7201 ccgcggcctt gctcgaagcc ccggccgcag atacaacgac tcaaatcgac cagccagaag
     7261 tgacatcggc ccgcccgcga ccgatgtgga cttgaaaaaa cggccttaac tgtcaatgag
     7321 gcgaggaacc atcatgcaac gcgagtttga ccgtcgagcg tttctgaaat cggccggggc
     7381 tgcagtcggg gcggttagtt tggcgggtgc gctcggattt gcacccgcga cgggcgaggc
     7441 gcctcaagca cagccgccat ctcacgcggg cggccgccgc tgcaagctgc agaaatccct
     7501 gaaatatggc atggttgcag gcggggcgtc ggtcctcgac aagtttgagc tcgccaaaaa
     7561 ggccggcttc gagggtatcg agctggacag tcctaacacg ctggatgcag acgaggttct
     7621 gcgggcccgc gacgtcactg gcctggtgat tgacggcgtg gtcgattcgg tgcattggcg
     7681 gcagacgctc ggcgacccag acccggcagt gcgggccgcg ggacgccagg cgctggagac
     7741 ggcactgcgc gactgccggc tgtacggtgg aaccaccgtg ctactggttc cggccgtggt
     7801 gagcaagaag atttcgtacg ccgatgcgta cgcccggtcg caggaggaaa tccgcaaggt
     7861 actgccgctg gcggcggagc tgcgcgtcac gatcgcgatt gagaacgtat ggaacgcctt
     7921 cctgctcagt ccgctcgaag cggccaggta cacggacgaa ctgaacagtc cgtgggtcgg
     7981 ctggcacttc gacgtaggca acgtcgtcaa ctttggctgg ccggaacagt ggattcgcac
     8041 tctgggcaag cggatagtca aactggatat taaggaatac agccgcaaga agcgcgacga
     8101 gcaggggccg ggcgccgggt ttgcggtcga actgggcgaa ggcgattgcg actggccggc
     8161 agtgatggag gcgctggacg aaatcggttt ccagggctgg gcagccgcgg aagtgggcgg
     8221 cggcgatttg gagcggctaa agaagatcgc gacaactatg gaccgcttgt tcaacagcta
     8281 aatgggttgc ggcgccgatc ctcgaacaga cagccagccg cctgaaaggg cagccggtca
     8341 cgttgcctgc gcctaccgtc ggggcattgc gggcgcagca gagcgagccc gcgggaatgc
     8401 aactgcgaaa atagtgcgct tggccgacgg ccgctattct tcctaattat tccagaaagg
     8461 acggttctct atcgtcagtt gcgagaaccg cggtcctggg cggccgcttg ggtattcgga
     8521 cgccgccgca cgctgcccgc gcggactttg gcccgcttat cacgcgacag gccggttttg
     8581 gtctcagtct tttgagccgt cgctctctgc ggtgcagctt tcgcggccga cgctttccgg
     8641 ccgggcttgc ccacggcagc ttcttgtggt tcgggtacgc ggtcgctgga tgagccgatc
     8701 tcgcccgcgg ttggcgctga gggccacttc cgctcaggca gtacttgtgt gagcacgtcc
     8761 tcgatcgtcc gtgagaaggc aaagatcagt tcgtcgcgca cctctttggg aacttcctgc
     8821 aagtccggct cgttgcgggc cggcagtaca accttcttca gcccggcgcg gtgggcggcg
     8881 agcaccttct ccttgacgcc gccgatcggc agcacctgcc cacgcagcgt gatttcgccg
     8941 gtcatgccaa cgctcgggtc ggcgatttgg ccggcgaaca gcgaagccag cgcacagacc
     9001 atggcgacgc cggccgaggg gccgtctttc ggaacgccgc cggccgggac gtggatgtga
     9061 atgtcggttt tgcccaggtc cgggagcttc aggccccact gcctggcgcg cgaccgaatg
     9121 atcgacaggg cggccaggcc cgactcgcgc atcacctcgc cgatctggcc ggtcagcgcg
     9181 aaattgccgc ggccttccat ggcggtggct tcgacgaaga tgatctcgcc gccaaccggc
     9241 gtgtacgcca aaccggtgac cacgccgggc atcccgcgtt cgagggcgag ttcgcgttcg
     9301 aagcgcggcg gaccgaggta ttccacgagg tcttcgggat cgacggccac atccgcaacc
     9361 ttgttgcggg cgaccttggc ggccagcccg cggcagacgg tgccgatcga gcgttccagg
     9421 ttgcgaacgc cggcctcgcg cgtgtagcgc tcaatgatgg cccgaatcgc gtcgtcggag
     9481 aacgtgacgt tgcggtcggc caggccgttc tcggcgagct ggcgcggcac gaggtagcgc
     9541 ttggcaatct cgaatttctc gaacagcgtg tagccgggca gatcgatgat ttccatgcgg
     9601 tcgcgcaggg ccggggcgac cggatcaatg tagttggcgg tgccgatgaa gagcaccttc
     9661 gacaaatcga acggcacgct gaggtagtgg tccgtgaacg agtggttctg cgccggatct
     9721 agcacttcca gcagcgccgc ggccggatcg ccgcgaaagt cggccccgag cttgtccagc
     9781 tcgtcgagca tcagcaccgg gttgcgggtg ccggccttgc ggatttcctg cacgatgcgc
     9841 cctgggatgg cgccgatgta cgtacggcgg tggccgcgga tgtcggcctc gtcgcgcacg
     9901 ccgccaaggg cgatgcgaat gaagctgcgg cccaaagcgc gtgcgatgct caggccgagc
     9961 gaagtcttgc cgacgccggg tggtccgacg aagcacagga tgggaccctt gctggccggg
    10021 ttgagcttgc ggacggccag gtactcgatg atgcggcgtt tgacgttggt caggccgaag
    10081 tggtcctcgt taaggatggc ctcggcccgg tcgaggtcga ggttgtccgg cgtttccttg
    10141 ttccagggca gctcgcagag ccaggtcagg tagtcgcgaa tcacgccgct ctcgggcgag
    10201 gcctgcggga cgcgttcaag gcgtttcagc tcgcgttcgg cctcctgacg cacggcatcg
    10261 ggcataccgg cggcctcgat gcgtttgcgg aactcctcga tctgccccgc ggtgccatcg
    10321 gtctcgccga gttccttctg gatggcttta agctgctcgt gcaggtagaa gtcgcgttgg
    10381 gtcttatcga tctcgcggcg gacgtcgtcc tggatcttct gcgagaggcg cagcacttcg
    10441 acctggttgt tgaggatggc ggtgactttc ttgagccggt cgacgacgtc gaaggtctcg
    10501 agcagttcct gtttctgcac gacgcccagc gagaggttgg cggccaggaa gtcggccagg
    10561 gcgtcggggc ggtcgatgtt gtcgaggacg aggcgggcct cgtcgggcac attggggctg
    10621 agctcgatga cctcgcgcgc gatctggcgg gcgttgtgca ccagggcctc gagttcgagc
    10681 gacggggtga cctgatcgat atgcgggttg accacggccc gccaatacgg gtcggtagcc
    10741 accagttcct cgatgccgac gcgtaccagg ccatgcacga gcaggctgtt cgagccatcg
    10801 ggcagtttga ggactttgag cacctgggcg gcggtgccga tgcggtagag gtcatccagg
    10861 ccggggtctt cgacctcttc gtggcgttgg gtgaacaggg cgagcagctt gtcgcccgcc
    10921 aggacggcgt cggtgagcag gcgtgacttc tcgcggccga tgctgagcgg aacgacggtg
    10981 ccggggaa
//
LOCUS       DAOSYS010000116        10757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5232_length_10757_cov_5.453373, whole genome shotgun sequence.
ACCESSION   DAOSYS010000116 DAOSYS010000000
VERSION     DAOSYS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5232_length_10757_cov_5.453373"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLU99_08585"
     CDS             complement(<1..244)
                     /locus_tag="PLU99_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17152.1"
                     /translation="MTLDRRRARLWPAAVAIVLLTASAALCADEHPLLSCMQEELQLS
                     LENLVGSDGTKPYFLQYSVYDEQEVFVSASLGALTAD"
     gene            461..637
                     /locus_tag="PLU99_08590"
     CDS             461..637
                     /locus_tag="PLU99_08590"
                     /inference="COORDINATES: protein motif:HMM:NF018946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HOO17153.1"
                     /translation="MPTQAGEIAKKTADLICSKCGGQMHVTQGVKVPKCTCGNDTFVE
                     RRQEPSTAASEKRR"
     gene            826..2067
                     /locus_tag="PLU99_08595"
     CDS             826..2067
                     /locus_tag="PLU99_08595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO17154.1"
                     /translation="MPDAPEDSQILKTVVERQLVTPHEVRTAVDYHKKILAGGRRRPM
                     SEVLVDLGFLTRTQLNRLQGVNDDSGGQPAQQIPGYQLLNKCGAGAMAVVYRAKQVSL
                     DRIVAVKVLPRRLSKNAEFVERFYREGRAAAQLNHNNIVQAIDVGEAGGYHYFVMEYV
                     EGCTVYDELAEGKVYEEPEAIDIITQVARALQHAHERGIIHRDVKPKNIMLTKDRVAK
                     LADMGLARETSDNEAAMAEAGRAYGTPYYISPEQIRGEVHIDHRADLYSLGATWYHMV
                     TGRVPFEGATPSAVMHKHLKEPLTPPDHLNTRLSAGCGAMIETLMAKNRDQRYSSARE
                     LLTDLALLARGEPPYHANRNLADDDLGSLTAGDAVPQEQLVVSRKGGRDRHDTAVTLA
                     IVLGAVAAISVLGNLIQLAVG"
     gene            2136..4100
                     /locus_tag="PLU99_08600"
     CDS             2136..4100
                     /locus_tag="PLU99_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17155.1"
                     /translation="MLWAVFCIVLAGASLLSFWPCLANDFNYDDTAFLLENPHYRGLG
                     ATQLRWMFSTFHFGHYQPLTWISLGLDYLLWGMNAWGYHLTSLLLHCANAILVYWLAL
                     SLMRRAAERGGAARHVASWHLPASALVAALVYSLHPLRVETVAWVTDRADVLSTFFLL
                     LCTLSYLRAARAGTRMSRAGWLTLAVMLYAAGLLSRGMGVPLPLVLLLLDWYPLGRLG
                     SRSTSRLPWPVLLEKIPFVILAIAAAVMAPIAKATQGLTVSLAEHGVVARLAQAAYGL
                     VFYIYKTVVPTGLVPIYELRLPLDIVSLRYLGPLFLVLAAGVVLIRYWRRWPAVTVAL
                     LSYAILISPLLGLVQAGTQEVADRYSYWPAISLAILLGGGCFGLLCRSQSRNWARRLL
                     FAVSGAVVVLLGVLSWRQSLVWRSEESLWTHAVRNGPVSSAAHYNLGYVYANHGRVAE
                     AMAQFDMGRRINRQWSLARLMAGQALARAHAWPEACQIFGDLLALEPEHAVAATGLGE
                     ALTALQRPAEAEAAFRQALRTAPSAADAWLGLGNALVAQRRLDEAAEALRQAVRFAPQ
                     RASANGNLGLVLAQLSRFDEAETYLRRAVELDPDYFEGYLNLANLLQIRQRPEEAARV
                     LGTALRLRPDHPEAQRRLTILNQQRGAARP"
     gene            complement(4106..4966)
                     /locus_tag="PLU99_08605"
     CDS             complement(4106..4966)
                     /locus_tag="PLU99_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOO17156.1"
                     /translation="MASASTRFDPADQALLRQVRALAEEAAQIGGEIARAAFSGNFTV
                     RRKADGTEVTSADEDAQAAIVGFLRSRRADDAFITEEDSAPAGAGAPTPVTNDVLCWV
                     IDPLDGTRNFVHGVPLYAVSVAAMFAGVPVVGAVYLPERGELFSAGGPDGFLIDRRPP
                     PRRPPLPPRSGGRPPKPLVGIPSSLQGGTNTVIQSWTGRVVIRNTGATAMHLAMLGAG
                     QLQATLISDSRLWDIAAGWLLVTSTGGVMTALDGRDLFPLDVSRYAGEAMPSLASANP
                     ELHADLLPKP"
     gene            complement(4966..7155)
                     /locus_tag="PLU99_08610"
     CDS             complement(4966..7155)
                     /locus_tag="PLU99_08610"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HOO17157.1"
                     /translation="MDRNQFRTLVTGLLLALGVFIAYQYVISWVYPPRKPPAPGEPAA
                     VAPAPPGATQPGAGEPSGTQPSFAEAAPPATATAPTAEQFHFVRGSSLNNIVLGGAPR
                     YNVQLSFTSRGAAVEWIRLTTRNEEGKYVYNSAPGVNEPYQLIKPVQDSETYDSFATA
                     EIQIREMGDHTWRLADLPWIVSAQTDESIAFTTTLQQIQGDERLVRLTKTYTLVPEKP
                     LVDLSLTAENLSTRPLTIKLTQDAAVGIPREGPSTDMRAVMAAKMADGVVQTAKVETR
                     NNLRTGAGEPNTVTLFTATAESEFAWTALVDRFFGVFVRPLRQPESPERVVQVETTAV
                     APQMEENPGDMLARMITAPLTLVPQQQAVYKFEIYAGPKAPDRLAEVNPAFTDKTQVY
                     YSLAQAAAGGGCACTFQPLPQIMVGLLHAIYYVVPNYGIAIIILVLIIRTLLHPLTVY
                     QQKSMYRFQEKQARLQPRIEAIKAKYPNDRARQQQEMMKVYNEEGVNPAGMLVNMLPM
                     LIQLPILVALWTGLNTDIALRHAPFDGYWIKDLAAPDALIKFSPPISIPILAQLPWLG
                     RMFQNIPALNLLPILMGVAMWLQQKYMPKPGMQAKADAAKQRPPAERRPGQMSPEDQL
                     RQQRIMMYMMTFIFPLMLYYMPSGLNLYWMATNVFGVFESLVVRKQIEEEKQRAQREG
                     PKPPPKPGWLSRKWKALVEQAAEIQKRADELQREQGKGRRDKGGKNR"
     assembly_gap    7390..7489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7490..8369)
                     /locus_tag="PLU99_08615"
     CDS             complement(<7490..8369)
                     /locus_tag="PLU99_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO17158.1"
                     /translation="MAIGHAQARQWPGVVALVICCALWSLNGPLIKLLNEQHVPGVTV
                     ACFRSLIGGLVFLPFAWGQRGQLRKVSAAWPIGSVLMFTLMTVSFVISTMMTTAANAI
                     ILQNTSAIWVFLLAPLLLKEKPGRVEGLVLLLAMAGIAVIFFGGRETGLTGLVIALIS
                     GFGYGALIIMLRGLRSVNALVVTAMNSLGSGLLLIVPTLIWGTVLLSPRGWALILTLS
                     LVQFTLPYMLFSWGLQRVEAHRAALIVLLETVLNPIWTFLLVGERVGTPTLIGGPLIL
                     LGVAGWLLLSARQRSPA"
     gene            complement(8447..9028)
                     /locus_tag="PLU99_08620"
     CDS             complement(8447..9028)
                     /locus_tag="PLU99_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOO17159.1"
                     /translation="MNLLRRAFLLAAPLTLAFGCGRFHGLDLGLLTPLDNFHTVEAGK
                     LYRSAQLGKHTLKAVCQKMGIRTVVNLRGQNSGSSWYDDEIACCAAIGVKLVNIPMES
                     DEMPTREALLELWDTFENGEYPMLVHCKAGADRTGGAVAIWRMMEGESRQAAARELSV
                     AYGHSQAMYPEWDRLLGMFRPSREWIEHEYPVQ"
     gene            complement(9158..10375)
                     /locus_tag="PLU99_08625"
     CDS             complement(9158..10375)
                     /locus_tag="PLU99_08625"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOO17160.1"
                     /translation="MPILVQKFGGTSVADAYRIHLAARRAVRALLDGNQVVMVVSAMG
                     HTTDHLVGLARQVTRRPSKREMDMLLSTGEQVSIALVAMAIHEAGYDAISLTGAQIGL
                     LTDSRHTEARIQTINTERIRRQLDQGRIVIVAGFQGVDPDFNITTLGRGGSDTTAVAL
                     AAALGAAACEIYTDVPGVYSADPHIVPRARKLDQIGYDEMLELAALGAGVMHPRSIEL
                     GMKFSVPIHVRSSFSEEPGTMIMSETRGLEEIVVRGCTLKPDIGRVTLTGLPNEPGIE
                     NTLFEPLARRHIIVDDIIQLVAPGGQTVSLSFTVSGSDLGEAQALAETIAGRYAGVQF
                     AIQKDLAKLSAVGAGMRSHSGVAATMFAALAAERINIENISTSEIVISVLVRAADADR
                     ALLTVHRAFGLEA"
     gene            complement(10392..>10757)
                     /locus_tag="PLU99_08630"
     CDS             complement(10392..>10757)
                     /locus_tag="PLU99_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17161.1"
                     /translation="RILVVEDHAGLARLMGLALESLGHAVRTAGGVNAALELAAQEQF
                     DLLVSDLGLPDGSGLDLMRQLAGRQIKGIAFSGYDDEEHIRAARAAGFAAHLKKPVEM
                     EDLAATIERVLSQSESPKP"
BASE COUNT         1911 a         3369 c         3383 g         1994 t
ORIGIN      
        1 agtctgccgt aagcgctccc agactggctg agacgaacac ctcctgctca tcgtaaacgg
       61 aatattgcag gaaatacggc ttggtaccgt ctgaaccaac caggttctcc agcgaaagct
      121 gaagctcctc ctgcatacac gaaagcaaag gatgctcatc ggcacacagg gcggctgaag
      181 ccgttagtag cacgatcgca acggctgccg gccagaggcg tgcgcggcgg cggtctagtg
      241 tcatgtgcgg gccctcgttg tcgcttttcg gctcggcagg gcgttaggca ttgtacccgg
      301 ccttacaaat ctgaaaaccc gttgccattt cgacctttaa agacatttga gaccacattc
      361 aggctctgtt caccggtctg aggttcaaaa tcggttgcga ccgaggaggc ccgaagcgtt
      421 ttggggccgg tcgcgcgcga atttcaaaag ggtggtcgag atgcctacac aagctggaga
      481 aattgcgaag aagacggccg acttgatctg ctcaaaatgc ggcggccaaa tgcacgtgac
      541 tcagggcgtc aaagtgccga agtgtacatg cgggaacgat acctttgtgg agcgtcggca
      601 ggagcccagc acggcagcga gtgaaaaaag acgctgatcg acgcttccgc atattcggac
      661 ccacggagcc gcgagacgct tacgagggcg gccaggcccg gtgcaccggc tgccggcggt
      721 gccccgggcc cctatggttt gaatccgccg attattttgc gtagaatttg acgaaaattg
      781 cggcggaaac tgtcggcgag cagacccata atcatagaga agaccgtgcc cgacgccccc
      841 gaagacagtc agattctgaa aacggtcgtc gagcgtcagt tggtgacgcc gcacgaagtt
      901 cgtacggccg ttgactacca caagaaaatc ctcgccgggg gccgtcgcag gccgatgagc
      961 gaagtcctcg tcgacctcgg ttttttgact cggacccagc tcaaccggct gcagggggtc
     1021 aatgatgact cgggtgggca gccggcgcag cagattcctg ggtaccaact gctgaacaag
     1081 tgcggggccg gggcgatggc cgtggtttat cgggccaagc aggtcagcct cgaccgtatc
     1141 gtcgcggtga aggtcttgcc ccgccgtttg agcaagaatg cggagttcgt ggagcgcttt
     1201 taccgcgaag gccgcgccgc tgctcagctc aaccataaca atatcgttca ggccatcgac
     1261 gttggcgaag ccggcggcta ccactatttc gtgatggagt acgtggaggg gtgcacggtc
     1321 tacgatgagc tggccgaggg caaagtatac gaggagcccg aggcgatcga cataatcacg
     1381 caggtggcgc gcgccctgca gcatgctcac gaacgcggca tcattcaccg cgacgtaaag
     1441 ccgaagaaca tcatgctcac caaggatcgc gtggccaagc tggcagatat gggtctggcc
     1501 cgcgaaactt ccgataacga ggcggccatg gccgaagccg gccgcgccta tggcacgccg
     1561 tactacatca gccccgagca gatacgcggc gaagtgcata ttgatcatcg ggccgacctg
     1621 tattcgctgg gcgccacctg gtaccacatg gttaccggtc gtgtgccgtt cgagggcgcg
     1681 acgccctcgg ccgtcatgca caagcacctg aaagaaccgc tgactccgcc cgatcatctg
     1741 aacacgcggc tgagtgccgg ctgcggcgcc atgattgaaa cgctgatggc caagaaccgc
     1801 gaccagcgct actccagtgc ccgcgaactg ctcaccgatc tggcactgct ggcccgcggc
     1861 gaaccgccct accacgccaa tcggaacctc gcggacgatg acctcggaag ccttacggcc
     1921 ggcgacgccg tcccccagga gcagctcgtc gtgagccgca agggcggccg cgaccgccac
     1981 gacacggccg tcactcttgc gattgttctg ggtgctgttg cggccatcag cgtcctgggc
     2041 aacttgatcc agctcgcagt aggatgagag cgcgagtccg agcgggcgtt ctcggggagg
     2101 atacttcagg tgcagtctgg gcgcggggcg gcggagtgct ttgggctgtt ttctgtattg
     2161 ttctggccgg tgcatcgctg ctgagcttct ggccgtgtct ggccaatgac tttaattatg
     2221 atgacacggc cttcctgctg gagaatccgc attaccgcgg gctgggcgcc acacaactgc
     2281 gttggatgtt ctcgacgttc cacttcgggc attaccagcc gctgacctgg atcagtttgg
     2341 ggctggatta cttgttgtgg gggatgaatg cctggggcta tcacctaacc agtctcctcc
     2401 tgcactgcgc aaacgccata ctggtctact ggctggcact gagcctgatg cgccgcgcag
     2461 ccgagcgcgg cggcgcggca cgccacgttg cctcctggca tctgccggcg agcgctcttg
     2521 tggccgcact cgtttatagc ctccatccgc tacgggtaga gacggttgcg tgggtgaccg
     2581 accgggccga cgtgctgagc acatttttcc tgctgctgtg tacgctgagc tatttgcgcg
     2641 cggcgcgggc cggcacacga atgagccgcg ccggctggtt aacgctggcc gtgatgctgt
     2701 atgcggccgg gctgctcagt cgcggaatgg gcgtgccatt gccgctggtg ctgctgttgc
     2761 ttgactggta tccgctgggc cggctcggct cgcgctcgac gtcgcgcctg ccatggccgg
     2821 tattgctgga gaagatacca tttgtcattc ttgcaatcgc cgctgccgtt atggcgccca
     2881 tagccaaagc aactcagggg ttgacggttt cgttggcgga acacggcgtt gtggcacgcc
     2941 tggcccaagc cgcttacgga ctagtcttct atatctataa gaccgttgtg cccacgggac
     3001 tggtgccgat atacgagttg cggctgccat tggacatcgt ttcgctgcgt tatttggggc
     3061 cgttgttcct ggtgctggcg gcgggcgtgg tactcatacg ttattggcgg cgctggcctg
     3121 cagtgactgt cgcgctgctc agctatgcca tactgatttc gccactgctc gggttggtgc
     3181 aggccggcac acaagaggta gccgatcgat actcgtactg gccggcgatc agtctggcca
     3241 tactcctggg cggcggttgc tttggcctgt tatgccgcag tcaaagccga aactgggcgc
     3301 ggcgactgct gttcgccgtc tctggcgcag tggtcgtact gctgggagtt ctgagctggc
     3361 gacaatcgct ggtatggcgc agcgaagagt cgctctggac acacgcggtt cggaacggcc
     3421 cggtttcttc tgccgcgcac tataacctcg gatatgtcta cgcgaaccac ggccgcgtgg
     3481 ctgaggcaat ggcgcaattc gatatggggc ggcggatcaa tcgtcagtgg agccttgcgc
     3541 ggctgatggc cgggcaggcg ctggcccgcg cacacgcctg gcccgaggcc tgccagatat
     3601 ttggcgactt gcttgcgttg gagccggagc atgccgtagc cgcaacgggc ctgggagagg
     3661 cgctgacagc tctgcagcgg ccggcggaag cggaggccgc atttcggcag gcattacgca
     3721 ccgcgcctag cgccgctgac gcctggttgg gattgggcaa tgcactggtc gcacagcgcc
     3781 gcttggacga agcagccgag gctctgcgtc aggcggttcg gtttgcaccg caacgcgcga
     3841 gtgcaaacgg aaacctcgga ctggttctgg cgcaactgtc acgctttgat gaggcggaaa
     3901 cctacctgcg acgcgcggtc gaactggatc ccgactactt tgaaggatat ctgaacctcg
     3961 ccaatctgct gcagataagg cagcgcccgg aggaggccgc ccgtgtcttg ggcaccgcct
     4021 tgcgcttgcg gccggaccac cccgaagccc agcggcgact gacgatcttg aatcagcagc
     4081 gcggggccgc acggccgtaa gcgttttacg gcttcggcag gaggtctgcg tgcagctcgg
     4141 ggttcgcgga ggcgagcgag ggcattgcct cgccggcgta gcgggaaacg tcaaggggga
     4201 acaggtcgcg cccatccagc gcggtcatga cgccgccggt cgatgtcacg agcaaccaac
     4261 cggccgcgat atcccatagc cggctgtcgg atatcaaggt ggcctggagc tggccggccc
     4321 ccagcatcgc caggtgcatg gcggtcgcac cggtattccg tatgacgacg cgccccgtcc
     4381 agctttgtat cacggtattc gtgccgccct gtaacgagct gggaattccg accagcggct
     4441 tgggcgggcg gccaccggag cgcggcggca gaggcgggcg ccggggtggc gggcggcggt
     4501 cgatgagaaa gccgtctgga ccgccggcgg aaaagagctc tcctcgttca ggcaagtata
     4561 cggccccgac taccggaacg ccggcgaaca tcgcggccac cgagaccgcg tacagcggga
     4621 cgccgtgaac aaaattgcgc gtgccatcga gcgggtcgat cacccagcac aggacgtcat
     4681 tcgtaaccgg cgtcggcgcc cccgccccgg ccggtgctga atcctcttca gtgatgaagg
     4741 cgtcatccgc ccgccggctg cgaaggaacc ccacgatggc ggcctgcgcg tcctcgtcgg
     4801 cgctggttac ttccgtgccg tcggccttac ggcgaacggt gaaattacct gaaaaggcag
     4861 cccgcgctat ctccccaccg atttgagcgg cttcttcggc caaagcccgt acctgccgga
     4921 gcagggcctg gtcggcaggg tcgaagcgcg tgctcgcact ggccattaac gattcttgcc
     4981 tcccttgtcg cggcggcctt tgccctgctc gcgctgcagt tcgtcggccc gtttctggat
     5041 ttcggccgct tgttccacga gcgccttcca ctttcgtgac agccaacccg gcttgggcgg
     5101 cggcttcggc ccttcgcgct gggcgcgctg cttctcctcc tcgatctgct tgcgaacgac
     5161 gagcgattcg aaaacgccga agacgttggt ggccatccag tagaggttca gacccgaggg
     5221 catgtagtag agcatcagcg gaaagatgaa cgtcatcatg tacatcatta tccgctgctg
     5281 gcgcaactgg tcctctggcg acatctgccc cgggcggcgt tcagcaggcg ggcgctgctt
     5341 ggccgcgtcg gccttggcct gcatgccggg cttgggcatg tacttctgct gcagccacat
     5401 ggccacgccc atgagaatgg gcaagaggtt cagggccggg atgttctgga acatacgccc
     5461 caaccacgga agctgggcga gaatggggat cgagattggg ggcgagaact tgatcagggc
     5521 gtccggggcg gccaggtcct tgatccagta accgtcgaac ggcgcgtgcc gcagggcgat
     5581 gtcggtgttc aggccggtcc agagcgcgac cagaatgggc agttgaatga gcatgggcag
     5641 catgttcacc aacatgcccg ctgggttaac gccctcctca ttgtagacct tcatcatctc
     5701 ctgctgctgc cgggcgcggt cgttggggta cttggccttg atcgcttcga tcctgggttg
     5761 caggcgggcc tgcttctcct ggaagcgata catcgacttc tgctggtata ccgtaagcgg
     5821 atgcagcagc gtgcggatga tgagcaccag aatgatgatg gcgatgccat aattcggcac
     5881 aacgtagtaa atcgcgtgca gcaggccgac catgatctgc ggcagcggct ggaaggtgca
     5941 ggcacacccg ccgccggcgg ccgcctgggc gagcgagtag tacacctgcg tcttgtcggt
     6001 gaatgccgga ttcacctccg ccaggcgatc gggcgccttc ggcccggcgt aaatctcgaa
     6061 cttgtagacc gcctgctgct gcggcaccag cgtcagcggc gccgtgatca tgcgggccag
     6121 catgtcaccc gggttttctt ccatttgcgg tgcgacggcg gtggtttcga cctgcaccac
     6181 gcgctccggg ctttcgggct gtcgcagggg acgcacgaag acgccgaaga agcggtcgac
     6241 cagagcggtc caggcgaatt cgctctcggc ggtggccgta aacaacgtga ccgtgttcgg
     6301 ctcgccggcc ccggttcgca ggttgttgcg ggtctcgact ttcgccgtct gcacgactcc
     6361 gtctgccatt ttcgccgcca tgacggcgcg catgtcggtg gacggcccct cgcgcgggat
     6421 tccgacggcg gcgtcctgcg tgagcttgat ggtcagcggg cgcgtgctga ggttttcggc
     6481 ggtcaggctc aggtccacca gtggcttttc tgggaccagc gtgtaggtct tcgtcaggcg
     6541 gaccagccgc tcgtcgcctt gaatttgctg cagcgtggtg gtgaatgcga tcgactcgtc
     6601 ggtctgcgcc gagacgatcc acggcaggtc ggccaggcgc caggtgtggt cgcccatctc
     6661 acgaatctgg atttccgccg tggcaaacga atcgtaagtc tcgctgtctt gaacgggctt
     6721 gatgagttgg tacggctcat tcacgcccgg ggccgagttg tagacgtact ttccctcctc
     6781 gttgcgcgtc gtgaggcgga tccattcgac cgccgccccg cgcgaggtga atgacagttg
     6841 cacgttgtag cgcggtgcgc cgcccagaac aatgttgttc aggctgctgc cgcggacgaa
     6901 gtggaactgc tcagcggtgg gcgcggttgc cgtcgcggga ggcgcggcct cggcgaagct
     6961 cggctgggtt ccactaggtt cgccggcccc gggttgggtt gcgccgggag gggcaggcgc
     7021 gaccgcggcc ggctcgccag gggcgggcgg ctttcgcggc gggtagaccc acgagatcac
     7081 gtattgatag gcgatgaata cgcctagcgc gaggaggagc cctgtaacga gggttctgaa
     7141 ttggtttcta tccattctga ctcataaccc gggccaattg gctgtagtgg tccggaggct
     7201 agctcgagtt cagtcgttcg gccaagtcga tcggccaact caaatcgtca ggccatctcg
     7261 agttcaagtc gttcggccaa gaacctgctg tccgcgccgg tgcgtgcggc cctcgggcgg
     7321 gcgcgcttca cggcgccggt cgggcaaaca gaaccgcgca ttttattggg actaggcgtt
     7381 aatgcaatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgccggagat
     7501 cgttgccggg cgctcaatag cagccagccg gccacgccga gcaggattag cggtccgccg
     7561 atcaaggtgg gcgttcccac tcgttcgccc accaacaaga acgtccagat tggatttaac
     7621 accgtctcca gcagcacgat cagcgcagcc cggtgggcct cgacgcgctg caaaccccac
     7681 gaaaacagca tgtacggcag cgtgaactgc accaagctga gcgtcaggat aagggcccag
     7741 ccgcgcggcg aaagtagaac cgttccccag atcagcgtcg gcacgatcag caacagcccg
     7801 gagcccagcg agttcatggc cgtcacgacc agcgcgttca cgctgcgcag cccgcgcagc
     7861 atgataatca gcgctccgta tccgaatccg ctgattagcg caatcaccag tccggttagg
     7921 ccggtttcgc gcccgccgaa gaagatcacc gcgattcccg ccatcgccag gagcagaacc
     7981 agtccctcca cgcgccccgg cttttccttc agcagcagcg gtgccagcag gaatacccag
     8041 atcgctgacg tgttctgcag gatgatcgcg ttggcggcgg tcgtcatcat cgtcgagatg
     8101 acgaacgaaa ccgtcatcag cgtgaacatc agcacggatc cgattggcca ggccgccgat
     8161 actttccgca actggccccg ctggccccac gcaaacggca gaaataccag cccgccgatc
     8221 aacgaccgga agcacgctac cgtcacccca ggcacgtgct gctcgttcag cagcttgatc
     8281 aacggcccgt tcaggctcca tagcgcgcaa cagatcacca gcgcaaccac tcccggccac
     8341 tgtcgcgcct gagcatgtcc gatcgccatg gccagtttat accccgctgc ggcgccggcg
     8401 tcccgccggg gcgcatgaat ctacgactgt cgctcccctt ccctgcttat tgcaccgggt
     8461 actcgtgctc gatccactcc cggctcgggc gaaacattcc cagcagccgg tcccattccg
     8521 gatacatggc ttgcgagtgc ccgtacgcga ccgacagctc gcgcgcggcc gcctgccggc
     8581 tctctccctc catcatgcgc cagatggcga cggccccacc agtgcggtcc gcgccggctt
     8641 tgcagtgtac gagcatggga tattcgccat tctcgaaggt gtcccacagt tcgagcaaag
     8701 cttcgcgcgt cggcatctca tcggattcca tggggatgtt cacgagcttg accccgatcg
     8761 ccgcgcagca ggcgatctcg tcatcgtacc acgacgatcc cgagttttgg ccgcgcaggt
     8821 tcacaaccgt acggatcccc atcttctggc agacggcctt cagcgtgtgc ttaccaagct
     8881 gcgctgagcg atacagcttc cccgcttcga ccgtgtgaaa gttgtccaga ggcgtgagca
     8941 ggcccaggtc gaggccatga aatcgcccgc agccaaacgc cagcgtcagc ggcgcagcca
     9001 ggagaaacgc gcggcgaagc agattcatta cgcgactttc ggccctggcg cggctcaccg
     9061 cacggcaggt gacgtgaatg taagctcgac gcgcgccgcc gacaaggggg gagggcccag
     9121 cgcactacgt gctctcaaac gcttcgcgga cgcaccctta cgcttccaac ccgaaggccc
     9181 ggtgcaccgt cagcagcgcc cggtccgcat ccgccgcgcg caccaggacg ctgattacga
     9241 tttcgctcgt ggagatgttc tcgatgttga tccgttccgc cgccagcgcc gcaaacatcg
     9301 tcgccgccac gccgctgtgc gaccgcatgc cggctccaac cgcgctgagc ttggccaggt
     9361 ccttctggat cgcgaactgc acgccggcgt agcggcccgc gatcgtctcg gccaaagcct
     9421 gggcctcgcc caggtcgctg ccggagacgg tgaacgacag gctcaccgtt tgaccgcccg
     9481 gcgccacaag ctggatgatg tcatcgacga tgatgtgccg ccgcgccaat ggctcgaaca
     9541 acgtgttctc aatgcccggc tcgttcggca atccggtcag cgtcacgcgc ccgatgtccg
     9601 gctttagagt gcagccgcga acgacgattt cttccaggcc ccgtgtttcg ctcatgatca
     9661 tggttccggg ttcttcgctg aaactgctgc gtacgtgaat gggaacactg aacttcatgc
     9721 ccagttcgat cgaacgcggg tgcatgacgc ccgccccgag cgcggccagt tcgagcattt
     9781 cgtcatagcc gatctggtct aacttgcggg cgcgcggcac tatgtgcggg tcggccgaat
     9841 agacgccggg aacatccgta tagatttcgc acgccgcggc cccgagcgcg gccgccagcg
     9901 ctaccgcggt cgtgtcgctg ccgccgcggc caagagtagt aatattgaaa tccgggtcga
     9961 cgccctgaaa accggccaca atgacgatcc gcccctggtc gagctgccgc cggatacgct
    10021 ccgtattgat ggtctgaatg cgggcctcgg tatgccggct gtcggtaagc aggccgatct
    10081 gggcgccggt gaggctgatc gcgtcatagc cggcttcgtg gatggccatc gcaaccaggg
    10141 cgatgctgac ctgctcgccg gtcgagagca gcatgtccat ttcgcgcttg gaggggcgcc
    10201 gcgtgacctg ccgcgccagg ccgaccaggt gatcggtcgt gtgacccatg gcggaaacga
    10261 ccatgacgac ctgattgccg tcgagcagcg cccgcaccgc ccgccgcgcc gccaggtgga
    10321 tgcggtaagc atccgccacg ctggtcccgc cgaacttctg aaccaggata ggcatgccag
    10381 taagtcacca gctacggttt tggcgactcc gattggctta gcacacgctc tatagtcgcc
    10441 gccaaatcct ccatctccac cggcttcttc aaatgggccg caaagcccgc ggcgcgcgcc
    10501 gcgcgtatgt gctcctcgtc atcataaccg ctgaaggcga tgcccttaat ttgacgcccc
    10561 gcaagctgcc gcatcaggtc cagtcccgag ccatccggca agcccaaatc gctcaccagc
    10621 agatcgaatt gctcctgcgc ggccagctcc agcgccgcat tcacgccccc cgcggttcgg
    10681 accgcgtgcc ccagggattc caacgccaga cccatcaagc gcgccagtcc ggcatggtcc
    10741 tccaccacca ggatccg
//
LOCUS       DAOSYS010000117        10706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5266_length_10706_cov_5.465402, whole genome shotgun sequence.
ACCESSION   DAOSYS010000117 DAOSYS010000000
VERSION     DAOSYS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_10706_cov_5.465402"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLU99_08635"
     CDS             <1..573
                     /locus_tag="PLU99_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17162.1"
                     /translation="NAKSLLGILMLAAERGARGGGLGRALGEVLLESQAPHKELSLFA
                     TAARVGIPATVHVAVGTDTVHVDPGLDGAALGRATLADFHKVCAVVADLGARTAGGAG
                     GVWLNVGSAVVLPEVFLKAVSVARNLGHSLEGMHTANFDMLRHYRPTQNVVGRPVAPG
                     FGHTVIGQHEILLPLLRQAVVEGLAGGEVT"
     gene            570..968
                     /locus_tag="PLU99_08640"
     CDS             570..968
                     /locus_tag="PLU99_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17163.1"
                     /translation="MKPENASGLFIPEAPDGPAAVEVEEREGCRERDERRGSRTELLA
                     TLERLERTLDEMRGGLEARVRLERHREFSPARAVGVVVQVLVVGLELWALSDWVFGEY
                     GQILYKLSFAGVLQVGALTAFLMGRPAERG"
     gene            complement(993..1349)
                     /locus_tag="PLU99_08645"
     CDS             complement(993..1349)
                     /locus_tag="PLU99_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO17164.1"
                     /translation="MDLRLDEVNGIKVLRIQGDITEDRDSLVVTFTDMLSTPHARIVL
                     DLTRAKYLNSAALGDLVRMVGQANVQEARVILAGAPPFIAGLFAATRLDRFFEQAPSL
                     EDALQRLSTPQPVQPV"
     gene            complement(1471..2772)
                     /gene="larA"
                     /locus_tag="PLU99_08650"
     CDS             complement(1471..2772)
                     /gene="larA"
                     /locus_tag="PLU99_08650"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOO17165.1"
                     /translation="MRVEFNYPNYRDIPPCEIPDDRLIGVYRPQALPDIDEPRVLEAG
                     FQLPYEAPVLNRAVGPHDRVLIIIDDASRGTPVAKILPYVLAELAAAGTPDDHVQFLT
                     AQGTHRRMTEDELRHKLGELHGRFEIHQHDWKDESLMHEFGRTKDGTRVTANRLLAQA
                     DFVLGIGSIVPHRVKGFSGGAKIIFPGVSGKEMMYRNQWEASMRLSETVMGEPENSMR
                     LRMEEAAQIAGLRYIVNVVSDADGKIVGCFVGHPVVAHRAGCALSREVFMVRLPQRAD
                     IVLIDAYPADRDFWQSAKAAYAGTMAVREGGTLITVAPNPEGVADNHPNLMQIGYRHQ
                     AEIVQMVQRGEIEDLVGAAILSDVAQIIDHADCIMVSPGVRPDEAERLGFRYANNCQD
                     ALAMAFQRQGRHARVAVLHQGGHILPATSTCDSHGAQRIFH"
     gene            complement(2801..4000)
                     /locus_tag="PLU99_08655"
     CDS             complement(2801..4000)
                     /locus_tag="PLU99_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOO17166.1"
                     /translation="MSRDEQRVQAIRAALAQEGLDGLVCSLPMHVLLISGYWPVVGTS
                     VAVITRDGVVGVIAPEDERDLAERGWADQLRVFHPGSLDCMTNAVQALREPLSALLAA
                     LGLAHARMGYEDDQSYQPVTYAAMHLYGGALPRLMKEVAPQVELAPASGLLVQLAAVQ
                     TPSEVERVRLACKIVGEAFNYGADHVTVGTSESRTAALFRDQLDSGGDSHDIARSGGF
                     VWCMSGPNSAAACGAYARSRQRPLARGDLVLVHCNSYVDGHWTDVTRTYCLGQPDERQ
                     RAMFAAVAEARQAALAAIRPGVKAADVDKAARSVLQARGFGDAFKHSTGHGVGFSAIN
                     ANARPRLHPKSDDVLQTGMIFNVEPAVYFDGYGGLRHCDVVALTDRGPEVLTPFQGDM
                     QQLWLKV"
     gene            4232..7381
                     /locus_tag="PLU99_08660"
     CDS             4232..7381
                     /locus_tag="PLU99_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOO17167.1"
                     /translation="MANLTVLRRQMEGQRFERQLSEERTAAAERVDIRGLENDLRRSV
                     RGEVRFDKGARALYATDGSNYRQVPIGVVIPRDVEDVIAAVDVVRQYGAPLLSRGGGT
                     SLAGQCCNVAVVFDYSKYMHHVIAIDADRKLARVEPGCVLDRLRKAAAPYKLTFGPDP
                     ATHSHCTLGGMLGNNSCGVHSLLSANAGFGLRSSDNTHALDILTYDGVRMQVGETPPA
                     ELEAAIRAEGRRGEIYAGLKNLSEKYADEIRRRFPKLPRRVSGYNLDELLPERNFHVA
                     RALVGTESTCVVCLEATVHLVHNPPAKSLLVLGYPDIFSAGAHVMEILEHKPTGLEGV
                     DHLLYQWLQEAGKEEANLRLLPEGGGWLLVEFGGDTRAEADEKARATMEMLKGQPQPP
                     SMKLFDDPREEEMVWKVRESGLGATAWVPGRPDNWPGWEDSAVPPEQVGPYMRDFKAL
                     LERYEYTTSVYGHLGQGCIHCRIPFDLYTTPGIQKYRAFMEEAADLVLRYGGSLSGEH
                     GDGQSRAELLPKMFGDELVHAFEEFKRIWDPQWRMNPGKIVDPYRMTENLRLGADYNP
                     PDPATHFHYSADEHSFARAALRCVGVGNCRREEGGTMCPSYMVTREEMHSTRGRARLL
                     WEMLNGDVLTEGWKSEAVKEALDLCLSCKGCKGDCPVKVDMATYKAEFLSHYYEGRIR
                     PRHALAFGFIGLWSQWAGHAPTVANFLTQTPGLRAIAKWAAGMEPKRKIPPFAPQSFQ
                     QWFAARPVRNAGAERVVLWPDTFINYFQPEVGRAAVEVLEHAGFQVEVPREKVCCGRP
                     LFDYGYLNTAKRWLRHVLQTMRPAIESGSPLVVLEPSCAATFRDELVNLFPNNEDAKR
                     LRSQTLLLSEFLEQKAPQYRIPRMQRQALLHGHCHHKAVMGMDAERKVLQKMGLEVNA
                     PDSGCCGMAGAFGYERGDHYDVSIKCGERVLLPEVRRASPEAVIVADGFSCREQILQE
                     TDREALHLAQVIQLAIQEGPPAGERPERRYVQARKEALRKANVRAAAVLGAGILGGVL
                     LYSAIRSGRGRRALAEVFQ"
     gene            7440..7877
                     /locus_tag="PLU99_08665"
     CDS             7440..7877
                     /locus_tag="PLU99_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO17168.1"
                     /translation="MKHELLTEQGGGRTYVLVFATGDEVMQGLKAFAQAEHLSASHFT
                     GIGALQKAMLGYLDCDNKRYLQIPVAEQVELLSIVGNIALGDEGPVIHAHVVLGTRDG
                     QARGGHLVEGHVRPTLELFLTELPGELRKEHDPQTGLNLMRLP"
     gene            7962..10367
                     /locus_tag="PLU99_08670"
     CDS             7962..10367
                     /locus_tag="PLU99_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO17169.1"
                     /translation="MRAAEPVQRRRDSLDLRSPSEHRLMTWVIFLPFLAALAAPLLFR
                     VLGRFTGLALALLPAGLTLYLATRLPWNGAELLTATPWATGLGVDFATRLDGLSATFA
                     LLISGIGVLILIYSAGYFGSDRRQGRFYAFLLSFMGAMLGIVLADDLITLYVFWELTS
                     LFSFLLIGFDHKKPEARSAALKSLLVTTGGGLAMLAGFVLLIIAATQAGKPLAEAARI
                     SALAGLELRGSPFYAPALILIVIGAFTKSAQFPFHFWLPAAMTAPTPVSAYLHSATMV
                     KAGVFLLARLSPALGGTGLWLWLVTPVGAITMLVGAAMAAGQHDVKRILAYATVSVLG
                     ILTMLLGLGTEEAIKAAVVYVFAHALYKVSLFLVAGTLDRQTGTRDVTQLGGLFRVMP
                     AIAMAALLAALSQAGAPPLFGFLGKELLLKVKLDLEWVGRVLIVLATLANIFLVAMAL
                     VIAIWPFYRAPRPTPRQPHHPPLAMSLTALVPAILSLLVGLVPNIFDQTLGLMASAIA
                     GQPVAIKLELWHGLNPAALISMAITAATLTVGFLLFLRLHTWLEHTVVAAHHLERRGP
                     AAAYDFLYEGLERFAKGLTAIVHSGYLRRYVLVILLVLIVAVGYPALRAFPADCGVLQ
                     MSLRWPEAGLALLALAGALLAVAFRSPLASVVALGVTGMAIALLFATFSAPDLAITLV
                     GVEILSVVLFVLLLRHLPREATRRSPRIQLRDALVAVVAGGTMALLVLAAAQVPDDLS
                     IAQFFAKAAYSAGHGRNIVNVILVDFRALDTLGEITVIGAAAMGVLAILRLSRRTQAK
                     ERQ"
     gene            10364..>10706
                     /locus_tag="PLU99_08675"
     CDS             10364..>10706
                     /locus_tag="PLU99_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOO17170.1"
                     /translation="MNSLVLQNATRALVPLLLIYSIFVLFRGHNHPGGGFLGGLLSAS
                     AFVLYGLAYGVRAARRLLRVHPLTLIGVGLLLALASGCAGLLLGRPFLTALWTGVTLP
                     EGSEFGSVLFFD"
BASE COUNT         1826 a         3201 c         3657 g         2022 t
ORIGIN      
        1 aacgcaaaat ctctcttagg gattttgatg ttggcggccg agcgtggggc gcgtgggggc
       61 ggattagggc gcgccctggg cgaggtgctg ctggagagtc aggcgccgca caaagagctc
      121 agcttattcg cgaccgcggc gcgcgtcggc attccggcca cggtgcacgt agcggtgggt
      181 acggatacgg tgcacgtgga tcccggactg gatggggcgg cgctggggcg ggcgacgctg
      241 gcggatttcc acaaggtgtg cgcggtggtc gcggatctgg gggcacgcac ggccggcggc
      301 gcgggcggtg tgtggctgaa cgtggggtcg gcagtcgttc tgcccgaggt cttcctgaag
      361 gcggtgtccg tcgcccgcaa tctcggtcat agtctggaag gcatgcacac cgcgaacttt
      421 gatatgctgc ggcattatcg gccgacacag aacgtcgtgg ggcggccggt ggcgcccggc
      481 ttcgggcaca cggtgatcgg gcagcacgaa attctgttgc cgttactgcg gcaggcggtg
      541 gtcgaagggc tcgcgggcgg agaggtcaca tgaagccgga gaatgcgagc gggctgttca
      601 ttcccgaggc gccggacggc ccggcggcgg ttgaggttga ggagcgtgag ggctgtcgcg
      661 aacgcgacga gcgccgggga agccggacgg aactgctggc gacgttggag cgtttggagc
      721 gaacactgga cgaaatgcgg ggcgggctgg aagcgcgggt gcgcctggag cggcatcgtg
      781 agttttcgcc ggcgcgggcg gtcggggtcg tggtgcaggt gctggtggtg gggctggagc
      841 tgtgggcgct ctctgattgg gtgttcgggg agtacggtca aatactgtat aagctgagct
      901 tcgcgggcgt attgcaagta ggtgcgctga ccgcgttctt gatggggcgg ccggcggagc
      961 gcggttgagg cgcgggttac gggggctgcg ggctacacgg gctgtacggg ttgcggggta
     1021 ctgagtcttt gtagcgcatc ctcgaggctg ggggcctgtt cgaaaaaccg gtccaagcgc
     1081 gtggcggcga acaatccggc gataaagggc ggtgcgccgg ccaggatgac ccgggcctcc
     1141 tggacgttgg cctggccgac catgcggacc aggtcgccca gcgcggcgga attgaggtac
     1201 ttggcgcggg tcaggtcgag cacgatgcgt gcatggggcg tgctcagcat gtcggtgaat
     1261 gtcacaacga gcgagtcgcg gtcttccgtg atgtcgcctt ggatgcgcag caccttgatg
     1321 ccgttcacct cgtccaatct aaggtccact ggcagctcct tgcctgctcg gccttggcgg
     1381 atcgtcgcgg gaaggctgta aagccggcgc ggcgggccgc cggggccccg aaggtatgaa
     1441 tcggcagaac agcggcaaat gcaaaggggt ttagtgaaaa atgcgctgtg caccgtggga
     1501 gtcgcaggta gaagttgccg gcaaaatgtg accaccctgg tgcaacacgg ctacgcgggc
     1561 gtggcggcct tgacgttgga aggccatggc gagcgcgtcc tggcagttat tcgcataacg
     1621 gaacccgagg cgctcggcct cgtccggcct tactccgggc gagaccatga tgcagtcggc
     1681 gtggtcgatg atctgcgcga cgtcggacag aatggcggcg ccgacgaggt cctcgatttc
     1741 gccgcgctgc accatttgca cgatctccgc ttgatggcgg tagccgatct gcatcaggtt
     1801 cgggtgattg tcggccacgc cctcgggatt cggcgcgacg gtgataagcg tgccgccttc
     1861 gcggacggcc atcgtgccgg cgtaggcggc tttggcggac tgccagaaat cgcgatcggc
     1921 cgggtacgcg tcgatcagga caatatcggc gcgctgcggc aaccgcacca taaagacctc
     1981 gcgtgacagg gcgcagccgg cgcggtgcgc gaccacaggg tgaccgacga aacagccgac
     2041 gattttgccg tcggcgtcgg agacgacgtt gacgatgtag cgcagcccgg cgatctgggc
     2101 cgcctcctcc attcgcaggc gcatcgagtt ttccggctcg cccatgaccg tttcggacag
     2161 gcgcatcgag gcttcccact ggttgcgata catcatctcc ttgcccgaga cgcccgggaa
     2221 aatgatcttg gcgccgccac tgaagccttt tacacggtgc ggcacgatgc tgccgatgcc
     2281 caggacgaag tcggcttgcg ccagcaggcg attagcggtg acgcgcgtgc catctttggt
     2341 gcggccgaat tcgtgcatca gcgactcgtc cttccaatca tgctggtgaa tttcgaagcg
     2401 gccgtgcaac tcgcccagct tgtgccgcaa ctcgtcttcg gtcatgcggc gatgcgttcc
     2461 ctgagcggtg aggaattgga cgtggtcgtc cggcgttccg gcggcggcga gctccgccag
     2521 gacgtatggc aggattttcg caacgggcgt gccgcgcgag gcatcatcaa tgatgatcag
     2581 cacgcggtca tgcgggccga cggcgcggtt caggaccgga gcttcataag gcagttgaaa
     2641 gccggcttcg agcacgcgcg gctcgtcaat atccggcagg gcttgcggcc ggtacacgcc
     2701 gatcaggcga tcatcgggga tttcacaagg tgggatgtct ctgtagttgg ggtagttgaa
     2761 ctcgacgcgc acggccggtc tccgttaaca gccggacaga ctagaccttc agccagagct
     2821 gttgcatatc tccctggaaa ggcgtcagaa cttcggggcc tcggtcagtg agtgccacga
     2881 cgtcgcagtg tcttaagccg ccatagccat cgaagtagac cgccggctcg acattgaaga
     2941 tcatgccggt ttgcagcacg tcatctgact tcgggtgcag ccgcgggcgg gcgttcgcgt
     3001 taatggcaga gaagcccacg ccgtgcccgg tcgagtgctt gaacgcatcg ccgaagccgc
     3061 gcgcctgaag gacactacgg gccgccttat cgacatccgc ggcctttacg cctgggcgga
     3121 tggccgccaa tgcggcctgg cgcgcttcgg ccacggcggc gaacatagcg cgttgccgct
     3181 cgtctggctg gccgaggcaa taggtgcgcg taacgtccgt ccaatgcccg tcgacgtatg
     3241 aattgcagtg caccaacacg agatcgcccc gcgccagcgg ccgctgacgc gaacgagcgt
     3301 acgccccgca ggcggccgcg gaattcggcc cggacatgca ccagacgaac ccgccgctgc
     3361 gcgcgatgtc atgcgagtct ccgccgctgt ccagttggtc gcggaacaag gcggccgtac
     3421 gcgattcgct tgtcccgacc gtaacgtgat ccgcgccgta attgaaagcc tcgcccacaa
     3481 tcttgcaggc gagacgcacg cgttcgactt ccgaaggcgt ctgcacggcc gccagttgca
     3541 ccagcaggcc cgaggcgggc gccagctcaa cctggggcgc cacttccttc atcagccgcg
     3601 gcagggcccc gccgtacagg tgcatggccg cataggtaac aggctggtat gactgatcat
     3661 cttcgtaacc catgcgggcg tgcgctaatc ccagcgccgc aagcagcgcg ctcaagggct
     3721 cgcgcagcgc ctgcacggcg ttggtcatac aatctagtga tccggggtga aagacgcgca
     3781 actggtcagc ccagccacgt tccgccaggt cgcgctcgtc ctccggcgcg attacgccca
     3841 cgacgccatc acgcgtgatc accgccacgc tggtgcccac caccggccaa tagccgctaa
     3901 tcagcagcac gtgcattggc agagagcaca cgaggccgtc cagtccctct tgagccaatg
     3961 cggctcgaat tgcctgtaca cgttgttcat cccgcgacat gagtcagctc ccacagtctt
     4021 acaggcccgg cgcagcgggc atctgactat gatagccagg cgcgcggcct gacaactgtt
     4081 gttggcgttc cagtgcgtac agttacacag ttacattgtg gttatccacg gcggacgggc
     4141 agagggattc taagatcggt gagactattg acaataagct cggacacgcc gccgagcgcg
     4201 gcccgagcgg cgatcacata ggagttgtgc catggcgaat ttgacggtgt tgcgccggca
     4261 aatggaaggg cagcggttcg agcgccagct cagtgaagag cgcaccgcgg ccgccgagcg
     4321 cgtcgacatc cgcggcttgg agaatgacct gcggcgcagc gtgcgcggtg aagtccggtt
     4381 cgacaagggt gcacgggcgc tgtacgccac ggacggttcg aactaccgcc aagtgcccat
     4441 cggcgtagtg atcccgcgcg acgtcgagga cgtgatcgcc gcggtggacg tggtgcggca
     4501 atatggcgcg ccgctgctgt cgcgcggcgg tggtacaagc ctggccgggc agtgctgcaa
     4561 tgtcgcggta gtgttcgatt acagcaaata tatgcatcac gtgatcgcga tcgatgccga
     4621 ccgcaaactg gcccgcgttg agccgggctg tgtgctcgac cgcttacgga aggcagcggc
     4681 gccgtacaag ctgacgttcg gccccgatcc ggctacgcat tcgcactgca cgctgggcgg
     4741 catgctgggc aacaattctt gtggcgtaca ctccctgctt agcgccaatg cggggtttgg
     4801 actgcgctct tcggacaaca cgcacgcgct ggacatactg acctacgacg gcgtgcgaat
     4861 gcaggtcgga gaaacgccgc cggctgaact ggaagcggcc attcgcgccg aggggcggcg
     4921 aggcgagatt tacgccgggc tgaagaacct gtccgagaag tatgcggacg agattcggcg
     4981 gcgtttcccg aagctgccgc ggcgtgtttc gggctataac ctggacgagc tgttgcccga
     5041 acgtaatttt cacgttgcgc gggccctggt gggaaccgag agcacctgtg tagtgtgcct
     5101 ggaagcaaca gtgcacctgg tacacaaccc gccggcgaaa tcactgctgg tgttgggtta
     5161 cccggatatt ttctccgccg gcgcgcacgt tatggagatt ctggagcaca agccgaccgg
     5221 cttggagggc gtggaccacc tattgtacca gtggctccaa gaggccggca aggaagaggc
     5281 gaatttgaga ctgttgccgg agggcggcgg ttggctgctg gtggaattcg ggggcgacac
     5341 acgggccgag gcggacgaaa aagcccgcgc gaccatggag atgctcaagg gccagccgca
     5401 gccgccttca atgaagctgt tcgatgaccc tcgggaggag gagatggtct ggaaggtgcg
     5461 cgagagcggg ctgggcgcaa ccgcctgggt gccgggccgc ccggacaact ggccgggctg
     5521 ggaagattcg gccgtgccgc ccgagcaggt cggaccgtat atgcgcgact tcaaggcgct
     5581 gctcgagcga tacgaataca ctacatcagt gtacgggcat ctgggccagg gttgcattca
     5641 ctgccgcatt ccattcgatc tctacactac accgggaatc cagaaatacc gggcattcat
     5701 ggaagaggcg gctgacctgg tgttgcggta cggcggctcg ctatcgggcg agcacggcga
     5761 cgggcagtcg cgggcggaat tgctgccgaa gatgttcggc gacgagcttg ttcacgcctt
     5821 cgaggaattc aaaaggatct gggatccgca gtggcggatg aacccgggca agatcgttga
     5881 cccctatcgc atgacggaga acctgcggct gggcgccgat tacaacccac cggaccccgc
     5941 gacccacttt cattactccg ccgacgagca ttccttcgcc cgggccgcgc tacgctgcgt
     6001 gggcgtgggc aactgccgcc gcgaggaagg cggcaccatg tgcccgagct acatggtcac
     6061 gcgcgaggag atgcactcga ctcgcgggcg ggcgcgcctg ctttgggaga tgctcaacgg
     6121 cgacgtgctg acggagggct ggaagagcga ggcggtgaaa gaggccctgg acctgtgcct
     6181 gtcgtgtaag ggctgcaagg gcgactgccc ggtcaaggta gacatggcga cgtacaaggc
     6241 ggagtttctg tcgcactatt acgaaggccg cattcgcccg cggcacgcac tggcttttgg
     6301 cttcatcggc ctgtggtcgc aatgggccgg ccacgcgccg accgtggcga acttcttgac
     6361 gcaaacgccg ggcttgcggg cgatcgccaa gtgggcggcg ggcatggagc cgaaacgcaa
     6421 gattccgccg ttcgcgccac agtcattcca gcagtggttc gcggcgcgcc cggtgcgaaa
     6481 cgccggcgcc gagcgcgtcg tcctctggcc ggatacgttc atcaactact ttcagcccga
     6541 ggtcggccgg gccgccgttg aggtgctgga acacgccgga ttccaggtag aggtgccgcg
     6601 ggagaaggtg tgttgcgggc gaccgctgtt cgactacggc tacctgaata cggccaaacg
     6661 ttggctgcgg cacgttttgc agaccatgcg gccggcgatc gagtccggca gcccactggt
     6721 agtactggaa ccaagctgcg cggcgacgtt ccgcgatgag ctggtcaatc tctttcccaa
     6781 caacgaagac gccaagcgcc tcaggtcaca aacgctgttg ttgagcgagt tcctcgagca
     6841 gaaggcgccg cagtaccgca taccgcggat gcagcgacag gccttgctgc acggacactg
     6901 tcatcacaaa gccgtcatgg gcatggacgc cgaacgcaaa gtcctccaga aaatgggcct
     6961 ggaggtcaac gctccggaca gcggctgttg cggcatggcc ggcgcgttcg gctacgagcg
     7021 cggcgaccac tacgacgtct cgatcaagtg cggcgagcgc gtgctgctac ccgaagttcg
     7081 tcgggcctcg ccggaggcgg tcattgtcgc cgatggtttc agttgccgcg agcaaatatt
     7141 gcaggaaacg gaccgggagg ccctgcacct ggcacaggta atccaattgg cgatacagga
     7201 gggcccaccg gcaggcgagc ggccggagcg ccgctacgtt caggcgcgta aggaggccct
     7261 gcgcaaggcg aacgtgcggg ccgcggcggt actcggggcc ggcattttgg gcggcgtcct
     7321 gctgtactct gctatccgtt ccggccgcgg ccgtcgagcg ttggccgagg ttttccagta
     7381 gaattctaag ccgccaactg aggcggcatg gtgtgccgga agatggcagg tggtggcgca
     7441 tgaaacatga attgctgacc gagcaaggcg gggggcgcac gtacgtactg gtgttcgcga
     7501 ccggcgatga ggtaatgcag gggctgaaag cgttcgcgca ggccgagcac ctgagcgcca
     7561 gccacttcac cggcatcggc gcgcttcaaa aggcgatgct ggggtatttg gattgcgaca
     7621 ataagcgtta cctgcaaatt ccggttgcgg agcaggtgga gctgctctct atcgtcggga
     7681 acatcgctct tggagacgag ggcccggtga ttcacgcgca cgtcgtattg gggacgcgcg
     7741 acggccaggc ccgcggcggc cacttggtag agggtcacgt gcgcccaact ctggaattgt
     7801 tcctgacaga gttgccggga gaactgcgga aagaacacga cccgcagacc gggctgaatc
     7861 tgatgcgcct gccttgaagc tgcctcgcgg cggcgtgccc ctttgcgttg cgctcgcgga
     7921 tagcagtcgc gtaacatact attctctctc tagcagcgtg attgcgcgcc gctgagccgg
     7981 ttcagcggcg gcgcgactcg ctcgatctcc gtagtccgtc cgagcaccgc ctgatgactt
     8041 gggtgatttt ccttccattc ctggcagcgc ttgcggcgcc gctgctcttt cgcgtgctcg
     8101 gccgctttac gggcctggcg ctcgcactat tacccgccgg cttgacgctc tatctggcca
     8161 cacgcctgcc ctggaacggc gcagagctgc tgacggccac accgtgggcg actgggctgg
     8221 gggttgactt cgcgacacgc ctggacggcc tgtcggcgac attcgcacta ctaatttccg
     8281 gtattggcgt cctgattctg atctattcgg cggggtactt cggtagcgat cgccgacaag
     8341 gccggttcta cgcgttcctg ctgtcgttca tgggggcaat gctgggaatc gtactcgcgg
     8401 atgatctgat cacgctctac gtgttttggg aactgaccag cctgttttcg ttcctgctca
     8461 tcggattcga tcataaaaag ccggaggcgc gcagcgcggc tctgaaatct cttctggtaa
     8521 cgactggcgg cgggctggcg atgttggcgg gattcgtgct cctgataatc gcggccacgc
     8581 aggcgggcaa accgctggcc gaggccgcgc gaatatccgc cctggcaggt ctcgaactgc
     8641 gcggcagccc tttttacgcg cctgccctaa tcctgattgt gatcggagca ttcacgaaat
     8701 ccgcacaatt tccgtttcat ttctggctgc cggcggccat gaccgcaccg acgccggtca
     8761 gcgcatacct gcactcagca accatggtca aggcgggcgt ctttttgctg gcccgcctgt
     8821 ctcctgcgct gggcggaacc ggcctgtggc tctggctcgt cacgccggtc ggcgcgatca
     8881 ccatgctcgt tggagcagcc atggcggcag gtcagcacga cgtgaaacgc atccttgcct
     8941 acgcaaccgt cagcgtgctg ggcattctca ctatgctgct tggcctggga accgaggagg
     9001 cgatcaaggc cgccgtggtg tacgtcttcg cacatgcact ctacaaggta tccctgtttc
     9061 tcgtcgccgg caccctggac cgccaaaccg gcacgcgcga cgttacgcaa cttgggggct
     9121 tgttcagggt gatgcccgcg attgccatgg ccgcgctgct tgcggccttg agccaggccg
     9181 gcgcgccgcc actgttcgga ttcctgggaa aggaactgtt gctgaaggtc aagctcgatc
     9241 tggaatgggt cggccgagtg ctgatcgtct tggccacctt ggccaacata tttcttgtcg
     9301 cgatggcgct ggtaattgcc atttggccgt tctaccgcgc gccgcgtcca acgccgcgcc
     9361 agccgcatca tcccccgctg gccatgagcc tgaccgcgct ggtgccggcc atcctaagtc
     9421 tgttggtcgg cctggttcca aacatttttg accagacact cgggctgatg gcttcggcca
     9481 ttgccggcca gccagtggcc ataaaactgg aactgtggca cgggttgaac ccggcggcgc
     9541 tgatcagcat ggccatcacg gcggcgacgt tgacagtggg gttcctgttg ttcctgcgct
     9601 tacatacctg gctggagcat acggtggtgg ccgcgcacca cctggagcgg cgcggaccgg
     9661 cggcggcgta cgacttcctg tatgaggggt tggaacgctt cgcgaagggc ctcactgcaa
     9721 tcgttcacag tgggtacttg agaagatacg tgctcgtgat tctgttggtg ctgatcgtgg
     9781 cagtgggtta cccggcgctg cgcgcttttc cggcggattg tggagtgctg caaatgtcgc
     9841 tgcgctggcc cgaggcgggc ttggcgttgc tggcgctggc cggtgcgctg ctggccgtgg
     9901 cttttcgctc gccactggct tcggtggtcg cgctcggtgt gaccggaatg gcaattgcgt
     9961 tgttgttcgc cacgtttagc gcgcccgatc tggcgataac gctggtaggc gtcgaaatcc
    10021 tcagtgtagt gctgttcgtg ctgctcttga ggcatttgcc gcgggaggcc acgcgccgca
    10081 gtcctcggat ccaactgcgc gatgcgctag tagccgttgt cgcgggcggc accatggcat
    10141 tactggtgct ggcggctgcc caggtgcccg acgacctctc catcgcccag ttctttgcca
    10201 aagccgccta ctccgccggc cacgggcgca acatcgtgaa cgtgatcctg gtggacttcc
    10261 gggccctgga cacactgggc gaaatcactg tgatcggcgc cgcggccatg ggcgtgcttg
    10321 ccattctgcg cctgtctcgg cggacccaag cgaaggaacg gcaatgaact cactcgtact
    10381 gcaaaacgcc acgcgggccc tggtccccct gctcttgatc tactcgatct tcgtgctttt
    10441 tcggggccac aatcaccccg gcggcggctt cctcggcggg ttgctcagcg cctcggcgtt
    10501 tgtgctgtac gggctggcgt acggtgtgcg cgcggcgcgc cgccttctgc gcgtgcaccc
    10561 gctcacgctg atcggcgtgg ggctgctgct ggccttggcg agcggttgtg ccggtctgtt
    10621 gctcggccgg ccctttttga cggccctgtg gaccggtgtc accctgccgg agggcagcga
    10681 gtttggatcg gttctgttct tcgacc
//
LOCUS       DAOSYS010000118        10702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5267_length_10702_cov_5.479008, whole genome shotgun sequence.
ACCESSION   DAOSYS010000118 DAOSYS010000000
VERSION     DAOSYS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_10702_cov_5.479008"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /gene="folP"
                     /locus_tag="PLU99_08680"
     CDS             complement(<1..457)
                     /gene="folP"
                     /locus_tag="PLU99_08680"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOO17171.1"
                     /translation="METDVAADNVQILGILNVTSDSFSDGGRFLDTADAVAHGRQLIE
                     DGADIIDIGAQSTNPDAEYITAQQELERLTPVVQQLKAEGIRISVDTFRPQVMRAMVA
                     AGAEFVNDVTALRDPEAVAAVRDCGARVILMHSTATAARAERADISAAGM"
     gene            754..1146
                     /gene="rpsL"
                     /locus_tag="PLU99_08685"
     CDS             754..1146
                     /gene="rpsL"
                     /locus_tag="PLU99_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOO17172.1"
                     /translation="MPTMNQLVRHPRRTAPKKSKVRDLEACPQRRGVCLQVKTQTPKK
                     PNSALRKVARVRLTNGREVTAYIPGVDHNLQEHSIVLVRGGRVRDLPGVRYHIIRGTL
                     DAAGVGNDKEGKGRNRGRSKYGVKRRKK"
     gene            1251..1736
                     /gene="rpsG"
                     /locus_tag="PLU99_08690"
     CDS             1251..1736
                     /gene="rpsG"
                     /locus_tag="PLU99_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOO17173.1"
                     /translation="MAYKRWTVSEKQLTGDPRFNSRLAAKFINCMMWDGRKSVSMRVF
                     YDAMDIVAERVKDVPPIEVFEKAIENIKPNIEVRSRRVGGANYQVPMQVEAKRRQSLA
                     IRWVLAATRGKTGKPMADRLASELLAAYNGEGDAMKKREEVHRMADANKAFAHFAWSR
                     R"
     gene            complement(1848..3419)
                     /locus_tag="PLU99_08695"
     CDS             complement(1848..3419)
                     /locus_tag="PLU99_08695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO17174.1"
                     /translation="MKHLLPPLIVLALVPLVLAQGQSAPPQAPPQPAAQASAGAPAAN
                     PGLHPRVKLETSLGDIIIELDGEKAPISTQNFIRYAEEGFYNGTIFHRVIKTFMIQGG
                     GWTPELTKKTQGIHPPIKNEWQNGLKNVRGAIAMARTDEPDSATAEFFINVVDNPNLD
                     TPRQPRRGGPPAAYAAFGRVVEGMDVVDKIKEVPVQKSSKMPSSDAMPTVPIEPVIIK
                     SVTLVSEYDRARVEQAAAEAVQKADEERAKAEQEREKALQDLTAKIEGEIGKKMEKTP
                     RGLMYAITAEGQGASPKLGDTVSILFTGSLPDGKVWDSATDRSKPRTMALNPRETLPG
                     LIEGLSLMKPGGKAKFIVPPQLAYGPGARLDKTIPPDSTLIFDVELLEVKTAEQMAAA
                     SAQEEQEFIAKIEAETGKKFEKTASGLMYLKLTEGNGPAVTPTSKVRAHYTGWLLNGT
                     KFDSSVDRGQPIPVDLSRGGVIPGWIEGLKLMKLGDKWKFVIPPELAYGRAGRPPVIP
                     PNSALVFDIEVVGVE"
     gene            3782..5689
                     /locus_tag="PLU99_08700"
                     /pseudo
     CDS             3782..5689
                     /locus_tag="PLU99_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
     gene            6016..6810
                     /locus_tag="PLU99_08705"
     CDS             6016..6810
                     /locus_tag="PLU99_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO17175.1"
                     /translation="MELPTEHIRLDCAALHFARDVYRDFEFDRYLRQLDALAAEVGDR
                     RPGLSATLRYQAMREVLVEEHGFRGDVKNYSDPQNSYLNRVLDRRLGSPVSLSVIWIE
                     VARRLKWPVHGVALPGHFLVRFDDPEHLVLADPFNGGRSLSVGDCRRLVNNLTNCPVP
                     FSTRMLAPVDTRATLARMLHNLRRIYLALGDWQRLAAVLRRLVALEPTAGQHLQDLAS
                     VCARRGDVRGAYGCLHLYLRRVPDARDCDLVQSNLSRLEAAMLARN"
     gene            complement(6841..8043)
                     /gene="amrS"
                     /locus_tag="PLU99_08710"
     CDS             complement(6841..8043)
                     /gene="amrS"
                     /locus_tag="PLU99_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOO17176.1"
                     /translation="MDDLRNIFTCPSRRDLLSWGVAATGCLLAGRRLLAQDQASGTSA
                     LPGPKLKGLARHEALYWEPRPEGRVQCTLCPRACEVADVERGYCGVRENQGGKYQTLV
                     YGALCSANVDPIEKKPLFHYLPGTQAFSIAAAGCNMECKFCQNWQISQFRPEQVESVL
                     VDPERLVRTCQAQKCPTIAYTYSEPIVAYEYMHDSAALARAAGVGSVMISNGYIQRPA
                     LVQLCRQLTGVKIDFKAFTESFYRDWCAAELKPVLATLSALKEIGIWFELVMLVIPTL
                     NDSPEEIRDMSRWIVKELGPDVPVHFTRFHPTYRVTNLPRTPVATLDACRRIALDAGI
                     HYVYAGNVPTHPGENTYCHSCQTELIRRVGYQIKSNEIKDGKCPKCGVTIPGVWSQEQ
                     ALAFKPRV"
     gene            complement(8045..9055)
                     /locus_tag="PLU99_08715"
     CDS             complement(8045..9055)
                     /locus_tag="PLU99_08715"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO17177.1"
                     /translation="MSGSSTRREFLTRAGAMAAGAALGTCGRLALVGASGLDQSEPGR
                     ARVVIARDEALATGEPGEHGALLLKVLNAAMQKLTGAADAASAWRTVFKPADRVGIKV
                     NTLGLSTQPAVVDAIIAGLRLADVPAANIIVWDRFDTELGRAGFTISKSGTGVRCRGT
                     DAEGYGSGYRPEIEQSGAVGSAFSRIVADEVDALISVPVLKDHNLAGVSLGMKNFYGA
                     IHNPNKYHEHNCDPYIVDVVAHRYIRPKWRLTVCDGLRAQCHAGPGHHPGYMWPFGGL
                     IVGTDFVATDAVATSIIEEQRKQRGLKTLAEEGRPARHVATAAARGLGIADMSRIERI
                     EI"
     gene            complement(9095..10591)
                     /gene="amrB"
                     /locus_tag="PLU99_08720"
     CDS             complement(9095..10591)
                     /gene="amrB"
                     /locus_tag="PLU99_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOO17178.1"
                     /translation="MRMPRSILPVYLPICVLALLVATACADATVRPAACAGQWYPADA
                     ATLTKQIDELLSAATPLDLCGRPLAVISPHAGYRFSAPVACAGYACLRGQSYQRVIVL
                     AFSHRNAGRYRGIDVPKELTAYATPLGEVPIDRAVCDELLKKPFFSSIPDIDEGEHSL
                     ELQLPFLQRILGKFALVPLLVGQMTDADCTAAAQALLPYLDENTLLVASSDFTHFGPN
                     YGYLPFKENVPDKLRELADAAAAPLRLCDYDGFAEHVARTHDTICGRGPIMLLLRTLA
                     MRGGADGVCAACDTSGRMMGDWTNSVTYQSLVFTPRKGTLGEAERAELLRLARQTVAA
                     TVKKEPLPKVAPEKLPAAVRADGSCFVTLENNGELRGCIGNMLADGPLFESVVRNAVH
                     ACHDSRFLHNPVTPEELEQLHVEISYLTPMKRAASPSEVVVGRHGIMIALGGRRGVLL
                     PQVAYERGWTREEFLKQVCYKAGLPVDSWNKPEAELYLFEAEVFGEPK"
BASE COUNT         1968 a         3500 c         3283 g         1951 t
ORIGIN      
        1 ccatgcccgc cgcactgatg tccgcacgct ccgcccgagc cgcggtggcc gtggagtgca
       61 tcaggatgac ccgcgccccg cagtcccgaa cggccgccac ggcctcggga tcacgcaggg
      121 cggtcacgtc gttaacgaat tcggcgccgg ccgccaccat ggcccgcatg acctgcggcc
      181 gaaaggtatc caccgaaatg cggatcccct cggccttgag ctgctgcacg acgggtgtca
      241 gccgctccag ttcctgctgc gccgtgatgt actccgcatc cggattcgtg gattgtgcgc
      301 cgatgtcgat gatgtccgcc ccatcctcga taagttgccg cccgtgtgcc acggcgtccg
      361 cggtgtccag aaagcgcccg ccatccgaaa aggaatcgct cgtaacgttc agtatgccca
      421 ggatttgcac gttgtccgcc gccacgtcag tttccatcgg cgccgattct aacccaaggc
      481 caaggcggcg tcccacgcac ccgctaccgc aaacctgccc tccctgcccc cgagccgccc
      541 ccgggccagg ccgccggacc ggggccgcgg cgccaatagt tggcccgcga ccggcccccg
      601 ggggtaccga ccggccgccg gaccgcgcga agcccaaacc acacatggcg tcccaatttt
      661 gccgtccgag ccttggcagg cccatcggtt ccggtatact acgggactcg ccgggttcgc
      721 gccggcatac aggactcaag gagcaggctt aatatgccta cgatgaacca gttggtacgg
      781 catccgcgcc gcaccgcgcc gaagaaatcc aaggttcgcg atctcgaggc ttgcccgcaa
      841 cgccgcggcg tctgcctgca ggtcaagacc cagacgccca agaagccgaa ttccgccctg
      901 cgaaaggtgg cccgcgtgcg cctgaccaac gggcgcgaag tgaccgccta tattccgggc
      961 gtcgatcata acctgcagga gcactcgatc gtgctggtgc gcggcggccg tgtccgcgac
     1021 ctgcccggcg tgcgctacca cataattcgc ggcacgctgg atgcggccgg cgtcggcaac
     1081 gacaaagaag gcaagggccg caaccgcgga cgcagcaagt acggcgttaa gcgccgcaag
     1141 aagtaacgca gggtccgtcg ctccagtcag cggcgacgcg tgccactgct cgcatcagcg
     1201 gtgaacgaga gaacacgaaa gcaccgagat tggctcacag cgggtgaaga atggcttaca
     1261 agcgttggac ggtttcagaa aagcaactga ccggcgaccc ccggttcaac agccgcctgg
     1321 cggcgaagtt cattaactgc atgatgtggg acgggcggaa gtccgtctcg atgcgcgtct
     1381 tttacgacgc catggacatc gtcgccgagc gcgtcaagga cgtgccgccg atcgaagtat
     1441 tcgaaaaggc gattgagaac atcaagccga acatcgaagt ccgctcgcgg cgtgtaggcg
     1501 gcgccaacta ccaggtgccg atgcaggtcg aagccaagcg ccgccagtct ctggcgattc
     1561 gctgggtcct ggccgccacc cgcggcaaga ctggcaagcc catggccgat cgcctggcca
     1621 gcgagctgct cgccgcctac aacggcgagg gtgacgccat gaagaagcgt gaggaagtgc
     1681 accgcatggc tgacgcgaac aaggccttcg cgcactttgc ctggtcccgc cgctagaagc
     1741 cgaactgaca cccgaaaata taaacggccg ggctaatcca gcccggccgc ttccgttttg
     1801 cgacgcgagg gcccgcaagc ggcaccctcc ctatccaacg tttcgcgcta ctcgaccccc
     1861 acgacctcaa tatcaaacac cagcgcggaa ttcggcggga tgaccggcgg gcggcccgca
     1921 cggccatacg cgagctccgg cggaatgacg aacttccact tgtcgcccag cttcatgagc
     1981 ttcagcccct caatccagcc gggaatgacg ccgccgcggc tcagatcgac tggaatcggc
     2041 tgcccgcggt cgaccgagct gtcaaacttg gtcccgttca gcagccagcc ggtgtagtga
     2101 gcccgcacct tgctggtggg cgtgacggcc ggtccgttgc cctcggtcag cttcaggtac
     2161 atcaggccgg aggcggtttt ctcgaacttc ttgccggttt cggcttcgat cttggcgatg
     2221 aactcctgct cttcctgtgc gctggcggcc gccatctgtt ccgcggtctt cacttccagc
     2281 agttccacgt cgaagatcag ggtcgaatcg ggcgggatgg tcttgtcgag gcgcgcgccc
     2341 gggccatagg ccaactgggg cggcacgatg aatttggctt ttccacccgg cttcatcagg
     2401 ctgaggccct cgatcagacc ggggagcgtt tcgcgcgggt tcagggccat ggtacgcggc
     2461 ttacttcgat cggtcgcgga atcccagact ttgccgtccg gcagcgaacc ggtgaagagt
     2521 attgaaaccg tatcgccgag cttgggcgag gcgccttggc cctcggcggt gatcgcatac
     2581 atcagaccgc gcggcgtttt ttccattttc ttgccgattt cgccttcgat cttggcggtc
     2641 agatcctgca gcgccttttc cctctcctgc tccgccttgg cccgttcctc atcggccttc
     2701 tgcaccgctt cagcggcggc ctgttccacg cgggcgcggt catattcgct gacgagcgtg
     2761 accgacttga tgatcaccgg ctcgatcggc acagtcggca tggcatcgga tgagggcatc
     2821 ttggagcttt tctggaccgg cacctccttg attttgtcga ccacgtccat gccctcgacc
     2881 acgcgcccga aggcggcgta ggccgcgggg gggccgcccc tgcgtggctg gcgcggcgta
     2941 tccaggttcg ggttgtccac gacgttgatg aagaactcag cggtggcgct gtccggctcg
     3001 tcggtacggg ccatagcgat cgcgccgcgc acgttcttca gaccgttttg ccactcattt
     3061 ttgatgggcg ggtgaatccc ctgggtcttc ttggtcagtt ccggcgtcca gccgccgccc
     3121 tggatcatga aggtcttgat gacgcggtgg aagatggtgc cgttgtagaa gccctcttcg
     3181 gcgtaacgga tgaagttctg cgtggaaatg ggcgcttttt cgccatccaa ctcgatgatg
     3241 atgtcgccca aggaggtttc aagcttgacg cgcgggtgca agccggggtt cgccgccggg
     3301 gcgccggccg acgcctgagc cgccggctgc ggtggagctt gtggcggagc gctctgccct
     3361 tgagccaaca cgagcggtac tagcgcgaga acaatcaacg gcggtagaag gtgcttcatg
     3421 gcgttttgtc ctttcatcaa cgaaccaacc cacggggcgt ttagtccgtt aacgccacgg
     3481 ccccgcggca aaacgtagta gcatatcgcc aagggcggga gggcgatacg gccgaaatag
     3541 tgccaacggc agcgccgcgg gtgggctatg ccaaaggggc ggaccaagaa gcgcgccgtt
     3601 cgagttgcca gtgcttggaa aacgtcaatc ggcgaacccg ccgacagcgg aggcccacgt
     3661 tgcttagccg cctgccaccc cttatgttgc ttggaccctg gccaggccgg agcgttccgg
     3721 cgagccgggg tcatctggct gctagtgccc gcgcggtcgc ccgactgccg aggaggagaa
     3781 tcgcatgagt gacgcggatt ttgaggcact ctatcgcagg tcaatcgagg atccggaggg
     3841 cttctgggcc gggccggccg aggacattca ctgggtccgc aagtgggacc gcgtgctcga
     3901 cgactcgcgc aagccttttt accgctggtt cgtcggcggc gagatgaaca cctgttacaa
     3961 cgcgattgac ctgcacgtgg agcagggccg aggcgagcag gccgcgatca tctacgacag
     4021 ccccgttacg aacagcgtgc gaacgataac ttttcgggag ctgcgcgacc tggtggcgcg
     4081 cttcgccggg gccctgaccg cccagggcgt cacgaagggg gaccgcgtca tcatttacat
     4141 gccgatgatc cctgaggcgc tcgtcgccgt atacgcctgt gcgcgcatcg gcgccatcca
     4201 ctccgtcgta ttcggcggct tcgcgccgaa agaattggcc aagcgcatca ctgacgccca
     4261 gcccaaactg atcgtttccg cctcctgcgg aatcgagggc aagaaaatca tcgaatacaa
     4321 gccgctgctc gacgaggcga tcggcatgtc cagtgctaag ccgcaacgct gcatcatctt
     4381 ccagcgcccg caggcgcagg ctgagctgca cgcgggacgc gacctggatt ggaatgacgt
     4441 gatggcggcc gccaagccgg tcgactgcgt tccgctcaag gcgaccgacc cgctttacat
     4501 tctctacacg tccggcacca ccggcgttcc gaagggcgtc gtgcgcgaca acggcgggca
     4561 cgccgtcgcc atgaaatgga ccatgaaagc catttacaac gtcaaccccg gcgacgtcta
     4621 ttgggccgcc tcggacatcg gctgggtcgt cgggcattcg tacatcatct acgggccgct
     4681 gctgaagggc tgtacgacag tactctacga aggcaagccg gtcgggacgc cggacccggg
     4741 cgccttctgg cgcgtcatct ctcagcacaa ggtaaacgtc ctgttcaccg ctccgactgc
     4801 gttccgcgcc atcaagcgcg aggacccgaa ttgcgagtat ctcaagcgtt acaacctggg
     4861 cgacttccgc gccctgttcc tggccggcga acgctgcgac ccggacacgt tgcgctgggc
     4921 ccaagagcac ctcaaagtgc ccgtcatcga ccactggtgg cagaccgaaa ccgcctgggc
     4981 cattgccgct aactgcttgg gtctgtgcaa cttaccggtt aaaccgggct caccaaccaa
     5041 ggcgtcgccg ggctacgacc tccgcgtgct ggacgaaagc ggcgatcccg taccgcaggg
     5101 caagatcgga aacctggtga tcaagctgcc gctgccgccc ggctgtctga ccacgctatg
     5161 gaataatgac cagggcttta aggaatcgta cttatcggcc tatcctgggt actacctcac
     5221 cggcgacgcc ggctatatcg atgacgacgg ctatgtctac atcatggccc gcatcgatga
     5281 catcataaac gtcgccgggc accgcctttc cacgggcgga atggaggaag tgctcgcgga
     5341 gcacccggcc gttgcggaat gcgcagttat cggtgttaat gatcaactaa aaggcgaggt
     5401 gccgctgggg ttcgtcgtcc tcaaatccgg cgtggaccgg ccgaaagacg agctgactca
     5461 agaactgttg cagatggtgc gcgctcgcat tggcgcggtg gcgtcattca agctggtgac
     5521 tgtcgtgcag cgcttgccca agacgcgctc cggcaaggtg ctgcgcggca cgatgaagaa
     5581 gatcgccgac gggagcgagt accaaatgcc tgcgaccatt gacgatcccg ccattttggg
     5641 tgaaatcaag gaggcgttgg ccacactcgg atacccgaag cgcgcttaac gcgaattcag
     5701 ttgtcagcaa actgcgaatt gcggcaagca tttctgttga caacgacaga aaaaaaaatt
     5761 tgccaattct tgcagattgt ggctttcgtc ccgctcgcgg ccggaaccgc gcccattccg
     5821 cctagagggc cgatgttatc gcaaccttac acatttatgg gagcagccgt gcgagtctga
     5881 acacgcgcgg cgcaaaaatt ccctcgcatt tacgggggtt gggatgatat gatgcgcaga
     5941 gtcggctcag acgaggaatc gaacagacta ctaacagggg catataaggt gggtcaaagt
     6001 tcatttgact tgctgatgga actcccgacg gagcacatcc gccttgattg tgctgcgctt
     6061 cacttcgcgc gtgacgtata cagggatttc gagttcgacc gctacctccg acagctcgat
     6121 gctctggcgg ccgaagtcgg cgaccggcga ccgggattgt cggccaccct acgctaccag
     6181 gccatgcgcg aggtgctggt cgaggagcac ggctttcgcg gcgacgtaaa aaactattcc
     6241 gatccacaga acagttatct caatcgcgtg ctggaccgcc gattgggtag tccggtctcg
     6301 ttatccgtga tttggatcga agtagcccgg cgtttgaaat ggccggtgca cggagtcgca
     6361 ttacccggcc acttcctggt gcgatttgac gaccccgagc atctggtttt ggcagacccg
     6421 ttcaacggcg ggcgctcgct ctcggttggc gactgccggc gcctcgtaaa caacctcacg
     6481 aattgcccgg tgcccttctc gacgcgcatg ctggctccgg tcgatacccg ggccacgctg
     6541 gccagaatgc tgcacaacct gcgccgaatt tacctggcgc tcggcgattg gcagcgcctg
     6601 gccgcagtgc tgcggcggct cgtggcgctt gagccgactg ccggccagca tcttcaggat
     6661 ctggcgtcgg tgtgcgcccg ccgcggcgac gtgcgcggcg catacggctg tctgcacctg
     6721 tacctgcggc gggtgcccga cgcacgcgac tgtgacctgg tgcaatccaa cctgtcgcgt
     6781 ctcgaagcgg caatgctggc gcggaattaa cccggcctgg cgaaaatacg tcacagatcg
     6841 ctatacccgt ggcttaaagg ccaacgcctg ctcctgtgac caaacgcccg gtattgtgac
     6901 gccgcacttc gggcacttgc cgtccttgat ttcattggac ttgatctgat agccaacgcg
     6961 ccggatcagc tcggtttggc agctatggca ataggtgttc tcgccggggt gcgtcggaac
     7021 attaccggca tacacgtagt gtatgcccgc atccaatgca atgcggcggc aggcgtccag
     7081 cgtcgcaacc ggcgtcctgg gcaggttcgt gacgcggtag gtcgggtgga aacgcgtgaa
     7141 atgcaccggc acgtccggcc ccagttcctt tacgatccac cggctcatgt cccgaatttc
     7201 ctcgggtgag tcgttcagcg tcggaatcac cagcatcacc agttcaaacc agatgccgat
     7261 ttccttcagc gcgctcagcg tcgccagaac cggcttcagc tcggccgcac accagtcgcg
     7321 gtaaaagctc tccgtaaagg ctttgaagtc gatcttcacg ccggtcagtt ggcggcataa
     7381 ctggaccagc gccggccgct gtatgtagcc gtttgaaatc atcacgctgc cgaccccggc
     7441 ggcgcgggcc aatgcggccg agtcgtgcat gtactcgtaa gcgacgattg gctcggaata
     7501 agtgtaggcg atcgtcggac atttctgcgc ctggcacgtg cggaccagcc gttcaggatc
     7561 gacgagcacg ctttccacct gttccggccg gaactggctg atttgccagt tctggcagaa
     7621 tttgcattcc atgttgcagc cggccgcggc gattgagaac gcctgtgtgc cgggcaggta
     7681 atggaaaagc ggcttctttt cgattgggtc cacgttggcc gaacacaacg ccccatagac
     7741 cagagtctgg tacttgccgc cctgattttc gcgcacgcca cagtagccgc gttccacgtc
     7801 ggccacttcg caggcgcgcg ggcagagggt gcattggacc ctgccttcgg ggcgcggttc
     7861 ccagtacagc gcttcatgcc gcgccagacc cttgagtttt ggaccgggca gcgcgctcgt
     7921 gcctgaggcc tgatcctgcg ccagcaatcg ccggcctgcc agcaggcacc cggtggccgc
     7981 gacgccccag ctcagcaggt cacggcgcga tggacaggtg aatatgttcc ggagatcgtc
     8041 catgtcagat ttcaatccgc tcgatccggc tcatatccgc aattcccagc ccgcgcgcgg
     8101 ccgcggtggc gacgtgccgc gccggccgcc cctcctccgc caacgttttc aatcccctct
     8161 gctttcgctg ctcttcgata atagaggtag cgacggcatc ggtcgccacg aagtccgtcc
     8221 ccacgatcag cccgccgaac ggccacatgt agcccgggtg atggccgggc ccggcgtggc
     8281 attgggcgcg caggccatcg cagaccgtca gccgccattt gggccggata tagcgatgag
     8341 caaccacatc cacaatgtat ggatcgcagt tgtgctcgtg atacttgttg ggattgtgga
     8401 tggcgccgta aaagttcttc attcccaacg acacccccgc cagattgtgg tctttaagca
     8461 ccgggaccga tatcagcgca tcgacctcgt ccgcgacgat tctcgaaaac gccgacccga
     8521 cggcgccact ctgctcgatt tccggccggt atccactgcc gtagccctcg gcgtctgtgc
     8581 cgcggcagcg cacgcctgtg ccggatttgc tgatcgtaaa gccggcccgg cccagctccg
     8641 tatcgaaccg atcccaaaca atgatgttcg cggccggcac gtccgccagt cgcaggccgg
     8701 caataatggc gtctacgacc gccggctgcg tcgagagccc gagcgtattg accttgatgc
     8761 ccacacggtc ggccggtttg aacactgtgc gccaggcact ggccgcatcc gcggcaccgg
     8821 tcagcttttg catggccgcg ttaagcactt tgaggagcag cgcgccatgt tcgccgggct
     8881 cgcccgtggc caatgcctcg tcgcgcgcga ttacaacccg cgcccggccc ggctcgctct
     8941 ggtccaagcc agaagcgccg acgagggcca ggcgcccgca cgtccccagc gccgcccccg
     9001 ctgccatcgc tccggcacgc gttagaaact cgcgccgcgt actgctgcca gacatcatcc
     9061 gccttacctt cgctccggcc cgcccagcgc actcctactt cggctcgcca aacacttccg
     9121 cctcgaacag gtaaagctcc gcctcgggct tattccagga atcgaccggc aggcccgctt
     9181 tgtagcatac ctgcttgagg aactcctcgc gcgtccagcc gcgctcgtac gccacctgcg
     9241 gcaacagtac gcccctgcgc ccgccgagcg cgatcatgat tccgtgccgc ccgacgacga
     9301 cctcgctggg actggccgcc cgcttcatcg gcgtgagata gctgatctca acgtgaagtt
     9361 gctccagttc ctccggcgta accgggttgt gcaggaagcg cgagtcgtgg caggcgtgca
     9421 ccgcgttgcg cacgaccgat tcaaacagcg gcccatccgc gagcatattg ccgatgcacc
     9481 cccgcagctc gccgttgttt tcaagcgtga cgaagcagga cccgtccgca cgcacggccg
     9541 ccggcagctt ctcgggcgcg accttcggca gcggttcctt tttcaccgtc gcggctaccg
     9601 tctgccgtgc cagccgcagc agttccgcgc gttcggcctc gcccaaagtg cccttacgcg
     9661 gagtgaacac cagcgactgg tacgtgaccg agttggtcca atcccccatc atacggcccg
     9721 acgtatcaca cgccgcgcag acgccgtcgg cgccaccacg catcgccagc gtacgcagca
     9781 gcagcataat tggcccgcgc ccgcaaatgg tgtcatgcgt cctggccaca tgctcggcaa
     9841 agccgtcgta atcgcacagc cgcaacggcg cagccgcggc gtccgccaat tcgcgcagct
     9901 tgtccggcac gttctccttg aacggcaggt agccgtaatt ggggccgaaa tgcgtgaaat
     9961 cgctgctggc gaccagtagc gtgttctcgt ccaggtacgg caacagcgcc tgggccgcgg
    10021 cagtgcaatc agcgtcggtc atctgcccga ccagtagcgg aaccagcgcg aattttccga
    10081 ggatgcgctg caagaacggt aattgcagct cgagggaatg ctcgccttcg tcgatgtcgg
    10141 gaatcgagct gaagaagggc tttttcagca gctcgtcgca aacggcgcga tcgatcggca
    10201 cttcgcccag cggcgtggcg taggccgtca gctccttggg cacgtcgatg ccgcgatagc
    10261 gtccggcatt gcggtgactg aaggcaagca cgatcacgcg ttgataggat tgcccgcgca
    10321 ggcacgcgta gccggcgcac gccaccgggg ctgagaagcg gtaccccgca tgcggactga
    10381 tcaccgccag cggccggccg cacaggtcca gcggcgtggc agccgacagc agctcgtcga
    10441 tctgcttcgt cagcgtggcg gcatcggccg gataccactg gccggcacag gccgccggcc
    10501 gaacggtcgc atccgcgcag gctgtcgcga ccagtagcgc caaaacgcag atcgggaggt
    10561 aaaccgggag gatcgaacga ggcatgcgca tggcgaaact cctctgggtc aacccgaaca
    10621 ttttaccaga ggagcgcccc gggcgcgcaa actcgcggga acccgtgcgt gacgagatca
    10681 ccgcgggaac gcatgacggg tg
//
LOCUS       DAOSYS010000119        10633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5312_length_10633_cov_5.350633, whole genome shotgun sequence.
ACCESSION   DAOSYS010000119 DAOSYS010000000
VERSION     DAOSYS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_10633_cov_5.350633"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="dacB"
                     /locus_tag="PLU99_08725"
     CDS             <1..1152
                     /gene="dacB"
                     /locus_tag="PLU99_08725"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOO17179.1"
                     /translation="GPDFCYVTRVYVRDDEVWVAGAGDPGLGDPRIAERCGQRPCEVF
                     DQWAADLKAAGVQRVARLVLDDSVFDETTRHPDWPSDQADRWYQAPVGGLNLNDNCLD
                     ARVEICDAPKLVLTPELPSDFILDSLRLGKKHKPRARRPYDSDRFEFSGYVCRSDELG
                     PISAGDPTVFFGYALKTALAKQGIVVGDIVRRSIPDPVAVGGRLVAEHSTSLPDVLWR
                     CNTFSQNLFAECLLKSLAAYGPDGRRTGQPGGWESGAAVVRATLSKLGVDLTGAVFRD
                     GSGLSHSNRASAAQIVQLLAVMKRHRHADVFVESLAVPGELGSMRRRYARIGPGGRLR
                     AKTGTLAEVHSLAGYVERADGTLLAFALLCNGPVSSELPVQVAEILADD"
     gene            complement(1314..2747)
                     /locus_tag="PLU99_08730"
     CDS             complement(1314..2747)
                     /locus_tag="PLU99_08730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOO17180.1"
                     /translation="MTGAGVLAILPLIIPSVAAVLLVLLISFRRSHFAAAAITLVGLA
                     LAFAATCWRPPPDAQQVTALLVMDGYAAFFNGLILAAAAATVLIAYGYLRAWGGMPEE
                     YYVLLLLATIGSQVLVASTHLISFFIGLEILSVSLYGLTAYPRTRLVSLEAGVKYLVL
                     AATSAAFLLFGMALVYAELGTMELGEIISRIADQPAGLVFSGGLALLVVGIGFKLGLV
                     PFHLWVADVYQGAPAPTTAFIATVSKGAIFALLVRYFVPLNALAGAAQFWMFGVLAIA
                     SMFAGNLLALLQNNLKRMLAYSSVAHMGYLMVAFLAGGELAVTAVSFYLVAYFITTLA
                     AFGVIAALSPGQREFESLSDYEALAWRQPWTAAVLSISLFSLAGIPLTAGFIGKFYVI
                     AAGANVRLWALLIILVANSAIGLYYYLRAILVMYRRPAEEQAGPAASAAPHVAARVVV
                     SVMTVLLLWFGLYPAPLIRLIQRLAAL"
     gene            complement(2744..4273)
                     /gene="nuoM"
                     /locus_tag="PLU99_08735"
     CDS             complement(2744..4273)
                     /gene="nuoM"
                     /locus_tag="PLU99_08735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOO17181.1"
                     /translation="MMLTLLILLLMLGGVAAWISGRWSRTWPRWISMGTALLHIIILL
                     VLWVHDLYRTEPGTAWLEETRRVWVPQLGITYHLAIDGLSLLLVLLSNFLGILAIASS
                     WEGIQKRVGFFHFNLMWILAALVGIFEAMDLFLFYFFWELVLVPLYFLIAIWGYENRY
                     YAAIKFFLFTQASGLFLLLAILGLYFAHARATGVYTFNYFDLLGTPIAGGAGFALMLG
                     FFVAFAVKLPVVPFHTWLPDAHTEAPTAGSVDLAGLVLKIGAYGMIRFIPPLFPQAAS
                     EFATFAMILGVIGIFYGAIVAFAQTDLKRLVAYTSISHMGFVLLGIFAWNELALQGAV
                     MVMIAHGLSTGGLFILVGDLQNRLHTRELSRMGGLWATAPRMGGVGLFLAMATLGLPG
                     LANFVGEIMVLFGVYQANVTLAAIATGGFVVATIYSVWMMQRVFFGPNVHELRLRDSN
                     AREMTIFGAVIAVTVWLGVHPAPVISTAKPALETLQDYARRPATTVSVRDAAPLERGE
                     S"
     gene            complement(4270..6132)
                     /gene="nuoL"
                     /locus_tag="PLU99_08740"
     CDS             complement(4270..6132)
                     /gene="nuoL"
                     /locus_tag="PLU99_08740"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOO17182.1"
                     /translation="MLGLLWLVPVLPLAAFVLLALGGARMPRAAVSALGVGLPAAAAV
                     IAALIAITFGASPPAGHSYTQVLWTWMPVAGADFMPQFALHLDALSIVMMLVVTWVGS
                     LILLYSSEFMEGEEGYSRYFAYMDLFISSMLFLVLARDLLVLFLGWEGVGLCSYLLIG
                     FWYRDENNVRAANKAFIVTRVGDTAFAIGLFLLFSSLGTLDIPEVLTRAAQQWPAGSA
                     VAAAAAALLLGGAVGKSAQLPLQTWLPDAMAGPSPVSALIHAATMVTAGVYLIARMHV
                     IFTLSPLVQWFVALVGALTLLLAACAALVQRDIKRVLAYSTISQIGYMFLALGVGAWT
                     AAIFHFMTHAFFKSLLFLAAGVVILAMHHEHNMFAMGGLRRRMPVTFWTFLAGGLALS
                     AFPLISAGFYSKDLILFEAFTLPRGNVLFWLAGFVGSLLTAIYTGRMVFLTFFGPAKH
                     EPARQPRWRMQTPLIVLGVLAICGGFINVPDFLRTALPAPPESGHSGLQIGLEFATGA
                     LVIVGILVAAALFVWRRAWADALYRLPGMAGVHAFLFSGWGFDWVYDHALVRPFVTLA
                     RINRRDFIDFFYTAVAWLNIGGHRVLSATENGRLRWYAAGIAAGAVIVIAIMVL"
     gene            complement(6134..6442)
                     /gene="nuoK"
                     /locus_tag="PLU99_08745"
     CDS             complement(6134..6442)
                     /gene="nuoK"
                     /locus_tag="PLU99_08745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017429976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOO17183.1"
                     /translation="MATVPLELALLLAGVLFALGLAGVLLRRNIIFVLLSIEIMLNAA
                     GLAFIVAGARWQQVDGQVMFLFILSMAAAEVAVGLALVLQMVHRFGTVDSDVVSRMRG
                     "
     gene            complement(6423..6959)
                     /gene="nuoJ"
                     /locus_tag="PLU99_08750"
     CDS             complement(6423..6959)
                     /gene="nuoJ"
                     /locus_tag="PLU99_08750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOO17184.1"
                     /translation="MEVAFYIAGIVAVIATLLAITRRNPVHALLYLVVSLLAVALVFY
                     VIGAPFIAALEVIIYAGAIVVLFLFVVMILGNVVVRPAEVDRHLQRPLTYVGPAVLGL
                     VLLGEFIYMLVRGAPGRTGPAAISPQELGEALFGPYLIGVEVAALLLLAGLVGAYYLG
                     RPKQPGEPEGETDGDRSA"
     gene            complement(6985..7521)
                     /gene="nuoI"
                     /locus_tag="PLU99_08755"
     CDS             complement(6985..7521)
                     /gene="nuoI"
                     /locus_tag="PLU99_08755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOO17185.1"
                     /translation="MLSVLRSMWSIPQSMWGVFLHMFGRRATIQYPEQKAPLPPRWRG
                     RIILSRDPDGGERCVACYLCSVVCPVDCIALQATTDETGRRYPEWFRINFSRCIFCGF
                     CEEACPTYAIQLTPDFEMSEYQRAAMVYEKEDLLISGVGKYPGYSFWRVAGVAIGGKD
                     KGQAEREKPPVDVRSLLP"
     gene            complement(7525..8481)
                     /gene="nuoH"
                     /locus_tag="PLU99_08760"
     CDS             complement(7525..8481)
                     /gene="nuoH"
                     /locus_tag="PLU99_08760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOO17186.1"
                     /translation="MSEGMRYALIIVIVLAALMSLAGLLIWVERRLLAVWQDRYGPNR
                     VGPFGLLQPAADIVKILFKQDWIPPFADKVVFCLAPLVIVATTLTSFAVVVFAPGIAV
                     ADLNIGLLFFLAMSSLGVYSIILGGWSSDNKYALLGSLRAAGQMLSYEVFMGLSLLGV
                     VMLAGSFSLGAIVESQRNVWFVVPQFLGFVIFVIAGFAETRRIPFDLPESENELVAGY
                     HAEYSGMKFAMFFLGEYIGITLISAMIVTLFFGGWLGPWLPPIVWFIIKTLIGLCVFI
                     LVRASLPRPRYDQLMAFAWKLMLPLSLLNLLVTGAIVVARGN"
     gene            complement(8478..>10633)
                     /locus_tag="PLU99_08765"
     CDS             complement(8478..>10633)
                     /locus_tag="PLU99_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOO17187.1"
                     /translation="FGRERDGVLESEFSGNLVEVCPTGVFTDKTLSRHYTRKWDLETA
                     PSICVHCGLGCNTIPGARYGELRRIRNRYNGEVNGYFLCDRGRYGYEFVNSQRRIRQP
                     LMRRSSGAVLETVTAEDALECARRLLTDRKRAIGIGSPRASLESNFALRTLVGPERFY
                     SGLSDRQAELISIALEVLQRGPVRTPTLREVELADAVLVLGEDVPNTGPRLALALRQA
                     ARRKGIKLAESLNVPEWNDAPVRDITQQDWSPLFIATPAATRIDDLATRTFRAAPDDL
                     ARLGHAVAHALDKRAAAPAGLPEEVAKLAAEIAAALRGAERPLVVSGVGCGDAGVVRA
                     AANVACALHAQGRAAALSLVVPECNSLGAGLLGGGDLSAARAALESGAADTLIVLEND
                     LYRRMTPAEADQLLNAARHVIVLEHTRTDTAERAELVLPVGTFAESAGTFVSSEGRAQ
                     RFYQVLPGQGAVRESWHWLGDLGQPWQFDELVAAIAAELPVFKRLPELAPPATFRIAG
                     QRVARDSHRYSGRTAMYADVSIHEPQPPQDPDSPLAFSMEGFHGQPPPPLVPRFWAPG
                     WNSVQSLNKFQSEVGGPLRGGDPGLRLIEPVPGTSVRFYDRPPAPFVVRANEVLVLPM
                     HHVFGSEELSSGSPGITMLSPTPYIAMRPQTARGLGLSEGDMTLVQVAGVEQRLPLKF
                     DDSLPAGAAGLTAGLTSRPEVPLPQWGTVRKAVPA"
BASE COUNT         2278 a         3625 c         3098 g         1632 t
ORIGIN      
        1 gggcccgact tctgctacgt cacacgcgtc tacgtaaggg atgacgaggt gtgggtcgcc
       61 ggtgcaggcg atccggggct gggcgatccg cgcatcgcgg aacgctgcgg gcagcgcccg
      121 tgcgaggtct tcgaccagtg ggccgccgat ctgaaagccg ccggcgtaca gcgcgtggcg
      181 cgcctcgtac tggacgatag cgtcttcgat gagaccactc gccatccaga ctggccaagt
      241 gaccaggccg accgctggta tcaggcgccc gtcggcggcc tgaacctcaa tgacaactgc
      301 ctcgacgcgc gggtcgaaat ctgcgacgcc ccgaaactcg tgctgacgcc ggagctgccg
      361 tccgacttca tcctcgactc acttcgccta gggaagaagc acaagccgcg cgctcgccgg
      421 ccctacgata gcgaccgctt tgagttcagc ggttacgttt gtcgcagcga cgagttgggg
      481 ccgattagtg ccggcgaccc gaccgtgttc ttcggctacg ccttgaagac cgcccttgcc
      541 aagcagggca tcgtggtggg cgacatcgtg cgccgctcca taccggaccc cgtcgccgtc
      601 ggaggccgcc tagttgccga gcattcgact agcctgccgg acgtcctatg gcgctgcaat
      661 accttcagcc agaacctgtt cgccgagtgc ctgctgaaga gcctggcggc ctacgggccg
      721 gacggacggc gaaccggaca gccgggcggc tgggaatccg gggcggctgt cgtgcgtgcc
      781 acgctgagca agctgggcgt ggacctgacc ggggccgtat ttcgcgacgg cagcggcttg
      841 agccattcta accgtgcgtc ggccgcgcaa atcgtgcaac ttctggcggt catgaagcgg
      901 caccggcacg ccgacgtttt tgtggagagc ctggcggtgc ccggcgaact cggctcgatg
      961 cgccggcgtt acgcccggat cggccccggc ggcaggctgc gggcgaaaac cggcacgttg
     1021 gccgaagtcc attcgctggc cggttatgtc gagcgcgcgg acggaacgct gctggcgttc
     1081 gccctgctgt gcaatggtcc cgtgtccagt gaactgccgg tgcaagtggc cgaaatactc
     1141 gccgacgact gagccggtcc gctcaggatt gtcggtgggg tttgttcagc gcacaccttg
     1201 tgagcacgtc agtgtgcaga gcagagcaca accggggcgt gcagccggaa taccgccgac
     1261 gggccccatc gccgccgttg actcaaaacc attgctgctt gcctcggccc gcgctacagc
     1321 gctgccaagc gttgtattaa ccgaatcagc ggcgccggat acagcccgaa ccacaacagc
     1381 aataccgtca tgacgctaac cacgacgcgt gcggcaacgt gcggcgccgc gctggcggcc
     1441 gggccggcct gttcctctgc cggccgccga tacatgacca gaatggccct taggtagtaa
     1501 tacagtccga tggcgctgtt cgcgaccaga atgatcagca gcgcccaaag ccgcacattc
     1561 gcccccgccg caatgacgta gaacttccca atgaaccccg ccgtcagcgg aatgccggcc
     1621 agcgagaaca gactgatgct caggaccgcc gcagtccacg gctgccgcca cgccagcgct
     1681 tcgtaatcac tgagtgactc gaattcgcgc tggccgggcg aaagcgcggc tatgacaccg
     1741 aaagcggcca gcgtcgtaat gaagtaagcc accaagtaga acgacaccgc cgtgacggcc
     1801 agttcgccgc cggccaggaa cgccaccatc agataaccca tatgtgcgac ggacgagtag
     1861 gccagcatgc gcttcagatt gttctgcagc agcgccagca gattgccggc gaacatcgag
     1921 gcaatcgcca gcacgccgaa catccagaac tgcgccgccc cggccagagc gttcagcggc
     1981 acgaaatagc gcaccaacag tgcgaatatc gccccctttg ataccgtcgc gataaaggcc
     2041 gtcgtcggcg ccggtgcgcc ttgatacacg tccgccaccc acaggtgaaa cggcaccagc
     2101 cccagcttga agcctatccc cacgactagc agcgccagcc cgcccgagaa caccagcccc
     2161 gccggctgat cggcgatccg gctgatgatc tcgcccagtt ccatcgtccc cagctcggca
     2221 tagaccagag ccatcccgaa cagcagaaac gcggccgatg tagccgccag caccaagtac
     2281 tttactcccg cttccaaact gacgagccgt gtgcgcggat aggcagtcag cccatacagc
     2341 gagacgctga gtatctccaa ccctatgaag aacgagatta aatgcgtact ggcgacgagc
     2401 acctgcgacc cgatcgtcgc caaaagcagc agcacgtagt attcctcagg catcccgccc
     2461 caggcgcgca gatagccgta ggcgatcaac accgtcgcgg ccgccgcggc caaaatcagc
     2521 ccgttgaaga atgcggcata gccgtccatc acaaggagcg ccgtcacctg ctgggcgtcc
     2581 gggggcggcc gccagcaggt cgccgcaaaa gccagcgcca agccgaccag cgtgatcgcc
     2641 gccgccgcga aatggctgcg ccgaaacgag atcaagagca ccagtagtac ggcggcaacc
     2701 gatggaatta tgagcggcaa tatcgcgagt acgccggcgc cggtcatgat tcgcctcgct
     2761 cgagcggcgc cgcgtcgcgg actgacacag ttgtcgccgg gcggcgcgca tagtcctgta
     2821 gagtctccaa cgcgggcttc gcagtcgaaa tgaccggagc aggatggact ccgagccaca
     2881 cggtcaccgc gatcacggcg ccaaaaatcg tcatctcgcg cgcgttcgaa tcacgcagcc
     2941 gcagttcatg cacattggga ccgaagaaga cccgttgcat catccagacc gagtaaatgg
     3001 tcgcaaccac gaacccgccg gtggcgatgg ccgccagcgt gacattggcc tggtacaccc
     3061 cgaacaggac catgatttcg cccacgaaat tcgccagtcc cggcagtccc agcgttgcca
     3121 tggccaggaa cagcccgacg ccgcccatgc gcggggcggt ggcccacagc ccgcccatgc
     3181 ggctaagctc acgcgtgtgc aaacggttct gcagatcgcc gacgagaatg aacaggccgc
     3241 ccgtgctcag gccgtgcgcg atcatgacca tcacggcgcc ctgtaacgcc agctcattcc
     3301 aggcaaagat gcccagcagc acgaacccca tatgacttat gctggtgtag gctacgaggc
     3361 gcttcaggtc cgtctgggcg aatgccacta tggccccgta gaagatgcca atgaccccga
     3421 ggatcatggc gaacgtagcg aattcggaag ccgcctgcgg gaacagcggc ggaatgaacc
     3481 ggatcatccc gtaggccccg atcttgagca ccagcccggc caggtcgacg ctgccggctg
     3541 tcggcgcctc ggtgtgtgca tccggcagcc acgtatgaaa cggaacaacc ggcagtttga
     3601 ctgcaaacgc gacgaaaaag cccagcatca gggcgaaacc agcgccgccg gctatgggcg
     3661 tgccgagcag atcgaagtaa ttgaaagtgt atacgccggt ggcccgggcg tgggcaaagt
     3721 acaaacccag aatcgcgagc agcaggaaca gcccgctggc ctgtgtaaac aggaagaact
     3781 tgatggccgc ataatagcgg ttttcatacc cccaaattgc gatcagaaaa tacagcggca
     3841 ccagcaccag ttcccagaag aagtagaaca ggaacaggtc catcgcctcg aatatgccga
     3901 ccagtgcggc cagtatccac attagattga aatggaagaa gccgacgcgc ttttgaatgc
     3961 cttcccaaga ggaggcaatc gccagaatgc cgaggaaatt gctcagcagc accagcagca
     4021 gactcaagcc gtcgatcgcc aggtggtaag tgatgccgag ttgcggcacc cagacgcggc
     4081 gcgtctcctc cagccaggcg gtcccgggct cggtgcggta caggtcgtgg acccacagca
     4141 cgagcagaat gatgatgtgc aacagggcgg tccccattga aatccagcgc ggccaagttc
     4201 ggctccagcg accggagatc caggccgcca ccccgcccag catcaacaac agaatcagca
     4261 gcgtcagcat cacagtacca tgatcgcaat gacgataacc gccccggcgg cgatcccggc
     4321 ggcgtaccag cgcagccgcc cattctcagt tgcgctgagc acacggtggc cgccgatgtt
     4381 cagccaggct acagcggtat agaagaagtc tataaagtca cgccgattaa ttcgcgccag
     4441 cgtcacaaac ggccggacca gggcgtgatc gtatacccag tcgaatcccc acccgctgaa
     4501 caggaatgcg tgcacaccgg ccatgcccgg caaccggtac agggcgtcgg cccaggcgcg
     4561 ccgccagacg aacagcgcgg ccgccacgag gatccccacg atcaccagcg cgccggttgc
     4621 gaactccagc ccgatttgga gcccgctgtg tcctgactcc ggcggagccg gcaaagccgt
     4681 cctaaggaaa tccggaacat tgatgaagcc gccgcagatt gccagcacgc cgaggacgat
     4741 taacggcgtc tgcattcgcc agcggggttg ccgtgcgggc tcgtgctttg ccgggccgaa
     4801 gaacgtcagg aataccattc gccccgtgta gatggctgtc aatagcgatc ccacgaatcc
     4861 cgcgagccag aacagaacat tgccgcgcgg cagtgtaaac gcttcaaaca ggatcaagtc
     4921 cttgctgtag aacccggcgc tgatcagcgg aaacgcggaa agtgccaacc cgcccgccaa
     4981 gaaagtccag aacgtcaccg gcatgcgccg ccgcaagccg cccatggcga acatgttgtg
     5041 ctcgtgatgc atcgccagaa tcaccacgcc ggccgcaaga aacagcagag atttgaaaaa
     5101 cgcgtgcgtc atgaagtgga aaatcgcggc cgtccacgcc cccacgccca acgccaggaa
     5161 catgtaccca atctggctga tagtcgaata cgccaggacg cgcttgatgt ctctctgcac
     5221 cagagccgca caggccgcca gcagcagcgt gagtgcccca accagcgcga caaaccactg
     5281 caccagcggc gaaagcgtga agatgacgtg catgcgggca atcaggtaca cgccggccgt
     5341 caccatcgtc gcggcgtgaa tcagcgcgct caccggcgag ggaccggcca tcgcgtccgg
     5401 caaccaggtc tgcagcggca actgtgccga tttccccacc gcgccaccca acagcagcgc
     5461 cgccgcggcc gccgctacgg ccgagccggc cggccactgc tgcgccgctc gggtcagcac
     5521 ttccggaatg tcgagcgtac caaggctgct gaacagcagg aacaagccga ttgcaaaggc
     5581 ggtgtccccg acgcgcgtga ctataaaagc cttattcgcc gcgcggacat tgttctcgtc
     5641 cctataccag aacccgatca gcaggtagct gcacagtccg accccttccc agcccaggaa
     5701 cagcaccagc agatcgcggg ccagcaccag gaagagcatc gagctgataa acaggtccat
     5761 gtacgcgaaa taccgcgaat agccctcctc gccctccatg aactccgatg agtacagcag
     5821 tattagagag ccgacccacg tcacaaccaa catcatgacg atcgacagcg cgtccaggtg
     5881 cagcgcgaac tgcggcatga aatccgcccc ggcaaccggc atccacgtcc acaatacctg
     5941 tgtgtaggag tgcccggccg gcggggatgc gccgaacgtt attgctatga gcgccgcgat
     6001 tacggccgcc gccgcgggca atccgacgcc gagcgcgctg accgcagccc gcggcattcg
     6061 tgccccgccc aatgcgagca gcacaaaggc cgccagcggc agaaccggaa ccagccacag
     6121 cagcccgagc atcctatcct ctcatccggc tgaccacgtc ggagtccacc gtgccaaacc
     6181 gatgcaccat ttgcagtact agcgccagtc caaccgccac ttccgccgca gccatggaga
     6241 gtatgaaaag gaacatcacc tgcccgtcca cctgctgcca gcgcgccccc gcgacaatga
     6301 aggctagccc ggccgcgttc agcatgattt cgatcgatag cagcacgaag attatgttgc
     6361 gccgcagtag cacccccgcc aggccgaggg caaagaggac acccgccagc agcagggcca
     6421 actcaagcgg aacggtcgcc atctgtctct ccctcaggct cgccgggctg tttgggccgg
     6481 cccaggtaat aagcccccac caagcccgcc agcagcagca atgcggccac ttccacgcct
     6541 atcaagtaag ggccgaatag cgcctcgccc agctcctgcg gactgatggc ggcgggcccc
     6601 gtccgccccg gcgctccgcg caccagcatg taaatgaact cacccagtag caccaatccc
     6661 agtactgccg ggccgacgta tgtcagcggc cgctgcaggt ggcggtccac ttcggctggg
     6721 cgcacaacca cattgcccaa tatcatcacc acaaacagaa atagtacgac aattgcgccg
     6781 gcatagatga tcacttcgag cgctgcgatg aatggtgccc cgatgacata aaacaccaga
     6841 gcgaccgcca gcaacgacac caccaggtat agcaaggcgt ggactggatt acggcgcgta
     6901 atcgccaata aggtggcgat aaccgccacg atgcccgcta tgtagaaagc tacttccatg
     6961 ccaactccgc gcttgcctgc cagcttaagg caacaagctg cgcacgtcca ccggcggctt
     7021 ctcccgctcg gcctgcccct tgtctttgcc tccgatcgcc acgccggcca cgcgccagaa
     7081 gctgtagcct gggtacttgc cgacgccgct gatcaacagg tcctcctttt cgtaaaccat
     7141 cgccgcccgc tggtattcgg acatctcaaa atcgggcgta agctgaatgg cgtaagtcgg
     7201 acacgcctcc tcacagaacc cgcagaaaat gcaacgcgaa aagttaatgc ggaaccactc
     7261 cggatagcgc cggcccgttt cgtccgtggt cgcctgcagc gcaatgcagt ccaccggaca
     7321 gaccacggag cacaaataac acgccacgca gcgctcaccg ccgtcggggt cgcgcgacaa
     7381 gatgatccgg ccgcgccaac ggggcggcag cggggccttc tgttcggggt actgaatagt
     7441 cgcgcgccgc ccgaacatgt gcaggaaaac gccccacatc gactgtggta tgctccacat
     7501 tgaccgcagt acgcttagca tgctctaatt gcccctggcg actacgatgg cgcccgtcac
     7561 cagcagattc aatagactta aaggcagcat cagcttccac gcaaaggcca tgagctggtc
     7621 gtagcgcggc cgcggcagcg acgcgcgcac cagaataaat acgcacaatc cgatcagcgt
     7681 cttgataatg aaccagacga tcggcggcag ccacggaccc agccagccgc cgaagaacag
     7741 cgtgacgatc atcgcggaaa tcagcgtgat tccgatgtac tcgcccagga aaaacatggc
     7801 gaatttcatg cccgagtact cggcgtgata gccggccacc agttcgttct cggattccgg
     7861 caggtcaaag ggaatgcggc gcgtctcggc aaaacccgcg atgacgaata tcacaaagcc
     7921 gaggaactgc ggaacgacga accagacatt ccgctgggac tcgacgatcg cccccaggct
     7981 gaaagacccc gccagcatta cgacgcccag cagcgaaagc cccatgaata cttcgtagct
     8041 gagcatctga cccgcggccc gcaacgaccc gagcaaggcg tatttattgt ccgacgacca
     8101 gccgcccaga atgatgctgt atactccgag cgaggacatc gccagaaaaa acagcagccc
     8161 gatgttcagg tccgcgacag caattcccgg cgcgaacact acaaccgcaa acgaagtcag
     8221 tgtcgtcgct acgatgacca gcggcgccag acaaaagacg actttgtccg cgaagggcgg
     8281 aatccagtcc tgcttgaaca gaattttcac gatgtcggcc gcgggctgca gcaatccgaa
     8341 gggccctacg cggttgggac cgtagcgatc ctgccatact gccagcaaac gccgctcgac
     8401 ccaaatcagc agtccggcca gcgacatcag cgccgccagc acgataacta taatcaaggc
     8461 gtagcgcatg ccctcgctca tgccggcacc gcctttcgca ctgtgcccca ctgcggcaaa
     8521 ggcacctccg gccggctcgt aagcccggct gtcagtccgg ccgcgcccgc cggcaggcta
     8581 tcgtcgaatt tcaacggcag ccgttgctcg acgcctgcga cctgcaccag tgtcatgtca
     8641 ccttcgctaa gccccaagcc gcgcgctgtt tgcggccgca tggcgatgta aggcgtgggg
     8701 ctcagcatcg ttatgcccgg cgatccgctg cttagttcct ccgagccgaa aacatggtgc
     8761 attggcaaca ccagaacttc gttcgcccgc acgacgaatg gcgcgggcgg ccggtcgtag
     8821 aaccgcacac tcgtgcccgg caccggctcg atcagccgca gcccgggatc gccgccgcgc
     8881 agcggcccgc cgacttcact ctggaatttg ttcagcgatt ggaccgagtt ccaacccggt
     8941 gcccagaagc gcggcaccaa cggcggcggc ggttggccgt ggaacccctc catgctgaac
     9001 gccagcggcg aatccgggtc ttgcggcggc tgcggctcgt gaatgctgac gtccgcgtac
     9061 attgccgtcc gtccgctgta gcggtgcgaa tcacgcgcca cgcgttgccc ggcaatgcgg
     9121 aaggtcgccg gcggggcaag ctccggcaac cgcttgaaca ccggcagctc cgccgcaatc
     9181 gccgctacca gttcgtcaaa ttgccatggt tggcccagat cccccaacca atgccagctc
     9241 tcgcgcaccg ccccctgccc gggaagcact tggtagaagc gctgggcgcg gccctcgctg
     9301 ctgacaaatg tgccggctga ttcagcgaat gtgccgaccg gcaacaccag ttcagcacgc
     9361 tcggccgtgt cggtgcgcgt atgttcgagc acaatgacgt gccgtgccgc attcagtagc
     9421 tggtccgctt cagccggcgt catcctgcga tacaaatcgt tttcgagcac aatgagcgtg
     9481 tccgccgcgc cgctctccaa tgccgcacgg gcggcactca gatcgccccc tcctaatagt
     9541 ccggcaccca gactgttaca ttccggcaca accaggctca aggccgcagc ccgcccttgc
     9601 gcgtgaagcg cgcaggcaac attcgcggcc gcccgaacta ccccagcgtc gccgcagcca
     9661 acgccggaca cgaccagcgg ccgctcggca ccccgtagtg cggccgcaat ctccgccgcg
     9721 agtttcgcaa cttcctcagg cagacccgcc ggcgccgcag cgcgcttgtc cagggcgtgc
     9781 gccacggcat gccccagccg cgccaggtca tcaggcgccg cacgaaacgt tcgcgttgcc
     9841 aagtcatcaa ttctggtggc ggcgggcgtt gcgatgaaca gcggactcca gtcctgttgt
     9901 gttatgtcgc ggacgggcgc atcgttccac tccggcacgt ttagactttc ggccagtttg
     9961 atgcccttgc ggcgcgctgc ctgccgtaac gccagggcca gccgcggccc ggtattcggc
    10021 acatcctcgc ccagcaccaa cacggcgtcc gccaattcga cctcgcgcag cgtgggcgtg
    10081 cgcaccggcc cgcgctgcag cacctccagc gcaatggaaa tgagttccgc ctgccggtcg
    10141 gacagcccgc tatagaaacg ttccggcccg accagcgtac gcagcgcgaa atttgattcc
    10201 agcgacgccc gcggcgagcc gatcccgata gctcgcttcc ggtccgtcag caaccgccgc
    10261 gcgcattcca acgcatcttc tgcggttacc gtttccaaga ccgccccgct cgacctgcgc
    10321 atcaacggtt gccggatgcg gcgctgcgag ttgacgaatt cgtagccgta ccgcccgcga
    10381 tcgcacagga aatacccgtt gacttcgcca ttatagcgat tgcgaatccg ccgcagctcg
    10441 ccatagcggg cgccgggtat ggtattgcat cccagtccac aatggacgca aatagacggc
    10501 gccgtttcga ggtcccactt gcgcgtatag tgccgggaca gcgtcttgtc cgtgaacaca
    10561 cccgtcgggc acacctcgac gaggttgccg ctgaactcgc tttcgagcac gccgtcgcgc
    10621 tcgcgcccga agt
//
LOCUS       DAOSYS010000120        10545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5361_length_10545_cov_6.223260, whole genome shotgun sequence.
ACCESSION   DAOSYS010000120 DAOSYS010000000
VERSION     DAOSYS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5361_length_10545_cov_6.223260"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PLU99_08770"
     CDS             complement(<1..1746)
                     /locus_tag="PLU99_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOO17188.1"
                     /translation="MTARAETAAQKLPALLPSLLLCAALVAGIVCLRLLVPSDRPLAI
                     AYGLPLLVCLWVRRRWVLWSMAGALLLAALGKTFFHLDTTEPLLQFGMRAASILTLAG
                     VIHAVIALRERLEARSAELERSNAELARSNEELRVREEEIAQQNEELQSQTEELEQQS
                     EELLTQTEELQRANEELTRRERVLQNLVEVARSFSGEGLPLSEVCRLALPLVGQPAAA
                     VAILERGPEGQVVIRGHYGFGRAGPQRAGWNFEDSFAALIMERNETGYLEDAAQRSDL
                     SLTQRAEGEPFCSILATPLRLHGKPIGVVEVLAAEPCQWTIEQFRLVEWVAAECSLLI
                     ETLRLQDELEARRREAEEASLRKTRFLAAVSHDIRTPVNAITLMAELLTRAVADPHRT
                     EDLAELAADLKANARGLVELVSDVLDIARFDSGRLELEISEFPLLPLLQGEMRRLSPV
                     AEAKGLRLHIEPLQSDVRLRTDRVKLTRVLNNLIGNAIKFTEEGEVRICPEPMNGGLH
                     IHVSDTGVGIPAEHLPRIFDEFFQLKNPARDRSKGTGLGLAICRRLVEAIGAELTVDS
                     ILGAGSTFTLRLPPAL"
     gene            complement(1743..2618)
                     /locus_tag="PLU99_08775"
     CDS             complement(1743..2618)
                     /locus_tag="PLU99_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOO17189.1"
                     /translation="MQTINDRFRNVRFEGRTLRPLKWQRPLPQGAIAAEDLRFLADLF
                     EQAGLELQGYRLSSLQRRMRACCRALRVPNATEARALLGRAPHMVPRALDAVVLGVSE
                     FFRDPAVFEELRTRVIPELAAVSRPLRVMSIGCADGRELYSVAILLADGNLLDDCSLL
                     GIDCREDAIAQARAGLFSPAALERVPDEWRERYFAGRGVLFEISSAIRRRTTWCVGNL
                     FKLPPLPACDLLLFRNVSIYLEPNGAAQAWRCILPLVRPGGAVVLGKAERQPETSGLT
                     KIGSSIYRRVSGDSN"
     gene            complement(2894..3988)
                     /locus_tag="PLU99_08780"
     CDS             complement(2894..3988)
                     /locus_tag="PLU99_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HOO17190.1"
                     /translation="MKIHKDHQRFRQIVKGKIRADLRRFLTRGELIGKEGKHLVSIPV
                     RGIDLPHFRYGDNNDSGVGQGEGQPGQAVDGDESGIGPGGTDPGRHILEVDVSLDELA
                     DILGEELQLPRIQPKGRRNITAVKDRYSSVRQSGPESLRHFKRTFRRALRRMIMTGMY
                     DPHKPAIVFERADKVYRSWKPVRKPDSNALIVYMMDISGSMGHEQKELVRLEAFWIDA
                     WLRRNYDGIESRYIVHDVQAGEVDRETFFHIREDGGTKISSAFKFCRDMLDKHYNTDE
                     WNIYLFHFSDGDNSSEADSRECCKILQDSLLPRANQFGYCQVASAYGSGAFINTLRDQ
                     LGHAENVVTSRVNTKDDIYDSIKEFFKKGR"
     gene            complement(4079..6139)
                     /locus_tag="PLU99_08785"
     CDS             complement(4079..6139)
                     /locus_tag="PLU99_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HOO17191.1"
                     /translation="MAKRDELLEIVDRHLDSESFRQQHWEGTFWEYLDMVLANPRIAR
                     NAFQRIYDMILYSGTQRYTSMKQEYVHYKFFDDPFDNGADAVYGLDGALMRLVDCFKS
                     AAQGYGTDRRILLLHGPVGSSKSTIVRLLKKGLEHYSRLDEGALYSFAWKGIGDANEA
                     EVATCPMHEEPLKLIPRRARRDILAKLNADLPEDRQVRVDGDLDPFCRKTFEDLLIRY
                     KGDWRAVMDHVVVRRVVLAEKDRVGIGTFQPKDEKNQDSTELTGDINYRKIALYGSDS
                     DPRAFNFDGELNISNRGICEFIEILKLDVAFLYDLLGATQEHTIKPKKFAQTDIDEVI
                     IGHTNEPEYKKLQSNELMEAFRDRTIKIDIPYNIRLTDEIKIYGKDFGRDKVKGIHIA
                     PHTVEMAAMWAVLTRLEEPKKAGLSLLQKLKLYDGKSIPGFTEDSIRELREEAHREGM
                     QGISPRYIQDKISNALVSEQAQEDRAINPFMVMNELENGLSHHSLISDEELKKRYKEL
                     LAVVREEYEDILKTEVQRAISADEDAIARLCTNYIENVRAYTQHERVRNKYTGRDEEP
                     DERLMRSIEEKIDIPESRKDDFRQEIMNYIGALALDGKKFEYHTNARLHKALELKLFE
                     DSRDSIKLKNVVSGVVDDETQAKIDVVKQRLIKFFGYNETSATDVLNYVASIFARGDA
                     KQRD"
     gene            complement(6339..6824)
                     /locus_tag="PLU99_08790"
     CDS             complement(6339..6824)
                     /locus_tag="PLU99_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17192.1"
                     /translation="MIEVRLGRRPQKLTSYRPMLERRGDVELNAYARHMRRNVLGILL
                     GLVLIGGAAWLYHAFSSQDRVAPQPRAYSDKLRCGECGHEQLVQVSTRDKFPLTCPKC
                     KAHDCWKVWRCAECVTEFVSKVGREDRETAATILRCPKCGSMSVGSAARPPVPAPAAQ
                     P"
     gene            6989..7483
                     /gene="ispF"
                     /locus_tag="PLU99_08795"
     CDS             6989..7483
                     /gene="ispF"
                     /locus_tag="PLU99_08795"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOO17193.1"
                     /translation="MTSALSFRTGIGYDLHRLTHGRPLVLGGVRIPHARGLRGHSDAD
                     VVLHALCDALLGAAGLGDIGELFPDSDPKWAGADSRLFLANVLARVAAAKFRIVNADI
                     IIHAQEPRLGPLKADIQRSLSELLNLPPGAVGVKATTNEGLDAVGRAEAIACWAAVLL
                     AGTA"
     gene            7583..8569
                     /locus_tag="PLU99_08800"
     CDS             7583..8569
                     /locus_tag="PLU99_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17194.1"
                     /translation="MITQSSVSPPPPAVVVRNLEKHFGDFVAVHDVSFEVAAGQIFGL
                     LGPNGAGKSTTIRMLCGILTPTAGSGTVAGLDMLTQPELIKARIGYMSQRFSLYEDLT
                     VAENIDFYAGIYGLSRARRRERKEWVIEMAGLQEHRRSRTAILSGGWKQRLALGCAIL
                     HEPPVVFLDEPTAGVDPISRRRFWDLIYGMAAGGVTVFVTTHYMDEAEYCDRIGLVYR
                     GELIATDTPERLKTQLHDEDVLEVECDRPQDALAILEQVPGVREAALFGRGLHVVAAR
                     GPATIQAVQSTLAAHAFGVTRVTRIAPSLEDVFVSLIEARDRQEAPQAEVRE"
     gene            8658..10292
                     /locus_tag="PLU99_08805"
     CDS             8658..10292
                     /locus_tag="PLU99_08805"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOO17195.1"
                     /translation="MGQNRPKGVKPRDPAKEPAVQQIASFVGFFIYLLVLKSFFLPLF
                     VIPTGSMAETLYGAHAIHTCPNCGFEYPIGISPPPHREIPIIRCPNCGWQEFTDSPNL
                     MQRYRTPFLRIDGSIDSPLHTRGGDRIMVHGWAYDLGGRYAPQRWDVVVFKVPSDLTT
                     NYIKRLIGMPGEQIEIIDGDIWVAPPGGKLAIAHKPPQVQEALWFSFYNHDFPPAGPS
                     QDGRYHPRWITVGNSDGWQGLDTRFPSFNGEPGENGRIMFVTEAGATMNPGRITDVYG
                     YDRPAARDNRGNLRPVEPHIVSDVRLSCRVDFDSDAPPETFVELSATKDGDSFFGRIY
                     ADGRLMLEHVGKRGVRESWGEARLKRPGRPVVFALASVDYTVSLQVDGEVTLQSTPEQ
                     YHVDAAQARAHSEAAISPQLLITAGGGPVHLAHLLIERDVYYTSDLGTGTAGYGTQRH
                     PITLGPNDYFMLGDNSPYSQDSRYSFVSSPDEPLLASYVNPDYQRGTVPGDQLIGPAF
                     LVYWPGMGQLLPERYMSGALRHLNALPTPGRIRWIR"
     gene            10327..>10545
                     /locus_tag="PLU99_08810"
     CDS             10327..>10545
                     /locus_tag="PLU99_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOO17196.1"
                     /translation="MNSLPPENSPAVRTSPAPIGYSPSQATRIVETLQTVLTALLLAF
                     VFRAFLVEAFIIPTKSMAHSLLGAHATRT"
BASE COUNT         1884 a         3540 c         3036 g         2085 t
ORIGIN      
        1 taacgccggc ggcaatcgca aggtgaacgt gctccccgcg cccagaatgc tatcgaccgt
       61 cagctcggcg ccgatcgcct ccaccagccg gcggcagatc gccagcccca gtccggtgcc
      121 cttgctgcgg tcacgggccg gattcttcaa ctggaagaac tcgtcgaaga tgcgcggcag
      181 atgttccgcc ggtatgccaa cgcccgtatc agaaacgtgg atatgcaagc cgccgttcat
      241 cggctcggga caaatgcgaa cctcgccctc ttcggtgaac tttatggcat tgccaatcag
      301 gttgttcagc acgcgcgtca gcttcacccg gtcggtgcgc agccgcacgt cgctttgcag
      361 cggctcgatg tgcagccgca agcccttggc ctccgccacc ggcgaaaggc gccgcatttc
      421 gccctgaagc aggggcaaca acgggaattc gctgatttcc agttcgagcc gcccgctgtc
      481 gaaacgcgca atgtccaaca cgtccgagac aagttccacc agtccgcgcg cgttggcctt
      541 caggtctgcc gctagctccg ccaggtcttc ggtgcggtgc gggtcagcca ccgcccgcgt
      601 cagcagctcg gccatcagcg tgattgcgtt caccggcgtg cgaatgtcgt gcgaaaccgc
      661 cgccaggaac cgcgttttgc ggagcgaggc ctcctcggcc tcccgccgcc gcgcctcaag
      721 ctcgtcctgc aagcgcaatg tttcgatcag cagtgaacat tcggccgcca cccactcgac
      781 cagccgaaac tgctcgatcg tccactggca cggttcggcc gccagcacct cgaccacgcc
      841 gatgggcttg ccgtgcaggc gcagcggagt ggccaggata gagcagaatg gctcaccctc
      901 ggcccgctgc gtgagactca gatccgaacg ctgcgcggcg tcctcgagat acccggtttc
      961 attccgctcc attatcaaag cggcaaacga atcctcgaaa ttccaaccgg cgcgctgcgg
     1021 accggcccgt ccaaaaccgt aatgcccgcg gattacaacc tgaccttccg ggccacgctc
     1081 caggatggcg acggccgcgg ccggctgccc caccagcggc aaggccaatc gacacacttc
     1141 cgaaagtggc agaccttctc cggaaaagga gcgtgccacc tcgaccaggt tctgcaagac
     1201 tctttcgcgg cgcgtcagct cctcgttggc tctctgcagt tcctcggtct gcgtgagtaa
     1261 ctcctcgctc tgctgctcca gctcctcggt ctgcgattgc agttcctcat tctgctgcgc
     1321 gatttcctcc tcccgcaccc gcaactcctc gttactgcgg gccaactcgg cgttggaacg
     1381 ctccagctcc gcgctgcgtg cttctaaccg ctcgcgcagg gcgattaccg cgtgtatcac
     1441 gccggccagc gtaaggatac ttgcggcgcg catgccaaac tgaagcagcg gctccgtcgt
     1501 gtccaggtgg aaaaatgttt ttcccaaagc ggccaacaaa agcgcgccgg ccatgctcca
     1561 cagtacccag cggcggcgca cccaaaggca gactagcagc ggcagaccgt aagcgatggc
     1621 cagcgggcga tccgacggca ctaacagccg gaggcagact atccccgcca ccaaggccgc
     1681 acatagcagc aggcttggca gaagcgctgg gagcttctgg gccgccgttt cagcgcgagc
     1741 ggtcaattcg agtctcctga tacacgtctg taaatactgg agccgatctt cgtcagccca
     1801 cttgtttcgg gctgacgctc ggcctttccc agcacgaccg caccgcccgg gcggaccagc
     1861 ggcagaatgc aacgccacgc ttgggccgcg ccattcggct ccaggtaaat actcacattt
     1921 ctgaacagca gcaggtcgca cgccggcagc gggggcagct tgaacaagtt gcccacgcac
     1981 caggttgtcc gcctgcgtat cgcggaactg atttcaaaca gcacgccccg cccggcaaag
     2041 tagcgctccc gccactcatc cggcacgcgc tccaacgccg cggggctgaa taagccggcg
     2101 cgggcttgcg cgatcgcgtc ctcgcggcaa tcgatcccca acaagctgca atcatcaagc
     2161 aggttgccat ccgccaacag gatcgcaacg gagtacagct cgcgcccgtc cgcgcacccg
     2221 atgctcatca cacgcaaggg cctgctgact gcggccagct ccgggatgac gcgcgttcgg
     2281 agctcttcaa acaccgccgg gtcgcgaaaa aactccgaca cgccgagcac cacggcatcc
     2341 agcgcacggg gaaccatgtg cggcgcgcgg cccaagagcg cccgggcctc agtcgcgttt
     2401 ggcacgcgta gcgcccggca gcaggcccgc atccgcctct gcagcgacga tagccgatag
     2461 ccttgcagct ccaggccggc ctgctcgaac aagtccgcca gaaacctcag gtcttccgcg
     2521 gcaatagcgc cctgcgggag cgggcgctgc catttcagcg gccggagcgt tcgcccctcg
     2581 aatcgcacgt tgcggaatct gtcgttgatg gtttgcactt ccttctttct tcgaacgtcg
     2641 gccggctgcc tacgctaaaa ggcccgtcac gttacatcca ataaacagga tagcaccttg
     2701 tgtacatgct ttcaggcttg cccataaatt taagagattc cagcttacga acgacttaca
     2761 gcgtgattac attggcgtca tgcatcgcgc tacaccggca ggcgcgtcgc ccggaaaggc
     2821 cgccaggatt tcccccatgg cgcgcaggcg tgtccccccg ctcgtcttat aggccccccc
     2881 ttaccgcccc ttcttaccgg cccttcttga agaattcttt gatgctgtcg tagatgtcat
     2941 ccttggtgtt aacacgggaa gtgacgacgt tctcggcatg cccgagttga tcgcgtagcg
     3001 tgttgatgaa cgctccgctg ccgtacgcac tggctacctg gcaataaccg aactggttcg
     3061 cccgcggcag cagcgaatcc tgcaaaatct tgcagcattc gcgcgagtcc gcctcggatg
     3121 agttgtcgcc gtctgagaag tggaacaggt agatattcca ctcatccgtg ttgtagtgct
     3181 tgtccagcat gtcgcggcag aatttgaacg cactggagat tttggtcccg ccgtcctcac
     3241 gaatatggaa gaaagtctcg cgatcgacct cgccggcctg cacgtcgtgc acgatgtacc
     3301 ggctttcgat cccgtcgtag ttccgccgca gccaggcgtc aatccagaat gcctccagcc
     3361 gcaccagctc cttctgctcg tgccccatcg acccggagat gtccatcatg tagacgatca
     3421 gcgcgttcga atccggcttg cgcaccggtt tccagctccg gtacaccttg tccgcccgct
     3481 cgaaaacgat tgctggttta tgcggatcgt acatgccggt catgatcatg cgccgcaggg
     3541 cgcgccggaa ggtgcgcttg aaatgccgca aagactccgg ccccgactgc cgcacgctgc
     3601 tatagcgatc cttgaccgcc gtgatgttgc gccggccctt gggttgaatg cgcggcagtt
     3661 gcagctcctc gcccagaatg tccgccaatt cgtccaaact tacatcgact tcaaggatgt
     3721 gacgcccagg atcggtgccg ccgggtccga tcccgctctc atccccatcg accgcctgcc
     3781 cgggctgccc ctcgccctgc ccgacgccgc tgtcgttgtt gtccccatag cgaaagtgcg
     3841 gcaaatcaat cccgcgcacg ggaatgctga cgaggtgttt gccctctttg ccgatcagct
     3901 cgccgcgggt aaggaaacgg cgcagatcgg cgcggatttt ccccttaaca atttggcgga
     3961 accgctgatg gtccttatgt atcttcaccg ccgcctcagg gtgatgaggt gataaggcgt
     4021 aagggcgtgc cggcgcggcc accgttatcg ccttatcacc ttttcacttt atcttttctc
     4081 agtcgcgctg cttggcatcg ccacgcgcaa aaatgctcgc cacgtagttt agtacgtccg
     4141 tggcactcgt ctcgttgtag ccgaaaaact tgatcagccg ctgcttaacc acgtcgatct
     4201 tggcctgggt ctcatcgtcg actacgcccg aaaccacgtt cttgagcttg atcgagtcgc
     4261 gcgaatcctc aaacagcttc aactccagcg ccttatgcag ccgggcgttc gtgtgatatt
     4321 cgaacttctt gccgtccagc gccaaagcgc cgatgtagtt catgatctcc tgacggaagt
     4381 cgtctttgcg gctttcggga atgtcaatct tctcttcaat cgagcgcatc agccgctcat
     4441 ccggctcctc gtcgcggccg gtgtacttgt tgcgcacccg ctcgtgctgc gtgtacgcgc
     4501 gtacgttctc aatgtagttc gtacacagcc gggcaatggc gtcctcgtcg gccgagatgg
     4561 cccgctgcac ctcggtcttg aggatgtctt cgtattcctc gcgaacgacc gccagcagct
     4621 ccttataacg tttcttcagt tcctcatcgc tgatcagcga atggtgcgac agcccgttct
     4681 cgagctcgtt catcaccatg aacgggttaa tggcccggtc ctcctgggcc tgctcgctca
     4741 ccagcgcatt ggaaatcttg tcctgaatgt aacgcgggct gatgccctgc atgccttcac
     4801 ggtgcgcctc ttcacgcaac tcgcggattg aatcctcggt aaagcccgga atgctcttgc
     4861 cgtcgtatag cttgagcttc tgcagcaggc tgaggccggc cttcttgggc tcttccagcc
     4921 gtgtcagcac cgcccacatc gccgccatct ccaccgtgtg cggggcgatg tgaatgccct
     4981 tgaccttgtc gcggccgaag tccttaccgt aaatcttgat ctcgtccgtc agccggatgt
     5041 tataaggaat atctatcttg atggtgcggt cgcggaacgc ctccatcagc tcgtttgatt
     5101 gcagtttctt gtattccggc tcgttggtgt ggccgatgat gacctcgtcg atgtcggtct
     5161 gggcgaattt cttgggcttg atggtgtgct cctgcgtcgc gccgagcagg tcgtacagga
     5221 acgccacgtc gagctttaga atttcgatga actcacaaat gccgcggttc gagatgttca
     5281 attcgccgtc aaaattgaac gcccgcgggt ccgagtccga gccgtagagg gcgatcttgc
     5341 gatagttgat gtcgccggtc agctcggtgg aatcctggtt tttctcgtcc ttgggctgga
     5401 acgtgccgat gcccacgcgg tctttctccg ccagcacaac gcgccgcacg accacgtgat
     5461 ccatcaccgc gcgccaatcg cctttatagc gaatcagcag atcttcgaac gtcttgcggc
     5521 agaacgggtc caggtcgccg tccacgcgca cctggcgatc ttccggcagg tcggcgttca
     5581 gcttggccaa aatgtcccgc cgcgcccggc gcggaatcag cttcagcggt tcctcgtgca
     5641 tcgggcacgt cgcaacctcg gcctcgttgg cgtcgccgat gcccttccac gcgaagctgt
     5701 aaagcgcccc ctcatccagc cgcgaatagt gctccaagcc cttcttcagc agacgcacga
     5761 tggtgctctt gctcgaaccc accggcccgt gcagcagcag aatgcgacgg tcggtcccgt
     5821 agccctgggc ggccgacttg aagcagtcga ccagccgcat cagcgccccg tccagcccgt
     5881 acactgcatc cgccccattg tcgaacgggt cgtcgaagaa cttgtagtgc acgtactcct
     5941 gcttcatgct cgtgtagcgc tgcgtgccgg agtacaggat catgtcgtaa atgcgctgga
     6001 acgcgttgcg cgcgatgcgc ggattggcca gcaccatgtc caggtactcc cagaacgttc
     6061 cctcccagtg ctgctggcgg aacgactcgc tgtccagatg ccggtcgacg atctccagta
     6121 actcgtcgcg cttggccatc gtacgcctcc gtgtcgtgtc ccaggctgcc gcctgcacct
     6181 gtacgatccg tccgccccgt ggattttggc tccagtcggc caccgcgggc ggctggcggc
     6241 cgcctgatgc taggaaacgg cactatttta gtcaaatgat ctgtcgccgc tttcgcccgc
     6301 cgcccccgtg cgccgcgcac gcaattaccg cctcattgtc agggttgcgc cgccggcgca
     6361 gggacaggcg ggcgagccgc cgaaccgact gacatgctgc cgcatttggg gcagcgcagg
     6421 atggtcgcgg ctgtttcccg gtcttcgcgc cccaccttac tgacgaattc tgttacgcac
     6481 tcggcgcagc gccaaacctt ccagcaatcg tgggccttgc acttcgggca cgtgagcggg
     6541 aatttgtcac gcgtgctgac ctggaccagt tgctcgtgcc cgcattcacc gcaacgcagc
     6601 ttgtcagagt aagcgcgcgg ctgcggagcc acccggtctt gcgagctgaa cgcatgatag
     6661 agccaggccg caccgccgat cagcacaagg ccaagcagta tgcccagcac gttacggcgc
     6721 atgtgccgcg cgtaggcgtt gagttcaaca tcgccccgac gctccagcat cggccggtag
     6781 ctggtcagct tttgcggacg gcggcctaag cgcacttcaa tcaatctccc gcccgattat
     6841 actcgattcg ccttgcggcg ctgcaacgcg ctggtctgcc ccgctgcggt taggtctctc
     6901 ctgggaaatg cgcgccggcc cgttctggcg ccggcttcgc cgcgctgtat cattgctcag
     6961 ctattgtcaa cttcacgagg accaagaatt gacaagcgct ctcagcttcc gaaccggcat
     7021 cggctacgac ctgcatcgcc tgactcacgg ccgcccgctc gtgctgggcg gcgttcgtat
     7081 accacatgcg cgcggcctcc gcggccacag cgatgccgac gtcgtgctgc acgccctctg
     7141 cgatgcgcta ctgggcgccg ccggcctggg agacattggc gagctgtttc cagatagcga
     7201 tccaaagtgg gccggcgcgg acagccggct gttcctggcc aacgtgctgg ctcgagtcgc
     7261 cgccgcgaaa tttcgaatcg tcaacgccga catcatcatt cacgctcaag aaccgcgcct
     7321 cggaccactg aaggcggaca ttcagcgctc cctcagcgaa ctcttgaacc tgccccccgg
     7381 cgcagtcggc gtcaaagcga cgactaacga aggcttggac gccgtcggtc gggccgaagc
     7441 catcgcctgc tgggcggccg tcttgctggc tggcacggcg tagccggcac gcaggcgcca
     7501 tcgattcgcg gcacgggacg ggctggaagg ctgggaagga cggcgggctt ggttacaatc
     7561 agcgcagaag ttgagtcgcg ccttgatcac gcagtcttcc gtttcgccgc cccctcccgc
     7621 cgtcgtggta cgcaacctcg aaaaacactt cggtgatttc gtcgccgtgc acgatgtaag
     7681 tttcgaagtg gccgcggggc agatcttcgg gctcctcggc ccgaacgggg ccggcaaatc
     7741 gaccaccatc cgcatgctct gcggcattct cacgcccacc gccggctccg gcaccgttgc
     7801 cggcttggac atgctcaccc agccggaact catcaaggcc cgcatcggct acatgagcca
     7861 gcgcttctca ctctatgaag atctgacagt cgcggagaac atcgacttct acgccggaat
     7921 ctacggtctt tctcgcgccc gccgccgcga gcgcaaggaa tgggtcatcg aaatggccgg
     7981 cttgcaggaa caccggcgct cgcggaccgc gattctctcc ggtggctgga aacaacgcct
     8041 ggccctgggt tgcgcaattc tgcacgagcc gccggtcgtg ttcctggatg aaccgaccgc
     8101 gggcgtcgac cccatcagcc gccgccgttt ctgggatttg atctatggca tggcggccgg
     8161 cggcgtaacg gtcttcgtca ccacgcacta catggacgag gcggaatatt gtgaccgcat
     8221 cggcctggtc taccgcggcg agctgatcgc caccgacacg cccgagcggc tcaaaacgca
     8281 gctccacgac gaggacgtgc tggaagtcga atgcgaccgc ccgcaggatg cgctggccat
     8341 tctcgagcag gtgcccggcg tcagggaagc ggccctgttc ggaaggggtt tacacgtggt
     8401 ggccgcgagg ggcccggcga cgatccaggc cgtacaaagc acgttggcgg cgcacgcttt
     8461 cggggtcacg cgcgtcaccc gcatcgcccc ctctctggaa gatgtcttcg tttccctcat
     8521 cgaagcccgc gaccgccagg aagccccgca agccgaggtc cgcgaatgag ttgccgcccc
     8581 acccgcgggt ttgacccgtt cagcacgctc ggctagtctg gccactttcg tattttgggt
     8641 gaaatcgtag cccgggcatg ggacagaatc gaccgaaagg cgtcaaacct cgcgatcccg
     8701 ccaaggagcc ggcggtccag cagatcgcct ccttcgtggg tttctttatc tatctcctcg
     8761 tcttaaaaag cttttttcta cctcttttcg tgatccctac gggttcgatg gcggaaacgc
     8821 tctacggcgc tcacgcgatt catacctgcc ccaactgcgg attcgagtac ccgatcggaa
     8881 tctcgccgcc ccctcatcgg gaaatcccca ttatccgctg cccgaattgc ggctggcagg
     8941 aattcaccga ctctcccaac ctgatgcagc gctatcgtac ccccttcctg cggatcgatg
     9001 gctcgattga cagtccgctg cacacgcgcg gcggcgatcg aattatggtc cacggctggg
     9061 cctacgacct gggcggccga tacgcgccgc agcgctggga cgtggtcgtc ttcaaggtgc
     9121 ccagcgatct gacaaccaac tacatcaagc gcctgatcgg catgcccggc gaacaaatcg
     9181 aaatcatcga tggcgacatt tgggtcgcac cgcccggcgg caagctggcc atcgctcaca
     9241 agccgcccca ggtccaggag gcgctctggt tctcattcta caaccatgac ttcccaccgg
     9301 cggggccctc ccaggacggg cgctaccatc cgcgttggat cacggttggc aacagcgacg
     9361 gctggcaggg cttggacacg cgctttccca gcttcaacgg cgagcccggc gagaacggac
     9421 ggatcatgtt cgtgactgag gccggggcca ctatgaaccc cggccgcatc accgacgtct
     9481 acggctatga ccgccccgcc gcccgggaca atcgtggcaa cctccggccg gtggaaccgc
     9541 acatcgtctc cgatgtgcgc ctgagttgcc gggtggattt tgattcggat gccccgccgg
     9601 aaacctttgt tgagttgagc gctacgaagg acggcgacag tttcttcggc cgcatctacg
     9661 ccgacggccg gttgatgctg gagcacgtcg gcaagagggg cgtacgggaa tcatgggggg
     9721 aggcgcgtct gaagcgcccc gggcggccgg tagtattcgc actcgccagc gtggattaca
     9781 cggtcagcct acaggtggac ggcgaggtta ccttgcaatc cacgcccgag cagtaccatg
     9841 tcgatgctgc ccaggctcgc gcgcacagcg aggccgcgat ttcgccccag ttgctgatta
     9901 ctgccggcgg agggccggtc catttggcgc atttgctcat cgaacgcgac gtctattaca
     9961 ccagcgattt gggaacgggc accgcgggct acggcacaca gcggcacccg attacgctgg
    10021 ggccgaacga ctacttcatg ctgggtgaca atagtcccta tagccaggat tccaggtatt
    10081 cgttcgtctc cagcccggac gagccgctgt tggcctcgta tgtgaatccc gactatcaac
    10141 ggggcacggt gcccggtgac cagcttattg ggccggcctt cctcgtgtac tggccaggaa
    10201 tgggacagct cctgcccgaa cgatatatga gtggggcctt gcggcatctg aatgcattgc
    10261 cgacgcccgg ccgcatccgt tggatacgct gacatcgccg aagtccatgg ccttgcaggt
    10321 ctgctattga acagcttgcc cccggaaaac agccccgcag tccggacgtc gcccgctccc
    10381 atcgggtact cgccctcgca ggccacccgt atcgtcgaga cgctacagac tgtcctgacg
    10441 gccctgctgc tcgcgttcgt cttccgggct tttttggtgg aggcgttcat cattcccacc
    10501 aaatccatgg cccactcgct gctcggggca cacgccacgc gcact
//
LOCUS       DAOSYS010000121        10346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5482_length_10346_cov_5.269459, whole genome shotgun sequence.
ACCESSION   DAOSYS010000121 DAOSYS010000000
VERSION     DAOSYS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10346
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5482_length_10346_cov_5.269459"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..928)
                     /locus_tag="PLU99_08815"
     CDS             complement(29..928)
                     /locus_tag="PLU99_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17197.1"
                     /translation="MKYCSWFVLAAMLLAGPGLARAHQTGDVNCDGAINAFDIDPFVL
                     ALTDPVAYAAEYPGCDILLADVNHDGAVNAFDIDPFVACLTGGGCEPLPIYPCELAGN
                     SLTAYPYFEFVRAFHASAPIKVALDPTRHPEITFPFTADVYVCEKKSAGEWAIDPVLD
                     DATSDGPLTVTFPGGTIQDNAFDVTGPHELNAAVYQEATGAYTGLGHGYDMIIDVNQN
                     GVLDGGDYIDGLGREAGLYVCHDTTTRGPLAVTETVYNVGTIFGIPSSVSGQDLYYPT
                     DIASMGQLPLIVISHGNGHNYQW"
     gene            complement(1180..1821)
                     /locus_tag="PLU99_08820"
     CDS             complement(1180..1821)
                     /locus_tag="PLU99_08820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOO17198.1"
                     /translation="MKRNRRPGTVKAVLQRDFYDRPVIEVARELLNKSLLCETPDGVA
                     GGLIVETEAYLAADDPACHAFRGMTRRNASMFGPPGHAYVYAIHSRWCLNIVTEPEGV
                     PSAVLIRAIQPEIGLDLMHARRTARARSLVGDAHVRPVPERDLARGPGRLCEALGIDR
                     ALDGWDLVLGRKLWIEDAPAPAGEIVTTTRIGVTAGHELPLRYFVAGNRFVSR"
     gene            complement(1923..3359)
                     /gene="cls"
                     /locus_tag="PLU99_08825"
     CDS             complement(1923..3359)
                     /gene="cls"
                     /locus_tag="PLU99_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOO17199.1"
                     /translation="MSGFLVVLLIANYMLALVVILSVLRQRKEPMAMLCWIMAVLILP
                     FLGTLLYWLIGSAWVVRRTRRRRRRVARQIAMLEAWARQQADGTGEEPDFPEDLRAIA
                     GLGRRLSDLPATRQNAVEIYQESEVTFAALEDAMRAAKHHIHAEYYIWRPDETGYFFR
                     DLLIEKARSGVECRVLLDSVGSFSIGRKFTRPFTEAGGKIAFFMPLRPFRKNISPQLR
                     NHRKIVVVDGQIGFIGSQNIGDEYRGRLKKLSPWYDTHMRVAGPAALVLQRTFCEDWL
                     FATRQDLSGQDYFPGPARPGRSIVQILPTGPEQNINPLEQIMFAAVSSAKDKIRIATP
                     YFVPSPALRMAFTYACTRGVTVELVLPTRSDALIVLWAGRSFYRELLERGVKIYEYDG
                     GVLHSKLVTVDDRWCMLGSANMDMRSFRLNYEVTALIYDQAVTRELAASIDRFCRRAR
                     VISLRELRERPRYYEVIEGTARLLTPLL"
     gene            3518..4465
                     /locus_tag="PLU99_08830"
     CDS             3518..4465
                     /locus_tag="PLU99_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO17200.1"
                     /translation="MIKDPGKAAIRISLAVSVLMLAGKLTAYFLTHSTAILGDALESV
                     VHCAATGLAALAYWYAAKPADPEHTYGHGRIAYFSAGFEGALVLAAALAVMASGIRGL
                     LYGPDLKHVGAGLAITGALALINLALGIFLIRVGRKQNAMILIANGKHVLSDVWTTAA
                     AVIGLTLVLIFRVDLLDPIAALVISVVIMTGGVSLVKGAYRGLMDKADPAVRDALIRE
                     LRERQAEGLIEQYHQLRCRGTENELWIDVHLLLPGELKLESAHTRVTLLEEALRELFP
                     DRKVYVTSHIEPATHADAHPEGHEGVPDPLAQQNPPAAI"
     gene            complement(4600..5349)
                     /locus_tag="PLU99_08835"
     CDS             complement(4600..5349)
                     /locus_tag="PLU99_08835"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOO17201.1"
                     /translation="MPQVELNELERRVLGVLIEKSLAQPSYYPMTLNAVIAACNQKSN
                     RDPVMELDEDTVWNTLDALRERGFVSRILAGMGGRVDRFRHEVKTALNWEKPQRAVMT
                     ELLLRGPQTLGELRSRCMRLYPFENAEAIAAVVQMLCETDPPLVAPLPRAPGQSAARY
                     AHCLYPPGEQPAVAAQPLASEPASRAFAAQSSADSSPPASLPDACASEPQQQSTADDS
                     RLQVIEEELSALREAITDLQKRLAALEQQLL"
     gene            6531..8468
                     /locus_tag="PLU99_08840"
     CDS             6531..8468
                     /locus_tag="PLU99_08840"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOO17202.1"
                     /translation="MSSRRRSQSCAAVGAVACVVALAGGWWVKDLDPAAVAAQDAAPV
                     DTNNTRAASLLMSSGERGSVPAVALQGVYWGDHKDYMLPLDGRPTIVVLVDPAKTLVP
                     MELIRGLRMVERFGDRLRLAVVFVGDRAKDRNVVESALEQRSLLKLGLRGLPDFLSLT
                     DRSGLFHKELGNPSPTHAILVAADGTIIGREILIGPFSEELTRIERTLDQTGAADTGM
                     ADADEKMAAISKAVQAVPLLPPDPDAARLPQRLRLVRRLDAAAVVAQMAAVDPDAALA
                     AVRGYCENGSNFSLARRLAEAAAERFGAERCAWARPLLDSRADGYVSVSHWQLGTALS
                     VDNASDLRMYVELQCTLEARWSGSRVAELTQVALLRGVSEARLESDKFETVNAGWGDY
                     TVHSTIKVIMSEGEWPNVHEFPVLTRAWTENVAFLPRNESPEEDDADVADFSPATRAL
                     RAGFNQALWAARRAGSAGEQEQGGRSVRGVLAQVEQWVTSAAGQLFRSTGLVGKPFGG
                     LKTSSWVQGKERLGLGEGGTLASPQGRVLLVDFIFVGCPPCRAALPRLSKLDAEYRER
                     GLDVVSVATSWGARGLFRLVEQDKLTHAVGVLCEKEEQRYGVQAFPSYLLIDRRGNVR
                     WSCTGKEPSRELIEQLLEEGN"
     gene            8864..9358
                     /locus_tag="PLU99_08845"
     CDS             8864..9358
                     /locus_tag="PLU99_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17203.1"
                     /translation="MSFTVANGASGFSRFGQASAEVEEVFSGSHAVLAKLIAERAHRF
                     GFEPKGQKTGYVNFSLERRAHTQIHPHPDGVALVVREAADELPDFQYLRAVPCSGLRR
                     ARGRNASWLQGDGRYTFRPAAAVLVPAELAESAVAPGWAEVDAVLSYAKSKRAADGLW
                     STRK"
     gene            9355..9849
                     /locus_tag="PLU99_08850"
     CDS             9355..9849
                     /locus_tag="PLU99_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17204.1"
                     /translation="MTEHKRSLPDDGGSQGERSILLRLSADEFRRLSYVADALQLSAS
                     ATLRALIPNIERPDRQVVSTPADIASAAGFDVVPVAEDFDARRLRRLLADLRAENAAL
                     RLCDEIEQQLCERGLRTLRVDTYKRLGRWCHPHRWTDREKRVRPLAEELSRLLFGCVI
                     ERFG"
     gene            complement(9939..>10346)
                     /locus_tag="PLU99_08855"
     CDS             complement(9939..>10346)
                     /locus_tag="PLU99_08855"
                     /inference="COORDINATES: protein motif:HMM:NF020437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1829 domain-containing protein"
                     /protein_id="HOO17205.1"
                     /translation="AVNDMFVMAQPRVAGFFWEDVREFLDRHDVRYSPRVKITGRSGF
                     DHAIDFLIPKSRRRPERLVQAINAPNKNTIGTYLFTLSDTREARGEASEAYAFLNDSA
                     REVTGDVIEALHAYDVKAAPWSRRDQFIADLAN"
BASE COUNT         1876 a         3039 c         3516 g         1915 t
ORIGIN      
        1 acgaggccat gtggaagccg atgtggtctt accactggta attatggccg ttgccgtggc
       61 tgatcacgat cagcggaagc tgccccatgc tggctatatc cgtcgggtag tagagatcct
      121 ggcccgaaac gctggatgga atgccgaaga tcgtgccgac gttgtaaacc gtttccgtca
      181 cggccagcgg cccgcgcgtc gtcgtatcat ggcatacata caagccggcc tcgcggccca
      241 gtccgtcgat gtagtcgccg ccgtcgagca cgccgttttg attcacgtct ataatcatgt
      301 catagccgtg gccgaggccg gtgtaggcgc cggtcgcttc ctggtagacg gcggcattca
      361 gctcgtgggg tccggtaaca tcgaacgcgt tgtcttgaat ggttccaccc gggaaagtca
      421 cagttagcgg accgtctgag gtggcgtcgt ccagcaccgg atcaattgcc cattcgccgg
      481 ccgacttctt ctcgcatacg tacacgtcgg cggtgaaggg gaacgtgatt tccggatgcc
      541 gggtcggatc caaggccacc ttgatcggcg cgctggcatg gaacgcccgg acgaactcga
      601 agtacggata ggccgtcagc gagttgccgg ccaactcgca cgggtaaatc gggagcggct
      661 cgcatccgcc acccgtgagg caggccacga acgggtcgat gtcgaaggcg ttgaccgcgc
      721 catcgtggtt cacatcggca agcaggatgt cgcagccggg gtattccgca gcgtacgcga
      781 ccgggtcggt cagagcgaga acgaacggat cgatatcgaa cgcgtttatc gcgccgtcgc
      841 agttgacgtc gccggtctga tgggcgcgcg ccagaccggg gccggccagc aacatggccg
      901 ccaaaacgaa ccaagagcag tacttcaaag cctgttcctc ctatgctaaa tacatgacac
      961 acttctcccc tcggccggcg caaccaagcc tagcctattc gagtccagta tatcgccgca
     1021 ggaccatcgg tccaagctaa atatcggggt ttgcgcgggg ccaactcgaa aaaaagggac
     1081 gcgctctggc cgatatgcac cttttccgga gcatcgcctc gccagggcca cgaccgcagt
     1141 cgtccgcccg tggccgcagg tcgagtcttt gcggcgcggt cagcggctca cgaagcgatt
     1201 gcccgcgaca aaatagcgca gcggcagctc atgtccggcc gtcacgccga tccgcgtggt
     1261 tgtcacgatt tcccccgccg gcgccggcgc gtcctcgatc cacaacttgc gccccagcac
     1321 caaatcccaa ccatccaggg cccggtcgat accgagcgcc tcgcacagcc ggcctgggcc
     1381 gcgcgccaag tcgcgctccg gcaccggacg cacgtgggca tcccccacga ggcttctggc
     1441 gcgtgccgtg cggcgcgcgt gcatcaagtc cagacctatt tcgggctgga tggcccggat
     1501 caggacggcg ctcggcacac cttctggctc cgtgacgatg tttagacacc agcgcgagtg
     1561 gatggcgtac acgtaggcgt gcccgggcgg cccgaacata ctggcgttac gccgggtcat
     1621 gccgcgaaac gcatggcaag ccggatcgtc ggccgccagg taagcctcgg tctcgacaat
     1681 cagaccgccg gctacaccgt cgggcgtctc gcacagtagg ctcttgttga gcagttcccg
     1741 cgcgacctca atcaccgggc gatcatagaa atcgcgctgc agcactgcct tgaccgtccc
     1801 cggccgcctg tttcgcttca tgcccgcaag tttacgtagg cacagggcga aaggccacgg
     1861 tggattgagg gcgggccggc ggtgatcgcg gccacggcct ggccagcccg agagccggct
     1921 tgttaaagga gcggcgtcag gaggcgagct gtgccttcga tgacctcgta atagcgcggg
     1981 cgctcgcgca gttcgcgcag actgattacc cgggcccggc ggcagaagcg gtcgattgag
     2041 gccgctaact cgcgcgtgac ggcctgatcg taaatcagcg cggtcacctc gtagttcagc
     2101 cggaaactgc gcatgtccat attggccgaa cccagcatgc accaacggtc gtcgacggtc
     2161 accagcttgg agtgcaacac tccgccgtcg tattcgtaga ttttcacgcc gcgctcgagc
     2221 agttcacggt agaagctgcg gccggcccag agcacgatca aggcgtcact gcgggtgggc
     2281 aagaccagct cgacggttac accgcgagtg caggcgtaag tgaacgccat gcgcagggcc
     2341 ggactaggca cgaagtaggg tgtggcaatt ctgatcttgt ccttggcgga ggagaccgcg
     2401 gcaaacatga tttgctcaag cgggttgatg ttctgctcgg ggccggtggg gagaatctgc
     2461 acgatagacc ggccggggcg ggccggtccc gggaagtagt cttggccgga gagatcctgg
     2521 cgtgtggcga acagccagtc ttcacagaag gtgcgttgca gcaccagcgc ggccggtccg
     2581 gcgacgcgca tgtgggtgtc ataccagggg ctaagctttt tgagccgccc gcggtattcg
     2641 tcgccgatgt tctggctgcc gatgaagccg atctgtccgt cgacgaccac gattttgcgg
     2701 tggttccgca gttggggact gatgttcttg cggaaggggc gcagcggcat gaaaaaagcg
     2761 atcttcccgc cggcctcggt gaaggggcgc gtgaacttgc ggccgatgct gaaagagccg
     2821 acggagtcga gcagtacgcg gcattcgact ccggagcggg ctttttctat caacaggtcg
     2881 cggaagaagt acccggtttc gtcgggccgc cagatgtagt actcggcgtg aatgtggtgt
     2941 ttggcggcgc gcatcgcgtc ttcgagggcc gcgaaggtga cctctgattc ctggtagatt
     3001 tcgacggcgt tctggcgggt ggccggcagg tcggaaagcc ggcggccaag cccggcgatg
     3061 gctcgcaggt cctcggggaa gtcaggttct tcgccggttc cgtcggcctg ctggcgggcc
     3121 caggcttcga gcatggcgat ctgacgcgcc acgcgccgcc gccgccgccg ggttcgacgt
     3181 accacccacg ccgagccaat cagccagtac agcagcgtcc cgaggaaagg caggattagg
     3241 acggccatga tccagcacaa catggccatg ggttccttac gttggcgcag cactgacaga
     3301 atgacgacca gcgccagcat gtaattagcg attagcaaga caacgaggaa acccgacata
     3361 ctgccagttt cggcggctgg tttggaggtg tcaaaccagg gcatgaggca gaggtcagca
     3421 gtgaagagcc ggtaagctct cgcgacgcgc gggagcatcg gggcgggcgc gggcgtcgcc
     3481 gccagtcgcg ggctaaaatg gcgctgggag gtggcgcgtg attaaagacc ccggaaaggc
     3541 ggcgatccgc atcagcctgg ccgtgtcggt cctgatgttg gcgggcaagc tgacggcgta
     3601 ctttctgacg cacagtaccg ccattctggg cgacgcgctg gagtcggtcg tccactgcgc
     3661 cgcgactggc ctggctgcac tcgcctactg gtacgccgcc aagccggccg atcccgagca
     3721 tacctacggg cacgggcgga tcgcttactt ttcggcgggg tttgaaggcg ctctggttct
     3781 ggccgcggcg ctggccgtca tggccagcgg catcagaggc ttattatatg gcccggattt
     3841 gaagcacgtc ggcgcgggcc tggccatcac gggcgcattg gcgctgatca acttggcgtt
     3901 ggggatattc ctaatccgcg tgggccgcaa acagaacgcg atgatactga tcgccaacgg
     3961 caagcacgtg ctgtcggacg tttggacgac ggccgcggcc gtcatcggct taacgctggt
     4021 gctaattttc cgggttgatc tgctggaccc gattgcggcg ctggtgatca gcgtggtcat
     4081 catgacgggc ggcgtctcgt tggttaaagg agcctaccgg ggactgatgg acaaggccga
     4141 cccggcagtc cgggacgcgc tcatccgcga gctgcgcgag cgccaggccg aggggctgat
     4201 cgagcagtac caccagcttc gctgccgcgg aaccgaaaac gaactgtgga ttgacgttca
     4261 tttgctactg cccggggagt tgaagctgga gtcagcgcac acgcgcgtca cgttgctcga
     4321 ggaggccttg cgcgaactgt ttcccgatcg caaagtctac gtgacgtcgc acattgagcc
     4381 ggccacgcac gcggatgcgc atccggaggg gcacgaaggg gtgccggatc cgctggcgca
     4441 acagaacccg ccggccgcca tttgagtttg ctgaaccggc cgcttgacgg tcatttgctg
     4501 gaccgaccgc ctggcggtca aaggcccggt gcggttcgtc aacggacacg gccacgtgag
     4561 tctgctcgcc atattagctt gctcgccatc tgagcttctc tagaggagct gctgttcgag
     4621 cgcggccaaa cgcttctgca agtctgtgat ggcttcgcgc aaggcagaca gctcttcctc
     4681 gatcacctgc aagcgcgaat catccgctgt tgattgctgc tggggttctg atgcgcaggc
     4741 gtccggcaat gaggcagggg gggagctatc tgccgagctt tgggcggcga acgcgcgcga
     4801 ggccggttct gacgcgagcg gctgtgccgc cacggccggc tgctcgccgg gcgggtacag
     4861 gcaatgggcg tagcgcgccg cggactggcc cggcgcccgc ggcagcggcg ctaccagcgg
     4921 cggatccgtt tcgcagagca tctgcaccac cgcggcgatg gcctcggcgt tttcaaacgg
     4981 gtagaggcgc atgcagcgtg accgcagctc gcccagtgtc tgtgggccgc gcagcagcag
     5041 ttcggtcatg acggcccgct gcggcttttc ccagttgagc gcggtcttga cctcgtgccg
     5101 gaagcggtcc acgcgcccgc ccatgccggc cagaatgcgc gagacgaagc cccgctcgcg
     5161 cagggcgtcg agcgtgttcc agacggtatc ctcgtccagt tccatgacgg gatcgcggtt
     5221 ggacttctga ttgcacgcgg caatgacggc gttcagcgtc atcgggtaat acgacggctg
     5281 ggccagcgat ttctcaatca ggactcccag cacgcggcgt tcgagttcgt tcaattctac
     5341 ctggggcacg tcatcctcct gacgagcgcc gccgacagcg gcgcggcgag tacgggacaa
     5401 gccaacttac tggcgaactg tggcgcgctc aagaaggcgg gggaagctgt cagccttcag
     5461 ccgtcagctt tcagccaagg caactccgct cgtgcgagaa tccgtctcgc acgccctccg
     5521 gcgggaatcc tttgccgcac cctggcccgc cctcaccatt ccggcagatc gacttcaatg
     5581 cagctcgttc cctcgccggc atacgccgcg gctgagctga accgccaatg ttccgggcgc
     5641 tggaccagcc gcgcgagcgg cgggggcgcg aggcgttacc ccaggtgcgt aagcggtctg
     5701 ataaaaagcg gcgtgagatc gggccatggg ggcaggtgat cagatacgca tggttcttgt
     5761 gcactcgatt ttgataacct gaggcccgcc gctgcgcgga gagaggtgat aggtgagagg
     5821 cgagcaggtg acaaaagcgg ttgcgcggag acagttgata ggtggcaagt gagcattgag
     5881 gacgtttcga atgccgcata agggcgcctt tctgcgcgcg gggcgcggtg tcttctatcg
     5941 attctagaga gcggcccgcg ttcagttgtc acgtttcggc caatagtccg cggattgtgc
     6001 gtggttgcgc gccagtggcg gcgagacgct gcttggggtg ataaagtgat aaggtgatag
     6061 ggtgataaag aaagctttca gccatcaccc atcggctttc agctaaggca accggcaaga
     6121 cgctgagagc gccaagaccg ccatgaagga aaagctgcgg cgcgtggggc agaggatttg
     6181 cgcggcccgc tcgtgcgaga atccctctcg cacgccctcc ggcgggaatc ctttgccgcg
     6241 ccgcagctct tagagctcaa cgaacaacta ggcgcgtcgc tgcgtccccg ttctaccagg
     6301 cgttctgcca gtagctcctc cccgttctgc caatgcagcg caatccgaga tctgaaatct
     6361 gaaaatcccg gctgggtgcc gttgcggtga gtggaactga atttcgtagt agtctttggg
     6421 tagactgccg tgggttgcag cacggttgta tcgggcggac cgcagaccgc tttcgcggca
     6481 agtgggcagc ctcggggtgg cgctgcggca cccaacgcag gagagtgaaa atgtccagtc
     6541 gacggcggtc ccagagttgc gccgcggtcg gtgccgtggc atgcgtcgtg gcactggcgg
     6601 gcgggtggtg ggttaaagac ctcgacccgg cggccgtcgc ggcgcaggat gctgcgccgg
     6661 tagacaccaa taatacgcgc gcggcatcgc tgctcatgag cagtggcgaa cgtggcagcg
     6721 taccggccgt ggcgctgcag ggcgtgtatt ggggggacca caaagactac atgttgccgc
     6781 tcgacgggcg tccgacgatt gtggtgctcg tagatcccgc caagacgctg gtgccgatgg
     6841 agttgattcg cgggttgcga atggtcgaga gatttggcga ccgcctgcgt ttggcggtgg
     6901 tgttcgtcgg cgatcgggct aaagatcgaa acgtggtgga atctgccctt gaacagcgca
     6961 gcctattgaa gctggggctt aggggcttgc cggatttcct ttcattgact gatcggagcg
     7021 ggctattcca caaggagttg ggaaacccgt caccaacgca cgcgattctc gtagccgccg
     7081 acgggacgat catcgggcgc gaaatcctga tcgggccttt cagcgaggaa ctgacgcgta
     7141 tcgaacgcac gctggatcaa acgggcgcgg ctgataccgg gatggcggat gcagatgaaa
     7201 agatggcggc gatttcgaag gcggtgcagg cggtgccgct tcttccgccg gacccggacg
     7261 ccgcgcgcct gccgcagcgc ctccgcctgg tgcgacgcct ggacgctgcg gcggtcgtgg
     7321 cgcagatggc ggccgttgac cccgacgcgg cgctcgcggc cgtgcgcggg tactgcgaaa
     7381 acgggtccaa cttctcgctc gcgcggcgcc tggcggaggc cgcggcggaa cggttcggcg
     7441 cggagcgctg cgcctgggcg cggccgctgc tggatagccg ggcggacggt tacgtatcgg
     7501 tgagtcactg gcagctcggg actgctttga gcgtggataa tgcgtccgat ttgcgcatgt
     7561 acgttgaact gcaatgcacg cttgaagcgc gctggagcgg atcgcgggtg gccgagttga
     7621 ctcaggtggc gctgctgcgg ggagtgtcgg aggcgcgcct ggagagcgac aaattcgaga
     7681 cggtgaacgc cggctggggc gactatacgg ttcatagcac gatcaaggtg atcatgtcag
     7741 agggcgagtg gccaaacgtg cacgaattcc cggtgctgac gcgcgcctgg accgagaacg
     7801 tggcctttct gccgcgtaac gagtcgcccg aagaggacga cgccgacgtg gccgactttt
     7861 cgccggccac cagggcgctg cgcgcggggt tcaatcaagc gttgtgggcg gcgcggcgtg
     7921 ctggttcggc gggtgaacaa gagcagggcg ggcgcagtgt gcgcggggtg ctcgcgcagg
     7981 ttgagcaatg ggtgacgagc gcggcggggc agctattcag atcaaccggg ctggtgggta
     8041 agccgttcgg cggtctgaag acgtcatcct gggtgcaggg aaaggagcgc ctggggctag
     8101 gggaaggggg tacgctggcg tcaccccagg gacgcgtgct gctggtggac tttatattcg
     8161 tggggtgccc gccgtgtcgg gcggcgctgc caaggttgag caagctcgac gcggagtacc
     8221 gcgagcgcgg cctggacgta gtttcagtcg cgacgtcgtg gggcgcgcgc ggcctttttc
     8281 gcctcgtcga acaggacaag ctgacgcatg cggtcggtgt attgtgcgag aaggaggagc
     8341 agcggtacgg ggtgcaggcg tttccgagtt accttttgat tgatcggcgc ggaaacgtcc
     8401 gttggtcatg cacagggaaa gagcccagcc gggagctgat cgagcagttg ctggaggaag
     8461 ggaattaggc gaatcaggta attagactgt tgggcaatta ggcattaagg cgccgctcgc
     8521 gcgagaatcc gtctcgcacg cgctccggcg ggaatccctt gccgcgccgc agctctcaga
     8581 gctcagccat cagcgcagaa cgcgcagatt gtgagcgctt ttgcgccttt ttgtctcctc
     8641 agatgtcgca cattttcaca gactcgatcg gcctgcggcc agccgcacgc ttgcgttccg
     8701 gccgcacgtc cgcgggcagc ccgcaccaat cactagaccc gtgccgtccc cgttcgccct
     8761 ccgtcctcgt tctacccccg gctgcaaaaa ggcttgactg cggccgcgag gcatgatata
     8821 gtggtagccg ttacatatta atacatatca aaaggcggag agaatgtcgt ttacggtagc
     8881 aaatggggcg agcggctttt cgcgtttcgg ccaggcgagc gccgaggtcg aggaggtgtt
     8941 tagtggctcc cacgcggttc tggccaaact gatcgccgag cgagctcacc ggttcggatt
     9001 tgaaccgaaa ggccagaaaa ccggatacgt aaatttctct ctcgagcgtc gggcgcacac
     9061 ccagattcat cctcatcctg atggcgtggc cctcgtcgtt cgcgaagccg ccgacgaact
     9121 gccagatttc caatatctgc gcgccgtgcc ctgctccggc ctaagaaggg ctcgtgggcg
     9181 caatgcgtcg tggcttcagg gagacgggag atatacgttc agaccggccg ccgcggtgct
     9241 cgttccggca gagcttgctg agagcgccgt cgctccaggc tgggcggagg tcgatgccgt
     9301 gctttcgtac gcgaagagca agagagcggc cgatggattg tggagtacaa ggaaatgacc
     9361 gaacataagc gttctttgcc tgacgatggc ggatcccaag gcgagcgttc aattctgctg
     9421 cgcttgtcag cagatgagtt ccggcggctg agctatgtag ctgatgcatt gcagctgtcg
     9481 gcctccgcga cattgcgggc cctgattccg aacatcgaac ggccggatcg acaggtagta
     9541 agcacgccag cggacatcgc gtcagcagcg gggtttgatg tggttcctgt cgcggaggac
     9601 tttgatgcaa ggcgccttcg tcgtcttctg gctgacttac gcgcggagaa cgcagcgttg
     9661 aggctttgcg atgagatcga gcagcagctt tgcgagcgcg ggctgcgtac gctgcgagtg
     9721 gacacgtaca agcggctcgg acgctggtgt cacccccatc gttggacgga ccgtgaaaaa
     9781 cgggtccggc cgctcgccga ggaattgagc cgtttattgt tcggctgcgt catcgaacgc
     9841 tttggctgag ctgccaagca ctgcacggca tcgacttcga ggaagacgct cgcaaagggg
     9901 cacggagaat cggcgatatg ggtgctcgca cgtacgcact aatttgccag gtcggcgatg
     9961 aactggtcgc ggcgtgacca cggtgctgct ttgacgtcat atgcgtgaag ggcttcgatc
    10021 acgtcgccgg tcacttcgcg cgccgagtca ttcaagaacg cataagcctc agaagcttcg
    10081 ccgcgcgcct cgcgcgtgtc gctcagagtg aacaagtagg ttccaattgt gttcttgttc
    10141 ggcgcgttga tggcctggac caagcgctcc gggcgacggc gcgatttcgg gatgagaaag
    10201 tcgatcgcgt ggtcgaaacc gctgcgacct gtgatcttca cgcgcgggct atagcgaaca
    10261 tcgtgccggt ccaggaactc gcgcacgtct tcccagaaga agccggccac gcgcggctgg
    10321 gccatgacga acatgtcgtt caccgc
//
LOCUS       DAOSYS010000122        10301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5516_length_10301_cov_5.233164, whole genome shotgun sequence.
ACCESSION   DAOSYS010000122 DAOSYS010000000
VERSION     DAOSYS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10301
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_10301_cov_5.233164"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLU99_08860"
     CDS             complement(<1..250)
                     /locus_tag="PLU99_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOO17206.1"
                     /translation="MPREPLKIPFRVDGLSILNFNGEVDQALEPALPPDDLRRLYRTM
                     LASRRFDERCLQLQRQGRMGTYGPSKGQEAASLGAAYVL"
     gene            complement(629..793)
                     /locus_tag="PLU99_08865"
     CDS             complement(629..793)
                     /locus_tag="PLU99_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17207.1"
                     /translation="MTPLTYSHQQQFVPSERRQTMTELFLLVAAIITCSVLSVLAYLL
                     VQWFWFSNWR"
     gene            complement(956..1588)
                     /locus_tag="PLU99_08870"
     CDS             complement(956..1588)
                     /locus_tag="PLU99_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO17208.1"
                     /translation="MTHRGTVHIVDDDPSIQRSLRRLLESVGLNSECYSSAQDFIERR
                     NADAPGCLLLDVRMPGLSGLELQDHLRSEACMLPIIFVTGCGDVPMTARAMKAGAIDF
                     LQKPFNEQELLEAIERALTRNCQMRQEQAERRKVQMRLRLLTPREQEVLERVVAGKTN
                     KEIAAEFGITEKTIKVHRARVMEKMQADSLAQLVVLAQVAGVSSPNGLPV"
     gene            complement(1585..2832)
                     /locus_tag="PLU99_08875"
     CDS             complement(1585..2832)
                     /locus_tag="PLU99_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOO17209.1"
                     /translation="MSACKSATFPGEADRSVADRDLQLLREVVAVSNQVEDFEHALHL
                     ALPGIAQRYSCAVGQAFLIPDDPPARLLLCGACYESTPGLLAKVRTPDALDLENAGYL
                     RPIIDRGEPHCTTALEDALGVRGPAAREAGLAWVLGVPVIAGMKTVALLEFFAVQPIE
                     PECGVLDAVAPLGVALGRVLERQRTRSATRRNERLASVGTFAAGIAHELNNPLTSMLM
                     NARLALKHDYPKERLREILDEIAADTERCAKIVKNLVKFARQEPAERSPVNVSELIRR
                     VVAVARRAAERPSVLFEVDILEPLPVVMAEPRAIEQALVSVVSGVLHDGDSRRTVRLQ
                     AQETGGKLRICVSAGPSRDLATNQAGATETANDSAGSAYEMAGGIIAEHGGAITIDRR
                     PGGGNRITIELPVAGQSPGRATE"
     gene            3062..3841
                     /locus_tag="PLU99_08880"
     CDS             3062..3841
                     /locus_tag="PLU99_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOO17210.1"
                     /translation="MAKQAEIRIGTSGYQYDHWRGIFYPEKMAKKDWFTFFAAAFDTV
                     EINNTFYHLPQAATFDAWRAQAPHGFCFALKYSRFGSHLKHLKDPASHLRPFLERAER
                     LKPHLRPILVQLPPQWGVAADRLEEFLAAAPHRRRWAIEFRHPSWLCDTIYRILRKYG
                     AALCIHDKFEHPRLLTAGWTYLRYHGGPEEGGGYSATFLQSQAAQIREYQAAGVDVFA
                     YFNNDWQGHALRNAAALRRMLEDRAKPRRRRASEGGAKPKR"
     gene            complement(3980..4243)
                     /locus_tag="PLU99_08885"
     CDS             complement(3980..4243)
                     /locus_tag="PLU99_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17211.1"
                     /translation="MPTITLPAAMRLKRLFERAQLEENDSVRLRATTHGLEMFFDSPR
                     PGDDRVVYRNRTLFLLDPPTARRLAGRRIDLEVTEAGEALVVA"
     gene            4553..5113
                     /locus_tag="PLU99_08890"
     CDS             4553..5113
                     /locus_tag="PLU99_08890"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOO17212.1"
                     /translation="MSQTAQLHRGMVIRHEGHLYSIIDFQVVQAGKQRPTVHVKLRSL
                     KDGKQAERTLDQLGTLDEVPSEIREMQYLYASGRERVFMDLTSYDQFNVSDDLLGHGR
                     DFLVEEQSYRFLTVEGQPMALQLPPSLPMEIVDTAPPSHGGGSSSVMKEARLASGRTI
                     MVPLFIKVGDKVRVSTVDGTYQGKEH"
     gene            5238..6146
                     /locus_tag="PLU99_08895"
     CDS             5238..6146
                     /locus_tag="PLU99_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17213.1"
                     /translation="MRIWSTLNGWPWYVRGPIKLAAFGLVLLLTLYPKFWLLPRHIQR
                     LRDMDSALEPELPGLSVMAHEVWLISRPDEAVLDTVEDVVYRHIPYAWDWDTWGVVDY
                     LPSTSEVLAVGREDCDGRAVVAASLLRRMGYEAHLVCDLKHVWVSCPQGQLMSPGAGE
                     TSLVATESGTQVKFSRGLLANVVRGMTFGIAVFPLGRELIIVVALCAVLIQPRSSVFR
                     RIAGCAAMLLTLVLFRQAGDSVDGLAKHPELVVLGALTWVAACLLLAIRSGNPRAKAD
                     EARTGAAAPLSPPARGDGSTPLRSAP"
     gene            complement(6355..7446)
                     /locus_tag="PLU99_08900"
     CDS             complement(6355..7446)
                     /locus_tag="PLU99_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOO17214.1"
                     /translation="MQTARQNVLVGAFVLVGLAALGALAILFGSGLTRLLGGDGYPII
                     IEFGSVRGIRPGNPITLAGIEIGQVEEVKFAGPVQLATLGGPVVEVHAIISKEFENRI
                     PKNSTAIATEPAFGQGRPPIEITPGDPKTGMLAAGDRILDAQTKGVIESLLPQDIRFT
                     FEKATAQVGEAAEALTPVLTDMHDLLARRSPSEVDRIGGPQGNLASAVARIDAAAKHL
                     NEVLGDPAVKSQLRETIANAHKMSEDGKVFTANLRASSEKVDEVVERVQTVAAKAETT
                     VDRLDSEVTTLARDARGFLERATRLAETANEMAAAIQQGEGTLGRLLRDTKLYESMVL
                     TFQRVAQAMEEFRLMVVDWRDKGKIRVAF"
     gene            7589..7936
                     /locus_tag="PLU99_08905"
     CDS             7589..7936
                     /locus_tag="PLU99_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007324235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17215.1"
                     /translation="MDDDPDIVSAITTSLADTGATIDTARDGAKAVQLAEENDPDLII
                     LDIMLPQKSGFLVLERLRKDKPRGGRPHVIMITGNEGRRHRQYAEALGVDEYLNKPFR
                     MERLMEVTEKLLG"
     gene            8459..9325
                     /locus_tag="PLU99_08910"
     CDS             8459..9325
                     /locus_tag="PLU99_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17216.1"
                     /translation="MLYLCMLVALQQPAPSTPSGVTVVPPAPQADTQPAEPAVTYDQG
                     PLRAEILAMFDARYFFLEKTAGTRESELRLQMRFAGAGIENTVRVGNIIFTEATDDTG
                     RALVTPETYTEEQKTEMRPFNLPAERLKGSGLTLQAKLGSPARAAQTMRLRGSLRIVT
                     APEKLEISIENPLQYAGKTIPNEQLQAAGVEVRVVAAEELPEEQSQPNQLVLQIVKGS
                     EKVQEVALYDAYMKQMRPRERTLETKSGDSVKGYSITSGTLDENTQLVLRVFPRTDEI
                     TIPIELDSVPLP"
     gene            9492..>10301
                     /locus_tag="PLU99_08915"
     CDS             9492..>10301
                     /locus_tag="PLU99_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17217.1"
                     /translation="MFVRTRSLKQNALYAVTGMSAFYACQLAVIVILAKFAPSFVLGQ
                     VEFSLAVATPVVLFCSLELRGAFVADAAHEFAYGTYRMLRNLMMGVAAVVLLGFAVWE
                     AATQQNWSYTIILIGMCGSRIMLSQAEMAWGLFQRRERLDLMAASAAQRGAAMLVSYA
                     LFVPLFAYLARRGLISDGHGADGAALAIVVYVAASAAILVWFDHRQVAARHDFDPSWT
                     WTAVWKLARRTLPLGIVLLLLHLSSSVPVYVIEHQPGGIAALGHYGALEKLT"
BASE COUNT         1919 a         3155 c         3258 g         1969 t
ORIGIN      
        1 gcagaacgta agccgcgccc agcgaagccg cctcctgacc cttggagggt ccatacgttc
       61 ccatgcggcc ctgtcgctgg agctgcaggc agcgttcgtc gaagcgccgg ctggccagca
      121 tggtccggta aaggcggcgc aggtcgtccg gcggaagagc cggctcgagg gcctggtcga
      181 cttcgccgtt gaaattcaga attgaaagcc cgtcaacacg aaaagggatt ttcagcggct
      241 cacgcggcac ggtgactcct cggctcgcag atccccgccc gaacgcgggt taatctaacc
      301 aaatatttta ttctagttag ctgttctgat gatgtacaac tcacgagatg ttcgcgaaca
      361 gcgcttgaaa tcgatggtct tggtcggata tatcgaagcg cggaacaaaa accccggccc
      421 cttcgggtca agaaggggcc ggggacctcg gccgccccag tcgtcagaca cttatctcta
      481 ccagccgcca gcatcagcgc cggcgggccg gtgagcagtc aagtccggct tgcgagcggt
      541 ctcgacggat ctgtgccggt cgtgacccgc gattcgcact ccaagccggt tcgcaaacaa
      601 agccacgggc agcagtcatc cggcgacctc agcgccagtt tgagaaccag aaccactgta
      661 ctagcaggta ggccagaaca ctgagcacgg aacaagtgat aatagccgcg actaacagaa
      721 agagctcagt catggtctgg cggcgttcgc tcggcacaaa ctgctgctgg tgactgtatg
      781 tcaacggcgt cattacagct cgacccacac ctattcttcg gcgtcaagcg gcccggccgg
      841 atttcatgtc gtccggccct cgccgggacc aggttacggt tgtgtccgct caacgccgcc
      901 catgtattgg catagtgtgg cacgaaaaac gcgctggcga aattgtgtgt tgtacctaga
      961 cgggtaggcc gttaggcgaa ctaacgcctg caacttgtgc cagcacaacg agttgcgcga
     1021 gtgagtcggc ctgcattttt tccatgaccc gcgcgcggtg caccttaatc gttttttcag
     1081 taattccaaa ctccgccgca atctccttgt tggtcttgcc ggccacgacg cgctcgagga
     1141 cctcctgctc acgcggcgta agcaatcgca agcgcatctg gaccttgcgc cgctcggctt
     1201 gttcctgccg catctggcag ttgcgggtca aggcccgttc gattgcttcc agcaactcct
     1261 gctcgttgaa gggcttctga aggaaatcga tggcgccggc cttcatagca cgggccgtca
     1321 tgggcacgtc gccgcagccc gttacgaaga tgatgggcaa catgcaggct tctgaccgca
     1381 gatggtcctg aagttccagg ccgctcagtc ccggcatgcg cacgtcgagc aacaagcaac
     1441 ccggagcgtc agcgttgcgg cgctcgatga agtcctgggc ggaactgtaa cactcggaat
     1501 tcaaaccgac ggattccagc agccgccgca aactgcgctg aatcgagggg tcgtcgtcga
     1561 cgatgtgtac cgtgccgcgg tgtgtcattc ggtcgctcgt ccgggcgatt gcccagcaac
     1621 gggtagttca attgtaatcc tattgccgcc cccaggccgc ctgtcaatag taattgcgcc
     1681 gccgtgctca gcgataatac cgccggccat ctcgtaggcg gagccggcgc tgtcgttagc
     1741 agtttccgtc gcgccagcct gattcgtcgc gaggtcccgc gaagggcctg cagatacgca
     1801 gatgcgcagc ttgccgccgg tttcctgagc ctggagccgt acggtccggc gactgtcgcc
     1861 gtcgtgcagc acgccactga cgacgcttac cagcgcctgc tcgatcgctc taggctctgc
     1921 catgacgacc ggtagcggtt ccagtatgtc gacctcgaag agcacagagg gtctctcggc
     1981 cgcgcgccgc gcaacagcca ccacgcgccg aatcagctca gaaacattaa ccggggaacg
     2041 ctcggccggt tcctgacggg cgaacttgac caggtttttg acgattttgg cgcagcgctc
     2101 ggtgtcggcc gcgatttcgt cgaggatttc gcgcaagcgt tctttggggt agtcgtgttt
     2161 cagggcgagg cgagcgttca tcagcattga agtcagggga ttgttcagtt cgtgagcgat
     2221 tccggcggcg aacgtgccca cggacgccaa tcgctcattg cgccgcgtcg cgctccgggt
     2281 gcgctgacgc tcaagcacac gtccgagcgc aacgccaaga ggcgcgactg cgtccaggac
     2341 gccgcactca ggctcaattg gctgaaccgc gaagaactcc aatagcgcca ctgtcttcat
     2401 gccggcaatt accggaaccc ccagcaccca tgctaatcct gcctcacgcg cggccgggcc
     2461 gcggacgcca agagcgtctt cgagcgcggt agtgcagtgc ggctcgccgc ggtcaatgat
     2521 cgggcgcaag tagccggcgt tttcgaggtc gagcgcatcc ggggtgcgca ccttcgcgag
     2581 aagcccagga gtggattcat agcaggcgcc gcacaaaagc aaacgtgcag gggggtcgtc
     2641 cgggatgagg aaggcttgcc ccacggcgca ggagtaacgc tgtgcaatac ccggcagcgc
     2701 cagatggagc gcgtgctcga agtcctccac ctgattgctg acggcaacga cttcgcgcag
     2761 tagctgaagg tcccggtctg caacggacct gtcagcctcg cctggaaacg ttgctgactt
     2821 gcacgccgac attgtttcct cgagctcctt gccgtacgga acttcggcgg accgcccggc
     2881 gacatgggcc cactgtccgt aagctcgatg atacagcgtg ctggtgttct tacaacactt
     2941 cgttttgctg cggcgttaaa ttacagaatt gtaacacaga gccaagttct cagtgctgat
     3001 tgcgtgatac gctcacacgg acgcccgcag cagttgacgc ggttcgtctt gggctggaat
     3061 catggcgaag caagccgaaa ttcgaatcgg cacgtcgggc tatcaatacg accactggcg
     3121 cggcatcttt taccctgaga agatggcgaa gaaggactgg tttactttct ttgccgcggc
     3181 cttcgatacc gtcgaaatca acaacacctt ctaccacctg cctcaggctg ctacgttcga
     3241 cgcttggcgc gcacaagcgc cgcacgggtt ctgcttcgcg ctgaaataca gccgcttcgg
     3301 ctctcacctc aagcatttga aggacccggc cagtcacctg cggccatttc tggaacgcgc
     3361 cgagcggctc aaaccgcacc tccggccgat tctggtgcag ttgccgccgc agtggggcgt
     3421 cgctgccgat cggctggaag agtttctggc tgcagcgccg caccggcggc gctgggcgat
     3481 tgaattcagg cacccgagtt ggctatgtga cactatctat cgcattttgc ggaaatacgg
     3541 cgccgcgctg tgcattcacg acaagtttga gcacccacgg ctactgacgg ccggctggac
     3601 gtatttgcgc tatcatggcg ggccagagga gggcggcggc tacagcgcga ccttcctgca
     3661 atcgcaggcc gcccagattc gcgaatacca ggcggccggc gtagacgttt tcgcctactt
     3721 caacaatgac tggcagggcc acgcgctgcg aaatgccgcc gctctgcgcc gaatgttgga
     3781 ggaccgcgcc aagccgcggc ggcgccgcgc gtcagaaggt ggggccaagc ccaaacgttg
     3841 aggcgggtca attcagtctc cccagggtct gtctcgcact cccgcagccg tctcgatttc
     3901 agcggcacta tctctggcag caccgcgacc gcgtcaccgg cacactcaac cggccgccgg
     3961 tgtcagggca cggaaagacc tacgctacaa ccagggcttc gccggcttcg gtcacttcca
     4021 ggtcaatacg ccgtccggcc aggcggcgtg cggtgggcgg atcaagcaag aacagtgtcc
     4081 ggtttcggta cacaacgcga tcatcgccgg gccgcgggct atcgaagaac atttccaggc
     4141 catgcgttgt tgcgcgcagc cgcacggaat cgttctcttc cagttgggct ctttcgaaca
     4201 aacgtttgag ccgcattgct gcgggcaacg ttatggtggg catttccagg ctcctcattt
     4261 ctccctgatg gcgaaggcgg ccgccggtcg gtcaccacgc acatatctgt gtatcgtgcg
     4321 caactgaatc aattctgaaa tcgagattac agggagttaa ggcacaaatt aggcggctgt
     4381 aatgtgtttt tcgggggcgc ggcgttcgta atacgtcatg aacatggagc acgcacctgg
     4441 tcgccaggtt ttccatcatc ccgcccaggc gaggtgccgc gccgcagaaa agctggacat
     4501 tttgcggctc gtgcgagaca ctaaactcgg gtgtgttacg ggagaagcgg ctatgtcgca
     4561 gacggcgcaa ctccacaggg gcatggtgat ccggcacgag gggcacctgt acagcatcat
     4621 cgacttccag gtggtccagg ccggcaagca gagaccgacg gtacacgtaa agctgcgctc
     4681 gctcaaagac ggtaagcagg ccgaacgcac gctggaccag cttggcacgc tggacgaggt
     4741 acccagcgaa atccgggaga tgcagtactt atacgcgtcc ggccgcgagc gcgtcttcat
     4801 ggacctgacc agctacgacc agttcaacgt gagcgacgac ttgctggggc atgggcgcga
     4861 tttcctggtg gaggagcagt cgtaccgctt ccttacagtc gaggggcagc cgatggcgct
     4921 gcaactgcca ccgtcgctgc cgatggagat cgtcgacacg gccccgccgt ctcacggcgg
     4981 cgggagcagc agcgtgatga aggaagcccg cctggcgagc gggcgcacga tcatggtgcc
     5041 gctatttatc aaggtcggcg acaaggtgcg tgtcagcacg gtggatggga cgtaccaggg
     5101 gaaggagcat tgaagaggac gccccccggc tggtgagttg ataaggtgat aaagtgataa
     5161 agtgatcaag gcggcctggg cccaggcgct gaagcgctcg tgcgcctttg ctcagccttc
     5221 cgaattcgga ggtttgaatg cggatttgga gcacgctcaa tggctggccg tggtacgtgc
     5281 gcgggccgat taagctggcc gctttcggtc tggtcctgct tctcacgctc tatccgaagt
     5341 tctggctgct gccgcgacac attcagcgac tgcgggatat ggatagcgcg cttgagccgg
     5401 agctgccggg gctttccgtc atggcgcacg aggtttggct gatatcgcgg ccggacgagg
     5461 cggtgctcga tacggttgag gacgtcgtat accggcatat tccctatgcg tgggattggg
     5521 atacgtgggg ggtggtcgat tacttgccga gcacttccga ggtgctggcg gtggggcgcg
     5581 aggattgcga cgggcgggcg gtggtcgcgg cgtcactgct gcggcggatg gggtatgagg
     5641 cacatctggt ctgcgatctg aagcacgtgt gggtgtcgtg cccgcagggg cagctcatgt
     5701 cgccgggggc gggcgagact tccctcgtgg cgaccgaatc tggcacgcag gtgaagtttt
     5761 cacgggggct gctggcgaac gtggtgcgcg gaatgacgtt cgggatcgca gtctttccgc
     5821 tcggacgaga gctgatcatc gtggtggcgt tatgtgcggt tcttatccag ccgcgatcgt
     5881 cggtgttcag aaggattgcc gggtgcgcgg cgatgttgct gacgctggta ctgttccggc
     5941 aggcggggga ctcggttgac gggctggcta agcatcccga actagtggtt ctgggcgcac
     6001 tgacgtgggt ggcagcgtgc ttgctcctgg cgatcaggag cggaaacccg cgggcgaagg
     6061 cggatgaggc gcggaccgga gcggcggcgc ccctcagccc gccagcgagg ggcgatggat
     6121 ccacgccctt acgatccgcc ccctaaccgc cagaaagggt caccggatcc ggctgatttt
     6181 ccaaatcgcc gcgattttcc gtctacggaa aaaatggcct actgggaaaa tctcttgaga
     6241 ttcggcttcc tttccggctt cgatccgttt tgaggcggca cgccctgcgc tctttacccc
     6301 gcactgcttg aacctgcgct ttcaagcatc ggcgcacgcg ggcgcgcaac tagactagaa
     6361 tgctacgcga atctttccct tgtcgcgcca gtccaccacc attagccgga attcctccat
     6421 cgcctgcgca acccgttgga atgtcagcac cattgactcg tagagcttgg tatcgcgcag
     6481 cagacgcccc agcgtgccct cgccctgctg aatggccgcc gccatttcgt tggccgtttc
     6541 cgccaagcgc gtcgcccgct cgaggaagcc gcgcgcgtca cgcgccagcg tggtcacctc
     6601 ggagtccagg cggtcgaccg tcgtctccgc tttcgcggcc acggtttgca cccgctctac
     6661 cacctcatcc actttttccg agctggcgcg caggttggct gtgaagacct tgccgtcctc
     6721 gctcatcttg tgggcgttcg cgatcgtctc gcgaagctgg ctctttaccg ccggatcgcc
     6781 gagcacctcg ttcagatgct tggccgcggc gtcgatgcgg gccaccgcgg aggcgaggtt
     6841 gccctgcggg ccgccgatgc gatccacctc gctcgggctg cgtcgcgcga gcaggtcgtg
     6901 catgtcagtc agaaccggcg taagtgcttc ggcggcctct ccgacctgcg cggtcgcttt
     6961 ttcgaacgtg aaccggatat cttgcggcag cagcgactcg atcaccccct tggtctgggc
     7021 gtccaggatg cggtctcccg cagccaacat gccagtcttc ggatcaccgg gcgtgatctc
     7081 gatcggcggc cggccctgcc caaacgccgg ttcagtcgca atcgcggtgc tgtttttggg
     7141 aatgcggttc tcaaactcct tgctgatgat cgcgtgaacc tcaaccaccg gcccgccaag
     7201 cgtggcgagc tggaccggcc ccgcgaattt gacttcctcg acctgcccaa tctcaattcc
     7261 ggccagcgta atcgggtttc ccggccgaat tcccctgacc gacccgaatt cgatgatgat
     7321 gggatatccg tcgcctccga gtagccgcgt aagcccgctg ccgaacagaa tcgccagcgc
     7381 acccagcgcc gcgagcccta ccaggacgaa cgctccgacc aggacatttt gacgtgcggt
     7441 ttgcatgacg ctctcccaga cccagccggc cgtcgccgcg gccgatgagt ttgttgtcag
     7501 gtccagccgg gaatattaca ctacgcgcac ggccgagcat aaggagacga gagtgccgaa
     7561 atcactcgaa ggcagacgga tactcgtcgt ggacgacgat ccggacattg tgagcgcgat
     7621 aaccaccagt ctggccgata ccggcgcaac gatcgacacg gcgcgcgacg gcgccaaggc
     7681 cgtgcagctt gctgaggaaa acgaccccga tctgatcatc ctcgacatca tgcttccgca
     7741 aaagagcggg ttcctggtgc tcgagcggct ccgcaaggac aagccgcgcg gcggccggcc
     7801 gcacgtgatc atgatcacgg gcaacgaagg gcgtcgtcac cgccagtacg cggaggccct
     7861 gggcgtcgac gaatacctga acaaaccttt ccgcatggaa cgcctgatgg aggtcaccga
     7921 gaaactactc ggttagaccg gttcgacggg ccgctcgcac gccgcgcgac agagagtggc
     7981 tccgattcga attccggcat gcgcgctatg cgccctttat ctgctcggct ttcggggggc
     8041 atgcatggga cgcagcgccg gagacagccc gacaggcgga gacagcgcga caggccgggt
     8101 gccatgccca agccccgcga cagcggggcg cgggcatgcc ggccccagtt gctctatgct
     8161 gccaccagtc gctccatgcg tcgccagtcg cgccgcatcg gagaccgtta acgaggggca
     8221 tgcccgcgct tacagcttgg gcatggcacc ggcgcttaca gcttgggcat ggcacctcat
     8281 ggcacccacg cttagtcacc ggcgctgatc accaggttga cgagtagcgc ggtgggagtg
     8341 attatgttcc cgcccgttag agatcgcgct tgtgccgcgc gcggaaagtg ggaaagcggc
     8401 cagcggtcgg tcatcgccag aggaagttga actaggttga actaggagga tttcatccat
     8461 gttgtatcta tgcatgcttg tcgcgcttca acagcccgct ccgtcgacgc cgtccggcgt
     8521 gacggtcgtt ccaccggctc cgcaggcgga cacgcagccg gctgagccgg ccgtcaccta
     8581 tgaccagggg ccactgcggg ctgaaatcct ggccatgttc gacgcgcgtt acttcttcct
     8641 ggaaaagacc gccggaacac gagaatcgga gttgcgcctg cagatgcgct tcgcgggcgc
     8701 cggaatcgag aacacggtac gcgtcgggaa tatcatcttc actgaagcga cggacgacac
     8761 gggtcgggcg ctggttacgc ctgaaacata cacggaagag caaaaaaccg agatgcgacc
     8821 gttcaatctg ccggcagagc ggctcaaggg cagcggactg acgcttcagg cgaagctcgg
     8881 ctcgccggcc cgggcggcgc aaacgatgcg cctgcgcggc tcgctgcgca ttgtcacggc
     8941 gcccgaaaag cttgagatct ccatcgagaa cccgttgcag tatgccggca agaccatccc
     9001 gaatgagcag ttgcaggcgg ccggtgtgga agtgcgcgtc gtcgcggccg aagaactgcc
     9061 ggaagagcaa tcccagccga atcaactggt gttgcagatc gtaaagggca gtgagaaggt
     9121 ccaggaagtc gcactttacg acgcttacat gaagcagatg cggccgcggg aacgcacgct
     9181 cgaaaccaag agcggcgact cggtcaaagg ctacagtatt accagcggta cgttggacga
     9241 gaacacgcaa ttggtgctgc gcgttttccc caggacggac gagatcacga ttccgatcga
     9301 gctggattcc gtgccgctgc cctgagcggc ggtttcgttg tctacgccca tgaggcggga
     9361 acagcgggcc gcgttttggc agaaatcagc accggcgcac ccgatcgaga aacgtccgtt
     9421 tatcgacgga cggttggcac cggagctgaa atttggctgg cctatcacga gggaccgcag
     9481 ttcccgcccg cgtgttcgtt cggacgcgct cactcaaaca gaatgcgctg tatgccgtta
     9541 caggcatgtc ggcgttctac gcctgccagt tagccgtcat cgtcatcctc gcgaaattcg
     9601 ccccgtcttt cgtactgggg caggtcgaat tcagcctggc ggtcgctacc ccggtcgtgc
     9661 tgttctgctc gctcgagctg cgcggcgcgt tcgtagctga tgccgcccac gaattcgctt
     9721 acggcaccta ccgaatgctg cgcaacctga tgatgggggt ggcggcggtt gtgttgctcg
     9781 ggtttgcggt ctgggaagcg gccacccagc aaaactggtc atacaccata atcctgatag
     9841 gcatgtgcgg cagccgcatc atgctttcgc aggccgaaat ggcatggggc ctgtttcaga
     9901 ggcgcgagcg tctggacctg atggcggcct cggcggcgca gcgcggggcg gccatgctgg
     9961 tcagttacgc cttgttcgtg ccgttgttcg cgtatctggc gcggcgcggt ctgattagcg
    10021 acggccacgg cgcggacggc gccgcgctgg cgatcgtggt ctacgtggcg gcgtcggcgg
    10081 ccattctggt gtggttcgat caccggcagg tagcggcccg ccacgacttt gatccgagtt
    10141 ggacctggac cgcggtgtgg aagctggcgc ggcggacgct gccgctgggg atagtcctgc
    10201 tccttttgca cctttcaagc agcgtcccgg tgtacgtcat cgagcaccag ccggggggca
    10261 tagcggccct gggacactac ggcgctctgg aaaagctcac c
//
LOCUS       DAOSYS010000123        10288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5526_length_10288_cov_5.197401, whole genome shotgun sequence.
ACCESSION   DAOSYS010000123 DAOSYS010000000
VERSION     DAOSYS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10288
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5526_length_10288_cov_5.197401"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1624)
                     /locus_tag="PLU99_08920"
     CDS             complement(128..1624)
                     /locus_tag="PLU99_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOO17218.1"
                     /translation="MTAKHSSRTGTGDLLAVGFGTTVLMWAIAYTSRLLELHLQTPAL
                     VFAILCLAAVLLLACHVGAGVLVGRYTSRGIAGGVKAGLLTGAINLLAVASAFGGDQP
                     NHLRPSAPLWIGGTLAAAVVLTGLGAAWGSFRRRPLPEPPDWHGGLAAVAACATFMLL
                     MIGGLVTGLDEGLAVVDWPNTETYNMFLYPLSRMTGGVFLEHAHRLFGSLVGLTTLVL
                     TIHVFRTEPRRAVRALALVALVCVAIQGTLGGLRVTGRFTFSADPADTQPSVVLAIVH
                     GVFGQMVFGLLVALALVRSRRWQRSTPSYVAAGAGVDRLLGVALLIALLAQLGFGALV
                     RHLTWALAKLRYELAAEPADMVSAGHWALTIHVTLAVLVALIAVAAGVRAWGLYERAA
                     ALRRSGLWLLAAVGCQLTLGVLALVVAGNDAPDNPPGLIDALITTAHQALGAALLALA
                     VLLLLWTWRLLATQPTAEAAGTCAIAPATAPTCAPLLERAATSSAGPR"
     gene            complement(1635..2030)
                     /locus_tag="PLU99_08925"
     CDS             complement(1635..2030)
                     /locus_tag="PLU99_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOO17219.1"
                     /translation="MTAPASPAIETAPARVGHFVPLWVLVLTLVALLALTAVTVGVTM
                     VPWLDLGHVGNLWIALLIATIKAILVGLFFMHLRYEKPFIAFILFVTLCFVGLFIGLV
                     LLDSTAYQPSIQQYREVDPVRYAPDLLNR"
     gene            complement(2042..2908)
                     /locus_tag="PLU99_08930"
     CDS             complement(2042..2908)
                     /locus_tag="PLU99_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOO17220.1"
                     /translation="MSTAAKTHASLTDTPQAHVAHHFHTASQQFEAAVQGMWIFLATE
                     ILLFSGLFCAYAVFRTNHPEVFEYAHQFLSKPLGAINTVILLCSSFTMAAAVWASQHG
                     RRRTAVALLCLTLLGGFGFLGIKFVEYEHKWKEGLLWGIHYQPHAENASAAAEAPAVA
                     FGEPAVPAAVSASENIDAAPAETGESDPAFRPASLGPPGLRRTAASAAPRTEQQVHNV
                     QTFFGVYFLMTGLHGLHVIAGMALIAWVAWRALKGAFSPAYFTPVAMVGLYWHLVDVI
                     WIYLFPLLYLIH"
     gene            complement(2915..4618)
                     /gene="ctaD"
                     /locus_tag="PLU99_08935"
     CDS             complement(2915..4618)
                     /gene="ctaD"
                     /locus_tag="PLU99_08935"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOO17221.1"
                     /translation="MSVIDPTVPTALPSSAEDAASDNYLTRPSGLRSWLFTVDHKRIG
                     VMYLVSILTAFLLGGIFAILLRTELLGPQKTVIEADTYNQFFTLHGAIMIFLVIIPSI
                     PATLGNFVLPIMLGAKDLAFPRLNLLSYYLYLGGALLALLAIVLGGVDTGWTFYPPYS
                     IEAAPYNGVIVVLAGVFLLGFSSILTGLNFVVTIHKLRPPGMGWFRMPLFLWSLYATS
                     IIQILATPVLGVTVLLLIVERTLKFGIFHPGLGGDPILYQHFFWFYSHPAVYIMILPA
                     MGIVSELVSVFSRKHIFGYPFIAFSSVAIAMFGFFVWGHHMFVSGQSPLVTVLFSLLT
                     YSVSIPSAVKVFNWLATMHGGSISLKTPMLYALGFVFLFGIGGLTGLFLGALATDVHL
                     HDTYFVVSHFHYVMLGSALIAMLGGMHYWWPKITGRMYSERWGVTAFVLVIIGFNLTF
                     FPQFIMGTHGMPRRYYNYPPEFTFLNQLSTTGSYIMAVSFVLMAAYLLHSLVRGRRAP
                     ANPWGGATLEWQCSSPPPTYNFETPPPVRDPYDFRNIEYDPASGGYRRIVPVPATVSK
                     G"
     gene            complement(4638..5624)
                     /gene="coxB"
                     /locus_tag="PLU99_08940"
     CDS             complement(4638..5624)
                     /gene="coxB"
                     /locus_tag="PLU99_08940"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOO17222.1"
                     /translation="MITGVLAQETAWPSVSPEGGTFWLPPEASSAASYTDDVFYFIYW
                     VSLFFFVLINALMVYFVIRYRRRTADQRAVSGATHNTRLEIVWSAVPLVLVVAMFYMG
                     FRGYMDLVNPPSEALEIHVLAQKWSWNFTYPNGHDDSELHVPVDHNIRLTLTSNDVIH
                     SLSIPAFRVRCDAVPGRYTHLWFRPTRIGEYLALCSEYCGTTHSDMIARVYVQDPAEY
                     AKWLADVSDPFREHSFADVGRMLVARRCSSCHSIDGRANVGPTFKSLFGREVKLADGT
                     TVTADENYIRESLIYPGRQIVAGYSPVMPTFRGQLKDREITAIIEYFKELAE"
     gene            complement(5621..6574)
                     /locus_tag="PLU99_08945"
     CDS             complement(5621..6574)
                     /locus_tag="PLU99_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOO17223.1"
                     /translation="MSGMSHGWFRAQWIFSLGVALLLTSCGVASAQDAIPESQPGVTS
                     SSSGGPPEATAGADAAGTERVEPLPEALEGVGITEHLNAKLPLDAKFMTHDRREVRLG
                     DFFDGKRPVILVLNYYRCPMLCGLVLNGLLESLKELEWTAGEQFEIVTVSFNPFEGPE
                     LANLKRANYLADYGRPAAANGWHFLVGRKDPIQRLLDTTGFGVKRDEASDQYVHAAAI
                     MLCTPDGRISRYLYGIVFEPRTLRLSLVEASEGKIGTTMDQLLLFCYHFEGGEYTLAA
                     MNVARAGALLTLLILGSVLVWLWRREVDRRRAAPPRQEPGA"
     gene            complement(6571..6984)
                     /locus_tag="PLU99_08950"
     CDS             complement(6571..6984)
                     /locus_tag="PLU99_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17224.1"
                     /translation="MADIEAGPGERDGDPNAHMVFTVGLIGAILMLALLIFGVVLFQN
                     VQALETEEKVFAGRPVELTQQQTLQLAAISEYRYISEADGVVAIPIDRAIDLFVARVR
                     EDPRQASPVAPASPDGQAQSESMEATPDTQPAGVP"
     gene            complement(7127..8362)
                     /locus_tag="PLU99_08955"
     CDS             complement(7127..8362)
                     /locus_tag="PLU99_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HOO17225.1"
                     /translation="MNARSINIEVESRPLTDAAPRLARIAGVLGLVGVVGAALFGFLA
                     GWDVFFRSYLLNYAYVLSLALGGLFFVLLQHVVRAGWSVSIRRVAELVAAALPPLTLL
                     FLPLLIPVVAGMPGVYEWTDQELVQADPLLKSKSAYLNVPFFIIRCAAYFGIWSLLAW
                     FCLWQSTEQDETGAAKHTQRLERLSAPGLLLLAVTITFFAIDALMSLNPHWYSTIWGV
                     YYFSGSVVGFLALFVLLLAGLQRAGRLTHAVTSEHYHDLGKLAFGFVVFWAYIAFSQY
                     LLIWYANIPEEIVWYRPRQGDTWWIAISLLLLFGHFVAPLLALMSRGAKRRSGFLVAA
                     AVWLLAMHWLDLYYVVAPRPWQSGHASSGGPLHATDLLLLLGLGGLFVAIVVWPMGRR
                     ALLAKRDPRLPEALAHENV"
     gene            complement(8367..9632)
                     /locus_tag="PLU99_08960"
     CDS             complement(8367..9632)
                     /locus_tag="PLU99_08960"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOO17226.1"
                     /translation="MTATKPQTAEPAVALQGLLAEFNSPGALLAAATQVRDAGFTCWD
                     THTPFPVHGIDHAMDIRRTRLPWLVFGLGVAGCLAGLLMQWWMNAASPAKFPGVPTFL
                     QGFDYLISGKPIFSLPANIPVIFELTVLAAALATVIGMLAANNLPWHYNALFAVPRFR
                     RVTNDRFFLCIDARDPQFDAAETGALLKQLGATAIERVETSARPGGLPRGVIIAIVVF
                     ATLALFPPLIVARARLSKSAQPRIALVQDMDNQERFRTQQANLAFADGRAMRPQIPGT
                     LARGDLHSDPHLYQGKTDGEWALTFPPQLEITRAFVQRGQQRFAIFCAPCHGLGGAGD
                     GMVHQRALQRDTPGWVQPTSLHDQLVRSRAAGHLFNTITNGIRSMPPYGDQIPPADRW
                     AIVAYVRALELSQDARLEDVPPERRDELR"
     gene            complement(9629..>10288)
                     /locus_tag="PLU99_08965"
     CDS             complement(9629..>10288)
                     /locus_tag="PLU99_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HOO17227.1"
                     /translation="GAIFGGFAMVMTLAIPARELFGLKHIITKRHLENCGKIVLVTCL
                     MVGYAYMMEFFMSWYSGSPYERFVFRSRAAGPYAWAYWTMIICNVAVPQLLWFKRIRT
                     NVWGMLAVVMAVNVGMWFERFVIIVTSLSQDYLPAAWDYYRPSLVEILTLAGSFGLFL
                     TLFLLFCRYLPMVAMAEVKTVMPQATEHHVAYVPVTRAFDYNPDEHEPPAELERGAAR
                     L"
BASE COUNT         2146 a         3228 c         3252 g         1662 t
ORIGIN      
        1 ccgcctgtcg gcaggggcat gcccgcgcgc gctgcgcgag gcttgggcat ggcacccagc
       61 aggggcatgg cacccggatt tcgcacccgg cctggggtgc cctttatcac tctatcacct
      121 tatcacccta tcgcggcccg gcactgcttg tagctgccct ttcaagcaat ggtgcacacg
      181 tcggcgctgt cgccggcgct atcgcgcatg tgccggcggc ttccgcggtc ggctgcgtgg
      241 ccagtaggcg ccaggtccag agcagcagca ataccgccag ggccaatagc gctgcgccga
      301 gggcttggtg ggcggtagtt atgagggcgt caatcagtcc gggcggattg tcgggggcgt
      361 cgtttccggc aactaccagg gccaggacgc ccagtgtgag ttggcaaccc accgccgcca
      421 gcagccagag gccgctacga cgcaaggccg cggcccgctc gtacagcccc caggcgcgca
      481 cgccggctgc gaccgcaatc agcgctacca gcaccgccaa agtgacgtgg atcgtaagag
      541 cccagtgccc ggcgctgacc atgtcggccg gttcggcggc caattcgtag cgcagcttgg
      601 caagggccca cgtcagatgt cgcaccagcg ctccgaaccc gagttgcgcg agcagcgcga
      661 tcagcagtgc tacgccaagc agccggtcca ctcccgcacc ggcggccacg taactgggag
      721 tgctgcgctg ccagcgtcgc gagcgcacta gcgccagggc cacaagcaag ccgaatacca
      781 tctgcccaaa gacgccgtgc acgatcgcga ggaccacgct gggctgcgtg tcggccggat
      841 cggcactgaa ggtgaagcgt ccggtgaccc gcaggccgcc gagtgtgccc tggattgcga
      901 cgcatactag cgcaaccagc gccagcgctc gcacggcgcg gcggggctcg gtgcggaaga
      961 cgtgaatcgt gagaacgagc gtcgtcagcc caaccagcga gccgaacagg cgatgggcgt
     1021 gttccaggaa gacgccgccg gtcatgcgcg acagcgggta caaaaacatg ttgtacgttt
     1081 cggtgttcgg ccaatccacg accgccagcc cctcgtccag accggtcacc agtccgccga
     1141 tcatcaggag catgaacgtc gcgcaggcgg ccaccgcggc caggccgccg tgccagtcgg
     1201 gcggttcggg cagcgggcgc cggcggaaac tgccccaggc cgctcccagc ccggtcagga
     1261 cgacggccgc cgccagcgtg ccgccgatcc acaacggcgc cgacggccgc aagtggttcg
     1321 gctggtcgcc gccgaaggcg ctggccaccg cgagcaggtt gattgcgccg gtcagcagtc
     1381 cggccttgac tccgccggcg atgccgcgtg acgtgtagcg gccgaccagg acgccagcgc
     1441 cgacgtggca tgccagcagc agtacggcgg ccaggcagag gattgcgaac actagggccg
     1501 gcgtttgcag gtgcagctcc agcaggcgcg aggtgtacgc aattgcccac atcagcacgg
     1561 tcgtgccgaa gccgacggcg agcagatcac cagtgccggt tcgcgatgaa tgcttggcgg
     1621 tcattctgca cgtcctagcg gttgagcaga tcgggcgcgt agcgcaccgg gtcgacctcg
     1681 cggtattgct ggatactggg ctggtaggcg gtggagtcca acagcaccag tccgatgaac
     1741 aagccgacaa agcagagcgt tacgaacagg atgaatgcaa tgaacggctt ctcgtagcgc
     1801 aagtgcatga agaaaaggcc gaccaatatc gccttgatgg tcgcgatcag cagggcgatc
     1861 cacaggttgc ccacgtggcc gagatccagc cagggcacca tggtcacgcc caccgtgacg
     1921 gcggtgagcg ccagcagcgc gaccagcgtc agaaccagca cccaaagcgg aacgaagtgg
     1981 ccgactctgg cgggggccgt ttcaattgcc gggcttgcgg gcgcggtcat gcgttcctcg
     2041 tttagtggat caggtatagt aatggaaaca gataaatcca gattacgtcc accaaatgcc
     2101 aatataggcc aaccattgcc acgggggtga aatacgccgg gctgaacgcg cccttcaagg
     2161 cgcgccacgc cacccatgcg atcaacgcca ttcccgcgat tacgtgcagc ccatgcagcc
     2221 cggtcatgag gaaatagacg ccaaagaacg tttggacatt gtggacctgt tgctcggtcc
     2281 ggggcgcagc ggaagcggcc gtccggcgca agccgggcgg gcccagcgat gccggtcgga
     2341 aggccgggtc ggactcgccc gtctccgccg gcgcggcgtc aatattttcc gaggcggaaa
     2401 ctgcggctgg gacggccggc tcaccgaacg cgaccgccgg cgcttcggcc gcggcagatg
     2461 cgttttcagc gtgcggctgg taatgaattc cccagagcag cccttctttc cacttgtgct
     2521 cgtactcgac gaacttaatc cccaagaagc cgaagccgcc gagcaacgtc aggcagagca
     2581 gcgcgaccgc ggttctgcgg cggccgtgtt gcgaggccca taccgcggcg gccatagtga
     2641 agctgctgca gagcaggatg actgtattga tcgcccccaa cggcttgctc aggaactggt
     2701 gcgcgtactc gaagacctca ggatggttgg tacgaaaaac ggcgtacgcg cagaacaagc
     2761 cgctgaacag cagaatttca gtcgccagga agatccacat gccctgaacc gcggcttcga
     2821 actgctgaga cgccgtgtga aagtggtgcg ccacatgcgc ctgaggcgtg tcagtcagcg
     2881 aggcgtgcgt cttggctgcg gtggacatgg cccgttatcc ctttgaaacc gtggccggca
     2941 ccggcacgat acggcgatac ccaccgctgg ccggatcgta ctcaatgttg cggaaatcat
     3001 aggggtcgcg caccggcggc ggagtctcaa aattgtacgt gggcggcggc gaactgcact
     3061 gccattcgag cgtcgccccg ccccaggggt tggccggggc gcgccgcccg cgcaccaggg
     3121 agtgcagcag ataggccgcc atcaggacaa agctgaccgc cattatgtat gaaccggtgg
     3181 tggacaactg attaagaaac gtaaactcgg gcggatagtt gtagtagcgg cgcggcatgc
     3241 cgtgggtgcc cattatgaac tgcgggaaaa aggtcaaatt gaagccgatt atgaccagca
     3301 caaacgccgt taccccccag cgctcgctgt acatccggcc ggttatcttg ggccaccagt
     3361 aatgcatgcc gcccagcatg gcgatcaggg cgctgcccag catgacgtag tgaaaatgcg
     3421 agaccacaaa atacgtgtcg tgcaggtgga cgtcggtcgc cagggctccc aagaacaggc
     3481 ccgtcaggcc gccgatgccg aacagaaaga cgaaccccag tgcatagagc atgggcgtct
     3541 tgagcgaaat cgaaccgccg tgcatggtcg ccagccagtt gaagaccttg acggccgacg
     3601 gaatggagac gctataggtc agcaggctaa acagcaccgt caccaggggc gactgaccgc
     3661 tgacgaacat gtgatgtccc cagacgaaga agccgaacat cgcaatggcg acgctgctga
     3721 acgcaatgaa tgggtagccg aatatgtgct tgcggctgaa aacgctgacc aactcgctga
     3781 cgatgcccat ggccggcaga atcatgatgt ataccgccgg gtgcgagtag aaccagaaga
     3841 aatgctgata caatatcggg tcgccgccca atccaggatg aaaaatgccg aatttcagcg
     3901 tgcgttccac gatcagcagc agcaccgtca cgcccagcac cggcgtggcc agaatctgaa
     3961 taatggacgt cgcgtacaac gaccacaaaa acaacggcat gcggaaccag cccattcccg
     4021 gcgggcgcag cttgtgaatg gtaacgacga aattcagccc ggtaagaatc gagctgaagc
     4081 ccagcagaaa aacgcccgcc agtacgacga tgactccgtt atacggcgcc gcttcaatcg
     4141 agtagggggg atagaacgtc caacccgtgt cgacgccgcc cagcacaatc gccagcagcg
     4201 ccagcagcgc gccgcccaag tacaggtaat agctgagcag gttcagccgc gggaaggcga
     4261 gatccttggc ccccaacata atcggcagca cgaaattccc gagcgtggcc ggaatgctgg
     4321 ggatgatgac caggaaaatc atgatcgcgc cgtgcagcgt gaagaactgg ttgtacgtat
     4381 ccgcctcgat gacggtcttc tgcgggccca gaagttcggt gcgcagcaga atggcgaaga
     4441 ttccgcccag cagaaacgcg gtcagaatcg aaaccaggta catcacgccg atgcgcttgt
     4501 ggtcgacggt gaaaagccac gaacgcagcc cgctcggacg ggtcaggtaa ttgtcagatg
     4561 ccgcgtcctc ggccgaggac ggcagggcag tcgggacagt cgggtcgatc acactcatca
     4621 acgccacctt gtcgcgctca ttcggccagc tccttgaagt actcaattat ggccgtgatc
     4681 tcgcggtcct tgagctggcc gcggaaagtc ggcatgaccg ggctgtagcc ggccacaatc
     4741 tggcgaccgg gatatatcaa cgattcgcga atgtagtttt cgtcggcagt gacggtcgtg
     4801 ccgtccgcca gcttgacctc gcgcccaaac aggctcttga aggtcggccc gacgttcgcc
     4861 cgcccgtcga tcgaatggca actgctgcac ctgcgggcca ccagcatgcg gccgacgtcg
     4921 gcgaacgagt gctcgcggaa ggggtctgag acgtccgcca gccacttggc atactcggcc
     4981 gggtcctgca cgtaaacgcg cgcgatcatg tcggagtggg ttgtgccgca atattccgag
     5041 cacagcgcca ggtactcgcc gattctggtc gggcggaacc acaagtgagt gtagcgtccg
     5101 ggaacggcgt cgcatctaac gcgaaacgcc ggtatagata agctgtgtat tacatcgttg
     5161 gacgtcagcg tcaggcgtat gttgtggtca accgggacgt gcagctcgct gtcatcatgg
     5221 ccgtttgggt aggtaaagtt ccagctccac ttctgcgcca gcacgtgtat ttcgagcgct
     5281 tcggacggcg ggttcacgag gtccatgtag cctcgaaagc ccatgtagaa cattgcgact
     5341 accagaacca ggggaactgc ggaccagacg atctccaggc gggtattgtg tgtggccccg
     5401 ctgaccgcgc gctggtctgc cgtccgcctg cgatagcgga taacgaagta gaccatcaac
     5461 gcattgatca ggacaaagaa aaacagcgag acccaataga tgaaatagaa gacgtcgtcc
     5521 gtgtagctgg cggcgctgga tgcttccggc ggcagccaga aagttccgcc ctcgggcgag
     5581 acgctgggcc atgcggtttc ttgtgccagc acgccggtaa tcatgccccg ggctcctgcc
     5641 tcggcggcgc ggctcgccgc cggtcaactt ctcgccgcca cagccacacc agcacactgc
     5701 ccaggatcaa cagggtcaac aaggccccgg cgcgcgctac gttcatcgcg gccagtgtgt
     5761 actcgccgcc ttcaaagtgg tagcagaaca gcagcaactg gtccatggtc gtgccgatct
     5821 tgccttccga cgcctccacc agcgaaagcc gcaacgtccg cggttcaaat actatgccgt
     5881 aaaggtaacg cgagatgcgc ccgtccggcg tgcagagcat tatggccgct gcgtgaacgt
     5941 actggtcgct ggcttcgtcg cgtttgacgc caaaccccgt ggtatccagc aagcgctgaa
     6001 tggggtcctt gcgcccgacg aggaaatgcc agccgttggc ggctgcgggc cggccgtaat
     6061 ccgccaggta gttggcgcgc ttaaggttgg cgagttcggg accttcgaac gggttgaagc
     6121 tgacggtaac gatttcgaac tgctccccgg cggtccactc cagttccttg agtgattcca
     6181 gcaggccgtt gagcaccagg ccgcacaaca tggggcagcg atagtaattc aagacgagaa
     6241 taaccggccg ctttccatcg aagaagtcgc ccagcctgac ctcgcgacgg tcgtgggtca
     6301 tgaatttcgc gtccagcggc agcttggcat tcaggtgctc ggtaatgccg acgccctcca
     6361 acgcctccgg caacggctcg acgcgctccg tccccgcagc atccgctccg gccgtagctt
     6421 ccggcggccc gccggacgac gacgatgtaa cgccgggctg cgattcggga atcgcgtcct
     6481 gtgcggaagc cacgccgcac gacgttagca gcagcgctac gccgagagag aagatccact
     6541 gcgcccggaa ccacccatga ctcattccgc tcatggtaca cccgccggtt gagtgtccgg
     6601 agtcgcctcc atagactcgc tctgcgcctg cccgtcgggg gaggcgggcg cgacgggaga
     6661 ggcctgtcgc gggtcttctc gtacgcgcgc cacaaacaga tcaatggccc gatcgattgg
     6721 aatagcgacc actccgtcgg cctcgctgat gtaacggtac tcgctaatcg cggcaagctg
     6781 caacgtctgc tgctgcgtca actcgacggg ccggccggca aaaaccttct cttcggtctc
     6841 gagggcctgc acattctgaa agagcacgac cccgaaaatc agcagcgcca gcatcagaat
     6901 cgccccgatc aggccgacgg tgaacaccat gtgggcattc ggatcgccgt cgcgctcgcc
     6961 cggtccggct tcgatatcag ccatttcggc tgtgtatcgg gacccggcga cctctccgtc
     7021 cgttagtggc gcggacgtcc cgccgatcga gcgcgtaacg tccggaggct cgcgggacgt
     7081 gctcccgggc tcggcggatg aattcattgg gccgggtgtt tccatgtcaa acgttctcgt
     7141 gcgccaaagc ttcgggcaaa cgcgggtcac gcttggccaa cagtgctcgg cggcccatcg
     7201 gccacactac gattgccaca aacaggccgc ccaggcccag caacaacagc agatcggtcg
     7261 cgtgcagagg cccgccggac gaggcgtgcc cgctctgcca cggccgcggc gcgacgacgt
     7321 agtacagatc cagccagtgc atggccagga gccagactgc ggccgcaacc agaaagccgg
     7381 accggcgttt ggcgccgcgg gacatcagcg cgagcagggg ggccacgaag tgcccgaaga
     7441 gcaatagcag gcttatggcg atccaccagg tgtcgccttg gcgcgggcga taccagacaa
     7501 tttcctcggg gatattggca taccagatca gcaggtattg cgaaaaggcg atgtacgccc
     7561 agaacaccac gaatccaaag gccagcttgc cgaggtcgtg atagtgctct gaggtcaccg
     7621 cgtgcgtgag gcggccggcc cgctggagac ccgccaatag caggacgaac agcgccaaaa
     7681 agcccaccac gctgccggag aagtagtaga cgccccaaat ggtgctgtac caatgcggat
     7741 tcagcgacat cagggcgtca atcgcgaaaa acgtgatcgt caccgccagc agcagcaggc
     7801 cgggcgcgct gagccgttcc agacgctgcg tgtgttttgc cgctccggtt tcgtcctgtt
     7861 cagtagattg ccacaggcag aaccacgcca gcagagacca aatgccaaaa taggcggcac
     7921 agcgaataat gaagaacggc acattcagat aggccgattt cgattttaga agcgggtcgg
     7981 cctgcaccaa ctcctggtcc gtccattcgt atacgccggg cataccggcg accactggta
     8041 tgagcagtgg cagaaagagc agtgtcagcg gtggcagcgc tgccgccacg agttccgcga
     8101 cgcggcggat cgaaacgctc caaccggcgc gcaccacgtg ctgtagcaag acaaagaaga
     8161 gtccacccaa cgccagactc agcacatagg cgtagttgag caaatacgag cggaagaaga
     8221 cgtcccaacc cgccaggaag ccgaacaggg ccgcgccgac aacgcccaca agccccagca
     8281 cgcccgcgat tctggccagg cgcggcgccg cgtccgtcag cggccgagat tcaacttcaa
     8341 tgttaattga cctggcgttc acgttcctac cgcaactcgt cgcgtcgctc gggcggcaca
     8401 tcctccagcc gagcgtcctg gctgagctct aacgcccgca cgtatgccac aatcgcccac
     8461 cggtccgccg gcggaatctg gtcgccatag ggcggcatac tgcggatgcc gttcgtaatc
     8521 gtattgaaca ggtgtccggc cgcgcggctg cggacaagct gatcgtgcag cgaagtcggc
     8581 tggacccaac ccggcgtgtc gcgctgcaag gcccgctggt ggaccatgcc gtcgccggcc
     8641 ccgcccagcc catggcacgg ggcgcagaat attgcgaagc gctgttgccc gcgctggacg
     8701 aatgctcttg tgatctcaag ctgaggcggg aaagtcagcg cccattcgcc gtcagtctta
     8761 ccctggtaca ggtgcgggtc cgaatgcaga tcgccgcgcg cgagcgtgcc cggaatttgc
     8821 ggccgcatgg cgcgcccgtc ggcgaacgca aggttggcct gctgggtcct aaagcgttcc
     8881 tggttgtcca tgtcctgcac aagcgcgatg cgcggctggg cggacttcga gaggcgtgcc
     8941 cgggccacga tcagcggcgg gaacagggcc agggtagcga agaccacgat ggcgattatc
     9001 acgccgcgtg gcagcccgcc gggcctggcg cttgtctcga cgcgttcgat ggccgtcgcg
     9061 ccgagttgtt tcagcagtgc gccggtttcc gcagcgtcga actgcgggtc gcgggcatct
     9121 atgcacagga aaaaacggtc attggtgacg cgccggaagc gcggcacggc aaatagcgca
     9181 ttgtagtgcc acggcagatt attggccgcc agcatcccga ttacagtggc cagagcggcc
     9241 gccagaaccg tcagttcaaa gatgaccgga atattcgccg gcaggctgaa gatcggcttg
     9301 ccgctgataa gatagtcgaa gccttgcagg aatgtcggca cgccaggaaa tttcgccggc
     9361 gacgccgcat tcatccacca ttgcatcagt aagcccgcca ggcagccggc cacgcccaga
     9421 ccgaagacca gccacggcaa gcgagtgcgg cgaatgtcca tggcgtggtc tatgccgtgg
     9481 acggggaacg gcgtgtgcgt gtcccagcat gtaaagccgg cgtcacgcac ctgcgttgcc
     9541 gccgccaaca acgcgccggg cgagttgaat tcggccagta gcccctgcag ggcgacggcg
     9601 ggttcggcgg tctgcggctt ggtcgcggtc atagccgtgc cgctcctcgt tccaactctg
     9661 ccggcggttc gtgttcatcc gggttatagt cgaaagcgcg cgtgaccgga acgtacgcga
     9721 cgtggtgctc ggtcgcttgc ggcatgacgg tcttgacttc agccatcgcg accatcggca
     9781 gatagcggca gaacaacaaa aatagcgtga ggaacaggcc gaaacttccc gcgagcgtca
     9841 agatttccac gagcgacggc cggtaataat cccaggccgc cggcaagtag tcctgcgaca
     9901 gcgacgtgac aatgatcacg aagcgctcaa accacattcc gacgttcacg gccattacca
     9961 ccgccagcat gccccaaacg ttcgtgcgaa tgcgcttgaa ccacaggagc tgcggcacgg
    10021 ccacgttgca gataatcatg gtccagtagg cccaggcata cgggccggcg gcgcgggagc
    10081 gaaagacgaa acgctcgtag ggcgagccgc tataccagga catgaagaat tccatcatgt
    10141 aggcgtagcc caccatcagg cacgtcacca gcacgatctt gccgcagttc tccaggtgcc
    10201 gcttggtgat aatgtgcttg aggccgaata gctcgcgcgc cggaatggcc aacgtcatca
    10261 ccattgcgaa gccgccgaag atggcgcc
//
LOCUS       DAOSYS010000124        10240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5565_length_10240_cov_4.569072, whole genome shotgun sequence.
ACCESSION   DAOSYS010000124 DAOSYS010000000
VERSION     DAOSYS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_10240_cov_4.569072"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLU99_08970"
     CDS             complement(<1..261)
                     /locus_tag="PLU99_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17228.1"
                     /translation="MSRRKPRSGAARWRRRIQLLSLAAFFALVLLARPTPGQNPSPLL
                     KLFFYLDPLILLSTALAAHTLQVAALLALITVAVTLAFGRVFC"
     gene            complement(258..>750)
                     /locus_tag="PLU99_08975"
     CDS             complement(258..>750)
                     /locus_tag="PLU99_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO17229.1"
                     /translation="IVYLDKTRFRDMKVGGNRLKQHPVYPGIVECDLVINVPIAKHHS
                     ATTVTACMKNYMGVVEKRREFHQDLPTTIADITQFMKPRLCVLDATRVLTAHGPTGGD
                     LADVKVLNTVAAGVDIVALDAFGCELLGYKPAEIETVTTGQKYGLGKIDYRSLALKEF
                     ELS"
     assembly_gap    751..760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<761..1243)
                     /locus_tag="PLU99_08980"
     CDS             complement(<761..1243)
                     /locus_tag="PLU99_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO17230.1"
                     /translation="MPEQSRREFLVRSAVLGVAALGTRKLAWGQPAPAGQQPAAGGGD
                     RPLDMTIVRFKGEMSTADAVGETSPAARLTEKALAELGGMQRFVKKGDVVWVKPNIGW
                     NRTAEQAANTHPEVVAALVRLCLNAGAKKVKVGDYPCNEAKETYENSGIAAAAQAAGA
                     E"
     gene            complement(1554..>4048)
                     /locus_tag="PLU99_08985"
     CDS             complement(1554..>4048)
                     /locus_tag="PLU99_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HOO17231.1"
                     /translation="GENFAGFMHLVDYEEALVGGRIWTETEHIEARLTELTSHMENVI
                     QKYLRNEFDSIDAYNRQAGDLAEPYRFLVIADFPANFNEESIRRLTSIVSSGARCGVF
                     TLIAHDRRLPAPAGLCIEDLTSAGLHLVYQGDRFVLQDDLLRHFPLTLDAPPSEEVLT
                     QIMHAVGSAAKDALRVELPFDSIVPPPAQLWSADARNEVAVPLGNAGAVRLQHLRLGR
                     GLAQHALIAGKTGSGKSNLLHVIITNLALWYSPDEVEFYLVDFKKGVEFKVYVTQPLP
                     HARAVAIESDREFGVSVLQRIDAELSRRGELFRAAGVQDLPAYRQVTKAVLPRTLLIV
                     DEFQVFFTEDDRLSHDAGVLLDRLVRQGRAFGIHVILGSQTLGGSTGLARSTVGQMAV
                     RIALQCSEADSQLILDDTNVAARLLSRPGEAIYNDAGGLVAGNSPFQVAFLPDEVRNT
                     HLERVIALARASAAKPAAGEPKGARFWMREPAIVFEGNAPADIRQNRLLARLLESPAA
                     ATAHGREAGAVATLAPQAWLGEAVAIKDPTAAVFRRQSGDNLLIVGQRDDAALALMAS
                     TMVSLAAQLPPQAARFVVLDVRPAIEERAESESTGTPSGRQDALGTSSSDAGSLTGAG
                     SVTGATRLSGGKGQPITELREVAAALPHGTRFVAWRDVAAAIGELTAETQRRVQADDL
                     GAPAVFLFIFGLQRYRMLRRREEEFSFSADREDQPPAPDKQFAELLREGPPVGVHVVA
                     WSDTLATLERTLERQSVGEFDYRVLFQMSAADSSHLIDTLDANRLGFYRALLYSEEHG
                     LFEKFRPYAAPDTAWLSQAATFLSHKWQAAEQ"
     assembly_gap    4049..4148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4149..5529)
                     /locus_tag="PLU99_08990"
     CDS             complement(<4149..5529)
                     /locus_tag="PLU99_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17232.1"
                     /translation="MPQTSNPSPPPKPITNGEAYTEIQRAAFRELLNLSADTVAPLEA
                     EIEQRLRQGCAAADARLAELTAKAEHRLKEELADAQRVHAERVQQAEAEYTAAEESLA
                     RQVQQTQENIAAGYQEAQRKAQRQREDNLLMIDTVARATLQAFADKRQALERDVSWGR
                     SQLEGLQQQGLRVLEEHEYDLQRDPIAPVEEPWAGDDPAAECKSHAASAARSLAAMRA
                     LMMPRLLVGPAVFAGLTLLNVVPLAVLPILGSFAVAPAQTLMIAAPIVLTLTLMISVI
                     AGYFIRRKARLLIRAQLQAEYARFSGALLVAGRALELHWERAKEQLRQDEAKAVARHE
                     RDKRAACEQYDAGMAKADKRRDEVLQSIQADRDIQHAKNEQRRAAALRQAEEELAQLT
                     RKYQEQHDQAVAAARRQHERATENSRHQHEAARASLTASWTDGLARIRRFLDDTDQLA
                     KLPGLHSSDG"
     gene            complement(5703..5915)
                     /locus_tag="PLU99_08995"
     CDS             complement(5703..5915)
                     /locus_tag="PLU99_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17233.1"
                     /translation="MSVTAGLGKLRTAAKDLRLQWNEVQVEWHDDNMRRFQTNHIEPL
                     FVRVRMVELALAQMASVLEKARQDCG"
     gene            complement(5919..6956)
                     /locus_tag="PLU99_09000"
     CDS             complement(5919..6956)
                     /locus_tag="PLU99_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17234.1"
                     /translation="MGDLVRVESVEALKRFRAAFCKFAEIATTGLDEAEFEVQRTSFW
                     VKQDRQAYWKRQVSHRTELLTRARLALKQKESQRTPLGGRYSCVDEMKAVAAAQRALD
                     EACQKQANVQRWSRVLDEERFSFQAQTQALRVALQADIPNALAQLDQMIAALEGYAST
                     AIPQQQTSTAPQIPEETSAATELPSMARTPPAPPAPGAAQYELLHARTPSQAIRDATP
                     VTAPGPHEVVANAASSDSAAVLSACGLPQTPCDADAKIVLARGVLQSRHTYLERIDGA
                     AQDDSGWFVGFADDSQAAAYDALRVADLLALRPDWAAVLALPPGCLVTCDGRELQAVY
                     GPHGNLLWSAR"
     gene            complement(7123..7395)
                     /locus_tag="PLU99_09005"
     CDS             complement(7123..7395)
                     /locus_tag="PLU99_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HOO17235.1"
                     /translation="MAKAHVDPTELRRFARDLTKFSQELRTLMGGLNASLRNLEKTWR
                     DQEQRKFTEEFEQTMKTLGRFLESTERHVSFLQQRAGHIEEYLQQR"
     gene            complement(7495..9432)
                     /locus_tag="PLU99_09010"
     CDS             complement(7495..9432)
                     /locus_tag="PLU99_09010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOO17236.1"
                     /translation="MRRVFGFEDFRAGQQAVIERLLARRNVLAIFPTGGGKSLCYELP
                     ALLFDGLTVIISPLIALMKDQVEFLTARSVPAARLDSSLGPDETRGVYEGLRSGTLRL
                     LYVSPERLGNERFLHLLEGRRISLLAIDEAHCISEWGHNFRPDYLKIARLARQLKVER
                     VLALTATATPTVAGDITREFGITKNDVVHTGFYRPNLNLRVTPCAAQERLELLLSRVE
                     QRPAGPTVVYVTLQRTAEEVAQALCKRGFAAQAYHAGLDAEVRNSVQDAFMASDRLIV
                     VATIAFGMGVDKANIRYVYHYNLPKSLESYMQEVGRAGRDGQPAICELLACADDVVTL
                     ENFSYGDTPTPGAVAALLDEILDQDERFDVSVADLSYRHDMRPLVVETLLTYLELEGV
                     LRATGPFYTEFKFAPRRPSQDILRDFDAERAAFLRAVFQQARQGRTWFTLDVEAASQA
                     LGQPRERIVKALGYLEEKGDLLLQLAGVRQGYRREPKSIDRQALSATLSARFVRREEH
                     DIARIRSVVRWAEQPGCLTARLLRYFGETRQDCGHCSRCEEEAHRGTGAKPAGAGAQA
                     VSLPATRRAPLGEREAARLRKLRAEKHAALATPRQLARFLCGLSSPATTRAKLRKHAD
                     FGLMESTPFSEVLAFVEHCAS"
     gene            complement(9868..>10240)
                     /locus_tag="PLU99_09015"
     CDS             complement(9868..>10240)
                     /locus_tag="PLU99_09015"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOO17237.1"
                     /translation="WRWPPLGGSDRGTLMKTITKKHLVDRISEQTNYKRIVVKRVIQQ
                     FLEEIVRELGQGNRLEFRDFGVFELRERAARVAQNPKTMEKVHVPSKRTVKFKVGRMM
                     RDRLLLDHRPRKADQPAGTAS"
BASE COUNT         1685 a         3266 c         3365 g         1814 t
ORIGIN      
        1 gcagaacacg cggccgaagg ctagcgtcac ggcgactgtg atcagcgcca gcagcgccgc
       61 gacttgcaag gtgtgcgccg caagggccgt ggacaacagg atcagcggat ctaggtagaa
      121 gaacagcttc agcagcggcg agggattttg cccgggtgtg gggcgtgcca acagcacgag
      181 cgcgaagaac gcggcgagcg acagcagttg aatccgccgg cgccaccgag cggccccgga
      241 gcgaggcttg cgacgactca cgagagctcg aattccttca aagccagact gcgatagtcg
      301 atcttgccca acccgtactt ctggccggtg gtgaccgttt cgatctcggc cggcttatag
      361 cccagaagtt cacagccgaa agcgtccaat gcgacgatat caacgccggc cgcgaccgtg
      421 ttcagcacct taacatcggc caggtcgccg ccggtggggc cgtgggccgt gaggacgcgg
      481 gtcgcgtcga gcacgcagag gcgcggcttc atgaattgtg tgatgtcggc gatggtcgtg
      541 ggcaggtcct ggtggaactc gcgccgtttc tcgaccacgc ccatgtagtt cttcatgcag
      601 gctgtaacag tggtagccga gtgatgcttg gcgatgggca cgttgataac caggtcgcac
      661 tcgacgatgc cggggtagac cgggtgctgc ttgaggcggt tgccgccgac tttcatgtcg
      721 cggaagcggg tcttgtcgag gtagacgatt nnnnnnnnnn ttcggcgccg gccgcctgcg
      781 ccgcggccgc gatgccgctg ttttcatacg tctccttggc ctcgttgcag gggtagtcgc
      841 cgaccttgac cttcttcgcg ccggcgttca ggcacaggcg caccagggcg gccacgacct
      901 ccgggtgcgt gttggcggcc tgttcggcgg tgcggttcca gccgatgttg ggcttgaccc
      961 agacgacgtc gccttttttg acgaagcgct gcattccgcc gagttcggcg agggcctttt
     1021 cggtcaggcg tgcggcgggg ctcgtctcgc ccaccgcgtc cgcggtcgac atctcgccct
     1081 tgaagcggac gatggtcata tccaggggcc ggtcgccacc cccagcggca ggctgctggc
     1141 cggccggagc gggttggccc caagccagct tgcgtgtccc aagcgcggcg acgcccaaca
     1201 cggctgaacg gacgagaaac tctctccgag actgctcggg catggttaac ctccggctcc
     1261 gaacttcggc tgtgagactt ttaccacggc aggcgggccg cccgcaacta aaatcgagac
     1321 gcagccaaaa tcggggtacg gtcggaatcg agctgcggca tgaaccgagg tgcaaccggg
     1381 gggcgtccag agcagagccg gggcgtgccg ccccggaatg ccgccaacaa ccgccacctc
     1441 atcgcaaacc gcgcgcgggt gtcagagtga taaagtggta aggtgataga gtgataaggg
     1501 gtcgcgcaga tcgcccttta tcactctatc actttatcac cttatcacgc gttttactgt
     1561 tcggcggcct gccacttgtg gctcaggaat gtggctgcct gcgatagcca ggcggtgtcg
     1621 ggggcggcgt aggggcggaa tttttcgaac aggccgtgct cttcgctgta gagcaaagcg
     1681 cggtagaagc ccaggcggtt tgcgtccagc gtgtcgatca ggtggctgga atcggcggcg
     1741 ctcatctgga acaggacgcg atagtcgaac tcgccgaccg actgccgctc cagcgtgcgc
     1801 tcgagcgtgg ccagcgtgtc cgaccaggcc acgacgtgca cccccaccgg cggcccctcg
     1861 cgcagtagct ccgcgaattg cttgtcgggc gccgggggct ggtcctcgcg gtcggcggag
     1921 aagctgaact cttcctcgcg ccgccgcagc atgcggtaac gttgcaggcc gaaaatgaac
     1981 aggaaaacgg ccggggcgcc cagatcgtcg gcttgcacgc ggcgctgcgt ctccgcggtc
     2041 aactccccaa tggcagccgc gacatcgcgc caggcgacga agcgcgtccc gtgcggcaac
     2101 gcggccgcaa cttcgcgcaa ctccgtgatg ggttggccct taccgcccga cagtctcgtg
     2161 gccccggtca cgctacctgc gccagtcagg ctgccggcgt cggatgagct tgtgcccaag
     2221 gcgtcttggc gtcctgaagg tgtgccggtt gactcgctct ccgcacgctc ctcaatcgcc
     2281 gggcgcacgt ccagaacgac gaaacgagcc gcttgcggcg gcagttgggc ggccagcgaa
     2341 accattgtgg aagccatgag cgcaagcgcg gcgtcgtcgc gctggccgac aatcaacagg
     2401 ttgtcgccgc tctgacggcg gaaaaccgcg gcggtcgggt ctttgatggc gaccgcttca
     2461 ccaagccacg cttgcggggc aagtgtggcc acggcgccgg cctctcgccc atgcgccgtg
     2521 gcggcagccg gcgattccag cagccgggcc agcagacggt tctggcgaat gtcggccggg
     2581 gcgttgccct cgaagacgat cgccggctca cgcatccaaa agcgggcgcc tttcggctcg
     2641 ccggccgccg gcttggctgc agaagcgcgg gccaaagcaa tgacacgttc caagtgcgtg
     2701 ttgcggactt catccggcaa gaacgcgact tgaaacgggc tgttgccggc caccaggcca
     2761 cccgcgtcgt tgtagatggc ttcgcccggc cgcgacagca gccgtgcggc gacgttggtg
     2821 tcgtccagga tgagctgtga gtcggcctcg gagcactgca gcgcgatgcg aaccgccatc
     2881 tggccgacgg tgctgcgggc caggccggtc gagccgccca acgtttgcga gccgagtatc
     2941 acgtggatgc cgaaggcgcg gccttggcgc accagccggt cgagcagcac gccggcatcg
     3001 tgtgagagtc ggtcatcctc cgtgaagaag acctggaact catcgacgat cagcagcgtg
     3061 cgcggcagca ccgcctttgt tacctggcgg taggccggca agtcctgcac gccggccgcg
     3121 cggaacagct cgccgcggcg cgataactcc gcatcgatgc gctgcagcac gctgacgccg
     3181 aactcgcgat cgctctcgat cgcgaccgcg cgggcatgcg gcaacggctg cgtgacgtat
     3241 accttgaatt cgacgccctt cttgaaatcg accaggtaga actcgacttc gtccgggctg
     3301 taccacaggg cgagattcgt aatgatgacg tgcaacaggt tcgacttgcc cgaaccggtc
     3361 ttgccggcga tcagggcgtg ctgggccaaa ccgcggccga gacgcaaatg ttgcaggcgg
     3421 acggcgccgg cgtttccgag cggaacggcg acctcgttgc gggcgtcggc ggaccacagt
     3481 tgcgcggggg gcggcacgat cgaatcaaac ggcaattcga cgcgcaaagc gtctttggcc
     3541 gcgctgccca ccgcgtgcat gatctgcgtc agcacttctt cagacggcgg cgcatcgagc
     3601 gtcagcggga agtgccgcag cagatcgtcc tgcaggacga accggtcgcc ttggtacacg
     3661 aggtgcaagc cggcgctggt cagatcctcg atgcacaagc cggccggcgc gggcaggcgg
     3721 cgatcgtggg cgatgagcgt gaagactccg caacgggcgc cgctgctgac gatgctggtc
     3781 aggcggcgga tcgactcttc gttgaagttg gccggaaagt cggcgatcac caggaagcga
     3841 tacggctcgg ccaggtcgcc ggcctggcgg ttgtaggcgt cgatgctgtc gaattcgttg
     3901 cggaggtact tctggatgac gttctccatg tggctggtca gctcggtgag gcgggcctcg
     3961 atgtgttcgg tctcggtcca gatgcgtccg ccgaccaaag cctcttcgta atcgaccagg
     4021 tgcatgaagc cggcgaagtt ttcgcccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnngg ccgtcggaag aatgcaggcc cggcagcttg gcgagctggt ctgtgtcgtc
     4201 gaggaaccgc cgaatgcggg cgaggccatc cgtccaggag gcggtgaggc tggcgcgggc
     4261 ggcttcgtgc tggtggcggc tgttttcggt ggcgcgctcg tgttggcgtc gcgccgccgc
     4321 cacggcctga tcgtgctgct cctggtactt gcgcgtgagc tgcgccaact cctcctcggc
     4381 ttggcgcagc gcggccgcgc ggcgctgctc attcttcgcg tgctggatgt cgcggtcggc
     4441 ttggatgctc tggagaacct cgtcgcggcg tttgtcggct ttggccatgc ccgcgtcgta
     4501 ctgctcgcaa gcggcgcgtt tatcgcgttc gtgccgcgcg acggctttgg cttcgtcctg
     4561 gcgcagttgc tccttggcac gctcccaatg cagttccagg gcacggccgg cgaccagcag
     4621 cgcgccgctg aagcgcgcat attctgcttg gagctgagcg cgaatcagca ggcgggcttt
     4681 tcggcggatg aagtaaccgg caatgacgct gatcatcagc gtgagggtca gaacgatcgg
     4741 cgcggcaatc atcagcgttt gggcaggcgc caccgcgaac gatccgagaa tcggcagaac
     4801 tgccagcggg accacgttga gcaaagtgag cccggcgaag accgcgggac cgaccaatag
     4861 ccggggcatc atcagcgctc gcatcgcagc cagcgatcgg gcggcgctgg cggcgtgcga
     4921 cttgcactcg gcggcgggat catcgccggc ccagggctct tccacgggcg cgatcgggtc
     4981 gcgctggagg tcgtattcgt gttcctccag gacccggagg ccctgttgtt gaaggccctc
     5041 caactggctc cgtccccagg acacatcgcg ctcgagcgct tggcgcttgt cggcgaacgc
     5101 ctgcagcgtg gcccgcgcga ccgtgtcgat catcagcaaa ttgtcttcgc gctgccgctg
     5161 ggccttccgc tgggcctcct ggtaccccgc cgcgatgttc tcctgcgttt gctgtacctg
     5221 ccgggccaga gactcttcgg ccgcagtgta ctcggcttcc gcctgctgca cgcgctcggc
     5281 atgcacgcgc tgcgcgtcgg ccagctcttc tttcagacgg tgctcggctt tggcggtgag
     5341 ttcggccagc cgcgcgtcgg cagcggcgca cccctggcgc aagcgctgct cgatttcggc
     5401 ttcgagcggc gccacggtgt cggccgagag gttcagtagc tcgcggaagg cggcgcgctg
     5461 gatttcggta taggcttcgc cgttggtgat tggcttgggc ggcgggcttg ggttgctggt
     5521 ttgtggcatg atcctcaaat cacccggcgt tattcgatac taatgcgaat gcggcgctgc
     5581 ttgaaacagc cgcactgctt gaagcttcgt atcaagcagt ggcacacaca cgcacataca
     5641 cagcccacac gcacagcacg cacatatacc agcacataca aacacagcag gcacgtagtc
     5701 ggtcatccgc aatcctggcg cgccttttcc aatacgctcg ccatctgcgc cagggctaat
     5761 tctaccatcc gcacccgcac gaacagcggt tcaatgtgat tagtctggaa acggcgcatg
     5821 ttgtcgtcgt gccactcaac ctgcacctcg ttccactgca gccgcaaatc cttggcggcg
     5881 gtcctgagtt tgcccaatcc ggccgtgacg ctcatggatt acctcgcaga ccacaacaag
     5941 ttgccgtgcg ggccatacac ggcctgaagc tcccggccgt cgcaggtaac caggcagccg
     6001 ggcggcagcg ccagcaccgc ggcccaatct ggacgcaggg cgagcaaatc ggccacgcgc
     6061 agcgcatcgt aggccgcggc ttgtgagtcg tccgcgaatc cgacgaacca gccgctgtcg
     6121 tcctgagccg cgccgtcaat tcgctcaagg tatgtatgcc gcgattgcag gacgccgcgg
     6181 gccaacacga ttttggcgtc ggcgtcgcac ggggtctggg gcaggccgca ggcggaaagt
     6241 acggcggcgc tgtcgctgct ggcggcattc gcgacaacct cgtgcgggcc gggcgcggtg
     6301 acgggcgtgg cgtcgcgtat agcttgtgat ggagttcgcg cgtggagaag ctcgtattgg
     6361 gcggcgccgg gcgcgggcgg ggccggcggc gtgcgggcca tcgagggcag ctcggtcgcc
     6421 gcgcttgtct cttccggtat ctgcggggcg gttgaggtct gctgctgtgg aatggcggtc
     6481 gaggcatatc cttccaacgc ggcgatcatc tggtcgagtt gggcgagggc gttaggaatg
     6541 tccgcctgga gcgcgacgcg cagagcctgc gtctgagctt ggaacgagaa gcgctcctcg
     6601 tccagaacgc ggctccagcg ttgcacgttg gcctgctttt ggcaggcttc gtcgagggca
     6661 cgctgcgcgg cggccacggc cttcatctcg tccacgcacg aataccgacc gccgagcggt
     6721 gttcgctgcg attccttctg cttgagggcg aggcgggcgc gcgtgagcaa ctcggtgcga
     6781 tgcgacacct ggcgtttcca gtaggcctgc cggtcctgct tgacccagaa gcttgtgcgc
     6841 tgcacctcga attcggcctc gtccaggccg gtggtggcga tttccgcgaa tttgcagaag
     6901 gcagctcgga atcttttgag agcctcgacg gattcgacgc gaaccagatc gcccatttta
     6961 agtttctacc gctgcgaggt tttacgtcgg ccacgggggg ccggcgctac ctatacgttg
     7021 gccacaaggg gccggcgccc gcgggagccc ggccctacct atacgcacgt cggccacggg
     7081 ggtcggcgtt accagtacgt cggccgcggg ggtcggcgct actcatcgct gctgcaggta
     7141 ctcctcaatg tgcccggcgc gttgctggag aaaggagaca tgccgctcgg tggactccag
     7201 aaagcggccc agcgttttca tggtctgctc gaattcttcg gtgaacttac gctgctcctg
     7261 gtcgcgccag gtcttctcca ggttgcgcag gctggcgttc aggccgccca tcagcgtccg
     7321 aagctcctgg ctgaatttgg tcagatcgcg ggcgaaacgc cgcagttcgg tcgggtcaac
     7381 atgggctttg gccatacgcg cctcccgcgc ggtaacaagc ggctgccgtc tcgccgcgca
     7441 ttgtaacgcg cgaagcgcgg cgcggcacca ttatccgaca gttgagccgc tcagctaact
     7501 ggcgcagtgc tcgacgaacg ccagcacttc cgaaaacggc gtcgactcca tcaggccgaa
     7561 atcggcgtgc ttgcgcagct tggcgcgcgt ggtggccggc gaggacaggc cgcacaggaa
     7621 gcgggccagt tgccgcggcg tggccagggc agcgtgcttc tcagcgcgga gcttgcgcag
     7681 ccgcgcggct tccctttcgc ccaacggcgc gcggcgcgtg gcgggcaggc tgaccgcttg
     7741 agcaccggca ccggccggtt tcgcaccggt cccgcggtgt gcttcttcct cacagcgcga
     7801 gcaatggccg caatcctggc gggtttcgcc gaaataccgc agcaggcggg ccgtcaaaca
     7861 accaggctgc tcggcccacc tcacgacact acggatgcgc gcgatgtcgt gctcctcgcg
     7921 gcggacgaag cgggcgctca gcgtcgcgct tagcgcctgg cggtcgatcg acttcggttc
     7981 gcgacggtag ccctgccgca ctccggccag ttgcaaaagc aggtcgccct tctcttccag
     8041 gtaccccagc gccttgacga tgcgctcgcg cggctggccc agcgcttggc tggcggcctc
     8101 gacgtccagt gtgaaccatg tgcggccctg gcgcgcctgc tggaaaacgg cccgcagaaa
     8161 cgcggcccgc tcggcatcga aatcgcgcag gatgtcctgc gacggccgcc gcggcgcgaa
     8221 cttgaattcg gtataaaacg gcccggtcgc gcgcagcacg ccctccagct ccaggtacgt
     8281 caacagcgtt tccacgacca gcggacgcat gtcgtgccga tacgacaggt cggccaccga
     8341 cacgtcgaac cgttcgtcct gatcgagtat ctcatcgagc agcgccgcca ccgcgcccgg
     8401 ggtcggcgta tcgccgtatg aaaagttctc gagtgtgacg acgtcgtccg cgcaggctaa
     8461 caactcgcat atcgcgggct gaccatcccg cccggcccgt cccacctcct gcatgtagct
     8521 ttccaaactc ttaggcaggt tgtagtgata gacgtagcgt atgttcgcct tgtccacgcc
     8581 catgccgaac gcgatggtcg ccaccacgat cagccggtcc gaggccatga acgcatcctg
     8641 cacgctgttg cgcacctcgg cgtccagccc ggcatggtag gcctgggcgg caaaaccgcg
     8701 cttgcagagg gcctgcgcga cctcttccgc cgtccgctgc agcgtcacat acaccaccgt
     8761 gggcccggcc gggcgctgct ccacgcgcga tagcagcagc tctagtcgct cctgggcggc
     8821 gcacggcgtc acccgcaggt tgaggttggg ccggtagaag ccggtgtgca ccacgtcatt
     8881 cttggtgatg ccaaactcgc gcgtgatatc cccggccacg gtcggcgtcg cggttgcggt
     8941 gagcgccagc acccgctcca ccttgagctg cctggccaat cgggcaatct tcaggtaatc
     9001 cgggcggaag ttgtggcccc actcgctgat gcagtgggcc tcgtcgatag ccagcaagga
     9061 aattcggcgg ccttccagca ggtgcagaaa gcgttcgttg cccagccgct cgggcgagac
     9121 gtaaagcagg cgcagcgtgc cggaacgaag gccctcgtac accccgcgcg tttcgtccgg
     9181 tccgaggctc gaatccagcc gggccgccgg cacggaccgg gccgtaagga actcgacctg
     9241 atctttcatc agtgcaatca ggggggagat gatgacggtc aggccgtcga agagcagcgc
     9301 gggcaactca tagcacaggc tctttccgcc gccggtcggg aagatcgcca gcacgttgcg
     9361 ccgcgcgagc aggcgttcga tgaccgcctg ctggccggcg cggaagtctt caaagccgaa
     9421 gacacgccgc aacaggtcgc gcgcgctcca cgtttgcgag ccttgagcgg ctgaaacgcc
     9481 ggcgaccggc ggcgccgttt ctgaccgcga gccccgcgac gaaggcggct cgcgacgtgg
     9541 cggcgccggc cggccgccca tcctcctctc tgaccccgtt cctgactttg ccgcgtgctt
     9601 cacgttctgc tccctgcccc tcaagttgcc gatgggtctg acgccgcggc caataatcgc
     9661 gtaagcgaga gccgcctacc gggccacgcg cacccaaaca aaaccctaat atatcgccgg
     9721 cggcgaacag cgtcaagatt aaaaccagcg tggcggcggc cggtcgtggc tgatgtcaaa
     9781 acgtcaagat ggcgcagagc atagcaccgc tcaatcgctg cagaacggcg caagagcttt
     9841 actacaaaag acttgcagaa tgcgaggtca ggaagccgtc cccgccggct ggtccgcttt
     9901 ccgcggccga tgatcgagca gcagccggtc gcgcatcatg cggccgacct tgaatttgac
     9961 agtgcgtttg ctggggacgt gcactttttc catcgttttg gggttctgcg ccactcgcgc
    10021 cgcccgttcg cgcagctcaa acacgccgaa atcccggaat tccaggcgat ttccctgtcc
    10081 caattcgcgg acgatctctt ccagaaactg ttgaatgacg cgtttgacca caatgcgctt
    10141 gtagttagtc tgttcggata tccggtcgac aaggtgtttt ttggtgatcg tcttcatcaa
    10201 cgttcccctg tccgaaccgc cgaggggcgg ccatctccaa
//
LOCUS       DAOSYS010000125        10223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5582_length_10223_cov_5.839201, whole genome shotgun sequence.
ACCESSION   DAOSYS010000125 DAOSYS010000000
VERSION     DAOSYS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5582_length_10223_cov_5.839201"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..812)
                     /locus_tag="PLU99_09020"
     CDS             complement(12..812)
                     /locus_tag="PLU99_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17238.1"
                     /translation="MSQRSIRSPQKRLKYPWLFALRNGELPPIISCYGTEYQLRQVFK
                     HDFFAATGLYAGPAGQVVAKFGRTSDFLSLPLAWAGDLLETREYRVLEQAQDIPGVPR
                     LVGRVGENVLVREFVPGHPLGRREAVSDSFFEELGAILRALHARHIAYVDLNKRQNIL
                     VGDDGRPYLIDFQISLHLPPTGWRRAWPVRRILARFQQADLYHYLKHKRRLRPDLLTP
                     AERARVERLGLWIRLHRLLARPLTHLRRGILARLRTDEAEAVPGSGAK"
     gene            981..1421
                     /locus_tag="PLU99_09025"
     CDS             981..1421
                     /locus_tag="PLU99_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO17239.1"
                     /translation="MFRTKASTYAILATLEIAKRQKEENGGIQAGEIARELNLPAAYA
                     AKVLTQLARANVLRSDRGPRGGFRLMRDPEKVDVLEIVEAVDGSMHAGLEWASRFEMG
                     DGKTRAVDGVFDEVVQQVRSYLRGRTLHDLLGEQKPLPSMAHLS"
     gene            complement(1666..4311)
                     /locus_tag="PLU99_09030"
     CDS             complement(1666..4311)
                     /locus_tag="PLU99_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17240.1"
                     /translation="MSLIEASLPADAAQALSMAPKRRTIFTLAAVVVLAVLVAGLPLV
                     SAPWLPGDEHIFIVNNSAVTGADLTPDTLGQRLALIFQFPPEEDLYQPLPILTYALQW
                     TLTGSSALFFRLADVTLHAINALLLWATLYVLLGRICSAAQRATLLVVTWAATLIWAL
                     HPMLVGAWAADMGRTHLLATTFALSALLLHVRSIEKRRPALFLAATLCLIAAMLSKVL
                     AGWVLVAAALELWLVGWRAMLKSARIYVIAGICALFGAAAYWTSLQSGLIQDASEGLF
                     GDPVSRSALAVWIYFRNLLVPAWLSPWYLPDPLTNWSNPRVWVGLALAVATVAWTVWA
                     WRRPNQRPIAIGWAWFWAQLLPVIGLVGAREAAANDRYMYQPLMGLMLVLSAAVVTLN
                     SNAAQIRFRPKSVTLFAAVIAAVLLLLNQPVVRICRRPVANGQRVVQLYPGDPRALEG
                     LSAQYNFARGHALTADDLAQVPAGQTQAEYFLSQRLLTLRQAAATPGLERFFPRASDL
                     APFHRRVSAAFADLRQYRDALMQAEAALRLEPENYRTWVRLAHAYVGLEDFEQATKAF
                     ERCEQLLPADALTQATHYTNYGRLLLFNLERADLAFPKFRQAVLLKGEVPARYHRELA
                     IADIGLARCEIREGKGERGYELVMQVLNADPQNALAGLVLAEYHFRSEHWQEAAAVYE
                     QLIRAYPVAYRWFDWYYEALRGYQWVSIRTNRLREAALMWDHAVQVQPGRRELLSFRA
                     WSLAIAGESGAEAAINDLLTHESDNQLGCLGRAVVALRNGDIEGAIASVGQARTGLEI
                     PQARAFERTLWAIERLKNEAWPAETVLVEAAITMAAGAREAAERLLESYAGAGADSEW
                     IYLPDVIRSWPETAPAASQPGKNGG"
     gene            4448..5155
                     /locus_tag="PLU99_09035"
     CDS             4448..5155
                     /locus_tag="PLU99_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOO17241.1"
                     /translation="MLPGTRNGSADLARLVEKRLRTWRAAPRPPAPRGAPRPEVYDFV
                     TISRMVGTNGYGLAAALGKRLGWPVFDREILHVMARDDRLRERLYEELDERDVGWIEN
                     MLRWLLEEGIHKERYFHRLSETILALARQGPAIFLGRGADLILPRERGLRVSLVAPLE
                     FRIRNIAVREGLSEPAARKHVTRVQAQRDKFLRTHFGARLENPARHDLVINVAAIPAS
                     TAIELISMTLRLQGVVR"
     gene            5281..5739
                     /locus_tag="PLU99_09040"
     CDS             5281..5739
                     /locus_tag="PLU99_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOO17242.1"
                     /translation="MDEATLASWMRNESARIRDLASELREHVTTIPPRSREPWLKELA
                     LRFDHLKAHLYRMMAIEEEDGYLTPVIEHRPGLTGAVEALRHEHDELRWLTENLAETV
                     RNLKPEDNLILRDTCTRIQVYLGHVQRHEEHENHIVLYTLTEDIGTTESG"
     gene            complement(6025..7044)
                     /locus_tag="PLU99_09045"
     CDS             complement(6025..7044)
                     /locus_tag="PLU99_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOO17243.1"
                     /translation="MKLTHYRQELRPRHVFATSQGSVDYKQTIVVQLEHEGITGLGEV
                     VASALYGQTLEACEDALDSMLPLLGDDLFALESIIARLIARHGDQRAAIAGVDSALHD
                     WIGKRLNISVWRLLGLDRPRKRTTFTIGVADMAAVRVKVDEALAAGYDALKVKVGVEH
                     DHEVLSLIRSRFDGPLLLDANQAWQPDEAVKKIKALTPYKPAMIEQPLRAEDWREMGR
                     LCELGVAPIYADESCERLEDVTRLHGYVDGVNIKFTKCGGIREALKMIRVARGLGMGT
                     MLGCFVSSSLAIAPPLCLASLVDFIDLDGHLLLAEDPFEGITNTGSILELSDRPGLGI
                     EPRRT"
     gene            complement(7041..7361)
                     /locus_tag="PLU99_09050"
     CDS             complement(7041..7361)
                     /locus_tag="PLU99_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17244.1"
                     /translation="MQQPSLNDLLNRRVVLDTQGPLLYIGTLLAWDDRGYWLTEADVH
                     DRDDGHASKEEYTNTARELYQTGARHINRRRVFVERHAVVSVSALDDVVMEGLADGQG
                     PWIE"
     gene            7504..8253
                     /gene="recO"
                     /locus_tag="PLU99_09055"
     CDS             7504..8253
                     /gene="recO"
                     /locus_tag="PLU99_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOO17245.1"
                     /translation="MAIEKDNGFVLRLTEFSETSQIVTLFTERSGQVRLIAKGARRST
                     SKRFSAGLDQLELGEVSYVPSHGDAQLGTLTEWVQRETFPGLRRELPRLYGGLYVAEL
                     AATLTEEYDPHPALFAALKRSLAELAGQAEPLRVLAIYQAELLSAIGYWPNFESCVDC
                     GRVRPSGAAAWFSSTAGGLLCRDCEAHHVEKRRLPGNLLDTTAATGNAQAWFELQHYH
                     LTHVAGRAFKTAGRLFQSLGHGSDGRDRNRC"
     gene            8498..9097
                     /locus_tag="PLU99_09060"
     CDS             8498..9097
                     /locus_tag="PLU99_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO17246.1"
                     /translation="MKRPAVDSEEFRLLDAAAAGDREARRQLFERHRDAAYQVAFRLT
                     GRMEDALDVVQDSFITAFEKLGGFARASGFKTWLLRIATNRALDLLRSRRLRLGVSLE
                     AVEGEGGLPRMVALPLEERAVSSLEQQELGERIRQAVELLPPEQRAVFSLYAAGDMTY
                     GQIADVLEVPIGTVMSRLFHARRRLHELLPDLVPRVTGR"
     gene            9212..9706
                     /locus_tag="PLU99_09065"
     CDS             9212..9706
                     /locus_tag="PLU99_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17247.1"
                     /translation="MEAKNRDLRTALTAIEAGNLDSLAPDEIARLEAFLNASTGAAAA
                     LTDKVPTPEPALQIRAPRPTAASWALVWRQIEARAAVAAARRHVLSGRMWRSALAAAA
                     VCAIAVGLWSLQHATSEPEWPVAWATHIEIDDLQVSEGAKTRVLGTETESSFAVIWVV
                     ESGG"
     gene            9745..10221
                     /locus_tag="PLU99_09070"
     CDS             9745..10221
                     /locus_tag="PLU99_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17248.1"
                     /translation="MNRALIGALCALFLVPGTRLASGKGNAENQNADKIAVEVWTIRA
                     TTTNKIISPELKDLAKKLHKDAEFKKYTGFMLESRATGKTDLGRGYSVELLEQYRATV
                     TPRQREKKNIKLQFEAERQGKPILNTTITAPVGSLVPFGVGPLSNEDFLITAVRAR"
BASE COUNT         1991 a         3090 c         3352 g         1790 t
ORIGIN      
        1 aggaaatcgg attacttggc tccggaaccc ggcaccgctt ccgcctcgtc cgtgcgcaac
       61 cgggctaaga tgccgcggcg cagatgtgtg agcgggcgag ccaggagacg gtgcaggcga
      121 atccaaaggc cgaggcgttc aacgcgggcg cgctccgcgg gcgtgagcag gtccggccgc
      181 agacgccgct tgtgcttcaa gtagtggtag aggtcggcct gttggaagcg agctaggatt
      241 cttcgaacgg gccaggcccg ccgccagccg gtgggcggca aatggaggga tatctgaaag
      301 tcgatcaggt atggccggcc atcatcgccc acgagaatgt tctgccgctt gttgagatcg
      361 acgtacgcga tgtgccgcgc gtgcagcgcc ctcagaatcg cccccagctc ctcgaaaaag
      421 ctgtcggaca ccgcttcgcg gcgccccagc ggatgccctg ggacgaactc ccgcaccagg
      481 acgttttcac caacgcgccc gacgaggcgg ggtacgccgg ggatgtcctg ggcctgctcg
      541 aggacgcggt attcacgggt ttcgagtaag tcgcccgccc aggccagcgg cagggaaaga
      601 aaatcgctcg tccgcccgaa tttggcgact acctgcccgg ccggaccggc gtacaggccg
      661 gtcgcggcga agaaatcgtg cttgaaaact tggcggagtt gatactcggt gccataacaa
      721 gaaattatgg gcggtagctc accgtttcgt aaagcaaaca gccacgggta cttaaggcgc
      781 ttttgcggag agcgaatact cctttggctc atgtccagaa ttataatcgc cgtggggcta
      841 aggtcttatt ggtttttttt gtttacagac acataatatc tttggtaatg cctcggggcc
      901 ggccggggcg agacggatag ggaaggctgt tctcgttttt gcctgcaagt caggctcggc
      961 tactggggaa gtggtgcgcg atgttcagaa ccaaagcatc tacgtacgca attctggcca
     1021 ctcttgagat cgcaaaacgg caaaaggagg aaaacggcgg tattcaggcg ggtgagatag
     1081 cgcgtgagct caatttaccc gcggcttatg ctgcgaaagt gctcacgcag ttggctcgcg
     1141 ccaacgtgct acggtcagat cggggtccgc gtggcggatt ccggctgatg cgcgacccgg
     1201 agaaagtcga cgttctcgaa atagtagagg cggtcgacgg atcaatgcac gccgggctgg
     1261 aatgggccag ccgcttcgag atgggggatg gaaagacccg tgcagtagac ggggtatttg
     1321 acgaggtggt gcagcaagtc cgctcgtatc tgcgcggacg tacgctgcac gatctactgg
     1381 gcgagcagaa accgctcccg agcatggcac atctaagcta ggttatggga ccgatacgtg
     1441 ccgggttgcc cacgctttca aattaaaaaa aaggcgtgcg gcaccccggt gggtattggc
     1501 gcggtctgag ggtgccgcag cgagtggaag caagttcagt tttgaaaagc acttacgaaa
     1561 atcgtaagtt tctgtggcta atgccacatg ctcatcacac ttgaaagtat aacaaacgct
     1621 caggtagttt caaccacccc atgcgacaat ttgtacgaaa atccgtcacc caccattttt
     1681 tcccggctgg gatgccgcgg gagcggtttc tggccaactc ctgataacgt ctggtaaata
     1741 aatccactcc gaatcagcac ccgccccagc ataactttcc agtagccgct ccgcagcctc
     1801 acgcgccccg gccgccatcg tgatcgccgc ttccaccagc accgtctcgg ccggccacgc
     1861 ctcatttttc agccgctcga tggcccaaag cgtgcgctcg aacgctcgcg cttgcggaat
     1921 ttccaagcct gtgcgggcct ggcccacgct tgcgatggcg ccttcgatgt cgccgttgcg
     1981 caaagcgacc accgcgcgcc ccaggcagcc gagttggttg tcgctttcgt gtgtcaacaa
     2041 atcgttgatc gccgcttccg cgccgctttc tccggcgatg gcaagcgacc atgcccgaaa
     2101 agatagcagc tcgcgccgcc ccggttgcac ctgcaccgcg tggtcccaca tcagggccgc
     2161 ttcgcggagc ctgttcgttc gaatactgac ccactggtac ccgcgcagcg cctcgtagta
     2221 ccagtcaaac caccgatacg cgaccgggta cgcccggatc agttgctcgt aaaccgcggc
     2281 cgcttcctgc cagtgctcgc tgcggaagtg gtactccgcc aaaacgagtc cggcgagagc
     2341 gttctgcgga tcggcattca gaacctgcat gactaactcg tacccgcgct cgcccttgcc
     2401 ctctcgaatc tcgcaacgcg ccaggccgat atcggcgatc gcgagttcgc ggtgatagcg
     2461 cgccggcact tcgcccttca acagcaccgc ctgccggaat ttcggaaacg ccaaatcggc
     2521 tctttccagg ttgaacagca gcagccgccc gtaattcgtg tagtgcgtcg cttgtgtgag
     2581 tgcgtcggcc ggcagaagct gctcgcaacg ttcaaacgcc ttcgttgcct gctcgaaatc
     2641 ctccaggcca acgtaagcgt gagccaggcg cacccacgtg cgatagttct caggctcgag
     2701 tcttagcgcc gcttcggcct gcatcagagc gtcgcggtat tgcctgagat ccgcgaaggc
     2761 ggcggaaacc cggcggtgaa aaggcgccag gtccgacgca cgcggaaaaa agcgttccaa
     2821 gccgggcgtt gcggccgcct gcctcaacgt gagcaatcgc tgtgagagaa agtattccgc
     2881 ctgcgtctgg ccggcgggta cctgcgcgag gtcgtcggcg gtgagcgcat ggccgcgggc
     2941 gaagttgtac tgcgccgaca ggccttccaa ggcgcgcgga tcgccgggat agagctgcac
     3001 gactcgctga ccgttcgcca ccggccggcg gcagatacgg accaccggct gattcaacag
     3061 cagcaggacg gcagcgatga ccgcggcgaa gagggtaacc gatttcggcc tgaaccggat
     3121 ttgggcggca ttgctgttaa gcgtcacgac ggcggcgctg agcaccagca tgagccccat
     3181 caggggttgg tacatgtagc ggtcgttggc cgccgcttcg cgcgctccga ccaagccgat
     3241 cacgggcagg agctgcgccc aaaaccacgc ccagccgatg gcgatggggc gctggttcgg
     3301 gcggcgccag gcccatacgg tccatgccac cgtcgccacg gcgagcgcca ggcccaccca
     3361 tacgcgcgga ttgctccagt tggtaagcgg gtccggcaag taccacggcg aaagccaggc
     3421 cggcacgagt agattgcgga aataaatcca taccgccagg gcactgcgcg acactgggtc
     3481 gccgaacagc ccctcggagg cgtcctgaat gaggccggac tgcaagcttg tccagtatgc
     3541 ggccgcaccg aagagcgcgc agatcccggc gatcacgtaa attcgcgccg acttcagcat
     3601 cgcgcgccag ccgacgagcc agagttccaa cgcggccgcg acaaggaccc agccggccag
     3661 gactttcgac aacatcgcgg caatcaggca gagtgtggcc gctagaaaga gcgcggggcg
     3721 gcgcttctct atggagcgaa cgtgcaacag cagtgccgac agcgcgaaag tggtggccag
     3781 caggtgcgtg cgtcccatgt cagcggccca ggcgccgacc agcatcgggt gcagcgccca
     3841 gatcagcgtg gctgcccacg ttacgaccag gagcgtggcg cgctgcgcgg ccgagcagat
     3901 acggcccagc aaaacgtaga gcgttgccca cagcaaaagg gcgttaatgg cgtgcagcgt
     3961 gacgtcggcc aggcggaaga agagggccga gctccccgtc agagtccact gcagggcgta
     4021 tgtaagtatg gggagcggct ggtacaggtc ctcttcgggc ggaaattgga aaatcagcgc
     4081 caagcgttgc cccaacgtgt caggcgtgag gtcggcgccg gtcacggctg agttattcac
     4141 gatgaagatg tgctcgtcac ccggcaacca gggtgcgctg accagcggca gtccggcgac
     4201 gaggactgcc agcacgacca ccgcggccag cgtgaaaatt gttcgccgtt tcggggccat
     4261 cgaaagggcc tgcgcggcgt ccgccgggag agaggcttca atcaaactca tgtgaacgtt
     4321 atggccgggg caagcacgag ccgcaagtgg cgcgagcggc cgggtttcgt tggccgtcgc
     4381 tgactaagat tacaatggtg gcgccaagtg gcgagcggtg gacacattag ggggcttgga
     4441 gagccttatg ctgcccggca cgagaaacgg aagcgcggat ctggcccggc tggtcgaaaa
     4501 gaggctgcga acttggcggg cggcgcccag gccacccgcc cccaggggcg ccccgcggcc
     4561 cgaagtttac gacttcgtga ccatctcgcg catggttggg accaatggtt acggcttggc
     4621 cgcggcttta ggcaagcggc tgggctggcc ggtgttcgat cgcgaaatcc tgcatgtgat
     4681 ggcgcgcgat gaccggctgc gtgagaggct atacgaggag ctggacgagc gcgacgttgg
     4741 ttggatcgag aacatgctgc ggtggctgct cgaggagggc atccataagg agcgctactt
     4801 ccatcggctg agcgagacca tactggcgtt agcccggcag gggccggcga ttttcctggg
     4861 acgcggggcg gatctgatcc tgccgcgcga acggggcttg cgggtcagcc tggttgcccc
     4921 gctcgagttc cgcatccgca acatcgccgt tcgtgaaggt ttgagcgagc ctgcggcgcg
     4981 gaaacacgta acgcgcgtgc aggcacagcg cgacaagttc ttgcgcaccc atttcggtgc
     5041 ccgcctcgag aacccggcgc gacatgacct ggtcatcaac gtggcggcca tccccgcctc
     5101 cacggcaatc gaactcatca gcatgacgct gcgcttgcag ggtgtggtac gataaccgaa
     5161 cggagccggg gcgcgtcgcc ccggagtcgt ccgggaagtg aaagaggcgg ccctttcggg
     5221 tgacgcgcgc actcccggcg aagaggcgac cgaacggccc cgcaaagctg gagtgagcat
     5281 atggacgagg ctaccctggc gagttggatg cgcaacgagt cggccagaat ccgcgatctg
     5341 gccagtgagc tgcgtgaaca tgtaactaca atcccgcccc gaagccgcga gccctggctg
     5401 aaggaattgg ctctccgttt cgaccacctg aaggcccatc tgtaccgcat gatggctatc
     5461 gaggaagaag acggctacct cacgccggtg atcgaacacc gccccggcct gaccggcgcg
     5521 gtcgaggcac tgcggcacga gcacgacgag ctgcgctggc tgacggagaa cctcgcggaa
     5581 accgttcgca acctcaaacc cgaggacaac ctcatcctca gggatacttg cacgcgcatc
     5641 caggtctacc tcggccatgt gcagcgccac gaggaacacg aaaatcacat cgtcctgtat
     5701 acgctgaccg aagatatcgg cactaccgaa tccggctgag cgaggatttg gctaagcgcg
     5761 cgaatttggt tgagcgcgaa tccgaaccca gagcagagcc ggggcgtgtc gccccggatt
     5821 gccgccgaga acctgccatc tgcgcagtaa agggccctcg atccgagctc cgcggcacga
     5881 tccgagccct ttgatccgag cccgaagcgc cagcgagggc aacccgatcc gtgctgggcg
     5941 tgcggccgga gcgattgccc gcgctttgct caaattccgg ctcgacacga gccggctctg
     6001 ctactctgcg cacgggcggc tctgctatgt gcgccgcggc tcaatgccca accccgggcg
     6061 gtcggagagt tcgaggatgc tgccggtgtt ggtgatgcct tcgaacggat cttccgccag
     6121 caacaggtga ccgtccaagt ctatgaaatc caccaggctt gccaaacata ggggtggggc
     6181 gatagcgagg ctgctggaaa cgaagcagcc cagcatggtt cccatgccga ggccgcgcgc
     6241 cacgcggatc atcttcagcg cttcgcgaat gccgccgcac ttcgtgaact taatgttcac
     6301 gccgtctacg tagccgtgca ggcgggtaac gtcttcgagg cgctcgcagc tttcgtccgc
     6361 gtaaatgggg gcgacgccca gctcacacag gcggcccatt tcacgccagt cttccgcgcg
     6421 cagcggctgc tcgatcatgg ccggcttata cggcgtcagg gccttgatct tcttgaccgc
     6481 ctcgtccggc tgccaggcct ggttcgcgtc cagaagcaac ggaccatcga agcgactgcg
     6541 gatcagcgag agcacctcgt gatcgtgctc gacgcccact ttgactttca atgcgtcgta
     6601 gccggcggcc agggcttcgt ccactttgac gcgcactgca gccatgtcgg ccacgccgat
     6661 cgtgaaggtc gtgcgtttgc gcgggcggtc gaggccgagc aggcgccaga cggagatatt
     6721 caggcgtttg ccgatccagt cgtgcagggc ggagtcgacg ccggcgatgg cggcccgctg
     6781 gtcgccgtgc cgtgcaatca ggcgggcgat gatgctctcg agcgcgaaga ggtcgtcgcc
     6841 gagcaatggc agcatcgagt cgagcgcgtc ctcgcaggct tccaacgtct gcccgtataa
     6901 ggccgacgcc acgacctcgc ccaagccggt aatgccctca tgctcgagtt ggacgacgat
     6961 ggtctgcttg taatcgacgc tgccctgcga cgtcgcgaag acgtgccgcg ggcgcagttc
     7021 ctggcgataa tgcgtcagct tcattcgatc cacggtccct ggccgtcggc caagccttcc
     7081 atgacgacat cgtcgagggc cgacacgctt acgaccgcgt gccgctcaac gaatacccgc
     7141 cgccggttaa tgtgccgcgc cccggtttgg tacagctcgc gcgcggtgtt ggtgtattcc
     7201 tccttcgagg cgtgaccatc gtcgcggtcg tgcacgtcgg cttcggtcag ccaataaccg
     7261 cggtcgtccc aagccagcag cgtgccgatg tacagcagcg gcccctgggt atccaggacg
     7321 acacggcggt tgaggaggtc gttcaacgag ggttgttgca tttgtgccgc ctgaacctcc
     7381 gctgcaagcg ttcaggtcac gtgtcggcag cggaaaacgg ggctattgta tccctgccga
     7441 ccggcatctc aaccgccgga cggccaatat gcccgactgc cccgtcgggt ctgagccgcg
     7501 actatggcga tagagaagga caatgggttc gttctaaggc tgacggagtt ctctgaaact
     7561 tcgcagattg tgacgctctt cacggagcgc agcggacagg tgcggctgat cgcgaagggc
     7621 gcgcggcgta gcacgtcgaa gcggttttcg gccggcttgg atcaactgga actgggggag
     7681 gtcagctacg ttcccagtca tggagatgca cagcttggca cgctgaccga gtgggtgcag
     7741 cgcgagacct ttcccggctt gcggcgcgag ctgccgcggc tctatggggg gctgtatgtg
     7801 gcggagctgg cggcgacgtt aaccgaagaa tacgacccgc acccggcgct cttcgccgca
     7861 cttaaacgca gcctcgcaga gctggcgggc caggcggaac cgttgcgcgt tctggcgatc
     7921 tatcaagctg agctgctgag cgcgatcggg tactggccga acttcgagag ctgcgtcgat
     7981 tgcgggcggg tgcggccgag tggggcggcg gcctggttca gctcgaccgc gggcggcctg
     8041 ctatgccggg actgcgaggc acaccacgtc gaaaagcgcc ggctgcctgg caacctgctc
     8101 gacacgacgg cggcgacggg caacgctcaa gcgtggttcg agctccagca ttatcacctg
     8161 acgcatgtgg ccgggcgggc attcaagacg gccggacgtc ttttccagag tctgggccat
     8221 ggcagcgacg ggcgggatcg gaataggtgc tagttcgaag aaggcggcgc cgggccacct
     8281 cggcccggcg ccctcacgcg cttcgtgcac cgaccgggga taaggtcata gactacacta
     8341 cgcgcgtttg gttcttggaa cggacgcgcc gccggtcctg gagtttcgat ccgggttctg
     8401 acgaacaagc caactagatt attcatcgac ccttcagtcg agccacagat ttttgctatg
     8461 ctgcccggag ggggtgagcg gtattgcggc agaaccattg aaacgcccag ccgttgacag
     8521 cgaggaattt cgtcttttgg atgccgcggc agcaggcgac cgggaggcgc gccgccagtt
     8581 attcgagcgc caccgcgacg cggcctatca ggtggctttc aggctcacgg ggcgcatgga
     8641 agacgcgctg gacgtagtgc aggacagctt cataacggca ttcgagaagc tgggcgggtt
     8701 cgcacgtgca tcgggattca agacgtggtt actccggatt gcgacaaacc gggcactgga
     8761 tttgctgcgc tcgcggaggc tcagactcgg cgtgtccttg gaagcggtcg agggtgaggg
     8821 cggtttgccg cggatggttg ctctgccgct tgaagagcgc gcggtgtcga gcctggaaca
     8881 gcaggaattg ggggagcgga tccggcaggc cgtagagttg ctgccgccgg agcagcgagc
     8941 cgtgttttcg ctgtacgccg cgggcgatat gacctatggg cagattgccg acgtattgga
     9001 ggttccgatt gggacggtca tgagcaggtt atttcatgcg cgccggcgcc tgcacgagct
     9061 actgcccgac ctcgtgccgc gcgtgacagg ccgataaata ctggccgacc actcagtgaa
     9121 gatttacaga atgatctggc gggtcgaaac gtcaaaacca tgaatggcgg cgtgcatttc
     9181 gggcccaagc gcgctggctg aggatccagt catggaggcg aaaaatcgcg atttgcggac
     9241 cgcgttgacg gcgattgagg ccggcaacct cgattcactc gcgcccgatg agatcgcacg
     9301 cctggaggcc ttcctgaacg ccagcaccgg agcggcggcg gcgttaaccg ataaggtgcc
     9361 cacgccggag cccgctttgc agattcgtgc tccgcgtccg acggctgcct cgtgggcgct
     9421 agtttggcgt cagatcgagg ccagggcagc ggttgcggcc gctcggcggc acgttttgag
     9481 tgggcgcatg tggcgatccg ccctggctgc ggccgcagtc tgtgcaatag ccgtgggtct
     9541 gtggtccctg cagcacgcga cgtcggagcc ggaatggccg gtagcgtggg ccacgcacat
     9601 tgagattgac gatctgcaag tctccgaggg cgctaagacg cgggtgttgg gcacggagac
     9661 tgaaagctcg ttcgccgtga tttgggttgt ggaaagcggc ggttagccgg tcgccggcac
     9721 gcccgggtga aagaggagtc aaaaatgaat cgcgccttga tcggggcgct ttgcgcacta
     9781 ttcctcgtac cgggcacgcg cctggcaagc gggaaaggca acgccgagaa ccaaaacgcg
     9841 gacaaaatcg ccgtcgaagt gtggaccatc cgcgccacaa ccacaaacaa gatcatctcg
     9901 cccgagttga aggacttggc caagaagctt cacaaagacg ccgagttcaa aaaatacacc
     9961 gggttcatgc tggagagccg cgccacgggc aagacggatc tgggtcgcgg ctacagcgtc
    10021 gaactgctcg agcagtaccg ggcaaccgtg acgccgcggc agcgcgagaa aaaaaatata
    10081 aaactgcagt tcgaagccga gcgacagggc aagccaatcc tcaacacgac catcacagcg
    10141 cccgtcggct cgttagtgcc attcggcgtg gggccgctga gcaatgagga ttttctgata
    10201 acggccgtgc gggcgcgctg aaa
//
LOCUS       DAOSYS010000126        10205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5594_length_10205_cov_5.001379, whole genome shotgun sequence.
ACCESSION   DAOSYS010000126 DAOSYS010000000
VERSION     DAOSYS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5594_length_10205_cov_5.001379"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLU99_09075"
     CDS             complement(<1..284)
                     /locus_tag="PLU99_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17249.1"
                     /translation="MLVPVCNVLVGVGIGFCLGFTRGATGQSVEDVPEQIEAFVSSPG
                     GMLLFLFVGQAVFALVLWLATLRQRGARRIWLGMLPARISRSEYPLLVLGT"
     gene            complement(413..1501)
                     /locus_tag="PLU99_09080"
     CDS             complement(413..1501)
                     /locus_tag="PLU99_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO17250.1"
                     /translation="MNHPVGQLASLLAALAWAGALVLFKRSGERVPPIPLNLFKNAVG
                     ITLLALTLLVTSGLDYWCGLFGGACDAALFLRLSLYDYLILVASGIIGIAVADTFFFH
                     ALNLIGVGLVSIVDCCYSPSVLLFAWLLLGEDLSPYHYLGGGLILLGVLIASGHAPPP
                     GHTRGQILLGICLGILAISSMALSIVIATPVLKHVPLVWATTVRLIGGSAALLAVTPL
                     LPGAATHWAIFKPARVWYQAIPAAFLGTYLSLLAWIAGFKYTYASVAAVLNQTSIIFA
                     LILASIFLRERFGTRKLLAVELALGGVLIITFPDHCMRLAERPGLAISLAAGLIAAPV
                     WYQWRLKRLAPAVSPAVPPAPLEKERIS"
     gene            1734..2249
                     /locus_tag="PLU99_09085"
     CDS             1734..2249
                     /locus_tag="PLU99_09085"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO17251.1"
                     /translation="MPGILCEHCTAACCRYVALPIETPTERSDFDDIRWYLLHENISV
                     FVEDGDWYISMQTSCRQIAADGRCMIYARRPRICRKYTTENCDYHSGDYGWEAHFTCP
                     EHLEEYLRARSLSKTSKSSKNGDNRTSMNNQKTKKSYKATDGTAGGRAVNRPAGRARP
                     RRASSAPEGRP"
     gene            2246..3553
                     /gene="hisS"
                     /locus_tag="PLU99_09090"
     CDS             2246..3553
                     /gene="hisS"
                     /locus_tag="PLU99_09090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOO17252.1"
                     /translation="MKIQVPPGMRDFYPPEMRLQNWLFQHWREVSRAFGCEEYEGPIF
                     EYLDLYTLKSGAGIVSELFNFEDRGERRFAIRPEMTPTLARMVAARANALPRPIKWFS
                     TPRMCRAEKPQRGRLREFFQWNVDILGVDDVLADAEVIAVAVEFVRRIGLSERDVVMR
                     VSSRPIAASVLAGMGINSDEEANRAFGLIDRYEKLGRDEFRRQWQEFAPAVDCDQLLT
                     HLEQTDLQHSLSLAEAAGPAGVAAARQFEELWQRLRQFQVDSYCAFDLKVVRGLAYYT
                     GPVFELSARQGGLRALLGGGRYDNLTQLLDGPRVSGVGFGMGDAPVLELLTELGKLPA
                     LSTGLDVFVIDAGPELFPKAIELATQLRRAGFACDFSYKRQAIGKQFAQAEKRGARLA
                     IVVGAELAESGQLTVKDMRTGQQQAVAAAELLADPLSTLGAMR"
     gene            complement(3816..5162)
                     /locus_tag="PLU99_09095"
     CDS             complement(3816..5162)
                     /locus_tag="PLU99_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011045570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17253.1"
                     /translation="MQVSLLNRYRKARAGACCVLVVTLIGLLGVNGCPLDFQSPVFDD
                     GPVQASIDVLLNGKQIFRHDTFGSEAFWGGELLLHEAIAGEANGGVGPGLSPRAALDL
                     GLKVDRNALPAELQTALANNEVDLDDPAVTVELLRLDAVVGIKGFFNDQQQLSAVGIH
                     CALCHSAVDDSFSPGIGNRLDGWANRDLNIGAIVALAPDLTPFAEILSVSVDTVRTVL
                     NSWGPGKFDAELILDGKAFQPDGRSAATLIPPAFGMAGINLHTWTGFGSVPYWNAFVA
                     VLEMHGQGTFFDERLQNADQFPVAAANGFFDVRPAEDLVTSKLPDLHFYQLALDAPRP
                     PAGSFDEQAAQRGQAVFNNFGCARCHVPPLFTEPGFNMHLPEEIGIDDFQANRSPEFR
                     YRTSPLRGLWTHTKGGFFHDGRFPTLQAVIDHYDDFFNLGLSAEQKADLEQYLLSL"
     gene            complement(5695..6129)
                     /locus_tag="PLU99_09100"
     CDS             complement(5695..6129)
                     /locus_tag="PLU99_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17254.1"
                     /translation="MHTPETKPIETADARSSDAIPAGSGHAAPVQVASIHSAPLDGKS
                     LAIGVLSVTAAVFLTAFLLLTALPKPAHAIGMVDAGGDYKMLTQQISSSQEGIVVIDL
                     VAQRMAIYGFDYSRRQLVPLSGFELKELKKSLPAKPHPTPGP"
     gene            complement(6133..6420)
                     /locus_tag="PLU99_09105"
     CDS             complement(6133..6420)
                     /locus_tag="PLU99_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17255.1"
                     /translation="MPKNVWLAALICANVVLLVGIVLVTCSLPAAHAQGVGAGSDLLA
                     VSGEIQDKYDALYLLDTREHTLHVFMWDKSTRQLHYTDWRDLDRDFRTSRD"
     gene            complement(6437..6661)
                     /locus_tag="PLU99_09110"
     CDS             complement(6437..6661)
                     /locus_tag="PLU99_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17256.1"
                     /translation="MFVRRFWTRLALLLILTLPVGIANAADDDDDRGPWYDVPVKPSR
                     TNWVPWVVASAFIVGCVGVAMKNPHRTHLD"
     gene            complement(6758..8329)
                     /locus_tag="PLU99_09115"
     CDS             complement(6758..8329)
                     /locus_tag="PLU99_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO17257.1"
                     /translation="MSTADEHHSDAAPVATEQNRPATPAADSYRGGALVVLLRLHWFV
                     RLRWIFFGVAVAVLAAERVIFPDVRRPVQLAAAVAGVGVLNVIWTALSRALRKRFAEP
                     GEAVEGDVRSARLFANAQVASDLVLLTAILHFTGGVENPMSAFYLFHVTIGALLLTRG
                     QAFLQCCLAIVLYTGMAVAEWSGSLPHFALSPQVAGLDLYRQPAYVALAVIVVACAIL
                     GVFYFAFRIATLLDQHENELKRANAALTQSRQAIADLQARRARFMQTAAHQLKSPLAI
                     AQTLAALIRDGLVTELPEIQNTCVKIIRRCQEGMAQVSELLTLARVQDADPDRHRVSR
                     TDARAVATEVYNRFRPLAESKQIELTCWMPPAGDLIVRVDPRDLRDCISNLLDNAIKY
                     TNGPGRVRLILTVRHVRGRATSLGIHVTDTGIGIDPKLLRSEHLGEEPVFEAFSRGPN
                     ALAAGLPGTGLGLSIVREIVEQAGGRIWVMSRLGIGSSFTVTLPLVDGGPGEPLVRNT
                     RSTDVVLETDGPSHE"
     gene            complement(8390..8953)
                     /locus_tag="PLU99_09120"
     CDS             complement(8390..8953)
                     /locus_tag="PLU99_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOO17258.1"
                     /translation="MIVGLLSDTHGRAELTAFALDVLRQAGAQVFIHCGDVGGETVLD
                     QLATVQAHFVWGNTDEPDTALIAYVRSLGLTPPASIPLRLNISNKAISVFHGHEPEFA
                     RLTRLADGVDPGPFKRQAGADYVFFGHRHVPYDRQCGTVRIINPGAIHRAEVRTVATV
                     DLKHDLLRFWLVDPERPAELPPREWAG"
     gene            complement(9047..9451)
                     /locus_tag="PLU99_09125"
     CDS             complement(9047..9451)
                     /locus_tag="PLU99_09125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO17259.1"
                     /translation="MLAIGDIHVYVSDFATALDFWAGGLQLEVAEKEESEHGAFARLD
                     FPDGGPSLVLIAPVEPWDSESPEPGTAPGFSFDLTTTDFDGTLARLLERNGRQIGETE
                     TYNDLRLATVADPEGNTFELVEVPDELVDVPE"
BASE COUNT         2026 a         3117 c         3291 g         1771 t
ORIGIN      
        1 gtccccagca cgagcagtgg gtactcggag cgggagatgc gggcgggcaa catgccgagc
       61 cagatgcggc gcgccccacg ctgacgaagc gtggccagcc acaagacgag cgcgaacacg
      121 gcttgcccga cgaacaggaa gagcagcata ccgccggggc tggagacgaa ggcctcaatt
      181 tgttccggca cgtcctccac gctctggccg gtggccccgc gagtaaagcc caggcagaag
      241 ccgatgccaa cgcccaccag gacgttgcac acggggacca gcaacagggc ggctgcgaat
      301 tgcagcagga ggggtcacac acgcggcgcg cccgaacgag gggcagagac gggaacggcg
      361 gccgcgggcg ccagcgcgtg tggcgcaacc ggccgggcct gcggaacgtc gctcatgaaa
      421 ttctctcctt ctcaagtggg gcgggcggta ctgcgggaga gacggcaggc gcaagccgtt
      481 tcagccgcca ttgataccag accggtgccg caatcaggcc cgccgcgagg ctgatcgcca
      541 gcccaggccg ttcagcgagc cgcatgcaat ggtcgggaaa cgtgatgatg agcacgccgc
      601 ccaaggcgag ttcgaccgcc agcagctttc tggttccgaa gcgctcacgg agaaagatgc
      661 tggcgaggat cagagcgaag atgatcgaag tctggttcag gacggccgcg actgaggcgt
      721 acgtgtactt aaaacccgcg atccaggcga gcagcgacag gtacgttccg aggaaagcgg
      781 ccggaatcgc ctgataccag acgcgagccg gtttgaagat tgcccaatgg gtcgcggcgc
      841 ccggtagaag cggcgttacg gccagcaacg cggcgctgcc gccgatcagg cgaacggtgg
      901 ttgcccagac cagcggcaca tgtttcagaa cgggcgtggc tatgacgata ctgagcgcca
      961 tcgagctgat ggccagtatg cccaggcaga tgcccaacag aatctgcccg cgagtgtggc
     1021 cgggcggtgg ggcgtgcccg gaagcaatca aaacccccag caaaatcagc ccaccgccga
     1081 ggtagtgata gggggaaagg tcctcgccca gcagcaacca ggcgaacagc agcacgcttg
     1141 ggctatagca gcaatcgacg attgagacga gtccgacgcc gatcaggttc agagcgtgga
     1201 agaaaaacgt gtcggccacg gcgataccga tgattccgct ggcaaccagg atcagatagt
     1261 cgtacaaact caggcgaaga aacaaggccg catcgcatgc tccgccgaac aggccgcacc
     1321 aatagtcgag cccgctggtg acgagcagcg tgagtgccag gagcgtgatt ccgacggcgt
     1381 ttttgaaaag gttgagcggg atcgggggga cgcgttcgcc gctgcgtttg aacagcacca
     1441 gagctccggc ccaggccagg gccgcgagca acgaggcaag ttgacctacc gggtggttca
     1501 tgggctgctg attccgatcc gggcccccga gttcaaggcg tgcgggccaa cgggcccgcg
     1561 cggcggcgtt ggggcagcgc ggtagcataa gggcggcggc gcctggtggg aacgcccgtg
     1621 ggaagcgcaa acgcgggcgg tttgccaacc gcccgcgaac gactatcgta ctgccgccgg
     1681 tgcgcggacc gaccggttcg ggtgcggcgt gcggccggcg atgtgaggta tttatgcccg
     1741 ggattttgtg cgagcactgc acagcggcgt gctgccgcta cgtggcgctg ccgattgaga
     1801 ccccgacgga gcggtcggat ttcgacgaca tccgctggta cctgctgcat gagaacattt
     1861 cggtcttcgt cgaagacggc gactggtaca tcagcatgca gacgagctgc cggcagatcg
     1921 cggcggacgg gcgctgcatg atttacgccc ggcggccgcg catctgccgc aagtacacga
     1981 ccgagaactg cgattatcac tcgggcgatt acggctggga ggcgcacttc acctgtcccg
     2041 agcacctgga ggagtacctt cgtgcccgct ctctctccaa aacctcgaaa tcctcgaaaa
     2101 atggtgataa ccggacttcc atgaataacc agaaaacaaa gaaatcctat aaagccacgg
     2161 acgggacggc cggtggtagg gcggtcaacc ggcccgcggg gcgcgctcgg ccgcgccggg
     2221 cctcctcggc cccggagggg cggccatgaa aattcaagtt ccgccgggaa tgcgcgactt
     2281 ctacccgccc gaaatgcggc tgcagaactg gctgttccag cactggcgcg aagtgtcgcg
     2341 agctttcggc tgcgaggagt acgaggggcc gatcttcgaa tacctggatt tgtacacgct
     2401 caagagcggc gctggaatcg tatcggagct gttcaatttc gaggatcgcg gcgagcgccg
     2461 ttttgcaatc cgccccgaga tgacgcccac gctggcgcgt atggtcgccg cgcgggccaa
     2521 cgcgctgcca cgccctatca agtggttcag cacgccgcgc atgtgccgtg ccgaaaagcc
     2581 gcagcgcggc cggctgcgcg agttcttcca gtggaacgtc gacatcctgg gcgtggatga
     2641 cgtcctggcc gacgccgagg tgatcgcagt cgcggtcgag ttcgtgcggc gcatcgggct
     2701 gagcgagcgc gacgtggtca tgcgcgtcag cagccggccg attgcggcat ccgtgctggc
     2761 gggcatgggc ataaattccg acgaagaggc caaccgggcg ttcgggctca tcgaccggta
     2821 cgagaagctg ggccgcgatg agtttcggcg gcagtggcag gagttcgcgc cggccgtaga
     2881 ttgcgaccaa ctcttaacgc accttgagca gacagacctg cagcatagtt tgagcctggc
     2941 cgaggcggcc ggcccggcgg gtgtggcggc cgcacgacaa ttcgaggagt tgtggcaacg
     3001 cttgcggcaa ttccaggtgg attcttactg cgcgtttgat ctgaaggtgg tccgcggttt
     3061 agcttattac acggggcccg tttttgagct gagtgcgcgg cagggggggc tacgcgcgct
     3121 gttgggcggc gggcgctacg ataatctgac gcagctcctg gacgggccgc gcgtgtcggg
     3181 cgtcgggttc ggcatgggcg atgcccccgt gctggagctg ctgaccgaat tgggcaagtt
     3241 gccggcgctt tcaacgggtt tggatgtttt cgtgatcgac gccgggccgg agctgttccc
     3301 gaaggcaatt gagcttgcca cacagcttcg ccgcgccggg ttcgcctgcg atttttctta
     3361 caagcggcag gccatcggca agcaattcgc gcaggccgag aagcgcggcg cgcggctggc
     3421 catcgtagtc ggcgccgagc tggccgagtc agggcaattg acggtgaagg acatgcgaac
     3481 cgggcagcag caggctgtcg cagccgccga gttgctggcc gacccgttga gcactctggg
     3541 agcgatgagg tagggcgggg cgacggcttg ggtgccatgc ccaagccgcg tagcggcgcg
     3601 ggcatgccca cgtgttcgcc cccgtatgcc gccgcagacc aatggcatgc cggcgcaacg
     3661 gcactcttcc gaccatgggc atgcccgcgc ttagagcttg ggcatggcac ccgcgcttca
     3721 aactctcgat tgcccgatcc ctcttgatcc ctcgatccct tgatcccttg atccctatgc
     3781 gcttttggct ggcacccgcg cctggcgccc acaccttaga gcgacagcag gtattgctcc
     3841 agatcggctt tttgctccgc cgacaggcca agattgaaga agtcgtcata gtggtcgatc
     3901 acggcctgaa gcgtggggaa gcggccatcg tggaaaaatc cgcctttcgt gtgcgtccag
     3961 agtccccgca gaggcgaagt gcggtagcgg aactccgggc tgcggttggc ctgaaagtca
     4021 tcaatcccga tctcttccgg caggtgcatg ttgaagcccg gctcggtaaa cagcggcggc
     4081 acgtggcagc gcgcgcagcc gaagttgttg aaaacggctt gtccacgctg ggcggcttgc
     4141 tcgtcgaaac tgccagccgg cggacgcggc gcgtccagcg ccaactggta gaaatgcagg
     4201 tcgggcagct tggatgtcac caaatcttcc gccgggcgaa catcgaaaaa cccgttggca
     4261 gcggccaccg ggaattgatc ggcgttctgg agccgttcgt cgaaaaacgt accctgaccg
     4321 tgcatttcaa gcacggccac gaaggcgttc cagtacggca cgctgccgaa gccggtccag
     4381 gtgtgcaggt tgatgcccgc catgccgaaa gctggtggaa tgagggtcgc ggcggaacgg
     4441 ccgtccggct gaaaggcttt gccatcgaga atcagctccg cgtcgaattt gccgggtccc
     4501 caactgttca gcacggtccg gacggtgtcg actgagacgg acaggatttc ggcaaagggc
     4561 gtcaggtccg gcgcgagtgc cacgatggcg ccgatgttga ggtcgcggtt ggcccagccg
     4621 tccagccgat tgccgatgcc ggggctgaag gaatcatcga ccgcggagtg gcacagcgcg
     4681 cagtgtatgc caacagcgct caattgctgc tggtcattga agaagccttt gatgcccacg
     4741 accgcgtcca gtcgcaacag ctcgaccgtc accgcgggat cgtcgaggtc cacctcgttg
     4801 ttggccaagg cggtttgcag ctcagccggc agggcgttgc gatccacctt caaccccaag
     4861 tccaaggccg cacgcgggct taagccgggg ccgacgccgc cattggcctc gcccgcaatc
     4921 gcctcgtgca agagcagttc gccgccccaa aacgcttcgc tgccgaaggt gtcatgccgg
     4981 aaaatctgtt tgccattcag caacacgtcg attgacgcct gaacgggtcc atcgtcgaag
     5041 accggcgact ggaaatccag cgggcaacca tttactccaa gcaggccgat gagggttacg
     5101 accagcacgc agcaggcgcc ggcccgcgct tttcgatacc ggttgagcaa tgagacttgc
     5161 atgttttcag ctcctttcgg cgtagagccg gttatcgccg ccgcgaccga caacggcgac
     5221 cgacagcggt ttcccgtgac cgacaacggt tttacgtgac cgatagcggt ttaaagtgcg
     5281 tggtggccgg agcgagattt tccaacaaca tcacgaacgt cgcctggtgc agcgaccatg
     5341 agtacgcgtt tcgcgtccgc gcccgaacac acgcttggcc atagtccgcg ttgcagtgac
     5401 actgaatgct acgcgcccga ggcattgcgc agggagaatt ccaagtaatc cgcgcgcccg
     5461 gcgcggaatg cgatgtgata gtgcgatgcg ataagcacga tgatgttcgg aggaaagatt
     5521 gggcgttcgg aggaaaggtt gggcgtgggg acgaatgacg cagtttggcg acttgccggc
     5581 tattttgtgg gggctacacc caaggtccta taatatatat tatgttgcgt tcgccgcgcc
     5641 gcagcaaaca gtttccgctc aaacccactt ccccggccgt ttaagcccaa aatctcacgg
     5701 acccggcgtt ggatgtggct tggccggcaa cgatttcttg agctccttca gctcgaaccc
     5761 gctaagcggc acgagttgcc ggcgactgta atcgaaaccg tatatggcca tgcgctgcgc
     5821 aaccagatcg atgaccacga tgccttcctg cgaactggaa atctgctgcg tcagcatctt
     5881 gtagtcgccg cccgcatcga ccatcccgat ggcgtgggcc ggcttgggca gcgctgtcag
     5941 gagcaggaac gctgtcagga agactgccgc ggtgacgctg agaacaccga tcgcaagcga
     6001 ttttccgtcg agcggcgcgg aatgaatcga ggcaacctgt accggcgccg cgtggccgga
     6061 gcctgccggg atcgcatcgc tcgaacgcgc gtcagccgtt tcgattggct ttgtttctgg
     6121 agtgtgcatg tcctaatccc ggctagtgcg aaaatcgcga tccagatcgc gccagtccgt
     6181 gtaatgcaac tgacgcgtgc tcttgtccca catgaagacg tgcaacgtgt gttcgcgtgt
     6241 gtctaacaga tacagggcat cgtatttgtc ctgaatttcg cccgaaaccg cgagcagatc
     6301 gctcccggcg ccaacccctt gggcatgcgc ggccggcaat gagcacgtga ccaggactat
     6361 gcccacaagc aacacaacat tggcgcagat aagggccgcc agccagacgt tcttgggcat
     6421 caatcgagct ccagggtcaa tccaggtgcg tccgatgcgg gtttttcatg gccaccccga
     6481 cgcagccgac gataaaggcc gatgcgacga cccagggcac ccagttcgtg cgggacggtt
     6541 tgacaggaac gtcgtaccag gggcccctat cgtcgtcgtc gtctgccgcg ttcgcgatgc
     6601 cgacaggcag agtgaggatc agcaacagag ccagacgcgt ccaaaatctt cgtacgaaca
     6661 taaagcgctc caacccacac atcgtagctc gcggtatgcg gctgaacaag tgggctgtga
     6721 gctggccggg ctggtcaaag cgcctgggtg cgaaaactca ctcgtggctt ggcccgtccg
     6781 tttcgagcac gacgtctgtg ctgcgtgtat tacgtaccag cggttcgcct gggccgccgt
     6841 ccactagcgg aagcgtcacg gtaaagctcg agccgattcc caaacgcgac atgacccaga
     6901 ttcgcccgcc agcctgttcg acgatttcgc gcacgatcga aaggccgagc ccggtccccg
     6961 gcaggccggc cgccagcgca ttaggccccc tgctgaacgc ttcgaagacg ggttcctcgc
     7021 ccagatgctc ggagcgtagc agcttcgggt cgatgccgat gcccgtgtcg gtcacgtgga
     7081 tgccaagtga ggtggcccgg ccacgcacgt gacgaaccgt gagaatcaga cgcacgcgcc
     7141 ccgggccgtt ggtgtacttg atggcattgt cgagcaagtt gctgatgcaa tcgcgcagat
     7201 cgcgcggatc cacgcggacg atcaagtcgc cggccggcgg catccagcag gtcagctcaa
     7261 tctgcttact ttccgccagc ggacgaaagc ggttgtaaac ttccgtagcg acggcgcgtg
     7321 catcggtgcg gctgacgcga tggcggtcag gatcggcgtc ttggacgcgg gccagcgtaa
     7381 gcagctcgga aacctgggcc atgccctcct ggcagcggcg gatgattttg acgcaagtgt
     7441 tctgaatctc cggcagttcg gtaaccagcc cgtcgcggat cagggcggcc agcgtctgcg
     7501 cgattgcaag cggggatttc aattgatggg ccgcggtctg catgaagcgc gcccggcgag
     7561 cctgaagatc tgcgatcgcc tgccgcgatt gcgtgagggc tgcgttcgca cgcttcagct
     7621 cgttttcgtg ctggtcgagc agcgtggcaa ttcggaacgc aaaatagaac acccccaaga
     7681 tggcacaagc gaccacaata accgccagag cgacatacgc cggttgccga tacaaatcga
     7741 gcccggccac ctggggtgat agcgcgaaat gtggcaggct gccgctccac tccgcaacgg
     7801 ccatgcccgt gtaaagcaca atggccaaac agcactgtag aaacgcctgc cccctcgtga
     7861 gcagcagcgc gccgatagtc acgtgaaaca ggtaaaaggc ggacattggg ttttcaacgc
     7921 cgccggtgaa atgcaggatg gccgtcagca agacgaggtc ggaggccacc tgggcgttcg
     7981 cgaacagccg ggcgctccga acgtcgccct caacggcctc gcccggctcg gcgaaacgct
     8041 tgcgcaaagc gcgagagagc gccgtccata tcacgttcag gacgccaacg cccgccacgg
     8101 ccgctgccaa ctgcaccggc cgccgcacgt cagggaagat cacgcgttca gccgcgagga
     8161 ccgctactgc gacgccgaag aaaatccagc gcagccggac gaaccagtgc aaacgcagca
     8221 ggactaccag tgccccaccc cggtacgagt cggccgcggg agtcgccggc cgattttgtt
     8281 cggtggcgac cggagcggcg tcagaatggt gttcgtcggc cgtactcacg tcggcaactt
     8341 agctggccgg tgagaagcgg gcaataccga tgcgcgtggg caagacgtct cagccggccc
     8401 attcgcgggg cggcaactcg gccgggcgct cggggtctac gagccaaaaa cggagcaggt
     8461 cgtgcttcaa atcaaccgtt gccacggtgc gaacctcggc acggtggatt gcgccggggt
     8521 tgatgatccg gactgttcca cactgccggt cataaggcac atggcgatgt ccgaagaata
     8581 cgtaatccgc tcccgcctgg cgcttgaatg ggcccgggtc gacgccgtcg gccaggcgcg
     8641 tcagccgggc gaactctggc tcgtgtccgt gaaagacgga gatcgcctta ttgctgatgt
     8701 ttaagcgtaa cgggatgctg gccggcggcg tcagcccgag gctccgcaca taggcaatta
     8761 atgccgtatc aggctcatcg gtgttgcccc agacgaagtg cgcttgcacc gtcgcgagtt
     8821 gatccaggac cgtttcgccc ccgacgtcgc cacagtggat gaagacctgc gcgccggctt
     8881 ggcggagaac atccagggcg aaggcggtaa gttcggcacg accgtgggtg tcggacagaa
     8941 ggcccacaat cacttttttc ttctccgccc ctacgccctt gagcgtgggg taaaggggat
     9001 taaaggaaca atgcctcgac tccggcccga catgggtaag ctgcgcttac tcgggcacgt
     9061 cgactaactc gtcgggcacc tcgactaact cgaaagtatt gccctcgggg tcggccactg
     9121 tggccaggcg caggtcgttg tacgtctcgg tttcgccgat ttgccggcca tttctttcca
     9181 gaagcctcgc cagcgttccg tcgaagtccg tcgtggtaag gtcgaagctg aagcccggag
     9241 ccgtgccggg ctccggactc tccgaatccc acggttcgac cggggcgatc agcaccagtg
     9301 aagggccacc gtcaggaaaa tcaagccggg caaacgcccc atgctcgctt tcttctttct
     9361 cggcgacttc caattgcagg ccgccggccc agaaatccag ggccgtcgca aaatcgctca
     9421 cgtacacatg aatatcgccg attgccagca tggaaatgct ccaaatccgt tgtttttctg
     9481 cgaggttgct cctcgccgag ccggcgcgcc agcgccagcg gatagagtac tgcgcaattc
     9541 gatttggcac catcgggtgt cgccaccctt gaagctgacg gggcggcggc caggccgacg
     9601 agccgcattc gtgggggttc ctgctccaag ggcgggttcg gtcacaaaca tgagcagccc
     9661 gcagaaagtc gcacacctcg agcttgggcg cggatcgagc gccatgaggt gagtttaggt
     9721 tgtgcggtcc aagcggacaa gaaaggtccg ccgcggttgc cgaaaaccca ctccggcgaa
     9781 cgattacgcg aacgcgtggg ccaagccggc ggatgcgcct cacgcgctcg accaggcccc
     9841 gcaaactgaa tccgccgaca ccgggtggcc ccccctaccg atctcaacct gcgtgaaaac
     9901 gcctccgcca aactgctgga agtaactccg acatgcgttc gccttctgcc gccgccgccc
     9961 gtcgaatgcc tacagcggcg gctccgataa tcgtctatct acagatgtgg acttacggct
    10021 ctaccggcag acctgtctcg tccattctca tcctgcgaat ctcatgctga aaacagacta
    10081 ggcgaaactt ccagtcgatg cacaccgtgt aatgacactc ggcacgcgca ttcgcacctc
    10141 gcttacaagg gcttcacgcc gaattcagtc acgtttcaga agagcggact aagtttgatt
    10201 gtatt
//
LOCUS       DAOSYS010000127        10201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5597_length_10201_cov_4.866450, whole genome shotgun sequence.
ACCESSION   DAOSYS010000127 DAOSYS010000000
VERSION     DAOSYS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10201
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_10201_cov_4.866450"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2200)
                     /locus_tag="PLU99_09130"
     CDS             complement(<1..2200)
                     /locus_tag="PLU99_09130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HOO17260.1"
                     /translation="MEKTRRRRSSAEDTPSLFEAVTPPKLRPIPVGTVSQAVYLAETT
                     ATDGENGRPNRRPATKLRQTRFQPERSKAGKVESKAAKKESKASRRESKESTEPIQPA
                     KSAAAPALAKSAAAPESAPANAAPAPAPEPPAAPADADNTPPAVRAPRSSTVTAETLA
                     NKQRDISVSEFFAKNRHLLGFDNKRKALLTTVKEAVDNSLDACEEAHILPEIHIHIDQ
                     LAEDRFRVTVRDNGPGIIKSQIANIFGKLLYGSKFHRLKMSRGQQGIGISAAGMYGLL
                     TTGKSVHIISRTSPKKPAHYLEIQMDTARNRADVIKDEEIPDIAWERGTEVVIELVAT
                     YAKGRQSVDEYVEQTAIANPHALIHYRAPTGEHRDFPRGATELPAETCEIKPHPHGIE
                     LGVLMKMLKDARPDRKGERTPSLSGFLQREFSRVTPRVAAAICEHAGQSPKARPAQIG
                     THDVERLYQAMQAVKIMAPPTNCLAPIGVRNLLAGLLKEVKAEFYTATTRDPAVYRGN
                     PFQIEIALAYGGSLPADQPARVIRFANRVPLLYQQSACAAFKSVLDVDWRNYGLSQPG
                     NSLPVGPLVVMVHMASVWVPFTSESKEAIADYDEIRKEMKLALQECGRKLAAYLRRRR
                     RQRMEADRRSIFGRYIPDVAEALANLTGLSEDRLVKQLSTIAKMRTAEADVQLDEDGR
                     PIAPATAGGAKAGAAEAAGPASAAGGRKPSPERKRAGKAHSGKAKAAMEPA"
     gene            complement(2419..4020)
                     /locus_tag="PLU99_09135"
     CDS             complement(2419..4020)
                     /locus_tag="PLU99_09135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17261.1"
                     /translation="MRFAWLDAAWRRPAVVGALILALLGPAAALRIYDLTGKDIWVDE
                     ANGILMARSGLRGLLEHLKLDSSPPLYYLLLGAWMRLFGDSEAAVRSLSVVAGVVTAG
                     AVLAIGWRLFGKTTGLLGAVLVAVAPTHVFYSQQARMYTLLGLLGLLSSYWLWCAIEY
                     GHRRHLFAYALATLGALYTHNFAMHLLPAHALILLWSGAWKRQWKRWAICGLAIATAY
                     LPWLPVFLGQLNNPRQYAWYARFWDKWGIWGAVRGTLFSFSPGEPRPFYQLAETPAIS
                     YSPVLLCGLTLLTGLLLTFAGISANNARIRRAAWLTGATALPLLAALCTSLLSTPNYV
                     GGRVDQMVYPSFALLLAVALSGIRFGFLQRVWTAARYVLAAVLVYNSCAGLAHLYGNQ
                     LSDGDRALARAIASKARPGDAVVFTGLTRAPVEYYLTRWNTPATLFSYPREAAAHLGN
                     LDATALANQPALLQAEVNALQEELRACPPHAQVFVVFTNEPMNDVLGSYLSLPEQRRR
                     WKVLGKFRGAGVRGEALLLLLRPPW"
     gene            complement(4207..5745)
                     /locus_tag="PLU99_09140"
     CDS             complement(4207..5745)
                     /locus_tag="PLU99_09140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17262.1"
                     /translation="MRKSACFALLSAALLGGGLIRVWHVRDRGFWRDEAWVADTVVHK
                     EFAELLTQAEVPLPPLFAVVTKALGSAVGPPELGLRLLPLLCGLAVPALAYLAARSAL
                     APRTLAATAALLCAFSLSLVIWSRELKQYEVEAFASTLAAWLTFGLARWAGKPRTIAV
                     IGLALVCITAPWVGYSCVFALTALLAGLAFMPGRDRRARVICALCLAVAMAFSAAATF
                     QVAAGAQARHPALAQFWQNWYIDLSSPRSLMRAVCLAAFGTTHMLVPVIWFASFDNLV
                     FLLTAAPIWALAAVGLCRWPRRYRVPVACYVIGPWVLMLVAAAAHRYPFTMQRLLVFT
                     APALLMAVAAGLVTAGRACAGVLTGRRGAGMVSAAVIAFVPALYVWQVAWQGRYWSHD
                     DYPRLLSVLKEQRRFNEPVWVTAKAAPPVRYYAGKDLQLALLYPTVAGSLPDSDFDQR
                     QAMNRIFSAPGRYWIITTANPEDDTTELFHWLRARGFKPQLIGEFGSSYWWEAPQLYR
                     LTRN"
     gene            5893..6159
                     /locus_tag="PLU99_09145"
     CDS             5893..6159
                     /locus_tag="PLU99_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOO17263.1"
                     /translation="MNAKLRRELIKRGHGLKAELSVSAGEISDAVVEHVRTYMAGREL
                     GKVRIRADSAAECDEAAAELAERVPCEVVSRVGRVVLLYRTVEG"
     gene            6202..7224
                     /locus_tag="PLU99_09150"
     CDS             6202..7224
                     /locus_tag="PLU99_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HOO17264.1"
                     /translation="MADHVAAAVYGGGWIFVYCAFAVLIGRTRVGAGAAVRWLAFNLR
                     SFAMAVNVACIGGRAAYDLVRKGVFSAERLGPRATPYGESQPLYRCRSKGGDFLLLCR
                     HGEAGYEVTPNLINYRANIYALRDLDVVSVVSWSETRAISHNYKVGQFVVVDDLIDET
                     ISRPTTFFANKELGHIRHWPVFCPNLRKAFVTALEELKLDYSDRGVYVCIEGPRQETP
                     AEAHKYEVFGGELIGQTLAPEVFLAKELQMAYASLCFVASYAETGTPYPAFEAGRVLS
                     EDVLARRADQAVQNMPRILERVFEVLGRKPASIEGRELLRDSDRNGNWRKWFESPRTP
                     TAAENT"
     gene            7221..8429
                     /locus_tag="PLU99_09155"
     CDS             7221..8429
                     /locus_tag="PLU99_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO17265.1"
                     /translation="MIIKAAWVVPVAAPPIKDGFVEISGGRIVRVGRVGQPVCGGADR
                     DFGQAVITPGLVNPHTHLELTAYAGMLEPAPFWKWIESLVWLRRRSGRRERESRGAAE
                     GGWQSLRAGVTCVGDISRENVSWAALKRVPIRKVCFVELLSLADEPPRNPAELRAAVA
                     EVEEDELLTAGVTPHAPYTVSSEHIRAALSLADEIGRPWCTHWAETREEVAFLRGDER
                     ALPLFLGSLLKQCGVRSPACPPGEYLQRCADGLRPGLLAHSNYMDGADAHQIAAGGHS
                     VVYCPRAHAFFGHEAHPFSRLRAAGVRVAIGTDSPASNEDLSILRELQFMRRNCTDLP
                     RADELLRMVTLTAAEALGLERQIGSLEPGKAADLAVFPCAAETVDPIDELIDRALLPV
                     AVWVAGRRVV"
     gene            8593..9321
                     /gene="pyrH"
                     /locus_tag="PLU99_09160"
     CDS             8593..9321
                     /gene="pyrH"
                     /locus_tag="PLU99_09160"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOO17266.1"
                     /translation="MSETEPRYKRVLLKVSGEGFCPPGGFGIHRAELDHVAHEIAEVA
                     RLGVQIGVVVGGGNFLRGARFARELGMDPSTADYMGMLGTVLNALALHEALERAGLAT
                     RVQSALTIARVCEPFIRLRAIRHLEKGRVLILAGGTGNPHVTTDSAAALRAVEIKAQA
                     LLKATKVDGVYDSDPKLNPAARRYEHLTYDQVIDGRLKVMDLSAVDLCQQHQIPIVVF
                     NLFQQGTMRRVLLGEQVGTLVNDR"
     gene            complement(9245..>10201)
                     /locus_tag="PLU99_09165"
     CDS             complement(9245..>10201)
                     /locus_tag="PLU99_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17267.1"
                     /translation="VPDENHPSRQGLPFDWYLELLRFIKREYPSIHIHAFSPPEIWAF
                     HRNFGLPLRDVLLRLQEAGLDTIPGGGGEILVDRVRQKIGRGKAMTDEWLAVMREAHR
                     LGMKTSCTMMFGHIETVPERIEHLRRLRDLQDETGGFTAFIHWPFQPDGTPLGRWKQP
                     PQERAVPSLGAAPGLAPELASAPRPLPDGEHLFLADAHEYLVMLALARLYLDNIPNIQ
                     SSWVTMGPKIGQLALFFGANDMGSVMMEENVVSAAGTTFRLNEETIRRLIADAGWQPQ
                     QRDQYYRPVTRPDRPSDANDHSPASPPARPAERASSFLAGKD"
BASE COUNT         1727 a         2997 c         3668 g         1809 t
ORIGIN      
        1 gggcgggttc catggcggct tttgctttgc cgctgtgggc tttgcccgct cgcttgcgct
       61 cggggctcgg tttcctgccg cctgcggcgc tcgcgggtcc ggctgcctcg gctgcgccag
      121 ccttggctcc gccggcggtc gccggggcga tgggccggcc gtcttcgtcc agttgtacgt
      181 cggcctcggc cgtgcgcatc ttggcgatgg tgcttaactg cttgaccagc cggtcctcgc
      241 tcaggccggt gaggttggcc agggcttcgg ccacgtcggg aatgtagcgg ccgaagatgc
      301 tgcggcggtc ggcttccatg cgctggcggc ggcggcggcg caggtaggcg gccagcttgc
      361 ggccgcactc ctgcaaggcc agcttcatct ccttgcggat ttcgtcgtaa tcggcgatgg
      421 cttccttgct ctcgctggtg aaggggaccc agacgctggc catgtgcacc atgacgacca
      481 gcgggccgac cggcaggctg ttgccgggct gcgagaggcc gtagttgcgc cagtccacgt
      541 cgagcacgga cttgaaggcg gcgcaggcag actgctggta cagcagcggg acgcggttgg
      601 cgaagcggat gacgcgcgcc ggctggtcgg cgggcaggct gccgccgtag gccagggcga
      661 tttcgatctg gaaggggttg ccgcgataga cggccgggtc gcgcgtggtg gcggtgtaga
      721 actcggcctt gacctctttg agcaggccgg ccaggaggtt gcgcacgccg atgggggcca
      781 ggcagttggt gggcggggcc atgatcttga cggcctgcat ggcctggtag aggcgttcga
      841 cgtcgtgcgt gccgatctgg gccgggcggg ccttggggct ctggccggcg tgctcgcaga
      901 tggcggccgc gacgcgcggc gtgacgcgtg agaactcgcg ctgcaggaag ccggagaggc
      961 tcggggtgcg ttcgcccttg cggtcggggc gggcgtcctt gagcattttc atcagcacgc
     1021 ccagctcgat gccgtgcggg tggggcttga tctcgcaggt ttcggccggc agctcggtgg
     1081 cgccgcgcgg gaagtcgcgg tgctcgccgg tgggggcacg gtaatgaatg agggcgtgcg
     1141 ggttggcgat ggcggtctgc tcgacgtact cgtcgaccga ctggcggcct ttggcgtagg
     1201 tggcgaccag ctcgatgacg acctccgtgc cgcgctccca cgcgatgtcc gggatttcct
     1261 cgtccttgat gacgtcggcc cggttgcgcg ccgtgtccat ctggatttcg aggtagtggg
     1321 cgggcttttt ggggctggtg cgcgagatta tgtgcacgct cttgccggtg gtgagcagcc
     1381 cgtacatgcc ggccgcggag atgccgatgc cctgctggcc gcgggacatc ttgaggcggt
     1441 ggaatttcga gccgtagagc agcttgccga agatgttggc gatctgcgac ttgatgatgc
     1501 cggggccgtt gtcgcggacg gtgacgcgga agcggtcctc ggcgagctgg tcgatgtgga
     1561 tgtggatttc gggcaggatg tgggcttctt cgcaggcgtc gaggctgttg tcgacggctt
     1621 cctttacggt ggtgagcagg gccttgcgct tgttgtcgaa gccgagcagg tggcggttct
     1681 tggcgaagaa ttcggaaacg ctgatgtcgc gctgtttgtt ggcgagcgtt tcggccgtca
     1741 cggtgctgga gcgcggggcg cggacggcgg gcggtgtatt gtcggcgtca gcgggcgcgg
     1801 cgggcggctc gggggcgggc gcaggggcgg cgtttgcggg ggccgactcg ggcgcagcgg
     1861 cggattttgc aagcgcgggc gcagcggcgg atttcgcggg ctggatgggc tcggtcgatt
     1921 cctttgattc tctcctggag gcctttgact ccttcttggc agccttcgat tctactttgc
     1981 cggccttcga tcgttcgggt tgaaagcggg tctggcggag cttggtggcc ggccggcggt
     2041 tggggcgtcc gttttcgccg tcggtagcgg tcgtttccgc aaggtagacc gcctggctga
     2101 cggtgccgac cggaatgggg cgcagtttgg gtggagtgac ggcttcgaag agcgatgggg
     2161 tatcctcagc cgacgacctg cggcggcggg ttttttccat ccgtggactc caggaattat
     2221 gtcgaacgcc ttcgcgaagg ctgtttctgg agcttatcgt agcacgctcg cccggcctgt
     2281 acaggcaagc gggaaccgag ctcggcccgg cagttgaccg tggttcgcgg tccggcgctg
     2341 ggctgtcgct gccgggtgcg cccgagggca tcgccacgac ggaggctagc acgcgccgac
     2401 cgttccgcag cggcgcgcct accagggagg gcgcagcaac agcagcaacg cttcgccccg
     2461 cacgccggct ccgcgaaatt tgcccaacac cttccagcgc cggcgttgtt ccggcaagct
     2521 caggtagctt cccagaacgt cgttcatcgg ctcgtttgtg aacaccacga acacctgcgc
     2581 gtgtggtggg caggcccgca gttcctcttg cagcgcgttt acctccgctt gcagcaaggc
     2641 cggctgatta gccaacgcgg tcgcgtccag gttgcccagg tgggcggccg cctcgcgggg
     2701 gtaggagaac aatgtggccg gcgtgttcca gcgcgtcagg tagtattcca ccggtgcgcg
     2761 cgtcagtccg gtgaagacca cagcgtcgcc cgggcgggcc tttgatgcga tcgcgcgcgc
     2821 cagtgcccgg tcgccgtcgg aaagttgatt gccatacagg tgcgccaggc cggcgcacga
     2881 attgtacacg aggactgccg ccaagacata acgggccgca gtccagaccc gttgcaggaa
     2941 accgaagcgg atgcccgata gtgccaccgc gagcagcagc gcgaaactcg gatagaccat
     3001 ctggtctacg cgaccgccga cgtagtttgg agtgctcagg agggacgtgc atagcgccgc
     3061 cagcagcgga agcgcagtcg cgccggtcag ccacgcggcg cgccgtatcc gcgcattgtt
     3121 cgccgaaatc ccggcaaagg taagcaacag accggttagc agcgttagtc cacagagcag
     3181 caccggcgaa tacgaaatgg ccggcgtttc cgcaagttgg taaaacggac gcggctcgcc
     3241 gggcgaaaag gagaacaggg taccgcgcac tgctccccaa atgccccact tgtcccagaa
     3301 gcgcgcgtac caggcgtact gccgcgggtt gttcaactga ccgagaaaga ccggcagcca
     3361 gggcaggtag gcggtcgcta tcgccagccc gcagatcgcc cagcgtttcc actggcgctt
     3421 ccatgctcct gaccatagga ggatcaaggc gtgcgccggc agcaggtgca tcgcgaagtt
     3481 gtgcgtgtag agcgcgccta gcgtggccag tgcgtaagcg aaaagatgcc gcctgtgtcc
     3541 gtactcgatc gcgcaccata gccaatatga agacagcagg cccagcagtc ccagcagtgt
     3601 gtacatccgc gcctgctgcg aatagaacac atgggtcggc gcaaccgcca ccaggaccgc
     3661 tcctagcagg cccgtggtct taccgaacag ccgccaaccg atcgccagca ccgcgccggc
     3721 ggtcaccacg ccggcaacca cggacaacga ccgcacggcg gcctcgctgt ctccaaacag
     3781 ccgcatccag gcgcccagca gcaggtagta caagggcggg ctggaatcca gtttgaggtg
     3841 ttcgagcagg ccgcggaggc cggaccgcgc catcaggatt ccgtttgctt cgtcgaccca
     3901 gatgtccttg cccgtcaggt cgtaaatgcg cagggctgca gccggaccca gcagagccaa
     3961 gatcagcgcg ccaacaaccg cggggcgccg ccaggcggca tccagccacg cgaatcgcaa
     4021 ggcgccaggc ctttccgcaa cggtgttggc tggttgtgtc atcgcggacc gtttcgtggt
     4081 gcgcatgggt tgagatgcta cacggacgcc gcgaaagaaa atagggcttg gaacggggcg
     4141 cgcgccgcgg cgcgcgttgg ccattggcgg aacgcgggcg atagttcgat cacgcaatac
     4201 gacgtgctag ttccgggtca atcggtacag ttggggcgcc tcccaccagt acgaactgcc
     4261 gaactccccg atcaactgcg gcttgaagcc gcgcgctcgc aaccagtgga aaagttcagt
     4321 cgtatcgtct tccgggtttg cagtagtgat gatccaatac cggcccggag cactgaagat
     4381 gcggttcatc gcctggcgtt gatcaaagtc cgaatccggc aggctgcccg caactgtggg
     4441 atacagcagg gcgagctgaa ggtccttgcc tgcgtagtaa cgcactggcg gcgcggcctt
     4501 ggccgtcacc cagaccggtt cgttaaagcg acgttgttct ttcaacacgc tcagtaagcg
     4561 gggatagtca tcgtgcgacc agtatcggcc ttgccacgca acctgccaga cgtacagagc
     4621 cggcacaaag gcgatgactg ccgcgcttac catcccggcc ccgcgccgac ccgtcagtac
     4681 gccggcacat gcgcggccgg cagtcaccaa tccggccgcg acggccatga gcagcgccgg
     4741 cgcagtgaac accagtaggc gctgcattgt gaatggatag cggtgagcag cggccgccac
     4801 aagcatcagc acccaaggcc cgataacata gcaggccacg ggcacgcggt agcggcgcgg
     4861 ccagcggcac agtcccacgg cagcgagcgc ccagatggga gcagcggtaa gcagaaagac
     4921 cagattgtcg aacgaggcga accatattac cggcacgagc atgtgagtcg ttccgaaggc
     4981 cgccaggcag acggcgcgca tcagagacct gggtgagctc aggtcgatgt accagttctg
     5041 ccagaattgg gcaagtgccg ggtggcgcgc ctgagcaccg gcagccacct ggaaggttgc
     5101 cgcggcagag aaagccatcg ccacagctag acacagcgcg caaatgacgc gcgctcgccg
     5161 gtctcgcccc ggcatgaacg caagtcctgc caaaagcgcc gtcagtgcga agacgcacga
     5221 gtagccgacc cagggggccg ttatgcaaac caatgccagg ccgattacgg cgatcgttcg
     5281 cggcttgccc gcccagcggg cgagaccgaa ggtcagccat gctgcgagtg tcgacgcaaa
     5341 ggcctcgacc tcgtattgct tgagttcgcg actccatatc accaggctca ggctgaaagc
     5401 acatagaagg gctgccgtcg ccgccaaggt gcgcggggcc agcgctgagc gagcggcgag
     5461 gtaggccagc gccggcacgg ccagaccgca caacaatggc agaagcctca accccagctc
     5521 aggaggcccg accgccgacc ccagggcctt cgttaccacc gcaaagagcg gcggcaacgg
     5581 aacctccgcc tgcgtgagca gttcggcaaa ttccttgtgg accaccgtgt cggcgaccca
     5641 ggcctcatcc cgccagaaac cgcggtcgcg gacgtgccag acgcggatga gcccgccgcc
     5701 gagcagcgcg gcggaaagca acgcgaaaca agcgcttttc ctcattcgtc tcatcagcct
     5761 gccctcaact cggggctcgc ggtgcctggc aagtagcgga ttaaattccg actcattatt
     5821 ctgatacggc cccctcagca ccgcaagcct ttttgtcggg cggcggttgc cgggtgtcgc
     5881 gcggagatga ctttgaatgc aaaacttcgg cgggaactga tcaaacgcgg gcacggcctg
     5941 aaggcggagc tgagcgtatc cgcgggcgaa atttcggacg ctgtggtcga acatgtacgg
     6001 acgtacatgg cgggtcgcga actgggcaaa gtgcgaattc gggcagatag cgcggcggag
     6061 tgcgacgagg ccgcggcgga gctggcggaa cgcgtgccgt gcgaggtcgt gtcgcgcgtg
     6121 gggcgagtgg ttctgctgta tcggacggtt gagggctgac ggggcgacct ggccgcagac
     6181 ttgcgtctgg ggtactgatc agtggctgat catgttgcgg ctgcggttta cggcgggggg
     6241 tggattttcg tatactgcgc ttttgctgtt ctgatcgggc gcacgcgtgt gggcgcgggc
     6301 gcggcggtgc gctggctggc gttcaacctt aggagctttg cgatggcggt caacgtggcg
     6361 tgcattggcg ggcgtgccgc atacgacctc gtccggaaag gggttttcag tgcggagcgg
     6421 ctcgggccgc gggccacgcc atacggcgag tcgcagcccc tgtatcgttg ccgcagcaag
     6481 gggggcgatt ttctgctgct gtgccggcat ggcgaggcgg ggtacgaggt gacgccgaac
     6541 ctgatcaact atcgcgcgaa catttacgcc ttgcgcgacc tggacgttgt gtccgtggtc
     6601 tcgtggagcg agacgcgggc gatcagtcac aactacaagg tggggcagtt cgtggttgtc
     6661 gacgacctga tcgacgagac tatttcgcgg ccgacgacat ttttcgccaa caaggagctg
     6721 gggcacatcc ggcactggcc ggtgttctgc ccgaacctgc gcaaagcgtt cgtcaccgcg
     6781 ctggaagagt tgaaactgga ctattcggat cgcggcgttt acgtatgcat cgaggggccg
     6841 cggcaggaaa cgcccgccga agcgcacaaa tatgaggtct ttggcggtga attgatcggc
     6901 cagacgctgg cgcccgaggt cttcctggcc aaagagctgc agatggccta tgccagcctg
     6961 tgcttcgtgg ccagctacgc cgagaccggt acgccttatc cggcgttcga ggccgggcgc
     7021 gtgttgagcg aggatgtact ggcgcggcgg gcggatcagg cggtgcagaa catgccgcgg
     7081 attctggagc gggtgtttga ggtactgggg cggaagccgg ccagcatcga gggacgcgag
     7141 ctgctgcggg atagcgatcg caacggtaac tggcgaaagt ggtttgagag cccaaggaca
     7201 ccgacggcgg cggaaaacac gtgatcatca aggccgcctg ggtggttccg gtcgcggcac
     7261 cacctatcaa ggacggcttc gtggagatca gcggggggcg tatcgtgcgg gtcggccgcg
     7321 ttgggcagcc ggtctgcggc ggagccgatc gcgatttcgg gcaggcggtc ataacgccgg
     7381 ggctggtaaa tcctcatacg caccttgagc tgacggcata cgcggggatg ctcgagccgg
     7441 cgccgttctg gaagtggatt gagagcctgg tgtggctgcg ccgcaggagc gggcggcgag
     7501 agcgggagag ccggggggcc gccgagggcg ggtggcagtc gttgcgggcc ggggtgacct
     7561 gcgtgggcga catttcgcgt gagaacgttt cctgggcggc gctgaagagg gtgccgatcc
     7621 gcaaggtttg tttcgtggaa ttgctttcgc tggccgatga gccgccgcgg aatccggccg
     7681 agctgcgggc ggccgtagcg gaggtcgaag aggacgagct gctcacggcc ggggtgacgc
     7741 cgcatgcgcc atacacggtg tcgagcgagc atattcgggc ggcgctttcg ctggcagacg
     7801 aaatcgggcg gccatggtgt acgcactggg cggaaacgag ggaagaggtg gcgttcttgc
     7861 ggggcgacga gcgggcgctg ccgttgttct taggcagctt gctgaagcag tgcggcgtgc
     7921 gttcgccggc ctgcccgccg ggcgagtatt tgcagcggtg tgcggacggg ctgcgacccg
     7981 gattgctggc gcacagcaac tacatggacg gtgcggatgc gcaccaaata gccgcgggag
     8041 ggcacagcgt ggtttattgc ccgcgggcgc acgcgttttt cgggcatgag gcgcatccgt
     8101 tttcacgcct gagggcggcg ggcgtgaggg tggcgatcgg caccgacagt ccggccagca
     8161 acgaagattt gtcgatcctg cgggagctcc agttcatgcg tcgtaactgc accgatctgc
     8221 cgcgggcgga cgagctgctg cggatggtaa cgcttactgc ggccgaggcg ctgggattgg
     8281 agcggcagat aggttcgctc gagccgggca aggccgctga tctggcggtg tttccgtgcg
     8341 cggcggagac ggttgacccg atcgacgagc tgattgaccg cgcgctgctg ccggtcgcgg
     8401 tttgggtggc gggcaggcgg gtggtttagt tagttccaga cgcgggggaa atgtgcgaat
     8461 aaccaacccg gcgcgtcgaa agctttgccc gctggccatt ccggccattc ggcgacgctt
     8521 gggtgtggcg cgggaatgcg gtagcatgcc ccggagcgct gcggcgcgta cgtatggcgt
     8581 gggtgtgact aaatgagtga aactgagcct cggtacaaac gtgtgctgct gaaggtcagc
     8641 ggtgaaggtt tttgcccgcc gggcgggttt ggcattcatc gagccgaact ggatcacgtg
     8701 gcccatgaga tcgcggaagt ggcgcgtctg ggggtgcaga tcggggtcgt ggtcggcggc
     8761 ggaaacttcc tgcgcggggc gcgcttcgcc cgcgaactcg gcatggaccc gtcgaccgcc
     8821 gactacatgg gcatgctggg gacggtgctg aacgcgctgg ccctgcacga ggctctggag
     8881 cgggccggcc tggcgacgcg cgtgcagagc gcgctgacga ttgcccgcgt ctgcgaaccc
     8941 ttcatccgtc tgcgcgccat ccggcacctc gaaaagggcc gcgtcctgat tcttgcgggc
     9001 gggaccggaa atccacacgt aaccaccgac tcggccgccg cgctgcgggc ggtagagatt
     9061 aaggcccagg ctctgctcaa ggccaccaag gtagacgggg tctacgacag cgaccccaag
     9121 cttaacccgg cggcgcggcg ctacgagcac ctgacctacg accaggtgat tgacgggcgg
     9181 ctgaaggtga tggacctcag cgcggttgat ctatgccagc aacaccagat tccgatcgtg
     9241 gtgttcaatc ttttccagca aggaacgatg aggcgcgttc tgctgggcga gcaggtgggg
     9301 acgctggtga atgatcgtta gcgtccgagg ggcgatccgg gcgtgtgacc gggcggtagt
     9361 attggtcgcg ctgttgaggt tgccagccgg catccgcgat tagccggcgg atcgtctctt
     9421 cgttcaggcg gaacgtagtt ccggcggcgg agactacgtt ctcctccatc attactgagc
     9481 ccatgtcgtt ggcgccaaag aaaagagcca gttggccgat cttcgggccc atggttaccc
     9541 agctcgattg aatgttcgga atgttgtcca gatacaagcg cgccagggcg agcatgacca
     9601 ggtactcatg ggcgtcggcc aggaagagat gctcgccgtc gggaaggggc cgcggagcgg
     9661 atgcgagctc gggcgccaag ccgggcgctg cgcccaagct tggcacggcg cgttcctgcg
     9721 gtggctgttt ccacctcccc agcggcgtgc catccggttg gaacggccaa tgaatgaatg
     9781 cagtgaagcc gccggtttcg tcctggagat cgcgcagccg gcgcaggtgt tcaattcgct
     9841 cgggcacagt ctcaatatgg ccgaacatca tggtgcagct tgtcttcatg cccaggcgat
     9901 gggcttcgcg catgacggcc agccattcgt cggtcatggc tttgccgcgc ccgatcttct
     9961 gccgcacgcg gtcgaccagg atttcgccgc cgccgccggg gatcgtatcc agtccggctt
    10021 cctggagccg tagcaacacg tcgcgcaggg gcaggccgaa gttgcggtga aaagcccaaa
    10081 tttcgggcgg gctgaaggcg tggatgtgaa tgctgggata ttcgcgcttg atgaagcgca
    10141 gcaactcaag ataccaatcg aagggcagcc cttggcggct ggggtgattt tcgtccggca
    10201 c
//
LOCUS       DAOSYS010000128        10198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5598_length_10198_cov_4.526669, whole genome shotgun sequence.
ACCESSION   DAOSYS010000128 DAOSYS010000000
VERSION     DAOSYS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10198
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_10198_cov_4.526669"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLU99_09170"
     CDS             complement(<1..241)
                     /locus_tag="PLU99_09170"
                     /inference="COORDINATES: protein motif:HMM:NF036812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOO17268.1"
                     /translation="MDTAVLRIVDVNLNRAREALRVIEDYARFALDDVDAAAAVKHCR
                     HQLRDLAAGFGPRELLAARDIANDVGRDAKTPAELQ"
     gene            602..1534
                     /locus_tag="PLU99_09175"
     CDS             602..1534
                     /locus_tag="PLU99_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOO17269.1"
                     /translation="MRTSLAGLLVNAALAIVKLTAGLVGHSYALVADAVESTSDVLAS
                     LIVWRGLRIAAQPADEEHPYGHGRAEPLAAMVVALMLVVAGIGIAIEAVQGILTRHAV
                     PAPFTLYVLLGVVVIKEAMFRFVGRVGRDVRSRAVATDAWHHRADAITSALAAVGISI
                     ALIGGPKYAQADAIAALGGAGVILFNAYRLLMPPLHELMDVVPTETVARARGVAELVP
                     DVAGVEKVLARKVGLRYLVDMHVEVDREMSVHHAHEVAHAVKDAIRLAMPEVENVLVH
                     IEPYNAEHTRQPPEPSASSPPAGPSPASSSPTGA"
     assembly_gap    1678..1777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1778..2221)
                     /locus_tag="PLU99_09180"
     CDS             complement(<1778..2221)
                     /locus_tag="PLU99_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOO17270.1"
                     /translation="MDEPNLHEVLEFAVEAAQLAGAVTLGYFNANTPFELKADRSPVT
                     AADRGAEQLLRARIQAAFPEHGILGEEFGEQPGGAAGRWILDPIDGTFSFLSGVPLYS
                     VLVGFEWRGEMLAGVMHFPALHETLYAARGLGCWCNGRRARVSDVS"
     gene            complement(2152..>2992)
                     /locus_tag="PLU99_09185"
     CDS             complement(2152..>2992)
                     /locus_tag="PLU99_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO17271.1"
                     /translation="EGANQPSGCGADCCREPAALQSPDETAAGEAASPAFSDRPLRTA
                     LRHTFVNLFDDIVGWVLLGILAAAAIQTWIPPSTLTSVMGGQLQSMLLMLLIGVPLYV
                     CADASTPIAAALIAGGVSPGAALVFLLAGPATNIGTLGVLARQFGRQAVAIYLASIVV
                     VALAAGALVDLAAGGSARALTANLGEQSLLPEWLKTAGAVAFLAWGVFSVVRLRYLPR
                     GAAWFRAVLKKLANRRSAFETETGAAAQIAVAVDGGLEKWTSQTCTKCSSSPLRQPSS
                     PER"
     assembly_gap    2993..3092
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3581..5785)
                     /locus_tag="PLU99_09190"
     CDS             complement(3581..5785)
                     /locus_tag="PLU99_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOO17272.1"
                     /translation="MPQKLRELARAANLNDFESRCLELLDSGNLPLAEVAECLSLIEQ
                     TGKAERVAPLAQMIFENIDVAAQPREALKVASAALLATPKSDQLRQLTLDLYRRVYGQ
                     TPGFDAILESAGLTTGAAPRAALRVLDFCLALEVGDTLISRMDDRVVEVAQVDRLRGL
                     FTLRRADRVTTIPARELVREYERIDPKDFRVLRQLHPERIGELIESDPVALVIGLINA
                     YGQHIDADQLRYELVPRYIEPKSWSKWWTRAKAELKRSPNIIMEGRSPIVLRYSPEGV
                     SLEQEVWEQFTAVSDPEKWLDIAESYLREQKSRKAAPDKDLLHRFREHLRKYAADIRT
                     RRPSEAFACALVAAEIARAEGEPANGLAGEMLAASPQPARWIAELKHDVLWERAFAAL
                     PQARPADWPEVVVALLPLSPAARFDRLAEALRTAGRLAELQRLIDDALVDPVNYPEPI
                     YWLWKGPKNPEGLTLPEDTALFATILDTVVALDTTLTPAPQVGRNFRNRVKSAFALRD
                     YAKVAACLRQISTAAAVPLKRQIERLEGLGDNARIAMTEALRSAHPELWITHEVALAP
                     WEDPNTLWATPAGIEKKTAERDELVNVKMRENAKRIGEAGALGDLSENSEYKFALEER
                     DFLRARLAQINHDLSMARPLSPSEVATHHVSIGTRVTLRESDGSGTASRVLTIIGPFE
                     ADIDAGIFSYKAPVPQKLMGLRVGERTKLPLEGGEREYEVVSIENALADGPG"
     gene            complement(5842..6195)
                     /locus_tag="PLU99_09195"
     CDS             complement(5842..6195)
                     /locus_tag="PLU99_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17273.1"
                     /translation="MVIDAYDKNADVVNFMNVQGALVSPTHEASPLRLARTGPSRRGN
                     YVLNFKYQMANGTPARCGTLEIAVPAAFDHASLARLETPSSTAYGLARPTGRKPAAGE
                     AKKRVRQDLEKDQQG"
     gene            6518..7996
                     /locus_tag="PLU99_09200"
     CDS             6518..7996
                     /locus_tag="PLU99_09200"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOO17274.1"
                     /translation="MLNQPGSDHPLPSLFWIWPFAALLLAIAILPLVRRTHHWWEHNA
                     NKLLVSLVLAAVTLVYYGLRGYGVESHDKEAAPAAASAPAAADAGQPQLEAEPEAHAP
                     TYTARGWPTVQAVLRHAVLGEYVPFIVLLFSLYVIAGGIVVRGDIQATPAANTAILGI
                     GGALASIIGTTGAAMVLIRLLLKTNSERKRKVHTIVFFIFIVANAGGLLLPTGDPPLF
                     LGYLYGVNFLWTLRLWPQWLFVLGVLLVVYFLWDTREYGRESPADLARDVSIVRPIRV
                     AGLLNMVWLVGVVVAVGTLDPSKPVPGTNWYPFPYLREMVQISLVVISVLTTHAKLRV
                     ENQFTYAAIGEVACLFIGIFITMQVPIEILQARGPQLGLSAPAHFFWATGLLSSFLDN
                     APTYAVYFATAGALPPSGMPLVQGVQTATGAIAAPLLAAISCGAVFMGANTYIGNGPN
                     FMVRAIAEQSGVRMPSFFGFMIYSGLVLIPLFVVMTFIFFWS"
     gene            8223..8891
                     /gene="nth"
                     /locus_tag="PLU99_09205"
     CDS             8223..8891
                     /gene="nth"
                     /locus_tag="PLU99_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOO17275.1"
                     /translation="MRTGESQTARSARALRILKILRATYPDATTALRHRSAYELLVAT
                     ILSAQCTDERVNQVTPDLFRRYPDAAALAEADPAELEAGIKSTGFFRNKTKSLLGAAK
                     MIRDRFGNRVPDTMAELLELPGVARKTANCVLGTWYHKNEGVVVDTHVGRVALRLGLL
                     TTARNDKDAVKIEQDLMALLPRESWTFVSHALILHGRRICTARKPKCGECPLNADCPS
                     AFKV"
     gene            complement(8961..9176)
                     /locus_tag="PLU99_09210"
     CDS             complement(8961..9176)
                     /locus_tag="PLU99_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17276.1"
                     /translation="MIKSKKETPPPGNPLPMKRNAWEVAFYAASILAVLAFVLASVVG
                     RSVTLSLVGAVFMATAAIIADKRQSRP"
BASE COUNT         1664 a         3088 c         3448 g         1798 t
ORIGIN      
        1 gctgcaactc cgcgggtgtt tttgcatcgc gtccgacatc gttggcgatg tcgcgggccg
       61 ccagcagttc gcgcggtccg aaaccggccg cgaggtcccg aagctggtgg cggcagtgtt
      121 tgacggccgc ggccgcgtca acgtcgtcca gggcgaagcg cgcatagtcc tcgatgacgc
      181 gcagcgcttc gcgtgcgcga ttgagattga cgtccacgat gcgcaggaca gcggtatcca
      241 agctttcgcc cttatgagcc gttaagcacg ggcccggctt cagcgtactc cacgagccgg
      301 cggacgcaag cggggcggca cgccccggct ctgctctgat gtttggggct cgatcaaaac
      361 gatttcgggt cctacactct ttgtaatgag tggagattcg cagacactgc acccgcccgg
      421 accaggcggt cacatcccga gtggccgcgg agcgcgcgag cgcgtcctgc cgggctgctt
      481 tccggacgat cgtgcgccga agggcggcgt tccggatggc cgcattcaga atggtcgcgc
      541 tccgatcggt tgcgtcccag atgggcgcgt tgccggcgcc ggcgacggtt atgagcgcgg
      601 catgcgcact tcgttggcgg ggctgctcgt caacgccgcc ctggcgatcg ttaagttgac
      661 cgcgggcttg gtcggccatt cgtacgcgct cgtcgcggac gccgtcgagt ccacgtccga
      721 tgtactcgcg tcattgatcg tctggcgtgg tttgcgtatc gcggcccagc cggcggacga
      781 ggagcatccc tacgggcacg ggcgtgccga gcccttggcc gccatggtcg tggcgctcat
      841 gctcgtcgta gccggcatcg gcatcgccat cgaggcggtg cagggcatcc tcacgcgaca
      901 cgcggtgccg gcccccttca cgctttacgt gctgctgggc gtggtcgtca tcaaggaggc
      961 catgtttcgt ttcgtcggcc gcgtcggacg cgacgtgcgc agccgcgccg tggccaccga
     1021 cgcctggcac catcgcgccg atgcgatcac gtcggcgctg gcggcggtgg gtatcagcat
     1081 tgctctaatc ggcggcccca agtatgccca ggcggacgcc atcgcggccc tgggcggcgc
     1141 cggggtgatt ctgttcaatg cgtaccggct gctgatgccg ccgctgcacg agctgatgga
     1201 cgtcgtgccg acggaaacgg tggcgcgggc ccggggtgtg gccgagttgg tcccggacgt
     1261 ggcgggcgtc gagaaagttc tggcacgcaa ggtcggattg cgctacctgg tcgacatgca
     1321 cgtcgaggtc gatcgggaaa tgtcggtaca tcacgcgcac gaagtcgcgc acgcggtaaa
     1381 ggatgccatc cggctggcaa tgccggaggt cgaaaacgtc ctggtgcata tcgaacctta
     1441 caacgccgag cacacgcggc agccgccgga accatcggcc agctcaccac cggcagggcc
     1501 atcgccggca agctcatcgc cgacgggcgc ttaggcgtcg taagcggtgc gcgcgccctg
     1561 acgaacgctg cgttcgtcca acagcctcag caccgggttc aggagccggc cattcgtccc
     1621 gaccaccgcc ggcgcggtat gcgtctgccg gccggtccaa tcggtgaacg taccgccnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct cacatcagac acccgcgccc
     1801 gccggccgtt gcaccagcag cccagcccgc gcgccgcata gagcgtttcg tgcagggcgg
     1861 ggaagtgcat gacgccggcc agcatctcgc cgcgccactc gaagcccacc agcacgctgt
     1921 agagcgggac gccgctgagg aatgagaagg tgccgtcaat cgggtccagt atccatcgcc
     1981 ccgcagcgcc gccgggctgc tctccgaact cctcgcccaa tatgccgtgc tcgggaaagg
     2041 ccgcctggat gcgggcgcgt aagagctgct cggcgccgcg gtcggccgcc gttacgggcg
     2101 agcggtcggc cttgagttcg aacggcgtgt tcgcgttgaa gtaaccgagc gtcaccgctc
     2161 cggcgagctg ggctgcctca acggcgaact cgagcacttc gtgcaggttt ggctcgtcca
     2221 tttttccagc cctccatcaa cagccacggc gatctgcgcg gctgcgccgg tttccgtctc
     2281 gaacgcggac cggcggtttg ccagcttctt cagaacggca cggaaccagg ccgcgccccg
     2341 cggcaaatat cgtaagcgaa ctacgctgaa aacgccccat gccagaaaag cgaccgctcc
     2401 ggccgtcttc agccactccg gcagcagcga ctgctcgccg aggttggcgg tcagcgcgcg
     2461 ggccgatccg cccgcagcca ggtcgaccag tgcgcccgcg gccagcgcaa cgaccacgat
     2521 ggaggccaag taaatggcga ctgcctgccg gccaaactgc cgcgccagca cgccgagcgt
     2581 gccgatgttt gtggccggcc cggccagaag aaagaccagg gcggcgccgg gactcactcc
     2641 gcccgcgatc aaggcggccg caatgggtgt tgaagcgtcg gcgcacacgt aaagcggcac
     2701 gccgatgagc aacatcagca gcattgattg caactggccg cccatgacgc tggtcagggt
     2761 gctcggcgga atccaggttt gtatggcggc cgcggcgaga atgccgagga gcacccagcc
     2821 gacaatgtcg tcgaacagat ttacgaacgt gtggcggagg gcggtgcgta gagggcggtc
     2881 ggagaatgcc ggcgatgcgg cctcgccggc tgcggtttcg tcgggcgatt ggagcgcggc
     2941 cggctcgcgg caacagtcgg cgccgcaacc gctgggctgg tttgcgccct cgnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgccgat cagcgtggcc cagaaaacag
     3121 agcgccgccc gttcccgcgc agcagcttcg ccagccgcag gcgagagaat agcgcatcca
     3181 gcaaggccgc cagcagaaaa ccaagcagca ggaagagcgc ggagtcgcgc agtagttccc
     3241 aagcgttcga aagcaccgtg gactcgtttt ttggggctcg ttctcttggt tcggcccgtg
     3301 ataaggggat tgtaggaagc gcttccgcgc ttgccgtaag gaccgcgcgt aatgttgggt
     3361 gattttaggt gattgtgggt gatttgggtg attgtgggcg ccatgcccaa gctataagcg
     3421 cgggcatggc ctcgttgacg gcgccatatg tgaggcaacc ggcgacggca tgcccgcgcc
     3481 gctacgcggc ttgggcatgg caccccggct tgggcatggc gccctgcatg gcgcgcacct
     3541 ttaccgcacc atgcgggccg tcgtgaatcg ggccccgaaa tcagccgggg ccgtcggcca
     3601 aggcgttttc gatggagacg acttcgtatt cgcgctcgcc gccttccaac ggcagcttgg
     3661 tccgctcgcc tacgcgcagg cccatcagct tctgcggtac cggggctttg tagctgaaga
     3721 tgcccgcgtc gatgtcggcc tcgaacgggc caatgatggt cagcacacgc gatgctgtgc
     3781 ccgagccgtc gctttcgcgc agcgtgaccc gcgtgccgat tgaaacgtgg tgcgtggcga
     3841 cctcggacgg gctcagcggg cgggccatcg agaggtcgtg gtttatttgg gcgagccggg
     3901 cacgcaggaa gtcgcgctcc tccaacgcga acttgtattc ggaattctcg ctcaggtcac
     3961 ccagggcccc ggcctcgccg atgcgcttgg cgttttcgcg catcttgaca ttcaccagtt
     4021 cgtcgcgttc agcggtcttc ttttcgatgc cggcgggcgt ggcccagagc gtgttggggt
     4081 cttcccacgg cgctaacgcg acctcgtgcg tgatccacag ctcgggatgc gcggagcgca
     4141 aggcctcggt catcgcaatg cgggcgttgt cacccagacc ctccagccgc tcaatctgac
     4201 gtttaagcgg aacggcggcg gccgtgctga tctggcgcag gcaggcggcc accttcgcgt
     4261 agtcgcgcag tgcgaaggcg gatttcacgc ggttgcggaa attgcggcca acttgcgggg
     4321 cgggcgtgag ggtcgtgtcg agcgcgacca ccgtgtcgag aatagtcgcg aacagggccg
     4381 tgtcctcggg caaagtcaac ccttccgggt tcttggggcc tttccacagc cagtagatcg
     4441 gctctgggta gttgaccgga tcgaccagcg catcgtcgat caggcgctgc aattcggcca
     4501 accgaccggc cgtccgcaag gcctcggcca agcgatcgaa ccgggcggcg ggggaaaggg
     4561 gcagcagggc caccacgacc tccggccagt cggcgggtcg ggcctgcggc aacgcagcaa
     4621 aggcccgctc ccagagtacg tcgtgcttga gttcggcgat ccaccgggcc ggctgcggcg
     4681 atgccgccag catctcgccc gccagcccgt tggccggctc gccttcggcc cgggcgattt
     4741 cggccgccac gagcgcacaa gcgaaggctt ccgacgggcg gcgggtgcgg atatcggccg
     4801 catacttgcg cagatgttcc cggaaacggt gcaggaggtc cttatcgggg gcggctttgc
     4861 gcgacttctg ctcgcgcagg tagctttcgg cgatgtccag ccatttctcc ggatcagata
     4921 cggccgtgaa ctgctcccaa acctcctgct caagcgaaac gccctcgggg ctgtagcgca
     4981 ggacgatggg cgaacggccc tccatgatga tgtttggcga gcgtttcagc tctgctttgg
     5041 cgcgtgtcca ccatttggac cagctcttgg gctcgatgta gcgcggcacg agctcatagc
     5101 gaagctgatc ggcgtcgatg tgctgcccgt aagcgttaat gaggccgatc accagcgcga
     5161 ccgggtcgga ctcgatcagc tcgccgatgc gctccgggtg caactggcgg agcacgcgga
     5221 aatccttggg gtcgatgcgt tcgtattcgc ggaccagttc gcgcgccgga atggtggtga
     5281 cgcggtcggc gcgccgcagg gtgaacagtc cgcgcaggcg atccacctgg gcgacctcga
     5341 cgacgcgatc atccatgcgg ctgatgagcg tgtcgccgac ctcgagcgcc aggcagaagt
     5401 cgagcacgcg cagggcggcg cgcggcgcgg cgccggtggt cagacccgcg gattcgagaa
     5461 tggcgtcgaa gccgggcgtc tggccgtaga cgcggcgata cagatcgagc gtgagctggc
     5521 gcagttggtc actcttgggc gtggccaata gcgcggcgga ggcgaccttc agtgcctcgc
     5581 gcggttgggc ggcgacgtcg atgttctcga agatcatctg cgccagcggc gcgacacgtt
     5641 cggcctttcc ggtttgctcg atgagcgaga ggcattcggc cacttcggcc aggggcagat
     5701 taccgctgtc gagcaactcg aggcagcggg attcgaagtc gttcaggttt gcggctcgtg
     5761 ccagttcacg cagcttctgc ggcatcggat cctcaggaat cgtgtgtcaa agctcggcac
     5821 cgaccccatt ggcggcgccg ctcatccttg ttgatctttc tccaaatctt gacgcacccg
     5881 ctttttcgct tcgccggccg cgggtttcct gccggtcggg cgcgcgaggc catacgccgt
     5941 cgaggagggc gtttccaggc gcgccagcga agcgtggtcg aacgcggccg ggacagcgat
     6001 ttcgagcgtg ccacagcggg cgggggtccc gttggccatc tggtacttga aattaagtac
     6061 gtagttgccc cgccggctgg gcccagttcg cgcgagccgc agcgggctcg cctcgtgggt
     6121 gggcgaaacc agggcgccct gcacgttcat gaagttgacc acatccgcgt tcttgtcgta
     6181 cgcatcgatt accattttac cgcgccgggc acgaaccgtc gtgggccggt ccaggccggc
     6241 aacgggtact taggaggggg gtggcgcggc ggggtcgggc gagcgggcgg atcgatggtc
     6301 gaagcggata ctgcgcgcgt gcgagattgt tggcggattc ggggcgacac gccccggctc
     6361 tgctctctgc ggcgacacgc cctggccccg cgcgcgggac tggcggaacc tggcgctgcg
     6421 ccggtgaagg gtgcgattgg attcggtaca ctcccggctt tgccggctgc atccgggggt
     6481 cgccagacca cgcgtgagcc ggcgggtagg acgcgagatg cttaatcagc ctggttccga
     6541 tcacccgctg ccgagcttgt tttggatttg gccgttcgcc gcgcttttgc tggcgatcgc
     6601 cattctgccg ctggtccgcc ggacgcacca ctggtgggag cacaacgcca acaagctgct
     6661 ggtgtcattg gtgctggccg ccgtgacgct ggtctattac ggcttgcgcg gctacggcgt
     6721 cgagtcgcac gataaagaag cggcgccagc ggccgcgagc gctcccgccg cggcggacgc
     6781 cggccaaccc cagcttgagg cggagccgga agctcacgcg ccaacttata cggcgcgtgg
     6841 ctggccgacg gttcaggccg ttttgcggca cgcggttctg ggcgagtacg tcccgttcat
     6901 cgtgctgctg ttcagcctgt atgtgatcgc gggggggatc gtggtgcgcg gcgatattca
     6961 ggcaacgccc gcggcgaata cagcgattct cggcatcggc ggcgcgctgg ccagcatcat
     7021 cggcacgacc ggggcggcca tggttctgat ccggctgttg ctgaagacca acagcgagcg
     7081 gaagcgcaag gtccacacga tcgtcttctt catatttatt gtggcgaatg ccggcgggct
     7141 gctgttgccg accggcgatc cgccgctttt cctgggctat ttgtacggtg tgaactttct
     7201 ctggacgctg cgcctgtggc cgcaatggct gttcgtatta ggcgtgctgc tggtggtgta
     7261 ctttctctgg gacactcggg agtacgggcg ggagtcgccg gcggacctgg cgcgggacgt
     7321 cagcattgtg cgtccgattc gcgtagccgg attgctcaat atggtgtggc tggtcggggt
     7381 ggtggtcgcg gtgggcacgc tggaccccag caagccggtg cccggaacga actggtaccc
     7441 gtttccgtac ctgcgcgaga tggtgcagat ttcgctggtg gttatttcgg tgttgacgac
     7501 gcatgccaag ctgcgcgtgg agaatcagtt cacgtatgcg gcgattgggg aagtggcgtg
     7561 cttgttcatc ggcatattta tcaccatgca ggtgccgatc gagattttgc aggcgcgcgg
     7621 cccgcaactg gggctttccg cgccggcgca tttcttctgg gctaccggac ttttgagcag
     7681 cttcctggac aacgcgccga cgtatgcggt gtacttcgcg acggccgggg cgctgccgcc
     7741 gagcggcatg ccgctggttc aaggcgtgca gacggccacg ggggcgatcg cggcgccgct
     7801 gctggcggcg atcagttgcg gagcggtttt catgggggcg aatacgtaca tcggcaatgg
     7861 gcctaatttc atggtgcggg cgatcgcgga gcagtcgggc gtgcggatgc cgagcttctt
     7921 cgggtttatg atttactctg ggctggtgct gattccgctg tttgtagtga tgacgttcat
     7981 tttcttctgg agctgatggc tgcggtgtgg gcgggagcgc gaggcaacgt gaaatggcaa
     8041 agaaagcagc ccgcaagtcg agcgtaaaaa gggccgttgc gaaagcggtt caaaccgcag
     8101 aaggccggct ggcgaaggtc gaggccgcca aaggcggagc ggcaaaagcc caggcgcgca
     8161 aaggccggac cgccagggct caggccgcag tacgcaaagc ggccccgccg cggccggagg
     8221 cggtgcggac gggtgagagc caaacggcgc gtagtgcgcg ggcgttgcgc attctcaaga
     8281 tactccgcgc gacgtatccg gatgcgacga ctgccctgcg gcaccgcagt gcgtacgaac
     8341 tgctggtggc gaccatcctt tccgcccagt gcacggacga gcgcgtcaac caggtcacgc
     8401 ccgacctgtt tcgccgctat ccggatgcgg ccgcgctggc cgaggcggat ccggctgagt
     8461 tggaggcggg catcaagagc accggctttt tccgtaacaa gaccaagagt ctgctcggag
     8521 cggcgaagat gatccgcgac cgcttcggga accgcgtgcc cgacacgatg gctgagctgc
     8581 tggaactgcc gggtgtggcg cggaagacgg cgaattgcgt gctggggacg tggtatcaca
     8641 agaatgaagg ggtggtggta gacacgcacg tcgggcgggt ggctttgcgc ttggggctgc
     8701 tgactacggc acgcaatgac aaggatgccg tgaagatcga gcaggacctg atggcactgc
     8761 tgccgcgcga gagctggacg tttgtgtcgc acgccctgat tctgcacggg cgacgaatct
     8821 gcacggcgcg caagccaaag tgcggcgagt gcccgctgaa tgccgattgt ccgtcggcgt
     8881 ttaaggtgtg agggcaggcg gtttcggccc gcggttatac gatgtctgga cgcgtgccgc
     8941 agtgcgcacg ctcgcgcgtt tcacgggcgg ctctgccgct tatccgcgat tatggcggcc
     9001 gtcgccataa agactgcgcc gaccaagctt agcgtgaccg agcggccgac aacggacgcc
     9061 agtacgaacg ccagtaccgc caggatgctg gcggcgtaga acgcaacctc ccacgcgttc
     9121 cgtttcatcg gaaggggatt gccgggaggc ggcgtctctt ttttcgactt gatcaatcct
     9181 cacctcaccg cgccgcttgg ggacggcgtc gggatcagaa ctatgcagaa atcttcccta
     9241 aaagccagcc aagcgccccg tctaagtcgt catcgtccgc agtgacacag tagcccaacc
     9301 cccagccgat gtagatcgct gcttgcacta cagcagctcg gtttaagacg cgccctatcc
     9361 gatatctata ctcaaagttc aaggcagagg cggctgttat ggctgccgct acgttcgatc
     9421 tggggcggcc aagatgatgt cgtcgggccg aagagggcca aaagatcgct gaagccaggt
     9481 agcagtctgt ggaggttttt tctccgcaga tttcacagat ttgaacagat gtgcactcag
     9541 gtggctttcc acaacgggct cgctatgctc aagacaaccg cgaagacgcc gagagcgcta
     9601 aggccgccaa acgtcgcgcg ggatccaggg atcaaggcat cgaggggtca aggagcaggg
     9661 ccggggcgtg ccgccccgga gtaccgccac aggcctcggc atcgtcgtcg tactccacca
     9721 gcgtgtgcga aacggggacg cacatgtgtt cccgttttta accccgtttc tgactaagcc
     9781 agagtgcgcc gagcaacccg tccccgtttc acaccttgaa agcactgggc atgcacaacc
     9841 tccgtccgcc ctgcgggcgg aaacggaagt tattcagcgt ctaccaggga ctgcgtccct
     9901 ggcaacactc gcgcgccctc cgggcggaac cgaaggatgc cgcttgagtt aatggcattc
     9961 gggacagggg catctcagcg tgttaggcgc atttccaaga tgggcgcgcg cgtgagagtt
    10021 gcggggcgga gcgcattcga aggtgccata cccaagcgct aaagcgcggg catgccgtcg
    10081 gcagaagaag tcgggggcag aggcgaattg cggcgcgctg gcatcattcc ggggcggcac
    10141 gccccggctc tgctctgcga tgcgaggtta ctgccgatgg catgcccgcg cccgcaac
//
LOCUS       DAOSYS010000129        10193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5601_length_10193_cov_5.142434, whole genome shotgun sequence.
ACCESSION   DAOSYS010000129 DAOSYS010000000
VERSION     DAOSYS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_10193_cov_5.142434"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..1302
                     /locus_tag="PLU99_09215"
     CDS             61..1302
                     /locus_tag="PLU99_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17277.1"
                     /translation="MKLYAEISKTESQDDGTIKVWGYASSEAVDSDGETVTAEAMKAA
                     IPDYMKWGAVREMHQPSAAGTAIECRVEDDGRTLFGAHIVDSEAVKKVQANVYKGFSI
                     GGKVTSRDELNKSIIKGLKLVEISLVDRPANPEAVITVCKIDGDADQEPAVDALADLL
                     NKGAISAERLLELAKADLPAEEDLKKGLYTVTRLADVLDSLASICASSEWESQSEGDS
                     SPIPQALRDWLKEGAKILQDMAAEEVAEMLTDLQSLAQQSAARDLALADKTGDLAKAG
                     AKFSAAARDKLAKAHQAIREAGDHLASTGYDKDCDDEDGSKADAAADLAKVAGELDLA
                     KAEAVKIAAERDALQKRVQELEALPAPGKAFLRAVAKGEDAGAEDEPASKVAPVIDHR
                     GDINEAATLIKMIRAGAPVVK"
     gene            1393..2916
                     /locus_tag="PLU99_09220"
     CDS             1393..2916
                     /locus_tag="PLU99_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17278.1"
                     /translation="MGANTTAETLALLKVAQSNPDDIIKTFAAPGSATTGLQAYNLEA
                     PSLKLWPVLTPLRNSIARIGGGFATQANWKAITNINAGNTRAGIAEGKRGGTITHTLS
                     EYFAAFRGYGLENSVTFEAQYAGKGYEDLKALATLQTLQATMIQEERLILGGNTSVAL
                     GTTPTPSLVGSGSGGTLAAGTWSVIAVALSLQAYLDVVGVNNGAIGQSLAIASATVPG
                     QITRTNADGTTDTFGGGSAQKSAAATVVTTGSTSSIAATVANSGNGAVGYAWFWGAAG
                     SEALGAITSINSVSITAAATGTQLASSLTATDSSKSALDFDGLLTQAFKSGSNAYIAV
                     QATGTAGTGTPLTSDGAGGISEFEVAFVNFYNKYRTSPSTIYVSSQELVNITKKVIDN
                     GGAPLLKINANINAQTNGIAAGVVIGSYWNKVVGREIPLVVHPNLPAGTVFFYTAELP
                     YAVANVTAVARMLMRQDYYSLEWPLKTRKYEYGVYADGVLQHFAPFSMGIITNIGNG"
     gene            2989..3531
                     /locus_tag="PLU99_09225"
     CDS             2989..3531
                     /locus_tag="PLU99_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HOO17279.1"
                     /translation="MTTLANVKGWLGVTATTDDALLTRLISAASDYVQKWLNRTIAEQ
                     PYTETRDGTGGYVLPLSNYPVSAVSSLTIDGVSIPAAANAQGSGYLFTQTKLVLVGGY
                     RFWLGLQNVVVSYTAGYATTPNEIEQATIELISMRYKERDRIGQVSKSIGGETIAFSQ
                     KDFSEAIETTLSNYRKVILL"
     gene            3528..4022
                     /locus_tag="PLU99_09230"
     CDS             3528..4022
                     /locus_tag="PLU99_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HOO17280.1"
                     /translation="MIEGYLVGDREAVARFSHFGPELQSQLVKRVTRLAFALQKRVQE
                     TKLSDQVLHVRTGRLRRSINSRVEEDGARVYGFVGTNVVYGQRHEMGFSGTENVKAHS
                     RLMTVAFGRKVKDPRQISVRAFSRQIKYAARPFLRPALAEMKPEIVKGMEQALRDAAK
                     VAFT"
     gene            4019..4444
                     /locus_tag="PLU99_09235"
     CDS             4019..4444
                     /locus_tag="PLU99_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17281.1"
                     /translation="MIARETIYAALFAKLSAVAGFVTTSRRLKHWDDVPQQQQPALFL
                     SQHTETLTTSPGGDRHWQFSLIALIYVHTQADKTIAPTTLLNPIIDSVVAALLPDAIT
                     KRNTLGGVVMDAQISGAIEIDHYDDQAVVAIPITIKAAQ"
     gene            4455..5207
                     /locus_tag="PLU99_09240"
     CDS             4455..5207
                     /locus_tag="PLU99_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17282.1"
                     /translation="MGYAYMGSGKIRIAPYSTSNTYEQRAFVDVENASIFEITLQPDE
                     KELMDFTNPAGGTDASVKRIKTVTGKMDLRHFTAANLARAMWGTTTAQTATPITGEAG
                     YKINAGGFIATKRLIDITVAPVLKKGGTAILSADFTYSAGGVQIASTITTPAVTSGDA
                     ITIDYTPLLSTSAHGLQAVAPLVSIHFEGIDVNSGKQLAAKIWQAKLGSAQSLSLIGD
                     EFGVLSLPFMALKDSTISAATNSQFCEIQIAS"
     gene            5207..5533
                     /locus_tag="PLU99_09245"
     CDS             5207..5533
                     /locus_tag="PLU99_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17283.1"
                     /translation="MALTSQVHLGPRVVTVRELTFADVRDWLRRREILKSCDVIHALA
                     FEDFGIDDLAEMSDATTEEMEACAPSELKPLIEACKALNPHFFRTRAALAQAARATLA
                     NAAQQT"
     gene            5728..5859
                     /locus_tag="PLU99_09250"
     CDS             5728..5859
                     /locus_tag="PLU99_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17284.1"
                     /translation="MSGILLESALIELVTLDKPMELIISKHRLPDGSYHFGVTLLDR"
     gene            5871..6218
                     /locus_tag="PLU99_09255"
     CDS             5871..6218
                     /locus_tag="PLU99_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOO17285.1"
                     /translation="MLRAFLLACLLLPFSAVAQYKCVIDGRTSYTERPCAPGAKPLEL
                     KTDIPITDAERSRAQAENWKRHAELFKAIGRSARVTGADLSAVDRMARYEKDIGDEEA
                     RRQEVEAARKRQK"
     gene            6276..>7342
                     /locus_tag="PLU99_09260"
     CDS             6276..>7342
                     /locus_tag="PLU99_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17286.1"
                     /translation="MADDIVDVKFGGDAGPAKKAIEDTRAALKSASADMSASLKSLKA
                     PVTEFQAALAGLASVLAGGAMFKASVEAANDWNKEITKLSKAMGTTAEEASVMAVALK
                     HIGLDADAVTSASLAMTRQLSHNEAAFQAFGVQTRDVQTGALKPIGDIMASVNQKLLA
                     QTNVAQRNADGMRFYGRQWTEVMGILKLTTQAMEEANARAKELGLIVGQEGVQATREL
                     KEEQRDMGLILKSVEIQIGEKLLPTLVKLGSYLVGEGAKAVSGFGTLLDWLSKGILSL
                     VVVWQQMATAAAFGASVVKAALTGNLSEVRRLYEAYAELSDEHAAAMRKLWDDDQKAA
                     ASAEKLAQQKKKLETDLQIQLS"
     assembly_gap    7343..7442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7443..8596
                     /locus_tag="PLU99_09265"
     CDS             <7443..8596
                     /locus_tag="PLU99_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17287.1"
                     /translation="KLAQQKKKLETDLQIQLSKLEDLRVQAAKGANAEILKSDKERTD
                     EQLKDAQRLHQALQKAFEDSVRSAKSAKDEAAKLLQSAADKAQSGADAAAATRERLLS
                     PDDQKIVRQNREDKAGREIDEAGRMVGIASVAVYAGRNESAKKLLDTNEKILEAQKKI
                     VDAMEDSEEKARRQESIGNHLSQIDTARARMKEGEAKTEEERTQALADKLKEVEAQIK
                     ELTDAPAKVKIEADIQQAIDGINTTLVALGQLQDKTVTVTVKTVQEGQPVAASGGGSD
                     APPGFAEGGYTGSTGGIVHPYEQVIRADVVAQPGVRAMLETLNRGGVSALASAGMVNA
                     PAAAPASSGSPNVFNFPGMGSFEATMAPDQQAALEVAFRRVALQKGARR"
     gene            8593..9108
                     /locus_tag="PLU99_09270"
     CDS             8593..9108
                     /locus_tag="PLU99_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17288.1"
                     /translation="MKALIIGGVSIPDLAALELQQTYEPIGGELMLRTIGGSAIKQIT
                     WQKWRTTITGGGWLPFGLSSLDYSAQMSVACIVPRAVVCSGLSATLPAARRSDTGYLP
                     WGVALLADQTTQVTAATMAGNVATLDAVAGAIGYHAMYYPLLQCYVSRPKEQGVRETA
                     SYGWSIVAEES"
     gene            9111..>10193
                     /locus_tag="PLU99_09275"
     CDS             9111..>10193
                     /locus_tag="PLU99_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17289.1"
                     /translation="MPATYSGTGSTSTAGVWTAIVTVDGSDVSSRVVGEIRIDAEESS
                     ARTAEFTIRPAAGTSFAVANWVGKSVTIDIADNASGSPANVQRLFTGLIDTPTLNLVH
                     RTIAILCTDDLQGKIDAMSNSAIDAAIPSSYYSNVIFNSAETGWRRAQDRLSTLASAL
                     DLDPSGSLRLTAWAAKVTPDLSFTNAHILDESPGLALASRTALINSIEIDFGYRFPRV
                     KAEGYPITYSYVTSSTIAAHVAAGNWFLLRSSVEAAIKAAGGTIQSISYDALPNTTIG
                     TFTPGPYDSLSCMGFTAVVSFDYAQQIEEQYTITVSAPNSIAAVGTLPDKLQGALEGV
                     YPQVVAAENSMTLYRNDVSGIPPMDLA"
BASE COUNT         2165 a         2967 c         3199 g         1762 t
ORIGIN      
        1 ggcctgatcg cgtcaaacag accaggccag atccgcgcat aactccagga gaatcagcaa
       61 gtgaaactgt acgcagaaat cagcaaaacc gaatcgcagg acgacggaac gatcaaggtc
      121 tggggttacg catcgagcga ggctgttgat tcggacggcg agacggttac agccgaggcc
      181 atgaaggccg ccatccccga ttacatgaaa tggggagccg tgcgcgagat gcaccagcca
      241 tccgccgccg gaaccgccat cgaatgccgg gtggaagacg acggcagaac gctcttcggc
      301 gcgcatatcg tcgattcgga ggcggtcaag aaggtgcagg cgaacgtcta caaggggttc
      361 tccatcggtg gcaaagtgac cagtcgcgac gagctgaaca agtcgattat caagggactc
      421 aagctcgtgg aaatctccct tgtagatcgc ccggccaatc ctgaagcggt catcaccgtt
      481 tgcaagatcg acggcgacgc ggatcaagag ccggcggtcg atgcgctggc cgatctgctc
      541 aataagggcg ccatttccgc agagcgattg cttgagcttg cgaaggccga tctgccggca
      601 gaagaagacc tgaagaaggg cttgtacacg gtcactcgac ttgccgatgt gctggattcg
      661 ctggcaagca tttgcgccag cagcgaatgg gagtcgcaga gtgaaggcga ttcttcgccg
      721 atcccgcagg cgctgcgcga ctggctgaaa gagggcgcga aaatcctgca ggacatggcc
      781 gccgaggaag ttgccgagat gctgacggac ctccaaagct tggcgcagca atcggcggct
      841 cgggatctgg cgctcgccga caagaccgga gaccttgcca aggcgggggc gaagttctct
      901 gcagccgctc gcgacaagct cgccaaggcg catcaggcca tccgcgaagc tggcgaccat
      961 cttgccagca caggatatga caaggactgc gacgacgaag atggcagcaa agcggacgct
     1021 gctgccgatc tggcgaaggt ggccggcgag ctagatctgg cgaaggccga agccgtcaag
     1081 attgccgcag agcgcgacgc actgcagaag cgagtgcaag agctggaagc cctgcccgct
     1141 cccggcaagg catttctccg cgcggtggca aagggcgaag atgccggcgc ggaagatgag
     1201 ccagccagca aggttgcccc tgttatcgac catcgaggcg acatcaacga ggccgcgacc
     1261 ctcatcaaga tgatccgcgc gggcgcgcct gtcgtcaagt aatcgatttt tcaacaatcc
     1321 ggccgcgttg cggccttttt catgtttgcc attcgccgcc attgaggcgg cttttttttg
     1381 gagcaaaaca ccatgggcgc aaataccacc gccgaaactc tcgcgctgct gaaggtggcg
     1441 caatcgaatc cggatgacat catcaagacg tttgccgcgc cgggcagcgc gacgaccggc
     1501 ctgcaggcgt acaacctcga agccccgtcg ctcaaactgt ggcccgtcct tacgccgctg
     1561 cgcaacagca tcgcgcgcat cggcggcggg ttcgcgacgc aagcgaactg gaaggcgatc
     1621 acgaacatca acgccggcaa cacgcgcgcc ggcatcgccg aaggcaagcg cggcgggacc
     1681 atcacgcaca ctttgtccga atacttcgcg gcttttcgcg gatatgggct ggaaaacagc
     1741 gtgacctttg aagcgcagta cgccggcaag ggttacgaag acctcaaggc gttggcaacc
     1801 ctgcagacgt tgcaggcgac catgatccag gaggagcgct tgatcctcgg cggcaatacg
     1861 agcgtcgcct tgggaaccac gccaacgccg tcgctggtcg gatcgggctc tggcgggacg
     1921 ctggccgctg gaacgtggag cgttatcgcc gtcgcgctgt cgctgcaagc gtatctcgat
     1981 gtcgtcggcg tcaataacgg ggccatcggg cagtccctcg ccatcgcatc ggcaacggtg
     2041 ccgggacaga ttacgcgcac caatgcggac ggaacgaccg acacatttgg cggtggctcg
     2101 gcgcagaagt ctgcggcggc tactgtcgtg acgaccggat cgacttctag tatcgccgct
     2161 accgtagcca actccggcaa tggagcggtc ggttacgcat ggttctgggg tgccgccggt
     2221 agtgaggctc tgggcgcaat cacgagcatc aacagcgttt cgattacggc tgccgctact
     2281 ggtacgcaat tggcaagctc gctgacggca acggattcga gcaagtcggc gctcgacttt
     2341 gacggactgc tcacgcaggc attcaagagc ggatcgaatg cgtacatcgc cgtgcaggcg
     2401 accggcaccg ctggcaccgg cacgccgctg acttcggacg gcgcgggcgg gatctcggaa
     2461 ttcgaggtgg cgttcgtcaa tttttacaac aaatatcgca catcgccgtc cacgatctac
     2521 gtcagttcgc aggagttggt caacatcacg aagaaggtca tcgacaacgg cggcgccccg
     2581 ctgctgaaga tcaacgccaa catcaacgcg cagacgaacg gtatcgctgc cggcgtcgtc
     2641 atcggaagct actggaacaa ggttgtcggc cgcgagattc cgctggtcgt gcatccaaac
     2701 ttgccggccg ggacggtctt cttctacacc gcagaattgc cgtatgcggt cgccaacgtc
     2761 accgccgtcg cgcgcatgct gatgcggcag gactactact cgctcgaatg gccgctgaag
     2821 acgcgcaagt acgagtatgg cgtctatgcc gacggtgtgc tgcagcattt cgcgccgttc
     2881 tcgatgggca tcatcaccaa catcggcaac gggtaatcca gtctcttcca ggtgtgaaag
     2941 ggccgctttc gggcggcctt ttcttttggg gaatcacgca tggctgattt gacgacgctg
     3001 gcgaacgtca aagggtggct cggcgtcacc gcgacaaccg acgacgcctt gttgacgcgg
     3061 ctgatttcgg ccgccagcga ttacgttcag aagtggctta accgcacgat cgcggagcag
     3121 ccgtacaccg agacgcgcga cggaactggc ggctacgtgc tgccgctgtc caactatccg
     3181 gtatcggccg tgtcttcgct gacgatcgat ggtgtttcga ttcccgccgc agccaacgcg
     3241 caaggttccg ggtatctatt cacccagaca aagctggttc tcgttggcgg ttatcgattc
     3301 tggctcgggc tgcagaacgt tgtcgtaagt tacacggctg gctatgccac tacgccaaac
     3361 gaaatcgagc aggcgacgat cgaactaatc tcgatgcgtt acaaggagcg cgaccgcatc
     3421 gggcaagtca gcaaatcgat aggcggagaa acgattgctt tctcgcagaa ggatttttct
     3481 gaagctatcg agacgacgct atccaattac agaaaggtca tcctgctgtg atcgaggggt
     3541 atctcgtcgg cgatcgcgaa gcggttgcgc gattctccca tttcggcccc gagttgcagt
     3601 cgcaactggt caagcgcgtc acccgtctgg cgtttgccct gcagaagcgg gtgcaggaga
     3661 ccaagctatc agatcaggtg ttgcacgtgc gcaccggcag gctgcgccgt tcgatcaatt
     3721 cccgcgtcga ggaagacggc gcgcgcgtct atggcttcgt cggcacaaat gttgtctatg
     3781 ggcagcgtca cgagatggga ttttccggaa cggaaaacgt caaggcgcac tctcgcctga
     3841 tgacggtggc ctttgggcga aaggtcaagg accctcggca aatttccgtc cgcgcgttct
     3901 cgaggcagat caagtacgct gcgcgtccgt tcctgcggcc ggcgctcgcc gagatgaagc
     3961 cggagatcgt caaaggcatg gaacaggcgc tgcgtgacgc cgccaaggtg gccttcacat
     4021 gatcgctcgc gagaccattt atgcggcgct gttcgccaag ctgtcggccg ttgccgggtt
     4081 cgtcacgaca tcgcgccgac tcaagcattg ggacgacgtg ccgcagcagc agcaaccggc
     4141 gctgtttctg agccagcaca cggagacgct gaccacgagt ccgggcggcg atcgccattg
     4201 gcaattttcg ctgattgcgc tcatctacgt gcatacgcag gcagacaaga cgattgcccc
     4261 gacgacgcta ctcaacccga tcattgattc cgttgtcgcc gcgctgttgc cggacgccat
     4321 caccaagcgc aacacgctcg gcggcgtggt catggacgcg cagatcagcg gcgctatcga
     4381 aatcgatcat tacgacgacc aggcggttgt cgccatcccc atcaccatca aagcagcaca
     4441 ataggaggca tcacatgggt tatgcataca tggggtctgg aaaaatccgg atcgccccgt
     4501 acagcacgag caacacttac gagcagcgcg ctttcgtcga cgtcgaaaac gccagcattt
     4561 tcgagatcac cctgcagccg gacgagaagg aactgatgga cttcacgaac ccggcaggcg
     4621 gcacggacgc cagcgtgaaa cgcatcaaaa ccgttaccgg aaaaatggac ttgcgccact
     4681 tcacggcggc gaacttggcg cgcgccatgt ggggaacgac gacggcgcaa acggcgaccc
     4741 cgataaccgg cgaggcgggc tacaagatca atgcgggcgg cttcatcgcc acgaagcgat
     4801 tgatcgacat caccgtagcc cctgtgctga aaaagggagg gacggccatt ctgtcggctg
     4861 atttcaccta ctctgccggc ggcgtccaga ttgcgagcac gatcactacg ccggccgtga
     4921 cttccggcga cgccatcacc atcgattaca cgccgctgct ctcgaccagt gcgcatgggc
     4981 tgcaggcggt tgcgccgctg gtgtcgattc atttcgaggg catcgacgtg aattcgggca
     5041 aacaactggc ggcgaagatc tggcaggcca agttgggcag cgcgcagtcg ctgtctctga
     5101 tcggggacga attcggagta ctgtctttgc cgttcatggc gctcaaagat tcgacgattt
     5161 cagcggcgac caactcgcaa ttctgcgaaa tccagatcgc gtcctgatgg ctctgacgag
     5221 tcaagtccat ctcgggccgc gcgtggtcac ggtccgggag ctgacatttg cagacgtgcg
     5281 cgactggctg cggcggcgcg aaatcctcaa gtcctgcgat gtgattcacg cgctggcatt
     5341 cgaggatttc ggcatcgatg acctggcgga aatgtcagac gcgacgaccg aagaaatgga
     5401 ggcttgcgcg ccgtccgaac tcaagccgct gatcgaagcc tgcaaggcgc tgaacccgca
     5461 ttttttccgc acgcgggcgg ccctggccca ggccgcccgc gccacgctgg ccaatgccgc
     5521 gcagcagacc tagaccggtc cgcgtgtatc cttgtgacgc acgggcatgc gggggtgtgg
     5581 ggttacacgt ggagcgcata tctcgccgct gttgcgctgg caaatgagtc aacccagtcg
     5641 gccaaggcgc gttagaatgc ctgagtgggg acagatcaac cagacgaccg accgattgcc
     5701 aaaggttcgc gctattggct tccgggcttg tcgggcattc tgttggaatc ggcgctgatt
     5761 gaactggtca cactcgacaa gccgatggag ctgattatca gcaagcaccg attgccggac
     5821 ggctcctatc acttcggggt aacgctgctc gaccgttgag gattgccgcc atgctgcgcg
     5881 cctttcttct tgcctgtctc ctccttcctt tttcggccgt ggcgcaatac aagtgcgtga
     5941 tcgatggccg cacgagctac accgagcgcc cctgcgcgcc gggcgccaag ccgctcgaac
     6001 tcaaaaccga catccccatt accgacgccg agagaagccg cgcgcaggct gaaaactgga
     6061 aacgacatgc ggagctgttc aaggccatcg gccgcagcgc gcgcgtaacc ggcgccgact
     6121 tgtctgccgt cgatcgaatg gcgcgatacg agaaagacat cggcgacgag gaagcgaggc
     6181 ggcaagaagt ggaagccgcg cgcaagcgcc aaaagtagcc tcgtcaagaa cgaacaccgc
     6241 gccgccttcg ggcggctttt ttttgggcaa attccatggc tgacgacatc gtcgacgtca
     6301 aattcggcgg cgacgccgga ccggcaaaga aagcgatcga ggacacgcgc gccgcgctca
     6361 agtcggcgtc ggcggatatg tctgccagtc tgaagtcgct caaagctccg gtaacggagt
     6421 ttcaggccgc gctggccggc ttggcgtccg tgctggccgg cggcgccatg ttcaaggcgt
     6481 ccgtagaagc ggccaacgac tggaacaagg aaatcaccaa gctttccaag gcgatgggga
     6541 cgaccgccga ggaagccagc gtgatggcgg ttgcgttgaa gcatatcggc cttgatgcgg
     6601 acgcggtaac gtcggcgtcg cttgccatga cgcggcaact gagccacaac gaggcggcgt
     6661 tccaagcgtt cggcgtgcaa acgagggacg tgcaaaccgg cgcgctgaag ccaatcggcg
     6721 acatcatggc gagcgtcaat caaaaactgc ttgcacagac caacgttgcg cagcgcaacg
     6781 cggacgggat gcggttctat ggccggcagt ggacagaggt catgggcatc ctcaagctga
     6841 ctacgcaggc gatggaagag gcgaacgcgc gcgccaagga actcggtttg atcgtcggtc
     6901 aggagggagt gcaggcaacg cgggagctga aggaagagca gcgcgacatg gggcttatcc
     6961 tcaagtcggt tgagattcag attggtgaaa agctcctgcc aaccctcgtc aagctcggtt
     7021 cgtatctggt cggcgaaggc gcaaaagcgg tgtccggctt cggcacgctg ctggattggc
     7081 tatccaaggg cattctgtcg cttgttgtcg tttggcaaca gatggcgacg gcggcggcat
     7141 ttggcgcatc cgtggtcaag gccgcgctga ccggcaacct gtctgaggtc aggcgcctgt
     7201 atgaagccta tgcagagctt tccgacgaac acgcggcagc catgcgcaag ctgtgggatg
     7261 acgaccaaaa ggccgccgcc agtgccgaaa agcttgcgca gcagaaaaag aagctcgaaa
     7321 ccgacctgca gatccagtta tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnaaaagctt gcgcagcaga aaaagaagct cgaaaccgac ctgcagatcc agttatccaa
     7501 gctggaagat ctgcgcgtgc aggcggccaa gggagcgaat gccgagatcc tgaagtccga
     7561 caaggaacgt actgacgagc aactgaagga cgcgcagcgg ctgcaccaag cgttgcagaa
     7621 ggcgtttgag gattcggtgc ggtcggcaaa gtctgccaag gacgaagctg ccaagctgct
     7681 gcagtctgca gccgacaagg cgcagagcgg ggccgatgcg gcggcggcga cgcgcgaacg
     7741 gctgctcagc ccggacgacc aaaagatcgt gcggcaaaac cgggaggaca aggccgggcg
     7801 ggaaatcgac gaggccggcc gtatggtcgg aatcgcaagc gtcgcagtct atgccgggcg
     7861 caacgaatcc gccaagaaac tgctcgacac gaacgagaag attcttgagg cgcagaagaa
     7921 aatcgtcgac gcgatggaag acagcgaaga gaaagcgcgc cgtcaagagt cgatcggcaa
     7981 tcacctgtcg cagatcgata ccgcccgcgc gcgcatgaaa gagggcgagg cgaagacgga
     8041 agaagaacgc acgcaggcac tcgccgacaa actcaaagag gtcgaagcgc agatcaaaga
     8101 attgaccgac gctccggcga aggtcaagat cgaggcggac atccagcagg caatcgacgg
     8161 catcaatacg acgctggtgg ctctcgggca gttgcaggac aagacggtaa cggtaacggt
     8221 caaaaccgtg caggaagggc aaccagtcgc cgccagcggt ggcggatcgg acgcgccacc
     8281 gggctttgct gagggcggct acaccggatc gacgggcggg atcgttcatc catacgaaca
     8341 ggttatccgg gctgacgtgg tggcgcagcc gggcgtgcgc gccatgctcg aaacgctcaa
     8401 ccgtggcggg gtgtcggctc tggcctcggc gggaatggtc aatgctcctg cggcagcccc
     8461 ggcatcgtcc ggcagtccca acgtgttcaa ctttcccggc atgggcagct ttgaggcgac
     8521 gatggcgcca gatcaacagg cggcgcttga ggttgcgttc cggcgtgtcg cgctgcagaa
     8581 gggggcgaga cggtgaaagc gctgattatc ggtggcgtgt cgattccaga ccttgccgcg
     8641 ctggagctgc agcagactta cgagcctatt ggcggcgaac tgatgctgcg taccatcggc
     8701 ggatcggcaa tcaagcagat tacatggcaa aagtggcgca cgacgattac cggcggcgga
     8761 tggcttccgt ttggcctgtc gtcgctcgac tactccgcgc agatgtccgt agcgtgcatc
     8821 gtgccgcgtg ctgtcgtttg ctccgggttg tcggcaacgc ttccggccgc tcggcgcagc
     8881 gatacgggat atctgccgtg gggcgttgcg ctactggccg accaaacgac acaggtaacg
     8941 gcggcgacga tggccggcaa tgtggcgacg ctcgacgcgg ttgccggggc aatcggatac
     9001 catgccatgt actaccctct cctgcagtgc tacgtcagcc ggccgaagga gcagggcgtt
     9061 cgtgagacag cctcttatgg ctggtcgatc gtcgccgagg agtcgtaatc gtgccagcga
     9121 cctatagcgg gaccggcagc acgagcactg cgggcgtatg gacggcgatc gttaccgttg
     9181 acggcagcga cgtgtcatcg cgcgtggtcg gagaaattcg catcgacgcc gaggagtcgt
     9241 ccgcgcggac ggcagaattt accattcgcc cggccgcagg aacctcattt gccgtggcga
     9301 actgggtagg gaaatcggta acgatcgaca tcgccgacaa tgccagcggg tcgccggcca
     9361 acgtgcaaag gcttttcact ggccttatcg acacgccgac gctcaacctc gtgcacagga
     9421 caattgcgat tctgtgcacc gacgacctgc agggcaaaat cgacgcgatg agcaattcgg
     9481 ccatcgacgc agccattccg agcagctact acagcaacgt gatattcaat tcggcggaaa
     9541 cgggatggcg acgggcgcag gaccggcttt ctacgctggc ctctgcgctc gacctcgatc
     9601 cgtccggaag tctgcgcttg acggcatggg cagcgaaagt gacgccggat ttgtccttca
     9661 ccaatgcgca tattctcgac gagtcgcccg gcttggcgct ggcttcgcgg acggcgctga
     9721 tcaactcgat agagatcgat ttcgggtatc gctttccgcg cgtcaaggcc gagggatacc
     9781 cgatcacgta cagctacgtg accagcagca cgatcgccgc gcacgtcgct gccggcaatt
     9841 ggttcctcct gcgctcgtcg gttgaggcgg ccatcaaggc ggccggcggg acgattcaaa
     9901 gcatcagtta cgacgcgctg ccaaacacga ccatcgggac tttcacgccg gggccttacg
     9961 actcgctctc atgtatgggt ttcactgccg tcgtcagttt cgactacgcg cagcagatcg
    10021 aggagcaata caccatcacg gtgtctgcgc cgaacagcat cgccgctgtc ggcacgctgc
    10081 cggacaagct gcaaggggcg cttgagggcg tctatccgca ggttgtcgcc gcagaaaatt
    10141 cgatgacgct ttaccgcaac gacgtaagcg gtatcccgcc gatggatctg gcg
//
LOCUS       DAOSYS010000130        10189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5605_length_10189_cov_5.033353, whole genome shotgun sequence.
ACCESSION   DAOSYS010000130 DAOSYS010000000
VERSION     DAOSYS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5605_length_10189_cov_5.033353"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..1137
                     /gene="folE"
                     /locus_tag="PLU99_09280"
     CDS             469..1137
                     /gene="folE"
                     /locus_tag="PLU99_09280"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007466119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOO17290.1"
                     /translation="MADIAPNRQPRGNTDPRRTAKRVGPSGDGRTAPAARKLIDIPTI
                     ERAFREILIAIGEDPDREGLRKTPARIARMYAELFAGLRQDAEEHLQATFDEGHHEMV
                     VLRDIPFNSMCEHHFMPFEGKAHVAYIPGGKVLGLSKLARIVDVYSHRPQVQERLTSQ
                     VADLLAKRLQVKGCAVVLEAVHTCMTCRGVKKSGSVMITSALRGIMQTSQSTRAEALS
                     LLNR"
     gene            1359..1742
                     /gene="gcvH"
                     /locus_tag="PLU99_09285"
     CDS             1359..1742
                     /gene="gcvH"
                     /locus_tag="PLU99_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOO17291.1"
                     /translation="MPVPSDRKYSQTHEWFKADGKIVTMGISKFAADELTDVTYVSLP
                     QVGSQIEGGTHFGEVESVKATSELFTAVSGTVREVNPRLADEPELVNNDPFGEGWMLK
                     VECTDLSPLDKLLDAAAYDKMISEA"
     gene            1848..4772
                     /gene="gcvP"
                     /locus_tag="PLU99_09290"
     CDS             1848..4772
                     /gene="gcvP"
                     /locus_tag="PLU99_09290"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOO17292.1"
                     /translation="MSTDSNLPDLLGPSDSFVDRHVGPRDAEIREMLGLLGYRSLDEM
                     IDATIPAAIRLKKPLNLGPPRGEREALEELRGIARQNQLFRSLIGMGYYGTITPPVIL
                     RNVLENPGWYTQYTPYQAEISQGRLEALMNFQTMVCDLTALPLANASLLDEATAAAEA
                     MTMCARVSRGKSKCFFVAQDCHPQTIAVVQTRARPLGFEVVVGDPLKTDFSKQACFGA
                     LLQYPTTDGRVECYEKTIEQVHAAGGLAVVATDLLALTLLKPPGEFGADIAIGSSQRF
                     GVPMGFGGPHAAFMATRTEYTRQMPGRLIGMSKDADGGMGFRLTLQTREQHIRREKAT
                     SNICTAQVLLAIMAGMYATYHGPAGLRRIGSRVFALTHALRTGLRQLGHEINDAHVRF
                     DTLRVKPVGRTAGDVIRAARERRINLRDYGDGTVGVSLDETTDVALVQDVLAAFGGRG
                     DEDVVALAGSPDKLIPAALRRTSPYLSHPVFNSYHAEHEMLRYIKRLESRDLSLCQSM
                     IPLGSCTMKLNAAAEMLPITWPEFAYLHPFAPVEQAAGYIELFRQLEGWLAEITGFAA
                     VSLQPNSGAAGEYAGLMVIRAYHQARGEGHRNVCLIPVSAHGTNPASSAMAGYQVVPV
                     ACDEHGNIDVADLKRRAEENRDKLAALMITYPSTHGVFEEAVRELCQIVHANGGQVYM
                     DGANMNAQVGLCRPGDIGADICHLNLHKTFSIPHGGGGPGVGPIACAEHLKPYLPGHP
                     VVNPAVGRWECGKAGTACCSDAGRVGAVAAAPFGSPSILPISWMYIRMMGEPGLKKAT
                     QVAILNANYMAHRLREHYPIYYTGKNGRVAHEFILDCRELKKAHVEVEDIAKRLMDFG
                     FHAPTMSWPVHDTLMIEPTESESKQECDRLVEALIAIKAEAQAIAEGRMDAENNPLKN
                     APHTARAVTATEWNHPYSREQAAFPTEWLRHYKFWPPVARIDNVHGDRHLCCACPADW
                     GA"
     gene            5107..5316
                     /locus_tag="PLU99_09295"
     CDS             5107..5316
                     /locus_tag="PLU99_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOO17293.1"
                     /translation="MKYGARNQLRGEVVEIKKGTVMCEVKVRVTAPAQMASVMTLESL
                     EELGLKQGDQVNVVVKAVNVLLTKD"
     gene            5410..7446
                     /locus_tag="PLU99_09300"
     CDS             5410..7446
                     /locus_tag="PLU99_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17294.1"
                     /translation="MASPSSTDVRAEAGGVKRSVGAGPKRRPPARGQQTFALPAGGWS
                     ERRFWLILGLICLVALICRLAILVEYTARNPLAASPINDAQTYWDWAERIAQGKLIQD
                     TPFFSAPLYPYLLGLIRAAGGSLRLIYLLQILADLATACLLAYAARGRFGARVGLLAA
                     ALFLLLEEPASFSLRILTCSLQLLLLAITYLLMLRAQDAMTARRAGASQAVTIPEGTL
                     SDSGTAVSWIPARFAPALLGVALGFLCLSYAPAILLVVAVVPWLYWQSGRTRRDVLPA
                     LTPLAVATLLIAPATLHNWYTSGDFFLIQSVTAVNLRQGNQPQSIGVYTPIPDTTIGR
                     ERLFDDVARQYELVHGRRGTWAEIDRYYRDLVWKFWADDPGRAVKLAARKFYYFLTAR
                     DYGDIYQPQAELRAGMDRLFWLTPLPLAWLIGPALVGFVLLLRRPIRFAPELLMFLIP
                     LIVVTVHWYTPRYRLPAMLAVVVVAAWVFDTALRWRVQGLRAVGLGAVAAAALGAEML
                     LGPINRAAGIDVCDPAGVMFHVGGVLGQQGKPEDAAALFRRGLQIRPNDPLARVDLGD
                     VLLDLGRRDEALAEFEHARQFAPDHPELLGRIAMLLMERQRLPEARAALERALPRDPR
                     NPVLLRLAADCYQRILRADPNDAAARNSLNWVEQQFRTGRPGGRPEAIRASERP"
     gene            7534..7618
                     /locus_tag="PLU99_09305"
     tRNA            7534..7618
                     /locus_tag="PLU99_09305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7568..7570,aa:Ser,seq:tga)
     gene            7978..9510
                     /locus_tag="PLU99_09310"
     CDS             7978..9510
                     /locus_tag="PLU99_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17295.1"
                     /translation="MRISRTMLGLFIATILFVLTLAARGAAQPAYRAIELGTTGGDQS
                     EAYAINDYAEIVGRSFDATGEAQVFYWQNGQILALFHEMPWGRIPMPATRYSVGYGMS
                     NTGYVITYVMDESEDDPGLLVPSAVIFRPTLSDLTTPYQAQAITYIGTLCSPTGISSS
                     GRYVVGWGQLDCSSRAEAFLVTPMNGTWAVPDGTCVNNALVIGLGTLEALDMVSTATA
                     VNNHGQVVGWSYSDTGGYQAFLINPTVDVDGNPVAWFTDDGEGNNALMTPLGTLPGTG
                     GESFGTNSLARDINDAGLIVGEADTGDYVTHAFLYANGAMLDLGTLGGPASSACAINE
                     NGQIVGWALNAAGLPRAFVIIPADADDDGAPDTWYADEDEDGINDLMIDLNHVVNSGF
                     ALQLTEARDVNIHGQIVGWGTSGTGDLALRLAFLLTPAAPANGNNDNGDSGPVLGDVN
                     LAPLLSDPAEQPEQPGENPQAPAPTPSLGLAHWLCGVGMVQLLPLMLAGLWAMKLSFR
                     RR"
     gene            9670..>10189
                     /locus_tag="PLU99_09315"
     CDS             9670..>10189
                     /locus_tag="PLU99_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOO17296.1"
                     /translation="MFDALSEKLTGVLRSLRGRGRITEDNVREAMREVRTALLEADVH
                     IEVARKFCDECQQKALGMQVLETLRPEQVMIKVVHDELVRLMGPIETRIPFVSQPPTI
                     ILMAGLQGSGKTTTCAKLARYCLQKGKKPMMVAADLKRPAAIDQLEVLGTQVGVPVYV
                     ERDHQNAVKVCRN"
BASE COUNT         1827 a         3124 c         3444 g         1794 t
ORIGIN      
        1 tgcatgcctc cgggcccgcg tgcccgagct atatcatcta gaaaagatcg gccccgcgcg
       61 cagaaaggta ttgactatgc ggctttcgaa atgtgccccc tccttacctc tcacctacca
      121 actctctccg cgcagccgcg cctcaggtta tcaaaatcga gtgcacaaga accatgagtg
      181 cctgatcatc tgcccccgtg gcccgatccc acacgctttt ttatcagacc gcttgatcac
      241 ctggggtaag gccacgcagc gagttatcgg cactttgcca aacccgaacg cgcaacctcg
      301 ggctctaccg gacgggcgcg gaccgcgggg tgggacggcg cgaatgcgaa ggcctttggc
      361 ggtactccag ggcagcacgc cccggctccg ctctccacgc ctgcttccaa tgacaacgcg
      421 ctcagctcgc gtacaatacc gtacccgaat catcggaacg gagacgccat ggcggacata
      481 gcacctaaca ggcaaccccg cggtaacacc gacccccgcc ggactgccaa acgagttggt
      541 ccaagcgggg acggacggac ggctccagcg gctcgcaagc tgattgacat tccgaccatc
      601 gagcgtgctt tccgggagat tctgatcgcg atcggcgaag atcccgaccg cgaaggcctg
      661 cgcaagacgc ccgcccgcat cgcccgcatg tacgcggagc tcttcgcggg tttgcggcag
      721 gatgctgaag agcatctgca agccaccttc gacgagggac accatgagat ggtggtgctg
      781 cgcgacatcc cgttcaacag catgtgcgag caccacttca tgcccttcga gggcaaggcc
      841 cacgtagcct acatcccggg cggcaaagtg ctcggactct cgaagctggc gcgcatcgtg
      901 gacgtctact cgcatcggcc gcaggttcag gagcgcctga cgtcgcaggt ggccgacctg
      961 ctcgcaaaac ggctccaggt gaaaggctgt gcggtcgtgt tggaggccgt acacacctgc
     1021 atgacctgcc gcggcgtcaa gaaaagcggg agcgtgatga tcacctccgc cttgcgcggc
     1081 atcatgcaga ccagccaaag cacccgcgcc gaggcgttgt ccctgttaaa ccgctgatca
     1141 ccgccttaat cgcgaaatct catcccgccg cggtgccggc tgttttcgtc gtctcgccgc
     1201 cccggtggcc ggccctcaaa taacgatccg acagcgggct gagcacaggc gcgactgggc
     1261 cggcgtcggc cccctgttcc aggggagacg ggacggataa gattacggtt ttccctgcag
     1321 cttgggcgca ggggcagacc cacaaaaagg agcgcaggat gccggttcca agcgatcgca
     1381 agtattcgca gacgcatgag tggttcaaag ccgacggaaa aatcgtgacc atgggcatca
     1441 gcaagtttgc ggccgacgag ctgacggacg taacctacgt ctctttgccg caggtgggca
     1501 gtcagatcga aggcggcacg cacttcggcg aggtggaatc cgtcaaggcg acgagtgagc
     1561 tgttcacggc cgtttccggc acggtgcgcg aggtcaatcc gcggttggcg gacgagccgg
     1621 agctggtgaa caacgacccg ttcggggagg gctggatgct caaggtggag tgcacggacc
     1681 tgtcgccgct ggacaagctg ctggatgcgg cggcctacga taagatgatt agtgaagcgt
     1741 gaatggcgtc ctcaagttcg ccaagtcacg acgctgggcg ctggaatcat tgtgacttga
     1801 cgacctggat ttgatgactg atttccccgc ccggagttcc ccgcgatatg tctactgact
     1861 cgaacctgcc cgatttgctc ggtccctcgg atagctttgt cgaccgccac gttgggccgc
     1921 gcgacgcgga aatccgcgaa atgctcggcc ttttgggcta ccgcagcctc gacgaaatga
     1981 ttgatgcgac cataccggcg gcgattcggc tgaagaagcc gcttaacctg ggaccgccgc
     2041 ggggcgagcg cgaggcgctc gaagaactgc gcggcatcgc gcggcagaac cagctcttcc
     2101 gttcgctgat cggcatgggg tactacggca ccatcacgcc gccggtgatt ctacggaacg
     2161 tcctggagaa ccccggctgg tacacgcagt acacgcctta tcaggccgag atttcacagg
     2221 gccgcctcga agccctgatg aatttccaga cgatggtgtg cgacctgacc gccctgccgc
     2281 tggccaacgc ttcgctgctg gacgaggcga ctgcggccgc cgaagcgatg acgatgtgcg
     2341 cccgcgtgag caggggcaaa agcaagtgct tcttcgtcgc gcaggattgc catccccaga
     2401 cgatcgcggt ggtgcagacg cgggcgcggc cgctgggttt cgaggtggtg gtcggggacc
     2461 cgctgaagac ggatttctcg aagcaggcct gctttggggc gctgctgcag taccccacga
     2521 cggacggacg agttgagtgc tacgagaaga cgattgagca agtgcatgcc gctggcgggc
     2581 tggcggtcgt cgcgaccgac ttgctggccc tgacgctgct caagccgccc ggcgagttcg
     2641 gcgccgatat tgcgattggt tcgtcgcagc gtttcggcgt accgatgggc ttcggcggcc
     2701 cgcacgcggc gttcatggcg acgcgcactg agtacacgcg gcaaatgccg ggccgcctga
     2761 tcggcatgtc gaaggacgcc gacggcggga tgggtttccg cctgacgctg caaacacgcg
     2821 agcagcacat ccggcgcgag aaggccacca gcaacatctg cacggcgcag gtgctgctgg
     2881 cgatcatggc cgggatgtat gcgacgtacc acgggccggc gggcctgcgg cggattggga
     2941 gccgcgtttt cgccctgacg cacgcgctgc ggacgggact gcggcaactg gggcatgaga
     3001 tcaacgacgc ccacgtgcgg ttcgacacgc tgcgcgttaa accggtcggc cgaaccgcgg
     3061 gcgacgtgat tcgtgcggcc cgcgagcgtc gcatcaatct gcgggactac ggcgatggca
     3121 cagtgggcgt gtcattggat gagacgactg atgtcgccct ggtgcaggat gtgctggcgg
     3181 cgttcggcgg gcgcggcgat gaggacgtgg ttgcgctggc cggctcgccg gacaagctga
     3241 ttccggccgc gctgcggcga acgagcccgt atctgtcgca ccccgtcttc aatagttacc
     3301 acgccgagca cgagatgctg cgctatatca agcggctgga gtcgcgcgat ctgtcgctgt
     3361 gccagtccat gattccgctc ggctcgtgca ccatgaagct gaacgcggcc gcggagatgc
     3421 tgccgatcac gtggccggag ttcgcgtacc tgcacccgtt cgcgccggtg gaacaggcgg
     3481 cgggctacat tgagctgttc cggcagctcg aaggctggct ggcggagatc accggtttcg
     3541 cggcggtttc actgcagccg aactcggggg cggccggcga atacgcgggc ttgatggtga
     3601 tccgggctta ccaccaagcg cgtggcgaag ggcatcgcaa tgtttgcctg attccggttt
     3661 cggcccatgg gacgaatccg gccagttcgg cgatggcggg ctatcaggtc gtgccggtgg
     3721 cctgcgatga gcacgggaac atcgacgtcg ccgacttgaa gcggcgggcc gaggaaaacc
     3781 gcgacaagct ggcggctctc atgatcacgt atccctcgac gcacggcgtg ttcgaggagg
     3841 ccgtacgcga gttgtgccaa attgtgcacg ccaacggcgg ccaggtctac atggacggcg
     3901 cgaacatgaa cgcccaggtc gggttgtgcc gtccgggcga catcggcgcc gacatctgcc
     3961 acctgaacct gcacaagacg ttcagcatac cgcacggcgg cggcggcccg ggcgtcgggc
     4021 cgatcgcgtg tgcggaacat ctgaagccgt atttgcccgg tcatccggtc gtgaatcctg
     4081 cggtcggaag atgggaatgt gggaaggcgg ggacggcctg ctgctctgat gccggccgcg
     4141 tcggggcggt cgcggcggcg ccgttcggca gcccaagtat tctgcccatt tcctggatgt
     4201 acattcgcat gatgggcgag ccggggctga agaaggcgac gcaggtcgcg attctgaatg
     4261 cgaactacat ggcgcaccgg ctgcgcgagc attacccgat ttactacacc ggcaagaacg
     4321 ggcgcgtggc gcacgagttc attctcgact gccgcgaact gaagaaggcg cacgtcgagg
     4381 tcgaggacat cgccaagcgg ctgatggact ttggtttcca cgcgccgacg atgtcctggc
     4441 cggtgcacga cacgctcatg atcgaaccga ctgagagcga gtcgaaacag gaatgcgacc
     4501 ggctggtgga ggcgttaatc gcgatcaaag ccgaggccca ggcgatcgcc gaaggccgga
     4561 tggatgcgga aaacaatccg ctgaagaacg cgccacacac ggcccgggcg gtcacggcga
     4621 cggaatggaa ccatccgtat tcgcgtgagc aggcggcctt cccgaccgag tggctgcgcc
     4681 actacaagtt ctggccgccg gtggcgcgga ttgataacgt ccacggcgac cggcacttgt
     4741 gctgcgcgtg cccggcggat tggggagcgt aggcacagag ccaggttttc cgagcccgaa
     4801 gcgccaacga gggcgaagaa gccgtcagag cccgaagcgc cagcaagggc aagtagccct
     4861 cggagcctga agcgctagcg agggcaagcc ctggcgcgcg ctcgtgctat tgaaggagac
     4921 cggtccgaca gcggggtcag cgaaacggac ttgcggattg cctccaggaa gtcgaggacc
     4981 ggcttgtccc gttgctgctg agatcgtgaa tcaaatcacg gggcggttcg cggggcttgc
     5041 gggaccgccg cggtttcagg acgatagcgg ctggagttct gatactttgg ccaaagggag
     5101 accgctatga aatacggtgc gcggaatcag ttgcgcggcg aggtggtcga gatcaagaag
     5161 gggacggtca tgtgcgaggt caaggtgcgc gtgaccgctc cggcgcagat ggcttcggtg
     5221 atgaccctgg aatcgcttga ggaactcggg ctgaagcagg gcgaccaggt gaatgtcgtc
     5281 gtcaaagccg tcaacgtgct gctgacgaag gactaagcct ggattagagc cgctgctggg
     5341 gattgtgatc gtttgatgcc gcacaagaaa gggcaacccg ccgggccgcg tgcgagcggt
     5401 cgatcggccg tggcgagccc atcgagtacg gacgttcgcg cggaggccgg cggcgtcaag
     5461 cggagcgtgg gggctggccc aaagcggcgt cccccagcac gcggtcagca gaccttcgca
     5521 ctcccggcgg gcgggtggag cgagcggcgg ttctggctga ttctggggtt gatctgcctg
     5581 gtcgcgctca tctgccggct ggcgatcctg gtggagtaca cggcccggaa cccgctggca
     5641 gcgtcgccga tcaatgacgc ccagacatac tgggattggg cggagcgcat tgcgcagggc
     5701 aagctgatac aggatacgcc gttcttttcg gcaccgctgt atccgtactt gctggggctg
     5761 attcgggctg cgggcggttc gctgcggctg atttacctgt tgcagatact ggcagacctg
     5821 gccacggcgt gcctgctggc ttatgcggcg cgagggcgct ttggggcgcg cgtgggacta
     5881 ctggcggcgg cgttgtttct gctgctggag gagccggcct cgttttcgtt acgcattctg
     5941 acgtgttcgt tgcagttgct gctgctggcg atcacgtatt tgctgatgct tcgcgcgcag
     6001 gatgcgatga ccgctcgccg ggccggcgct tcgcaggcag tgacgatccc ggagggaacg
     6061 ctttcagaca gcgggacagc cgtcagttgg attcccgcgc gattcgcgcc ggctctgctg
     6121 ggggtcgcgc tgggcttttt gtgcctttcg tatgcgcccg ccattttact ggtggtggca
     6181 gtcgtgccct ggctgtactg gcagtccggc agaacgcggc gtgatgtgct gccggcgctt
     6241 acgcccttgg ctgtcgcaac cctgctgatt gcgccggcca cgctgcacaa ctggtatacc
     6301 agcggtgact tctttctgat tcaatccgtc acggccgtca acttgcggca ggggaatcag
     6361 ccgcaatcga ttggcgtata cacgcccata ccggatacga cgatcgggcg cgagcgcctg
     6421 ttcgatgatg tggcccggca gtatgagcta gtgcatggcc ggcgcggcac gtgggcggag
     6481 attgaccgct attaccgcga cttggtttgg aaattctggg cggacgatcc cgggcgggcg
     6541 gtgaagctgg cggccaggaa gttctattac ttccttactg cgcgggacta cggcgacatc
     6601 tatcagccgc aggccgagct gcgggccggc atggataggc tgttctggct gacgccgctg
     6661 ccgttggcgt ggctgatcgg gccggcgctg gtcggctttg tgctgctgct gcggcggccg
     6721 attcgattcg cgcccgaatt gctcatgttt cttatcccgc tcatcgtcgt aaccgtgcac
     6781 tggtacacgc cgcgctatcg gctgccggcg atgctggccg tcgttgttgt ggcggcatgg
     6841 gtgtttgaca cggccctgcg ctggcgcgtg cagggcttgc gggcggtcgg cctgggtgcc
     6901 gttgcggcgg ccgcgctggg cgcggaaatg ttgctggggc caatcaaccg ggccgcgggc
     6961 atcgacgtgt gcgatccggc gggcgtgatg ttccatgtcg gaggtgtgtt gggtcagcag
     7021 ggcaagccgg aagacgcggc cgctttgttc cgccggggcc tgcagattcg tcccaatgac
     7081 ccgttggcgc gtgtcgatct gggcgacgtg ttgctcgacc ttggccgccg tgacgaggcg
     7141 ctagctgaat tcgagcacgc ccggcaattc gcgccggatc acccggagtt gctcgggcgg
     7201 attgccatgc tattgatgga acggcagcgc ttgccagaag cgcgggccgc cctggagcgc
     7261 gccctgccgc gcgacccgcg taacccggtg ctgctgcggc tcgcggccga ttgttaccag
     7321 cgcatcctgc gggccgatcc gaacgacgcc gcggcgcgca acagtttgaa ttgggttgag
     7381 cagcagttcc ggacagggcg gccgggcgga cggccggaag cgattcgcgc gtcggagcgg
     7441 ccgtaagcga tccaagcaac gggccggcgg agccgcgggg cgagcttggg ccgcaggcgg
     7501 tgcgtggttc ggttatacta gtattcgccg tgaggagagg tgcccgagcg gctgaagggg
     7561 ccggtcttga aaaccggtga accgcaaggt tccatgggtt cgaatcccat cctctccgtt
     7621 ctgcagatct cgcgccctta cgtgcgcccc gcgatccgat aattgttgcg actccggctg
     7681 cgaatagggc ggctcgtagc gcatctgaat aaagccctcc ccaatgacga taacttctgc
     7741 gcgcccgctg gaccggctac ggagcggccg gccagcgcag gaactgcgaa agaggatggt
     7801 ggccggctcg gctgggttgc gcgccgcgcc cgccgagccg cggccccgcc ggttcaaaac
     7861 tgcgacggcc tgaagactgc accggttgaa gcctgcaacc ggttaaggat cgcgagcggt
     7921 tgaaaacggc accggtcgaa aaccgctccc aaaaggatta actccgcgag gattgttatg
     7981 cgcatctccc gaacaatgtt gggcctgttc atcgcgacga ttctctttgt actcacgctc
     8041 gcggcgcgcg gcgctgctca gcccgcctac cgcgcgattg aattgggcac gaccgggggc
     8101 gatcaaagcg aggcctacgc gatcaacgac tacgccgaga tcgtcgggcg gtctttcgac
     8161 gccaccggcg aggcccaggt gttctactgg caaaatggcc agatactggc gttgtttcac
     8221 gagatgccct gggggcgcat tccgatgccc gccacgaggt actcggtcgg ctacggcatg
     8281 tccaacaccg gctacgtcat tacgtacgtt atggacgagt cggaggacga cccgggtctg
     8341 ctggtcccga gcgcggtcat ctttcgccca acgctcagcg acttgaccac cccctaccag
     8401 gcccaggcaa tcacgtacat cggcacgctc tgcagcccga ccggcatcag ctcgtcgggg
     8461 cgctacgtag tcggctgggg gcaattggac tgctcgagcc gggccgaggc attcctggta
     8521 acgccgatga atggcacgtg ggcggtgccc gacgggacgt gcgtcaacaa tgcgctggtg
     8581 attggcctcg gaacgctgga agctttggac atggtcagca ccgcgaccgc cgtcaacaac
     8641 cacggccaag tggtcggctg gtcctatagc gacaccggcg gctatcaggc gttcctgata
     8701 aaccccaccg tcgacgtcga cggcaacccg gtcgcctggt tcacggatga cggcgaaggc
     8761 aacaacgcac tcatgacgcc tctcggaacg ctgccgggca ccggcgggga atcgttcggc
     8821 acaaacagtt tggcacgcga catcaacgac gccggcctga ttgtcggtga ggcggacacc
     8881 ggcgattacg tgacgcacgc cttcctgtac gcaaacggcg ccatgctcga tctgggcacg
     8941 ctgggcggac cggccagcag cgcctgtgcg atcaacgaga acggtcagat cgtcggctgg
     9001 gccctcaacg cggccggctt accgcgggca ttcgtcatca ttccggcgga tgccgacgac
     9061 gacggcgcgc ctgacacgtg gtatgcggac gaagacgagg acggcataaa cgacctgatg
     9121 atcgacctta accacgtggt caacagcggt ttcgcgctgc aactcactga agcacgcgac
     9181 gtcaacatcc acggccaaat cgttggttgg ggaaccagcg gaacgggcga cctggcgctg
     9241 cgcctggcgt tcctgctgac gccggccgcg cctgcgaacg gcaataatga caacggggac
     9301 agtggaccgg tgctgggcga cgtgaatctg gccccgctgc tgagcgatcc ggccgagcag
     9361 cccgagcagc ccggcgaaaa cccccaggcg cctgccccaa cgccgagcct cggactcgcc
     9421 cattggctgt gcggcgtggg catggtgcaa ctgttgccac tgatgctggc gggcttgtgg
     9481 gcgatgaagt tgagcttccg gcgccgctag gacagttcga atcggaatga accaggcgag
     9541 cccgatccgc cggcggatcg ggctcgttgt tttattactg ggcggcggca tgcccccctt
     9601 ttctacgcct ccccgcctga aaacccgggc cgcgtgaaaa gcgcctggcg gcgcgataca
     9661 gtactctgca tgtttgacgc tctttccgag aaattaaccg gagtcttgcg cagtctgcgc
     9721 ggacgcgggc gcatcaccga ggacaacgtg cgcgaggcca tgcgcgaggt gcgcacggcc
     9781 ctgctcgagg ccgacgtgca catcgaggtg gcccgcaagt tctgcgacga gtgccagcag
     9841 aaggccctcg gcatgcaggt cctcgagacc ctgcggcccg agcaggtcat gatcaaggtt
     9901 gtgcacgatg agctggtccg cctcatgggc cccatcgaaa cccgcatccc cttcgtctca
     9961 cagccgccga ccatcatcct catggccggc ttgcagggct ccggcaaaac caccacctgc
    10021 gccaagctcg cccgctactg cttgcagaag ggcaaaaagc ccatgatggt cgcggccgac
    10081 ctgaagcggc ccgcggctat cgatcagctt gaagtgctgg gcacgcaggt cggagtaccg
    10141 gtctacgtcg aacgcgacca ccagaacgcc gtgaaagtct gccgcaacg
//
LOCUS       DAOSYS010000131        10174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5610_length_10174_cov_5.525941, whole genome shotgun sequence.
ACCESSION   DAOSYS010000131 DAOSYS010000000
VERSION     DAOSYS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_10174_cov_5.525941"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1202
                     /locus_tag="PLU99_09320"
     CDS             345..1202
                     /locus_tag="PLU99_09320"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOO17297.1"
                     /translation="MSIGEYIAPAWRERLAAAGLARPADLLDHSPLELGLAGNWECLA
                     KPGLGRRERWRWELPNGAGSELLYLKRYGPADWKSTWDRMLRQSARHGRAWWECDQSR
                     LLSAAHVSAPQAIAAAEHMRGISERGSIVLLSAAEGDAFDRVWARAVEANEAVVHGRA
                     RHNVAIALARFIAAFHATGLCHRDLYLCHVFTKLDLGGRAAPQFTLIDLARTFRPRWR
                     RLRWIIKDLSQLDASAQQIGARRTDRLRFLHAYLGPSLKASIRSYARRVVRKSDWILA
                     RIARKSRST"
     gene            1199..2416
                     /locus_tag="PLU99_09325"
     CDS             1199..2416
                     /locus_tag="PLU99_09325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17298.1"
                     /translation="MKVALIQETIEAARGGAETSTRQMASHLAALGLEVTVVCAAGAR
                     QGALSAPFEEDEVSYRPLPARGVSRTLRSQRFLRAAAGFCRTSQFDIVHAITPCAVAD
                     VYQPRGGTLPETVRRSTAMVRRPLLRRLRHLGKLMNMRQRWLCAVERRLLAPGSEVHV
                     AALSEYVRRQVLTDYSVVPQRVHLIFNGVEVAALEEPERAAHRAAVRTQLGIPEHAAL
                     VLFVAHNFRLKGLRELIQAGALDREDGPRWLLAVVGRDAPGQYLRLASRLGIADRVRF
                     LGSQTDLRPWYAAADVLAHPTWYDPCSRVVLEALSLGLPVVTTRWNGAAEVLEPGKHG
                     AVIETPADTAALAAAIAEALDPAVRCACRADAPRLRALVSMERHARQLLALYEEILAA
                     RGAGSAGWRAATS"
     gene            complement(2406..4586)
                     /locus_tag="PLU99_09330"
     CDS             complement(2406..4586)
                     /locus_tag="PLU99_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HOO17299.1"
                     /translation="MSNTGAPGNEFGVLARTSRPGPQPFVLKAGETFGVFDSFGDLRP
                     DESSEQGVYHAGMRHLSRLEFQIWGAGPLLLSSTVRQDNCALTVHLTNPDITRADQVI
                     VPRGALYMARTTVLAEHACVQRMRVSSYSDQEIVIPVRLLAAADFCDIFQIRGMPRTQ
                     YGRPRPPTFEAGELTFEYEGRDQVVRSTRLRVRPAPDRHFVGGVELQYTLPPRATSEL
                     EFIFDFRGELPAPKLQVSFERARDLQCDSLARARQSACRITTSHEQFNDWLERSFVDV
                     HMLVTTTQFGRFPYAGIPWYSAPFGRDGLITALELLWVDPSLARGVLSFLANTQADHT
                     DPQCEAQPGKILHEARLGEMAATGEIPFQRYYGSVDAPALFVFLAGEYFRRTGDREFI
                     ESIWSNIVRALDWLDNFGASKIDGFIDYRRIAGRGLVNQGWKDSTDSVFHADGSDPPP
                     PIALVEVQAYTYAARLGAARLARELGDLTRAATLAQQAETFRQRFVRDYWCPDLNTYV
                     MALDGNRRPCRVRTSNAGQTLFCGIATTEQARAIMQTLLSPGSFSGWGIRTVDTTAAR
                     FNPMSYHNGSVWPHDNALIALGFARYGFKRAVLEILSGMFESCLFMTMHRMPELFCGF
                     PRVPEEGPTLYPVACLPQAWASAAVFLLLQACLGLNFRLDETPGRVTIELDRPVLPDF
                     LRRIEIDDLRIGEEWVNLVLQNHEGDVGVNIRQRSANLDVIVKK"
     gene            complement(4579..5643)
                     /locus_tag="PLU99_09335"
     CDS             complement(4579..5643)
                     /locus_tag="PLU99_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17300.1"
                     /translation="MRIAQVAPLFESVPPRQYGGTERVVSFLTEELVSRGHDLTLYAS
                     GDSQTRAELVAIVPHALRPSGQEESATAYVALQLERVLGDAGRFDIIHFHTDHAHFPI
                     TRSLKAAHVTTLHGRLDLPELQALYREFRDVPVVSISDSQRRALPLARWIATVHHGLP
                     LDKYAFHERPGDCLVFVGRISPEKRLDRAIEIAKRSGLRLLVAAKIAVKDREYYEREI
                     RPLMDHPLVTFIGEIDDDQKCELMGQALALVFPIDWPEPFGLAMLESMACGTPVIAWP
                     CGSVPEVVEDGVTGYLVDSVEAAVEAVRRVPQLDRRNVRRRVEARFNAARMADDYLRV
                     YRLLIAAARPPATLAQGAYV"
     gene            complement(5893..6324)
                     /locus_tag="PLU99_09340"
     CDS             complement(5893..6324)
                     /locus_tag="PLU99_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17301.1"
                     /translation="MLRTGMHSKKCLLLLALPLLAFVAGCPLPATAVLEGTWAFTVDS
                     TPNLNELLLTFNQNGQVTNVTYQVTGSAEISVNAPVGQATVNGKNVTVTSTFQGNSLT
                     FNGTLNDDNTVAQGTITLQITVGAVTVTVNGGAATMTKLLQ"
     gene            complement(7081..7260)
                     /locus_tag="PLU99_09345"
     CDS             complement(7081..7260)
                     /locus_tag="PLU99_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17302.1"
                     /translation="MRKRISMMLLASLTTVIASGCCASLGRNIWVGFGESIGAVPASF
                     LVNFLANTFGIDVQA"
     gene            complement(7434..7613)
                     /locus_tag="PLU99_09350"
     CDS             complement(7434..7613)
                     /locus_tag="PLU99_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17303.1"
                     /translation="MRKRMIALMLVASAATLFNGCFGSLQRNIWVGFGESIGAVPAGF
                     LVNFLANTFGIDVQA"
     gene            complement(7951..>10174)
                     /locus_tag="PLU99_09355"
     CDS             complement(7951..>10174)
                     /locus_tag="PLU99_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17304.1"
                     /translation="VLLALSLAGVLELWVFRGARAWMSVAPGELLPLGRTVVAALAFV
                     SPVSALVGLAFPLACAVTLSVAPATQADPRSADGHRPALSFAHVYAIESAGSLIGGAA
                     FSFWAVERLAPIQIVLLCGAATAVCSAGFLLVHPGRAAGPSRRWRRLPPTAAALAGLS
                     AIAIMAALFGGEPIDRWLTARRWSQLAPGYELVASSETKYQNLALGRRAGQFSLFCDG
                     HFASDFPDPYTWAPLAHFWMCQHPQPRRVLVLGGGTEGLLSEILLHPVEQVDCIYPDP
                     RELELVQPFLPAADRDALADPRVTIHATDARHFVKTQRALFDLVVARPPEPTSALRAR
                     FYTDEFYAELRRAMTPEAVLCLTAAAAPTQLSELSRQYLGSIRETLRRHFPELIIGWG
                     DPAHVLAATVPGLITIDPAEMSRRYARRSVKSPTFDPAWFAGATDWLDLEKVAQRAAD
                     LDAAEGVTASTDLKPSIYLQRLALWEAAAGAGAARRDFVDTASNSWLSKWCAAGVFAQ
                     LKSLRLTHLISLLAGAVALTLLICRLRQGPRTGWAAGAIAVSVATTGLATMALSILWL
                     FAFQNLYGYVYQRIGWIIALFMAGLVLGCMLFGHGRLRLRARLIRLDVLLAVLAASIP
                     FVWQALARLQSGPGSFGVVEGCISIMVGMTGLLGGAAFGVAGRWQFEMTGAAGAAAAR
                     VVSADHVGACLGALLCGIVLVPVWGIAITAFLLVGIKVLSALLLWFLGRSYVDAAFQI
                     "
BASE COUNT         1936 a         3153 c         3340 g         1745 t
ORIGIN      
        1 gagttctttt ttctcgatgc ggctccgcga cgctggcgaa aagtggcgtc cagtcagccc
       61 tggtagcagc gtgagctgtc gcacctgacg gtttccgggc tgccgttcac tacgcggtct
      121 gaccggctgc gtttcctgct gcgatatttc agagccacgc ggctgaacgc tgagctcaag
      181 cggcaggcgc gcgagatcgc ggccggcgcg acgcagtggc agcggcacga atcgcagcgc
      241 atcaagatga accggctgtt cgacaagtgg aacgagcggc taatgcgaga agagcgcctg
      301 cagagttcgc cggccgtgag cccggccggg ccaggggacc tggtttgagc atcggggaat
      361 acatcgcccc ggcctggcgc gagcggctgg ccgcggccgg cctggcccgc ccggccgacc
      421 tgctcgatca ttcaccgctg gaactgggcc tggctggcaa ctgggaatgc ctggcgaaac
      481 cgggcctggg gcggcgcgaa cgctggcgct gggagttgcc gaacggcgcc ggttcggaat
      541 tgttgtattt gaagcgttac ggcccggcgg actggaagag cacctgggac cgaatgctgc
      601 ggcagtcggc tcgtcacggg cgagcgtggt gggaatgcga tcagagccgg cttttgtcgg
      661 cggcgcacgt ttctgcgccg caggccatcg cggcggcgga acacatgcgc ggaatttcgg
      721 agcgcggcag catcgtgttg ctctcggccg ccgagggcga cgcgttcgac cgcgtctggg
      781 cgcgggcggt tgaggcgaat gaggcggtgg tgcatgggcg cgcgcgtcat aatgtcgcca
      841 tagctctcgc ccgtttcatt gcggcgtttc acgccacggg tctgtgtcac cgcgacctgt
      901 acttgtgcca tgtattcacg aagctggatt tgggcggccg ggcggcgccg cagttcactc
      961 taatcgacct ggcccgcacg ttcaggccgc gttggcgtcg gttgcgctgg attatcaagg
     1021 atttgtcgca actggacgcg tcggcgcagc agattggggc gcggcgcact gatcgcctgc
     1081 gcttcctgca cgcgtatctg ggtccgtcgc taaaggcttc tattcgctcg tatgcgcgcc
     1141 gcgtggtgcg caagagcgac tggattttgg cgcgcatagc gcgcaagagc cgcagcacat
     1201 gaaagtcgcg ctgatccagg agaccatcga ggccgcccgc ggcggggccg aaacctcgac
     1261 ccggcagatg gccagtcacc tggcggcgct gggtctggag gtgactgtgg tctgcgcggc
     1321 cggtgcgcgt cagggcgcct tgagcgcgcc gttcgaagaa gatgaagttt cttaccggcc
     1381 cttaccggcg cggggcgtga gccgcacgct acgctcgcag cgtttcctcc gcgcggcggc
     1441 ggggttctgc cggacatcgc agtttgacat agttcacgcg attacgccgt gcgccgtggc
     1501 cgacgtctat cagccgcgcg gcggcacact gcccgagacg gtgaggcgca gcacggcgat
     1561 ggtgcggcgg ccgttgctgc gccggctgcg gcatttaggc aaactgatga acatgcgcca
     1621 gcgctggctg tgcgcggtcg aacgccgatt gctggcgccg ggctccgaag tgcatgtagc
     1681 ggcgctgtct gaatatgtgc gccgccaagt cctcactgat tacagtgtcg tgccgcagcg
     1741 cgtacacctg atcttcaacg gcgtcgaggt ggcggcgctg gaagaacccg agcgcgcggc
     1801 gcaccgcgcg gcagtccgca cgcaactggg gataccagag cacgccgcac tggtattgtt
     1861 cgtggcgcac aacttccgcc tgaagggact gcgcgagttg attcaggccg gtgctctcga
     1921 ccgcgaggac gggcctaggt ggctactggc ggtagtcggg cgcgatgcgc ccgggcagta
     1981 tctgcgtctg gccagccggc tgggcatcgc ggaccgcgtg cgtttcctcg gatcgcagac
     2041 ggatttgcgt ccgtggtacg cggcggcgga cgtgctggcg catccgacgt ggtacgatcc
     2101 gtgcagccgt gtcgtgctgg aagccctaag cctggggttg ccggtggtca ccacgcgctg
     2161 gaacggggcg gcggaagtgc ttgaaccggg caagcacggc gcggtcatcg agacgccggc
     2221 ggatacggca gcgctggcgg ccgcgattgc ggaagcgctt gatccggccg tgcgttgcgc
     2281 ttgccgagct gatgctccgc ggctgcgcgc cctggtttcg atggaacggc acgcgcgcca
     2341 gttgttggca ctctatgaag agattctggc ggcacgcggc gcgggcagcg cggggtggcg
     2401 cgcggctact tcttgacaat cacgtccaga ttagccgagc gttgccggat attcacaccc
     2461 acatcgccct cgtggttttg cagcactaag ttcacccatt cctcgccgat gcgcaggtcg
     2521 tcaatctcta tgcgccggag aaaatccggc aacaccggcc ggtccagttc gatcgtcacg
     2581 cggcccgggg tttcgtcgag ccggaagttc agccccaggc acgcttgcag cagtagaaag
     2641 accgcggcgc tggcccacgc ctgcggcagg caggccaccg gatatagtgt gggaccctcc
     2701 tccgggacac gcgggaagcc gcagaacagc tccggcatgc gatgcatcgt catgaacagg
     2761 cacgattcaa acatgccgga caagatttcc agcaccgccc gcttgaagcc atagcgcgcg
     2821 aacccgaggg cgatcagggc attgtcatgc ggccagacgg agccgttgtg ataggacatg
     2881 ggattgaagc gggccgcggt ggtatcaacc gtacggattc cccagccgga aaaggaaccc
     2941 ggggacagca gtgtttgcat gatggcgcgg gcctgctcgg tggtggcgat gccgcagaat
     3001 agcgtctgcc cggcattaga ggtgcgaacg cggcacggcc ggcggttgcc gtccagagcc
     3061 atgacataag tattcaagtc cggacaccag tagtcacgca cgaaccgctg gcgaaatgtt
     3121 tcggcctgct gtgcgagcgt ggcggcccgg gtcaggtcgc ccagttctcg cgccaggcgg
     3181 gcagcgccca ggcgcgccgc gtaggtatag gcctgcactt cgacgagtgc aatgggcggg
     3241 ggcgggtccg agccgtcggc gtggaagacg gaatcggtcg agtctttcca gccctggttc
     3301 acgagcccgc ggccggcgat ccggcggtag tcgatgaagc cgtctatttt ggaggcgccg
     3361 aagttgtcga gccagtccaa tgcgcggact atgttggacc agatggactc tatgaactcg
     3421 cggtcgccgg tgcggcggaa gtactcaccg gccagaaaga cgaagagcgc cggggcgtct
     3481 acggagccgt agtagcgttg aaaggggatt tcgccggtgg cggccatttc gcccaggcgg
     3541 gcttcgtgca ggatcttgcc gggttgggcc tcgcactgcg ggtcggtatg gtccgcctgg
     3601 gtgttggcca agaagctcaa cacgccgcgg gccagcgacg gatcgaccca gagcagctcg
     3661 agggccgtga tcagcccatc gcgtccgaag ggagcgctgt accagggaat gccggcgtag
     3721 ggaaagcggc cgaattgagt agtggtgacg agcatgtgta catcgacgaa ggagcgttcg
     3781 agccagtcgt tgaactgctc gtgtgaggta gtaattcggc aggccgattg gcgggcgcgg
     3841 gccagcgagt cgcattgcag gtcgcgggcg cgctcgaacg acacctgaag tttgggggcc
     3901 ggcagctcac cccggaaatc gaaaatgaac tccaattcgc tggtggcccg cggcggcaat
     3961 gtgtattgca gttccacgcc gccgacgaag tgccggtccg gcgcgggccg gacgcggagc
     4021 ctggtgctgc gcacgacctg atcgcgcccc tcgtactcaa aggtcagttc gcccgcttca
     4081 aaggtgggcg gtcgcggccg cccgtactgc gtgcgcggca taccgcgaat ttggaagatg
     4141 tcgcagaaat cggccgcggc cagaagtcgg acggggatga cgatctcctg gtcggaatag
     4201 ctgctcaccc gcatgcgctg tacgcaggcg tgctcggcca ggacggtggt tcgcgccatg
     4261 taaagcgcgc cgcgcggcac gatgacctga tcggcgcggg tgatgtcggg attggtcagg
     4321 tgaacggtaa gcgcgcagtt gtcctgacgc acggttgagc taaggagcaa tgggccggcg
     4381 ccccagatct ggaattcgag gcgcgagagg tgccgcatgc ccgcgtggta caccccttgt
     4441 tcgctgcttt cgtcggggcg caaatcgccg aatgagtcga atactccgaa cgtctcaccc
     4501 gctttgagca cgaacggctg cggtcccggg cgcgaagtcc gggccaggac cccgaactcg
     4561 ttgcccggcg caccggtatt agacatacgc accttgagca agggtggccg gcgggcgtgc
     4621 cgctgctatc agcaaccggt acacgcgcaa atagtcatcc gccatgcggg ccgcgttgaa
     4681 gcgggcttcg acccggcggc gcacgttgcg gcggtcgagt tgcgggacgc ggcgcacggc
     4741 ctccaccgcc gcctcgacgc tgtcgaccag gtagcccgtt actccgtcct ctacgacctc
     4801 aggcacggag ccgcagggcc aagcgataac cggcgtaccg cacgccatgg actcgagcat
     4861 ggccaggccg aacggctcag gccagtcaat cgggaacacc agtgccaggg cttggcccat
     4921 cagttcgcat ttttgatcgt catcgatctc gccaatgaac gtgacgagcg ggtgatccat
     4981 cagaggcctg atttcgcgtt catagtactc gcggtctttc acggctattt tcgcggccac
     5041 caagagcctc aacccgctgc gtttggcaat ttcgatcgcg cggtccaagc gtttctcggg
     5101 tgaaatgcgg cccacgaaca cgaggcaatc gccggggcgc tcatgaaacg cgtatttgtc
     5161 gagcggcagg ccgtggtgga cggtggcaat ccacctagcc agcggcaagg cgcggcgttg
     5221 cgaatcggaa attgaaacca ccggaacgtc gcggaattcg cgataaaggg cttgcaattc
     5281 cggcaggtcc agccgcccgt gcagcgtcgt aacatgagcc gccttcaggc tgcgtgtgat
     5341 gggaaaatgt gcgtgatcag tgtgaaaatg aatgatgtca aatcggccgg cgtcaccgag
     5401 aacccgctcg agctgcaggg cgacgtatgc ggttgcggat tcctcctggc cgctggggcg
     5461 caatgcgtgc ggcacaatgg caaccagttc cgcccgcgtt tgagaatcgc cgctggcgta
     5521 aagggtcagg tcatgcccgc ggctaactaa ctcttcagtc aggaaagata cgaccctttc
     5581 agttccgcca tactgcctgg ggggcacgct ctcgaacagc ggcgcaacct gtgcgatgcg
     5641 cataacaaat ccttcgcgct gggccggcat caaatgctcg catgcggaaa gccgacccag
     5701 gtcgcgggat tctattcacg ccttcttcgg cgttccgggc gaaaggcccg gcgcctcgac
     5761 gaggccggcc gacgatcaga ctaccagaca ggtctggact gaaacccgtc cgcgccatcg
     5821 tttgagcgcc gttgaagcgc gtcggatggt tgtcttctgc gccgcgttag cgactgggtc
     5881 cgccgcagca ggtcactgca gcagtttcgt cattgtggcc gcgccgccgt tgacagtgac
     5941 cgttacggcg cccacggtga tttgcagtgt gattgttcct tgagcgacgg tattgtcgtc
     6001 gttgagcgtt ccgttgaagg tcaacgagtt gccttgaaag gtcgaggtca cggtgacgtt
     6061 cttaccgttg actgtcgcct ggccgacggg ggcattgact gaaatctcag cgctgccggt
     6121 aacctggtat gtgacgttag taacttggcc gttttggttg aatgtaagca gcagctcgtt
     6181 aaggttgggg gttgagtcga cggtaaacgc ccaggttccc tcaagcaccg ccgtggctgg
     6241 aagggggcag cccgctacga aagccagcaa gggcaacgcc aacagcagca ggcacttctt
     6301 ggaatgcata ccagtccgaa gcatttactt ctccttcagc tacggtgaat gtctgacccg
     6361 ggggtcgcag ctactcacac aaccccgacc gcggctttct catgcctgat gaagccggct
     6421 cgggtagagc gggcgcgcac agcggttgag tataggcgcg agcgatcccg acgagtctag
     6481 cattgcgacc agacaccgcg ggctggatga actggcacgg tgtaggattt tcggcactgc
     6541 cagcgtcgct atttagcgct cgcaaacgcg acgtgccatg tcgtatgaac gagcttcagg
     6601 gaatggcagg cgcggaagga ggcggcttac gaaattcgca cttccaggag agtgacgttc
     6661 tcgccgaacg gatcaggtgc aaggccgcaa gcaatgcgta ctctgccgcg ggactactcc
     6721 gtgaattcct ctgcggcctt gctctaatag accatgcagg gcgtggtgtc agtatgtgaa
     6781 tggctacgtt gacggaattc acgaccagca cggtcgtgat ttccacaaag atgtctcgcg
     6841 ccacggcctc ccgacggcca ccaccggatt ttggtactct ccccgcgtgt tctagcagcc
     6901 gcgagaactg atcggcccgg catcacactg acgcgagtcc gacagtccgg aaaaaaaccg
     6961 tgcccgcgcg gggcacgggc acaattcagc ttcgaactgc gttacgacct tgcgcgggca
     7021 tcgtttgctg tgcccagaca acgccgggca acgccacacc tgcgcaagcg tcacaacgtc
     7081 ttacgcctgt acgtcaatcc caaaagtgtt ggccaggaag ttgaccagga aactggccgg
     7141 gacggcgccg atcgactcac caaagccgac ccagatgttg cgaccgagac tggcgcagca
     7201 accgctggcg atgaccgtcg tcagcgaggc caacagcatc atactaattc gctttcgcac
     7261 agaagcctcc ttcaagacct gatcttgcat ccatgccgtc tgtcagacgg cacgcaaagc
     7321 ctgctttgcg ccttgcgtta aggctaaaaa cgtgacgacc tgcgccaggc ctgaactgtc
     7381 gcgccggaaa caaagccggg caacgccaca ccctgcgcag gcgtcacaac gttttacgcc
     7441 tgtacgtcaa tcccgaaagt gttcgccagg aagttgacca ggaacccggc cgggacggcg
     7501 ccgatcgact caccaaagcc gacccaaatg ttgcgctgca gactaccgaa gcagccgttg
     7561 aacagggtgg cggcggacgc caccaacatc aaagcaatca tccgctttcg cacagaagtc
     7621 tccttgaaga tctgaccttg cttccactcc accgtccaaa ccgcatgaac ggtgtacgct
     7681 tgttgtgcgg ttactcgcag gcgcgataat cgctacaact gtcgcgcgcc tgacgaaact
     7741 ttcccaagta tacgcgatgg acacgggctg tcaacaggcc atccgcagag aaaggacgat
     7801 cagtacggga agtgtccaca atttgattgg cgaccgacgc tgtctatgaa ccgggcacct
     7861 acggtgcagc aaaaccttgg cataactgcc caaccagggt ggcggaaaat cgccacctaa
     7921 agactccggc tgtcatgcct cgccacgttc tcagatttgg aaagccgcgt ccacatagga
     7981 gcgcccaaga aaccacagca gcagcgctga aagcactttg atgcctacca gcaagaacgc
     8041 cgtgattgcg atgccccaga ccggtaccag cactatgccg caaagcagcg ccccgaggca
     8101 cgcgcccacg tggtcggcac tgaccactct tgccgcggcc gcgcccgccg cgccggtcat
     8161 ctcgaactgc caacgaccag ccacgccaaa cgcggccccg cctaacagcc ccgtcatacc
     8221 taccattatg gatatgcagc cttcgaccac cccaaaactg cctggaccac tttgcagtct
     8281 cgccaacgct tgccagacaa agggaatcga ggcggcaagg acagccaaga gaacgtctaa
     8341 ccggatcagc cgcgctctca agcgcaatcg gccatggccg aatagcatgc aacccagcac
     8401 cagtccggcc atgaacagtg cgattatcca tccgatgcgt tggtacacgt agccgtacag
     8461 gttttgaaac gcgaatagcc acaggatgct gagggccatg gtggccaggc cggtagttgc
     8521 caccgatacc gcgatcgccc cggccgccca gccggtgcgc ggcccttgtc gcaatcggca
     8581 gatgaggagc gtgagggcga cggcgccggc caggaggctg atcaggtgcg tcaggcgcag
     8641 cgacttcaat tgtgcgaaca cgccggccgc gcaccacttg ctcagccagg aattggaggc
     8701 ggtgtccacg aagtcacgcc gggcggcgcc tgcgccggcc gcggcttccc acagcgccaa
     8761 gcgttgcagg taaatggagg gcttcagatc agtgcttgct gtaacgcctt cggccgcgtc
     8821 caggtcggcg gcgcgctgtg cgaccttttc gaggtcgagc cagtccgtgg ccccggcgaa
     8881 ccatgccgga tcaaaagtcg gagacttgac gctgcgacgc gcatatcgcc tgctcatctc
     8941 ggccgggtca atggtaatca gccccggcac ggtggcagcc aacacgtggg ccgggtcgcc
     9001 ccaaccgatt atcagttcgg gaaagtgccg ccgcagcgtt tcgcgtatgg agcccaagta
     9061 ttgtcgcgaa agctcggaca actgcgtcgg cgcggccgcg gcggtcagac aaagcaccgc
     9121 ttccggtgtc atcgcgcggc gcagctcggc gtagaactcg tccgtgtaaa aacgggcgcg
     9181 cagcgctgat gtcggctccg gcggccgagc caccacgagg tcaaacaggg cccgctgcgt
     9241 cttgacgaaa tgccgagcgt cggtggcgtg tatcgtcacg cgcgggtccg ccagcgcgtc
     9301 gcggtctgcc gccggcagga acggctgtac gagctcgagt tctcgtgggt ccggataaat
     9361 gcaatccacc tgctcgaccg ggtgcagcag aatctcgctc agcaggcctt ccgtgccgcc
     9421 ccccaatacg agcacgcgcc gcggctgcgg atgctggcac atccagaagt gcgccagcgg
     9481 cgcccaggtg tatggatcgg gaaaatccga cgcgaagtgg ccatcgcaaa atagggaaaa
     9541 ctggccagcg cgccggccaa gcgccaggtt ctgatatttc gtctcgctcg acgcgaccag
     9601 ttcgtagccc ggagcaagtt gactccagcg gcgggcagtc agccagcgat caatcggctc
     9661 gccgccgaat agcgctgcca tgatcgcgat ggccgagagt ccggcgagcg ccgcggccgt
     9721 cggcggcaat cggcgccacc tgcgactggg gcccgcagct cggccggggt gcaccagcag
     9781 gaagccggcc gaacacacgg ccgtggcggc gccacacaac aggacgatct gtattggggc
     9841 caagcgctcc acggcccaaa agctgaaggc cgctccgccg atcaggctgc ccgctgattc
     9901 gatggcatag acatgggcga aggagagggc cggacgatga ccgtcagcgc ttcgagggtc
     9961 ggcctgggtc gccggcgcca cgctcaaagt aaccgcgcag gccaacggaa aggccaaccc
    10021 gaccagggcg ctgaccggcg agacgaacgc cagcgccgca acgaccgtcc gccccagcgg
    10081 cagcagctca cccggcgcca cactcatcca ggcccgcgct ccgcggaata cccacaattc
    10141 aagcactccg gccaggctca gtgccagcaa cacg
//
LOCUS       DAOSYS010000132        10046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5707_length_10046_cov_5.155940, whole genome shotgun sequence.
ACCESSION   DAOSYS010000132 DAOSYS010000000
VERSION     DAOSYS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_10046_cov_5.155940"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..743)
                     /locus_tag="PLU99_09360"
     CDS             complement(342..743)
                     /locus_tag="PLU99_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17305.1"
                     /translation="MDIEHPIGLWELDSGTLLTSGVISQGATDPLINHFRYVDVQDVV
                     LVPGVDYVIGFFTGASNQDRMVYAAPTDLVIDPAITYVQARYGGAPAPAMPGPTTSTT
                     YCRFGPNFQYIPEPAALGLFLAAGLLLRRRG"
     gene            complement(1024..3285)
                     /gene="feoB"
                     /locus_tag="PLU99_09365"
                     /pseudo
     CDS             complement(1024..3285)
                     /gene="feoB"
                     /locus_tag="PLU99_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
     gene            complement(3339..4355)
                     /locus_tag="PLU99_09370"
     CDS             complement(3339..4355)
                     /locus_tag="PLU99_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOO17306.1"
                     /translation="MTAVYLILILLAVAVFWPRHGLLARWRQAHDLARRMRREDALKH
                     ILKSEANGRVPTLESIAGALHITTGSAARLLDELASHDLLTFEGGQLRLRPAGREMGL
                     HVLRAHRLWESYLAEETGIAEDEWHRRAERQEHLLTPAQTRALAARLGHPTRDPHGDV
                     IPSSDGTFEGEDSQSLNSVEPETPVLITHIEDEPETVYSQLVALGLRAGMHAFVIEKT
                     PTRIRFWADGNEHTLAPVLAENVSIRPLPDYGTADLVEEEFLSGLPPGRRARVVGLSG
                     ACRGPERRRLLDLGFVPGTPVEVEMVSPAGDPTAYRVRGSVVALRREQANLVRINSRE
                     AVAT"
     gene            4730..6790
                     /locus_tag="PLU99_09375"
     CDS             4730..6790
                     /locus_tag="PLU99_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO17307.1"
                     /translation="MLFVSTLILVSYPALVATVPPARSLNYVDSSSGLQFPEMYSGPT
                     ELEFADVNGDGHVDIGMVGDHDSPGMSQHGITVWLGDGAGHWSARQSGYFGYGGIAFG
                     DVNGDGLMDVGYAMHHTDPGSDFGDQLIEVALGDGTGENWIPWDDGLATSGETWGMFG
                     TDFADVDNDGDLDIGSISFGCCAGLHVYLNNGDGTWTQSWGVLDGDAYEIFQFGDING
                     DGFIDFAAAHSYGTVWLGDGQGNFTVADYGLPGGVGDWREGVALGDVNGDGRDDLAFR
                     NANGGIDVWSYVGPGVWQNLSGTLPASGNFYYTQIADMNLDGHGDIVACARGQIRVYG
                     GDGAGNWELLTTVNIGGSGAKAFRAGVDADHNGYPDIVMVNREPNNRARFYRETTPVS
                     STWVYPKYPRGGEVMYAGSVRFIDWHAAVANGESTVTIELSVVGPDGPWIEIASNVPN
                     NGRFQWHLPRHLTSSNTCYLRYTLGEAQAVTPAPFTIVGEPGMAPLSISLPEGTPEFL
                     SPAAPTRFTVEIVSGGEAYVPGSALLHYCYDGGEFITRPLTPLGETLYQATLPAPACA
                     DTPQFYISAVGDGGSTVYSPEGAPENVYTARVGEVRALLVDDFETDPSCYDRGDLNCD
                     GALNVFDIDAFVLALTDPLEYATALPYCNCKLGDINCDEAVNVFDIDPFVGCLTGGGC
                     APCP"
     gene            complement(6969..7472)
                     /locus_tag="PLU99_09380"
     CDS             complement(6969..7472)
                     /locus_tag="PLU99_09380"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO17308.1"
                     /translation="MLLRKGKSAFTLVELVLVIVIIGVLAAVAIPRLSRGSAGAGNAA
                     LATNLAMVRNAINLYAAEHNNTFPGPDAQGFVDKLTKFSDRTGTTVTTRDDSHPYGPY
                     LLAIPPCPVGENANKDTANKVLISTTSPPTPDPSSGEGWVYNATTGEFLPNTDAKDDA
                     GKLYKTY"
     gene            complement(7617..8159)
                     /locus_tag="PLU99_09385"
     CDS             complement(7617..8159)
                     /locus_tag="PLU99_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17309.1"
                     /translation="MSLTRRILIGILAVAVAGWVVDTLRSRTPQAAQAASATTEAAPA
                     EAANGEDLAALVQRLVSDHYAPVSAELERLERDLFVPTPLMQAETAPASEPAELSETV
                     EEVPPGHEFANTHLLTGVIIGHLPLAQVDGRLLPLGAVLDGHTLIAIERDFVVFERAT
                     DRERVALELKPRSAGAGERP"
     gene            complement(8165..8746)
                     /gene="pilO"
                     /locus_tag="PLU99_09390"
     CDS             complement(8165..8746)
                     /gene="pilO"
                     /locus_tag="PLU99_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOO17310.1"
                     /translation="MILCRRPFTVYHVDALGGLGLLAVALAGWWLAVVPWRQTCASYR
                     ELGQRRAALELELQSESAKLEGIRQELAWLQGVVDSQTLVVPRADSVPQLLREMTELA
                     SETEIELLSVVPQPAVAEGAYVVSDIQLTGRARSISLIRFLDQLAQRNPFQALMSCSV
                     KRPAGATHEICDVCWTLRLYMLPAAGSEGGALN"
     gene            complement(8743..9375)
                     /locus_tag="PLU99_09395"
     CDS             complement(8743..9375)
                     /locus_tag="PLU99_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOO17311.1"
                     /translation="MSCSVNLVPVARLQAHALARRRRAWIGLCTAGGLLVGLSWGAQN
                     VTRRAVTHLEGRAEALGVQHSLIQSQVSAAQEQRDGVLARLKLISAARRGQPWAGRLA
                     RLTRSAPDGVFLTQMTVSPRAEPQSAGPRAGAISSAQAPAPRPSTSPAHDVRLEGLAL
                     DHTGLIQLLHTLQNSADWENVELVRATTEPYAGRSVVAFEFDCRTAELAK"
     gene            complement(9372..>10046)
                     /locus_tag="PLU99_09400"
     CDS             complement(9372..>10046)
                     /locus_tag="PLU99_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17312.1"
                     /translation="FYLRRIDVSPCALVALACRTAPPAAGEIWGVLDLGLRHSILTVV
                     IGSVPAYVRVLSVSAHDWTCELARAFEIDYAAAEQVKREYGVGTLASGVPWPAGPPAG
                     EMRGAARPAAGTLLNAADLPTAVSTALRRSLRTLAHEVGRCFSYVVQNYPDLVLSRLF
                     LAGGGAASVGLAAALEAALDMPVRVISADPGRAAANGHDSENPAEAAPAFAAAMGAAL
                     LDLENS"
BASE COUNT         1858 a         3224 c         3196 g         1768 t
ORIGIN      
        1 gaccgagcgt gcggaagcaa gcttcctcgc cagctccgct acggcgttca atgcggcgat
       61 tgggggcgca tgtcagcagt atgctgatca agttcgtgct ggagagctct gatgtcagaa
      121 cttttgcggc tgtggaagac ggagttgtgt atgtgtcagg tatgcgccca tctggttgtg
      181 gttgtgactg tccccaatta tcgtccctaa ttatccaatt atagatgcga aacgagaaga
      241 acctccggcg tgtcggtgag cgaagtgcgg cctgaacaag tgggccaaga gcgcggcggc
      301 cacgttgtgt ggccgccgcg cagaatcacc gaaattagag attagccgcg gcgccgcagg
      361 agcaggccgg ccgcaaggaa cagccccagc gcggcaggct ccggaatgta ctggaagttc
      421 gggccgaagc ggcagtacgt cgtcgaggtc gtaggaccgg gcatcgccgg tgccggcgcg
      481 ccgccgtaac gcgcttggac gtatgtgatc gccggatcaa tcaccaggtc ggtgggtgcg
      541 gcgtagacca tccggtcctg gttgctcgca ccagtgaaga agccgatgac gtaatccacg
      601 ccgggaacca gcaccacgtc ctgcacatcc acgtagcgga aatgattaat cagcggatcg
      661 gtggcgccct ggctgatcac gccggaggtc agcagcgtgc cgctgtcgag ctcccacagg
      721 ccgatcgggt gctcgatatc catctcaggc tgatggccgc cgaatcggcg aataccggct
      781 ggcccatcag caaagcggcg caggccagag ccgcaatgct aagaatcctc ctcatcacac
      841 tctctcctcc aaaaaatgga ctgcagtctc gccacggcgg actcacgccc gcgccggggg
      901 gcgaacctgg acgggtaata cgagcagcat actccgatat tcgtctctgt acaacgcaat
      961 ttcacgcaga tttcgcgtcg cctcataatt cataattagg gacagtcacg tgactgtccc
     1021 taattagaag ctcgaaaggc gcgcgatcca ggcgatcagg ccgcagacta cgaagccgag
     1081 cgtgagtggg agaaatgcgg atacagccgt ccatttggcg ctgcccgttt ccttgtaaat
     1141 ggtgtagagc gtggtgctgc acggattgtg cagcaggctg aagagcatca ggtttacggc
     1201 cgtcaacgtc gtccagccgc cggccgtcag gagctgtcgt actgcctcgt gtgactcgag
     1261 ttcgaacatc acgcccgccc ccgcgcccat gtccgatgcc ccggtcacca tcaccgtcaa
     1321 catcagcacc gtcggaatga caatctcgtt ggccgggatc gcgatgatgt aggccagcag
     1381 aatcaccccg ttcagcccaa tgaaaacgcc cagcggatcg gcaacacgga tgaagtgccc
     1441 ggccaggctg tcgccgccga cgtgaacatt ggcgaccagc cagatcaccg cgccggccgg
     1501 cagcgcaaac accaccgcgc gccatagcac aaagagcgtg cggtcaatta gcgaggtgta
     1561 cagcgtctgc aacagccgcg gcggacgata aggcggcagt tccaggctga acgtgctgac
     1621 ttcgccctta agcaccgtgc gcgagagcgc ccacccgact acaaacgtca gcaagacgcc
     1681 cagaagagcc acgcccgtca ctgcggcggc agataccagg cccgccagcg acgccggaac
     1741 cagcgcgccg agaaacacgg tcgcaatcag aatctgggcc ggccaccgcc cgttacagag
     1801 cgcaaagttg ttggtaagaa tcgccagcag ccgctcgcgc gggctgtcga taatgcgtgt
     1861 ggccacgacg cccgcggcgt tgcagccgaa gcccatcatg gtagtcatgg cctgcttgcc
     1921 gtgcgccccg gacgccttga acagacgatc caggttgaac gccacgcgcg gcaggtaccc
     1981 gaagtcctcc agcagcgtga acagcgggaa gaagatcgcc atgggcggca gcatcacgct
     2041 gaccacccag gccgtgccga ggtacatgcc atcgacgatc aggccggtca accaccacgg
     2101 cgcgccaacc gcgccaaaca ggcccttaag ccaggggtga ccgtagtcaa tcagcacgga
     2161 cgcgatcaac gccgaaggca cgttggcgcc cgcgatcgtg atccagaaca tggccgcaaa
     2221 cagcaggatc atgatcggga agccccacac gcggctggtg agaatgcggt ccagcgtgcg
     2281 gtcgaaggtg ggccgggccg cgtggccgcg gcgtgaaacc gctcgttccg tgaggcgcgc
     2341 cgcctggccg tagatcgctt cgacgatgtg ctcgtgcaga tcgccgggga tctgttgtcg
     2401 ccaatgttgt gcggccgcca gaatcgcctc ggcttgcggt gttacagaat cgcgtttggc
     2461 cgtgtccatt gtgttcccga atcatccatg agtgccggcg ggctgcggcg ctgccagtgt
     2521 ttgcagcgtg gcgagttgac cgctgcgcaa ggcttccacg atggaatcgt cgccaccgag
     2581 caggcgcaac gcgacccagc gcggattggg caggtcgggg tagacggctt tgagccgggc
     2641 caccaggtcc gccagggccg gcttgatcgc ggcaggctcg gtttcgagcc gccgcggccg
     2701 gcacgccagc ttgcccgtgg ccacgtcgct gatggcttgc agcagttcgg gcaatccctg
     2761 gccgaagcgg gcggaggccg gcacgaccgg cacgccgaga tcgcgcgcga gctggcgctc
     2821 gtccaccgtg atgccgtggc gtttggcttc atccaggagg ttgaggcaga cgacggcacg
     2881 gtcggtaatt tcgaggacct gcaagacaag gttgaggttt cgctccagac gcgtcgcgtc
     2941 ggccacaatc acggtcactt ccggctgtcc gaacaggatg aagtcgcgcg cgatctgttc
     3001 gtcgaggctg gtcgacagca gcgagtaggt gccgggcaag tccaccagct tgaatctttt
     3061 gccgtcgaaa gcgtagccgc cctcggcgcg cgacaccgtc ttgcccggcc aattgccggt
     3121 gtgctgacgc aggccggtga gcgcgttgaa gaccgtactc ttgccggtgt tgggattgcc
     3181 cgcgagcgcc acgacgtaat cccaggaatc cagggccacg ccgagctttt tgagattggc
     3241 ggcgcgatag accgggcagg acgcgcacgc ttcctttgcc gccacgggcg cgacgggcag
     3301 gggcgacgca gcagaagaat tggcagaatc ggcgcgtttc atgtggcgac cgcctcccgc
     3361 gagttgatgc ggaccagatt ggcctgttcg cgccgcaacg ctaccaccga accgcgcaca
     3421 cggtaggccg tcgggtcacc tgccgggctg accatctcca cctccaccgg cgtgcccgga
     3481 acaaaaccca ggtcgagcag gcggcggcgc tccggccccc ggcacgcgcc cgagaggccg
     3541 accacgcgcg cacgccggcc aggcggcagc ccggagagga actcctcttc aacgaggtcc
     3601 gccgtgccgt agtccggcag cggtctaatc gagacgttct ccgccagcac cggcgccagc
     3661 gtgtgttcgt tgccgtccgc ccagaaccgt attctcgtcg gggttttctc aatgacgaag
     3721 gcgtgcatgc ccgcccgtaa ccccagagcg accagttggc tgtaaaccgt ctccggctcg
     3781 tcctcaatgt gcgtaatcag caccggcgtt tcgggctcaa cgctattgag cgactggcta
     3841 tcttcgcctt cgaaagtgcc gtccgaactg gggatcacgt ccccgtgcgg gtcacgtgtg
     3901 gggtgcccca ggcgggcggc caaggcgcgc gtttgcgccg gcgtcagcaa gtgctcctgc
     3961 ctctcggcgc gccggtgcca ttcgtcctcc gcgatacccg tttcttccgc gagatagctt
     4021 tcccacagcc ggtgcgcgcg gagaacgtgc aggcccatct cgcgtcccgc cggccgcagc
     4081 cgcagttggc cgccttcgaa ggtcagcagg tcgtggctgg cgagctcatc cagcaggcgg
     4141 gcggcgctgc cggtggtgat gtgcagcgcc ccggcgatgc tctccagcgt gggcacgcgc
     4201 ccgttggcct cactcttgag aatgtgcttg agggcgtctt cccggcgcat gcggcgagcc
     4261 agatcatgag cctgacgcca gcgcgccagc aggccgtgcc gcggccagaa gacagccacc
     4321 gccagcaaga tcagaatgag gtaaacagca gtcactcgca aaggttccct gatagcgacg
     4381 aacagccggc cgccgtgtcg ccgaagaccg tcgctcacgt ttgggtgtgg gcggcggccg
     4441 gcggcagcat gcccgtcgca aggttccgca ggggcaatgc catcgcgccg gcgttcttca
     4501 gggattgtag ccgtggtggg gcccgggaag tactgccacc gctgccgcga agcccgaggc
     4561 cgcgctgatc tccgccccga caccgccaat tccgggtctg acgggctact aacgctcaaa
     4621 aaaatcttgc aaaagccttt gttgacagta cgatagcagt gccccgcgcc gcgctagtgg
     4681 gactagccgt ggcggcggag gctggggaga aggctaagga ggttcagccg tgctgttcgt
     4741 ttcaaccctg attctggtga gttaccctgc gctcgtggcg accgtgccgc cggcacgttc
     4801 gctcaactat gtggactcgt cgtccggcct gcagtttccg gagatgtatt cgggccccac
     4861 ggagctcgaa tttgccgacg tcaatggtga cggacacgta gatatcggga tggtggggga
     4921 tcacgattcg ccgggtatgt ctcagcatgg cattacggtt tggctcggag acggcgcggg
     4981 gcactggtcg gctcgccaga gcggctattt cggctatgga ggcatcgcct tcggcgacgt
     5041 taacggtgac gggctgatgg acgtcggcta cgccatgcac cacaccgacc ccggcagcga
     5101 ttttggcgac caattaatcg aagtcgcgct gggcgacggc actggcgaga actggattcc
     5161 gtgggacgac ggtctggcca cgagcggcga gacatggggc atgttcggga cggatttcgc
     5221 cgacgtcgac aacgacggcg acctggacat cggttcgata tctttcggct gctgcgcggg
     5281 gttgcacgtt tatctgaaca acggcgatgg cacctggacg cagagttggg gcgtccttga
     5341 cggagacgcg tacgagatct tccagttcgg agacatcaat ggagacggct tcatcgattt
     5401 cgccgcggcc cacagctacg gcacggtgtg gctgggcgat gggcagggca atttcacggt
     5461 cgccgactac ggcctgcccg gtggagtcgg tgattggcgc gaaggggtgg ctctcgggga
     5521 tgtaaatggc gatgggcgcg acgacctggc gttccgaaat gcgaacggcg gaatagacgt
     5581 ttggtcttac gtgggccccg gcgtctggca aaatctcagc gggacgctgc ccgccagcgg
     5641 caatttctac tacacgcaga tcgcggacat gaacctggac ggccacggcg atatcgtagc
     5701 ttgcgcccgt gggcaaatca gggtctatgg gggtgatggg gcgggaaact gggagttgct
     5761 gaccacggtg aacatcggcg gctccggcgc gaaggccttc cgtgccggcg ttgacgccga
     5821 ccacaacgga taccccgaca ttgtgatggt caatcgggag cccaacaacc gtgcgcggtt
     5881 ctatcgtgag accacgcccg tctccagcac gtgggtgtat cccaagtatc cgcgcggcgg
     5941 tgaagtcatg tatgccggct cggtgcgatt catcgactgg catgcagcag tcgccaacgg
     6001 agaatcaaca gttaccatag agttatcggt ggttgggccg gatgggccgt ggatcgaaat
     6061 cgcctccaat gtgcccaaca acgggcgctt tcagtggcat ctgccgcggc acctcaccag
     6121 ctcaaatact tgctatttgc gctacacgct cggagaggcg caggctgtca cgcccgctcc
     6181 gttcactatt gtcggtgaac ctggcatggc gccgctgagc atcagtctgc cggaaggtac
     6241 gccggagttt ctgtcgccgg ccgcgccgac ccggttcacc gtcgagatcg taagtggggg
     6301 agaggcctac gtgcctggtt cggccttgct gcattactgc tatgatggcg gggagttcat
     6361 caccaggccg ctgacgccgc tgggtgaaac gctctatcag gcgacccttc ccgctcccgc
     6421 ttgtgctgat acgccgcaat tctacatcag cgccgtcggc gacggcggat ccaccgttta
     6481 cagcccggag ggggctcccg agaatgtcta caccgcccgt gtgggcgaag tgagggccct
     6541 gctggtcgat gacttcgaaa ccgacccgtc ctgctatgat cgtggcgacc tgaactgcga
     6601 tggcgcgctg aacgtgttcg acatcgacgc cttcgtgctg gccctgacgg atccgctgga
     6661 gtacgcgacg gcgctgcctt attgcaactg caagctgggg gacatcaact gcgatgaggc
     6721 cgtgaacgtt tttgatatcg atccatttgt cggctgtctg accggcggcg gatgtgctcc
     6781 atgtccctaa gccggtttga atgccgggcg ctatgctgaa ggtgagcgtg ggccgccccg
     6841 aagcaacaaa agccggccct ttcgggccgg ctttgaatgc tcgtgcttag ccgtactagc
     6901 ggtgggtgtt cagggagaat tctcggcggt agcgccgcgg ttctgactcg gttgaacgtc
     6961 ctggttgtct agtacgtctt gtacaacttt cccgcgtcat ccttggcgtc ggtgttgggc
     7021 agaaactcgc ccgtggtagc gttgtatacc cagccttccc cactggaggg gtccggcgtc
     7081 ggaggactag tggtggagat caggaccttg ttggcagtgt ccttgttggc gttctcaccg
     7141 accggacatg gaggtatggc cagcaggtag gggccatagg ggtgcgaatc gtcccttgtc
     7201 gtgacggtcg tgccggtccg atcgctgaac ttcgtcagct tgtccacgaa accttgtgcg
     7261 tccgggccgg ggaaagtgtt gttgtgttcc gcagcgtaca ggttgatcgc attgcggacc
     7321 atcgccagat tcgttgccaa ggccgcgttc ccggcgccgg cggaaccacg gctcaggcgt
     7381 ggaatggcca ccgccgccaa cacgccgatg atgacgatca cgagcaccaa ttccacgagc
     7441 gtgaaagcgc tcttgccttt acgaagcaac ataacaacct ctccagcttg tagtgggcaa
     7501 accccggccc ggcggatgat ccgcagtgtc ttttgccaca ttgctatcgt ccaggccggc
     7561 aggtcacttt aaatgacatg aacctgctct cgggcgccgc caatggtgcc cataagtcat
     7621 ggcctttcgc ccgctccagc gctgcgcggc ttgagctcga gcgcgacccg ctcgcggtcc
     7681 gttgcgcgct cgaaaaccac aaaatcgcgt tcaattgcga tcagcgtgtg gccgtccaga
     7741 acggcgccca gcggcagcaa gcgcccatcg acctgcgcca gtggaagatg gccgataatg
     7801 acgcccgtca acaaatgtgt gttcgcaaac tcatgaccgg gcggtacctc ctcgaccgtc
     7861 tcagacaatt cagccggctc ggaggccggt gccgtctcag cctgcattag cggagtcggc
     7921 acgaagagat cgcgctcaag ccgctcgagc tcggcggaaa cgggcgcgta gtgatcagaa
     7981 accagccgct gcactagcgc cgccagatcc tcgccattcg cggcctccgc gggcgccgcc
     8041 tcggtcgtcg cggacgcagc ctgcgccgcc tgcggcgtcc gactgcgcag cgtgtccacc
     8101 acccagccgg cgaccgccac cgccagtatc ccgatgagaa tcctccgggt gagcgacacg
     8161 ctcttcaatt cagggcccct ccctcagatc ccgccgccgg cagcatgtaa agccgcaacg
     8221 tccagcatac atcacagatc tcgtgcgtgg ccccggcggg acgcttcacc gagcagctca
     8281 tcaacgcctg aaacggattc cgctgcgcca gttggtccag aaagcggatc aagctgatgc
     8341 tgcgagcgcg gccggtaagc tgaatgtcgc tcaccacgta ggccccttcg gctacggctg
     8401 gttgtggcac gacgctcaac aattcgatct ccgtctccga ggccagttcc gtcatctcgc
     8461 gcagcagttg cggaacggaa tcggcacgcg gcacaaccag cgtctgcgag tcaaccacgc
     8521 cttgcagcca ggccaattcc tgccgtatac cttccagttt cgccgattcc gactgcagct
     8581 ccagttccag tgcggcacgc cgctggccaa gttcacggta gcttgcgcaa gtctgccgcc
     8641 agggaacgac cgccagccac cagccggcca gcgcgaccgc cagcaggccc aggccgccca
     8701 aggcgtccac gtgatagacc gtaaatggcc ggcggcagag gatcacttgg ccaactccgc
     8761 ggtgcggcaa tcgaattcaa atgcaacgac gctgcgccct gcgtagggtt cagtggttgc
     8821 ccgcaccagt tcgacgttct cccaatcggc agagttctgc agcgtgtgga gcaattgaat
     8881 cagccctgtg tggtccaacg ccaatccctc cagacgcaca tcgtgggccg gacttgtcga
     8941 tggtcgcgga gccggcgctt gggcagaact gatcgctccc gcccgtggac ccgcactctg
     9001 tggctccgcc cgagggctga cggtcatctg agtcaggaaa acaccgtccg gggccgagcg
     9061 cgtcagccgc gctaaccgtc cggcccaggg ttgtccgcgg cgcgccgcgc ttatcagttt
     9121 caagcgtgcc agtactccgt cccgctgttc ttgcgccgcc gacacctggc tctgaatcag
     9181 gctgtgctgc acgcccagcg cctcggcccg cccttccagg tgcgtgacgg cgcggcgggt
     9241 tacgttctga gcgccccagc tcagccccac cagcagccct cccgccgtgc atagaccgat
     9301 ccacgcccgg cgtcgcctgg ccagcgcgtg cgcctggagc cgtgctactg gaacgagatt
     9361 gaccgagcag ctcatgagtt ctccaggtca agcagcgccg cgcccatggc cgccgcgaac
     9421 gccggcgccg cttcagcggg attctcgctg tcatgcccgt tcgcagccgc acgccccgga
     9481 tctgcactga tcactctgac cggcatgtcc aaggccgcct ccagcgccgc cgccagcccc
     9541 acggaagccg ccccgccacc ggccaagaac aaccggctca gtacgaggtc cggatagttt
     9601 tgcacaacgt atgagaaaca gcggcccact tcgtgtgcca aggtccgtag cgagcggcgc
     9661 aaggcggtcg aaaccgccgt gggcagatcg gccgcattca gtagcgttcc cgcggccggg
     9721 cgcgcggccc cgcgcatttc gcccgccggt ggcccggccg gccacggcac cccggaagcc
     9781 aaagttccaa caccgtattc gcgttttact tgttcagccg ccgcgtagtc tatttcgaaa
     9841 gcccgagcaa gctcacacgt ccagtcatgg gccgaaaccg acagcacccg cacataggcc
     9901 ggaactgatc cgatcaccac cgtcaatatt gagtggcgca aacccaggtc gagcacaccc
     9961 cagatttcgc ccgccgcggg cggcgccgtg cggcaggcca gtgccaccag ggcgcacggc
    10021 gatacgtcaa tccgccgcag gtaaaa
//
LOCUS       DAOSYS010000133         9934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5799_length_9934_cov_5.255694, whole genome shotgun sequence.
ACCESSION   DAOSYS010000133 DAOSYS010000000
VERSION     DAOSYS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_9934_cov_5.255694"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PLU99_09405"
     CDS             complement(<1..885)
                     /locus_tag="PLU99_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOO17313.1"
                     /translation="MASVSTGEAAAPVRDLSWVDAVAVVMGIVIGSGIFITPALVAGN
                     TTGLGTMLAAWLGGGILCFCGALSYAELATAYPHSGGEYVYLSRAYGKAVGFFFVWSR
                     MTVIQTGSIAAAAYIFGRYANRLLPLGPSGPLLYALGAAVVLTGMNAVGLRAGKWTQN
                     LLTVTKVLGIVAIILVGAFVSPSVPPVPVEEAQTTSTPAFGLAMVFVLYTFGGWSEAA
                     YVAGEVRQAQADMVRALVFSMFGLTALYVAINLAYFHILGFEGMRASDAIAADTIAAT
                     LGEPGSLAVSVLVAIAALG"
     gene            complement(1134..3341)
                     /locus_tag="PLU99_09410"
     CDS             complement(1134..3341)
                     /locus_tag="PLU99_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17314.1"
                     /translation="MTTHPDGCPAFARRKTCALAIAASLAGLVDLGCGPLDFIPLRDA
                     ARLRANVPADLDAENAGVIGRFDETPDWGDSSAVDRESQREQAPHPALPRVSGRVVNA
                     ATGAGLSEVVIHGLPHHPATDGDGNYEAVVTPGWSGAATPQRPGFIFSPPSRTYAEIE
                     DTQAGQDYVATAAAVLYVLPSAGLISSGPQGGPFDPVSRTYQLVSIGTQPLDWWASHR
                     RPWVSLSQTSGTLARGENTTVTVSINGDAAALGIGTHWDTVEFTNLTTGAGNATRMVQ
                     LTATGPGWVSSWTNRGFDVEVTGTFGVHRWDAYTNDRYVSVPRGATPTITVTRNADLR
                     RADHSPSPDDTTRVHIGLLTIGRQQFWTPDWDMYEPAGFVYEWPFPVGPVTEPTPVQI
                     WNQHPGCYFESSDWLAYGSNAMEIWLLPHDLDPGNLPPVDYANGRLPAGQLVSAPPGV
                     WPALYRDAADEMRLHAAAADLGATLYGDGGPYDDSSYGSYWPDVAEWAARNALIGWHR
                     NSSAHLYVARQQLLAVLSVRTFTNECAFWSRARCVPNADFMTSYGRELSNMFFEAMVA
                     ACVLGPNMTDTLNVDELEAVNAHLYRFAVHDIFASLTSEQYYSANGGINLHYHPICVV
                     AGWNWNIPEAVSIADYLPEPTHTECSYFIDEHGIGQYALRYCQLHSPEDKDYEHGGNS
                     QSVCFWVRFSEFFKQSIDVYAAERYVIDQFSGLSIDCWDALDVLMDGWQAPAK"
     gene            complement(3545..4681)
                     /locus_tag="PLU99_09415"
     CDS             complement(3545..4681)
                     /locus_tag="PLU99_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO17315.1"
                     /translation="MFRPKSRSIVPAADRRAFTLIELLVVIAIIAMLIGIIMPGLSQA
                     RDQARRVATRGTMKAIGDSLEVFIGENEDECRGQSYPPSGGGIYYDDPTEEGGAPVDG
                     QVPLGQEQIFGSQWIVRYLMGKNLDGYVAARNVPSAFNEVAEDGWRQKSWYGSGADGE
                     EWPDYPKEPLPRVGPYMNNPPIKAPRDLQGGAMIPTEERLLPKYSNWVFVDTFGTPIL
                     YYAAKSGYANRPDAIQTTYGHPDHVDNSPGIYNWRDNALFTGMTTADPDTGAIETEGN
                     MRPWDFGAGPHKLTFGPPEWRNEQVTGSAWRQAKQRVDLRKAPFSFAYQILNKQAFET
                     SVAQQGTSPDMSKVTIIPARRDSYILWSPGKDAVFGTNDDVVNW"
     gene            complement(5258..5932)
                     /gene="pncA"
                     /locus_tag="PLU99_09420"
     CDS             complement(5258..5932)
                     /gene="pncA"
                     /locus_tag="PLU99_09420"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008200324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HOO17316.1"
                     /translation="MRDALILVDIQNDFLPGGALPVPDGDQVIPVANRLAKHFDLVLA
                     TQDWHPPEHVSFAANHPGRKPGDVVYIDGQEQVLWPVHCVQDTPGAQFPPALDTSRIT
                     QVFRSKGADPAVDSYSGFFDNARKHATGLEDFLRKAGVQTVYLLGLATDYCVPATALD
                     AVSLGFQTFVVEDGCRAVDLKPGDGRRALERMRSAGVQVVRSDQVIASRAARTPKTSN
                     KASPTR"
     gene            complement(5932..7440)
                     /locus_tag="PLU99_09425"
     CDS             complement(5932..7440)
                     /locus_tag="PLU99_09425"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOO17317.1"
                     /translation="MLPSCLYRPSLAALTDLYELTMACGYFKAGVSEREAVFNLTFRT
                     SPFNSGYAVACGLAYVVDSLRHFHFANEDIEHLATLRGNDGRPLFEPRFLEYLRELRL
                     ACDVDAIPEGTLVFPHEPLIRVRGPVIHAQILESAVLNFINFQTLIATKAARVCWAAQ
                     GDPVIEFGLRRAQGIDGALTVARAAYVGGCTSTSDVLAGRLFNIPVAGTHAHSWIMVF
                     DSELEAFEAYAQALPNNCTFLVDTYNTLAGVRHAVEVARRLRARGHEMIGIRLDSGDL
                     AYLSIEARKILDAGGFPNARILASSELDEHVIASLKQQGAKVNVWGVGTRLVTAYDHP
                     ALGGIYKLTAIRKPGQDWEYKLKLSEQVVKTSTPGIHQVRRFHRDGQFMADMIYDERL
                     GIAADCTIVDPADATRRRPIPPNTPYEDLLVPIARAGQVVYEPPPLEETRRRTQSQLG
                     CLHPTIKRLINPHEYPVGLERSLHELKTRLILEARGLGGGEVGKWESGKVGR"
     gene            7565..8287
                     /locus_tag="PLU99_09430"
     CDS             7565..8287
                     /locus_tag="PLU99_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17318.1"
                     /translation="MKHGYWGALLALAAGISAAPSQASAEPFVLRYDPGYMFPEQEGW
                     ERHTHDPEGLLSREVDGTVLRLDTSGSLSMPDFYYADCPGLDPTLGEELRVAWRMRTL
                     ETQPDYLWSDVGITVSGLSSGYAVITVGPDYVSQEYGQGGLPEHVYYFTAGAMHQFEV
                     RTQDMATFSLYVDETLAFASNFDRPAETGPFRVYFGDAVSGRRSVSEWDYVQVAVVPE
                     PAGLCAAAVALLVVLQVRKGIR"
     gene            complement(9035..9340)
                     /locus_tag="PLU99_09435"
     CDS             complement(9035..9340)
                     /locus_tag="PLU99_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO17319.1"
                     /translation="MSNRLYVGNLSPGMTSDQLQALFSDYGTVNFAQVVADRDSGRSE
                     EFGFVEMATVEEAEAATAALNGRMLADRCLIVEPARRKPGARDRGYHGGPHLWNAQD"
BASE COUNT         1873 a         3124 c         3047 g         1890 t
ORIGIN      
        1 ccccagagcc gcaatcgcca ccagcaccga gaccgccagg ctgccgggct cgcccagcgt
       61 ggcagcgatc gtatcggccg cgatcgcatc cgaggcgcgc atgccttcaa agcccagaat
      121 gtggaaataa gcgaggttga tcgcgacata cagggccgtc agtccgaaca tgctgaacac
      181 cagggcgcgc accatatccg cctgcgcctg ccgcacttct cccgcgacgt aagccgcttc
      241 gctccatcct ccgaacgtat acaacacgaa caccatcgcc agtccgaagg ccggcgtact
      301 ggtggtctgc gcctcctcca ctggcactgg cggcacgctg ggactgacga aagcgcccac
      361 caggatgatc gcgacaatgc cgagcacttt tgtgaccgtc aacagattct gcgtccattt
      421 gccggcccgc agtcccacgg cgttcatgcc cgtcaacaca actgccgcac ccagtgcata
      481 gagcagcggc cccgacggac cgagcggaag caggcggttc gcatagcgcc cgaatatgta
      541 agccgcggcg gcgatcgagc cggtctgaat taccgtcatt cgcgaccaga cgaagaaaaa
      601 gcccaccgct tttccgtacg cgcgtgacag atatacatac tcgccgcctg aatgcggata
      661 ggcggtcgcc agttccgcgt acgacagcgc gccgcaaaag caaagaatcc cgccgcccag
      721 ccaggccgcc agcatcgtgc ccagccccgt ggtgttgccg gcgaccaggg ccggcgtgat
      781 gaagatgccg gagccgatga cgatccccat gacgaccgcc acggcgtcga cccacgaaag
      841 gtcgcggaca ggtgcagcag cctccccggt tgaaacgctc gccatactaa gtttccagag
      901 acgcgcggat aaccggcggg gcacacgccc gcgcgacgat gctgcggcgg ccgctcgcgg
      961 gaagcactat ccacccgcaa ccttagagga taggtgtgct ttcggcgcat ggccaatcag
     1021 gatgtctctt gattgacacc taacggccga acccgaaaca gcttatgtca ttacagagat
     1081 acgcgccggc gttgcccgct tctcgatctt tcgatccccg gatttctgat ccctcacttc
     1141 gccggcgcct gccatccatc cattaaaacg tccagcgcgt cccagcagtc gatggacagc
     1201 ccggagaact ggtcgattac gtatcgttca gcggcgtaga catcaatcga ctgcttaaag
     1261 aattcggaaa agcgtaccca gaaacagacc gactgcgaat tgcccccatg ttcgtagtcc
     1321 ttatcctccg ggctgtgtag ctggcaatag cgcagcgcgt attgtccaat tccatgttca
     1381 tcaatgaagt agctgcactc cgtgtgcgtc ggttccggca aataatccgc aatactcacc
     1441 gcctcgggta tgttccagtt ccagcccgcc acgacgcaga tggggtgata gtggagattg
     1501 atgccgccgt tggccgaata gtactgctcg ctcgtgaggg aagcgaaaat gtcgtgaacc
     1561 gcaaaacggt ataaatgagc gttaacggcc tccaactcgt ccacattcag cgtatcggtc
     1621 atgtttggcc cgagcacaca cgccgccacc atggcttcaa agaacatgtt ggatagttcg
     1681 cggccgtaac tggtcatgaa gtcggcgttc ggcacgcagc gggcgcgaga ccagaaggcg
     1741 cattcgttag tgaacgtgcg cacgctcagt actgcgagca gttgctgccg cgctacgtac
     1801 aagtgcgcgc tgctgttccg gtgccagccg atgagtgcgt tccgggcggc ccactcggcc
     1861 acatccggcc agtaactgcc atagctactg tcgtcgtacg ggccgccgtc gccgtacagc
     1921 gtggccccca ggtccgcggc cgcagcgtgc aaccgcattt cgtcggctgc gtcgcggtac
     1981 agcgccggcc agactcccgg cggggcgcta accagttgcc cggccggcag ccgcccattc
     2041 gcataatcga cgggcggcag gttaccgggg tccaagtcgt gcggcagcag ccaaatttcc
     2101 atcgcgtttg aaccgtacgc cagccaatcg gaggattcga aataacagcc aggatgctgg
     2161 ttccagattt gcaccggagt cggctcagtg accgggccga cggggaacgg ccactcgtaa
     2221 acgaacccgg ccggctcgta catgtcccaa tcgggagtcc aaaactgctg gcggccgatg
     2281 gtcagcaacc cgatgtgaac gcgagtagtg tcgtccgggc tgggcgaatg gtcggcgcgt
     2341 cgcaaatccg cgttacgggt gaccgtaatt gtcggcgtcg cgccgcgtgg cacgctgacg
     2401 taacgatcgt tcgtgtacgc gtcccagcga tgaacgccaa aggtcccggt cacctccacg
     2461 tcgaacccgc gattcgtcca ggaggaaacc cagcccggac cggtcgccgt gagctgaacc
     2521 atgcgcgtcg cattccccgc gccggtcgtc agatttgtga actctaccgt gtcccagtgc
     2581 gtgccgatgc cgagtgctgc ggcgtcgccg ttgattgaga cggttaccgt cgtattctcg
     2641 ccccgcgcca atgtgccgct agtctgcgat aggctcaccc acggccgcct atgacttgcc
     2701 caccaatcca gcggctgcgt gcctatgcta accagttgat acgtgcggct gaccgggtcg
     2761 aacggcccgc cttgcggccc gctgctgatc agaccggcgg acggcaggac gtaaagtacc
     2821 gcggccgccg tcgcaacgta gtcttggccc gcttgcgtgt cctcaatctc cgcgtacgtg
     2881 cggctgggcg gcgaaaagat gaatccaggc cgctgggggg tagcggcgcc gctccagccg
     2941 ggagtgacta ccgcttcgta attgccgtcg ccgtcggtgg cggggtggtg cggcagaccg
     3001 tgaatgacga cttcgctgag accggcgccg gtcgcagcgt tcaccacgcg gccggagaca
     3061 cgcggcaggg ccgggtgcgg tgcctgttcc cgttgagact cgcggtctac ggcgctgctg
     3121 tcgccccagt ccggcgtttc gtcgaaccgc ccaattacgc cggcgttttc cgcatccaaa
     3181 tcggcgggaa cgttggcccg gagccgcgcc gcgtcccgta gcggaatgaa atcaaggggc
     3241 ccacagccaa ggtcgacgag gcccgccaga ctggccgcta ttgccagggc acaggttttc
     3301 cgccgtgcga atgcaggaca gccgtcgggg tgcgtcgtca tccggccacc ttccaaaacg
     3361 acacaggaat agcgcggtgt gcctgctaag cggcagggca agaaacgtta ggccctgtaa
     3421 tgctggcggg tctacccggc ggaaggtgta gccgtataca aaacacaacc ccggacgggg
     3481 tcgcccgggg tctgtgtcaa aaaatcttgt tgttctgtcg cgctatcagc gcgaccgggt
     3541 caaattacca gttcacgacg tcgtcattgg ttccgaaaac cgcgtccttg cctgggctcc
     3601 aaagtatgta cgagtcgcgt ctggccggaa tgatcgtcac cttgctcatg tcgggcgagg
     3661 ttccctgttg ggcgaccgac gtttcgaacg cctgcttgtt gagaatttgg taggcgaagg
     3721 agaacggggc cttccggagg tcgactctct gcttagcttg tcgccatgca gaaccggtaa
     3781 cctgctcgtt tctccactct ggtggcccaa atgtcagctt gtgcggcccg gccccgaaat
     3841 cccaggggcg catgttgccc tctgtttcga tggcaccggt gtctggatcc gcagtagtca
     3901 tcccggtaaa gagggcgttg tcgcgccagt tgtaaatacc cgggctgttg tcgacatgat
     3961 caggatggcc gtaggtcgtc tgaatcgcat cagggcgatt ggcgtagcct gactttgcgg
     4021 cgtagtaaag aatcggagtg ccaaaagtgt ccacaaagac ccagttggag tacttcggca
     4081 gcagtctttc ttccgtgggg atcatggccc ctccctgcaa gtcgcgcgga gccttgatcg
     4141 gcggattgtt catatatggc ccgacgcgcg gcagtggttc ctttgggtag tctggccact
     4201 cctcgccgtc ggcgccgctt ccgtaccaac tcttctgccg ccagccatct tcggcgactt
     4261 cattgaacgc gctcggcacg ttgcgcgccg ccacgtagcc gtcgaggttc ttgcccatca
     4321 ggtaccggac gatccactgg gagccgaaaa tctgctcttg tcccagggga acctgcccgt
     4381 cgaccggggc cccaccctct tcggtcggat catcgtaata gatgcctccg ccggacggtg
     4441 ggtaactctg gcctctgcac tcatcctcgt tttcaccgat gaagacctcc aggctgtcgc
     4501 caatggcctt catcgtgccc ctggttgcga ccctcctggc ctgatcgcgg gcctggctca
     4561 gcccgggcat gatgatcccg atcagcatcg cgataatcgc gatgacaacc agaagctcga
     4621 tcagggtaaa agcacgccga tcggcggccg gaacgatgct gcggcttttt gggcggaaca
     4681 taaccttttt ccctctgaga ggactccggt tatcgggcat ttaccaactc gggggcggat
     4741 caccgtcccg acgcaattct ggtctacggc gggtgtcttg gcaagtcgct taccccgcag
     4801 acggagaggg atcaaaagct cactggccat gcgggcccgg aaaggcccca taactgcaat
     4861 tgagagacgc tgcgacgtct gggcgttcgc ccgcatcggc cgcgccgaag aacgcctagc
     4921 cgagcattac gcttccgtca tttcgaattt aggtgatcgt catttgcggc ggttattttg
     4981 attttcgtat ccggagttcg aataagggaa ttcggaacag ccgcttggga agtgaagtta
     5041 caaatacggc cgccgctcgc cgaggtcctc acgcgatcgc cggccggccg ctacggtccc
     5101 cgatttcaac tttcgttttg cgtagttcgg gctgaagggt ggtgtgcgtg ggtcacgcca
     5161 cggaccgctg atggcgatgc aaatcgcttt cagcggttct ttatgcgcaa agagtgacgg
     5221 actgccgcca gagtagcact gagcgcgcct cgatcctcta tcgcgtcggg ctcgccttat
     5281 ttgaagtctt gggggttcgc gcagcgcgtg acgcgatgac ttggtcgctg cgaaccactt
     5341 gcacgccggc cgaccgcatg cgttcgagtg cccgccgccc atctcccggt ttcagatcga
     5401 ccgcgcggca gccgtcctcg acgacaaacg tctgaaagcc gagactgacg gcgtccagcg
     5461 ccgtcgccgg cacgcagtag tcagtcgcca ggccgagcag gtaaaccgtt tggacgccgg
     5521 cttttctgag gaagtcttcg aggccggtgg cgtgcttgcg ggcgttgtcg aagaagccgc
     5581 tatagctgtc gaccgccgga tccgcgccct tgctgcgaaa gacctgcgta atccggctgg
     5641 tgtcgagcgc cggcgggaac tgtgctccgg gcgtgtcctg aacgcagtgc accggccaga
     5701 gcacctgttc ctgcccatcg atgtagacca catcgcccgg ctttcgtccg gggtggttgg
     5761 cggcgaagct cacgtgctcc ggcgggtgcc agtcctgcgt cgcgaggacg aggtcgaagt
     5821 gcttcgccag tcggttggca accggaatca cctggtcgcc atcgggcaca ggcaaagccc
     5881 cgccaggcaa aaaatcattc tgaatatcaa caagaatcag cgcgtctctc atcaccttcc
     5941 caccttccca ctttcccact ttcccacctc cccaccgccg agcccccgcg cctcgagtat
     6001 gagccgcgtc tttagctcgt gcagcgaccg ctccagtccg acgggatact catgcggatt
     6061 gatcagccgc ttgatcgtcg ggtgcagaca gcccaactgc gactgcgtcc ggcggcgcgt
     6121 ctcctccaac ggcggcggct catagaccac ttgcccggcc cgcgctattg gcaccagcag
     6181 gtcctcatac ggcgtgttcg gcggaatcgg ccggcggcgc gtggcgtcgg ccggatcgac
     6241 aatcgtgcaa tctgcggcga tgcccaggcg ttcatcgtag atcatgtcgg ccatgaactg
     6301 gccgtcgcga tgaaagcggc gcacctggtg aattccgggg gtcgaagttt tcacgacctg
     6361 ttcggagagc ttgagcttgt actcccaatc ctggccgggc ttgcggatgg cggtgagctt
     6421 gtagatgccg cccagggccg ggtgatcgta ggccgtaacc agccgggttc cgacgcccca
     6481 aacgttgacc ttggcgccct gctgcttgag gctggcgatg acgtgctcgt cgagttcgct
     6541 gctggccagg atgcgcgcgt tggggaagcc accggcgtcg aggattttgc gggcttcgat
     6601 gctcaggtag gccaaatcgc cggagtccag gcggatgccg atcatctcgt gtccgcgggc
     6661 gcgcaggcgg cgggcgacct ccacggcatg ccgcacgccc gccagcgtgt tgtaggtgtc
     6721 cacgagaaag gtgcagttgt tgggcagggc ctgggcgtag gcttcgaagg cctccagttc
     6781 gctgtcgaag accatgatcc aactgtgcgc atgcgtgccg gccaccggga tgttgaagag
     6841 ccggccggcc agcacgtcgg aagtgctggt gcagcccccg acgtatgcgg cccgggcgac
     6901 ggtcagcgcc ccgtcgatgc cctgggcccg ccgcagcccg aactcgatca ccgggtcgcc
     6961 ctgcgccgcc cagcacacgc gggccgcctt ggtggcgatg agcgtctgga agttgatgaa
     7021 attcagcacg gccgattcga ggatttgggc gtgtatgacc ggcccgcgca cgcggatgag
     7081 cggctcgtgc gggaagacga gcgtgccttc ggggatggcg tcgacgtcgc aggccaggcg
     7141 cagctcgcgc aggtactcga ggaagcgcgg ctcgaacagc ggccgcccgt cgttgcccct
     7201 cagcgtggcc aggtgctcga tatcctcgtt cgcgaagtga aagtggcgta aggaatccac
     7261 tacgtacgcc agcccgcagg ccaccgcata gccgctgttg aagggtgaag tccggaatgt
     7321 caggttaaac acggcctcgc gttcgctgac gccggccttg aagtagccgc aggccatggt
     7381 gagctcgtac agatcagtca gcgcggcgag cgacgggcga tacaagcagc tcggcagcat
     7441 gcggcaccgt tacaagtttc cactacattt taaggtgcca atgcagagca gagccggggg
     7501 gttgcccgtt gcgtctcggc gcgtgaccgc ggccgggcac acgtctttcg aaaggagccg
     7561 aaaaatgaag catggttatt ggggagcgct gcttgcgctc gcggcgggaa tttcagcggc
     7621 gccgtcccag gcgtccgctg aacctttcgt gctgcgttac gacccgggct atatgttccc
     7681 ggagcaggaa gggtgggaaa gacatacgca tgatcctgaa gggctgctta gccgcgaggt
     7741 cgacggcacc gtcctcaggc tggatacaag cggctcgctc tccatgcccg atttctatta
     7801 cgctgattgt cccggcctcg atccaaccct gggcgaagag ctgcgcgtgg cctggcgcat
     7861 gcgaacgctc gagacgcagc cggattactt gtggagcgat gtgggtatca ccgtttcggg
     7921 tctctcatcg gggtatgcgg taatcaccgt cggaccggac tacgtcagtc aggagtacgg
     7981 tcaaggcggt ctgccggagc atgtgtatta tttcaccgca ggcgccatgc atcaattcga
     8041 ggtccgcacg caggatatgg caacgttcag cctatacgtg gacgaaaccc tggccttcgc
     8101 gagcaacttt gacaggccag ccgagaccgg cccgttccgc gtatactttg gggatgccgt
     8161 cagcggcagg cgatccgtgt cggaatggga ctacgttcag gtggcggtgg tgccggaacc
     8221 ggctgggctc tgcgccgccg ccgtggcatt gctcgttgtt ctccaggtta gaaaggggat
     8281 acgctgatgt gtctactgct aaagtgtgcg cttccattcg ccatctgcct tgccttaggt
     8341 tcaccagcac ttggcaatat tgagcatttt accgccgatg gcggcggata ttgttatttc
     8401 acattctacg gcagcacaac tgtgccagcg tcgtactatt cgttccgtag ggatagcggc
     8461 gtgctaacga taaagcgctc gggccagtgg tatgtaaggt accaggacgc aagcggggag
     8521 ccaatcgccg tgcgagggat agagacgcaa tgggacacaa ccagcggcca catttgactt
     8581 gcgcgtagtc ggaacagacc tcgccggcga cctggagttg gcggccctgt atgatctgag
     8641 ccccggctac ttgtggcttg acttggctgg taacgtgaac tctggttgcc gtgtctgggc
     8701 tccttacagc gttctacgcc acagcacgat accgggcgac ctggcgggtt ccctgactgt
     8761 cagggacgtc ggtgctgctg aacagcaggg cgctttggat cggcggcaac gctacggcca
     8821 atatcgacgt ctggcacgat attgcgcccg tgacgagcgg ctctggtgtg aaaacgggtg
     8881 tgaaaacggg gacgcagctc gtttcttagc gcagagcagg acggggctag gtgttgggcc
     8941 ggattgaccg acagggcagg actgtcatcc taccgcccgg gcggacgagt gttgccaggg
     9001 acgcagtccc tggtagcccg atcggagtac agcatcaatc ctgcgcgttc cacaagtgcg
     9061 gcccaccatg atatccgcgg tcccgagcgc caggcttgcg gcgcgccggc tcgactatca
     9121 agcagcggtc cgccagcatc ctgccgttca gcgctgccgt cgccgcttcc gcctcctcga
     9181 cagtggccat ttcgacaaac ccaaactctt cgctgcggcc gctgtcgcga tccgctacta
     9241 cctgcgcgaa attaacagtt ccgtaatcgc tgaaaagcgc ctgcaactgg tcgctggtca
     9301 tgcccggact caggttgcca acgtaaagtc tatttgacat caggtcacta ggtcgtcagg
     9361 ggaggtcacc aggggaggtc actaagaagc cagaaaggtg actaggtcgt cagcgagttt
     9421 accaatcgcc aaagacaacc gcctcctcgg ccaaacgcca aaatcgccag aggcacccgc
     9481 ctcatcggcc gagcggcgac tcacgtgcgc gataaagctc accttgccac caccaacggc
     9541 cccagccgcc gaaacacgat tatagccgtc gaaattcaca cggctgtagc ttcgcggccc
     9601 ccgcgttgtg cgctggcctc ctccaagttt cgaacctgcc ggacaacaac cagcgctggg
     9661 gccaccagcg gaaccgcccg aacatgcccc caagccgcca ctggcgcgca tacacccaca
     9721 accgcgcact ctggctgaaa cctgacacct gaaaagctgg tggctttata gaatctctag
     9781 caacaattgc gccccgcgtg cagaaaggcg ccactatgcg gcattcgaaa cgtccccact
     9841 gctcacccag cggccttcgt cacctgttcg cctctcacct atcacctctc tccgcgcagc
     9901 ggcgggcctc aggttatcaa aatcgagtac acaa
//
LOCUS       DAOSYS010000134         9783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5907_length_9783_cov_5.458162, whole genome shotgun sequence.
ACCESSION   DAOSYS010000134 DAOSYS010000000
VERSION     DAOSYS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5907_length_9783_cov_5.458162"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLU99_09440"
     CDS             <1..907
                     /locus_tag="PLU99_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO17320.1"
                     /translation="LRRDFTVNGMFFDPLAGQVIDYVGGQPDLKAGVIRAIGEPSARF
                     EEDHLRLLRAVRFAARLNFPIEPRTFAALREHAPQLTKVAAERVRDELERMLSHANRA
                     KAFDLLHQAGLLPFLWPKATWSEQQVAAAKALLARLPAEARFEPAFAVLVLDRPADIH
                     NIARALTFSNDEREDVAWLVEHQRDLDDPASIPLSKLKRLMAHRAFGDLRQLALARWA
                     GDPAAAAWPDALQARLAAIPPEAVQPPPLVTGDDLTARGIPPGPVYKEVLDALYTRQL
                     DLELLTRDAALAALDDLLRERGLLK"
     gene            904..1530
                     /locus_tag="PLU99_09445"
     CDS             904..1530
                     /locus_tag="PLU99_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17321.1"
                     /translation="MTLIKNVGHGGPTLRRVSLACSVMLALGMVGGCAAARMNPAESA
                     ESDPTPRPVTFAIHLAYDEEQPGHQLMISRLGEQVYVAPDVELNRSDLEVARALHSQE
                     RHMLQLVFNYSGRRALEALTRAAVTRANVRPIAPNHAPPVLPGRPASGASPTAKTPPR
                     LAVLINGQLICAPYIDRPVASGEMWIADVFSWQESEDLAAGLNVGHMP"
     gene            1527..2225
                     /locus_tag="PLU99_09450"
     CDS             1527..2225
                     /locus_tag="PLU99_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOO17322.1"
                     /translation="MSSELQITRHPPLVELMLNRPAVRNALSHTLVLELTAELNKAAK
                     DAALRAVIVTGAPPAFCAGLDLNEVAAAGQNVDNAPLLTLFETIENLAKPVIAAVNGP
                     AVAGGAALASVCDVAISAAGAWIAYPGIKRGLVAPIVMSYLCRWVGERRARHLVLTGR
                     AVSASEAVAMGLIDEVVADAEVLERARQIAGDFAKFSAAALRETKRVFGTLRRVAGDP
                     AAVRHLNSTVSRES"
     gene            complement(2279..4081)
                     /locus_tag="PLU99_09455"
     CDS             complement(2279..4081)
                     /locus_tag="PLU99_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOO17323.1"
                     /translation="MTTTTSLFQRVRRELLPYVRQPAQYIGGEVNQLAQPGDWERAAV
                     RVAIAFPDTYAIGMSHLGCQILYTIGNNTPGVCAERVYCPWVDAESIMREKHIPLFTW
                     DTRQPVSEADILAFSLQYEMNFTGVLNLLDLAGIPLQAAARTDEHPLVLAGGPQIDNP
                     EPLADFLDLVVIGDGEPSFAAILAAYGEYKAAGVPRREMIALLARRFPWLYAPNLYEP
                     SYNRDGRLAALMPKFDGLPARIERCRTLDFEQAPVPVRPLVPWIETVHDRIGIEIMRG
                     CPQLCAFCHAGYTKRPLAWRSPQRILEIAEDAWRATGHHEIGLLSLSTADYPKLRELA
                     ESLNSRFAERMVNLSFPSLRIDRMLANIPWLGISVRKGSITIAVEAARDDMRRAIRKK
                     VTDGDLLDGVREVYKAGWNKLKLYFMAGFPGERPDDIDGIFELSKAIADERRRSLGKP
                     PGIVTVSVGWLVPKPFTPLQWAAQPRVEYFHGVRRRLRELSRGGPKYVKLRTHDPERS
                     ILEGVFSRGDRRLCAVVYEAWRRGARFDGWDDTFDDRIWQQAYAATGVDPAWYAHRER
                     PFDELLPWDHIGQHLPRAYLENVYKNLVAALSTP"
     gene            complement(4218..4760)
                     /locus_tag="PLU99_09460"
     CDS             complement(4218..4760)
                     /locus_tag="PLU99_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOO17324.1"
                     /translation="MDVHEAINIRRSVRSYDAKRAIPPEAMERLRAALRLAPSACNFQ
                     PWHFILVTDPKLRHDLSKIANDQHFIADAALIVVACGLPLQAHKRMAGHRNSVEVDVA
                     IALDHLTLAAVAEGLGTCWIGAFDEQKAKRLLEVPEELEIVALMPVGYPASPDLNHPL
                     DESRRKAEGEIFSTDKYGRR"
     gene            5121..5633
                     /locus_tag="PLU99_09465"
     CDS             5121..5633
                     /locus_tag="PLU99_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO17325.1"
                     /translation="MVTWIAPEFNDLEDLFVDQLEDLYDAEKRILDALPKMAQAATSP
                     QVADAFREHLRETENQVRRLDQVFRSIGREPERETCEGIKGIIEEGEKYTKAKGDDMV
                     RDAALICAAQRVEHYEMAGYGTARTFAEQLGYDEAVQLLDQTLQEEKLTDKKLTDLAE
                     QSINVKAAHA"
     gene            complement(5732..6964)
                     /locus_tag="PLU99_09470"
     CDS             complement(5732..6964)
                     /locus_tag="PLU99_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17326.1"
                     /translation="MFWHNRPVPGRIVVVALTTALMLLLSSASADTLRLKNGTVHRGK
                     LVSRDDDKVVFKVERGSITYTIEVRADEVESVEEGQPAPEPTKRAESKSPAAVAVEKL
                     SGKGTPYYVLPLHGPVGTRITRELVSDCLSAARQVGAKVVILDIDSPGGSVSELDSIL
                     RVLDRYSDLRLVAYVHEAKSAAAILAMSCREIIMAERGQIGAAVPYQLAPDGTPKSIA
                     AKFESSLRADFRSVAERGGHNPLLVEGMMRDDIVLALKYDKRGSVRVVESRGDKPLKK
                     RGEILTLTSAEAQEAGLSIGTAADLAGCRSLLRIEEWHEATRHGWQRFRQWAEEIDAA
                     AEKFRQAWQRAQAGLADAEAAAKARRRGEIGDGLRRATANLRQMEELAEKYPGLGDSE
                     SLHALRTRVKAVRQAVDP"
     gene            7208..>9783
                     /gene="ligD"
                     /locus_tag="PLU99_09475"
     CDS             7208..>9783
                     /gene="ligD"
                     /locus_tag="PLU99_09475"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HOO17327.1"
                     /translation="MALTEYRRKRNARRTPEPMPAAGPRPRARRSGRLFVVQKHAATH
                     LHYDFRLELDGVLKSWAVPKGPSLDPADKRLAMHVEDHPLEYASFEGIIPEGEYGGGT
                     VMVWDSGQWEPVGDAAKTYEQGRMKLRLNGQKLRGGWALVRMSGRAADAEGRQWLLIK
                     ERDEYARPRQEFDVTRELPDSVLTGRSLEQIAQQGDQVWESNRAKNTRTRRASAPATA
                     ARKSSTRKSQQTQAAIDPSTLTNARRAAMPERIRPQLATLVKSPPAGDEWRHEIKYDG
                     YRILCFLAGGRAYLFTRHQNNWTAKFPTVADEVGQLPVENAILDGEVVALNPDGTTNF
                     QALQNAFTSGRDRNLVYFVFDLPYCQGYDLSRTPLVERKQLLKTLLGKLRPKARHLRY
                     SDDIQGQGETVFRQACQYKMEGIVSKRSDSTYEQRRTHTWLKIKCLNRQEFVIVGYTE
                     PGGARTAFGALLLAYHDQTGRLIYCGRVGTGFSEAALRRIHGYLKERAQQLPPVTNPP
                     TGAEGRGVHWVRPELVAEVEFGSWTREGILRHPSFLGLREDKRADEVVRENGVGSLAQ
                     DRAESAAARHSAKPSRERQTTKPSRGQSRGNPTAATAVAARADELVIAGVRLTHPDRV
                     LYPEQQVTKAALAEYYTTVADWILPHIVNRPLAVVRCPEGRVKNCFHQKHVSESLPDT
                     IHGVRIREKNQARSEPDAVQITISDLAGLISLVQFGVLEIHPWGARTDNIERPDRLYF
                     DLDPAPEVNWEQVIQAARELRALLDELGLESFVKTSGGKGLHVIVPIARRCDWNQAHA
                     VTRAVAQRLARVAPQRYLATMSKSRRPGKIFIDYFRNARGATTVAPYSTRARPGAPVS
                     TPL"
BASE COUNT         1785 a         3195 c         3126 g         1677 t
ORIGIN      
        1 cctgcggcgc gacttcaccg tcaacggcat gttcttcgat ccgctcgccg gccaggtgat
       61 tgattacgtc ggcgggcagc cggacctgaa ggcgggcgtt atccgtgcca tcggcgaacc
      121 ttcggcgcgc ttcgaagaag atcacctgcg gctgctgcga gcggtgcgat ttgccgcccg
      181 gctcaatttc ccgatcgagc cgcggacctt cgccgcgctg cgcgaacatg cgcctcagtt
      241 gaccaaggtc gcggcggaac gcgtgcggga tgagctggag cgcatgctgt cccatgcgaa
      301 tcgcgcaaaa gcgtttgatc tgctgcacca ggccggcctg ctgccattcc tttggccgaa
      361 ggcgacctgg tcggagcagc aagtggcagc cgccaaagcg ctactggcgc gtttgccggc
      421 ggaggcgcgc tttgagccgg ctttcgcggt gctggttctg gaccggcccg ccgacataca
      481 caacattgcg cgggccctca cgttctcgaa cgacgagcgc gaggacgtag cctggctggt
      541 cgaacatcaa cgcgacctgg acgatcccgc ctcgatcccg ctgtcgaaac tcaagcggct
      601 gatggcgcac cgggccttcg gcgatttgcg gcaattggca ctggcacgct gggcgggcga
      661 ccccgcggcc gcagcgtggc ccgacgcgct gcaagcccgg ctggcggcga ttccgcccga
      721 agccgtccag ccgccgccgc tggtgacggg cgacgacctg acggcgcgcg gcattccgcc
      781 ggggccggtg tacaaggagg ttttggacgc cctgtacacg cggcagttgg atctggaatt
      841 gcttacgcgc gacgcggcgt tggcagcctt ggacgatctg ctccgcgaac gggggttgtt
      901 gaaatgacgc tgatcaagaa cgtcggccac ggggggccga cgctacggcg ggtcagcctg
      961 gcatgcagcg tgatgctggc tcttggcatg gtcggcggct gcgccgccgc cagaatgaac
     1021 ccggctgaat cggccgaatc cgatcccacc ccgaggcccg tgacgttcgc cattcacctg
     1081 gcttacgacg aggagcaacc cggccatcaa ctaatgatca gccggcttgg cgagcaggtc
     1141 tacgtcgcgc cggacgtcga attgaaccgc tccgacttgg aagtcgcacg cgcgctgcac
     1201 agccaggagc gccatatgtt gcaactcgtg ttcaactaca gcggacgtcg ggcgctcgag
     1261 gcgttgacgc gcgccgccgt gacgcgcgcc aacgttcgcc cgatagcgcc caatcacgct
     1321 ccccccgtgc tacccgggcg tcccgcgtct ggcgcatcac caaccgcgaa aacgccgccg
     1381 cgcctggccg tgctgattaa tggccaactg atctgcgcgc cgtacatcga tcgtcccgtt
     1441 gccagcggcg agatgtggat cgccgacgtg ttctcctggc aggagagcga agacctggcc
     1501 gccggactta acgtggggca catgccatga gcagtgaact ccaaatcacc cgccacccgc
     1561 cgctcgttga actgatgctc aaccggccgg ccgtgcgcaa cgccctctcg cacacgcttg
     1621 tcctcgagct gacggccgag ctgaacaaag cggcgaaaga cgccgcgctc cgcgccgtta
     1681 tcgtcacggg cgcgccgccg gcattctgcg ccgggctgga cctcaacgaa gtcgcagcgg
     1741 ccgggcagaa cgtcgacaac gcgcccctgc tgacgttatt cgaaaccatc gagaacttgg
     1801 cgaagccggt gatcgccgca gtgaacgggc cggccgtggc gggcggtgcg gcgctggcct
     1861 cggtttgtga cgtggcgatc tccgcggctg gggcgtggat tgcatatccc ggcataaagc
     1921 gtggcctggt cgcgccgatt gtcatgagct atctgtgccg ctgggtgggc gagcggcgag
     1981 cgcgtcacct ggtcctgacc gggcgtgccg tctcggccag cgaggccgtc gcaatggggc
     2041 tgatagacga ggtcgtcgcc gacgctgaag tacttgagcg ggcgcggcag attgccggcg
     2101 acttcgcgaa attttcagcg gcggcgctgc gcgagacgaa gcgggtattc ggcacgttgc
     2161 gccgggtggc tggtgatccg gcggccgttc ggcatctcaa ttcaacagtc tcgcgtgagt
     2221 catgatccgc aaaagtgcgt cgacggcgcg cggttaacca acgcgccgaa gacacaccct
     2281 acggcgtcga gagagcggcg accagattct tataaacgtt ttcgaggtac gcgcgcggca
     2341 ggtgctggcc gatgtggtcc cagggcagca gttcgtcgaa gggccgctcg cggtgggcat
     2401 accaggccgg atcgactccg gtggcggcgt aggcttgctg ccagatgcgg tcgtcaaacg
     2461 tgtcgtccca gccgtcgaag cgcgccccgc gccgccaggc ttcgtaaacc acggcgcaca
     2521 gacggcggtc gccgcgcgag aagacccctt ccagaatcga acgctccgga tcgtgcgtgc
     2581 gaagcttcac gtacttcggc ccgccgcggc tcaactcgcg cagccggcgc cgcaccccgt
     2641 ggaagtactc gacgcgcggc tgagccgccc attgcagcgg cgtgaacggc ttgggcacca
     2701 gccagccaac cgacaccgtg acaatgcccg gcggtttgcc gaggctgcgg cgccgctcgt
     2761 ccgcgatggc tttgctcagc tcgaaaattc cgtcgatgtc gtccggccgc tcgccgggaa
     2821 agccggccat gaaatagagc ttgagcttgt tccagccggc cttgtagacc tcgcgcacgc
     2881 cgtccagcag gtcgccatcg gtgacttttt tgcgaattgc gcggcgcatg tcgtcgcggg
     2941 ccgcctcaac cgcaatcgtg atgctaccct tgcggaccga gatgcccagc cagggaatgt
     3001 tggcgagcat gcggtcgatg cgcaacgatg ggaagctcag gttgaccatg cgctcggcga
     3061 agcgcgagtt gagcgactcg gccaattcgc gcaacttcgg gtaatccgcc gtagataacg
     3121 acaataggcc gatttcatgg tgcccggtgg cgcgccaggc gtcttcggcg atctcgagga
     3181 tgcgctgcgg cgaacgccag gccagcgggc gcttcgtgta tcccgcgtgg cagaaggcgc
     3241 acagttgcgg gcagccgcgc atgatctcga tgccgatgcg atcgtggacc gtttcaatcc
     3301 agggcaccag cgggcggacg ggtaccgggg cctgctcgaa atccagcgtg cggcagcgct
     3361 cgatgcgtgc gggcaggccg tcgaacttgg gcatgagcgc ggcgagcctg ccgtcgcggt
     3421 tgtaactcgg ctcgtacagg ttcggcgcgt agagccaggg aaagcgccgg gccagcagcg
     3481 cgatcatctc gcgccgcggc acgccggccg ccttgtattc accgtaggcg gcgaggatcg
     3541 cggcgaaact cggctcaccg tcgccgataa ccaccaggtc gagaaaatcc gcgagcggct
     3601 cggggttgtc gatctgcggt ccgccagcca gcaccagcgg gtgctcatcc gtgcgagccg
     3661 cagcttgcag agggattccg gccaaatcca gcaagttcag cacgccggtg aagttcatct
     3721 cgtattgcag cgagaaggcg aggatgtcgg cctcgctgac cggctgccgc gtgtcccagg
     3781 tgaacagcgg gatgtgcttc tcgcgcatga tgctttcggc gtcgacccag ggacagtaaa
     3841 cgcgctcggc gcaaacgccg ggcgtgttgt tgccgatcgt gtacaggatt tggcagccca
     3901 ggtgcgacat gccgatcgcg tacgtatccg ggaacgcgat cgcaacccgc accgccgccc
     3961 gctcccaatc gcccggttgc gcaagctgat tgacctcccc gccgatgtac tgggcgggct
     4021 gccgtacgta gggcaacagc tcccggcgca cgcgctgaaa aagtgaggtg gtcgttgtca
     4081 ttccttcagc attcgcgggc agtccggtcc cgattgaccg cgcaacggcg agccaacgcc
     4141 gagtataggg gcccgtggcg cgctacgccg cccggaggca ttcgcggagg ccacttgcag
     4201 cggctcattc cctgagctta tcgccgcccg tacttgtccg tgctgaaaat ctcgccctca
     4261 gctttgcgcc gcgactcgtc cagcggatgg ttcagatccg gcgacgccgg gtagccgact
     4321 ggcatgagcg ccacaatctc caactcctcc ggcacttcca ataggcgctt ggccttctgt
     4381 tcgtcaaagg cgccgatcca gcaggtgccc agcccctcgg cgacggccgc cagcgtcagg
     4441 tgatcgagcg cgatcgccac gtcgacctcg acgctattgc ggtggccggc catccgcttg
     4501 tgagcctgca gcggcaggcc gcaggcaacg acgatcagcg ccgcgtccgc gatgaagtgc
     4561 tgatcgttcg cgatcttgct caagtcgtgc cgcagcttgg gatcggtaac cagaatgaag
     4621 tgccagggct gaaaattgca ggccgacggg gctaaacgca gggcggcgcg caaacgctcc
     4681 atcgcctcgg gcgggatggc gcgcttggcg tcgtagctgc gaacgctgcg ccggatatta
     4741 atggcctcgt gcacgtccat gctcatcctc cagggtgacg aacagtatat cgcagaacga
     4801 gagggatcag ggatctaggg atgaaaggga ttaagaggtg tgaagagcag agccggggcg
     4861 taccgccccg gattgccgcc aacggcctcc catccgcgta gtagcccgcg tcgcctctgc
     4921 cgtttcccga tccgaggccg cccgatccga gcccgaagcg cgagcgaggg caaccggctc
     4981 ccgaagcgca agtgggcttt caccgacttc tcaggtggta ttcatcgtgt tttcaggccg
     5041 ccgcgctacc atcctcggac ctttgggcgc tgagagagtc ttcaggcgcc gatcatgttc
     5101 agaccgaaag gagaattcgc gtggttacct ggatagcccc cgagttcaac gatctcgaag
     5161 atctgtttgt cgatcaactg gaagaccttt acgacgcgga aaaacgcatc ttggatgccc
     5221 tccccaagat ggcccaggcg gccacctcgc ctcaggtggc tgatgctttc cgtgagcatc
     5281 tgcgggaaac cgaaaaccag gtgcgccgat tggatcaggt gttccgtagc attggccgcg
     5341 agcccgaacg cgagacctgc gagggcatta agggcattat tgaggagggt gagaagtaca
     5401 ccaaggccaa gggcgacgac atggtgcgcg acgcggcgct catctgcgcc gcccagcgcg
     5461 tcgagcacta tgaaatggcc ggctacggaa cagcccgcac ctttgccgag cagcttggct
     5521 atgacgaggc agtacaattg ctcgaccaga cattgcaaga ggaaaagctt accgacaaga
     5581 agctgaccga tctggccgag caatcgatca acgtcaaggc cgcgcacgca taaggcgctc
     5641 ctggcgtgtc atagtggctg gatactagtg agagcgcgcg gcccagtccc gtggcgcgcg
     5701 ctttcctttt ctttcgccta tgcggcgcaa tttagggatc gaccgcctgc cgcaccgctt
     5761 ttacccgcgt gcggagcgcg tgcagcgact cgctgtcgcc caggcccggg tacttctcgg
     5821 ccagctcttc catttgccgt agattggcgg tggcgcggcg caggccgtcg ccgatctcac
     5881 cccgccggcg cgccttggcg gccgcctcag catctgcgag cccagcttgc gcccgctgcc
     5941 aggcctggcg gaatttttcg gccgccgcat cgatctcctc ggcccattgc ctgaagcgct
     6001 gccagccgtg gcgtgtggct tcgtgccact cctcaatgcg caacagactg cggcagccgg
     6061 ccaagtcggc cgcagttccg attgacagtc ccgcctcttg cgcctcggcc gaggtcagtg
     6121 tcagaatctc gccccgcttc ttcaacggct tgtcacccct gctctcgacc acacgcaccg
     6181 agccgcgttt gtcgtacttg agcgccagca cgatgtcatc ccgcatcatg ccctcgacca
     6241 gcagcggatt gtgccccccg cgctcggcca cggaccggaa atcagcccgt aaagacgact
     6301 caaacttggc cgcgatgctc ttgggcgtgc cgtccggggc aagttggtag gggacggcgg
     6361 ccccgatctg cccgcgctcg gccataatga tctcgcggca cgacatggcc aggatggcgg
     6421 cggcggattt ggcctcgtgc acataggcca ccaggcgcaa gtcgctgtag cggtcaagca
     6481 ctctcagaat gctgtcaagt tcggagaccg atccgccggg actgtcaatg tcgaggatga
     6541 cgacctttgc gcccacctgc cgtgcggcac tgaggcaatc gctcaccaat tcgcgcgtaa
     6601 tgcgtgtacc aacgggcccg tgcagcggca gcacgtagta gggcgtaccc ttcccgctca
     6661 acttctcgac tgcaaccgcg gccgggctct tgctttcagc ccgcttcgtc ggttcagggg
     6721 ccggctggcc ttcttcaacc gactccacct cgtcggcccg aacctcgatc gtataagtaa
     6781 ttgagcccct ttcgactttg aagaccacct tgtcatcgtc tcgggaaacc agcttgcccc
     6841 ggtgcacggt cccgttcttg agccgcagtg tgtcggcgct agctgaactg agcagcaaca
     6901 ttagcgcggt cgttaacgcc acaaccacaa tccgcccagg gacaggccga ttatgccaga
     6961 acatatttgc tcctccggct ttgaaaccca cgcgcccgaa cgggcaccga gcgacgctaa
     7021 aacccggtct ttgagttgcc agcataccat ttgttaagcg cggtcggcaa gtaaacctta
     7081 cggccggtaa atgtgaactg gcaggcgcct attgctactc tcatttgcgg agcccagtaa
     7141 aagccggtcc cggcggctgg gcgaggccgc ccgcgccgct tattgtgtag ctacgaggta
     7201 tgttcccgtg gcattgaccg agtaccgccg caaacggaat gcccgccgta cccctgagcc
     7261 catgcccgcg gccggccccc ggcctcgcgc gcgccgcagc ggccgcctct tcgtggtgca
     7321 gaagcacgcc gccacccacc tgcactacga tttccgcctg gagctggacg gcgtactcaa
     7381 gagctgggcc gtgcccaagg gcccctcgct cgacccggcc gacaaacgcc tggcaatgca
     7441 tgtcgaggat cacccgctgg agtacgcctc tttcgagggc attatccccg agggcgaata
     7501 cggcggcggc acggtcatgg tctgggacag cgggcaatgg gagccggtcg gcgacgccgc
     7561 caagacttac gagcagggcc gaatgaaatt gcgcttgaac ggccagaagc tgcgcggcgg
     7621 ctgggcgctg gtgcgcatga gcgggcgcgc ggcggatgcc gagggccggc aatggctgtt
     7681 gatcaaggag cgcgacgaat atgcccgccc gcgccaagaa ttcgacgtga cgcgtgaact
     7741 gccggacagc gtcctcacgg gccgcagcct cgaacagatt gcgcaacaag gcgaccaggt
     7801 ctgggaatcg aaccgcgcga agaacaccag aaccaggcgc gcttcggcgc ccgcaactgc
     7861 cgcccgcaag agcagtactc gcaagtcgca gcagacccag gcggcaatag acccgtccac
     7921 actcaccaat gcccgccggg ccgctatgcc ggaacgcatt cgtccccaat tggcgacact
     7981 cgtaaagtcg ccgccggccg gggatgagtg gcggcacgaa atcaagtacg acggctaccg
     8041 catcctatgt tttctggccg gtggacgcgc ctacctcttt acccggcatc agaacaactg
     8101 gactgcgaaa ttcccaaccg tggcggacga ggtgggccag ctccccgtgg agaacgccat
     8161 tctggacggc gaagtggtcg ccctcaatcc tgacggcaca accaacttcc aggcgctgca
     8221 gaatgcgttc acgagcggcc gcgaccgaaa cctggtctac tttgtctttg acttacctta
     8281 ctgccaggga tatgacctct cgcggacgcc gctggtcgaa cgcaagcagt tgctaaaaac
     8341 tcttctggga aagctaaggc cgaaagcacg ccacttgcgc tacagcgacg atattcaagg
     8401 ccagggcgag accgtgttcc gccaggcctg ccaatacaaa atggagggca ttgtttccaa
     8461 gcgcagtgac agtacctatg agcagcgccg cacacacacc tggctcaaga taaagtgcct
     8521 caatcgccaa gagttcgtga ttgtcggcta caccgaacct ggcggcgcgc gaacggcttt
     8581 cggcgcgctg ctgctcgcgt accatgacca aaccggccgg ctgatctact gtgggcgcgt
     8641 cggaaccggc tttagcgaag ctgccttacg gcgtattcat ggctacctga aagaacgcgc
     8701 ccagcagcta ccgccggtca cgaatcctcc gaccggcgcg gagggccgcg gggtacattg
     8761 ggtccgcccc gagttggtgg ccgaagttga attcggctcg tggacgcgcg aaggcattct
     8821 tcgacatccg tcattcctgg gcttgcgtga ggacaaacgc gccgatgagg tcgtgcgcga
     8881 gaacggcgtc gggagtcttg cccaagaccg tgcagagagc gccgccgccc gccacagtgc
     8941 gaagccgtcg cgcgaacgcc aaactacgaa gccgtcgcgt ggacaaagcc gcggtaaccc
     9001 cacagcagcg acggctgtcg cagcgcgcgc tgacgaactg gttatcgccg gcgtgcgcct
     9061 cacacacccg gatcgcgtgc tctaccctga gcagcaagtg accaaagccg cgctcgccga
     9121 atactacacc acagtagcgg attggatact tccgcacatt gtgaaccggc ctctggcggt
     9181 cgtgcgctgt cccgaaggcc gcgtgaagaa ctgcttccac cagaagcacg tcagcgaatc
     9241 gttaccggac acgatccacg gcgtgcggat ccgcgagaag aaccaggcgc gcagcgagcc
     9301 ggacgccgtg cagatcacca tctccgactt ggccggattg atcagcctgg tgcagttcgg
     9361 cgtgctcgag attcatccct ggggagcgcg cacggataac atcgagcggc ccgaccgctt
     9421 gtacttcgac ttggacccgg cccccgaggt gaattgggag caggtcatcc aggcggcccg
     9481 cgagctgcgc gcgttgttgg acgaattggg gctggagagc tttgtaaaga cttccggcgg
     9541 caagggcctg cacgtaatcg tgccgattgc gcggcgctgc gactggaacc aggctcacgc
     9601 ggtcactcgg gcggtggcgc agcgcctggc gcgggtcgcg ccgcagcgat atttggcgac
     9661 catgagcaag tcacgccggc cgggcaaaat attcattgat tactttcgca acgcgcgcgg
     9721 cgcgacgacc gttgcgccct attcaacccg ggcccgcccc ggcgcgccgg tttccactcc
     9781 gct
//
LOCUS       DAOSYS010000135         9767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5930_length_9767_cov_5.504633, whole genome shotgun sequence.
ACCESSION   DAOSYS010000135 DAOSYS010000000
VERSION     DAOSYS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5930_length_9767_cov_5.504633"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLU99_09480"
     CDS             <1..384
                     /locus_tag="PLU99_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOO17328.1"
                     /translation="EATLDKMDRDDPAYVKKMSEVLESRISHKTWFEFQQANMTREVA
                     LVTDRIYRDILAATQSIAENAGYDLVLYRDEYQPVSDPDDVQSQMRSRKVIYARDTVD
                     ITEAVLGKLNADFRSRPRVPDLQMP"
     gene            445..1503
                     /gene="lpxD"
                     /locus_tag="PLU99_09485"
     CDS             445..1503
                     /gene="lpxD"
                     /locus_tag="PLU99_09485"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOO17329.1"
                     /translation="MAGEIAITVGELAQRVGGDVHGDAGLVIRGIGTLEEAGPHMLSW
                     IASKQLLPRLAQSAAGAVLMPLDCEAPAGRTAIRVRDPELVLCDLLSWLGPPVDVVPA
                     GVHESAVVAPDAQVEGAAIGPLVTIGARSRIGPRTQLHAGVRIGSDVVIGSDCVLWPN
                     VVVRERVTIGERVIIHPHATIGADGFGYLQRGGQHRKIPQMGTVVIEDDVEIGAASCI
                     DRARTGATCIGRGTKIDNLVQVGHNVVVGEGCIIVAQCGISGSTVLGRHVVFGGQVGV
                     IDHMRVGDRVQVAAKSAVMTDLAADAIYRGIPARAQWQFGRQEIGVRRLPKMIEQVRE
                     LMRRVERLESAADDRAGS"
     gene            1469..2779
                     /gene="lpxC"
                     /locus_tag="PLU99_09490"
     CDS             1469..2779
                     /gene="lpxC"
                     /locus_tag="PLU99_09490"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOO17330.1"
                     /translation="MNQQQTIEREVELTGRGLFTGFPVRVRFKPAPVGTGITFVRTDG
                     PDPVRVAARIDNLSKRARRTSLKNGTVTIETVEHCLAALRALAIDNVEVELDNAELPS
                     GDGSCLLFTESLISAGAVPQEADRKYLHVLHPARVSDGDSELVAWPGSGEQLDIYYEL
                     DYGHENPVGRQTCHFVLDRDRFLTEIAPSRTFVLEEEARQLREAGLGQHLTYQDVLVI
                     GADGPIENSYRFADECVRHKVLDLIGDLMLSGGFVCGRIFARRSGHHLNHELVRRILE
                     QHANQAAQETLSRAPRLDIEAIQRILPHRYPMLLVDRVIEVEGDRRAIGVKNVSINEP
                     FFQGHYPRKPIMPGVLIIEAMAQLGGLLLSQKLEHTGKVAVLLSLEGVKFRRPVVPGD
                     QLMLEAQAIRVKSRSGEVFCQARVASELAAEANIRFMLVDADPS"
     gene            2789..3697
                     /gene="lpxA"
                     /locus_tag="PLU99_09495"
     CDS             2789..3697
                     /gene="lpxA"
                     /locus_tag="PLU99_09495"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOO17331.1"
                     /translation="MTQIHPTAVVDPQATLGQEVKIGPYCVVGAGVVLGDGCELMAHV
                     TLLGPSEFGACNLFYPHCVLGGAPQDLKYKGGPTRLVVGSHNAFREHVTAHRGTEIDR
                     LSGGVTRIGDHNLLMVGVHVAHDSHLGNHLLIANNVQIAGHVRIEDRVVVGGVAAMHH
                     FVTIGRDAYVAGMTRVVHDVPPYMKVMGHAQTVRGVNTEGMRRWQIPEESIRNLRTAA
                     RLLYARRRSQLPLCTAAALNEIESNGLMRDEHVAYLVAFVRRMLTAGKFGRVREHERS
                     DLPADRQWFYQAGTAGGADSASGWKT"
     gene            3747..4805
                     /locus_tag="PLU99_09500"
     CDS             3747..4805
                     /locus_tag="PLU99_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO17332.1"
                     /translation="MSETIPVAVMGAGHMGRYHAQRYAQMAGVRVAAIVDTNVERARA
                     LAEPLGARWAAAFCPELGDIVAASVAVPTVHHLAVARPLIEAGVAVLIEKPLAPNVAD
                     AEQIAELAARHNVLVQVGHTERFNPAVQAVERLGVQPKFIETHRISPFTFRSADIGVV
                     FDMMIHDIDILLHLMRDEVVRVDAVGVNVLGPHEDIANARVSFRRGAVANLTASRLAL
                     KTERKLRVFSETAYVSLDYQKKAGIAVTRDRNLDVLQMARERNMQDLSQMAGLDFGKL
                     VHIEPLMIDPRDPLEAELEAFITCVRNRKTPPVSAADGVAAVRLAAQIVESLSAHNWD
                     GPDGQRIGLSADIFGSSH"
     gene            5155..6891
                     /locus_tag="PLU99_09505"
     CDS             5155..6891
                     /locus_tag="PLU99_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17333.1"
                     /translation="MAIIFTGFAAAYLIARNAWEEQTMAARQAEAARLATLTADAVGS
                     GMQSGMAAALLRQFAQRPGVRAIQWTGPAGEVRFAWPLADQAVPPLAATEVEDLTVAA
                     ANVRTPEGVLEGVVRIELAGAWDFASDRHLFWNWAIAACTTLIVFVLLYRRLRGHLRP
                     LAALHQNLEHYAAGIERQLEALTLGDALGATAQSWNMLIREFAAARQQLETVRRANQT
                     GDILTRFEGAVFRRMVDRLPFGVLCVNSDQTVTYANGSALALLGARSEAVLGQPLGEA
                     VGAPAVAAAVSGAQARSSAGLAIDHTLTTGESETTLRFRVLPLSDEAAGGETLVSIED
                     IGQLKESERARDNFLYHVTHELRTPLTSIHAYAETLTRPGFDDEQTRKECYNVIMSET
                     RRLSSLVEDILSLSQLEVGTARLDVGDVDLVRLIRQMVQDNLGAADEKRIDLTLTLPP
                     KTPKVRGDKQRLSVLLMNLIGNAIKYTPENGTVQVTLTASEQRVAVSVRDTGIGIAPE
                     DQAHVFEKFYRASNDKVQAVAGTGLGLAIAREVARLHGGDIHLQSEPGQGSTFTVELP
                     VSNTESAEAFAR"
     gene            6888..7343
                     /locus_tag="PLU99_09510"
     CDS             6888..7343
                     /locus_tag="PLU99_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17334.1"
                     /translation="MSRILVAEDDPHILRLIAMWLRRQGHEVLEARHGAAALALIEAQ
                     AVDVLVSDVNMPGVDGLELIEAILPQSRVRRGVIVLTNRWDHGEIRERLARWGVQVMP
                     KPFSPTRLSELVEALVRADGGPAKSEENLDLAAGATEGQFAPPASLPPA"
     gene            7359..8591
                     /locus_tag="PLU99_09515"
     CDS             7359..8591
                     /locus_tag="PLU99_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOO17335.1"
                     /translation="MFGSDANTLWFAAGAALAAVAVLAGFAFTRRRDSLAEPAPPAAR
                     GFSAAALDLGRRPAGVADAMCRSFLEWLAEHEDQAELWASFDQLVRELLTEHLGANRV
                     RCYHVRAGAEALQPLSGGHGEMVGASSRHGVLGHVATSGKEFVAGDPGCGPLLDDLAA
                     RTEEPWVWIWPVREAGGTLGVVAVGNLRQVGAPGFGLRRTVGQLLTIFWRHTACVERW
                     RVVQRTDRGSGVLTRNDFFTLAAHALNDSYQTNEPVVAVVLALEGMRRLDDSGRWEER
                     DRLIERLGCALARRVRSDDLVGRFADDRFVVLLRRLDSGLGRLIAEKMLAAGEECLEQ
                     MAGGRENLRLRLGLAGSGHGQPPLEKLLVTAFAAAERARRANVPIATDLLEAGGRGGA
                     AAGESALEPQAKTEVSST"
     gene            8588..8923
                     /locus_tag="PLU99_09520"
     CDS             8588..8923
                     /locus_tag="PLU99_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO17336.1"
                     /translation="MKVQTQQQGNVTVVGLHGPLITDELEDVRRALQTGNGDLRRTIL
                     DMSDVPYLDSNGIELLLELCGAHLSPHQRPKMAALSETCLEALELTDVLPRLDVFDTV
                     ENAIRSCQR"
     gene            9001..>9767
                     /locus_tag="PLU99_09525"
     CDS             9001..>9767
                     /locus_tag="PLU99_09525"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO17337.1"
                     /translation="MRTNKLGQLLVEQGLVSEADLLRALEAQNKDNKRLGEVLIDMGI
                     LSPKAMLSALAKQLKVKCCHLRHGLIDPAVARLVDREQAARLRVLPLFKVGNTLTVAM
                     AEPQSLPSVDLLSAVTGCQINPVLALESNIAEFQEKYLGEEVDIDSFLVNLTESEVKV
                     VERETVDEDTPQEIGKMVAGSPIINLVNLAIMTAIRDGASDIHVEPTKRNTCIRYRID
                     GNLRELMTAPNTMHAAIVSRIKVIGKMDIAERRLPQEG"
BASE COUNT         1734 a         2712 c         3625 g         1696 t
ORIGIN      
        1 gaggccacgt tggacaagat ggatcgcgat gacccggcgt acgtgaagaa gatgtccgag
       61 gtgctcgagt cgcggatcag ccacaagacg tggtttgagt ttcagcaggc gaacatgacg
      121 cgcgaagtag cgctggtcac ggaccgcatt tatcgcgaca ttctggcggc cacgcagagc
      181 atcgccgaga atgccggata tgacctggtg ctttatcgtg atgagtatca gccggtcagc
      241 gacccggacg acgttcagtc gcagatgcga tcgcgcaagg tgatctacgc ccgggacacc
      301 gttgatatca cggaggcggt tttgggcaaa ctgaacgctg acttccggtc caggccgcgc
      361 gtgccggatt tgcagatgcc gtgagtccaa gggatcgaag gaaggaagga tcaaggccgg
      421 gggtagcgag gcgggctgag gcagatggcg ggcgagatcg cgatcaccgt tggcgaactg
      481 gcgcagcgcg ttgggggcga tgtgcacggc gacgccgggc tggtcatccg cggcattggc
      541 acgctggaag aggccgggcc gcacatgctc tcatggatcg cctccaagca attgctgccg
      601 cgcttggcgc agtcggccgc gggcgcggtg ctgatgcctt tggattgcga ggcgccggcc
      661 gggcgcaccg cgatccgcgt gcgcgatccg gagcttgtgc tatgcgacct gctgagctgg
      721 cttgggccgc cggtcgacgt cgtgccggcc ggcgttcacg agagcgcggt tgtcgcgcct
      781 gatgcacagg ttgaaggcgc ggcaatcggg ccgttggtga cgatcggagc gcgcagccgc
      841 atcgggccgc ggacgcaact gcacgcgggc gtgcggattg ggtcggacgt ggtcattgga
      901 tccgattgcg tcttatggcc gaacgtggtg gtgcgcgaac gtgtgacgat cggcgagcgc
      961 gtgattattc acccccatgc cacgatcggc gcggacggtt ttggttatct gcagcgtggc
     1021 ggccagcacc gtaagattcc ccagatgggg acggtggtca ttgaggatga tgtcgagatt
     1081 ggggcggcga gctgcataga cagggcgcga accggggcca cgtgcatcgg ccgggggacg
     1141 aagattgata acctggttca ggtgggacat aatgtggtgg tcggcgaagg ctgcataatt
     1201 gtcgcccagt gcgggatttc tggatcgacc gtgctggggc ggcacgttgt gttcggcggc
     1261 caggtgggtg tgatcgacca catgcgcgtc ggagaccgcg tgcaggtggc ggccaagtcg
     1321 gccgtcatga ctgatctggc ggcggatgcc atttaccgcg gtattcccgc gcgggcgcag
     1381 tggcagttcg ggcggcagga gatcggcgtg cggcgcctcc caaagatgat cgaacaggtg
     1441 cgggagctga tgcgacgagt ggaacggctt gaatcagcag cagacgatag agcgggaagt
     1501 tgagctgact gggcgcggcc tgttcaccgg gttcccggtg cgcgtgcgct tcaagccggc
     1561 cccggtgggg acggggatta cttttgttcg cacggacggg cccgatccgg tgcgcgttgc
     1621 ggcgcgtatc gacaatttat cgaagcgcgc ccgccgcacg tcgctcaaaa acgggactgt
     1681 cacgatcgaa actgtcgagc actgcctggc cgctttgcgg gccttggcga tcgacaatgt
     1741 tgaggttgag ctggataacg ccgagctgcc ttcgggcgat ggcagttgcc tgctgttcac
     1801 cgaatcgctg atctcggccg gggccgtacc gcaggaggcg gaccgcaagt acctgcacgt
     1861 gctgcatccg gcgcgggtca gcgacggaga ctcagaactg gtggcctggc cgggcagcgg
     1921 cgagcagctc gacatttact acgagttgga ctacgggcac gagaacccgg tcgggcggca
     1981 gacttgccat ttcgtgcttg accgcgatcg cttcctgacg gagatcgctc cgtcgcggac
     2041 cttcgtgctg gaggaagagg cgcgccaact acgggaggcc gggctggggc agcatctaac
     2101 gtaccaggac gtgctggtaa tcggagcaga tgggccgatc gagaacagtt accgcttcgc
     2161 cgacgagtgc gtgcggcaca aagtgctgga tttgatcggc gatctgatgc tgtcgggcgg
     2221 gtttgtttgc gggcgtatct tcgcgcggcg ctcggggcac cacctgaatc acgaactggt
     2281 gcggcgcatc ctggagcagc acgccaacca ggcggcgcag gagacgctca gccgagcccc
     2341 gcggctcgac attgaagcca tccagcgcat cctgccgcac cgctatccga tgctgctggt
     2401 ggaccgcgtg attgaagtag aaggtgatcg ccgggccatc ggcgtaaaga acgtgagcat
     2461 aaatgagccc ttcttccagg ggcattaccc gcgcaagccg atcatgccgg gggtgctgat
     2521 tatcgaggcc atggctcagc taggtggcct gctactatcg cagaaactcg agcacacggg
     2581 aaaagtggcc gtgctgctaa gtttggaggg agtgaagttt cgccggccgg tggtcccggg
     2641 tgaccagttg atgctggaag cgcaggccat ccgggtgaaa tcgaggagcg gggaggtttt
     2701 ctgccaagcg cgggtggcgt cggaactggc ggcggaggcg aacatccgct tcatgctggt
     2761 cgacgcggac ccgagctaga cgtgagttat gacccaaatc catccgaccg cggtagttga
     2821 ccctcaagcg acactcgggc aggaagtcaa gattggaccg tactgcgtgg ttggggcggg
     2881 cgtcgtactg ggcgacgggt gcgagttaat ggcgcacgtg acgttgctgg ggccgtcgga
     2941 attcggcgcc tgcaaccttt tttatccgca ctgcgtgctg ggcggcgcgc cgcaggatct
     3001 taagtacaag ggcggcccta cacgtctggt ggtgggcagc cacaatgctt ttcgcgagca
     3061 cgtcactgcc catcgcggga cggagatcga ccgcctcagc gggggcgtga cgcgcatcgg
     3121 cgatcacaac ctgctgatgg tcggggtaca cgtggcgcac gacagccacc tcggcaatca
     3181 cttgctgatt gccaacaacg tgcagatcgc ggggcacgtg cgcattgagg atcgcgtcgt
     3241 ggtgggcggg gtggcagcga tgcaccactt tgtgacgatc gggcgcgatg cgtacgtggc
     3301 cggcatgacg cgcgtcgtgc acgacgttcc accgtacatg aaggtgatgg ggcacgcgca
     3361 gaccgtacgc ggggtgaaca ccgagggcat gcggcgctgg cagattccgg aagaatcgat
     3421 tcgaaacctt cgcacggccg cccggctgct gtatgcgcgg cgccgcagcc aattgccgct
     3481 atgcacggcg gccgccctga acgagatcga aagcaacggc ctgatgcgcg acgaacacgt
     3541 ggcctatctg gttgcgttcg tgcggcgcat gctgaccgcg ggcaagttcg ggcgcgtgcg
     3601 ggagcatgag cgcagcgatc tgccggcgga tcgacagtgg ttctaccaag ccggaacagc
     3661 cggcggggct gacagcgcga gcggttggaa aacctagcga ttgaaaaccg gtttcacgcc
     3721 gtggaaccgg cgagcggtga acaagcatga gcgagacgat tccagtagca gtgatgggtg
     3781 cggggcacat gggtcgctac cacgcccaga gatatgcgca gatggccggg gtccgtgtgg
     3841 cggcgatcgt cgacacgaac gtggaacggg cccgcgcgtt ggcggagccg ctcggggcgc
     3901 gttgggcagc ggcattttgt ccggaactag gcgatattgt ggccgccagc gtggccgttc
     3961 cgacggtaca ccatttggcg gtggcgcggc cgctgatcga ggcgggcgtg gcggtgctga
     4021 ttgaaaagcc gctggcgccg aacgtggcgg acgccgagca gatcgcggaa ttggccgcgc
     4081 ggcacaatgt tctggttcag gtggggcaca cggaacgctt taatccggcg gtgcaggcag
     4141 tcgagcgtct gggcgttcag ccgaagttca ttgagacgca tcggatcagt ccgttcacgt
     4201 ttcgttcggc ggatatcggc gtcgtgttcg acatgatgat tcacgacatc gacattttgc
     4261 tgcacctgat gcgagacgag gtggtgcgcg tggacgcggt gggcgtgaac gtgctggggc
     4321 cgcacgagga catcgccaac gcgcgcgtca gctttcggcg cggggcggtc gcgaacctca
     4381 cggcgtcgcg tttggcgctg aagaccgagc gcaagctgcg ggttttcagc gaaacggctt
     4441 acgtttcgct cgattaccag aagaaggcgg gcatcgcggt gacgcgcgac cggaatctcg
     4501 acgtgctgca aatggcgcgt gagcggaaca tgcaagacct gtcgcagatg gcgggactgg
     4561 atttcggcaa gctggtacat attgaaccgc tgatgatcga tccgcgcgat ccgctggaag
     4621 cggagctgga agcattcatc acatgcgtac ggaatcgcaa gacgccgccg gtttcggccg
     4681 cggacggcgt agcggcggtt cgcctggcgg cgcaaatcgt agagtcgctg agcgcccata
     4741 attgggacgg cccggacgga cagcgaatag ggctgtcggc cgatattttc ggctcgagcc
     4801 actgacaatt ccggggaatc ccgccttcgt ggcggccggt tgaatccggc tcggcatatc
     4861 ttcacctttc cgcgcgcggg ctcgccgtcg gccgcagtgc atcaattatg ggcgggggcg
     4921 tgtagatatc ggcgcgcatc ggccgatggc gcggattgca gttatcggtg gacccgcctc
     4981 tcttcaagtc ctcaatgggc agcaccgatg cgccggtgaa gactcgaagc gccgagggag
     5041 aacaggccgg ccgtgtgcgc agaacaagta caggaaatca cgcctaagca accggcgagc
     5101 gtgcgccatt tagtgtggcg gggcgtttta ccggaagcgg tggggttatg ctttgtggcg
     5161 atcattttta ccgggttcgc ggctgcctat ctgatcgccc gcaacgcctg ggaggagcag
     5221 acgatggcgg cgcggcaggc ggaggcggcg cgactggcta ccctgaccgc cgacgccgtc
     5281 ggcagcggaa tgcagtcggg catggcggcc gcactgctgc ggcaattcgc ccagcgtccg
     5341 ggcgtgcgcg cgattcaatg gacgggaccg gccggcgagg tgcgattcgc gtggccattg
     5401 gcggaccagg ccgtgccacc gctggcggcg accgaggttg aggacttgac ggtcgcggcg
     5461 gcgaacgttc ggactccgga aggagttctt gaaggcgtcg tgcggatcga attagcgggg
     5521 gcgtgggatt tcgcgagcga ccggcacctg ttctggaatt gggccattgc ggcgtgcacg
     5581 acgctgatcg tcttcgtact cctgtatcgg cgtttgcggg gtcacctgcg accgctggcg
     5641 gcgttgcatc agaatctgga gcactacgcc gcgggcatcg agcggcagtt ggaggcgctg
     5701 acattaggcg atgcgctggg ggccactgcg cagtcctgga acatgctgat ccgggagttc
     5761 gcggcggcgc ggcagcagtt ggagacggtg cggcgcgcca atcagacggg cgacattctg
     5821 acgcgttttg aaggggcggt attcagacga atggttgacc ggctgccctt cggcgtgctg
     5881 tgtgtgaact ccgaccagac ggttacgtat gccaacggct cggcgctggc cctgctggga
     5941 gcgcggagcg aggcggtgct gggccagccg ttaggcgaag cggtcggggc gccggccgtg
     6001 gccgcggcgg tcagcggagc gcaggcgcgt tccagcgccg gactggcgat cgatcacacg
     6061 ctgacgaccg gagagagcga gaccacgctg cgattccggg tgctgccgtt gtcggacgag
     6121 gcggcgggcg gcgagacgct ggtcagcatt gaagacattg ggcagctcaa ggaaagcgag
     6181 cgggcgcgcg acaactttct ctaccatgtt acgcatgagc tgcgcacgcc gctgaccagc
     6241 attcacgcct atgccgaaac cctgacgcga ccggggttcg acgacgagca gacgcgcaag
     6301 gaatgctaca acgtcatcat gagcgagacg cgccggctgt cgtcgctggt ggaagacatt
     6361 ctcagtttgt cgcaactgga ggtgggcacg gcgcgcctcg acgtgggcga cgtggacctg
     6421 gtacggctga tacggcagat ggtgcaggac aacctgggag cggcggacga gaagcgcatt
     6481 gacttgacgc tgacgctgcc gcccaaaacc cccaaagtcc gcggcgacaa gcagcgccta
     6541 tcggtgctgc tgatgaactt gatcggcaac gcgatcaagt acacgccgga gaacgggacc
     6601 gtacaggtga cgcttacggc cagcgagcag cgcgtcgcgg tcagcgtgcg ggacacgggc
     6661 atcggcatcg cgcccgagga ccaggcgcac gtcttcgaga aattctaccg ggcctcgaac
     6721 gacaaggtgc aggccgtcgc gggcaccggg ctgggtctgg cgatcgcgcg cgaggtggcc
     6781 cggctgcacg gcggggacat ccatttacag agcgagcccg gccagggttc gacgttcacc
     6841 gttgaactgc cggtctcgaa caccgagtcg gcggaggcgt ttgcgcgatg agccggattc
     6901 tggtagccga ggacgatccg cacattctgc gtctgatcgc gatgtggctg cgccgccagg
     6961 ggcacgaggt gctggaagcg cggcacgggg cggcggcgct ggcgttgatc gaagcgcagg
     7021 cggtggacgt gctggtgtcg gatgtgaaca tgcccggcgt ggacggtctg gagctgatcg
     7081 aagccatatt gccgcagagc cgcgtacggc gcggggtgat cgtactgacc aatcggtggg
     7141 atcacggcga aattcgcgag cgcttggcgc gttggggcgt gcaggtgatg cccaagccgt
     7201 tcagcccgac gcggctgtcg gaactggtcg aagcgctggt gcgggccgac ggtgggccgg
     7261 cgaaatccga agagaacctg gacctggcgg ccggcgcgac agagggtcag ttcgcgccac
     7321 cggcgagttt gccgccggcg tgatgaggag aggcgcagat gttcggctca gatgcgaaca
     7381 cgttgtggtt tgcggcgggg gcggccttgg ccgcggtggc cgtgctggcg ggtttcgcct
     7441 ttacgcggcg gcgcgacagt ctggcggagc cggcgccccc ggcggcgcgc gggttttcgg
     7501 cggcggcctt ggatttgggg cgccgcccgg ccggggtggc agacgccatg tgccgcagtt
     7561 ttcttgaatg gctagcggag cacgaggatc aggcagagct gtgggcgtca tttgaccaat
     7621 tggtgcgcga gctgttgacc gagcacctgg gagctaaccg ggtgcgctgt tatcacgtgc
     7681 gggccggggc cgaggcgttg caaccccttt ccggcggcca tggcgagatg gtgggggcca
     7741 gcagccggca tggggtgctg gggcacgtgg cgaccagcgg caaggagttt gtggccggcg
     7801 acccgggttg cgggccgctg ctggatgatc tggcggctcg tacggaggag ccctgggttt
     7861 ggatctggcc ggtgcgcgaa gcgggtggga cgctcggcgt ggttgcggtg ggcaacctgc
     7921 gtcaggttgg cgcgccgggc tttggcttgc ggcggacggt ggggcagctt ctgacgatat
     7981 tttggcggca cacggcgtgt gtggagcgct ggcgcgtggt gcagcgcacg gatcgcgggt
     8041 cgggcgtgtt gacgcgcaat gatttcttca cgttggcggc gcacgcgttg aatgattcgt
     8101 atcagacgaa cgaaccggtg gtagcggtcg tgctggcgct ggagggcatg cggcgtttgg
     8161 acgacagcgg acgctgggag gagcgcgacc gcctgattga gcggctgggc tgcgcgttgg
     8221 cgcggcgcgt gcgcagcgat gatctggtgg ggcgtttcgc cgacgatcgc tttgtggttc
     8281 tgctgcgccg cctggacagc ggactggggc gcctgattgc ggaaaagatg ctcgcagcgg
     8341 gggaggagtg tttggagcaa atggcgggcg ggcgcgaaaa cttacggctg cggctggggc
     8401 tggccggcag cggacacggg cagccgccgc tggagaagct attggtgacg gcttttgcgg
     8461 cggcggagcg ggcccgtcgc gccaacgtgc cgatcgcgac ggatttattg gaggccggcg
     8521 ggcgcggcgg cgctgcggca ggcgaaagcg cactcgagcc gcaagcaaag acagaggtga
     8581 gctcgacgtg aaagtgcaga cgcagcagca aggcaacgtc acggtggtgg gcctgcacgg
     8641 accgttgatc acggacgagt tggaggacgt gcgccgcgcg ttgcagaccg gcaatggcga
     8701 cttgcgccgc accatactcg acatgagcga cgtgccgtat ctggacagta acgggatcga
     8761 gctgttgctc gaattgtgcg gggcgcatct ttcgccgcac caacgcccga agatggcggc
     8821 cttgagcgaa acctgtctgg aagcgctcga attgacggac gtgttgcccc ggctggacgt
     8881 ttttgacacg gtcgaaaacg cgatccggag ctgccagcgg tgaggacgga cgagggtcaa
     8941 gatcacggcc ggcggcagga cgcggccgcc acaagccgag tgtgagcaaa aatgggccta
     9001 atgcgcacga acaaacttgg tcagctcctg gtggagcagg gtctggtcag cgaggctgat
     9061 ctactgcgcg ccctggaggc ccagaacaag gacaataagc gcctgggcga agtcttgatc
     9121 gacatgggca ttctgtcgcc caaggccatg ctcagcgcgc tggccaaaca actgaaggtc
     9181 aagtgctgcc atttgcggca cgggctgatc gatccggcgg tagcgcggct ggtggaccgc
     9241 gagcaggcgg cgcggctgcg ggtgctgccg ctcttcaagg tgggcaacac gctcaccgtg
     9301 gcgatggctg agccgcaatc actgccctcg gtggacttgc tgagtgcagt gactgggtgc
     9361 cagatcaacc cggtgttggc tctggagagc aacattgcgg aatttcagga gaagtacctg
     9421 ggggaggagg tggatattga ttctttcctc gtcaacctga ccgaatccga ggtcaaggtg
     9481 gtcgagcgcg aaacggtcga cgaggatacg ccgcaagaaa tcggcaagat ggtcgcgggc
     9541 agcccgatca tcaatctggt gaacctggcg attatgaccg ccatccgcga cggcgccagc
     9601 gacattcacg tggagccgac caagcgcaac acctgtattc gttatcgcat tgacggcaat
     9661 ctgcgcgagc tgatgacggc ccccaacacc atgcacgcgg cgatcgtgtc gcgcatcaag
     9721 gtcatcggca agatggatat tgccgagcgc cgcctgccgc aggaagg
//
LOCUS       DAOSYS010000136         9767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5931_length_9767_cov_5.056940, whole genome shotgun sequence.
ACCESSION   DAOSYS010000136 DAOSYS010000000
VERSION     DAOSYS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5931_length_9767_cov_5.056940"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PLU99_09530"
     CDS             <1..860
                     /locus_tag="PLU99_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17338.1"
                     /translation="GLALLAAWGLARLFERLRHSNKPARLRAALLAAILAVCAAEALT
                     TRTYLGYWRDSESLHRRAMTISPRAVQPHWHLAYLLRREGRLDEALEEYRAAVELAPS
                     VARLRHDFGSALAARGDTAGAVAQYRKALELDDTLVLTHNALALALTDQGQFTEAFAH
                     FEAALRVQPGLALTLNNYGVALEKAGRLGEAMAQYRAAVQAQPDFALAHSNLGEALSK
                     TGQPDEALRELRAAVQCAPGDARLRLRLAMQLERQNLFEQAAAEYRTILQLEPGHAQA
                     RARLAALGN"
     gene            904..2439
                     /locus_tag="PLU99_09535"
     CDS             904..2439
                     /locus_tag="PLU99_09535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOO17339.1"
                     /translation="MWPLLSRGDEAVRVRYWVALSLAGLALRAALIPVSPAWGYHRDH
                     DLFVRWAILATDYGLLTVYDGPPPKHDIQVWDAQTRSFVRTEEQQRDWCNYPPLSVYL
                     LYVSGLVFKVLSPERLINTWLSHAIFSSWTILADFLLAWGCAATVAHFRPGRAAFWTY
                     AAVLLAPPLWYDSLVWGQVDSWVLAPAVWMLWAILRQRWILAGVLYGIAAGLKPQAVL
                     FLPLWGLVLLTSRQPIKVLLSGLIAGAVLGLCALPFMLHSGWAWLKLSYIGNFFHAAP
                     YTTLSAFNIWYVDALLGTTKSATLTWLGLTKDVWGRTFLLIALAVGFVGVLIRWRREP
                     RGLLIWTVLSLLAFMMLPTRVHERYLVLVLPFLIMLAVLYARFVPTVVILALVMMAQI
                     TWPHWIKLRPGAWVDFFEPAARIDYEAYLTELPPQERARAQTYDEFTRDARQEFAATR
                     ARSAPFEWMGTILALLASAGVATTAATLKPPPAALQQTTRAERKTTGTEKQKGRIRGA
                     RSR"
     gene            2623..>2813
                     /locus_tag="PLU99_09540"
     CDS             2623..>2813
                     /locus_tag="PLU99_09540"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO17340.1"
                     /translation="MTNTEVARVFEDVADLLEIQGADVFRVNAYRRVARTINDLAVDI
                     NEIAQRGELAKLPGVGKSSA"
     assembly_gap    2814..2913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2914..4230
                     /gene="polX"
                     /locus_tag="PLU99_09545"
     CDS             <2914..4230
                     /gene="polX"
                     /locus_tag="PLU99_09545"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOO17341.1"
                     /translation="EGIEFIERSAGRVRLGIAHAIANSVCETVLALPGVRRVERAGSL
                     RRGCETVGDLDLLCVADDPEAAIRGFTQMPGVTHVSAAGRTKGSVLLEYQPRRTIQID
                     LRVVPEESFGAAWQYFTGSKAHNVRLRELAVKRGWSLNEYGLTEGPRVIAAATEEEIY
                     AALGVPCFPPELREDRGEFELRETPPDLLQLADIRGDLHVHTTASDGVNTAEEMIAAA
                     RARGYAYVCIADHSPSSTIANGLSPERLLQQIEMIRGLARRARGIKVWVGSEVDIRPD
                     GSLDYADELLAKLDFVIASIHFGMGHDREANTRRTLAVIENPYVNVIGHPTGRLIGKR
                     DAMPLDIEAVCRAAARSGTALEINASHMRLDLKDQHARLAREMGVTICINTDAHSIDE
                     YEEMQFGVMTARRAGLRRADVLNTWPADAIAKFVQAKRNYKPAHTR"
     gene            complement(4254..5054)
                     /locus_tag="PLU99_09550"
     CDS             complement(4254..5054)
                     /locus_tag="PLU99_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO17342.1"
                     /translation="MARKTGFTLVEVLVVIAVIATLAGLLLPVLSSARAAGRLTVCAS
                     NLRQIGLALHAYAHTNRGFLPRGPEPAHPFDFAASATATNQLWIGAGSDSGYPSPHPL
                     QYTGLGGLIAETLERRIYFCPTDSNYNLDQELPKIGSAADAYGSYLYRQLDQLPPEAA
                     GQLDQLGSNEIDGQVVKVEALALDMNSLGPPPFHQTNHEGQVVNLLFRDTSVRKFQNE
                     HDIFAIPASAFADFTRIPTAIDQLLISADYAYVGRPADAPRLTQDAEP"
     gene            complement(5151..5954)
                     /locus_tag="PLU99_09555"
     CDS             complement(5151..5954)
                     /locus_tag="PLU99_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOO17343.1"
                     /translation="MHIRGLLLDVEGVLVADKRYTAVPTATEFIARARAARVPLRLIT
                     NNTTDDKPAIIEKLRRAGFDFSLPELHTCTSAAINHLRTLSARRCFVMGNAALQEIFR
                     AAGFEVVNEAAVDAVVVGLDLDLSFERLTTACQAIVGHRAAFIALHRNRLYPDALGRV
                     APSVGAIVAAIEYATQVEPVLIGKPSPAYFKQALNELGIPPADVLVVSDDPLSDLAGA
                     KRMGMRAALVLSGKYPTAGVLEKLPPGERPDLTAPRIGDLLTSSLISLG"
     gene            complement(6010..6942)
                     /locus_tag="PLU99_09560"
     CDS             complement(6010..6942)
                     /locus_tag="PLU99_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17344.1"
                     /translation="MRPYTLLTLAVLVLVTPAGCPRGGFAENQNVSGGDENSTPSQDA
                     APDENGEAAAKRPNFDGAWVLMSGPETVACIEIDADVIVAWDDACVIAALDQALASTI
                     ATLTQDAESELGDLAGTRVYTGTNGELTGGYPWPEVELYDSDAPFGSLNVFSVTITAS
                     TPREGAPGVVDEWVGTYDAVKVNDSRLRLEIVLDNADIPVHALCHYTVLGKPRIRTVD
                     ECVAAGVAADVCANRFQQLADDIAVAQSDHDAGVLAAQVTVMSEPFVFADGTATWNIH
                     LTTASGGRTHSLSIDAGTDPPAGHFDGLPAMLVR"
     gene            7550..7855
                     /locus_tag="PLU99_09565"
     CDS             7550..7855
                     /locus_tag="PLU99_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17345.1"
                     /translation="MFLLPALFTLPLLAAFYLRLLPAEVVQMANQWQLPATVVIVAYY
                     FLVGLAVLLWLGWHAGKRDSDAPRCPQCGYSLKGSPGPRCPECGSACEAIPPKVEQP"
     gene            7842..8078
                     /locus_tag="PLU99_09570"
     CDS             7842..8078
                     /locus_tag="PLU99_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17346.1"
                     /translation="MNSHEVTNDTNVASLAQKREHSPSTGGSKLTTLSCARFFDDTGK
                     PVQIGIYSGAWVWIYNGKRVSSVQAVIRPKEAID"
     gene            8185..8258
                     /locus_tag="PLU99_09575"
     tRNA            8185..8258
                     /locus_tag="PLU99_09575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8219..8221,aa:Arg,seq:cct)
     gene            8465..8908
                     /locus_tag="PLU99_09580"
     CDS             8465..8908
                     /locus_tag="PLU99_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOO17347.1"
                     /translation="MLRISQRCEYAIRAMLELALRRDRRPVSIAEIAQAQRLPAKFLA
                     AILAQLKQAGLLESLRGTQGGYRLARQAHAISVGEVVRLMDGPMECTGATAAGAEATA
                     AGEVLKEIRAAIKGAVEQVLDNMTFADLVEREMRRREILAATYCI"
     gene            8920..>9767
                     /gene="cysK"
                     /locus_tag="PLU99_09585"
     CDS             8920..>9767
                     /gene="cysK"
                     /locus_tag="PLU99_09585"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOO17348.1"
                     /translation="MTHILDNVTLGIGNTPLVRLRKLIRSNASVLAKLEARNPMCSVK
                     DRIGLAMIEAAEREGRLRPDTVIIEPTSGNTGIALAFICAARGYRCLLTMPESMSLER
                     RKVLRALGAELVLTPAAGGMRVAVERAAELARETPNSFMPQQFENPANPEIHRRTTAE
                     EIWEATGGEVDIFISGVGTGGTLTGVAEVLKARRPGFRAIAVEPAQSPVLSQTLAGAA
                     PTPGPHKIQGIGAGFVPKVLNLKIVDEVVQIDQEEAIDWARRAAREEGLFVGISSGAA
                     LAAANQV"
BASE COUNT         1737 a         3046 c         3147 g         1737 t
ORIGIN      
        1 tgggattggc gctgttggcc gcctggggcc tggcacggct gtttgagcgg ctgcgtcaca
       61 gcaacaagcc ggcccggttg cgcgcggcgc tgctcgcggc aatcctggcg gtgtgtgcgg
      121 ccgaagcgct gacgacgaga acttatctcg gctactggcg agatagtgag tcgctgcacc
      181 ggcgtgcgat gacaatctcg ccgcgcgccg tccagccgca ctggcacttg gcgtaccttc
      241 tgcgacgtga gggccgcttg gacgaagcgc tggaggaata ccgggcggcc gtcgaacttg
      301 cgccaagcgt agcccggctg cgccacgact ttggctccgc cctagcggcg cgcggcgaca
      361 cagccggcgc cgttgcccag tatcgaaagg cgttggagct ggatgacact ctggtgctga
      421 cgcacaacgc cctcgccctg gctttgacgg atcaaggaca gttcaccgaa gcattcgcac
      481 atttcgaggc cgcgctacgc gttcaacccg gcctggcgct cacgctaaac aactatggcg
      541 tggcgttgga aaaagcgggg cgcttgggcg aggccatggc gcagtaccgg gccgccgttc
      601 aggcgcagcc cgatttcgcc ctggcacata gcaatctggg ggaggccctg agcaagacgg
      661 ggcagccgga cgaggcgctg cgcgagctgc gcgccgcggt gcaatgcgcc ccgggcgatg
      721 cgcgactgcg tttgcgtctg gccatgcagc ttgagcggca aaacctgttt gagcaggcgg
      781 cagcggaata tcgcacaatt ctccaactcg aacccggcca tgcacaggct cgcgcacggc
      841 tggccgcgtt gggcaattga gggacgccgc gggcgatggc caatcttcgg ggttcccgat
      901 agcatgtggc ctttgctgag ccgtggggac gaagcggtgc gtgtgcgtta ctgggtcgcc
      961 ttgtcgctgg cggggctggc gctgcgggct gcactgatac ctgtcagtcc ggcttggggc
     1021 tatcaccgcg atcacgatct gttcgtccgc tgggcaatcc tcgccaccga ctacggccta
     1081 cttaccgtct atgacgggcc gccgcccaag cacgacatac aggtttggga cgcccaaacg
     1141 cggagctttg tccgcactga ggagcagcag cgggactggt gcaactatcc gcccctatcc
     1201 gtgtacctgc tctatgtctc ggggctggtg ttcaaagtgc tcagcccgga gcgcctgatt
     1261 aacacttggc tctcgcacgc gatcttctca agctggacaa tcctcgccga ctttctgttg
     1321 gcctggggct gcgctgcgac ggtcgcccac ttccggccgg ggcgggccgc gttttggacg
     1381 tacgcggcgg tcctcttggc gccgcctttg tggtatgact cgctcgtttg ggggcaggtt
     1441 gacagttggg tgctggcgcc ggccgtatgg atgctgtggg ccattctgcg gcagcgctgg
     1501 attctcgccg gcgtgctcta cggcatagcg gccgggctca agccgcaagc cgtgttattc
     1561 ttgccgctgt ggggtttggt gctgctgacc agccggcagc ccatcaaagt gctgctctcg
     1621 gggctgatcg cgggcgcggt gctgggactc tgcgcgctgc ctttcatgct tcacagcggg
     1681 tgggcctggc tgaaactcag ctatatcggg aatttcttcc acgccgcccc gtataccacg
     1741 cttagcgcgt tcaacatctg gtatgtggac gcattgctgg gcaccacgaa gtcagcgacg
     1801 ctgacctggc ttgggctcac gaaggacgtc tggggccgaa ccttccttct aattgccttg
     1861 gcggtggggt tcgtaggggt gctgatccgc tggaggcgcg agccgcgcgg actgctgata
     1921 tggaccgtgc tgtcgctgct ggccttcatg atgttgccga cgcgcgtaca cgagcgctat
     1981 ttggtgttgg tgctaccgtt cctgataatg ctggcggtgc tgtatgcgcg ctttgtgccg
     2041 actgtcgtga ttctggcgct ggtcatgatg gcgcaaatca cgtggccgca ttggattaaa
     2101 ctgcggcccg gcgcttgggt tgactttttc gagcccgccg cacggattga ctacgaagcg
     2161 tatttgaccg aattgcctcc gcaagagcgc gccagagcgc aaacatacga tgaattcacg
     2221 cgcgacgccc ggcaggaatt cgccgcaacg cgcgcccgct cggcgccgtt cgagtggatg
     2281 ggaacaatcc tggcgctgct cgcaagcgcc ggggtggcta ccacggcggc taccctgaag
     2341 cccccgccgg ccgcgctgca gcaaaccacg cgcgccgagc ggaaaacgac aggaacagaa
     2401 aagcagaagg gcaggatccg cggcgcgcgt tcgcggtaag ccgccgctag acctctagaa
     2461 ttaatcccgg cgtttagcgc gccacgccgg gggcgccgct gcgggccttg ccagtgctta
     2521 atacggatgc ttagtatcag atccgtaggc gagccggcga gtcgctggtt cgcaggcggg
     2581 ccggcaagta tcagggggcg ttagccggcc ggcggagcca gtatgaccaa taccgaggta
     2641 gcccgggttt tcgaagacgt cgccgaccta ctggaaatcc agggcgcgga cgtgtttcgg
     2701 gtcaacgctt accgtcgcgt ggcgcgtacg atcaacgacc tcgcggttga catcaacgaa
     2761 atcgcgcagc gcggcgagtt ggccaagctg cccggcgtcg gcaagtcctc ggcnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngagggca tcgagttcat cgagcgcagc
     2941 gccgggcgcg tccgcctggg cattgcccac gccatcgcaa actcggtctg cgaaaccgtt
     3001 ctggcactac ccggcgtgcg gcgcgtagag cgggccggct cgctacggcg cggctgcgag
     3061 accgtgggcg atctcgatct gctgtgcgtc gccgatgacc cggaggccgc gattcgcggc
     3121 ttcacgcaga tgccgggcgt cacgcacgtg tcggccgccg gacggaccaa gggctcggtg
     3181 ctgctggaat atcagccgcg gcgcacgatt cagatcgatt tgcgcgtcgt gccggaagag
     3241 tctttcgggg cggcttggca atacttcacc ggcagcaagg cgcataacgt gcggctgcgt
     3301 gagctggcgg tgaagcgcgg ctggagtttg aacgaatacg gcttgaccga ggggccgcgc
     3361 gtgatcgccg ccgctaccga ggaggaaatt tacgccgctt tgggcgtgcc gtgttttccg
     3421 cccgagctgc gcgaggatcg cggcgagttc gagctgcgcg agacgccgcc ggacctgctg
     3481 caactggcgg acatacgcgg cgatctgcac gtacacacca cggccagcga cggagtcaac
     3541 acggccgagg aaatgattgc ggccgcccga gcgcgcggct acgcgtacgt gtgcatcgcc
     3601 gaccactcgc ccagcagcac gatcgccaac ggcctgtcgc cggagcggct gctgcaacaa
     3661 atcgagatga ttcgcggttt ggcgcggcgg gcgcgcggca tcaaggtgtg ggtcgggtcc
     3721 gaggtcgata ttcgccctga cggctcgctg gattacgccg atgagttgct ggcgaagctc
     3781 gatttcgtga tcgcctcgat acacttcgga atgggccacg accgcgaggc gaatacccgc
     3841 cgcacgttgg ccgtaattga gaacccgtac gtgaacgtca tcggccaccc gacggggcgc
     3901 ctgatcggca agcgcgacgc catgccgctg gatatagagg ccgtctgccg cgccgccgcc
     3961 cgcagcggca ctgcgctcga gattaacgct tcgcacatgc ggctggattt gaaagatcag
     4021 cacgcccggc tggcgcgcga aatgggtgta acgatctgca tcaacaccga cgcccacagc
     4081 atcgacgaat atgaggaaat gcagttcggc gtcatgacgg cccgaagagc cggtctgcga
     4141 cgcgcagacg tgctgaatac ctggccggcg gacgcgattg cgaagttcgt gcaggcgaaa
     4201 cgcaattaca agcccgcaca cactaggtaa tcgacttggt gtcctggtgg ggactacggc
     4261 tctgcgtctt gggtgaggcg cggcgcgtct gccgggcgcc ccacgtacgc gtaatccgcg
     4321 ctaatcagga gctgatcgat cgcggtcgga atgcgcgtga agtcggcgaa tgcgctggcc
     4381 ggtatcgcga agatgtcgtg ctcgttttga aacttccgca ccgacgtatc tcggaagagc
     4441 agatttacga cctgtccctc gtggttcgtt tgatgaaacg gcggcgggcc gagactgttc
     4501 atgtccaaag ccagcgcctc gactttgacg acctgcccgt caatctcgtt gctgcctagc
     4561 tgatcgagct gaccagcggc ctccggcgga agctggtcca gttggcggta tagataggag
     4621 ccgtacgcgt cggccgcgga cccgattttc ggcagttcct gatccaagtt gtagttgctg
     4681 tcggttgggc agaaatagat gcgccgctcg agcgtctcgg caatcaagcc ccccaggccg
     4741 gtgtactgga gcgggtgcgg agagggatag cccgaatcgg agccggcgcc gatccaaagc
     4801 tgattggtgg ccgtggcgct ggccgcgaaa tcgaaagggt gagctggttc cggaccgcgg
     4861 ggcagaaatc cgcggttggt gtgggcgtac gcatgcagag ccagaccgat ctgccgcagg
     4921 ttgctggcgc agacagtcag ccgacccgcg gcgcgggcgc tgctcagcac tggcaacagc
     4981 aacccggcca gcgtcgcgat caccgcgatc acgaccagca cttcgaccag tgtaaaccct
     5041 gttttccgcg ccatgtgcga actcgaccaa tcggctcgaa accgattgaa acagtgcacg
     5101 ggatgcgcca ctacactaac gcggattctc gtgaaaagga acgcgcgcgg ctagcccaga
     5161 ctgatcaggc tcgacgtcag cagatcaccg atgcgcggcg cggtaaggtc gggccgctcg
     5221 ccgggcggga gcttttcgag cacgcccgcg gtcgggtatt tgccggagag caccagcgcc
     5281 gcgcgcatgc ccatgcgctt cgcgccggcc aggtcggaca gcgggtcgtc ggagaccacc
     5341 agcacgtcgg ccggcgggat gccgagctcg ttaagcgcct gcttgaaata ggccgggctg
     5401 ggcttgccga tcaggaccgg ctcaacctgc gtggcatact cgatagcggc gacgatcgcg
     5461 cccacgctcg gcgcgactcg cccgagggca tctgggtaca gccggttgcg gtgcagcgcg
     5521 atgaacgccg ccctgtgacc gacgatcgcc tggcacgccg tagtcagacg ctcgaaactc
     5581 aaatccagat cgagccccac aaccacggca tccacggccg cttcgttgac gacttcaaag
     5641 ccggcggccc gaaaaatctc ctgcagtgcg gcattgccca ttacaaaaca gcggcgggcg
     5701 gacagcgtgc gcaggtggtt tatcgccgcc gacgtgcacg tgtgcagctc cggcagagaa
     5761 aagtcgaagc cggcgcggcg gagtttctcg ataatggccg gtttgtcgtc ggtggtgttg
     5821 ttagtgatca ggcgcagcgg cacgcgcgcc gcccgggccc gcgcgatgaa ctcagttgcg
     5881 gtggggacgg ctgtatagcg cttgtcggcg acgagcacgc cttcgacgtc cagcaataga
     5941 cctctgatgt gcatctttag ctccgcatgt gcccataagc ccatggaact gtcgcgatct
     6001 cgggcgtgct cagcgaacga gcatggccgg aagcccgtcg aagtgaccgg ccggcggatc
     6061 tgtcccggcg tcgattgaaa gcgaatgcgt gcggccgccg gatgcagtag tcaaatgtat
     6121 attccaggtt gccgtgccgt cagcgaacac gaacggttca gacatgacgg tcacttgcgc
     6181 cgccagcacg ccggcgtcgt ggtctgattg cgcgactgcg atgtcgtcgg ctagctgctg
     6241 aaagcggttc gcgcacacat cggctgccac cccggcggcc acacactcgt caacagttct
     6301 tatgcgcggc ttgccgagca cagtgtaatg acagagcgca tgtactggaa tgtcggcatt
     6361 atcgagcaca atctcaagtc gaaggcggct gtcattcact ttcacagcgt catacgtccc
     6421 gacccattcg tctacaaccc caggcgcgcc ctctcgcggc gtcgaagccg taatcgtgac
     6481 tgagaagacg tttaacgaac caaagggcgc gtcagagtcg tacagttcca cctctggcca
     6541 cgggtagcca cctgtcaatt ccccattggt tcccgtgtaa acccgcgtac ccgcaaggtc
     6601 gcccaattcg ctctcggcgt cctgcgtgag cgtggcgatt gtgcttgcca gggcctgatc
     6661 aagggcagcg atgacgcagg cgtcgtccca cgcgactatc acatctgcgt cgatttcgat
     6721 gcacgccacc gtctcaggtc ctgacatcag aacccaggcg ccgtcgaaat tcggccgctt
     6781 ggcggctgcc tccccgttct cgtcgggcgc ggcgtcctgg ctcggggtgc tgttctcgtc
     6841 gccgccgctg acgttttggt tctcggcaaa gcccccacgc gggcaacccg caggcgtcac
     6901 gagtaccagc accgcaagcg tcaagagagt gtacggcctc atcatcattc atctcagtct
     6961 tacttgtccg ggcgtgccca acctgtgact gcgagccaca atcaagcttg ggcagcatcg
     7021 ccctgaaccg tagcacaacc cgccgggcga ggtcaacccg ttattgtcac gatcagtgag
     7081 gcaaatgccg atgttgctgg gtaaccagac gcaaccttgc gctcgtggcc ctcgcaatcg
     7141 ccttcaagct cacgtacgat gggtcccgcg acgcgcgccg cgcccaacgg cgacatactc
     7201 atgggagcaa cgcgtgtcgg gctgcggcga aggctagcac gcctgccccc gatgcgcagc
     7261 gctgcccgca atgcaatcat attgcatggc caatatagcg agttggcatg ttgagactgt
     7321 ggctcaggat cggaccagcg gctttcatag ccggcttcgc actcgcgctc tttgagcagc
     7381 tatgctggcg atggtccgct tctgccggca ttcatcgcct cggcgcctgc gcgcctgggg
     7441 gcttgtctgc gcggccgcac caaccatccc actatggctg gcgcgtcggc gcggcgcaca
     7501 gcctagggcc ttcagtcccc aactgacacg ttgcgcgcgt ggttcgacca tgttcctcct
     7561 gcctgcgctc ttcacccttc cgctactagc ggctttctat ctgcgtttgc tgcctgcgga
     7621 ggtcgtgcag atggcaaacc agtggcaact accagccacc gtcgttatcg tcgcgtacta
     7681 ctttctcgtg ggcctggcgg tcctcttgtg gctcggctgg cacgcaggca aacgtgactc
     7741 cgatgcgccg cgctgcccgc agtgcggcta cagcctgaag gggtcgccag gaccgcgctg
     7801 cccggagtgc ggctcggcgt gcgaggcgat acccccgaaa gttgaacagc catgaagtca
     7861 caaacgacac gaacgttgcg agtctcgcgc aaaagcgcga gcactcaccc tcgacagggg
     7921 gtagcaagct caccaccctg tcgtgcgcga ggttctttga tgacaccggc aagccggttc
     7981 aaatcggcat ctacagcggg gcctgggttt ggatctacaa cgggaaacgc gtgtcgagcg
     8041 tccaggccgt catacgcccc aaagaggcga tcgactaacc agcaaggaaa attggggacg
     8101 aagagccagc cggccggcgc ttcctccccg acttgctccg gctgaggcgc agggccacgg
     8161 agctggtaca atgacggcag aacagcgccc gtagctcagc aggatagagc accggtttcc
     8221 taaaccgggg gtcgcaggtt cgaatcctgc cgggcgcgtt gttcccgcac gccctgtcat
     8281 ccaaccagtc gcgtccggta actcagtgtc taacgggact ttccacggaa ctttcggccg
     8341 caggttctgc cctgaggcga cgccacacct ctcgatccct catatccctc aatccctcaa
     8401 aaaaattgac ttgactatca ataagcggat actctactat atcgatagat gaagtgaagt
     8461 aaccatgctc cgtatctctc aacgttgcga atacgccatt cgagccatgc tcgaactcgc
     8521 gctccgccgc gatcgccggc ccgtctcgat cgctgagatc gcccaagcgc agcgtctgcc
     8581 ggccaagttt ctcgccgcca tcctggcgca gttgaaacag gccggcctac tcgaatcgct
     8641 acgcggcacg cagggcggtt atcgcctggc gcggcaagcg cacgcgatct ccgtgggcga
     8701 ggtggtgcgg ctgatggacg gccccatgga gtgcaccggc gcgaccgccg cgggcgctga
     8761 ggccaccgcg gccggcgagg ttctgaagga aatccgcgcg gcgataaagg gggccgttga
     8821 gcaggtgctg gacaacatga ccttcgccga cctggtcgaa cgtgaaatgc ggcgccggga
     8881 aattttggcg gccacctact gcatctagga ggaacggaaa tgacgcatat tctggacaac
     8941 gtcacacttg gcatcggcaa cacgcccctg gtccgcctgc gcaagctgat ccgctcaaac
     9001 gccagcgtgc tggccaaact cgaagcccgc aacccgatgt gctcagtcaa ggaccgcatc
     9061 gggctggcga tgatcgaggc ggccgaacgc gaggggcgct tgcggccgga cacggtcatc
     9121 atcgaaccca ccagcggcaa cacgggcatc gcgctggcgt tcatctgcgc cgcgcgcggc
     9181 taccgctgcc tgctcaccat gccggaatcg atgagccttg agcggcgcaa ggtgctgcga
     9241 gccctcggcg cggagctggt actgacgccg gcggccggcg ggatgcgcgt ggccgtggag
     9301 cgagcggccg aactcgcccg cgagacgccc aacagcttca tgcctcaaca gtttgagaac
     9361 cccgcgaacc ccgaaatcca ccgccgcacc acggccgagg aaatctggga agccacgggc
     9421 ggtgaagtgg atattttcat cagcggcgtc gggactggtg gaactctcac cggcgtggcg
     9481 gaagtgctca aggcacgccg gccgggcttc cgcgcgatcg ccgtcgaacc ggcgcagagc
     9541 ccggttctgt cacagacgct ggcgggcgcg gcgcccacac ctggcccgca caagattcag
     9601 ggcatcggcg ccggctttgt gccgaaggtg ctgaacctga agatcgttga cgaagtggtg
     9661 cagatcgatc aggaggaagc gatcgactgg gcgcgccgcg cggcacgcga agagggcctc
     9721 tttgtcggaa tttcctcggg cgcggccctg gcggccgcga accaggt
//
LOCUS       DAOSYS010000137         9763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_5936_length_9763_cov_5.050268, whole genome shotgun sequence.
ACCESSION   DAOSYS010000137 DAOSYS010000000
VERSION     DAOSYS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5936_length_9763_cov_5.050268"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLU99_09590"
     CDS             complement(<1..288)
                     /locus_tag="PLU99_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17349.1"
                     /translation="MGKLGAASCAIALILLYTQRRDRRLVARAPLAGAALACAALVCY
                     AAWRYFLRPGPVGRADGHVVRFILGACSGFAVPLALAWGRALVLQDVPALAR"
     gene            complement(424..1521)
                     /gene="hppD"
                     /locus_tag="PLU99_09595"
     CDS             complement(424..1521)
                     /gene="hppD"
                     /locus_tag="PLU99_09595"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOO17350.1"
                     /translation="MTAADFLPISGWDHVEFYVGNARQAAHFYEKTFGFEPVAKVALE
                     TGSRDRASYVLKQGDIRFVFTSALVPDHEIARHCQLHGDGVKTIALAVRDCDAAMNAV
                     RLRQATILQPSQEAADEHGVIKTGVIKYAGDTVIKFVERGGYRGAFAPGYKPLRVAAP
                     TVPQVGLRAIDHVVTNVYLGEMNYWAAWFERVLGFKQLRHFTDKDISTEYSALMSKVM
                     EDGRQIKLPINEPAEGRRKSQIDEYLEWYRGPGVQHIALSTSDICHAVRMLRERNIDF
                     LRVPDTYYADLQARVGKIDESYNDLRELGILVDRDEDGYLLQIFSQPVQDRPTLFFEV
                     IERHGSRGFGRGNFKALFVALEREQERRGNL"
     gene            1654..3363
                     /locus_tag="PLU99_09600"
     CDS             1654..3363
                     /locus_tag="PLU99_09600"
                     /inference="COORDINATES: protein motif:HMM:NF017142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17351.1"
                     /translation="MRLAFPDVSDVNGGVKISYPLACVLLLTSLPAWSQNSEVVRYEL
                     APQVETGGVRVEMSWDTAGRTLSAVFIAPRWGTVNDVPGLIRNLQFGGGVTGVRKQET
                     VWLLQHGRDATITCTYEIDPGRRTLDWDAPHLPVTTRTFFHGVGNTFLIVPIAGAGMP
                     EKYNFALRWRLPPRWTAICSWGSGPAAGEELTPTDLRDSVYFAGRVDSRTVERDGRTI
                     TVALVDRFEFGVDEFSALAAKILEYECRFMGETEFPRLMVAAAPVGEAIGANDSRLVG
                     MGLYRSFDLLAAPASTLNDGFESLFAHELFHHWNGGVLKAGQPDKLVYWFIEGFTEYY
                     SLRILYESGHWDAKTYAKWINRHLRQYHNNPAANATNEQIAERYWRERDSVGEIPYQR
                     GVLLGLRWHRLARSQGTPDGLDRLFKALVALGRTGEFELSNAAIREVGVRVLGDWFGA
                     EFDRYVTAAETITVPRDALAPEFVADLEEVYEYQLGFDANKAAKDKRVRKLVRGSAAE
                     KAGVREGDQLVAAKVHADPDQPIELAVKRGEEVRQIRYYPRGRRTLALQFQVADSHTL
                     SAP"
     gene            3623..4855
                     /locus_tag="PLU99_09605"
     CDS             3623..4855
                     /locus_tag="PLU99_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOO17352.1"
                     /translation="MGRTPDGYDVRNLARPWVWTAAKHKSLTSWKCGRAAVWPALLVS
                     LCLMGCKADRRISMAQLAELEAQASMRPAVPVEPAKLELSELHGYRIQPRDVLVVRMI
                     GLSTADRYAETMIPVRVGEDGKVVLPVVGPIQAAGLELGEFEREVIAAHVPAVVRDLT
                     AYVEVAGPEATTVLVLGAALTPGLVRLPQNQRDILYALSSAGSFGPTSSLRVRLRPIR
                     PERPEVVYDFLDQNDVRRALQAPPLESGDTLVVEAEGNPAVYVAGLVNNPGAVLVPTR
                     STISLMGAIAASGGLVDFLEPDDATLWRTLDNGQRVQVKLDLAAIQSGCEPDPDLRPG
                     DVVFVPPSLKTRIRQWAMENLRFGPFGMTAVYDPIAIYEARLISNNNNNGARAAVLQG
                     ITSGAAEVIVPPVVAPAP"
     gene            4931..5632
                     /locus_tag="PLU99_09610"
     CDS             4931..5632
                     /locus_tag="PLU99_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO17353.1"
                     /translation="MRNILMAGTLGVFLVQVAGCPNMMTEGTSPGAKFTTSMGEFVVK
                     LELEKAPVSVGNFIQYAKAGHYDGTIFHRVVPGFMIQGGGYTPEMTLKEARANILNES
                     GNGLSNLRGTIAMARGEDPDSARAQFFINVADNKQLDSTLNTPGYTVFGQVMQGMDVV
                     DSIAAVPTRDEIGFSNLPVEPVVIQTVELIEVPDGTSELTPYGEQTVEQQRYQAMLFL
                     RETAVSLLQFALMPR"
     gene            6379..8703
                     /locus_tag="PLU99_09615"
     CDS             6379..8703
                     /locus_tag="PLU99_09615"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17354.1"
                     /translation="MDDRIVGWSEGCRRLYGYDFDEAEGRVCHALLRTRFPQPLSQIY
                     ETLREQGWWEGELTQRRSDDAVVVVRSKWTLRRTGGRKTVVVADTDITQQRRTAERLA
                     ASEELSRILMETMSQGVMQQDQDGRIAGVNPAAERILGRSVAELLGKTPADLEWHMLR
                     EDRTPLPEQEYPGLMALRTGQRVNDALVATWNPHARAYRWLSMDAVPLCRPGQSRPYQ
                     VYTIFADVTEGQRADAAAQHAAQRHETALDAAIMGTWDYHPENGSMFWDQRCRSIFGV
                     AFGDRVEYQTVIALVHEEDRARVEAEFNAALDSASSGAYESEFRVVWPDGSVHWVNLI
                     GQAYFKGLGGQRHAVRFIGTALDITTRKRLEEERDALLASERAARTEAERANRLKDEF
                     VATLSHELRNPLNIILGWVQILERSKRTPQEVSEGLQIIHGSGRVLADLISQLLDMSR
                     ITSGKLLLDMQTIDLGAVVAAALETAKPDIAAKDLRLETAFERAALRARGDPNRLKQV
                     VWNLLSNAVKFTPRGGLIRVETNRCGSRVEIRVSDTGQGIDPEFLPFLFERFRQEDAS
                     AARKHGGLGLGLSIARSLVEMHGGTISVESAGKGLGATFTISLPALADAEDGAQTQDR
                     SQAPGESTGAERAEAALDSFKILVVDDDRSSLEIVKRVLEERRATVFTAASARQGLEL
                     LQRFRPDVLISDIGMPEEDGYRFLRRVRALGADRGGSTPAMALTAFASPEDQHRAMIC
                     GYQAHLTKPIEAGRLIANVVRLLNQTRENGSAKS"
     gene            complement(8754..9212)
                     /locus_tag="PLU99_09620"
     CDS             complement(8754..9212)
                     /locus_tag="PLU99_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOO17355.1"
                     /translation="MSSVKASFIRSRRPHVPGLALLMGLILLIAPLAGCVERTIKIQS
                     EPPGALVTVNDEEVGATPVKFAFLWYGDYEIILRKPGYETLKTHCTIKPPWYQYPPID
                     FVAEALVPAMLQDNHVLPTFELEPAETPTVSEVVERATELRAETVCEQPE"
     gene            complement(9209..>9763)
                     /locus_tag="PLU99_09625"
     CDS             complement(9209..>9763)
                     /locus_tag="PLU99_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO17356.1"
                     /translation="RRSQAERIKSALHAFFGAAFSTSLPMRIGVLSDTHDRLPAIRAA
                     LERFSQLGLHTLIHPGDIVAPFAARVLATWPGELYVTFGNNDGERAGLQKVLPQIQDG
                     PLFISRNGKTILVQHFIDWCRAEDVRKADVIITGHTHEFGVHEKDGRLFLNPGECCGW
                     VTGHCTIAVLDLDKRQIEQIEITP"
BASE COUNT         1822 a         2774 c         3314 g         1853 t
ORIGIN      
        1 ccgcgccagc gcgggcacgt cctgcaacac cagcgcgcgg ccccacgcca gcgccagcgg
       61 caccgcaaaa ccgctacaag cccccaggat gaagcgcacc acgtggccgt cggctcggcc
      121 aaccggtccc ggccgcagga agtagcgcca ggctgcgtag cacacgaggg cggcgcaggc
      181 caaggcagcc cccgcgagcg gcgcccgagc gaccagccgc cggtcccgcc tttgcgtata
      241 aagcaggatc agtgctatag cgcagctcgc ggcacccaac ttgcccacgc gcgttcaagc
      301 tccgcaaaac cgcccgtgcc cggccgcgtt tgcgcgccgc gcggccacca tcgtagccgt
      361 ttgcggatca gataggaacc gctcagcggc ccgcgcgtcc gcaggcccgc cgggtgcaca
      421 gggttagagg tttccgcggc gctcctgttc gcgctccagc gcgacgaaca gggctttgaa
      481 attgccccgg ccgaagccgc gagagccgtg gcgctcgatc acctcgaaga acagtgtggg
      541 acggtcctgg accggctggc tgaagatctg gagcaggtaa ccgtcttcgt cgcggtccac
      601 gaggatgccc agttcgcgaa ggtcgttata gctttcatcg atctttccga cgcgcgcctg
      661 caggtcggca tagtaggtgt cgggtacacg cagaaaatcg atgttgcgct cgcgcagcat
      721 acggaccgcg tggcaaatgt cgctggtgct cagcgcgatg tgctgcacgc cggggccgcg
      781 ataccactcc aggtattcgt caatctgcga ttttctacgg ccttcggcgg gctcgttgat
      841 gggcagcttg atctggcggc cgtcctccat gaccttgctc atcagggcac tgtattcggt
      901 gctgatatcc ttgtcggtga agtgccggag ctgcttgaag cccaggacgc gttcgaacca
      961 ggccgcccag tagttcatct ccccgagata gacgttggtg acgacgtggt cgatcgcgcg
     1021 taagccaacc tgcggcacgg taggggctgc aacacgcaac ggcttgtatc ccggggcgaa
     1081 agcgccgcgg tagccgccgc gctcgacgaa cttgatgacg gtatcgcccg cgtatttgat
     1141 gacgccggtt ttgatgacgc cgtgttcatc cgcggcttct tgtgatggtt gcaggatggt
     1201 ggcttgtcgt agtcgaacgg cgttcatggc ggcgtcgcaa tcgcggaccg caagggcgat
     1261 ggtctttacg ccgtcaccgt gaagctggca gtgccgcgcg atttcatgat cgggcacaag
     1321 cgcgctggta aagacgaaac ggatgtcgcc ttgtttaagg acatagctgg cgcggtcgcg
     1381 tgaaccggtt tcgagggcga ctttggcgac cggctcgaag ccgaaggtct tctcgtagaa
     1441 gtgtgccgcc tggcgggcat tgccgacgta gaactcgacg tgatcccagc cgctgatcgg
     1501 gagaaagtcg gcggcggtca tgcttatggt ccgttacctt cctcgacgag ctgcgcggct
     1561 taccgacgca ggcgaccggg caggctgcgg cttggaggag gcaagccacg gcgtgagatt
     1621 atttgcgtta gcggggactt ttcagcatga gttttgcggc ttgcgttccc ggacgtgagc
     1681 gacgtaaatg gtggggtgaa gatttcgtat ccgttagcgt gcgtgcttct gctgactagc
     1741 ttgccggcat ggagccagaa ctcagaggtg gtccgctacg aactagcgcc gcaggtggaa
     1801 acagggggtg tgcgggtgga aatgagctgg gacacggccg ggcgcacgtt gtccgcggtg
     1861 ttcatcgccc cgcgctgggg cacggttaac gacgtgccgg ggctgattag gaatttacaa
     1921 ttcggtggcg gcgtgacggg ggtgcgcaag caggagacgg tgtggctgct gcagcacggg
     1981 cgtgacgcga ccattacctg cacctacgag atcgaccccg ggcggcggac gctggattgg
     2041 gacgcgccgc acctgccggt gactacgcgc actttttttc acggcgtggg caacacgttt
     2101 ctgatcgtgc cgatcgccgg agccggaatg cccgagaagt acaactttgc cctgcgctgg
     2161 cgtctgccgc cgcgttggac ggcgatctgc tcgtggggca gcgggccggc ggcaggggag
     2221 gagctaacgc caaccgacct cagggactcg gtgtactttg cgggccgggt cgactcgcgg
     2281 accgtggagc gcgacgggcg tacgatcacg gtggcgctgg tcgaccggtt cgaattcggg
     2341 gtggacgagt tcagtgcgct ggcggccaaa atcctcgaat acgaatgccg gttcatgggg
     2401 gagaccgaat ttccgcggct gatggtggcg gcggcgccgg tcggcgaggc gatcggggcc
     2461 aatgattcgc ggctggtggg gatggggctt tatcgcagct tcgatttgct ggcggcgccg
     2521 gccagcacct tgaacgacgg gttcgaaagc ttattcgcgc atgagttgtt ccaccactgg
     2581 aacggcgggg ttttgaaggc cggccagccc gacaagctcg tatactggtt catcgagggg
     2641 ttcacagaat actactcgct gcgaatcctg tacgaatccg gccactggga cgccaagaca
     2701 tatgccaagt ggatcaaccg gcacttgcgg caataccaca acaatcccgc tgcgaatgct
     2761 acgaatgagc aaatagcgga gcgttactgg cgcgagcgcg acagcgtggg cgagataccc
     2821 taccagcgcg gcgtgctgct gggtctacgt tggcaccggc tggcgcgctc ccaaggaacg
     2881 cctgacggcc tggaccggtt gttcaaggcg ctggtcgcgc tggggcgcac cggcgagttt
     2941 gagctttcaa acgcggccat tcgcgaggtc ggcgtgcgtg tgctgggcga ctggttcggg
     3001 gcggagtttg accgctacgt gacggcggcg gaaaccatca ccgtgccgcg ggacgcgctg
     3061 gcgcccgagt tcgtggctga cttggaagag gtttacgagt accagttggg cttcgacgct
     3121 aataaggcgg ccaaggacaa acgcgtgcgc aagctggtac gcggctcagc cgccgagaaa
     3181 gcgggcgtgc gcgaaggtga tcagcttgta gcggccaagg tgcacgcaga cccggatcag
     3241 ccgattgaac tggcggtgaa gcggggtgag gaggtgcggc agatccgcta ctacccgcgc
     3301 gggcggcgga cgctggcgct gcaattccag gtggcggact cgcatacact tagcgcacct
     3361 tgaacggcct gcctctggtg gcgccttaaa tgctggatat acctctgctc tgtagtcgcc
     3421 gccaaataac acaattgtca catcttacac gctaatcatc taataacgga cttggaaaac
     3481 ttaacgctgc aagccaataa ttcgagtgtt agggcgcgcg cctggcgcgg ctagggcgcg
     3541 gccggacagt ttattcaggc gaactatcga aaccgtcagc gccgcgccgc tcggctgcgc
     3601 gacggtctgt caggagagga ttgtgggacg cacccccgac ggctatgacg ttcgaaatct
     3661 cgcacgcccc tgggtttgga cagccgccaa acataaatcg ctaacaagtt ggaaatgcgg
     3721 ccgcgctgcg gtgtggccgg cgctgctggt cagcctgtgc ctgatgggct gcaaggctga
     3781 tcggcgcatc agcatggcgc agcttgcgga gctggaggcg caggccagca tgcgtccggc
     3841 cgttccggtc gaaccggcca agctggagtt gtcggaactg cacgggtacc ggatacagcc
     3901 gcgcgacgtg ctagtcgtgc gcatgatcgg tttgtcgacg gccgaccgct acgcggagac
     3961 gatgatcccg gtgcgcgtgg gcgaggatgg caaggttgtg ctgccggtgg tggggccgat
     4021 ccaggccgcg gggctggagc tgggcgagtt tgagcgtgag gttatcgcgg cccacgtgcc
     4081 ggcggtagtc agggatttga cggcttacgt ggaagtggcc gggccggaag cgaccacggt
     4141 actggtactg ggcgcggcgc tgacgccggg cctggtgcgg ctgccgcaga accagcgcga
     4201 cattctctac gccttgtcga gcgcgggcag ttttgggccg accagctcgt tgcgtgtgcg
     4261 gttgcgaccg atccgtccgg agcgcccgga agtggtgtac gatttcctcg accagaacga
     4321 tgtgcgtcgc gcgctgcaag caccgccgct cgagtcgggt gatacgctgg tagttgaagc
     4381 cgagggcaac ccggctgtgt atgtggccgg cctggtaaac aaccctggcg ccgtgctggt
     4441 accgacccgt tcgaccattt cgctgatggg ggcgatcgcg gcctcgggcg gtctagtgga
     4501 tttcctggaa ccggatgacg ccacgttgtg gcgaacgctg gataatggcc agcgggtgca
     4561 ggtgaagctt gacctggcgg ccattcagtc cggttgtgag ccggatccgg atttgcgacc
     4621 gggcgatgta gtgtttgtgc cgccgtcgct caagacgcgt atacggcagt gggcgatgga
     4681 gaacctgcgc tttgggccgt tcggtatgac tgctgtatat gatccaattg cgatctacga
     4741 agcccgtttg atttccaaca acaacaacaa cggagcccgg gcggcggtgc tccaagggat
     4801 tacgtcaggg gctgcggaag ttattgtgcc accggtagtc gcccccgcgc cgtagcatag
     4861 cattgaagtg gtgaacggga gtctggcggg ggcataggcg ctgcgaggcg gcgcgagtcg
     4921 agaggatcgt atgagaaaca tcctgatggc tggcacgttg ggcgtgttcc tggtccaagt
     4981 ggcaggatgc ccgaacatga tgacggaggg cacgagtccg ggcgcaaagt tcacgacatc
     5041 aatgggcgaa tttgtagtca agcttgagtt ggaaaaagca ccagtatccg tcggcaattt
     5101 catacagtat gccaaggccg gtcattacga cggcacgatt ttccaccgcg ttgttcctgg
     5161 gttcatgatt caaggcggag gttacactcc cgaaatgacg ctgaaggagg cgcgcgccaa
     5221 cattttgaac gagtcgggaa atgggctgtc caacctgcgc gggacgattg ccatggcgcg
     5281 cggcgaggac cccgattcgg ctcgcgccca gttcttcatc aatgtggccg ataacaagca
     5341 gcttgactcg acgttgaata caccgggtta caccgtgttt ggccaagtga tgcagggtat
     5401 ggatgtggtt gacagtatcg ccgccgtacc cacacgcgac gaaatcgggt ttagcaatct
     5461 gccggtggag ccggtggtta tccagactgt ggagctgatc gaggtcccgg atggcacgtc
     5521 cgagctgacc ccttacggcg aacagacggt cgagcagcag cggtaccagg caatgttgtt
     5581 tctgcgcgaa acggcggtgt cattgctgca attcgcgcta atgccgcgct gaacaaccga
     5641 tactcgcacc gagtctgtag tctttaatct atagactaca taacaacatg tgtagggctt
     5701 tagtctaact gtcatgggag gtgcggctgt tccctgattg tgaatatccg agaccgtgtg
     5761 tgtcttagga tatgacgaca ttataaacaa attgccagaa ggggggcttg ccaaatacgc
     5821 gttggcccaa tataactatc ggacatcgtt gggtgatgga ttcgcatccg tcggcggcag
     5881 gagcataccg ctttgtggcc accgaacagc tctcaaatcg tagaaaataa ctcgccaact
     5941 cttgtgtcga ggcgagtcgg tgcgtcctgg ggcgtgttcg gcctggtcgc ggccggtggc
     6001 ttgtggcgat atttgttcgc ggcggtcgtg gtcgcgctgg ttagtgtggc ccaaacctgg
     6061 ctgccaaaca caccggagac gcgcctactg tcgctggccg gtctttgtgg ggctgtagcg
     6121 ctggtggcaa gtattgcggg ggccgggcct ggccttttag caacgcttct agcagtcgtg
     6181 gcggtggagt gttggcttca agccgccttg ggtatcggcg gatgggacca tgtggtcggg
     6241 ctggccgctt tcattggcgg cagtctcttt gtatgcggcc ttgctcaagg ggtgcggcat
     6301 tttcgagata ggtcactcag tcgtttgcga ccgacgggac gtaaccagtt aggtttcggg
     6361 aacgtctcgg ttcgcgactt ggacgatcgc atcgtcggtt ggagcgaggg ctgccggcgg
     6421 ttgtacggat acgacttcga cgaagcggaa ggtcgtgttt gccacgcgct attgcgcacg
     6481 cgattcccgc agccactttc gcagatttac gaaacgctgc gggagcaagg ttggtgggag
     6541 ggggagttga cgcagcgtcg cagtgacgac gccgtggtag tcgtcagaag caagtggacg
     6601 ctgcgccgca ccggcgggcg caaaaccgtc gtagtcgcgg acaccgatat tacgcagcag
     6661 cgacgcacag cagaacgtct cgcagccagt gaggagctga gccggatact catggagacg
     6721 atgtcgcagg gggtaatgca acaggaccag gacggccgaa tcgcgggagt aaaccccgcc
     6781 gcggaacgga tcctgggacg cagcgtcgcg gaactgctgg gaaagacgcc ggcggacctg
     6841 gaatggcaca tgctgcgcga ggaccgtacg ccgctgcccg agcaggaata tcccgggtta
     6901 atggcgctga gaacggggca gagagttaac gacgccctgg tcgcgacctg gaatccccat
     6961 gcgcgggctt accgctggct gagcatggac gctgtcccgc tctgccggcc cgggcagagc
     7021 cggccatacc aggtctacac gatcttcgcc gacgtgactg agggccagcg ggcggacgcg
     7081 gccgcgcagc acgccgcgca gcggcatgag acggcgctgg acgccgccat catgggtacg
     7141 tgggattacc atccggaaaa cggcagcatg ttctgggacc agcgctgccg ctccattttc
     7201 ggcgtggcgt tcggcgatcg ggtggagtac cagacggtca ttgcgctggt gcatgaggag
     7261 gaccgggcgc gcgtcgaggc cgagttcaat gccgcgctcg attcggcctc atccggagcc
     7321 tacgaatccg aattccgtgt ggtttggccg gacggatccg ttcactgggt caatcttatc
     7381 ggtcaggcct acttcaaagg attgggcggg cagcggcatg cggtgcgctt catcggcacg
     7441 gcgctggaca tcacgacccg caagcggctt gaggaggaac gcgacgcgct gctggcgagc
     7501 gaacgggccg cccgtaccga ggcggagcgc gccaatcgcc tgaaagacga attcgtggcg
     7561 actctttcgc acgagctgcg aaatccgctg aacatcatcc tgggctgggt gcagatcctg
     7621 gagcgctcaa aacgcacgcc gcaggaggtg agcgaggggt tgcaaatcat ccacggcagc
     7681 ggccgcgttt tggcggacct gatcagtcag ttgctcgaca tgagccgcat cacttcgggc
     7741 aagttgctgc tcgacatgca gactattgat ttgggggcgg tggttgcggc ggccctggaa
     7801 acggccaagc cggacattgc ggccaaagac cttcgtctgg aaacggcgtt cgagcgggcg
     7861 gccctgcggg cgcgtggcga tccgaaccgc ctgaagcagg tggtttggaa cctgttgtcg
     7921 aacgccgtga aatttacgcc gcgcggaggg ctgatccggg tcgaaacgaa ccgctgcggt
     7981 tcgcgcgtgg agattcgcgt ttcagatacg gggcagggca tcgatcccga gttcttgccg
     8041 tttctgttcg agcgctttcg acaggaagac gcctccgcgg cccgcaagca tggcgggctg
     8101 gggctgggcc tgtcgatcgc acgctcgctg gtggagatgc acggcggcac aatcagcgtg
     8161 gaaagcgcgg gcaaaggact gggcgcgacg ttcaccatta gcctgccggc actggcggac
     8221 gctgaagacg gcgctcaaac acaggaccgg tcccaggcgc caggcgagtc gacaggcgcg
     8281 gagcgcgctg aagccgcgct tgacagtttc aaaattctgg ttgtggacga cgatcgctca
     8341 tcgctggaga tagtcaaacg cgtgctcgag gagcggcgcg cgaccgtatt caccgcggcc
     8401 agcgcccggc agggtctcga attattgcag cgctttcgcc cagatgtgct gatcagcgac
     8461 atcggcatgc cggaagaaga cggttaccgg tttctaaggc gtgtgcgggc actgggggcg
     8521 gatcgcggcg gcagcacgcc tgccatggcg cttaccgcgt ttgccagccc tgaagaccaa
     8581 caccgggcaa tgatatgtgg ctaccaagcc cacctgacca agcccatcga agccggccgg
     8641 ctgattgcca acgtggtccg cctgctgaac cagacccgcg aaaacggctc tgccaaatct
     8701 tgaattcgca cagcgggttt acgcgggccg tctcacaaag gccgggatgc gcttcactca
     8761 ggctgctcgc acacggtttc tgcacgcagc tcagtggcgc gctcgaccac ctccgacacg
     8821 gtcggcgtct ctgccggctc aagctcaaac gtcggcagaa cgtggttgtc ctgcaacatg
     8881 gccggaacga gcgcttcggc cacgaagtcg ataggggggt attgatacca gggcggcttg
     8941 atcgtgcagt gtgtctttag cgtctcgtag cccggcttgc gcaggatgat ctcgtaatcg
     9001 ccgtaccata ggaacgcgaa cttcaccggc gtcgcgccca cttcttcatc attgacggtc
     9061 accagggcgc cgggcggttc gctctgaatc ttgatagtgc gctcgacgca gcctgccagc
     9121 ggcgcgatca gcagtatcaa gcccatcagc aacgcgaggc cgggaacgtg cggccttcgt
     9181 gatcggataa agctcgcttt gaccgaactc atggtgtgat ctcgatttgc tcgatttgcc
     9241 tcttgtccag gtccaaaacg gcaatcgtgc agtggccggt tacccagccg cagcactcac
     9301 cggggttgag gaacaggcgg ccgtccttct cgtggacgcc aaattcatgc gtatggccgg
     9361 tgatgataac gtccgcctta cgaacgtcct ccgctcggca ccaatcaatg aaatgttgca
     9421 cgaggatggt ttttccgttg cgcgagatga agaggggccc gtcttgtatt tgcggcagca
     9481 ctttctgcag cccggcgcgt tcgccgtcat tattcccgaa ggtgacgtac agctccccgg
     9541 gccaggtagc caacacacgg gcggcaaaag gcgccacgat gtcacccggg tgaatgaggg
     9601 tgtgcaggcc gagttggctg aagcgttcaa gcgcggcgcg gatggcgggc aagcgatcgt
     9661 gcgtgtctga cagtacgcca atgcgcatgg gcaaactcgt gctaaaagcc gcgccgaaaa
     9721 aagcgtgcag tgccgatttt attcgctcgg cctgcgatcg tct
//
LOCUS       DAOSYS010000138         9591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6084_length_9591_cov_5.757131, whole genome shotgun sequence.
ACCESSION   DAOSYS010000138 DAOSYS010000000
VERSION     DAOSYS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6084_length_9591_cov_5.757131"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="manB"
                     /locus_tag="PLU99_09630"
     CDS             <1..517
                     /gene="manB"
                     /locus_tag="PLU99_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOO17357.1"
                     /translation="DVRCSRIVGEHVSKLGGVARRERVGHVYMKRALADHKGVFGGEV
                     SGHFYFQDFFNADSGLMAFIAVLNVLSRSGESLAGLIKPLDVYASSGERNFENEDKEG
                     TIRRLADTYAKGAIDYLDGITVQYPEWWFNVRPSNTEPLLRLNMEAVNQRLLEAKLAE
                     LTPLLGEPVEQ"
     gene            517..1767
                     /locus_tag="PLU99_09635"
     CDS             517..1767
                     /locus_tag="PLU99_09635"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO17358.1"
                     /translation="MTRLTAPRAGEIVRADLDCGIEFAADVLPGRNTAALVFRVLVGV
                     ADEPLELTGINGLVERTLSKGTKRFTGRSLADAFDALGASWASLSGRQSTIVRVLCLP
                     EFVVEAVGLVAEMLCRPTFPDEACEVAVELARQELRQMEDEPDELINVLINRLTLGPV
                     LGRYPGGEPHTLPRITPEKMREHWQRHYHGGRIQVAAAGPIDAESLARHVDAAFAELA
                     SPRREGREPAAFEFRPAREHQHKELEQEYVAITLRGVPKTDPQFPVEQMLLRVLAGGM
                     SGRLFTEVREKLGLVYWVGAWHEQLRGKGIIHLGASTTPERAHKTFDKLLEELGRIAE
                     DLTEAEVERARTGLIAHFQTEDDLTRSRAAGLSDDLFHFGRPVGLAAKLETLRAVTRD
                     DMLTYARRLPRDQLCIATLGPREL"
     gene            2091..3038
                     /locus_tag="PLU99_09640"
     CDS             2091..3038
                     /locus_tag="PLU99_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17359.1"
                     /translation="MDARRDQAAENDSPDLARQMDEVAAGISSITEGWGVLAERLAAF
                     QAALQLRLEELNQRETRLSDREAGLTDREASAAAREADLERQSKELADAKQELEAGGG
                     EIRAWQQELEQMQSALEARGAELEAAEARLAAREEAVRKFQATLAGLMQAFGTAVPSE
                     LIQAQSESTLSGENPAPAASEEPAPDLQEPPVVDEAEPDLVSQVLEASDDATALPESD
                     DAVAEAVPEPGVEEETQPEQAEEQPAEAEHEGRDSAPEGDGAPAAEDLDEDTRQKLKV
                     LRRLTGGRVSDEKLLERISQERQVSTDGPSAGKKRHWWG"
     gene            3099..4031
                     /locus_tag="PLU99_09645"
     CDS             3099..4031
                     /locus_tag="PLU99_09645"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOO17360.1"
                     /translation="MKASAVLNSPLASDASAGPLPSFFVIGARKCGTTTLDQYMRQHP
                     QICLPRGKKELNFFGDQAHRGLAWYRAHFAHAGADQLCGEVAPMYSIEQDPPCARRIA
                     ELVPQARLIYIVRHPVERAYSDFCEQIKTARALGRNYAWFKSFDDFLDANPRAVRASE
                     YIRTIEEYQAYFPPEALLTVLFDDLKADPTALLRRIYAHIGVDSGAAAATDRPIRANA
                     GDEYLQWQTRLRLTRTLRALPGVATAAQFIPRSWCNRAYTLLERSRFGQAARAALAPP
                     PLSAQTRQRLLERFRAHNRALAAYLGRDLAHWDR"
     gene            complement(4071..6347)
                     /locus_tag="PLU99_09650"
     CDS             complement(4071..6347)
                     /locus_tag="PLU99_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17361.1"
                     /translation="MCRYLCALTIVTVCCAVLGRAQTADALDDALALVGLQRADLGWA
                     PKGWWPRYPADIPYKLRSFDALFNEPLDTVAFSRGLGETAWDKLDPAKLDKENGRGAR
                     SLFEAVQRLGIDPKFGGFRGYTANVLAPELPLDQALLKLFEAAHRTTRPNTFGMELPY
                     PRIVDQLPEQVKVLPEGVSPILGQLVLNIIDAHYWAQLAFRRADPHARLVAATRYNTG
                     EEMIDAYDYCPEFDDLAQTWDEASLWYAGQKCVDALDLARIRLQAWKAGLAEPPPPFA
                     FDCETPWGWVRIRGSGNDFIDAAGAVRTLLIVDLGGNDTYLGPVAASSASYPLSLLLD
                     LDGEDEYSAGAGPAAEADDSTNPALRGAPGPAQGAGLCGVGVLIDAAGDDQYSAERYA
                     QGVGQFGLGVLADLAGNDRYGVRYSGQGCGYFGVGLLLDVAGIDNYNIYCDGQGLGGV
                     AGVGVLADRSGDDRYIAERKHSVTGRPSYHSPNLDVGVSNVQGCGMGRRGDGADGHAW
                     AGGLGALLDGEGNDYYYAGNWCMGTGYWFGVGVLHDRGGDDEYHGAVYSQGTGAHFCI
                     GVCVDEAGNDQHLAEENSHSSLAWAHDFTIGLLLDVAGDDVYRVDGSGLSYSINRSVS
                     MLLDLAGDDKYQTDLARREQEKAGLCPGYAWNPEGFRSRGIAQYFADTTSLGLFLDIG
                     GHDEYPVPPATQPADMTERTDVAASARAGNDRHWLDPPDSPNWADRNFSVGVDRAAGQ
                     VDFTPRPVKPPSNPRSAP"
     gene            complement(6593..6820)
                     /locus_tag="PLU99_09655"
     CDS             complement(6593..6820)
                     /locus_tag="PLU99_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOO17362.1"
                     /translation="MAKYKVEVDRNLCVGDGACVNEAPGTFEMDDESIATVTNPDGNT
                     PEEILAAAQSCPVDAIILTDAATGEKVWPQG"
     gene            7112..8914
                     /locus_tag="PLU99_09660"
     CDS             7112..8914
                     /locus_tag="PLU99_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOO17363.1"
                     /translation="MNDQRFRYPIWARGDLDGFFGLMVDNLAQVLLIITLCTTVAHLP
                     ESLIYQRILPGVAISLLVGNIFYGVQGHYSARKYRNSTCTALPYGINTPSVVAYVLFI
                     MGPVYEQHKAALGEEGAGVLAWQAGLLACLVSGVIEATGALYAEKLRRVTPRAALLGV
                     LAAVGIAFIAAEFAFRIYTAPLVGVLPLAFLLLAYFARYRFPLGIPGGFLAILFGTLI
                     AWAGTAWGFYDFGGVRMSGEDLRASLDHVRWIPPVFIGNELWDLLTRQPQLVLMFLTV
                     SIPMGVINVLGSLQNIESAEAGGDRFHTGLSLGVNGIGTLAAGLFGSCFPTTIYIGHP
                     GWKALGARSGYSILNGLFFTAMFLFGLGPLLAALVPIEAGSAIVMYIGIIITAQAFQV
                     TKREHAPAVAVALFPALAAILVMTLANYFVTAGAAASLADLMQRWSIDPVLQKSLYLP
                     GLLALTGANSGWIVVTLILAAIGAALIDHKYKAATIWAAAGALLTLIGAMHAYRLEGN
                     VIREFFIWQGPAGATVTYSAFPIAIGYALAALLFGLAAWRESSRHNSAPSCKTELPVA
                     EMAPAVSEVNEVQTAEVYQVQPVEARPPEPSANA"
     gene            8937..>9591
                     /locus_tag="PLU99_09665"
     CDS             8937..>9591
                     /locus_tag="PLU99_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17364.1"
                     /translation="MSLHDRESCHVACLFVVAVVFTVRAQAADPPRPSLRQPVDYVRW
                     VNEEFSRGITENAAEFYQRALSEFSEDDSLEKFIAEADASTWTPEQRRQRSAWVQKNA
                     TCLEHFAKAARMDACYFPLPRESGALVSVMLPELSTFRAIAKLMTVRARLRLLEGNVD
                     GATEDVALLLRAGRHLQDQPFLIQYLVGLSINGLAYRVLLDMPRLAPRAPDYEKVLSK
                     "
BASE COUNT         1686 a         3015 c         3187 g         1703 t
ORIGIN      
        1 cgacgttcgt tgcagccgga tcgtgggcga gcacgtgagc aagctggggg gcgtggcgcg
       61 gcgcgagcgg gtggggcacg tgtacatgaa gcgcgcgttg gccgatcaca agggcgtgtt
      121 cggtggggag gtcagcgggc acttttactt tcaggacttt ttcaacgctg actcggggct
      181 gatggctttt atcgcggtgc tgaatgtgct ttcgcgcagc ggcgagtcgc tggcgggttt
      241 gatcaaaccg ttggacgtct acgccagcag cggtgagcgg aacttcgaaa acgaggacaa
      301 ggaagggacc attcggcgcc tggccgacac atacgccaag ggcgcgatcg attacctgga
      361 cggtattacg gtccagtacc cggagtggtg gttcaacgtg cggccgtcga ataccgagcc
      421 gctgttgcgg ctgaatatgg aggccgtcaa tcagcggctg ctggaggcga aactggccga
      481 gctgacgccg ctattgggcg agccggtgga gcaatgatga cgcgattgac ggcgccgcgg
      541 gcgggcgaga tcgtccgcgc cgacttggat tgcgggattg aattcgcggc cgacgttctg
      601 ccggggcgca acacggcggc gttggtgttc cgggttctgg tgggcgtagc cgacgagccg
      661 ctggagctga cgggcatcaa cgggctggtt gagcggacgc tgtcgaaggg aacgaagcgt
      721 ttcaccggac gtagcctggc ggacgcgttc gacgcactgg gggcgtcgtg ggcaagcctg
      781 agcgggcggc agagcacgat cgtgcgcgtg ctgtgtctgc cggaattcgt ggttgaagcg
      841 gtggggctgg tcgcggagat gctctgccga ccgacatttc cggatgaggc gtgcgaggtg
      901 gcggtggaac tggcgcgcca ggaattgcgg cagatggagg acgagccgga tgagttgatc
      961 aatgtgctga ttaaccggct cacgctgggg ccggtgctgg gccgttatcc tgggggcgag
     1021 ccacatacgc tgccgcgtat cacgccggag aagatgcgtg agcactggca gcgtcactat
     1081 cacggcgggc ggatacaagt ggcggcggct ggtccgattg atgcggaaag tctggcgcgt
     1141 cacgtggacg cggccttcgc ggagctggcc agcccgcggc gggaggggcg tgagccggcg
     1201 gcgttcgaat tccggccggc gcgcgagcac cagcataagg agctggagca ggaatacgtg
     1261 gcgattacgc tgcggggagt gccgaaaacc gatccgcaat tcccggtcga gcagatgttg
     1321 ttgcgcgtgc tggcgggcgg gatgagcgga cggttgttca ccgaagtgcg cgagaagctc
     1381 gggctggtgt attgggtggg ggcgtggcac gagcaattgc gcggcaaagg cataattcac
     1441 ctcggggcca gcaccacccc ggagcgggcg cacaagacgt tcgacaagct gctggaggaa
     1501 ctggggcgca tcgcggaaga cctcacggaa gctgaagtag aacgggcgcg caccggcctg
     1561 atcgcgcatt tccagaccga ggacgacctc acccgttccc gcgccgcggg tctcagtgat
     1621 gatcttttcc atttcggccg gccggtcggg ctggccgcca agctggaaac cctgcgggcc
     1681 gtgacgcgtg acgacatgtt gacttacgcg cggcggctgc cacgtgacca gctctgcatt
     1741 gcgacgcttg ggccccgtga gctgtagtgc tgggcggcaa tcgagtcgcc gagcgcaacc
     1801 cgtgcacact gccgacggtc tcataatcgg gttcgatcgt gcggggctgg ctggttcggg
     1861 accggcggga ttatcggcag cgcggcttgg aagcgtcgcg cgccctttgg gaatcaagtt
     1921 cttctccgat tgcctcgatt cagtaaggga ttgacgcgtt gttgcggctt cggcggcgaa
     1981 cgcgcgctct gaagcgcgcc cggtttggcc ggccaggcgg gcaagttgcg cgtgggccag
     2041 cggccacgcg cgctccgata gccggacggc gcctgcggaa aggctaatca atggatgccc
     2101 gacgggacca ggccgccgag aacgacagcc cggacctcgc ccggcagatg gacgaggtgg
     2161 ccgcgggcat ttcgtcgatt accgagggtt ggggcgtgtt ggccgagaga ttggccgcct
     2221 ttcaagccgc cctgcagctc cggttggaag aattgaacca acgcgaaacg cggctttcgg
     2281 atcgcgaagc cggcctgacg gatcgcgagg caagcgccgc ggcgcgcgaa gcagatctgg
     2341 aacgccagag caaagaactg gccgatgcca agcaagaact ggaagcgggc gggggcgaaa
     2401 tccgcgcctg gcaacaagaa ctggagcaaa tgcaatcggc tctggaagcg cgcggcgcgg
     2461 aactggaggc cgcagaggcc aggctcgcgg cccgcgagga ggcggtgcgc aagttccaag
     2521 ctaccctggc cgggctgatg caggcgttcg gcacggctgt cccgagcgag ctgatccaag
     2581 cgcaatctga gtcaaccctc tcaggcgaga atccggcgcc ggcggcatcg gaggaaccgg
     2641 ccccggatct tcaggagcct cccgtcgtgg acgaagcgga gccggacctc gtgtcgcagg
     2701 tcttggaagc atcggacgac gctaccgctc tgccagaatc cgatgatgcc gtagcagagg
     2761 cggtcccaga accgggcgtg gaagaggaga cccaaccgga gcaggccgag gagcagccgg
     2821 cggaggcgga gcacgaagga cgcgactcgg ctccggaagg cgacggtgcg cccgcggccg
     2881 aagacttgga cgaagataca cgccaaaagt tgaaagtcct gcgccggctg acaggcggcc
     2941 gcgtatcgga cgagaagctc ctggagcgca tctcgcaaga gcgccaggtc tccacggacg
     3001 gtcccagcgc gggcaagaag cggcactggt ggggctgatc cggccgccgt gctatccagc
     3061 cgattaatcg gcggggcggt gcgacacgga ggaaacggtt gaaagcttcc gctgtcttga
     3121 actcgccgct cgcaagcgat gcttcggccg ggccgctgcc gtcgttcttt gtcatagggg
     3181 cgcgcaagtg cggcaccacc acgcttgacc agtacatgcg ccagcaccca cagatttgtc
     3241 tgccgcgcgg caaaaaggag ctcaacttct tcggcgacca ggctcaccgc ggtttggcct
     3301 ggtaccgcgc gcattttgcg catgccggtg cggaccagct ttgcggcgaa gtggcgccca
     3361 tgtattccat cgagcaggat ccaccgtgcg cgcggcggat cgccgaactc gtcccgcaag
     3421 cgcggctgat ctacattgtg cggcatccgg ttgaacgcgc gtactcagac ttttgcgaac
     3481 agatcaaaac cgcccgtgcg ctcgggcgaa actacgcctg gttcaaatcg ttcgacgact
     3541 ttctcgacgc aaacccgcgg gccgttcggg ccagcgagta catccgcact atcgaggagt
     3601 accaagctta cttccccccc gaggccttgc tgacggtgct gttcgatgat ctgaaggccg
     3661 accccaccgc gcttttgcgc cgcatctacg cgcacattgg tgtggactca ggcgcagcgg
     3721 ccgcgacgga ccggcccatt cgagccaacg ccggggacga gtacttgcaa tggcaaacgc
     3781 gactgcggct cacgcggacg ctacgcgccc ttccgggtgt tgcgaccgcg gcacagttca
     3841 tcccgcgttc ctggtgcaat cgggcctaca cgctcctgga gcgttcgcgc ttcggccagg
     3901 ccgcacgcgc tgcgcttgcc ccaccgccgt tgtccgctca gacccggcag agactgctcg
     3961 agcgcttccg ggcgcacaat cgcgccctgg ccgcctacct gggacgagac ctcgcgcatt
     4021 gggatcgata gccggccgcc agcgcgctcg ccctcggccc gccaggcatc ctacggcgcc
     4081 gatcgtgggt tgctcggcgg cttcaccggg cgcggcgtga aatcgacctg tcccgctgcc
     4141 cgatccacgc cgacgctgaa attgcggtcg gcccagttcg gcgaatccgg tggatctagc
     4201 caatggcggt cattgccggc ccttgcgctt gcggcaacat cggtccgctc ggtcatatca
     4261 gcaggctgtg tggccggcgg aacggggtac tcatcgtgac caccgatgtc gaggaacagg
     4321 ccaagcgagg tagtgtccgc aaagtactgg gcgatgccgc gggaccggaa gccctccgga
     4381 ttccaagcat agcctggaca caggccggcc ttctcctgtt cgcgccgcgc cagatcggtc
     4441 tgatacttgt catctccggc caggtctaac agcatcgaca cgctgcgatt tatggagtac
     4501 gacaagccgg acccgtccac tctgtacacg tcgtcgccgg cgacatcgag caacaggccg
     4561 atcgtgaaat cgtgcgccca cgctaagctg ctatgcgaat tttcttcggc caagtgctgg
     4621 tcgttgcccg cctcgtcgac gcacacgccg atgcagaagt gggcaccggt gccctgcgag
     4681 taaaccgcgc cgtgatactc atcgtcgccg ccgcgatcgt gcaggacgcc gactccgaac
     4741 caataccccg tacccatgca ccaattcccg gcgtagtagt aatcgttgcc ctcaccatcg
     4801 agcagcgcgc ccagtccgcc ggcccatgcg tggccgtcag cgccgtcgcc acggcggccc
     4861 atgccgcagc cctgcacgtt cgagacgccc acatccaggt ttggcgaatg gtacgacggc
     4921 cgaccggtga cgctgtgttt gcgctccgcg atgtagcggt cgtcgccgct gcgatccgcg
     4981 agcacgccga cgcccgccac cccgcccagt ccctggccgt cgcagtaaat gttgtagttg
     5041 tcgatgcccg ccacatctaa cagtagacca acgccgaaat agccgcagcc ctgacctgaa
     5101 tagcgtacgc cgtaccggtc gttgcccgcc aagtcggcga ggacccccag cccgaactgg
     5161 cccacgccct gcgcgtaccg ctcggccgag tactggtcat cgccggccgc gtcgatcaag
     5221 acgccgactc cgcacaggcc ggcgccctgt gccggaccgg gtgcgccgcg taaggccgga
     5281 ttggtcgagt cgtcggcctc tgcagcaggc cctgcaccgg ccgagtattc atcctccccg
     5341 tcgagatcga gcaggaggct gagcggataa gacgcgcttg aggcggcgac cgggcccaag
     5401 taggtgtcat tgccgcccag atccactatt agcagcgtcc gcacggcgcc tgccgcgtcg
     5461 atgaagtcgt tacctgagcc gcgaatccgc acccagcccc agggcgtctc acagtcgaat
     5521 gcgaagggcg gtggcggttc ggccagtccg gctttccagg cttgcagcct gatgcgcgcc
     5581 aggtcaagcg catccacgca cttctgcccc gcgtaccaca gtgacgcctc gtcccaggtt
     5641 tgggccaggt cgtcgaactc cgggcaatag tcgtacgcgt cgatcatctc ctcgccggtg
     5701 ttgtagcgcg tcgccgctac caggcgcgca tgcggatccg cgcggcggaa ggccagttgc
     5761 gcccagtagt gcgcgtcgat gatgttcagc acgagttgcc cgagaattgg gctgaccccc
     5821 tccggcaaaa ctttcacctg ttcgggaagt tgatcgacga tgcgcggata gggcagctcc
     5881 atgccgaatg tattgggccg cgtcgtgcga tgcgcggctt cgaacagttt cagcagagcc
     5941 tgatcgagtg gcagctccgg cgccaaaacg tttgccgtgt agccccggaa cccgccgaat
     6001 ttcgggtcga tgcccagccg ctggaccgcc tcgaacaacg accgtgcgcc gcggccgttt
     6061 tccttatcga gtttggccgg gtcgagctta tcccacgctg tctcgcccaa gccgcgactg
     6121 aacgcgaccg tatccagcgg ctcgttgaag agtgcgtcaa acgagcgcag cttgtacgga
     6181 atatccgccg gatagcgcgg ccaccagcct tttggggccc aacccaggtc cgcgcgttgc
     6241 aggccgacca gcgcgagcgc gtcatcgagc gcatcagcag tttgggcgcg ccccagaact
     6301 gcgcaacaca cggtcacgat cgtcaaagca cacaggtatc tgcacatggc tgctccgctc
     6361 tccaccccgc gggaacgaag catactgggc gcacagccgc gctgcaaagt ccgcggcgct
     6421 cggccccgtc ggccgtggag acggcgaggt ttatcgagat ggaaacccgc ccttcagcga
     6481 cagtcaggcc tcagtcctgg gtgcaaaagc tgagagccag caggcgcgcc gcgacctgcc
     6541 cgctacaaac ggcgagcggg gcgcggcgcg accgtgaaaa ggcacctaag ctttagccct
     6601 gcggccagac cttctcgccg gtggccgcat cggtcaggat gatcgcgtcg accgggcagg
     6661 attgagcagc ggcaagaatt tcctcgggcg tgttgccgtc ggggttggtg acggtcgcga
     6721 tggactcgtc gtccatctcg aaggtgcccg gggcctcgtt gacgcacgcg ccatcgccga
     6781 cgcagagatt acgatcgact tcgactttgt acttcgccat gttttactcc agtcgcccga
     6841 tagcccctgc cagggactcc ccaggcagcc gggcgcatca tgtggctgat acactttccg
     6901 ttccagtcgg cgcttaaatc aggccgggcg gccgcagcac cgactcgccg gaacgcccgg
     6961 caatgcggaa acagataaat tagccaactc ggagcaggtt tcgcaagccc cccgcgccgc
     7021 ggtcatttgc cccgccttga gcgccggccg gccgcgccat agcatgccgt cgcggcttga
     7081 acattcttcc tgaaaaaccg gacatttgaa catgaacgac cagcgatttc gttacccgat
     7141 ttgggcgcgc ggcgatctgg acggcttttt cggcctgatg gtcgacaacc tggcccaggt
     7201 gctgctcatc atcaccttgt gcacgaccgt agcgcatctg ccggagtcgc tgatctacca
     7261 gcgcatcctg ccgggcgtcg ccatcagcct gctggtcggc aacatcttct acggggtcca
     7321 gggtcactac agtgcccgca agtaccggaa ttcgacgtgc accgctctgc cttacggaat
     7381 caacacgccg tcagtggtgg cgtacgtgct cttcatcatg ggacccgttt acgaacagca
     7441 caaagcggcc ctgggcgaag agggcgcggg cgttctcgcc tggcaggccg gcttgttggc
     7501 ctgcctggtc agcggggtca ttgaggccac cggcgcccta tatgcggaaa aactccgccg
     7561 cgtcacccca cgtgcggccc tgctgggagt actggccgcg gtcggcatcg cgttcattgc
     7621 ggccgaattc gcgttccgca tttataccgc tccgctggtg ggcgtcctgc cgctggcgtt
     7681 tctgctgctg gcgtatttcg cgcgttaccg ctttccgctc ggaataccgg gcggattcct
     7741 ggctatcctg ttcgggacgc tgatcgcctg ggccggcacg gcctggggct tctacgattt
     7801 cggcggcgtg cgcatgtccg gcgaagactt gcgggcctcg ctggaccacg tccgctggat
     7861 accaccggtg ttcattggga acgagctgtg ggatttgctg acgcggcagc cgcaactcgt
     7921 gctcatgttc ctgaccgtct cgattccgat gggtgtcatc aatgtgctcg ggtcgttgca
     7981 gaatattgaa tcggccgagg cggggggcga ccgctttcac accgggttat cactgggtgt
     8041 gaacggcatc ggcacgctgg cggccgggct attcgggtcg tgctttccaa cgacgattta
     8101 catcggtcat ccgggctgga aggccctggg cgcccgctca ggctactcca tcctgaacgg
     8161 cctgttcttc accgcgatgt tcctgttcgg gttgggaccg ctgctggcgg cgctggtgcc
     8221 gatcgaggcg ggcagcgcca tcgtgatgta catcggcatc atcatcacgg cccaggcctt
     8281 ccaggtcacc aagcgcgagc acgccccggc ggtggccgtg gcgctgtttc ccgccttggc
     8341 ggccattctg gtcatgacgc tggcgaacta cttcgttacg gccggggctg ccgcctcgct
     8401 ggcagacctg atgcagaggt ggtccatcga tccggtgttg cagaagtctc tctacctgcc
     8461 cgggctgctg gcgctgaccg gggcgaactc cggctggatc gtggtaacgt tgatactggc
     8521 cgccattgga gcggcgctga tcgaccacaa gtacaaagcc gcgacaattt gggcggccgc
     8581 cggcgcgctg ctgacgctaa ttggcgccat gcacgcctac cggctggaag gcaacgtcat
     8641 tcgcgaattc ttcatctggc aaggcccggc gggcgcaacc gtgacgtaca gtgcctttcc
     8701 gatcgccatc ggttacgcgc tggccgcgct gttgtttgga ttggcggcct ggcgcgaaag
     8761 ctcgcggcac aattcggcgc cgtcctgcaa gacagagctg ccggtggcgg aaatggcgcc
     8821 ggccgtttcc gaggtaaacg aagtgcaaac tgccgaggta taccaagtgc aacctgtgga
     8881 ggccaggccg cctgagccgt cggcaaatgc ctgaacacgt gaaacagcag ggaggagtga
     8941 gcttacatga cagagagagc tgccacgttg cgtgcctctt cgtcgtcgcc gtcgtgttta
     9001 ctgtccgcgc acaggccgct gatccgccgc ggcccagttt gaggcagccg gtcgattatg
     9061 tgcgctgggt gaacgaggag ttttcccgcg gcattactga aaacgcggcc gaattttacc
     9121 agcgggcact ttccgagttc tcagaagacg acagcctcga aaagttcatt gccgaagccg
     9181 acgcaagcac gtggacaccc gagcaacggc ggcaacggag cgcgtgggta cagaagaacg
     9241 cgacctgttt ggagcacttc gcgaaagcgg ctcgtatgga tgcgtgctac tttccgctcc
     9301 cgcgcgaatc cggcgcgctg gtttcagtga tgctccccga acttagcacc tttcgcgcca
     9361 ttgccaagct gatgacggtt cgggcacggc tgcgactgct cgaagggaac gttgatggag
     9421 cgactgaaga cgtggcgctg ctgctccgcg ccggccgcca cttgcaggac cagccctttt
     9481 tgattcagta cctcgtcggg ttgagcatca atggactggc ttatcgtgtg ctgttggata
     9541 tgccccggct ggctccccgc gcgccggact atgagaaagt actctcgaag c
//
LOCUS       DAOSYS010000139         9584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6089_length_9584_cov_4.588414, whole genome shotgun sequence.
ACCESSION   DAOSYS010000139 DAOSYS010000000
VERSION     DAOSYS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9584
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6089_length_9584_cov_4.588414"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLU99_09670"
     CDS             <1..875
                     /locus_tag="PLU99_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17365.1"
                     /translation="ESLRFAAGAAMATLVLAIPIFLGIPWLYPWYSEPPAADTFRASY
                     LNPAAFVGRGAVYLVFWAILGAVLARTGEPDERRTRKWAGPVLVFYLLTLTFAAIDWV
                     GSIVQHWYSTVFGFYLIVGQALSALALLVLLAARRPDQPETQTRHDWGNLLLTFVVLY
                     AYMAYSQFLIIWSGNLPHEITFYVQRVRGAWGALAVVLIILGFLLPFGALLARNAKRR
                     AQALAAIAAVVLFARLLDSAWMVLPGLSAGQLLGLLAYFLLLPGLVWVWLLAAGWLHA
                     WVPASAELRREARP"
     gene            872..1366
                     /locus_tag="PLU99_09675"
     CDS             872..1366
                     /locus_tag="PLU99_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17366.1"
                     /translation="MSISPAGSEHPAPYGHEPESASVRGTVAAIISLAVLIALIMTVV
                     YWFMGEVRQHRMVPPEYTRPRTQVQQPSLSVQDWTDPGQALEQLRQREYTRLHNYEWI
                     DRAAGRVQIPITRAMELLAAGAKLPSTPSTAPAGAPAPPPATESATAPAATGPSEGSG
                     GAAQ"
     gene            1363..2385
                     /gene="coxB"
                     /locus_tag="PLU99_09680"
     CDS             1363..2385
                     /gene="coxB"
                     /locus_tag="PLU99_09680"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOO17367.1"
                     /translation="MNLPLAQFWSTGMPQRASEGAERVDALLLGLLGVSAFFILLILI
                     LIVRFAIKYRAGSSADRANPVRKTWGWELTWIAIPSLLMVGMFVWAGILYLHMQEPPA
                     DARTIYVVGKQWMWKVQHPEGPEEINELHVPAGEPVKLVLSSQDVIHSFFIPVFRIKQ
                     DVLPGRFTTLWFTATKPGVYPFYCAEYCGTEHSMMHGQMVVMTPGEFAAWLDTAVRDA
                     EPVPGTPGSPAAPLALGEQGAFYRLGCVACHTRQSAVLAPRLDGIFGRPVRLANGQTV
                     IADEDYIRESIMYPNAKISAGYPAPSLMPTYAGQVSPADLRDLVEFIKALRDGWPSED
                     HTHGEH"
     gene            2372..3994
                     /locus_tag="PLU99_09685"
     CDS             2372..3994
                     /locus_tag="PLU99_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOO17368.1"
                     /translation="MANIEGAPAVTDYLQTGWRARDWFLSTDHKRVAILYLLSITFFF
                     FIGGLAATLIRVELLTPPSNLVSKDVFNRLFTMHGVIMVWFFLIPSIPTTIGNFVIPL
                     MIGARDLAFPKLNLFSWYLFSAGGLFTLGAIILGGVDTGWTFYTPYSSFYAHSAVVLV
                     ILGIVVAGFGSILTGLNFIVTVHTMRAPGMGWFQLPLFIWSHYATSLILVLATPILAI
                     TLLLVGIERVWQYGIFEPSLGGDPLLFQHLFWFYSHPAVYIMVLPAMGVVSEIVPCFA
                     RKHIFGYHFVAFASLAIATYGFLVWGHHMFVSGQSPYASLIFSALSFLVAVPSAIKVF
                     NWTATLHKGYIHLRTPMLYALGFIGLFVIGGLTGLFLASTALDVHVHDTYFVVSHFHY
                     IMVGGAVMGYLGAIHFWWPKMSGRRYPEGWGIFAAILLFVGFNLTFFPQFLLGYGGMV
                     RRIAEYAPGFQVLNVMSTAGASILGVGYVLPLAYLLWTLRRPPDAGPNPWFATGLEWQ
                     TSSPPPKHNFVAAPQVGEPYDYAEMPRDIPWS"
     gene            3985..4641
                     /locus_tag="PLU99_09690"
     CDS             3985..4641
                     /locus_tag="PLU99_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HOO17369.1"
                     /translation="MELTKVETPVEMQFDDPAQQREASALGMWAFLASEVLFFGGVFT
                     GYSIYRWKYPQAFAEASHHLYEWIGAVNTAILLTSSLFIALAVQALHTERPRRAWRLI
                     IATLICGLAFLSLKAVEYWMDYQEGLVPGVRFDASKFAEPGPATLFFTFYFSMTGLHA
                     AHVISGLLVLTVIGVTLRRAADPGKYANSLENGALFWHLVDIIWIFLFPLLYLIGGPK
                     "
     gene            4638..4913
                     /locus_tag="PLU99_09695"
     CDS             4638..4913
                     /locus_tag="PLU99_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOO17370.1"
                     /translation="MTGGPRTATLYWTAVTLGLLLGGTVVATEFLGRAGPTVNLVIAA
                     VKAALVAVVFMHLRWSSPLQRLFAGAAFFWLALLFALTFADYLTRRA"
     gene            complement(4892..5599)
                     /locus_tag="PLU99_09700"
     CDS             complement(4892..5599)
                     /locus_tag="PLU99_09700"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOO17371.1"
                     /translation="MHRPRRLKAPVTREKLIEAAGEIFAEKGLHGTSIRDITQRAGAN
                     VASVNYHFHDKFELYAQVLRRAHEGIVAAMSRPLTAKTPEGRFRELITAMLKSALDPS
                     RPKWQTRVLGRELFQPTPVLDLMDDLVRPTAHRLSQTMGEIRPDLSFQQLTLAAAGVV
                     ARAVFHVYHGHLVQRMFPAAPEATPDELIEHVVEFSLAALRGLQAPPACGQNHRPRSC
                     RATVKRRANAAHARRVR"
     gene            5801..7027
                     /locus_tag="PLU99_09705"
     CDS             5801..7027
                     /locus_tag="PLU99_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO17372.1"
                     /translation="MTIRNRRPLGGRMSRLVRGSLFLAAPAALAALIGCNSSPQPRPQ
                     TEAPPPEVAVVTLEAQPVSLTTELPGRTSAYRIAEVRPRVGGIIEKRLFAEGTDVEAG
                     QQLYQIDSRRFVAQLDHADANVKRARAELARADYELQRLTKLRQSDAAADKEYQDALF
                     AQQAAEAALDLALSEQRLAALNLEWTVVVAPISGRIGYSSVTEGALVTAEQPTPLTTI
                     QQIDEIYVDVTQSSADLLRLRRQWETGAISSGDGIVRLLLEDGTPYPLDGKLQFRDIT
                     VDPTTGSYTLRIVFPNPDHLLLPGMFVRAVVHEGTAPQALLVPQQGVSRNPKGEPVAL
                     LVDEANKVQQRKLTLNRPIGNRWLISAGLSPGDRLIVEGALRVRPGDLVKAVPFEEGK
                     QKVAVGEAKNVTGGSE"
     gene            7040..>9584
                     /locus_tag="PLU99_09710"
     CDS             7040..>9584
                     /locus_tag="PLU99_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO17373.1"
                     /translation="MLSNFFLDRPVFAWVIAIIMMLAGGLAIYNLPVSQYPPIAPPSI
                     AIDAFYPGAAAETVENSVVQIIEQKMTGFDKLLYMTAISEASGGGRIELTFAPGTDPD
                     LAWAQVQNKLQLAMASLPDVVQRQGVKVSKSTRNWLLIVGLVSNDDSVDDIDLRDYAA
                     ANIEKVLARVPGVGEVEVFGSQYAMRIWLNPDKLTNYKLTIDDVIAALRAYNVEISAG
                     QFGAAPAVEGQRLNASIIVQNLLKTPEEFAEVPLRTNPDGSVVRVRDVGRTELGTERY
                     EINALYNGRPCAALAVRQAAGANALETARAIKATMDELSQYFPPGMGVIYPYDTTPFV
                     RVAINEVVKTLLEAIVLVFLVMYLFLGNIRATLVPTIAVPVVILGTFAVLGALGYSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERVMHEEGLSPREATRKSMREITSALVGIGTVL
                     AAVFGPMAFFGGSTGVIYRQFSATIIAAMLLSVVVALILSPVLCAGLLKPVAHGHAPA
                     DTGFVLFRPFFRIFDRVFFWIRDRYRDMVGRILGRKIRYLLVYVVLVAALGYLFQRMP
                     TSYLPEEDQGMMFVQAMLPVGSTLEQTEAVMNDVRKYFEEHETEAVASCMTISGRAFS
                     GGGQNVGMAFIRLQDWELRQRPDLKVKAVIGRAMQAFSRIRNAQVYAFAPPAVVELGT
                     ASGFDFQLQDRGGLGHEALMAARNQLLSMAAQDPRLIRVRPNGLEDVPQYRIDVDWER
                     AGVLGVPISAIHDTLAAAFGSAYVNDFIQGGRVKRVYVQADAPYRMLPSDLERMYVRN
                     QAGLMTPFSAFASGRWSYGSPRLERYNAFPSLNILGEAAPGRSSGEAMQA"
BASE COUNT         1623 a         2994 c         3040 g         1927 t
ORIGIN      
        1 acgagtcact gcgcttcgcg gccggagcgg cgatggcgac cctggtcttg gccattccga
       61 tcttcctcgg cataccctgg ttgtacccgt ggtactcgga gccgcccgcc gccgacactt
      121 ttcgcgcctc gtatctgaac cctgcggcct tcgtcgggcg cggcgcagtc tacctggtat
      181 tctgggccat attgggcgcc gtactggcgc gcacgggaga acccgacgaa aggcgcacca
      241 ggaagtgggc cggtcccgtc cttgtcttct acctactcac actcacgttc gccgccatcg
      301 actgggtcgg ctcaattgtc cagcactggt attcgaccgt cttcgggttc tacttgattg
      361 tcgggcaagc actgtcagca ctcgccctgc tggtgctgct ggccgcacgg cggccggatc
      421 agccggaaac gcagacgcgc catgactggg gcaacctgct gctgaccttc gtcgtcttgt
      481 acgcctacat ggcgtactcg cagttcctca tcatttggag tggaaatctg ccgcacgaaa
      541 taacattcta cgtacagcgc gtgcgaggcg cgtggggcgc gcttgcggta gtgttgatta
      601 ttcttggatt cctgttgccg ttcggagcac tgctggcgcg gaatgccaag cgcagggctc
      661 aggcgctggc cgccatcgcc gccgttgtgt tgttcgcccg gctcctcgac agcgcctgga
      721 tggtcctgcc tgggctgtcg gcggggcaac tgctggggct gctcgcatac tttctgctgc
      781 tgcccgggct ggtctgggtc tggctcctgg ccgccggctg gctgcatgcg tgggtgccgg
      841 cttccgccga actgcgccgc gaggcccgac catgagcatt tcgccggccg gttctgaaca
      901 tcctgcgccg tacgggcacg agcccgaaag cgccagcgtg cgcggaacgg tcgcggcgat
      961 tatcagcttg gctgtcctga tcgcgctaat tatgactgtg gtttactggt tcatgggtga
     1021 agtgcgccag caccgcatgg tccctcccga gtacactcgg ccccgaacac aagttcagca
     1081 gccttcgctt tcagttcagg actggacgga cccgggccag gcgctggaac aactgcgaca
     1141 gcgcgagtac acccgccttc acaattacga gtggattgac cgtgcggccg gccgcgtaca
     1201 gattccgatc acacgcgcca tggaactcct ggcggccggc gccaagttgc ccagcacgcc
     1261 gtctaccgca ccggccggcg cgcccgcccc tccaccggcc accgaatcgg ccactgctcc
     1321 ggccgcaacc ggcccgtccg aaggttcagg aggtgcggcg caatgaactt gccgttggcc
     1381 cagttctgga gcaccggcat gccgcagcgc gcctcggaag gtgcagagcg cgtagacgca
     1441 ttgctactgg gtctgctcgg cgtttcagca tttttcatcc tgctcatcct gattttgata
     1501 gtgcgcttcg caatcaagta ccgcgccggt tcgtcggcgg accgcgcgaa cccggtgcgc
     1561 aagacctggg gctgggaact cacctggatc gcgattccct cactgctgat ggtcggcatg
     1621 ttcgtatggg cgggcatact ttacctccac atgcaggaac cgcccgcaga cgcgcgcact
     1681 atctacgtcg tcggaaagca gtggatgtgg aaagttcagc atcccgaagg acccgaggaa
     1741 atcaatgagc tgcacgtgcc agccggcgaa ccggtcaagc tcgttctaag ctcgcaggat
     1801 gtaatccaca gcttcttcat tccggtcttt cgcatcaagc aggacgtctt gccgggacgt
     1861 tttaccacgc tctggttcac cgcgaccaag cccggcgtct atccgttcta ttgcgccgag
     1921 tactgcggga cggagcactc catgatgcac ggccaaatgg tcgtcatgac ccccggcgaa
     1981 ttcgccgcct ggctggacac cgccgtccgt gacgccgaac cggtgcccgg cacacccggc
     2041 tcgcccgcgg cgccgctcgc gctcggtgag cagggcgcct tctaccgcct gggttgtgtg
     2101 gcctgccaca cgcgtcaatc ggccgtcctc gcgccgcgct tggacggcat cttcggccgg
     2161 ccggtgcggc tggcaaatgg tcagaccgtc attgccgacg aagactacat tcgcgagtcg
     2221 atcatgtacc ccaacgccaa gatttcagcg ggctatcccg cgccttccct gatgccgacc
     2281 tacgcgggcc aggtcagccc ggccgacctg cgcgacctgg tggaattcat caaggcactg
     2341 cgtgatggct ggccatcgga ggaccataca catggcgaac attgaaggcg ccccggccgt
     2401 aacagactac ttgcagaccg gctggcgggc gcgcgactgg tttctctcca cggaccataa
     2461 gcgggtggca attctgtact tgctgtcaat cacgtttttc ttctttatcg gtggattggc
     2521 agcaacgctg atccgggtcg agctgctcac gccgcccagc aacctggtca gcaaagacgt
     2581 gtttaaccgc ctgtttacaa tgcacggcgt aatcatggtc tggtttttcc tgatcccctc
     2641 gatcccgact accatcggca actttgtaat tccattgatg atcggcgctc gcgatctcgc
     2701 ctttcccaaa ctcaacctgt tcagttggta tctcttcagc gcgggcggac tattcacgct
     2761 gggcgcaata atcctgggcg gagtggacac cggttggaca ttctatacgc cgtacagttc
     2821 gttttacgct cattccgcag tggtgctggt catcctcggc atcgtggtcg ccgggttcgg
     2881 ctcgatcctg accggcctca acttcatcgt caccgtacac acaatgcggg cccctgggat
     2941 gggctggttc cagttgccgc tgttcatctg gtcccactat gcgaccagtc ttatcctcgt
     3001 gctcgccacg ccgatcctgg ccattactct tctgctggta ggcatcgaac gcgtctggca
     3061 atacggcatc ttcgagcctt cgctgggggg cgacccactg ctgttccaac atctgttctg
     3121 gttctattcg cacccggccg tgtacatcat ggttctgccg gccatgggcg tcgtgagcga
     3181 gattgtgccc tgcttcgccc gcaagcatat cttcggctac cacttcgtcg cgttcgccag
     3241 cctggcgatc gcgacctacg gcttcttggt ctggggccac cacatgttcg tctccggcca
     3301 gagcccgtat gcctcgctga ttttctccgc gctatctttc ctggtggcgg tgccctcggc
     3361 gatcaaggtc tttaactgga ccgctaccct gcacaaaggc tacattcatt tgcgcacgcc
     3421 gatgctgtat gccctgggct ttatcgggct gtttgtgatc ggcggcttga ccgggttgtt
     3481 cctggcatcg accgcgctcg acgtgcacgt acacgacacg tacttcgttg tttctcattt
     3541 tcattacatc atggtgggcg gggcggtaat gggatacttg ggcgcaatcc acttctggtg
     3601 gccaaagatg agcgggcggc ggtatccgga aggctggggc atttttgccg cgattctcct
     3661 gttcgtcggc tttaatctca ctttctttcc gcagttcctg ctgggctatg gcggaatggt
     3721 gcggcgcatc gcggagtacg cgccgggatt ccaggtcctg aatgtgatgt caaccgcggg
     3781 ggcttcgata ttgggcgtgg gttatgtgct gccgctggct tatctgctgt ggactctgcg
     3841 acggccgccg gacgccggac cgaacccgtg gtttgcgacc gggctcgagt ggcaaaccag
     3901 ttcgccgccg cccaagcaca acttcgtggc cgcgccgcag gttggcgaac cgtacgacta
     3961 cgccgaaatg ccgcgcgata tcccgtggag ctgaccaaag tggaaacgcc ggtcgaaatg
     4021 cagttcgacg atcccgccca gcaacgggag gcgtccgcgc tgggaatgtg ggcgttcctg
     4081 gccagtgaag tgctcttctt cggcggcgtg ttcaccggct actcaatcta tcgttggaag
     4141 tatccgcagg ccttcgccga ggccagccac cacttgtacg agtggatcgg agccgtaaat
     4201 acggccattc tgctgaccag cagcctgttc atcgcattgg ccgtgcaggc tctgcacact
     4261 gaacgcccgc ggcgcgcctg gcgcctgata attgcgacgc tcatctgcgg cttggcgttt
     4321 ctgagtctca aggcggttga atactggatg gactaccagg aaggacttgt gcccggtgtg
     4381 cggttcgacg cctcgaagtt tgcagaaccc ggcccggcga cgctgttctt caccttttat
     4441 ttcagcatga ccggcctgca cgcggcgcac gtaatctcgg gcctgctggt gctgaccgtc
     4501 atcggcgtaa cgctgcgacg cgccgccgat cccggcaaat acgccaattc gctggagaac
     4561 ggcgccttgt tctggcacct ggtcgacatc atctggatct tcctcttccc gttgctctac
     4621 cttatcggag ggccgaaatg accggcgggc cgcgcactgc aaccctctat tggacggccg
     4681 tgacgctggg cctgctgctg ggcggcaccg tcgtcgccac cgagttcctg ggccgggccg
     4741 gcccaaccgt gaatctggtc atcgcggcgg tgaaggccgc actcgtggcc gtggttttca
     4801 tgcacctgcg ctggagcagc ccgctgcagc ggctcttcgc gggggcggcg ttcttctggc
     4861 tggccctcct gtttgcgctg acgttcgcgg actacctgac gcgccgggcg tgagccgcat
     4921 tagccctgcg cttaacggtc gcacggcatg agcgcggccg gtgattctga ccgcaggcag
     4981 gcggggcttg caggccgcgc agcgccgcga gtgagaactc gaccacatgc tcgataagct
     5041 cgtcgggcgt cgcctccggc gcggcgggga acatgcgctg taccagatga ccgtgataaa
     5101 cgtggaagac agcgcgagcc accacaccgg ccgcagccag cgtgagttgc tggaaggaca
     5161 agtccggccg gatttccccc atggtttgtg acaagcggtg tgcagtgggg cgcaccaggt
     5221 cgtccatcaa atcaaggacc ggagtgggct gaaaaagttc acggcccagc acgcgcgttt
     5281 gccacttggg acggctcggg tcgagggcgg atttgagcat ggccgtaatc agttcgcgga
     5341 aacggccctc cggggtcttt gcggtaagcg gccggctcat ggccgccacg atgccctcgt
     5401 gcgcccggcg cagcacctgc gcgtagagct cgaacttatc atgaaagtga taattcacgg
     5461 atgcgacgtt ggcgccggcc cgctgcgtga tatcgcggat tgaggtaccg tgcaggccct
     5521 tttcggcgaa gatctcgccg gccgcttcga ttagcttttc gcgtgtcacg ggggccttaa
     5581 gccgacgcgg tcggtgcatg gcactcacct cgaaattggg cgcagtcgcc agcgctacac
     5641 ggccagaata atcccgccgc ccggcgctgt caaacgcgcg ttttaaacaa ccatttgaca
     5701 ggcggccggg gcatccctta acatgccccc caagtaattg cgcagcccaa acgggaatgg
     5761 aaccgcggat ttgggccagg cctggctcag aaggtacaag atgacaatcc gaaatcgacg
     5821 cccgctcggc gggcgcatgt cgcggctggt gcgcggcagc ttgttcctcg cggcgccggc
     5881 tgccttggcg gccttgatcg gctgcaacag cagcccgcag ccgcggccgc aaactgaggc
     5941 tccgccgccg gaggtggcgg tcgttacgct cgaggcacag ccggtgtcgc tgacgacgga
     6001 gttgcctggg cgaacgagcg cctaccgcat cgccgaagtg cggccgcgcg tgggcgggat
     6061 cattgaaaag cggttattcg cggaaggcac tgacgtcgaa gccggccagc agctttatca
     6121 gattgactcg cgccgcttcg tggcccagtt ggatcacgcg gatgcgaacg tcaagcgcgc
     6181 gcgggccgag ttagcccggg ccgattacga gctgcaacgt ctcaccaaac tccggcagag
     6241 cgatgccgcc gcggacaagg aataccagga cgccctcttc gcccagcaag cagccgaagc
     6301 cgcgttggac ctggctcttt ccgaacagcg gctggcggcg ttgaacctgg aatggacggt
     6361 ggtggtggcg ccgatttccg gacgcatcgg ctattcgtcg gtgactgagg gggcgctggt
     6421 cacggccgag cagcccacgc ctctgaccac gatccagcag atcgacgaaa tctacgtgga
     6481 cgtcacgcaa tcatcggccg acctgctgcg cctcagacgc caatgggaaa caggcgcgat
     6541 cagcagcggc gacggaatcg tgcggctgtt gctggaggac gggacgccgt atccgctgga
     6601 cggcaagctg cagttccgcg acatcacggt ggacccaacg accgggtcgt acacactgcg
     6661 catcgtgttc cccaatccgg accatctgct actgccggga atgttcgtgc gggcggtcgt
     6721 gcacgagggc acggccccgc aggcgctcct ggtgccccaa cagggcgtaa gccgcaatcc
     6781 caagggcgag ccggtggcgc tgctcgtgga tgaagccaat aaggttcagc agcggaaact
     6841 gacgctgaac cggcctatag gcaaccgctg gctgatttcg gcgggtttgt cgcccgggga
     6901 tcgcctgatc gtcgaaggcg cgctgcgtgt gcgcccaggt gacctggtga aggccgttcc
     6961 gtttgaggag ggcaaacaga aggtggccgt gggcgaggca aagaacgtta cgggcggctc
     7021 ggaatagcgg agcactccgg tgttatcgaa tttcttcctt gaccgcccgg ttttcgcctg
     7081 ggtcatcgcc atcatcatga tgctcgcggg cggcctggcg atttacaatt tgccggtctc
     7141 gcagtatccg ccgatcgcgc cgccctccat cgccatcgac gcgttctatc cgggggccgc
     7201 ggccgaaacc gtcgagaaca gcgtagtcca gatcatcgaa cagaagatga cgggcttcga
     7261 caagctgctg tacatgacgg ccatcagcga ggcctcgggc ggcgggcgga tcgagctcac
     7321 ctttgcgccg ggcactgatc cggatctggc gtgggcccag gttcagaaca agctccagct
     7381 cgcgatggcg agtctgcccg acgtggtgca gcgccagggc gtgaaggtca gtaaatccac
     7441 gcgcaactgg ctgctcatcg taggactggt ttccaacgat gacagcgtgg atgacattga
     7501 cctgcgcgac tatgcggcgg ccaatatcga gaaggtcctg gcgcgcgtgc ccggcgtggg
     7561 cgaagttgag gtgttcggat cgcagtatgc catgcgcatc tggctaaatc cggacaagct
     7621 gacaaattac aaactgacga tcgatgacgt gatcgccgcc ttgcgggctt acaacgtgga
     7681 gatttccgcg ggccagttcg gcgcggcgcc ggctgtcgaa gggcagcgct tgaacgcctc
     7741 gatcatcgtt cagaacctgc tcaagacacc ggaggagttc gctgaggttc ccctgcgcac
     7801 caatcctgac ggctccgtgg tgcgcgtgcg ggacgtgggg cgcacggaac tgggcaccga
     7861 gcgctacgag atcaacgcgc tgtacaacgg acgcccgtgc gccgcgctgg cggtacggca
     7921 ggccgccggg gcgaacgcgc tggagacggc ccgggccatc aaggccacaa tggacgagct
     7981 ctcccagtac ttcccgccgg gcatgggcgt catttacccg tatgacacca cgccgttcgt
     8041 cagagtcgcc atcaacgagg tggtgaagac cctgctcgag gcgatcgtgc tggtgttcct
     8101 ggtgatgtac ctgttcctcg gcaacattcg cgccaccctg gttccgacca tcgccgtgcc
     8161 ggtggtcatt ctgggcacct tcgccgtgct cggcgcgctg gggtactcga tcaacatgct
     8221 gaccatgttc gcgatggtgc tggccatcgg cttgctcgtg gatgatgcga tcgtggtcgt
     8281 ggagaacgtg gaacgcgtga tgcacgagga ggggctgtcg ccacgcgagg cgacgcgcaa
     8341 gtccatgcgc gaaatcacca gcgcgctggt cggcatcggc acggtgttgg cggccgtgtt
     8401 cgggcccatg gccttcttcg ggggctcgac gggcgtgatc taccgccagt tctcggcaac
     8461 tatcatcgca gccatgctgc tctcggtcgt cgtggccctc attctcagtc cggtgctgtg
     8521 cgcaggactg ctcaagcccg tggcccacgg gcacgcgccg gcggataccg gcttcgttct
     8581 gttccgcccc ttcttccgca tttttgaccg ggtctttttc tggatcagag acaggtaccg
     8641 ggacatggtg gggcgaatcc tggggcggaa gatccgctac ctgctggtgt acgtagtgct
     8701 ggtcgccgcg cttggatatc tgttccagcg tatgcccact tcgtacctgc cggaagaaga
     8761 tcagggcatg atgttcgtgc aagccatgct gcccgtcggt tcgacactgg agcaaaccga
     8821 ggcggtcatg aacgatgtgc gcaagtactt cgaggaacac gagaccgaag cggttgcctc
     8881 ctgcatgacg atttccggcc gcgctttctc cggcgggggt caaaacgtcg ggatggcctt
     8941 cattagactg caggactggg aactgcgtca gcggccggac ttgaaagtta aggccgtaat
     9001 cgggcgcgcg atgcaggctt tctcgcgaat ccgcaacgca caagtgtatg cgttcgcacc
     9061 gccggccgtg gtcgagctag gcacggccag cgggttcgat ttccaactgc aggaccgggg
     9121 cggactgggg cacgaggcct tgatggcggc gcgcaaccaa ttgctgagca tggccgctca
     9181 ggatccgcgg ctgatccgcg tgcgcccgaa cggtctcgag gatgtgccgc agtatcgcat
     9241 tgacgtggac tgggagaggg ccggtgtgct gggggtgccg attagtgcca tccacgacac
     9301 gcttgcggcc gccttcggca gcgcctacgt caacgatttc attcagggcg ggcgcgtcaa
     9361 gcgtgtttac gtgcaggccg acgccccgta tcgcatgctg cccagtgatc tggaacgcat
     9421 gtacgtgcgc aaccaggccg gattgatgac gcctttctcg gccttcgcat cggggcgctg
     9481 gagttacggc tcgccgcgtt tggagcgcta caatgcgttt ccatcgctca atatcttggg
     9541 tgaagcggcg ccggggcgga gttccggcga agcgatgcaa gcga
//
LOCUS       DAOSYS010000140         9567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6101_length_9567_cov_4.863856, whole genome shotgun sequence.
ACCESSION   DAOSYS010000140 DAOSYS010000000
VERSION     DAOSYS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_9567_cov_4.863856"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLU99_09715"
     CDS             complement(<1..238)
                     /locus_tag="PLU99_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17374.1"
                     /translation="MFGVHLKAGGVAMDAALAEVAAQADRILQRLWAPNELKQRLGDL
                     TAELKAAARIFCTGPVRVPEVGDLRFEPVIITPTR"
     gene            complement(335..3391)
                     /locus_tag="PLU99_09720"
     CDS             complement(335..3391)
                     /locus_tag="PLU99_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17375.1"
                     /translation="MLRPKLHVMIALVAVLLGGALGAPRPAAAQIEPEEPLLPVEISA
                     RDVEMRGRYVRQWKEDGTWILMFTGGFRLDMGQRHLSAHSGVVWITAAVDGEGRRYSV
                     FTVYLSENACVREFGGTTIEDNVLLVSNLRTYGKIIKYSDVHSPESGVQSPLYAQALR
                     DRAAIESGAVPPVAAPPAVVEVPAPGELEIARPEEAPEPEAPSGIVRYNVPGLEPALT
                     EEGLQVYVAIGQPQQRVYFSRAGGPTSPMLEILADNAVIFPAEDFAGGVLSGEEGEPE
                     IEAVEPAPGQGGPEGLRQAPENLPGEPAPEQGPLPTKAIGRNIRAVYLEGDVLLTLGT
                     SFIRACRLYYDFERDRAIILDATFRAELPDRNIPLYVRADEIRQLSARQFAATNAKVT
                     TSEFHTPHYHVGAEQIVLTDTTHRDAEGRAVGNTKGQYDIRDATFNVGGVPLLWWPRV
                     KGTFEESETSLRRIRAGYDDDFGAVLETEWYLFNLLGIPEPEGFDTTARLDYYSDRGP
                     GTGVNVDYERPTTYGLFRGYYIHDDGRDNLGPLRRAEEEPSTNERGRVLWRHRHYLPD
                     DWELTLEAAYFSDKNFLEEYETDEFFEGKQEETAFYLKRARGNEAVTLLSNWRMLESY
                     TATEHRPEVTYRRLGDTFLDPLVMYHESRFGMVTYRPAELTWDNLQFQLYNERFAPGP
                     IFDRNYRVTDSTARGDVRQEFELPLKLGAYNFVPFATLRGSYWDGQPRDSGALWRWFG
                     VGGVRASTIFSRVYDSIESALFDIHRIRHIIKPEVVAWSAGSNVRSDELTPFTYGIET
                     IDPFYGFGARVAQTWQTKRGPAGNERSVDVVTLNLGAGFFGNAEDRPDISDGWVDPMR
                     PENSWTNNYIAGDAPGRITGTTALLYDFNIDTNDWRLDRHNVSFVVERSPRLSYVLGY
                     REAKPLDLELIGGGWNYKLTEKYTSAFRGWYDLSRGEVGEVTVAFIRKLPRWYVSVAL
                     AYDEVEDDYSISFSIWPEGVPEWALGPRRFVPLGSSTGIRPGPAVR"
     gene            3615..5102
                     /gene="purF"
                     /locus_tag="PLU99_09725"
     CDS             3615..5102
                     /gene="purF"
                     /locus_tag="PLU99_09725"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOO17376.1"
                     /translation="MNREVRHYCGLFGIAGHPDAAHLTLLGMYALQHRGQESAGICVA
                     DGQQVTRIAGMGLVTDAIDQRELEKLRGPMAIGHVRYSTTGSCSESNVQPLIVRCSIG
                     QVAICHNGNLVNAAEIRRQHEEHGHIFITTSDTEVILHMLADRDFASQPDPLAAVLRH
                     LTGSYCLLLAYPDRIEAVRDPSGNRPLCLGRQGEAWVVASETCALDMIDAEYVRDVEP
                     GEIITLTPHGMQSRRFVDQSQIRRGYCIFEHVYFADPSSLVFGENVHLVRKEMGRALA
                     QEAPADADLVIPVPNCARCAAMGFHEQSGIPVGRGFTTNHYVGRSFIQPTQYIRDLTV
                     RMKLNPIRAVVAGKRLVVVEDSIVRGTTTRGKMASLRKAGAREIHLRVASPPIRHPCY
                     YGIDFPSREELVAHTRTVEEIRDYLEVDSLAYLSLEGMLAAAKTGNGDFCHACFSGEY
                     PIPIDPTFQKDIFERHQLRFELVANDKNGASLPVLQTGPPTRPRS"
     gene            5105..7042
                     /locus_tag="PLU99_09730"
     CDS             5105..7042
                     /locus_tag="PLU99_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17377.1"
                     /translation="MKTRSAVTLESVAPASCRPRSRVARYAPWLLIVIGAAVFANSLG
                     APFVFDDVAIPEGRHLRTLWPPWSAIFAPRDLPDSGRPVVTLSLQLNYALGGTDVRGY
                     HALNIAVHVLASLVLYGIVRRTLRGPALGDRFKNSADGIALVTALLWLVHPLQTECIA
                     YVSQRSESIMGLFYLLTLYCAIRVAHAARPWRWNSAAIVACALGMASKEVMVTAPLMV
                     VFYDWAFRREPFSAVWRRRWPLYAGLAATWIILAALMAAGPRTRSVGWHLGVGPLTYL
                     LNQCLIIPRYLRLIFWPHPLLLDYGQARSIPVATALPYAVGLAVVLIAVVAVCWRRPR
                     LGYAAAWVFVILAPTSSIVPIASEVGAERRMYLPLAGAIVLVVLAVHALLQRAETRKS
                     KASLSKYAGPALALAFTITLAATAIARNQAYYHPEELWAAAIEAYPQNKRAYFCHGVA
                     LEQRGRRDEAIQSYLKALALAPGYYEANYALANALIEQGRVAAAISYYRTALAAARRP
                     VDRAPVCNDLGRVLLMAGEPKEALQLWREAAGLKPDEAGLLNNLAWQLATHPDSTVRD
                     GREAVVLAERACELTGRQDPNMLDTLAAAYAEAGRFPEAVETARATCALLAGTEAAEA
                     RARLELYLQSKPYRAALPSEN"
     gene            7255..9255
                     /locus_tag="PLU99_09735"
     CDS             7255..9255
                     /locus_tag="PLU99_09735"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17378.1"
                     /translation="MAQHPINDARVYWEWAGDIAAGKLVLDHPFFSAPLYPYLLGGLR
                     WLGGQLTSVYVLQMLADLAAVVLLGWTCRRRFGAGVALGAVALYLLLLEPASAMLRVL
                     ASSLHLLLVVAAWAALVYVQERQSWPRLLAAGAALGLLTVAFAPAILLLPMVAIWLFV
                     LGDRKRESVVKALLPIAVAAVIVTPVTLHNWYAGGGLFPVQAGAGITLLQGNHATSNG
                     SYTPIPGVSTSRDRMHLDTKNVYQQATGRQPNWADVDRYFRNQAFELWRSQPAKLIRL
                     LALKSYMFVSARNYADIYEPTGEMAEGLNPRLRLAPLPLAWFIGPALVGLVLMLRKPG
                     HYAPEWMFLLVPFAVVVTFFYSPRYRTPAIPIIVVMAAWTFAQGWQWRRRWPVLAAAV
                     LALVVGMLQEPINKSFGFDRPDRTKALKNLAYALRMEHRVDDAIAAYRKCLAIDPQDS
                     ETRTWLGEALAEAGRREEGIIELRRALDEQPEDTTLAVKLANSLLLLNRPAEAEPVLA
                     AAALREPDNPLVLAMLANTKHRAGQNTAACEYFEQALQLAPDHVGILIDYAIALMNMR
                     RWDAAKVQFTRVLSVDPNSFLASFRLGVIAGHLGDNDEARRWFERASTIQPGNAEVVY
                     RLAYVELEQGRREEAIRLLRKALELDPNHRPAQAKLRELESR"
BASE COUNT         1654 a         3300 c         2878 g         1735 t
ORIGIN      
        1 accgtgtggg cgtaatgatt acgggctcaa atcgcaggtc gcctacttcg ggaacgcgca
       61 ccggccccgt acagaagatg cgggccgcgg ctttgagttc agccgtgagg tcgcccagcc
      121 gttgcttcag ctcgttggga gcccaaagcc gctgcagaat gcggtctgct tgtgccgcga
      181 cctccgccag cgccgcgtcc atggcgacgc cgcccgcctt caaatgaacg ccgaacacct
      241 gcggcgccgg ccgttcggcg ccgctgccga cgcttgtccc aaacgaagcc gagtcaagcc
      301 ctgacatgcc ccgagtatac gccgggtctg acgcttaccg caccgctggg cccggccgaa
      361 tgcccgtcga gctgcccagc ggcacaaaac gccgcggccc caaggcccac tccggcacgc
      421 cttccggcca gatggagaac gaaatgctgt agtcgtcctc gacttcgtcg taggccagcg
      481 ccacggatac gtaccagcgc ggcagcttac ggatgaacgc caccgtcacc tcgcccactt
      541 cgccgcgcga caggtcatac caaccgcgga acgctgacgt gtacttctcg gtcagcttgt
      601 agttccagcc gccgccgatc agctccaggt ccagcggctt cgcctcgcgg tagccgagca
      661 cgtacgaaag ccgcggcgaa cgctcaacca cgaacgagac gttgtgccgg tcgagccgcc
      721 agtcgttcgt gtctatgttg aagtcgtaca gcagggcggt cgtgccggtg atgcggcctg
      781 gcgcatcacc ggcgatgtag ttattggtcc agctattctc cgggcgcatc gggtccaccc
      841 agccatcaga gatatccggc cgatcttctg cgttgccgaa gaagcccgcc cccaggttaa
      901 gcgtgaccac atccaccgag cgttcgttcc ccgccgggcc acgcttggtc tgccaggtct
      961 gagccacgcg cgctccaaac ccgtagaacg ggtcgatcgt ctcgatccca tacgtgaacg
     1021 gcgtcagttc atcgctgcga acgttgctgc ccgcgctcca cgccacgacc tccggcttga
     1081 tgatgtgccg gatgcgatga atgtcgaaca gcgccgactc gatggaatca tacacgcggc
     1141 tgaagatcgt gctggcacgc acaccgccca ctccgaacca acgccacaag gcgcccgaat
     1201 cgcgcggctg cccgtcccaa tacgacccgc gcaacgtcgc gaacggcacg aagttgtacg
     1261 ccccaagctt cagcggcagc tcgaattcct gccgcacgtc gccgcgggcc gtcgaatccg
     1321 tcacccggta attccggtca aagatcgggc cgggcgcgaa gcgctcgttg tacaactgga
     1381 actgcaagtt atcccaggtc agttccgcgg gcctgtatgt caccatcccg aagcgcgact
     1441 cgtggtacat cacgagcgga tcgaggaacg tgtcgcccag gcgccggtag gtcacctccg
     1501 gccgatgctc ggtggccgtg tacgattcga gcatgcgcca gttggacagc agcgtgacgg
     1561 cctcgttccc gcgcgcccgc ttcaaataga aagccgtctc ttcctgcttg ccctcgaaga
     1621 actcgtcggt ttcgtactcc tcgaggaagt ttttgtcact gaagtaggcc gcttcgagcg
     1681 tcagctccca gtcgtcgggc agataatggc gatggcgcca cagaacccgc ccgcgctcgt
     1741 tggtgctcgg ctcttcctcc gccctgcgca gcgggccgag attgtcgcgc ccgtcatcgt
     1801 ggatgtaata gccgcggaac aggccgtaag tggtcggccg ctcgtagtcg acgttaaccc
     1861 ccgtgccggg cccgcggtcg gagtagtaat ccaggcgcgc ggtcgtgtcg aagccctccg
     1921 gctcgggaat gcccagcaga ttgaagaggt accattcggt ttcgagcacc gccccgaaat
     1981 cgtcatcata cccggcgcga atgcgccgca agcttgtctc gctctcctcg aaagtgccct
     2041 tgacgcgcgg ccaccacagc agcggcaccc cgcctacgtt gaacgtggcg tcgcgaatgt
     2101 cgtactggcc cttggtgttt ccgaccgcgc gtccctccgc gtcacggtgg gtcgtgtcgg
     2161 tgagcacgat ctgctcggcg cccacgtgat aatgcggcgt gtgaaattcg ctggtcgtga
     2221 ccttcgcatt cgtggcggcg aactggcggg ccgaaagctg ccgaatctcg tccgcccgca
     2281 cgtatagcgg gatgttgcgg tcgggcaact cggcacgaaa agtcgcgtcc agaataatcg
     2341 cgcgatcgcg ttcaaaatcg tagtacagcc ggcaggcgcg gatgaaactc gtgcccagcg
     2401 tgagcagcac gtcgccttcg agatacacgg cgcggatgtt ccgcccgatg gccttcgtcg
     2461 gtagcggtcc ctgctccggc gccggctccc caggcaggtt ctccggcgcc tggcgcagcc
     2521 cctctggtcc gccttgcccg ggcgccggtt cgacagcctc gatttccggc tcgccctctt
     2581 cgccgctcaa aacaccgccc gcgaaatcct cggccggaaa gatgaccgcg ttatcggcca
     2641 gaatttccag catgggtgac gtcggtccgc cggcccgcga gaagtacacc cgctgctgcg
     2701 gctggccgat cgccacgtag acctgcaatc cctcctcggt cagtgccggc tccaggccgg
     2761 gcacgttgta acgaacaatc cccgacggcg cctcgggctc tggcgcctct tctgggcggg
     2821 cgatctcgag ctcgcccgga gccggcactt cgaccaccgc cggcggcgcc gctaccggcg
     2881 gcactgctcc gctctcgatc gccgcccggt cgcgcagcgc ctgtgcgtaa aggggcgact
     2941 gtactccact ctctggcgaa tgtacgtcac tgtatttaat gatcttgccg tacgtccgca
     3001 ggttgctgac cagcagcacg ttgtcctcga tcgtcgtgcc gccgaattcc cggacgcagg
     3061 cattctcgga caggtaaacc gtgaacacgc tatagcgccg gccctccccg tcgacggccg
     3121 ccgtgatcca taccacgccc gagtgggccg acaggtgccg ctgccccata tcgagccgga
     3181 acccgcccgt gaacataaga atccaggtcc cgtcctcctt ccactgccgc acatagcgcc
     3241 cgcgcatctc aacgtcacgg gcgctgatct caaccggcag cagcggctcc tccggctcga
     3301 tctgcgcggc tgcggggcgc ggcgcgccca aggcgccgcc cagcagcacg gccaccagcg
     3361 ctatcatcac atggagcttc gggcgcagca tggctgcgcg ccagctccga caaggcgact
     3421 gagcttgatc cgtcacagac cgcacgacgg ctctcgaaag ggagtcgaaa tcctcgcttc
     3481 cgcgggtcat tatccagcag gagcgcggca ggtcaatggc gcggccgaga agcacgccac
     3541 tttcaaacgc ccggccggcg cattcgaggc ggacggctgt tgcatgccgg gccgccagcc
     3601 ggtataggta ttgaatgaac cgagaagtcc gccactattg cggtctgttc ggaattgccg
     3661 ggcacccgga cgccgcacac ctaacgctcc tcggcatgta cgccctccaa caccgcggtc
     3721 aggaatcggc cggcatctgc gtggccgacg ggcagcaggt aacccgcatc gccggcatgg
     3781 gcctggtcac cgacgccatc gaccagcgcg agctggagaa gttgcgcgga ccgatggcca
     3841 tcggtcacgt gcgctactcc acgacgggct cgtgcagcga gtccaacgtg cagcccctga
     3901 tcgtccgctg ctccatcggc caagtcgcca tctgccacaa cggcaacctg gtaaacgccg
     3961 cggagattcg gcgacagcac gaagagcacg gccacatatt catcacgacc tcggataccg
     4021 aggttattct gcacatgctg gccgaccgcg atttcgccag ccaacccgac ccgctggcgg
     4081 cggtgctgcg gcacctgacc ggctcgtact gcctgttgct ggcctatccc gaccgcatcg
     4141 aagcggtgcg tgatccctcc ggcaaccgcc cgctgtgcct gggccgccag ggcgaggcct
     4201 gggtggtcgc cagcgaaacc tgcgccttgg acatgatcga cgccgagtac gtccgcgacg
     4261 tcgagccggg cgaaatcatc accctgactc cgcacggcat gcaatcacgg cgtttcgtcg
     4321 atcaaagcca gatccgccgc ggctactgca tcttcgagca cgtgtacttc gccgacccct
     4381 ccagcctggt gttcggcgaa aacgtacact tggtgcgcaa agaaatgggc cgcgccctgg
     4441 cacaggaagc cccggccgac gcggacctgg tcatccccgt gccaaactgc gctcgctgcg
     4501 ccgccatggg cttccacgaa cagtcgggca ttccggtcgg gcgcggcttc accaccaacc
     4561 actacgtcgg ccgctcgttc attcagccca cgcagtacat ccgcgacctg acggtgcgca
     4621 tgaagctcaa cccgatccgc gccgtggtgg ccggaaagcg cctggtcgtg gtcgaagatt
     4681 cgatcgtgcg cggcacgacc acgcgcggca aaatggcctc gttgcgcaaa gccggcgcca
     4741 gggaaattca cctgcgcgtc gccagtccgc ccattcgcca tccctgctac tacggcatcg
     4801 actttcccag ccgcgaggaa ctggtcgccc acacccgtac ggttgaggaa atccgcgatt
     4861 atctcgaagt ggactcgctg gcctatctgt cgctcgaagg catgctggcg gccgccaaaa
     4921 ccggcaacgg cgacttttgc cacgcgtgct tttccggtga gtacccgata cccatcgatc
     4981 cgacgtttca gaaggacatc ttcgagcgcc atcagctccg cttcgaactg gtcgccaatg
     5041 acaagaacgg tgcgtcgctg ccggtcctgc aaaccggacc gcccacgcgc cctcgcagct
     5101 agccgtgaaa acgcgctcgg ccgtcacgtt ggaatccgtc gcgccggctt cctgccggcc
     5161 gcgctcgcgc gtagcgcgtt acgcgccgtg gctgctgatc gtcatcggcg cggcggtgtt
     5221 cgccaacagt ctcggcgcgc ccttcgtttt tgatgacgtg gccattcccg agggccggca
     5281 cctgcgcacg ctctggccgc cctggagcgc gatattcgca ccgcgcgacc tgcccgattc
     5341 cggccggccg gtcgtcacgc tctcgttgca actcaactac gcgcttggcg gcacggacgt
     5401 ccgcggttac cacgccctga acatcgccgt ccacgttctc gctagcctgg tgctctacgg
     5461 catcgtgcgg cgcacgctcc gcgggccggc cctgggcgac cgtttcaaga attccgcgga
     5521 cggcattgcc cttgttacag cgctactatg gctggttcac ccactgcaga ccgaatgcat
     5581 cgcctacgtc tcgcagcgca gtgaatccat catggggctg ttctacctgc ttacgctgta
     5641 ttgcgcgatt cgcgtggcgc acgctgcgcg cccctggcgt tggaactcgg cggccatcgt
     5701 cgcctgtgct ctgggcatgg ccagcaagga agtgatggtc acggccccgc tcatggtggt
     5761 gttctacgac tgggccttcc ggcgcgagcc gttctctgcc gtatggcgcc ggcgctggcc
     5821 attgtacgcc gggctcgccg ctacctggat catcctggcg gccctcatgg cggccggacc
     5881 gcgcacccga tcggtcggct ggcatttggg cgtcgggccg ctgacctacc tgctgaatca
     5941 gtgcctcatc atcccgcgct atctgaggct gatattctgg ccgcatccgc tcttattgga
     6001 ctacggccag gcccgaagca tacccgtggc gacggctctg ccttacgcag tcggtctggc
     6061 cgtggtgctt atcgccgtgg tggcagtctg ttggcgtcgc ccgcgcctgg ggtatgccgc
     6121 cgcgtgggta tttgtgatcc tcgcgccgac ttcgagcata gtcccaatcg catcggaagt
     6181 gggcgccgag cgccgcatgt atttaccact tgcgggcgct atcgttctcg tcgtacttgc
     6241 ggttcacgca ttgctacagc gcgccgaaac gcgaaagtcg aaggctagcc tttccaagta
     6301 cgccggccca gctctcgcgc tcgcgttcac catcaccctg gccgccacag ccatcgctag
     6361 gaaccaggcc tattaccatc ccgaggagct ctgggccgct gcaattgagg cttaccccca
     6421 gaacaaacgc gcctacttct gccacggcgt cgcgctcgag caacgcgggc gacgtgacga
     6481 agcgatccaa agctatctta aggccctcgc gctcgcgccc ggctattacg aggccaacta
     6541 cgccttggcg aacgccctca ttgagcaggg ccgcgtcgcc gccgcgataa gttattaccg
     6601 cacggccctg gcggccgccc ggcgtccggt cgatcgcgcc ccagtgtgca acgacctggg
     6661 ccgcgtgctg ctcatggccg gagaaccgaa agaagccctg cagctctggc gcgaggccgc
     6721 agggctcaaa cccgacgaag ccggcctgtt gaacaacctg gcctggcaac tggccacgca
     6781 ccccgattcg accgtccgcg acgggcgcga ggcggttgtc ctggcagagc gtgcctgcga
     6841 actcaccggc cgccaggacc cgaatatgtt ggataccttg gccgccgcat acgccgaagc
     6901 cgggcgtttc ccggaggctg ttgaaaccgc gcgtgccacc tgcgctctgt tagccggaac
     6961 cgaagctgcc gaggcgcggg cccgcctcga gctttacctg caatccaaac cctaccgggc
     7021 agcactgccg agtgaaaact gatgacaagc ggaaaacacg cgcattcaag ccgggcgcca
     7081 cgaaaggatt ctaaggaccg cgccgctgcg ccagtcgcaa cgaaacgtac gtctggtccg
     7141 agcaacgaac aaacctcctg gcccgaccga tatttcattc cggctctaat cggcctctgc
     7201 ctcaccgggc tggcgatccg ctgcctcatc ttggccggtt actgcggcac acctttggct
     7261 cagcatccaa tcaatgacgc ccgagtttac tgggaatggg ctggcgacat cgccgccggc
     7321 aagctggttc tggaccatcc gttcttttcc gcaccgctgt acccgtatct tttgggcggc
     7381 ctgcgctggc tggggggcca gctaacctcc gtatacgtgc tgcagatgct ggccgatctg
     7441 gctgccgtgg ttctgctggg gtggacttgc cgccggcgtt tcggggctgg agtcgccctc
     7501 ggcgccgtcg cgctctacct gctgctgctg gagccggcct cggccatgtt gcgcgtgctg
     7561 gccagttcgc tgcacctgct actggtagtg gcggcgtggg cggctttggt ttacgtccag
     7621 gagcgtcaat cgtggccgcg cctgctcgcc gcaggcgccg cattggggct gctcactgta
     7681 gcattcgcac cggcgattct actgctgccg atggtcgcca tctggctgtt tgtgctgggc
     7741 gatcgtaagc gcgagtcagt cgttaaggca ctgctgccga tcgcggtggc ggccgtgatt
     7801 gtcacccccg tcacactgca caactggtac gccggcggtg gcctattccc cgtccaggcc
     7861 ggggccggca ttacgctgtt gcaagggaat cacgccactt ccaatggctc gtatacgcct
     7921 attccgggcg tgagcacctc ccgcgaccgc atgcacctgg atacaaagaa cgtctatcaa
     7981 caagctacgg gccgccagcc gaactgggcg gacgtcgatc gctatttccg caaccaggct
     8041 ttcgaactgt ggcgttcaca gcctgccaaa ctgatcagac tgctggcgct caagtcttac
     8101 atgttcgtat ccgcccggaa ctacgccgac atctacgagc cgaccggcga gatggccgaa
     8161 ggcctcaatc cgcggcttcg cctggccccg ctgccgctgg cctggttcat cggcccggcg
     8221 ctggtcggcc tggtgctgat gctccgcaag cccgggcatt acgcgccgga gtggatgttc
     8281 ctgctcgttc cattcgccgt cgtggtgacg ttcttctact caccgcgtta tcgcacgccg
     8341 gctattccga tcatcgtggt tatggcggcc tggacattcg ctcaagggtg gcagtggcgg
     8401 cgacgttggc cggtactggc cgcagccgtc ctggcgctgg tggtgggcat gttgcaggaa
     8461 ccgatcaata agagcttcgg cttcgatcgc cccgatcgta cgaaggccct gaagaacctg
     8521 gcctacgcgc tgcgcatgga gcaccgcgtg gacgacgcta tcgccgcgta ccgcaaatgc
     8581 ctggccatcg atccacagga cagcgagacg cgcacctggc tgggcgaggc gctggccgag
     8641 gccggccgcc gcgaagaagg catcatcgag ctgcggcgcg cgttggacga gcagcccgaa
     8701 gataccactc tggccgtcaa gctggccaac agccttcttc tgctgaaccg gccggcggaa
     8761 gccgaaccag ttctggccgc cgccgcgctg cgcgaaccgg acaacccgct ggttttggcc
     8821 atgctcgcca acaccaagca tcgcgccggc cagaatacgg ccgcctgtga gtacttcgag
     8881 caagcgctgc aattagcccc cgaccacgtt ggcattttga tagactatgc cattgcgctg
     8941 atgaacatgc gccgctggga cgccgccaag gtccagttca caagagtact gagcgtggac
     9001 ccgaacagct ttttggccag ctttcgcctg ggcgtgatcg ccggtcactt aggcgacaac
     9061 gacgaagcgc gacgctggtt cgaacgcgct tcaaccatac agccgggaaa cgcggaagtc
     9121 gtctatcgcc tggcatatgt ggagctggaa cagggccgcc gcgaagaggc gattcgtctg
     9181 ctccgcaaag cccttgagct ggaccccaat catcgaccgg cgcaggccaa gctacgcgag
     9241 cttgaatcgc gctgaatgct gccgggccca cccgccgaga taaggtgata aagtgatagg
     9301 gtgataaaga accatccgcg gaaccccctt atcactctat cactttatca cttcatcact
     9361 ctggcctccg cgcagaatcc ccgtctcagt gctatgtccc gagaatggcg accgactcag
     9421 cactcaactc gatactctcg ccggacagcg cgtattgatt acctgacgcg agcagtgggc
     9481 gccacgactc accgcccgag ccggtttcgc cgaaatccag gcgcacttgc tgttgccgcg
     9541 acgaaaagtt gcatattacg gctgtcg
//
LOCUS       DAOSYS010000141         9517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6147_length_9517_cov_4.782498, whole genome shotgun sequence.
ACCESSION   DAOSYS010000141 DAOSYS010000000
VERSION     DAOSYS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_9517_cov_4.782498"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="PLU99_09740"
     CDS             complement(<1..1840)
                     /locus_tag="PLU99_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO17379.1"
                     /translation="MNAHLLKAVFPVVLLQLAAAPPPESCGWGAAPNAALTLSVVDAE
                     TRLALGCAAPFHRTPNQVANARLVPVENSPILIACWEETAASGAPQSYYAISLDGQTM
                     ATVRATSYALKLRQQEFDPVVRAPALPVELVADASGHIYIVQFVAQPLPEFRTAIEAR
                     GGTIYKFLPNHAYVVKMTPEVRDSVAALPFVRWVGPYHPAYRMEASLREQSRSAGLPA
                     ERFNIQVFEAGDDQKNAVAQRIEALGGVVDVRNAGKYLLRATLTPSQAVTVAHWDEVA
                     FIDHWAPVELAMDKFRLVGGVDYVEQVAGFDGAGVRGEVLDVGFNYQHVDFASRPLIQ
                     HTSSPGHFHGACCSGIIFGDGTGDPAARGICPAGQGITADWYVYESGSRYDYTAELLH
                     EPYYAVFQSSSCGTGRTLEYTTISAEIDTIIFDLDIVHLQAQANAGSALSMAEAWAKN
                     IISCGGVQHYDTLDLADDEWDHYASTGPALDGRIKPDMCFWVDFIHTVDYPGVRAYTD
                     EFGGTSGATPAIAGHVGLFFQMWSQGVFGNEFDPNGSVFDNRCHSSTAKAFLINTGNQ
                     YPFQGQSHDLTRVHQGWGRPSVQNMWDLRDQVYFVDETVVLRPFERY"
     gene            complement(1983..5222)
                     /locus_tag="PLU99_09745"
     CDS             complement(1983..5222)
                     /locus_tag="PLU99_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17380.1"
                     /translation="MKRLLPIMPLALAFSTIALGQLFPVTPAAGTTLPQAGLSVARDG
                     GRTHMALNGVSFHETPDVISAARLIEVPQSTVCLVCWEQTAPDGQLTPYYAISLDGEN
                     MATVRPTSYELKLQHSQFDPLGRIPAVETDLAADEDSQIYIVQFVTQPLEQFRTAIEA
                     RGGTLYKYIANHAYLVKLGPQARQAVAALPFVRWLGPYHPAYRLEHFLRANRGDAGQL
                     FPMQRYNILLFEREGAQDVVADRIAALGGEVNVRNAGRFLMQATLTPQQLFDVIRWDE
                     VQFIDRWSPAEPDIDLIRADGGADYIETVAGYDGAGVRGEVIDVGFCPGHVDFASRPL
                     IPHTSASIDTHGCSCSGIIFGDGTGDPTARGLCPAGQGIIADWGLIGSRYAHTAELLE
                     DPYYAVFVTASVGGFLTTSYTTVSADMDDMLFDLDILHCNSQSNAGSRDSRPEAWAKN
                     IVSCGGIYHYGTLDPADDCWCGGASIGPAADGRVKPDLVFYYDRIHTTSQAPAGYTDG
                     FCCTSGATPTVAGHFGLFFQMWADGIFGNPVIPGATVFENRPHMTTAKAALINTANQY
                     EFSGPSHDLGRMHQGWGRPSVANLYDLRNKIFVIDETELLQNLESYVCQAYVAPEEPA
                     LKVTMTYADPAGVPGAAQARINDLTLRVISPSGVEYWGNNGLLAGNWSTPGGAPDTIN
                     TVENVFVRNPEPGLWTVTVLANEINEDGHVETPELDADFALVISGTPGFALRLRLLDR
                     PPAYLTPGEPTPVVVAIDEGSETYVPGSGALHYSYDGGAFQQTELLPLGDNLYQGILP
                     AATCSATPRFYFSAAASGGGIVYSPPMAPEVPYSATVGVVVTAIADNFEQDLGWTVWN
                     DPSLTGGMWQRGIPVSTDVGQPPTADYDGSGKCWLTQNVAGGSDVDGGPTCLISPLLD
                     LTGQTDPVLQFARWWANDDQDGDPFDVEASSDGGTNWVLLQRIQNVQPEWVVETIHLA
                     DFIPQTDQVRLRFSVRDVPNNSVDEGGLDAVSIRGFNCTDGIDHGDLNCDGVVNVFDI
                     DPFVLALTDAAAYGAAYPGCSILNADCNGDGSINVFDIDSFVQCLTSGC"
     gene            complement(5356..7128)
                     /gene="hutU"
                     /locus_tag="PLU99_09750"
     CDS             complement(5356..7128)
                     /gene="hutU"
                     /locus_tag="PLU99_09750"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOO17381.1"
                     /translation="MPPTARTVRAPRGTTLSCKGWQQEAALRMLMNNLDPDVAEKPEE
                     LIVYGGRGKAARSWEDFERIVATLKRLENDETMLVQSGRAVGVLKTHPDAPRVLISNS
                     MLVPKWATWDEFRRLEALGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAARKHFGGS
                     LRGRLVVTGGLGGMGGAQPLAATFNDGVMLAAEVDPHRVERRLQTRYLDKSTHRLDEA
                     IAWAVEAKEANQAVSIAWVGNQADLLAELIRRNITPDVLTDQTSAHDPLNGYVPNGIL
                     GGTHASATTRPQSHGGEPWSEARYAALLRLRQEDPETYVRESFRTMNEHVRHMLELQK
                     RGAVTFDYGNNLRGHAVEAHERYRGAAGDARPFGIEPVDAFRIPGFVPEYIRPLFCEG
                     SGPFRWAVLSGDPRDLAATDEAVLATFPEEAHLCRWIRLAREKVAFQGLPARICWLKY
                     GQRARMGAVFNDLVRTGKVSAPIVIGRDHLDCGSVASPNRETEGMKDGSDAIADWPML
                     NALVNASSGATWVSIHHGGGVGIGYSLHAGMVIVADGTPEAERRLARVLTNDPATGVI
                     RHVDAGYDIAVRVAEQRGIDIPRC"
     gene            complement(7337..8365)
                     /locus_tag="PLU99_09755"
     CDS             complement(7337..8365)
                     /locus_tag="PLU99_09755"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOO17382.1"
                     /translation="MTLPLRPPVMPMEAEVAKELPEGAQWQYEPKWDGFRCLAFRDGD
                     DVNLQSKAAKPLSRYFPELVAALAALRPRQFVLDGEIVVPIEGRLSFDDLLQRIHPAE
                     SRIRKLSRERPALLIAFDVLVDEHGRLVADEPLSRRRAKLEAFARRVLPKSGSVRLSP
                     ATLDVDQAREWIHDAGWNLDGVIAKNRKLPYQHERTAGMLKIKRHRSADCVVGGFRYA
                     TSGRLVGSLLLGLYDDEGKLNHVGFTSGIKVSERPALTRKLEQLRKPPGFTGSAPGGP
                     SRWATERSTEWEPLAPRLVVEVQYDHFTGGRFRHGTRLLRWRPDKDPRQCKLSQVERE
                     SRAPLLVT"
     gene            complement(8362..9360)
                     /gene="ligD"
                     /locus_tag="PLU99_09760"
     CDS             complement(8362..9360)
                     /gene="ligD"
                     /locus_tag="PLU99_09760"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOO17383.1"
                     /translation="MPRARFASRGVKSKTATARAERTRITPRVEIPDENAAVLEVAGR
                     RVSLTNLRKVFWPELGLTKRDLLQYYADVAPVLLPHVRQRAMVMKRYPNGVAGNFFFM
                     KRAPAPRPDWIQLCAIEHDSGNVIHFPVVQDLPSLLWLVNLGCIDLNQWYAPCDDVNR
                     PDYLHFDLDPVPNATFEQVLETALHVHAALDRLKIPDYAKTTGSRGIHIYVPIVRGPV
                     QKQVWTFAKAFAQALAAEHPQLITAEYRIHKRPRNRVLVDYNQNAWGRTLASVYSVRP
                     TPQATVSTPVTWQEIERGIRITDFRLDNVPARVRRIGDLWKPLLQRGGRFRLERLL"
BASE COUNT         1721 a         3077 c         2957 g         1762 t
ORIGIN      
        1 tgtacctctc gaacggcctc agcaccactg tttcgtccac gaagtacact tggtcgcgca
       61 agtcccacat gttctggacg ctgggccggc cccaaccctg gtgcacccgc gtcaggtcat
      121 gcgactggcc ctgaaatggg tactggttgc cggtattgat caaaaacgcc ttggcagtcg
      181 acgagtggca acggttatcg aagacgctgc cattcggatc gaactcgttg ccgaagacgc
      241 cctgggacca catctggaaa aagagcccca cgtgaccggc gatggccggc gtcgcgccgc
      301 tcgtgccccc gaactcgtcg gtatacgctc tgacgcccgg gtaatcgacg gtgtgaatga
      361 aatccaccca gaaacacatg tcaggtttga tgcggccgtc tagggccggc ccggtgctcg
      421 catagtggtc ccattcgtcg tcagccaggt ccaacgtgtc gtagtgctgt acgccgccgc
      481 aggagatgat gttcttggcc caggcctccg ccatggacag agcactgccg gcattggctt
      541 gcgcctgaag gtgcacgata tccagatcga agattattgt gtcgatctcg gcggaaatgg
      601 tggtgtactc taatgtccgg ccggtgccgc aactggagct ttgaaagacg gcgtagtacg
      661 gttcgtgaag cagctcggcc gtgtagtcgt agcgcgagcc ggactcatat acataccagt
      721 cggcggtaat gccctggccg gccgggcata tgccgcgcgc agccgggtcg ccggtgccgt
      781 cgccaaagat tatccccgag cagcaggcgc cgtggaaatg gcccggcgag gaggtgtgct
      841 ggattagcgg gcgtgaggcg aaatccacat gttggtaatt gaacccgaca tccaggactt
      901 cgccgcgaac gcccgccccg tcgaaaccgg ccacctgctc gacatagtcg accccgccga
      961 ccaaccggaa cttgtccata gccagttcca cgggggccca gtgatcgatg aaggcgacct
     1021 cgtcccaatg cgcgacggtt acggcctggc tgggggtaag cgtcgcgcgc agcaggtact
     1081 tgccggcgtt gcgcacgtcg acgacgccgc ccagagcctc gatgcgctgg gcaaccgcgt
     1141 tcttctgatc atcgcctgct tcgaatacct gaatgttgaa acgctccgcc ggcaagccgg
     1201 cacttctcga ctgctcgcgc agggatgcct ccatgcgata ggcggggtga tagggtccaa
     1261 cccagcgcac gaatggcagt gcggcgactg agtcgcgcac ctccggcgtc atcttgacca
     1321 cgtacgcgtg gttgggaagg aatttgtata ttgtgccacc gcgggcttcg atcgccgtcc
     1381 ggaactccgg cagcggttgg gccacgaatt gcacgatgta gatatggccg gacgcatccg
     1441 caaccagctc aaccggcaac gcgggggccc gtaccacggg gtcaaactcc tgctgtcgca
     1501 acttgagcgc ataagaggtc gcgcgcacgg tcgccattgt ctgaccgtcc aaactgatcg
     1561 cgtaatagct ctgcggcgcg ccgcttgcgg ctgtttcttc ccagcacgca atcaggatcg
     1621 gtgagttctc gaccggaaca aggcgggcat tcgcgacctg atttggggtg cggtggaacg
     1681 gcgccgcaca acccagggca agtcgcgtct cggcgtccac cactgacagc gtcaaagcag
     1741 cgttgggcgc cgcgccccag ccacatgact ccggcggcgg cgctgccgcc aactgtagca
     1801 gaacgacagg aaacacagct ttcaaaaggt gggcgttcat gtcaagtctc cgaaccgata
     1861 ttgggcgcct taaccaagcc ggctgtggac cggctcaaga acgcgatggt ttacattctg
     1921 tggtccagcc ggcgtgagga ggcccgccgg ctggacctgt taggctgcca gtgccttcac
     1981 tcctagcagc cggatgtcaa acattgcacg aacgagtcga tgtcaaacac attgatcgac
     2041 ccatcaccat tgcagtccgc attcagtatg gagcagcccg ggtaggccgc gccataggcg
     2101 gcggcgtcgg ttaatgccag cacgaacgga tcaatatcga agacattgac cacaccgtcg
     2161 caattcagat cgccgtgatc gatcccgtca gtacagttaa agccgcgaat agagacggcg
     2221 tccaagccgc cttcgtcgac cgaattgttc ggtacgtcgc gcacgctgaa gcgcagtcgg
     2281 acctggtccg tctgcggaat aaagtctgcc aagtgtattg tttcgactac ccactccggc
     2341 tggacatttt gaatgcgttg caggagcacc cagttcgtgc cgccatcgct ggacgcctcg
     2401 acgtcgaacg gatcgccgtc ctggtcatcg ttggcccacc agcgcgcgaa ttgcagcacc
     2461 gggtcggttt ggccggtaag gtcgagcagc ggcgagatga ggcacgtcgg cccgccatcc
     2521 acgtcggagc cgcccgcgac gttctgagtg agccagcact tgcccgagcc atcgtaatcg
     2581 gcggtcggcg gctggcccac atccgtggaa accggaatgc cgcgctgcca catgccgccg
     2641 gtcaggctcg gatcattcca caccgtccaa cccaggtcct gctcgaagtt gtcggcaatt
     2701 gcggtgacca ccacacccac ggtcgccgag tacggaacct cgggcgccat cggcgggctg
     2761 tagactattc ccccgccgct agcggcagcg ctgaagtaga agcgcggcgt ggcgctgcac
     2821 gtggccgcgg gcagaatgcc ctggtacagg ttgtcaccca gcggcaacag ctcagtttgc
     2881 tggaacgcgc cgccatcgta gctgtaatgc agagcgcccg acccgggcac atacgtctca
     2941 ctgccctcgt caattgccac cacaaccggc gtcggctcgc ccggggttaa ataggccggc
     3001 gggcggtcca gtagtcgcag acgcagtgcg aacccgggcg tgccgctgat caccagtgcg
     3061 aagtccgcgt ccagctccgg cgtttccacg tgcccgtctt cgttgatctc gttggccaag
     3121 acggtgaccg tccacaagcc cggctcgggg tttcgcacga agacgttttc gacggtgttg
     3181 attgtgtcgg gcgcgccgcc cggggtggac cagttgcccg ccaacaggcc gttgttgccc
     3241 caatactcga cgcctgatgg agagatgacc ctgagcgtca ggtcattgat acgcgcttgg
     3301 gccgcgcccg gcacaccggc cgggtcagcg taagtcatgg tgaccttcag ggccggctct
     3361 tcgggggcca catacgcctg gcagacgtag ctttccagat tttgcaacag ctcggtttcg
     3421 tcgatgacga atatcttgtt gcgcaggtcg tacaggttcg ccacgctcgg ccggccccag
     3481 ccctggtgca tgcggcccag gtcgtgcgac gggccactga attcgtattg attcgcggtg
     3541 ttgattaagg ccgctttggc tgttgtcatg tgcgggcgat tctcgaacac ggtcgcgcca
     3601 ggtatgaccg ggttgccgaa gatgccgtcg gcccacatct ggaaaaacag cccgaagtgc
     3661 cccgcgacgg tgggcgtagc gccgctagta cagcagaagc cgtctgtata tccagccgga
     3721 gcctggctgg tcgtgtgaat gcggtcgtaa taaaacacca ggtccggctt tacgcgccca
     3781 tcggccgccg gtccgatgct ggcgcctcca caccagcagt cgtccgcggg atcgagggtt
     3841 ccgtagtggt atatgcctcc gcacgagacg atattcttgg cccaggcctc gggacgcgaa
     3901 tcccgactgc cggcattgct ctgcgagttg caatgcagaa tgtccaggtc gaacagcata
     3961 tcgtccatgt cggccgaaac ggtcgtgtaa ctcgtcgtca agaagccgcc cacgctggcc
     4021 gtcacgaaca cggcgtagta gggatcttcc agcagttcgg ccgtgtgagc gtagcgcgag
     4081 ccgatcagcc cccagtcggc gatgataccc tgaccggccg ggcacaagcc gcgcgccgtc
     4141 ggatcacccg tcccgtcgcc gaagatgatt cccgaacacg aacagccgtg cgtgtcgatg
     4201 ctagcggacg tgtgcgggat caacgggcgc gaggcgaagt cgacgtggcc ggggcagaag
     4261 ccgacgtcga ttacttcgcc gcgcacgccg gccccgtcat agccggcgac ggtttcgatg
     4321 tagtccgcgc cgccgtcggc gcgaatcagg tcgatgtccg gctcggccgg gctccagcgg
     4381 tcgatgaact gcacttcgtc ccagcggatc acgtcgaaca actgctgcgg cgtaagcgtg
     4441 gcctgcatca ggaaccgccc cgcattgcgc acgttgacct cgccgcccag cgctgcaatc
     4501 cggtcggcca ccacgtcctg cgcgccctcg cgctcgaaaa gtagaatgtt gtagcgctgc
     4561 attggaaaca actggccggc atcgccacgg ttggcgcgaa gaaagtgctc cagccgataa
     4621 gcgggatggt acggcccgag ccagcgcaca aacggtagcg ctgcgaccgc ctggcgggcc
     4681 tgcggcccga gtttcacgag ataggcgtga ttggcaatgt acttgtacag tgtgccgccc
     4741 cgtgcctcga tggccgttct gaattgctcc agaggctgag tcacgaattg cacaatgtaa
     4801 atctgcgagt cttcatccgc ggccagatca gtctcaactg cggggatccg ccccagcggg
     4861 tcgaactggc tgtgttgcag tttcaattca tatgaagtcg gccgcacagt ggccatattt
     4921 tcgccgtcga ggctgatagc gtagtacggc gtgagttgtc cgtccggtgc ggtctgttcc
     4981 cagcagacca gacacacggt cgattgcgga acttcgatca ggcgcgccgc ggagattacg
     5041 tccggcgtct cgtggaacga aacgccgttt agcgccatat gagtccgccc accgtcgcgg
     5101 gctacactta gcccggcctg tggcagcgtt gtgccggcgg cgggtgtaac cgggaacaat
     5161 tgtcccagtg caatcgtgga gaaggcgagc gctaacggca tgatgggcag cagacgcttc
     5221 atttcgactc tcctgagcaa ggaactgttc cagccgctgc gccctgaata tactgattgc
     5281 gccccaacca tgctaggtgg gtgtcggatt tccacttatt gccgcgttta taaggcgtgc
     5341 cgttacgtca ggtaactaac aacgcgggat atcgatgcct ctttgctccg ctacgcggac
     5401 ggcaatgtcg taacctgcgt ccacgtgccg aatcacgccg gtggccggat cgttagtcaa
     5461 cacacgggcg aggcggcgct ccgcttctgg tgtaccgtcc gcaacgatca ccattccggc
     5521 gtgcagcgaa tagccgatac ccacgccgcc gccgtggtga atgctgaccc acgtggcccc
     5581 gctcgacgca ttgaccagag cattcagcat cggccagtcg gcaattgcgt ccgagccgtc
     5641 cttcatgccc tcagtctcgc ggttggggct ggccaccgag ccgcagtcga gatggtcgcg
     5701 gccgatgacg atcggcgcgc taaccttgcc tgtgcgaacc aaatcgttga acacagcgcc
     5761 catccgtgcc cgctggccgt acttcaacca gcagatgcgc gccggcagac cctggaaggc
     5821 gaccttctca cgcgccaggc gaatccagcg gcagagatgc gcctcttcgg ggaaagtcgc
     5881 cagcacggcc tcgtccgtgg cggccaggtc acgcggatcg cccgacagca ccgcccagcg
     5941 gaacggaccg gaaccctcgc agaagagcgg gcggatgtat tcagggacga agcccggaat
     6001 gcgaaacgcg tcgaccggct caatcccgaa cggccgcgca tcgccggccg cgccgcggta
     6061 acgctcgtgt gcttcgaccg cgtggccgcg gagattgttg ccatagtcaa acgtgactgc
     6121 tccgcgcttt tgaagttcga gcatgtgccg cacgtgttcg ttcatggtgc ggaacgattc
     6181 gcgcacatac gtctccgggt cttcctgccg caggcgcagc aacgcggcat aacgcgcctc
     6241 gctccacggc tcgccgccgt gactctgcgg ccgggtcgtt gcggaggcgt gcgtcccgcc
     6301 gagaatgccg ttgggtacgt agccgttcag cggatcatgg gctgacgtct ggtcggtcag
     6361 gacgtcgggc gtgatgttgc ggcgaatgag ttcggccaac aggtcagcct ggttgccgac
     6421 ccacgcgatc gagaccgcct ggttggcctc tttggcctcg accgcccagg cgatggcctc
     6481 atccagcctg tgggtgctct tgtcgagata gcgcgtctgc aagcggcgct cgacgcggtg
     6541 gggatcgact tcagccgcca gcattacgcc gtcattgaat gtcgcggcca gcggctgcgc
     6601 tccgcccatg ccgcccagcc cgcccgtgac caccagccgg cccctcaacg agccgccaaa
     6661 gtgcttgcgg gccgccgcgg cgaacgtctc gtacgtgccc tgcaaaatgc cctgcgtgcc
     6721 gatgtaaatc cagcttccgg ccgtcatctg gccgtacatg gtcagcccga gagcctcaag
     6781 ccgccggaac tcgtcccagg tggcccattt cggcacgagc atgctgttgc tgatgagcac
     6841 ccgcggcgcg tccgggtgcg tcttgagtac gccgaccgcc cggccggact gcaccagcat
     6901 cgtttcgtcg ttctcgaggc gtttgagcgt agcgacgatc cgctcgaaat cctcccacga
     6961 gcgggcggcc ttccctcggc ccccgtaaac gatgagttct tcgggcttct cggccacatc
     7021 cgggtcgagg ttgttcatga gcatgcgcaa ggcggcttct tgctgccagc ctttgcagga
     7081 caacgtggta ccgcgcgggg ctcggacggt gcgtgctgtt ggcggcatgg tctgactcca
     7141 gactgcgttc ttctctggtt cggttaactc acggtcactt tatgccggac ttcgtagttt
     7201 atgcgtacct tacccgttgc caatatgagt tggccaccgc cccggattgc cggggcggac
     7261 catcgagagg gatcgaggga tcaagaggtg caaaaagcag actcgctcgc cccgcacccg
     7321 cgccgggcgc gaaatgtcac gtaaccagca gcggcgcccg gctctcgcgc tccacttgcg
     7381 acagcttgca ctgccgcggg tccttgtctg gacgccagcg tagcaggcgc gtgccgtgcc
     7441 ggaagcgccc gccggtgaaa tgatcgtatt gaacctcgac gaccagccgc ggagcgagtg
     7501 gttcccattc ggtcgagcgt tccgtcgccc atcggctcgg accgccgggc gcgctgcccg
     7561 taaaacccgg cggctttctg agctgctcga gctttcgagt cagcgctggc cgctcactga
     7621 ccttaatgcc cgaggtgaac cccacatgat tcagcttgcc ttcgtcatca tacaggccca
     7681 gcagcagcga cccgaccaac cggccgctgg tcgcataccg aaacccgccc accacgcagt
     7741 cggcactgcg gtgacgcttg atcttcagca tgcctgcggt gcgttcgtgt tggtacggta
     7801 gcttgcggtt cttggcgatg accccatcaa ggttccagcc ggcatcgtgg atccactcac
     7861 gcgcctgatc gacgtccaaa gtcgccggag agagccgcac cgacccgctc tttggcaaga
     7921 cacgccgcgc gaacgcttcc agcttcgccc ggcgccgcga aagcggctcg tcggccacaa
     7981 ggcggccgtg ctcgtccacg agtacgtcga atgcgatcag cagcgccggc cgctcgcgcg
     8041 acaacttacg aatccggctt tcggccggat gtatgcgttg tagcaagtcg tcgaacgaca
     8101 gccgcccttc gatcggcacg acgatttcgc catccagcac gaactgccgc ggtcgcagcg
     8161 ccgccaacgc cgccaccaac tccggaaaat accggctgag cggcttggcg gccttggatt
     8221 gcaggttcac gtcgtcgccg tcacggaagg ccaggcagcg aaacccatcc cacttcggct
     8281 cgtactgcca ctgcgcacct tccggcaatt ctttggccac ttcggcctcc atcggcatca
     8341 ccggcggacg aagcggcagc gtcacaacag ccgctccaag cgaaagcgcc cgccgcgctg
     8401 cagcaacggc ttccacaggt caccgatccg ccgcacgcgc gccggcacat tatctaagcg
     8461 gaaatcggtg atccggatgc cgcgctcgat ctcctgccaa gtcacgggcg tcgagacggt
     8521 cgcctgcggc gtcggccgga ccgagtagac cgaggccagc gtgcgtcccc aggcattctg
     8581 gttgtagtcg accagcacgc gattgcgcgg ccgcttgtga atgcggtatt cggccgtgat
     8641 gagttgcggg tgttcggccg ccagagcctg ggcaaaggct ttggcgaaag tccacacctg
     8701 cttctgcacc ggcccgcgaa cgatcggcac atagatgtgt atgccgcgcg agcctgtggt
     8761 tttcgcatag tccggtatct tcagccgatc gagcgccgcg tgcacgtgaa gcgccgtttc
     8821 gagcacttgt tcaaaagttg cattcggtac cgggtcgaga tcgaagtgca ggtaatccgg
     8881 ccggttcacg tcgtcgcacg gggcgtacca ctggttcagg tcgatgcagc cgaggttgac
     8941 gagccacaac aggctgggca agtcctgcac gaccgggaag tgtatgacgt tccccgagtc
     9001 gtgctcgatg gcgcaaagct ggatccaatc ggggcgcggt gccggggccc gtttcatgaa
     9061 gaaaaaattc ccggccacgc cgttcggata gcgcttcatc accatggcgc gctggcgtac
     9121 gtgcggcagc agaaccggcg cgacatccgc gtagtattgc agcaagtccc gcttggtcag
     9181 gccgagttca ggccagaaaa cctttctcaa gttagttagc gagacgcgcc gccccgccac
     9241 ttccagtacg gccgcgtttt cgtccgggat ttcgacccgt ggcgtgatcc gggttctctc
     9301 agcgcgagcg gtcgccgtct tggacttgac gccacgactg gcaaaacgtg cacgcggcat
     9361 gcgcggacta ctctaccggt tcgctcgacc tcgggtcttc tccgggcgac cgcgcggccg
     9421 ccggcaccgg ctccgcttcc ggctcgggcc agggcccgcc agattcatcc agggcgtcgg
     9481 cctcagtttt ttcacgaatc cgctcccgcg tttcggg
//
LOCUS       DAOSYS010000142         9488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6167_length_9488_cov_5.016856, whole genome shotgun sequence.
ACCESSION   DAOSYS010000142 DAOSYS010000000
VERSION     DAOSYS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6167_length_9488_cov_5.016856"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PLU99_09765"
     CDS             <1..498
                     /locus_tag="PLU99_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17384.1"
                     /translation="LSRCYQLDFQAFLLLAGKLLGAALALGLALVLGAVFFGHFVLEA
                     LYRAEYAAFLPEFMIIILAQSLMLVTIVFGVMTTQMRLFWLQVPAQLTVLVCTTVAAL
                     LLIPSAENLVRGGAWTMLVRALVHVSLYAACVAFGIVFRSRVLAGRVNLTPRAGGTEG
                     ISQDW"
     gene            complement(569..643)
                     /locus_tag="PLU99_09770"
     tRNA            complement(569..643)
                     /locus_tag="PLU99_09770"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(606..608),aa:Asp,seq:gtc)
     gene            complement(860..>2113)
                     /locus_tag="PLU99_09775"
     CDS             complement(860..>2113)
                     /locus_tag="PLU99_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17385.1"
                     /translation="ALDNEARLWARGLEGFNLAIRFPHVFALINAHAKVDESKNPPIV
                     RGDRIEFALIVETRGHDEPFARVIQAAINEQTDREKAHLERRQAEGWSYMELRDERLP
                     EYCRLAWGNIGPFFVFTVGQDVWPRVAAVAAGRQEALSHTDWLEGVRGPRARQALIEI
                     IVHSQDIRGRLDPFVDGRATAFFSAWQADDIERSHWALGFEGRAMYCLANFVENGQPR
                     QRLLADPNVDDPVLLATIPEGARYAVYRVSPAETLPRFFNGLLATRDARERRTIRRLW
                     KQIQEERGFDADRDILAHLGEHIVMHNDPPHPLHIPLAMTTLTEIRDEPAQVSRAIDA
                     LCEAWRDAQQKFEAQLKAQNSDGELPPSMIIDRDTTGIWYMKFGLFAGPAWVVTDRYL
                     ITSWSSEALRAYLDKVGERAGRRPR"
     assembly_gap    2114..2213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2214..2616)
                     /locus_tag="PLU99_09780"
     CDS             complement(<2214..2616)
                     /locus_tag="PLU99_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17386.1"
                     /translation="MQYKFGIAAGAALVVVLAAWSWREPDRPASSSAPSLPSAAVAPQ
                     EPAQASPATSADPGSTDRSAPSSTRPVSPPSECLDLVPAETLLCWYGRPFPDMAPPTD
                     GPSPMRLLLDLFGHALDNEARLWARGLEGFNL"
     gene            complement(3146..6790)
                     /gene="smc"
                     /locus_tag="PLU99_09785"
     CDS             complement(3146..6790)
                     /gene="smc"
                     /locus_tag="PLU99_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOO17387.1"
                     /translation="MYLKRIVLHGFKSFADRTEFDFGPGVTAVVGPNGCGKSNLLDAV
                     RWVLGEQSARSLRGKRMSDVIFAGSRSRKPAAAAQVELVFDNSAGFLATDEPEVSISR
                     VLYRSGDSDYRMNGNSCRLKDIRQLFLDTGVGVDAYSIIEQGRVDMLLQADPMQRREI
                     FEEAAGISRYKQQRAEAQRKLERSQNNLLRLNDIIDELERQLRSVKLAAGKARRWQEY
                     DRRLRELRASFSLAEYHELETGCAGVRAQMAEHSDRLQAERATLAAHDAAAAQHERDL
                     QQLDERIQHTDAELAALHGELSTLSERIAQSERRAADLAAARERRHSQSAEISQQLAE
                     LEQRIVAESAALDVLAQAAERSAGRIVQLQRARSEVEQRRDGARQAVDQARAAVFDAA
                     RTAALLNNEQNNLVAQRERLNSQSDRLKARRAELEQEQNELGQRQARALGEVGRLDQR
                     VAELSQQIRDAEAQVAQLRSTRQAHEAEIGAAKERRSALLSRLELLEDMERRLEGVDG
                     GTQAVLAWRDEGDERAGTIRGLVADLLHIDDPRVQALQPVLATFENHVVVEDSYAFLT
                     ELNRRPELSGPVRVIALDRLPEDFSRGHYDESPGFVARASTWVQCAPQYRKLAEHLLG
                     RVIVVDSLERALALANGAPQGYVFITLDGRSVSTDGRITIAGAQAGAGLISRKAEVRH
                     LQQERDEVETALERCVRQRNEVDQQLSDEQLRLTSLMNEIAAAQREHAEARSTLTRLE
                     GELARLRREAEVSHSELEAIHRALVEMEELARKLAAQFEAAGQDQQERQSRIEVLERE
                     LAELEAAVSSAAAELTEARVEAGRNSEKLAASAEAFAQLQAGRAGLQRDLERAAQEIE
                     QAAAQIAQSQAEAEAARTRQTECAAQLELLRAQTGELRQCRQELRARIEEFGALARGT
                     TEQIHQIESQIHAAEVALREFEVRRENLVNRVRDELNIDLAAAYQEQQEQRRAAEMAA
                     AAAAAEGAAVEAPPAEQDWEAVRAEIAELREKLARLGNVNLDAITELEELTPRYENLV
                     AQRTDLLASIERLQALIAELDQESQTRFATAFNQIREYFQELFRQIFGGGKADIVLLD
                     PEKPLECGIEIIARPPGKEPQSLSLLSGGEKTMTAVALVMAVFKSKPSPFAFLDEVDA
                     ALDEANTERFNNVLQGFLAHSQFVVITHSKRTMSSADVLYGVTMEEPGVSKRVSVRFE
                     NGRVQTPNVA"
     gene            7086..9056
                     /locus_tag="PLU99_09790"
     CDS             7086..9056
                     /locus_tag="PLU99_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17388.1"
                     /translation="MDPLKIGHVISVNGDSVEVQITVSNLALEYHGTTYRVGRLGTYV
                     TLPLGRIMLIGYITRVGAAGDLEPLPDPGAPRRITMTVQLLGTVRNDKFSRGVNEYPT
                     LGDPVRLGVDEDFELIFGCFDQIAGSGTGPKSFPMGRFAVDTDFEVKVVGKDFFAKHV
                     AVMGNSGSGKSVTTAKILHEAMQLPHSHIVLFDMHGEYLNAFSDERGQPLPNVTYLSD
                     RNLVLPYWMLQYQELEALFVDTNNEANINPQNVFLRAALDKMKRPAAAELGLLREYTV
                     DTPVYYSIEQLKTYAENMNDARLVLGSEHYAFSRLPYRQLPVEKQEEMLLTQRMEFNR
                     GNAEGEVPHATYYGRLLGLVNQLETRMNDRRYDFLLRPFEQAQRNPELAGCLSKAASP
                     GQSSKAVAAVIRQLLGRQGTQKNLTIVDLSGLPFDVVDITVAVLTRTLFDFNFWSPPE
                     VRQPFVLVYEEAHNYLPRRDRRGRMFARDAVEKVAKEGRKYGVSAMVVSQRPSEISET
                     ILSQCNSMVLMRMNNPDDQNYAARVVSDQFRSLISLLPSMRPGEAFIIGDAVLMPMRT
                     LIDMPPKLPRSGDVDFFGLWARGTASSEDERDAAQVDQIIDRWWRQDRSAVNAGAPGS
                     SQEPAPASSGQAPKSNAERKIAELAAMLNAMD"
     gene            complement(9094..>9488)
                     /locus_tag="PLU99_09795"
     CDS             complement(9094..>9488)
                     /locus_tag="PLU99_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOO17389.1"
                     /translation="IALCRAAGLPARYQGSVVVRGDDASVDEAFHRWAQVYLPNYGWV
                     PVDADKGDAASPVDRARGFGELANRFLITTHGGGDSEYLKWGYNSYAHYKMTGYCKVE
                     EDNIGIWEPLAPAEAQPQPSSAGSGTKE"
BASE COUNT         1584 a         3094 c         2984 g         1726 t
ORIGIN      
        1 ctgtcgcgct gctaccagct cgattttcag gcctttcttt tgctggccgg caagctgcta
       61 ggcgcggcgc tggcgctggg cctggcgcta gtgttgggcg cagtgttttt cggacatttc
      121 gtgctcgaag ccctgtatcg tgcggagtac gccgcgttcc ttccggaatt catgatcatc
      181 atcctggctc agagcctgat gctggtgacg attgtgttcg gcgttatgac cacgcaaatg
      241 cgattgttct ggcttcaggt gccggcgcag ctcacggtgc tggtttgcac gaccgtggcg
      301 gcgctgctgc tgatcccctc ggcggaaaac ctggtgcgcg gcggcgcctg gacgatgctc
      361 gtgcgggccc tcgtgcacgt cagcctctac gcggcctgcg tggccttcgg aatcgtgttc
      421 cgctcgcgcg tgctggccgg ccgtgtgaat ctcacgccgc gcgcgggcgg aacggagggg
      481 atatctcagg actggtagag accagggcgt ttagcaaaaa aaagcgcccc ggcttggata
      541 caggggcgct ggcgttgcga ttgggaaacg aggccgacgg ggctcgaacc cgcaacctcc
      601 gccgtgacag ggcggcgctc tgaaccaatt gagctacggc cccagtcgca tctgagccgc
      661 aaaaacgcaa ttttacctca cacgccattt ctgtcaacga tcgaaaccgg gtaaccggag
      721 ttatcgcctg gaatctgagg ctttcgctcc gcgctgtcca ggccgcggct taggatatgg
      781 gaccggacac gaaatatcag gccgtcgcgg gcgaagagcc tgggggcgcg tcgagggctg
      841 cgtggtggag gcaggctccc taacgtggtc ggcgcccggc gcgttcgcca accttatcga
      901 ggtaagcgcg cagcgcctcc gatgaccaac tcgtgatcag atagcggtcc gtcacaaccc
      961 acgccggccc ggcaaacagg ccgaatttca tgtaccagat cccggtggtg tcgcggtcga
     1021 tgatcatcga gggcggtagt tcgccgtccg aattctgcgc ttttagttgc gcctcgaatt
     1081 tctgctgcgc gtcgcgccaa gcctcacaca acgcgtcgat agcccggctt acctgtgccg
     1141 gctcgtcgcg aatctccgtc agcgtggtca tcgccagcgg aatgtgcaac gggtgcggcg
     1201 ggtcgttgtg catgacaatg tgctcgccca aatgggccag gatgtcacga tcggcgtcga
     1261 agccgcgctc ctcctgaatc tgcttccata gccgccggat agtgcgacgc tcgcgcgcgt
     1321 cccgcgtggc caacaggccg ttgaagaacc gcggcagcgt ttcagccggg gagactcggt
     1381 agacggcata acgcgccccc tccgggatgg tggccagcag cacggggtca tcgacgtttg
     1441 gatcggccag caagcgctgc cggggctgcc cgttttcgac gaaattggcc aggcagtaca
     1501 tcgcccggcc ttcgaagccc agcgcccaat gcgagcgctc gatgtcgtcg gcctgccagg
     1561 cgctgaaaaa cgccgtggct cgtccatcca caaacgggtc caaccgcccg cggatgtcct
     1621 gcgagtgcac aatgatctcg atcagcgcct gccgcgcccg cgggccacgc acgccctcca
     1681 gccaatccgt gtgactgagc gcctcttgcc gcccggccgc caccgctgcc acgcgcggcc
     1741 acacatcctg cccgacggtg aagacgaaga acgggccgat gttaccccag gcgagccgac
     1801 aatattccgg caggcgctcg tcgcgcagtt ccatgtacga ccagccttcc gcctgccgcc
     1861 gctccaggtg agccttctcg cgatcggtct gctcgttgat cgcggcctga atgacgcgcg
     1921 cgaagggttc gtcgtggccg cgggtctcga cgatgagggc gaactcgatt cgatcgccgc
     1981 gtacgatcgg cgggttcttc gactcgtcga ccttcgcgtg cgcgttaatc agtgcgaaca
     2041 cgtgcgggaa gcggatagcg agattgaagc cttcgaggcc gcgggcccac aggcgggcct
     2101 cgttgtccag cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgagatt
     2221 gaagccttcg aggccgcggg cccacaggcg ggcctcgttg tccagcgcgt ggccgaataa
     2281 gtccaacagg agccgcatgg gcgagggacc gtccgtggga ggcgccatgt cggggaaggg
     2341 gcgtccgtac cagcacagca atgtctcggc gggtaccaga tcgaggcatt cagacggcgg
     2401 tgacacgggg cgggtggagc tcggcgccga acggtcggta ctgcccggat cggcgcttgt
     2461 tgccgggctg gcctgtgccg gctcttgcgg ggcaacagcg gccgacggca agctgggcgc
     2521 cgatgaagag gccgggcggt ccggctcgcg ccatgaccag gcggccaaaa cgaccaccaa
     2581 tgccgccccc gccgcaatcc cgaatttgta ctgcatctgc tcagctccgc tggggccgat
     2641 ctctgaagtc gggcagatta cccgcgccgt gcggtcgttg ccaacgatct gccctgcgcg
     2701 gcgtgtcgaa atctgtgcac ttcgccaccc ccgaaagtgg gaaggtggga aagtgggaag
     2761 gtgggaaggt gccggccact ttcccacctt cccacattcc cactcacacg cggccgtaat
     2821 cgcagacgtc gccaagtatc cgcgcaaaca aaggcggcct gcccagtaca ccgtcgcgaa
     2881 actcaccacc ggctcttgac cggctgggtc ccgcgacccg cgccttgcgc gtctgctgta
     2941 cagggcaggc cgcttgaaga ccgaataagc caaatgctcc ggtcaggccg tcggggcggc
     3001 ctcgaccggg cgagaaagct acatggcctt cttcttgctc gtcttcttgg ctttcttctt
     3061 ggcggccttc ttcgtggcct tcttctttac gaccttcttc tttgccatgg cgcaaacctc
     3121 acaaaaaaga acagaacaga aacactcacg ccacattggg cgtttgtacg cgaccgtttt
     3181 cgaaccggac gctcacgcgt ttgctgacgc ccggctcctc catggtcacg ccgtacagca
     3241 cgtcggcgct cgacatggtg cgcttgctgt gcgttatgac cacaaactgc gaatgcgcca
     3301 agaagccttg tagtacatta ttgaagcgtt ccgtgttggc ttcatcaaga gccgcatcca
     3361 cctcatccaa aaaagcgaat ggtgaaggct tactcttgaa cacggccatg accagcgcca
     3421 ccgcggtcat ggtcttctcg ccacccgaga gcaaggatag tgattgcggc tcttttccgg
     3481 gcgggcgggc gatgatctcg atgccgcatt ccagcggctt ttccgggtct agtagaacga
     3541 tgtcggcttt gccgccgccg aagatttggc ggaacagctc ctggaaatac tctctgatct
     3601 ggttgaaggc ggttgcgaag cgcgtctgcg attcctggtc gagctccgca atcaacgcct
     3661 gcagacgttc gatcgacgcc agcaggtcgg tgcgctgcgc caccaggttt tcatagcgcg
     3721 gcgtcaactc ttccagctcc gtgatcgcgt cgagattcac attgcccaga cgcgccagct
     3781 tttcacgcag ctccgcaatc tccgcccgca ctgcctccca gtcctgttcg gcaggcggcg
     3841 cttccacggc cgcaccctcg gcggcggccg ccgcggccgc catttccgcg gctcggcgtt
     3901 gctcctgctg ctcctggtaa gccgcagcca gatctatgtt caactcgtcg cgcacacggt
     3961 tgacgaggtt ctcacgccgc acttcaaact cgcgcagcgc cacctcggcc gcgtgtatct
     4021 gcgactcgat ctgatgaatc tgctcggtcg tcccccgcgc caaggccccg aattcctcga
     4081 tccgcgcccg cagttcctgt cggcattgcc gcaactcgcc ggtctgggca cgcaacaact
     4141 cgagctgcgc cgcgcactct gtctgacgag tgcgggccgc ttcggcctca gcctgcgatt
     4201 gggcaatctg ggcggcggcc tgctcaatct cctgcgcggc gcgctccaaa tctctttgca
     4261 gaccggcgcg cccggcctgg agctgcgcga acgcctcagc gctggcagcc agcttttccg
     4321 agttgcgccc ggcttcgacg cgggcctcgg tcaactcggc cgctgcactg ctgaccgcgg
     4381 cttccagctc ggccagctcg cgttcgagta cttcgatgcg tgactggcgc tcctgctggt
     4441 cctgtccggc ggcctcgaac tgcgccgcaa gtttgcgggc cagctcttcc atttccacca
     4501 acgcgcggtg aatcgcttcc agttcgctgt gtgaaacctc ggcctcacgc cgcaggcggg
     4561 ccaattcgcc ctccaggcgc gtcagggtgc tgcgcgcttc cgcgtgttcg cgctgcgcgg
     4621 ccgcaatttc gttcatcagc gacgtgagcc gcaattgttc gtccgagagc tgttggtcga
     4681 cctcgttgcg ctggcgcacg cagcgttcca gggccgtctc gacctcgtcg cgctcctgct
     4741 gcagatggcg aacctcagct ttgcggctga tcaggcccgc cccggcctgg gcgccggcga
     4801 tggtgattcg gccgtcggtc gaaacggagc gcccgtcgag ggtaatgaag acgtagccct
     4861 gcggggcgcc gttggccaaa gctagggcgc gttccagcga gtccaccacg atcacgcggc
     4921 ccagcaagtg ctcggccagc ttccggtact gtggggcaca ctgcacccaa gtggaagccc
     4981 gcgccacaaa cccgggcgat tcatcgtaat gtccgcggct gaaatcttcc ggcagccggt
     5041 ccagcgctat gacccgcacc ggaccggaca attccggccg gcggttgagt tccgttaaaa
     5101 acgcgtacga gtcttcaacc acgacgtggt tttcaaacgt ggccagcaca ggctgcagag
     5161 cctggacccg cggatcatcg atgtgcagca ggtcggccac gagcccgcgg atagtgccgg
     5221 cccgctcatc gccctcgtct cgccacgcca gaaccgcctg cgttccgccg tcgaccccct
     5281 ccaggcggcg ctccatatcc tcgagcaact cgagtcgcga cagcaaagcg ctgcgccgct
     5341 cctttgcggc gccgatttcc gcctcatgcg cctggcgcgt gctccgcaac tgcgctactt
     5401 gcgcctcagc gtcacgaatc tgctggctca actctgcgac gcgctggtcg agccggccta
     5461 cttcacccag cgcacgcgct tgccgctgac caagctcgtt ctgttcctgc tccagctcgg
     5521 cgcgccgggc cttcaaacgg tccgattgcg aattcaggcg ctcgcgctgc gcaacgaggt
     5581 tgttctgttc attgttcagc agggccgccg tgcgcgccgc gtcgaacacc gcggcccgcg
     5641 cctgatccac cgcctgccgc gccccgtccc ggcgctgctc gacttcgctc cgcgcccgct
     5701 ggagctgcac aattctcccg gcgctgcgct cggccgcctg cgccagaacg tccagcgcgg
     5761 ccgactcagc cacgatgcgc tgctcgagct ccgcaagctg ctgtgaaatt tcggccgact
     5821 ggctatgccg gcgctcgcgg gcggcggcca ggtcagcggc gcgcctttcg ctctgcgcga
     5881 tacgctcgct gagcgtgctc aattcgccgt gcagggccgc caattcagcg tcggtatgct
     5941 ggattcgttc atcaagctgt tgcaggtcgc gctcgtgctg cgcggccgcg gcatcgtgcg
     6001 ccgccagggt ggcacgctcg gcttgcaggc ggtcgctgtg ttcggccatt tgggcccgaa
     6061 ccccggcgca gccggtttcc agctcatggt attcggccaa cgagaaggaa gcccgcagtt
     6121 cgcgcaaccg gcgatcgtac tcctgccagc ggcgggcctt tccggccgcg agcttcacgc
     6181 tgcggagctg ccgctcgagt tcgtcgatta tgtcgttgag gcgcagcagg ttgttttgcg
     6241 agcgttcgag cttgcgctgc gcttcggcgc gctgctgctt gtagcggctg atgccggccg
     6301 cttcctcgaa gatctcgcgg cgctgcatcg ggtcggcctg gagcagcatg tccacgcggc
     6361 cctgctcgat gatgctgtag gcgtcgaccc ccacgccggt atcgaggaac agttggcgga
     6421 tgtccttcag ccggcacgaa ttgccgttca tgcggtagtc gctatcgccg ctgcggtaga
     6481 gaacgcggga gatgctgacc tcgggttcat cggtcgcgag aaatccggcg ctgttgtcga
     6541 acacgagttc gacctgcgcc gcggccgcgg gcttgcgcga acgcgaaccg gcgaagatca
     6601 catcggacat gcgcttgccg cgcaggctgc gggccgattg ctcaccgagc acccagcgca
     6661 ccgcgtccag cagattgctc ttgccgcagc cattcgggcc gacgacggcc gtcacgccgg
     6721 ggccgaagtc gaactccgtc cggtcggcga agctcttgaa gccgtgaaga acaattcgct
     6781 ttagatacac gattgacgcg attatgggcg tcttttgcac agggccaagg ccccgcatcg
     6841 gatcgccccg ccgggccgcg ccttcagcct tcaacgacgg gacgccacca caacatgtgg
     6901 tgcaacgtta gcgagattac cacaacaact tcgccacggc aaggggtagg cagctttccg
     6961 tcatcagttt tgcgtcgcta gtcgccaagt ttcaggtgtc gggtgagcac gcacaactga
     7021 aaactggaaa ccgaaaactg gcaactcccg attgagttta agggccccaa tgccggagca
     7081 cacgcatgga tccactgaag atcggacacg tcatcagcgt taacggcgac tcggtcgagg
     7141 tgcagataac tgtctcgaac ctcgccttgg aataccacgg aaccacctat cgcgtgggcc
     7201 ggctcggcac gtatgtcacg ctgccgctgg gccgcatcat gctgatcggc tacatcacgc
     7261 gcgtcggggc ggccggcgat ttggagccct tgcccgaccc cggcgctccg cggcgcatca
     7321 cgatgaccgt gcagcttttg ggaaccgtgc gcaatgacaa gttctcgcgc ggcgtgaatg
     7381 aataccccac gctcggcgac ccggttcgcc tgggcgtcga cgaggatttc gagctgatat
     7441 tcggctgctt cgatcagatc gccggcagcg gaaccggccc gaagtccttc ccgatggggc
     7501 gcttcgcggt cgatacggat ttcgaagtca aagtcgtagg taaggacttc ttcgccaagc
     7561 acgtcgcggt gatgggcaac agcggatccg gcaagagcgt caccaccgcc aagatcctcc
     7621 acgaggccat gcagttgccg cactcgcaca tcgtgctgtt cgacatgcac ggcgagtacc
     7681 tgaacgcgtt cagcgatgag cgcgggcagc cgctgccgaa cgtgacctac ctgtccgacc
     7741 gtaacctggt gttgccctac tggatgctcc agtaccagga actcgaggcc ctgttcgtcg
     7801 acacgaacaa cgaggccaac atcaaccccc agaacgtgtt cctgcgggcg gcgctggaca
     7861 agatgaagcg ccccgcggcc gccgaactgg gcctgctgcg cgagtacacc gtcgatacgc
     7921 ccgtgtacta ctcgatcgag cagctcaaga cgtacgccga gaacatgaac gatgcccggc
     7981 tggtgctggg ttccgagcac tacgcgttct cgcgcctgcc ttatcggcag ttgccggtgg
     8041 agaagcagga agagatgctg ctgacgcagc gcatggagtt caaccgcggc aacgcggaag
     8101 gcgaggtgcc gcacgccact tactacgggc ggctcctggg tctggtgaac cagctcgaaa
     8161 cccgcatgaa cgaccgccgt tacgactttc tattgcggcc gttcgagcag gcgcagcgta
     8221 acccggaact ggcgggctgc ctgagcaagg ccgcctcgcc ggggcagtcg agcaaggcgg
     8281 tcgcggcagt gattcggcaa ctgctgggac ggcagggcac gcagaaaaac ctgacgatcg
     8341 ttgacctttc gggtctgccg ttcgacgtag tggatatcac ggtggccgtg ctgacgcgca
     8401 cgttgttcga cttcaacttc tggtcaccgc cggaggtgcg gcagccgttc gtgctggtgt
     8461 acgaggaggc gcacaattac ctgccgcggc gtgatcggcg tgggcggatg ttcgcgcgcg
     8521 acgcggtcga gaaggtggcc aaggagggcc gcaaatacgg ggtgtcggcc atggtggtgt
     8581 cgcagcggcc gagcgagatt tccgaaacga ttctgagcca gtgcaactcg atggtcctga
     8641 tgcgcatgaa caacccggac gaccagaact atgccgcgcg ggtcgtcagc gatcagttcc
     8701 gcagtctgat cagcctgctg ccgagcatgc gtccgggcga agcgtttatc atcggtgatg
     8761 cggtgctgat gccgatgcgc acgctgattg acatgccgcc gaagctgccg cgcagcggag
     8821 acgtggactt cttcgggctg tgggcgcgtg gcacggcctc gagcgaggac gagcgcgacg
     8881 ccgcgcaggt ggaccagatc atcgaccgct ggtggcgcca ggaccgcagc gccgtgaacg
     8941 cgggcgcgcc gggcagcagc caagagccgg cgccggcgag cagcgggcag gcgccgaagt
     9001 ccaacgcgga acgcaaaatc gccgagttgg cggccatgct gaatgcgatg gactaggaaa
     9061 acggcaaccg gcctcgcctt cgccggccgc ctcctactcc ttggtcccgg aaccggcgga
     9121 tgacggctgc ggctgcgcct cggccggtgc gagcggctcc cagatgccga tgttgtcctc
     9181 ctcgaccttg caatagccgg tcatcttata gtgtgcgtag gagttatagc cccacttgag
     9241 gtattccgaa tcgccgccgc catgcgtcgt gatgaggaag cggttggcga gttcgccgaa
     9301 gccgcgggcg cggtcgaccg gtgaggccgc atcccccttg tccgcatcca ccggcaccca
     9361 gccgtagttg ggcaggtata cctgggccca gcggtggaag gcttcgtcga cgctggcgtc
     9421 atcgccgcgg acgaccacgc tgccctgata gcgggccggc aggccggcgg cgcggcacag
     9481 ggcgatga
//
LOCUS       DAOSYS010000143         9264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6351_length_9264_cov_5.001086, whole genome shotgun sequence.
ACCESSION   DAOSYS010000143 DAOSYS010000000
VERSION     DAOSYS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6351_length_9264_cov_5.001086"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1113)
                     /locus_tag="PLU99_09800"
     CDS             complement(226..1113)
                     /locus_tag="PLU99_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17390.1"
                     /translation="MKIAGQLGRTIEPGATEEIQVTVPAQTGDINRKIRVQTNDPNNA
                     NVELTCKGKILVPFRTDPATINFGKIGRDSGPVTKTVNLTRGDGGPLNPKIGKMDSSL
                     SAELREIEPGEKYALDITVSPPWPNDKLRGSVTIETGLEEAPTETVRLFGQVDPRLKT
                     EPKSFVLPADPPEDLDLRVALVWDGPPGKAIGATATDQTLKAELIEEDGKQFVSLHVP
                     AHYQRKQKMALSVNVTTDDPAVSSLRIPISATGAAADKEIAQPARLAPVAKPGNAATP
                     GPELQQPASRPAHLQAPSH"
     gene            complement(1117..1704)
                     /locus_tag="PLU99_09805"
     CDS             complement(1117..1704)
                     /locus_tag="PLU99_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17391.1"
                     /translation="MRVLEELRKSGGWQARVLPIATLAVAVALAGAAYAQQTNAKPNM
                     GGGAAATTKPAAAGNVEGQEAEGLKRLIQQKMQEQGLDSNVARPPASPSVRQPSRPAA
                     LSPKRVQLSKEAVPTNGPAGNRAPETQPAKGCSAGGKVQVDLTPPPPDQPQPRWSVVQ
                     DTIEVPPLWKGSKAEFVFQVRNAGEGPLNIQLKGG"
     gene            1939..2952
                     /gene="ilvC"
                     /locus_tag="PLU99_09810"
     CDS             1939..2952
                     /gene="ilvC"
                     /locus_tag="PLU99_09810"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOO17392.1"
                     /translation="MTKTDKIPALTADARPELLSGCNVAVLGFGAQGHAHALNLRDSG
                     VNVVVAQRAGASHDSAVAAGFSPITIDKAVQTADLLIFGIPDELAPEIYRQQIAPHLR
                     PNQTLGFIHGYNIHYRHITPPPNLDVVLVAPKAQGRAVRSEFLAGRGVAALVAVHQDA
                     TGRAQQTALGWAAGLGCHRTAILKTTFADETETDLFGEQAVLCGGLVGLIKAGFDTLV
                     EAGYPPELAYFECCHEVKLIADLIYEHGITGMRERISNTARFGDLTRGPRVIGAATRQ
                     AMREVLAEVRSGQFAREWADQTQQGQPEFRRLTDADRGHLIEQVGATLRKLMWGQSPQ
                     APH"
     gene            3131..4978
                     /locus_tag="PLU99_09815"
     CDS             3131..4978
                     /locus_tag="PLU99_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOO17393.1"
                     /translation="MLRIISLVVLFTAAVAPADTIYVNATLGSDAWDGRCEYWDGQSC
                     GPKATIQAGIDAAAEHDEVLVAEGIYSGPGNRDLDLYGKALTLRAAGGEPGDCVIDCE
                     HSGRAFYFHSGEGADSIVAGFLIRHADGDAGTIYCYGGASPTLVDCTITNNTANNGGG
                     LFCDEGSRPTLTRCVIANNLANCGGGVACKSYSDATLVDCTVADNLGAYGAGVWCSDH
                     SNVTLVDCIISGNANGDFGAGVDCYDSDPTLINCVISGNSTTYAGGGLHFYHSNGLLV
                     NCSISNNDGGDSAGGIYSRYDSAPTLIDCLIAGNTSSNCGGGVYCHYATAYMINCTLS
                     GNQARFGGGLDCLPDSSPVLTNCYIFGNTATARGGGMRCYESDPILNNCIIAHNSASE
                     SGGGLYCEYQSNAVLNNCTITGNTAGTGGGLACNQESAPSLTNCILWGDTPSEIFLDG
                     GAPMVTFCDVQGGWSGTGNIHADPLFRDADGPDGDPHSWHDNDYRLASGSPCIDAGDP
                     GFAPEAGATDADERTRIWDGDGNGQPRVDMGAYEFGSFRFGDVNCDGSINVFDIDPFV
                     LALVDPAAYAEEYSGCDASLADLDHDGHINCFDIDPFVGVLTAGDSGDL"
     gene            complement(4973..5692)
                     /locus_tag="PLU99_09820"
     CDS             complement(4973..5692)
                     /locus_tag="PLU99_09820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO17394.1"
                     /translation="MTTLQQRLGRPARRWLRHFYYNPRLSFLTRVLQPAGKHEQQSYW
                     DAALSGHMVDYLGDTRAVIQSNAIVVGVLRAYGPPLRRVLDVGCAGGTLAYDLPADVD
                     YLGIDISAVAIADALQRRRQRAARPGRTEFQHSSIADFQFDAGSEFDAIVLSEVVYYL
                     TPQVALEQTLRYSAALSPGGVLVVSMKQDPKSLLIFRQMERALVWLGGVLYQPKSVID
                     FRVRPNGKSPAFLVGAFRPKS"
     gene            6076..7803
                     /locus_tag="PLU99_09825"
     CDS             6076..7803
                     /locus_tag="PLU99_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HOO17395.1"
                     /translation="MHRYLQTAACGLCILLAGAAFAQQVGEEAPDLKLPKSEHATSEK
                     DLISLFELYPHRILVLYYWSSLDSASLDFFSTLDELEEKYGPRGVTILGLTRQHKKVM
                     EKVAKERKPKFGLLQLSPTMGTFRDYRRIWRFPAPGVAYIIDTHRRVAYARFDATDPR
                     EKLEEKIQTVLAKTPTLAATEELLQKNIQKVSNFIEQGEFGRAYTLGTALKEFFRDRE
                     DSALEEQAEELLRQISLGAHRQLESAQQDFKEEKYEDAAAKLAELSVRLTGLGDGGDK
                     RGRRRSSRSTRSGARRSPRGGQGVEPGKDFREIKENVEREIGRMQSENYTKAIILKAL
                     DNARGQVRNDSAAECMEFGQYSIARRLYNSVVEEFSDTEAAEAAQEALDRIENDPRIQ
                     EAIAKSEASEQAERWYDLGDRFARAEMFDKSREYFEKVIKGYPDSAAAQSARDRLARM
                     EDDEKAAGEGRQKREKLVPPKDDTKIVEQARTILQKLIDGDYRGFVEAGDDQLRASLS
                     EQKARQLWADLRTKLGEFKSVDQGKLTSILGLRAVTFVAQFERGERRLQIVFSTQGKM
                     TEFKLLVEP"
     gene            complement(7866..>9264)
                     /gene="murD"
                     /locus_tag="PLU99_09830"
     CDS             complement(7866..>9264)
                     /gene="murD"
                     /locus_tag="PLU99_09830"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOO17396.1"
                     /translation="VTRWLARAGARLTLTDKLPAAELGDSLAQLDGLEINYRLGGHDE
                     RDFRETDLVVINPAVPDTSPFLQAAVSAGVPLTTEINLFVERCRARCVGVTGSVGKST
                     VTAMVGHVLREGIEGSRDQGIRGSRDLGIDSEGSCSAFDASGGGRRVWVGGNIGRSLL
                     EVLEEIRTEDIVVLELSSFQLQRTPLVAWSPHIAVLTNISPNHLDWHGNFAGYLAAKL
                     NIVRFQDPARDAVVIGDDPELQRHFDRLFGDLSGVWRYGLHGDDPIAVCQSTAAVDCD
                     DRRLRWPDTRLAVPGRHNRLNAAAALTVAHLLGIEPQRSRAALATFEALPDRLQRVGE
                     IDGVTYYNDSKSTTPTAAATAMQAIDGPLLMILGGYDKGSDFGEFARAAAIRLKYAAC
                     IGQTGKQIAELIRTAGGQAEYCGDLASAVSACRAQAAAGDTVLLSPACASWDQFKDYR
                     ARGAAFSRLIRGGAD"
BASE COUNT         1756 a         2979 c         2860 g         1669 t
ORIGIN      
        1 cagggggctg gtcatcgaag ccggcaagac gctgatcctt gagcgcgagc gcctgctcga
       61 actcgctgga aagagcatta gcgtgatcgc gcgttagccc cggcgatcgg tccggcggca
      121 gcttaaagag cgcgaccgca gtgcttctgt tcactgcggt cgcggctagg ccaaatacgc
      181 gcaaggccgg cttgctctca tgaacaagcc ggcctgcaaa ggccgctaat ggctgggcgc
      241 ctgcaggtgc gccggccggc tcgccggctg ctgcaactcc ggaccgggcg tcgcggcgtt
      301 ccccggcttg gccaccggcg cgagcctggc cggttgggca atttccttat ctgcggccgc
      361 cccagtcgcc gagatgggga tgcgcaggct gctcaccgcc ggatcgtccg tggtcacgtt
      421 aaccgaaagg gccatcttct gcttacgctg gtaatgggcg ggcacgtgga gcgacacgaa
      481 ctgcttgccg tcttcctcga tcaactccgc cttgagcgtt tggtccgtgg ccgtcgcacc
      541 gattgccttg ccgggcgggc cgtcccacac cagggccacg cgcaggtcga gatcctccgg
      601 cgggtccgct ggcagcacga agctcttggg ctcagtcttg aggcgtggat cgacctgtcc
      661 gaagaggcgg accgtctccg tgggggcctc ctccagcccg gtctcgatgg tcaccgagcc
      721 acgcagcttg tcattcggcc acggcggact gacggtgatg tcgagtgcgt acttttcgcc
      781 gggttcgatc tcacgcagct ccgccgatag cgaagagtcc atctttccaa tcttcgggtt
      841 cagtggtccg ccgtcgccgc gcgtcaagtt gaccgtcttg gttaccggtc ccgaatcgcg
      901 cccgatcttg ccaaaattga tcgtggctgg atcggtgcgg aagggcacga gtattttgcc
      961 tttgcaggtc aactccacat tcgcgttgtt cggatcgttc gtctgcaccc ggatcttgcg
     1021 gttgatgtcg cctgtttgag cggggactgt gacttggatc tcttcagtcg cacccggctc
     1081 gatcgtccgg cccaactggc cggcaatctt caaccctcag ccccccttca gttgtatgtt
     1141 cagcggtccc tcgcccgcgt tcctcacctg aaacacgaac tcggccttgc tgcccttcca
     1201 cagcggcggg acctcgatgg tgtcctgcac gactgaccag cgcggctgcg gctgatccgg
     1261 gggcggcggc gtcaggtcaa cctgaacctt tccacccgcc gagcagcctt tggcaggctg
     1321 agtttccggc gcacggttcc cagccgggcc gttggtcggc acagcttctt tcgacaattg
     1381 aacgcgcttc ggactcagcg cggcgggccg cgacggctgc cggaccgacg gcgaggccgg
     1441 tggacgtgcg acgttggagt ccagcccctg ctcctgcatc ttttgttgga tcaagcgctt
     1501 gagcccttca gcctcctgcc cttcaacatt tccggcggca gccggtttcg ttgttgcagc
     1561 cgcaccaccg cccatgttcg gtttcgcgtt tgtctgctgg gcataggcag cgccggccaa
     1621 ggcgacggcc accgctaacg tagcgatggg caggacccgc gcctgccaac cgccgctctt
     1681 cctcagctcc tctaatactc gcatatgagt tttccttacc aaaaagcctc acacacacgg
     1741 ctcgtggttg ctgcttctgg agatcggctg cagtaaacca cggaaacccg tcatcatatc
     1801 cttaatcgcg tttgctggaa gcccaaaact tgcgcaatct ccgtacaatc aaggtccgcc
     1861 gggcctagac acgcccggac agcatacaag acacgatggt ggcccactgg ttagctgctc
     1921 aggattcgga atccgcccat gacaaagact gataagatac ccgcattgac ggccgacgcc
     1981 cgccccgaac tgctttctgg atgcaacgtg gctgtcctcg gcttcggggc tcaggggcat
     2041 gcacatgctt tgaatcttcg cgattcgggc gtgaacgtgg ttgttgcaca aagagcgggc
     2101 gcgtctcacg atagcgccgt agccgcggga ttcagtccca taaccatcga caaagccgtt
     2161 caaaccgctg acctgctgat cttcggaata cccgacgagc ttgctcctga aatttatcgc
     2221 caacagatcg caccccacct ccgcccaaac cagacattgg gctttattca tggttacaac
     2281 atccattacc ggcacattac gcccccacca aacctcgacg tcgtcctcgt cgcgccaaag
     2341 gcacaaggcc gagcggttcg aagtgagttc ctggccggcc ggggtgtcgc cgcgctcgtg
     2401 gccgttcacc aggacgcgac cggtcgcgcc caacaaacgg ccctcggctg ggccgcggga
     2461 ctgggctgcc accggaccgc aatcttaaag accacgtttg ccgacgagac ggagactgac
     2521 ctcttcggcg agcaagccgt tctgtgcggt ggcttggtgg gtctgataaa ggccggcttc
     2581 gatacgttgg ttgaggctgg ttatccgccc gagttggcgt acttcgaatg ctgccatgag
     2641 gtcaagctga tcgcagacct gatttacgag catgggatca ctggaatgcg cgagcgaatt
     2701 tcaaacaccg cgcgtttcgg cgacctgacc cgcggcccgc gcgtgatcgg cgccgccaca
     2761 cgccaagcca tgcgcgaggt gctcgccgaa gtgcgctccg gccaattcgc ccgcgagtgg
     2821 gcggatcaga cacagcaggg gcaaccggag ttccggcggc tgacggacgc cgatcgcgga
     2881 cacctcatcg agcaagtcgg cgccacacta cgcaagctca tgtggggtca atcgccgcag
     2941 gcgccccact agcgtcccgg tggtcagcaa caaacatccc gcccaggccg cactgaccgg
     3001 tctggtcgcg cagacaaatc acttttgcct ggaacggcgc cagtgttata ctggacttgt
     3061 agtattcgcc gtcggcgcgc gccgctgcta gccaactgat tttgccgctg tcgggaagga
     3121 gctgtcaggc atgctgagga tcatttctct cgtcgtcctt ttcacggccg ctgttgcgcc
     3181 ggccgacacc atatacgtca acgccaccct cggcagcgac gcctgggacg gacgttgcga
     3241 gtactgggac gggcagagct gtggtccaaa ggccacgatt caggccggaa tcgacgcggc
     3301 cgcggagcac gacgaggtgc tcgtggccga aggaatctac agtggccccg gcaaccgcga
     3361 cctcgacctg tacggcaaag ccctcacgtt gcgcgcggcg ggcggcgaac cgggcgattg
     3421 cgtgatcgac tgtgagcatt ccgggcgggc gttctacttc cacagcggcg aaggcgcgga
     3481 ttcgatcgtt gccgggttct tgatccgcca cgccgacggc gacgccggca ccatttactg
     3541 ctacggcggg gccagcccta ccctggtcga ctgtacgatt acaaacaata cggccaataa
     3601 tggagggggc ctcttctgcg acgaaggcag ccgcccgacg ctcacccggt gcgtaatcgc
     3661 caacaacctg gccaactgcg gcggcggcgt tgcgtgcaaa tcctattcag acgccacgct
     3721 ggtggactgc acggtcgcgg acaatctcgg cgcttatggc gccggcgtgt ggtgttcgga
     3781 ccattcgaat gtgacgctcg ttgattgcat tatcagcggc aatgcgaacg gcgattttgg
     3841 cgcgggtgtg gactgctacg attccgatcc gaccctcatc aactgcgtca tttcgggcaa
     3901 ttccactacg tatgccggcg gcggcctgca cttctaccat tccaacgggc tgctggtgaa
     3961 ctgctcgatt tccaacaatg acggcggcga cagcgccggc ggtatttaca gccgctacga
     4021 ctccgccccc accctgatcg actgcctcat agccggcaat acgtccagta actgcggcgg
     4081 gggcgtatat tgccactacg cgacggccta catgatcaac tgtacgctta gcggcaacca
     4141 agcccgtttc ggcgggggcc tggattgcct accggactcc agcccggtgc tgacgaattg
     4201 ctacatattc ggcaacaccg caaccgcgcg gggcggcggc atgcgctgct atgaatccga
     4261 cccgatcctc aacaattgca tcatcgctca taattcggcc tcggagtccg gtggcggtct
     4321 gtactgcgag taccaatcta acgcggtgtt gaacaactgc accatcaccg gcaacacggc
     4381 cggaaccggg ggcggtcttg cctgcaacca ggaatccgcc ccaagcctga ccaactgcat
     4441 tctttggggc gacacgccca gcgagatttt cttggacggg ggcgcgccaa tggtcacctt
     4501 ctgcgatgtc cagggcggct ggagcggaac gggtaatatc cacgccgatc cgctttttcg
     4561 ggacgccgac gggcccgacg gcgacccaca ctcgtggcac gacaacgatt accggctggc
     4621 gagcgggtcg ccctgtatcg acgccggtga tcccggcttc gcgcccgagg cgggcgcgac
     4681 cgatgcagac gagcgcacgc gcatctggga cggcgacggc aacggccagc cgcgcgtgga
     4741 catgggcgcc tatgaattcg gctcgttccg cttcggcgac gtgaactgcg acggctcgat
     4801 caacgtgttc gatatcgatc cattcgtgct ggctttggtc gatcccgcgg cgtacgccga
     4861 ggaatattcc ggttgcgacg cttcgctggc tgaccttgac cacgacgggc acatcaactg
     4921 cttcgatatt gacccgttcg tgggcgtact gacggcgggt gattccggcg acttatgatt
     4981 tcggcctgaa cgcgcccacc agaaaagctg ggctcttgcc attgggccgc acgcggaagt
     5041 ctatgacgct cttcggctgg taaagaacgc ctcccagcca caccaaggcg cgttccattt
     5101 gccggaagat caggagcgat ttggggtcct gcttcattga gaccaccagc acgccaccgg
     5161 gggatagggc cgctgaatag cgcagcgttt gctccaatgc aacttgcggg gtgaggtagt
     5221 aaacgacttc gctcaggaca atcgcgtcaa actcgctacc cgcgtcaaac tggaaatctg
     5281 cgatgctgct gtgctggaac tcggtgcggc cggggcgggc cgcgcgctgc ctgcggcgct
     5341 gcagcgcatc ggcgatcgcg accgcgctga tgtcaatgcc caggtagtca acgtccgccg
     5401 gcagatcgta ggccagcgta ccgccggcgc atcccacatc gagcacgcgc cgcaacggcg
     5461 ggccgtaggc gcgcaagacg ccaacaacta tagcattgct ctgaatcacg gcccgcgtat
     5521 cgccgaggta atcgaccatg tgtccggaca gggctgcgtc ccagtagctt tgctgttcgt
     5581 gctttcccgc cggctgcagc acacgcgtca aaaaggatag gcgcgggttg tagtagaaat
     5641 gccgcaacca ccggcgcgcc gggcgtccaa gacgttgctg tagagtcgtc ataagaaaca
     5701 ctaggcttct cgcgccggga ccggctttta gaccgcgccg ccctgacagg ctaatgactt
     5761 caagctgaat gccgcgaaaa tctgcgtggc gtcctatgca cggcccgtca gcctcacggg
     5821 gtgtaagcat aaactccagc gcaacaggaa caagcggccc ctcgattttt ttcggggcaa
     5881 gctagcgccc gcaatcttcc gtggatgccc ggcgcgggac cgttcgcccg ggctcgcccc
     5941 agggggcttt gcagggccaa gcgcctctcg ctagactgcg cctcggatcg cggcgccagc
     6001 ggcgccgcgg cggcgcgatc cgcctggcgg cgggactccg acggcgcgcc ccggcttgcg
     6061 aaggaggact tggtaatgca tcgctacctg caaacggccg cgtgcggctt atgtatcctg
     6121 ctggcgggcg ccgctttcgc ccagcaagtg ggcgaagagg ccccggacct gaaacttccc
     6181 aaaagcgagc acgccacatc ggagaaagac ctcatttcgt tattcgaact ttacccgcac
     6241 cgcatcctcg tgctgtacta ttggagcagc ctggacagcg cgtcgctgga tttcttctcg
     6301 acgctcgacg agctggagga aaagtacggc ccgcggggcg taacgatcct cggccttacc
     6361 cgccagcaca agaaagtcat ggagaaggtg gctaaggagc gaaagccgaa gttcggcctt
     6421 ctgcagctta gtccgacgat ggggactttt cgggactacc ggcgaatctg gcgctttcct
     6481 gccccaggag tagcctacat catcgacacg caccggcgtg tggcctatgc ccggttcgac
     6541 gcaacggatc cgcgagagaa actggaagag aagattcaga cggtgcttgc gaagacgccg
     6601 acgctggccg ccaccgagga gctactgcag aaaaacatcc agaaagtcag caatttcatt
     6661 gaacagggcg agttcggccg cgcttacacg ctgggcacgg ccctgaagga attcttccgc
     6721 gaccgggagg acagcgcgtt ggaagagcaa gctgaagagc tgctgcggca gatcagcttg
     6781 ggggcccaca gacagctcga atccgcccag caggacttca aggaggaaaa atacgaggac
     6841 gccgctgcca agttggccga gttgagcgtc cgtctgaccg ggctgggcga cggcggcgac
     6901 aaacgcggtc gccggcgctc ctcgcgctcc acccgctcgg gcgcccggcg atccccccgc
     6961 ggcggccagg gggtagaacc gggcaaggat ttccgtgaaa tcaaggaaaa cgtcgagcgc
     7021 gaaatcgggc gcatgcaaag cgaaaactac accaaggcca tcattctcaa ggcgctcgat
     7081 aacgcgcgcg gccaggtgcg caacgacagt gcggcagagt gcatggaatt cgggcagtat
     7141 tctattgccc ggcggctcta caactcagtg gtcgaggagt tctccgacac cgaggccgcc
     7201 gaggcggctc aggaagccct ggaccgcatc gagaacgacc cgcgaataca ggaggcgatc
     7261 gccaaatcgg aggccagcga acaggccgag cgctggtacg atctgggaga tcggtttgcc
     7321 cgggccgaga tgttcgacaa gtcgcgcgag tactttgaga aggtcatcaa aggctatccg
     7381 gatagcgcgg cggcgcagag tgcccgcgac cggctggcgc gtatggaaga tgatgagaag
     7441 gcggccggcg aagggcgcca gaagcgcgag aagctggtgc ctccgaagga tgacaccaag
     7501 atcgttgagc aggcccgcac tatcttgcag aagctgatcg acggcgacta ccggggtttc
     7561 gtcgaagccg gcgacgatca gttgcgtgcg agtttgagcg agcaaaaggc tcgccaattg
     7621 tgggccgacc tgcgaaccaa actgggcgag ttcaaatcgg tcgaccaggg caagcttact
     7681 tccatactcg ggctccgagc cgtcacgttc gtggcgcaat tcgagcgcgg cgaacggaga
     7741 ctgcagatcg tgttcagtac gcagggcaaa atgaccgagt tcaagttgct agtagagccc
     7801 taaacgcggg catgccggac agggttgaac gaatttaaat tcaggctcgg ctggcgttca
     7861 cgccgtcagt cggcgccccc gcggatcaag cggctgaacg ccgcgccgcg cgcccggtag
     7921 tctttgaact gatcccagga ggcgcaggcc ggcgacagca gcaccgtgtc accggcggcc
     7981 gcttgcgccc gacaggcgct taccgcgctc gccaggtcgc cgcagtattc ggcctgcccc
     8041 cctgccgtcc ggatcagctc cgcaatctgc ttgccagtct gaccgatgca ggcggcgtac
     8101 ttcaaacgaa ttgcggccgc ccgcgcaaac tccccaaagt cgctgccctt gtcgtaaccg
     8161 ccaaggatca tcaacagcgg cccatcaatg gcttgcatcg ccgtggccgc cgcggtcggc
     8221 gtggtcgact ttgagtcgtt gtagtacgtg acgccgtcga tctcccccac gcgttgcagc
     8281 cggtccggca aggcttcaaa cgtggccaag gccgcgcggg agcgttgcgg ctcgattccc
     8341 agcaggtgcg ccaccgtcaa ggcggccgcc gcgttgagcc ggttgtgccg gcccggcaca
     8401 gccagccgcg tgtccggcca gcgcaagcgg cgatcgtcgc aatcaacggc ggcggtggac
     8461 tggcagaccg cgatggggtc gtcgccgtgc agtccatagc gccacacgcc ggacagatcg
     8521 ccaaatagcc gatcgaaatg ccgctgcaat tcgggatcat cgccgataac gaccgcgtcc
     8581 cgcgccggat cctgaaagcg cacgatattg agcttcgcgg ccaggtagcc ggcgaagttg
     8641 ccgtgccagt ccaaatgatt gggcgagatg ttcgtcagaa cggcgatatg cgggctccaa
     8701 gccaccagcg gcgtgcgctg caactgaaag ctggagagct cgaggacgac tatgtcctca
     8761 gtccgaatct cctccagcac ctccagcagc gagcgcccga tgttgccccc cacccacacg
     8821 cgacggccgc caccagaagc atcaaaagcg gagcaagacc cctcactgtc gatccctaga
     8881 tcccttgatc ccctgatccc ttgatccctc gatccctcga tcccttccct cagcacatgc
     8941 cccaccatgg ccgtgacggt gctcttgcca accgacccgg ttactccgac gcagcgcgcc
     9001 cggcagcgtt ccacgaacag attaatctcg gtcgtcagtg gcacgcccgc ggacacagcg
     9061 gcctgcagga acggggaggt atccggcacg gcgggattga ttacaaccag gtcggtttcg
     9121 cggaaatcgc gttcgtcatg gccgcccaac cggtaattaa tctcaagccc gtcgagctgc
     9181 gccagtgaat cgcccagctc cgccgccggg agcttgtcgg tcagggtcag acgtgccccg
     9241 gcgcgggcca gccagcgtgt gact
//
LOCUS       DAOSYS010000144         9189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6413_length_9189_cov_5.774031, whole genome shotgun sequence.
ACCESSION   DAOSYS010000144 DAOSYS010000000
VERSION     DAOSYS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6413_length_9189_cov_5.774031"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="PLU99_09835"
     CDS             complement(<1..1958)
                     /locus_tag="PLU99_09835"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOO17397.1"
                     /translation="MTLRTKLLTSKLCLAIIPVTFVGAVSFWQVTRAFDHFATQTNAA
                     LRDNTDAARTALIKSQEIDLEHVAQQIWNACLVQQSFLEQKLGADLNIARDLLRRAGA
                     VSLAEDRIEWQATNQFTKATQTVSLPKMVLGGKPIERITEPTRRAPLVDDVSDLVGST
                     CTIFQRMNPAGDMLRVCTNVQTLEGSRAVGTFIPAIGPDGKPNPVVAKLLKNEPYRGR
                     AYVVNAWYITAYDPIVNEAGEVTGALYVGIKEQSMDGLRNAIMATKVGKTGYVYVMNA
                     TGDHRGHYVISERGERDGEDIWNSRDANGDLFIQNVCNMAVNLKPGEVGHSRYPWKNA
                     EDTVARDKVVKVSYFAPWDWVISVGAYEDEFYEAVNAMEQRTQEAEVATAQTRQAALG
                     SVIGWTAGIGGITLFATIIVALIIIRSITHPLNRAIAGLNEGSEQVSEAAAQVAAASQ
                     HLASGASEQASSLEETSSALEQMAAMTRQSAENARQASVLAGEARASAQTGDSTTQRL
                     TQAMSAINESAGQINKIVKVIEEIAFQTNLLALNAAVEAARAGEHGKGFAVVAEEVRN
                     LAQRSAQAARETTGLIEDSVKRAREGSQVTMEVGESLSSIVQSVAKVTDLVGGIATGS
                     DEQAQGVEQINSAVSQMDKITQMNAASA"
     gene            2184..2257
                     /locus_tag="PLU99_09840"
     tRNA            2184..2257
                     /locus_tag="PLU99_09840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2218..2220,aa:Leu,seq:taa)
     gene            2718..7184
                     /locus_tag="PLU99_09845"
     CDS             2718..7184
                     /locus_tag="PLU99_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17398.1"
                     /translation="MKAWNVLRVGALAAVAVGSVAWAGVEVRLSGSSYQLALDGGTAF
                     HETANHVSGARTIPVPDSASVLVLWNEQTALGNVPFYAISLDGRQVDCVREANYDIML
                     RAATFDPGKSVPAVAADLGTGGNVFIVQFVTQILPEFSVQIEQLGGRVYHFLPNYSYV
                     VRMDAATRAAVAALPYVRWIGLYQPGYRIEEVLRDNRANLEEHFPWQRYIIMVFEPGL
                     KQKHAVGQRIKALGGIVNLPDAGKYTLEATLTPAQLWQVITWDEVHFVDRWSPFEKDM
                     NNARQYGGADYVETVAGYDGAGVRGEVFDAGFNMTHGDFASRPLIAHGSVGSDSHGAA
                     TSGIVFGDGAGNPLARGMLPAGQGIVGDYNTIGLTGPNRYQHIAEQVQAPYYCVFETA
                     SVGSDRVTTYTSISAETDAALFDFDILHCQSQSNAGTQMSRPQAWAKNILSGGGVYHY
                     DTLGTTDDCWCSGASIGPAADNRIKPDLCAYYDDILTTTTGGTTAYTTGFGGTSGATP
                     IIAGHCGLFFQMWADGIFGNEVDPAGTVFDNRPHMTTAKAVMINTARQYPFSGTGHDL
                     TRVHQGWGFPSAQNIYDLRERMFIVNEEVVLGALDSMVYTLAVDSGTPEFKATLVYAD
                     PPGSPSASLARINDLTLKVTAPDGTIYWGNNGLLGGNYSTAGGSANTVDTVENVFVLN
                     PAGGIWTVEVIASEINGDGHVETPELDADYALVVSGVLPCSSAGNIRFNRTIYSCQVA
                     AELRVLDCDLNTDPLAVETITVTVASDLEPAGEPVVLTETGAATAAFIGLIPLSDVDA
                     PFVCGSWFAPLNRKFFDVIVSTATYIDADDGQGHYDVVVTDSATIDCLSPEIFDIGAH
                     DIQPRSANISFEANELVSGTVFYGYSPTALTQQAGNPALVFTPVIALTALQDNRTYYY
                     AVQATDAAGNWVYDDNGGACYTFTTPEVPDPFTEHFDTSPFDLQYRSVTFEPNGSTDF
                     YFGCVGPIDVLPTDPTGGEELQLGDDTFVRVAPGAQVSLYGVSYNDFYVGSNGYITFG
                     AGDSASSPSIATHFNRPRISGLFDNLNPSGEAASVSWKLAGDRVAVTWLNVPESGTAS
                     QNTFQIELFFDGRIRISYLDVSAVGSLAGLSRGAGVPADYFESDLTTLGPCFPIQISL
                     PSGAPTLLAPGQPTLVPVRIEDGRQFYVPDSALLHYRSQSGPFESIPLTHVSGEMFEV
                     TLPAFACGALPEFYFSAAGDGGANVTLPEGGPDGPFQAGVGILTVLLEDNCEEDQGWT
                     VVNDPSLQEGEWERALPRADDRPQAPKADFDGSGKCFLTEAFWSRDVDLGPTMLLSPV
                     FDLADGGAVFLRYARWIACDDVGSPAQDFLDVALSNDGGATWVLVEHVTSGGGWVQRE
                     FRVNDYISLTSEVQVRFSVMDTPVNSITEAAVDAVRLYDISCDLTAETGDLNCDGAVN
                     AFDIDAFVLAITDPAGYAAAYPACSYLLADCNGDGSVNAFDIDPFVALLTQS"
     gene            complement(7412..7654)
                     /locus_tag="PLU99_09850"
     CDS             complement(7412..7654)
                     /locus_tag="PLU99_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17399.1"
                     /translation="MFGMPAHLGRHMNAVHGIGKPARAGRKPARGRGRPAGPTTPDGF
                     GQAESHLQRARESLLQQRATIDSQIEKIGQVLEALS"
     gene            8893..>9189
                     /locus_tag="PLU99_09855"
     CDS             8893..>9189
                     /locus_tag="PLU99_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17400.1"
                     /translation="MPARNAGSPINAGQSAAAPGSATRFVIRPAAAGGRLHEFDTSVG
                     TAAPPDTLGGYLMTPFGGDPSPLATDVTSVTGPCPISGSIQFSIPCNHRRIGSGW"
BASE COUNT         1606 a         2928 c         2900 g         1755 t
ORIGIN      
        1 tcggccgagg cggcgttcat ttgcgtgatc ttgtccatct gcgagacggc gctgttgatc
       61 tgctcgacgc cctgagcctg ttcgtccgag ccagtggcga tgccaccgac caggtcggtc
      121 accttggcga ccgactggac gatcgagctg agcgattcac cgacttccat cgtgacttgc
      181 gatccctcgc gggcgcgttt gacggagtct tcgatcaaac cggtggtttc gcgggccgct
      241 tgcgcggagc gctgcgccaa gttacggacc tcctcggcca cgaccgcgaa gcctttgccg
      301 tgctcgccgg cccttgccgc ctcgacggcc gcgttcagcg ccagcaggtt agtctggaag
      361 gcgatctcct cgatgacctt gacgatcttg ttgatctggc cggcggactc gttgatggcc
      421 gacatggcct gtgtcaggcg ttgcgtggtg ctgtcgccgg tctgggccga ggcgcgggcc
      481 tcaccggcca gcacgctggc ctggcgagcg ttctcggcgc tttgccgcgt catggcggcc
      541 atctgttcaa gagcgctgga ggtttcctca agcgaggagg cctgctcgct ggcgccgctg
      601 gccagatgct gcgaagcggc ggcgacctgg gcggcggctt cggagacctg ttcggagccc
      661 tcgttcaaac cggcgatagc gcgattgagc gggtgcgtga tgctgcggat gatgatgagc
      721 gccacgatga tggtcgcgaa gagcgtgatg ccgccaatgc cggcggtcca gccgatcacg
      781 gagccgagcg cggcctggcg tgtctgtgct gtagcgacct cggcttcctg ggtgcgctgc
      841 tccattgcat tgacggcttc atagaactcg tcttcgtagg cgccaacgct gatcacccag
      901 tcccagggag cgaagtaaga caccttgacg accttgtcgc gcgccacggt gtcttcggcg
      961 ttcttccagg gatagcggga gtggccgacc tcgccgggct tgaggttcac agccatgttg
     1021 cagacgttct ggatgaaaag gtcgccgttg gcgtccctgg aattccagat gtcctcgccg
     1081 tctcgttcgc ccctctccga gattacgtag tggccgcgat gatcgccggt cgcattcata
     1141 acgtagacgt agccggtctt tccgactttg gtggccatga tggcgttgcg cagcccgtcc
     1201 atactctgtt ctttgatgcc tacatacagc gcgccggtca cttcgcccgc ctcgttcaca
     1261 atcgggtcgt aggcggtgat gtaccaggcg ttgacgacgt aggcccggcc gcgatagggc
     1321 tcgttcttga gcaacttcgc gactaccggg ttaggtttgc cgtccgggcc gatggccggg
     1381 ataaaggttc cgacggcgcg gctgccctcc agagtctgca cgttggtgca cacccgcaac
     1441 atgtcgccgg cggggttcat gcgttggaag atcgtgcacg tgctgccgac aaggtccgaa
     1501 acgtcgtcca ccagcggagc cctcctggtg ggctcggtga tccgctcgat cggtttgccg
     1561 cccaggacca tcttcggcag cgacacagtc tgggtggcct tggtaaactg attcgtggcc
     1621 tgccattcga tccggtcctc ggccaaggag accgcgccgg cgcggcgcag gaggtcacgg
     1681 gcgatattca agtcggcccc caacttctgc tccaaaaagc tctgctgcac caggcacgcg
     1741 ttccaaatct gttgggccac gtgctccagg tcgatttcct gggatttgat tagcgcggtc
     1801 cgggccgcgt ccgtattatc gcgcagggcg gcgttggtct gcgtggcgaa gtggtcgaag
     1861 gcgcgcgtga cctgccaaaa cgaaaccgcc cccacgaatg tcaccggaat gatcgccaag
     1921 cacaatttgg acgtgagcag tttggttcgt aaagtcatgt tccttcctcg taagaaacgg
     1981 tcacctcgcg caggtctccc gacggctctg gcgacggtgt ttacatccag cgcgcggcct
     2041 cgcggcagcg gcacgctctc agttccgccg tattgatcgt cccggatcgc gcggttgtat
     2101 aattgcggga gccgatttcc cgcgctaaaa gcgtttatga gagcgcggca ggccgggacc
     2161 aggtccggta caatgaggcc caggcgccag tagcccaata ggcagaggca ggggatttaa
     2221 aatccccaca gtacgggttc gaatcccgtc tggcgcagtg ttgagggaac gtcgccggtt
     2281 cagcaactcg cccgcacgtt ataagtgccg gcgagctcgt aaacccagca gcgccaaata
     2341 tttacgtcca ccgccatcac cccaggaact gaacccctca tgcgctggaa cttggccggc
     2401 ccggcagccc gcaagtaccc gcgcaacgga aagcggacaa cccgaggcga caggttttgc
     2461 agcacacgtc gcgatcacgc ggatttggct tggccggaag attgcgtttc accagcgcca
     2521 cccactggtt aagatggacc gaggtgactg gctggtgaca agcgcggacc ggcggccgtg
     2581 tggaagggaa aggagtggcc tttctgccac gcccggcgcg gctctgcgca ctggcaacac
     2641 ctttttttcg ccgcgcacat tcgagtttga caaaacgcgt tgcgcgaagg cgtgcccgaa
     2701 agggcgaagg agcacaaatg aaggcctgga atgttctccg tgttggtgcc ctggcggccg
     2761 tagccgtggg ctcggtcgcc tgggccggcg ttgaagtacg gttgagcggg tccagctatc
     2821 agttggcatt ggacggaggt acggcctttc acgagactgc caaccacgtc agcggtgcgc
     2881 gcaccatccc cgtgccggat tccgcgagcg tcctggtcct gtggaacgag cagaccgcgc
     2941 tcggcaacgt tccgttctac gccatcagcc tcgacgggcg acaggtagat tgcgtgcgcg
     3001 aggcaaacta cgacatcatg ctgcgggcgg cgacgttcga ccccggcaag tccgtgccgg
     3061 cggtggccgc ggacttgggc accggcggaa acgtcttcat cgttcagttt gtaacgcaga
     3121 tactgcctga gttcagcgtg cagatcgaac aactcggcgg ccgcgtctac cacttcctgc
     3181 cgaactattc ctacgttgtg cgcatggacg ccgcgacgcg cgccgcagtg gcggcgctgc
     3241 cgtacgtgcg ctggatcggc ctctatcagc cgggctaccg gatcgaagag gtgctgcgcg
     3301 acaaccgcgc caacctggag gagcacttcc cctggcaacg ctacattatc atggttttcg
     3361 agcccggcct gaagcaaaaa cacgccgtag ggcagcgcat taaggccctc ggcggcatcg
     3421 tgaacctgcc ggatgccggc aagtacacgc tggaagccac gctcacccct gcgcagctct
     3481 ggcaggtgat cacctgggat gaagtgcact tcgtcgaccg ctggagcccg ttcgaaaaag
     3541 atatgaacaa tgcccgccaa tacggcggcg cggattatgt cgaaaccgtg gcgggctacg
     3601 acggcgccgg cgtgcgcggc gaggtcttcg acgcgggctt caatatgacg cacggcgact
     3661 tcgcctcgcg cccgctgatt gcgcacggca gcgtgggcag tgactcacac ggggcggcga
     3721 cctcgggcat cgttttcggt gacggcgccg gcaatccgct ggcgcgcggc atgctgccgg
     3781 ccgggcaagg catcgtgggt gactacaaca ccatcggcct gaccggtccg aaccgctacc
     3841 aacacattgc cgaacaggtg caggccccct actactgcgt cttcgagacg gccagcgtgg
     3901 gcagcgaccg cgtgacgacg tacacgtcga tttcggccga aaccgacgca gccctgttcg
     3961 atttcgacat tctgcactgc cagtcgcaga gcaacgccgg cacgcagatg tcacggccgc
     4021 aggcctgggc gaagaacatc ctctccggcg gcggcgtcta tcattacgac acgctcggta
     4081 caactgatga ttgttggtgc tcgggcgcca gcatcggccc ggcggctgat aatcgcatca
     4141 agcccgacct gtgcgcctat tacgatgaca ttctgacgac caccacgggc ggcactacgg
     4201 cctacacgac cggcttcggc ggcaccagcg gcgcgacgcc gatcatcgcc gggcattgcg
     4261 gcctgttctt ccagatgtgg gccgacggca tattcggcaa tgaagtcgat ccggccggca
     4321 ctgtgtttga caaccgtccg cacatgacga ccgccaaggc ggtaatgatc aacaccgccc
     4381 ggcagtaccc gttcagcggc accggccacg acctgacgcg cgtacaccag ggctggggat
     4441 ttccgagcgc gcagaacatc tacgacctgc gcgagcgaat gttcatcgtt aacgaagaag
     4501 tcgtgctggg cgccctcgac agcatggtct acacgctggc ggtcgattcc ggcacgccgg
     4561 aattcaaggc tacgctggtt tacgccgacc cgcccggctc gccctcggcc tcactggcgc
     4621 ggatcaacga cctaaccctc aaggttacgg ccccggacgg cacgatttac tggggcaaca
     4681 acggcttgct gggcggcaat tactccaccg ccggcggttc ggccaacacg gtcgacacgg
     4741 tcgagaacgt tttcgtccta aacccggcgg gcggcatctg gactgtcgaa gtgatcgcgt
     4801 ctgagatcaa cggcgatggc cacgtggaga cgcctgagct ggacgctgac tacgccctgg
     4861 ttgtcagcgg cgtgctgccg tgcagttcgg ccggcaacat ccggttcaac cggaccattt
     4921 actcgtgcca agtcgcggct gaattgcgag ttctggactg cgacctaaac acggatccgc
     4981 tggcggtcga gacgataacc gtgaccgtcg cgtccgacct cgagccggcc ggcgaaccgg
     5041 tcgtgctgac ggaaaccggc gcggccacgg cggccttcat tgggcttatt ccgttgagcg
     5101 acgtcgatgc gccttttgtg tgtggcagtt ggtttgcacc gctaaatcga aagttttttg
     5161 atgtgattgt ttcgactgcg acgtacatcg atgccgatga cggtcagggc cactacgacg
     5221 tggtggtcac ggacagcgcc acgatcgact gcctgtcgcc cgaaatcttc gatatcggcg
     5281 cgcacgacat ccagccgcgt tccgccaaca tcagcttcga ggccaacgaa ttggtgagcg
     5341 gcacggtttt ctacggctac agcccgacgg cgctgacgca gcaggccggc aatccggcgc
     5401 tggtgttcac gccggtgatc gccctgaccg ccctgcagga caaccggaca tactattatg
     5461 ccgttcaggc gacggacgcg gcgggcaatt gggtctatga cgacaacggc ggcgcgtgct
     5521 atacgtttac cacgccggag gtgccggacc cgttcaccga gcactttgat acctccccct
     5581 ttgacttgca atatcgcagc gtgacattcg agccaaacgg ttcgaccgat ttctacttcg
     5641 gttgcgtggg cccgatcgac gttctgccga ccgatcccac gggcggagag gaactccaac
     5701 ttggagatga cacgtttgtt cgggtcgccc ccggagcgca ggtaagccta tacggcgtgt
     5761 cgtacaacga cttctacgtc ggcagtaacg ggtacatcac gtttggcgcc ggcgactccg
     5821 cttccagccc gtccatcgcg acgcacttca accggccgcg catctcgggt ctgttcgaca
     5881 atctcaatcc ctcgggtgag gccgccagcg ttagctggaa gctggccggc gaccgcgtgg
     5941 cggtcacctg gctgaatgtc cccgagtccg gcacggccag tcagaacacc ttccaaatcg
     6001 agctgttctt cgacggacgc atccggatca gctacctcga cgtgagcgcg gtcggttctt
     6061 tggccggcct gtcccgcggc gccggcgttc cggcggacta cttcgaatcg gacttgacga
     6121 ccttggggcc ttgcttccct atccagatct cgctacccag cggtgcgccg acgctgcttg
     6181 cgccggggca gccgacattg gtgccggtgc gaatagagga cggccggcag ttctacgtgc
     6241 ccgattcggc attactgcat taccgcagcc agagcgggcc gttcgagtcc attccgctca
     6301 cgcacgtcag cggcgaaatg ttcgaagtca cgctgccggc atttgcctgt ggcgctttgc
     6361 ctgagttcta tttcagcgcc gccggcgacg ggggcgccaa tgtgacgttg cccgagggcg
     6421 gcccggacgg tcccttccaa gccggcgtgg gaatcctgac ggtcttgctc gaggacaact
     6481 gcgaagaaga ccagggctgg acggtggtca acgatcccag cctgcaggag ggggagtggg
     6541 agcgcgcact gccgcgcgcc gatgaccggc cgcaggcgcc taaggccgat ttcgacggct
     6601 ccggcaagtg cttcctgacc gaggctttct ggtcgcgtga cgtggatttg gggcccacta
     6661 tgctgctctc acccgtattt gacctcgcgg acggcggagc ggtattcctg cgatatgcgc
     6721 gctggatcgc gtgcgatgac gtcggctccc cagcacagga tttccttgac gtcgcattga
     6781 gcaacgatgg cggggccacc tgggttctcg tcgaacacgt gaccagcggc ggcggatggg
     6841 tacagcgcga attccgcgtc aatgattaca tcagcctgac gtcggaggtc caggtccgct
     6901 tctcggtcat ggacactccg gtcaattcga tcaccgaagc cgccgtcgat gccgtacgac
     6961 tgtacgatat ctcgtgtgat ttgaccgcgg agacgggcga cctgaattgt gatggagcgg
     7021 tcaacgcctt tgacatcgac gccttcgtgc tggccatcac cgacccggcc ggctacgccg
     7081 cagcctatcc ggcgtgcagc tatctcctgg ccgactgcaa cggcgacgga agcgtgaacg
     7141 cgtttgatat tgacccgttc gtggcgctgc tgacgcagag ttgagatggg ccgccaaatg
     7201 gccccgcctg aatgacgcgg ggccattcgg taccaggagg cccctcggcg ccgccaggaa
     7261 ttggcgcaaa gtatgcggac cggccgcctg atctggcggc cggcttcgca tttgcgtcat
     7321 ctggtctggg ttgggcgatg caggcccctt ggccgctggg atgcacctac agcggaccgc
     7381 gcaccgccgg cagacgtgcc cgttaacggt atcaggaaag ggcttccagc acttgcccga
     7441 tcttctcgat ttgcgagtcg atcgtggcgc gctgctgcag cagcgattcc cgggcgcgtt
     7501 gcaggtgcga ctcagcctgc ccaaagccgt ctggggtcgt tgggcctgcc ggccgcccgc
     7561 ggccgcgggc cggcttgcgt ccggcacggg ccggcttgcc gattccatgc acggcgttca
     7621 tgtgccgccc caggtgagcc ggcattccaa acatacgccc gcattgagca cactgcaatt
     7681 tcttcgttga tctaaccatt agttgttcct ctccccggta gttctctcgg ccacacacaa
     7741 tggcccgcta gtatactgtt ctcgccgccg agtaccagta ccccaagcaa gaattccacg
     7801 cagtttttca gaatcgtaag ccattgacgt tgctgtactt gcggctgtgt cggcggcccg
     7861 ccattagatt atagcgccgg aagccggcgg aagacgggat tcttgcggca taccggccac
     7921 cggggcggct ccaaaagcac gcgccattgt aaatggcgct gccgcctatg ctctcggccc
     7981 agtgaaacca gtacgacctt gcttcgcgca catctccggc ggcatgcccc gaatcgtagt
     8041 ggcacgcgga ggcccccgca cttccgcaga gctttttgcc gccgggcgtg cgtggcggca
     8101 acggcggcac agcataccgc tgtgcatttg cgccggctct gatgtagctg tcggtgccag
     8161 caggtaggca ctggccaagg agcggccccg acaagtgagg gggcagagca atgatctccg
     8221 atctcgggtc ctcttgcgtc tggcattggg ggcagccacg cagcacgctc gccctgccct
     8281 gcggcttgcc cgaagattga cctgtccttg gccccaaccg ccgactttct gcggccagac
     8341 aacgaagccg ctggcggcca ttttgcgacc attctacaca gaagtggcgg acattcagcc
     8401 cccggtttgg tgatcccgtg ccgggcgatc cggattcctg cctgtgcggc gacttctgca
     8461 tcaggcccgg aaactgtagc ggcgccacat aggcgtcaac gacctctggc ctgaacgggc
     8521 gaactgcggg tcaaaaatcc tttcctgaac gaccgccggc gcgttatact gactctgcca
     8581 tttttgtacc ggctctcggc cgggactcgg cgggagcggc gcgaatcacg tttctaaaga
     8641 gctctgagga ggagaacgat gttatggcgc gtatcgttgg ccttgggctt ggcttcagcg
     8701 gctctgctca gcagcgctgc tttgcagcca agtgacggaa attcggctct gacaaaagaa
     8761 aatccgcctt gccaggcgat agcccagtcg ccggcagccg taaacgcgcc ggcgaccagc
     8821 gcgcccggcg caaacactgc cccggcgccc caatcacccg cgggcggctc aaacgtcggg
     8881 gcgcgtgaag gcttgccagc ccggaacgcc ggcagcccca tcaacgccgg acagtcggca
     8941 gccgcccccg gcagcgcgac gcgtttcgtc attcgacccg ctgcggccgg cggacggctg
     9001 catgaattcg acaccagcgt cggaacggcg gccccgccgg acacgctggg cgggtatctc
     9061 atgacgcctt tcggcggtga tccttcgcct cttgctactg acgtgaccag cgtgacgggt
     9121 ccgtgcccga tttccggctc gatccagttc agtattccgt gcaaccatcg caggatcggc
     9181 agcggctgg
//
LOCUS       DAOSYS010000145         9180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6425_length_9180_cov_5.701479, whole genome shotgun sequence.
ACCESSION   DAOSYS010000145 DAOSYS010000000
VERSION     DAOSYS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6425_length_9180_cov_5.701479"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLU99_09860"
     CDS             <1..765
                     /locus_tag="PLU99_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17401.1"
                     /translation="SNDCNENAVPDECDLAPGGGSEDCNENLIPDECELAPPIFVESA
                     DNCADALTVCPGRSYWGTTVGATPDGQASCGSAADSPDVWYYYRPRGNGFLTLSLCGS
                     SFDTVLSVHSGCPGDVSNQIVCNNNSCGLQSEVSLYVQNQRPYWIRVSGEGTEAGDFQ
                     LTIVGPTCFYDDTDLDHDGILDECQIECGMMADANCDGVVNVFDIDPFVVALTSPAQW
                     SATYACDLLCANDCNGDGEVNVFDIDPFVLALIGGN"
     gene            1151..2620
                     /locus_tag="PLU99_09865"
     CDS             1151..2620
                     /locus_tag="PLU99_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOO17402.1"
                     /translation="MNICLACTALFFLVAAQQPENPWKARVPAAVATAQQENTTEAYC
                     RALDTAWRADDWQMGLALARESLEKWPESSELRGRQGRTFWRAGHIAPAEELAARISP
                     DTTDHVALRLLVQINAARGNLPAAATAAERLAELPPLTAEDLFAVLGARLAQNRLAGL
                     PEMIRRIEQLAAVANGYPENYMVEQLGGLAEFFEAVGSAPLNQIARFGEAVMPVMPLI
                     NLPGCQVMINGRGPYRMLVDTGGSITLSLDQAVAAELGLKSIADATIHGIGGREQSGQ
                     TLVEELTIGEIRCKRVMTRTFELRKSIAYAADGVLGTGIFADSRMTLDFEHGRLHVAA
                     ASDAPGPGREAQLRIVGDGKLLTPTLVNGTPLVGLLDSGADVFALSPALLEKLFPERP
                     IKSISAFTMGVGSDAQPSISMMPTVTLEVAGRKFADHGGVGLDVLDTLLSPMLGIQTD
                     ALVGMPVLRDMRTLTVDFAHSKMWVEWLAGSDGEKGLAE"
     gene            complement(2629..4656)
                     /locus_tag="PLU99_09870"
     CDS             complement(2629..4656)
                     /locus_tag="PLU99_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOO17403.1"
                     /translation="MRPRRTGELSGWFWRVAGAAVLFVAAGVDPAWGQVSRSELEELC
                     ARGEREGWTFSVGENPATARSLSELCGLVEPEDWRVGATFDPCRPRRALPAAFDWRTY
                     GGCTPVRDQRSCGSCWAFATVGPLECNILRLDGRSVDLSEQWLVSCNRSGWSCDGGWF
                     AHGYHLAAPDSCAGSGAVLESYFPYSARDLPCECPYPHEYHIAGWAYIESALENPAAI
                     KQAILDYGPVSVAVHVNSAFQSYVGGIFNDCQAGTINHAVVLVGWDDSDGVWIMRNSW
                     GPRWGENGYMRIVYGCSRIGYAACYVDYQMRDCNENSVPDLCDVRCGDGFCAGHACGE
                     SLDCNHNLIPDECEFDCNGNGVPDDCDIASGFSADCNENGTADDCEPDCNGNGVPDDC
                     DALSGTSPDCNRNLVPDECDLALGAADCNGNGIPDECDLYPPAFTAAQDNCSDAELLC
                     PDALQSGTTANTARDGRASCGGESEAGDVWYYYQPAGNGFVQISLCGSTFDTVLSVHS
                     GCPGDVSNQIVCNDNYCGLQSQVTLFVSAGHEYWIRVGGAAGAGGDFSLRLVGPACVY
                     SAECNDNGIPDECEPDCNGNGLPDDCDITAGRSTDLNGNGIPDECEALGDMNCDGVVN
                     SFDVDPFVLAFVNPQAYAEAFPGCAASNGDINADGALNVFDIDPFVLLLTK"
     gene            4844..5407
                     /locus_tag="PLU99_09875"
     CDS             4844..5407
                     /locus_tag="PLU99_09875"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOO17404.1"
                     /translation="MATSYCWGEVSDLMAAYHDNEWGVPVHDDQRLFEILLLDNAQAG
                     LSWETILQRRPNYRKAFAGFDPQRVARFTPRDVQRLLKDPGIIRNRLKIESAITNARC
                     FLEIQEEFGSFDAFIWQFVQGRPIRGRWKTWRDVPATTPESDAMSKELKRRGFKFVGS
                     TICYAIMQTAGLVNDHILGCPRRAVCG"
     gene            complement(5582..5965)
                     /locus_tag="PLU99_09880"
     CDS             complement(5582..5965)
                     /locus_tag="PLU99_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17405.1"
                     /translation="MPPAIQNAALSRHAGSIVDARQSGPDSWSLTVSTATGLRDLEVR
                     NDGLILSDIQSYKDDTVQNLPATVRSSVNSAFPGAMILNVDEGATDGQPAFRIEMLQN
                     GQQFFAIVAPNGQILNQGPLSPTGR"
     gene            complement(6783..8639)
                     /locus_tag="PLU99_09885"
     CDS             complement(6783..8639)
                     /locus_tag="PLU99_09885"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOO17406.1"
                     /translation="METAKLKAVAQAVPLEGAFAYPPFVQPVRTASARARFHGRDWVY
                     RNGFDAVLAKVPGPAWADPEGQGWQRVKRNARREVWRARLDGAAYYLKYYFGDGFAMR
                     VRRFFRGPACEAEWKGGIYALQAGIPAVAPAGYTTDLIKNGARCSLLVTEAVEPAYAL
                     DEFWEQVVADDDLARRRRDTAQLVKALAQMIARAHQAGFEHLDMHAANILVQPLAPRR
                     YRPLFVDLQSARRGVPLSDAAVVRNLAQLNQWFRRRSDVTARLRFLRAYLRYRNEYEH
                     AFEHGRPLGHSFRSLVQALARAAERHAELLGAQRDRRAMREGQYFTRLRAGEWRGMAV
                     IRTKHASADSPLSRLVLTPAWWREQLRDPLRWFRNGETESCKESHSAWVRRGMLSFEN
                     QPLPLIFKRPRARNWRRRLRQLLGVSRSVRGWRIGHALLNRHVAAARPVAVLERRWGP
                     LVLDSILITEAIPGACDLETWLRRTHAASRPDVWLMRKRQMADLMAQHLRRLHERGFE
                     HRDCKAGNVLVAGQPKSKLLWIDMDGLRRARRWSKKGELRALAALHVSLSDVQGLNLA
                     DRVRFLRAYTARFGADRRAWKTLWRQLEPLVAARTRARAARRLWKLQHYGRE"
     gene            complement(8857..>9180)
                     /locus_tag="PLU99_09890"
     CDS             complement(8857..>9180)
                     /locus_tag="PLU99_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17407.1"
                     /translation="VLNEMEHSRSSFRPGPGFISPPFEVLAESWVAREAAGALISEFS
                     TLLAGGTHSCLLRPLPPEDGYARVLVQRDYWPAGEILGFDESGELQFLRSAIGELRRV
                     TNRRS"
BASE COUNT         1713 a         3056 c         2782 g         1629 t
ORIGIN      
        1 agcaacgact gcaatgaaaa cgcggtgccg gacgaatgcg acctcgcccc cggcggcggc
       61 agtgaggact gtaatgagaa cctgatcccg gatgagtgcg agctcgcccc acccatcttc
      121 gtcgaatccg ccgacaattg cgcagacgcc ttgacggtct gtcccggccg tagctactgg
      181 gggaccacgg tcggcgccac gcctgacggc caagcgagct gcggcagcgc cgctgattcg
      241 cccgatgtgt ggtactacta caggcccagg ggcaacggtt tcctgacgtt gagcttgtgc
      301 ggctcgagct ttgacaccgt cctgtcggtg cacagcggct gcccgggtga tgtgagcaac
      361 caaatagtct gtaataacaa ctcctgcggc ctgcagtcgg aggtgtcgct ctacgtgcag
      421 aaccagcgtc cgtactggat tcgggtctcc ggcgagggga ctgaggcggg cgacttccag
      481 ttgacgatag tcgggccgac ctgcttctac gacgacaccg atctggacca cgacggcatt
      541 ctcgacgagt gccagatcga atgcgggatg atggccgatg caaactgcga cggcgtcgtg
      601 aacgtattcg acatcgatcc gttcgtcgtc gccttgacca gtccggcgca gtggtcggcc
      661 acctatgcct gcgaccttct gtgcgctaac gactgcaacg gcgatggtga agtaaatgtg
      721 tttgacatcg accccttcgt cctggcgctg atcggaggaa attagggccg ccattccaag
      781 attcgcggct caagcgggaa gtaaccagcg ccccgagccc cggtgggctt ggctaacgca
      841 ccctaccccc ggccggcgcc tgaacacgct ggcaaatcgg caccccgccc ggctgcttcc
      901 gtgcgcctcc gcgcgctgcg cgcggcacgt cggctgcaag actgtcccgc atcccaacgc
      961 gctgatggac cagcgcgtcg cctgacgaac ccccgcgcag cgatagcgtg ctgatgacaa
     1021 atcgtggccg gatcgaacct gcgaggcttg tcggaacccg ctcgtaccgg atatcgtgcg
     1081 acacggcgga tcgtgcgact agcgcgcggc ccccccttga acaccggcgc agttaacacc
     1141 ggagtagttg atgaatattt gcttggcatg cacggctctt ttctttctgg tcgccgcaca
     1201 acagcctgag aacccgtgga aggcccgggt ccccgccgcc gtcgcaacgg cgcagcagga
     1261 gaacacgact gaggcttatt gccgcgcgct tgatacggcc tggcgtgccg atgattggca
     1321 aatgggcctg gcactggcgc gcgagagcct ggagaagtgg cccgagtcca gcgagctgcg
     1381 cggacggcag ggccgcacct tctggcgcgc cgggcacatc gcaccggcgg aagagctcgc
     1441 cgcacgcatt tcgccggata cgaccgatca cgtcgccctg cgcctgctcg ttcaaattaa
     1501 cgcggcgcgc ggcaatctgc cggccgccgc gaccgcggcc gaacgtctgg ccgagctgcc
     1561 tccgctcacg gccgaggact tgtttgcggt cctgggcgcg cggctggcgc agaatcgcct
     1621 ggccgggctg ccggagatga tacggcgcat cgagcagttg gccgccgtgg ctaacggcta
     1681 tcccgagaac tacatggtcg agcaactggg cggcttggca gagtttttcg aagcggtcgg
     1741 gtccgcgccc ctgaaccaga tcgcgcgctt cggcgaagcg gttatgccgg tgatgccgct
     1801 catcaatctg ccggggtgcc aggtaatgat taatggccgt ggaccgtaca gaatgctggt
     1861 cgacacgggt gggagcatta cgctgtcact ggatcaggcg gtcgcggcgg aattaggtct
     1921 taagtccata gccgacgcca ccatccacgg catcggcggc cgggagcagt cggggcagac
     1981 gctggtcgag gaactgacga tcggcgagat aaggtgcaaa cgcgtcatga cgcgcacttt
     2041 cgaattgcgc aagtcaatcg cctacgcggc ggacggcgtg ctcgggacgg gcatatttgc
     2101 cgattctcga atgacgctcg atttcgaaca cggccggttg cacgttgcgg cggccagcga
     2161 cgcgcccggc ccggggcgtg aggcgcaact gcgcatcgta ggcgacggaa agctgctgac
     2221 gcctacactc gtcaacggta cgccgctggt gggcttgctg gatagcgggg cggacgtgtt
     2281 cgcgctatcg cccgcgctgc tggagaagct gtttccagaa cgcccgatta agagcatctc
     2341 cgccttcacc atgggggtcg gcagcgacgc gcagccgagt atcagcatga tgccgaccgt
     2401 gacgctcgaa gtcgccgggc ggaaattcgc cgaccacggc ggcgtggggc tggatgttct
     2461 ggacacgctc ttgagtccaa tgctggggat tcagaccgac gcgttggtgg ggatgcccgt
     2521 gctgcgcgac atgcgaacac tgaccgtcga ctttgcccac agcaagatgt gggtggagtg
     2581 gctcgcaggc agtgatggtg aaaaggggct ggccgaatag gccgcccact atttcgtcag
     2641 cagcaggacg aatgggtcga tgtcaaatac gttcaacgcg ccgtcggcgt tgatgtcgcc
     2701 attgctggcc gcgcagccgg ggaacgcctc agcgtaggcc tgcgggttta cgaacgccag
     2761 cacgaaggga tcaacgtcaa agctgtttac aactccgtca cagttcatgt cgcctagagc
     2821 ttcgcattcg tccggaatgc cgttcccgtt caaatccgtg ctccgtccgg ccgtgatgtc
     2881 gcagtcatcg ggcagcccgt tgccgttgca gtccggctca cactcgtccg ggatgccgtt
     2941 atcattgcac tcagcgctat agacacaggc cggtccaaca agcctcaggc taaaatcgcc
     3001 gccggccccg gcagcgccgc cgacccgaat ccaatactcg tgccccgcgc ttacgaacag
     3061 cgtcacctgg gattgcaggc cgcagtagtt gtcattacag actatttggt tgctcacatc
     3121 acccgggcag ccggagtgaa ccgaaagcac cgtatcgaag gtcgagccgc ataggcttat
     3181 ctggacgaat ccgtttccgg ccggctggta atagtaccag acgtcgcccg cttcggactc
     3241 cccgccgcaa ctggcgcgcc cgtcgcgcgc cgtgttggcc gtcgtgccgc tttgcagcgc
     3301 gtcggggcac aggagttcgg catcgctaca gttgtcctga gcggctgtga aggccggcgg
     3361 atacagatcg cattcgtcgg gaatgccgtt tccgttgcag tccgctgcgc ccaacgccag
     3421 gtcgcattcg tccggcacca ggttgcggtt acaatcaggg ctggttcccg atagcgcgtc
     3481 gcagtcgtcc ggtacgccgt tgccattgca atcgggctca cagtcgtccg cagtgccatt
     3541 ctcgttgcaa tcggcgctaa agccactggc tatgtcacag tcatccggaa cgccgttgcc
     3601 gttgcagtcg aactcgcatt cgtccggtat cagattgtgg ttgcagtcaa gtgattcgcc
     3661 gcacgcgtgc cccgcgcaaa aaccgtcgcc gcaccggaca tcgcacaggt cgggaaccga
     3721 attctcattg caatcacgca tctgatagtc gacatagcac gccgcgtacc cgatacgcga
     3781 gcagccgtaa acgatgcgca tgtagccatt ctctccccag cggggccccc acgagttccg
     3841 catgatccag acaccgtcac tgtcatccca gccgaccagc actaccgcgt gattaatcgt
     3901 accagcctgg caatcgttaa aaataccccc cacatagctt tgaaacgcgc tattcacgtg
     3961 gaccgccacc gataccggac cgtagtccag gatggcctgc ttgatggccg ccgggttctc
     4021 caaggcgctt tcgatgtagg cccagccggc tatgtggtat tcatgcggat atgggcattc
     4081 gcagggcagg tcccgcgcgc tatagggaaa gtagctttcc aggaccgccc cagagcccgc
     4141 gcacgagtca ggcgcggcga ggtggtagcc gtgggcaaac cagccgccat cacaactcca
     4201 acccgaccgg ttacaactga ccagccattg ctccgacaaa tccacgctgc gcccgtcgag
     4261 acgcaaaatg ttgcattcca acggccccac cgtcgcaaac gcccagcaac tgccgcagct
     4321 ccgctggtcg cgcaccggcg tgcaaccacc gtacgtccgc caatcgaacg ccgccggcag
     4381 cgcccgccgc ggccggcacg gatcaaatgt cgcccccacg cgccagtcct ctggttcgac
     4441 caggccgcaa agttcgctca gactgcgcgc cgtagccgga ttctcgccaa cgctgaaggt
     4501 ccagccctcc cgctcaccac gggcgcacag ctcctcaagt tcgctgcggc tgacttgccc
     4561 ccatgcagga tccacgccgg ctgccacaaa caggaccgcg gcccccgcga ctcgccaaaa
     4621 ccaacccgac agctcgcctg ttctacgggg ccgcatagct tctttgctcc tgcatggcct
     4681 aaagcacgcc cggcaggcac cgcgcgcacc gccgggcaaa gccgctttac ctcagttaat
     4741 ggatgataac cgggtcggtt tgtgcgttca agcgaaactg cgcgcggcca agcactgcgc
     4801 aacaacgctg acacgcgccg tgggcgggga tattattcgc tacatggcaa caagttactg
     4861 ttggggcgaa gtcagcgatc taatggccgc gtaccacgac aacgagtggg gcgtgccggt
     4921 acacgatgat cagcgcttgt tcgaaatact gctgctcgac aacgcccagg ccggtctgtc
     4981 ctgggagaca atcctgcaac ggcgcccgaa ctatcgtaag gcgttcgccg ggttcgaccc
     5041 gcagcgcgtc gcacgcttca cgccgcgcga tgtgcagcgc ttgttgaagg accccggcat
     5101 catccgcaac cgcctcaaga tagagagcgc cattacgaac gccagatgct tcctcgaaat
     5161 ccaggaggag ttcggctcct ttgacgcctt tatctggcag ttcgtccaag gccgtccgat
     5221 tcgcggccgc tggaaaacct ggcgtgatgt gccggccacc acgcccgagt cagacgccat
     5281 gagcaaggaa ctgaagcgcc gcggcttcaa gttcgtcggc tcaaccatct gctatgccat
     5341 catgcagacc gccggcctgg tgaacgatca catactcggc tgcccgcggc gtgcagtttg
     5401 cggctaggcc ttgagcgagc ccggcatcgt ttggtctttg ggcgtcgacc cgccgccgca
     5461 gtagtttgct ctgcgacggc gggctcccgg atcaattcat cacagtcgac ggctctttac
     5521 gacgcccttg gaagctgcaa ccgcaggctc ttgaccggcc gcccagctcc cgggtctggg
     5581 cttagcgccc ggtgggcgat agggggccct gatttaggat ctggccgttc ggcgcgacga
     5641 tcgcgaagaa ctgctgcccg ttctgcaaca tttcgatgcg gaacgcgggc tgcccgtctg
     5701 tggcgccctc gtctacgttg agaatcatgg cgccggggaa agccgaattc acactgctgc
     5761 gaacggtcgc gggcagattc tggaccgtgt cgtccttgta actttgaatg tcactgagga
     5821 tcagaccgtc attacgaacc tcgaggtccc gcagccccgt ggcggttgag actgtcaggc
     5881 tccacgaatc cggcccgctc tgccgcgcat ccacgatcga gcccgcgtga cggctcaaag
     5941 cggcattttg aatggcaggc ggcacggaca atccgctcgg ctgcccgtac atagcggagg
     6001 gccgcaccga actcatctgg gaatagccga ctagagaccc gctcgacgta gggcaggcgg
     6061 agctcggaca ggtcgtctgg tacttgacgg tggttgtcct gtaactgaca ggacaggccg
     6121 tcgggcaggg ctgcgggcac gcggtgaact tctcacggcg tgcgcagagt ccgcacaggt
     6181 tgcaaagggt cgccttctcc ttacaggtcg tcacaccaca ggcgtccgtc ttgcacttgt
     6241 acttgacggg cttggggcat ttcgtctcgc atttgacctt gcaaggctgc gggcatgcca
     6301 tctcgtactt aaccgggcaa ggcttcgggc attttgtctc gcacttgacc gggcagggtg
     6361 ctgggcaaga taccgttttc ggacatctct tacatttgtc acacaacccg ccgaaagccg
     6421 tggaggcgag tgccatgaca gtaatccagc cgacgacgtg cttgatcatc atgcttctcc
     6481 cattttggag ccgctttctc gacagcggcc gttcttcaaa aagggtgcgc tttgacgctc
     6541 attgctaaac cgtcggggcg caatggctta ccgtccacgt cgggtgaacc ggaccacatc
     6601 ccgcgggcat gctcaatact agacgggttc tggaaaaagc tagcggcgac tatcgcaggt
     6661 gtgaatgtaa actcttgtca aaaccacact tacgtcacaa gagacgttgc ccgttggtgg
     6721 acttgaaatc tcgcaaaggc gattgaaagg ccacgttgat ggcgtgagac gcgccagctc
     6781 cgctactcgc ggccgtagtg ctgcagcttc cagaggcgtc gagccgccct ggcacgtgtt
     6841 cgggcggcaa ccaggggttc aagctgccgc cagagcgttt tccaggcccg ccgatccgcc
     6901 ccgaatcgcg ccgtatacgc gcgcagaaag cgcacgcgat ctgccagatt caggccctgc
     6961 acgtccgata agcttacatg cagtgccgcc agcgcacgca gctccccctt cttcgaccag
     7021 cgtcgcgctc gccgcagacc atccatatcg atccaaagca gcttcgattt cggctgaccg
     7081 gccaccaaca cgttgcccgc cttgcagtcg cgatgctcaa agccgcgctc gtgcagccgc
     7141 cgcagatgct gcgccataag gtcggccatc tgtcgtttgc gcatcagcca gacatccggc
     7201 ctgctggcgg cgtgcgtccg cctcaaccag gtttccaggt cgcatgcccc cggaatcgcc
     7261 tcggtgatta ggatgctgtc cagtaccagc ggaccccagc gacgctccaa caccgcaacc
     7321 gggcgcgccg cagccacgtg cctatttaac agcgcatggc ctattcgcca gccgcggacg
     7381 ctgcgcgaca cccccaagag ctgccgcaag cggcgccgcc agtttcgcgc ccgtggccgc
     7441 ttgaaaatga gcggcagcgg ctggttttcg aaagataaca tcccgcgccg cacccaggct
     7501 gaatgtgact ccttgcacga ctcagtttcg ccgttcctga accagcgcag cggatcgcga
     7561 agctgctcac gccaccaggc cggcgtcagc accaggcgtg aaagcggcga gtcggccgag
     7621 gcgtgcttcg tccggatcac ggccatgccg cgccactcgc ccgcgcgcag ccgcgtgaag
     7681 tactggcctt cgcgcatggc ccgccggtcg cgttgcgccc ctaacagctc ggcatgccgc
     7741 tccgcggcgc gcgccagggc ctgcaccagc gagcggaacg aatgtcccag cgggcggccg
     7801 tgctcgaacg cgtgctcgta ctcgttgcgg taacgcaggt acgcgcgcag gaagcgcaag
     7861 cgcgccgtca cgtcgctgcg ccgccggaac cactggttga gctgagccag gttccgcacc
     7921 accgctgcgt cgctcagcgg cactccgcgc cgggcactct gcaagtccac aaaaagcggg
     7981 cggtagcgtc gcggcgcgag cggctgcacc aggatattgg cggcgtgcat gtcgaggtgc
     8041 tcgaagccgg cctggtgcgc ccgcgcgatc atttgtgcca gcgcttttac caactgggcc
     8101 gtgtcacgcc tgcgtcgcgc caggtcatcg tccgcaacaa cctgctccca gaactcgtcc
     8161 agcgcgtaag cgggctccac ggcttccgtc accagcagcg agcagcgcgc accgttcttg
     8221 atcaggtcgg tcgtatagcc ggccggggcc acggccggga tgcccgcttg aagcgcgtaa
     8281 atacccccct tccattccgc ttcgcaggcc ggcccgcgga aaaaccgccg cacgcgcatg
     8341 gcaaagccgt cgccgaaata gtacttgagg tagtaagccg ccccgtccag cctggcgcgc
     8401 cagacttcgc gccgcgcgtt gcgcttcacc cgctgccagc cctgcccctc gggatctgcc
     8461 catgccggcc cgggtacctt ggccagaacc gcgtcgaaac cgttgcgata gacccagtcg
     8521 cgcccgtgga atcgagcgcg tgctgaggcg gtgcgcaccg gttgaacaaa gggcgggtat
     8581 gcgaacgctc cctccaacgg cacggcctgc gcaacagcct tcagtttagc ggtctccatc
     8641 ctctgtaccc agacaccgcg caccctcgac aatgccgggc cattctaagt ccgagtagat
     8701 gtcttctcaa tccgcacccc gagtccgaat cgggcgcacg aaccacaccg ccagagcaga
     8761 gccggggcgt gccgccccga aatgccgcca aaaacctccc atcagcgcag taccagctcc
     8821 gactgcgcgc ggcgccccgc gcccgcccgg cccggcctac gaccgccgat tggtcacgcg
     8881 ccgcagttcc cctatcgctg agcgcagaaa ttgtagctca ccactctcat caaaccctag
     8941 aatttctccc gcaggccaat aatctcgctg aaccagaacg cgcgcgtagc cgtcctccgg
     9001 cggtaatggc cggagcagac agctatgcgt cccgcccgcc agcagcgtcg agaactcgct
     9061 gataagcgcg cccgccgcct cgcgggccac ccacgattcg gccagcactt cgaacggcgg
     9121 actgataaag cccggcccgg gccggaagga gctgcgggag tgctccattt cattcagcac
//
LOCUS       DAOSYS010000146         9146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6450_length_9146_cov_4.959080, whole genome shotgun sequence.
ACCESSION   DAOSYS010000146 DAOSYS010000000
VERSION     DAOSYS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6450_length_9146_cov_4.959080"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLU99_09895"
     CDS             <1..1027
                     /locus_tag="PLU99_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOO17408.1"
                     /translation="LSTISEFGYILGQAARDLDLISAEMLSGLVACLVGSTVLGTLLL
                     PLTPALSEAVDRRLDLRFGGKAPLPVPEHNTPLRSHVLIVGYGLNGRNLARVLRATRI
                     PYSVVEMNPSNAGDARNDGALTVVGDATRLSILQQAGLDTARALVISVAELEPTRRIV
                     AQVHAARPDLYIVTRTRYLSELDTLYQLGARQVIPEEFETSIEIFAHVLKEFGVPDNI
                     IDQQIMLVRAGRYRMLRGLPTDPALRTEWLQILEAAVTQVFLLQAGSPAEGKTIRELD
                     LRARTGATIVALTRSGQPFPAPQPEFRLEAGDVLVLVGRHKQLDEARRMLEAPPEYEA
                     SPAATVT"
     gene            1251..1616
                     /locus_tag="PLU99_09900"
     CDS             1251..1616
                     /locus_tag="PLU99_09900"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOO17409.1"
                     /translation="MRLVCAADGYKAPARSEAEACRPKDFEISANNDQSREDRPNPSP
                     LRYANLGLELAAAIVGLTLVGLWFDHHFGTGPVGVLVGAGLGVVGGLCNFIRAALKMN
                     QDGPARRGGQDEFDRRDHR"
     gene            1603..2031
                     /locus_tag="PLU99_09905"
     CDS             1603..2031
                     /locus_tag="PLU99_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17410.1"
                     /translation="MITDSPRTFGPASAYGRLAAFSLGGVLLLVAVGYLPTRAMGGGP
                     ALVAMAVGLGIALVAALAGLAPPIIWAAGSPRAQVTGVLIGMGLRFVLTLGLTLAALL
                     SRMLPPVGLAVWVVIAYLVLMTIDTIGLVRLTRSNARANA"
     gene            2028..2957
                     /gene="atpB"
                     /locus_tag="PLU99_09910"
     CDS             2028..2957
                     /gene="atpB"
                     /locus_tag="PLU99_09910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOO17411.1"
                     /translation="MTGLCLGQLNPLEHVVQHRIWAPHLPWDIPFFDQFTILSNHMIM
                     QVIAAVLVVTLLPRFVRKRAGDDPIGRFVPRGVGNAIEAICGLLREHVARPALGPHTD
                     RFVPYVWSAFFFVLTCNLLGLIPLSDWTRPLGVSGLGGTATGNIGVTAALAICTLGMI
                     IINGLRLQGWGYIRHFFMGPPLLAPMIAVLEVVGLIAKTFALAMRLFANMIAGHILLA
                     VLLGLTGMAWAALGAGWGSGVAGLVVLGSGFIYCLELLVAVLQAFIFAFLSATFIGQA
                     VVLHAEHTEHAAEAHADLHATAEHPEKSASEGA"
     gene            3145..3453
                     /gene="atpE"
                     /locus_tag="PLU99_09915"
     CDS             3145..3453
                     /gene="atpE"
                     /locus_tag="PLU99_09915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOO17412.1"
                     /translation="MLATLSAVPVSAAEPADAGAAVSKPIFGDPGLKALGAGIGAGLV
                     LIGGAAGIGRIGGSAVESMARQPESAGTINTAMIITAAMIEGATLFGVVVAMLALFIG
                     "
     gene            3552..4154
                     /gene="atpF"
                     /locus_tag="PLU99_09920"
     CDS             3552..4154
                     /gene="atpF"
                     /locus_tag="PLU99_09920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOO17413.1"
                     /translation="MLKRYPMPMLASALLFGGAPLAWAAEHGEETNIFNADIGNFILT
                     LIIFGLVVYILGKFAWKPVLTVLKERERNIRESLEAARREREAAEGLLKEYQARLEKA
                     RQEASAIVEEGRRDGEVVRRRIQDEARQQANEMIERARREIRLATDAAVKELYDQTAE
                     LAVKLAGGIVRKELSPGDHRRLIAESLDRMRSADRAKIKQ"
     gene            4222..4797
                     /gene="atpH"
                     /locus_tag="PLU99_09925"
     CDS             4222..4797
                     /gene="atpH"
                     /locus_tag="PLU99_09925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOO17414.1"
                     /translation="MTIKGSDIAKVYADALFDLAKAAGTIDKIGAELQELASFQVQEP
                     QFAEFLSSQVIDDDERERSLERMFRGRVDDTLLDTLQVMNQHGRIGLLPQLLRAFVLR
                     IEEDRGQVEVRAISAVELSGVEQADVQTLAAQLTGRQPLMDYRIDPSIIGGLVLEVGG
                     YRYDDSVRHNLDALRRRLLERSSRGLKIGVT"
     gene            4866..6563
                     /gene="atpA"
                     /locus_tag="PLU99_09930"
     CDS             4866..6563
                     /gene="atpA"
                     /locus_tag="PLU99_09930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOO17415.1"
                     /translation="MKFKADEIASVIREEISRYRAALDVSQVGRVLEVGDGVARVYGL
                     PQAMAGEMLEFESGAVGLVFNLEENSIGTVVLGDYIDIEEGDEVRATGRLLSVPVGDA
                     IVGRVVDPLGRPLDNKGPVISPHRRPLEIIAPGIAERQPVNEPLQTGVKAIDSMIPIG
                     RGQRELIIGDRKTGKTAIAIDTILNQRGGDVICVYVAIGQKESTVASIVETLRKHDAM
                     DYTIVVTAGASDPAPLQYIAPYAGCAMAEYFMYEHGRATLCVYDDLSKQAQSYRQLSL
                     LLRRPPGREAYPGDVFYLHSRLLERACKLAELRIIVPKTAPPETKQGVNNKVYKGEQD
                     LEALKTDLEALPDRDRLEVRKVPGSGGSLTALPIIETLEGEVSAYIPTNVISITDGQI
                     YLEPELFFAGVRPAINVGISVSRVGGKAQVGAMKKIAGSLRLDLAAFRELEAFAQLGT
                     ELDAATQRSLDRGRRMVELLKQPQYEPMHVADEILSIHAGVRGFLDDLPLDQVRPFEQ
                     ELLKYYRDEYPELRERVLKDKWTDELDERLKEIVKQCKAAFLANLKKAEAAGASSHG"
     gene            6556..7431
                     /gene="atpG"
                     /locus_tag="PLU99_09935"
     CDS             6556..7431
                     /gene="atpG"
                     /locus_tag="PLU99_09935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOO17416.1"
                     /translation="MAKARAVVKRRRAVQNIRKITETMQLIATARYQKAYQRATATQP
                     YTAHIREMLASVAQQAGLDDPLLKPNAGVPRSLMLVITSNRGLAGAYNASVLRTAIHR
                     RAELVQAGITPDLEVVGKKGIAYFRFLGFPITTRITDVDDRIAFARVADMAERYMNAY
                     RNGEIARVDVAYMRFHSMASQRPAALQLLPIEPPSAPESEAARAQAAEFEFSPPAPEL
                     LARLVPETVKTVLFQCFNDALLSEQVARMVAMKAATEAAGDMIKYLTRQYNRARQAQI
                     TLELADIVGGAEAVK"
     gene            7439..8896
                     /gene="atpD"
                     /locus_tag="PLU99_09940"
     CDS             7439..8896
                     /gene="atpD"
                     /locus_tag="PLU99_09940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOO17417.1"
                     /translation="MVDNGENWGRVSQVIGSTLDAEFDEKRLPKIYNALQVKVRRPVG
                     DTDVEETLWCEVAQQLGGGRVRAIALGSTDGLVRGSKILDTGAPIKVPVGPATLGRVF
                     NLVGEPVDGRGPVETSDWRPIHHDPPEFADLTPKTEQFETGIKVIDLLVPFVRGGKIG
                     LFGGAGLGKTVVIQELIARIATQHGGYSVFAGVGERTREGNDLWLEMQQAKIGDTGKS
                     VIDQTAMVFGQMNEPPGSRLRAALSALTMAEYFRDESGADTLLFIDNIFRFSQAGSEV
                     SALLGRMPSAVGYQPTLGTEMGELQERITSTRQGAVTSVQAVYVPADDLTDPAPATTF
                     THLDAFIVLERSISEKGIYPAIDPLASSSRILDPAVVGEEHYAIARRVQEILQRYKSL
                     QDIIAILGVEELSEEDKLVVARARKIERFLSQPFFVAETFTGFPGNYTSKADTIRSFK
                     ELCEGKWDHLPEQAFMYVGAIEQAAEKAERLKAGA"
     gene            complement(8907..>9146)
                     /locus_tag="PLU99_09945"
     CDS             complement(8907..>9146)
                     /locus_tag="PLU99_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase"
                     /protein_id="HOO17418.1"
                     /translation="QHGVGRSALLAACTLVALGMDCREALQVLKRRRPTVAPSPEQLQ
                     ALLDFAARSAETPAPGKPATLDDLFQIAYAGLEVG"
BASE COUNT         1681 a         2810 c         3050 g         1605 t
ORIGIN      
        1 gttgtcgacc atcagcgagt tcgggtacat cctcggtcag gcggcccgcg acctggacct
       61 gatatcagcc gagatgctga gcggactggt cgcctgcctg gtcggctcga cggttttggg
      121 caccctgctg ctgcccctaa cgccggccct gtccgaagcg gttgaccggc gtctggacct
      181 gcgcttcggc ggcaaagctc cccttccggt gcccgagcac aacacgccct tgcgcagcca
      241 cgtgctcatc gtaggctatg gcctgaacgg gcgcaatctg gcgcgagtgc tgcgagccac
      301 ccgcatcccc tatagcgtgg tggagatgaa tcccagcaac gcgggcgatg cgcgcaatga
      361 tggcgcactg acggtcgtcg gagatgccac gcgcctctcc atccttcagc aggccggcct
      421 cgacaccgcg cgggctttgg tgatttcagt cgccgagttg gagccgacgc ggcggatcgt
      481 cgcgcaagtg cacgcggctc gcccggatct gtacatcgtg acgcggacgc gctatctctc
      541 ggaactcgac acactctacc agctcggggc acggcaggtc attcccgaag aattcgaaac
      601 ctccatcgag atcttcgcgc acgttctcaa ggaattcggc gtccccgata acatcatcga
      661 ccagcagatc atgttggtgc gcgccggccg ctatcgcatg ctgcgcggcc tgccgaccga
      721 tccggctctg cggaccgagt ggctgcaaat tctcgaagcc gccgtgacgc aggtcttttt
      781 gctgcaggcc ggcagcccgg cggagggcaa gaccatccgc gaactcgacc tgcgcgcccg
      841 aacaggagcg acgattgtgg cgttgactcg ctccggccag ccctttcccg cgccccaacc
      901 cgaattccgc ctggaagccg gcgacgtctt ggtactggtg ggcaggcaca agcaactgga
      961 cgaggcccga cggatgctcg aagcaccgcc cgagtacgaa gccagcccgg cagcgaccgt
     1021 cacttgagtc ggttatcgga tgttcgcacg ctggaaaccg cttccggcgc actcaacccg
     1081 gaccggcagt cagcgacgcg cgaaactcgc gcaacgacgg tgccacacgc aaacctgtcc
     1141 ggaatgacgc tatcagccct cggtttccag ttcgaacccc caggccgccg gcgtcgtcgg
     1201 cgcccttaac cgtgctggcg tgcttgacgt attggctccg aacgggctaa atgcgcctcg
     1261 tttgtgctgc ggatgggtac aaggcgccgg cgcggtccga ggccgaagcg tgccggccaa
     1321 aggatttcga aatctccgcc aataacgacc agagccggga agaccgcccg aatcccagcc
     1381 cgcttcgcta tgcgaacctc ggcctggagt tggcagcggc gatcgtcggc ctgacgctgg
     1441 tgggcctgtg gttcgatcac cattttggca ccggaccggt cggcgtgctc gttggagccg
     1501 ggctgggagt cgtaggcggg ctatgcaatt tcattcgtgc agcgctgaag atgaaccagg
     1561 atggtcccgc ccggcgcggc gggcaggacg agttcgaccg ccgtgatcac cgatagccca
     1621 cgcacgttcg gcccagcaag cgcctacggg cgtctagccg cattctccct gggcggcgtg
     1681 ctgctgctgg tcgcggtcgg atacctgccg acgcgggcca tgggcggcgg gccggcgctt
     1741 gtggcgatgg cggtgggctt gggcatagcg ctggtcgctg cgcttgcggg actggcgccc
     1801 ccgataatat gggcagcggg cagtccgcgg gcccaggtca ccggggtcct aatcgggatg
     1861 ggcctgcggt tcgttctgac gctcggcctg acgttggccg ccctgctgag tcgaatgctg
     1921 ccgccggtgg gactggcggt ttgggtcgta atcgcctacc tggtcctgat gaccattgac
     1981 accatcggcc tggtccggct gacccggtcg aatgcgaggg caaatgcgtg accggtctgt
     2041 gcttgggaca attgaatcct ctcgaacacg tcgtgcagca ccgcatttgg gcgccgcacc
     2101 tgccgtggga cataccgttc ttcgaccagt tcacgatcct gagcaaccac atgatcatgc
     2161 aggtaatcgc agccgttctg gttgtgacgc tgctgccgcg cttcgtgcgg aaacgcgccg
     2221 gggatgaccc gatcggccgg ttcgtaccgc gcggcgttgg caatgcgatt gaggcgatct
     2281 gtggcctgct tcgcgaacat gtggcccggc cggcgctggg ccctcacacg gaccggttcg
     2341 tgccctatgt ctggtcggcg ttctttttcg tcctgacctg caatttgttg gggctgatac
     2401 cgctgagcga ctggacccgc ccactgggtg tcagcggcct gggcggcacc gccaccggca
     2461 acatcggcgt gacggccgct ctggcaatct gcaccttggg catgatcata atcaacggct
     2521 tgcggctgca gggctggggc tacatccggc atttcttcat ggggccgccg ctgctggcgc
     2581 ccatgattgc ggtgctcgaa gtggtcggac tgatcgcgaa gacgttcgcg ctggccatgc
     2641 gtctcttcgc gaatatgatc gccggccata ttctgctggc ggttctgctg ggcctgacgg
     2701 ggatggcgtg ggcggcgttg ggggcgggtt gggggtcagg cgtcgctggg ctggttgtgc
     2761 tcggcagcgg tttcatatac tgcctggagt tgttagtggc agtgctacag gcgtttattt
     2821 tcgcgttcct cagtgccacg ttcatcgggc aggcggtggt cctgcacgct gaacacacgg
     2881 agcacgcggc cgaagcccat gctgatctgc acgcgacggc cgaacacccc gaaaaatcag
     2941 cttctgaggg agcatgacgc gcttgaggca cgagcagcga aacgaagatt actcgccatg
     3001 cccggtaagt ggggtaacgg gccgtaaggc tggaatatgc gatggcgatc tttggacaag
     3061 agccgggcgg ccccaccgcc ggcatgcaca cacagcgagg tggtaattat gcggtctatc
     3121 aggataattg cagttgcggg gctggtgctg gcgacgctta gcgcggttcc ggtcagcgcg
     3181 gccgaacccg cggacgcggg cgcggccgtc agcaagccga tctttggcga tcctggctta
     3241 aaggcgctgg gcgcggggat cggggcggga ttggtgctga tcggtggagc ggcggggatt
     3301 gggcgcatcg gcggcagtgc ggtggaatcc atggcgcgcc agccggaaag cgccggcacg
     3361 atcaacacgg ccatgatcat cacggccgcg atgattgaag gcgccacgct ctttggcgta
     3421 gtagtggcca tgctggcgct attcattgga tgagaatctg caccggcgcg ggtcagcgcc
     3481 gggcacagag gcggcttcag agccccggcg gccgcgcggc ggcagagaaa cagctcctga
     3541 aggagaagat tttgcttaag cggtacccga tgcccatgtt agccagcgcc ctgctctttg
     3601 gcggggcgcc gctggcgtgg gcggccgaac acggtgaaga gaccaacatc ttcaacgccg
     3661 acatcggcaa tttcatcctc acgctcatca tcttcggcct ggtcgtatac attctgggca
     3721 agttcgcctg gaagccggtg ctgaccgtac tgaaggagcg cgaacgcaac atccgcgaat
     3781 cgctcgaagc ggcgcggcgt gagcgcgagg cggccgaggg actgctcaag gaatatcagg
     3841 cccgcctgga gaaggcccgg caagaggcgt ccgccattgt tgaagagggg cgccgcgacg
     3901 gtgaagttgt gcggcgccgc atccaggatg aggcgcgcca gcaggccaac gaaatgatcg
     3961 agcgggcgcg gcgcgaaatc cgcctcgcga ctgatgccgc ggttaaggaa ctttacgatc
     4021 agacggccga actggcggta aaactcgcgg gcggaattgt gcgcaaggag ctgtcgccgg
     4081 gcgatcatcg gcggctgata gccgaatctc tggaccggat gcggtccgcc gaccgggcga
     4141 agatcaagca gtagcaggcc gcggcttgca aaccgaggcc gccggcgtct caccggccgg
     4201 cgcactggaa gatgaaacgg catgacgatc aaaggcagcg acatcgcgaa ggtttacgca
     4261 gacgccctgt tcgacctggc caaagcggcc ggcacgatcg acaaaatcgg tgctgagctg
     4321 caggagcttg cctccttcca ggtgcaggag ccgcagttcg ccgaattcct gagttcccag
     4381 gtgatagacg acgacgagcg tgagcgcagc ctggagcgca tgtttcgcgg acgggtggac
     4441 gacaccctgc tcgacaccct tcaggtgatg aaccagcatg ggcgaatcgg gctgctgccg
     4501 cagcttttgc gggcgttcgt gctgcgcatc gaggaggatc gcggccaggt cgaggtgcgg
     4561 gccatcagcg ccgttgaact ttccggggtg gagcaagcgg atgtgcagac tttggcggcg
     4621 caactcaccg gcaggcaacc gctgatggac tatcggatcg acccgagcat catcggcggg
     4681 ctggtgctcg aagtgggagg ctaccgttac gatgactctg tccgccacaa cctggacgcc
     4741 ttgcgccgcc ggctgctgga gcgcagcagc cgcggtttga aaatcggcgt cacatgagca
     4801 gtgatcgccc atgcaggttc gccctgcgga ccactagttg atagcttgaa agctgagagc
     4861 ttctaatgaa attcaaagca gacgagatcg ccagcgtcat aagagaagag atcagccgtt
     4921 accgcgccgc gctggacgtg tcccaggtgg ggcgcgtgct ggaggtcggc gacggcgtgg
     4981 cccgcgtcta cgggctgccg caggcgatgg ccggcgagat gctcgaattc gagagcggcg
     5041 ccgtcggact ggtcttcaac ttggaagaaa attcgatcgg caccgtcgtg ctgggcgatt
     5101 acatcgatat cgaggaaggc gacgaggtgc gcgccaccgg ccgtctgctg agcgtgcccg
     5161 tgggcgatgc gatcgtcggg cgcgtggttg atccgctggg ccgcccgctg gataacaagg
     5221 gcccggtcat ttcgcctcac cgccggccgc tcgaaatcat cgcgcccggc attgccgagc
     5281 gtcagccggt aaacgagccg ctgcaaaccg gcgtgaaggc catcgacagc atgattccca
     5341 tcggtcgcgg acagcgcgag ctgatcatcg gcgaccgcaa gaccggcaag acggccatcg
     5401 cgatcgacac cattttgaac cagcgcggcg gggacgtgat ttgcgtctat gtcgccatcg
     5461 ggcagaagga atccaccgtc gccagcatcg tcgagacctt gcgcaagcat gacgcgatgg
     5521 actacaccat tgtggtgacg gccggcgcct ctgacccggc tccgttgcag tacatcgcgc
     5581 cgtacgcggg ctgcgccatg gccgagtact ttatgtacga gcatggccgg gccacgctgt
     5641 gcgtctacga cgatctttcg aaacaggccc agtcttaccg gcagctctca ctgctgctgc
     5701 ggcggccgcc cgggcgcgag gcatacccgg gtgacgtgtt ctacctgcac agccggctgt
     5761 tggaacgggc gtgcaaactc gccgagctgc ggatcattgt gccgaagacg gccccgcctg
     5821 agacgaagca aggcgtcaac aacaaggttt ataagggcga gcaggacctc gaagccttga
     5881 agaccgatct ggaggcgctg cctgacaggg atcgactgga agtacgaaag gtccccggca
     5941 gcggcggcag cctgaccgcc ctgccgatca tcgaaacgct cgaaggcgag gtctcggcct
     6001 acatccctac caacgtgatc tcgattaccg acgggcagat ctacctggag ccggaactgt
     6061 tttttgccgg cgtgcgcccg gcgatcaacg tgggtatcag cgtcagccgc gtgggcggca
     6121 aggcccaggt tggagcgatg aagaagatcg cgggctcgct gcgtctggac ctggcggcgt
     6181 tccgggaact ggaggcgttt gctcagcttg gcactgagtt ggacgcggcc acgcaacgtt
     6241 cgctggaccg cgggcgccgc atggtggagc tgctgaagca accccaatac gaaccgatgc
     6301 acgtggccga cgagatactc tccatccacg cgggcgtgcg cggcttcctg gacgacctgc
     6361 cgctggacca ggtgcggcca ttcgagcagg agttgctgaa gtactatcgc gacgagtacc
     6421 cagagctgcg tgagcgtgtg ctcaaggaca agtggacgga tgagctggac gaacgcctga
     6481 aggagattgt gaagcagtgc aaggcggcat ttctcgcaaa tttgaagaag gccgaagcgg
     6541 ccggggcgtc cagccatggc taaagcgcgc gcggtagtca agcgccggcg ggccgtgcag
     6601 aacatccgta agattacgga gacgatgcag cttatcgcca cggcgcgcta ccagaaagca
     6661 taccagcgcg ctacggcgac acagccgtac acggcgcata taagagagat gctcgcgagt
     6721 gtggcacagc aggccgggct ggatgacccg ttgctcaagc ccaatgcggg cgtcccgcgc
     6781 agcctcatgc tcgtgattac gtcgaatcgc ggcctggccg gagcgtacaa cgcctcggtt
     6841 ctgcgaacgg cgattcaccg ccgcgccgaa ctggtgcagg ccggcattac gccggatttg
     6901 gaagtggtcg ggaaaaaagg catcgcctac ttccgctttc tgggttttcc gatcacgacg
     6961 cgcattaccg acgttgacga ccggatcgcc tttgcgcgcg tggctgacat ggccgaacgg
     7021 tacatgaatg cctatcgaaa tggggaaatc gcgcgggtcg acgtcgcgta catgcggttt
     7081 cactcgatgg cgagccagcg cccggcggcg ctccaattgt tgccgattga gccgcccagc
     7141 gcgcctgaat cagaggccgc ccgcgcgcag gccgctgaat tcgagttttc cccgcccgct
     7201 ccggaactgc tggcgcggct tgtgccggaa accgtcaaga cagttctctt ccagtgcttt
     7261 aatgacgccc tgctgagcga acaagtggcg cgcatggtgg caatgaaagc cgccaccgag
     7321 gcggccgggg acatgatcaa gtatttgacc aggcagtaca accgcgcccg ccaggcccag
     7381 attacgctgg agctcgcgga catcgtcggc ggcgcagaag cagtcaagtg agatggctat
     7441 ggtagataac ggcgaaaatt ggggccgcgt ttctcaagta atcggttcca ctctcgacgc
     7501 ggaattcgac gagaagcgtc tgccaaaaat ctataacgcg ctgcaagtaa aggtgcgccg
     7561 gccggtgggc gacacggacg ttgaggaaac gctctggtgc gaggtcgccc agcagcttgg
     7621 cggcggacgg gttcgcgcga tcgccctcgg cagcaccgac ggcctggtgc gcgggtcgaa
     7681 gattctcgac accggcgccc cgatcaaagt tcccgtcgga ccggctacgc tggggcgcgt
     7741 cttcaacctc gtgggtgagc cggtcgacgg ccgtggcccc gtggaaacca gtgactggcg
     7801 ccccattcac cacgacccgc ccgaattcgc ggaccttaca cccaagaccg agcagttcga
     7861 gaccggcatc aaagtcatcg acctgctcgt gcccttcgtg cgcggcggga aaatcggcct
     7921 gttcggcggc gccggcctgg gaaaaacggt cgtcattcaa gagttgatcg cccgcatcgc
     7981 cacgcagcat ggcggttact ccgtcttcgc cggcgtcggt gagcgtaccc gcgaaggcaa
     8041 cgacctttgg ctcgaaatgc aacaggccaa aatcggcgac accggcaaat cggtcatcga
     8101 tcagaccgcc atggtgttcg gccagatgaa tgagccgccc ggctcgcgcc tgcgggccgc
     8161 gctgagcgcc ttgaccatgg ccgagtactt ccgcgacgag agcggagccg acacgctgct
     8221 gttcatcgac aacattttcc gtttctcgca ggccggctcg gaagtctcgg ccttgctcgg
     8281 ccgcatgcca tcggccgtgg gctatcagcc gacgctgggc accgagatgg gcgaacttca
     8341 ggagcggatt acgtctacgc gccagggggc cgtcacgtcg gtgcaggcgg tctacgttcc
     8401 ggccgacgac ctgaccgacc ccgccccggc cacgacgttc acccacctgg atgcgttcat
     8461 cgtgctcgag cgttcgattt ccgaaaaggg tatttacccg gcaatcgacc cgctggcctc
     8521 ttcgagccgc attctggacc cggccgttgt cggcgaagag cattatgcca tcgcgcggcg
     8581 cgtgcaggag atcctgcagc gatacaagag cttgcaagac atcattgcga ttctgggcgt
     8641 cgaggaactt tcggaagagg acaagctggt cgtagcccgc gctcgcaaga tcgagcggtt
     8701 tctgtcgcag ccgttcttcg tggccgagac gttcacgggt tttccgggca actatacatc
     8761 caaggcggac acgatccgca gcttcaagga attgtgcgag ggcaaatggg accatttgcc
     8821 cgaacaggcg ttcatgtacg tcggcgctat cgagcaggcg gcggagaagg cggagcgtct
     8881 gaaggcgggc gcatagcaga cgcggcttag ccgacctcca gcccggcgta agcgatttgg
     8941 aacaggtcgt ccaatgtggc cggcttgccc ggggccggcg tttcggcgga ccgcgcggcg
     9001 aaatcgagta acgcctgcag ttgctccggg ctgggtgcaa cggtcggccg ccgccgcttg
     9061 agcacctgca acgcctcccg gcagtccatc cccaaagcaa ccagcgtgca cgcggccagt
     9121 agcgcgctgc gcccgacgcc gtgctg
//
LOCUS       DAOSYS010000147         9066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6532_length_9066_cov_4.235934, whole genome shotgun sequence.
ACCESSION   DAOSYS010000147 DAOSYS010000000
VERSION     DAOSYS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6532_length_9066_cov_4.235934"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(600..1952)
                     /locus_tag="PLU99_09950"
     CDS             complement(600..1952)
                     /locus_tag="PLU99_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17419.1"
                     /translation="MFTQLPFRALLIVGALALLGWAPLSTAVAMDWEIESFDRLVLWF
                     GDGVSIAVDQDGNPYIAHDVSLEGMIHFAFRGPEGWVIEAVDDYYSSGGGSAVALDAS
                     GTPCIVYGDGNPDIEPVVLTFARRTPSGWEREAVDQMRYDSHVALRFRSDGLAVIAYS
                     AEGGGQLKYASQTAGGSWDVEIADAVPASGCDMSLVLDENDDPHLCHWESTSGRPRQV
                     YTCRKDGLWLRHILDAVGMCNAMGSGIDVDGQGKVHIAWQIHECESPGALYYAKLSEA
                     GSSTAKVDSDFSFYSAGCSLVVDRLGRPHILYGTQGQLVGGPSELRYAHLDEEGKWVH
                     ELVDDDGDCGEINAVTIDADGFLHVAYYAGDGSTQFGVIRYARSATPVAPPPLPGDLD
                     CDGMVNAFDIDPFVLALTDEAAYAAEFPTCDYMAADTNQDGEVNVFDVDPFVALLTGS
                     "
     gene            2531..3793
                     /locus_tag="PLU99_09955"
     CDS             2531..3793
                     /locus_tag="PLU99_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOO17420.1"
                     /translation="MSKVAILRTSPATVLRDYHELLNLADYQQTIAREAETALKINIS
                     WHFFYPGSSTTPWQLEGVIRAMKADGYDPQRIHACHNRTVVIDAHLGERENKQLNVVE
                     AHGLRNVHLYEGEEWINVRDAVGDLTRQFLCLNEVYPRGFNIPRRFIGENIIHLPTTK
                     THVFTTMTGAMKNAFGGLLNERRHWTHPVIHETLVDLLMIQKKIHRGVFAVTDGTFAG
                     DGPGPRCMVPYVKNVILASADQVAIDAVAAKLMGFDPLSIKFIRLAHERGLGCGDPQK
                     IEIVGDRQAAEENWHFAGPFQKMTFASRMQHKIYWGPLKGPIEWSLKTVLAPWAYMAS
                     VLYHDSFWYPVRAKKLMADVLSSDWGRLFRNWENARPDANGYPDLGVAPAELHRTGWR
                     AFAKSIGILGTCIKEAPEFSTRRRPVAK"
     gene            complement(3860..4963)
                     /locus_tag="PLU99_09960"
     CDS             complement(3860..4963)
                     /locus_tag="PLU99_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO17421.1"
                     /translation="MNARVDVLNAGSNVFVTGATGLIGGELVRALVQQPVGKVWTLIR
                     PSPDADPAVRLVQRLRRSGSSDGTLGVHAVAGDVRLPHLGLAADDLDEIRRDADIIIH
                     SAAETSFIRAAACRQTNIAGAQNLIEFARSARRRPLLVCLSTAYVSGAVANVCLSETS
                     GLGDAHHNEYTRSKAVAEDLFRSSGLPVLVVRPSIVLSAGLPDEQFARAILGFLPWST
                     VFEAVPIDPAARADTVPVQFVVQSIVRLLQVPRRKHDCYHISAGTQGSACYAEFSAVI
                     DRFYGRARPLQLIHPSQWTRELHRRCIATPQQRRVFGQLRNYLPFLNMNVLYDNARLR
                     EELGDKFPELPPLASYFGELLALMAKPRLTAAS"
     gene            5406..6626
                     /locus_tag="PLU99_09965"
     CDS             5406..6626
                     /locus_tag="PLU99_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17422.1"
                     /translation="MKAAPLTIALLCICLLLTLTPGGCPLRGGSTNQNDNAAANGNLN
                     SDDAGRNANSADGNVNGAANANDNAAVNANDNSAPPPIAPGFWEIVSGDLLQNGPVDL
                     AYMALNADHTVDFHYYMPELNIAVGQTGIYSVAGSVLLLGFGTPPTPVMLVFDQPDAD
                     TLHLMSDGGDELVLSRAAEIPEQFQCLPLTPVHTYTGLPRPDERTGMAYDGSRLWYTT
                     DASVLQAIDPAHGTPGETHVLTTPLVHAYQGGAFWVVSGSGAMEQAERRRLDNTLLDE
                     IDTRVDLGEANRIEVLAWESRDNVLWVHGSLPDHSADRFLRVACLGEPDELLGIDEFD
                     VAISAMSWHGRELWAIVSGMVVQLDMTALRGEVTYRVDLPGIRWRGIAWAGDAMFLIG
                     ANDDGGVLVEVDPG"
     gene            6788..7087
                     /locus_tag="PLU99_09970"
     CDS             6788..7087
                     /locus_tag="PLU99_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17423.1"
                     /translation="MRLIRELAADPDRVAVYRTAKYDFLAPTLTVDDVCDAICEWIDR
                     GNPVIETVIHTIPERKNTPAYELKPVLCEKRYYVKLALERQGEGWLLILSAHLDV"
     gene            7109..7687
                     /locus_tag="PLU99_09975"
     CDS             7109..7687
                     /locus_tag="PLU99_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOO17424.1"
                     /translation="MGSVCLTCGQGTMEPKRGTFRFDPPPNIPGGTIEIENAEWRECT
                     ECGEQILGRELDRRLDDAARHRLGLLSPAEIKEIRERIGLSQEAMAQWLGVGDKTYTR
                     WETGKSIQNKSNDTLIRLVARNAGIFSELDAEREPDRSKRIAEYVHSLHDLKGESPLA
                     MAAHGGELPERVVEELRQRLRAILEFQRNRKG"
     gene            7693..8532
                     /locus_tag="PLU99_09980"
     CDS             7693..8532
                     /locus_tag="PLU99_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOO17425.1"
                     /translation="MEFKASYLEIEPRELIEFLLRESGHADREAVNPKSILDVLRLEH
                     VTANFAAELPPELTQRSTPVRGMLSYRDRIVATDINLNGTRARFSVLHEVAHYVLPTH
                     QHALYVCDETGMSFRTRVTIEQEANAFAAELLFLGDRFTSEVNSRPLTAATIKLLAQK
                     YQASFEATARRMIERSLRPCMLVVCDNTGSRVDPDAQPVWSKKYTIASVPFRTKYFSD
                     LHSAVIPPDVAAQVLAPGRDIADSVTHEVTIRTATGTDARFRAEFFSNTYSIFCLLRP
                     TAN"
BASE COUNT         1816 a         2993 c         2686 g         1571 t
ORIGIN      
        1 aagtgcctga agcctttctc ctgcgtacgg cacgcgagcg caaacagact gatccgcgat
       61 cgtcgagccg tacgattccg atgtcccggg aacgcgtccg ccggcctgag ccgctaaacg
      121 gttccgttcc ttcgttcact gccgtgcacg gcgtggcaga accaaagttg ccttcactct
      181 atagtcacta taacccaaga atcgcgtcca tgcaaatttc tatttggaat aatttctgga
      241 tttcttggag aagccgccgc accgctgcga gattcctgaa aaatgctcag cgaatggttg
      301 aaccgccgcg cctcggaaat tcatcgcaag cgccagaacg acaacgactt ataactattg
      361 gccacggact tgcaccgact gccccgcccg caccggcaac ttatcggtca tagatgagcg
      421 cgagcgcgga gcaacgctca agctgcccgc gctcgctctt gatgcgcctc gccgctaaaa
      481 ataccgcata gtattcgcga ttagtcctgg cgtccgtcac cttcccacgt tcccactttc
      541 ccaccttcgc acccgtggga aagggggcgt caaatgccgg tcaccaacgc cggcaagcgt
      601 cagctacccg tcaacaaggc tacgaacggg tccacgtcga acacgttgac ctcgccgtcc
      661 tgattggtgt ccgccgccat gtagtcgcac gtcgggaact ccgccgcgta cgcggcctca
      721 tcggtcagcg ccagcacaaa cgggtcgatg tcaaatgcgt tgaccatccc atcgcaatcg
      781 agatcaccgg gtagcggcgg cggcgcgacc ggcgtcgcgc tgcgcgcata gcggatcaca
      841 ccaaactgcg tggagccgtc gcccgcataa taggccacgt gcagaaaacc atcggcgtcg
      901 atcgtgaccg cgttgatctc gccgcagtcg ccatcgtcgt ccaccagctc gtgcacccac
      961 ttcccctctt cgtccaaatg cgcatagcgc aattccgagg ggccgcccac caattggccc
     1021 tgcgtgccat agagaatgtg cggccggcca aggcgatcaa ccaccaggct gcacccggcc
     1081 gagtagaagc tgaagtcgga atcgactttg gctgtactcg acccagcctc cgacagcttg
     1141 gcataataca acgcgcccgg tgactcgcac tcatggattt gccaggcaat gtgcaccttg
     1201 ccctgaccgt ccacgtcgat tccactgccc atggcgttgc acatgccaac cgcgtccagg
     1261 atgtgacgga gccatagccc atctttgcgg cacgtgtaga cctggcgtgg ccttcccgag
     1321 gtcgactccc agtggcagag gtgcgggtcg tcattctcgt ccagtaccag cgacatgtcg
     1381 catcctgagg ccggaaccgc gtctgcaatc tcgacgtccc atgagccgcc cgcggtctgt
     1441 gacgcgtact tcagttgccc cccgccctcg gcgctgtagg ctatcaccgc cagcccatcc
     1501 gaacggaacc gcagcgccac atgcgagtcg tagcgcatct ggtcgacagc ctcacgctcc
     1561 cagccggacg gcgtgcgccg cgcaaatgtt aagacgacgg gttctatatc cgggttgccg
     1621 tcgccgtaaa caatgcaagg ggtgccggac gcgtccagcg cgaccgccga ccctccgccg
     1681 ctggaatagt agtcatctac cgcctcaatg acccagccct cggggccgcg gaatgcgaaa
     1741 tgtatcatcc cttcaaggga cacatcgtgc gcaatgtacg ggttcccgtc ctggtctacc
     1801 gcgatcgata ctccgtcacc gaaccacaag accagacggt cgaacgactc gatctcccaa
     1861 tccatcgcca ccgccgttga cagcggcgcc cacccgagca gcgccaacgc tccaacaatc
     1921 aataatgccc tgaatggaag ctgtgtgaac atttgatttc ctcctgtgct ccagcgcacg
     1981 ggataccgcc gcctggtcag ggccggtctg ccccgagcgc aggaattctt atgcctttac
     2041 tgcaaccggt tcagtccttg ttacgaagcg atcttggtca gaccggtccg gcttgcaggc
     2101 gtgccaaccc gcgctgccgc agcggactcg tgaccactac taaaaaggac ccgcccgccg
     2161 ctacagccgc gcgggccgcg ccatcagggt gtggttgcac aaaatcggga gctcatgact
     2221 cgcctcctat gtaagggcca atcgaacagc gtgcgtgcgg aagtgccaac cgcatgcagt
     2281 cgcgcgtgcc accgcgaccg atacgcacgc ttgaccacta gtcataagtg taacacaaaa
     2341 aagagcagcc gggtgaaaag tatgctcaaa agaagcagcg gcgaggcgcg ccgcccggaa
     2401 accagagcac agccgggcgc gccgcccccg aatgccggga cagggcatcg ggagggggcc
     2461 gttgagccgc ccctgtcgcc cgcgcctgta agcctctaaa atcccggaaa cgagccaaca
     2521 ggaattctag atgtcgaaag tcgccattct tcgaacgtcg cccgccacgg tgctacgcga
     2581 ttaccatgag ctgctgaacc tggccgacta ccagcaaacc atcgccaggg aagcggaaac
     2641 cgccctcaag atcaacatct cctggcactt cttctacccg ggcagctcaa ccaccccgtg
     2701 gcagctcgag ggcgtcatcc gggccatgaa agccgacggc tacgacccgc agcgcatcca
     2761 cgcctgccat aatcgcactg tggtcatcga cgcccatctc ggcgaacgcg agaacaagca
     2821 gctcaacgtc gtcgaggccc atggcctgcg caacgtccac ctgtacgagg gcgaggagtg
     2881 gatcaacgtc cgtgacgcgg tcggcgacct cacccgccag ttcctgtgct tgaacgaggt
     2941 ctacccacgc ggcttcaaca tcccgcgccg cttcatcggc gagaacatca tccacctgcc
     3001 gaccactaag acgcacgtct tcaccaccat gaccggcgcc atgaagaacg ccttcggcgg
     3061 cctgctgaac gaacgccgcc actggaccca cccggtgatc cacgaaacgc tggtcgacct
     3121 gctgatgatc cagaaaaaaa tccatcgcgg cgttttcgcc gtcaccgacg gcaccttcgc
     3181 cggcgacggc cccggccccc gctgcatggt cccctacgta aagaacgtca tcctggccag
     3241 cgccgaccag gtcgccatcg acgcggtcgc ggccaaactc atgggcttcg accccctgtc
     3301 catcaaattc atccgcctgg cccacgaacg cggcctgggc tgcggcgatc cgcagaagat
     3361 cgaaatcgtc ggcgatcgcc aggcggccga ggagaactgg cacttcgccg gcccgttcca
     3421 gaagatgacc ttcgccagcc gcatgcaaca caaaatctac tggggtccgc tgaagggccc
     3481 gatcgagtgg tcgctgaaaa cggttctggc cccctgggcc tacatggcca gcgtgctcta
     3541 tcacgatagc ttttggtacc ctgtgcgggc caagaagttg atggccgacg tgctcagcag
     3601 cgattggggc cgcctgttcc gcaactggga aaacgcccgc cccgacgcca acggctaccc
     3661 tgatctcggc gtcgccccgg ccgagctgca ccgcaccggc tggcgcgcct tcgccaagtc
     3721 gatcggcatt ctcggtacgt gcatcaaaga agccccggaa ttcagcacgc gccgccggcc
     3781 ggtggccaaa tagaagccgt cgcgcttcca agcgccccgg ccggtcgtcc cacatccgac
     3841 tgattcgcgc gcctcgccgt cacgaagccg ctgtcagccg cggtttcgcc atcagcgcca
     3901 ggagctcacc gaagtatgac gccaacggcg gcagctccgg gaatttgtcc cccaactcct
     3961 ctcgcagacg cgcgttgtcg tatagcacgt tcatattcag gaacggcaga tagttccgca
     4021 gttgcccgaa tacgcggcgc tgctgcggcg tcgcaatgca gcgccggtgc agctcacgcg
     4081 tccattgcga agggtgtatt aattgcagtg ggcgggcgcg gccgtagaaa cggtctatca
     4141 ctgccgaaaa ctccgcataa caagccgagc cctgcgtacc ggccgaaatg tggtagcagt
     4201 cgtgcttgcg ccgcgggacc tgcagcaggc gaacgatgct ctgcacgacg aattgcaccg
     4261 gcaccgtatc ggctcgcgcg gccgggtcta tcggcaccgc ctcgaagacg gtcgaccatg
     4321 gaaggaagcc gagaatcgca cgcgcaaact gctcgtcggg caaacccgcg ctcagcacga
     4381 tcgacgggcg taccaccagc accggcaacc cgctcgaacg gaacaggtcc tcggccacag
     4441 ccttcgagcg ggtgtattcg ttgtggtgcg catccccaag cccgcttgtc tcggacagac
     4501 acacgttcgc aaccgccccg ctaacgtacg ccgtgctcaa gcataccagc agcggccgcc
     4561 gcctggcgct tcgcgcgaac tcgatcaagt tctgcgcccc ggcaatgttc gtctgccggc
     4621 acgcggccgc gcggataaac gacgtctcgg ccgcgctgtg aataatgatg tccgcgtcgc
     4681 gccgaatttc gtccaggtca tcggcggcga gccctaagtg cggcagacgc acgtcacccg
     4741 cgacggcgtg aacgccgagc gtcccgtcgc tcgaccccga gcggcgcagc cgctgcacga
     4801 ggcgcacagc cgggtcggcg tccgggcttg gccggatgag ggtccaaacc tttccaacgg
     4861 gctgctgcac cagcgcccgc accagttcgc cgccaatcag accggtagcc cccgtgacga
     4921 aaacgtttga tccggcgttc agcacgtcga cacgcgcatt catcgactcc tgaatacgta
     4981 agctggcctg tacgtatgct ggcctttgtt cacctgccca atcgtcgggc ggccaacgaa
     5041 ttcttgccgt aggcaacaac cgggccagcc gcccagacta tagcccaagc cggaacgcgc
     5101 cgctaaggca accggcataa tgcaaagagc gctaagaccg cctgcaacaa ggagttgcag
     5161 cgcgtccgag agaaaattcg cgccgccctg ccgttgcccg cggccgctaa ctgcctattt
     5221 atcggtactg gtaatctgcc caccacgtgt gatatgctgc gcagcaggtc cgcattgtat
     5281 cgggcggccg gtcgcgcaaa ccattccgcc cgcggcttca gcggcttttg ccgcaacgcg
     5341 accagtcctt gttccgacca ccgcgccgca gcacatcact cgcggcacga tcaaggaggc
     5401 cagacatgaa agctgctccg ctgacaattg cactgctctg catttgcctg ttgctgaccc
     5461 tcacgcccgg cggttgcccg ctacgtggcg gctcgaccaa ccagaacgat aacgccgccg
     5521 cgaacggcaa tctcaatagc gacgacgccg gccgcaacgc aaactccgcc gacggtaacg
     5581 tcaacggcgc cgccaacgcc aacgacaacg ccgccgtcaa tgccaacgac aacagcgccc
     5641 cgcctccgat cgcgcccggc ttctgggaga ttgtctcggg tgacctgctg cagaacgggc
     5701 cggttgacct cgcctacatg gcgctgaacg ccgaccacac cgttgatttc cattactaca
     5761 tgcccgagct gaacatcgcc gttggccaga ccggcattta cagcgtggcc ggttcggtct
     5821 tgttgctcgg cttcggcacc ccacccacgc cggtcatgct ggtgttcgac cagccggacg
     5881 cggacacgct gcacctgatg agcgatggcg gcgatgagct ggtgcttagc cgcgccgccg
     5941 agataccgga gcagttccaa tgcctgccgc tgacgccggt tcacacctac accggcctgc
     6001 cacggccgga cgagaggacc ggaatggcct atgacggcag ccggctctgg tacacgaccg
     6061 acgcctctgt gctgcaggcc attgatcctg cgcatgggac gccgggtgaa acgcatgttc
     6121 tgaccacgcc gctcgtgcac gcctatcagg gcggtgcgtt ctgggtggta tccggctcgg
     6181 gcgcgatgga acaggccgag cgccgccggc tcgacaacac gctgctggac gagatcgaca
     6241 cgcgggtcga cttgggcgag gccaaccgga tcgaggtcct ggcgtgggag tcgcgcgaca
     6301 acgtcctgtg ggtgcacggc tcgctgccgg atcacagcgc tgaccgcttc ctgcgcgtcg
     6361 cctgcctggg cgagccggac gagctgctgg gtattgacga gttcgatgtg gccatatcgg
     6421 ccatgagctg gcatggccgg gaactgtggg cgattgtgag cggcatggta gtgcaactcg
     6481 acatgacggc gcttaggggc gaggtgacgt accgcgtaga cctgccgggg atccgctggc
     6541 gaggtatcgc ctgggcgggc gacgcgatgt tcctgatcgg cgcgaacgac gacggcggcg
     6601 tgctagtgga ggtcgacccg gggtagcgga ggcgcggtac gctgggtgag cggtgcaaaa
     6661 atgaccccag tttcatgaac attgccactg cgtagtatca gatgatacca gcctgccttg
     6721 ccttcgtcat gatctcgtgc tatatttatg gtggaggatc gctaagaagg ctgccgaccc
     6781 agcgattgtg agactcatcc gcgagctggc cgccgatccg gatagagtcg cggtttaccg
     6841 gaccgcgaag tacgacttcc tggccccgac tttgacggtg gacgacgtct gcgacgccat
     6901 ttgtgaatgg atcgatcgcg gaaatccggt gattgaaacg gtcatccaca cgattcccga
     6961 gcggaagaac acgccggcat atgagctgaa accagtgctt tgtgaaaagc gctattacgt
     7021 gaaactggcc ctggaaagac agggcgaggg ctggctgctg atcttgtccg ctcacctcga
     7081 cgtgtaaata ggcgagcagg agaaacatat gggaagcgtc tgcctgacct gcggacaggg
     7141 cacaatggag ccgaagcgcg gcacgttccg cttcgatccg ccgcccaata tccctggcgg
     7201 cacaatcgag atcgaaaacg ccgaatggcg cgaatgcacg gaatgcggcg agcagatcct
     7261 cgggcgagaa ttggatcgcc gcctcgacga tgcggcgcgg caccggctgg gcctactatc
     7321 gccggccgaa atcaaggaaa tccgcgagcg cattgggctg agccaggagg ccatggccca
     7381 atggctaggc gtgggggaca agacgtacac gcgctgggag acgggcaagt cgatccaaaa
     7441 taaatcgaac gacacgttga tccggctcgt cgcgcgcaat gcgggtatct tctctgaatt
     7501 agacgcggag cgcgagcctg atcgctcaaa gagaatcgcc gagtacgtgc acagcttgca
     7561 cgatctcaaa ggcgaaagcc cgctggcgat ggcggcacac ggcggagagc tccccgagcg
     7621 cgtggtcgag gagttgcgac agcgcctgcg cgccattctg gaattccagc ggaaccgcaa
     7681 gggttgaccg tgatggagtt caaggccagc tacctggaaa tcgagccgcg cgaactgatc
     7741 gaattcctgc tgcgcgaaag tggtcacgct gatcgggagg cggtcaatcc aaagagcatc
     7801 cttgacgttt tgcggctgga gcatgtcacc gctaatttcg ccgcggagct gccgcccgag
     7861 ctcacgcaac gcagcacgcc ggtgcgggga atgttgtcgt accgcgaccg gatcgtggcc
     7921 actgatataa acctgaatgg cacgcgcgcc agattcagcg tgctgcacga ggtcgcgcat
     7981 tacgtcctac caacgcatca gcatgctctc tacgtgtgcg atgagacggg catgagcttt
     8041 cgcacgcgtg tcacaattga gcaggaggct aacgcatttg cggctgagct gctctttctt
     8101 ggcgaccgtt tcacgtccga agtgaatagc cggccactta ccgccgccac gatcaaactg
     8161 ctggcacaga agtatcaggc ctcctttgaa gctacagcgc ggcggatgat cgagcgaagc
     8221 ctgcggccgt gtatgctggt cgtgtgcgac aataccggca gtcgggtcga ccccgacgcg
     8281 caaccggtgt ggtcgaagaa gtacacgatc gcctccgtgc cattccggac gaagtacttc
     8341 agcgacctcc attccgccgt cattccgccc gacgtggccg cgcaggtgct cgctccgggc
     8401 cgggacattg cggacagtgt gacgcacgag gtgacgataa ggaccgcaac gggaacggat
     8461 gcccgcttta gggcggagtt cttttccaac acgtacagca tcttctgcct gctgaggccg
     8521 accgccaatt agcttagcct attgcacatt tgcgagatgg cgacctgccc cgaatccagc
     8581 aggaggacgg cacgcagggt ttgaaatggg gcgtcttggc gagcgctgcc ttgattggat
     8641 tagcgcggag cgaaaactcc gtggggggat ttacacgaac ggggggcgag acaggattaa
     8701 cgcaccgacg gggattttcc aagtagccgg attcggcgcc agccgcaatc gcgacaactt
     8761 cttctcccca cgcgaatatt ctcccgcacc cgctgcaact tcttgtcctt ggcggtcctg
     8821 gcgctctttg cgtcttggcg gttgcctttg gttgtggctg tggcggttgc cccgctgtcg
     8881 ccgcgcgcgg aacgcggtta cagtgccgcc ttgtgaccga accgggtcct gtacagcgag
     8941 aaggagtcgc ttatgaaccg cccgagaatt gccgcctggc tgaccacgac gctggtcgcg
     9001 ctcgtcgcgc tggcgccggc cgtcgcccaa ccgccgccgc caaccgacgg cgagcgcccg
     9061 ctcttc
//
LOCUS       DAOSYS010000148         9026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6558_length_9026_cov_5.579088, whole genome shotgun sequence.
ACCESSION   DAOSYS010000148 DAOSYS010000000
VERSION     DAOSYS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_9026_cov_5.579088"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1538)
                     /locus_tag="PLU99_09985"
     CDS             complement(216..1538)
                     /locus_tag="PLU99_09985"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOO17426.1"
                     /translation="MKRARNIQHAWILACGTELIIGHCADTNSAWLAEKLASVGIETD
                     RILTVPDRAALLANALREAAAESDVILLTGGLGPTEDDLTRTSLAEVAGVPLTVDPPS
                     LENIRAFFAERAREMPPANQVQAMIPRGARALPNLCGTAPGIAIELQGTPCFAMPGVP
                     FEMRAMFEREVLPELAALASSSVLLSRNLHCFGRGEADIGQSIRDLMAPGRNPIVGTR
                     ACLGVITLRIDARAADPPTAESMLDEAERELRARLGPIIYGRGDETLQSAVGELLVAA
                     GQTVAVAESCTGGLLGKLLTEVPGSSRYFLGGIISYADAVKTRLLGVPQETLRSHGAV
                     SEEVARAMAAGACAALASDWAISITGIAGPAGGSACKPVGLVYLGLKTPTEVQVSEFR
                     LGEHTPREVIRERAARCALNLLRVALLERTKADTPNPQQGSNHPAKPA"
     gene            1596..2663
                     /locus_tag="PLU99_09990"
     CDS             1596..2663
                     /locus_tag="PLU99_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17427.1"
                     /translation="MPNVSAFITSPRYRPVEELVVGNTVVAGRFGEVGQEYRAAREGA
                     ALFDRGDRGLLILTGADRTSWLQNLVTNDVAGLAENAGTYAFATDVKGRVVFDLNVLA
                     LRDALWLDIDRALIPRALAHLERFLISEDVRMRDASAEFSRLGWSGPGAGGIAEALGF
                     AAAADMPAQSSAALEGDGTRLVRHDFTGLPGYELIVPFAQAAAWWDRLVAAGAAPAGF
                     HVLDAMRIEAGIPWFGREIDEKVVAPEADPAGCGISYKKGCYLGQEVIERMRSRGVQA
                     RRLVRLRASPAVALDLPADLRKGGQTVGRITSLVRHPVEPVGVGLGYVGSSVCDLCGI
                     ECDASTELWVSEVDAHSVMCR"
     gene            2827..3966
                     /gene="ispG"
                     /locus_tag="PLU99_09995"
                     /gene_synonym="gcpE"
     CDS             2827..3966
                     /gene="ispG"
                     /locus_tag="PLU99_09995"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOO17428.1"
                     /translation="MLSRHKTRQVSVGRVKIGGDAPVALQTMTSGYTYDVDRCIAEIR
                     KLAQAGADLVRVAVPEKRDTEALPHILAASPVPIVADVHFHFERALEAIAAGVHKIRL
                     NPGNLQDRAKVNRVIAACKERGIPIRVGVNEGGIVERRDKERRAVEKATLAQDYTANL
                     IRLMLEKLEDYLRIFDDNDFHDVVISAKSIDPRVVIDVYTAISQRFDYPLHLGVTHAG
                     PPETGRIRSIAALGTLLANGIGDTIRISYAADPVHEVEDGKELLCALGLRRRSEPELI
                     ACPTCGRIEVDLFKLVTQVREKLATEIKLPMKVAVMGCVVNGPGECEGADVAIFAGKG
                     KGIIYVQGEQKQTVAEERMLDALLSECRDLETRVQRGEARLHGSA"
     gene            complement(4036..5022)
                     /gene="selD"
                     /locus_tag="PLU99_10000"
     CDS             complement(4036..5022)
                     /gene="selD"
                     /locus_tag="PLU99_10000"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOO17429.1"
                     /translation="MPQDGMAQVLRRLPPQKHPDLLVGTDTYDDAGVFRISDDVALVQ
                     TTDFFPPLVDDPFAFGRIAAANALSDIYAMGGVPLTALNLVGWPDQQLPYEVLAEILR
                     GGADACAEAGCIIVGGHSVRDREIKYGMSVTGKVHPKRIVTNANAKPGDKLVLTKPLG
                     TGVLCSAAKVGKLPQAELAEAIAVMSTLNKGACEAALAVGVNSMTDVTGFGLIGHAYE
                     MAEGSDVTLTIHAAEVPLMAHVLEMAEQGVITRAATTARKFLSTKLLIDNVDETLADV
                     LCDAQTSGGLLISVPAERCEALIAELKARGAVCATPVGEAKSRGEARIELVP"
     gene            5432..6310
                     /gene="rsmA"
                     /locus_tag="PLU99_10005"
     CDS             5432..6310
                     /gene="rsmA"
                     /locus_tag="PLU99_10005"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOO17430.1"
                     /translation="MAGQTQSEIRALLASVGLSPRHRYGQNFLIDLNLMRKVVEAADL
                     RPADTVLEVGCGTGSLTEMLLAAGAYVVGVEIDRGLQDLLRSRLGENPRFSLVAGDVL
                     AGKHRINPGVLAELGRRIPAAPGQYKLVANLPYQVATPLLMELLYIQPYFGRLTCTIQ
                     KEVGERLIAAPDTEAYGPISVIAATLADVRLVATFPPTAFWPRPKVESVLVDIKPLSD
                     PPIVAPDIPAFVEFVRGSFLHRRKTIKNVLQALIGRAAADAVLAGAGVNPQTRPEALG
                     PPVWRNLFERFRAACP"
     gene            complement(6228..7022)
                     /locus_tag="PLU99_10010"
     CDS             complement(6228..7022)
                     /locus_tag="PLU99_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17431.1"
                     /translation="MGRALSMLMLIAVSAFFATGCGPSFGPQAQKGITFYCPGAGNID
                     FGDGGIREGLSAAGYEGQVASVLWTVSFNPAVDQTLRLNARLAAARLARAIETYQDQY
                     PGRPVNVIGLSAGTGVAIWAVESLSKGHSVDNVILLSSSLSSDYDLNKALPNVKGKIY
                     NYYSSEDSILAGPMKLFGTIDGRYFVDGAGAVGLHPPSRADRVVNIGWRQEFARYGYY
                     GGHTDVTSPAFVRAEISGRVTGMPLETAQTGSARPVAQALRAASAD"
     gene            complement(7118..7519)
                     /locus_tag="PLU99_10015"
     CDS             complement(7118..7519)
                     /locus_tag="PLU99_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17432.1"
                     /translation="MFNFDWFHGGGLQRQVEEAKNRCAYKKFPQAKNNSNGSPEFHAY
                     RAQLETRTILYLKDGLSYEMREMADMGISSALSFECVPVDENYQVGAYVVVVPFEDIA
                     RVEVFAVHPDDKPQDNIRIPGFKSAAESSRD"
     gene            7621..8688
                     /gene="queG"
                     /locus_tag="PLU99_10020"
     CDS             7621..8688
                     /gene="queG"
                     /locus_tag="PLU99_10020"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOO17433.1"
                     /translation="MTKSLATALGFERVGITQAGPSERGAYYKAWLEAGYAGTQEYLK
                     RNVGLRSDPSQLLSGARSIICVAVSYNRAETAPETGGEGEHPRGRVASYARGRDYHVV
                     LRRMLTELMERLRAELNEAFEWRICVDTAPLLERDLARQAGLGWIGKNTCLLDRQVGS
                     YLFLGEALTTLDIEPDSPVTDHCGTCTRCIDACPTGALIAPYQLDARRCISYLTIEHR
                     GEISEEFHAAMGNCVYGCDICQQVCPYNQHARPARQPEILTEHTPAMPDLLELVRMRS
                     GEYRRLTDGSASRRATRNMWRRNAAIALGNVRCADEALRHTIGVALSEAAEDDYLPLR
                     AAAVKSLARLGADRGRFRKKD"
     gene            complement(8715..>9026)
                     /locus_tag="PLU99_10025"
     CDS             complement(8715..>9026)
                     /locus_tag="PLU99_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17434.1"
                     /translation="QPDVLTARTEFALLEKQIEFEPPTANLPLLRSAAEATASAGGQY
                     CPLQDFPEMLDKLSRTDRRQEVRRPMRYALLEQHAWPALLTAAAVLGLEWLIRKRLGL
                     P"
BASE COUNT         1545 a         2759 c         3035 g         1687 t
ORIGIN      
        1 gggctgcgaa gccgcgggtg cctgggccgg gtcttgcgcg tagcccgagc aagcgaacag
       61 ggccaatccg agaactgcgg ccgtcaatcg aaacattcat tgctcctcac aacggtcgca
      121 aaggcggccg tcgcttgtga agattgggcg gtcgggaacc cgccctcatt atcgggcacc
      181 tccgcggggc gcgtaaagtg gctggggcac atagattacg ccggcttcgc ggggtggttc
      241 gagccctgct gagggttcgg agtatcggct ttcgttcgct ccagcaaagc cacgcgcaac
      301 aggttcagcg cgcatcgcgc ggcgcgctcg cgaattactt cgcgtggcgt gtgctcgccc
      361 aggcgaaact cgctgacctg gacctccgtg ggcgttttca agccgaggta cacgagcccc
      421 accggcttgc aggcagaccc gccggccgga ccggcgatgc cggtgattga gatcgcccaa
      481 tcgctggcca gtgcggcgca ggcccctgcg gccatggccc gagctacctc ttcgctgacc
      541 gcgccgtgcg agcgcagtgt ttcctgcgga actcccagca ggcgcgtttt gacggcgtcg
      601 gcataactga tgatccctcc caagaagtag cggctgctgc ccgggacttc cgtaagcagc
      661 ttgcccaaca gtccgccggt acaggattcg gcgacggcaa cggtctggcc ggccgcgaca
      721 agtagttcgc caacggcgga ttgcagcgtc tcgtcgccgc gtccgtagat gatcgggccc
      781 agccgtgcgc ggagttctcg ctccgcctcg tccagcatac tttccgccgt gggcgggtcg
      841 gcggcgcggg catcgatgcg cagcgtaatc acgccgaggc aggcgcgggt gccgacgatc
      901 gggttgcggc cgggggccat caggtcgcgg atgctctggc cgatgtcggc ttcgccgcgc
      961 ccgaagcaat gcaagtttcg gctgagcagc acgctgcttg aggcaagggc ggcgagttcg
     1021 ggcaacactt cgcgctcgaa catggcccgc atctcaaacg gcacaccggg catggcgaaa
     1081 cacggtgtgc cctgcagctc tatggcgatg cccggcgcgg ttccgcagag attgggcagg
     1141 gcacgtgcgc cgcgcgggat catggcttgc acctggttgg cgggaggcat ttcacgggcg
     1201 cgctcagcga agaatgcgcg gatattctcg aggctgggcg gatcgacggt caggggcacg
     1261 cccgcgactt cggccagcga cgtacgggtg aggtcatctt cggtcggacc gaggccgccg
     1321 gttagcagga ttacgtcgct ttctgcggca gcttcgcgta gggcatttgc caggagggcg
     1381 gctctatcgg gcaccgtcaa gatgcggtcc gtctcaattc ccacgctcgc cagcttctcg
     1441 gcgagccagg cggagttggt gtcggcgcaa tggccgatta tcagttcggt tccgcaggcg
     1501 agaatccagg cgtgttggat gttgcgggcg cgtttcattg cattacgttc ccgggcaggc
     1561 gtaagagggc gctacgatac caaggaggcc gatgtatgcc aaacgtttct gcgttcatca
     1621 cgagtccgcg ttatcgaccg gttgaagaac tggtcgtggg caacacggtc gtggcgggcc
     1681 ggttcggaga ggtggggcag gagtatcgcg cggcgcggga aggcgcggcg ttgttcgacc
     1741 gcggcgaccg tggtttgttg attctgacgg gcgcggaccg cacgagctgg cttcagaacc
     1801 tggtcactaa cgatgtggcg ggactcgcgg agaatgcggg cacgtacgcg ttcgcaacag
     1861 acgtgaaagg ccgcgtggtc ttcgatctca atgttctcgc gcttcgggac gcgctctggc
     1921 tggatattga tcgagccttg atcccgcgcg ccctggcgca cctggaacgc tttttgatca
     1981 gcgaagatgt gcgcatgcgc gacgcctccg cggaattctc ccggctggga tggtcgggcc
     2041 cgggcgcggg cggaatagcg gaggcgctgg gctttgcggc tgcggccgat atgcccgccc
     2101 agtcgagcgc ggcgcttgag ggcgacggca cgcgcctggt tcgccatgac ttcaccggcc
     2161 tgccgggata cgaactaatc gtcccgttcg cgcaagccgc ggcctggtgg gaccggttgg
     2221 ttgcggccgg cgccgcgccg gcggggtttc acgtattgga cgcaatgcgg attgaagccg
     2281 gcattccctg gtttgggcgg gaaatcgacg agaaggtggt ggcgccggag gccgatccgg
     2341 ccggttgcgg gatcagctac aaaaagggct gctatctggg gcaggaggta atcgagcgga
     2401 tgcggtcgcg tggcgtccag gcaaggcgac tggttcggct gcgggcttcg ccggcggtcg
     2461 cactcgattt gccggctgac cttcgcaagg gtgggcagac ggtgggccgg atcacgtcac
     2521 tggtgcgaca cccagtcgag ccggttggag tcgggttggg ttacgtgggg tcgtctgtgt
     2581 gtgatctgtg cgggattgag tgtgacgcga gcacggagct gtgggtttct gaggttgatg
     2641 cgcactcagt catgtgccgc tgacggaatt catgccggcg cggtagtatt cttgatgcag
     2701 tcgccgtttc agccacgtat attgcggacc gcacgtggcc gcgggcgagc gcgagtagtt
     2761 ttaaatgtgc tacgatagac gttctggatt cgtctgctgc ggcagagcac ctgatttgga
     2821 agaatgatgc tctcacgcca caaaactcgt caagtgagtg tcggccgtgt caagatcggc
     2881 ggcgacgcgc cggtggcgct gcagacgatg acttccggct acacatacga cgtcgaccgg
     2941 tgcatcgccg agattcgcaa gcttgcacag gccggggcgg acctggtgcg cgtggctgtg
     3001 ccggagaaac gcgacacgga ggcgctgccg cacattctcg cggccagtcc ggtgccgatt
     3061 gttgcagacg tacactttca ctttgagcgg gcgttggagg ccatcgccgc gggcgtgcac
     3121 aagatccgcc tcaacccagg caacctgcag gatcgggcca aggtcaaccg cgtcatcgcg
     3181 gcgtgcaagg aacgcggcat tcccatccgc gtcggagtca acgagggcgg aatcgtcgag
     3241 cgccgcgaca aggaacgccg cgccgtggag aaggccacac tggcccagga ctacacggcc
     3301 aacctgatcc gcctcatgct ggagaagctc gaggattacc tgcgaatctt cgacgacaac
     3361 gattttcacg acgtggtgat ctctgcgaag agcatcgacc cgcgcgtggt gatcgacgta
     3421 tataccgcga tcagccagcg cttcgactac ccgctgcacc tcggtgttac gcacgccggg
     3481 ccgcccgaga ccggccgcat ccgctccatc gccgcgctgg gaacgctgct ggccaatggc
     3541 atcggcgaca cgatccgcat cagctacgcg gccgacccgg tgcacgaagt ggaagacggc
     3601 aaggagctgc tctgcgcgct tggtctgcgg cggcggagcg agcctgagct gatcgcctgc
     3661 ccgacctgcg gccgcatcga ggttgatcta ttcaagctcg tcacgcaggt gcgggagaag
     3721 ctggcgactg aaatcaagct gcccatgaag gtggcggtga tggggtgcgt ggtgaacggt
     3781 ccgggcgaat gcgaaggggc ggacgtggcc attttcgccg gcaagggcaa gggcatcatc
     3841 tacgtgcagg gcgagcagaa gcagaccgtg gccgaagagc gcatgcttga tgcgctgctt
     3901 tccgaatgcc gcgatttgga aacgcgggtg cagcgcggcg aggcgcggct gcatggttcg
     3961 gcgtgagggc ggcgctcgca ggtttgggcc gcgccgtttc gtcaaaggcc tgatcggcag
     4021 tacacggcta agtgattagg gcactagctc gatcctcgct tcgccccgcg attttgcttc
     4081 accaaccggc gtagcacaga cggcgccgcg cgccttcagc tcggcgatca gagcttcaca
     4141 gcgctcggcc ggcacactga tcagcagacc gcccgaagtc tgtgcatcgc agagcacatc
     4201 cgcgagcgtc tcatcgacgt tgtcgatcag cagcttcgtg ctgaggaatt tccgggcggt
     4261 cgtggcggct cgcgtaatta ctccctgctc ggccatttcc aggacatgcg ccatgagcgg
     4321 gacttcggcc gcgtgaatgg ttagcgtgac gtcgctgccc tcggccattt cgtacgcatg
     4381 cccgatcagt ccgaagccgg tgacgtcggt cattgaattg acgcctaccg ccagcgccgc
     4441 ttcgcaggcg cctttgttca gcgtgctcat caccgcgatc gcttcagcca actccgcctg
     4501 cggcagtttt ccgactttgg ccgcgctgca cagcacgccg gtacccagcg gcttggtcag
     4561 aacgagcttg tcgcccggct tggcgttggc gtttgtgacg atgcgcttgg ggtgaacttt
     4621 acccgtgacc gacatcccgt acttgatctc gcgatcgcgc acgctgtgtc cgccgacgat
     4681 gatgcagccc gcctcggcgc aggcgtctgc cccgccgcgc agaatttccg ccagaacctc
     4741 gtacggaagc tgctggtccg gccagccgac cagattcagg gccgtgagcg gcacgccgcc
     4801 catggcgtag atgtcgctga gcgcattggc ggccgcgatt cgcccgaagg cgaatggatc
     4861 atccaccagt ggcgggaaga aatcggtcgt ctgcacaagc gctacgtcat cgctgatgcg
     4921 aaagacgccg gcgtcgtcgt acgtatccgt cccgaccagc aggtctggat gcttctgcgg
     4981 gggtaagcgg cgcaggacct gcgccatgcc gtcttgcggc agcttgcccg ctcagccggc
     5041 gcagaaggcg taatctgtca ggcggatgtt ccgcaggtct gtcatgcgca ctcccaagaa
     5101 ccagactatt gactaaacca acgaccaaag cgcaaccggg ctctttgccc cggggagcgc
     5161 ctgggccgcc ggggcgcctg ggtgccatgc ccaagccgcg tggcggcgcg ggcatgcgcc
     5221 tgccggttgc ccggccttgt tcggtgtcgc cttacccagg gcatgcccgc gcttacagct
     5281 tgggcatggc acccgcgctt accggagggc atgcccgcgc ttacagcttg ggcatggcac
     5341 ccgtcctatg gcacccgtct ttgggcaggg cacccagcag cacccacttt ctgcttgccg
     5401 taatgctatc agccggctta cataggtcca aatggccggt cagactcaat ccgaaattcg
     5461 cgctctgcta gcctccgtcg gcctgtcgcc gcggcaccgc tatggccaga actttctcat
     5521 cgacctgaat cttatgcgca aggtggtcga ggcggccgac ctgcggcctg cggacaccgt
     5581 gctcgaggtc ggctgcggta ccggctcgct gaccgaaatg ttacttgcgg ccggcgcgta
     5641 cgtcgtcgga gtcgagattg accgcggact tcaagacctg ctgcgcagcc ggctcggcga
     5701 gaacccgcgt ttttcgctcg tggccggcga cgtcctggcc ggcaagcacc ggatcaaccc
     5761 cggcgtcctc gctgaattgg gccgccgcat tcccgcggcg cccgggcaat acaaacttgt
     5821 cgctaacctg ccgtaccagg ttgccacgcc gctgctgatg gaactgcttt acatacagcc
     5881 ctacttcggc cggctgacgt gcactattca aaaggaagtg ggcgaacgcc tgatcgccgc
     5941 gcctgacaca gaggcgtacg ggccgatctc cgtaattgcg gccacgctgg ccgacgtgcg
     6001 gctcgtggcc acctttccgc ccacggcgtt ttggccgcgt cccaaggtcg aatcggtgct
     6061 ggtcgacatt aagccattga gtgacccacc cattgtggcg ccggacattc cggcgtttgt
     6121 cgagttcgtg cgcggcagct ttctacatcg ccgcaagact atcaagaacg tgctgcaggc
     6181 tttgatcggc cgggcggcgg cggacgcggt gctcgcgggc gcgggcgtca atccgcagac
     6241 gcggcccgaa gcgcttgggc caccggtctg gcggaacctg tttgagcggt ttcgagcggc
     6301 atgcccgtga cgcgccccga gatttcagcc cgcacgaagg ccggactggt tacgtcggtg
     6361 tggccgccgt aatagccgta gcgggcgaac tcctgtcgcc agccgatgtt cacgacgcgg
     6421 tctgccctgc tgggcgggtg cagtccgacg gcgccggcgc cgtctacgaa gtaccggccg
     6481 tcgatggtgc caaagagctt catcggacct gcgaggattg agtcctcgga cgagtagtag
     6541 ttgtatatct tgcctttgac gttcggcagg gctttgttca ggtcataatc ggagctcaag
     6601 cttgaactga gcaggatcac gttgtcgacg ctgtgccctt ttgacaagct ctcgacggcc
     6661 cagatggcga cgcccgtgcc ggcggaaagt ccgatgacgt tcaccggccg gcccggatac
     6721 tgatcttgat acgtctcgat agcgcgcgcc aggcgtgcgg ccgcgagacg cgcgttcagg
     6781 cgcaatgtct gatcaacggc gggattgaac gagacggtcc atagcacgga ggcgacttgc
     6841 ccctcgtatc cagcggccga gaggccctcg cgtatgccgc cgtcgccgaa atcgatgttg
     6901 cccgcgccgg gacagtagaa tgtaattcct ttttgagcct gggggccaaa ggacggtccg
     6961 cagccggtcg cgaagaacgc ggatacggcg atcagcatca gcatgcttaa ggcgcgtccc
     7021 atcgatggac cctggttaca gctacaggcc gcgcgtcgcg gcacgtaagc cgtgcgtcta
     7081 aaaagacagt ccactggcgc gaacgttagc ggcgcttcta gtcccggctc gactccgcgg
     7141 ccgacttaaa gcccgggata cgaatgttgt cctggggctt gtcgtccggg tgaaccgcga
     7201 atacctccac gcgtgcgata tcctcgaacg gaactaccac gacataagcg ccgacctggt
     7261 agttctcgtc gaccggcacg cattcgaagg atagcgcgct actgatcccc atgtcggcca
     7321 tttcgcgcat ttcatagcta agcccgtcct tcaagtagag gatcgttcgc gtttcaagct
     7381 gtgcgcgata ggcgtggaat tccggcgagc cgttgctgtt gttcttggct tgcggaaact
     7441 ttttgtaagc acagcggttc ttggcctctt cgacctggcg ttgcaagccg ccgccatgga
     7501 accagtcgaa attaaacatg aacgaacctt tcatcccgga agggcacacg ggcatcgctt
     7561 ggtccgatgt tcggcacgca gctatcatcg tcgcgtatga cgccgtccaa cagagcccgt
     7621 ttgaccaaat ccctcgcaac ggcgctgggc ttcgagcgcg tgggcatcac tcaggccggt
     7681 ccgagcgaac gcggggcgta ttataaggct tggctggaag cgggctatgc cgggacgcag
     7741 gagtatctca agcgaaatgt cggattgcgt agcgatccgt cccagttgct gagcggggcg
     7801 aggtcgataa tctgcgttgc cgtctcatac aacagggccg aaaccgctcc cgaaactggt
     7861 ggggagggcg agcatccgcg tggccgcgtc gcttcatacg cgcggggccg cgactaccac
     7921 gtggtgctgc gccgcatgtt aaccgagctg atggaacgct tgcgcgcgga gctgaacgag
     7981 gcgttcgagt ggcggatttg cgtcgacacg gctccgcttt tggaacgcga tctggcccgg
     8041 caagctggct tgggctggat tggaaagaac acctgcctgt tggatcggca ggtcggttcg
     8101 tatctgttcc tgggcgaggc gctcacgacg ctggacatcg agccggacag cccggtcacg
     8161 gatcactgcg ggacgtgcac gcgctgcata gacgcctgcc caaccggggc tctcatcgcc
     8221 ccgtaccagt tggacgccag gcgttgcatc agctatctca cgattgagca ccgcggtgag
     8281 atttctgaag aattccacgc cgcgatggga aactgcgttt acggctgcga catatgccag
     8341 caggtttgcc cgtacaacca acacgcccgg cccgcgcgcc agcccgagat actgaccgaa
     8401 cacacgcccg cgatgccgga tttgctcgag ttggtgcgca tgcgctccgg tgagtaccgg
     8461 cgattgaccg acggctctgc cagcaggcgc gcaactcgca acatgtggcg ccgcaatgcc
     8521 gcgatcgcac tgggaaatgt gcgctgcgcc gatgaggccc tgcgccacac gatcggggtc
     8581 gcgctgagcg aggctgccga ggacgattat ctcccgctgc gcgcggccgc cgtgaaatcg
     8641 ctggcgcggc taggggcgga tcggggtcga tttcgcaaga aggattgaac gccgacgctt
     8701 ggtttcgacc ctgcttacgg gaggccgagg cgtttgcgga tcaaccattc caggccgagc
     8761 acggcggcgg cggtcaggag ggcgggccag gcgtgctgct ccaggagggc gtagcgcatt
     8821 gggcggcgaa cttcctggcg gcggtcggtt cgcgatagtt tatctagcat ttccggaaaa
     8881 tcctggaggg ggcaatactg gccgccggcg ctggccgtcg cttccgcggc tgagcgcagc
     8941 agcggcaggt tggcggtggg gggttcgaat tcgatttgct tttccagcag ggcgaattcg
     9001 gtgcgggccg tcagcacgtc cggctg
//
LOCUS       DAOSYS010000149         9005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6578_length_9005_cov_5.189050, whole genome shotgun sequence.
ACCESSION   DAOSYS010000149 DAOSYS010000000
VERSION     DAOSYS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6578_length_9005_cov_5.189050"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..716)
                     /locus_tag="PLU99_10030"
     CDS             complement(159..716)
                     /locus_tag="PLU99_10030"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOO17435.1"
                     /translation="MDFGVLATCLLIIVARIGDVSLGTMRTIMVVQGRRWISWGLGFF
                     EVLIWLYAVSSVMRNLEHPIYALCYAFGFATGNYVGLTVERWLSLGDQAVRIFTRKGP
                     AMAGFLRENQFGVTEFDGRGRDGPVHHLFIQARRRGIARLVNLARQIDPECFYVVEDV
                     RSASTAYANGSSREPTGWRAIIKKK"
     gene            complement(1056..1424)
                     /locus_tag="PLU99_10035"
     CDS             complement(1056..1424)
                     /locus_tag="PLU99_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17436.1"
                     /translation="MCDVPVRWILGFCTCTWSAARAFGEDLANAKMTFWGDPTAVERM
                     SRAFFQGQAQGMWVGGLENTLPEDVKTAAKSGVSALGATVAAAVKKVLGIEIDPAAAE
                     AALRESVNGPQAAASGAAGA"
     gene            complement(1717..2154)
                     /locus_tag="PLU99_10040"
     CDS             complement(1717..2154)
                     /locus_tag="PLU99_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO17437.1"
                     /translation="MEIYTIGFTQKSAREFFGALSAAGIRRLIDVRLNNSSQLAGFTK
                     KDDLIFFLDRICGAEYLHEPQLAPSPEILEGIKKKKISWDEYERQFLALLKQRRIAKT
                     LDPAIFSVPAVLLCSEPTAEHCHRRLVAEYLQRNWPDVSITHL"
     gene            complement(2094..2669)
                     /locus_tag="PLU99_10045"
     CDS             complement(2094..2669)
                     /locus_tag="PLU99_10045"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOO17438.1"
                     /translation="MVIYTIGHSNHTIEDFIGLLRKSGVQVLVDVRSKPVSGYCPHFD
                     KEPLTRALRASGVQYLFLGAELGGRPEETEFYDAAGYVLYDKIARSARFQAGIERLLE
                     AAGPRLTAIMCGEDDPATCHRHLLIARVLAEGGHTVIHIRGDGSRVSYEDLLNRARSA
                     QLELFDYMEPAPWKSTRSVLRRSQPESSSEP"
     gene            complement(2818..3432)
                     /locus_tag="PLU99_10050"
     CDS             complement(2818..3432)
                     /locus_tag="PLU99_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOO17439.1"
                     /translation="MHAVRHYHERVREFMCEKVQPGGIVLLGSSHLEWFDTNHYLPGR
                     RFVNRGIAGDRLGIGKTGILHRLDVSVFNCEPSFIVFENGANDLGELWENGRPPMEAI
                     MAAYERVVATIRGRLPQTPMLIVNVLPTRSRYTGLNPYIREFNPHVRQVAEKYACMHM
                     DFYSEVVDAAGELRRELAEDGLHLNDAGYTLWAARLDELLPPTG"
     gene            complement(3452..5065)
                     /locus_tag="PLU99_10055"
     CDS             complement(3452..5065)
                     /locus_tag="PLU99_10055"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOO17440.1"
                     /translation="MEVEHGIVGLRDWTLARAVEPAVRVGIFLDGDAAAAVHLTPVRG
                     PAVLGSPGGPDVVIDPGTEIEVRHARPALSLRISGQAARTVPSLRLHAAPVADDLRVS
                     KPGDCEAGILLRDVPAGRGFHWEKRVSQILPGTLELSVGRHGVVLVNVLPLETYLAGV
                     ITAEMSAACPLEFLKAQAIVARSWLLAMTEPKHRGEPFDRCNDDCCQRYQGTGGLTAT
                     ALAAVHDTRGVVLLSPAERRDGSEPEAGGRADQSEPEAPARAKDEGIEGSRDQEACSR
                     PEARAPSEPEARGRANGARRGDTHRSCSILDANYSKCCGGVTETPWAVWGLDKPGIRP
                     VVDAPPDAPDRRFFPVSDANLEEYLDGAWLARTQVYCSPNVVAPDSLSRYLGRVDAVG
                     DYFRWAVRYTRAELEELLRVNLTDAAGLTELRDIHVRARGASGRAYAVQIDWLDAQGQ
                     PVRSRLDGEYRIRQALHRTFLYSSAFAVRPERDPGGRLSAIVLRGAGWGHGVGMCQIG
                     ALGMALSGQSSEAICSHYYAAAKLGGVYT"
     gene            5212..5478
                     /locus_tag="PLU99_10060"
     CDS             5212..5478
                     /locus_tag="PLU99_10060"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HOO17441.1"
                     /translation="MGIVVLFYSNEHEPVHVHGKCRGRESRAELILREGRVVDIIYTL
                     VRDRQPLAGVSFRTFKRSSSITQMKSFRNGSITSCSTTVCRRRC"
     gene            5492..5752
                     /locus_tag="PLU99_10065"
     CDS             5492..5752
                     /locus_tag="PLU99_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOO17442.1"
                     /translation="MSIGESAPIFVERAEHAGEFRIRLAFNDGTVRTIDFEPFLRRSR
                     NPLIQAYLDPGLFSAFSIKEGDLVWGDYDLCFPIADLYDGRL"
     gene            complement(5988..8864)
                     /locus_tag="PLU99_10070"
     CDS             complement(5988..8864)
                     /locus_tag="PLU99_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOO17443.1"
                     /translation="MKKFVYFFGAGKAEGKGTDKNLLGGKGAGLAEMTNIGLPVPAGF
                     TIAVDACAYFHEHNGTWPEGLEKEVRQQLARLEKTVGKKLGDKNNPLLVSVRSGAARS
                     MPGMMETILNLGMNDQSVEGLARNTKNPRFAWDSYRRFLQMYSTTAVGLSKDILEEKL
                     HNLKERLDKRYDYELPAEALQELCGQFKDFFREQTGREFPQDPWEQLVGAINAVFNSW
                     NAEKAVTYRRVEKITDLTGTGVNVQQMVFGNMGEDSGTGVCFTRDPSTGANEFYGDML
                     INAQGEDVVAGIRTPLHMSELAERMPAMYDQLCAVRAMLEVHYGEMQDVEFTFERGKL
                     YILQCRTGKRTPHAAFRIAVEQATQPLMRREEARRLAKAGYLPRKYEERAARPVISQD
                     DAIARITGNDIERLFYPIISQRVPREELGRRRIAEGINAVPGAACGKVVFTAREAEAL
                     AEQGEDVILVRKETSPEDVGGMQAAKGILTQTGGKTSHAAVVARGWGKCCIVGCEALR
                     IDYGTKQMSVNGKVVRQGEFLTLDGSIGAVYEGKLDLERPEPPQEYYTLMGWADQRRE
                     IGVRTNADTPEDARKAIQMGAEGIGLCRTEHMFFATHERQLAIQEMILADTEAKRRAA
                     LDKLLPFQRSDFIGIFEAMEGNPVTIRLIDPPLHEFVPKTREEAERLSAVTGVNPNDI
                     LARAEQLHESNPMLGHRGCRLCITYPEILEMQVRAIIEAAVDLKKRGLPVNPEIMIPL
                     TIDPKELRILADRTRAVADGVIAEAGIELSYLVGTMVETPRAALLAGPVAETAEFFSF
                     GTNDLTQMTMGLSRDDAGRFLPEYIKTGIFKADPFQSLDQEGVGQLVRMAVENGRRAR
                     GKLKIGICGEHGGDPESVKFCHRVGMNYVSCSPYRVPIARLAAAQAAIESGPSPLKGK
                     APRGTSKKAASAAKRPAKPAAKSKPAARAKAGARKAKAAKKSRK"
BASE COUNT         1601 a         3103 c         2667 g         1634 t
ORIGIN      
        1 aacaacagga ggctgagcgc gtcgcggccg attacctcgc gggcatgacc gaccgctact
       61 gcctcgaggt tcacgcccag gtgaccggcc gcaaagccgg gccaacagcc aacgctactt
      121 tttcttaaag attacccaac accgccccgc cgacgacgct acttcttctt aatgatcgcc
      181 cgccagcccg tgggctcacg cgatgagccg ttggcgtagg cggtcgaagc gctgcgcacg
      241 tcctcaacca cgtagaaaca ttccggatca atctggcgcg ccagattaac cagtcgcgct
      301 atgccgcgcc gccgtgcttg tatgaacagg tggtgcacgg gaccgtcgcg cccgcggccg
      361 tcaaactcgg tcacaccgaa ctggttttcg cgcaagaagc cggccatggc aggtccctta
      421 cgggtgaaaa ttcgcaccgc ttgatcgcct agcgacagcc agcgttcgac cgttaagccc
      481 acgtaattgc cggtcgcgaa accgaaggcg taacacagcg cgtagatggg gtgctcgagg
      541 ttccgcatga cgctcgaaac cgcatacagc cagatgagca cctcgaaaaa gcccaggccc
      601 cacgaaatcc accgccggcc ctgcacgacc atgatggtcc gcatcgtgcc cagcgaaacg
      661 tcgccgatgc gcgcaacgat gatcagcagg cacgtagcca gcacgccgaa atccatgtcc
      721 gttccctcgg cagtctagtg gccccatcgc ccacccccgg ccaagccccg cattatcgcc
      781 accccaagcc cggccggcaa ccccgcgtta accgcgtcaa tgttgtcaaa gttgggcgtc
      841 gacgttggct accaacgttg ggtgcccacg ttgggcgccc gcgttgggcg cccgcgttgg
      901 gtgccatgcc caagctctaa gcgcgggcat gccagccgta agaatcgtgc gcccattcca
      961 gccgggcgaa ccatgcgact gtcgtgcccg agcagcaagc gcgggtgcca gacgggagcc
     1021 cgctgctcta ttacgcaccc gcaagcgctg ttctctcacg cacctgccgc gcccgatgcg
     1081 gcggcttgcg gaccgttcac ggattcccgc agtgcggcct cggcggcggc cgggtcaatc
     1141 tcgatgccca gcactttctt cacggccgcc gcgacggtcg cgcccaaggc ggaaacgccc
     1201 gacttggcag ccgtcttcac gtcctcaggc agcgtgtttt ccagtccgcc cacccacatg
     1261 ccctgggcct gcccctggaa gaaggcgcgg gacatgcgct ccacggcggt ggggtcgccc
     1321 cagaaggtca tcttggcgtt ggccaggtcc tcgccgaacg cccgcgccgc gctccaagta
     1381 catgtacaga atcccaatat ccaccggacc ggcacgtcgc acatgctccc agcgattgcg
     1441 cgccaacagc caattttcgg gttgataagc ctccgggcgt ggctcgaccg cccacattct
     1501 aatgacgtcc agcggcagaa cctccctgcc gccgcaacag gcttgctcga cgcgcaacgt
     1561 gccgtgctcc gcgtttgtga cgggccgaaa ccacccgccg ccatccaacc gcagcccggc
     1621 cacacagcgc ccaaggtact tgcgcgagtt cgccagacaa atgatttcaa aggctctggc
     1681 ggccacgata caccgtcaac tatttgccac gcacccctac aggtgcgtga tggacacatc
     1741 cggccagttc cgctgcaaat actcagccac caggcgccgg tggcagtgtt cggccgtcgg
     1801 ctcgctgcac agcagcacgg cgggcacgct gaaaatggcg ggatcaagcg tctttgcaat
     1861 ccggcgctgt ttcagtagtg cgaggaactg ccgttcgtac tcgtcccagg aaattttctt
     1921 cttcttgata ccttccagga tttccgggct cggcgccagt tgaggttcgt gtaggtactc
     1981 cgccccgcag atgcggtcca ggaagaagat cagatcgtcc ttcttcgtga acccggcgag
     2041 ctgggacgag ttgttcaggc gcacgtctat caggcgccga attcccgcag cgctcagggc
     2101 tccgaagaac tctcgggctg acttctgcgt aaaaccgatc gtgtagattt ccacggggca
     2161 ggctccatgt aatcaaacag ctcgagttgc gcggagcgcg cacggttcag caagtcttca
     2221 taagatacgc gcgagccgtc accacgtatg tgaatcaccg tgtgaccgcc ctcagccagc
     2281 acgcgtgcga tcagcagatg gcggtggcag gtggccgggt cgtcttcgcc gcacatgatc
     2341 gccgtcaagc gcggcccggc cgcctccaga agacgctcaa tcccggcctg gaaccgagcc
     2401 gaacgggcga tcttgtcata caagacgtag ccggctgcat cgtagaattc cgtttcttcc
     2461 ggccgcccgc ccaactcagc gccgaggaat aaatactgga cacccgatgc ccgcaaagcc
     2521 cgcgtcagag gctccttgtc gaaatgcgga cagtagcccg ataccggctt cgaacgcacg
     2581 tccaccagca cctgcacccc cgacttgcgc agcagaccga tgaagtcctc gatggtgtga
     2641 ttcgaatgtc caatagtata tatgaccact tcggtccccg ttccggacaa aagtcatgcc
     2701 catgatagcc ccgccccagc cgcactccaa gaccttattt ggcgcgccgc ggttgccttg
     2761 cccttcgcac taccgcgctg caacgacgcc ggcacgtccc ccggccttcg cccattctca
     2821 cccggtgggt ggaagcagct catccagccg cgccgcccac agggtgtagc cggcgtcgtt
     2881 gaggtgtaac ccgtcctcgg ccagttcgcg ccgcagctca cccgcggcgt ctaccacttc
     2941 actgtaaaaa tccatgtgca tacaggcgta cttttcggcc acttgccgca cgtgtgggtt
     3001 aaactcgcga atgtacgggt tcaggccggt gtaacggctg cgcgtcggaa ggacgttgac
     3061 aatgagcata ggcgtctgcg gcaagcgccc gcggatggtc gccacgacgc gctcgtacgc
     3121 ggccatgatg gcttccatcg gcggccggcc gttttcccat agctccccca agtcattcgc
     3181 gccattctca aagacgataa agctcggctc gcagttgaaa acagacacgt ccaaccgatg
     3241 caaaataccg gtcttgccga tgcccaggcg atcgccggca atgccgcggt tcacgaagcg
     3301 gcgccccggc aagtagtgat tggtgtcaaa ccattccagg tgcgacgagc ccaacagcac
     3361 aatccccccc ggctggacct tctcgcacat gaactcccgg acgcgctcgt gatagtgcct
     3421 gactgcgtgc attgcttcgc cgaaactcca atcaggtata cacgccgccc agctttgcgg
     3481 ccgcgtagta atgactacag attgcttccg agctctggcc ggacaacgcc atccccaatg
     3541 ccccgatctg gcacattcca accccgtgcc cccagcccgc gccgcgcagc acaatcgcgc
     3601 tgagccgccc cccggggtcg cgctccggcc ggactgcaaa cgcgctgcta tagagaaacg
     3661 tgcgatgtag agcctggcga atgcggtatt cgccgtccaa ccgcgagcgg accggctggc
     3721 cctgggcatc gagccagtca atctgcacgg cgtaggcgcg cccgctggcc ccgcgtgcgc
     3781 ggacgtggat gtcgcgcagt tctgtcaggc cggccgcatc cgtcaggtta acccgcaaca
     3841 attcttcaag ttcagcgcgc gtatatcgca cggcccagcg gaagtagtcg ccgacggcgt
     3901 ccacgcgccc caggtagcgg cttagcgaat caggcgcaac tacgttcggg ctgcaataaa
     3961 cctgcgtccg ggccagccac gcgccgtcca ggtattcctc caggttcgca tcgctcacgg
     4021 ggaagaaccg ccggtcgggc gcatcgggcg gcgcatctac gaccggcctg atacccggct
     4081 tgtcgaggcc ccacacggcc cagggcgtct cggtgacgcc gccgcagcac ttcgaataat
     4141 tcgcgtcaag gatagagcag gaccggtgcg tgtcgccccg acgcgcccca ttggccctcc
     4201 ctcgcgcttc gggctcggat ggcgcccgcg cttcgggtcg cgagcaagcc tcttgatccc
     4261 tcgatccctc gatcccttca tcctttgccc tcgctggcgc ttcgggctcg gattgatcgg
     4321 cccgccctcc cgcttccggc tcggatccgt cgcgcctttc cgcggggctc agcagcacga
     4381 ccccgcgcgt gtcgtgcacg gccgccaacg cggtcgcggt cagcccgccg gtgccctggt
     4441 aacgctggca gcaatcgtcg ttacaccggt caaacggctc gccacgatgc ttgggctcgg
     4501 tcatggccaa caaccacgag cgtgcgacga tggcctgcgc tttcagaaat tcgagcgggc
     4561 aggccgcgct catttcggcc gtaatcacgc ccgccaggta ggtttccagc ggcagcacgt
     4621 tgaccagcac gacgccgtgg cggccgacag acagctccag cgtgccgggc aggatctgcg
     4681 aaacgcgctt ttcccagtga aacccgcggc cggcaggcac gtcgcgcagc agaatacccg
     4741 cctcgcagtc gcccggcttg ctcacgcgca ggtcatcggc gaccggcgcc gcgtgcagcc
     4801 gcagcgaagg cacggtgcgc gcggcttgcc cgctgatgcg gagcgacaga gcaggccggg
     4861 catggcggac ttcgatctcg gtgcccgggt cgatcaccac gtctggcccg cctggactgc
     4921 cgagtacggc ggggcctcgg acgggggtca aatggacggc cgcggctgca tcgccgtcga
     4981 ggaagatgcc cacacgcacc gccggctcta cggccctggc gagcgtccaa tcgcgcaggc
     5041 cgactattcc gtgctccact tccatgcggg caggatatca cgcgggcaga gaatgtggga
     5101 ccacagcggg caggaatgta gggcgggccg cagcaaccgg gcctcccacc gtcctcgggg
     5161 cgggcgcggc gctgttataa tgcacgcacc atgccgtaac tctacgagta catgggaatt
     5221 gtggtgctgt tttattcgaa cgagcacgag ccggtccatg ttcacggtaa gtgccgtggg
     5281 cgggaaagcc gcgctgagct gatcctccgt gagggccgtg tggtggatat catttatacg
     5341 ttggtccgcg accgccagcc gctcgcaggt gtgagcttca ggactttcaa acgctcgtcg
     5401 agcattacgc agatgaaatc gttcagaaat ggatcgatta cttcgtgctc caccaccgtg
     5461 tgcaggcgaa ggtgctgacc aggaggatca aatgagtatc ggcgaaagcg cgccgatctt
     5521 cgtagaacgc gccgagcacg cgggcgagtt cagaatccgc ctcgcattca acgatggcac
     5581 ggtgcgcacc atcgatttcg agccctttct ccgccggtcg cgcaatcctc tgattcaggc
     5641 ctacctcgac ccgggcctgt tctccgcctt ctcgatcaaa gagggcgacc tggtctgggg
     5701 cgattacgac ctctgctttc ccatcgccga cttgtacgac gggcgcctgt gaatcgcgcc
     5761 cgagaactcc cgcgcggaaa gcctcgggct gcgcggcggc aacggcgtcc atccggcaag
     5821 ctccgcgtca aatgtctcgc tcttcggaat gggaccgaag acgattcgca tacattgtca
     5881 tacgcagacg gagcaagccg cacgcaataa gcggaacttg ctccgtcgac aaaaactggc
     5941 atggcctgcg ctggggctcg gatcaaccgg aaccagcgcg atcggcacta tttgcgcgat
     6001 ttcttcgcag ccttcgcctt cctcgcgcca gccttggccc gagccgccgg cttggacttc
     6061 gccgccggtt tggccgggcg cttggccgcc gaagccgctt tcttgctcgt gccgcgcggg
     6121 gctttgccct tcaatggact cggtcccgat tcaatcgccg cctgcgccgc cgccagtctg
     6181 gcaatcggca cgcggtacgg cgagcaactc acgtaattca tgccgacacg atggcagaac
     6241 ttcacactct ccgggtcgcc gccgtgctcg ccgcagatgc cgatcttcag cttgccccgc
     6301 gcccgccgcc cgttttccac cgccatgcgg accagttgcc ccacgccctc ctgatccagc
     6361 gattggaacg ggtcggcctt gaagatgccc gtcttgatgt actcgggcag gaagcggccc
     6421 gcgtcgtcac gcgacaggcc catcgtcatc tgcgtcaggt cgttcgtacc gaagctgaaa
     6481 aactccgccg tttccgcgac cggaccggcc aacagcgccg cccgcggcgt ctcgaccatc
     6541 gtgccgacca ggtacgacag ctcgatgccg gcctccgcga tcacgccgtc ggcaacggcc
     6601 cgcgtgcggt cggccaggat gcgcagctcc ttggggtcga tggtcagcgg aatcatgatc
     6661 tccggattga ccggcaagcc gcgcttcttc aggtcaaccg cggcctcaat gatggcccgt
     6721 acctgcatct ccaggatttc cggatacgtg atgcacagcc ggcagccacg atgaccaagc
     6781 atcgggttcg actcgtgcaa ttgttccgcc cgcgccagga tgtcgttcgg attcacgccg
     6841 gtaacggccg acagccgctc ggcctcttca cgcgtcttgg gcacgaactc gtgcagcggc
     6901 gggtcgatca aacggatcgt gaccgggttg ccttccatcg cctcgaaaat gccgatgaag
     6961 tcgctgcgct ggaacggcag cagtttatcg agcgccgcgc gccgcttggc ctcggtgtcg
     7021 gccaggatca tctcttggat ggcgagttgc cgctcgtggg tggcgaagaa catgtgctcg
     7081 gtgcggcaca ggccgatgcc ttcggccccc atttggatcg ccttgcgggc gtcctcgggc
     7141 gtgtcggcgt tggtacgcac gccgatctcg cgccgctggt ccgcccagcc catgagcgtg
     7201 tagtactcct gcggcggctc gggccgttcg aggtcgagtt tgccttcgta caccgcgccg
     7261 attgagccgt cgagcgtcag gaactcaccc tgccgcacca ccttgccgtt gaccgacatc
     7321 tgcttcgtgc cgtaatcgat ccgcagcgcc tcgcagccca cgatgcagca cttgccccag
     7381 ccacgcgcca ccaccgcggc gtgcgaagtc ttgccgcccg tctgcgtcag gatgcccttg
     7441 gcggcctgca tcccgcccac gtcttccggg ctggtttcct tgcggaccag aatgacgtct
     7501 tcaccttgtt cggccaaagc ctcggcctcg cgcgccgtga agaccacctt gccacaggcc
     7561 gccccgggta cggcgttgat accttcagcg atgcgccgcc gccccagttc ctcgcgcggc
     7621 acgcgctggc tgatgatggg atagaacaag cgttcaatgt cgttgcccgt aatgcgggcg
     7681 atcgcgtcgt cctggctgat taccgggcgc gcggcgcgct cctcgtactt gcgaggcaga
     7741 tagccggcct tcgccaggcg ccgcgcttct tcccgcctca tcaggggctg cgtggcctgc
     7801 tcaacggcga tgcggaaagc ggcgtgcggg gtgcgcttgc cggtgcggca ctgcaggatg
     7861 tacagcttgc cgcgctcgaa cgtgaattcg acgtcctgca tctcgccgta gtgcacttcg
     7921 agcatggccc gcacggcgca aagctggtcg tacatggcgg gcatgcgctc ggccagttca
     7981 ctcatgtgca gcggtgtccg aatgccggcc accacgtcct cgccctgggc attgatcaac
     8041 atgtcgccgt agaactcgtt ggccccggtg ctcggatcgc gcgtgaagca cacgccggtg
     8101 ccgctgtcct cgcccatgtt gccgaagacc atttgttgca cgttgacgcc ggtgcccgtc
     8161 agatcggtga tcttctcgac gcgccgatac gtgacggcct tttcggcgtt ccacgaatta
     8221 aagacggcgt tgatcgcacc gacgagctgc tcccacgggt cctgcgggaa ctcgcgcccg
     8281 gtctgctcgc gaaagaagtc cttgaattgc ccgcacagct cttgcagcgc ttcggccgga
     8341 agctcatagt cgtagcgctt gtcgaggcgt tctttcaggt tgtgcagctt ctcttccaga
     8401 atgtccttgc tcaggccgac ggccgtggtc gagtacatct gcaggaagcg gcggtacgaa
     8461 tcccaggcga agcgcgggtt cttcgtgttg cgggccaggc cctcgacgga ttggtcgttc
     8521 atgccgaggt tgaggatcgt ttccatcatg cccggcatgg aacgggccgc gccgctgcga
     8581 accgaaacca gcagcgggtt gttcttgtcg cccagcttct tgccgactgt cttctccagc
     8641 cgcgcgagct gctggcgaac ttccttttcc aagccctcgg gccaggtgcc gttgtgctcg
     8701 tgaaaatagg cgcacgcatc gaccgcaatc gtaaagcccg ccggaaccgg cagaccgatg
     8761 ttggtcatct ccgccaaccc ggcgcctttg ccgccgagca ggttcttgtc ggtacccttg
     8821 ccctccgcct taccggcgcc gaaaaagtac acaaacttct tcatgtaacg cgatccttac
     8881 aaaaccgtcg taacgcgatc cttacaaaac cgtcgcggaa actaaacggc gtccccagcg
     8941 tcccacggcg gcgacaatcc cacaattata aggccatccc cgaccgcggt caccacgccg
     9001 taacc
//
LOCUS       DAOSYS010000150         8974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6615_length_8974_cov_5.499944, whole genome shotgun sequence.
ACCESSION   DAOSYS010000150 DAOSYS010000000
VERSION     DAOSYS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6615_length_8974_cov_5.499944"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLU99_10075"
     CDS             <1..480
                     /locus_tag="PLU99_10075"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOO17444.1"
                     /translation="DPEAGWLAHARLGFNFRLPDINCAIGVEQIKRLDEIKARRAQVA
                     GWYIERLRDEKRLQLQLVPDEISINWFVFVVRLIDDYTQAQRDEILKKLAQRGIGCGN
                     YFTPIHLQPFYRDQFGYKPGDFPVTEALSARTVALPFHNRLSEGDVDRVTKVLRELL"
     gene            complement(1219..2355)
                     /locus_tag="PLU99_10080"
     CDS             complement(1219..2355)
                     /locus_tag="PLU99_10080"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOO17445.1"
                     /translation="MKLSHKCLTVLALPGLLLGGCLTLPGQPSRVGPGLLAETRVSVA
                     ADPSALAFTSDGRVFYTEKNTGQIRVIDQDGLLAQPFAQVPVNSAGQRGLLGIALHPD
                     FPTVPRVYVFYTRSDSGVSTNNPQAVFDNRVVFFQANGSVADGGEVFVATLPATSATT
                     NVGGQIAFAADGTLLVALGDQAESAAAGDPASLLGKVLRYNDDGSIPADNPSPDSPVF
                     ASGLRNPGGLCVDPASGSVFVIDRGPDGASEINRVTSGADLGWPAVSGAANTSAEQAY
                     VAATPAYIDPLYITPSGELLAGCSFNPSGRYGPDVQNELFFGQATEQRVMIARLDSPR
                     TAIVGVEPFTNSLRGPIHALGFTPAGTLYVATANAILRLAPLPG"
     gene            complement(2426..3865)
                     /locus_tag="PLU99_10085"
     CDS             complement(2426..3865)
                     /locus_tag="PLU99_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOO17446.1"
                     /translation="MVAQQDVLEHYRQTLACYHQEHLLAFWPLLDDRQRDQLLEDLAQ
                     IDFDECSRLIDRLVRQKPVLPLPRSTEPPQVLPSVPRQGSQAEQYEAATALGIQAIQS
                     GQVAAFTVAGGQGTRLGFDGPKGAFQISPIRNAPLFQWFAESLRGVHRRYGACPRWYI
                     MTSPTNDAQTRDFFQKHAYFGLRPDNVFFFAQGQMPAFRHDGRIALAEPHRVALSPDG
                     HGGSLRALRRSGALDDMRAHGIQHISYFQVDNPLVKTIDPLFIGLHIQQNSEMSSKAV
                     TKADDLERVGNFCLADGRLTVIEYSDLPDELAHARNPDGSRRFDAGSIAVHVLAREFV
                     ERLTAENAAIQLPWHRADKKVRIIDEHGRPVEPDQPNVVKLEMFIFDAIPLARNPLVL
                     YTQREEEFSPVKNATGVDSAATARRDLVRRAARWLEQCGCRVPRDADGEPDLPLEISP
                     AVALDAADLCAQLQNAPPVLSPKKPFLLA"
     gene            4549..5571
                     /locus_tag="PLU99_10090"
     CDS             4549..5571
                     /locus_tag="PLU99_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO17447.1"
                     /translation="MKRKGFTLIELLVVVAIIALLISILLPSLARARELTKRSVCMAN
                     LRGIGSGCVIYSNDNNEWFPMSYFAENMSYSESSRGEHVVDFTEGAETNSPGIGANHS
                     LRTTSDGVEGPINTNVPISRSLFLTIIGGQCTPKLFTCASSGENEDVMRNIPASSGGS
                     DQNTVAAQPGINRFDFRGYPNLSYGYQMPFGMHGKPRYGMDPRQPLAADKGPAFAVGG
                     ETGGNLDNLQYTPEARKLGDMAGSGGESDDTDIRFEQDDEEFLLQLGPEIWRPYNSRN
                     HGGEGQNVLIADGHVEYVKMPTVGLNKDNIYTRTTNWTVEGSLLGKFPENRMGPWVNT
                     DSVLIP"
     gene            6084..7604
                     /locus_tag="PLU99_10095"
     CDS             6084..7604
                     /locus_tag="PLU99_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO17448.1"
                     /translation="MKRRAILFLKPYEVCIVSVDPLSINDADLSQHLDQELGEVSSED
                     LARMADAPVSEPQVDARGKLRGRVLAIRGSDVFVDIGGKSEGFVALDEFEPDQPPVID
                     QVLDLVPQGFDKESGLMRLSLREAKLAVDMESIRVGDVVKARVTGSNIGGLELRVHGA
                     RGFMPMSQVDLVRHDDMAGFIGRWLECEVTEVDRKGRGLVLSRRRVLERQREEDARQL
                     RFQLAEGQVRRGKVVRLLDFGAFVDLGGVEGLLHVSDMTWGRVGHPREVLNVGAEIDV
                     KILKIDLVKDRISLGVKQLSPDPWTMVPSKYAVGQTVEGRVLKLMNFGAFVELEPGVE
                     GLIPISEMSWSQHVRNPKDILNVGDNVRTSVLSLDAENRKLSLSLKALSSDPWTGVTE
                     RYPPGTTVSGLVTRITNFGAFVQLEEGIEGLVHISQISAQRIRAVSDAVEVGKVVQVR
                     VLSVDADQRRVSLSMKPLPAPEPERAESAAAGDEATAAPPRPEKKRKRPLRGGLAW"
     gene            7769..8869
                     /locus_tag="PLU99_10100"
     CDS             7769..8869
                     /locus_tag="PLU99_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOO17449.1"
                     /translation="MVLWVLRALFIVLISAVAFSVGGEPGAAAGLGQWRVYLMVGAIC
                     IMVAIIAVDVFIPRKSLAALSGVFLGLVVGMVVAYGLGLVMDLVIQAYAPDVYSAPAD
                     TGAHGLIVGLKLTLGLACCYLFISFILQTKDDIRFVIPYVEFAKQSRGVRPLILDTSV
                     IIDGRIADICNTRIIDSPLIIPRFVLQELQTIADSSDKLKRNRGRRGLDMLNKLQTND
                     KVEIKISDVRLPSVEEAADVDQKLVEMGKKMDGRIVTNDFNLNKIAQIRGVEVININD
                     LANAMKPVVMPGETVTVKIIKPGEEVGQGIGYLEDGTMVVAEGSRDKINSEVTLTVTS
                     VLQTSAGRMIFGRPEGEGYIPPRRARAPRAQA"
BASE COUNT         1757 a         2540 c         3008 g         1669 t
ORIGIN      
        1 gatccggagg ccggctggct ggctcacgcg cgactgggct tcaatttccg tctgccggat
       61 atcaactgcg ccatcggcgt ggagcagatc aagcggctgg acgagatcaa ggcacgccgc
      121 gcgcaggtgg ccggctggta catcgagcgc ctgcgggatg agaagcggct gcagttgcag
      181 ttggtgcccg acgagatttc aataaactgg ttcgtgttcg tcgtgcgtct gatcgatgac
      241 tatacccaag cgcagcgcga tgagattctg aagaagctgg cacaacgtgg catcggctgc
      301 ggcaattact tcacaccgat acacttgcag ccgttctatc gtgaccagtt cggttacaag
      361 ccgggcgact ttccggtgac cgaggcgctg tcggcgcgta cggtagcgct gccattccac
      421 aatcgcttga gcgaaggcga tgtggatcgg gttacgaaag tcctgaggga gttgttatag
      481 gggtcaaaag gcgtctgcga actgcgggat taacatcgcg cgggacttgc gctattacag
      541 atttgcaatc gtctgacgcg atgccggaat cgctcccggc tgcatcaaga cttgcaaacc
      601 actccaatca agcgtagagt cgcggcattg tcggtgaagg ttgccgacac tagcctgcga
      661 gcgtccgata cgagcagtga tcaggtcgta gactgccgtg tcagaacagt gaaatgcagg
      721 acggccatgg atggcggcca ggggacgtaa gccgccgata ttaaggcttg aagcgtcgcg
      781 acagtgtgct tttctgcgct gagcgatcgc tgaaggtacg tttaactgga aaaaggtggc
      841 atgtatggct gagagagcga ataggacgac tagaagcaaa gtgaccagcg ccccgaaggc
      901 cggtggcggc gccgcggcgc gcaagacgac tcgtccggcg ggccggccgc agacgcgcca
      961 agagcttggc atcagcgatg accaagttcg caaccgagcc taccagattt ttctgcggcg
     1021 gaacggcgcc ccaggcgatc aattcggaga ctgggtattg gccgaacatg aattgatgac
     1081 tgaagtgcgc tctcgagctt cccgcgggtc gcgccgcggc cagccgcaag gttgaataac
     1141 agctcccagg tcagcttacc gggacagaca gtcaagcctc acggcagacg ccgctctgtc
     1201 cacagtctcg ctttcgattc agcccgggag gggagccagg cgcagaatgg cgtttgcagt
     1261 agctacgtac agcgttccgg ccggcgtgaa gcccaaagca tgaataggtc cgcgcaagct
     1321 gttcgtgaat ggttcgacgc caactatcgc ggtacgcggg gaatcaagtc tggcgatcat
     1381 cacgcgctgc tcggtcgcct ggccgaagaa gagctcgttc tggacatccg gcccatagcg
     1441 cccactggga ttgaaggagc agccggccag caattcgccg ctgggcgtga tgtagagcgg
     1501 gtcaatgtaa gctggggtgg ccgcgacgta ggcctgctcg gccgacgtgt tcgccgcgcc
     1561 gctcactgcg ggccagccga ggtccgcgcc ggacgttacg cggttgatct cactggcgcc
     1621 gtccgggccc ctgtcaatga cgaatacgga accactggcc ggatcgacgc atagcccgcc
     1681 cgggttgcgg aggccgctgg cgaaaacggg cgaatcgggc gatggattat ccgccgggat
     1741 ggagccgtcg tcgttgtacc tgagaacctt gcccagcagc gacgctggat cgcccgccgc
     1801 ggcagattcg gcctgatcgc cgagcgctac gagcagtgtt ccgtcggcgg caaaggcgat
     1861 ctgaccgccg acattcgtag tcgcggatgt cgctggaagc gtggcgacga atacttcacc
     1921 gccgtcggct acgctgccgt tggcttggaa aaagacgaca cgattatcga acacggcttg
     1981 cgggttattc gtgctgacgc cgctgtccga gcgcgtatag aagacgtaga cacgcggtac
     2041 agtgggaaaa tcagggtgca gggcgatccc gagcaggccg cgctggccgg ccgagttgac
     2101 cggcacttgg gcgaaaggct gcgcgagcag cccgtcttga tcgatgacgc ggatctggcc
     2161 ggtattcttc tcggtgtaga agacgcggcc gtcggaagtg aacgccaggg cgctggggtc
     2221 ggcggccacg ctgacgcgcg tctcggcgag caggcccggc ccgacgcgcg acggctgtcc
     2281 cggcaacgtc aggcagccgc ccaacagcag gccgggcaag gcgagtacgg tcaagcattt
     2341 atgggacaac ttcatctgtt ttccctctcg tggcacggcg gccatgatag atgcgcgcca
     2401 tttggaacgc aaagcatcag cggcgctacg ccaataggaa aggcttcttt ggcgagagca
     2461 ccggcggggc attctgaagc tgcgcgcaca aatcagcggc gtcgagtgca acggctgggc
     2521 tgatttccag cggcaggtcg ggctcgccgt ccgcgtcgcg cggcacgcgg cagccgcact
     2581 gttccagcca gcgcgcggcg cggcggacca ggtcgcggcg ggcagtggcg gccgagtcga
     2641 cgccggtcgc atttttgacc gggctgaact cctcctcgcg ctgggtgtac agcacgagcg
     2701 gattgcgtgc cagcgggatg gcgtcgaaga tgaacatttc cagcttcacg acgttgggct
     2761 gatccggctc gaccggccgg ccgtgctcgt cgatgatgcg caccttcttg tcggcccggt
     2821 gccagggcag ttggatggcg gcgttttcgg cggtcaggcg ttcgacgaat tcgcgcgcca
     2881 ggacgtggac ggcgatgctg ccggcgtcga agcggcggct gccgtcgggg tttctggcat
     2941 gtgccaactc gtcgggcaaa tcgctgtatt cgatgacggt tagacgcccg tcggccaggc
     3001 agaagtttcc gacgcgttcg agatcgtcgg ccttcgtgac ggccttgcta gacatctcag
     3061 agttctgctg gatgtgcaac ccgatgaaca gcgggtcgat tgttttgacg agcgggttat
     3121 ccacctgaaa gtaactgatg tgctgaatcc cgtgggcgcg catgtcgtcc agggcgccgc
     3181 tgcggcgtaa ggctcgcaga ctcccgccgt ggccgtccgg ggataaagcc acgcgatgcg
     3241 gctcagcgag tgcgatgcgg ccgtcgtggc gaaacgcggg catctggcct tgagcgaaga
     3301 agaagacatt atcgggacgc aagccgaagt aagcgtgctt ctggaagaag tcgcgtgttt
     3361 gagcatcgtt cgtggggctg gtcatgatgt accagcgcgg gcaggcgccg tagcgccggt
     3421 ggacaccgcg gagcgattcc gcgaaccact ggaaaagggg ggcattgcga atcgggctga
     3481 tctgaaacgc gcccttgggt ccgtcgaagc cgaggcgggt accctggccg ccggcgacgg
     3541 taaacgcggc aacctggccg gactggatgg cctggatgcc gagggcggtg gcggcctcat
     3601 actgctcagc ctgagaaccc tgtcgtggga cacttgggag gacttgtggg ggctccgtac
     3661 tgcgcgggag gggcaaaacc ggtttttgtc gcacgaggcg atcgatcaag cgggagcact
     3721 cgtcgaagtc gatttgggcg aggtcttcga gtagttggtc gcgctgacgg tcatccagaa
     3781 gcggccaaaa tgccaggagg tgctcctggt gatagcaggc gagcgtctgg cggtaatgct
     3841 cgagcacatc ttgttgtgcc accatgggtt gcgcgctcct gacaatgtgt gataaccacc
     3901 gcgttggcaa aaggaaatta tacacgaact atcggtaagg gtgatattat gggctgaggg
     3961 atttggttga tgagctgcgg attaggcggg cgcggagtgg cttgcgcaac cgctcccggg
     4021 gggctcgccg agaagtccta taacgccaaa ccgaaatttt ccattgactg aggggcccgg
     4081 cgcagttaag ttattgatgg agaaccacaa cacaaaacga caaggagtgc gtgacgggat
     4141 cgaaactggg ttgttcataa gtttgctatc atacatacga taaaatctat ccgattattc
     4201 cgaccctctt tcggttgtca cacagcagtt tgttcccgcc attcccgcac gagctgtcgg
     4261 cgctgagttt atgcgcccgc ttggctcgtt gcttttgctc cctggtcttg gaaatcagtg
     4321 tgtaacccgc gcgctgtccg ccatcccgta cgacagcacc cattcggccc gcgtgtggct
     4381 gggtgaggaa gcgcggcgac cggcccaggc acgaagccct ggtgtacgcc tgccaaggcg
     4441 gttggtccgc atggcagtac gaatgcagtc attaattcaa gaaccgggct cgcgcggcga
     4501 gccgatcgca aaggaacatt acgggtctga ttgcaggagg tttgaacggt gaaaaggaaa
     4561 gggttcacat taattgagct gctggtggtg gtggcgatca tcgcactgtt gatctccatt
     4621 ctgctgccca gcctcgcgcg ggcgcgcgag ctgacgaaac gttcagtgtg catggcgaac
     4681 ctgcggggca tcggcagcgg gtgcgtcatc tactcgaacg acaacaacga gtggttcccg
     4741 atgtcgtact tcgccgaaaa catgagctac agcgagagct cgcgcggcga acacgtagtt
     4801 gactttaccg agggtgccga gacgaactcc ccgggcattg gcgccaacca cagcctgcgg
     4861 actacttcgg acggcgttga agggccgatc aacacgaacg tgccaatcag ccggtcgttg
     4921 ttcctgacga ttatcggcgg tcagtgcacg cccaagttgt tcacctgtgc tagctccgga
     4981 gagaacgaag acgtcatgcg caacatcccc gcttccagcg gcggatcgga ccagaacacc
     5041 gtggcagccc agccgggcat caaccgcttc gacttccgcg gctatcccaa cctgagctac
     5101 ggctaccaga tgcctttcgg aatgcatggc aagccgcgct acggcatgga tccgcgccag
     5161 ccgctggccg ccgacaaggg accagctttt gcggtgggcg gcgagaccgg tgggaacctg
     5221 gacaacctcc agtacactcc tgaagcgcgc aagctgggcg acatggcggg ctcgggaggc
     5281 gaatcggacg acaccgacat caggtttgaa caagacgacg aagaattcct gctgcagctc
     5341 gggccggaaa tctggaggcc ttataacagc cgcaaccacg gcggcgaagg ccagaacgtg
     5401 ctgatcgctg atgggcacgt cgaatacgtt aagatgccca ccgtcggtct taacaaggac
     5461 aacatctaca cgcggacgac caactggacc gtcgaaggct ctctgttggg taaattcccc
     5521 gaaaacagaa tgggtccttg ggtgaatacg gactcggtcc ttattccata gccagtgccg
     5581 caagccctga cgcacggagc cgggcggtgg atcagagggt ccgccgcccg gacttatttt
     5641 tcggacccgt ttacccagca gctaattccg ctcgcaccct gcgcgttgcg tgcgacctgc
     5701 cacgccggca acaagcccca cccgggcggg cggatccgat ggggttaacg gctgccggaa
     5761 ctggctcttg taggtgcagg acgtacacca gcggcctgcg gtttccccga acacttcacc
     5821 actccgtgag gcttcacaac tcgctgagcg ccggaagtcc aagcggcggt agatgcgaat
     5881 tccgccggtt gctggagccg ccgatgatgc tgcgccaccg gcgccggtag agcatccacg
     5941 gccaacggcg aaggctgcgg gccaagttta tctgacgccc agcgccggcg cggcgcgacg
     6001 acacgtagat cgagaaacag ccttggccgg tgacacggtc gagctgctcg aatgcgcttg
     6061 tgtttcgcgc atgaacggtt aacatgaaac gtcgcgcgat attatttttg aagccctatg
     6121 aggtttgcat cgtgtccgtc gatccgcttt caattaacga cgccgatctg agccagcacc
     6181 tggatcagga gcttggcgaa gtatcttcag aggacctggc ccgcatggcc gatgcgccgg
     6241 tcagcgagcc gcaggtggat gcgcgcggca aactccgcgg gcgggtgctg gccatccgcg
     6301 gttcggatgt gtttgtcgat attggcggca agagcgaggg gtttgtcgcg ttagacgagt
     6361 tcgagccgga tcagccgccg gtcatcgatc aggttctcga cctggtaccg cagggcttcg
     6421 acaaggaatc ggggttgatg cggctgtcgt tgcgcgaggc gaagctggcg gtcgacatgg
     6481 agtcgatccg cgtgggcgac gtggtcaagg cgcgggtcac cggcagcaac atcggggggc
     6541 tggaactccg ggttcatggc gcgcggggct tcatgccgat gagccaagtc gatctggtgc
     6601 ggcatgacga catggcgggt ttcatcgggc gctggctgga gtgcgaggtg accgaggtgg
     6661 accgcaaggg ccgcggcctg gtgctcagcc gacggcgggt cctggagcgg cagcgcgagg
     6721 aagacgcccg tcagcttcgg tttcagttgg ctgaaggaca ggtgcggcgc ggtaaagtcg
     6781 tgcggctgct ggatttcggg gccttcgtgg atttaggggg cgtggaggga ttgctgcacg
     6841 tcagcgacat gacgtggggt cgcgttgggc acccccggga agtgctgaac gtgggcgctg
     6901 agatcgacgt caagattctc aaaatcgact tggtgaaaga ccgcatttcg ctgggcgtca
     6961 agcagttgag cccggacccg tggacgatgg tccccagcaa gtacgccgtg gggcagacgg
     7021 tagaggggcg agttctgaag ctcatgaact ttggggcgtt cgtcgagctg gagccgggcg
     7081 tggagggcct gattccgatc agcgagatga gctggtcgca acacgtgcgg aacccgaaag
     7141 acatcttgaa tgtgggcgac aacgtgcgga cctcagtgct gtcgctggat gcggagaacc
     7201 gcaagttgag cctgagcctg aaggccctgt cgagcgaccc ctggacgggc gtgaccgagc
     7261 gctatcctcc cggaacgacc gtcagcgggc tggtgacgcg cattactaac tttggggcct
     7321 tcgtgcagct tgaagaaggc atcgaggggc tggtgcacat atcgcaaatt agcgcccagc
     7381 gcatcagagc ggtttccgac gcggtcgagg tcggtaaagt ggtgcaggtg cgagtcttgt
     7441 ccgtggacgc agaccaacgc cgcgtttcgc tctcgatgaa gcccctgccg gctccggaac
     7501 ccgagcgcgc cgaaagcgcc gccgccgggg acgaagcgac ggccgcgccg cctcgcccgg
     7561 agaaaaagcg caagcggccg ctgcgcggcg gtctggcctg gtaagcacca ggctgagtgc
     7621 ggcacaaaat tgagggacga tgcgcgcggc ttaagattgt gttgtccggg gcggcgaagt
     7681 tcatcgtttc atccggcccg ccgccggcgc gcttgcggcg acagtaaact ggcttcatat
     7741 gtagtagatt cattcaacgg agcagaaaat ggttctgtgg gtcttacggg ctctgttcat
     7801 cgtgctcatt tccgccgtgg ccttctcggt cggcggcgaa cctggggcgg ccgccgggct
     7861 gggacaatgg cgcgtttacc tgatggtggg ggcgatctgc atcatggtgg cgattatcgc
     7921 ggtcgatgtt ttcattcccc ggaaatcatt ggcggcgctc tccggcgttt tcctgggctt
     7981 ggtggttggg atggtcgtgg cctacggcct ggggctggtc atggacctgg tcattcaggc
     8041 gtacgcgccg gacgtataca gcgcgccggc agataccggg gctcacggcc tgattgtggg
     8101 acttaagctg acgctcgggt tggcctgctg ttacctattc atcagcttca tcctgcaaac
     8161 aaaagacgat atccggttcg tcattccgta tgtcgaattc gccaagcagt cgcgcggcgt
     8221 caggccgctg attctcgaca cttcggtgat tatcgacgga cggatcgccg acatctgcaa
     8281 tacgcggatt attgattcgc ctttgatcat tccgcgtttt gtgctgcagg aactgcagac
     8341 catcgccgac agctccgaca agctcaaacg caaccgcggg cgccgcggct tggacatgct
     8401 caacaagctt cagaccaacg acaaggtcga gatcaagatt tccgatgtcc ggttgccgag
     8461 tgtcgaagag gccgcggacg tcgatcagaa gctggttgag atgggcaaga agatggacgg
     8521 ccgcatcgtc accaatgact tcaacctgaa caagattgcc cagattcgcg gggtcgaggt
     8581 gatcaacatc aacgacctgg cgaacgccat gaagccggtc gtcatgccgg gcgagaccgt
     8641 tacggtcaag atcatcaagc ccggcgaaga agtcgggcag gggatcggat acctggaaga
     8701 cggaaccatg gtggtggcgg agggctcacg cgacaagatc aactccgaag tgaccttgac
     8761 cgtgaccagc gtgctgcaga cctcggccgg gcgaatgatc ttcggccgcc cggaaggcga
     8821 gggctacata ccgccgcgtc gtgcgcgcgc cccccgggcg caggcttaag gccggcacga
     8881 acaagtaatc atcgcacggc tgcccgcatg ggttccatgc ccacgccgcc aaaagcgccc
     8941 gcacgggtgc catgctcgag ctgtaagcgc gggc
//
LOCUS       DAOSYS010000151         8954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6628_length_8954_cov_5.503203, whole genome shotgun sequence.
ACCESSION   DAOSYS010000151 DAOSYS010000000
VERSION     DAOSYS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_8954_cov_5.503203"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="ruvB"
                     /locus_tag="PLU99_10105"
     CDS             complement(<1..694)
                     /gene="ruvB"
                     /locus_tag="PLU99_10105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOO17450.1"
                     /translation="MPIERIIGQAGPDEEVYLTSLRPRRLDECIGQEIVREQLRIALD
                     AARQRREPLDHVLLDGPPGLGKTTLAHIIAEEMGAGLHLTSGPAITRQGDLMMMLTQL
                     EAGDVIFVDEIHRLHKVVEEFLYPALEDFRVDYTTESGIGGRTVNFALKRFTLIGATT
                     RAGMLSSALHSRFGLFFHLQYYSTEELTRIVARNAELLKVAAERPALELLASRSRGTP
                     RIVNRLLRRVRDY"
     gene            complement(709..1335)
                     /locus_tag="PLU99_10110"
     CDS             complement(709..1335)
                     /locus_tag="PLU99_10110"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOO17451.1"
                     /translation="MICRITGRLLSVTEDAAVVDVGGICREVLVPRSALGDLSRLNGS
                     DVTLFTLEYLDGNPAMGHLVPRIVGFLTEAERGFFNELQKVKGLGVRKALRAMSVPAH
                     QIARAIESGDERSLANLPEIGKRTATQMVAQLRGKLERFLAPAAGPAPVAQMTAAQTV
                     ALDILVHWGDRRADAQRWIAAAVEADPELKEPDEIVRAAYRIKNAGAI"
     gene            complement(1332..1898)
                     /gene="ruvC"
                     /locus_tag="PLU99_10115"
     CDS             complement(1332..1898)
                     /gene="ruvC"
                     /locus_tag="PLU99_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOO17452.1"
                     /translation="MGIDPGLERTGYAVLCEADGKEARLSEAGLIRLSARQSLESRLA
                     ELHESLEVLLQTHRPTLLACEELYAHYRHPRTAILMGHARGVVLAAAARQGVPVVPIA
                     ATQVKKILTGSGHAAKHQVQFAVAATLGLPQMPEPPDVADAIAIALCGLRLRRLDGAL
                     SGAELRTTNPAARRRSRRSCAAAQEGGR"
     gene            2215..4392
                     /locus_tag="PLU99_10120"
     CDS             2215..4392
                     /locus_tag="PLU99_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOO17453.1"
                     /translation="MHSKLLRLPLLLVLLAASLSARADDNALSPAYAAILRGDVDAGL
                     TELARLRESGAPPAEVARVESWLEAYRKTVDSRAQMKEETFAWNVAEAKKALEQNETY
                     LALSFAAQAVSYAADEQAYSQEPWIVDLTAKAMEQAQQLEAEHRWSKAHRYYWLLKQL
                     HPKDKRFEELRERSERLARLELLYKDKEEFERRIKDVDKNLLRSAVSFINKNYYRDPD
                     FTKMGEGAIDNLLALTETQKTYDYLNGLGNPVTRTSFQEGLKELRAWPRTEERFNSKD
                     LLKLYNRVADLNDKTAELPEGLLVMEFLEGALQQLDEYTSMIWPVDAPDFDKMMMGGF
                     EGVGIQLSLDERSGRLKVVTPLDDSPALEAGVQPDDLIVEVNGQDTKGWSTEDAVRNI
                     MGQAGTEVTLTLFRPSTGQRLTYTLTRRNIVLRTVRGVERQADGQSWNYMLDEETGVA
                     YIRISGFHPDTHEELVQALKAAQRQGMRGLILDLRHNPGGLLDVAVRSVSTFVGNGEV
                     VSTGGRREARTSMRVTDEPFQVKVPMVVLINEGSASASEILAGAFQDHHRAIVLGERS
                     FGKGSVQRVLTLGGSARLKLTTAIYYLPSGRSPHKDPDAEVWGVSPDVEVALTPKEFR
                     QVLKRETDTYIIRSNEAQSATVSEEDRDKALEGLKGKAEDDDEPLLTDEQIKELEGDP
                     VKPQNGDPQLETALLLLRVKLAANLPWPQQLASTGSESAKTQP"
     gene            4573..5931
                     /locus_tag="PLU99_10125"
     CDS             4573..5931
                     /locus_tag="PLU99_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOO17454.1"
                     /translation="MGTLAHEVAQRALALRFDDLSPQTVHEAKRRILDSLGTALGAWN
                     SRPGRVTRDAAAAARTAQGASILGSRQRTLPELAAFANGTLVRYLDFNDTYLSKEPAH
                     PSDNIPAAWAVGQVAGAEPREIITAIVLGYELQCRLCDAASLRAHGWDHVCYGAFSTA
                     LLASRLWGLSVEQTAHALGLAGVCNFATRQTRTGQISDWKACAFANAARNGVFAANLA
                     RLGMTGPHEIFEGPCGLRMVTGPFDLSWSRGPDDWMIRRTHIKRWPAEYHSQSAIDAA
                     LQLRPRIGDMREIESIRIESFEAAVSIIGSEPEKWRPTSRETADHSMAYCVVMALRDG
                     DVTAESFAESNYRDPAVLAWLDRISIVETAECNAGYPDGIPNRLIVKLRDGRTLEQFV
                     TYPRGHARNPMTDQEVIAKFRGLARGVVSDDVAANLVSRALHYDELPDTAELLEFDVL
                     GG"
     gene            5968..7257
                     /locus_tag="PLU99_10130"
     CDS             5968..7257
                     /locus_tag="PLU99_10130"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOO17455.1"
                     /translation="MADKARLIVGTEAYDLPLVVGTENERAVDIAQLRAESGLITLDE
                     GYGNTGSCQSKITFIDGERGILRYRGYPIEQLAAHSTFLETAYLIIFGELPNEQQFRE
                     FRELISEHMFIHEAMRHHFEGFPPNAPPMAILSAMINTLGCFEPQITRPQDNEDFTAA
                     AARLISKVRTIAAASYKKAIGDPIIYPRFDLSYTGNFLHMMFCRPNKLCDPDPAIERA
                     LDQIFILHADHEQNCSTSTVRMVASSQANLYASVAAGVCALWGPLHGGANVAVINMLE
                     AIRQSGESVESFVTKVKDKRAHARLMGFGHRVYKNYDPRAKFLKASTDAVLNKLGIHD
                     PLLDIAVQLEAVALRDPYFIERKLYPNVDFYSGIIMRAAGIPLDMFTVLFAIGRMPGW
                     IANWREVHTDPHGRIYRPRQIYTGPTTRDYVPMSERR"
     gene            7294..8127
                     /gene="prpB"
                     /locus_tag="PLU99_10135"
     CDS             7294..8127
                     /gene="prpB"
                     /locus_tag="PLU99_10135"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HOO17456.1"
                     /translation="MNSRAARLRAALRDHTVVLPGAINALSARLIERSGFEAIYLSGA
                     ALANSTFGLPDVGLTTLTEAAAHAARCAAVTTVPIIMDADTGFGGPENAARTVAEFER
                     AGLSGLHLEDQEFPKRCGHLAGKQLVPTDEFCAKIAAAVSAKRDPDFLIIARTDARGV
                     TSYDDAVARAHAYLKAGADAIFPEALQTREEFERFARDVPAALLANMTEFGRTPYLTV
                     TEFAAMGYRLVIFPVTLQRIAMKAMERALDVLRADGTQRGLLDQMQTRQELYDLLDYE
                     P"
     gene            8235..>8954
                     /locus_tag="PLU99_10140"
     CDS             8235..>8954
                     /locus_tag="PLU99_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOO17457.1"
                     /translation="MTSTTAEIYSPGLEGVIAGETAICCVDQDRLLYRGYDISDLAEH
                     ASFEECAFLLLHGELPTAKQLELFREQIEGERELPGAVVDVLRAIPRQAPMMDVLRTG
                     VSLCGNFSSAKSQGRSSLITRAVRVLAEVPSLIAARLRVMEGKWPVEPRPGLSHAAQF
                     FWQAFQREPTPLEEKVLNLTLVLYAEHEFNASTFAARVCTSTLSGLHSSVVAAIGTLK
                     GPLHGGANEAALQMMLPFSSAA"
BASE COUNT         1660 a         2869 c         2829 g         1596 t
ORIGIN      
        1 cgtagtcgcg cacgcggcgc agcagccggt tgacgatgcg cggcgtgccg cgcgaacggc
       61 tggccagcaa ctcgagggcg gggcgctcgg cggcgacttt caacagctct gcattgcggg
      121 ccacgatgcg cgtcagttcc tcggtgctgt agtactgcag atggaagaac aggccaaagc
      181 gactgtgtag agcgctggaa agcattcccg cgcgagtcgt ggcgccgatg agcgtgaagc
      241 gtttgagcgc gaaattcacc gtgcgcccgc cgatgccgct ttcggtggtg tagtcaacgc
      301 ggaagtcctc cagcgcggga tagaggaact cctcgacgac tttgtgcagg cgatgaatct
      361 cgtccacgaa gatcacgtcg cccgcctcaa gttgcgtcaa catcatcatc aggtcgccct
      421 ggcgcgtgat ggccgggccg ctggtcaggt gcaagccggc ccccatttcc tcggcgatga
      481 tgtgcgccag cgtggtcttg cccagtccgg gcggcccgtc gagcagcacg tggtccaagg
      541 gttcgcggcg ttggcgggcg gcatcgagcg cgatccgcaa ttgctcgcgc acgatctcct
      601 gcccaatgca ctcgtcgagg cggcgcgggc ggagcgaggt caggtagact tcctcgtccg
      661 gtccggcttg tccgatgatg cgttcgatgg gcacgtgtgc acctcgcttc agatggctcc
      721 ggcgttcttg atgcggtagg ccgcccgcac gatctcatcc ggctctttca actccgggtc
      781 ggcctcgacc gcggccgcga tccagcgctg cgcgtcggcc cgccggtctc cccagtgtac
      841 gagaatgtcc aaggccacgg tttgggcggc ggtcatttgg gccacggggg ccggcccggc
      901 cgccggggcc aggaagcgct ccagtttgcc gcgaagctgt gcgaccatct gcgtggcggt
      961 gcgcttgccg atctccggca ggttggccaa ggagcgctcg tcgccgcttt cgatagcccg
     1021 cgcgatctga tgtgccggaa cgctcatagc gcgcagcgcc ttgcgcactc cgaggccctt
     1081 gaccttctgc agctcgttga agaagccgcg ctctgcctcg gttagaaaac cgacgatgcg
     1141 cggaaccaaa tgtcccatcg ccggattgcc gtccagatat tccagcgtga agagcgtcac
     1201 atccgaacca ttcaaccgcg acaggtcgcc cagcgccgag cgcggcacaa gcacttcgcg
     1261 gcagataccg cccacgtcga ccacggcggc gtcttcggtg acgctcaaca atcgtccggt
     1321 aatgcgacag atcaccgtcc accctcctgg gccgcggcgc atgaacgccg cgatcgccgc
     1381 cgcgccgccg ggttcgttgt ccggagctcc gccccagaca gagcaccgtc cagacgacgt
     1441 aaacgcaagc cgcacaacgc aatggcaatc gcgtccgcca cgtccggcgg ctccggcatc
     1501 tgcggcagtc cgagcgtcgc ggccaccgca aactgcactt gatgtttggc ggcgtgcccg
     1561 ctgccggtca gaatcttctt aacctgcgtg gcggcgatcg gaacgaccgg cacgccctga
     1621 cgtgccgcgg ccgccaggac cacgccgcgc gcgtgtccca tgaggattgc ggtgcgcgga
     1681 tgcctgtagt gcgcgtaaag ttcctcgcag gccagcagcg tcgggcggtg cgtttgaagc
     1741 agcacttcca ggctttcgtg cagttctgcc aaccggcttt ccaatgattg acgcgcagac
     1801 agccggatca gccccgcttc ggagaggcgg gcttccttcc cgtcagcctc gcaaagcacg
     1861 gcgtaaccgg tgcgctccag ccccgggtcg attcccagga ccggtcgacc gtcggccggc
     1921 gccggctcgc ccagggttga ccgcccggat aactgcctca atctcctgcc gcacttaccc
     1981 atcgccgacc atcatgcatg ttttttccgg gctgacaacg gcgccggctt cacccgggcc
     2041 gcggggggcg tcggccccgg aaacgctatt ccgtacgatc ttgtcgtttg tagccgatga
     2101 agctttttgg ccgtaggcga gcgccgcgct aggtcgtata attgccgtgg cgaccacgag
     2161 agcctagggc gtgaagctgc ccccctgcga aaaaacgaac aaaggatgag atttatgcat
     2221 tcaaagctcc tgcggctgcc gctgctgctc gtgctgctgg ctgcgagttt gtcggcccgc
     2281 gcggacgaca atgcgttgtc gccagcgtat gccgcgattc tccgcggtga cgttgacgcg
     2341 ggcctgacgg agttagcccg cttacgggag agtggcgccc cgccggcaga agtggcgcgg
     2401 gtcgagtcct ggctggaagc ctatcgcaag acggtcgact cgcgggctca gatgaaggag
     2461 gagacgttcg cttggaacgt cgccgaagca aagaaagccc ttgagcagaa cgagacgtat
     2521 ctggcgctga gcttcgcggc ccaagcggtg tcctatgcgg ccgacgaaca ggcctactca
     2581 caagagccct ggatcgtcga tttgaccgcc aaagccatgg agcaggcgca gcagctcgaa
     2641 gccgagcatc gttggagcaa ggcccaccgt tactactggc tgctcaagca actgcacccc
     2701 aaggacaagc gctttgagga gttgcgggag cgctccgagc gactggcgcg gttggagctg
     2761 ctgtacaagg acaaggaaga gtttgaacgc cgcattaagg acgtcgacaa aaacctgctg
     2821 cgcagcgccg tgagcttcat caataagaat tattaccgcg atccggattt cacgaagatg
     2881 ggcgagggcg ctatcgacaa cctgctcgcc ttgaccgaaa cgcagaagac ttatgattac
     2941 ctgaacggct tgggaaatcc ggtaacgcgc acgagctttc aagaaggctt gaaagagctg
     3001 cgcgcctggc cgcgcaccga ggagcgcttc aattccaagg acctgcttaa actctacaac
     3061 cgggtggcgg acctgaatga caagaccgct gaactgcccg aagggctgct ggtgatggag
     3121 ttcctcgaag gcgccttgca gcagctcgac gaatacacct cgatgatctg gccggtcgac
     3181 gcgccggatt tcgacaagat gatgatgggc ggcttcgagg gcgtcggcat tcagctcagc
     3241 ctggatgagc gcagcggccg gttaaaggtc gtcacgccct tggacgactc tcccgcactg
     3301 gaggcgggcg tgcagccgga cgacctgatc gtcgaggtca acggtcagga caccaagggt
     3361 tggtccaccg aggacgccgt tcggaacatc atgggacagg ccggcacgga agtgaccttg
     3421 acgctcttcc gccccagcac cgggcagcga ctaacgtata cgctgacgcg ccgcaacatc
     3481 gtgctgcgga cggttcgcgg cgtagagcgc caggccgacg ggcaaagctg gaattacatg
     3541 ctggatgagg aaaccggcgt cgcatacatc cgcattagcg gttttcatcc cgacacgcac
     3601 gaagaactcg tgcaggccct caaggccgct cagcgacagg gcatgcgcgg cctgattctg
     3661 gacctgcgcc acaaccccgg cggattgctg gacgtggcgg ttcgttcggt cagcacgttc
     3721 gttggcaacg gcgaagtggt gtcgaccggc ggcaggcgcg aggcgcgcac cagcatgcgg
     3781 gtgacggacg agccgttcca ggtgaaggtg ccgatggtcg tgctgatcaa cgagggcagc
     3841 gccagcgcgt cggaaatcct ggccggggcc ttccaggatc accatcgagc gatcgttctt
     3901 ggcgagcgct cgttcggcaa aggcagtgta cagcgcgtac tgacgctggg aggcagcgcc
     3961 cgcctgaagc tgacgaccgc catttactac ctgcccagcg gccgctcgcc gcataaggac
     4021 cctgatgcgg aggtctgggg cgtttcgccg gacgtggagg tcgctctgac gcccaaggaa
     4081 ttccgccagg tgctgaaacg cgaaacggac acctacatca tccgcagcaa cgaagcacag
     4141 tcggcgactg tcagtgagga agatcgcgac aaggcgctgg aaggcctgaa gggcaaggcc
     4201 gaggacgacg acgagccgtt gctcaccgac gagcagatca aagagctcga aggcgacccg
     4261 gtcaagccgc aaaacggcga cccgcagttg gagacggccc tgctgctgtt gcgcgtgaag
     4321 ctggccgcga acttgccgtg gccccagcag cttgccagca ccgggagcga atcggccaag
     4381 acgcagccgt agggcgcctc ctcgccgcgc ctaattaacg ggatcgagga ttacgggacc
     4441 gagaaaaggc gccgccgtgt gccttttccc ggtcccttct tatttccctt tgtcgctctc
     4501 attatgcggg ttgccgcgga ccggccttgg gtagagaatc agggtgttcg ggttctcagc
     4561 aggaggtgaa cgatgggcac actggcgcac gaagttgcgc agcgcgcgct ggcgctgcga
     4621 tttgatgatc tgagtccgca gacggtccac gaggcgaagc gccgcatttt ggactcactt
     4681 ggaactgccc tgggggcctg gaatagccgg ccggggcgcg taacccgcga cgcggccgct
     4741 gccgcccgca ccgcgcaagg cgccagcatt cttggcagcc ggcagcgcac gttgccggag
     4801 ctggcggcct tcgccaacgg cacgctcgtc cgctacctgg atttcaacga cacgtacctg
     4861 agcaaggaac cggcgcatcc cagcgacaac atcccggccg catgggctgt cggccaggtg
     4921 gccggcgccg agccgcgcga gatcatcacg gccatcgtgc tgggatatga gctgcagtgc
     4981 cgactgtgcg acgccgcttc cctgcgggcg cacggctggg accacgtgtg ctatggggcc
     5041 ttctcaaccg ccctgctggc cagccggctt tggggcctgt cggttgagca gaccgcgcac
     5101 gcgctgggtc tggcgggcgt ctgcaatttt gcgacccgcc agacgcgtac cggacaaatc
     5161 tcggactgga aggcgtgtgc tttcgcgaac gccgcccgta acggcgtgtt cgcggccaac
     5221 ctggcgcggc ttggaatgac cggcccgcac gagatctttg aggggccctg tggcctgcgg
     5281 atggttaccg gcccgttcga tctgagctgg agccgcgggc ccgacgactg gatgatccgc
     5341 cgcacgcata tcaagcgctg gccggccgaa tatcactcgc aatctgcgat cgacgcggcg
     5401 ctgcaactcc ggccccggat tggagatatg cgcgaaatcg agagtatccg gattgaaagt
     5461 ttcgaagccg ctgtaagtat cattggtagt gagccggaaa agtggcgccc gaccagtcgc
     5521 gaaacggccg accatagtat ggcctattgt gtggtcatgg ctctgcgtga tggtgatgtg
     5581 acggccgaat cttttgcgga aagcaactac cgcgatccgg cggtgctggc gtggctggac
     5641 cgcatcagca ttgtggaaac cgcggaatgc aacgccggtt atccggacgg cattccgaac
     5701 cgcctgattg tcaagctgcg cgacgggcgg acgctcgagc agttcgtcac gtacccgcgc
     5761 ggacacgctc gtaaccccat gaccgatcag gaagtgatcg ccaagttccg cggcctggcg
     5821 cgcggggttg tctcggatga cgtggccgcg aacctcgtgt cacgagcgct tcactatgac
     5881 gagctgcccg atacggccga gttgctggag ttcgacgtcc tgggcggctg accgccggcc
     5941 ggcgctaccc gttttcgaga aagtattatg gcagataagg cacgattgat tgtcggcact
     6001 gaggcttatg acctcccact ggtggtcggc actgaaaacg aacgcgccgt ggacatcgcc
     6061 cagctccggg ccgaatccgg tctgatcacg ctggacgagg gctacggcaa caccggttcg
     6121 tgccaaagca agattacgtt catcgacggc gaacgcggca ttctgcgcta ccgcggctac
     6181 ccgatcgaac aactggccgc acactccacg tttctggaga cggcctattt gatcatcttc
     6241 ggtgaactgc cgaacgagca gcagtttcgc gagtttcgcg aactgatctc cgagcacatg
     6301 ttcattcacg aggccatgcg ccatcacttc gagggctttc cccccaacgc tccgcccatg
     6361 gccatcctgt cggccatgat caacaccctc ggctgttttg agccgcagat cacgcgcccg
     6421 caggacaacg aggacttcac cgctgccgcg gcccggctga tcagcaaagt gcgcaccatt
     6481 gcggccgcct cctacaagaa ggccatcggc gacccgatca tttatccgcg ctttgatctg
     6541 tcctacacgg gcaatttttt gcacatgatg ttctgccggc ccaacaagct ctgcgatccc
     6601 gatcccgcga tcgagcgtgc cctggaccag attttcatcc tgcacgctga tcacgaacag
     6661 aactgctcca cgtcgacggt gcgcatggtg gcctcttcgc aggccaacct gtacgcgtcg
     6721 gtcgcggccg gcgtgtgcgc gctgtggggc ccgctgcacg gtggggcgaa cgtggccgtg
     6781 ataaacatgc tggaagcaat ccgccagagc ggcgagtcag tcgagtcttt cgtgactaaa
     6841 gtgaaggaca agcgggccca tgcgcgactg atgggctttg gacatcgcgt ctacaagaac
     6901 tacgacccgc gcgccaagtt cctgaaagcc tcgaccgacg cggtgctgaa taagctggga
     6961 atacacgatc cgctactcga cattgccgtg cagctcgaag ccgtcgcatt gcgcgacccc
     7021 tacttcatcg agcgcaagct gtaccccaat gtagacttct acagcggtat aatcatgcgg
     7081 gccgccggca taccgctgga catgttcacc gtgctctttg ccatcggccg tatgcccggc
     7141 tggattgcaa actggcgcga ggtccacact gacccgcatg gccgcattta ccggccgcga
     7201 cagatataca ccgggccgac cacgcgcgac tacgtaccga tgagcgaacg ccgctagatc
     7261 ggcccgggct tgcggaggac gaaagtggcc cacttgaatt cgcgtgccgc ccgcttgcga
     7321 gccgccctgc gcgaccacac cgtcgtgctt ccgggggcca tcaacgccct gtctgcgcgg
     7381 ctgatcgagc gcagcgggtt cgaagccatt tatctctccg gcgccgcgct ggctaatagt
     7441 acgttcggcc tgcccgacgt gggcttgacg accttgaccg aagcggctgc tcacgccgcc
     7501 cgctgcgcgg cggtcactac cgtcccgatc atcatggatg ccgacacggg cttcggcggc
     7561 ccggaaaacg ccgcccgcac cgtcgcggaa ttcgagcggg ccggcttgag tgggctgcac
     7621 ctggaggacc aggagtttcc caagcgctgc ggccacctgg ccggtaagca gcttgtacca
     7681 accgacgaat tttgtgccaa gatcgccgcg gccgtttccg ccaagcgcga tccggatttc
     7741 ttgattatcg cccgcactga cgcgcgcggc gttacatcgt acgacgacgc agtggcccgc
     7801 gcccacgcct acttgaaggc cggcgccgac gcgattttcc ccgaagccct gcaaacccgc
     7861 gaggaattcg agcgcttcgc ccgcgatgtg ccggcggcac tgctggcgaa catgaccgaa
     7921 ttcggacgca caccatacct gaccgtcacg gagttcgcgg ccatgggcta ccgcctcgtc
     7981 attttcccag tcacactgca acgcatcgcg atgaaggcca tggagcgggc ccttgacgta
     8041 ttgcgagccg acggcacgca gcgcggcctc ttggaccaga tgcagacccg gcaagagcta
     8101 tatgatctgt tggactacga accgtgactt ctggtctggg tatgtaagat tgtgattgca
     8161 gccccccaaa aggctgactc gcgtcacctg cccctcttcc cacattcccg ctatttaatg
     8221 tccggagcgc atccatgact tccaccaccg ccgaaatcta ctctcccggc ctcgagggcg
     8281 ttatcgccgg tgaaaccgcg atctgctgcg tcgaccagga tcggttgcta tatcgcgggt
     8341 acgacatctc cgacttggcc gagcacgcca gtttcgagga atgtgccttt ctgctgttac
     8401 acggcgagtt gcccacggct aaacagcttg aactctttcg cgaacaaatc gagggtgaac
     8461 gcgagttacc tggcgccgtg gtcgatgtgt tgcgcgccat tccacgtcag gcgccgatga
     8521 tggacgtgct ccgcaccggc gtctcgctgt gcggaaactt cagcagcgcc aaatctcaag
     8581 gccgcagtag cttgatcacg cgggcagtgc gcgtgctggc cgaagtgccg agcttaatcg
     8641 ccgcccgcct gcgcgtgatg gagggtaaat ggccggtcga accgcgcccg ggcctgtcgc
     8701 atgcggccca gttcttctgg caggcgtttc agcgcgagcc gacgccgctt gaggagaagg
     8761 tgctcaacct gacgctcgtc ctttatgccg aacacgaatt caacgcctcg acgttcgccg
     8821 cgcgcgtctg taccagcacg ctgagcgggt tgcactcgtc ggtcgtcgcg gccatcggca
     8881 cgctcaaggg cccgctgcac ggcggagcca acgaagccgc cctgcagatg atgctcccgt
     8941 tctcctcggc cgcc
//
LOCUS       DAOSYS010000152         8893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6683_length_8893_cov_4.838764, whole genome shotgun sequence.
ACCESSION   DAOSYS010000152 DAOSYS010000000
VERSION     DAOSYS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6683_length_8893_cov_4.838764"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PLU99_10145"
     CDS             complement(<1..1822)
                     /locus_tag="PLU99_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17458.1"
                     /translation="MKVWHRYLSISVLAATLLLPGAVRGADKAQVAFYQDAEAVTYVD
                     DSTRLCGPDSMTQSSAPDVLHSGSIYCPGSSGTTQAASFARCFNISAASFPEGITVNC
                     LHFGVQQSTVAASLTINIYADHNGCPPLTADLELLGTMNFTVPAGTAMQFFTADFSGI
                     GGVFVPPNTAMVVEIAVPDLNGVGSFRIGANHSGESAPGYVKTAPCSLPDYVSLDSIG
                     YGSVHLAWRVDYFAGGEAPVGACCYPNGQCSFGEQLVCVVEGGAFQGLDTLCEDVSCP
                     QPDLGACCTAAGVCTIDDAWDCGRADGTWLGADTVCGGDCDEDGASDACEIALDMAKD
                     CNENGIPDDCDIASGYSCDGNGNGIPDECDVWMRGDMNCDGVTNAFDIDPFVVALTDL
                     VAYHENWPCCSYLNADCNYDGVVNAFDIDAFVQILTGPPPPIRAVELAGNPLTDYPYF
                     EYVRAFHVNAPISLAVDPTRYPAIRGRTADVYVVEKKTPAEWANDPQLVDVTAGGQMT
                     LTFTGSTIEGNTFQIVGPNELSAMVYQAETGAYTGFGHGYDMVIDINRNGVLDGGDYI
                     DGLSREAGLYVCHDSTTRGPLAVTETLYNVGTIFGIPASVN"
     gene            complement(2356..3141)
                     /locus_tag="PLU99_10150"
     CDS             complement(2356..3141)
                     /locus_tag="PLU99_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17459.1"
                     /translation="MRQRELSDQEPQSTGVPAAGPVVRLQNVSKSFGRKAAVHNLSLE
                     LQAGEVFAFLGPNGAGKTTTLKITTGLLRPDCGQVHVCGHDLVGAARLAKQGLAYVPD
                     EPFIYEKLTGREFLQFTWEMYGVPPDVAIQRLEELSARLSMFEFLDRLAETYSHGMKQ
                     RVALAAALIHAPSVLVVDEPMIGLDPRTIRIAKDMFRELAARGGTVFMSTHTLDIAEQ
                     VADRIGIIHHGRLVALGTLAELRRQAALDGRLEDVFLHLTQGD"
     assembly_gap    3160..3259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3260..3828)
                     /locus_tag="PLU99_10155"
     CDS             complement(<3260..3828)
                     /locus_tag="PLU99_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17460.1"
                     /translation="MLNRWIGIGVVLAMLAGNTSLFVRDILPRWTAGDPPRVEPQAGD
                     NLLYERQQIGIFDAHDRCIGHSWTQIQTLERYVSVSSTTLLNSIDLPDGLHTPKVRVD
                     SELRYTGPDQVLDELTITLHGLPLTVMLRGELMPPDQFACRWQAGREQGVFVLDARLT
                     RAFGDVLRPFMRLPGLYVGQTWRLQLMNPL"
     gene            3979..5202
                     /locus_tag="PLU99_10160"
     CDS             3979..5202
                     /locus_tag="PLU99_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011133920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOO17461.1"
                     /translation="MDLTILVVFCLVYMGMILGRFPGLAIDRTGVALLGAIALVATGR
                     LTPEAAWNAIDVPTITLLLSLMVVSAQFRLGGFYSVVAQRLSTAQVSPSVLLLLLIGV
                     SALLSAVLANDIVCLAFTPLLIEGCVRRGLKPVPFLLALACASNVGSAATLIGNPQNM
                     LIGQKLNLSFSGYLLDGVLPAALGCVAIWLLICWLVHGQWRQRPGTIDIPAPHFDLWQ
                     TAKGSVVLAGLMAAFLFAPWPREVVALAAAGLLLMSRYLHTRRMLAQVDWQLLVLFAG
                     LFVVNHALAASGMLAQGMERLHAAGVAVEQPVWLFGTTVVLSNLVSNVPAVMLLLPAA
                     THPQAGAILALASTLAGNLFIVGSIANIIVIDQAERLGIRITWREHARVGVPVTLATL
                     AIAGAWLYFRAGAQC"
     gene            complement(5408..5797)
                     /locus_tag="PLU99_10165"
     CDS             complement(5408..5797)
                     /locus_tag="PLU99_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HOO17462.1"
                     /translation="MWDDLMDELDRKRIEAETIRALRTIFDPEIPLNIYDLGLIYGVD
                     VSEQGAVHIRMTLTSPLCPVAGSMPAEVENRVRLVHGVTDVKVELVWDPPWTLESLPE
                     AARLALGLDDPAARQPRFPFDFNRNKP"
     gene            complement(5873..6355)
                     /locus_tag="PLU99_10170"
     CDS             complement(5873..6355)
                     /locus_tag="PLU99_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOO17463.1"
                     /translation="MSELDELYQQLILDHYRRPRNRRTLPQADRMAEGYNPLCGDHVT
                     VFLKMDSDLVKEVTFEGSGCAISTASASLMTETLKGKTLAEARAIFRRFHGLVTGQPQ
                     EPLGGPGPVGGPGLGKLEVFSGVRKYPARVKCATLAWHTLDAALENRGEVVSTESDEL
                     "
     gene            complement(6352..7614)
                     /locus_tag="PLU99_10175"
     CDS             complement(6352..7614)
                     /locus_tag="PLU99_10175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOO17464.1"
                     /translation="MSTIPTLVRDTPSEAPLEVRRIRADFPALLQEVNGHPLVYLDNA
                     ATTQKPQVVIECIRDYYERDNANVHRGLHALSMRATEAYEAARHKVQRFINAGQAEEI
                     VFVRGATEAINLVAQTFGRAQVRAGDEILISEMEHHSNIVPWQMLCEQTGAVLRVVPM
                     DDRGELRMEEYEHMLGPRTRLVAVTHVSNALGTVNPVEHVIARAHSAGAVVLVDGAQA
                     VPHMPVDVRALDCDFYVFSSHKTFGPTGVGVLYGRYELLESMPPYQGGGEMISAVTFE
                     KTTFREPPARFEAGTPNIAGAIGLGAAVEYLERLGRDAIMAYERDLLAYGAAALGEIP
                     QVRLIGTASEKAGILSFVLKDIHPHDVGTVLDTLGIAIRSGHHCAQPVMQHFGVPATC
                     RAALAFYNTHEEIDVLVAGIRQVVKVFS"
     gene            complement(7611..>8893)
                     /gene="sufD"
                     /locus_tag="PLU99_10180"
     CDS             complement(7611..>8893)
                     /gene="sufD"
                     /locus_tag="PLU99_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOO17465.1"
                     /translation="EGRLDGGRPWLRELRQAALARFLERGFPTTRDEDWRFTNVSALE
                     RTPFRPAAACDLPAQRAMLRALPWDVGGEQMILVNGRYAPDLSTLSAENGVRCGSLGS
                     ALATGDGELEDYLAQHADYMDCSFVALNTAFMADGAYVRIPRRHMAERPLHIVHISVP
                     GEAPSMVHPRTLVLVDDEAQASIVESYISLGEGTSFTNAVTELVVGERAQLRHYKLAR
                     EALGTYHIGTVQIHQKRDSEVLSHCITLGGGLVRHNINSVLAGAGGHCRLLGLYLVGG
                     DQHVDNHLRVEHVAPHCDSREVFKGILEGRARAVFTGRIVVHRGAQQTDAKQTNKNLM
                     LSGEAQVDSKPQLEIHADDVKCTHGATIGQIDRDALFYLRSRGLSEAAARSLLVYAFA
                     SESLAQVEIESLRRRLQRELADRLPQGEFLEALS"
BASE COUNT         1546 a         2930 c         2649 g         1668 t
ORIGIN      
        1 cgttcacact ggccggaata ccgaaaatcg tgcccacgtt gtagagcgtt tccgtaaccg
       61 ccaacggccc gcgcgtcgtg gaatcatggc acacatacag gccggcctcg cggctcaatc
      121 cgtcgatgta gtcgccaccg tccagcacgc cgttgcgatt gatatcaatg accatgtcgt
      181 agccgtgccc aaaaccggta taggcgccgg tctcggcctg gtaaaccatc gcgctcagct
      241 cgttcgggcc cacaatctgg aacgtattgc cctcgatcgt ggagccagtg aaggtcaggg
      301 tcatttgccc gccggccgtc acgtccacca gttggggatc attggcccac tcggcgggcg
      361 tcttcttctc cacgacgtac acgtcggccg tgcggcccct gatggcgggg tagcgcgtcg
      421 ggtccaccgc cagactaatg ggggcgttca cgtggaaggc gcgcacgtac tcgaaatacg
      481 ggtagtcggt cagcggattg ccggccaact cgacggccct gatgggcggc ggcggaccgg
      541 tcagaatttg cacgaacgca tcaatgtcga acgcatttac cacgccatcg tagttgcagt
      601 ccgcattgag gtagctgcag cacggccagt tttcgtggta ggccaccaga tccgtcaacg
      661 ccaccacgaa cggatcaata tcgaaggcgt tggtcacgcc gtcacagttc atgtcgccgc
      721 gcatccaaac atcgcactcg tctggaatgc cgttgccgtt gccgtcgcaa ctgtagccgc
      781 tggcgatatc gcagtcgtcc ggaatgccgt tttcgttgca gtccttggcc atgtccaagg
      841 cgatctcaca ggcgtccgaa gcgccgtcct cgtcgcagtc gccgccgcag acggtgtccg
      901 cacccagcca ggtgccgtca gcccgcccgc aatcccaagc gtcgtcgatc gtgcagacgc
      961 cggctgcggt gcagcaggcg cccaggtccg gctgcgggca agaaacatcc tcgcacagcg
     1021 tgtccagccc ctggaacgct ccgccctcaa ccacgcacac gagttgctcg ccgaacgagc
     1081 actggccgtt cggatagcag cacgccccga ccggcgcctc gccgcccgcg aaatagtcca
     1141 cgcgccacgc caagtgcacc gagccgtagc cgatgctgtc cagcgagacg tagtccggca
     1201 gagagcacgg agccgtcttg acgtatccgg gagcgctctc gccgctgtga ttggccccga
     1261 tcctgaagct ccccacgccg ttcaggtcgg gaaccgcgat ttccaccacc atggccgtgt
     1321 tgggcgggac gaacacgccg ccgatgcccg aaaagtcggc cgtgaaaaac tgcatcgccg
     1381 tccccgccgg gacggtgaaa ttcattgtcc ccaacaactc caggtcggcg gtgagcggcg
     1441 ggcagccatt gtggtccgcg tagatgttga ttgtcagcga cgcggccacg gtggactgct
     1501 gcacgccaaa gtgcaggcag ttgacggtga tgccctcggg gaagctggcg gccgaaatgt
     1561 tgaagcagcg tgcgaagctg gctgcttgcg tggtgccgct ggagcccgga cagtagattg
     1621 agccgctatg gagcacgtcg ggggcgctgc tctgcgtcat cgaatccggg ccgcagagac
     1681 gcgtggagtc gtcaacgtac gtaacggctt ctgcatcctg ataaaacgcg acttgcgctt
     1741 tatccgcgcc gcgcaccgcg cccggaagca gcagtgtcgc ggccagaact gaaatactga
     1801 gatagcgatg ccacactttc aagacaggcc ctcctatgta gaacctggag cagggaaacc
     1861 acagcgcggt ttgggctgcg cccacacacg tctgagggcg ttgccgcggt aaagagccgc
     1921 gcgcatcatt tctaaagctg atacgccgtt ccggtcccat atctataaca gaaacctgcc
     1981 cggcaaatca atgtaaatgc gcagtttttc gggcaggtcc agaaggtccc gataaaactc
     2041 tggtgcgggc agcaatgcgg cggcaccacg cctgggtcaa agttccgcct gccgacaaca
     2101 agtcggtcac gcggcgccga tttccctcta accctggtta agccatcgac gttccaggta
     2161 attcgaaatg agccgcggtg cgcatggcgc gatgcggcgc aacttccgcg cttgcgaacc
     2221 gcatccgcga gccatcgccc gcaggcgttt ctgtgccgtt cgcgaattcg cttatcggcc
     2281 cttcatcgtc ggcgtgtcta accaaacggg ccggatggcc aacgcaccaa cgcccctcga
     2341 catcgctgtt gcgccttaat ccccctgcgt gagatggaga aagacgtcct ccagccgccc
     2401 atccagcgcc gcctgccgcc gcaactcagc gagcgtgccc agcgccacca gtcgcccgtg
     2461 gtggatgatt ccaatgcggt ccgcgacctg ctctgcgatg tccagtgtgt gagtgctcat
     2521 aaacacagtt ccgccgcgcg cggccaactc ccggaacatg tctttggcaa tccggatggt
     2581 gcgcgggtca agcccgatca tcggttcgtc caccaccagc acgctcggag cgtgaatcaa
     2641 cgctgccgcc agcgccaccc gttgcttcat gccgtgactg taggtttccg ccagccggtc
     2701 cagaaactcg aacatgctca gccgggcact caactcctcc agccgctgga tagccacgtc
     2761 cggcggcacg ccgtacatct cccaagtaaa ctgcaagaac tcgcggccgg tgagtttctc
     2821 ataaataaaa ggctcatcag gaacgtatgc caaaccctgc ttggccagac gggcggcccc
     2881 caccaggtca tgcccgcaaa cgtgtacctg gccgcaatcc gggcgcagca ggccggtcgt
     2941 gatcttcagg gttgtggttt tgcccgcgcc gtttggaccc aggaacgcaa acacttcgcc
     3001 ggcttgaagc tccaacgaca gattgtgcac cgccgccttg cgcccaaagg acttcgagac
     3061 gttctgcagc cgcaccaccg gccctgcggc tggcacgcca gtcgattgcg gctcctggtc
     3121 gctcaattcg cgctgtcgca tatcgttttc cggttcacgn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnna gcgggttcat caactgcagc cgccacgtct gcccgacata
     3301 caggccgggc agccgcataa agggccgcaa tacatctccg aaggcccgcg tcaagcgggc
     3361 atccagcacg aacacgcctt gctcgcggcc ggcctgccag cggcaggcga actgatcggg
     3421 cggcatgagt tcgccgcgca acatgaccgt gagcggcaaa ccgtgcaggg tgatcgtcag
     3481 ttcgtcgaga acctgatccg gcccggtgta gcgcaactcg ctatcgacgc gcactttcgg
     3541 cgtatgcagg ccgtcgggca ggtcgatgga attcagtagc gtcgtggagc tcacagagac
     3601 gtagcgctcc agcgtctgaa tctgtgtcca actgtggccg atgcagcggt cgtgggcgtc
     3661 gaagatgccg atttgctggc gctcgtagag caggttgtcc ccggcctgag gttcgacgcg
     3721 cggggggtcg ccggctgtcc agcgcggcag tatgtcgcgc acgaacaacg aagtgttgcc
     3781 cgcgagcatg gccagcacta cgccaattcc gatccagcgg ttcagcattc gggcctcatg
     3841 ctcgcaacca gcccggcggg ccattcccgc cgccagcctg cacgaattct acggatgccc
     3901 aagtaagcgg gtcaattctg agcgctgcgc cagccggcag gtgcgaaaat cccccaactt
     3961 cgctataact ccacccccat ggacctgacc atcctggtcg tattctgcct cgtttacatg
     4021 ggaatgatcc tcggccgctt tcccggcctg gcgatcgatc gcaccggcgt ggccctgctg
     4081 ggcgccatcg ccctggtcgc caccggccgg ctgacgcccg aagccgcctg gaacgcgatc
     4141 gacgtgccca ccataacgct gctgctgagc ttgatggtgg tttcggctca gttccgcctg
     4201 ggaggtttct attcggtggt cgcccaacgc ctgtcgaccg cgcaagtctc acccagcgta
     4261 ctgctgcttc tgctgatcgg tgtgagcgca ctgctgtcgg ccgtgctggc gaacgacatc
     4321 gtctgcctgg ccttcacccc cctgctcatc gagggctgtg tgcggcgcgg attgaagccg
     4381 gtcccgttcc tgctggcgct ggcgtgcgcg tcaaacgtcg gatcggccgc cacgctgatc
     4441 ggtaatccgc agaacatgtt gattggccag aaactgaacc tgtccttctc cggctatttg
     4501 cttgacggcg tcttgcccgc ggcgctgggg tgcgtggcca tttggctgct gatttgctgg
     4561 ttggtgcacg gccagtggcg gcagcggccg ggcacgatcg atatccccgc gccgcacttt
     4621 gacctctggc agaccgccaa aggcagcgtg gtgctggccg gtttgatggc ggcgtttctc
     4681 ttcgcgccct ggccgcgtga agtcgtggcg ctggccgcgg ccggcctgct gctgatgagc
     4741 cgctacctgc acacgcgccg catgctggcg caggtggatt ggcagctcct ggtgctgttt
     4801 gccggcttgt tcgtcgtcaa tcacgccctg gcggcctccg gcatgctggc gcagggcatg
     4861 gaacggctgc acgcggccgg cgtcgcggtc gagcaacccg tgtggctctt tggcacgacg
     4921 gtggtgctct cgaacctcgt ctcgaacgtg ccggccgtca tgctcttgct gccggcggcg
     4981 acgcaccctc aagccggggc aatcctggcc ctggctagca cgttggccgg caatctcttc
     5041 attgtgggca gcattgccaa catcatcgtg atcgaccagg ccgagcgcct tggcattcgc
     5101 atcacgtggc gtgagcacgc acgcgtcggg gtacccgtaa cgcttgccac gctggccatc
     5161 gccggcgcgt ggctgtattt ccgcgccggc gcacagtgtt aagctgtcgg ctggcagtta
     5221 tcagctttcg cctcactcgc atcagggagg tcgcacctac ggcgtgataa agtgataaag
     5281 tgatagagtg ataaggggtt tcgcggacca cttttatcac cctatcactt tatcacctta
     5341 tcacgttatc gcaggcgcgg cttgggtctc ggttgacgtt tgggcggtag ccggcgccgg
     5401 ccacgcgcta gggcttgtta cgattgaagt caaacggaaa gcgcggctgg cgggcggccg
     5461 ggtcatccag gccgagggcc aggcgtgccg cctcgggtag cgattcgagc gtccagggcg
     5521 gatcccacac caattccacc ttcacatctg tcacgccgtg taccagtctg actcggtttt
     5581 ccacttcggc cggcatcgat ccggctaccg ggcacagcgg cgatgtcagc gtcatgcgaa
     5641 tatggaccgc cccctgctcg cttacgtcca cgccataaat caggccgagg tcgtagatgt
     5701 tcagtggaat ttccggatca aagattgtcc gcagcgcccg gatggtttcc gcctctatgc
     5761 gtttgcgatc cagttcgtcc atcaggtcgt cccacatcta tgcgtttgca cattaccttc
     5821 ggttatcaca gttcgtccga tttctatgcg tttgcaaatc gcgttcgggg ggtcacagtt
     5881 cgtccgactc ggtcgacact acttcgccgc ggttctccaa tgccgcatcc agcgtgtgcc
     5941 aagccagcgt ggcgcatttc acacgcgccg ggtacttgcg cacgcccgaa aagacttcca
     6001 gcttgcccag tcccggccca cccaccggtc ccggcccacc cagcggctcc tgcggctgcc
     6061 ccgtaaccag gccgtggaac cgcctgaaga tcgccctcgc ctcggccagc gttttgccct
     6121 tcagcgtctc cgtcatcagc gaggctgagg ccgtcgagat cgcacagccg ctgccctcga
     6181 aagtcacttc ctttaccagg tcgctgtcca tcttcaggaa cacggtgaca tggtcgccgc
     6241 acagcgggtt atagccctcg gccatgcggt ccgcctgcgg cagcgtccgc cggttccggg
     6301 gccggcggta atgatccagg atcaactgct gatacagttc gtccagttcg ctcatgaaaa
     6361 gaccttcacg acctgccgta tccccgcgac caggacgtca atctcctcat gagtattgta
     6421 gaaggccagc gcggcgcggc aggtcgcggg cacgccgaaa tgttgcatta ccggttgggc
     6481 gcagtgatgc ccgctgcgaa ttgcgatgcc cagcgtatcc agcaccgtgc cgacgtcgtg
     6541 cggatggatg tctttgagca cgaatgacag aatgcccgcc ttctcactag cggtgccgat
     6601 cagccgcact tgcgggattt cgcccagggc tgcggccccg tatgcgagca aatcgcgctc
     6661 gtaagccata atggcgtccc ggcccaggcg ttcgaggtat tccacggccg cgcccagccc
     6721 gatcgcgccg gcgatgttcg gtgtgccggc ctcgaagcgg gccggcggct cgcgaaaggt
     6781 tgtcttttcg aatgtaacgg cgctgatcat ctcgccgccg ccctggtaag gcggcatgga
     6841 ctcgagcagc tcatatcgcc catacagcac gcccacgccc gtcggtccga aagtcttgtg
     6901 actcgagaac acgtagaaat cacagtccag ggcgcgcacg tccaccggca tatgcggcac
     6961 agcctgggcg ccgtccacga gaaccaccgc accggcgcta tgcgcccggg cgatcacgtg
     7021 ctcgaccggg ttgactgtcc ccagtgcatt cgagacgtgc gtcactgcga ccaggcgcgt
     7081 gcgcggcccg agcatgtgct catactcttc catacgcagc tcgccgcgat catccatcgg
     7141 gacgacccgc agcacggcgc ccgtctgctc acacagcatc tgccagggca cgatattgga
     7201 gtgatgttcc atctccgaga tcaggatttc gtcgccggcg cgtacctgcg cgcggccgaa
     7261 ggtctgcgcg acgagattga tcgcctcggt ggccccgcgc acgaacacta tttcttcggc
     7321 ctgaccggca ttgatgaaac gctgcacctt gtggcgcgcg gcctcatagg cttcagtggc
     7381 gcgcatgctc agcgcatgca acccgcgatg cacgtttgca ttgtcgcgct cgtagtagtc
     7441 ccgtatacac tcaatcacta cttgtggctt ttgcgtcgtg gccgcattat caagatagac
     7501 cagcggatgg ccgttgacct cctgcagcag cgcgggaaag tcggcccgga tgcggcgcac
     7561 ctccagcggt gcttccgacg gcgtgtcacg taccagtgtg gggatcgtgc tcacgacagc
     7621 gcctcgagga attcgccctg cggcagccgg tccgccagtt cacgctgcag ccgccgccgc
     7681 aaggactcaa tttcgacctg cgccaggctc tcgctggcga aggcatagac cagcaggctg
     7741 cgggcggcgg cctcagacag gccgcgcgag cgcaggtaga acagcgcgtc gcgatcgatc
     7801 tgcccgatgg tcgccccgtg tgtgcacttc acgtcatccg cgtggatctc gagctgcggc
     7861 ttgctgtcca cttgggcctc gcccgagagc atcaggttct tgttggtctg cttggcgtcc
     7921 gtctgctgtg ccccgcggtg caccacaatg cgcccggtga agacggcccg cgcccgtcct
     7981 tccagaatac ccttaaacac ttcgcggctg tcgcagtgcg gcgcgacgtg ttcgacgcgc
     8041 agatgattgt ccacatgctg gtcgccaccc accaggtata gacccagcag ccggcaatgg
     8101 ccgcccgccc cggccaggac actgttgata ttgtgccgga ccagtccgcc gccgagcgta
     8161 atgcaatgcg agagaacttc gctgtcgcgc ttctgatgaa tttgcactgt tccgatgtga
     8221 tacgtgccaa gcgcctcgcg cgccagcttg tagtgccgca actgggcccg ttcgccgacg
     8281 accagttccg tgaccgcgtt cgtaaaagac gtcccctcgc ccaggctgat gtagctttcc
     8341 acgatcgaag cctgggcttc gtcatccacg agcacgagcg tacgcgggtg gaccatgctc
     8401 ggcgcctcgc ccggcacact gatgtgcaca atgtgcagcg gccgctcggc catgtgccgc
     8461 cgcggaatcc ggacgtaagc cccatccgcc ataaatgcgg tgttcagcgc cacgaagctg
     8521 caatccatgt agtcggcgtg ttgcgccaga tagtcttcaa gttcgccgtc acccgtggcc
     8581 agcgcgctcc ccaggctgcc gcagcggacg ccgttttcgg ccgacaatgt cgagaggtcc
     8641 ggcgcgtacc ggccgttcac gagaatcatt tgctcgccgc ccacatccca cggcaaagca
     8701 cgcagcatcg ctcgttgggc cggcaagtcg caggccgcag ccggcctgaa tggcgtgcgc
     8761 tccagggcgc taacgtttgt aaagcgccaa tcctcatcgc gcgtggtcgg aaagccacgt
     8821 tccaggaagc gcgccagcgc cgcctgccgc agctcccgca gccacgggcg gccgccgtca
     8881 agccgacctt cga
//
LOCUS       DAOSYS010000153         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6829_length_8758_cov_4.841434, whole genome shotgun sequence.
ACCESSION   DAOSYS010000153 DAOSYS010000000
VERSION     DAOSYS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6829_length_8758_cov_4.841434"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            450..2474
                     /locus_tag="PLU99_10185"
     CDS             450..2474
                     /locus_tag="PLU99_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17466.1"
                     /translation="MKKLRPTAPAPSRTAVSSSACRAAKLGSPGSPRYAPVLLIAAGL
                     AIYANSFNTSFIFDDVSITDNNHLHTLWPLWRTIFAPADLADSGRPVVTLTLLLNYAA
                     GGLDVGGYRAVNIAIHLLASLVLYGVIRRTLLGPALRDRFGPASGALALVVALLWLVH
                     PLQTECVCYTSQRSESLMGLFYLLTLYCAIREMDSAHHRLWRAAAIVACALGMASKEV
                     MVTAPVTVFLYDWAFRAQPFNQVLRRRYGLYLGLAATWAVLAALMLGAPRAGSVGWAL
                     GASTLDYALSQCVMIATYLRLALWPHPLVLDYGPARPTTLLETWPYLIALTALLLLVG
                     WTCWRRPRVGFAAAWVFIILAPTSSIIPIATEVGAERRMYLPLAGVVVLVVFGIHELL
                     RRWSRPRAAPRASRTETGTASFPRGPAFALVALATVALGATTIKRNADYRSPLAMWKT
                     VVHARPQNARAHRGLANALVAEGRIAEAVPHFQEGIRLDPQDAKGLSNLGLALGKLNR
                     PAEAVVEHRRAVQLAPQSAEVRSRLAYALTRTGQLEEALAEFAAALRLDPTLIDARAN
                     MGGVLAQLGRPAEAAEQYEQVLRQDPNYLAARVSLGLICLQRNETDKALEHLQVAARL
                     KPDPELMVLLGQALEAAGRTSEAIEQYRRALNVAPGHAQAQARLSALGGT"
     gene            2893..3480
                     /locus_tag="PLU99_10190"
     CDS             2893..3480
                     /locus_tag="PLU99_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17467.1"
                     /translation="MSGFGGVTQAATGQPGFGTGAGMFGTLAPVTVRGDLTQPGIAGT
                     GLGPVNTGGAGLPLITTSSRAQLANTGYGATAQAFGFGPYVGTAANVNPGLQPTVVHP
                     AWTVAPPSQATYGSLGPPYGVMAGTAGAPAAGSVGTFRPAPIALGRYTPDDTAVRPVS
                     PAQTTVRPQTYPRPWRSYWFKLGQGGLGSSPERQP"
     gene            complement(3745..5175)
                     /locus_tag="PLU99_10195"
     CDS             complement(3745..5175)
                     /locus_tag="PLU99_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOO17468.1"
                     /translation="MSAKPFTGRVYVVATKRAYLNPLETVEWFNSDPVFARDVQNWKP
                     GETLLINSENCLAYPSKPVDLPANIYRVQAVMGLNDWAQHPIDASGNGCSEIAVFKHP
                     VAQSPTIRLVINHKIPQPPVAHGDGIEYVRVKSELLSKFHRRDVYLEAVLALPPSYSG
                     EKSETDRRFPAVYQIPGFDGRVQFANPAPFASLLAMNGLDAVVVALVASCPTGHHVFA
                     DSANNGPWGKALVTEFIPYLEAHYRLIPDAAARYLTGHSSGGWSSLWLQVTYPQVFGG
                     VWSTSPDPVDFNSFMQINIYDPPESVFSNADGSPRMLSRPGFFGHIGWRALSDLEQVM
                     GRGGQLGSFEAVFSPRGPDCKPARLWDRATGVVDRDVARAWRKYDICHVLESNWSTLG
                     PSLAGKLHVYCGDRDDFFLEQPFLRLRETLAKLGSDAHLELVSGATHILPTWVFNEIA
                     GEMANQFAALQQTTKEPGQPAGALLD"
     gene            complement(5534..7489)
                     /locus_tag="PLU99_10200"
     CDS             complement(5534..7489)
                     /locus_tag="PLU99_10200"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOO17469.1"
                     /translation="MRTRRLAGWSLVIGVLAVGSVSPAQDEVLIDRGAPGLLLSFYEL
                     EQSPQFMPELAAGQLPNLTRIVRTLDLDSNRGDFAAVNSPFLTEVTGYIHTTSPANYA
                     FRLISDDGARLWIDDHLVIDHDGLHGPEPKDGTVDLTAGTHKLRISHFDAGGGQRLAL
                     LWKPPDAEDYAAVPAGALTHDAGAVSKSAAGKKRIIPPLRRGLPGDGTPVARTHPSFQ
                     PPPAAGQSATPSGPVRDGQYVRLPAPVWVPDCLAVAPVSPTYSIALDRDGDQAASQLQ
                     VSSDSGEWKRIFVDEYGEIRQGCVFRFSAPSGPAFSKHEGTVFEMLGVRAMSNGFEIE
                     FTKPLDARCGWEPDSYVVEQWPFVFAEGRGPRRDGSSTPVKSASVSPDRRKVFLEVEG
                     LQPSSVVYIRLLPPCFAEDGQRPWSTEAWYTLSRLPQDRTGQVLPPPTKAPQNILTDA
                     ERQAGWKLLFDGKTPAGWHGWKKSEFPAGWEVIDGCLVRTGRGGDIVTDEEFENFELA
                     LEWRISPGGNSGIFFRADESLRWAWETAPEMQVLDNAEHADGRSPLTSAGSNYALHAP
                     TRDVTRPVGLFNEVRILVNGAHVEHWLNGEKIVEYELGSPQWQELVAASKFKDMPKYG
                     RTAKGRIVLQDHGDKVWYRNIKIRQLP"
     gene            complement(7609..>8758)
                     /locus_tag="PLU99_10205"
     CDS             complement(7609..>8758)
                     /locus_tag="PLU99_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17470.1"
                     /translation="DAAKAAEDVIVELAQSDEDRDRRAGPVLAALKDSSGIAAGRLLR
                     VLGRLQGPAALAAIRAAVQSSDDIAADAAFRALADWPDAEVLDDLLQLAGSQNEIRHA
                     LALRGYLRLVRLPSERPAEQTLEMLKYAMSLARNADEQKLVLAAMGEVPHLAALTEVQ
                     NWLRKDELRDEAAAAVVSVARGLALWYPDEARAALERIVAAGVAEGPTNQARQALQAI
                     EEHAGAIGVWAVSVPCLAEGETYADVFAHEFEPETGRLAEIEWTPLTATRTDNPWVFD
                     LNKVGKCSNCCVYARTAIWSENEQPARLELGSDDGVKVWLNGQLVHSNAASRGVTPGE
                     DKVEIRLQRGWNPLLLKIVQAGGNWGFTCAVRDPAGQPVPDLKFDADR"
BASE COUNT         1477 a         2919 c         2761 g         1601 t
ORIGIN      
        1 cacctttcac ctctctcctg ctctcgcgca gcgagttatc ggcactttgc caaattcgag
       61 cgcgcatacc ttggcaaact ggcaacctgc ccctggcgca agccaaacac cggttttatc
      121 ggtcaaaaca gcaacccctt ggcaatctgg caaactgcct atccagccca aacatgaccg
      181 cgccccgcgc actcactatg cgccattcga aatgtgcccc tccttacctg tcacctatca
      241 tccctctccg cgctctctca ccggctcgcc tctcacctat caactctctc cgcgcagcgg
      301 cgcctcaggt tatcaaaatc gggtgcacaa aaaccatgag tatctgatca tctgcccccc
      361 gtgcccgcag ccacacgcct ttttatcaga ccgcttgagc acctggggta gcaccgcgcg
      421 caggcgtgcc ggctcgtata atgctcggcg tgaagaagct cagaccgacc gcgccggcgc
      481 cgtcccgaac cgctgtttcc agcagcgcct gccgcgctgc caagctcggc tcgcctgggt
      541 cgccccggta tgcgccggtc ctgctgatcg ccgccggcct cgcgatttac gccaacagct
      601 tcaacacctc cttcatcttc gacgacgtca gcatcaccga caacaaccac ctgcacacgc
      661 tctggccgct ttggcggaca atcttcgctc cggcggacct ggccgattcc ggccgcccgg
      721 tcgttacgct gacgctcctg ctcaactacg ccgccggcgg cctggacgta ggcggctacc
      781 gcgccgtcaa catcgccata cacctgctgg cgagcctggt tctgtacggg gttatccgcc
      841 gcacgctgct ggggccggcg ttgcgcgacc gctttgggcc ggcttccggc gcgctggcgc
      901 tggtggtcgc tcttttgtgg ctcgtgcatc cgctgcagac cgagtgcgtc tgctatacgt
      961 cgcagcgcag cgaatcgttg atgggcttgt tttacctgct cactctctac tgcgccatac
     1021 gcgaaatgga ttcagcccac caccggctgt ggcgggccgc ggccatcgtc gcctgcgccc
     1081 tgggcatggc ctccaaggag gtcatggtca ccgcccccgt tacggtgttc ctctacgact
     1141 gggcgtttcg cgcccagccg ttcaaccagg tgttgcgccg gcgctacggg ctgtacctcg
     1201 gtttggctgc cacttgggcg gtcctggcgg ccctgatgct gggcgcaccg cgcgccgggt
     1261 cagtcggctg ggcgctgggc gccagcacgc tcgattacgc cctgagccag tgcgtcatga
     1321 ttgccactta cctgcgcctg gcgctgtggc cgcatccgtt ggtgctggat tatggcccgg
     1381 cccgcccgac gaccctgttg gagacttggc cttatctcat cgcgctgacg gcgctgctgt
     1441 tgctggttgg ctggacgtgc tggcgccgcc cgcgtgtggg cttcgcggcg gcctgggtgt
     1501 tcataatcct ggcgccaacg tcgagcatca tcccgatcgc gaccgaggtt ggggcggagc
     1561 gtcgcatgta cctgccgctg gccggcgttg tcgtgctggt cgtattcggc atccacgagc
     1621 tgctgcggcg ctggagccgt ccgcgcgcgg caccacgcgc ttcgcgaacc gaaaccggca
     1681 cggcgagttt cccgcgcggc ccggcattcg cgctggtggc gctggcgact gttgcgctgg
     1741 gcgcgaccac gatcaagcgc aacgccgatt accgcagccc gctggcgatg tggaagaccg
     1801 tcgttcatgc ccggccgcag aacgcccgcg cccaccgcgg cctggccaac gcgctggtcg
     1861 ctgagggccg catagccgaa gccgtaccac acttccagga gggcattcgg cttgacccgc
     1921 aggacgcgaa ggggctgagc aatctggggc tcgcacttgg caagctcaat cgtccggcgg
     1981 aagcagtcgt ggagcaccgg cgggccgtgc aactggcgcc gcaaagcgcc gaagtgcgca
     2041 gccggctggc gtatgctttg acgcgcaccg gacaactgga ggaagcgctg gccgagttcg
     2101 cggcagcgct gcgcttggac cccacgctga tcgatgctcg tgcaaacatg ggcggagtgc
     2161 tagcccaact gggacggccc gcggaggccg ccgagcagta cgagcaggtg ctgcgccagg
     2221 atccgaatta cctggcggcc cgtgtgagcc tgggcctgat ctgcctgcag cgcaacgaaa
     2281 cggacaaagc attggagcac ctccaggtgg ccgcccggct caagccggac cccgagttaa
     2341 tggtgcttct gggacaggct ctcgaagctg cggggcgaac gtcagaagcc atcgagcagt
     2401 accgccgcgc actaaacgtg gcccctgggc acgcgcaggc acaggcccgg ctgagcgcgt
     2461 tgggcggcac gtaatgtccg acttcgccgg cacgttcagt cgcacataaa cgcagtttca
     2521 gccgacttgg cggtgattta tatccggctc ggagctctaa tcgccggccc gcgtatgccg
     2581 cgcctatgct gatagtggtg gactatggcg aaaacactcc cgtcgctgac gaagctactc
     2641 ctgtcgttgc tgttcttgct cggagttcaa ggggctgaag cgcaggtaag cgcgcagcct
     2701 gccgctccgc aacgtggacc cagccaacag cctgatccgg gaacatcccc cggcggtgga
     2761 ttgccaggga caccgtcggg cggtctggtt cgccccgcac cagcgccgac cggccttggc
     2821 ggaaacgtga atggcgttgg cccagccggc aacgcgctgc tcgctcccgc ccctcaggcg
     2881 attggcgtag gcatgtccgg ctttggcggc gtcacgcagg ctgcaaccgg tcagcccggc
     2941 tttggaaccg gcgcgggcat gttcggcaca cttgccccgg tgaccgtccg cggcgatctg
     3001 acccaacccg gcatcgcggg caccggtctg gggcctgtga acaccggcgg ggcgggcttg
     3061 ccactgatca caaccagcag cagggcccaa cttgcaaaca cgggctacgg cgccacggct
     3121 caggccttcg gtttcgggcc atacgtcgga accgcggcga atgtcaaccc gggactgcag
     3181 cccactgtcg tccacccggc ctggacggtc gccccgccca gccaggcaac ctatggcagc
     3241 ttggggccgc cttacggcgt aatggcggga accgcaggcg cgcccgcggc cggctctgtc
     3301 ggcactttcc ggcctgctcc gatcgccctg gggcgctaca ctccagacga caccgctgta
     3361 cgcccggtca gccccgcaca gaccaccgtt cgcccacaga cctacccgcg gccctggaga
     3421 tcctactggt tcaagctggg ccaaggtggc ttgggttcat ctcccgaacg ccagccgtag
     3481 agcagcgccg gggcgtgccg caccgggttg atgggacagg gcatcgaagg gatcgagaga
     3541 tcgagggatc aagaagcgcc gcaagatttg ctcaaccagg ccgaagcggt cagagccggg
     3601 gtcgccattg gtttaagaat tcaaaaaagg tggcccgcgt ggccagagac gcgggcctgg
     3661 tctgattgcg ggtggcgggg gccgccgccc gcggagaagg atacaccggg agacgccccg
     3721 aagagggcca tccctgtcaa gaccttagtc taacagcgct ccggcgggtt ggcccggttc
     3781 ttttgttgtt tgttgtagcg cggcaaattg gttggccatc tcgcccgcga tttcattaaa
     3841 cacccacgtc ggaaggatgt gagtggcgcc gctaactaac tccaggtggg cgtcgctgcc
     3901 cagtttggcc aatgtttcgc gcagcctcag gaacggctgc tcgaggaaga aatcatcacg
     3961 atcgccacaa tacacatgta gcttcccggc aagcgacggc ccgagcgtgc tccaatttga
     4021 ctccagtacg tggcagatgt cgtatttgcg ccacgcgcga gccacgtcgc ggtcaaccac
     4081 accggtcgcg cgatcccaca gccgggccgg tttgcaatcc ggcccgcgcg ggctgaaaac
     4141 cgcctcgaac gagccaagct gaccgccccg ccccattacc tgctccaagt ccgacaaagc
     4201 ccgccagccg atatggccga aaaagccggg ccgcgacagc atccgcggcg agccatccgc
     4261 gttagagaaa acactttccg gcggatcgta aatgttgatc tgcatgaagg agttaaagtc
     4321 gaccggatcg ggcgatgtgg accaaacgcc gccaaatacc tgcggatacg tgacttgcag
     4381 ccacaaactg ctccatccgc ccgacgaatg gccggtcagg tagcgagccg cagcgtcggg
     4441 aataagccga tagtgcgcct caagatatgg aatgaactcg gtcacgagcg ctttccccca
     4501 cggcccgttg ttggcggagt ccgcgaacac gtgatgtccg gtcggacagc tcgcgaccag
     4561 ggcaactaca acggcgtcaa ggccattcat ggctaacaga gaggcgaacg gcgccgggtt
     4621 ggcaaactga acgcgtccgt cgaagccggg aatctggtaa accgcaggga agcgacggtc
     4681 cgtctccgat ttctcgccgc tgtacgaagg gggcaacgcc aacaccgctt ccaggtaaac
     4741 gtcgcgacgg tgaaacttgc tcagcaattc acttttcacc cgcacgtatt cgatgccgtc
     4801 cccatgcgcg accggcggct gcggtatttt gtggttgatg accaggcgta tcgtgggcga
     4861 ctgggcgacg ggatgcttga agaccgcaat ttcgctgcag ccattgccgg acgcgtcgat
     4921 ggggtgctgg gcccaatcgt tcaagcccat gacggcctgc acgcggtaga tgttcgccgg
     4981 caggtcgacc ggctttgacg ggtaagccag gcagttttcg gagttgatga gcagtgtttc
     5041 gccgggtttc cagttctgca cgtcgcgtgc gaaaaccggg tctgagttga accactctac
     5101 tgtctccaac gggttcaggt aggctcgttt tgtcgcaacc acgtagaccc ggccggtgaa
     5161 cggcttcgcg ctcaccgagg cgtcaaacgt gatttcgaac tgcgggccgg tgttacgggt
     5221 ggtcgtcggt ggccgggtgg gcgcttgccg ggcgggcgcc gttgatgttt gtgaaagacc
     5281 aggcgcggca gtgaacagtg cgaggagaat gagggctgcg ctgcggagtc tgttctggga
     5341 ggcgagcgct gcgaggtcaa caaggcccgc gttaccgagc ctttcccggg aggcatgccc
     5401 gcgcttacag cttgggcatg gcacccgcgc tcgatccgtg tcccgtcctt gatccctcga
     5461 tccctcgatc ccttgatccc tcgatccctc gataccttga tccctcatca tgttccttca
     5521 gccacttttt ttgttacggc aactgccgaa tcttgatgtt ccgataccac accttgtcgc
     5581 cgtggtcttg aaggacgatg cgacccttgg cggtgcggcc gtacttgggc atatccttga
     5641 atttgctggc ggcgacgagc tcttgccatt gcgggctgcc cagttcgtat tcgacgatct
     5701 tctcgccatt gagccagtgt tcgacatgcg cgccattcac caggatgcgg acttcgttga
     5761 acagtccgac gggccgcgtc acgtcgcggg ttggggcgtg gagggcgtaa tttgagccgg
     5821 cggaggtcag cgggctgcgc ccatcggcgt gctcggcgtt gtcgagcacc tgcatctcgg
     5881 gagcggtttc ccaggcccag cgcaggcttt cgtcggcgcg gaagaaaatg ccgctgttgc
     5941 cgccagggct gatgcgccat tcgagcgcga gctcgaaatt ctcgaattcc tcgtcagtga
     6001 cgatgtcacc gccgcgcccg gtgcgcacca ggcagccgtc gatgacttcc caaccggcgg
     6061 ggaactcgct ctttttccag ccgtgccagc cggcgggggt cttgccgtcg aacagcagtt
     6121 tccagccggc ttggcgttcg gcgtcggtga gaatgttctg cggcgccttg gtcggtggcg
     6181 gtagcacctg gccggtgcgg tcctgcggca gccggctaag cgtgtaccag gcttcggtgc
     6241 tccaggggcg ttggccgtcc tcggcgaagc agggcggcag caggcgaatg tacactacgc
     6301 ttgagggttg caggccttcg acttccaaaa agacctttct gcggtccggc gagacgcttg
     6361 cggacttgac cggcgtagat gaaccatcgc gcctcgggcc gcgtccttcc gcgaaaacga
     6421 agggccattg ctcgacgacg tacgaatccg gttcccagcc gcagcgcgcg tccagaggtt
     6481 tggtgaactc gatctcgaag ccattactca tggcgcgcac gccgagcatc tcgaatactg
     6541 ttccctcgtg cttcgaaaac gccgggccag atggggctga gaagcggaaa acacatcctt
     6601 gccggatttc gccatactca tcaacgaaaa tgcgtttcca ctcgcccgag tccgacgaaa
     6661 cctgtagctg gcttgccgct tggtctccgt cacggtcgag cgcaatcgaa taagtcggcg
     6721 aaaccggggc aacggcgaga caatccggca cccacactgg cgcgggcagt cgcacgtact
     6781 ggccatcacg cacgggtccg gatggcgtgg ctgattgacc cgcggccggg ggcggctgga
     6841 aagacgggtg cgtgcgggcc acgggcgtgc cgtcgccggg cagcccgcgc cgcaacggtg
     6901 gaatgatgcg cttcttgccg gcggctgatt tggagacggc gcccgcatcg tgcgtcagcg
     6961 cgcccgcagg aacggccgca taatcctcgg catccggcgg cttccatagc agggccagtc
     7021 gctgaccgcc gccggcgtcg aaatggctga tgcgcagctt gtgcgtgccg gccgttaagt
     7081 cgacggtgcc gtccttgggc tcggggccgt gcaagccatc gtggtcgatc acgaggtggt
     7141 catctatcca gagccgggcg ccgtcgtcac tgatgagacg gaaggcgtaa ttagccgggc
     7201 tcgtcgtgtg gatgtagccc gtcacctccg tcaggaatgg gctattcaca gcggcgaaat
     7261 ccccgcggtt gctatccaag tcgagcgtgc gaacgatgcg cgtcaggttc ggaagctggc
     7321 cggcggcgag ctccggcatg aattgcggcg attgttccag ctcatagaag ctcagcagca
     7381 ggccgggcgc cccgcggtcg atcagcactt catcctgcgc cggcgagaca gaaccgacag
     7441 cgagtacacc aatgaccagc gaccaccccg ccaaacgtcg cgtgcgcatg ttggtcttcc
     7501 tttccgctat tcagcaactt gtagccctgt gccaccggcg cctggcctat gttttcggcg
     7561 gcgagacgcc ggcggcacaa tggccacgat gtccgtgacg cccagccgct agcggtcggc
     7621 gtcgaacttc aggtcgggga ctggctgacc ggccgggtcg cgaaccgcgc aggtgaagcc
     7681 ccagtttccg ccggcctgca cgatcttcag cagcagcgga ttccagccgc gctgcagcct
     7741 tatctcaacc ttgtcctcgc cgggcgtgac gccgcgcgag gcggcattgc tgtgtacaag
     7801 ctgcccgttc agccagacct tcacgccgtc atcgctgccc agttccaggc gtgccggctg
     7861 ctcgttctcc gaccagatcg cggttcgggc atagacgcag cagttactgc acttgccgac
     7921 cttgttcaga tcgaaaaccc aggggttatc cgttcgcgtc gcggtcagcg gcgtccactc
     7981 gatctcggcc aggcggcctg tctcgggttc aaactcgtgt gcgaacacat ccgcgtaagt
     8041 ctcgccttcg gccaagcacg gcacgcttac ggcccaaact ccaatggcgc cggcgtgctc
     8101 ctcaatggcc tgcagcgcct ggcgcgcctg gttcgtgggc ccctccgcta cgcccgcagc
     8161 cactattctt tcgagcgcgg cccgggcctc atccggatac cacagcgcga ggccccgcgc
     8221 cacgctaaca acagcggcgg cggcttcgtc gcgcaactcg tctttgcgca gccagttctg
     8281 cacttccgtc aacgccgcca aatgcggcac ttcgcccatc gccgctagaa ccagcttctg
     8341 ctcgtccgcg ttgcgcgcca gtgacatggc gtacttcaac atctcgagcg tctgctcggc
     8401 cggccgctcg ctcggcagac gcaccagccg caggtagccg cgcagggcca gcgcgtgccg
     8461 aatctcgttc tggctgccgg caagctgcaa gaggtcgtcc agcacttcgg cgtccggcca
     8521 gtcggctagt gctcggaacg ccgcgtcagc agcgatatcg tcgctcgact ggaccgcggc
     8581 ccgaatcgcc gccagcgccg ccggcccttg cagccggccc agcacgcgca gcaggcggcc
     8641 ggccgcaatc ccgctggagt ccttcagggc cgccaggacc ggcccggccc gccggtcgcg
     8701 atcctcatcg ctctgggcta attcgacgat cacatcctct gcggctttgg cggcgtcc
//
LOCUS       DAOSYS010000154         8695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6902_length_8695_cov_6.061574, whole genome shotgun sequence.
ACCESSION   DAOSYS010000154 DAOSYS010000000
VERSION     DAOSYS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6902_length_8695_cov_6.061574"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3676
                     /locus_tag="PLU99_10210"
     CDS             <1..3676
                     /locus_tag="PLU99_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HOO17471.1"
                     /translation="ICEAAHHAHTQGVIHRDLKPQNILVDEAAEPHILDFGLAKAARQ
                     ADPEGSTLQGLSTAGQLLGTVAYMSPEQTVGSQDVDVRSDVYSLGVVFYEALLGELPY
                     AVEGPLGEVLRRILEDEPRRPRDLRNGSRFGHLLNDELETILLKALEKDPARRYQTAG
                     ELGRDLRRLLDGEPIEAKPAGGLYVFRKMLRRYRLQAGIAFAAFTTLVVCLVLFATLW
                     RKASEAAARAAQQEAIAWQHEAAAQRAREEADQGRQELEQALIEQTLQRGRLAEMRGD
                     LVLARDHYWDAYDHGSPAAQWALRQYYQGSGDVGADVLFCAREGPVSISRDGTLAAFC
                     EFPESITVRRLADAQTLRWLRAPGRVEVLNVYADGTVCAGGAGWARLWRPDSFSPALA
                     HELPDDFVLRAIHLVNSDDLLLVGDKSIRLARSGQSGPDERLNLIGILVGNTDCLPEQ
                     NLLAAATSAGVELVAIDEEGGLRSEVVSLRDVGSSVRHVVFSGRYLFAATAEALYRTA
                     TSGAALGVWQSAPEVPLTWRTFGEWDLLDLNPAARALVVGMRDGRILLFRRGRLEHTW
                     QVSAGLLGLRLTSDAASVLTLDKAGTVTRWEPPAPREDRRILAQRPAGWAVADDSSAV
                     LVADEEQRIYAYAPRRGSECEAIPIRRGLRRHLPRSDHLALGVSRDAERVVICADGMV
                     RLRDRASGAAYNATWSDESAAVLKAVALTGDGQYLAFYSQSEAGDRQHVTFYRWQPRV
                     QTSRSIDLATLLPACGPPVEFVGSAIRELAFLPGTARLAVVRSNGELVLLDAEGDGTA
                     GPASAGTSPVPPPAAWMILESPAFAMSFDGRGEQLALACDDGFIRIVALADAKLKGRI
                     RPPEPIRSLAFNPGGRVLLTLDDQGKVSILDLATGDSVLHVPLTTGQAGLLATWIGGN
                     DAVLLGQPGGLYEHDFARVDALIAQNVSYARQRAVARQLAEGDVERAWERSDALELVD
                     PWRAAGMRLLVLETALRRRSQVIPSAWLAVTASATEAAVWLRLGHAAYEGERFAEAKT
                     WLERSAELSGTQLDASSERRIAQCRYLAGEYLAAAEAFAQLSARPDLDSTLLPAVQLE
                     QVAALVLGQETAKARSVAARIGGLGRAGVRLDPGATGAARQIARTLTGLESEIPTMPI
                     GLEADGPVRLLTQLAEANLRSMDLRFHDDAQFFAGELARASGQTQRAIAAYQRCIDLA
                     RDEWPSGWARYRLRQLSDGI"
     gene            3695..4954
                     /gene="purD"
                     /locus_tag="PLU99_10215"
     CDS             3695..4954
                     /gene="purD"
                     /locus_tag="PLU99_10215"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOO17472.1"
                     /translation="MNVLVIGSGGREHALCYRLARSPRLDKLFILPGNAGTAALGTNI
                     PGDAADIRLALEVARRESIDLTIVGPEDPLAAGIVDAFEEAGLRIFGPTAAAARLESD
                     KSFAKTLMRQQAVPTADARIFDNYDQARQYIATRDEPVVIKAAGLAKGKGVIVCEDPA
                     DGILAAERMMVNRIFGDAGARIIVEERLNGREASLLAIVDGQTIFVLEPAQDYKRLGA
                     GDTGPNTGGMGSFSPTSVLDDELLAEIQRQILVPTLDGLTREGIHYCGVLYAGLMLTP
                     AGPKVLEFNCRFGDPETQAILMRLQSDLLELLDAAVSGHLEDAAVRWDPRHALCVVMA
                     SQGYPDKYRTGLPITGLPEDALRDDLAIFHAGTRIQDGQVVTSGGRVLGVTALGGSLS
                     DARRVAYEAVGRIHFEGAYFRPDLGQE"
     gene            complement(4970..6820)
                     /locus_tag="PLU99_10220"
     CDS             complement(4970..6820)
                     /locus_tag="PLU99_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO17473.1"
                     /translation="MSLRFCETLATLIICNCAAGALAQAAAEPALPTFVTVSAEVGLP
                     YPMPDDAIVGLVDIDLDGYVDVVTRNRVGVNNGRNRFEAPLVLRGTFISFGDFDNDGD
                     LDAFSTEWRKISVKLDPEDTKTGREKAERARQSRTKRGPAEPQKEEPDPTVEKVKAEH
                     WLLRNDGDGTFTDVLSKSGLTDAPEAQSGPQRDAAINQPTEKLLDVSRAHGFLDYDND
                     GNLDLFVAGFEITDTPKQTRAVPCRLFKGDGTGHFTNVTEAAGLQVEPRAATAVAFCD
                     FDNDGFVDIYVANSRSPNWLWHNQGDGTFKDVAEEAGATGGAGSSRAVAVADYDNDGD
                     FDVFVVNYAHDDPKKSQPRSMLLRNDSSPDGGLRFTDVTAECALAWEPPEGYKAEATW
                     QSAVWADLNNDGWLDLFVTESADDGKNHPHAPLKYGKLDSGYSKLWLGQGGQSFVRVD
                     KERNKLRIEDSAGVAAGDFDNDGWCDLLVGSNTGKKSDKIDWFDPTKTRTTLLRGRGL
                     GKSGAENAGYLNLVLSAADRALNGAVVEITLNNGEKRRAFLDVSCGGLAIKSNQLHFG
                     LGSGTVSKISVTIPGGESVGRFEGSWRNTTLLLDVSRKSPKQARTIEAWQ"
     gene            7069..7905
                     /locus_tag="PLU99_10225"
     CDS             7069..7905
                     /locus_tag="PLU99_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOO17474.1"
                     /translation="MSVARIWLALAALALAGGQSPTDGPQTSPGGPIIPDLPHKDFSG
                     ATACPVVRVCRGDTVIVRMGEEAKRLGLIGVQVPGLADPTGRAAERFLENLLAGEAVY
                     VEFAEASPAPDHFGCIPAYLFRAPDGLFVNLELVRQGYAAVPEAGAFEYRAVFERLQQ
                     RARAAGKGRWGERTTDSQAEAKPGERSPGPDRVPKSEARDGGQSTPASRSSPSPVPGP
                     AAQSGAGGDAADDAVVYITAKGAKYHREGCRHVRKSGRPVSLGEARTRGLTPCSQCKP
                     PE"
     gene            8162..8235
                     /locus_tag="PLU99_10230"
     tRNA            8162..8235
                     /locus_tag="PLU99_10230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8196..8198,aa:Arg,seq:tcg)
     gene            8267..>8695
                     /locus_tag="PLU99_10235"
     CDS             8267..>8695
                     /locus_tag="PLU99_10235"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO17475.1"
                     /translation="MRRPLVAGNWKMNLTLTESRDLTRNVAARLSPEAAAKVEVAVCP
                     PFVYLLPVLEAAKETPVQVGAQNLYFEPKGAFTGEVSAAMIAELGAKYVIVGHSERRH
                     TIGHHEDDRMINLKLRAARGLGLTPILCVGETLLEREAEQT"
BASE COUNT         1435 a         2750 c         2918 g         1592 t
ORIGIN      
        1 catctgcgag gccgcgcatc acgcgcacac ccagggcgtg atccaccgcg acctgaaacc
       61 gcagaacatc ctggtggatg aggccgctga gccgcacata ctggacttcg gcctggccaa
      121 agctgcccgg caggctgacc cggaaggctc gaccctgcaa gggctctcga cggccggcca
      181 gctactcggc acggtggcgt acatgtcgcc cgagcagacc gttggttcgc aagacgtgga
      241 cgtgcgctcc gacgtctact ccctgggcgt ggtcttctat gaagctctac tgggcgagtt
      301 accgtacgcc gtcgaaggcc cattgggcga ggtgctgaga cgaatcctcg aagatgagcc
      361 gcggcggccg cgcgacctgc gaaacggctc gcgttttggg catcttctga acgacgagct
      421 ggaaaccatt ctgctcaagg cgctggagaa ggacccggcg cggcgctacc agacagcggg
      481 cgaattgggg cgcgatttgc ggcggctgct ggacggcgaa ccgatcgaag ccaaaccggc
      541 cggcggattg tacgtatttc gcaagatgct gcgacgttac cggttgcagg ccgggattgc
      601 gtttgcagcg ttcaccacgt tggtcgtgtg cctggtcctg ttcgcgactt tgtggcgcaa
      661 ggccagtgaa gcggccgcgc gggcggcgca gcaggaggcc attgcctggc aacatgaggc
      721 cgcggcgcag cgggcccgcg aagaagccga ccagggacgg caggaactgg agcaggcgct
      781 gattgagcag acgctgcaac gcgggcggct ggctgaaatg cgcggcgacc tggttctggc
      841 gcgcgatcac tactgggacg cgtatgacca cggcagcccc gcggcgcaat gggccctgcg
      901 gcagtactac cagggcagcg gcgacgtggg cgccgacgtg ttgttctgtg cccgcgaagg
      961 ccccgtttcg atctcacgcg acggcacgct ggccgccttc tgcgagttcc ccgagtcaat
     1021 caccgtgcgc cgcctggccg acgcacaaac gctgcgctgg ctgcgcgctc cgggccgcgt
     1081 agaagttctg aacgtgtacg ccgacgggac agtctgcgcc ggtggcgccg gctgggcgcg
     1141 cctctggcgt ccggactcct tcagtccggc gctggcccat gaactgcccg acgatttcgt
     1201 gctccgcgcg atacacttgg tcaatagcga cgacctgttg ctggtcggcg acaagtcaat
     1261 ccgcctggcg cgttctggac aatccggtcc ggacgaacgc ctcaacctga ttggcatcct
     1321 ggtgggaaac actgattgtc tgcccgaaca gaacctcctg gcggccgcga cttcggccgg
     1381 agtcgaactg gtcgcaatcg acgaagaagg cggattgcgt tctgaagtgg tcagtctgcg
     1441 cgacgttggc tcgtctgtgc gccacgtggt cttttccggg cgatacctgt tcgcagccac
     1501 ggctgaggcg ctctaccgca ccgccactag cggcgcggcg ctgggagtct ggcagagcgc
     1561 gccggaagtc ccgctcacct ggaggacctt cggcgagtgg gacctgctgg acctgaatcc
     1621 cgccgcgcga gcgctcgtgg tagggatgcg tgacggccgt attctgctgt tccgccgcgg
     1681 gcgcctcgaa catacgtggc aagtcagcgc gggcctgctg ggactgcgac tgacatccga
     1741 cgcggcgtct gtactgacgc tggacaaggc cggcacggtc acgcgttggg agccgcccgc
     1801 accgcgtgaa gatcgccgga ttctcgcgca gcgcccggcc ggctgggccg tggccgatga
     1861 cagttcggcg gtgctggtgg ctgacgagga gcagcgtatt tatgcctacg ccccgcggcg
     1921 cggctcggag tgtgaggcca ttcccatccg tcgcgggttg cggcggcacc tgcctcgttc
     1981 ggatcatctg gctctgggcg tcagccgcga cgccgagcgc gtggtgatct gcgcggacgg
     2041 tatggtgcgg ttgcgtgatc gcgcctcggg ggcggcctac aacgccactt ggagtgatga
     2101 aagtgccgcg gttctcaagg cggtcgctct gaccggcgat gggcaatacc tggcgttcta
     2161 ctcgcagagc gaagcgggcg atcggcagca cgttaccttt tatcgctggc agccgcgcgt
     2221 acaaacgagc cgcagtattg atctggcgac gctgttgccg gcctgcgggc cgcccgtgga
     2281 attcgtgggg agcgccattc gcgaactggc gtttttgccg gggacggccc ggctggcggt
     2341 ggtccgctcg aacggggaac ttgttctgct ggacgccgag ggggatggta cggccgggcc
     2401 ggcttcggct gggaccagcc cggtcccgcc gcctgccgcg tggatgattt tggaatcgcc
     2461 cgctttcgcc atgtcgtttg atgggcgcgg tgagcagttg gcgctcgctt gcgatgacgg
     2521 ctttatccgg atcgtcgcgc tggcggatgc caagctgaag ggccgcatcc gcccgcccga
     2581 accgatacgc agcctggcct tcaacccggg cgggcgcgtg cttctgacgc tggatgatca
     2641 gggcaaggtc agcatcctcg atctggcgac gggcgacagc gtcctgcacg tgccgctgac
     2701 cacgggccag gccggcctgc tggccacgtg gattggcggc aacgacgccg tgctgttggg
     2761 ccagccggga ggtttatacg aacacgactt cgcgcgggtc gatgcgctga tcgcccaaaa
     2821 cgtctcttat gcgcggcaac gcgcggtggc gcggcaattg gccgaggggg atgtcgagcg
     2881 cgcgtgggaa cggagcgacg cgctggaact ggtagacccg tggcgcgccg ctggcatgcg
     2941 cctgctcgtg ctcgaaaccg cattgcgccg ccgctcgcaa gtaatcccgt cggcctggct
     3001 cgcggttacc gcgtcggcta cggaagccgc cgtttggctg cggctgggac acgcggcata
     3061 cgagggcgag cgcttcgcgg aggccaagac ctggctggaa cgcagcgccg aactgagcgg
     3121 cactcaactc gacgccagca gcgaacggcg catcgcccaa tgccggtatc tggccggcga
     3181 gtacctggcg gcggccgaag ccttcgccca gctttcagct cggccggatc tcgacagcac
     3241 gcttctgccg gccgtacagc ttgagcaagt tgccgccttg gttctcgggc aggagacggc
     3301 caaagcacgc tctgtggccg cccgcatcgg cggcctgggg cgtgcgggtg tgcgcctgga
     3361 tcctggcgcg acgggtgcag cgcggcagat tgcacgcacg ctaacggggc tggagtctga
     3421 aattcccaca atgccgatcg gtctcgaagc tgatggtccg gtcaggctgc tcacgcaact
     3481 cgccgaagca aacctgcgca gcatggattt gcgctttcac gatgatgccc aattcttcgc
     3541 gggcgagctg gcacgcgctt ccgggcagac gcagcgcgcc atcgcggcct atcagcgctg
     3601 catcgacctg gcgcgtgacg agtggccttc gggctgggcc cgctatcgcc tgcgccaatt
     3661 gtcggatggc atttgatcgg ctaccgtgcc cgccatgaac gttctggtta tcggctcggg
     3721 cggccgtgag cacgccctct gctatcgtct ggcgcgttcg ccgcgcttgg acaaactgtt
     3781 cattctgccg ggcaacgccg gcacggccgc gctgggtaca aacattcccg gcgacgccgc
     3841 cgacatccgg cttgctctgg aggtggcgcg gcgcgagtca atcgatttga cgatcgtcgg
     3901 tccggaagac cccctggcgg ccggtatcgt ggatgccttc gaggaagccg gcctgcggat
     3961 tttcggtccg accgctgccg cggcgcgcct ggaatccgac aagtcattcg ccaagactct
     4021 aatgcgtcaa caggcggtgc cgacggccga cgcacgcatc ttcgacaatt acgaccaagc
     4081 gcggcaatac atcgcgaccc gcgacgagcc ggtggtgatc aaggccgctg gcttggccaa
     4141 aggcaagggc gtgatcgtct gcgaggaccc ggcggacggg attctggccg ccgagcggat
     4201 gatggtgaac cgcattttcg gcgacgccgg cgcgcggatc atagtggaag aacgcctgaa
     4261 cggccgggaa gcgtcactgc tggccattgt ggatggacaa acgatcttcg tgctggagcc
     4321 ggcccaggac tacaaacgcc tgggggcggg cgataccggg ccgaacaccg gcgggatggg
     4381 ctcgttttcg ccgacatcgg ttctcgacga cgagcttctg gcggagattc agcgtcaaat
     4441 tctcgtaccc acgctggatg gattgacgcg cgaaggtatt cactattgtg gcgtcctcta
     4501 cgccggactg atgctcacgc ccgccggtcc aaaggtcctc gaattcaact gccgcttcgg
     4561 cgacccggag acccaggcga tactcatgcg gctgcaatcc gacttgcttg aactgctcga
     4621 cgcagccgta tcagggcacc tggaggatgc cgcggtgcgt tgggatccgc ggcatgcact
     4681 gtgcgtggta atggcgtcgc agggctatcc tgacaagtac cgcaccgggc tgccgataac
     4741 gggtctgccc gaagacgccc tgcgcgatga cttggcgatc tttcacgccg gcacgcgcat
     4801 acaggacgga caggtagtga cgtccggcgg gcgggtgttg ggcgtgactg cgttgggcgg
     4861 cagtttgagc gacgcgcggc gggtcgccta cgaagcggtc ggtcgaatcc acttcgaagg
     4921 cgcgtatttc agacccgact tagggcagga gtgagcgagg tcgatcgcgt tactgccacg
     4981 cctcgatcgt ccgcgcctgc ttcggcgatt tcctcgatac atccagtagc agggtcgtgt
     5041 tgcgccacga accctcgaag cgcccgaccg actcgccgcc gggtatggtc acgctgattt
     5101 tgctgaccgt gccgctgcca agcccgaagt gtaactggtt ggacttgatg gccaatccgc
     5161 cgcaactcac atccaggaat gcgcggcgct tctcgccgtt gttgagcgta atctcgacta
     5221 cggccccatt tagagcgcgg tcggcagcgc tgagaacgag attcaagtag ccggcgtttt
     5281 ccgcacccga tttgcccaag ccccgtccgc gcaggagcgt cgtccgcgtc ttggtcgggt
     5341 cgaaccaatc gatcttgtct gacttcttgc cggtattgga accgacgagc agatcacacc
     5401 agccgtcatt atcgaaatcg cccgcggcga cgcccgcgct gtcctctatc cgcaacttgt
     5461 tgcgctcctt gtcaacgcga acgaaagact gcccgccctg gcccagccac agcttgctgt
     5521 acccggaatc cagcttgcca tatttcagtg gcgcatgggg atggttcttt ccgtcatcgg
     5581 cggattcagt cacgaacaga tcgagccagc cgtcgttgtt cagatcagcc cagaccgccg
     5641 actgccacgt cgcctccgcc ttgtagccct ccggcggctc ccaggccagt gcgcactccg
     5701 cagttacatc ggtgaaacga aggccgccgt cgggacttga gtcgttgcgc aacagcatcg
     5761 accgcggctg tgacttcttg gggtcgtcgt gcgcgtaatt gaccacgaat acgtcgaagt
     5821 cgccatcatt gtcatagtct gcaaccgcca cggcgcgcga actgcctgca ccacccgttg
     5881 cgcccgcctc ctcagcgacg tccttgaaag tgccgtcgcc ctggttgtgc cacagccaat
     5941 tcggcgagcg ggagttggct acatagatgt cgacgaagcc gtcattgtcg aaatcgcaga
     6001 acgccacagc ggtggcggcc cgcggttcga cttgcaatcc cgccgcttcg gtgacgttcg
     6061 taaaatgtcc cgtgccgtcg cccttgaaaa gccggcaggg aacggcccgg gtctgcttgg
     6121 gtgtatccgt gatttcaaac cccgccacga acaggtcgag gttgccgtcg ttgtcgtaat
     6181 ccaggaagcc gtgcgcgcgg ctcacgtcca gaagtttttc agtcggctga ttaatggcgg
     6241 catcgcgttg cgggccggac tgcgcttcgg gggcgtccgt caagccggac ttactcagca
     6301 cgtcggtaaa cgtgccgtcg ccgtcgttgc gcagcagcca gtgttcggct ttgactttct
     6361 cgaccgttgg atccggttcc tctttttgtg gttctgccgg cccgcgtttt gtgcgggact
     6421 gtcgagcacg ctccgctttc tctcgtcctg ttttggtgtc ttctgggtcc agtttaacgg
     6481 aaatcttgcg ccactccgtt gagaaggcgt cgaggtcgcc gtcattgtcg aagtcgccga
     6541 agctgataaa cgtgccgcgc agaaccagcg gtgcttcgaa gcggttgcgg ccgttgttca
     6601 cgccgacgcg attgcgcgtt acaacatcga cgtagccgtc cagatcgatg tcgaccagtc
     6661 cgactatggc gtcgtcgggc atgggatatg gcaagccgac ttcggcgctc acggtgacga
     6721 aggtcggtag agcgggttcg gccgccgctt gcgccagcgc gccggcggcg cagttgcaga
     6781 tgatcagcgt ggcgagagtt tcacagaagc gaagagacat tcgtcactcc agtcttggcg
     6841 tgtccgttct ttcagagcag ctttatacca acacgggcgc gcgagtggaa cgttgcggcg
     6901 tgcacgttcc gcccccttga aatccgcccg ataacaacgc gagggcggac gccggcgtgt
     6961 acgaagaacc cacggccaag acctcggtga acaccggggc gcgaaaaccg gctctggttc
     7021 taggctggcg gcatcgatgg ccgcgttggt gcttccggag gaagagtgat gtctgtagct
     7081 cgaatttggt tggctctggc cgctctggcc ttggcgggcg gtcaatctcc gactgatggc
     7141 ccgcaaacca gtccgggcgg gccgatcatt cccgacctgc cgcacaagga cttcagcggc
     7201 gccacagctt gcccggtagt gcgcgtctgc cgcggcgata ccgtcatcgt ccgtatgggc
     7261 gaagaagcca aacgcctcgg cctgataggc gtccaagtgc cgggcctggc ggacccgacc
     7321 ggccgggcgg cggaaagatt cctcgagaac ctgctggccg gcgaggccgt gtatgtggag
     7381 tttgcagaag ccagtccggc cccggaccac tttggctgca tcccggcgta ccttttccgt
     7441 gctccggacg gcctgttcgt gaacctggaa ttggtccggc aaggatacgc tgccgttccc
     7501 gaggcgggcg cgttcgagta tcgggcggtc ttcgagcgtc ttcagcagcg cgcccgggca
     7561 gccggcaagg gccgctgggg cgaacgtacg acggattcac aagccgaagc aaagccgggt
     7621 gaacgaagcc ctggcccgga ccgcgttcca aagagcgagg cgcgcgacgg gggtcaatct
     7681 actccggcaa gccggtccag cccatccccg gtgccggggc ctgccgcaca atccggggcc
     7741 ggcggtgatg cagcggacga cgcggtcgtt tacatcaccg ccaaaggcgc caaatatcac
     7801 cgtgaaggct gccgccacgt tcgcaagagt ggccggccgg tcagcctggg cgaggctcgc
     7861 acgcggggtt tgacgccgtg ttcgcagtgc aagccgccgg agtgacgcct gccgcgggcg
     7921 tggttgtggt ttgcgcggat tgggcgaggt ggcgctgttt cggggcgggt ttgcggggat
     7981 tgggcgaagg tggcggtatt ccggggcggc acgccccggc tctgctacgc tcgcccgcgg
     8041 ccgcgttgcg aatcctgcgc gtacagggta ccttcaggta acgcttgctg cgcggcaggc
     8101 tcttgcagcg catagcggct ggctttcgca gcgcggtggc ggcaggctct cgcagcgcat
     8161 agcgcccgta gctcaactgg atagagcatc ggacttcgga tccgacggtt ggcggttcga
     8221 atccgtccgg gcgcgttacc ggtcctgctt ttctggagga agaacgatgc gtcgaccgct
     8281 cgtagccggc aattggaaaa tgaacctcac cttgaccgag tcgcgcgacc tcacaagaaa
     8341 cgtcgccgcc cgcctttcgc cggaggccgc cgcgaaggtc gaggttgccg tctgcccgcc
     8401 cttcgtctac ctgctccccg tgctcgaggc cgccaaggag acgcccgtcc aagtcggcgc
     8461 ccagaacctc tattttgagc cgaagggcgc cttcaccggc gaagtctcgg ccgccatgat
     8521 cgctgaattg ggcgccaagt acgtcatcgt cggccacagc gagcgccgcc ataccatcgg
     8581 gcaccacgaa gacgatcgca tgatcaatct caaactgcgc gccgcgcggg gcctcggcct
     8641 cacgccgatt ctctgcgtcg gcgaaaccct gctggagcgc gaggccgagc agacc
//
LOCUS       DAOSYS010000155         8662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6936_length_8662_cov_5.884396, whole genome shotgun sequence.
ACCESSION   DAOSYS010000155 DAOSYS010000000
VERSION     DAOSYS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_8662_cov_5.884396"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PLU99_10240"
     CDS             <1..499
                     /locus_tag="PLU99_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17476.1"
                     /translation="GVVAAPTLEVIARNTLGYVPESAAGTSPPAGAPQRVAVILDARG
                     GRIYAALFELRESQANEITVLQPAGAFEPAAWFAQLGAPVWVMGEGLRKHGAAVAATG
                     AYMLPEALWAPRAENVAVIGSRLAEAGQFCRPDQIVPHYIRPPECEEVYEQRRAEARR
                     RRNGV"
     gene            complement(681..1544)
                     /locus_tag="PLU99_10245"
     CDS             complement(681..1544)
                     /locus_tag="PLU99_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO17477.1"
                     /translation="MTRIFTHLPALFAVAVLAAFGARAEILFDNGPFITNPTGGTGPI
                     AGLPISQAEGFTMPGSSFVYSTTGIGATYADNTAVAEDFFVPAGGWDLDSVTLFAFQT
                     SQTTPTIETVRINIWNATPYSAGSPPPVPDPLPQPLMAEPLVLDAGPGAFVCHRQSPS
                     GTSTVRPVYAYTVSLDGLPDGGRLEAGHYWLEWSFIGAATPTPRCWIPLVTPRASAFN
                     QNARLFNSIDGSAAGPRVWFEGREGYSPPVSEGWPYALPFVLNGTVPEPSALLALLSA
                     TALALARRRSD"
     gene            complement(1658..2317)
                     /locus_tag="PLU99_10250"
     CDS             complement(1658..2317)
                     /locus_tag="PLU99_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOO17478.1"
                     /translation="MSVHTETEAKFKLDQPQDWPARLRAAGGVRLARVFEINRLFDTS
                     DDRLKKADCGLRLREWHTLEAGDDPAAASEPAAQSGTTAPSRNGALLTFKGPRAAGGL
                     KSRAEIETALDDAAAGGQILTRLGVREVLVFEKVRETWRLGPCEVALDHLPRLGWYLE
                     IEGHDADAVSQVRRQLGLADAPLVGETYPALAAMAGDRDAAGRVSLLFPPGERPQVPN
                     A"
     gene            complement(2521..3894)
                     /locus_tag="PLU99_10255"
     CDS             complement(2521..3894)
                     /locus_tag="PLU99_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO17479.1"
                     /translation="MSIRVLVVEDESLIRWSLRQKFEERSYRVTEAETGKTALEALDT
                     GIYDLVLLDYKLPDVTGLDVLRKLREMDTDSVVVMMTAYSTIENAVEAIKLGAYDYIA
                     KPFQMDQLMLTVDKALATTQLRREVRELRRHLQHEFGIDSIIGQHPSMLKLFDMIRQV
                     ARAGVSTVFLRGDTGTGKDLVARVIHHTSDRATAPFVNITCTALSESLLESELFGHER
                     GAFTDAKQQKKGLFELADGGTVFLDEVGDMPPALQAKLLRFLEERRFRRVGGTKEISV
                     DVRVIGATNRDLEKAIEDGQFRRDLMYRLNVVTIYIPPLRARGDDIKLLTQHFVARFA
                     KEFKKPLTKVSDEAYQMLYNYSWPGNVRELRNVIERAVLLSKTDTVTPEDIVLGRAES
                     HSWEWDIENVTLPPEGFDFEKLRKLERHLLKQALARTNNNQTQAAKLLNLSRDRLRYR
                     LQKHGLL"
     gene            complement(3891..5096)
                     /locus_tag="PLU99_10260"
     CDS             complement(3891..5096)
                     /locus_tag="PLU99_10260"
                     /inference="COORDINATES: protein motif:HMM:NF022995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HOO17480.1"
                     /translation="MPSFSLKELEHYIGFDEQDAANLHALAPYVRPMLPGTIDRFYDE
                     IQKHAGARVLMSGGTPQAVRLRGTLARWLEQLFRGTYDDDYWKSRERIGEVHVRIGLP
                     QQYMFAAMQVIWAELERGIRGLGLPNISAKLSSLHKLLTLELAVMLDSYKEHYSQQIR
                     GVERDAVQERLTEAEHLAQIGHLAASLAHEIKNPLAGISGAIQVIRDSMKSSDPHRPI
                     LDEILRQIGRLDNTVKDLLIYARPKPPRFRKCAIQDVIAGALPVWRQQPEIQRVNFEY
                     VNSRALPVIAADENQIEQLLTNLLLNAAQASKPGGLVRLTAAPQPDGIRLSIEDRGHG
                     MDQQVCKRAAEPFFTTKARGTGLGLSICKKIVETHGGALTIKSVVGEGTEVIVDLPRR
                     QPAVESEAG"
     gene            5470..6693
                     /locus_tag="PLU99_10265"
     CDS             5470..6693
                     /locus_tag="PLU99_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOO17481.1"
                     /translation="MKHSLVVTGLLALGLLGGCPNNVQPDPAVKAKITASATSIEPGD
                     TLVFDASQSTSKNGGALTYAWDFAGEGTATTATATHIFAAPGLYTVTLTVADETGATG
                     VSTLDICVNGAAPTAVIAADVTSGPAALVVQFDGSGSSAPDDDIRDYIWSFGDGGSAT
                     VPNPLHVFTDPGTYTVRLTVRTFGCVEGEATATITVEASLTHSLQFGGSEVALLSLGG
                     SQTLTDFTFEAWAKPADAGGTVVNTLDNRLVLEMLPGANKLRYTVGEASFEASAVGLA
                     NSWHHVALGRQASGRTVLYLDGNQISSSPSAGQLVAEQLTLGGLGAFSGKIAEVRLWT
                     ELRSQLQISLNMNRRLPAPIAEANLLGYWRIDENGSSQMLQNLGRSAIPGMLGTSGAV
                     DARDPAWSTDGPELD"
     gene            complement(6698..7729)
                     /locus_tag="PLU99_10270"
     CDS             complement(6698..7729)
                     /locus_tag="PLU99_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOO17482.1"
                     /translation="MKASSLRADVVLLIAAVLWGAAFVAQRAAMRHMGPLTFNGLRFA
                     LGALALAAVIGLRRTRRRPAPDALKEPSSYEPPRDAAFRLYLLGGVVAGLVLFAGASL
                     QQAGLVYTTAGKAGFITGLYLPLVPILGLVLGRRTGGATWLGVVLAVIGLYLLSIVPG
                     SFEMERGDALVAACAVIWAVHVLVVAHLAPRLEPLKFGLVQFSTVAVASVAAAAAFEP
                     LAGANLRGGALALLYGGLVSVGIAYTLQLVGQREAPPGHAALILSLEAVFAALTGGLL
                     LDERLAQRQLAGAALMLCGMLVSQLPRLRRAPERPGSFDPVATTEHSPASSAGGEVKN
                     ELLKSRRND"
     gene            complement(7840..8340)
                     /locus_tag="PLU99_10275"
     CDS             complement(7840..8340)
                     /locus_tag="PLU99_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17483.1"
                     /translation="MALNRSLCRGVLVCALLLPTVATAQWYDDSWGGVYWDDDYYYAA
                     ADPARAYTVDYGGGRWSYGPYSFEFRRSPYGNWYYGAATPYAYSYGYRTPYWYAYGTP
                     YWYGYTSPYAYAGAYEYAPGYYAGPPATFYGPPMYYYGPPTYYYYYDDDDLDDKIEAW
                     LADLFD"
BASE COUNT         1487 a         2699 c         2933 g         1543 t
ORIGIN      
        1 cggcgtggtc gcggcgccga cgctggaagt gatcgcccgt aacaccctcg gctacgtgcc
       61 cgaatccgcg gccggcacgt ctccgccggc cggggctccg cagcgcgtgg cagtcatcct
      121 tgacgcgcgc ggcgggcgaa tctacgcggc attgttcgag ctgcgcgagt cacaggcgaa
      181 cgagatcacg gtgctccaac cggccggcgc gttcgagccc gcggcctggt tcgcgcaact
      241 cggagcgccc gtttgggtca tgggcgaggg tctgcgaaag cacggagcgg cagtggcggc
      301 gaccggcgcc tacatgctcc cagaggcgct ctgggcgccg cgcgctgaaa acgtggcggt
      361 catcggcagc cggcttgcag aagccgggca attctgccgt ccggaccaga tcgtgccgca
      421 ctacatccgc ccgcccgaat gcgaagaagt ctacgaacag cgccgcgccg aagcccggcg
      481 gcgccgcaac ggagtgtgaa acggggacgc ggcgtcccgt ttcggaacgt cgttccggac
      541 gtgcccgtta aacccacagc agatgcgtcc ccctttttga acccccgaac ctgcgtcccc
      601 gtttcgcgcc cccgtttcga acgccatttc gaacgtgcga aacgttaggc gccatgtcgc
      661 ggtgcgagtg ccaggaaacg tcagtcgctg cgccgccttg ccagcgccag ggccgttgcg
      721 ctcagcaaag caagcagtgc agacggctcc ggaacagtgc cgttcagaac gaacggcaga
      781 gcatacggcc agccttcgga cacgggcggc gagtatccct cgcggccctc gaaccacaca
      841 cgcgggccgg cggcgctgcc gtcgatcgaa ttgaacaggc gtgcattctg gttgaaggcg
      901 gacgcgcgcg gcgtaacgag tggaatccag cagcgcggcg tcggggtagc ggcgccaatg
      961 aaggaccatt cgagccagta atggccagcc tcgagccggc cgccgtcagg caatccgtcc
     1021 agcgagacgg tgtaggcgta gaccgggcgg acggtgctgg tgccgctggg gctttgacgg
     1081 tgacagacga acgcgcccgg gccggcgtcg aggacgaggg gttcggccat gagcggctga
     1141 ggcagggggt cgggtacggg cggtggcgag ccggcggaat atggagttgc gttccagatg
     1201 ttgatgcgga cggtctcgat ggtcggagtg gtctgcgagg tctggaaagc gaacagggtc
     1261 accgagtcga ggtcccagcc gccggcgggc acgaagaagt cctccgcgac ggcggtgttg
     1321 tcggcgtaag tggcgccgat gccggtcgtg gagtacacga aggagctgcc gggcatggtg
     1381 aagccttcgg cctgactgat aggcaggccg gcgatagggc cggtgccgcc ggtgggattg
     1441 gtgatgaagg ggccgttgtc gaagaggatt tcggcgcggg cgccgaaagc ggcgaggacg
     1501 gcgacggcga acaaggcggg cagatgtgtg aagatgcggg tcattttttc tcctcctcgg
     1561 cggtaatgat agccggaggc cgggcggggg gaaggcgacg agggattgag agtggtagga
     1621 ccacagatgc gtccccgttt cgggacgccg gcagagctca ggcattcgga acctgcggcc
     1681 gttcgcccgg cgggaaaagc aggctgacgc ggccggcggc gtcgcgatcg ccagccatgg
     1741 cggccagcgc cgggtaggtt tcgccgacca gcggcgcgtc ggccaggcct agttggcggc
     1801 gcacctgact gaccgcgtcg gcgtcgtggc cctcgatttc caggtaccag cccaggcgcg
     1861 gcaagtggtc cagggcgact tcacacggtc ccagtcgcca ggtttcgcga actttctcaa
     1921 aaaccagcac ttcgcgtacg ccgagacggg tcagaatctg cccgccggcc gcggcgtcgt
     1981 ccagcgcggt ctcgatttcg gcgcgcgact taaggccccc ggccgcgcgt ggtcctttga
     2041 aggtgagcag cgcgccgttc ctgctcgggg cggttgttcc gctctgcgcg gccggttcgc
     2101 tcgcggcggc cgggtcgtcg cccgcctcga gggtgtgcca ctcgcgcagc cgcaagccgc
     2161 agtcggcttt tttcagccgg tcgtcggaag tgtcgaacag gcggtttatc tcgaacacgc
     2221 gtgcgaggcg tacgccgccg gcggcgcgga ggcgtgccgg ccagtcctga ggctggtcga
     2281 gtttgaactt ggcttcggtt tcggtgtgca ctgacatatc tcagcccgcc attcttttgt
     2341 cgggaatagg atgctacccg gctggagcgc agtttcccgc ctgcggccgt tttagcgaat
     2401 ccggctgacg atgttacaag tgaagaccat ttgtggccac acggcggagt tttccgacac
     2461 tgttgaggca caggcctcaa cagtggcaca ccggtagcac acggcgcggt ttcccgctgg
     2521 ttagagcaag ccgtgctttt gtaagcggta gcgcaggcgg tcgcgcgaga ggttcagcag
     2581 cttggcggcc tgggtctggt tgttgttggt ccgcgccagg gcctgcttga gcaggtgccg
     2641 ctcgagcttg cggagctttt caaaatcgaa accctccggc ggcaacgtca cgttctcgat
     2701 gtcccattcc caggagtgcg actcggcccg gccaaggacg atgtcttcgg gcgtgaccgt
     2761 gtcggtcttg ctgagcagca cggcccgttc gatcacgttg cgcagctcgc gcacgttgcc
     2821 cggccaactg tagttgtaaa gcatctggta ggcttcgtcg ctcaccttgg taagcggctt
     2881 tttgaactcc ttggcgaagc gggccacgaa atgctgcgtc agcagcttga tgtcgtcgcc
     2941 ccgcgcccgc agcggcggaa tgtaaatggt gaccacgttg agccggtaca tcaggtcgcg
     3001 ccggaactgg ccgtcctcga tggccttttc gaggtcgcgg ttggtggccc caatcacgcg
     3061 cacatcgacg ctgatttcct tcgtgccgcc gacgcgccgg aagcggcgtt cctccagaaa
     3121 gcgcagcagc ttggcctgca gggccggggg catgtcgccg acttcgtcca gaaacaccgt
     3181 gccgccgtcg gccaattcga acaagccttt cttctgctgc ttggcgtcgg tgaaggcgcc
     3241 gcgctcgtga ccgaaaagct cgctttccag cagcgactcg gaaagggcgg tgcaggtgat
     3301 gttcacgaag ggcgcggtgg cccggtcgga ggtgtggtga atgacgcgcg ccaccaggtc
     3361 cttgccggtg ccggtatcgc cgcggaggaa gaccgtggac acgccggccc gcgccacctg
     3421 ccggatcatg tcgaacagct tcagcatgga ggggtgctgg ccgatgatgg agtcgatccc
     3481 gaactcgtgt tgcaggtgtc gccgcagttc gcgcacctcg cggcggagct gcgtggtggc
     3541 caaggcctta tccacggtca gcatgagctg atccatctgg aaaggcttgg cgatgtagtc
     3601 gtaggccccg agcttgatcg cctcgaccgc gttctcgatg gtgctgtagg cggtcatcat
     3661 cacgacgacc gaatccgtgt ccatctcgcg gagcttgcgc agcacgtcca gcccggtcac
     3721 gtccggcagc ttgtaatcca gcaggaccag gtcgtagatg ccggtatcga gggcttccag
     3781 ggctgtcttg ccggtctcgg cctcggtcac gcggtagctg cgctcctcga atttctggcg
     3841 caacgaccaa cgaatcaggc tctcgtcttc aacgaccagc acacggatac tcatcctgcc
     3901 tcgctttcca ccgccggctg ccgccgcggg aggtccacaa ttacctcggt gccctcaccg
     3961 accacgctct tgatcgtcag ggcgccccca tgagtctcga cgattttctt gcagattgac
     4021 agtcccagcc ccgtgccgcg ggccttggtc gtgaaaaacg gctccgccgc tcgcttgcaa
     4081 acctgctggt ccatgccgtg tccgcgatcc tcgatcgaga ggcggatgcc gtccggctgc
     4141 ggagccgcgg tcaggcgtac cagcccgccc ggcttggacg cctgggccgc attcagcagg
     4201 aggttcgtca aaagttgttc tatctgattc tcgtccgccg cgatgaccgg caaggcccgg
     4261 ctgtttacgt actcgaagtt gacgcgctgg atttccggct gctgccgcca gacgggcagc
     4321 gccccggcga tgacgtcctg aatggcgcat ttgcggaagc gcggcggctt ggggcgcgcg
     4381 tagatcagca ggtccttgac ggtgttgtcc agccggccga tctgccgcag aatttcgtcg
     4441 agaatcgggc ggtgcgggtc gctcgacttc attgagtcgc ggatgacctg gatcgcgccg
     4501 ctgatgccgg ccagcgggtt tttgatctcg tgcgccaggg aggcggccag gtgcccgatc
     4561 tgggcaaggt gttcggcctc ggtcaggcgc tcctggacgg cgtcgcgctc aacgccgcgg
     4621 atctgctgcg agtagtgctc cttgtagctg tcgagcatga cggccagctc aagcgtcagc
     4681 agcttgtgga gcgagctgag cttggccgag atgttgggca ggcccaggcc gcggattccg
     4741 cgttccaact cggcccagat gacctgcatg gcggcgaaca tgtactgctg cggcaggccg
     4801 atgcggacgt ggacctcgcc gatccgctcg cggcttttcc agtagtcgtc gtcgtaggtg
     4861 ccgcgaaaga gctgttcgag ccatctggcg agtgtgccgc gcaggcggac ggcctgcggc
     4921 gttccgccgc tcatgagcac gcgtgctccg gcgtgtttct ggatttcgtc gtagaagcgg
     4981 tcgatggtcc ccgggagcat cgggcgcaca tacggagcca gggcgtggag gtttgccgcg
     5041 tcctgctcgt caaacccgat gtagtgttcc aactccttga gcgagaagct cggcatagac
     5101 cttacattat attctatatt gaccgctcgc gtagccctcg gtttgctgct gttcgccgct
     5161 cgcgtagccc tcggtttact gtgctagcgc cgcatcgcgt cttagaggac cgacgagcgc
     5221 gttggtgcca ccgctcgggc ttcggggccg catgcagcac tgcttgaagc cgcgcttcat
     5281 gcagtgccac acacaggcgc gttcgcacgg cgggtgggcc actcagcggc cggttgcctg
     5341 ggacacgccg gcttgcccta taatgccgcg ttcgctgatt gcccggctgg cgcactgttc
     5401 cggatgtgca gatagccgcc aggccgcggg cttcggagct cccgacaagg ctccgcgtgg
     5461 agatggcaga tgaagcactc gctcgtcgta accggtttgc tggctttggg gcttttgggc
     5521 ggctgcccga ataacgtgca acccgacccg gccgtgaagg ccaagattac ggccagcgcc
     5581 acgtcaatcg agccgggcga cacgctcgtg ttcgacgcca gccagtcaac ctccaagaac
     5641 ggcggcgccc tgacttatgc ctgggatttc gcgggcgagg ggactgctac caccgctacc
     5701 gccacgcaca tattcgccgc gccgggcctg tatacggtga ccttgacggt cgccgacgaa
     5761 accggcgcca cgggcgtctc caccctggac atttgcgtga acggcgccgc gcccacggcc
     5821 gtcatcgcgg ccgacgtcac gtcgggaccg gccgcattgg tggtccagtt cgatggctcc
     5881 ggcagcagcg cgccggacga cgatattcgc gattacatct ggagcttcgg cgacggcggt
     5941 tcggccaccg tcccgaaccc gctgcacgta ttcacggacc ccggcacgta cacggtgcgg
     6001 ctgacggtgc gcacgttcgg ctgcgttgag ggcgaggcga cggccacgat cacggttgaa
     6061 gcctcgttga cccactcgct gcagttcggc ggatctgaag tcgcgctgct gtcgctgggc
     6121 ggctcacaga ctcttaccga tttcacgttc gaagcctggg ccaagccggc cgacgcgggt
     6181 gggacggtcg taaacacgct cgacaaccgg ctggtgctcg agatgcttcc gggcgccaac
     6241 aagctgcgct acaccgtggg agaggccagc ttcgaagcgt ccgcggttgg actggcgaat
     6301 tcctggcacc acgtcgcgct tggcaggcag gccagcggcc gcacggtgct ttatcttgac
     6361 ggcaaccaga taagcagcag cccttcggcg gggcaattag tcgccgagca actgacattg
     6421 ggcggattgg gggcgttttc cgggaagatc gctgaagtgc gcctgtggac tgagttgcgg
     6481 tcgcagttgc agatctcact gaacatgaat cgacggttgc cggcgccgat cgccgaggcc
     6541 aatcttttgg gatactggag aatcgacgag aacggctcgt cgcaaatgct ccagaatctc
     6601 ggcaggtccg caatcccggg gatgctgggt acgtccggag ccgtggatgc cagagatccg
     6661 gcgtggagca cggacgggcc ggaattggac taagatttca gtcgttccta cgggatttga
     6721 ggagttcgtt tttaacttct cccccagcac tgcttgctgg gctgtgttca gtcgtcgcta
     6781 cgggatcaaa ggaacccggc cgttccgggg cgcgtcgcag gcgcggcagt tgtgacacga
     6841 gcattccgca cagcatgagt gcggcccccg ccaactggcg ctgcgccagg cgttcgtcca
     6901 ggagcaagcc gcccgtcaga gccgcaaaaa ccgcctccag gctcaggatc agggccgcgt
     6961 gcccgggcgg ggcctcgcgc tgcccgacga gctgcagtgt gtaggcgatg ccgaccgata
     7021 ccagcccgcc gtagaggagg gccagggcgc cgccgcggag gttggcgccg gccagcggtt
     7081 caaatgccgc ggcggcggcc acgctggcga cggcgaccgt ggagaactgc accaacccga
     7141 acttgagcgg ctccaggcgc ggcgccaaat gggccacaac cagcacatgt accgcccaaa
     7201 tcacggcgca ggccgcgacc agcgcgtctc ctcgctccat ctcaaatgac ccggggacga
     7261 tactgagcag gtagagaccg atgacggcca gcaccacgcc cagccaggtc gctccccccg
     7321 tccgacgccc aagcacgagc cccagaatcg gcaccagcgg aagatacaag cccgtgatga
     7381 aaccggcttt tccggcggtt gtgtaaacca ggcccgcttg ttgcagcgaa gccccggcga
     7441 aaagcaccag cccggcgacg acgccgccca acaggtagag gcggaatgca gcatcgcggg
     7501 gtggttcgta gctgcttggt tctttgagcg cgtcaggcgc gggccgccgg cgcgtccgcc
     7561 gcaagccgat gacagccgcc agggcgagcg cgcccagcgc aaaacgcaat ccgttgaacg
     7621 tcagcgggcc catgtgacgc atcgccgcgc gctgcgccac gaatgccgcc ccccacagca
     7681 cggccgcaat cagcagcacc acgtcagccc gcagtgaact cgctttcatt cgggcagagt
     7741 ataacgctcg cgcggcaagt cgcggatagg cccggaacgc ccccccgttc cggcgctcgg
     7801 ataaaccggc cgcgggcgct ggtgccggcc ggtgcgggtt cagtcaaaca agtctgctag
     7861 ccaggcctcg attttgtcat cgagatcgtc gtcgtcatag tagtagtaat acgtgggtgg
     7921 tccgtagtag tacatgggcg gtccatagaa cgtagccgga ggcccggcat agtagccggg
     7981 cgcatactcg taagcgccgg cgtaagcata gggcgaggtg tatccatacc agtatggcgt
     8041 tccgtaggcg taccagtatg gcgttctata gccgtaggaa taggcatacg gcgtggcggc
     8101 gccgtagtac cagttgccgt aggggctgcg gcggaactcg aatgaatacg ggccgtagct
     8161 ccaacgtccg ccgccgtagt cgaccgtgta tgcacgcgcc gggtcggcag cggcataata
     8221 gtagtcatcg tcccaataga ctccgcccca actgtcgtcg taccactggg ccgtggcgac
     8281 agtgggcagc agcaatgcgc agacgagtac ccctctgcat agtgagcgat tcagcgccat
     8341 gtgcggctcc ttaaagatga cgagctaccc tgtcggacaa cgttcgcttt acagcagtgt
     8401 gccgttgaaa gctgaaagcc gcctgaaagg ggcgttaaag acgtgaaggc gctggctttc
     8461 agaggtcgaa acaaatgacc ggtatgtgtg agctgaaatc cggtctacga ccggactgat
     8521 gaaccatttg ctactcgggg tggcgttaat atcgtgtgtg gtcggcggcg tggggcgcac
     8581 gcgcgaaatt aataagcttg atccgataga ggcgctcggc tacgaatagc acctgtcagc
     8641 gcggcggaat cccacagagc gc
//
LOCUS       DAOSYS010000156         8660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_6939_length_8660_cov_5.608716, whole genome shotgun sequence.
ACCESSION   DAOSYS010000156 DAOSYS010000000
VERSION     DAOSYS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6939_length_8660_cov_5.608716"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..316
                     /locus_tag="PLU99_10280"
     tRNA            245..316
                     /locus_tag="PLU99_10280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:278..280,aa:Gln,seq:ttg)
     gene            371..1372
                     /locus_tag="PLU99_10285"
     CDS             371..1372
                     /locus_tag="PLU99_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOO17484.1"
                     /translation="MTVGTGEKTTPTAPRVGAVILAAGKGTRLKTELPKVLHEVCGRP
                     MLAYVIDACRAAGVKECVVVVGYQKDLVIRAFTEDRDLTWVEQNPQLGTGHAVMVCRE
                     HLAGRFDHLFVLAGDGPLIRAETLRTLIERHLAEQAAVTLATAVLDDPTGYGRIWRDA
                     EGRLLGIVEHGDATPEQKRIHEVNPSYYCFKQPELLAALDQVRPNNVKNEYYITDAIS
                     ILLAGGHKVQAITSVPPQDIFSINSRQELAMVNAVMRDRILDRLMSSGVTIVDPRTTW
                     IDDRATIGQDTVIHPNVVISGPAIIGRGCRIGPFVNLTGAARVPDHGVISPFAGGVA"
     gene            1369..2313
                     /locus_tag="PLU99_10290"
     CDS             1369..2313
                     /locus_tag="PLU99_10290"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOO17485.1"
                     /translation="MSDMRIFSGRSNPAMAQSICQYLGLPLGRAQLDNFPDGEIQLKL
                     FEDVRGRDTYVIQSTCSPVNDNLLELLIFIDCLKRASAERITAVIPYFGYARQDRKDE
                     GRVPITAKLVANILTEAGADRVLTVELHAAQIQGFFDIPVDNMSAQPVFQAYFETLDL
                     QPLTLVSPDVGNAKHARVYADRMGANLAIIDKRRISGSEAAPLALIGNVADQNVLMID
                     DIIATAGTVVQAAKLVRQHGARRIVVAATHGVLCGPAIERLAGAPIDHIAVTDTIPIP
                     EQVRARLPNLSVLTVSELIGEAVRRIHRHESVSSLFMK"
     gene            2392..3048
                     /locus_tag="PLU99_10295"
     CDS             2392..3048
                     /locus_tag="PLU99_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOO17486.1"
                     /translation="MEIAKIRGEARQPGNRHANERLRRRGLIPAVIYGHGQAPETVAV
                     SQHDLELVLGRAHVVQLDMAAGAGKQYLIKDVQYDHLQKDLVHVDLMRVDVHERVEVK
                     VPVSLKGEAKGIHEGGELVQLITDLEVECPLLSIPELITHNVKELGVDDNLYVRDLTL
                     PPDVKALHEPDEIVAVVRMKKAEEVAPAAAAAEGAEAAAEPEVIKRAKEEEAPGEEKK
                     "
     gene            3045..3677
                     /gene="pth"
                     /locus_tag="PLU99_10300"
     CDS             3045..3677
                     /gene="pth"
                     /locus_tag="PLU99_10300"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOO17487.1"
                     /translation="MKLIAGLGNPGKRYAESRHNVGFRVIEELARRWGVDEARYDRHF
                     EALVGQAQRAGEPVLLMQPQTYMNESGRSVAAAWRFYKLALSELLLVLDDLDLPVGRL
                     RLRPGGSSGGHKGLTDVIRCLGSEDFARLRIGIGKVHPSATVEYVLSRFEPDEQPAIA
                     AAITKAADAVEVWLRQGIDAAMNEFNRKGDGPARSATPPAKERPAKGDES"
     gene            3674..4165
                     /gene="rpsF"
                     /locus_tag="PLU99_10305"
     CDS             3674..4165
                     /gene="rpsF"
                     /locus_tag="PLU99_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOO17488.1"
                     /translation="MKRYEGMFLFDSAGARDWAAIDQEVRRLLGRIGAEPLVCVKFDE
                     RKLAYEIKRRKRGTYVLTYFNAPPERITELERDAQLSESVLRMMVLRAEHVTDERIRE
                     LQAWPPENPLQPIGEGRRHDDGDRGRGGRDWERRGPREREPLAEPAEEEAPEAPDLDL
                     ARE"
     gene            4220..4696
                     /gene="ssb"
                     /locus_tag="PLU99_10310"
     CDS             4220..4696
                     /gene="ssb"
                     /locus_tag="PLU99_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOO17489.1"
                     /translation="MASFNKVILMGNLTRDPQLRYLPSNMAVCDFGVAVSRNWRDKDG
                     NLKEEVCFVDVTAWARQAETINQYMRKGRPILIEGRLKLDQWTGQDGQKRSKLSVVVE
                     SFQFVGPREGGPASSGPPGGAPYQQAEPPAPERPSGYGAPLPPEEDLPPPSDNIPF"
     gene            4718..4975
                     /gene="rpsR"
                     /locus_tag="PLU99_10315"
     CDS             4718..4975
                     /gene="rpsR"
                     /locus_tag="PLU99_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOO17490.1"
                     /translation="MAMRRMLATKKKKKPRRSSEPSRYRGVQVKLEEIDYKNVALLQR
                     LTSAQGKLFSRKRTGLSAEAQRKVSLALKRARQMGLMPFIT"
     gene            5084..5593
                     /gene="rplI"
                     /locus_tag="PLU99_10320"
     CDS             5084..5593
                     /gene="rplI"
                     /locus_tag="PLU99_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOO17491.1"
                     /translation="MRLLLLKDIRKLGNLGDVVEVTTGYARNYLIPQRLATEPTEENI
                     KAIEQEKKRAAAERAARLKVFTDLAEKMKDVQVTIEAAANPEGTLYGSVGRKEVADAL
                     HALGFAVRAEQIELDPPIRTLDNRTVTIRFTDELSTDIKVWVVKEGGAEHGEHAEEQG
                     AAASRTEPA"
     gene            5538..7013
                     /gene="dnaB"
                     /locus_tag="PLU99_10325"
     CDS             5538..7013
                     /gene="dnaB"
                     /locus_tag="PLU99_10325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOO17492.1"
                     /translation="MANTRRSREQPPREPSRPDSRPFFDRALPHDDDAERALLGSMLL
                     EKEAIGEAITVLRDAGAAALDSPRNQRLFEVLVALYVENRPIDGVVLREELTRRGWWE
                     DLGGHEYLVGLVNAVPSALRVRYYAQIVLEKYLLRELIGATHKVMEAAYADERPAKET
                     LDYAEKEIFAVTERRVSGEAAPLDQLMRDVIQTLKSQGEGVLTGEPTGFIKLDELTCG
                     LQPAELIIVAGRPSMGKTALGLNIAEHLAIVENRPVLFFSLEMSRQQVAQRVLCSQAR
                     VNAHQLRRQRRSAADLRLLEDFAQRLEAAPLYVDDTSDLSILEMRARARMAYRKHKIR
                     AVFVDYLQLMHVPGSESRQVEVATISRGLKALAKDLNVPVVAMAQLNRNPEDKTRKGN
                     RPRMSDLRESGAIEQDADVIALLHRESYYKSPTVGEDEEAGGVPDPEENLADLIIAKN
                     RNGPVGDIKLHFSREYTRFDNHDPRPREPYYTPAGSQETPF"
     gene            7714..8604
                     /locus_tag="PLU99_10330"
     CDS             7714..8604
                     /locus_tag="PLU99_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO17493.1"
                     /translation="MSPTERRLLHQTLSETMECVWNPVFRKMAAANQPLTPLPELQSI
                     SVPSDDGLDLLTPPRHKVLTADEERDLFIRFNYCRYRMMCIVHDHEGRRLGLEAAREL
                     LHWADEALKTRNHIVRANTSLVMAMARRTKTMGVELGDLVSEGNLALLRCVDKFDCAR
                     GFKFSTYACRAIMSGFARLGAKSARYRTQFPAPFDPTFERSDFLEQQRAGEADDCVTG
                     LKLILSHNTADLSRVEQRVISARFALDRAGRKEQKGRPRTLEQVGALLGVSKERVRQI
                     QNRALQKLRNVIDETVLAAS"
BASE COUNT         1662 a         2668 c         2869 g         1461 t
ORIGIN      
        1 ggaacgacta cattgccgtc tctgggcttc gtcccggagt ttacgcccgt gagcggggca
       61 aatgggtctg aatggcccat ggtgggtcgg cggccacgcg accgtcggct caggcctcag
      121 agcgggtccg cgaatcgcgc attcgcttaa gtccgtgctg cgccctttaa ccctggcgtt
      181 tggcgtgggt tgggctaccg agcctcggcg tttacgccgg gatcgtgaac cgtggctgat
      241 cgtttgcccg gtggtgtaat tggtaacaca ccaggttttg gtcctggcat tcctggttcg
      301 agtccaggcc gggcaggttt gatgcagcaa tgcaggcttg aatgcagaaa cttaggattg
      361 aaagccaagc atgacggtcg gaaccggtga aaagacgacg cccaccgctc cccgcgtcgg
      421 cgcggtaatc ctcgccgccg gtaagggcac gcggctcaag accgagctgc ccaaggtgct
      481 gcacgaggtc tgcgggcggc cgatgctggc ctacgtcatc gacgcctgcc gcgcggccgg
      541 cgtcaaggag tgcgtcgtcg tcgtcggcta ccagaaggat ctggtgattc gagccttcac
      601 cgaggatcgc gacctgacct gggtcgagca gaacccgcag ctcggtaccg gccatgccgt
      661 catggtttgc cgcgagcacc tggccgggcg gttcgaccac ctatttgtgc tggccggcga
      721 tgggccgctg attcgggccg agacgctgcg aacgctgatc gaacgccacc tggccgaaca
      781 ggccgccgtc acgctggcga ccgcggtctt ggacgacccg accggctacg gccgcatttg
      841 gcgcgatgcg gagggccgcc ttctggggat cgtcgagcat ggcgacgcca cgccggagca
      901 gaagcgaatc cacgaggtca acccgagcta ctactgcttc aaacagccgg aattgctggc
      961 ggcgctggac caggtgcggc cgaacaacgt gaagaacgaa tactacatca cggacgcgat
     1021 ctcgatcctg ctggcgggcg ggcataaggt gcaggcgatc acctcggtgc cgccgcagga
     1081 catcttcagc atcaactcgc gccaggaact ggcgatggtg aacgcggtga tgcgcgaccg
     1141 cattctcgac cggctgatgt cctcgggcgt gactatagtt gatccgcgaa ccacgtggat
     1201 cgacgaccgg gccactattg gccaggatac agttattcac cctaacgtcg tcatcagcgg
     1261 cccggccatc attggacgcg gttgccgcat cggtccgttc gtgaacttga ccggcgccgc
     1321 gcgtgtgccg gaccacgggg tgatttcgcc gttcgccgga ggcgtggcat gagtgatatg
     1381 cggatttttt cggggcgcag caacccggcc atggcgcaga gcatttgcca gtatctgggc
     1441 ctgccgctgg gccgcgcgca gcttgacaac ttccccgacg gcgagattca gctcaagctg
     1501 ttcgaggacg tgcgggggcg cgacacgtac gttatccagt ccacgtgctc tccggtgaac
     1561 gacaacctgc tggaactgct gatcttcatc gattgtttga agcgggcgtc ggccgaacgc
     1621 atcacggccg tgattcccta tttcggctac gcccggcagg accgcaagga cgagggccgc
     1681 gtgccgatca cggccaagct ggtagcgaac attctgaccg aggcgggggc cgaccgggtt
     1741 ctgacggttg agctgcacgc cgcccagatt cagggcttct ttgacattcc ggtcgacaac
     1801 atgtcggccc agccggtgtt tcaggcttat ttcgaaacac tggatttgca gccgctgacg
     1861 ctggtgagcc cggacgtagg caatgccaag catgcccgcg tttacgctga ccggatgggg
     1921 gccaacctgg cgatcatcga caagcgccgc atcagcggca gcgaggcggc cccgttggcg
     1981 ctgatcggca acgtagccga ccagaacgtg ctgatgatcg acgacatcat cgccaccgcg
     2041 ggcacggtgg tacaggcggc caagttggtg cggcagcacg gcgcgcggcg gatcgtagtc
     2101 gccgccacgc acggcgtgct gtgcgggccg gcgatcgagc gcctggccgg cgcaccgatc
     2161 gatcacattg ccgtgaccga caccattccc attccggagc aggtgcgggc gcggctaccg
     2221 aacctgagtg ttttgacggt aagcgagctg atcggcgagg cagtccgccg cattcatcgg
     2281 catgagtccg tgagcagtct gtttatgaag tagttgtcgg tgttccggcg tcagtctgcg
     2341 acccggactg acgactgaag accgaccggt aaaaactgaa agaggtaagt tatggagatt
     2401 gcaaaaatcc gcggcgaggc gcgccagccc ggcaatcgtc acgcgaacga gcgcttgcgg
     2461 cggcgtggcc tgattccggc tgtcatttac gggcacggcc aggcgccgga gacggtggcc
     2521 gtttcgcagc acgacctgga gttggtgttg ggccgggcgc acgtggtgca gctcgacatg
     2581 gcggctggtg cgggcaagca gtacctcatc aaggacgtgc aatatgacca cctgcagaag
     2641 gacttggtac acgtggacct gatgcgggtc gacgtgcatg agcgggtcga ggtcaaggtg
     2701 ccggtgtcgc tcaagggcga ggccaagggc attcacgaag gcggcgagct ggtgcagctc
     2761 atcaccgatc tggaggtcga gtgcccgctg ctgagcatcc cggagttgat cacgcacaac
     2821 gtcaaggagc ttggcgttga cgacaacctg tacgtgcgcg acctaaccct gccgccggac
     2881 gtcaaggccc tgcatgagcc cgacgagatc gtcgccgtgg tgcgcatgaa gaaggccgaa
     2941 gaggtggccc cggccgccgc ggccgccgaa ggcgcggaag ccgccgccga accggaagtc
     3001 atcaagcgtg ccaaggaaga agaggcgccg ggcgaggaga agaagtgaag ttgatcgccg
     3061 gcctgggcaa tccgggcaag cgctacgccg agtcacgcca caacgtcggc tttcgcgtca
     3121 tcgaagagct ggcgcgacgc tggggtgtgg acgaagcgcg ctacgaccgg cacttcgaag
     3181 cgctggtcgg tcaggctcaa cgggccggcg agcctgtgct gctcatgcag ccgcagacct
     3241 acatgaacga aagcgggcgc agcgtggccg ccgcctggcg gttctataag ctggcccttt
     3301 ccgaattgct gctggtgctg gatgaccttg atctgccggt ggggcgcttg cggctgcggc
     3361 cgggcggttc gtccggcggg cacaagggcc tgacggacgt gatccgctgc ctgggcagcg
     3421 aagacttcgc acgcttacgc atcggcatcg gcaaggtcca cccctcggcc acggtcgaat
     3481 acgtgctgag ccggttcgag ccggacgagc agcccgcgat agcggccgcc atcaccaagg
     3541 ccgcggacgc ggttgaagtc tggctgcggc agggcatcga cgcggctatg aacgagttca
     3601 atcgtaaggg cgacgggccc gcgcggtccg cgacgccgcc ggccaaagaa cggccggcca
     3661 aaggagacga gtcttgaaac gttacgaagg tatgttcctg tttgacagcg ccggcgcccg
     3721 cgactgggcg gcgatcgacc aggaagtgcg ccgcctgttg gggcgtatcg gggccgagcc
     3781 gctggtgtgc gtcaaatttg acgagcgcaa gctggcctac gagatcaagc ggcgcaagcg
     3841 tggcacgtac gtgctgacgt acttcaacgc cccgcccgag cgcatcaccg agttggagcg
     3901 cgacgcgcag ctcagcgagt cggtgctgcg gatgatggtg ctgcgggccg agcacgtcac
     3961 ggacgagcgt atccgcgaac tgcaggcctg gccgcccgag aaccccctgc agccgatcgg
     4021 cgaaggccgc cggcacgacg acggcgaccg cgggcgcggc gggcgcgatt gggaacggcg
     4081 cggtccgcgc gagcgcgaac cgctggccga gccggctgaa gaagaggcgc cggaagcacc
     4141 ggatctggat ctggcacgcg agtagcgcgc cagtgcggat gtgacgtact gacgtactga
     4201 tgaccctgag ggcccgatca tggctagctt caacaaagtc atcctgatgg gcaacctgac
     4261 acgcgacccg cagctacgct acttgcccag caacatggcc gtctgcgact tcggcgtcgc
     4321 cgtgagtcgc aactggcgcg ataaagacgg caaccttaag gaagaagtgt gcttcgttga
     4381 cgtaactgcc tgggcgcgtc aggccgagac catcaaccag tacatgcgaa aggggcgccc
     4441 cattctgatc gaaggccggc tgaagctcga tcagtggacc ggccaggacg gtcagaagcg
     4501 ctcgaagctg agcgtggtag tggaaagctt ccagttcgtc gggccgcgtg agggtggtcc
     4561 ggcatcttcc ggcccgccgg gcggagcgcc ctaccagcag gccgagccgc cggccccgga
     4621 gcggccaagc ggttacggcg cgccgctgcc gcctgaagag gacctgcccc cgccctcaga
     4681 caatattcca ttttgacaac gccattcgga gtagatcatg gcaatgagac gaatgctcgc
     4741 aaccaagaaa aagaagaagc cacgtcgcag ctcggaaccc agccgctatc gcggcgtgca
     4801 ggtcaaactc gaagagatcg actataagaa cgtggccctt ctgcagcgcc tcaccagcgc
     4861 ccagggcaaa ttgttctcgc gcaaacgcac cgggctgtca gccgaggcgc agcgtaaagt
     4921 gtcgctggcc ctcaaacgcg cccggcagat gggcctgatg ccgttcatta cgtgagactg
     4981 tggctcaggc ggtgggaccg gttttcaacc ggtcatgacc ggttgaaaac cggtcccaca
     5041 cagacggtcc tacgcggacg gtcccacaag gagatttcag aaaatgcgtc tgctgttgct
     5101 gaaagatatt cgtaaactgg ggaatctcgg cgacgtcgtg gaggtcacga ccggctacgc
     5161 gcgcaactac ttgatcccac agcgcctggc caccgaaccc accgaggaaa acatcaaggc
     5221 catcgagcag gagaagaaac gcgccgcggc cgaacgggcc gcgcgcctga aagtcttcac
     5281 cgacctggcc gagaaaatga aggacgtgca ggtcaccatc gaggccgccg ccaaccccga
     5341 aggcacgctc tacggctccg tgggccgcaa ggaagtcgcc gacgcgctgc acgcgctggg
     5401 ctttgccgtg cgggcggagc agattgagct tgatccgccc atccgcacgc tggataatcg
     5461 caccgtgacc attcggttca cggacgagct ttcgaccgac atcaaggtct gggtcgtcaa
     5521 agaaggtggc gcggagcatg gcgaacacgc ggaggagcag ggagcagccg cctcgagaac
     5581 cgagccggcc tgattcacgg ccttttttcg accgggcact gccgcacgat gacgacgcgg
     5641 agcgggccct gctcggttcg atgctgctgg aaaaggaagc catcggcgag gcgattaccg
     5701 tcctgcgcga cgccggcgcc gcggccctgg attcaccccg caaccagcgc ctgttcgagg
     5761 tgctggttgc gctttatgtc gagaaccggc cgattgacgg ggtggtctta cgtgaggagc
     5821 tgacgcgccg cgggtggtgg gaggacttgg gggggcacga atacctggtg gggctggtca
     5881 acgcggtgcc ttcggcgctg cgcgtccgct attacgccca gatcgttctg gagaaatacc
     5941 tgctgcgtga gctgatcggc gccacgcaca aggtgatgga ggccgcctac gccgacgaac
     6001 ggcccgccaa ggagacgctc gattacgccg agaaggaaat cttcgccgtc acggagcggc
     6061 gcgtttcggg cgaggccgca ccgctcgacc agctcatgcg cgacgtcatc cagacgctca
     6121 aaagccaggg cgagggtgtt ctgaccggcg agccaaccgg gttcataaag ctcgacgagc
     6181 tcacctgcgg cctccagccg gccgagttga tcatcgtggc cggccgtcca tcaatgggca
     6241 aaacggcgct gggactgaac attgccgagc atctggccat cgttgagaac cggccggtgt
     6301 tgttcttcag tctggagatg agtcgccaac aggtcgcgca gcgcgtgctg tgcagccagg
     6361 cgcgggtcaa tgcgcaccag ttgcggcgtc agcggcgttc ggccgccgac ctgcgcttgt
     6421 tggaagactt tgcgcagcgc ctggaagcgg ccccgcttta tgtggatgac acgtcggatt
     6481 tgtccattct cgaaatgcgc gcccgggcgc gcatggcgta ccgtaagcac aagattcgcg
     6541 cggtgttcgt ggattacctg caactgatgc acgttcccgg ctccgagagc cgccaggtcg
     6601 aggtggccac catctcgcgt gggctcaagg cgctcgccaa ggacctcaac gtaccggtcg
     6661 tcgcgatggc tcagctcaac cgtaatccgg aggacaagac tcgcaagggc aaccgcccgc
     6721 gcatgagcga cctgcgcgaa tccggcgcca tcgagcaaga cgccgacgtc attgcgctgc
     6781 tgcaccgcga gtcctactac aagtccccga cggtcggcga ggacgaggaa gcgggcgggg
     6841 ttccggaccc cgaagagaac ctggccgacc tcattatcgc caagaaccgc aacggcccgg
     6901 tgggcgacat caagctccat ttctcgcgcg agtacacccg cttcgacaac cacgacccgc
     6961 gtccgcgcga accctactac acgccggccg gcagccagga gacgccgttc tgacgccggc
     7021 ctgaccgcac cgctcacccc gctcctctga gcggaccatc gggcgtgagg cgtcctgccc
     7081 aaactacgcc cgtttggcgt catttcacct gtgacgggtg agaaggtaag gacggcgact
     7141 taccacgttc ccacattccc acgttcccac gttcccactt tcccacaacc gacctgcaga
     7201 tcaccagaca tgcgccaggt ttgggcgagg ctttgttccg tctgaccaac atccttcggc
     7261 tcgcgggaac aaaacgggca agaaatcggt tttatattcg tcttattatg gggcttcggt
     7321 acggcggtgg agtgcgggag ccccggccgg gattacggca attttatgtt caatttatcc
     7381 cgttttcaga ggtgcggtca atagtattcg gtggcgcggc cggttggagg tttgggccac
     7441 cgggcgtaac gagcgcgagg caggcataag caggaggcgt ttttcccgcc agtggcccac
     7501 gctcgaaaga attcgcttgc caaaagaagg aattagtact attttagagc gaattgatgg
     7561 aactgatttg ttgtggcggg cgtagaactc gtgtggtttg ggttgagccc gcccggcacg
     7621 ggcaaacgtg atatggaaaa gctaaccaga acatcgaatc tgcgaaaact ccgccccagg
     7681 gcgaagtccc cgacagtcgc ggcactgcag cgcttgtcgc ctaccgaacg gcggctcctg
     7741 caccagacgc tttcggaaac catggaatgc gtctggaacc cggtcttccg caaaatggcg
     7801 gccgccaacc agcccctgac tccgctcccc gagcttcaat ccatctcggt gccgagcgac
     7861 gacgggctgg atcttctgac gccgccgcgg cacaaagtgc tcacggccga tgaagagcgc
     7921 gacctcttca ttcgcttcaa ctactgtcgc taccgcatga tgtgcatcgt gcacgatcat
     7981 gaaggccgcc ggctggggct ggaagcggcc cgggagcttc tgcactgggc tgatgaagcc
     8041 ctcaagacgc gcaatcacat cgtgcgggcg aatacgtcgc tggtcatggc gatggcccgc
     8101 cgaaccaaga ccatgggcgt tgagctcggc gatttggtca gcgagggcaa cttggccctg
     8161 ttgcggtgcg tcgacaagtt cgactgtgcc cgcggcttca agttcagcac ctacgcgtgc
     8221 cgcgcgatca tgagcggctt cgcccggctg ggcgccaagt ccgcccgcta ccgcacgcag
     8281 ttcccggcgc ccttcgaccc cacgttcgag cggagcgact ttctggagca gcagcgggcc
     8341 ggcgaggccg acgattgtgt caccggcctg aagctgattc tgtcgcacaa cacggccgac
     8401 ctgagccgtg tcgagcagcg cgttatcagc gcccgcttcg ctctggaccg ggccggtcga
     8461 aaagagcaaa agggccggcc gcgtacgctc gagcaggtgg gcgctctgtt gggcgttagc
     8521 aaggagcgcg tgcggcagat tcaaaaccgc gccctgcaaa agctgcggaa cgtgatagat
     8581 gaaacggtgc tggcggcttc ttgagtaagc ggcacgttcc gcttgcgccc gcgctagccc
     8641 agagcggcgc gttggacccg
//
LOCUS       DAOSYS010000157         8595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7011_length_8595_cov_4.901522, whole genome shotgun sequence.
ACCESSION   DAOSYS010000157 DAOSYS010000000
VERSION     DAOSYS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7011_length_8595_cov_4.901522"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1226)
                     /locus_tag="PLU99_10335"
     CDS             complement(30..1226)
                     /locus_tag="PLU99_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HOO17494.1"
                     /translation="MTPLNIRNVRIFRLAIPMRLRFDHAAAVREVADPIVLQISAAAP
                     YAHYVGYGETLARTYVTGESAGSVVQDIADLFVPRLASFAPRSFAEALECIEALPTQF
                     SGRIVTAARAAVELALLDLAGKVFRRRPADVAGWMGLTGFGAPGCLSTARYSGIVVGR
                     TRRRLTALLHLQRWYGLRDFKLKVAIEGWEQRLEWAYSVLRGSIDRGQVSLRVDANSG
                     WTLSEAEDALPLLEENGVTAIEQPLSDHDDARLPDLAVQTKCDLIADESLITLNDAQR
                     LIDGGGVRVFNIRLAKNGGLLPALRIARLALSAGVNVQLGCLVGETSILSAAAVGFLE
                     TCPAVRFVEGAFGKLLLKEDVVRRPIRFGRRGRIAPRPDFGLGIEVDSRAVEHLAVER
                     GRSIPL"
     gene            complement(1233..2078)
                     /locus_tag="PLU99_10340"
     CDS             complement(1233..2078)
                     /locus_tag="PLU99_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOO17495.1"
                     /translation="MILALSGCRLGSVSPTARLRITGAQIATELVDSWLRDAREYRFV
                     VEQVRAPSWSQVGFQALARGECDLAVTDRPLEERERELFGDQEVRGLRVGFYGYALYV
                     NPANPLDSIFAKHITLLFQRKMRDWRELGGNEFPLEGPIHLYGPKKVTRGGNVLMQQA
                     RIWFAEPTWEACDSDAEIINRVAEDPLGLGFASVGLDGRVRYLGIRMRRDGPPVFPSL
                     EDIEADRYGLAKVIYVYFVEPPSPAVNAAIEYLFSADGQRAIEETDAWQVPWERAAVI
                     PGNER"
     gene            2229..3740
                     /locus_tag="PLU99_10345"
     CDS             2229..3740
                     /locus_tag="PLU99_10345"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOO17496.1"
                     /translation="MPTKTTIDQLANHIGQTVTLQGWLYNSRSSGKLLFLIVRDGTGL
                     CQCVLEKSPEQAELFASAEKLGQEASLEVTGLVRADPRSPGGHELTVSAVNVIQDVEG
                     YPITPKEHGIEFLMQRRHLWFRSQRQWAILRVRATVVDAIRRYFNDHGFVLIDTPIFA
                     PAAGEGSQTLFKVDYFGENVYLAQTGQLYLEAACMAHRRVYCFGPTFRAEKSKTRRHL
                     TEFWMVEPEIAYADVDEVIAVAEDFVHEVVQTVLRERRPELEMLNRDVAKLEASTQKP
                     FYRITYSEAAELLHSDRVRDFLLGDLEAAKARVAEINKLIAEKEAERTAPGTKKWKAE
                     KLAQEVLDLREELAEREEDVRNIPHHMELAANFEWGSDLGGSDETIIARLHDRPVFVT
                     HYPRGCKAFYMKRNEQDHRVVNNLDLLAPEGYGEIIGGSQREDDLEVLRERMKEEGLK
                     EEDYAWYLDLRRYGTVPHGGFGLGVERTVAWICGLKHIRETIPYPRMMGKIYP"
     gene            complement(3780..5405)
                     /locus_tag="PLU99_10350"
     CDS             complement(3780..5405)
                     /locus_tag="PLU99_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOO17497.1"
                     /translation="MRWLQFFAIVLAGMGMGARAGQTVVCQGRSCGGAGPRAYAYVVE
                     AAVEPILEFAVSTNDLEPGNYTHVLVPPGWQFAVEAVGMEHSCGGYAPDGGFSLGRCA
                     TLSLGRVRWWTDDPMSAVESFTFGFDHDWLAEDAGWVVHDEEGPGSVTAWLSPVGMGT
                     GPVHAPGGSPPNLRLGPEEFVALRDGTEITVPGYSVPSFVRWDGDDLPDLVVGEGGGT
                     PGKVRVYLNTGTRGQPAFDTFFYAQSQGVDLSVAASGCQGAFPRVVYWDGDDRKDLLI
                     GMASGRVRLYLNVGSDEAPAFDGGAYLQVGLPGEKVDIRVAARATPSAVDWNNDGRKD
                     LVVGSYDGSVYIYLNEGTDGAPDFRAETQAQMGGTLLMVPGSRSSPDVADLDGDGKKD
                     LLVGNHAGQLFFFSNTGTDADPSFTTYIMVRSAGLAIDLEGVPRSRPAVCDWNDDGLP
                     DVLLGSGDGKVRLYRNHYAVGDLNCDAAVNVFDIDPFVLALTSAPEFGAYLAAYPQCE
                     GMLADTNCDGQVNVFDIDPFVDCLVGAASPSCP"
     gene            complement(5524..7173)
                     /locus_tag="PLU99_10355"
     CDS             complement(5524..7173)
                     /locus_tag="PLU99_10355"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOO17498.1"
                     /translation="MKIAAVQLNPTIGDVEGNTARILDGFARATSAGAELVVLPEQSI
                     LGYPAKDLLLRREVIEQNLAALQRIVAATAGLQAGVIVGFAEPNEGGIGRPVFNSAAF
                     IADGQIVARSRKRLLPTYDIFDEARYFEPGGVQPVIDFRGRRVGVMICEDMWSTQQLL
                     ARPLYDIDPPCDLAAAGADLLINISASPYYLGKHESRLELMAGHARRYGTPLLFCNQV
                     GGNDELLFDGLSSVVDATGRLVAQAKAFEEDLLLIDLDDLDSARLEAMPTGVANLRAA
                     LVMGLRDYMRKCRFTGVVVGLSGGLDSAVVACLAAEALGPPNVLGVAMPSRYSSEHSV
                     ADAKALAENLGIDFSIIPIEPLHAAFERELRPHFAGRPPDVTEENIQARIRGVSLMAL
                     SNKFGRLLLTTGNKSELAVGYCTLYGDMCGGLALISDVPKTMVYELAHEINRRAGREV
                     VPRSTLTKPPSAELRPNQTDQDSLPPYEVLDEILARYEERLESIEQIVAAGFDSAMVT
                     EVVRKIHLNEYKRQQAAPGLKVTSRAFGFGRRMPIAARLAF"
     gene            7872..>8595
                     /locus_tag="PLU99_10360"
     CDS             7872..>8595
                     /locus_tag="PLU99_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17499.1"
                     /translation="MTYASEYNTRHNIPSDITMLVGASEVDPPTSPPTYEVQISLTLD
                     PTASARTVRVYAVQVLDYYPTSPSYSRNCMRTWTSTEDVALVPGQTVLITKMLQIDTV
                     SAANPDNMTLIAWAQVPNSSAPAEVYQAAMINYPFEALYPIKITLPEGVPEFIPPDED
                     TALTVRIQNGGEDLLPDSPLMHYRYDDGAFQTAPLVPFGGEYFTATLPAPGCGAEPEF
                     YFSAQGDGGSTVIYPENAPAEVV"
BASE COUNT         1616 a         2928 c         2616 g         1435 t
ORIGIN      
        1 tccggcgagc gccgtcgcca atcggcacgc tacagcggga tagaccgtcc gcgctccacg
       61 gccagatgct ccacggcacg cgaatcgacc tcgatcccca ggccgaagtc cgggcgcggc
      121 gcgattcgtc cgcggcggcc aaaacggatt ggtcgccgca ccacgtcttc cttcagcaga
      181 agcttcccga aggccccctc cacgaaccgc acggccgggc aggtttccag gaagccgact
      241 gccgcggcgc tgaggatgct tgtttcgccc accaggcagc caagctgcac gttcaccccg
      301 gcggatagcg ccagccgggc gatgcgcagc gccggcagca agccgccgtt ctttgccagg
      361 cggatgttga agacccgcac accgccgccg tcgatcagcc gctgcgcgtc gttgagcgta
      421 atcagcgact cgtcggcaat caggtcgcac ttggtctgca cggccaggtc cggcaggcgt
      481 gcatcatcat ggtcgctgag cggctgctcg atcgcggtca cgccgttctc ttccaaaagc
      541 ggcagggcgt cctccgcttc ggaaagcgtc cagccgctgt tggcgtcgac gcgcaggcta
      601 acctggccgc ggtcgatcga tccgcgcaag acgctatagg cccattccag ccgctgctcc
      661 cagccctcga tcgccacttt cagcttgaaa tcccgcagtc cgtaccatct ctgaagatgc
      721 agcagggcgg ttaaccggcg gcgcgtgcga ccaaccacga ttcccgagta acgggccgta
      781 gacaaacacc ccggcgctcc gaaaccggtc agacccatcc agccggccac gtccgccggc
      841 cgccggcgga acaccttccc tgccaggtcc agcagggcca gctccaccgc cgcccgcgcc
      901 gccgtaacga tccgccctga aaactgcgtt ggcagagcct cgatgcactc gagcgcctcg
      961 gcaaacgatc gcggggcgaa gctggccagc cgcggcacga acaggtcggc gatgtcctgc
     1021 acgaccgagc cggcgctctc ccccgtcacg tatgttcgcg ccaacgtctc accgtagccg
     1081 acgtagtggg cgtacggggc ggcggccgaa atttgcaaaa caatcggatc cgccacttcg
     1141 cgaacggccg cagcatgatc aaaccgcaag cgcatcggaa tcgccaggcg gaagatgcgc
     1201 acgttacgta tattgagcgg cgtcactgca agttaccgtt cgttgccagg aatcactgcc
     1261 gcacgttccc acggcacctg ccaggcatct gtttcctcga ttgcccgctg cccatccgca
     1321 ctgaacaggt actcgatggc ggcattcacg gccggactgg gcggctcgac gaagtaaacg
     1381 taaatcacct tggcaagacc gtagcggtcg gcctcgatat cttcgagcga tggaaaaacg
     1441 ggcgggccgt cgcgtcgcat gcggatgccc agatagcgca cgcggccgtc caacccgacg
     1501 ctggcaaatc ccagccccag cggatcctca gccacacggt taatgatctc cgcgtcgctg
     1561 tcgcaggctt cccatgtggg ctcggcaaac cagatgcggg cctgctgcat cagaacgttg
     1621 ccgccgcgcg tgactttctt cgggccgtac aggtgaatcg ggccctccag ggggaactcg
     1681 tttccgccca gctctcgcca atccctcatc ttgcgctgga agagcagcgt gatgtgcttg
     1741 gcgaagatgc tgtccagcgg attagccggg ttgacgtaca gggcataccc gtaaaagccg
     1801 acgcgcaacc cgcgcacctc ctgatcgccg aacagctcgc gctcgcgttc ttccagcggg
     1861 cgatcggtga cggccaggtc acactcgccg cgtgccaacg cctggaaccc aacctgcgac
     1921 caactcgggg cccgcacctg ctcgacgacg aagcggtatt cgcgcgcgtc gcgcagccag
     1981 gaatccacca gctccgtcgc aatctgagct ccggtaatcc gcagccgtgc cgtcggcgaa
     2041 accgatccca gccggcagcc gctcagtgcc aggatcaaaa gcccgccggc tgcccattgg
     2101 ccaaaccccc tgttgtcacc taaactggca tgtttcgtgc atgctcgcga cactaggcgg
     2161 gcgcgaggcg ggcaatggga ccacgagcgc acccgggcga atgaggaagc ttgaaaggaa
     2221 cggcctgaat gccgaccaag acgaccatcg atcaactggc taaccatatc ggccaaacgg
     2281 taacgctcca gggttggctc tacaacagtc gctcgtcggg caagctcctc ttcctgatcg
     2341 tgcgggatgg caccggcctg tgccagtgcg tgctggaaaa gtcgccagag caggccgaac
     2401 tcttcgccag tgccgaaaag ctggggcagg aagcttcgct cgaagtgacc ggccttgtcc
     2461 gggccgaccc gcgcagcccc ggcgggcacg agttgaccgt ttcagccgtg aacgtcatcc
     2521 aggacgtcga gggctacccg atcacgccca aggagcacgg gatcgagttc ctcatgcagc
     2581 ggcggcacct gtggttccgt tcgcagcgcc agtgggccat cctgcgcgtg cgggccaccg
     2641 tggtcgacgc gatacgccgt tacttcaacg atcacggctt cgtgctgatc gatacgccga
     2701 tcttcgcccc ggccgccggc gagggctcgc aaacgctctt caaagtggac tacttcggcg
     2761 agaacgtcta tctggcccag accggccagc tttaccttga agcggcctgc atggcccatc
     2821 gccgggtcta ctgcttcggc ccgacgtttc gcgcggagaa gagcaagacg cgccggcacc
     2881 tgaccgagtt ctggatggtt gagccggaaa tcgcgtacgc cgacgtcgac gaagtaatcg
     2941 cggtcgcgga ggacttcgtt cacgaggtgg tgcaaaccgt cttgcgcgag cgccgccccg
     3001 agctggaaat gctcaatcgc gacgtggcca agctcgaagc ctcgacgcaa aagccgttct
     3061 accgcatcac ctacagcgag gcggccgaac tgctgcacag cgaccgcgtg cgcgacttcc
     3121 tgctgggcga cctcgaagcg gccaaagccc gcgtggccga gatcaacaag ctcatcgcgg
     3181 agaaggaggc ggaacggacc gcgccgggca cgaagaagtg gaaggcggag aagctggccc
     3241 aggaagtgct cgacctgcgt gaagagctgg ccgagcgcga agaagacgtg cgcaacattc
     3301 cgcaccacat ggagctggcc gcgaatttcg agtggggctc cgatctcggc ggctcggacg
     3361 aaaccatcat cgcccgcctg cacgaccgcc cggttttcgt cacgcactat ccgcgcggct
     3421 gcaaggcgtt ttacatgaag cgcaacgagc aggaccaccg cgtggtgaac aacctcgacc
     3481 tgctggcccc ggaaggctac ggcgagatca tcggcggcag ccagcgtgag gacgatctgg
     3541 aagtgttgcg cgagcggatg aaggaagagg gccttaagga agaagactac gcctggtacc
     3601 ttgacctacg ccgctacggc accgtcccgc acggcggctt tggcctgggc gtggagcgca
     3661 cggtggcgtg gatctgcggc ctgaagcaca tccgcgagac aattccatac ccgcgcatga
     3721 tgggcaagat ttacccgtag ctggcgaaag ccggccgcgt cttgaacgct aatccgcgct
     3781 cacgggcagg acggagacgc ggcgccgacc aggcagtcca cgaacgggtc gatatcaaac
     3841 acatttacct gcccgtcgca attcgtgtct gccagcatgc cttcgcactg cggatacgcc
     3901 gccaggtagg ccccgaactc cggcgcgctg gtcaacgcaa gcacgaacgg atcaatgtcg
     3961 aacacattga ccgccgcgtc acaattcaaa tcgcctaccg catagtggtt gcggtacagt
     4021 cgcacctttc catcgccgct gcccagcagt acatccggca aaccgtcgtc attccagtcg
     4081 cacaccgccg gccgcgagcg cggcacgccc tccagatcga tcgccaggcc ggccgagcga
     4141 accatgatgt acgtggtgaa gctcggatcc gcgtcggttc cggtgttgct gaagaagaag
     4201 agctgcccgg cgtggttccc gacgagcaaa tccttcttgc cgtcgccatc caggtcggcc
     4261 acgtcgggac tggagcgcga gcccggtacc atcagcagcg tcccgcccat ttgggcctga
     4321 gtctcggccc ggaaatcagg tgcgccatcc gtgccctcat tgaggtagat gtaaacgctg
     4381 ccatcatacg aacccaccac caggtccttc ctcccatcgt tgttccagtc cacggccgat
     4441 ggcgtggctc tggcagcgac gcgtatgtcg accttctcgc ccggcaggcc gacctgcagg
     4501 taggcgccgc cgtcaaaggc cggcgcctcg tccgagccga cgttcaggta tagcctgact
     4561 cgcccggacg ccatgccaat aagcaaatct ttccggtcat ctccgtccca gtagaccacg
     4621 cgcggaaacg ctccctggca gcctgaagcc gccaccgaca gatccacgcc ctgcgactgc
     4681 gcgtagaaga acgtatcgaa cgccggctgc ccgcgcgtgc cggtgttcag gtacacacgc
     4741 accttgcccg gcgtaccgcc gccctcgccg accaccaggt ccggcaggtc atcgccatcc
     4801 catcgcacga acgagggcac cgaatacccc ggtaccgtta tctcagtgcc atcgcgcaaa
     4861 gccacgaatt cctccggccc gagccgaagg tttggcggcg aaccgccggg tgcgtgtacc
     4921 gggcccgtgc ccattccgac cggcgatagc cacgctgtga cggaacctgg cccctcttcg
     4981 tcgtgtacca cccagcccgc gtcttccgcc agccagtcgt gatcgaagcc gaatgtgaag
     5041 ctctcgacgg cggacatggg atcatctgtc caccaccgca cgcgtcccag gctcagcgta
     5101 gcgcacctgc ccagcgagaa cccgccgtcc ggtgcataac cgccgcagga gtgctccatg
     5161 ccgaccgcct caaccgcgaa ttgccagccc ggcgggacca gaacatgtgt atagttaccc
     5221 ggctcgaggt cgttagtgct gaccgcgaat tccaatatcg gttccaccgc ggcttccaca
     5281 acgtacgcgt acgcccgcgg cccggcgccg ccgcaggacc ggccttggca taccaccgtc
     5341 tggcccgccc gtgcgcccat gcccatgccc gccagtacga tggcaaagaa ttgcagccag
     5401 cgcatcgtct gctcctttca ctccctcggg cgaccacccg caggttaccc agcgccgctc
     5461 cagtatacga ctaagccggg cctgaccgaa cggattcttt cacgaaaacg aacgcgccag
     5521 gcatcagaat gccagccggg cggcaatcgg catccgccgc ccgaacccga aagcccgcga
     5581 ggtgacctta agccccggcg cggcctgctg acgcttgtac tcgttcaaat gtattttgcg
     5641 gaccacctcg gtgaccatgg ccgaatcgaa gcccgccgcc acgatctgct ctatgctctc
     5701 cagtcgctcc tcatagcgcg ccaggatttc atccagcacc tcgtacggcg gcagcgaatc
     5761 ctggtccgtc tggtttggcc gcaattcggc cgagggcggc ttggtcagcg tgctccgcgg
     5821 gacaacctcg cggccggcgc ggcggttgat ctcatgcgcc agctcgtaca ccatcgtctt
     5881 gggcacgtcg ctgatcagcg ccagtccgcc gcacatatcc ccatatagcg tgcagtaacc
     5941 gaccgccagc tcgctcttgt taccggtagt cagcagcagc cggccgaatt tgttggagag
     6001 cgccatcaag cttacgccgc gtatgcgggc ctggatgttt tcctccgtca cgtccggcgg
     6061 ccgtccggcg aagtgcggcc gcagctcacg ctcgaaggcc gcgtgcagcg gctcaatcgg
     6121 aatgatggag aaatcgatac ccagattctc cgcgagggcc ttggcatcgg cgacgctgtg
     6181 ctcgctgctg taccggctgg gcatcgccac gcccagcacg ttgggcggtc cgagcgcctc
     6241 ggccgccagg caggccacga cggccgagtc caagccgcct gaaagtccga ccacgacgcc
     6301 ggtgaaacgg cacttccgca tgtagtcgcg cagccccata accagggcgg cgcgcaagtt
     6361 tgcgacgccg gtcggcatcg cttccaggcg cgcggagtcg aggtcgtcca ggtcaatcag
     6421 cagcaagtct tcttcgaacg ccttggcctg cgccaccaac cgcccggtcg cgtccacgac
     6481 gctgctcagg ccgtcgaaca gcagctcgtc gtttccgccc acctggttgc agaacagcag
     6541 gggagtcccg taccgccgcg cgtgcccggc catcagctcg aggcgtgatt cgtgcttgcc
     6601 caggtaatac ggcgaggccg aaatatttat cagcaggtca gcgccggcgg ccgccaggtc
     6661 gcaaggcgga tcaatgtcat acagcgggcg cgccagcagt tgctgcgtcg accacatgtc
     6721 ttcgcagatc atcactccca cccggcgccc ccggaagtcg atcaccggct gcactccgcc
     6781 gggctcgaag tagcgcgcct cgtcaaagat gtcatacgtg ggcagcaacc gcttgcgcga
     6841 acgcgctacg atctggccgt ccgcgatgaa ggcggccgag ttgaacaccg ggcggccgat
     6901 gccgccttcg ttgggttccg cgaatccgac gatgacgccg gcctgtaatc cggcggtggc
     6961 ggcgacgatg cgttgcagcg cggccaggtt ttgctcaatg acctcgcggc gcagcagcag
     7021 gtccttggcg ggatagccga ggatgctctg ctccgggagg acgactagtt cggcgccggc
     7081 gctggtggct cgggcgaagc cgtccagaat gcgcgcggtg tttccctcta cgtcgccgat
     7141 ggtgggattg agttgaaccg ccgcgatttt cactcaagta cctcatggtc gccgcaagcg
     7201 gctaccgtcg gagtttacca gtttgatccg gcgtacccgc ggcagcaagg ccgcctggcg
     7261 ggccggtttg tggcaactca catgttctta ggccgcaacg tcttacgcct cacgcggcgc
     7321 aggtcgaatt aacggggcgt cccggctccg cagcgggcgt tatcggtcgc cggccgttca
     7381 gaaaaatgtt ttccaaaggc cattattatg ttagactgcc agtctgagcg aaggtacttc
     7441 tccgggtgaa cgggccggcc gcctcggacg gcggccagcg tcaagcgaag gcgttgtgcg
     7501 cccaggcagg gcgattggag caaacccaaa cattagtcaa caggagaaaa ggtatgagga
     7561 gcaatttgtt cagggccgcc atgagcgcgc tggcggtggc agcttgcctg tcgccggccg
     7621 cgttcgcggt ggagcgggtc gtgctatgtg aagaattcac ggccactaac tgaggctact
     7681 gcacatacgc cggtcaggcg ttaggcgcag cgctggatag ttatcccacc actttcgcag
     7741 cgaatcagat tcacctgggg gattcatacg cggtcagttg gggttcgacg cggggctcgt
     7801 tctacagcat cagcggcact ccgacggcct ggttcgacgg catcctccag cgggtgggcg
     7861 gcacatccgg catgacttat gcctccgagt acaacacccg ccacaacatt ccgagcgaca
     7921 ttacgatgct ggttggcgcc tctgaagtcg atccgcccac cagcccgccg acctacgagg
     7981 tccagatcag cctcacgctg gacccgacgg cctccgccag aacggtgcgc gtgtatgctg
     8041 tgcaggtgct cgactattat ccaacctcgc cttcatactc ccgaaattgc atgcgcactt
     8101 ggacctcgac tgaggacgtc gcgctggttc cgggccaaac cgtgctgatc accaagatgc
     8161 tgcaaattga tacggtcagt gcggccaacc ccgacaacat gacgctgatc gcctgggcgc
     8221 aggtgccgaa ctccagcgca ccggccgagg tgtaccaggc ggcgatgatt aactatccgt
     8281 tcgaggcgtt gtacccgatc aagatcacgc tgccggaggg cgtgcctgag ttcattccgc
     8341 cagatgagga taccgccctg acggtgcgca tccagaacgg cggcgaggac ttgctgccgg
     8401 attcgcccct gatgcactac cgctacgacg acggcgcttt ccagaccgcg ccgctggttc
     8461 cgtttggcgg cgagtacttc acggccacgc tgcccgcccc gggttgcggc gccgagccgg
     8521 agttctactt cagcgcccag ggcgacggcg gctcgacggt catctacccg gagaacgcgc
     8581 cggctgaggt ggtga
//
LOCUS       DAOSYS010000158         8490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7119_length_8490_cov_5.586485, whole genome shotgun sequence.
ACCESSION   DAOSYS010000158 DAOSYS010000000
VERSION     DAOSYS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_8490_cov_5.586485"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLU99_10365"
     CDS             <1..315
                     /locus_tag="PLU99_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOO17500.1"
                     /translation="KEALRLDGWLREAAPSTELRRWFSHDPAKWAEFRRRYFAELDRN
                     PDAWRPLAEAARRRGVTLMYSSHDTEHNNAVALKEYLEAHLRARPARGARSAAVRGSA
                     RK"
     gene            381..1583
                     /locus_tag="PLU99_10370"
     CDS             381..1583
                     /locus_tag="PLU99_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO17501.1"
                     /translation="MLERSYKYIVVGGGLAGASAVDAIRESDPNGPLLLIGAENHLPY
                     DRPPLSKSLWFGRKQPRDVILHDRPWYLNRGIELVQATRVVALDAGQKRIIDERGHVY
                     RFDKLLLATGGTPRRLTIPGGELPDICYYRGMDDYLRIHRLAAQAQSAAVVGGGFIGS
                     ELAAALCHIGLGVTMIFPGQYLCQRVFPESLGRALNDKYRQRGIEIWHDDTPIAIARD
                     NSGFLLRTRAGRELRCDLVIVGIGIDPEVELARQAHLQITNGIEVNDRLQTSHPDIYA
                     AGDNARFWYEALHRRTRVEHWDNALSQGRTAGRNMAGAREQYTHMPYFFSDLFEFGYE
                     AVGEIDSTLTTVAGWKKEHDTGTIYYLRDESVRGVMLCNIWDKVDWARGLIRRGEVIA
                     PAQLGTVV"
     gene            1610..3133
                     /gene="zwf"
                     /locus_tag="PLU99_10375"
     CDS             1610..3133
                     /gene="zwf"
                     /locus_tag="PLU99_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOO17502.1"
                     /translation="MGQSDRTPPTVLVIFGAGGDLAWRKLVPALYHLFVDKWLPEQFQ
                     ILGIDRKPLDDRQFRERLRSGAMHFARYAPELPNWGRFEQHARYVAGDIHTPAPYARL
                     AGELPAQDQAWGAPAQHIFYLAVPPHLIETVVSHLGQAGLVADRGRTRVVIEKPFGHD
                     LDSAARLNRLLLESLAESQIYRIDHYLGKETVQNILALRFANSLFEPIWNRRYIDHVQ
                     ITVAEKLGVEHRGRYYERAGALRDMVQNHLMRVLANIAMEPPVTFEADEVRNKRLDVL
                     CAIRPIARADLSKYAARGQYGPGQIDGRPVPGYRQEPDVAPDSATETYVALQLFVDNW
                     RWQDVPFFVRTGKRLPVRLSQACIQFKPVPHRSFPPSAVQAWEPNRLIVYITPEEGVR
                     LHFLAKRPGLEMHLDQVWMRFSYREFFQTPPPDAYETLLLDVMRGDASLFMRADQTEM
                     SWTVVQPILEGWVDQRPTDFPNYAAGQWGPSIADELLAREGRSWFNFTSLDAPDRGY"
     gene            complement(3166..4212)
                     /locus_tag="PLU99_10380"
     CDS             complement(3166..4212)
                     /locus_tag="PLU99_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOO17503.1"
                     /translation="MKSDIVDLRSDTVTRPSPGMRAAIAAAEVGDDVLGDDPTVLRLQ
                     ERVARLLGKEAALFVPSGSMANLVAIRGWTQPGDEIIADVTVHSYNYESAGPAAIAGC
                     SFRLLNGPRGIYSAADVEAAIRPPESHFPRTRLVIIENTTNRGGGSIWPVETVAEVGA
                     TAHRHGLILHIDGARLLNACVARKLDPVEYTRHADSVSICFSKGLGAPIGSIVAGPKD
                     FIARAHRVRKMLGGGMRQVGILAAAAEYALDHNVERLAEDHAHARRLAEAISVLPGIR
                     LNPRDVETNIVLFEIEDSLGSAAEFVARMRDEGVWMFATGPRQVRALTHLDVSAAQIE
                     RAIAVFQQVCRRAC"
     gene            complement(4202..5128)
                     /locus_tag="PLU99_10385"
     CDS             complement(4202..5128)
                     /locus_tag="PLU99_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17504.1"
                     /translation="MYRLVSMFLVAFCALQTAEAQQTIQWRTDVPAAVATAKNKSLPL
                     MFYVLGKNEDRDNNLDNAQKKAFTDPKVLQLSRRFVTVRLSRSAHRDVLQELRLPATM
                     TQEIAFATPDGKVIDRISPSGVADPKTLADKMRMVFDSYRRDLFTREIQPELDKPDAD
                     PQRLLAGLGAIRDLNIVSADAALIRLTEREKLDNKVRFETYEVLAALSTKPAVEKLFE
                     LAKAEKPRAAEALGKITPAGAEILADKLVEDDGTVQIVAYDAICKACGIRERRATKWW
                     ERAENRLKLEELEKTKQAAKEAAERWRATYEE"
     gene            5334..5705
                     /locus_tag="PLU99_10390"
     CDS             5334..5705
                     /locus_tag="PLU99_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO17505.1"
                     /translation="MARRSFLLLLATFALLGGALSCRKPTSEVRAELVSGTPTQQARA
                     AVRLAGRQDVESVPDLIRLLDDQDPAVRMYAIMALQDLTGQTYGYKFYASEQERAAAV
                     QRWREALRAGEIPAVVRQEGV"
     gene            5788..6285
                     /locus_tag="PLU99_10395"
     CDS             5788..6285
                     /locus_tag="PLU99_10395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17506.1"
                     /translation="MSALSQQKDLLELELPSDPRFLAEVRRPLRDWSLQHRWTAQQAA
                     KIALAVDEAITNIIRHAYKCDPNNRILLTVRAIRKPSEGVEIRLRDFGQFCNPEQIQG
                     RALEDIRPGGLGVHIIRAMMDFVEYAPAPGGGTLLIMRKFRASKAAKPEVAKNAEPRK
                     RSANQ"
     gene            6251..6601
                     /locus_tag="PLU99_10400"
     CDS             6251..6601
                     /locus_tag="PLU99_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO17507.1"
                     /translation="MQNPAKGLRINSAVRESGPVVRVSGEVDLRTSPQMREVLLRVAH
                     KNPKRLIVDLSDVSYMDSSGVGTMVEIKRLVERGGGRLVLAGLQPRVRSVFEVTQLDR
                     FFDIAKDINDATHL"
     gene            6598..7455
                     /locus_tag="PLU99_10405"
     CDS             6598..7455
                     /locus_tag="PLU99_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17508.1"
                     /translation="MSDSPESAGSAFWFRGPAAVGAGLRAALDRVGRGFVYVGGITRL
                     LGTVIQRTWYAVRGRGTRIHASSLTAQMVRVGVRAIPLTILVQIFIGIILALNMAPTL
                     QAYGQIQRTADIIAIAIFRELGPLITAVLLSGFAGASIAAELGAMVEGEEIKALRAHA
                     LDPIRFLVIPRIVATAVMMLGLTVLADVMGIIGGLLVSVTTLNLTVAGYLTSTQLALT
                     LHDYFTGLFKGGVFGGIIAMIACYEGLNTSGGAEGVGRATTTTVVKSIVSLIGADALF
                     TAIFYAVGW"
     gene            7475..8428
                     /locus_tag="PLU99_10410"
     CDS             7475..8428
                     /locus_tag="PLU99_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17509.1"
                     /translation="MPIVIGIDEAGYGPQLGPLVLGATVWQIRPDVLEQDHWQCLKDV
                     VCRNPGRREWRLPVNDSKQIHDTNCIAPLERTVLAFALNAGLTTGRLDQFLCGLCGAA
                     AEFGPPWYRNLAQPLPIDRVRSAHTAIAARLGRAMDACGLRCCGLLAEVVPEDQFNRR
                     MAQTHNKAELLLEGVLRLMHRATAACGEQDVHIHVDRLGGRDDYGPMLLRAFPQRYLH
                     VHEVSETCSRYRLATQRSDWLIDFTVDGDQVHMSVALGSMLAKYIREALMRQFNAYWR
                     SLLPDLRPTAGYYGDARRFLSDIQPVVARTGLKWDDFVRVA"
BASE COUNT         1504 a         2751 c         2652 g         1583 t
ORIGIN      
        1 aaggaagcgc tgcgcttgga cggctggttg cgtgaagcgg cgcccagcac cgaattacgc
       61 cgctggttca gccacgatcc ggccaagtgg gccgagttcc ggcgacggta cttcgctgag
      121 ctggatcgca acccggatgc ctggcgaccg ttggccgagg ccgctcggcg gcgcggcgtg
      181 acgctcatgt acagttcgca tgacactgaa cacaacaatg cggtcgcgct taaagagtac
      241 ttggaagcgc atttgcgtgc gcgaccggcc cgcggggcgc gtagcgccgc agtgcgcgga
      301 tcggcgcgga aataggttac ggccgcagcg ttgccgccta tgtcaactgt ggaacagcgc
      361 tcagcttgga aggaacaaac atgttggaac gtagctataa gtacatagtt gtgggcggcg
      421 gcctggcagg agcttcggcc gtggatgcta tccgcgaaag cgatcccaat ggcccgctcc
      481 tgctgatcgg ggcggaaaac cacttgccgt acgaccggcc gcccctctcg aagtcgctgt
      541 ggtttggcag gaagcagccg cgggacgtca tcctgcacga ccggccctgg tacctcaatc
      601 gcggcatcga gctagtgcag gccacgcgcg tggtggcgct ggatgcggga cagaaacgca
      661 tcatagacga gcgcggtcat gtctatcgtt tcgataagtt gctgttggcg accgggggca
      721 cgccgcggcg cctgactatt cccggcggcg aattgccgga tatctgttat taccgcggca
      781 tggacgatta cctcaggatt caccggctgg ccgcgcaggc tcaatcggcg gccgtcgtgg
      841 gtggcggctt catcggctcc gaactggccg cagcgctctg ccacattggc ctgggcgtca
      901 ccatgatttt ccccggccaa tatctttgcc agcgcgtgtt ccccgaatca ctcggccggg
      961 cgcttaatga taagtaccgg cagcgcggca tcgaaatctg gcacgatgat acaccgatag
     1021 ccatcgcacg agacaattca ggcttcctgc tgcgcacccg cgccggccgc gaactgcgct
     1081 gtgatctggt gatcgtcggg atcggcatcg acccggaggt agagctggcc aggcaggcac
     1141 accttcagat aactaacggc atcgaagtca atgaccggct gcaaacctcg catccggaca
     1201 tttatgcggc gggcgacaat gcgcgcttct ggtatgaggc cttgcaccgc cgcacgcggg
     1261 ttgagcactg ggacaatgcg ctgagccagg ggcgcacggc cggccgcaac atggccgggg
     1321 cgcgggaaca atatacccac atgccgtatt tcttctccga tctctttgaa ttcggctatg
     1381 aggccgtggg cgaaatagat tccacgctga cgacggttgc gggttggaaa aaagagcacg
     1441 ataccggcac catctactat ctgcgcgacg agagcgtgcg gggcgttatg ctgtgcaaca
     1501 tctgggacaa ggtggactgg gcgcgcggcc tcatccgccg cggtgaagta atcgctcccg
     1561 cgcaactggg cactgtggtt tagcaacgcc actgggttgc aggtgcatta tggggcagtc
     1621 tgacaggacc ccgccgacgg tgctagtcat cttcggggcg ggcggcgacc tcgcctggcg
     1681 gaagctcgtc ccggctctgt atcacctgtt cgtggacaag tggctgcctg agcagttcca
     1741 gattttgggg attgaccgca agcctctcga tgaccggcag tttcgggagc gcctgcgtag
     1801 cggggccatg catttcgccc ggtatgctcc cgaattgccg aactggggcc gttttgagca
     1861 gcacgcccgg tacgttgccg gcgacatcca cacgcctgcc ccctacgcgc gtctggccgg
     1921 agaactgccg gcccaggacc aggcctgggg cgcgcccgcc cagcacattt tctacctggc
     1981 cgttccgccg cacctgatcg agacagttgt ctcgcatctt ggccaggccg gcctggtcgc
     2041 cgaccggggc cgcacgcgcg tggtgatcga aaagccgttt ggacacgacc tcgattcggc
     2101 cgcaaggctt aaccgcctct tgctggagtc cctggccgag tcccagattt accgcataga
     2161 ccattatctc ggtaaagaaa cggtgcagaa catcctggcg ctgcgttttg ccaacagtct
     2221 ctttgagccg atctggaacc ggcgctacat cgaccacgtg cagattacgg tggccgagaa
     2281 actgggcgtc gagcaccgtg ggcggtatta cgagcgcgcc ggcgcgctgc gcgacatggt
     2341 gcagaaccac ctgatgcggg tcctggccaa catcgccatg gaaccgcccg tgaccttcga
     2401 ggccgacgag gtgcgcaaca agcggctgga cgtgctctgc gccattcgtc cgatcgcgcg
     2461 ggccgacctg tccaaatacg ccgcgcgcgg ccagtacggc cctggccaga tcgacggccg
     2521 gccagttcca ggttatcgcc aggagcccga cgtggcgccg gactcggcca ccgaaaccta
     2581 tgtggcgcta cagctcttcg tcgacaattg gcgctggcag gacgtgcctt ttttcgttcg
     2641 caccggcaaa cggctgccgg tacgtctgtc gcaagcctgc attcagttca agcctgtccc
     2701 gcatcgctcg tttcctccgt ccgcggttca ggcttgggaa cccaatcgcc tgatcgtgta
     2761 catcacgcct gaggagggcg tacggctgca ttttctcgcc aaacggccgg ggctcgaaat
     2821 gcatcttgat caggtttgga tgcggttctc gtaccgcgag ttctttcaga cgccgccgcc
     2881 ggacgcttac gaaacgctgc tgctcgacgt aatgcgcggc gacgcctctc tgttcatgcg
     2941 cgctgaccag acggagatgt catggactgt cgtgcagccg attttggagg gctgggttga
     3001 ccaacgcccc accgacttcc ccaactatgc cgccggccaa tggggcccca gcatcgccga
     3061 cgagctgctg gcgcgcgagg gccggagctg gttcaatttt accagcctcg atgctcccga
     3121 tcgcggctac taacccttta agttcgtgct ggcgccaatc gaggatcaac aagcccgccg
     3181 gcacacctgc tggaagacgg cgatggcccg ctcgatctgc gcggccgaca cgtccaggtg
     3241 cgtcagcgcc cgcacttgcc gcggcccggt ggcgaacatc cacacgcctt catcgcgcat
     3301 gcgcgctacg aactcagccg ccgagcccag cgaatcctca atttcgaaca ggacgatgtt
     3361 ggtctctacg tcgcgtggat tgagccgaat tcccggcaga acgctgatcg cctcggccag
     3421 ccgccgcgcg tgggcatggt cttccgccag ccgctcaacg ttgtgatcga gagcatactc
     3481 tgccgccgcc gccagaattc caacctgccg catgcccccg cccaacatct tgcgtacgcg
     3541 atgcgcgcgg gcgatgaagt ccttcggccc ggccacaatc gaaccgatcg gcgcgccgag
     3601 ccccttcgaa aagcagatcg acaccgaatc agcgtgccgc gtgtactcga ccgggtccag
     3661 cttgcgggcc acacacgcgt tcaacaggcg cgcaccgtca atgtgcagga ttagcccgtg
     3721 gcgatgcgcc gttgctccca cttccgccac cgtctcaacc ggccagatcg agccgccacc
     3781 ccggttggtg gtgttttcaa taatgaccag ccgcgtgcgc ggaaagtgtg attccggcgg
     3841 gcgaatagcc gcttcaacgt ccgcagcgct gtaaattccg cgcggcccgt tcagtaggcg
     3901 gaagctgcac ccggcgatcg ccgccggtcc ggccgactcg tagttgtaac tgtgaacggt
     3961 aacgtcagcg atgatctcgt cgcccggctg cgtccagccg cggatggcca ccaggttcgc
     4021 catgctgccg gagggcacga acagggccgc ttccttaccc agcagccgtg ccacgcgctc
     4081 ctgcagccgc aggacggtcg ggtcgtcgcc caggacgtcg tcgccgacct cggccgcggc
     4141 gatcgcggcc cgcatgcctg gcgaaggccg cgtaacggta tcgctacgca gatcgacgat
     4201 gtcactcttc ataggtcgcc cgccaacgct cggccgcctc tttcgcagcc tgcttcgttt
     4261 tttcgagctc ttccagcttg aggcggttct cggcgcgctc ccaccatttg gtggctctgc
     4321 gctcccggat cccgcaggcc ttgcaaatcg cgtcgtaggc cacgatttgc accgtgccgt
     4381 cgtcctctac cagcttgtcc gccagtatct cggccccggc cggcgttatt ttcccgagag
     4441 cctcggccgc acgcggcttt tccgctttcg ccagctcgaa cagcttttcc acggcgggct
     4501 tagtggacag cgcggccagc acctcgtacg tttcgaagcg caccttgttg tccaatttct
     4561 cacgctccgt caggcggatc agcgccgcat cggccgacac gatgttgaga tcgcgtatgg
     4621 cgcccaggcc cgcgagcagc cgttgcgggt cggcgtccgg cttatcgagc tccggctgaa
     4681 tttcgcgggt gaagaggtcg cggcgatagg aatcaaagac catccgcatt ttgtcggcca
     4741 acgttttggg atcggccacg ccgctgggcg aaatccggtc gatgaccttg ccgtcgggcg
     4801 tggcgaatgc gatttcctgc gtcatggtgg caggcaaccg cagttcctgg agcacgtcgc
     4861 ggtgggcgct gcgggagagg cgaaccgtaa caaagcgccg cgaaagctgc agcaccttgg
     4921 ggtcggtgaa agctttcttc tgggcattgt cgaggttgtt gtcccgatcc tcattcttcc
     4981 ccaaaacgta gaacatgagc ggcaacgact tgttcttggc cgtagcgacg gcggcgggga
     5041 catccgtgcg ccactggata gtctgctgcg cctcggccgt ttgcagcgca cagaacgcga
     5101 cgaggaacat cgaaaccaaa cggtacatgc caaaacctcc ttttcggcgg ccccaggctg
     5161 tcgccgcaac gataaccggc tcaaaggcgt cattctaaga gcggagccga accgtgtcgc
     5221 cccggatttc tgcgcgggga cgattgaggg gcggtccgtc ggcgaggcgc aggccgtgcg
     5281 cttaattgtc tgctatttcc gacccgcgcg cgctgacaac ctataatttc agcatggcgc
     5341 ggcgctcttt cctgctgctg ctagccacgt ttgcgctgct cggcggcgcc ctatcctgcc
     5401 gtaagcccac ttccgaggtc cgggcggagc tcgtttccgg aacgccgacg cagcaggccc
     5461 gggcggccgt gcggctggcg ggtcgtcaag acgtggaatc cgtaccggat ctgatcaggt
     5521 tgcttgacga ccaagacccg gccgtgcgaa tgtacgccat aatggcatta caggacctga
     5581 ccggccagac ttacgggtac aaattctacg cctcggagca ggagcgcgcg gcagcggttc
     5641 agcgctggcg agaggccctg cgggcgggtg aaattcccgc cgtcgtcaga caagagggcg
     5701 tttagaagcg ctcccaacga ccgtcctcgg ttgatgcgcg gcattggggc cgtaatccca
     5761 acgcgaccgt aaccgattct ctatatcatg agtgccctat ctcagcagaa agacctgctg
     5821 gagctggaac tgccgagcga tccgcggttc ctcgcagagg ttcgcagacc gctccgcgac
     5881 tggtcgttgc agcaccgctg gaccgcccaa caggcagcca agatcgcgct ggccgtggat
     5941 gaagcgatca ccaacattat ccgccacgcc tacaaatgcg accccaacaa tcgcattctt
     6001 ctgaccgtcc gggccattcg aaaaccgagc gagggagtcg aaatccggct gcgggacttc
     6061 ggtcagtttt gcaaccccga gcaaattcag ggtcgggcgc tcgaagatat ccggccaggc
     6121 ggcttgggcg tgcatatcat ccgcgcgatg atggatttcg tagaatacgc gcccgcgccc
     6181 ggcggcggta ccctgctgat aatgcgaaaa ttcagagctt caaaggcggc caagcccgag
     6241 gtggcgaaaa atgcagaacc ccgcaaaagg tctgcgaatc aatagcgcgg tgcgtgagag
     6301 cggacccgtc gtgcgcgttt cgggcgaggt ggatttgcgc accagccccc aaatgcgcga
     6361 agtccttcta cgagtggccc acaaaaatcc caagcggctg atcgtagacc tctcggatgt
     6421 cagctatatg gactcctccg gcgtgggcac gatggtcgaa atcaagcgcc tggtcgaacg
     6481 gggcggcggc cgcctcgtgc tggcgggtct ccagccgcgc gttcgcagcg ttttcgaggt
     6541 tacccagctc gaccgcttct ttgacattgc caaggatatc aacgacgcta cgcacttgtg
     6601 agcgacagcc ccgaaagcgc cggtagcgca ttctggtttc gcggccccgc ggccgtcggc
     6661 gccggcctgc gcgccgcgct ggatcgcgtg gggcgcggat tcgtgtacgt cggcggcatc
     6721 acccgcctgc tcggaacggt tattcagcga acctggtacg ccgtgcgcgg ccgtggcaca
     6781 cgaatccacg ccagctcgct gacagcccaa atggtgcgcg tcggcgtccg cgccattccg
     6841 ctgaccatcc tggtgcaaat cttcatcggc atcatcctgg cgctgaacat ggcgccaacg
     6901 ttacaggcat acggccaaat ccagcgtaca gccgacatca tcgcgatcgc cattttccgc
     6961 gaactggggc cgctcatcac cgcggttctg ctgagcggtt tcgccggcgc atctatcgcc
     7021 gccgagctcg gagccatggt ggagggcgag gagattaagg ccctgcgcgc ccatgcgctc
     7081 gacccgatcc ggtttctggt cattccgcgc atcgtggcga cggcagtcat gatgctcgga
     7141 cttaccgtgc tggccgacgt catgggcatc atcggcgggc tactggtttc cgtgaccacg
     7201 ctgaacctga ccgtcgccgg ctacctgact tcgacgcagc tcgccttgac gcttcacgac
     7261 tacttcacgg gcctgttcaa gggcggcgtc ttcggcggca tcatcgccat gatcgcctgc
     7321 tatgagggct tgaacaccag cggtggcgcc gaaggcgtcg gacgggcgac caccaccacc
     7381 gtcgtaaaga gcatcgtatc gctgatcggg gccgacgcgc tcttcaccgc catcttctat
     7441 gcagtgggtt ggtagcggcg cataatcttg acgtatgccg atcgtgatcg gaattgatga
     7501 agccggctac gggccgcaac tgggaccgct cgtgctcggg gcgacggtgt ggcaaatccg
     7561 ccccgacgtg ctcgagcagg accactggca atgccttaag gacgtggtct gccgcaaccc
     7621 cggccggcgc gagtggcgcc tgccggtaaa cgactcaaag cagatccacg acaccaactg
     7681 catcgcaccc ctcgagcgca ccgtgctggc cttcgcactc aacgccgggc tgaccaccgg
     7741 ccggctcgat cagttcctgt gcggcttatg cggggcggcg gctgagttcg gtccgccttg
     7801 gtatcgaaat ctcgcccaac cgctgccgat cgaccgggtg cgttccgccc acaccgccat
     7861 cgccgctcgc ctgggcaggg ccatggacgc ctgcggcctg cggtgctgcg gcctgctggc
     7921 cgaagtcgtt cctgaggatc agttcaatcg tcgcatggcc cagacgcaca acaaggccga
     7981 gttgctgctc gaaggcgtcc tgcggctgat gcaccgcgcg accgcggcct gcggcgagca
     8041 ggacgtgcac atccacgtcg accgcctggg cgggcgcgac gactacggtc ccatgctgtt
     8101 gcgcgccttt ccgcagcgct atctgcacgt acacgaggtt tctgagacgt gcagccggta
     8161 ccggttggcg acgcagcgca gcgattggct gatcgatttc accgtggacg gcgatcaggt
     8221 gcatatgtcc gtggccctcg gcagcatgct cgccaagtac atccgcgaag ccctcatgcg
     8281 ccagttcaac gcctactggc gcagcctgct gccggattta cgcccgaccg ccggctacta
     8341 cggcgacgcg cgacgcttcc tctccgatat ccagccggtg gttgcccgga cggggttgaa
     8401 gtgggacgac ttcgttcgcg tggcgtagtt gaggttcgag ccgcgagttt cgagtgccgc
     8461 gcggaaccct ggcaagagcg ttagaatgcc
//
LOCUS       DAOSYS010000159         8408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7209_length_8408_cov_4.780199, whole genome shotgun sequence.
ACCESSION   DAOSYS010000159 DAOSYS010000000
VERSION     DAOSYS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8408
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_8408_cov_4.780199"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..908)
                     /locus_tag="PLU99_10415"
     CDS             complement(177..908)
                     /locus_tag="PLU99_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17510.1"
                     /translation="MPYPSARAPCPSAKRGHALAAEPIRRPFASRHLTFIAAAALSAL
                     VNAACAPNSGSLFKLNLGSPFKLSSDSLFGLNPVSGITLVSYKDPYFPEPIDVPLNEC
                     TYQKDPRGDLQIVARDAAAALDTAGPLERLLHIHVYWQPYPGKTFSNSSTTTATIRYI
                     VATREGTVTYTGTGFAYPAKRSGDGLEIDLESANLQLDTSTGTAPEMLGLSRLTGKLF
                     ARHDPHRTVDLMRQVDLLAAGNTLP"
     gene            complement(1039..1914)
                     /locus_tag="PLU99_10420"
     CDS             complement(1039..1914)
                     /locus_tag="PLU99_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOO17511.1"
                     /translation="MIVDCHTQIWDPSPYAGASLVRPIPETEASRHLAAVDPVDCAIV
                     LAFKSCYLQAEVPNRVVAEYVRQNPSKMVGFAGIDPNDRDWPDQLRIAQEELQLKGVT
                     ISPAMQDYHPSATQAMRLYDACTRRGLPILFEQNHRSPAAKMEFGRPMLLDEVAREFP
                     NLRIIISHLGYPWIDETLVLLSKHPNVYADVAGLHRRQWLTYNALLTAFESGVIDKLL
                     FGSDFPHRAPTECIEALYSINQMAHGTNLKAIPREQLRGIVERDALTLLGIEQPRPAN
                     KSKSGAKSGIFVNDD"
     gene            complement(2044..>2586)
                     /locus_tag="PLU99_10425"
     CDS             complement(2044..>2586)
                     /locus_tag="PLU99_10425"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOO17512.1"
                     /translation="MTEPVVTIFGSSAIDPAGPAYQTAYDLGRRIALAGWRLCNGGYG
                     GTMEAACKGACEAGGQTIGVTCKAFNRPAANRFVRQEISTPSLLARLDKLVHLGDAFL
                     VLPGGTGTLLELALVWELLNKRLIGRQTPLILLGDFWTPLIPLVLAQQPEALRPQVAP
                     TVEAAVALLAAHFEGRPGTP"
     assembly_gap    2587..2686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2895..5801
                     /locus_tag="PLU99_10430"
     CDS             2895..5801
                     /locus_tag="PLU99_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17513.1"
                     /translation="MPGWALQGRRLLEMSGKACPRLELGHGTPHGSPFRSLLGRGLVF
                     SLAMGLLLENAAFALQPATAPVAARCESAAAAKAPSADDVSELIGRLGDSSYRVREDA
                     TRDLLLLGPEIVTALETWLPQETDPEVRFRLRFVLENLRPPQNAVLVLRSDYYSSGLY
                     AGDVITHINNQRVNSRAELLRRLRPGAMLRVRRAEGPFETLLKNRSDLPVVCDYRAPA
                     GAAIASAVRLYAYGYAEQAYELLRELKGEVPESELPALLYAVIAYTAGDAQSAFDILA
                     EHPDACLPVSLVNLSSSPSALDLAGPVGAPYHLEWRWWTERLLGDAWAEPDTAIHRVL
                     IPAGRYADALQGVAKTWWDSRQQLGKTLSEGDGGNVLAVCAWMVSELDLVSEALRLIE
                     PRSILLRRTPQGARKWIRVRTDAWLPFARGDAQAALDGFYEDARAILQPPGPPPERII
                     ENPQVAAEVAFFLYQFPRDERVADMLAVVNQPDYPPLASYAHWMLYAVRPENFELARE
                     HMLAILPNTPEREAGKFALATALLEYVRQRPDPEVIAAARAGLADAPPWSDKQEKAAL
                     ITALEHLAKGAADEAGKALSAAGAQPGVPVLRSTAEYLAASAATPPRDEGLQRPLMAV
                     RAGWSGANWLLLTRARQFVWCDLASGESDAIPARSANWFPGPLNWPWIGKEESSGRLW
                     VYGLRRVIELRPDADGPAFRTNICTEDIPAFDRYVRPNFTALADLAGDVQQEGGEAGE
                     LWRDDLLAGREYFADPELPEIGFIRVLPRDARMVQVAFRGGSHLLIDTANGRCWSSRW
                     IAEQLGLATAPRFFAQAVGPLVETPSSEDGRADGAAPGETNLYLFSDAGLLRLDSAKS
                     VVRRIELPGPEPYPALVPESCPYERRDSCWVYCARLPEEGGQVYRLSVGDDRAELLGM
                     VNEALPREYYRMQSRAALRRKVNDLLARVGVPSLREFVSETEQLLAKYAEVKDP"
     gene            5798..7330
                     /gene="hutH"
                     /locus_tag="PLU99_10435"
     CDS             5798..7330
                     /gene="hutH"
                     /locus_tag="PLU99_10435"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOO17514.1"
                     /translation="MKPVLLRLGENVLTLKVLEACLGCPVQVSITPEAMARAERAREW
                     VEDLLQSGGAVYGINTGFGSLKNKRISPDQTDQLQENLIISHAVGVGPAAPPEIVRWM
                     MLFKLHMLLQGHSGVRPEVIRLMQTCLNADMLPVVPTRGSLGASGDLAPLAHLVLPLT
                     GRGEMTVPSSGQGGGRQTRPAGELLASLGVEPLRLAAKEGLALINGTQFMSAYAANIA
                     VRAARLANLADLIGCMSLEGFRGSIRPMDPRLHEVRPHPGAQTVAANVRRWMAGSEIL
                     SSHENCGRVQDPYSLRCIPQVHGAFRDALTHFSQTVEREINSVTDNPIVFEDGAISGG
                     NFHGAPLALVLDYLAIALTDLASISERRTYQLLSGEDGLPKLLMRDTGLNSGFMLPQY
                     TAAALVNECKVLATPAAVDSIPTSLGQEDHVSMGATSAFKCFEVIDRVETVLAIELVC
                     AAQALDFRAPLLPGVGPRAAHALVRQVIPHAERDRLFGQDIAAAVQLLRHSAELRAVA
                     NQ"
     gene            7485..8393
                     /locus_tag="PLU99_10440"
     CDS             7485..8393
                     /locus_tag="PLU99_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17515.1"
                     /translation="MTRSLIKTVARLVKWRAVGLATLAIVAAGVSFAQAPATEKKPGQ
                     AQKPSAAEEKLPKAEAVLDKLVEASGGEAAINRIRTRVSKGTYEQGPAKANITMYEAA
                     PNKSLQIIELPEGLKIEQGTNGEVYWTQSPMGVQIADKEEAALAARQGDIHLPATWRR
                     HYKSAETAGIETVGSSPAYKLIMTPEEGPPELWFVDQKSNLLVRWDMRAGAGMGNISI
                     YFEDHRKVDDVMVPFRQRAAGLMPLVVTLSSVEHNVDIPKERFELPEAVKEALKDAGK
                     APALPGHPPQPPKPDRPDKPEKPGNP"
BASE COUNT         1504 a         2457 c         2845 g         1502 t
ORIGIN      
        1 gtcagatcca atcggaaggg tgcgaccggt cgcaggaaaa aacctgccat ccactgattt
       61 tagacgacgc ggaacctggt cacgggtaag gctaattacc cgcaccggct agcaattgcc
      121 agggctagcg agtgccgtgg ggtatcatgg tttggctacg gtagtggtgc ggttggctag
      181 ggcagtgtgt tgccggccgc cagcaggtcg acctgccgca tgaggtcgac ggtccggtgg
      241 ggatcgtgcc gggcgaagag cttgcccgtc agacgcgaga ggcccagcat ttccggtgcg
      301 gtgcccgtgc tagtgtccaa ctgcaagttt gcggattcca agtcgatttc gagtccgtca
      361 cccgaccgct tggcgggata cgcaaagccg gttcccgtgt acgtaacggt cccctcccgc
      421 gtcgccacga tgtagcggat cgtggccgtc gtcgtgctgg agttggaaaa cgtcttgccc
      481 gggtacggct gccagtaaac atggatgtgc agcaggcgtt cgagcggacc ggccgtatcg
      541 agcgcggccg ccgcgtcgcg ggcaacgatc tgcaagtcgc cgcgggggtc cttctggtac
      601 gtacactcgt tcagcgggac atcaattggc tcggggaaat atgggtcttt gtaagacacc
      661 agggtgatgc ccgaaacggg attcagccca aaaagcgaat cggagctcag cttgaacggc
      721 gaacccaggt tcagcttaaa gagtgagccg ctgttcgggg cgcaggccgc gttgacaagc
      781 gcgctcagcg ccgcggctgc gatgaatgtc aggtggcgcg aggcgaaagg tcgccggatg
      841 ggttcggctg cgagggcatg cccgcgcttg gcgcttgggc atggcgcccg cgcgcttggg
      901 tatggcaccc gcgcctcgtc gcctatttga cgggcgggcg tggtttgacc ggccggcgcg
      961 gtttgaccgg cggacgcggt ttgacccgct ggcgcggtct gacttggcga cgctttgtct
     1021 ggttccgccc ggccctggct aatcatcatt cacgaagatc ccgcttttgg ctccgctctt
     1081 ggacttattg gccgggcggg gctgctcgat gcccagcagc gtgagcgcgt cgcgttcgac
     1141 gatgccgcgc aactgttccc gcggaatagc cttcagattc gtgccgtgag ccatctgatt
     1201 gatagagtac agagcttcaa tgcactccgt cggagcgcgg tgcggaaagt cgctgccgaa
     1261 aagcagctta tcgatcacgc cggattcgaa cgccgtcagc agcgcgttat acgtcagcca
     1321 ctgccggcga tgcaggccgg ccacgtcggc atagacgttc gggtgcttgc tgagcaggac
     1381 cagcgtttca tcgatccagg gataacccag gtggctgatg atgatccgca gattggggaa
     1441 ctcgcgcgcg acctcatcca ggagcatcgg ccggccgaat tccattttcg ccgccggact
     1501 gcgatggttc tgctcgaaga ggatgggcag accgcgccgc gtgcaagcat catacaggcg
     1561 catcgcctgg gtggccgagg ggtggtaatc ctgcatagca ggggagatcg tcacgccctt
     1621 gagctggagt tcttcctgag cgatccgcag ttggtcaggc cagtctcggt cattcggatc
     1681 aatgccggcg aaacccacca ttttggacgg gttttgtcgg acgtactcgg cgaccacccg
     1741 gtttgggact tcggcctgca aatagcagct cttaaacgcc aggacgattg cgcagtccac
     1801 gggatcgacc gctgccaaat ggcgcgatgc ttccgtttct ggaatgggcc ggaccaggct
     1861 tgcgccggcg tacggactcg gatcccagat ctgcgtatgg cagtcaacaa tcatggcggc
     1921 agcttacctg ttcaaccttc ccacaccgcc tcaatgcatc ctcgaccgcg ctcccatcaa
     1981 ggaacgggaa ggatggtaat gcagcacccc gagggcggtc aagtcgccga tgacgggcga
     2041 gcgtcagggg gtgccgggtc taccttcgaa gtgcgccgcc agcagcgcca ccgccgcttc
     2101 caccgtcggc gctacctgcg gcctgagagc ttcgggttgc tgggccagga cgagcgggat
     2161 gaggggcgtc cagaaatcgc cgagcaggat tagcggcgtt tgccgtccga tcaggcgttt
     2221 attcagcagc tcccagacca gcgcgagttc cagcaaggtg ccggtaccgc cgggcaaaac
     2281 caggaatgcg tcgccgagat gcacgagctt gtccaaacgg gccaggagcg agggcgttga
     2341 aatttcctgg cgtacaaagc gatttgcggc ggggcggttg aacgccttgc aggttacgcc
     2401 gatcgtctgc ccgccggcct cgcaggctcc tttgcaggcc gcctccatgg tcccgccgta
     2461 gccgccgttg cacagtctcc agccggccag ggcaattctg cggccgaggt cgtaggcggt
     2521 ctggtaggcc gggccggctg gatcaatggc agagctgccg aagatcgtta cgactggttc
     2581 ggtcatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggcc ggctggatca
     2701 atggcagagc tgccgaagat cgttacgact ggttcggtca tccttggcct cgctgctggg
     2761 ttccggccca tgttaacgat agtggccggc caactcgaat gttgaaaggg gaacctcgaa
     2821 aacaggccgg atgtgtaccg cgggcgcgtt gccccgtaga ctcatgccat gagctgtgtg
     2881 ccggagagca ggtgatgccg ggctgggctt tgcagggacg acggctgctt gagatgtccg
     2941 gcaaggcatg cccgcgctta gagcttgggc atggcacccc gcatggcagc ccgtttcgta
     3001 gcctgcttgg gcggggcttg gtcttctccc tggcgatggg cctgttgctg gaaaacgctg
     3061 cgttcgcgct gcagccggcg acggcgccgg ttgcggccag atgcgaaagt gcggcggccg
     3121 caaaggcgcc gtcggcggat gacgtgagtg agctgatcgg gcggctgggt gactcgagct
     3181 accgggtgcg cgaggacgcc acgcgggacc tgttgttgct cggcccggaa atcgtcaccg
     3241 ccctggaaac ctggctgccg caggaaaccg atcccgaggt gcgcttccga ctgcggttcg
     3301 tgttggaaaa cttgcggccg ccgcagaacg cggtcttggt gctacggtcc gactactaca
     3361 gcagcggttt atatgctggg gacgtgatca cgcacatcaa caaccagcgc gtaaacagcc
     3421 gggcggagtt gctgcgccgg ctgcgtccgg gcgcgatgct gcgcgtgcgc cgcgcggagg
     3481 gtccgttcga gacgttgctg aagaaccgca gcgacctgcc ggtggtgtgc gattaccgtg
     3541 cgccggcggg tgcagcgatc gccagcgcgg tgcggctata cgcttatggc tatgccgagc
     3601 aagcatacga gttattgcgg gaattaaagg gcgaggtccc ggagtccgaa ctgccggcgt
     3661 tgctatatgc agtaatcgct tacacggcgg gtgacgcgca gagcgcgttc gatattctgg
     3721 cggaacaccc ggacgcctgc ctgccggtgt cgctggtaaa tttgtccagc agcccttcgg
     3781 cgctggattt ggccggcccg gtgggagcgc cgtaccacct cgaatggcgc tggtggactg
     3841 aacgcttgct gggcgacgcc tgggccgagc ccgacacggc gatccatcgc gtgctgattc
     3901 ccgccggccg gtatgcggat gcgttgcagg gggtggcgaa gacctggtgg gattcgcgcc
     3961 agcaattggg gaaaacgcta agtgaaggcg atggcgggaa cgtactggcg gtgtgcgcct
     4021 ggatggtttc cgaattggac ctggtcagcg aagcgctgcg gctgatcgaa ccgcgcagca
     4081 ttctgctgcg ccgcacgccg caaggggcgc gcaagtggat acgcgtgcgg acggacgcct
     4141 ggctgccatt tgcgcgcggc gacgcacagg ccgcgttgga cggtttttat gaggacgcgc
     4201 gcgcgattct ccagccgccc ggtccgccgc cggagcgcat cattgagaac ccgcaagtcg
     4261 cagccgaagt agccttcttt ctctaccagt ttccgcggga tgagcgggtt gcggacatgc
     4321 tggcggtcgt gaaccagcct gactacccgc ccttggcatc ctacgcgcac tggatgctct
     4381 atgccgtacg gccggaaaac ttcgagctgg cgcgcgaaca catgctggcc atcttgccta
     4441 acacgccgga acgcgaggcg ggcaagttcg cgctggcgac ggcgctgctg gaatacgttc
     4501 gccagcgacc cgacccggaa gtcatcgctg ccgcgcgggc aggtctggcc gacgccccgc
     4561 cctggtccga caagcaagag aaggccgcct tgattacggc cctggagcat ctcgcgaagg
     4621 gcgcggcgga tgaggccggc aaggcgttga gcgcggccgg cgcccagccg ggcgtgccgg
     4681 tgctgcgcag cacggccgag tacctggccg ccagcgccgc cactccgccg cgggatgagg
     4741 ggctgcagcg gccgctcatg gcggtacgcg cggggtggag tggcgcaaat tggctgctgc
     4801 tgacgcgggc gcggcaattc gtgtggtgcg atctggccag cggtgagtcg gacgcgattc
     4861 ccgctcgcag cgcgaattgg ttccccggcc cgctgaactg gccatggatt ggaaaggaag
     4921 aatcatccgg gcgtctgtgg gtatacgggc tgcggcgagt catcgagctt cgcccggatg
     4981 cagatggtcc ggcgttccgg acgaacattt gcaccgagga cataccggcg ttcgatcgct
     5041 acgtgcggcc gaacttcacc gcgctggccg atttggcggg tgacgtgcaa caagaggggg
     5101 gcgaggcggg tgagttgtgg cgcgacgacc tgctggcagg gcgggagtat ttcgcggatc
     5161 cggagttgcc ggagatcggg ttcatccgcg tgctgccgcg ggacgcgcgc atggtccagg
     5221 tcgcgtttcg cggcgggtcg cacctgttga tcgatacggc aaacgggcgg tgctggagtt
     5281 cgcgctggat cgcggagcag cttgggttag cgaccgctcc gcgttttttc gcgcaggcgg
     5341 tcggcccgct ggtcgaaacg cccagcagcg aggatggccg cgcagacggc gccgcgcccg
     5401 gcgaaacgaa cctgtattta ttcagcgacg ccggactatt gcggctggat tccgcaaaaa
     5461 gcgttgtgcg ccgcatcgag cttcccgggc cggaaccgta tccggcgctg gtccccgaat
     5521 cgtgccccta cgagcggcgc gattcgtgct gggtgtactg cgctcggctg ccagaggagg
     5581 gcgggcaggt ttaccgccta agcgtgggcg atgaccgagc ggagttgctg ggcatggtaa
     5641 acgaggccct gccacgcgaa tattaccgga tgcagtcgcg cgctgcgttg cggcgcaagg
     5701 tgaatgattt gctggcgcga gttggcgtgc cttctctgcg cgaattcgtg agcgaaaccg
     5761 aacaactttt ggcaaagtac gcggaggtca aagatccgtg aagcccgttc tgctgcgctt
     5821 gggtgaaaac gtattgacgc tgaaagtgct ggaggcgtgc ttggggtgtc cggtgcaagt
     5881 ctcaatcacg cccgaggcaa tggcgcgcgc tgagcgggcg cgcgaatggg tggaggacct
     5941 cctgcaaagc gggggggcgg tttacggcat caacaccggt tttggatcgc tgaagaacaa
     6001 gcgcatctcg ccggaccaaa ccgatcagct ccaggagaat ctcatcatct cgcacgcggt
     6061 cggcgtgggg ccggccgcgc cgccggagat cgtgcgctgg atgatgctct tcaagctcca
     6121 catgctgctg cagggacaca gcggcgtgcg ccctgaagtg atccgcctga tgcagacctg
     6181 cctgaacgcc gacatgttgc cggtagttcc cacgcgcggc tcgctcgggg ccagcggaga
     6241 tttggcgccc ctggcgcacc tggtgctgcc gctcaccggg cgcggcgaaa tgaccgttcc
     6301 cagctcagga cagggcggcg gacgccagac ccgccccgcc ggcgaactgc tcgcgtcgct
     6361 gggcgtggag ccgttgcggc tggcagccaa ggaaggcttg gccctgatca acggcacgca
     6421 attcatgtcg gcctatgccg ccaatatcgc cgtccgggcc gctcgcctgg cgaacttggc
     6481 ggacttgatc ggctgcatgt cgctggaagg cttccgcggc agtattcggc cgatggaccc
     6541 gcggctgcac gaagtgcggc cgcatccagg cgcgcagacg gtcgcggcaa acgtgcggcg
     6601 gtggatggcg ggcagtgaga tcctgtcctc acatgagaac tgcggccggg tgcaggatcc
     6661 gtattcgctg cgctgcattc cgcaagtaca cggggcgttt cgcgacgcac tcacgcattt
     6721 tagccagacg gtcgagcgcg aaatcaatag cgtgacggac aacccgattg tgttcgaaga
     6781 cggcgccatc agcggcggca actttcatgg cgcgccgctg gcgctcgttc tggattacct
     6841 ggccatcgca cttaccgacc tggcgagcat ctcagaacgc cgcacttacc aactactcag
     6901 cggcgaggac ggcttgccga agctgctgat gcgcgacacc ggactcaaca gcggcttcat
     6961 gttgccgcag tacacggccg ccgcactggt gaacgagtgc aaggtgttgg ccacgccggc
     7021 cgcggtcgat tcaatcccca cttcgctggg gcaagaagac cacgtgagca tgggcgccac
     7081 ctcggctttc aaatgttttg aagtgatcga tcgcgtcgaa accgtgctgg cgatcgagct
     7141 cgtttgtgcc gcacaggcgc ttgattttcg tgcgccgctc ttgccgggag tcggcccgcg
     7201 cgccgcgcat gccctggttc ggcaggtcat tccgcatgcc gagcgagacc ggcttttcgg
     7261 gcaggatatt gcggccgccg tgcaactcct gcgtcacagc gcagagctgc gcgcagtggc
     7321 taaccagtaa atcacgcgcg cactgctgcg acagacttag ggtaaattcg aatttcagaa
     7381 aaagccatct gatattctgc aacgctcggc agtggcactg cgcgcagtcg aacaaatccg
     7441 gtatgatctt gctgttccta ttacccccta aaggagattg gtcgatgaca agatcgctga
     7501 ttaagacagt ggcgcggctg gttaagtggc gagcggttgg gctcgccacg ctggcgattg
     7561 tggcggccgg tgtgtctttc gctcaagccc ctgcaacgga aaagaagccg ggccaagcgc
     7621 agaagccgag cgcggctgag gagaagttgc ccaaggccga agcggttctc gacaagttgg
     7681 tcgaggccag cggcggcgaa gcggccatca acagaatcag gacccgtgta tcgaagggaa
     7741 cgtatgagca gggtccggcc aaggcaaaca tcacgatgta tgaagctgca cctaacaaat
     7801 cgcttcaaat cattgagctg ccggaagggc tgaaaatcga gcaaggtacg aatggcgagg
     7861 tgtattggac gcaatcgccg atgggtgtgc agattgctga caaggaagaa gctgccctgg
     7921 cggcgcggca gggcgacata cacctccctg caacctggcg ccggcactac aagagcgccg
     7981 aaacggccgg cattgagacg gttggcagta gccctgcgta caagctcatc atgacccccg
     8041 aggagggtcc gcccgaactg tggttcgttg accagaaatc caacctgctg gtgcgctggg
     8101 atatgcgcgc gggcgccggc atgggaaaca tctcgatata cttcgaagat caccggaaag
     8161 ttgacgacgt catggtacct ttcaggcagc gcgccgccgg cctgatgccg ctggtcgtca
     8221 ctttgagcag cgtcgagcat aacgttgaca ttcccaagga acggtttgag ctcccggaag
     8281 cggtgaagga agccctgaag gacgcaggga aggcgcctgc gctgcctggc catccgccgc
     8341 agcctccaaa acccgacagg ccggacaaac cggagaagcc gggcaaccca tgagcagcga
     8401 cagacacg
//
LOCUS       DAOSYS010000160         8380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7241_length_8380_cov_5.996517, whole genome shotgun sequence.
ACCESSION   DAOSYS010000160 DAOSYS010000000
VERSION     DAOSYS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7241_length_8380_cov_5.996517"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..391
                     /gene="rpsS"
                     /locus_tag="PLU99_10445"
     CDS             119..391
                     /gene="rpsS"
                     /locus_tag="PLU99_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOO17516.1"
                     /translation="MARSAKKGPYVNEHVFNKVLAAQKMGDRTPIKVWSRSCTIVPEF
                     VGVTFEVHNGRHFQKVFVTEDMVGHKLGEFSPTRTFRAHTARAVAK"
     gene            407..736
                     /gene="rplV"
                     /locus_tag="PLU99_10450"
     CDS             407..736
                     /gene="rplV"
                     /locus_tag="PLU99_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOO17517.1"
                     /translation="MWTAKHRYARIADRKARLVVDLIRGRRCDDAVELLRFTHKRAGK
                     LVDRVLKSAMASANEAEASMHRLVVSEARVDPGPIIKRFHPKDRGRAHPIMKRTSHIT
                     ISVSEKE"
     gene            740..1432
                     /gene="rpsC"
                     /locus_tag="PLU99_10455"
     CDS             740..1432
                     /gene="rpsC"
                     /locus_tag="PLU99_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOO17518.1"
                     /translation="MGQKCNPIGFRVGITEGWKSRWYAPKAAFGAFLVEDENIRRFIE
                     KELNEKPPFAGISKVEIERTRDEVKVILHSARPGVVIGAKGAEVDKLKERLEDLIDRR
                     VSVNIVEIKNPDLDARLVGQGIAEQLKKRGSFRRIMKMKCEAAMAAGAKGVKIICSGR
                     LGNAEMARTETQMLGSIPLQTLQAEVDYAITHAVTTVGVIGIKVWIYRGSYGEQPATA
                     AGDGRFRRRARR"
     gene            1573..1995
                     /gene="rplP"
                     /locus_tag="PLU99_10460"
     CDS             1573..1995
                     /gene="rplP"
                     /locus_tag="PLU99_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOO17519.1"
                     /translation="MPGNMPKRVKYRKAFKGRIRGNALRGNSVAFGDYGLQSLEPARI
                     TSRQIEAGRVAATHFLHREGRVYIRIFPWKPISKKPLETRMGKGKGEVDIWAAVVRPG
                     MVMYEVGGVGEDVAKAALLRVAHKLPVRTRFIARRHSV"
     gene            2005..2253
                     /gene="rpmC"
                     /locus_tag="PLU99_10465"
     CDS             2005..2253
                     /gene="rpmC"
                     /locus_tag="PLU99_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOO17520.1"
                     /translation="MALTIENLRAMKADELHAELERLRRHFFDLRAQAVTEKLEDPTQ
                     LGKTRRSIARVLTVLRERGEKNIEQRQDHLTAQAARHK"
     gene            2387..2653
                     /gene="rpsQ"
                     /locus_tag="PLU99_10470"
     CDS             2387..2653
                     /gene="rpsQ"
                     /locus_tag="PLU99_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOO17521.1"
                     /translation="MGVVASAARQKTIKVTVSYLVKHGKYGKFLSRRTVMHAHDENNE
                     AGVGDLVEVAECRPISKTKNWRLVRIVQRAPRQAAEVLGQGGEA"
     gene            2650..3039
                     /gene="rplN"
                     /locus_tag="PLU99_10475"
     CDS             2650..3039
                     /gene="rplN"
                     /locus_tag="PLU99_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOO17522.1"
                     /translation="MITQYTRLDVADNTGAKELQCIQVLKGSAARHGKKRLAKGGVGD
                     IAICSIKSVLPSSELKKGEKVRVVIVRTKYPTRRKDGSYVRFDSNAAVIIDGDGNPKG
                     TRVFGAVARELREKNFAKIISLAEEVW"
     gene            3042..3413
                     /gene="rplX"
                     /locus_tag="PLU99_10480"
     CDS             3042..3413
                     /gene="rplX"
                     /locus_tag="PLU99_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015085164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOO17523.1"
                     /translation="MAARVRKDDIVEVIAGEHRGARGKVLRVDPVKDRVVVEGVNMVY
                     RHVRKSRKHPQGGRIQKEAPIHASNVMPVDPKTGRGTRVRFQVQRDATGKVVSKQRLS
                     LGGSVLSEVTRATGEYKRRNQ"
     gene            3413..3973
                     /gene="rplE"
                     /locus_tag="PLU99_10485"
     CDS             3413..3973
                     /gene="rplE"
                     /locus_tag="PLU99_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOO17524.1"
                     /translation="MVRLLDKYRQTVIPALQQELGRQNIMSLPKLEKIVVSMGLGKAV
                     QESGNKSRENRRFTEAEEHLRTVTGQKPLVTKARKSVSNFKVREGWDVGLKVTLRGAR
                     MYEFLDRLVALAIPRVRDFRGLDPGGFDGRGNYNMGLSEYGVFPEIDPDKVNYQQGMN
                     ITICTSARNDAEARRLLALMGMPFRS"
     gene            3981..4166
                     /locus_tag="PLU99_10490"
     CDS             3981..4166
                     /locus_tag="PLU99_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOO17525.1"
                     /translation="MARLGLIHKSKRTPKYRVRAYTRCELCGRSRAVYRKFRLCRLCF
                     RKLAGEGRIPGVRKASW"
     gene            4166..4564
                     /gene="rpsH"
                     /locus_tag="PLU99_10495"
     CDS             4166..4564
                     /gene="rpsH"
                     /locus_tag="PLU99_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOO17526.1"
                     /translation="MATSDPIADMLTRIRNADRIGKRRVRVKRSKVCLGIAQVLKDEG
                     YILDFQQIEDTNQGEIEIDLKYADDGTHVIQDLQRVSRPGRRIYAHVDEMPRVLDGLG
                     ITIVSTSRGVLSDRQCRKQRVGGELLCKVW"
     gene            4569..5165
                     /gene="rplF"
                     /locus_tag="PLU99_10500"
     CDS             4569..5165
                     /gene="rplF"
                     /locus_tag="PLU99_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOO17527.1"
                     /translation="MSRIGKKPVPVPPGVKVNLSGRNVEITGPKGKLSWQVPEPITVA
                     LKDNQVVCDRPDDQKRSRALHGTSRALIANMVKGVAEGYRIDLEIYGTGYGCKLEGKT
                     FLLNVGYMGRGIGRKAQFEIPVPPGLEIKVDAPVARGDNEPAKFSVSGPDKQQVGAFA
                     ADIRKLRKPEPYKGKGIRYAGEHIRRKAGKVFAGGGGG"
     gene            5197..5565
                     /gene="rplR"
                     /locus_tag="PLU99_10505"
     CDS             5197..5565
                     /gene="rplR"
                     /locus_tag="PLU99_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOO17528.1"
                     /translation="MRTEFLKVARQERRIRRVRKKVDGTPARPRLAVSRSNTGIYAQI
                     IDDVARRTLCGVSSRGKEIREQVTYGGNVKAAAVVGKALAEKARALGIEQVCFDRRGR
                     KYHGRIKALADAAREAGLKF"
     gene            5588..6121
                     /gene="rpsE"
                     /locus_tag="PLU99_10510"
                     /pseudo
     CDS             5588..6121
                     /gene="rpsE"
                     /locus_tag="PLU99_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            6256..6699
                     /gene="rplO"
                     /locus_tag="PLU99_10515"
                     /pseudo
     CDS             6256..6699
                     /gene="rplO"
                     /locus_tag="PLU99_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006736157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            6848..8245
                     /gene="secY"
                     /locus_tag="PLU99_10520"
     CDS             6848..8245
                     /gene="secY"
                     /locus_tag="PLU99_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOO17529.1"
                     /translation="MLRAFINTFRIPDLRNKVLFTLFMLSVYRIGYYVPLPGVDQETL
                     SSHFRGGASGSPVGQMADLFALFTGGDLGQSTLFGLGIMPYISASIILQLLVTVVPAL
                     EKLQKEGESGRRKINEYTRYLTVALCLIQSVFWVQYLHSNNLVYAEFSTGMMWWTFAL
                     MAIVGLMAGTMLLMWIGEQIDEYGIGNGISLIIMAGIIARLPTVFIEIYSGLFRSGQM
                     AGATYITPEKVVFLVLSFVAVVAGAILITQAQRRIPIQQAKQTRGRRIVGGARHYLPL
                     RVNHGGVMPIIFASSLLMFPQFILGWLANANWPSWLAWAGTVASQVNANFHMGSYLYE
                     VSYIAMVYFFAYFWTAVQFQPKELANNLRDMGSFIPGLRPGKRTADYLEAVMQRITYV
                     GAGFLALIAVIPSVVATQLEIPWRISAFLGGTGLLIVVSVALDFINRIEANLIMRNYE
                     GFLGHEGVARVKGMY"
     gene            8245..>8380
                     /locus_tag="PLU99_10525"
     CDS             8245..>8380
                     /locus_tag="PLU99_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOO17530.1"
                     /translation="MADKRLVFLGPPGSGKGTQAQRLCDRLGLTQLASGDLLRREVRE
                     K"
BASE COUNT         1673 a         2416 c         2872 g         1419 t
ORIGIN      
        1 ggacgcgcag tccgcgggcg cgttcgaatc gccggattct gcgtcgccgc ataacgaagt
       61 catttggcga ggcgaagctg cggcggaagt ggtaaggcag ttagctagag gaatacgcat
      121 ggcgcgtagc gcgaaaaaag gcccgtacgt aaacgagcac gtgttcaaca aggtgctcgc
      181 ggcccagaag atgggcgacc ggacgccgat caaggtgtgg agccggtcgt gcacgattgt
      241 gccggaattt gtgggcgtga cgttcgaggt gcataacgga cggcacttcc agaaggtgtt
      301 cgtgaccgaa gatatggtcg ggcacaagct gggtgaattc tcgccgacgc ggacgttccg
      361 ggcgcacact gcacgcgcgg tggcgaagta gcaggaggcc ttgaagatgt ggaccgcaaa
      421 acaccgctac gcacggatcg ccgatcgcaa ggcgcggctc gtggtagatc tgatccgcgg
      481 gcggcggtgc gatgacgcag tggagttgtt acgcttcacg cacaaacgcg ccggtaagtt
      541 ggtcgatcgc gtgctcaaat ccgcaatggc cagcgccaat gaggctgagg cgtccatgca
      601 ccggctggtc gtaagcgaag cacgggtcga tccgggtccg atcatcaagc gctttcaccc
      661 gaaagaccgc gggcgggcgc acccgatcat gaagcggact tctcacatta caatttcggt
      721 gagtgaaaag gaataacgcg tgggccagaa gtgcaatcca atcggcttcc gggtgggcat
      781 taccgaaggc tggaagtccc gctggtacgc ccctaaagcg gcctttggcg cgttcctcgt
      841 cgaggatgag aacattcgcc gcttcatcga aaaggaactc aacgagaagc cgcctttcgc
      901 cggcatctcc aaggtcgaaa tcgagcgtac gcgcgacgaa gtaaaggtga tcctgcactc
      961 cgcccgcccc ggcgtcgtga tcggcgccaa aggggccgag gtggacaagc tcaaggaacg
     1021 cctggaagac ctgattgatc gtcgcgtctc cgtgaatata gttgaaatca agaaccccga
     1081 tctggacgcc cggctggtcg gccagggcat cgccgagcag ctcaagaaac gcggcagctt
     1141 ccggcgcatc atgaagatga agtgcgaggc cgccatggcg gccggggcca agggcgtgaa
     1201 gatcatctgc tccggccggc tgggcaacgc ggaaatggcc cgcaccgaaa cgcagatgct
     1261 gggttcgatt ccgctgcaga cgttgcaggc ggaagtggat tacgccatca cgcatgcggt
     1321 aaccacggtc ggcgtgatag gcatcaaggt ttggatttat cgcggcagtt atggcgagca
     1381 gccggcgacg gcggccggag acggccggtt ccgccgccgg gcgcgtcgct aacgggcgtt
     1441 ggttttaggt gtgctagtgt tggaggtctg agtctccgcg tggcacacgg cactattgag
     1501 gcttagaccg cactgttgag gctcaggcct cacagtggca cacggtagag tggcacacgg
     1561 taggagttag ttatgccggg caacatgccg aaacgcgtga agtaccgcaa ggccttcaag
     1621 ggccgcatcc gcgggaacgc gctgcgcggc aacagcgtcg cctttggtga ctatggtttg
     1681 cagtcgctgg agccggcgcg aataacgtcg cggcagattg aggccgggcg cgtggcggcg
     1741 acgcactttc tgcaccgcga aggccgggtt tacatccgca tcttcccctg gaagcccatt
     1801 tcgaagaagc cgctggaaac gcgtatgggc aagggcaagg gcgaggtgga catctgggcg
     1861 gccgtggtcc ggccgggcat ggtaatgtac gaggtcgggg gtgtcggcga ggacgtggcc
     1921 aaggcggcgt tgttgcgggt ggctcacaag ctaccggtgc gcaccaggtt catcgcccgc
     1981 cggcatagcg tgtaggagcc ggtcatggcg ttgacgatcg agaatttgcg ggccatgaag
     2041 gcggatgagc tgcacgcgga gctggagcgc ctgcggcggc actttttcga cctgcgggcc
     2101 caggcggtga ccgagaagct ggaagacccg acgcagctcg gcaagacccg gcgcagcata
     2161 gcgcgcgtct tgacggtctt acgtgaacgc ggcgaaaaga acatcgaaca gcggcaggac
     2221 catctgacgg cccaggccgc gcggcataag tgagttggta gtgcaaagca aagctccgct
     2281 ttgccggcac tgattgcaga acggatagca cggaatggat acgacagaac ccggaaaagc
     2341 cgtggtgtca ccgcctgcgg cgccgcgcgc gcggcgtgcc acgcgcatcg gcgtggtcgc
     2401 gtctgcggcg cgccagaaga ccatcaaggt gacggtttct tatctggtca agcacggcaa
     2461 gtacggcaag tttctgagcc ggcgcacggt gatgcacgcc cacgacgaga acaacgaggc
     2521 cggggtgggc gacctggttg aggtggccga gtgccgcccg atcagcaaga ccaagaattg
     2581 gcgcctggtg cggatcgtgc agcgggcccc gcggcaggcg gccgaggtgc tggggcaggg
     2641 gggtgaagcg tgattacgca gtacacgcgg ctggacgtgg ccgacaacac gggcgccaag
     2701 gagctgcagt gcatccaggt gctgaagggt tcggcggccc ggcacggcaa gaagcggctg
     2761 gccaagggcg gggtggggga catcgccatc tgctcgatca agagcgtgtt gccatcatcg
     2821 gagctgaaaa agggcgaaaa ggtgcgcgtg gtgattgtgc gcaccaagta cccgacgcgc
     2881 cgcaaggacg gcagctacgt gcgtttcgac agcaacgcgg cggtgattat tgacggcgac
     2941 gggaatccca agggtacccg cgtattcggg gcggtggccc gcgagctgcg cgagaagaac
     3001 ttcgccaaga tcatctcgct ggctgaggag gtttggtaag tatggccgcg cgggttcgta
     3061 aagacgacat tgttgaagtg attgccggcg agcaccgggg tgctcgcggc aaggtgctgc
     3121 gggtcgatcc ggtcaaggac cgggtggtgg tcgaaggcgt taacatggtt tatcgccacg
     3181 tgcgcaagag ccgcaagcac ccgcagggcg gtcgaattca gaaagaggcc ccgattcacg
     3241 cctcaaacgt gatgccggtc gatcccaaaa ccgggcgcgg tacgcgggtg cgcttccagg
     3301 tgcagcggga tgccacgggc aaagtggtca gcaagcagcg cctcagcctc ggcggctccg
     3361 tgctgagcga agtgacgcgc gccaccggcg aatataagcg caggaatcag taatggtacg
     3421 gctactcgac aaataccggc agacggtcat accggctctg cagcaggagc tggggcggca
     3481 gaacatcatg tcgctgccca agctggagaa gatcgtggtt tcgatgggcc tgggcaaggc
     3541 ggttcaggag agcggcaaca agtcgcgcga gaaccgccgc ttcaccgagg ccgaagagca
     3601 tctgcgcacg gtaaccgggc agaagccgct ggtaaccaag gcccgcaaga gcgtatcgaa
     3661 cttcaaagtt cgcgagggct gggacgtcgg gctgaaggtg acgctgcgcg gggcgcgaat
     3721 gtacgaattt ctcgatcgcc tggtggcgct ggccatcccg cgcgtccgcg attttcgcgg
     3781 tctggacccg ggcggcttcg acgggcgcgg caactacaat atgggcttgt cagaatacgg
     3841 cgtatttccg gagatcgatc cggacaaagt caattatcag cagggtatga acattaccat
     3901 ttgcaccagc gcccgcaacg acgccgaagc gcggcggctg ctggcgctga tgggcatgcc
     3961 cttcaggagc taagcggtca atggccagat tgggactgat acacaaatcc aagcgcacgc
     4021 cgaagtaccg cgtgcgggcg tacacgcgct gcgagctgtg cggccgctcg cgtgcggttt
     4081 atcgcaagtt ccgcctgtgc cgcctgtgct tccgtaagct ggccggtgaa ggtcgcatcc
     4141 ctggggtgcg gaaagcgagt tggtaatggc cacatcggac ccgatcgccg acatgctgac
     4201 acgcatccgt aacgccgacc ggatcggcaa gcggcgggtg cgcgtcaagc gcagcaaggt
     4261 gtgcctgggg attgcccagg ttctcaagga tgaaggctat attctggact tccagcagat
     4321 cgaggacacc aaccagggcg agatcgaaat cgacctgaag tacgccgacg acggcacgca
     4381 cgtgatccag gatctgcagc gcgtcagccg gcccggacgg cgcatctatg cgcacgtcga
     4441 cgagatgccg cgcgtgctgg acggtttggg cataacgatt gtctccacca gccgcggcgt
     4501 gctgagcgac cggcagtgtc gcaagcagcg cgtgggtggc gagttgctct gcaaggtttg
     4561 gtgattcgat gtcgcggatt ggcaagaaac cggttcccgt cccgcccggc gtcaaggtca
     4621 acctctccgg gcgaaacgtg gaaatcaccg gtcccaaagg caagttgagc tggcaggtcc
     4681 ccgagccgat taccgtggct cttaaggaca accaggtcgt ctgtgaccgg cccgacgatc
     4741 agaagcgcag ccgggccttg cacggcacct cgcgcgcctt gatcgcgaac atggtgaagg
     4801 gcgtggccga gggctatcgc atcgacctgg aaatctacgg caccggctat ggctgcaagc
     4861 tcgagggcaa gaccttcctg ctgaacgtcg ggtacatggg gcggggcatc ggtcgtaagg
     4921 cccagttcga aattccggtg ccgcccgggc tggaaatcaa ggtcgacgcc ccggtcgccc
     4981 gtggcgacaa cgagcccgcg aaattctcgg tcagcggccc agacaagcaa caggttggcg
     5041 catttgcggc cgacatccgt aagctccgca agccggaacc ctacaagggc aagggcatcc
     5101 gttacgccgg cgagcacatt cgccgcaagg ctggcaaggt gtttgcgggc ggcggcggcg
     5161 gctgattggg ctgtgattcg gctctgaggt aaggtaatgc gaacagaatt cctcaaggtg
     5221 gcacgccagg agcgacgaat tcgccgcgtg cgcaaaaagg tggacggtac tccggcccgg
     5281 ccgcgcctgg cggtcagccg ctccaatacg ggcatttacg cccagattat tgacgacgtt
     5341 gccagacgca cgctgtgcgg cgtctccagc cgcggcaagg aaattcgcga gcaggtgacc
     5401 tacggcggaa acgtcaaagc ggcggccgtc gtgggcaagg cgttggccga aaaagcgcgg
     5461 gccctcggca tcgagcaggt gtgtttcgat cgccgcggcc gcaagtatca cggccgaatc
     5521 aaggctttgg cggatgcggc ccgcgaagcc ggtttaaagt tctagggcaa aggcagagaa
     5581 ggtaattatg tcgacacggc cgaaacaagc gcgcggcgga ggcggcggcg gcagacggcg
     5641 gctagcggac catgagcccg gcgccgaatc caacattgat gacgcgctgg tcaagcttta
     5701 ccgctgtgcc tgcgtggtga agggtggccg tcgcttttcg ttcggcgccc tggtggtcgt
     5761 cggcgaccgg cagggtcgcg taggctatgg ttacggcaag gccaacgagg tcccgccgtc
     5821 cgtcgagaag ggcaacaagc aggctcgccg gcgcatgttc accgttccac tgcggggcac
     5881 gacgataccg caccgcgtgg tgggcgcgta cggcgccagc cgggtggtgt tgatcccggc
     5941 cgccgaaggt accggcgtca ttgccggctc ggctccgcgg gccgtgctgg aactggccgg
     6001 gattaaggac gtgctgacca aggtctacgg ctcgacgagc ccgaagaacc tggtgaaagc
     6061 caccatcgac ggtctccaga aactgcgctc gcgggcgcgg gtcgaggagt tgcgcggggt
     6121 cacactggat cttccgccgg agcgcccatc gaaaaaggcg cccatcgagg cgtatgtctc
     6181 ggccgatgag ccttccgatg agggcgaggc ggacagcggc caggccactc cgcaacagca
     6241 gcaggaaatg cagccatgat gctgaacgaa ataacagcga cggcgggggc gcaccggccg
     6301 cgtaagcgcg tcgggcgagg cgaagccagc gggcacggca agacctgcgg gcgcggcaac
     6361 aagggctttc agtcgcgcgc cggcgggggc gcgcggcgcc tgaccgaagg cggtcagatg
     6421 ccgctgttcc ggcgcttgcc gaagcgcggc ttcagcaatt tcgagtttcg caccgagtac
     6481 caggtggtca acgtgggagt gctgaacgag cactttgacc agggcggacg cgtggatctg
     6541 gagagcctgc gaaagctgaa cctggtgcag ggtccgcgac cgttggtccg catcctgggg
     6601 cacggcaagc tggacaagaa gctgaccgtt gcggcccatg cgttcagcgg ccaggcgcgg
     6661 gcggcgatcg agcaggcggg cggaaccgtg caggttattg agcgtcccga ccgggccgca
     6721 caagcacggg ctaaacgaaa tacggccaag aagtcgtccc ccaaaaagtc cgccccgaag
     6781 aagggcgaca cggcgccacc ggccggcgca tagcaaaggg cgcgagtccc aagaaggttg
     6841 aactgagatg ctgcgtgcct ttattaatac cttccggatt ccggacctcc gcaacaaggt
     6901 gctgtttacg ctcttcatgt tgtcggtata ccggattggt tactacgttc cgcttccggg
     6961 agtcgatcag gaaactcttt ccagtcactt ccgtggcggc gccagcggca gcccggtcgg
     7021 ccagatggct gatctgtttg ccctgttcac cggcggcgac ctggggcaga gcacgttgtt
     7081 cggcttggga atcatgcctt acatcagtgc ctcgatcatt ctgcagttgc tcgtgacggt
     7141 tgtgcctgcc ctggaaaaac tccaaaaaga gggtgaatcc ggacggcgaa aaatcaacga
     7201 gtacacccgc tacctcaccg tcgccttgtg tttgatacag tcggtgttct gggtgcagta
     7261 cctccacagc aacaacctgg tgtacgccga attctcgacc ggcatgatgt ggtggacatt
     7321 cgccctgatg gccatcgtgg gtttgatggc cggaaccatg ctgctgatgt ggatcggtga
     7381 gcagatcgac gagtacggca tcggcaacgg catcagcttg atcatcatgg ccggcatcat
     7441 tgcccgcttg ccgaccgtgt ttatcgagat ctacagcggc ctcttccgca gcggccagat
     7501 ggcgggtgcg acctacatca cgccggaaaa ggtggtcttc ctggtcctgt cgttcgtggc
     7561 ggtcgtggcc ggggcaatac tgattaccca ggcccaacgc cgcattccga ttcagcaggc
     7621 caagcagact cgcggccggc gcattgtggg cggggcgcgg cactacctgc cgttgcgcgt
     7681 gaaccacggc ggcgtgatgc ccatcatttt cgccagctcg ttgctgatgt tcccgcagtt
     7741 cattctggga tggctggcga acgcgaattg gccgtcctgg ctggcctggg ccggcaccgt
     7801 cgccagtcag gtgaacgcga acttccacat gggcagctat ctgtacgaag tgtcgtacat
     7861 cgcgatggtg tacttcttcg cgtacttctg gacggccgtc caattccagc ccaaggaact
     7921 ggccaacaac ttgcgcgaca tgggcagctt catccccggc ctgcggcccg gaaagcggac
     7981 cgcggattac ctcgaagccg tcatgcagcg gatcacttac gtcggcgccg gcttcctggc
     8041 cctgatcgcg gtgatcccgt cggtcgtcgc gacgcagctt gaaatcccgt ggcgcatctc
     8101 ggccttcctg ggtggcaccg gcttgctgat cgtcgtcagc gtcgccttgg acttcattaa
     8161 ccgcatcgaa gccaacctga tcatgcgcaa ctacgagggc ttcctcgggc atgaaggcgt
     8221 ggcgcgggtc aaaggaatgt actaatggct gacaagcgac tggtctttct cggtccgccc
     8281 ggcagcggca aaggcacgca ggcccagcgc ctgtgcgacc gcttgggtct gacgcaactc
     8341 gccagcggcg acctgctgcg gcgcgaggtt cgcgaaaaga
//
LOCUS       DAOSYS010000161         8368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7259_length_8368_cov_5.343558, whole genome shotgun sequence.
ACCESSION   DAOSYS010000161 DAOSYS010000000
VERSION     DAOSYS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7259_length_8368_cov_5.343558"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLU99_10530"
     CDS             <1..730
                     /locus_tag="PLU99_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17531.1"
                     /translation="GVFPEGISRSRGRAIIHRYFQRTRCDKLYYLIFDRLPRDKIMRR
                     IKFQGREHVDAALARGNGLYTMLSHNGSHHIAGLLMALCGYRVAGIRDRNEGAIRMYV
                     QERYAETFPEYAAIRVLYADSFPREIYRCFQENRVVGSALDVSRVRGLTLKTCPVNIF
                     GEQREFLTGTLQIALRCRATVCQAFVVSRPNFYFRLIACPPLYVPGEGESEESPELIQ
                     RIMQQYADGIAAHVREHPDHLSRV"
     gene            791..1240
                     /gene="dtd"
                     /locus_tag="PLU99_10535"
     CDS             791..1240
                     /gene="dtd"
                     /locus_tag="PLU99_10535"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOO17532.1"
                     /translation="MRAVVQRVSQAGVTVAGEVVARIGPGLLVYAAAAPDDSADDVRY
                     TAEKLAHLRIFPDEAGKMNLDVTQVKGDVLLVSAFTVYADARKGRRPSFDACARGEVA
                     EPLVSALARALRGFGLTVESGRFGAHMLVESVNDGPICILLDSRRTF"
     gene            complement(1296..1997)
                     /locus_tag="PLU99_10540"
     CDS             complement(1296..1997)
                     /locus_tag="PLU99_10540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO17533.1"
                     /translation="MTSLLELDGVFLDMYGTLTAGDRAAVESVCTEVVALTGVPLSPV
                     ELSVRWGVRYFAALECCNDGQFCNLFEVEARTLRETMRELGVDLDPYPFAQALTAYWR
                     DPPLQPEALEFLSEFKAPICLVSNADRADIEAALARHGIEIPFIVTSEDVRCYKPAAR
                     IFAEALRRTGWRRERVIHVGDSLHSDVGGALAAGLRSGWVNRAHRIHDVGNHVPDYEF
                     ENLRGLMALVTAVGR"
     gene            complement(2038..2529)
                     /locus_tag="PLU99_10545"
     CDS             complement(2038..2529)
                     /locus_tag="PLU99_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOO17534.1"
                     /translation="MAISEPMNAKLNEQITNEFNAEHTYLSMACMFDGLGLSQLAMYF
                     RKQADEERGHALKILDYLLEAEGTVKLQGVPQPPHAWDSVLAAIEAALEHERRVTAQI
                     HSLVELAEGEKDYATRTFLNWFVEEQVEEVASMVRLRDVAKLADPSLLMIDMYVGHAI
                     KRD"
     gene            3073..5028
                     /locus_tag="PLU99_10550"
     CDS             3073..5028
                     /locus_tag="PLU99_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HOO17535.1"
                     /translation="MRVGLRAILCGPVAALLVAAAAWPAQAAGIEQFKLAGAIPADAT
                     MAVYCRDHAGREFIEAQLQRVWKTVEAQHFDRELRILIKSLYESGTMPLDEAERQKRD
                     EEFEAQWQRFDALLKAVKWSDLFQREGAFGMKVGFPTPELVVMGMGPPERTAANFAGL
                     EAILAALVSFAPEGALVSSRQGEGEAVVLTVALGPDSPLPLSLMLARQKDVLMLGFGQ
                     VMPEQALALLRGETGQTLASTERFQSALKRLPPPTDSVFFLDMTRLLGQVRGVIDAAI
                     GMAGPAGEQLDPMQRALPGKFLDAFDIVDYIVTVTDTQGMKSTTESLVQLLPNAESKP
                     LYPVVFGNQAVKEPLKFIPQTAQDFSVWAGFNLKALYDAIITFVRDNVPNGSELLTQW
                     ETTKTTLPFDIEQDVLSWVGGSMITFSLPAETAYKPGGWFFATNVTDEAKAQESLTRL
                     FNWLDTYLREQQNGALRDAEVEGATGFKTIVHPMLIVMGGAGQPTIGIAEGHLIVASG
                     PKVISTALATAKGDSPNFSKNERFLKEGLRLPPDVWAASFSDLTKLGEQMGQALKMVP
                     MMGMFMPSMGQTPAGRALMSAASKLGNVVQEINFLESSSSVSTFDGKAVHSKAVWNYR
                     EPPPPQTQPAPVEEEPPATQPVAGSRS"
     gene            complement(5160..5819)
                     /locus_tag="PLU99_10555"
     CDS             complement(5160..5819)
                     /locus_tag="PLU99_10555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO17536.1"
                     /translation="MAPNGPSACIFDLDGTLVDSLQDIAESLNECLALLGLPPRPVED
                     YRYLVGEGIPTLAQRLLGDTNPLLAARLTELARARYRTRPLLHTRPYREVDALVQQLR
                     AAGLRLAVLSNKPHELTVRVVRAFWPDSFDAIQGYVEEDLRKPNPLYALRLCERMQVQ
                     PQQVWFIGDTPTDVETAHRAGAVSIGVTWGFRTRADLEAAGADRVFDEPLEIARQALS
                     R"
     gene            5933..7249
                     /locus_tag="PLU99_10560"
     CDS             5933..7249
                     /locus_tag="PLU99_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17537.1"
                     /translation="MFLRTSGKLLLVVALAALAGVVPARSQSAERSARSFASLINVDA
                     LIDNYLRFVARKYNLTEEQDQYTEQLVRARVDEFLERHYDELADLVDKLVMVRNGGDV
                     APEELIEWGRRVQPLYVEAKGIIIQTNDEWRGILTEEQRRMHDEDIRLMWESFSTTED
                     QLSRLTAGQMSVEEFRNPRIARQGRAQAAAAQPGVTASAERAGSALRQAKAAAAADQG
                     VSQKRAAAKGAGGGPAAGRSSAAVPDRGSKRTPGKRSAAGATSADEWEQYVQQFIQRY
                     ALTEEQASRANKILKSCQSQRDRLMRNRAEQLERLNRQEEALRTSSDNPNKAKEQAEI
                     TAQRAKLMAPIDSIFDKQLKPRLEKIPTRAQREAAEKAPAAGRTAPGKPASVVPGKSA
                     APRVGKPGAPPTLPKKPVPPAPPPDSEGSPDNPEEEEDYEEEGENK"
     gene            7469..7711
                     /locus_tag="PLU99_10565"
     CDS             7469..7711
                     /locus_tag="PLU99_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17538.1"
                     /translation="MRACLRRGRMWLLCTAIGGVAVLEGCDASVRADVLSGVGSAATG
                     LATTFINAFFTGLINKQSDDAPVVQRFDQSELRVFC"
     gene            8037..>8368
                     /locus_tag="PLU99_10570"
     CDS             8037..>8368
                     /locus_tag="PLU99_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HOO17539.1"
                     /translation="MAAGDLDVGDRNVHGYGSAKVASTINVLAGIWLIISPFVLRFWH
                     VSAAAWDNIIVGVIVLVLAAIRVAQPTRNLALSWINLILGIWLFISPWVLRFSHERAA
                     TSNDVVLG"
BASE COUNT         1569 a         2561 c         2732 g         1506 t
ORIGIN      
        1 gggggtcttt ccggaaggga tctcgcgttc gcgcggccgg gccataattc atcgctattt
       61 tcagcgcacc cgctgcgaca agctgtatta cctgatcttc gaccggctgc cgcgcgacaa
      121 gatcatgcgc cgcatcaaat tccagggccg cgagcacgtg gacgcggccc tggcccgcgg
      181 caatggcctg tataccatgc tcagccacaa cggctcgcac catatcgcgg ggctgctgat
      241 ggctctgtgc ggctatcgcg tcgccggcat tcgcgaccgg aacgagggtg cgatccgcat
      301 gtacgtgcag gagcgctacg ccgagacttt cccggagtac gcggccatcc gggtgctgta
      361 cgcggattcg ttcccacgcg agatttaccg ttgcttccag gaaaaccggg tggtcggcag
      421 cgcgctggat gtcagccgcg tgcgcggcct gacgctgaag acctgcccgg taaatatctt
      481 cggcgaacaa cgcgagtttc tgaccggcac gctgcagatc gccctgcgct gccgggccac
      541 ggtgtgccag gcgtttgttg tttcgcgccc gaatttttat ttccggctga tcgcctgccc
      601 gcccttgtac gtgccgggcg agggcgagtc cgaagagagc ccggagctga tccagcgcat
      661 catgcaacag tacgctgacg gcatcgcggc gcacgtgcgg gaacatccgg accatctcag
      721 ccgagtctga cgtgggcgct gagtgtcgtt gccgcaaacc acttcagtaa tcactggagg
      781 acggtaacag gtgagagccg tcgtgcagcg cgtcagtcag gccggtgtga ccgtggccgg
      841 cgaggtcgtc gcacggatcg ggccggggct gctggtctac gccgccgcgg cccccgacga
      901 ttcggccgac gacgttcgct acacggccga gaagctggcc cacctgcgca ttttcccgga
      961 tgaggccggg aagatgaacc ttgatgtgac gcaggttaag ggggacgtgc tgctggtcag
     1021 cgcgtttacc gtctatgccg acgcgcgtaa ggggcggcgg ccgtcgttcg acgcttgcgc
     1081 ccgcggcgaa gtagccgaac cgcttgtcag cgcgctagcc cgcgcgctgc gcggcttcgg
     1141 actgacggtc gagagcgggc ggttcggggc gcacatgctg gtcgaatcgg tgaatgacgg
     1201 gccgatctgc atcctgctgg actcgcgccg caccttttga gcagagccgg agcgtgctga
     1261 tcagagccgg ggcgtggcgc cccggaatac ctctgctacc tgccaaccgc cgtaaccagc
     1321 gccatcagtc cgcgcaggtt ctcgaactcg tagtccggga cgtggttgcc cacatcgtga
     1381 atgcggtggg cacggttcac ccagccgctg cgcagtccgg ccgccagtgc tccgcctacg
     1441 tcactgtgca atgaatcgcc cacgtgaatg acgcgttcgc ggcgccagcc cgtgcgtcgc
     1501 aacgcttcgg cgaatatgcg cgccgccggc ttgtagcaac gcacgtcctc ggacgtgacg
     1561 ataaacggaa tctcgatgcc gtgccgcgcc agcgccgctt cgatgtccgc gcggtccgca
     1621 ttggagacca ggcagatcgg cgccttaaac tcgctcagaa actcgagcgc ctccggctgc
     1681 agcggcggat cgcgccaata ggccgtcaaa gcctgggcga agggataggg gtcgaggtcc
     1741 acccccagct cacgcattgt ttcgcgcagg gtccgcgcct cgacctcaaa gaggttgcag
     1801 aactgcccgt cgttacagca ctccagcgcc gcgaagtaac gcacacccca ccgaacgctc
     1861 agctccacgg gcgaaagcgg cacgccggta agcgcgacga cctccgtgca cacgctctcc
     1921 accgccgccc ggtctccggc ggtaagcgtg ccatacatgt ccagaaaaac gccgtcgagc
     1981 tctagcaaac tggtcaactt gcgttcgtat gggcctgcaa ctcgccggcc ggctccacta
     2041 atccctcttg atcgcgtgcc caacgtacat atctatcatc agcagcgacg ggtctgccag
     2101 cttcgccacg tcgcgcagcc gcaccatcga ggcgacctct tccacttgct cctcgacgaa
     2161 ccagttcagg aacgtgcgcg tcgcgtagtc cttttcgccc tcggccagct cgaccaggct
     2221 gtgtatttgc gccgtcacgc gccgctcgtg ctccagggcc gcctcgatgg ccgccagcac
     2281 cgaatcccac gcgtgcggcg gctgcggaac gccctgcagc tttaccgtgc cctcggcttc
     2341 cagcaggtaa tccagaattt tcagcgcgtg cccgcgctcc tcatccgcct gcttgcggaa
     2401 atacattgca agctgtgaaa ggccgagccc atcaaacatg caggccatcg acaaataggt
     2461 gtgctcggcg ttgaactcgt ttgtaatctg ctcgttcagt ttggcattca tcggctcgga
     2521 tattgccatc tctcaccctt tcagttaacg ggagtttagc cctgcgtcgt ccgcgacctg
     2581 ccgctcgccg cgcggtcgcg cgtaaccttt tccgacaagc gattctatgg cgttactatt
     2641 ggttcagcca attcttttgc cacggccgtt gtaggcatat aagttctgtc tgggtagaga
     2701 cttgcgtgcg tctcgggcgc aaggggagcg tctgatggct ggcgtgacgc cggactgtca
     2761 aggcccgtgc gcgctcccgg cggcacctgc caacagttgt ccatcgcgcg cccggtcgcc
     2821 tgtgcaagtg acttgaccag tgaatgccgg acgcggtgcg cgtgctgcgc tcggctactt
     2881 ctggtcgcgc gggacgttgc actcaacggc agtcgaaaag ggaattttct cgccgcggcg
     2941 tcggaagttg tgaaaaacac gtcactgttg caacgctcgc ccttcctcga ccggccatgc
     3001 gatgtaatat taacaggatc ggcggccaac cctggcgcgg caggccgggc cgattctgag
     3061 caaggagatc acatgagagt tggattgaga gctattctgt gcggtcctgt tgcagcgttg
     3121 ctggtggcgg cggcggcctg gccggcgcaa gcagccggaa ttgagcaatt caagttggcc
     3181 ggcgccattc cggcggacgc caccatggcc gtctattgcc gcgatcatgc gggccgggag
     3241 ttcatcgaag cgcagttgca gcgcgtttgg aaaacagtcg aagcccagca ctttgacagg
     3301 gaactgcgga ttctgatcaa gtcgttgtac gagtccggta cgatgccgct ggacgaggcc
     3361 gagcggcaaa agcgcgacga agaattcgag gcgcaatggc agcgctttga cgcgcttttg
     3421 aaggccgtga aatggtccga cctgtttcag cgcgaaggcg ctttcggcat gaaggtcggt
     3481 ttcccgacgc ccgaactcgt cgtgatggga atgggaccgc cggagcgaac cgcggccaac
     3541 ttcgcggggt tggaggcgat cctggcggcg ctagtcagct tcgcgcccga aggcgccttg
     3601 gtatcgtcgc ggcaggggga aggcgaagcg gtcgttctca cggttgcgtt gggcccggat
     3661 tcgccgctgc ccttgagtct gatgctggcg cggcaaaagg atgtgttgat gctgggcttc
     3721 gggcaggtca tgcccgagca ggctctggcg ttgctgcgtg gcgagacagg gcagacgctg
     3781 gcgtcgacgg agcgctttca gtcggcgctc aaacgtctcc cgccgccgac cgattcggtt
     3841 ttctttcttg atatgacgcg gctgctgggg caggtgcgcg gggtgatcga tgcggctatc
     3901 ggaatggcgg gcccggccgg cgagcagctt gatcccatgc aacgggcgct gccgggtaag
     3961 ttcctggacg ccttcgacat agttgattac atcgtaaccg taactgacac gcagggcatg
     4021 aagagcacca ccgaaagctt ggtgcaactg ctcccgaatg ccgaaagcaa accgctatac
     4081 ccggtggtct tcggaaacca ggcggtcaaa gagccgctca agttcattcc ccagacagct
     4141 caggatttca gcgtgtgggc gggattcaac ctgaaggcgc tttacgatgc gatcatcacc
     4201 ttcgtgcgcg acaatgtgcc gaacggctcg gaactgctga cacaatggga gacgaccaaa
     4261 actaccctgc cgttcgatat tgagcaggac gtcctaagct gggtcggcgg gtcgatgatt
     4321 acgttctctc taccggccga gacggcgtac aagccgggcg gctggttctt cgcgaccaat
     4381 gtgactgacg aggccaaggc tcaggagagc ctgacccggc ttttcaattg gctcgatacg
     4441 tacctcaggg agcagcagaa cggcgcgctg cgcgacgccg aggttgaggg cgccaccggg
     4501 tttaagacca tcgttcaccc gatgttgata gtcatgggcg gcgccggcca accgaccatc
     4561 ggcatagccg agggccatct catcgtcgcc tccggtccga aagtgatctc gaccgcgttg
     4621 gccacggcca agggcgactc gccgaatttc tcgaagaacg agcgcttcct gaaggaaggt
     4681 ctgcggcttc cgccggacgt ctgggccgcc agcttcagcg atttgaccaa gcttggcgaa
     4741 cagatgggtc aggcgctcaa aatggtgccg atgatgggca tgttcatgcc gtcgatgggg
     4801 cagacccccg cgggacgcgc gctgatgtcg gccgccagta aattggggaa cgtggttcag
     4861 gagatcaatt tcctcgagtc atcgtcctcg gtgtccacgt tcgacggtaa agccgttcac
     4921 agtaaggccg tgtggaatta tcgcgagccg cccccgccgc agacccagcc ggctcccgtc
     4981 gaggaagaac cgccggctac acagccggtg gccggcagca ggtcttaggc aatagtcctg
     5041 agcgcggctc gggcgaggat ttcggtgggc cggcggcatt ccggggcgag ccgccggtcg
     5101 ccgaggcaac gccagttccg tccggctagg ctgcgcttcg cccggcttca agacaacgcc
     5161 tagcgggaca acgcctgacg ggcgatttcc agcggctcgt cgaaaacgcg atcggcgccg
     5221 gccgcctcca agtctgcccg cgtgcgaaat ccccaggtaa cgccgatcga tacggccccc
     5281 gcgcggtggg ccgtttccac atccgtgggt gtgtcgccga tgaaccagac ttgttgtggc
     5341 tgcacctgca ttcgttcgca gaggcgcagt gcgtacagcg ggttcggctt tcgcaggtct
     5401 tcttcgacgt agccttggat ggcgtcgaag ctgtcaggcc agaaggcgcg gacgacgcgc
     5461 accgtcagct catgcggctt attggacagg acggccagcc gcaggccggc agcccgcaac
     5521 tgctggacga gggcgtcaac ctcgcggtag gggcgggtgt gcaggagcgg ccgggtccgg
     5581 taacgagcgc gggctagctc ggtgagtctg gcggccagca gggggtttgt gtcgcccagg
     5641 agacgctgcg ccagcgtggg aatgccctcg cctaccagat agcggtaatc ttccacgggg
     5701 cgcggcggca gtcccaagag tgccaaacac tcgttcaggg actcagcaat gtcctgtaac
     5761 gaatcgacca acgtgccgtc caggtcgaaa atgcatgctg acggtccgtt cggcgccata
     5821 cgtctcctca gccaaaggct ctgcaaccct gagaccgaat aacagtataa tggcgatagt
     5881 gacctgcgca ttggcgggcg ggtcgccgac cgcaagtgat tggagcagaa gcatgtttct
     5941 tcggacctcg ggcaaactgc tgttggtggt ggcgctggcg gccttggccg gcgtcgtacc
     6001 ggccagatcg caaagcgccg agcgtagcgc caggagcttc gccagcctga tcaacgtcga
     6061 cgctttgatc gacaactatc tgcgctttgt ggcccggaag tacaacctga cggaagagca
     6121 ggatcaatac accgagcaac tggtgcgggc gcgggtcgat gagttcctcg aacggcatta
     6181 cgacgagctg gcagacctgg tggacaagct tgtcatggtg cgcaacggcg gcgatgtggc
     6241 gcccgaagaa ctgatcgagt ggggccggcg cgtccagccg ttatacgtcg aagccaaagg
     6301 cattatcatc caaaccaacg atgaatggcg cggcattctg actgaagaac agcgccgcat
     6361 gcacgacgag gacatccggc tcatgtggga gagcttctct accaccgagg accagctctc
     6421 acgcttgacc gcgggccaaa tgagcgtcga ggaatttcgt aatccgcgca tcgcgcgcca
     6481 ggggcgcgcg caggctgccg cggcccagcc cggcgtcact gcttcggcgg aacgcgccgg
     6541 ttctgcgctc cggcaggcta aagcggccgc tgcggctgac cagggcgtca gccagaagcg
     6601 agccgccgcc aagggggccg ggggcgggcc cgcagcaggg cgctcatcgg ccgcggtgcc
     6661 ggatcgcggt tcaaaacgca ccccgggtaa acgttcggca gcgggtgcaa ccagcgcgga
     6721 tgagtgggag cagtatgtgc aacaattcat ccagcgctac gcgctcacag aggagcaggc
     6781 cagccgcgcg aacaagatcc tgaaaagctg ccagtcgcag cgagaccgct tgatgcgcaa
     6841 ccgcgcggaa cagctcgaaa ggctcaaccg ccaggaagag gccttgcgta cttcatcgga
     6901 caacccgaac aaggccaaag agcaggcgga gataaccgcg cagcgcgcca agctgatggc
     6961 cccgatcgac agcatctttg acaagcagct taagccgcgg ctggagaaaa tacccacgcg
     7021 cgctcagcgc gaagctgccg aaaaggcgcc cgcggccggc cggacagccc cgggcaagcc
     7081 cgcgagtgtc gttcccggca agagcgcagc accgcgggtc gggaagcccg gtgcgccacc
     7141 cacactaccc aaaaagccgg tgccccccgc cccgccgccc gactcggagg gatctcccga
     7201 taatcccgag gaggaagaag actacgagga ggagggggaa aataagtaat aagtagggga
     7261 aacacggtgg tctgactgcc ggccggggtc ggggtgtccc gtacccgccg actggtcagc
     7321 gtcaagtctc ggaacgagcg ggcggcaaaa ctccgccgca cggcagaatc acgcccacag
     7381 aacggccgat agtaggaatt ggagcggacg ccgcgccgaa ctgtgcgggc tgtccgcttc
     7441 aaacaagacc gcacaaggag agagcataat gcgagcttgc ttgaggcgcg gccggatgtg
     7501 gctgctctgt acggcgatcg gcggggtggc agtgttggaa ggttgcgacg cgagcgtacg
     7561 agcggacgtg ctgagcggag tcggatcggc agccaccgga ctggcgacca ccttcattaa
     7621 cgctttcttc accgggttga taaacaagca gtcggatgat gcgcccgttg tgcaaaggtt
     7681 cgatcagtcc gagttacgag ttttttgcta acgccagtat tttgctgacg ccagtgtttg
     7741 ctagacgctg gcttgatgcg tcggggggcg cgccgggctg cacgcgctca tgtggcgaaa
     7801 agctggataa accgcgcgtt aggctcacgc gggtccgggg aggtacgtcg cgccctgcct
     7861 gtgctagatc cgcaagcggc cgcggcgggg aaccatcggc cgttcccgtc ttgatttacc
     7921 cgaattaaga tcccgcgccg aaccacaggt cgtaccatca gtgtgcaatg gacccgttgc
     7981 gtgggtggcg agcgccaggc atgcgcgggc gcggcattgc agcgtgaggt gctccaatgg
     8041 cggcaggcga tttggatgtc ggcgaccgga atgtgcacgg ctatggctct gcaaaggttg
     8101 ccagtacgat caatgtactc gcgggaatat ggctgatcat ttcgcccttc gtgctcagat
     8161 tctggcacgt atcggcggca gcttgggaca acatcatcgt cggcgtcata gtgctcgtgc
     8221 tggccgccat acgggtggcg cagccgacgc gcaatttggc gctgagctgg atcaacctga
     8281 ttttgggcat ctggttgttc atttcgccgt gggtcctgcg cttcagccat gaacgggccg
     8341 cgacgagtaa cgacgtggtg cttggaat
//
LOCUS       DAOSYS010000162         8277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7357_length_8277_cov_4.908173, whole genome shotgun sequence.
ACCESSION   DAOSYS010000162 DAOSYS010000000
VERSION     DAOSYS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7357_length_8277_cov_4.908173"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="wecB"
                     /locus_tag="PLU99_10575"
     CDS             <1..863
                     /gene="wecB"
                     /locus_tag="PLU99_10575"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOO17540.1"
                     /translation="TVMGAALTAFLCGAQVSHVEAGLRTRDKRAPFPEEVNRRVAGVV
                     ADLHFAPTARARDNLLHEGVDPATVFLTGNTVVDALRWMQARVAGQLLPPELDPGDRR
                     LVLVTAHRRESFGEPFRELCLALRQIAERFEDICLVYPVHLNPNVQRPVQEILHGCPR
                     VRLVPPQDYATFVALLSRAHLVLTDSGGIQEEAPALGKPVLVMRDKTERPEAIAAGVV
                     KLVGTQRARIVEEAARLLADDAAYAAMARVVNTYGDGRAAQRIVEVTIDRRMSTPPFQ
                     DQDGPPEDPA"
     gene            860..2497
                     /locus_tag="PLU99_10580"
     CDS             860..2497
                     /locus_tag="PLU99_10580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17541.1"
                     /translation="MTVRVTEPAKGSAVGVLVPAAPPSAARKVRKAPLVWVGLLAAGL
                     WTYHVFSAVMISPDSALFIEYAQRLGAEPIQALRAYDQHPLFPALMLFLRQVCGQFWS
                     EGAAGWILAGRLAGLAGYIGILLAAYALGTRAYDRRIGVITALLAALLPDLSHLAADV
                     LSDTPHLALYLAGLAALATGLESERRARWLLGSAAASALAFLTRPEGGAVLVIGLVFV
                     ALRRDWRLQRRLTLGLSMLAVFAAISAPYQVATGRFVKKKPLPQLLQFGLGDEQAALD
                     LRKPQHSDAAEPRPVAYAGLDRRGALIPINVLYECGRALRVVYAIPVLIGLAFARPRR
                     QPGRILAAAFALHAFLLCALQSSYGYLDRRHTLVLAALAMPLAAAGLVYLAQRIRPQA
                     NFGFQLAAIIGLCVLLTGPWLLRPVNRGHEHVRAAARWFSENTPPDAFLVGNRRLHRV
                     ALCADRAFAEWPWYQGRVPELVEFLASYARPNTYLAIDTLLMTTPPRPATFMEDLHSM
                     LGSQLDLAFSVAAAPGEAPTEIKIYEYRSVPRADGFP"
     gene            2781..7232
                     /locus_tag="PLU99_10585"
     CDS             2781..7232
                     /locus_tag="PLU99_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOO17542.1"
                     /translation="MKARKVYLLQVLLASALAATAWAGVGVSFDGAKYQLALDAGAAF
                     HATPNRVSGERLVAVPNSPAVLVLWPASTDSGTVPFFAISLDGQRVDRVRQANYDIML
                     RAAQFDPGKSVPAVAADLGRGGNVFIVQFVTQILPDFRAQIEELGGRVYQFIPNYAYL
                     VHMNPDTRAAVAALPFVRWVGAYDPGYRIEEVLRDNRANLEELFPFQRYNIMVFESGL
                     KHKHAVAKRIKDIGGTVNMVDAGKFMLHATLTPAQLWQVITWDEVHFVDRWGPYEKDM
                     NNAREYGGANYIETIEGYDGYGVRGEVFDAGFNTSHVDFQSRPLIQHGACGTDSHGAA
                     TSGICFGDGTGDPNARGLLPAGQGIIGDYNTIGLTGPSRYQHTAEQVQAPYYCVFETS
                     SVGSPQVSNYTTDSADTDAMLFDFDILHCQSQSNTGSTLSRPQAWAKNILSGGGIYHY
                     DALDPSSHCWCGGASTGPASDGRIKPDLCAYYDDIRTTTTGSSTAYTDGFGGTSGATP
                     IIAGHAGLFFQMWADGIFGNEVIPGGTVFENRPHMTTAKAVLINNAQQYPFSGGSADL
                     TRIHQGWGWPSLRNIYDRRERMLIIDESEVLQELQSVTYALNVEAGEPEFKATLVYAD
                     PPGSPSAAMARINDLTLRVTDPSGQVYWGNHGLLTGNYSTPGGTANTVDTVENVFVQN
                     PAGGLWVVEVIADEINEDSHPESPEVDADFALVVSGVTPCSSAGTARLGRSLYACEST
                     AEVRVLDCDLNQDPEAIETIAINIASDLEPAGEEVVLTETGVGTAAFRGSITLSATDA
                     PGVLWVADGNVVTITYIDADDGQGHYDVVRTATATVDCLAPEIFDVQASDIEPRSANI
                     SFEANEPVSMIVFYGRSCGALYEQAGITSLSYTPSVALTGLDDDTTYYYAVQATDGAG
                     NFVYDDNGGACYTFTTPEVPDFFTELFSSGNDLDYLSLIFEPNDSNDFYFGCVEAISE
                     LPTDPAGGTAVTLGDDEYVAVNPGATVYLYGVPYTTFYISSNGYITFGSGDSAYSESL
                     ANHFNRPRVSALFDDLRPSSDSTISWRRLDDRVAVTWFHVPEITSGAQNTFQIELFFD
                     GRIRINYLEVGISDGLAGLSEGLGLSPDYLATDLSEMGACYPLNISLPEGAPELLPPG
                     ETTALSVRIRNGAEYYVPDTGMLHYSYDGGPFLTVPLTHVAGDLFEALLPPPSCGDLP
                     AYYFSAAGSLESTVYFPADGPANPLRASVGTLIVLLDDNFQNDLGWTVWNDPTLTTGA
                     WQRAVPLSSGTVGAPFEDYDGSGRCFVTDNRSTSASDYFDVDFGPTILTSPVVDASGA
                     ADLVLKYARWFTCDDTLPPALDYLFSEVSNDGGSTWVRMQRLAPFNGWVYDTLHVADY
                     VPLTAQMQFRFSVADAPNNSTTEAGIDAVLINYVRCDMDAYEVGDLNCDGALNAFDID
                     PFVLALTDPGTYAQAYSDCDYMLADCNEDGAVNAFDIDAFVGLLTGG"
     gene            7661..>8277
                     /locus_tag="PLU99_10590"
     CDS             7661..>8277
                     /locus_tag="PLU99_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO17543.1"
                     /translation="MGDLARLRINEIFHSIQGEGTRAGLPCTFIRTTGCHLRCAWCDT
                     QYAFYEGGWLTLDEILERVRRYDCPLVEVTGGEPLLQPAAVPLLARLCDEFDTVMLET
                     SGAVSIAPVDPRVVRIMDIKCPGSGEAERNCWENLELLTARDELKFVLLNRADYDWAR
                     EVLQRYDLSKRCPVLFAPVFGQLTPRELATWILADRLNVRLGLQL"
BASE COUNT         1443 a         2562 c         2698 g         1574 t
ORIGIN      
        1 ctacggttat gggcgctgcg ttaacggcct ttctgtgcgg cgcacaggtg tcgcacgtgg
       61 aggcgggcct gcgaacgcgt gacaagcggg ccccgtttcc ggaagaggtg aaccggcgtg
      121 tcgcgggcgt ggtggccgac ctgcacttcg ccccaacggc gcgagcgcgc gacaacctgc
      181 tgcacgaagg agtcgaccca gccacggttt ttctgacggg taacaccgtg gtggacgcgc
      241 tgcgctggat gcaggcgcgc gtcgccggac agcttttgcc gccggaactc gatccgggcg
      301 atcggcggct ggtgctggtg acggcccatc ggcgcgaaag cttcggcgag ccgtttcgcg
      361 agttatgcct ggcgctgcga cagatcgcgg aacgcttcga ggacatatgc ttggtttacc
      421 cggttcacct gaatccgaac gtgcagcggc ccgttcagga gattctccac ggctgcccgc
      481 gagtgcggct tgtgccgcca caggactatg ccacattcgt cgccttgctg tcgcgggcgc
      541 acctggtgct gacggattcg ggcggcatcc aggaggaggc cccggccctg ggcaagccgg
      601 tgttggtaat gcgcgataag accgaaaggc cggaggctat tgcggccggc gtggtcaagc
      661 tggtgggcac gcaacgtgcg cgaatcgtcg aagaggccgc gcgactgctg gccgacgatg
      721 cggcctacgc ggcgatggcg cgcgtggtga atacctacgg ggacggccgg gctgcgcagc
      781 ggattgttga agtcacgatc gacaggcgga tgagcacgcc gccgtttcaa gaccaggacg
      841 ggccgccgga agatccagca tgaccgttcg agtgaccgag ccggcgaaag gaagcgcggt
      901 tggggtgctt gtccccgcag cgccgccgtc cgcggcgcgc aaagttcgca aggctccgtt
      961 ggtgtgggtg ggtctgctgg ccgcgggttt gtggacgtat cacgtcttct ccgcagtcat
     1021 gatctcgccc gatagcgcgc tattcatcga atatgcacaa cggctgggag ccgagcccat
     1081 ccaggcgctg cgggcctacg accagcatcc gctgtttccc gcccttatgc ttttcctgcg
     1141 gcaggtttgt ggacagttct ggagcgaagg tgcggcgggc tggattctgg caggccgcct
     1201 ggcgggcctg gcgggatata tcggaattct gctggcggcc tatgcgctgg gaacgcgggc
     1261 ctacgaccgc cgaatcgggg taataaccgc cttgctggcc gccctgcttc cagacctttc
     1321 gcaccttgcg gccgatgtgc tgagcgacac gccgcacctg gcgctatacc tggcgggatt
     1381 ggccgcgctg gcgacggggt tggagtcgga gcggcgcgca cgctggctgc tcggatcggc
     1441 cgcggcatcc gctctggcct ttttgacgcg gccggaaggc ggggcggtgc tggtgatcgg
     1501 actggtgttc gtggcactgc ggcgtgactg gcgtctgcag cggcgtttga cgctgggcct
     1561 gtcaatgctg gccgtcttcg ccgcgatcag tgcgccatac caggtcgcga ctggaaggtt
     1621 cgtcaagaaa aaacccttgc cacagttgtt gcaattcgga ttgggtgacg agcaggcggc
     1681 gctggacttg agaaagccgc aacactctga cgcggccgaa ccgcgaccgg ttgcctatgc
     1741 cggcctggat cgtcgcgggg cgctcattcc aatcaacgtc ctatatgagt gtggccgcgc
     1801 cctacgcgtg gtgtacgcca tcccggtcct cattgggctg gcctttgcgc gacccagacg
     1861 acaaccgggt cgcatcctgg cagcggcctt tgctctacac gcgtttttgt tgtgcgctct
     1921 gcaatcgagt tatggctatc tggaccggcg gcatacgctg gtgttggcag cactggccat
     1981 gccgctggcc gcggcgggac tggtgtacct ggcgcagcgg attcggccac aagcgaactt
     2041 cggcttccag ttggcggcca taatcggcct gtgtgttttg ctgactgggc cgtggctgct
     2101 ccggccggtc aatcgcgggc atgagcacgt gcgtgcggcg gcccgttggt tctcggagaa
     2161 cactccaccg gacgcctttc ttgtgggcaa ccgccgactg caccgcgtcg cattgtgcgc
     2221 cgaccgcgcc ttcgccgagt ggccatggta ccagggacgc gtgccggaat tggtggaatt
     2281 cctggcatca tacgcgcgcc cgaatacgta cctggcgatc gatacgctgc tgatgaccac
     2341 gccgccgcgc ccggcgacat tcatggaaga ccttcactcc atgctgggat cgcaattgga
     2401 cctggccttc agcgtagcgg ccgcgcccgg ggaagcgccg accgagatca agatttacga
     2461 gtatcgcagc gtgccgcgcg ctgacggttt cccgtaggcg gtgatattcg gacgctaaag
     2521 acacggtgga cctgccggac ccgcagaagc gattgctgaa cggccggaac gcgcggcgca
     2581 aggggaactg cctcgtggaa gattggcttt gtccacgggg gggcgtccgg tatgatgaat
     2641 gagctcgggc aagcgctgga ggcaagccct tgaacggaag ggaagaaggt tcaacccctc
     2701 gggctacgag cgcgcgccaa atttcgtgtt tgtcttggag gcgcggtgaa cgggcccgcc
     2761 ggattcgaaa ggagcatgag gtgaaagcga ggaaggtgta tcttctccag gtactgttgg
     2821 cgagcgcgct ggccgcgaca gcctgggccg gggtgggtgt cagctttgac ggggccaagt
     2881 accagttggc ccttgacgcc ggcgcggcat ttcacgccac gcccaatcgc gtcagcggcg
     2941 agcgcctggt ggcggtcccg aattcaccgg ccgttttggt gctgtggccc gcgagcacgg
     3001 actccggcac agttccgttc ttcgctatca gtctcgatgg tcagcgcgtc gaccgcgtac
     3061 gccaggcgaa ctacgacatc atgctgcgcg cggcgcagtt cgatcccggc aaatcggtgc
     3121 cggccgtggc ggcggacctc ggccgcggcg gaaacgtctt catcgttcag tttgtcacgc
     3181 agattctgcc ggacttcagg gcacagattg aggaactggg cggccgggtg taccagttca
     3241 ttccgaacta tgcctacctc gttcacatga atccggacac acgggcggcg gtggcggcgc
     3301 tgccgtttgt acgctgggtg ggcgcctacg atcccggtta ccggatcgag gaagtgctgc
     3361 gcgacaaccg cgcgaatctc gaggagttgt tcccgttcca gcggtacaac atcatggtct
     3421 tcgagtccgg gctaaaacac aagcacgcgg tggccaagcg catcaaagac atcggcggga
     3481 ccgtgaacat ggtcgatgcc ggcaagttca tgctgcatgc cactctcacc cccgcgcaac
     3541 tctggcaggt aatcacctgg gacgaagtgc acttcgtgga tcgctggggc ccgtacgaga
     3601 aggacatgaa caacgcccgc gagtacggcg gggcgaacta catcgaaacc atcgagggct
     3661 atgacggcta cggcgttcgc ggcgaagtct tcgacgccgg gttcaacacg tcgcacgtcg
     3721 atttccagtc gcggcccctc attcagcatg gcgcttgcgg cacagattca cacggcgccg
     3781 cgacgtccgg catctgtttc ggcgacggga ccggcgatcc gaacgcacgc ggcctgctgc
     3841 cggccggaca gggcatcatc ggcgactata acaccatcgg cctgaccggc ccgagccggt
     3901 atcagcatac ggccgaacaa gtgcaggccc cgtactattg cgtgttcgaa acgtccagcg
     3961 tcggcagtcc gcaggtttcc aactacacaa ctgattccgc cgataccgac gcgatgctgt
     4021 tcgatttcga cattttgcat tgccagtctc agagcaatac gggcagcacg ctgtcgcgcc
     4081 cgcaagcctg ggcgaagaac atcctgtctg gcggcggtat ttaccactac gacgcactgg
     4141 acccgagctc ccactgctgg tgtggcggcg ccagcaccgg gccggcatct gacgggcgta
     4201 tcaaacccga tctgtgcgcg tattacgacg acattcgcac gacgactacg ggcagctcga
     4261 ccgcctacac cgacggtttt ggcggcacca gcggcgccac cccaatcatt gccggccacg
     4321 ccggtctgtt cttccagatg tgggccgacg gcattttcgg aaacgaggtc attccgggcg
     4381 ggaccgtatt tgaaaaccgc ccgcacatga cgactgccaa ggccgtgctg atcaacaacg
     4441 cccagcagta tccattctcc ggcggcagcg ctgacctgac gcgcatccac cagggctggg
     4501 gctggccgag cctgcggaac atctatgacc gccgcgagcg catgttgatc attgacgagt
     4561 cggaggtgct ccaggagctg cagagcgtga cctacgccct gaacgtcgag gccggtgagc
     4621 cggaattcaa ggccacgctg gtctacgccg acccgccggg ctcgccgtcg gccgccatgg
     4681 cccgcatcaa cgacctgacg ctgcgcgtga ccgatcccag cggccaggtc tactggggca
     4741 accacggtct gctgaccggg aactactcca cgccgggcgg gacggccaac accgtagaca
     4801 cggtggagaa cgtattcgtg cagaacccgg ccggcggctt gtgggtggtc gaggtaattg
     4861 ccgacgagat caatgaagac agccatcccg aatcgcccga agtcgacgcc gatttcgcgc
     4921 tggtcgtaag cggcgtaacg ccctgctcgt cggccggcac cgcgcgcttg ggccgctcgt
     4981 tgtacgcctg cgagagcacg gcggaagtgc gcgtgctcga ctgtgatctg aaccaggatc
     5041 cggaggcgat tgaaaccata gcaatcaaca ttgcctccga cttggagccg gccggtgagg
     5101 aagtggtcct gacggagacc ggagtaggga cggcggcctt ccgcggttca attacgctga
     5161 gcgcgacgga cgcgccgggc gtgctgtggg tagccgacgg caacgtcgtg acgatcacgt
     5221 atatcgatgc ggatgacggg cagggccact acgacgtggt gcggactgcc acggccaccg
     5281 tcgattgcct ggcgccggag attttcgacg ttcaagccag cgacatcgag ccgcgttcgg
     5341 cgaatatctc gttcgaggcg aatgagccgg tatcgatgat cgtgttctat gggcggtcgt
     5401 gcggcgcgct ctacgagcag gccggcatca cgtcgctctc gtacacgcca tcggtggcgt
     5461 tgaccgggct ggatgacgac accacttact attatgcggt tcaggccaca gatggcgccg
     5521 gtaacttcgt ctacgacgac aacggcggcg cctgctacac gtttactacg cccgaagtgc
     5581 cggatttctt caccgagctc ttctcgtccg gcaatgacct ggactacctg agtttgatat
     5641 tcgaacccaa tgattcgaac gacttctact tcggctgcgt cgaggccatc tccgagctgc
     5701 cgaccgatcc ggccggcggc acggcggtca cgttgggtga cgacgaatac gtggctgtga
     5761 acccaggtgc aactgtctac ctgtatggtg tgccctacac gaccttctac atcagcagca
     5821 acggctacat cacgtttggt tccggcgaca gcgcctacag cgaatcattg gccaatcatt
     5881 tcaaccggcc gcgcgtgtcg gccctgttcg atgacctgcg cccgtcctcc gacagtacga
     5941 taagctggcg ccggctggac gatcgcgtcg cggttacctg gttccacgtt cccgagatca
     6001 cttcgggcgc acagaatacg ttccagatag agttgttctt cgacggccga ataagaatca
     6061 attatctgga ggtcggtatc agtgacggtc tggccgggct atcagaaggt ctgggcctgt
     6121 cgcccgatta cctcgccacg gatttgtcgg agatgggcgc ttgctatccc ttgaacattt
     6181 cactgcccga aggcgcgccc gaactgctgc cgccgggtga aacgacggcg ctcagtgtcc
     6241 gcattcgcaa cggggctgag tactatgttc ccgatacagg gatgctgcac tacagttacg
     6301 atggcgggcc cttcctgact gtgccgctga cgcacgtggc gggcgatctc ttcgaggccc
     6361 tcctgccgcc gccgagctgc ggcgatttgc cggcctatta cttcagcgcg gccggcagcc
     6421 ttgagtcgac tgtgtacttc ccggcggacg gccctgccaa cccactgcgg gccagcgtgg
     6481 gaacgctcat tgtgctgctt gacgacaact tccagaatga tctgggatgg acagtgtgga
     6541 atgatccgac cctgaccaca ggcgcgtggc aacgggccgt cccgctgtcc agcggcacag
     6601 tcggcgcgcc atttgaggac tacgacggct ctggccgctg ctttgttact gataatcgct
     6661 ccacatcggc cagcgactat ttcgacgtcg actttggtcc tactatcctg acttcgcccg
     6721 tcgtcgacgc cagcggcgcc gcggatttgg tgctgaagta cgcgcgctgg ttcacgtgcg
     6781 acgacacgct cccgccggct ttggattatt tgttcagcga ggtctctaac gacggcggca
     6841 gcacctgggt gcgcatgcaa cgcctcgcgc cgttcaacgg ctgggtgtac gacacgctgc
     6901 acgtcgcgga ttacgtgccg ttgacggccc agatgcagtt ccgcttcagc gtcgcggacg
     6961 cgccgaacaa ctcgaccacg gaagccggca tcgacgccgt actaatcaat tacgtgcgtt
     7021 gcgatatgga cgcctacgaa gtcggcgact tgaactgcga cggcgcgctg aatgcgtttg
     7081 acatcgaccc attcgtgctg gcgctcaccg accccggcac gtatgcgcaa gcctatagcg
     7141 actgcgacta catgctggct gactgcaacg aagacggcgc cgtcaatgcg ttcgacatcg
     7201 acgcgttcgt ggggctgctg accggcggct gatggccagg cggtgaaatt gagaattagg
     7261 tagatcaagg ggcggcgcgc tgtgcgtggc gcgccgcccc ctttcttgag gccgggctta
     7321 gccgacgccg tttgacatcg ttggcgcaca ggaagtcaca agtctatcat ccgccaggcg
     7381 ttgaacgcgc cgcccgacgg cgctagtatc aaaagccggc acgtgttacc ccccggcacg
     7441 tccggccggg cgtcggttgc cagcgcgcac ccagacgcct gtttcttatc gccggccgag
     7501 gggtaccggt tttgccctgt cgttaggccg gcaagtagcg cgattctgcc ttacgattct
     7561 gggcgtgatc cggtcttgcc gccctgcggc ttcaagccgg cacaggcgtt acaatctgca
     7621 ctggcagtta atcgacccgg aaagggcgtt cgcagcaata atgggcgatt tggcgcgttt
     7681 gcgcatcaac gagattttcc actcaatcca gggtgagggc acccgggccg gcttgccctg
     7741 caccttcatc cgtacgaccg gttgccacct gcgctgtgcg tggtgcgaca cgcagtacgc
     7801 cttttatgaa ggtggctggc tgacgctgga cgaaatcctc gagcgcgtgc ggcgttatga
     7861 ctgcccgctg gtcgaggtga ccggcggaga gccacttctg caaccggccg cggtgcctct
     7921 gctggcgcgg ctctgcgacg agttcgacac cgtcatgctc gagacgtcag gggcggtttc
     7981 gatcgccccg gtggatccgc gtgtcgtacg catcatggat atcaagtgcc ccggcagcgg
     8041 cgaggcggag cgcaattgct gggaaaatct cgagctactg accgcgcgcg acgagctcaa
     8101 attcgtgctg ctgaaccggg ccgactacga ctgggcccgc gaagttctcc agcgctacga
     8161 cctgagcaag cgctgcccgg tgctctttgc cccggttttc ggccaactca ccccgcgcga
     8221 gctggcgacg tggattctcg cggaccgcct gaacgtgcgg ctcgggttgc agttgca
//
LOCUS       DAOSYS010000163         8160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7487_length_8160_cov_5.504750, whole genome shotgun sequence.
ACCESSION   DAOSYS010000163 DAOSYS010000000
VERSION     DAOSYS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7487_length_8160_cov_5.504750"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PLU99_10595"
     CDS             <1..469
                     /locus_tag="PLU99_10595"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369275.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOO17544.1"
                     /translation="SAIGVTVERVSAEKVNVSSARRLEPIEVTTQPFPGFPTDLQAQM
                     MALLCLADGNSIITEKIFPDRFLHVAELARMGALLHKEGPTVIVEGVKRLIGAPVMAS
                     DLRASAALVLAGLCARGVTRVNRVYHLERGYSKLDEKLRNLGAEIERREGDDL"
     gene            complement(819..1850)
                     /locus_tag="PLU99_10600"
     CDS             complement(819..1850)
                     /locus_tag="PLU99_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO17545.1"
                     /translation="MASRSEWGDAGFSRHCAPFKLHAAKRPGGTRHARSRGASARAAY
                     GLGFTLVELLVVIAVLSMLMSILLPATSAARRAARTSVCSSTLRELARGWTIYGQEHA
                     GTLVAGRAAKENGTNNLYWVGNGWKYRPRWHITLGASVVIYAFNQPSTQDEHQRFDNR
                     LLVCPEAPGWVSEKNTSYGYNFQFLGNARKISGTERFINFPVNDSHIVGDTVVLADSL
                     GTAASFPAVERMPNCPDGTTGDVTTDVRTLGYHGFMLDPPRLTPTSDRCDDKNRDYRS
                     GPDARHDGKANFAFADGHVRLAGPEALGYGILPDGSYEFGYEFDKTLVTNRRFSGTGR
                     DDDPPSIVP"
     gene            2045..2362
                     /locus_tag="PLU99_10605"
     CDS             2045..2362
                     /locus_tag="PLU99_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009416926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17546.1"
                     /translation="MTRPKTIMVDLDGVICTEERTFERPLAQPMPGAREALARLKAAG
                     HTVIVYTGRNWPEYRVTKKWLDDHGFVYDALEMGKPIADVWIDDRAIRFSDWDSTLTQ
                     LGV"
     gene            2930..3712
                     /locus_tag="PLU99_10610"
     CDS             2930..3712
                     /locus_tag="PLU99_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17547.1"
                     /translation="MLRIVSVLSLSMAIVWLSGCACCPKKDQCPTTAMCPAPCPEDIE
                     ASALPPNAKPGECYAKVYIPPQYETYTERVCVREASEQLEVVPAQYEWVEERICVKDA
                     CEELRVTPAQFACREEKVLVQPSYTEWQVNKQPQCVAFPGQPAQDVFCLVQHPCVEKT
                     IARTVMERPPCAEKVTIPAEYQTVRRQKLVCPATTRKVTIPAEFADVQKTRKVCDAKV
                     VWRRVPCDVPTGGVVSLPGEQCPPFPLTPPVPVGSQTITPHR"
     gene            complement(3874..4848)
                     /locus_tag="PLU99_10615"
     CDS             complement(3874..4848)
                     /locus_tag="PLU99_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17548.1"
                     /translation="MRGATLCARRVKALFGSLRSKLGKVSLPSPSDPVTQCILGILSR
                     DVPEPKAREALDRIRGMVVDFNELRVVPPIELAQMLGDYPDVRLKCEDISRALNKIFA
                     LHHNVTLDHLLGMPKKEVIIYLSGLDGLEDYTRARIRLLALQQHAVPLDEAMWAYARQ
                     QEIIDPRCQLGEAQSFLERQVSEGDALEFYVLLNRQAWAEMGTAVRKRQVARILSVPP
                     DRTTRNMLQAIGAGAEVDEPVLVDEDLELEPLADEAPPPPEPVKPAAAETPPRKRRPQ
                     RPAPARPAKQKPPAAKRKAPAAKAAPSKAPERRKPAKAPRAKAAKAKR"
     gene            complement(4852..5349)
                     /gene="rbfA"
                     /locus_tag="PLU99_10620"
     CDS             complement(4852..5349)
                     /gene="rbfA"
                     /locus_tag="PLU99_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOO17549.1"
                     /translation="MSRRTERIGSVIRNIVSEAIQTRLADPRLEPLTSITRVDVSADL
                     SLARVYVSVMAKPAGQKLAVAALANAAGRLRGAVADQVHMRQVPRLEFVLDDSLKQAF
                     ETVQAIDSAMAELGEKPPWESAAEAEPADEEEPAGAGDESTAARPADDANAPVQPAQP
                     GEEDR"
     gene            complement(5431..5712)
                     /locus_tag="PLU99_10625"
     CDS             complement(5431..5712)
                     /locus_tag="PLU99_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOO17550.1"
                     /translation="MVVGVLRIRLGVFEALSLKDKRRVVKGLKDRLAARHNISIAEVD
                     DLDHRQAATLAMAMVANQARYVEGALSKIVDEVRATSSASLLDYDIELL"
     gene            complement(5726..5893)
                     /locus_tag="PLU99_10630"
     CDS             complement(5726..5893)
                     /locus_tag="PLU99_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17551.1"
                     /translation="MHYSSDPAASDLGGAEVCGVFDRGEPRKVRAGRKWGIKLGGVRE
                     GYEAVQAAPEL"
     gene            complement(5916..7775)
                     /gene="infB"
                     /locus_tag="PLU99_10635"
     CDS             complement(5916..7775)
                     /gene="infB"
                     /locus_tag="PLU99_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOO17552.1"
                     /translation="MASATGMGLRERRAAERRRAAAAAAGVSAARKAPIELTAPIALK
                     EFCSAVGIPFSTVSQKLLEHTNRLWRINENIDADTLELLTMELGVPVTIAKQKTALER
                     LKEQFDARPREHLKPRPPVVAMLGHVDHGKTSLLDAIRKADVAAGEAGGITQHIGAYR
                     VERGDWRVTFLDTPGHEAFTAMRARGANLTDVVVLVVAADDGVMPQTIEAINHARAAN
                     VQIVVALNKIDLPNADVNRVYSQLAAQELIPSEWGGQTDVIKTSATTGQGVDDLIAHL
                     STLSDLMELRADPTVPASATVIESQMKEGQGVAAKVLVREGTLRTGQIVVCGGGYGRI
                     RMLRDDRGRRIKEALPGWPVEVSGLDELPQAADQMYQVDDLKQAKDIAEEMRQRRREA
                     ELEGLRKPRTLEDLLAAGDESQIPELNLIIKADVQGSVDVLKKAVSELPREKAQSRVL
                     HAAVGAISEADVQLARASNALIIGFHVVAEERARQLAEELGVQVRLYRVIYEMLDDVH
                     KALAGLLEPVQRQEVRGAAEVRKIFNITRVGLVAGCRVTSGVMHRHHLVRVIRDGRVV
                     ADNRAIGSLRHVKDDVREVRAGLECGIKLEGFDDIKPGDVLEAYEVVQVAQEL"
     gene            7806..>8160
                     /locus_tag="PLU99_10640"
     CDS             7806..>8160
                     /locus_tag="PLU99_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17553.1"
                     /translation="MPLFQAFAHIDRAAVTARTARPDSCPVTLIRLPMPSSATLAAGC
                     GAGLGNDVDRRSTSGRRLTRARWANGLWRLDSDDARALQFGRARHYLWAGWLYRRRRR
                     RRREYRWRGAGRRLGR"
BASE COUNT         1388 a         2646 c         2565 g         1561 t
ORIGIN      
        1 cagcgcgatc ggcgtcacgg tcgagcgcgt cagcgcggag aaggtgaacg tctcgtcagc
       61 ccggcggttg gagccaatcg aggtgaccac gcagccgttc cccggcttcc ccaccgattt
      121 gcaggcgcag atgatggccc tgctttgcct ggcggacggc aacagcatca ttacagagaa
      181 gatattcccg gaccggtttc tgcacgtggc cgagctggcc cgcatgggtg cgctcctgca
      241 caaggaaggg cccacggtca tcgtcgaggg agttaaacgc ctgatcggcg cgccggtcat
      301 ggcctccgac ctgcgtgcct cggccgccct ggttctggcc ggcctgtgcg cccgcggcgt
      361 cacgcgagtc aaccgggttt accacctgga acgcggttac tccaagctgg atgagaagct
      421 gcgcaattta ggcgctgaaa tcgagcgcag ggaaggcgac gacctttaag tgcggagccg
      481 gctttcgcgg cggcaagcct ttgaactgtt gaggccgggg ttgcagacaa ggggtgtcga
      541 gtccagcgtt gcaccgaagc aggcgccgtg agcggtttcg acgccaattc gggcgtgatg
      601 ctccgcacag ggccgtcact cgattgtaag gatgtgtaga aacggcgcgc ccccgcaaca
      661 aatctctgca acaaacgttc gggaacttcg tataaatcag tgaagagacc ctcctactcc
      721 ctcctacccc tccagcgcgt tgggccgccg caaaacggcg gccctttttt tttcgcgcga
      781 aactcggcgg caacgggcgg gggaatcccg gcgcttcttc acggcacgat gctggggggg
      841 tcatcatcgc gccccgttcc gctgaagcgg cggttcgtca ccagcgtttt gtcgaattcg
      901 tatccgaatt cgtagctgcc atccggaagt atgccgtaac cgagcgcctc cgggccggcc
      961 aaccggacat gtccgtcggc gaacgcgaaa ttcgccttgc cgtcgtgccg ggcgtcaggc
     1021 ccgctgcggt agtcacggtt cttgtcgtca catctatcgc tggtcggcgt caggcgcggc
     1081 gggtcaagca tgaagccgtg ataccccagc gtgcgcacgt cagtagtaac gtcgcccgtg
     1141 gtgccgtccg ggcaattcgg catccgttcc accgccggaa aagaagccgc ggtgcccagc
     1201 gagtccgcca ggactaccgt atcgcccacg atatggctgt cgttgaccgg aaaattgatg
     1261 aagcgctccg tgccggatat cttgcgggcg ttcccgagaa actggaaatt gtagccgtac
     1321 gaggtgttct tctcgctgac ccacccgggc gcctcggggc agaccaatag gcggttatca
     1381 aagcgctgat gttcgtcttg cgtggaaggc tggttgaaag cgtaaatgac aaccgacgcg
     1441 cccagggtga tgtgccagcg cggccggtat ttccagccgt tgcccaccca gtacaagttg
     1501 ttggtgccgt tctcctttgc ggcccggccg gcgaccagtg tgccggcgtg ctcctggccg
     1561 tatatggtcc agccgcgcgc cagttcgcgc agggtgctgc tgcaaacgct ggttcgcgcc
     1621 gcgcggcgtg cggcgctggt cgccggcaac aggatggaca tcagcatgct gagcacggcg
     1681 atcacgacca gcagctcgac tagcgtgaag cctaagccgt atgccgccct cgcgcttgcg
     1741 cctcggctgc gggcgtgccg cgtgccgcca ggtcgcttcg ccgcgtgtaa cttaaaaggc
     1801 gcacagtgcc tggaaaatcc cgcgtcgccc cactcactcc ggctcgccat cgggttctcc
     1861 atcgtttctg aacgtcacga caaagagact aatcgcggaa cgctttgctt tataggacaa
     1921 aaacgcttta taggacaggg ccggtttgcg gcgacttgga gcctatggcg gattaccaac
     1981 gcatctgtca gactgtagtg ggcaatttgg tccaccagcc gatttgacga caaggagcac
     2041 tcgcatgacc cgccccaaaa cgatcatggt cgaccttgac ggcgtgatct gcaccgagga
     2101 acgcacattc gagcggccct tggcccagcc gatgccgggc gcccgcgagg ctctggcgcg
     2161 gctgaaggcc gccgggcaca cggttatcgt gtatacgggc cgcaactggc cggaataccg
     2221 ggtcacgaag aaatggctcg atgatcacgg gttcgtttac gacgcactcg agatgggcaa
     2281 gcccatcgcg gacgtttgga tcgacgatcg cgcgatccgc ttctccgatt gggacagcac
     2341 gctgacgcaa cttggggtat gaagctggcg gcggcgcacc gcaggcaggc cggtcgcggt
     2401 gccaggtcgc ggctggccgg gtcccgctcc cgagttcgaa agctcctgca gcgctcgaac
     2461 gggccgcttc ttgacgtaac cggcgtgtat ggcgtgggtc gacgttttgg cgcgcgggac
     2521 ttacggggtg cgttttctct ggccactttc agcccggacc ggctatcgcg cgcgtaagca
     2581 cgcgttttga cattggcgta agcaacgcag tgctcgaatg tcatcccaag cccgataaga
     2641 ccgttaccag atcgacgtag cagcctagtc ttcgtggcag atgtccccgt cattacattg
     2701 gtgtaggcct gcattaatct cgcggggcac acgcggcagg cgcgccgaaa atgcggcctg
     2761 tatgtcagga ctgaattctt acgccgctct cactcagaat tcagttgtaa ttcatagtct
     2821 ggctatacag taatctttgg cgtcaccccc agggaacgag acgcacagta ttggtcccgt
     2881 aggttctgca tttggcccag tcccaagggc ctggaaggaa ggaagtctca tgttaaggat
     2941 cgtgtctgta ctgtcattgt ctatggcgat tgtgtggcta agcggctgcg cctgctgccc
     3001 caaaaaggat cagtgtccca caacggccat gtgcccggcg ccctgccccg aggacatcga
     3061 agcgtccgcc ttgccgccca atgccaaacc cggcgagtgc tatgccaagg tttacatccc
     3121 gccgcagtat gaaacctaca ccgaacgggt atgtgtgcgc gaggccagtg agcagctcga
     3181 ggtggtgccg gcgcagtatg agtgggtcga agagcgcatc tgcgtgaaag acgcctgtga
     3241 ggagctccgc gtgacgcccg ctcagttcgc gtgccgcgag gaaaaggtgc tcgtccagcc
     3301 cagctacacc gagtggcaag tcaataagca gccgcagtgc gtcgcgttcc cgggccagcc
     3361 ggcgcaggac gtcttctgtc tagtgcaaca tccctgtgtt gagaagacaa ttgccagaac
     3421 ggtcatggaa aggccgccgt gcgccgagaa ggtgaccata ccggctgagt accagaccgt
     3481 ccgccggcag aagttggtat gcccggccac gacgcggaaa gttaccatcc ccgccgagtt
     3541 cgcggacgtt cagaagacgc ggaaggtctg cgacgccaaa gtagtgtggc ggcgcgtgcc
     3601 ctgtgatgta cctaccggtg gtgtggtcag tctgcccggc gaacagtgtc cgccgttccc
     3661 cctcacgccg cctgttcccg taggttctca gaccataact ccgcatcgct aggctgaagg
     3721 ccacaactgg ttggattaat agcattcgcc aggctgggac ttcgccgcag caatgacgca
     3781 gccgtccagg gagtccggag acgggctccc tggtgcggcg ttattagcga atgtcatccg
     3841 gcggagactg ccagccccct cgggcattcc aggtcaacgt ttggctttgg cagccttggc
     3901 gcgcggggct ttggcgggct tgcggcgctc tggggctttt gacggggcgg ccttggccgc
     3961 cggcgccttg cgcttcgcgg cgggcggctt ttgtttggcc ggtctggcgg gggccggacg
     4021 ctgcggccgg cgtttgcgcg gcggggtctc cgcggcggcc ggtttgaccg gctcgggcgg
     4081 aggcggggcc tcgtcggcca gcggctcgag ctcaagatct tcgtcaacga ggacgggctc
     4141 atcgacctcc gcgccggcgc caatggcttg gagcatgttg cgcgtcgtgc ggtcaggcgg
     4201 cacgcttaga attcgggcca cctggcgttt ccgcacggcc gtccccatct cggcccaggc
     4261 ttgccgatta agcagaacat agaattccaa ggcgtcgcct tcgcttacct ggcgttccag
     4321 aaaggactgg gcctcgccga gttggcagcg ggggtcgatg atttcttgct gccgcgcgta
     4381 ggcccacatg gcctcgtcga gtggaacggc gtgttgttgc aaggcgagta ggcggatgcg
     4441 ggcccgggtg tagtcctcca gtccgtcgag gccgctcagg tagatgatca cctctttctt
     4501 gggcattccc aaaaggtggt cgagcgtcac gttgtggtgc agggcgaaaa tcttgttcag
     4561 ggcgcgggaa atgtcctcgc atttgaggcg cacgtccggg tagtcgccca gcatctgcgc
     4621 caactcgatc ggcgggacca cgcgcagctc attgaaatcc acgaccatcc cgcggatgcg
     4681 atccaacgcc tcacgcgcct tcggctccgg gacatcacgc gagagtatcc caagtatgca
     4741 ctgcgtcacc gggtcgctgg gactcggcaa cgaaaccttg cccagctttg aacgcagtga
     4801 gccgaaaagg gccttaacac gccgcgcaca tagggtagcg cccctcatgg atcagcggtc
     4861 ctcctctcca ggctgagccg gctgcaccgg cgcgttcgcg tcatccgcgg gccgggccgc
     4921 cgtggactcg tcccccgccc ctgccggctc ctcttcgtcc gcgggctcgg cttccgccgc
     4981 gctttcccac ggcggtttct cgccgagttc cgccatcgct gaatcgatgg cctgaaccgt
     5041 ctcgaaggcc tgcttgagcg agtcgtccag cacgaactcc agccgcggaa cctggcgcat
     5101 gtggacctgg tccgcgacgg cgccgcgcag acgcccggca gcgttggcca gcgccgccac
     5161 cgccaacttc tggccggccg gcttggccat cacgctgacg taaacgcggg ccagggatag
     5221 atcggcgctg acatcgacgc gggttatcga ggtcaacggc tccaggcgcg gatccgccag
     5281 ccgggtctga atcgcctcag acacgatatt ccgaatgaca ctgccgatgc gttcggtacg
     5341 gcgggacatc gtaagttttt ggttctcagt tctcagttgt cagtcagtgt caattggtcg
     5401 gcctctctaa caccgggcaa ctgaagactt ctaaagcagc tcgatatcgt aatccagcaa
     5461 agacgcactc gaggtagctc gcacttcatc cacaattttg ctcagggcac cttccacata
     5521 gcgggcttgg ttggcaacca tggccatagc cagcgtggcc gcctggcggt gatccaggtc
     5581 gtcgacttcg gctatggaga tgttatgccg ggctgccagc cgatctttga ggcccttcac
     5641 cacgcgccgc ttgtccttca aggaaagggc ctcgaaaacg ccgagcctga tccgtagtac
     5701 accaaccacc atccgccaat gtcactcaaa gttccggcgc cgcctgcacg gcttcatacc
     5761 cctcgcgtac gccgcccagc ttgatgcccc acttccggcc ggcccgcacc ttccgaggct
     5821 cgcccctatc gaagacccca cagacttccg cccctccaag gtccgaagct gccgggtccg
     5881 acgagtaatg catggatacc gtccggtacg cgacgttaga gttcctgggc gacctgtacg
     5941 acttcgtatg cttcgagtac gtcgcccggc ttgatgtcgt cgaagccttc cagcttgatg
     6001 ccgcattcca gcccggcccg cacctcgcgc acgtcgtcct taacatgccg caacgaccca
     6061 atggcgcggt tgtcggccac gacccgccca tcccgaatga cgcgcaccaa gtggtggcga
     6121 tgcatgacgc cgctggtcac gcggcagcct gccaccagcc ccacgcgcgt aatgttgaag
     6181 atcttgcgga cttccgccgc tccgcgcacc tcctgccgct gaaccggctc gagcaggccg
     6241 gccagcgcct tgtgaacgtc gtccagcatc tcgtatatca cgcggtacag ccggacctgc
     6301 acgcccaact cttccgcgag ttggcgggcc cgttcctctg ccaccacgtg aaaaccgatg
     6361 atgagcgcgt tcgaggcacg cgccagttgc acgtcggcct cgctgatggc gcccacggct
     6421 gcgtgcagca cgcgtgattg ggccttctca cggggcagct cgctgaccgc cttcttgagc
     6481 acgtccacgg agccctgaac gtcggccttg atgatcaggt tcagctccgg tatctgggat
     6541 tcgtcgccgg ccgccaacag gtcttccagc gtccgcggct tgcgcaggcc ttcaagctcg
     6601 gcctcacgcc gccgctgccg catctcctcc gcgatgtcct tggcctgctt gaggtcatcg
     6661 acctggtaca tctggtcggc cgcttggggc aactcgtcca ggccgctcac ctcgaccggc
     6721 cagccgggca aggcttcctt tatccttcgg cccctgtcgt cgcggagcat gcggatgcgg
     6781 ccgtagccgc cgccgcagac cactatctga cccgtgcgca acgtgccttc gcggaccagc
     6841 accttggccg ctacgccctg gccctccttc atctgggact cgatcacggt ggcgctggcg
     6901 ggcacggtcg ggtcggcccg gagttccatg aggtcgctca acgtgctcaa atgcgcgatc
     6961 aagtcgtcga cgccctggcc ggtggtggcg ctggttttga tcacgtccgt ttggccaccc
     7021 cattcggaag gaatcagctc ctgcgccgca agctgggagt aaacgcgatt cacgtcggcg
     7081 ttaggtaggt cgatcttgtt cagggctacc acgatctgaa cattcgcggc gcgggcgtgg
     7141 ttaatcgcct caatcgtctg cggcatgacc ccgtcatccg cggccacaac cagcacgacc
     7201 acgtccgtca ggttggcgcc gcgtgcacgc atggccgtga acgcctcgtg ccccggagta
     7261 tcgaggaacg tcacgcgcca atcgccgcgc tccacccggt aggcgccgat gtgctgcgtg
     7321 atgccgccgg cctcgccagc ggccacgtcc gccttgcgaa tcgcatccag cagcgaggtc
     7381 ttgccgtggt ccacatggcc tagcattgcg acgaccggcg ggcgcggttt caagtgctcg
     7441 cggggccgtg cgtcgaattg ttccttcagg cgctcaaggg cggttttctg ctttgcgatt
     7501 gtcaccggca ccccgagctc catggttaac aattcaagcg tgtcggcatc tatattttcg
     7561 ttaatccgcc acagccggtt cgtgtgctcc agcagctttt gggaaacggt tgagaacgga
     7621 attccaacgg cggagcagaa ctcctttaac gctatcggcg cggtcagctc aataggcgct
     7681 ttacgggccg cagacacccc ggccgccgca gccgcggccc ggcggcgctc ggctgctcgt
     7741 cgctcgcgca accccatccc ggtcgcactt gccaaacgtt ctttgcgctc caggagatct
     7801 tgctcgtgcc actctttcag gcgttcgccc acatcgaccg tgcggccgtg acggcgagga
     7861 ctgcgcgacc ggattcttgt cccgtcaccc tcatccgcct gcccatgccc tcgagcgcga
     7921 ctcttgcggc cggctgcggt gccgggctgg gaaacgacgt cgatcgacgg tccacgagcg
     7981 gccggcgcct gacgcgggcg cggtgggcga acgggctctg gcgcctcgat tcggacgacg
     8041 cgcgggccct tcagttcggc cgggcgcggc actacctgtg ggccggctgg ctttacaggc
     8101 ggcggcggag gaggaggcgg gagtatcgtt ggcgcggggc ggggcggcgg ctcgggcgcc
//
LOCUS       DAOSYS010000164         8155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7496_length_8155_cov_4.833827, whole genome shotgun sequence.
ACCESSION   DAOSYS010000164 DAOSYS010000000
VERSION     DAOSYS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_8155_cov_4.833827"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="PLU99_10645"
     CDS             <1..136
                     /locus_tag="PLU99_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17554.1"
                     /translation="CYGGDITRKRKLLEKQKEGKKRMKTIGHVDIPQKAFMTVLEPED
                     "
     gene            378..1820
                     /locus_tag="PLU99_10650"
     CDS             378..1820
                     /locus_tag="PLU99_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17555.1"
                     /translation="MAGRPATIRGGGISGAQIGLIVFAFVSVAALGLFIFQLTRVKEL
                     QQRAQTAEARIKHLGQPAAPLGSYYDNEATARGSGATVFKLMTEDLERVSQLIAGESK
                     LIAKALEEQSNELLRQVAARHPISASDTLLTAIRKLDQHATELAETKKSQALELAEAQ
                     QNIESLTQQLKAARDEFEDQIADTKSDYERAVADFGTKLAAKDAQVRDLRELLESREK
                     DLQDLRRAAETDKREWELERTRRDKQIAGFQRKIEELAGTFDPEEILRKADGRVLRAI
                     PGSALVYINLGANNNIKVGMSFEVFSHTATPNRTLRGKGSIDVVNVMDETAECRITRN
                     DPAQPITEGDTIVNIAYEQGRKPKFVVCGNFDLNYDGQIDPDGAEEIKSMIRRWGGQV
                     MPELDETVDFVVIGTASKVPPLPPYASDIQRDQAERRSLENVEKFEPLKKQALAMHIP
                     VITQNQFFFLTGYVSQPDRSLYSDRSLGLD"
     gene            2019..3242
                     /locus_tag="PLU99_10655"
     CDS             2019..3242
                     /locus_tag="PLU99_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17556.1"
                     /translation="MFYLRLALTALRSLDSHFLRSLLATLGVLIGVGSVVACMSILEG
                     FSNDIAKRFKSLGSNLLYIHPTAASVEGRPVGLAQTLTLEDVRLLKRELADQIDTVAP
                     ECTGSATVKYFQNSYPAAVVIATSDEYFTMHAFEAQHGQVLNKAHSEDEGAAVVCLGA
                     KIADKLFGGMDPVGQTVKVGEKAYRVLGVMEKKGSLGFLDADEAVYLPIEAGLKRFFN
                     RKWLLRITVGIKDATRLEEVQKQVQRVLRASHHIRAGEDDDFRVFNQEESLQSFNQIM
                     LIWKIVFYNIAGISLLVGGIGIMNIMLVSVTERTREIGVRMAVGARRGDILLQFLLEA
                     LIISFLGGAFGLLLGAMFADLLNKVVVQFFRTQITPMVMITAVATSTVVGVLSGIYPA
                     LKASRLNPVDALRYE"
     gene            complement(3322..4950)
                     /locus_tag="PLU99_10660"
     CDS             complement(3322..4950)
                     /locus_tag="PLU99_10660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO17557.1"
                     /translation="MTPAFPSVSIVIVGPQGCSNAADLDGAIQFARAQDVPIAELIAV
                     GRPDDVAASAAATNTAPRLRILSEAFRNRAAACNAALREVSGEFVLFVFNDNGLVTLR
                     QSAARTMLLAATRHSANTAAPERFSAGLVYADYERVEPDGRRREIHLLDWHPGRLRDT
                     FDFGRAILFCTAALRDVGGFDESYDAADFYDLRLRLLEKYRPVHVANRFAGSLYTVAA
                     PAKAHNVFDYLLAGKPAQLEMERACTAHLKRTGAYLAPGAHLRRVTYSPQEESRFAEC
                     LASVVIPVYRRPGFMGRAIESVQAQTERRVEAIVVVNGGPDDPTAAEVRRYMPGGDRY
                     RPAAPPVSLIVLDVNNIGLCLNTGIAAARGKYYVQLDSDDRLKPDAVEKLLSVFESDP
                     AIGMVIGSYEVWTLDEQTGGLTRNAEIPVVTHDEWTADNGRNNLLRINGAGAPRAAHI
                     KLIRELGGFGLNDAPHSRNYGEDYDLVLRLSEHYAIGRVWEPIYEVIRHSGGTDHAID
                     QATIDRNDEAKDHMRLEALKRRRRLNANDQRSDK"
     gene            complement(5219..6580)
                     /locus_tag="PLU99_10665"
     CDS             complement(5219..6580)
                     /locus_tag="PLU99_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17558.1"
                     /translation="MRNLLAVLLSLAVPCFLMCGCVGAPIWGDNFWDIFAPILDGQGP
                     GNPSDGDDDNVDPSDGDNVTPGGDGQGKLTPVKTSIPLRFDAGLECGDDLIAFGTDVL
                     VGVSYVIPSQNPTAGTPVPDSDQYDCCNFDVAGRWIFMAGSNGGSLPYQVSAFNVDTL
                     EVTTFDAADIRLVKMVVKPRDAGRLQASGDYCVVVCDQDKVDDGKIIKVIDVSSGTPE
                     LITFEQNPVDYASVVTQVAVNGTTKTVAVAAEEALWIYDITQPTAAPRKIIPPNGVGK
                     YQMKMSGNYLIASSYDGGYDTAILVDLAANKVVTMEQAETIADLAIGGDKFAFFSHHD
                     SDDSVGLHFRTAVGTLPGPTFNKPPTDNYIDGSYTSNGVVGYAGTMCMTPGGEYLFLA
                     NFYLQYSQGNASFTVPKDPANADTYGCPAWDIDCSDNTVGFHSAASRSSREEGVLGYI
                     ILE"
     gene            complement(6905..8086)
                     /locus_tag="PLU99_10670"
     CDS             complement(6905..8086)
                     /locus_tag="PLU99_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO17559.1"
                     /translation="MTETRQPFDAAAPDYFQLRDLLTDDERQIQHVVARWVDERVLPI
                     IPTCFEQGRFPRELIAEMAGLGLFGPTLHDYGCAGLNNIAYGLIMQELERGDSALRSF
                     ASVQSGLVMYPIDRYGSDEQKQRWLPAMASGQAVGCFGLTEPDGGSDPGTMKTRARRE
                     GGDWVINGAKMWITNGTLADVAVVWAMTDHGVRGFLVERGTPGYTTRDIEHKFSLRAS
                     ISSELFFDNVRVPERSRLPGAEGLAAPLSCLTQARYGIAWGAIGAAQACYREALEFAR
                     QRVLFGTPLTHKQIIQRRLANMVRRITAAQLLAWRLGRLKDQGTMHHSQVSLAKWNNV
                     RMALDVAREARDILGAGGITTEFATIRHMLNLESVITYEGTETIHELIVGREICGVGA
                     F"
BASE COUNT         1434 a         2429 c         2772 g         1520 t
ORIGIN      
        1 gtgctacggc ggtgacatca cccgcaagcg caaactgctg gagaagcaga aggagggcaa
       61 gaagcgcatg aaaaccatcg ggcacgtgga tatcccgcaa aaagcgttca tgacggttct
      121 ggaaccggag gattgagcgc gcctgggcgt cgctgttggg ttatcggagg atgggccggg
      181 tgaggcttta atcggcgtgg cgtgcggagt tttgccgtcg ccggcgcttc gggctcggat
      241 cgtgagggcg gctgctttga caagtcagct tggctcttcc atactgaggt attgagccgc
      301 gagcgcgccg gtagtgccct tggttgggcg gcgcgccaga ccgcccgcgg cgggtggtca
      361 gtttagagga gttcaaaatg gcgggtcgac cggccaccat ccgaggcggc ggaattagcg
      421 gggcgcagat tggtttgatt gtcttcgctt tcgtctccgt cgccgcgctt ggcctgttca
      481 tcttccagtt gacgcgcgta aaggagcttc agcagcgggc ccagacggcc gaggcgcgga
      541 tcaagcacct gggccagccg gccgccccgc tggggagcta ctacgacaat gaagccacgg
      601 cgcgtggctc aggcgcaacg gtgttcaagc tgatgacgga ggacctggaa cgggtctcgc
      661 agcttatcgc cggcgagtcc aagttgatcg ccaaggcctt ggaggagcag tcgaacgagc
      721 ttttgcggca agtcgcggct cgccatccga tcagcgccag cgacaccttg ctgacggcca
      781 ttcgcaagct ggaccagcac gccaccgagt tggctgaaac caagaagagc caggcgctgg
      841 aattggccga ggcgcaacag aacattgagt cgctgacgca gcagcttaag gcggcgcgcg
      901 acgagtttga ggaccagatc gctgacacta agagcgacta cgagcgcgcg gtggccgact
      961 ttggcaccaa gctggccgcc aaagacgccc aggtgcgcga cctgcgggag ctgctcgaat
     1021 ctcgcgagaa ggacctgcag gacctgcgac gcgcggcgga aaccgataag cgtgaatggg
     1081 aactcgagcg tacgcggcgc gacaagcaga tcgcgggctt ccaaaggaag atcgaggagc
     1141 tggcgggaac gttcgacccc gaggagattc ttaggaaagc cgacgggcgg gtgctgcggg
     1201 ccattcccgg cagcgccctg gtctacatca acctgggcgc caacaacaac atcaaagtcg
     1261 gcatgagctt cgaggttttc tcgcacactg ccacgcctaa ccgcaccctg cgcggcaagg
     1321 gctcgatcga tgtggtcaac gtcatggacg agacggctga gtgccggatc acacgcaacg
     1381 accccgccca gcccatcact gaaggggaca ccatcgtcaa catcgcttac gagcagggcc
     1441 gcaagccgaa attcgtggtc tgcggcaatt tcgatttgaa ctatgatggg cagatcgatc
     1501 cggacggcgc cgaagagatc aagagcatga tccgccgctg ggggggccag gtcatgcccg
     1561 agctggatga aaccgttgat ttcgtggtca tcggcacggc ctccaaggtc ccgccgctgc
     1621 cgccctacgc atcggatatc caacgcgacc aggccgaacg ccgctcgctc gaaaacgtag
     1681 agaagttcga gcctctgaaa aagcaggcct tggccatgca cattccggtc atcacccaga
     1741 accagttttt cttcctgacc gggtatgtga gccagcccga ccgctcgctg tacagtgacc
     1801 gcagcctggg gctcgattga cggcctgccg cggttcttcc aatgttccgc ggctccttcg
     1861 aacacgccgc ccgtgcgggc gaggcggtgt cgttttctgt cgggttgtcg cgcgctgctc
     1921 gcccgcgggg gcttgctcgc gttatcattg gccaccgcta aaatgcggcg cgtctgacga
     1981 actgccgacc gctttgaagc cggaggcgcg taagctggat gttttatcta cggctagccc
     2041 tgaccgcgct gcgcagcctg gactcacact tcctgcgctc gctgctggcg accttgggcg
     2101 tgctgatcgg cgtcggctcg gtggtggcct gtatgtcgat cctggagggg ttctcgaacg
     2161 atatcgccaa gcgcttcaag tcgctgggct ccaacctgct gtacattcac ccgaccgcgg
     2221 cctcggtcga gggccggccg gtgggcctgg cccagacgct gacgctggag gatgttcgcc
     2281 tgctgaagcg cgaactggcg gaccagattg acacggtcgc gcccgagtgc accggttcgg
     2341 ccacggtcaa gtactttcag aacagctatc cggcggcggt cgtgatcgcg acctcggacg
     2401 agtacttcac gatgcacgcc ttcgaggccc agcacggcca agtgctcaat aaggcccatt
     2461 cggaggacga gggggcggcg gtcgtgtgtc tgggggcgaa gattgcggac aagcttttcg
     2521 gcgggatgga tccggtcggt cagaccgtca aggtgggcga gaaggcttac cgcgtgctgg
     2581 gcgtgatgga gaagaagggc tcactcggct tcctggatgc ggatgaggcg gtctacctgc
     2641 cgatcgaagc ggggctgaag cgcttcttca accgcaagtg gctgctgcgc atcactgtcg
     2701 gcatcaagga cgcgacccgc ctcgaggaag tgcagaagca ggtgcagcgc gtgctgcgcg
     2761 cctcgcacca cattcgcgcc ggcgaggacg atgacttccg cgtgttcaac caggaggagt
     2821 cgctgcagag cttcaaccaa atcatgctga tttggaagat cgtcttctat aacattgccg
     2881 gcatcagcct cctggtgggg ggcatcggca ttatgaacat catgctggtt tcggtcaccg
     2941 aacgcacccg cgagatcggc gtccgcatgg cggtcggtgc gcggcgcggg gatatcctgc
     3001 tgcaattcct gcttgaggcg ctgatcatca gctttctcgg cggggcgttc ggcctgctgc
     3061 taggcgccat gttcgccgac ttgctcaaca aggtggtggt ccagttcttc cgcacccaga
     3121 tcacgcccat ggtgatgatc acggcggtgg cgacgtcgac cgtggtaggg gtgctgagcg
     3181 ggatttatcc ggcgctgaag gccagccggc tgaatcccgt ggatgcgctt agatacgaat
     3241 aagtgccaaa agacgaattt caaatgaaca aatagaacac ccggcgcgcc agcgctcttg
     3301 ccgttggccg cctgtcatag gtcatttgtc acttcgctgg tcattcgcgt tcagcctgcg
     3361 gcgccgcttt agcgcctcca gacgcatgtg gtccttggcc tcgtcgttgc ggtcgatcgt
     3421 cgcctgatcg atggcgtgat ctgtgccgcc ggaatgacgg atcacttcat agatcggttc
     3481 ccaaacgcgg ccgatggcgt agtgctcgct caggcgcagc accaggtcgt agtcctctcc
     3541 gtaattgcga ctgtgcggcg cgtcgttcag gccgaacccg ccgagttccc ggatgagctt
     3601 gatgtgcgcg gcacgcgggg cgccggcgcc gttgatgcgc aggaggttgt tgcggccgtt
     3661 gtcggcggtc cattcatcgt gagtgacgac cggaatttcg gcattgcgcg tgagcccgcc
     3721 ggtctgctcg tccagggtcc agacttcgta cgagccgatc accatgccga tggccggatc
     3781 ggattcgaag accgaaagca atttttcgac cgcgtcgggt ttcagccggt cgtcggaatc
     3841 gagctggaca tagtacttgc cgcgtgcggc tgcgatgccg gtgttgaggc acaggccgat
     3901 gttgttgacg tccagcacaa tcaggctcac gggcggcgcg gcgggccggt aacggtcgcc
     3961 gccgggcatg tagcgccgga cttcggccgc ggtcgggtcg tcggggccgc cgttgacgac
     4021 gacgatggct tcgacgcgac gttcggtctg ggcctgcacg ctttcaatgg cgcggcccat
     4081 gaagcccggg cgccggtaga cgggaatgac tacgctggcc aggcattcgg cgaagcggct
     4141 ttcctcttgc ggcgaatacg tgacccggcg gaggtgtgct ccgggggcga ggtaagcgcc
     4201 ggtgcgcttc aggtgcgccg tgcaggcgcg ctccatttcg agctgagcgg gcttaccggc
     4261 gagcaggtag tcgaaaacgt tgtgagcttt cgctggggcg gccacggtgt acagcgaacc
     4321 ggcgaagcgg ttggccacat gcacggggcg gtacttttcc agcagacgca gccgcaggtc
     4381 atagaaatcc gcggcgtcgt aggactcgtc gaatccgccg acgtcacgca gggcggccgt
     4441 gcaaaagagg atggcgcggc caaaatcgaa cgtgtcgcgc agccggcccg ggtgccaatc
     4501 gagcaagtga atctcacgcc ggcggccgtc gggctcgacc cgttcataat ccgcgtagac
     4561 caggccggcc gagaagcgtt ccggcgcggc ggtattagcg gagtggcgcg tggccgcgag
     4621 caacatggtt cgcgcggcgg actgcctcag ggtcaccagt ccgttgtcgt tgaacacgaa
     4681 cagcacgaac tcgcctgata cttcgcgcag cgcggcgttg cacgcggcag cacggttgcg
     4741 gaaggcttcc gagaggatgc gcaggcgcgg ggcggtgttc gtggcagcgg cggaggctgc
     4801 aacgtcgtcc gggcggccca cagcgatcag ctcagcgatg ggtacgtcct gggcgcgggc
     4861 gaattgaata gcgccgtcca aatcagcggc gttcgagcag ccctgcgggc cgacgattac
     4921 gattgaaacg gacgggaagg ctggcgtcat gggtgacaat cttaagcgcc tcgactacgg
     4981 cagcgataca tcccagcgat ttactggccg tggcgagatt ctaccagttg cgggcagtcc
     5041 gcggacgact cagaacggcc cggctgcgct tgcgccgccc gtcgaaaaca ccgccggccg
     5101 cgcgggcttg cccggcggcc ggcgattggg gaaaggtgcg tcttgtatgc gcggcctttt
     5161 cttccttgtg gatgcggcct ctcagccgct ccgcaagcga actagccgcg ccgagtttct
     5221 attcgaggat gatgtatccc agcacgccct cctcgcgcga gcttctcgag gcagcgctgt
     5281 ggaagccgac agtgttatcg ctgcagtcga tgtcccaggc cgggcagcca taggtgtcgg
     5341 cgttggccgg atccttggga acggtaaacg acgcgttgcc ctggctgtac tgcaggtaaa
     5401 agttggccag gaacaggtat tcgccgccgg gcgtcatgca catggtcccg gcgtatccga
     5461 ctacgccgtt actggtgtag ctgccgtcga tgtagttgtc tgtcggcggc ttgttgaagg
     5521 ttgggccggg cagcgtgccg accgccgtgc ggaagtgcaa gccgaccgag tcatccgaat
     5581 cgtggtggga gaagaacgcg aacttgtcgc cgccgatcgc caggtcggcg atggtttccg
     5641 cctgctccat ggtgacgacc ttgttggcgg cgaggtcaac gagaatggcg gtgtcatagc
     5701 cgccgtcgta cgatgaagcg atgaggtaat tgcccgacat cttcatctgg tacttgccga
     5761 cgccgttggg gggaatgatc ttgcgcgggg cggcggttgg ctgcgtgatg tcgtaaatcc
     5821 agagcgcctc ctcggccgct accgcgacgg tcttggtggt tccatttacg gccacttgcg
     5881 tgaccacgct ggcgtagtca accgggttct gctcaaaggt tatcagctcg ggtgtgccgc
     5941 tgctgacgtc gatgaccttg ataatcttgc cgtcatcgac cttgtcctgg tcgcagacca
     6001 cgacgcagta gtcgccgctg gcttgcagcc ggccggcatc gcggggcttg accaccattt
     6061 tgaccagacg gatatccgcc gcatcgaagg tggtcacctc gagggtgtcg acgttgaagg
     6121 cgctgacttg ataggggagg ctgccgccat tgctgccggc catgaagatc cagcgtccag
     6181 cgacgtcgaa gttacagcag tcatactggt cgctgtcggg gacgggcgtg ccggccgtcg
     6241 gattctggga cgggatgacg taggaaacgc cgaccaggac gtcggtgccg aaggcgatca
     6301 ggtcatcgcc gcattcgagg cccgcatcga accgcagggg gatagaggtc ttgacggggg
     6361 tgagcttgcc ctggccgtca ccgccgggcg tgacgttgtc accgtcgctc ggatcgacgt
     6421 tgtcgtcatc accgtcgctg ggattgcccg ggccctggcc gtcaagaatg ggggcaaaga
     6481 tgtcccagaa attgtcgccc cagatcggcg cgccgacgca accgcacatc agaaaacacg
     6541 ggaccgccaa actcagaagc actgccagca aattcctcat catgatctcc aagaaaagag
     6601 ttacgatcgg cccttgcgca cgcccttcga tgcgccttaa gagcggtgcg ctgggcgggc
     6661 tttcgttgtg cgagctgtgc gtcggggcag actgcgctgg cccgagaaac gaggcacggc
     6721 agcccgcaat acagggtttc ttttcggtcg ttttggggcc cgggtttgag taaaagtgcg
     6781 aggaaaggca accggcggcg agacggtgag gcgaccaggc gacgaggtct gataaaatgg
     6841 tccgcttggt cgccgccgtg cggttcagta aaagcgggtc aaccgagagg cgcggcctcc
     6901 cggttcagaa cgcgccgact ccgcagattt cgcgtccgac gatcagctcg tggatcgtct
     6961 cagtgccctc gtaggttatg acgctctcga ggttcagcat gtggcggatc gtggcgaatt
     7021 cagtcgtgat gccgccggca cccaggatgt cgcgggcctc gcgggcgacg tccaacgcca
     7081 tgcgcacgtt gttccacttg gccagcgaaa cctggctgtg gtgcatcgtg ccctggtcct
     7141 tcagccgccc gagccgccag gccagcagtt gcgcggcagt gatgcggcgc accatgttcg
     7201 ccaggcgccg ctgaatgatc tgcttgtgcg tcagtggcgt tccgaagagc acgcgctggc
     7261 gcgcgaattc cagcgcttcg cggtaacagg cctgcgccgc cccgatcgcg ccccaggcaa
     7321 tgccatagcg ggcctgcgtc aggcagctca gcggcgcggc caggccctcg gcgcccggta
     7381 gccggctgcg ctccggcacg cggacgttgt cgaagaacag ttctgagctg attgacgccc
     7441 ggagcgagaa tttgtgctct atgtcgcgcg tggtgtagcc cggcgtgccg cgttcgacca
     7501 ggaagccgcg cacgccgtgg tccgtcatgg cccagacgac cgcgacgtcc gcaagcgtgc
     7561 cgttggtgat ccacatcttg gccccgttta tgacccagtc gccgccctcg cgccgggcgc
     7621 gggtcttcat cgtgccgggg tcggagccgc cgtcgggctc agtcaggccg aagcagccga
     7681 cggcctgccc gcttgccatg gccggcagcc agcgctgctt ctgctcgtcg gagccgtagc
     7741 ggtcgatggg gtacatgacc aggccgctct ggacggaggc gaagctgcgc agggcgctgt
     7801 cgccgcgttc cagctcctgc atgatgaggc cgtaggcgat gttgttcagg ccggcgcagc
     7861 cgtagtcgtg gagcgtcggt ccgaagagtc ccaggccggc catttcggcg atcaactcgc
     7921 gtgggaagcg gccctgctcg aagcaggtgg gaatgatggg caggacgcgc tcgtcgaccc
     7981 acctggcgac tacgtgctga atctgccgct cgtcgtcggt cagtaggtca cggagctgga
     8041 agtagtcagg ggcggccgcg tcgaacggct ggcgagtttc ggtcactgtg ggctcctgtc
     8101 ggttttgcac tatgagcgcc tgtcggtctt gggtgcgctt ttcctacgat tgtaa
//
LOCUS       DAOSYS010000165         8073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7590_length_8073_cov_4.812173, whole genome shotgun sequence.
ACCESSION   DAOSYS010000165 DAOSYS010000000
VERSION     DAOSYS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7590_length_8073_cov_4.812173"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLU99_10675"
     CDS             <1..838
                     /locus_tag="PLU99_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOO17560.1"
                     /translation="KSNNLSCVLRVEAGGRAIAVLGNGLTGIYPPENQALAEKILENG
                     AWFSELPMKASVRRENFPVRNRIIAGMSLGTLVVEAATRSGALITARLASDYNREVFA
                     VPGRLQEPMAEGVNALIRDGIAKLVTGLDDILEGLGEVGYQLRLKLYGPARRDTETTP
                     REGEAVARAGEAVRPEGETAPREGGATLWEREAAAETVASATEPGASGGPQTPPPGPT
                     LSPAEKRVFEVIPYDAILQDMVIRAAELPPGEVLAALTTLELKGLIKRLPGNLVMRRA
                     RA"
     gene            1070..1540
                     /locus_tag="PLU99_10680"
     CDS             1070..1540
                     /locus_tag="PLU99_10680"
                     /inference="COORDINATES: protein motif:HMM:NF040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17561.1"
                     /translation="MTGSSTIIVLGGPSAGKTVYLSVLYHHLWHGSDGMIMRAGSGAM
                     HTELLGSVEALKRGTMPPATQALRPYEFELERNGQKYHLRCLDYPGELFRRVFFDLAI
                     DSDEARELMRFCEDAAGVIVLADPASTSAPRTGKATLSFCSTAPRGQSRTTVFC"
     gene            1537..2052
                     /locus_tag="PLU99_10685"
     CDS             1537..2052
                     /locus_tag="PLU99_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOO17562.1"
                     /translation="MTCKHVLPPPEAQAHLPLRTWVYLNNERFDAELVWRDTVADLAL
                     LKVDAYIPYRFRIAPKGTVQRLNVSVRVIGFQEMPTEIDSPVRDTPLASANGTISRLY
                     QDKVGTWWIEHTAPVKAGGSGGPVALGDSVVGLSCTGDRGYYQAIDTTVYGEKIAQVI
                     KDFRKEAQHHE"
     gene            2045..2203
                     /locus_tag="PLU99_10690"
     CDS             2045..2203
                     /locus_tag="PLU99_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17563.1"
                     /translation="MSEARWTYSDRQVIIAVSIALLICLAFLAGFAVGDMEWVARIRS
                     SLLQMLQR"
     gene            2416..3396
                     /locus_tag="PLU99_10695"
     CDS             2416..3396
                     /locus_tag="PLU99_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOO17564.1"
                     /translation="MRKLLVTTAIFAAVALAAARAETVVLKGGQRVTGEVLREYPQHI
                     VVDLGFDLLSIPRDEIQGINPDEPTDTQPAECVQATPDLYSTARLPVDGVKQLTERFG
                     EGVVLVSVPGALGSGFFISEHGHVITNFHVIEGETRIAVTVFRKVGDEFKRQKFEDVD
                     IIAINPVVDLALLRVDLPKDYKPVLTYLATGDDVRDGDAVFAIGNPLGLERSVSEGII
                     SKHNRAEEGMVYIQTTAQINPGNSGGPLFNNRGEVIGVNTWKIMFSEGIGFAIPIRYV
                     LDFLRNRDAFAYNSETSTAGYRYMQPAPRGKLEAPVFLKQDDSIQVKGDK"
     gene            3393..5240
                     /locus_tag="PLU99_10700"
     CDS             3393..5240
                     /locus_tag="PLU99_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17565.1"
                     /translation="MKRALGLTFAGLFLLLTPAALAQTTREAAPDPAEWVPADALFYL
                     GIPDTAQLWKDFQNTVAYKMSQDKELEEMPSMPGVWGKLAKTLKERLAQLVEVDVESL
                     KNPLAGPLAFYIAAAPGGGLDTVEPVVVAGVGDQQLMKQYFDSAIARLKTAASSYETV
                     TAAGHTIHVFKGAKPAAEEKAQEDEREFELEPEVDPEGFNEALADKLDDAWGLLSPDK
                     LPEKLALCFAGDRLIASATPDRIKSVLARERGGETLAATEDHKLLLSSLKPVGQIRLL
                     GNLPRLFEMLQRDAEGEEAASLRQTLDIIGAGSLRAAVGHCRLGSKSYDSKIDVLFLM
                     SGERTGLARILSMENKPLDPPSWTTSDTAIFASFNFDPPKLIDEVLRIVAQTDPKAAE
                     EARAAMEQVPMPPDGQPINLRKDFIDHLTGPLVGAVDMARPYGPGSLRGMVSLGHRNR
                     DAITRVLSPVVGLTQEPRDVRGSLVFTVPQTGGALAIGNDRLLLGSQAVVESALSAGT
                     SGGLAEDASFRRAQRNVPKEAWCLVYIDQRKLMQGVIELARKKEQLAGDLAATITLMM
                     LQNTGMDFSDEKLSQKMLQYSAPSIFTISTTTQGIHVSMVGLNPGQEED"
     gene            complement(5392..5730)
                     /locus_tag="PLU99_10705"
     CDS             complement(5392..5730)
                     /locus_tag="PLU99_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17566.1"
                     /translation="MSAEHTELAAGNVELDELDRAVREGRLTRVVLESPEQLLAGLWN
                     GEIDAQTWLTAGVEVEFATKVDGSALPHLLLESWSVNARRHRRRRIVAGAVLSIIAIA
                     AAFILLWAAA"
     gene            complement(5727..6500)
                     /locus_tag="PLU99_10710"
     CDS             complement(5727..6500)
                     /locus_tag="PLU99_10710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOO17567.1"
                     /translation="MLVPVCMWGGQSIMLRRAGLPIRLRIGADDLPPGLRRWNRVITQ
                     AAFAAVLIGYPLLCGQSPVAYYGGYLPLGRRPLELLYGAAAAVLYLCLLYAAWVASDN
                     VHFRARHAGPRLLRRIAAAPLTAVFAAGVEELLFRAVLLVGLLETFDEVSAMAIGAML
                     FAGAHYIRRVKRYWTFAGHVVLGVLLCAAFVWTKALWLPLGLHAGGVLVLAGVRPLVR
                     YTGPAWLVGASIYPYAGVVGITALLLLTVNIWLAFGGGS"
     gene            complement(6555..7556)
                     /locus_tag="PLU99_10715"
     CDS             complement(6555..7556)
                     /locus_tag="PLU99_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17568.1"
                     /translation="MAVLRQARKWLTGRVDDLAVSCAPWLSARSLDRVGPLIAWCGPR
                     LPVLGRLAAQNMSSAGLYSPQIHRDYFANVGAHLAGALQALRCAGRSQQGPQSDLKNL
                     AENCIQLDDSVQALAEAAGRGRGAILVGPHISNYLLNLTRLNQAVPLTIYLRHSKDSS
                     RQQAKQRWYRASGVSWISEPAGQNGPLGRLGQMSAVLRAGRVLFITPDLAKKRGEGVA
                     VRLLGREVYLPAGAAALAARTGAPLFALHARKEHRVQKLFVRGPFNLDSSLLRSRDPS
                     QAGRRTPALIAAGMQWFADEFERFLREQPALWYLWADKRWTRVFRGDPRYVGPGASL"
     gene            complement(7575..>8073)
                     /locus_tag="PLU99_10720"
     CDS             complement(7575..>8073)
                     /locus_tag="PLU99_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17569.1"
                     /translation="TSGAFSFTSLPVDTEGMLTYSAVGLPCTVLQAAGLLCSDTQSLD
                     QGTQTADEFGGTITSASRVVTLATNIDVLAPLDPAYPDMGTLHVWGVVRGSVYVPPPA
                     AGDTNCDGAVNVFDIDPFVMALTDPAGYASAFPTCEISAADTDGNGEVNVFDIDPFVL
                     LLTGG"
BASE COUNT         1574 a         2676 c         2470 g         1353 t
ORIGIN      
        1 gaagagcaac aatctgtcct gcgtcctgcg ggtcgaggcc ggcgggcggg ccattgccgt
       61 gctcggaaac gggctgaccg gtatttaccc gcctgagaat caggctctgg ccgaaaagat
      121 tctggagaac ggcgcgtggt tcagcgagct gccgatgaag gccagcgtgc gccgcgagaa
      181 tttcccggtc cgtaatcgca tcatcgccgg catgtcgctg ggcacgctgg tcgtcgaggc
      241 cgccacccgc agcggggcgc tgatcaccgc ccggctggcc agcgactaca accgcgaggt
      301 cttcgccgtc ccgggacgcc tgcaggagcc gatggccgaa ggcgtcaacg ccctcatccg
      361 cgacggcatc gccaaactgg tcaccggtct ggacgacatc ctcgaaggcc tgggcgaagt
      421 cggctatcaa ctcagactaa agctctacgg ccccgcgcgt cgggacaccg agacaacacc
      481 tcgcgagggc gaggcagtgg cccgcgcggg cgaggcagtg cgcccggaag gcgaaacagc
      541 gccgcgggag ggcggggcga cactctggga gcgcgaggct gctgccgaaa cggtggcatc
      601 ggccactgaa cctggcgcga gcggcggccc ccaaacgccg cctcccggtc caaccctctc
      661 accggccgaa aagcgcgtct tcgaggtgat cccatacgac gccattctcc aggacatggt
      721 catccgcgcg gccgaattgc ccccgggcga agtcctggcc gcactgacga cactagaact
      781 gaagggcctc atcaagcgtc tgccgggcaa tctagtaatg cgccgcgcgc gagcatagag
      841 ccgagccgcc gggtgtcgct tgggaatgcc gcccagcgca atcgcgcaag cggtagtatg
      901 tcgcttacac tggcgtgaaa atccgtccca cacccattcc tccttgacct ttgcgaaacg
      961 taagccgctt gctgtgaact tgattttcgt tatgctgttg tcgtagaggc tacagctcgt
     1021 ctctcgggag ctgcgccaag tcgccccagc ttgcggtgtt gggaaagaaa tgaccggttc
     1081 ctcgacgatc atcgtcctgg gcggaccgtc cgctggtaag acggtttatc tcagcgtgct
     1141 ctaccaccat ctgtggcatg ggtccgacgg catgatcatg cgcgccggca gcggcgcgat
     1201 gcacaccgaa ctgttgggct cagtggaggc tctcaaacgt ggaacaatgc cgccggctac
     1261 acaggcgctg cggccttatg aattcgagtt ggagcggaac ggccaaaagt accatctgcg
     1321 ctgcctggac tacccggggg aactgttccg ccgcgtattc tttgatttgg caatcgacag
     1381 tgacgaagcg cgcgagctga tgcgcttctg cgaggacgcc gcaggcgtaa tcgtgctggc
     1441 ggatcctgcc agtacgtcgg caccgcggac gggcaaggcg acattgtctt tctgcagtac
     1501 ggcaccgcgt gggcagtctc gaacgacggt tttttgttga cctgcaagca cgtcctgccg
     1561 ccgcctgaag cgcaagcaca cctcccgctg cgcacctggg tttatttgaa taacgagcgc
     1621 ttcgacgccg agcttgtctg gcgggacacc gtcgctgatt tggcgctcct gaaagtcgac
     1681 gcttacatcc cctaccgctt tagaatcgcg ccgaagggaa cggtccagcg actcaacgtg
     1741 tcggtgcggg tcatcggttt tcaggagatg ccgacagaaa tcgattcccc cgtgcgcgac
     1801 acgccgctgg ccagcgccaa cggcacgatc agtcgcctct accaggacaa ggtcggcaca
     1861 tggtggatcg agcacaccgc gcctgtgaaa gccggcggca gcggcggccc ggtggcgctg
     1921 ggcgactcgg tcgtcggact aagctgcacc ggcgaccgcg gctactacca ggcgattgat
     1981 acaactgtct acggcgagaa aattgctcag gtgatcaagg atttccgtaa agaggcgcag
     2041 caccatgagt gaggcccggt ggacctactc ggacaggcag gtgatcatcg cagtcagcat
     2101 cgcgctgctg atttgcctcg catttctggc cggctttgcc gtcggcgaca tggaatgggt
     2161 cgcgcggatt cgcagctcgt tgctgcaaat gctgcagcgc tgaaagcgcg gcgccgcaaa
     2221 tgcgttaccg cccaagcgct ggacctccaa acgagctgcg gtccccgttc atctcttgat
     2281 ccctcgatcc cttgatccct cgatccctgt ttttattgga cagcgccagc cttgttttcc
     2341 ccgccgcgct ttcttacgat tccacccaga agccgggaca agaacaggcc ccttttcgct
     2401 tttctgaggg tccgtatgcg caagttattg gttactaccg caattttcgc cgccgtcgct
     2461 ttggccgccg ctcgggccga gaccgtcgtc ctcaagggcg gccagcgcgt cactggcgaa
     2521 gtgctccgcg aatacccgca acatatcgtt gtcgatcttg ggttcgacct gctttccatc
     2581 ccgcgcgatg aaatccaggg cattaacccc gatgagccga ccgatactca gcccgccgaa
     2641 tgcgtccagg ccacacccga cctttacagc acggcccgcc tgcccgtcga cggcgtcaag
     2701 cagctcaccg aacgcttcgg cgaaggcgtc gtcctggttt ccgttccggg cgccttgggc
     2761 agcggcttct tcatcagcga acacggccac gtcatcacca acttccacgt gatcgagggc
     2821 gaaacacgca tcgcggtcac ggtcttccgc aaagtcggag acgagttcaa acgccagaag
     2881 tttgaggacg tggacatcat cgccattaat cccgtcgtcg acctggcgct gctccgcgtc
     2941 gatctgccca aggactacaa gccggtgctg acatacctcg cgaccggcga cgacgtgcgc
     3001 gatggcgacg cggtcttcgc catcggcaac ccgctcggcc tggagcgcag tgtcagtgaa
     3061 ggcatcatca gcaagcacaa ccgcgccgag gagggcatgg tctacatcca gactactgcc
     3121 cagattaatc cgggcaattc cggcggcccg ctcttcaaca accgcggcga agtcatcggc
     3181 gtgaacacct ggaagattat gttctccgag ggcatcggct tcgccatccc catccgctac
     3241 gtgctcgact tcctgcgcaa ccgcgacgcg ttcgcctata acagcgaaac ctccaccgcc
     3301 ggctaccggt acatgcaacc cgctccacgc ggcaagctcg aagcccccgt gttcttgaag
     3361 caagacgact ccattcaagt gaaaggtgac aaatgaaacg cgcgctcggc ctgacgttcg
     3421 ccggcctctt cctgctcctc acgcccgccg ctctcgccca gacaacccgc gaggccgccc
     3481 ccgatccggc cgaatgggtc ccggcagatg cgttgttcta cctgggcatc cctgacacgg
     3541 cccagttgtg gaaggacttc cagaacaccg tcgcctacaa gatgagccag gataaagaac
     3601 tggaagagat gccctccatg ccgggcgtct ggggcaagct ggccaagacg ctcaaggaac
     3661 gcctggcgca actggtcgag gtggacgttg agagtttgaa aaacccgctg gccggcccgc
     3721 ttgccttcta tatcgccgcg gcgcctgggg ggggactcga cactgttgag ccggtggtcg
     3781 tggcgggcgt cggcgatcag caattgatga agcagtattt cgactccgcc attgcccgcc
     3841 taaaaacggc cgccagcagc tacgaaactg tcaccgccgc cggccacacc attcatgttt
     3901 ttaagggagc gaagccggca gcagaggaaa aggcccaaga ggatgagcgc gagttcgaac
     3961 ttgagccaga agtggaccca gagggtttca atgaggccct ggccgataaa ctggatgacg
     4021 cttgggggct gctctcgccc gacaagttgc cggagaaact ggccctgtgc ttcgccggcg
     4081 accggttgat tgcgtccgcc acgcccgacc ggattaagag tgtgctggcg cgcgagcggg
     4141 gcggcgagac cctggccgcc accgaagatc acaaactact gctgagcagc ttgaagcccg
     4201 tcggccaaat tcgccttttg ggtaacctgc cgcgtctgtt cgagatgttg caacgcgacg
     4261 ccgaaggcga ggaagctgcc tcattgcgcc aaaccctcga cataatcggc gccggcagcc
     4321 tgcgggccgc cgtcggtcat tgccggctgg gcagcaaatc gtatgactcc aagatcgacg
     4381 tcttgttctt gatgagcggc gagcgtaccg gcctggcccg catcctcagc atggagaata
     4441 agccgctcga cccgccctcg tggacaacct cggataccgc cattttcgcc agcttcaact
     4501 tcgacccgcc caagctcatc gatgaggtgc tgcggatcgt agcccaaact gatccgaagg
     4561 cggccgaaga ggcccgcgcc gcaatggagc aggttcccat gccgcccgac ggccagccca
     4621 taaacctgcg caaggatttc attgaccatc tgaccggacc gctggtgggg gcggtggaca
     4681 tggcccgtcc gtatggcccg ggttcgctgc gcggcatggt gtcgctgggc catcgcaacc
     4741 gcgacgcgat tacacgcgtg ttgtccccgg ttgtaggact tacgcaggag ccgcgcgacg
     4801 tgcgcggcag cctggttttc accgtgccgc aaaccggcgg agcgctggcg atcggcaacg
     4861 accgcctgct gctcggcagc caggcggttg tggagtccgc actatcagcc ggcacgtcgg
     4921 gcggcttggc ggaggacgcg tccttccgcc gtgcgcagcg taacgtgccg aaggaggcct
     4981 ggtgcctggt gtacattgat cagcgcaagt tgatgcaggg cgtgatagaa ctcgcgcgca
     5041 agaaagagca gttggcgggt gatctggccg cgacaataac gctgatgatg ctgcaaaaca
     5101 ccggcatgga cttctcggat gaaaagctca gccagaaaat gctccagtac tccgcgccct
     5161 cgattttcac gatcagcacg accacccaag gcatacacgt ctcgatggtc ggcctgaatc
     5221 cggggcagga agaagactag agcgcgaagc gggagagcaa agcctcagca ggagggcgaa
     5281 ctgtactggg ccgccgccgg catttcgggg cggcacgccg gaggcagggt gagtccctgc
     5341 acaaataagg gattttggcg gcatgccggg gcggcacgcc ccggctctgc tctaggcggc
     5401 ggcccagagt aggatgaatg cggccgctat ggcaatgatg ctcaagaccg cgccggccac
     5461 aatgcggcgg cgacggtgcc ggcgggcgtt gacgctccag ctctccagca gcaaatgcgg
     5521 cagcgcggac ccgtcaacct tggtggcgaa ctccacctcc acgccggccg tgagccacgt
     5581 ctgcgcgtcg atctcgccat tccacagtcc cgccagaagt tgttccggcg actcgagcac
     5641 aacgcgcgtc aaacgcccct cgcgtaccgc gcgatcaagt tcgtccagtt ccacgttgcc
     5701 cgccgccaat tcggtatgct ccgcgctcat gacccgcccc caaaggccag ccatatgttc
     5761 actgtcaaca acaacagcgc cgtaatgccc accacccccg cgtaggggta aatgctcgcc
     5821 ccgaccagcc aggccggccc tgtgtagcgc accagcggcc gcacccccgc cagtacaagc
     5881 acgccgcccg cgtgcaaccc aagcggcagc cacagcgctt tggtccagac gaatgcagcg
     5941 cacaacagta cgcccagcac aacgtgcccg gcgaatgtcc agtagcgctt gacgcggcga
     6001 atgtagtgcg cccccgcgaa tagcatcgcc ccgatggcca ttgcgctcac ctcgtcaaac
     6061 gtctccagca gcccgaccag cagcaccgcc cgaaaaagaa gttcctcgac gccggccgcg
     6121 aacacggccg tcagcggcgc ggccgcaatc cgccgcagca gccgtggacc ggcgtgccgc
     6181 gcgcgaaagt gaacgttgtc gctggccacc catgccgcgt acagcaggca cagatagaga
     6241 accgccgccg cggctccata tagcaattcg agcggcctgc gccccagcgg cagatagccg
     6301 ccgtagtagg cgaccggcga ttgcccgcac agcagcggat aacctatcaa cactgccgcg
     6361 aacgcggctt gagtaatgac acgattccag cgccgcaatc ccggcggcag gtcatccgcc
     6421 ccaatccgca ggcgaatcgg cagccccgcg cggcgcagca tgatggactg cccgccccac
     6481 atgcatacgg gaaccaacaa gagtaccgcc aaagccacca ttcacgaagg ataaccgccc
     6541 gcccggcaag ctggctacag cgacgcgccg ggccccacgt agcgcgggtc gccgcggaac
     6601 acgcgcgtcc agcgcttgtc cgcccacagg taccacagtg ccggctgctc gcgcaggaag
     6661 cgctcgaatt cgtctgcgaa ccactgcatg cccgccgcga tcagagccgg ggttcgtcgc
     6721 ccggcttgag agggatcacg ggagcgcagg agcgaggaat caaggttgaa cggaccacgc
     6781 acaaagagct tctggacgcg gtgttctttc cgcgcatgca acgcaaacag tggagccccc
     6841 gtccgcgccg ccagcgcggc ggcgcctgcg ggcaagtaca cctcgcgccc caacagtcgc
     6901 actgcgacgc cctcgccgcg cttctttgcc aggtcgggtg taatgaacag cacgcggccg
     6961 gctcgcaaga cggcgctcat ctgtcccagc cggcccagcg gcccgttctg accagccggt
     7021 tcggaaatcc aacttacgcc gctggcgcga taccagcgtt gctttgcctg ctgccggctc
     7081 gaatccttcg aatgccgcag gtagatcgtc agcgggacgg cctgattgag gcgcgtcagg
     7141 ttcagcagat agttcgagat gtgcgggccg accagaatcg ccccacgccc gcggccagcc
     7201 gcttctgcca gcgcctgaac ggagtcatcc aattggatgc agttttcggc cagatttttc
     7261 agatcgcttt gaggcccttg ctgcgagcga cctgcgcagc gcaaagcctg taatgcgccg
     7321 gccaggtggg ccccgacatt ggcgaagtag tcgcggtgaa tctgcggaga gtacaggccc
     7381 gcggaagaca tgttctgcgc cgccagccgg cccagcactg ggagccgcgg accacaccag
     7441 gcaatcagcg gtccaacgcg gtccaacgat cgcgctgaaa gccagggggc gcacgacacg
     7501 gccaagtcat cgacgcggcc agtcagccat ttgcgcgcct gtcgcaatac cgccattccc
     7561 gcgcgcggcg tggttcaacc gccggtcagc agtaggacga atggatcgat gtcaaacacg
     7621 ttcacttcgc cgttgccgtc ggtgtccgcg gcgctgattt cgcacgtagg aaaggccgag
     7681 gcgtatccgg cagggtcggt caacgccatt acgaatggat ctatgtcgaa tacgttgacc
     7741 gcgccgtcgc aatttgtatc gccggcggcc ggcggcggca cgtacaccga gccgcgcacc
     7801 acgccccata catgcagcgt gcccatgtcc gggtaagccg gatccagcgg cgccaagacg
     7861 tcgatgttgg tcgccagcgt caccacgcga ctcgcggacg tgatcgtgcc gccgaactcg
     7921 tcggccgttt gcgtcccctg atccaagctt tgcgtgtcgc tgcaaagcag gccggccgcc
     7981 tgcaaaaccg tgcagggcag gccgacggcg ctgtacgtga gcatgccctc ggtatccacc
     8041 ggcaggctcg taaagctgaa agctccgctg gtt
//
LOCUS       DAOSYS010000166         8052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7621_length_8052_cov_5.824684, whole genome shotgun sequence.
ACCESSION   DAOSYS010000166 DAOSYS010000000
VERSION     DAOSYS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7621_length_8052_cov_5.824684"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /gene="pyrG"
                     /locus_tag="PLU99_10725"
     CDS             <1..1514
                     /gene="pyrG"
                     /locus_tag="PLU99_10725"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HOO17570.1"
                     /translation="LDQNLARLNFVTSGQIYRRVLERERIGGYLGRDVQMIPHVTGEV
                     KDRLRELAIASRADVVFVEIGGTVGDVENAYYIEAMRQLAFEEGPRSVCFVALTYVME
                     PSILGEQKSKAAQLGIRRLLECGIQPHIIACRAQNPVTHKVREKIAMFSNVPPDRVFS
                     MHDCESVYQIPEMLRGAGYDRAVIEWLRLENKVDEQAERASREVWGRYLARLRSAEHQ
                     VTIGITGKYTAVRDAYASILKALEHAGVHWGCKVHVRWIDTSHLNDQTVSEAVKGLDG
                     IIVPGAFGTRGAEGKIACLKYARMSKLPCLGICYGFQMAVIEFARNVCGMEDANSTEI
                     DTECRYPVINLLPEQKKIEGLGGTMRLGGQDVHVRKGTLAWRMFGERTRMRFRHRYEV
                     DPRYIPQLEEHGLIFSGRSPRAEIMNILELPQEMHPYYVGTQAHPELISRPLRANPMF
                     AGLVHAALRRAYPDFTAPLTFAGLMERENGAEPAVGEAEVVAADLVAREQSKA"
     gene            complement(1663..3759)
                     /locus_tag="PLU99_10730"
     CDS             complement(1663..3759)
                     /locus_tag="PLU99_10730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOO17571.1"
                     /translation="MTTEQREHDSTHPFSVHDMLAMDRIGEPQVSPDARHIAFTLRVT
                     DLEANKGRTDLWLVGIDGTGLRRLTTHPAGCSNPRWSPDSRAIYFISARNGGSQVWRI
                     SIDGGEAEQVTHLPLDVGCFAISPDGRHLAVSIDVFVDAATLDETKARLEEISKRKST
                     GRIYERLFVRHWDAWKDGRRAHLFVLPIEPVVQSPTNSGGAPIDVMKGMDADVPSKPF
                     GGAEEFTFTPDGREIVFSARNVGREEAWSTQFDLYAAPIDGSAPPRLLTPGNQAVITR
                     PVFSPDGKSLAYLAMQRPKYESDRQRIVVAESRQVAEPGQVAEPGRVAPENAKAPPAR
                     VLTEQWDRSAAELAWSPDGATIYTCAANLAQHSLFAVDVATGAVRTVLFEGHITDIAA
                     AGSRIVFALHHLRQPAELYSINPDGTDLRQITHINAAKVAAARMGEPEQFTFKGWNDE
                     TVYCQIVRPIDFDPQKKYPVAFLIHGGPQGSFGNDFHYRWNPQAYAGAGYAAVMVDFH
                     GSVGYGQAFVDSINGHWGDRPLEDLQKGLEAALARYPWMDGERVAALGASYGGYMINW
                     LHGVWPGRFRCLVCHDGNLDERMAYYDTEELWFPEWEHGGPPWERPEEYTKHNPIDHV
                     QNWGTPTLVIHGGQDFRVVDTQGLSTFTALQRRGIPSKLLYFPDENHWVLKPHNSIQW
                     HETVIAWLDQWCRRSE"
     gene            complement(3879..4715)
                     /locus_tag="PLU99_10735"
     CDS             complement(3879..4715)
                     /locus_tag="PLU99_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17572.1"
                     /translation="MNLVNAVLTNLTELLWYPLRPLPGVLVLVIWAAIAGALAGLMFR
                     YTSNQKALARVGDQIKANLLAMRLFKDDLRVTFRSQAGLLKAAGLRFWYALPAFVAML
                     VPFVLLLVQLALRFEYRPLRPGEVAIVELKLAPEAWETGQNAALETPPGVIIQTPALR
                     DARENAIYWRISPQDGATSGRLRWQVAGQTVEKELAVCDDPQRPCTVSMQRPGVSFWD
                     RLLHPGEPAFPADSPVRAAIVHYPPRQTPVFGFDIPWWATFLVASILMAFVLKPVLKV
                     RF"
     gene            complement(4910..5095)
                     /locus_tag="PLU99_10740"
     CDS             complement(4910..5095)
                     /locus_tag="PLU99_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17573.1"
                     /translation="MPKIKIEQATYDRLAELSGKAGYSSVDEFVTHIIERELEKFQDA
                     SDDQQIEERLRGLGYIE"
     gene            complement(5153..7411)
                     /locus_tag="PLU99_10745"
     CDS             complement(5153..7411)
                     /locus_tag="PLU99_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO17574.1"
                     /translation="MRRFQKTRSYMPFLRFSPRRLRADELLVAAAALIWLAASQFQIA
                     AHWAGIALFEYFGHGEAPLPVAASEPTLAYIGPGAGIALIGSFLTIFVALLSAFAALL
                     VWPLRLIWRLIRGRHAYKYAQVPRVVILGLDGLDPELAEKFMDEGLLPNLQRLRDEGS
                     FRRLGSTWPPLSPVAWSSFSTGANPGKHNVFDFLTRNPADYRPTISSVRLREPRRKFK
                     LGRYVIPLSKPEITLTRKSKPWWKVLSDAGIFSAVLRVPVTFPPDKFHGVQLSAMCVP
                     DLRGSQGIFTHYVETGQEGATMDGDVGGDRILVTRNGRAVESFLRGPVNTLRVDRPEM
                     RLPFRVVSDGNGAAKMRLDGQEIHLPLNKYSEWVRFAFHPAPGIKVRGICQFLLKSFE
                     PPFDLYCTPLHIDPHKPVMPISHPASYATYLAAKHGTFATLGLAEDTWSLSEKVLTED
                     EFLEQTYEIHAEREAMFFDTLRCVRQGAIACVFDTSDRVQHMFFRFFDEKHPALQPQE
                     RASHAAAFRQMYKVMDDLVGRTLEAIGDETALLVMSDHGFKPFRRGVDLNAWLVANGY
                     MVLKDGAKTASHPYLVDVDWSKTRAYAIGLAGIYINKKGREGQGIVAPGQEARDLLRE
                     LSRKLTGLRDDQTGEVAIHEAVLSADVYRGPYIDQGPDLLIGYNVGYRVSWDAAVGKA
                     GTAVFEDNRKAWSGDHCVHPALVPGVLFSNMKLREEPVNIIDIAPTVLELFGLEKPAY
                     MDGKSLLPAETE"
BASE COUNT         1427 a         2539 c         2616 g         1470 t
ORIGIN      
        1 ccctcgacca gaacctggcc cgcctgaatt tcgtcaccag tgggcagatt taccggcggg
       61 tgctcgagcg cgagcgcatc ggcggctatc tcggccgcga cgtacaaatg atcccgcacg
      121 tgaccggcga ggtgaaagac cgcctgcgcg agctggcgat cgcctcgcgg gccgacgtgg
      181 tgttcgtcga aatcggcggc acggtcggcg acgtggagaa cgcgtactac atcgaagcca
      241 tgcgccagct tgctttcgaa gaggggccgc gcagcgtgtg cttcgtcgcc ttgacgtacg
      301 tcatggagcc gtcgatcctc ggcgagcaga agagcaaggc ggcccaactg ggcatccgcc
      361 ggctgctcga atgcgggata cagccgcaca tcatcgcctg ccgcgcccag aacccggtca
      421 cgcacaaggt acgcgagaag atcgccatgt tctcgaacgt gccgcccgac cgtgtcttca
      481 gcatgcacga ctgcgagagc gtctaccaga ttccggaaat gttgcgcggc gccggctacg
      541 accgggcggt aatcgaatgg ctgcggcttg aaaacaaggt cgatgagcag gccgaacgcg
      601 cttcgcgcga ggtttgggga cgctatctgg cgcggctgcg gtcggccgag caccaggtca
      661 cgatcggcat caccggcaag tacaccgcgg ttcgcgacgc gtacgccagc attctgaagg
      721 ccctggagca cgccggcgtg cattgggggt gcaaggttca cgttcgctgg attgatacct
      781 cgcaccttaa cgatcaaacc gtctcggagg cggtcaaagg gctggacggc atcatcgttc
      841 ccggtgcgtt cggcacgcgg ggcgccgagg gcaagatcgc ctgcctcaag tacgcccgca
      901 tgagcaagct gccgtgcctg ggcatctgct acggcttcca aatggccgta atcgagttcg
      961 cccgcaacgt gtgcggcatg gaagacgcga actcgaccga gatcgacacg gagtgccgct
     1021 accccgtcat aaacttgctg cccgagcaga agaagatcga gggcttgggc ggcacaatgc
     1081 ggctgggcgg ccaggacgtt cacgtgcgta agggcacact ggcctggcgc atgttcggcg
     1141 agcgcacccg catgcgcttc cgccaccgct acgaagttga cccgcgctac ataccgcaac
     1201 tggaagaaca cggcctgatc ttctccggcc gctcgccgcg ggcggagatc atgaacatcc
     1261 tggagctgcc gcaggagatg cacccctatt acgtcggcac gcaggcccac cccgagctga
     1321 tcagccggcc attgcgtgcc aacccgatgt tcgccggcct ggtccacgcg gccctgcggc
     1381 gtgcttaccc ggacttcacc gcccctctga ccttcgcggg cttgatggag cgcgaaaacg
     1441 gcgcggagcc cgcagtcggc gaggcggagg tggtcgcggc cgacctggtt gcgcgggagc
     1501 aaagtaaagc ttagcccggt tccgcctgat ccgaggcccc ctgatccaaa cccttgatcc
     1561 gaggccgaag cggcagcgag ggccaccggt ccgtgtccgc gcatgccacc tgagcgattg
     1621 ggagcacgct ggtagattcc ggctcgacac gagccggctc tgctactcac tgcggcggca
     1681 ccactggtcc agccaagcga tgaccgtctc gtgccattga atgctgttgt gcggcttgag
     1741 gacccagtgg ttctcatccg ggaaatacag caatttgctg gggatgccgc ggcgctgcag
     1801 ggctgtgaag gttgagaggc cctgcgtatc cacgacgcgg aagtcctggc cgccatggat
     1861 gaccagcgtc ggtgtgcccc agttctgcac gtgatcgatc gggttgtgct tggtgtattc
     1921 ttccggccgc tcccacgggg gcccgccatg ctcccattcc gggaaccaca attcctcggt
     1981 gtcgtagtag gccatgcgct cgtcgaggtt gccgtcgtgg cacacgaggc agcggaaccg
     2041 gccgggccag acgccgtgca gccagttgat catgtatccg ccgtaagaag cgccgagggc
     2101 ggcgacgcgc tcgccatcca tccatgggta gcgcgccagg gcggcctcca agcccttttg
     2161 caggtcctcg agcggacgat cgccccagtg gccattgata gagtcgacga aggcctgccc
     2221 gtagccgacc gagccgtgga agtcgaccat gaccgcggcg tagccggcgc cggcgtaggc
     2281 ttgcgggttc cagcgatagt ggaagtcgtt gccaaacgag ccttgcgggc cgccgtggat
     2341 gaggaaggcg accgggtatt tcttctgcgg gtcgaagtcg atggggcgga cgatctggca
     2401 gtagacggtt tcgtcgttcc agcctttgaa tgtgaattgc tccggttcgc ccatgcgtgc
     2461 ggcggcgacc ttggcagcgt tgatgtgtgt gatttggcgg agatcggttc cgtcggggtt
     2521 tatggagtac agttcagcgg gctggcgcag gtgatgtagg gcgaagacga ttcgcgagcc
     2581 ggcggcggcg atatcggtga tgtggccttc gaaaagcacg gtgcggacgg cgccggtcgc
     2641 cacatcgacc gcgaacaacg agtgctgggc caggttggcg gcgcaagtgt agatggtcgc
     2701 gccgtcgggc gaccaggcga gctcggcggc ggagcggtcc cattgttcgg ttagtacgcg
     2761 cgcggggggc gctttggcat tttccggggc gacacgcccc ggctctgcta cttgccccgg
     2821 ctctgctact tgcctcgact ctgctactac gatgcgctgg cgatcggatt cgtacttggg
     2881 acgctgcatc gccaggtagg ccaggctctt tccgtcggga gagaagaccg ggcgcgtaat
     2941 gacggcctga ttgcccggag tcagcagccg cgggggcgct gagccgtcga tcggcgccgc
     3001 atacaggtcg aactgggtcg accaggcctc ctcgcgcccc acgttgcggg cggagaagac
     3061 aatttcccgg ccatcgggcg tgaacgtgaa ctcttctgca ccgccgaagg gctttgatgg
     3121 gacgtcggcg tccatgccct tcattacgtc gatcggcgcg ccgccggagt tggtcgggct
     3181 ttgtactacc ggctcaatcg gcaacacgaa caggtgcgcg cgccggccgt ccttccaagc
     3241 gtcccagtgg cgcacgaaga ggcgctcgta gatacggccc gtggacttcc gcttgctgat
     3301 ttcctccagg cgggccttgg tttcgtcgag cgtggcggcg tcgacgaata catcgatgct
     3361 taccgccagg tgccgaccgt caggcgagat cgcgaagcag ccgacatcca ggggcaagtg
     3421 tgtgacctgc tcggcctcgc cgccgtctat cgaaatccgc cagacctggg agccgccgtt
     3481 gcgggcggaa ataaagtaga tcgcgcggct atcgggtgac cagcgcgggt tgctgcaccc
     3541 ggccggatgc gtcgtcaggc gccgcagccc ggttccgtcg attccgacca gccacaagtc
     3601 ggtgcggccc ttgttggcct caagatcggt gacgcggagt gtgaaggcga tgtggcgggc
     3661 gtcgggcgaa acctgcggct cgccgatgcg gtccatcgcc agcatgtcgt ggacggaaaa
     3721 cgggtgtgtg ctgtcatgtt cgcgctgctc agtcgtcatg gcggtctccc atcggccaaa
     3781 acaagtcggc cagcgtgcca cggcggggcg gattctgcaa gcatcaggcg ggcggcgttt
     3841 ggcgggtgag ccggctcaac ggccgtgcga tgcattcttc agaagcgtac tttcaacacc
     3901 ggcttgagca cgaaagccat caggattgag gccaccagga acgtggccca ccaggggatg
     3961 tcgaaaccga aaaccggcgt ttgccgcggc ggataatgca cgatagcggc gcgcaccggc
     4021 gagtcggccg ggaaggccgg ttcgccaggg tgcagcaagc ggtcccagaa gctgacgccc
     4081 ggccgctgca tgctgactgt gcatgggcgc tgcgggtcgt cgcacacggc cagttctttt
     4141 tccacggttt gtccggcgac ctgccagcgc aggcggccgc tggtcgcgcc gtcttgcggg
     4201 ctgatgcgcc agtaaattgc attttcgcgc gcatcacgca gcgcgggcgt ctggatgatc
     4261 acgcctggcg gggtttcgag cgctgcgttt tgcccagtct cccatgcttc cggcgccagc
     4321 ttcagctcga caattgccac ttcacccggg cgtagcggcc ggtactcgaa acgcagcgca
     4381 agctgaacga gcagcagcac aaaaggcacc agcatcgcga cgaaggccgg cagggcgtac
     4441 cagaatcgca gacccgccgc cttcagcagg ccggcctggc tgcggaaagt gacccgcaaa
     4501 tcgtccttga aaagccgcat ggccagcaga ttggccttga tctgatcgcc aacgcgcgcc
     4561 agggccttct gattcgacgt atagcgaaac atgagcccgg ccagtgcgcc cgcgatggcc
     4621 gcccagatca cgagcacgag cacccccggc agtgggcgca gcggatacca cagcagttct
     4681 gttaggttcg tcaagacggc gttgacaagg ttcatttttg aagctgcttc atcgcgccca
     4741 agtcaattct cactttgtac tttgcgcttg tgtttcgggc tcgaatcgtc ttgcccactt
     4801 gccctcgctg gcgcttcggg ctcgggtcat tcacgctcgg atcattcaag ggctcggatc
     4861 attcaagggc tcggatgatt cgatggttcg gatcgttcga gggttcgcat cattcgatat
     4921 accccaagcc gcgcagccgc tcctcgatct gctggtcgtc gctggcatcc tgaaactttt
     4981 ccagctcgcg ttcgatgatg tgcgtcacga actcgtccac cgaagagtag ccggccttgc
     5041 cggagagctc cgccagccgg tcataagtcg cctgttcaat cttgattttt ggcattttca
     5101 tcacctcttc acattggcgc gggcattgcg gcccttgatg cccggtttcg cttcattctg
     5161 tctccgcggg caacaggctc ttgccgtcca tataggcggg cttttccagt ccgaacaact
     5221 cgagcacggt cggggcgatg tcgattatgt tgaccggctc ttcccgcagt ttcatgttgc
     5281 tgaacagaac gcccggcacc agggccggat gcacgcagtg atccccgctc caggccttcc
     5341 ggttgtcttc gaataccgcc gtgccggcct tgccgacggc cgcatcccag gagacgcggt
     5401 agccgacgtt atagccgatc aggaggtccg ggccctggtc gatatagggg ccgcggtaca
     5461 cgtccgcact aaggaccgcc tcgtgtatgg cgacctcgcc cgtctggtcg tcgcgcaggc
     5521 cggtgagttt gcggctcagc tctcggagta aatcgcgcgc ctcctggccc ggtgcgacga
     5581 tgccctggcc ctcgcggccc tttttattga tgtagatgcc cgccaggcca atggcgtacg
     5641 cccgcgtctt gctccagtca acgtcgacca ggtacgggtg cgatgccgtt tttgccccgt
     5701 cctttagaac catgtacccg ttcgcgacca gccaggcgtt caggtccacg ccccgccgga
     5761 acggcttgaa gccgtgatcg gacatgacca gcagggccgt ctcgtcgccg atcgcctcca
     5821 gcgtgcgccc gaccaggtca tccatcacct tgtacatctg gcggaacgcc gccgcgtgtg
     5881 aggcccgctc ttgcggctgc aaggccgggt gcttctcgtc gaagaagcgg aagaacatgt
     5941 gttgcacgcg gtcggacgta tcgaacacgc acgcgatcgc gccctgccgc acgcaacgca
     6001 gcgtgtcgaa gaacatcgcc tcgcgctcgg cgtgaatttc gtaagtctgc tcgaggaact
     6061 cgtcctcagt cagcaccttc tccgaaagcg accacgtgtc ctccgccaaa cccagcgtgg
     6121 cgaacgtccc gtgcttggcc gccaggtagg tcgcataact ggccggatgg gaaatcggca
     6181 tcaccggctt atgcgggtca atgtgcagcg gcgtgcagta caggtcgaag ggcggctcga
     6241 agctcttcaa caggaattgg caaatgccgc gcaccttgat gcccggcgcc gggtgaaacg
     6301 cgaagcgcac ccactcagaa tacttgttaa gcggcaggtg aatctcctgc ccgtccagcc
     6361 gcatcttcgc cgcgccgttt ccgtctgaaa ccacgcggaa gggcaggcgc atctcagggc
     6421 gatccacgcg cagcgtgttc accggcccgc gcaggaagga ttcgacggcc cggccattgc
     6481 gggtcaccag gatgcgatcg ccgccgacgt cgccgtccat cgtcgccccc tcctggccgg
     6541 tttcgacgta gtgcgtaaag atgccctggc tgccgcgcag gtccggcacg cacatggccg
     6601 aaagctgcac accgtggaat ttgtccggcg ggaatgtcac cggcacgcgc agaaccgcgc
     6661 tgaaaatgcc cgcgtcgctc agcactttcc accacggctt gctcttgcgg gtgagcgtaa
     6721 tttccggctt ggaaagcggg atgacgtagc gcccaagctt gaacttgcgc cgcggctcgc
     6781 gcagccgcac cgatgaaatc gtcggccgat aatcggccgg gttgcgcgtc aggaagtcga
     6841 agacgttgtg cttgccgggg ttggcgccgg tgctgaagga ggaccaggcg accggcgaaa
     6901 ggggcggcca ggttgagccc agccggcgga agctgccctc gtcgcgcaag cgctggaggt
     6961 tcggcagcag cccctcgtcc atgaactttt ccgccagctc cggatcaagc ccatccagtc
     7021 ccagaatgac cacgcgcggc acttgggcgt acttatacgc atgccgcccg cggatcagac
     7081 gccagatcag ccgcagcggc cacaccagca gagcggcgaa ggcagaaagc agcgcgacga
     7141 agatggtcag gaaggacccg ataagcgcga tgccggcccc gggtccgata tacgccagcg
     7201 ttggctcact ggccgcgacg ggcagcggcg cctcgccgtg gccgaaatat tcaaagaggg
     7261 cgatccccgc ccaatgcgcc gcaatttgaa actgcgaggc ggccagccag atcagcgccg
     7321 cggcggcaac cagcagttcg tcggcgcgga gtctccgcgg gctaaagcgc aagaatggca
     7381 tgtacgaacg ggtcttctga aaccgcctca acactcactc ccgcgggcgc gtccgtccgc
     7441 atcggctagc gaactccttt ttatatgcgg ggccggccat taggtcgagc gctgtaaccc
     7501 gtgagtttgc agcgttcttc accgagccgg cgtcagacgc acggactcgc ggtgttggct
     7561 tcttccggag ggtgacggct aagcgtgact ggcaagccaa agaaggagcg cgagtattaa
     7621 cgcctggacg atgtctgcgg acatgctgac ctcctttgct gacgcgagcc ctcgtactta
     7681 tcgcaccagg tcagacggcg gctgaatgcg ccattcgccc gtctgctgcc tcactatcgt
     7741 attccgttgc ggccgtcaaa tatttgggcc ggtttgcccg ggagatgggg ctcaggttga
     7801 ggcggtgctg ctgtgccgct gccaccttag cgctgacgta accgttaact tgttcgtcta
     7861 gacccgttcg ctggccctta cgggtcggcc ggttaccgcg ggcgggcaaa gctgaccgct
     7921 ttgaaagcag ccggcgtaaa ttccggccat aaaatcctcc cgcaagccga gtgcagcaaa
     7981 aggggagtgt gtgatggatg ctgcctttga accaagcggc cggccgaacg actggatgga
     8041 cgagccggag cc
//
LOCUS       DAOSYS010000167         8041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7631_length_8041_cov_5.702479, whole genome shotgun sequence.
ACCESSION   DAOSYS010000167 DAOSYS010000000
VERSION     DAOSYS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7631_length_8041_cov_5.702479"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="PLU99_10750"
     CDS             <1..693
                     /locus_tag="PLU99_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17575.1"
                     /translation="QVIAVPRYNQWTLVDPEQRPILKKVVELPAAQRDQLLRSSDARL
                     RGIGIFIAEQQGDLAALLSLKDMLSDNASTVPYAVPVAQVGEYVVRPQTVADYLTTVY
                     QEWFGVDVDKSQERFNSLLAPLSGQPQNFVQPWIVRLRRAASDEKAVAEIKKSIAALP
                     EDVRWAVVTLGYSDSLYSKDEARALLGGLSRPVQERLRSGEELLPNEPLLQSTSFRAA
                     LMRVYGELAVAD"
     gene            961..2373
                     /gene="murC"
                     /locus_tag="PLU99_10755"
     CDS             961..2373
                     /gene="murC"
                     /locus_tag="PLU99_10755"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOO17576.1"
                     /translation="MDDVLPQDLRNFPIHMVGIGGSGMCGLAALLLRRGARVSGTDGR
                     DGDELRRLAAAGATVSTCQAAESVPRGVQMLVKSAAVRDDHPEIVEARRRGVCVVKYA
                     ELLGLLMAHCGEGIAISGTHGKSTTAAWLSYVLRRAGRDPSFVIGAGVRQLGGGSGVG
                     DSPYFVAEACEYDRSFLNLRPRRAAILNIEEDHLDYYADINDITAAFREFAAGIPESG
                     LLVTNADDPHCRAVADARNGRVQLFGRGAAAEWRAVDVTRENGTYAFVGCRGTERLGR
                     VVLTIPGRHNVDNALAVIALAHDCGLSWDAIAPPLAEFCGAQRRLELRGEIGGVRIVD
                     DYAHHPTEIRVTLLAARERYHPRELWCIFQPHQHSRTRFLMGHFAQSFSQADHVIVPD
                     IYFVRDSQRDRELVCAADLVEQIRARGAAAEYLPDFGGIVERVAKDVQPGDVVMTMGA
                     GNVWMVADELLARLRSDLPG"
     gene            2333..3226
                     /gene="murB"
                     /locus_tag="PLU99_10760"
     CDS             2333..3226
                     /gene="murB"
                     /locus_tag="PLU99_10760"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOO17577.1"
                     /translation="MSCLPGCEAICQVDAPLSEHTWYKLGGRARFLLTPRDEAELATV
                     LECCRRQGVSWRVLGRGANVLVRDTGVDGAVIKLVGPAWELVLWDGAVVTAAAGADFP
                     RLAKQSVERGLGGLDGLAGIPGSVGGIVRMNAGGKYGNISQVTRAVRVMDAGGRIETW
                     TPERIGFGYRHTEINNVIVLDATFVLQPGDRDSLRERFQAIWKEKAGQQPAVSGRTAG
                     CIFKNPLVDGQRLPAGRLIDQAGLKGARAGGAEISRKHANFIEAGQGAKAQDVIDLME
                     LARQTVRRSAGIELEPEIEVW"
     gene            3274..4197
                     /locus_tag="PLU99_10765"
     CDS             3274..4197
                     /locus_tag="PLU99_10765"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOO17578.1"
                     /translation="MSTEQDLAGTALRITVLRGGPSAEREISLASGKAVAEALRYRGH
                     DVFEADIGPGDLSPLEHPADVVFPALHGTFGEDGQLQRILEQRGMRFVGSGSAASALA
                     MDKVASKQRVLEIGLETPPFEVWTAVDSYESTRLPLPVVVKPADQGSSVATFIVREAE
                     QFAPAVSEVVGTFGRALVERFIVGDEITVGILGGEPLPPICVRPRRAFYDYHAKYLDD
                     ATEYLFDAGHTAALLERVQVASRRIFHALGCRHLGRVDWIADADDQLWFLEVNTIPGF
                     TTHSLVPKAAARVGIPFDELVERLVYMARRS"
     gene            4194..5096
                     /locus_tag="PLU99_10770"
     CDS             4194..5096
                     /locus_tag="PLU99_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17579.1"
                     /translation="MRHTRRNKPIPRDRGLRPPSISPRSARRLALALLWAGTFVGLAY
                     GLHRLDAFAAEAADGAPWTPQFPNVPGTIPDWIPIEVADAVGLCEGGALCAMSINDPL
                     LCEAIGTAVRSSPWVAKVERVTKQPGAVNIYATFRTFLTFVVYKGKGYLVDEEGYRLP
                     REETESRLPAYEMILLEGVQEPPPPYGQAWEGEAVKGGLQLVKLLQNDCPPGPRAFIK
                     AVDVSNYGGRKNPRDGWLSIRTVHARTKIQWGYPPGEEYLTEASAQKKLAYIQLAFSR
                     YGEFPDGKIFDVRDRRDLQVSNAP"
     gene            5123..5944
                     /locus_tag="PLU99_10775"
     CDS             5123..5944
                     /locus_tag="PLU99_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17580.1"
                     /translation="MNELLNMAYDAAMQHIPHSELWKPQAGLALVGAFGLILLLRGAK
                     LFPAITALAFMAGGGIAGGFLAKSLGTALSPTVVVTGVIGFGLGYFLFKLWLAALVGL
                     SLAAVGLMLYGEKVVRPYVENFSSRNLDAGQGLMGINIPETPLATQAPSSWAAQSWDH
                     LSTVVPNFQASFWAIVGSIGLAGLVLGLLLPKVSRALVAATLGTLLSCLALLLILKSK
                     WPAALSELQRLGEWGWTIVALLWAVSLLYNFTSLRRKPRPAATSEEPETPEPATA"
     gene            6101..6718
                     /locus_tag="PLU99_10780"
     CDS             6101..6718
                     /locus_tag="PLU99_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17581.1"
                     /translation="MSGIQIETFRWAAAQPALTGLVIMGAGFIYGFYGARLFRLLIAA
                     SCAGFGWLGGHLAAGFVSDIPAPVLGAIGAVGLGLAATRMLRAGVAFTSLATWAIIGA
                     YLAGQFGLEREGGLIAAGLAGLFGLTHALLDCRGTTLVLTTLQGAVMIVVGWVSVTSQ
                     ITPSLGATFREWANSQELLVPGFLAMLFATAYSCQVLRQRNIKGI"
     gene            complement(6795..7103)
                     /gene="gatC"
                     /locus_tag="PLU99_10785"
     CDS             complement(6795..7103)
                     /gene="gatC"
                     /locus_tag="PLU99_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOO17582.1"
                     /translation="MPAQINEEHVRRIARLSRLKLTEDEVALFAGQLGRIVDYVEQLR
                     EVDTTGVEPMAHPLPVTNVAREDEPHVPFDNERALANAPQRADGFFKVPPVLDHSGGA
                     "
     gene            complement(7160..7429)
                     /gene="rpmB"
                     /locus_tag="PLU99_10790"
     CDS             complement(7160..7429)
                     /gene="rpmB"
                     /locus_tag="PLU99_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOO17583.1"
                     /translation="MSRVCKFTGKRTTFGNSVARRGKAKYLGGVGRKTTGISRRKFKP
                     NIQKVRAVIDGKVTRVRVSAKAIRMGLITKPLKRDWQKPEAAAAE"
BASE COUNT         1380 a         2402 c         2788 g         1471 t
ORIGIN      
        1 caggtaatcg ccgttccgcg gtacaaccag tggacattgg tggacccgga gcagcgcccg
       61 attctgaaga aggtcgtcga attgcctgcg gcgcagcggg atcagctttt gcgcagcagc
      121 gatgcccggc tgcgcgggat cggcattttc attgccgagc agcagggcga cctggctgcg
      181 ctcctgtcgt tgaaggacat gctttccgac aatgcatcca cggtgccgta cgccgtgccc
      241 gtggctcagg tgggcgagta cgtggttcgc ccgcagaccg tggccgatta cctgaccact
      301 gtgtaccagg aatggttcgg ggtggacgtg gacaaatcgc aggaacgttt caacagcctg
      361 ctcgcaccgc tgagtggtca gccgcagaac ttcgtgcagc cctggattgt ccggctgcgg
      421 cgcgcggcgt cggacgaaaa ggccgtggcg gagatcaaga agagcatagc ggcgctgccg
      481 gaagacgtgc gttgggcggt ggtcacgctg ggctatagcg acagtcttta cagcaaagat
      541 gaggcccgcg cgttgctggg cgggctatcc cggccggtcc aggagcgctt gcgctccggc
      601 gaggagctgt tgccgaatga gccgttgctg cagagtacgt cgttccgggc ggcgctgatg
      661 cgggtttacg gcgagctggc agtggcagac tgaagtctgc cgctggtaag cggcagcact
      721 cacgggttcg cagtgcgcgc tgtagctgaa acggtggcgg cgcgcgcatt cgggagcggc
      781 gcgtgatcgc acattccgtg gcgacacgcc ccggctctgc caaaggcggc gcgccccggg
      841 tctgcgttgt ggttttggcc gccaagtagt atgctgtccg aagccggcag ggatgctgcg
      901 agctatttct ggcggtcgaa catggatgga agcgcggcag cggtgatcga aactccaagg
      961 atggatgacg tgctcccgca ggacctgcga aatttcccga ttcacatggt aggtatcggc
     1021 ggcagcggca tgtgcggcct ggctgcgctt ttgctgcgcc gcggcgcgcg cgtcagcggc
     1081 accgatggac gggatggcga cgaattgcgg cgcctggccg ccgctggagc cacagtcagc
     1141 acgtgccagg cagcggagag cgtaccgcgc ggcgtgcaga tgctcgtcaa atccgcagcc
     1201 gtgcgcgacg atcacccgga gattgtcgaa gcccggcggc gcggcgtgtg cgtcgtgaag
     1261 tatgccgagc tgctagggct gctcatggcc cattgcggtg agggcatcgc catcagcggc
     1321 acgcacggca agagcaccac ggccgcctgg ctctcgtatg tcttgcgccg cgcgggccgc
     1381 gacccgagct ttgtgatcgg cgccggcgtc aggcagctcg gcggcggcag cggcgtgggc
     1441 gacagcccgt atttcgtggc cgaggcctgc gagtacgacc gttcgtttct taacctgcgt
     1501 ccccggcggg ccgccattct aaatatcgaa gaagaccacc tcgactacta cgcggacatc
     1561 aacgacataa cggcagcttt ccgcgagttc gccgccggca ttcccgagag cggcttgctc
     1621 gtaacgaatg ccgacgatcc acactgccgc gccgtggctg atgcacgtaa cgggcgggtc
     1681 cagctattcg ggcgcggggc ggctgccgag tggcgcgcgg tcgacgttac tcgcgaaaac
     1741 ggcacttacg ccttcgtggg ctgccgcggc acagagcggc tggggcgggt ggtgctcacc
     1801 attcccggcc ggcacaacgt ggacaacgcc ctggcggtga tcgcgctggc gcacgactgc
     1861 ggcctgagtt gggacgccat cgcgccgcct ttggccgaat tctgcggggc acagcggcgg
     1921 ctggagctgc gcggggaaat cggcggcgtg cgcattgtgg acgactacgc ccatcatcca
     1981 accgagatcc gcgtgacgct gctggcggcc cgcgagcgct accacccgcg cgagctgtgg
     2041 tgcatcttcc agccgcacca gcattcgcgt acgcgttttt tgatggggca cttcgcccag
     2101 agcttcagcc aggccgatca cgtgatcgtg ccggacatct acttcgtgcg tgacagtcag
     2161 cgtgatcgcg agttggtgtg tgcggccgat ctcgtcgagc agatccgcgc ccgcggggcg
     2221 gcggcggagt acctgcccga tttcggcggc atcgtggaac gggtcgcgaa agacgtgcag
     2281 cccggggacg tggtgatgac gatgggggcc gggaacgtct ggatggttgc cgatgagctg
     2341 cttgcccggt tgcgaagcga tttgccaggt tgatgcgccg ctgagcgagc acacctggta
     2401 caaattggga gggcgggccc ggttcttgct cacgccgcgg gatgaggcgg aattggcgac
     2461 ggtgctggag tgctgtcgca ggcagggcgt cagttggcgc gtgctggggc gcggtgcgaa
     2521 cgtattggtg cgcgacacgg gggtggacgg cgcggtcatc aagctggtcg ggccggcctg
     2581 ggaactagtg ctctgggacg gagcagttgt taccgccgca gccggcgccg attttccccg
     2641 actcgccaag caaagcgtgg agcgcgggtt gggcggtttg gacgggctgg cgggcattcc
     2701 gggcagcgtg ggcgggatag tgcgcatgaa tgcgggcggc aagtacggca acatcagcca
     2761 ggtcacgcgg gccgtccgcg tcatggatgc cggcggtcgc atcgagacct ggacgcccga
     2821 gcgtatcggc ttcggatacc ggcatacaga aatcaacaat gtaatcgtgc tggacgccac
     2881 gttcgtcctg cagccgggcg accgcgactc gctgcgcgag cgcttccagg ccatctggaa
     2941 ggagaaagcc ggccagcagc cggccgtcag cgggcgaacc gcgggttgca tctttaagaa
     3001 cccgctggta gacgggcagc gcctgcccgc cggccgcctg attgaccagg ccggcctgaa
     3061 aggggcgcgc gccggaggcg cggaaatcag ccgcaaacac gcgaatttca ttgaagccgg
     3121 acagggcgca aaggcacaag acgtgataga cttgatggag ctggcccgtc agacagtgcg
     3181 gcgcagcgcg ggaatagagt tagaacccga aatcgaagtt tggtgagccg gcgcccgccg
     3241 acagggagta aggcactgac cagagcatta accgtgagta ctgagcaaga tttggcaggc
     3301 acagcgttgc gcatcaccgt tctgcgcggc ggaccaagcg ccgagcgcga gatcagcctc
     3361 gcgtcgggga aggcggtggc cgaagcgctt cgctatcgcg gacatgacgt ttttgaggcc
     3421 gacattggtc caggggactt gtcgccgctc gaacacccgg cggacgtggt ttttcccgcc
     3481 ttgcacggca cgttcggcga ggatgggcaa ttgcagcgga ttctcgagca acgcggcatg
     3541 cgcttcgtgg gctcgggctc ggcggcgtcc gcgctggcca tggacaaggt cgccagcaag
     3601 caacgcgtcc tcgagatcgg actcgaaacg ccgccctttg aggtctggac cgctgttgac
     3661 agttacgaaa gcacgcgcct gcccctgccc gtggtcgtca agcccgccga ccagggcagc
     3721 agcgtagcca cgttcatcgt gcgcgaggcc gagcaattcg caccggctgt ctctgaggtc
     3781 gtcggcactt ttgggcgcgc gctggtcgag cgtttcatcg taggcgacga gataaccgtc
     3841 ggcatcctgg gcggtgaacc gttgccgccg atttgcgttc gcccgcgccg cgcgttctat
     3901 gactatcatg ccaaatacct cgacgacgcg accgaatacc tgttcgatgc cgggcatacc
     3961 gccgcgctgc tggagcgcgt gcaggtcgcc agccggcgca tcttccacgc cctgggctgc
     4021 cggcatctcg gccgcgtcga ctggatcgcg gatgctgacg accagctctg gtttttggag
     4081 gtgaacacga ttcccggttt caccacccat tcgctggtgc ctaaggccgc ggcgcgcgtc
     4141 ggaatcccct tcgacgagtt ggtcgagcgg ctcgtctaca tggcgcggcg ctcatgaggc
     4201 atacgcggcg gaacaagccg attccgcgcg accggggctt gcgcccgcca agcatctcgc
     4261 cgcgcagcgc gcggcgcctg gccttggccc tgctatgggc cgggacgttt gtagggctgg
     4321 cgtacgggtt gcaccggctg gacgcgttcg ccgcggaagc ggcggacggc gcgccctgga
     4381 cgccgcaatt cccgaacgtt ccaggcacga ttcctgactg gatacccatc gaggtcgcag
     4441 atgccgtggg gctctgcgag ggcggcgcgt tgtgcgccat gagtatcaac gatccgctct
     4501 tgtgcgaagc gattgggaca gccgtgcgaa gctcgccctg ggtcgcgaag gtcgaacggg
     4561 tcacaaaaca gcctggcgcc gtaaacatct atgccacttt cagaactttt ctgaccttcg
     4621 tggtatacaa gggcaagggt tatctggtgg acgaagaggg ttatcgcctg ccgcgtgaag
     4681 aaaccgaaag ccgtcttccc gcctacgaga tgatcctcct tgaaggcgta caggagccgc
     4741 cgccgccgta cggccaagcg tgggaaggcg aagcggttaa aggcggcctg caactcgtaa
     4801 aactgttgca aaacgattgc ccgccgggcc cgcgcgcgtt catcaaagcg gtagatgttt
     4861 cgaactatgg cgggcgcaag aacccgcgtg acggctggct tagcatccgc accgtgcacg
     4921 cccgcaccaa aatccaatgg ggatacccgc ccggcgaaga gtatctaacg gaagcgtcgg
     4981 cccagaagaa gctggcctac atccaactgg cgttttcgcg gtacggcgag ttcccggatg
     5041 gtaagatctt cgatgttaga gatcgcaggg atttgcaggt gtccaacgca ccctgagttt
     5101 tggaggtcca tggagccggc gcatgaacga actgcttaac atggcctatg acgcggccat
     5161 gcagcacatt ccgcattccg agctctggaa accccaggcc ggcttggctc tcgtgggcgc
     5221 gtttgggctc atcctgctgc tcaggggcgc caaactgttt ccggccatta cggctttggc
     5281 tttcatggcc ggcggcggca tcgctggcgg gttcctcgcg aagagtttag gaacggcgct
     5341 ctcgccgacg gtggttgtca caggcgtaat cggtttcggg ctgggttatt tcctctttaa
     5401 gctctggctg gcagcgctgg tcggtcttag cctggccgcg gtcgggctga tgctctacgg
     5461 cgaaaaagtc gtaaggcctt acgtcgagaa cttctcttcg cgcaacttgg acgccgggca
     5521 agggttaatg ggtatcaaca ttcccgaaac accactggct acgcaagccc cgtcatcctg
     5581 ggccgcgcag tcttgggatc acctgtccac ggtcgtgccc aattttcagg cgagcttctg
     5641 ggcgatcgtc ggctcgatcg ggttggcggg tttggtgctt gggctgctgc tgcccaaagt
     5701 ctcccgcgca ctggtcgctg cgacgctggg caccctgctg agttgcttgg ctcttttgct
     5761 catactgaag agcaagtggc cggccgcctt gagcgagctc caacgcctcg gtgaatgggg
     5821 ctggaccatt gtggcgctgc tctgggcggt gtccctgctc tacaacttca cgagcctgcg
     5881 ccgtaaaccg cgccccgccg ccactagcga ggaacccgag acgccggaac cagccacagc
     5941 ctagccaccc ataaaagttg ccggccgtca ccccgcaact gtcagccgtg catccgggcc
     6001 gacagccacc ggactgataa ttgataactg aaaagtggcg cctcatattt gccgaacagc
     6061 gcgcgcgttt ccaacgatta acgctgatgg agatacgcgc ttgtccggca ttcagattga
     6121 gacatttcga tgggcggccg cacagccggc tttgaccggc ttggtgatta tgggggccgg
     6181 gttcatctac ggtttttatg gggcgcggct gttccggtta cttatcgcgg cgagctgcgc
     6241 cggcttcggc tggctgggtg gccacctggc cgccggtttc gttagcgaca ttccggcgcc
     6301 ggtgctgggc gccatcggcg ccgtggggct cggtctggcc gccacgcgca tgctgcgggc
     6361 cggtgtagcg ttcacctcgc tcgccacctg ggccataatc ggcgcctatc tggccgggca
     6421 gttcgggctc gagcgcgagg gcggcctgat cgcggccgga ctggcagggt tgttcgggtt
     6481 gacgcacgcg ctgctggatt gccgcggcac gaccctggtg ctgacgacgc tgcagggtgc
     6541 ggtcatgatc gtcgtcggct gggtgagcgt caccagccag atcacgccgt cgcttggcgc
     6601 cacgtttcgc gaatgggcca acagccagga gctgctggtg cccggattcc tggcgatgct
     6661 ttttgccacc gcctactcgt gccaggtgct gcgccagcga aacatcaagg gcatctgaag
     6721 gctaagtagg ctaagttgcc gttttcactt gtgattatca gcagcggcca gcgcctgaaa
     6781 gctgcccacc attctcaggc gccgcccgag tgatccaaga cgggcgggac tttgaagaac
     6841 ccgtcagcgc gttggggggc gttggccagg gcccgttcgt tgtcgaatgg gacgtgcggc
     6901 tcatcctcgc gtgcgacgtt tgtgaccggc aacggatggg ccatcggttc cacccccgtg
     6961 gtatcgacct cccgtaactg ttcgacgtaa tcgacgatcc gccccagttg cccggcgaac
     7021 agcgcaacct cgtcctcggt cagcttcagc cgggagaggc gggcgatgcg gcgaacgtgc
     7081 tcttcgttga tctgggccgg catgatcctg cctgactcgc cccgcttggg agcctcggct
     7141 cagcaaggcc cgcgccgggc tattcagccg cagcggcctc gggcttttgc cagtcgcgct
     7201 tgagcggctt ggtaatcagc cccatgcgga tggccttggc gctgacgcga acccgcgtga
     7261 ctttgccgtc gatgaccgcg cgcaccttct ggatgttcgg cttgaacttc cgccggctga
     7321 ttccggtggt tttccggccg acgccgccca ggtacttggc cttgccgcgg cgcgcgacgg
     7381 agttgccgaa agtggtcctc ttaccggtaa acttacaaac ccgggacatc gcgtgccctc
     7441 gtcttcaaat tcaaaccgca gcgtagctcc acagcgccgc tgccggcagc cgtaacgcgc
     7501 gagttcagca ctttatcacg ccgggcgggg gcggtcaacg ggagagggat aaagggatcc
     7561 agggatcgag ggatcaaggg atctagggat cgagaccggc gagcagagcc ggggcgaggc
     7621 gcccgggcgg gtcgagaggg atcggggttg gagggatcaa gggatctagg gatcgaggga
     7681 gcaagggggc aagagggcaa agtatccgag cccgaagggc tagcgagggc aaccggatcc
     7741 cccgctccga ggatcgaggg atccagggat cgagggggca agggggcaag agggcaagga
     7801 gtctgagcaa acggcgcttt tggtcgcggc ggggaggggg tctgataata ggccccgtac
     7861 ttgggttgga tgggcagttt gccagattgc caagcgggtg ccgctttggc ggataaaagc
     7921 ggcgttttga ttgcgccagt ggcagtttgc cagtttgcca aggtacgcgc gctcgatttt
     7981 ggcaaagtgc cgcgctgcgc gagagcagga gacaggtgaa aggtgaacag gagaggggga
     8041 g
//
LOCUS       DAOSYS010000168         7917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7805_length_7917_cov_5.089545, whole genome shotgun sequence.
ACCESSION   DAOSYS010000168 DAOSYS010000000
VERSION     DAOSYS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7805_length_7917_cov_5.089545"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLU99_10795"
     CDS             <1..760
                     /locus_tag="PLU99_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17584.1"
                     /translation="PEPHVDFFHNAQHVAHGVVESADHLFWYARLIEGGANGGGDRAE
                     QPPSIAVGDSAIIRTRADIAARRFPARVFEISADGALINAGESDELSVGQTGGLYRGG
                     EKIGEVRITRLQRSYAVIQPAETPPEPTATRPASQQPSSGATSAIQPAVLPAIGDVVR
                     FSDPPAPAVHLAEIENVVDGNLFSARALAHVQVTALTPLLVRNFDGGGGVALVLASDG
                     QRICGLVLECSLAAPLRPGAIIVESNPAQPAEQR"
     gene            924..1376
                     /locus_tag="PLU99_10800"
     CDS             924..1376
                     /locus_tag="PLU99_10800"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOO17585.1"
                     /translation="MPDPIRTCDVEIRVRYAEVDAMGYVHHARYFVYFEIGRTELLRL
                     NGIRYRDLEARQLYYVVARLECRFKAPARYDDLLTLTTSTERLSPVRVDHSYRLRRGE
                     SLLAEAHSTLALVGPDGRPTGLPDDIYAALTGSQATDRDGLAPAERVE"
     gene            1634..2662
                     /locus_tag="PLU99_10805"
     CDS             1634..2662
                     /locus_tag="PLU99_10805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO17586.1"
                     /translation="MPSVSEAAASQPANNESAPAKADNESAPASQEAPRRPELASRPA
                     REPQVVEFEITSSEDEPWGRILIELYPERTPVTARTFVQYVNSGFYDGTIFHRIIPGF
                     IVQGGGYTSLTEKKTEGLGDAIRNESRRGTLRNSRGMVAMARKQDPHSAVSQFFINLG
                     DNEQLDYPRAGAYGYCVFGKVIEGMDVVDRIAEVETRVSDAAQRRYERYMQEGRPVEK
                     AEQSEPLSPPILKRARLLEQSELPASVLASPANRRAQPAVEPRPQTRPAAEDEEQATE
                     ESGIEEPALEPAEVEPGDESVPAPESAPAIDGGESEAEQDESTDENRGGAARMKRAIP
                     KRALGSKS"
     gene            2706..3242
                     /locus_tag="PLU99_10810"
     CDS             2706..3242
                     /locus_tag="PLU99_10810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO17587.1"
                     /translation="MPNTSTNPRVELEITCQGDDWGRVVLELDVEKTPQTVQNFLAYV
                     DEGFYDGTVFHRVIPTFMIQGGGYGPDYRPVRKGLRKPIQNEAASGGSNVRGAIAMAR
                     TDDPHSATSQFFISVTDNPFLDYPGHDGWGYCVFGRVTEGMDVVDRIKDVKTRNNPAM
                     GEASQPIDAPVIKSARRL"
     gene            complement(3371..4615)
                     /locus_tag="PLU99_10815"
     CDS             complement(3371..4615)
                     /locus_tag="PLU99_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOO17588.1"
                     /translation="MRAAQWGVILSGMLLAWGGLLGCRNPEVSSSPMFEQARHARQPV
                     SRSAPRPLPQVERAVWVARFHYRYPDDVRTIIRNCADMGVNTVLWQVRGAGTVAYPSR
                     LEPWLEEFGFTSPGFDPLALAVEEAHRRGLRIEAWVNILPGWRGPKPPPLRDQLWNAR
                     PEWFLQDSRGQRQPLGDFYLILNPCLPEVRQHIGAIIDEIVSNYDVDGIHMDYVRYAW
                     DTTPGAEKRYPRDERTLSIYWNETGKRPEDDSASWKHWRANQLTRLVADIRNIVNCRR
                     PGATLTAAVFSDPHDAYNQFFQNGVGWLRAGMVDALMPMAYTDKLGEFEKDVEAYRQL
                     GGRGRIIPGLGIYKHATAAPMRQQLAYCVDCGGDWALFSYGSLWPTHEDRLLKKGPSA
                     KEQAARAIHRDVLGEFTGRDFQ"
     gene            4796..5728
                     /gene="dapF"
                     /locus_tag="PLU99_10820"
     CDS             4796..5728
                     /gene="dapF"
                     /locus_tag="PLU99_10820"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOO17589.1"
                     /translation="MPVKFTKMHGLGNDYVLVSLFEQHVDDPPGLARAISDRHRGVGS
                     DGLILLAPPDSPEADARMIMFNSDGSRAQMCGNGIRCLAKLAWERGLVRRNPMRIATD
                     AGVLSIDLTLDSAGKATQARVDMGAPILQPGRIPVRLAGERVVAAPLAVGTRSLLVTC
                     VSMGNPHAVFFTDRLEDVPLADWGPRIERHALFPEKTNVHFAHVLARGRVRMITWERG
                     AGLTQACGTGACAVGVAAVLNNLTDRMLTVHLPGGDLQVEWVAAEPVRGGAGSPANPA
                     ATAGPGQALAPPERSNHVFMTGPAEEVFTGDWPD"
     gene            complement(5751..6317)
                     /gene="frr"
                     /locus_tag="PLU99_10825"
     CDS             complement(5751..6317)
                     /gene="frr"
                     /locus_tag="PLU99_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOO17590.1"
                     /translation="MTVNDIVKKAQDEMNKAVEFLKQEFRSVRTGRATPALVDTLRID
                     VESYDSSMSLKELANIAVTEGNVIIIKPFDPSTLKDIQRGIEKSGLGINPQNDGKMIR
                     LPVPPLSGERRNQLTNHIKQLAEQQKVAVRTSRRDANKSFDAAKKAKTLSEDDAERGE
                     EQVQKITDEFIKKIDKMTEEKAKEIMEV"
     gene            6580..7572
                     /locus_tag="PLU99_10830"
     CDS             6580..7572
                     /locus_tag="PLU99_10830"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO17591.1"
                     /translation="MQTIFVTGAEGFAGNLLTQQLRQSGYQVWAGVRNRARKLAHERQ
                     QGQAIVCDVSDAINLARAIASAKPDAVVHLAGFSHPHAASEMPLEAYQSIVTAWANVL
                     DAVRRITPRARVLLVSACDVYGRAGADGQPLREDTPPQPVSTFGKLKLTAESIAHTFY
                     ERYHLNLTIARTFHYTGAGQPDSFFFGSIAHRLASWDPAVQGDTLALPDLQCRRDLLH
                     VRDVVSAYERLLQDGKPNEIYNVCSGQAHTCGEIVQMMVQALRTPLQITEQCPAEGND
                     EQVPVFLGDNTKIRTELGWSPTQTFEGAVQELIRSYQRRPTAQAVAHESADSAG"
BASE COUNT         1486 a         2601 c         2441 g         1389 t
ORIGIN      
        1 gcccgagccg cacgttgatt tctttcacaa cgcgcaacac gtcgcccacg gcgtcgtcga
       61 gtcggcagac catctgttct ggtatgctcg gctgatcgaa ggcggtgcaa acggtggagg
      121 cgatcgcgcc gagcagccgc cgtcgatcgc ggtcggcgat tcggcgatca tccgtacgcg
      181 ggccgacatc gccgcgcgcc gtttccccgc ccgcgtgttt gaaatttcgg ccgacggcgc
      241 cctgataaac gccggcgaat ccgacgaact cagcgtcggg caaacgggcg gcctctatcg
      301 cggcggggag aagatcggcg aagtccggat cacccgcctg cagcgcagct atgcggtaat
      361 acagccggca gaaacgccgc ccgagcccac agcgacgcgc ccggccagcc agcagccatc
      421 gtccggagcg acatccgcca tacaaccggc ggtcttgccg gcgattggcg acgtagtgcg
      481 gttcagcgac ccgcccgcgc cggcagtgca tctggccgaa atcgagaacg tggtggacgg
      541 gaatctgttt tccgcccgcg cgctggcgca tgtccaggta accgctctca cgccgctgct
      601 ggttcgtaac tttgacggcg gtggcggagt agcgctggtt ctggcctcag atggacagcg
      661 catttgcgga ctggtcctgg aatgttcgct ggccgcgccg ctgcgcccgg gggcaatcat
      721 tgtggaatca aatccggcac agccggccga acagcgttga actcttcgcg gctatgcagc
      781 gcaaatcctt ggatgcggga gcgcgagcgg acagcgctgc cctgcgtcgc tgtggctaca
      841 atgacgcgcg cggcgccaaa cgcccgccgg acctgtccga cgttggggcg ttcgcgcctc
      901 tgctgtgccg aaaggagagc ctcgtgccag accccatccg cacttgtgat gtcgaaattc
      961 gcgttcggta cgccgaggtg gacgcgatgg gctacgtcca tcatgcccgc tatttcgtgt
     1021 acttcgagat cgggcggacc gagctgctgc gccttaacgg catccgctat cgcgacctgg
     1081 aagcccgcca gttgtactac gttgtggcgc ggctggaatg ccgcttcaag gccccggcgc
     1141 ggtacgacga cctgctcacg ctgacgacct ccaccgagcg gctgtccccg gtccgcgtcg
     1201 accatagtta tcggctgcgg cgcggtgagt cattgctggc cgaagcgcat tccacgttgg
     1261 cgctcgtcgg acctgatggg cggcccacgg ggctgccgga cgacatctac gccgccttga
     1321 ccgggtcgca ggcaactgac cgggacgggc tggcacccgc ggagcgggtc gaataaattg
     1381 cctgcctgcg gccggtttcg accgtcggcg gcagcggagt tctgaaatgc tacgttttcg
     1441 ggtaatcgcg gtttgcgtgt tcgttgctat gggtggtttg gtggcggccc actcggcgtc
     1501 gcagcgtccc acgaaagcgg ggccacgaaa accgaacacg ccgccacagg cttctgagcc
     1561 ggccatgacg acagtgcatg agggccctgc caacaaccgg ccaaagcgtc cccctcaggt
     1621 aggccgccgt ccgatgccct ccgtttccga agcggccgct tcacaaccag ccaataatga
     1681 gtccgccccc gccaaagccg acaatgagtc cgccccggcc agccaggaag ctccgcgccg
     1741 cccggaactt gcatcgcgtc cggcgcgcga gccccaggta gtcgagtttg agattacctc
     1801 gtcagaggat gaaccatggg gccggatact gatcgagttg tatcctgagc gtacgccggt
     1861 cacggcccgg actttcgttc agtacgtcaa tagcgggttt tatgacggga cgattttcca
     1921 ccgcatcatc cccggcttca tcgttcaggg cggcggctat accagtttga cggagaagaa
     1981 aacggagggg ctgggcgacg caattcgcaa cgaaagccgg cgcggcacgc tgcggaactc
     2041 gcgcggcatg gttgcaatgg cccgcaaaca ggacccgcat agcgcggtat cgcagttctt
     2101 cataaacctg ggcgacaacg aacaactcga ctatccgcgc gcgggggcgt acggctactg
     2161 cgtctttggc aaagttattg aaggcatgga cgtagtggac cgcatcgccg aggttgagac
     2221 ccgagtcagc gacgcggcgc agcgacgcta cgagcgctac atgcaggagg ggcgcccggt
     2281 cgagaaggct gagcagtcgg agccgctgag ccctccgatc ctcaagcgcg cccggctact
     2341 cgaacaatct gaactaccgg catccgtcct cgccagcccc gccaaccggc gtgcgcagcc
     2401 cgcggtggag ccgcgtccgc agacccgccc ggccgcggag gacgaggaac aagcgactga
     2461 agagagcggc attgaggagc ctgcgctcga gccggcggaa gttgaaccgg gcgatgaatc
     2521 cgtgcccgca cccgagtctg caccggcaat cgacggcggc gagtcggagg cggagcagga
     2581 cgagtctacg gatgagaatc gcggcggcgc ggcccgcatg aagcgggcga ttccgaagcg
     2641 cgccctgggc tcaaaatctt gacaattgga acccctacct ctttcagtcc acagactgga
     2701 gcatcatgcc aaatacgtcg actaacccac gcgttgaact ggaaatcacc tgccaaggcg
     2761 acgactgggg ccgtgtcgtc ctggaattgg acgtcgagaa gacgccgcaa accgtccaga
     2821 acttcctcgc ctacgtggac gagggcttct atgacggcac agttttccat cgcgtgattc
     2881 cgacctttat gatccagggc ggtggatacg gaccggatta cagaccggtc cgcaagggcc
     2941 ttcgtaagcc catccagaac gaggcggcca gcggtggctc caacgtacgc ggggcaatcg
     3001 cgatggcccg gacggacgac ccgcattcgg caacctcgca gttcttcatc tccgtgactg
     3061 acaatccatt cctggactat ccaggacacg acgggtgggg ttactgcgtg ttcggccgcg
     3121 tgactgaagg catggacgtc gtggatcgaa tcaaggacgt taagacgcgg aataacccgg
     3181 ccatggggga agcctcgcag ccgattgatg cgccggtcat caagagtgcg cggcgcttgt
     3241 aatcgagcag agccgggccg tgccggcccg gactatgccc gtgcgcatct acattgggtg
     3301 ccacgctccc catcgcgtgc cgtgcccgtc cacatcgcgt gccgtgcgcg ccccgtcgcg
     3361 tgccgtatgc ttattgaaaa tcgcgccccg tgaactcgcc cagcacgtcc cggtgaatgg
     3421 cccgggccgc ctgctccttc gcgcttggcc ctttcttcag caagcggtcc tcgtgcgtcg
     3481 gccatagcga tccgtaggag aacagcgccc agtcaccgcc gcaatccacg cagtacgcga
     3541 gctgctgtcg catcggcgcc gcagtcgcgt gcttgtaaat gcccaggccc ggaatgattc
     3601 gcccgcgccc gccaagttgc cggtaggcct cgacgtcctt ctcgaattcg cccagcttgt
     3661 ccgtgtaggc catcggcatt aaggcgtcca ccatgcctgc ccgtagccag cccaccccat
     3721 tctggaaaaa ctggttgtag gcgtcgtgcg ggtcgctgaa gacggccgcc gtcagcgtgg
     3781 ccccgggccg gcggcagttg acgatgttgc gaatgtcggc caccagccgt gtgagctggt
     3841 tggcgcgcca gtgcttccag ctcgccgagt cgtcctcggg gcgtttgccg gtctcgttcc
     3901 aatagatcga cagcgtccgc tcatcgcgtg ggtagcgttt ctccgccccc ggtgtggtgt
     3961 cccaggcgta gcgcacgtag tccatgtgga tgccatccac gtcgtagttg gagacgatct
     4021 cgtcgatgat tgccccaata tgctgccgca cctccggcaa gcacgggtta aggatcaggt
     4081 aaaaatcgcc cagcggctgc cgctgcccac gcgagtcctg tagaaaccac tccggccggg
     4141 cgttccagag ctggtcgcgc agcggcggcg gcttggggcc cctccagccc ggcagaatgt
     4201 tgacccaagc ctcaatccgc agcccgcggc gatgcgcctc ctctaccgcc agcgccagcg
     4261 gatcgaatcc gggcgaggtg aagccaaact cctctaacca gggttcgagc cgggatgggt
     4321 atgccaccgt gcccgcgccg cggacctgcc agagtacggt gttaacgccc atgtcggcac
     4381 aattgcgaat gatcgtgcgc acgtcgtcgg gatagcgata gtgaaaccgc gccacccaca
     4441 ccgcgcgctc cacctgcggc aacgggcgcg gcgcactgcg agaaaccggc tgccgagcat
     4501 gccgcgcctg ctcgaacatc ggcgacgacg agacctccgg gttccggcag ccaagcaatc
     4561 cgccccaggc caggagcatt cccgacagga tcactcccca ctgtgcagcg cgcatgagtt
     4621 tcatagagcg gaaagtaaac gctcgtcgcg aggaacgtca attccgcacg tcccgccgag
     4681 cacgactgct gacttctgcc tcctcattgc cgtcacggcg gcgttgcgtg cgcggcaggg
     4741 catcgccggt gcgccgccga agtgctaata ttcctcgtag agtcttggcg aggcaatgcc
     4801 ggtgaaattc acaaagatgc acggcctggg aaacgactac gtgctcgtga gtcttttcga
     4861 gcagcacgtg gacgacccgc ccggcttggc gcgcgccatc agtgaccggc atcgcggcgt
     4921 gggcagcgac ggcctgattc ttctggcgcc gcccgattcg cccgaggccg acgcccgcat
     4981 gatcatgttc aactcggatg gctcgcgcgc ccagatgtgc ggcaacggca tccggtgcct
     5041 ggcgaagctg gcctgggagc gcggcttggt gcggcgcaac ccgatgcgca tcgccaccga
     5101 cgcgggggtc ttgagcatcg acctgacgtt ggattcggcc ggcaaggcca ctcaagcgcg
     5161 cgtcgacatg ggcgcgccga ttctgcaacc ggggcgcata ccggtgcgcc tggcggggga
     5221 gcgcgtcgtg gcagcgccgc tggcggtggg cacacgctca ttgctggtta cctgcgtgtc
     5281 gatgggcaac ccacacgcgg tctttttcac cgatcggctg gaagatgtgc cgctggcgga
     5341 ttgggggccg cgcatcgagc ggcatgcgct tttcccagag aaaacgaatg tgcacttcgc
     5401 gcacgtcctg gcgcgcggcc gcgtgcggat gattacctgg gagcgaggcg cagggctcac
     5461 acaagcctgc ggaaccggcg cttgcgccgt aggcgtggcg gccgtgctca acaacctgac
     5521 cgaccggatg cttacggtcc acctgcctgg tggcgacctc caggtagaat gggtggcggc
     5581 cgagccggtg cgcggcgggg ccggctcccc cgcgaacccg gccgctacgg cgggccccgg
     5641 tcaagcccta gccccgcccg agcgcagcaa ccacgtcttc atgactggac cggccgaaga
     5701 agtcttcacg ggcgactggc ccgattaggg cgatggcccg cgatgcccgc ctaaacctcc
     5761 atgatctcct tcgccttttc ctccgtcatt ttgtcgatct tcttgatgaa ctcgtccgtg
     5821 atcttctgca cctgctcttc gccgcgctcc gcgtcgtcct cgctcagcgt cttggctttt
     5881 ttggcggcgt cgaaggactt attcgcatcc cgccggctgg tgcgcaccgc caccttttgc
     5941 tgctccgcaa gttgcttgat gtgattcgta agctggttgc gccgctcgcc cgaaagcggg
     6001 ggaaccggca ggcggatcat cttcccgtca ttctgcggat taatccccag tccgctcttt
     6061 tcgatgcccc gctgtatgtc ctttaatgta cttgggtcga agggcttgat tattatgacg
     6121 ttgccctccg tcacggcgat gttcgccagc tccttcagcg acatcgacga atcgtaggac
     6181 tctacgtcaa tccgtaacgt atccaccagg gccggcgtcg cccgccccgt ccgcaccgat
     6241 cgaaactcct gtttcaggaa ttcgaccgcc ttgttcatct cgtcttgtgc tttcttgacg
     6301 atgtcgttga ctgtcatgaa tcgttctcca gacgcccata acttgggctg ccgcacctaa
     6361 acgcgccggg ggcacaataa ccgccgccgc cgcccgcctc aaccaagcgc cgacgagcct
     6421 ataaacgcgg tggctccggt aatcagcaaa ttgcgggctt acgcaaccac tcaccatcct
     6481 tggccgatac aaccagcaac agtgccacag cggggcaacc cgccgacaga aaaatcgccg
     6541 aacgaactcc gggttgaacc ccaaaaggag gatacgcccg tgcagacaat ttttgtcacc
     6601 ggcgccgaag gctttgccgg caacttgctc acccagcaat tgcggcagag cggataccag
     6661 gtttgggcgg gggttcgaaa ccgcgcccgg aaactcgctc acgagcggca gcaggggcag
     6721 gccatcgtgt gcgacgtcag cgacgccatc aaccttgccc gcgccattgc cagtgccaag
     6781 cccgacgccg tggttcacct ggccgggttt tcccaccccc acgccgccag cgaaatgcca
     6841 ctggaagcct accagtcgat cgtaaccgct tgggcaaacg tcctggatgc ggtccgccgc
     6901 atcactccac gcgcccgtgt gctcctggtc agcgcttgcg acgtgtacgg ccgcgccgga
     6961 gccgacggcc agcctctccg cgaagatacg ccgccccagc ctgtcagcac tttcgggaag
     7021 ctgaagctga cggccgagtc aatcgcccat accttctacg agcgttacca tctgaatctg
     7081 acgatagcac gcaccttcca ttacacgggt gcgggacagc ccgattcgtt cttcttcggt
     7141 tcaatagccc atcgcctggc ttcctgggac ccggccgttc agggcgatac gctggccctg
     7201 cctgacctgc aatgccgccg cgacctgttg cacgtccgcg acgtggtcag cgcttacgaa
     7261 cgcctgctcc aggacggcaa accgaacgag atttacaacg tctgctccgg ccaggcgcac
     7321 acctgtggcg aaatcgtcca gatgatggtc caggcgctgc gtaccccgct tcaaatcacc
     7381 gaacagtgcc cggccgaagg caacgacgag caagtgccgg tcttcctcgg tgataacacg
     7441 aaaatccgaa cggaacttgg ctggagtccc actcagacgt tcgaaggcgc cgtgcaggag
     7501 ctgatccgca gctaccagcg ccgcccaacc gcgcaagcgg tcgctcacga aagcgctgac
     7561 agcgccggtt gaccaaaaca agcgccagtt gaggctgact gggaactaac aagcacagaa
     7621 ctaacaagca caggactaaa aaggactagc aagtacgact gcggaaccag aactaacaac
     7681 taataactaa caactaataa gcattagcaa ctaacgaata gtaaggagtc tggaactaac
     7741 aagtctgaag ctaagggggc agcgggcccg gcgcaaagcc gggcccgcgc gttaggggcg
     7801 cagtgccgtg attgccatat agccaagctg taagcacatg ggtgttaagc ccaagctgca
     7861 aggcatgggt gccatgccca agctgtaagc gcgggcatgc ccttattaac gacctca
//
LOCUS       DAOSYS010000169         7901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7827_length_7901_cov_4.865027, whole genome shotgun sequence.
ACCESSION   DAOSYS010000169 DAOSYS010000000
VERSION     DAOSYS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7827_length_7901_cov_4.865027"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLU99_10835"
     CDS             <1..268
                     /locus_tag="PLU99_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17592.1"
                     /translation="GAAEVVGRDGAASVATHGVPLAQPRRIEHEAASTVRMMVVVRMA
                     VDYPKRRKLASNFWPPGVNKLSGWNCTPKSSGNVPGSIISEWKR"
     gene            complement(298..1371)
                     /gene="sppA"
                     /locus_tag="PLU99_10840"
     CDS             complement(298..1371)
                     /gene="sppA"
                     /locus_tag="PLU99_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOO17593.1"
                     /translation="MITAIRSAQTSHLCAARNVCSTRRRAAFTTASISAAAALLIMLT
                     GCGPTSFMITPVRAERKLEEHVVLREAPLATKKIALIDVEGVLKNDRGGSLLGVQDEN
                     PVALFKEKLDRAARDARVKAIVLRINSPGGTVTASDLMYQEVLRFKQCTGKPVIASML
                     DVAASGGYYLACAADKIYAQPTTLTGSIGVIMLLPGVAGTMEKIGLSMRIFKSGDLKD
                     ASALFREMSPRDREMFNDLVARMYARFLNVVQDGRPELDEEHLRAVADGRVFLGPEAH
                     EQGLVDEVGGIYEAVGAAKAAAGLCDENVLVVEYARPWGYRANVYARSDQPAPQVNMV
                     NIQLPDWLTSPAPQMMYIWAPGW"
     gene            1438..1809
                     /locus_tag="PLU99_10845"
     CDS             1438..1809
                     /locus_tag="PLU99_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOO17594.1"
                     /translation="MIRISDAEFDRAVEEALADIPPDFARYLENVVVEVRARPDRKLM
                     VNEDVPEDALGVYIGVPLDEQGSGALYPLPDRILIFRENLCAMCESREELIDEIRITV
                     LHEVGHHFGLDEDRLDELGYG"
     gene            complement(1888..1972)
                     /locus_tag="PLU99_10850"
     tRNA            complement(1888..1972)
                     /locus_tag="PLU99_10850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1936..1938),aa:Leu,seq:cag)
     gene            2145..3308
                     /locus_tag="PLU99_10855"
     CDS             2145..3308
                     /locus_tag="PLU99_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO17595.1"
                     /translation="MAASFRNGNLFAGTRHENRRVVVTGQVGVDKGPFIDEMARLAES
                     RGDPVRVFHIGQMMYAEAPDVRRGRILDLPLARLNALRRSVFKDVLAQIDTPETVIIN
                     THAAFRWKHGLFPAFDHDQMLDIDADVYVTLVDNADAVHERLMREHDLPHTLKDILVW
                     REEEILATEVLANAIRGHGHFFIYARGNQDAALISLYRLIFRPYMKRVYPSFPMTHVM
                     DMPAVMAEIDAFRATLGEYFITFDPGDMDEKRLLFEAGAATQRGESSFTIKVNGRDLT
                     FSVADVSSVARDIDAQIYARDFKLIDQADMIVTFIPELPDGKPGLSSGVERELQHAWE
                     ATKEVYIVWKPSVEPSPFLTETATRIFRTVPEALGYFQEKGYISNFQPPLFPG"
     gene            3362..4516
                     /locus_tag="PLU99_10860"
     CDS             3362..4516
                     /locus_tag="PLU99_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17596.1"
                     /translation="MHICHVITRLIVGGAQENTLLTCEGLHQRGHRVTLIAGPTTGPE
                     GSLVPRARSGGYEYIEIPDLIRAINPWLDCRARRLLAMEFNRLRPDIVHTHSSKAGIL
                     ARFAARDARVPVVVHTIHGMSFNRTQPALVRRAYAWAELLAARRSHAIVTVANAMIEQ
                     SVAARICRRDKMLTIYSGMEVERFTPRPELRQAARAAWGVSDEQVVVGTIARLFRRKG
                     YEQLLPIMADAAGREPRLHFVWVGDGAQRREYEAELERLGLRKRTTLVGLVPPQRVPE
                     LIAGFDVLAHTSQWEGLPRAVVQALLMQVPAVAFDIDGTPEVVLDNQTGRLIPLNNFA
                     EFATALVELAADANLRQRLGAAGRAHCLPLFDWRTMVDQLEQLYLRLARR"
     gene            4598..6481
                     /locus_tag="PLU99_10865"
     CDS             4598..6481
                     /locus_tag="PLU99_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOO17597.1"
                     /translation="MSSPNDPKTERGRNSSPGAMAGAGPEVIQQFVAELHRRTAHAGM
                     RESADSLTLAEAALRLGRAPSAAGRPLQVALIGPTQVGKSTVFNLLLGREVSPVSPLA
                     GFTIHPFACAIGNAAPGQATAAGDARRQDWTADIFPGWRQTAPADLRRDDLAAYSLVT
                     LDDAIATPLASGAPALGPTVFWDTPDFDSLAAAEYRRGVLETAALADVYVLVLSKEKY
                     SDLSVWNMLKLLAPLARPLIICLNKVTPDSLDAVLRSLRERLAEHGQRWGEVPVFPLE
                     YRPELAAVADPNAAPRSRELIAPLAAELHQTLTRAPKRSLAGVRALVEEHWPAWTAPI
                     RAEHAALAEWNHLVQAGLQQFLERYQQDYLDHPQRYDSFRRAALELLHLLEIPKVSKW
                     IAAARQTVTWPIRQLWGAGRELLRSSPGPQHELGPETTVLIDSLDNMLMWLERELARR
                     SLSDAPGAAVWRAIAARLQTDERRLRDVFETALLAHHEEVKNEVQTAAGQLYEELRKH
                     PARLAALRTGRAAIDAAAVALALKTGGLTPVDAVFAPASFAVSSLLMEGIAGLQMGSV
                     ARDLKKRQLKAVQSKLVQNVLGAELYALAGNLQAKGLLGIPPQRLEEAGRALHAWTAG
                     GKP"
     gene            6478..>7901
                     /locus_tag="PLU99_10870"
     CDS             6478..>7901
                     /locus_tag="PLU99_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOO17598.1"
                     /translation="MIEDFAELVRRSREWFERAAAAGWFAAGDRRRFEAVERATPADL
                     FESEPARPLVVALFGGTGVGKSSLLNRIAGAPIAVVGAERPTSRETTLYVHEAVKLAN
                     LPPELPLNRVQIRRHRSAAQHDVLWIDAPDIDSVEEENRQTALAWLPHVDLLLYVVSP
                     ERYRDDVGWRVLRERGQKHGWMFVMNRWDEGDLRQRDDFRRMLRGAGFEEPLLLCTSC
                     RTPPARLPTPDEFNQVQATIRQLLEAHAVRELARLGVRARVLELQAALAELSRPFGDD
                     AQWAALSSALRREWEATRERLLAGMSWGLQTAAARFAVREGGLLKRVGREVADLRSGP
                     ERKARNAGGQRTALQSPALSDAERPPELSDLAGLTASLWDEWSQAKLTAALDAVEVAT
                     ARAGITSAPVRRQLDPVAERAAQVIHDRMQDEVRVKLLKPHGLAARVARRITGFLTAA
                     LPLAALAAVAYRVVVGYFPRAGQP"
BASE COUNT         1350 a         2570 c         2634 g         1347 t
ORIGIN      
        1 cggcgctgcc gaagtcgtcg ggcgcgatgg ggccgcatcg gtcgccacgc acggcgtgcc
       61 gcttgcgcaa cccaggagga ttgaacacga ggcagccagc acggtcagaa tgatggttgt
      121 cgtccgcatg gctgtggatt acccgaagcg gaggaaattg gcaagcaatt tctggccgcc
      181 aggcgtcaac aaactttcgg gttggaactg cacgccgaag tcgtccggta atgtgccggg
      241 ttcgataatc agtgaatgga aacggtgaca agcatgcggc cctgggcccg acatcactca
      301 ccagcccggc gcccagatat acatcatctg cggcgcggga ctggtcagcc aatccggcaa
      361 ttggatattg accatgttga cttggggcgc gggctggtct gagcgggcgt agacgttagc
      421 ccggtagccc cagggccggg cgtattcgac cacgagcacg ttttcatcac acagtccggc
      481 ggctgccttg gccgccccga cagcttcgta tatgccgccg acttcatcga ccaggccctg
      541 ctcgtgcgcc tcgggcccca ggaacacgcg gccgtcagca accgcgcgca ggtgttcttc
      601 gtcgagttcc ggccggccgt cctgcaccac gttgaggaag cgggcgtaca tgcgtgcgac
      661 gaggtcgttg aacatttcgc ggtcgcgtgg ggacatttcg cgaaagaggg cgctggcgtc
      721 tttaaggtcg ccgcttttga aaatgcgcat gctcaggccg attttctcca tcgtgccggc
      781 cactcccggc agcagcatga tgacgccgat actgccggtc agcgtcgtgg gctgggcgta
      841 gattttgtcc gcggcgcagg ccaggtagta tccgccggaa gccgcgacat cgagcatgct
      901 ggcgatgacc ggcttgccgg tgcactgctt aaaacgcagc acttcctggt acatcaggtc
      961 gctggcggtg acggtgcccc cgggtgagtt aatgcgcagg acgatggcct tgacccgtgc
     1021 gtcgcgcgcg gcgcggtcga gcttttcctt gaacagcgcc accgggtttt cgtcctgcac
     1081 gcccagcagc gacccgccac ggtcgttctt gagtactcct tcgacgtcga tcagcgcgat
     1141 cttcttggtg gccagcgggg cttcgcggag gacgacgtgc tcttcgagct tgcgctccgc
     1201 cctgaccggc gtgatcatga acgaagtcgg cccgcagccg gttagcatga tcagcagggc
     1261 cgctgccgcg gatatcgagg cagtcgtgaa agcggcccgc ctacgggtgc tgcacacgtt
     1321 gcgggcggcg cataaatgcg aagtttgggc cgagcgaatc gcggtgatca tctgttatac
     1381 tcctcctcga cgggctcgcg gccgcggccc atacgctgct tgcattacag ggtgcgcatg
     1441 atacgcattt ctgacgccga gttcgaccgg gcggttgaag aggcgttggc cgatatcccg
     1501 ccggatttcg cgcgctacct ggagaacgtc gtggttgagg tgcgggcgcg cccggaccgc
     1561 aagctgatgg tcaatgagga cgtgccggag gatgcgttgg gcgtgtacat cggggtgccg
     1621 ctcgacgagc aggggtcggg cgcgctgtac ccgctgccgg accgcatcct gatcttccgc
     1681 gagaatttgt gtgccatgtg cgagtcgcgc gaggagctga tcgacgagat tcgcatcacg
     1741 gtcctgcacg aggtggggca tcatttcggc ctggacgagg accggctgga cgaactcggg
     1801 tacggctgag cggtgctgaa cgctagatgt agggccgggc gtaggtcaaa aaaaagggcg
     1861 gtcgcgatta cgaccgccgt tcgttaatgc ggctgactgg agtcgaacca gcacgggctt
     1921 atcgcccaca aggacctgaa ccttgcgcgt ctgccaattc cgccacagcc gcggaagacc
     1981 gggccggcag caacttgcgt ttgccagtgc ccgacgaaag aatcatgata aaggtttgtc
     2041 ggccaacgtc aaggaatagc cgcgggccgg gtttgccact gggccgttgg gcgtgccggt
     2101 gcggtggccg gcaaaggcgg tttggcttga aagtgcggga gtgaatggcg gccagctttc
     2161 ggaacggaaa cctatttgct ggaacgagac atgaaaaccg ccgggtcgtc gtgaccgggc
     2221 aggtgggcgt ggacaaaggg ccgtttatcg acgagatggc ccgactggcc gagtcgcggg
     2281 gcgacccggt gcgcgttttt cacatcgggc agatgatgta cgccgaggcg cccgacgtgc
     2341 gccgcggccg gattctcgat ctgccgctgg cccggctgaa cgccctgcgg cgctcggtgt
     2401 tcaaggacgt tttggcgcag atcgacacgc cggagacggt catcataaac acgcatgccg
     2461 cgttccgctg gaagcacggg ctgttccccg ccttcgacca cgaccagatg ctcgacatcg
     2521 acgcggacgt gtacgtcacg ctggtcgaca acgccgacgc ggtgcacgag cggctgatgc
     2581 gcgagcacga cctgccgcac acgctgaagg acatcctggt ctggcgcgaa gaggaaatcc
     2641 tggcgactga ggtgctggcc aacgcgattc gcgggcacgg gcatttcttc atctacgccc
     2701 gcggcaacca ggacgccgcc ctgatctcgc tctaccgcct gatcttccgt ccgtatatga
     2761 aacgcgttta cccgtccttc cccatgacgc acgtgatgga catgcccgca gtcatggccg
     2821 aaatcgacgc cttccgggcc acgctgggcg agtacttcat cacgttcgat cccggcgaca
     2881 tggacgaaaa gcggctgctg ttcgaggcgg gcgcggccac gcaacgcggc gaatcgagct
     2941 tcacaataaa ggtgaacggg cgcgacctga cgttcagcgt ggccgacgtg agcagcgtgg
     3001 cccgcgacat cgacgcccag atctacgccc gcgacttcaa gctgatcgat caggccgaca
     3061 tgatcgtgac gttcatcccg gagctgccgg acggcaaacc ggggctctct tcgggggtgg
     3121 agcgcgagtt gcagcatgcc tgggaggcga ccaaagaggt ttacatcgtc tggaagccca
     3181 gtgtcgagcc ctcgcccttt ttgactgaga cggccacgcg catcttccgc acggttccag
     3241 aggcgctggg ctactttcaa gagaaaggct acatcagcaa cttccagccg ccgttgtttc
     3301 cggggtagcg ggcaccggcg ttcgctaccg gtcagggcca ccgcccggca taatgccgcc
     3361 catgcacatt tgccacgtta ttacccggct gatcgtcggc ggcgcccagg agaacacgct
     3421 cctcacctgt gagggtctgc accagcgcgg ccaccgcgtc acacttatcg ctggcccgac
     3481 caccgggccc gagggctcgc tggtaccgcg cgcccgtagt gggggctacg aatacatcga
     3541 aattcccgac ctgatccggg ccatcaaccc gtggctcgac tgccgtgccc gccgcctgct
     3601 cgcgatggag tttaaccgcc tgcgccccga tatcgtgcac acccacagca gcaaggccgg
     3661 catcctggcc cgctttgcgg ctcgcgacgc tcgcgtcccc gtggtcgttc acactatcca
     3721 cggcatgagt ttcaaccgca cgcagccggc cctggtcagg cgagcgtacg cctgggccga
     3781 attgctcgcc gcacgccgca gccacgccat cgtgaccgtg gccaacgcca tgatcgagca
     3841 aagcgtggcc gcacgtatct gccggcggga caagatgtta acgatctact ccggcatgga
     3901 agtcgagcgc ttcacgcccc ggccggagct gcgccaggca gctcgggcgg cctggggcgt
     3961 cagtgacgag caggtggtag tcggcacaat cgcgcgcctc ttccgccgaa agggctacga
     4021 gcaactgctg ccgattatgg cggatgcggc cggccgcgaa ccgcgcctgc atttcgtctg
     4081 ggtcggcgac ggcgcccagc gccgcgaata tgaagcggaa ttggaacggc tcggcctgcg
     4141 aaagcggacg acgcttgtcg ggctggtccc gccgcaacgc gtgccggaat tgatcgccgg
     4201 ctttgacgtt ctcgcgcaca cgtcgcaatg ggagggtttg ccgcgggccg tcgttcaggc
     4261 gctgctgatg caggtgccgg ccgtcgcttt tgatattgac ggcacgccgg aagtcgtcct
     4321 ggacaatcaa accgggcggc tgatcccgct taacaatttt gccgaatttg cgactgcttt
     4381 ggtcgaactg gccgccgacg ccaacctgcg ccagcgcctc ggcgcggccg ggcgcgccca
     4441 ctgtttgccg ctgttcgact ggcgcacgat ggtcgaccaa ttggagcaac tgtacctgcg
     4501 cctggcaagg cggtagcggc cctcatccct tgaccgaatc gcgcatgcca ctaagcggcc
     4561 ctgtcgaatt ttctccgcct cgaacagaat aaggattgtg agcagcccta acgacccaaa
     4621 aaccgaacgc ggccggaact cgtcgcccgg cgcaatggcc ggcgccgggc ctgaggtcat
     4681 ccagcagttc gtcgctgagc tgcaccgccg caccgcccac gccggcatgc gcgaatccgc
     4741 cgattcgctc acactggccg aggccgcctt gcgcctcggc cgggcgccca gcgcggccgg
     4801 ccggccgctg caagtcgcgc tgatcggccc gacgcaggtc ggcaagagca ccgttttcaa
     4861 cctgctgctg ggccgcgagg tcagcccggt cagcccgctg gccggcttca ccatccaccc
     4921 cttcgcgtgc gccatcggga acgccgcgcc cggacaggcg acggcagccg gcgacgcccg
     4981 ccgccaagat tggactgcgg acatcttccc cggctggcgc caaaccgcgc cggctgactt
     5041 gcgccgcgac gatctcgccg cctactcgct ggtgacgctc gacgacgcca ttgccacccc
     5101 tcttgcctct ggagcgcccg cgcttgggcc caccgtgttc tgggacacgc ccgatttcga
     5161 ttcgctggcg gccgccgagt atcgccgcgg agtgctggaa acggcggccc tggctgatgt
     5221 ctatgtcctg gtgctcagca aggaaaagta ctccgatctc tcggtctgga acatgctcaa
     5281 gctgctcgcg ccactcgcca ggccgctgat catctgcttg aacaaggtga cgcccgattc
     5341 gctcgacgcc gtgctgcgat cattgcgcga gcgcctggcg gagcatggcc agcgctgggg
     5401 cgaggtgccg gtattcccac tggagtaccg gccggagctg gcggccgtcg cggatccgaa
     5461 cgccgccccc cgcagccgag aattgatagc acctctagcg gccgaactgc accagacgct
     5521 cacccgcgcg cccaagcgtt cgctcgccgg cgtgcgggcg ctggtcgaag aacattggcc
     5581 ggcgtggacc gcgcccatcc gggctgagca tgccgccctg gcggagtgga accacctggt
     5641 gcaagccggt ttgcagcagt tcctggaacg ctatcagcag gactacttgg atcacccgca
     5701 gcgctacgac agcttccgcc gcgcggcgct ggaactgttg cacctgctcg aaatacccaa
     5761 ggtcagcaag tggatcgcgg cggcccggca gaccgtgacc tggcccattc gccagctatg
     5821 gggcgcgggc cgcgaattgc tgcgctccag ccccggaccg cagcacgagt tggggcccga
     5881 aacaaccgtg ctgatcgatt cactggacaa catgctcatg tggctggagc gcgagctggc
     5941 acgccgcagt ctgagcgacg cacccggagc cgcggtgtgg cgggccatcg ccgcacgcct
     6001 gcagaccgat gagcgccgcc tgcgagatgt attcgaaacg gccctcctgg cccaccatga
     6061 agaagtcaag aacgaagtcc agaccgctgc cggccagttg tacgaggagc tccgcaagca
     6121 tccggcccgc ctggccgccc tgcgaacggg gcgggccgca attgacgcgg ccgccgtggc
     6181 cctggcgctg aagaccggcg ggcttacgcc ggtggacgcc gttttcgccc cggccagctt
     6241 tgccgtcagc agtctgctga tggagggcat cgccggctta cagatggggt cagtggcccg
     6301 cgatcttaag aagcgtcagc tcaaggccgt gcagtcgaag ctcgttcaga acgtgctcgg
     6361 cgccgaactg tacgccctgg ccggaaatct ccaggccaag gggctgctgg gcattccgcc
     6421 gcaacggctg gaggaggccg gccgcgcttt gcacgcgtgg acggcgggag gaaagccgtg
     6481 atcgaggatt tcgcagaact cgtgcgccgc tcgcgtgagt ggtttgaacg ggctgcggcc
     6541 gcgggctggt tcgccgcggg cgaccgccgg cgcttcgagg cggtcgagcg cgccacgccg
     6601 gccgacttgt ttgaaagtga gccggcccgg ccgctggtgg tggccttgtt tggcggaacg
     6661 ggcgtgggca agagcagtct gctgaatcgc atcgcgggtg cccccatcgc ggtcgtcggc
     6721 gccgagcggc cgacttcgcg cgaaacaacg ctgtatgtac acgaggcggt caagctggcc
     6781 aatctgccgc cggagttgcc gctcaaccgc gtgcagatcc gccgccacag gtccgccgcc
     6841 cagcacgacg tgctctggat cgacgccccc gacatcgaca gcgttgagga ggaaaaccgg
     6901 caaaccgcgc tggcctggct gccacatgtg gacctgctgc tgtacgtcgt cagtcccgag
     6961 cgctatcgcg acgacgtcgg ctggcgcgtg ctgcgtgagc gcgggcagaa gcacggctgg
     7021 atgtttgtaa tgaaccgctg ggacgagggt gacctgcggc aacgcgatga cttccgccgc
     7081 atgctgcgcg gcgccggctt cgaggagccg ctgctgttgt gcacttcgtg ccgcacgccg
     7141 cccgcgcgcc tgccgacgcc cgacgaattc aaccaggtcc aagccaccat tcgccagttg
     7201 ctggaggcac acgcggtgcg cgagttggcc aggctgggcg tacgggcgcg agtgctcgaa
     7261 ctgcaggctg cgctggccga gctgtcgcgg ccattcggcg acgatgcgca gtgggcggcc
     7321 ttgagcagcg cgctgcgccg cgaatgggag gcaacacgcg agagactcct cgcaggcatg
     7381 agctggggcc tgcagacggc cgcggcgcgc tttgcggtgc gcgaaggcgg cttgctgaaa
     7441 cgcgtcgggc gcgaggtcgc ggatttgcga agcggccccg agcgaaaagc caggaatgca
     7501 ggcgggcaac gcactgcatt gcagagccct gctttgtctg atgccgaacg tcccccggag
     7561 ctatccgatc tggcgggcct gacggcctca ctgtgggatg agtggagcca ggcgaaactg
     7621 actgccgcac tggacgcagt cgaagtcgcg actgcgcggg ccggcattac cagtgccccg
     7681 gttcgccgcc aacttgaccc ggtcgcagag cgcgccgcac aagtcataca tgatcgaatg
     7741 caggacgaag ttcgcgtgaa actgttgaag ccgcacggac tggccgcacg cgtcgcccgc
     7801 cgcattacgg gttttctgac ggccgctttg ccgctcgccg ctttggctgc cgtggcgtat
     7861 cgcgtcgtgg tcggctattt cccgcgcgcc ggccagccgc a
//
LOCUS       DAOSYS010000170         7861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7881_length_7861_cov_6.655393, whole genome shotgun sequence.
ACCESSION   DAOSYS010000170 DAOSYS010000000
VERSION     DAOSYS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7881_length_7861_cov_6.655393"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="PLU99_10875"
     CDS             complement(<1..1390)
                     /locus_tag="PLU99_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17599.1"
                     /translation="MSPCALRAPYRPIWPISAAVAGVLFLMTSGLCPAAEPGADATQP
                     RADATAVPLLFPDDQPQRLAARAVEVVRRFDFDERVFGNYEDTPLHWERLEGPGLPRY
                     SNARLDDECGRLAPPSFRFDVQGGSVAYEYAGADLLLTPGAEHYLVGYARAVDLREAR
                     AFVQVSLLDAAGRAIPGSDRISDLLASTLCTGNSDDGAGPDSEPWQRIEVRLPTDFPD
                     AAAVRLTLWVLQAYAWREAPEGDPIVRQDVDARVWFDDLTLYRLPHVRLTLSTGSTLI
                     RREIPASFLIQVRNPAPAALEFDLSLRDVFGHVVHEQGGTIPAGASSPLENSVPLLEP
                     GTYTAHLSLRDESESFGNSSLRFAVLEELPQGNSGAADFGIDLGELQPGDADALVQLV
                     GELGCQAAKLGLTLPDDGISSPNLEAVRDLVRRLLSAGIQPTGVILPPPPAGSIRTAL
                     MAPDRSDQLAPLL"
     gene            complement(1474..2244)
                     /locus_tag="PLU99_10880"
     CDS             complement(1474..2244)
                     /locus_tag="PLU99_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO17600.1"
                     /translation="MGLRLTILGSGTSHGIPMIACDCAVCTSADPHDRRTRASALFSF
                     EGRHLLVDTSPELRLQCLACGVRQVDAILYTHAHADHVAGLDDVRRFNWLQGRALDVY
                     GAPATLKRIHTMFRYAFENEPEYPSAKPDLNLAPISGPFELFGRQVVPIPYQHGQQTV
                     LGFRIGDIAYCPDCSFIPDESRKLLSGLEVLVLDGLRRRPHPTHFNLDQAVAEAGRIG
                     ARRTYLTHIAHELPHAATNAELPPEIELAYDGLVCTAA"
     gene            complement(2257..4128)
                     /locus_tag="PLU99_10885"
     CDS             complement(2257..4128)
                     /locus_tag="PLU99_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17601.1"
                     /translation="MARGSVNIFEQHVEKITLGLAAVFLLLMVYLYMVRSPNRVSYGN
                     QKLAPAELTEAIQRDTEALKKAMSNAPAVDTDVPDYSAQLLEQHSAGLFKRQAGQEPL
                     VPDRLPWAADFGLPIPNLAEEQEEGQSDIALVPPLAPTAPVSRAGRNLVIRERSSLTQ
                     TSEDDEGGPASEAVELPWVTVAAYFDRAAQQDAMTKAGYATYLSKIYIAGVDVQRQEL
                     LADGNYSEWQDVTPSMAMPQVGTPAPEIDANSGVLINRDALNNVFATIKANQASICQP
                     PFYRIEAGEEWRMPPLDGYPDADGPKTAAKGAKQPSKEDGRKGPIKVDPNPVDDKTAR
                     DKATQALKRAEDAYRSGQDDAALQAAEEVLGNKAATARARNRARAIQRSVRLMEKQKG
                     ASAKPAIPVQTRKEGSQEANLVAHPDDPNKVAVWFHDDTVESGKSYRYRLRVNLWNRY
                     LGKFAALKDREQAKLPVLLGDWSLPGEPVTVPPAAYFFVTSAPPGKDTVGVTVYKWHK
                     GERVSKIFYVGVGDLIGQVQEGETGILDRETLQPTRESVDYSTGALVLDLRLNQPVVQ
                     RNISDKEKGEFSLRDTESAVLVYLDPADGQVKQRVERFDRYDPTLRALRDLEEGTEK"
     gene            complement(4162..5499)
                     /locus_tag="PLU99_10890"
     CDS             complement(4162..5499)
                     /locus_tag="PLU99_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17602.1"
                     /translation="MAFIKAHLILLITGVAALLFIVLAVLGMTSDSVVKDMEKRASLG
                     SQLTSLKSTPRNEESIAAEKSRVARLESEYAEVLAAAAEINARTPLKEGVFPTPERAA
                     LAYDFCDAYRKAMADLPRGKLAGGNLPSEAEIAEAKDQIAELEERWKIEHGDHGPTLP
                     RVGTGTTPPAGQTGAPPTAAGSGAPGSGDASKDALARARITKARNIRCYVGTDPAQSG
                     YSFSLSPIWHIAGEQPQPADMWYAQVALWIEQDVVAAVAELNDQVADQLPPEEVYVEN
                     MPVKRLQTVAVHGYYSEKGLLPFPLDVRTSTGLPQAFQGESFTGRRCNKDFDVVRFTV
                     VAVVDQRALTQLIDAITRQNSYVLISSKYEALGPGAPDYSNGYFYGTAPVARLTLDFE
                     GYMARNVYAKWFPPAVRAQLGIADETGGKGKPGGKSKPPRRRPADKTESDDLP"
     gene            complement(5547..7523)
                     /gene="pilM"
                     /locus_tag="PLU99_10895"
     CDS             complement(5547..7523)
                     /gene="pilM"
                     /locus_tag="PLU99_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOO17603.1"
                     /translation="MAASNAVWGIDIGQCALKALKLRNAGDGKVEAVAFDLIEHAKIL
                     SQPDAEPDELIRAALEKFVTRNDWQGDRFVLGVPGQQTFARFCKMPPVDPKKIPDLVK
                     FEAGQQIPFDMDDVVWDYQTFATPESPDLEVGIFAMRKDLIRKHVGFLSAVGISPAIV
                     QTVPCALYNFSCFERPSDDPKAATVIISVGAQNTELVIVEPNSAWTRNIPLGGNNFTE
                     ALVRAFKLSFAKAESLKRTAASSKYARQIFQAMRPVFAELVAEIQRSIGFFSSTHRDV
                     ELRSALALGNAFRLPGLQKYLENNLTISGGVIKLERFNQMIPTATVNAPQFSENVLTF
                     APAYGLALQGLGLAKINASMLPTELARLAIWNRKRPLFIAAAACLGVAALLPWGRAML
                     DRQALASADNVIQKANEAIQQANKYKSEYQKVQSNTKGQEDAIKDLLAMRDNVAVVPQ
                     ILTLPFMALPEPQPALKAINSMADMKKLWESGAAPSRKERGQFLIDTVDLALVDEVDK
                     VNLGAAGVREGLSAGVARTGKAIDRRNAGFVVAVSGRLLYGDTRAQVSDFLENEYFSR
                     LRKLASEAGRGFYIPDDQGAIATPRFRRAAPSQLSGSGAAGGADASQKDPVTGEDVST
                     DWRVEFEFKLKLGEKPVPGEEKTEPAKKSGKKKP"
BASE COUNT         1370 a         2494 c         2435 g         1562 t
ORIGIN      
        1 gcagcagcgg cgctagctga tcggaccgat cgggcgccat cagcgccgtt ctgatcgagc
       61 cggcgggcgg cggcggcaga ataacgccag tcggttggat gcccgcgctc agcagtctgc
      121 gcacgagatc gcgcaccgct tcgaggttgg gactggatat gccgtcatca ggcaacgtga
      181 gacccagctt ggccgcctgg caccccagtt cgccaacaag ctgcacgagc gcgtcggcgt
      241 cgcccggctg aagctcgccc agatcgatgc caaagtctgc agcgccagaa tttccctgcg
      301 gcaactcctc gagcaccgcg aatcgcaaac tgctgttgcc gaacgactca gactcatcgc
      361 gcagagagag gtgagcggta tacgtgccgg gctccagcag cgggaccgaa ttttccagcg
      421 gcgagctcgc gcccgcgggt atggtgcccc cctgctcgtg cacgacatgc ccgaacacat
      481 ccctcagcga caggtcgaac tcaagcgccg ccggggcagg attgcggact tggatgagga
      541 atgacgccgg gatctcgcgc cggataagcg tgctgccggt agaaagtgtc agccgaacgt
      601 gcggcagccg gtagagcgtc aagtcgtcaa accacacgcg agcgtcaacg tcttgccgca
      661 cgatcggatc gccttcgggt gcctcgcgcc atgcgtacgc ctgcaagacc caaagtgtga
      721 gtcgcacggc ggcggcgtcc ggaaaatccg tcggcaggcg gacctcgatt cgctgccaag
      781 gctcggaatc gggaccggcg ccgtcgtcgc tgttgccggt gcacagtgtc gatgccagca
      841 agtcgctgat tcggtcactg ccgggtatcg cgcggccggc ggcatccagg agcgatactt
      901 gcacgaaggc ccgcgcttct cgcaagtcga cagcgcgagc gtagccgacg agatagtgct
      961 cggcgccagg cgttagcagc aggtccgccc cggcgtattc gtaggcgacg ctgccaccct
     1021 gaacgtcgaa gcggaaggac ggcggcgcca ggcggccgca ctcatcgtcc agcctcgcat
     1081 tggaataacg cggcagccca gggccttcga gccgctccca atgcaacggc gtgtcctcgt
     1141 agttcccgaa aacgcgctcg tcgaaatcaa agcggcgaac cacctcgacg gctcgggccg
     1201 ccaagcgttg tggctgatca tcggggaaaa gcagcggaac cgctgtggcg tcggctcttg
     1261 gctgggtggc gtcggctccc ggctcggccg cagggcatag gccggaggtc attagaaaca
     1321 gcacgccggc gaccgcggcg gagatcggcc agataggacg atagggcgca cgaagcgcgc
     1381 acggactcat tattgctcat atcggaccgt gtgaacccag cctgaagcag ccctttcggc
     1441 tgcggccgga agtcgcccgc gatggccgca aactcaggcc gcagtgcaga ccagaccgtc
     1501 gtacgcgagt tcaatctccg gcggcaattc ggcattcgtc gccgcgtgcg gcagttcgtg
     1561 ggcaatgtgc gtcaggtacg tccgccgcgc cccgatgcgc ccggcttcag ccacggcctg
     1621 gtccaaattg aaatgggtcg gatgcgggcg gcgccgcagt ccgtccagga caagcacctc
     1681 caggccgctc aggagcttgc gggactcgtc tggtatgaag ctgcagtccg gacagtacgc
     1741 gatgtctccg atgcggaaac cgagcaccgt ctgctggccg tgttgatacg gaatgggaac
     1801 cacctgccgg ccaaacagct cgaacgggcc gctgatcggc gccaggttga ggtctggctt
     1861 ggcgctgggg tattcgggct cgttttcgaa ggcataacga aacattgtgt gaatgcgctt
     1921 caaggtcgcc ggagcaccat agacgtccag ggcccgcccc tgaagccagt tgaagcggcg
     1981 cacgtcgtcg agcccggcga cgtggtcggc atgggcgtgc gtgtacagga tcgcgtccac
     2041 ctgccgcacg ccgcaagcca ggcattgcaa ccgcagttcc gggctggtgt ccaccagcag
     2101 gtgccgccct tcaaagctga acagagcgct ggcgcgggtg cggcggtcat gcgggtcggc
     2161 ggacgtacag acggcgcaat cgcacgcaat catcggaatg ccgtgcgaag tgccggagcc
     2221 gagaatcgtc agccgcaggc ccaagcgcca cgctctttac ttttcggtcc cttcctcgag
     2281 gtcgcgcagc gcgcggaggg tgggatcata acggtcgaaa cgctccacgc gttgcttgac
     2341 ctgaccatcg gccggatcca ggtacaccaa cacggccgac tcggtgtcgc gcagcgaaaa
     2401 ctcgcccttt tccttgtcgc tgatgttgcg ttgcacgacg ggttggttaa gccgcaagtc
     2461 gagcaccagc gcacccgtgg aatagtcgac ggactcgcgc gtcggctgca gcgtctcgcg
     2521 atcaaggatg ccggtctcgc cttcctgcac ctgaccgatc agatcaccaa ctccaacata
     2581 gaagattttg gacacgcgtt cgcccttgtg ccacttgtag accgtcacgc cgaccgtatc
     2641 ctttccgggc ggggcggacg taacaaagaa gtaagcggcc gggggcacgg tcacgggttc
     2701 gccgggcagc gaccagtcac ccagaagaac gggcagcttg gcctgctcgc ggtctttgag
     2761 ggctgcgaat ttgcccagat agcgattcca cagattgacc cgcaagcgat agcgatacga
     2821 tttacctgac tccactgtgt catcgtggaa ccacacggcc accttgttgg gatcatcggg
     2881 gtgcgctacc aagttggctt cctgactgcc ctcttttcgc gtctgcactg gaatggcagg
     2941 tttcgcgctg gcgcctttct gcttttccat cagccggacg ctgcgctgga tggcgcgggc
     3001 tctgttgcgc gctcgcgcag tcgcggcctt gttgcccagg acttcctcgg ccgcctgcaa
     3061 ggcggcgtcg tcctggccgc tgcggtaggc gtcctcggcc cgcttgagtg cctgggttgc
     3121 cttgtcgcgg gccgtcttgt cgtcaacagg attcgggtcg acctttatgg ggccttttct
     3181 gccgtcctct ttcgacggct gtttggcgcc cttggcggcc gtcttggggc cgtcggcatc
     3241 gggatagccg tccaaggggg gcattcgcca ctcttcgccg gcttcaattc tgtagaacgg
     3301 cggctgacag atgctggcct ggttggcctt gatggtcgcg aaaacgttgt tcagcgcgtc
     3361 gcggtttatg agcactccgc tatttgcatc gatttccgga gcaggggtgc cgacctgcgg
     3421 cattgccata ctgggcgtaa cgtcttgcca ctcggaatag ttcccatccg ccagcagttc
     3481 ctggcgctgc acgtccacgc cggcaatgta aatcttggac agatacgtcg cgtagccggc
     3541 cttggtcatg gcatcttgct gcgccgcgcg gtcgaaatag gccgctacgg tgacccaagg
     3601 caattcaacg gcctcgcttg ccggcccgcc ttcgtcatcc tcggaggtct gcgtcagtga
     3661 tgaccgctcg cggatgacga ggtttcggcc ggcgcgcgat acgggagccg tcggcgccag
     3721 cggcggcacg agagcaatat ccgactgtcc ctcctcctgc tcctctgcca ggttcggaat
     3781 gggcaggccg aaatcggccg cccacggcag ccggtccggc accaagggct cttgaccggc
     3841 ttggcgcttg aacaaaccgg cggaatgctg ctccaaaagc tgggcgctgt aatcggggac
     3901 gtcggtatcg accgccgggg cattgctcat ggccttcttc agcgcctcgg tgtctctttg
     3961 gatggcctcc gtcaattcgg cgggcgcgag cttttggttg ccatagctga cgcggttcgg
     4021 cgagcgcacc atgtacaagt agaccatgag cagcaagaag actgccgcca accccagagt
     4081 gatcttctct acgtgttgct caaagatatt aaccgagcct ctagccattg ttcggtcctc
     4141 tgtcaggggc ggtgctgcgc gtcacggcaa atcgtccgat tcggtcttgt cggccggtct
     4201 tcttctgggc ggtttgctct tgccgcccgg cttgcccttg ccgccggtct catctgctat
     4261 tcccaactgg gcgcgcaccg caggcggaaa ccacttggcg tacacattgc gtgccatgta
     4321 accctcgaaa tccagcgtga ggcgcgcaac cggcgcagtg ccgtagaaat acccattgct
     4381 gtagtccggg gctccggggc ccagcgcctc gtatttgctg gagatgagca catagctgtt
     4441 ctgtctggta atcgcgtcta ttagctgggt cagggcgcgt tggtctacga ccgccaccac
     4501 cgtgaagcgc accacgtcga aatctttgtt gcaacgccgc ccggtgaatg attcgccctg
     4561 aaaagcctgc ggaaggccgg tacttgttcg cacgtcaagc gggaagggca gcaagccctt
     4621 ctcactgtag tagccatgta cggcaacggt ctgcagacgc ttgaccggca tgttctcgac
     4681 gtagacttcc tcgggcggaa gctggtcggc cacctggtca ttcaactcgg ccaccgccgc
     4741 gaccacgtcc tgctcgatcc aaagcgcgac ttgcgcatac cacatatcgg ccggctgcgg
     4801 ctgctcaccg gcaatatgcc agatggggct gaggctgaag ctatagccgg actgggccgg
     4861 gtcggtgcct acgtaacagc gtatgttgcg cgctttggta attcgggccc gggccagggc
     4921 gtctttgctg gcgtcgccgc taccgggggc gccgctaccg gccgcggtgg gcggcgcgcc
     4981 cgtttgcccg gccggcgggg tcgtaccggt tccgacgcgc ggcagggtcg ggccatgatc
     5041 gccgtgctcg attttccagc gctcctccag ttcggcgatt tggtctttgg cctccgcgat
     5101 ttcagcctcg ctcgggaggt tgccgccggc cagtttcccg cgcggcaggt cggccatggc
     5161 cttgcggtaa gcgtcgcaaa aatcgtacgc gagcgcggcg cgctcggggg tcgggaacac
     5221 gccttccttc agcggcgtac gcgcattgat ctccgcggcc gcggcgagca cttccgcgta
     5281 ttcgctctcc aagcgcgcca cgcggctttt ctcggcggca atgctctcct cgttgcgcgg
     5341 cgtggacttc agggaggtca attgcgatcc cagggaggcc cgcttttcca tgtccttgac
     5401 gacgctgtct gaggtcatcc ccagcacggc cagaacaatg aacagcagtg ccgctacgcc
     5461 ggtgatcagt agtatgaggt gtgctttgat gaacgccatc gctgtctcct gtgcttacgc
     5521 ggacggacaa cgcgttgccg cgcgcctcag ggctttttct tgcctgattt cttggctggc
     5581 tcggtttttt cctcgcctgg aacgggtttt tcaccaagtt tgagcttgaa ctcgaactcc
     5641 acgcgccagt cagtgctcac gtcctctccg gtaaccggat ccttctgcga cgcgtccgcg
     5701 ccacccgccg cgccgctgcc cgacaattga ctcggcgcgg ctctccggaa gcgcggcgtg
     5761 gcgatcgctc cctggtcgtc tgggatgtag aaaccccggc ctgcttcact ggcgagtttc
     5821 cgcaacctgg agaagtactc attctccagg aagtctgata cttgcgctcg cgtatccccg
     5881 tagagtagac gtcccgaaac cgcaacgacg aaaccggcgt tcctccggtc gattgccttg
     5941 ccggtccgag ccacacctgc gctgagcccc tcgcgcacgc cagcggcgcc cagattaacc
     6001 ttgtcaacct cgtccaccag agccaagtct acagtgtcga tcagaaactg cccgcgttcc
     6061 ttgcgactgg gggctgcccc actttcccac agctttttca tgtcggccat cgaatttatc
     6121 gccttcaggg caggttgcgg ttcgggcagc gccatgaacg gcagagtcag aatctgcggc
     6181 accactgcga cgttgtcacg catcgccagg aggtccttga tcgcgtcttc ctgacccttc
     6241 gtattagact ggactttctg gtactcgctc ttgtatttat tcgcttgttg aatggcctca
     6301 ttggccttct gaattacatt gtcagcagag gccaatgctt ggcggtcgag catggcacgc
     6361 ccccagggca acagtgctgc cacgcccaag caggccgccg cggctataaa caacggccgc
     6421 ttgcgattcc atattgccag gcgggccaac tcggtgggca gcatgctggc attaatcttg
     6481 gccagcccga gcccctgcag cgccaggccg tacgccggtg caaacgtcag gacgttctcg
     6541 gaaaactgcg gcgcgtttac cgtggcggtc gggatcatct ggttgaagcg ctccagcttg
     6601 atgaccccgc ccgaaatggt caggttgttc tccaggtact tctgaagccc cggcagccga
     6661 aaagcgttgc ccaacgccaa cgcgctgcgc agctcgacgt cgcggtgcgt cgagctgaag
     6721 aagccgatgg agcgctgaat ctctgccacc aactcggcaa agacgggccg catggcttgg
     6781 aatatctgcc gcgcgtactt gctgctggcg gccgtacgct tcaggctttc cgccttggcg
     6841 aacgaaagct tgaaagcccg caccaaggct tccgtgaagt tgtttccgcc gagcggaatg
     6901 ttgcgcgtcc aagcgctgtt cggctccaca atgaccagct cagtattctg agccccgacg
     6961 ctaatgatga cggtggccgc cttgggatcg tcactgggcc gttcgaaaca cgagaagttg
     7021 tacagcgcac aaggtacggt ctgcacgatg gccggcgaaa tcccgacggc gctcaggaag
     7081 ccgacgtgct tgcggatcag gtccttgcgc atggcgaaga tgccgacttc caagtcggga
     7141 gattccggag tggcaaacgt ctgataatcc cagacgacat catccatatc gaaggggatt
     7201 tgctggccgg cttcgaactt gaccaggtcg ggaatcttct tcgggtcgac cggcggcatc
     7261 ttgcagaaac gtgcgaacgt ctgttgcccc ggcacgccca gcacgaaacg gtcgccttgc
     7321 caatcattgc gcgtgacgaa cttttccagc gccgcccgaa tcagctcgtc cggctcggcg
     7381 tcgggctgcg ataggatctt ggcgtgttcg atcaggtcga aggcgaccgc ctccaccttt
     7441 ccgtcgcccg catttcgcag tttaagggct ttcagcgcac attgcccaat gtctataccc
     7501 cataccgcgt tcgaagcagc cattgttcac gctccttccg cgcacggacc tcacgcgccg
     7561 ttattggggc gcgcgcacgt cctgcgaagt tccgagaccg ggagcatgta cgcaaggccc
     7621 ggtcgatcat tcacatatag aggttgcaac cacaactagc cgttgtagtc tgagccgtgg
     7681 tatcaccacg acagctcgct tggggcggga atgatgcgta tgaacgaatg aagccgtctt
     7741 cgtgccctac ccggcgggac tatctggctg cgggtctttt gaagattcag tttgctgggt
     7801 tctgcgcctc ctgcgttcgg ctactccggt ctcgccttcc tccttataaa cttaccggta
     7861 c
//
LOCUS       DAOSYS010000171         7859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7888_length_7859_cov_5.588672, whole genome shotgun sequence.
ACCESSION   DAOSYS010000171 DAOSYS010000000
VERSION     DAOSYS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_7859_cov_5.588672"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLU99_10900"
     CDS             complement(<1..253)
                     /locus_tag="PLU99_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17604.1"
                     /translation="MSKGWRTGLLVGAGAALVVFGLAGGLHAQGRAQTGCCVAVVDIG
                     RLFNEYERMKSAQDELKVLQDRLQRENEERKQKSDMLEAT"
     gene            complement(472..2067)
                     /locus_tag="PLU99_10905"
     CDS             complement(472..2067)
                     /locus_tag="PLU99_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO17605.1"
                     /translation="MEQANRAFCKHCRTLVPCEHVKHDGHVYLVKHCPTCGDNESMVA
                     SDEARWQRKRDFWGYEDPKKNCNLRCTECRKPHNPTIVLIDITNRCNMGCPICIANIR
                     GMGFEYNPPLAYFEKIFQALCHLDPKPLVQLIGGEPTVRHDLLDMVKLGRTYGLKMSV
                     ITNGLRLADEEYCKELCEARVRLRFGLDGRSPEIYGCMRGGPGACEKKLKGLANLAKY
                     SRIKHTILCCAARGINDKWIADLVECCHENNRVIEQLALLPLAETWEEGRFKTQVQTT
                     REDAEAMVEESIPGGQVEFVPAGFIQELRPLYTFLTGRSTSESLMFAGVHPDCETMTL
                     LASDGQRYVSINHFLKMPFSRVAQEVFKRAKQTQPIVARLNPASRIDRWRAKWLAARM
                     FLPLVVRGLAIRKAFRGNPVVVLLKFLGGLLRGRRAGDLARQYFNLSRILRVAVIPFE
                     EYHEIDAARIENCRSCYAYEDVSDGQIKMIPACSWTSTYRDEMIRKVAAKYGTVPVTK
                     PGTIAVQPPEKAACASKCELTPA"
     gene            2552..3388
                     /locus_tag="PLU99_10910"
     CDS             2552..3388
                     /locus_tag="PLU99_10910"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOO17606.1"
                     /translation="MKRVAILASPDKPAAVQTLGRMQQWLRGRAQVVFAGLTYDGRQA
                     LPHRPELLFSLGGDGTLISAAHSLGEQQLPIVGVNLGKLGYLAEFTMEELEREGDFLF
                     DGVLPLTRRIMMEVVLERREQPVFRTLAINDCVVLAGPPFHMIEVAVTVDGDEVTHVR
                     GDGLIVATPSGSTAHNLAAGGPILEPTGAAFILTPICPQALTFRPLVMDARRRITIEI
                     VQANPGTAIAADGRVMPFDGRDRIAITRYSADFLLVRNPKRSEWSALRRKLRWGEGPN
                     VH"
     gene            3402..4049
                     /locus_tag="PLU99_10915"
     CDS             3402..4049
                     /locus_tag="PLU99_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOO17607.1"
                     /translation="MQTQPTASSKPASAKLRRLTTILLAIVAASSAGGALLWWHAGQW
                     PRRFAIVDAGRLYRSGEVTPQHLARLHHEYGIRRVISLLDPTAAVTCAEREAAQTLGL
                     EWHNVPLPGDGASTPRQRDLLRALLVQPDSPPTLVHCAAGVNRTGLAVGMYRLHCQGW
                     SLAQVMAELRANDFEDLPKHQNLRDALQAEAQLAEQAAAPAAHLPDAEVPGLPEP"
     gene            complement(4071..5069)
                     /locus_tag="PLU99_10920"
     CDS             complement(4071..5069)
                     /locus_tag="PLU99_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOO17608.1"
                     /translation="MAVTHVEVQQVGELVRQHHESFERLLAEIRRVIVGQDNLLKKML
                     VGLLADGHILLEGVPGLAKTTAVACLARAISTKFQRIQFTPDLLPADILGTMVFQPQQ
                     GTFAVKKGPIFSNLILADEVNRAPAKVQSALLEAMQERQVTIGGETFPLERPFLVLAT
                     QNPIEQEGTYPLPEAQVDRFMLKLLVDYPAAPEERQILDRMATHRSAPAVEPVMEPER
                     IFSARKVLDEIYMDDKIKDYIVALVQATRRPADFKLKIAEWIEYGASPRATIYLAMGA
                     RAMAFLAGRGYVTPQDVKDIAPDVLRHRIIVSYEAEAEEKTSEDVVRHVLDHVEVP"
     gene            complement(5303..5803)
                     /locus_tag="PLU99_10925"
     CDS             complement(5303..5803)
                     /locus_tag="PLU99_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOO17609.1"
                     /translation="MPMENLRDAFIHELEDILHAEKQLSKALPRFTKAATDEKLRDGF
                     QKHADETKNQISRLERIFQMLNVKPKNRKCDGMAGLIEEAATIMGEDADPEVMDALLV
                     EAAQKIEHYEIATYGTLCTWAEELGYQDVVDVLKETLREEKDEDRKLTEASRDLNIRA
                     AQPAGA"
     gene            6025..6684
                     /locus_tag="PLU99_10930"
     CDS             6025..6684
                     /locus_tag="PLU99_10930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO17610.1"
                     /translation="MPETPENVPVRCPATPAFLCLKRCIDAYLAFVALLAAAPLLLLI
                     AWLIRRESPGPALFRQLRAGRNGRPFTLLKFRTMRSDADPYGDSPQSGSDPRITRIGR
                     ILRETSLDELPQLINVLRGEMSLVGPRPLYLQQMREWNTRQKARLLVKPGLTGLAQVR
                     GRAALTIEDKIEWDVRYVEQISLKTDLAIIIETVAQVFRRGGIYEVQYSRTQARRTQR
                     D"
     gene            complement(6779..>7859)
                     /locus_tag="PLU99_10935"
     CDS             complement(6779..>7859)
                     /locus_tag="PLU99_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO17611.1"
                     /translation="SINRIANNTEFQGRKLLSGELAYTTSGVSDATDYFENVIINAAK
                     IPSEGARNVTVEVTGSAQLAEMVFAASSVTGGNVTIQIAGNLGSETLTFGSGTKISTM
                     RDAINASRDMTGVSAVINGSGTELQINSIEYGSDQFVTVKVLDGTLADLQPGDHGDNT
                     DFGQDASVKINGVTAITNGLRASMRSSALSLDIDLSADFGTLATADSEKVFAITGGGA
                     DFMISPTVSLAGQASMGIQAVDTGSLGNAGVGYLSSLATGQTNALSTGNYAQAQRIVR
                     AAQDLVSQLRGRLGAFQKNTLETTASSLAVTYENTAAAEASIRETDFASETATLTRSQ
                     ILVQSATNTLKLANAQPQTVLALLG"
BASE COUNT         1392 a         2559 c         2385 g         1523 t
ORIGIN      
        1 acgtggcctc gagcatgtcg gacttctgct tgcgctcctc gttctcgcgc tgcagccggt
       61 cctggagaac cttcagttca tcctgggccg atttcatccg ctcgtattcg ttgaataacc
      121 ggccgatgtc caccaccgcc acgcagcagc ccgtctgtgc tctcccctgc gcgtgcagcc
      181 cgccggccag cccgaagacg accagtgccg cgcccgcccc aaccaacaaa cctgtccgcc
      241 atcccttgct catgctcgac tccaagctcc aaaaagcgaa aaaaacgcgc ggccgcgccg
      301 ggacccgcgc cggcccctat tgtcgccggg ccttctgttg ccacaagagc gcgagttgaa
      361 cagatccgtt ggcgggtgtc ctggccggtg ctgaaacgaa cgcgatgcgc gcggaagcgc
      421 tgcccccaaa gtcgtgatat cacactgaaa cgagcctgat tggaacgcca ctcaggcggg
      481 cgtgagctcg cactttgagg cacatgccgc tttttccggc ggttgaacgg cgatggtgcc
      541 cggtttggtg accggcaccg tgccgtactt ggcggccact ttacgaatca tctcgtcgcg
      601 ataagtgctg gtccaactac acgccgggat catttttatc tgaccgtcgg aaacatcctc
      661 gtaggcgtag cacgagcggc agttctcgat gcgggccgcg tcgatctcgt gatattcctc
      721 aaacggtatg acggccacgc ggagaatccg cgagagattg aagtactgcc gggctaaatc
      781 gccggcgcgc cgcccgcgca gaaggccgcc caggaacttc agcaaaacca caaccgggtt
      841 cccgcggaac gccttgcgaa tagcaagccc acgcaccacc agcggcagga acatccgcgc
      901 cgccagccac ttggcacgcc agcggtcgat gcggctggcc gggttgagac gcgccacgat
      961 cggctgcgtc tgcttggcgc gcttgaatac ttcctgtgca acccggctga atggcatttt
     1021 caggaaatga ttgatgctga cgtagcgctg gccatccgaa gcgagcagcg tcatcgtttc
     1081 gcaatccggg tgtacgccgg cgaacatcag cgactccgac gtgctgcggc cggtcaggaa
     1141 tgtgtacagc ggccgcaact cctggatgaa gcctgccggg acgaactcca cctggccgcc
     1201 ggggatgctc tcttcgacca tcgcctcggc gtcctcacgc gtggtttgca cctgcgtctt
     1261 gaaccggccc tcctcccagg tttccgccag cggcagcagc gccagttgct caatcacgcg
     1321 attgttctcg tggcagcatt cgaccaaatc cgcgatccac ttgtcattta tgccgcgagc
     1381 tgcacagcac aggatggtgt gcttgatgcg gctatacttg gcgaggttcg ccagcccttt
     1441 tagcttcttt tcgcaagcac ccgggccgcc gcgcatgcac ccatagattt ccggactccg
     1501 cccatccagg ccgaaccgca aacgcacgcg ggcctcgcag agttccttgc agtattcctc
     1561 atccgccagg cgcaggccgt tggtgatgac gctcatcttg agcccatacg tgcggccgag
     1621 cttgaccatg tcgagcaggt cgtggcgcac ggtcggctcg ccgccgatga gctgcaccag
     1681 cggcttggga tcgaggtggc agagagcctg gaaaattttc tcgaagtagg ccagcggcgg
     1741 gttgtattca aaacccatac cgcggatgtt ggcgatgcag attgggcaac ccatgttgca
     1801 gcggttggtg atgtcgatca ggacgatcgt cggattatgc ggcttacggc actccgtgca
     1861 gcgcaggttg cagtttttct tcgggtcctc gtagccccag aaatcacgct tgcgttgcca
     1921 gcgcgcctca tcggaggcaa ccatcgattc gttatctccg caggtgggac agtgcttcac
     1981 gaggtacacg tgtccatcgt gtttgacgtg ttcgcacggt acgagcgtcc gacagtgctt
     2041 gcaaaatgcg cgattggcct gctccatgcc gactctccga ttggggcaac gcttcgggac
     2101 ggactgattg ccgcgccgcg tgaaatgata acgcatttcc gggcgcgcga agcggaagaa
     2161 atgcgccggc cgagattttc tcgaaaccaa aaccgcccct gccgctccga atttgtgcga
     2221 ccgccccgcg accggatgcg cggagccaac aatgcgcgtt atcgaatcaa aagtgtccgg
     2281 ttgcgccgta ggcgagcgga cgctggtaga tcggatgcgg ccgggccggc cttcgatctc
     2341 cgttttcaat cctcttttcc gtgacctgcg ttgccgcgct gccgcccgca gtctccttgt
     2401 gtacgccgtc gaacgggtca caatttgagc aaagggtact aaaatgaccc agtggccacg
     2461 cgccttttcg gcgttgggcc gttctgctgg cgccaggcga gcctggctgc tgccgtccga
     2521 tttcggccgg caggcccaaa gtgagcgctc catgaaacga gtggcaatcc tcgcctcgcc
     2581 cgacaaaccg gcggcggttc aaacgctcgg tcgaatgcag caatggctgc gcgggcgggc
     2641 acaggtggtt tttgccggac tgacatatga cgggcggcaa gctttgccgc accgccccga
     2701 gttgctcttc agtctcggcg gcgacggaac gctcatttcg gccgcccaca gcttgggcga
     2761 gcagcaactc ccgatcgtcg gcgtcaatct cggcaagctc ggctacctgg ccgagttcac
     2821 catggaggag ctcgagcggg aaggagactt tctcttcgac ggcgtgctgc cgctgacgcg
     2881 ccgcataatg atggaagtgg tcctggaacg ccgtgaacaa ccggtgttcc gcacgctcgc
     2941 gatcaacgat tgcgtggttc tggccgggcc gccattccac atgattgagg tggcggtcac
     3001 tgtggatggc gacgaggtca cccatgttcg cggcgatggt ctgatcgtcg ccacgccgtc
     3061 cggctcgacc gctcacaacc tggccgcggg cggcccgata ctcgaaccga ccggcgcggc
     3121 tttcatcctc acgcccattt gcccgcaggc gctgacgttc cgcccgctgg taatggacgc
     3181 gcgccgccga ataacgattg aaatcgttca ggcgaacccc ggcacggcga tcgccgctga
     3241 cgggcgcgtt atgccgttcg atggacgcga tcgcatcgcc atcacgcgct actcagccga
     3301 tttcctgctg gtgcgcaacc cgaagcgctc cgaatggtcc gccctgcggc gcaaactgcg
     3361 ctggggcgag gggcccaacg tgcactgaaa aacagccgcc aatgcaaacc caacctaccg
     3421 cgagctctaa accggcaagc gcgaaactgc gccgcctcac aaccatactc ctggcaattg
     3481 tggcggcttc cagcgccggc ggggcgctcc tgtggtggca tgccgggcaa tggccgcggc
     3541 gcttcgccat cgtcgatgcc ggccggctct accgcagcgg ggaggtcact ccgcagcacc
     3601 tggccagact ccaccacgag tacggcattc gccgcgtcat ctccctgctt gacccgaccg
     3661 ccgcagtcac gtgcgctgaa cgggaggccg cccagacgct cggcctcgaa tggcacaacg
     3721 tcccactgcc cggcgacggc gccagcactc cgcggcagcg cgacctgttg cgggcgctac
     3781 tcgtgcaacc cgattctcca ccaacgctcg tacattgcgc cgcgggtgtg aatcgcaccg
     3841 ggctggccgt cggaatgtac cggctacatt gccagggctg gtcgctcgcg caagttatgg
     3901 ccgaactacg cgctaacgat ttcgaagacc tgcccaagca ccaaaatctg cgcgacgccc
     3961 tgcaggctga ggcgcagctt gccgagcagg cggcggctcc tgccgcgcac cttccggacg
     4021 ccgaagtccc tggattgcct gaaccctgaa ttcggcctga gacgccccgc tcagggcact
     4081 tctacgtggt cgagaacgtg ccgcacaacg tcctcgctgg ttttttcttc ggcctcggcc
     4141 tcgtaactga cgatgatccg gtgccgcaga acgtcggggg cgatgtcttt tacgtcctgc
     4201 ggcgtcacgt agccgcggcc ggccaaaaag gccatggccc gcgcgcccat cgccagatag
     4261 atcgtggcgc gcggcgaagc gccgtactcg atccactcgg cgatcttgag cttgaaatcc
     4321 gccgggcgcc gcgtggcctg caccagggcc acgatgtagt ccttgatctt gtcatccatg
     4381 taaatttcgt cgagcacctt gcgggcggag aagatgcgct caggctccat gaccggctcg
     4441 accgccggtg cgctgcggtg cgtcgccatc cggtcgagta tctggcgctc ttcgggcgcc
     4501 gccggatagt cgaccaacag cttcaacatg aagcggtcca cctgggcttc gggcaacggg
     4561 taggtgccct cctgctcaat cgggttctgt gttgccagaa ccaggaacgg acgttcgagc
     4621 ggaaacgttt cgccgccaat cgtgacctgc cgttcctgca tggcttccag cagcgcgctt
     4681 tgcaccttgg ccggtgcgcg gttgacctcg tcggccaaaa tcaggttcga gaaaatcggc
     4741 cccttcttaa cggcgaaagt gccctgctgc ggctgaaaga ccatcgtgcc gagaatatcg
     4801 gccggcagca gatcaggcgt aaactggatg cgctggaact tggtgctaat ggcccgcgcg
     4861 agacacgcga ccgcggtcgt tttggccagg cccggcacgc cttcaagcaa aatatggccg
     4921 tccgcgagca acccgaccag cattttcttc aacaggttat cctgccccac gatcacccgc
     4981 cggatctccg ccagcagcct ttcgaagctt tcatggtgct gacggaccaa ttcgcccacc
     5041 tgttgaacct caacgtgcgt aacagccatt tgctctcctg tcgcggcccg caggctaccg
     5101 tgcgcggctg accgaaaaaa ggtaatccgg gcgctgagaa aatcaaatcg gcggtcgatt
     5161 gaggtcgccg cctgctgttg cgttagacga ttggcgaagg gaaaaccgac aaaaaaagcc
     5221 ggcgcacgcc gtgcgccggc ctttgtgagg aggttctgtt aagtgcgccg ctcggttctg
     5281 gccgcacgct ggggcggccc gcttacgcac ccgcgggttg ggccgctcgg atattcaggt
     5341 cgcgcgacgc ctcggtgagc ttgcggtctt cgtctttttc ctcgcgcaag gtttccttca
     5401 gcacatcgac gacgtcctgg tacccgagtt cctcggccca cgtacagagc gttccatagg
     5461 tggcgatctc gtagtgctcg attttctggg cggcctcgac tagcagggcg tccatgactt
     5521 ctggatcggc gtcctcgccc atgatggtcg cggcttcctc aatcagcccg gccatcccgt
     5581 cgcatttgcg attttttggc ttgacgttaa gcatctgaaa gatgcgttcg agccgcgaga
     5641 tttggttctt ggtttcgtcg gcgtgctttt gaaatccgtc ccgcaacttc tcgtccgtgg
     5701 cggccttagt gaaccgcggc agcgccttcg aaagctgctt ttccgcgtgc aaaatgtctt
     5761 ccaattcatg tatgaatgcg tcgcgaaggt tttccatcgg catggctgta ctcctcggtc
     5821 tgataccgaa ttcccatgat tcaacggccc ggattattcc cgccacggct gacgaggcac
     5881 tgaattccgc acgaagcacg cgtaacgccc ccaaacctcg ccccgcagcg atgttttgag
     5941 ccgctcacgc caccgcatcg ccaggaaatg tgccccggca gctcaagccg tagcacgttt
     6001 catcgcaccc gataaactgc ccgcatgccg gaaacccccg aaaacgtgcc ggtgcgttgt
     6061 cccgcgacgc ctgcgttcct ctgcctcaaa cgttgcatcg acgcctacct cgccttcgtt
     6121 gcgcttctcg ccgcagcccc cctcctgctt ctgatcgcct ggctcatccg ccgcgagagt
     6181 cccggccctg cgctcttccg ccaactgcgg gccggccgca acggccgccc gttcacgctg
     6241 ctaaagtttc gcaccatgcg cagcgacgcc gacccgtacg gcgactcgcc gcaaagcggc
     6301 agcgacccac gcatcacccg cattgggcgc atcctgcgcg aaaccagcct ggatgaactg
     6361 ccgcaactca tcaacgttct ccgcggcgaa atgtcgctgg tcggcccgcg tccgctctat
     6421 ctgcagcaaa tgcgcgaatg gaatacccgc caaaaagcgc gtctgctggt caagccgggc
     6481 ctgaccgggc tggcccaagt ccgcggccga gccgcgctga cgattgagga caaaatcgaa
     6541 tgggacgtcc gctacgtcga gcagatcagt ctaaagaccg acttggccat cattatcgag
     6601 acagtggccc aggtctttcg ccgcggcggc atctatgagg ttcagtactc gcggacgcag
     6661 gcgcgccgca cgcagcgcga ttaaaaaggg cggcagcgcg cctggtgggg cacgctgccg
     6721 ttcatgagcc ggaaggggag gaactcgctt gttggctgct actgccgagg gcagccgctt
     6781 aacctagcag cgccagcacg gtctggggct gagcattggc cagcttcagc gtgttggtgg
     6841 ccgactggac caggatctgc gatctggtca gcgtggcagt ctccgaggcg aagtccgttt
     6901 cccggatgga ggcttcagcc gcggccgtgt tctcgtacgt taccgccagc gagctagcag
     6961 ttgtttcgag cgtgttcttc tggaacgcgc ccaggcggcc gcgcaactgc gaaaccaggt
     7021 cctgcgccgc gcggacgatg cgctgtgcct gagcgtagtt gccggtcgag agggcgttgg
     7081 tttggccggt tgcgagggac gagaggtagc ccaccccggc gtttccgagc gagccggtgt
     7141 ccacggcctg gatacccatg ctggcctggc cggcaagcga cacggtcggc gagatcatga
     7201 agtcggcgcc gccgccggtg atggcgaaga ccttttcgga atccgcggta gccagggtgc
     7261 cgaagtcggc cgacaggtcg atgtccaggc tcagggcgga actgcgcatg ctggctcgca
     7321 ggccgttggt gatcgcggtc acgccgttga tcttgaccga ggcgtcctgg ccgaagtcgg
     7381 tgttgtcgcc gtggtcgccg ggctgcaggt ctgcgagggt gccatcgagc actttaaccg
     7441 tgacaaactg gtcggagccg tactcaatgc tgttgatttg cagctccgtt cccgagccgt
     7501 tgataacggc cgagaccccg gtcatgtccc ggctggcgtt gatcgcatcg cgcatggtgg
     7561 agattttcgt gccgctgccg aaggtcaggg tttcgctgcc caaattgccg gcaatctgga
     7621 tcgtgacgtt accgcccgtc acggaggacg cggcgaaaac catctccgcc aattgggccg
     7681 aaccggtcac ttcgaccgtg acgttgcgcg cgccttcgga cgggatcttg gccgcgttga
     7741 tgatcacgtt ctcgaagtaa tccgtcgcgt cggacacgcc cgaagtcgtg tacgccagct
     7801 cgccggaaag cagcttgcgg ccctggaact cggtgttgtt cgcgatacgg ttgatcgag
//
LOCUS       DAOSYS010000172         7849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7905_length_7849_cov_5.635489, whole genome shotgun sequence.
ACCESSION   DAOSYS010000172 DAOSYS010000000
VERSION     DAOSYS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_7849_cov_5.635489"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLU99_10940"
     CDS             complement(<1..224)
                     /locus_tag="PLU99_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17612.1"
                     /translation="MSIITTARESAAARPTAPVVIEEAAATHAAGAWARYVRGMPSGT
                     VFHDPAWCAAVERTFGHRPLHRVALRGGRVV"
     gene            complement(199..1107)
                     /locus_tag="PLU99_10945"
     CDS             complement(199..1107)
                     /locus_tag="PLU99_10945"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOO17613.1"
                     /translation="MGSMLEIMKKVRDEGDAPDPAVEGVVELMPEEAARGGPAAADSR
                     FTQSEPGAVSDLVAEAASVAFPSTQEWDAKLIDPAVVAFHARYSVECEQFRSIRARLL
                     SMNSGGDPCVLAITSSVPGEGKSTCTLNLGMVMAEGGEQRTLIVDADLRRASITRMLA
                     LDGQSGLSDLLRGTGTVKQHVYPTPYENLKVLPAGAACGAGQSELLGSARLREVFAEL
                     RAAFDYVFVDMPPVSTVADASVLGPRCDAALLILQMRRTPEPVAQQAVRTLQANKVRV
                     LGCILTRHHERHAHMYDINEYYNYGS"
     gene            complement(1133..2851)
                     /locus_tag="PLU99_10950"
     CDS             complement(1133..2851)
                     /locus_tag="PLU99_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17614.1"
                     /translation="MTHDRPMEDVRNTIGEYARLVRNRWRPALVGLALVASTAFWCSQ
                     WLPRRFAASTIFERRDDVVLRNLISKNSPYSFESLKSTLVMDMTGSRAMANAAVTLGI
                     LAPEAVPAEGPLTEAGLRALDEALAAHGLRASVQMLQSTPSLDTIKLTCEAHDPDMAQ
                     RCVVALRDGYISHTRDRITDVLRSTRRFFQTEVDRLQQQLSSTNQALAEPFKDFPGLD
                     PNDTAGAGHRLEVLRSERERLLERKTELEAQVAVHEQYAALIADQPAGAGATEAFQRS
                     VRAAMAKIDQEMGEAMALRRMTAEHPEVRGLQAKRAALSALLEELVRQSSAEALAHAG
                     DGWAVSAPDGERNAQQLRAKLELEALQRQLAAVQAKFEQADERVNRFGTLFERLLKHG
                     AGLRDLTDQAATQADTLATWREHLLQLDRIMAADSEQRGTSFAVLEEAKNSDRPVSPR
                     GKAVMAVCTASGLAGAALLIGILELLDRSFRSAGQVARALGIPVLESIGVIPTPQVRR
                     KRWLCRLLWAPTLTLLLAVLLTTGALAYFSLEHPRAYDSALTNLRDAVPRYLGLGGNC
                     GTSVRP"
     gene            complement(2869..3684)
                     /locus_tag="PLU99_10955"
     CDS             complement(2869..3684)
                     /locus_tag="PLU99_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOO17615.1"
                     /translation="MKDAWKTIETRSRRRAEALALCGCILLATSAGCQSRHAHHVAFL
                     RADEQIVSTGEYMVAPPDALLIHAPVAPEIDGTTEQVRPDGKIALRLLGEVDVAGLTT
                     DQIAAKLRDLLARYYVEPEVVVDVHRYASQYYYVFGQVRRPGPKLYTGRDTLLHALSE
                     AEPTFLAAKSLIRVVRPGTEDEASRVITINLDKMVKDGRANDNILLQPGDVIEVPPTA
                     LAWVGLRVRELLYPIEPIVDTYNAPAETIHSTHVYEGHYYDYRDDGRGRRRWR"
     gene            3889..>4830
                     /locus_tag="PLU99_10960"
     CDS             3889..>4830
                     /locus_tag="PLU99_10960"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO17616.1"
                     /translation="MQPFQHFQLKRPPFEAAPDPRAFCATEPHAEALATLEYALYSRK
                     PCTVLVGESGTGKTLLARMMARTASLQATILWLPGLGQPQTHSEIYIFPRGTLCGSDC
                     NQPPLLANFSDWIREHSRSSPSTVLIVDNADCLPSHGWRDVLILLSREVLFSEPVRIG
                     LFGLPSLLRRLASPELVQVRRRIFRTAVLQPLTRRDMCVYLSGRLVAAGGRLEDLFTS
                     EALDQMHRLTRGIPASLNQLCENAMLEAMSAGRTRITAADILAAAQAVAGPRPPLHTV
                     WKSVKLLNRPVLQPPAPQASAAARLSRHADHSGGDWAG"
     assembly_gap    4831..4840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5159..6988)
                     /locus_tag="PLU99_10965"
     CDS             complement(5159..6988)
                     /locus_tag="PLU99_10965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOO17617.1"
                     /translation="MPGGLVQLYRVGADATLGIGLSKERWRNVSAAALPGPRRSAGVQ
                     LNVRGVTTDCSAISSDALAGRTMRKIGTAQRPVLEEAGCKMAILAGSRIAANAGDVSS
                     AAPCGSAARPVREPRSLTILPRPESGRANVGRFRQAGGAESPVKVLLRHLEPITWAYF
                     DFLLVNGFTWLWYVLMVSGRPTYAWVFGPWMTGLIFGSCITLAGLVFGLYERKTLCAR
                     TRILVRSALTVGAGLVLAYACIFLFFYGSASRWLGLAVGAMYCATALPLRLVAHGAVV
                     SRSRRALCVGYEASIRKLAAFLERAVRPPVRIVGYVDIARESGRSGSGGAGCSPEEGG
                     ARLPRLGRIEDIGTVLVRHQIDEVIVGSEVTARPDVGHAVLACLDHRCRVTDQPTFVE
                     KLLGEVPAENIDAQWFLLADVQGSGGYETAKRVVDVVVAVVGLAVALLLWLPIVLAIR
                     LESRGPALFRQKRVGLHGREFTLFKFRTMRLDAEQQGARWAERNDPRVTRVGRWLRLT
                     RLDELPQLWNILRGEMSIVGPRPERPEFVEQLSRVIPNYRQRHLIKPGLTGWAQIHLG
                     YGATVDDARRKLGFDLYYLKHRSIDLDLSILIRTLGTFLLGAR"
     gene            7161..>7849
                     /locus_tag="PLU99_10970"
     CDS             7161..>7849
                     /locus_tag="PLU99_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17618.1"
                     /translation="MNCGCRSAEPVCQPQAAATVLAAGTDDRDNCRTIFADNACGVRF
                     ATTLVIAELAGDDSPDALPGDQAEIADSHQTLPPLPVRPESSGDDALCPAGETPACGA
                     DEGLKVVVISPAGQGRWVEAFRGLSFIRDLRFLSPIDLKSQGGGLPGILAAARRAQGG
                     LLLTYTANRCGPNSERIAGTIYAVDSGRPLVQLQSYMQFLNEEGLECAVDEERGDHRD
                     QDAIYQTAREF"
BASE COUNT         1374 a         2864 c         2191 g         1410 t
ORIGIN      
        1 accactcttc cgccacgcag cgccacacgg tgcaacggcc gatggccgaa cgtccgttca
       61 accgcggcgc accaggccgg gtcgtgaaac accgtgccgc tcggcatccc gcgcacgtac
      121 ctggcccacg cgccggccgc atgtgtggct gcggcttcct ctatcaccac cggcgccgtt
      181 ggccgagcgg cggcgctttc acgagccgta gttataatac tcattgatgt catacatgtg
      241 tgcgtggcgc tcatgatgcc gcgtgagaat gcaccccagg acgcgcacct tgttggcctg
      301 caatgtccgc accgcctgct gcgccaccgg ctcaggtgtc cgccgcatct gcaaaatcag
      361 cagcgcagcg tcgcagcgcg gccccagcac gctggcgtcg gccaccgtgc tcacgggcgg
      421 catgtctacg aatacatagt caaacgccgc gcgcaactcc gcgaatacct cccgcagacg
      481 cgccgaacca agcagctcgg actggcccgc cccgcaagcc gccccggccg gcaatacctt
      541 caagttttca tagggagttg ggtagacgtg ttgttttact gtaccggtgc cgcgcagcag
      601 atcggatagg ccactctgcc catccagggc cagcatgcga gtgatcgagg cgcgccgcag
      661 atcggcatcc acgatcaatg tccgctgctc gccgccctcc gccatcacca ttccaagatt
      721 cagcgtgcac gtgctcttgc cttcgcccgg cacgctgctg gttatcgcca agacgcacgg
      781 atccccgccg ctgttcatgc tcagcagccg cgcacgtata gatctgaact gctcgcattc
      841 caccgaatac cgggcgtgga acgccaccac cgccggatca atcagcttcg catcccattc
      901 ctgcgtgctc ggaaaggcga cgctcgccgc ctcggccacc aaatcgctca ccgccccagg
      961 ttcagattgt gtgaaccggc tgtcggccgc cgcgggcccg ccgcgcgccg cctcctccgg
     1021 catgagctcg acgacgccct ccacggcggg gtccggtgcg tccccttcgt cccgaacctt
     1081 tttcatgatt tccagcatcg aacccatgct ttgccttcca gcaccgaact tctcaaggtc
     1141 gcactgacgt gccgcagttg ccgcccagtc cgaggtaacg cggaacggcg tcgcgcaggt
     1201 tcgtcagggc gctgtcatag gcgcgcggat gctccaggct gaaataggcc agcgcgccgg
     1261 tcgtcagcag cacagccagc aagagcgtta acgttggagc ccacaacagc cggcacagcc
     1321 agcgcttgcg cctcacctgc ggcgtcggaa tcactccgat ggactccaga accggaatgc
     1381 ccaaggcgcg cgctacttga cccgcggagc ggaacgagcg gtccagcagc tccagtatcc
     1441 cgatcagcag ggccgcgccc gctagccccg acgcggtgca taccgccatg acggctttgc
     1501 cgcggggcga caccgggcgg tccgaattct tggcttcctc cagcactgcg aatgacgtgc
     1561 cgcgctgctc gctgtccgcc gccataatgc ggtccaattg cagtaaatgc tcgcgccacg
     1621 ttgccagcgt gtccgcctgc gtggctgcct gatccgtcag gtcgcgcaag cccgcgccat
     1681 gcttgagcaa acgctcgaac agtgtgccga aacgattaac gcgctcatca gcctgctcga
     1741 acttggcctg tactgccgcc agttgccgct gcagggcctc cagttccaat ttggcgcgca
     1801 actgctgggc gttgcgctcg ccatccggcg ctgacactgc ccagccatcc ccagcatgcg
     1861 cgagggcctc cgcgctggat tgtcgcacta actcctccag cagcgcgctc aacgccgcac
     1921 gtttggcttg cagcccgcgc acctccgggt gctcggccgt catgcgccgc agcgccatgg
     1981 cctcgcccat ctcctggtcg atcttcgcca tggccgcccg cacgctgcgc tgaaatgctt
     2041 ccgtcgcccc ggcccccgcc ggctggtccg ctatcagggc cgcgtattgc tcgtgcaccg
     2101 cgacctgtgc ttccaattca gtcttgcgct ccagcaggcg ctcgcgttcg ctccgtagca
     2161 cctccagccg gtgacccgcg cccgccgtgt cgttcggatc caaacccgga aaatccttga
     2221 atggctcggc caaagcctgg ttcgtactgg atagctgctg ctgcaagcgg tccacttcgg
     2281 tttggaagaa acgccgcgtg ctccgcaata cgtcagttat ccggtcgcgc gtgtgcgaga
     2341 tatacccgtc gcgcagagcg actacgcagc gctgcgccat gtccggatca tgcgcctcgc
     2401 acgtcagctt gatggtgtcc aggctcggtg tgctctggag catctgaact gacgcccgca
     2461 atccgtgggc cgccagtgcc tcatccagcg cacgcaaacc cgcctcggtc agcggaccct
     2521 ccgccggaac ggcttcgggc gcgagaatcc ccagcgtcac agccgcgttc gccatggccc
     2581 gcgagccggt catatccatg accagtgtgg atttgagact ttcaaagcta tagggcgagt
     2641 tcttgctgat tagattccgc agcacgacgt cgtcgcgccg ctcgaaaatc gtgctggccg
     2701 cgaagcgccg cggcaaccac tggctacacc agaacgcggt cgatgccacc agcgccaaac
     2761 ccaccaaagc cggacgccag cgattccgta ccaggcgcgc atactcaccg atggtgttgc
     2821 gaacatcctc catcggtcgg tcgtgcgtca tattcgcctc tgtttcactc agcgccagcg
     2881 gcgccgcccg cggccatcat cacggtagtc gtagtagtgg ccttcgtaga cgtgcgtgga
     2941 gtgaatcgtc tcggccggcg cattgtaagt gtccacgatc ggctcgattg ggtacagcaa
     3001 ctcgcgcacc cgcaacccta cccacgccag tgccgtcggc ggaacctcga tcacgtcgcc
     3061 gggctgcagc agaatgttgt cattcgcacg cccatctttg accatcttgt ctagattgat
     3121 ggtaataact ctgctcgctt catcctccgt cccgggccgc accacccgaa tcagcgactt
     3181 cgccgcgagg aacgtcggtt cggcctccga caacgcgtgc aacaacgtgt cgcgccccgt
     3241 gtacagcttg ggacccggcc tgcgaacttg gccgaacaca tagtagtact ggctcgcata
     3301 gcggtgcacg tccaccacca cttccggttc aacgtaatac cgggccaaca ggtcacgcag
     3361 cttggccgca atctgatccg tggtcagtcc cgcgacgtcc acttctccca gcagccgcaa
     3421 cgcaatcttg ccgtccgggc gaacttgctc cgtcgtaccg tcaatttccg gggccaccgg
     3481 ggcgtggatg agcaatgcgt ccggcggcgc taccatgtac tcgcccgtcg aaacaatttg
     3541 ctcgtccgcc cgcaagaacg ccacgtggtg ggcgtgccgc gactggcacc cggcgctggt
     3601 cgcgagcaat atgcagccac ataacgccag cgcttccgct ctacgtcgtg accgcgtctc
     3661 gattgtcttc cacgcgtcct tcatgtccgg ccgaatcttc ctttcttgtt caagatgccc
     3721 ggaccaggcc acatctgcca agcggcccgt ctcgtgacta atcgtccaaa acccccacag
     3781 ggtgtaatac cttataagaa ccgtccacaa actttcggga ccccccttta tttcagcgct
     3841 ttagacggac ttccgatgtt ctttgcggct gataaaccgg agcccggctt gcaacctttc
     3901 cagcacttcc aactgaagcg cccgcccttt gaggccgcgc ccgatccccg cgcgttctgc
     3961 gccaccgaac cgcacgccga ggccctggca actctggaat acgccctcta ttcgcgtaag
     4021 ccctgtaccg tgctggtcgg tgagtccggg accggtaaaa cgctcctggc ccgcatgatg
     4081 gcccgcaccg cctcgctcca ggccaccatt ttatggctcc ctggtctggg gcagccgcag
     4141 acacattccg aaatctacat cttcccccgc ggcacactgt gcgggtctga ctgcaaccag
     4201 cctccgctgc tcgcgaattt ctcggactgg attcgcgaac acagccgctc cagcccttcc
     4261 acagtcctga tcgtcgacaa cgccgattgc ttgccctcac acggctggcg tgatgtcctg
     4321 attttgctct cccgcgaagt gctcttttcc gaaccagtgc gaatcggcct gttcggcctg
     4381 cccagcctgc tgcgccgtct ggccagcccg gaactggtgc aagtccgccg ccgcattttc
     4441 cgcaccgccg tcctgcaacc cctcacacgc cgcgacatgt gcgtgtacct tagtggccgc
     4501 ctggtggccg ccggcggccg tttggaagac ctcttcacct ccgaggcgct cgaccagatg
     4561 caccggttga cacgcggcat ccctgccagt ctcaatcagc tctgcgaaaa cgccatgctg
     4621 gaggccatga gcgccggacg gacacgcatc accgctgcgg acatactggc cgcggctcaa
     4681 gccgttgccg gcccgcgacc gcccctgcac accgtgtgga agagcgtcaa gctcctcaat
     4741 cggccggttt tgcagccccc cgccccgcaa gcgtccgcgg ctgctcggct ttctcggcac
     4801 gctgatcaca gcggcggcga ttgggctggc nnnnnnnnnn ccggctttgc agccccccga
     4861 cccgcaagcc tctgcggccg cggccgctcg gctctcccgg acacagccac tcaccggcct
     4921 cagcggagct ccgcgcgtga ccggcttacc ctctagaggc ggtctttaca cgccgcgtca
     4981 cctggccgat tcgagcgttc cgccacattt cacgcagcaa ctgcattacc tcgaaaaccg
     5041 cttgcacatg gtgctctcgt cgatgcagcg cgcttgcgcc gttacggatg ccgccttagc
     5101 gcggacgcac caaattggaa gccccctttc ttctgctccc gccgcccccg cgttggattc
     5161 aacgcgcccc gagcaggaac gtccctaacg tgcggatcaa aatcgaaaga tccaagtcaa
     5221 tcgaacggtg cttcaagtag tacaagtcaa agcccaactt ccgccgtgca tcatcaaccg
     5281 tcgccccgta accaaggtga atctgcgccc agccggtcag ccctggcttg atcaggtgcc
     5341 tttgccggta attcggaatc acccggctta gctgctccac gaattccggc cgctccggcc
     5401 gtggcccaac gatcgacatt tcgccgcgca gtatgttcca gagctgcggc agctcgtcga
     5461 gccgcgtcag ccgcagccac cgcccgaccc gcgtcacacg cggatcattg cgctccgccc
     5521 agcgcgcccc ctgttgctcc gcatccagcc gcatggtccg gaacttgaat aacgtgaact
     5581 cccgtccatg tagccccacg cgcttttgcc ggaacagcgc cggtccgcgg ctttccagcc
     5641 gaatcgccag cacaatcggc agccacaaaa gcagcgccac tgccagcccc accacggcga
     5701 ccactacgtc gactacccgt ttggccgtct cataaccgcc gctgccctgc acgtccgcca
     5761 gcaggaacca ctgcgcatcg atgttctcgg ccggcacctc tcccagcagc ttctctacaa
     5821 acgtgggctg gtcggtaacc cggcagcgat gatccaaaca cgccagcacc gcgtgtccca
     5881 cgtccggccg cgccgtcacc tcgctgccga cgatcacttc gtcgatctga tggcggacca
     5941 gcaccgtgcc gatgtcctcg attctgccca aacgcggcaa gcgcgccccg ccctcctctg
     6001 ggctgcatcc agccccgccg ctcccgctcc ggcccgactc acgcgctatg tccacgtatc
     6061 ccacaattcg caccggggga cgcacggcgc gctccaggaa cgccgccagc ttccgaatcg
     6121 acgcctcgta ccccacgcac aaagcccgcc ggctgcggct cacgactgcc ccatgcgcaa
     6181 ccagccgcaa tggcaacgcc gtcgcacagt acatagcgcc gaccgccaaa cccaaccagc
     6241 ggctcgcact gccgtagaag aacaggaaaa tgcacgcata ggccagaacc aatccggctc
     6301 caaccgtaag cgccgagcgc accagaattc gagttcgcgc gcacagcgtt ttgcgctcgt
     6361 aaagtccgaa aaccagcccc gccagcgtga tgcagcttcc gaagatcagc ccggtcatcc
     6421 acggtccgaa tacccaagca tatgtgggcc tccccgagac catcagcaca taccagagcc
     6481 aggtgaaccc gttcaccagt agaaaatcga agtacgccca ggtgatcggc tccaggtgcc
     6541 gcagcaacac ctttaccgga ctctcggccc cgcccgcctg ccgaaatctg cccacattgg
     6601 cccgccccga ctccggtcgc ggcaggattg tcaggctgcg cggttctcgc accggccgcg
     6661 ccgcactgcc gcacggcgcc gccgatgaca catcccctgc gtttgccgca attcgcgagc
     6721 cggccaatat ggccatcttg catcccgctt cttcaagcac cggacgctgc gccgtgccga
     6781 tcttgcgcat cgttcgaccg gccaaagcgt ccgaagatat agcgctgcaa tcggttgtta
     6841 cgccgcgcac attcagttgg acgccggcac tgcgccgtgg gcctggcagc gcggccgccg
     6901 agacgttcct ccagcgctcc ttgctgagcc cgattcccaa agtcgcgtcc gcgccgaccc
     6961 gataaagctg aacgaggccg cccggcatgc tccgcggcgc aaacctgcac ggagaattag
     7021 tcgtgtcacg cttggttgat atcgctcatc acgctgcgcg cttagtagcg aagactcgca
     7081 gccccgagcg tggctccgcg cgccgcgctg gccgccggcc cgccgtcagc ctgctcggta
     7141 cctgcgtact agtgtttgct atgaactgtg gctgtcgctc cgctgagccg gtttgccaac
     7201 cgcaagccgc ggccacagtc ctggcggccg gtacggatga tcgcgacaac tgtcgcacga
     7261 tcttcgcgga taacgcgtgc ggagtgcggt tcgccacgac gctggtgatc gccgagttgg
     7321 ccggcgacga tagtcccgac gcgttgcccg gcgaccaggc cgaaatcgcg gacagccacc
     7381 agaccttgcc cccactgccg gtgcgacccg agtcctccgg tgatgatgcg ctctgcccgg
     7441 ccggtgaaac gccggcctgt ggcgccgatg agggcctgaa ggtggtagtc atatcgccgg
     7501 ccggccaggg gcggtgggtc gaggcctttc gtggcctgag tttcattcgc gatctgcggt
     7561 ttctttcgcc catcgatttg aaatcccaag ggggcggtct gcccggcata ctcgctgccg
     7621 cgcggcgggc gcagggcggg ctgttgctca cctatactgc gaaccgctgt ggaccgaatt
     7681 ccgagcgcat tgcggggact atctacgcgg tcgactccgg ccggccgctt gtgcaactgc
     7741 agtcatatat gcagttcttg aacgaggagg gcctggaatg cgccgtcgat gaggagcgcg
     7801 gcgatcaccg cgaccaggac gcgatttatc agacagcccg cgagttcga
//
LOCUS       DAOSYS010000173         7789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_7984_length_7789_cov_4.821826, whole genome shotgun sequence.
ACCESSION   DAOSYS010000173 DAOSYS010000000
VERSION     DAOSYS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_7789_cov_4.821826"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLU99_10975"
     CDS             <1..354
                     /locus_tag="PLU99_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO17619.1"
                     /translation="GGVEAVVHQAFGYVFGHYGEKATLALKYLVTQTSFGAGKVWDGS
                     LATLETVAGIPRTDAFATLQQVLGLDGQQATALLWDTYNPQYIWFPFAAIGVVAAIAL
                     AIFGQLAKRWRDMNA"
     gene            530..970
                     /gene="rplM"
                     /locus_tag="PLU99_10980"
     CDS             530..970
                     /gene="rplM"
                     /locus_tag="PLU99_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOO17620.1"
                     /translation="MKTANKCFLAKPGEVDKKWFVVDAQDKVLGRLATRIATVLMGKH
                     KPTYTPHVDTGDYVIVLNAGKVRVSGRKRQTEVYQRYTRYPSGRRVVSMEQMLARHPE
                     RVLREAVRRMLPKSKLGVHMLDKLKLHVGSTHPHQAQKPEPMPL"
     gene            1165..1542
                     /gene="rpsI"
                     /locus_tag="PLU99_10985"
                     /pseudo
     CDS             1165..1542
                     /gene="rpsI"
                     /locus_tag="PLU99_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(1913..2431)
                     /locus_tag="PLU99_10990"
     CDS             complement(1913..2431)
                     /locus_tag="PLU99_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17621.1"
                     /translation="MKRFAMAGFFTCVLFLTGCGDITGKWTLDCIEPESERQNFDVKC
                     LVLRDDNTFLLKACEDAKPAEVCGTYCYDKDTDTLTFTPEGGPARTYIAEVGLLGFGK
                     MKVCPTGEKAWTAYLKRCKCEPCADKCKPCPAKCKHNPDKCDKPCPKPCDKPAEKKAE
                     CPMHRGEPVQKN"
     gene            complement(2571..3626)
                     /locus_tag="PLU99_10995"
     CDS             complement(2571..3626)
                     /locus_tag="PLU99_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HOO17622.1"
                     /translation="MHVLPPYRRILLLTEGQLGAFTSKTAAALLRYRAPDVVGVLDSH
                     AAGTDVRTVFPWSPPVPIVADIAAARTLAPDALFVGIAPPGGALPGPMRQHIAHALAA
                     GMDVVSGLHQFLADDAELAALAQQNRARIFDLRRPPAERTIATARALSTRCRRVLTVG
                     TDGNVGKMVAGLELTAAARQRGLRAEFLATGQTGTMIAGRGVAIDAVVSDFAPGAVEE
                     LVLSAADSDICIIEGQGSIAHPGFSAVTLALLHGACPDAMILVHHARRTHYSAAPHMR
                     IPRLAELRAIYESAASLLHPSRVVGVALNPLGCDPKLVREETRLLAQELRIPVCDAIC
                     GCTALLDAVLANTIPKC"
     gene            3738..6050
                     /locus_tag="PLU99_11000"
     CDS             3738..6050
                     /locus_tag="PLU99_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17623.1"
                     /translation="MRKQRALVALAIVFLPGVMLAPLWRLGGLGAGEDDLLYYFPSRS
                     FFHDTVRAGQWPWINPWTGLDRPFAADPQSALWYPGTWLFALLPPLPAYAVSLWAHYS
                     LAIWGMYRLLRAGRLARPAALFGGLLFAFSGFMLAHRAHFSLQHAAAWAPWVFWRIQR
                     YAQNGGGFRLTTASIVAALQLYAGHVQIAAITALGSLVFIVAAGWAAAPKFTVKLRAA
                     VPAFGRWLGIWVLAGGLFAVQLVPTVMWPEQCTRVDRTYRDFVENSWYPWSAIGWVAP
                     MFFGQRTPNFFDQPYWGPSHQCEQFAYAGIVPLILAAVALRVGWRGDPQRRPWMVLLI
                     FGVLVALGQFGPLCPLLYQIPGSSLFRVPARALLLFNMSIAALAAISLHDLGAAHNPR
                     RVRLRAALQAWSARPHIKGAALVGMPLIVGAAVIPFLPSDWRAAALHALRPWSPSVFV
                     PLAVAMASFASLGVAARRWHRPRWLWLPVIVTTLDLAVIGWTIDIPLGKNGPADLLPQ
                     SEAPWVERVRDSGRRLWVVAHTADIYGEPLNILAANVNSLVGIQSLTDYGPLQPRSLQ
                     PRFAFRPWGESDQVRALLQDPRWMRTYNVGWILTRDPADEPAGCTLEEVDAAGWRLYR
                     NPSAAGMAVLEDAAAPVAISSQVHSNQRFEVVVDSWLAGERHRPGRTEAGQMQEKRLI
                     VSRLAQPGWQARIANRQVAIEPRDGLMSLRVPAGEYVKIDFSYSPPGLREGLLVSGAT
                     VIVILVLAAPGSRRTRQGQEATASSAVCTA"
     gene            complement(6027..6851)
                     /locus_tag="PLU99_11005"
     CDS             complement(6027..6851)
                     /locus_tag="PLU99_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO17624.1"
                     /translation="MASPSSPKPTTPAGATAGPVSPDSSDSAQRTIASLEQELIACQH
                     LVALGNIAAGIAHEVNNLMTPLLARAEFALASGSPAEMRKALERTKVQVERAVSITQR
                     MMDWAHNRECQLESCNVLEAVNEALATVVRPLEKDGIALTVDVPPDLHVRARRDLLIQ
                     VVLNLILNARNAMVHTPGSLKVTGWRNGTDVVLEFCDGGSGLTQEQIDRVLNPFLAGT
                     ECRGREWQGIGLGLNVCRIIVHKCGARIAVRANETRGCTVALRWPAETPSGCADGA"
     gene            complement(7037..>7789)
                     /locus_tag="PLU99_11010"
     CDS             complement(7037..>7789)
                     /locus_tag="PLU99_11010"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOO17625.1"
                     /translation="QWRAAPLRADDLRCWNALLDTPGFGPVIAHATYLINLASADKIV
                     RTRSLAAFEQELQRCQTLHIPYLVVHPGAATDGDRRSAIARVAAALDDLAKRGVAPQV
                     TPLLENTAGQGTALGWRLEELGEIIGRLRRSRAGVCLDTCHLFAAGYDIRRPQGYQEM
                     VASAERSFGLERIHCWHLNDSRAPLGSRVDRHEHIGRGRIGASGFRTLLSDSRFCEIP
                     MIIETPKGQDGAGRDWDWLNLRRLRRLAARRA"
BASE COUNT         1336 a         2466 c         2540 g         1447 t
ORIGIN      
        1 gggggcgtgg aagccgtagt ccatcaggcg tttggctacg tcttcggcca ttacggcgaa
       61 aaggccacgt tggcgctgaa gtacctggtt acgcagacca gcttcggcgc gggtaaggtt
      121 tgggacggct cgctggccac gctcgaaacc gtcgcgggta ttccgcggac cgacgcgttc
      181 gccacgctcc agcaggtgct cggcctggac gggcaacagg ccaccgcgct gctttgggat
      241 acgtacaacc cgcagtacat ctggtttccg ttcgccgcta tcggagtcgt cgccgcgatc
      301 gcgctggcga ttttcgggca actggcgaaa cgctggcgtg acatgaacgc ctagcgggtg
      361 taccgaaagc ccgggcggcc cgatcgccac gatccagcgg cagcccccta ggtacggggg
      421 gatgggcgat cgggccacac gacgcccttg acactgctac cgcaggtcgt aaaatcgccg
      481 ttctttgccc cttgagcggg gccttggccc gcttgtatcg ggaagcgtta tgaagaccgc
      541 caataagtgt ttcctcgcca aacccggcga ggttgataag aaatggttcg tggttgacgc
      601 ccaggacaag gtgctgggcc gtctggccac gcgcatcgcg accgttctca tgggcaagca
      661 caagccgacc tatacgcccc acgtggatac cggcgactat gtgattgtcc tcaacgccgg
      721 gaaggtccgc gtctccggcc gcaagcgcca gaccgaggtg taccagcgat acacccgtta
      781 ccccagcggg cgccgggtgg tcagcatgga gcaaatgctg gcccgtcacc cggagcgcgt
      841 gctgcgtgag gccgttcgcc gcatgctgcc gaaatccaag ctcggcgtgc acatgctcga
      901 caagctcaag ctgcacgtcg gctcgactca cccgcaccag gcccagaaac ccgaaccaat
      961 gccgttatag acggttgaac ttgaggaacg cacgtgagtg atatacccct tccgcccgat
     1021 caactgacct cccagccggc gactccggta actccggacg agccgaagcc ggccgaaggc
     1081 gtgctgcccg gcgcgccgac ggatgccgcc cctgctgccg ctccggttga aacgccccag
     1141 cccaccccgc cgcccggcgg gttctaccgt ggcaccggtc ggcggaaatc atcggtcgca
     1201 cgtgtgcggc tggtcgccgg cagcggcaag atcgtgatca acaaacgcga gctggagaac
     1261 tatttcaccg agccgcagga ccgggcggcc gtcatagccc cgctgcaggc cagtggcaaa
     1321 ctgggaatgt gggacgtgct ggtacacgtc cacggcggcg ggcataccgg gcaggcgggc
     1381 gccgtcaagc tgggtgttgc gcgtgcgctg gtagcggccg atgcatcggt cgagcccacg
     1441 ctgcgtgacg gtcaatacct gacgcgcgat tcgcggcgcg ttgaacgcaa gaagtacggg
     1501 cgccgcaagg cccgtcgtcg cttccagttc tcgaagcgtt aagccggaca tccgagcccg
     1561 ggcggaagcg aggaagacag ctattcgcga gcctcgccgt gcaagcggcg aggctcttct
     1621 tatgcgcggg agaggctgcg acggcggccg cactcggcca gcgagtgata aagtgataga
     1681 gtgataaagt gataaggtga taaagcgatt ccgcagatcg tcctttatca ccttatcact
     1741 ttatcaccct atcacctcat ggccgtcacg ggcagcgtga atgagcaaaa agcggcggcc
     1801 ggtccgctgt ggaccggccg ccgcttctca ggggcaggaa ttaccgttaa cgaaggcccg
     1861 cactgcttga agcttcggct ttgagccgtg gcaccccgcg ggcgcgtggg tcttagttct
     1921 tctgaacagg ctcacctctg tgcatcgggc attcggcctt cttctcggcg ggcttgtcac
     1981 atggttttgg acacggcttg tcgcacttgt cgggattgtg tttgcacttg gcggggcatg
     2041 gcttgcactt gtcggcgcag ggctcacact tacagcgctt gagataagcc gtccacgcct
     2101 tctcgcccgt cgggcacacc ttcatcttgc cgaaaccgag gagcccgact tccgcgatat
     2161 acgtccgcgc ggggccgccc tcaggcgtga atgtcagagt atcggtgtct ttgtcatagc
     2221 aatacgttcc gcacacctcg gccggcttcg cgtcttcgca ggccttgagg aggaacgtgt
     2281 tgtcgtctct cagcaccagg cacttcacgt cgaagttctg gcgttctgat tccggctcaa
     2341 tgcagtcgag agtccacttc cctgtgatgt cgccgcatcc tgtcaggaac aggacgcatg
     2401 tgaaaaaacc tgccattgcg aatcgtttca tgtcttacct ccacaaagtt cgtttcaaac
     2461 cgcacacgag cacgcattat ccggcgacgc tccggggatg caagctcgtt caggcatgat
     2521 cgccgatcga gccgacttgc ctttagtcga agacgggtga ctaaaaaccg tcagcactta
     2581 ggtatggtgt tcgccaaaac ggcatccaga agcgccgtgc agccgcaaat tgcgtcgcac
     2641 actggaatcc gcagctcctg cgccaacagg cgagtttcct cgcggaccaa tttcggatca
     2701 cagcccagcg gattcagcgc cacgccgaca acgcgcgatg gatgcagcaa tgacgccgcg
     2761 ctttcgtaga tggcccgcag ttcagccagt ctcggaatcc gcatgtgtgg cgcggcgctg
     2821 tagtgcgtgc ggcgggcatg gtgtacgagg atcatggcat ccgggcaggc gccatgaagc
     2881 aaggcgagag tgacggccga gaatcccgga tgagcgatgc tgccctgtcc ctcaatgatg
     2941 caaatgtctg aatcggcagc gctcagcacc agctcctcga ctgcgcccgg tgcgaagtcc
     3001 gaaacaactg catcaatggc aacgccgcgg cccgcgatca tcgtaccggt ttgcccggtc
     3061 gcgaggaatt cggcccgcag gccgcgctgc cgtgcggcgg ccgtcagttc cagaccggca
     3121 accattttcc cgacattccc gtccgtgccg acggtcaaaa cgcggcggca gcgcgtcgat
     3181 agagcgcgcg cggtcgcgat tgtccgctcg gccgggggac ggcgcaggtc gaaaatgcgg
     3241 gctcggtttt gctgtgccaa ggccgccaac tccgcatcgt ccgccaggaa ttggtgcaag
     3301 ccgctgacga cgtccatccc ggcggccaac gcatgggcga tgtgctgtcg catgggaccg
     3361 ggcaacgcgc cgcccggcgg agcaatacct acgaagagag cgtctggcgc cagggtgcgg
     3421 gcagcggcga tgtccgccac gatcggcacc gggggcgacc agggaaagac ggttcgcacg
     3481 tcggttccag ccgcgtgcga atcaagcacg cccacgacgt ccggcgcgcg gtagcgcagc
     3541 agggcggcgg cggttttgga cgtgaacgcc ccaagctggc cttcggtcag caggaggata
     3601 cggcgatacg gtggcagcac gtgcatgtta gttcttgcgc gtcgcgcttc ggcaaatgcg
     3661 gccggcgggc cgtgccgcga gtttggccgt agtatgctga gacatcaacg gcgtgccaag
     3721 ggtaagattc gctgaccatg cgaaagcaga gagcgctggt cgcgctggcg attgtatttc
     3781 tgccgggcgt catgctggct ccgctctggc ggcttggcgg cctcggggcg ggcgaggatg
     3841 acctgcttta ttactttccg tcgcggagct ttttccacga cacggtgcgc gcgggacaat
     3901 ggccctggat caatccgtgg accgggctgg accggccctt cgcggccgat ccccaatcgg
     3961 cgctgtggta ccccggcacg tggctgttcg cgctgctgcc gccgctgccc gcctacgcgg
     4021 tgagcctgtg ggcgcactac agcctggcga tctggggtat gtaccgcttg ctgcgtgcgg
     4081 ggcggctggc gcgaccggcg gcgctgtttg gcgggcttct gttcgcgttt tccggcttca
     4141 tgctggccca ccgggcacac ttctcgttgc agcatgccgc agcctgggca ccctgggtgt
     4201 tctggcggat tcagcgctat gcgcagaacg ggggcgggtt tcggctcact acggccagca
     4261 tcgtggcagc gcttcaactc tacgccgggc acgtgcagat tgccgcgatc accgccttag
     4321 ggagcctggt gttcatcgtt gcggccggct gggcagcagc gcccaagttc accgttaagc
     4381 tgcgcgccgc ggtcccggcg tttggtcgat ggcttggaat ctgggtgctt gcgggcgggc
     4441 tgttcgcggt ccaactcgtg ccgacggtga tgtggccgga gcagtgtacg cgcgttgacc
     4501 gcacgtatcg ggattttgtc gagaatagct ggtacccgtg gtcggcgatc gggtgggtgg
     4561 cgccgatgtt cttcgggcag cgcacgccca acttcttcga tcagccgtat tgggggccgt
     4621 cgcaccaatg cgagcaattc gcgtacgccg gaatcgtgcc gctcatattg gcggcggtcg
     4681 ctttgcgggt gggctggcgc ggggatccgc aacgccgacc gtggatggtc ctgctcatct
     4741 ttggcgtgct ggtggcgcta ggccagttcg gcccattgtg tccgctgctc tatcagattc
     4801 cgggcagcag cctgtttcgc gtgcctgctc gggcactgct gctgttcaac atgtccatcg
     4861 ccgctttggc cgccatcagc ttgcacgacc tcggcgccgc gcacaatccg cggcgcgtcc
     4921 gcctgcgcgc tgcattgcag gcctggagcg cccggccgca catcaagggc gccgcgttag
     4981 tgggcatgcc cctcatcgtc ggggccgcgg ttataccgtt cttgccgtca gactggcgtg
     5041 cggcggcact gcacgctctg cgcccgtgga gtccgagcgt tttcgtaccg ctggcggtcg
     5101 ctatggcgag cttcgcttcc ctgggagtgg cggcccgccg ttggcatcga ccgcgctggc
     5161 tgtggttgcc ggtcattgta acgacgcttg atttggcggt tatcggctgg accattgaca
     5221 ttcctttggg caagaacggg cccgcagacc ttctgccgca gagcgaagcg ccctgggtgg
     5281 aacgcgtgcg cgactcgggc cggcggcttt gggtggttgc acatacggcc gacatctatg
     5341 gcgagccgct taacattctg gccgccaacg ttaacagcct cgtgggcatt caatccttga
     5401 cggactatgg gccgttgcag ccgcgttcgc tgcagccgcg cttcgcgttc cggccctggg
     5461 gtgagagtga ccaggtccgg gccttgctcc aagatccgcg gtggatgcgc acttacaacg
     5521 tcggatggat tcttacgcgc gacccagcgg atgaacctgc cggctgcact ctcgaggagg
     5581 tggacgctgc tggttggcgt ctgtaccgca acccgtcggc cgccggaatg gccgttctgg
     5641 aagacgccgc ggcgcccgtc gcgattagca gccaagtgca ttcgaatcag cggttcgaag
     5701 tagtagtaga ctcctggctg gcgggcgagc gccacaggcc gggccgtacg gaggcggggc
     5761 agatgcagga aaagcggctg atcgtttcgc ggctggcgca gcccggctgg caggcgcgca
     5821 tagctaaccg gcaggtcgcg atcgagccgc gcgacggtct gatgtcgctg cgcgtgccgg
     5881 ccggcgaata cgtgaaaatc gacttctcgt actccccgcc gggtctgcgc gaaggcctac
     5941 tggtgagcgg cgcgaccgtg atagtgattc tggtgctggc ggcgcccggc tcgcgccgca
     6001 cacgtcaagg ccaggaggcg accgcgtcaa gcgccgtctg cacagcctga gggcgtttcc
     6061 gcgggccagc gcagagctac agtgcagccc cgggtctcgt tggcacgaac cgcaatgcgg
     6121 gcgccgcact tgtggacgat gatccggcaa acattcaggc ccagaccaat gccctgccac
     6181 tcgcggccgc gacactcagt gccggccaga aacggattta gaacgcggtc gatttgttcc
     6241 tgcgtcagcc cagacccgcc gtcgcagaac tcgagaacca cgtccgttcc attgcgccag
     6301 ccggtgacct tcaaactgcc cggtgtgtgc accatagcgt tacgcgcatt cagtatgaga
     6361 ttcaacacta cctggatcag caggtcgcgc cgggcacgca cgtgcaaatc cgggggaaca
     6421 tccacggtca gggcgatgcc atccttctcg agtggccgca caacggtggc caacgcttca
     6481 ttcaccgcct ctagaacgtt gcaggattcg agctggcatt cgcggttgtg ggcccaatcc
     6541 atcattcgct gggtaatact gacggcccgc tcgacttgaa ccttggtgcg ctccaatgcc
     6601 ttgcgcatct cagccggaga gccgctggca agggcgaact ccgcccgcgc cagcagagga
     6661 gtcatcaggt tgttgacttc atgtgcaata cccgccgcta tgttcccaag cgctaccaga
     6721 tgctggcagg cgatcagttc ctgctccagg ctcgcaatag ttcgctgtgc cgagtcggat
     6781 gagtcaggcg agactggacc ggctgttgcg ccggccggag ttgtaggctt tgggctggaa
     6841 ggcgacgcca tatctagcct ccccgcggtc agcaatactg ccgcgccgct gcaatcggct
     6901 ccacgcccaa gtaccccgat aataacgtgg ttccaggccg tgtcaatgac gggaatgcgt
     6961 ccattgttgc cgtaacgtcg ccgcactgtt ggcgccgcag gagcagcggc tgcaacggcc
     7021 cgcaaagtac cgagacttac gcccgtcgcg ccgccaatcg ccgcagccgg cgcaaattca
     7081 gccaatccca gtcccgccca gcgccatcct gccccttcgg agtctcgatg atcattggta
     7141 tttcgcagaa gcgcgaatcc gatagcagcg tgcggaaccc cgacgctcca atacggccgc
     7201 ggccaatatg ctcgtgccgg tccacccgag aaccgagagg cgcccggctg tcattaagat
     7261 gccagcaatg aatgcgttcg agcccgaacg agcgctcggc cgacgcgacc atttcctgat
     7321 atccctgcgg ccggcgaatg tcgtaaccgg cggcaaagag gtggcaggtg tccaagcaga
     7381 cgccggcccg cgaccgtcgc agacgcccga tgatctcgcc cagctcttcg agccgccagc
     7441 cgagggccgt tccctgcccg gcggtgtttt cgagcaatgg cgtgacctgc ggcgcgaccc
     7501 cccgtttggc caagtcgtcg agcgcggccg cgactcgcgc gatcgccgag cggcggtcgc
     7561 cgtcggttgc cgcgcctgga tgcaccacga gatacggtat atgaagggtt tggcagcgtt
     7621 gaagttcttg ttcaaaggct gccaggctgc gcgtccggac gattttgtcg gccgatgcca
     7681 ggttgatgag gtacgtcgcg tgggcgatga ccgggccgaa gccgggagta tccaaaaggg
     7741 cgttccagca gcgcaggtca tccgcgcgta gtggggccgc ccgccattg
//
LOCUS       DAOSYS010000174         7749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8041_length_7749_cov_4.354822, whole genome shotgun sequence.
ACCESSION   DAOSYS010000174 DAOSYS010000000
VERSION     DAOSYS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_7749_cov_4.354822"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PLU99_11015"
     CDS             <1..920
                     /locus_tag="PLU99_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOO17626.1"
                     /translation="ELDNAYELADPFRPFTLGGIWTARETWAPVYHVRTGLRDLDTGL
                     GGGLRVGGVHLFVGKSGRAKSTTVAQIALNAARAGVPACIVSLEMPRRDMGHLIAANL
                     ADVPRAALASGNIRETVKDKLRGVLREYGKLPLTILDDSFWSGPLTRSKLAEIVADGC
                     RRFGWRLICVDYLGLLANEPTDASDYAADLENSAALKRLARVQDVALVAVAALRKYGR
                     ANSDAPASLDDVLGAGRICYDAVNVFDVDCQQDSPRPGERPVGTVRLRVLKTRYAGLG
                     SAGKPLEFRWFPSTGRIEDPNPQWGLPNE"
     gene            1398..1985
                     /locus_tag="PLU99_11020"
     CDS             1398..1985
                     /locus_tag="PLU99_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17627.1"
                     /translation="MKPPPLATAADFRATLATVEGGDCRAIHVYRLGSTILKFYQSLT
                     VEDFERVARKLVEIARNKKMAARVRLRAIQATLAPLAAGVKLLAKLQARTSPAVLHLE
                     ACLGAFYGALEADMPTLINTLRDMTKVSNTTGIRASAALLKVTFEMVDALVTIRPRAE
                     EAPVRDPARQAKIEADLAEMEQRILAKNAGQEHQS"
     gene            2249..2461
                     /locus_tag="PLU99_11025"
     CDS             2249..2461
                     /locus_tag="PLU99_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO17628.1"
                     /translation="MKASETLRKAIERADISRYAIAQRTGVSESMLCRFVQGRPITTD
                     TFDKLVDALGLELVPKTRGRTRKEGK"
     gene            complement(3719..3792)
                     /locus_tag="PLU99_11030"
     tRNA            complement(3719..3792)
                     /locus_tag="PLU99_11030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3756..3758),aa:Val,seq:cac)
     gene            4128..6416
                     /locus_tag="PLU99_11035"
     CDS             4128..6416
                     /locus_tag="PLU99_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17629.1"
                     /translation="MNSRKTAHFRPACTCHLLLSALWLALCAGCAPANLSPTAYDVSV
                     RLDPATHRLNGETLITLRPDPPSGWPRQWVTLDFALNRALRIEKITAEDAAVVSHATI
                     EPPPEPEDPAAASIATRGAATQPVTASEQPLCLIHRFVLGSLGASPRLKLDYSGELVQ
                     DVQAGERRGQIHNRLVAAHIGPEGIYLDERGGWYPFSYRQSDRPVGELAAYRLTVEPV
                     PGMELVAGARFDHEASSANGKLVWNSTYPLEGLVLVGGPHRIKTRDADGIRLRLHYSL
                     PEDEQSRDVIEKNTDLFLAAAADYLARYQPLIGPFPFRDYAIVENFFSSGFAFPEFTL
                     LNKTLFQMGRRALGHGFLDHELLHCWWGNGIYVDPADGNWCEALTSYAANYYGYVLDG
                     DDQGARKQRRNCCMIVSGIKPEQDKPLGSFDRPNGPGREVGYNKGTMVFHMLATRVGQ
                     ENFWNAMRRLTRDYMGRYASWSTLQEVCEQESGLELDRFIQDWVRTAGAPRIELTSAV
                     WLPAESALDVTIAQHDTNFELNVPLRLVHDDGSTRDEIIPLDGPAATVRLSSDRPVRT
                     VVLDPDYQILRKVRTEEMIPNASLVRSARDLLIVTPPEPISPFYQIVIEDCTGPAGSK
                     QITHRTAADVTAADLAGKSVLLLGDAVRAEPVQALLARTVCPIQWETNGFRIDDTLYN
                     SPSHAVLCTVHHPDSPEQAVMLYYGNSETALGRADLLGFYRDSLVVFETSSRQVRDET
                     IYEGRPIARRDFETLQVIEVSR"
     gene            6416..>7749
                     /locus_tag="PLU99_11040"
     CDS             6416..>7749
                     /locus_tag="PLU99_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17630.1"
                     /translation="MPRRTKVRQRLPGAQGSALPPQGKVSRATTSRAARRSLVLRIAH
                     SPGGRAVLLGAAAGLVYLFVAGDLRFHFVQSPHPHLLLLADAFLHGQSHVRPQQLAAV
                     QQRDEAAADQALAALAQRRNIAISELQRRQFLEQWHRGRSLLDWSVVDGKYYGYWGPL
                     APALLTPVVALFGPGVSDRLIDALFGALNVALFYVLLRRADRAGWCATTEPARIALTV
                     LLAFGTVHFYSACIGSVWFSVQIITLTALLVACILSCAPQLTWRAALLSGAAFGAAIL
                     GRNIVVLIAPYFLTLFWFQTSGPRRARLAAWLRNCASFALPCIVAILVQGAYNQARFG
                     SPFDSGQKALMQTAGAAELAPDYYRYGQFHPHFLATNAKFYFWNWELSRDGHGRLSFD
                     GRGNSMFIVTPPLLYLFLAWRRRNRLALALAAGVVPFMAGLLLFRTTGAYQFG"
BASE COUNT         1406 a         2501 c         2497 g         1345 t
ORIGIN      
        1 cggagctcga caacgcgtat gagctggcgg acccgttccg cccgttcacg ctgggcggca
       61 tttggacggc tcgcgaaacg tgggcgccgg tttatcacgt gcggacgggg cttcgggacc
      121 tggacaccgg gctcggcggc gggctgcgcg tcggcggcgt tcacctgttc gtcggcaaga
      181 gcggccgggc gaagtctaca acggtcgcac agattgcctt gaacgccgcg cgggcgggcg
      241 tgccggcttg cattgtgagc ctggaaatgc cgcggcgcga catgggacac ctgattgcgg
      301 cgaatcttgc cgacgtgccg cgggccgcgt tggcaagcgg caacattcgg gaaacggtga
      361 aagacaagct ccggggcgtg ctccgggaat acggcaagct gccgctcaca attctggacg
      421 attcgttttg gtccgggccg ctcacgcgct ccaagctcgc cgaaattgtc gccgacggtt
      481 gccggcggtt cggctggcgg ctcatttgcg tggactattt ggggttgctg gcaaacgagc
      541 cgacggacgc gagcgactac gcggcggacc tggagaactc cgcggcgctc aagcggctgg
      601 cacgcgtcca ggacgtggcg cttgttgcgg tcgcggcgct gcgcaaatac ggccgcgcga
      661 actccgacgc gccggcttcc ctggatgacg tgctcggggc gggccgaatc tgttatgacg
      721 ctgtgaacgt attcgacgtt gactgtcagc aagattcgcc gcgcccgggc gagcggcccg
      781 tcgggactgt gcggctccgg gtgctgaaaa cgcgctatgc ggggctcggc tcggctggca
      841 agccgctcga attccgctgg ttcccgagca cggggcgcat cgaagacccg aacccacaat
      901 gggggctgcc caatgagtga caaacgtcag gcacaaccaa aaccgtggac gctggcatgc
      961 ttgtatgcgg aatgtcattt ggcacctacg gcatggctgg cattagtcga aatggctgaa
     1021 cggcggggct ccagtattgt aaccccaacg cgcgaggatc tccgggcggc aacgggggcg
     1081 cgaacgctga aactcgtttc agccgcactt accgcgttgt cgcgtgcccg ctggatcatt
     1141 cgcaaccacg ttcctctgtt cacgggcgcg cagcaaactg caacggcgtt gcgtatcaag
     1201 ctctgtttcc gtagagggcg aaaatcgacc catacggcga gcaacccagt agggcgaagt
     1261 ttcctgcctt ggcacgcact cagattctgg agagttagag caacatgaag cacacagacg
     1321 cagacggtcg tcctgaacgg cgagaactcg caaagcaaca tgcccgcgaa cacgggtacg
     1381 acggttcttg gccaccagtg aaaccgcccc cgctggcgac ggcggcggac ttccgcgcga
     1441 cgctggcgac ggtcgagggc ggtgactgcc gggcaatcca tgtgtatcga ttgggctcca
     1501 cgatcctgaa gttctaccag agcctcacgg tcgaggactt cgagcgtgtc gcgcggaagt
     1561 tggtcgagat cgcgcgcaac aagaagatgg cggcgcgggt ccggctcagg gcgattcagg
     1621 ccacgctcgc acccctggcg gccggcgtca aactcttggc gaagttgcag gcccgcactt
     1681 cgcctgccgt cttgcaccta gaagcgtgct tgggcgcgtt ctacggggcg ctcgaagcgg
     1741 acatgcccac gttgattaac acgttgcgcg acatgacgaa agtgagcaat acgacgggga
     1801 ttcgagcgtc ggcagcgctt ctgaaggtca ccttcgagat ggtggacgcg ctggtgacga
     1861 ttcggccgcg ggctgaagaa gcgccagtga gagacccggc ccgtcaggcg aagatcgagg
     1921 cggaccttgc ggaaatggag cagcggatac tggcgaagaa cgcggggcag gagcaccaaa
     1981 gttgatagca gcagtgattg aacagcaggc gccgaaggtg gcgcagttac tcttgaagcc
     2041 ggcggaagcg gccaaggcgc tcgggatcgg ctcgcgcacg ctttggggcc tgacgaaggc
     2101 tggcgagata gcttgcgtcc gggtcgggcg ctctgtgcgc tacgcgacgg cggaccttca
     2161 ggcgtgggtc gaaaaaaagc gcgcgggaaa ttgagcgcgc gatcacccgt tgcttgcttc
     2221 tgcggaagta tgaagtatac ttaagggtat gaaggccagc gaaacattgc ggaaggcgat
     2281 tgagcgggcg gacataagcc gttacgcgat cgcgcagcgc acgggcgttt ccgagtcgat
     2341 gctctgccgc tttgtgcagg ggcggccgat taccacggac acattcgata agctcgtgga
     2401 cgcgctcggt ctggagctgg tcccgaaaac gcgcggccgg acgcgaaagg aagggaagta
     2461 agtatggcga gtatcagtcg agaaccgaac ggacgccgta ccgtgcagtt tgtcggcgcg
     2521 gacggcaagc gccgcagtgt ccggttgggg gagtgcaatc agcggcaagc cgaaatcgtg
     2581 cgcctgcacg tcgagcggct ggccgcggcc aagcgcaccg gcggcaacgt cccggaggat
     2641 acggcgcgat ggctcgatca gattggcgac attctggccg aacgactcgc taaggcggac
     2701 ctgatcccgc cgcgggcgtc ggcgaccctg ggggcgttca tccgccagta cattgacggg
     2761 cgaacggatg tgaagcattc cacccgaatc accctggagc gggcgcgggc caagcttgtg
     2821 gaacacttcg gggagagccg ggatttgcgg acgatcaccg aaggcgaggc ggacgaattc
     2881 gcgctctggc tcgaacgtga caacggcggc aagctggccg cggcgacggc gcgtaaaacg
     2941 gtgagcatcg ccaagcagat gttccgggcg gcacgccggc ggaagttgat tcgcgagaac
     3001 ccttttcagg atttggccgg cgctgttccg gcaaaccgta gccgtgatta tttcgtgagc
     3061 cgtgcggacg cggaccaggt tctggccgcg tgtcccgaca tacattggcg gaccctgttc
     3121 gccctggcgc gcttcggtgg cctgcgcacg ccgagcgaat cattcgggtt gaagtggaca
     3181 gatgtaaact gggaaacggg gcgctttacg gttcgcgcgc caaagactga acggcacgcg
     3241 ggccatcaga cgcggattgt tccgctattc ccggaactcc gggcggtgct gatggaagcg
     3301 tttgaagccg cggagcccgg cgccgtgtac gtgctgccct ggcgggatac gacgatcaat
     3361 ctgcgcaagg gcctgatgac gattatcagg cgcgcgggcc ttgagccgtg gccgaaactc
     3421 tatcagaacc tcagggcgtc acgagaaacg gaactgctgg caatgttccc gcaacactgc
     3481 gtactggcct ggatcgggca ttcccggcag gtgagcttgg cgaattacgc gcaggtgctg
     3541 gacactgatt ttgagcgcgc gatagccgac gccaaagcgg tgcagaatcc ggtgcagcac
     3601 gctgccgccg ggtcgcgcct ggactcgcaa cacgacgaac cggagctagt gaaaagcccg
     3661 gtttgcgaga gctggcgagg ggaggcggct gccggcgaga catttctaga aaatcaagtg
     3721 ggcgttacag gactcgaacc tgtgacctcg tccgtgtgaa ggacgcgctc taaccaactg
     3781 agctaaacgc ccgcgatccg cgcacgctct gacggccaag cgagcaaaac gcccgcgagc
     3841 cgccgacggc ggtttctttc cacaacttca ttttctgcca tctgccgcct gttcgtcaag
     3901 tgccgcccgc aaccctaagg aacgtgcgag acccgggctg cgcggcaccg ctgccttagc
     3961 tcaaagctga gggccacgag ctgatcgctc gcccgttgac gccgcggtct attgcagaga
     4021 catttgttgc cgcacgctcg cgggccgacg gttcgggtga ttcggatcgg accaatcccc
     4081 tcgccggcgc cgcttgcccg gccccgccag cggccaagga ggttgcggtg aactctcgaa
     4141 aaacggccca tttccggcct gcctgtacct gccacctgct cctgtccgcc ctctggctcg
     4201 ccctgtgcgc cggctgtgcc cccgccaacc tctcccctac cgcgtacgac gtctccgtcc
     4261 gcctcgaccc cgccacgcac cgcctcaacg gcgaaacgct catcactctg cggcccgacc
     4321 cgccctccgg ctggccccgc caatgggtga cgctcgactt cgccctcaac cgcgccctcc
     4381 gcatcgaaaa aatcacggcc gaggacgccg ccgtcgtcag ccacgcaacc atcgaaccgc
     4441 caccggagcc cgaagacccg gccgccgcct caatcgccac gcgcggcgca gccacccagc
     4501 cggtgaccgc ttcggagcag cccctgtgcc tcatccaccg cttcgttctc ggctcgctcg
     4561 gcgcctcccc gcgcctgaaa ctcgactact ccggtgaatt ggtccaggac gtgcaagccg
     4621 gcgagcgccg cggccagatt cacaatcgcc tcgtggccgc gcacatcggc cccgagggca
     4681 tctacctgga cgagcgcggc ggctggtacc cattctcgta ccgccagtcc gaccggcccg
     4741 tgggcgagtt agccgcttac cggctgaccg tcgagcccgt gccgggcatg gaattggtgg
     4801 ccggcgcccg cttcgatcac gaggcgtcca gcgccaacgg caaactcgtg tggaactcga
     4861 cctatccgct cgaggggctg gtgctggtcg gcgggccgca ccgcatcaag acgcgcgatg
     4921 cggacggcat tcgtcttcgg ctgcactatt cgctacccga ggacgagcaa tcgcgcgacg
     4981 tcatcgaaaa gaacacggac ctgttcctcg cggcggccgc ggattacctg gctcgttatc
     5041 agccgctgat cggccccttc ccattccgcg actacgcgat tgtggaaaac ttcttcagct
     5101 cgggcttcgc ctttcccgaa ttcacgctgc tgaacaaaac gctcttccag atgggtcgcc
     5161 gcgccctggg gcacggcttc ctggatcacg agctgctgca ctgctggtgg ggcaacggca
     5221 tttacgtcga tcccgccgac ggcaactggt gcgaggcgct taccagttac gccgcgaact
     5281 actacggcta cgtcctcgac ggcgacgacc aaggcgcacg aaaacagcgg cgcaactgct
     5341 gcatgatcgt cagcgggatc aagccggagc aggacaagcc gctgggtagc ttcgaccgcc
     5401 cgaacggccc gggccgcgaa gtgggctaca acaagggcac tatggtcttc cacatgctcg
     5461 ccacgcgagt cggccaggaa aacttctgga acgccatgcg gcgcctgacc cgcgattaca
     5521 tgggccgcta cgccagttgg agcacgctgc aagaagtctg cgagcaagag agcggcctgg
     5581 agctcgaccg cttcatacag gattgggtgc gcaccgccgg cgcgccgcgg atcgagctga
     5641 ccagcgcagt ctggctgccg gcggaaagcg cgctcgacgt cacaatcgcg cagcacgaca
     5701 ccaatttcga gctgaacgtg ccgctgcgtc tggtccatga cgacggcagc acgcgcgatg
     5761 aaatcatccc gctcgacggc cccgccgcga ccgtgcgcct cagctcagac cggcccgtcc
     5821 gcaccgtcgt gcttgatccg gattaccaga ttctgcggaa agtacgcacg gaggagatga
     5881 ttcccaacgc gagcctcgta cggtccgccc gcgacctcct gatcgtcacg ccccccgaac
     5941 ctatctcgcc cttctaccag attgtcattg aggattgcac cggcccggcc ggttcaaagc
     6001 agatcacgca ccggaccgcc gctgatgtca cggccgccga cttggccggc aagtcagtat
     6061 tgctgctggg cgacgccgtc cgggccgagc cggtgcaagc gctgctcgca cgcacagtct
     6121 gccccatcca atgggagaca aacggctttc gcatagacga cactctctac aattcgccca
     6181 gccatgccgt gctgtgtacc gtccaccatc cggattcacc cgaacaggcg gtaatgctct
     6241 attacggcaa cagcgaaacg gccctcggcc gcgccgacct gctgggcttc taccgcgaca
     6301 gcctggtggt tttcgaaacc tccagccggc aggtgcgcga cgagacaatc tacgagggac
     6361 ggccgatcgc gcggcgcgat ttcgagacgc tgcaagtgat agaagtgagt cgttagtgcc
     6421 gcgtcgcacc aaagtgcgcc aacgcctgcc gggagcgcag ggctcggctt tgccgccgca
     6481 aggcaaagtt tcgcgcgcca caacctcccg cgccgcccgg cgcagcctgg tactccgtat
     6541 cgcccattcg cccggcggac gcgccgtgtt gctgggagcg gcggccgggc tcgtttactt
     6601 attcgtagcc ggcgacctgc ggttccactt tgttcagtcg ccccatccac acctgctgct
     6661 gctggccgac gccttcctgc acggacaatc gcatgtgcgc ccgcagcaac tggccgccgt
     6721 gcaacagcgc gacgaggccg cggcggacca ggcgctggcc gctctggccc aacggcggaa
     6781 catcgcgatc tcagaacttc agcgccggca gttcctggaa cagtggcatc gcggacgcag
     6841 cctgctggat tggtccgttg tggatggcaa gtactatgga tactgggggc cgctggcgcc
     6901 ggcgctgctg acgccggtag tagcgttgtt cggccccggc gttagcgatc gcctgatcga
     6961 cgcactgttc ggcgccctga acgtcgcgct attctacgtg ctgttgcggc gcgcagaccg
     7021 cgcagggtgg tgtgcgacta ccgaaccggc ccgcatcgcc ctaaccgtcc tactggcgtt
     7081 cggcacggtt catttctatt ccgcttgcat cggctcagtc tggttcagtg tgcagattat
     7141 cacgcttacg gcgctgttgg tggcctgcat cctgtcgtgt gctccgcagt tgacgtggcg
     7201 cgccgcgctg ctcagtggcg ccgcgttcgg agcggcgatc ctcggacgca atattgttgt
     7261 tctcatagcc ccctactttc taacgctgtt ctggtttcag accagcggcc cgcgccgggc
     7321 gcgcttggca gcatggctga gaaactgcgc atcatttgca ttgccctgta ttgtggcaat
     7381 tcttgtgcaa ggcgcctaca accaggcccg cttcggaagc ccgttcgaca gcggccaaaa
     7441 agcgctcatg cagactgccg gtgcggccga gctggccccg gattactacc gctatggaca
     7501 attccacccg cacttcctgg cgaccaacgc caagttctat ttctggaact gggaactgag
     7561 ccgggacggg cacggccggc tcagtttcga cggccgcggc aacagcatgt tcatcgtgac
     7621 ccctcccctg ctctatctgt tcctggcttg gcgccgccgc aatagactcg ccttggcact
     7681 ggcggcgggc gtcgtgcctt tcatggccgg tttgctgctg ttccgaacca ccggcgccta
     7741 ccagttcgg
//
LOCUS       DAOSYS010000175         7731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8063_length_7731_cov_4.877931, whole genome shotgun sequence.
ACCESSION   DAOSYS010000175 DAOSYS010000000
VERSION     DAOSYS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8063_length_7731_cov_4.877931"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..431
                     /locus_tag="PLU99_11045"
     CDS             114..431
                     /locus_tag="PLU99_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17631.1"
                     /translation="MYARRKIAGLMILVGVPLLAAVLWLASGRETFTKSGRAVEVAVR
                     DPLFGDMVTETRLERGPIFGYYIGLDLLLLALLLAVVVGAVWWLLARHRRRLDTSAKE
                     ATP"
     gene            428..1186
                     /locus_tag="PLU99_11050"
     CDS             428..1186
                     /locus_tag="PLU99_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOO17632.1"
                     /translation="MRLSVFALRLIALTAILTTSALPQVFATGLDVNASAPKTFYVNP
                     RAGNSQVTVFSRSTLEDFTTVCNRVSGEWQVDPKNLETFRGRFSLRVEDMHTGIELRD
                     RDMRGAEWFDAARYPEVVIEISRAENVRPASANSASMNLVGTCTMHGQTNPVSIPATL
                     AYMVESPQTMKLVKGDVIRIRAEFTVKLSDYGITGPRGSEIIGVKVSDEQEVKVTIFG
                     ATEKPPEELAVDTQPAAGGGAVPPPPPPPPPPPN"
     gene            1224..1973
                     /locus_tag="PLU99_11055"
     CDS             1224..1973
                     /locus_tag="PLU99_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOO17633.1"
                     /translation="MCNLFPALRVMSLLTVGLVAWGAPHALATALDVKASGEKAFYLN
                     PRAANSQITIFSRAPLEDFTTVSNRVSGEWRVDPKNLESTRGYFAMRIEDLRTGLDVR
                     DRDLRGPDWFDAARFPEIKIEISRVTGVKKTGPNTASMTLVGVCSMHGHTNPVSIPAT
                     LAYLDESPLTMKLGGGDVIRIRAEFTLRLSDFGVTGPPGTEMIGFRVANEQEVKVALV
                     GVAGKPPEEVEVHQPRISGAAPAPAPLPPPR"
     gene            1993..3384
                     /locus_tag="PLU99_11060"
     CDS             1993..3384
                     /locus_tag="PLU99_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO17634.1"
                     /translation="MKTPWLPATLVLCTCLYGCCGQRQTVDCILPLPPAPLRPADHLY
                     TLDELVQLAILRNPGLDAARYSAEAVEGLVDQVKSLWLPSLRYDFAATTYNNDFSYPA
                     RPFGIATVNVPLTGAYNIFNNIALGQIVATGGKRTSGLKQVKMLAELARLDVLRKQDF
                     ITFGVATLYHLVCLSNDVDVVLEDTLRRMRVYRQVAEELTARGSLRSNRLNNLQADLA
                     ITALEQLQIAVRGGRQQAYAALKQLVGLDPSEPMLLASASLPPAVTPSDLLSAAEAVA
                     TGFLRRPETREVDLFAKIFAEQVRFAKAAWAPNVAFVLDGINVTGDGNTILDALHGVI
                     VGVIVDLPLYQPARRGRLRQALNLEQASLAIQREVEQIITLDIQTTVIHAQQALAIWL
                     HSARARQIAEEHFESAREAYSRELIMAPTLVVALGVDAAAKIANLTATFNYHVARANL
                     RRATAERSIPYGY"
     gene            3374..4900
                     /locus_tag="PLU99_11065"
     CDS             3374..4900
                     /locus_tag="PLU99_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOO17635.1"
                     /translation="MAIEPNHKSQPQAHEPEAHEPIPKAGRYAQSRKRGHAPTSTLTI
                     SACILLLSISQTTLAQSPTQPAALSPASQPAETQPTTAPTQTQPTTSPTQTQPTSAPS
                     TAPSTAPALQPVQLYDTTLARPICPRDWLEEHALERGPDLRLVDRLMDERTEPPPFCR
                     EPLPLYPPDARIAPDVPMLPDTDPFAPPTPLAHKEPTMHVGVARSTFHTREPAEVLAA
                     AAPFIDLTQRDVAVRGAAVITETAGEIFYGLLQQRIQMQISNVFDYLLVQSWFANVPD
                     NGTILLCWAAPAHTYPTELGRDLPGVPGTSIILVVDKNSPYRTPADLRNTRLALPHNY
                     VNAPGAFLTRTLEDLEVPPEQQFFSKVTLRRFSKDAVIDVLKGHADVACVDEGTLAAL
                     ADFYGINVRIRPVAVSPRYNLDVLFTSKNNLEDYRTEIELTQRQLTTLAKDPEGQEVL
                     FFFDESGWFNYRRGDLAVPREHFGDFVRFLTQTPVDLKLMLAPNPPVDRQTYTIIGDE
                     "
     gene            complement(5240..7333)
                     /gene="fusA"
                     /locus_tag="PLU99_11070"
     CDS             complement(5240..7333)
                     /gene="fusA"
                     /locus_tag="PLU99_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOO17636.1"
                     /translation="MTSPAQNRIQRIRNIGVSAHVDSGKTTLSERILFYSGRIHKMGE
                     VKGDGATMDHMDLERERGITITAAATTVDWLGHQVNLIDTPGHVDFTVEVERSLRVLD
                     GAILVLCGVGGVQSQSLTVDRQMKRYGVPRLIFINKLDRVGADPARVIAAVEEKLGLE
                     AAPLQLPIGLGEAFRGVVDLIEMQAVYFEGPCGEHVRREAIPEDLRDAAERARRALLD
                     TLSLYDDDLMEMLLEERQPERAYLESVIRRLTIARDIVPVLMGSAYRNKGVQPLMDAV
                     ARYLPSPLDRMYYAKDTANEGAEVAMTADPDAPAVAMAFKLVDEAYGQLTFTRVYQGT
                     LRRTDVLVNSRTRKTLRIGRMVRVHANEREDVDQAGAGDIVALLGVDCATGDTFCSEE
                     LSYSLENIHAPAPVISLAVAAANSRDRDKLAKALGRFAREDPTFHVRTDAETGETIMA
                     GVGELQLDVYCERIRREYGVVLITGAPKVSYRETPTRPAAFNYRHRKQTGGAGQYGHV
                     IGRLEPLPEGAEKVYEFENRVFGGRIPTEFIPSCDKGFQAARMKGPLAGYEVVRVKVV
                     LEDGSAHSVDSSDVAFQIAAREAFKAAYLESAPAILEPIMRCEVEVPGEFQGAVVADL
                     SARRALILGTEARAEATLIAAEAPLANMFGYATDLRSLTQGRGTFAMEFACYRVAPRA
                     VQEEVLAKARAGVPR"
BASE COUNT         1461 a         2722 c         2209 g         1339 t
ORIGIN      
        1 tccgctcatc cccgagcaat gccgtctaga atcgtcgtcg gcggccggag gacaacaccg
       61 gctcttgggt ggcgctccgc actcgccccg ctgcgccgga gaaagccaga tgtatgtacg
      121 cccgcagaaa aatcgcgggc ctcatgatcc tggtgggcgt cccccttctg gcagccgtgc
      181 tgtggctcgc cagcggccgc gaaacgttca ccaagtccgg ccgcgcggtg gaggtcgctg
      241 tgcgcgaccc gctgttcggc gatatggtga ccgaaacgcg cctggaacgc ggcccaatct
      301 tcggctatta cattggtctg gacctgctgt tgctggcgct gctgctggcc gtggtcgtgg
      361 gcgccgtctg gtggttgctc gcgcggcacc ggcgacgttt ggacacttct gccaaggaag
      421 cgacgccatg agactctcag tatttgcact gcggttgatc gctttgactg cgatcctcac
      481 cacatcagct ttgccacagg tgttcgccac gggactggac gtaaacgctt ccgcccccaa
      541 aaccttctac gttaacccgc gcgccggcaa tagccaggtc acagtcttca gtaggtccac
      601 cctcgaggat ttcaccactg tctgcaatcg cgtcagtggc gaatggcaag tggacccgaa
      661 aaacctcgaa accttccgtg ggcggttttc gctgcgcgtg gaagacatgc acaccggcat
      721 cgagctgcgc gaccgcgaca tgcgcggtgc ggagtggttc gacgccgcgc gttatccgga
      781 agtagttata gaaatcagca gggccgaaaa cgtgcgaccc gccagcgcca acagcgccag
      841 tatgaatctc gttggcactt gcacgatgca cggccaaacc aatccggttt ccattcccgc
      901 tacactggcc tatatggtag agtcgcctca gacgatgaaa ctcgtcaaag gcgacgtgat
      961 tcgcattcgc gccgagttca cggtgaaact gtcagactac ggaatcaccg ggccgcgcgg
     1021 tagcgaaatc atcggcgtca aagtatccga cgaacaggag gtaaaggtca ccatattcgg
     1081 cgccaccgaa aaaccgcctg aggaactggc cgtggatacg cagccggccg ccggcggagg
     1141 cgctgttcca ccgccgccgc caccaccgcc accgccaccc aattagcgaa gatcagcagg
     1201 tgacaaggat tcggccagct tgcatgtgta acctttttcc ggctctgcgc gtcatgagtc
     1261 tactaacggt tggactcgtg gcatggggcg cgccgcacgc gctggcgaca gccctggacg
     1321 taaaggcctc gggcgagaaa gcgttctacc tcaatccccg tgccgccaac agccagatta
     1381 cgatcttcag ccgggcgccc ctcgaggact tcactaccgt gtccaatcgt gtcagcggcg
     1441 agtggcgcgt cgatccgaag aacctcgaaa gcactcgcgg ctacttcgca atgcgcatag
     1501 aggacttgcg cactggcctg gacgtccggg atcgcgactt gcgcggcccg gactggttcg
     1561 acgccgcgcg ctttcccgaa ataaagatag agatcagcag ggtaacgggg gtcaagaaaa
     1621 ccggccccaa cactgccagc atgactctgg taggcgtctg ttcaatgcat gggcatacga
     1681 accccgtgtc gatcccggct accctggcgt acctcgacga gtcgccgtta acaatgaaac
     1741 tgggcggagg cgacgtaatt cgcatccgcg ccgagttcac ccttcgattg tcggatttcg
     1801 gcgtcaccgg tccgcctggg acagagatga tcggattcag ggtggcgaac gaacaggaag
     1861 tcaaggttgc actcgtcggc gtcgccggaa agccgcccga agaagtcgaa gtgcatcaac
     1921 cgcgaatttc cggcgcagcg cctgcgcctg ctcctctgcc gccgcccagg taataaaaca
     1981 gcgaagacaa aagtgaagac accttggctg cccgcaacgc tagtgctctg tacgtgcctg
     2041 tacggctgct gtgggcaaag gcagacggtg gactgcatac tgccccttcc gcccgcgccc
     2101 cttcgccccg ccgaccatct ctacacgctg gacgaactgg tccaactggc gattctccgc
     2161 aatcccggcc tggacgccgc ccgctacagc gccgaggccg tggagggact ggtcgaccag
     2221 gtcaagtccc tctggctccc ctcgctgcgt tacgatttcg ccgccacgac ctacaacaac
     2281 gatttcagct atcccgcgcg cccgtttggt attgctacag tcaacgtccc gctcaccggc
     2341 gcatataaca tcttcaacaa tattgcactc ggccagattg tcgctaccgg cggcaagcgt
     2401 accagcggct tgaagcaggt caaaatgctg gccgagctgg cccgcctcga cgtgctccgc
     2461 aagcaggact tcattacttt tggcgtggcg accctgtacc acctggtctg cttgagcaat
     2521 gacgtggacg tcgtgctgga ggacacgctg cgccgcatgc gcgtctaccg ccaggtggcg
     2581 gaggagctca ccgcccgtgg ctccctccgt tccaacagac tgaacaacct gcaggccgat
     2641 ctggccatca ctgcgttgga gcaattgcag atcgccgtgc gcggcggccg ccagcaggcc
     2701 tacgcggcac tcaagcagct cgtgggcctc gacccgagcg agcccatgct gctggccagc
     2761 gcgtcgctgc cgcccgccgt aaccccaagc gatctgctca gcgccgcgga agcggtggcc
     2821 acggggttcc tgcgacgccc ggaaacccgc gaagtggacc tgtttgccaa gatatttgcc
     2881 gagcaagtga ggttcgctaa ggcggcctgg gctcccaacg tcgcgttcgt cctggacggg
     2941 atcaacgtta ccggcgatgg caataccatc cttgatgcgc tgcacggagt gatagtcgga
     3001 gtcatcgtgg atttgcccct gtaccagccc gcacgccgcg ggcgcttgcg ccaggcgctc
     3061 aacctcgagc aggcttcgct cgccatacaa cgcgaggtgg aacaaatcat taccttggac
     3121 atccagacca cggtgattca cgcccagcag gccctggcaa tatggctgca ctccgcccgg
     3181 gcccggcaaa tcgccgaaga gcacttcgaa tcagcccgcg aggcatacag ccgcgagctc
     3241 attatggccc caacgctggt cgtggctttg ggcgtggacg cggcagccaa aatcgccaac
     3301 ctgaccgcga ccttcaatta tcacgtcgcc cgtgccaacc tccggcgcgc caccgccgag
     3361 cggagcatcc catatggcta ttgagcccaa ccacaaatcc cagccccaag cgcacgagcc
     3421 tgaagcgcac gagcccattc ccaaagctgg gcgctatgcc caaagccgta agcgcgggca
     3481 tgcccccacc agtacgctca caatctccgc ctgcatactc ctgctctcca tcagtcagac
     3541 cacgctagcg cagtccccca cccaacccgc cgcgctatca cccgcctctc aaccggccga
     3601 aactcagccc accaccgcac ccacacaaac ccagcccacg accagcccga cccaaacgca
     3661 acccacgagc gccccgtcca ccgcgccatc gaccgccccg gccctgcagc cggtccagct
     3721 atacgacacc acgctcgccc gcccgatctg cccacgcgat tggctggaag aacacgcctt
     3781 ggagcgcggc ccggacttgc gcctggtcga ccgcctcatg gacgagcgca ccgagcctcc
     3841 gcccttctgc cgcgagccac tgccgctcta cccgcctgac gcgcgcatcg cccccgatgt
     3901 gccgatgctc ccggataccg accccttcgc cccgcccacg ccgctggccc acaaggagcc
     3961 gaccatgcac gtgggtgtcg cccgttcgac gttccacacc cgggaaccgg cggaggtcct
     4021 ggcggccgct gcaccgttca tcgacctcac gcagcgcgac gtcgcagtcc gcggggccgc
     4081 ggtcatcacc gaaacggccg gcgaaatctt ctatggtctg ctccagcagc ggatccaaat
     4141 gcagatcagc aacgtgttcg attacctgct cgtgcagagc tggttcgcca acgtgcctga
     4201 caacggcaca atcctgctct gctgggctgc ccccgcccac acctacccga ctgaactcgg
     4261 gcgcgacctg cccggcgtac cgggcacgag catcatcctg gttgttgaca agaactcccc
     4321 ctaccgcacg cccgccgacc tgcgtaacac tcgcctggcg ctgccgcaca actacgtcaa
     4381 cgcccccggc gccttcctga cacgcacgct ggaggacttg gaagttccgc ccgaacagca
     4441 gttcttctca aaagtgaccc tgcgccgctt ttccaaggac gccgtaatcg acgtgctcaa
     4501 gggccatgcc gacgtagcct gcgtggacga gggcacgctc gcggcgctgg ctgactttta
     4561 cggcataaac gtgcgcatcc gcccggtggc tgtttcgccg cgctacaacc tcgatgtcct
     4621 ctttaccagc aagaacaact tagaggacta ccgcaccgag atcgaattga cccagcggca
     4681 actgactacc ctggccaagg accccgaagg ccaggaagtg ctgttctttt tcgacgaatc
     4741 cggctggttc aattaccgcc ggggcgacct cgccgtccct cgcgaacact ttggtgactt
     4801 cgttcgcttc ttgacacaga ctccagtcga cctgaaactg atgctcgctc ccaacccgcc
     4861 ggtcgatcgg caaacgtata cgatcatcgg cgacgaatga cctataataa gacgacagcg
     4921 gcgggacgcc gcctggtgag gggccgacgt cccgtcgggg aaagaggaac tttgccggac
     4981 gagatgccct cgtaacggag gaagctcgag cgagcgatca agagcacctc gtccgttatc
     5041 tcaaaactgc accacctgcc tcctccggat cggcaggcgg acacagcgca gcgcgctcct
     5101 ctcacccata aatgtacgct caaaaagatg agaggaaatg cctcgacctc gctcggcccc
     5161 cattgtgaac gcgcacggcc cgagaactaa ccggtagagg ctgcttacag agagttaaga
     5221 cggcgaggtt actcgccggt cacctgggaa ccccggctct ggctttcgcg aggacctctt
     5281 cctggactgc ccgcggcgcg acgcggtaac aggcaaactc catcgcaaac gtgccccgac
     5341 cctgtgtcag cgaccgcaga tcggttgcgt aaccaaacat gtttgccagc ggcgcctcgg
     5401 ccgcgatgag cgtcgcttcc gcgcgggctt ccgtgcccag aatcaacgcc cggcgagcgc
     5461 tcaaatcggc gactacggcg ccctggaatt cgcccggcac ctccacctcg cagcgcatta
     5521 ttggctccag tattgccggc gctgattcga gataggccgc cttgaacgcc tcccgcgcgg
     5581 caatctgaaa ggccacgtcc gacgagtcga ccgaatgcgc cgacccgtcc tccagcacca
     5641 ccttcacgcg caccacctcg tagccggcca gcggcccctt catgcgcgcc gcctgaaagc
     5701 ccttgtcgca cgacggaatg aactcggtcg gaatccgccc gccgaagacc cggttttcga
     5761 actcgtacac cttctccgcg ccttccggca gcggctccag gcgcccgatc acgtggccgt
     5821 actgcccggc cccgccggtc tgcttgcgat gccggtagtt gaacgccgca gggcgggtcg
     5881 gagtttcgcg atagctgacc ttcggcgcgc cggtaatcag tacgacaccg tattcgcgcc
     5941 gaattcgctc gcagtacacg tcaagctgca actcgcccac gccggccatg atggtctcgc
     6001 ccgtctccgc gtcggttcgc acgtgaaacg tgggatcctc gcgcgcaaaa cgccccagcg
     6061 ccttggccag cttgtcccgg tcgcgcgaat tggcggccgc cacggccaac gagataaccg
     6121 gcgccggcgc gtggatgttc tccagcgaat agctcagttc ctcgctgcag aacgtatcgc
     6181 cggtcgcaca atccacgccg agcagcgcca cgatgtcgcc cgcgcccgcc tggtcgacgt
     6241 cctcgcgttc gttggcgtgc acgcgcacca tgcgcccgat gcgcagcgtt ttgcgcgtcc
     6301 gcgaattgac gagcacgtct gtccgccgca acgtcccctg gtacacccgc gtgaacgtga
     6361 gctgcccgta cgcctcatcg accagcttga acgccatcgc caccgccggc gcgtccggat
     6421 cggccgtcat ggccacttcc gcgccctcgt tggccgtgtc cttcgcgtag tacattcggt
     6481 ccagcggcga cggcagatag cgcgccaccg cgtccatcag cggctgcacg cccttgttcc
     6541 tgtaggccga gcccatcagc accggcacga tgtcacgcgc tatcgtcagt cgccggatga
     6601 ccgattcgag gtaagcccgc tccggctgcc gctcctccag cagcatttcc atcaggtcgt
     6661 cgtcgtacag cgaaagcgtg tccagcagcg cacgccgcgc ccgctcggcc gcgtcgcgca
     6721 ggtcctcggg aatcgcctcg cgccgcacgt gctcgccgca gggcccctcg aagtagaccg
     6781 cttgcatttc gatcaggtcc acgacaccac gaaacgcctc gcccagcccg atgggtagct
     6841 gtaacggcgc ggcttcgagc ccgagcttct cctccacggc cgcgatcacg cgcgccgggt
     6901 ccgccccgac ccgatccagc ttgttgatga atatcaggcg cggcacgcca tagcgcttca
     6961 tctgccgatc gacggtcagc gactgcgact gcacgccgcc caccccacac agtacgagaa
     7021 tcgcgccgtc caacacgcgc agcgagcgct cgacttcgac tgtgaaatcc acgtgccccg
     7081 gagtatcgat gaggttgacc tggtggccga gccaatcgac cgtcgtggcg gcggccgtga
     7141 ttgtgatgcc ccgctcgcgc tccaggtcca tgtggtccat ggtggcccca tcgcccttga
     7201 cctcgcccat cttgtggatg cgcccgctgt aaaaaaggat gcgctcgctg agtgtggtct
     7261 tgccggaatc gacgtgcgcg gacacgccga tattgcggat gcgctgaatt ctgttctgtg
     7321 cagggctcgt cattgcaagg ctctcgccag tcgtattcat ctcaaggcca tcgctggttc
     7381 cggcaaagcc ggcgcgattc gggccaaagc cgtcgcgatt tctggccgcg ggttctagca
     7441 aagccgccgc ggctcatcgg cacgcggccc gtcgtcgtca cgagccagcc tgcgcttact
     7501 gcggccaagc tcatccggat cgacacagac gcgtcgcctg ggtgcaaccc acgcaggcac
     7561 cacgtcatgc cgtttatcga accagacgtt cccccgttgt ttgcggtctc gaccaagcgc
     7621 ggccgagccg ttgctggatg gggaatatcg cacgctgcgg cctagggctc gtacccttgc
     7681 cagcgaacgt gcaattctag ccggtcgcgc aggaattaca agcagagcgg t
//
LOCUS       DAOSYS010000176         7682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8126_length_7682_cov_5.963551, whole genome shotgun sequence.
ACCESSION   DAOSYS010000176 DAOSYS010000000
VERSION     DAOSYS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8126_length_7682_cov_5.963551"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="PLU99_11075"
     CDS             complement(<1..843)
                     /locus_tag="PLU99_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOO17637.1"
                     /translation="MAGIVVVVGGLAALKFLGGGDGPAAKRFPGVAAKEMAPNDAATL
                     GPALDLPSGAAAGYNVLLITLDTTRADHVGCYGATGGQTPFIDSLAAGGTRFADAVTV
                     VPMTLPAHASILTGNYPPTHGVRDNGTYRLPPETKTLATELKSAGYSTAAFIGAFVLD
                     RRYGLSRGFDVYDDLILAGPKAGPDRLNPQRRGDLVADSALRWFDEQRPAGQPFLAWV
                     HFFDPHLSYDPPEPFKSRFAGQPYDGEIAFMDQQIGRVIDKVRALGLLEKTLVVIVGD
                     HGEGL"
     gene            1215..1712
                     /locus_tag="PLU99_11080"
     CDS             1215..1712
                     /locus_tag="PLU99_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17638.1"
                     /translation="MNLTLEERIGRLERQNRWLKAAFGVLAAGAVAVLVLGQVAPSSA
                     PTGPLLLPERVRAKRFEVINDAGKPVVTLMAWEQGGWIETRDNEGRRLFDMSATDDGH
                     GLFSTYNKEGNETVSFGGVKDGTGLLAIFDGRGRKVLGAGMGEDGAGVIASYNRSQDI
                     KAVWP"
     gene            1812..2291
                     /gene="tadA"
                     /locus_tag="PLU99_11085"
     CDS             1812..2291
                     /gene="tadA"
                     /locus_tag="PLU99_11085"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOO17639.1"
                     /translation="MPGLHQDEVDRRFMREAVELARRCLDCDDVPVGALVTHGGRIIG
                     RGYNQREKLQDPTAHAEMIALTAAAAHVGHWRLEDCTMYVTLEPCPMCAGALVLARLP
                     RLVYGAADPKGGACGSLFTITEDPRLNHRVETVGGILAEPCAELLREFFRRRRALGD"
     gene            2539..3258
                     /locus_tag="PLU99_11090"
     CDS             2539..3258
                     /locus_tag="PLU99_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOO17640.1"
                     /translation="MLPQPDDRTCGPTCLHAVYRYYDDVLPLETVIAEVDHLPDGGTL
                     AVMLGCHALRRGYRATIYTYNLQMFDPTWFGSNSTDLREKLIAQAQAKAIEKLRLATE
                     KYLEFLDLGGAVRFEDLTTRLIRRYLVRGVPVITGLSTTYLHRSPREYGPRDEEDDVR
                     GYPAGHFVVFHGYDRRKRTVLVADPMHPNPLGPGQNYAVSIERVLGAVFLGVLTYDAN
                     LLIIEPPPVHKGQTRVPTDRG"
     gene            3236..>4677
                     /locus_tag="PLU99_11095"
     CDS             3236..>4677
                     /locus_tag="PLU99_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HOO17641.1"
                     /translation="MFPLIVVDNPKTWPLHIPGASVIAARAYLTDPVYSELRNARVFN
                     LCRSYRYQSTGYYVSLLAMARGHKPLPNITTIQDMKSGTIIRLASDDLEDVLQASLKP
                     IHSHAFTLSIYFGRNLAKRYDALSAHLFRLFQAPFLQAEFVHDEEDGWRLRNISPLAA
                     NEIPEAHKEFAIEAARQFFAQKRYPAQRKSDVLFDLAILVNPDNPKPPSNRRALKRLV
                     DAAKRAHFSVELIDNDDFGRIGEFDALFIRETTAVNHFTYRFARRAVAEGLVVIDDPD
                     SITKCTNKVYLAELLDQHSIPRPKTLIIHKDNREAIRPYIGLPCVLKQPDSSFSMGVT
                     KVDTEEELNDTVDRLLADSDLIIAQQFIPTPFDWRITVLDRQLLFACKYHMAPHHWQI
                     IKPGKNGDYDAGEVEPVPPEQVPPQVLSTAMRATKLIGDGLYGVDLKQIGNQVYLIEI
                     NDNPNIDAGYEDRLMGDDLYDRVMQVFLRR"
     assembly_gap    4678..4777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4778..6027
                     /locus_tag="PLU99_11100"
     CDS             <4778..6027
                     /locus_tag="PLU99_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HOO17642.1"
                     /translation="TYHLFERYGVELEYMIVARDDLRVRPVADELIRQACGEFASDVE
                     RGPITWSNELVLHVIELKTTDPATSLAGLAPAFQAEIQAMNGMLAPQGAQLMPTSMHP
                     LMDPNTETRLWPHEYNPVYATFDRIFNCRGHGWANVQALHLNLPFAGDEEFGRLHAAI
                     RLVLPILPALAASSPVAEGRFTGWADTRMEYYRNNSRRVPLVAGAVIPEPVFTPQEYR
                     ERILAAMYEAIAPLDPEGVVQDEWLNARGTIARFVRDTFEIRVVDIQECPAVDLALLG
                     LVVAVLRGLVSERWSDWRQQKSWDVAPLEAIMLATIRDAERAVIRDSAYLRIFGLDEK
                     SCTAGRLWRHLFEQALASELPAEFGTVLAAVMQHGSLATRIGRALGPAPDRARIAAVY
                     ESLCACLQRGVPFGLDPDGSQKS"
     gene            6218..7105
                     /gene="sucD"
                     /locus_tag="PLU99_11105"
     CDS             6218..7105
                     /gene="sucD"
                     /locus_tag="PLU99_11105"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOO17643.1"
                     /translation="MSILVDRNTRLICQGITGKAGAFHTGQCLEYGTQVVGGVTPGKG
                     GTTSEHGLPVFNTVYEAVAKTGANATMIFVPAPYAAAAAMEAADAGIRLVVLITEGVP
                     TLDMVKARKFLDDKGTRLIGPNCPGIITPGQCKIGIMPGYIHKPVERGTRNVGLISRS
                     GTLTYEAVWQCTQRGIAQTTCIGIGGDPVKGLNFIELLEMFNADPDTHGIVMIGEIGG
                     TDEEKAAEYIKQHVRKPVVAFIAGQTAPPGRRMGHAGAIISGGEGTAQSKIAALREAG
                     ITVSDSPATIGDAVVKALG"
     gene            7184..7588
                     /gene="rsfS"
                     /locus_tag="PLU99_11110"
     CDS             7184..7588
                     /gene="rsfS"
                     /locus_tag="PLU99_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOO17644.1"
                     /translation="MSEAEKLEEEPQSAANAEAREFAIAVARIAAENKTTEVEVLDLR
                     GLSGFADYFVLGTGTSERQMHAVVDLVEEHARAIGRTPFRVADSREAHWILTDYVDVV
                     LHLFDAEHRDYYDLSNLWGDAPRVPWEAQQQS"
BASE COUNT         1384 a         2340 c         2510 g         1348 t
ORIGIN      
        1 caggccctct ccatggtcgc ccacgattac cacgagcgtt ttctccagca gccccagcgc
       61 gcgaaccttg tcgatcacgc gcccgatctg ctggtccatg aatgcgatct cgccgtcgta
      121 gggctggccg gcaaaacgtg acttgaaggg ctccggcggg tcgtacgaca gatgcggatc
      181 gaagaaatgc acccacgcca ggaaaggctg cccggccggt cgttgctcgt cgaaccagcg
      241 cagggccgaa tcggcgacca gatcgccccg acgttgggga ttgagccggt caggaccggc
      301 ttttggtcca gcgaggataa ggtcgtcgta aacatcgaag ccgcggctca gcccgtagcg
      361 ccgatcgagc acaaacgccc cgatgaacgc ggcagtgcta tagccggccg atttcagctc
      421 agtggccagc gtcttggttt ccggcggcag gcgataggtt ccgttatcgc gcacgccatg
      481 cgtcggcggg tagttgcccg tcaagatgga ggcatgggcc ggcaaggtca tcggaacgac
      541 ggtgacggcg tcggcgaagc gcgtcccgcc cgccgccagg ctgtcgatga atggcgtttg
      601 cccgccggtg gccccgtagc agccaacgtg gtcggcgcgc gtagtatcga gagtgatcag
      661 cagaacgttg tagccggccg cggcgccgct gggtaagtcg agagctggcc caagcgtagc
      721 cgcgtcgttt ggcgccattt ctttcgcggc gacgccgggg aaccgcttcg ccgccggtcc
      781 gtcacccccg cccaggaact taagtgcggc cagtccgccc accacgacga ctatcccggc
      841 cacggcccaa atcggccaac gtcgcggctt tgcggatttg gcgggcttgg cccgcacagc
      901 gatggaagcc ttcttgcctg ggccggtttt ctgctttctg cgggggttca gaacacttct
      961 ccctactcga gttagccgct ctagcgaacc agacgggtcg gaatcgcccg atccgggcgc
     1021 gccgccgtaa gaaccgctcc cacagcgact tagctgaatg cgcttcgtac catcttaatc
     1081 tcggatgcgg ccgcacccaa gcgtattaga tgccgccggg cgtccggctc cggctctctg
     1141 tgaggccgct ggacctttcc gcggcacggg attccaatta ggagcggccc cgcacgggcc
     1201 gtcaggagcg tactgtgaat ctgactctgg aagaacgaat cggccgcctg gagcgacaga
     1261 accgttggct gaaggcggcc ttcggtgtgt tggccgcagg tgcagttgcc gttctcgtac
     1321 tgggacaggt cgcgccgagc agcgcgccca ccgggccgtt gttgctgccg gagcgggtcc
     1381 gggccaagcg ctttgaggtc atcaatgacg ctggcaagcc ggtcgtcacg ctcatggcct
     1441 gggagcaggg cggctggatt gagacgcgcg ataacgaggg ccgccgcttg ttcgacatgt
     1501 cggcgacaga cgatgggcac ggcctctttt cgacctacaa caaggaaggc aacgaaacgg
     1561 tttccttcgg cggcgtaaag gatggcactg gcctgctggc catattcgat ggccgcggtc
     1621 gcaaggtcct gggcgccggt atgggcgagg acggggcagg cgtgatcgcc tcgtataacc
     1681 gtagtcagga tattaaggcg gtttggcctt aggcagattg ggaccgcgct aaccgcgccg
     1741 ggggcgccag cgagccgctg ggctgacgat tgaccgccga gatctaaaaa ctactagttt
     1801 caggggtagc catgcccggt ttgcaccagg acgaagttga ccgccggttc atgcgggagg
     1861 cggtggaact agcgcgccgc tgcctggact gcgacgacgt cccggtcggc gcgctggtca
     1921 cgcacggcgg gcgcatcatc gggcgcggtt acaaccagcg cgaaaagctg caggacccga
     1981 ccgcccatgc ggagatgatc gccctgacgg ccgcggccgc acacgtaggt cattggcggc
     2041 tcgaagactg tacgatgtat gtcacgttag agccctgccc gatgtgcgcc ggggcgctgg
     2101 tgctggcccg gcttccgcgc ctggtctacg gcgccgccga tccaaagggc ggcgcgtgcg
     2161 gttcgctgtt tactattacg gaggatccgc ggctgaatca ccgggtcgag acggtgggcg
     2221 ggattttggc cgaaccatgc gcggagttgc tgcgtgagtt tttccggcgg cggcgggcgc
     2281 tgggggattg aaaaggcggc aaggcaaggc gcgggacagt aaagccgaac gaatcagtct
     2341 ctgccgccgg gcgaggatac cttctgccgc gggtgaggtc aattccgtgg cggcacgccc
     2401 cggctcaggt cgatattatt tctggcagcg gcacggctgg actggacggg ctgaaggccg
     2461 gataatacgt ccccttttgg gggccgctgt caaaagttag acagccgaag ctgaatgcat
     2521 cgccaacttg acttattaat gttgccacag ccggacgatc gcacttgcgg tccgacgtgc
     2581 ctgcacgccg tctatcgcta ttacgatgac gttttgccgc tggagacggt gattgcggag
     2641 gtggaccacc tgccggacgg gggcacgctg gcggtaatgt tgggctgtca tgcgttgcgg
     2701 cgcggctacc gcgcgacgat ttacacgtat aatctgcaaa tgttcgatcc gacctggttc
     2761 gggtcgaact cgacggacct gcgtgagaag ctgatcgcgc aggcccaggc gaaagcgatt
     2821 gagaagctgc ggttggctac ggagaagtac ctcgaattcc tcgacctggg cggtgcggtg
     2881 cggtttgagg acctgacgac gcgcttgatt cggcgctatc tggtgcgggg cgttccggtc
     2941 attacggggc tgagcacgac ctatctgcac cgcagcccgc gcgaatatgg accacgtgac
     3001 gaggaagacg acgtccgtgg ttacccagcc gggcattttg tcgtctttca tggatatgac
     3061 agaaggaagc gaacggttct ggtggctgac ccgatgcacc cgaatccgct tgggccgggc
     3121 caaaattacg ccgtgtccat cgagcgcgtg ctcggcgcgg tatttctggg tgtactgacg
     3181 tatgacgcaa acttgcttat catcgagcca ccgcccgttc ataaagggca aacccgtgtt
     3241 cccactgatc gtggttgaca atccgaagac ctggcccctg cacattccgg gcgcatcagt
     3301 catcgcggcc cgggcgtatt tgacggaccc ggtctactcg gagctgcgca acgcgcgcgt
     3361 gttcaacctc tgccgctcgt accgttacca aagcaccggc tattacgttt cgctgctggc
     3421 gatggcccgc gggcacaagc cgctgccgaa catcacgacc atccaggaca tgaagtcggg
     3481 caccatcatc cgcctggcct cggatgacct ggaagacgtg ctgcaagcca gcctcaagcc
     3541 gattcactcg cacgccttca ccctcagcat ctacttcggg cgcaacctgg ccaaacgcta
     3601 tgatgcgctc agcgcgcacc tgtttcgcct gttccaggcg ccgtttctgc aggccgagtt
     3661 cgtgcacgac gaggaagacg gctggcgctt gcggaacatc agcccgctcg cggcaaacga
     3721 aatccccgaa gcgcacaagg aattcgccat cgaggcggca cgccagttct ttgcccagaa
     3781 gcgctacccc gcgcagcgca agagcgacgt gctgttcgac ctggccatcc tggttaaccc
     3841 cgacaatccc aagccgcctt cgaaccgccg ggcgctgaag cggctggtcg acgcggccaa
     3901 gcgcgcccac ttcagtgtcg agctgatcga taacgacgat ttcgggcgca tcggtgaatt
     3961 cgacgcgctg ttcatccgtg agacgaccgc ggtgaatcac ttcacctatc gtttcgcgcg
     4021 ccgcgcggtg gccgaaggac tggtggtcat cgacgacccc gattcgatca ccaaatgcac
     4081 gaacaaggtc tacctggccg agctgctcga ccaacacagc atcccgcgtc ccaaaacgct
     4141 catcatccac aaggacaacc gtgaggccat ccgcccctac atcggcctgc cgtgcgtcct
     4201 caaacagccg gacagttctt tctccatggg cgtcacgaag gtcgacacgg aagaggagct
     4261 gaatgacacg gttgaccgtc tgctggccga ctcagatttg atcatcgcgc agcagttcat
     4321 cccgacgccg tttgattggc gcatcaccgt cctcgatcgg cagttgctgt tcgcctgcaa
     4381 atatcacatg gcgccccatc actggcaaat tatcaagccc ggcaagaacg gcgactacga
     4441 cgcgggggag gtcgagccgg tgccgcccga gcaggtgccg ccgcaggtgc tcagcaccgc
     4501 catgcgggcc accaagctga ttggcgacgg cctgtacggc gtagacctta agcagatcgg
     4561 caaccaggtt tatctcatcg agatcaacga taaccccaat atcgacgcgg gctacgagga
     4621 ccgcctgatg ggcgacgacc tgtacgaccg cgttatgcag gtcttcctgc gccgcatnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa cttatcacct cttcgagcgc
     4801 tacggcgtcg agctggagta catgatcgtg gcgcgcgatg acctgcgcgt ccggccggtc
     4861 gccgacgaac tcattcgcca ggcctgcggc gagttcgcct ccgacgtgga gcgcggcccg
     4921 atcacctggt cgaacgaact cgtcctgcac gtcatcgagc tgaagacgac cgatccggcc
     4981 acgtcgctgg ccggcctggc ccccgcgttt caagccgaga tacaggccat gaacggcatg
     5041 ctcgcgccac aaggtgcgca gctcatgccg acctcgatgc acccgctgat ggacccgaat
     5101 accgagacgc gcctctggcc gcacgaatac aacccggtct acgcgacctt cgaccgcatt
     5161 ttcaattgcc gcggacatgg ctgggccaac gtgcaggcgc tgcatctgaa cctgccgttc
     5221 gccggcgacg aggagttcgg ccggctgcac gctgcgatac gtctggttct gccgatctta
     5281 ccggcgctgg ccgccagctc accggtcgct gagggacgct tcacgggctg ggctgatacg
     5341 cgcatggagt actaccggaa caactcgcgc cgcgtgccgt tggtggccgg cgccgttatt
     5401 cccgagccgg tttttacgcc gcaggaatac cgggagcgca ttctggccgc gatgtatgag
     5461 gcgatcgcgc cgctggaccc ggaaggcgtg gtgcaggacg agtggctcaa cgcccgcggc
     5521 acgatcgccc gttttgtgcg cgacacattc gagattcgcg tcgtggacat ccaggaatgc
     5581 ccggccgtcg acctggcgct gttgggtttg gtcgtcgcgg tgctgcgcgg cttggtcagc
     5641 gagcgctgga gcgactggcg gcagcagaag agctgggacg tggcgccgct ggaagcgatc
     5701 atgctggcca cgatccgcga cgcggaacgc gccgtcattc gggattccgc ctacctgcgc
     5761 attttcggcc tcgatgagaa gagctgcacg gccggccggt tatggcggca cctttttgag
     5821 caggctctgg cgtccgagct gccggccgaa tttgggacgg tgctcgcggc ggtaatgcag
     5881 cacggctcac tggccacgcg catcgggcgg gccctggggc cggcgccgga ccgggcgcgc
     5941 atcgcggcgg tttacgagag tttgtgcgct tgcctgcagc gcggcgtccc gttcgggctc
     6001 gatccggacg gatcgcagaa atcgtgagtg aggtggctcg gcgaaggcag atgtaggggg
     6061 gcaccgtccg cgacggcttt cgcttaccgc cgccctttgc cataatcgcc ggttcgacaa
     6121 aaaggcccgc cacaggtggc ggcagaagac atgaccggcc gggccgaaaa ccggtccggc
     6181 aggaaagaca ctaaagcgag aatcgaggtc ctggcgcatg agtattttgg tcgaccggaa
     6241 cacgcggctg atttgtcagg ggatcaccgg aaaggcgggg gctttccata ccgggcagtg
     6301 cctggagtat ggcacgcagg tggtgggcgg cgtgacgccc ggcaagggcg gcacgacctc
     6361 cgagcacggt ctgcccgtgt tcaacacggt ctatgaggcg gtcgccaaaa ccggggcgaa
     6421 cgcgaccatg atcttcgtcc cggcgccata cgcggctgcg gcggccatgg aggcggcgga
     6481 cgccggcatt cggctggtgg tgctgatcac cgagggcgtg ccgacgctcg acatggtcaa
     6541 ggcccgcaag ttcctggacg acaagggcac gcggctgatc ggcccgaact gtccgggcat
     6601 cattacgccg gggcagtgca agatcggcat catgccgggc tacattcaca agccggtgga
     6661 gcgcggtacg cggaacgtcg gcctgatctc acgcagcggc acgctgacgt acgaggcggt
     6721 ttggcaatgc acgcaacgcg gcatcgcgca gacgacgtgc atcggcatcg gcggcgatcc
     6781 ggtcaagggc ctgaacttca tcgaattgct cgagatgttc aacgccgacc cggacacgca
     6841 cgggatcgtc atgatcggcg agatcggcgg cacggacgag gagaaagcgg ctgagtacat
     6901 caagcagcac gtgcgcaaac cggtggtggc gtttatcgcc ggccagaccg ctccgccggg
     6961 gcgccgcatg gggcacgccg gcgccatcat ttccggcggt gagggcacgg cgcaatccaa
     7021 gatcgcggct ctacgggaag ctggtatcac ggtcagcgac agcccggcca ccattggaga
     7081 tgcggtggtg aaggcccttg gctgacgcgc gcttcaagtg tcgcgtttca agaaaggagc
     7141 gcgcggggct gctggattgg gttttgaccg tacaattagc tgcatgagcg aagcggaaaa
     7201 actcgaggaa gaaccacaga gtgcggcgaa cgccgaggcg cgcgagttcg cgatcgccgt
     7261 ggcgcgcatc gcggccgaga acaagacgac ggaggtggag gtcctcgatc tgcgcggact
     7321 gagcgggttc gcggattatt tcgtgctcgg gacggggacc tctgagcggc agatgcacgc
     7381 cgtggtggat ctggtggaag agcacgcccg cgccatcggt cgcacgcctt tccgcgtcgc
     7441 tgattcgcgc gaggcgcact ggattttgac cgattacgtc gatgtcgtgc tgcacttgtt
     7501 cgatgccgag caccgcgact attacgattt gtcgaacttg tggggagatg cgccccgcgt
     7561 gccatgggag gcgcagcagc aatcgtaacc ggggcgaaag cgaggtccta tgcgcagctt
     7621 cgttgccgtc gacctggctc ctgagcttcg ccggccgcta gtgcggctgt tgcaggagct
     7681 gc
//
LOCUS       DAOSYS010000177         7670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8143_length_7670_cov_4.526461, whole genome shotgun sequence.
ACCESSION   DAOSYS010000177 DAOSYS010000000
VERSION     DAOSYS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8143_length_7670_cov_4.526461"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLU99_11115"
     CDS             complement(<1..793)
                     /locus_tag="PLU99_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17645.1"
                     /translation="MQLTQAAPAYDESLVQVLEGTEHVRKRPSMYIGDTGLRGLHHLV
                     YEVVDNSIDEAMAGACRNISVVIKADGTVSIEDDGRGIPVGMKEEYNMSAVELCLTKL
                     GGGAKFDRNAYKVSGGLHGVGVSVVNALSERMEVQVQRDGHVWVIGFERGQTTRPLEA
                     ISRSNRTGTRVDFKPDREIFRETDFHYDILEKRLRELAYLNSGLRIRLTDERSDKQAE
                     FFFEHGIRQFVEHLNEGKQAQHPAVYFHREDPEQRLICEIAFQYTD"
     gene            complement(813..1181)
                     /locus_tag="PLU99_11120"
     CDS             complement(813..1181)
                     /locus_tag="PLU99_11120"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOO17646.1"
                     /translation="MTRRTAYEIGQRRFQQAVANRRQYKVQPLGAALAEFFAGPAAEP
                     CEEALVHEAWREAVPESVRHSTRVETFTGGVLCVKVDDPVTRFSLLGQRRKLQQRLRR
                     TLPHLRAIRIVLDENGSGKQ"
     gene            complement(1178..2296)
                     /gene="dnaN"
                     /locus_tag="PLU99_11125"
     CDS             complement(1178..2296)
                     /gene="dnaN"
                     /locus_tag="PLU99_11125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOO17647.1"
                     /translation="MKTTLQRQEFLDALTAVSSLTSGRSPKPILACTKLSATADGMEL
                     AATDGEAALRLGISALEVDTPGEVVVNAERLLSIVRELADVEITLQTDERQCIIRGQG
                     SEFKIYMQNLADFPPVAGFDNEPDVVLDGRELRRMIGLTLYAAARETSRYAINGVLWD
                     KQAKRLYMVATDGRRLARAGGNVRKSDSAAFSVIVPTKALSVFERVFTSPKTDDEWLV
                     DVKVTPNQLILHSLNSVLSTALVEGRFPKYEDVIPQEGNRQAVVDRAEFYGAVKRAAL
                     LTTEDSRAVRLAFSREQLVISSQSPEQGEARIEVPIEFKGEDIAIGFNPAFLSDALKV
                     LPFEQVHIEMQEPFRPGVITGGDKNEFLYVVMPVSLAS"
     gene            complement(2651..3961)
                     /gene="dnaA"
                     /locus_tag="PLU99_11130"
     CDS             complement(2651..3961)
                     /gene="dnaA"
                     /locus_tag="PLU99_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOO17648.1"
                     /translation="MSDEPLDWSALLQAVRTDYPTLYRAWFESLEPGRLIGGELRVSV
                     ADPAWLHYLREHCAQVFSHTAMELTGHLVSVRFESGEESVLDDPVRQQRQLRRLPLNP
                     DYTFAEFVVGPSNRLAHAAGLAVCSQPGVLYNPLFIHGASGLGKSHLLQAICAELLRS
                     SPDANVIYVACDTFVSEYVSAIEAGDLHTFRNSVRQADALLIDDVQFLANRESSQEEL
                     FHTFNALYQERRQIVLSADSPPSDIPNLEDRLVSRFNWGLVAQIDPPTRETRQAILQK
                     KARLRGYEIPADVIDYVAEHVDSNIRTLEGTLTKLITQHKHCDKPLTLATAKEVLPPP
                     APVTKPLQVSDILQVVSQHFGIRLQDLLGRRRTRSVSYPRNIAIYLARKMTMLSLEEI
                     GMHFDGRDHSTVLHAERAIQQESERDSATQETLAILTKQLLSRT"
     gene            complement(4001..5215)
                     /gene="truD"
                     /locus_tag="PLU99_11135"
     CDS             complement(4001..5215)
                     /gene="truD"
                     /locus_tag="PLU99_11135"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HOO17649.1"
                     /translation="MIKAQHSDFVVHEEPLYTPSGEGTHTYFLIEKSGISTLQAVNVM
                     ATALNVGRRDIGFAGMKDAHAVARQWLSVEHVAPKIIEGLELPRIRVLEVTRHTNKLR
                     LGHLRANRFVVKVRQTEPQRVSEVQAALDELARRGVPNYFGPQRFGTRGTTWEIGQAI
                     VQNQPDRALDVLLGRPTPADHGAIRRARKLYDLGKYADAVRQWPGVFADERRALRVLA
                     RTGNRRRAWGAVGRRTKLFYLSAYQSYLFNQVVAQRLPHGLDHLWEGDLAWIHAKGAV
                     FQVLDPVVEQPRADAFEISPTGPLFGNRMSSPSGHPAEMEARVLAAQGLPPAAFSTGP
                     GRSQGGRRPLRFRPEMGNIQLGADDGGPYLELRFTLPPGCYATCLLRELFSQAERAPS
                     ADDLDGTEEQAG"
     gene            5489..6382
                     /locus_tag="PLU99_11140"
     CDS             5489..6382
                     /locus_tag="PLU99_11140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOO17650.1"
                     /translation="MSKPTARLGKGLSALITPRAPVSQPTVARGSEPATTISVEAIRP
                     NPRQPRKDFDDAAITQLANSIRTKGVLQPVIVRPAPDGGYELVAGERRLRAAKLAGLT
                     QVPAIVRDVSDADSVEIALIENLQREDLNPLERAAAYRQYLDTFKSTVEELASRLGES
                     RPNISNYLRLLDLAEPVRKLLASGELSMGHARAIASIENPERQLALAMLCIRRNLSVR
                     QLEVLARQQTNTVTKVAERVPLQNRHIAEVEQALARGLGLPVKLRPGRKKNSGRVVIT
                     YRNLDEFDLIANKIGGHCKLE"
     gene            6387..7241
                     /gene="amrB"
                     /locus_tag="PLU99_11145"
     CDS             6387..7241
                     /gene="amrB"
                     /locus_tag="PLU99_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOO17651.1"
                     /translation="MPLRLPIAAGRFYPSDAPECVALLNEMQASVEPVPAVGAIAPHA
                     GWIYSGRTAMRSIVSLAAKSPETVIVFGAVHVLTHADASLFSEGTWQTPLGLVAVDSD
                     LGGLIRRIDHVQDDPEAHRFEHSIEVLLPMIQHVLPEARIVPLMVKPGRRAEQIGRDC
                     ARAAKESGRRVAFLASTDLTHYGPAFGIEPVGRGAAGVKWAKEVNDRRLIKLIAVGRA
                     LEVLAEAEQSRSACGAGAVAALLGAMGELGAVEFRELEHTSSAEVEWAAGGGAAVNSV
                     GYLAGVFL"
     gene            complement(7327..>7670)
                     /locus_tag="PLU99_11150"
     CDS             complement(7327..>7670)
                     /locus_tag="PLU99_11150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO17652.1"
                     /translation="RGRIPIAYGELNVLDSQRGLYWLGHLLVAPQRRRCGVGAALTSA
                     LLNRAFSNYAAAAVTLVVFPDNAPARACYYRAGLREDGWEVHYLPPYQRRVRLLRMSI
                     KRPAQLAVPSF"
BASE COUNT         1415 a         2383 c         2396 g         1476 t
ORIGIN      
        1 cgtcggtgta ctggaacgcg atctcgcaaa tcagccgctg ctccgggtcc tcgcggtgga
       61 agtacacggc cgggtgctgg gcctgcttgc cttcgttaag gtgctccacg aactggcgga
      121 tcccgtgctc gaagaagaac tccgcctgct tgtcactgcg ctcgtccgtc agcctgatgc
      181 gcagcccgga attgagatac gccagttccc gcaaccgctt ttcgagaatg tcgtaatgga
      241 agtcggtctc gcggaaaatc tcgcgatccg gcttgaagtc cacgcgcgtg ccggtgcggt
      301 tactgcggct aatcgcctcg agggggcgcg tggtttggcc gcgttcgaac ccgatcaccc
      361 agacatgccc gtcgcgctgc acctgcactt ccatgcgttc tgacagcgca ttgaccacgc
      421 ttacgcccac gccatgcagc ccgccggaga ccttgtaggc gttgcggtcg aatttcgcgc
      481 cgccgcccag tttggtcagg cacagctcga cggctgacat gttgtattct tccttcatgc
      541 cgaccgggat gccgcgcccg tcatcctcga tgctgacggt gccgtccgcc ttgattacca
      601 cgctgatgtt gcggcacgcc ccagccatgg cctcgtcgat tgagttgtcg actacttcat
      661 agaccaggtg gtgcaggccg cgcaggccgg tatcgccgat gtacatgctc ggccgcttgc
      721 ggacatgctc ggtgccctcc agcacctgga ccagactctc gtcatacgcc ggcgccgcct
      781 gtgtgagctg cacgctaggg ccctcagcca ggttattgtt ttccagaccc attctcgtcc
      841 aagacaatcc ttatggctcg caagtgtggc agtgtgcgcc gcagccgctg ctgtagcttt
      901 cgccgctgtc ccagcagtga aaagcgcgtc actgggtcat cgaccttgac gcacaagacc
      961 ccgccggtga aggtctctac ccgcgtcgaa tgccgcacgc tctccgggac cgcctcgcgc
     1021 caggcctcgt gcaccagcgc ctcttcgcac ggttccgcag ccggccccgc aaagaactcg
     1081 gctagcgccg cacccagtgg ctgcaccttg tactggcgcc ggttcgcaac ggcctgttgg
     1141 aaccggcgct gcccaatctc gtaagctgtc ctgcgcgtca tgatgctagc gaaaccggca
     1201 tgaccacgta caggaactcg ttcttatcgc cgcctgtgat tacgcccggc cggaagggct
     1261 cctgcatctc tatgtgtacc tgctcaaacg gcaggacctt aagcgcgtcg cttaagaaag
     1321 cggggttgaa cccgatcgcg atgtcctcac ccttgaattc gatcggtacc tcgatgcgcg
     1381 cctcaccctg ttcgggcgat tgcgatgaga tcaccagttg ctcgcgactg aaggccagcc
     1441 gcaccgcccg cgagtcctcg gtcgtgagca gcgccgcccg cttaacggct ccgtagaact
     1501 ccgcccggtc caccaccgcc tggcgattac cttcctgcgg aatgacgtcc tcgtactttg
     1561 gaaaacgacc ctcaaccaga gcagtcgaaa gcacgctgtt aagagagtgg agaatcagtt
     1621 gattaggcgt caccttgacg tcgaccagcc actcatcatc cgtctttggc gaagtgaaaa
     1681 cacgctcaaa gacgctcagc gccttagtcg ggacgattac gctgaacgcc gcgctatccg
     1741 acttgcgaac attcccacct gcccgggcca gccgcctacc gtccgtggca accatgtaca
     1801 gccgcttggc ctgcttgtcc cacaacacac cattgatagc gtaccggctg gtttcgcgcg
     1861 ccgccgcgta gagcgtcaag ccgatcatgc gccgcagctc ccgcccgtcc agcacgacat
     1921 ccggttcgtt gtcaaagccc gccaccggtg ggaagtcggc caggttttgc atgtagattt
     1981 tgaactcgct gccctgacca cggatgatgc attggcgctc gtcggtctgg agcgtgatct
     2041 ccacgtcggc gagctcgcga acgatgctta gcaggcgttc cgcgtttacg accacctcgc
     2101 caggagtgtc gacctccagg gctgaaatcc caaggcgcaa agcggcctcg ccgtccgttg
     2161 cagccagttc catgccgtcg gccgtagcgc tcagtttggt gcatgccaat atgggcttgg
     2221 gcgaacgtcc gctggttagc gagctcaccg cggtcaaagc gtctaggaat tcctgtcgct
     2281 ggagcgtcgt tttcatggtt taaacctgcc gaaacggtat caaggcctcc gcagcgaacg
     2341 ccggcttgca cgggtgggcc gctacaagat ctggtcaccc gctcgggcgg ctaccccaat
     2401 attttacggt ttttgccgca ggccgccagc gcttggccgg agtcagtgaa agcgccgaac
     2461 atcaggaaat tgatgttcgc accggatact gttaattacc ttctcagcaa gagcttcgta
     2521 gtcgtaaccg tatgagctgt caatcctgtt atcaggcaac gtgaatcgcc gtaagtcgtg
     2581 tcccgaacta attttatgca tggcaggtcg gtatttcacg ctgttaatcc ggcgttggtt
     2641 tctgcgtcac ttaggtgcgt gagaggagct gtttggtcag aatcgccaag gtttcctgtg
     2701 tggcgctgtc gcgttcgctt tcctgctgga tggctcgttc cgcgtgtaac acagtcgagt
     2761 ggtcgcggcc gtcaaagtgc atgccgattt cctcgaggct gagcatcgtc atttttcgcg
     2821 ccaggtaaat ggcgatgttt cgcgggtagc tgaccgaacg cgtgcggcgc cggccgagga
     2881 ggtcctgcaa gcgtatgccg aagtgttggg acacgacttg caggatgtcg cttacctgca
     2941 ggggcttcgt taccggagcc gggggtggca ggacttcttt ggcggttgcc agcgtaagcg
     3001 gcttgtcgca gtgcttgtgc tgggtgatga gcttggtcag cgttccttcc agcgtgcgaa
     3061 tgttggaatc gacgtgctcc gccacgtagt cgatgacgtc cgcgggtatt tcgtagccgc
     3121 gtagccgggc ctttttctgg aggattgcct gccgggtttc gcgcgtgggt ggatcgattt
     3181 gcgcgacgag gccccagttg aagcggctaa cgaggcggtc ttcgaggtta ggtatatctg
     3241 acggcggcga gtcagccgag agaacgattt ggcgtcgttc ttgatacagg gcgttaaagg
     3301 tgtggaacag ctcctcctgg ctggactcgc gattcgcgag aaactgaacg tcgtctatga
     3361 gtagggcgtc cgcctggcga acgctgttcc ggaatgtatg caggtcgccc gcttcgatgg
     3421 cgctgacgta ctcgctcacg aacgtatcgc acgccacgta aatcacgttc gcatccggcg
     3481 acgagcgaag cagttcagca catatcgcct gcaaaaggtg cgatttgccc aaaccggacg
     3541 caccgtgaat gaacagcggg ttgtacaaga cgccgggctg gctgcaaacc gccaggccgg
     3601 cggcatgcgc gaggcgattg gacggaccga ccacgaactc cgcgaacgtg taatccggat
     3661 tcaacggcag ccgccgcaac tggcgctgct ggcgcaccgg gtcatccagg acgctctcct
     3721 cgccgctctc aaagcgaaca gaaacaaggt gcccggtcag ctccatggcg gtgtgcgaaa
     3781 acacctgagc acagtgctct cgcaggtaat gcaaccacgc cggatccgct acgctcactc
     3841 ggagctcccc gccgatcagc cgccctggtt caagcgattc gaaccatgca cggtatagcg
     3901 tggggtaatc ggtacgcacc gcttgcagca gcgcgctcca atcaagaggc tcatctgaca
     3961 tggtgaaatc cgtctgcgca gccgcccgcc cagcacccgc tcagccggct tgctcctcag
     4021 ttccgtccaa atcgtctgcc gatggggcgc gctcggcctg cgagaaaagt tcgcgcagca
     4081 ggcacgtggc gtaacagccc ggcggcagtg tgaaacgtaa ctcaagatag ggaccgccgt
     4141 catccgcgcc aagctgaata ttgcccattt cggggcggaa gcgcagcggc cgtcgaccgc
     4201 cctggctgcg gcccggcccc gtgctgaacg ctgccggtgg caatccctgc gcggccagta
     4261 cgcgcgcttc catttctgcg gggtggcccg acggactgga catgcggttt ccgaagagcg
     4321 ggccggtggg gctgatttcg aaggcgtcgg cacgcggctg ttcgacgacg gggtctaaaa
     4381 cctggaagac ggcgcccttg gcatggatcc aagccaggtc gccctcccag aggtggtcga
     4441 ggccatgtgg gagccgctgg gccactactt ggttgaacag gtaggactgg tacgcggaga
     4501 gataaaagag cttcgtgcgc ctgccgaccg caccccaggc ccggcgccga ttacccgtac
     4561 gggccaagac gcgtagcgcg cggcgctcgt cggcgaacac gcccggccac tgccggacgg
     4621 catcagcata cttgccgaga tcgtaaagct tccgcgcgcg ccggatggct ccgtggtcgg
     4681 caggggttgg gcgtccaagc aggacgtcca aggccctgtc cggttggttc tgtacgattg
     4741 cctgcccaat ttcccacgtc gtcccacgag ttccgaagcg ttgcgggccg aagtagttcg
     4801 gcacaccgcg gcgcgccagc tcgtcgagcg ccgcctggac ctctgaaacc cgctgcggtt
     4861 ccgtctggcg cactttgacg acgaagcgat tggcgcgcag gtggccgagg cgaagcttgt
     4921 ttgtgtgccg cgtcacctcc agcactcgga tgcgcggtaa ttcgagaccc tcaataattt
     4981 ttggggctac gtgttcgacc gagagccact gccgggccac ggcgtgggcg tctttcattc
     5041 cagcgaagcc gatgtcgcgc cggccgacat tcagggcggt ggccatgaca ttcacggcct
     5101 ggagggtgct tatgccgctc ttttcaataa ggaagtaagt gtgtgtaccc tcaccgctgg
     5161 gggtgtagag cggctcctcg tggaccacga agtcgctgtg ctgcgcttta atcacgcctg
     5221 gcagccggct gaacccgtgc aggaggcacg gaagtgcgaa gctcgtgtcc gtttgtggaa
     5281 catctgtttc tggcacggtg tgagcgtacg cgagaaccca gccggggtct agaagtcggc
     5341 gatcgggtta atcattaggc gggcgggctc ggcaatcaga tatcgagacc tttaactcgc
     5401 ctaatcgctc cgtttcacgt ggaacgctcg tacgccggct tgacataaac taagcgcggc
     5461 gtaacctgcc caataattgg aggtgaacat gtccaagcct accgctcggc tcggtaaagg
     5521 attgagtgct ttaatcacgc cgcgcgcgcc cgtctcccaa ccgaccgtgg cacgaggcag
     5581 cgaaccggcc accacaatct ccgttgaggc gattcgacct aacccgcgac agcctcgaaa
     5641 agacttcgac gacgcggcca ttacgcagct cgccaattcc atccgaacca agggcgttct
     5701 ccagcccgtg attgtacgac ctgcgccgga cggcgggtac gagctcgtcg cgggcgaacg
     5761 gcggcttcgt gccgccaaac tggccggctt gacccaggtg cccgccatcg tccgggacgt
     5821 gagtgacgcc gactccgttg agatcgcgct catcgaaaac ctccaacgcg aagacctcaa
     5881 tccgctcgaa cgagccgcgg cttaccggca gtacctcgat acgttcaaga gcaccgtcga
     5941 agagctggcc agccgcctgg gcgagagccg gccgaatatc tcgaactacc tccgccttct
     6001 ggacttggcc gagccggtgc gcaagctgct cgcctccggt gagttaagca tgggacacgc
     6061 cagagctatc gcctccatcg agaaccccga acgccaactg gccctggcca tgctctgcat
     6121 ccgccgcaac ctgtccgtcc gccagctcga agtgctggcc agacagcaaa ccaataccgt
     6181 cacgaaggtt gccgagcggg ttccccttca gaaccgccac atagcagaag tagagcaagc
     6241 gctcgcgcgc gggctcggct tgccggttaa actccgtccg ggacggaaaa agaactcggg
     6301 acgcgttgtc ataacgtacc ggaatttgga tgagttcgac ctgatcgcga ataaaatcgg
     6361 cggccactgc aaactggagt aggacaatgc cgctccgact accgatcgcc gcggggcgtt
     6421 tctatcccag cgacgccccc gaatgtgttg cgctgttgaa cgaaatgcag gctagcgtcg
     6481 agccggtccc ggcggtaggc gccatcgcac cgcacgcggg ctggatttac agcggccgca
     6541 cggccatgcg cagcatcgtg tcactggcag ccaagtcccc cgagacggta attgttttcg
     6601 gtgcggttca cgtcctcaca cacgcggacg ctagcctctt cagcgaaggg acttggcaga
     6661 cgccgctggg tttagtggcc gtcgacagcg acctgggcgg gcttattagg cgcatcgacc
     6721 acgtgcaaga tgacccggaa gcacatcggt tcgagcactc gattgaagtc ctgcttccca
     6781 tgatccaaca cgttctgccg gaggcgcgta tcgtaccgtt gatggtcaag ccgggccggc
     6841 gcgctgagca gatcgggcgt gattgcgccc gcgcggccaa ggaatccggg cgacgcgttg
     6901 cgtttctggc gtcgactgac ttgacgcact acggtccggc cttcggtatc gagccggtcg
     6961 gacggggcgc agcgggcgtg aaatgggcca aagaggtcaa tgatcgccgg ctgatcaagc
     7021 tgatagcggt gggacgtgcg ctggaagtgc tggcggaggc ggagcagagt cgcagcgcct
     7081 gcggtgcggg ggcggtggcg gcgctattgg gcgcgatggg cgaattgggg gcggtggaat
     7141 ttcgggagct ggagcacacc agcagcgccg aggtggagtg ggcggcgggc ggcggggcgg
     7201 cggtcaattc ggtgggctac ctcgccggcg tgtttttgta gaactgctag actccatatt
     7261 ctagaatgac tagacagggt tggcgcggcg gcggccggcc gttcggcgga cctcttccta
     7321 caataactag aaactgggga cggcaagctg ggccgggcgc tttatggaca tgcgcagcag
     7381 ccgcacgcgc cgctggtaag gcggcaagta gtgcacctcc caaccgtcct cgcgcaggcc
     7441 cgcccggtaa tagcaggcgc gggccggggc gttgtcggga aaaaccacga gcgtgacggc
     7501 cgcggccgcg tagttcgaaa aggcacggtt aagtaaggcg ctggtgagcg ccgcgcccac
     7561 gccgcaccgc cggcgctgcg gggccaccag cagatgtcct agccagtata gaccgcgctg
     7621 cgagtctagt acgttcaact caccgtaggc aatggggatg cgaccgcggg
//
LOCUS       DAOSYS010000178         7577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8262_length_7577_cov_5.405078, whole genome shotgun sequence.
ACCESSION   DAOSYS010000178 DAOSYS010000000
VERSION     DAOSYS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8262_length_7577_cov_5.405078"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLU99_11155"
     CDS             <1..365
                     /locus_tag="PLU99_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17653.1"
                     /translation="AVAYRVVVGYFPRAGQPQYLGVEFAIHSALLVLLAWLVPFLLDR
                     LLRPSLEAAVLSALRRGFDDGLAIVGDSLARALSRAGSEARQFREEGQTLIQQANTLT
                     TAPSAAREPVLQKVIART"
     gene            408..1271
                     /gene="mutM"
                     /locus_tag="PLU99_11160"
     CDS             408..1271
                     /gene="mutM"
                     /locus_tag="PLU99_11160"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOO17654.1"
                     /translation="MPELPEVETIVRRFRPLLEGRRITGFDTRWKKQLSPSLAAVRAG
                     CVGRRIVRLARRGKFIVFQLDPPGCLLVHLRMSGRFEWGANCECEPGHLRAIWTFEDS
                     ERLLFCDPRKFGRIIYAPDLATATGELGVEPLSRAFTVAKLAALLRRHRRRLKPLLLD
                     QRVIAGLGNIYTDEALFRAGLHPLTASDTLGPPQIAALQAAIRAVLREAIRYQGTTFD
                     QVYAGGDMQRRLAVYGRKDQPCPRCGAAIRYARIGQRGTHFCPRCQPVGGGRRKLTRR
                     AKRQTQAGAAR"
     gene            1306..3234
                     /gene="asnB"
                     /locus_tag="PLU99_11165"
     CDS             1306..3234
                     /gene="asnB"
                     /locus_tag="PLU99_11165"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOO17655.1"
                     /translation="MCGIAGILSLSPEARVENAALRTMADQLRHRGPDDAGFYLDPQA
                     RCGFAFRRLSIIDLKTGNQPLSNEDGTIWTVLNGEIYNYRELRAELVSLGHKFATQSD
                     TETIVHLYEQHGRACFERLSGMFAIAIWDQSRGRLLLARDRLGKKPLVYAVCGERLYF
                     ASEAKALLALPEMPRRLDPQSLHRYLIFQYVPAPHSIYTGFKKVLPGHYELFEAGAAQ
                     TPAWQEPRPRNCCGQAFWRVPDPPPFDGSYEDAKARLGELLTDAVAARLISDVPLGAF
                     LSGGIDSSIVVGLMRKLGVSPLRTFSIGFADARYDETPYARRIAQLFATEHHEYTVTP
                     QAREVLDTLAWHYDEPFADSSAIPTYYLSRWTRESVTVALTGDAGDECFAGYDRYRAA
                     QLAGSLDRLPHGMRRGLAKLAAIVPHGQPKTRTRQLYRFLRAVSQAPALRYMDWVNIF
                     SPAMLLAGYRPEFRAQLDLDEPVRWFDGLYNHTAGPPAARAIRTDFETYLPYDLLTKV
                     DIASMACSLECRSPFLDHRLVEFALSLPLSWRLRGRNTKRILKDWAAGLLPPEIMARR
                     KMGFAVPIGQWFRGELREYLRDLLFAPEALSGRIFRPEWLRDLFEGHLSGRANYEAPL
                     WSLLMVELWFRRWQPVVP"
     gene            3354..3761
                     /locus_tag="PLU99_11170"
     CDS             3354..3761
                     /locus_tag="PLU99_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HOO17656.1"
                     /translation="MLVDQAEIDALLAQATGGEAEGGGWVPEPPAPPPPAQRVAFTNL
                     NADVRRILRLRVPVIVQLARRRMMIAAIRKLAPGAIIEFDVSVAHSHDLLINNRPIGR
                     GKCVKIGEHFGLQITEVLDRAQRIRSMGSGPLE"
     gene            4126..5754
                     /locus_tag="PLU99_11175"
     CDS             4126..5754
                     /locus_tag="PLU99_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOO17657.1"
                     /translation="MKTELLLGDDAVALGAIHAGIGGAFSYPGTPATEIFEYIQAKAA
                     KLGGISARWSANEKVAYEEALGMSYAGKRAIVSMKHVGLNVAADPFVNSGLTGVNGGL
                     VLVVADDPGMHSSQNEQDSRYYGEFAQIPVIEPGNQQQCYDLTKQAFELSEEVRLPVM
                     IRLVTRLAHSRSNVRVDMPEGVPERARTRLPLPNPNDWTLVPAHARRRFRRLLDMQPA
                     LREWADRAPFNSLRLAGSTGIIASGIAYNYAREAIGADENYSLLRVSTYPLPMKLLRE
                     LVGHCNEIWVIEEGYPFIESRLNGLLGLPGKFVRGKLSGDLPATGELTPEIVARALGR
                     PCTAPIAAMEDLVARPPALCRGCPHADTFKAIIDATASYPESLLFSDIGCYTLGAMPP
                     YKAVHSCVDMGASIAMAHGASQAGAHPVICTIGDSTFTHSGMTPLIGAARANANMTVF
                     ILDNATVAMTGAQETMATGDDLLKLLAGLGVDPGHMKVIEPLSKNHAENVATIKGEIE
                     HRGLSVIVPQRACLFAMRKTVAAKSKPAAAPASA"
     gene            5812..6369
                     /locus_tag="PLU99_11180"
                     /pseudo
     CDS             5812..6369
                     /locus_tag="PLU99_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
     gene            6616..>7577
                     /locus_tag="PLU99_11185"
     CDS             6616..>7577
                     /locus_tag="PLU99_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOO17658.1"
                     /translation="MELVGAIQPPKYVFVPKGGMITPEVLAQYPGETVVLKIVSPQIT
                     HKTEAAGVVFVPKDFVAVKREIERMVTAHGANVEVEGVLVVEFVERRQAGFGHELFVG
                     VRATREFGPVIAAGLGGVDTEYLASKMRPGIAVAKACAMDITAEEFLELFKHTAAYDV
                     LSGRARGHGRIVSDGELLRCFRAFISIARHFCVDRGVEGPDIAELEVNPFAFRQQRMV
                     PLDGRGRLAPAAKARPARSIEKVRRMLEPRAAAVLGVSSSSMNFGRIILNNMKSCGFP
                     PEHLYIVKEEEKAIDGVPCVPNVAALPEPVDLLVIAAPAAAMPKV"
BASE COUNT         1255 a         2414 c         2537 g         1371 t
ORIGIN      
        1 ctgccgtggc gtatcgcgtc gtggtcggct atttcccgcg cgccggccag ccgcagtacc
       61 tgggcgttga gttcgccatt catagtgcat tgttggttct actcgcctgg ctggtgccat
      121 tcctgctcga ccgcctgctg cgtccgtcgt tggaagccgc tgttctgtcc gcgctgcgcc
      181 ggggatttga cgacggtctg gcgattgtcg gagacagcct cgcacgcgct ctgtcgcgcg
      241 ccggcagcga ggcccgccaa ttccgcgagg aaggccagac gctcatccag caggccaaca
      301 cgctcaccac agcaccctcc gccgcgcgcg aaccagtgct gcaaaaggta atcgcacgca
      361 cgtagcaccc gttgcccggc ggccgcgccg gctcgtagaa tcccggcgtg cctgaacttc
      421 ccgaagtcga aaccattgtc cgccgctttc ggccgctgct cgaagggcgg cgcatcaccg
      481 ggttcgatac ccgctggaag aagcagcttt cgcccagcct cgccgccgtt cgcgcgggct
      541 gcgttgggcg tcgcatcgtg cggcttgccc gccggggaaa attcatcgtc ttccaactcg
      601 atccgccggg ctgtctgctc gtacacctac ggatgagcgg gcgcttcgaa tggggcgcta
      661 actgcgagtg cgagccgggc cacctgcggg cgatttggac gttcgaagac tcagagcggc
      721 tgctgttctg cgatccacgc aagttcggac gcatcatata cgcgccggat ctcgccaccg
      781 cgaccggcga actgggcgtc gagccgctca gccgcgcctt caccgtcgcc aagctggccg
      841 ccttgctgcg ccggcatcgc cgccgcctca agccgctgct gctcgaccag cgcgtcatcg
      901 ccggcctggg caacatctac accgacgaag ccctctttcg cgccggcctg cacccgctga
      961 ccgcctccga cacgcttggt ccgccgcaga tcgcggccct gcaggccgcc atccgcgccg
     1021 tgctgcgcga ggccatccgc taccagggca ccaccttcga ccaggtctac gccggtggcg
     1081 acatgcagcg ccggctggcc gtctacggcc gcaaggatca gccctgtccg cgctgcggcg
     1141 cggcgatccg ctacgcgcgc atcggccagc gtggtacgca cttctgcccg cgctgccagc
     1201 cggttggcgg cggacggcgc aagctgacgc ggcgcgcgaa gcggcaaacg caagccggcg
     1261 cagcgcggta gcaccgccgc ccgactcgtc ttatcgtgag acgccatgtg tggaatcgcc
     1321 ggcatcctga gcctgtcgcc cgaggcgcgc gttgagaacg cggcgctgcg caccatggcc
     1381 gatcagcttc ggcatcgcgg cccggacgac gccggctttt acctcgatcc gcaggcccgc
     1441 tgcggcttcg cgttccgccg cctgtcgatc atcgatctca agaccggcaa ccagccgttg
     1501 agcaacgagg acggcaccat ctggaccgtg ctcaacggcg agatttacaa ctaccgcgag
     1561 ttgcgcgccg agcttgtgtc gctcggccac aagttcgcca cccaaagcga caccgaaacg
     1621 atcgtccact tgtacgagca gcatggccgg gcgtgcttcg agcgcctcag cggcatgttc
     1681 gccatcgcca tctgggacca gtcgcgcggc cggctgctgc tggcgcgcga tcgcttgggc
     1741 aagaagccgc tggtgtacgc cgtttgcggc gagcgcttgt attttgcgag cgaggccaaa
     1801 gcgctgctgg cgttgcccga gatgccgcgc cggctcgatc cgcagtcgtt gcatcgttac
     1861 ctgattttcc aatacgtccc cgcaccgcat tccatttaca ccggcttcaa gaaggtcctg
     1921 cccggccact acgagttgtt cgaagctggt gcggcgcaaa caccggcgtg gcaggagcct
     1981 cgccctcgca actgctgcgg tcaggccttc tggcgcgtgc cggatccgcc tccttttgac
     2041 ggcagttacg aagatgccaa ggctcggctg ggagagttgt tgacggatgc cgtggccgca
     2101 cggctgatct cggacgttcc gctaggcgcg tttctatcgg gcggtattga ctcgtcgatt
     2161 gtcgtcggtc tgatgcggaa gctgggtgtt agtccgctgc gcaccttcag catcgggttt
     2221 gctgatgcgc gctacgatga gacgccgtac gcgcggcgca tcgcgcagct ctttgccacc
     2281 gaacatcacg agtacacggt tacgccgcag gcccgggaag tactggacac gctggcgtgg
     2341 cactacgacg agccattcgc ggattcctcg gccattccca catactactt gtcacgctgg
     2401 acgcgcgagt cggtgacggt ggcgctgacc ggcgacgccg gggacgagtg tttcgccggc
     2461 tacgaccgct accgcgccgc gcagctcgcc ggcagcctcg accgcctgcc gcacggcatg
     2521 cggcgcgggc tggccaagct ggccgccatc gtcccgcatg gccagccaaa gacgcgcaca
     2581 cgccagctct accgttttct ccgcgctgtt tcccaggcgc cggctcttcg ttacatggat
     2641 tgggtcaaca tcttctcacc cgcgatgttg cttgcgggct atcgacccga gtttcgcgcc
     2701 caactcgacc ttgacgagcc ggtccgttgg tttgacgggc tgtacaacca caccgcaggt
     2761 ccgccggccg cgcgcgcgat tcgcacggat ttcgagacct acctgccgta cgacctgctc
     2821 acaaaggtcg acatagcgtc gatggcgtgc agcctcgaat gccgttcgcc gttcctcgat
     2881 catcgcctgg tcgagttcgc tctgtcgctg ccgctatcct ggcggctgcg tgggcgcaac
     2941 accaagcgca tcctcaagga ctgggctgcc ggactccttc cgccggagat aatggcccgt
     3001 cgcaaaatgg gctttgcggt gccgatcggc cagtggtttc ggggcgagct gcgcgagtac
     3061 ctgcgagatt tgttgttcgc ccccgaggcc ctttcgggcc gcatcttccg gccggaatgg
     3121 ctgcgagact tgttcgaggg gcatttatcg ggccgagcca attacgaggc gcccctgtgg
     3181 tcgttgctca tggtcgaact atggttccgc cgctggcagc cggtcgttcc ataggcgttc
     3241 gttgcaattc tggggcggca cggctctgcc cagttttcag ccgtttctgc cgttccgccg
     3301 atgtatttag ggcagccgct tatgggtctg gcggcggctg gagacgctgc gcgatgctgg
     3361 ttgatcaggc ggagattgac gcactgctag cccaggccac gggcggtgag gccgaaggcg
     3421 gcggctgggt gccggagccg ccggcgccac cccctccagc gcaacgggtc gcgtttacga
     3481 atctgaatgc ggacgtgcgc cgcattctgc ggcttcgggt gccggtgatc gtgcaattag
     3541 cgcggcgccg gatgatgatc gcggcgattc ggaagctggc gccgggtgcg attatcgaat
     3601 tcgacgtgtc agtggcgcat tcgcacgacc tgctgatcaa taaccgccca atcgggcgcg
     3661 gcaagtgcgt caaaatcggc gagcacttcg gtctgcaaat cacggaagtc ctggatcgcg
     3721 cgcagcgtat ccgttcgatg ggctctgggc ccctggagtg atcgaactcg aggtgggcgg
     3781 catagcgggg ggacattgtc cgactctgtt ctgttttggg aatccactgg gaaaaaacgc
     3841 gcacttcgcg ggtcgtatca tcctcaaggc gcgtcaagat ctccaatttg ggtaatcttg
     3901 cgcacgggcg ggatacgtga ggaactgggc aacggagcgg gcatctcagt agccaggtgt
     3961 tcttaagtcg tgcttcacga gcggcttacg gaaaaaaaag gcagcactgg gttctgttcc
     4021 gccgcgggaa tgccgcgtgc taagcggcgg acggtcagcg cgccgccggg agcctcggtt
     4081 cgctttagca agtcccggat caagcgccgg cccggaggat tgcagatgaa aaccgaattg
     4141 ctgctcggcg acgatgcggt cgctctcggg gcgatacatg ccggcatcgg cggcgccttt
     4201 tcttacccgg gcaccccggc tacagaaatt ttcgagtaca tccaggctaa agccgcgaaa
     4261 ctgggcggca tttccgctcg ttggtcggcc aacgagaagg tcgcctacga ggaagccctc
     4321 gggatgtctt acgccggcaa acgtgccatc gtctcgatga agcacgtcgg cctgaatgtg
     4381 gccgccgatc cgttcgtgaa ctccgggctg accggcgtga acggcgggct ggtgctggtc
     4441 gtggcggacg atcccggcat gcactcttcg cagaatgagc aggacagccg gtactacggc
     4501 gaattcgctc agattcccgt tatcgagccg ggtaatcagc agcaatgcta tgacctgacg
     4561 aagcaggctt tcgagctgtc ggaggaggtt cgcctgccgg tgatgatccg gctggtgacg
     4621 cgcctggcgc acagccgatc caacgtgcgc gtcgatatgc cggaaggcgt gccggagcgt
     4681 gcccgaacgc ggctgccgct gccgaatccg aatgactgga cgctggtccc ggcacacgcc
     4741 cggcggcgct tccggcggct attggacatg cagccggccc tgcgggagtg ggcggaccgc
     4801 gccccgttca attcgctgcg cctggcgggg tcgacgggga tcatcgcctc gggcatcgct
     4861 tacaactatg cacgcgaggc catcggggcg gacgagaact attcgctgct gcgggtatcg
     4921 acgtacccgc tgccgatgaa gctgctccgc gaactggtcg ggcactgcaa cgaaatctgg
     4981 gtcatcgagg aaggttatcc gttcatcgag agccgcctga acgggctgct cgggctgccc
     5041 ggcaagttcg tgcgcggcaa gctcagcgga gacctgcctg cgaccggtga gcttacgccg
     5101 gaaatcgtgg cccgggcgct ggggcggccg tgcaccgcgc cgattgcagc catggaagac
     5161 ctggtggcca ggccgccggc gctgtgccgc ggttgcccgc acgcggatac gttcaaagcc
     5221 atcattgacg cgacggcttc gtacccggag tcgctgctat tcagcgacat cggctgctat
     5281 acgctgggag cgatgccgcc atacaaggcg gttcattcgt gcgtggacat gggcgcctcg
     5341 atcgcgatgg cacacggggc gtcgcaggcc ggcgcccacc cggtcatctg tacgattggt
     5401 gattcgacct ttacacactc gggcatgacg ccgctgatcg gcgcggcccg ggcgaacgcg
     5461 aacatgacgg tctttatcct ggacaacgcc acagtcgcca tgaccggggc ccaggagacg
     5521 atggccaccg gggacgacct tctgaagctg ttggcggggc tgggcgtcga tcccgggcat
     5581 atgaaggtga tcgaaccgct ttcaaagaac catgccgaga acgtcgcgac gattaaaggc
     5641 gagatcgagc atcgcgggtt gtcggtcatc gtgccgcagc gggcgtgcct gttcgccatg
     5701 cggaagacag tggcggccaa gagcaagccg gccgctgccc ccgcgagtgc gtaggatagg
     5761 gacgagaata ttcctgcagc gccgtggtgc gctcacgaca gaggatcaat cgtggaacaa
     5821 aatatcattc ttgcgggcgt cggcggacag ggcattctca cgattgcgcg ggccatctcg
     5881 attgcagcgc tgcggcgcgg gctcacgctc aagcaggccg aggttcacgg catgtcgcag
     5941 cgcggcgggg cggtagagtc gcacctgcgc gtggcggacc atgagctgtt cagcgacttg
     6001 atcccgctgg gccaggccaa catggtgctc tcggtcgagc cgctcgaaac gctgcgctac
     6061 gtgcagtacc tgaacaacga tggcgtggtc gtggcgggca cgacggccgt agtgaatatc
     6121 gacaattatc ccgcgattga gcaggtgctc agccacgtca cgcgttttcc gcggcacgtg
     6181 ctgctggatg ctgaaggtct ggcgaagaag gctggctcgg cccggtcggc gaacatcgtc
     6241 tcgctcgggg cggcctcgat ttacctcgaa atcgacgccc aggacctgga ggacgcggtg
     6301 gccgagatgt tcgcgagcaa gggcgaggag atcgtacaga caaaccggcg ggcgttccgc
     6361 atcgggcgca acgcggctga cgcgtatcgg gccggcctgg gcgccgggct gacttcgcgc
     6421 gcggtgcgtg agcgaatcga cgcgctaagc cctgaagtgc tgctgagctc cgaggcgggc
     6481 gcggcggtct gcggcaacgg gcaggcgcgc gtcgatcggc tgaccggcgc ggaggcgcag
     6541 gcctttgaag ccacattgct acgcgtgtac gaggagggcc ggcggagctt ctacgagcac
     6601 gaagtctaca agctggtgga gctggtcggc gcgattcagc cgccgaaata cgttttcgtg
     6661 cccaagggcg gcatgattac gcccgaagtg ctggcgcaat atcccggcga aaccgtggtg
     6721 ctgaagattg tctcgccaca gattacgcac aagactgagg ccgccggtgt ggtgttcgtc
     6781 cccaaagact tcgtggccgt caagcgcgag atcgaacgga tggtcactgc ccacggggcc
     6841 aacgtggaag tcgagggcgt gctggtggtc gagttcgtcg agcgccgcca ggccggcttc
     6901 gggcacgagc tgttcgtggg cgtgcgagcc acacgcgagt ttgggcccgt gatcgcggcc
     6961 ggcctcggtg gagtggatac cgagtacctg gccagcaaaa tgcgcccggg gatcgccgtc
     7021 gccaaagcct gcgcgatgga tattacggct gaggagttcc tggagctgtt caagcacacg
     7081 gcggcatacg acgtgctctc gggccgggcc cgcgggcacg ggcggattgt ctccgatggc
     7141 gaactgctcc gctgtttccg ggcctttatc tcgatcgcac ggcatttctg cgtcgatcgc
     7201 ggagtggagg ggccggacat cgccgaactc gaggtcaacc cgttcgcgtt ccgccagcag
     7261 cgcatggttc cgctcgacgg ccgcggacgt ctggcgccgg cggccaaggc tcgcccggcc
     7321 cggtcgatcg agaaggtgcg gcggatgctc gagccgcggg cggcagccgt gctgggcgtc
     7381 tccagctcat ccatgaactt cggccggata atcctgaaca acatgaagag ctgcgggttc
     7441 ccgccggagc atctgtacat cgtaaaggag gaagagaagg cgattgacgg cgtgccgtgc
     7501 gtgccgaacg tggcggcact gcccgagccg gtggacctgc tggtaatcgc cgctccggcg
     7561 gccgcaatgc cgaaggt
//
LOCUS       DAOSYS010000179         7510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8356_length_7510_cov_4.696579, whole genome shotgun sequence.
ACCESSION   DAOSYS010000179 DAOSYS010000000
VERSION     DAOSYS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8356_length_7510_cov_4.696579"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1241
                     /locus_tag="PLU99_11190"
     CDS             93..1241
                     /locus_tag="PLU99_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17659.1"
                     /translation="MTLRLTAIGLLAALGFSGLILASNFECAKATHDPVPSRPAATAT
                     GVRYRGIAVQAQNSYRPIEFYGQIFRDISNLGANTVLLCAAGFMEHAEAQQIFLEARK
                     MPSREEFKTIIGMAKKEGLEVILMPIVLLSKPRGSEWRGVIKPPDWDEWWDDYREFIK
                     YFCEIAREGGAHGLMVGSELVSTEKFTEKWNGVIELAREHFHGGKLGYSANWDHYKPI
                     RFWHKLDFIGMTSYYTLADKKDPSVEEIVERWQPIREEVLGWQREIGKPLIFTEVGWC
                     SQEGAATAPWNYFQNQKATPAGHEEQRRLYEAFLKAWDNAPGVAGVIWWDWSGDPGGP
                     DDFGYTPRGKPAEDVLRDWFAKYNGRDGAEEAQVAEKPGEGTPKAAAP"
     gene            1504..2784
                     /gene="ribA"
                     /locus_tag="PLU99_11195"
     CDS             1504..2784
                     /gene="ribA"
                     /locus_tag="PLU99_11195"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOO17660.1"
                     /translation="MMESTGPTADAGTASPFSPIDEILDELRQGNLIILVDDEDRENE
                     GDLVCPAQSITPEKVNFMLRYGRGTLCVPLTRARCERLNLHPQVPVNNTRLGTAFTVT
                     IDAASHLGVTTGVSAWDRATTIRHTVAEEAVAEDFVRPGHVNPLAACDGGVLVRAGQT
                     EGSVDLCRLAGLLPGAAIIEVLNEDGTMARLPQLTEFSRRHGIKMCTVAALIEYRMRR
                     ERLVERIETVPLYNEFGEWLLIGYRSQTDKYEHVALCKGGIGELDETGAVRAIEEPVL
                     ARVHSECLTGDVFGSMRCECGEQLRAAAQLIAKQGRGVIVYLRQEGRGIGLLNKLHAY
                     RLQDGGLDTVEANQQLGFPADRRDYGVGAQILRDLGIEKVRILTNNPKKVGRLNVYGL
                     EIVEQLPLEIPPTAHNRRYLEAKKTKMGHTLREV"
     gene            complement(2794..>3276)
                     /locus_tag="PLU99_11200"
     CDS             complement(2794..>3276)
                     /locus_tag="PLU99_11200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOO17661.1"
                     /translation="PAVQDFYPDDLAHCYGCGRLNEHGLHVRSSWDGDETVAILTPRP
                     YHTAVPGYVYGGLIASLIDCHGTGSAALAAYRAAGREPGTLPPLRFVTASLKVDYLRP
                     TPMGVPLEARGRIVEVKPRKVIVDVTVSANGEVCAKGNVVGVLMPESMLPREASAQPE
                     "
     assembly_gap    3277..3376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3377..3669)
                     /locus_tag="PLU99_11205"
     CDS             complement(<3377..3669)
                     /locus_tag="PLU99_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOO17662.1"
                     /translation="MMEGKVEEFFEQWSEDQAKLKELAPDIQRGFGALFQATMKEGAL
                     SVREKELIALGIALAVRCVPCINLHVEKCITAGATPQQVLEAAGVAVTMQGGPA"
     gene            complement(3864..5003)
                     /locus_tag="PLU99_11210"
     CDS             complement(3864..5003)
                     /locus_tag="PLU99_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOO17663.1"
                     /translation="MRKFRLFTPGPCAVPEEVLQEMARPFHHRTDWFKGYMKQATEKL
                     QQVLQTKYEVIIITGSGTAAAEAAIVGCNPPGSKMLTLEGGKFGQRWGEIAQQFGIDV
                     IRHPVEWGTPPTAAMVSDFLKKDPAITAVMVVHSETSTATACDLKAIAGVVRDAGKLM
                     LADCITSAGALPLKPDEWGVDVVITGAQKSLMLPPGLGFLAVSPRAWEVIERNKAQHA
                     YYLNLNKARKMAKENDTPFTPAHLLIRGLNTALDIVLADGMETMWKRVAAMAAATRAA
                     GEAIGLKVFSKSPSDSVTALCPPEGIAVKDIRNELEKRYGIESAGGQDQLKGKIFRLG
                     HMGYVDEMDTILAIGALEQVLYKAGHKFNLGAGLTAAQKILAERL"
     gene            complement(5394..6122)
                     /locus_tag="PLU99_11215"
     CDS             complement(5394..6122)
                     /locus_tag="PLU99_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HOO17664.1"
                     /translation="MTTSAPDRIERSLSDKGVGGEGGFLDQILGASQSPTRQGGGAAT
                     GLKEMISSIFSDPRALRAGGLGALAGAIFGRGAKGAVGGGALALLGSLALKALQGAQG
                     GKKPQLDPSTQLEAGLRPPANHQEQERVESQAELLVAAMANAAKADGQIDQREIEHIT
                     GKVQADEASRRRLMEELRQPMDTERIVRAVNNNPQLAAQVYAASLLAINVDTEAERRY
                     LADLAAKLRLDPAVVQNLHDALGV"
     gene            complement(6582..>7510)
                     /locus_tag="PLU99_11220"
     CDS             complement(6582..>7510)
                     /locus_tag="PLU99_11220"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOO17665.1"
                     /translation="PANACLLADPQKTALNVFSTGEGVHVLLPPEAPDPSNSVVVLDI
                     VGRLDIAVPPVISAPTHIFIDTLDVTITSDRQNVELHYTTDGNCPTPASPVVQGPLKL
                     SETTMISARAFRDDLPVSSTVQRTFIKVAPRPAEPAEKIAGASPGLLCEYFEGEWDRL
                     PDFDKLEPVRSASVTNLGFFMDAATSPVAPEQSPTAVESDFAAAIRPEYVALRYRGFV
                     AVPRDGIYTFETRSDDGSRLYIGDQCVVNNDGLHGAVTESGLIALSAGAHPLTLTYFN
                     KTGPRALEVWYAGPELDRQELPSAALNHLVIK"
BASE COUNT         1308 a         2350 c         2395 g         1357 t
ORIGIN      
        1 agtcgacacc gcccggaggc ctgccacagc cgtccaaagc acgtgaattc cggcatgaca
       61 ggtgggccgg gcgaagataa tcttacccaa ccatgacgct acgtctgaca gcaattggtc
      121 tcctcgcagc ccttggcttc agcggcttaa tcctggccag caatttcgag tgcgccaaag
      181 ccacgcacga tcccgtgccg tcccgcccgg ccgcaaccgc caccggcgta cgatatcgcg
      241 gcatcgcggt gcaggcccag aacagttacc ggccgatcga gttttacggc cagatttttc
      301 gcgacatctc caatctcggg gcgaacacgg tcctgctctg cgcggccggc tttatggagc
      361 acgccgaagc ccagcagatt ttccttgaag cgcgcaagat gccctcgcgc gaggagttca
      421 agaccatcat cgggatggcc aaaaaagaag gcttggaagt catcctgatg ccgatcgtgc
      481 tgctctcgaa gccgcgcggc agcgaatggc gcggcgtcat caagccgccc gattgggacg
      541 aatggtggga cgactaccgc gagttcatca agtacttctg cgagatcgca cgcgagggcg
      601 gcgcccacgg cctgatggtc ggctcggaac tggttagcac ggaaaaattc accgagaagt
      661 ggaacggcgt aatcgaactg gcacgcgagc acttccacgg cggcaagctg ggctattcgg
      721 ccaattggga ccactacaag ccgatcaggt tctggcacaa gctcgatttc atcggcatga
      781 ccagctacta cacgctggcc gataagaaag accccagcgt ggaggagatc gtcgagcgct
      841 ggcagccaat ccgtgaggaa gtgctcggct ggcagcgaga gatcggcaag ccgctgatct
      901 tcaccgaggt gggttggtgc agccaggaag gcgccgccac cgccccgtgg aactacttcc
      961 agaatcagaa ggccactccc gccgggcacg aagagcagcg ccgcctttac gaggccttcc
     1021 tcaaggcttg ggacaatgcg cccggcgtgg cgggcgtgat ctggtgggac tggtccggcg
     1081 acccgggcgg cccggacgat ttcggctaca ccccacgcgg caagccggcc gaagacgttc
     1141 tgcgcgactg gttcgccaag tacaacggcc gggacggagc cgaagaggcg caggtggccg
     1201 aaaaacctgg cgaaggcacg cccaaggcgg cggcgccgta atcgccattg gataatcaag
     1261 ctctcgttta tgggctgcga gccggcgttc cgtgagctca gccgtctttc agcattctga
     1321 gtgcagcgtt cggcattccg cgtgcggcgc caacggcgtt tcatctgcat tcagcatgtt
     1381 gcatttggca cttggcgttt gcgctacccc ccgctgcggc cagaccaccc ctgccgtcct
     1441 ggcgcgccgt ttgctacaat cccctcttac agccttacag cgatagcagc gaaggacgtg
     1501 cgcatgatgg aaagcaccgg gcccacggcc gatgcgggca cagccagccc attcagcccg
     1561 attgacgaaa ttctcgacga gctgcgacag ggcaacctca tcatcctcgt cgatgacgaa
     1621 gaccgcgaaa acgagggcga cctggtttgc ccggcacagt ccataacgcc cgaaaaagtg
     1681 aatttcatgc tccgctacgg tcgcggaacg ctctgcgtgc cgctgacgcg cgcccgctgc
     1741 gagcggctga atctgcatcc gcaggtgccg gtgaacaaca ctcggctggg gaccgcattt
     1801 acggtgacga tcgacgccgc ctcgcacctg ggcgtcacga ccggcgtgtc cgcctgggac
     1861 cgcgccacca ccatccgtca caccgtggcg gaagaggcgg tcgctgagga tttcgtccgc
     1921 ccggggcacg tgaacccgct ggcggcctgc gacggcggcg tgctggtgcg cgccgggcag
     1981 accgaaggct cggtcgatct gtgccgcctg gccggactgc taccgggcgc cgcaatcatc
     2041 gaagtcctca acgaagacgg cacgatggcc cgcctgccgc agctgacgga attcagccgg
     2101 cgccacggca tcaagatgtg caccgtcgcg gcgttgatcg agtaccgcat gcgccgcgag
     2161 cgcctggtcg aacgcattga gaccgtcccg ctgtacaacg aattcggcga gtggctgcta
     2221 atcggctatc gcagccagac cgacaagtac gagcacgtcg ctttgtgcaa aggcggcatc
     2281 ggcgagctgg atgaaaccgg cgccgtgcgc gcgattgaag agccggtcct cgcccgcgtg
     2341 cattccgaat gcctgaccgg cgacgtcttc ggctcgatgc gctgcgagtg cggcgagcag
     2401 ctccgagccg cggcccaact gatcgccaag caaggccgcg gcgtgatcgt ctacctgcgc
     2461 caggaaggcc gcggaatcgg actgctgaat aagctgcacg cctaccggct ccaggacggc
     2521 ggcctcgata cggtcgaggc gaaccagcaa ctgggctttc cggccgaccg ccgcgattac
     2581 ggtgtcggcg cccaaatcct acgcgacttg ggcatcgaga aggtccgcat tttgacgaac
     2641 aaccccaaga aagtcggccg gctgaatgtc tacggcttgg agatcgtcga gcagcttccg
     2701 ctcgagatcc cccctaccgc tcacaaccgc cgctacctcg aggcgaagaa gaccaagatg
     2761 gggcacacgc tgcgcgaggt gtagttcccg cttctactcc ggttgtgcgc tcgcttcgcg
     2821 cgggagcatg ctctcgggca tcagcacgcc aactacgttg ccttttgcgc agacttcgcc
     2881 attggccgac actgtgacgt ccacgatcac tttgcgcggc ttcacctcca cgattcgccc
     2941 gcgggcttcc agcggaacac ccatcggcgt cgggcgcagg tagtcgacct tcagtgaggc
     3001 cgtcacaaag cgcagcggcg gcaatgtgcc gggctcgcgc cccgcggcgc ggtaggccgc
     3061 cagggccgcg gaaccggtgc cgtgacagtc gatgagcgag gcgatcaaac cgccatagac
     3121 gtagccgggc acggcggtgt gataaggccg cggcgtaagg atcgcgacgg tttcgtcgcc
     3181 gtcccacgaa ctgcggacgt gcaggccatg ttcgttcaac cggccacagc cgtaacagtg
     3241 cgcgagatcg tcggggtaaa agtcctgaac cgccggnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnngcgg ggccgccctg catcgttacc gcaacgccgg ccgcttccag
     3421 cacctgttgc ggtgtggcgc cggccgtgat gcacttttcg acgtgcagat taatgcacgg
     3481 cacgcaacgc accgccagag cgatgccgag cgcgatcagc tccttttcgc gcaccgacag
     3541 cgcgccctct ttcatcgtgg cctgaaacag agcgccgaag ccgcgctgga tatccggcgc
     3601 cagttccttc aacttggcct gatcctccga ccactgctcg aagaactcct cgactttgcc
     3661 ttccatcatg gttcctttca gttggttgcc aggctctact ggtgattgtc aggctcgccg
     3721 tacgcgttct cgctctcagt aacgtaaccc aactcttaca gggcctcaac gaccgcgcac
     3781 aagcgatctc cgcgttgcgg atctcgcttg tgcgcgccgt tgtacgttca aatccggcca
     3841 gttgtggccg ttatcctggc aagttacagc cgttcggcca gtatcttctg ggctgccgtc
     3901 aggccggcgc ccaggttgaa cttgtggccg gccttgtaga gcacctgctc caaagcgccg
     3961 atcgccagaa tcgtgtccat ctcgtcgacg tagcccatgt ggccgaggcg gaagatcttg
     4021 cccttgagct ggtcctgacc gccggccgac tcgatgccgt aacgcttctc cagctcgttg
     4081 cggatgtcct tgaccgcgat gccctcgggc gggcagaggg ccgtaacgga atcggacggc
     4141 gacttggaga agaccttcag gccgatggcc tcgcccgcgg cgcgggtggc ggcggccatg
     4201 gcggcgacgc gcttccacat ggtttccatg ccgtcggcca ggacaatgtc cagggcggtg
     4261 ttgaggccgc ggatcagcag gtgggccggc gtgaagggcg tgtcgttctc cttggccatc
     4321 ttgcgggcct tgttgaggtt caggtagtag gcgtgctggg ccttgttgcg ctcgatgacc
     4381 tcccaggcgc gcgggctgac ggccaggaag cccaggcccg gcggcagcat gagcgacttc
     4441 tgggcgccgg tgatgaccac gtctacgccc cactcgtccg gcttgagcgg cagagcgccg
     4501 gccgacgtga tgcagtcggc cagcattaac ttgcccgcgt cgcggacgac gccggcgatg
     4561 gccttcaggt cgcaggcggt cgcggtggag gtttcgctgt gcacgaccat gacggccgtg
     4621 atggccgggt ccttcttcag gaagtcggaa accatcgcgg cggtgggcgg ggtgccccac
     4681 tcgaccgggt ggcgaatgac gtcgatgccg aactgctgcg cgatttcacc ccagcgctgc
     4741 ccgaacttgc cgccttcgag cgtcagcatc tttgagccgg gcgggttgca gccgacgata
     4801 gcggcctcag cggccgcggt gccggagccg gtgatgatga tgacctcgta cttggtctgg
     4861 aggacctgct gaagcttctc ggtggcctgc ttcatgtagc ccttgaacca gtcggttcgg
     4921 tgatggaatg ggcgggccat ctcctgcagg acttcctcgg gaacggcaca ggggccggga
     4981 gtgaaaagac gaaactttct catcgcagta cctcatttgt gggccgccgc agcggccggg
     5041 ccgaaatcga caaacccgga attgtacgtg ctggcacgtc gcgcgccgag cgaggcgggg
     5101 ccagggtccg atatctagag agctgtcggc tgtcagctct cagctttcag ccagagtgcg
     5161 cgggttgtgg gttcctgcgc gcgggtggcg gtgccttcgg gctgcgccgg tggggagggg
     5221 tgataaggtg ataaagtgat agggtaataa gggggcgcgc gacacggccg gagcgccggc
     5281 tgggcgcgcg cacgggtggg ccggccgaca gggtgatagg gcgataaatg tgaactgcgg
     5341 ccctatcacc ttcagtgtgt cttctcgatc acacgccttg tatgtcttct cgctcagacg
     5401 ccgagcgcgt cgtgtagatt ttgcaccacg gccgggtcga ggcgtagctt ggcggccagg
     5461 tcggccaggt aacgtcgctc cgcctctgtg tccacgttga tcgccagtag tgaggccgcg
     5521 tagacctggg cggccagttg ggggttgttg ttcaccgcgc ggacgatgcg ctcggtatcc
     5581 atcggctggc gaagctcttc catgagccgc ctgcgggagg cttcatccgc ttgcaccttg
     5641 ccggtgatgt gctcgatttc gcgctgatcg atctggccgt ccgccttggc ggcgttggcc
     5701 atcgcggcca ccaggagctc agcctgcgac tcgacccgtt cttgctcctg atggttggcc
     5761 ggggggcgca aacccgcctc cagttgtgtc gatggatcga gctgcggttt tttcccgccc
     5821 tgggctccct gcaacgcctt gagcgccagc gaacccagca aggccagagc accgccgccc
     5881 accgcgccct tggcgccgcg gccgaatatg gcaccggcca gcgcccccag tccgcccgct
     5941 ctcagcgcgc gggggtcact gaatatggaa ctgatcattt ccttcaggcc cgtcgccgcg
     6001 cccccgccct gcctcgttgg cgactggcta gcgcccagga tctgatccag aaacccgcct
     6061 tctccgccga cccctttgtc actcagagaa cgctcgatgc gatccggggc ggaggttgtc
     6121 attccgccac ctagcagaga tccgagaatg tccaatgaac cggccatact ctcttcttcc
     6181 tttcgtctgc atcagcccgg aggcgcgcag ttgttgtttc gtgattgcta atcaacgtca
     6241 ccgctgttca accttattgg cagccgggaa ccagcgcggc agacgcttgc cgggcctcca
     6301 ggacgccggc gggtactccc gcggcctttc ccatactcgc ggctggtctt gccagactgg
     6361 catcaaagat tttttgcggc gacgccccgg ggacactgtc cacccaggga agagagacac
     6421 tagcgcctta tgctgaacag tctctgaatt ccgagtgacg gcgttgtaga cggggaccgg
     6481 gcgcggcatg aagctgtgat taggtgataa agtgataagg tgatagggtg ataaagggcg
     6541 gtccgcgaag ccccttatca ctctatcacc ttatcccttt atcactttat cacgaggtgg
     6601 ttcagcgcgg cgctgggaag ttcctggcgg tccagctctg ggccggcgta ccagacttca
     6661 agggcgcggg ggccggtctt gttgaagtac gtgagcgtga gcgggtgtgc gcctgcggac
     6721 agggcgatca ggcccgattc ggtcacagcg ccgtgcagac cgtcgttatt cactacacac
     6781 tgatcgccga tgtacagccg gctgccgtcg tctgacctgg tctcgaatgt atagatgccg
     6841 tcgcgcggca ctgccacgaa gccgcggtag cgtagggcca cgtactccgg gcgaattgcg
     6901 gcggcgaagt cggattcgac ggctgtcggc gactgttccg gcgcgacggg actcgtggcg
     6961 gcgtccatga agaaacccaa attcgttacg ctggccgacc gcaccggctc aagcttgtca
     7021 aaatcgggca ggcggtccca ctcgccttcg aaatactcgc agagcaggcc gggggacgcg
     7081 ccggcgatct tctccgccgg ttcggccggc cgaggcgcga ccttgatgaa ggtgcgctgc
     7141 actgtgctgc tgaccggcag gtcgtcgcgg aaggcgcggg cgctgatcat cgtggtttcc
     7201 gagagcttca gcgggccctg tacaacgggc gacgcgggcg tcgggcaatt gccgtcggtc
     7261 gtgtagtgca actcgacatt ctgccggtcg gacgtaatgg ttacatccag cgtgtcgatg
     7321 aaaatgtgag ttggcgcgga gattaccggc gggaccgcga tgtcgaggcg accaactatg
     7381 tccagcacaa ccacgctgtt gctcgggtcc ggcgcctccg gcgggagcag cacgtgcacg
     7441 ccttcaccag tgctgaagac gttgagcgcg gttttctgcg ggtcggccaa cagacacgca
     7501 ttcgcgggct
//
LOCUS       DAOSYS010000180         7507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8359_length_7507_cov_5.156065, whole genome shotgun sequence.
ACCESSION   DAOSYS010000180 DAOSYS010000000
VERSION     DAOSYS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8359_length_7507_cov_5.156065"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLU99_11225"
     CDS             complement(<1..1255)
                     /locus_tag="PLU99_11225"
                     /inference="COORDINATES: protein motif:HMM:NF021524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HOO17666.1"
                     /translation="MRVRLRRFWDNLNSGFWFFPAVLLAASALLAVLTLRLDENYFEG
                     RQPSIDFLWTGDADGARELLATVAASIITVAGVVFSILIVALALASQQFGPRLLRNFT
                     RDRGNQLSLGVFIGTFLYCLLVLRTVRHGPGETSFVPNLSVTTAIVLTTTSLGVLIYF
                     IHHVATAIQAENVVAAVGRDLDAAIRLYFPDSARTHRNETCDADGFDFSTAAPVPARR
                     TGYLQAIDTQALIRLAEQHDLLVRATRRPGHFITEGAPVAWVCPAAKCTPGLAQAVCD
                     TLMVGNQRTATHDIEYSIYQLVEVAVRSMSAGINDPFTAIACVDWLGAAIVQIAHRSF
                     PPACRYDGQGRLRLILNMVDFEGIVNAALDQIREVARDNVAVSVRILEVLATIAGQAP
                     TSEQRQILLKHARMVRAQCETAFPEE"
     gene            1544..1888
                     /locus_tag="PLU99_11230"
     CDS             1544..1888
                     /locus_tag="PLU99_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17667.1"
                     /translation="MLKFAVFKSVFTSKKAFGLALLASVCLVLSGCDKVTPENYSKIK
                     EGMTVSQVEDILGPGTENVGAAGAIGDLAGSVRVMTWGDDKKSITVTFVNDKVVAKVA
                     QGIGPPSPEAER"
     gene            complement(1935..5051)
                     /locus_tag="PLU99_11235"
     CDS             complement(1935..5051)
                     /locus_tag="PLU99_11235"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOO17668.1"
                     /translation="MRHTSRATTLNRLACVFISLAFSASAVAGPLRFVATPGPTFEYP
                     ADPPMRLPTAVSVAPDGVVYVADGVNDRILVFAPTGEIQEEIHTIEQQTLSRPISLKL
                     DAAGSLWIADSGNGRILVRAPNGALAREILISQPPGAPPPDITDLALSPDGRRLWLAD
                     NHNHHLLDVDLRTGLSRPLGRPGESLGQYFYPFMLATSATGDVLVTEPVTGRVQVHNA
                     EGQAVGTLGTFGVDLGQLYRPKGVAVDSAGNVWVADNIMGVVQIFEITGTLIDVLRDP
                     AGEPLKLEMPCGIALDADDNLYVAELTANRVRKFTIESNPKAPVRPAARPARPGRAAQ
                     PQACTRCHLEWLAPFASGQSTVLMSLPPVTEIPPAGQAEMCLSCHDGSVVDSRRRVWL
                     EHGHSTDVEPPPGMDVPAFLPLADGKIVCRTCHSAHVSGQPTGDIATAVFLRVPNLAS
                     ELCAACHIEKTRGREAGMHPTGGMPWPVPDVLLQAGARVGPNPRELTCQVCHTPHGAP
                     YERLLVMGTESNQLCLSCHEQMRPGMFREGRAEHPLSPVVNAEQAAAVREMGTQLGPD
                     GRLICLSCHRLHEAEGQRFLLAYDLAEGQMCLRCHEDKRVLLGSPHDLRQNFPQERNR
                     LGMTAHSGGPCSSCHLFHRYARAPEVSALDPGGGKCITCHQPGRVAHATVLSDVNHGH
                     VACAECHDPHTEKFTEFLRNTPALLCSSCHPEQSGLAGGPHDVTRNPAAWPEVAVATH
                     DVCLTCHRPHGNQETGLFRAGYARGQDRPDAACLACHLDAARGSESATALLHTRDANQ
                     IAGEMGLPLGADDGEKSIICRTCHNPHAATDFALLRVQSGVSSQQLCFHCHREMAHIR
                     DIGHGLPELQGAGFSVQVCGPCHVVHGRHASIEEPLLWPRDLTPAAEVLQAARPADRY
                     CLACHRAGGPVAPPAIATHPATEMYNPTAANAPSFLPLFNQQGEMDANGTIACRTCHL
                     THGRTHPIPAPAALGPLTPRELRAREWHLRSFGPESVCVYCHGADALRRFMYFHDAAR
                     RGGPLQKL"
     gene            5185..7038
                     /locus_tag="PLU99_11240"
     CDS             5185..7038
                     /locus_tag="PLU99_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOO17669.1"
                     /translation="MPDGQEQHAGPEPSSGSNQAGERPPDAGAKRPRWRRRWRKLAIA
                     AAVFVLLAALGMGGAEYYTSRPDFCASCHIMGPYYRSWSRDVHSQKVSVWCVECHYAP
                     GEQHTIHAKFKGLSQLASYFSGRYGASRPRAHVNDASCLTASCHGDREYLTTALLIGE
                     PRVEKRIIGGQESEVVRTPTVTFVHEKHLDVQSKLAENQAQLEQVRQRLRRAAKPEEY
                     EAIVTVARQVGPAPQWEKDLRGKFEQGLPELLADALELARLEHRQTRLRQLAGLNCAA
                     CHGYDGSGEHHLLPADLQTCFICHFNNQAFNTDTGECLKCHEPPTRKILVHDQAVTAA
                     WQREFPETTQPAGPALMDHREIVERGVNCASCHLDVIQGQATVTERDCSNCHDQERYL
                     AEFATRDTSTVEEYHRVHVKAQRARCVDCHHVIQHELIDPTHVATSAGFLRPILDDCQ
                     HCHPNHHHEQVELLMGIGGVGVGRPMPNAMFGSRLNCTGCHTRPALDMKGAELIAASE
                     QTCIDCHGSDYQRLFEQWRAEVAFSLGEAETALERVEKRIDELQSRGLEIPAEVADLL
                     APARRNILFVKAGNGIHNKNYALRLLDLSIRDLDDALGILTREGRPRFGQQ"
BASE COUNT         1373 a         2240 c         2573 g         1321 t
ORIGIN      
        1 gttcttcagg gaaggcggtt tcgcattgtg cgcggaccat tcgcgcgtgc ttcaagagta
       61 tttggcgctg ttcggaggtt ggcgcctgcc cggcgatagt ggccagaacc tccaagatgc
      121 gcacgctgac cgcgacgtta tcgcgggcga cctcgcgaat ctgatcgagg gcggcgttaa
      181 caatgccttc gaaatccacc atgttcagta tcagtcgcaa gcggccctga ccgtcatagc
      241 ggcaggcagg cggaaacgag cggtgggcga tttgcacgat tgcggcgccg agccaatcca
      301 cgcaggcgat cgcggtgaac ggatcgttga tgcctgccga catggagcgc acggcaacct
      361 ccaccagttg ataaatcgag tactctatgt cgtgcgtggc tgtccgctgg ttgccgacca
      421 tgagagtgtc gcagaccgcc tgagccagcc cgggtgtgca cttggcggcc gggcaaaccc
      481 aggcgactgg ggcgccttcg gtgatgaagt gaccgggccg gcgagtggcg cgcacgagca
      541 ggtcgtgctg ctcggccaac cggatgagcg cctgggtgtc aatggcctgc aaatagccgg
      601 tgcggcgagc gggcaccggg gcggcagtgg agaaatcgaa gccgtctgca tcgcaggttt
      661 catttcggtg tgttcgggcg ctgtcgggaa agtaaagccg gatggccgcg tccaagtcgc
      721 gtccgaccgc ggccacgacg ttttccgcct ggatggcggt cgccacgtgg tggatgaagt
      781 agatgagcac gccaagcgag gtggtggtca gcacaattgc ggtcgtgacg gagaggttcg
      841 gaacaaacga agtttcaccg gggccgtgac gcacggttcg cagcaccagg aggcagtaaa
      901 ggaacgtgcc gatgaaaaca ccgaggctga gctgattgcc acggtcgcgc gtgaagtttc
      961 gcagcaatcg gggtccgaac tgttggctgg ccagagcaag tgcaacaatc aaaattgaga
     1021 agaccacgcc cgccaccgtg atgatggaag ccgctactgt ggccagcagc tcccgcgcgc
     1081 cgtctgcatc accggtccac aggaaatcga tactgggctg cctgccttca aagtaatttt
     1141 cgtccagccg gagggtgagc accgccagca gcgccgaggc cgccagcagg acggcgggga
     1201 agaaccagaa gccgctgttc aggttgtccc agaatcttcg caggcgaacg cgcatggttc
     1261 cggtccaggc acgacggttt catctttcgg gcgagcgtgc cggggaatca aggccgacac
     1321 atccaacaga cgcagcacct ctaacagaat atgacccctt tctggcaggg atcgcgctta
     1381 agcagaagga gagagatcgt tactccggcg catgcccgcg cttacagctt gggcatggca
     1441 tccatgtttg cacatgctca ccgggtgtaa acgcccgata agcgcggtcg ccggggcgat
     1501 tctgacccgt atgatcaaat cggctgtgcc ttggagattg acagtgttga aatttgcagt
     1561 gttcaaatca gtgttcacat cgaagaaagc gttcggcttg gcgctgcttg catcagtctg
     1621 cctcgtcctt tccggctgcg acaaggtcac gccagagaat tacagcaaga tcaaagaggg
     1681 tatgacggtc agccaggtcg aggacatact cggcccgggt acggaaaacg ttggtgcggc
     1741 cggggcgatc ggtgacctgg cagggtccgt ccgcgtcatg acctggggcg acgataagaa
     1801 gtcgataact gtgacgttcg tgaatgacaa agtggtcgcc aaggtggcgc aaggcatcgg
     1861 accgccgtcg ccagaggccg agcgctgagc agcgaacctt tgaaggggcc gagcgctgag
     1921 cgagcgcccg gaagctacag tttttgaagc ggtccaccgc ggcgcgccgc atcgtggaaa
     1981 tacatgaagc gccgcaaggc atctgcgccg tggcagtata cgcaaacgct ctccggcccg
     2041 aaggaacgca gatgccattc gcgggcgcgc agctcgcgcg gtgtaagcgg gcctagcgcc
     2101 gccggcgccg ggatgggatg agtacgcccg tgcgtgaggt ggcaggtgcg gcaagcgatc
     2161 gttccattgg catccatttc gccctgttgg ttgaaaagcg gcaggaagga tggcgcattc
     2221 gcggcagtag ggttgtacat ttcggtcgcg ggatgcgtgg caatggcggg cggggcgacc
     2281 gggccacccg cgcggtgaca ggccagacag tatcggtcgg ccggacgcgc cgcctgcaag
     2341 acttcggccg ccggcgtcag gtccctaggc cataggagcg gctcttcaat cgaagcatgt
     2401 cggccatgca cgacgtggca gggtccacat acctggacgc tgaatcccgc accctgcaat
     2461 tctgggagcc catgcccgat gtcccgaatg tgggccatct cgcgatggca atggaaacag
     2521 agctgttgcg aactgacgcc ggactgcacg cgcaagaggg caaaatcggt ggccgcatgc
     2581 gggttatggc acgtgcggca gatgattgac ttctcgccat cgtcggcgcc cagcggcaag
     2641 cccatctcgc ctgcaatttg attcgcatca cgcgtatgta gcagggccgt cgcactttcg
     2701 ctgccgcggg ccgcatcgag gtgacacgcc agacacgccg cgtcaggccg atcctgcccg
     2761 cgcgcatagc ccgcgcgaaa caggccggtc tcctgattgc catgcgggcg atggcaggtg
     2821 aggcatacgt cgtgggtcgc gaccgccact tcgggccacg cggcgggatt gcgagtcaca
     2881 tcatgcggac caccggccag gccgctctgt tcaggatgac aggaactaca caagagggcg
     2941 ggggtgttgc gcaaaaactc ggtgaatttc tcagtgtgcg gatcgtggca ctcggcgcac
     3001 gcgacgtggc cgtggtttac gtcgctcaac actgttgcgt gtgcgacccg gccgggttga
     3061 tggcaggtta tgcacttgcc gccgccgggg tccagcgcgc taacctcggg tgcgcgggcg
     3121 tagcggtgga acaggtgaca ggagctgcat gggccgccgc tatgggccgt catgcccagg
     3181 cggttgcgct cctggggaaa gttctggcgc aagtcatgcg gcgagccgag caggacgcgc
     3241 ttgtcctcat gacagcgcag gcacatctgc ccctccgcca aatcgtaagc gagcaggaag
     3301 cgttgtcctt ccgcttcgtg cagccggtgg caggacaggc agatgagccg gccgtccggg
     3361 ccgagctgcg tgcccatctc gcgcacggcg gcggcctgct cggcgttgac gaccgggctc
     3421 agcgggtgct cagcgcggcc ttcgcgaaac atccccgggc gcatctgctc gtggcatgag
     3481 aggcaaagct ggtttgattc ggtgcccatg acgagcaatc gctcgtacgg ggcgccgtgc
     3541 ggagtgtggc agacctggca cgtcagttcg cgcgggtttg ggccgacccg ggctccggct
     3601 tgcagcagca cgtccggaac cggccatggc atgccgccgg tcgggtgcat gcccgcttcg
     3661 cggccgcgcg tcttctcaat gtgacaggca gcgcacagct cgctggcgag gttaggcacg
     3721 cgcaaaaaga ccgcggtcgc gatatcgccc gtgggctggc cggaaacgtg cgccgagtgg
     3781 cacgttcgac agacgatctt gccgtcggcc aacggcagga atgcgggcac gtccattccc
     3841 ggcggcggct cgacgtcggt gctatggcca tgttccagcc agacgcggcg gcgcgaatcg
     3901 accacggagc cgtcgtggca ggagaggcac atttcggcct gccccgcagg tggtatttcc
     3961 gtaaccggcg gcagcgacat caatacggtg ctttgcccgc ttgcgaacgg cgccagccat
     4021 tccaggtggc aacgagtgca ggcctgcggt tgcgcggcgc ggccggggcg ggcgggtctt
     4081 gccgccgggc gaacaggggc cttcgggttc gattcgattg tgaatttccg cacgcggttc
     4141 gcggtcagct cggcgacata caggttgtcg tcggcgtcga gcgcgatgcc gcatggcatt
     4201 tcgagcttga gcggctcgcc cgccgggtcg cgtaggacat cgatcagggt gccggtgatc
     4261 tcaaatatct gcaccacccc catgatgttg tcggccaccc acacgtttcc ggccgagtct
     4321 acggcgacgc cctttgggcg gtagagctgc ccaaggtcga cgccgaatgt gccgagcgtg
     4381 ccgaccgcct ggccttcggc gttgtggact tgtacacggc cggtgaccgg ctcggttacc
     4441 agcacgtctc cggtcgcgct cgtggccagc atgaagggat agaagtattg accgagcgat
     4501 tcgccggggc ggccgagcgg tcgcgatagt ccggtgcgca ggtccacgtc cagcaggtga
     4561 tggttgtggt tgtcggccaa ccaaaggcga cggccatccg ggctgagggc caggtcggtg
     4621 atgtcgggcg gcggcgcgcc gggcggttgg ctaatcagaa tttcgcgcgc cagggcgccg
     4681 ttcggggcac ggacgaggat gcggccgttg ccgctgtccg cgatccatag cgatccggcg
     4741 gcatccagct tgaggctaat cggccgagag agcgtctgtt gctcgatagt gtggatttct
     4801 tcctggatct cgcccgtagg ggcgaagacc aggatgcggt cattcacgcc gtcggcgacg
     4861 tacacgacgc catccggggc gacactgacc gcggtgggca ggcgcatcgg cgggtcggcc
     4921 gggtattcaa aggtcgggcc aggtgtggcg acgaatcgca gcgggcctgc cacagcgctt
     4981 gcagaaaacg ccaggctgat gaacacgcat gcgaggcggt tcaacgtcgt agcccggcta
     5041 gtgtggcgca tgctctttct cgatccggcg ctttctcgac ccggtgctct tgtacagccc
     5101 gggtcgctat tatatgagta atcgtgcggc cctcgcactg gccgcggcga gtctttgtga
     5161 tctccaagca ggagggagga gacggtgccc gacggccagg agcagcatgc gggaccggaa
     5221 ccgtcgtccg ggtcgaacca agcgggcgag aggccgccgg atgccggggc aaagcggccg
     5281 cgctggcggc ggcgctggcg caagctggcg atcgcggctg cggtcttcgt tttgctggcg
     5341 gcgctgggga tgggcggcgc cgaatactac acgtcgcggc cggatttctg cgcgtcgtgt
     5401 cacatcatgg gaccgtatta ccggtcctgg agccgggatg ttcacagcca gaaggtgagc
     5461 gtgtggtgcg ttgagtgcca ctacgcccca ggcgagcagc acaccatcca cgccaagttc
     5521 aaggggctgt cgcaactggc cagctacttc agcggccggt acggggccag ccggccgcgg
     5581 gcacacgtga acgacgcgag ctgtctgacg gcgagctgtc acggagaccg cgaatacctg
     5641 accacggcgt tgctgatcgg ggagccgcgg gtcgagaagc gcatcatcgg cggccaggag
     5701 agtgaggtgg tgcgcacgcc gaccgtgacg tttgtgcacg agaagcacct cgatgtgcag
     5761 agtaagctgg ctgagaatca ggcgcagttg gagcaggtgc gacagcgact gcggcgtgcg
     5821 gcgaagcccg aggagtatga agcgatcgtg accgtggccc ggcaggttgg tccggccccg
     5881 cagtgggaga aagacctgcg cgggaaattt gagcagggct tgccggaact tctggccgat
     5941 gctctggaat tggcccgttt ggagcaccgg cagacgcgtt tgcggcagtt ggccgggctt
     6001 aactgcgccg cttgtcacgg ctatgacggt tcgggcgagc atcacctctt gccggccgat
     6061 ctgcagactt gcttcatttg ccatttcaat aaccaggcgt ttaacaccga cacgggcgag
     6121 tgcctgaaat gccacgagcc gccgacgcgc aagattctcg tacacgacca ggcggtcacc
     6181 gcggcgtggc aacgggaatt cccggagacg acgcagccgg ccgggccggc cttgatggac
     6241 catcgcgaga ttgtggagcg cggcgtcaat tgcgccagtt gccacttgga cgtgatccag
     6301 gggcaggcca cggttacgga gcgcgattgc agtaactgcc acgaccaaga gcgttatctt
     6361 gcggagtttg cgacgcgcga tacgagcacc gtggaagaat atcatcgcgt gcacgtgaaa
     6421 gcgcagcgcg cccgctgcgt ggattgtcac cacgtcattc agcacgagtt gatcgaccca
     6481 acgcacgtcg ccacgagcgc aggctttttg cggccgatcc tggacgactg ccagcattgc
     6541 caccccaacc accaccacga gcaggtggag ctgctgatgg gcattggtgg ggtgggggta
     6601 gggcggccca tgccgaacgc gatgtttggc tcgcgcttga actgcaccgg ctgccatacg
     6661 cggccggcgc tggacatgaa aggtgccgag ttgattgcgg cgtccgagca gacctgcatc
     6721 gactgccacg ggtcggacta ccagcggctc ttcgagcagt ggcgtgccga agttgccttc
     6781 tcgttggggg aggcggagac ggctcttgag cgcgtcgaga agcgaattga cgagctacag
     6841 tcccgcgggc tggagattcc tgccgaggtg gccgacctgc ttgccccggc gcgccggaac
     6901 attctatttg tgaaggccgg caacggcatt cacaacaaga actacgcctt gcgactgctg
     6961 gatttgagca ttcgcgatct ggacgacgcg ctggggattc tgacgcgcga agggcgtcca
     7021 agattcggcc aacaatgatg ttgcctaatc ctggccgact gaacggcggg cgcgcccagg
     7081 cggcaagaca ccgcaacgct cactccgctc gtgcgagaat ccgtctcgca cgtcctccgg
     7141 cgggaatccc ttcccgcgcc cgccggcgcc ctcgccggcc cttcttcacc attccggcag
     7201 atcgacttcg atgcagcttg ttccctcgcc ggcatacgcc gctgctgagc tgaaccgcca
     7261 atgttccggg cgttggacca gccccttgcg gcacggattc gcgtgcaggt agtcgagctt
     7321 ctgctggaat acctcgcgtg accagatcgc ctgcgggcgg aagccgtctt gccagagccc
     7381 cagttcaccg cgcccgcggc gtgcctgttg ggttgcatac tgcagccaat gcagcagtgt
     7441 cagttatcac ttaaaccccg ccactgatta tcacttcaaa agcagccatt ttgaggaaca
     7501 cgcacct
//
LOCUS       DAOSYS010000181         7457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8419_length_7457_cov_5.349635, whole genome shotgun sequence.
ACCESSION   DAOSYS010000181 DAOSYS010000000
VERSION     DAOSYS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8419_length_7457_cov_5.349635"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLU99_11245"
     CDS             complement(<1..953)
                     /locus_tag="PLU99_11245"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOO17670.1"
                     /translation="MKIATAFTRAVNGDETAISLATRIRSCLGDLPVDLGFVFASAHY
                     EDELQRLALELHDLLAARTFVGVTAEGVIHADQEYESQPALALWAANLPDVTVRSFHL
                     AESDLERLQDPEPFREHVGVPADVDPNFILLGDPFTFSATPGLVTLLDQLNHVYPGRP
                     VLGGMASAADEPGQNLLVFEGQTLRHGMVGVALWGNIDLEMVVSQGCRPIGRHLVVTR
                     ADGNIIHELSGKPPLEVVKQLLVNAPARDRLLLQRRGLLVGYAINEYQKEFAQGDFLI
                     RLPIGFDPESGAMAINDLIRTGQTVQFQVRDESSATNDLDHA"
     gene            complement(1255..2268)
                     /locus_tag="PLU99_11250"
     CDS             complement(1255..2268)
                     /locus_tag="PLU99_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOO17671.1"
                     /translation="MFATFRRQPVQPDEKRMTLGEHLDELRGCLLRSLVALVLLSIPC
                     IFFARYLLEVLVRPLVLALRANNQPETLLATSPAETLLVYVKVVLISALILGGPYIIY
                     QLWTFVAAGLYPNERAWVHRLTLPSVLLFLLGVAFMYLFALLVSLNFLVGFGNWLPLP
                     RANPNALEQRLLSVSPAAPATTQPALENWPTVPVVAQDPAEPPVGAIWFNVNEHRFKV
                     RQPDRVYSYQFQPDQQRAMVSSHFKIGEYLSFVLLLTIAFGVAFQLPLVVVFLARTGL
                     VPVETMRRYRKVAILLIVFIACMLAPPDLLSHLLLSGPMLILFEIGVLIAARQTTPAA
                     PAD"
     gene            complement(2482..5265)
                     /locus_tag="PLU99_11255"
     CDS             complement(2482..5265)
                     /locus_tag="PLU99_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009091541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO17672.1"
                     /translation="MRWNYPLIVFSHLRWNWVYQRPQHLLSRLAARQRVLYVEEPVHD
                     SGAYRPFWHFHRPVRNVLVCQPHIPTPHAGFDTPELVTLLLELVRREDVPDHLAWMYT
                     PMALPLARRLKPQAVIYDCMDELSAFHNAPPELAQRENELFAWADVVFTGGPSLYEAK
                     RSRHPNVHCFPSSVDVKHFGQARGTMAEPPDQAKLPRPRLGFFGVIDERLDRDIVGAL
                     AAARPHWQIILLGPVTKIDPAALPQHPNLHYLGQRPYEELPGYLQGWDVCLLPFARNQ
                     ATRFISPTKTLEYMAAEKPIVSTPIRDVAEPYGELVYLGDTAEAFVAACEAALAAPPH
                     EREQRAAGMRQVLSRTSWDSTVSAMEELIHQAVLNRSSTLQTAPVTATVAPSATAAPH
                     TATPASRRAPERSHELVIIGAGPTGLSAAYHAGPGTVLLEKESTIGGWCRSFTAKGFT
                     FDYAGHIMFSNDPYVHELYEKLLGDNVHWQDREAWVYSKGVYTRYPFQGALYGLPPEV
                     IKECLVGAIQARFGPLKADTPAPPASAARAGGSARRQRLTSRPGPLGAKPQVSDCCAD
                     GVLESTAPLASPGGADGNGTKAGQPANFEEFIYKVWGEGIAKHFAIPYNRKLWAVPLN
                     EIETSWLGGRVPLPDLEEMIEGALHPTPKPMGPNARFGYPLRGGFQALLNGFLPYVRH
                     RLRLNSAVLRISPSQRSLTLATGEQMHYQQLISTMPLPILVRMMGDEVPPEVRRAAAA
                     LRHVSVRCVHLGVGREKLTEKHWIYYPEDTVFHRIFVQGNASPHCNPPGGFGLTCEIT
                     YSPAKPLPCEGDALVERCVADCRRVGLIGPHDPIVVSVQCDLPYAYVVYDHARAQNVQ
                     VIRQWLEDHDVHPAGRYGEWEYYNSDHAFLAGKKAIEAVRERQRTRPPMPVSASGTLL
                     PTRPLRGAGASPR"
     gene            complement(5472..7343)
                     /locus_tag="PLU99_11260"
     CDS             complement(5472..7343)
                     /locus_tag="PLU99_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOO17673.1"
                     /translation="MDGNTSSRKQIGYPRPQLRRANWTPLNGRWDFALDRDARWSEPR
                     QVRWDKTIVVPFAPETTASGIGEEGFFHACWYRRQFERPLLQPGERLILHFGAVDFAA
                     QVWVNGCMAVRHEGGYTPFSADITDLLDDGPAQTLVVRAEDIPNDLTQPRGKQDWQLN
                     PHSIWYPRTTGIWQTVWLEVVPETSIGFLRWTPSLQHWALGVEVRLAGVPRDDLRVRI
                     ELEADGKLLSRDTYAVISGEVHRGIVLSDPGIDDYRNELLWSPSHPTLIEARLELIDG
                     RGEIVDDVYSYTALRSIGVQGNRFVLNGRPLLLRMVLDQGYWPDTGLTPPDDAAVRRD
                     VELIKAMGFNGARCHQRVADPRYLYWADRLGLLVWGEMPSAYRFSKRSIMRVTREWTE
                     VVERDVSHPCIVAWVPLNESWGVPDLPDTPAQRHYVQALYHLTKTIDPTRPVIGNDGW
                     ESVATDIIGIHDYDSKPRRMVERYGGPDFSIARLAEQERPQGRILVLEPESHLKKPLM
                     LTEFGGIALSNSGDAKSWGYSEARDADELADRYAMLLAAVRSLHLIAGFCYTQFTDTY
                     QEANGLLYADRTPKFPIEEIALITAGEIPDATGGETKWRERLMQAQAKLSDEDQRIG"
BASE COUNT         1409 a         2237 c         2448 g         1363 t
ORIGIN      
        1 gcgtggtcca ggtcgttagt ggcggagctt tcatcgcgca cctggaactg gaccgtctgc
       61 ccagtacgaa tcaggtcgtt aatcgccatg gcaccggatt cggggtcgaa gccgatgggc
      121 aggcggatca ggaagtcgcc ctgcgcgaac tctttttgat actcgttgat ggcgtaaccg
      181 accaggagcc cgcggcgttg cagcaatagg cggtcgcggg ccggcgcgtt gaccaggagt
      241 tgcttgacga cttcgagcgg cggtttgccg ctcagctcgt ggatgatgtt tccatcggcg
      301 cgtgttacca caagatgccg gccaatgggg cggcaccctt gcgacacaac catttcgagg
      361 tcaatgttgc cccagagggc gacgcccacc attccgtgcc gcagcgtttg gccctcgaag
      421 accagcaggt tttgaccggg ttcatccgcg gccgaggcca tcccgcccag caccgggcgg
      481 cctgggtaaa cgtggtttag ttgatcgagc agcgtcacca gtcccggcgt ggcgctgaag
      541 gtgaaaggat cgccgagcag aatgaaattg ggatcgacat ccgcgggaac acccacgtgc
      601 tcgcgaaagg gttcgggatc ctgcaggcgc tccaggtctg actccgccag gtgaaatgat
      661 ctgactgtca cgtcaggcaa gttcgcagcc cacagcgcaa gcgccggctg gctctcgtac
      721 tcctggtcgg cgtgaatgac gccttccgcc gtgacaccta cgaacgtgcg cgccgccagg
      781 agatcgtgca actccagagc taggcgctgg agttcgtcct cgtaatgggc gctggcaaag
      841 acaaaaccaa gatcaaccgg gaggtcgccc aaacaagagc gaatgcgcgt ggcgagagat
      901 atcgccgtct cgtccccgtt aaccgctctg gtaaaagccg tggcgatttt caccgtcgag
      961 tctccggcac tattctatga acggcaaatc cgattcacca cgaacgcgga gaagcctacc
     1021 gggcaagcgg aaagcgccaa cgttaactcc ccataaacga tccggagcgg ccgcataaga
     1081 ccgctccggt cctcctctcc tctactctcc tgggcgcaaa aggccgcagt cggcgagtcg
     1141 ccacggcgac cgatacagtg ccgaacaaga agttcagttt accatcccgg cccatggaac
     1201 tcaataaatt tctgtagcgc tttcgcctta cagggcactg cttttaagcc gcggttagtc
     1261 ggccggggcc gccggggtag tctgccgagc ggcgatcagt actccgatct cgaacaggat
     1321 gagcatcggc ccggagagca ggaggtggct gaggaggtcc ggcggggcca gcatgcaggc
     1381 gataaagacg attaacaata tcgccacctt ccggtaacgg cgcatggttt caacgggtac
     1441 cagaccggtt cgggccaaaa agacaaccac taggggcaac tggaaggcca ccccaaatgc
     1501 gatggtcaga agcagaacaa agctcaggta ctcgccgatc ttgaagtggc tcgacaccat
     1561 ggcccgctgc tggtcgggct ggaactggta ggagtagact ctgtcaggct gacgaacctt
     1621 gaagcggtgc tcattcacgt tgaaccaaat cgctccgacc ggcggctcag cggggtcttg
     1681 ggcaacaacc ggcacggtcg gccagttttc gagtgccggc tgggtggtgg caggcgctgc
     1741 gggcgaaacg ctgagcagcc tttgctccag cgcgttgggg tttgcgcgcg gaagcggcag
     1801 ccagttgccg aaaccgacga ggaaattaag gctcaccagc aaggcgaaaa ggtacatgaa
     1861 agcaacgccg agcagaaaca ggagaacgct gggcaaggtc aggcggtgga cccaggcgcg
     1921 ttcattggga tacaggccgg ctgcgacaaa ggtccagagc tgatagatga tgtaggggcc
     1981 gccgagaatg agggcggaaa tcaggacgac tttgacgtag accaggaggg tctcggccgg
     2041 gctggtggcc agcaacgtct cgggttgatt gttggcccgc agagccagta caagcgggcg
     2101 taccaacact tcgaggaggt agcgggcgaa aaagatgcac gggatgctca ggagcactaa
     2161 tgccaccaag ctgcgaagca gacagccccg caactcgtcc aggtgctccc ccaacgtcat
     2221 acgcttttcg tccggttgga ccggctggcg ccgaaacgtg gcaaacatgg cggaatgata
     2281 ccgccaaaaa tcgaacgcgc cagccgctcc cgcccggcaa aatcagccgc cttcgatcac
     2341 gctcgatacg cccactacca cacgcgcgag gatacacccg agcgcctgga ctatgagggc
     2401 atggcgcgtg ttgtggcggg gctcgagcga atgctcatcg agctggcggg cggctcgcgc
     2461 cgctcagcca tagcccctct cctagcgcgg cgacgcgccc gcgcctcgca acgggcgcgt
     2521 gggcagtagc gtcccagacg cgctcaccgg catcggcggc cgcgttctct gtcgctcacg
     2581 cacggcctcg atcgcctttt tgcccgcgag aaaggcgtgg tcggagttgt agtactccca
     2641 ctcgccatag cggccggccg gatgcacgtc atggtcttcg agccactgac gaatgacctg
     2701 cacgttttga gcgcgggcgt gatcatagac gacgtacgcg tacggcaggt cgcactgcac
     2761 gctgaccacg atggggtcgt gcgggccgat cagtccgacg cggcggcagt cagcgacgca
     2821 gcgctcgacc agggcatcac cctcgcacgg caacggcttg gcgggtgagt acgtaatttc
     2881 gcaggtaagg ccgaagccgc ccggcggatt gcaatgcgga ctggcgttgc cctgcacgaa
     2941 gatgcgatga aagaccgtat cctccggata gtaaatccag tgtttctcgg tcagtttctc
     3001 gcggccgacc cccaggtgaa cgcagcgcac cgaaacgtgt ctcagggctg cggccgcgcg
     3061 gcgcacttcg ggcgggacct cgtcgcccat catgcgcacc agtatcggca ggggcatggt
     3121 gctgatgagt tgctggtagt gcatttgctc gccggtggcc agagtcaggc tgcgctggct
     3181 gggcgaaatg cggagcaccg cgctgttcag gcgcaagcgg tgccgcacat agggcaagaa
     3241 gccgttgagc aatgcctgga acccgccacg gagcggatat ccaaagcggg catttgggcc
     3301 catgggcttg ggagtggggt gcagggcgcc ttcgatcatt tcctcaagat cgggcagcgg
     3361 cacgcgtccg cccagccagg aagtctcgat ctcgttgagg ggcactgccc agagtttgcg
     3421 gttatatgga atggcgaaat gttttgcgat gccctcgccc catactttgt agatgaactc
     3481 ttcgaagttc gccggttggc ccgctttggt tccgttgccg tcagcgccgc cgggggaggc
     3541 tagcggcgcc gtgctttcca gcacgccatc ggcgcagcag tcactgactt gcggcttggc
     3601 gccgagcggc ccggggcgcg aggtaagccg ctgccggcgt gcgctcccgc cggcacgcgc
     3661 tgcgcttgcg ggcggggccg gcgtgtcggc tttaagcggg ccgaagcggg cctggatggc
     3721 gccgaccagg cattccttga tcacctccgg cggcaacccg tacagcgcgc cctggaaagg
     3781 atagcgtgtg tagacgccct tgctgtagac ccaggcctcg cgatcctgcc aatggacgtt
     3841 gtcgcccagc agtttctcgt acagctcgtg cacgtagggg tcgttcgaga acataatgtg
     3901 gccggcgtag tcgaacgtga accctttggc ggtgaacgag cggcaccagc cgccgatggt
     3961 gctttctttc tccagcagta cggttcccgg gccggcatga taggcggcgc ttaggccggt
     4021 cggtccggcg ccgatgatga ccaattcgtg gctgcgctcc ggggcgcgcc tggaagccgg
     4081 cgtcgccgtg tgcggcgctg cagtcgcgct cggtgcgaca gtagcggtaa ccggcgcagt
     4141 ttgcagtgtg cttgacctat tcagcacggc ttgatgaatc agctcttcca tcgcagagac
     4201 tgtactgtcc caggaggtgc gcgaaagcac ctgccgcata ccggccgcac gctgctcgcg
     4261 ttcgtggggc ggcgcagcga gggccgcttc gcaggcggcc acaaaggcct cggcggtgtc
     4321 gcccaggtac accaactcgc cgtagggctc ggccacgtcg cgtatcggcg tgctgacgat
     4381 gggcttttcg gcggccatgt attcaagggt tttggtgggg ctgatgaagc gcgtggcctg
     4441 gttgcgtgcg aagggcaaca gacacacatc ccagccctgg agatatcccg gcagctcttc
     4501 gtatggacgt tgtccgaggt agtgtaggtt cggatgctgc ggcagcgcgg ctggatcgat
     4561 cttagtgact ggaccaagca gaatgatctg ccaatgcgga cgggccgcgg ccaacgcccc
     4621 gacgatgtcg cgatccaacc gttcatcaat aacgccgaag aaccccagcc ggggtcgcgg
     4681 tagcttggcc tgatcgggcg gttcagccat ggttccgcgc gcctggccga agtgtttcac
     4741 atccacactg cttgggaaac agtgcacatt tggatggcgg ctgcgcttgg cttcatagag
     4801 gctggggccg ccggtaaaga ccacgtcggc ccaggcgaac agttcgtttt cgcgctgcgc
     4861 cagctccggc ggggcattgt ggaaagcgga taattcgtcc atgcagtcat agataacggc
     4921 ttgcggcttc aagcgccgcg ccaacggcaa agccatcggc gtatacatcc aggcaaggtg
     4981 gtcgggtacg tcctcgcggc ggacaagctc gagcaagagt gtcacgagtt ccggcgtatc
     5041 gaatccagcg tgcggcgtcg ggatatgcgg ctgacaaact aagacattcc tgaccggccg
     5101 gtgaaaatgc cagaatggtc gatacgcgcc ggaatcatgc actggttctt cgacgtagag
     5161 tacccgctgt cgcgcggcca gccgcgacag cagatgttgc ggccgctggt agacccagtt
     5221 ccagcgcaag tgagagaaga cgatgagcgg gtaattccat cgcatgaagt tctcctgcgc
     5281 gcgcattgcg agcagcgcgc ggcatatgct caagttttgg cccagcaagg tcggattgca
     5341 ggcgatccgc gcggactcgc tttaactata tttccgggtg actcgaaaac aaatgaaatc
     5401 gacgtaaacg cagcattgag accgtacatg ctacgtttac gccggtgtgc tctggtgtca
     5461 gagtttgcag cttagccgat tcgctggtct tcgtcgctga gcttggcctg cgcttgcatc
     5521 aagcgttcgc gccatttggt ctcgccgccg gtcgcgtccg ggatttcgcc ggccgtgatc
     5581 aaggcgattt cctcgatagg aaacttcggg gtgcggtcgg cgtacagcag gccattggcc
     5641 tcctgataag tgtcggtgaa ttgggtgtag cagaacccgg cgatcaggtg cagagagcgg
     5701 acggccgcca gcagcatggc gtagcgatcg gcgagttcgt cggcgtcgcg cgcctcggaa
     5761 taaccccagc tttttgcatc gccactgttg gaaagcgcga tgccgccgaa ctcggtcagc
     5821 atcagcggct ttttcaaatg cgattcgggt tcgagcacca ggatgcggcc ctgcgggcgc
     5881 tcctgctccg ccaagcgtgc gatcgagaaa tcggggccgc cgtagcgctc gaccatgcgg
     5941 cgcggtttgc tgtcgtagtc gtggattcca atgatgtcgg tcgccacgct ttcccagccg
     6001 tcattgccga tgacgggccg agtggggtcg atggtttttg taaggtgata cagcgcctgc
     6061 acgtaatgcc gctgggcggg cgtgtcgggc aggtcgggta cgccccagga ttcattcagc
     6121 ggcacccagg ccacgatgca cggatgactg acgtcgcgtt cgacgacttc ggtccattcg
     6181 cgggtgacgc gcatgatcga gcgcttgctg aaacgatagg cgctgggcat ctcgccccac
     6241 accaataacc ccagccggtc ggcccaatac aggtagcgcg gatcggctac gcgctggtgg
     6301 caccgcgcgc cgttgaagcc catcgccttg atgagctcga catcgcgccg cacggcggca
     6361 tcgtcgggtg gcgtcagacc cgtatccggc cagtagccct gatccagcac catgcgcagg
     6421 agcagcggcc ggccattcaa cacgaagcgg ttaccttgca cgccgatcga gcgcaaggcg
     6481 gtatagctgt aaacgtcatc aacgatttcg ccgcgcccgt cgatcagctc gaggcgcgcc
     6541 tcaatcagcg tgggatggga cgggctccac agcagttcgt tgcggtagtc gtcaatgccc
     6601 gggtcagaca gcacgattcc gcggtgcact tcaccggaga tgacggcgta cgtgtcgcgg
     6661 ctgagcagct tgccatcggc ctctaactcg atgcgtacgc gcaagtcgtc gcgcggtacg
     6721 cccgccaggc gaacttcgac gccgagggcc cagtgctgga gtgagggcgt ccagcgcaga
     6781 aagccgatgg acgtttcggg gacgacttcc agccagacgg tctgccagat tccggtggtg
     6841 cgcgggtacc agatggagtg cgggttcagt tgccagtcct gctttccgcg cggctgagtg
     6901 agatcgttgg ggatgtcctc cgcgcgcacc acaagcgtct gcgccggccc gtcgtccagc
     6961 aagtcggtaa tgtcggccga gaacggcgtg tagccgccct cgtgccgtac ggccatacag
     7021 ccgttgaccc acacctgggc ggcgaagtcg accgctccga agtggaggat cagccgctcg
     7081 cccggttgca ggagtgggcg ctcgaattgg cggcgatacc agcaggcgtg gaagaagcct
     7141 tcttcgccga taccgctggc ggtagtttcc ggggcgaagg gcacgacaat tgtcttatcc
     7201 cagcgtactt gccgcggttc gctccagcgc gcgtcgcggt cgagcgcaaa atcccagcgg
     7261 ccgttgagcg gggtccaatt ggctcgtcta agctgcgggc gcggatagcc gatctgcttt
     7321 cgcgacgagg tgttgccgtc catacgagct ccgagaatcg ccggctgcct gggtattatt
     7381 cgtcgcccat gccggcgggt cctgcttgcg taagggacct gaatcgacga cgcgctgctc
     7441 gcccccgcga tcgtcta
//
LOCUS       DAOSYS010000182         7367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8568_length_7367_cov_4.479759, whole genome shotgun sequence.
ACCESSION   DAOSYS010000182 DAOSYS010000000
VERSION     DAOSYS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8568_length_7367_cov_4.479759"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLU99_11265"
     CDS             complement(<1..508)
                     /locus_tag="PLU99_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17674.1"
                     /translation="MMSFIKQKTSLLLVLAMAGTGIAVGQTCPGGLVADQTGQLGPSA
                     PGGRFGIAVAVDGGTAVIGDRNHNAEAGAVYVFDFDGANWTEQGRLTASDGLTGDHFG
                     MALALEGDTLVVGASGDDEVASASGSVYVFVRSGNEWVEQARLNAADPGLGDYFGLSV
                     AISGDTIAV"
     gene            1408..1866
                     /locus_tag="PLU99_11270"
     CDS             1408..1866
                     /locus_tag="PLU99_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOO17675.1"
                     /translation="MTNIEASGQKQERHLCERCAIDEGLMQPAKPAVDINDLLESFVA
                     AGKSASGPNVGNLVCENCGISYIEFRNQGLLGCASDYDAFAEPLARLLERAHEGGTHH
                     VGKAPKSVGVRRTTLQDVRRLKRALSEAVAAEDYERAARLRDQIRKMEES"
     gene            1863..2921
                     /locus_tag="PLU99_11275"
     CDS             1863..2921
                     /locus_tag="PLU99_11275"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOO17676.1"
                     /translation="MSPLDAVARQAGEWLRGVGPQHEIVISTRIRLARNIKDFVFLSR
                     ADADTRAEIADTIQSAIRRGNGLKDLVHVDVEKLDEIDRALLVERHLISRQHAEGTGA
                     RRVAFDTSEVSSIMINEEDHLRLQVMRSGFQLNEAWDQINSIDDALEQTLDYAFHPKY
                     GYLTACPTNVGTGIRVSVMLHLPALRLTHELDKVAQAAKDLRLAIRGLHGEGTEALGD
                     FFQISNQITLGKSEAEIIDEFRDIIIPKIVEYESAARQSLLRNRLHVLDDKIWRAMGA
                     LQNARLISSNEAMQYLSHVRMGLHVGRLKNVELQTLNELFLQMQPAHLQKLQGERLNG
                     EQRSVARAALIRARLSSN"
     gene            3098..3889
                     /locus_tag="PLU99_11280"
     CDS             3098..3889
                     /locus_tag="PLU99_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17677.1"
                     /translation="MSKPKVACVGGGVVGSAIRRLCGPETVVYDIRQSPGCTQDKGQV
                     NACDVCFISVPTPMAADLSCDTSIVEECVAWIECPLIIIRSTVAPGTTDRLSVEYKKN
                     IVFQPEYLGETPAHIFGNMAEREFVVLGGKPELTSRAADFYKHYYNAYVRFYFCDAIT
                     AEVCKYMENAFYAVKVTFVNEFYDVAKAHGVDFNVLREVWLADTRISRDHTFVYPEAR
                     GFSGKCLPKDCNAIVQSCLQRGYTPEFMQACLRINDKFQAQNKPG"
     gene            4101..5495
                     /locus_tag="PLU99_11285"
     CDS             4101..5495
                     /locus_tag="PLU99_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOO17678.1"
                     /translation="MKHSSNPSMAAPAEVYRQRRARLATQIRRPLVIFSGHAPARNYA
                     TNPHPFRAGSTYLYFGGPPLEHAALVIEPESNGDEGCRLVRPVLGPEDKVWFGPVPTD
                     DELSAACGIDRSRFMTASDLEWLLAGYEAAAIVPPAPQSIARAAELGLPSANEQEIRT
                     IVDMRLIKDEHELDAMRRSAEIAIEAHRAALAACVPGGREADVAAAFTAVLVANQSAH
                     SFNPIITIHGEVLHSEGHPHDLRDGALLLIDAGAEEPTGYASDITRTIPVNGQWTPMQ
                     RQLYEIALKAMESAIAASVPGKRFREVHDIAARVICEGLVEADLLRGDPAELAARTAH
                     ALFFPHGIGHLIGLDVHDMEDFGDLAGYPPGRTRPTEFGSKFLRMDRDLAPGMTVTIE
                     PGIYLVPAIWATDDLVRPFADAVNRAKVEALLETEFGGIRVEQTICIRESGQPEVLTE
                     GLPSAPDEIVQLLG"
     gene            complement(5644..6165)
                     /locus_tag="PLU99_11290"
     CDS             complement(5644..6165)
                     /locus_tag="PLU99_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HOO17679.1"
                     /translation="MFRHWHHALGVAVLLTVTGLALADEDDVREMQRATAAAKVSLVQ
                     AIEIAEREVTGGKAVEVELDWKRGGPRIEVELVVGDNWKEVAIDAVSGRVLKVLDERA
                     DDPDDREELKRDKENLQAATVGFADAIARTEKEAGGKAVDVELTSSQGKPAYKVKLLV
                     DNKLATVRVPAND"
     gene            6673..>7367
                     /locus_tag="PLU99_11295"
     CDS             6673..>7367
                     /locus_tag="PLU99_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17680.1"
                     /translation="MRRSRTWSIPGSARGPADGGLTARGPLGGLSARGTALNGPLGRW
                     RLIAAAAILALALTGVFTIYFHWRRASLHWAITRGLELLEPANTPLRAAAVLDEWENE
                     TAGRWHNRRDELARRLFSDYPLTDERVRRLLTRVTGADNGQRREDWKEWLDTQRRLQA
                     GKPPKDRLRFDKLWEAPAGLTAWFSTILPLDGQIYVASLGTTFGSEHDPADGVVRVDG
                     RTGAAALIFSPPDG"
BASE COUNT         1361 a         2582 c         2167 g         1257 t
ORIGIN      
        1 cgacggcgat cgtgtcgcca ctgatggcca cactcaggcc aaagtagtcg ccaagtcccg
       61 ggtcggcggc attcaagcgc gcctgctcaa cccattcatt gcctgaacgg acgaaaacgt
      121 agactgagcc actggcggag gcgacttcgt catcaccact ggcacccacc acgagagtgt
      181 cgccctccaa cgccagcgcc atgccgaagt ggtcgccggt caatccgtca gatgcggtca
      241 gccggccctg ttctgtccag tttgccccat caaagtcgaa cacatacacc gcaccggctt
      301 ccgcgttgtg gttgcggtcc ccgatcactg ccgtcccgcc gtcaactgcg acggcgatgc
      361 cgaaccggcc gcccggcgcc gacggcccca attgccccgt ctggtccgcc accaggccgc
      421 cagggcaagt ctggccgacg gcaatcccgg tcccggccat tgccaggacc aacaatagac
      481 tggtcttctg cttgatgaaa ctcatcattc cgcctctcac atggtgctaa aacgtgctgg
      541 aacgccggtc tgcctgcgct cgcagaactg acccaaccgg cgcactctgc tgctggtatg
      601 agctcctctc cgcgattccg gaacgtgcgc ctactaccgg ccgcagccga tttcacacgt
      661 caccgggcca cccgcaatcc attgccgcga attatgagcg catccaatct ggttccactc
      721 aacgcgctca gagagccgca gacccgcggc cgcaaaacgt ctctcgaccc aagccgtttc
      781 tccccgcgcg gaacgatccg ccccgtggga taatcagatc gagacctcaa aacgccactt
      841 ctatcacttc tgtacacccc gagtcaagac ccaatggccg gcccaggacg tagtgcgtag
      901 cagagccggc tcgtgtcgag ccggaatgtg accagaggcg ccaagcgccc aggtcgcacg
      961 cccgacacga taggagtgct catgctggcg ctgcgggctc aaatcttggg agttcggacc
     1021 gggcgcggtc ggaccggcag tgcggctcgt tcaacggact cccggcgact gccggcggcg
     1081 ccggcatcgc aagccgtccg gcgaactccg tgacggacaa cgcgtaattg gcgcgccctc
     1141 gaacaccggg ccaccgccag cgccgccctg actggccgcc gccagacgta tcaccgccac
     1201 ggccgccggc tctcgccaaa agccacttcc gccgataaca tccagatatc agggtaagcc
     1261 cgccgaccgt gcccccgcct gccccgcctc ggaaacttga cgtggctgtg cgctaattct
     1321 acgatcgttt cggctggcta acccgcggtc aggagcgcgg cgatgggact ttgcgaacgc
     1381 tgcaggaagg cacaggcgac gttccacatg acgaatatcg aggcctccgg tcagaaacag
     1441 gagcgccatt tgtgcgaacg ttgcgcgatc gatgagggcc tgatgcaacc cgccaagccc
     1501 gcggtcgaca taaacgacct gctggagagc ttcgtcgccg ccggcaagag cgcctccggg
     1561 ccgaacgtgg gcaacctggt ctgcgagaat tgcggtatca gctacatcga gtttcgcaat
     1621 cagggcctgc tgggctgcgc tagcgattat gacgcctttg ccgagcccct cgctcgcctg
     1681 ctggagcgtg cccacgaggg cggtacgcac cacgttggta aagctccaaa gtcggtcggc
     1741 gtccgccgca cgacccttca ggacgtgcgc cgcctcaaac gcgccttgtc cgaggcggtc
     1801 gcggccgaag attacgagcg ggcggctcgc ttgcgcgacc aaattcgcaa aatggaggaa
     1861 tcatgagccc ccttgacgcc gtggcacgcc aggccggtga gtggttgcgc ggcgtgggtc
     1921 cgcagcacga aatcgtgatt tcgacccgta tccgcctggc acgaaacatc aaggatttcg
     1981 ttttcctgtc gcgggccgat gccgatacac gcgcggaaat cgccgacacc attcagtccg
     2041 ccatccgccg cggcaacggg ctcaaggatt tggtccacgt cgacgtcgag aagctggatg
     2101 agatcgatcg cgctctgctg gtcgagcgac acctgatcag ccgccagcac gccgagggca
     2161 ccggcgcccg ccgcgtcgcc ttcgacacca gcgaagtctc ctcaatcatg atcaacgagg
     2221 aggaccacct gcgcctccag gtcatgcgca gcggcttcca actgaacgag gcctgggacc
     2281 agatcaacag tattgacgac gcccttgaac agactctgga ttacgcgttt caccccaagt
     2341 acggctactt gaccgcctgc cccacgaatg tgggcaccgg catacgcgtt tcggtcatgc
     2401 tgcacctgcc ggccctgcgc ctcacgcacg agctggacaa ggtcgcccaa gccgccaagg
     2461 acctgcggct ggcgatccgc ggcctgcacg gcgaaggcac cgaagccctc ggtgatttct
     2521 tccaaatttc caatcagatc acgctgggga agtccgaggc cgaaattatc gacgagttcc
     2581 gcgacatcat catcccgaaa atagtcgaat acgagtccgc cgcccggcag agcctgctgc
     2641 gtaaccggtt gcacgtactc gacgacaaaa tctggcgcgc catgggggcc ttgcagaacg
     2701 cccggctgat cagctcgaat gaggccatgc aatatctgtc acacgtgcgc atgggcctgc
     2761 acgtggggcg cttgaagaac gtggagctgc aaacgctcaa cgagctattc ctgcaaatgc
     2821 agcccgcaca cctgcaaaag ctccaaggcg agcggctgaa tggcgagcag cgcagcgtag
     2881 cccgcgccgc cctgatccgc gcccgactta gcagtaacta gcgaagtaaa aagagccccg
     2941 gggaattcgg ctccaatctg gacgcgctgc gccttccgca tcttgcgttc ccacttgccg
     3001 tcaggcttga cctcccgccg cgcagccgct attccagtct ctctggttgc ccggtcggcg
     3061 cggccgggcc ggcgtatccg gagatagaga ggcgagcgtg tccaagccca aagtcgcgtg
     3121 tgtcggtggt ggcgtcgtag ggtccgcgat ccgcagactc tgcggccccg agacggtcgt
     3181 ttacgacatc cgccagagcc ccggatgcac gcaggataag ggccaggtca acgcctgtga
     3241 cgtgtgcttc atcagcgtgc ctacgccgat ggctgccgac cttagctgcg acacctccat
     3301 cgtcgaagag tgcgtcgcct ggatcgagtg cccgctgatc atcatccgct cgaccgtggc
     3361 ccccggcacg accgaccgcc tgagcgtcga atacaagaaa aacatcgtct tccaaccgga
     3421 atacctgggc gaaacccccg cccacatctt cggcaacatg gccgaacgcg agttcgtcgt
     3481 cctcggcggc aagcccgagc tgaccagccg cgcggccgat ttttacaagc attattacaa
     3541 cgcctacgtg cggttctatt tctgcgatgc catcaccgcc gaggtgtgca aatacatgga
     3601 gaacgcgttc tacgccgtca aagtgacgtt cgtgaacgag ttttacgatg tagcgaaggc
     3661 gcacggcgtg gatttcaacg tgctgcgcga ggtctggctg gcggacaccc gcatctcgcg
     3721 cgaccacacg ttcgtctacc ccgaggcccg cggcttctcc ggcaaatgcc tgcccaagga
     3781 ctgcaacgcc atcgtgcagt cctgcttgca acgcggctac accccggagt tcatgcaggc
     3841 ctgcctgcga ataaacgaca agttccaggc ccagaataag cccgggtagg gaaaggcgac
     3901 gctccggccg tgccggtcgc gggcggggcc acgctgggca gcgctggcga ataagatcat
     3961 cccggccggg tgcgcagccg ggtgccatgc ccaagctgta agcgcgggca tgcccttgtt
     4021 aacggcccaa cgtgccgggt ccggaactat tcgcgacggc atgcccgtaa cagccgaccc
     4081 acataattgc gagtccaaac atgaaacact catctaaccc cagcatggcc gccccagccg
     4141 aggtttatcg acagcgccgc gctcgcctgg ccacccagat tcgccgaccc ctggtcattt
     4201 tcagcggcca cgctccggca cggaactacg ccaccaaccc gcacccgttc cgcgccggca
     4261 gcacgtacct gtacttcggt ggcccaccgc tcgagcacgc ggccttggtc atcgagccgg
     4321 aaagcaacgg cgacgagggt tgccgcctgg ttcgcccggt gctcggcccc gaagacaagg
     4381 tttggttcgg ccccgtcccg actgacgacg agctgtcggc cgcctgcgga atcgaccgct
     4441 cgcgcttcat gaccgccagc gatcttgaat ggctgctggc cggctatgag gcggccgcaa
     4501 ttgtcccgcc cgcgccgcag agcatcgccc gcgcggccga actgggcctg ccttcggcca
     4561 atgaacagga aatccggacg atcgtcgaca tgcggctgat caaggacgaa cacgaactgg
     4621 acgccatgcg ccgctcggcc gagatcgcga ttgaggctca tcgcgccgcg ctggccgcct
     4681 gcgtccccgg ggggcgcgaa gccgacgtgg ccgcggcgtt taccgcggtg ctcgtggcga
     4741 accagtccgc gcactcattc aacccgatca tcactatcca cggcgaagtg ctgcacagcg
     4801 agggccaccc gcacgatttg cgcgacggcg cgctgctgct catcgacgcc ggcgccgaag
     4861 agcccaccgg ctacgccagc gacatcacgc gcactatccc ggtcaacggc cagtggacgc
     4921 cgatgcagcg ccaactgtac gagatcgctc ttaaggccat ggaaagcgcg atcgcagcca
     4981 gtgttcccgg aaaacgcttc cgcgaggtgc acgacatcgc ggcgcgcgtc atctgcgaag
     5041 gcttggttga agccgacctc ctccgtggcg acccggccga actggccgcc cgcacggccc
     5101 atgccctgtt tttcccgcac ggcatcggcc acctgatcgg cctggacgtg cacgacatgg
     5161 aggacttcgg cgacctagcc ggctacccac ccggccgcac gcgccccacc gaattcggca
     5221 gcaagttcct gcgcatggac cgcgacctgg cgccgggaat gacggtcacc atcgagccgg
     5281 gcatctacct ggttccggcc atctgggcga cggacgactt ggtccgcccc ttcgccgatg
     5341 cagtgaaccg cgccaaggtc gaagcgctgt tggaaaccga gttcggcggc atccgcgtag
     5401 agcaaaccat ctgcatccgc gaatccggcc agccggaagt gctcaccgaa ggtctcccca
     5461 gcgccccgga cgaaatcgtc caactgctcg gctagcacgg ccgggatggg tgatagggtg
     5521 ataaagtgat agagtgataa aggacgagct gccgaatcac tttatcactc tatcacctta
     5581 tcactttatc acgatcgtcg catcaagctt ctccccatcc gtcctgtcaa ccgagcactg
     5641 ccgttaatcg ttggccggaa cgcgcactgt ggccaactta ttgtcgacca gcagcttgac
     5701 tttgtatgcc ggcttaccct gtgaggaggt caactccaca tctaccgcct tgccgccggc
     5761 ctccttctcg gtccgcgcga tcgcatccgc gaatccgacc gtggccgctt gcaggttctc
     5821 tttgtcgcgc ttcagctctt cgcggtcgtc ggggtcgtcg gcccgctcat ccagcacttt
     5881 gagcactcgg ccggaaacgg cgtctatcgc cacttctttc caattgtccc cgaccaccag
     5941 ctccacctcg atgcgtggac cgccgcgctt ccagtcgagt tcaacctcga ccgccttgcc
     6001 gccggtgacc tcgcgctccg cgatctcgat ggcctgaacc agtgacacct tggcggcggc
     6061 ggtggcgcgc tgcatctcgc gcacgtcatc ctcgtccgcc aaagccagtc ctgtcacagt
     6121 gagcaacacg gcaacgccca gagcatgatg ccaatgacga aacatggtgg atttctcctg
     6181 tatattgcct acggtccaaa agcccggctg actgcccgga ttgtaggcgc aggcgattcc
     6241 cggggcgatg gcccgccgac ttgagcgact ccaacctgcc ccccctgcga acaccggccg
     6301 cttcagactg acttgcggtg aaggcgcctg ggacgcgcca cgcgggcaac tcagaccgtt
     6361 tcgttcacag tgtgaactgc cggcgaggat ggggtcaacg gcacttggtt ccgccccgtt
     6421 tcaaggctaa ccgtgatgcc gcctttactg acaagaaagt gctttcagct gcattacccc
     6481 tgtcggcttg ccattaggaa aactacctcc gccttggtcg ggccctccgc ctgcctgccg
     6541 cgccattcgc gttgccgccc tggttgcctt tcttcccttg cctcgtcggc cggttgcctt
     6601 tcgtacctaa ttacctacca tgcactcccc ggcccctgcc gcggcgggcg cgggccggtg
     6661 gggctgcttg acgtgaggcg ttcgaggact tggagcattc ccgggagcgc tcgtggaccg
     6721 gccgacggcg ggctgaccgc tcgtggaccc ttgggcgggc tgagcgctcg cggaacggcc
     6781 ttaaacgggc cgctcggccg ctggcgcctg atcgctgccg cagccattct ggcgctcgcg
     6841 ctgaccggcg tcttcaccat ctacttccac tggcgacgcg cctcgctgca ctgggccatc
     6901 acgcgcggct tggaattact cgaaccggcc aacacgccgc tgcgcgccgc cgccgtcctc
     6961 gacgagtggg agaacgaaac cgccggccgc tggcacaacc gccgtgacga actcgcgcgg
     7021 cgcctgttct ccgactaccc gctcaccgac gagcgcgtgc gtcgcctgct gacgcgcgtc
     7081 accggcgccg ataacggcca gcgccgcgaa gactggaagg aatggctcga cacgcagcgg
     7141 cgcctgcagg ccggcaagcc gcctaaagac cgcctgcgct tcgacaagct ctgggaagcg
     7201 ccggccggcc tgaccgcctg gttttcaacc atcctgcccc tggacggcca gatttacgtt
     7261 gcctcgctcg gcacgacctt cggctccgag cacgacccgg ccgacggcgt cgtccgcgtc
     7321 gacggccgaa ccggcgcggc cgcactcatc ttctcgccgc cggacgg
//
LOCUS       DAOSYS010000183         7193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8821_length_7193_cov_4.627487, whole genome shotgun sequence.
ACCESSION   DAOSYS010000183 DAOSYS010000000
VERSION     DAOSYS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8821_length_7193_cov_4.627487"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1152)
                     /locus_tag="PLU99_11300"
     CDS             complement(196..1152)
                     /locus_tag="PLU99_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17681.1"
                     /translation="MLAGVVLVFASPAWAGDSAELIKQGNEHYAAGRFAEALQAYEQA
                     NEEADGGNAAELLHNRAAAHFKLGNLAEARELWTRAIPLGDAKFEARTRYNLGNCDLA
                     DLVQQEGAGGTGMQELLERVISQYRDAIRLDPGLADARANLELVYRLKKQLEEQQPQS
                     QPSSQPSSQPSSQPSSQPDSQPSSQPSEQQEGQDQQEGQEQQQQGSEQDSQANENEDQ
                     SPSSQPTSQPSEEGEQEPQPESQPASQPATQPEEPQSEDLQEQQAEQQEEPAADQPPP
                     GVKMSRQEAERLLQKIRDAEKARRELLRQRERARHKPVDRDW"
     gene            complement(1197..2258)
                     /locus_tag="PLU99_11305"
     CDS             complement(1197..2258)
                     /locus_tag="PLU99_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO17682.1"
                     /translation="MSNLEFDQRWLWLLWVVLGTALIGVYGIWQRQRALRRFAAPGLL
                     PRLAPPLGWGRRLLRLGLIVACLVALVAAIINPRWGEQEQQVVRRNVDVLVLLDVSRS
                     MLARDIAPNRLERALLSIRDDLLPALGGDRIGLITFAGVPTLACPLTNDYGFFRLALS
                     DVSTESSPRGGTLIGDAIRKAGECFPGQLSTNKVVFLITDGEDHESYPVGAAAALWED
                     HRIPIVAVALGDEREGARIPVAKAGAGETYLEYDGQTVWSRANFDDLRKIAAVSDLNA
                     FVPVGTRNFDLGDIYRRVVVPKIEAQQKLESERVPRPSRYHPFAVAALLLLVIESLVR
                     EAPPAAMRAIALGQKRRAG"
     gene            complement(2255..3310)
                     /locus_tag="PLU99_11310"
     CDS             complement(2255..3310)
                     /locus_tag="PLU99_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO17683.1"
                     /translation="MTHHHLPLTPQWVQWPVPWVWALLGLALLPLLWWLWQQPSWRAA
                     LRFSDLTPLRAAGARWSAGLRRILPALRIAALACLIIAVARPQRPDESNQIFAEGIAI
                     QMVVDTSSSMRDTDLSPRGQPMTRLDVVKDVFRRFVLGDGDALPGRPNDLIGMIRFAK
                     YPDSVCPLTLDHSSLVEVLDKTQIVTRREEDMTAIGDALALGVSRLKDLKRTSGSGQQ
                     YTITSRVIVLLTDGEQNAGDLSPEQAGELAATYGIKVYTILAGTGAVRGFSRMPVDDE
                     PLKRVAELTGGRAYHARDRGSLLKIYEEIDRLERTKTEERRFVHWGELSRPWLVAAFA
                     CICLQVLLDSTVLRKIP"
     gene            complement(3307..4317)
                     /locus_tag="PLU99_11315"
     CDS             complement(3307..4317)
                     /locus_tag="PLU99_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17684.1"
                     /translation="MFLAAVLGGCDRGPAPESDEVRESKERGPVKFEVTVRPKAVWLG
                     DPFEIVLEVRAPEDYVVEFPAAQALPDLQIRGEETPDPRPAPEGGLEWRRVFRVESLA
                     SGTLEIPELIAKYGRKPEQAGEPVRFQNELQTDPLKVEVRSALTTQDSLMNPRDITGT
                     LSPKPSLKRLIAKGLLLAASIAALATAAVLAWIWFRRRQRNVPPVAPELVALQALEQL
                     GRRDWIEIGRARDYYYELTEIVRAYIERKFSLAAPEMTTEEFLNALAHKPSALPYDAY
                     RLRTFLEACDLVKYAAFRPNRTDADQALMTARAFVHETAAAAQRAQLARMQQQSGEGQ
                     AA"
     gene            complement(4410..5285)
                     /locus_tag="PLU99_11320"
     CDS             complement(4410..5285)
                     /locus_tag="PLU99_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007340032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOO17685.1"
                     /translation="MIPTEILKKVRRIQIVTSAMVNDVFAGQYHSAFKGRGMEFEEVR
                     EYQPGDDVRTIDWNVTARSGRPFVKNYREERELTVLLAVDVSASQQFGTREQLKSELV
                     AELGATLAFSAIQNNDKVGLILFTDRIERFVAPRKGMRHVLRVVRELLYHEPAGRKTD
                     VGVALESISRVLARRAVVFLISDFQTPSFTGPLQIVRHRHDVIPVIVRDERERRLPPV
                     RYVEVLDPETGAQMLVDTSSRAFRERFARLADERHAALITELRRMRLDAIEVATGESF
                     VEPLRRFFHRRGRRR"
     gene            complement(5452..6564)
                     /locus_tag="PLU99_11325"
     CDS             complement(5452..6564)
                     /locus_tag="PLU99_11325"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17686.1"
                     /translation="MKLTAAFVLVCAVGVAIVAVRVVRVWWQGQAAPVAVHAEVGHVT
                     EASTNVSAAEEDGTPGAIAEPDETAEPQTEPHDTAATAPVVAALAAEVPAETSAALVA
                     ASETPEPASTPDPTPRVHWAPEGDLPALLQRLARARETLANDPYHPGALRDELAALRA
                     LGRWQEAERSLKRLVEAEPDNLELKMEYARQLTREGYWAQAIAPLRQVVDREPDNLAA
                     WSLLAAAHQTNGHLEAARQGWTQVIELSPEDAGAYSNRGQVYVAWRDWAHAAADLEKS
                     CELDPAQEDTALALSQAYLELGRHAEATELARKVLARNPRNVRAMNRLAEALWRASSF
                     DSDEAALNEARQWWQKSLAIDPDQPAIRARLEAAGK"
     gene            complement(6784..>7193)
                     /locus_tag="PLU99_11330"
     CDS             complement(6784..>7193)
                     /locus_tag="PLU99_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO17687.1"
                     /translation="AQHIAAELLGRFRTDRAALPAMALTTDSSVLTAISNDLGYGRVF
                     VRQVEGLVQAGDVVWALSTSGNSPNVLEAVRAAAARGAVTIAFTGAGGHRLAECCAHK
                     LVVPHKSSDRIQECHQIAYHYVCGRVETAFAGK"
BASE COUNT         1249 a         2669 c         1980 g         1295 t
ORIGIN      
        1 cggtgcgcta aaacccccca aaaaacgcga gctgctgctg ggtgattaca ctccccggga
       61 gcacgccgga gcaacttgat ccgccggagc cgcgggaagt gtttcctgtc tgcgatgccg
      121 caggtgtcgc gatcctcgta cagtcaacgg aaggtccgct ttcccacctc cccacattcc
      181 cacgttccca cggaactacc agtcgcggtc caccggctta tgtcgcgccc gttcacgctg
      241 tcgcagcaat tcccgccgcg ctttctcggc atcgcgaatt ttctgcagca ggcgctcggc
      301 ctcctgccgc gacattttca cgcccggtgg cggctggtcc gccgcaggct cctcttgctg
      361 ctcagcctgc tgctcctgca agtcttcgga ctgcggttcc tcaggttgcg tggccggctg
      421 cgatgcgggc tggctttcgg gctgcggctc ctgttccccc tcctctgacg gttgcgacgt
      481 gggctgagag gacggcgatt gatcctcgtt ttcgtttgcc tggctgtctt gttcagagcc
      541 ttgttgctgc tgttcctgcc cttcctgctg gtcctgccct tcctgctgtt cggagggctg
      601 cgaggagggt tggctgtccg gctgcgagga cggctggctg gacggctgcg aagaaggctg
      661 gctggatggc tgcgactgcg gctgctgctc ttcaagctgc ttcttaagtc ggtaaaccag
      721 ctccagattc gcccgtgcat cagcgagccc gggatccaag cggattgcgt ctcggtactg
      781 acttattact cgctccagca actcctgcat tcccgtgccg cccgcccctt cttgctgcac
      841 gaggtccgcc aagtcgcagt tccccaggtt atagcgcgtg cgcgcctcga atttggcatc
      901 gcccagcggg attgctcgcg tccacagctc ccgggcctcg gccaggttgc ccagcttgaa
      961 atgcgccgcc gctcgattgt gcagcagttc cgccgcgttc cctccatccg cctcctcgtt
     1021 cgcctgctca taagcttgca gagcctcggc gaatcgcccg gctgcatagt gctcgttgcc
     1081 ttgctttatc agctccgcgg agtcgcccgc ccaggccggc gatgcaaata ccagaactac
     1141 gccggcgagc actgttctcc aatgagctaa aaagcttgaa ggacgtgtcc tgggcttcat
     1201 ccggccctcc gcttctgtcc cagtgcaatc gcgcgcatcg ccgccggtgg cgcttcgcgc
     1261 accagcgact cgattaccag tagcaacagc gccgccactg cgaacgggtg atagcgtgaa
     1321 ggccgcggaa cgcgctcgct ttcaagcttt tgttgcgcct caatcttcgg caccactacc
     1381 cggcggtaga tatcacccaa gtcgaagttc cgcgtgccca ccggcacgaa cgcgttcaaa
     1441 tccgaaaccg ccgcgatctt ccgcaaatcg tcgaaattcg cccggctcca caccgtttgg
     1501 ccgtcgtact cgagatacgt ctcacccgcc cccgccttgg caaccggtat ccgcgctcct
     1561 tcccgttcgt cacccaaggc taccgccacg atgggaatac gatgatcttc ccaaagtgcg
     1621 gccgccgccc ccaccggata gctctcgtgg tcctcaccgt cagttatcag gaaaaccacc
     1681 ttgttcgtgc tgagctgacc ggggaaacac tcgcccgcct tgcgaatggc gtcgccgatc
     1741 agcgttccgc cccgaggcga gctttcagtg gaaacgtcgc tcaacgccag gcggaaaaag
     1801 ccatagtcgt tcgtcaaagg acaggccagt gtgggtacgc ccgcaaacgt gatcaagcca
     1861 atgcgatcgc cgcccagggc cggcagcaaa tcgtcccgaa tcgacagcag cgcccgttcc
     1921 agccggttgg gtgcgatgtc gcgcgccaac atcgaacgcg acacgtccag aagcaccagc
     1981 acgtccacgt tgcgacgcac cacctgctgc tcctgctcgc cccaacgcgg attgataatg
     2041 gccgccacca acgccaccag gcacgccact atcagcccca gccgcaacag ccggcggccc
     2101 cacccgagcg gcggcgccaa acgtggcagc aagccggggg ctgcgaatcg ccgcaacgcg
     2161 cgctgccgct gccagatgcc atatacccca ataagggccg tgccgagcac cacccacagc
     2221 agccacaacc aacgctggtc aaactcgagg ttgctcatgg aatcttcctc agcaccgtcg
     2281 agtcgagcag cacctgcaga caaatgcacg cgaacgctgc cacaagccac gggcgcgaca
     2341 gttcgcccca atgcacaaac cgccgctcct ccgtcttggt ccgctccagt ctgtcgatct
     2401 cctcgtaaat cttcagcagc gagccccggt cccgagcgtg gtacgcccgc ccacccgtca
     2461 attcagccac gcgcttcagc ggctcgtcat ccaccggcat gcgggaaaag ccgcgcacgg
     2521 cgccggtgcc cgccagtatc gtgtaaacct tgatgccgta agttgccgcc agttcccctg
     2581 cttgctcggg cgatagatcc cccgcgttct gctcgccatc agtgagcagc actattaccc
     2641 ggctggtgat cgtgtactgc tgcccgctgc cggacgtgcg cttcaggtcc ttcaaccgcg
     2701 acacgcccaa ggccaacgcg tcgccgatcg ccgtcatgtc ctcttcgcga cgcgtcacaa
     2761 tttgcgtctt gtcgagcacc tcgaccagcg agctatggtc cagcgtgagc gggcagacgc
     2821 tgtccgggta cttcgcgaaa cgaatcattc cgatcagatc attcggccgc ccaggaagag
     2881 cgtcgccatc gcccagcacg aaccgccgga acacgtcctt gaccacgtcc agccgcgtca
     2941 tgggctggcc gcgcggcgac aggtccgtgt cccgcatgct gctcgaagta tcgacgacca
     3001 tctgaatcgc gatcccttcg gcaaagatct gattggactc gtccggccgt tgcggccgcg
     3061 ccaccgcgat gatcaggcag gccagagccg cgattcgcag tgccggcagg atgcgccgca
     3121 aacctgcgga ccagcgcgct cccgcggcgc gcagcggcgt caggtcggag aaccgcagcg
     3181 ccgcccgcca gctcggctgt tgccacagcc accacaacaa cggtaacaac gccagcccca
     3241 gcaacgccca aacccaggga acgggccatt gcacccactg cggcgtcagc ggcaagtggt
     3301 ggtgcgtcat gccgcctgcc cctccccgct ctgttgttgc atccgggcca gttgtgcccg
     3361 ctgcgcggcc gccgccgtct catgcacgaa tgcccgcgcc gtcatgagcg cctgatccgc
     3421 atccgtgcgg ttcggccgaa acgcggcata tttgaccagg tcgcacgctt caagaaacgt
     3481 ccgcaaacga taagcgtcat acggcagggc gctgggcttg tgagccagcg cgttgagaaa
     3541 ctcttccgtc gtcatctccg gcgccgcgag cgagaacttc cgctcgatgt acgcccgcac
     3601 gatttccgtc agttcgtagt agtagtcccg tgcccggcca atctcgatcc aatctctgcg
     3661 ccccagttgc tccaaggcct gcagcgccac caattccggc gcgaccggcg ggacgttgcg
     3721 ctgccgccgc cgaaaccata tccacgccaa gaccgccgcc gtggcaagcg cggcgatact
     3781 ggccgccagc aacagcccct tcgcaatcag ccgtttgagc gatggcttgg gcgacagcgt
     3841 gccggtgatg tcacgcgggt tcatcaacga gtcttgcgtc gtcagcgccg accggacctc
     3901 aaccttcagc gggtccgtct gcaactcgtt ctggaatcgc actggctcgc ccgcctgctc
     3961 cggcttacgg ccgtatttgg cgatcagctc cggaatctca agcgtgccgg acgccaggct
     4021 ctcaacgcga aagactcgtc gccattccag cccgccctcc ggcgccggcc gcggatccgg
     4081 cgtttcttca ccgcgaatct gcaagtcggg cagcgcctgg gcggccggaa attcgaccac
     4141 gtaatcctcc ggcgcgcgaa cttccagcac gatctcgaac ggatcgccca gccagaccgc
     4201 tttcggccgc accgtcacct caaacttcac cggcccgcgt tccttcgact cgcgtacttc
     4261 atccgattcg ggcgccggcc cgcgatcgca cccgcccagc acagccgcca ggaacacggc
     4321 tgaaacccag cacgccctga gccttatcgc acgtgaggcc ggatggcgtc cggtctgcca
     4381 gccgtgcatg atcgcccgaa gactccgcct catcgccgcc gcccccgccg atggaagaac
     4441 cgccgcaacg gctcgacgaa cgattcgccc gtcgcgacct caatcgcatc caagcgcatt
     4501 cgccgcagct ccgttatcag cgccgcatgc cgctcgtcgg ccagccgcgc gaaccgctct
     4561 cgaaaagcac gcgaactggt gtccacgagc atctgcgccc cggtttccgg atccagtacc
     4621 tcgacgtacc gcaccggcgg cagccggcgc tcccgctcgt cgcgcacgat gaccggtatc
     4681 acgtcatggc ggtgccgcac aatctgcagc ggccccgtga agctcggcgt ctgaaaatcg
     4741 ctgatcagga aaaccacggc ccgccgcgcc agcacccggc tgatggattc cagcgccacc
     4801 cccacatccg tcttccgccc cgccggctca tggtacaaca gctcacgcac cacgcgcagc
     4861 acgtgccgca tccccttgcg cggcgcgaca aaccgctcaa tccgatccgt aaataggatc
     4921 aaccccacct tgtcattgtt ctgaatggcc gagaacgcca gcgtcgcccc caattcggcc
     4981 accaattcgc tcttgagctg ctcgcgcgtc ccgaactgtt gcgatgcact cacgtccacc
     5041 gccagcagca ccgtcagctc acgttcctcc cgataattct tcacgaacgg ccgccccgac
     5101 cgcgccgtca cgttccaatc aatcgtgcgc acgtcgtccc ccggctggta ctcgcgcacc
     5161 tcctcgaact ccatcccgcg ccctttgaac gcggaatggt actgccccgc gaacacgtcg
     5221 ttgaccatcg ccgacgtgac aatctggatg cgacggacct tcttcagtat ctcagtcggg
     5281 atcatcgttt gccgccggcc gttatctgct cggcctattc ccagtccgcg accggcctaa
     5341 gtccggacag ccctcgcctg gagggtttca ttttatagac gcccaccgcc ctgaaaagcc
     5401 gtcgagaaat gctccgactt tcgatggatg cgttttgcca ggcgtcccgg cctatttccc
     5461 ggccgcctcc agccgcgccc ggatcgccgg ctggtcggga tcgatcgcca acgatttctg
     5521 ccaccactgc cgggcctcgt tcagcgccgc ctcgtccgaa tcgaagctgc ttgcccgcca
     5581 gagcgcttcc gccaagcggt tcatggcacg cacgtttcgc ggattgcgcg ccaagacctt
     5641 gcgcgcgagc tctgttgctt cggcatgccg gcccagctct aaataagctt gcgaaagcgc
     5701 cagcgccgtg tcctcctggg ccggatcgag ttcacagctc ttttccaagt cggccgccgc
     5761 atgcgcccaa tcacgccagg caacgtagac ctgcccgcga ttcgaatacg cgcccgcgtc
     5821 ctcgggcgaa agctcgatca cttgcgtcca cccctggcgc gcagcttcca gatggccatt
     5881 cgtctgatgc gccgccgcca gcaacgacca cgccgccagg ttgtccggct cccggtccac
     5941 cacctgccgc aacggggcaa tggcttgggc ccagtatccc tcccgcgtca actggcgggc
     6001 gtattccatc ttcaactcta ggttatccgg ttcggcctcg accagccttt tcaggctccg
     6061 ctcggcctcc tgccaccggc ccaaagcgcg cagcgccgcc agctcgtcgc gcaacgcgcc
     6121 gggatgatag ggatcgttcg ccagcgtctc gcgcgcccgc gccagacgtt gcagcagtgc
     6181 cggcaaatca ccctccggcg cccaatgcac gcggggcgtc ggatcaggcg tgctcgcggg
     6241 ctccggggtt tctgacgcgg ccaccagcgc cgcactggtt tccgccggga cctctgccgc
     6301 gagcgccgct acgacaggcg ccgtggcagc cgtgtcatgc ggttccgtct gcggttcagc
     6361 cgtctcatcc ggctctgcga tcgcgccggg cgttccgtcc tcttctgcgg ccgagacgtt
     6421 tgtgcttgcc tctgtcacgt ggccgacttc agcatggacc gccaccggcg cagcttgccc
     6481 ctgccaccat acccgcacca cgcgcacggc gacgattgcc accccgaccg cacacaccaa
     6541 cacgaacgcc gcagtcagct tcatgccgca atctcatccc cagcaggcgt cttcgaaaca
     6601 accgtcaccg ccattcccac tgtcgaaaac cgcacacggc aaaaaccagc ccgatcagca
     6661 tccaaaagag cgcctctgcg ttattcccgc cgacaaacag attggagtcg accatgctca
     6721 gctactccgc cgggcaggat ctcggcgcac ccaaatgctc atttcatttg ctttcggcat
     6781 tcgtcatttc cccgcgaacg ccgtttccac gcgtccgcac acatagtgat acgcgatctg
     6841 atggcattcc tggatgcggt cactggactt atgcggcaca accagcttgt gcgcgcagca
     6901 ttccgctagc cgatgcccac ccgcgccggt gaaggcgatc gtgactgccc cgcgcgccgc
     6961 cgcggcacgc accgcttcca gcacgttcgg cgagttaccg ctcgtgctca gcgcccaaac
     7021 tacatcgccc gcctgaacca agccttcaac ctggcggacg aaaacgcgcc cgtaccccag
     7081 atcattactg atggccgtca gcaccgagct gtccgtcgtg agcgccatgg ccggaagcgc
     7141 cgcgcgatcc gtacggaacc gccccaacag ctcggccgca atgtgctgcg cat
//
LOCUS       DAOSYS010000184         7122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8930_length_7122_cov_5.422669, whole genome shotgun sequence.
ACCESSION   DAOSYS010000184 DAOSYS010000000
VERSION     DAOSYS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8930_length_7122_cov_5.422669"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1216)
                     /locus_tag="PLU99_11335"
     CDS             complement(29..1216)
                     /locus_tag="PLU99_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO17688.1"
                     /translation="MRALGILLVLVSLTTGGWLGYRHWMAPPETPQYRTEEIKRGEVV
                     QTITATGTLEPVVKVLVGSQVSGTIMRWYADFNDQVKEGFVLAELDQDRFKAQVEQRT
                     AAVAVAKARVEEAQAKLSTAALERQRIEHAFARQAASDFELQSARAAEQASLAALHAA
                     EAQLQADEADLRMAAVELGKTVITSPIDGVVISRDVDAGQTVAASLSAPTLFTIANDL
                     TKMRVNAAVNETDIGIVREGMEAEFRVDAYPNRRFRGKVSQVRYAHTMVDNVVTYTTL
                     IDVDNPDLSLRPGMTATILFEVAKATDVLLVPNTALRFDPNTTTPETDWRRPGRGKPV
                     QPRVFVLDGNQLVEVPVELGLSDDKSTEIKCDALKAGDAVVVEQLNSPGMARTPRQRM
                     PRM"
     gene            complement(1999..2241)
                     /locus_tag="PLU99_11340"
     CDS             complement(1999..2241)
                     /locus_tag="PLU99_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOO17689.1"
                     /translation="MLVMRLELTDGTHGIYRLCFPFGDGNRDGRVDVADFSLMQNCWS
                     GVDIGPECDSAVFDMNADGDVDWADFALFQQLYSGG"
     gene            complement(2270..2884)
                     /locus_tag="PLU99_11345"
     CDS             complement(2270..2884)
                     /locus_tag="PLU99_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOO17690.1"
                     /translation="MPKKTDLPERLIEILQGDPTISREALSKRLQDEFNVGPSAIRKH
                     LRLLRKRGWIRPSIVVTDQAFARRPSKFWISILTGSKGVPAKDGETPLPFKPDGRLRR
                     GAGEFPERPGPGGYQIELCRDIEKNIRAHDDLFFGGIEILLGGDWDLLLWVYAYDEQA
                     VGRFVTRYLRQHPSIEKTSTAWSLMMGRSDGRLPAARTKAPAAE"
     gene            complement(3450..>4389)
                     /locus_tag="PLU99_11350"
     CDS             complement(3450..>4389)
                     /locus_tag="PLU99_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOO17691.1"
                     /translation="CGILLDERFKRHTTGHGHPERPERLDAVREGLAEAGLLETCVRI
                     EPVPLAETLIQRVHARDYLVRLDAACRQAYPYIDVPDSAIGPESYEIAKLAAGGVVKA
                     TRMVAGGELKRAFCAVRPPGHHAERDRSMGFCLLNNVALAAYALKDELQADRILILDW
                     DVHHCNGTQHTFYHDPSVLVISLHGHPNYLYPGTGWEEETGEDRGRGYTMNIPFLPAA
                     TDEMYRRAFHDRVIPAIGRFAPQFVLLSVGYDAHGDDPIGNLDLTDEAFAWMTETMIG
                     LAEAHADGRIVSVLEGGYNLGVLKRCAAEHVRLLAA"
     assembly_gap    4390..4489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4490..7107)
                     /locus_tag="PLU99_11355"
     CDS             complement(<4490..7107)
                     /locus_tag="PLU99_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17692.1"
                     /translation="MGATLLSFTSDPGALLDFVRQLQRGPARCEMNLLRRGNGVIPVH
                     VSGCRMPGQEGDRFCLVVTDLTEQKWIEELRAAEAALRDSNERLELASDVVTELLRNP
                     DPQKVVHDLARKVMHYLDCQIFLNCLLDERTGKLHLNAYEGIPDDVAERIRCPDRGAC
                     VCGAVAENGERVVVEYAAASRDPRTELVRSLGLRAYACQPLKVGERVLGTLSFGTRTR
                     DWFRLDHLGLIRTVADHIAIALERLQFQKDLCATKERLESTLSSIADAYFALDDEWRF
                     LEINHVAEQTIFKRPAEELLGKYYLEEYPMARHGEYAERLMLAAKSRVPVHFEAFSLI
                     AERWFEAHAYPYEGRLEVYSRDITLRKETELALVESQERARDEAARLQAVLDATPTII
                     WIAHDRSCQRITGSRAAYQLSRVAPGQNLSKSGPEAHKLAHFRVFKGDVELRPEEMPV
                     QRVAATGEALHDYDVDFVFNDGTRRSLLGNAVPVFDAAGQPEGAIAAFADVTEIRQWE
                     REREVLLDSERAARSEAERANQLKDEFLANLSHELRTPLTAVLGWVQLLQRGKLKAEE
                     QREAINIIDRNSRLQAQLISDLLDMSRVLSGKLRLEMQRVQIVPVVEAALASTLPLAE
                     EKRVRLDRTLDAVGPVRGDPARLQQIIWNLLSNAVKFTPPEGRVHLTVGEHNGHVEVS
                     VSDTGVGIKPEFLEHVFERFRQADGSTTRRQGGLGLGLSIVKQLTDMHGGTVHVTSDG
                     ENRGTTFTVRLPRMPALGERDAARAPAADGLPARELSDLAGTRVLIVDDDPIARDLLG
                     RILLDCKAEVMIAGSGPEALEMINASIPHVVLSDIGMPGQDGYQLLREIRKLPPERGG
                     TIPMIAVTAYARPEDRA"
BASE COUNT         1269 a         2254 c         2178 g         1321 t
ORIGIN      
        1 cgcgaatgtc gataagttcc gcgtgcattt acatcctggg catgcgttgc ctcggcgttc
       61 gcgccatgcc ggggctgttg agctgctcga ccacgaccgc gtcgccggcc ttcagggcat
      121 cgcacttgat ctccgtggat ttgtcgtcgc tcagccccag ttccaccgga acttccacca
      181 gttggtttcc atccagaaca aagacgcgtg gctgcacagg tttgccccgc ccgggccgtc
      241 tccagtctgt ttccggcgtc gtcgtattcg gatcgaatcg cagggcggta ttggggacca
      301 ataacacgtc cgtggccttg gctacctcga acaggatggt ggcggtcatg cccggccgca
      361 gtgacaggtc cggattatcg acgtcaatga gcgtcgtata cgtcaccacg ttatctacca
      421 tagtgtgcgc gtagcgcacc tgcgagacct ttccgcggaa gcggcggttg gggtaggcat
      481 ccacgcggaa ttccgcctcc atgccttcac gcacaatccc gatgtccgtc tcattgacgg
      541 cggcgttcac gcgcatcttg gtgagatcgt tggcgatcgt gaacagcgta ggcgccgaca
      601 atgaggccgc cacggtctga cccgcgtcca cgtcacgaga gatgaccacg ccgtcaatcg
      661 gcgaggtgat tacggttttg cccagctcaa cggccgccat tcgcaggtcg gcttcatcgg
      721 cctgaagctg agcctccgct gcgtgcaggg cggccagcga ggcttgttcg gctgcgcgag
      781 cgctctgcaa ctcgaaatcc gaggcggcct ggcgtgcgaa ggcgtgttcg atgcgctggc
      841 gctccaacgc ggccgtgctc aacttggcct gggcctcctc tacgcgggcc ttggccacgg
      901 cgacggccgc ggtgcgctgc tcgacctggg ctttgaaacg atcttggtcg agttcggcca
      961 gaacgaagcc ttccttgacc tgatcgttga aatcagcgta ccagcgcatg atcgtgccgc
     1021 tgacttgcga accgaccagc actttgacga ccggctcgag agttcccgtg gcagtgattg
     1081 tctgtacgac ctcgccgcgc ttgatctctt ccgtccggta ttgcggggtt tcgggcggcg
     1141 ccatccagtg gcggtatccc aaccagccgc cagtagtcag gctgaccaag accagcagga
     1201 tgccaagagc ccgcatggaa tgcattcctc cgccgccgca cgtccggcca ccgccgcgct
     1261 ttggcgacac ggatgttaac gccgtagcgg ccagctttcc aacccgcaga ccaactctgg
     1321 ccctgggcga attcgcgcgc ccgctccaat tcgccccgcc ggtcctaatc cgagcccgac
     1381 accttatcca tgcgccgcac acggatacgg agcgcagcga agcgcgacca aaaagcgaca
     1441 accgaagact gcaagtgcgt ccccgtttcg aaacgccgcc gccgcttcga aacgcgcgaa
     1501 acgccgcttt tgttcgcggc ggggaagtgg tttcatagcg ggcccggcac ttcggctgga
     1561 ttcgcagttt gccagtttgc caagtgtggt cggctatggc caataaaatc gctggtgggt
     1621 ttgcgccatg ggcaggttgc cagattgcca aggtatgcgc gctcgaattt ggcaaagtgc
     1681 cgataactcg ctgcgtgaga gcaggcaaca ggtgaaagct gaaaggagag cgggagagca
     1741 gggacggcaa gagcgagcgc attcggcgcc tccgcgcccg gagcgtgcgc atttctgcta
     1801 gtaattctag agagctttca gctttcagcc atcagccttc agccggaatt cagcggattg
     1861 tgcgtggttg cgcgccgatg gcggggagcg ggtgaaaggc acgctgcccg gaccggccgg
     1921 agcgtagcgc gcctgaaccg cgctcgaccg ctcagctttt cacacccgcc cggaacgacc
     1981 cgcgggcgca ccagtgtctc agccgcccga atagagctgt tggaacagcg cgaagtcggc
     2041 ccaatcgacg tcgccatcgg cgttcatgtc gaacacggca ctatcacatt cggggccgat
     2101 atccacgccg ctccagcagt tttgcatcaa agagaagtcg gccacatcaa cgcggccgtc
     2161 ccggttgcca tcgccgaatg ggaaacacaa gcggtaaatg ccatgcgtgc cgtcggttaa
     2221 ctcgaggcgc atcacgagca tgcccgcgtc gttgaataac gggatcgatt cactccgccg
     2281 cgggcgcctt ggttcgcgcc gccggcaggc gcccgtcgct ccgtcccatc atcaggctcc
     2341 aggccgtgct ggtcttttct atgctggggt gttgacggag gtagcgcgtg acgaaacgcc
     2401 cgaccgcctg ctcgtcatac gcgtagaccc acagcaacag gtcccagtcg ccgccgagca
     2461 ggatttcgat cccgccgaag aacaaatcgt catgggcacg gatgttcttc tcaatgtcac
     2521 ggcacagctc gatctggtaa ccgcccggcc caggccgctc aggaaactct ccggccccgc
     2581 gccggagccg cccgtccggc ttgaacggca gcggggtttc gccgtccttg gcggggacgc
     2641 ccttggagcc ggtcaaaatc gaaatccaaa atttcgacgg acgccgcgcg aacgcctggt
     2701 ccgttacgac aatcgacggc cgaatccagc cgcgcttccg cagcagccgc aggtgcttgc
     2761 gaatcgccga ggggccgacg ttgaactcgt cctgaaggcg ttttgagagc gcctcgcgcg
     2821 aaatggtcgg atctccctgg aggatttcga tcaaccgttc gggcaaatca gttttttttg
     2881 gcatagactg acccttttta tcggccggag aaaacgcgaa ggcgccacta ttccgatagc
     2941 tatgatgcgc ggtaggcgtc tatccgccgt gtttagcgcg tttgagtaac atattggaac
     3001 attcttggcc tggtaaagtc aagcgccgca cggtcaccga aggctaccta aggcgccggc
     3061 gcatggtgtc ggataagtcg caaaatgaaa ttttcgtaca aaaacgcgct aactacttgc
     3121 caagaccgtt tcactgtggt aatctgatat atgagtgagg tgggaacgcg atgagttttt
     3181 ccactgagct ccgtgcctcg gacgcaagaa gtcggaattg aaagctaagc gtcagaggtt
     3241 tacgacataa aatagagaga cacgcggctc gccgcgttgc acgaacctcc tatgttaagt
     3301 gccttcactc cttcgcctcg agggcgccac acgccctcga ggccgatttt gtatccacgc
     3361 aaaaactgaa cgttttctcg cagcgcttca gcgcgtgaaa cgcccagaaa gcgccgctca
     3421 tgcaacccgc cgggtcaagt cgtcccgctt cacgccgcca gcagccgcac gtgctcggcc
     3481 gcgcagcgct tcaaaacacc caggttgtac ccgccctcga gaacgctgac gatgcggcca
     3541 tcggcgtgtg cctcagccag gccgatcatc gtctcggtca tccacgcgaa cgcttcatcg
     3601 gtgaggtcca ggttcccaat cggatcgtcg ccatgcgcat cgtagccgac tgagagcaac
     3661 acgaactgcg gcgcgaaccg cccgatggcc ggtatgaccc gatcgtggaa ggcccggcga
     3721 tacatttcat cggtggccgc gggcaagaag ggaatgttca tcgtgtagcc gcggccacgg
     3781 tcctcgccgg tctcttcctc ccatccggtc cccggataca ggtagttcgg atgaccgtgc
     3841 agacttatga ccagcacgga cgggtcgtgg tagaaggtgt gctgcgttcc gttgcagtgg
     3901 tgcacgtccc agtcgagtat cagtattctg tccgcctgaa gctcgtcttt gagcgcgtac
     3961 gcggccagcg cgacattgtt cagcaagcag aaccccatcg aacggtcgcg ctcggcgtga
     4021 tggcccggcg ggcgaacggc gcaaaaggcc cgcttcagtt cgccgcccgc caccatgcgc
     4081 gtcgccttga ctactccgcc cgccgcaagc ttggcaatct catagctctc cgggccgatc
     4141 gcactgtccg gcacgtcgat gtagggatag gcttggcgac aggcggcgtc cagccggacc
     4201 aggtaatcgc gcgcatgcac gcgctgaatc agcgtctccg ccagcgggac gggctcaatg
     4261 cgcacgcagg tttccagcag tcccgcctcg gccaaccctt cccgaaccgc gtccaggcgc
     4321 tcgggacgct ccgggtgtcc atggccggtg gtatgtcgtt tgaaacgctc gtcgagaagt
     4381 atgccgcagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cccggtcttc
     4501 gggacgcgcg taggccgtga ctgcgatcat gggaatcgtc ccgccgcgct ccgggggcag
     4561 cttgcgaatc tcgcgcagaa gctgataacc atcctgcccg ggcatgccga tgtcgctcag
     4621 cacgacgtgc ggaatcgagg cgttgatcat ctccagcgct tcgggcccgg agcccgcgat
     4681 cattacctcg gctttgcagt ccagcaaaat gcggccgagc aggtcgcgcg cgatcgggtc
     4741 gtcatccaca atcaggacgc gcgtgccggc caaatcagac agttcgcgcg ccggcagacc
     4801 gtccgcggcc ggagcgcgcg ccgcgtcgcg ctcgcccagg gcgggcatgc gcggcaggcg
     4861 cacggtaaac gtggtgcccc gattttcgcc gtcgctggtg acgtgcaccg ttccgccgtg
     4921 catgtccgta agctgcttca caatcgacag cccgagcccc agcccgccct ggcgacgcgt
     4981 ggtcgaaccg tccgcctggc ggaaacgctc gaagacgtgc tccaggaact ccggcttgat
     5041 gccgacgccc gtatccgaga ccgatacctc gacgtggcca ttgtgctcgc cgaccgtcag
     5101 gtgcacgcgg ccttcggggg gtgtgaactt gaccgcgttt gacagcaggt tccagatgat
     5161 ctgctgcaag cgcgccgggt cgccgcgcac cgggcccacc gcgtccagcg tacgatcgag
     5221 ccgcacgcgc ttctcctccg ccagcggcag ggtcgaggcc aacgccgctt cgacgaccgg
     5281 gacaatctgc acgcgctgca tttccagccg cagcttgccg gagagcacgc ggctcatgtc
     5341 cagcaggtcg gagatgagct gggcctgcaa gcgcgagtta cggtcgatga tgttaatggc
     5401 ctcgcgctgc tcttccgcct tgagtttgcc gcgttgcagg agctgcaccc agcccagcac
     5461 cgcggtcagc ggggtccgca gctcgtgcga gagattggcc agaaactcgt ctttgagctg
     5521 attggcgcgt tccgcctcgc tgcgtgcggc gcgctcgctg tccagcagca cttcacgctc
     5581 gcgttcccac tgccggattt cggtaacgtc cgcgaaggcc gcgatcgcgc cttccggctg
     5641 gccggcggca tcgaagacgg gcacggcgtt gcccagcaac gagcgccgcg ttccgtcgtt
     5701 aaacacgaag tccacgtcgt aatcgtgcag cgcctcgccc gtggcggcca cacgctgaac
     5761 cggcatttct tccggccgaa gctccacgtc gcccttgaag acgcgaaagt gtgccagttt
     5821 gtgcgcctca gggccggatt tggacaagtt ttggcccggc gcaacgcgcg agagctggta
     5881 ggccgcacgg ctcccggtaa ttcgctgaca gcttcggtcg tgggcaatcc aaatgatggt
     5941 gggggtagcg tccaggacgg cctgcagccg ggcggcctca tcgcgggcgc gctcctggct
     6001 ttcgaccagg gccagttctg tttccttgcg cagcgtgatg tcgcgcgagt acacctcgag
     6061 ccggccttca tatgggtagg cgtgggcttc gaaccagcgt tcggctatca gcgagaaggc
     6121 ttcgaagtgt accggcaccc tggactttgc ggccagcatc agccgttcgg cgtattcgcc
     6181 gtgccgcgcc atgggatact cttccaggta gtacttgccc agcaactcct cggccgggcg
     6241 cttgaatatg gtttgctcgg cgacatggtt gatctcgaga aagcgccatt cgtcgtccag
     6301 agcgaagtag gcatcggcaa tgctgctcag ggtcgattcc aatcgctcct tggtggcgca
     6361 gaggtccttc tgaaattgaa ggcgctccag cgctatggcg atgtggtcgg cgaccgttcg
     6421 aatcaggccc aggtgatcga gtctgaacca gtcgcgcgtg cgcgtgccga acgagagcgt
     6481 gcccaaaacg cgctcgccca ccttgagcgg ctgacaagcg taggcgcgga gtccgaggga
     6541 acgcacgagt tctgtgcgcg ggtcgcggct ggcggcggcg tactccacga ccacgcgctc
     6601 cccgttttcg gccacagcgc cgcacacgca tgcgccacga tcgggacacc ggattcgttc
     6661 ggcgacgtca tcggggatgc cttcataggc gttgaggtgc aatttgccgg tgcgttcatc
     6721 gagcagacaa ttcaagaaga tttgacaatc caggtagtgc atcactttgc gggccaggtc
     6781 gtgaacgacc ttctgcggat cggggttgcg cagcagttcc gtcacgacgt ccgatgccag
     6841 ttcgaggcgc tcattgctgt cgcgcaaagc ggcttcggcg gcgcgcagct cctcgatcca
     6901 cttctgctcg gtcaagtcgg tgaccaccag gcaaaagcgg tcgccctcct gtcccggcat
     6961 gcggcagccg gaaacgtgga ccgggatcac tccattgccg cgccgcagca ggttcatttc
     7021 gcagcgtgcc gggccgcgct ggagctggcg cacgaaatcc agcagggcgc ccggatcgga
     7081 ggtgaagctc aacaaggtcg cccccattac ttgctccagg gg
//
LOCUS       DAOSYS010000185         7110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8943_length_7110_cov_5.241956, whole genome shotgun sequence.
ACCESSION   DAOSYS010000185 DAOSYS010000000
VERSION     DAOSYS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8943_length_7110_cov_5.241956"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2499
                     /locus_tag="PLU99_11360"
     CDS             <1..2499
                     /locus_tag="PLU99_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17693.1"
                     /translation="VIEQNSDGQLTLAQAFEPRSPTPSTPKPAAELPAPEGRLIVKNG
                     AVKVVRPGAAPYDMTELDGQVDLKTLNEVGARFALTLADGTKLAGEAAVRRLLTQGKL
                     DPRNASGDLRLSSDRNVQIGPLAALAGQPGLQGGLNVDVDAKLDSGTLDGRFTIAVAG
                     LQSEQRAAANATPINAELKGQVRWADNQLAANLNLAGEAGQAQAELAYRGSDQPSNFT
                     VDELVTAALSGKPVAVPDFTMRAQAEIDLAKLGRAVPELLNVKEGVELTAGRLELADL
                     TIQGGAEPKASAKLSLKDVAARGSDKPIRLDPVQFSLDSALESGRGLQVRGVELTASS
                     MQLKASGPANDLHAAFKADLGRLQQELGQVFDLGALELAGNTSGTVDFKRVNDVQIDV
                     GIRATVEGLRYASAGRRLDGAVQAQTSIRPAAEARPLTLGGEWLVQNARLDGKALADG
                     DVKLTYDVKLDQDKQSITIDKCSLASKALTAEIAGTIAQYTGDCQLNLQGRYSASWEA
                     VTALLHKLTPATADTVIIAGNSASEFKITGPACAKTQPGFRDMSTGAGVTWTSAELYG
                     VRMGAAQLAPALKDGRLTLPKATIPAGDGKVNLGAVIDFQPADPTLQMPGKTKLLENV
                     PVTKELGAQLLSRINPIFLHVANIEGRVNLGLQDVSVPLGDSIKQRGTAQGRLDLSKM
                     KMQPGGLAAELLALGGMDGSRAYPVEVGALDFVMKDGRIRYDDFTLVFPGDFDLKFRG
                     SVGLDDTLELVVSIPLRAPLLDRLGAKGPTQEYAERLRGTRVELPILGTREKPRLDFS
                     KVDVQALLKDVLLNEPAKQLEGFLKGLGGQDKKP"
     gene            2557..3999
                     /locus_tag="PLU99_11365"
     CDS             2557..3999
                     /locus_tag="PLU99_11365"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOO17694.1"
                     /translation="MSASSRKRLENFFETPDQTLSRWARLVGFQFALWRFCARRLREH
                     DVMGMSAALCYRTIFTLIPVLVLALLVAKSLGTVEDSKQSLRKFLKASGFAEIAAVQD
                     GEEAGLAGPEAIAAAAPESQAAESSPRKVINVADEIERIVTGVEGKLTFARIGPIGAV
                     LLIWTAVGLLSSLEQSLNRIFGAARSRSVARRLLLYWSVLTLGPITLAAASYLGQKAI
                     DMFKLGSGLPWLLVAVGWAGPIVVAVLVLAAVYKLLPNTSVRWASAIGGAAVATVLWL
                     VAKWAFGLYVDRLVLKGNLYGVLGVLPLFLLWLYCSWLILLFGAELAHTAVNVEGMRR
                     LELAERTVLGPSDVLAAAIAVAQPYEEGRGPVTLDEVAARLRLPGANVQTLLDRLAGA
                     GLVCTTEDSAAPRYLPARPPAQIPMLALLDLGDPRAVHGGYGPELAAAISYVQSHIRG
                     GLTDSTLADALEAAQAESAPWVASGRAVGG"
     gene            complement(4208..6019)
                     /locus_tag="PLU99_11370"
     CDS             complement(4208..6019)
                     /locus_tag="PLU99_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOO17695.1"
                     /translation="MTDPTLLRRIQVTDEELDREFRQALGESYSPEAIDKQLGNAVHD
                     FTPNAIVKARVVNIADREVILDIGLKSEGIVSLDEWDDPSQIDVGDEIEVLVEEVESE
                     TGMVTVSKRRADRILNWKRIVETTHEGDTVKGRCLRKIKGGLLVDIGVPVFLPASQVD
                     IRRPADIGEFIDKDIEAKILKIDEERRNIVISRRKLIEEERQRAKERLMSEIAVGQIR
                     KGTVKNIADFGAFVDLGGIDGLLHITDMSWDRIEHPSQMLKIDEEIEVKILNIDRDKE
                     KIALGLKQKEENPWERVAERYPVGGRVRGEVVNIMNYGAFVKLEPGVEGLVHISEMSW
                     TRRINHPSEVVALGDQIEVVILEVNKEKQEISLGMKQTEINPWTTVAQKYPPGTVVEG
                     AVRNLTNYGAFVEIEEGIDGLLHISDMSWTKKVSHPSEMVKKGDRLRCVVLSVDQEKM
                     RVALGLKQMTEDPWMRAVPDRYQPGMVIRGKVTKITNFGVFVELEPDLEGLLHVSELA
                     DHKVESPQDVVKIGDEIDVKILRVDTIERKIGLSKKRADWAGEAGHEEEAPRKERKRR
                     GGLHGPGEGFLGEIDDVLFHKPQQEPPPPPPPAPEQP"
     gene            6352..>7110
                     /locus_tag="PLU99_11375"
     CDS             6352..>7110
                     /locus_tag="PLU99_11375"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOO17696.1"
                     /translation="MKKLATEEVSLARRLMPYGTHAAAALGGAAAVLVVLALSGVFKH
                     SDPVPPLPPPPDPVTTPPAAYDWTAHPDPEFPLPPYACYLQDVLIVLDPGHGGRSDRP
                     NWKRGPTGLEEAVVNLRVAQFLREFLTEVGAQVVMTREDDVYLDPDIAVDNRKRIELA
                     NRLKADLFVSIHHNGDDEPDANYTTVFYHGGPDDSPASRCAARFLLSGINDALRLTQH
                     LPCAMLSDRVLFKTGLLVLREARVPAVLVEASFHS"
BASE COUNT         1215 a         2193 c         2343 g         1359 t
ORIGIN      
        1 gttatcgaac agaacagcga tgggcaactc acgcttgcgc aggcctttga gccacgctcg
       61 ccgacgccct caacgccgaa gccggccgcc gaactgccag cgccggaggg ccgcctgatt
      121 gtgaaaaacg gcgccgtcaa agtggtgcgc ccgggagcag caccatacga catgaccgag
      181 ctcgatggcc aagtggatct caagacgctg aatgaagtcg gcgccagatt cgcgctcacg
      241 ttggcggatg gcaccaaact cgcaggcgag gcggcggttc gacgattgct cactcagggc
      301 aagctggacc cgcggaatgc aagcggcgat ctccgcctca gctcggaccg caacgtgcaa
      361 atcggaccgc tggccgccct ggcaggccag cccggccttc aaggcggact caacgtcgat
      421 gtcgatgcca agctcgactc gggcacgctg gacggccgct tcactatcgc cgtggctggg
      481 ctgcaaagcg agcaacgggc ggcggccaat gcgacgccca taaacgcgga gctgaaaggg
      541 caggttcgct gggcggacaa ccagcttgcc gccaatctca acctggccgg tgaggccgga
      601 caggcgcagg ccgaacttgc gtatcgaggc tccgatcagc cgtcgaactt tacggtggat
      661 gagcttgtca ccgcggcgct gagcgggaag cccgtggcgg ttcctgattt cacgatgcgc
      721 gcgcaagccg aaatcgacct ggccaagctc ggccgggctg tcccggaatt attgaacgtc
      781 aaagaaggcg tggaactgac ggccgggagg ctggagctgg cggatttgac gattcagggc
      841 ggcgcggaac ccaaagcgag cgccaagctg tcgctgaaag acgtggcggc gcgcggcagt
      901 gacaaaccca tccggctcga cccggtgcaa ttcagcttgg actcggctct ggagagcggc
      961 agggggcttc aagtgcgggg cgtggagttg acggccagct ctatgcaact gaaagccagc
     1021 gggccggcca acgatttgca cgccgcgttc aaggccgact tgggccggct gcagcaagaa
     1081 ctcggccagg tattcgacct gggtgcgctt gagctggcag gtaacaccag cggaactgtc
     1141 gacttcaaac gggtcaacga tgtacaaatc gatgtcggca ttcgggcaac ggtggaaggc
     1201 ctgcgttacg ccagcgccgg gcgccgtctg gacggtgcag tgcaggcgca gaccagcatc
     1261 aggccggccg ccgaggcgcg gccgctgacg ctgggcggcg agtggctggt tcagaatgcc
     1321 cgcctggacg gcaaggccct ggcggacggc gatgtaaagc ttacctacga cgtgaagctc
     1381 gaccaggaca agcaatccat aacaatcgac aagtgcagcc tggcttcgaa agcgttgacc
     1441 gccgaaatcg ccggcaccat cgcgcagtac accggagatt gccagttgaa tctgcaaggg
     1501 cggtattccg cgtcctggga ggcggtgact gctctactgc acaagctgac gccggccact
     1561 gcagacaccg tcatcatcgc gggcaacagc gccagtgaat tcaagatcac cgggccggcg
     1621 tgcgccaaaa cccagcccgg tttccgcgac atgagcactg gagcgggcgt gacgtggacc
     1681 tcggccgagt tatacggcgt cagaatgggc gcggcgcagc tcgcgcccgc gctgaaggat
     1741 ggccgtctga cgctgcccaa ggccacgatt ccggccggcg acggcaaggt taaccttggt
     1801 gccgtgatcg atttccagcc cgcggatccg actcttcaga tgccgggcaa aaccaagctg
     1861 ctggagaacg tgccggtcac gaaggaattg ggcgcccagc tactgagccg aatcaacccc
     1921 atattcctgc acgtcgcaaa cattgagggc cgcgtgaacc tgggcttgca ggacgtttcc
     1981 gtgccgctcg gcgactcgat caagcagcgc ggcacggccc aagggcggct ggatctcagc
     2041 aagatgaaga tgcaaccggg cggtttggcc gccgagctgc tggcgttggg cggcatggac
     2101 gggagcaggg cctatcccgt ggaggtcggc gcgctcgatt tcgtgatgaa ggacggacgc
     2161 atccggtacg atgatttcac gctggtcttt cccggcgact tcgatctgaa gttccgcggc
     2221 tcagtcggcc tggatgatac gctcgaacta gtggtgtcga tcccattgcg ggcgccgctg
     2281 ctcgaccggc tgggcgccaa gggaccgacg caagagtatg ccgagaggct gcgcggcacg
     2341 cgtgtggaac ttccgatcct gggcacgcgc gagaagccgc ggctggactt ttccaaggtg
     2401 gacgtgcagg cgctgctcaa ggatgttctg ctgaatgagc ccgccaagca gcttgaaggt
     2461 tttctgaagg gcttgggcgg tcaggacaag aagccctgac cgttcgcgtc aagctgccac
     2521 gcagcgctgg aaggccgcgg cacgcgagga ggagtcatga gcgccagttc ccggaagcgg
     2581 ctggagaact ttttcgagac gccggatcag acgctcagcc gctgggcgcg cctggtcggc
     2641 tttcagttcg ctctgtggcg cttttgtgcc cggcggctgc gcgagcacga tgtcatgggg
     2701 atgagcgccg cgctgtgcta ccgcacgatc ttcacgttga taccggtact ggtgctggct
     2761 ttgcttgtcg ccaagtcact ggggaccgtc gaggacagca agcagagtct gcggaagttc
     2821 ctcaaagctt ctggtttcgc cgaaatcgcg gccgtgcagg acggcgagga ggcagggctc
     2881 gcggggccgg aagccatagc cgcggcggcg cccgagagcc aggccgccga gtcgagcccg
     2941 cgcaaggtca tcaatgtggc ggacgagatc gaacgcatcg ttaccggcgt cgagggcaag
     3001 cttaccttcg ctcgcattgg cccgatcggg gcagtactgt tgatttggac ggcggtgggt
     3061 ttgctctcct cgctggagca atcgctcaac cgcatctttg gcgcggcccg cagccggtca
     3121 gtggcgcgtc gcctgctgtt gtattggtca gtgctcacgt tgggaccgat cacgctcgca
     3181 gccgcgtcct acctgggaca gaaggcaatt gacatgttca agcttggctc cgggctgccg
     3241 tggttgctgg tcgcggtcgg ctgggccggg ccgatcgtcg tggcagtgct cgtgctggcc
     3301 gctgtatata agctgctgcc taatacgagc gttcgctggg catcggccat tggcggggcg
     3361 gcggtcgcaa ccgtgctatg gctggtggcc aaatgggcct tcggcttata cgtcgaccgg
     3421 ctcgtgctga aaggaaacct gtacggcgtc ctgggcgtgc tgccgctatt cctgctgtgg
     3481 ctctactgct cgtggctgat actcttgttc ggcgctgagt tggcgcatac ggccgtgaac
     3541 gttgaaggca tgcggcgctt ggagctggcg gagcgcaccg tgcttggacc gtcggacgtg
     3601 ctggccgcgg ccattgcggt cgcgcagcca tacgaggaag ggcgcgggcc ggtcacactc
     3661 gacgaggttg cggcccgcct gcgtctgccg ggtgcgaacg tacagacgct gctggaccgc
     3721 ttggcgggcg cggggctggt gtgcacgact gaagactcgg ccgcgccgcg ttatcttccg
     3781 gcccgtccgc cggcgcagat tcccatgctc gcgctactgg atttgggtga tccgcgcgct
     3841 gtccatggcg ggtacgggcc ggagctggcg gccgccattt cgtacgtgca gtcacatatc
     3901 cgcggcggct tgactgactc cacactggcc gatgctctgg aagcggcgca ggccgaaagc
     3961 gcgccctggg ttgcgtccgg cagggcggtc ggcggatagt tggccgcgac tggcggctca
     4021 taagctcgtc taccaattta gttggttcgt cacgttattg cggcgagtat tgggccgtag
     4081 cgcctgcgga ggcgaagagc ttgaatgata agggccgcgc gttttttgac gcgcggcccg
     4141 ttctggttac gtcatcagtg gtgcactgct caagagcagt ggcacgctag tgtgcacacc
     4201 gcacttctta tggctgctcc ggtgcgggcg gcggtggcgg cggcggttcc tgctggggct
     4261 tgtggaacag cacgtcgtcg atttcgccca ggaaaccttc gcccggcccg tgcaggccgc
     4321 cgcggcgctt gcgctctttg cgcggggctt cctcttcgtg cccggcttcg ccggcccaat
     4381 cggcgcgctt cttcgaaaga ccgattttgc gctcgatcgt gtccacgcgc agaatcttga
     4441 cgtcgatttc gtcgccgatc ttgaccacgt cctgcgggct ctcgaccttg tgatcggcca
     4501 gctcggaaac gtgcaacaag ccctcgaggt cgggctccaa ctccacgaag acgccgaagt
     4561 tggtgatctt ggtgaccttg ccgcgaatga ccatgccggg ctggtaacga tccgggacgg
     4621 cccgcatcca cgggtcctcg gtcatctgct tgaggcccag ggcgacgcgc atcttctcct
     4681 gatccacgct cagcacgacg caccgcaggc gatcgccctt cttgaccatc tccgacgggt
     4741 ggctgacctt cttggtccag ctcatgtcgg aaatgtgcag caggccgtcg atgccttcct
     4801 cgatctcgac gaacgcgccg tagttcgtga ggttgcggac ggcgccctcg accaccgtgc
     4861 ccggcgggta cttctgggcg accgtggtcc aggggttgat ctcggtctgc ttcatgccga
     4921 gcgagatttc ctgcttttcc ttgttcactt cgaggatgac cacctcgatc tgatcgccca
     4981 gcgcgaccac ctcggacgga tgattgatgc ggcgcgtcca gctcatctcg gagatgtgga
     5041 ccaaaccttc gacgcccggc tccagcttca cgaacgcccc gtaattcatg atgttgacga
     5101 cttcgcccct tacgcggccg ccgaccgggt aacgctcggc cacgcgctcc caggggttct
     5161 cttccttctg cttgaggccg agggcgattt tttccttgtc gcggtcgatg ttgaggattt
     5221 tgacctcgat ttcctcgtcg atcttgagca tctggctggg gtgctcgatg cggtcccacg
     5281 acatgtcggt tatgtgcagc aagccgtcga tgccgccgag gtcgacgaac gcgccgaaat
     5341 cggcaatgtt cttgacggtg cctttgcgga tttgtccgac ggcgatttcg gacatgagcc
     5401 gctctttggc acgctggcgt tcttcttcaa tcagcttgcg gcgcgagata acgatgttgc
     5461 gccgctcctc gtcgatcttg aggatcttgg cctcgatgtc tttgtcgatg aactcgccga
     5521 tgtcggccgg ccggcggatg tccacctgcg aggcgggcag gaagaccggc acgccaatgt
     5581 cgaccagcag gccgcccttg atcttgcgca ggcaacggcc cttgacggtg tcgccttcgt
     5641 gagtggtttc gacgatgcgc ttccagttaa ggatgcggtc ggcgcggcgc ttggaaaccg
     5701 tcaccatgcc ggtttcggac tcgacttctt cgacgagcac ttcgatttca tcgccgacgt
     5761 cgatctggga aggatcatcc cactcgtcca ggctcacgat gccttcgctc ttgaggccga
     5821 tgtcgaggat cacttcgcgg tcggcgatgt tgaccacgcg cgccttgacg atggcgttcg
     5881 gagtgaaatc gtgaacggcg ttgccgagtt gcttgtctat ggcctcaggg ctgtagcttt
     5941 cgccaagggc ctgccggaac tcgcgatcga gttcctcatc agttacttga attctcctaa
     6001 gaagagttgg gtcagtcata caatggttcc gaatttggcc gcgccatccg gccagccccg
     6061 ttacggcacg cggcgttcgc agggaggggt tgggctaggc gactatccgc cccgcgcgcc
     6121 ggaacacccg gacgcttcct ggggggcgga gtgactaact atacaaggcc gtccgacgct
     6181 cggcaatgcc cgtcgccgag gaaatcatgc gtccccagtg cgttgaaacc tcgcggttgt
     6241 cgcctcgtta ggccgcgcct tacaatttct tccggatggg aatcaccgga tcaccggccg
     6301 gccggctacg ggctggccga cagcgtgcgg aaaacctggt gcattgcggc cttgaagaag
     6361 ctcgccacgg aagaagtcag tttggcacgg aggctgatgc cctatggcac gcacgccgcc
     6421 gctgcgctgg ggggcgccgc cgctgtcctg gtcgtgctgg cgctcagcgg cgttttcaag
     6481 cactccgatc cagttccgcc gctaccgccg ccaccggacc cggtcactac cccgcccgct
     6541 gcgtacgact ggacggcgca tcctgacccc gaattcccgc tgccgcccta cgcctgctat
     6601 ctccaagacg tcctgatcgt gctcgatccc gggcatggcg ggcgttctga tcggccgaac
     6661 tggaagcgcg gcccgaccgg cctggaggaa gcggtcgtca acctgcgcgt ggcgcagttc
     6721 ctgcgcgaat tcctgaccga agtgggggca caggtagtga tgacgcgcga ggacgacgtt
     6781 tacctcgatc ccgacatcgc ggtcgacaac cggaagcgta ttgagcttgc gaaccggctc
     6841 aaggcagacc tgttcgtgtc aattcaccac aatggcgacg acgagcccga cgccaactac
     6901 acgaccgttt tctatcatgg cgggcccgac gacagccccg ccagtcgctg tgccgcacgc
     6961 ttcctcttga gcggcattaa tgacgccctg cggctgactc agcacctgcc ctgcgccatg
     7021 ttgagcgatc gcgtgttgtt caagaccggc ctgctggtgc tccgcgaagc gcgcgtgccg
     7081 gcggtgctgg ttgaggcctc ctttcacagc
//
LOCUS       DAOSYS010000186         7103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8958_length_7103_cov_5.004824, whole genome shotgun sequence.
ACCESSION   DAOSYS010000186 DAOSYS010000000
VERSION     DAOSYS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8958_length_7103_cov_5.004824"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1348
                     /locus_tag="PLU99_11380"
     CDS             386..1348
                     /locus_tag="PLU99_11380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOO17697.1"
                     /translation="MENERNGRVTVVIPTYNRAHYLPGAIRSVLNQTAADRCDIVVVD
                     DGSSDDTPGVVAQFGNHVRYLRQPNAGSAAARNAAINASDAEFVAFLDVDDEWLPDKI
                     ERQLAVMTRCPEAALVAGRSYDRASGAPLRLRPEPPVATDAPVDFAPALFKDNFIPTP
                     VVMVRRSALEHVGLFQPELRQAQDYHLWTRLACHGRGVYLSAPLAVITTDDPDSVSRD
                     AARLLRWSLRARQLLRRELRVRPDCRPAWRRGMAACLAQLRDECYRRREFADATRFGA
                     ASLMYRPWPRPKWEFGRLLASAARAAVEPVRKARHSSQVQCSAT"
     gene            complement(1363..2478)
                     /locus_tag="PLU99_11385"
                     /pseudo
     CDS             complement(1363..2478)
                     /locus_tag="PLU99_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2529..3704)
                     /locus_tag="PLU99_11390"
     CDS             complement(2529..3704)
                     /locus_tag="PLU99_11390"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOO17698.1"
                     /translation="MAFSYVVLGAGRQGVALAYDLAKNCEAGRVRLADVSPAQVDSAL
                     ARLRPLLPAMATQFEAVICDVSRPVEVAHALAGADVVISAAPYRFNAELTEAAIAAGA
                     SFCDLGGNTTVVRQQLAQHAAAEARGVSVVPDCGLAPGLANILAAHGVSQMDAPRDVH
                     VRCGGLPQQPVGPLGYKLVFNFDGLVNEYSGFGEFLRDGRRAAVPALTELEEIEFAPP
                     LGKCEAAVTSGGTSTCAASFAGRLRTYDYKTVRYPGHFAVVRAMFELGLFEERVTLSD
                     GQVIAPKPVLRQLMEDRLRFPEVRDLVVLRCTVSGTHRGAPRTLQYDLFDRHDEQTGF
                     TAMERTTAFPASLVAYMQARRLLAPGARPLELCLPTEQYMAELAQHDIHVVVQDIAG"
     gene            complement(3732..4295)
                     /locus_tag="PLU99_11395"
     CDS             complement(3732..4295)
                     /locus_tag="PLU99_11395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOO17699.1"
                     /translation="MARITRVYTRTGDDGTTGLGSGRRVPKNALRIEAYGTIDELNAH
                     IGTVLAAGPVPELVEPLRRIQNELFHLGADLSIPEADKPKHPSPRLSAQHVTALENCL
                     SSLNQDLPALKNFVLPGGTPVAAFLHVARTVCRRAERIVVALAAQEPVGADNVPYLNR
                     LSDLLFVMARFHNQKCGVSDPIWNSRT"
     gene            4500..4940
                     /locus_tag="PLU99_11400"
     CDS             4500..4940
                     /locus_tag="PLU99_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17700.1"
                     /translation="MDNWKQTFLDKLTQAQSQWISRFDEALNTHFVPVFEEYRQFLAD
                     NGFRLSVPMREPGRRSFKFELSENAYLLMILRAMGVGELELRCESFVPGCDPAHSRSV
                     GLVSQVTQEWVTERVRGALETFVDLLSKAVPPEVSEPLEELVSA"
     gene            complement(4990..5940)
                     /locus_tag="PLU99_11405"
     CDS             complement(4990..5940)
                     /locus_tag="PLU99_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOO17701.1"
                     /translation="MSGVRVSAVRAIQFALLMAATLLAGCSAFQANPFEIQPTFRDLA
                     YGDHPRQKLDLYLPHDAGGPRPVIVWIHGGRWSGGEKYPAYPAELLTEAGFAVASIGY
                     RLSGEAAFPAQINDCKGAIRWLRAHAGEYALDADRIGVWGRSAGGHLAALVGTSGDVA
                     ELEGDVGGNLDCSSRVQAVADYYGPTNAFTFAAHVDDCGSGEPPLLGRCLGDIVAHAD
                     DPEWAQWVELARLASPVYSVTPDDPPFFIAHGTADTRCPPSQSQELYGRLTAAGVQVT
                     LSLVAGAEHGQLPAAEDVKVVDFFRATLGEAAGEQKQYQL"
     gene            complement(6008..6178)
                     /locus_tag="PLU99_11410"
     CDS             complement(6008..6178)
                     /locus_tag="PLU99_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17702.1"
                     /translation="MRPTRAAILVAWGALIVPSWFVGSEWAWEDELKYLGAILAALLV
                     LMVRALRRERQM"
     gene            complement(6175..>7103)
                     /locus_tag="PLU99_11415"
     CDS             complement(6175..>7103)
                     /locus_tag="PLU99_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17703.1"
                     /translation="PRKCRQRLLGRMLVVAGTALVLTLPLIVHMVAVHAQNPRLLQYM
                     AAEVTRPPFYAHAYTPLVVPAFLVGVWVILRHAPQTFWAVAYLLAALLLQIPAYLARY
                     LGLNAPYLLPHEAQWHMQIAEGLCAAVAIMWLARQLAQAARRPRLVPAAAIVLGVLAV
                     GPAMWHLAPDDSYFVSVAQLQEQTRQVRAWIQTHTALEDTIACDPELGYKVVSGLTGR
                     KCIAVPIGQMNPAPDVRQRHDVLHALVSGKEPAAAAETARKYGARYLLFQTTEGGAAT
                     IERWDACPALERCFTADDGSAVIYRIRAEESP"
BASE COUNT         1323 a         2441 c         2154 g         1185 t
ORIGIN      
        1 gaagtagagc agctcgcggt aacgccacag ttcgccgacg tcgatcgcgc gccagcccgg
       61 cgtcggccgt attaccagca gcggtccgtc ggccgccgcc aatgcggcgt ttatcgcggt
      121 ggtctggctc acggcgcgcg tcaacgagaa ctccctggcc ggcaaatacg ttcgcggtga
      181 acgcactgaa cccccagccc gtatcggacc gtgcttgcgg gcgctttagc catgggcatc
      241 gcgcaaaacg ccctccctgg cggccctgga aacggctgga ccacagaaac gactgctcac
      301 ataaagccag gccgcgccac gcccgatatc ccggtcggtc tggccctgtc ccggtcggcg
      361 gcaaaccgtt gttgcggcaa agattatgga aaacgagcga aatggccgtg tcacagtcgt
      421 catccccact tataaccggg cgcactatct gcctggggcc atacgcagcg tgctgaacca
      481 gaccgcggcc gaccgctgcg acatagtcgt ggtcgacgac ggcagctcgg acgatacgcc
      541 cggcgtcgtc gcgcaattcg gaaaccacgt gcgatatctg cgccagccca acgccggctc
      601 cgccgcagcg cgcaacgcgg ctatcaacgc ctctgatgcg gagttcgtcg cgttcctgga
      661 cgtcgatgac gaatggctgc cggacaaaat cgagcgacaa ctcgcggtca tgacgcgttg
      721 tccggaggca gcgctggtag ccggccgctc gtatgaccgc gcctccggcg ctccgctgcg
      781 gctgcgtccg gagccgccgg tggcaaccga cgctccggtt gatttcgccc cggcgctgtt
      841 caaggataac ttcatcccga ccccggttgt gatggtgcgg cgctctgcgt tggagcacgt
      901 cggcttgttt caacctgagc tgcggcaagc gcaggactat cacctctgga cacgcctcgc
      961 ctgccacggg cgcggcgtat acctcagcgc tcctctggcg gtgattacca ccgatgaccc
     1021 ggacagtgtt tcccgcgacg ccgcgcgcct gctgcgctgg agcctgcgcg cccgccaact
     1081 tctgcgtcgc gaactgcgcg ttagaccgga ttgccgcccc gcctggcggc gcggaatggc
     1141 cgcgtgcctg gcccaactgc gcgatgaatg ctaccgtcgc agggaattcg ccgacgcgac
     1201 ccgctttggc gccgcttcgc tgatgtaccg gccgtggccg cggcccaagt gggagttcgg
     1261 ccggctgctc gcctccgcag cccgagcggc cgtggaaccg gtcagaaaag ccagacattc
     1321 atcccaggtg cagtgctcgg ccacgtaatc ccggccgcgc tcccccatga cggcgcacgc
     1381 ctctggatcc cgcgcgagcg cgttcagctc ttcgcccagc cgccccgcgt cgcgcacgta
     1441 gcaccgcccc gaccccacat gcctcactgg cttcgccaag cccgaataca cgcgcggcgg
     1501 gtcgtgctcc ggcacgagga ccggccggcc tgccgccatt ccttccaaaa gcgtatatcc
     1561 caacgactcg tagaagctcg tcgaaaccac gcaatccgcc gcagcgtacc aggacgtgac
     1621 cgagcgttgc acgccgggaa gatgaacgcg gttggccaaa cccagatcgc gcgccaagcg
     1681 agccagatta tcgcgctcag ggccgtcgcc aatgatggcc agatgcacgt gtcgcggcgt
     1741 gccgactagc gcgcggatcg ccagctcaaa gcctttgtta cgatcgcaag tgccgctcgc
     1801 aacaaccaga aactcgtcac ttgtcaaacc gttcgcctgc cgcgttcggg cacgttcctc
     1861 ttgcgccact tgtacgcggc gcactccgta actaacccgc tttagtcgcc cggccagccg
     1921 gggtgaatag gcgcggagtt cctcaaaagc cgcgtcggtt ggtacgaacg tggcgtctgc
     1981 gagctgaaac gcccggcgtt ccacccggca aattgcccta aaatgcacac gtgcccacag
     2041 gttcggcgcc cgccgctgag accagccaaa aggcaggcaa ttcgccagca agcacgggta
     2101 aagaaagtac accggcactt tcggccaggc gcgcttcgct gcaatgatcc agaaaggact
     2161 gactgcgaca aaggccattt tcggcggcgg cagatcggcc aagacgcttg ccagcgcccc
     2221 aggcacaagc cattgtttga atcgccacag ccgtcgcggc actcgcagcg gcacgttcaa
     2281 agcctcggcc tgcggcttgg gcggcccctc gggttttccc gactcgctat tcacacccag
     2341 cacgagcgtg ctgtgtccgg cctgttgcaa cgcttcagcc aacgagcgcg cagaagtgag
     2401 accgccaccc caactcgaaa catcgcagta cacaccgaaa taaaagctgc gcggctgtat
     2461 gcgagtcact gagcgcattg acttccgtta gtgttatcgg ccgcctgcgc tcaggggtga
     2521 agccggtttc agcccgcgat gtcctgcacg accacatgga tgtcgtgctg agccaattcg
     2581 gccatgtact gctcggtcgg cagacagagt tccagcgggc gcgctccggg cgcgagcagc
     2641 cgccgggcct gcatgtaggc cacgaggctc gcgggaaaag cggtcgtgcg ttccatggcg
     2701 gtgaagccgg tctgttcgtc gtgccggtca aacaggtcgt attgcagcgt ccgtggcgcg
     2761 ccgcgatgcg tgccgctgac cgtgcagcgc agcacgacca ggtcgcgaac ttcaggaaag
     2821 cgcagccggt cttccatgag ctgccgcagc acgggcttcg gcgcgatcac ttggccatca
     2881 gaaagagtca cgcgctcctc gaacaagcct aactcaaaca tggcccgcac gaccgcaaaa
     2941 tgcccgggat agcgcacggt cttgtaatcg taagtgcgta gccggccggc gaagctggcg
     3001 gcgcacgtgc tcgtcccgcc cgacgtcacc gcggcttcgc actttccgag cggcggcgca
     3061 aactcgatct cctcaagttc ggtcagcgcc ggcaccgcag cgcgccggcc gtcgcgcaga
     3121 aattcgccaa agccactgta ctcgttgacc aggccatcga agttgaacac cagcttgtag
     3181 cccagcgggc cgacgggctg ctgcggcagg ccgccgcagc gtacgtgtac gtcgcgcggc
     3241 gcgtccatct gactcacgcc gtgcgccgca agtatgttcg ccaacccggg cgccaacccg
     3301 cagtccggga ccacgctcac gccgcgggcc tcagcggccg catgctgtgc aagctgttgc
     3361 cgcacaacgg tcgtgttacc gccgagatcg cagaacgacg cccccgccgc aatcgccgcc
     3421 tcagtcaatt cggcattgaa ccggtagggc gcagccgaga ttaccacgtc agcgccggcc
     3481 agcgcgtgag ccacttccac aggcctgctc acgtcacaaa ttactgcctc gaattgcgtg
     3541 gccatcgccg gaagaagcgg ccgcagacgt gccagcgcac tgtcaacttg agcaggactg
     3601 acgtcggcca gccggacgcg cccggcttcg cagttttttg ccaaatcgta agccagggcc
     3661 acaccctgac gcccggcgcc caagactacg taagaaaagg ccatgctgag ctcccgtcgg
     3721 gcaccctgcc gctacgttct actattccaa atcggatcgc tcacgccgca tttctgattg
     3781 tggaaccggg ccatgacaaa cagcaaatcc gaaagtcgat tcaagtacgg aacattgtcc
     3841 gctccgaccg gttcttgtgc cgcgagcgca acaacgatcc gctccgcacg ccggcagacc
     3901 gtccgcgcca cgtgcagaaa cgcggccacc ggcgtgccgc ccggcaacac gaaattcttc
     3961 aacgccggca ggtcctggtt cagagaacta aggcaatttt caagcgcggt tacgtgctgc
     4021 gcactgagcc gcgggctggg gtgcttcggc ttatccgcct cgggaatgct caaatccgct
     4081 cccaggtgga acagctcatt ttgaatccgc cgcaacggtt cgaccagctc cggcaccggc
     4141 ccggccgcca gcaccgttcc gatatgcgcg ttgagttcat caatcgtccc gtaagcttcg
     4201 atgcgcagcg cgtttttcgg gacgcgccgg ccgctgccca ggccggtcgt gccatcgtct
     4261 ccggtccgag tatatacgcg cgtgatgcga gccatgcgtg ctctccaggc gggcggcggc
     4321 ggcgcaaggg ccgcgagtcg atccggcccg gcctccgagc ttacaggtta ccgtttttcg
     4381 cgccgcacgg gaacatttcg taaactgagt attggggggt cgagatcgga ccagcggccg
     4441 tatttgagcc ggcagggtgt gattcgccgc accggctgct cagtggcgga ggacttcaaa
     4501 tggacaactg gaagcagacg tttcttgaca agctcactca ggcccaatcc cagtggatca
     4561 gccgctttga tgaagccctc aacacccatt ttgtgcccgt tttcgaggaa tatcggcagt
     4621 tcctggccga caacgggttt cggctgtccg tgccgatgcg cgagcccggg cggcgctcgt
     4681 tcaaatttga gctgtcagag aacgcgtatc tgctgatgat cctgcgtgcc atgggtgtgg
     4741 gcgaactgga gctgcgctgc gaaagtttcg tgccgggctg cgacccggct cattcgcgca
     4801 gcgtcgggct ggtttcgcag gtgacgcagg agtgggtcac cgagcgcgtg cgcggcgcac
     4861 tcgagacgtt cgttgacctg ctcagcaagg cggtgccgcc tgaggtttcc gaaccgctcg
     4921 aggagctggt gagcgcgtag cagaccacgc ggccgcaatc gagatgggtt tggcgcgccg
     4981 gtttcgccgt cagagttgat attgcttctg ctcgcccgcc gcttcgccca gtgtcgcgcg
     5041 gaagaagtcc accactttga cgtcttcagc cgccggcagt tgcccgtgct cggccccggc
     5101 gaccaggctc aatgtgacct gcacgccggc cgcggtgagc cggccgtaca gttcttggct
     5161 ctgactgggc ggacagcgcg tgtcggccgt cccgtgtgcg atgaagaacg gcggatcgtc
     5221 gggcgtgacg gaatagaccg gactcgccag ccgcgccaac tccacccatt gcgcccactc
     5281 gggatcgtca gcatgcgcca caatatcgcc cagacaacgc cccagaagcg gcggctcgcc
     5341 tgagccgcag tcatccacgt gcgcggcaaa cgtgaaagcg ttggtcgggc catagtagtc
     5401 cgccaccgcc tgcacgcggc tggagcaatc cagattgccg cccacgtcgc cttccaactc
     5461 ggccacatcg ccgctggttc caaccagtgc ggccaaatgc ccgcccgcgg aacggcccca
     5521 cacgccaatc cggtccgcat ccagcgcgta ctcgcccgca tgggcccgca gccagcggat
     5581 cgcgcctttg cagtcattaa tctgcgccgg gaacgcagcc tcgccgctga gccggtagcc
     5641 aatgctcgcc actgcgaagc ccgcctcggt cagcaattcc gcaggataag ccgggtattt
     5701 ctcgccgccg ctccagcgcc cgccgtgaat ccagacgatg acggggcgcg gcccgccggc
     5761 gtcatgcggc aaatacaaat cgagtttctg acgtggatgg tcaccatacg ccagatcgcg
     5821 gaacgttggc tggatttcga acgggttagc ttggaatgcc gagcaacctg cgagcaaagt
     5881 tgccgccatc agcaaagcga actgaattgc tcgaacggcg gacactcgaa cgccggacat
     5941 acgaatctca gccccaacac acctcatgcg acgagtatag tgcaacatgc cagagccggc
     6001 acgcgcctca catctgccgc tcgcgccgca gcgcgcgcac catcagcacc agcagcgccg
     6061 ccaaaatcgc accgagatat ttgagctcat cctcccaggc ccactcggag ccgacgaacc
     6121 aactcggcac gatcaaagcg ccccatgcca ccagtatagc ggcgcgtgtc ggcctcacgg
     6181 cgactcctcc gcccggatgc ggtatatgac ggcgctgcca tcgtccgcgg taaagcaacg
     6241 ctccagcgcc gggcaggcgt cccaacgttc gatggtcgcc gcgccgcctt cggtcgtctg
     6301 gaaaagcagg taacgcgcgc catacttgcg ggcggtttcg gccgccgctg caggttcttt
     6361 ccccgacaca agcgcgtgca acacatcgtg ccgctgccgc acatccggcg cggggttcat
     6421 ctgcccgatc ggaacggcga tgcacttgcg tccggtcagg cccgacacga ctttgtagcc
     6481 cagttcagga tcgcaggcga tcgtgtcttc gagcgcggtg tgcgtttgaa tccaggcccg
     6541 cacttgccgc gtttgctctt ggagctgagc gacactgaca aaatagctgt cgtccggcgc
     6601 taggtgccac attgccggcc ccaccgccaa cacccccaac acgatcgccg ccgccgggac
     6661 cagacgcgga cgcctggcgg cctgcgccag ttgtcgcgcc agccacatga tcgccacggc
     6721 cgcgcataac ccttccgcaa tttgcatgtg ccactgcgcc tcgtgcggca gcaaatacgg
     6781 tgcgttcagg cccagataac gcgccaggta agccggaatt tgcagcagca acgcggcaag
     6841 taaataggcc accgcccaaa atgtctgggg cgcgtgtcgc aagatgaccc acacccccac
     6901 gagaaaggcg gggacgacca acggggtata ggcatgcgca taaaacggcg ggcgcgtaac
     6961 ttccgccgcc atgtattgca gcagtcgcgg attttgggcg tggaccgcga ccatgtgcac
     7021 aatgagcggc agcgttagca ctagcgcagt gcccgccacg accagcatgc gccccagcag
     7081 ccgctggcgg cacttgcgcg ggc
//
LOCUS       DAOSYS010000187         7093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8970_length_7093_cov_5.231742, whole genome shotgun sequence.
ACCESSION   DAOSYS010000187 DAOSYS010000000
VERSION     DAOSYS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8970_length_7093_cov_5.231742"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="PLU99_11420"
     CDS             complement(<1..2237)
                     /locus_tag="PLU99_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO17704.1"
                     /translation="MPQAFAGAVRVDAAGPSCVVRFTASFDSDSLVFERHRDFDIVRL
                     PEANFLNEPGLPKLPVAALRIALPAGMAVTGAKATGVAAVDLPGSYAIAPAQPVQRIS
                     DPPGAFVPPDANVYSSTEPYPVNLIRSVRQNDLAGQSMATVEVCPVQYIPATGQLLIN
                     TAIEITIEGTAGYVCGDYLSPAISAQEQAARALEVGEMVLNPEDVQLAPPLLLDGGSR
                     AVTPEYYPYVIITVEEWVSAFQPLADWKTKKGVPARIVTTAWIYNNGEYSGSDQAKIR
                     SFVQDAYTNWGTRYFLLGGDTNVIPTGMRHVIDDDIPNDTYYADFDNDWICEVHVGRA
                     PARTSSQANVFVNKTLVYQKTPPADFGNWAALLGFDLHHEGSAEGEDCKQLIRTSYLD
                     GWTCHREYDSQPGGHLSDTINLLNQGHNLTNHIDHSSTYSMGAGSINHGTYLSNSIMA
                     GLTNGARQGILYSIGCWACDFDSETCIAEAFVRNPGGGGVAFVGNSRFGWYVPGSPDA
                     ASGHFDRLFFRSLFDQGHYILGNCFSDHKNDAYDGGSYTQYIFTELTLLGDPELPLWT
                     AEPAPLSVVHPASIPLDAFTDFSVHVTSAGSPVANATVCLWKPGDVYELAQTDSDGNA
                     NFWFFASDLGNLHVTVTKPNYVPYEGQSPVIPSTGPYALFVLVHGAGNVLVTPDGETY
                     APGTSVEVDAQPAPGWCFDHWEYSLSGTTTPVTVLMDANKIIVAVFAPDCNANNRDDA
                     VDIATG"
     gene            2884..3912
                     /locus_tag="PLU99_11425"
     CDS             2884..3912
                     /locus_tag="PLU99_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006432937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO17705.1"
                     /translation="MNGDRRKIVVVTGASAGVGRATVREFAMQGAWLGLLARGRDGLE
                     AARRDVEQLGGRALVLPTDTSNYEQVQAAADQVEREFGPIDCWVNNAMVSVFSPVSEM
                     LPEEYRRVTEVTYLGYVHGTLAALKHMRPRNRGIILQVGSALAYRGIPLQSAYCAAKH
                     AVQGFCDSLQCELLHDNSGVRLTMIQLPAINTPQFGWVKSRMPRKARPVPPIYQPEVA
                     ARVIVWAAGQNRREVYVGIPTVKAIVANKVSPRLLDHYLARNGYASQQRGEADDPNRP
                     HNLWEPLPGDHGAHGEFDAEARDFSPQVWLTQHRGRLLIAGAALVAAFIGNRWVQAAR
                     RDARAVES"
     gene            complement(4004..4101)
                     /gene="ffs"
                     /locus_tag="PLU99_11430"
     ncRNA           complement(4004..4101)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU99_11430"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4220..6157)
                     /locus_tag="PLU99_11435"
     CDS             complement(4220..6157)
                     /locus_tag="PLU99_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO17706.1"
                     /translation="MYWLLGAAALGILAAGVEVFFHVQRTQASGPAGLNVLLITVDTT
                     RADFLGCYGRSNGRTPNMDGLAREGVLFQRCISASPMTGPSHASIFTAAYPFVHGARR
                     NGTHPLVDANVTLTEILKKAGYRTQATIANFILNRQFGFSQGFDVYHDVSAAAAADPQ
                     SSERKGDEICNDALTLLRTPGPEPFFLWVHFFDPHAPYETKRPAPYTVAEAYEDEIAF
                     MDEQIGRLLGELRSLGIADRTLVVLVGDHGEGLGEHGESQHGHFLYETTLRVPLLMRS
                     PGKIPAGRSVPELVRTIDIAPTVLALLNLPPLENVQGVSLLPLVQSNGTSPELRAYSE
                     TLEPHAQFGLSRLRSLTTPEWKYILAPRCELYRRQTDPAEAHSLTQQEPAVAVALRQE
                     LKDVIAHSPPAVSAGAAPHQLTEAERARLESLGYAGTGVRGEDPNQSELDRFEPEGGN
                     PADYAAQMELADEARDALGTGDFARAQQLLETALQVLPDAPHIHANLGLALSAQGRSP
                     EALQHFERALALAPDDSNLRSTYGMLLVQLGRFQEAVDQLRIVLRPDRKKPMVLQAIA
                     FALTQLGEFDEAEQHLQWALEIDPRSPRVLHMLGVVRIRQNRLAEAEPYLSRALEIDP
                     NFRQCQEDLHRLRTLLEPPNP"
     gene            complement(6327..>7093)
                     /locus_tag="PLU99_11440"
     CDS             complement(6327..>7093)
                     /locus_tag="PLU99_11440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOO17707.1"
                     /translation="CRSHTHIFYFTAHPTRFTFNDHALRFPSARHTEYQDLRASPKGR
                     LPDDVWTEFPRVCGTFKERAGFHGCQMPEALLMRIVMAASNPGEIVLDPFVGSGTTAV
                     AAKRLGRKYIGIDLSEEYARHTEERLANVRDESSAPAQPGNWPNLHAQMLAQIYAETK
                     VSLVNLLPNKVALKVITRSLKARTGAAYTVTEVARQLEHMRGALPKLPNDMPFVPRNH
                     VTEEAKRYQRRIFRCKPRSKKTDAWAEDQQTLRTVI"
BASE COUNT         1297 a         2105 c         2367 g         1324 t
ORIGIN      
        1 ccggtggcga tgtctacagc atcgtcgcga ttgttggcgt tgcaatcagg agcaaacacg
       61 gcgacgatga tcttgttggc gtccattaac acggtcacgg gtgtagtggt cccgctgagc
      121 gagtactccc agtgatcgaa acaccagccg ggggcgggct gggcgtccac ctcgacgctg
      181 gtgcccgggg cgtatgtttc gccgtccggc gtgaccagca cgttgcccgc gccgtggacc
      241 aggacgaaca acgcatatgg gccggtgctc ggtatgaccg gagattggcc ctcatagggg
      301 acgtagttgg gcttggtgac ggtgacgtgc aggttcccca ggtcggaggc gaagaaccag
      361 aagttggcat tgccgtcgct gtccgtctgg gccaactcgt agacgtcacc cggtttccac
      421 aggcataccg tggcgttggc caccgggctg ccggcgctgg tcacgtgcac cgaaaaatcg
      481 gtgaaggcat cgagcggtat ggaagcggga tgcacgacgc tcagcggcgc cggctccgca
      541 gtccagagcg gcaactccgg atcgcccagg agcgttaatt cggtgaagat atattgggta
      601 taactgccgc catcataggc gtcgttcttg tgatccgaga aacagttgcc caggatgtag
      661 tggccttgat caaaaagcga gcggaagaag aggcgatcga aatgcccgga ggccgcatcc
      721 ggagagcccg gcacgtacca gccgaaccgc gagttcccga cgaaggccac accgccgccg
      781 cccgggttgc gcacgaacgc ttccgcgata caggtttcgc tgtcgaagtc gcaggcccag
      841 cagccgatcg aatacagaat tccctgccgc gcgccgttgg tcaaaccggc cataattgag
      901 ttgctcaggt atgtgccgtg attgatgctg ccggcgccca tagagtaggt gctggagtga
      961 tcgatgtggt tcgtcaggtt gtggccctgg ttcagtaagt tgatggtgtc cgagaggtgg
     1021 ccgccgggct ggctgtcgta ctcgcgatgg cacgtccagc cgtccaggta cgaagtgcgg
     1081 atcaactgtt tgcagtcttc gccctcagcg ctaccctcat gatgcagatc gaagcccaag
     1141 agcgcggccc agttaccgaa gtccgccggc ggggtcttct ggtagaccag cgtcttatta
     1201 acgaagacat tggcctgcga ggaggtgcga gcgggggcgc gccccacgtg aacctcgcaa
     1261 atccagtcgt tatcgaaatc ggcgtaatac gtgtcgttgg gaatgtcgtc atcaataacg
     1321 tgacgcatac ctgtgggaat cacgttcgtg tccccgccga gaaggaaata gcgggtgccc
     1381 cagttggtat acgcatcctg cacgaatgaa cggatcttgg cttgatcact accgctgtat
     1441 tcgccgttgt tatagatcca agccgtagtc acaattctgg ccggaacacc tttcttggtc
     1501 ttccagtccg ccaggggctg aaaagcgctc acccattcct caacggtgat gattacgtac
     1561 gggtaatatt ccggggtcac cgcgcgcgag ccgccgtcca gcagcagcgg cggcgcgagc
     1621 tgtacgtctt cggggttgag caccatttcg ccgacttcca aggcgcgggc ggcctgctcc
     1681 tgtgcagaga tcgccgggga aagatagtcg ccgcaaacgt accctgccgt gccttcgatt
     1741 gtgatttcaa tcgcagtgtt gatcagaagc tggcccgtgg ccggtatgta ctggactggg
     1801 caaacttcta ctgtcgccat gctctggcct gccaggtcat tttggcgcac cgacctaatt
     1861 aaattgaccg ggtacggctc agtggatgag taaacgttgg cgtccggcgg cacgaatgcc
     1921 cccggtggat cggagatgcg ctgcacgggc tgagccggcg ctattgcata gctgcccggc
     1981 aggtcgaccg ccgccacccc ggttgctttg gcgccggtta cggccatgcc cgccggcaag
     2041 gcgatgcgca gcgccgctac cggcagcttg ggcaggccgg gctcgttcag aaaattggcc
     2101 tccggcagac gaacgatatc aaaatcgcgg tgccgttcga agaccaacga atctgaatcg
     2161 aagctggcgg tgaaacgaac cacacaggac ggcccggccg cgtccacgcg gacggctccg
     2221 gcaaacgctt gtggcatctg agccgccaag ctcgccacga tcggcagcgc taacagacga
     2281 agaagcgggc aatacgcgac gcgagattgg cgccgactcg agagcatgcc tacaatcgac
     2341 aacacggaag agcctccttc ggcggtgggc atttcgacac tatcggcttt tcgagcatgc
     2401 gaccccgcgc actaaccaac tcctgggctc gcataggtag ccgaaggcca aataaagcgc
     2461 ctgcgctcgc gagcgcagcg caaccaagtt ctatcgaacg cattataatc gtcttgcccg
     2521 gcgatggtaa cgctttttgt cgctcaagtc cgagtgtaca cttcggattt gcgataagtt
     2581 cagttgaggg aaggagttaa ggtgcgtcgg cccgggcggt tggggttccc tttttggtct
     2641 cataactgta gccaagatat cggtgttcgc acgtgtcgag acacctcctt ctggacatgc
     2701 tgacaaacac ccaattccga gctgcgtcga cgaatgcgtt cgaggtgccg gacacgccga
     2761 gtcgccggtc gccaagaagc tcgcccggcc ttgctctccc tcgcttgggt ggataacgcg
     2821 ctaacctaga gcaggcgcat ccaactagtc ttgcccgcgg ctggccggcc ggtgggagct
     2881 gttatgaacg gtgaccgcag aaagattgtg gtggtaacgg gggcctcggc aggcgtgggg
     2941 cgggcgaccg tgcgcgagtt tgcaatgcaa ggtgcgtggc tcgggttgct ggcgcgcggc
     3001 cgcgatgggc tggaggcggc acggcgcgac gtcgagcagc tcggcggacg ggcactagtg
     3061 ttgcccacgg acacctcgaa ttacgagcag gtccaagccg cggctgacca ggtagaaagg
     3121 gaattcggcc cgatcgactg ctgggtcaat aacgcgatgg tctccgtgtt ctctccggtg
     3181 agcgaaatgc tgcccgaaga gtaccggcgc gtcaccgaag tcacctacct gggctacgta
     3241 cacggcacac tggctgcgct caagcatatg cgcccgcgta accgcggcat catcctgcaa
     3301 gtcgggtcgg cgctggcgta ccgcggcatt ccattgcagt cggcctattg cgcggccaag
     3361 cacgccgttc aaggtttttg cgactccctg caatgcgagc tgctgcacga caacagcggc
     3421 gtgcggctga cgatgattca gcttccagcc atcaacacgc cgcagttcgg ctgggtcaag
     3481 agccgcatgc cgcgcaaggc caggccggtg ccgcccattt atcagccgga agtggcggcg
     3541 cgcgtcattg tctgggcggc cggccagaac cggcgcgagg tgtacgtcgg aattcccacg
     3601 gtaaaggcga tcgtagccaa caaggtttcc ccgcggcttt tggatcatta cctggcccgc
     3661 aacggctacg cgagtcagca gcgcggcgag gcagacgatc ccaaccggcc gcacaatctc
     3721 tgggagccgc tgcccggaga tcacggtgcg cacggcgaat ttgacgcgga ggcacgggat
     3781 ttcagtccac aggtgtggct gacgcagcac cgtggccggc tgctgatcgc gggcgcagcg
     3841 cttgtcgcgg cgtttatcgg taaccgctgg gtgcaagctg cccggcgaga cgcgcgagcg
     3901 gttgagtcgt gactgtatgg gtttattacc ccgttcgctt caagcagtgt cggcagggtg
     3961 ccttgcgcgt gcggccggcg caagaaaaaa cgtgcccgag gctttaggcc aggtttacga
     4021 tgcacgtgga ccgagccgga tgggctgctg ccttccgacc ctgacccgtt gtccgaccgg
     4081 tgcactgcac cggcccaacc gtccacctcg gacacgacca atgatgatac caccggggcg
     4141 gccaggctgc cagccgcggg ccgaacgcgc tcggcgcggg tgtgacggcg cgtgatgatg
     4201 aagcggaaga agggcgccgt tacggattcg gaggttccag gagcgttctg aggcggtgca
     4261 ggtcctcctg gcattggcgg aagttcgggt caatctccaa agcgcgggat aggtacggct
     4321 ccgcttcggc caggcggttt tgacggatgc gcaccacgcc cagcatatgc aggacacgcg
     4381 ggctgcgcgg gtcgatctcc agggcccact gcaggtgctg ctcggcttcg tcgaattccc
     4441 ctaactgcgt gagtgcgaac gcgatcgcct gcaggaccat cggttttttc cggtcggggc
     4501 ggaggacgat ccgcaactgg tcgaccgcct cctggaagcg gcctagttgc accagcagca
     4561 tgccgtaggt gctgcgcagg ttgctgtcgt ccggcgccag ggccaaagcg cgttcgaaat
     4621 gctgcaatgc ctccggcgac cgcccctgcg ccgacagggc caagcctaaa ttggcgtgta
     4681 tgtgcggcgc gtccggcaga acctggagcg ccgtttcgag caattgctgg gcacgggcga
     4741 agtcacccgt gccaagagcg tcgcgggcct cgtcggccaa ctccatttgc gctgcgtaat
     4801 cagcggggtt cccgccttcc ggctcgaaac ggtcgagttc ggattgatta ggatcctccc
     4861 cgcgaactcc cgttccggcg tagccgagcg attccagccg ggcccgctcc gcctcagtca
     4921 actgatgggg cgcagcaccg gcgctgacgg ctggaggcga gtgggcgatg acgtccttca
     4981 actcctggcg cagagccacg gccacggcgg gctcctgttg cgtcaacgaa tgcgcctcgg
     5041 ccggatcggt ttgccggcga tataactcgc agcgcggggc gagaatgtat ttccattccg
     5101 gcgtcgtcag gctccgcagg cgagataagc cgaactgggc gtgcggctcg agcgtctcgc
     5161 tgtaggcgcg cagctccgga ctcgtgccgt tgctctgcac taacggcagc aggctgacgc
     5221 cctggacgtt ctccaacgga ggcaaattca acaaggccaa aacggtgggt gcgatgtcga
     5281 ttgttcgcac aagttcgggg actgatcggc cggcgggaat cttcccgggc gagcgcatca
     5341 acagcggcac gcgcagcgtt gtttcgtaca gaaagtggcc gtgttgggat tcgccgtgct
     5401 caccgagacc ctcaccatga tcgccgacga gcacgaccag cgtgcggtcc gcgataccca
     5461 ggctgcgcag ctcgcccaac aagcggccga tctgctcgtc catgaaggcg atctcgtcct
     5521 cgtaggcttc agcgacggtg tagggcgcgg ggcgtttggt ttcgtacggg gcgtgcgggt
     5581 cgaagaagtg cacccacagg aagaacggtt ccgggcccgg cgtgcgcagc agagtcaggg
     5641 cgtcgttgca aatctcatcg ccttttcgct cgctgctttg cgggtcggca gccgcggcgg
     5701 cgctgacgtc gtggtacacg tcgaaccctt gcgaaaagcc gaactggcgg ttgaggatga
     5761 agttcgcgat cgtggcttgc gtgcggtagc ccgctttctt caggatttcg gtcaaagtaa
     5821 cgttggcatc aaccagcggg tgcgtgccgt tgcgacgcgc gccgtgcacg aagggataag
     5881 cggccgtgaa gatgctcgcg tgcgaagggc cggtcatggg cgaggcgctg atgcagcgct
     5941 gaaacagaac gccttcgcgt gccaggccgt ccatgttcgg cgtgcggccg ttcgatcgcc
     6001 cgtagcagcc gaggaaatcg gcacgcgtgg tgtctacggt gatcagcagt acgttcaggc
     6061 cggccggacc agaagcctgg gtgcgctgta cgtggaaaaa aacttccaca cccgcggcaa
     6121 gtatgcccag ggcggccgcg ccgagtagcc aatacatcca gggacgccga ggggaggcgg
     6181 gcggtgacac ggctggcttt ttggaggtga ttcggctgcg atcgcgcggc tgcatggcgg
     6241 ctccgtcgcc gcatcggtct cgctcaccga gaacggctgg ccgcagtata agttgcgcgg
     6301 cgagcgtgct tcaagccgcg gcgcggtcag atcacggtac gcaacgtttg ctgatcctca
     6361 gcccaggcgt cggtcttctt gctccgcggt ttacagcgga agatgcggcg ctggtagcgt
     6421 ttggcttcct ccgtcacgtg attgcgcggt acgaaaggca tgtcgttggg cagcttcggc
     6481 agagcgcccc tcatgtgttc gagctggcgg gcgacctcag taacggtata cgcggcgccg
     6541 gtacgggcct tgagtgaacg ggtgatgact ttcagggcca ccttgttcgg cagcagattg
     6601 accagcgaga ctttcgtctc tgcataaatc tgggccagca tttgagcgtg caggttcggc
     6661 cagttgcccg gctgcgccgg cgccgaactc tcgtcacgga cgttcgccag gcgctcctcg
     6721 gtgtgccgcg cgtattcctc tgacagatcg atgccgatgt acttgcggcc gaggcgtttg
     6781 gcggcgaccg ccgtagtgcc gctgccgacg aaggggtcga gcacgatttc gcccggattg
     6841 ctggcggcca tcacaatgcg catgagcagg gcttcgggca tctggcagcc gtggaagccg
     6901 gcgcgctcct taaaggtgcc acagacgcgc gggaactcgg tccagacgtc gtcgggcagg
     6961 cggcccttgg ggctggcgcg caggtcctgg tactccgtgt gccgcgcgct ggggaagcgc
     7021 agggcgtgat cgttaaaggt gaaccgcgtg gggtgggcgg tgaaatagaa aatgtgcgtg
     7081 tgcgaccggc aga
//
LOCUS       DAOSYS010000188         7091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_8974_length_7091_cov_5.295338, whole genome shotgun sequence.
ACCESSION   DAOSYS010000188 DAOSYS010000000
VERSION     DAOSYS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8974_length_7091_cov_5.295338"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PLU99_11445"
     CDS             complement(<1..1143)
                     /locus_tag="PLU99_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17708.1"
                     /translation="MLNAQPPQTTTDALRPDIGALSRRIRRLGTELHSAVRAVTQQVA
                     TTSRKPHELARSLRIHRTLASRLLNAVRTDDPLAAISRMPRSEGLRMFLTAAKSSASR
                     DAIEQAETALRNFEHLVDGELGGWDGFDAVITEWLPDAREKFELANKQAAFKGMANLL
                     GVRADVQLVTSIYYPDADGQSCDIAVIEGMAGLRRLRPSARIPFAMTAPIPGAPRPLA
                     YVLEGLTHAEPGPDYPLLDEFCSKPIPRFHAVRAGKITNYFLAGNEVGENSAVDVYAA
                     SVIRHARPIYADAAAPGWRPSAAAGPNVPAKVLLLNFLVHEDIWPESDVELRVYDTHV
                     NGLADANDPARDIDRLELQESVQVLGKGAARFRATEVGRHVEMVQYV"
     gene            1365..2684
                     /locus_tag="PLU99_11450"
     CDS             1365..2684
                     /locus_tag="PLU99_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17709.1"
                     /translation="MKEKWALAALAVMLSAGAGQGMADDLIVYVFDQDLKAVLRIQDM
                     NHDWDTRDPGEVVLAYDQYPAGTGVGNSQGMVALGPDDLLVTDNYAPPNIVRLRDLNG
                     DHDFFDAGEATVWFGGGLPGGRMLEMPVALIAGPDGAFYLFESSFGGSANPDAIYRLE
                     DLNGDGDVNDADEVELWVELSPGGISAVSANDLEFDNAGYAYFSDTPSGETLRVKRID
                     PVTREVTEYLTDDMLLSLTGYWIAWLYGGPLAYNYFTDEIVLFGYYGIATDVLLAIKD
                     RDGNGIIDKPNEVRELWHELSGTHAPEQDHGREYFFLPDGSLLVLDMLFDRVKRLSDN
                     NGDGDYNDSGETVVLYDAAQAALVGQPAATRLLSVTAMVVTTSGDVNCDSAVNVFDID
                     SFVLALTDMGKYRAAHPGCDPGSADINGDGAVNVFDIDPFVSLLVGG"
     gene            2971..3384
                     /locus_tag="PLU99_11455"
     CDS             2971..3384
                     /locus_tag="PLU99_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17710.1"
                     /translation="MMLQPTRDRLRRAYLALLWLACLCRYDLTGNLSGVCPECGRVRR
                     EHDDDIREPWRRQAARVALAYAVLAVVTAPLALRTFFCAFTLPSLSLLNIAIAIGIGH
                     LLLAPLLLPMAAYPSKRIARRMAYARAQPASNPTA"
     gene            3570..4730
                     /gene="sucC"
                     /locus_tag="PLU99_11460"
     CDS             3570..4730
                     /gene="sucC"
                     /locus_tag="PLU99_11460"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOO17711.1"
                     /translation="MKVHEYQARALLREAGIPVPASEVITTAEQAAEAFRRLVAETAV
                     VKAQVHAGGRGKAGYVQLVRSAREAVCHARRMFAEPMVSKQTGPQGVRVRKILLAKAV
                     DIAKEYYLGVVVDRARRVPVMMASAEGGVEIEEVAARNPDAIKKAWFHPHLGFQQFQA
                     NALAAAIGFTGLRAKVVADIMMKLARLFLEKDCSLAEINPLVQTIDGEVMAIDAKFNF
                     DDNALLRHEDIAALFDASEENPAELRANEANLTYIALEGNIGCVVNGAGLAMATMDII
                     KLHGGEPANFLDVGGSVTPEAAVEAFRIILEDPKVKAILVNIFGGIAKCDVIADALIK
                     AAREVGFNVPVVVRLEGTNVDKAREMLKAANIPQLVPAADLTDAAKRVCAAV"
     gene            4828..6318
                     /gene="rfaE2"
                     /locus_tag="PLU99_11465"
     CDS             4828..6318
                     /gene="rfaE2"
                     /locus_tag="PLU99_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOO17712.1"
                     /translation="MSATSQISLIERFTSSRVLLVGDFMLDRYVFGDAERISPEAPVP
                     VLRVIERQDRVGGAGSVALNVVALGGKVFCCGLLGKDEFGDRVEKALREAGCDAGGLL
                     RVNDRPTITKTRLVGLAEHRHRQQMLRVDDEIIRPMTADDERQMLARVKEYLPRVDLV
                     CLEDYDKGVLSPTTCQAIVAAARHACKPVLIDPARSPAWEKYAGATLLTPNRVEVEIA
                     FGRRIGQEELEPAGNELIRKYRLDALLITLGRDGVMLLRRDRAAQRFPTVPRAVYDNT
                     GAGDAVLAMVAVALAAGATLEQAATLSNIAGGLEVSKFGCVPITRDEVLRELRDGVQR
                     GRGKIRELGELCEELSARRQRGETVVFTNGCFDILHPGHVELLEKAKALGSVLVVAVN
                     TDASVRKLNKGSDRPIRRQEDRTRLLAALECVDYVVVFDEPDPLHLITRISPDVLVKG
                     SDWADKKVVGREHVESHGGRVVLVDLVEGYSTTAELERIRAGLNSR"
     gene            complement(6396..>7091)
                     /locus_tag="PLU99_11470"
     CDS             complement(6396..>7091)
                     /locus_tag="PLU99_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17713.1"
                     /translation="SLIGPNGSGKSTLLLAMLGCLRPQAGLLTLDGTPMHALPPRRRG
                     RFIAYVPQTIERLPAFTIYELVAGGRYPHVSPWRSLSLADREIVDQALETCGLTDLAD
                     RPVTAVSGGERQKALIAAAIAQDPQLLLLDEPTTALDPAVQVELARLLRDWHARGRAL
                     LVVSHELQLPAALGGRVLALRGGRIVADGPAGEVLRPAQLTSIFGADFVSGRTACGRE
                     FVLPAWVSAAKLK"
BASE COUNT         1249 a         2163 c         2440 g         1239 t
ORIGIN      
        1 gacgtactgg accatttcga cgtgccgacc cacctcggtg gcgcggaaac gcgcggcgcc
       61 cttgcccagg acttgcaccg attcctgcaa ttcgagccga tcgatatcgc gcgccgggtc
      121 gttggcgtcc gccaagccgt tgacgtgcgt gtcatagacg cgcagttcca cgtcactctc
      181 aggccagatg tcttcatgca ccaggaagtt gagcagcaga actttggccg ggacattcgg
      241 ccccgcggcg gcggaggggc gccagcccgg agcggccgcg tccgcgtaga tgggacgtgc
      301 gtgccggatg acgctggcgg cgtagacgtc caccgcggaa ttctctccca cctcgttccc
      361 ggcgaggaag taattcgtga tctttcccgc gcgcaccgcg tgaaaccgtg gaatgggttt
      421 ggaacagaac tcatcgagca gggggtagtc cgggccgggc tcggcgtgcg tgagcccttc
      481 gagtacatac gccagcggcc tgggcgcccc gggaataggc gccgtcatcg cgaagggaat
      541 gcgggcactc ggccgcagcc gccgcaaacc cgccatgccc tcgatcaccg cgatatcgca
      601 ggattgccca tcagcgtcag ggtagtaaat ggaggtaacg agctgaacgt cggcgcgcac
      661 gcccagcaag ttggccatgc ccttgaaggc ggcctgctta ttggccagct cgaatttctc
      721 gcgcgcatcg ggcagccatt cggtgatgac ggcgtcgaag ccgtcccagc cgcccaactc
      781 gccatcgacc aggtgttcga aattccgcag ggcagtctca gcctgctcga tcgcgtcgcg
      841 cgaagcggag gacttggcgg ccgtgaggaa cattcgcaag ccctcgctgc gcggcatgcg
      901 gctgattgcg gcgagggggt cgtcggttcg gacggcattc agcaggcggc tggccagcgt
      961 gcggtgaatc cggagcgatc gagcaagctc atgtggtttg cgggaggttg tcgccacctg
     1021 ctgagttacg gcccgaacgg cggaatgcag ttcggttccc aggcgtcgaa tgcggcgtga
     1081 cagggcaccg atatcgggac gtaaggcatc cgttgtggtc tggggcggtt gcgcgttcag
     1141 cattgccatc agtccattaa actgaaatga ggcgggcgtg tcaacagaac gagcccggat
     1201 atctctaaga atgctctgag agcattcttg acacagctcc agagttgtgg tatgttataa
     1261 gtcgactctg ctatgcgtaa gcggagttgg atgccgcgtg gcggccgcct ggaggcggaa
     1321 gtcgcatcct gccaacgagt tagttgacat ctggaggaat ggaaatgaag gagaagtggg
     1381 cgctggccgc gctggccgtc atgctcagcg caggggccgg ccaaggcatg gcagatgacc
     1441 tgatcgtcta cgtttttgac caggatctga aagcggtact gcggatccag gatatgaacc
     1501 atgactggga cacgcgcgac cctggcgagg tggtactcgc gtacgaccag tacccggcgg
     1561 ggacgggcgt cggcaatagc cagggcatgg tggcgctcgg cccggacgac ctgcttgtga
     1621 ccgataacta tgcgccaccc aacatagtta ggctgcggga cctcaatggc gaccatgatt
     1681 tctttgacgc tggcgaggct acggtgtggt tcggcggcgg cttgcccggg ggccggatgc
     1741 tggagatgcc ggtggcgctg attgccgggc cggacggcgc gttttacctg ttcgagtcga
     1801 gctttggcgg ttctgcgaat ccggacgcga tctacaggct tgaggacctc aatggggacg
     1861 gcgatgtgaa tgacgcggac gaggttgagc tttgggtgga gctttcgccg ggtggaattt
     1921 cggccgtcag cgcaaatgac ctggaattcg acaacgccgg ctacgcgtac ttcagcgata
     1981 cgccctcggg cgaaacgctg cgcgtcaagc gcatcgatcc ggtcacacgc gaggtgactg
     2041 agtacctgac ggacgacatg ctgttgagcc tgacgggata ctggatcgcg tggctctacg
     2101 gcgggccgtt ggcgtacaac tacttcacgg acgaaatcgt gctgttcggg tactacggca
     2161 tcgcgacgga cgtgcttctg gcgatcaagg atcgggatgg caacggcatc atcgataagc
     2221 caaacgaggt gcgcgagctg tggcacgagc tgtccggaac gcacgcgccg gagcaggatc
     2281 acggacgcga gtacttcttc ctgccggacg gctcattgct ggtgctcgac atgctgtttg
     2341 atcgcgtcaa gcggctcagc gacaacaacg gcgacgggga ctacaacgac tcgggcgaaa
     2401 ccgtcgtgct gtacgacgcg gcgcaggcgg cgctggtggg gcagccggcg gcgacccgtc
     2461 tgctgtcggt gactgcgatg gtggtgacca cgagcggtga cgtgaattgc gattcggcgg
     2521 tgaacgtgtt cgacattgac tcgtttgtgc tggccctgac ggatatgggc aagtaccgcg
     2581 cggcgcatcc gggttgcgat ccaggcagcg ccgacatcaa cggagacggc gccgtgaacg
     2641 tgtttgacat agatcccttt gtgtcgctgt tagtcggagg ctgaggcaga cagcagaagg
     2701 agcggtctgc aaaccggccg ggcgcttcgc cgcgcgtttt tcgcggttga cccgagtcgc
     2761 caggccggcc tcacagtgtc ctgcgtactg cgagccgtcg cagggcacgg ttaaaaccgg
     2821 ctgacgcggg ctatgaacct gcggcagccg gttttgtatt tgtttgcgtg ctctcagtcc
     2881 gccgccggcg cgcacatatc cgcagcgatt acggctatag tgcggcacgg gcagccactg
     2941 cgtgtcgctg cgaatgaggg tgcccgcttg atgatgttac aaccgactag agacaggctc
     3001 cgccgggcat atctggccct gctctggttg gcgtgcctgt gccgctatga ccttaccggg
     3061 aacctctcgg gcgtgtgccc ggagtgcggg cgagtgcgca gggagcacga cgacgacatc
     3121 cgcgaaccct ggcggcgaca ggccgcccgc gtcgcgctcg cgtacgccgt gcttgccgtc
     3181 gtcacggcgc ccctggcact gcgcacgttc ttttgcgcgt tcaccctgcc atcgctgagc
     3241 ttgctgaaca tcgcgattgc aatcggcatc gggcacctgt tgctggcgcc cctactgttg
     3301 ccgatggcag cgtatccctc caagcgcatc gccaggcgga tggcctatgc acgcgcccag
     3361 ccggcgtcca acccgaccgc ctaacgacga ggccttcgcc gacaatgtgg tggccgtttg
     3421 gcacgctgcg tcggccacgg ggggggccga cgcgaccttt gtgctttggc ctgcagcgcg
     3481 ccggtttcgc actggcccgg tctgccctga ctgggtagac tccccgcctg aaaaggtttc
     3541 cgcccgtaga ggcgacggag taaagttgca tgaaagttca cgaatatcag gcccgagcgc
     3601 tgctgcgcga ggcgggcatt cccgttcctg cgtccgaagt tatcaccacc gccgagcagg
     3661 ccgcggaggc cttccgccgt ctggtcgccg aaacggcggt ggttaaggcc caggtgcacg
     3721 ccggcggccg cggcaaggcc ggctacgtgc agctcgtgcg ctcggcgcgt gaggcggtct
     3781 gccacgcccg gcggatgttc gccgagccaa tggtttcaaa gcagaccggg ccgcagggcg
     3841 tccgcgtgcg caagattctg ctggccaagg cggtcgacat cgccaaggag tattacctgg
     3901 gcgtggtggt ggatcgcgcc cgccgcgtgc cggtcatgat ggccagcgcc gaaggcggcg
     3961 tggagatcga ggaagtcgcg gcccgcaacc cggacgccat caaaaaggcc tggttccacc
     4021 cgcaccttgg cttccagcag ttccaggcca acgccctggc ggcggcgatc ggcttcaccg
     4081 gcctgcgggc caaggtggtc gcggacatca tgatgaagct ggcccgcctt ttcctcgaaa
     4141 aggactgctc gttggccgaa attaacccgc tcgtccagac gatcgacggc gaggtcatgg
     4201 ccatcgacgc caagttcaac ttcgacgaca acgccctgct gcggcacgag gacatcgccg
     4261 ccctgttcga cgcttcggag gaaaacccgg ccgagctgcg ggcgaacgag gccaatctga
     4321 cttacatcgc cctggaaggt aacatcggct gcgtggtcaa cggagccggc ctggcgatgg
     4381 ccaccatgga catcatcaag ctccacggcg gcgagccggc caacttcctg gacgtgggcg
     4441 gcagcgtcac gcccgaagcg gcggtcgagg cgttccgcat catcctggag gacccgaagg
     4501 tcaaggcgat cctggtgaac atcttcggcg gcatcgccaa gtgcgacgtg atcgccgacg
     4561 cgctgatcaa ggccgcccgc gaagtgggct tcaacgtgcc ggtggtcgtg cggctggaag
     4621 gcacgaacgt ggataaggcc cgcgagatgc tcaaggcggc caacattccg cagcttgtgc
     4681 cggccgcgga tttgacggat gccgccaagc gcgtctgcgc cgcggtatag ccttgattgc
     4741 ccgcaactag agcagtgcgc gtgacgcggc cgcgttgcgg gtcgtctagc actggagaac
     4801 acggttgctc cggccggaag ccgatgaatg agcgctacga gtcagatcag cctgattgag
     4861 cggttcacgt cgtcgcgcgt gctactggtc ggcgacttta tgcttgaccg ctacgttttc
     4921 ggcgatgccg agcgcatcag cccggaagcg cccgtgcccg tgctgcgcgt gatcgagcgg
     4981 caggaccgcg tgggcggggc cggctcggtg gcgctcaatg tcgtcgcgct gggcggaaaa
     5041 gttttctgct gcgggctgct gggcaaggac gaattcggcg accgggtcga gaaggccctg
     5101 cgcgaggcgg gctgcgatgc cggcgggctg ctgcgggtca atgaccgccc cacgataacc
     5161 aagacgcggc tggtcggtct ggccgagcat cgccaccggc agcagatgct gcgcgtggac
     5221 gacgagatca tccgcccgat gacggcggac gatgaacggc agatgctggc ccgcgtcaaa
     5281 gagtacttgc cgcgcgtcga cctggtctgc ctggaagact acgacaaagg cgtgctgtcg
     5341 cccaccacct gccaggcgat cgtggccgcg gcccggcacg cctgcaagcc ggtgttgatc
     5401 gatccggccc gctctccggc ttgggagaaa tatgccggcg cgacgctcct gacgcccaat
     5461 cgcgtggagg tggaaatagc ctttgggcgt cgcatcggcc aggaggaact ggagccggcc
     5521 ggaaatgagc tgatacggaa ataccggctg gatgcgctgc tgataaccct cggccgggat
     5581 ggcgtcatgc tgctgcgccg cgaccgagcc gcgcagcgct tcccgaccgt gccgcgggcc
     5641 gtttatgaca acaccggggc gggcgatgcc gtgctggcca tggtggcggt cgccttggcg
     5701 gccggcgcga cgctcgagca ggctgccacg ctgtcaaaca tcgcgggcgg attggaagtc
     5761 agcaagttcg gctgcgtacc cataacccgc gatgaagtgc tgcgcgaact gcgcgacggc
     5821 gtccagcgcg gccgcggcaa aatccgcgag ctgggtgagc tatgtgagga gctgagcgcc
     5881 cggcgacagc gcggcgaaac ggtcgtcttc accaacggct gcttcgacat actgcaccca
     5941 ggacacgtcg agctgctcga aaaagccaag gctctcggca gcgtgctcgt cgtcgccgtc
     6001 aatactgacg cctcagtccg aaagctgaat aaaggcagcg accgccccat tcgccgccag
     6061 gaggatcgga cacgcctgtt ggctgccctc gaatgcgtgg actacgtcgt cgtcttcgat
     6121 gagcccgacc cgttgcacct gattacgcgc atcagtccgg acgttctggt gaagggctcc
     6181 gactgggccg acaagaaagt agtcgggcgc gaacacgttg aatcgcatgg cggacgcgtt
     6241 gtgttggtgg acctggtcga aggctacagc acaacggccg agctcgaacg cattcgggca
     6301 ggtttgaatt cgcggtaacg cagatttggg ggtctgcgat gaagcacttc aagcggcggc
     6361 agacacacac gtcccgcttg cgcgcctgct tgtcctcact tgagctttgc cgctgaaacc
     6421 caagccggca ggacgaactc ccgcccgcag gccgtacggc cggagacaaa gtccgcgcca
     6481 aagatggatg ttaactgtgc cgggcgcagc acttcgcccg ccgggccgtc cgccacgatt
     6541 cgtccgccgc gcagggcgag cacgcggcct cccagggctg ccggcagttg cagttcatgg
     6601 ctgacgacga gcagggcgcg gccgcgggcg tgccagtcgc ggagcaggcg cgccagttcc
     6661 acttgcacgg ccgggtccaa ggccgtcgtc ggttcgtcca gcagcaggag ttgcgggtcc
     6721 tgcgcaatcg ccgccgcgat aagggctttt tggcgttcgc cgccactgac tgcggtgacc
     6781 ggccggtcgg ctaggtctgt cagtccgcac gtctcgagag cctggtcgac gatctctcga
     6841 tcggcgaggc tgagggagcg ccagggcgac acgtgtgggt atcggccacc cgctaccagt
     6901 tcgtagatgg tgaaggccgg caggcgttca atggtttgcg gaacgtaggc gatgaagcgg
     6961 ccacggcgcc gcggcggcaa ggcgtgcatc ggcgtcccgt cgagcgttag taagccggcc
     7021 tgcggtctga ggcagccgag catggccagc agaagcgtgc tcttgccgga gccgttgggc
     7081 ccgatgagcg a
//
LOCUS       DAOSYS010000189         6953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9215_length_6953_cov_5.453175, whole genome shotgun sequence.
ACCESSION   DAOSYS010000189 DAOSYS010000000
VERSION     DAOSYS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9215_length_6953_cov_5.453175"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLU99_11475"
     CDS             <1..784
                     /locus_tag="PLU99_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17714.1"
                     /translation="IRAAASLAAVPPGELPDKLRQDLERATEELLAVSRARPDDSASH
                     HNLGNFHAARREYDLALSCYERAFQLLPDNIAPLVNASLVQNALGRNDQAEASLRRAL
                     QIEPGSAAANLNLGLLLGEMGRLAEAEAVLRRAFEADPQSATAAYNLAVICGQTNPEA
                     GIDWCRAAAQLQPDEPKYRHALAFYLRRGGDIEAAITTLRTLTDRDVAFADAYTLLGQ
                     IYEEARRMADAAELYRRAAANPRFPNGLRQQFAARAARLSPG"
     gene            complement(787..1269)
                     /locus_tag="PLU99_11480"
     CDS             complement(787..1269)
                     /locus_tag="PLU99_11480"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOO17715.1"
                     /translation="MSAESAIPETPDTSPVTAGLTNLDVGLFLIRLLPGIVFVFHGSQ
                     KLFGWFGGSGMTKFAEFLANQGVPSPAVAATIAALVEFLGGLALITGIGMRVMSLLLF
                     FNMAVASLLVHRHAFSAQHGGMEYPLTLAFIALGLAIAGPGSMTLPAAFRSVRSKDAG
                     "
     gene            complement(1581..2525)
                     /gene="cysK"
                     /locus_tag="PLU99_11485"
     CDS             complement(1581..2525)
                     /gene="cysK"
                     /locus_tag="PLU99_11485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOO17716.1"
                     /translation="MSRLFDDSARLIGNTPLVRINRAVRGGAAVYAKLEYFNPLKSVK
                     DRIGVSMIEEAIARGLVTPETVVIEPTSGNTGIGLAFVCAAKRLRLILTMPESMTLER
                     RAVLRALGAETVLTPAAEGMRGAIARAEQLLAEMPKAYMPQQFENPANVEIHRRTTAE
                     EIWNDTDGRADILVAGVGTGGTITGVGEVIKQRKPGFQCIAVEPATSPVITQARAGQP
                     LKPGPHKIQGIGAGFIPKILNLDIIDDVITVTDDEAFGWARRCAREEGILCGISSGAA
                     LCAADQVARRPENKGKLIVTVFPSAGERYLSTPLFQVD"
     gene            complement(2894..4039)
                     /locus_tag="PLU99_11490"
     CDS             complement(2894..4039)
                     /locus_tag="PLU99_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOO17717.1"
                     /translation="MKSAFTFSAFFLGLTAFSVAAAQQPAAPPAPQTAPASQPVSDVG
                     ARILLRPVAFDFGEVWQGAAAKGEFHIQNTGDTPLTTTVTSSCGCTVATKPKSPLQPG
                     ESDVFTITYDTRRIGPAGKKVMINSNDPTQPTITIDVNGTVKPIFAATPPDCNFANAE
                     ENTVATQTIRLENQYPEPLPLRIKPGQNIKQFEYELKEVEAGKLYELTVTTKPPLNQG
                     KNMTVLAMETGRPDLPDIELRLSANVMPRAQLSPYAVYVTPSRKEPWETVLNVQYRAD
                     KPLKILGVHCDLETIQCELMPHSPPAEGAILGYYQIKVKLPPFEEIPVDGAKIIIETD
                     DADPKFAKLEAPVERREGRRRSQSQPTTKRATGRTVVPSKAQPAPGK"
     gene            complement(4252..4324)
                     /locus_tag="PLU99_11495"
     tRNA            complement(4252..4324)
                     /locus_tag="PLU99_11495"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4289..4291),aa:Ala,seq:cgc)
     gene            complement(4528..4956)
                     /locus_tag="PLU99_11500"
     CDS             complement(4528..4956)
                     /locus_tag="PLU99_11500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOO17718.1"
                     /translation="MANAVLDQNCVFCKIIRGEIPCQRVYQDKHVLAFLDIQPLAPGH
                     TVVIPRHHAEFLDQLPEDWAVAIGRTLGRLGRAVVNASGADGFNVLQNNGRAAEQEVP
                     HVHFHLIPRHAGDGLGYRWKAKKAAPAELEKMAAAITSRL"
     gene            complement(5167..5883)
                     /gene="rplQ"
                     /locus_tag="PLU99_11505"
     CDS             complement(5167..5883)
                     /gene="rplQ"
                     /locus_tag="PLU99_11505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOO17719.1"
                     /translation="MRHNVAGRKLNRTTAHRRALRRNLVQSLIEHGEIRTTLPKAKEV
                     QRFAERLATLAIDGSLAARQRAIALLNDRAIIAKEQQEEYDKMSDAKRAKVLRARSGR
                     RYRASTTKPGVKFTADSVIHKLFAELGPRFKKRNEALGSSGGYTRVIKLAQRRLGDGS
                     DTALLQWVGEDDKKRPKSTEKTERKRRARAKYAAFAGKAAPRRAPRRSTRPSKEAEAK
                     PQAGVSAAQPEPGAEEKAEQ"
     gene            complement(5891..6931)
                     /locus_tag="PLU99_11510"
     CDS             complement(5891..6931)
                     /locus_tag="PLU99_11510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOO17720.1"
                     /translation="MRIRWRGLELPTRVVRDEALSTDTYGLFTVEPFERGFGTTVGNS
                     LRRILLSSLEGSAVTHARIAGAEHEFTSLPGVLEDVTDIILNMKALVVDLDSETPKTM
                     RVERREKGEIHARDFVTDPAIVIYNPDQLIATLTDDVPFSAELTVARGRGYVPASELI
                     DDNTEIGVIPIDALYSPVTRVRYRTEETRVGQKVNYDRLVLEIWTKGTVRPEHALVEA
                     AKILRKHLNPFVQYYDLGQIQTAGEARITPPPPVDDEVRRRLDRPIADLELSVRASNC
                     LESAKVTTIGDLVRLTEEELLQLRSFGKTSLMEVKRKLAEHGLALGMDVSGQSSGPGL
                     NPVSAGASGVQI"
BASE COUNT         1118 a         2143 c         2302 g         1390 t
ORIGIN      
        1 catacgggca gcagcgtcgc tggccgccgt accgccgggt gagctgcctg acaaattgcg
       61 ccaggacctg gagcgggcga ccgaagagct gctggccgtc agccgcgcac ggccggacga
      121 ctcggcgtcg caccacaatc tgggcaactt tcacgcggcg cggcgggaat atgacctggc
      181 gctgtcctgc tacgagcggg cgtttcagct cctgccggac aatatcgcgc cgctggtaaa
      241 tgcgtcgctg gttcagaatg ctctcgggcg aaacgatcag gccgaagcgt ctctgcgacg
      301 ggcgctgcag atcgagccgg gcagcgccgc cgcgaacttg aacctcgggc tgctgctcgg
      361 cgagatgggg cgtcttgcgg aagctgaagc cgtcctgcgg cgggcattcg aggctgatcc
      421 acaatcggct accgcggcct acaacctggc cgtcatctgc ggtcagacca acccggaagc
      481 gggcatcgac tggtgccgtg cggccgcaca gttgcagccg gacgagccga agtataggca
      541 tgctctggcg ttctacctgc gcaggggggg cgatattgaa gcggccatca cgacgctgcg
      601 cacgctgact gatcgggatg tggcctttgc ggacgcatac acgttgctgg gccagatcta
      661 cgaggaagct cgccgaatgg ccgacgccgc tgagctctac cgcagggcgg cggcgaatcc
      721 gcgctttccg aacgggctgc ggcagcagtt cgccgcccgc gccgcgcgcc tcagtcctgg
      781 gtgacactat ccagcgtcct tgctcctcac gcttcgaaat gcggctggga gtgtcatgct
      841 ccctgggccg gcgatggcca ggccgagggc gatgaaggcc agggtaaggg ggtactccat
      901 gccaccgtgc tgtgctgaga aagcgtggcg gtgaaccagc aggctggcga cggccatgtt
      961 gaagaagagc aaaaggctca tgacccgcat tccgattccc gttatcaagg ccaggccgcc
     1021 gaggaattca accagcgcgg cgatcgtggc cgccaccgca ggcgacggga ctccctggtt
     1081 cgccagaaac tcggcgaatt tggtcatgcc gctgccgccg aaccagccga acagcttctg
     1141 gctgccgtgg aacacaaaca caatgcccgg caacaggcgg attaggaaaa gccccacgtc
     1201 cagattagtg agtcccgcgg taaccgggct ggtgtctggg gtttcgggga tcgctgattc
     1261 ggcgctcatc ttcggctccg ttcacgcaaa tcttcgactc aattcgtgca aatcttcggc
     1321 tccgttcttg cgaatttggg ctccgttcat gcaaagcgga gctgtgcact cctctgtcac
     1381 tggggatgcg aagctggagc ttggcagtcc gctatccatt gggcgggcaa agccggagct
     1441 ctgcactccc ctatctcttg cagcgcgcga agccggacct ttgcactctt aagtctagcc
     1501 cacttgtgag tctattccac tcgccactct cagagtgtag tccaaccggc actccctgag
     1561 tcacctggga ctatctgagt ctagtccact tggaaaagcg gcgtcgataa atagcgctcg
     1621 ccggcggagg gaaatactgt cacgataagt ttgcccttgt tctccgggcg gcgcgccacc
     1681 tggtccgccg cacacagggc cgcgcccgag ctgataccgc agagaatgcc ctcttcgcga
     1741 gcgcagcggc gggcccagcc gaaggcttcg tcgtccgtga cggtgatgac gtcgtcgatg
     1801 atgtccaggt tgagaatctt gggaatgaac ccggccccga tgccctggat tttgtgcggc
     1861 ccgggcttca acggctgccc ggcgcgggcc tgcgtaatca ccggcgacgt cgccggctcc
     1921 acggcgatgc actggaagcc gggcttgcgc tgcttgatta cctcaccgac gccggtgatg
     1981 gtcccgcccg ttccgacgcc cgcgaccagg atgtcggccc ggccatcggt gtcgttccaa
     2041 atctcctcgg ccgtcgtccg ccggtgaatc tcgacgttgg caggattctc gaattgctgc
     2101 ggcatgtagg ctttgggcat ttcggccagc aactgttcgg cacgtgcgat cgcgccgcgc
     2161 attccctcag ccgcgggcgt gagaacggtt tctgcgccga gcgctcgcag gacggcccgg
     2221 cgttcgagcg tcatgctctc cggcatggtg aggatcaatc tcagccgttt tgcggcgcaa
     2281 acgaacgcga ggccgatgcc ggtgttgccg gacgtgggtt cgattacgac ggtctcgggc
     2341 gttaccaggc cgcgtgcgat ggcctcctcg atcatgctga cgccgatgcg gtctttgacg
     2401 cttttgagcg gattaaagta ttcgagcttg gcatagacgg cggccccgcc acgaacggcg
     2461 cggttaatgc gtacgagcgg cgtatttccg atgagtctgg cggagtcgtc gaataagcgg
     2521 ctcatggtgt gctcctaagc gctagtgcgc ggccgatatc agggccgatg aattgattct
     2581 ctggcgctgg ggcaacattt caatgagggg aggctcgcgc ggtggggaag ggatccaggg
     2641 atcgagggat cgagggacca agacggtggg acgcgggtgc gacgccccgc gcctcgggtt
     2701 ggggcagtca ggcggggggc ggccacgggc tcagtcaagt gggcatgccc gcgctcggcg
     2761 cttgggcatg gcacccggcg ttttgtgggg cactcggtgg gggcatgccc gcgccgctac
     2821 gcggcttggg catggcaccc gaccaccgac gcacgttttt cggcgttttt agtaggcccg
     2881 aagagggcgg gccctacttt ccgggtgccg gctgggcctt gctgggaacg actgtgcggc
     2941 ccgtggcgcg tttcgtggtt ggttgcgact gcgaacgcct gcggccttcg cggcgttcaa
     3001 ccggggcttc aagcttggcg aacttcggat cggcgtcgtc agtctcgatg atgatcttcg
     3061 caccgtcaac cgggatttcc tcgaacggcg gcagctttac cttgatctgg taataaccca
     3121 gaatcgcgcc ttccgccggc ggcgagtggg gcatcagttc acactggatg gtctccaggt
     3181 cgcagtgcac gcccaagatt ttcaacggct tgtcggcacg atactgcacg ttaagcaccg
     3241 tctcccatgg ttccttccgg gacggcgtga catacaccgc atagggcgag agctgtgcgc
     3301 gcggcataac gttggccgag agccgaagtt cgatgtccgg caagtcgggc cggccggttt
     3361 ccatggccag gaccgtcatg ttcttgccct ggttcagcgg cggcttggtc gtgacagtca
     3421 gctcgtagag cttgcccgct tcgacttcct tcaactcgta ctcgaattgc ttgatattct
     3481 ggccgggctt gatgcgcagg gggagcggct cggggtattg gttctcaagg cggatggttt
     3541 gcgtggcgac cgtgttttct tcggcgttgg cgaaattgca atcgggcggc gtggcggcga
     3601 agatcggctt gacggtgccg ttgacgtcaa tcgtgatagt cggctgcgtc ggatcgttcg
     3661 agttgatcat gacctttttg ccggccgggc cgatccgccg tgtgtcgtac gtgatcgtga
     3721 acacgtcgct ctcgccgggc tgcagcgggg acttgggctt ggtcgccacc gtgcagccac
     3781 aagagctggt cacggttgtg gtgagcgggg tatcgccggt gttctggatg tgaaattcgc
     3841 ccttggcggc ggcgccctgc cagacctcgc cgaaatcgaa ggcgaccggg cgtagcaaga
     3901 tgcgtgcacc cacgtcgctg accggctggc tagccggggc agtttgcggg gcaggcggcg
     3961 cggcgggctg ctgagcggcg gcgacagaaa acgcggttag tcccaggaaa aacgccgaga
     4021 aagtaaacgc tgacttcata tctactgcct ccaagcgtga caccggccgg acgccggcgc
     4081 cacggtgcca ttgcttcaac cgcaccagcc ggcggcacag gccacaggct ggatatttgg
     4141 acaccctgcg gcatcgatgg ttggggcccg agcctttgct cagaaaccgg ctggaaaccg
     4201 gtcgcacaac gttacataga aagggcccgc gccttgcggg cggacccttg atggagacga
     4261 aggggctcga accctcaacc tccgcattgc gaacgcggcg ctctcccagt tgagctacgt
     4321 ccccgagcac cccaattata aatcaggttg gccgccgtgc caaacggtcc gggaggcaat
     4381 tggcgacatt ccggggcgat gcgcgccggc tgctcgcctc gtctcgacct cgctggctgc
     4441 ggcccgcttt ggctccgctg cggcccgcac tgcttgaagc tgcgctttca agcagtggca
     4501 cacacgcgtg tttacaccgc gtggcggtca taggcggctg gttatggcgg ccgccatttt
     4561 ttccaattcc gcaggggcgg ccttcttggc cttccagcgg tatcccagtc cgtcgccagc
     4621 gtggcgcggg atcaggtgaa agtgcacgtg cgggacttcc tgttccgctg ccctgccgtt
     4681 gttctgcagc acgttgaaac cgtcggcgcc gctggcgttc acgacggctc ggccgaggcg
     4741 ccccaaggtt cggccaatag ccaccgccca atcttccggc agttggtcca agaattccgc
     4801 atgatggcgg ggtatcacga ccgtgtggcc gggcgccaag ggctggatat ccaaaaaggc
     4861 caggacgtgt ttgtcttgat agacacgctg gcacgggatt tcgccgcgga tgattttgca
     4921 gaagacgcag ttttgatcca ggacggcgtt ggccatggct agccctctgg gacctgctcc
     4981 ggggcggcac gccccggctc tgctattaaa ttttactgca ttctccgggc gacacgtccc
     5041 ggctctgctt ctgggattct attgcacgct gcgggcgaaa cgccccccgg ctccgagctt
     5101 aattttttgc actttccgag ctttattgca ttctccgggg cggcacgccc cggctctgct
     5161 cggtctttat tgctcggctt tttcctctgc gccgggttcg ggctgggccg cgcttactcc
     5221 ggcctgcggc ttcgcttcgg cctccttcga cgggcgagtg ctgcgccgtg gagcgcggcg
     5281 cggggcggcc ttgccggcaa aggcggcgta tttggcacgg gcgcggcgct tgcgctccgt
     5341 cttctccgtg ctcttggggc gcttcttgtc gtcctcgccc acccattgca gcaaggcggt
     5401 gtccgacccg tcgcccaggc ggcgctgcgc cagcttgatg acgcgcgtgt agccgccgga
     5461 gctgcccagg gcttcgttgc gctttttgaa gcgcggaccg agttctgcga acagcttgtg
     5521 gatgaccgaa tcggccgtga acttcacgcc gggcttggta gtgctggccc gatagcggcg
     5581 gccggagcgc gcccgcagga ccttggcgcg tttggcatcg ctcattttgt cgtattcttc
     5641 ctgctgctcc ttggcgatga tggcgcgatc gttgagcaaa gcgatggcgc gctgccgcgc
     5701 ggccaggctg ccgtcaatgg ccagggtggc cagacgctca gcaaagcgtt gcacttcctt
     5761 ggccttgggc agggtggtac gaatctcgcc gtgctctatc aggctctgca ctaaattacg
     5821 ccgcagcgcc cggcgatggg cggtcgtgcg gttcagtttt cgtccggcaa cattatgtcg
     5881 catgcctacc tcaaatctgc acacccgacg cgccggccga gaccgggttc aacccaggcc
     5941 ccgacgactg gccggatacg tccatcccca gcgccaagcc gtgctcagcg agcttgcgct
     6001 tgacctccat gagcgaggtt ttgccgaagc tgcgcagttg cagcagttct tcttccgtca
     6061 ggcgcaccaa gtccccgatg gttgtgacct tggccgattc caggcagttg ctggcccgaa
     6121 ccgagagttc caggtcggca atcgggcggt cgagccggcg ccgcacctcg tcatccacgg
     6181 ggggcggcgg cgtgatgcgc gcctcgcccg cggtctgaat ctggcccaaa tcgtagtact
     6241 gcacgaacgg gttgaggtgc ttgcgcagga ttttggcggc ttcgaccagg gcatgctccg
     6301 gccggacggt ccccttggtc cagatttcca ggaccaggcg atcgtagttg accttctggc
     6361 cgacgcgcgt ttcctcggtg cggtagcgga cgcgcgtgac gggcgagtag agagcgtcga
     6421 ttgggattac gccgatctcg gtgttatcgt cgatcagttc gctggccgga acgtacccgc
     6481 ggccgcgagc gacggtcagc tcggccgaga atggcacgtc gtcggtcaag gttgcgataa
     6541 gctgatcggg gttgtagatg acgatggcgg gatcggtcac gaagtcgcgg gcgtggattt
     6601 cgcccttttc gcggcgttcg acgcgcatcg tcttcggcgt ctccgagtcg aggtcgacca
     6661 cgagcgcctt catgttaagg atgatgtcgg tcacatcttc gagcacgccg ggcagcgagg
     6721 tgaactcatg ttcggcgccg gcgatgcggg cgtgcgtgac ggccgaaccc tccaggctgg
     6781 acagcagaat tcgccgcaag ctgttgccga ccgtcgtacc aaatccgcgt tcgaagggct
     6841 ctaccgtgaa caacccgtac gtgtccgtgc tgagcgcctc gtcgcgcacg acacgcgtgg
     6901 gcagttccag gccccgccat ctgatccgca tttgctcatg cctcccgaga ggt
//
LOCUS       DAOSYS010000190         6935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9260_length_6935_cov_5.344041, whole genome shotgun sequence.
ACCESSION   DAOSYS010000190 DAOSYS010000000
VERSION     DAOSYS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9260_length_6935_cov_5.344041"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(546..776)
                     /locus_tag="PLU99_11515"
     CDS             complement(546..776)
                     /locus_tag="PLU99_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17721.1"
                     /translation="MTSSLPVEMLQILELLPPGKHLPWWELHRRHEDVFGIDTRPATF
                     RQEIATLHSLGLLRQHGEYEFGLADGVKALRH"
     gene            complement(867..1886)
                     /locus_tag="PLU99_11520"
     CDS             complement(867..1886)
                     /locus_tag="PLU99_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17722.1"
                     /translation="MTRNRNAFEGCNGDGVVDQVIPAQQQETLEDEAGRESDRRRYRI
                     IKTLFENHESGRITIRELERNRKFTRAFRHRGATTLEEDFRRIEALLGNGQPFRVTRD
                     GAAMLRWVPAGESAFAELGYCSAASAPPKLAAETEDKRRIGELVVSELLNADVEVLYL
                     STGTTVYQVALALLNSERENVSMILTDNAAIVELCCQRSMTDRRLRENKLLLHLIGGR
                     LSFNPPDIESESEFKEFKRWKCSKAVISVTGINPETGQLYSFRLPKTKEEVLLDLDVR
                     EVIIPLLPNKIGQAGGRLIHDPRNQNHENRIYRIVTTKLAPGVKAQLEKHRYEVREVD
                     APRPT"
     gene            2419..4716
                     /locus_tag="PLU99_11525"
     CDS             2419..4716
                     /locus_tag="PLU99_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17723.1"
                     /translation="MKLVAAAAPLWLRLILPLVVLGVTFAAFAPALNNEFLNWDDDKV
                     LLNNDRFRGLGSEQLKWAFDAYFMGHYHPLTWISYGIDYKIWGIDNAFGYHLTNLVLH
                     ALNAVLFYLLAARLLSLTRGITGRPPPAALAVAAVAALLFAVHPLRVESVAWATERRD
                     VLSVFFLLACVICYVRYAAGGAGSWRWYVASLLLLLASLLSKAWGMTLPVILLLLDFY
                     PLRRVSLSIESLSRPGAVRVLLDKMPFVPLAAWAAVMAGRAQAAAPDTMKTLAEHGVL
                     QRIAQAFYGLGFYVWKTLVPIGLGPLYEIPPQMSPFEPRFILAALLVVPGIVLLLAAR
                     RCRPAGPVVLAIYVITLMPVLGFAQSGPQLVADRYSYVACMPLAVLAGSGLWWGARYL
                     SQRWSRVPALVLSGAVSAAVIAGLAVLTWRQTFVWHDSRTLWEHNLTICPGSPNSHHN
                     LGVCLANERLYEQAIEEFKQTLSLTPRHATALYSLGRALHLLGRTDESIQYFNAALAI
                     KPNEAKAHLWLADALRDLGRLDEAAQHSSAAARLNPTGAAIADASPEAAAARLAAVVQ
                     ANPNDANARYELGMVLGRMQRVAEAAQQLSECVRLAPDHFEARCALAASLASLGNVPA
                     AAAEYQQALAQRPDSAEAHGRLADLLAMAGDEAAAVSHYRAALQLAPDRWGSANNLAG
                     LLATSRNPAVRDPQEAVRLAEQACVGTKFSQPGFLTTLAIACVEAGQVEQVLQRLPNA
                     AVTADLQQRIAAYQQQRAAAAQSNP"
     gene            complement(4706..6166)
                     /locus_tag="PLU99_11530"
     CDS             complement(4706..6166)
                     /locus_tag="PLU99_11530"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOO17724.1"
                     /translation="MSDAYTGPLERIDECRWRIPRTAKAGMRVDGIIYADDTLMQSIR
                     QDKSPEQVANVATLPGIVRASLAMPDIHWGYGFCIGGVAATDPQEGGVISPGGVGYDI
                     NCGVRLVRTDIPAEDCRPRLKKLVDTLFSTVPCGVGGTGKIKFTRSEMKELVVEGAPY
                     VVKRGYGTDEDIERTEAGGQLEGARPGFVSDHAYERGHDQVGTLGSGNHFLEVQIVDE
                     IVDPHAAEVLGLKLGAITVLIHSGSRGFGYQICEDALKDLRHAPAKYGIELPDRQLVC
                     APVHSDEGQRYLGAMRAAANYAWANRQLLLHLARQAFMRVFELPSQKLGMRLVYDVAH
                     NIAKMEPYKINGETRMLCVHRKGATRAFPPGHPEVPAVYREVGQPVLVPGDMGRSSWV
                     LVGAPGAMEETFGSTCHGAGRVLSRSAAIRAAKGRSIEKELLEAGVIARARGRTGLAE
                     EQPAAYKDVSEVVDVVHKAGLAKKVARLKPIGVIKG"
     gene            complement(6204..6629)
                     /locus_tag="PLU99_11535"
     CDS             complement(6204..6629)
                     /locus_tag="PLU99_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOO17725.1"
                     /translation="MQARYELFDHTADVGVRVYAPTLAGLIEPATRGLYEVIGELAGK
                     TGAPAEQVRLEFSGDEPAVMLRDYLAEVLRRFETCHQLATEVEVEQFTEDRLAVSVKM
                     RPVDPHRSKYANEVKAVTYHELGIRSSPDGFEATYIVDI"
BASE COUNT         1160 a         2100 c         2276 g         1399 t
ORIGIN      
        1 ctccgggcgg gaacagttca ctgtccggtg ggaacgtggg aaggtgggaa agtgggaagg
       61 tgggaatgtg gccgtagcgg ccggcgtggc gatgattgat ggatagggcg aagagttttt
      121 ggttgttagt tgttagtcct gctggaccgc tcgacgcgga cgcacccgtg ggaacgtggg
      181 aaggtgggaa tgtgggaacg tggccggagc ggccgacgcg gcgatgaatt ttgatcagtg
      241 gcggtgaatt ttggtcagtg gcattaaacg atcagtcgca atgaattttg atcagtggca
      301 atgaactttt ggtcagtcgc gatgagtttt tatcagtacc cgcggcggcc tggatagagg
      361 ttcagagccg cgtgtgcgcc ggggtggcag caaaggttcg gcgcaaggcc ggcaaaagtt
      421 cggcagaaac ccaggcaaaa gcccgacagc agcccggcaa aggctcgaca caggccgggc
      481 aaagctcgac agaggcccgg cagacgctcg acaggggccg ggcaaagggg cggcgacggc
      541 ccggctcaat ggcgcagcgc cttgacgccg tcagccaacc cgaactcata ctcgccgtgc
      601 tgccgcaaca accccagcga atgcagcgtg gcgatttcct ggcggaacgt ggccggtcgc
      661 gtgtcgatgc cgaagacgtc ctcatgccgg cggtgcagct cccaccaggg caagtgcttc
      721 ccgggtggca gcagttccag gatctgcagc atttcaacag gaagggaact tgtcatttgg
      781 ttggggttca tgtctaccct cgcaaaacgg gtttcgtttc tcctggtatc tttcgtcatt
      841 gaagatcggc gagagcccgg cggcctctaa gtgggccgcg gagcatccac ctcccgaact
      901 tcgtaacggt gtttctccag ttgggcctta accccgggag caagtttagt cgtcacaatt
      961 cgataaatgc ggttttcgtg gttttggtta cgcgggtcat gaatcaggcg cccgcccgcc
     1021 tgcccgattt tgttgggcag aagagggatg atcacttcgc gcacatccag atcgagcaac
     1081 acctcttcct tggtttttgg caggcggaaa ctgtaaagct ggccagtctc cgggttgatt
     1141 ccggtcaccg aaatcacggc tttggaacac ttccagcgtt taaactcctt gaattcagac
     1201 tcactttcaa tgtcgggcgg attgaaagag agccgcccgc cgatcaggtg aagcagcagc
     1261 ttgttctcgc gcagtcgcct atcggtcatc gagcgctgac agcacagctc aacgatggcc
     1321 gcattgtcgg tcagaatcat tgagacgttt tcgcgctcgc tgttcagcaa ggccagtgca
     1381 acctggtaca ccgtagtgcc ggtgctcagg taaagcacct cgacgtctgc attgagcagt
     1441 tcacttacga ccagttcccc aatgcgccgc ttgtcttctg tttcggccgc gagcttggga
     1501 ggcgccgaag cggccgagca gtagcccaac tctgcgaagg ctgactcgcc ggccggcacc
     1561 cacctgagca ttgcggcgcc atcgcgtgtg acgcggaagg gctgcccatt tcccaagagt
     1621 gcctcgatcc ggcgaaaatc ctcctcgagc gtggtcgcgc cgcgatgacg aaaggcgcgc
     1681 gtgaacttgc ggtttcgctc aagctcgcgg attgtgattc gtccagattc gtggttttcg
     1741 aaaagcgttt tgatgatgcg gtagcgacgg cgatcggact cgcggccggc ttcatcttcg
     1801 agtgtttctt gttgttgggc tggaattact tgatcgacca ccccgtcccc gttgcagcct
     1861 tcaaacgcgt tccggttccg cgtcatttca gccaatcctt cgcgaggtgg cagcgtccgc
     1921 gcaggacact gctcaaatgt cataataaca tcatcgtgcc aattttgcta gccctgtaac
     1981 caaatttttg tccgacgttt ctagtctaaa gccaaatttg gtagagccat agccataatc
     2041 tcggcacatt gagccattta cctgagcagc gcctgtcgcc agagcagcag actaaggcca
     2101 agatcggtgg ctcagggcgc gtttgcctgg aaacgttttg cccaaaccag gtaaaaacac
     2161 ggcctgatcg cgcgccacgt cgcgtacggc gcggccgccc agcgcgcggc tggtgacggc
     2221 cggcagtctc cgctaaaatg gctgtttccg atggtaaatg ttgcgcccaa cagaaagaat
     2281 tcggcaaccg gtgagcgcgg cggcgggcgt ggagctgccg atcctgcgaa acgcagcgaa
     2341 acgcgctccc gagcccttcc cgatcgcaat ctccgccgca aggtggccga tggctttccc
     2401 ccgagtggtg agccggaggt gaaactggtt gcggccgctg ccccgctgtg gctgcggttg
     2461 atcctgccgc tggtcgtcct gggcgtgacg ttcgccgcct ttgccccggc gctgaacaac
     2521 gagttcctga actgggacga cgacaaggtt ttgctcaata acgaccgctt tcgcggactg
     2581 gggtctgagc agctaaagtg ggcttttgac gcctacttta tggggcacta tcaccccctc
     2641 acctggatca gctacgggat cgattacaag atttggggca tagataacgc ctttggttac
     2701 cacctgacga atcttgtgtt gcacgcgctg aacgccgtgc tcttctattt gctggcggcg
     2761 cggctcttga gcctgacacg ggggattacc ggacgcccac cgccggctgc gctggcggtg
     2821 gccgcggtgg ctgcgctgct ctttgccgtg cacccgctgc gggtcgagtc cgtggcctgg
     2881 gcgacggagc ggcgcgacgt actgtccgtt ttctttttgc tggcgtgcgt gatctgttac
     2941 gtgcgttatg ccgccggcgg cgcgggcagt tggcgctggt atgtcgcgtc gttgctgctg
     3001 ctgctggcat cgttgctgtc caaggcttgg ggcatgacgc tgccggtcat tctgctgctg
     3061 ctcgactttt atccgttgcg gcgcgtcagc ctttcgattg agtcgctgtc gcggcctggc
     3121 gcggtccgcg tgctgctgga caaaatgccc ttcgtcccgc tggcggcatg ggcggcggtc
     3181 atggcgggtc gggcgcaggc ggccgctccg gatacgatga agacgctggc cgagcatggc
     3241 gtgctgcaac gaatcgcgca ggccttctat gggctgggct tctacgtctg gaagacgctg
     3301 gttccgatcg ggctggggcc gctgtacgaa attccgccgc agatgagccc gttcgagccg
     3361 cgattcattc tggcggcgct gttggtggtg ccgggcatcg tgctactgct ggcggcgcgg
     3421 cgctgccgtc cggccgggcc cgtggtgctg gcgatctacg tgatcacgtt gatgccggtg
     3481 ctcgggttcg cgcaaagcgg accgcagtta gttgccgacc gctattcgta cgtcgcctgt
     3541 atgccgctgg cggttttggc gggcagtggg ttatggtggg gggcgcgcta tctgtcgcag
     3601 cgctggtcgc gcgtgccggc actggtcctg tctggcgcgg tctccgcggc agtgattgcc
     3661 ggcctggcgg ttctgacctg gcggcagacg ttcgtgtggc atgattcacg gacgctttgg
     3721 gaacacaacc tgacgatctg ccccggcagc cccaattcgc atcacaatct gggcgtgtgt
     3781 cttgcgaacg agcggctcta tgaacaggct attgaggagt tcaagcagac gctgagtctg
     3841 accccccgcc acgccaccgc cctctatagt cttgggcgag ccttgcacct gctggggcgg
     3901 accgacgaat ccatccagta cttcaatgcc gcactggcca tcaagcccaa cgaggccaag
     3961 gcccacctgt ggctggctga cgccctgcgc gacctgggcc ggcttgatga ggccgctcag
     4021 cactctagcg cagccgcgcg gctcaatccg accggggccg ccatagctga cgccagtccg
     4081 gaggcggcgg cggcgcggct ggcggcggtg gtgcaggcga accccaacga tgccaacgcg
     4141 aggtacgagc tggggatggt gctcgggcga atgcagcgcg tggcggaagc ggcgcagcaa
     4201 ctatcggagt gtgtgcggct ggcgccggac catttcgagg cccgctgtgc gctggcggca
     4261 tcgctggcct cgctcggtaa cgtcccggcc gcggctgccg agtatcagca ggcgctggcg
     4321 cagcgtccgg attcggccga ggcgcatggc cgcctggccg atctgctggc gatggcgggc
     4381 gacgaggctg cggcggtcag ccattaccgc gccgcactgc aactggcgcc cgaccgctgg
     4441 ggttcggcca acaacttggc gggattgctg gccacgagta ggaacccggc cgtccgcgac
     4501 ccgcaggaag cggttcgcct ggcggaacag gcgtgtgtgg gcaccaaatt cagtcagccc
     4561 ggtttcctga cgacgctggc aatcgcgtgc gtcgaggccg ggcaggttga gcaggtcctg
     4621 caacggctgc cgaacgcggc agttacggcg gatttgcagc agcgaatcgc ggcgtaccag
     4681 caacagcgcg cggcggccgc ccagtctaac ccttgatgac tccgatcggc ttgaggcgtg
     4741 ccaccttctt ggccaagccg gccttgtgga cgacgtcgac cacttcgctg acgtccttgt
     4801 aggccgcagg ctgctcctcg gcgaggccgg tgcggccgcg ggcgcgggcg atgacgcctg
     4861 cttcgagcaa ttccttctcg atcgagcggc ccttggcggc ccggattgcc gcgctgcgcg
     4921 acagcacgcg ccctgctccg tggcaggtgc tgccgaaggt ttcttccatg gcgcccggcg
     4981 cgccgaccag cacccagctt gagcgtccca tgtcgcccgg caccagcaca ggctggccaa
     5041 cttcgcgata gacggccggc acctcggggt ggccgggtgg aaaggcgcgc gtggcgccct
     5101 tgcgatggac gcaaagcatg cgtgtttcgc cgttgatctt gtagggctcc atcttcgcga
     5161 tgttgtgggc cacatcgtaa accaggcgca tgcccagctt ctgcgacggc agctcgaaaa
     5221 ccctcatgaa cgcctgccgc gccaggtgca gcaggagctg gcgattggcc caggcataat
     5281 tcgcggccgc acgcatggca cccagatacc gttggccctc gtcggagtgc accggtgcgc
     5341 agacgagttg gcggtcgggc agctcgatgc cgtacttggc cggcgcatgc cgcaggtctt
     5401 tgagcgcgtc ttcgcagatc tggtagccga agccgcgcga gcctgagtga atcaggacgg
     5461 ttatggcgcc cagcttcagc ccgagcacct cggccgcgtg cggatccacg atttcgtcga
     5521 ctatctgtac ttcgagaaag tgattgccgc tgccgagcgt gccaacctgg tcgtgcccgc
     5581 gttcataggc gtggtcggag acgaaacccg gccgcgcgcc ttccagttgg ccgccggctt
     5641 cggtgcgctc gatgtcctcg tcggtgccgt agccgcgttt caccacgtat ggcgcgcctt
     5701 cgacgaccag ctctttcatt tcgctgcgcg tgaactttat tttgccggtg ccgcccacgc
     5761 cgcagggcac ggtggaaaag agcgtgtcca ccagcttttt caggcgcggc cggcagtctt
     5821 cggcggggat gtcggtgcgc accaggcgca cgccgcaatt gatgtcgtac cccacgccgc
     5881 cggggctgat tacgccgcct tcctgcgggt cggtggctgc cacgccgccg atgcagaagc
     5941 cgtagcccca gtggatatcc ggcatggcca acgaggcgcg gacgatgccg ggcagcgtgg
     6001 cgacgttggc gacttgctcg ggtgatttgt cctggcgaat ggactgcatg agcgtgtcgt
     6061 cggcgtagat gatgccgtcg acgcgcatgc cggctttggc ggtgcgcgga atgcgccagc
     6121 ggcactcgtc gatgcgttcg agcggtccgg tgtatgcgtc agacattttg aacctcagac
     6181 cagatggcag cagatagtgc tattcatata tcgacgatat acgtggcttc gaatccgtcc
     6241 ggcgacgagc gtattcccag ctcgtggtaa gtcaccgctt tgacctcgtt ggcgtattta
     6301 ctgcgatgcg gatcaactgg ccgcattttc acggatacgg ccagccggtc ctcagtaaac
     6361 tgctcgactt cgacttcggt cgcgagctga tggcacgttt cgaagcgccg cagaacctcg
     6421 gccagataat cccgcagcat tacggcgggc tcatcaccgc tgaactcaag ccgcacctgc
     6481 tcggcgggcg cgccggtttt gcccgcaagc tcaccgatga cttcgtacaa accgcgcgtg
     6541 gccggctcta taaggccggc cagcgtcggc gcgtacacgc gcacaccaac gtcggccgtg
     6601 tgatcgaata gctcgtaacg cgcttgcatc gtacaccctg cgggaccggt tttagcgagc
     6661 ttgcgaggtg gacctgtcag ccggttccgt agcggctcgg catgcaggac cggtctgcgg
     6721 ccggtcctgc acggtttgct cataaagcct ggtcgagcta cgccatccgg ctgagttggt
     6781 cgcggatgac ggcttcgaag ccgcctttta ccaccagctc ctgcgccacc ggacccagcg
     6841 ccgggaatga gtatatccgc tcggacccgc cggcgctttg cactcgaatt tgcgagcgcg
     6901 tgaagtcgat ggtggtctgc cagccggtgc ggatg
//
LOCUS       DAOSYS010000191         6910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9311_length_6910_cov_5.185850, whole genome shotgun sequence.
ACCESSION   DAOSYS010000191 DAOSYS010000000
VERSION     DAOSYS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9311_length_6910_cov_5.185850"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1086)
                     /locus_tag="PLU99_11540"
     CDS             complement(85..1086)
                     /locus_tag="PLU99_11540"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOO17726.1"
                     /translation="MSDAPISTPTLDVDLAGLSLRNPVLTASGTCGYGPELADVLDYT
                     RLGGFTTKSVTREERLGNEPQRLVEVRGGLLNAIGLANVGLKRFVAEKIPYLQQMPTT
                     VLVNVAGHSIEDYVTVCGTLDAYPCIAGLELNVSCPNVSDGLMFGTDPRLLKTLVSAV
                     RPHVRRAKLIVKLSPNVTDIALTARAAIDGGADILSLINTYVGMSIDVVTRRPRLANT
                     TGGLSGPGIKPLAVYAVHRVYRQVARAAGIPLIGMGGVQTTEDALELFLAGASAVAVG
                     TALFVDPATPLKIADGLCAYMQQHGISRISEVTGAVELPAPAAKPATSPNGLGGAQG"
     gene            1239..2366
                     /locus_tag="PLU99_11545"
     CDS             1239..2366
                     /locus_tag="PLU99_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17727.1"
                     /translation="MASRWVDKLYDWLAKLPSPDAEMILSAGLERAEPPYFERLVELL
                     FERASEAAWAELIANYPRLSPQARARLRADGQQMRAGLARVLRGGSPLGRCNALQALC
                     DDPAPALAYLLPDVLRTSSGDVRRMAAETLRWLAEFVLDFAKPTEQAGPARAEPENRS
                     RGESRSEAVARQRADRASVARAVCEALRGFESHYRVEVVEAALWLTRDLGEDLWSVLA
                     NRRLPTAHVVSANLKAWDGPRLARFLLEALAQRSWRASAREQLMRWQGPERLAALLRN
                     SDLLERPDFREGLNTLGRPGWFFGLNDLRDLPPELRARAPEWLVLLGYRPEQKRALLA
                     HWSEVAFEELRVAAEAAARAMAQGPECWAVDASTLPREDGA"
     gene            2363..2860
                     /locus_tag="PLU99_11550"
     CDS             2363..2860
                     /locus_tag="PLU99_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17728.1"
                     /translation="MTWAWLMLAQGAPLTRRQALELLRKGFHRELASNDLFIYVIGAV
                     ALIALILLLSQVISQRQRPKTPPRRDYLLEAVKVLGLTRQERSDLLELAARARLAQPA
                     AMLLSPANLSYALSRAAIDAPAGPLRNRLDALSRKLFGDGLARDTGHSTFQTASRTAS
                     GNQPV"
     gene            complement(2818..3618)
                     /locus_tag="PLU99_11555"
     CDS             complement(2818..3618)
                     /locus_tag="PLU99_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17729.1"
                     /translation="MILSIIAIVGLTCSASIAEEMSPERQRQVLREAQGAFDQAVELA
                     HSQPERSQELYRQAVAGFSALVEAGVRNAALEYNLGNAYFRLNELGAAILHYARAQLL
                     DPADQKIAANLEYARRQVEPAIERSGQTRLVRSLLFLHYDTALNGRFWAAVILSVAGW
                     FLLTLRLRWPRRELVVTGGLAVGLALSFAASVAWQVREDARHPRAVLVGGPTILRLGR
                     GEGYDPALQQPLGPGVEMRVLQQRGDWAEVRLPNDQTGWLPLAVLEAV"
     gene            complement(3624..5366)
                     /locus_tag="PLU99_11560"
     CDS             complement(3624..5366)
                     /locus_tag="PLU99_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOO17730.1"
                     /translation="MCVILLLLGAGAVPGFAKASVSLEIEPDEIYANEPFTVSIRVED
                     FSRCEQPDFPDLPQAIVRRVEGGGESMFTTIVNGRTTVKRSRTYTFHVTPQEPGELTI
                     PPIKLVVDGRTVQTEPVQVKVHPSDAQELLWAEITCDRQRIYVGQRVRLVMTIYVKPL
                     QVQGYRISSQDMMNFLIAVNFGPFPTNNIERDTRKLDRPDGGKETYFTYRTITDYVPD
                     RPGLLNFDDIQVGMRYPVRLTRDIFGRLEATAYRTLIATPKPSDVSVLALPTQGRPAN
                     FTGAVGTFDLTVLAEPTAVRVGDPIKLTMEIRGDGPLETLPPPNLAADPKLTGGFRVP
                     QEELAGEVVGGRKRFTQVIRAERSDLTEIPRLEYPYFDPDREMYVVALSEAIPLKVMP
                     AAELNTSALVERMDPPAANNQTLHALDGLHGNETREARLLASTAPVSLNALALATIGP
                     PAGYLIAWVCLSYARAGGSIRHRRQKALRQARARLAQAQKLPARQAAAEITAALTGYL
                     AERLDEPPARFIGPAAAGFLAQRNASASVREQWQSIVERCEQLSFGGLGTDTPTALAE
                     SADQCLRALERERL"
     gene            complement(5476..6555)
                     /locus_tag="PLU99_11565"
     CDS             complement(5476..6555)
                     /locus_tag="PLU99_11565"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOO17731.1"
                     /translation="MPRHAIYLLRAPCAPLLALLPLVLSGCADFGYIIRQAAGQLEML
                     VNSEPIDEVLAGGRLTPEQERKLRLVVEVRDFAASELGLNVGRSYRLYHEADGKPIAY
                     NLSACRKDSLSPKQWRMPIVGTIDYLGYFAKADAEKAAAQLEAQGYDTFIYGVDAYST
                     LGWLPDPVQSTFLNRADGNLVETVIHELAHNTIYVSGQSDFNESLATFIGRQGARLFL
                     EQRGPEGAAIAADLQAQYADDELVTDWVLKLNTDLRTYYASDLPSEAKIGGREAVFQA
                     ARDAFTTEVAPKLQLPERSAGWGRIPTNNAYVRLHQRYHVNLDAFAAALEAVNNDFRA
                     FLDIVQAAADTADPFAAIRAAAGSP"
     gene            6636..>6910
                     /locus_tag="PLU99_11570"
     CDS             6636..>6910
                     /locus_tag="PLU99_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17732.1"
                     /translation="MTEEKPVALPTLVSLLICDQVIDDKLTNKKSAIGMFNTVLVPQA
                     PTTLAQVMVLASLTDIKGRVVLRLRLVRDTDNEILFETHGPIESPDPL"
BASE COUNT         1167 a         2171 c         2332 g         1240 t
ORIGIN      
        1 agccggggcg tgtcgccccg gagtgacgcg gcaaaccatc atcatcgcag ccagcgtcgt
       61 ttcgtcgccg caggcagcgt catttcaccc ctgcgcgccg cccaaaccgt tgggcgaagt
      121 cgccggcttg gcagccggtg cgggcagttc gacggcgccc gtcacttcac tgatccgcga
      181 tattccgtgc tgttgcatgt atgcacacag gccgtccgca atcttcagcg gcgtcgccgg
      241 atcgacgaat agggcggtgc ccaccgcgac tgccgaggcg ccggccagga aaagttccaa
      301 cgcgtcttct gtcgtctgga caccacccat gccgatcagc ggaattcccg ccgcgcgggc
      361 cacctggcga tatacgcgat gcacggcgta cacggccagc ggcttgattc ccgggccgct
      421 caggccgcca gtggtgttgg ccagacgcgg gcggcgggta acaacgtcga tgctcatgcc
      481 gacataagtg ttgatgagac tgagaatgtc cgcgccgcca tcgatggccg cccgggccgt
      541 cagagcgata tccgtcacgt tcggcgagag tttgacgatg agtttggcgc ggcggacatg
      601 cgggcgcacg gctgacacga gcgtcttgag cagccgcggg tcagtgccga acatcaggcc
      661 gtccgagacg ttagggcacg agacgttcaa ctccagcccg gcgatgcagg ggtaagcgtc
      721 gagcgtgccg cagacggtga cgtaatcttc gattgaatgt ccggccacgt tgaccagcac
      781 cgtcgtgggc atttgttgca agtacgggat cttctccgcc acgaagcgtt tgaggccgac
      841 gttcgccaaa ccgatggcgt tcagcaggcc gccgcgaact tcgaccagcc gttgcggctc
      901 atttcccagg cgttcttcgc gggtcacgct tttggtggtg aacccaccca gccgggtgta
      961 gtcgagcacg tcggccagtt cgggcccgta gccgcaggtt ccggaggcag tcaacaccgg
     1021 gttgcgcaag gacaagccgg cgaggtcaac gtcgagggtc ggggtggaga taggggcgtc
     1081 gctcatggtt ggtggattgt atgtttttcg gaccgtcgcg acgagcggaa atggggctgg
     1141 cgaacctggc ggcgggtcga tacggcgacc gcgcgcaccg gtcgattttc ctattgatac
     1201 tgcgcctggg cccttggtca cgagaaggac atgcgctggt ggcaagccgc tgggtagaca
     1261 aactgtacga ctggctggcg aaactgccgt ctccggacgc ggaaatgatc ttgtcggccg
     1321 ggctggagcg agccgaaccg ccctacttcg agcgtctggt ggagctgtta ttcgagcgtg
     1381 cgagcgaagc cgcctgggcg gaattgattg ccaactatcc gcgcttgagc ccgcaagctc
     1441 gggcgcgact gcgggccgac gggcagcaaa tgcgggccgg gctggcacgt gtgctgcggg
     1501 gcggctcgcc gcttggtcgc tgcaatgcgc tgcaggcgct gtgcgatgac cccgcgccgg
     1561 cgctggccta tttgctgccg gacgtgctgc gaacttcctc cggcgacgtg cggcgcatgg
     1621 cggccgagac attacggtgg ctggcggagt tcgttcttga ttttgcgaag cctaccgagc
     1681 aagcggggcc agctcgtgcc gagccggaga atcgtagtag gggtgaatct cggtcggagg
     1741 ccgttgcgcg gcagcgcgcc gaccgggctt cagtggcgcg ggcggtgtgc gaggccctgc
     1801 gcggcttcga atcgcactac cgcgtggaag tagtcgaagc ggcgctgtgg ctgacgcgcg
     1861 acttgggcga agatttgtgg agtgtgctcg caaaccgccg cctcccgacc gcccacgtcg
     1921 taagcgccaa cctgaaggcc tgggacggcc cgcggctggc ccgatttctc ctggaggccc
     1981 tggcgcagcg gagctggcgc gcctcggcgc gcgagcagct catgcgctgg cagggcccgg
     2041 agcgcctggc agcgttgctc agaaattcgg acctgctgga gcgccccgat ttccgcgaag
     2101 gcttgaacac actcgggcgc ccggggtggt ttttcggcct gaacgatttg cgcgacctgc
     2161 cgcccgaatt gcgcgcccga gcccccgaat ggttggtcct gcttggctac cggccggagc
     2221 agaagcgcgc cctgctggct cattggtccg aggtcgcgtt tgaggagttg cgcgtcgcgg
     2281 ccgaagcagc cgcgcgcgcc atggcgcaag ggccggagtg ctgggcggtt gacgcgagca
     2341 ctctgccgcg ggaggacggg gcatgacctg ggcgtggctg atgctggcac agggcgcgcc
     2401 gctgacacgg cgccaggcac tcgagctgct gcgtaagggc ttccacaggg agctggccag
     2461 caacgacttg ttcatatatg tcatcggcgc ggtcgcgctg atcgcgctca ttctgctgct
     2521 gagccaggtg atcagtcagc gtcagcggcc aaaaactccg ccgcggcgcg attacttgct
     2581 agaggcggta aaggtgctcg ggctgacacg ccaggagcgc tcggacctgt tggagcttgc
     2641 ggcgcgggcg cgcctggcgc aaccggccgc catgctgctt tcgcccgcca acctgagcta
     2701 cgccttgagc cgggccgcga tcgacgctcc cgccggcccg ctgcggaatc ggttggacgc
     2761 actttcgcgc aagctgttcg gcgacggttt agcgcgggat acggggcact cgacttttca
     2821 aaccgcctcc agaacggcca gtggcaacca gccggtctga tcgttcggca ggcgaacttc
     2881 ggcccagtcg ccgcgctgct ggagcacgcg catttccacg ccgggcccca gtggttgctg
     2941 cagtgcggga tcgtagccct caccgcgccc caatcgcagg atggtagggc cgccgaccag
     3001 cacagcacgc gggtgacgcg cgtcctcgcg cacttgccaa gccaccgaag ccgcgaatga
     3061 aagcgccagc cccaccgcca gcccgcccgt gaccacaagc tcgcgccgcg gccagcgcag
     3121 acgcagcgtg agcaagaacc agccggctac ggacagtatc acggcggccc agaaccggcc
     3181 gttgagagcc gtgtcatagt gcaggaaaag aagactgcgg acgagacggg tctgtccgct
     3241 gcgctcgatg gccggttcga cttggcgccg cgcgtattcc agattcgccg cgattttctg
     3301 gtcggcggga tcgagtagct gggcccgggc gtagtgcaga atggccgccc cgagttcatt
     3361 gagccggaag taggcgttgc ccaggttgta ttcgagtgcg gcgtttcgca cgcctgcctc
     3421 gaccaacgca ctgaacccgg ccacggcctg gcgatagagc tcctgcgacc gctccggctg
     3481 actgtgcgcc aactcgaccg cctgatcgaa tgcgccttgg gcttcgcgca acacctgccg
     3541 ctgtcgttcg ggggacattt cctccgcgat ggacgctgag cacgtcagcc ccacgatcgc
     3601 aataatggaa agaatcattc gcctcatagc cgctcgcgct ccagggcgcg cagacattgg
     3661 tcggcgcttt cggccagcgc ggtaggggtg tcggtcccca gtccgccaaa tgagagctgc
     3721 tcgcaacgct cgacgatgct ctgccattgc tcgcgaacgg aggcggacgc gttgcgctgg
     3781 gccaggaaac cagccgcggc cgggccgatg aagcgcgcgg gcggctcgtc cagccgttcc
     3841 gccaggtagc ccgtcagcgc agccgtgatt tcggcggccg cttggcgagc cggcagtttc
     3901 tgggcttgag cgaggcgcgc ccgggcttgg cgcagggctt tctggcggcg atggcggatc
     3961 gatccgccgg ctcgcgcgta gctcaggcac acccaggcaa tcaggtaccc ggccggcggc
     4021 ccgatggttg ccagagccag cgcattcagg ctgacgggcg ccgtgctcgc caggagccgc
     4081 gcttcgcgcg tttcgtttcc gtgcaggccg tcaagggcgt gcaatgtctg attgttggcc
     4141 gccggcgggt ccatccgctc gaccagcgcg gaggtgttca gctccgcggc gggcataacc
     4201 ttcaacggaa tggcctcact cagggccacc acgtacatct cgcggtcagg atcgaaatac
     4261 ggatattcca gccgcggaat ctcggttaga tcgctgcgct ccgcgcggat cacttgtgta
     4321 aatctcttgc gaccgccgac gacctcccct gccaactcct cctgcgggac gcggaacccg
     4381 cccgttagct tggggtcagc ggccagattg ggcggcggga gcgtttccag cggtccgtcg
     4441 ccgcggattt ccatcgtcag tttgatcggg tcgcccacgc ggacggcggt cggctccgcc
     4501 aacacggtca gatcaaatgt gccgaccgct ccggtgaaat ttgcggggcg cccctgggtg
     4561 ggcagcgcta gcacggaaac gtcggaaggc ttcggcgtgg caatcagggt gcgataggcg
     4621 gtcgcctcca gccgcccaaa aatgtcgcgc gtcagccgca cgggataccg catgcccacc
     4681 tgaatgtcgt cgaaattgag caagccggga cggtcgggga cgtaatccgt gatggtccgg
     4741 tacgtgaaat aggtttcttt gccgccgtcg ggccggtcga gcttgcgcgt atcacgctcg
     4801 atgttgttcg tcggaaaggg gccgaagttc acggcaatca ggaagttcat catgtcctga
     4861 ctgctgatgc gatagccctg aacctgcagc ggcttgacgt agatcgtcat gaccaggcgc
     4921 actcgctgtc cgacgtaaat cctttgccgg tcgcaggtta tctcggccca cagcagttcc
     4981 tgggcatcgc tgggatgcac ctttacctgc acgggttccg tctgcaccgt gcgcccgtcc
     5041 accacgagct tgattggcgg aatcgtcagt tcgcccggct cttgcggcgt cacgtgaaag
     5101 gtgtacgtcc gcgaacgctt aaccgtcgtg cggccgttga ctatggtcgt gaacatcgat
     5161 tcgccgccgc cctcaacccg gcgcacgatc gcctgcggaa ggtcgggaaa atcgggctgt
     5221 tcgcagcggc tgaagtcttc gacccggatg ctgacggtga acggctcgtt ggcgtagatc
     5281 tcgtccggtt caatttccag gctgaccgag gctttggcga acccgggaac ggcgcccgcc
     5341 cccagcagga gcaaaatgac gcacacggca ggtcctgtaa ctgtcggttt taccctcaaa
     5401 ccgggaactc catgttcttc gattccggac ggccaagcgg gcccgttccg gtcagaattc
     5461 taaaggcggg cggacctacg gtgaacccgc cgcggcccgg atcgcggcga acgggtcggc
     5521 tgtatctgcg gccgcttgga cgatgtccag aaaggctcgg aaatcgttgt ttacagcttc
     5581 aagagcggcg gcaaaggcgt ccagattgac gtgataacgc tggtgcaggc ggacgtaagc
     5641 gttgttcgtg ggaatacgcc cccagccggc gctgcgctcc ggaagctgaa gtttcggggc
     5701 cacctccgtc gtgaaggcgt cgcgcgccgc ctgaaacacg gcctcgcgcc cgccgatctt
     5761 cgcttcgctt ggcaagtcgc tggcgtaata cgtgcgcaag tcggtgttga gcttcaggac
     5821 ccaatcggtc acgagttcat catcggcgta ctgggcctgc agatccgccg cgatggccgc
     5881 cccctctggg ccgcgctgct ccaggaacag ccgcgcgccc tggcggccga tgaatgtggc
     5941 caggctctcg ttgaagtcgc tctgaccgga gacgtaaatc gtgttgtgcg ccagctcatg
     6001 tatgaccgtt tcgaccaggt ttccgtcggc gcgattcaag aacgttgact ggaccgggtc
     6061 gggcaaccag ccgagcgtgc tgtaggcgtc cactccgtaa atgaacgtgt catagccctg
     6121 tgcctcgagc tgtgcggcgg ccttttccgc atcggctttg gcgaagtagc cgagatagtc
     6181 gatcgtgccg acgatcggca tgcgccattg tttaggcgaa agcgagtcct tgcggcaggc
     6241 cgagaggttg tatgcgatgg gcttaccgtc ggcttcatga tacaggcgat aactgcggcc
     6301 gacgttcaac cccagttcgg acgcggcgaa atcgcgtacc tcaacgacca ggcgcagctt
     6361 gcgctcctgc tcaggggtaa ggcgcccgcc ggccaggacc tcgtcgatgg gctcgctgtt
     6421 gacgagcatc tcgagttggc ccgcggcctg gcggatgatg tagccgaagt cggcgcagcc
     6481 gctgagcacc agcggcagca gcgcgagcag cggggcgcat ggggcgcgaa ggagataaat
     6541 cgcatgtcgt ggcactgaag cgcacctcaa ggtccaaggg ccggtgtcgc tcgccggaag
     6601 ggaagctatc atacccgcgt tatcgagagg ttcctatgac cgaagagaag ccggttgcac
     6661 tgcccacgct cgtgtcgctg ctcatttgcg accaggtgat cgacgacaag ctgaccaaca
     6721 agaagagcgc gatcggcatg ttcaacacgg tgctggtccc gcaagctccg actacgctgg
     6781 cccaggtcat ggttttggcc tcgctgaccg acattaaggg ccgcgtggtg ctgcggctgc
     6841 ggctggtgcg cgacaccgat aacgagatcc tgtttgagac ccacgggccg atcgaatcgc
     6901 ccgatccgct
//
LOCUS       DAOSYS010000192         6882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9371_length_6882_cov_5.273326, whole genome shotgun sequence.
ACCESSION   DAOSYS010000192 DAOSYS010000000
VERSION     DAOSYS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9371_length_6882_cov_5.273326"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PLU99_11575"
     CDS             <1..1704
                     /locus_tag="PLU99_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOO17733.1"
                     /translation="REDPEQRLICEIAFQYTDGYNENILSFANNINTVEGGTHLSGFR
                     SALTRTLNNYARKENLLKPHHPTPTGDDTREGLTAVIAVKVPEPQFEGQTKTKLGNGE
                     VGTFVETVVGEMLGMYLEEHPADAKRIVTKAVQAALAREAARKARETARKSAMSGGGF
                     SRKLVDCSSRDVDSTELYIVEGDSAAGSAKGYRDAKTQAILPIRGKILNVEKARLHKV
                     LSHEEILEIIRALGTGIGAEEFDASKLRYGRIILMTDADVDGSHIRTLLLTFFFRHLR
                     PLIDKGVVYIAQPPLFQISKGKKAEYVLDDGELNALLTRLGRAGAALEIRRPQQEVRT
                     LPPERLGELLRVVEEIEAQARILARRGISLGQFMQRLSASGALPRIRAHADGLEGYFY
                     AEEEFETYRREHLARGLSVVKNELTEVPALQASLARLADFGCDSQDLFLRREEQITGE
                     LSPAIFVLVPPSGPPVELNNLLELPAAVRSVGARGREIKRFKGLGEMNKEELWETTMN
                     PANRVMRRVIVGETADDPEQSELDAAEADRVFSILMGENVEDRKKFIEDNAIRVKNLD
                     I"
     gene            1798..2325
                     /locus_tag="PLU99_11580"
     CDS             1798..2325
                     /locus_tag="PLU99_11580"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOO17734.1"
                     /translation="MDAGEIVLTARRFVVRRRQRPGRDGKLHTYEIVEHPGAAVILPL
                     LDDGRVVLISNYRIALDRELLELPAGTLDPPEAPLECARRELAEETGYQADSLTPLVA
                     FYSTPGICTEQMHVFVARDLRPGPQALGAGEEIRLEPMEYAEALRAVADGRIVDAKTI
                     VALLYYHQYHGTPTR"
     gene            2371..3405
                     /locus_tag="PLU99_11585"
     CDS             2371..3405
                     /locus_tag="PLU99_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOO17735.1"
                     /translation="MNFLGGSIRIGQLFGINLRVHVLFLIWIGYILLTAPAEARLSEG
                     LFWGMLFGIVLLHELGHCFGARMMGGEANDIMMWPLGGLAYAAAPMRPWPQFVTVAAG
                     PAVNVLLCLISGTILATVGGPVEFSINPFSAVRISHLAPVWVEVLAMFYHVNYFLLAF
                     NLLPIYPLDGGQILHSIVWPFVGLQRATIIACYIGLAGAIYLGVRGLQGGGMLLFIAI
                     FGGLTCWQRLRMAQAGMLVEDDRFTVYEPADRPGLFKRLFGRRSRGRRSHSPIEYPEG
                     RPSPNPNPGAWDAKMSEQQRREAEIDRILKKVSEQGVQSLSYVERQTLEQATRERQER
                     EREFQRETRL"
     gene            complement(3440..5353)
                     /locus_tag="PLU99_11590"
     CDS             complement(3440..5353)
                     /locus_tag="PLU99_11590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO17736.1"
                     /translation="MISVTDAKGPFASETPVPQVAPEQPPIGLESPSEAALLFEVSWE
                     VCAQAGGIYTVLRSKAPATVRRWADAYFLVGPYREASARVELEEQPPPEVVRGAIANL
                     EQSGVKAHFGRWLVTGRPYALLLDSSALKQRLGEIKYSLWTDVGISSPDGDFEYDDTL
                     CFGVALIDFLAGVVRHAGRRPVLAHFHEWQAAAALPLLRRRAVPLATVFTTHATLVGR
                     ALASANADLYGSLANINAGAVARAHGFEHRHALEGSAAQSADVFTTVSSITAQEAEHF
                     LGRRADVLLPNGLNVERFAAPHEFQVLHRQNKEVIHQFVMGHFFPSYHFDLDNTLYMF
                     SSGRYEYRNKGLDIFIDALGELNRRMQAENSPVTVVAFIITRAAYRALNVETLNRQAM
                     FHELRDTCQDIEQTMGRRLFHKVATGRLPGIGDLLDEYALVHLKRMMYAMQRQTLPTI
                     VTHDLVDDTHDPVLQHLRSARLWNRPEDRVKVIFHPEFMSSTSPLLAMDYDQFVRGCH
                     LGVFPSYYEPWGYTPLECVVRGIPAITSDLSGFGQYVMENFPDHDTEGIFLARRRDVS
                     FAATVAQVAGWMHSLTRMSRRERIALRNRVESHAEDFDWSRLEQHYASARRLALQLHY
                     PGPDRVPEAQKQT"
     gene            complement(5364..6065)
                     /locus_tag="PLU99_11595"
     CDS             complement(5364..6065)
                     /locus_tag="PLU99_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HOO17737.1"
                     /translation="MKLARLHAWPRTPTAGVQLQHRLAMRVVLQPVTGRTRLMAGADA
                     AFCDNGASALGGVVVWDRLTQTVVEQSTARVACRFPYIPGLLTFRELPALLAAFRRLR
                     TMPEVVFADAHGLAHPRRMGLACMLGLWLNVPTVGCAKSLLCGEHGTLGWKRGSAVPL
                     VHKGEQVGAVLRTQSGVKPVYVSPGHLCDCESACRLTLAATSKYRLPDSIRQAHQLVT
                     RTKVLLVKRPRYSIG"
     gene            6230..>6882
                     /locus_tag="PLU99_11600"
     CDS             6230..>6882
                     /locus_tag="PLU99_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOO17738.1"
                     /translation="MDEVFMVPSQVPVFPHPELVLFPRAVLPLHMYEPRYRQMIADVL
                     ESDRFIATALLKPGWEPHYHTLHAPIHGVVSIGHIVAAEKTEKGDYNVLLRGLIRARV
                     LEEIEHEPYRVVRVRPLKDVQEVPPAALEKSRHTLRETIETEPIGDDEVRAYWLKLLG
                     TQLGLGDVADLIASALPVDAELRQCLLAELDPVARTSLLGDHIRTMASVMRVRRTQNP
                     "
BASE COUNT         1262 a         2111 c         2305 g         1204 t
ORIGIN      
        1 cgcgaggacc cggagcagcg gctgatttgc gagatcgcgt tccagtacac cgacggctat
       61 aacgagaaca tcctctcgtt cgccaacaac atcaacacgg tcgagggcgg aacgcacctg
      121 tcgggtttcc gcagcgcctt gacccgtacc ttgaataact atgctcgcaa ggaaaacctg
      181 ctcaagccgc atcacccgac gcccacgggc gacgatacgc gcgaaggcct gaccgccgtg
      241 attgcggtta aagtgcccga gccgcagttt gaggggcaaa cgaagaccaa gctcggcaac
      301 ggcgaggtcg gcacgttcgt cgagacggtc gtgggcgaga tgctgggcat gtacctggag
      361 gagcacccgg ccgacgccaa gcgcatcgtc accaaggcgg tgcaggctgc cttggcccgc
      421 gaggcggctc gcaaggcccg cgagacggcc cgtaaatcag ccatgtcggg cggcggtttc
      481 tcacgcaaac tcgtggattg ctccagccgc gacgtggatt ccaccgagct gtacatcgtg
      541 gagggggatt cggcggccgg ttcggccaaa ggctaccgcg acgcgaagac gcaggccatt
      601 ctgcccatcc gcggcaagat tttgaacgtg gaaaaggccc gtttgcacaa ggtgcttagc
      661 cacgaggaga ttctcgagat cattcgcgcc ctcgggacgg gcatcggagc ggaggagttc
      721 gacgcctcca agttgcgcta cgggcgcatc atcctgatga ccgacgccga cgtggacggc
      781 tcgcacatcc gcacgctgct gttgacgttc tttttccggc acctgcggcc gctgatcgac
      841 aagggcgtgg tgtacatcgc gcagccgccc ctctttcaaa tctccaaggg caagaaagcg
      901 gagtatgtgc tcgatgacgg cgagctcaat gcgctgctga cccgcctggg acgcgccggc
      961 gcggccctgg aaatccgccg cccgcagcag gaagtccgca cgctgccgcc cgagcggctg
     1021 ggtgagctgc tgcgcgtggt ggaggaaatc gaagcccagg cgcgcatcct ggcgcggcgc
     1081 gggatcagtc tggggcaatt catgcagcgc ctcagcgcga gcggcgcctt gccgcggatc
     1141 cgcgcccacg ccgacggcct ggagggctat ttctatgccg aggaagagtt cgaaacttac
     1201 cgccgcgagc acctggcccg cggcctctcg gtcgtcaaga acgagctgac cgaggtcccc
     1261 gccctgcaag ccagcctggc gcgcctggcc gacttcggct gcgactcaca agatctcttc
     1321 ctgcgccgcg aagagcagat caccggcgag ttgagcccgg ccattttcgt gttggttccg
     1381 cccagcggcc cgccggtcga gctgaacaat ctgctcgagc tgccggccgc cgtgcgctcg
     1441 gtcggggcgc gcggccgcga gatcaagcgc ttcaagggct tgggcgagat gaataaggaa
     1501 gagctgtggg aaaccacgat gaatccggcc aatcgcgtca tgcggcgcgt gatcgtgggc
     1561 gaaacggcgg atgatcccga gcagtccgag ctggatgcgg ccgaagctga ccgcgtgttc
     1621 agcattctga tgggcgagaa cgtggaggat cgcaagaagt tcatcgagga caatgcgatt
     1681 cgcgtaaaga atctggatat ttgagtacag gccaaatctc cggagtgtca cgtcaggccg
     1741 cgaaacggtc agcgtcgggc ggggcgtccg gcgaaacatc caaaccagga gcccacgttg
     1801 gacgcgggtg aaattgtctt gacggctcgc agatttgtcg ttcgccggcg gcagaggccg
     1861 ggacgcgacg gcaagctgca cacgtacgag attgtggagc atccgggcgc ggcggtaatt
     1921 ctaccgcttc tcgacgacgg ccgcgtggtg ctgatctcga actatcggat cgcgctggac
     1981 cgcgagctgc tggagctgcc ggccggcacg ctcgatccgc ccgaagcgcc gctcgagtgc
     2041 gcccgccgcg agttggccga agaaacaggt tatcaggccg acagcttgac cccgctggtt
     2101 gcgttctatt ccacgcccgg tatttgcacc gagcaaatgc acgtttttgt ggcccgcgac
     2161 cttcgccccg gtccgcaggc tctgggagcg ggcgaggaga tccggctgga gccgatggaa
     2221 tacgcggagg ctctgcgggc ggtggccgac ggccgtattg tcgatgccaa gaccatagtg
     2281 gccctgctct actatcatca gtaccacggc acgccgaccc ggtagggcag cgccgcggcg
     2341 ccccgccaac gctgggcaag gactggatga atgaattttc tcggcggttc tattcgaatc
     2401 gggcagctct tcggcatcaa tctgcgcgtc catgtgctgt tcctgatctg gatcgggtac
     2461 atcctgctga ccgctccggc cgaggcgcgg ctttccgagg ggttgttttg gggcatgctg
     2521 ttcggcatcg tgctgctcca cgagctggga cactgctttg gcgcgcgaat gatgggcgga
     2581 gaggcgaacg acatcatgat gtggccgcta ggcggattgg cctacgcggc cgcgcccatg
     2641 cggccatggc cgcagtttgt cacggttgcg gcgggtccgg cggtgaacgt gctgctgtgt
     2701 ctgatcagcg ggacgattct cgccacggtg ggcgggccgg tggagttctc aatcaaccca
     2761 ttcagcgcgg tacgaatcag ccacctcgcc ccggtgtggg tcgaagtgct ggcgatgttc
     2821 tatcatgtga attacttcct gctggcgttc aacctgctgc cgatttatcc gctcgacggc
     2881 gggcagatcc tgcattcgat cgtctggccg tttgtaggcc tgcagcgcgc aaccataatc
     2941 gcgtgctaca ttgggctggc aggcgcgata tacctgggcg tgcgcggctt gcagggcggc
     3001 ggcatgctgc tcttcatcgc gatctttggc gggctcacct gctggcaacg cttgcgtatg
     3061 gcacaggccg gcatgctggt tgaagacgac cggtttacgg tctacgagcc ggcggaccgg
     3121 cccggcttgt tcaagcggct ctttggccgg cgttcgcgag gaaggcgttc acactcgccg
     3181 atcgaatatc ccgaggggcg gccgtcgccg aatccaaatc ccggcgcctg ggatgccaag
     3241 atgagcgagc agcagcggcg tgaggcggag atcgaccgca ttttgaagaa ggtttccgag
     3301 cagggggtgc aaagcctgag ctacgtcgaa cggcagaccc tggagcaggc gacgcgcgaa
     3361 cgccaggagc gcgagcgcga gttccagcgg gagacgaggc tgtagccggg gtcgtcgcca
     3421 ccgcctcgaa gtcggtttct caagtctgtt tctgcgcttc gggaacgcgg tccgggccgg
     3481 ggtagtgcaa ctgcagggcc aggcggcggg ctgaggcgta atgctgttcc agcctgctcc
     3541 agtcgaagtc ctcggcatgg ctttccacgc ggttgcgcag ggcgatccgc tcgcggcgcg
     3601 acatgcgcgt caggctgtgc atccaacccg ccacttgcgc caccgtcgcc gcgaaactca
     3661 cgtcgcgccg gcgggccagg aatatcccct ccgtgtcgtg gtcgggaaag ttctccatga
     3721 cgtactgccc gaagccgctc agatcgctgg taatggccgg gatgccacgt acgacgcact
     3781 ccagcggcgt gtagccccac ggctcgtagt acgacggaaa cacgcccaaa tgacagccgc
     3841 gcacaaattg gtcgtaatcc atcgccagca gcgggctggt gctgctcatg aactcgggat
     3901 ggaagatcac cttgacgcga tcttccggcc ggttccagag gcgcgcgctg cgcaggtgtt
     3961 gcaaaaccgg gtcatgcgtg tcatccacca ggtcgtgcgt gacgatggtg ggcagcgtct
     4021 gccgctgcat cgcgtacatc atccgcttga ggtgtaccag cgcatattcg tccagcaggt
     4081 cgccgatgcc cggcaggcgg ccagtcgcga ccttgtggaa cagccggcgc cccatggtct
     4141 gctcgatatc ctggcacgtg tcgcgcagct cgtgaaacat cgcctggcga ttgagcgttt
     4201 ccacgttgag ggcccggtag gccgcgcgcg tgatgatgaa cgccaccacg gttaccggag
     4261 aattctcggc ctgcatgcgg cgattgagct cgcccagcgc gtcgatgaag atgtccagcc
     4321 ccttgttgcg gtattcgtac cggccggaac tgaacatgta gagcgtgttg tcgaggtcga
     4381 aatggtagct ggggaagaaa tgccccatga cgaactggtg gattacctcc ttgttctgcc
     4441 ggtgcagcac ctggaattcg tgcggggccg cgaagcgttc cacgttcagg ccgttcggca
     4501 ggagcacgtc ggcccggcgg ccaaggaaat gctctgcctc ctgagccgtg atcgacgaca
     4561 cggtggtgaa cacgtccgcc gattgagcgg ccgagccttc gagtgcgtgg cggtgctcga
     4621 acccgtgggc ccgggccacg gcgcccgcgt taatgttcgc caggctgccg tataggtccg
     4681 cattggcgga ggccaaggcg cgtccgacga gcgttgcgtg cgtagtgaag accgtcgcca
     4741 acgggaccgc gcggcggcgc agcagcggca gggcggcggc cgcttgccat tcgtgaaagt
     4801 gtgccagcac cgggcgccgg ccggcatggc gcacgacgcc cgccaggaaa tctatcagcg
     4861 cgacgccgaa acagagcgtg tcgtcatact cgaagtcgcc gtcgggagac gaaataccga
     4921 catccgtcca cagcgagtac ttaatctctc ccaagcgctg cttcagagcg gacgaatcaa
     4981 ggagcagggc atagggccgt ccggtgacga gccagcggcc gaagtgggcc tttacgccgg
     5041 attgctccag gttggcgatc gcgccgcgga cgacctccgg cggcggctgc tcctccaact
     5101 cgacccgcgc agaggcctcg cgatagggtc cgaccagaaa gtatgcgtct gcccagcgcc
     5161 gcaccgtggc cggggccttg ctgcggagaa ccgtatatat cccgcccgcc tgggcacaga
     5221 cctcccacga cacttcaaac agcaacgccg cttcagaggg agattcaagg ccgatcggcg
     5281 gctgctcggg tgcgacctgc ggcacaggcg tctcgcttgc gaagggacct tttgcatcag
     5341 tgacggaaat catttggact cagtcaaccg atactatacc gcggtcgctt gacgagcaga
     5401 accttagtgc gggtcacaag ttgatgtgcc tgccggattg aatctggcag acggtacttc
     5461 gatgttgcag ccaacgtcag ccgacaggcg gattcgcagt cgcacaaatg gccgggactg
     5521 acgtaaacgg gctttacgcc gctctgagtg cggagcacgg cgcccacttg ctcccctttg
     5581 tgcaccaggg gaaccgcact gccgcgcttc caccccagcg tgccgtgttc gccgcacagc
     5641 agcgatttgg cgcagccgac ggtgggcacg ttcagccaca gaccaagcat gcaggctaaa
     5701 cccatcctgc gcgggtgcgc cagaccatga gcatcggcga atacgacctc cggcatcgtc
     5761 cgaagtctgc gaaacgccgc cagcagcgcc ggcagttcac gaaacgtgag cagaccaggg
     5821 atgtagggaa agcggcatgc gacgcgggcc gtcgactgct ccacgaccgt ttgtgtcagg
     5881 cggtcccaca ccacgacccc acccagggca ctcgcaccgt tatcacagaa agcggcgtcc
     5941 gctccggcca tcaagcgcgt ccggccggtc accggctgca gcacgacgcg catcgccagg
     6001 cggtgctgga gctgcacgcc ggcggtgggg gtacggggcc atgcgtggag ccgggcgagc
     6061 ttcatgattt tgattctacg gctgcagaat gttcagggca tggccgattg gctcgttagc
     6121 gacggtggca aacaactcgc ccgtggacga gcaagtgggc gaggttttga ggctcggatt
     6181 cggtcccaac gactgtataa tgtactttac ggccagcaga gcttagcgca tggacgaagt
     6241 tttcatggta cccagccagg tgccggtgtt tccacacccg gaattggtgc tgtttccacg
     6301 cgccgttctg ccgctgcata tgtacgagcc gcgatatcgg cagatgatcg ccgatgtgct
     6361 ggagagcgac cgcttcatag cgacggcgct gctgaaaccg gggtgggagc cgcattacca
     6421 cacgctgcac gcgccgatcc acggggttgt gagcatcggg cacatcgtgg cggccgagaa
     6481 gacggagaag ggggattaca acgtgcttct gcgggggtta atccgggcgc gcgtcctgga
     6541 ggaaatcgag catgaaccct atcgcgtggt tcgtgtgcgg ccgctgaagg acgtgcaaga
     6601 agtgcccccg gcggcgctgg agaaatcgcg gcacacgctg cgcgagacga ttgaaacgga
     6661 accgatcggg gacgacgagg tgcgcgcgta ctggctcaag ctgttgggga cgcagcttgg
     6721 gttgggtgac gtggccgacc tgatcgccag cgcgcttcca gtggacgcgg agttacggca
     6781 atgcttgctg gcggagctcg acccggtggc gcggaccagc ctgctgggcg accatatccg
     6841 cacgatggcc tcggtgatgc gcgtgcggcg cacgcagaac cc
//
LOCUS       DAOSYS010000193         6787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9555_length_6787_cov_5.277035, whole genome shotgun sequence.
ACCESSION   DAOSYS010000193 DAOSYS010000000
VERSION     DAOSYS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_6787_cov_5.277035"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1163
                     /locus_tag="PLU99_11605"
     CDS             354..1163
                     /locus_tag="PLU99_11605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HOO17739.1"
                     /translation="MFDSQQPQSDQPRVWDERALRDPHRQLDKAGRVQAMFNAIAGTY
                     ELINTLATFGRDAVWRRRAVAAADVRAGDVILDVACGTGDMIRAFARLCAPEAPGLKG
                     RVPARIIGVDFAREMLARGDYSRVSVPVELIEADALRMPLADESADVISCAFGVRNFQ
                     DLQAGLAEMYRVARPGARIVILEFAPPRSRFLRWLNERYCETVLPCLGALVARDRVGA
                     YRYLPRSVESFEPVDAMMRRLRDAKFVNVTARAMNFGGVVLYRGEKAAAAD"
     gene            1196..3166
                     /locus_tag="PLU99_11610"
     CDS             1196..3166
                     /locus_tag="PLU99_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOO17740.1"
                     /translation="MKVVPIALLFTVVAGHSAPAAETDYRHSRESTAAIETAVVEYVN
                     VAQLPDEWRTHAERTGYEETGRYDEAVAFCRKLADFSPYAKYMSFGTSGEGRELPLLV
                     LSKDRRFEPDVRGRPAQALVRAADSSMQDFALDSRAELVLAAEQRAAGPLSSKPRVLL
                     QCCIHAGECEGKDASLALARDILVTGVHAALLNGLDLLIIPIFNVDGHERFGPYNRLN
                     QNGPREMGWRTTAVNLNLNRDYMKADAVEMQAWLGLWMAWAPDLLLDSHATDGSDCQY
                     DMFYTASIGPGLDSGISEWTQKIYLPELLAGLGADGHLAIDYCFPRDQRDLTKGFATW
                     SGFAPRLSHCYAALCNRPSILVETHSLKPYPRRVQTTYDTMRHTLECVNRHAGELRAA
                     VCAADARAVETRGGETDGAVPLRWRSSEESQPIVYKALEQTFRPSEITGGEVVVYGKK
                     PVDVETVMCNQVCVDQSVTPPAAYLIPPQWSEAIKRFDLHGLEYFRLAEKRALPVRIY
                     EFQNVKFATRPHEGRFSPKYEVRARDEMREFAAGTVVVPMNQPRAKIAAHLLEPQAPD
                     SFVAWGFFNAIFEQKEYAEAYIMEPLAQRMLDEDAALRQEFVNRLKDADFAGNAGARL
                     NFFYRRSRYWDAAMNVYPVGLLFDASLLSGLR"
     gene            complement(3581..5029)
                     /locus_tag="PLU99_11615"
     CDS             complement(3581..5029)
                     /locus_tag="PLU99_11615"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO17741.1"
                     /translation="MLKPFRTLRLKITLLYVVIFGVLQAALWVAIDSTRASLVYRRFD
                     TVLEERAKVILQAVSTLENSGKLGPAAPTTQSVISRLESPDIFFQLRDAQGNLLAASS
                     NAQGVSLPLDTRRTAGPGFSTLSGQLSEALGLEGERLRQVTLQGGPPGRELYLQVASS
                     MGPLDRELNHIRDLLFIFAFVSLVVAAITSWYMARRSLAPLLAIARQAREISARRLDQ
                     RIPVPSSGDEVAEMIIVLNDMLDRLEAEFAAQRRFIADVTHELKTPLAVLLGETQAMQ
                     RQTGKPADYAAYVATVHDETRRILRLVEAFLILNRLQAGAPAPSLDRTSMEEVVLSAI
                     RKCRAAAAKREVHVRAKFPVDGEYEPIVIGDSDMLVSALQNLIENAIAVSETGGTVDV
                     SVEATETDVVTSVSDSGPGIDEDRLAQLFRLFSEPEKKQVGKPGIGLTIARGVAELHR
                     GRLEGHNRPEGGYEFVMRLPRAAESADQHRDS"
     gene            complement(5058..5741)
                     /locus_tag="PLU99_11620"
     CDS             complement(5058..5741)
                     /locus_tag="PLU99_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOO17742.1"
                     /translation="MSILVIEDNAKLASLVQQGLSEHGHEVEITLSGREGEEMAAQGE
                     YEAIILDLMLPDHDGVQICRELRRRGVSTPILMLTALSSTSDKVTGFEAGADDYLAKP
                     FDLEELLARVHALLRRAQSSEAAVLRFADIEMNRVARTVTRAGQPVTLTSKEFALLEY
                     FMRNPNRVLTRTNLGERVWDMNFEDESNVIEVYISRLRNKIDKGFDKPLIHTVIGTGY
                     VLSAERPTG"
BASE COUNT         1255 a         2077 c         2181 g         1274 t
ORIGIN      
        1 tccggcgtgc gacatcaaca acgccgatct taatggcgac ggcacgatta atgtcttcga
       61 cattgatccg ttcgtgctgt gcctgaccgg cggcgagtgc ggttgagccg agttcgcccg
      121 ccggcggctc gtgtcttcaa gcggcgataa cctcggagcg cggcgttgct tcagcgccgc
      181 gtctcgttca caacgccatg ccgcccccac gccatggccg caagccgcag aatcaagcct
      241 gtcgctcgcg acaagatcga gctgacgcgg gcgttgcgag cgacctgacg gcgctgctcg
      301 cggccggcgt gctgttacaa tccacggttc gaaccggcca gcgggagttc gcgatgttcg
      361 attcgcagca gcctcaaagc gatcagccgc gcgtttggga cgagcgcgcc ctgcgcgatc
      421 cgcaccggca gctcgacaag gccgggcgtg tgcaggcgat gttcaacgcg atcgccggaa
      481 cgtacgagct catcaacacg ctggcgacct tcgggcggga tgcggtctgg cggcggcggg
      541 ccgtggcggc ggcggacgtc cgggccgggg atgtaatcct ggacgtggct tgcggcaccg
      601 gcgacatgat ccgggcgttc gctcgactat gcgcgcccga ggcgccaggc ctcaaggggc
      661 gcgtgccggc gcggattatc ggggtcgatt ttgcgcgcga gatgctggcg cgcggcgatt
      721 attcgcgtgt gagcgtgccg gtggagctga ttgaggctga cgccctgcga atgccgctgg
      781 ccgacgagtc ggcggacgtg atcagttgcg ccttcggcgt gcggaatttt caggacctgc
      841 aggccgggct ggcggagatg tatcgcgtgg cgcggcccgg ggcgcgcatc gtcattctcg
      901 aattcgcgcc gccacgcagc cgttttcttc gctggctgaa tgagcgctac tgcgaaaccg
      961 tgctaccatg cctgggcgcg ctggtagcgc gtgaccgcgt cggggcttac cgctacttgc
     1021 cgcggtcggt tgagagtttt gagccggtcg acgcgatgat gcggcggctg cgcgatgcga
     1081 agttcgttaa cgtcaccgcg cgggcaatga actttggggg cgtcgtgctt tatcgtggcg
     1141 agaaagccgc cgcggccgac tgaggatcgc gttgttcgcc aaacggagtt cgttgatgaa
     1201 ggtcgtgcct attgcactct tgttcaccgt agtcgcaggc cactcggccc cagccgcaga
     1261 aactgattat cgccacagcc gcgaatcgac tgccgccata gagactgccg tcgtggaata
     1321 tgtaaatgtc gcgcaactgc ccgatgaatg gcgcacgcat gcggagcgca cggggtatga
     1381 agaaaccggg cgttatgacg aagcggtcgc cttttgtcgc aagctggcgg atttctcgcc
     1441 gtatgcgaaa tacatgagct tcgggaccag tggcgagggg cgggagctgc cgctgctggt
     1501 tttgtcgaag gaccggcgtt tcgagccaga tgttcggggg cgccccgccc aggcacttgt
     1561 tcgcgcggcg gattcgtcga tgcaagactt cgctcttgat tcgcgtgcgg agctggtgct
     1621 agctgcagag cagcgcgccg ccggtccgct ctccagtaaa ccgcgcgtgc tcttgcagtg
     1681 ctgcattcat gctggcgaat gcgaaggcaa ggatgccagc ctggcgttgg cccgggacat
     1741 tctcgtgacc ggcgtgcacg cagccctgct gaacggcctg gacttgctca tcattcccat
     1801 attcaacgtc gacggccacg agcgtttcgg cccgtacaac cgccttaacc agaatggccc
     1861 gcgcgagatg ggttggcgaa ccaccgccgt caacttgaat ctcaaccgcg actacatgaa
     1921 agccgatgcg gtcgagatgc aggcgtggct gggcttgtgg atggcctggg cgccggacct
     1981 gctcctcgac agccacgcga ccgacggcag cgattgccaa tatgacatgt tctacaccgc
     2041 ctcgatcggg ccgggcctcg actcgggcat ctccgagtgg acgcagaaaa tctacctgcc
     2101 cgaattgctg gcgggtctgg gggcggacgg gcacctggcc atcgactact gctttccgcg
     2161 ggaccagcgg gatttgacca agggcttcgc gacctggtcg ggattcgcgc cgcgcctctc
     2221 gcactgctac gcggcgctgt gcaaccggcc cagcatcctg gtcgaaaccc attcgctcaa
     2281 gccctacccg cggcgcgtgc agacgacgta cgacacgatg cggcacacat tagagtgcgt
     2341 gaaccggcat gccggcgagc tgcgggccgc ggtttgcgct gcggacgccc gggcggtgga
     2401 aacacgcggc ggcgaaaccg acggcgctgt tccgctgcgc tggcgaagtt ctgaagagtc
     2461 gcagccgatc gtttacaaag cgctggagca aacgttccgc ccgagcgaaa taactggcgg
     2521 cgaggtagtc gtttacggca agaagcccgt tgacgtcgaa acagtgatgt gcaaccaggt
     2581 gtgcgtcgac cagagcgtca ccccgccggc cgcgtacctc atcccgccgc agtggagcga
     2641 ggccatcaag cgattcgacc tacacgggct ggagtacttc cggttagcgg aaaagcgcgc
     2701 tttgcccgtg cggatatacg agttccaaaa cgtgaaattc gcaaccaggc cgcacgaggg
     2761 ccgcttcagt cccaagtatg aggtgagagc ccgcgatgag atgcgtgagt ttgcggccgg
     2821 gacggtcgtg gtgccgatga accagccgcg ggcgaaaatc gccgctcacc tactggagcc
     2881 gcaggcgccg gactctttcg tagcgtgggg cttcttcaac gcgatcttcg agcagaagga
     2941 gtatgcggaa gcctacataa tggagccgtt ggcgcagcgc atgctggacg aggacgctgc
     3001 gctgcggcag gagttcgtca accggctcaa agacgcggac ttcgcgggca atgcaggggc
     3061 gcggctaaat tttttctacc ggcgctcgcg gtactgggat gcggcgatga acgtttaccc
     3121 cgttggtctg ttgttcgatg cgagcctgct ttcggggctg cgctagcccg tgggaagctt
     3181 cgccgtcgtc accaggtgtg gcggtctgcg gcgtggccgg gacgcctttg aaatcagcca
     3241 taacggccag cacggctccc gaaatcagca agatcgcgcc gacgcaaatg cccaaccagt
     3301 tggtgtaagc ttgcatgccg tcggcgagcg gcggcggcag tcgccggcca atcagaaggc
     3361 tcgtcccgca gaacgccacc agcaacaacc cgccaaccaa cagcgccgct ccgagcacgt
     3421 aacaagctct gcctgaacgc gctggtcgct gggtcgcaaa ggagtgttgc acgatagaag
     3481 ttccgcgcat gcacctaagg tccaccacac tgatgataca aaatcatcat cgccgcgctt
     3541 cggccgcgcg gccaattaaa aacaatttag gatcggctgc tcaggagtca cggtgctggt
     3601 cggccgattc cgcagcgcgc ggcagccgca ttacaaattc gtagccccct tccgggcgat
     3661 tgtggccctc aagcctgcca cggtgcagct cagccacgcc gcgggcgatg gtcagcccta
     3721 ttcccggctt gcccacctgt tttttctcgg gctcgctgaa caggcggaag agttgcgcca
     3781 gccggtcctc atcgattccg ggcccactat cgctaacgga agttacgacg tctgtttcgg
     3841 tcgcctcaac cgagacgtcg accgttccgc cggtttcaga aaccgcgatc gcgttttcga
     3901 tcaagttctg aagggcggaa acaagcatgt cggaatcgcc aataacgatc ggctcgtatt
     3961 ctccatcaac cggaaatttc gcccgcacgt gcacttcgcg tttggccgcg gccgcacggc
     4021 atttgcgaat ggccgagagg accacctctt ccatagatgt gcgatccaaa gacggcgccg
     4081 gcgcgccggc ctgcaggcgg ttgagaatca ggaaggcctc gaccaggcgc aggatgcggc
     4141 gcgtttcatc atgcacggtc gcaacgtaag ccgcgtagtc agccggtttg ccggtttgcc
     4201 gctgcatggc ctgcgtttcg cccagcagca cggccagcgg cgtcttaagt tcgtgcgtaa
     4261 cgtcggcgat gaagcggcgc tgggcggcga attcggcttc cagccggtcg agcatgtcgt
     4321 tcaagacgat gatcatctcg gctacttcgt cgccgctgga gggcaccggg atgcgctgat
     4381 ccagccgccg ggcgctgatc tcgcgcgcct gccgggcaat ggccagcagc ggcgccaacg
     4441 agcgccgcgc catgtaccag gaggtaatgg cggcgacaac caggctgacg aacgcgaaga
     4501 taaacagcaa atcgcggata tggttcagct cgcggtcgag cgggcccatg ctggacgcaa
     4561 cctgcaaata cagttcgcgg ccgggcggcc cgccttgtag cgtcacctgc cgtagacgct
     4621 ctccttccag gcccagggct tcgctgagtt ggccgcttaa agttgagaac ccgggccccg
     4681 cggtgcggcg cgtatccaaa ggcagcgaga cgccctgtgc gttcgatgac gcggccagga
     4741 gatttccctg cgcgtcacga agttggaaga aaatgtccgg actttcgagc cggcttatta
     4801 cagattgggt cgtgggcgcc gcggggccga gcttccccga attctccagc gtgcttacgg
     4861 cctggagaat gaccttggcc cgttcctcca ggacggtgtc gaaccgccgg tagaccagcg
     4921 aagcccgcgt gctatcaatt gcaacccaga gtgcagcctg caggacgccg aagatcacga
     4981 cgtacagcag cgtaattttg agtcgtaggg tgcgaaacgg tttcagcatt aagtcccggc
     5041 ggcggcgccc ggattaatca accggtaggc cgctcggcgc tcagcacata ccccgttcct
     5101 attactgtgt ggatcaaggg tttatcaaaa cccttgtcga tcttgttccg caggcgcgag
     5161 atgtagactt cgatgacgtt gctctcgtcc tcgaagttca tgtcccacac gcgttcgccc
     5221 aaattggtac gcgtcagtac ccggttgggg ttgcgcatga agtactcgag cagcgcgaac
     5281 tccttggacg tcagcgtcac cggctgcccg gcgcgcgtta ccgtccgcgc cacacgattc
     5341 atctcgatgt ccgcaaaacg cagcacggcc gcttccgagc tctgcgcccg gcgcagcagc
     5401 gcgtgtacgc gcgccagcaa ctcttccaga tcgaagggct tggccaggta atcatccgcc
     5461 cccgcctcaa acccggtcac cttgtccgac gtcgacgaaa gagccgtcag catcaaaatg
     5521 ggtgtagata cgccgcggcg tcgcagctcg cggcaaatct gcactccatc atgatcgggc
     5581 agcatcaggt cgagaatgat ggcctcatat tcgccctgtg cggccatttc ctcaccctcg
     5641 cgcccggaga gcgtgatctc cacttcgtgt ccgtgttccg agagcccctg ctgaaccagg
     5701 gaggccagct tggcattgtc ttctatcacc agaatactca taaactctct tccaagttaa
     5761 ggctgccaat ctcgccagat acgacgtgct cccgccgggc gcttcaatcc cgttcttccg
     5821 tccttcctgc cgacggcgac gccatcttac tgctgaccgt tctgccagta tggcagcggc
     5881 tcgccgcaca agtcaagacc aagtgggggt cgcctcagag cgacttactc cgctggcccg
     5941 tctcacacgg gagtatgtcg cacgcttatg aagtccaagt gaattcgagc tgaacaaatt
     6001 gtaacctatc ggccttcaca cacctgaaca acctgtaagc cgccgtccgg cctcgaaaac
     6061 ccagtttcaa cgctgtgggt cggacatttc cgaggcacac gacccgacag aaatattata
     6121 tggcagtcaa atctggcgac tccaaccgtg gggaagtggg aatgtgggaa tgtgggaagg
     6181 tgggaaggtg cggcaggcgc gagcgtgacc tcccttctgc taatatttgt ccgacacggc
     6241 cggtgcacat ttacgtaagc gtacgcagcg ccatgggcca cggaagttgg gtggcgatgc
     6301 cttcagccgt ctgactgcgg cgtcggtggc tctaccggcg gcttggtggg cggcaccggc
     6361 gggctttgtg gcaccgggtg ggtaggcggc gcaggcggtg ccacgggccg gggcgacggc
     6421 tgcggaggag agatcggcgg cgtggccggg tgcggtggct ccagcggctg gacctggtcg
     6481 gcctgcggcc aagttatgtt gttcgtctgt ccagcgggca tttttggctc cctcattggg
     6541 tagccggatt ctagttcgcc ggacggccgg gggcttgaat ttcggctgag ccgtaaagac
     6601 aaccgccaag acgctaagaa gcgccgagac tgccaagacg aatcagattg cagcggggtt
     6661 ggtgtccgct ggcgggtcgt ccaaaagctg atagccgata gctgagagct gacggcttgt
     6721 cgttagccga gatcttggaa cagtaccgtc gacaggtaac gctcgccgaa gctcggcagg
     6781 accgcga
//
LOCUS       DAOSYS010000194         6745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9625_length_6745_cov_5.399402, whole genome shotgun sequence.
ACCESSION   DAOSYS010000194 DAOSYS010000000
VERSION     DAOSYS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9625_length_6745_cov_5.399402"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="aroE"
                     /locus_tag="PLU99_11625"
     CDS             <1..1081
                     /gene="aroE"
                     /locus_tag="PLU99_11625"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOO17743.1"
                     /translation="HGALDACRVLAQLQKWSQARKLIMLATEPAGLITRVLARKFNAF
                     AIFAALDSARQTAPGQPTLRELVEVYRWNQIGPSTTVLGVVGWPVNHSRSPAIHNAAM
                     RAAGINGVYAPLPVAPTYAAFAAFMDHITQNPHLDVTGLSVTIPHKEHAARWLTEHAF
                     PLSDVAQRVGAVNTLTRTGPANWSGDNTDCAGFLAALALVPESGRERTAAILGAGGAA
                     RAAVCALLLRGYNVTVFNRTEARAAALANELGCSAQPWAKRLHTPVEVIVNCTAIGMA
                     PAENESPLPAAALRSKPPATVIDAVYNPAETRLLREARQHGCHIVSGTEVFLAQAAAQ
                     FEAWHKTAAPLDVMRQIMSGKTVPA"
     gene            complement(1207..2490)
                     /gene="clpX"
                     /locus_tag="PLU99_11630"
     CDS             complement(1207..2490)
                     /gene="clpX"
                     /locus_tag="PLU99_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOO17744.1"
                     /translation="MSGTRGTTGGSRGRRGNMCSFCGKSHRDAAPMVEGANDVFICGS
                     CIELCYNLVREERRRAGRSRAPLKSIPKPREIYEYLNQYVIGQAMAKRTLSVAVHNHY
                     KRLAHAETMDPDDVEIEKSNILLIGPTGSGKTLLARTLARVLNVPFAIGDATTLTEAG
                     YVGEDVENVLLKLLQNADYDLEAAQRGIIYIDEIDKIGKTWNNVSITRDVSGEGVQQA
                     LLKMLEGTISNIPPQGGRKHPEQQYIQMDTTNVLFICGGTFVGLDKIIRKRLGRKTIG
                     FSSLQGQADEQRDLGRILEQVTPDDLIEFGMIPEFVGRLPVTCTLEPLDKSALVQILT
                     EPRNALVKQYQKLFEMDNAVLQFTDEALEEIAAQAMERDTGARALRSVLERFMLDYMF
                     DLADLKPGGTYKVTPQVVRGELDLLAPGRLRKESA"
     gene            complement(2633..2881)
                     /locus_tag="PLU99_11635"
     CDS             complement(2633..2881)
                     /locus_tag="PLU99_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOO17745.1"
                     /translation="MTDVLVVSLLVTVVAVAGIGGACVALKVRRWSRQDDQREPFTLQ
                     ELREMRARHEISEQEFQALRGQLLGRAAARTSDKRLSS"
     gene            complement(2945..3718)
                     /locus_tag="PLU99_11640"
     CDS             complement(2945..3718)
                     /locus_tag="PLU99_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17746.1"
                     /translation="MLARLTLVVTALLLAGVATAQLPASGIPQREPSTLEVLNQTIPD
                     FTMQDGSLYEAIEFLQAVAANVNLEVRWKILEDAGVEPEKQNITLKLKNARLSQVLRD
                     LMQQAGGGELLLAYRAVGKSIVISTQKDLGGEMVSKTYDLRDLLMGTAHFEAPQMDVS
                     QALSQVGSGGSGGGSGSTIFGGSGTSGIKGTAGMTGDEGPEMQKILRIIKTTISPDSW
                     EDNGGKGTINPFNGVIVVYNTPLVHQQIAGFVEEEGLGP"
     gene            complement(3950..5167)
                     /locus_tag="PLU99_11645"
     CDS             complement(3950..5167)
                     /locus_tag="PLU99_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17747.1"
                     /translation="MKRMLTIWAVGMAVMAAAGQPPEQGEDRSEWRIQNHVRRLFRAM
                     GSLSDWDTHSAYITDALEKTYERNGWTSEPDRFAMEMMRQVNQVPPWRPDERFDIAAR
                     LMAERYALDDEQQRIMRDTMIQESNDLFVRHSGRIMQYAVEAIQTRAAGEPFTPEQVA
                     RWARLAEPVFLDSRERINAAAQEFASYLRPEQAEALLNDVEAANRRMDDLYVQSQRWA
                     EGDWDPLDWGLEEDPIQTGAGLVPSLADEGGAAGRPERGGRQPAEPEAVEADRGPVPA
                     TSQPGGEDAWAGYVRRFIAKYKLNAAQQERAWRIYRDVTARANAQRGRYGQHIEAAAK
                     RAAARKDEKSQSGVTELQKRLDSQLNQLYMQLKARLDRLPTRQQRAEAEPTELEPSRS
                     VPAPVDPMPHDGP"
     gene            5320..6255
                     /locus_tag="PLU99_11650"
     CDS             5320..6255
                     /locus_tag="PLU99_11650"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOO17748.1"
                     /translation="MPHIKALILRAAGINCDLEAQHAWELAGAQAERVHVRRLIERPQ
                     ILAEYQILMIPGGFSYGDDIAAGRILAAQLKRHLIEPIRDFVASGRLVLGVCNGFQVL
                     VNAGLLPFPDDGVPAGAACERAAAFEAASTHRAYSISDHSAPADDLARLSGLRTCTIS
                     YNEPPGFQDRWVNLRATTRFCAFLDPGREYEMPIAHGEGRVLFADEQTLQRVLSGGLN
                     ALTYVAPPPHAGVTPGAPANPNGSVADIAGLCDPTGRILGLMPHPERFVHWTQHPCWT
                     ADRERLADRCGAEGDGLALFRRAVAYLALSAGKNR"
     gene            6338..>6745
                     /locus_tag="PLU99_11655"
     CDS             6338..>6745
                     /locus_tag="PLU99_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO17749.1"
                     /translation="MSNADDTRRAIAESLKKVIDPATGRDAVASGFARIAYCEGVAKI
                     TYELQPQAVGQDRQAALREQTEAVVGSVPGVQRVNVEFRIVERRATDVIPGVKHVIAV
                     GAGKGGVGKSTVSVILAVGLQRRGLKVGLLDADV"
BASE COUNT         1182 a         2417 c         1880 g         1266 t
ORIGIN      
        1 gcacggcgcg ctggatgcat gtcgcgtgct ggcacaactt caaaagtggt cgcaggctcg
       61 aaagctgatc atgctggcaa ctgagccggc cgggctgatt acccgtgttc tggcccgcaa
      121 attcaacgcc ttcgctatct tcgccgccct cgattcggcc cgccaaacgg cgcccggcca
      181 gcccacgctg cgtgaactgg tcgaagtcta ccgttggaac cagatcggcc cgtcgacaac
      241 ggtactcggc gtagtcggct ggccggtgaa ccattctcgc agcccagcca ttcataacgc
      301 cgcgatgcgc gcggcgggga tcaacggtgt gtacgcgccc ctgcccgtgg cgcctaccta
      361 tgccgccttt gccgcgttca tggaccacat aacgcaaaac ccgcacctgg acgtaaccgg
      421 cctgagcgtc acgatcccgc acaaggaaca cgccgcgcgt tggctgacgg agcacgcttt
      481 tccgttgagc gacgttgcgc agcgcgtggg cgcggtgaac acgctcacac gtacgggacc
      541 ggccaactgg agtggtgaca acaccgattg cgcgggcttt ctggcagccc tcgccctggt
      601 ccccgaaagc ggccgggaaa gaaccgcggc gatcttgggg gccggtgggg cggcccgggc
      661 cgccgtctgc gccctgctct tgcgcggcta caacgtcacc gtcttcaacc gcaccgaggc
      721 ccgtgccgcc gcactggcaa atgagctggg ctgcagcgca cagccctggg caaaacgcct
      781 acacactcct gtagaagtga tcgtgaactg caccgccatc ggcatggcgc cggccgagaa
      841 cgaatcgccg ctgccggccg ccgccttgcg ttccaagccg cccgcgactg tcatcgacgc
      901 cgtctataac cccgcggaaa cgcgcctttt gcgcgaagcg cgccagcacg gctgccacat
      961 tgtcagcggc acggaagtat ttctcgccca agcggccgca caatttgaag cctggcacaa
     1021 aacggccgcc ccgctggacg tcatgcgcca gataatgagc gggaagactg tgcccgcgta
     1081 aattgtggac agtgcaacgg aacagtctgg gatgagctaa ccaccgtgcg cgtgtaacgt
     1141 aaccagtgtg tgcgccggag actgcgcccg gacaaggcgc agccccaatc accccccgcc
     1201 cacacttcag gccgactctt tgcgcagccg ccctggcgcc agcaaatcca actcgccgcg
     1261 gaccacctgc ggcgtcactt tgtaggtccc gcccggtttg aggtcggcca ggtcgaacat
     1321 gtagtccagc atgaagcgtt cgaggaccga gcgcaacgct cgcgcgcccg tatcgcgctc
     1381 catagcctgc gccgcgattt cctccagcgc ctcgtccgta aactgcagga cggcattgtc
     1441 catttcgaag agcttctggt attgcttgac cagcgcgttg cgcggctcag tcagaatttg
     1501 caccagcgcg ctcttgtcca gcggctccaa tgtacacgtg accggcaggc gccctacgaa
     1561 ttcgggaatc ataccgaact cgatcaggtc atccggcgtg acttgctcca ggatgcgccc
     1621 caggtcacgc tgttcgtccg cctgcccctg cagactcgaa aaaccgatcg tcttgcggcc
     1681 gagccgcttg cggataattt tgtccaggcc gacgaacgtg ccgccgcaaa tgaacagcac
     1741 gttggtcgta tccatctgga tgtactgctg ctcggggtgt ttccgcccgc cctgcggcgg
     1801 gatgttgctg atagtgcctt ccagcatttt cagcagcgct tgctggacgc cctcgcccga
     1861 aacgtcgcgc gtgatcgaaa cgttgttcca cgtcttgccg atcttgtcga tctcgtcgat
     1921 gtagatgatg ccgcgctgcg ccgcttccag atcgtaatcc gcgttctgca gcagcttcag
     1981 cagcacgttc tcgacgtctt cgccgacgta cccggcttcg gtgagcgtcg tggcgtcgcc
     2041 gatcgcaaac ggcacgttca gcacgcgcgc cagcgtgcgc gccaagagcg tcttgccgga
     2101 acccgtcgga ccgatcagca gaatattgct cttctcgatc tcaacgtcat ccgggtccat
     2161 cgtctcggcg tgcgccagcc gcttgtagtg gttgtgcacg gccacggaca gcgtgcgctt
     2221 ggccatagcc tgcccaatca catactggtt caagtactcg taaatctcgc gcggtttggg
     2281 aatcgacttc agcggggcgc gcgagcggcc cgcccggcga cgctcttcgc gaaccaggtt
     2341 gtagcacagc tcaatgcacg acccgcagat gaaaacgtcg ttcgcgcctt ccaccatcgg
     2401 cgcggcgtcg cggtggctct tgccgcaaaa gctgcacatg tttccgcgcc gcccacgcga
     2461 accaccagta gtacccctgg taccgctcat tgatcatcct atctgtgcaa aaccgggccc
     2521 tttcccggcc tgcaccttca aggtacccgg aacatgagtt tattttaacc gctgcccccg
     2581 cctgttcaaa tgaatcgcgg ctctccgcga tccgcgtacg cccgaaaatg tcctagctgc
     2641 taagccgctt atcgctggtg cgcgctgccg cacggccaag caactgaccc cgcaacgcct
     2701 ggaattcctg ctcactgatc tcgtgacggg cccgcatttc ccgcaactcc tgaagcgtaa
     2761 acggctcccg ctgatcatcc tggcgggacc accgccgcac tttcagagcc acgcacgccc
     2821 caccgatccc cgccactgcc accaccgtga ccagtagcga tactaccagt acatccgtca
     2881 tacatttgcc ggcaagtcca ggtacaacgc gctggccgaa cctaactctt gagcaggtcg
     2941 cgacttacgg ccccagacct tcctcttcca cgaacccagc gatctgttga tgaactagcg
     3001 gcgtgttata caccacgatg acgccattga acgggttgat cgttcccttg ccgccgttgt
     3061 cctcccagga gtcaggggat atcgtcgtct taataatcct caatatcttc tgcatctccg
     3121 gcccttcatc gccggtcatt cctgcagtac ccttaatacc actcgtcccg ctgccgccaa
     3181 agatggtgga cccgctacca ccaccgctac cgccgctgcc gacctggctc agggcctgac
     3241 taacgtccat ctgcggcgcc tcaaaatgcg ccgtccccat cagcaggtcc cgcaaatcgt
     3301 acgttttcga aaccatctcc ccacccaggt ccttctgggt cgagatcacg atgctctttc
     3361 ccaccgcacg gtaggccagc agcaattcgc cgccccccgc ctgctgcatc aggtcccgca
     3421 acacttgcga cagccgcgcg ttcttcagct tcagcgtgat attctgcttc tccggctcaa
     3481 cgccggcatc ctcgagaatc ttccagcgca cttcaaggtt gacattcgcc gccaccgcct
     3541 gcaggaattc gatcgcttcg tacaggctgc cgtcctgcat cgtaaagtcc ggaatcgtct
     3601 gattgagcac ctccaacgtg ctcggttctc gttgcggaat gccggaagcc ggcaactgcg
     3661 ccgttgccac gccagctagc agcaacgccg ttacaaccag tgtcagccga gccaacatac
     3721 gaagctctcc tttcctgcga cagcctagcg cgcagcctaa aacctgccgc catcgaccac
     3781 tgccacattc taccgctatt cgcatatgtc cggcctgtgg aatccgtcag attttgcgca
     3841 ccatcctaaa atcgccgccc aggggccgga cattaccgct aataagcgca actccattag
     3901 tgacaaacag ttacgaaact ttccgccatc ctgctcgcag acgcctcacc tacggtccgt
     3961 catgcggcat tgggtccacg ggcgccggaa cgctccggga cggctccaac tcggtcggct
     4021 ccgcttccgc ccgctgttgg cgggtcggca gcctgtccaa acgcgccttt agctgcatat
     4081 atagctgatt aagctggctg tccagccgtt tttgcagttc cgtcacgccg ctttgcgatt
     4141 tctcgtcttt ccgcgccgcc gcccgcttgg cagccgcctc aatgtgctgc ccgtagcgcc
     4201 cgcgttgggc gttcgcgcgg gccgtcacgt cgcggtaaat gcgccaggca cgctcctgct
     4261 gcgccgcgtt cagcttgtac ttcgcgatga accgcctcac ataccccgcc cacgcatctt
     4321 caccgcccgg ctgtgacgtc gccggaactg ggccgcgatc agcctcgacc gcttccggtt
     4381 ccgccggctg ccggcctccc cgctccggtc gtcctgccgc gccgccctcg tccgccaggc
     4441 tcggcaccag ccccgcgccc gtctgaatcg ggtcttcttc cagcccccaa tccagcggat
     4501 cccagtcccc ctcggcccac cgctgactct gcacgtacag gtcgtccatg cgccggttcg
     4561 ccgcctccac atcgttcagc agagcctcgg cttgctccgg gcgcagatag cttgcaaatt
     4621 cctgcgccgc cgcgttgatc cgctcgcggg aatccaggaa aaccggctca gccaaccgcg
     4681 cccaccgcgc cacctgctcg ggcgtgaacg gctcgcccgc cgcccgcgtc tgaatcgcct
     4741 cgaccgcgta ctgcatgatc cgccccgaat gccgcacgaa cagatcgttc gattcctgga
     4801 tcatcgtgtc gcgcatgatt cgctgctgct cgtcatccag cgcataacgc tctgccatca
     4861 gccgcgccgc aatgtcgaac cgctcgtccg gccgccaggg cggaacctgg ttgacctggc
     4921 gcatcatctc catcgcgaag cgatcgggct ccgatgtcca cccgttccgc tcgtacgtct
     4981 tttccagggc atcagtgatg tacgcgctgt gtgtgtccca gtcgctcagg ctccccattg
     5041 cccggaacag ccgccttacg tggttctgga tgcgccattc cgagcgatcc tccccctgtt
     5101 ccggcggctg cccggcggcg gccatcaccg ccattcccac cgcccagatc gtcaacatcc
     5161 gcttcataca cgctcccggg cagacagaaa ccgcggcccg tagtacgatt gttgccgctg
     5221 gcgtactgcg attcaatggg cgctttccgc cgctagtatc gtcttcggat cgcgctcatc
     5281 tgatatcggg ccgagctgta aacgtagcgg aggttcactg tgccgcacat aaaagccttg
     5341 atcctgcgtg ccgcgggcat caactgtgat cttgaggccc aacacgcgtg ggagctggcc
     5401 ggcgcccagg cagaacgggt gcacgttcgc cgcctcatcg aacgccccca aatactcgcc
     5461 gaataccaga tcctgatgat cccgggcggc ttcagctacg gcgacgacat cgccgccggc
     5521 cgcatcctgg ccgctcagct caagcgccat ctgatcgaac caatacgcga tttcgtggcc
     5581 tccggtcgcc tggttctggg cgtgtgcaac ggcttccagg tgctcgtaaa cgccggcctc
     5641 ctgccctttc cagacgacgg cgtaccggcg ggcgccgcgt gcgagcgcgc cgcggcgttt
     5701 gaagcggcct cgacgcaccg cgcgtattca atctccgacc actcggcccc tgccgacgac
     5761 ctcgcccggc tgagtggcct gcggacctgc acaatttcct acaacgagcc gcccggtttc
     5821 caggaccgct gggtgaacct gcgggccacg acgcgcttct gcgccttcct cgatccaggc
     5881 cgcgaatacg aaatgccgat cgcccatggc gaaggccggg tcctcttcgc cgacgagcaa
     5941 accctgcagc gcgtcctctc cggcggcctg aacgccctca cctatgtcgc cccgcccccg
     6001 catgcaggcg tgaccccagg cgcgccggcc aacccgaacg gcagcgtcgc tgacatcgcc
     6061 ggcctatgcg acccgaccgg ccggattctg ggtctgatgc ctcatccgga gcgcttcgtc
     6121 cactggacgc agcacccctg ctggaccgcg gaccgcgagc gactggccga ccgctgcggc
     6181 gcggagggcg atggtttggc tctgtttcgg cgagcggtgg cgtatctcgc gctcagcgcc
     6241 ggaaagaacc gctgaaaccc gcggcgcaga cgccccctca ctgcttcctc gacatcgccg
     6301 catgccatac ctatgctcat tgaatcagga gtcataaatg agcaatgccg acgacacccg
     6361 ccgtgcaata gccgaaagcc tcaaaaaagt tatcgacccg gcgaccgggc gcgacgccgt
     6421 cgccagcggt ttcgcgcgca tcgcctactg cgaaggcgtg gccaagatca cttacgagct
     6481 gcagccgcag gccgtcggcc aggaccgcca ggccgcgctt cgtgagcaaa ccgaggcggt
     6541 ggttgggagc gtgccgggcg ttcagcgcgt gaacgtggaa tttcgcatcg tcgagcgccg
     6601 cgccaccgac gtcataccgg gagtaaagca cgtaatcgcc gtcggggccg gcaaaggcgg
     6661 cgtcggcaag agcacggttt cggtgattct cgcggtcggc ctccagcgcc gcggcctgaa
     6721 ggtgggattg ctcgatgccg acgtt
//
LOCUS       DAOSYS010000195         6706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9697_length_6706_cov_5.352278, whole genome shotgun sequence.
ACCESSION   DAOSYS010000195 DAOSYS010000000
VERSION     DAOSYS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9697_length_6706_cov_5.352278"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..1356
                     /locus_tag="PLU99_11660"
     CDS             463..1356
                     /locus_tag="PLU99_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17750.1"
                     /translation="MHQGIAFAQRTGRPLLFYIPPSRDLQGSVFGVNVSDEFGTFDSN
                     IDDAQGIAFRDPIVRAFAQDRFVPVRLQRTNHTLPMLASAGLPTTYGMFLAAFTPRGD
                     FIGWVSPSDAADPRILARRLSELYRDYRLDVFRTDLRPILENGAATPAELNAALRTIS
                     EFMILDADQEVISLLDRRNLDRSVRKQALDTLAVLSTPEAVNTLVSLAGRDRMAANAL
                     NNVTLPAAAYMLSIVDVDDPRQVTAVYDAAAKITGIQNPKYSAFWRQADRSARERELA
                     RVQAVLKPQAQLWHEAIGVLR"
     gene            complement(1399..1824)
                     /locus_tag="PLU99_11665"
     CDS             complement(1399..1824)
                     /locus_tag="PLU99_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HOO17751.1"
                     /translation="MPRHSVIARPMVRGRVSGPAFVCRQAFSFLGDVDMDSGVIIARG
                     HEHEGETIAGKVMIYPETKGSSGGCVVLTVLAKQGKQPAAIVLSKPADPNIVEGAIVT
                     GVTLVCEPAEDLIALVPHGAIVTVDGTTGEVSWERNESA"
     gene            complement(2058..3065)
                     /locus_tag="PLU99_11670"
     CDS             complement(2058..3065)
                     /locus_tag="PLU99_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011405364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOO17752.1"
                     /translation="MNNQAKPAKKQGSVRGPGGPLVIASENGVNACKRAMELLAAGGD
                     PVDAVVAGINLVEDDPEEHTVGYGGLPNEEGVVELDACVMHGPTYKAGAVAALQNIRH
                     ASSVARAVMQHSDHVLLVGAGALQFAKVHGFKEEDLLTEEARRIWLRWKEKLSGHDGR
                     VPPRDAASAIGSRQPEDPDFHYGTITCMALTAGGDLGGCTSTSGLSFKMPGRVGDSSI
                     IGAGLYVDNAVGACGSTGRGEANVLNCSSFLVVELMRRGATPEEACRQALQSVAQRTE
                     RRLRDAEGRPAYSLKLYAIRKDGLVGGASLRGPGRMVVQSRDESRLVDMPALFPARSK
                     E"
     gene            3237..3602
                     /locus_tag="PLU99_11675"
     CDS             3237..3602
                     /locus_tag="PLU99_11675"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOO17753.1"
                     /translation="MQAATELLEKARELGEALARHERVRAYLAAQEAVRQDQQAQTLL
                     RDYARHVDHLRELEASRRPIEVADKHKAADLEAHIAGNARLKELMRMQADYVELINQV
                     NQAIAAPLNPPPQPPGGPA"
     gene            3599..5593
                     /locus_tag="PLU99_11680"
     CDS             3599..5593
                     /locus_tag="PLU99_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17754.1"
                     /translation="MIGRGAAVEPALGAPAGPAQPVPHLRAGVAAWLLALLGWTALLS
                     FYDLDGGAGFEPTDCWVAQTAREMRHAGDWLTPRFSGEVRMQKSPGPYWAVMLTSIVR
                     GTEVDEVSARIPNAIAAIVLVMTVFWLTLHVAGQRAAIFAGFATASSALILLWSHRAA
                     SDLGLAALCTLSLAALWIGSEIESPGPKRVALWMLGYFAAGLGMLYKMPMPLAAVGLP
                     VLAYLLVRNRWRILASPWHLLGLLLFLLPWLPWALAVVHSQGMALAKWRVEFLDRYTG
                     DLPNVEGQDSWAYLLFYLVPVAVYCLPFSLSIPAAVGRAFRRQPGVRRDGTLFMALWF
                     FSLLVFFTASTGKELRYFLPALPPIFVLLGIELAALFDPQRQRSEVLVRLAVAAVFIL
                     LPVALIAGGIGLHRWWELRGRLELAELYQWRDVLWAYLGMALIMALGFGTAAWLYLRR
                     RGNAAFGMIVATMWLMWLWAWPQFMPKLMSQRPFQAFAAELRERVPHALRANMYDIAT
                     HEPRITWYGDVRFPRVIDQLALLAEQGGRRDRDYEARRIGEEVITRLSGTQPLLLVAR
                     LEDYLKLLAVAPQELAAAGRTMPAHHLWLQTPYGDFKRHFVLFGNVPPPWPEPELKIP
                     DGLPDQARQKIMSILAAAQPASAPAASRPSGPQPLPGGSD"
     gene            5593..6615
                     /locus_tag="PLU99_11685"
     CDS             5593..6615
                     /locus_tag="PLU99_11685"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOO17755.1"
                     /translation="MWRAITQRLLEWTEAPTLLIALRYATEYAALRLWSLVIGCFPIE
                     TNLATGRLMGRIWWLAKRSHRERALENLRHSLGDRYTEPELRRIARRSFEHFAQLYLV
                     ELWLTPRIINKWSWARYVELHQLGPALRELLSERGVIMVTAHFGNFELLGFTICRLGI
                     PLVAVMRPLDNPLVNRHLVRARQAGGLSLLYKKGATQSAESILASGGTLCFIGDQDAG
                     RKGIFVDFFGRPASTYKSIGLLAMQQRVPIIVGAATRVGRGFRYRIAVQRIIQPEEWD
                     QQADPLRWVTQTFSHAMEGTIRQAPEQYLWVHRRWKHQPAPRGAQRHHAADSASARTG
                     SAPART"
BASE COUNT         1113 a         2227 c         2070 g         1296 t
ORIGIN      
        1 gcatgcccta agcgcatggg tgccacgccc aagctgtaag cgcgggcatg cccttgttaa
       61 cgacctcacg cccgctgtcg gcatgcccta agcgcatggg tgccatgccc aagctgtaag
      121 cgcgggcatg cccttattaa cgacctcacg cccgctgtcg gcatgcccta agcgcatggg
      181 tgccacgccc aagctgtaag cgcgggcatg cccttattaa cgacctcacg actggtgtcg
      241 gcatgcccgc gccgccacgc ggcttgggca tggcacccac caccgcctac aatcacgagt
      301 cacgctcaga ggataacgcc tatgcatgcc acgccccttt tcgtgcttgc actgttccag
      361 taccccttcc ctccgatgca ggtcgccccg tccccaccgt tttcggcgac ccagctatac
      421 ccctctgtgt tgtcacaaaa tccaatacag tggattgcca acatgcacca gggcattgct
      481 ttcgcgcagc gcaccggccg gccactcctc ttctatatcc ccccttcgcg tgatctgcag
      541 ggaagtgtat tcggtgtaaa cgtctcagac gaattcggaa cgttcgacag taacatagat
      601 gacgctcaag gcatcgcctt ccgcgatccc atagtgcggg cttttgccca ggaccgcttc
      661 gtgcccgtgc gcctgcaacg cacaaaccac acgctcccaa tgctcgcatc ggccggcttg
      721 ccaaccacat atggcatgtt tctggccgct ttcacgcccc gcggcgactt tatcggctgg
      781 gtttcgcctt cggacgcggc cgacccgcgc atcctcgccc gacgcctttc agagctttac
      841 cgcgactacc gcctcgatgt tttccgcaca gacctgcggc caatactgga gaatggagcc
      901 gcgacaccgg cggagttgaa cgccgcgctc agaacgatca gcgagttcat gatcctggac
      961 gccgaccagg aagtgatcag cctgctggat cgccgcaacc tcgaccgcag cgtgcgaaaa
     1021 caggccctcg acaccctggc agtcctgtcc acgccggagg ccgttaacac gctggtcagc
     1081 ctggccggcc gcgatcggat ggcggccaac gccctgaaca acgtcaccct cccggccgcc
     1141 gcctacatgc tctctatcgt ggacgtcgac gacccgcggc aggttacggc cgtctacgac
     1201 gcggccgcaa agatcaccgg cattcagaat cccaagtaca gcgcgttttg gcggcaagcc
     1261 gaccgctccg cccgcgagcg cgaactcgcc cgcgttcagg ccgttctgaa accgcaggcc
     1321 cagctttggc atgaggcgat cggcgttctg cgatagtccg gcttgaaggt ggctcagacc
     1381 actgttacgg gaaacccgtc acgcgctttc gttgcgctcc cacgacactt cgcccgtcgt
     1441 gccgtctacg gtcactatcg ccccgtgcgg gacgagtgcg atcaggtcct cagccggttc
     1501 gcacaccagc gtcacgcccg tgacgatggc cccctcgacg atgttcgggt ctgccggctt
     1561 tgagagcacg atggcggccg gttgcttgcc ctgcttcgcc agcacggtca gcacgacgca
     1621 tccgccggag gagcctttgg tttccggata aatcatgacc ttcccggcga tggtttcgcc
     1681 ctcatgttcg tgcccgcgcg cgataattac gccgctgtcc atgtccacgt cgcccagaaa
     1741 ggaaaacgct tgacggcaca caaacgccgg gccactgacg cggccgcgaa ccatggggcg
     1801 agcgatgacg ctgtgtcggg gcacgttcgg ctcctactgc aagaactggc gcagaatact
     1861 acagtccagc cgcccgaacc acaggtgcgc gccacgggtg cgcgccgtgg gtgccatgcc
     1921 caaggtgtaa gcgcgggcat gccctcgtta acggcatcag gtgcggggcg actgtaaaca
     1981 cgtaaccaag tgcgaggcca attgcagacg gcatgcccgc gccccgctgt cgccgggctt
     2041 gggcatggca cccgagctta ttccttcgaa cgggccggaa aaagcgcagg catgtcgacc
     2101 aaacggctct catcgcggct ctggacgacc atgcgtccgg gaccgcgcaa cgacgctccg
     2161 cccacgagcc catccttgcg gatggcgtac agtttgaggc tgtacgccgg ccgcccctcc
     2221 gcgtccctta ggcgccgctc ggtgcgctgt gcgacgctct gcagcgcctg ccggcaggct
     2281 tcctccggcg tggccccgcg cctcatcagc tcaaccacga gaaaactcga gcagttcaac
     2341 acgttggctt cgccacgccc ggttgagccg caggccccga ccgcgttgtc cacatacagg
     2401 ccggccccga tgatcgaaga gtcgccgacg cgccccggca tcttgaacga caggccggac
     2461 gtactcgtgc agccgccgag gtcgcccccg gccgtcaaag ccatgcaggt aatcgtgcca
     2521 taatggaagt caggatcttc cggctgccgg ctgccgatgg cggaagcggc gtcccgcggc
     2581 gggacccgac cgtcgtggcc ggaaagcttt tccttccacc gcagccagat gcgccgagct
     2641 tcctcggtga gcaaatcttc ctctttgaag ccatgcacct tggcgaactg cagcgcccct
     2701 gctcccacca gcaatacgtg atcgctgtgt tgcataaccg cccgcgccac gcttgaggca
     2761 tggcggatgt tctgtagggc cgctaccgct ccggccttgt acgtcggtcc gtgcatcacg
     2821 caggcatcga gctcaaccac gccctcctcg ttcggcaagc cgccgtaacc caccgtgtgt
     2881 tcttcggggt cgtcctcgac caggttgatg ccggccacga ccgcgtcgac cggatcgccg
     2941 ccggccgcca gcagctccat ggcgcgcttg cacgcgttga ccccgttctc gctcgcaatg
     3001 accagcggcc cgccgggacc gcgcactgaa ccctgctttt tcgccggctt agcttggttg
     3061 ttcataaacg aatcccgctg cggccgcggc cgcgcccatc aacttccgcc gggtagtgga
     3121 cacaggtaac tcctttaccg cccggcttct ccaagcgcga gtgccgacga tatgataccg
     3181 gctgtgtgca ggcggcgata ctgcacggct aacggtatag atcggcaggt gcgaagatgc
     3241 aggcggcgac agaattgctt gagaaggcgc gtgaacttgg cgaggcgctt gcccggcacg
     3301 agcgggtgcg ggcttacttg gcggcgcagg aggccgtgcg gcaagaccag caggcgcaga
     3361 ccttgctgcg cgattacgct cggcatgtgg accatctgcg cgagctggag gcgtcccgcc
     3421 ggcccattga agtcgccgat aagcacaagg ccgccgacct cgaggcgcac atcgcaggca
     3481 acgcccggtt gaaagagctg atgcggatgc aagccgacta cgtcgagctc ataaatcagg
     3541 ttaatcaggc aatcgccgcc cccctcaatc ccccgccgca gccgcccgga gggccggcgt
     3601 gataggacgc ggggcggccg tcgaaccggc cctgggcgcg ccagccgggc ccgctcagcc
     3661 ggttccgcac ttgcgagcgg gcgttgcggc gtggctgctg gcgctattgg gctggacggc
     3721 gctgttgagt ttctacgacc tcgacggcgg cgcgggtttt gaacccactg attgctgggt
     3781 ggcgcaaacg gcgcgcgaaa tgcgccatgc aggcgattgg ctgacgccac gcttttccgg
     3841 cgaagtgcgc atgcagaagt cgccggggcc atattgggcg gtcatgctca ccagtattgt
     3901 gcgcggcacc gaagtggacg aagtcagcgc ccgcattccg aatgcaatcg ccgccattgt
     3961 tctggtaatg accgtcttct ggctcacgct ccacgttgcg ggccaacggg cggcgatttt
     4021 cgccggcttt gccacggctt ccagtgcgtt gattcttttg tggtcgcacc gcgccgcctc
     4081 tgacctgggc ctggccgcac tgtgtacgct ctcgctggcg gctctttgga tcggctcgga
     4141 aatcgaatcg ccgggtccca agcgcgtggc attgtggatg ctcggctatt tcgcggccgg
     4201 tctgggcatg ttgtacaaaa tgcccatgcc gctggcggcc gtcgggctgc ctgtgttggc
     4261 gtatctgctg gtgcggaacc gctggcgtat tctggcaagc ccctggcact tgctgggact
     4321 gctgctgttc ctcttgcctt ggctgccctg ggcgcttgcg gtcgtgcatt cccagggcat
     4381 ggctttggcc aaatggcggg tggagttcct cgaccgctac accggcgatc tgcccaacgt
     4441 cgaagggcag gacagttggg catacctgct gttttacctc gtgccggtgg ccgtctattg
     4501 tctgccgttc agcctgtcaa taccggccgc cgtgggcagg gccttccgcc ggcagccggg
     4561 tgtcagacgc gacggcacgc tctttatggc gctgtggttc ttcagtttgc tggtgttctt
     4621 caccgcctcg accggcaaag aactgcgcta ttttcttcca gcgctacccc caatatttgt
     4681 cctgctgggg attgaactgg ccgcactctt tgacccgcag cgccaacgct cggaggtgct
     4741 ggtgcggttg gccgtcgcgg ccgtcttcat cctcttgccg gtcgccctga tcgcgggagg
     4801 tatcggcctg catcgctggt gggagctgcg cgggcggctc gaactggcgg aactatatca
     4861 atggcgcgac gtactgtggg cgtatctggg catggcgttg atcatggcct tgggcttcgg
     4921 cacggcggcc tggctttatc tgcggcggcg cggaaatgcg gccttcggga tgatcgtcgc
     4981 gaccatgtgg ctgatgtggc tgtgggcctg gccgcagttc atgcccaagc tgatgtcgca
     5041 acgtcccttc caggccttcg ccgccgagtt gcgcgagcgc gtgcctcacg cgctgcgtgc
     5101 gaacatgtat gacatcgcca cgcacgagcc gcgcataacc tggtatggcg acgtgcgctt
     5161 cccgcgcgtc attgatcaac ttgcgttgct cgcggaacaa ggcggacgtc gtgatcggga
     5221 ttatgaagcc cgccgcatcg gcgaggaagt catcacgcgg ctctcgggca cccagccact
     5281 cttgctcgtg gctcgtctcg aagactattt gaaactgctg gccgtagcgc cgcaggagtt
     5341 ggcggcggcc gggcgcacca tgcccgccca tcacctctgg ctccaaacgc cctacggaga
     5401 cttcaagcgg cactttgtgc tctttggaaa cgtcccgcct ccctggccgg agccggagtt
     5461 gaaaatcccc gacggccttc cggaccaggc gcggcagaag atcatgagca ttctggcggc
     5521 ggcgcagccg gcgtcggcgc cggccgcgag ccgtccatcc ggcccgcagc ctttgcctgg
     5581 cggtagcgac tgatgtggcg tgccataaca cagcgtctgc tggaatggac cgaggcgcct
     5641 acgcttttga tagcgctgcg ctatgccacg gagtatgccg cacttcggct ctggagcctc
     5701 gtcatcggct gctttccgat agagacgaat ttggcgacgg ggcgcttgat gggccggata
     5761 tggtggcttg cgaagcgttc gcatcgcgag cgggcccttg aaaacctgcg gcattccttg
     5821 ggcgatcgct ataccgaacc agaactgcgg cggatcgcac ggcgttcgtt cgagcacttc
     5881 gcgcaacttt atctggtgga gttatggctg actccgcgga ttatcaacaa gtggtcctgg
     5941 gcgcgctatg tagagttgca tcagcttggc ccggctttac gcgagctgct gagtgagcgc
     6001 ggcgtgataa tggttaccgc gcatttcggc aacttcgagc tgcttggttt cacgatttgc
     6061 cggctgggta ttccgctggt ggcggtgatg cggcccttgg acaatccgct tgtcaatcgc
     6121 cacctggtgc gggcgcgcca ggcgggcggg ttgtcgctgc tctataagaa gggcgcaacg
     6181 cagtcggccg agtcgattct tgcatccggc ggcacgctgt gtttcatcgg cgaccaggac
     6241 gccggccgca aaggcatttt tgtagacttc ttcgggcgtc cggcttcgac ctacaagtcg
     6301 atcggcctgc tggcgatgca gcagcgcgta cccattatcg tcggagcggc gacgcgcgtc
     6361 ggccgcggtt tccgctatcg cattgccgtg cagcggatca tccagccgga agaatgggac
     6421 cagcaggcgg atccgctgcg ctgggtcaca cagacctttt cgcatgctat ggaaggcacc
     6481 atccggcagg cgccggagca gtacctctgg gtgcaccgcc gctggaagca ccagcctgca
     6541 ccgcgcggtg cacagcggca tcacgcggca gactcggcgt cagcacgaac cggctcggcg
     6601 ccggcgcgca cttgagcgcg tgcttgagaa ggacgctatc cacccctact ttaacttgtc
     6661 ttcacaagtt gccgtctccg gataggccgc catcatttcc accagc
//
LOCUS       DAOSYS010000196         6702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9698_length_6702_cov_5.629607, whole genome shotgun sequence.
ACCESSION   DAOSYS010000196 DAOSYS010000000
VERSION     DAOSYS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9698_length_6702_cov_5.629607"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLU99_11690"
     CDS             <1..331
                     /locus_tag="PLU99_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17756.1"
                     /translation="DLWSHELGYLEFRYFDGAAWRWTWELTEGNPLPHLIQITVGFDS
                     ITQAELDGKDQDTYPIELFPLGDDLPHPDRYAMIVRIPAADQFFSNTLQRVGQDMGEQ
                     FGVEEVY"
     gene            331..2289
                     /locus_tag="PLU99_11695"
     CDS             331..2289
                     /locus_tag="PLU99_11695"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOO17757.1"
                     /translation="MARRGGFILPIVLIVISLLAVTMASFVFFVRAEVHGTTAHADAQ
                     QALLAAESGLNEVIATLRLEPHNAAAWFDSPERFRHKLIWCELYERDRDPVAKTGMRE
                     LSLEGGGAPPQAWRFSVVADRDRDPSGLDNKSMRFGITPESGKLNLNTADEAQIAALL
                     NDVLSRLPQVENVPELINALLDWRDADNDTREGGAESDYYNTLEPPYNAKNGPFDSIE
                     ELLLVKGFNAAILYGEDVNRNGILDENENDGPESFPPYDNHDGTLDRGIAHYLTICSR
                     EPDTALDNKPRINLALDAATITAQIEESFTPEELEICQGAVDFILQLKGQNFDFTRLK
                     HVAELYREGLQPDEGATDVDAALAASPITLEQLPYILDRFSVPRSAQRSPQEHEEEQG
                     PPSEESHEDEEGEGEEAGQGDQERDNETPAQPQDSGPRQPRPRSQGQPSANPDSQGER
                     AAREREQDSNLPENANPKERGRRQAIRPGQEEDEEQSGGEELEAVLGQLQGGGLTQGL
                     GQPQGQQQEQGIPGLINVNTASARVLSLIPGITPEAVEAIIAARPALTPTAARTPAWL
                     LSAGVDTATFHAIAPFITTKAYQYRVEVLGYADHNRVVKRCEWIIEMVGPVAQVRYFR
                     DLTALGLGWPIDDEYVAGDAAQNEARGG"
     gene            2313..3905
                     /gene="pilM"
                     /locus_tag="PLU99_11700"
     CDS             2313..3905
                     /gene="pilM"
                     /locus_tag="PLU99_11700"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOO17758.1"
                     /translation="MIRLSRKMLCLDWDHRSLRLVVAKIAGGRAELEDAHSHRIPNTV
                     DPDNPDALGQFIQQMMRRHRWSHKRVVVHIPRDRAVINRLLLPPTPDHELAAAVRFQA
                     MKELPFPLEDAVIDYVIKRRDENGLAVEVLLAAVTQDVLGGVQATCEAAGLEPLRIGL
                     RPYANLISVKHLAGLGQKRVLFVDVGPRMTEIDVMDSGTLAFARAANVTTPMAGDSAT
                     EDSRITTFADVADMAASDEAVETAVNELAVEVTRTLQAYRASEPEVVVHEILVAGGTG
                     VEPHLLTALGKRLGVRATLFDPTEALRVSADEAVKLRSFSAALGLAWGLSEAGAFELD
                     FLNPKKPIPPRAALKRRLRIGGMAAAAVVVAALSIDLTLYFRAHNDYKLQRAAADKLR
                     KELATYIGIKHRVEEVNEWCVGAIWPEHLLQLTEHAVSPGKEMLVQQLELNSSKALAS
                     MNKIQATRWEVATEFANKLDEATGADGQRLYKATRDTWRTQNLRDSKFKGTTNINLEL
                     LELKKQRELQEKRAKERRRELP"
     gene            3930..5123
                     /locus_tag="PLU99_11705"
     CDS             3930..5123
                     /locus_tag="PLU99_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17759.1"
                     /translation="MNPREKKLAIAMGVLLAAAGLWVVVKYGVVNRWRTLQANIAKAQ
                     FDAERYRKNLEAARAAEADWSALRPLSHNVDRAQNKFLQDLHQLLDKNGLAEDCTVTP
                     QAPRELAKTKGMTEVRATIAARGTLQQIVNFLCDFHERDYLARLDRIKLAAEQKPPSA
                     TRSPAPSTPSKSSSRRRGSRSTAAATRESLGYGPEGPMLNATIEASALVLAELKIGNN
                     VVAQPTAEAGEDAEPVERLPRTREEYARIWETNIFKLWQEPPPPPPPDVVAAEAPTPQ
                     PEPEPQYQEPPPPARPNKRIGATISMSGKLAVLILDCDDPGKPCEKVEINQPVDDGTL
                     VLVLPGGNGGMVVQVPEQRGLKYYFYPFGHDETGRQYTFADRREIDPRQDAEIQAELN
                     KVLRG"
     gene            5307..>6702
                     /locus_tag="PLU99_11710"
     CDS             5307..>6702
                     /locus_tag="PLU99_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO17760.1"
                     /translation="MTRQNFRRGLIRLVLSALVTMLAVDLVLAQAGPSRPARSGRSSR
                     GSAKPPASQPLDPEAQEKLRKLMEMSPQHAVPPPPGEAPDQKAPEAPAAPSKKRPERK
                     PAASPGLDELNRRARASTQKASAPATPVSETPADDEEDDFFGPPYPPATPTIDKPRAG
                     ALKTDNEKERAPTPRTSSATDDMEWFNFEQMPWEDVVRVFADRLGKPLLGFDDLVIGG
                     ELTYSSDRKFTKEEALDELNLLMHMQGYRFVETEHHIEVVPLSEMGQRVPVSKTYPSR
                     SAFEEADPPRMDYVIVYLQIKDVPARKVQYAFETMFSDDFNIFTLEDSNQLKLTGAAQ
                     DVHKFLDLMDRIDIDENDPRQMRIFQIQTNARDIERMVREILDLAGGGAAPKRMEALE
                     QIRRGARGRAATPVPAPTPSPSSVDTEVRMSADDRTNTLIVRATPDKLERIAGLIEQI
                     DKRPELEFYTQVTEI"
BASE COUNT         1347 a         2191 c         2153 g         1011 t
ORIGIN      
        1 cgatctgtgg tcgcacgagt tgggatacct cgaattccgc tatttcgacg gcgcggcctg
       61 gcgctggacg tgggagctga cggaaggcaa cccgcttccg cacctgatcc agatcacggt
      121 cgggttcgac tccattacgc aggccgagtt ggacggcaag gaccaggaca cctacccgat
      181 cgagctgttc ccgctcggcg acgatctgcc gcatcccgac cgctacgcca tgatcgtgcg
      241 cataccggcc gccgatcagt ttttctccaa cacgctgcag cgcgtcgggc aggacatggg
      301 cgagcagttc ggcgtcgagg aggtgtactg atggcgcggc gcggcgggtt cattctgccc
      361 atcgtgctga tagtgatcag cctgctggcg gtgacgatgg ccagcttcgt gttcttcgtg
      421 cgtgccgaag tccacggcac gaccgcgcac gccgacgcgc aacaggccct gctggcggcc
      481 gagtccgggc tgaacgaggt gatcgcgact ttgcggctcg agccgcacaa cgccgcggcc
      541 tggttcgatt cgcccgagcg cttcagacac aagctgatct ggtgcgaact ctacgagcgc
      601 gaccgcgacc ccgtggccaa aacgggcatg cgcgaactgt ccctcgaagg gggcggcgct
      661 ccgccgcagg cctggcgctt ttccgtggtg gccgaccgcg accgcgatcc cagtggtctc
      721 gacaacaaga gcatgcgttt cggcattacg cccgaatccg gcaagctgaa cttgaacacg
      781 gctgacgagg cgcagattgc cgcgttgctg aacgacgtac tgtcgcgctt gccgcaggtc
      841 gagaacgtgc ccgagttgat caacgcgctg ctggactggc gcgacgcgga caatgatacg
      901 cgcgagggcg gggccgagtc cgactattac aacacgctcg agccgccgta caacgccaag
      961 aatggaccgt tcgattccat cgaagagctg ctgctggtca aaggctttaa cgcggccatc
     1021 ctgtacggcg aggacgttaa ccgcaacggc attctcgatg agaacgagaa tgacgggcct
     1081 gagtcgttcc cgccctacga caaccacgac gggacgctcg atcgcggcat cgcccattac
     1141 ctgacgatct gctcgcgcga gccggacacg gccctggaca acaagccgcg gatcaacctg
     1201 gccctggatg ccgccaccat cacggcccag atcgaagaat ctttcacgcc cgaggaactg
     1261 gaaatctgcc agggcgccgt cgatttcatc ctgcagctca aggggcagaa cttcgacttc
     1321 acccggttga agcatgtcgc ggaactgtat cgcgaggggc tgcagccgga cgaaggggcg
     1381 acggacgttg atgcggcact ggccgccagc ccgatcaccc tggagcaact gccctatatt
     1441 ctggaccgct tcagcgtgcc gcgatcagca cagcggtcac cgcaggagca cgaggaagaa
     1501 caaggaccgc cctcggaaga gtcgcacgag gacgaagagg gcgagggcga agaagcgggt
     1561 cagggcgacc aggaacgaga caacgaaacg cccgcccagc cgcaagactc cggcccgcgc
     1621 cagccccggc cgcgcagcca agggcagccg tccgccaatc ccgactctca aggtgaacgc
     1681 gcggcgcgcg agcgcgagca ggattccaat cttcctgaaa acgccaatcc gaaagagcga
     1741 ggaaggcgtc aggccatcag accaggtcag gaggaagacg aggaacaaag cggtggcgag
     1801 gagcttgagg cggttctggg gcagcttcag ggcgggggcc tgacgcaagg tctgggccaa
     1861 ccccagggcc aacagcagga gcagggaatc ccggggctga tcaatgtcaa caccgcttca
     1921 gcccgcgtgc tgtctttgat tcccggcata acgcccgagg ccgtcgaagc catcatcgcc
     1981 gctcgccccg cgcttacgcc gacagccgcg cgtacgccgg cctggctgct gtcggccggg
     2041 gtcgacaccg ctaccttcca cgcgattgcg ccgttcatta ctaccaaggc ctaccaatac
     2101 cgcgtggaag tgctcgggta cgcggatcac aaccgggtgg tcaagcgttg cgaatggatc
     2161 attgaaatgg tcggcccggt ggcgcaggtg cgctacttcc gcgacctgac cgccctgggc
     2221 ctgggctggc ccatagatga tgaatacgta gccggggatg cggcccaaaa cgaggcccgc
     2281 ggcggctaaa gcttgtgcca aggagacgtt tggtgataag gctctcgcgc aagatgctgt
     2341 gtctggactg ggatcaccgc tcattgcgcc tggtcgtggc gaagatcgcc ggcggccggg
     2401 ccgagttgga agacgcccat tcccaccgca tcccgaatac ggttgacccg gacaacccgg
     2461 acgcgctcgg gcaattcatc cagcagatga tgcgccgcca ccgctggagt cataagcgtg
     2521 tggtcgtgca catcccccgc gaccgcgccg tcatcaaccg cctcctgctg ccacccaccc
     2581 ccgaccatga gttggccgcc gctgtgcgct tccaagccat gaaggaactg cccttcccgc
     2641 ttgaagatgc agtgattgac tacgtcatca agcgccgcga cgaaaacggc ctggcggtgg
     2701 aggtgctcct ggccgccgtt acccaggatg ttctgggcgg cgtgcaggcc acgtgcgagg
     2761 cggccggcct ggaaccgttg cgcatcggcc tgcggcccta tgcgaacctg atcagcgtca
     2821 agcacctggc cgggctgggg cagaagcgcg tcctgttcgt ggacgtcggg ccgcgcatga
     2881 ccgagatcga cgtgatggac agcggcacac tggccttcgc gcgggccgcg aacgtcacca
     2941 cgcccatggc cggcgactcg gccaccgagg actcgcgcat cacgaccttc gccgacgtgg
     3001 ccgacatggc cgcctcggat gaagcggtcg agacggccgt caacgagctg gcggtggaag
     3061 tcacgcgcac gctgcaggcc tatcgggcca gcgagcccga ggtggtggtt cacgagattc
     3121 tcgttgccgg tggcacgggg gtcgagccgc acctgctgac cgcgctgggc aagcgcctcg
     3181 gtgtgcgggc cacgctgttt gatccgaccg aagcgctgcg agtctcggcc gacgaagccg
     3241 ttaaactgcg ttccttctcg gcggctttgg gcttggcgtg gggcctgagc gaggcgggcg
     3301 cgtttgagct ggattttctg aaccccaaga agccgatccc gccgcgggcg gccctgaagc
     3361 gccgcctgcg cattgggggc atggcggcgg cggcggttgt agttgcggcg ctgagcatcg
     3421 atctgacgct ttatttccgc gctcataatg actataagct tcagcgagcg gcggcggaca
     3481 agttgcgcaa ggagttggct acctacatcg gaatcaagca cagggtggaa gaggtgaacg
     3541 agtggtgcgt cggcgctatt tggcccgagc acctgctgca gcttaccgag cacgcggtca
     3601 gccccggcaa ggaaatgctg gtccagcagc tcgagcttaa ctcctccaaa gcgctggcca
     3661 gtatgaacaa aatacaggcc acacgctggg aggtggccac cgaatttgcc aacaagctgg
     3721 atgaggcgac cggagctgac ggccagcgcc tctacaaggc gacccgggac acctggcgta
     3781 cgcagaacct gcgcgacagc aagttcaaag gcaccaccaa catcaatctc gagttgctgg
     3841 agctcaagaa gcaacgcgag ttgcaggaga agcgggccaa ggaacggcgg agggaactgc
     3901 cttagcgccg gcgaactagc ggagaacgca tgaatccacg cgagaaaaag ctggcgattg
     3961 cgatgggcgt cctgctcgcg gcggccggcc tctgggtcgt cgtcaagtat ggcgtcgtca
     4021 atcgctggcg gacgctacag gccaacatcg ccaaagccca attcgatgcc gagcggtacc
     4081 gcaagaatct ggaagcggcc cgcgccgccg aggcggactg gtccgccttg cggccgttgt
     4141 cgcacaatgt cgaccgcgcc cagaacaagt tcctgcagga tctgcaccaa ctgctcgaca
     4201 agaacgggct ggcggaagac tgcacggtta cgccgcaggc gccgcgcgag ctggcgaaga
     4261 ccaagggaat gaccgaggtg cgggcgacga tcgccgcgcg cggcactctg cagcagatcg
     4321 tcaatttcct gtgcgacttc cacgagcgcg actacctggc gcgcctcgat cgcatcaagc
     4381 tcgctgcgga acaaaagccc cccagcgcca cccgcagccc cgctcccagc acgcccagca
     4441 agtcctcgtc gcgccgtcgc ggttcgcgca gcaccgccgc cgccacgcgc gaaagcctcg
     4501 gctacggccc cgagggcccc atgttgaatg cgactatcga ggcctcggcc ctcgtgctgg
     4561 cggaactgaa gatcggcaat aacgtcgtag cgcagcccac ggccgaggcc ggcgaggacg
     4621 ccgaaccggt cgagcgcttg ccgcgaacgc gcgaggagta cgcgagaatc tgggaaacca
     4681 acattttcaa gttatggcag gagccgcccc cgccgccgcc gccagatgtg gtcgcggccg
     4741 aggcgcccac gccccagcct gaacccgagc cgcaatacca agagccgccc cctccggccc
     4801 gcccgaacaa gcgtattggc gcgacgattt caatgtccgg gaaattggcg gttctaatct
     4861 tggactgcga cgacccgggc aagccctgtg agaaagttga gatcaatcag cccgtagatg
     4921 acggaaccct ggtgctcgtg ctacccggcg gcaacggtgg catggtggtc caggttcccg
     4981 agcagcgcgg actcaagtac tacttctacc cgtttggcca cgacgaaacg ggccggcagt
     5041 acactttcgc cgatcgccgc gaaatcgatc cgcgccagga tgccgagatt caggccgagc
     5101 tgaacaaggt cctgcgtggc tgatacgaac gaagtgcatg aaacgcgcgc cggctgcgcc
     5161 cggccgcggg ccggctggct tgcgacgcag gcccgcccgg cgggtataac tgatgacgca
     5221 aacggcgggc gcggccgctg ttgcgcgaaa aatggtgtac aaggatccgc cttgggtccc
     5281 ataagtatcc agagagaggt taaagcatga cgcggcagaa ctttcggcgc ggcctcatcc
     5341 gcctcgttct gagcgccttg gtcactatgc tggcggtgga tttggtcctc gcccaagccg
     5401 gccccagcag gccggcgcgc agcggaagat cctcacgcgg ctccgccaaa ccaccggcca
     5461 gtcaaccgct ggacccggaa gcccaggaaa aactgcgcaa gctcatggag atgagtccgc
     5521 agcacgccgt tccgccgccg cccggcgagg cgccagatca aaaggcgccc gaagccccgg
     5581 ccgcgccgtc caaaaagcgg ccggagcgaa agcctgccgc gtcgccgggt ctggacgaac
     5641 tgaatcgccg cgcccgggcc tccacgcaga aggccagcgc ccccgccacc cccgtgagcg
     5701 aaacgccggc cgatgacgag gaagacgact tcttcggacc gccgtatccg cccgccacgc
     5761 cgaccataga caaaccgcgg gccggcgcgc tcaagaccga caacgagaag gagagggctc
     5821 ccacccctcg cacgagcagc gcgacggacg acatggagtg gttcaatttc gagcagatgc
     5881 cgtgggagga cgttgtccgc gtcttcgcgg atcgcctggg caagccgctg ctgggctttg
     5941 acgacctggt catcggcggc gagctgacgt acagcagcga ccgcaagttc acgaaagagg
     6001 aagcgctcga cgaattgaac ctgctgatgc acatgcaggg ctatcgcttc gtggaaaccg
     6061 agcatcacat tgaggtcgtg cctctcagcg agatgggaca gagagtcccc gtcagcaaga
     6121 cctacccctc gcgcagcgct ttcgaggaag cggatccgcc gcgcatggat tacgtgatcg
     6181 tctatttgca gatcaaggac gtgccggccc gcaaggtgca gtatgcgttc gagacgatgt
     6241 tctcggatga tttcaacatc ttcacgctgg aggacagcaa ccagctcaag ctcaccggcg
     6301 ctgcccagga cgtccacaag ttcctggatc tgatggaccg catcgacatc gacgagaatg
     6361 acccgcgcca gatgcggatc ttccagatcc aaaccaacgc ccgtgacatc gaacgcatgg
     6421 tgcgcgagat cctggacctc gccggcggcg gggcggcccc caagcgtatg gaagcgctcg
     6481 agcagattcg ccgcggcgcc cggggacgcg ccgccacccc tgtgcccgcg cccacgcctt
     6541 cgccgagcag cgtggatacc gaagtccgca tgagcgccga cgaccgcacc aacacgctga
     6601 tcgtgcgcgc caccccggac aagctggaac gcatcgccgg cctgatcgaa cagatcgaca
     6661 aacgccccga actggagttc tacacccagg tcaccgagat ca
//
LOCUS       DAOSYS010000197         6671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9757_length_6671_cov_4.492443, whole genome shotgun sequence.
ACCESSION   DAOSYS010000197 DAOSYS010000000
VERSION     DAOSYS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6671
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_6671_cov_4.492443"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLU99_11715"
     CDS             <1..476
                     /locus_tag="PLU99_11715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO17761.1"
                     /translation="GLPMVLLEAGAAGLPVIATGVGAIPEFLSPRPGQPGFCAREEMI
                     APIVPPGDVIALVREMTRLALEPSLRQRIGGRLQARVREGYTSEHVAQRLSAIYDRLL
                     GLTSGTLAEATPSRSVPTGRRPAQVKSETQLPTGRSRFTQTQPTSSAPGLQVQNR"
     gene            473..1903
                     /locus_tag="PLU99_11720"
     CDS             473..1903
                     /locus_tag="PLU99_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17762.1"
                     /translation="MSAYEWLVRQAVFPAHERLRGRPTLRELAALRRLARCSPQEIEA
                     TEQQRLQDLLQFAARELPFYAERFARHGVNPYAADAKAELAKLPVLHKPEVRANADRM
                     TWPAVPGGLVPCVSGGTTGDTLHFFIDRLRAAQSMAARLFMQSLFGVRPGDRRMWVWA
                     SPIELKRSRFRHWRDRLINEIVLDAFDMSAATLDAYVRRMIEYQPRAIIGYTSAVALL
                     AQHAARTCSQRDFRDLRLVVLTADEVQPEERSAAQAAFGCPVASEYGSREVGLIAHDC
                     PHGRMHIISPHLYVEITHENSRLPAGQLGDVTCTNLNTRAQPLIRYSLGDLGALSASG
                     CPCGLPFPVMEVTAARVSSFVALPGGKLCSGHIIPHLVRSDPCITHFKVYQHALDLFE
                     VLLVVDETFTPDAITRLQHRFRQYFGAAVQVQCRVVEHIPPDPSGKRRQLISAVSPEY
                     RTFELVAAPQPDGPRSAGKHLVGFGS"
     gene            1970..3661
                     /gene="murJ"
                     /locus_tag="PLU99_11725"
     CDS             1970..3661
                     /gene="murJ"
                     /locus_tag="PLU99_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOO17763.1"
                     /translation="MSETRSVVSSAKLIAICTLLSRITGLIREMLLAHSFGLRWVLDA
                     FNYGFTIPNLFRRLLGEGAMAAVFVPTFTQVLEKEGRPAAWRLLARTLALLSLALVTI
                     VIVLEAVILAIWYLAPGGDPAEQASRQLLLWLTALMLPFMLAICIVALFSAILNCLGS
                     FVPAALAPVLLNIGMIVAIAWLGPWLRPGDKVGQATVVGWMVIISGFVQVAVLVPVLR
                     RSGVRLGWSLHFRNPAVKRMLRLLAPVALGQGVLAVGVFLDAQVCVLLTHKAGTPETI
                     SWLGGAVRYPLEEGAWSAITVAQRLYQFPLGVLVISLGTAAMPSFARLAARQDWRAWS
                     LEVRQSLRLAIFEGVLTGTMMFMLAAPIIRLLYQYDRFSAADTARASWVLTFFGFAMW
                     SFCAQHIVLRGFYSFHDARTPLKISAAILPLNLLLTLVLVWFPSVREAAFAISSAITT
                     GFAVIISLIIFERRTQVRLLDRELGLALGKMAVAGAVCALTVYFTRPIAGGLPVGQVT
                     GRLISAFGPLTTGTAAFLAAAWVLGLPEVGLLLPKLRRRKAKAVQAAPAPADTEV"
     gene            3766..4350
                     /locus_tag="PLU99_11730"
     CDS             3766..4350
                     /locus_tag="PLU99_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOO17764.1"
                     /translation="MRVLAVNGSPREKGNTYLMLKRVCDPLSTAGMEIDWAYLHAAGI
                     APCGACMSCAKTKDGRCYGEDDAANELIDRTRNADVILLGSPVYFGSVTGQIKAYMDR
                     VGFVSRMGDNFLRRKIGAAVVPARRAGQLLTFAELNMWFLINGMIVPGSSYWNVGAGR
                     AEGDILKDNEALLTLDELAKNIIWLADRLRDEPR"
     gene            4599..6095
                     /locus_tag="PLU99_11735"
     CDS             4599..6095
                     /locus_tag="PLU99_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOO17765.1"
                     /translation="MHKFLLFPSERQKMLADLPGAHLVGADGIPVRGDIQLINAEIRC
                     TSRSSDPFGLSLLWSVDGFGTVQLETTRLPAREKPYLLNLELARHRLMRITLKREEWG
                     LFDYHGMDEISEMIDGARDLFIQAWGRIESPAAAAELADQSLRLSLEAGERMSQFHAA
                     VFLGRRQQSGGFAKEFVGAAVAQSWPPSLLNGPVRESVDFMRIPFVWRDVQPKEQEIH
                     FERLDAWLDACAQARLPVRGGPLLNFGVRFVPDWMYIWENDFDTILEYAREHVQRCVR
                     RYADRINTWVAVSGLHADNVFSLNFEQVMEITRMAAGAVKEVAPRAQVILDLTQPWGE
                     YSIHNQRSIPPLLFADMAVQSGVPFDAFGLQFLFGIPADGYHFRDLLQISSLIDKIAN
                     LGRPLHVTAVAVPSVVEAPGNPVAAGGSWKQPWSGEVQAEWLTAFCEISLSKPYVDSV
                     CLQGLADDEAATIPSGGLLTKDFAVKPACTRLARLIRELHGNAENTRK"
     gene            6073..6465
                     /locus_tag="PLU99_11740"
     CDS             6073..6465
                     /locus_tag="PLU99_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOO17766.1"
                     /translation="MQKTRGSESASQPAGQGQAPPDLRTGLVLALIFGAVCGGSACTL
                     WPSRAAAPPAPGRAVLRLDPNTASQTELLLLPGIGPRLSRNIIEHREGSETRPAFRAA
                     EDLDAVRGVGPAGVARLRDSLQFEPQAE"
BASE COUNT         1087 a         2105 c         2217 g         1262 t
ORIGIN      
        1 aagggcttcc gatggtgctg cttgaagccg gggccgccgg tctgccggtg atagcgaccg
       61 gcgtcggggc aattccggag ttcctcagcc cacgccccgg ccagccgggc ttttgcgcgc
      121 gtgaagaaat gattgctccg atcgtgccgc ccggcgacgt gattgcgctg gtgcgtgaga
      181 tgacgcgctt ggcactcgaa ccatcgctgc gccagcggat cggcggcagg ttgcaggccc
      241 gcgttcgcga aggctacacc tccgagcacg ttgcgcagcg cctgagcgcc atctacgacc
      301 gcctgctcgg actcacgtcc ggaacgctgg ctgaagccac cccgagccgc tctgtgccga
      361 ccggccgcag accggcgcaa gttaagagcg aaacgcaact gcccaccggg cggtcacgct
      421 tcacgcaaac tcagccaaca agttccgcgc cgggacttca ggtgcagaac cgatgagcgc
      481 ctacgagtgg ctggttcgcc aggccgtatt cccggctcac gagcgactcc gcggccggcc
      541 cacgctgcgc gagctcgcgg cgttgcgccg gctggcccgt tgctcgccgc aagagataga
      601 ggccaccgag cagcagcgct tgcaggactt gctccagttc gccgcgcggg agttgccctt
      661 ctacgccgag cgcttcgcac gccacggagt caatccgtac gccgccgacg cgaaagccga
      721 actcgctaaa ctccccgttc tgcacaagcc cgaagtacgc gccaacgccg accgcatgac
      781 ctggccggcc gtgcccggcg gcttggtgcc gtgcgtcagc ggaggaacga ccggcgatac
      841 cctgcacttt ttcattgatc gcctgcgtgc ggcccagagc atggccgcac gcctcttcat
      901 gcagagcctc ttcggcgtgc gcccgggcga ccggcgcatg tgggtgtggg cgtcgccgat
      961 cgaattgaag cgttcgcgct tccgccattg gcgcgatcga ttgataaacg aaatagtgct
     1021 cgacgcgttc gacatgtcgg cggccacgct ggacgcatac gtgcgccgca tgatcgaata
     1081 ccagccgcgc gccattatcg ggtataccag cgccgtggca ctgctggccc agcatgccgc
     1141 ccgaacgtgc agccagcgcg atttccgcga tctgcgcctg gtggtgctga cggccgacga
     1201 ggttcaaccc gaggagcgct ccgcggccca ggcggcgttc ggatgccccg tcgcgagtga
     1261 atacggcagc cgcgaggtgg ggttgatcgc ccacgattgc ccgcatggcc ggatgcacat
     1321 aatctcgccg catctgtacg tcgaaatcac gcacgaaaac tcgcgcctac cggcggggca
     1381 actcggcgac gtcacgtgca ccaacctgaa cacgcgtgcc cagcccctga ttcgctactc
     1441 tctcggcgac ttgggcgcgc tatccgcgag tggttgtccg tgcggcttgc cgtttccggt
     1501 gatggaagtg acagccgccc gcgtgtccag tttcgtcgcc ctgcccggcg gaaagctctg
     1561 tagtggccac ataatcccgc acctggtgcg gtccgatccc tgcataacgc acttcaaggt
     1621 ctaccagcac gcgctggacc tgttcgaagt gttgctggtc gtcgatgaga ccttcacgcc
     1681 ggacgcgatc acacgtttgc agcatcgttt tcgacagtac ttcggggcgg cggtacaggt
     1741 ccagtgccgg gtggtcgagc acataccgcc cgatccttcc gggaagcgcc gccaactgat
     1801 ctcggccgtc tcgccggaat accgaacgtt cgagttggtc gccgctccgc agcccgatgg
     1861 gccaaggagt gcaggcaagc acctcgttgg tttcggctcc tgagacgcgc aagatgccgc
     1921 tgccaagtgc gcgtggaagg cgctcccgcc cgcggctatc atcgcggcca tgtcggaaac
     1981 gcggagcgtc gtttcttccg ccaagctaat cgccatctgc acgctgctat cgcgcatcac
     2041 ggggctgatc cgcgagatgc tcctggccca cagtttcgga ctgcgctggg tgctggacgc
     2101 gtttaattac ggcttcacga tccccaacct gtttcgccga ctgctgggtg aaggggcgat
     2161 ggcggccgtc ttcgtgccaa cgttcacgca ggtgctggaa aaggaggggc ggccggccgc
     2221 ctggcgtctg ctggcgcgga cgctggccct tctgagcctg gcgctggtca cgatcgtcat
     2281 cgtgctcgaa gcggtcatcc tggccatctg gtatctggcg ccgggcggcg atccggctga
     2341 acaggcctca cgccagcttc tgctgtggct gacagcgctc atgctgccat tcatgctggc
     2401 gatctgcatc gttgcgctgt tctccgccat cctgaattgc ctgggcagct tcgtgccggc
     2461 cgcgctggcg ccggtcctgc tcaacatcgg catgattgtg gccatcgcgt ggctggggcc
     2521 gtggctgcga ccgggggaca aagtggggca ggcgacggtt gtcggctgga tggtgatcat
     2581 ctccggattc gtgcaggtgg ccgtgctggt accggtgctg cgccgctccg gcgtgcgtct
     2641 gggatggagc ctgcacttcc gcaatccggc ggtaaaacgc atgctgcgcc tgttggcgcc
     2701 ggtggccttg gggcagggcg tgctggcggt cggcgtcttc ctggatgcac aggtttgcgt
     2761 gctgctgacg cataaggccg gaacaccaga gacgatttcc tggctggggg gagcggtcag
     2821 atacccgctt gaggaaggcg cctggtcagc cataaccgtt gctcagcggc tttatcagtt
     2881 tccgctcggg gtgctggtga tttcgctggg cacggcggcc atgccttcgt tcgcgcgact
     2941 cgcggcgcga caggattggc gggcgtggtc gcttgaggtg cgccaatcgt tgcggctcgc
     3001 gattttcgag ggcgtcctga cgggcacgat gatgttcatg ctggccgccc cgatcattcg
     3061 cctgctttac caatacgacc gcttttctgc agccgatacg gcccgcgcga gttgggtgct
     3121 gacgttcttc gggttcgcga tgtggtcgtt ttgcgcccaa cacattgtct tgcgcgggtt
     3181 ctacagcttc cacgacgcgc gcacgccgct gaaaatcagc gccgcaattc tgccgctgaa
     3241 cttgctgctg acgctggtgt tggtgtggtt cccgtcggta cgcgaagcgg cgttcgcgat
     3301 cagctccgcc atcaccacgg ggttcgcggt gataatcagc ctgatcatct tcgagcgccg
     3361 cacacaagta cgcctgctgg accgcgaact cgggctggcg ctggggaaaa tggcggtcgc
     3421 aggagcggtc tgcgcactta cagtctactt cacgcggccg atcgcaggcg gcctgccggt
     3481 gggtcaagtc actggccggc ttatcagtgc tttcgggcca ttgaccaccg gcaccgctgc
     3541 atttctggcg gcggcctggg tgctgggatt gccggaggtc gggctgctgc tgccgaagct
     3601 gcggcgcagg aaggcgaaag cggttcaggc cgctccggcg ccggcggaca cggaggtgtg
     3661 agcgacttgg attgaggccg gcgctgcgat gcggtacatt ccggccgcgg gcttcggctc
     3721 gtggtcctgc ggcctcaatt tgtagcttat tgtggagttc tcatcgtgcg cgttctggcc
     3781 gtcaacggca gcccccgtga aaagggcaac acgtatctca tgttgaagcg ggtatgcgat
     3841 ccgctcagca ccgccggaat ggagatcgac tgggcttacc tgcacgctgc gggaatcgcg
     3901 ccgtgcgggg cctgcatgag ctgcgctaag accaaggatg gccggtgcta cggcgaggat
     3961 gatgcggcca acgagttgat cgaccgcacc cgcaacgccg acgtgatcct gctcgggtcg
     4021 cccgtgtact tcggttcggt gaccgggcag ataaaggcgt acatggaccg ggtcgggttt
     4081 gtcagccgga tgggcgacaa cttcctgcgc cgcaagatcg gagcggcggt ggttcccgcc
     4141 cggcgggccg ggcaactgct cacgttcgcc gagctgaaca tgtggttcct gattaacggc
     4201 atgatcgtgc ccggcagcag ctactggaac gtgggcgccg gccgcgcgga aggcgacatt
     4261 ctgaaggata acgaagccct cctcacgctg gatgaactgg ccaagaacat tatctggctt
     4321 gcggatcgac tgagggacga gccgcgctaa gccgtcccag gtagcgtcgc gatgtggcgc
     4381 tttaaggctc gctagcgcca gggtcgtttt gcaaagcacg gcggcccggc gcacgtggcg
     4441 agcttggccc gcttggcggc ttggcggttg cctcgggtcg cttttcgccg ccgccccagg
     4501 ggcttatcat aagaccgttt cgcctgccca ggctggccgg cacgccggga ctggtccggc
     4561 ggtgtcactg gcggaataga gctcggagca gagagcgcgt gcacaagttt ctcctttttc
     4621 cttcagaaag acagaaaatg ctggcggacc tgcccggcgc acatctggtc ggcgccgatg
     4681 gaatcccggt tcgcggcgac atccagttga tcaacgcgga gatccgctgc acctcgcgct
     4741 cctccgatcc gttcggtctt tcgctacttt ggtccgtgga cggctttggc acggtgcagc
     4801 tcgaaacgac gcgcctgccg gcccgcgaaa aaccgtactt gctgaatctg gagctggccc
     4861 gccaccgttt gatgcgcatt accctcaagc gcgaagaatg ggggctgttc gattaccacg
     4921 gcatggacga aatcagcgag atgatcgatg gggcccgcga cctcttcatc caggcctggg
     4981 ggcgcatcga gtcgccggcg gcggccgctg aattggctga ccagtcactg cgcttgagcc
     5041 tggaggccgg cgaacggatg tcccagttcc acgccgcggt ctttctgggg cggcgccagc
     5101 agagcggcgg ttttgccaag gagttcgtcg gggcggcggt cgcccagtcc tggccgccga
     5161 gcctgctcaa cgggccagtg cgcgagagcg tggatttcat gcgcattccg ttcgtctggc
     5221 gcgacgtgca gcccaaggaa caggaaatcc acttcgagcg tctggatgcc tggctggacg
     5281 cctgcgccca ggcgcgcctg ccggtgcgcg gcggcccgct gctgaacttc ggcgtgcgtt
     5341 tcgtgccgga ctggatgtac atctgggaaa atgacttcga caccatcctg gagtacgcgc
     5401 gcgagcacgt ccagcgctgc gtgcggcgct atgcggatcg catcaatacc tgggtggcgg
     5461 tcagcggcct gcacgccgac aacgtcttct cgctcaattt tgagcaggtg atggaaatta
     5521 cccgcatggc cgcaggcgcc gtgaaggaag tcgcgccgcg ggcccaggtc attttggatc
     5581 tgacgcagcc ctggggtgaa tattcgatcc ataaccagcg ctccatcccg ccgctcctgt
     5641 tcgcggacat ggccgtgcaa agcggggtgc ccttcgacgc tttcggcctg cagttcctgt
     5701 tcggaattcc cgccgacggc tatcacttcc gcgacctgct gcaaatcagc tccctgatcg
     5761 acaagattgc caatctcggc cggccgttgc acgtcacggc cgttgctgtg ccttccgttg
     5821 ttgaagcccc gggtaacccg gtcgcggcgg gcggctcttg gaaacagccc tggtcgggcg
     5881 aggtgcaggc cgagtggttg accgccttct gcgagatcag cctgtcgaag ccttatgtcg
     5941 acagcgtctg cctgcaaggg ctggccgacg atgaggcggc caccattccc agcggcggac
     6001 tgctgaccaa ggactttgcc gtcaagcctg cgtgtacgcg gctggcgcgt ctgattcgag
     6061 aactgcacgg caatgcagaa aacacgcgga agtgaaagcg catcgcagcc tgccggccag
     6121 ggacaggcgc cgcctgacct gcggaccggg cttgtgttgg cgctgatctt tggcgccgtg
     6181 tgcggcggca gcgcatgcac gctctggccg tcgagagccg cggcgccgcc ggcacccggc
     6241 cgggcggtcc ttcgtctcga cccgaatacc gccagccaga ctgaactgct gttgctgccc
     6301 gggatcggcc cgcggctcag ccggaacatt attgagcacc gcgaaggcag cgaaacgcgt
     6361 cccgcatttc gagcggcgga agacttggat gccgtacgcg gcgtggggcc ggccggcgtg
     6421 gcgcgcttgc gggattcgct gcagttcgag ccgcaagcgg aataggagac cagcgcatgt
     6481 tggctcgccg gcggcccttt tgggagtaaa caatcaaaaa acctgaacga aacagggcgt
     6541 tgaaaagtca cggcttgtgg cacaaacgcc ggcgctgctc tatattgtgc gcgtgccgac
     6601 tgaacgaaag ttgaagtgga gataccagga tgaatgcatg gctacgtcgg gctgagatga
     6661 acaagaacct c
//
LOCUS       DAOSYS010000198         6659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9778_length_6659_cov_4.616747, whole genome shotgun sequence.
ACCESSION   DAOSYS010000198 DAOSYS010000000
VERSION     DAOSYS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9778_length_6659_cov_4.616747"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..832
                     /locus_tag="PLU99_11745"
     CDS             104..832
                     /locus_tag="PLU99_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17767.1"
                     /translation="MAKSRQHTQAAGSRGQTAGAIPAAPAALSAAEQLAVEQDRRLAL
                     EAARAYQEGRVPTAAQRAALRRHEQRKETTLRWQYYRSIPQKDWRIMSGRQAKVINEQ
                     ASLYGIPFGGPVIDLPAVVRALHDFLARNARLLASGMDDASLLFGRSTPSLERVRRAT
                     AELREIQVARERGNLVDRALARECLLQLAGMLRAAGETLQRRYGPDALEIVNDMIANW
                     ERLVEEHFGAQDSSIPRGTHAGPA"
     gene            816..1310
                     /locus_tag="PLU99_11750"
     CDS             816..1310
                     /locus_tag="PLU99_11750"
                     /inference="COORDINATES: protein motif:HMM:NF025717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HOO17768.1"
                     /translation="MRGLPEWNFPAFDAAAERLRAMGFVVYNPADMSRAQGFSPDQPF
                     GPDQLRKSLQIDIWHLLYHCDRLAALPGWQHSEGARIEVALARALGMPVFALDCSDQP
                     LAAKTMAPESRKFSEAIVVPEGERFAVRGRAGPVYGEFFDRGVADDYAAYLNMTHGGQ
                     HDAR"
     gene            1478..2026
                     /locus_tag="PLU99_11755"
     CDS             1478..2026
                     /locus_tag="PLU99_11755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HOO17769.1"
                     /translation="MSRVVARANVKDMALLALVARQHHPELLEAKVNIAFVLVHPKYD
                     AQGIPQGPALTRHGHPVAGRIRLAKPADRLIGKYDALLEVDAFRWEKALQAPSREALL
                     DHKLSHLVLVRDESGEIETHADGRPKLRLRPDDWAVTGFAAVIDRHREAALEYQALEL
                     VCRGCQLTFEQLPLTVRAAGNG"
     gene            2046..4211
                     /locus_tag="PLU99_11760"
     CDS             2046..4211
                     /locus_tag="PLU99_11760"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOO17770.1"
                     /translation="MRAPGTSDIGLRDQAAQSMAVALDPLHSELRALAREARAARYRT
                     RRQWAEDEIVLPPDGPTANERFRSRRQPFTGLFLDAVDSGRWTEINMTGPSQSGKTLT
                     GSVIPVLYHLFELRENVIFGLPDENMAADKWREVLLPAIERTRYKDLIPKLGQGSRGG
                     DKFVAIKFANGATLRFMTGGGRDKQRAGFTARVLVISEADGMDEAGTTSREANKVAQL
                     KARTKAFSARTGRPPLVYSECTVSWDTGYTWQTHQRGTCTRIVLPCPHCGHWVTPERE
                     HLRGWQDAPSEVDAYEQAHFCCPACGQAWTEDARDQANRRALEAHALLHRGQEIVPLD
                     EWRRSNEGKPPELADPIRQLDEHWGIIGPLPKTFCFSLRYSAVNNILKSAGDFAAGEW
                     VAARATDQEAANREQCQFVWATPPPRPEVDVTPLDPQIVGARMHSAPRGYIPDEARWL
                     TMAIDVGKWALHWLGIAWREDATGHVFDYGIVDVHSEDRSERDIGIAVLMALREFRDR
                     VVLHGWASARGPRIPDQVWVDSRYQAPAVLAFCRESRRLPALNRECFRPTLGFGAGQQ
                     RTRPYMRPRSTGTTVLRIGEEYHFVRLKDEFVVRVEVNADYWKEWIHQRLSTPLGQAG
                     ALTLFQAMPDEHIKLSHHLTAEVRRERFVAGKGLAVTWDRVRRQNHWLDCAYNAAAAG
                     HFCGARLVPDAAALAAPVAPPPPRTLRMPDGRPFLITER"
     gene            4212..4439
                     /locus_tag="PLU99_11765"
     CDS             4212..4439
                     /locus_tag="PLU99_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17771.1"
                     /translation="MRLGGLGWQRCCKPPANWRELRCSPFCEHGRTGMEYAYSTPNGI
                     RHYRRYGEGRRAYWTKLVREILWINTERTPK"
     gene            4436..4726
                     /locus_tag="PLU99_11770"
     CDS             4436..4726
                     /locus_tag="PLU99_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17772.1"
                     /translation="MSRYRRVRTKDENGLRTASELICLEVPYQDGEPPGYATRRIDMH
                     MTPAEALTMDRVLEALRGRALPNTDRLVQSPADVVRWLLAGIRSGRERLAGE"
     gene            4723..4926
                     /locus_tag="PLU99_11775"
     CDS             4723..4926
                     /locus_tag="PLU99_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17773.1"
                     /translation="MTSRTITLTLAVRLGAGDPPALPDKLLAELRHYLEGELARHLRP
                     GELVIGFGPLGRRYGGTGAGARV"
     gene            4939..5403
                     /locus_tag="PLU99_11780"
     CDS             4939..5403
                     /locus_tag="PLU99_11780"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOO17774.1"
                     /translation="MVAETVITLWQPFALLMVLGLKRIETRSWMTGFRGRLWIHAAGS
                     EPRDCKALMHESPIRELLAQHGYHGLHELPRGAILGRVELYFVQRIGPAFLMPDLREC
                     LLGDYRCGRYAWFMRGARRLAEPIAHTGQRGMWHWTPPAGLQWAQEGDSGQT"
     gene            5390..6025
                     /locus_tag="PLU99_11785"
     CDS             5390..6025
                     /locus_tag="PLU99_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOO17775.1"
                     /translation="MAKRKTQPGAIPATVAGLEIRRMPLSQLQPGPYNPRRKLRPGEA
                     AYERLRRSMQTFGYVDPLIWNKRTGHLVGGHQRLNVLIDEGTVNEVDVSVVDLSPAEE
                     KVLNVALNGVHGEWDQPALAALLLDLRRDQSIDETLTGFEATEIDLLLQSAPIKLRAI
                     SVRPPPVMTWVLIGIPTVRYGEIAEQIDKIAEVPGILCELTANSSEVRSQS"
     gene            6038..6613
                     /locus_tag="PLU99_11790"
     CDS             6038..6613
                     /locus_tag="PLU99_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17776.1"
                     /translation="MTSGSGVETDNSALPSKLALRRHFLRAYHAGTRPLVFDACAGSS
                     VIWRTLAQEFDVQYWGVDVKPRRGRLRIDSVRILQQPGWTFDVIDIDTYGSPWEHWAA
                     MLPHVRKPTTVFLTWGNVWYGGAPGAIRSILGLTFTVKPPAMLLAKAYRYALPYLLDQ
                     ARQHGVRISEACEAPSAGSVRYFGLRLEPGV"
BASE COUNT         1204 a         1982 c         2311 g         1162 t
ORIGIN      
        1 ggccgggggg cttcccatct gggcactttt ttacacacaa cgcgtgaaat cgggagtaag
       61 taagcaatag caatggctag gcgttagagc ggcaagcgat ggaatggcca aatcacggca
      121 acatacacag gcggcgggct cacgcggcca aaccgcgggc gcaattccgg ccgcgcctgc
      181 ggcgctgtct gccgctgaac agctcgcggt cgagcaggac cggcgcttgg cgctcgaggc
      241 cgcccgggcc taccaggagg ggcgggtgcc gacggccgca caacgggccg cactgcgccg
      301 acatgagcag cgcaaggaaa caacactgcg ctggcagtac tatcggtcga tcccccagaa
      361 ggattggcgc atcatgtcgg gcaggcaagc taaggtcatc aacgagcagg cgtcgctata
      421 tggcatcccc ttcggcgggc cggtcatcga cctgcccgcg gtggtgcggg cactgcacga
      481 tttcctcgcg cgtaacgctc gcctgctggc ctctgggatg gatgacgcca gcctattgtt
      541 tgggcgttca acgccgtcgc tcgaacgggt tcgccgggcg actgcggagc tgcgggagat
      601 tcaggtggca cgggaacggg ggaacctggt cgaccgggcg ttggcccgcg agtgcctgct
      661 tcagctcgcc ggtatgttgc gcgcggccgg cgagacattg caacggcgct atgggcctga
      721 tgccctagag atcgtgaacg acatgatcgc gaattgggaa aggttggtgg aggagcactt
      781 tggagcacag gattcgagta tacctcgtgg gacccatgcg gggcctgcct gagtggaatt
      841 tcccggcatt tgacgcggcg gcagaacggc tgcgtgccat ggggtttgta gtctacaatc
      901 cagcagacat gtcgcgtgcg caggggttca gccccgatca gccatttggg ccggatcaac
      961 ttcgcaagag cttgcagatt gacatttggc atctgctata tcactgcgac cgcttggcgg
     1021 cgctaccggg ctggcagcat tcggaggggg cgcgtattga ggtcgccctg gcacgagcgt
     1081 tgggcatgcc ggtattcgcg ctagattgtt cagaccagcc gcttgcggcg aagacgatgg
     1141 cgcccgagag ccggaaattc tccgaggcga tcgtggtacc ggaaggtgaa cgttttgccg
     1201 tgcgcggtcg ggccgggcca gtctatggcg agttctttga ccgcggcgtg gcggatgact
     1261 atgcggccta cctgaacatg acgcatggag gacagcatga cgctcgctga gatacggcga
     1321 tacatgacct cttggcagcg cgagccggcg gtggttcggc tggctagtgt tagccgggcg
     1381 gcctggcggg cgcggcagat cagcatccag cgaggcgcag cgcattcgca atataatctc
     1441 tctcagcatc tactgcgggg gccggaggag tacgacgatg tctagggttg tcgctagggc
     1501 caacgttaaa gatatggcgc tgcttgcgct cgttgcgagg caacaccacc ccgaactact
     1561 ggaggcgaaa gtcaacatcg cgtttgtgct cgtgcatccc aagtacgacg cccaaggcat
     1621 cccgcagggg ccggcgctca cgcgccatgg gcatccggta gcgggccgga tccggctggc
     1681 taaaccagcc gatcgactga tcggcaagta tgacgcgcta ttggaagtgg acgcctttcg
     1741 ctgggaaaag gcattgcagg cgccttctcg cgaggcgctg ctggatcaca agctgtcgca
     1801 cctggtgctg gtgcgggacg agtccggaga gatagagacc cacgccgacg ggcggccgaa
     1861 gctgcgcctg cggcccgatg attgggcggt gaccggcttc gccgcggtga tcgatcgaca
     1921 tcgcgaggca gcgctcgaat accaagcgct cgagctggtg tgccgggggt gccaactgac
     1981 gtttgaacaa ctgccgctta cggtccgtgc ggccggcaac gggtaaggac gcggtagggt
     2041 acgccatgag agccccggga acgtcggaca tcggcctccg cgaccaggcc gcccaatcca
     2101 tggcggtcgc gcttgatcca ttgcacagtg aactgcgcgc cctggcccgt gaggcccggg
     2161 ccgcacgcta tcgcacgcgt cggcagtggg cggaggacga gatcgtgctg ccccctgacg
     2221 ggcccacggc taacgagcgc ttccggtcga gacgacaacc tttcaccggc ctgttcctgg
     2281 atgcagtcga cagcggccgg tggaccgaga tcaatatgac cggcccctcg cagagcggga
     2341 aaactctgac cggctcggtg atcccagtgc tctaccacct gtttgagttg cgcgagaacg
     2401 tgattttcgg gctgcccgac gaaaatatgg ctgcggacaa gtggcgcgag gtcctcctgc
     2461 cggccataga gcgcacgcgg tacaaggacc tgattcccaa gttgggacag ggcagtcgtg
     2521 gcggcgacaa attcgtggcg atcaaattcg ccaatggcgc gacattgcgc tttatgactg
     2581 ggggcgggcg ggacaaacag cgcgccggct tcacggctcg cgtgctggtc atcagcgagg
     2641 ctgatggcat ggacgaggcc ggcacaacta gccgcgaggc taacaaggtt gcgcagctca
     2701 aggcccgcac caaagcattc tccgcgcgga ccggtcggcc gccgctggtc tactccgagt
     2761 gcacggtcag ttgggacacc ggttacacct ggcagacgca tcagcgcggc acatgcacgc
     2821 ggatcgtcct gccctgcccg cattgcggcc attgggtgac gccggaacgc gagcacctgc
     2881 gcggctggca ggacgcgccg agcgaggtcg acgcctacga gcaggcccat ttctgctgtc
     2941 cggcctgcgg ccaagcgtgg accgaggacg cacgcgacca ggccaaccgg cgcgctctag
     3001 aggcgcacgc actgctgcac cgcggccagg agattgtgcc gctcgatgag tggcgacggt
     3061 ccaatgaggg caagccgccg gaactggccg acccgatccg gcagctagat gagcactggg
     3121 ggatcatcgg cccgttaccg aagacgttct gcttttcgtt gcgatactcg gcggtcaaca
     3181 acattctgaa aagcgccggc gacttcgcgg ccggtgagtg ggtcgcggcc cgcgccaccg
     3241 accaggaggc cgctaatcgc gagcagtgcc agttcgtctg ggcgactccg ccgccgcggc
     3301 cggaggtgga cgtcacgccg ctggatcccc aaattgtggg cgcgcgcatg cacagcgcgc
     3361 cgcgcggcta catacccgac gaggcccgct ggctcacgat ggcgatagac gtaggtaaat
     3421 gggccctgca ttggctgggc attgcgtggc gcgaggacgc gaccggccac gtatttgact
     3481 acgggatcgt ggacgtgcac agcgaggacc ggtccgagcg ggatatcggt attgcggtcc
     3541 tcatggcact gcgtgagttt cgcgatcggg tcgtgctgca cggctgggcc tcggcgcgcg
     3601 ggccacgcat ccccgaccag gtctgggtag acagccggta ccaggcgccg gccgtgttgg
     3661 cgttctgccg cgaatcacgc cgactgccgg cgcttaaccg ggagtgtttc cgtccgacac
     3721 tcgggtttgg tgccggccag cagcgcacgc ggccctatat gcggccgcgc tccactggca
     3781 ccactgtgct gcggatcggc gaggaatatc attttgtcag actaaaggat gagtttgtcg
     3841 ttagggttga ggttaatgcc gactattgga aggagtggat ccatcagcgg ctgagtactc
     3901 cgctggggca ggccggcgcg ctgaccctgt ttcaggcgat gcccgatgag cacatcaagc
     3961 tgagtcatca tttgacggct gaggtgcggc gcgagcggtt cgtcgccggc aaggggctgg
     4021 ccgtgacatg ggaccgggtg cgacggcaga accactggct ggactgtgcg tataacgccg
     4081 cggccgcggg tcacttttgc ggcgcgcggc tcgtgccgga tgcggcggca ctggccgcgc
     4141 ctgtcgcgcc tccgccaccg cgcacgctgc ggatgcccga cggccggcca tttttgatta
     4201 cggagcgtta agtgagactg ggcgggctag gctggcagcg gtgttgcaaa ccgccggcca
     4261 attggcgtga actacggtgc tcgccgttct gcgagcatgg gcgtaccggc atggagtacg
     4321 cctattcgac gcccaacggg atccgccact accgccgcta cggcgagggg cggcgagcat
     4381 actggaccaa gctggtacgc gagatattgt ggatcaacac ggaaaggacg ccgaaatgag
     4441 ccggtaccga cgcgttagga cgaaggacga aaacgggctt cgcactgcat ccgaactcat
     4501 ctgtctggag gtgccatacc aggacggcga gccgccgggc tacgccaccc ggcgtataga
     4561 tatgcacatg actccggccg aggcgctgac catggaccgg gtgctcgagg cactgagggg
     4621 ccgggcactg ccgaacacgg atcgattagt gcagtcgccc gcggacgtgg tgcgttggct
     4681 gctcgcggga atcaggagtg ggcgggagcg tttggcaggc gaatgacctc gcgcacgatc
     4741 acgctcacgc tcgcggtccg gctcggcgcc ggcgatccgc cggcactgcc agataagctg
     4801 ctggcggagc tacggcacta cctcgagggc gagctggccc ggcatttgcg gcctggcgag
     4861 ctggtgatcg gcttcggacc gctgggacgg cggtacggcg gaacaggcgc gggcgcgcga
     4921 gtgtgatgag ggacgggcat ggtggccgaa acagtgatta cgctctggca gccgttcgcg
     4981 ctgctgatgg tactcggact caagcggatc gaaacacgct cctggatgac cggcttccgc
     5041 ggccggctgt ggatccacgc agccggcagc gagccacgcg attgcaaagc actaatgcat
     5101 gagtcgccca tacgtgagct gctggcccag cacggctacc acgggctgca tgagctgccg
     5161 cgcggggcga tcctcgggcg ggtggagttg tatttcgtcc aacgcattgg gccagcgttt
     5221 ctgatgccgg atctgcgaga atgtctgctg ggtgactacc gctgcgggcg ctatgcctgg
     5281 ttcatgcgcg gcgcgcgccg cctggccgaa ccgatcgcgc ataccggcca gcggggaatg
     5341 tggcactgga ctccgcccgc gggtctgcaa tgggcacagg agggtgatag tggccaaacg
     5401 taagacacag cccggcgcga tccccgcgac ggtcgcgggc ctcgagatcc gccggatgcc
     5461 gctgagccag ttgcagcccg ggccgtacaa tccgcgccgc aagctgcgcc cgggcgaagc
     5521 cgcttatgag cggctgcggc gcagcatgca aacattcgga tatgtcgatc cgctcatctg
     5581 gaataagcgt accggccatc tggtcggcgg tcatcagcgg ctcaacgtcc tgatcgatga
     5641 gggtacggtc aatgaggtgg acgtcagcgt agttgatctg tcgcccgcag aggagaaggt
     5701 gctcaacgtc gcgctgaacg gcgtgcacgg cgagtgggac cagccggccc tagcggcgct
     5761 gctgctcgat ctacggcggg atcagtcaat cgacgagacg ctgaccggtt ttgaagcaac
     5821 tgagatcgac ctcctcctac agagcgcgcc gatcaagctg cgtgcgatat cggtgcggcc
     5881 gccgccggtg atgacctggg tattgatcgg catccccacc gtgcggtatg gcgagatcgc
     5941 cgagcagatc gacaagatcg ccgaggtgcc cggcatattg tgcgagctga ctgccaacag
     6001 ctcggaggtt agaagccagt cgtgacccgg tggtgccatg accagcggta gtggagtaga
     6061 gacagacaat agcgcattgc cgtccaagct cgcattgcgg cggcattttt tgcgcgcgta
     6121 ccacgccggc acgcgcccat tggtgtttga tgcctgcgcg ggctctagtg ttatctggcg
     6181 tactcttgcg caagagttcg atgtgcaata ctggggcgtt gacgtaaagc cgcggcgtgg
     6241 tcggctcaga atcgacagcg tccgcatctt gcagcaaccg ggctggacgt ttgacgtcat
     6301 cgacatcgac acatatggct cgccctggga gcattgggcg gcgatgctgc cgcacgtccg
     6361 caagccgact actgtgtttc taacttgggg caacgtgtgg tacggcgggg ctccgggcgc
     6421 gatccgcagt atcttggggt taacgtttac ggtgaaacct ccggcgatgc tgctggcgaa
     6481 ggcttatcga tacgccttgc cgtatctgct cgatcaggcc cggcagcatg gcgtgcgcat
     6541 aagcgaggcc tgcgaggcgc catcggccgg cagtgtccgc tattttgggc tgcggctgga
     6601 gccgggagta taaataaggc cgcacattat aattgccttt ggaggtcagc gctggcata
//
LOCUS       DAOSYS010000199         6624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9835_length_6624_cov_5.266707, whole genome shotgun sequence.
ACCESSION   DAOSYS010000199 DAOSYS010000000
VERSION     DAOSYS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9835_length_6624_cov_5.266707"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..596)
                     /locus_tag="PLU99_11795"
     CDS             complement(288..596)
                     /locus_tag="PLU99_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17777.1"
                     /translation="MVTDLQWELPTDRSNMLVAAIGTDNIHRYAMPLPSGENAALFYL
                     KNVSVSNLIEFLSQMSIKQVNSQATGGTYTLKLSGPQANYTGVDIYWNGLLVHNVDLR
                     "
     gene            1390..2466
                     /locus_tag="PLU99_11800"
     CDS             1390..2466
                     /locus_tag="PLU99_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOO17778.1"
                     /translation="MNTDLAKEVENFKQAIRNDNGTQKHADLTGLRVTKFIEGCGWRT
                     VNDITLARATEYVAARRTSDGDDHISEGTSNHYMTALSEFCSFLKSTNKLDRNPVEGW
                     KHLPRADRETRRALNHDEQHWLLSVTATSADRRHMTGWEREMVYKTALGRGFRSEELA
                     CLTPESFHLQGSTPFVYLPPDETKNSKAANQPITPELAAELREYLEEREPGLPVFPLP
                     ETRARSRLVRTDLAAAREAWIEAATTEQDKAERRKSNFLKPVTRDGFVDFHALRHTYG
                     TNLALAGVAPKVTMDLMRHSDINLTMRLYAHTELADRASGVHMLPRVGFSLGSGLGSE
                     AERLGSNVNTAERKASKRARHKSA"
     gene            2496..2570
                     /locus_tag="PLU99_11805"
     tRNA            2496..2570
                     /locus_tag="PLU99_11805"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2531..2533,aa:Met,seq:cat)
     gene            2751..2822
                     /locus_tag="PLU99_11810"
     tRNA            2751..2822
                     /locus_tag="PLU99_11810"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2783..2785,aa:Gly,seq:gcc)
     gene            complement(2991..4334)
                     /gene="aceA"
                     /locus_tag="PLU99_11815"
     CDS             complement(2991..4334)
                     /gene="aceA"
                     /locus_tag="PLU99_11815"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOO17779.1"
                     /translation="MIKATVPAAAALQKSWETDPRWKGVTRRYTAEDVLRLRGTLQIE
                     HTLARVGAEKLWRLMHEEEFVSALSAVTGNQAVQQVQAGLKAIYISGWQVAADANLAQ
                     QTYPDQSLYPADSVPALVRRVNYALMRADQIDHAAGKTGTDWFVPLVADAEAGFGGNL
                     NAFELMKALIEAGAAGVHYEDQLSSAKKCGHMGGKVLVHTGHAIEKLTAARLAADILG
                     IPTVLIARTDAFSAKLLTTEIDERDHPFIENNERLAEGFYAYRGGLEAAIARGVAYAP
                     YCDVVWCETSEPSLEDARRFAEGIHKAHPGKLLAYNCSPSFNWKANLDDATIAKFQRE
                     LGAMGYKFQFVTLAGFHALNLSMFKLALDYRREQMAAYARLQEEEFALARSDGFAATT
                     HQRFVGTGYFDEVQKAIAGDDTPTAALTGSTEEAQFAAVDKPAPVRARPAESRTR"
     gene            complement(4350..5954)
                     /gene="aceB"
                     /locus_tag="PLU99_11820"
     CDS             complement(4350..5954)
                     /gene="aceB"
                     /locus_tag="PLU99_11820"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HOO17780.1"
                     /translation="MTSITSPTLVDNLEINAPVTGEQKRILTPEALNFVVRLCRAAEP
                     TRRQLLDRRLQRQAELDAGQLPDFLPETRSVRESDWRVAPVPADLQDRRVEITGPVER
                     KMIINALNSGARVFMADFEDSNSPTWANIVNGQVNLRDAVRRTITFEQPDGKKYALND
                     EIATLMVRPRGWHLSEKHVLLDGQPISGALFDFGLYFFHNARQLLETGSGPYFYLPKL
                     ENHHEARLWNEVFIMAQDELGIPRGTIKATVLIETILASFETEEILYELRDHSAGLNC
                     GRWDYIFSFIKRFRSRPDFVLPDRAQITMTVPFMRAYALNVIKSCHRRGAHAMGGMAA
                     QIPIKNDPVANEAALAKVRADKEREVNDGHDGTWVAHPGLVPVAMEAFSRMRGPNQIA
                     RPREDVKVSAADLLRVPEGQITEEGLRTNIRVGIQYLEAWLRGNGCVPLYNLMEDAAT
                     AEISRTQVWQWIKHPRGVLDDGRKVTAELFSALTADELARLRTTLGPQAFHDGKFDLA
                     TKIFDRISTADELVEFLTLPAYEYLD"
     gene            complement(6227..>6624)
                     /locus_tag="PLU99_11825"
     CDS             complement(6227..>6624)
                     /locus_tag="PLU99_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019500384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOO17781.1"
                     /translation="RDPTRLITDETRMYLRDLQDHTIQIVDLVETYRDIASGITEVYL
                     SSVSQHTNEIMKVLTIIATIFIPLTFIVGVYGMNFDSSASPLNMPELRWYYGYPLLWL
                     LMLLIATAMLLLFRRYGWLGGGREELPGE"
BASE COUNT         1296 a         1993 c         2047 g         1288 t
ORIGIN      
        1 ggcgtgatat gtcgcttacg ggacatgaca ctatccttct ctgcacaggc tgacgagcgt
       61 tcgcgtgttg gccatcgcca tctgagcgtt cgccatcgct atttcgcaga gcaagcggtt
      121 cttggcccgc agggtttcga cgtgcgcggc gcgcaatgaa atggcagaga tcggtgctat
      181 cggcgactca agcgcaccgg gggactggcc ggaccgcgcg caacagcccg acagtccccc
      241 ggcgcaaacg accaatgcta ctaatcggta cgtgttcacc aatcagatca gcgcaagtcc
      301 acgttatgaa cgagcaggcc gttccaataa atgtccactc cggtgtagtt cgcttgaggc
      361 ccagacaact tcaaggtgta ggtgccgccg gtggcctggg aattcacctg cttaatgctc
      421 atctgcgaaa ggaactcgat caggttggac acgctcacgt tcttcaggta gaagagcgcg
      481 gcgttttctc cagagggcaa cggcatggcg taccgatgaa tattatccgt gccaatcgcg
      541 gcgacaagca tgttgctgcg gtccgtcgga agctcccact ggagatcagt taccatgtag
      601 aaatctagca cgtcgttttt tgtgaaatcc caagtgctgt tcgcgaggtt tgtgaaccac
      661 atcaaaggtg cgaccttacc ttcggtatca ggtaccaggc cgtaatccgc tcggctatat
      721 ccctggagtg accccagcgt agcttccgac ggccagtatg gcccggggtc aaaccctggc
      781 gggtttcctt caatgtagac tctcgcaccc gacccgtcca agtagtacca ctggcctcgg
      841 gccggatcat accagtagca gaacccgtct tggcagaatt ttccaaacgg aataccctct
      901 tccggtgtcg gcggatcgac aatcactggc ggctgggggg gtgacgggtg gcctgcccgg
      961 cgtggtctca cacgcccaca aggcaagaac agtgacgacg gaaactgcca gaaacagctt
     1021 tctcaacatt gcgaactcct ccgaaaacat gagtagggag gagccccacg cgggcctcct
     1081 gtcgagaaca gatgcactgt acgtctcatg cctctcccag ccttagacga tccgcaacgc
     1141 cgttcacaga tcggccgaat tcggatcgaa accgcgcacg aaggctatca atcgacgcca
     1201 agtcgcaacg ggcacagaag catgtaccct cgaaattcaa cctatggggc aggaattgtc
     1261 tcgtcaatgt cttttctgga ctttgcgcaa aagcgcacag tgccccaaac aaggtccacc
     1321 gtgtcctgct agcccgcccc taacagcgct tggcggcaag acgggcagaa agggcgggac
     1381 gacaacgcag tgaatacaga cctggccaaa gaagtcgaga acttcaagca agccattcga
     1441 aacgacaacg gcacacagaa gcacgcagac ctgaccgggc tgcgcgtaac gaagttcatc
     1501 gagggctgcg gctggagaac ggtgaacgac attacgctgg cgagggcgac ggaatacgta
     1561 gcggctcgcc gcacgagcga cggagacgat catatatccg agggcacaag caaccactac
     1621 atgaccgcgc tgtcggagtt ttgctcgttc ttgaagagca cgaacaaact cgaccgcaac
     1681 cccgtcgaag gttggaagca cctgccccgt gcagaccggg agacgcggcg ggcgctcaac
     1741 catgacgagc aacactggct gctgtcggtg acggccacga gcgccgatcg ccggcacatg
     1801 accggttggg agcgcgaaat ggtgtacaag acggcgctcg gtaggggctt ccgttcggaa
     1861 gagcttgcct gcctgacgcc ggagagtttt catctgcaag ggtcaacgcc gttcgtctac
     1921 ctgcccccgg acgaaacgaa gaacagtaag gcggcgaacc agcccattac gcccgagctg
     1981 gctgccgagc tgcgcgagta cctggaagag cgggagccgg ggctaccggt ctttccgttg
     2041 ccggagacac gcgcccggtc gcggctcgtt cggaccgacc tggcggcggc acgagaggcg
     2101 tggatcgaag cggcgacgac ggagcaggac aaagccgaaa ggcgaaagtc gaacttcctc
     2161 aagcccgtaa cccgagacgg cttcgtggac ttccacgccc tgcgccacac ctacgggacg
     2221 aacctggcgc ttgcaggcgt cgcgccaaaa gttacaatgg acctgatgag acacagcgac
     2281 atcaacctga ccatgcggct gtacgcccat accgagctgg ccgaccgggc ttccggcgtc
     2341 cacatgctgc cgcgcgtggg gttcagtttg gggtcaggtt tggggtcgga ggctgaacgt
     2401 ttggggtcaa acgtgaacac ggctgaacga aaggcgtcaa aacgtgcccg ccataagtca
     2461 gcctagaatc gagatttaca gcgtttttac gctgcggtgg cgtagctcag ttggttaaga
     2521 gcggcggatt cataacccgc aggtcgacgg ttcgactccg tccgccacca ttttttcagc
     2581 cgtgccgtgg gcgagttccg ggcggcgtcc ggagccgctg attcgaggcg tcgcgcacag
     2641 cctgcgcatc cggctgcgac ggatcgcgga cgaatctgcg gcggaggttt tttggccccc
     2701 tttgcaggcc ggcgacggac gatataatag cggcctcaaa aagaggcgac gcgggtgtag
     2761 ctcagtggta gagcgtcacg ttgccaacgt gaatgtcgtg ggttcaagtc ccatcacccg
     2821 cttttcctaa accacgccga aggctgattt cggcgtgcct gtccgagatg gggcggcgta
     2881 gcaagcaaac cgacggtccc ttccaacagc agccttccgg cgaccgtggt cagagccgtg
     2941 aagcgttctt tgcgtcgtac tcgtgcagtc ggcccagcaa aactcgcagt ttagcgcgtg
     3001 cgtgactctg ccggtcgcgc gcggaccggg gccggtttgt ctacggccgc gaactgcgcc
     3061 tcctcggtcg aaccggtcag ggccgcggtg ggcgtgtcgt cgcccgcaat ggccttctgt
     3121 acctcgtcga agtaacccgt gccgacgaag cgctggtgcg tcgtcgcggc gaacccgtca
     3181 ctgcgggcga gcgcgaattc ttcttcctgc agccgggcat aggccgccat ctgctcgcgc
     3241 cggtagtcca gcgccagctt gaacatgctc aggttcagcg cgtggaagcc ggccagcgtg
     3301 acaaactgga acttatagcc catcgcgccc agttcgcgct ggaacttcgc gatcgttgcg
     3361 tcgtcgagat tcgctttcca gttaaacgat ggtgagcagt tgtaggccag cagctttccc
     3421 ggatgcgcct tgtgaatgcc ttccgcaaag cggcgggcat cttcgagact gggctcggac
     3481 gtctcgcacc agaccacgtc gcagtagggc gcgtaggcca cgccgcgcgc gatggccgcc
     3541 tccagaccgc ccctgtacgc gtaaaagccc tcggccaggc gctcattgtt ctcaatgaac
     3601 gggtgatcgc gctcgtctat ctcggtagtc aagagcttgg cactgaatgc atcggtgcgc
     3661 gcgatcagca cggtcggaat gccgagaatg tcggccgcca gccgggccgc ggtcagtttc
     3721 tcgatggcat gccccgtatg caccagcact ttgccgccca tgtgcccgca tttcttggcc
     3781 gaagagagct ggtcctcgta atgcacgccg gccgcgcccg cctcaatcag ggctttcatc
     3841 agctcgaacg cattgaggtt gccgccgaaa cccgcctcgg cgtcggccac cagcggaacg
     3901 aaccaatcgg tgccggtctt gcccgccgcg tggtcgatct gatcggcgcg catcagggca
     3961 tagttgacgc gccgcaccag cgccggcacg ctgtccgcag ggtatagact ctggtcgggg
     4021 taggtctgct gagctaggtt ggcgtcggcc gcgacctgcc agccgctgat atagatggcc
     4081 ttcaagccgg cctggacctg ctgaacggcc tggttcccgg tcacggcact gagggcgctt
     4141 acgaactcct cctcgtgcat cagccgccat agcttttccg cgcccacgcg ggcgagcgta
     4201 tgttcgattt gcagtgtgcc gcgcagccgt agaacgtctt ccgcggtata ccgccgggtc
     4261 acgcccttcc agcgcgggtc ggtttcccag ctcttttgca gcgccgcggc tgccgggaca
     4321 gtcgccttga tcatgggttg ctccttgaat cagtccaggt actcgtaagc cggaagcgtg
     4381 agaaactcga ccagttcgtc ggctgtactg atgcggtcaa aaatcttggt tgccaggtcg
     4441 aatttgccgt catggaaggc ctgcggtccg agcgtggtac gcagacgcgc cagttcgtcg
     4501 gcggtcaagg cgctgaacag ttcggccgtg accttccgcc catcatccag cacgccgcgc
     4561 gggtgcttga tccattgcca gacttgcgtg cgcgaaatct cggccgtggc ggcatcttcc
     4621 atcaggttgt acagtggcac gcaaccattg ccgcgcagcc aagcctcgag gtattggatg
     4681 ccgacgcgga tgttggtgcg caggccctct tccgtgatct ggccctccgg cacgcgcaac
     4741 aggtccgctg cgctaacctt tacgtcctcc cgcggccgtg cgatttggtt cggcccgcgc
     4801 atccgcgaaa acgcttccat tgcgacaggg accaggcccg ggtgcgctac ccatgtgccg
     4861 tcgtggccgt cgttgacctc gcgctccttg tcggcacgga ccttggccag tgccgcttca
     4921 ttggccaccg ggtcgttctt gatgggaatc tgtgcggcca tgccgcccat ggcatgcgcg
     4981 ccgcgcctgt ggcaactctt aatgacgttc aaggcgtacg cccgcatgaa cggtacagtc
     5041 atggtgatct gcgcccgatc gggcaggacg aaatcgggcc gcgaccgaaa gcgcttgatg
     5101 aaactgaaga tatagtccca gcggccgcaa ttcagtccgg cagagtggtc acgcagctcg
     5161 tacagaattt cttcggtttc gaaggaggct aatatggttt cgatcaagac cgtggccttg
     5221 atggtcccgc ggggaatgcc cagttcatcc tgggccatga tgaagacttc attccacagg
     5281 cgggcttcgt gatggttctc cagcttcggt agatagaagt aaggcccact gccggtctca
     5341 agaagctgcc gcgcgttgtg gaagaagtag aggccgaagt cgaacagtgc gccggagatc
     5401 ggctgaccgt ccaacagtac gtgcttttcc gacaaatgcc agccgcgcgg gcggaccatg
     5461 agtgtggcga tctcatcgtt cagggcgtac ttcttgccgt ccggttgctc gaaggtgatg
     5521 gtgcgccgca cggcgtcgcg cagattgact tgtccgttaa cgatgttggc ccaggtcggc
     5581 gagtttgagt cctcgaaatc ggccatgaag acccgcgcgc cgctgttcag cgcgttgatg
     5641 atcattttcc gttcgaccgg gccggtgatc tcgacgcgcc ggtcctgaag gtctgccgga
     5701 accggcgcga cgcgccagtc gctttcgcgg acagagcggg tttcgggcaa gaaatccggc
     5761 aactgcccgg cgtctaattc cgcttggcgt tgcaagcggc ggtccagaag ctggcgccgc
     5821 gtcggctccg cggcccggca caggcgaacc acgaaattca gggcctctgg ggtaagaatg
     5881 cgtttctgtt cgcctgttac gggcgcgtta atctcgaggt tatccacgag cgttggtgac
     5941 gttatcgagg tcattgaaca gttccttcaa ctaaccgcga agtaaggtag ggcggcgtcg
     6001 cagccgcgcg gccgaagtac gcgaagtgtg gagcggcggt gtagattctt cgaaagccac
     6061 ggttcagcgg gatgcgcggc cttgatttcg gctctggcac agtaattccc atccgtcggg
     6121 gcagccggtg aacagcctta ttatagttgc cgcactcttt cgagtcggga ataggcggat
     6181 tgctgcaagc cgacgctgag gtgacgagct tttcagcgcc gcctgctcat tcgcctggca
     6241 gttcttcgcg ccctccgccc aaccagccat agcgccggaa cagcagcagc atggcggtcg
     6301 cgataagcag catcagcagc cacagcagcg gatagccgta gtaccaacgc agctccggca
     6361 tgttgagggg cgaggcgctg gagtcgaagt tcattccata gacgcccaca atgaacgtga
     6421 gcggtatgaa tatggtcgca atgatcgtca gtactttcat gatctcgttc gtgtgttgac
     6481 tgacgctcga cagatagacc tccgtgatgc cggaggcaat gtcacgatat gtctcaacca
     6541 ggtcgacgat ctggatggtg tggtcctgga gatcgcgcag gtacatacgg gtttcgtccg
     6601 tgatcagccg ggtcgggtcg cgca
//
LOCUS       DAOSYS010000200         6608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_9864_length_6608_cov_5.842515, whole genome shotgun sequence.
ACCESSION   DAOSYS010000200 DAOSYS010000000
VERSION     DAOSYS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9864_length_6608_cov_5.842515"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PLU99_11830"
     CDS             <1..1044
                     /locus_tag="PLU99_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOO17782.1"
                     /translation="NTGYGGEIKKGVFTIMNYLLPMQGVMAMHCSANVGDAGDVALFF
                     GLSGTGKTTLSADPKRRLIGDDEHGWSDEGVFNFEGGCYAKVIRLSAEHEPQIWQAAH
                     SFGTILENVAWDPASRRIDLDSDIMTENTRASYPVSLIPGVVPEGYVKSHPKNVIFLT
                     CDAQGVMPPIARLDANQAMYHFISGYTAKIAGTEIGLGIEPQITFSACFGAPFMVHHP
                     FYYAKLLADKMAKHKCNCWLVNTGWVGGRFGVGKRISIRHTRNLLNAALEGKLVEVKY
                     RKDKLFGFDVPLSCPDVPDDVLEPSTAWGNKDDYWKTYDGLAARYIENFKLYKEGCPP
                     EVAAAGPKRLSRL"
     gene            1120..2478
                     /locus_tag="PLU99_11835"
     CDS             1120..2478
                     /locus_tag="PLU99_11835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOO17783.1"
                     /translation="MAGEQGLCVSATAREVRRAPRFVRLLCLLALLGGVWLFARAGNT
                     TAKSGWPDFEFFYKAGVSLLERGSLDAGYDLTPEGQLQARGRLDWYLPFTSRLMTLLA
                     WMPQRQAGQVWLALNVVALLATVRLVGRHFTGLPAPDWAVTQLLPVAALALFWWWEFR
                     LLQINNLTLLLMVGSFVSWRSGRPKTAGLWLGLAVLIKIVPLLLVVWFALKRQFRTVA
                     AALATIVLAGPVADAVVFGASDAGDIYRSWVRAAVHEASHSGLIAAQREMDWRNQGMG
                     AVMCRWLHPTNYNTRFDNDPRQRPAPEVYRLNVANLSRQQVSAVVLVIEGLLLGGLLW
                     LGRRGARGLNVWALRVEWALFVTAMLWFMPVMRSYHVIWAYPLVALLAGAIHHHGVAH
                     WWSLIAWAALLGLVGSQATAVWMESGAGGAILGAVLGLGVPAVLLRRRVGGAVQSKES
                     ER"
     gene            3023..3286
                     /locus_tag="PLU99_11840"
     CDS             3023..3286
                     /locus_tag="PLU99_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17784.1"
                     /translation="MLYEEARAHGHWVTRLCAAGFLLMVEDPKARTRAINRLRDWETE
                     YARASASQIRAFVEGAESAMQRGARGSRPAPTARPAKKKAKRG"
     gene            complement(3877..5553)
                     /locus_tag="PLU99_11845"
     CDS             complement(3877..5553)
                     /locus_tag="PLU99_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOO17785.1"
                     /translation="MSCKVARLGASTPLAADNAAPPEARRNGDPTTMSGIPIKSLYTL
                     DDIGLEALQQLGLPGEFPYLRGMHKDMYRGRIWTMRQFAGMGNARHTNERFKYLLEHG
                     QTGLSTAFDLPTLMGRDSDDALSLGEVGKCGVAISSLEDMKVLFDGINLGEVSTSMTI
                     NAPAAILLAFYICVARSQGVALDRLRGTLQNDILKEFHAQNEFVFPPSPSVKLVIDTI
                     EYCTQHLPQWNTVSISGYHIREAGATAPQELAFTIADGMCYTEESIKRGLDVDAFAPR
                     LSYFWDVHNEFFEEIAKIRAARRLWATIMRDRFGAKNERSLWCRCHCQTAGVSLTEQQ
                     PMVNIIRVAYQALAAVLGGTQSLHTNSMDETLALPTENAVKIALRTQQIIAEETGVTR
                     TVDPLGGSFFIESLTNEVQARAQAIIDEIYNRFGGVLPATEAGYFRRAIADSSARYGA
                     EFDRGERVMIGVNKYVEKDYERIPVLKIDEEVEREQVARLKELKARRDADPASRERHA
                     KALKALREAAASGENVMPALIEASEAMATVGEMMHTLEGVYGRYDGGPEL"
     gene            5786..6304
                     /locus_tag="PLU99_11850"
     CDS             5786..6304
                     /locus_tag="PLU99_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOO17786.1"
                     /translation="MSLFRLPKPTSMTLNLAPMVDVLMCLIIFFLLASKLVTAEHFPV
                     KLPWAVAAREVETHDLGARVTINVRRADGADEHAEYVVVDWNGRDIAERVLQPGELVG
                     LLRSRAARAAAENQPVRCVIRADEDVQYQHVETVLRACGLAKVGNIVFSVNKGSQPEA
                     ASVMHLGVEGGG"
     gene            6304..>6608
                     /locus_tag="PLU99_11855"
     CDS             6304..>6608
                     /locus_tag="PLU99_11855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOO17787.1"
                     /translation="MRRFEPARDFAPNLAPMVDVIMVLLVFFLLGASLNLVKQGVLQT
                     ELDPRSGPGGGTAVEVNPRIRIALRAAGDAQGVGAETYAPRILLMDDALPENTFESLR
                     "
BASE COUNT         1090 a         1916 c         2321 g         1281 t
ORIGIN      
        1 aacaccggct acggcggcga aatcaagaag ggcgtcttca ccatcatgaa ctacctgctc
       61 cccatgcagg gcgtgatggc catgcactgc tccgccaacg tcggcgatgc cggcgacgtg
      121 gccctgttct tcggcctgtc cggcacgggc aagaccacgc tctcggccga ccccaagcgc
      181 cgcctgatcg gcgacgacga gcacggctgg agcgacgagg gcgttttcaa cttcgagggc
      241 ggctgctacg ccaaggtcat tcgcctgtcg gccgagcatg agccgcagat ttggcaggcg
      301 gcccactcgt tcggcaccat actcgagaac gtcgcctggg atcctgcttc gcggcggatt
      361 gacctcgaca gcgacatcat gaccgagaat acacgggcct cgtacccggt cagcctgatc
      421 cccggcgttg tgcccgaagg ctacgtcaag agccacccga agaacgtcat cttcctgacc
      481 tgcgacgccc agggcgtgat gccgccgatc gcccgcctgg acgcgaacca ggccatgtac
      541 cacttcatca gcggttacac ggccaaaatc gcgggcaccg aaatcggcct gggcatcgag
      601 ccgcagatca cgttcagcgc ctgcttcggc gccccgttca tggttcacca cccgttctac
      661 tacgccaagc tgctggccga caagatggcc aagcacaagt gcaactgctg gctggtgaac
      721 accggctggg tcggcgggcg tttcggcgtc ggcaagcgca tcagcatccg ccacacgcgc
      781 aacctgctga acgcggcgct ggaaggcaag ctggtcgagg tgaaataccg caaggacaag
      841 ctgttcggct tcgatgtgcc gctgagctgc ccggacgtgc cggacgatgt gctcgagccg
      901 tccaccgcct ggggaaacaa ggacgactac tggaagacct acgacggcct ggcggcccgc
      961 tacattgaaa acttcaagct ctacaaggaa ggctgcccgc cggaagtggc cgcggcaggc
     1021 ccgaagcggc tgtcgaggct gtagcagcga tagtgttttg cgaatagcgc ggcgttgacg
     1081 caatcgccgg ccgggtacaa tgggcccgca atggaggcga tggccggcga gcaggggctc
     1141 tgcgtttctg caacagcgcg ggaggtgcgc cgcgctcccc gttttgtcag gctgctttgt
     1201 ctgctcgccc tgctgggcgg ggtttggtta ttcgcgcgcg ccggcaacac gaccgccaag
     1261 tcggggtggc cggatttcga gtttttctat aaggccggag tgagtctact ggagcggggg
     1321 agcctggatg cgggctacga cctgacgcct gaggggcagt tacaagcgcg tgggcggctg
     1381 gactggtacc tgccgttcac gtcgcgtttg atgacgctgc tggcgtggat gccgcagcgg
     1441 caggcgggcc aagtctggct ggcgctgaac gtggtggcgc tgctggcgac ggttcggctg
     1501 gtggggcggc actttactgg gctgccggcg ccggactggg cggtcacgca gttgttgccg
     1561 gttgcagcgc tggcgctgtt ctggtggtgg gagttccggc tgcttcagat caataatttg
     1621 accctgctgc tgatggtggg gagctttgtg agctggcgct cgggacgccc caagacggcg
     1681 gggctgtggc tcgggctggc ggtgctgatc aagatcgtgc cgctgctgct ggtggtatgg
     1741 ttcgcgttaa agcgccagtt ccggacggtg gcggcggcgc tggcgacgat cgtgctggcg
     1801 ggaccggtgg cggacgcggt ggtgttcggc gcaagcgacg ctggtgacat ttaccgctcg
     1861 tgggtgcgcg cggccgtcca tgaggcatcg catagcgggc tcatagcggc gcagcgcgag
     1921 atggactggc gtaatcaggg gatgggggcg gtgatgtgcc gctggctgca cccgacgaac
     1981 tacaacacgc gcttcgacaa cgatccgcgg cagaggccgg cgcccgaggt ttaccggctg
     2041 aacgtggcca acctgtcgcg gcagcaggtt agcgcggtgg tgctggtcat cgaggggctg
     2101 ctgttggggg ggctattgtg gctagggcgt cgcggggcgc ggggactgaa cgtgtgggcg
     2161 ctgcgtgtag agtgggcgct gttcgtgacg gcgatgctgt ggttcatgcc cgtgatgcgc
     2221 agttatcacg taatttgggc gtatccgttg gtggcattgt tggcgggggc gattcaccat
     2281 catggcgtgg cgcactggtg gtcgttgatt gcgtgggcgg cgctactggg gctggtgggc
     2341 agccaggcca ctgcggtgtg gatggaatcg ggggctggcg gagcaattct gggcgcggtg
     2401 ctggggctgg gcgtgccggc agtgctgttg cggcggcgcg tagggggagc agtgcaaagt
     2461 aaagagtccg aacgttagaa ggcgtgtccg cgcggcggaa gcttcgtcaa agcgaccacc
     2521 gcgcggcggg gtggtaggta cggaacgaag agtttcggat cggcggctcg tgattgctgc
     2581 gtacggcgga atgcggcgac ggccggatgc tgcgtgaaat gcccgcgcgc ggcgtgcgcg
     2641 gttgcatgag agtagtggcg cgggcatcgg ctccccagca gcacccgacc gccgcagcgt
     2701 tccgcggatg gtgcgggtgg gcgtgcgggc tggcaccgct ttcggtgtag gcgcatgcgt
     2761 tcctgcactg cttgaagctg ctgcgcagga tgcgaagcgg cgtgcgcgtt tgcactgctt
     2821 gaagctgcgc tttcaagcag tggcacacgg ggcgcttcag gccgtggcac accgagtgat
     2881 agatgttcta tgggctggac aaagtgcgct gacaacgtaa tactcgtttt gttctaggga
     2941 agtgtgcttc ccttgaagga gctgactcat gccgcaacgc cgccgaccac agatcaactt
     3001 ccaagtcgat gacgctctga agatgctgta cgaagaggcc cgggcgcatg gtcactgggt
     3061 gacgcgcctg tgcgccgcgg gctttctgct gatggtggaa gacccgaaag cccgcacccg
     3121 ggcgatcaac cgcttgcgcg actgggaaac cgaatatgcg cgcgcttcgg caagccagat
     3181 tcgggccttt gtggaaggag ctgaatccgc gatgcagcgc ggcgctcgag gtagtcggcc
     3241 agccccgaca gctcgcccgg cgaaaaaaaa ggcaaagcga ggctgatgcg gccgagctga
     3301 gtttcgagct tcaagctgcg cgtttcgagt tccagccttt ggcaggggcg gtcagaggtc
     3361 tggtacacat aggcgtatgt ccggagttga gggttttcgg cttggcgagc cgcgcgttca
     3421 caaaccgatc ccacattgaa tgagcggcta caaaccggtc ccacactgca aacaggtcct
     3481 ggaggcggtt gggttttgag tttcggattt cgagtttcgc gaacagacga gcgctttgtt
     3541 ttggtctgtt tgtgtgacat gtttgcctcc ttgagcctaa gagtagctcg acgagcacgc
     3601 tgattagcga gccaagagcg tcacacgagc cgcacgccga tccgtggcgt ccgattccct
     3661 ggccagccag gcccgcgagt tcgaggtggc attcgcccga gcgaatcttc tgttagaagt
     3721 gtacggtatt tgttagggta atgcaagggg cgcacggaat ttttttggca gcgagggaaa
     3781 taggacctat agggcctata ggacctattt gctccgcggg gtagcacttc ccttgacccg
     3841 tgccgggcgg ctcggcgcgg tggcgctacc ggctggttac agctccgggc cgccgtcgta
     3901 gcggccgtag acgccttcca gcgtgtgcat catctcgcct accgtcgcca tggcctcgga
     3961 cgcctcgatc aacgccggca ttacgttttc accgctggcc gcggcctcgc gcagtgcctt
     4021 cagcgccttg gcgtggcgtt cgcggctggc cgggtcggcg tcgcggcggg ccttcaactc
     4081 cttcagccgc gcgacctgtt cgcgttcgac ctcttcgtcg atctttagca ccgggatgcg
     4141 ctcgtagtcc ttctcgacgt acttgttgac gccgatcatg actcgctcgc cacgatcgaa
     4201 ttcggcaccg tagcgagcgg acgagtcggc gatcgcacgg cggaagtagc cggcctcggt
     4261 cgcgggcagg acgccgccga agcggttgta aatctcgtcg atgatggcct gggcgcgggc
     4321 ctgcacctcg ttcgtcaggc tctcgatgaa gaagctgccg cccagcggat cgacggtgcg
     4381 cgtgacgccg gtttcttcgg cgataatctg ctgcgtgcgc agggcgatct tgaccgcgtt
     4441 ctcggtgggc agcgccagcg tctcgtccat gctgttggtg tgcaggctct gcgtgccgcc
     4501 cagaaccgcg gccagggcct ggtacgcaac gcggataatg ttgaccatcg gctgctgctc
     4561 ggtcagcgag acgccggccg tctggcaatg gcagcggcac cacaggctgc gttcgttctt
     4621 ggccccgaag cggtcgcgca tgatggtcgc ccacaggcgc cgcgccgcgc ggattttggc
     4681 gatttcctcg aagaactcgt tgtggacgtc ccagaagtag ctcaggcgtg gagcgaaagc
     4741 atcgacgtcc aaaccgcgct tgatggactc ttcggtgtag cacatgccgt cggcgatcgt
     4801 gaacgccagt tcctgcgggg ccgtcgcgcc ggcttcgcga atgtgatagc cactgatgct
     4861 gacggtgttc cactgcggca ggtgctgcgt gcaatactcg atagtgtcga ttacgagctt
     4921 gacactgggc gaaggcggaa agacgaattc gttctgagcg tgaaactcct tgaggatgtc
     4981 gttctgcagc gtgccgcgca gccggtcgag cgctacgccc tgtgaacgag ccacgcaaat
     5041 gtagaacgcc agcaggattg cggccggggc attgatggtc atgctggtgc tgacctcgcc
     5101 caggttgatc ccgtcgaaga ggactttcat atcctccagc gagctgatgg ccacgccaca
     5161 cttgccgacc tcgcccaagg agagcgcatc atcggagtcg cggcccatga gcgtgggcag
     5221 gtcaaacgcg gtcgacagcc cggtctgccc gtgttcgagt agatacttga agcgctcgtt
     5281 ggtgtgccgc gcgttgccca tgcccgcgaa ctggcgcatg gtccaaattc ggccgcggta
     5341 catgtccttg tgcatcccgc ggaggtaggg gaattcgccg gggaggccca actgctggag
     5401 ggcctccagg ccgatgtcat ccagagtgta cagcgacttg atcgggatgc cggacatcgt
     5461 ggtcgggtcg ccgttgcggc gcgcctcggg cggcgcggcg ttgtcggcgg ccagtggggt
     5521 actcgcgcct agtcgagcta ctttgcagct catgtaaaca tctcctgccc ggtgtgtagg
     5581 ttcgtcccgc acggcgactg gccgtggcag gtgcccgcac gtgcgatcgc gcggatagac
     5641 ctctggaatt cagaggctgc cattctatcc gatttaagcg ggccaacaaa acccggcaag
     5701 tacgctggct gcccccggtc tttttgggga ggcggcggat tgcggcctcg ccgcggctgc
     5761 tttagaactt cacctggtgc agaaattgtc cctgttcaga ttgcccaagc cgaccagtat
     5821 gacgctcaac ctggccccta tggttgacgt gctgatgtgc ctgatcatct tcttcctgct
     5881 ggcgagcaag ctggtcaccg ccgagcactt tccggtcaag ctgccatggg ccgtggcggc
     5941 gcgcgaggtc gagacgcacg acctcggggc gcgggtcacg ataaacgtgc gccgggcgga
     6001 cggggcggat gagcacgccg aatacgtggt ggtcgattgg aacgggcggg atattgccga
     6061 gcgcgtcctc cagccgggcg agttggtcgg cttgctgcgc agccgggcgg cccgggctgc
     6121 ggccgaaaat cagccggtgc ggtgcgtcat tcgtgcggat gaagacgtgc aataccaaca
     6181 cgtcgagacg gtgctgcggg cctgcggcct ggcaaaggtt ggcaacatcg ttttcagcgt
     6241 gaataagggc agtcagccgg aggccgcgtc tgtcatgcac ctgggcgttg aggggggtgg
     6301 ctgatgcggc gtttcgagcc ggcccgcgac ttcgccccga atttggcgcc catggtggac
     6361 gtgatcatgg tgctgctggt ctttttccta ctcggggcgt cgttgaacct cgtcaagcag
     6421 ggtgtgttgc agactgagct ggatccgcgc agcggccccg gcggcgggac ggcggtcgaa
     6481 gttaatccgc gtatccgtat cgcactgcgg gcagcggggg acgcgcaggg ggtcggtgcc
     6541 gaaacctacg cgccgcgcat cctcctcatg gatgatgcgc tgccggagaa cacgtttgag
     6601 agtttgcg
//
LOCUS       DAOSYS010000201         6525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10029_length_6525_cov_5.794436, whole genome shotgun sequence.
ACCESSION   DAOSYS010000201 DAOSYS010000000
VERSION     DAOSYS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_6525_cov_5.794436"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLU99_11860"
     CDS             complement(<1..530)
                     /locus_tag="PLU99_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOO17788.1"
                     /translation="MKLWAADLHIHTALSPCAADEMTPPAIVQAALERELSMIAICDH
                     NAAGNAEAAAAASLCAGRTVAGLKLAVLAGMEIMTAEEVHILGIFPSPAAAAAAAKEV
                     RATLPEATVAYHQKFGQQLVIDSGGSIVDSEPKMLAMASGFDLSAAVRLIHRHEGLAI
                     AAHVNRPSFSVFSQLG"
     gene            complement(669..1031)
                     /locus_tag="PLU99_11865"
     CDS             complement(669..1031)
                     /locus_tag="PLU99_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOO17789.1"
                     /translation="MTLAEIAERLGLQNLTPEISPAAADVTGGYVSDLLSDVLANAPK
                     GGVLVTVQVHLNVIAVAVHAELAAIIFASDRVPDDTVQAKAKEEGIALYCSKASAFEV
                     VGRLWEMGVRGPRQSDTK"
     gene            complement(1028..2650)
                     /locus_tag="PLU99_11870"
     CDS             complement(1028..2650)
                     /locus_tag="PLU99_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO17790.1"
                     /translation="MTSVSYQIEGGNYETGGAASRQIKEQLKKIGADPAVVRRAMVAA
                     YEAEMNVVIHSHGGELRAELDEHELRVDVIDTGPGIPDVEQAMKPGFSTASAKARELG
                     FGAGMGLPNIKKNSDHFAIESAAGRGTRVSFTIHLRPQALYGAGQFSIQVEAEKCRRS
                     LHCLRACPTQALRVLRGKPEILDYLCIDCTECIGACPSGAWGLRSAGEELKPSPGSIL
                     ILPPVSLVQFGAGITAGQVLTELAYLGFSDIRVTAAWESALRKAVMQYAAGETRVRPV
                     ISPVCPAVVNLIQLRFPSLLPHVAPFLSAQEAAYESVVGEDRRLLSLVPCPCQLTIVR
                     SRDRPVEKRSHVLAVLPATFAAVLHPRLKRGTGRSADTGSTPAAPDPLGVLRVTGIHK
                     VRQVLERLEDGLLTDITVIEPYACFAGCFGSPLFTEVASVAQYRWALAPAKPDPRARA
                     VRRVVPLEPRHGLRLDADMTRAIQKLARIDKLRRSLPGSNCGNCGAPTCAALAEDIVL
                     GRAQLDACVRQRMDNEPAAKQSRPGPPAEESK"
     gene            complement(2647..2997)
                     /locus_tag="PLU99_11875"
     CDS             complement(2647..2997)
                     /locus_tag="PLU99_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17791.1"
                     /translation="MKLATLAEKIGARLLTPGKTLNVAADQVYAGDRISDLLNAASEH
                     VLMVTNLASGQLTMMAGLLDVPCICLVNSQMPDETMLRSAREHGTAILVSSCGLFETC
                     GRIYRCLAEEDHLP"
     gene            complement(3232..4485)
                     /gene="hydF"
                     /locus_tag="PLU99_11880"
     CDS             complement(3232..4485)
                     /gene="hydF"
                     /locus_tag="PLU99_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOO17792.1"
                     /translation="MQNAPKGLRLHIGIFGRRNVGKSSLLNALTRQEVSIVSACAGTT
                     TDPVEKPMELLPIGPVLFIDTAGVDDIGALGQQRVERTRRIFDRTDVGLIVAEAGEWG
                     AFEEGILNELAGRNVPAIAVFNKCDLASPSPEVLARLNERKIRHVQTVAGRGDGLADL
                     REALVRSAPDDFVNTPAIIGDLVPPGELALLVVPIDKEAPKGRLILPQVQTIRDLLDH
                     EAFCMVVKERELRQAIDRLRRPPALVVTDSQAFLKVSADTPLEVPLTSFSILFARFKG
                     DLTEMVRGATAIDRLRPGDPILIAEACSHHPIGEDIGRVKIPRWLTQYVGGELEFTTT
                     QGHDFPPDLAEYKLVIHCGACVQNRREVLSRILKCRRAGVPITNYGLAIAYSLGIFER
                     ALGPFPAALEAYRAALAEGGGASPR"
     gene            4801..5055
                     /locus_tag="PLU99_11885"
     CDS             4801..5055
                     /locus_tag="PLU99_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17793.1"
                     /translation="MDSDVSNLECDFYAGGPPETDAAVNAGAAARPYIGVLFDCCGVY
                     VRVYRRPEQNEYSGRCPNCLRPVKVRVAPDGTNSRIFRAR"
     gene            5390..5578
                     /locus_tag="PLU99_11890"
     CDS             5390..5578
                     /locus_tag="PLU99_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17794.1"
                     /translation="MRSDMQRFWKVFAPLSCGGVTLGLLDAFGMFYWSNILVEVLSSL
                     FIALFGAFLGQQAGGLFA"
     gene            5732..>6525
                     /locus_tag="PLU99_11895"
     CDS             5732..>6525
                     /locus_tag="PLU99_11895"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HOO17795.1"
                     /translation="MQLPGMPGIPGAMEDPVLREKAEKEAEHLLEDLKKEVKADIKDI
                     GTLRKELHITVPAKVIADHLDHNYDELRHDAIVPGFRKGHAPRRLIEKRFGSEVRESL
                     TTSIVGQSYFAVTDKHELDVLGEPLFRIDAGEEGVKLMEFDEALQHMKLPAEGDFDYV
                     CEIEVKPTFELPELEGIQIKSPEIEITDQMVADEVLRQRKLRGRFEPQPEGGAERGDL
                     VIADVVLTVDDKQIKTEENVQLGVRPAALDGVQVPDLDKVLEGAKPG"
BASE COUNT         1201 a         2065 c         2095 g         1164 t
ORIGIN      
        1 atgccgagtt ggctgaacac gctgaacgac gggcggttga cgtgcgccgc gatcgccagc
       61 ccctcatgcc ggtgtatcag ccgcaccgcc gccgacaggt cgaagcccga agccatggcc
      121 agcattttcg gctcggaatc gacgatcgac ccgcccgaat caattacaag ctgctgcccg
      181 aacttctggt gatacgcgac ggtggcctcg ggcagagtag cacgcacctc tttcgcggcc
      241 gctgccgccg cggccgggct cggaaaaatg cccagaatgt gcacttcctc ggccgtcatg
      301 atttccatgc ccgccaggac cgccagtttg agaccggcga cggtgcggcc ggcacatagg
      361 ctggcggcag cagcggcctc ggcattgccg gccgcgttgt gatcgcaaat cgcgatcatc
      421 gacagctcgc gctccagggc cgcctgcacg atggccggcg gcgtcatctc atccgccgca
      481 cagggcgaca gggcggtatg gatatgcagg tcggcggccc agagcttcaa gggagcacct
      541 cgcgccgcga agcacgtgat agggtgacaa ggtgataacg tgatagagtg ataagggcgc
      601 ggcgcgggca gacttgcgtc gagtgctgaa tccttatcac cttatcacct tatcacttta
      661 tcactgtatc acttcgtatc actctgccgc ggccctcgca cgcccatttc ccacagtcgc
      721 ccgaccacct caaatgccga agccttcgag cagtacaggg cgatgccttc ttccttcgcc
      781 tttgcctgaa cagtgtcatc cggaacgcga tcagaagcga agataatggc ggccagttcg
      841 gcgtggaccg cgaccgcgat cacgttcagg tgcacctgaa ccgttaccag aacgccgccc
      901 ttcggtgcgt tcgccagtac atcgcttagc aggtccgaga cgtatccgcc ggtcacgtcg
      961 gccgcggccg ggctgatctc aggcgtcagg ttttgcagcc ccagtctttc cgcgatttcc
     1021 gcgagagtca tttactttcc tccgctggag gcccgggccg cgactgcttg gcggccggtt
     1081 cgttatccat cctctgccgc acgcaagcat cgagctgggc acgccccaga acaatgtcct
     1141 cggccagtgc cgcgcaggtc ggcgctccac aattgccgca attgctgccg gggaggctgc
     1201 ggcgcagttt atcaatcctg gccaacttct gaatggcgcg cgtcatgtcc gcatccagcc
     1261 gcagcccgtg ccgtggctcc agcggaacca cgcgtcgcac cgcccgcgcc cgcggatcgg
     1321 gtttggccgg cgccagggcc cagcgatatt gggccacgct ggcgacttcc gtaaacagcg
     1381 gcgatccaaa acagcccgca aaacaggcat acggctcgat taccgtgatg tcggtcagca
     1441 gcccgtcctc cagccgttcc aaaacctgcc gcactttatg gattccagtc acgcgcaaga
     1501 cgcccagcgg gtccggagcg gccggcgttg accccgtgtc ggcggagcgg cctgttcccc
     1561 gctttaggcg cgggtgcaac acggccgcga acgtcgccgg cagcaccgcc agaacgtggg
     1621 accgcttttc caccggtctg tcgcgggacc gcacaatcgt caactggcac gggcagggta
     1681 ccagcgacag cagcctgcga tcctcgccga ccacgctctc ataggcggcc tcttgggctg
     1741 aaagaaacgg ggccacgtgc ggcagcagcg acgggaagcg caactgaatc aggttcacaa
     1801 ccgccgggca cacgggtgag atcacagggc ggacgcgcgt ctcgccggcc gcgtattgca
     1861 tgacggcctt acgtagcgcg ctttcccagg ccgccgttac gcggatatcc gagaagccca
     1921 ggtacgccag ctcagttagg acttgccccg cagtaatacc cgccccgaac tgcaccaggc
     1981 tcaccggagg cagtatgagg atggaaccgg gggatggctt aagctcttcg cccgcggacc
     2041 gcagacccca cgcaccgctt gggcacgccc caatgcactc ggtgcagtca atgcacaagt
     2101 aatccaggat ctcaggcttg ccccgcagca cccgcagtgc ctgcgtcggg caagcccgca
     2161 ggcaatgcag cgatcggcgg catttttcgg cttcaacctg gatcgagaac tgccccgcgc
     2221 cgtacagcgc ctgcggacgt aggtgaatcg taaaactgac gcgagtgcca cgtccggccg
     2281 ccgattcgat cgcgaaatga tcgctgtttt tcttgatatt cggcaggccc atgcccgcgc
     2341 cgaagcccag ttcccgggcc ttggccgaag ccgtcgagaa gccgggcttc atggcctgtt
     2401 ccacgtcggg aatgccgggc ccggtgtcga tcacgtcgac gcgtaattcg tgctcgtcca
     2461 gttccgcccg cagttcgccc ccgtgcgagt gaattaccac attcatctcg gcttcgtagg
     2521 cggccaccat ggcccgcctg acaaccgccg gatccgcccc gattttctta agttgttctt
     2581 taatctggcg cgaggctgcc ccacccgttt cgtaattgcc cccctcgatc tggtacgaga
     2641 cggaggtcac ggcaaatggt cttcctccgc aaggcagcgg taaattcggc cgcaggtttc
     2701 gaagagcccg caggatgaaa ccaggatggc ggttccgtgt tcgcgggcgc tgcggagcat
     2761 cgtctcgtcg ggcatctggc tgttcaccag gcagatgcag ggcacgtcga ggagaccggc
     2821 catcatggtg agctgtccgc tggcgaggtt ggtcaccatc aacacgtgct cgctggccgc
     2881 attgagcaga tcgctgatgc gatcaccggc gtaaacctgg tcggcagcga catttagcgt
     2941 cttgccgggc gtcaggagcc gggctccgat tttctctgcc aaagtggcca gcttcattaa
     3001 gaactccagt ctcggttcgc gggtctgctc acggcagggg cttcgcgggt ctgctcacgg
     3061 caggaacctg gacattatcc gcccttatcg agcggataca agcaagcggt acgccaggat
     3121 gggtgaaaat ggggccggtc agccccttca ggatgacagc gcggaattgc gcgcggaatt
     3181 tcgcgcagta gcagagccgg ggcgttcgcc ccgcaatacc gccggccgtc ctcacctggg
     3241 cgacgcgccc ccgccctcag cgagcgccgc ccggtaggct tccagcgcgg ccggaaacgg
     3301 cccgagggct cgctcgaaaa tgcccagcga atacgcgatc gccaagccgt aattcgtgat
     3361 cggaacgccg gcgcggcggc atttcaagat tcgcgaaagc acctcgcgcc ggttctggac
     3421 gcaggcgccg cagtgaatca cgagcttgta ctcggccagg tcgggcggga agtcgtgccc
     3481 ctgagtcgta gtgaattcga gctcgccgcc cacgtactgc gttagccagc gcgggatctt
     3541 cacccggccg atgtcctcgc cgatcgggtg atgcgagcag gcctcggcga tgaggatggg
     3601 atcgccgggt cgcagccggt cgatggcggt cgcgccgcgc accatctccg tcaggtcgcc
     3661 cttgaagcgt gcgaagagga tagagaagga cgtcagcggc acctcgagcg gagtatccgc
     3721 cgagaccttc aggaacgcct gcgaatctgt caccaccagc gccggcgggc ggcgcagacg
     3781 gtcaatcgcc tggcgcagct ctcgttcctt cacaaccatg cagaaggctt cgtggtcgag
     3841 caggtcgcga atcgtctgca cctgcggcag gatcaggcgc cccttcggcg cttccttgtc
     3901 gatgggaacg acgagtagtg ccagctcgcc gggcggaacg aggtcgccga tgatcgccgg
     3961 cgtgttgaca aagtcgtccg gcgcggaacg gaccagggct tcgcgcaaat ctgccagtcc
     4021 atcaccgcgg ccggcgaccg tctggacgtg ccgaattttc cgttcgttca gccgcgccag
     4081 gacctcgggc gacggcgacg ccaaatcaca tttgttgaaa accgcaattg ccgggacatt
     4141 ccggccggcc agttcgttca gaatgccttc ttcgaaagcg ccccactcgc cggcctcggc
     4201 aacgatcagg ccgacgtcgg tgcggtcaaa aatccggcgc gtccgctcaa cacgctgctg
     4261 cccgagggcg ccgatgtcgt ccacgccggc cgtgtcgatg aacagcaccg gcccgatggg
     4321 cagcaattcc atcggctttt cgaccgggtc ggtagttgtg ccggcgcagg ccgagactat
     4381 cgacacttcc tgccgcgtga gcgcgttcaa caaggatgac ttgccgacgt tgcggcggcc
     4441 gaagatgccg atgtgcagcc tgagaccttt gggtgcgttt tgcattgaat ttcaccgcag
     4501 gggatacaga aggtacagag gaatagcggt tgtgtgcttc aggttcactc tagcttagcg
     4561 tcctttgcgc tcggctgtct cggcggttgc cttgaccggc ctatcgctgg ttgccgtatt
     4621 cacctttgct tgaggcgcgt aacggcctca ggggataata gctcgtcgga cggagcttcc
     4681 gtgagcaagc cgcattccac gacgatttcg cagacgccgc ggctcggggg agcggcggcc
     4741 gcaagctaaa gttcagcttc gccgatagga catgcggctc cgcctgacag gtgctgcgtc
     4801 ttggacagtg acgtgtctaa cctcgagtgc gacttctatg cgggcggccc gcctgagacg
     4861 gatgcggccg tgaatgccgg cgcggcggcg cgcccctaca tcggcgtcct gttcgattgc
     4921 tgcggcgtgt atgtgcgggt ttaccgccgc cccgagcaga acgagtacag cggccggtgc
     4981 ccgaattgcc tgcgccccgt caaagtccgc gtcgcacccg acggcaccaa ctcgcgcatt
     5041 tttcgcgcaa gataggaaga gcgtttcgaa gatgcgcagc gaagctgagt gccgcccggg
     5101 ctcatctcca gtgtgttgtg tcgcgtgagt tcggggccct tcttgacacc ccgggtgggg
     5161 ggcttaacgt acaaggcggg aagggccttg cgtgcccgcg cctggcgccg ctaaacgagt
     5221 ggcatgcccg cgctaagagc ttgggcatgg cacgcacacc gggtgccccg cgggcgtagg
     5281 tgtagaagag acggacagtc tgttgaaccg gggccagatg gcttcgccgc cggttctgat
     5341 gccgccaaga aggaaaccgg ttccgatggc gccgataagg agatctaacg tgcggtctga
     5401 catgcagcgc ttttggaagg tgttcgctcc gctgtcgtgc ggaggagtaa cgctgggctt
     5461 gcttgacgcg ttcggcatgt tctattggtc gaacatcttg gtggaagtgc tctcgtcgct
     5521 gtttatagct ctgttcggcg cctttttggg ccagcaggcc ggcgggctgt ttgcctaacc
     5581 ggccggcgca aaacggagag ttgggccgca aagcggagct ttgtacggcc cgccgcgaag
     5641 cggagcttcg cacgggcgaa taatctagaa acgaccccgg ctggcgggtg tccacgacgc
     5701 cgggccggtt accttattaa gggagatgga catgcagcta cccggcatgc cgggcatacc
     5761 cggcgcgatg gaagatccgg ttcttcgcga aaaggcggaa aaggaggcgg agcacctact
     5821 tgaggacctg aagaaggaag tcaaggcgga catcaaggac atcggcaccc tccgcaaaga
     5881 gctgcacatc acagtgccgg ccaaggtcat cgccgaccac ctcgaccaca actacgacga
     5941 gctgcgccac gacgcgatcg tgcccggctt ccgcaagggc cacgctccgc gacggctgat
     6001 cgaaaagcgc ttcgggagcg aggtgcgcga atcgctgacg acctccatcg tcggacagtc
     6061 ttacttcgcg gtcaccgata agcacgagct ggacgtgctg ggcgagccgc tgttccgcat
     6121 cgacgccggc gaagagggcg tcaagctcat ggagttcgac gaggccctgc agcacatgaa
     6181 gctgccggcc gagggcgatt tcgactacgt gtgcgagatc gaggtcaagc cgacgttcga
     6241 gttgccggag cttgagggca tccagatcaa gtcgccggag atcgagatca ccgatcagat
     6301 ggtcgcggac gaggtactgc ggcaacgcaa gctgcgcgga cggttcgagc cacagccgga
     6361 gggtggagcc gagcgcggcg acctggtcat agcggatgtc gttctgacgg tggacgacaa
     6421 gcagatcaag actgaagaga acgtgcagct tggcgtgcgt ccggcggcgt tggacggggt
     6481 gcaggtgccc gatctggaca aggtgttgga aggcgccaag ccggg
//
LOCUS       DAOSYS010000202         6509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10061_length_6509_cov_5.438178, whole genome shotgun sequence.
ACCESSION   DAOSYS010000202 DAOSYS010000000
VERSION     DAOSYS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10061_length_6509_cov_5.438178"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(896..1690)
                     /locus_tag="PLU99_11900"
     CDS             complement(896..1690)
                     /locus_tag="PLU99_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO17796.1"
                     /translation="MPTKTAKSAKARPGNKDVARIWRNYQRTRSTEHRNQLVEHYMPL
                     VHKLAEIIARRLWPRVTADELASAGYDGLIGAVTSFDPKRGVKFETFCRQRILGAIRD
                     WQREIDPLGRSGRNFERAVTAAEDHLQARTGRVPNSDELARHMGLPLSRFIKMKNTVL
                     ASHCVPLESGSDRSDDPRASALVPVDPGPSPVHHTERELIREHITRGLKEQDRLILTL
                     YYYEKLTMAAIGTVLGVSESRVCQRHAEIMEQLRARFVDSACDLVA"
     gene            complement(1992..3704)
                     /locus_tag="PLU99_11905"
     CDS             complement(1992..3704)
                     /locus_tag="PLU99_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOO17797.1"
                     /translation="MPTLIERIACKLAHLHAGRVYRQFLRAVDNTPHEQARALRRALR
                     VIDGSDFARRYQLTPDTAAADLRRMVPLCGYEALRPYIERVWAGEVRALFRARSPILM
                     FATSSGTTAKAKLIPVTREFVRDYRRGWNVFGLKMLTDHAAAVLRPILQVTSRYDGRR
                     SPVGIPCGAITGLLAKMQKRIVRRFYVGPPEVANIPDALARYYTLMRLGIARDVSFAI
                     TANPATLIQMARTAAQESEELIRDVADGSLSRRLVPDKALRDRLAAGLKPDRSRAAEL
                     ERLRAAYDTLRPRDYWKLSFLACWTGGSLEHYLDTLRSWYGDIPIRDIGLLASEGRVT
                     IPFEDGVPAGALDVTAAFFEFIPIECCDQEQPQTLLASELETGQDYAVVLTNTAGLVR
                     YRLDDVVRVHGWLGKAPVLEFLYRAGRVASVAGEKLTEHHCAAAVRNVCQAFKLPELD
                     FVLAPCWGDPPYYRLSCEVKLSAAQVEALDRELGVQNEEYASRRASMRLGAVQLRLLP
                     PGSLAAMDNKMMKARGAVAEQYKRVYLLTKPGDDDGILTTMRHAPRQNAGAFQSAVPA
                     RGGA"
     gene            complement(3718..4821)
                     /locus_tag="PLU99_11910"
     CDS             complement(3718..4821)
                     /locus_tag="PLU99_11910"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO17798.1"
                     /translation="MSLAWVITEVLTHNWALVAIVSTIITLIVVPLLIVLKYVRISLN
                     IMRTTKPPLARNPLDFERLTGEPVSFPAYDGMPLAGMILRGKAAGPRRGMIIFAHEFC
                     SDMFSCARYCSPLLALGYDVFTFDFRGHGRSACEPDYTPRQWVTDRELNDMRGAVAFI
                     QNWLEERGLPREVGIFGISRGACAAILASVENPEIRAIVADGAFSTDTTIEYFMKRWA
                     YIFARVRIVYENHPPVFWRFLRWAMMHFAAREFKCRFPSVRKAIQRMTPRPILFIHGE
                     KDSYLPVEYASRRLYALAGQPKYLWVAPGSRHNQAAVRHAEFYAWLTTSFFDRYLARI
                     PQAWQVADAPPIEATEAGAVEHDLAVDGTRSPS"
     gene            5047..>6509
                     /gene="pepD"
                     /locus_tag="PLU99_11915"
     CDS             5047..>6509
                     /gene="pepD"
                     /locus_tag="PLU99_11915"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOO17799.1"
                     /translation="MNMTNDTSSAVASLEPQAVWQYFSGIAAVPRASKHEERIREHVR
                     RLAQERGFNVREDSAGNLLIEVPATPGHENAPITVLQGHLDMVCEKNAGTAHDFDRDP
                     IRLILDRDPATGEEIVRADGTTLGADNGIGVALALAAATSPDVVHGPLEILCTVDEEQ
                     GMTGAAALQPDFFRGKRLLNLDSDEDTVIYIGCAGGGDITLSWEFPTRPVSGRLELAR
                     LTVSGLRGGHSGGDIHENRGNANKILVRTLLDPDVKRVQIASITGGSKRNAIPREANA
                     VIVASKRTLSALKAAAVRVQEAVRRESAEPNLNIAVEPVDRGAVPASLTVIDTQRLLW
                     ALEALPHGVLEMHQKLKGLVQTSNNVATIVSEPQPERNRMRIVVGCLSRSSMEGRVQT
                     TWSQIAAVGNLARATVESGNSYPGWNPNPESPLLGICQRVYRELFQSEPNVAAIHAGL
                     ECGIIGRQMGGDLDMVSFGAKIHGAHSPDERTYPASV"
BASE COUNT         1241 a         2035 c         2045 g         1188 t
ORIGIN      
        1 gaagcggttc atgccgggaa aagcgcgccg tatctgctcg cagaaatgca gcagggtttc
       61 gcgcgacggc gcgaggtcga gctttagata gagtcgcgtc gcgacgcaga actcatcgca
      121 aaacgttccg aagatccaca tcgcgccggt cctcgcgtcg cccctgtata agctcgtcca
      181 atctgatatt atcccaagcc gcctgttaac ggtaaacggc cctgtcaccc cgtcgcttcg
      241 aggagattac agaggcacgc gaggcattca gaatgccgca tgggctgatt tgcttggcca
      301 aacttatcat ggacgatttg cgcggctgcc gacgctatct ccatgctaac cctagactta
      361 gcggctaaga acgggctcac cgcagactac ttggcggacc aatcgcaggc agttggcctc
      421 aaaccgcgac gcggcatcgg cggcaccctt tgggctcgtt gccagacctg ccggggcggt
      481 gcggaccggc cgtcgcgtta cgtgccgaca tggatgcccc gccggtccag gaggctacca
      541 cctgccattc gccacccgca actccggcgt aatgcacccc tgcgggcatg acgcgccatg
      601 gccatgctgc tcgggcggcc cgcctgctta aaaattgtca agcgggactg ccgcacccga
      661 tccgtctcat ttttcagccc gcagacgaag acggaggcgg tgtggaggcc atgatcgcgg
      721 gtggcgccct ggacggcgtc gcgtgcgttt tcgggattta cgtgttgccc cagcatcccc
      781 gcggtttgac atcaacgagg aagctttgcc gctgggggct gcgctgctag cggaggtcca
      841 tcgaccgcgc aaattcaatg agggaagtcg gttacggagg tcgaccctga gccgtttaag
      901 ccaccaggtc gcaggccgaa tcgacgaacc gggcgcgcaa ttgctccatt atctctgcgt
      961 ggcgctggca gacgcgcgat tcgctcacgc ccaggacggt tccgatggcg gccatcgtca
     1021 gcttctcgta gtagtaaagc gtcagaatca ggcggtcctg ctccttcagg ccgcgcgtga
     1081 tatgctcgcg aatcagctcg cgttcggtgt ggtgtaccgg gctcgggcct ggatcgaccg
     1141 ggaccaatgc gctggcgcgc gggtcgtcac tgcgatcgct gcccgactca agcgggacgc
     1201 aatgtgaggc tagaaccgtg ttcttcattt tgatgaagcg gctcagcggc aggcccatat
     1261 ggcgtgccaa ctcgtccgaa ttcgggacgc ggccggtgcg cgcctgcaaa tgatcctcgg
     1321 cagccgtcac ggcccgttcg aagtttcggc cggagcgtcc gagggggtcg atttcacgtt
     1381 gccagtcgcg gatggcgcca agaatgcgtt ggcggcagaa ggtttcgaat ttgacgccgc
     1441 gtttgggatc gaaggaggtc accgcgccaa tcaggccgtc gtacccagcg ctggcgagct
     1501 cgtcggcggt gactcgcggc cagagccggc gggcgatgat ctcggctaat ttgtgcacca
     1561 ggggcatgta gtgctcgacg agttgattgc gatgctccgt cgagcgcgtg cgctggtaat
     1621 ttcgccagat tcgggcgaca tccttgttgc cgggcctggc tttagccgat ttcgcggttt
     1681 tggtgggcat cttcgccact ctccttactc gttttcttct cgcgagggct gacgaaaatc
     1741 cggcgatccc gcgagacacc cttatgaact taagtaggac gcggatgctg taattatcga
     1801 actgagagga gcagatactt taacaaatta ccaggaaaag cggttttgaa ctttaactat
     1861 gttgacttct atcggagtct gagtgcgaag acagcaaaaa cacacctttt ccacaaggtt
     1921 atcagacgcg caacgaacag ccgcgctatc tggtcgtcgc cggcaaagtg ttgatctgtt
     1981 gaatgttgcc cttatgcgcc gccgcgagcc ggaaccgcag attgaaacgc gccggcattc
     2041 tggcgcggcg cgtgacgcat agtcgtcagt atgccgtcgt catcccctgg cttagtgagc
     2101 aggtaaacgc gcttgtactg ttccgccact gccccgcgag ccttcatcat tttattgtcc
     2161 attgccgcca ggctgccggg cgggaggaga cgcagttgca cggcgcccag acgcatggac
     2221 gcgcgtcgtg atgcatactc ctcgttttga acacccagtt cacggtccaa cgcctcgact
     2281 tgcgccgcgc tgagcttaac ctcgcagctc agccggtaat aaggtgggtc gccccaacaa
     2341 ggcgccaaga cgaaatcaag ttcgggcaac ttgaaggctt ggcagacgtt gcgaactgcg
     2401 gcggcacagt ggtgctcggt aagtttttcg ccggccactg aggccacccg cccggcgcga
     2461 tataggaatt cgagcaccgg cgcttttccg agccaaccgt ggacgcgtac cacgtcatcc
     2521 agccggtaac gcaccaaacc cgccgtgttg gtcaacacga ccgcgtaatc ctggcctgtc
     2581 tcaagctcgc tcgcgagaag cgtctgcggc tgttcttggt cacagcattc gattgggatg
     2641 aactcgaaga acgcggcggt cacgtcgagc gcgcccgctg gaacgccgtc ctcaaacgga
     2701 atggtaacac ggccctcact ggccagcagc ccgatgtcgc ggatcgggat gtccccgtac
     2761 cagcttctga gcgtgtccag gtaatgctcc aggctgccgc cagtccagca tgcaaggaaa
     2821 cttagcttcc agtagtcgcg cggccggagt gtgtcgtaag cggcgcgcaa gcgctcgagt
     2881 tccgcggccc tggagcggtc gggtttcagg ccggccgcaa gacgatcgcg gagggcctta
     2941 tcgggcacga gccggcgtga caggctcccg tcggcgacgt ctctgatgag ctcctcgctt
     3001 tcctgcgcgg cagtccgcgc catctgtatg agagtggccg gattggcagt gatcgcaaag
     3061 ctgacgtcgc gggcaattcc caggcgcatc aaggtgtaat agcgcgccag tgcgtcaggt
     3121 atgttcgcca cctccggcgg accgacgtag aaccggcgga cgattcgctt ctgcatcttc
     3181 gccagcaacc ccgtgatcgc gccgcacgga atgcctacgg ggctgcgccg gccgtcgtag
     3241 cggctggtaa cttggaggat gggtcgcagg actgcggccg catgatctgt cagcatcttc
     3301 aggccgaaga cgttccaacc gcggcgatag tcgcgtacga attccctggt tacaggtatg
     3361 agcttggcct tggcggtcgt gccgctgctg gtggcgaaca tgagaattgg tgaacgggcc
     3421 cggaagagcg cgcgaacctc gccggcccaa acgcgttcga tgtacgggcg gagggcctcg
     3481 tagccgcaca atggaaccat acggcgcagg tcggcggccg cggtgtccgg cgtcagttga
     3541 tagcggcgtg cgaagtcgct gccgtcgatt acccgtagag cgcggcgcaa ggcacgggct
     3601 tgctcatgcg gcgtattgtc gaccgctctt agaaattggc gatacactcg cccggcgtgc
     3661 aggtgggcga gcttgcaggc tattcgctcg attaacgtgg gcattcgctg gccccgttca
     3721 acttggcgag cgggttccgt cgacggccag gtcatgctcg acagcgccgg cttcggtggc
     3781 ttcgattggg ggcgcgtcgg cgacctgcca agcctgcgga atgcgggcca ggtagcggtc
     3841 aaagaaagaa gtcgtcagcc aggcatagaa ctcggcgtgc cggacggcgg cctggttgtg
     3901 gcgcgaaccc ggcgcgaccc acaggtattt cggttgaccg gccagtgcgt agaggcgacg
     3961 actggcgtat tcgacgggca aatacgagtc tttctcgccg tgaatgaaaa ggattgggcg
     4021 cggcgtcatg cgctggatgg ccttacggac tgagggaaag cggcatttaa actcgcgggc
     4081 cgcaaagtgc atcatggccc agcgcagaaa acgccaaaac acgggcgggt ggttctcata
     4141 cacaatgcgc acgcgtgcga aaatgtacgc ccagcgcttc atgaaatatt cgatggtcgt
     4201 gtcggtgctg aacgcgccgt cggccacgat ggcgcgaatc tccgggttct cgactgacgc
     4261 caggatggcg gcgcacgcac cgcgcgagat gccgaaaatg cccacctccc tgggcaggcc
     4321 gcgttcctca agccagttct ggatgaatgc aacggcgcca cgcatgtcgt tcaattcgcg
     4381 atccgtgacc cattggcgcg gcgtgtaatc gggctcgcaa gcggaccgcc cgtggccgcg
     4441 gaaatcgaac gtgaacacgt cgtagcccag ggccagcagc gggctgcaat aacgcgcgca
     4501 ggaaaacatg tccgagcaga actcgtgggc gaagatgatc attccccggc gcggcccggc
     4561 cgccttgccg cgcaggatca tcccggccag cggcatgccg tcgtaggcgg ggaagctgac
     4621 cggttcgccg gtcagtcgct caaaatcgag cgggttgcgc gccaacgggg gcttggtagt
     4681 ccgcatgata ttgagcgaaa tgcggacata tttcagcaca atcaggaggg ggacaacaat
     4741 tagggtaata atcgtcgaga caatggccac cagggcccag ttgtgtgtaa ggacctcggt
     4801 gattacccac gccaaggaca ttgcaaatcc gtgtcgggcc ggcatgcaga ccgcatcgtt
     4861 tcgcgggatg gtagcgtaag gggcgtttaa agtccatgcc ggcattcggt tcgataatcc
     4921 gccggcgggc ggcgcgccgc acccgtgccg cggccggctc tgcacttgcc ttctaccttc
     4981 tgccgcccat gacgctgcgc gccggttggg ttaatatgcc ctctttcgtt ggacccggat
     5041 gaggaaatga acatgactaa cgacaccagc tccgccgtgg caagcctcga accgcaggcc
     5101 gtttggcagt atttcagcgg catcgccgcc gtcccgcgcg cctccaagca cgaagagcgc
     5161 atccgcgagc acgtccgccg cctggcccag gaacgcggct ttaacgtccg cgaggactcc
     5221 gccggcaatt tgctgatcga agtgcccgcc accccgggcc acgaaaacgc gccaatcaca
     5281 gtgctccaag ggcacctcga catggtctgc gaaaagaacg ccggcaccgc ccacgacttc
     5341 gaccgcgatc cgatccgcct gattctcgac cgcgatccgg ccaccggcga agaaatcgtc
     5401 cgcgccgatg gcaccacgct cggcgcagat aacggcatcg gcgtcgccct ggcgctagcc
     5461 gccgcaacct cgcccgacgt ggtccacggc ccgctcgaaa tcctctgtac agtcgacgag
     5521 gaacagggca tgaccggggc ggccgcgctt cagcccgact tcttccgcgg caagcgcttg
     5581 cttaatctcg actccgacga ggatacggtc atctacatcg gttgcgccgg cggcggcgac
     5641 atcaccctga gttgggagtt tcccacgcgg cccgtgtcgg gacgcctcga actggcgcgc
     5701 ctcaccgtca gcggcctgcg tggcgggcat tccggcggcg acatccatga gaaccgaggc
     5761 aacgccaaca agatcctcgt ccgcaccctg ctagaccccg acgtaaaacg cgtgcagatt
     5821 gcgtcaatca ccggcggcag caagcgcaac gccattccgc gcgaagcgaa cgccgtgatc
     5881 gtcgcctcaa agcgtacgct atccgccttg aaagcagcgg cggtcagagt ccaagaagcc
     5941 gtccggcgtg aatccgccga accgaacctc aacatcgccg tcgagcccgt cgaccgcggc
     6001 gccgttcccg cctcgctcac ggttatcgac acgcagcgtc tgctttgggc gctggaagcc
     6061 ttgccgcatg gcgtgctcga gatgcatcag aagctcaagg gcctggttca gacttcgaat
     6121 aacgtcgcca ccatcgtcag cgagcctcag cccgagcgca accgcatgcg catcgtcgtc
     6181 ggctgcctca gccgcagctc gatggaaggc cgcgtgcaga ccacctggtc ccagatcgca
     6241 gccgtcggca acctcgcccg agcgacggtc gagagcggca acagctaccc cggctggaat
     6301 ccgaatcccg aatcgccgct gctgggcatt tgccagcgcg tttaccgcga gctgttccag
     6361 tcggagccga acgtggccgc cattcatgcc ggcctggaat gcgggatcat cggcagacaa
     6421 atgggcggcg acctggacat ggtctctttc ggcgcgaaga tccacggcgc gcacagcccg
     6481 gacgagcgga cttatccggc ctcggtaca
//
LOCUS       DAOSYS010000203         6505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10067_length_6505_cov_5.083101, whole genome shotgun sequence.
ACCESSION   DAOSYS010000203 DAOSYS010000000
VERSION     DAOSYS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10067_length_6505_cov_5.083101"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLU99_11920"
     CDS             complement(<1..466)
                     /locus_tag="PLU99_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17800.1"
                     /translation="MPEQARILRGVVVLLVLLVVATAAAQNEVLRKVSELFQQQEYLA
                     AQAELSKVDRDQLDQADQEEYDRLSEQVAAGVAGFERADRDLVKAGVAFDEGRWDAAD
                     ALYQAVLENEFARAEQKSRAAEQRKRIDERRKLAEAAAPGIVEVRPAEPPAQP"
     gene            complement(1014..1247)
                     /locus_tag="PLU99_11925"
     CDS             complement(1014..1247)
                     /locus_tag="PLU99_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17801.1"
                     /translation="MNRCETCGNEYDKTFEVLKDGRRHTFDSFECAVQALAPTCEHCG
                     CRVIGHGVEAHDRIFCCANCAQAAGEKALQDRA"
     gene            complement(1367..1561)
                     /locus_tag="PLU99_11930"
     CDS             complement(1367..1561)
                     /locus_tag="PLU99_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17802.1"
                     /translation="MPRRMSPESVRRRMESDDRPLLVCGYDNDRKCQENAIEGAITYN
                     QFVNQLDQIPKEREIIFYCA"
     gene            complement(1722..2738)
                     /locus_tag="PLU99_11935"
     CDS             complement(1722..2738)
                     /locus_tag="PLU99_11935"
                     /inference="COORDINATES: protein motif:HMM:NF018880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOO17803.1"
                     /translation="MKWKHCRGAVVAGAVLASLLLGAIGGCGQAGRCEAETSSPELVA
                     GEQVSPVQGSAAAYVGSGDPYTPGPLSTRKIELDACDFGSPQPLLIFAPVTPAAYPVV
                     VFQHGFLGKNSDYSELLRHLAGHGFVLVAPQMYSGLGVVLGDPRPPEEARRAALVLDW
                     LPAGLPAALGYTPDVRRLGLAGHSRGGQVCWLLLAAHPSRVLAAAAIDPVDGAEPRGN
                     RTWAAEEPLSFSGPALVIGTGLGGRCAPAGQNHEQFYAVCRSPAWHVFVPHHGHTDML
                     NEGPAMLGRLMCDGGPDRAGMRRLTAGLLVALFRGALQGDESAYAYLSDASSAVLEVV
                     VERR"
     gene            complement(2814..4040)
                     /locus_tag="PLU99_11940"
     CDS             complement(2814..4040)
                     /locus_tag="PLU99_11940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOO17804.1"
                     /translation="MARSAAGPPGAQSPWVQLRSATLHPFIFQRMVRAADPAARPGDV
                     VTVYDKAGVLFGHGLFNPQSQIAIRMLAYGETPIDEGFWRARLAQAVGLRRQLGLDQV
                     TDAYRVVHSEGDNLSGLIVERYADCLVFEIFALGMFQRWGEFAALLADELGPPTSLDR
                     PYKAGPAWRTFVRADAQIEAIEGFQVAAAQQDAPARLVIREHGVRYRVDIVGGHKTGF
                     FCDQRDNRLRLARLCGGADVLDVCCYTGGFALCAKVAGGAASVTGVDLDEAAIELARE
                     NANLNQVRVDLVHADAFAYLRQMQANGRQFDVVVVDPPKLVTSRRDLEEGLAKHHDLN
                     ALAVQLVRPGGIMLTCSCSGLVSPDAFLGAIHAAGRRTGRALQMFDYTGAAPDHPIML
                     NCPESGYLKAVWLRVM"
     gene            complement(4617..6464)
                     /locus_tag="PLU99_11945"
     CDS             complement(4617..6464)
                     /locus_tag="PLU99_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOO17805.1"
                     /translation="MGVRFCLGLLVLSLCGCRVGPDYRRPQMPVPDEWSGMQAGAVAT
                     RPAGPEIPEHWFASAQPASPTSQPGAPALESSVPISEPAVVAWWAGLQDPVLDGLIAR
                     AAEANLNLRIATARVLQARAQRDIAIAAYWPQVNFSDLYDYRGSSLNAGPDIKAPRGP
                     LTGLPTIDVVPGAPPIVQVVPARIGPLSSGGGRSPSVSIQPGIFNTAGGSTPPFAVLS
                     PPSLTPAAEVPRQQNLFQAGFDAAWEIDVFGGIYRSVEAATAALEAAEWDRRDVLITL
                     LSDVALNYVQLRGSQRRLHIAYQNIVTQQEAVRVTRARYEAGFTGELDVAQAQAQLST
                     TESQLPVLQTAIQQSIYQLSVLLALPPGALLEELRMAEPIPAAPPVVPVGLPSDLLRR
                     RPDVRSAERQLASATAQVGVAVAELFPVFALTGSFGTATSDFRHILDSGSLFWSVGPS
                     LAWPIFQGGRLRANVRLQDAIAEQALANYELVILRALQEVDSSLVAYLNEKVRYDSLA
                     EAVVANQRATELSNELYARGLTPFLNVLESQRALFISQDAQAQSQTLAILNLVSLYKA
                     LGGGWEPSEPCVIEFRGVSGLKSYAELNALEEADCDLDAIRGSEELESE"
BASE COUNT         1202 a         2411 c         1798 g         1094 t
ORIGIN      
        1 ccggctgcgc cggtggttcc gccgggcgga cttccacgat gcccggggcc gccgcttccg
       61 ccagcttccg ccgttcatcg atccgcttgc gctgctcggc cgcccggctc ttctgttcag
      121 cacgcgcgaa ctcgttttcg agcaccgctt ggtacaaggc atccgcggca tcccaacgac
      181 cctcgtcgaa tgccacgccg gccttcacca ggtctcgatc ggcgcgctcg aaaccggcca
      241 cgcccgctgc gacctgctct gaaagacggt catactcctc ctggtccgcc tgatccaact
      301 ggtcgcgatc gaccttgctc agctccgctt gggccgcaag gtactcctgc tgttggaaca
      361 gctcacttac tttgcggagc acttcattct gcgccgcggc ggtcgcaacc acgagcagaa
      421 ccaatagcac cacaactccc cgtaagatcc gcgcttgctc tggcatggaa tctccttcgt
      481 tcccggcaac cgggctgcca tgaatcaccc ggcctgccgg ccgttgcagt gcgcgcctcc
      541 acaccgcacg gccatccaac ttctcaggcc acatcaacgg ctggatggat gtatgaagcc
      601 atcgaggcgc ccatcataaa actgggcggc agtgtagtcg cgcaattaca gacacgcaat
      661 acggattttc cggctaacag agacgaaaaa ttagtcggac gtgttatcca gacgtgtcac
      721 cgttggcgat tatccgccac ggccacggca tgcccgcggc tacgcctgat aacatgctgt
      781 tcgccaagcc ccaccgctcg ccaggcttgc gcgggccccc ggccaaatac gacgcgctcg
      841 ccaagatcac ggtcacaacc gacggcggcc caccccttct cccccttctc acgttccccc
      901 cttctcacgt tcgcgccctt cttacccttt ggagcgtcgc atgttgaaac ccggaccacg
      961 gcgacgaatg tgccgcggtc cgggctccag tcccgcccca agatgccctt cactcaagcg
     1021 cggtcttgca gcgctttctc gcccgccgcc tgagcgcagt tcgcgcaaca gaaaatgcga
     1081 tcgtgcgctt cgaccccatg gccgatcacg cggcagccgc agtgctcgca ggtcggcgcc
     1141 agcgcctgca ccgcgcattc aaagctgtca aacgtatgcc ggcgcccatc tttgagcacc
     1201 tcgaatgtct tgtcgtactc gttcccgcac gtctcgcaac ggttcatggg tgtgctcccg
     1261 ccgtcattct gcgccacgcc cccacgccac catcaaggat cgacgcgttt atgtatccct
     1321 tgcggaagta ttcatctgcc cgaccggcag ccgtcgcgtc gttcggtcag gcgcaataga
     1381 aaatgatctc acgttccttt ggaatctgat cgagttggtt gacgaactgg ttgtacgtga
     1441 tcgccccctc gatcgcgttc tcctggcact ttcgatcatt gtcatacccg caaaccagca
     1501 gcggccggtc atcgctctcc atgcgccgcc gcacggattc gggactcatt cgtcttggca
     1561 tcagcggtct ccttgacaca gccccgacgc ggcggcctgt cgcaaccaag cccccgttcg
     1621 gaacgccctc actttcctcc caccggctta acacgcgatg aatcacccat gaattgcgat
     1681 taaccccccc acgctttatc actctatcgc tccgtgtgaa ttcatcgccg ctcaaccaca
     1741 acctccaaca ccgcgctgct cgcgtctgac aaataggcgt acgcgctctc gtccccctgc
     1801 aacgcacccc gaaataacgc aaccagcaat cccgccgtga gacgccgcat ccccgcccgg
     1861 tccggaccgc catcgcacat gagcctcccc agcatcgccg gcccctcgtt cagcatgtcc
     1921 gtgtgcccgt gatgcggaac aaacacatgc cacgccggcg aacgacaaac cgcgtagaac
     1981 tgctcgtgat tctgaccggc cggcgcgcag cgaccgccca gccccgtgcc gatcaccagc
     2041 gccggcccgc tgaaactgag cggctcctcg gcggcccacg tccggttgcc gcgcggctcg
     2101 gctccgtcca ccgggtcaat cgctgccgcc gccagtactc gcgatggatg ggccgccagc
     2161 aagagccagc agacctgccc accacgcgaa tgccccgcca ggcccaaccg tcgtacgtca
     2221 ggcgtatagc ccagcgccgc cggcagtcca gccggcagcc agtccaacac tagcgccgcg
     2281 cgccgcgcct cttccggagg tcttgggtcg cccaacacca cccccaggcc ggaatacatc
     2341 tgcggcgcta ccaagacaaa tccgtgcccc gccaagtgcc gcaataattc actgtaatcg
     2401 ctattcttcc ccagaaaccc gtgctgaaac accacgaccg ggtaggccgc cggcgtcacg
     2461 ggcgcgaaga tcagcaacgg ttgcgggctg ccaaaatcgc acgcgtcgag ctcaatcttg
     2521 cgtgtcgaaa gcggacccgg agtatacgga tcgccggacc ctacatatgc cgccgcggac
     2581 ccttgcaccg gcgacacctg ctcccctgca accagttccg gcgaagatgt ctccgcttca
     2641 cagcgcccgg cttgcccgca cccgccaatc gcgcccaaca gcaggctcgc cagcaccgcg
     2701 cccgccacaa ccgcgccccg acagtgcttc catttcatgc tcgattgtat gagccgcagc
     2761 gcgtgccacg ggctaacctc gtacttagcc gcggcgccgt ttgcggcgtt tggctacatc
     2821 acccgcagcc acaccgcctt cagatagcct gattccggac agttcagcat gatcgggtga
     2881 tccggcgccg ctcccgtgta gtcaaacatc tgtaacgccc gccccgtgcg gcgccccgcc
     2941 gcatggatgg cacctaagaa cgcgtcaggc gacaccagcc cgctgcaact gcacgtcagc
     3001 ataatcccgc cggggcgaac caactgcacc gccaacgcat tcaggtcgtg atgcttggcc
     3061 agcccctcct ccaggtcccg ccgcgacgtg accaacttcg gcggatcgac caccacaaca
     3121 tcgaactgcc gcccattggc ctgcatctgc cgcaggtatg caaacgcgtc cgcatgcact
     3181 aggtcaaccc gcacttggtt caggttggcg ttctcgcgcg ccagctcaat cgcggcctca
     3241 tccaggtcaa cgcctgtgac ggacgccgca ccgccagcca ccttcgcgca cagcgcgaac
     3301 ccgcccgtgt agcagcacac gtccagcacg tccgccccgc cgcacagccg cgccagccgc
     3361 agccggttgt cgcgctggtc gcagaaaaac ccggtcttgt gtccgccgac gatatcgacg
     3421 cgatagcgca ctccatgctc gcggatcact agccgggccg gcgcgtcctg ttgagccgcg
     3481 gccacctgga acccctcgat ggcctcgatc tgcgcatcgg cgcgaacgaa cgtccgccat
     3541 gccggcccag ccttgtatgg gcgatcgagg ctggtcggcg gccccagttc gtccgccagc
     3601 agggccgcaa attcgcccca acgctggaac atgcccagcg cgaatatctc gaagaccagg
     3661 caatcggcat agcgttcgac gatcagcccg ctcaggttgt cgccctcgga gtgaaccacg
     3721 cggtaggcgt cggtgacctg atccagtcca agctgccggc gcaggccgac cgcctgcgcg
     3781 agccgggccc gccagaagcc ctcgtcgatg ggcgtctcgc cgtaagccag catgcggatg
     3841 gcgatttgcg actgcgggtt gaacagcccg tgcccgaata gcaccccggc cttgtcgtac
     3901 acggtgacga cgtcgcccgg tcgagcggcg ggatcggccg cccggaccat gcgctggaag
     3961 ataaacgggt gcaaagtcgc cgagcggagc tgcacccacg gcgattgcgc cccgggcgga
     4021 ccggccgcgc tgcgtgccac gcgtgttcgg ggccggccgg gactgaaacg atgtttcgag
     4081 ccgcgcttct ccatgcagga aatcattgcg cggcgtgccc caaccggcaa cccggagcag
     4141 agccggggcg tatcgccccg gatcaagagg tgccggcctg ctcccaaaat ccccgtcgca
     4201 cgctgctttg aaaatccccg tcgctcgctg gtgcgtgaat cctttctcgc accccgtctg
     4261 tgggaatccc ctcccacctc ccacgcagtt ttccctccgc gctcacccgg tcgcgcagcg
     4321 cgcggcaagc cgccccattt cacacgctgc gctgttcacc tcggcttgcc tgcaaccgcc
     4381 ctactccttt ttgtctttat gccccgcccc cagtgcagtc cgcgctatcg tcagcgcttt
     4441 cagcgcatat gcctgactcc ggtccgaccc gatgctgcaa cggcgacact gcgccacgga
     4501 gaacggggtg ccgaacaatc gtcgccgcta tgtcagataa cagtaacgcg gttcccctta
     4561 gaaagccagc cgaattgctc gcccaggtcg tcgtcgcccg cagtgggaca ccgtcgtcat
     4621 tcgctctcca gctcttccga accgcgaatc gcatccaaat cgcaatccgc ctcctccaac
     4681 gcattcaact ccgcatacga cttgagaccg ctgaccccgc ggaactctat aacacacggc
     4741 tcggacggct cccacccgcc gcccagggcc ttgtagagcg agaccaaatt caatatcgcc
     4801 agcgtctggc tctgcgcctg cgcgtcctgt gatatgaata acgcgcgctg cgattccagc
     4861 acgttcaaaa acggcgtcag cccgcgggca tacaactcgt tgctcaactc ggtcgcccgc
     4921 tgattggcca cgacggcctc agccagtgag tcgtagcgca ccttctcgtt caggtacgcc
     4981 accagcgaac tatccacctc ctgcaacgcc cgcagaatca ccagctcata attggccaga
     5041 gcctgctcgg caatcgcatc ctgcaaacgc acattggcgc gcagccgccc gccctggaag
     5101 atcggccagg ccaggctcgg cccaaccgac cagaacaggc tgcccgaatc caagatgtgc
     5161 cggaagtccg aggtcgccgt cccaaatgag ccggtcaggg cgaacactgg gaacagctcc
     5221 gccaccgcca cgcccacctg cgccgtcgcc gaggccaact gccgctccgc cgaacgcacg
     5281 tccgggcggc gccgcaacaa gtcggaaggc agtccgaccg gcacgaccgg cggcgcggcg
     5341 ggaataggct cggccatccg caattcttcg agcagcgctc ccggcggcag cgccaaaagt
     5401 acactgagct gataaatgga ctgctgtatg gcggtctgca gtacgggaag ctgcgattca
     5461 gtcgtggaga gctgcgcctg ggcctgcgcc acgtccagtt cgccggtgaa ccccgcttcg
     5521 taccgggccc gcgtcacccg gacggcctcc tgttgagtga caatgttctg atatgcaatg
     5581 tgcaggcggc gctgcgaccc gcgcaactgc acatagttca gagccacgtc cgaaagcagc
     5641 gtaatcaaaa catcgcgccg atcccattca gcagcttcca aagccgcagt ggccgcctcc
     5701 accgaacggt aaatgccgcc gaacacatcg atttcccacg ccgcatcgaa gccggcttga
     5761 aacagattct gctgccgcgg gacttcggcg gccggcgtga gcgacggcgg actcaggacg
     5821 gcaaagggcg gcgtagagcc gccggccgtg ttgaagattc ccggctggat tgacacactg
     5881 ggtgaacggc cgccgccaga cgacagcggt ccgatgcgcg ccggtaccac ctgaacgatg
     5941 ggcggcgcgc ccggaacgac gtcgatcgtg ggcaacccag tcagcggccc gcggggcgct
     6001 tttatgtcag gaccggcatt caggctggac cccctgtagt cgtagagatc gctgaaattc
     6061 acttgcggcc aatacgccgc tatcgcaatg tcgcgctgcg ctcgggcttg cagcacgcgg
     6121 gccgtggcta tgcgcagatt caagttggct tcggccgcgc gcgcaatcag tccatccagc
     6181 accggatctt gcagcccggc ccaccatgcc acgaccgccg gttcactaat cggaacgctg
     6241 ctctccaaag ccggagcgcc cggttgcgaa gttgggctgg ccggctgcgc gctggcgaac
     6301 caatgctcgg ggatttccgg cccggccggc cgagtcgcaa ctgcgccggc ctgcatgccg
     6361 ctccactcat ccggcaccgg catctgcggc ctgcgataat ccgggccgac gcgacacccg
     6421 cacaaactta acacaagcag gccgaggcag aagcgtacgc ccattccaca cctccggaag
     6481 ccgtgaccgc gctctgtggg attcc
//
LOCUS       DAOSYS010000204         6429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10227_length_6429_cov_5.451051, whole genome shotgun sequence.
ACCESSION   DAOSYS010000204 DAOSYS010000000
VERSION     DAOSYS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10227_length_6429_cov_5.451051"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLU99_11950"
     CDS             <1..231
                     /locus_tag="PLU99_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17806.1"
                     /translation="NGAVMPDSTLVVPGATDLTVIAVDSAGTGYIADRDTANAVFIYD
                     NIATRNGTIAPDRTLQGANTLLQRPISVFISE"
     gene            complement(328..744)
                     /locus_tag="PLU99_11955"
     CDS             complement(328..744)
                     /locus_tag="PLU99_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOO17807.1"
                     /translation="MTYSGRFTVATTGDSQVLDITSQVAEIVAESAVQQGLLVAFVVG
                     STAAITTTEAEPGLLTHDLKAFWERVAPRDAYYKHEETWHDDNGHAHVRASALGPSLA
                     LPVIDGRMPLGTWQQIVLLDFDTRPRRREVIVQIVG"
     gene            complement(839..2527)
                     /locus_tag="PLU99_11960"
     CDS             complement(839..2527)
                     /locus_tag="PLU99_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO17808.1"
                     /translation="MQPEVSVADVPGPAAQSRAPRPLKPLTDRLLIIGLDGATFYVLD
                     PLMNAGRMPNLKRFIEQGTAGVLHSTQPPITPAAWTTFMTGKGPGQHGILDFEKYDVF
                     QHQLTFNSTYQIEEKTIWELLSEKGLRVGSINVPMTFPPKPINGFMISGFETPSIDAE
                     FTYPRELKQEIFQQIPNYNYRTNWQRRALGGQDVLETNLEYIANSFEQGYRLTTYCGD
                     KYGWDVLMTVMKLVDNLQHKAWKYLDPQTAGNFPREAELAARVFTRLDDVLGRMFEYA
                     ERNNATVLIMSDHGHGSLDGKAQPNLLLKRWGYLALRSPWQQANTRAAYWWHRFTKGK
                     ATRFEQGSRGIERELAVDWTRTRACVMHAGIYGYLYINLKGRGPHGIVEPADYEPLRN
                     ELIERLRSASVRDRNGREVRIFPHVYKTETLYHCDRGENPNLPDLLLAPNPGLAVIRK
                     IRGGQPVRWCKSARLEGTHRDEGILALGGANVRHGQRTEASIIDIAPTMLAALGLRVP
                     VDMEGRVIREAFTTDPVIEREPPLKKAVEEAVEVYSDSDRRILEERLTELGYLE"
     gene            2747..3373
                     /locus_tag="PLU99_11965"
     CDS             2747..3373
                     /locus_tag="PLU99_11965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO17809.1"
                     /translation="MSEMPRWLAETPVDFAAIDSAPRDALRARLDIAGEQTAILVLPP
                     LTRAAGAEYAVWAALLLEKVRPDVRLVVPGPAEENRRIRYLVRSCRHQYMMRLAEELS
                     LAELVAAADIATFLPIAAVPLTGLAWAMAAGRTIVASEVPPMAALLEAGRTAWLCRPR
                     DPQDGARYMLKALDEPVVSARLAAAAREKALALFSAPRMVKPTNCNGV"
     gene            complement(3351..4268)
                     /locus_tag="PLU99_11970"
     CDS             complement(3351..4268)
                     /locus_tag="PLU99_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HOO17810.1"
                     /translation="MQSDFDVVTGAFGYTGKYIARRLLAQGRRLLTLTNHPPPENPFG
                     DQVTIAPLDFSHPEKLAASMAGAHTLYNTYWIRFPRGPLTFEQAVENSRILLTSAKEA
                     GVQRIVHISITNPSSQSPLPYFRGKGLLEEFLAGLGVPPAIVRPTVIFGNEDILINNI
                     AWLLRHVPLFAIPGDGKYRIQPVCVEDVAEIAVSAAQGTADVTVDAVGPEIFTFEELV
                     RLIAGKVGARAHITHAPPSIALLAAEMSGLALGDVMLTRDELHGLMANLLVSGQPPTG
                     KTRLSDWLDQHSADLGRHYASELKRHYSS"
     gene            4724..4882
                     /locus_tag="PLU99_11975"
     CDS             4724..4882
                     /locus_tag="PLU99_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17811.1"
                     /translation="MRFRLHTAQEQPQFGLVARVAPTCSVTTTSIPTNRRDTNWEVES
                     HMATVLQR"
     gene            4999..5448
                     /gene="mscL"
                     /locus_tag="PLU99_11980"
     CDS             4999..5448
                     /gene="mscL"
                     /locus_tag="PLU99_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOO17812.1"
                     /translation="MVRNLWQDFKDFAFKGNMLDLAVAVVIGTAFGAVVNSLVTNIIM
                     PAISYVQPSGEGYRAWQWGRIEIGAFLGQLVTFLIIAATVFLLIVKIVGTLIKRVSTP
                     PASPEPVTKECPFCLMTIPSKAIRCGHCTADLSPAAGAKSAPGLMPG"
     gene            complement(5599..>6429)
                     /locus_tag="PLU99_11985"
     CDS             complement(5599..>6429)
                     /locus_tag="PLU99_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17813.1"
                     /translation="GDGGSTVIYPENAPAEVVTTIVTTITTFIDDDFENDLGWTVYNT
                     PPMTEGFWQRGVPGGFPDHSPAHDYDESGNCYVTDNRLNKDVDLGPTILTSPVFDLSA
                     MTDVYVRYARYIYCDDAGSGIPMDDDYLDVELSADGGNTWVLAEHVTGQTDWVYTQLR
                     VLDFVELTSQFQIRFSLADNPTNSLTEGGVDAVWLFDKACLEGPQYGLGDLNCDNAVN
                     VFDIDPFVLALTSGAGFEAYYAVYPDCDGMLADANGDGEVNVFDIDTFVQLLTGGSLR
                     "
BASE COUNT         1161 a         1998 c         2035 g         1235 t
ORIGIN      
        1 aacggcgcgg tgatgcccga ttcaacgctg gtcgtgccgg gcgccaccga cctgaccgta
       61 atcgcagtgg attcggccgg aaccggctac attgccgatc gcgacaccgc gaacgcggtg
      121 ttcatttatg acaacattgc gacgcgtaat ggaaccatcg cgcccgaccg cacgctgcag
      181 ggcgcaaaca cactattgca gcggccgatc agcgtcttta tcagcgagtg acgagacgcc
      241 gctgcggcag ttgatgctgg gcgcggtggg ccgcggcttc gtggttctct ttcagccgtc
      301 cgccggtcgc gccccactaa ggcgccgtca tccgactatc tgcactatga cctcgcggcg
      361 gcgcgggcgc gtgtcgaagt ccagcagcac gatttgctgc caggtgccga gcggcatgcg
      421 cccgtcgatg actggcagag cgagtgacgg cccgagcgcc gaagcgcgca catgcgcgtg
      481 gccgttgtcg tcgtgccagg tttcctcgtg cttgtagtag gcatcgcgcg gcgccacgcg
      541 ctcccagaac gccttcaggt cgtgcgtcag caggccgggt tcggcctctg tggtggtgat
      601 ggccgcggtt gagcccacta cgaaggccac caacaggccc tgctgcactg cggattcggc
      661 gacgatttcg gccacttggc tagtgatgtc aagcacttgc gagtcgcccg tggtggcgac
      721 tgtgaaccgg cccgaataag tcatgtcgac ctccgtcacg tggcgcgcgc aacagtcgct
      781 cgaacacccc cgctcgaagc ctgccgatag ccgccggtcc gccggcttct ctcggagcct
      841 actccaggta tcccaactcg gtcagccgct cttcgaggat gcgtcgatcg ctgtcgctgt
      901 aaacttcgac ggcctcttcc acagccttct tcagcggcgg ctcgcgttcg atgaccggat
      961 cggtggtaaa cgcctcgcga atgacgcggc cttccatgtc taccggcact cgcaagccca
     1021 atgccgccag catcgtcggc gcgatgtcga ttatgctcgc ttcggtgcgt tgtccgtgtc
     1081 gcacgttggc cccgcccagg gccaaaattc cttcgtcgcg gtgcgtgcct tccagccggg
     1141 ccgatttaca ccagcgcacc ggctggccgc cccgaatttt gcgaatcacc gccagaccgg
     1201 ggttcggcgc cagcagcaaa tcgggaaggt tcgggttctc gccccggtcg cagtgataca
     1261 gggtttccgt cttgtatacg tgcggaaaga tgcgcacctc gcgcccgttc cggtcgcgca
     1321 ccgacgcgct gcgcagccgc tcaatcagtt cgttgcgcag cggctcgtaa tcggccggct
     1381 cgacgatgcc gtgcggtccg cggcctttca ggttgatgta caagtagccg tagatgccgg
     1441 cgtgcatgac acacgcccgg gtgcgcgtcc agtcgaccgc cagctcgcgc tcgatgccgc
     1501 ggctgccctg ctcaaagcgg gtcgctttgc ccttggtaaa gcgatgccac cagtacgcgg
     1561 cgcgcgtgtt ggcctgctgc caggggctgc gcagggccag gtagccccag cgcttcaaca
     1621 gcaggttcgg ctgggccttg ccgtccaggc tgccatgccc gtggtcggac atgatcagca
     1681 ccgtcgcgtt gttccgctcg gcatactcga acatgcggcc gagcacgtcg tccaggcgcg
     1741 tgaaaacgcg ggcggccagc tctgcctcgc gcgggaagtt gccggcggtt tgcgggtcga
     1801 ggtacttcca agccttatgc tggagattgt cgaccagctt catgaccgtc atcagcacgt
     1861 cccagccgta cttgtcgccg caataggtcg tgaggcgata gccctgttcg aagctgttgg
     1921 cgatgtactc caggttggtc tcgagcacgt cctgcccgcc gagggcgcgg cgctgccagt
     1981 tggtgcggta gttgtagttc gggatttgct ggaaaatctc ctgcttcagt tcgcgcggat
     2041 aggtgaattc ggcgtcgatg ctgggcgtct cgaagccgct gatcataaag ccgttgatgg
     2101 gcttgggcgg gaacgtcatc ggcacattga tgctgccgac ccgcaaaccc ttttcgctca
     2161 gcagctccca aatcgtcttt tcctcgatct ggtaggtgct gttgaacgtc aactggtgct
     2221 ggaagacgtc gtacttctcg aaatcgagga tgccgtgctg cccgggccct ttgccggtca
     2281 tgaacgtcgt ccaggccgcc ggcgttatcg gcggttgcgt cgagtgcagc acgccggccg
     2341 tgccctgctc gatgaaacgc ttgaggttcg gcatgcggcc ggcgttcatc agcggatcga
     2401 gcacgtaaaa cgtcgcgccg tccaggccga tgatcaacag ccggtcggtc agtggtttca
     2461 ggggccgggg cgcccgcgac tgcgccgccg ggccgggtac atccgcaacg gaaacttctg
     2521 gctgcatcct ctcggctcct cgccggccat gccggacatg aagacccgtg ttcatgcttt
     2581 aacgaaaaaa ccgcgccttt cgggctaccg cgacggatac tcgtcgccgc ggaccggctg
     2641 ctcagcatac cctgttttgg ccgccgaatc atcctgctga acgaaaaacc ggtccgtggc
     2701 tttgggggca ttcgacagaa cgcgtcgaag ctggaaaaat gcgggcatgt ccgaaatgcc
     2761 tcgatggctg gccgagactc ccgtggattt tgccgcgatc gatagcgcgc cgcgcgacgc
     2821 gctgcgggcg cggctggaca tcgctgggga gcagaccgcc atcctcgtcc tgccgccgct
     2881 gacgcgcgcc gcaggcgctg aatacgccgt ttgggcggcg ttgttgcttg agaaagtgcg
     2941 gcccgatgta cgcctggtcg ttcccggccc ggctgaggag aatcggcgca ttcgctacct
     3001 ggtgcgctca tgccggcacc aatacatgat gcgtctggcg gaggagttat cgctggcgga
     3061 actggtcgcc gcggcggaca tcgcgacgtt cctcccgatc gccgccgttc ccctgaccgg
     3121 cctggcttgg gccatggcgg cgggccggac catcgtcgcc agcgaggtgc cgccgatggc
     3181 cgcgcttctg gaagccggcc ggacggcatg gctgtgccgg ccgcgcgatc cgcaggacgg
     3241 cgcccgatac atgctcaagg ctttggatga accggttgtt tcagcacgcc tggccgcggc
     3301 ggcgcgtgaa aaagcgctcg cgttgttctc ggcgccgcgg atggtcaagc ctacgaactg
     3361 taatggcgtt tgagctcgga ggcgtaatgt cgcccgaggt cggcggaatg ttggtcgagc
     3421 cagtcgctca gacgcgtttt gccggtgggg ggctggcctg atacgagcag gttcgccatc
     3481 agcccgtgca gctcgtcgcg cgtgagcatg acgtcgccga gcgccagccc actcatttct
     3541 gccgccagca acgcgatgct cggcggagca tgcgttatgt gggcccgggc cccaaccttg
     3601 cccgcgatca gacgcaccaa ttcctcgaac gtgaagattt ccggaccgac tgcatcaacc
     3661 gtgacgtccg cggtcccctg agcggcgctg acggcgattt cagccacgtc ctcgacgcac
     3721 acgggctgga tgcggtactt gccgtcaccg ggaatggcga acagcggaac atgccgcagc
     3781 agccaggcga tgttattaat caggatgtct tcgttgccaa agattacggt ggggcgcacg
     3841 atggccggcg gaacgcccaa gccggccagg aattcctcga gcagcccctt gccgcggaag
     3901 tacggcaacg gcgactggga tgaggggtta gtgatactga tgtggacgat gcgttgaacg
     3961 ccggcctcct tcgcgctcgt tagcaggatg cggctgttct caaccgcttg ctcgaatgtc
     4021 aggggaccgc gcgggaagcg aatccaatag gtgttgtata gcgtgtgtgc gcccgccatg
     4081 ctggccgcca gcttctcggg gtgcgaaaag tcgagcggcg cgatcgtcac ctggtcgccg
     4141 aacgggttct cgggtggcgg atgattggtc agggtaagca gacgccgccc ctgcgcgagc
     4201 aggcgccgcg cgatgtactt gccggtgtag ccgaacgcac cggtgacgac gtcgaaatcg
     4261 gattgcattg cgcgcctctc gtttcagttc gttcgggcgc gttcacgcgg cccaagcgga
     4321 cgcgcggcgg gcttatgcta gcctggcgca ttctatccgg tcaaatcgga tcaacgtggc
     4381 ctagtctgtg tgtgcccgtg acaaagtgat aaagtgataa ggtgataggg cgataaaggt
     4441 cgatctgcgg aagcgctgct gccggttgtg aagcgcttgc ttcgcgacgg agtgattcgc
     4501 tcagcctgta aaaaagggac accagccgcc cgtagactgc gccattgcgc caggacgggt
     4561 tgtttattcg aatacggtgc ccaccggcgc ggctaacata caggtactca tactctactc
     4621 cgtcgcacac accgtggtaa ggtatgggtg caagatcgtc aaccgcattg cgagccagga
     4681 attcgcgaaa atcacgccat tcttcatcag tcaggagacg cgaatgaggt tcagactcca
     4741 cactgcccaa gaacagccgc aattcggcct ggtcgcccgc gtggccccta cgtgcagcgt
     4801 gaccacgacc agcatcccca cgaataggcg cgatacaaac tgggaagtgg agtcacacat
     4861 ggcgactgtc ctccaaaggt agcgactgcg tcaaacaggc gcgatgagcc cattgtaacc
     4921 gcgccgcact tcaagtacgc aataggcgac taccatgata aggtggcgcc gggcgctcat
     4981 tacaaacagg agagacacat ggtgcggaac ctctggcagg atttcaagga tttcgcgttc
     5041 aagggcaaca tgctggactt ggccgtggcg gttgtgattg ggaccgcttt cggggccgtc
     5101 gtcaactcgc tggtcacaaa catcatcatg cctgccatca gctacgttca gcccagcggc
     5161 gagggatacc gggcctggca atggggccgc atcgagattg gcgcgttcct cggccagttg
     5221 gtcacattcc tgataatcgc tgcgacggtt ttcctgctaa tcgtcaagat tgtgggcacg
     5281 ttgatcaagc gcgtcagcac gccgcccgcg tcgcccgaac cggtgaccaa ggaatgcccc
     5341 ttctgcttga tgactattcc gagcaaagcc attagatgcg ggcactgtac cgctgacctc
     5401 agcccggcgg ccggcgccaa gtcagcgccc ggccttatgc ccggataacg cgtaattcgt
     5461 gtccatgggc agcggcgtca ggcatgcgag cagagcgggc ggtcgcgcct atcgcagata
     5521 aagagcgacc ggcgccaggc ttgcctgacg ccggtcgcaa tacgtagacc aaatttcagt
     5581 ggcgtgccgc gctattggtt aacgcaggct tccaccagtc aatagctgca caaacgtgtc
     5641 gatatcgaac acattgacct cgccgtcgcc gttggcgtcg gccagcatgc catcacaatc
     5701 gggatagaca gcgtagtacg cttcgaatcc cgcgccgctc gtcagagcca acacgaacgg
     5761 gtcaatgtca aacacattca ccgcgttgtc gcagttcagg tcgccaaggc catactgcgg
     5821 accttccagg caggccttgt cgaacagcca gacggcatca acgccgccct ccgtcagcga
     5881 gttggtgggg ttatccgcca gggagaagcg gatctggaat tgcgacgtca gctccacgaa
     5941 gtcgagcacg cggagttggg tatagaccca gtcggtctgc ccggttacgt gctccgccag
     6001 cacccacgta tttccgccgt ccgcggaaag ctcgacgtcg aggtagtcgt catccatcgg
     6061 aataccgctg cccgcgtcgt cgcaatagat gtagcgagcg taacgcacgt agacatccgt
     6121 catggcggac aaatcaaaca ccggcgaagt aaggatcgtc gggcccaagt cgacatcctt
     6181 gttgagacgg ttgtcagtaa cgtagcagtt gccggattcg tcgtaatcgt gcgccgggga
     6241 atgatccgga aaaccaccgg gcacgccgcg ctgccagaag ccctcggtca tgggcggggt
     6301 gttgtacaca gtccagccca agtcgttttc gaaatcatcg tcaatgaagg tggtaatggt
     6361 ggtcacgatc gtcgtcacca cctcagccgg cgcgttctcc gggtagatga ccgtcgagcc
     6421 gccgtcgcc
//
LOCUS       DAOSYS010000205         6406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10268_length_6406_cov_5.395686, whole genome shotgun sequence.
ACCESSION   DAOSYS010000205 DAOSYS010000000
VERSION     DAOSYS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10268_length_6406_cov_5.395686"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1706
                     /locus_tag="PLU99_11990"
     CDS             153..1706
                     /locus_tag="PLU99_11990"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOO17814.1"
                     /translation="MTLDLTMGIFIWLLGLCVGSFLNVVIYRWPRDLSIRRPSRSFCP
                     HCGAAIAWYDNIPVLSWLMLAARCRHCRAPISVQYPLVEAATGLTFVLVYYLLFIAGT
                     RLPPADHLQQPADWPLLAAWLVLAAALIACAAMDLAWYMVDIRITYVAMVAGLVLYAI
                     WRGGQFFEPIAPTASGAAALAALIVGVVTAWLANRRTPGIDLSTAEQPQDSEQTPPAA
                     RRAELTGGRLATVLMVVLAIWAFSEIALHRHAPGLMVIQADGKIAWGAPGEQIGGLAP
                     QLLAPAALVTLFAVLVLAGGQARHIDTEVEEALEHERSQARAQVLRELAWLIPALAAA
                     LGTFAILVWFPRVGQAWHQAAEWSVGPGIQLLAGLTYSAFGLMVGAAAGWLLRIVFTL
                     IFGREALGSGDIYILAAAGAAGGWDIALLGLLLAVGIALAAYAISLLLKRTLTIPFGP
                     WLALGFVAALWQNQRAALYAQQYYEAIRYAWTHQASVCWLGAGLMLIGSAAAIAAARL
                     VRRALEPRA"
     gene            complement(1776..3371)
                     /locus_tag="PLU99_11995"
     CDS             complement(1776..3371)
                     /locus_tag="PLU99_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO17815.1"
                     /translation="MRLPRISLANKYRILFGLAVLLIIGAALAVPWYWMELLVLKQPF
                     HEAQRIADDYYQLVLPQPGAATRASGPHSHEGLLPEKQDAHVRFVRLPLAQNDPELIA
                     AQVEMPLVATALKAFTRNPNQTSVYETVDEPDGRRLYYAHAVRATKRCMSCHDEAHNV
                     VFQENQLAGLITVDLPADLGSEGVFWNRVVLVVAGGLAGILAVLVFYVITQRFILSPI
                     HELRQVTLRVTEGDLTVRSNVQSGDEFEQLSANLNTMLERLRDSQEELQQANRLLDEK
                     LGQMAETNVALYEANRVKSEFLANVSHELRTPLTTIIGFAELLREGPHVEEGSRAARY
                     AENILISGRILLEIINDLLDLAKIEAGKMEVRVEPVNLAELCESMQILMKPLAEKKSL
                     QFDLLVDNDLPTLQSDQSKLRQILYNLVSNAIKFTPPGGQMRLAARRLDDEHVRLEVS
                     DTGPGIAPEHRATIFEKFRQIDQSPTREHHGTGLGLAIARELAHLLGGEIEVESELGH
                     GATFTVTLATAAPIAGTRPAILA"
     gene            3684..5837
                     /locus_tag="PLU99_12000"
     CDS             3684..5837
                     /locus_tag="PLU99_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOO17816.1"
                     /translation="MRKGAFMVGLLVAGLTADVFAQAKTSLVDRPSINPPNAFHIGNR
                     EPLWPVPLVKLPIGAIEPRGWLRTQLQLEADGFSGRLTEISEFLKKENNAWLSPSGEG
                     EHGWEEVPYWLRGFGDLGYVLRDERIITETRLWLEAVLAGQREDGYFGPRANLTRLDG
                     KPDLWPNMIMLNALQSYYEYSGDERVLKFMTNYFRWQLAVPEEDFLVTSWQHQRAADN
                     LDSVYWLYNRTGDAWLLDLAEKIHRRTANWTEAVANWHGVNIAQAFRGPAVYFQQSCD
                     LQQVLAACERYGEVMLTYGQVPGGGFAADENCRPGFSGPQQGTETCTWAELMRSFESM
                     LKITADPLWAERCEEIAFNSLPASMTADLKGLHYLTAPNLAACDSKDHAPGFENGGDM
                     LSFDPHKYRCCQHNVGQAWPYFAEHLWLAACNNGLAAVMYAPCVVKAKVGAGDGAEVT
                     ITEETRYPFDDSVTMVFSTAQAARFPLFLRIPSWCEEAEIEVNGVSEWRGAIRRPGAY
                     VCVERVWSDGDRVELRLPMKTRIRRWPANGDSVSIERGPLTYALKIGAKQVRSGGTDA
                     WPAYELLPTTPWNYGLVLDKQEPAASFELLTKDWDGIQQPFDGDTAPLELRARAARIP
                     GWQLDEHGLVGKLSPSPVKSDQPVETVTLIPMGCARLRIAAFPTIGTDPDAREWGDAP
                     AASGATPPAVTPTASAPNAAPAATNAAPAATDPAR"
     gene            complement(5937..>6406)
                     /locus_tag="PLU99_12005"
     CDS             complement(5937..>6406)
                     /locus_tag="PLU99_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOO17817.1"
                     /translation="LAGRAVGGFYTGEIRIAGQRQGFEVSTFSGRVAVLAHVETHSPH
                     RVGRYGVDVAAFERTVLPELERRADVLLIDEIGKMECFSARFVQAVRELLNGNVPLVA
                     TVALSGTGFIAEVKRRPDVRLWQVTTANRDELPKRLAEQVFAALARGEERPVA"
BASE COUNT         1103 a         2115 c         2021 g         1167 t
ORIGIN      
        1 ctggaagaaa tgcgccaagt acttgcacag cggcggcctt tctacgaggc tttggcgcaa
       61 caaatggtcg acaccgacgg ccgcacgcca caggacgtgg ccgctgagat attggcccgc
      121 ctgggtgcct ggccggccgt gacggaaaaa tgatgacgct cgacctgacg atgggcattt
      181 ttatctggct gctcgggctg tgcgtgggca gcttcctcaa cgtcgtgatc tatcgctggc
      241 cgcgcgacct gtcgatccgc cgacccagtc gctcgttctg cccgcactgc ggcgcggcca
      301 tcgcctggta cgacaacatt ccggtcttga gctggcttat gctggccgcc cgttgccgtc
      361 attgccgcgc accgatctcc gtgcagtacc cgctggttga agccgctacc gggctcactt
      421 tcgtgctcgt gtactacctg ctcttcatcg ccggcacacg cctgccaccc gctgaccacc
      481 tgcaacagcc ggccgattgg ccgctgttgg ccgcatggct ggtgctggcg gccgcgctca
      541 tcgcctgcgc cgccatggac ctggcgtggt acatggtgga tatccggatt acgtatgtgg
      601 ccatggtggc cggcttggtg ctgtatgcca tctggcgggg cggtcagttc tttgagccca
      661 tcgctccgac ggcctccggc gccgccgctt tagccgcgct gatcgtcggc gtcgtcaccg
      721 cctggctggc gaaccgccgc acgccgggca tcgatctttc cacggcggaa caaccgcagg
      781 acagtgagca gacgccgccg gccgcgcgcc gcgcggaatt gaccggcggg cgactggcga
      841 ccgttctcat ggtcgtgctt gcgatctggg ccttctcgga gatcgctctg catcggcacg
      901 caccggggct aatggtcata caggctgacg gcaaaatcgc ctggggcgcc ccgggcgaac
      961 agattggcgg cctagccccg caactactcg cacccgccgc gctcgttacg ctctttgccg
     1021 tgcttgttct ggcaggcggg caagcgcggc acatcgacac cgaggtagaa gaggcgctcg
     1081 aacacgagcg atctcaagca cgtgcccaag tgctgcgcga actggcctgg ctgattccgg
     1141 ccttggccgc cgcgctcggt acgttcgcta tcctcgtatg gtttccacgc gtcgggcagg
     1201 cctggcacca ggcggccgaa tggtccgtcg gtcccggcat tcaactgctg gccggcctga
     1261 cctactccgc gttcggcctc atggtcggcg ctgccgccgg ctggctcctg cgaatcgtct
     1321 tcaccctgat ttttggccgc gaagcgctcg gctccggaga catctacatc ctggccgcgg
     1381 ccggcgcggc gggcggctgg gacattgccc tgctcggatt actgctggcg gtgggcatcg
     1441 ccctggccgc ctacgccatc agcctgctgc tcaagcggac gctgacgatc cccttcggtc
     1501 cctggctggc gctggggttc gtggccgcgc tgtggcagaa ccagcgtgcc gcgttgtacg
     1561 cccagcaata ctacgaggcc atccgctatg cgtggaccca ccaggcgagc gtctgctggc
     1621 taggggccgg gctgatgttg atcggctcgg ccgccgcgat cgctgcggcc cgcctggtcc
     1681 ggcgcgccct cgaaccccgc gcttgatgtt caattagacg agaaaaattg gaagactcgt
     1741 cgtaaatgcc ctgtttcacc tcgccggcgg acccctcagg cgagtatggc ggggcgggtg
     1801 cctgcgatgg gcgctgccgt tgcgagagtt acggtgaaag tcgcgccgtg gcctaattcg
     1861 ctttccacct cgatctcgcc gcccagcagg tgggccagct cgcgggcgat tgccaggccg
     1921 agcccggttc cgtggtgctc gcgcgtcggc gattggtcga tctgccggaa cttctcgaag
     1981 atcgtggcgc ggtgttccgg ggcgatgccg gggcccgtgt cgctaacctc cagacgcacg
     2041 tgctcgtcgt ccaagcgccg cgccgccagg cgcatctggc cgccgggcgg agtgaacttg
     2101 atggcgttcg agacgaggtt gtacagaatc tgccgcagct tgctctgatc gctctgcagc
     2161 gtcggcagat cgttatcaac cagcaagtca aattgcaggc tcttcttttc ggccaggggc
     2221 ttcatcagaa tctgcatgct ctcgcacagc tcggccagat ttaccggctc gacgcgcacc
     2281 tccatcttgc cggcttcgat cttggccagg tcgagcaggt cgttgatgat ctccagcaga
     2341 atgcggccgg agatcaggat gttctcagcg tagcgggcgg cgcgcgagcc ctcctccacg
     2401 tgcggccctt cgcgcaggag ttcggcaaag ccgatgatcg tggtcagcgg cgtacgcagc
     2461 tcgtgcgaga cattggcgag gaactcgctc ttgacgcgat tggcttcgta cagcgcgacg
     2521 ttggtttccg ccatctgccc cagcttctcg tccagcagcc ggttggcctg ctgaagctct
     2581 tcctgtgagt cgcgcaagcg ttcaagcatg gtgttaaggt tggcggaaag ctgctcgaac
     2641 tcatcgccgc tctgcacgtt cgagcgcacc gtcagatcgc cctcagtcac gcggagcgtc
     2701 acctgccgca gctcgtgaat cggcgacaaa atgaaccgct gcgtaatcac atagaaaacc
     2761 agcacggcca aaatgccggc cagcccgccc gccaccacca gcaccacgcg attccagaac
     2821 acgccctccg accccagatc cgccggcaaa tccacagtga tcaaccccgc aagctggttt
     2881 tcctgaaaca cgacgttgtg cgcctcatcg tggcagctca tgcagcgctt cgtggcccgc
     2941 accgcgtggg cgtagtacag ccgccggccg tccggctcgt cgaccgtctc atagaccgac
     3001 gtctggttcg ggttgcgcgt gaacgccttc aacgccgtgg ccaccagcgg catttctacc
     3061 tgggccgcga tgagctccgg atcgttctgc gccagcggca gccgcacgaa gcgcacgtgc
     3121 gcatcctgct tttccggcag caaaccctca tggctgtgcg gcccggacgc acgcgtggca
     3181 gcgcccggct gcggcagaac caactggtag taatcgtccg cgatgcgctg ggcttcgtgg
     3241 aacggctgct tgagcaccag cagttccatc cagtaccagg gcacggccag ggccgcaccg
     3301 ataatcagca gcacggccag gccgaagagg atgcggtact tgttcgccag cgatattcgc
     3361 ggaagccgca tgcgtgtgcc ggtcctggtg agaacaggac gtcggccggt cgcccgcgat
     3421 caggccaggg gccgacgcta cgtgtctccg gtggcgatag ttagcacgta cgcagcggca
     3481 ctgcaactgg gggacgctga atcggcggcg ccgggtatcg gacgaaagac aagggggcat
     3541 gcccgcgctt acagcttggg catggcaccc gcggcaccca gcgccgtttg cgcgcgcggc
     3601 gatgctatgg cctcaaactt gccgcggggc gtcatttcgt tatcgttttc gaagattacc
     3661 cgagccgctt tcaggagcag ttcatgcgca agggagcgtt catggttggt ctgttggtcg
     3721 ccggtctgac ggccgacgtt ttcgcgcagg caaaaacgag tctcgtcgat cggccgtcaa
     3781 tcaatccgcc caacgcgttt cacatcggaa atcgcgagcc gctctggccc gttccgctgg
     3841 tgaagttgcc gatcggggcg atcgagccgc gcggctggct gcgcacccaa ttgcagctcg
     3901 aagcggacgg cttctccggg cgcctcacgg agatcagcga gttccttaag aaggagaaca
     3961 acgcctggct gagccccagc ggcgaggggg agcacggttg ggaggaagtc ccctattggc
     4021 tgcggggctt cggcgatttg ggctatgtgc tgcgtgacga acgtatcatc accgaaacgc
     4081 gcctctggct ggaagccgtg ctggccggcc agcgtgaaga cggctacttc ggaccgcgcg
     4141 ccaacctgac gcgcctcgac ggcaagcccg acctgtggcc gaacatgatc atgctcaacg
     4201 cgctccagtc gtattacgag tacagcggcg acgagcgcgt cctaaagttc atgaccaact
     4261 acttccgctg gcaactggcc gtgccggaag aagacttcct ggtcacctca tggcagcacc
     4321 agcgcgctgc cgacaacctc gacagcgtat attggctcta caaccgcacc ggggatgcct
     4381 ggttgctgga cctggccgag aaaattcatc gccgcaccgc gaattggacg gaggcagtgg
     4441 cgaactggca cggcgtgaac atcgcccagg ctttccgcgg tcccgccgtt tactttcagc
     4501 agtcctgcga cctacagcag gtcctggccg cttgcgagcg ctatggcgaa gtgatgctga
     4561 catacggcca ggttcccggc ggcggctttg cggccgacga gaactgccgc cccggcttca
     4621 gcggcccgca gcaggggacg gagacctgca cctgggccga actcatgcgg tccttcgaaa
     4681 gcatgctcaa aatcacggcc gatccgctct gggccgaacg ttgcgaggag atcgcgttca
     4741 actcgctgcc ggcttccatg acggccgacc tcaaaggatt gcactatctg acggcgccga
     4801 atctggctgc ctgtgattcg aaagaccacg ccccgggctt cgaaaacggt ggcgacatgt
     4861 tgtccttcga tccgcacaag tatcgctgtt gccagcacaa cgtcggccag gcctggccgt
     4921 actttgccga gcatctttgg ctggcggcct gcaataacgg tttggcggcg gtcatgtacg
     4981 cgccgtgcgt ggtcaaagcc aaggtgggcg cgggcgacgg agccgaggtg actatcaccg
     5041 aggagacgcg ttatccgttt gatgattcgg tcaccatggt gttctcgaca gcgcaggctg
     5101 ccaggttccc gctgttcctg cgaatcccca gttggtgcga ggaggctgag atagaggtca
     5161 acggggttag cgagtggcgg ggggccatcc gccgcccggg cgcttacgtc tgtgtcgaac
     5221 gcgtctggtc agatggtgat cgcgtcgagt tgcggctgcc gatgaagacc agaattcgca
     5281 ggtggccggc aaacggcgat tccgtctcga tcgaacgcgg tccgttgact tatgcgctga
     5341 agatcggcgc caagcaggtc cgctcgggcg gcacggatgc ctggccggct tacgaactgc
     5401 tgccaaccac gccttggaat tacgggttgg ttctcgacaa gcaagagccg gccgcatcat
     5461 tcgagctgct tactaaagac tgggacggga tacagcagcc gtttgatgga gataccgccc
     5521 cgctcgagct gcgggcccgc gccgcccgga tacccggctg gcaactggac gagcacggcc
     5581 tggtcgggaa actgagcccc agcccggtga aatcggacca gccggtcgag acggtaaccc
     5641 tgatcccgat gggctgcgcg cggctgcgca ttgcggcgtt cccaactatt ggaacagacc
     5701 cggacgcacg tgaatggggc gatgccccgg ccgcatccgg agccactcct ccggccgtaa
     5761 ctcctactgc atccgcgcct aatgccgcac cggccgcaac taatgccgca ccggcggcaa
     5821 ctgatcccgc gcggtaggaa actccccggg tgccactgct tgaagccgca gcttcaagca
     5881 atgagattcc aagccgcggc gcccgcaagc accaacgctc gcaggcagaa gcgcccttat
     5941 gcaaccgggc gctcttcacc gcgggcaagc gccgcaaata cctgttccgc aagcctcttc
     6001 ggcagctcgt cgcggttcgc ggtagtcacc tgccacaacc ggacatccgg ccggcgctta
     6061 acctcagcta tgaagcccgt gcccgaaagc gccacggtag ccacaagcgg cacgttgccg
     6121 ttcaacaact cgcgaaccgc ctgcacgaac ctggccgaga agcactccat cttgccgatt
     6181 tcgtcgatca gcaacacgtc cgcccggcgc tccaactccg gcaatacggt ccgctcgaac
     6241 gccgcaacgt ccaccccata gcgcccgacg cggtgcgggc tgtgcgtttc cacgtgagcg
     6301 agtaccgcaa cccggccgga gaacgtgctg acttcaaagc cctgccgctg gccggcgatg
     6361 cgaatttcac cggtgtagaa accgcccacg gcccggcccg ccagaa
//
LOCUS       DAOSYS010000206         6395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10282_length_6395_cov_4.680757, whole genome shotgun sequence.
ACCESSION   DAOSYS010000206 DAOSYS010000000
VERSION     DAOSYS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10282_length_6395_cov_4.680757"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..1572
                     /locus_tag="PLU99_12010"
     CDS             334..1572
                     /locus_tag="PLU99_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17818.1"
                     /translation="MLRLAWANLMHRKLRTTLSVLAVGIGIALLLVSKGLANGSIAEV
                     NQRMQSVDAQLVVLPAQENILFTNGAPFPGVFERLLARQADEHGPLATAVIPVFFAQV
                     RMGGQQQRVFGVDPRQMSLFLGSRRVLEGRAFDQAFSMAEKTAELRRPTTQIVEAESL
                     PPEILEAGLELIIDDRLRRVGEFDAASGTNRPYRIGDEIRIMSRGFRIVGVVETGVAG
                     RVFAPIQTLREILNGGEPWSTMFFVKLRPDLDPVAAADRFQSVLGQQARVELKSEYGK
                     LLHESFAQVNMYMTASSGLALVVCFLFILLTMYTMVLERTREIGILKALGMTRLSLLS
                     LSISEALVVSSSGALIGIGLAYGARYVLNVLRPLLTVELPLSMLAVALGLAVIGGGLS
                     ALYPGYRAARLEPAVALTCE"
     gene            1614..2294
                     /locus_tag="PLU99_12015"
     CDS             1614..2294
                     /locus_tag="PLU99_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17819.1"
                     /translation="MTPELMIDACNLHHAYSHGAMVEPVLHGINLRIRRGEFVVIMGP
                     SGCGKTTLLNLLGLMMLPTQADRLEIAGVDTRHLSDAARTRLRREAIGFVFQRFNLLP
                     TISAYRNVVLPLHLRGIQPDGQAAELIRRTGLQELVHKKPAQLSMGQQQRFAIARALV
                     GRPALLLADEPTGNLDSANAEAVLSLLSEFHRDWGQTIVMITHNEHLTVAADRVLRMC
                     DGRIIDGA"
     gene            2284..3210
                     /locus_tag="PLU99_12020"
     CDS             2284..3210
                     /locus_tag="PLU99_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17820.1"
                     /translation="MAPDPLAAEPATGRQAQASSAGSQAGRCPAQGGCHAQAVSAGMP
                     DVNGVKVAPRDGAEACERAERPSRAEPSVQISDSLSPAREQELPGLRLWFLIYLAWLA
                     GLAALAHVGSVQLELGSPGAMGLWTLSLMAFYLSLCNLFLPLPTAWIILLAASPDGGA
                     FGSAWQRVAAVTVVGAAATAIANVNEYHVLSYVLGHGLGGKLRRTRVYEWAVRWFDAA
                     PFQVLALIGFVPVPIDAVRWLAILRRYSRWRFGLAYFVGRGLRYLLFAGFAVLASLSG
                     KEILLIQAGIVVAALLARFAWPRLRGGRSAIA"
     gene            complement(3437..3655)
                     /locus_tag="PLU99_12025"
     CDS             complement(3437..3655)
                     /locus_tag="PLU99_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17821.1"
                     /translation="MDLGEELSALFGGHYVDIVNPRYLDQRIRESVLRSALLQYEAAH
                     DDDDTRLVWPLQTAASPPRPVPTRRRSS"
     gene            complement(3694..3840)
                     /locus_tag="PLU99_12030"
     CDS             complement(3694..3840)
                     /locus_tag="PLU99_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO17822.1"
                     /translation="MFKDRPQPRIPIDGAKLAALCQRWHIARLSLFGSVLRDDFRPDS
                     DVDV"
     gene            complement(3965..>5787)
                     /gene="dnaK"
                     /locus_tag="PLU99_12035"
     CDS             complement(3965..>5787)
                     /gene="dnaK"
                     /locus_tag="PLU99_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOO17823.1"
                     /translation="QQDGATHVVMEVLVGQRARNQQVTNPHNTVFSIKRFMGRRHNEV
                     STEEKMVPFKIVGGSDELVRVHIDATNKDYTPPEISAFILRDLKKTAERYLGEKVERA
                     VITVPAYFNDSQRQATKEAGQIAGLQVERILNEPTAAALAYGLERKVNEKIAVFDLGG
                     GTFDISILDVGDNVFEVLSTNGDTHLGGDDYDEVLINYVAEEFRKKEGIDLRKDPMAL
                     QRLKEACERAKCELSGSMETSINLPYITADASGPKHLQMNITRAKFEQLSDQLTERCR
                     GPVMRALQDAKLSPKDIDEVVLVGGSTRTPRVQQLVKEIFGKEPNRSLNPDEVVAMGA
                     AVQAAVLSGEKSDIVLLDVTPLSLGVETLGGVMTVLIPRNTTIPTSKKEIFSTAADNQ
                     PAVDIHVLQGERKMAGDNRTLGRFQLTGIPPAPRGLPQIEVTFDIDANGILNVSAKDL
                     GTGKEQSIEIKASSGLSEQEVQRMVKDAEAHAAEDERKKKLIDVRNQADQVVYSTEKT
                     LKEHGDKVDAATRGEIEQAINRLKDAQKTDDAGTIEKAIENLTQVSFKLGEAVYKATG
                     KGPEAGGPPPGAGAAAGDAGRPGGKEEDVIDAEYEVKEGK"
     assembly_gap    5788..5797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5798..5940)
                     /locus_tag="PLU99_12040"
     CDS             complement(<5798..5940)
                     /locus_tag="PLU99_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOO17824.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGQPKVLINESGSRLTPSVVAFTE
                     KGEV"
     gene            complement(6240..6374)
                     /locus_tag="PLU99_12045"
     CDS             complement(6240..6374)
                     /locus_tag="PLU99_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17825.1"
                     /translation="MVPVSDENGACWAVLDLDSHQVGAFSEADVSGLLSVLRAAALTT
                     "
BASE COUNT          963 a         2069 c         2099 g         1254 t
ORIGIN      
        1 acagaaaaag cgggccaggg gctcgggtgc ccatgtagag gtacgagggc ggcggtctcg
       61 ggtgccatgc ccaagctgta agcgcgggca tgcctatgtt caggaactca cgaggcgcgg
      121 cacggttttg tgagcacgcc aatcacggcc cttggcagaa cgctcggggg gcctttggtc
      181 agggcatgcc cgcgccgcca cgcggcttgg gcatggcacc cgcccggacc ttgccgctcg
      241 cgattggcgt ttcgcaggcc gcccgcgccc gtttgacgca cgcccccgca gtccgtaccg
      301 ttttcagggc agcggaaggg agaaagtttt tcgatgctga gacttgcttg ggccaatctg
      361 atgcaccgca agctgcgcac cacgctcagc gtcctcgccg tgggcatcgg catcgccctg
      421 ttactggtct ccaagggcct ggccaacggc tccatcgccg aggtcaacca gcgcatgcag
      481 agcgtcgacg cccagctcgt cgtcctgccg gcccaggaga acatcctctt caccaacggg
      541 gccccgttcc ccggcgtctt cgaacgcctg ctcgcacgcc aagccgacga gcatgggccg
      601 ctcgccaccg ccgtcattcc ggtcttcttc gcacaggtgc gcatgggcgg ccagcagcaa
      661 cgcgtgttcg gcgtcgaccc gcgccaaatg agcctgttcc tcggctcacg ccgcgtgctc
      721 gaaggccgcg ccttcgacca ggccttctcc atggccgaga agacggccga gctgcgccgc
      781 ccgactacgc agatcgtcga ggccgagtca ctgccgcccg agatcctaga ggccgggctg
      841 gagttgatta tcgacgaccg gctgcgccgc gtgggcgagt ttgacgccgc gagcggcacg
      901 aatcgcccgt atcgcatcgg cgacgaaatt cgcatcatga gccgcggctt ccggatcgtc
      961 ggcgtggtcg agacgggcgt ggccggccgc gttttcgcgc ccatccagac cttgcgcgaa
     1021 attctcaatg gcggcgaacc ctggtcgacc atgttctttg tcaagttgcg tcccgatctt
     1081 gacccggtgg cggcggcgga ccgctttcag agcgtgctcg gccagcaggc gcgggtcgag
     1141 ctgaagagcg aatatggcaa gctgctgcac gagagcttcg cgcaggtgaa tatgtacatg
     1201 accgcctcca gcgggctggc cctggtcgtg tgtttcctgt tcatcctgct gacgatgtac
     1261 acgatggtac tggagcgcac gcgcgagata ggcatcctta aagcgctcgg catgactcgc
     1321 ctgagcctgc tgagtttgtc tatcagtgag gcgctcgtcg tttccagcag cggcgcgctg
     1381 atcggcatcg gcctggcata tggggcgcgc tacgtcctga acgtgctgcg cccgctgctc
     1441 accgttgagc tgccgctgag catgctggcc gtggcgctcg gcctggctgt aattggcggc
     1501 ggcctgagcg ctctgtaccc tgggtatcgc gccgcgcggc tggagccggc ggtcgccttg
     1561 acttgtgaat aacggcagag cttttgtgcg agcgagctag agctgacgtg caaatgacac
     1621 ccgagctgat gatcgacgcc tgcaacctgc accacgccta ttcgcacggg gctatggtcg
     1681 agccggtctt gcacggcatc aacctgcgca tccggcgcgg cgagttcgtg gtcattatgg
     1741 gcccgtccgg ctgcggcaaa actacgctgt tgaacctgct cggcttgatg atgctgccca
     1801 cgcaggccga ccgcctggag attgccggcg tcgatacgcg gcacctgtcc gacgcggccc
     1861 gaacgcgctt gcggcgcgag gcgatcggct ttgtgtttca gcggttcaac ctgctgccga
     1921 ccatcagcgc ctaccgcaac gtggttctgc cgttgcactt gcgcggcatc cagcccgacg
     1981 gccaggccgc cgagctgatc cgccgcaccg gcctgcagga attggttcac aaaaaacccg
     2041 cccaactctc gatggggcag cagcagcgct tcgccatcgc ccgcgccctg gtcggccgcc
     2101 cggcgctgct gctggcggac gagccgaccg gcaatctgga ttcggcgaat gccgaggccg
     2161 tgctgagttt gctcagcgag tttcaccgcg actggggcca gactatcgtc atgatcacgc
     2221 acaacgagca cctgacggtc gccgccgacc gcgtgctgcg catgtgcgac ggacggatca
     2281 tcgatggcgc ctgacccgct cgccgccgag ccggcgaccg gccgccaagc ccaagcctcc
     2341 agcgccggca gtcaggccgg ccggtgccct gcccaaggcg ggtgccatgc ccaagctgta
     2401 agcgcgggca tgcccgatgt gaacggcgtc aaagtggcgc cgcgcgacgg tgcagaagcc
     2461 tgcgagcgcg ccgagcgtcc ttcccgcgcc gaaccgtcag ttcaaatcag tgactctctc
     2521 agtcccgccc gtgagcagga actccccggc ctgcgcctgt ggttcttaat ttacctggct
     2581 tggctggccg gcctggccgc tttggcgcac gtgggttccg ttcagctcga actcggctca
     2641 ccaggcgcaa tgggactgtg gacactgtcc ctgatggcgt tttacctctc gctctgcaac
     2701 ctgttccttc cgctgccgac cgcctggatc attctcctgg ccgcctcgcc cgacggcggc
     2761 gcattcggct cagcctggca gcgcgttgcg gccgtaacgg tcgttggcgc cgcagcgacc
     2821 gctatagcga acgtcaacga gtaccacgtg ctctcatacg tgctcggcca tggtcttggc
     2881 ggcaaactgc gccgcacgcg cgtgtatgag tgggcggtgc gctggttcga cgccgctccg
     2941 tttcaggtgc tggcgttaat cggcttcgtg cccgtgccga ttgacgcggt tcgctggctg
     3001 gcaatcctgc gccgctactc gcggtggcgt ttcggcctgg cctatttcgt cgggcgcggc
     3061 ctgcgctacc ttttgttcgc gggtttcgcc gtgctggcca gcttgagcgg gaaagaaatc
     3121 ctgctgattc aggcgggcat cgtggtggcc gcgttgctgg cgcgtttcgc ctggccgcgg
     3181 ctacgggggg ggcgatcggc gatcgcctag tggaatcgcg ccgggcgacc ggttgcgctc
     3241 gcttgcgctt cgggctcgga ccgtgcgggc tcggatagag gggggcgcca agaagcgcag
     3301 cggattgcgc gggtggcgag gctgccggcg gcattccggg gcggcacgcc ccggctctgc
     3361 tccaacttgg ggcgcggcgt tttattccgc ttctgcatac acgcccggac tcaatcggcg
     3421 ggtgggattc gcaggtctac gacgagcggc gcctggtcgg aaccggccgg ggtggcgagg
     3481 cggcagtctg taatggccaa actagccgag tgtcgtcgtc atcatgggcc gcctcgtact
     3541 ggaggagcgc ggatcgcagt acggactcgc gaatgcgctg gtccaggtag cgcgggttga
     3601 cgatgtccac gtagtgtccc ccgaacagtg cgctcagttc ctcacccagg tccacgatct
     3661 cccagcccgg cgtgtgacca ggttcaaact ggatcagacg tcgacgtcgc tgtcgggacg
     3721 aaaatcgtcg cgcaagaccg agccgaagag cgacaggcgg gctatgtgcc accgctggca
     3781 cagcgccgcg agcttggcgc cgtcgattgg gattcgcggt tgtgggcgat ctttaaacat
     3841 cactgagagc gtacgccgag aggcggctaa aacacaaccc ggcccttgcg ggccgggtaa
     3901 tgatcggagg cacggcccac ttcgaacgtc ggccatggcc aacgtcggcc acggggggcc
     3961 ggtgctactt gccttccttt acctcgtact ccgcgtcgat gacgtcttct tcctttccac
     4021 cgggccggcc agcgtcgccc gctgcggcgc cggcgcctgg aggcgggccg cctgcttcgg
     4081 ggcctttgcc tgtggctttg tagaccgctt cgcccagctt gaacgaaacc tgcgtcaggt
     4141 tctcaatcgc cttctcgatc gtgccggcgt cgtcggtctt ctgggcgtcc ttcaggcggt
     4201 tgatggcctg ctcgatctca ccgcgcgtgg ccgcgtcgac cttgtcgccg tgctccttca
     4261 acgtcttctc ggtggagtag acaacctggt cggcctggtt gcgaacgtcg atcagcttct
     4321 tcttgcgctc gtcctccgcc gcgtgcgcct cggcgtcctt gaccatccgc tgcacttcct
     4381 gctcactcag gccgctggac gccttgatct cgatcgactg ctccttgccg gtgccgaggt
     4441 ccttggccga gacgttcaga atgccgttcg cgtcgatgtc gaaagtcacc tcgatctgcg
     4501 gaaggccgcg cggggccggc gggatgcccg taagctggaa ccgccccaag gtccggttgt
     4561 cgccggccat cttgcgctcg ccttgcagca cgtggatgtc caccgccggc tgattgtcgg
     4621 ccgcggtcga gaagatttcc ttcttgctgg tcgggatggt cgtgttgcgc gggatcagca
     4681 cggtcatcac gccacccaac gtctccacgc ccagcgacag cggcgtcacg tcgagcaaca
     4741 cgatgtccga cttctcgccg gacaagacgg ccgcctgaac ggccgcgccc atggccacca
     4801 cctcgtccgg attgaggctg cggttgggct ccttgccgaa gatttccttg acgagttgct
     4861 gaacgcgcgg cgtacgggtc gagccgccga ccagcaccac ctcgtcaatg tccttcgggc
     4921 tcagcttggc gtcctgcaac gcccgcatca ccggcccgcg gcagcgctcc gtcagttggt
     4981 cgctgagctg ctcgaacttg gcccgcgtga tgttcatctg caggtgcttg gggccgctcg
     5041 cgtcggccgt gatgtacggc aggttaatgc tcgtttccat gctgccggac agctcgcact
     5101 tggcccgctc gcaagcctcc ttgagacgct gcagagccat cgggtccttg cgaaggtcga
     5161 tgccctcctt cttgcggaat tcctcggcca cgtagttgat cagaacttcg tcgtaatcgt
     5221 cgccgcccag gtgcgtatcg ccgttcgtgc tgagcacctc gaaaacgttg tcgcccacgt
     5281 cgaggatgga gatatcgaac gtgccgccgc ccaggtcgaa gacggcgatc ttctcgttca
     5341 ccttgcgctc cagcccgtag gccagggcgg ccgcggtagg ttcgttcaga atgcgttcga
     5401 cctgcaagcc ggcgatctgg ccggcctcct tggtggcctg gcgctgcgag tcgttgaagt
     5461 aggccggtac ggtgatgacc gcccgttcga ctttttcgcc gaggtagcgt tcggcggtct
     5521 ttttcaggtc gcgcaggatg aaggcggaga tttcgggcgg ggtgtagtcc ttgttggtgg
     5581 cgtcgatatg gacgcgcacc aactcgtcgg agccgccgac gatcttgaag gggaccattt
     5641 tctcttccgt gctgacttca ttgtggcgcc ggcccatgaa gcgcttgatg gagaaaacgg
     5701 tgttgtgtgg gttcgtgacc tgctggttcc gggcacgctg gccgaccagt acttccatta
     5761 caacgtgggt cgcgccatcc tgctgggnnn nnnnnnnact tcgcccttct cggtaaacgc
     5821 caccaccgaa ggggtcaggc gcgaaccgga ttcgtttatc agcaccttcg gctggccgcc
     5881 ttccatcacg gcgaccaccg agtttgtcgt tccgaggtcg attccgatga ttttcgacat
     5941 tttttgctcc taatgaccgc gcagcccgcg caggggcagg cccgaaaacg actgcaaagg
     6001 cggttccaga ggtcgggctc ggggttattt cggtgtaagt tccgcaataa caagggtttg
     6061 cggcggcctg ggcgggggtg gggatgctgg gccagcgttg cttgctctgc caatctgaca
     6121 gggtaggtca tacacgtcag tgccacggcc aggatttcgg agccgggcgc agaagggggg
     6181 tgacgggttg gatacgcggg gggcgaccgt agcgcgccct gacatttccg gaagcgtttt
     6241 caggtggtca aggcggcggc gcggagcacc gataggaggc cgcttacgtc ggcctcggag
     6301 aacgctccga cctggtggct gtcgaggtcc aggaccgccc agcaggcgcc gttttcgtca
     6361 gagaccggca ccacgaccta tgattggtcg tgcgg
//
LOCUS       DAOSYS010000207         6353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10373_length_6353_cov_5.240394, whole genome shotgun sequence.
ACCESSION   DAOSYS010000207 DAOSYS010000000
VERSION     DAOSYS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10373_length_6353_cov_5.240394"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="PLU99_12050"
     CDS             <1..1365
                     /locus_tag="PLU99_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17826.1"
                     /translation="LQMGNAVSSWQLGAEGTEIDYGSAWDAAALSELRQDIQTFVAFP
                     QIVVPKSVFGPAVDQDAPGTGQRRRETAEVVSIYVPPHAPTRGLPVQLARHASAGAPW
                     IMLETDDALPVESRIVECARRLTLAKAAGAERVFLPAPLDRDPAMGRWCPNELYVPLR
                     TLCHFLGGKQATAVLPAANEDTLAILFTDGQEAGCVVAWSWCDRSGPPVAICGGEGAE
                     VWDLWGRRRPGGAGERLVVPLEAAPVIIAPVEAAAVAVQANLAIEPNLIDVDGSDARP
                     ILKLTNPFAEEMAGVMTVKVPPYWRAAESESAVQLAPGQSLEHALDLALPPREIAGEK
                     HIGVQLKLSLPRPMTLTVSIPVRLGLSGASFEAVALWRGPDLVVEQVVRNTGRTTLDA
                     VCSCQAAGRCRLEGALLGIAPEQTTTKVFEFLDARELAGSSLLVGLEKPNGRRLETVV
                     AVPP"
     gene            1634..2911
                     /gene="ricT"
                     /locus_tag="PLU99_12055"
     CDS             1634..2911
                     /gene="ricT"
                     /locus_tag="PLU99_12055"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOO17827.1"
                     /translation="MVAQQVPPNAAVARTDDERPATGNSSGRPSPPTALVRYGAMAWV
                     GEFTCRPGARTNCHMPVVVQTDRGIELGYPLLFCNSNCPLAIPKETIRNYIQNSGPEF
                     CRANAGRVLREATAQDVDEHLRLNAHPKEDLELCADLAANLGLDIKIVAAEHLLGGER
                     IVFYFRSEGRVDFRELVKELAHRFHTRIEMRQVGARDEARLLADWEVCGRECCCKSFL
                     KKLRPVNMKMAKLQKSTLDPSKVSGRCGRLRCCLRFEHEGYTELAAKLPRRGSRVRTR
                     MGTGTVIDHQVLTQLVLIRTDELNECAVPLEEILECDLPRPRAEAPAADERGKPASTE
                     APARSSGREADRAAQPRRAPGKPSGSHSPRTPTEPPSSGRAAHDSGDEPVEIETKDSD
                     ASSAQPRVVRRRRGGRRRNRRRPGADREPAPEN"
     gene            2950..3345
                     /locus_tag="PLU99_12060"
     CDS             2950..3345
                     /locus_tag="PLU99_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17828.1"
                     /translation="MKTEATQRLIETIRSDGRYPIEAYEFLHDGLEYTTRSLYGRVTP
                     NEPQHVTGQELCEGLRDLARERWGPLAGLVLRHWNIYRTRDFGEMVYFLIDLNLMGRQ
                     ESDRIEDFDDCFDLRTAFDSYRIPLDVYD"
     gene            3364..4524
                     /locus_tag="PLU99_12065"
     CDS             3364..4524
                     /locus_tag="PLU99_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO17829.1"
                     /translation="MNRFLPSLSPAGAQWARLFGLLLAAGLLAAAVVSLRPVLTPLLA
                     ALALAYILNPLVTWFEKRGCGRLLTIAAIAFLGVFALLTLGTYLVSLTIEQAAWLRDS
                     ANAYLERAAANPASGPAEHTARVMGWLNELAPTLKKDGLTIANAVFSFSAGLITDAFN
                     WLSLFVLLPMYTFFFLWRFNDMVRVVRDHLPAAARADIVYIVSTTDRAIASFFRGRLI
                     ICFLIGLTTGLGWTLTGVPFGLLLGGLAGLLTLVPFLSILALPPALLASYFVHAEGNW
                     VVPVTLAAGVFAFVQVLDSFVFSPVVTSKTSGLHPLTTVVALLIGGQWAGMLGLILAI
                     PVAGTLKALALTYVMPHIVRLAAAPAACEKEALPLEADIAAKENPIEVKAGR"
     gene            4551..4706
                     /locus_tag="PLU99_12070"
     CDS             4551..4706
                     /locus_tag="PLU99_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17830.1"
                     /translation="MAETTKIEPDSNRPYGTETRRSRYPLILLIVLFVCWFAALIVLA
                     VREAALR"
     gene            4703..>6353
                     /locus_tag="PLU99_12075"
     CDS             4703..>6353
                     /locus_tag="PLU99_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17831.1"
                     /translation="MTGADRLSLAGLDQAPVVELDPSIWEHNLGALRLQSPQFAAELE
                     QVALPSHWRCVRALDGSLTFRTEEPAQDPAWLGGTAAPLARAEGLLGSLVIGEQNAAL
                     PMLAAGAELRFLLDRLPPHRAVYVFENDLRTVAAVLRCADFARDIERGRVIFVPPADE
                     PGFLEHQLSAHPGLLPPGLIICLPQVSQSRLEQVRTICANCASAVSAARARRLQELAT
                     APPPTLAATCPPRLAVLALQLDPAAQDVARALEDAARKLDWPLLLDIVAGPQDVHPLV
                     HAERLASFEPNLIVSVGHSFDLPLRARATVCEWHLRSDQVGKAAANSALKLGASPHVS
                     DLLRRECPAGTRVFDWYWGCQPCDPTAPPKDVVLLVGDLPDDDPARAGIEQPSHVLLW
                     NSAQRCATQLWSKGELISASIVLKRAESAAGLSLPPGETRAALLRAIERTLIPATLLR
                     TILQALSSGAFGFGTIGHGWTPRAKSEREAIAFCSIWDFMSQAKPYRPIAAVFLGMDD
                     PLSPALVQAGALGWPLLLYGSGPAALSARLSPIFRAADYRTF"
BASE COUNT         1061 a         2042 c         2023 g         1227 t
ORIGIN      
        1 cttcaaatgg gaaatgcggt gagctcctgg caactcggcg ccgaaggaac cgaaatcgat
       61 tacggttctg cctgggacgc cgcggccttg tctgaactgc gccaggacat ccagaccttt
      121 gtcgctttcc cgcagatcgt cgtgcccaag tccgtttttg gccccgctgt ggaccaggac
      181 gctcccggta ccgggcagcg gcgccgtgag acggctgagg tggtttcgat ttacgtcccg
      241 ccgcatgcac cgactcgcgg cctgccggtg cagctcgcgc ggcacgcttc ggcaggcgct
      301 ccttggatca tgctggagac cgacgacgct ttgcctgttg aatctcggat cgtcgagtgc
      361 gcacgacgcc tgacgctcgc gaaggccgcg ggcgccgaac gcgtcttcct gccggccccc
      421 cttgaccgcg atcctgcgat gggacgttgg tgtccaaatg aactctacgt tccgctgcgc
      481 acgctgtgcc acttccttgg aggcaagcag gctacggccg tgctaccggc cgcaaacgag
      541 gatacgctgg cgatcctctt caccgacggg caagaggccg gctgcgtcgt ggcctggagc
      601 tggtgcgacc gttccgggcc gccggtggct atctgcggtg gtgaaggagc cgaggtttgg
      661 gatctctggg gcaggcgaag gcccggcgga gcaggtgagc ggcttgtcgt cccgctcgag
      721 gccgcgccgg tcattattgc cccggtcgag gcggccgccg tggccgtgca ggccaacctg
      781 gccatcgaac caaacctgat cgacgtggac ggatcagacg cgcgcccgat cctgaagctg
      841 accaacccgt ttgccgagga gatggcgggc gtcatgacgg tgaaagtgcc gccgtactgg
      901 cgcgccgccg agtcagaatc ggctgtgcag ctcgctcctg ggcagtcgct ggagcacgcg
      961 ctcgatctgg ccctgccgcc gcgagaaatt gcgggcgaaa agcacattgg ggtccagctc
     1021 aaactgtcgc ttccgcgccc gatgacgttg acggtttcga ttcccgtgcg gctgggactg
     1081 agcggcgcca gcttcgaggc tgtggcactg tggcgcggcc ccgatctggt tgtagagcag
     1141 gtggtccgca acaccgggcg tacgacgttg gatgcggttt gctcctgcca agccgcgggg
     1201 cggtgccggc ttgaaggtgc gctgctcgga atcgctcccg aacagacaac aacgaaggtc
     1261 ttcgaatttc tcgatgctcg ggagttggcc ggaagctcgc tcctggttgg gctggagaag
     1321 ccgaacgggc gccgccttga aaccgtcgtg gccgtcccgc cttgagcgct gagcccgaag
     1381 ggccattggc tccgtggcac cttgatttgc gtattttata ggacgtgagt gcaaggacga
     1441 gggtgaggaa aagctcgcag atcgctcact ccagcccaga cagcaggggg gtggccgtcg
     1501 cgtcctgcca gcgggggttt cggaatttat tggaattctc ctgtcaacac cgttatcatt
     1561 aacgcggtgt aagagaacgt tatcgctttg tcagccttag cttacggcga tttttgttct
     1621 ccaggaaatc gcgatggttg cccagcaagt cccaccaaac gcagcggtgg cccgaaccga
     1681 tgacgagcgg ccggccaccg gaaacagctc agggaggccg agccccccga ccgcgctggt
     1741 gcgttatggg gcgatggcct gggtcggcga gttcacctgc cgtccgggag cgcgtacgaa
     1801 ctgccacatg ccggtcgtcg tgcaaacgga tcgcggaatc gagctgggtt acccgttgct
     1861 tttctgcaac agtaactgcc cgctcgccat accgaaggaa accatccgca attacatcca
     1921 gaatagcggg cccgagttct gccgcgccaa cgccggacgc gtgctgcgcg aagcgaccgc
     1981 ccaagatgtc gacgagcacc tgcgcctcaa tgcgcacccc aaagaggacc tggagctctg
     2041 tgcggatctt gcggccaatc tcgggctgga cataaaaatc gtggccgccg agcacctttt
     2101 gggcggcgaa cgcatcgtct tctattttcg ttccgaaggc cgggtcgatt ttcgtgaact
     2161 ggtgaaggaa ctcgcacacc gcttccacac ccgcatcgag atgcgccagg tgggagcacg
     2221 cgacgaggca cgccttctgg ccgattggga ggtctgcggc cgtgagtgct gctgcaagag
     2281 ctttttgaag aagctgcgcc cggtcaacat gaagatggcc aaactccaga aatcgaccct
     2341 cgatccgtcg aaggtctcgg gccgttgcgg gcggttgcgc tgctgcctgc gcttcgagca
     2401 cgaaggctac acggagttgg ccgccaagct gccgcgcaga ggctcgcgag tccgcacgcg
     2461 gatgggcacc ggtacggtga tcgatcacca agtgctcacg cagctcgtgc tgattcgcac
     2521 ggatgagctc aacgagtgcg cggtgccctt ggaagaaatc ctggaatgtg atttgccccg
     2581 acctcgggcg gaggcgccgg cggcggacga gcggggaaag cccgcgtcca ccgaagcgcc
     2641 ggcccgcagc agcgggcgtg aagcggaccg cgcagcgcag ccgcgccgcg cgccgggcaa
     2701 gccttccggt tcgcattcgc cgcgcacgcc gaccgaaccg ccgtccagcg gccgcgccgc
     2761 gcatgattcc ggagacgagc cggttgaaat cgaaacgaaa gattcagacg catcatccgc
     2821 tcagccacgc gttgtccgac gacgccgtgg cgggcggcgg cgcaaccgtc ggcggccggg
     2881 agccgaccgc gagccggcgc ctgagaacta atttgaggtt acggagcgag cccacacgca
     2941 gatacaacca tgaagaccga agcgacacaa cgcctgatcg agacgatccg ctcggacgga
     3001 cgctatccca tcgaagcgta tgagttcctg cacgacggac tcgagtacac cacgcgctcg
     3061 ctgtatgggc gggtaacgcc caacgagccg cagcacgtga ccgggcagga gctgtgcgaa
     3121 ggactgcgcg atctggcgcg cgagcgctgg ggcccgttgg cgggcctggt gctgcgtcat
     3181 tggaacatct atcgcacgcg tgatttcggc gagatggtgt atttcctcat cgacctcaac
     3241 ttgatgggac gccaagagag cgaccgtatc gaggacttcg acgactgctt cgatctgcgt
     3301 acggccttcg attcctaccg catacctctg gacgtttacg actagcgccc cacaatagag
     3361 ggcatgaacc gtttcctccc gtcgctgtcg ccggccggag cgcagtgggc gcgcctcttc
     3421 ggcctgctgc tcgccgccgg gttgctggct gcggctgtcg tctcgttgcg gcccgttttg
     3481 accccgcttc tggcagcgct ggcgctggcc tacattctta atccgctggt gacgtggttt
     3541 gaaaagcgcg gctgcggacg tctgctgacg atcgcggcta tcgctttttt gggcgtcttt
     3601 gcactgctca ccttgggcac ctatttggtt tccctgacga tcgagcaggc ggcctggttg
     3661 cgcgacagcg ctaacgccta cctggagcgc gccgctgcca acccggcgtc cggccccgcc
     3721 gaacataccg cgcgcgtcat ggggtggctg aatgagttgg cgcccactct gaagaaagac
     3781 ggccttacga ttgcgaacgc cgtgttctcg ttctcggcgg gactgatcac cgacgcgttc
     3841 aactggttgt ccctgttcgt gctcttgccg atgtacacct ttttctttct gtggcggttc
     3901 aatgacatgg tgcgcgtcgt ccgcgaccat ctgcctgccg ccgcgcgcgc cgacatcgtg
     3961 tacatcgtca gcaccaccga ccgcgccatc gccagctttt tccgcggccg gctgatcatc
     4021 tgcttcctga tcgggctaac cacgggtctg gggtggacgc tgaccggcgt gccgttcggt
     4081 ttactgctcg gggggctggc cggcctgctg acgctcgtgc cattcttatc gattttggcg
     4141 ctgccgccgg ctctgctggc aagttacttc gttcatgccg aaggcaactg ggttgtcccg
     4201 gtcacgctgg cggccggcgt gttcgcattt gtacaggtgc tggattcgtt cgtcttctcg
     4261 cccgttgtca cgagcaagac gtccgggctt caccccctca ctaccgtggt ggcactgctc
     4321 atcggcggac agtgggccgg aatgttgggt ttgattctgg ccattccggt tgccggcacg
     4381 ctcaaagcgc tggccctgac ctacgtcatg ccgcatatag tgcgtttggc cgccgcgccc
     4441 gccgcctgtg aaaaggaggc gctccccttg gaagcggaca tcgccgccaa agaaaatccg
     4501 atagaggtga aggccggacg ataatcgcta gtcccctcgc ggataccaaa atggcagaaa
     4561 ccaccaagat tgaacctgat tccaatcggc cctacggtac cgaaacgcgc cgctcacgct
     4621 atcccctgat ccttttgatc gtgctgttcg tgtgctggtt cgcggccctg atcgtgttgg
     4681 cggtacgcga ggccgcgctt cgatgaccgg tgccgaccgg ttgagtttgg ccggtctcga
     4741 tcaggcgccc gtcgtcgagc tcgacccttc catctgggag cacaacctcg gcgcactgag
     4801 gctccagtcg ccgcaattcg cagccgaact ggaacaggtc gccctaccat cgcactggcg
     4861 ctgcgttcgg gccctggacg gctcgctcac gttccgtact gaagagccgg cccaggatcc
     4921 ggcctggcta ggcggaaccg ccgcgccgct ggcccgggct gagggattgc tgggatcgct
     4981 cgttatcggc gagcagaacg cggccttgcc tatgctggct gccggggcgg agctacgctt
     5041 tctgctggac aggctgccgc cgcatcgcgc ggtctacgtg ttcgaaaacg acctgcgcac
     5101 ggtggccgcc gtcctgcgct gcgccgattt cgcccgcgac atcgagcgtg ggcgcgtcat
     5161 tttcgtgccg ccggctgacg agcccggctt ccttgagcac caactgtccg cgcatccggg
     5221 cctacttccg ccaggcctaa taatttgtct accccaggtg tctcaaagtc ggctcgagca
     5281 ggtccgaacc atttgtgcga actgtgccag tgccgtgagt gccgcccgtg cccgccgcct
     5341 tcaagagctg gcaaccgctc cgcctcctac gctggccgca acctgtcctc cccgcctggc
     5401 cgtgctggcc ctgcagcttg acccggcggc gcaggacgtc gcgcgcgcgc tcgaagacgc
     5461 cgcccgcaag cttgattggc ctctccttct cgacatcgtg gccgggccgc aggacgttca
     5521 cccgctggta catgccgagc gactcgcaag ttttgagccc aacctcatcg tttctgtcgg
     5581 ccattcattt gatttgccat tgcgagcgag ggccacagtc tgcgaatggc atctgcggag
     5641 cgatcaggtc ggcaaggcgg ctgccaactc ggcgctgaag ctcggcgcgt cgccccacgt
     5701 ttcggacctt ttgcgccgcg agtgccccgc cggaacacgc gttttcgact ggtactgggg
     5761 ctgtcaaccg tgcgatccaa ccgctccacc gaaagacgtt gtactcctgg tgggggattt
     5821 gcctgatgac gatccggccc gcgcgggcat cgaacagcct tcacacgtac tgctttggaa
     5881 cagcgctcag cgctgcgcca cgcaactatg gtcaaagggg gaacttatct cagcctccat
     5941 tgtgctgaag cgcgccgaaa gtgccgcggg tctaagcctg ccgccaggcg aaactcgcgc
     6001 cgcgctcctg cgggcgatcg aacgcacctt gattcccgcc acgctgctac gaacaattct
     6061 gcaggcgtta tcgtcaggag ctttcggttt tggcacgatt ggtcatgggt ggacaccgcg
     6121 ggccaaatca gagagagagg cgatcgcatt ttgcagcatc tgggatttca tgagtcaagc
     6181 caagccctac cggccgatag cagccgtctt tttgggcatg gacgacccgc tctcgcccgc
     6241 gctggtgcaa gcgggcgcgc tgggttggcc gctgttgctc tacggaagcg gtcccgcggc
     6301 gctctcagcc aggctgagcc cgatattccg cgccgccgat taccgcacct tca
//
LOCUS       DAOSYS010000208         6248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10590_length_6248_cov_4.730825, whole genome shotgun sequence.
ACCESSION   DAOSYS010000208 DAOSYS010000000
VERSION     DAOSYS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10590_length_6248_cov_4.730825"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2671
                     /locus_tag="PLU99_12080"
     CDS             <1..2671
                     /locus_tag="PLU99_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17832.1"
                     /translation="EPPAQPEVVNPPAEPVAEPVQAVPSGTPATPLPTGTEVQPVPTG
                     TAAQPLPAGTPVEPLPTGAPVPVVEPIPMGIPAGPLAPRRATPLEELRARDELLWQRA
                     VALSQELAAQARDAAARNDFNEARRLVEAAAERIEAARNYAEPVAKYIAQRQSLEHLR
                     AEIARAEQFYLTVQAERERQEIAERVIQRQAQIEALRCEKVQQLFNTACQLRRQQRFN
                     EAAEVLRQVLQIDPTNAQARYQLQIVDDYASLMVQKEWQQTLDIQLRDSLVKAQEALI
                     PWDVEVLYPRNWLELSARRSQTGVGIGSPEERELQRRLDEILPQFQFEDAPLGAVLKH
                     IQEGRELNMAVDWTDLSSNGVAADRPISLGLRELPVRTVLKEVLAQAGGDIPLSYAVS
                     DGVLRVATKSKLDREKSVVVYDVRDLLASTMRQNNATRLDPTAAVMADKSSGRSLHDA
                     RLFESSESVAYEPPAAPPEGSADATINQIMSIIRQTVAPDSWHESGSGGDASIRELNG
                     QLIVYQTSGAQAQVRGLLEQLRAQRAMQIAIEARFLDVVQNFLDEFGVDLDFVFNSGG
                     AGYDRVFTPTGAFFDPFTGAPVLMPRQFSRIGTTAVTPTFGDPIPQTSVIQPYGRAAF
                     VPPAGNHGGASDQMTPIVVQQNSLSLTDPANLSTQVPGSFATPNFQPAMRVFGSFLDN
                     LQVDFLIRATQANNRSSIVQAPRIVMNNASSTSIQIGRISQYVATINPTVAEGAALAQ
                     PVVNAAQSGSGMGLFAVISHDRRYVQVTVNQLGQFDAPTFTTFETTRASGNSPAVSTQ
                     LLDQGFAQVYTQVSIPDGGTVLLGGLKQVGEVEIEAGVPILSKVPVMNRFFTNRSTVK
                     DTRTLLILMKAKIIIQDEAEEEAFPAFSAACPD"
     gene            2826..3773
                     /locus_tag="PLU99_12085"
     CDS             2826..3773
                     /locus_tag="PLU99_12085"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HOO17833.1"
                     /translation="MLSTALAGSSGQRPASEGGLPARRDSIGASSSRAAGPAPLAPGV
                     SIDWGQPAVLVDSEVVLRRGPVEFFACGRGKEHESILRLNASATHIYMALGLIGLNPG
                     HPPVWDDAIGDYGRPAGDLLDITCEWEEDGQSRSADAFEWLREIEFSRRPISRPWVFA
                     GSLRLADGTLAAEQSGAVFAVVDFPDSLVGQTGRHSSSNPELWLEAATDRIPPLGTPV
                     RLVLRPARPREYKVEVDFRGEAFVEGHYVAPAQLADLLLLARQVEPGRVQSLNLIGVL
                     RADEERLRRELAAAGCAEDFVRFQSQPPRAVGTTETGGD"
     gene            complement(3775..4200)
                     /locus_tag="PLU99_12090"
     CDS             complement(3775..4200)
                     /locus_tag="PLU99_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17834.1"
                     /translation="MAKILIADDNPQILELLEAYLEPLAAQIVLSADGQAALEAAQRE
                     HPDLILLDIMMPKRSGFEVCRLLKDDPRYRDIPIIMVTALNEFGDIERARECGAEDFL
                     CKPVSKVQLLERVQNLLKLAKNRGKRPGRGRTPNANQPE"
     gene            4370..5479
                     /locus_tag="PLU99_12095"
     CDS             4370..5479
                     /locus_tag="PLU99_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17835.1"
                     /translation="MHAPSRSNNRLTAVIALVLMVGLTSGLISCGSGGTGWESVTGPY
                     PRVDGSTGGTDTGPGNTGTTSGGVQGAGSRSSSDPCTETLNRKFVTISMRNFARDYIH
                     YFFIAIAFVDVDETDSSVFVPGLDNRAFPDGAVCPDDIGLYTQQGYQFVGSGDVREVG
                     DYCIVGPALYYFHQNGNFRVAAGTSDAGLGSGIAPAQGQLPSFDNFFTSAGAQLPVPD
                     LIIFHNPGAGRGATLKVSTPVPQPCSFAGVLGSEGGAPPCSRDSFYYVTFDDIMVGSR
                     ALGIDSGRRVPSDIQGTGCECAGSSEAYQLLAPSGARASSAQCNEFFRGGRIDYAFFQ
                     DDTTPAFPQLVWRVTDSTGAVAHQFDDLSPLAGSN"
     gene            complement(5622..>6248)
                     /locus_tag="PLU99_12100"
     CDS             complement(5622..>6248)
                     /locus_tag="PLU99_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17836.1"
                     /translation="AVDPTNVASLSTLIEMYLEAEQVEKALERARALVESARQARAAN
                     LPDRANLEQLITAQQTKIKCLSACYGLMCKRDARGRPTEQLESGKEADAVRILGEIAT
                     AQEEIAQLNWELGYHLTLQVIEPVARYQTEEIRERLETNTSHLLNLAGLYWATHQTDR
                     AVELFRRVVSLQKPSDPNPDEVRRNQETARQFLEQHGFPLTAPPEPQS"
BASE COUNT         1043 a         1815 c         2162 g         1228 t
ORIGIN      
        1 ggaaccaccg gcgcagccgg aggttgtgaa tccgcccgcg gagccggttg ctgagccggt
       61 gcaagcggtg ccttccggga ctccggcgac gccgttgccc acgggcacgg aggtgcagcc
      121 ggtaccgacg gggacggcgg cgcagccgct tccggctggg acgccggtag agccgttgcc
      181 gacgggggcg ccggttccgg tggtcgagcc gattccgatg gggattccgg cgggtccgtt
      241 ggcgccgcgg cgggccactc cgcttgaaga gttgcgggcg cgggacgagt tgttgtggca
      301 gcgtgcggta gccttgtcgc aggaactggc ggcgcaagcg cgtgacgccg cggctcgcaa
      361 tgacttcaac gaagcgcggc ggttggtgga agcggccgcg gagcgcatcg aagcagcccg
      421 taactacgcc gagccggtag ccaagtacat tgcgcagcgg cagtcgctgg agcacttgcg
      481 ggcggagatt gcgcgggcgg agcagtttta cctgacggtg caggcggaac gtgagcgtca
      541 ggagatcgca gagcgggtca tacagcgtca ggcgcagatt gaagctttgc gatgcgagaa
      601 ggttcagcag cttttcaaca cggcctgcca gttgcgccgg cagcaacgct tcaacgaggc
      661 ggccgaagtg ctgcggcagg tgttacagat tgatcccacg aacgcccagg cgcgttacca
      721 attgcagata gttgacgact acgcttcgct gatggtgcag aaggagtggc agcagacact
      781 cgacatccag ctgcgggatt cgctggtaaa ggctcaggaa gcgttaattc cgtgggacgt
      841 ggaggtgctc tatccgcgca actggctcga gctgagcgcg cggcgcagtc agacgggcgt
      901 gggcatcggc agtcccgaag agcgtgagct gcagcgccgc ctggacgaga ttctgccgca
      961 gttccagttc gaagatgccc cgctgggcgc cgtactgaaa cacattcagg aggggcgcga
     1021 gctcaacatg gcggtggatt ggacggatct gagcagcaac ggcgttgcgg ccgaccgtcc
     1081 gatttcactc ggcttgcgcg agctgccggt gcgcacggtg ttgaaagaag tgctggcgca
     1141 ggcgggcggc gacattccgc tctcgtacgc ggtttccgac ggcgtgctgc gggtggccac
     1201 caagagcaag ctcgaccgcg agaagtcggt ggtggtttac gacgtgcgcg acctgctggc
     1261 cagcacgatg cgtcagaaca acgcaacccg gctggatccg acagcggcgg tcatggccga
     1321 caaaagcagt gggcggtcgc tgcatgatgc gcgcttgttc gagtcttccg agagcgtggc
     1381 atacgagccg ccggcggcgc cccctgaggg ctcggcggac gcgaccatca accagattat
     1441 gagcatcatc cggcagacgg tggcgccgga ttcctggcac gaaagcggca gcggcggcga
     1501 cgcttcgatc cgcgagctga acggccagtt gatcgtgtat cagacgtccg gagcgcaggc
     1561 ccaggttcgc ggtctgctgg agcaactgcg ggcgcagcgc gccatgcaga ttgcgattga
     1621 agcccgcttc ctggatgtgg tgcagaactt cttggacgag tttggcgtcg acctggattt
     1681 cgtgttcaac tccgggggcg ccgggtacga tcgtgtgttc acgcccactg gagcgttctt
     1741 cgatccgttt accggcgcgc cggtgctgat gccgcggcag ttctcgcgaa ttggcacaac
     1801 ggcggtcacg ccaaccttcg gggatcccat accgcagact tcggtcatac agccgtatgg
     1861 gcgagccgcg ttcgttccgc cagccggaaa ccacggcgga gccagcgacc agatgacgcc
     1921 cattgtggtg caacagaatt cgctgtcgct gacggacccg gcgaacctgt cgacgcaggt
     1981 tccgggctcg tttgccacgc cgaattttca gcccgccatg cgagttttcg gctcgtttct
     2041 ggacaacctg caggtggatt tcctgatccg cgcgacgcag gccaacaatc gctcttccat
     2101 cgtgcaggcg ccgcgcatcg tgatgaataa cgcttccagc acgagcattc agatcgggcg
     2161 catcagccaa tacgtggcga cgattaaccc gaccgtggcc gaaggggcgg cgttggccca
     2221 gccggtggtc aatgcggccc aaagcgggtc gggcatgggg ttgttcgcgg tgatcagcca
     2281 cgatcggcgt tacgttcagg tgacggtcaa ccagttaggc cagtttgacg cgcccacgtt
     2341 cactacattc gagacgactc gggccagcgg taactcgccg gccgtttcga cgcagttgct
     2401 cgaccagggc tttgcccagg tctatacaca ggtttcgatt ccggacggcg gcacggttct
     2461 gctgggcggg ttgaaacaag taggcgaggt cgaaatcgag gccggcgtgc cgattttgag
     2521 caaagtgccg gtcatgaacc gcttcttcac caaccgcagc acggtgaagg acactcgcac
     2581 cctgttgatt ttgatgaaag ccaagatcat cattcaggac gaggccgaag aagaggcgtt
     2641 cccggccttc agcgccgctt gccctgattg aggaactggc gccgttgttt tgcagaaact
     2701 ccgatcagcg cccgccgctc accagcgacg ggcgctgtcg tttgttccga ccggtggtgc
     2761 aaacctccag ctttgctctg cgtgagcgtg gctcagccgc gtgcggccta ttgctggtgc
     2821 tgggcgtgct cagcaccgcg ctcgcgggat cgtcgggcca aaggcccgcc tcggagggcg
     2881 gcctcccagc gcgccgcgac tcaattggcg cctcgtcctc ccgagcggct ggtcccgcgc
     2941 cgctggcgcc cggggtaagc atcgattggg gtcaaccggc cgtgctggtg gattcggagg
     3001 ttgtgctgcg gcgcgggccg gtcgagttct tcgcctgcgg tcgcggcaag gaacacgaga
     3061 gcattttgcg cttgaacgca tcggccacgc acatttacat ggcgctgggc ctgatcggtc
     3121 tgaacccggg ccatccgcca gtttgggacg atgccatcgg cgactacgga cgtccggcgg
     3181 gcgatctgct cgacatcacg tgtgaatggg aagaggatgg ccagagccgg agcgccgacg
     3241 catttgagtg gctgcgcgag atcgagtttt cgcgccgccc catttcgcgg ccatgggttt
     3301 tcgccggctc gttgcggctg gccgatggca cgctggcggc tgagcagagc ggggccgttt
     3361 ttgcggtggt cgattttccc gacagcttgg taggtcagac gggccggcac tcgtcctcaa
     3421 acccggaact atggcttgag gcggcaaccg accgtattcc gccgcttggt acgccggtgc
     3481 gtttggttct gcgtccggcg cggccgcggg agtacaaggt ggaagtggat tttcgggggg
     3541 aggcgttcgt ggaggggcac tatgtagctc cggcgcaact ggcggatttg ttactgctgg
     3601 cgcggcaagt cgagccgggg cgagtgcagt ctttgaattt gattggcgtg ttgcgggcgg
     3661 acgaggagcg gctgcggcgc gaactggcag ccgccggttg cgcggaagac tttgtgcgat
     3721 ttcagtcgca gccgccgcgg gcggtgggca caacggagac gggcggggac tgaattattc
     3781 aggttgattg gcgtttggcg ttcggccgcg accggggcgc ttgccgcggt tcttggcgag
     3841 cttgagcagg ttctggacgc gttccagcag ttggaccttg ctgacgggct tgcagaggaa
     3901 gtcctcggca ccacactcgc gggcgcgctc gatgtcgccg aactcgttga gcgcagtgac
     3961 cataatgatc ggaatatcgc gataacgcgg atcgtccttc aacaagcggc aaacctcgaa
     4021 gccgctgcgc ttgggcatca tgatgtcgag caggattagg tcggggtgct cgcgctgggc
     4081 ggcctcgagg gcggcttgcc catcagcgct caagacgatc tgggcagcca gcggttcgag
     4141 gtatgcttcc agcaactcga ggatttgtgg gttgtcatcg gcgatcagga ttttggccac
     4201 gttgcctcca taactccgcg gcgttcatgc tcgcgccgcg caagggcagc ggagccgctt
     4261 gtcggaaacc tgggccgtca atatactgca acgttgcgtt ttcgccaccc ggaccgtcag
     4321 cggctcatgg agcggtctga ccttccgggg gcatccaagg gagttttaga tgcacgctcc
     4381 gtcgcgcagc aataaccggc ttaccgcggt gattgcgctg gttctgatgg tcggtttgac
     4441 gagtggcttg atcagttgcg gttcaggcgg aacgggctgg gaatcggtaa ccgggccgta
     4501 tccacgggta gacggctcta ccgggggcac ggatacgggg cccggcaata ccggcacgac
     4561 atccggcggg gtgcagggtg cgggtagccg gtcgtccagc gacccgtgta ccgagacact
     4621 gaaccggaaa ttcgtgacca tttcgatgcg caattttgcg cgtgactaca ttcactattt
     4681 tttcattgcg atcgcttttg tcgacgtgga tgagacggat agctccgtgt ttgtgccggg
     4741 gcttgacaac cgcgcgtttc cagatggcgc ggtttgcccg gacgacatcg ggctttacac
     4801 gcagcagggc tatcagttcg tcgggtcggg cgacgtgcga gaggtcggcg attactgcat
     4861 tgtggggccg gcgctttatt actttcacca aaacggcaac ttccgcgttg cggccggaac
     4921 gagtgatgcc gggttgggtt cagggatcgc gcccgcgcag gggcagttgc cgtcgttcga
     4981 caactttttc accagcgccg gcgcccagct tccggtgccc gacctgatca tcttccacaa
     5041 tcccggtgcg ggcagagggg cgacattgaa ggtcagtacc ccggttcctc agccatgctc
     5101 gttcgcgggc gttctgggtt cggagggtgg cgctcctcca tgcagtcgcg actcgttcta
     5161 ttacgtaacc tttgacgaca tcatggttgg ctcacgcgct ctgggtattg attcggggcg
     5221 gcgtgtacct tcggacattc aaggtaccgg ctgcgagtgt gccggcagct cggaagcgta
     5281 ccagcttttg gccccgtcgg gggcgcgagc gtcttcggcc caatgcaacg agtttttccg
     5341 cggcggccga atcgattacg cgttcttcca ggacgacacg acgccggcct tcccgcaact
     5401 tgtctggcgg gtcacagatt cgaccggcgc cgtagcccac caattcgacg acctctcccc
     5461 tctggcgggc agcaattaga ctggcggcag ttagaccggc agcaataaga ccagttccgc
     5521 gcgtccggcg cggattctgg cgtgtaccgg cggccggtcc tgaccggtgg gcgagttgcg
     5581 taggttaaca gaagccaggc gcggacgtga gctcgccagg ctcacgattg cggttcgggt
     5641 ggtgcggtca gcgggaagcc gtgctgctcg agaaactggc gggcggtctc ctggttgcgg
     5701 cgcacttcgt cggggtttgg atcgctcggt ttctgcaagc tgacaactcg cctgaagagt
     5761 tcgactgcgc ggtcggtttg gtgagtggcc cagtagaggc cggccaggtt caacaggtgg
     5821 ctggtgttgg tctccagccg ctcccgaatc tcctctgtct gataacgtgc cacgggttcg
     5881 attacttgca gagtcaaatg gtacccgagt tcccagttta gctgggcgat ctcctcttgg
     5941 gcggtcgcga tctcgccgag gatgcggacg gcgtcggcct ctttgccgct ttcgagctgc
     6001 tcagttggcc ggccgcgcgc gtcgcgcttg cacataagtc cgtagcaggc gctaagacac
     6061 ttgatcttgg tctgttgcgc ggtgatgagc tgctccaggt ttgcccggtc gggcagattg
     6121 gcggcgcgcg cctggcgggc ggactcgacc aaagcccggg cgcgctcgag cgccttttca
     6181 acttgctcgg cctccagata catctcgatc agggtggata aggaggccac gttggtgggg
     6241 tccacggc
//
LOCUS       DAOSYS010000209         6165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10743_length_6165_cov_5.291326, whole genome shotgun sequence.
ACCESSION   DAOSYS010000209 DAOSYS010000000
VERSION     DAOSYS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10743_length_6165_cov_5.291326"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="PLU99_12105"
     CDS             <1..1431
                     /locus_tag="PLU99_12105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO17837.1"
                     /translation="WGAVEALPVGEVLHGLPRYTDLTADSSWGAVWRDMRNDSHPPLY
                     FLLLHGWRRLVGDDELLVRLPSAIFSALSVIPVALILRALGRPRAGLVAALLLSVAYA
                     HVLIGQQARPYSLAVLLVSSSFALLIMLERRGAGLGRLQKTLCWSAYGVLVALAMLTH
                     YFAGLALLGQAVYVLFRLRGAALRNWAIALGCAGLVWALVWLPGFCAQWGFITGQDWV
                     ADAAPGRLPRTLIRALDLPVRMLLATRLPNWSSLSVIYPALLGLLLVVGSIVAVWRSR
                     TRVATVFLCWYLVPCIGLALLDALTSKQLLTHLRYSSVALPGLVGLLVLAASCLRRPM
                     PVVLAGIAAVAVALTLRLPALPSPQARVAAQFLQTQLRPGDLLVFDAQGWIPLWVRRD
                     LVQVTYYMPALACDLLMLNAAPPPETVARLGGYERIFVVSPRIDAVPHPAAGIMQLAA
                     RSEYIRDIGWIYSFARTAGQATTTQP"
     assembly_gap    1525..1624
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1964..2620
                     /locus_tag="PLU99_12110"
     CDS             1964..2620
                     /locus_tag="PLU99_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17838.1"
                     /translation="MKTTACVITLAVVALAVPVRGSIVSNHLILVQNGATVLMDHFDG
                     GTIDPMWTPVGSVGPIHDSVIDMDPGDGLLASLETQPDQTTIASTLFQIGAPFTDGYL
                     TLSLGGSDGSDFLALAAGDGSVALYDETGLRSLVPLALTPTLSLTLVCLPSGGTLALA
                     NEQVVFVGFSPVNDIASVFIGYTPIFEPAGAALLCFGLVVMAAARYAGRRGVGGGFGL
                     "
     gene            2947..3033
                     /locus_tag="PLU99_12115"
     tRNA            2947..3033
                     /locus_tag="PLU99_12115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2981..2983,aa:Ser,seq:gga)
     gene            3780..3914
                     /locus_tag="PLU99_12120"
     CDS             3780..3914
                     /locus_tag="PLU99_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17839.1"
                     /translation="MTWNWRLALVGAVAALLFIVDWGTYLIDWLFPERWPLIVGGVEY
                     "
     gene            4109..4411
                     /locus_tag="PLU99_12125"
     CDS             4109..4411
                     /locus_tag="PLU99_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17840.1"
                     /translation="MPEPTPSQSPDPQQQASGAPSQQPTPVQLKNAMKAFKKRLKLTR
                     LDDESRLGRGALSSGGRSGIVAITPPAQFPPAVWDELVRQGKLRRAGQGMYELTSE"
     gene            complement(4579..>6165)
                     /locus_tag="PLU99_12130"
     CDS             complement(4579..>6165)
                     /locus_tag="PLU99_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO17841.1"
                     /translation="SPHQAQATARLLQQGNVDLATAIQTAQQHVNGKAVNADAFVVPA
                     WQTGGTEQLKFAITCVTDNQLQTVFIDGQTGRVDHVRPAMTGRMGMGMGMGMGGFQPS
                     GYGQPQYGQPQQGQTQYGQPLTPYQQQYQQQPGYGQQPYGSWQERDWQQGQPQWQRWQ
                     QRGYGQPGQPGYWQSGRGWQGDAGWTPQGQFQPRYGAPQPWGTGAEFESQGYEAQHYQ
                     PGPYGQPMGGQYGQPMGGMSMMVMHPQRASQFIGDSVVDNQNQNIGRIEDLAVNLLTG
                     RVTFAILNADDKLYPIPLRLIQPRAADQAVLNISQNRLQSLPTLDKANWPQTINERLV
                     NAVHQSLGLQPEFGYAGFQAEGGPQPGVPQTQPAAPGGMMQPGAVPIRKATDLVNQSV
                     SNPQGENLGTIKDLVIDPQRARLAYVVVAFTNLGDKLLAVPWDAFTMQQGQIMLNIDQ
                     NRLQQAQHLTFTQDNWPDMTNPQWMQSAAEFWGTMPSPMMHGGRMMRPQHMRGPMQHG
                     PMPQRPGQQQMPPGQGGQQP"
BASE COUNT          980 a         1824 c         2002 g         1259 t
ORIGIN      
        1 tggggcgcgg tcgaggcgct gccggtgggg gaagtcctgc acggcctgcc gcgttacacg
       61 gatttgaccg cggactcctc gtggggcgcg gtttggcggg acatgcggaa cgacagccac
      121 ccgccattgt actttctgct gctgcatggg tggcgccgtc tcgtgggaga cgacgaactg
      181 ctggtgcgcc tgccgtcggc gattttttcg gcgttgtcgg tgattcccgt ggctctcatc
      241 ctgcgcgcgt tgggccggcc gcgggccggg ctggtcgcgg ccctgctcct gagcgtggcg
      301 tacgcgcacg tgctgatagg ccagcaggcg cggccgtaca gcttggcggt attgctcgtc
      361 agttcgagct tcgcgctgct gatcatgctc gaacggcgcg gcgcgggatt gggccggctg
      421 cagaagacgc tgtgctggtc ggcgtacggc gtgctggtcg cattggcgat gctgacgcac
      481 tacttcgccg ggctggcgct gctggggcag gcggtgtacg tgctgtttcg gctgcgtggg
      541 gcggcactgc ggaactgggc gatcgccctg gggtgtgccg gattggtttg ggcattggtc
      601 tggctgcctg gcttctgcgc ccagtggggg ttcataacgg ggcaggactg ggtggcggac
      661 gcggcgccag gacgcctgcc gagaactttg atccgggctc tggacctgcc cgtacgaatg
      721 ctgctggcca cgcggctgcc gaactggtcc tcgctcagcg ttatttaccc ggcgctgctc
      781 gggctgctac tggtggttgg ctcgatagtc gccgtgtggc ggtcgcgcac acgagtcgcg
      841 acagttttcc tatgttggta cctggttccc tgcatcggcc tggcgctgct ggatgcgctc
      901 accagcaaac agttgctcac acatctgcgc tactcatcag tcgcactgcc gggcctcgtg
      961 ggactgctgg tgctggcggc aagttgcctg cggcggccga tgccggtggt gctggcggga
     1021 attgcggcgg tcgcggtcgc gttgacttta aggctgccgg cgctgccgtc gccgcaagcg
     1081 cgcgtggcgg cgcagttcct gcaaacgcaa ttgcgcccgg gggatttgct ggtctttgac
     1141 gcccagggct ggattccgtt gtgggtgcgg cgggacctgg tgcaggtgac gtactacatg
     1201 ccggccctgg cgtgcgacct gttgatgttg aatgcagcac cgccgccgga aaccgtggcc
     1261 cggctgggcg gctatgagcg catctttgtg gtttcgccgc gaatcgacgc ggtaccgcat
     1321 cccgcggccg gaataatgca gttggcggcg cgctcagagt acattcgcga catcggctgg
     1381 atatattcct tcgcgcggac ggccggccag gcaacgacaa cacagccgtg agtgagcgtt
     1441 gttgtcacgg cgtgcgccag cgcgatcaca actccacaat gtcggaatgc ggaatgcagt
     1501 gattctcatg cggcaggggg gtagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnaactcc acaatgtcgg aatgcggaat gcagtgattc tcatgcggca ggggggtagt
     1681 attttcacgg gagcggcgcg tcgagttatt agtcgttagt cgttagtcct ggcgctcgcc
     1741 accttcccac gttcctcacc ttcccacctt cccacgcgtg attgtgggaa agtgggaagg
     1801 tgaggaatgt gggaaggtgc gcattttgtg cgagtagaaa agtggccacg cgcgtgcata
     1861 taacttcagc ggtggatgag taatgctcaa gcgcggtttc cggggggatc gcgctgaatg
     1921 cctgaaaact tctcggtttt ccgtactcag aaagaggaaa ccaatgaaga ctaccgcttg
     1981 tgtcattacc ttggcggttg tagccttggc ggtgccggtg cgcggcagca ttgtgagcaa
     2041 ccatctcata ctcgtgcaga atggtgctac ggtccttatg gaccatttcg acggcggcac
     2101 cattgaccca atgtggacgc cggtcggctc ggtcggcccg attcatgaca gcgtgattga
     2161 catggatccc ggcgacggtt tgctggccag cctcgagacg cagccggacc agaccacaat
     2221 cgccagcacg ctcttccaaa tcggcgcgcc gttcacggac ggttacctca cgctgagcct
     2281 gggtggatcg gacggctctg attttctggc gctcgcggcc ggcgacggat cggtcgcctt
     2341 gtacgacgaa accggtttgc gcagcctggt gccgttggca ttgacgccga cgctgtcgct
     2401 gacgctggtt tgcctcccga gcgggggaac gctggcgctg gcgaacgagc aggttgtgtt
     2461 tgtgggcttc agcccggtaa acgacatcgc gtcagttttc attgggtaca ctcccatctt
     2521 cgagccggcg ggcgcggcct tattgtgctt tgggctggtc gtaatggctg cggcgcggta
     2581 cgccgggcgc cgtggcgttg ggggcggttt cggtctgtag gattgcttgg cgccagcagc
     2641 ggcgcaggtc cgggcggtgg ggggcgcgcg cgtggcgtgc ggttcgcttg cgggagggat
     2701 cgtcggccga aggcgacggg gttaccggat tttttggaag ggtcctgagc tgccgttaaa
     2761 gcgcaagcgc ggccccggcg ccaaggcgtg ggggggtccg gtgctcgcgg cgtgcctggg
     2821 gcacgccctt ggggccgcgt cgcccggggc gggccgcgcc tccacccatc gtaaaaatgc
     2881 gcctcaagcg cggctctgtt gacgcactcc ccaccgtgct ttctactacg ccgtccggat
     2941 gccgctggag aggtgccgga gtggccgatc gggcacgctt ggaaagcgtg tgtacgggta
     3001 accgtaccgc gggttcgaat cccgccctct ccgattcaaa tactggaacc gcgcctctgc
     3061 gccggccatc gccccgttgg aaaccggtcc cacattcgac aaacaggcct gaaatcaggt
     3121 cgtattccca gcctgcttat tccgcagcct gcatgaggat ggacagaccc aaccagctgg
     3181 ccggcctgcc cgcagcgagg ccgccgcaaa ccgttatttg gatcggacct gcggcgctgt
     3241 gggtacaaag gggcaggcgc gtcggctgag ggcgccatgt acattgccgc tctgctggcg
     3301 tacgctggcc gctcaccgtc cagcggccga atggggcccg gagtttagga tgcgaccgtt
     3361 gcagagcggt tattattcta gagacgtcag aatatccagt attactggag tgaataggca
     3421 gggcttacta ccaaaggtct agccctaaag ctatacccca gtgtgtgcag ttgcattgtc
     3481 aggttgaatt ctttgtatac cgcgcgttcg gccttgacag ccggctgttc atgcgccaat
     3541 agttaatctg ttgttgctac agcagatgtg gcgcaatcgc agttgcaggt cgaataatgg
     3601 atgcaaccgg cagggacgct tgagctggtt atggcctttg ccgaacgaac ttgtgtcttc
     3661 acagcgcggg gcggtgttcc gtcacacctg ggcgccgcgc gagaatgctt tgcgattacc
     3721 gcgagtgtcg cgtgtccaga tctcatgtcc gctggaccag caaacaaaag gagaagcaca
     3781 tgacgtggaa ctggcgactg gctttggtag gggcggtggc ggctctcttg ttcattgtcg
     3841 actggggcac ttacctgatt gactggctct ttccggagcg atggccgctg attgtgggcg
     3901 gcgtggaata ttagttcgcc cccggttcgc accttttggc gcctttacgc ccgacgagca
     3961 ggcgcacccc cggagtcgtt ctgaaaaaca gggccgtgcg cgcggcttct cccgaagcgc
     4021 ggccgcggcc cgtaatcacc tggacgaagc tgatctctgt cggctatcct acgggagagc
     4081 aacacctgtg tcccgaagga tggctgacat gcctgaaccg acgccctcgc aatcacccga
     4141 cccgcagcag caagcgtccg gcgcgccctc gcagcagccg acgccggtac agcttaaaaa
     4201 cgcgatgaag gcgttcaaga aacggctcaa attgacccgc ctggatgacg agtcgcgtct
     4261 cggccgcggg gccctgagca gcgggggccg ctccggcatc gtcgccatta ccccgcccgc
     4321 gcagtttccc ccggcagtct gggatgagct ggtgcgccaa ggcaagctgc gccgtgccgg
     4381 ccagggcatg tacgaattga cctccgagta aacagccgcc ggatggcggc ctcgcagctt
     4441 cacaagccgg gccgctcctg tgcggccccg tgcacagccc tgaccactgc caaaaataga
     4501 ctaaaaaaaa acgagcgtga gcggcgcaga ccgtaccgcg cacactcacg cccaaacgca
     4561 cccgacgtct tacagatttt atggctgctg gccgccctgg cccggcggca tctgctgctg
     4621 gccaggtcgc tgcggcattg ggccgtgctg catgggcccg cgcatgtgtt gcggacgcat
     4681 cattcttccg ccgtgcatca tgggagacgg catcgtgccc cagaactcag cggcgctctg
     4741 catccactgc ggattagtca tatccggcca gttgtcctgg gtgaaagtca aatgctgcgc
     4801 ttgctgcagg cggttctgat cgatattcag cattatctgg ccctgctgca tggtgaaggc
     4861 gtcccacggc acagccaaca gcttgtcccc cagattagtg aacgcaacca ctacgtaggc
     4921 cagacgggcg cgctgcggat cgatcaccag gtctttaatc gtgcccaggt tctcgccctg
     4981 cggattgctc accgactggt tcaccaagtc tgttgctttg cgtatgggca cggctcccgg
     5041 ctgcatcatg ccgcccggcg ctgccggttg tgtctgcgga acacccggct gagggccgcc
     5101 ttcagcctgg aagcccgcat acccgaactc cggctgcaag cccagacttt ggtgtaccgc
     5161 attcaccagc cgctcattga tcgtctgcgg ccagttggct ttgtccaaag tcggcaaact
     5221 ctgcaggcgg ttctgcgaaa tgttaagaac cgcttgatcg gcggcgcgcg gctgaatcag
     5281 gcggagaggt attggataca gcttgtcatc tgcattgaga atcgcaaagg tcacccgccc
     5341 ggtcagcagg ttgacagcca gatcctctat gcgcccgata ttctgattct gattatcaac
     5401 tacactgtcg ccaatgaact ggctggcccg ctgcgggtgc atcaccatca ttgacatgcc
     5461 gcccattggc tggccgtact ggccgcccat cggctgaccg tatgggcccg gctggtaatg
     5521 ctgggcctcg taaccttgcg actcgaattc cgctcccgtg ccccacggct gcggcgcccc
     5581 gtagcgcggc tggaactgcc cctgtggggt ccagccggcg tcaccctgcc agccgcggcc
     5641 gctctgccaa taacccggct gcccgggctg accgtagccg cgctgctgcc agcgttgcca
     5701 ctgtggctgg ccctgctgcc agtcgcgctc ctgccaactg ccgtacggtt gctggccgta
     5761 tccgggttgc tgctggtatt gctgctggta tggagtgagg ggctgcccgt attgggtttg
     5821 cccctgttgg ggttgcccgt actgcggttg cccgtagccc gatggctgga atccacccat
     5881 gcccatgccc atgcccatgc ccatgcggcc ggtcatcgcg ggtcggacgt gatccacgcg
     5941 cccggtttga ccatctataa atacagtttg aagctggttg tccgtgacgc aagtaatggc
     6001 gaatttaagc tgctctgtgc cgccggtctg ccaggccggc acgacaaagg cgtcggcatt
     6061 gacggccttt ccgttgacgt gctgctgggc agtctgaata gcggtggcga gatcaacgtt
     6121 gccctgctga agcagccgcg cggtagcttg cgcctggtgc ggact
//
LOCUS       DAOSYS010000210         6104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10893_length_6104_cov_6.556290, whole genome shotgun sequence.
ACCESSION   DAOSYS010000210 DAOSYS010000000
VERSION     DAOSYS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10893_length_6104_cov_6.556290"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..3957
                     /locus_tag="PLU99_12135"
     CDS             184..3957
                     /locus_tag="PLU99_12135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO17842.1"
                     /translation="MDNGPTDSAESVFHQALRLPRAQRGAYLESVCGNNRALRCEVES
                     LLAAHDAAGGLLETPAVAGLTQPLIVDVETLAWSFTGEARFIGPYRLIEKLGEGGFGV
                     VYRAEQTAPVRRQVAVKLIKPGMDTREVIARFESERQALALMNHPNVAHVFDAGATAD
                     GRPYFVMEYVKGVPITEHCDRHCLPLEERLRLFMQVCEAVQHAHQKGIIHRDLKPSNI
                     LVEYAGGAARPKIIDFGIAKATDQRLTEKTVFTQRGQLIGTPEYMSPEQAEGSAQDVD
                     TCSDIYSLGVVLYELLTGLLPFDARRLRAAGYAALQRAIREQEPPKPSTRLSQVQTDG
                     DTAAAARKRKSETRTLIRRLRGDLDWIVMKCLEKERGRRYATAQALGEDIRRHLANEP
                     VSAGPPSAAYVFRKFVRRNRAAVIAAACVLVALAAGVIGTAVGLVQANAARDAESVQK
                     KVAEANAAEAKRAADRAEAVLRFLQDGLTTVNLDQAGADRDLKMREWVEAVAERLAAG
                     DLHEQPELEAELRRNVGLVCQSLGAYAEASEHLRRTLELQRGLHPGGHADVARGMRDL
                     SALLVMDSKLAEAEQVARDALEMSRRLSGTASVEYADGLMTLARALYEQGRHPQAEPL
                     VREALELRRELLGSEHADVAAALTLLGTIIGDYYGAPSERERYMREALGIYRKTLGDG
                     HPRTTRCLELLAHSLGDQMRYREAEILLREALALSRQRYGPEHRAIVNELRHLANVLW
                     RQDRDDEAEAAYRTALEMSRKLMGDKGETPPCLRAMAEFLAAQGRDSEAEELYNEALN
                     MDCRQGRWPFATLEPLSHLLYMQGRGKEVAELWLEVIERARHQGSGAAFTRTAQYLER
                     GPTHWSAKYSCGDEVGVWQACLDICRENLPSGHAARVSLLRVIAACHFAAGAFGAAES
                     SALAAYDEGVSNPSPEARQVLELIRWLVRLYCRTGQSEQAVEWCADYENWLGRLESDA
                     AATMRLAAGDMYTELGRYDLAEQHLAAALSHRVSVYGERDPYALVLPARIGCIRLAQG
                     RLDEAETVFHQVLGKLQAVSNVALVRGYSDDLWKLACKLVRARIEQGELAAAETLARK
                     ALASIDRTQSRDAAFMKSLLGEALRQRGALVEAESLLLAAYAELRDRLGPAFQREAGA
                     VAAHLVGLYEAWHAAAPDQGHDAQAAKWRGELEAWQASTRPASLQAPTQPATQPSARR
                     PRHPPPGTPAHARAARRWWPGFPRCQRPIAVSALCLPVSDSESMASPISLCCR"
     gene            complement(3878..4744)
                     /locus_tag="PLU99_12140"
     CDS             complement(3878..4744)
                     /locus_tag="PLU99_12140"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17843.1"
                     /translation="MHGARRRRAAQRSSTYPNERESEGQVMNQRRTWVMALLCGVGVF
                     VLAGGCPMQTEPEPEPEKTAPTPTYQVEVAVAGQGTVILEPAGGTYEEGTVVTITAAP
                     AEGYVFQGWSGDASGEEPTVTLAVYGDKTVTATFIVDTMAGTYTGVAKATFGDTSWNP
                     KVEAVFDETNKLTSFTYHYFDGTSETMPFGDYSGWPRREGETREIVELEWRETIKCNS
                     IDATPTSFMYDAFGVLQDREEGYDYGGTRYSFNLSRDGNTITGTFIYNIEKLGWPSIP
                     SLTLEDIKLTRQ"
     gene            4913..5038
                     /locus_tag="PLU99_12145"
     CDS             4913..5038
                     /locus_tag="PLU99_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17844.1"
                     /translation="MNDLQPVLGADAAAPILDIDQALERLAEITERNGERKLRVI"
     gene            complement(5176..>6104)
                     /locus_tag="PLU99_12150"
     CDS             complement(5176..>6104)
                     /locus_tag="PLU99_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO17845.1"
                     /translation="AFKIAAFPMHYWCPDVFEGAPVEVTTWLSIASKAAGLGLLLRIV
                     TTFGSGFDQPQHLLQPLALAVGVLAAITCTIGNLAALRQESVKRILAYSSIAHAGYMM
                     MAAAIIVGGTTLAAEGVNIAVAAVISYLFVYVIMNVGAFGATALVAWHCGSDHLSAFT
                     GLGRRAPWLALPMAICLFSLVGLPPLGGFAAKWFLLVALGRSAAAQPWLWVLVGVAVL
                     NTAISLYYYVRIIRQMYLTDDAAQPKLRLPIGGLAIINACAIMLLLLGTWWFSGLGDR
                     GQKLGANLYEPIATRVQQPPMAQAQAGVPAQP"
BASE COUNT         1116 a         1852 c         2083 g         1053 t
ORIGIN      
        1 gtcgccagta ctcaacgcat cggttgccaa tttttagggg actgccgggg gggggggggg
       61 atattgcccg atacataaag gaacgttcgc gaggattggg ctagcctggg tggcgcggcc
      121 ctgctagaat gcttgaacta cactgtctgt aaccgcgata ttgttctgga cgggaaatgc
      181 tgcatggata acggtcccac ggactcggcc gaaagcgttt ttcaccaggc gctgcgactg
      241 ccgcgcgcgc agcgcggggc gtacctggag tccgtgtgcg gcaacaaccg ggcgctgcgc
      301 tgcgaagtcg agtcgttgct ggcggcgcac gatgcggccg gcggtttgct ggaaacgccg
      361 gccgtggcgg gactgaccca accgttgata gtagatgttg aaacgctggc ctggagtttc
      421 acgggcgaag cgcggttcat cgggccctac cggctgattg agaagctggg cgagggcggg
      481 tttggcgtag tctaccgcgc ggagcagacc gcacccgtgc gccgccaagt ggcggtgaag
      541 ctcatcaagc cgggcatgga tacccgcgag gtgattgccc gctttgaatc cgagcggcag
      601 gccttggcgc tgatgaatca cccgaatgtg gcacacgtgt tcgatgccgg cgcgacggcg
      661 gacgggcggc cgtacttcgt catggagtat gtcaaaggcg tgcccatcac cgaacactgc
      721 gaccggcact gccttccgct cgaagagcgc ttgcggctgt tcatgcaggt ttgcgaagcc
      781 gtgcagcatg cgcaccagaa ggggatcatt catcgcgatc tgaagcccag taatattctg
      841 gtggagtatg ccggcggcgc ggccaggcca aagatcattg acttcggcat tgccaaggcg
      901 acggatcagc ggctcacaga aaagacggtg ttcacacagc gcggccaact gatcggcaca
      961 ccggaataca tgagcccgga gcaggcggag ggctccgccc aggacgtcga cacttgcagt
     1021 gacatttact cgctgggtgt ggtcttgtac gaattgctta cgggactgct gccgtttgac
     1081 gcgcgccggt tgcgggcggc cggatatgcg gccctgcagc gcgcgattcg tgagcaggag
     1141 ccgcccaagc ccagtacgcg actgagccag gtgcagactg acggcgacac ggcggctgcg
     1201 gcccgcaagc gcaagtctga aacccgcacg ctgatccgcc ggctgcgcgg ggatcttgac
     1261 tggatcgtga tgaaatgcct ggaaaaggag cgcgggcggc gttacgccac cgcccaggcg
     1321 ctgggcgagg acattcggcg gcacctggcg aatgagcccg tgagcgccgg gccgccgagt
     1381 gcggcatatg tattccggaa attcgtgcgg cggaatcggg cggcggtcat agcggccgcg
     1441 tgcgtgctgg tagcgctggc ggccggggta atcgggacgg cggtcgggtt ggttcaggcg
     1501 aacgcagccc gcgacgcaga gagtgtgcag aaaaaggttg ccgaagccaa cgcggccgag
     1561 gcgaagcgcg ccgcggatcg ggccgaagcg gtgctgcgtt ttcttcagga tggcctgacg
     1621 acagtgaatc tggatcaggc cggcgctgat cgcgatctga agatgcgcga gtgggtggag
     1681 gcggtggccg agaggctggc ggccggtgac ctgcacgagc agccggaact ggaggccgaa
     1741 ctgcggagga atgtgggcct ggtgtgtcag tcgctgggcg cgtatgccga ggcgagtgag
     1801 cacttgcgtc gaactttgga gctgcagcgg gggttgcatc cgggcggaca cgccgacgtg
     1861 gcgcgcggta tgcgcgatct cagtgcattg ctggtgatgg attcgaagct ggccgaggcg
     1921 gaacaggtgg cgcgcgacgc cctggagatg agccgccggt tatcgggaac ggcgagtgtc
     1981 gagtacgccg acggcctgat gacgctggcg cgggcgctgt acgagcaggg gcggcacccg
     2041 caggccgagc cgctggtgcg ggaagcgctc gaactgcggc gcgaactgct cggcagtgaa
     2101 cacgcggatg tggcggcggc gctgacgctg ctcgggacga tcattgggga ctattacggc
     2161 gcaccgagcg agcgcgaacg ctacatgcgc gaagcgctgg gcatctaccg gaagaccctg
     2221 ggcgacggcc acccgcggac gacgcgctgc ctggagttac tggcccattc gctgggcgat
     2281 cagatgcgtt atcgcgaggc cgagattctg ctgcgcgagg cgctcgcgct ctcacggcag
     2341 cgctatgggc ctgagcatcg tgcaatcgtg aacgagttgc ggcacctggc aaacgtgctt
     2401 tggagacagg accgggacga cgaggccgag gccgcgtatc gcacggcgct ggagatgagc
     2461 cgcaagctca tgggcgacaa aggcgaaacg cctccgtgcc tgcgcgcgat ggcggagttc
     2521 ctcgccgcgc agggccgcga ttcggaggcc gaggagctgt acaacgaagc cctgaatatg
     2581 gactgccgcc aggggcggtg gccctttgcg acgctggagc ccctttcgca tctgctgtat
     2641 atgcagggcc gtggaaaaga ggtcgccgaa ctgtggttgg aggtaatcga gcgagcgcgc
     2701 catcagggaa gtggagccgc gttcacccgg actgctcagt accttgaacg agggccgacc
     2761 cactggtctg cgaaatacag ttgtggcgac gaggtgggtg tgtggcaggc gtgtctggat
     2821 atttgccggg aaaacctgcc gagcgggcac gctgcgcgcg tcagtctttt gcgagttatc
     2881 gcggcctgcc atttcgcggc cggtgctttc ggcgcggccg aatcctccgc gctggcggcc
     2941 tatgatgagg gggtgagcaa cccaagtccc gaggcacggc aggtgctgga gctgattcgc
     3001 tggctggtgc ggctgtactg cagaacggga caatccgagc aagctgtcga atggtgcgcc
     3061 gattacgaga actggctcgg ccggctggag tccgacgcgg cggcgactat gagactggct
     3121 gccggcgata tgtacacgga gctggggcgc tacgacctgg ccgagcagca cttagccgcc
     3181 gccttgtcgc accgcgtcag cgtgtacggg gagcgcgacc cgtatgcatt ggtcttgccc
     3241 gccagaatcg gctgcatacg cctggcccag ggccgcttgg acgaagccga aaccgtattc
     3301 caccaggtgc tcggtaagct ccaggcagtc tcgaacgtcg cgcttgtgcg cggctacagc
     3361 gacgatctat ggaaacttgc ctgcaagctg gtgcgggccc gcattgagca gggcgaactc
     3421 gcagccgcgg aaacgctggc ccgcaaggcg ctggccagca tcgaccgcac tcaaagccgg
     3481 gacgcggcct tcatgaagag cctgctgggc gaggccctga ggcagcgcgg ggcgctggtg
     3541 gaagccgagt cgctgctgct ggcggcgtat gccgaactgc gggacaggtt gggtcccgcc
     3601 tttcagcgcg aagcgggcgc ggtggccgcg catctggtcg gcttgtatga ggcttggcac
     3661 gcggccgcgc cggaccaggg tcatgacgcg caagcggcca agtggcgggg cgaactggag
     3721 gcgtggcagg cgagcaccag gccggcaagc cttcaggctc cgacgcaacc tgccacgcaa
     3781 ccctccgccc gtcgaccacg ccatccgccg ccggggacgc cagcgcacgc tcgggcggcc
     3841 cggcgctggt ggccggggtt tccgaggtgc cagcgcccta ttgccgtgtc agctttatgt
     3901 cttccagtgt cagactcgga atcgatggcc agcccaattt ctctatgttg tagataaacg
     3961 tgccagtaat cgtattcccg tctctactca ggttgaagct gtaccgagta cctccatagt
     4021 cgtagccctc ttcgcggtcc tggagcaccc caaacgcatc gtacatgaag ctggtgggcg
     4081 tagcgtcaat actattgcat ttaatagtct cccgccattc gagttctact atctctcgag
     4141 tctcaccctc cctgcgcggc catccgctgt agtcgccgaa tggcatagtc tcgctggtgc
     4201 cgtcgaagta atgatatgtg aaactggtca acttgttcgt ctcgtcaaac accgcttcga
     4261 ccttggggtt ccaggacgtg tcgccaaaag ttgcctttgc aacgccggtg tatgtccctg
     4321 ccatggtgtc cacgatgaac gtggcagtta cggttttgtc gccgtatacg gccaacgtca
     4381 ccgttggctc ctcgccgctc gcatctccgc tccagccctg aaagacatat ccctccgccg
     4441 gcgcggccgt aatggtgacg accgtccctt cctcatacgt gcctccggcc ggctccaata
     4501 tcaccgtgcc ctgaccggcg acggccactt ccacctgata agtgggagtg ggggccgtct
     4561 tctccggttc gggctcgggc tcagtctgca tcgggcagcc tccggcaaga acaaacacac
     4621 cgacgccgca aagcaacgcc ataacccacg ttcttcgttg attcatgact tgaccctcgc
     4681 tttctctctc gttcgggtat gtgctggagc gctgcgccgc ccgccgccgc cgtgcaccgt
     4741 gcacggccgt cgcagacagg caggcaagcg ctccattcag caatgaatcc gtcagatgcc
     4801 cccgaaaccc ctcaggacta attgagaatt tcccaaaaaa aaatgcgccc acaccgacga
     4861 actggagccg aatgcctgcg tcgccgccac tctggcgcgc aagtcttgtt ccatgaacga
     4921 cttacagccg gtgctcggcg ctgacgcggc cgcgccgatc ctcgatatcg atcaggcact
     4981 ggagcggttg gccgaaatca cggagcgcaa cggcgaacgc aagttgcgcg tgatctaatg
     5041 ccggtccttc gcggggctga gtacgcccga aacgggcaag gccctcgaga tttccgaggc
     5101 gagcgactgg agttgcgccc gcacgtggcg gtattgggac ctaccaagct aagtcagggg
     5161 gcgttgatta cgcacttacg gctgggccgg cacgccggcc tgtgcctgcg ccatgggcgg
     5221 ctgttgcacg cgggtggcga ttggttcgta caggtttgcg cccagcttct ggccccggtc
     5281 gcctagtccg ctgaaccacc acgtcccgag cagcaatagc atgatcgcgc aggcgttgat
     5341 aattgccagg ccgccgattg gcagccgcaa cttcggttgg gccgcgtcgt cggtcaggta
     5401 catctggcgg atgatccgca cgtagtagta cagactgatg gcggtattca gcaccgccac
     5461 tcccaccagg acccacagcc acggctgggc cgcggcgctg cgcccgagcg ccaccagcag
     5521 gaaccacttg gccgcaaaac cgcccagcgg cggcagtcca accagtgaga agaggcagat
     5581 cgccatgggc agcgccagcc acggcgcgcg ccgccccagg ccggtgaacg cggacaggtg
     5641 gtcgctgcca caatgccagg ccaccaaggc cgtcgcaccg aaagcgccga cgttcatgat
     5701 gacgtacacg aaaaggtagg aaatcacggc cgcgactgcg atgttcacgc cctcggcggc
     5761 cagggtcgtc ccgccgacga taatcgcggc ggccatcatc atgtagccgg cgtgtgcgat
     5821 cgaggagtat gccagtatgc gcttaacgct ctcctgccgc agcgccgcca gattgccgat
     5881 ggtgcaggtg atggccgcga ggacgcccac cgccagcgcc aacggctgca gcaaatgttg
     5941 cggctggtcg aagccgctgc cgaacgtagt aacgattcgc agcaacagcc ccaagcctgc
     6001 ggccttgctg gcgatgctga gccaggtggt gacctcgacc ggcgcgcctt cgaaaacgtc
     6061 cgggcaccag tagtgcatcg ggaaggcggc gatcttgaac gcga
//
LOCUS       DAOSYS010000211         6080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10951_length_6080_cov_5.262573, whole genome shotgun sequence.
ACCESSION   DAOSYS010000211 DAOSYS010000000
VERSION     DAOSYS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10951_length_6080_cov_5.262573"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PLU99_12155"
     CDS             complement(<1..423)
                     /locus_tag="PLU99_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO17846.1"
                     /translation="MLLDVADLRTHFLTPAGAVPAVAGVSFSVAKGRTLALVGESGCG
                     KTVTVLSLLRLLPEQRVQVGGRALFAGRDLLKLSPREMRSIRGNRIAMVFQEPMSSLN
                     PVLTVGEQTAEPLRIHRRVSRRAARQRVVELFGLAGLPQ"
     gene            complement(529..1191)
                     /locus_tag="PLU99_12160"
     CDS             complement(529..1191)
                     /locus_tag="PLU99_12160"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO17847.1"
                     /translation="MAGKREITNAVNILHRRYVKDDPERKASLERERVNAEVAQIIHD
                     LRTEAGLSQKGLADAIGTTQSVISRLEDADYNGHSLTMLNRIAKALNHRLTVTMTAAD
                     PEAGTLRYAFHVVLRGLRRAKGLRIDELARHVDVDADELRALERGAHTRPRARTIHKL
                     STFYDVPAERMLQLAGAVREVPAEVRERASRFAAQSESFTKLSSEERRVLDEFVKFLR
                     SA"
     gene            complement(1295..1558)
                     /locus_tag="PLU99_12165"
     CDS             complement(1295..1558)
                     /locus_tag="PLU99_12165"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOO17848.1"
                     /translation="MPETDVLLFAEEDGSAPLLAWLDDLPPKVRDKCIVRIERLAQLG
                     HELRRPEADLLRDGIRELRVRHQGVHYRCSISFTSGAQWLHTG"
     gene            complement(1678..>1917)
                     /locus_tag="PLU99_12170"
     CDS             complement(1678..>1917)
                     /locus_tag="PLU99_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17849.1"
                     /translation="PIAASVVLPFTPFPAMHALIDAQEAFNWAGSASKVTMETARATC
                     AIFAVISAAAYLALTYSIYKNMVRGFDMTVRRQTA"
     assembly_gap    1918..2017
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2018..3382)
                     /locus_tag="PLU99_12175"
     CDS             complement(<2018..3382)
                     /locus_tag="PLU99_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17850.1"
                     /translation="MGILRDIGAWLWHLLPGNPILVRVVAMGSKRVRHLWARLIYLSA
                     LFLVLLVMGSGMLQGGREASLAELAKNSTQTFFWVSLVQLFLMSFIAPIFCAGAITQE
                     KDANTYHILLTTPLSNAQIVLGSLFSRIFFVWVLLLSGLPIFCIMMIYGGVTTAEIFQ
                     SFGLAACTGLLTGSIAIAISFVKVGTRRTIFAFFAGIATYLLAVWALGLSNFGQLREA
                     PQGTFQSGIRMSWLAPLHPFLSLLVVTGKTPAPAPEDVYSFGWPWGWILARPQYAYML
                     LTALASVLMILVSMFFVRSGSRQGEIKLASRLRDVFSSDNSGERRRRPRRVWRNPIAW
                     REAATRASAGGRSVLKWLFIVGGIGAGFLLLVAHYSSWASLSADQPAVTRHWLTVLTW
                     IELAVILLVVTNTAASTLTREKETQTIELLLSTPLTSQYIVAGMLRGLVSFALPMIAV
                     PTSTL"
     gene            3634..4833
                     /locus_tag="PLU99_12180"
     CDS             3634..4833
                     /locus_tag="PLU99_12180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOO17851.1"
                     /translation="MGDDSYDLAVIGGGPAGSSAARRAAQLGLWVLLLEKQVMPGIKP
                     CGGAFSEAALRQLGFELPPSLINSQVRGARVHYRRHASSVRLERRIAVLVSREDFDAL
                     LLEMARRAGTVCETATAKSLTRQGQRVLITTGERVFSASAVIIAQGAAGNLIRHVRRP
                     DQRGACGLCMTSRIAVPQRDPFAQSPDLVDIHFGVVKRGYGWLFHHGRYYSVGIGGTL
                     PLRQPYDALRTFCTSLGIDPPPPNAVRGHLIPRGGVPRVLVGGRMLLAGDAAGFVDPF
                     CGEGLAYTIRSGQIAAEVVAQAIRHGDLSAANLRAYEQRCEAEFGRSLRWALRVANVM
                     HTCPWPFMRLLAGNRRVLRGYLESLLARSYDGFLLWLARQMPRELIFGLTSGGESSLA
                     RSSRILD"
     gene            4904..>6080
                     /locus_tag="PLU99_12185"
     CDS             4904..>6080
                     /locus_tag="PLU99_12185"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOO17852.1"
                     /translation="MSGIETKLRPATSARDWRVRLKLITDTMREMSRQSDPQEMVHAY
                     ASRLSGILQNDRMIGLSRRGLQFPEFRITRDTEWKDPINPWTERHRLPVLRGGLLAEL
                     IYGQEPRIIQNLEVPANDPAASYLAGQRSLMAVPLFDGGVAMDMVIFLRRKVRGFRHR
                     DLPEHVWLTNLFGRATQSLVLSQRLREACDSADRELQDVADMQRALLPAELPQIPTMD
                     LAVHYQTSRRAGGDYYDFFALPEGRWGILMADVSGHGTPAAVLMAITHTIAHTYPAAP
                     CPAGQLLTRLNRQLSEIYTARSSNFVTAFYGIYDPATRGLTYACAGHSPPRLKRCSND
                     NLIVLNQASGMPLGIVPEEEYREASITLMPGDQIVLYTDGITETFDQAGEMFGTEGLD
                     "
BASE COUNT         1123 a         1887 c         1907 g         1063 t
ORIGIN      
        1 ctgcggcaac ccggccaggc cgaacagctc aaccacgcgc tgccgcgccg cgcggcggct
       61 gacccgccga tgaatccgca gcggctcggc cgtctgctcg ccgacggtca gcaccggatt
      121 caggctgctc atcggctcct gaaagaccat ggcgatgcgg ttgccgcgga tcgagcgcat
      181 ttcgcgcggg ctcagcttga gcaggtcccg cccggcaaac agggcccggc cgccaacttg
      241 gacgcgttgc tccggcagca atcgcaacag cgagagcacg gtcaccgtct tgccgcaacc
      301 cgattcgccc accagcgcca gcgtgcgacc cttcgcgacc gagaacgaca cccccgccac
      361 ggccggcaca gcacccgccg gtgtgaggaa gtgcgtccgc aaatcggcga cgtcgagcaa
      421 catcgggttc cattttacag cgcgcgagct gcgaagtggg gcagggctac cgccagagcc
      481 gagttctttc tgaacgtgca ggcggccgtg aactcgcgaa agcgttcact atgctgaccg
      541 caggaacttc acaaattcat cgaggactct gcgttcctcg gacgacaatt tggtgaacga
      601 ctcggactgg gcggcgaagc gcgaagcccg ctcgcgaacc tcggccggaa cttcacggac
      661 ggcgccggcg agttgcagca tccgttctgc aggcacgtca taaaatgtgc tcagcttgtg
      721 gatggtcctg gcgcggggcc ttgtgtgggc cccacgttcc aaggcgcgga gttcatccgc
      781 atcgacatca acgtgtctcg caagttcgtc aattcgcagg cccttcgcac ggcgcagtcc
      841 gcgcagaacg acgtggaagg cgtaccgtaa ggttccggct tccgggtctg cggcggtcat
      901 agtgaccgtc aggcggtggt tcaacgcctt ggcgatgcga ttcaacatcg tcagcgagtg
      961 gccattgtaa tctgcatcct ccaatcgact aataacggat tgcgttgttc cgatggcatc
     1021 agcgagtcct ttttggctca gacctgcttc ggtacgcagg tcgtgaatga tttgagccac
     1081 ttctgcgttt accctctccc gttcaagcga ggccttccgt tctgggtcat ccttcacgta
     1141 cctgcggtga aggatgttga cggcgtttgt gatctccctt ttgcctgcca tattcattct
     1201 tctttatgtg tatgttgctg cggcgcctcg gtgaacgcgg cctttcggcg gagtgccaag
     1261 tctatgtctg actccggtac gcggtcctct ttcatcagcc cgtgtgtaac cactgcgcgc
     1321 cgctcgtgaa agaaatagag catcggtaat gaacgccctg gtggcgcacg cgcaattcac
     1381 gaatgccgtc cctaagcagg tccgcttcag gccgccgcag ctcgtgaccc aactgcgcca
     1441 acctctcgat ccggacgata cacttgtcgc ggaccttggg tggcaagtcg tccaaccaag
     1501 ccagcagcgg agcggaaccg tcctcttcgg cgaacagcag gacatctgtt tccggcatcc
     1561 ggctcacctg tcattagtat cgcattattg cgatcagaag tcaagccccc ttcatcgtaa
     1621 ttttgcgatt atgcgcaaca ggcgttcggc aacgcggaca gaattgccgc tccgccccta
     1681 cgccgtctgc cgccgcacgg tcatgtcgaa gccgcgcacc atattcttat agatgctgta
     1741 agtcagcgcc aggtaggccg cggcagagat cacggcgaag atcgcgcacg tcgcccgggc
     1801 cgtctccatc gtcaccttcg aagcggagcc cgcccaattg aatgcctcct gtgcgtcgat
     1861 cagggcgtgc atggcaggaa acggcgtgaa cggcagaacc acggacgcgg cgatcggnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgtactggtc ggcacggcga
     2041 tcatcggcag ggcgaagctg accaggccgc gcagcatgcc ggccacgatg tactgactcg
     2101 tcagcggcgt cgagagcagc agctcgatgg tctgcgtctc cttttcgcgg gtcagcgtgc
     2161 tggccgcggt gttggtcacg accagcagaa tgaccgccag ctcaatccac gtgagcacgg
     2221 tcagccagtg gcgtgtgacg gccggctgat ccgcggacag cgaggcccaa ctcgaatagt
     2281 gtgcgacaag cagcaggaaa ccggccccaa tgccgcccac gatgaacagc cacttcagga
     2341 ccgagcgtcc gccggccgag gcgcgcgtgg cggcctcccg ccaggcaatg gggttgcgcc
     2401 agacgcggcg tgggcggcgg cggcgctcgc cggaattatc ggaggaaaag acatcacgca
     2461 agcggctggc cagcttaatt tcaccctggc gcgacccgga ccgcacgaag aacatgctaa
     2521 ccaggatcat gagcaccgac gcgagcgcgg tcagcagcat gtacgcgtac tgcgggcgcg
     2581 ccagtatcca gccccacggc cagccgaagc tataaacgtc ttcaggcgca ggtgccggtg
     2641 tcttgccggt cacgaccagc agggagagaa acgggtgcag cggcgccagc cagctcatcc
     2701 tgattccgct ctggaacgtc ccctgcggcg cttcacgaag ctgcccgaaa ttcgacagcc
     2761 ccagcgccca gaccgccagc agatacgtgg cgattccggc gaaaaaggcg aagatcgtgc
     2821 ggcgcgtgcc cactttgacg aagctgattg cgatcgcaat cgaacccgtc agcagtccgg
     2881 tacacgccgc cagcccgaaa ctctggaaaa tttcggccgt cgtgactccg ccgtagatca
     2941 tcatgatgca gaaaatcggc aggccggaca gtaacaacac ccagacgaag aagatgcggc
     3001 tgaaaagtga accgagcacg atctgggcgt tgctgagcgg cgtggtcagc aaaatgtggt
     3061 aagtgttggc gtccttttcc tgcgtaatag cgcccgcgca gaagatcggc gcgatgaagc
     3121 tcatcaggaa gagctgcacc aggcttaccc agaagaaggt ctgggtcgag ttcttggcca
     3181 gctcggccag cgaggcttcg cggccgcctt gcagcatgcc gctgcccatt accagtagca
     3241 ccaggaagag cgccgagagg tagatcaggc gtgcccacaa atggcgcacg cgcttgctgc
     3301 ccatggcgac cacgcgcacc aggattggat ttcccggcag caagtgccag agccaggcgc
     3361 caatgtctcg cagaatgccc atcgcttgcc gttgctccat gtcggcgggg cggtcgcgcc
     3421 cgccggctta ctcatccgag ccggccgcat tgtaaccgcc cccacttcgg agtacggtgg
     3481 gggccgcgag aaggcacaaa gcccaaatgg gtaccgccca gcgtcgggcc gcagttgtcg
     3541 ggcatcgccg ttgagagggg gcctgcctgc cgtcgccaga cgccgcaccg ctccatacac
     3601 taatttggca atcgggcgca caggggcagc cgaatgggcg acgactcata cgatctggcc
     3661 gtgatcgggg gcgggccggc cggcagcagc gctgcccggc gtgcggccca gctcgggcta
     3721 tgggtcctgt tgctggagaa gcaggtcatg cccgggataa agccgtgcgg cggggcgttt
     3781 tcggaggccg ccctgcgaca gctcggcttt gaactaccgc cgtcgctgat aaacagccag
     3841 gtccgcggcg cacgcgtgca ctatcgcagg catgcttcgt cagtgcggct ggagcggcgc
     3901 atcgctgtgc tggtgagccg cgaggatttc gatgcgctgc tgctggaaat ggcgcggcgc
     3961 gccgggacag tttgcgagac tgccactgcg aaaagtctga ctcgtcaagg ccagcgcgtg
     4021 ctcatcacga ccggcgagcg cgtcttttcc gcctcggccg tcataatcgc ccagggcgcg
     4081 gccggaaact tgatccgcca cgtccgccgc cccgaccagc gcggcgcctg cggactgtgc
     4141 atgaccagcc gcattgcggt tccacagcga gatccgttcg cccaatcgcc ggacctggtg
     4201 gacattcact tcggtgtcgt aaagcgcggc tacggctggc tgtttcatca cgggcggtac
     4261 tactcggttg gcatcggcgg aacacttccc ctgcggcagc catacgacgc acttcggact
     4321 ttttgcacca gcctgggaat agacccgccg cctccgaacg cggtgcgcgg gcatttgatc
     4381 ccgcgcggtg gcgtgccgcg cgtgctggtg ggcgggcgga tgttgttggc gggcgatgcg
     4441 gccggtttcg tcgatccctt ttgcggagaa gggctggcgt acacgatccg ctcaggacag
     4501 attgcggccg aagtcgtggc gcaggcgatc cgccacggcg acttgagcgc cgcaaatctt
     4561 cgggcctacg agcagcgctg cgaggcggag ttcggccgta gtctgcggtg ggcgttgcgg
     4621 gtggcgaatg tgatgcacac atgtccgtgg ccgttcatgc ggctgctggc cggcaaccgg
     4681 cgcgtgctgc ggggctattt ggagtcgctg ctggcgcgct cttacgacgg attcctactg
     4741 tggctggcgc gtcagatgcc ccgtgagctg atctttgggc tgaccagcgg cggcgagagc
     4801 agcttggcga gaagctcgcg cattctggac taattgggcg ggcggggacg gcaaggctat
     4861 actaggtcag accgaaacga tccttactat cgggaaagga ccgatgagtg gcatcgaaac
     4921 taagctgcgc ccagcgacaa gcgctcgcga ctggcgtgtc cggctgaagt tgattaccga
     4981 cacgatgcgt gaaatgagcc ggcaaagcga cccgcaggag atggtccacg cttacgcctc
     5041 gcgattgtcg ggcattctgc agaacgatcg gatgatcggc ctcagccggc gcgggcttca
     5101 gtttcccgag tttcggataa cgcgagatac ggagtggaaa gacccaatta atccatggac
     5161 ggagcgccac cgtttgcccg tgctgcgcgg cgggctgctg gccgagctga tctacggcca
     5221 ggagccgcgc atcatccaga acctcgaagt cccggcaaac gacccggcgg cctcatacct
     5281 cgccggccag cgctcactca tggctgtgcc gctgtttgac ggcggggtgg cgatggacat
     5341 ggtgatcttc ctgcgacgca aggtgcgcgg cttccgacac cgcgatctcc cggagcacgt
     5401 gtggctgact aacctgttcg ggcgcgctac gcaaagcctg gtgctgtcgc aacggctgcg
     5461 ggaggcatgc gacagtgcgg atcgcgagct tcaggatgta gccgacatgc agcgggcgct
     5521 gttgccggcc gagttgccac aaattccgac catggactta gcggttcatt accagacctc
     5581 gcgccgggcg ggcggcgatt attacgactt tttcgctctg cccgagggca gatggggcat
     5641 cttgatggcc gacgtcagcg ggcacggcac gccggccgcc gtgctgatgg caatcaccca
     5701 caccatcgcc cacacttacc cggccgcgcc atgccccgcc ggccagttac tgacacggct
     5761 caatcggcag ctcagcgaaa tatacacggc gcgctccagc aacttcgtaa cggcttttta
     5821 cggcatttac gaccctgcaa cgcgcggctt gacgtacgcc tgcgccggcc atagcccgcc
     5881 gcgtctgaag cgctgctcca acgacaacct gatcgtgctc aaccaggcca gcggcatgcc
     5941 gctgggcatc gtaccggaag aggaataccg cgaggcaagc atcacgctta tgccgggcga
     6001 tcaaattgtc ctctacaccg atgggataac ggagacgttt gatcaggcgg gggagatgtt
     6061 tggcaccgag gggctggatc
//
LOCUS       DAOSYS010000212         6063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10989_length_6063_cov_5.619008, whole genome shotgun sequence.
ACCESSION   DAOSYS010000212 DAOSYS010000000
VERSION     DAOSYS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10989_length_6063_cov_5.619008"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..2512)
                     /locus_tag="PLU99_12190"
     CDS             complement(284..2512)
                     /locus_tag="PLU99_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOO17853.1"
                     /translation="MRALTLWLAAGVILGAAGAWAAAGEPVRFDGERVVRVMVHSPQQ
                     LAQLLSMTDDVWTEGATSGPIDVRMTPEQFAQLQASGLKFVVLIEDVQAAIEAERTPP
                     ERGGFFDHYWSLADNIAYLESLVALRPDLAQMFTAGTTLEGRGIRGIRITAPGPGTKP
                     GVLVHGAQHAREWITVPVVMWTADKLIREYDTDPYIRSLVDNLEWHLVPVMNPDGYTY
                     CWTTYRMWRKNRRYDAATGCYGVDCNRNWGFGWGGPGASSSPCSEEYCGPAPFSELET
                     QAMRDWILTHPNIRAYVDLHSYSQIIFYPYSFGDVWPPEPDRSDYQYAVEHMAEMVHD
                     VHGLSYDYGRGLDVVYQVSGASRDWVYGSAGRYAIGTELRDTGAYGFLLPENQIIPQC
                     EEYVPALMWYADYMATPQRLTSPNGGETLTSGTTATITWYTHGGTPTSVDLYLSVDGG
                     VTFPYTIATGEAPDGSYSWFVTETLSTHCRVKLVVHRADLTSGEDVSDADFTIVGGPQ
                     RVYSFPLDTDPGWTTEGQWAWGDPAGSGGDPQVGYTGNNVYGYNLAGHYTNNMPRYNL
                     TTTALNLSNVTGSQLRFRRWLGVQNANYDHAGIEISTDGVTWNSVWEHTGASINDASW
                     TLQAYDISAFADQQSTVYIRWAMGTTNASVTYCGWNIDDVEIWGVVTQPQWAIGDVNC
                     DGDVNVFDIDPFVLALTDEPGYAAQYPACDINNADINGDGAINVFDIDPFVLCLTGGG
                     CG"
     gene            3061..>6063
                     /gene="nifJ"
                     /locus_tag="PLU99_12195"
     CDS             3061..>6063
                     /gene="nifJ"
                     /locus_tag="PLU99_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOO17854.1"
                     /translation="MSNRRMVTIDGNQAAAHVAHKVSEVIAIYPITPSSPMGEWADEW
                     SAAGQPNLWGTIPHVVEMQSEGGASAAVHGAASTGALTCTFTASQGLLLMIPTMFKMA
                     GELLPTVFHVTARTLATHALSIFGDHSDVMACRSTGFAMLCSGSVQEVTDMALIAHAA
                     TLESRVPFVHFFDGFRTSHEVNKIEELTREDMLAMLDETALRAHRERALTPDRPKLRG
                     SAQNPDVFFQARETVNPFYNRCPQVVQKVMDRFAKVVGRQYHLFDYVGAPDADRVIVM
                     MGSGAEAAHETVDYLNAHGAKVGLVKVRLYRPFVASELFKVLPKTVKAIAVLDRTKEP
                     GAAGEPLYKDVVAGLGECVAAGNLPLPSMPRVVGGRYGLSSKEFSPAMVKGVYDNLAA
                     AQPKNYFTIGIKEDVTGSSLDYDPKFVTEDKDVVRALFFGLGSDGTVGANKNSIKIIG
                     EDTDNYAQGYFVYDSKKAGSMTASHLRFGPKPIRSSYLITSANFVACHNWAFLERFDM
                     LSTLNPGGVFLLNSPFPKEETWDRLPVEVQQQIIDKKLKVYAIDAYAVAKATGMGGRV
                     NTIMQTCFFAISGVLPREQAIEAIKKSIKKEYSRKGEEVVKKNWAAVDQTIANMYEIP
                     VPAKATSKLRMRPAVPEEAPDFVKEVLGPMIVFKGDELPTSKLPCDGTFPIATTRWEK
                     RNIALEIPVWNPDLCIQCGKCAIVCPHAAIRMKAYDAKLLASAPASFRHKKYIGREFE
                     GYETTVQVAPEDCTGCALCIEVCPAKDKQEPKHKAINMEFQPPIRERERENYDFFLSL
                     PETDRSKLNIKSVKGSQFLQPLFEYSGACAGCGETPYIKLITQLFGDRAVMSNATGCS
                     SIYGGNLPTAPWAMNPQGRGPAWCNSLFEDNAEFGFGYRLTIDKHAEFARELVGKLRE
                     QIGADLADGLLNAVQKTDADIDLQRKRVTALKAKLAQVNLPEARQLLDLADMLVKKSV
                     WIIGGDGWAYDIGYGGLDHVLACGRNVNI"
BASE COUNT         1170 a         2057 c         1788 g         1048 t
ORIGIN      
        1 ccggccttat cgagcgcccg gtgcggatcg cgcagggcgc gctcgtccca gacgcgcggc
       61 tgatcgcttt gaggctgctg cgaatcgaac atcgcgaatg ctcccgctgg ccggttcgaa
      121 ccgtgaattg taacagcgcc ggccgcgcgc agcgccaccc ggcggctcac aggtcctgcc
      181 tcagcccaat cgtcgcgtgt gaaggagcgg cagcgccgat gaaagggctg ccatcgaggt
      241 tgtcgtcgcc cgtgtgccgc ccacgcgtgc gggcgaatcc ggctcaaccg cacccgccgc
      301 cggtcaggca cagcacgaac ggatcaatgt cgaagacatt aatcgcgccg tcgccattaa
      361 tatccgcgtt gttgatgtcg cacgccggat attgggcggc atacccaggc tcgtccgtca
      421 acgccagcac gaacgggtca atgtcaaaga cgttcacgtc gccgtcgcag ttcacgtcac
      481 caatagccca ctgcggctgc gtgaccacgc cccagatttc gacgtcgtcg atgttccacc
      541 cgcagtacgt gacgcttgcg ttggtcgttc ccatggccca gcggatgtaa acggtgctct
      601 gctggtcggc gaacgccgaa atatcgtagg cctgcagcgt ccagctcgca tcgttgatgc
      661 tggctccggt gtgctcccag acgctgttcc acgtaacgcc gtcggttgaa atttcgatcc
      721 cggcgtggtc gtagttcgca ttctgcacac ccagccagcg cctgaatcgc agttgcgagc
      781 cggtcacgtt agacagattg agggcggtcg tcgtgaggtt atagcgtggc atgttattgg
      841 tgtaatggcc ggccaggttg taaccataga cgttattacc cgtatacccc acctgcgggt
      901 caccgccgct accggccggg tcgccccagg cccactgccc ttcggtagtc cagcccggat
      961 cggtgtcgag cggaaaactg tagactctct gcggaccgcc gacaatagtg aagtcggcat
     1021 cggatacgtc ttctccggac gtcagatccg cccggtgcac taccagcttc acgcgacaat
     1081 gggtggacag tgtttccgta acgaaccagg aatacgagcc gtcgggcgct tcgccggtgg
     1141 cgatcgtata tgggaaggtc acgccgccat caaccgacaa atacaaatcg acgctggtgg
     1201 gcgtgccgcc gtgcgtatac caggtgatag tcgcggtcgt cccggaggtc agcgtctcgc
     1261 cgccattggg cgaggtcaga cgttgcggtg tcgccatgta gtcggcgtac cacatcaatg
     1321 ccggtacata ttcttcgcac tgcggaatga tctggttctc cggcaacaga aaaccataag
     1381 ctcccgtgtc gcgcaactcc gtgccaatgg cataccgccc ggcgctgccg tagacccaat
     1441 cgcgcgaggc gccgctgacc tggtatacca cgtccaggcc gcgcccgtag tcatacgaca
     1501 gaccatgcac atcgtgaacc atttccgcca tgtgctccac agcgtactga tagtcgctac
     1561 ggtccggctc cggcggccag acatcgccga aactgtaggg gtagaaaatg atctgcgaat
     1621 aagagtgcaa atcaacgtat gccctgatat tcggatgcgt caaaatccag tcgcgcatcg
     1681 cctgggtttc cagttccgag aagggcgccg ggccgcaata ctcttcagag catgggctgg
     1741 agctggcgcc cggcccgccc cagccgaagc cccagttgcg gttgcagtcg acgccgtagc
     1801 agccggtggc cgcatcgtag cgccggttct tgcgccacat acggtaagtg gtccagcaat
     1861 aggtgtaccc gtctggattc attacgggaa ccaggtgcca ttccagattg tcaaccagcg
     1921 agcgaatgta cgggtcggtg tcgtattcac gaatcagctt gtccgccgtc cacatcacca
     1981 ctggtacggt gatccactcg cgcgcgtgct gcgccccatg caccagcacg ccgggcttcg
     2041 tcccaggtcc cggcgcagtt atacgtatgc cccgaatgcc ccggccttcc agcgtcgttc
     2101 cggccgtgaa catctgggcc aaatccggcc gcaatgcaac cagcgactcc aaataggcaa
     2161 tgttgtccgc caggctccag tagtgatcga agaacccgcc gcgctcaggg ggcgttcgct
     2221 ccgcctcgat ggcggcctgc acgtcttcaa tcaagaccac gaatttgagg ccggaagcct
     2281 gcaattgggc gaactgctcg ggcgtcattc gcacgtcgat cggcccgctg gttgcgccct
     2341 ccgtccagac gtcgtccgtc atgctcaaga gctgcgcaag ctgctgcgga ctgtgtacca
     2401 tgacgcggac tacccgctcg ccgtcaaagc gcactggctc gccggccgcc gcccacgccc
     2461 ccgctgcgcc tagaatcacc ccggccgcca accatagagt aagtgctctc acaacccact
     2521 ccttacgcga aagggcttaa gtccagccga aacccttcgg tcagcctgta tactaccatg
     2581 cgacccctgc cgcgcaaggg ccattttcga cacccggcgc cgcctcacca gcgccggtct
     2641 acttggcttc cctgtcggct tcacccttcc ctgcccgggc cgcgtgcctc tttcaaccgc
     2701 cgccggttca ggatcgttga acccccgcaa gagctgacgc caatgtcaga tacttggacc
     2761 caacgaatcc gaatccgccg ccttatggca gtgccaccgc cgcaacatcc tctccgtgcc
     2821 cctaagcacg cacgcccctg cgcagaaatc cccagcggtt tgcggagacg ccggcccgcc
     2881 cggcttgcag cacccacccc atttcggtta aaacggcacg ctaattgctg ccggaaaccg
     2941 ttccggtgcg cctaggggcc tcggcaggtc accccgaacg cggcccaaag ccccaaaaag
     3001 cgtcctgaga gcagccggct gaaccggcca aacgcgaatt ttttcttgga gtcagaaccc
     3061 atgagtaaca gacgcatggt gacgattgac gggaatcagg ccgcggccca cgtggcccac
     3121 aaggtcagcg aggtgatcgc gatctacccg atcaccccct cttcgcccat gggcgagtgg
     3181 gccgacgagt ggtccgccgc cgggcaaccc aacctgtggg gcacgatccc gcacgtggtc
     3241 gagatgcaga gcgaaggcgg ggccagcgcc gcggtccacg gggccgcctc caccggcgcg
     3301 ctgacctgca cttttaccgc ctcccagggc ctgctgctga tgatcccgac catgttcaag
     3361 atggccggcg aactgctgcc gaccgtcttc cacgttacgg cccgcacgct ggccacgcat
     3421 gccctctcga tcttcggcga tcacagcgac gtgatggcct gccgttcgac cggcttcgcc
     3481 atgctgtgct ccgggtcggt gcaggaagtg acggacatgg ccctgatcgc gcacgccgcc
     3541 acgctcgagt cgcgcgtgcc gttcgtgcac ttcttcgacg gcttccgcac ctcgcacgag
     3601 gtcaacaaaa tcgaagagct gacgcgcgag gatatgctgg ccatgctgga cgagaccgcc
     3661 ctacgggccc accgcgagcg tgccctcacg cccgaccgcc cgaagctgag aggctcggcc
     3721 cagaacccgg acgtgttctt ccaggcccgc gagacggtca acccgttcta caaccgctgc
     3781 ccgcaagtcg tgcagaaggt catggaccgc ttcgccaagg tcgtcggccg gcagtatcac
     3841 ctgttcgatt acgtcggggc tccggacgcc gatcgcgtaa tcgtcatgat gggctcgggc
     3901 gccgaggcgg cccacgagac cgtcgattac ctgaacgccc acggcgcgaa ggtcggcctg
     3961 gtcaaagtcc gcctgtaccg cccgttcgtg gcttccgagc tgttcaaagt cctgcccaag
     4021 accgtcaaag cgatcgcggt tctcgaccgt accaaggagc cgggcgccgc gggcgagccg
     4081 ctctacaagg acgtcgtggc cggcctgggc gagtgtgtcg cggctggaaa tctgccgctg
     4141 cccagcatgc cgcgcgtggt tggcgggcgt tacggcctgt ccagcaagga attctcgccg
     4201 gccatggtca agggcgtgta cgacaacctg gcggccgccc agcccaagaa ctacttcacg
     4261 atcggcatta aggaagacgt caccggttcc agcctggatt acgacccgaa attcgtgacc
     4321 gaggacaagg acgtcgttcg ggccctgttc ttcggcctgg gctcggacgg cacggtcggc
     4381 gcgaacaaga actcgatcaa gatcatcggc gaggacacgg acaactacgc ccaaggctac
     4441 ttcgtctacg actcgaagaa ggccggctct atgacggcct cgcacctgcg tttcggcccg
     4501 aagccgatcc ggtccagcta cctcattacc tcggccaact tcgtcgcctg ccacaactgg
     4561 gccttcctcg agcgcttcga catgctctcg acgctgaacc cgggcggcgt cttcctgctc
     4621 aacagcccgt tccccaaaga ggaaacgtgg gatcgtctgc ccgtcgaagt ccagcagcag
     4681 atcatcgaca agaagctgaa ggtctacgcg attgacgcct acgccgtggc caaggccacc
     4741 ggcatgggcg gccgcgtgaa caccatcatg cagacctgct tcttcgccat ctccggcgtg
     4801 ctgccgcgcg agcaggccat cgaggccatc aagaaatcca tcaagaagga atacagccgc
     4861 aagggcgagg aagtcgtcaa gaagaactgg gccgcggtcg atcagaccat cgccaacatg
     4921 tacgaaatcc ccgtgccggc caaggccacc agcaagctcc gcatgcggcc ggccgtgccc
     4981 gaggaagcgc ccgacttcgt gaaggaagtc ctcggcccga tgattgtctt caagggcgac
     5041 gagctgccga ccagcaagct gccgtgcgac ggcacgttcc cgatcgcgac cacgcgttgg
     5101 gaaaagcgca acatcgccct ggaaatcccg gtctggaacc cggacctgtg catccagtgc
     5161 ggcaagtgcg cgatcgtttg cccgcacgcc gccatccgca tgaaggccta cgacgccaag
     5221 ctcctagcca gcgccccggc cagcttcagg cacaagaagt acatcggccg cgagttcgag
     5281 ggctacgaga cgaccgtcca ggtcgcgccc gaggactgca ccggctgcgc cctgtgcatt
     5341 gaggtctgcc cggccaagga caagcaggag cccaagcaca aagccattaa catggagttc
     5401 cagccgccga tccgcgagcg cgaacgcgag aattacgact tcttcctctc gctgcctgag
     5461 acggatcgca gcaagctgaa catcaagagc gtcaagggct cgcagttcct gcagccgctg
     5521 ttcgagtatt ccggcgcctg tgccggctgc ggcgaaacgc catatatcaa gctgatcacc
     5581 cagcttttcg gcgaccgcgc cgtcatgtcg aacgcgaccg gctgctcctc gatctacggc
     5641 ggcaacctgc ccaccgcgcc ctgggccatg aacccgcagg gccgcggacc ggcctggtgc
     5701 aactcgctat tcgaggacaa cgccgagttc ggcttcggct accggctgac catcgacaag
     5761 cacgctgagt tcgcccgcga gctggtcggc aagctgcgcg agcagatcgg ggctgacctc
     5821 gcggacggcc tgctcaacgc cgtacagaaa acggatgccg acatcgacct gcagcgcaaa
     5881 cgcgtcacag cgctgaaggc caagctggcc caggtgaact tgcccgaggc tcgccaactg
     5941 ctcgatttgg ccgacatgct cgtcaagaag agcgtctgga tcatcggcgg cgacggctgg
     6001 gcgtacgaca tcggctacgg cggcctggat cacgttttgg cctgcggccg caacgtaaac
     6061 atc
//
LOCUS       DAOSYS010000213         6062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_10992_length_6062_cov_5.100216, whole genome shotgun sequence.
ACCESSION   DAOSYS010000213 DAOSYS010000000
VERSION     DAOSYS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10992_length_6062_cov_5.100216"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLU99_12200"
     CDS             complement(<1..615)
                     /locus_tag="PLU99_12200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO17855.1"
                     /translation="MADSRHPHSPTSPGSSPGATRSAAADQATAAAVNGGDSLPQAEL
                     NTPATHPDDPTRASSDALKGIVDLEGARIAGYQLLEELHRGGQGVVYKAIQLGTKRCV
                     ALKVMLEGPFASEATRRRFEREVELAASLRHPYIVTILDSGVSAGRFYFAMEYIDGLR
                     LDRYLAKSRPSLRETLTLFEKICAAVNYAHQRGVIHRDLKPSNIL"
     gene            807..1940
                     /locus_tag="PLU99_12205"
     CDS             807..1940
                     /locus_tag="PLU99_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17856.1"
                     /translation="MRFGSLVHYSTLFALLISFSICEAAAPAAQETQSTSSVSPAEHE
                     ARWFRGVRQMTSTEMGLDRAGEAYFSPDGKRICFQAYPKGQSEYQIYVMNVDGTGLKM
                     VSTGQGATTCSFFHPSNEKMIFASNHLDQRPPKMPEEIEKVVKRVGARRYTWPFYPGM
                     DVYEYTFATGQLRPLITGPDYDAECAYSPDGKLIVFCSSRDDNLDIYICDADDGKNLR
                     RITTAPGYDGGPFFSPDGKRVIYRSNRRDIDSGTMQIFTNNLEGTDERALTDHEILHW
                     CPFYHPSGKWVIYTRGDHSDPRHPSYDLYLVREDGSESHRVTADPAFDGLPVFSPDGR
                     YMMWTSKRGVDAPQIFMAEFIGLTPEGELRAEPVEKPSEQSRD"
     gene            2055..2972
                     /locus_tag="PLU99_12210"
     CDS             2055..2972
                     /locus_tag="PLU99_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO17857.1"
                     /translation="MFKVLLAAPLDATSEARLEAGAEVVRAPAPDEQTLCRLIGDCDA
                     LVARTETRVTRTVLSAGRKLRVVGVAGVGLENVDAHAAEELGIAVLNVPAAASDAVAE
                     FAVALMLQLLRPVPRLASAYRGGDFRAARQTPHGRELRELTVGIVGMGRIGSRVGRIC
                     AAGCGARVLYNDIIDVGPLAYPAQAVDKPTIWSQSDIVTLHVPLTDQTRGLVNAEVLA
                     QFRPTAFLVNTARGAIVDTGALTAALQTGRLGGAALDVTFPEPLPPDDPLFACDNCIL
                     TPHVAARTIGGLQRMFGVVDDVLAYLQIA"
     assembly_gap    3487..3586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3587..5888
                     /locus_tag="PLU99_12215"
     CDS             <3587..5888
                     /locus_tag="PLU99_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17858.1"
                     /translation="ADRHLFAPTRKLPAPLGRAAEHLAILVNSTLYPGIRDSLSQYAA
                     DLASEGYTVYVETVAGGAPEDIKDWIQERYAFGCTGVVFIGDIAAAWAEVSGDVFPSD
                     LFYMDLDGHWQDADHDGDYEIHEAGIGDEGPELYVARLYAHTLDYGAEADLINDYLAK
                     AHAYWTGTLTQPWGGLEYVDKDWFDMEVNLDQIYGANVVRHDYGHYTTAADYLDQMDL
                     GQHFVQVCAHSYSGAHHFSTAPTESASYAHVYVYSPTTRDAYLLLGSDDGIKAWLNTE
                     LVCTHDVYQEWAPDQFFHAVTLNEGWNQLLCKISQEGGDYRFSARFTDPLLATMPDLV
                     YQLSDPDTHSDEPQFIRRWLVNGFHQDDPDNFWSYLTTSYLGGPEYDVNPSAGQVMGG
                     QIWTEYFTDGAYVDLDLYGGELDYGVTYAFARVYAKTPIVCQLWLGYDDGARVWLNGD
                     EIVYDSRYGDFTPDMKKVYVDLLSGENRLLVKVSEWAGRHGFSARFAYADGSAVSGLT
                     YDPEPEPISYICTWLMNGPYVNPDLGTRLSQDYLGDEAGVRPSVGEVAAQGSWEAGAS
                     GIPFDIAAFYDRDGGWVLSETIQQRDPPVLFYNLFACGPGRFTDDNYLAGAYIFNTTY
                     GLITVASAKSGSMLNFVDFTTPLGQGCTIGEAYRQWFEVQAPFELWEREWYYGMVLNG
                     DPTLRPVRKGDLDRDGRIDLDDYAALCDCLAGPDTPPSPDQPGLTIEVCLRAFDFDSD
                     EDVDTVDFEAFQWSFTGSYEKGSSPP"
BASE COUNT         1176 a         1962 c         1751 g         1073 t
ORIGIN      
        1 gaggatgttg ctcggcttga ggtcgcggtg gatcacgccg cgctggtggg cgtaattcac
       61 tgccgcgcag atcttctcga acagcgtcag cgtctcacgc aggctgggcc gcgacttggc
      121 caggtaccgg tcgaggcgca gcccgtcgat gtactccatg gcgaagtaga accggcctgc
      181 gcttacaccg gagtcgagga tggtgacgat gtaggggtgt cgcagggacg cggccagttc
      241 gacctcgcgc tcgaagcgcc gccgggtggc ctcgctcgcg aaaggcccct cgagcatcac
      301 cttcaaagcc acgcagcgtt tcgtgccaag ctggatcgcc ttatagacca cgccctggcc
      361 gccgcggtgc agttcttcga ggagctgata gccggcgatt cgagcgcctt ccaggtccac
      421 aatgcctttg agcgcgtcgc tcgaagcccg cgtcgggtca tccggatgcg tcgctggcgt
      481 gttgagttca gcctgcggta acgagtcgcc gccatttacg gccgcagcag tcgcctgatc
      541 cgcggccgca gagcgggtcg cccccggtga cgagccgggc gaggtcggag aatgtggatg
      601 gcgcgaatca gccataacac ccaatgttac atgggtttgt aaatgccgaa ctccgaaaat
      661 cctaccgctt cacgtatgca acctcagacg gccagcttaa ttgactcttg tcgaaaaggc
      721 gcttacataa gcgcgcgaat attcgaaccg tgggcggtgt tcgtgggccg gatacgccac
      781 gcttcgcgat cacaaaggag gacaacgtgc ggtttggttc gttggttcac tacagcaccc
      841 tgtttgccct gctaatctct ttttccattt gcgaagccgc ggcgccagcc gcacaagaga
      901 cgcagagcac ttcgtctgtc tcgcccgccg agcacgaggc acgctggttc cgtggcgtgc
      961 ggcagatgac gtccaccgaa atggggctgg accgcgccgg cgaggcgtat ttctcgccgg
     1021 atgggaagcg aatctgcttc caggcctatc ccaaaggcca atccgagtac caaatctacg
     1081 tcatgaacgt ggacggtacg ggactgaaaa tggtcagcac cggccaaggc gcaactacct
     1141 gctcgttctt ccatcccagc aacgaaaaga tgattttcgc gtcgaatcac cttgaccagc
     1201 ggccgccaaa aatgccggag gaaatcgaaa aagtcgtcaa acgagtcggc gcccgcaggt
     1261 acacttggcc tttctatccc ggcatggacg tatatgaata cacgttcgcg accggacagt
     1321 tgcggccgct tattaccgga cctgattacg acgccgaatg tgcctattcg cccgatggga
     1381 aattgatcgt cttctgctcc agccgcgacg acaacttgga catctacatt tgcgacgccg
     1441 acgacgggaa aaatctgcgc cgcattacga ccgccccagg ctatgacggc gggccgttct
     1501 tcagccccga tggcaaacgc gtcatttacc gttcaaaccg gcgggacatc gatagcggca
     1561 caatgcaaat cttcaccaac aacttggagg gcaccgacga acgcgccctc accgaccatg
     1621 aaattctcca ctggtgcccg ttttatcatc cttccggaaa atgggtcatc tatacccgcg
     1681 gcgaccattc ggacccgcgc catccgagct acgacctcta ccttgtccgc gaggacggct
     1741 cagagagcca tcgcgtaacc gcggatcccg cttttgacgg cttgcccgtg ttctcccccg
     1801 acgggcggta catgatgtgg acctcaaaac gcggcgtcga cgcgccgcag attttcatgg
     1861 cggaattcat cggcctgacc ccggaaggcg aactgcgggc ggagccggtc gaaaagccat
     1921 ccgaacagag ccgcgactag ttcctgacga accccgcggc gcgggagggc gaagcttctg
     1981 ctgagcgcag ctcggtcgcc ctggcgattt gcgagtggga gcctcgcccc cccgaaacac
     2041 gctgccgatt tgcgatgttc aaagtcttac ttgcagcacc gcttgatgcg acctccgaag
     2101 cccggctgga agccggcgca gaggtggtcc gcgcgcctgc tcccgatgag cagaccctct
     2161 gccggctgat cggcgactgc gatgccctcg tggcccgcac ggaaacgcgt gtcacccgca
     2221 ccgtcctttc agccggccga aagctgcgcg tggtcggcgt ggccggcgta gggttggaaa
     2281 acgtcgacgc gcacgcggcc gaagagctcg gcatcgccgt gctgaacgtt cccgccgccg
     2341 cctcggacgc ggtcgccgag ttcgccgtcg ccctcatgtt gcaactcctg cgaccggtgc
     2401 cccggctggc aagcgcttat cgggggggtg atttccgggc cgcgcggcaa accccgcatg
     2461 gccgcgagct gcgcgagctc acggtaggca tcgtgggtat gggccgaatc ggctcgcgcg
     2521 tcgggcggat ttgtgcggcc ggttgcgggg cacgcgtact gtacaacgac atcattgacg
     2581 tcggtccgct ggcttacccg gcgcaggccg tggacaaacc gacgatctgg tcgcagagcg
     2641 acatcgtgac tctgcacgtt ccgctcaccg atcaaacgcg cgggctggtg aacgccgaag
     2701 tgctggctca gttccgcccc accgcgttcc tggtcaacac cgcccggggc gccatagtag
     2761 acacgggcgc cctgaccgcc gccctgcaga ccggccgtct gggcggcgcg gcactggatg
     2821 tgacatttcc agaaccgctc ccgcccgatg accccctctt tgcttgcgac aactgcattc
     2881 tcacgccaca cgtcgccgcc cgaaccatcg gcggcttgca gcgcatgttc ggcgtagtag
     2941 acgatgtact ggcgtatctg caaatagcgt gacacaaatc cacgcttatc cgaccaatcc
     3001 ggaacgaaag ctgcggccag aggatacgtc gtgtctgcgg ccactagagt ccgttacaca
     3061 ccgcgaaata agtcaacaat ttgcacttgc gtttgcggcc ggcgagccga tttcgatata
     3121 gtggaatcgt gaaaagcaat gaccggccgg agtatgggaa ggaagttcgc actgcgaaac
     3181 cggtccagcc acagccgcgc ggaaccggac acgccaaccc cgtcccccaa atggctgccc
     3241 aggaacccat cccccaatca gcctcacagg agcccatcca atgaaacacg cacggtgctt
     3301 ctaccttgcc gtcatgctcg cagcgagcat tgctctcgac gcaacagccg acccaacggc
     3361 gccacaaagc tcagaccagc cggcgacgtc ggcagcctcc gcctcaacag gttccgctca
     3421 acgacaagac ggccgcttca tcccccgtct gcgctgggtg gcgcctcacg gcgaattgcc
     3481 cggcacnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgcg gatcggcatc
     3601 tcttcgcgcc gacacgcaaa ctgcccgccc cacttggccg cgccgccgaa cacctcgcga
     3661 tcctcgtcaa tagcacactt tatcccggca ttagagacag cctcagccaa tacgccgccg
     3721 acctcgcttc agaaggctac accgtctacg tcgaaacagt ggccggtgga gccccggaag
     3781 acatcaagga ctggattcag gagcgctacg cattcggctg caccggcgtc gtcttcatcg
     3841 gcgacatcgc cgccgcctgg gccgaagtgt ccggcgatgt cttccccagc gacttgttct
     3901 acatggacct ggacggccac tggcaggacg ccgaccacga cggcgactat gagatccatg
     3961 aggccggaat cggcgacgaa ggcccggagc tctacgttgc ccggctgtac gcacacacgc
     4021 tcgactacgg cgccgaggcc gacttgatca atgactacct cgccaaagcg cacgcttatt
     4081 ggacaggcac gctgacgcaa ccttggggcg gcctcgaata cgtcgacaag gactggtttg
     4141 acatggaggt caacctggac caaatctatg gcgccaatgt cgtgcggcac gactacggcc
     4201 attacacgac cgcggccgac tacctcgacc agatggacct cggccagcat tttgtccagg
     4261 tttgcgccca cagctactcc ggcgcccatc acttctcgac ggccccgacc gagtccgcat
     4321 cgtacgccca cgtgtacgtc tacagcccca cgacgcgcga cgcctacctg ctgctaggca
     4381 gcgacgacgg cataaaggcc tggctcaaca ccgaactggt ctgcacgcac gacgtgtacc
     4441 aggagtgggc ccccgaccag ttcttccacg ccgtcacgct caacgaaggc tggaaccagc
     4501 ttctgtgcaa gatcagccag gagggcggcg actaccggtt ctcggcccgc ttcactgatc
     4561 cgctgctggc cacgatgcct gacttggtct accaactcag cgaccccgac acccacagcg
     4621 atgagccgca attcatccgc cgctggctcg tcaacggctt tcatcaggac gatcccgaca
     4681 acttctggag ctatctgact acgagctatc tgggcggccc cgagtacgac gtcaacccaa
     4741 gcgccgggca ggtgatgggt ggccagatct ggaccgagta cttcaccgac ggagcatacg
     4801 tcgacctcga tctgtatgga ggtgaattag actacggcgt cacatacgcc tttgcccgag
     4861 tctacgcgaa aacaccaatc gtgtgccagc tttggctcgg ctacgatgac ggggcacgcg
     4921 tctggcttaa tggcgacgag atcgtgtatg actcccgcta cggcgacttc acgcccgaca
     4981 tgaaaaaggt ctacgttgac ctgctgtccg gcgaaaaccg cctgctggtc aaggtgtcgg
     5041 agtgggcggg ccgtcacggg ttcagcgccc gctttgcgta tgcagacggc agtgcagtct
     5101 ccgggctgac atatgatccg gagcccgagc ccatttccta catctgcacc tggctcatga
     5161 acggccctta cgtcaacccc gacctcggca cacgcctgag tcaggactat ctcggcgacg
     5221 aggccggcgt gcggcccagc gtgggcgaag tcgctgcaca ggggtcttgg gaagccggtg
     5281 cgagcggcat accgtttgac atcgccgcgt tctacgatcg cgacggcggc tgggtgctct
     5341 cggagacgat ccagcagcgc gacccgcccg tgctgttcta taacttgttc gcctgcggcc
     5401 cggggcgctt caccgatgac aactacctgg ccggcgccta catattcaat acgacttatg
     5461 gcctgatcac cgttgcctcc gcgaagagcg gcagcatgct caatttcgtg gactttacca
     5521 cgccgctcgg ccaaggttgc actatcggcg aggcttatcg ccagtggttc gaagtgcagg
     5581 ccccattcga gttgtgggag cgcgagtggt actacggcat ggtgctcaac ggcgacccca
     5641 cgctgcgtcc cgtccgcaag ggcgacctcg atcgcgatgg ccgtattgac ctggacgatt
     5701 acgccgccct atgcgactgc ctggccggtc cggacacgcc gccgtcacct gaccagcccg
     5761 gcctgacgat tgaggtgtgc cttcgggcct ttgatttcga cagcgacgaa gacgtcgaca
     5821 cagtcgactt tgaagccttc cagtggagct tcactggttc gtatgagaaa ggtagcagtc
     5881 cgccatagca ttccgttttc catagcagag cagagccggg gcgtgccgcc ccggatgtcg
     5941 ccaagaactt gcaggcgccg aatccaattc agcgcaaccg cacacagcta actggcaata
     6001 ctcaagccga gcggcgggcg ttgatcaggt cgcccagcag cccgagtccc aggagcagta
     6061 tc
//
LOCUS       DAOSYS010000214         6032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11058_length_6032_cov_5.010206, whole genome shotgun sequence.
ACCESSION   DAOSYS010000214 DAOSYS010000000
VERSION     DAOSYS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11058_length_6032_cov_5.010206"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="csb2"
                     /locus_tag="PLU99_12220"
     CDS             complement(<1..1253)
                     /gene="csb2"
                     /locus_tag="PLU99_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HOO17859.1"
                     /translation="MCGTPQFLCVSVTFLQPTFHGRRERGVPEWPPSPLRVFQALVAA
                     AAARWDQAERLAPMQALRWLESLSPPRIVAPVGISSSPYVISVPNNAMDLVASAWCRG
                     NTTGKDAQPATHRTMKTVRPTRLSDGDTVHYLWALEREDSVTSHPHIAWITGAARSIV
                     ALGWGIDVAIGDARLLSASEAAQLAGQRWHVRARGQALRVPDVGTLDALVRRHNAFVT
                     RLAGGCLTPVPPLDRFRVVRYAREAAVPPRPFAAFALRPVDPTSRSPWRAFRAERAAI
                     VAAMLRHAACRAAQADQGHWDPVPGGSNIYVAGHTRDDPSLRRGRTPPRFSYLALPSI
                     GARHADGMIRRVLIAEPFDGDGQHADWAAARLSGADLVDEERGPVATLERLDEHDPGE
                     RVLPRFTGRSREWISATPVVLPGYDG"
     gene            complement(1265..2338)
                     /gene="cas7u"
                     /locus_tag="PLU99_12225"
     CDS             complement(1265..2338)
                     /gene="cas7u"
                     /locus_tag="PLU99_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HOO17860.1"
                     /translation="MTDLLTKYDHWLADDGPAALVIRENLMPVEGRDGVLFPATFAAG
                     ENFPGGYNIDGPLDGENICLVDTVGSQANRIEPLFATGEYAKLVPQIVVAAGETSVNV
                     LLAGHRAGDALVRCSELQEELRQAFKAVQRGDATPLAKIAPTSLVFGVWDSRDTQAKL
                     PRLVASTIRAFCVRKLTRSAQYVPAASYVDDGLLDEPPDKNTKERYAERGFIHVPASA
                     THGGVIATGGIRRDATLLLAALRLLRSGDAESNTRALQRYVLGLALTAFTHPSAPVGY
                     LRQGCTLVRDPDKTGEFAEVYPDGRRVPADVTHAAALEYARAAAEAFGVGESRTVPFD
                     KERAKRDVQGDGSKKPRAKKNSK"
     gene            complement(2331..3104)
                     /gene="cas8c"
                     /locus_tag="PLU99_12230"
     CDS             complement(2331..3104)
                     /gene="cas8c"
                     /locus_tag="PLU99_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Cas8c"
                     /protein_id="HOO17861.1"
                     /translation="MSTHEFELAVHAANPGQFFACCGLLELSHRKWPGSEGWFEDGRF
                     HIACGGTLAELGAQICAAAPLQLDPDDDMTSPLFLPAPFNLRLDWWKDGLGGGSDFKT
                     WAGQQKVVRIAAAMHAALGAVAWSPDELLNSGAILYDIQDSNRPVEPFYFDCRRASQA
                     HSLDVGFSADAHNMNVPVYSAVEFMCLIGLQRFRPKPRDDRRFEYRTWTCPLAAPCAA
                     PAALGAIPLPGRSFAFPLLYRTKYLKGFLPAMKLENNYD"
     gene            complement(3101..5884)
                     /gene="cas3u"
                     /locus_tag="PLU99_12235"
     CDS             complement(3101..5884)
                     /gene="cas3u"
                     /locus_tag="PLU99_12235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HOO17862.1"
                     /translation="MNATDHFDGLFSAFCGVPSPLRWQRRLFAGFVQGRVPQICDLPT
                     GLGKTSVIPIWLIALAHSAQTNGASRKLPRRLVYIVNRRTVVDQATDHAKRLLALIYR
                     SGQLDHMPWASDEAIAELGLSKEAQVQPEHAPLVGALRKTLSSLCARSDVPPLAVSTL
                     RGELADNGEWKTDPARPAIIVGTVDMIGSKLLFSGYGDGRNARAQHAGLIGQDTLIVH
                     DEAHLSPAFSRLLWTVEREQRQGGDPRPISVLELTATSRDDAETTAVRQAVWSLRGTE
                     GDPVFSVAAEDGADSVVRRRLTARKKLTVEACDGGKGELIRAITAKALTYRDSKARVL
                     VYVRSPEDAKEIASALTNAKSGLGPDAEHRVALLTGTIRGHERDKLAATPLFREFKSR
                     DDRPPPEQTLYLICTSAGEVGVDFDADHLICDLTTLDSMAQRFGRVNRMGGKERCADV
                     TVFRASLDANITEKKKEAELLASAIAQTAHIMENVAANGRDVSPGALAEILCSSDAAP
                     AFSPHPMTLPATDILFDSWALTSITGVLPGRPEVGTYLHGTSQYDPPETYIAWRAEIN
                     DLARVGVTEDDLAEMLEVFPLRTAERLKDRVDRVQHELEGIAARHPDARVILIRDGEP
                     QWAALGDLAPTQKAEKERARRMLAYATVILPAEVGGLRSGMLDGQSEEPVLDVAEISG
                     SGQVARQRVRGNQEAEAALLGGDLLAAPVRWVVPLRGAEDPDEDVSAETIEYRVAKGE
                     DAEPGTPVLLAEHLGAVETRARSITRALRLPGDVAEAVGLAARWHDRGKARECWQRYA
                     CNKDVAQPLGKSRRYANPRVLGGYRHEFGSLHEASVDTEIAVHPERDLILHLISAHHG
                     WGRPHFRREPRGRADDPARTTDENQREIIEAARRFARVQRRFGRWGLAWLESLLRCAD
                     AIASDLPNQRGEV"
BASE COUNT         1083 a         1905 c         1959 g         1085 t
ORIGIN      
        1 ccgtcatagc cgggcaggac gaccggggtc gcactgatcc actcgcgcga acgtccggtg
       61 aagcgcggca acacgcgctc gccgggatcg tgctcatcca gcctttcaag cgtcgccact
      121 gggccgcgct cttcgtccac caggtcggcg cccgagagac gcgccgcagc ccaatcggcg
      181 tgttgtccgt cgccatcgaa cggctcggcg atgaggacgc ggcggatcat gccgtcggcg
      241 tgccgcgcgc cgatcgacgg cagcgccagg tacgaaaagc gcggcggcgt gcgtccgcgt
      301 ctgagtgatg gatcatcgcg cgtgtgtccg gccacataga tattggagcc gcccgggacg
      361 gggtcccaat ggccctggtc cgcctgcgcg gcgcggcagg cggcgtgacg cagcatggcg
      421 gcgacgatcg cggcacgttc ggcgcgaaaa gcgcgccatg ggctgcgtga agtggggtca
      481 accgggcgga gggcgaaagc ggcgaagggg cggggcggaa cggccgcctc gcgcgcgtag
      541 cgcaccacgc gaaaacgatc caatggcggg actggcgtga gacagccgcc ggccaagcga
      601 gttacaaagg cgttgtgccg ccgcacaagc gcgtccagcg tgccgacatc gggaacgcgc
      661 aacgcctggc cgcgcgctcg tacgtgccac cgctgtccgg ccaactgcgc cgcctccgaa
      721 gcgctcagca agcgcgcgtc cccgatggcc acgtcaattc cccagccgag cgcgacaatg
      781 ctccgcgcag cgcccgtgat ccaggcgatg tgggggtggc tagtgacgga gtcctcgcgc
      841 tcgagtgccc acaggtaatg tacagtgtcg ccgtcgctca gacgcgtcgg gcggacagtc
      901 ttcatcgtcc ggtgcgtcgc cggttgcgca tccttgcccg ttgtgtttcc gcgacaccag
      961 gcgctcgcta cgagatccat ggcgttattg ggaacggaaa tcacatatgg tgaactgcta
     1021 attccgactg gcgcgacgat ccgtggcggg gagagcgact ccagccatcg gagcgcctgc
     1081 atcggagcca ggcgctccgc ctggtcccag cgggcggcag ccgccgcgac cagggcctgg
     1141 aagacgcgta gcggcgacgg cggccattca ggcaccccgc gctcgcggcg cccatggaag
     1201 gtcggctgaa ggaaggtgac ggaaacgcag aggaactgcg gcgtgccgca catggatgtt
     1261 cctcctactt gctgtttttc ttagcgcggg gtttcttgct gccgtcgcct tggacgtcgc
     1321 gctttgcccg ttccttgtcg aaggggaccg tgcgactctc gccaaccccg aaggcctcgg
     1381 ccgcagcgcg ggcgtattcc agcgcggccg cgtgcgtgac atccgccggg acgcgacgcc
     1441 catcaggata aacttctgcg aattcgcctg tcttgtccgg gtcgcgaacc aatgtacagc
     1501 cctggcgcaa gtagccgaca ggcgccgacg gatgcgtgaa cgcggtcaaa gcaagcccca
     1561 gcacatagcg ttgcaaagct cgcgtattgc tttccgcatc cccacttcgc agcagacgca
     1621 atgcggccag caagagcgta gcgtcacgcc ggataccgcc ggtggcaatg acgccaccgt
     1681 gcgtggccga ggccggaacg tggatgaacc cgcgttctgc gtacctttcc ttggtgttct
     1741 tgtcgggggg ctcgtccagg agcccgtcat ccacgtagct ggccgctggc acgtactgag
     1801 ccgagcgcgt cagttttcgc acgcaaaacg cgcggatggt ggaggcgacc aggcggggca
     1861 gcttggcctg cgtatcgcgc gagtcccaca cgccgaacac cagtgacgtc ggggcgatct
     1921 tggcgagcgg cgtagcatcg ccccgttgta ccgccttgaa agcctgccgc agctcttcct
     1981 gaagctccga gcaacgaacg agagcgtcgc ccgcgcgatg gcccgccagc aatacgttaa
     2041 cgctagtttc tccggcagcg accactatct gcggcaccag cttcgcgtat tcgcctgtag
     2101 caaatagggg ttctatacgg ttagcttgtg aaccgacggt gtcaacgagg cagatgtttt
     2161 cgccatccag tggaccgtcg atgttgtagc caccggggaa attttcgcct gcggcgaacg
     2221 tggccgggaa gaggacgccg tcgcgaccct cgacgggcat cagattctca cgaatcacta
     2281 gtgcggcggg tccgtcgtcc gcgagccagt ggtcgtactt ggtaagaagg tcagtcatag
     2341 ttgttctcca atttcatggc aggcagaaag cccttgagat acttggtgcg atacaaaagg
     2401 ggaaatgcga acgacctccc agggagagga atagccccaa gcgcagcagg cgccgcgcac
     2461 ggtgccgcaa gcggacaagt ccacgtccgg tactcgaaac gtcggtcgtc acgcggttta
     2521 ggccgaaagc gctggagccc gatgaggcac atgaactcga cggccgagta aaccggcacg
     2581 ttcatattgt gagcgtcagc cgagaagccc acatcgaggc tgtgagcttg ggacgcccgc
     2641 cggcagtcga agtaaaacgg ctcgaccggc ctgttgctgt cttgaatgtc gtacaagatc
     2701 gcgccgctat tgagcaattc gtctggcgac caggctacgg cgccgagcgc ggcgtgcatg
     2761 gccgcggcaa tgcgaaccac cttctgctgg cccgcccagg tcttgaaatc gcttcctccg
     2821 cccagaccgt ccttccacca gtcgaggcgc aggttgaacg gagcgggcag aaaaagcggc
     2881 gaagtcatat cgtcatctgg gtcgagctgg agcggggctg cggcacagat ttgggcgcct
     2941 agttccgcaa gcgttccgcc gcaagcgatg tggaatcgcc catcttcaaa ccaaccctca
     3001 ctgcccggcc acttccggtg gcttagttcc aacagtccgc agcaggcaaa gaactgtccg
     3061 gggttggccg cgtgtacggc aagttcgaat tcatgcgtgc tcatacttca ccccgctggt
     3121 tgggcaggtc agatgcaatg gcgtccgcgc agcgcaggag agattccagc cacgccaagc
     3181 cccaacgccc gaagcgccgc tgaacgcgcg cgaaacggcg agcggcttcg attatctcgc
     3241 gctggttttc gtccgtcgtg cgcgccgggt cgtccgcgcg cccgcgcggc tctcggcgga
     3301 agtgcgggcg accccaacca tggtgcgcgg atatgaggtg cagaattaag tcgcgctctg
     3361 ggtgcaccgc gatctcggtg tcgactgaag cctcatggag cgagccgaac tcgtgccggt
     3421 agccgcccag cacgcgcgga ttagcgtagc gtctggattt gcctaagggc tgggcgacgt
     3481 ctttattgca ggcgtaccgc tgccaacact cgcgcgcttt gcctcggtca tgccagcgcg
     3541 ccgcgagtcc caccgcttcc gcaacgtcgc ctggcagacg cagagctcgc gttatgctgc
     3601 gggcgcgcgt ctcgactgcg cccaaatgtt cagcgagcaa gactggcgta cccggttcgg
     3661 cgtcttcgcc tttggctacg cggtattcga tcgtctccgc agaaacgtcc tcgtcagggt
     3721 cttctgcgcc ccgcagaggg actacccacc tgaccggcgc tgccagcaag tcgccgccca
     3781 agagggcggc ttcagcctct tggttcccgc gaacgcgctg gcgcgccacc tgacccgacc
     3841 cggaaatttc agccacgtcc aagaccggct cctcggactg cccgtcaagc atcccgctgc
     3901 gcagccctcc tacttcggca ggaagaatca ccgtggcata cgccagcatg cggcgcgccc
     3961 tctccttttc tgctttttgc gtgggcgcca gatcgccaag cgcggcccat tgcggctcgc
     4021 catccctgat caggatgacg cgcgcgtccg ggtgtcgggc ggcaatgccc tccagttcat
     4081 gctggacgcg gtcgacgcga tccttgagac gttcagccgt gcgaagtggg aacacctcga
     4141 gcatttcggc caaatcgtct tcggtaacgc ccacccgcgc cagatcatta atttccgcac
     4201 gccaggcgat ataggtctcg ggcggatcgt actggctggt gccgtgcaga tatgttccca
     4261 cttccggccg cccgggaagc acgccggtga tggacgtcag cgcccacgag tcgaatagaa
     4321 tatctgtggc cggaagggtc atagggtggg gcgagaatgc tggagccgca tcagaggagc
     4381 agagtatctc ggccagtgcg ccgggggaca cgtcgcggcc attagccgcg acattttcca
     4441 tgatgtgggc ggtctgggcg atggccgagg cgagcagctc cgcctccttc ttcttctctg
     4501 taatgttggc gtccaggctg gctcggaaga cggtgacatc tgcgcaacgc tccttcccac
     4561 ccattcggtt cacccgccca aaacgttgcg ccatgctatc aagagtcgtt aggtcgcaga
     4621 tgagatgatc cgcgtcgaag tcaacgccca cttcgcccgc agaggtgcag attagataca
     4681 gcgtctgctc cggcggcggt cgatcgtccc gcgatttgaa ttcccgaaaa agcggcgtgg
     4741 cggcgagttt gtcgcgctca tgcccgcgaa tggtgccggt aagtaacgct acccgatgct
     4801 cggcgtcagg tcccaaaccg cttttggcgt tcgtgagagc gctggcgatc tctttcgcgt
     4861 cttcaggcga ccggacgtaa accagtacgc gtgctttcga atcgcgataa gtgagcgctt
     4921 tggcagtaat cgcgcgaatc agttcgccct tgccgccatc gcacgcctca accgtcaatt
     4981 tcttgcgggc agtgaggcgg cgcctgacga cagagtcggc gccgtcctca gcagccacgc
     5041 tgaaaacggg atcgccctcg gtgccgcgga gcgaccatac cgcctgccgt acggctgtgg
     5101 tctcagcgtc gtcgcgggaa gtggcggtca attcgagaac agatattgga cggggatcac
     5161 ccccttgcct ctgctcgcgc tctaccgtcc acagcaagcg tgagaaagcg ggcgacagat
     5221 gcgcttcgtc gtgaacgatc agcgtatcct gcccgatcaa gccggcgtgt tgggcgcgtg
     5281 cgttgcgtcc atcgccgtag ccggagaaca ggagcttgct gccgatcatg tcgactgtgc
     5341 cgacgataat tgctggacgg gcagggtcgg ttttccattc gccgttgtcg gcgagttcgc
     5401 cgcggagggt gctgaccgcg aggggaggta cgtcgctgcg ggcgcacaag gatgaaagcg
     5461 ttttgcgcag tgcgcctacc agtggcgcgt gctcgggctg gacctgcgct tcctttgaca
     5521 ggccaagctc tgcgattgcc tcgtcactgg cccacggcat atggtcgagt tgccccgaac
     5581 ggtagatcaa agcgagcaga cgcttggcgt ggtcagttgc ttgatcgaca acggttcgcc
     5641 ggttgacgat gtagacgagg cgccgtggta gcttgcggga ggcaccgttg gtctgggcag
     5701 agtgcgcaag agcaatcagc cagatcggga tgaccgaggt tttgccgagg ccggtgggca
     5761 ggtcgcatat ctgtggcact cggccttgta cgaaaccggc aaacagcctg cgttgccagc
     5821 gaagcggcga cggcacgccg caaaatgccg agaaaagccc gtcgaaatgg tcggttgcgt
     5881 tcatgtgctc gccctcaagg aaacaaaagc ctaacacggg tttgcggctt attcaagttc
     5941 cgcggggctc ggctggccgg ctcctgcccg gttcctggcc ggttacaact gctcccgcac
     6001 gctgtacgat gccggccagg gagtttggct ga
//
LOCUS       DAOSYS010000215         6019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11092_length_6019_cov_4.790744, whole genome shotgun sequence.
ACCESSION   DAOSYS010000215 DAOSYS010000000
VERSION     DAOSYS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11092_length_6019_cov_4.790744"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLU99_12240"
     CDS             <1..186
                     /locus_tag="PLU99_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17863.1"
                     /translation="GIELLIDYDGQSGSVRWAEFRNLTPAGGTIRLQPGAIQLDPAVL
                     DPKHWITDATEITEIYE"
     gene            322..654
                     /locus_tag="PLU99_12245"
     CDS             322..654
                     /locus_tag="PLU99_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOO17864.1"
                     /translation="MKTVLVLGRYGMGEGDPALGVKILGTFLRKSPAIRELQAIVLFN
                     AGVKLAIEGSPVLVELLQLHENGVEIHPCGTCLEFFGLKDKVRVGGVSNMDTIISVLN
                     AAEKVITL"
     gene            873..1607
                     /locus_tag="PLU99_12250"
     CDS             873..1607
                     /locus_tag="PLU99_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009817338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOO17865.1"
                     /translation="MKKYLAEFIGTFTLVFFGCGTAVVAGERVGFLGIGLAFGLALIA
                     MAYGIGPISGCHINPAVSLGVFTAGRMNRRDLIGYIVAQVIGAFVAAIVLAAVASGRA
                     GYSIGENGLGQNGWGPGMFNEYNFGAALIFELVASFLFVVVVLGSTGAGAPATLAGLA
                     IGLALAAIHIFGIPITGTSVNPARSLGPALVVGGKALAQVWLFLVIPSLSGIVAGWLF
                     RTGILESRAPAPSATMGTARKATTDE"
     gene            complement(1664..2275)
                     /locus_tag="PLU99_12255"
     CDS             complement(1664..2275)
                     /locus_tag="PLU99_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17866.1"
                     /translation="MRGILLVIGAVAISGCAAPQADRLSSPRSPARFSEPDWCRAQDG
                     LAAGEWNADAPTVKLELVFFKLDSQEARENAERLGLASGAAVLTDEQFTDLLESARSG
                     GAVTAWPGIEAYAGGAASLELGRTDGERILRGTAWHMRTCNVTADSADVQFAVQIIAD
                     ENNWFVEDFQQLQLGEAVAHLVAAGDQERPQQGLAVRLASIYP"
     gene            2438..2659
                     /locus_tag="PLU99_12260"
     CDS             2438..2659
                     /locus_tag="PLU99_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOO17867.1"
                     /translation="MPIYEYTCSGCGRLFEQLVQSREQEAELACPHCGSRKLERQLSV
                     PAAPRVAAGSTPPPPCGGCNQAGGSCPYR"
     gene            2644..3207
                     /locus_tag="PLU99_12265"
     CDS             2644..3207
                     /locus_tag="PLU99_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17868.1"
                     /translation="MPVQMSHEESGQTTRAAGTAKTVQAGLLSWILPGAGHWMLGHRG
                     LAVVFFVAISLPFWVGLAIGGIKTSVNPWSNPWLFLAEMGTGGYTSLSLIANNMIGGL
                     DPRALADPRYLNKVSPELREKYVSYMSFYPESEVAQIYLATAGLLNILVILDAIARAQ
                     TGGLPTFPPEPEREPPPSEAAVPGAGS"
     gene            3204..3521
                     /locus_tag="PLU99_12270"
     CDS             3204..3521
                     /locus_tag="PLU99_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17869.1"
                     /translation="MIVPPAGFLAHLDWSLLSRATGGVGSALLGWVPFLNPVKLPSGA
                     RLWMFLPLALCIAVVYRATRARSLEKLPRGTLVTFVNITVGMIAIAAAFYLVNKAALF
                     LGP"
     gene            3606..3695
                     /locus_tag="PLU99_12275"
     tRNA            3606..3695
                     /locus_tag="PLU99_12275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3640..3642,aa:Ser,seq:gct)
     gene            3928..4386
                     /locus_tag="PLU99_12280"
     CDS             3928..4386
                     /locus_tag="PLU99_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17870.1"
                     /translation="MEFGLLAVVLAVATVGCGSSSGNVVAFGTAPVPPAMQHPRLEGF
                     PIPPGFALLDKKTVIGKNGDVRTAHCEFTGLLSIAELDRFYRKTMPEAGFTLRNERLT
                     HGEYCLEFTGPRESSTVSFKVDRKKTLLVVDLYPTSGAEVAPQPKPKTQP"
     gene            4422..5366
                     /locus_tag="PLU99_12285"
     CDS             4422..5366
                     /locus_tag="PLU99_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17871.1"
                     /translation="MDADKRHYLKQNELAEALTKLRNLSSHREVLYWVAAVAVVLLAF
                     VGYRGWGSLQASLAAKSWHELSQVSQKVQIKTVGALDELRGIISNASNPTLAASARIQ
                     LAAALRESATQTGTIDQAALQESVEVLKPLLNQRDLSPTLAGAAALSLASSYETLGQF
                     DEAEQTYNIILSDQQRFAGTGYANVAAERLETLPELRKPVEFAAGLPPPPGIPTITVQ
                     QPGSTSPEPLELTPVGPPSPPLPPAEAPESGSPDAGAPAEEPAPAGNAVPGEEPAPAE
                     ESATSGEAPGQQAGNAVGGEPGAQTPVPATQPVGNQGP"
     gene            5363..>6019
                     /locus_tag="PLU99_12290"
     CDS             5363..>6019
                     /locus_tag="PLU99_12290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOO17872.1"
                     /translation="MTTYEDEILDRPIPVDDDEAQRVRLRIRRKLPGRRLDKYLHGRF
                     PHLSRTTIQRLIKQGAVTVNNQPTKPSYEMAGGDIVELVIPPPEPREVVPENIPLDVV
                     YEDDFILALNKPAGIICHPARGDQTGTIANALVYYANELSHGADPFRPGIVHRLDKNT
                     TGIMLTAKTDEAHWRLSLQFERRTIQKTYLAVAHGEFDLDGDTINAPIGVHPVVREKF
                     A"
BASE COUNT         1071 a         1826 c         1977 g         1145 t
ORIGIN      
        1 ggcatcgaac tgctgatcga ctacgacggg caaagcggtt cggtccgctg ggcggaattt
       61 cggaatctca caccggccgg tggcacgatt cgccttcagc ccggggccat acagttggat
      121 ccggcggtgc tggacccgaa gcattggatt accgacgcaa ccgagattac cgagatttac
      181 gaataaggtg gagaaacccc ccaaaactcg gctcgcccag gcaaacctcc gccggggtga
      241 gcttttttcc ggagaggagg ggtaacttcc ggcccgcgga gctaaactct tcgtgagctt
      301 ccgcaaagaa caggagcgac gatgaagact gtgctcgtgc ttggccggta cggaatgggt
      361 gaaggcgacc ccgcgctggg agtgaaaatc ctgggtacgt ttttgcgcaa gtcgcctgcg
      421 atacgggagt tgcaggccat cgtgcttttc aacgcgggtg tcaagctggc catcgagggc
      481 tcgccggtgc tggtcgagct gctgcaactg cacgagaacg gggtcgaaat ccatccctgc
      541 ggcacctgtc tggagttctt cgggctcaag gacaaggtgc gtgtcggcgg ggtgtcgaac
      601 atggacacca tcatcagcgt gctcaacgcg gcggaaaagg tcatcactct ttagtcaacg
      661 ggcccggcgg cctgcacgtt tgatccgcac gttggctgcg atttcgcagc aattggaccg
      721 ggaaagtcag gccttcagcc tgcgcgcggg tcaaccagcg ccgcctttgc atttgattat
      781 taaagcgaca cctgtgtagt ttccggtgtt cactctgttt cccagatata attaaccgcg
      841 gggctggcgg ctgaagtgcc cggagtcttg tcatgaaaaa gtacctggcg gaattcatcg
      901 gcacgtttac cctggtgttc ttcggctgcg gaacggctgt cgtggccggc gagcgcgtgg
      961 gtttcctcgg catcgggctg gcattcggcc tggcgctgat cgcaatggcg tacggcatcg
     1021 gccccatttc gggttgccac attaaccccg ccgtcagcct gggcgtgttc acggccggac
     1081 gaatgaaccg ccgcgatttg atcggctaca tcgttgcgca ggtgataggg gcgttcgtgg
     1141 cggcaatcgt cctggcggca gtcgcgtcag gccgggccgg atacagcatc ggcgaaaatg
     1201 gcctggggca gaatggctgg ggccccggga tgttcaacga atacaacttc ggcgcggcgc
     1261 ttatttttga gctggtggcc tcgttcctat ttgtcgtggt tgtgctcggt tcgacggggg
     1321 cgggtgcgcc ggcgacgctg gcagggctgg caatcggtct ggcactggcg gccattcaca
     1381 ttttcggaat tccgatcacc ggcacgtcgg tgaacccggc ccgcagcctg gggccggcat
     1441 tggtcgtcgg cgggaaggcc ctggctcagg tatggctgtt cctggtgatt ccgagcctca
     1501 gcggcatcgt cgccggctgg ctgttccgaa ccggcattct ggaaagccgg gcgcctgctc
     1561 ccagcgcgac catgggaacg gcgcgcaagg ccaccacgga cgagtaagcc gggcttaaga
     1621 acagacgcgg ccgattcggc cggtatcacc gattcggccg gtatcaagga tagattgagg
     1681 ccagccgcac ggccaagccc tgctgcggcc gctcttgatc gcctgccgcg accaggtggg
     1741 cgaccgcttc gccgagctgt agctgctgga agtcttcaac gaaccagttg ttctcgtcgg
     1801 cgatgatctg aacggcgaac tgcacgtcgg cactgtcggc ggtcacattg caggtgcgca
     1861 tgtgccaagc cgttccgcgc agaatccgct cgccgtcagt cctgccgagc tcgagcgatg
     1921 ccgctccgcc tgcgtaagct tcgatccccg gccaggcggt cacggcgcct ccagagcggg
     1981 cgctttcaag caggtccgtg aattgttcgt cggtcagaac cgccgcaccc gacgccaggc
     2041 ccaggcgctc ggcgttctca cgcgcctctt ggctgtcgag tttgaaaaac accaattcca
     2101 gcttcaccgt cggcgcgtcc gcgttccatt cccccgctgc caacccgtcc tgggcgcggc
     2161 accagtcggg ctccgaaaac ctcgccggac tccgcggcga cgaaagtcga tcagcctgag
     2221 gtgctgcaca accggaaatc gcgacggccc cgataacgag cagaattccg cgcacgactc
     2281 gacctccttc tttcaaacga ggcaggctca ttgtaacgcc cgtaatcgcg tgccgcgagt
     2341 tgtagcgtgg ccgggtgccg gcccggctgg cgccgcggct tgccccccga cgcggcccct
     2401 cctatacttt tggcttcaaa cgcgtttcgg agtttgcatg ccgatatacg agtacacgtg
     2461 ttccggttgt ggccggttgt tcgagcagct cgtgcagtcg cgtgagcaag aggccgaatt
     2521 ggcctgcccg cactgcggta gccgaaagct cgaacggcag cttagcgtcc cggcagcccc
     2581 acgcgttgcg gccgggtcga cgccgcctcc tccttgcggc gggtgcaacc aggcgggtgg
     2641 gtcgtgcccg tacagatgag tcacgaagag agcggacaaa cgacgcgggc agccggtacg
     2701 gccaaaacgg ttcaggccgg cttgctgagc tggattttgc ctggtgcggg gcactggatg
     2761 ctgggccacc gaggcctggc ggtggtcttc tttgtggcca tcagtttgcc gttctgggtc
     2821 ggtttggcga tcgggggcat caagacttcg gtcaatccgt ggagtaaccc gtggctgttc
     2881 ctggccgaga tgggcaccgg gggctacacg agcctgtcgc tgattgcgaa taacatgatc
     2941 ggcggcctgg acccgcgggc gctggcggat ccgagatact tgaacaaagt cagcccggag
     3001 ctgcgcgaga agtacgtgtc gtacatgagt ttctatcctg agtcggaagt agctcagatt
     3061 tatctcgcga ccgccgggtt gttgaacatc ctcgtgattc tggacgcgat tgcgcgggcc
     3121 caaacgggtg ggttgccgac gtttccgcct gagcctgagc gagagccgcc gccgtcagag
     3181 gccgccgtcc cgggagccgg gtcatgatcg tcccgccggc tggttttctg gcgcacctgg
     3241 actggagtct gctatcgcgg gcgacgggcg gtgtggggtc tgcgttgctg ggctgggtgc
     3301 cgtttctgaa cccggtcaag ctgccgtccg gagcgcgact gtggatgttt ctgccgctgg
     3361 cgttgtgcat cgcggtggtg taccgggcca cgcgagcgcg ttcgctcgag aagctgccgc
     3421 ggggcacact ggtgacgttc gtgaacatca cggtcgggat gatcgcgatc gcggcggcgt
     3481 tctacctggt gaacaaggcg gcccttttcc tggggccgta agcttgttgg cgccgccggg
     3541 cttgcctgag aggtgcggta aggggtagta tcttggggat ttttcagcgt attgctgaat
     3601 cgcctggaga ggtgaccgag tggctgaagg taacggtttg ctaaaccgtc gtactccgta
     3661 aaggggtacc gcgggttcga atcccgccct ctccgttaca tttgaccgaa tgatcgaagg
     3721 cgtttttggg ttggcgaacc ttcgctgatc gcgaaagcgc cgcacagctc ctgcgagtgt
     3781 tagcaaccgg tcgtatgcgt acgtgctatt gctcacacgg ggtttgacga aacggctgat
     3841 aagtggcaaa attctctttt tgggaagcgt ggcggagact cgtcaatgag tttcagggca
     3901 agcttgagaa cgcgcgatgc gcggctggtg gaattcggcc tgttagctgt cgttctcgcg
     3961 gtggcaaccg tgggctgcgg ctccagctcc ggaaacgtcg tggcgtttgg cacggcgccg
     4021 gtgccgccgg ccatgcagca cccgcggctg gaggggtttc cgatcccgcc ggggttcgcc
     4081 cttttggaca agaaaaccgt tatagggaag aacggcgatg tccgcaccgc gcactgtgaa
     4141 tttactgggt tgctgtcgat cgcggagctc gaccgcttct atcggaagac catgccggaa
     4201 gcgggtttta cgttgcgtaa cgagcgcctg acccacggcg aatattgcct cgagttcacc
     4261 ggccctagag aatccagcac ggtcagcttt aaagtcgatc gcaagaagac gctgctcgtg
     4321 gtcgacttgt acccgacgtc cggcgccgaa gtcgcgccgc aaccgaagcc gaaaacgcag
     4381 ccttgaccta cgtgcggctg caaaacaagt agaggagcac catggacgcc gataaacggc
     4441 attatcttaa gcaaaacgaa ctggccgagg cgctcaccaa gctgcgcaat ttgagcagcc
     4501 accgcgaggt gttgtattgg gtggcggccg ttgcggtcgt gctgctggcc tttgtgggct
     4561 atcgcggttg gggctcgctg caggccagtc ttgcggccaa atcctggcac gagctgtcgc
     4621 aagtcagcca gaaggtccag atcaagacgg tgggcgcgct ggacgagctg cgcggcatta
     4681 tctccaacgc ttcgaacccg acgctggcgg cgtccgcgcg aattcagttg gccgcggcct
     4741 tgcgcgaaag tgcaacccag accggcacga tcgatcaggc ggcgctgcag gagtcggtcg
     4801 aggtcctcaa accgctgctg aaccagcgcg acctgtcgcc gacgctcgcc ggagcggccg
     4861 cactgtcgct ggccagctcg tacgagacgc tggggcaatt cgacgaggcg gagcagacgt
     4921 ataacatcat cctgtcggat cagcagcgtt tcgccggcac gggttatgcg aacgtagccg
     4981 cagagcgttt ggagacgctg cccgagttgc gcaagccggt ggaattcgcg gcaggcttgc
     5041 cgccgccgcc gggaataccg acgatcacgg tgcagcagcc gggttctacg tcgccggaac
     5101 cgctcgagtt gacgccggtg ggtcctccgt cgccgccgct gccgccagcc gaggcgcccg
     5161 agtcgggtag tccggatgca ggcgcgcccg ctgaagagcc cgcgcctgct ggtaacgccg
     5221 tgcccggtga agagcctgcg cccgctgaag agtcggcaac gagcggcgaa gcgcctgggc
     5281 agcaagcagg gaacgctgtc ggcggtgagc ccggcgctca gacccccgtg cctgcgacgc
     5341 agcccgttgg caatcagggt ccgtgacgac gtacgaagac gagattctcg atcgccccat
     5401 cccagtagac gatgatgaag cgcagcgcgt gcgcctgcgc attcggcgca agctgcccgg
     5461 ccgccgcctc gataaatacc tgcacgggcg cttcccgcac ctgtcgcgca ccacgattca
     5521 gcggctgatc aagcaaggtg cggtcacggt caacaaccag ccgaccaagc ccagctacga
     5581 gatggccggg ggcgacatcg tcgaactggt aattccgccg cccgagccgc gcgaagtcgt
     5641 gcccgagaac atcccgctcg acgtggtcta tgaggacgac ttcatactcg ctttaaacaa
     5701 gccggccggg atcatctgcc atccggcccg cggcgatcag accggcacca tcgccaacgc
     5761 cctggtgtat tacgcgaacg agctcagcca cggcgccgat ccgtttcgtc ccggcatcgt
     5821 ccaccgcctc gacaagaaca cgaccggcat catgctgacc gccaaaaccg acgaggccca
     5881 ttggcgcctg tcgctgcaat tcgagcggcg cacgattcag aagacctatc tcgcggtcgc
     5941 gcacggcgag ttcgacctgg acggcgacac tatcaacgcc ccgatcggcg ttcatcccgt
     6001 agtacgcgag aagttcgcc
//
LOCUS       DAOSYS010000216         6001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11125_length_6001_cov_5.045409, whole genome shotgun sequence.
ACCESSION   DAOSYS010000216 DAOSYS010000000
VERSION     DAOSYS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11125_length_6001_cov_5.045409"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLU99_12295"
     CDS             <1..885
                     /locus_tag="PLU99_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17873.1"
                     /translation="DVRERLERDLKEANERAQGAAQHTQELEWQVVTLQTRVEALEQL
                     RQEVALLEEELATANAQLQAANEEQARSEKERARLRNELKTANKQLEELWALRKERDG
                     LRSDVASLTSRIEELDRAQRELTDERGALAARLKETAVALEEARGERHQHQLLLRAAE
                     DRARELTRVQEELADKLETLRADKKALQVQVGQLERENARLVEQRQFYETELSSMRNA
                     SRSAELALSSVKKAFAEVRVALTETKSRARRRMIDTWPRIGMPLRGIGDAAEIDQADE
                     ATGQTIAHDSDAVSAGKA"
     gene            1114..2322
                     /locus_tag="PLU99_12300"
     CDS             1114..2322
                     /locus_tag="PLU99_12300"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOO17874.1"
                     /translation="MNPQTPHTPVAAAASDAFRLRLDLCDEAAAGPLAGTTFAVKDMF
                     DLAGLVTGGGTPDWLATHAPARDTAPCVVACLRAGARLVGVTIADEMAFSITGENAHY
                     GTPLNPAAPDRVPGGSSSGSASVTASGLADFALGTDTAGSIRVPASYCGIFGIRPTHG
                     RISTQGVMPLSPSFDTVGWFARDGGLLERVGKVLLGENDAGGQPIRRLLLLDDAFELL
                     DPEVRAPLERAAEELAATIAPISRTTLSSEGYADWLACFNALRPPEIWAIYGDWITRT
                     QPRFGPEIAARFEAVKRTAHADTTAARRFRAAIQEKAARMLEDGGAFLLPTVPTIAPL
                     KGTPAAATQALRENTFRLTCISPLLGTPEVSLPLATADGCPIGLSLVGPTGGDASLLR
                     AAAQLTPRRG"
     gene            complement(2345..5944)
                     /locus_tag="PLU99_12305"
     CDS             complement(2345..5944)
                     /locus_tag="PLU99_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17875.1"
                     /translation="MTWLSIAGLVLTAGLYLYFTNSGRLRRQLLAAFDEWPVEAVSLD
                     GISFTPWHGLEVRGLYLALRGPGSLADDAPCVRAVLRIERTRIRCRLGALLKGEFVPR
                     WAALDRADVAVVLPETGGATDPLGDTAGLEIEDLANSSAGRGILQMLRRGRPIPELRL
                     GDTNIRVSQSDPRGAQLVRHWRVVGEGAWSDGVYALELRRTGDKGEPLFTLRWDPQSE
                     TCESACDWLELDTLAVFFPRIAEAWSDLGLRGRGRVRRIALRTDLGAESSSGAESPGG
                     LPLPLTAVDLQFAGLRCSIPVEVPEAGRPPVPAWSRFLQVSNAELTLNCTPESNGRGL
                     VKLTGTGLVNRAPAAFKLTADLAALLEPAAAQDHVVSAELNVTGMHFPTRPGDAAFID
                     SPRLPLSVRNVLNDFRPSGLFSLLVQARRVWDAETGGGRLHVEGWLDAQGVRCRYHTF
                     PYEFTEARGRTTFSPRGLDLDLTARHGPAHATATGHVNRAAHATGFDLTISGTNVPLD
                     GDLAAALPSKYRRLWDQTDPTGSCDVTVRLQREDGPPELPENPPTETFISADLGDGSS
                     ILLEPGKRLEQATGHFEIAEGFIEIQDLRGYLEGSPAMIAGTVGVDSGEADVRIAVSD
                     VPVKRSISVEERADDTPAQINFDGQADVAGRIHTAGEPGTRSDAYVVHLRRGMLTGFD
                     PELQWGDCHGRLIISDRESVIGSFEARQGAARLTAAGTLPGSKGGPVTLDVNIAQAEM
                     ETLARQLVPPRWRAQVEALGLAGLGDVQLQLRPESEPEGSARQIAEIQLSAERMEPSA
                     LPLDMSDIQAQARLTSDGVEIVEAQARCGEAGQLEVSGELSDSESGWTARFSAAARDV
                     DLSPSVTEALPAELARIVRRLEAQGRLQLEITAGARLAERDGVAWNFGGQLVLSDGAL
                     QLGVPLDDIEGGLSGTCRIDPAGGVGLDGALRIESGTLAGRRLAGLAGEIHYRAGERW
                     IRLDELRGQICGGDAVGFVHVDPVTSEYELSVTLQNVLLDEFLPKQPAQDPAALAGQR
                     RSARAGAMRRGVLDGYVFLRGHADDPASRQGGGELRVTGASILQTPVAAEVVEANPHA
                     EAVSDALQGAELRFTWDGAELHFTRLELLSPDLHLAGEGIWNMDSDQIAMTLVGAHPR
                     NWPRMAALGDLLETTERQLVQYHVTGTLAAPQVRAEPLYRLTEALRALILSGD"
BASE COUNT         1066 a         2173 c         1806 g          956 t
ORIGIN      
        1 gacgtgcgcg aacgcctgga acgcgacctc aaggaagcca acgagcgcgc ccaaggtgcc
       61 gctcagcata cccaggagct ggagtggcag gtcgttaccc tccaaacgcg ggtcgaagcg
      121 ctcgagcagt tgcgccagga ggtcgcgctg ctggaagagg agctcgccac tgccaacgct
      181 cagctacagg cagccaacga agaacaagcc cgcagcgaga aagaacgcgc ccgcctgcgg
      241 aacgagctaa aaacggccaa caagcaactc gaggagcttt gggccctgcg gaaggagcgc
      301 gacgggctgc gcagcgacgt agcatcgctg acctcgcgaa ttgaggagtt ggaccgcgcg
      361 cagcgcgagt tgaccgacga acgcggcgcc ctggcggctc gcctgaagga aaccgcggtc
      421 gcgctcgagg aagcccgtgg cgagcgccac cagcaccaac tcctgctgcg cgccgccgaa
      481 gaccgcgccc gcgaactcac ccgcgttcag gaggaactgg ccgacaagct cgagaccctg
      541 cgagccgaca agaaggccct gcaggtccag gtgggccagt tggaacgcga aaacgcgcgc
      601 ctggtcgaac agcgacaatt ctacgaaacg gaactctcct cgatgcgcaa cgccagccgc
      661 agtgccgagt tggccctgtc cagcgtcaag aaggcgttcg ccgaagtgcg cgtcgctttg
      721 accgagacca agtctcgcgc ccggcgccgc atgatcgaca cttggccgcg gattggcatg
      781 ccgctgcggg gtattggcga cgcggccgaa atcgaccaag ccgacgaggc gaccggccag
      841 accatcgcac acgattccga cgccgtgagc gctgggaaag cctgaacctg cttacgactc
      901 aattcctgcc ccggagggcg ccgtccgcaa gaccggcgcc cttttttgct tttctgcgcc
      961 aacttggctg accggccgtc ctgggcagcg cggctctgcc gacgaggcgc ggcgaccggc
     1021 accgcccctg agacagccaa accccctgct tgacgaacgc cgctcgccgc gagcctaata
     1081 ccgctaatct ggacacccga ctgctgatga gccgtgaacc cgcagacccc acacacaccc
     1141 gtcgccgccg cagcatctga cgcattccgg ctgcgcctcg atctctgcga cgaggccgcc
     1201 gccggtcccc tggccggcac gacgttcgcc gtcaaagaca tgttcgacct ggccggattg
     1261 gtcaccggcg gcggtactcc agattggctt gcgacccacg ccccggcccg cgatacggct
     1321 ccttgcgtcg tcgcctgctt gagggccggc gcgcggctgg ttggcgtcac gatcgccgac
     1381 gagatggctt tcagcatcac cggtgagaac gcccattacg gaacgccgct gaatcccgca
     1441 gcacctgacc gcgtgcccgg cggctcatcc agcggctcgg catcagtgac cgccagcggc
     1501 ctggcggact tcgcactggg caccgacacg gccggctcga ttcgcgttcc ggccagctat
     1561 tgcggcatat tcggaatacg tccaacacat gggcgaattt ccacgcaggg cgtaatgcct
     1621 ctctcgccga gcttcgacac agtcggctgg ttcgcccgcg acggcgggct gcttgaacgc
     1681 gtcggtaaag tgctcctcgg cgaaaacgac gccggcgggc agccaatccg ccggctgctg
     1741 ctgctcgacg acgccttcga gcttctcgat cccgaggtgc gggcacctct tgaacgcgcc
     1801 gccgaggaac tggccgcgac gatcgccccg atcagccgca ccacgctcag ctccgagggt
     1861 tatgccgact ggctggcatg tttcaacgca ctgcggccac ccgagatatg ggcgatctat
     1921 ggcgattgga ttacgcgtac ccagccgcgc ttcggcccag aaatcgccgc ccggttcgag
     1981 gcggtcaagc gaaccgcgca cgccgacacg accgcggcgc ggcgctttcg cgccgccatc
     2041 caggaaaaag cggcgcgaat gcttgaagac gggggcgctt tccttctgcc cactgtccca
     2101 acgattgcgc cccttaaggg tacgcccgcg gccgcaacgc aggcgctccg cgaaaacacg
     2161 tttcgtttga cgtgcatctc accgctattg ggaacgccag aggtctcctt gccgctggcg
     2221 accgcagacg gttgcccgat cggcctttcg ctagtcggcc caacaggcgg cgatgccagt
     2281 cttctgcgcg cggcggcaca actaactcca cgccgcggct aatggcgccc accgtcagtg
     2341 gctttcagtc tccactgagg attagcgcgc gcagcgcttc cgtcagccgg tacaacggtt
     2401 ccgccctgac ctgcggggcc gccagtgtgc ccgtcacgtg gtattgcaca agctggcgct
     2461 ccgtcgtttc gagcaaatcc cccaaagccg ccatgcgcgg ccagttacgc gggtgcgctc
     2521 caaccagcgt catggctatt tggtcggagt ccatgttcca aatcccttcg ccggccaggt
     2581 gcaggtcggg actcagcaac tccagtcgcg tgaaatgcaa ctcggcacca tcccacgtga
     2641 agcgcagctc ggccccctgc agtgcatcac tcacagcttc cgcgtgcgga ttcgcctcaa
     2701 ctacttcggc cgccacgggt gtctgcaaga tcgacgcccc cgtcacgcgc agctcgccac
     2761 cgccctgccg gctcgcggga tcatcagcat ggccgcgtag aaaaacatat ccatcaagaa
     2821 cgcccctgcg catggcgccg gctcgcgcag agcgccgctg cccagcgagc gccgccgggt
     2881 cttgggccgg ttgtttgggc aggaactcat cgagcagcac attttgcaat gtgaccgaaa
     2941 gctcgtattc tgacgtcacc gggtcaacat gcacgaatcc caccgcatcc ccgccgcaaa
     3001 tctgcccgcg cagctcatcc agcctgatcc agcgctcgcc cgcacgatag tgaatctcgc
     3061 ccgccagccc ggccagtcga cggcccgcca gcgtgccgct ttcgatacgc aacgccccgt
     3121 ccaggccgac cccgcccgcg ggatcgatgc gacacgttcc gcttagaccg ccctcgatat
     3181 catccaacgg cacaccaagc tgcaacgcgc cgtcggaaag cacaagctgt ccaccgaagt
     3241 tccatgcgac gccatcccgc tcggccaaac gcgccccggc ggtaatctcc agttgcagcc
     3301 ggccttgcgc ctccagccgc cgcacgatgc gcgccagctc ggccggaagc gcttcggtta
     3361 cgctgggcga gagatcgaca tcgcgcgctg ccgcgctgaa tcgggctgtc cagccgcttt
     3421 cgctgtccga cagctcgccc gatacctcca gttggccggc ctctccgcag cgcgcttgag
     3481 cctccacaat ctcaacgccg tctgacgtaa ggcgcgcctg cgcttggatg tcagacatgt
     3541 cgagcggcag cgcactcggc tccatgcgct cagccgacaa ctgaatttcc gcgatctgcc
     3601 gtgcgctgcc ttccggttcg ctttcgggcc gcagttggag ctgcacatcc cccaggccgg
     3661 ccagccccaa ggcctcaacc tgtgcccgcc agcgcggcgg aaccaattgt cgcgccagcg
     3721 tctccatttc ggcctgggcg atgttcacgt ccagcgtgac tggtccgcct ttgctccccg
     3781 gcagcgttcc ggcggccgtc agccgcgccg cgccctgccg cgcctcaaat gacccgatca
     3841 cgctctcgcg atcggagatg atcagccggc cgtgacagtc gccccattgc agctccgggt
     3901 cgaagccggt cagcatgccg cggcgcagat gcacaacata ggcgtcgcta cgcgtcccgg
     3961 gctcgcccgc agtgtgaatc cggccggcca catctgcttg accgtcgaag tttatctgag
     4021 ccggcgtgtc atcggcgcgc tcctccacgc tgatgctgcg cttcaccgga acgtcgctta
     4081 ccgcaatgcg cacgtcggct tcgccgctgt ccactcccac cgtgccggca atcatcgccg
     4141 ggctcccttc caggtagccg cgcaggtcct gaatttcgat gaagccctcc gcgatctcga
     4201 aatgcccggt ggcctgctcc aaccgcttcc ccggctccag caggatgctg ctgccgtcac
     4261 ccagatcagc gctgatgaag gtttccgtcg gcgggttttc cggaagttcg gggggaccgt
     4321 cctcgcgctg caaccgcacc gtgacgtcgc aactgccggt cggatcagtt tggtcccaca
     4381 accggcggta tttgcttggc agcgccgcgg ccagatcgcc atccagaggc acgttggtcc
     4441 cgctgatagt cagatcaaag ccggtcgcgt gcgcggcccg gttcacgtgg cccgtggctg
     4501 tggcatgcgc cgggccgtgg cgcgccgtga gatccaggtc caggccgcgg ggcgagaagg
     4561 tcgtccgccc gcgcgcttcg gtgaactcgt agggaaacgt gtgataccgg cagcgcacgc
     4621 cctgggcatc caaccaacct tccacgtgca gccgtccacc acccgtctct gcgtcccaaa
     4681 ctcggcgcgc ctgcaccaac aaactgaaca ggccgctcgg acgaaagtcg tttaagacgt
     4741 tgcggacgct gagcggcagc cgcggggagt caatgaacgc ggcgtcgccc ggtcgcgtcg
     4801 gaaagtgcat gccagtgacg tttaactcgg ccgagaccac gtggtcctgg gcggcggccg
     4861 gttccagcaa tgccgccaga tcggccgtca gcttgaaagc cgcgggggca cgattgacta
     4921 gccccgttcc ggtcagcttc accagcccgc gaccgttgct ctctggcgtg cagttgagcg
     4981 tcagttcggc gttgctgacc tgaagaaatc ggctccacgc gggaaccggc ggccggcctg
     5041 cctccggcac ttcgaccgga atcgagcaac gcagcccggc gaactgcaga tccaccgccg
     5101 tgagcggtaa tggcagcccg cccggcgact cagcacccga agatgactca gcgcccaaat
     5161 ctgtcctaag ggcgatacgc cgcacgcgcc cgcgcccgcg caaacccagg tcgctccacg
     5221 cctccgcgat tcggggaaag aacaccgcca gcgtgtccag ctccagccaa tcgcaggcgc
     5281 tttcgcaagt ttcgctttgc ggatcccagc gcagcgtgaa cagtggctcg cctttgtcac
     5341 cggtgcgccg caattccaga gcgtaaaccc cgtccgacca ggccccctcg ccgacgacgc
     5401 gccaatgccg aacaagctgc gcaccgcgcg gatcgctttg cgaaacgcgg atgttcgtgt
     5461 cgcccaggcg gagttccggt atcggtcttc cacgtcgcag catctgcaag atgccgcggc
     5521 cggccgacga gtttgccaag tcttctattt ccagtccggc cgtgtctccg agcgggtccg
     5581 tagccccgcc cgtctcgggc aggactacgg cgacatccgc tcggtctaac gccgcccagc
     5641 gcggaacaaa ctcccctttt agcagtgccc ccagccggca ccggatacgt gtccgttcaa
     5701 tgcgcagcac cgcccgcacg cagggcgcgt cgtcggccaa actgcccggc ccgcgcaacg
     5761 ccaggtacag accccggacc tccaggccat gccagggcgt gaatgagatg ccgtccaggc
     5821 tcacagcctc gacgggccac tcatcgaaag ccgccagaag ctgccgccgc aagcgtcctg
     5881 aattcgtgaa gtagaggtac aggccggccg tgagaaccag gccggcaatc gacagccatg
     5941 tcatcccgcg gcgccagccg ccatgcctct gcgccatatc cgaccattct ggccgactat
     6001 c
//
LOCUS       DAOSYS010000217         5937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11286_length_5937_cov_6.187011, whole genome shotgun sequence.
ACCESSION   DAOSYS010000217 DAOSYS010000000
VERSION     DAOSYS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11286_length_5937_cov_6.187011"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /gene="lnt"
                     /locus_tag="PLU99_12310"
     CDS             <1..1547
                     /gene="lnt"
                     /locus_tag="PLU99_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOO17876.1"
                     /translation="RHGISPLWTLPVAWVACEYLRARVMSGFPWLFMAHAFYEQIWFI
                     QISDLVGAYGVSFLAALVNGLLAALMLDGPGLRFGFARWRPAESLPKRVQLYVGAGVT
                     IALLALTLIYGAHRAGQQDFADGPRVAVVQHDFTLSSEPPFSETPAWVIFGEYLRLAA
                     GAATQQPDIVAFPETVWGATQNAEFLAVEHNVVSGLPTGTWFYSKLCHEAISAFARGD
                     YATVNTRFEDFERRYGGKLPRLPAHGGPPVTVLLGATSVESVPGAAYPASKRYNSALV
                     YDPDGTQRLQRYDKRHLVPFGESVPFRYGRLHWLYRWLNKLSPFSMGGEIEYTLDPGR
                     ELTVFELEYAGGTSRFGAPICYEDVMPYLCRDYVWSGGQRRVDFLINISNDAWFLHSD
                     ELPQHLAICAFRAVENRVGIARAVNTGISAFIDPNGRITAAVEKDGQRFGPGIVGCLV
                     APVKIDQRASFYGRTGDWFAVACLLATAALWLVAVTERWVLALRLRIAEWRARRGGVR
                     TDGASL"
     gene            1531..2571
                     /locus_tag="PLU99_12315"
     CDS             1531..2571
                     /locus_tag="PLU99_12315"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO17877.1"
                     /translation="MEHRYSCMAVRRGPLFALALLILAGPLACGCQTSNIAFNAVAEP
                     ELRRRAVQLLLRATQSDDVEVACNATEALVRVAPAQGLPYFRSSLRSEYPLVRFAGLV
                     ALGTLRDNVSRSEICSLLADESQLVRLAAAFAAYRCGAKQYAQVLVTALHDHPDENVR
                     AEAAHLIGLLDEPRALKRLRVAEQVPANERSKRILIQLYTSMARLGDRTALQKLIDYY
                     SQGDATSRILALQGLAELGAPESREALELRVSPDEDYLINRLIAARGLAKLGSRAGYD
                     LALSNAEFSSQNPEDPDEAVRIRANAALALGAIGDPRALPVLRKLAENGQDPRVQVAA
                     CLAICEILREPA"
     gene            2732..4417
                     /gene="ftcD"
                     /locus_tag="PLU99_12320"
     CDS             2732..4417
                     /gene="ftcD"
                     /locus_tag="PLU99_12320"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOO17878.1"
                     /translation="MSQQIVECVPNFSEGRDPAVIKQITDQIETVEGVKLLDVDPGKA
                     TNRTVVTFAGAPEPVLEAAFRAAKKAAELIDMSKHHGEHPRFGATDVCPLVPIAGITM
                     EETVVLARRLAKRIGDELQIPVYCYEYAATSEERRNLATVRAGEYEGLAEKLAKPEWK
                     PDFGPAKFVTRTGAIAVGARDFLVAYNVNLNTTSAKKANEVAMDIREMGRAKRDPVTN
                     KIIKDEHGKNVMIPGKLKAVKAIGWFIEEFGIAQVSMNLTNLNITPVHVAFDEACKSA
                     TERGLRVTGSELVGLIPLSAMLDAGRYFLRKQKRSTGVADRELIKIAVKSLGLSDLYP
                     FKPEEKIIEYALAKGEKPKKLLVAMPVRDFVEETASESPAPGGGSVSATVGALGVALG
                     AMVAALSAQKWEDRWEEFSDWAERGKVMQEELLRLVDADTEAFNAMMAAMRLPKTTEA
                     EQAARQKAMQEATKAGIEVPLRVMEKSLESMDLMKAMAETGNPNSVSDAGVGALCART
                     AVMGAFLNVRINCGGVEDKAWAEEVLTRGREMERLANEREREILAIVSTKLPA"
     gene            4685..>5937
                     /locus_tag="PLU99_12325"
     CDS             4685..>5937
                     /locus_tag="PLU99_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17879.1"
                     /translation="MPRRRLSEHSAHAPRTPRPAQPLRRTPARNWPALGLAAAVGLIV
                     LTVFWPALSSRAFYLDDKEYLLENALVQRPGWESARRFFSEVLEPSTVAGYYQPLAMV
                     SLMLDAAAAGPAAGQTESEWLRPFRRTSLLLHALNAASLVLLLAMLLRTIWPAAAAGL
                     LWGLHPLTVEPVPWIAERKTLLATFFVLWALILYVRYARVACPAHARREPASRDKGRL
                     PHGWAAYFGVLLLYVLALLSKPSALPLPLLLLILDYWPLRRLSWRTLLEKLPLLVIAG
                     LAGGLAFASQARTGAVALPWQQEAGRAALVVCHNIVFYLEKVLWPVGLSPLYVASEPL
                     SLSQPAVLAGIFGTVVLAGAIVISLRWTRVVAAGLGFFLVAVFPTLGVVAVHGAIAAD
                     RHMYLPLVGLALLAAWGLARLFERLR"
BASE COUNT          995 a         1836 c         2047 g         1059 t
ORIGIN      
        1 agcgtcacgg catctcgccg ctatggacat tgccggtcgc gtgggtcgcc tgcgagtacc
       61 tgcgggcgcg cgtcatgagc gggtttccgt ggctcttcat ggcacacgcc ttctacgagc
      121 agatatggtt catccagatc agcgacctgg tcggggccta cggcgtgtcg ttcctggccg
      181 cgctggtaaa cggcctgctg gcggcactca tgctcgacgg tccgggcttg cgcttcggat
      241 tcgcgcgttg gcggccggcc gagtcgttgc ctaagcgagt gcaactttac gtgggggccg
      301 gcgtgaccat tgcgttgctg gcgctgacgt tgatttatgg cgctcaccgg gccggccagc
      361 aggatttcgc ggacggcccg cgagttgcgg tcgtgcagca cgatttcacg ctttcgtcag
      421 agccgccctt cagcgaaaca ccggcctggg tgattttcgg cgagtacctg cggttggcgg
      481 ccggggcggc cacgcagcaa ccggatattg tggcgtttcc ggaaacggtg tggggggcca
      541 cgcagaatgc ggagttcctc gcggtcgagc acaacgtggt ttccggcctg cctaccggaa
      601 cctggttcta cagcaagctc tgccacgagg cgatttccgc cttcgcgcga ggcgactatg
      661 ccaccgtgaa tacccggttc gaagacttcg agcggcgcta cggcggaaag ctgccgagac
      721 tgccggcaca cggcgggccg ccggttacgg tgctgctggg cgcgacgtcg gtcgagtcgg
      781 ttcccggagc ggcttatccg gccagcaagc gctataactc agcgctcgtt tacgacccgg
      841 acggcacaca gcggctgcag cgctacgaca aacgccactt ggtcccattt ggcgagagcg
      901 ttccgttccg gtatgggcgg ctgcattggc tgtatcgctg gttgaacaag ctcagcccat
      961 tcagcatggg cggcgaaatc gagtacacgc tggatccggg gcgggagctg accgttttcg
     1021 agttggaata tgcgggcggc acgagccgct tcggggcgcc aatctgctac gaagacgtga
     1081 tgccgtatct gtgccgcgat tacgtgtgga gcggcgggca gcggcgcgtg gactttctga
     1141 tcaacatcag caacgacgcc tggttcctgc actctgacga gttgccgcaa cacctggcga
     1201 tttgcgcgtt tcgggcggtt gaaaaccggg tgggcatcgc gcgggccgtg aataccggga
     1261 tcagcgcgtt catcgacccg aacggacgaa tcaccgcggc ggtcgaaaag gacgggcagc
     1321 gcttcggccc cggcatcgtc ggctgtctgg tcgccccggt gaagatcgac cagcgcgcga
     1381 gcttctatgg ccgcacgggc gactggtttg ccgtggcgtg cctgctggcg acggccgccc
     1441 tgtggttggt ggcggtgacg gagcgttggg tgctggcgct gcgactgcgg attgcggagt
     1501 ggcgagcgcg gcgcggagga gttcgcactg atggagcatc gctatagctg catggccgtg
     1561 cgccgggggc ccctttttgc cttggctctg ctaatcctgg cggggccgtt ggcgtgcgga
     1621 tgccagacct cgaacattgc gttcaatgcg gtcgccgagc ccgaattgcg acggcgggcc
     1681 gtgcagttgt tgttacgcgc tacgcagagc gatgacgtgg aagtggcctg caatgcgacg
     1741 gaggccctgg tccgggtcgc cccggcgcag ggcttgccat acttccggtc gtcgctgcgc
     1801 tctgaatatc cgctggtgcg gttcgccggg ctggtcgcgc tgggcacgct gcgcgacaac
     1861 gtaagccgca gcgagatctg ttcgctcctg gccgatgaga gccagttggt tcgcctggcg
     1921 gccgcgttcg ccgcttaccg ttgtggcgcg aagcagtacg cgcaggtgct ggtcacggcg
     1981 ctgcacgacc atccggacga gaatgtgcgg gccgaggcgg cgcacctgat tgggctactg
     2041 gatgagccgc gggcgttgaa gcggctgcgc gtggcggagc aagtgccggc caacgagcgc
     2101 tccaagcgca tcttgataca gctttacaca tccatggcgc ggctgggcga ccggacggcc
     2161 ttgcagaagc tgattgacta ctactcgcag ggggacgcca cgagccgcat cctggcattg
     2221 caggggctgg cggaactggg tgcgccggag tcgcgtgagg cgttggagct gcgggttagt
     2281 ccggatgaag actacctgat taaccgcttg atcgcggcgc gtgggctggc gaagctcggg
     2341 tcgcgggcag gttacgactt ggccttgtca aacgccgaat tctcatctca gaaccctgaa
     2401 gacccggacg aggcggtgcg catccgggca aacgcggcgc tggcgctggg ggcaatcggc
     2461 gatccgcggg ctttgccggt gctgcgcaag ctggcggaga acgggcagga tccgcgcgta
     2521 caggtagcgg cgtgcctggc aatttgcgag atactgaggg aaccggcgta ggggtgacag
     2581 agtgataggg tgataaagtg atagggtgat aaggcgattc cgcgcgccct cctttatctc
     2641 actttatcac cctatcaccc gagcgacttc tcaccgtccg ccttttcctg ttaccatgcc
     2701 ccgtttctcg aacaccgcag gaatggagca tatgagccag cagattgtcg aatgcgtgcc
     2761 gaacttcagc gagggccgcg acccggccgt catcaagcag attactgacc agattgagac
     2821 cgttgagggc gtgaaactcc tcgacgttga ccccggcaaa gcgaccaacc gaacggtcgt
     2881 caccttcgcc ggcgcgccgg agccggtgct cgaagccgcg ttccgcgccg ccaagaaagc
     2941 ggccgagctg atcgacatga gcaagcacca cggcgagcac ccgcgtttcg gcgccaccga
     3001 tgtctgcccg ctggtgccca tcgccggcat caccatggaa gagacggtcg tgctggcccg
     3061 ccgcctcgcc aaacgcatcg gcgacgagct gcaaatcccg gtctactgct acgaatacgc
     3121 cgcaaccagc gaagagcgcc gcaacctcgc gacggtgcgg gccggcgagt acgagggcct
     3181 ggccgagaag ctcgccaagc cggagtggaa acccgacttc ggcccggcca aattcgtcac
     3241 gcgcaccggc gcaatcgcgg tcggcgcccg cgacttcctg gtggcctaca acgtcaacct
     3301 caacacgacc tcggccaaga aggccaacga ggtcgccatg gacatccgcg agatgggccg
     3361 ggccaagcgc gacccggtca cgaacaaaat catcaaggac gagcacggca agaacgtgat
     3421 gatccccggc aagctgaagg cggtgaaggc catcggctgg ttcatcgagg agttcggcat
     3481 cgcccaggtc tcgatgaatc taaccaacct gaacatcacg ccggtgcacg tcgcgttcga
     3541 tgaagcctgc aagagcgcga ctgaacgcgg tctgcgggtg accggctcgg agctggtcgg
     3601 gctgattccg ctcagcgcca tgctcgatgc cgggcgctac ttcctgcgaa agcagaagcg
     3661 ctcgaccggc gtcgccgatc gcgagctgat caagatcgcg gtcaagtcgc tcggcctcag
     3721 cgacctgtat cccttcaagc cggaagagaa gatcatcgag tacgccctgg ccaagggcga
     3781 gaagcccaag aagctcctgg tcgccatgcc ggtgcgcgat ttcgtcgagg aaaccgcctc
     3841 ggaatcgccg gcgccgggcg gcggctcggt atccgcgacg gtcggcgcgc tgggcgtagc
     3901 gctcggggcg atggtcgcgg ccctttcggc ccagaagtgg gaagaccgat gggaagaatt
     3961 ttcggactgg gccgagcgcg gcaaggtcat gcaggaagag ctgctgcgcc tggtcgatgc
     4021 ggacaccgag gcgttcaacg ccatgatggc cgccatgcgc ctgccgaaga cgaccgaagc
     4081 cgaacaggct gcccgccaga aggccatgca ggaggccacc aaggccggca tcgaagtccc
     4141 gctgcgcgtc atggaaaagt cgcttgagtc aatggacctc atgaaggcca tggccgaaac
     4201 gggcaacccg aactccgtgt cggacgccgg cgtgggcgcg ctttgcgctc gcacggcggt
     4261 catgggcgcg ttcctcaacg tgcgcatcaa ctgcggcggc gtcgaggaca aggcttgggc
     4321 ggaagaggtc ctgactcgcg gccgggagat ggaacgtctc gcaaacgagc gcgaacggga
     4381 aatcctcgcg atcgtcagca ccaaactccc ggcgtaacga tgcgctcgca tccgagccgt
     4441 atcgccaccg cagaacgaag tcgtagcaga ctcagcgcgc ggagagggac cgaggaatca
     4501 aggacggcac attcccacct tcccacattc ccacgttccc accttcccac agacatgcgt
     4561 gggaaagtgt gaacgtgtga acgtgggaat gggaggccct cctgatccga gcccgaagcg
     4621 caagcgaggg caaccctatc cgcgcggttg gcaatccgcg cctatcccag gcagaatagg
     4681 gccgatgcca cgccgccggc tgtctgaaca ttcggcccac gcaccgcgga cccctcgccc
     4741 cgcgcagccg ttacgccgca cgcccgcgcg aaactggccc gctttgggct tggcagcggc
     4801 ggtcggccta atcgtgttga cggtgttctg gccggcgcta tcgagccgcg cgttctacct
     4861 ggacgacaag gaatacctgc tggaaaacgc gctggtgcag cggcctgggt gggaatccgc
     4921 gcggcgcttc ttcagcgaag tgttggaacc ctcgaccgtc gccggctact accagccgct
     4981 ggccatggtc tcgctcatgc tcgacgcggc tgctgcggga cctgcggccg gccagaccga
     5041 aagcgagtgg ctgcgcccgt tccgccgcac cagcctgctg ctgcacgcgc tgaacgcggc
     5101 tagcctggtg ctcttgctgg caatgctgtt gcgcacgatt tggccggcag cggcggcggg
     5161 cctgctgtgg ggtctgcacc cgttgacggt cgagccggtg ccctggattg cggagcgcaa
     5221 gacgctgctg gcgacattct tcgtgctgtg ggcgctcatc ctgtacgtgc gctatgcgcg
     5281 cgttgcgtgc cctgcccacg cgcggcgcga gcctgcgtcg agggacaaag gccgactacc
     5341 ccacggctgg gccgcatatt tcggcgtact gctgctttac gtgttggcgc tgctgtccaa
     5401 gccgagcgct ttgccactac ctttgttgtt gctgattctc gattactggc ctttgcggcg
     5461 tttgtcgtgg cggacgctgc ttgagaagct gcccctgctg gtgatcgccg gtttggcggg
     5521 cggcctggca ttcgcgtcgc aggcgcggac cggcgccgtc gccctgccgt ggcagcagga
     5581 ggccgggcgg gctgcgctcg tggtttgcca caacatcgtc ttttaccttg agaaagtgct
     5641 gtggccggtg gggctgtcgc cgctgtacgt cgcgtcggag ccgcttagct tgtcgcagcc
     5701 ggccgtattg gcgggaatct tcgggacggt cgtattggcg ggggcgatcg tgatctcact
     5761 tcgctggacg agggtcgtcg cagccggcct ggggttcttc ctggtggcgg tattcccgac
     5821 gctgggggtg gtggcggtgc acggcgccat cgcggccgac cggcacatgt acttgccgct
     5881 ggtgggattg gcgctgttgg ccgcctgggg cctggcacgg ctgtttgagc ggctgcg
//
LOCUS       DAOSYS010000218         5936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11289_length_5936_cov_4.893215, whole genome shotgun sequence.
ACCESSION   DAOSYS010000218 DAOSYS010000000
VERSION     DAOSYS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_5936_cov_4.893215"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLU99_12330"
     CDS             complement(<1..629)
                     /locus_tag="PLU99_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOO17880.1"
                     /translation="MRLLAIGDIVGRPGKHACSQLIPRLLAERGIDFVVANSENASDG
                     SGLTPAMFAKLRHYGIDVCTMGDHIYRRREIMPLLESEERIVRPANLPPEATGRELAL
                     VTARSGERVAVISLLGRTYMNVRSDCPFHAADRVLAAVPHDVKVIVVDVHAETTSEKL
                     ALGWYLDGRVSAVVGTHTHVQTADETILPKGTAYITDLGMTGPHDSVLGR"
     gene            complement(845..1429)
                     /locus_tag="PLU99_12335"
     CDS             complement(845..1429)
                     /locus_tag="PLU99_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOO17881.1"
                     /translation="MAARSPTKPPSIHDASNRLQIKKYPNRRFYDSTRGQNVSLADLH
                     DLIVQGHELAIVDSTTGEDITNQILTQIILERHAPKLSIFPAAILHQIIRTQEQFLGS
                     VLEQFFRQTLEAQRAAHAQWAKMWQSAFNLPAAAMPSAADWTRVWLEAAGAANRAAGP
                     SAPEAPKAGTEVEELRQQISALMKRVDELQSRKK"
     gene            1743..2174
                     /locus_tag="PLU99_12340"
     CDS             1743..2174
                     /locus_tag="PLU99_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17882.1"
                     /translation="MTAAATPPNVFDAMKQVAENCVKAMQAGMKFYEDTAQIYTEMAT
                     KNIDQAKDGYVRLANDLSDLNRKNIERFQSFVDEQVRRNASLAPYMVDPAAFTNPAEM
                     YDRMAALWRNSFQGLRDSVTTTAKVITDAAEGWTRVYRTAQ"
     gene            2279..2722
                     /locus_tag="PLU99_12345"
     CDS             2279..2722
                     /locus_tag="PLU99_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17883.1"
                     /translation="MAGESPFSMPSLNPFLAFWNDFYTKMVAAGLAPGKDPQDAAEQM
                     RRSFFDAMSQYADEFLRSEVFLKAMKQSFDNVVAMQGATKQYLQKGLAAAQMPSLVDQ
                     EQVVGMIRGTEDRLFTRLDEIQSRIERLEKALENAAEKRPRTKTS"
     gene            2762..3865
                     /gene="phaC"
                     /locus_tag="PLU99_12350"
     CDS             2762..3865
                     /gene="phaC"
                     /locus_tag="PLU99_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HOO17884.1"
                     /translation="MATVTQIPWFLNPESYLQPWQQAIAKSLAIPKVIDVMRRTRVGA
                     TPTRPVYREDGLRLLEYETEAPRKFKTPLLLVFALVNRSYIVDLRPGKSVIHHFLQGG
                     FDTYNVDWGKPTPSDTHLALDDYVLGYLDHVVDFVRRKCEVDKLNMLGYCMGGTMAAI
                     YAALRPEKIKNLILMAAPVDWSAKANLLSVWSDERYFDVDKLVDAYGNAPAEWLQASF
                     LLLRPFQNTVEKWMRFYEKMDDENYLEDFFAMETWLNDNIPVAGEVFRQFVKYMFQKN
                     MLIQGKLEVGGERVDLKKIACPILNLTAQHDHLVPPNQSLPFNDAVSSTDRKSINFPV
                     GHIGLAVSSKSHRDLWPQVINWLGQRSDAHRPH"
     gene            3877..4755
                     /locus_tag="PLU99_12355"
     CDS             3877..4755
                     /locus_tag="PLU99_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO17885.1"
                     /translation="MSSGPNTKQRTNSIEETLPTQLDLRTRVAMITGGAGGIGRATCL
                     ELARKGIRAVGVVDLSPGVDDFCLDGNKRIGREVLIPYCGDVTEPGFRRSAFADLERR
                     FGTVTVCIPAAGITRDRLAVRINKETGEPDIYNEEEFRRVMAVDLVAPVYWALETIAS
                     VARDRHNKGLKKWTPSEGVQGAIIFIGSVSSSGNRGQVAYASAKAGLDGAQATLATEA
                     IFHGVRCAIIHPGYTDTPMVRALGDEFIATRILPFTQLGRLIHPAEIAHAIAFLVCNS
                     AVSGQLWADAGWHPVA"
     gene            5153..>5936
                     /locus_tag="PLU99_12360"
     CDS             5153..>5936
                     /locus_tag="PLU99_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO17886.1"
                     /translation="MAAEAVVTSNETYAKTGSDLLAATGTKTPTAPQERKRPTTGQLT
                     GKVALVTGGSRGIGRAICQALAGEGADVAVNYVRNDEGAQQTLRLVTGHGVHGSVYKG
                     NVGSNDDIHRMIEEILKDFGHIDILVNNAGITRDKSFAKMTQDVWREVVDVDLTGQAM
                     ITQEVLRHMAGYGWGRVIFITSLVGQMGNFGQANYAVAKGGLIALVKTLAREFSRKGI
                     TVNAVAPGYIETDMISAVPQNVLAAVCQMTPIGRLGKPEEVAE"
BASE COUNT         1203 a         1769 c         1873 g         1091 t
ORIGIN      
        1 cgccccagca ccgagtcatg cggcccggtc atgcccaggt cagtgatgta cgccgtaccc
       61 ttggggagga tcgtctcgtc cgccgtttgc acgtgcgtgt gtgtcccgac cacggcggaa
      121 actcggccgt cgaggtacca gcccagcgcc aacttttcac tcgtcgtctc ggcgtggacg
      181 tccaccacga tcaccttcac gtcatgcggc acggcggcca gcacccggtc agccgcgtgg
      241 aacgggcaat cggaacgcac gttcatatac gttcgcccga gcagcgaaat gacggccaca
      301 cgctcgccgc tccgggccgt caccagcgcc agctcgcggc cggtcgcctc gggcggcagg
      361 ttggccgggc gcacgatgcg ttcctcggat tccagcagcg gcatgatctc gcgccgccga
      421 taaatgtggt cgcccatggt gcagacgtcg atgccgtagt gccgcagctt ggcgaacatg
      481 gcgggcgtca aaccgctgcc gtccgaggcg ttctcggagt tggcgaccac aaagtcgatc
      541 ccgcgctcgg ccagcagccg cggaatgagc tgtgaacaag cgtgtttacc gggccggccg
      601 acaatatcgc cgatcgcgag caggcgcatg acgaggcttt ctagcgcgag gctattcacg
      661 ggagccccgc gagcgctggc gcagtatatc acgtgtcggg gcgatcgaat actgcaactt
      721 tgctatcggg gccaaggagc cggcagcggg ggcagcccag gaagacgggg gggctgcagg
      781 cgactggcgc tgcagggggg ccgaagcggg aagcgtgcgt ctcagccggg gaggctcgcg
      841 ccggctactt cttccgcgat tgcagttcgt cgacgcgctt cattaaggcc gaaatctgct
      901 gccggagttc ctcgacctcg gttccggcct tcggcgcctc aggcgcggac ggccccgctg
      961 cccggttggc cgcgccggcc gcttcaagcc agacgcgcgt ccaatccgcc gcggagggca
     1021 tggccgctgc gggcagattg aaggccgatt gccacatctt ggcccactgg gcgtgagcag
     1081 ctcgctgggc ctcgagcgtc tggcggaaga actgttcgag caccgaaccc aggaactgtt
     1141 cctgcgtacg aatgatctgg tgcaggatgg ctgcggggaa aatcgagagc tttggcgcgt
     1201 ggcgctcgag aatgatctgg gtcaggatct ggttcgtgat gtcttcgccg gtcgtggagt
     1261 cgacgatcgc aagttcatgc ccttgaacga tcagatcgtg caaatccgcc agcgacacgt
     1321 tctggccgcg cgtcgaatca tagaaccggc gattcggata tttcttgatc tgcagccgat
     1381 tgcttgcgtc gtggatggac ggcggttttg taggcgaacg agcagccata ggctggatag
     1441 cttacagtcc ggacgcccgt agtgcaataa caaaaaagtt aatccggaat gcccgactcg
     1501 gatagcgcaa ctctctcaaa ataaaggctt tctctgcctc gccacatttt tgtgcaatcg
     1561 cacaaattta acttgcgatt ctgccaaagc gcgggtatgt ttcttcacga acggactgct
     1621 tggccgggcg gccgcagagt gcggcgcggc ctcaggttgc ggtccgctac ggtccccgga
     1681 tggcgcgcaa gaaggacaac ccggcgagag ccggcagacg aaagacccaa aaggagccac
     1741 tcatgacagc cgctgcgacc cccccgaacg tgttcgatgc gatgaaacag gttgccgaga
     1801 attgcgtcaa ggccatgcag gccggcatga agttctacga ggacacggcg caaatctaca
     1861 ctgagatggc gaccaagaac atcgaccagg ccaaggacgg gtacgtaaga ctcgctaacg
     1921 acctctccga cctcaaccgg aaaaacatcg agcgcttcca gtcctttgtg gatgagcagg
     1981 tccgccgcaa cgcttcgctg gccccttaca tggtcgatcc ggccgccttc accaacccgg
     2041 ccgagatgta cgaccggatg gccgccctgt ggcgcaactc cttccagggc ctgcgcgact
     2101 cggtcactac gaccgccaag gtcatcactg atgcggccga aggttggacg cgcgtttatc
     2161 gcacggccca gtagccgcgg aggccaccgc cgccgcgagc agtgacagcg gccgaaatag
     2221 cgctggcgcg cggcggcgcg cctcgcgatt tgcttacccg agaagctgga ggtgacgctt
     2281 ggctggcgaa tcgccttttt cgatgccgtc gttgaacccc ttcctggcgt tctggaacga
     2341 cttctacaca aagatggtgg ccgcgggtct ggcacccggg aaggacccgc aggatgcggc
     2401 ggagcaaatg cgacgttcgt ttttcgacgc gatgtcgcaa tatgcggacg agttcttgcg
     2461 cagcgaggtc ttcctcaaag cgatgaagca gtcgttcgac aacgtcgtgg ccatgcaggg
     2521 cgcgaccaag cagtatttgc agaagggcct ggcggcagct cagatgccct cgctggtcga
     2581 tcaggagcag gtcgtcggga tgatacgcgg tacagaagat cgtcttttta cgcggctcga
     2641 cgagatccaa tccaggattg aaaggctcga gaaagcgttg gagaacgcgg ccgaaaagcg
     2701 cccccgcacg aaaacgtcgt gaaattgacc ggccacaacg ccgccggcga ggcacaagat
     2761 aatggcgact gtgacgcaga taccatggtt cctgaacccc gaatcctacc tgcagccttg
     2821 gcagcaggcg attgccaaat cgctggcaat tccgaaggtg atcgacgtaa tgcgccgcac
     2881 gcgggtcggc gccacgccca cgcgccccgt ctaccgggaa gacggtttgc gcctgctcga
     2941 atacgaaacc gaagcaccac gcaagttcaa gacgccgctg ttgttggtct tcgcgctcgt
     3001 gaatcgctcc tacatcgtcg acctccggcc cggcaaaagc gtgatccacc acttcctgca
     3061 gggcgggttt gatacttaca acgtggactg gggcaaacct acgcccagtg atacgcactt
     3121 ggcgctggat gattacgttc tcggctatct ggaccatgta gtcgatttcg tgcggcgaaa
     3181 gtgcgaggtc gacaagctga atatgctcgg ctactgcatg ggcggcacca tggccgccat
     3241 ttacgccgcc ttacggccgg agaaaatcaa gaacctgatt ctcatggccg ctccggtgga
     3301 ctggtcggcc aaagcaaatc tgctgtcggt gtggtcagat gagcgctatt tcgacgtcga
     3361 caagctggtg gacgcttacg gcaacgctcc cgccgagtgg ctccaggcgt cgtttctgct
     3421 cctacggccg ttccaaaaca cagttgagaa atggatgcgg ttctacgaga agatggacga
     3481 cgagaattac ctggaggact tcttcgcgat ggaaacctgg ctgaacgaca acatcccggt
     3541 tgcgggtgag gtcttccgcc agttcgtcaa gtacatgttc caaaagaaca tgctgatcca
     3601 gggcaagctc gaagttggcg gtgagcgcgt cgacctgaag aagatcgcgt gcccgatcct
     3661 caacctcacc gcgcagcatg atcatctcgt gcctcccaac caaagcctgc ccttcaacga
     3721 cgccgtgtct tcaacagatc gtaaatcgat caatttcccg gtcggccata ttggcctggc
     3781 cgtttccagt aagtcgcacc gcgacctttg gccgcaggtg atcaattggc tgggtcagcg
     3841 ctccgacgct caccggccgc actaaggggc acagctatga gttcgggccc gaacaccaag
     3901 cagcgcacga attcaattga ggagacgttg ccgacgcagc ttgatctgcg gacgcgggtc
     3961 gcaatgatta ccggcggcgc gggcggcatc ggccgggcca cgtgcctgga attggcccga
     4021 aaagggatac gcgccgtcgg tgtcgtcgac ttgagtcccg gcgtcgatga tttctgcctt
     4081 gacggcaaca agcgcatcgg ccgggaggtg ctgatacctt actgtggcga tgtgaccgag
     4141 cccggctttc gccgcagcgc atttgccgac ctcgaacggc gctttggcac ggttactgtc
     4201 tgcattccgg cggcgggcat tacgcgcgac cggctcgctg tgcgaatcaa caaagagacc
     4261 ggcgagcctg atatttacaa cgaggaggag ttccgccgcg tcatggcggt cgacctggtt
     4321 gctccggtgt attgggcgtt ggaaacgatt gccagtgtgg cccgcgatcg ccacaacaag
     4381 ggcctgaaaa agtggactcc cagcgagggc gtacagggcg caatcatttt catcgggtcg
     4441 gtctcatcgt cgggaaaccg tgggcaagta gcgtatgctt ccgccaaagc cgggctggat
     4501 ggcgctcaag ccactttggc gacagaagcg attttccacg gcgtgcgctg cgcgatcata
     4561 cacccgggct atacagatac gccgatggtc cgggcactgg gcgacgagtt tatcgccacc
     4621 cggattctcc catttacgca gctcgggcgc ctgattcacc cggctgaaat tgcgcacgcc
     4681 atcgcattcc tggtgtgcaa cagcgcggta agcgggcaac tctgggcgga cgcgggctgg
     4741 catcccgtcg cctgacgaaa cggcaaaatt gtcagtaaga tgattcgctg aaggactgca
     4801 ttccgtaatt gcggatatgg cgagtcgcca gtctctgcag ttgcaattcg atgcgaggtg
     4861 tgcgtaacag actgccttca caatacccaa gtgtgcacgt cgtcattgct tcaccagtgc
     4921 gccgaccctg caatgctcgc cagccaagtc cggcacacgt aagagtttat cgccaataaa
     4981 ggtgaattaa ttggcccagc gcgactgccg ggtgctaagc tcatgtgtgt ttttctggtg
     5041 tgcgcgagtg aatcggccgc tctgcaacgg gcgcagccgc agcttagtct gcgtgcctcg
     5101 ccggggcgga ctgacgggtc gccattaatc gggcattaga ggcaggtggg ttatggccgc
     5161 ggaagcagtc gtcacttcga acgaaaccta tgcgaagacg ggcagcgatt tgttggcggc
     5221 aacgggcacc aagacgccaa ccgcgccgca ggaacgaaag cgccccacca cggggcagct
     5281 cacgggaaag gtggcgctgg tgaccggcgg ctcgcgcggc atcggtcgag ccatctgcca
     5341 ggcgctggcg ggggaggggg ccgacgtagc cgtaaactac gtgcgcaacg acgaaggagc
     5401 gcagcagacg ctgcgcttgg tcaccgggca tggggtccac ggcagcgtct ataagggcaa
     5461 cgtgggcagc aacgacgaca tccaccgcat gatcgaggag attctcaagg acttcggcca
     5521 catcgacatt ctcgtcaaca atgccggaat cactcgcgac aagagcttcg cgaagatgac
     5581 gcaggacgtg tggcgcgagg tggttgatgt cgatctgacc ggtcaggcga tgatcacgca
     5641 ggaggtgctc aggcacatgg ccggctacgg ttgggggcgg gtgatcttca tcacctcgtt
     5701 agtcgggcag atgggcaact tcggccaggc caattacgcc gtcgccaagg gcggtctgat
     5761 cgcactggta aagaccctgg cgcgcgagtt cagtcgcaag ggcatcacag tcaatgctgt
     5821 cgccccgggc tacatcgaga ctgacatgat ttccgccgtg ccacagaacg tgcttgcggc
     5881 ggtctgccag atgacgccga tcggacgcct cggcaagccc gaagaggtgg cggaag
//
LOCUS       DAOSYS010000219         5891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11390_length_5891_cov_3.746744, whole genome shotgun sequence.
ACCESSION   DAOSYS010000219 DAOSYS010000000
VERSION     DAOSYS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11390_length_5891_cov_3.746744"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="fmt"
                     /locus_tag="PLU99_12365"
     CDS             complement(<1..823)
                     /gene="fmt"
                     /locus_tag="PLU99_12365"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOO17887.1"
                     /translation="MRIVLLGTADFAVPALRALVTAGHDITAVISQPDRPAGRGRTLA
                     PTPMHAAADELGLRHIQAEDVNAPHLADLFAPGPSQPEIGVVAAFGQKIGPAILSALP
                     RGCINIHGSLLPKYRGAAPYQWAIINGETTTGVTIFQLEQRWDAGPIWSQRETPIGET
                     ETADELHDRLALLGAELLIETLADIQAGRVQPRPQDAAQSTRAPKLTKADAALDWNQP
                     AAKIVRRIHGLWSWPAATCTFVSRSGKRERVQLARARVVDPDAPPAPATGAETTIA"
     gene            complement(1649..4366)
                     /locus_tag="PLU99_12370"
     CDS             complement(1649..4366)
                     /locus_tag="PLU99_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17888.1"
                     /translation="MVTISLCGSGDENDTVVWVYRGNCDGYPIAWNDDSPECGNLNSK
                     IENIPMPAGTTYYIVIAGYGYSTVDYVLNVTSVPLVTGACCFCDATCMETCESDCTDR
                     AGTYQGDGTYCWSSECTYQPPPNDDCESVTPVTLSNGVPEVFTGHNACSTNDCGMVSG
                     PQVWIAFTITELSDVDISYCGTWPTQTTVSVWMFDACPCDADYYAATTSGWDCPDGSV
                     NFHGTWKGLAPGTYYYGVQTAPGAEGPYYVTVTATVPVYGACCLCDGACAVVMEEDCA
                     AEFGAYYGDNTTCEQAGCTPVPVPENNNCHNVQPVTLLDGVPQQFTGTTRCAMAADCS
                     LLMYPTVWHAFTLDWPAQVTVEFCGTSPAFENAYWTLMSGCPCNSGVIRDTFSWKACG
                     DGNLTFGFNGLATGTYYLPVLAAPIWASGPYVVTVTATPVAGGACCVCDGTCRDGTAE
                     SCATLDGLYYGDGSSCEDTECPNQIPPNAYCEDVTPVPVTPGVPLVFEGNSHCGPQSC
                     SILGAQGAVWVAFTLSHETDVTIAFCGTNPRFGTAYTTLMTACPCANPIWANDYNWNS
                     CGVGDTNVTLFFYDVPAGTYYYPVPYLPASDANGPYVVTIITPAPTGGCCMETGCAVM
                     TQPDCAAQGGWYVGDYTDCGGVSYNVNQGTAFEDISATGVEFSPAETDDGGGEYALPF
                     TFNFYGEDHTTVGIASNGYLTFSTDWSTTGGAWTFPDPTPPNNMIAAFWTDLVPAPET
                     APVHVATLGTEPNRRFIVQFTNVPIWWFGGSNTFQIVLFETTNNIEFRYQNFDAWFYR
                     WIEAGIENANGTVGIELHPQVQPNTSVAITYNVAPSPCARGDMNCDGLLNAFDIDPFV
                     LALVTPEQYAAAFPDCLIERADCNCDGIVNAFDIDAFVQCLLGGCQPCP"
     gene            4390..5640
                     /locus_tag="PLU99_12375"
     CDS             4390..5640
                     /locus_tag="PLU99_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17889.1"
                     /translation="MTDSVSSKCADIDVHEQQPLELRKQVESVMLRLRAAFAGILESV
                     PTRNGRPHEVAELLKLDKQLVWKISRLVKTRDPVMMARHLPGPTAIRRFLDAAQLHVA
                     PGVIEAIHAPLEDLNELIRVHAGDRATFEMLLSEWCSREPDAAVTHRREAFRGNSYIW
                     GVQARTKLTSMFLHPSSEDGVFDIAIVAGVVDLRRLRLNLPWVIARWAVSDNDGEVRF
                     NADREPLDPESNDPLLRRFCSKPLPQLRIVNGAPGMLEYQLAPGAIGNSGAVTCIYGN
                     IVRRVASCYRDEHNEFNEVMAAMSTPCEVLVLDQIVHESLFGRIKPDLLVYSGLNASE
                     QYPRGVTRVQLPVAEQVEYLGKGPAVLPTPHVPRYRELAEYVFERTGWDGSRFDVYRA
                     EMRYPPIPSTVVMQHALRERPAGR"
BASE COUNT         1075 a         1746 c         1989 g         1081 t
ORIGIN      
        1 gcgcgattgt tgtttcggcg cctgttgcgg gcgcaggcgg tgcatccggg tcgacgacgc
       61 gggcgcgggc cagttgcacg cgttcgcgtt tgccgctgcg cgagacgaac gtgcaggtcg
      121 cggcgggcca ggaccacagg ccatgaatgc ggcgcacgat cttcgcggcg ggctggttcc
      181 agtcgagggc ggcgtcggct ttcgtcagct tcggggcgcg cgtgctttgc gcggcgtcct
      241 gcgggcgggg ttggacgcgg ccggcttgaa tgtcggcgag ggtctcgatc agtaactcgg
      301 cgccgagcag ggccaggcgg tcgtggagtt cgtcggcggt ctcggtttcg ccgatgggcg
      361 tttcgcgctg gctccagatc gggccggcgt cccagcgctg ttcgagctga aagatggtca
      421 cgccggtcgt cgtttcgccg ttgatgatgg cccattgata cggggccgcg ccgcggtact
      481 tcggcaggag cgagccgtgg atgttgatgc agccgcgcgg cagggcgctg agaatggcgg
      541 ggccgatctt ctggccgaag gcggccacga cgccgatctc gggctgcgat ggtccggggg
      601 cgaagaggtc cgccaggtgc ggggcgttga cgtcttcggc ctgaatgtgg cgcaggccga
      661 gttcgtccgc tgcggcgtgc attggcgtgg gagccagcgt tcggccgcgg ccggccgggc
      721 gatcgggctg gctgatgacg gcggtgatat cgtggccagc ggtcaccagc gcccgcagcg
      781 cggggacggc gaagtctgca gtgccaagca acacgattcg catgcgcgag ttctagcggc
      841 tgggaggtta ttaggctacg gggctgttgg gcaatttggg ggggagggga aaggtggaca
      901 gccgggaaga gatcggcggc agagcgtgcg cgagacggat ttgcgcgcga gcggcggcgg
      961 cgcgcggcgg cgttaaccca gccgctgcgc ggagagaggt gagaggagtg aggagaaagg
     1021 tgacggccgc gaacgggtcg tcggcagcgt gcgcgacgga cccgcgcgcg agcggcggcg
     1081 gttccgcgac cctaggtgct caagcgatct gataaaaagc ggtgtgggat cgggtcttgg
     1141 ggggcaggtg atcaacaatt gctggccaac ctgcccgaca cgccggtcag ccgcacgacg
     1201 agtggctgcc ggacgagtgg aagaaaaggc aagcccagac gccaaagcat cccccacagc
     1261 ggattgaact ggatgacgct cacacactga gcctccgccc accggttggg ccagtgcggt
     1321 tcgttcaacg ggaacgttac acgcctcaca ccggccggga ttcccgaacg cgcggacgag
     1381 ccctatgcca ttacgggcgc catcacaaac ccgtggtcgg gcgccttcgc acgcggcacg
     1441 atacagtccc agtgtggttt cgacgccgtc ctcgaacatc tcgcgcggca aaacggaaac
     1501 attctgcccg tgtgtcctta cccttcgcat ctgacgcgcg gcaacgcctg attggggcag
     1561 aggaaaagca cagcggccga cccgatcgag cgtcgggtcg gccgctgatt gtcaggagct
     1621 tccaggtcag gctacgagcg taccggtgtt acgggcacgg ctggcagcca ccgaggaggc
     1681 actggacgaa tgcgtcgatg tcgaaagcgt tcacgatgcc gtcgcagttg cagtcggccc
     1741 gctcaatcaa gcagtcggga aatgctgccg cgtactgctc gggcgtaaca agggccagga
     1801 cgaacggatc gatgtcgaag gcgttaagca ggccgtcgca gttcatatca ccccgagcac
     1861 acgggctcgg tgcgacgttg tatgtaattg cgactgacgt attgggctgc acctgcggat
     1921 gcagctcgat tcccacggtg ccgttcgcgt tttcgatacc agcttcgatc cagcggtaga
     1981 accacgcatc aaaattctgg tagcggaact cgatgttgtt ggtggtttca aacaatacaa
     2041 tctggaacgt attgctgccg ccgaaccacc agatcggcac gttggtgaac tgcacgatga
     2101 agcggcggtt cggttccgta ccgagcgtcg ctacgtggac cggggccgtt tcgggcgccg
     2161 gaaccagatc tgtccagaac gcggcgatca tgttattcgg cggggtgggg tcggggaagg
     2221 tccaggcgcc ccccgttgta gaccagtcag tgctgaacgt cagatagccg ttcgacgcga
     2281 ttccgacggt cgtatggtcc tcgccgtaga aattgaacgt gaagggcagg gcatactcgc
     2341 cgccgccgtc gtctgtttca gccgggctga actccacgcc tgtggctgaa atgtcctcga
     2401 aagccgtacc ctggttcacg ttgtaggaga cgccgccaca gtcggtgtag tcgccgacat
     2461 accagccgcc ctgcgcggcg cagtcgggtt gggtcatgac tgcgcaaccc gtctccatac
     2521 agcatccacc ggtgggcgca ggcgtgatga tggtcacaac gtagggaccg ttggcgtcgc
     2581 tagcgggaag gtacggcacc gggtagtagt aggtgccggc cggcacgtcg tagaagaaga
     2641 gcgtcacatt ggtgtcgccg acaccacaac tgttccagtt atagtcatta gcccagatgg
     2701 ggttcgcaca cggacacgcg gtcataagcg tggtgtaagc cgtaccgaag cgcggattgg
     2761 tcccgcaaaa tgcgatggta acatccgttt catgactgag cgtgaaggct acccagaccg
     2821 cgccctgcgc ccccaggata gagcagctct gcggcccgca atggctgttg ccttcgaaca
     2881 ccagcggcac gccgggcgtc accgggaccg gcgtcacatc ctcgcaatag gcgttcggcg
     2941 ggatttggtt cgggcactca gtgtcttcgc agcttgagcc gtccccgtag tacaggccat
     3001 ccaacgtggc gcaagactcc gcagtgccgt cgcggcaggt gccatcacaa acgcagcacg
     3061 cgccgcccgc gacgggcgtg gccgtgacgg tcacgacgta aggcccgctg gcccagatcg
     3121 gggcagccag aaccggcagg taataggtcc cggtcgccag cccgttgaag ccgaacgtga
     3181 ggttgccgtc gccacaggcc ttccagctaa aggtgtcacg tatgacgccg ctgttacagg
     3241 gacagccact catcagggtc caataggcat tctcgaacgc gggagaggtg ccgcagaact
     3301 ccaccgttac ctgggcgggc caatcgagcg tgaaggcgtg ccacacggta ggatacatca
     3361 gcagcgagca atcggccgcc atcgcgcagc gggttgtgcc cgtaaattgc tgcgggacgc
     3421 catccagaag ggtgaccggc tggacgttgt gacagttgtt gttttcaggg acaggtacgg
     3481 gcgtgcagcc ggcctgctcg caggtcgtgt tgtcgccgta gtaggcgccg aactcggcgg
     3541 cgcagtcctc ctccatcact acggcgcagg cgccgtcgca caggcagcaa gcgccgtaaa
     3601 ccggcacggt ggccgtgacg gttacgtagt aaggcccctc ggcgcccggc gcggtctgca
     3661 ccccgtagta ataggtgccg ggtgccagcc ctttccaggt gccatggaag ttaacgctgc
     3721 cgtcggggca gtcccagccg ctcgtcgtgg cagcgtagta atccgcgtcg caggggcagg
     3781 cgtcaaacat ccagaccgat acagtagtct gggtcggcca ggtcccgcag taactgatgt
     3841 cgacgtcgga taattccgtt atggtgaagg cgatccagac ctgcggaccg ctgaccatcc
     3901 cgcaatcgtt ggtcgagcac gcattatgcc cggtgaacac ctccggcacg ccattcgaca
     3961 aggtaacggg agtgacactc tcacagtcgt cgttgggcgg cggctgatat gtgcactccg
     4021 agctccagca gtacgtcccg tcgccttgat acgtgccagc ccggtcggtg caatcggact
     4081 cgcaggtctc catgcacgtg gcgtcgcaga agcagcaggc cccggtcacg agcggcacgc
     4141 tggttacgtt gagaacgtag tccaccgtgc tgtatccata accagcgatg acgatgtaat
     4201 aagtggttcc ggcaggcatc gggatgtttt cgattttgga gttgagattg ccgcactcgg
     4261 ggctgtcgtc gttccaggcg atcgggtagc cgtcgcagtt tccgcggtag acccatacca
     4321 ccgtgtcatt ttcgtcgccc gagccgcaca gtgagatcgt aaccaactgg ctgccaacgt
     4381 gatgcgtcca tgactgactc agtaagctca aaatgcgcgg acatcgatgt tcatgagcag
     4441 caaccgttgg aattgcgcaa gcaagtcgaa tcggtgatgc tccggctccg ggcggccttc
     4501 gcgggcatcc tcgaatcagt tcccacacgc aacgggcgtc cgcacgaggt ggccgagctc
     4561 ttgaagctgg acaaacagct cgtgtggaag atatccaggc tggtcaagac gcgcgatccg
     4621 gtcatgatgg cgcgtcatct tccgggcccg acggctattc gcaggttttt ggatgcggcc
     4681 cagcttcatg tcgctccagg ggtaattgaa gcgatccatg cgccgctgga agacctcaac
     4741 gagctcattc gcgtgcatgc cggcgaccgc gccacgttcg agatgttgct ctcggagtgg
     4801 tgctctcgcg agccggacgc ggcggtgacg caccggcggg aggcctttcg tggaaatagc
     4861 tacatctggg gcgtgcaggc tcgaaccaag ctgacttcca tgttcctgca tccctcgtcc
     4921 gaggacgggg tctttgatat tgccatcgtc gcgggcgtcg tggatttgcg tcgcctcagg
     4981 ctgaacctgc cgtgggtgat cgcgcgttgg gcagtttcag acaacgatgg tgaggtgcgc
     5041 tttaatgcag accgcgagcc cctcgatccc gagtcgaatg atcccttgtt gcggcgtttt
     5101 tgcagcaaac cgctgccgca acttcgcatt gtcaatggcg caccgggcat gctggagtac
     5161 caactggcgc ccggcgcgat cggcaactcg ggcgcggtga cctgtattta cggcaacatc
     5221 gtgcgccggg tcgcctcgtg ctaccgcgac gaacacaacg agttcaacga ggtcatggcg
     5281 gccatgtcga ctccctgcga ggtgctcgtg ctcgaccaga tcgtccacga gagcctgttc
     5341 ggccggatta agccggacct gctcgtgtac agtggactga acgccagcga acagtacccg
     5401 cgcggcgtca cccgtgtgca gctcccggtc gccgagcagg tcgagtacct gggcaagggg
     5461 cctgcggtgt tgccgacgcc ccatgtcccg cggtatcgcg agctggccga atacgtcttt
     5521 gaacggacgg gctgggatgg cagccgtttc gacgtctacc gggcggagat gcgctatccc
     5581 ccgattccat caactgtcgt catgcaacac gccctacggg agcgccctgc ggggcgatag
     5641 cgtcgtctca tagtgcattt gaaagcttgc gcggcaggag ttgatctttc ggtccaccgc
     5701 ggtttccaag ccaagctggc tgccgattcg taagtctttg cgggaactgg cgtagaaaac
     5761 aagaacgccg ccgaggtctc tcggcggcgt tcggtcaaaa ccagcgggcc ggtcacgttt
     5821 gtaaccggcc ctgaagtagc gggggtagga ctcgaaccta cgacctccgg gttatgagcc
     5881 cgacgagcta c
//
LOCUS       DAOSYS010000220         5882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11410_length_5882_cov_5.182598, whole genome shotgun sequence.
ACCESSION   DAOSYS010000220 DAOSYS010000000
VERSION     DAOSYS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11410_length_5882_cov_5.182598"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLU99_12380"
     CDS             complement(<1..355)
                     /locus_tag="PLU99_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOO17890.1"
                     /translation="MTTQFEIGVIGAGNAAEGIVHGVLRNSVLLEDRIIASDPNPERR
                     RLFEQRFHIATTDDNCHLVRESRILLLAVKPQQYREVVREFGNLVREDHLVVSIMAGV
                     STASIENSLPHIKARV"
     gene            complement(536..1783)
                     /locus_tag="PLU99_12385"
     CDS             complement(536..1783)
                     /locus_tag="PLU99_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17891.1"
                     /translation="MCSGKPALTIAAYLWLIMMAASARAEQSVQQGRVLSHTSPIGGS
                     HVTLFLAGDEQGTGESVLGSDITNRHGFFRIPYTPPADPNAVLYLIADGPSAAVRFAT
                     VLGAPGYFENVTINERTTVATAYAMAQFVVDGRIGGRSPGLQNAAGTFRDLVDPVTGR
                     IAAVLGSPPNGLQTSTMRTFNSLANMLVGCVRSPNYATAFFRLATPPGGNAPDNALQA
                     AVNIAHYSWWNNSLLFYASLIYSPYRPVLWSAPETWALAIRYVGSVPDVGTVPEFGGP
                     GAVAFDPDLPQGVVADDAWVFDIDTGALADSAGGGMSIPWGIAVDGNDDVWVADFGGQ
                     RLTQFDGATGQPVAPEGYPSDALERSIGIAIDSAGNVWLCNTGPMTEMDSAQTNPSGD
                     GLVVFIGLAAPVKTPLPGPPQQP"
     gene            complement(2120..3598)
                     /locus_tag="PLU99_12390"
     CDS             complement(2120..3598)
                     /locus_tag="PLU99_12390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOO17892.1"
                     /translation="MDQGSLESGRGSGGLENPADFEASLGASIKPADPSEETVILPAP
                     PGSAEGIAAGQPAWAGKTLGHFRLLRLIGEGTMGMVIQALDIHLQRLVALKVLRKRIA
                     GLDEAKRVEQFLREARGAALIEHPNVVRIYEINQHAGWWYIAMEMVDGENMKQVVSAI
                     GRLPPDRACPLIADAATALAVAHELGIIHRDIKPTNLMITRGGRCKLTDFGLVRLNDP
                     NDPFDFTERSVGTPSFLAPELIQRHGITPAIDVYSLGATLYFALTGQPPYVGKTCKEV
                     LKQHVSAPVPDVREKAPDCPESLALLVQRAMAKDPATRPTAADLAASLHAESIAWRTD
                     GSGTSTLLVHGSGLLPDATGAIQQHAGPRRTWPSGRFYVIGVAVAAAIAVTAWYMASA
                     PPPEDHRPLAKLVERFPGAPETYGLLPAGQGCATAPVPAEAPEFSWVGKVDTAGLRYV
                     ASKRGRWYYAIDDPAARLIREEDFVGYATAQEAEAAGKSPAP"
     gene            complement(3612..4787)
                     /locus_tag="PLU99_12395"
     CDS             complement(3612..4787)
                     /locus_tag="PLU99_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17893.1"
                     /translation="MRAILELNRATFTCGRKSTRLFVLATAAMASIAWAPALARSPQP
                     RREAADIRFGVIEPPVVAQRDITIRAATSNQQREPVPLECSGLAWVDGSLLLVSDRHG
                     HVLFTCPVDLAKMTIGEPRPHVLIPNEQELLDDAECVALCPRGSDAWIGYALCSLSND
                     HRAVPLPQRRHMLRFVYSQTRGFTAERPVVLDAGTIRDALDEHFQAVGVEPYETFYAE
                     ADKNTYRWGNVEGLAFTPDGSRFLCALRNPLYHGQALLCAIEGLEEAFHLEDPARIRV
                     TDVFTLDLGQRGASDLCWDPVTKGYLIAAGKSNGPKRGQDEPFPPNTLDCALFWWSGR
                     KSEQPVLFARTPDMKVEAVCRLGESRYIAICSDEGDVSEERANRPQSVITIMDFSGW"
     gene            complement(5166..>5882)
                     /locus_tag="PLU99_12400"
     CDS             complement(5166..>5882)
                     /locus_tag="PLU99_12400"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOO17894.1"
                     /translation="ENLPSDFPHVVAVSSGGRLATLAEARGYTLIRIPLQYEPPGFQP
                     RSAVGYTATYLARVFHAAEALADSRPDLSAVPAFLRRHSTHEEARLTALWLADRIPVI
                     YTDEQHLLGIARPAKIKFNENAKRPAFFNAFPELNHNEMIGFRTKLARFAVLYFDDPA
                     SHPRIRRRFEVMRTTFAARGMDHVDFREWRIPGATRAEKVFAALEFADWCSYYRALLD
                     GLDPTPVDLVEEFKALLTAS"
BASE COUNT         1042 a         1812 c         1978 g         1050 t
ORIGIN      
        1 cgacgcgggc tttgatgtgc gggaggctgt tttcgatgct ggccgtcgaa acgccggcca
       61 tgatcgagac gaccaggtga tcctcgcgca ccaggttgcc gaactcgcgg acgacttcgc
      121 ggtactgctg gggcttgacg gccaggagca ggatgcggct ttcgcgcacg agatggcagt
      181 tgtcgtcggt ggtcgctatg tggaaacgct gctcgaacag gcggcggcgc tcggggttcg
      241 gatcggaggc gatgatgcga tcttccagca ggacgctgtt acggagaacg ccgtggacta
      301 tgccctcggc ggcgtttcct gcgccgatga cgccgatctc gaattgcgtg gtcacgctgg
      361 gctccttcgt tagacccagc ggcgcatgtt agccgatgac gggcgtcggc gaatatggcg
      421 ggcgacgttg ttggggagga gcgagatctt acctggggtc accgacggtc ggacgtcgcc
      481 actgcttgaa gctgcgcttc aagcagtggc acacgcgggc ggttttttgt cacgctcacg
      541 gctgttgcgg cggacctggc agcggggttt tgaccggggc cgcgaggccg atgaatacga
      601 ccaggccgtc gccgctgggg ttggtctgtg cggaatccat ctccgtcatc ggcccggtgt
      661 tgcatagcca tacattgccg gcggaatcaa tcgcgatgcc aatactccgt tccagagcat
      721 cgctgggata gccttcgggg gcgaccggct ggccggtggc gccgtcgaac tgcgtgaggc
      781 gctggccgcc gaagtcggcg acccagacgt cgtcattgcc gtcgaccgcg attccccagg
      841 ggatcgacat tcctccgcca gcggagtcgg ccagcgcgcc ggtgtctatg tcgaagaccc
      901 aggcgtcgtc ggcgacgacg ccttgcggca agtccgggtc gaaggcgacg gccccgggac
      961 cgccaaattc gggaacagtc ccgacgtcgg gaacactccc gacgtacctg atggccagcg
     1021 cccaagtctc cggcgcgctc cataagaccg gccggtacgg tgaatagatc aaagacgcat
     1081 agaacagtaa cgaattgttc caccacgagt agtgcgcgat gttaaccgcg gcctgaagcg
     1141 cattatcagg agcattgccg cccggcggtg tcgctagccg gaagaaagcc gtggcgtagt
     1201 tgggtgagcg gacgcagccg accagcatgt tggccaaaga attaaaggtg cgcatggtcg
     1261 aagtttgcag tccattgggc ggcgaaccga gtaccgctgc gatgcgcccc gtgaccgggt
     1321 cgaccaggtc gcggaacgtg ccggcggcgt tttgcagccc gggcgagcgg ccgccgattc
     1381 ggccatcaac gacaaattgc gccatggcgt aggcggtggc tacggtcgtt cgttcgttaa
     1441 tcgtcacgtt ctcgaagtac ccgggcgctc ccaggaccgt ggcgaaacga acggcagcgc
     1501 tcggcccgtc ggcgataagg tagagaaccg cgtttggatc ggccggcggc gtgtatggaa
     1561 tacggaagaa tccgtgccgg ttcgtgatgt ccgatcccag gaccgactcg cccgtaccct
     1621 gctcatctcc ggcgagaaaa agcgtgacgt gggacccgcc gatcggcgaa gtgtggctca
     1681 acacgcgccc ttgttgcacg gactgctctg cgcgtgcgga ggcagccatc attatcagcc
     1741 atagatacgc agctatcgtc agtgccggtt tcccgctaca catttcgggc tccttcactc
     1801 cggcaatcga ggcaagccga gcctgcactg cactttgagt gagtggtttc gcttactcga
     1861 tcgctttttc caagggacct gcgccctcgc gcacgacggg ctgcgttcac cggtcattct
     1921 agcgcggcag tgtgataaga agacgctcag gggggctcgc tccgttacga gcgcaccata
     1981 taagtcggct gtgtatggat ttgcgccgag ccgcattttt cgacgcggat tcggaattgg
     2041 cccaccagaa tgtggccgac atcagagata caaaaggtcc gggatatgag gctggcttat
     2101 tcgatccgac gcacagaacc tacggagcag ggctttttcc ggctgcttcc gcctcttggg
     2161 cggtggcgta gccgacgaag tcctcctctc gaatgagccg ggccgccgga tcgtcgattg
     2221 cgtagtacca ccggccacgc ttgctggcca cgtagcgcag cccggccgtg tccaccttgc
     2281 cgacccaact gaactcggga gcttcggccg gcacgggcgc ggtggcacag ccctgcccgg
     2341 caggcaaaag gccataagtt tcgggcgcgc cggggaaccg ctctaccagc ttggcgagcg
     2401 ggcggtggtc ctcaggcgga ggtgcactgg ccatgtacca cgctgtgacg gcgatggccg
     2461 cagccaccgc cacgccgatc acatagaagc gccccgacgg ccatgtccgc cgcgggccgg
     2521 cgtgttgctg gatcgcgccg gtcgcgtcag ggaggaggcc gctgccgtga acgagcaagg
     2581 tagaagtccc gctgccgtcg gtgcgccagg caatcgactc ggcatgtaga gaggcggcca
     2641 ggtcagcagc cgtcgggcgg gtggcggggt ccttggccat ggcgcgctgc accagcagag
     2701 ccagcgattc cgggcagtca ggagctttct ctcgcacatc gggaaccggt gcagagacgt
     2761 gctgcttgag gacttccttg caggttttgc cgacataggg cggttgaccg gtcagggcaa
     2821 agtacagcgt tgcgcccagg ctgtagacat cgatggccgg cgtgatgcca tggcgctgaa
     2881 tcagttccgg cgccagaaag ctcggggtcc ccacggagcg ctcggtgaag tcaaacggat
     2941 cattgggatc attcaagcgc accagcccaa aatcggtcag tttgcagcgc ccgccgcggg
     3001 taatcatcag gttggtaggc tttatgtcgc ggtgaatgat gcccagctca tgcgcgaccg
     3061 ccagcgcagt ggccgcgtcg gcgatcagcg ggcaagcccg gtcgggcggt aagcgcccga
     3121 tggccgagac cacttgcttc atgttctcgc cgtcgaccat ttccatggcg atgtaccacc
     3181 agccggcatg ctggttgatc tcgtagatgc gcaccacatt gggatgttcg atcagggcgg
     3241 ccccgcgtgc ttcacgcaga aactgctcga cgcgtttggc ctcgtccagg ccggcgatgc
     3301 gtttgcgcag aaccttgagc gccaccaggc gctgcaggtg gatatccagg gcttggataa
     3361 ccatccccat cgtgccctcg ccgatcaaac gcagcagccg gaaatggccg agcgtcttgc
     3421 cggcccaagc cggctggccc gccgcgatgc cctccgcaga cccgggcggc gcagggagaa
     3481 tgaccgtttc ttccgaggga tctgcgggct taatgctggc gccgaggctc gcctcgaagt
     3541 cggccggatt ttccagcccg ccgctacctc tcccagattc cagcgaacct tgatccaagg
     3601 gtcgcgctct cctaccaacc cgaaaaatcc atgattgtta tgacgctctg aggacggttg
     3661 gcccgctctt cactgacgtc gccctcgtcc gagcagatcg caatatagcg gctctctccc
     3721 aatcggcaga cggcctcgac cttcatgtca ggagttcggg cgaacagcac cggctgctcc
     3781 gacttccgcc cgctccacca gaacagggcg cagtccagcg tgttaggcgg gaaaggctcg
     3841 tcttggccgc gcttcgggcc gttgctcttg ccggccgcaa tcaggtagcc cttcgtcacc
     3901 gggtcccagc agaggtcgct ggcgccgcgc tgacccaggt ccaaagtgaa aacatcggtg
     3961 acgcgtatcc gggcggggtc ttcaaggtgg aaggcctcct ccaggccttc gatcgcacac
     4021 agcagcgctt ggccgtggta cagcgggttg cgcagggcac ataggaaccg cgagccgtcg
     4081 ggagtaaagg cgaggccttc cacgttgccc cagcggtagg tgttcttgtc ggcctcagcg
     4141 tagaaggtct cgtagggttc gacgcccacg gcctggaagt gctcgtcgag ggcgtcgcgg
     4201 atcgttcccg catcgagcac gacgggccgt tcggccgtga agccgcgggt ctggctgtag
     4261 acgaagcgca gcatgtggcg ccgctgcggc agcggtacgg cgcggtggtc gttgctgaga
     4321 gaacagaggg cgtagccgat ccacgcgtcg ctaccccgcg gacatagcgc gacgcactcg
     4381 gcatcgtcca acagctcctg ctcattagga atgagcacgt gcggacgcgg ttcaccgatg
     4441 gtcatcttcg ccaggtcgac ggggcaagtg aacaggacgt gcccatgccg atcgcttacc
     4501 agcaacagcg agccatcaac ccacgccaag ccgctgcatt cgagcggaac gggctcgcgc
     4561 tgctggttgg aggtggccgc gcggatcgta atgtcgcgct gggcaacgac cggcggttct
     4621 atcacgccga accggatgtc ggccgcctcg cggcgcggct ggggagaccg ggccagcgcc
     4681 ggcgcccatg cgatgctcgc catcgctgcc gtcgcgagca caaacagccg ggttgacttg
     4741 cggccgcagg tgaacgtcgc acggttcagt tcaaggatcg cgcgcatcgg tcaacctctg
     4801 agttgcgggc agctcgcagc ctgacaaact ggccggatgt agtcaaaccc attttttgaa
     4861 gcgccgcggc cgagttcaaa ctcttagttt aatggctttg tgggggcgga gcaaactcat
     4921 ctgccaagcg ggggccaggg cgggctggcc ggcgggttgg tcttctgagg cagcgttggg
     4981 gtattggagc ccagccggca aacacgagat agcgggcccg gaagtccgct ggtgcggagt
     5041 gaacgcggga ggccgcgcag gtccggccgg ccagcagcgc gagtgcgggg cccatcgtgt
     5101 taatccgtac cgccgggcgc ttcttggccc gcggcgcaca tccagacggg ccctttctag
     5161 gtttcctacg acgctgtcag cagcgccttg aattcctcga caagatccac cggcgtaggg
     5221 tccaggccgt ccaacagcgc gcggtagtag gagcaccaat ccgcaaactc cagcgccgca
     5281 aagacctttt cggcccgggt agcgccgggt attcgccact cgcggaaatc cacgtggtcc
     5341 atcccccggg ccgcgaaggt ggttcgcatg acctcgaagc gccggcggat gcgcgggtgg
     5401 ctggcgggat catcgaaata cagcaccgcg aagcgtgcca gcttcgtcct aaacccgatc
     5461 atctcgttgt ggttcaactc gggaaaggcg ttgaagaacg ccggacgctt ggcgttctcg
     5521 ttgaacttga ttttcgccgg gcgggcgatg cccagcaggt gttgctcgtc cgtgtagatg
     5581 accggaatgc gatcggccag ccatagggcg gtcaggcgcg cttcctcatg cgtactgtgc
     5641 cgccgcagga aggcgggcac ggcgctcagg tccggccgtg aatccgcaag cgcctccgcg
     5701 gcgtggaaga cgcgcgccag gtaggtagcc gtgtaaccga cggcggagcg cggctgaaat
     5761 ccgggcggct catactgcag cggaatacgt ataagcgtat agccgcgcgc ttcggccagg
     5821 gtggcgaggc ggccgccgct gcttacggcg acgacgtgag ggaagtcgct gggcaggttc
     5881 tc
//
LOCUS       DAOSYS010000221         5872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11426_length_5872_cov_5.922297, whole genome shotgun sequence.
ACCESSION   DAOSYS010000221 DAOSYS010000000
VERSION     DAOSYS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11426_length_5872_cov_5.922297"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLU99_12405"
     CDS             complement(<1..198)
                     /locus_tag="PLU99_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17895.1"
                     /translation="MPTPAFTNTNGRPLIGISMGDPLGVGPEVTVKALADPELRSQAR
                     FIIFGLHDVLAVAADAAEINPF"
     gene            complement(198..875)
                     /locus_tag="PLU99_12410"
     CDS             complement(198..875)
                     /locus_tag="PLU99_12410"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOO17896.1"
                     /translation="MFTGIIESLGTVVHLAHAPRNGTSDAAAQRLEIDGGGLFAELRP
                     GASVAVNGACLTLTAIRQAIGCFDVIPETWHNTALRLLRPGDAVNLERALRIGDRLDG
                     HFVQGHVDGVGSVVRVDRSGGQWKLWLSTPADLRPYIVRKGSVALDGVSLTVVDVTDE
                     GFSVALIPVTRERTLLGRRKPGDLVNIETDVLARFVLSRLEALRGSEPSKTDTITLEA
                     LRDRGFV"
     gene            complement(1102..1482)
                     /locus_tag="PLU99_12415"
     CDS             complement(1102..1482)
                     /locus_tag="PLU99_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17897.1"
                     /translation="MLKTKIAGFVLAGGVLLSLGGCPGVDPLLTGLAKIASGNIQTLT
                     VAEVQNVAIWAEQQAGVQQMPLTEEEAQAVLWFLEDNNINTIEDVSYLVHNPQEVIIS
                     EDVAQVVGKDRANQVLAFLGQSGV"
     gene            complement(1496..>1684)
                     /locus_tag="PLU99_12420"
     CDS             complement(1496..>1684)
                     /locus_tag="PLU99_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17898.1"
                     /translation="TDAQAQDIANFFDSNGVETPADLATVLGDPVVAAGLPADLDSDT
                     LIGLFVDFDPELLLPELP"
     assembly_gap    1685..1784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1986..3053
                     /locus_tag="PLU99_12425"
     CDS             1986..3053
                     /locus_tag="PLU99_12425"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOO17899.1"
                     /translation="MRVAIVGFPYSGKTAVYRAVTGLSREQIRPAEENLAAVKIPEPR
                     LDFLADLFKPKRKTEGTMEFVDLPGSAEGDREQAGLSKHLPTLRESEALLIVVRAFDS
                     ASVLPHQGRVDPQRDLQQLRDEMLLADLAICAARIDKLEKAVTKPTKDVEQLKHELAL
                     LTRCRDALENEKPLLNVVQPGEEEKMLRAFGFLTQKPEVLIINVNEEQAASPPPFRDA
                     YAAATFAICAPLEADLISMDPADRAGFMQDYGIQALARDRIVRACFDALGMISFLTGG
                     PGGEEVRAWPIPKGATAVEAAGKIHTDIARGFIRAETIAYEDLRAAGSLREAKAANKI
                     RQEHKNYVVQDGDVITFKFSA"
     gene            3082..5133
                     /locus_tag="PLU99_12430"
     CDS             3082..5133
                     /locus_tag="PLU99_12430"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOO17900.1"
                     /translation="MYATLTSLFLLLPAVAPPPAQTAAAADAPAVTQADLLNRLIDVD
                     RLYSPPAGERTGMFSSGPKPPAFLRTQADGWSVMAELSGPGALTRIWSPNPQGRIRIE
                     LDETNSFELNFADLFSGRTPPFETPLCYVADDGGHVCYFPIGFSKGCRILARDCESLY
                     EISYVLFPPGTNVQSFARELDEPALAALDAVITAWDASAAGKRPASDVRTYVLSNRQE
                     LRAGEKLTAESFDGGGIIRSLQVSIAGRLPEARYLLHQFILRIYFDNQEQPAVEAPLI
                     DFFGSGLTLTRFCSLLGGTDLWTDIPGEATPENLYRLTQESRFMYCYFPMPFADVARI
                     EIEAPPNVHKARPVSLMLYALIQRDKPPAGALQFHARFRGEDPCEKEDYSVLEARGPG
                     RIVGCVLNAASPQGGWGQAGGPRIWIDGDKAPRYAGACIQALIGRPPDAGRPVREPAN
                     PLHGVTAAGRFGKCSGYRWFLSDSIDFERSARFAIDNSEAAPRETSYSTLVYWYADPQ
                     AGQPGGRRLFVPLTLDNLTPGPLRIAGAVEVEDHVVGGVDRVLKEQYADGNELSGQLA
                     AIIPDTGPVQINIPSETERVVHLTLRMHQLRERYFDKVEIIDPDGRPLTTIKYDRHSG
                     GLYPVGRVRLKAGDNLFTVICSKRAVLDCWILQDAPDAEADEAVEANEPDQAAKADQA
                     G"
     gene            5304..>5872
                     /gene="guaA"
                     /locus_tag="PLU99_12435"
     CDS             5304..>5872
                     /gene="guaA"
                     /locus_tag="PLU99_12435"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOO17901.1"
                     /translation="MPHHETIAILDFGSQYSQLIARRVRENHVYSELLAPDTPAHSLR
                     KMGVRGIILSGGPGSVYEAGAPRCEPAIFELGVPVLGICYGMQIACQILGGEVRAAQA
                     REYGRVRLNITRPEGVLAHLPQEISAWMSHADVVMRVSDSFESIAATDSCPIAAVRHR
                     ERPVYGVQFHPEVTHTPQGSQIIRNFLYDV"
BASE COUNT         1100 a         1919 c         1764 g          989 t
ORIGIN      
        1 gaaggggttg atttccgccg catcagcggc gaccgccagc acatcgtgca ggccgaagat
       61 gatgaagcgc gcttgtgaac gcaactccgg gtcggccaac gctttgaccg tgacctcggg
      121 gccgacgccg agcggatcgc ccatgcttat gccgatgagc gggcggccat tcgtgttggt
      181 aaacgccggg gtaggcatca aacaaaaccg cggtcgcgca gcgcctccaa cgtgatagtg
      241 tcggttttgg agggctcgct cccccgtagc gcctctagcc ggctcagaac gaaccgcgcc
      301 aggacgtccg tctctatgtt tacgaggtcg ccgggcttgc ggcggccgag caacgtgcgc
      361 tcgcgcgtca cgggaatcag cgcgaccgag aagccttcgt cggtgacatc gaccacggtc
      421 aggctgacgc cgtccagggc gaccgagccc ttgcgaacga tgtacggccg caaatcggcc
      481 ggagtggaaa gccacagctt ccactggccg ccgctacgat ccacgcgcac gactgacccc
      541 acgccgtcga catggccctg cacgaagtgc ccgtcgaggc gatcgccgat gcgtagcgcg
      601 cgctcgagat tgacggcatc accgggacga agcaatcgca gagcggtgtt gtgccaggtt
      661 tccggaatta catcgaagca gccgatcgcc tggcggatcg ccgtcagcgt caggcaggcg
      721 ccgttgacgg ccacgctggc gccgggccgc agttcggcga acagcccgcc gccgtcaatc
      781 tcaagccgct gggccgccgc gtcggacgtg ccattgcgcg gcgcgtgcgc cagatgtacg
      841 acggtgccca agctttcgat gatcccggta aacaagcgta actctcgcgt gcggcggctt
      901 tcaaagtgcc gaggcccaca actactgata ttagaggcgg agtcgcgatt tcgggcaaga
      961 atcaggcgga gctgcgatca cgggccgggc ctcgacgcgc ctgagaagca catccggcgc
     1021 aaaaaagcag atggccggtc gcgcgagcgc ccggccaaat cgtgagggca aattcggctg
     1081 ctttcaggat gtcgggctga atcagacccc cgactgcccg aggaaggcca gcacctggtt
     1141 ggcgcggtcc ttgccgacca cttgggccac gtcttcggag ataatcacct cttgcgggtt
     1201 gtggaccagg taactcacgt cttcgatcgt gttgatgttg ttgtcctcca ggaaccagag
     1261 cacggcttgc gcttcttcct cggtcagagg catttgctgt acgcccgctt gctgttcggc
     1321 ccagattgcc acgttctgca cttcggcgac ggtcaaggtc tggatgtttc cgctcgcgat
     1381 cttggcgagc cccgtgagca gcgggtccac gcccggacat ccgccaaggc ttaatagcac
     1441 gcctcctgcg agtacgaatc ctgcgatttt cgttttcagc atgccgatct ccaggtcagg
     1501 gcaattccgg cagcagcagc tcgggatcga aatcgacgaa cagcccgatc agtgtgtcgg
     1561 aatccaggtc tgccggcaga cccgctgcta cgactggatc gccgaggacc gtagccaggt
     1621 cagccggggt ttccaccccg ttactatcga aaaaattggc tatgtcctgt gcctgggcgt
     1681 cggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncacaag cggtcagaag
     1801 gcctgctacc aacagcaaaa cggtcaagac actcgcacgc atgatgcctc caatcatttc
     1861 tcagccaacc tcgcctgacc aagagcctac gcgcataccg aatgtccgta tagtatatcg
     1921 tctaatggcg gtacgctccg ccgccaatgg cgccaggcgg ccgcccgata agccgggaga
     1981 ccagcatgcg cgtcgcaatc gtcggctttc cgtattcagg caaaaccgcg gtatatcggg
     2041 ccgtcaccgg actatcccgc gaacagattc gcccggccga agagaatctg gccgccgtca
     2101 aaatccccga gccgcgactg gacttcctgg ccgacctctt caagccgaag cgcaagaccg
     2161 aaggcacgat ggagttcgtc gacttgccgg gcagcgccga gggcgatcgg gaacaggccg
     2221 gcctgagcaa gcatctgcct accttgcgcg aatcagaagc gttgctgata gtggtccggg
     2281 cgttcgattc ggcctcggtc ctgccgcacc agggacgggt tgatccgcaa cgcgacttgc
     2341 agcagcttcg ggacgagatg ctgctggcgg acctggcgat ttgtgcggcg cgaatcgaca
     2401 agcttgaaaa agcggtcacc aagccgacca aggacgtgga gcaactcaag cacgaactgg
     2461 ccctccttac ccgctgccgg gacgcgctgg aaaacgaaaa gcccctgctg aacgtcgtgc
     2521 agcccggcga ggaggagaag atgttgcggg ctttcgggtt cctcacgcaa aaacccgaag
     2581 tcctgatcat caacgtcaac gaggagcagg ccgcctcacc gcccccgttc cgcgacgcct
     2641 acgcggccgc cacgttcgcc atctgcgctc cgctggaggc cgacctgatc agcatggacc
     2701 ccgcggaccg ggccggcttc atgcaggact atggtattca ggccctggcc cgcgaccgca
     2761 tcgtgcgggc ctgcttcgac gcgctcggca tgatcagttt cctgaccggc ggcccgggcg
     2821 gcgaggaggt ccgcgcctgg ccgatcccca aaggcgctac cgccgtggag gccgccggca
     2881 agattcacac cgacatcgcg cgcggcttca tccgggccga gacaatcgcc tacgaagact
     2941 tgcgggccgc cggctcgctg cgcgaggcca aggcggccaa caagattcgc caggagcaca
     3001 agaactatgt cgtgcaggac ggggacgtga taaccttcaa attcagcgcc tagcgggcgg
     3061 gccgcgaagc cggagttctt aatgtacgcc accttgacca gtcttttcct gctcctgccg
     3121 gctgttgcgc cgccgccggc gcagaccgcc gccgccgcag atgcgccggc tgtcacccag
     3181 gccgacttgc tgaaccggct aatcgacgta gaccggctgt attcaccgcc ggccggagag
     3241 cgcaccggaa tgttttcgag cggcccgaag ccgccggctt tcctgcgcac ccaggccgac
     3301 ggttggagcg taatggccga actcagcggc cccggagcgc tgacgcgcat ctggtctccc
     3361 aatccgcagg gccgcattcg gattgaactg gacgagacaa acagctttga gctgaacttc
     3421 gcggatctct tcagcgggcg gacgccgccg tttgagacgc cgctatgcta tgtcgcggat
     3481 gacggcgggc atgtctgcta cttcccgatt ggtttttcga agggctgccg catactggcg
     3541 cgcgactgtg agtctctata cgagatcagc tacgtcctgt tcccgcccgg aacgaacgtg
     3601 cagtcgtttg cccgcgaact ggacgagccg gcgctcgccg cgctcgatgc cgtgatcacc
     3661 gcttgggacg cctccgccgc cggcaaacgc ccggcgagcg acgttcgcac ctatgtcctc
     3721 agcaatcgcc aggaactgcg cgcgggcgaa aaactcacgg ccgagagctt cgatggcggc
     3781 ggaatcatcc gttccctgca ggtatccatc gccggccgcc tccccgaagc ccgctacctg
     3841 ctgcaccagt tcatactgcg catctacttt gacaaccagg agcagccggc cgtcgaggcg
     3901 ccgctgatag atttcttcgg ctcgggcctg acgctgacgc gcttctgtag cttgctgggc
     3961 ggaaccgacc tgtggactga cataccgggc gaggccacac ccgaaaatct ctaccggctc
     4021 acgcaggaaa gccgtttcat gtactgctat ttcccgatgc cgtttgcgga tgtggcccgg
     4081 atcgagatcg aagccccgcc caacgttcac aaggcccggc cggtctcgct catgctgtac
     4141 gcgttgatcc agcgcgacaa gccgccggcc ggcgcgctgc aattccatgc tcgctttcgc
     4201 ggggaggacc cgtgcgagaa agaagattat tcggtgttgg aggcgcgcgg gccggggcgc
     4261 atcgtgggat gtgtgctgaa cgccgcatcg ccgcaggggg gatggggcca ggccggtggc
     4321 ccgcgaatct ggattgacgg cgacaaagcg ccgcgctacg ccggcgcgtg catccaggct
     4381 ctgatcggcc gcccgccgga cgctggccgc ccggtccggg agccggcgaa tccgctgcac
     4441 ggggtcacgg cggccggccg attcggcaag tgctccggct accgttggtt cctttccgat
     4501 tcgattgact tcgaacggag cgcgcggttc gccattgaca attcggaagc ggccccgcgt
     4561 gagacgtctt acagcacgct ggtctactgg tatgcggatc cgcaggccgg ccagcccggc
     4621 ggaagacgac tttttgtgcc gctgacgctg gataatctga cgccggggcc gctgcgcatc
     4681 gcgggtgcgg ttgaggtgga ggaccacgtc gtcggcggtg tggatcgcgt gctcaaggag
     4741 caatacgccg acggcaacga gctttccggc cagctcgccg cgatcatccc cgacaccggg
     4801 ccggtgcaaa tcaacatccc ctcggaaacc gagcgcgtgg tgcacctcac attgcgaatg
     4861 caccagctcc gcgagcgcta cttcgacaag gtcgagatca tagaccccga cggccgccca
     4921 ctgacgacga ttaagtacga tcgacacagc ggcggcctgt acccggtagg ccgcgtccgc
     4981 ctgaaagccg gcgacaacct gttcacggta atctgctcca agcgcgccgt actcgattgc
     5041 tggatcctac aagacgcccc ggacgccgaa gccgacgaag ccgtcgaagc aaacgaacca
     5101 gaccaagccg ccaaagcaga ccaggcaggt taagcaacca aagcagccga aggtgataag
     5161 ttgataaagt gatagagtga taaagaacgt cccgcggccc ctttatcacc ctatcacttt
     5221 atcactttat caccgctccc acctttaaac ctttcgcctc tcgccgattt gaccaccgcc
     5281 cagcgatgtg ataatcctgg tatatgcctc accacgaaac aatcgcgatc ctcgatttcg
     5341 gcagccaata ctcccagctc atcgcgcgcc gcgtccgcga aaaccacgtt tacagcgaat
     5401 tgttagcccc agacacgccc gcccactccc tgcgcaaaat gggcgtgcgc ggcatcatcc
     5461 tgtcgggcgg ccccggcagc gtctacgagg ccggcgcccc acgctgtgaa cccgccatct
     5521 tcgaactcgg cgtccccgtc ctgggcatct gctacggcat gcagatcgcc tgccaaatcc
     5581 tcggcggcga ggtgcgggcc gcccaagccc gcgaatacgg ccgcgtgcgc ctcaacatca
     5641 cgcgccccga aggcgtgctg gcccatctgc cccaggaaat ctcggcctgg atgagccatg
     5701 ccgacgtggt aatgcgggtc tccgactcgt tcgaaagcat agccgccacg gatagctgcc
     5761 ccatcgccgc cgtgcgccac cgcgagcgcc cggtctacgg cgtccagttt catccggagg
     5821 tgacgcatac cccccagggc tcgcagatca tccgcaactt cctctacgac gt
//
LOCUS       DAOSYS010000222         5824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11537_length_5824_cov_5.406483, whole genome shotgun sequence.
ACCESSION   DAOSYS010000222 DAOSYS010000000
VERSION     DAOSYS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11537_length_5824_cov_5.406483"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PLU99_12440"
     CDS             <1..1261
                     /locus_tag="PLU99_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17902.1"
                     /translation="ARAVRGVKGWTAISVLMGCLFAAVYFFYGQQLGRLGVFDRIDML
                     FDADPKFHQSGWGTEIHTSLHPLLPAGWYLSCTLASRAVAAEWAPLAVSAGFGGLCVT
                     LAALYFKKVTGSRLLGLAGAFLLGCTTAHLSFAAMPESYIVAAATLISLQLLVAHRQS
                     LRLRFRHAVLVGVLATGVTITNAAAALVCYCCWRRTRRAAYVVRWAAYSLLIGSALLA
                     AQSLILPQACGLEPSEYVYQQRFLHTQTPLGQRLAGLVNASLVQNVVGWIPAPAVIYG
                     RPALRAGLQYDRLGQATVWLWAVSSLAAAWMVWRRRTWGSATFVAALLCLLVAASFHS
                     QYGYDNLFLYSCCFTFYVLALPAHGLAGSRSAPVALIVVVICVAMAVNNARFCKRVPA
                     MLGELERARGWVSPEVTDPGKAVHPES"
     gene            1485..2441
                     /locus_tag="PLU99_12445"
     CDS             1485..2441
                     /locus_tag="PLU99_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOO17903.1"
                     /translation="MTELYAPIRSELAESQAIFRDELISDQGFIADLCGHVAQFHGKL
                     LRPALLLLTGHACGTLRPAHSVLAAVVEMVHIATLVHDDVLDEADVRRRAATVNRLWG
                     NERAVLMGDFLISHAFHLCSSLDSQFAARLIGATTNTICEGEMMQVSNRDNLELTEGE
                     YLDIIRRKTAALLGTCCLLGAKYAGADESRTEQMRRCGESLGLAFQIVDDVLDLVGDE
                     AEVGKSLGRDAEMGKMTLPLIYCLQHGTPEERTELCALLRTGEPRRRVAEIARAMGGI
                     EYAQGVAARYVAEARNALAGLPPSGARDSLNAMADFVLARHE"
     gene            complement(2524..3234)
                     /locus_tag="PLU99_12450"
     CDS             complement(2524..3234)
                     /locus_tag="PLU99_12450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOO17904.1"
                     /translation="MAPSTGMAPNLAPGAAPGVDDAELPPLCDRELSPAEAAAELARL
                     NEHFVRHCRKCELHANRTQTVFGVGRPRPELVFVGEGPGADEDRQGQPFVGRAGQLLT
                     RMIAAMTLTREQVYICNVVKCRPPDNRTPTEPEMEVCSPYLWRQLAILRPRVIVALGR
                     PATQTLLVTNVPIGRLRGEFQDFPPPPLAHLGLPRCKLMPTFHPAYLLRSPGEKAKAW
                     EDLKQVMRELGIPIPQAK"
     gene            3610..5361
                     /locus_tag="PLU99_12455"
     CDS             3610..5361
                     /locus_tag="PLU99_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOO17905.1"
                     /translation="MALILAGALIATAWATEPAPPAADSPAAPSSVLLSPTLGRPVFV
                     EPGATFEALAQVAEAAGPVTVKLISTSAPAVTRSLPLPPDLESSIADGRPLLLRVPAD
                     LPERTYDLEITLGASRLVGRHCVAVKRLGPQIRIVHLSNMNIGDLAAPDFDRRLVDDV
                     NLVAPDLIIASGDFVDRTAENPEALWERLPEFFSQFDAPAFIACGDHDDLQQYSKHVA
                     VNPIGSVELGFLRGFAIYDRPSRPISDDPGQQQWLERALTAPGDERLKFVVAHDDRPN
                     LLLQWRKRGVLPKMISSTRLGLWISGSHRDWDGVEYADIVRQAAPLMYVRTHQSSTAT
                     RDGATGVSHFRVLDVTNERVAAPGWNSSDVAPPSLPTGLLTCTSECPAENVVAVTVAN
                     NHAFRINGLQARVLVAGVPEARPWCLGAELVSSHMIDGVWDCRLRFDVPDKGTARAVV
                     GSGSTPLPPRFTVRFDMPTVLRLTPQQTPEGLEYASAANVNALIYITNSGAVDADFTP
                     LLRLDGDTLSYKVIEEPGPPALAYRLHLPPEGSVTLQPDLSALRTTPGRRELQVYLTG
                     LPALAPACQPLTVITAP"
     gene            5558..5631
                     /locus_tag="PLU99_12460"
     tRNA            5558..5631
                     /locus_tag="PLU99_12460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5592..5594,aa:Ile,seq:cat)
BASE COUNT          925 a         1881 c         1925 g         1093 t
ORIGIN      
        1 ggcgcgggcc gtccgtggcg tcaaaggctg gaccgccatc tcggtgctga tgggttgctt
       61 gttcgcagcc gtctatttct tttatgggca acagttgggc cggctgggcg tgttcgaccg
      121 gatcgatatg ctgttcgacg ccgatccgaa gttccaccag tcggggtggg gtaccgaaat
      181 ccacacgagc ctgcatccgc tgctgcccgc aggctggtat ctgtcgtgca cgctggcctc
      241 gcgcgccgtg gccgccgaat gggcgcccct ggccgtcagc gcggggttcg ggggtttatg
      301 cgtgacgctg gcagcactgt acttcaaaaa agtgaccggc tcgcgcctgc tggggctggc
      361 gggggcgttt ttgctgggct gcaccacggc gcacctaagt ttcgcggcca tgccggagtc
      421 gtacatcgtc gcggcggcaa cgctgatctc gctgcaactg ctggttgcgc accgccaatc
      481 gctgcgcctc aggttccggc acgccgtgct cgtgggcgtg ctcgcaacgg gggtgacgat
      541 caccaacgca gccgccgcgc tggtgtgcta ttgctgttgg cgcaggacgc gccgcgcagc
      601 gtacgtcgtg cgctgggcgg cttattcctt gctgatcggg tctgccctgc tggcagcgca
      661 gtcgctcatt ctgccgcagg cgtgcgggtt ggagccgagc gaatacgtgt atcagcagcg
      721 gttcctacac actcagaccc cgctcggcca gcgcttggcc ggcttggtaa acgcctcgct
      781 cgtacagaac gtggtagggt ggattccggc tccggccgtg atctatggcc ggccggcact
      841 gcgcgccggc ctgcaatacg accgtctagg acaggcgacc gtctggttat gggcggtgtc
      901 cagcctggcc gcggcgtgga tggtttggcg ccggcggacc tggggcagtg cgacatttgt
      961 ggcggcgctg ctctgtttgc tggtcgcagc cagcttccat agccagtacg gctacgacaa
     1021 tctgttcttg tactcgtgct gcttcacctt ttacgttttg gcgcttccgg cacacggcct
     1081 ggccggcagc cgctcggccc cggttgcact gatcgtagtc gttatctgtg tggccatggc
     1141 ggtgaataac gcgcgcttct gcaagcgcgt tccggctatg ctgggtgagc ttgagcgtgc
     1201 ccgcggttgg gtctccccag aggtaaccga tcctggcaag gccgttcatc ctgaatcgta
     1261 aaggacgggt ggcgggcggt ttcacgctca cccgcccatt gtagacccct tgagcccttg
     1321 atccgcacct aaccttgacg ctgcacaggc ggaccgcgcg ttctcaagat ttcaatggtt
     1381 ctgaaagttc agtgcagact ggacatgcct ctatcgcgcg gaaagactct gccgtgagtc
     1441 ttaaggagac aagggtgcag gcgccgacgc gcgcaacact tcgtttgacc gagctatacg
     1501 cgcccatcag gtccgagttg gccgaatcgc aggccatctt ccgcgatgag ctgatcagcg
     1561 atcaaggctt catcgccgac ctctgcgggc acgtcgccca gttccacggc aagctgttgc
     1621 ggccggccct gctgctgctg accggccacg cttgcggaac cttgcgcccg gcccacagcg
     1681 tgttggcggc cgtggtcgag atggtgcaca tcgcgaccct cgttcacgat gacgtgctcg
     1741 atgaggcgga cgttcgccgc cgggcggcca ccgtaaaccg gttgtgggga aacgagcggg
     1801 ccgtgctgat gggggatttt cttatcagcc acgcctttca cctgtgcagc agtctggatt
     1861 cgcagttcgc cgcccggctg atcggagcga cgacaaacac gatctgcgaa ggcgaaatga
     1921 tgcaggtcag caatcgcgac aatctggagc tcactgaggg ggaatacctg gacatcatcc
     1981 gccgcaagac ggcggccctc ctcggcacct gttgcctgct gggcgccaag tacgccgggg
     2041 ccgatgagag ccggacggaa cagatgcggc gctgcggcga gtcgctgggg ttggcgttcc
     2101 agatcgtgga tgacgtgttg gacctggtgg gcgatgaggc ggaggtgggc aagtccctgg
     2161 gccgcgacgc cgaaatgggc aagatgacgc tgccgctgat ttactgtctt cagcacggca
     2221 cgcccgaaga gcgcaccgaa ctatgcgcgc tgctccgcac cggtgagccg cggcggcgcg
     2281 tcgcggaaat cgcgcgtgcg atgggcggaa ttgagtacgc gcaaggggtc gcggcccgct
     2341 atgtggccga ggcgcgcaac gcgttggccg gcctgccgcc gtcgggggcc cgcgacagcc
     2401 tgaacgcgat ggccgatttc gtgctggcgc ggcacgagta ggagaagggc aacgaggcac
     2461 cctggccggt ccgccgctcg cagcccgttg ctgaaattca gatgccgccc cggggctgtc
     2521 cgctcatttc gcttgcggaa tcggaatgcc aagctcacgc atgacctgct taaggtcctc
     2581 ccaggctttt gccttttcgc cagggctgcg cagcaggtac gccggatgga acgtcggcat
     2641 tagcttgcac cgcggcagcc ccaggtgcgc cagcggcggc ggtggaaaat cctggaattc
     2701 gccgcgcagc cggccgatcg ggacgttcgt gaccagtagc gtctgtgtcg ccggcctgcc
     2761 caaggcgacg atgacccgcg gccgaaggat tgcgagctgc cgccacaggt aaggcgaaca
     2821 gacttccatc tccggttcag tgggcgtgcg gttgtccggc gggcggcatt tcacaacgtt
     2881 gcaaatgtag acctgctcgc gagttaaggt catggcggcg atcatgcgcg tcaggagctg
     2941 gccggcccgc ccgacaaacg gctggccctg gcggtcttcg tccgctccgg ggccctcacc
     3001 gacgaagacc aactccggac gcgggcgccc gacgccgaat acggtttgcg tccgattcgc
     3061 gtgcagctcg cattttcggc aatggcggac gaaatgctcg ttcaggcgcg ccagctcggc
     3121 cgccgcctcg gctggtgaca attcccggtc gcacagcggc ggcaattcgg cgtcgtccac
     3181 gccgggtgcc gcgccaggtg ccaagttggg cgccatgcca gtactgggtg ccatgcccct
     3241 gctgggtgcc atgcccgaac tgggggccat gccggtgctg ggtgccatgc ccaagcctcg
     3301 cgcagcgggc gcgggcatgc ccctgccgac aggcggacga ccggttgaag gcggccctgc
     3361 gagaaaattc gagctggacg cacgcgcccc cagcatgccg gcgcggcctt tcgcccaaac
     3421 gtcgccgacc tccgcgtggg ctatcccgag ccgccgttcg ctccgcagcc actcgcccaa
     3481 aacaactttt ttctcggcat cttctaaatt gctcacgatt cgggagaata ggggcacggg
     3541 ccgcaagcga caaggctgaa catgaatcag aggttgccag gcagacggca caagcaaacc
     3601 atgccgggaa tggcgctaat tctcgccgga gcgctcatcg ccacggcctg ggccacggag
     3661 cccgcgcctc cggccgccga ttcgccggcg gcgccctcat ccgtgttgct ttcacccacg
     3721 ctcgggcggc cggtttttgt tgagccggga gcgaccttcg aggctttggc gcaagtggct
     3781 gaggcggccg ggcccgtcac ggtgaagctg atctcgacca gcgcgccggc ggtgacccgc
     3841 tcgctgccat taccgcctga tctggagagc agcatcgctg acggccgccc gctgctgctg
     3901 cgcgtccctg cggatctgcc cgagcgaact tacgacctgg aaatcacgct cggcgcgtcg
     3961 cgcctggtcg ggcggcactg cgttgcggta aaacgcctcg gcccccaaat ccgcatcgtc
     4021 cacctttcga acatgaacat cggcgacctg gccgcccctg acttcgaccg gcggctggtt
     4081 gacgacgtca acctggtggc ccccgatctc atcatcgcca gcggcgattt cgtcgaccgc
     4141 acggccgaaa acccggaagc gctctgggaa cggctgccag agtttttcag ccagttcgat
     4201 gctccggcct tcatagcgtg cggcgatcac gacgatctac agcagtactc aaagcacgtt
     4261 gcggtgaacc cgatcggctc ggtcgagctt ggtttcctgc gcgggtttgc gatttatgac
     4321 cgcccgagcc gcccgatcag cgacgacccg ggacagcagc aatggctgga gcgtgccctc
     4381 accgcgccgg gcgacgagcg tctcaagttc gtcgtcgcgc acgacgaccg cccgaacctg
     4441 ctgctgcaat ggcggaagcg cggcgttcta cccaagatga tttccagcac gcggctgggg
     4501 ttgtggatct ctggtagcca tcgtgactgg gacggcgttg agtacgccga catcgtgcgc
     4561 caagcggccc cattgatgta tgtacggacg caccaatcca gcaccgcgac gcgcgacggc
     4621 gcgaccggag tgagccattt tcgcgtgctt gacgtaacca acgaacgtgt ggccgcgccc
     4681 ggctggaatt cgtccgacgt cgcgccgccc tcgctgccca ccggcctgct tacgtgcact
     4741 agcgaatgcc cggccgaaaa cgtggtcgcg gttacggtcg cgaacaacca cgccttccgc
     4801 atcaacggcc tgcaggcgcg cgttctggtc gcgggcgtac cggaagcgcg gccctggtgt
     4861 ttgggcgcgg agttagtctc atcgcacatg attgacggcg tttgggattg tcgcctccgc
     4921 ttcgatgttc cggacaaggg gaccgcacgg gcggtcgtgg gcagcggttc gacgccgctt
     4981 ccgccgcgtt tcacggtccg cttcgacatg ccgacggtgc tgcggctcac tccccagcag
     5041 acccccgagg gcctggaata cgcgagcgcg gcgaatgtta acgcactgat ttatatcacg
     5101 aactccggcg cggtggatgc cgacttcacg ccgctactgc ggctcgatgg cgacacgctc
     5161 agctataagg tcatcgagga gcccggcccg ccggcgctgg cttatcgcct gcatcttccg
     5221 ccggagggct cggtgacgct ccagccggac ttgtcggcct tgcgcacgac gccggggcgg
     5281 cgcgaattgc aggtgtacct gaccggcctg ccggccctgg cgccggcctg ccagccgctg
     5341 acggtgatca cggcgccgtg aggccggttg gcagcgcact cccggggtgt tgtgtcccga
     5401 aggtttcggc gggtgtcctc gcagcgtcgc gatggcaccc ggctcggctg ggatttggaa
     5461 gcagacaacc ggctcattta catgccggcc gcggctggat agactgaaat gtcggagctg
     5521 tttcggcagg caggatggcc agacggctcc tgaacccggg cccatagctc agttggttag
     5581 agcggcggac tcataatccg ttggtcgcag gttcgagtcc tgctgggccc aatcgatatc
     5641 tttgtcctga attgccccga tatgactaac gttgacatta cccttgtttt caaggggttt
     5701 cgcgggtccg ccccgttcgc tcgtccccgc ctgacactcg ctagcagacg ctgactgctg
     5761 cgccttttgc tgcgctttat ccaagcccgc ggccttgcgg aaatcggcgt ccagattcac
     5821 ggac
//
LOCUS       DAOSYS010000223         5821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11548_length_5821_cov_3.843913, whole genome shotgun sequence.
ACCESSION   DAOSYS010000223 DAOSYS010000000
VERSION     DAOSYS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11548_length_5821_cov_3.843913"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..496)
                     /gene="cas2"
                     /locus_tag="PLU99_12465"
     CDS             complement(191..496)
                     /gene="cas2"
                     /locus_tag="PLU99_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOO17906.1"
                     /translation="MWVLAMFDLPVDTKKARRAYAQFRKALLKDGFARMQFSVYIRHC
                     ASEENANVHTQRIQHALPDDGEVRVITITDKQFERMRVFWGKMRQGTEKPPAQLELF"
     gene            complement(539..1510)
                     /gene="cas1"
                     /locus_tag="PLU99_12470"
     CDS             complement(539..1510)
                     /gene="cas1"
                     /locus_tag="PLU99_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOO17907.1"
                     /translation="MIKRTLEISQQPVHLTVRDEQLLLLAKEDAPKRLPADPPGLLAR
                     VPCEDVALLLVEHPGTTYSHAALVTLLRYDAAVVICGRDHLPAGLLLPFSEHTEVVWR
                     IHDQLALRRPLRKQLWRQLVQAKIRAQARNLPSESNIRTRLLALAREVRSGDPQNVEA
                     QAARSYWSAYFGDLFRRDPDGDGINALLNYGYAIVRAAIARAVVAAGLLPALGIHHAN
                     RSNAFCLADDLIEPLRPLVDAQVHELADAGQTELNPSAKAHLLRLLTAEVVTAGQSGP
                     LLVALHRLVASYVHCLQGEARRLEIPVALPCVPDVSSPLDDVAPGPK"
     gene            complement(1549..5646)
                     /locus_tag="PLU99_12475"
     CDS             complement(1549..5646)
                     /locus_tag="PLU99_12475"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOO17908.1"
                     /translation="MPAYLGLDIGSNSVGSAWIDTDEKTITCGISVFPAGVDESDYKR
                     GEPKNVKRRMARRTRITLARRAARKRLLRQKLLAVGLLPRDEGDFKRLLETTDPWELR
                     SKGLDHELTPHEFGRVLLHLAQRRGALGLKLPDAEDGQVDTTDDEGRVKAAIGHVRAQ
                     MLERKARTFGEFIAKVRAERVSPITTEDRRPPARRKGPREYRDPIRNRAGSYEHCADR
                     AMIRDEFTKLWEAQTAFNGETARLLTQANGLRTALDDEAGDSIWLHKGLLFGQRRQSW
                     DLGTLGRCVLEPTERCVPHADMYASRYLVVETVNNLKIIERGREARPLTPEERQKIKD
                     YLSGPLGTERARKKRGDTGPVADKPKTSVTITDVRNLMGWGRATRTSQFRFNIESDPD
                     RVINTGWFSREIIHGAITPAKWAEMPQRLRDGINRAILKYDPDHEGAADKLTLGVMQW
                     GGLNETQADALVAAWKRRPRPDAKRLNMSRRAVRNLLALMDRDEPWPNPRQPGQTCWL
                     TQIEARKLIAEDADFKDVTTGAPLDLQTRVRYATGAKGATARDRYYMQKHLLLKNGKP
                     IFGPDGLPLAEPPPAPLITNPVVRKAIHEVRRHLVDYMLRFERKPDAIVVELAREARM
                     GKVDADRVLFRTRLRNRIRNDIIASFGLGALTSTQQRAAVARVVLCVQQGGICPLCGN
                     QIVTTQITPRMAASGEGCEVAHIIPKACGGDNGLGNIVLAHTKCNRDMGRRTPRQFWE
                     GTFQGGFEEGMRYVEGIYRDIERPKLSEVRSATGQSLWACYFSGRPGRFGPTFDELKI
                     EQFTKSVTDIQEMTPRQEAATKYATRQVMTYLANALYGGQGLPERSGQRRIFATDGLW
                     TSHLRREWRLFFDPHASRTNGLSEEQEHVRKEKDRGDHRHHAIDAVVIALTSPRVQNA
                     WEQRERMADRDGVNTADEVALENYRRAHPLPPPVPFANAQELKQLVHAAVFGSDSLER
                     PVCHRPVKRKLIGELHEAFPRGPVLDRAGSLTDRFTRRISILELDPNHLREPRPETKN
                     EAIKRLAERRRRQTGEDESAARKWARAVVSASGYMPAIVDQPPGKSGIVRDLALRRRL
                     RDCLSAEGLDPDNFTANQIKKLAEAGKVRHVSGVPIRSVVLLWTISDPVLASRWATDH
                     GTGKRRMLFDAVTHQGDARAARAYIGGNNHHIEFRVAPQPDGTEIWSGKIVTAFEVAR
                     RKSQRLRAFRQAGIPKPGDFRKLPKSQRAQFKPTLRAIEAAYPLVDRSNDDAKGGEFV
                     MSLAEGEMLMMKHKVTGKLGYFVVAKLEKRGGNNRDCAVLVPHWDARAAGERKDAEGK
                     RVPDSKREQFTITPVDLKLLAPPDHPHAIKVRATPLGDVYPLQRD"
BASE COUNT          979 a         1814 c         1850 g         1178 t
ORIGIN      
        1 cgattacact ggaacacgcg cgaggcgcgc gcgtagcccc gttgcggatt gctcgcgggt
       61 tcggatcgat tacactcaca gacgagcggc taggcgagat gattatgttg cggattgctc
      121 gcgggttcgg atcgattaca ctattgcccg cgtaacccgc gccgcagaga gaagttggag
      181 ctgaacggcc tcaaaaaagt tcgagttgcg ccggcggttt ttccgtcccc tgacgcattt
      241 ttccccaaaa aacgcgcatc cgctcgaact gcttgtcggt gatcgtgatg acgcgcacct
      301 cgccgtcgtc cggcaaggcg tgctgtatgc gctgcgtatg aacattcgcg ttctcctcgc
      361 tggcgcaatg ccgaatatac accgaaaact gcatccgcgc gaagccgtcc ttgaggagcg
      421 ccttgcggaa ttgcgcatac gcccgccgcg ctttcttcgt atccaccggc aggtcgaaca
      481 tggccagcac ccacataaaa cgatagccgc taagctgcac gggcgcgcgt ctcccgcatc
      541 atttgggccc cggagcgacg tcgtccagcg gggacgaaac gtcggggacg cacggaagcg
      601 ccacgggaat ctccagccgc cgggcttcgc cttgcaggca atgcacatag gatgccacca
      661 gcctgtgtag ggccaccagc agcggaccgc tctggcccgc ggtgaccacc tctgccgtca
      721 gcagccgcaa cagatgggct ttcgcagacg ggttcagttc cgtctgtccc gcgtcggcga
      781 gttcgtgcac ctgggcgtct accagcggcc gtagcggctc gatcaggtca tccgcgaggc
      841 aaaacgcgtt tgagcggttg gcgtggtgga tcccgagcgc cggcagcagg ccggctgcga
      901 caacggcccg ggcgatggcc gcccggacga tggcgtatcc gtagttgagc agggcgttga
      961 tgccgtcacc gtcgggatcg cgccgaaaca ggtcgccgaa ataggccgac cagtagctgc
     1021 gcgcggcctg agcctcgacg ttttgggggt cgccggagcg cacctcgcga gccagcgcca
     1081 gcaaacgtgt gcggatgttt gattcggacg gaaggttgcg cgcctgcgcc cggatcttgg
     1141 cttgcacaag ttgccgccag agctgttttc gcagcggcct gcgcagcgcg agctggtcgt
     1201 gaatgcgcca gaccacctca gtatgctcgg aaaacggcag gagcagtcca gccggcaggt
     1261 gatcgcgccc gcagatgacc acggccgcat cgtaccgtag caaggtgacc agcgcggcgt
     1321 gggagtacgt cgttccggga tgctcaacca gcaaaagtgc gacgtcctcg cacggcacac
     1381 gcgccaaaag tccgggcggg tcggccggga ggcgttttgg ggcatcctct ttcgctaaca
     1441 gcaggagctg ctcatcgcgc accgtgaggt gaacgggctg ctgcgagatt tcgagcgtgc
     1501 gcttaatcat ggcgggcgct atttcatcga caagcgtgcc cgaagaattc agtcccgctg
     1561 aagggggtaa acgtctccga gcggggtcgc ccgaaccttg atggcgtgcg ggtgatcagg
     1621 tggcgcgagg agcttgagat caacgggcgt tatggtgaac tgctcgcgtt tggagtcagg
     1681 gacgcgttta ccctccgcat ctttccgttc tccggccgca cgggcgtccc aatgcggtac
     1741 caacactgcg cagtcccggt tgttgccgcc acgtttctcg agcttggcga cgacgaagta
     1801 gcccaacttg cccgtaactt tgtgtttcat catgagcatc tcaccctcgg cgaggctcat
     1861 cacgaattcg ccacccttgg catcgtcatt tgagcggtca accagcgggt acgccgcttc
     1921 gatggcccgc agcgtcggtt tgaattgggc gcgttgtgac tttggcagct tcctgaaatc
     1981 gccgggcttc ggaatgccgg cctggcgaaa cgcgcgcagg cgttgcgact tacgccgcgc
     2041 gacttcaaag gccgtcacga tcttaccgga ccagatttct gtgccgtcgg gctgtggtgc
     2101 gacgcgaaac tcaatgtggt ggttatttcc tccaatgtag gccctggccg cgcgggcatc
     2161 accctggtgc gtcactgcat cgaacagcat gcggcgcttg ccggtgccgt ggtcagtggc
     2221 ccagcgagaa gccaggacag gatcgctaat ggtccacagc agcacgaccg aacggatcgg
     2281 tacgccggac acgtgcctta ccttgccggc ctcggccagc ttcttgatct ggttagcggt
     2341 aaagttgtcc gggtcgaggc cttcggcgga caaacagtcg cgcagacgtc gacgcaacgc
     2401 cagatcgcgc acgatgccgc ttttaccagg gggttggtcg acgattgccg gcatgtagcc
     2461 gcttgcgctt accacagccc gggcccattt gcgggccgcg ctctcatcct caccagtctg
     2521 ccggcggcgg cgctccgcga gccgcttgat tgcttcgttc tttgtttccg gacgcggctc
     2581 gcgcagatgg ttggggtcca attccaggat gctgatgcgc ctcgtgaacc ggtccgtgag
     2641 cgacccggcg cgatccaaaa cggggccgcg cggaaatgcc tcgtgcaatt cgccaatcag
     2701 cttgcgcttc accgggcgat gacagacggg gcgttccagg gaatcggaac caaaaacggc
     2761 cgcatgcacc agttgtttca gttcctgggc gttcgcaaac gggaccgggg gcggcaaggg
     2821 gtgcgcgcgg cggtagtttt ccagcgcgac ttcgtcggcg gtatttacgc cgtcacggtc
     2881 ggccattcgc tcgcgttgct cccacgcgtt ttgtacgcgc ggcgaagtga gcgcgatgac
     2941 gaccgcgtcg atcgcgtggt gccgatgatc gccgcggtcc ttctcctttc gaacgtgttc
     3001 ctgctcctcg ctgaggccgt tggttctgga ggcgtgcgga tcgaagaaca ggcgccactc
     3061 gcgtcgtagg tggcttgtcc acagtccatc cgttgcgaaa atccggcgtt ggccgctacg
     3121 ttcgggaaga ccctgccctc cgtagagtgc gttggcgaga taggtcatca cctggcgggt
     3181 cgcgtacttg gtggccgcct cctggcgggg cgtcatctcc tgaatgtccg tgacgctctt
     3241 cgtgaattgc tctattttca gctcatcaaa cgtcggacca aatcgaccgg ggcgtccaga
     3301 aaagtagcag gcccaaagcg actggccggt ggcgcttctc acttccgaca gcttgggcct
     3361 ttcgatgtcg cggtagatcc cctcgacgta gcgcatgcct tcctcgaacc ccccctggaa
     3421 ggtgccttcc caaaactggc gcggcgtgcg ccgccccatg tcccggttgc acttcgtgtg
     3481 cgcgagtaca atgttgccca aaccattgtc cccaccgcag gccttgggaa ttatgtgggc
     3541 cacctcgcac ccttcaccgc tggcggccat cctgggggtg atctgagtcg tcacgatctg
     3601 attgccgcac agcgggcaaa tgccgccctg ttgcacacac agcacaaccc gggcgacggc
     3661 ggcgcgttgc tgcgtggacg taagcgcgcc caggccaaaa ctggcgatga tgtcgttgcg
     3721 aatgcggttg cgcagacggg ttcggaacag aacgcggtct gcatcaacct tccccatgcg
     3781 cgcctcacgc gccagctcga cgacgatcgc gtccggtttg cgctcgaagc gcagcatgta
     3841 gtccaccaga tggcggcgca cctcgtgaat agccttgcgc accacggggt tggtgatcag
     3901 cggtgccgga gggggctcgg ccaggggcag tccgtcgggg ccgaagatcg gcttgccgtt
     3961 cttcagcaga aggtgcttct gcatgtagta gcggtcgcgg gcagtggcgc ccttggcgcc
     4021 ggtggcgtag cgcactctgg tctgcaggtc gagcggcgcg ccggtggtca catccttgaa
     4081 atccgcgtct tcagcgatca gcttgcgcgc ctcgatttgc gtcaaccagc aggtctgccc
     4141 gggttggcgc gggttcggcc agggttcgtc gcggtccatc agcgccagta gattccgcac
     4201 ggcgcggcgg ctcatgttca ggcgtttggc gtccggacgc gggcggcgct tccacgcggc
     4261 gaccagtgca tccgcctgcg tttcattcag tccgccccat tgcattacgc cgagtgtgag
     4321 cttgtcagca gcgccctcgt gatcgggatc atattttaaa atggcgcgat taattccgtc
     4381 gcggaggcgc tgcggcattt ctgcccactt ggcgggcgtg atggcgccgt ggatgatctc
     4441 gcggctgaac caaccggtgt tgatgacccg atccgggtcg gactcgatgt tgaaccgaaa
     4501 ctgcgaggtt ctggtggcgc ggccccagcc catcagattt cgcacgtcgg tgatagtgac
     4561 actggttttc ggcttgtcgg cgacggggcc ggtatcgcca cgctttttgc gagcccgctc
     4621 cgttcccagc ggtccgctga ggtagtcctt gatcttctgt cgctcttcgg gcgtcagtgg
     4681 gcgggcttct ctgccacgct caatgatttt caggttgttg accgtctcga ctacgaggta
     4741 gcgcgaggca tacatgtccg cgtgaggtac gcagcgctcg gttggctcca gaacacatcg
     4801 tccgagcgtt cccaggtccc aggattgccg gcgttggccg aacagcaggc ctttgtgcag
     4861 ccagattgag tcacccgctt cgtcatccag cgcggtgcgc aagccgtttg cctgagtcag
     4921 gaggcgcgcc gtctctccgt tgaatgcggt ctgcgcttcc cacagcttag tgaactcgtc
     4981 tctgatcatg gcacgatcgg cgcagtgttc gtaacttccc gcccggttgc ggatcgggtc
     5041 gcgatactcg cgcgggccct tgcgccgggc gggcggacgc cggtcctcag tcgtgatggg
     5101 gctgacgcgc tcggcacgca ctttcgcgat gaattcgccg aaagtgcggg ccttcctttc
     5161 cagcatttgt gcgcgcacgt ggccgattgc ggccttcacg cgtccctcgt cgtcagtcgt
     5221 atcgacttgg ccgtcctccg cgtctggcag cttgagacct aacgcgcctc tacgctgcgc
     5281 caggtgcagc agcacgcgcc cgaattcgtg tggcgttagt tcgtggtcga gacccttgct
     5341 tcgaagctcc cacgggtctg tcgtttccag cagacgcttg aaatcaccct cgtcacgcgg
     5401 cagcagcccg actgccagca atttctgccg caggaggcgt ttgcgcgcgg cgcgccgtgc
     5461 gagggtgatg cgggttcggc gagccatgcg gcgcttgacg ttcttcggct caccgcgctt
     5521 atagtcggat tcgtcaacac cggcggggaa tacgcttatg ccgcatgtta tggtcttctc
     5581 gtcggtgtca atccacgccg aaccgacgga attactgccg atgtccaagc ccagatatgc
     5641 aggcatgctt cgctccacca caaaagagaa gatgacgcct aaacgtcctt gacagcgact
     5701 ctgacgtgca attaaaatta tgtcaagtgt aatcgatccg ggcccgcgat tttacgcaac
     5761 aagagaaaat cgcaggcacc gccttcgggc gaccgccgca gctcgtgcag cccacgacct
     5821 g
//
LOCUS       DAOSYS010000224         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11551_length_5820_cov_5.498179, whole genome shotgun sequence.
ACCESSION   DAOSYS010000224 DAOSYS010000000
VERSION     DAOSYS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_5820_cov_5.498179"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1683)
                     /locus_tag="PLU99_12480"
     CDS             complement(124..1683)
                     /locus_tag="PLU99_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOO17909.1"
                     /translation="MPAHDLQSFVAELERRGWLKRISAPVDPRLEICEITDRVTKSGG
                     PALLFENVVGSRFPLLINAFGTRERMCLALGAASFEEIAERVRHVLRPEVPTTLVQKL
                     KKLPELAQLASLAPRIVKGGVCQEVVHTGDADLFSLPVMKCWPLDGHPELCGGSPAQV
                     ASAGVSSVNAGSAASAASPAPSADVGRYITLGAVFTKNPDTGERNIGMYRVQLFEPKL
                     AAMHWHPHHDGARHFRRYQQRGRRMPVAIALGGPAVLPYAATCPLPPGVDECLLAGFL
                     QGRGIDLVPCVSQPEIEAPASAEIVIEGYIDPGEDLVLEGPFGDHTGFYSLPDFFPRL
                     HVTALTHRREPIYPSTIVGRPPQEDYWFGKATERIFLPVLQAIVPDLIDYNLPMFGCF
                     HNCVFVRIKKEYPYQARRVMNAIWGAGQMAFSKIIVVVDEHVDVQNEQEVLFQLCSNV
                     DPRRDILFTDGPLDILDHAAPYCGVGSKMGIDATRKLPGEGQVRQWPPEIEMSREIKD
                     LVSRRWREYGL"
     gene            1937..2698
                     /locus_tag="PLU99_12485"
     CDS             1937..2698
                     /locus_tag="PLU99_12485"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOO17910.1"
                     /translation="MVLAHVAAFILLLVVRTEPDGPALANSLGLSDKSARLWAIFTHP
                     FATPSLLSLLITVFILWTLAARVERLFGWRRMLVLYACGNILAGTAFYALARLSPPLA
                     TAGLDSPTGAFAAWVVMAYRGMGGQMVSLFGGAYRLSRVIAVTLAIMTGLMLMLRGFG
                     AIGWLAAIAAGACAEPLLASLSNSMATLPRRRRVVRPSIPRDDSAREQREPDPYDVDD
                     ILAKISRGGMESLTAADRKRLELARQAKLRESEWA"
     gene            complement(2751..5228)
                     /locus_tag="PLU99_12490"
     CDS             complement(2751..5228)
                     /locus_tag="PLU99_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOO17911.1"
                     /translation="MSVLLIASALQVPAKGQDEPPASLPADAGPPASMAGFSDEGAFD
                     IYRAEERLVRTTFTWRADGSYETQTVVSLGGQSMTFTGKITPDAEGRFWRMEFDLPTG
                     QQVFEREGAQVRFTHKEVKRTFTLRQDAVPFENMSPAFMSLAVRRYDRAAGGQQTMPL
                     LMLNALAVMDGSLEFLDSVERSVGGRDLKLDRYKFAIPGVDLTVWADETGKVYFVDVP
                     AQRAYYVREGYEELHVAEEDDPKLSQPVFEVIEERGVEVPMRDGVKLSTDIYRPKTDD
                     KVPVILVRTPYKKEMSELDGKFYARRGYAVAVQDCRGRFSSPGEWEPFVNEKTDGYDT
                     IEWLAAAPWSTGKVGMLGGSYLGWVQWQALSQNPPHLTTIIPNVSPPDPFFNFPYEYG
                     VFYMYAGIWWADIVASEATGDLTGVKMSAVSEKKLGRLLRDLPVIELDKKVLGGENQY
                     WRAWIKNNTNNEYWARASFSEELEGVTIPVFHQSGWFDGDGIGSKLNYAALARHGKST
                     QKLILGPWGHTAEARRMLGDTDFGPEAAPNLPRMYLRWFDHWLKGIDNGIDREPLVSL
                     FVMNSNKWVHGGRYPLEGTQFEKWYLTSGGSANTSKGDGRLTREAPAENPPDRYIYDP
                     ADPTPDPDFFEESEDEEKQEKSIEELKARAKKRHEQVTDARNDILVYTTEPFDEPYTF
                     VGPVSARLYAASSAKDTDWFMRLIEIKADGEVNTLVGGRFRARFRESLSRPALLEPGQ
                     VYAYDIDLWQTGLTVQTGSKLRVEVASASFPMFSRNLNTGGHNEMETEYVAAEQTIYH
                     DAEHPSHVLLPMIPNEMLSRGSDGSVK"
     gene            5580..>5820
                     /locus_tag="PLU99_12495"
     CDS             5580..>5820
                     /locus_tag="PLU99_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17912.1"
                     /translation="MMGTRWLIPQLSLSAALVLCGVTVAQEQAPPQLQVTAVAPNIHM
                     LAGAGGNICLFIGDDGALLIDGGVTPRAPNVLAAVK"
BASE COUNT         1042 a         1903 c         1776 g         1099 t
ORIGIN      
        1 attgcagaat cacgggcctg cgcgtgttgg aaggcagagc cggggcgtgt cgccccgtag
       61 tttgtgcaca atgcgtggtc gtgagcccgc cagcaatcac cggggcggca cgccccggct
      121 ccgctacaac ccgtactcac gccagcggcg cgacaccaga tccttgattt cccgcgacat
      181 ttctatttcc ggcggccatt ggcgaacctg cccctccccc ggcagcttgc gcgtggcgtc
      241 gattcccatc ttgctgccaa ccccgcagta cggggcggca tggtccagaa tgtcgagcgg
      301 gccgtcggtg aacagtatgt cgcgccgcgg atcgacgttg ctgcaaagct gaaacagcac
      361 ttcctgctcg ttctgcacgt ccacgtgctc gtccaccacc acaatgatct ttgagaacgc
      421 catctgccct gcgccccaga tcgcgttcat gacgcgccgc gcttggtacg ggtattcctt
      481 cttgatgcgt acgaatacgc agttgtgaaa acacccgaac atcggcaggt tgtagtcaat
      541 cagatcgggc acaatcgcct gcaacaccgg caagaatatg cgttccgtgg ctttgccgaa
      601 ccagtagtct tcctgcggcg gacgcccgac gattgtggaa ggataaatcg gctcgcggcg
      661 gtgcgtgagc gccgtcacgt gcaaccgcgg aaagaaatcc ggcaaactgt aaaagccggt
      721 gtgatcgcca aacgggcctt ccaaaaccag atcctcgccc ggatcgatgt agccctcgat
      781 cacgatttcc gcactggccg gcgcctcaat ctccggctga ctgacgcagg gcaccaaatc
      841 gatgccacgc ccctggagaa agccggccag caggcactcg tccacacccg gcggcagcgg
      901 acacgtggcg gcgtacggca acacggccgg cccgcccagc gcgatcgcga ccggcatgcg
      961 ccgcccacgc tgctgatagc ggcgaaagtg acgcgccccg tcgtggtgcg gatgccaatg
     1021 catcgccgcc agcttgggct cgaaaagctg gacccggtac atgccgatgt tgcgctcgcc
     1081 ggtatcaggg ttcttcgtga aaaccgcccc cagcgtgata taccggccca catcggcgct
     1141 tggcgccggc gacgccgcac tcgccgcgct gccagcgttg accgacgata cgcccgcact
     1201 cgccacctgg gcaggcgacc cgccgcaaag ctccggatgc ccgtccagag gccagcactt
     1261 catgaccggc aggctgaaca agtccgcgtc gcccgtgtgt accacctcct ggcaaacgcc
     1321 gcctttgaca atgcgcggcg ccaagctggc cagttgagcc agttccggaa gcttcttcaa
     1381 cttctgcacc agcgtggtcg gcacttccgg ccgcagcacg tgcctgacac gctcggcgat
     1441 ttcctcgaag cttgcggccc cgagcgccag gcacatccgc tcccgcgtgc cgaaggcgtt
     1501 gatgagcagc ggaaagcgac tgccgacgac gttttcaaag agcagcgccg gcccgccgga
     1561 cttggtcacg cggtccgtaa tttcgcagat ttcaagccgc ggatcgacgg gagctgaaat
     1621 gcgtttcagc cagccgcgcc gctcgagttc ggccacaaag ctttgcagat cgtgggcagg
     1681 caagtcgaat tctccgcgaa caatctgtaa gttggggcag tatacggaaa cgtagtcgcc
     1741 tcgcgtagtg ccggcttcgg aatcgcgatg cagcggggat aaaatacggc gcgggcgact
     1801 ggaggcccgc ggaaacgttg ccagcgggag ttgcgccgtg gcctggcgag atcgagacta
     1861 ttcaaaagat gagccgtgga cacagcatgg ccccctacgg tccagcatac ggtggccggg
     1921 cgggctgacg gcaacattgg tactagcgca cgtcgctgcc ttcattctgc tattggtggt
     1981 cagaactgag ccagacggcc ccgcgctggc gaacagtctg gggctgtccg acaagagcgc
     2041 ccgcctgtgg gccatcttca cccatccgtt cgcgacgccc agtctactct cattgcttat
     2101 caccgtgttc atcctgtgga ccctggcggc acgcgtcgaa cggctattcg gctggcgccg
     2161 catgctggtg ttgtatgcgt gcggcaacat cctggccgga accgcctttt acgccctggc
     2221 gcgtctgtcg ccgccgctgg cgactgccgg cctggacagt ccgaccggcg catttgcggc
     2281 gtgggtcgtc atggcctacc gcggcatggg cggccaaatg gtttcgctct tcgggggagc
     2341 gtaccgcctc tcgcgcgtaa ttgccgtgac gctggccatc atgacgggac tgatgctcat
     2401 gctacgcggc ttcggcgcca tcggctggct ggccgcgatc gccgccggcg cgtgtgccga
     2461 accgctcctg gcgagcttga gcaatagcat ggcaaccctg ccgcgccgcc gccgcgtggt
     2521 gcggccgagc attccgcgtg acgattccgc gcgcgaacag cgcgagccgg acccgtatga
     2581 cgtcgacgac atcctggcca agatcagccg cggcggcatg gagtcgttga ccgcggccga
     2641 tcgaaagcgc ctggaactgg cgcggcaagc taagctgcgc gagtcggaat gggcgtaacg
     2701 atcgctggag tatcgcggcg gcgcatggtg cgccgcaagc gggccgcgcc ctatttcacg
     2761 cttccatctg acccgcgcga cagcatttcg ttcggaatca tcggcaagag cacgtgcgac
     2821 ggatgctccg catcgtggta gatggtctgc tcggccgcaa cgtactcggt ttccatttcg
     2881 ttgtgcccgc cggtgttcag gttacgggag aacatcggga atgacgccga ggcgacctcc
     2941 acgcgcagct tcgatcccgt ttgcactgtc aggccggttt gccagaggtc gatgtcatac
     3001 gcgtatacct ggcccggttc gagcaatgcc ggcctgctca gcgactcccg gaaacgggct
     3061 cggaagcgcc cgcctacaag cgtgtttact tcgccgtccg ccttgatctc gatcaagcgc
     3121 atgaaccagt cggtatcctt cgcggaagag gcggcataca gccgcgccga gaccggaccg
     3181 acgaaagtgt acggctcgtc gaacggttcg gtcgtgtata ccagaatgtc attgcgagca
     3241 tcggtaacct gttcgtggcg tttcttggcg cgggctttca actcctcgat tgacttctcc
     3301 tgcttttcct catcttctga ttcctcgaag aagtccggat cgggggttgg atcggccgga
     3361 tcatagatgt aacggtcagg cgggttttcg gccggcgctt cgcgcgtcag ccgtccgtcg
     3421 cctttggatg tgttggcgct tccgccgctg gtaaggtacc acttctcgaa ctgcgtgccc
     3481 tccagcgggt agcggccgcc gtgaacccat ttgttgctgt tcatcacgaa cagactcacg
     3541 agcggctcac ggtcaatgcc gttatcgatg cccttcagcc aatggtcgaa ccagcgcaag
     3601 tacatgcgcg gcaggttggg cgcggcctcc ggtccgaagt ctgtgtcacc cagcatgcgc
     3661 cgcgcttcgg ccgtatgtcc ccaaggcccc agtatcagct tttgcgtgga tttgccgtgc
     3721 ctggcgagag cggcgtaatt gagcttgctg ccgatcccgt cgccatcgaa ccagcccgac
     3781 tggtggaaaa cgggaatcgt gactccttcc aactcttcac tgaagctggc ccgcgcccag
     3841 tactcgttat tggtattgtt cttgatccac gcgcgccagt actggttctc gccgcccagc
     3901 actttcttgt ccagctcgat caccggcagg tcacgcagca gccggccaag cttcttctcc
     3961 gacacggcgc tcatctttac gcccgtcaaa tcgccggtcg cttcgctggc cacaatgtcc
     4021 gcccaccaga tgccggcgta catgtagaac acgccgtatt cgtacggaaa gttgaagaac
     4081 gggtccggcg gcgagacgtt cggaatgatc gtggtcaggt gcggcggatt ctgcgacaac
     4141 gcctgccact gcacccaccc caggtacgaa ccgccgagca tgcccacttt gcccgtgctc
     4201 cacggagcgg ccgccagcca ctcgatcgtg tcgtagccgt cggttttctc gttgacgaac
     4261 ggctcccact cgcccggcga actgaagcgc ccgcggcaat cctgaacggc cacggcgtag
     4321 ccgcgccggg cgtagaactt gccgtccagt tcggacattt ccttcttgta cggggtccgc
     4381 acgaggatga ccggcacctt gtcgtccgtc ttgggccggt aaatgtcagt cgagagcttc
     4441 acgccgtcac gcattgggac ttcgacgccg cgctcttcga tgacctcgaa caccggctgc
     4501 gacagctttg ggtcgtcctc ttcagcgacg tgcagctctt cgtagccctc gcggacgtag
     4561 taagcccgtt gcgccggcac gtccacgaag tacaccttgc cggtttcgtc ggcccacacg
     4621 gtcaggtcca cgcccggaat ggcgaactta tagcggtcga gtttaaggtc ccgcccgccc
     4681 accgaacgct ctaccgaatc gaggaattca agcgaaccgt ccatgacggc cagcgcgttc
     4741 agcattaaca gcggcatggt ctgctgcccg ccggccgcgc gatcgtaacg ccgcaccgcc
     4801 aacgacatga aggccgggct catgttctcg aacggcaccg cgtcctgccg cagcgtgaac
     4861 gttcgtttta cctccttgtg cgtaaaacgc acctgggccc cttcgcgctc gaacacctgc
     4921 tgaccggtag gcaggtcgaa ctccatgcgc caaaagcggc cctcggcgtc cggcgtgatc
     4981 ttgccggtga aggtcatgct ctgcccgccg agactgacga cggtttgcgt ttcgtaactg
     5041 ccgtcggcac gccaggtgaa ggttgtgcgg accaggcgtt cttcggcgcg gtagatatcg
     5101 aaggcgcctt cgtcggaaaa acccgccatg gacgcgggcg ggccggcgtc ggccggcaac
     5161 gatgcgggcg gctcgtcctg tcctttggcc gggacctgca acgcgctggc aatcagcagg
     5221 acagacatca aacagcgaaa tcgtgaaaac atcacagcac ctcagccaaa gagcccttat
     5281 caacgcctcc ccgcttcagc cggagagcac gcggcgcgcg ctgagcggcc acagcgcact
     5341 gcagagccat tcttagctcg ccgtcgacgg atgaaaaggg cgacgctcgc ggtttaagca
     5401 ctggagttgt cgtttatcgt cagatacgcg ccggctgtgg cgcaacgcgg catctctgcc
     5461 ggcgcgcttc cacgcgcaat taacgcgtgc ctggacacgc cgggtagctc tgcctaatct
     5521 gcggttgaca agtgcttatc cggcgcagag tagcgcggat taatcgcagg gagacgagca
     5581 tgatgggcac acgttggcta attccacagt tgtcgttaag cgccgctctg gtgctctgcg
     5641 gggtgacggt tgcacaggag caagcacccc cgcagctcca ggtaacggcc gtcgccccga
     5701 acatccacat gctggcgggg gccggtggga atatctgcct attcatcggc gacgacggcg
     5761 cgctgctgat tgatggcggc gtcacgccac gggcgcccaa tgttcttgcg gccgtgaagg
//
LOCUS       DAOSYS010000225         5787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11633_length_5787_cov_4.596825, whole genome shotgun sequence.
ACCESSION   DAOSYS010000225 DAOSYS010000000
VERSION     DAOSYS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11633_length_5787_cov_4.596825"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLU99_12500"
     CDS             <1..402
                     /locus_tag="PLU99_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOO17913.1"
                     /translation="ATLGCTRIGSGTKFSNLIAIGHGAQIGADCLFVAQVGIAGSATV
                     GRHVTMAGQAGVVGHIRIGDNATVGARAGVTNSVPDGETVLGQPAIPIREMRRQIAYV
                     QRLPDLNSRVKEQEKRIAKLEEQLRNCQRST"
     gene            527..1009
                     /locus_tag="PLU99_12505"
     CDS             527..1009
                     /locus_tag="PLU99_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO17914.1"
                     /translation="MPEKVNAVPEGFHTVTPSLVVRKAAEAIDFYRRAFGAEEICRMR
                     GPDGKSVVHAELKIGDSIIFLSDEFPEMGSRGPESLGGTATTIHLYVPDVDAVFQQAI
                     AAGAQSLMPVQDQFWGDRFGTLVDPYGHRWSISTHIEDVSPEECERRAAEAFANKNCG
                     "
     gene            1237..2379
                     /locus_tag="PLU99_12510"
     CDS             1237..2379
                     /locus_tag="PLU99_12510"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOO17915.1"
                     /translation="MHTKVISTIQPGTYGASIAEAAAALREGALVLFPTETVYGVAAN
                     AAKPDALRRLRELKKSAVSQRPFTVHLGRKADAGKYLTGSSALLRRLSRKCWPGPLTM
                     VVQEAAPERAEIFRVCPREQQRELYYEGRVGLRCPDHPAALELIAQVDVPVVATSANA
                     RGQIPPTEFEAAYNNLEGQVEYALNAGSTKLGAASTVINVHDHQWSVQRAGPIEERTL
                     RRLSRSVILMVCSGNSCRSPMAEYMFRHKLAESLGMSPEDLPAAGYEVLSAGTAACPG
                     CEASPGALLEMRRRGMDLSAHRSQPLTIELIQQAERIYVMTPEHRAMVLDLAPGAADV
                     VQLLDPRGAITDPMGGGPEEYSKTADRLERAIEARLKEFLDEDRNW"
     gene            2360..2848
                     /gene="rpiB"
                     /locus_tag="PLU99_12515"
     CDS             2360..2848
                     /gene="rpiB"
                     /locus_tag="PLU99_12515"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOO17916.1"
                     /translation="MRIAIGSDHRGHEAKQRIKALLLEMGIKLEDHGGQSGETCDYPD
                     IALAVAHDVAEGRADRGILFCGSGIGMSITANKVTGIRAALCHDDLTAQMSRRHNDAN
                     VLCLPADLVGDALMQSIVRVWLDTEFEGGRHQRRVDKIRAYESERNGVAHKTPPPPKA
                     AS"
     gene            complement(2859..3299)
                     /locus_tag="PLU99_12520"
     CDS             complement(2859..3299)
                     /locus_tag="PLU99_12520"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HOO17917.1"
                     /translation="MNPDKIKTATPSRSGPAEKVFTAAEANAALVLVQKVVQDLVKKY
                     RHLLSLRDKRHRLANSVGNAEQIETLNAQIEYTTAALNELHAELIGIGCVLKDWSEGL
                     VDFPAMHRGRRVWLCWRLGEQRVTHWHDLHGGYVGRQPIGPDFD"
     gene            complement(3619..3957)
                     /locus_tag="PLU99_12525"
     CDS             complement(3619..3957)
                     /locus_tag="PLU99_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO17918.1"
                     /translation="MPFEQWSETVILAELADDPQFTDDLSAIIEQCTDNARNDVLLSF
                     AHVGYLNSSNIAKLLKLRKLVTVTNQRKLKLCGINTRVWGVFLVTGLDHIFEFVDDVP
                     SGLAALQIEH"
     gene            complement(4158..4748)
                     /locus_tag="PLU99_12530"
     CDS             complement(4158..4748)
                     /locus_tag="PLU99_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOO17919.1"
                     /translation="MLNRLYMAVRVISARRALTLLYQDSAEVVAVEDGQFLSYDFDDW
                     VEVSRAKRRFEPEQHDWVRTVHFQIAVPKIVRLLVYDRLPRTSVKLNRRNLFARDQNR
                     CQYCGKRFPSSELSLDHVVPRSRGGGNTWENIVCACVRCNVRKGGRTPSEASMKLIAV
                     PRRPHRSPVLTIKLSDSRYASWKQFLDFAYWDVELK"
     gene            5021..>5787
                     /gene="lpxI"
                     /locus_tag="PLU99_12535"
     CDS             5021..>5787
                     /gene="lpxI"
                     /locus_tag="PLU99_12535"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOO17920.1"
                     /translation="MESSLATSRSPLGLIAGEGEFPKLVARGARAAGRPVAIVALRGA
                     ADAELRDLAERFCWRGFVQVGRWIRALRRAGCREVIMAGRVRKTQMFAGPRWLQYLQY
                     LPDLTSIRLWYFHAPDKRNDSLLAAVAEEMQRKGLTLIDSTTYCRQALAPLGVLTPFP
                     PPAAVLEAADFAWPIAKEIARLDIGQSVAVKEREIIAVEAIEGTDRLIERAGQLCPQG
                     GWTLVKVAKPNQDMRFDVPTIGPRTIENLKRARARGLV"
BASE COUNT         1094 a         1801 c         1838 g         1054 t
ORIGIN      
        1 gccacgctgg gttgcacccg gatcgggtcc ggcaccaaat tcagtaatct gatcgccatc
       61 gggcatggcg cccagatcgg cgcagactgc ctgtttgtgg cccaggtggg gatagcagga
      121 tcggctacgg tcgggaggca cgtgacgatg gccggccagg ccggtgtggt gggtcacatt
      181 cggattggcg acaacgcgac ggtcggcgcc agagccggcg tgacgaacag cgtgccggat
      241 ggcgaaacgg tgctgggcca gccggcgatc ccgattcgcg aaatgcggcg ccagattgca
      301 tacgtgcaac gcctgccgga tctcaatagc cgagtcaagg agcaggaaaa gcgtatcgcc
      361 aagctggaag agcagctccg caattgccaa cgttccacct gatatggtga accgccgtct
      421 gatggtgaac ggccgcggtc tgaatagaag ttcatgttga ctcagagcgc gccggccgct
      481 ttagaatctc tttagaacca ggccacacca accaaggagg cagaacgtgc cggagaaagt
      541 gaacgctgta cccgaggggt ttcataccgt cacgccgtcg ctggtcgtgc gtaaggcggc
      601 tgaggccatc gacttctatc gccgtgcttt tggcgccgaa gagatatgcc ggatgcgcgg
      661 acccgacggc aagtcggttg tgcacgccga gttgaagatc ggcgattcga tcatcttcct
      721 gagcgatgaa ttcccggaga tggggtcacg cggcccggag tcgctaggcg gcacagcgac
      781 caccattcac ctgtacgtgc ctgacgtcga cgccgttttc cagcaggcca tcgcggccgg
      841 cgcgcaatcg ctaatgcccg tgcaggacca attctggggc gaccgatttg gcacgttggt
      901 cgatccatac ggccaccggt ggtcaatcag cacgcatatc gaggacgttt cgcccgagga
      961 atgcgagcgt cgcgcggctg aggcgttcgc taacaaaaac tgcggctgaa aagccgtcac
     1021 ggccggtcat cacgctgtcc aacctacaag tgggtgggcc ggttctccgc tacggcgaag
     1081 tcggccgtgc acctcgacca cctcgcgact cggaataaca tcaaagggcg gcttccggct
     1141 gagcgatagc ttggccggcc gggccgcgcc atgaccggta aacttgacac gcgccgtaca
     1201 tgcccggaca atttcatccg gcagcgggac ccccaaatgc atacgaaggt cattagcacc
     1261 atacaaccgg gtacttacgg agcttcgatc gcggaagcgg ccgcggcctt gcgcgaaggg
     1321 gccctcgtgc ttttcccgac cgaaacggtc tatggggtcg ctgctaacgc ggctaagccg
     1381 gatgccttgc gacgcttgcg agagctgaag aaatcggccg ttagccagcg gcccttcacc
     1441 gtgcatctag gccgcaaggc tgacgcgggc aagtatctga cgggctcgtc ggctctacta
     1501 cggcggctgt cgcgaaagtg ctggccgggg ccgcttacga tggtggttca ggaggctgca
     1561 ccggagcggg cggagatttt ccgcgtgtgt ccgcgggagc agcagcggga gctgtactat
     1621 gaggggcgcg tggggttgcg ttgcccggac cacccggcgg ctttggagct gatcgcccag
     1681 gtcgacgtcc cggtcgtggc gaccagtgcc aacgcccgcg ggcagatccc gcccacggaa
     1741 ttcgaagcgg cctacaacaa cttggaaggc caggtcgagt atgcgttgaa cgcggggagc
     1801 actaagttgg gggcggcctc aaccgtaatc aacgtccacg atcatcagtg gagcgtgcag
     1861 cgcgccggcc ccatcgagga gcgcacgttg cggcggctct cgcgcagtgt cattctcatg
     1921 gtctgcagcg gtaattcgtg ccgctcaccc atggccgaat acatgttccg gcataagcta
     1981 gccgaaagct tggggatgtc gccggaggac ctgcccgccg ccggttacga agtgctttcg
     2041 gccggaacag cggcctgccc cgggtgcgaa gcgtcgcccg gcgcgctgct ggaaatgagg
     2101 cgtcgcggga tggatttgag cgcgcatcgc tcgcaaccgt tgacgatcga gctgatccag
     2161 caggctgagc gaatttacgt catgacgccc gagcaccgtg ccatggtgct ggacctggcg
     2221 cccggcgcgg cggacgtggt ccagttactg gatccgcgtg gggccataac cgaccccatg
     2281 gggggcggtc cggaagaata ttcgaagacg gccgaccgtc tggagcgggc catcgaagcc
     2341 cggctcaagg agttcctgga tgaggatcgc aattggtagc gaccatcgcg gacacgaagc
     2401 taaacagcgc atcaaggccc tgctgctgga aatgggcata aagctggaag atcatggcgg
     2461 ccaatccggc gagacctgcg actaccccga tatcgcgctg gcagtggcgc atgatgtggc
     2521 cgagggccgg gccgatcggg gcattctgtt ttgcggcagt ggcatcggga tgtccatcac
     2581 ggcgaacaag gttacgggca tccgcgccgc gctatgccat gacgacctga ccgcgcagat
     2641 gtcgcgccgt cataacgacg ccaatgtgct ctgcctgccg gcggacctgg tgggtgatgc
     2701 tctcatgcag agcatcgttc gcgtgtggct cgacacagaa ttcgagggtg ggcggcatca
     2761 gcgccgcgtg gacaagatcc gggcctacga aagcgaacga aacggcgtgg cacacaaaac
     2821 gccgccaccc ccgaaagcgg cttcctgaca ctccccgctc agtcgaaatc cgggccgatc
     2881 ggctgacggc cgacgtagcc gccgtgcaga tcgtgccaat gcgtgacgcg ctgctcaccc
     2941 agccgccagc acagccacac ccggcgcccg cggtgcatgg cggggaaatc aaccagccct
     3001 tcggaccaat ccttgagcac gcaaccaatg ccgatcagtt cggcgtgcaa ttcattcagc
     3061 gccgcagtcg tgtactcgat ctgcgcgttt aacgtctcga tctgttccgc gttacccact
     3121 gagtttgcca agcgatggcg cttgtcgcgc aggctcagca gatgcctgta tttcttgacc
     3181 aggtcctgga cgaccttctg caccaggaca agggcggcgt tggcttctgc tgcggtaaag
     3241 accttttcgg cgggtccgct ccgggacggc gtcgcggtct tgattttgtc tgggttcatc
     3301 cctgtcctcc gcgctgcctg acgcggaccg gctcaaagta agcctagacg caccgcaact
     3361 cttttcaatg tagacgccga tgttacccga tttcgcgttc gacattcagg ccatagaccc
     3421 agtcggccgc tgaagatgcg caacgctaaa caacgtatcc aggctcacgt gaagtcgtcc
     3481 tcgtcgcgcc gcaccgcgct gccgggagac gggaccgcca cggatgccgc tctgtctgag
     3541 cggtttgtcg ggagtttgtc atccggcgaa caagacgcag accaggagaa agagaagcgt
     3601 taaccgcaac ggcggcgttc agtgctcgat ctgcagggcc gccaatccgc tgggcacgtc
     3661 atccacgaat tcaaagatgt gatccagccc ggtcaccagg aacacccccc agacgcgcgt
     3721 gttaatcccg cacagcttga gcttgcgctg gttcgtcacg gtgaccagct tgcgcaattt
     3781 cagcagcttg gcgatgttgg aggagttcag gtagcccacg tgggcgaaac tgagcagcac
     3841 gtcgttccgg gcgttgtccg tgcactgttc gatgatcgcg ctcaggtcat ccgtgaactg
     3901 cggatcatcg gccaactccg ccagaatcac cgtctcggac cactgctcga agggcatcgt
     3961 ctcgctacct ccgccaggag ctttacctct tccaggtgtg cggaccataa tctcgcagcg
     4021 ctacctggtc aacgccggtt tcgcgcctcg cgctcccgtc cgcatgtggg cgcagcgcga
     4081 gcgacactga acgctaagat cggcagaaag ttcggcttcg ctgccgggcg cttgggcggc
     4141 tgggcgctca cgaacgctta cttcagctcc acgtcccagt acgcgaaatc caggaactgc
     4201 ttccaactcg cgtaacgcga atcggatagt ttgatcgtca ggaccggcga gcgatgcggc
     4261 ctgcgcggca cggcgatgag cttcatgctg gcctcgctcg gcgtgcgacc gcccttgcgc
     4321 acattgcagc gcacacaggc acagacgatg ttctcccaag tgttgccgcc gccgcgggag
     4381 cgcggcacga cgtggtccaa gctcagctcg ctgcttggga aacgcttacc gcaatattgg
     4441 cagcggtttt ggtcacgcgc aaagaggttg cgccgattga gcttaacgct ggtgcgcggc
     4501 aagcggtcgt agaccagcag gcgcacgatc tttggcaccg cgatctgaaa gtgaaccgtg
     4561 cgcacccaat catgctgttc cggctcaaac cgccgcttgg cccggctgac ctccacccaa
     4621 tcgtcgaagt cgtacgacag aaactgcccg tcctcgactg cgaccacctc ggccgaatcc
     4681 tgatacagca gcgtcagcgc ccgacgcgca ctgatcacgc gcaccgccat gtacaggcgg
     4741 ttcagcatca gtacactgct ggatagtgcc gaggcctcgg cagtcatgcg gacggccttt
     4801 cgtcagactc cgtaggtagc ggctcaccaa gctgtctcca cgaattgtag tggaatcggg
     4861 taacggacac aacgggtaaa ttaacctccg catccacttt tactgcctgc caggcggtat
     4921 aatggcgggc ggcgcgtggt gctctggtgt cgattaacca gcgctctagt ttgctgtcgg
     4981 cctgaaatgc ttgctcggag gcgcatctga cgtgacgatc atggagtcgt cgctcgctac
     5041 atcccggtcg ccgctggggc ttatcgccgg tgagggcgaa ttccccaaat tggtggcccg
     5101 cggcgcccgt gctgccggcc gcccggtcgc catcgtcgcc ttgcgcggag ccgccgacgc
     5161 cgagttgcgc gatctggccg agcgcttttg ctggcgcggg ttcgtccaag tcgggcgctg
     5221 gattcgtgcc ttgcgccgcg ccggctgccg cgaagttatc atggccggcc gcgtgcgtaa
     5281 gacgcagatg ttcgccgggc cacgctggct gcaatacttg cagtacctgc ccgacctcac
     5341 cagcatacgc ttgtggtact tccacgcccc cgacaagcgc aacgacagtc tgctcgcggc
     5401 ggtcgcggag gagatgcaac gtaagggtct gactttgatc gattcaacca cctactgccg
     5461 ccaggccctg gcgccgcttg gcgtactgac gccgttccca ccaccggccg ccgtgctgga
     5521 ggcggccgac ttcgcctggc ccatagccaa ggagatcgcg cggctggata tcggccagtc
     5581 ggtcgcggtc aaggaacgtg agatcattgc cgttgaagcg atcgagggca ccgatcggct
     5641 gatcgagcgt gccgggcagc tttgtccaca gggcggttgg acgctcgtca aagtcgccaa
     5701 accgaatcag gacatgcgct ttgacgtacc caccatcggg ccgcgcacca tcgagaattt
     5761 gaaacgtgct cgcgccaggg ggctggt
//
LOCUS       DAOSYS010000226         5761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11699_length_5761_cov_4.850158, whole genome shotgun sequence.
ACCESSION   DAOSYS010000226 DAOSYS010000000
VERSION     DAOSYS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11699_length_5761_cov_4.850158"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLU99_12540"
     CDS             <1..450
                     /locus_tag="PLU99_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17921.1"
                     /translation="AIRRFPEVLESGGTVAIIADHHDERRGLPATFLGLPTRVSRSVG
                     LLAWRYDADVVVAGNRRVGDEFAFAIEVEDVIKHEAWRGQGDADRVIRYITDRYLGAL
                     EALVRREPAQYLWGYARWGEQLARQLTAQFSTSQTQPAPPVPTTDRT"
     gene            complement(473..628)
                     /locus_tag="PLU99_12545"
     CDS             complement(473..628)
                     /locus_tag="PLU99_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17922.1"
                     /translation="MRFKRLRVLAAGVAAFLVGVPLTERAFGQVFDIVEASIELGLAI
                     ADSARFS"
     gene            complement(718..3558)
                     /locus_tag="PLU99_12550"
     CDS             complement(718..3558)
                     /locus_tag="PLU99_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOO17923.1"
                     /translation="MTRSRLAVRTLVVLLVAAGALAVADTLPAPEKITTVAGITEYRL
                     GNGLRVLLFPDESKPTVTVNITYLVGSRHEGYGESGMAHLLEHLMFKGTPDHPQVWKE
                     LQEHGAQFNGSTSYDRTNYYETLPASPENLEFALRLEADRMVNSFIAQTDLDSEMTVV
                     RNEFEMGENNVMRVLSQRIASTAYLWHNYGKSTIGSREDIERVPIDRLQAFYRKYYQP
                     DNAVLIVVGKFEEPKALLLINEIFGRIPRPERKLEATYTVEPAQDGEREVTLRRAGDT
                     QAIGVAYHICAAGHEDMAPLQVLASILSAEETGRLYKALIATKLATSVSASAFAMCEP
                     GLLDIMVEARQDQSLDEIRAQMFAVLDNLGRQQFTAEEVERAKKSFARNFALMFNDST
                     RLATRLSGSIAQGDWRLMFLHRDRLAAVTPEDVQRVAAYYLKPSNRTVGLFIPTADAE
                     RAVVPPTPDLAALLKDYKDTQEVAQGEAFEATYENIEARTERTQLPGGMKLAMLPKQT
                     RGDRVNGVLIFRYGSEADLTGKESAADFLPPMLMRGTTQHTRRQIQDRLAELNAQVRI
                     SDGGRTGGRYVQLSRGGPGILHVSIETTYENLSAVLDLVAEILRQPSFPPDEFETLRR
                     EALAGIEEQMSDPMALASNELQRRLNPCPPTDIRYIPSMPEQVERIKAVTLDEVKSIY
                     TGLVGASYSQAGFVGDFEPAELQAQLSKLFGDWSNPKPYVRIDQKYQPVSPETVVIAT
                     PDKPNAMVTLGLPLEVRDDDPDYPALVMANVVLGANANSRLVNRIRQKEGLAYGCGSM
                     ATANSLDRLGTFLAYSICAPQNAEFTLTCALEEIDRLIKDGIPQQELDEVRTGYRQQL
                     GVMLANDGTVAAMLARDLFLNRTTEFAIAQLAKIEALTPEAVQQALAKYVRPDRLVII
                     RAGDFDKSAAAEDESASEDKDARVPSGAVSPA"
     gene            complement(3572..4789)
                     /locus_tag="PLU99_12555"
     CDS             complement(3572..4789)
                     /locus_tag="PLU99_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO17924.1"
                     /translation="MSPLTVIAESFHSLGKNKIRTGLSILGIVIGIGAVIAMVAVATG
                     AQRKVEREIAAIGDDWMTIWYVSTDRGGVRREMATPLNMTREDADAIMRECSAVRAVS
                     PTNRVGAQVISQFGNTRVGVWGVYPSFFDIRRWNCTVGRCFGEEEITMRSKVCCIGAT
                     TAKELFGSVNPVGQTIRVNRAAFEIIGLLDEKGVGSDGRDFDDIIVFPFTSFQRLIAG
                     NEPSGTLFAAAKAGTPLSVAKEQIREVLRRRRHLADHEEDTFRIMDRSLMAQANAEAT
                     RTFNVLLTSIASISLIVGGVGIMNIMLVSVTERTREIGLRMAIGASSTHILGQFLCEA
                     VVLCLIGGLIGFVAGWGVSQIVTLRWQWETEVSYWMAGIAMAFSTAVGIFFGFYPAWR
                     ASRLNPIDALRYE"
     gene            complement(4792..5496)
                     /locus_tag="PLU99_12560"
     CDS             complement(4792..5496)
                     /locus_tag="PLU99_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO17925.1"
                     /translation="MHAELIDIREAWKSYHIEEVEIPAVRGVSLVIPAGQFVAITGAS
                     GSGKSTFMHVLGCLDRLDRGQYFFEGQPIERYSRRQLAQLRNRRIGFVFQSFNLLART
                     SIVDNVALPLAYQGVRRGARRRQAAEMLERVGLGDRLKHHPNQLSGGQQQRVALARAL
                     VTRPAVVLADEPTGNLDTATSQEIMRLLQAMNRDEGVSIVLVTHEPDIAAYAQRQVVF
                     RDGQVVRDTAAGSQAA"
     gene            complement(5497..>5761)
                     /locus_tag="PLU99_12565"
     CDS             complement(5497..>5761)
                     /locus_tag="PLU99_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17926.1"
                     /translation="NTALRFDPNTTTPETDWRRPGRGKPMQPRVFRLDGSELVEVPVE
                     LGLSDGKFTEIKCDALNAGDAVVVEQINRRGVAGTPRQRMPRM"
BASE COUNT         1019 a         1874 c         1783 g         1085 t
ORIGIN      
        1 gcgatccggc gcttcccaga agttctcgaa tcgggcggca cggtagccat catcgccgac
       61 catcacgacg aacgccgcgg actgccggcc acctttttgg gcctgccgac gcgcgtcagc
      121 cgctcggtcg gtctgctggc ctggcgttat gacgcggacg tggtggtggc cgggaatcgg
      181 cgcgtgggtg atgagttcgc cttcgcgatc gaggtcgagg acgtgatcaa gcacgaggct
      241 tggcgcggcc agggcgatgc ggaccgcgtc attcgataca tcaccgaccg ctatctgggc
      301 gcgttggaag ccctggtgcg ccgcgaaccg gcgcaatacc tgtggggata cgcacgctgg
      361 ggcgagcagc tcgcccggca actaacggcg cagttctcga ccagccaaac gcaaccggcg
      421 ccgcccgttc ccacgacgga ccgtacgtag gcgaattgga tcgccgcgcg ccttagctga
      481 atctggcaga atcggcaatc gcgaggccca attcgattga cgcctcaaca atgtcgaata
      541 cctgcccgaa cgcacgttcg gtcaacggca caccgacgag aaacgcggcc acgcccgccg
      601 ccagcactcg taatctcttg aaacgcattt cgcctccttt cacacgtcat ggccgcgccc
      661 agcagccagt gatagtcatt cacattatca ctttatcacg gacccggcac gccgcggtta
      721 cgccggcgat acagctcctg atggtacgcg cgcgtcctta tcctcggatg cagactcatc
      781 ctcggctgct gcggacttat cgaagtcgcc ggcgcgaatt attaccagtc gatcgggccg
      841 cacgtacttg gccaaagcct gctgcaccgc ctcgggcgtt agcgcctcga tcttggcgag
      901 ctgggcgatc gcgaactccg tcgtgcgatt caagaacaga tcgcgtgcca gcatggcggc
      961 aacggtgccg tcgttggcca gcatgacgcc aagctgctgc cgataccccg tccgcacctc
     1021 gtcgagttcc tgctgcggaa tgccgtcttt gatcaggcgg tcaatctcct ccagcgcaca
     1081 ggtcagcgtg aattccgcat tctgcggggc gcagatgcta tacgccagga acgtgcccag
     1141 ccgatccagc gaattggcgg tcgccatcga tccgcagcca tacgccaagc cttccttctg
     1201 ccggatgcgg ttcaccaggc gcgagttcgc gttcgcgccc agcaccacgt ttgccataac
     1261 gagcgccgga taatcgggat cgtcatcgcg cacctccagc ggcaatccga gcgtcaccat
     1321 cgcgttcggt ttgtcgggcg tggcgatgac gaccgtctca ggcgacaccg gctggtactt
     1381 ctggtcgatc cgcacataag gtttaggatt gctccagtct ccgaatagct tgctgagctg
     1441 cgcctgcaac tcagccggct caaaatcacc aacaaaaccc gcctgagaat agctggcgcc
     1501 aaccagcccg gtgtagatcg acttaacctc gtcgagcgtc acggctttga tgcgttcgac
     1561 ctgctcgggc atgctgggga tgtagcggat atcggtcggc gggcaggggt tcaggcggcg
     1621 ctgcaactcg ttggacgcca gcgccatcgg atccgacatc tgctcctcga tgccggccag
     1681 cgcctcgcgt cgcagcgtct cgaactcgtc gggggggaag ctcggctgcc gcaaaatctc
     1741 ggccacgaga tccaacactg ccgacagatt ctcgtacgtg gtttcgatgg atacgtgcag
     1801 aatcccggga ccgccgcgcg acagttgcac atagcgtccg ccggttcgac cgccatcact
     1861 aatgcgcacc tgggcgttca gctcggccag ccggtcctgg atttggcggc gcgtgtgctg
     1921 tgtggtgcca cgcatcagca tcgggggcag gaaatccgcg gcgctttcct tgccggtgag
     1981 gtcggcttca gagccgtagc ggaaaatcag gacgccgttg actcggtcac cgcgcgtttg
     2041 cttgggcagc atggccagtt tcatgccgcc gggcagttga gtccgctccg tacgggcctc
     2101 gatgttctcg taggtggcct cgaaggcctc gccctgcgcg acttcctgcg tgtccttata
     2161 gtccttcaac aaagccgcga ggtcgggcgt cggcggaacg acggcccgct ccgcgtctgc
     2221 agtcgggatg aacaggccga ccgtgcggtt ggagggcttc aggtaatatg ccgccacgcg
     2281 ctgcacgtcc tcgggcgtta cggccgcaag ccggtcgcga tgcaggaaca tcagccgcca
     2341 gtcgccctgg gcgatcgagc cgctcagccg cgtggccagc cgcgtgctgt cgttgaacat
     2401 gagggcgaag ttgcgggcga aactcttctt ggcacgctcg acctcttctg cggtgaactg
     2461 ctgccggccg aggttgtcga gcaccgcgaa catctgggcg cggatttcat cgagcgactg
     2521 gtcttggcgc gcctcgacca tgatgtccag aagacccggt tcacacatgg caaaagcgct
     2581 agcgctcaca ctggtcgcaa gcttggttgc aatgagcgct ttgtagagcc ggcccgtctc
     2641 ttccgcggag agaatgctgg ccagcacctg gagcggggcc atgtcctcgt gtccggcggc
     2701 gcagatgtgg taagccacgc cgatggcctg cgtatcgccc gcccggcgca gcgtcacctc
     2761 gcgctcgccg tcctgcgccg gttcaacggt gtaagtcgcc tcgagcttgc gctccggccg
     2821 cgggatgcgg ccaaaaatct cgttgatcag cagcagcgct ttgggctctt caaacttgcc
     2881 gacgacgatg agcacggcgt tgtcgggctg gtagtacttg cggtagaaag cctggaggcg
     2941 gtcgatcggc acgcgctcaa tgtcctcgcg cgagccgatg gttgacttgc cgtagttgtg
     3001 ccacagatac gcggtggaag cgatgcgctg cgacaacacg cgcatcacgt tgttctcgcc
     3061 catttcgaat tcgttgcgca cgacggtcat ttcggagtcg aggtccgtct gcgcgataaa
     3121 gctgttgacc atgcggtccg cttcgagcct gagggcgaac tcgaggttct caggactggc
     3181 gggcagcgtc tcgtagtagt tcgtccggtc gtagctggtg ctgccgttga actgcgcacc
     3241 atgctcctgc agctccttcc agacctgcgg gtggtcgggg gtgcccttga acatcaggtg
     3301 ctcgagcagg tgggccatgc cgctttcgcc gtagccctcg tgccgcgaac cgaccaggta
     3361 cgtgatattg acggtcaccg tcggctttga ttcatccgga aacagcagca cgcgcaggcc
     3421 gttgccgagg cggtactcgg tgatgccggc gacggtggtg atcttctccg gggcgggcaa
     3481 agtgtcggcc acggccaacg cgcccgcagc aaccaacagc acgaccaatg tccgcacagc
     3541 caatctactg cgtgtcatgg agaatgtgcc ctcactcgta gcgcagcgcg tcgatcgggt
     3601 tgagccggct ggcgcgccac gctgggtaaa agccgaagaa aatgccgacc gcagtggaga
     3661 aagccatcgc gatgccggcc atccagtacg agacttccgt ttcccattgc caccgtagcg
     3721 tgacaatctg cgatacgccc catccggcca cgaacccgat cagcccgccg atcaggcaca
     3781 gcacgaccgc ctcgcacaaa aattgtccga gtatgtgtgt cgagctggcg ccgatcgcca
     3841 tgcgcaggcc gatctcgcgg gtgcgctccg tcaccgagac gagcatgatg ttcatgatcc
     3901 ccacgccgcc cacgatcagc gagatcgagg cgatggaggt cagcagcacg ttgaacgtcc
     3961 gcgtcgcttc agcattggcc tgggccatca gcgagcggtc catgatgcgg aacgtgtctt
     4021 cctcatgatc ggccaggtgc cgccggcggc gcaacacttc ccgaatttgc tccttggcga
     4081 cggacagcgg cgttccggct ttcgcggcgg cgaaaagcgt gcccgacggc tcattgcccg
     4141 cgatgagccg ctgaaacgac gtaaacggga agacgatgat gtcgtcgaaa tcgcgcccgt
     4201 cgctgccgac gcctttctcg tccagcaagc caatgatctc aaacgcggcc cggttcacgc
     4261 gaatggtctg cccgacggga ttgaccgacc cgaataactc cttggcggtc gtagccccga
     4321 tacagcagac cttcgagcgc atcgtaattt cttcttcgcc gaagcagcgc ccgaccgtac
     4381 agttccagcg gcgtatatcg aagaagcttg ggtagacgcc ccacacaccc acgcgcgtgt
     4441 tgccgaattg cgagatcacc tgggcgccga cgcgattggt gggcgagact gcccgcacgg
     4501 cggaacattc gcgcataatc gcgtccgcat cctcgcgcgt catgttcagc ggcgtggcca
     4561 tttcgcgccg cacgccgccg cgatcggtgc tcacgtacca gatcgtcatc cagtcatcgc
     4621 cgatggccgc aatttcgcgc tccactttgc gttgggcgcc ggtcgccact gcgaccatcg
     4681 cgatcaccgc cccgatgccg atcacaatgc ccagaatcga aagcccggtg cgaatcttgt
     4741 tcttccccag gctgtgaaac gattcggcga tgaccgttag cggactcatg gctatgctgc
     4801 ctggctcccg gcggccgtgt cgcgcactac ctggccgtcc cggaagacga cttgccgctg
     4861 ggcataagcg gcgatgtccg gttcgtgcgt gaccagcacg atgctgacgc cctcatcgcg
     4921 attcatggcc tgcaacagcc gcatgatctc ctggctcgta gcggtgtcca ggttgccggt
     4981 gggctcatcg gccaggacga ccgcgggacg cgtgaccagc gcccgggcca acgccacacg
     5041 ttgctgctgt ccaccggaaa gctggttggg atgatgcttc agccggtcgc ccaaaccgac
     5101 gcgttcgagc atttcggccg cctggcggcg ccgagcgccg cggcgcacgc cctggtaagc
     5161 cagcggcagc gcgacgttat caacgatgct ggttcgcgcc aggagattga agctctggaa
     5221 gacgaacccg atgcgccggt tgcgcagttg agccaactgg cggcgcgagt agcgctcgat
     5281 cggttggcct tcgaagaagt actggccgcg gtcgaggcgg tcgagacatc ccaacacgtg
     5341 catgaacgtc gatttccccg aaccgctggc gccggtaatc gcgacgaact gaccggccgg
     5401 aataaccagc gaaacgccgc gcaccgcggg tatttcgacc tcttcgatgt gataggactt
     5461 ccaggcctcg cgaatgtcga tgagttccgc gtgcatttac atcctgggca tgcgttgccg
     5521 cggcgttccc gccacgccgc ggcggttgat ctgctcgacc acgaccgcgt cgccggcgtt
     5581 cagggcatcg cacttgatct ccgtgaattt gccgtcgctc agccccagct ccaccggcac
     5641 ttccaccagt tcgctcccat ccaggcgaaa gacgcgtggc tgcattggtt tgccccgccc
     5701 gggccttctc cagtctgttt ccggcgtcgt cgtattcgga tcgaatcgca gggcggtatt
     5761 g
//
LOCUS       DAOSYS010000227         5735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11759_length_5735_cov_5.900528, whole genome shotgun sequence.
ACCESSION   DAOSYS010000227 DAOSYS010000000
VERSION     DAOSYS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11759_length_5735_cov_5.900528"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="guaA"
                     /locus_tag="PLU99_12570"
     CDS             <1..1040
                     /gene="guaA"
                     /locus_tag="PLU99_12570"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOO17927.1"
                     /translation="VTHTPQGSQIIRNFLYDVCGCAGDWNVENVIETSVDAIRRQVGL
                     SDRVICGLSGGVDSSVTAALIHRAIGDRLACIFVDNGLLRRREAELVDATFRGHFGIE
                     LRTAPAAERFLSRLRGVLDPQEKRRIIGHEFIAVFKDEAQRIAGARFLAQGTIYPDVI
                     ESGHGPTGHAAVIKSHHNVGALPKELGFELVEPLRQLFKDEVRQVGLALGLPEDLIWR
                     HPFPGPGLAVRIAGEVTPERLDLLRSADEILLEEITAADWYHKIAQAFAVLVPVASVG
                     VMGDGRSYEGQNLIVLRMVETRDFMTADWVHIDYDVLARISNRIINEVRGVNRVAYDV
                     SSKPPATIEWQ"
     gene            1097..2140
                     /locus_tag="PLU99_12575"
     CDS             1097..2140
                     /locus_tag="PLU99_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO17928.1"
                     /translation="MNTTEPLRFGICGLGFMGRTYFRNLSDHPAAQVVAVCDKNEDAR
                     TGRWRATGNLAAGAAEHSDLSGIAQYARPEELLADPRVQAVAITLPTPLHAECTVEAL
                     AAGKHVICEKPMAPTLADCDRMIAAARQSGRTLMIGQCIRFWPQYEKIKALVDEGLIG
                     AVRFAKLARLSSAPTYSDGNWLLNGAASGGALLDLHVHDVDFAQYLLGLPATITAVGC
                     RGVSGQIDHSFATYSYADGRYALLEVSWAYHTPWPFEMAITVCGERGTLTWSSRSGTD
                     VLHYAGREEVQRIACENEDGWKRELDYFVQCVRAGRPVQRCLPESSRTSIELFLLEQR
                     AIRENAPVAVSGD"
     gene            2327..4201
                     /locus_tag="PLU99_12580"
     CDS             2327..4201
                     /locus_tag="PLU99_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO17929.1"
                     /translation="MKTSSRAAQTRKVPKPAQLRTRQSSPLPAWAAAIVLMAMAVMAY
                     LPAMRAGFIWDDDQYVHNNAAVRAPDGLGRIWFDFTASPQYYPLVYSSYWLEYRLWGD
                     NPAGYHVVNILLHAGVAILLWRFLLRLGVPAAWVVAAIFAVHPVNVESVAWITERKNV
                     LSGIFYFAAALAYLRFADEPQQPAADRGRRAWYAAALALFVAALLSKTVTCTLPAVLL
                     LVLWWKRSRIAWQTALPLLPFFVLGIGQGLFTSWFEKHYTGALGEQWNMTLLERCLVA
                     GRVLWFYVGKLLWPADLTFIYPRWEIDVRAWWQYVFPLAALAAPFVLFLMRRRIGWGP
                     LVAALCFGGTLFPVLGFFNVYFMQYSFVADHWVYIAGIAVILLACAAVNRLADRAPAG
                     IGAAAPFLAAAAIAVLGFLTFQQCRIYSDLEGLWRDVLQKNPGAWMAHNNLGNLLKDT
                     GRLDEARAAYEAALAVRPDFAMAHSNLGIVHKRQNRLDDAIRAYRRALECDPTYAGAY
                     FNLALAFEQRGDLDQAAAAFRSYLTLRPNHAEAHLNLGRILMIRRELAPAAESLEAAL
                     ALRPDLADAHLFLAQVRGDQGRLDEAIMHCSRVLEMNPRDADARTLLAALQAARNHRP
                     "
     gene            complement(4358..4816)
                     /locus_tag="PLU99_12585"
     CDS             complement(4358..4816)
                     /locus_tag="PLU99_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOO17930.1"
                     /translation="MLARLPRHIAERELNVIGRMLNLGGDSLKVKEVPDSHGPGNVVV
                     IEVRAAHLTEVFTAFGQRGVRAEDVAAEAARQAQQYLAADVPVGEHLADQLLLPLALA
                     GGGAFRTTALSLHAQTNMEVVRRFLDVDFQIERDLAGGIVLRVNRLSGKA"
     gene            complement(4785..5135)
                     /locus_tag="PLU99_12590"
     CDS             complement(4785..5135)
                     /locus_tag="PLU99_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO17931.1"
                     /translation="MIVVKLREAMEAYRRRTGRRITYEELAQRTGVAHSTLRSIGSRL
                     GYTPTFANLEKLCRALQVPLHDMLEMIDDPPKMKRAARQKSAGPPRSARAPARRNPEH
                     YGDRHAGPFAAAYR"
BASE COUNT          972 a         1975 c         1741 g         1047 t
ORIGIN      
        1 aggtgacgca taccccccag ggctcgcaga tcatccgcaa cttcctctac gacgtatgcg
       61 gctgcgccgg cgactggaac gtcgagaacg tcatcgagac atcagtcgat gccatccgcc
      121 gccaggtcgg gctgagcgac cgcgtcatct gcggcctttc aggcggcgta gacagcagcg
      181 tgaccgccgc cctgatccat cgcgccatcg gcgaccgcct ggcctgcatc ttcgtcgata
      241 acggacttct gcggcgccgc gaggccgagc tggtcgacgc caccttccgc gggcacttcg
      301 gaatcgagct gcgcaccgca ccggccgccg agcgcttcct ttcgcgcctc cgtggcgtgc
      361 tcgacccgca ggaaaagcgg cgcatcatcg gccacgagtt catcgcggtc ttcaaggacg
      421 aggcccagcg catcgccggc gcccgcttcc tcgcccaggg gaccatctac ccggacgtca
      481 tcgaatccgg ccacggtccg accggccacg ccgccgtcat taaatcgcat cacaatgtcg
      541 gcgcgctgcc caaggagctc gggttcgagc tcgtcgagcc cttgcggcag ctcttcaagg
      601 acgaagtccg gcaagtgggc ctggcgctgg gcctgccgga agatctgatc tggcgccacc
      661 cattcccggg ccccggcctg gccgtgcgaa ttgctggcga agtcacgccc gagcgcctgg
      721 acctgctccg ttcggcggac gaaatcctgc tggaagaaat caccgccgcc gactggtatc
      781 acaaaatcgc ccaggccttc gcggtactcg tgcccgtggc cagcgtcggc gtaatgggcg
      841 acggccgctc gtacgagggg cagaatctca tcgtgctgcg catggtggag acgcgcgact
      901 tcatgacggc cgactgggtt catattgact acgacgtgct ggcccgcatc agcaaccgca
      961 tcatcaacga ggtgcgcggc gtcaatcgcg tcgcttacga cgtatccagc aagccgcccg
     1021 ccaccatcga gtggcagtga gccagggagg cagccaatgc cggcaaccca ggtgagcatc
     1081 agcaggtgag catcaaatga atacaaccga gccgctgcgc ttcggaatct gcggcctggg
     1141 attcatgggc cgcacctact tccgcaatct gagcgaccac ccggccgcgc aagtcgtagc
     1201 agtctgcgac aaaaatgaag atgctcgaac cggccgctgg cgcgccaccg gaaatcttgc
     1261 cgccggcgcc gcggaacatt ccgatctcag cggcattgcc caatacgccc gccccgagga
     1321 attgctggcc gatccgcgcg tgcaggcggt cgccattacg ctgcccacgc cgctgcacgc
     1381 cgaatgcacc gtcgaagcgc tcgcggccgg aaagcatgtc atctgcgaga agcccatggc
     1441 ccccacgctg gcggactgcg accgcatgat cgcagccgct cgccagtccg gccgcacgct
     1501 gatgatcggc cagtgcatcc gcttctggcc gcaatatgag aagatcaaag ctctcgtaga
     1561 tgaggggctg atcggcgccg tgcgcttcgc caaactggcg cggctaagca gcgctcccac
     1621 ctactcggac ggcaactggc ttttgaacgg cgcagccagc ggcggcgcct tgctcgacct
     1681 gcacgtccac gatgtcgact ttgcccaata cctgctcggc ctgcccgcca caattactgc
     1741 cgtgggctgc cgcggcgtct ccggccagat cgaccactcg ttcgccacct acagctatgc
     1801 cgacgggcgc tatgcactcc tggaggtgag ttgggcctat cacactccgt ggcccttcga
     1861 aatggcgatc acggtatgcg gcgagcgcgg cacactgacg tggagctcgc gctccggcac
     1921 ggacgttctc cattacgccg gccgcgagga agtgcagcgc atcgcgtgcg aaaacgagga
     1981 tggctggaag cgcgagctgg attatttcgt ccagtgcgtt cgagccggcc gccccgtgca
     2041 gcgctgcctg ccggagtcat cgcgcaccag cattgaactc ttcctgctcg agcagcgcgc
     2101 catccgcgag aacgcgcccg tcgcggtcag cggcgactga acgtcggcgt cgcaactttc
     2161 cgcagcccga aaacgcattt ttataggccg ctttggccgt ggccctgctg ccgcgccggc
     2221 ttgcggcgtg atggcctcct cgccggcgcg cttctccaca tttgtcattt cgcactttcc
     2281 atttagcatt acgctcgccg gcacgagccg ccggccgcga gacgccatga agaccagtag
     2341 cagagccgcg cagacgcgca aggttcctaa accagctcaa ctccgcacgc gccagagttc
     2401 gccactcccc gcgtgggcag cggccatagt gctgatggcc atggcggtga tggcctacct
     2461 gcctgccatg cgcgccggct tcatctggga cgacgaccag tatgtccata acaatgccgc
     2521 cgtacgtgcg cctgacggac tcgggcgaat ctggttcgac ttcaccgcct cgccacagta
     2581 ttacccgctc gtctacagca gctattggct cgaataccgc ctctggggcg acaaccccgc
     2641 cggttatcac gtggtgaata ttcttcttca cgcgggcgtg gcgatcctgc tgtggcgctt
     2701 cctgctccgg cttggcgtac cggcggcctg ggtcgtagcc gcaatctttg ccgtgcaccc
     2761 ggttaacgtc gaatccgtcg cctggatcac cgaacgcaaa aacgtcctct cgggcatctt
     2821 ctacttcgcc gccgcactcg cctatttgcg gttcgccgac gagccgcagc aacctgccgc
     2881 cgaccgcggg cgccgggctt ggtatgcggc cgccttggcg ctcttcgtgg ccgccctgct
     2941 cagcaagacc gtcacctgca cgcttccggc cgtactcctg ctcgtactgt ggtggaagcg
     3001 gagtcgaata gcctggcaga ccgcgctgcc actgctcccg ttcttcgtgc tgggcatcgg
     3061 gcagggcctg ttcacaagct ggttcgaaaa gcactacacc ggcgcgctcg gcgagcagtg
     3121 gaacatgacg ctgctggaac gctgtctcgt ggccggacgc gtgctgtggt tctatgtcgg
     3181 caaattgctc tggccggccg acctgacctt catttacccg cgctgggaaa tagacgtccg
     3241 cgcctggtgg cagtacgtct tcccactggc cgcgcttgcc gccccattcg tgcttttcct
     3301 gatgcgccgc cgcattggct ggggaccgct cgtggccgca ctgtgttttg gcggcacgct
     3361 cttcccggtg cttgggttct ttaacgtcta cttcatgcag tactcgttcg tggccgacca
     3421 ctgggtctat atcgccggca tcgccgtcat attgctggcc tgcgccgccg ttaaccgcct
     3481 ggcggaccgc gcgcctgccg gcatcggtgc ggccgctccc ttcctggcgg cggccgcgat
     3541 cgccgttcta ggatttctca cgtttcaaca gtgccgtatt tactccgatc tcgagggcct
     3601 gtggcgcgac gtgctgcaga agaaccccgg cgcctggatg gcccacaaca acctgggcaa
     3661 tctgctgaag gataccggcc gcctggacga ggcccgcgcg gcgtacgagg ccgcccttgc
     3721 ggtccggccc gatttcgcaa tggcgcacag caacctgggc attgtgcata aacgccagaa
     3781 ccgcctggac gacgccatcc gcgcctaccg ccgcgctctc gaatgcgatc caacctacgc
     3841 cggggcatat ttcaatctgg ccctggcctt cgagcagcgc ggtgacctgg accaggccgc
     3901 ggccgcgttc cgcagctatc tcaccctccg gcccaaccac gccgaggcgc atctcaacct
     3961 gggtcgtatc ctgatgatcc gccgcgaact cgcgccggcc gccgaaagcc tcgaggcggc
     4021 cctggccctg cggccggacc tggcggacgc gcacctgttt ctggcacaag tccgcggcga
     4081 tcagggccgt ctggatgagg ccatcatgca ctgctcgcgc gtgctcgaga tgaatccccg
     4141 cgacgccgac gcccgtactt tactcgcggc actccaagcg gcgcgcaatc atcgcccata
     4201 atgcaattcg cctttgtccg agccgagccg gggcgtgtcg ccccggactg atgccagccc
     4261 gctgcaattc gcccttgtgc gagcagagcc ggggcgtgcc gccccggaga ataccaacca
     4321 acccgcgttt tctgaggcga acccttctac cgacggatca tgccttccca ctcagcctat
     4381 tcacgcgcag aacaatgccg ccggccaagt cgcgctcgat ttggaagtcg acatcgagaa
     4441 accggcggac gacctccatg ttcgtctggg cgtgcagcga aagtgccgtc gtccggaacg
     4501 ctccgccgcc ggccagcgcc agcggcagca gaagttggtc agccaggtgc tcgcccaccg
     4561 gaacatctgc ggcgagatac tgttgagctt gtcgggcagc ctcagcggcg acatcctcgg
     4621 cgcgcacccc gcgctgcccg aacgcggtga aaacctctgt caaatgcgcc gcacgaacct
     4681 cgatcacgac cacattgccg ggaccgtgcg aatccggcac ttcttttacc ttcagactgt
     4741 caccgccgag gttgagcatc cgcccgatga cattcaattc acgctcagcg atatgccgcg
     4801 gcaaacgggc cagcatggcg gtcgccgtaa tgctccggat ttcgccgcgc gggggcccgg
     4861 gcgctgcgtg gtggcccggc gctcttctgc ctggcggccc gcttcatctt cggcggatcg
     4921 tcgatcatct ccagcatgtc gtgcagcggg acctgcaagg cgcggcatag cttctcgagg
     4981 ttcgcgaaag tgggcgtata ccccaggcgg ctgcctatgg accgcagcgt ggagtgagcc
     5041 actcccgttc gttgcgccag ctcctcatac gtgatgcgcc gccccgtgcg ccggcggtag
     5101 gcctccattg cttcgcgcag cttaacgacg atcatgcgtg cctttgaaca gtctctttgt
     5161 gtcgccaaaa agcactttac gcttgacaac cactaattcg catggcatac tacttatcgg
     5221 attgcttatc cgcagtccgc gcacatacgc ggcgtcctgc gagggacgtt gagagtattg
     5281 gaatgtctta gcagccagtc gtcaaaccgg cgggagcgcc tgcgcgcgct cggcccgggt
     5341 gcgagaaagg atcctcgcac cagcctgcat tggcgcagtg cttggtatca cagccaatac
     5401 ggtgcacgcg cacatggcgg gcattcactc gaagctcgac acttgcgacc ggccgggaat
     5461 catcatccga gtgtgtggca tctgcctgga gcgccgcggt aggggctgcg ggcggcgcgc
     5521 cggaagctga agccataagt ggcgtgcgga cctgaagata gcgttcgcga cctagcctgt
     5581 agaggctagc ctgtacaggc taattgacgt ctcagtagtg attggcgtaa tgttgtcgtc
     5641 aacgtgcagc gggcggacgt catgcgtcgc ccgctggcgg caggggctcg ccggtgtggc
     5701 gagaccgact tcgttccaga gacgcctgcc cgcgg
//
LOCUS       DAOSYS010000228         5723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11799_length_5723_cov_6.137791, whole genome shotgun sequence.
ACCESSION   DAOSYS010000228 DAOSYS010000000
VERSION     DAOSYS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11799_length_5723_cov_6.137791"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PLU99_12595"
     CDS             <1..339
                     /locus_tag="PLU99_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17932.1"
                     /translation="LDAIIQTPADLSYVGFVPAEPFDPVVSPDGDGFAGLLFPPDVVH
                     GEDVLLGTLTFAGTGSWVDLGVTPGDLTEGFALASVGGFAAATFTGAYIPEPAALVLL
                     ALGALALRRR"
     gene            complement(523..1674)
                     /locus_tag="PLU99_12600"
     CDS             complement(523..1674)
                     /locus_tag="PLU99_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOO17933.1"
                     /translation="MATVHIDRLLETCIRVDASDIHLHVGRPPVLRMDGGLRSLETKV
                     LEPEDTVALMKSVTPERNQQELQEEGGTDFGFAFGDKGRFRVSVFRQKGNISMVLRLI
                     PSRILTFEQIGLPPIVKALCRRPRGLFLVTGPTGSGKTTTLATMIDYINRNFDRHIIT
                     IEDPIEYYHKHHKSCISQREVGVDVPSFAEALRRSLRMDPDVILVGEMRDLETMEAAI
                     RAAETGHLVFATLHTTGAQGTINRIIDAFPVNQQEQIRVQLSINVLAVLSQTLLPKMG
                     ASGRVAGYEFLVCTPAIQNLIRENKTYRIDSAIQTGKKYGMQLLDDHLWKLYDEGICS
                     ADECLERARQPGDFQAKIDAKARGQAGALGGKAGGYESQLDRDSKEEKK"
     gene            complement(1803..3515)
                     /locus_tag="PLU99_12605"
     CDS             complement(1803..3515)
                     /locus_tag="PLU99_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO17934.1"
                     /translation="MAQRRKRLGEILVSWNLLSPNALADALAYATEHKKRIGEALVEL
                     ELVKEDDVTKALATQFDLEYVDLDKNVGVTAALERVPPKLIGTYKFLPLSVENDRMKV
                     IVSDPLDLELLDLLRFRLGMEIECALAPLSKIERYINTFLTDVRSSVDEAIEEIDRDA
                     KREAEGKKAAIDVDADAPVIKLVNLIIDEAVRGRASDIHVEPMADRVRVRYRIDGVCI
                     VRDNIPKSMQAAVTARLKIMSGIDIAEKRLPQDGRIKKKIGSQLIDFRVSSLPAYHGE
                     SIVLRILRPDSVRVGITGLGFEQDSYEKFQNIINAPNGIFLVTGPTGSGKTTTLYAAL
                     QELNRPDRKIITAEDPVEYNFTGMNQCQVKEEIGLTFARILRSMLRQAPNIILVGEIR
                     DREVGEVAVQAALTGHLVFSTLHTNDAPSAIARLIDMGIKPFLVASSIQAIMAQRLIR
                     VICKECKEIDPAPDRFVLKILDIKDEDLEGRHLYRGRGCPRCGNVGYRGRQGIFELLE
                     MNNELRELAFNRAPTTQLRKAARAAGMRNLLEDGKLKIMRGVTTAAEVARIAQAEGVV
                     RDEE"
     gene            complement(3981..4712)
                     /locus_tag="PLU99_12610"
     CDS             complement(3981..4712)
                     /locus_tag="PLU99_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17935.1"
                     /translation="MTFASEHWGVDNLFNIREANPDVECGQWQFELNGLWFSQERRDD
                     RVMLWQTLKYGITDDLYAELEVHEPYICESSRDSGAGDSVFRVFWRVLRETDCCPALA
                     FDAAVRAPSGCGSSGVDGTARGMVTKTLGDCFRVHFNGFVQTANGHIGRERFDRRHFQ
                     WGAGPGVDYRLSENTVVLANYLHRVNEEYGESNQQIVEVGLIHNIPTDGNCQHAIKVG
                     GDVTVDGRDSTPHLGAKLQYQVTWK"
     gene            4730..4933
                     /locus_tag="PLU99_12615"
     CDS             4730..4933
                     /locus_tag="PLU99_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17936.1"
                     /translation="MGRAPARRQEAEAAAGVARALVRGPGPGLEHQEPEPAAEAAPEG
                     ACPVVVDWRMPKSDTELGRGILP"
BASE COUNT         1013 a         1739 c         1845 g         1126 t
ORIGIN      
        1 ttggatgcca tcatccaaac gccggccgac ctgtcgtatg ttggcttcgt gcccgcggag
       61 ccgttcgatc cggtcgtttc accggacggc gatggcttcg ccggcctgct attcccgccg
      121 gacgtggtgc acggcgagga cgtgctgcta ggcacgctga cgttcgccgg caccggtagc
      181 tgggtcgatc tgggcgttac gcccggcgac ctgactgaag ggttcgccct ggccagcgtc
      241 ggcggctttg ccgcagcgac gttcaccggc gcgtacatcc ccgagccggc ggcgctggtt
      301 ctgctggcgt tgggtgcttt ggccctgcgt cggcgctaag ccgctcgaat tgtccaccgc
      361 ggtgttttga cgccgcgcat ggcatgagag tatagacagg accgcgcttc ggtatgaccg
      421 aagcgcggtc ctgcgttaat tcgtagttca aaggacacgt tagagcgccc gttcaagggc
      481 tccccaccaa ttcagtcgct tgacggggcg gagaccagcc ggctacttct tttcttcctt
      541 ggaatcgcgg tcgagctgcg attcgtagcc gccggccttg ccacccagcg cgccggcctg
      601 gccacgagct ttggcgtcaa tcttggcctg gaagtcaccc ggctggcggg cgcgttcgag
      661 acactcgtcg gccgaacaga tgccctcgtc gtacagcttc cacaagtggt cgtcgaggag
      721 ctgcatgccg tacttcttgc cggtctggat ggccgagtcg atgcggtagg tcttattctc
      781 acggatcagg ttctggatgg cgggcgtaca caccaggaac tcatagccgg ccacacggcc
      841 cgaggcgccc atcttcggca gcagggtctg cgagagcacc gcgagcacgt taatgctgag
      901 ctggacgcgg atctgctcct gctggttaac cgggaaggcg tcgatgatac ggttaatcgt
      961 gccctgggcg ccggtggtgt gcagcgtggc gaaaaccagg tgccccgttt cggcggcgcg
     1021 aatggcggct tccatggttt ccaggtcgcg catttcgccg accaggatga cgtccgggtc
     1081 catgcgcagc gagcgccgca gagcctcggc aaagctgggc acgtccaccc ccacctcgcg
     1141 ctgcgagatg cacgacttgt ggtgcttatg gtaatactcg atcgggtcct cgatcgtgat
     1201 gatgtgccga tcgaagttgc ggttgatgta gtcgatcatc gtcgccagcg tggtggtttt
     1261 gcccgagccg gtcggcccgg ttaccaggaa cagcccgcgc gggcggcggc acaaagcttt
     1321 tacgatgggc ggcaggccga tctgctcgaa cgtcagaatg cgtgacggga tcagccgcag
     1381 caccattgaa atgttgccct tctgccggaa aacggagacg cggaaacgcc ccttgtcacc
     1441 gaaggcgaag ccgaagtccg tgccgccctc ttcctgcagc tcctgctgat tgcgctcggg
     1501 ggtcacggat ttcatcagcg caaccgtatc ctcgggttcg agcaccttgg tctcgaggct
     1561 gcgcaaaccg ccgtccatgc gcaacaccgg cgggcgcccg acgtgcaggt gaatatccga
     1621 ggcatccacg cggatgcagg tctcaagcag acggtcaata tgaacggtgg ccatgctagt
     1681 cctttggact gttagtcctt taggcggtta gtcattcagg ccgctagcca ttcgcaacgg
     1741 ggctctctcg tgcgcggagc atccctggca gcctgacagc ccaacagcct aaacgccgat
     1801 cttcattcct cgtctcgcac cacgccttcg gcctgtgcga tgcgcgcaac ttcggccgcc
     1861 gtggtcacgc cacgcatgat cttcagcttg ccgtcctcca gcaagttgcg catgccggcg
     1921 gcccgggcgg ctttgcgcaa ctgcgtcgtc ggggcgcggt tgaaagccag ctcgcgcagt
     1981 tcattgttca tttccagcaa ttcgaagatg ccctggcggc cgcggtagcc gacatttccg
     2041 cagcgcgggc agccccgccc gcggtacagg tgacgccctt ccaggtcctc gtccttgatg
     2101 tccagtatct tcagtacgaa gcggtccggc gcggggtcga tttccttgca ctccttgcaa
     2161 atcacccgga tgagacgctg agccatgatc gcttgaatgc tcgacgcaac caggaagggc
     2221 ttgatgccca tgtcgatcag acgggcgatg gcgctcgggg cgtcgttcgt gtgcagcgtg
     2281 ctgaaaacca ggtggccggt cagagcggcc tgcacggcca cttcgcccac ctcgcggtca
     2341 cgaatttcgc ccaccaggat gatgttgggg gcctgccgca gcatcgaacg caggatgcgc
     2401 gcgaaagtca ggccgatctc ctccttcacc tggcattggt tcatcccggt aaagttgtac
     2461 tcgaccgggt cttcggccgt gatgatcttg cggtcggggc ggttcagttc ctgcagcgcg
     2521 gcgtaaagcg tcgtggtctt gccggagccg gtcgggccgg tcaccaggaa gatgccgttc
     2581 ggggcgttga tgatgttctg gaacttttcg tagctgtctt gctcgaaacc caggccggtg
     2641 atgcccacgc gcaccgagtc gggccgcaaa atacgcagaa ctatgctctc gccgtggtag
     2701 gcgggcaggg agctgacgcg gaagtcgatg agctgcgagc cgatcttctt cttgatgcgg
     2761 ccgtcctgcg gcagccgttt ttcggcgatg tcgatgcccg acatgatctt caggcgggcg
     2821 gtgaccgcgg cctgcatgct cttggggatg ttgtcgcgaa cgatgcagac gccgtcgatg
     2881 cgatagcgca cacgcacgcg gtcggccatg ggctccacgt ggatatccga agccctgccg
     2941 cgtaccgctt cgtcgatgat caagttgacc agcttgatga ccggggcgtc ggcatcgacg
     3001 tcgatggcgg cttttttccc ctcggcttca cgtttggcgt cgcggtcgat ttcctcgatg
     3061 gcctcgtcga cgctggagcg gacgtctgtc aggaacgtat tgatgtagcg ctcgatcttg
     3121 ctcaaggggg ccagcgcgca ctcgatttcc atgccgaggc ggaagcggag caggtcgagc
     3181 agctcgaggt ccagcgggtc ggaaacgatg accttcatgc gatcgttttc gacgctgagc
     3241 gggaggaact tgtaggtgcc gatgagcttg ggcggcacgc gttccagggc ggccgtgacg
     3301 ccgacgttct tgtcgaggtc gacgtattcg agatcgaact gcgtggccag ggccttggta
     3361 acgtcgtcct ctttgacgag ctccaactcg accaaggctt cgccgatgcg ctttttgtgt
     3421 tcggtggcat aggccagcgc gtcggccagc gcgtttggag agagcaggtt ccaactgacg
     3481 aggatttcac cgagccgctt gcgccgttgt gccatccgtt ctccctttcc cggttcaagg
     3541 gccggcgtgc cacacgagtg cgccggggac gcaccctacg cgttgcggac gcgctgtctg
     3601 cgcaggccca agcgctgaat tgttcccgca ttagagagta aaagcaagat gtgaggggcc
     3661 gagcgtgacc gggcagccag gtaatcgggc gcttcttggg cccgccgcgg cgcccgacgg
     3721 cggcctccta ctacagggat ctagtttgcc gagcgtagta agcaaggcct gataaccgcg
     3781 gcggccggtc cgtggtgggc caaagggcga gtttgcgagt tgcgttgccg gcatgctggg
     3841 acatttccgc gcccggctac gggggcttag gaaaccagag cccgtgctgt tatcggaaga
     3901 gggccgccct cgcggtgcgg ccctcgtgct atccgattcg caagttatta agagctagcg
     3961 ctcttcccag ccttgcgcgg ttacttccag gttacctggt attgcagctt ggcgccgagg
     4021 tggggcgtcg agtcgcggcc atccacggtg acgtcgccgc cgaccttgat cgcgtgctgg
     4081 caattgccgt cggttggaat gttgtgtatc aaaccgactt cgacgatctg ctgattcgac
     4141 tcgccgtatt cctcgttcac gcggtgcaga tagttcgcca gaaccacggt gttttcgctg
     4201 aggcgatagt caacgcccgg accggccccc cactggaagt ggcggcggtc gaagcgttcg
     4261 cggcctatat gaccgttggc ggtctgcacg aagccgttga agtggacgcg gaagcaatcg
     4321 cccagagttt tcgtcaccat gccgcgggcc gtgccgtcta cgccggaaga accgcagccg
     4381 ctgggggccc gcacggcggc atcgaaggcc agggccgggc agcaatcagt ttcccgcagc
     4441 acgcgccaga aaacgcggaa gacactgtcg cccgcgccgg aatctctact gctctcacag
     4501 atgtacggct cgtgcacctc gagctcggca tacagatcat ccgtgattcc gtacttgagg
     4561 gtctgccaca gcatcacgcg atcgtcgcgg cgctcctgcg agaaccacag gccgtttagc
     4621 tcgaattgcc attggccgca ctccacgtcc ggattggctt cgcggatgtt gaacagattg
     4681 tcaacgcccc aatgttcgct ggcgaacgtc atttcgggtt cggttggcgg tgggcagggc
     4741 gccggcgcgg cggcaggagg cggaggcggc ggcgggggtg gcgcgggcgc tggtgcgggg
     4801 gccggggccg gggctggagc accaggagcc ggagccggcg gcggaggcgg cgccggaggg
     4861 ggcgtgcccg gttgtggttg actggcgaat gccaaagtcg gacactgaat tgggccgggg
     4921 gatcctgccg tagcgcttga gactagcatc atcagcccca aacacgctag agcagccgca
     4981 gttagattca ctctacctcg cataagctct ctccgttttg gatgcgcagt gcgcacttga
     5041 atggcccgca ggccgaaaca ggaacttgtt agtttatcta gactcacgcc aaccgtaagt
     5101 aaatgctaaa accgtgtgcg atactacgca agtagctgcg ttcaaaaaag ttgctgttga
     5161 ttgtcaaggt ggctaaagtc cgtcagttgc ggcaggtacc gaatccgagg ccccagggca
     5221 ttccgacaca tcagaggcca catgtcggaa acgcgcggac cacccagctt tcagccaagg
     5281 caaccaccaa gacgcagcca aggcaaccgc caagacgccg ccaaggcaac cgccaagacg
     5341 ccgagagcgc caagaccgcc gagaacaagg attcacgcgt gcgagagagg aattccgtgc
     5401 tagtgcgaca aaccgtctcg cgctggtgcg acgaattcgt ctcgcacctc gttcgggcgc
     5461 aagcctgcta agccctccat ttatgcgcaa tctggtgggt tcaacccccc agataagctc
     5521 caacccccca tataagacga ggcgacgccg cctccgtggt ggagggaaac tttaagcgac
     5581 cgaggcggga aatacccgcg gcgactggcg tctgccactc gaaactggaa actcgcaact
     5641 ggaaaccctt acaattagca gcgtcgtcag gagcgactca tgaccttgca cggcgttcag
     5701 ttcgacgagg tcaaaatcgc tga
//
LOCUS       DAOSYS010000229         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11831_length_5713_cov_3.930541, whole genome shotgun sequence.
ACCESSION   DAOSYS010000229 DAOSYS010000000
VERSION     DAOSYS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11831_length_5713_cov_3.930541"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLU99_12620"
     CDS             <1..798
                     /locus_tag="PLU99_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO17937.1"
                     /translation="VQTCETSVSSDAIFCQDCDFCERACSVRNNFLVHEMSAACCDPQ
                     ATRFSIDPLACEGCGVCVHICPAGAIDFPECSCGEWFVSRTRCGPLVHARLAPAAENS
                     GKLVSLVRAQARELAQATKREVLIIDGAPGIGCPVIASLTGATAALIVTEPTVSGRHD
                     LERVAGVARHFGAPAWVCVNKWDLNPAMTEQIEAAARVCGIQIAGRVRYDQSVTAAAD
                     GVASEVRAVWDAFGAAQWISPSRPGTSAVSAAALIKGVASNTDGSAS"
     gene            795..1721
                     /locus_tag="PLU99_12625"
     CDS             795..1721
                     /locus_tag="PLU99_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOO17938.1"
                     /translation="MSDLCSAGPAAASDPEKEIEDALLHHRIRQMGRKLLVLSGKGGV
                     GKSTVAANLAVGLARAGKRVGLLDVDIHGPSIPKLLGVDQQQPEMVGGELRPVRVNHC
                     LGVMSIGLLLCDAATPVIWRGPRKFGAIRQLLKDVAWGELDYLVVDSPPGTGDEPLAI
                     AELLAGPKSCDGGCDNVQALIVTTPQDLAVADVRRCVSFCRMLNLPVAGIIENMSGFA
                     CPGCGQVIDLFKKEGGRRLAAEMGVAFLGAIPIDPCVVVGGDAGQPVVMGTGQSPAAN
                     AFSSVVQLLLKSPATSVADTAKVRLPVAGPQK"
     gene            1738..2076
                     /locus_tag="PLU99_12630"
     CDS             1738..2076
                     /locus_tag="PLU99_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO17939.1"
                     /translation="MKIAVPVENGRLCTHFGHCAEFAVYDVDPDTRSTRSKTMHVPPP
                     HEPGVLPAWLHELGANVIITGGMGQRARQLFAESAIQVVIGAPPDPVDTVVANYLAGS
                     LQTGANPCDH"
     gene            2066..2509
                     /locus_tag="PLU99_12635"
     CDS             2066..2509
                     /locus_tag="PLU99_12635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOO17940.1"
                     /translation="MTTENRQCLLERTRGRVHSRCVVCGQANGQDARLRVTVCEDGVV
                     EAALEPSGLYEGYAGILHGGVVATLLDAAMTNCLFAHGRCGLTAELCVRFRHPVQSGT
                     LLHLRARLERSSPPLFVLNAELRQAGKLRATAIGKFMDKGCTPTP"
     gene            2566..2952
                     /locus_tag="PLU99_12640"
     CDS             2566..2952
                     /locus_tag="PLU99_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17941.1"
                     /translation="MRTLLAVVFFVALSLAGCPASGIPDAPNPEAIVIFHNNSGSMCL
                     AALNWLADIASEHPALVVEEHLTYQAGERALLRRFESRYNASEGVSGNFEYLPIIFFQ
                     GHAFSGFDAEVGQELEALMAAAQKAA"
     gene            2994..3977
                     /locus_tag="PLU99_12645"
     CDS             2994..3977
                     /locus_tag="PLU99_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO17942.1"
                     /translation="MSTVRITVLVDNYVRRRGLLAEHGWACWVETPTGRLLFDTGQGL
                     ALLHNARELGIALERADAILLSHGHYDHTGGLAPALEACPAARVFAHPAALEPKYKRA
                     PDGRIRGIGAPMSFAELHTRLAGRLVATRTPTRIAPDAWLTGEVPGATDFEPGETSFF
                     LDAAGLRRDPLSDDQALVVRTADGAVVVLGCAHSGIINTLHYIGALHPGLRLAGILGG
                     MHLQGAPVERVRRTAREFAALGIGRVCPAHCTGLSALATLAAEMAGDIEPIEVGASLE
                     FAALHDVQRGAAARADQPRCGAGGPETPPGYSGGTADRAGSQSPKNSEGSE"
     gene            4096..5292
                     /locus_tag="PLU99_12650"
     CDS             4096..5292
                     /locus_tag="PLU99_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO17943.1"
                     /translation="MSDRSHTHFGSGINTRLVHTRAAADQYGAVNVPIYQSSTFAFRN
                     AAHGAALFAGRGDGYIYTRIGNPTIRALEDTVAGLEGGFGGIATSSGLGAVASIFMAL
                     LDAGAHLVSTASVYGPSRGLVEQHLARFGVESSYVDTSDPANVREAIRPNTRLVYVET
                     PSNPAMQVTDLRAVAKIAHDNRCVLVVDNTFASPYLQRPLELGADIVLHSITKFINGH
                     ADVVGGVVVTRTEELYRKIRPLMVNLGCNMDPHQAFLVIRGLKTLGLRMDRAQQSALQ
                     IARWLEAHPKVAWVRYLGLASHPQHDLVRRQMSGFGSMISFELKGGIEAGVRLMDNVR
                     LATLAVSLGGVETLIEHPASMTHAGMSREDRIAAGITDGLVRYSVGIEDVDDLIADLD
                     QALKAV"
     gene            5381..>5713
                     /locus_tag="PLU99_12655"
     CDS             5381..>5713
                     /locus_tag="PLU99_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17944.1"
                     /translation="MLGRVIPAIGLLLLGGCPCPFCDKAPADNKARLVLQLTETEDAQ
                     ALDRMVNELRARGGTAAILTDGDFAAQNCERLQALHNEGYEIMAFLRPESGTMSDLTR
                     AEQEQLITA"
BASE COUNT          990 a         1761 c         1888 g         1074 t
ORIGIN      
        1 gtgcagacct gcgagacatc ggtttcgagc gatgcgatct tctgccagga ctgcgacttc
       61 tgcgagcgcg cctgctcggt tcggaacaat ttccttgtgc acgagatgag cgccgcctgc
      121 tgcgacccgc aagctacgcg tttttcgatc gatccactgg cctgcgaggg ctgcggagtc
      181 tgcgtccaca tttgtcccgc cggcgcaatc gactttccgg agtgctcctg cggcgagtgg
      241 tttgtgtcgc ggacacgctg cggcccgctc gtacatgcgc ggctcgcgcc ggccgccgag
      301 aattccggaa aactcgtgag cctcgtgcgc gcccaggcgc gcgaactcgc ccaagcgacg
      361 aagcgcgaag tgctcatcat cgacggcgcc ccgggcatcg gctgtccggt tatcgcatca
      421 ctgaccggcg ctaccgccgc gctgatcgtg accgagccaa ccgtgagcgg gcgccacgac
      481 ctggagcgcg tcgcaggcgt ggcgcgccac ttcggcgcac ccgcatgggt ttgcgtcaat
      541 aaatgggatc tcaaccccgc gatgaccgag cagatcgaag cggccgcgcg cgtgtgcggt
      601 atccagatcg ccggtcgcgt gcgctacgac cagtccgtga ccgcggccgc tgacggcgtg
      661 gcgagcgaag tgcgcgccgt ctgggacgcc ttcggcgcgg cccaatggat ctccccctca
      721 cggccgggca cgtcggcggt ttcggccgcg gccctcatta agggtgttgc ttctaacact
      781 gatgggagtg cttcatgagt gatctttgct cggccggacc cgcggccgcg agcgaccccg
      841 aaaaggaaat tgaggacgcg ttgctgcatc accgcattcg gcagatgggc cggaagttgt
      901 tggtgctgtc gggcaaaggc ggagtgggca agagcaccgt ggcagctaat ctggcggttg
      961 ggctggcgcg cgccggtaag agagtggggc tgctggacgt cgatatccac ggcccgagca
     1021 ttccgaaatt actgggggtc gatcaacagc agccggaaat ggtcggcggt gagctgcgtc
     1081 ccgtgcgcgt caaccattgt ctcggcgtca tgtcaatcgg tctcctgctg tgcgacgcgg
     1141 ccacacccgt aatctggcgt ggacctcgca agtttggtgc gatccggcag cttctaaagg
     1201 acgtcgcctg gggcgaattg gactatttgg tggtcgattc gccgccgggc acgggagacg
     1261 agccgctggc gattgccgag ttgcttgccg ggccgaagag ctgcgacggg ggctgtgaca
     1321 atgtccaggc gctgatcgtc acgacacctc aggatctcgc ggtcgccgac gtgcggcgct
     1381 gcgtcagctt ctgccgcatg ttgaacctcc cggtggccgg cattattgag aacatgagcg
     1441 gcttcgcctg cccaggctgc ggccaggtga tcgatttgtt caagaaggag ggcgggcgaa
     1501 gactggccgc cgagatgggc gtcgcgtttt tgggggccat tccgatcgat ccgtgcgtcg
     1561 tcgtcggcgg agatgcgggg cagccggtcg tcatggggac cggtcagagc cccgcggcca
     1621 acgccttttc ttctgttgtg cagcttttgc tgaaatcgcc cgcgacgtca gtcgcggata
     1681 cggcgaaggt ccggttgccg gtcgccgggc cgcaaaaata acttggagga caaccgcatg
     1741 aagattgctg tccccgtcga aaacggacgc ctttgcacac atttcggtca ctgcgccgag
     1801 ttcgctgtct acgatgtcga cccagacacg aggagtacgc gcagcaagac catgcatgta
     1861 cctccgccgc acgagccggg cgtgttgccg gcctggctgc acgagttggg cgcgaacgtc
     1921 atcatcaccg ggggaatggg acaacgggcg cggcagctct tcgccgagag cgccattcag
     1981 gtcgtcatcg gagcgccgcc ggatcccgtc gacaccgtcg tggcgaatta cctcgccggc
     2041 agcttacaga ccggagccaa tccttgtgac cactgagaac cgccaatgcc tgctggagcg
     2101 aactcgtggt cgagtccaca gccggtgcgt cgtatgcggc caggctaacg gacaggacgc
     2161 gcgcctgcgc gttacggtct gtgaggacgg ggtagtcgag gccgctctag agccgagcgg
     2221 cctttatgaa ggctacgccg gcatcctgca cggcggcgtc gtcgccacgc tgctggacgc
     2281 cgcgatgacc aactgcctgt ttgcacacgg gcggtgcggg ctgacggccg agctgtgcgt
     2341 gcgttttcgg catccggtgc aaagtggtac attgttgcat ttaagagcgc ggttggagcg
     2401 gtcttcgccg cccctatttg tactgaacgc cgaactgaga caggccggga agttgcgcgc
     2461 cacggcgatc ggaaagttta tggataaggg ctgcacgccg acgccgtgag tagcgttcgg
     2521 cgaattggca gaggtggatc agatatacaa tccgtgtggt gagccgtgag aactctgctg
     2581 gctgtcgtct tcttcgtcgc cctgtcgctc gctggatgtc cggcgagtgg tatacctgac
     2641 gctccaaatc cggaggctat cgtcatcttc cacaacaaca gcggctcgat gtgtctggcg
     2701 gccctgaact ggctggcaga tatcgcgagc gaacatcctg cgctagtggt cgaagagcat
     2761 ctgacttacc aggcgggcga aagggcgctg ctgcggcggt tcgaatcacg gtacaacgca
     2821 agcgagggcg tatccggcaa tttcgagtat ttgccaatta tcttctttca aggacacgcc
     2881 ttttccggct tcgacgcgga ggttggccaa gaattggagg cgttgatggc agccgctcag
     2941 aaggctgctt gagcacccgg cggccgtgat ggtacgcggt tggtgagcgg tccatgagta
     3001 ctgtgcgtat caccgttctg gtcgacaact acgtgcggcg ccgaggcctg ctcgccgaac
     3061 acggctgggc ctgctgggtc gaaacgccca ccggtcgcct gttgttcgat acggggcagg
     3121 gactggcgct gctgcacaac gcgcgggagc tgggcatagc gctcgagcgg gcggacgcca
     3181 tcctgctgag ccacggtcac tacgatcaca ccggcggtct ggcacccgcg ttggaggcct
     3241 gtcccgctgc gcgcgtgttc gctcatccgg ccgctttgga gccgaaatac aagcgcgccc
     3301 cggacggccg aatccgcggg atcggcgcgc cgatgtcgtt cgcggaattg cacacgcggc
     3361 tggcagggcg gctggttgcc acgcgcacac cgacccggat tgcgcccgat gcatggctga
     3421 ccggcgaagt gcccggcgcc actgacttcg agcccggcga aaccagcttc tttctggatg
     3481 cggccggcct gcgacgcgat ccgttaagcg atgatcaggc cctggttgtg cgcacggcgg
     3541 acggggccgt cgtcgtgctg ggctgcgccc attccggcat cattaacacg ctgcactaca
     3601 tcggcgccct gcaccccggc ctgcgattgg cgggcatcct gggcggaatg cacttgcagg
     3661 gtgcgcccgt ggagcgggtg aggcgtacgg cccgtgagtt cgccgctttg ggtatcgggc
     3721 gggtttgtcc ggcacactgc actgggctgt ccgccctggc gactctggcg gcggaaatgg
     3781 caggtgatat cgagcccata gaagtaggcg cgtcgctgga gttcgcggcg ctgcacgacg
     3841 ttcagcgcgg cgccgctgcc cgggcagatc agccgcgatg cggtgctggc ggtcctgaaa
     3901 cgccgcccgg gtactctggc ggaactgccg accgcgctgg cagccaatcc ccaaaaaatt
     3961 ctgaaggttc tgaatgattt gttttgagta acggccgcgc gcatggaagt cctcgtccac
     4021 cagacgtact acattctccg cgggccataa cacacggaag gtgaaaacat ggtttctgac
     4081 gacgcgaatg caagtatgag tgatcgctcc cacacgcact tcggctcggg catcaacacg
     4141 cggctggtgc acacgcgggc cgccgccgac cagtacggcg ctgtgaatgt cccgatttac
     4201 cagtcgtcga cgttcgcctt ccgcaatgcg gcacacggcg ccgcgctctt cgccgggcgc
     4261 ggagacggtt atatctacac ccgcatcgga aacccgacta tccgtgcgct ggaagataca
     4321 gtcgccggcc tcgaaggcgg ctttggcggc atcgccacca gttccgggct gggcgccgtc
     4381 gcttcgatct ttatggccct gctggatgcc ggcgcgcact tggtcagcac cgcctcggta
     4441 tacgggccgt cgcgcgggct ggtcgaacag catctggcgc gcttcggcgt ggaatcgtca
     4501 tacgtggaca cgtccgatcc ggccaacgtg cgggaggcga ttagacccaa cacccgcctg
     4561 gtatacgtcg aaacgccgtc aaatccggcc atgcaggtca ctgacctgcg cgccgtcgcg
     4621 aagatcgcgc atgacaaccg ttgcgtgctc gtggtggaca acaccttcgc cagcccatat
     4681 ctgcagcggc cgctggagtt gggcgccgac atcgttctgc attccattac gaagttcatc
     4741 aacgggcacg ctgacgtagt cggtggggtt gtggtcacgc ggaccgaaga actgtaccgc
     4801 aaaatccggc ctctaatggt aaatctgggc tgcaacatgg acccccatca ggcctttctg
     4861 gtcattcgcg gcctgaagac tcttggcctg cgcatggacc gcgctcagca aagtgcgctg
     4921 cagattgccc gctggcttga ggctcacccc aaggtggctt gggtccgcta cctcggcttg
     4981 gcgtcgcatc cgcaacacga ccttgtccga cgccagatgt ccggcttcgg ctccatgatc
     5041 agttttgaac tgaaaggcgg tatcgaggct ggcgtgcgtt taatggacaa tgtacgcttg
     5101 gctacgttgg ctgtatcgct gggcggcgtc gagacgctga tcgagcaccc cgcgtctatg
     5161 acacatgcag gaatgtcccg cgaggatcgt atcgcggccg gcattaccga tggcctggtt
     5221 cgctactccg tgggcatcga agacgtggac gacttaatcg ccgatctcga ccaggccctg
     5281 aaagcagtct gagcaattca gtgacggccg gtgtggcgcg tgccgccgga ctgccagact
     5341 ggtcgaacga gaggtgcgcg ccggaaagga gtgcagtccc gtgctcggca gagttattcc
     5401 tgcaataggc ctcttgctgc tggggggttg cccgtgtcca ttctgtgaca aggcgcccgc
     5461 ggataacaag gcccgcctcg tcctgcagct cacggaaacc gaggacgccc aagccctcga
     5521 ccgcatggtc aacgagctgc gcgcccgcgg cggcacggcc gccattctga cggacggcga
     5581 ttttgcagcc cagaactgcg aacgcttgca ggcattgcac aacgagggct acgaaatcat
     5641 ggccttcctt cggccggagt ccggcacgat gtcagacctg acccgcgccg aacaggagca
     5701 gctcatcacg gcc
//
LOCUS       DAOSYS010000230         5695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11885_length_5695_cov_5.988830, whole genome shotgun sequence.
ACCESSION   DAOSYS010000230 DAOSYS010000000
VERSION     DAOSYS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11885_length_5695_cov_5.988830"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..278)
                     /locus_tag="PLU99_12660"
     tRNA            complement(207..278)
                     /locus_tag="PLU99_12660"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(244..246),aa:Asn,seq:gtt)
     gene            complement(682..2436)
                     /locus_tag="PLU99_12665"
     CDS             complement(682..2436)
                     /locus_tag="PLU99_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOO17945.1"
                     /translation="MILNIAVLALILAITFMHSLFGLFSGLINAMCAITAMCVALGFS
                     EAVNALAVEQGMPPSYSLAVTLMVLFVVTHLVLRLAADNLIRGNVHVPRALDVGGGAV
                     CGALIAMISVGMLVMAFLALPFGGRVAKFSRYERTENVQDDGLVKFERRSVWLKPDEF
                     TAGLFSLLSGGSLKHQHAFAEVYPSFAEWVTWTGNTVQPESATSPGHDSKSDGFGSRG
                     LAIQTWWVHKGSLDAYYRTKLPAKGVSARELEFKPLAYKPAPGKQLVGVRLKLMAAAA
                     DPHESARVHRFRPSMLRVVGVENGVPRQYVPRALGGVIAGEDKIRLVDVDNNFALSAR
                     ENETLVEAYFEVGEDFEPRFAEYRRFARAVFPKEKAAAAPADRLVGEDTSSAKSRASG
                     AFMQAVVERGTGCLSELPYALSASKLRAMAGVELKDGALKCGRIHGLVEDLTGAGGDK
                     VKQFVLPEGQGLFQLRFKARRAASLAGKVFDFVASVTNQYMAVARGGERYSLAGYYAV
                     VKRSGADYLELYLTCEEEAAAGRSMLEFKQITNTELRERDTEIGLLFFVRLGEVITGV
                     ENQKRQGVEFAPPGYKIQ"
     gene            complement(2452..4185)
                     /locus_tag="PLU99_12670"
     CDS             complement(2452..4185)
                     /locus_tag="PLU99_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO17946.1"
                     /translation="MLLAYLSTMAQFTGDSIYVSPLKLPAILVVFVLWTFLAQWVDKD
                     TVAVNTFRILWNTVVVACGVIGVVIALLVPNFLIGFPVFVAAVLTGGIVYVVHRNGLV
                     TPEHTVLTAAHFRRLREQGLKGKKQVKEVKERVRVTGANRQVVTIPDDEVEREQYRLM
                     QDLLFDAFWRRAAITDITPAGQSTRITYLIDGVPTEREGLPRQEGDAVLLYLKRIAGL
                     NLEERRKPQRGKITVALGDSKHTVVLQSAGTTAGERLRLRVVGPEGSCKVKDLGFTET
                     QLQQVRAVMGLPRGLVLLTAPKGNGLTTTVYGFVRSHDAFLQNIQILEYDREIEVENV
                     TQHLFVPKDESTFSGELQKIVRSDPDILVLPEVRDKETMQLATKAAAEKAKVYVGFNA
                     DNVFDALRKWTALVGDKGLVAKGLVMITNQRLVRVLCSECKERYKPDPGMLRKLNMPP
                     EAMLHRPPEPTYDKRGNLIVCQACQGTGYVGRTGVFEALIVDDEVRRVIRAAGSISEV
                     ESFALKRGGVGLQTPAMAKVLNGLSSIQEIVRVLKGQSARPASGSGPAGSPAGASSRP
                     APSSQAGKTPR"
     gene            complement(4277..5437)
                     /locus_tag="PLU99_12675"
     CDS             complement(4277..5437)
                     /locus_tag="PLU99_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOO17947.1"
                     /translation="MGDELEVMDQAPSGGDGQAKEPFLNKYFKATIRMKVSDLHLKAG
                     SPPRVRVRGDLRPLTGGPLTADQIRDGIFEMISPRLRQIYEEKGAVDFAYDVGPPGDA
                     DRFRVNAFQQRGKMSVAARRVSREIRNFESLKLPPSISKIAELHQGLVLLAGITGSGK
                     STTIAAMVDYINERFPVHIVTIEDPIEYLFTDKKACINQREIGIDVESFPDALKYLMR
                     EDPDVVLIGEMRDQETFSAALHAAETGHLVFGTIHASSSSQTISRLLDLFPEGSRRLV
                     RQTLEFNLQAIICQKLIPCVKPDCPLVPAVEIMISNPSIRKLIREERDNEIVDVIRAS
                     YDAGMIDFTEHLRQLVATGYIDHATAYEAAPNPDELRMALKGIRAASAGILG"
BASE COUNT         1085 a         2029 c         1576 g         1005 t
ORIGIN      
        1 atacgacgat gggccgcaag ctgagcggca ggcgctgcgg cgcgagggta cgaacgacgc
       61 gccggtacag gtggaccaaa tactggacca aaaggggtgc cgggacgggc gcggcagtgc
      121 gcgagggtgc gggcgcggtg ctggcaacaa cgcaacaaac gccggaacaa acgcggtttc
      181 cgcgcgaata gcaagagagg ggaaaactcc ccgcgtagga ctcgaaccta caacaacccg
      241 gttaacagcc gggtgctcta ccattgagct agcggggacc agaatgacgc ccgggatcaa
      301 agccccgaac atccaaagcc taccagaccg tgcgttgctg tcaagccgaa tggacggcaa
      361 cccagcagcc gggacagccg ggacacggcg gccgaagcag ccgcggtagc atggcagcct
      421 gaacggccgc accagcttgc acgcttgccg gcttgcgccg ccaggggcat cgcccgcgtt
      481 taccgggcgt tggatgcgcg gcgccaacca agccagttcg gcgcgttggc ccgcggcgcc
      541 aaccaggccc cttcgccgcg ttggcgcgcg gggccaacct tatcagaccg catccgggcg
      601 cgctactgga tgttcgggcg cggtactcga tgctcgtgtc cgctactggg cgtccgggcg
      661 cgctacagaa gttctagcgc gctactggat cttgtagccc ggcggcgcga actccacccc
      721 ctgtcgcttc tggttttcaa cgcccgtgat gacctctccc aaccgcacga agaacagcag
      781 accgatttcc gtgtcgcgct ctcgcaattc ggtgttcgtg atctgcttga attccagcat
      841 gctgcgcccg gccgccgctt cttcctcgca ggtaaggtac agctccaggt agtcagcccc
      901 tgaccgcttc accacggcgt agtaacccgc cagagaatag cgctcacccc cccgggcaac
      961 cgccatgtac tgattcgtaa cgcttgccac aaaatcgaaa actttgccgg ccagcgacgc
     1021 cgcccgccga gccttgaagc gcagttggaa cagcccctgc ccctcgggca acacaaactg
     1081 cttcaccttg tcgccgcccg cccctgtcag atcttccact aaaccgtgaa tccgcccgca
     1141 cttcagcgcg ccatccttca actctacccc cgccatagcc ctgagcttgg aggccgacaa
     1201 agcgtacggc agctcggaaa ggcagccggt gccgcgttcc acaaccgcct gcatgaatgc
     1261 cccgctggcg cggctcttcg cggacgacgt gtcctcgccc accaatctgt ccgccggcgc
     1321 cgcggccgct ttctctttcg gaaacacggc ccgcgcgaag cggcgatact ccgcaaaacg
     1381 aggctcgaaa tcttcgccca cttcgaagta ggcctccacg agcgtttcgt tctcccgcgc
     1441 cgacagcgcg aagttgttat ccacgtcgac caggcgaatc ttgtcctctc ccgcaataac
     1501 cccgcctaac gcacgaggca cgtactgtcg cggaacgccg ttctccaccc ccacgacgcg
     1561 cagcatcgat ggccggaagc ggtgaacacg cgcgctttca tgcgggtcgg cggccgccgc
     1621 catgagcttc agccgcacac cgacgagttg cttgcccggg gccggcttat acgcgagcgg
     1681 cttgaattca agctccctgg ccgacacgcc tttggcgggc agcttcgtcc ggtaatacgc
     1741 atccaaggag cccttgtgca cccaccaagt ttgaatggcc aacccccgcg acccaaaacc
     1801 atccgacttg ctatcgtgcc ccggcgaggt cgccgattcg ggctgtaccg tattccccgt
     1861 ccaagttacc cattcggcaa agctcggata gacctccgcg aaagcatgct ggtgtttgag
     1921 actccccccg ctgagcagac tgaacaagcc ggcagtaaac tcgtccggct tgagccacac
     1981 gctgcgacgc tcaaacttga ccagcccgtc gtcttgcacg ttttccgtcc gctcgtagcg
     2041 cgagaacttc gccacccgcc cgccaaacgg caacgcgagg aacgccatca ccagcattcc
     2101 cacgctgatc atggcgatca gcgctccgca aaccgccccg ccccctacgt ccaacgcccg
     2161 cggtacatgc acgttgccgc ggatcaaatt gtccgccgcg agccgcaaga ccaagtgcgt
     2221 taccacgaac aacaccatca gcgtcaccgc cagcgagtag ctcggcggca tgccctgctc
     2281 aaccgccagc gcattgaccg cctcggaaaa tcccaaagcc acacacatgg ccgtgatcgc
     2341 acacatcgca ttaatcagcc cgctgaacag gccgaagagc gagtgcatga acgtgatcgc
     2401 caaaatcagc gccaataccg cgatgttaag tatcatgcat aagctccggt cttagcgggg
     2461 ggtcttgccg gcctgggacg acggggccgg ccgcgaactg gcgcccgccg gactgcccgc
     2521 cgggccggac ccgctcgcgg gacgcgcact ctggcctttc agcacgcgca cgatttcctg
     2581 aatgctgctc agtccgttga gaactttggc catggccggc gtctgtagcc cgacgccgcc
     2641 gcgcttcagc gcgaacgact ccacctcgga gatgctgccc gccgcccgaa tcacgcggcg
     2701 cacctcgtcg tccacgatca gtgcctcaaa cacccccgtg cgccccacgt aacccgttcc
     2761 ctggcaggcc tggcagacaa tcaggttgcc gcgcttgtcg tacgtcggct ccggcggacg
     2821 gtgcagcatg gcctccggcg gcatgttcag cttgcgcagc atgcccggat cgggtttgta
     2881 gcgctccttg cactccgagc acagcacccg caccaggcgc tgattcgtaa tcatcaccaa
     2941 gcctttggcc accagcccct tgtcgcccac gagcgccgtc cacttgcgca gcgcgtcgaa
     3001 tacgttgtcc gcgttgaacc caacgtaaac cttggccttc tccgcggccg ccttggtggc
     3061 cagttgcatg gtttccttat cgcgaacttc cggcagcacc aaaatgtccg ggtcgctccg
     3121 cacgatcttc tgcaactcgc ccgaaaacgt gctctcatcc ttgggcacga ataggtgctg
     3181 cgtcacgttc tcaacttcaa tttcgcggtc gtactccaaa atctggatgt tctgcaagaa
     3241 cgcatcgtgc gagcgaacaa acccgtagac cgtcgtcgta aggccgttcc ccttcggggc
     3301 cgtcagcaga accagcccgc gcggtaatcc catcaccgcc cgcacctgct gcaattgagt
     3361 ttccgtgaaa cccaggtcct tcaccttgca ggaaccctcc ggcccgacca cgcgcagccg
     3421 taggcgttct ccggccgtcg tgccggccga ttgcagcacc accgtatgct tgctgtcccc
     3481 aagggcgacc gtgatcttgc cgcgctgtgg cttacggcgt tcttcaaggt tcaggccggc
     3541 gatgcgtttg aggtacagaa ggaccgcgtc gccctcctgg cgcggcaagc cctcgcgctc
     3601 cgtcggcacg ccgtcgatca ggtacgtgat tctcgtcgat tgtccggcgg gggtgatgtc
     3661 cgtgatagcc gcgcggcgcc aaaacgcgtc gaagagcaag tcttgcatga ggcggtactg
     3721 ctcgcgctcc acctcgtcgt ccgggatggt gaccacctgc cggttggcgc cggtcacgcg
     3781 cacccgctcc tttacttcct tgacctgctt cttgcctttg agcccctgct cgcggaggcg
     3841 tcgaaaatgg gccgccgtca aaaccgtgtg ttcgggcgtc accagcccgt tgcgatgaac
     3901 gacgtacact atccccccag tcaaaaccgc cgctacaaat accggaaacc cgatcaggaa
     3961 gttcggcacc agcagcgcga tcaccacccc gatcacgccg caagccacga ccaccgtgtt
     4021 ccacagtatt cggaacgtgt ttaccgctac tgtgtccttg tcgacccact gggccaggaa
     4081 cgtccacagc acgaaaacca ccaaaatcgc cggcagcttc agcggactga catatatgct
     4141 gtcccccgta aactgggcca ttgtagacaa gtacgccagc agcatacgtt ctcccgtcag
     4201 cacgcagcgc gccccggcca ggcgccgcct ggatgcgccg caccaggctg acaactcaca
     4261 tcaaacaact gattactcaa cctagtatgc ccgccgaggc ggcccggatg cccttcaaag
     4321 ccattcgcag ttcgtccggg ttgggggcag cctcgtacgc cgtggcgtgg tcgatgtagc
     4381 cggtcgccac cagttgccgc aggtgctcgg taaaatcgat catgcccgcg tcgtaggacg
     4441 cccggatcac gtccacgatc tcgttgtcgc gctcctcgcg aatcaacttc cggatcgacg
     4501 gattggaaat catgatttcc accgccggaa ccagcggaca gtcgggtttt acgcacggaa
     4561 tcagcttctg gcagatgatc gcctgaaggt tgaactccag cgtctgccgc accagccgcc
     4621 gcgaaccttc cgggaacagg tccagcaaac gcgaaatcgt ttgcgacgag ctcgaagcgt
     4681 gaatcgtgcc gaaaaccagg tgccccgttt cggccgcatg caacgcggcg ctgaacgtct
     4741 cctggtcgcg catttcgccg atcagcacca cgtccgggtc ctcgcgcatc aggtatttca
     4801 gcgcgtccgg gaagctctcg acgtcgatcc cgatctcgcg ctggttgatg caggccttct
     4861 tgtcggtgaa caggtactcg atcgggtcct cgatcgtcac gatgtgcacc ggaaagcgct
     4921 cgttgatgta atccaccatc gccgcgatgg tggtactctt gcccgaaccg gtgatacctg
     4981 ccagcagcac caacccctgg tgcaactcgg caatcttgga tatcgacggc ggcagcttca
     5041 ggctctcaaa gttccgaatc tcacgcgaca cacgccgcgc cgccaccgac atcttgccac
     5101 gctgctgaaa cgcattcacg cggaaacggt cggcatcccc tggcggccca acatcgtacg
     5161 cgaaatccac cgcgcccttt tcttcgtata tctggcgcaa gcgcggcgaa atcatttcga
     5221 agatgccgtc gcgaatctga tcggccgtca gcggcccgcc cgtcagcggc cgcaggtcgc
     5281 cccgcacccg cacgcgcggc ggcgaacccg ccttcaggtg caggtcagaa accttcatcc
     5341 gaatggtggc cttgaagtat ttgttcaaaa acggttcttt ggcctgacca tctcctccgc
     5401 tcggagcctg atccatcact tccagttcat cgcccatatc gaacggctcc tgctaccccg
     5461 agccggcgct ccgtccactc accggcccgg cggctcacct cgtcgtggtc gaccacgcag
     5521 tatactgccc cggccgccgc ccatctagtc agcccccgct cggccccccg ccccgtcggg
     5581 tgccatgccc aagccgcgta gcggcccggg catgccgtca ccgtttaccc cccgacgccg
     5641 caacgccgga aatcacaggg gcatgcccgc gcttacagct tgggcatggc acccg
//
LOCUS       DAOSYS010000231         5682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11923_length_5682_cov_6.465968, whole genome shotgun sequence.
ACCESSION   DAOSYS010000231 DAOSYS010000000
VERSION     DAOSYS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11923_length_5682_cov_6.465968"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..632)
                     /locus_tag="PLU99_12680"
     CDS             complement(309..632)
                     /locus_tag="PLU99_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17948.1"
                     /translation="MADTIKCACSHCGAKYRLPIEAQGRTARCKRCGEKFEVPRQESS
                     LEDTILSWLTPPEEEEADVAKPRVISMPREPVDPEAAKRARGPIRLKEESEPDAKQAR
                     TAKAT"
     gene            complement(889..2523)
                     /locus_tag="PLU99_12685"
     CDS             complement(889..2523)
                     /locus_tag="PLU99_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOO17949.1"
                     /translation="MKIVAAVFADFQDTFLGRPSQLSTRLGSRSVLEHTLDRAARVTG
                     LDGRCLFVRPRDREAARAALDESGHAPAFELLSLDDGLRRRRALLRCARKWNLHAWRG
                     SLLGSTWFDEYVEAGPVARVLKHYECDAVLCLDGHQPALDARLASGMVAQMRERADQA
                     GLIFSQAPPGLAGLVLTRDAVSQLLAQDIPVGLLLSYRPEMPRGDPITQPSCFQVDPE
                     VARTTARFAADTRLSAELLDQAFATLGEQVESGPLCAFIRERQQAHVRKLPAEVEVEL
                     TTATPLPESTLRPPGVPPRELYNIADLSRLGDQLAAYDDQLVVLGGHGDPLSHPRFAE
                     ACERLRAGVLGLAVRTPLVNLSDDCLEALFAHKVDILQVRLDANSADTYRRVHRLDCF
                     ERVIANIERIERLRRERVSAEPIVVCSLTRCAPTLPEMEEFYDHWIKRTGWAVIEGYN
                     DYCGALPEDSLLRADPPVREPCRRLSHSFVLLADGAATLCHQDYRGQLAIGDWRTKPL
                     TELWASAEIANARQAHARGELDSYPACQRCREWFRP"
     gene            complement(2520..4520)
                     /locus_tag="PLU99_12690"
     CDS             complement(2520..4520)
                     /locus_tag="PLU99_12690"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOO17950.1"
                     /translation="MMDNSVALDERPPDTAACFLKEPLGHEESPLDRLRAVYVKNLAQ
                     LYRRQQALALRLEAVPFSSCPALERTRDGRFTAQLPTAGGRAVYLHSRYRPIEEAQQF
                     VEAQSRAEDGADGARAPGAFLLCGIGLGYHLAELLRRHEVARVIVAEQDLALIKAALT
                     VVDISNELHEGRVFFLTSAEKSHVHEVLRPANIDIMLGLKVIALPYATQHRAEFHSQA
                     RSALQDFICYARLQMVTVLRNARITCKNILFNLPAYLARPGVEVLRNRAAGYPAILVA
                     AGPSLARNIDQLAGLRERAVIIAVQTVFKQLLARGIPPHFVTSLDFHEISAQFFHGVE
                     DFRDCILVAEPKAAWQVLEAYRGRTHVLHNNLCDAVLGPAAPVRGRLKAGSTVAHLSF
                     YLAEYLGCDPIILIGQDLSFSDGLYYPPGLPIEQTWAPELNRFYTVEMKQWERIVRAR
                     PILRTVTDVHGRKTYTDDQLFTYAEQFQTDFARTRARVIHACEGGMRLAGTEPMKLRE
                     AGERFCTRPLPADLFAAGGDPLSRELQVQAQEALQKRLADVGEVAEIARIMRGLLDEL
                     AERVDRPAEFNKLVPKVDELRVRLRRYEQIYGMVVGVSQVAELRRYSADRRIRAQQHE
                     DANAVRQGLARDREFVSAFIDGCEFLMQVLPQAMQRLGEGVR"
     gene            4744..5091
                     /locus_tag="PLU99_12695"
     CDS             4744..5091
                     /locus_tag="PLU99_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17951.1"
                     /translation="MTTTTCSRETVAPIQESAASSRENLAPKQNSAPTRTAEPGTTLP
                     LDVLSEPGAYVCNWSGHLLRVRPDAFPGGRPPGLNIVGDRPLTITKISDDPDVVISKA
                     KRLAAFFNLEVKF"
     gene            5379..5672
                     /locus_tag="PLU99_12700"
     CDS             5379..5672
                     /locus_tag="PLU99_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17952.1"
                     /translation="MAVEPMTCAEETGSEEGRIALGACVPFDVIHEPGAYVCNWSGHL
                     LRVRADALERAHDVPVNLVGREPLLVTKISSNPQIAVNQARSIASQLSVAVNF"
BASE COUNT         1076 a         1829 c         1777 g         1000 t
ORIGIN      
        1 acgctcttgc aaaagggcga ctttgcgccg ggtcaattgc tgtctgccga gtgcatcggg
       61 actgacggct acgacctggt cgcgcgcccc atccgccaaa cgcgataaca ctcatccgtg
      121 tgccggaagc cgtgcacacc ctcgatgctg tgataaggtg ataaggtgat agggtgataa
      181 aggatgatcc gcggaacccc tttatcactc tgccacgtta tcactcttat caccggctat
      241 caccgggtgc gtagccgggc gccatgccca agctgtaagc gcgggcatgc cctcgtttac
      301 ggctacgcct acgtggcctt cgcggtccgc gcctgtttcg cgtccggttc cgactcttcc
      361 ttcagccgga tcggaccgcg ggcgcgcttg gctgcctccg ggtcaaccgg ctcgcgcggc
      421 atgctgatga cgcgtggctt ggcgacgtcg gcttcctctt cttccggcgg ggtcagccaa
      481 ctgaggatgg tgtcctcaag agaggactcc tggcgcggca cctcgaactt ttcgccgcac
      541 cgcttacagc gcgcagtgcg gccctgcgct tcgatgggga ggcgatactt cgccccgcag
      601 tgggaacaag cacatttgat tgtgtcggcc acgacacacc tcgttccgtg gcggtcgcga
      661 actgggtaat cctgcagtcc cagcccggcg gtcgccaaac gcctgtgaaa cgaatcctaa
      721 ttgtccggct gaggatgatt cggcgtatga acgagataag tccgaaggca aggctagtta
      781 atcattatga ttcgcgcctg cctcagccat tagtatgcta acggacgaat cgatcggttt
      841 caataggttc tcgtctgatt gcactctaac cggagattta cgcgcccgct atggccggaa
      901 ccactcccga caccgctggc aggctggata actatccagc tcacctctgg cgtgggcctg
      961 gcgcgcattg gcaatttcgg cagatgccca caactccgtt agcggtttcg tccgccagtc
     1021 gccgatcgcc agttggccgc ggtaatcctg gtgacacagc gttgccgcgc cgtcggcgag
     1081 caagacgaac gaatggctta gccggcggca aggttcccgg acgggcggat cggcacggag
     1141 caaactatct tccgggagcg cgccacaata gtcgttatag ccctcgatga cggcccaacc
     1201 ggtgcgcttg atccagtggt cgtagaactc ctccatttcg ggcaaggtcg gcgcgcaacg
     1261 ggtcaagctg caaaccacta tgggctcagc acttacgcgc tcgcgccgca gccgctcgat
     1321 gcgttcgatg ttcgcgataa ctcgctcgaa gcagtccaag cggtgcaccc gccggtacgt
     1381 gtcggcggag ttggcatcta atcggacctg cagaatgtcg actttatggg cgaacagagc
     1441 ttcgaggcaa tcatccgata agttgacgag cggcgtgcgc accgccagcc cgagaactcc
     1501 ggcccgtaac cgctcacagg cctctgcgaa ccggggatgg gaaagggggt cgccgtgacc
     1561 gcccaagacg accagttgat cgtcataggc cgccagttga tctccgaggc ggctcaaatc
     1621 tgcaatattg tacaactccc gaggtgggac acctgggggc cgcagcgtgc tctccggcag
     1681 gggagtcgca gttgtcagtt cgacttcgac ttcagccggt agcttgcgaa cgtgagcctg
     1741 ctgccgctcc cgaatgaatg cgcacagcgg tcctgattcg acctgctcgc ccagggtggc
     1801 aaaagcttgg tcgaggagtt ccgccgaaag gcgcgtatcc gcggcgaagc gggccgtcgt
     1861 ccgcgcaact tccgggtcga cctggaagca gctcggctgc gtgatcgggt cgccgcgcgg
     1921 catttccgga cgatatgaca gcagcagacc caccggaatg tcctgcgcaa gcaattggga
     1981 cactgcatcg cgcgtcagca ccaatccagc caatcccggc ggagcttgcg agaagattag
     2041 cccggcctga tccgcccgct cgcgcatctg cgctaccatt ccgctcgcca gccgggcatc
     2101 cagggcgggc tgatgaccgt ccaggcagag cacggcgtcg cactcgtaat gtttcagcac
     2161 gcgcgccacc ggcccagcct cgacatactc gtcaaaccac gtgctcccca gcaaactgcc
     2221 gcgccaagca tgcagattcc atttgcgggc gcagcgcagc agagcgcgcc ggcggcgtag
     2281 gccgtcgtcc aaggacagaa gctcgaaagc gggcgcgtgg ccggattcat ccagcgctgc
     2341 gcgggccgcc tcacgatcgc gcgggcgcac aaagaggcag cgcccgtcca gacccgtcac
     2401 gcgcgccgca cgatcgagcg tgtgctccaa gacgctgcgc gacccaagcc gggtacttag
     2461 ctgggaaggc cggcccagaa aggtgtcctg gaaatcggcg aaaacggcgg ccacgatttt
     2521 catcgcacac cctccccgag ccgctgcatc gcttgcggca aaacctgcat caggaattcg
     2581 cagccgtcga tgaatgcact cacgaactcg cgatcgcgcg ccagaccctg acggacggcg
     2641 tttgcgtctt cgtgctgctg cgcccgaatg cggcggtcgg ccgagtagcg gcgcagctcg
     2701 gccacttgcg acacgcccac caccatccca taaatctgct cataacgcct taaccgcacg
     2761 cgcagttcgt caactttcgg gaccagcttg ttgaattcgg ccggccggtc cacccgctcg
     2821 gccagttcgt cgagcaggcc gcgcatgatg cgcgcgattt ccgcgacttc cccgacgtcg
     2881 gccagccgct tctgaagggc ctcctgcgcc tgcacctgaa gctcgcgcga aagcgggtcg
     2941 ccgcccgcgg cgaacaagtc ggccggcagc gggcgcgtgc agaagcgctc gccggcctca
     3001 cgcaacttca tcggctcggt cccggccagt cgcatgccgc cttcgcaggc gtgaatcacg
     3061 cgcgcccgcg tgcgggcgaa gtcggtttgg aactgctcgg cgtaagtgaa gagctgatcg
     3121 tctgtatagg tcttgcgccc gtggacgtca gtgaccgttc gcagaatcgg tctggcgcgc
     3181 acgatgcgct cccactgctt catctcgacc gtgtaaaagc ggttcagttc cggggcccag
     3241 gtctgctcga tgggcagccc cggcgggtaa tacagcccgt cggagaacga caggtcctgg
     3301 cctatgagga tgatcgggtc gcaacccagg tactccgcca ggtagaacga cagatgtgcc
     3361 acggtgctgc cggccttcag cctgccgcgg acgggcgccg ccggccccag caccgcgtcg
     3421 cacaggttgt tgtgcagcac gtgcgtgcgc ccgcgatacg cctccaagac ctgccaggcc
     3481 gccttgggct cggccacgag aatgcagtcg cggaagtctt caacgccgtg aaagaactgg
     3541 gccgagattt cgtggaagtc caggctggtg acgaagtgcg ggggaatgcc gcgagccaac
     3601 aactgcttga acacggtttg aaccgcgatg atgaccgccc gctcgcgcag cccggcaagc
     3661 tggtcgatgt tgcgggcgag cgacggcccg gccgccacga gtatcgccgg gtatcccgcg
     3721 gcgcggttcc gcaaaacttc gacgcccggc cgcgccagat aggccggcaa gttgaagagt
     3781 atgtttttgc aggtaatgcg tgcattccgc agcacggtga ccatttgcag ccgcgcgtag
     3841 cagatgaagt cctgcaacgc gctgcgcgcc tggctatgga attccgcgcg atgttgggtg
     3901 gcgtacggca gcgcgattac tttgaggccc agcatgatgt cgatgtttgc gggccgcagc
     3961 acttcgtgta cgtgcgactt ttcggcggag gtcagaaaga acacgcgccc ttcgtgcagc
     4021 tcattcgaaa tgtccaccac cgtcagggcc gccttgatca gcgccaagtc ctgttcggcc
     4081 acgatcaccc gggcgacttc gtgccgccgc agcaactcgg ccaggtgata acccagccca
     4141 atcccgcaca gcaggaacgc gccgggggcg cgggcgccat ccgcgccatc ctctgcgcgc
     4201 gactgcgcct caacgaactg ctgggcttct tcaatggggc gatagcggct gtgcaagtaa
     4261 acggcgcggc cgccggcggt cggcagttgc gcggtgaagc gcccgtcccg cgtgcgctcc
     4321 agagccgggc aggatgagaa cggtacggcc tccaaacgga gggccagggc ctgctgccgc
     4381 cggtacagtt gggccaggtt cttgacgtac accgcgcgca gccggtcgag cgggctttct
     4441 tcgtgaccga gtggttcttt caggaaacag gcggccgtgt ccggcgggcg ctcgtccagg
     4501 gctacggaat tgtccatcat cgcaggtggc catccttggc tggcgtgcat ttcggtccac
     4561 agacttctat cgaccgctcg gttcggttcg acccaccgcc caggaacccg tcaatttcgc
     4621 gagcagaact gaccgataag aaagtggcca agacgccgtg agcggcgccg accgaaagag
     4681 gcgccgcccg aacagaggcg ccgtccgaac agaggctgcg ccgaccgaag agaggagccg
     4741 tcaatgacta cgaccacttg ttcgcgggag accgtggccc cgatacagga atctgccgct
     4801 tcttcgcggg agaatttggc cccgaagcaa aattctgccc ctacgcgaac cgccgagccc
     4861 gggaccacac tgccgctgga tgttttgagc gagcctgggg cgtacgtgtg caactggagc
     4921 gggcacctgc tgcgggtccg cccagacgca ttccccggcg ggcggccacc ggggttaaac
     4981 attgtgggcg atcgcccgct gacgataacg aaaatcagcg acgatcccga cgtagtcatt
     5041 tcgaaggcca agcgcctggc ggcgttcttc aacctggaag tgaaattctg accgcagcac
     5101 tgctcagccg gcagcacggc agcactgctc agccggcagc acagcggcca gcaccgctca
     5161 gccggcagca acgttaagaa ttcctgagca cagctataca cagaatttac ggccggtaag
     5221 ttcgcgtttt ctcgcgacgt aacgccattt ttggcgcacc ggcgcgcatg tcataaagtt
     5281 tatttgccgt tctcaaacgc gcgtaaatag tacgtattta gggtgtccgc tgctaaagtg
     5341 gcgagccgcg cgacatcgga gatcaaggag gatgcggcat ggctgttgag ccaatgacct
     5401 gtgcggagga gaccggcagc gaggaagggc ggatcgcgct gggggcgtgt gtaccatttg
     5461 acgtgataca cgaaccgggc gcctacgtat gcaactggag cgggcacctg ctccgagtcc
     5521 gcgcggatgc cctggagcgc gcccatgacg tgccggtcaa cctggtcggc cgagaaccac
     5581 tactcgtcac gaagatcagc agcaacccgc agatagcagt caaccaagcc cgctccatcg
     5641 ccagtcagct atccgtggcg gtcaacttct aacgcgcggg tc
//
LOCUS       DAOSYS010000232         5681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11928_length_5681_cov_5.558123, whole genome shotgun sequence.
ACCESSION   DAOSYS010000232 DAOSYS010000000
VERSION     DAOSYS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_5681_cov_5.558123"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1724)
                     /locus_tag="PLU99_12705"
     CDS             complement(84..1724)
                     /locus_tag="PLU99_12705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17953.1"
                     /translation="MPRLLAVLIVEDCEDDALLIVRELKRGGYEVVFERVDTAAAMQT
                     ALSQRNWDIIIADYAMPHFSGLAALKLLKECRQDLPFIIVSGAIGEDVAVEAMRLGAH
                     DYLIKGKLARLVPAVERELRDAEERHQRRRAQEELRAAHAELEKRVAERTAELAQANE
                     KLREEIAERQRIAEELKVANQAKDRFLAVLSHELRTPLTPVLLTASLLERRGDLPPDV
                     IEDVRTIRNETELEARIIDDLLDLTRVARGKMKFEFRRLDIHQLARRAVDSCAAYEGV
                     KIEMELTAEQPMVVADSVRLQQVFINLFNNARKFTRADGLITVRSFNPQPGEIRIEVT
                     DTGAGFRPELRERMFLPFEQGDDAVRPYPGGLGLGLAICKAIVEEHHGRIDAFSAGPG
                     RGATVAFNIPTLAPHAAADPADPRDGQRWHAGALRSLLVEDHRPTLDAMTKLLDGAGH
                     EVQTASTLATALEVGSRQRFDLVISDLRLPDGGGDELMRALSRRYGVTGIAVSGCGME
                     EDIRRSHDAGFVEHLTKPIDFGNLEAAIQRARSFIPAG"
     gene            complement(1735..2172)
                     /locus_tag="PLU99_12710"
     CDS             complement(1735..2172)
                     /locus_tag="PLU99_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO17954.1"
                     /translation="MTNQPILLVEDNPDDEALTLRAFRKCNIANEVIVARDGVEALEY
                     LFGTGRYAGREPREVPAVILLDLKLPKIGGLEVLRRLRADERTRLAPVVVLTSSREER
                     DVVNSYQLGANSYIRKPVDFEQFIEAVKQLGLYWLLLNEAVPR"
     gene            complement(2169..>4797)
                     /locus_tag="PLU99_12715"
     CDS             complement(2169..>4797)
                     /locus_tag="PLU99_12715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOO17955.1"
                     /translation="LVDRHGLVHRANPAACRLAGAPLQAVLSRRAGEILHCLGHLNDR
                     GGCGFGSECGHCPLGRAIAATIDAGQPQEGIKATLNVLGPAGAAKLDLLVSTAPLTLL
                     GQRLAVLFLQSCPPTEHSPSDSQRAQAFADSLIDTLPGVFYLFDANGRFLRWNKNFVA
                     VTGYAPAELARMTPWDFFRGDDCTRIREAVRRVFETGETQIEAGFVSKDGRVTPYLFT
                     GVRVQLAQGVCLAGVGMDISERKRVEQALKEASLAKQAEETLRLASRYNRSLIEASLD
                     PLVTIDADGKITDVNAATERVTGYSRHELIGTDFSDYFTEPEQARAGYQQVFREGVVH
                     DYPLEIRHRDGHRTPVLYNASVYKDEAGHVLGVFAAARDITAIKRAEESLRLASAYNR
                     SLIEASLDPLVTIDADGKITDVNAATERVTGYSRQELVGTDFSDYFTEPEKARAGYQQ
                     VFREGSVHDYSLEIRHRDGHHTPVLYNASVYRDAAGEIRGVFAAARDITELKRAQEAL
                     QTERQRLNEVFDLLPACVVLLSPDHRVPFANRFFRERFGESHGRRCYEILFGRSEPCE
                     TCETFEVLKDNSPHCWEWTGPDGRNYEILDYPFTDTDGSSLILEMGVDITERKRLELE
                     LRRLNEELEQRVAARTAELAASNKELEAFAYSVSHDLRAPLRAIDGFGQALLEDYADR
                     LDEEGRSHLQRTRAASQRMGQLIDGLLSLSRTTRSEIRRTYVDLSALAQMIAGELRKT
                     NPARQVEWDIAPGLIVHADASLMRIMLQNLLENAWKFTSKHPRAKIAVGAVEQNGETV
                     YFVRDDGAGFDMAYADKLFGAFSRLHAMAEFDGTGIGLATVQRIVHRHGGRVWAEGKV
                     EQGATIYFTIPRGFKELSR"
     assembly_gap    4798..4807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4968..>5681)
                     /locus_tag="PLU99_12720"
     CDS             complement(4968..>5681)
                     /locus_tag="PLU99_12720"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOO17956.1"
                     /translation="RKVRLLLKHLKPPLAYVPVDIAREQLYCSARALAEEHPYLQVHP
                     LCGDFTGPLSLPPNGALFSRRVVYFPGSTIGNFTPQEALRLLRQTAELCGPDGGLLLA
                     ADLRKDPRVIDAAYNDRQGVTAAFNLNLLTRMNRELGADFDVDKFWHHAFYDPRPGRV
                     EMHLVSRTDQRVLVCGHRFDFRAGESICTEHSYKYNFQDLCELASSAGFVVQQVWQDP
                     WKHVCMLNMAAGSRRITAV"
BASE COUNT         1009 a         1828 c         1740 g         1094 t
ORIGIN      
        1 cgaggcatca ttttcgtgtg tatcgggggt gctcatgtcc cgcatgatag gagaggagcg
       61 gatagaaatc agtaccccgg acgttagcca gccgggatga acgacctggc gcgctgaatc
      121 gccgcctcga gattgccgaa atcaatcggc ttggtcaggt gctcgacaaa gcctgcatcg
      181 tggctgcggc gtatgtcctc ttccatgcca cagccactaa cagcgatgcc cgtcacgcca
      241 tagcgccgcg acagcgcccg catcagctca tcgcctccgc cgtcgggcag gcggagatca
      301 ctgatgacca ggtcgaaccg ctggcggctg ccaacctcca gagcggtagc cagcgtgctc
      361 gccgtctgaa cttcgtgtcc ggccccgtcc aggagcttcg tcattgcgtc cagcgtgggc
      421 cgatggtcct cgaccaacag actgcgcaag gcgcccgcgt gccagcgctg gccgtcgcgg
      481 gggtctgccg ggtctgcggc cgcatgcggc gccagagtcg ggatgttgaa agccacggtg
      541 gccccgcgac ccgggccggc actgaaggcg tctatcctgc catggtgctc ctcgacgatg
      601 gctttgcaga ttgccagccc taggcccagg ccgccggggt aggggcgcac ggcgtcgtcg
      661 ccctgctcga aaggcaggaa catgcgctcc cgcaactcag gccggaagcc ggcgccagtg
      721 tcggtcacct caattctgat ctcgcctggc tgtgggttga acgagcgcac cgtaatgaga
      781 ccgtcggctc gtgtgaactt gcgcgcatta ttgaacaggt tgatgaacac ctgctgcaag
      841 cgcacagaat ctgccacgac catcggctgt tcggctgtca gctccatttc gattttgacg
      901 ccctcgtacg ccgcgcagct atcgacggcg cggcgggcca gttgatgaat atcgaggcgg
      961 cggaactcga acttcatctt gccgcgcgcc acgcgggtga ggtccagcag gtcgtcaatg
     1021 atgcgtgctt cgagctcggt ctcgttgcgg atagtccgca catcctcgat tacgtccggc
     1081 ggcaggtcgc cgcgccgctc cagcaaggac gcggtgagca gcaccggcgt cagcggcgtg
     1141 cgcagttcgt ggctcagcac ggccagaaag cggtccttgg cctggttggc gaccttcaac
     1201 tcctcggcga tgcgctggcg ctcggcgatt tcctcacgca gcttttcgtt ggcctgggcc
     1261 agttcggccg tgcgttccgc gacgcgtttt tccagttcgg cgtgcgcggc gcgcagctcc
     1321 tcctgggccc ggcggcgttg gtggcgctct tccgcgtcgc gcagttcgcg ctcgaccgcc
     1381 ggaaccaggc gggccagctt gcccttgatg aggtaatcat gcgcgcccag ccgcatggct
     1441 tcgacggcga catcctcgcc gatcgcgccc gacacgatta tgaagggcag gtcctgccgg
     1501 cattctttta gcagcttcag cgccgccagc ccgctgaagt gcggcatcgc ataatctgcg
     1561 atgatgatgt cccagttgcg ttgcgacagg gcggtctgca tggcggcggc cgtgtcgaca
     1621 cgttcaaaga ccacctcata accgccgcgc ttcaactctc gcacgatcag cagcgcgtcg
     1681 tcttcgcagt cttcgactat caatacggca agcaatctgg gcatgctctg tccctcagcg
     1741 gggtactgct tcattcaaaa gcagccaata gaggccaagt tgcttgactg cttcaataaa
     1801 ctgctcgaag tccaccggct tgcggatgta gctgttagcc cccaactggt agctgttgac
     1861 gacgtcgcgc tcctcgcgcg aagaagttaa taccaccacc ggcgccagcc tggtgcgctc
     1921 atccgcccgc agccgccgca gtacttccag cccgccgatt ttcggcagtt tcaagtccag
     1981 caatattacc gccggcactt ccctcggctc gcggcccgca tagcggccgg tgccgaagag
     2041 gtactccaat gcctcgacgc cgtcgcgcgc cactatgacc tcgttggcga tgttgcactt
     2101 gcggaaggcg cgcagcgtaa gggcctcgtc atcggggttg tcttcgacga gaagtattgg
     2161 ctgattcgtc accggctgag ctccttaaat ccgcgcggga ttgtaaagta aatcgtcgcc
     2221 ccttgctcca cttttccctc cgcccatacc cgcccgccgt gccgatggac gatgcgctgc
     2281 acggtagcca gcccgatgcc ggtcccgtcg aattcggcca tcgcgtgcag ccggctgaag
     2341 gcgccaaaca gcttgtccgc gtacgccatg tcaaagccgg ccccatcgtc ccgtacgaaa
     2401 tagaccgttt cgccgttctg ctccaccgcg cctacggcga tcttggcccg cgggtgtttg
     2461 ctggtgaatt tccaggcgtt ttccagcaga ttctggagca tgatgcgcat taagcttgcg
     2521 tcggcatgga cgataagccc gggcgcaatg tcccattcga cctgtcgcgc cggatttgtc
     2581 ttgcgtagtt cgccggcgat catttgcgcc aacgcgctaa gatccacata cgtcctgcgt
     2641 atttcgctgc gcgtcgtgcg cgacaaactc agcagcccgt caatcaattg ccccatacgc
     2701 tgactggcag cgcgggtgcg ctgcagatgg ctccggccct cctcgtccag gcggtcggca
     2761 tagtcctcca gcagcgcctg accgaagccg tctatggcgc gcagcggcgc ccgcaggtcg
     2821 tgtgagaccg aataggcgaa cgcctccaat tccttattac tggccgccaa ttccgccgtg
     2881 cgcgccgcaa cgcgttgctc cagttcctcg ttcaaccggc gcagctcgag ctccagccgc
     2941 ttgcgctcag tgatatccac gcccatttcc aggatcagac tggacccgtc tgtgtcggtg
     3001 aacggataat ccaatatttc gtagttgcgc ccatccggac cggtccactc ccaacaatgc
     3061 ggggaattgt cttttaacac ctcgaatgtt tcgcaggttt cgcacggctc gctgcgccca
     3121 aagaggattt cgtagcagcg ccggccgtgc gattccccga accgctcgcg gaaaaagcgg
     3181 ttggcaaatg gcacgcgatg gtcgggcgag agcagcacca cgcaggccgg taacaagtcg
     3241 aacacctcgt tcaagcgttg ccgctcggtt tgcagcgcct cctgggcccg tttgagctcg
     3301 gttatgtcgc gggcggccgc gaacacgccg cgaatctcgc cggccgcgtc ccggtatacc
     3361 gaggcgttgt agagcaccgg cgtgtgatgg ccgtcgcggt ggcgaatctc cagcgagtag
     3421 tcgtgaaccg agccttcccg gaaaacctgc tggtagccgg cacgggcttt ctcgggctcg
     3481 gtgaaatagt ctgagaagtc tgtgccaacc agttcctgac gggaatagcc ggtgacccgc
     3541 tcagtggcgg cattgacgtc cgtgatcttg ccgtccgcgt caatggtgac cagcgggtcc
     3601 aggctggcct caatcaggct gcggttgtat gcgctggcca gccgcagcga ctcttcggct
     3661 cgcttgatgg cggtgatgtc acgggcggcc gcgaacacgc cgagcacgtg tccggcctcg
     3721 tccttgtaga cggaagcgtt atagaggacc ggcgtgcggt gcccatcgcg atgccggatc
     3781 tccagtggat aatcgtgaac gaccccttcg cggaatacct gttggtagcc cgcccgggcc
     3841 tgctccggtt cggtgaagta atcggagaag tccgtaccga tcagctcgtg acgcgaataa
     3901 ccggtgaccc gttcagtggc cgcattgacg tccgtgatct tgccgtccgc gtcaatggtg
     3961 accagcgggt ccaggctggc ctcgatgaga ctacggttat agcggctggc cagtcgcagc
     4021 gtctcttcgg cctgcttggc aagactggcc tccttcagcg cttgctcgac tcgcttgcgc
     4081 tcggagatat ccatgccgac gccggccagg cacacaccct gcgccaattg aacgcgcacg
     4141 ccggtgaaca gatacggtgt gacgcggccg tctttcgaaa cgaaacccgc ttcaatctgc
     4201 gtttcgcccg tttcgaagac ccgccgcacg gcttcccgga ttcgcgtgca atcatcgccg
     4261 cggaagaaat cccacggcgt catgcgcgcc aattcggccg gcgcgtaacc cgtcaccgcc
     4321 acgaagttct tgttccagcg tagaaaccgc ccattggcgt caaacagata aaacacgccc
     4381 ggcaaggtgt cgatcagcga gtccgcaaac gcctgtgcac gttgcgaatc gctgggacta
     4441 tgctcagtcg gcgggcacga ctgcagaaat agcacggcca ggcgctgccc aaggagtgtc
     4501 agcggtgcag tggagacgag caggtcgagc ttggcagccc ctgccggacc gaggacgttc
     4561 agggtcgcct tgattccctc ttgcggctgc cctgcgtcga tagttgccgc aatggctctg
     4621 ccgagcgggc agtgcccgca ctccgaccca aagccgcacc cgccgcgatc attcaaatgc
     4681 ccgagacagt gcagaatctc ccctgcgcgc cggctgagca cggcctgcaa aggagcgccg
     4741 gctaaccggc atgccgccgg gttggcccga tgcacgaggc cgtggcggtc aaccaggnnn
     4801 nnnnnnnccc gtccaggtcc gtcgcgttct gggtgagcgg caaggctttc gttcctcaag
     4861 gttggtccgc cgataactgt tgcgcgcgta gtgggcgaat tgcggcgcgc gaaacagcga
     4921 taaacatttt ccgcgtgcgc agcggaggcg tcaagccaag attggtatta caccgctgta
     4981 atgcggcggg aaccggccgc catgttcagc atgcagacgt gtttccaggg gtcctgccat
     5041 acctgctgga cgacgaagcc ggcgctggac gccagctcac atagatcttg gaagttgtac
     5101 ttgtatgaat gttcggtgca aatcgattcg ccggcacgaa agtcaaaacg atgcccgcac
     5161 accagtacgc gctggtctgt gcggctaacc aggtgcatct cgacgcggcc ggggcgcgga
     5221 tcgtagaagg catggtgcca gaatttgtcg acgtcgaagt ccgcgcccag ctcgcggttc
     5281 atgcgggtaa gcaggttcag attgaacgcc gcggttactc cctggcgatc gttatacgcg
     5341 gcatcaatca ctcgcgggtc tttcctcaag tcggccgcca gcaacaggcc gccgtccggg
     5401 ccgcacagtt cggccgtctg ccgcaacagc cgtagcgcct cttgcggagt gaaattccct
     5461 atggtcgaac cgggaaagta gaccacccgc cggctgaata gcgctccatt gggcggaagg
     5521 gacaggggcc cggtgaaatc gccacacagc ggatgcacct gcaaatacgg gtgctcctct
     5581 gccagggcgc gcgccgagca atagagctgt tcgcgggcaa tgtcgaccgg cacgtatgcc
     5641 aggggcggct tcaagtgctt gagcagcagc cgcaccttgc g
//
LOCUS       DAOSYS010000233         5671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11951_length_5671_cov_5.740385, whole genome shotgun sequence.
ACCESSION   DAOSYS010000233 DAOSYS010000000
VERSION     DAOSYS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11951_length_5671_cov_5.740385"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLU99_12725"
     CDS             complement(<1..836)
                     /locus_tag="PLU99_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOO17957.1"
                     /translation="MNLLLANAWLPTFSDLRLFSPELVLVAAIAALLLAPLFLGRSAY
                     VAALIAMAGAFAGLLATGQVAVSVAAAGQADMAAPGAYPMLIADGFSVFFKLFLLLLL
                     VLVIWLWTVGASSREMNAYERAPRAGWPGQPDVPGAIGSPEFFVLLLASALGMMLMVG
                     TLNLLVMIVAMETASLPSYAMAASNRRSRLGAEAGLKYVMFGAAAAAIMVYGVSLLYG
                     QFGTFDLGRIAEQTAGGVADAEAGAGPALPVFWLGLAAIGAGVAFKIAAFPMHYWCPD
                     VF"
     gene            complement(880..2508)
                     /locus_tag="PLU99_12730"
     CDS             complement(880..2508)
                     /locus_tag="PLU99_12730"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOO17958.1"
                     /translation="MEYGNLLNWIIFIPTIAAVLCLIVPNTRAVKVLAVLGSGLTLLL
                     SLSLFGHFAWWSGEGPGEIFGSGYGQKLYEVTKLTWINLGSGDSKSAIEYFVGVDGLS
                     FPLIILTTVVSFLACLASWNIEHWKINKGIRGYFTLFLLLETGMLGVFCALDFFLFYI
                     FWEVMLLPMYFLIGVWGGPRKEYAAIKFFIYTLVGSVLMLVVLLAFYFYAPAGMRTFN
                     LIEMANNSAIQSAFGSATFMGLRFAPFMFVLLYIGFAIKVPVFPFHTWLPDAHVEAPT
                     PISMILAGVLLKMGAYGLMRISYPILPQAGVWAAYGLALLGVINIIYGALCAMAQTDF
                     KRLVAYSSISHMGYVLLGLAIMTPGGWEGAMFQVIAHGISSPMCFFLVGVIYDRAHHR
                     EINRFGGLWLSMPVYGSLATLGFFASLGLPALCGFIGEFWVLVATFSASQWTWAKPVA
                     VVAATGVVLTACYILWMMQRVYLGPRRPEYEGYPDASAREIAVLVPFAALAILLGILP
                     QQSLFNFMNGTLNQITELFARHGGTIVSAMNHLP"
     gene            complement(2623..4944)
                     /locus_tag="PLU99_12735"
     CDS             complement(2623..4944)
                     /locus_tag="PLU99_12735"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOO17959.1"
                     /translation="MSNIELLLVAGVLIPLGGFVLLALFGSRLGKPLAGWVAVAAIAT
                     SCCLSTAALVQWQGMDSAARLAARTGPYLWAHLGGVPLTFGVNLDSLTLIMFFMVSFI
                     STWIFVFSVGYMAGHSDEIDKVSKFHRFFTYLCLFCFSMLLLVIADSLLLLFIAWELV
                     GLCSYLLIGFYFHKKSASNAAIKAFVTNRVGDFAFIIGISLVYLFLHNLSLKGAADAF
                     HQQWEAGGPLFTKTFLGLNLATWMGMLLFCGAIGKSAQFPLHVWLPDAMEGPTPVSAL
                     IHAATMVAAGVYLTARIFVLLTPEAQLFVAIIGCITLTLAALVAIVQTDIKKVLAYST
                     ISQLGYMMLGMGVGAWIAALFHLLTHAFFKALMFLGSGQVIEGTHHEQDIRRFGGLRR
                     KMPVTCWTFFIGVLAISGAGIPMLHFGLGGYFSKDEILAVAWHRAFGREHALHEEHSR
                     AEPGHVTPEYATAVQSWQMQPAAQVKLAAYTEPGAAPDEPAGISFGAAPVHVRPAAHV
                     EKAGPDWLWVLLFVVPVAMAYITPFYMMRVWWLTFMGKPRDQHVHEHAHESALMYVPL
                     IVLAIGTVFASYWLFRPMVAEAIPQPFLTAAFDGVAANAASAHGALIDHDAHAAMARI
                     VGFAFLFAFAFAIAMYWQGLTFASRVAGGLRPLHTLLVKKFYFDEAYGLVLIGGTMAL
                     KGLAYAFDKYVVDGLVNLSAWVTERISRFSGVVLDAGLVDGLVNLVGRGTYGIGGVVR
                     APQSGRIRNYVLYAAGGVALVAISMALWACRGS"
     gene            complement(5050..5361)
                     /gene="nuoK"
                     /locus_tag="PLU99_12740"
     CDS             complement(5050..5361)
                     /gene="nuoK"
                     /locus_tag="PLU99_12740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOO17960.1"
                     /translation="MLTVSLTHYLVLAAFLFCCGLYVMTTKRNAVGILIGAELVLNAA
                     GLNMVAFSRYGIGGIDGQIGTLFVIVLAAAEAAIAIAILVNYYRVLATIDVDQADALK
                     G"
     gene            complement(5455..>5671)
                     /locus_tag="PLU99_12745"
     CDS             complement(5455..>5671)
                     /locus_tag="PLU99_12745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOO17961.1"
                     /translation="LAGLVGILATTQWHAADSTALASGQASDDVAAFGKQLLGPYLVP
                     FELVSVLLLAVMIGAAYLAQPARREAE"
BASE COUNT         1196 a         1776 c         1785 g          914 t
ORIGIN      
        1 tcgaaaacgt ccgggcacca gtagtgcatc gggaaggcgg cgatcttgaa cgcgacaccc
       61 gcgccgattg ccgccagacc cagccagaac actggtagcg ctggccccgc accggcctcg
      121 gcatcggcaa ccccgccggc agtctgctcg gcgatgcggc ctagatcgaa cgttccgaat
      181 tgcccgtaga gcaacgatac gccataaacc atgatggctg cggccgccgc gccgaacatc
      241 acgtatttca gtccggcttc ggcgcccagt cgcgagcggc ggttgctggc agccatggcg
      301 tagctcggca acgaagcggt ctccatggca acgatcatga ccaggagatt cagcgtgccg
      361 accatgagca tcatgcccag cgcgctggcc agaagcagta cgaagaactc gggcgagccg
      421 atggcgcccg gaacatccgg ctgaccgggc cagccggcgc gcggcgctcg ctcgtaggcg
      481 ttcatctccc ggctgctcgc gccgacggtc caaagccaga tcaccagcac cagcaaaagc
      541 agcaggaaca gcttgaaaaa gacgctgaag ccatcggcga tcagcatggg gtaggcgccg
      601 ggcgcggcca tgtccgcctg acccgcggcg gcgacgctca ccgccacctg gccggtagcc
      661 aacagtccgg cgaacgcgcc ggccatggcg atcagggcgg cgacgtaagc gctgcgccca
      721 aggaagagcg gcgccagcag caacgccgcg atggccgcga ctaacaccag ctcagggctg
      781 aacagccgca agtcgctgaa cgtcggcaac caggcattcg caagtaatag gttcattgcg
      841 ttatccgact ccgggtctgt ctttctgcgg ctgcattcct tacggcaagt ggttcatggc
      901 gctgacgatc gttccgccgt gccgggcgaa gagttccgtt atctggttca gtgtgccgtt
      961 catgaagtta aagaggctct gctgcggcag gatgccgagc agaatcgcca gggccgcgaa
     1021 cgggaccagc accgcgattt cgcgggccga ggcgtccggg taaccctcgt attccgggcg
     1081 gcgcgggcca aggtagacac gctgcatcat ccacaggatg tagcaggcgg tcagaaccac
     1141 gcccgtcgcg gccaccaccg ccactggctt ggcccaggtc cactggctgg ccgagaaggt
     1201 cgccaccagc acccagaatt cgccgatgaa gccgcacaac gccggcaggc cgagcgaggc
     1261 gaagaagccc agcgttgcca gcgagccgta aacgggcatg ctcagccaca ggccgccgaa
     1321 gcggttgatc tcgcgatggt gagcgcggtc gtaaatgacg ccgaccagga agaagcacat
     1381 cgggctggaa atgccgtggg cgatgacttg gaacatcgcg ccttcccagc cgccgggcgt
     1441 catgatggcc aagccgagca gaacgtagcc catgtggctg atcgagctgt acgcgaccag
     1501 gcgcttgaag tcggtttgag ccatggcgca caaagcgccg taaatgatgt tgattacacc
     1561 aagcagggcc aaaccgtatg ccgcccagac gccggcctgc ggcaggatcg ggtagctgat
     1621 acgcatcagg ccgtaggcgc ccatcttcag cagcacgccc gccaggatca tgctgatcgg
     1681 ggtgggggcc tcgacgtggg cgtcgggcaa ccacgtatgg aacgggaaca ccggcacctt
     1741 gatggcgaag ccgatgtaca gcagcacgaa catgaagggg gcgaagcgca gccccatgaa
     1801 cgtggcgctg ccgaaggccg actgaatggc cgaattattg gccatctcga tcaggttgaa
     1861 ggtgcgcatg ccggccgggg cgtagaagta gaacgccagc aggacgacca gcatcagcac
     1921 gctgccgacc aaggtgtaga tgaagaactt gatcgcggcg tattccttgc gcgggccgcc
     1981 ccagacgccg atgaggaagt acatcggcag gagcatcact tcccagaaga tgtagaacag
     2041 gaagaagtcc agcgcgcaga acacgcccag catgccggtt tcgagcagca ggaacagcgt
     2101 gaagtagccg cggatgccct tgttgatctt ccagtgctct atgttccagc ttgccaggca
     2161 ggccaggaag ctgacgacgg tcgtcaggat aatcagcgga aagctcaggc cgtccacacc
     2221 gacgaagtac tcgatggcgg acttgctgtc gccgctgccg aggttgatcc acgtcagctt
     2281 cgtgacctcg tacagcttct ggccgtagcc gctgccgaag atctcgccgg gcccctcgcc
     2341 gctccaccag gcgaagtggc cgaagagcga tagcgacagc aacagcgtca ggccgctgcc
     2401 cagcacggcc agcactttaa cggcccgcgt gttgggtacg atcaggcaca agacggccgc
     2461 gatggttggg atgaagatga tccagttcag gaggttgccg tattccacgg atggttcctc
     2521 gttctcaaaa gtgattagtt attagtcgtt agtattgaga aaatcgtccg atcgccacaa
     2581 tcatccgacc gccaatactg actaacaact aacaacttct tcctaactac ctctgcatgc
     2641 ccagagggcc atgctgatcg cgaccagcgc tacgccgccg gccgcataga ggacgtaatt
     2701 ccgaatcctg ccggactgcg gcgcccgcac gacgccgccg atgccgtatg ttccgcggcc
     2761 gaccaggttg accaggccgt cgaccaggcc ggcgtcgagc accacgccgc tgaagcgtga
     2821 gatacgctcg gtgacccagg ccgacaggtt gaccaggccg tccacgacgt atttgtcgaa
     2881 cgcgtacgcg aggcctttga gggccatggt gccgccgatc agcaccagac cgtaggcttc
     2941 gtcgaagtag aatttcttca ctaagagcgt gtgcagcggg cgcaggccgc cggcgacgcg
     3001 gctggcgaac gtcaggcctt gccagtacat ggcgatcgca aacgcgaacg cgaacaagaa
     3061 ggcaaagccg acgattctgg ccatagcggc gtgcgcgtca tggtcgatca gtgcaccgtg
     3121 cgcacttgcc gcgttggcgg cgacgccgtc gaacgccgcg gtcaggaacg gctgcgggat
     3181 cgcctcggcc accatcgggc ggaaaagcca atagctggcg aagacggtgc cgatagccaa
     3241 gacgatcagc ggcacgtaca tgagggcact ttcgtgtgcg tgctcgtgca cgtgctggtc
     3301 gcgcggcttg cccatgaagg tcagccacca gacgcgcatc atgtagaagg gcgtgatata
     3361 cgccatcgca accggtacga cgaagagcag cacccagagc cagtccggac cggctttctc
     3421 aacgtgcgca gcgggacgaa cgtgaactgg tgcggctccg aaagagatgc cggcgggttc
     3481 atcgggcgcg gcgcccggct ctgtatacgc agccagcttc acctgcgccg cgggctgcat
     3541 ttgccacgac tgaaccgcag tggcgtactc cggggtgaca tgccccggct ccgctcttga
     3601 gtgttcctca tgcagcgcgt gttcgcgtcc gaacgcccgg tgccacgcga cggcgagaat
     3661 ttcatccttg ctgaagtagc cgccgaggcc aaagtgcagc atcgggatgc cggcgccgga
     3721 aatcgctagc acgccgatga agaaggtcca gcaggtcacc ggcatcttgc ggcgcaggcc
     3781 gccgaatctg cggatgtcct gctcgtggtg cgtgccttcg atgacctggc cggagccgag
     3841 gaacatcagg gccttgaaga aggcgtgcgt cagcaggtgg aagagggccg cgatccaggc
     3901 gcccacgccc ataccgagca tcatgtagcc gagctggctg atggtgctgt atgccagcac
     3961 cttcttgatg tcggtttgca cgatggcgac cagggcagcc agtgtcaggg ttatgcagcc
     4021 gatgatcgcg acgaagagct gcgcttcggg cgtcagcagc acgaagatgc gggccgtcag
     4081 atagacgccg gcggccacca ttgttgccgc gtggatcaac gccgagacgg gcgtcgggcc
     4141 ttccatcgca tccggcaacc acacgtgcag cgggaattgg gcgcttttgc caatcgcgcc
     4201 gcagaagagc agcatgccca tccaggtcgc caggttcagg cccaggaacg ttttcgtgaa
     4261 cagcgggccg ccggcttccc attgctgatg gaaggcgtcg gccgcgccct tgagcgaaag
     4321 attgtgcagg aacagataaa ccaggctgat cccgataata aaggcgaaat cacccacgcg
     4381 gttagtgaca aacgccttta tcgcagcgtt gctggcgctc ttcttgtgga aatagaagcc
     4441 gatcagcagg tacgagcaca ggcctaccag ttcccacgcg atgaacagca gcagcaggct
     4501 gtcggcgatg accagcagca gcatgctgaa gcagaacagg cagaggtagg tgaagaagcg
     4561 gtggaacttg ctgaccttgt cgatttcgtc actgtggccg gccatgtagc cgacgctgaa
     4621 cacgaaaatc caggtcgaga tgaaactgac catgaagaac atgatcagcg tcaggctgtc
     4681 gaggttgacg ccgaaggtga gcgggacgcc gcccaggtgc gcccacaggt agggtccggt
     4741 tcgcgccgcc aagcgggccg cggaatccat tccttgccac tgcaccagcg ccgccgtact
     4801 gaggcagcag ctcgtcgcga ttgccgcgac cgccacccag cccgccagcg gcttgcccag
     4861 ccggctgccg aatagcgcca gcagcacaaa gccgcccagc ggaatcagga cgccggccac
     4921 aagcagtagt tcgatgttcg acattggcgc tctcggtttc agcggtcagg tgtcagttcg
     4981 cgcgcctgga cgcttgcccc gcgaatggac ctccggcgca acggacaacc aagaactgac
     5041 aactgacaac tatcctttca acgcgtcggc ctggtcgacg tcgatcgtcg ccagcacccg
     5101 gtaatagttc actaatattg cgatcgcgat ggccgcctcg gccgccgcca gcacgatcac
     5161 gaacaacgtc ccaatctgcc cgtcaatccc gccgatgccg tagcgcgaga acgccaccat
     5221 gttcaacccg gcggcgttca gcaccagttc ggccccgatc aggattccga ccgcgttccg
     5281 cttagtggtc atcacgtaca gaccgcagca gaagaggaag gcggcgagga cgaggtagtg
     5341 ggtcaggctg accgttagca tcgtaggtct aagctccgtg tgctcagttt gaattcaaaa
     5401 tgccgaatga agcttcgcgg cactgcattc tgaatttctc accttgcatt gtttttactc
     5461 cgcttcccgc cgggccggct gggcgaggta cgccgcgccg atcatcaccg ccagtagcag
     5521 cacgcttacc agttcgaacg gcaccaggta cgggccgaga agctgcttgc caaaagcggc
     5581 gacgtcgtcg ctcgcctgcc cggaggccag ggccgtgcta tcggcggcat gccattgcgt
     5641 cgtcgccagg attcccacca accccgccag c
//
LOCUS       DAOSYS010000234         5658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_11990_length_5658_cov_5.427985, whole genome shotgun sequence.
ACCESSION   DAOSYS010000234 DAOSYS010000000
VERSION     DAOSYS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11990_length_5658_cov_5.427985"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..2188
                     /locus_tag="PLU99_12750"
     CDS             275..2188
                     /locus_tag="PLU99_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOO17962.1"
                     /translation="MLEHLAGMSLPGCGEGSPCAQAAASVWGRVKLGSFEWPVAYLGL
                     AYFLAALITWLALRGAVSPAFRYLVRLGALISLGFCAIIVATRMVCYYCLAAHLANFA
                     FWITIELAAIRPARPHRAAVVLGSVFALTSLVLGIWDASHRGAVAERAEQDRSAAAQE
                     IIERSRQIVSGPTPSPAPAPAPAPSQSPDATPLDSAGAQRPQPPSPGQPRQAPVAAAP
                     QPEQVEPAPDASPDAGPPMFTGRYRVGPAEAPIRIVCFTDYQCRDCYNIEKQLVKLYE
                     TRDDISISIKHFPFNADCNPGAARTLHPNACWAARAAEAAGKLWGPEGFWKMHVWLFE
                     RRGEFQNLEELETAIREFGYDPAGFAETMGSPQILEVIKADAVEAKQLGLYFTPMIFI
                     NGVELKGWMAPNALIRTVEQVAATNPPPRSAAYDQPPMAFEKYVADWRDGKQIKLPSD
                     KQAWTLGTPDAPVEIVLWGDYQEHYTSRADAVIRQFVAQHNNVRYTYRHYPFNSDCNP
                     NLKDRRFPNACLAAKAAEAAGILGGNEGYWKMHTWLFEHRQDLTEESLSAAAAELGFG
                     AAMLLAAMEQSEVQDNIQDDIDAAKKLPVLRYGTPPGLHSIPTVFVNGRHIPRWELGD
                     QPVLETILKEAAQ"
     gene            complement(2425..2497)
                     /locus_tag="PLU99_12755"
     tRNA            complement(2425..2497)
                     /locus_tag="PLU99_12755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2462..2464),aa:Gly,seq:tcc)
     gene            complement(2644..5517)
                     /locus_tag="PLU99_12760"
     CDS             complement(2644..5517)
                     /locus_tag="PLU99_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17963.1"
                     /translation="MSGFEFRVLGFKSLRVLLLVGLCVSSSAGAEPTELPADLLQAVQ
                     EGRPEVLARFGETSRPEECRLLAQAYANLARRVADATERSQACQRSAEWYERWIAAVD
                     QAAKGDTSPQTLLEQAAARLEYASMVMNLQAAGELDRFEITAGQQGDRAKLLAWLGTA
                     RIQCRRAELLIEPVHSALRQREDEFLAAGIYDSIVQTKFDAEFNDAWAGYYCAVLDER
                     NQEARLEALSDAENAFRMLLEAGRAKQARGRCLLGLALAQRELGRLGEAERNFAAALK
                     AESAPLEAARIRYEWARCHLASGKFAEARALLRPLLELATAEPSPEQQVVQFYINLAR
                     LWDANSYLLEARALEAEAGGAGGDPALRQQAQQAREIALRKFEVMAARGGPWPALVQL
                     YIGASIDLNADPQRLSPGELLYAARHFAEAGQLEEARQRLEAALGRPEFALQKSGTDE
                     PRGAAPGVSSESRELAGQLLFELAKCYYRLDRPVDAAETFDVLSREYRSHRLAPQAAT
                     FAYQLWAEVAQSSRSRSAYERLAETLVNLIQSFADHPQRQRAAWLLPVAWQAAGRYAA
                     AADEFAKVGRDSQYWEEAQGRLRVCRRLALEQRRAELSGAEFQRQARALADELTAYAD
                     QALQRAAAGAGDAQQARSWSAEARVSAAELLVGQDDEAALAALERFDEQYPHDERVGR
                     VLAVRIRALRGLKRFDEAARVLGRYLEAVSAEQSGPVLNLLTRGMQEEVERLTLEGEF
                     DAARRLALESLPAFEQLERWVRQEPSRAAAVAPATLARAQMLYTAGELEQARERLVEI
                     LANDAHNGHAQRLLALVLTAQLPPAGSGADAAELRADSDQPKADSEQPRADSAQFKTD
                     SEQPKAADSAQLRAASEQLKAAQEAWAVLLKDSGLRQRSPEHYWEARYNWLALLLRQG
                     KAAEVERAIDQEQVWYPALGGANWREKLLRLRDEARSLSLKGE"
BASE COUNT          972 a         2074 c         1660 g          952 t
ORIGIN      
        1 gcgagagcga aaaggacgcc ctcgtgcgca agcacctggg ctattgacgc ctgccccagc
       61 acgttccaat ggccgcacac caccgaacca aacgcatacg agccataccc ggagacccac
      121 tgtgcacact gaggagtcat cgaggaaacg acgtcgccta gccacgccgg ccgaccgttc
      181 gtcagcggcc gccgccggga ctgccccctc cattatctcg ctgatcgttg ccagtatctt
      241 cgccgcgctc gccaccttgg cctccctggc actggtgttg gaacatcttg ccggcatgag
      301 cctgcccggc tgtggggaag gcagcccttg cgcccaagcg gccgccagcg tctggggccg
      361 agtcaagctc gggtcgtttg agtggccggt tgcctacttg ggactggcgt atttcctcgc
      421 cgccctcatc acgtggctcg cgctgcgtgg cgccgtgtcg cccgcctttc gctacctcgt
      481 gcgcttgggc gcgctgattt ctcttggctt ctgcgcgatc attgtggcca cgcggatggt
      541 ctgctactat tgcctcgccg cgcatctggc caactttgcc ttctggatca cgatcgaatt
      601 ggccgccatt cgcccggcaa gacctcaccg cgccgcggtc gtccttggca gtgttttcgc
      661 ccttacctcg ctcgtactcg gcatttggga tgcctcgcac cgtggcgcag tcgccgaacg
      721 ggctgaacag gaccgctccg cggcggcgca ggaaatcatc gaacgctccc gccagatcgt
      781 gtcgggaccg accccttcgc cggctcccgc accggccccg gctccatcgc agtctccgga
      841 cgccacgcct ctcgatagcg cgggcgcaca gaggccacag ccgccgtctc ccggtcagcc
      901 ccggcaagcg cccgtcgccg ccgctccgca accagagcag gttgagcctg cgccggacgc
      961 ctcgcctgat gccggcccgc ccatgttcac cggccgctat cgcgtcggcc ccgccgaagc
     1021 accgatccgc atcgtttgct tcactgacta tcagtgccgg gactgctaca acattgagaa
     1081 gcagctcgtc aagctctacg aaacgcgcga cgatatctcg atttcgatca agcacttccc
     1141 cttcaatgcc gactgcaatc ccggcgccgc cagaaccctg caccccaacg cctgctgggc
     1201 cgcccgcgcc gccgaagcgg ccggcaagct ctggggaccc gaaggcttct ggaaaatgca
     1261 cgtctggctg ttcgagcgcc gcggcgaatt ccagaacctc gaggaacttg agacggccat
     1321 tcgcgagttc ggctacgatc cggccggttt tgcggagacg atgggaagtc cgcagattct
     1381 ggaggtgatc aaggccgacg ctgtcgaggc caagcagctc ggcctgtatt tcacgcccat
     1441 gatcttcatc aacggcgtgg aattgaaggg ctggatggcg cccaacgccc tgatccgcac
     1501 ggttgagcag gtggcggcca ccaacccgcc gccgcgctcc gccgcctacg accagccgcc
     1561 gatggcgttt gagaagtacg tcgccgactg gcgcgacggt aaacagatta agttgccttc
     1621 cgataaacag gcctggacac tgggcacgcc agacgcgccg gtcgagatcg tcttgtgggg
     1681 cgactaccag gaacactaca cctcgcgcgc cgatgcagtc attcgccagt tcgtcgccca
     1741 acacaacaac gtgcgctaca cgtaccggca ctatcccttc aactccgact gcaacccgaa
     1801 cctcaaggat cgccgcttcc cgaacgcgtg cctggccgcc aaggccgccg aggccgccgg
     1861 aatcctgggc ggtaatgagg gctactggaa aatgcacacc tggctgttcg agcatcgcca
     1921 ggacttgacg gaagaaagcc tctcggctgc ggctgccgag ttgggcttcg gcgcggccat
     1981 gctgctggcg gccatggagc aatcagaggt gcaggacaac atccaggacg acatcgatgc
     2041 cgccaaaaag ctgccggtgc tgcgctacgg cacgccgccc ggcctgcact cgatcccgac
     2101 cgtcttcgtc aacggccgcc acatcccgcg ctgggaacta ggcgaccagc cggtgctgga
     2161 gaccatactg aaggaagcgg cgcaataacc cgttaagcgg cgcagtgcat tcctgagcat
     2221 cgcaatgcgt cgcatgcgca gcgtcacatg gggtgtgcca ctgcttgaaa gcgcagcctc
     2281 aagcagtgct agcccgacct tcgcacctca ggcgaattcc gcgaagtggc ggcagtccgg
     2341 ggcgacacgc cccggctctg ctctgcgacg ccgtcctgcc gacgggtcgc tctaactatg
     2401 cagctcggtc aggtttgcgt tcaaagcggg cgaccgggtt cgaaccggca acattcagct
     2461 tggaaggctg atgctctacc aattgagcta cgcccgcata cgccgcagcc ggcggcagca
     2521 ttgccacggc cgacgacagc atcgccagcc gaatggcatt ataacctgag ccgacgggac
     2581 ggccagcacc aagagccgct ccagccgctc cagaagggta gcttcagcgc ccggcagggc
     2641 gcctcactcc cccttcaacg acagcgaacg agcctcatcc cgcagcctga gcagcttctc
     2701 ccgccaattc gcaccgccca acgccggata ccagacctgc tcctggtcga tggcccgttc
     2761 cacctcggcc gctttgccct gtcgcagcag caacgccagc cagttgtacc gcgcttccca
     2821 atagtgttcc ggcgagcgct gccgcaggcc ggaatccttc agcaggaccg cccaagcctc
     2881 ttgagcggct ttgagttgct cggaagcggc tctgagctgc gcggaatcgg cggctttggg
     2941 ttgctcggaa tcggttttga attgtgcgga atcggctctc ggctgctcgg aatcggcttt
     3001 gggttggtcg gaatcggctc tgagttccgc ggcatccgcg cccgaaccgg ccggcggaag
     3061 ctgcgccgtc agcaccagcg cgagcagacg ctgtgcgtgt ccgttatgag cgtcattcgc
     3121 gaggatctca accagccgct cgcgcgcttg ctccagttcg ccggccgtgt acagcatctg
     3181 cgcgcgggcc agagtcgccg gcgcgacggc cgcggcccgc gacggctcct gccgcaccca
     3241 tctctcaagc tgctcgaagg ccggcaacga ctcaagcgcc agccgccgcg ccgcgtcgaa
     3301 ctcgccctcc aacgtcaggc gctccacttc ctcctgcatc ccgcgcgtca gcaggttcag
     3361 caccggcccg ctctgctcgg cggaaacggc ctccaggtac cgccccagca cacgcgccgc
     3421 ctcgtcgaag cgcttcaagc cgcgcagggc gcggatgcgc accgccaaca cgcggcccac
     3481 gcgctcgtca tgggggtatt gctcgtcgaa cctctcgagc gccgccagcg ccgcctcatc
     3541 atcttgcccc accagcaatt ccgccgcact cacccgcgct tcagccgacc aactccgcgc
     3601 ctgctgcgca tcgcccgccc cggccgccgc acgctgcaac gcctggtcgg cgtaagcggt
     3661 caactcgtcc gccagtgcgc gggcctgcct ctggaactcc gctccggaaa gctctgcccg
     3721 ccgctgctcc agggccaatc gccggcagac gcgcagccgg ccctgcgcct cttcccaata
     3781 ctgcgaatcg cgccccacct tggcaaactc atccgccgcc gccgcataac gccccgctgc
     3841 ctgccaagcc accggcaaca gccacgcagc ccgttgtcgc tgcgggtgat cggcgaaact
     3901 ttgaatcagg ttcaccagcg tctcagccag ccgttcataa gcggaccggc ttcggctgct
     3961 ctgggcgacc tcggcccaga gctggtaggc gaaagtcgcc gcctgcggag ccaatcggtg
     4021 actgcgatac tcgcgcgaca gcacgtcaaa cgtctcggcc gcgtcgaccg gccggtccaa
     4081 gcggtaatag cacttggcca attcgaacaa aagctggcct gcaagttcgc gtgactctga
     4141 gcttacgcca ggcgcggcgc cgcgtggctc atcggttccg gatttttgca gcgcgaactc
     4201 gggccgtccc agcgccgcct ccagccgttg ccgggcttcc tccaactgcc cggcctccgc
     4261 aaagtggcgc gccgcgtaga gcaactcgcc cggtgagagc cgttgcggat cggcgtttaa
     4321 gtcgatgctc gctcctatgt agagctgaac cagcgcgggc caaggaccgc ctcgggccgc
     4381 catcacctcg aatttccgca acgcgatctc gcgcgcctgc tgggcctgct ggcgcagagc
     4441 tggatcgccg ccggcgccgc cagcctccgc ttccagagcc cgtgcttcca gcagatagct
     4501 gtttgcgtcc catagacgag ccaggttgat gtaaaactgc accacctgct gctccggcga
     4561 gggctcggcc gtcgccaatt ccagtagcgg ccgcagcagg gcgcgcgcct cggcgaattt
     4621 gccgctggcc agatgacagc gcgcccactc atacctgatt ctcgccgcct ccaaaggggc
     4681 agactcggcc ttgagcgccg cggcgaaatt ccgctcggcc tcgccaagac gccccaattc
     4741 gcgctgcgcc agcgccagcc ccaacaagca gcggccccgc gcctgcttgg cccggccagc
     4801 ctctagcagc atccgaaagg cgttctcggc gtcactcagc gcttcgagcc gcgcctcctg
     4861 attgcgttcg tccaacaccg cgcagtagta gccggcccag gcgtcattga attccgcatc
     4921 gaacttcgtt tgcacgatgc tgtcgtagat gccggccgcc agaaattcgt cctcacgctg
     4981 ccgcaacgcg ctgtggaccg gctcgatcag cagctccgcg cgccggcact gaatgcgcgc
     5041 ggtccccagc cacgccagca gcttcgcccg atcgccctgt tgccctgccg taatctcaaa
     5101 gcggtccaac tcgccggccg cttgcaggtt catgaccatg ctcgcgtatt ccaggcgcgc
     5161 cgccgcctgt tcgagtaagg tctgcggcga cgtatcaccc ttagcggcct ggtcaacggc
     5221 cgcaatccag cgctcgtacc actcggccga tcgttggcaa gcttgggaac gctcggtcgc
     5281 gtcagccacg cgccgcgcca ggttggcgta ggcctgcgcc agcagacggc attcttctgg
     5341 ccggctcgtc tccccgaacc gcgccagaac ctccggccgc ccctcttgca ccgcctgaag
     5401 gaggtcagcc ggcaattccg tcggctccgc cccagcactg ctgctcacgc agagtccaac
     5461 aagcagcagg acgcgcaggg acttgaagcc caaaactcga aactcgaaac cgctcatcgc
     5521 tcagccgcat cctccggccg gaagctgact gcgaactgca cattcttgaa gccggcctgc
     5581 acggcagcgt ccatggctga gacgacgtat cgccagcgga cggtcgtatc cgctcctata
     5641 accaccatgt tctcgggg
//
LOCUS       DAOSYS010000235         5649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12013_length_5649_cov_5.540222, whole genome shotgun sequence.
ACCESSION   DAOSYS010000235 DAOSYS010000000
VERSION     DAOSYS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12013_length_5649_cov_5.540222"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1203
                     /locus_tag="PLU99_12765"
     CDS             160..1203
                     /locus_tag="PLU99_12765"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOO17964.1"
                     /translation="MFGAAKTASVILVAALDTRAAVQAIDLGVIICGGLCALGVVFWL
                     IRRGEWRNPLAGVRAAALWRVTPAPLAGFPVQPEAGRQPEVDQSAEPGRAEIPSLPAA
                     RLTLGPQGPTLLHVAIALGAYAIVLQLAVTAFLAGQDLQAATQPGMRAWFLVQAADTL
                     TKLLVSLLVVFMLARHPLFPRDAALPRIIRKAGIGLLTGLILAPITLVQLQMGSVIWQ
                     WLHPAQTLPVHPVLLALKESTVEAWGPAVLAIGAILVAPLGEELLFRGLVLGAVWRLT
                     GRAWLSVVISGVLFGAIHGQPQDILPLCTMGVILGYVRLRTRSLVPPITAHALFNART
                     MIMALLFPETIGS"
     gene            complement(1249..2184)
                     /locus_tag="PLU99_12770"
     CDS             complement(1249..2184)
                     /locus_tag="PLU99_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOO17965.1"
                     /translation="MGRREAAARPACHHPTRVRSWNCLSIDVEEYFHCEAFAGAIRPQ
                     QWPLFERRAEPRLELIADLLERYESRATFFVLGWLVPHLTRLLRELAARGHEIACHGY
                     GHQHLARMTPAALADDLRRAQDVIGNCLGVSPRGYRAPTFSVTRRTAWALDVIAAAGF
                     EYDASIFPVRHDRYGVPDAPLTPFHAVGPGGTRVLEFPPLTMQCCGVRIPLAGGGYMR
                     LLPGWTLRAAVRSRMARGGPVMLYLHPWELDSDQPELPAGRLGRWRHRVNLHTTAAKL
                     EALLKRFTFETAGDILRDYVSRRGELQQYFLAAPA"
     gene            complement(2190..3392)
                     /locus_tag="PLU99_12775"
     CDS             complement(2190..3392)
                     /locus_tag="PLU99_12775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO17966.1"
                     /translation="MIDNRNIVCIASSWFDHPTSKHHVMRILAERNRVIWVNYHASRR
                     PNLSIRDFRLALRRLRRSCAGPRRVPAGLTVLSPLLAPFPQSRLARAYNTRVMVRQIG
                     KALAALPAARRQLWLFAPDTPELIRHFDFERVVYYCVDDFAEFSGYDPSAVQRLEAAT
                     LAVSDVVIATSTQLFEGRRAVHSNTHLVPHGVDFEHFAPAAEPGRFEIPPEMRHMPRP
                     VLGYVGLISDYVDLELLAQAARARPAWSFVLVGNVNCALGALSGLPNVHLLGGRPYED
                     LPRYFAAFDVGLIPFRMNRLVRAVNPIKLREYLAAGLPVVSAPLEAVLGYAPAVYPAQ
                     NLEQFLAACDMALRENHPAARRARQNLVRAESWHCRVERISEIVSGGAARQAWEDRIP
                     ALPLGVGR"
     gene            complement(3389..4603)
                     /locus_tag="PLU99_12780"
     CDS             complement(3389..4603)
                     /locus_tag="PLU99_12780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO17967.1"
                     /translation="MRILMLLPRFPYPPERGDTLRSWTVLQGLSGRHEVWLACVDSRM
                     PTREQLAPVREVCREVAVFARSAAARVLGGVRSVLAGGSFTEGYFADWRLRRTLAEWS
                     AREPFDALFTYSSSIAPLAREVRARRRVMDLCDVDSQKWTRYSREAPPPLRWCYRLEA
                     ERVADLEVWAAGRHDVCLTVNERECKKLHLLAPQAVVEVVPTPVKVEPTGNGPPPTAP
                     VIGMLGSMCYPPNVLAVDWFGRFVWPLVQRRLPEARWLIVGRDPVRSVRKWNRLPGVT
                     VTGRVPDVCPYLAQMRVFVDPVRGDIGVQTKLLVALANGRPAVTTSDAVAGLTYSGQP
                     PCLVANTPADFASAVVRLTLDAELWHRLSRRGRELIEQKHASQVETVEQLLTAGRPAA
                     VRRGSGRALVTA"
     gene            complement(4615..>5649)
                     /locus_tag="PLU99_12785"
     CDS             complement(4615..>5649)
                     /locus_tag="PLU99_12785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO17968.1"
                     /translation="PVVIEQAEAAPGAGAWARYVCEARGGTVFHDPAWCAAVERTFGH
                     RPLHRVALRGGKLVGVLPLMEMRSLLAGRMLVSVPYGNYGGILGDDECVISALAEEGE
                     LLAQQRAARVLEVRSIEAVAPGWETVDRYETFERRLPASVAEWECFLPQHARAAARQA
                     RENVEIRHDPQQLPELWQLYSRSMRRLGSLNYPLRFFTELQRLFGKCAWISMAYQGRR
                     PIAGVFSLVWGDTVTPYIQGVDERIRVRGAVNLLYLSIMERAVSCGLRRFDFGRTRKG
                     NVGAAAFKRNQGFRPRPLGYQRYVPPGQRAPDLAPDSARYQLARRIWRRLPLRMTRTA
                     GAWLARSVAG"
BASE COUNT         1049 a         1978 c         1682 g          940 t
ORIGIN      
        1 ggggctctca accggcccct tgacatcgac cgcttcatag atgccatgcc caagcgccaa
       61 gcgcgggcat gcctaaccga ctcaacctcc cagccttgag acgccgaccg cgttgcaggc
      121 gctcgacttg ctgccggttg tgcggcagaa tgcccggcga tgttcggtgc cgcgaaaacg
      181 gcttcagtca tactggtggc agctctcgac acgcgggctg cggtccaggc gatcgacctc
      241 ggcgtcatta tttgcggggg actttgcgca ctcggagtcg tgttctggct gatccgccgg
      301 ggtgagtggc gcaacccgct ggccggcgtg cgggccgccg ctttgtggcg cgtcacccct
      361 gcgccattag ccggctttcc ggtgcaacct gaggcaggcc gccaacccga ggttgaccaa
      421 tccgctgaac cggggcgtgc cgaaatcccg agccttcctg cggcacgact gacgcttggc
      481 ccccaaggcc caacgttgct gcacgtcgcc attgcgctgg gggcgtatgc gatcgttttg
      541 caactggcag taacggcgtt tctggcaggg caggatttgc aggccgccac tcagcccggc
      601 atgcgggcct ggttcctggt acaggcggca gacacgctga ccaagctgct cgtcagcctc
      661 ctggtcgttt tcatgcttgc ccgccatccg cttttcccga gagatgcggc cctcccgcga
      721 ataatccgga aagccggaat cggactgctg accgggctga ttctggcccc gatcaccctg
      781 gtgcagttgc agatgggctc cgtcatttgg cagtggttgc atcccgcgca aaccctgccg
      841 gtccacccgg tgctgctcgc gctcaaggag agcacggtgg aagcctgggg accggcggtc
      901 ctggcgatcg gcgccatcct ggtggcaccg ctgggcgagg aactcctgtt ccgcggattg
      961 gtcctgggcg ccgtctggcg cctgactggc cgcgcctggc tttccgtggt tatctccggc
     1021 gtcttgtttg gggcgattca cggccagccg caggatattc tgccgctgtg taccatgggc
     1081 gtcatcttgg ggtacgtgcg gctgcggacg cgctcactcg tgccgccgat caccgcgcac
     1141 gcacttttca acgcacgaac gatgattatg gcgctcctct ttccggaaac gatcggcagc
     1201 taaatcggct ccctattcct gcaagacgcc cggtaacgaa atcgctcttc aagcgggagc
     1261 cgcaaggaaa tactgttgca attccccacg ccggctcacg taatcacgca gaatgtcgcc
     1321 ggccgtctca aaagtgaacc ttttcagcag cgcttccagc ttggctgccg tggtgtgcaa
     1381 atttacccgg tgtctccagc ggcccaatcg cccggccggc aattctggtt gatcagaatc
     1441 cagctcccac gggtgcagat agagcattac cggcccgccg cgcgccatgc ggctgcgcac
     1501 ggccgcacgc agtgtccagc caggcagcag acgcatgtaa ccgccgccgg ccagtggaat
     1561 gcggacaccg cagcattgca ttgtcaacgg cggaaactcg agcacccgcg taccgcccgg
     1621 acctaccgcg tggaacggtg tcagcggggc gtcgggaacg ccgtagcgat catgccgcac
     1681 ggggaaaatg ctggcgtcgt actcgaagcc ggccgctgca atcacgtcca gcgcccaagc
     1741 ggtccgccgc gtaaccgaaa atgtcggcgc ccggtagccg cgcggcgaca cgcccaggca
     1801 gttgccaatg acgtcctgtg ctcgccgcaa atcatcagcc agagccgccg gcgtcatacg
     1861 ggccaagtgc tgatgcccgt agccgtgaca ggcaatctcg tgcccccgcg ccgccaattc
     1921 gcgcagcaaa cgtgtcaggt gcggcaccaa ccaacccagt acgaaaaaag tagctctaga
     1981 ctcgtaacgt tcgagtaagt ccgcaattag ctccagtcgc ggctccgcgc ggcgctcgaa
     2041 tagtggccac tgctgcggcc ggattgctcc cgcgaacgcc tcgcagtgga agtactcctc
     2101 gacgtctatg ctgaggcaat tccacgacct gacgcgcgtt gggtgatgac atgcaggtcg
     2161 cgcggcagct tcacgtctgc ccacgagtat catcgtccga ccccgagcgg caaagctgga
     2221 attctgtcct cccaagcttg acgtgcggca ccgcccgaaa cgatctctga tatccgctcg
     2281 acccggcagt gccagctctc ggcgcgcacc aggttttgcc gggcgcgtcg cgccgccggg
     2341 tggttttcac ggagggccat gtcgcaggcc gccaggaatt gttctaggtt ctgggccggg
     2401 tacactgccg gggcgtaccc gagcacggct tcgagcgggg ccgaaaccac cggcaagccc
     2461 gccgccaggt attcgcgcaa cttgatcgga ttcactgctc ggaccagccg gttcatgcga
     2521 aaaggtatca agcccacgtc gaacgccgcg aagtaacgcg gcaagtcttc atacgggcgg
     2581 ccgccaagca agtgcacgtt cggcaaccca ctcaacgcgc cgagcgcgca attcacgtta
     2641 ccaaccagca cgaacgacca ggccggccgg gcacgcgccg cctgggccag caattccaaa
     2701 tccacataat cgctgatcag tcccacgtac cccaacaccg gcctgggcat atgccgcatt
     2761 tcaggcggta tctcgaaccg gcccggctcg gctgccggcg cgaaatgctc aaagtcgacg
     2821 ccgtgcggaa ccaaatgcgt attcgaatgc accgcccgcc gtccctcaaa gagttgcgtg
     2881 gaagtggcaa taaccacgtc gcttaccgcc agtgtggcag cttccagcct ttgcacggct
     2941 gacgggtcgt aaccgctgaa ctccgcaaaa tcgtctacgc agtagtagac tacgcgctcg
     3001 aaatcgaagt ggcggatcaa ctccggcgta tccggtgcga aaagccacag ttggcgccgg
     3061 gccgccggta gcgccgccag cgccttgccg atctgccgca ccatcacgcg cgtgttatag
     3121 gcgcgcgcca gccgcgactg cggaaaaggc gccagcaggg gcgataaaac ggtgagcccg
     3181 gccggcacac gccgcgggcc cgcgcaactg cgccgcaaac ggcgtaacgc cagccgaaaa
     3241 tcgcgaatac tcaaatttgg ccggcgcgag gcgtggtaat tgacccagat cacgcggttc
     3301 cgctcagcca gaatgcgcat tacatggtgc ttgctggtgg ggtgatcgaa ccaactgctc
     3361 gcaatgcaga ctatatttcg attgtcaatc atgcggtcac gagcgcgcgc ccgcttccgc
     3421 ggcgcaccgc ggccggtctg ccggctgtca gcaactgctc aaccgtttcc acctgcgaag
     3481 cgtgcttctg ttcgatcaac tcccgcccgc gccggctcaa gcggtgccaa agttcggcat
     3541 cgagcgtcag ccggactacg gccgatgcaa aatcagccgg agtattcgcc accaggcagg
     3601 gcggctgacc gctatatgtc aggcccgcca ctgcgtctga cgtagtcacc gctggccggc
     3661 cgtttgccag tgccaccaac agtttggttt gcacgccaat gtcgccgcgc acgggatcga
     3721 cgaatacccg catctgggcc agataggggc atacgtcagg aacgcggccc gtgaccgtga
     3781 cgcccggcag ccggttccat ttgcgcacgc tgcggacggg atcgcgccca acgatcaacc
     3841 agcgcgcctc cggcagccgg cgctgcacca ggggccacac gaagcgcccg aaccagtcaa
     3901 ccgcgagaac gttcggcggg taacacatcg agcccagcat gccgatcacc ggcgcggttg
     3961 gcggcggacc gttgccggtt ggttcgactt ttaccggcgt tggaacaacc tccaccactg
     4021 cttgcggggc caacaggtgg agcttcttgc attcgcgctc gttcacggtc aggcacacat
     4081 cgtgccgccc cgccgcccac acctccagat ccgccacgcg ctcagcctcc aatcggtaac
     4141 accagcgcaa tggcgggggc gcttcccgtg aatacctggt ccacttctgg ctgtccacgt
     4201 cgcacaagtc catcacgcgg cgccgcgccc gaacctcccg cgcaagcggc gcgatggacg
     4261 aagaatacgt aaacaacgcg tcgaagggtt ctcgtgccga ccactccgcc agcgtgcgcc
     4321 gcagtcgcca gtcggcgaaa tagccttccg tgaaactgcc gcctgccagc acgctccgca
     4381 ccccgcccag cactctggcc gcggccgagc gcgcaaatac cgccacttca cggcaaactt
     4441 cacgcaccgg cgcgagctgt tcgcgggtcg gcatccggct gtccacacac gccagccata
     4501 cctcgtgccg gccgctcagc ccctgcagca ccgtccacga acgcagcgta tcgccccgtt
     4561 cgggcgggta ggggaaccgc ggcaacagca tcagtatgcg cattcttccc cgcttcatcc
     4621 tgcaaccgag cgggccaacc acgcgcccgc cgtccgtgtc atccgcagcg gcaagcgccg
     4681 ccaaatacgc cgcgccaact gataccgggc gctgtcgggc gccaaatcag gggcgcgctg
     4741 cccgggcggc acgtaccgct ggtaacccag cgggcgcggc cgaaagcctt ggttgcgctt
     4801 aaaggccgcc gcacctacgt tgcccttacg cgtgcgcccg aagtcgaagc gccgcagccc
     4861 gcagcttacc gcccgctcca tgatcgatag gtacaacaga ttgaccgccc cgcggacccg
     4921 gatgcgctca tccacacctt gaatgtaggg cgtcaccgtg tcgccccaaa ccaggctgaa
     4981 cacgcccgcg atcggcctcc ggccctgata agccatgctg atccaagcgc acttgccgaa
     5041 cagcctctgc agttcagtga agaagcgcag cggatagttc agtgacccca aacggcgcat
     5101 gctgcgcgaa taaagctgcc acagctccgg taactgctgc ggatcatggc gaatttctac
     5161 gttttcccgc gcctggcgag ccgcggcccg cgcgtgctgt ggcagaaagc attcccactc
     5221 ggccacgctc gcgggcaacc gccgctcaaa cgtctcgtac cggtcaacgg tttcccagcc
     5281 cggcgcgact gcttcaatcg aacgcacctc gagcacgcgc gccgcacgct gctgcgccaa
     5341 aagctcgcct tcttcagcca aggcgctaat gacgcactca tcgtcaccca agatgccgcc
     5401 gtagttgccg tatggaacgc tgaccagcat gcgcccggcc agcaagctgc gcatttccat
     5461 cagcggcagc accccgacca gtttcccgcc gcgcagcgcc acacggtgca acgggcgatg
     5521 gccgaacgtc cgttcaaccg cggcgcacca ggccgggtcg tgaaaaacag tgccgccccg
     5581 cgcctcacac acgtaccggg cccatgcgcc ggctcctggc gcggcctcag cttgctctat
     5641 gaccacggg
//
LOCUS       DAOSYS010000236         5633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12064_length_5633_cov_4.906597, whole genome shotgun sequence.
ACCESSION   DAOSYS010000236 DAOSYS010000000
VERSION     DAOSYS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_5633_cov_4.906597"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="PLU99_12790"
     CDS             complement(<1..1922)
                     /locus_tag="PLU99_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17969.1"
                     /translation="MEVAEHFYTDDPAQGPPDAKTTLDGVDYFFLGNGLIQAAVQVCT
                     SGQGTPVGLLVLNPERLGPKRRALTFDAQHGLATTMMRVRAGNGLSNPRGESLDARWT
                     DIDGVPAVRVEWQGDGLCVEEVFYCPDRRTPRLVRVIRVRTTSAQGLSAVLQTGPEAE
                     PLELPVELASEGYAEAALCYELSKSGDEWRVGVQWSRQTPEHDESAAYWRRCAALTCS
                     AEPLEHLFRAARNQLPAVVAASGMVDASFWQYSLEWVRDQSIIAASLAALGAVDTART
                     MIERLLTQLVSEEGDCVDSGRRRATEEVELDQNGELLLAVESYVNWTGDLDIVRQHWE
                     RVAALAEFPLREVFRHAPSGLLHNRREYWERHVVHGIRDGMELTHQLYAALGLAAAAR
                     LAHMVSQPEQAERWAREAQRIREAMLTDARFGLVENGRFIKRREVSGAVQETITPAAN
                     AGLPAGVPLFGEGTHYLNPDTCAALPIALEFVDPRGELARNTLADMEQLWNQRWDGGG
                     YGRYHVSSEPDSPGPWPFASLFVARAYLEAGEDDKVWRVLNWLGSAPGARAGAWFEFY
                     GPRPIPPYPQVGIIPWTWAELACFFVHHLLGVRPGLAELVLRPRLLSGLESVEARLRL
                     REHNLNLTVRRPRGGET"
     gene            complement(1965..2600)
                     /locus_tag="PLU99_12795"
     CDS             complement(1965..2600)
                     /locus_tag="PLU99_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOO17970.1"
                     /translation="MTVLDEIAQAVIDGDEAHAPQLVEQALAAQTPARAILNEGLLKG
                     MTEVGRRFREGEYFIPEVLIAAEAMKAGSNALKPHLVAAGVKPEATAVIGTVRGDLHD
                     IGKNLVATMLEGAGFEVVDLGVDVAPEQFVAACRQRRVDVVAMSALLTTTMPMMEESI
                     KKLRAELQTAPAILVGGAPVTREYADKIGANGYGKDAATGAEVAKRLALQQ"
     gene            2888..3673
                     /locus_tag="PLU99_12800"
     CDS             2888..3673
                     /locus_tag="PLU99_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOO17971.1"
                     /translation="MAQPAAAQVPTVTPVEAALHFRVLHLDRVEPDRELIHKLAHIQP
                     DRPAHARWVEQVEQLTQELRPLTRPRALFRIDPIVSLEDRRVVLASGAAFEGAVGTFL
                     KNATLIATFVVTIGSAVERLSRIWLRAGKIMQGTIADAIASQCAEATCQTLCRHVRLW
                     AQERACETTPPYSPGYCGMSVRQQIPLFASLPTERINVHRTPSCLMLPVKSISGLIGI
                     GPPDKINPQKHPCEACAHPHCTQRRAPCNHELAANETTQATGD"
     gene            3675..>5633
                     /locus_tag="PLU99_12805"
     CDS             3675..>5633
                     /locus_tag="PLU99_12805"
                     /inference="COORDINATES: protein motif:HMM:NF020120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOO17972.1"
                     /translation="MPDPTPVVAVLLLSATMTNRDGAPTSPAPQQPGPPQHLRCEYLT
                     DPLGIDRTSPRLSWEMVDSRRGAMQTAYQILVADAPELLAADRGNLWDSGQVRSDQSV
                     HVSYAGLPLKSRMQAWWKVRVWDKDGQPSPWSEPARWSMGLLNPTDWHASWIGDSAPL
                     PSSEPPDPHPALMLRKTFQVPAPQQQPAPTPVKRATIYATALGLYELRLNGQRVGDHV
                     LAPEWTDYHKRVQYQTCDVTHLVRPGENALAALLGDGWYCGRIGLAFAVPGGQTRAFF
                     GRQPWLLAQLEIELADGHTITVVSNDEWRVTNEGPIRASDILDGEGYDARRELPGWDA
                     PGFDDSAWRPAKVCSLASPQDLGLTTTQPARPKLVAQPNEPIRVIREIKPIALTEPQR
                     GVYVYDLGQNLAGWCRMKLSGPAGATVTLRHAEVLNPDGTIYTANLRGAPQTDQYTLR
                     GPQGDGQGEVEVFEPHFTYHGFRYVEVIGLPEKPAPDALVARVISSAAPVCGRFECSN
                     PLLNKLMENIVWTQRANMPSVPTDCPQRDERLGWMGDILAFSQAGMFNLDMAAFFTKW
                     LPDVRDAQADDGRYPDFAPHPFDPNVRFSAAPAWGDAGVFVPWRAYVNYADQRLLAEH
                     YESACRWIDWVQANNPDLLWKNKRGNDYG"
BASE COUNT         1011 a         2098 c         1625 g          899 t
ORIGIN      
        1 gtttcgccgc cgcgcgggcg ccgcacagtc aaattcaaat tgtgctcgcg cagccgcaat
       61 cgagcttcca ccgactccag tccgctcaat agccgcggcc gcagcacgag ctccgccagt
      121 cccggccgca cgccgagcaa atggtgcaca aagaagcacg ccagctcggc ccaggtccag
      181 ggaataatgc cgacttgcgg atacggcggg atcggccgcg gcccgtagaa ctcaaaccag
      241 gccccggccc gcgcaccggg cgcgctcccc agccagttga gaacacgcca gaccttgtca
      301 tcctcgcccg cctccaggta cgcccgggcg acaaacaacg aagcaaacgg ccacggcccc
      361 ggcgaatccg gctcagaact gacgtggtag cgcccgtaac ccccgccgtc ccagcgttgg
      421 ttccacagtt gctccatatc cgccagcgta ttccgcgcca gctccccgcg tggatcgacg
      481 aactccagcg caatcggcaa cgccgcgcaa gtgtccggat tcagataatg cgttccctcg
      541 ccaaacagcg gcacaccggc cggcagcccc gcgttcgcgg ccggggtgat cgtctcctgc
      601 accgccccgc tcacctcacg ccgtttgatg aagcggccgt tctcaaccaa accgaagcgc
      661 gcatccgtga gcatggcctc gcgaatccgc tgggcctcac gcgcccaccg ctcggcctgc
      721 tccggttgac tgaccatgtg ggccaggcgc gcggcggccg ccagacccag cgcggcgtag
      781 agctggtgcg tcaactccat cccgtcgcga atcccgtgaa ctacatgccg ttcccagtac
      841 tcccgccggt tatgcagcag gccggaaggc gcgtgccgga aaacctcgcg cagcggaaac
      901 tccgccaagg ccgcgacgcg ctcccagtgt tgacggacga tatccaggtc gccggtccag
      961 ttgacgtacg attccaccgc cagcaacagc tcgccgttct ggtcgagttc cacctcctcc
     1021 gtcgcccgcc gccgtccgga atcaacgcaa tcaccctctt cgctgacaag ctgcgtcagc
     1081 agccgctcga tcatggtgcg cgcagtatca accgcgccca gtgcggccag actggcggca
     1141 atgatcgatt gatcgcgtac ccattccagg ctgtattgcc aaaagctcgc atccaccatg
     1201 ccgctcgcgg ccaccaccgc cggcaattgg ttacgcgcag cacgaaacag gtgctctagc
     1261 ggctccgccg agcaagtcag ggccgcgcag cgacgccagt atgcggcgct ctcatcatgc
     1321 tccggcgtct gcctcgacca ctgaacgccc acccgccact catcgccgct cttactcaac
     1381 tcgtaacaca gcgccgcctc tgcgtagccc tcgctcgcca gttcgaccgg caactccaac
     1441 ggctccgcct ccggcccggt ttgcagcacc gcactcaaac cctgcgcaga cgtagttcga
     1501 actcgtatga cacgaaccag ccgcggcgtc cgccggtccg gacagtagaa gacctcctcg
     1561 acgcacagac cgtcgccctg ccactcgacg cgcactgccg gcacaccgtc gatatccgtc
     1621 cagcgcgcgt ccagcgactc cccgcggggg ttcgacagtc cattccccgc gcgaacacgc
     1681 atcatcgtcg ttgccaggcc gtgctgggca tcaaaagtca gcgcgcggcg cttcggccca
     1741 agccgctccg ggttcagcac cagcaggccg actggcgtgc cctgacccga tgtgcacacc
     1801 tgcaccgcgg cctggatcag gccattgccc aggaagaagt agtccacgcc gtccagcgtg
     1861 gtcttcgcat ccggcggccc ctgcgcagga tcatccgtat agaaatgctc ggcaacttcc
     1921 atgcgctcct cctcacgatg gcgttcagcc gccgtgtcta accactattg ttgcaacgcc
     1981 aacctcttcg cgacttccgc cccggtggcg gcgtcctttc cgtacccatt ggcgccgatc
     2041 ttgtccgcgt attcccgcgt caccggcgcg ccgcccacga ggatggccgg cgccgtttgc
     2101 agttccgccc gcagcttctt aatggactcc tccatcatcg gcatggtcgt ggtcaacagc
     2161 gcgctcatcg ccaccacgtc cacccggcgc tgccggcagg ccgccacgaa ctgctccggc
     2221 gcgacgtcca cgcccaggtc cactacctcg aagccggccc cctccagcat cgtcgccacc
     2281 agattcttgc cgatatcgtg caggtcgccg cgcaccgtgc cgatgaccgc cgtcgcctca
     2341 ggtttcacac cggccgcgac cagatgcggc ttgagcgcgt ttgatcccgc tttcatggcc
     2401 tcggcggcga tcagcacttc ggggatgaaa tactcaccct cgcgaaaccg ccgcccgacc
     2461 tcggtcatcc ccttaagcag gccctcgtta agaatggccc gcgccggcgt ctgcgccgca
     2521 agggcctgct cgacaagttg tggcgcgtgt gcctcgtcgc cgtcgataac tgcctgcgcg
     2581 atctcgtcca gtaccgtcat ggtttgctcc aaggggcatg cccgcgctca gcgcttgggc
     2641 atggcaccca agggcttacc gcttgggcat gacgccccag gcgggtttgc gaaggcggac
     2701 cgccagtata gcgcgccgtg caacagaggc aactgagccg cactggttgc ttcgccgcgg
     2761 gcgcgcggcg gcgcggcgca ggcgcgtggc ggagtcggtg catggcggcg cggcgcaatg
     2821 cggcgcaggc gcgggtatga tgtgctaacg tccaaagcgg ccgcggagga gtgacagagg
     2881 agtgacagtg gcacagccgg cagcagcaca ggtccccacg gtgacacccg tcgaggccgc
     2941 gctccatttc cgcgtccttc accttgaccg cgttgaaccc gaccgcgaat tgatccacaa
     3001 gctggcccac attcagcccg accgccccgc gcacgcccgt tgggtcgagc aggtcgagca
     3061 gcttacccag gagctgcgac ccctcacccg cccgcgagca ctcttccgca tcgaccccat
     3121 cgtctcgctc gaagatcgcc gcgtggttct agcatctggc gcggcattcg agggcgccgt
     3181 cgggacgttt ctcaagaatg cgaccttgat cgccacgttc gtagtgacca tcggctccgc
     3241 agtcgagcgc ctcagccgaa tctggctcag ggcaggcaaa atcatgcagg gcacgatcgc
     3301 ggatgcgatc gcctcgcagt gcgccgaggc cacctgccaa acgctctgcc gccacgtgcg
     3361 cctctgggcc caggagcgcg cgtgcgagac aacgcccccg tacagccccg gctattgcgg
     3421 catgagcgtg cgtcagcaaa tcccgctgtt tgccagtctg ccgaccgagc gaatcaacgt
     3481 ccaccgcacg ccaagctgcc tcatgcttcc ggtaaagtcc atctccggcc tgatcggcat
     3541 cggcccgcct gacaagatca acccgcagaa gcacccgtgc gaagcctgcg cacaccccca
     3601 ctgcacgcag cgccgcgcac cctgtaacca cgaactggcc gccaacgaaa cgacccaagc
     3661 aacaggtgat tagcatgccc gacccaacac cggtcgtcgc agttttgctt ctttcggcca
     3721 cgatgacgaa tcgcgatggc gccccaacgt cgccggcccc acagcagccc ggcccgccgc
     3781 aacatctgcg ctgcgagtac ctgaccgacc cgctgggcat cgatcgcacc agcccacgcc
     3841 tatcgtggga gatggtggat tcccgccgcg gggcgatgca gactgcttac cagattctcg
     3901 tcgccgatgc gccggaactg ctcgccgccg atcgcggcaa cctctgggac agcggccaag
     3961 tccgctcgga ccagtcagtt cacgtcagtt atgccggcct gccgctcaaa tcgcgcatgc
     4021 aggcctggtg gaaggtgcgc gtatgggaca aggacggaca gccgtcaccc tggagcgagc
     4081 cggcccgctg gtccatgggg ctgcttaacc ccacagactg gcatgcgagt tggatcggtg
     4141 acagcgcccc cctgccgtcg tccgaaccgc cggacccgca ccccgcgctg atgctccgca
     4201 agaccttcca agtccccgcg ccgcagcaac aacccgcccc tacgcccgtt aagcgcgcca
     4261 caatctacgc cacggcgctg ggcctttacg aactgcgtct gaacggccag cgcgtcggcg
     4321 accatgtcct cgcacccgag tggaccgact accacaaacg cgtgcaatac cagacctgcg
     4381 atgtcactca cctggtccgc cccggggaaa acgcgctcgc cgccctgctc ggcgacggct
     4441 ggtattgcgg acgcatcggt ctggctttcg ccgtccccgg cggacaaacc cgcgcgttct
     4501 ttggccggca gccctggctg ctggcgcaac tggagatcga actcgccgac ggccacacca
     4561 tcacggtcgt ttccaatgac gagtggcgcg tcacgaacga aggccccatt cgcgccagcg
     4621 acattttgga cggcgaaggc tacgacgctc gccgcgaact gccgggctgg gatgcgccgg
     4681 gcttcgacga ttccgcctgg cgacccgcga aagtttgcag ccttgcttcg ccgcaagatt
     4741 tgggattgac cacgacgcag cccgcccgcc caaagctggt ggctcagccc aacgagccaa
     4801 ttcgcgtcat tcgcgagatc aaacccattg cgctgaccga gccgcagcgc ggcgtttacg
     4861 tatacgacct gggccagaac ctggccggtt ggtgtcgaat gaagctgagc ggcccagccg
     4921 gcgcaaccgt caccctgcgc catgccgaag tgctgaaccc cgacggcaca atctacaccg
     4981 ccaacttgcg cggggctccg cagacagacc agtacacgtt gcgcggccca cagggcgacg
     5041 ggcaaggcga agtcgaagtc ttcgagccgc acttcaccta tcacggcttc cgctacgtcg
     5101 aagtaatcgg cctgcccgag aagcccgccc cggacgcact ggtggcgcgc gtaatctctt
     5161 ccgcggcccc tgtctgcggc cgcttcgaat gctcgaaccc actgcttaac aagctgatgg
     5221 aaaacatcgt ctggacgcag cgggccaaca tgcccagcgt gccgaccgac tgcccgcaac
     5281 gcgatgaacg cctcggctgg atgggcgaca tcctcgcatt ttctcaggcc ggaatgttca
     5341 acctcgacat ggccgccttc tttactaagt ggctgcccga cgtgcgcgac gctcaggccg
     5401 acgatggccg ctacccggat tttgcacctc acccgttcga ccccaacgtg cgcttctcgg
     5461 ccgcgccggc ctggggtgac gccggtgtgt tcgtgccctg gcgggcgtac gtcaactacg
     5521 ccgaccagcg cctgctcgcc gagcattacg agtccgcctg ccgctggatt gactgggtcc
     5581 aggccaacaa ccccgacctg ctctggaaga acaaacgcgg caatgactac ggc
//
LOCUS       DAOSYS010000237         5584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12198_length_5584_cov_5.710074, whole genome shotgun sequence.
ACCESSION   DAOSYS010000237 DAOSYS010000000
VERSION     DAOSYS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12198_length_5584_cov_5.710074"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..423
                     /locus_tag="PLU99_12810"
     CDS             13..423
                     /locus_tag="PLU99_12810"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOO17973.1"
                     /translation="MPPEITEIRLWLTKAKRELRASGMALQTADPMTDVAGYHAQQAI
                     EKLLKAHIVHGGGELEKVHDLRLLAISCTALDSSFSPWIERLAPLSAFAARYRYPGPA
                     DPTIEEAPGALAIAEELWQFVTARLPAEAIPPSA"
     gene            566..919
                     /locus_tag="PLU99_12815"
     CDS             566..919
                     /locus_tag="PLU99_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17974.1"
                     /translation="MCLRLVKTIYVRWLAVGITAVAPLATTHFAKGPAPVGDQSESVS
                     WADAHGAAAIPLVERPRAQSSGDHAQRPDGHTQPALRDYCAPAEAAAARITVALRTSI
                     AAPIPTSAFLCVLLI"
     gene            1220..2122
                     /locus_tag="PLU99_12820"
     CDS             1220..2122
                     /locus_tag="PLU99_12820"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOO17975.1"
                     /translation="MTVSDKGAAHPKHRNRLLATLRALIRTRLTTGVVTILPLLVTFW
                     VIRFVFEWMRDASGWALKGLLLSPGGRPYLEHLHFNFERWNRLIEMGLPHAQEQFFEM
                     MPWHVRWGIGAVAVLLTVLVLYAVGIFAANLIGRRVIDFIEQWVDRVPFVKTVYRGLK
                     QVLDSFTGEATQKFQRVAMFPFLTPGVYSLGFVTNIFADPDTGEEYATVFYATTPNPT
                     TGFVFMLRRNDIVELDWTFEETIKAIMSGGILIPDRAQMPKLLQALAAKTKAAPPAVL
                     PPGLGRFEPLAPASFDPGLSPERD"
     gene            2290..2670
                     /locus_tag="PLU99_12825"
     CDS             2290..2670
                     /locus_tag="PLU99_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOO17976.1"
                     /translation="MSDPYYHEPASELSTQVRDEHRALATLIEELEAVDWYHQRIDVA
                     TDADLKAILIHLRDEEIEHSAMCLEWLRRRIGTFDTYLRKFMFKAEPVAELAAASEGD
                     GAARANEPRGRAVGLGLGKLGKEG"
     gene            2674..3477
                     /locus_tag="PLU99_12830"
     CDS             2674..3477
                     /locus_tag="PLU99_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOO17977.1"
                     /translation="MDILRRELAPVADEAWKYLDEQAAQVLKTQLSARTLVDFDGPHG
                     ITLGAVNLGRLDISATDAGGEVPWGVRRVLPLVELRIPFFMDQLEIDNVSRGARDIAD
                     EPLREAARKLALLEERAIYLGFPEGRIEGIIPRAAHEPLTLPGNADDYPRIVADAVNA
                     LHAAGVGGPYWLVLGTQPFYALRSVGATGYPAARIVRQLIDGDILWSPALDGGLLLSK
                     RGGDFEMTVGQDIVIGYAHHDRYKVELYLTESFTFRVLEPLAAVALRAG"
     gene            3674..4447
                     /locus_tag="PLU99_12835"
     CDS             3674..4447
                     /locus_tag="PLU99_12835"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOO17978.1"
                     /translation="MYWGRHEDEGLARREQDRGPGQSDLGPDWRSEHRGRQWRRDPRN
                     WGREGRGPGEPSHDWLREARQWWSAMGAGERDWQREEQRRERAEREIWQAGPYAGQVH
                     EYRERYWRSPGETPIEYVRPAGRQRWAYGAGGGWYEPRGLRHRERGEELGGAWAGWTR
                     EQGPEYRAPKGYKRSDKRIMEDVCDAFMMDERLDPREIEVEVLDGVVILRGSVRHRHE
                     KYLAEDIADSILGVKDVDNQVRIAQPGEVPHVARPTVSG"
     gene            complement(4524..4596)
                     /locus_tag="PLU99_12840"
     tRNA            complement(4524..4596)
                     /locus_tag="PLU99_12840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4561..4563),aa:Lys,seq:ctt)
     gene            4753..5217
                     /locus_tag="PLU99_12845"
     CDS             4753..5217
                     /locus_tag="PLU99_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17979.1"
                     /translation="MPELLEALRARGVVLDRERHGRVTIYEEALRACAAGGVLRSLQI
                     ASPFLARAGCRSRAGLRTVEPPVESVVGKLVPEVRRLGKRVVLALEEELFLALHLMIA
                     VRFRWLPPDRRRPGKVALAVLGFESGTLVLTEAGSKKRAALHIRGAKRRWHK"
     gene            complement(5307..>5584)
                     /locus_tag="PLU99_12850"
     CDS             complement(5307..>5584)
                     /locus_tag="PLU99_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17980.1"
                     /translation="NSGCQVFAAECSGDADDMFLDPLSRRLYVIGGDGQVDVFDCAQN
                     GCVKSDSLPTAPGARTGLLVPEQRLLYVAVPSCRGHSAEIREYALTD"
BASE COUNT         1015 a         1659 c         1884 g         1026 t
ORIGIN      
        1 ggaaagtaat ccatgccgcc tgaaatcacc gagatcaggc tctggttgac gaaggccaaa
       61 cgagagctgc gcgcttccgg catggcactg caaacagcgg acccgatgac ggacgtggct
      121 ggttaccacg cgcagcaggc gattgagaag ttgttgaaag cgcatattgt ccacggcggc
      181 ggcgagttgg aaaaagtgca cgatctgcgg cttctggcga tttcgtgcac ggcgctggac
      241 tcttcgtttt cgccgtggat cgagcgtttg gcaccgttgt cggcgtttgc cgcgcggtat
      301 cgctacccgg gcccagccga cccaaccatc gaggaagcgc cgggcgcact ggcgatcgcg
      361 gaagagctct ggcagttcgt gacggctcgc ctgcccgctg aagcgatacc gccctccgcg
      421 tagcgcggag ctttcctgtc gcgacttcat cagccgcgtt caagagcgcc ctagcttcga
      481 ctctcgcccc gccctccgac ctcgccgtcc gccgtcatca gggcggttgc tttcggcccg
      541 cacgcgggtt actttaattg ctcgaatgtg cctgcgtctc gtaaagacga tctacgtgcg
      601 ttggctggcg gtaggcataa ccgccgtcgc gccgctggcg accacgcact ttgcgaaggg
      661 gcccgcccca gttggcgacc agagcgagag cgtgtcctgg gccgatgcgc acggcgcggc
      721 agccatcccg ctcgtcgaac gccccagggc acagagcagc ggcgaccacg cccaacgccc
      781 cgatggccac acgcagcctg ccctacgtga ttactgcgcg ccggcagagg cagccgcggc
      841 ccgcataacg gtggccctca gaacatccat cgccgcgccc atcccaacct ccgcgttctt
      901 gtgcgtactg ctgatttgat gcagcccttc agctcagctt cgtagtcgtc gtcaaacagt
      961 aaaaccacac ctttcctgaa gggactatgt ctatgaaagg cattgtacgc gctctggctc
     1021 tgatcgttgg aatcatcgct ctgatttgga tctctactat cctcgttaac ttgttcatgg
     1081 gaccagcccc cgtggcccca gcctgaatag gtccggcctc gccgatcacc gccgtagaat
     1141 atctggacca ccgccgggcg cggacgagcg atcgcctgcg cccggccgtg gccgctgagg
     1201 ccgctaacgg agaaactgat tgaccgtttc tgacaaaggc gctgctcacc caaaacaccg
     1261 taaccgtttg ctggccacgc tgcgcgccct gattcgcacg cgcctgacaa ccggcgtggt
     1321 caccatcctg ccgctgctgg tcaccttttg ggtgatccgc ttcgtgttcg aatggatgcg
     1381 cgacgcctcc ggctgggcgc tcaaagggtt gctgctcagc cctggcggcc ggccttactt
     1441 ggaacacctg cacttcaact ttgaacgctg gaaccggctc atcgagatgg gcctgccgca
     1501 cgcgcaagag cagttcttcg aaatgatgcc ttggcacgta cgctggggca ttggcgcagt
     1561 cgcggttctg ctcacggtgc tggtcctgta cgcggtcggc atctttgcgg ccaacttgat
     1621 cggacggcgg gtgatcgact tcattgagca gtgggtcgac cgcgtgccgt tcgtaaaaac
     1681 ggtttatcgc gggctgaagc aggtgctcga cagcttcacc ggcgaggcca cccagaagtt
     1741 ccagcgcgtg gccatgttcc cgtttctgac gccgggcgtg tattcgctcg ggtttgtcac
     1801 gaacatcttt gccgatccgg acacaggcga ggaatacgcc acggttttct atgccaccac
     1861 gccgaacccg accaccggct tcgtgttcat gctgcgccgc aacgacatcg tggagctgga
     1921 ttggaccttc gaggaaacca tcaaagccat catgtcgggc ggcatcctga tccccgatcg
     1981 ggctcaaatg cccaagctcc tgcaagcctt ggcggccaag acgaaggcgg cgccgccggc
     2041 cgtgctgccg ccgggtctcg gccggttcga gccccttgca cccgctagct ttgaccctgg
     2101 actatcgccc gaacgcgact gaaggcacag gcctggctgt agtcctcaat attcgctttc
     2161 gcgcacgctg aaatccacaa tgccgagggc cgggctcgtt tgattttccg gattggcaga
     2221 cataagagtt gatgcgtcgc agcatagtgg ctgtgctgga ggatgagcct tttttccgga
     2281 ggatcagctt tgtcggatcc atattaccac gagccggcgt ctgagttgtc cacgcaagtg
     2341 cgcgatgaac accgcgcact ggccaccttg atcgaagagc tggaagccgt ggactggtac
     2401 caccagcgga tcgacgtagc taccgacgcc gacctcaaag caatcttgat tcatctccgg
     2461 gatgaggaaa tcgaacattc ggccatgtgt ctggaatggc tgcggcgccg gatcgggacg
     2521 tttgacacat atttacgaaa attcatgttc aaagcggagc cggtggccga gcttgcggcg
     2581 gcgagcgagg gtgacggggc ggcgcgggcc aacgagcctc gcgggcgggc ggttggtctg
     2641 gggttgggta agctcgggaa ggaaggttga gcgatggata ttctgcgacg tgagctggcg
     2701 ccggttgcgg atgaagcatg gaagtacttg gacgagcagg cggctcaagt attgaagacg
     2761 cagttgtcgg cgcgaacgct ggtggatttc gacggcccgc atgggattac gttgggggct
     2821 gtgaatctgg ggcggctgga catttcggcg accgatgcgg gcggggaggt cccgtggggc
     2881 gtgcgcaggg ttttgccgct ggtcgagctg cggattccat ttttcatgga ccagttggag
     2941 atcgacaacg tttcgcgcgg ggcccgcgac atcgcggacg aaccgttgcg ggaggcggca
     3001 cggaagctgg cgctgctgga agagcgggcc atttacctcg gattcccgga ggggcgcatc
     3061 gaagggatca tcccccgcgc cgcgcatgaa ccgttgacgc tgcccggcaa cgccgatgac
     3121 tacccgcgta tcgtggcgga cgcggtgaac gcgctgcacg ccgccggggt cggcggccct
     3181 tactggctgg tgctcgggac gcaacccttc tacgcgctgc gctccgtggg tgcgaccggc
     3241 tatcccgcgg cgcgaatcgt gcgccaactg atcgacggag atattctgtg gagcccggcc
     3301 ttggacggcg gcctgctgct ttcgaagcgt ggcggcgatt tcgagatgac cgtggggcag
     3361 gatatcgtga ttggctatgc gcatcacgat cgttacaagg ttgagcttta tttgaccgag
     3421 tcgtttacgt ttcgcgtgct cgaaccgctc gccgcagtcg ctctccgggc gggctaatgc
     3481 gaactggatt tggcgggcgc gccacgcgct ctcgcgaata aggacggctg tcggcccgca
     3541 aacgaggtgg ccgcaggggg gcgcgcccaa acgcgaagaa ccacgcgtta agtgcgaatt
     3601 catcccgtgt taagggggcg ggcctaaatt cgtcgtgggc cgttcgacaa aagacgcgtg
     3661 aagaggtgcg cgcatgtatt ggggcagaca cgaagacgaa ggcctagcac ggcgcgagca
     3721 ggatcgcggt ccgggtcaat cggacttggg gccggactgg cgcagtgagc accgcggccg
     3781 ccagtggcgc cgcgacccac gcaactgggg acgcgaagga aggggccccg gcgaacctag
     3841 tcacgactgg ctgcgtgaag cgcgccaatg gtggtcggcg atgggcgccg gtgaacgcga
     3901 ctggcagcgc gaagagcaac gccgcgagcg ggcggaacgc gaaatttggc aagcgggtcc
     3961 atacgccggg caggtgcacg aataccgcga gcgctactgg cgctcgcccg gtgaaacgcc
     4021 gatcgagtac gtccggccgg caggccgcca gcggtgggcc tacggcgcgg gcggcggctg
     4081 gtatgagccg cgcgggctgc ggcatcgcga gcgcggcgaa gagttgggcg gggcgtgggc
     4141 cggctggacg cgcgagcaag ggcctgagta ccgggcgccg aagggctaca agcgttcgga
     4201 caagcggatc atggaggatg tgtgcgacgc gttcatgatg gacgagcgcc tcgatccgcg
     4261 cgaaattgaa gtcgaggtgc tggatggcgt ggtgattctg cggggctcag tccgccaccg
     4321 gcatgagaag tacttggcgg aggacatcgc cgacagcata ttgggggtga aagatgtcga
     4381 caaccaggtt cgcattgcgc agccgggcga agtgccgcat gtcgcgcgcc cgacagtctc
     4441 cgggtaaacc ggcgcatttt tgggcggcgc aagaaacagg ctggagcgtg ccgaacgcga
     4501 gttcggccgt ccagccttta aattgcctcg gctagggctc gaacctagga cccgctggtt
     4561 aagagccagc tgctctacca actgagctac cgaggcccgt tgggcatccc gccggattgg
     4621 acggaagttc agtattctaa gcggtaacgg gcgcacgagc aagaggccga agccgggaga
     4681 cgacgcgttg cgcggagggg ggcagccaac ccgatacgcc atcggcgccg cgtgggccta
     4741 tgataccgac caatgcccga actactggag gcgctgcggg cgcgaggcgt ggttctggat
     4801 cgagagcggc acggccgagt cacaatctac gaggaggcgc tgagggcgtg cgcggccgga
     4861 ggcgtgctac gcagccttca aatagccagc cccttcttgg cccgcgccgg atgccggagc
     4921 agagccgggc tgcgtacggt tgaaccgccg gtcgagagcg tcgtcggcaa gctcgtaccg
     4981 gaggtgcggc ggctggggaa gcgcgtggtt ctggcgctgg aggaagagct ctttctggcc
     5041 cttcacctga tgatcgcggt ccgtttccgg tggctgcctc ccgaccgtag acggccgggc
     5101 aaggtcgcgc tcgccgttct gggcttcgag agcggaacac tcgtgctgac cgaggctggt
     5161 tcgaagaagc gcgcggcgtt gcacattcgc ggggcgaagc ggcgctggca taagtgataa
     5221 ggtgataagg tgataaaggg cggtctgcgc aatcacttta tcactttatc actccgttgg
     5281 cttcgagcag tgcggtgatg gtaggttcag tccgtcaggg catattcgcg aatttccgct
     5341 gaatgcccgc ggcacgacgg cacagctaca tagagcagtc gttgctctgg gaccagaaga
     5401 ccggtccttg cgccgggtgc ggtcggcagc gagtcgcttt tgacgcagcc attctgggca
     5461 caatcgaaaa cgtccacttg tccgtcgccg ccgattacgt acagccggcg ggaaagcgga
     5521 tcgaggaaca tatcgtctgc atcgccgctg cattcggcgg caaaaacctg gcacccgctg
     5581 ttgg
//
LOCUS       DAOSYS010000238         5523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12372_length_5523_cov_5.550475, whole genome shotgun sequence.
ACCESSION   DAOSYS010000238 DAOSYS010000000
VERSION     DAOSYS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12372_length_5523_cov_5.550475"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1696)
                     /locus_tag="PLU99_12855"
     CDS             complement(176..1696)
                     /locus_tag="PLU99_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17981.1"
                     /translation="MRRTLGLALAMVLLASAAAAHADGRDVLEELGPCMAGAGLPADP
                     ECLAAFDVDGDGDVDLHDFAALQAGVDPVGGCCFDNYSCEILTEAACLAAGGGGEWLG
                     AGSECGQCTLVRIRAITFGGTGSTTIKHDCAYPPCSSYGTQQWLDANHDGDVLDAGDH
                     DYPVAYVRNSHVTLSGVQFWVSPSGTASGVQVRGYGPDGLTFTGTGNLSGSTLNVSGT
                     LVSEGTLPNTVKYYPSFDIVWEVAFDGVNFVDGGTSSTRLYVTRSTPLGDCLESYFHI
                     STQAADGLADEQPVIDAIWAEFADREVYNVDGERLGYYRGVLCASYCTYYSAPELVYY
                     TTSQCGGWADLLRQCFRTQGLSSSVFVTAEPLTSALPLDCGSYPSSAAGFLVKNYTFN
                     GWGTSCTAYPFSFNDPCGYYAAWPSPTCTDATGLPGQDNPNPASWFARHFIVKINMAY
                     YDPSYGAGPFTGTQEEANMQWELGAMAGYFGRAQPSPSRAGVRKDIAQTRETYFDQ"
     gene            2202..3146
                     /locus_tag="PLU99_12860"
     CDS             2202..3146
                     /locus_tag="PLU99_12860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOO17982.1"
                     /translation="MRAVAIDDAGGPEMLTVHQLPVPRIDDHEVLIAVDTAGVGSWDP
                     AIRAGWWPEGRPRFPLVLGTDGAGTIVATGARVRRLKVRDKVYGYSWLSPKGGFYAEF
                     AAVEASKAALIPAGLELREAGAMPATALTALQGIDNALRIKQGDTLIIHGASGGVGTL
                     AVQFAKWRGARVFATASGTDGAELVRDLGADEAIDGRQGDIAAAIRRFAPDGVDAVFA
                     LAGGEPLERCLDALRPDGRLAYPNGVEPEPLKRPGLRVVSYDAVADVGELELLNEAIE
                     ATRLRVVIAEEYPLGQAAKAHERLEAGHVLGKIVLRIR"
     gene            3171..4193
                     /locus_tag="PLU99_12865"
     CDS             3171..4193
                     /locus_tag="PLU99_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO17983.1"
                     /translation="MSPVERVERVLSGRWPDRAPLSFWHHFGPDQVAGEAAVTAHLAH
                     LEAFDLDFLKIMNDNGYPHAGPVERVADLASLTELRGDEGEFGRQLELIAALKRRLRG
                     RVLMTTTVFNAWSTLRHIVRPPREHKPPNLAPDDEVSGQLLSFLAQDEETLRRAIRVA
                     AASLANFARRCLDAGADGIFLSVRDDWLERAGTHRAPYADLVRSADLQILDGAARGRF
                     NMLHVCGTPVHFGDFADYPVHVLNWADRSAGPAIADVRDWVKPAICAGLDNLRTLPEG
                     TPRDCEREMADALHQASDRPILIAPGCTYDPERVPRANLLAVCRAVRASAGAASQMTH
                     TQGRCG"
     gene            4341..>5523
                     /gene="selB"
                     /locus_tag="PLU99_12870"
     CDS             4341..>5523
                     /gene="selB"
                     /locus_tag="PLU99_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOO17984.1"
                     /translation="MSDAPKLVVGTAGHIDHGKSRLVWVLTGTDPDRLPEEKVRGMTI
                     DLGFAHGQFDGCDISFVDVPGHERFIRNMVAGATGIDVAVLVVAADDSVMPQTREHAE
                     VLSLLGVERCIVALSKMDLVDDEWADAVEEEVRGLLTGFGIQPLCCVRTSAEDGRGVE
                     ELRGVLARLAREQSRQAEPYRWFCLPIDRSFVIPGRGTVVTGSVAHGTVSREDELELW
                     PTAERVRVRDLQSHHDARDSAAGRRRLALNLAGVSREDVARGYTLATPGYLEPARFLD
                     VRLATLRMPGKTRKDVLRLRLHIATSEVLAELRLADKPESETVSGVFAQVKTAEPIVA
                     TWGQRFILRDESGMRTLGGGRVLRPVGRLWTARRPIQRQGLQTLFEGQPAARLEEVIR
                     AA"
BASE COUNT         1009 a         1833 c         1720 g          961 t
ORIGIN      
        1 gctgctccgg gtccaccaat gtccactggt tgtaccgcgg aacggcgatt acctgactga
       61 ccgcatccgc cggatcggcc gcgtatgccg cggtccccag tgccaaagcc acgccgatcc
      121 acataccgca aataggcaaa cgatgtacta tcatcggtcc tctccttgcc gaattctatt
      181 ggtcaaagta ggtttcgcgc gtctgggcta tgtccttgcg tacaccggcg cgtgagggcg
      241 aaggctgcgc tctcccaaag tagcccgcca ttgcccccag ctcccactgc atgttcgcct
      301 cctcttgcgt cccggtgaac ggtcccgcgc cgtaagaggg gtcgtaataa gccatattga
      361 tcttcacgat gaaatgccgg gcgaaccaac tggccgggtt ggggttgtcc tggcccggca
      421 agcctgttgc gtcggtgcaa gtcggcgacg gccaggccgc atagtaacca cacgggtcat
      481 taaacgagaa tggataagct gtacacgacg tgccccaacc gttgaaggtg tagttcttga
      541 cgaggaatcc cgcggccgag gaaggatagc tgccgcagtc caacggcagg gctgaggtga
      601 gcggctcggc cgtcacaaac accgaggagc ttagaccctg cgttctgaaa cactgccgca
      661 gtaggtccgc ccagccgccg cactgggaag tggtgtagta caccagctcc ggggcgctat
      721 agtaagtgca gtaagaggcg cacaacacgc cgcgatagta ccccagtcgc tcgccatcaa
      781 cgttgtagac ttcgcgatcg gcgaattcgg cccaaatggc gtctatcacc ggctgttcat
      841 cggccaggcc atcggcggcc tgtgtactaa tgtggaaata gctttccagg cagtctccca
      901 acggcgtgct gcgcgtgaca taaagccgcg tgctgctggt accgccatca acgaagttga
      961 cgccgtcgaa cgctacttcc cagactatgt cgaagctcgg gtaatactta acggtatttg
     1021 gcagggtgcc ttctgaaacc agcgtacccg aaacattaag cgtactgccg gaaaggttgc
     1081 cagtgccggt aaaggtcaaa ccgtcaggcc cgtaaccccg cacctgaacc ccgctggcag
     1141 tgccggaagg cgaaacccag aactgaacgc ccgaaagcgt aacgtgcgaa ttgcgcacat
     1201 aggcgaccgg ataatcatgg tcgcccgcat ccagcacatc gccgtcatgg ttggcgtcca
     1261 accactgttg cgtgccataa ctgctgcacg gcggatacgc acagtcgtgc tttatggtag
     1321 tggagccggt accgccaaaa gtgatcgccc taatgcgtac tagcgtgcac tgaccgcatt
     1381 cgctgcccgc gcccagccat tcgccgcctc cgccggccgc cagacaggcg gcctccgtca
     1441 gaatctcaca cgagtagttg tcgaaacagc agccgcccac aggatcaacc ccggcctgca
     1501 gggccgcgaa gtcgtgcaga tcgacatccc catcgccgtc cacgtcgaac gccgccagac
     1561 attccggatc tgcgggcagc cccgcaccgg ccatgcacgg cccaagctcc tccagaacat
     1621 cgcggccatc cgcgtgcgcc gcggccgcgc tcgcaagcag caccatcgcc agcgccagtc
     1681 cgagtgttct cctcattgtg ctctccaccc tcccgatgta cagtcaacag tcgctcgagc
     1741 agtgcttgcc caagctgcac cactccggtg agactcatcg agccgccgcc ggaatccttc
     1801 gcagattcaa cccagcttaa cacaaacgcc tgtcgatccc aaaccaacct tcgcgcgaaa
     1861 gggctttttt atagccacgc ggccgaacgg ctctctcccc gaaaggtagc cgcaacttcc
     1921 cccattctcg gctgcgcggt gaggtcgctt tgagcggcta gttatcgggc atgccggccc
     1981 cggacgagca cgtgtgagcg caagttcagt cgtggcggca catttcacct gcacccgcgc
     2041 cgccagtggc agtaaatggc cacatggccg gaccggcggc gccggcggca cctacaatca
     2101 gcccggcaaa cgccgaccga ttctgggagt atcaaatggc gccttctcat tccggaaaaa
     2161 ctcctgtaac cccgccggtg ccgcggaccg ttcccttgac tatgcgtgcc gtggcgattg
     2221 atgacgccgg cggtcccgag atgctgaccg tccaccaact gcccgtgcct cgcattgatg
     2281 accacgaggt gctgatcgcc gtcgacactg ccggcgtcgg ctcctgggat cccgccatcc
     2341 gcgccggctg gtggcctgaa ggccgcccac gatttccgct cgtgctcgga acggatggcg
     2401 ccggcaccat cgtcgcaacc ggcgcccgcg tccgccgcct gaaggtccgc gacaaggtct
     2461 acggctacag ttggctcagt cccaaaggcg gcttttacgc cgagttcgcc gccgttgagg
     2521 cgagcaaagc cgcgcttata cccgccggcc tcgagctcag ggaggcgggc gccatgcccg
     2581 cgaccgcttt gaccgcgctg caaggcattg acaatgcatt gcgcatcaag cagggcgaca
     2641 cactcataat ccacggcgct tcaggcggag tcggcacgct ggcggtgcaa ttcgcgaaat
     2701 ggcgcggcgc ccgcgtcttc gccaccgctt cgggcaccga cggcgcggaa ttggtccgcg
     2761 acctgggtgc cgacgaggcc atcgacggcc gccagggcga catcgccgct gcgatccggc
     2821 gcttcgcgcc ggacggcgtc gacgccgtat tcgcgctggc cggcggtgag ccgctcgaac
     2881 gttgcctgga tgccctgcgc cccgacggac gcctggctta tccgaacggc gtggagcctg
     2941 agccgctcaa gcggcccggc ctgcgtgtcg tcagctatga cgccgtggcc gacgtcggtg
     3001 aactcgaact gctgaacgag gccattgagg ctacccggct gcgggtcgtg attgctgaag
     3061 agtacccgct gggccaagcg gccaaggccc acgaacgcct cgaggcggga cacgtgctgg
     3121 gcaaaatcgt gctccgcatc cgctagtgcc gtccgcggcg ggaggccgac atgagtccgg
     3181 tcgaacgagt tgaaagagtc ctcagcggcc gttggccaga ccgcgctccg ctcagtttct
     3241 ggcatcactt cggaccggac caggtcgccg gtgaagcggc agtcacagcg cacctcgcgc
     3301 acctggaggc attcgatctg gactttctca agatcatgaa tgacaacggc tatccgcacg
     3361 ccgggccggt tgagcgcgtg gcggatttgg ccagcctcac agaactccgc ggcgacgagg
     3421 gcgaatttgg ccggcaactc gagctcattg ctgccctgaa acgccggctt cgcgggcgcg
     3481 tgctgatgac gaccaccgtc ttcaacgcat ggtccacact gcggcacata gttcggccgc
     3541 ctcgggagca caagccgccg aacctggcgc cggacgacga agtcagcggg caacttctca
     3601 gttttctggc gcaagacgaa gagacgctgc gccgcgccat ccgcgtggcc gccgccagtc
     3661 tggccaattt cgcccgtcgc tgcctggacg ccggtgcgga tggcattttc ctgtccgtgc
     3721 gtgatgactg gctggagcgc gccggtacac atcgcgcccc gtatgccgac ctcgtccggt
     3781 cggccgacct gcagattctc gacggcgccg cgcgcgggcg tttcaacatg ctgcacgtct
     3841 gcggcacgcc tgtgcacttc ggcgactttg ccgactaccc ggtccacgtc ctgaactggg
     3901 ccgaccgcag cgccggaccc gccatcgctg acgtccgcga ctgggtcaaa ccggctatct
     3961 gcgcgggttt ggataatctc cgaacgctgc ccgagggcac tccccgggac tgcgagcgcg
     4021 aaatggccga cgccctgcac caagccagcg accgcccgat actgatcgca ccaggctgca
     4081 cgtacgatcc cgagcgagtc ccgcgggcca acctgctggc ggtctgccgc gcggtgagag
     4141 cgtcagcagg ggctgccagc cagatgaccc acacgcaagg gcgttgtggg tgataaagtg
     4201 ataaagtgat agagtgataa agtgttccgc agaccatcct ttatcacctt atcaccttat
     4261 cactttatca ccctttcata atagctcgat ccctcccaaa caatggcacg cctcccgctg
     4321 gccagcttac aatcccaaac atgagtgacg ctccgaaact ggtcgtcggc accgccggcc
     4381 atatcgatca cggcaagagc cgcttagttt gggttctcac cggaaccgat cccgaccgcc
     4441 tgcccgagga aaaggtccgc ggcatgacca ttgacctggg ctttgcccac gggcagttcg
     4501 acggctgcga tatttccttt gtggacgtgc ccgggcatga gcgctttatc cgaaacatgg
     4561 tggccggggc aacgggtatc gatgtcgccg tgctcgtagt cgccgcggat gactcggtca
     4621 tgccgcagac gcgcgagcac gcggaagtcc tcagtttgct gggcgtcgag cgctgcatcg
     4681 tggcgctgag caaaatggac ctggtggacg acgaatgggc cgacgcggtc gaggaggaag
     4741 tgcgcgggct gctgaccggc ttcggcatcc agccgctatg ttgcgtgcgt acctccgctg
     4801 aagatggccg cggggtggag gagttgcgcg gggtgctcgc gcggttggcg cgcgaacaga
     4861 gccggcaggc cgagccatac cgctggttct gcttgccgat cgaccgctcg ttcgtcattc
     4921 ccgggcgcgg cacagtcgtg accggctcgg tcgcacatgg aacagtgagc cgcgaggacg
     4981 agttggagct gtggccgacg gccgagcgcg tccgggtgcg tgatctccag tcgcaccatg
     5041 atgcccgcga ttcggcggcc gggcggcggc gactggcctt gaacctggcg ggagtgtcgc
     5101 gcgaggacgt ggcgcgcggt tacacactgg ccacgcccgg atatctggag ccggcgcggt
     5161 ttttggacgt gcggctggct acgctgcgca tgccgggcaa gacgcgcaag gacgtcttgc
     5221 ggctgcgctt gcacattgcg acgtccgagg tgctggcgga gttgcggctg gcggacaagc
     5281 ccgagagcga aaccgtatcg ggcgtcttcg ctcaagtgaa gaccgccgag cccatcgtcg
     5341 ccacctgggg ccagcgcttc atcttgcgtg acgaaagcgg catgcgcacg ctgggcggcg
     5401 gccgcgttct gcgccccgtc gggcggctct ggactgcgcg gcgcccgata caacgccagg
     5461 gcttgcagac cttgtttgaa ggccagcccg ccgcgcgctt ggaagaggtc atccgcgcgg
     5521 ccg
//
LOCUS       DAOSYS010000239         5517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12390_length_5517_cov_4.515745, whole genome shotgun sequence.
ACCESSION   DAOSYS010000239 DAOSYS010000000
VERSION     DAOSYS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12390_length_5517_cov_4.515745"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PLU99_12875"
     CDS             <1..595
                     /locus_tag="PLU99_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17985.1"
                     /translation="ETKSKTTTSMAGEERTQETTTLTVYDGEYVWMLSEIGEQKRVLK
                     TRPTTEPTLATNKAFFDSLKESYELTLLPDETLDGHATWVIQATPRQPSPQEGMPSVL
                     VLNFDKDTAANLKTVGKDSGGKEVMTISTKDVRLNPPLAADHFAFKVPEGVEVIDMTD
                     NQPAQPQTQPGAAKSEEPKPAEMPKKEPVKPAVPSNP"
     gene            607..1440
                     /locus_tag="PLU99_12880"
     CDS             607..1440
                     /locus_tag="PLU99_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO17986.1"
                     /translation="MRIRLAHSPDPDDAFMFYGLAQGAIDAGPYEFEHVLADIQTLNE
                     RAQAGDYEVTAISVHAYPYVCDKYALTACGSSMGDNYGPLVVSRAPMSVAQLGGQSIA
                     VPGRMTTAFLVLQLLLGKDGFTPQVVMFDQIPSEVLGGRVAAGLLIHEGQLTYAQQRL
                     HAVIDLGVWWHERTGLPLPLGGNAIRRDLGQQRCREITDIIRRSIRYGLEHRAEAVRY
                     ALQFGRGLDTELADRFVGMYVNEWTLDYGPRGREAVRRLLADGAAAGLVPPVSRLDFI
                     D"
     gene            1875..2987
                     /locus_tag="PLU99_12885"
     CDS             1875..2987
                     /locus_tag="PLU99_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOO17987.1"
                     /translation="MSSSLGTGVYTVELEDLTWNPTAQSALWVRSTPLQITDEASGQV
                     LATFTGVTLRLIRGSRIDLIANCRAGAADVTVELQTGHLGFTTISAATAQGMASATLN
                     VADLDGDGATVTGVGPPGTGIHRSFINQAYGSGTLFSTLLGSVEVGSGASGSAVQTDP
                     ASGYRAVGAAVSSMSVLVAFTVTANDQAGINSIFAIQPLPEDVGFDSDGDGRPDFIDA
                     CPADPTKIGPGACGCGVPDTDTDVDGVADCIDNCPAIPNPDQADSDGDGIGDACDDDE
                     LEIWEGPVELQPPGGVANHGHWDTGAAEAQTLDGDSGSVKGEGGEGYGTGTTPDELPP
                     SDADLIAFVPACGPMTFASIWLTILGLDALRMRRRW"
     gene            3058..4032
                     /gene="rarD"
                     /locus_tag="PLU99_12890"
     CDS             3058..4032
                     /gene="rarD"
                     /locus_tag="PLU99_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOO17988.1"
                     /translation="MYRDAPWAAPASITPSEDQPATALRARAAARAGVLYGVAAYGLW
                     GVFPVYFKAVQGVSPLEVLAHRVIWSVLFLALLLVVGRNNGTVAAALRSRRALLILCV
                     TALLIASNWLVFIWAVANGYLLQSSLGYFINPLLSVLLGLVFLRERLRRWQTVSVVLA
                     GVGVVYLTIASGEAPGIALALAGTFAIYGLLRKIAPVPALPGLAVETAILAPIALAYL
                     VWEMAQGRAAFGSAGLKMSLLLMAAGVITATPLLWFTQAARRLRLATLGFLQYLAPTG
                     HFLLAVLAYGEQFTQVHAIAFGCIWAALIIYSVDTMRAAQVLPVTKPE"
     gene            complement(4060..4884)
                     /locus_tag="PLU99_12895"
     CDS             complement(4060..4884)
                     /locus_tag="PLU99_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOO17989.1"
                     /translation="MQSPAKRCGYLAALLLGCAPWLLGCLPAQQRETEFQLQKAQTER
                     DAAEIALRDELARSAALSEQLTAMQQRAASAQAEAAAVREQLQSVQEQRDQALALIEQ
                     RASTPLARPDAPPSILPPDCDAALRNFVAKFQHRVWYDRSRGALSFANDQLFESGSDV
                     VRADAQASLHELAAVTNRALPRDFELIVVGHTDDAPITKPETLAQHPSNWHLSVHRAI
                     AVKDVLVKAGVSADRFGVMGYGAERPLGADRARNRRVEIFFTRKGDVQSFAPVKPE"
     gene            complement(5030..>5517)
                     /locus_tag="PLU99_12900"
     CDS             complement(5030..>5517)
                     /locus_tag="PLU99_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO17990.1"
                     /translation="IDDQVSLEGGGSSGSGLLDLTRESDDTSLGAELLDEIYPGEDES
                     ASKPQAAEAAPEPAAPAAEPQAVALAAAAPAEVLEVVAPLRTQVIDQYEGLFAGFLVS
                     AILLLGLAGSVVGGVLQGFLPDYATLLAHYFYPFLAAACGLVVVSAVVGMLLGRVFMR
                     R"
BASE COUNT         1018 a         1679 c         1843 g          977 t
ORIGIN      
        1 cgaaaccaag agcaagacca cgacctccat ggcaggggag gaacggacgc aagagacaac
       61 gaccctcaca gtctacgacg gcgagtacgt ctggatgctg tccgaaatcg gcgaacagaa
      121 gagagtcctg aagacacgcc cgaccaccga gcccacgttg gcgaccaaca aggcgttctt
      181 cgatagtcta aaggaaagct acgagctgac attgctgccg gacgagactc tcgacggcca
      241 tgccacctgg gtcatccagg ccacgccgcg tcagccatcg ccgcaagaag gcatgccaag
      301 tgtgctggtc ctgaatttcg ataaggacac ggccgccaac ttgaagacgg tgggcaagga
      361 ctccggcggc aaagaagtca tgactatcag cacgaaggac gtaaggttaa atccgccgct
      421 cgccgccgac cacttcgcct tcaaggtccc cgaaggcgtc gaggtcatag acatgacgga
      481 caaccagcca gcgcagccgc agactcagcc gggcgctgca aaatccgaag agccgaagcc
      541 ggcggagatg ccgaagaaag agccggtcaa gccggcggtc ccgagcaacc cgtaacggcc
      601 tggaccatgc gcattcggtt ggcgcacagc ccggaccccg atgacgcctt catgttttac
      661 ggattggcgc agggcgccat tgacgccggg ccgtacgaat tcgagcacgt tctggccgac
      721 attcagacgc tgaacgagcg tgcccaggcc ggtgattacg aagtcaccgc catcagcgtt
      781 catgcgtatc cctacgtctg cgacaagtac gcgctgacgg cctgcggctc cagcatgggg
      841 gacaattatg ggccgctggt tgtcagccgc gcgcctatgt ctgtcgccca gcttggcggc
      901 cagagcatcg ccgttccggg gcgcatgacc acggcttttt tggtgttgca gcttctgctg
      961 ggcaaggacg gtttcacgcc gcaggtggtc atgtttgacc agattccgtc cgaagtgctg
     1021 ggtggccgcg tggcggcggg cctgctgatc catgaagggc agctcaccta cgctcagcag
     1081 cggctgcacg cggtaatcga tcttggcgtg tggtggcacg aacgcacggg actgccgttg
     1141 ccgctgggcg ggaacgcgat ccgccgcgac ctcggtcagc agcgctgccg cgaaatcacg
     1201 gacattattc ggcgcagcat tcgctacggc ttggaacacc gggcggaggc ggtgcgttac
     1261 gcgctgcagt tcggccgtgg gctggacacc gagttggccg atcgcttcgt gggcatgtat
     1321 gtcaatgagt ggacgctgga ctatgggccg cgcgggcgtg aagcagtgcg gcgtctgctg
     1381 gcagatgggg ccgcagccgg cctggtgcca ccagtctccc ggctggactt catagattga
     1441 ctaaacttca tagatagagt cgtacggggg acgggtggac cggcgggtgc catgcccaag
     1501 ctgtaagcgc gggcatgccc ccgtcgcaaa ccgctgccca aacgggccat ggcgggagta
     1561 gaaccgagac cgaccgggca agcccgataa cgcgagaagg cgtctcggca agtcgcacaa
     1621 accccctcca cacgccaata aaagctgacg ctcggaactc acgcgcccgg catccctttt
     1681 tttcttgcac cggcgccgca ccattggcac aatcgactaa gttggcttcc catgccggga
     1741 agcgttagga ggagaaagac gtgacaacgg tcagtaagct ttgcgcaatc gcgacatgta
     1801 tattcatggt cgcggcctgg cctggccagt ctgccgcggc cgtttctttt tctggcaccc
     1861 cgctgcaaat cgaagtgagc agctcgctgg gcacgggcgt ctacacggtc gaactggagg
     1921 acttgacgtg gaacccgacc gcgcaaagcg cgctttgggt tcgcagcacg ccactccaga
     1981 tcacagacga agcctccggg caagtgctgg cgacattcac cggcgtgacg ctacggctta
     2041 tacgcggcag ccgaatcgac ttaattgcaa actgccgagc cggcgcggcc gacgttacgg
     2101 tggagttgca aacggggcat ctgggtttca cgacgatttc ggccgcgacg gcgcagggga
     2161 tggcctcggc cacgctgaac gttgcggatt tggacggtga cggggccacg gtgaccggcg
     2221 tcggcccacc cgggaccggc attcatcggt cgttcatcaa ccaagcctac ggctcgggca
     2281 ctcttttctc gacgctgctc ggatcggttg aggtcggatc gggggcttcg ggctcggccg
     2341 ttcaaactga tccggcctcc gggtaccgcg cggtgggcgc ggcagtcagc agcatgagcg
     2401 tgctggtggc gttcaccgtg acggccaacg accaggccgg cataaactcg atctttgcca
     2461 ttcaaccgct gccggaggat gtcggcttcg atagcgacgg tgacggccgc ccggatttca
     2521 tcgacgcgtg ccctgctgac ccgaccaaga tcgggcctgg tgcgtgcggc tgcggcgtgc
     2581 cggacaccga tacagacgtg gacggcgtag cggactgcat cgataactgc ccggcgattc
     2641 cgaatcctga ccaggcggat tcggacggcg acggcattgg cgacgcctgc gacgatgacg
     2701 agctggaaat atgggaaggc ccggtggaac tgcagcctcc gggcggagtt gccaaccacg
     2761 gccattggga tactggcgcc gctgaagcgc aaacgctgga cggcgactca gggagcgtga
     2821 agggtgaggg cggcgaaggc tacggaacag gaacaacgcc cgatgaatta cctccgtcgg
     2881 acgcggacct gatagctttc gttcccgctt gcggaccgat gacgtttgcc tcgatctggc
     2941 tgacgattct cgggctggac gccttgcgga tgcggcgcag gtggtaaggg atcggccata
     3001 acacggcaaa aaaacgcagc gagcaaggga gaaactggcg ggttacaata gccgcccatg
     3061 taccgtgacg cgccttgggc ggcgcccgcc agcattacgc caagcgagga tcagcctgct
     3121 acagcgttgc gcgctcgtgc cgccgcgcga gccggagtgc tctacggcgt ggcggcctat
     3181 ggcttgtggg gggtgtttcc ggtttatttc aaagcggtcc aaggcgtctc gcccctggaa
     3241 gtgctggccc atcgggtgat ctggtcagta ctgttcttgg cgctgctgct cgttgtgggg
     3301 cgcaacaatg ggacagttgc ggcagcgctc cgttcgcggc gagccctgct gatcttgtgc
     3361 gtgacggcgc tgctcatcgc gtcgaactgg ctggtgttca tctgggcggt ggccaacggg
     3421 tatctcctgc aatcgagcct ggggtacttc atcaacccgc ttctaagcgt gctgctgggg
     3481 ctggtcttct tgcgtgagcg gctgcggcgc tggcagacgg tgagcgtcgt gctggccggc
     3541 gtaggggttg tgtatctcac gatcgcgtcg ggcgaggcgc cggggattgc gctggcgctg
     3601 gcgggaacgt ttgccatcta cgggctattg cggaagatcg caccggtgcc ggccttgccc
     3661 gggctggcag ttgagacggc catcctcgcg cccatcgcgc tggcttacct ggtgtgggag
     3721 atggcgcagg ggcgtgccgc gttcggcagc gcggggctga agatgagtct gttgctgatg
     3781 gcggcgggcg tgatcaccgc gacccccctt ctgtggttca cgcaggcggc ccggcggctc
     3841 cggctggcca cgctcgggtt cctgcaatac ctggcaccga cgggccattt cctgctggcg
     3901 gtcctggcgt acggggagca attcacgcaa gtccacgcca ttgcgttcgg ctgtatctgg
     3961 gcggcgctga tcatctattc agtcgatacg atgcgcgcgg cccaagtgct gccggtcacc
     4021 aagccggagt aggcagattg cgcttcccct ggcttttctt tactccggct tgacgggtgc
     4081 gaacgactgc acgtcgccct tgcgggtgaa gaaaatctcg acgcgccggt tacgggcgcg
     4141 gtcggcgcct agcgggcgtt cggcgccgta gcccatgacg ccgaaacggt cggccgaaac
     4201 gccggccttc accagaacgt ctttcactgc gattgcgcgg tgtaccgaaa gatgccaatt
     4261 gctcgggtgt tgcgccagcg tctcgggctt ggtgatgggc gcatcatcgg tatggccgac
     4321 gacgatcaat tcgaagtcgc gcggcagagc gcggtttgtg accgccgcca gttcgtggag
     4381 cgaagcttgt gcgtccgcgc ggactacgtc gctgcccgac tcgaagagtt gatcgttggc
     4441 aaaactcaga gcgccgcggc tgcggtcata ccagacgcgg tgctggaact tggcgacaaa
     4501 gttccgcaag gcagcgtcac aatcgggcgg gagaatcgag ggcggcgcat ccgggcgagc
     4561 cagcggcgtg ctggcgcgct gttcgattaa ggctaaggct tggtcgcgct gctcttgcac
     4621 cgactgcagt tgctcgcgaa cggcggccgc ttcggcttgg gcggaggcgg cgcgctgctg
     4681 catagcggtg agctgctccg acagtgccgc gctgcgggcg agctcatcgc gcagggcgat
     4741 ttcggccgcg tcgcgctccg tttgagcttt ctggagctga aactcagtct cgcgctgctg
     4801 agcgggaagg cagccgagca gccaaggtgc gcagcccagc aacagagctg ccagatatcc
     4861 gcagcgtttc gcaggagatt gcacgtggtc tcctcctcct ggcaaattga gggatctagg
     4921 gatcgaggga ttgagagatc aagaacgcac cgaaagcgat cttctcttga tccctagatc
     4981 ccttgattcc tcgatccctg ttctcaaaca ggggcgccga ggcccctccc taccggcgca
     5041 tgaaaacgcg gccaagcagc atgccgacca cggccgagac gactaccagg ccgcaagccg
     5101 ccgccaggaa ggggtagaag tagtgcgcca acagcgtggc gtaatcgggc aggaagccct
     5161 gcaatacgcc gccgaccacc gaccccgcca gtcccagcag caatatcgcc gaaacgagaa
     5221 atccggcaaa tagaccttcg tattgatcaa ttacctgcgt gcgaagcgga gcgactacct
     5281 cgagcacctc ggccggggca gccgcggcca aagcgaccgc ctgcggctca gccgcgggcg
     5341 ccgccggctc gggagcggcc tcggcggctt gcggcttgga ggcgctttcg tcttcgccgg
     5401 ggtagatttc gtccagcaac tcggcgccga gcgaggtgtc gtcggactcg cgcgtcagat
     5461 ccagcagacc cgagccgctg ctgccgccgc cttcaagcga aacctggtca tcaatcg
//
LOCUS       DAOSYS010000240         5464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12551_length_5464_cov_5.207062, whole genome shotgun sequence.
ACCESSION   DAOSYS010000240 DAOSYS010000000
VERSION     DAOSYS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12551_length_5464_cov_5.207062"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /gene="nadA"
                     /locus_tag="PLU99_12905"
     CDS             complement(<1..1032)
                     /gene="nadA"
                     /locus_tag="PLU99_12905"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOO17991.1"
                     /translation="MIEPTLSQQELPPNVTRLDREELCAGIAAAKQRLGRRLVILGHH
                     YQQDEVIQFADFVGDSLKLSQLAAAQTDAEYIVFCGVHFMAESADILTSERQAVILPD
                     LAAGCSMADMAHVDQAEAAWEVLAPWAGAGITPITYVNSSAAVKAFVGRHDGACCTSS
                     NAKMVLEWALHGAAKSGGGHKVLFLPDQHLGRNTGYALGWPLESMVVYDPELPEGGLT
                     REQVRAARFILWKGHCSVHQLFNTQQIAAFRAAPEPWQIIVHPECTWEVCQQADLVGS
                     TEYIVRTVREAPAGSYWAVGTEVHLVSRLANTMPDKQIRLLAGVQCLCSTMFRIDLPH
                     LYWVLDRLLH"
     gene            complement(1025..1480)
                     /locus_tag="PLU99_12910"
     CDS             complement(1025..1480)
                     /locus_tag="PLU99_12910"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOO17992.1"
                     /translation="MSSKTTARLSRWQHWYRGALPAYWVYLVCMTHFPRLRLKTHVTA
                     ADKVAHVVCFALLAFLFWRFAETFRKPAPPWLVWLALAWLGLYAALDEWTQSFVGRGT
                     DVQDWLADMLGIMAVLAVLEWRRRAKAARAAAIPGRRELLPAQQESTND"
     gene            complement(1477..2421)
                     /locus_tag="PLU99_12915"
     CDS             complement(1477..2421)
                     /locus_tag="PLU99_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOO17993.1"
                     /translation="MRSIAIINQKGGAGKTTTAVNLSDALAREGRRVLLVDLDPQSHA
                     SLHLGVQLGEDEAGVYDVLVGDAAAAEAMRSVNERLALLPAGIDLVGAELELRQRPNW
                     QRVLRDVLAPYREAFDFCIIDCPPSLGQLTLAALVAVEEVIIPLQPHFLALQGLGRLL
                     ETVTLVRQDLNPGLRVSGVALCMFEKGTRLAQEVLEDIQRFLSAAEPGTAWYGARVFD
                     TRVRRNIKLAECPSYGQTIFAYASGSNGAEDYHALALEVLGLANGARHEISGDGQAVA
                     ESIAEGATAAGARGRSEEPDAVVGSTSIVAGPAECDGI"
     gene            complement(2522..3118)
                     /locus_tag="PLU99_12920"
     CDS             complement(2522..3118)
                     /locus_tag="PLU99_12920"
                     /inference="COORDINATES: protein motif:HMM:NF023496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HOO17994.1"
                     /translation="MDELDIGPGLVGQRVYNPARPEWGAGTVLRVETSRGDAGLQHRV
                     SVQFLAGHKMVHIPPAKLTRELEGQTREAGWLDTVAGSTPDERLVRLPPEVTGFLGTA
                     SQRLALVAALYEYTDEPGSLINWARRQTRIADPLSHWSRDELVQAFAKFCGERDAELR
                     VTAARVKQAQGAEGLQAALDALAPHIRQAVKAALQRPI"
     gene            3253..3957
                     /locus_tag="PLU99_12925"
     CDS             3253..3957
                     /locus_tag="PLU99_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO17995.1"
                     /translation="MALRQRRREPEDMDDPALQSEQYAHALNSLSRLNFAAGTAQVFW
                     RHLRPIMRTRPRGTWRLLDVATGGGDIPLRLWRLARNERFDLQIAGCDISERAVHQAQ
                     ARADAAGANVQYFRQDVVREPISGEYDFVISSLFIHHLSDGEAICAFRNLAPATRRLL
                     LINDLRRCLWGLLVAFVATRVLTTSRVARVDGLRSVRAAYTMAEIRALATAAELTQAK
                     VLRHWPARWLLEWSRP"
     gene            3954..5210
                     /locus_tag="PLU99_12930"
     CDS             3954..5210
                     /locus_tag="PLU99_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO17996.1"
                     /translation="MSPQAPVLHTPSLDSPDLPAIDAAAAALQLWDVIVIGAGPAGAM
                     AALRLARQGARVLLVEKQRFPRPKVCGCCLNAAALELLAECGLADLTSRAGAQPLSAI
                     ELGVAGKTARLGLPTGAALSRERLDALLVGEAIAAGASFLPATAAGVGPVENDWRRVA
                     FSSAGQGFDTAAKVILVADGLGGRALRECPESEAFTAAQARVGGNAVLAGPIRGYGPG
                     VIYMACARGGYVGLVVLESNRVDLAAAVDVNYIRRHGGLARSAARILAEAGFPPVPGL
                     EAATWHGTPRLTARRRPLAFERVFLIGDAAGYVEPLTGEGMAWAMATGTAVVPFALAA
                     MAGSGANAWGSPPAATARERSLADGWASCYRRLLAARQRRCHVITRLARHPAIAWLAV
                     VALARFPILARPTIAGINRPLLRPVR"
     gene            5391..5463
                     /locus_tag="PLU99_12935"
     tRNA            5391..5463
                     /locus_tag="PLU99_12935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5425..5427,aa:Glu,seq:ttc)
BASE COUNT          876 a         1904 c         1728 g          956 t
ORIGIN      
        1 gtgcagcaag cggtcgagca cccagtacaa atgcggcagg tcgattcgaa acatcgtgga
       61 acacaggcat tgaacgccgg ccagcaggcg aatctgcttg tccggcatcg tgttagccag
      121 ccggctcacc aggtgtactt cggtgcccac tgcccaatag gaacccgccg gggcctcgcg
      181 aaccgtcctg acgatgtact ccgtgctgcc cactaggtcg gcctgctggc agacttccca
      241 cgtacactcc ggatgcacga tgatctgcca cggctcgggc gccgcccgaa acgcggcaat
      301 ctgctgcgta ttgaaaagct ggtgtacgga gcagtgcccc ttccacagga tgaaccgcgc
      361 cgcacgcacc tgctcgcgcg tcagaccgcc ctcgggcagc tccggatcgt agacgaccat
      421 gctctccagc ggccagccga gtgcgtagcc ggtgttacga cccaggtgct gatccggcag
      481 gaataggacc ttgtgcccgc cgcctgactt ggccgccccg tgcagcgccc actccagcac
      541 cattttcgca ttcgagctcg tgcagcaggc cccgtcgtgc cgccccacaa acgccttgac
      601 ggccgcggag gagttgacgt aggtgatggg tgttatgccc gcgcccgccc aaggcgctag
      661 cacttcccat gccgcctccg cctgatccac gtgggccatg tcggccatgc tgcagccggc
      721 cgccaggtcc ggcaggatca ccgcctgccg ctcgctggtg agaatatccg ccgactcggc
      781 catgaagtgt acgccgcaaa agacgatgta ctcggcgtca gtctgcgccg cggcaagctg
      841 cgagagcttc aagctgtcgc cgacgaaatc ggcgaactgg ataacctcgt cctgctggta
      901 atgatgtcca aggatgacca gccgccgccc caggcgttgc ttggcggccg cgatgccggc
      961 gcacagttcc tcgcgatcca ggcgcgtgac attcggcggc agttcctgct gacttaaggt
     1021 gggctcaatc attagtggac tcctgctgcg ctggaagcag ctcgcgccgg ccggggattg
     1081 cggcagcgcg ggcggccttt gcccggcggc gccactccag gaccgcgaga accgccatta
     1141 ttcccagcat atccgccagc caatcctgta cgtccgtccc gcgcccgacg aaactctgag
     1201 tccactcgtc cagcgccgcg tacaggccga gccatgccag cgccagccag accagccagg
     1261 gcggcgcggg tttgcgaaac gtctccgcaa agcgccagaa caggaaggcc agcaaggcga
     1321 aacatactac gtgagccacc ttgtcggccg cggtcacgtg cgttttgagc cgcagtctgg
     1381 ggaagtgcgt catgcacacg aggtagaccc agtaggccgg cagcgcgccg cgataccaat
     1441 gctgccagcg cgagagtcgc gctgtggtct tcgagctcat attccatcac actcagcagg
     1501 acccgccacg attgacgtgc tccccaccac cgcgtcaggc tcttccgaac gccccctagc
     1561 cccggcggcc gtcgcccctt ccgcaatgct ctcagcaact gcttgcccgt cgccgctgat
     1621 ttcgtggcgc gccccattcg ccagccccag cacctccagg gccagcgcgt ggtaatcctc
     1681 ggcgccgttg ctgccggacg cataggcgaa aatcgtctgc ccataactcg ggcactcggc
     1741 cagctttatg tttcgccgca cgcgcgtatc gaagacccgc gcaccgtacc aggccgtgcc
     1801 aggttccgcc gcgctcagga agcgctgtat gtcttcgagc acctcctgcg ccaggcgggt
     1861 gcccttctcg aacatgcaca gcgccacgcc ggacacccgc aaccccggat tcaagtcctg
     1921 ccgcaccaac gtgaccgtct ccagcaagcg ccccagcccc tgcaaggcca gaaagtgcgg
     1981 ctgcagcgga atgatcactt cctccaccgc gaccagcgcc gccagcgtaa gctgtcccag
     2041 cgaaggcgga cagtcgatga tgcagaaatc aaacgcctcg cgatacggcg ccaaaacatc
     2101 ccgcagcacg cgctgccagt tcggcctctg acgcagctcc agctccgctc cgaccagatc
     2161 gatcccggcc ggcagcaaag ccaatcgctc gttcacgctc cgcatcgctt ccgcggccgc
     2221 agcgtcgccg acgaggacgt cgtaaacgcc cgcctcatcc tcgccaagct gcacgcccag
     2281 gtgcaggctg gcgtgcgatt gcggatccaa atcaaccagc agcacccgcc gcccctcgcg
     2341 cgctagcgca tccgagaggt tcaccgcggt ggtcgtctta cccgcgccgc ccttctgatt
     2401 gatgatcgca atcgaccgca tgttctcagt ataagcgcgg gctggcggcg gcgggagcaa
     2461 aaaagccggc ggcaatctgc cccaccgtga atcgccagat gcccaaatcg tcaaatccga
     2521 atcaaatcgg gcgctgcaac gccgctttca ccgcttgacg tatgtgcggc gccagcgcat
     2581 ccaacgcggc ctggagacct tccgcgcctt gggcctgctt cacccgcgct gccgtgacgc
     2641 gcagttccgc atcccgctct ccgcagaact tagcgaacgc ctgaacgagc tcgtcgcgcg
     2701 accagtgcga cagcggatca gcaattcgcg tctgccgccg agcccagttt atgagcgagc
     2761 ccggctcatc cgtgtattcg tataacgcgg ccaccaacgc cagccgctgc gacgccgtgc
     2821 ccaggaaccc cgtgacctcc ggcggcaagc gcaccaggcg ctcgtcgggc gtgctgccgg
     2881 caacggtatc caaccagccc gcttcgcgcg tctggccctc caactcgcgc gtcagcttgg
     2941 ccggcgggat gtgcaccatc ttgtgcccgg cgagaaactg cactgaaacc cggtgttgca
     3001 gcccggcgtc gccgcggctg gtttcgacgc gcagaacggt acccgctccc cattccggac
     3061 gcgcggggtt gtacacccgt tgtccgacca gtcccggtcc gatgtccaac tcgtccatgc
     3121 cagtggtttc cattcggcga cggccgcccc gtctcgacgc tattatcctt cgcctgtccg
     3181 gctgcgtcac cgggctcgac tcagatacct tattttgtgc tcaggtcgcc cattttcaac
     3241 atgcagcctg cgatggcatt gaggcagcgc cgccgcgagc ccgaggacat ggacgacccc
     3301 gcgctccagt cagaacagta cgcccacgcc ctgaacagct tgtcgcgcct caatttcgcc
     3361 gccggcaccg cgcaggtttt ctggcgccac ctgcgcccca tcatgcgcac gcgcccacgc
     3421 ggaacgtggc gtttgctgga cgtggcaacg ggtggcggcg acatcccgct gcggctgtgg
     3481 cgcctggccc ggaacgagcg cttcgatctt caaatcgccg gatgtgacat cagcgagcgg
     3541 gcggtccacc aggcccaagc gcgggctgac gccgcaggcg cgaacgttca gtacttcagg
     3601 caggacgtgg tgcgcgagcc gatttccggc gagtacgact tcgtcatctc ctcgcttttc
     3661 atccaccatt tgtcagacgg tgaggcgatt tgtgcatttc gaaatcttgc gccggctacg
     3721 cggcgccttt tgctcattaa cgatctacgg cgctgcctgt gggggttgct cgtcgctttc
     3781 gtagcaacgc gagtattgac cacctcgcgc gtggcgcgcg tggacggcct gcggtccgtt
     3841 cgtgcggcgt atacgatggc cgagattcgg gcgctcgcca cggccgccga gttgacgcag
     3901 gccaaagtcc ttcggcattg gcccgcgcgc tggctgcttg agtggagccg gccatgagcc
     3961 cacaggcccc cgtgctgcac acgccgagtc tggattcgcc tgatttgcct gcgatagatg
     4021 ccgcggccgc tgcgctgcaa ctctgggacg tcatcgtgat aggggcgggc ccggccggcg
     4081 cgatggctgc cttgcgcctg gcacgacagg gggcgcgcgt gttgctcgtg gagaagcagc
     4141 gctttccccg acccaaagtc tgtggctgct gcctgaacgc ggccgccctt gagctgctcg
     4201 ctgagtgcgg cctggcggat ttgaccagtc gcgcgggcgc gcagcctctg tcggccattg
     4261 aactgggcgt tgccggaaag accgcgcggc ttggtttgcc aacgggggcg gcgctgtcgc
     4321 gcgagaggct tgatgccctt ctggtcggcg aggccattgc cgccggcgct tcgtttttgc
     4381 ccgcgacggc ggcgggtgtg gggccggtcg agaatgactg gcggcgcgtc gctttttcaa
     4441 gcgcgggtca gggttttgac accgccgcaa aggtgatcct ggtcgccgac ggccttgggg
     4501 ggcgggcgct gcgcgagtgt ccggaatcgg aggcattcac ggccgcgcag gcgcgcgtcg
     4561 gcgggaatgc ggtcctggcc ggcccgatcc gcggctacgg gccgggcgtc atctacatgg
     4621 cctgcgcacg cggcggttac gtcgggctgg tcgtgctcga aagcaaccgc gtcgatttgg
     4681 cggccgcggt tgacgtcaat tacatccggc gtcatggcgg gctggcgcgt tcggctgccc
     4741 ggattctggc agaggccggc tttccgccgg ttcccggtct tgaggccgca acgtggcatg
     4801 gaacgccgcg cctcaccgcc cgccgacggc cgctggcgtt tgagcgcgtt tttttgatcg
     4861 gagatgcggc cggttacgtc gagccgctga cgggcgaggg catggcgtgg gcgatggcca
     4921 cgggcacggc tgtcgtgccg tttgcactgg ctgcgatggc cggctcaggt gcaaacgcct
     4981 ggggctcgcc gccggcggcg acggctaggg agcggtcact ggcggacggc tgggccagtt
     5041 gctatcggcg actactggcg gcccgtcaac gccgctgcca cgtcatcacg cgcctagccc
     5101 gtcatccggc gatagcatgg ctagccgtcg tagccctggc ccgttttccg atactggcgc
     5161 gtccgacgat cgcgggcatc aatcgcccgc tgctgcgacc ggtgcgctga gcggagctgg
     5221 ctcggatcac gtgccctcgc gcttcaggct cggattggag aaccggttgc cctcgctgcg
     5281 ggctcggagc ggagaaccgg ttgccctcgt cgcgcttcgg gctccggtta accgcccgcc
     5341 ttgaccatgc cgcgaggcgg ccgtagtatg gcccttttgc aacgcggcct gcccccatcg
     5401 tctagaggcc taggacacgg gcttttcaag cccgcgacac gggttcgaat cccgttgggg
     5461 gcgt
//
LOCUS       DAOSYS010000241         5446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12594_length_5446_cov_5.016694, whole genome shotgun sequence.
ACCESSION   DAOSYS010000241 DAOSYS010000000
VERSION     DAOSYS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12594_length_5446_cov_5.016694"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..716
                     /locus_tag="PLU99_12940"
     CDS             366..716
                     /locus_tag="PLU99_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO17997.1"
                     /translation="METYPVARSYSAPRNFPLPLNTFERPGRALFSQSDWAALGRKLN
                     LTKRQLEVAELVCEELTYSDIATRMGISVNTVRMHMRGLFFTLGVRDRVGVVLRLVLT
                     QRSLLNDEAKAVIE"
     gene            880..1920
                     /locus_tag="PLU99_12945"
     CDS             880..1920
                     /locus_tag="PLU99_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOO17998.1"
                     /translation="MPRGDQVLRQWNLLKMLQTRGQGLSLRELAEENGVSERTIQRDF
                     ETLQEMGFPVDHDQDQCGKRFWRLPHDFFKTAPLVLSLTEAISLHLAEHLFEPLAGTL
                     FADGLATIRDKIRSQLPAKALEYFRGLDEVVYVRRTGLTDYSAHADTIRTLTEAAQQN
                     RAVDIAYDSLWRGERYRTLVDPFGLVLYEGDLFLAGFSHKAGAIRIFKVTRITTAAPT
                     QQSFERPADFRLEEHFRNTFGIVRSSGPPVEIVVRFTGPAAALVEERLWHESQQVERL
                     ASEPTLFEELDDGRPTLQATFKLADLVEFKRWIKGFGADAEVIRPESLRQELQAELRD
                     TLSRYESVQPPV"
     assembly_gap    2269..2368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2369..4698)
                     /locus_tag="PLU99_12950"
     CDS             complement(<2369..4698)
                     /locus_tag="PLU99_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOO17999.1"
                     /translation="MWSMGSRRVTLAVLAMAFASLPAIGAGPVTVTVLENTPDKIVVD
                     YQIGNHQQAVVTIDGQPYTVLSLPHEPVMLERGAPELPKVCRSIIVGPDAAMEASVVA
                     SEYYDITDIKVAPSKGNISRQIDPATVPYTFGPEYSANAFYPGQLVGLQAPYIMRDFR
                     GQVLELFPFQYNPGTETLRVYTHVRVEVARRGVSTENVQTPRRDRQVCAAFHQLYKHH
                     FINYVPETRSDPLEEQGSMLVICYDQWLPNIQPFVDHKNDILIETTAVGVSTIGTTSS
                     QIAAYIQNVYNTTDLAFVLLVGDVAQIPSPGPGARDPIYSRVDGSDNYPDIMIGRFSA
                     ENAAQVDLQVLKSIQYEDEQYTTTDWFWKAVGIASSEGSGIGDDGQSDWVHMDEIRGW
                     LTDYGYTTVDQIYATTGATDAMVTAAVNEGRGLINYCGHGAMSYWVTTGFDISDVNAL
                     TNYNKLPVIISVACNNGEFQSGTCFAEAWLRATRDGQPTGAAAMYASTISMSWAPPME
                     GQDEFNLLFTSEAYFAFGTLCFAGSCSMMDEYGSSGVNEFECWTVFGDPSLRIVGVAY
                     VPPLRMALASELPEYIAPEVATTIRVSIEPGTETLVADSPTLHYSYGADFASVPMVNV
                     EGALWEGTLPPPACGTRPVYYFTAQGNLGTLVQLPTLEDGGTYTASVGNVSVLLDDDF
                     ENERGWTVFNDPSLTGGGWERVVPLAGGGATVPVADYDGSGRCYITDNRYSSDVDFGP
                     TILTSPVLDAGNGGDLILKFARWLTCDDELPPAMDYL"
     gene            complement(5247..>5446)
                     /locus_tag="PLU99_12955"
     CDS             complement(5247..>5446)
                     /locus_tag="PLU99_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18000.1"
                     /translation="RLASDGYDPVYGARPLKRLIQQQIENPLARRILAGEFGPGDEIS
                     VDARGERYVLEALTKDEPVPA"
BASE COUNT         1042 a         1775 c         1516 g         1013 t
ORIGIN      
        1 cggagccgaa ggttgcaggt tcgaatcctg cgcggcgcgt ttcttcccct gtgtacttac
       61 gaccgatcgc cccgaaccag aaaaaagctg gatttctgcc agcacgcgca tcccctgctg
      121 cttgcgttcc aaccgcgctt ccccaccaac atcttgagcg gccgaccccg gctgtcggac
      181 cctgacgcct gttggggccg acccgacctg tcggctggcg cggccagaat cagcttgacc
      241 ggtcgccgcc acgccggctg agcggcccac tgcaatgcga ttggagcagc cctttggcaa
      301 ttggggctgc ctccttgtga gggcgggcgg agcgcccccc gttgaagcga ctggagcaag
      361 aaagcatgga gacttacccc gtggcgcgtt cgtattcggc cccgcgcaac ttcccacttc
      421 ccctcaacac gttcgagcgg ccaggccggg cgctcttcag ccaatcggat tgggcggctt
      481 tggggcgaaa gctcaacttg accaagcgcc agctcgaggt cgcagagctg gtgtgcgagg
      541 agctgaccta ctcggatatc gcgacccgaa tgggcatttc ggtcaacacg gttcgtatgc
      601 atatgcgcgg cctgttcttc acgctcggcg ttcgagaccg ggtcggagtc gtcctccgcc
      661 tggttctgac gcagcgcagc ctgctgaatg acgaagcgaa ggcggtgata gagtgacact
      721 gtgatagagt gataaagtga tagagtgata aagtgattgc gcagacggtc cccttatcac
      781 cctatcacct tatcacctta tcaccgtacc gtttctttgg acgcctcaaa cgtcgtatca
      841 tgcgcttggc tcagtcggtt caactatgga gcgagcgtta tgccacgagg cgaccaggta
      901 ctgcgtcagt ggaatctgct caagatgctg caaacccgcg gacaggggct gtcgctgcgc
      961 gagctggccg aggaaaacgg cgttagcgag cggactattc aacgcgactt cgagactctg
     1021 caggaaatgg gttttccggt cgaccatgac caagaccaat gcggcaagcg cttctggcgc
     1081 ctgccgcacg atttcttcaa gaccgccccg ctcgtcttga gcctcaccga agcgatatcg
     1141 ctgcacctcg ccgagcacct attcgaaccg ctggcgggaa cgctgttcgc ggatggcctg
     1201 gccacaatcc gcgacaaaat ccgcagccag cttcccgcca aagccctgga gtacttccgc
     1261 ggcctggacg aagtggtcta cgtgcgccgc accggcctga ccgattactc cgcacacgcc
     1321 gacaccatcc gcacgctgac cgaagccgcg cagcagaacc gcgcggtcga catcgcctat
     1381 gactcactct ggcgcggcga gcggtacaga acgctggtcg atccgttcgg cctggtgctt
     1441 tacgagggcg acctgttcct ggccgggttc tcgcataaag ccggcgcgat ccgcatattc
     1501 aaggtcaccc gaataaccac ggccgcgccg acccagcagt cattcgagcg ccctgccgac
     1561 ttccgcctcg aggaacactt ccgtaacacg ttcggcatcg tgcgttcgag tgggccgccg
     1621 gtcgagatcg tcgtccggtt caccggaccg gccgcggccc tggtcgaaga acgcctctgg
     1681 cacgaaagtc agcaggtcga acgcctggcc tccgagccca cgctgttcga ggaactggac
     1741 gacggccgcc cgacactgca agcgacattc aagctggcgg atttggtcga gttcaaacgc
     1801 tggatcaagg gctttggcgc cgacgcagag gtaatccgcc cggaatcgct tcgccaggaa
     1861 ttgcaggccg aattgcgcga cacactgagc cgttatgaga gcgtccagcc gccggtataa
     1921 gtgaacccgc cgcgccacgc cggacgcacg aactcgccgc cttggtctgg atggtttgca
     1981 gccccggacc cggcatggcg taatgcccgt tgcacataaa gcaacgaccg gacccagcca
     2041 cttagccagg tccggccgct gcaaattcac tgcgtccgcc gtgacggcgt cagcccgtta
     2101 cgctcctcac tggaccaaca ggtcgacaaa tggatcgacg tcgaagacgt tgatttctcc
     2161 gtcgccgttg atatcggcat tattgatgtt gcaggccggg aaagccgcgg cgtactcggt
     2221 tgggctggtc aacgccagca cgaacgggtc aatgtcgaac gcgttgatnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnag ataatccatg gccggcggca gctcgtcgtc
     2401 gcacgtgagc cagcgcgcga atttcagaat cagatccccg ccattgccgg cgtccaacac
     2461 cggcgacgtc aggatcgtcg ggccgaagtc cacgtcgctg ctgtagcgat tgtccgtgat
     2521 gtagcaccgt ccggatccgt cgtaatccgc taccggcacg gttgcgccgc cgccggccaa
     2581 cggaactacg cgctcccatc cacctccggt caggctgggg tcgttgaaga ctgtccagcc
     2641 gcgttcgttc tcgaagtcgt catccagcag caccgacacg ttccccacac tggcggtata
     2701 cgtcccgccg tcctcaagcg tcggcaactg caccaacgtg cccaaattgc cctgcgccgt
     2761 gaaataatac accggtcgcg tgccgcaggc cggcggcggc agcgtgccct cccagagcgc
     2821 accctcgacg ttcaccatcg gcacgctggc gaaatccgcc ccgtaggaat agtgcagggt
     2881 cggcgaatcg gccaccagcg tctccgtccc aggctcaatg ctcacccgaa tggtcgtggc
     2941 cacttccggt gcgatgtact ccggcaattc actcgccagc gccatgcgca gcggcggaac
     3001 atacgccacg cccacgatcc gcagcgacgg atcgccgaac accgtccagc actcgaactc
     3061 atttaccccg ctgctgccat attcgtccat catcgagcac gaacccgcaa aacagagcgt
     3121 gccgaatgcg aaatacgcct ccgatgtgaa caacaggtta aattcatcct ggccttccat
     3181 cggcggcgcc caggacatgc tgatagtcga ggcgtacatc gccgccgcgc ccgtcggctg
     3241 gccgtcgcgc gtcgcccgca accaggcctc cgcgaagcag gtgccgctct ggaactcgcc
     3301 attgttgcag gccaccgaga tgatcaccgg taacttattg taattggtca gagcattgac
     3361 atcgctaatg tcgaaaccgg tcgtgaccca atatgacatg gcgccgtgcc cgcagtagtt
     3421 gattaacccg cgcccctcat tcaccgcggc cgtcaccatc gcgtctgtcg cgcccgtcgt
     3481 agcataaatc tggtcgacgg tcgtatagcc gtagtccgtc agccagccgc gaatctcgtc
     3541 catgtgcacc cagtccgatt gcccatcatc gccgatgccg gagccctccg aggaagcaat
     3601 tccgactgcc ttccagaacc agtcggtcgt tgtgtactgc tcgtcctcgt attggattga
     3661 cttcagcacc tgcagatcaa cctgggccgc gttttcggct gaaaaacgcc cgatcatgat
     3721 atccgggtaa ttgtccgagc catcaaccct ggagtagatc ggatcgcgcg cccccggccc
     3781 gggcgacggg atctgggcca catcgcctac caagagcaca aaagccaggt cggtcgtgtt
     3841 atacacgttt tgaatgtagg ccgcgatctg cgaggaggtg gtgccgatag tcgaaacgcc
     3901 cactgccgtc gtttcgatca ggatgtcgtt cttgtggtcg acgaagggct gaatgttggg
     3961 aagccactgg tcgtagcaga tgaccagcat gctgccctgc tcttccaggg gatcagaacg
     4021 ggtctcgggc acgtagttga tgaagtggtg cttatagagc tgatgaaaag ccgcgcaaac
     4081 ctgccggtcg cgccgcggcg tctgcacgtt ttccgtactg acgcccctgc gcgccacttc
     4141 gaccctcacg tgcgtgtaca cccgcaaggt ctccgtgcca gggttgtact ggaacgggaa
     4201 cagctccagc acttgcccgc ggaagtcacg catgatgtac ggcgcctgca gacccaccaa
     4261 ctggcccgga tagaacgcgt tggccgaata ctccggcccg aacgtgtagg gcaccgttgc
     4321 gggatcgatc tggcgcgaaa tgttgccctt ggaaggagcg accttaatat cagtgatatc
     4381 gtagtactcc gatgcgacga cgctcgcctc catggccgcg tccggcccca caatgatgct
     4441 gcgacagacc ttcggcaact ccggcgcccc gcgctccagc atgaccggct cgtgcggcag
     4501 cgacagcacc gtatacggct gcccgtcaat ggtcaccacc gcctgctggt gattcccgat
     4561 ctgatagtcg acgacgattt tgtccggcgt attctcgagc accgtcacgg tgaccggtcc
     4621 ggccccaatc gccggcaggc tggcaaatgc cattgccagc accgcaagcg tcaccctgcg
     4681 actacccatg ctccacatgt cttcactctc ctttttgttg gattgaagcc cgactcaatg
     4741 aggttggtgt gacactactt acccatccgg cgccggcgca ctgccccgca tcagccggcc
     4801 aatgccggcc aaaaccgcac gcaccggctg tcaaaacgat ctcccgcgat gataccagcc
     4861 gttagctgct cgccgcaaga caaataatcg aggtctgcaa tttattcggc ccgttgaacg
     4921 accgcgatga gaattggaat cggaaagata cgactgtaga cagtcttacc gaggcttgtt
     4981 ttcaggcccg ccgggaggcg cgccgcctcg ctcgcctact ccttgttttc tgcgcaagct
     5041 cgtgtcgcgc gccagacccc gaacgcacac aagtccaatc gccaacacga cttatgaacg
     5101 ttgcggcagc gggatgacta cgcttccgac cgtcccgcgg cccgtacgac ggcccttctg
     5161 ggcattcgga gtccgcttca tggttacagc acggctatcc ctagtcctat aaccgtactg
     5221 cggccaccct acccgcctgt gcccgctcaa gccggcacag gctcatcctt cgtgagcgct
     5281 tcgagcacgt atctctcgcc gcgcgcatcc acagaaattt cgtctcccgg cccgaactcg
     5341 ccagccagaa tccgtcgcgc cagtggattc tcaatctgct gctggattag ccgcttcagc
     5401 gggcgagccc cataaaccgg gtcgtatcca tcgctggcca gccggt
//
LOCUS       DAOSYS010000242         5424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12660_length_5424_cov_5.952505, whole genome shotgun sequence.
ACCESSION   DAOSYS010000242 DAOSYS010000000
VERSION     DAOSYS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12660_length_5424_cov_5.952505"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLU99_12960"
     CDS             complement(<1..247)
                     /locus_tag="PLU99_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOO18001.1"
                     /translation="MAAKQIAYEMEAREAIRRGVRQLARAVKVTLGPRGRNVVLEKSF
                     GAPTVTKDGVTVAKEVELEDAYENMGAQMVKEVASKTS"
     gene            complement(677..922)
                     /locus_tag="PLU99_12965"
     CDS             complement(677..922)
                     /locus_tag="PLU99_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18002.1"
                     /translation="MPVAQGFQLERAVADAVSLVNAHSGQTSVTLRFHSAEFGEVDFI
                     AHTASLKGDRFEFSSGFETYDGRLDELANIKAEVIRH"
     gene            complement(926..1222)
                     /gene="groES"
                     /locus_tag="PLU99_12970"
     CDS             complement(926..1222)
                     /gene="groES"
                     /locus_tag="PLU99_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOO18003.1"
                     /translation="MKNVGIRPLGDKVLIKRLEAEETTAGGIVLPDTAKEKPKRGTVL
                     NVGDGKLLDTGERKPLQVKKGDQVLFTSYAGTEIKVGGEEMIIMDEGDILAVLE"
     gene            complement(1700..3112)
                     /gene="glnA"
                     /locus_tag="PLU99_12975"
     CDS             complement(1700..3112)
                     /gene="glnA"
                     /locus_tag="PLU99_12975"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOO18004.1"
                     /translation="MTPEMVLEFIKTNHIEYADLRFLDFPGLWQHLTVPAREVTLDSF
                     KDGFGFDGSSIRGWQAINESDMLIVPVAETMILDPFFQHPTISMICDVKNPLTRAEYS
                     RDPRSVARKAAAYMKNTGIADTAYFGPELEFFIFDKAIYDQGMNFAKYEVDSREGVWR
                     RGRDEPDNLGNQLRAKEGYFPLPPADSMHNLRGEMVAVLEQCGIHVETHHHEVATGGQ
                     AEIDMRYDRLVRMGDKVMTYKYIIKNVAARHGKVVTFMPKPLFGDNGSGMHTHFSLWK
                     GETPLFFGNKYAELSDLALWSIGGLIKHAGSLLAFCCPTTNSYKRLVPGYEAPVNLIY
                     SSGNRSAFVRIPMYQHNPKTKRIEFRCPDSSCNPYLAFSAILMAALDGIQNRIDPGPA
                     VDGNLYDFSPEQLADIGHTPASLDEALRDLDKDHEYLLRGGVFTEDVIRYWIKYKMDC
                     EVAPLRTRPHPYEFCLYFDI"
     gene            3340..3921
                     /gene="aat"
                     /locus_tag="PLU99_12980"
     CDS             3340..3921
                     /gene="aat"
                     /locus_tag="PLU99_12980"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOO18005.1"
                     /translation="MQMPITPELVLAGYRQGIFPMADDDGTIYWFSPDPRTVFEFDRF
                     HVPRTVRQLISRRVFEIRIDTAFDEVISACAAGRRTWISPQIIDLYQQLHRLGFAHSV
                     EAWQGGRLAGGLYGVALGGAFFGESMFFRVSGASKVALVALVERLKERGYTLLDTQWT
                     TPHLARFGAVEIPRRTYLARLRHALTLPCRFVP"
     gene            complement(4051..4914)
                     /locus_tag="PLU99_12985"
     CDS             complement(4051..4914)
                     /locus_tag="PLU99_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOO18006.1"
                     /translation="MPEQQTATRPAQAQKEQPGREHLMHPRPESQARRYKPAGKLAGK
                     TALITGGDSGIGRSVAVMFAKEGADVAIVYLNEHRDADETGRLIEAEGRRCLKIAGDV
                     GRADFCKAAVEQTVREFGKLDILVNNAAEQHVQQTLEDVTEEQLRRTFETNFFGYFLM
                     TQAALKHMKEGGAIINTTSVTAFRGSPHLIDYASTKGAIVAFTRSLAKSLAKRGIRVN
                     AVAPGPIWTPLIPASFPPERVAEFGADVPLGRPGEPDEVAPCFVFLASEDASYMTGQI
                     LHPNGGEIVGS"
     gene            complement(5116..>5424)
                     /locus_tag="PLU99_12990"
     CDS             complement(5116..>5424)
                     /locus_tag="PLU99_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOO18007.1"
                     /translation="VVSPTRRPRRLRDEPWRAEVYHAIKQVMWEKKRIHPNVDYPCGL
                     VYYFLGLPPDIYTPLFVMARVSGWCAHIIEQHEHNRLIRPRSKYTGPPRRPWQPLAAR
                     "
BASE COUNT          962 a         1713 c         1631 g         1118 t
ORIGIN      
        1 tgctggtctt ggaagctact tccttgacca tctgggcgcc catgttctca tacgcgtctt
       61 ccagctcgac ctctttcgcg actgtcacgc cgtctttcgt caccgtcggc gcgccgaacg
      121 atttttcgag aacgacgttg cggccgcgcg gtccaagcgt caccttcacg gcccgggcga
      181 gttggcgtac tccgcgccga atcgcctcac gggcctccat ctcatacgca atttgctttg
      241 ctgccatcga cctctcctaa ctaggccgtc tctgtcggcg ggtcgaagcc gccgacgctg
      301 tccgaattca tcagaaactt gtggaccggg tttcaaccgg tctgacacac ttgtggagcc
      361 ggctctgaac cggtcgccac atccgggacc ggtttcaacc cggtcgacca cctcccagcc
      421 ccactggccc acgccggcca acaacgaaca aaaccttccc gccggcccgg cggcattcaa
      481 gttccgttcc aaatgtttcg caggaatgag ccgactaccc gtaagtggcc tgaaatcagg
      541 tacttacaac tcggaaaggc acgccccaaa ccggccaatc cactcatgcc ctatgtcaaa
      601 ctggcatact ggcaaccaag cgaaagagtt ctcgcccggc ctgtcagcct ttgaagtcag
      661 cacgcaggca gcgccatcag tgacgaatta cctcggcttt gatgtttgcc agctcgtcga
      721 gccggccgtc atacgtctca aatcccgagg aaaactcgaa ccgatcaccc ttcaagctgg
      781 ccgtatgtgc gatgaaatct acttcgccga actcggccga gtggaaccgt agggtgacgc
      841 tggtctggcc tgaatgtgca ttcaccagac tgaccgcgtc agccacggcg cgctcaagtt
      901 ggaagccctg tgccaccggc atgtactact ccagtacagc gagtatatcg ccctcgtcca
      961 taatgatcat ttcttcgccg cccaccttga tttcggtgcc ggcgtagctt gtgaacagaa
     1021 cttggtcccc cttctttacc tgtagcggct tccgctcgcc ggtgtccaaa agcttcccat
     1081 caccgacgtt gagaacggtt ccgcgcttgg gtttttcctt ggcggtgtca ggcaacacga
     1141 tcccgccggc ggtggtttcc tccgcctcaa gccgtttgat cagcaccttg tccccgagcg
     1201 gacgaatccc gacattcttc atgcgtgtcg ctccaattct atattttgcc tcattaagcc
     1261 acgcgggtca aaacaatttt gcgcgcgcag cagttctgct gctggccgcc tcagcaatca
     1321 acatgccact gggcggcgcg catggcggtt tcgctcacag tgcgtaaacc ggccccagat
     1381 agacacttac gcgcctggtg cgggccggca aacatgcccg tgaatagcat gtcacccaca
     1441 cgcgggcacg atcaaaattg cacaactgtc aacttggcag ccccgcgaaa ccgcccgagc
     1501 caacggaaac gcggccggcg gatgccgttg gaatgccgct ccggggtgcc gccgcgtgcc
     1561 ggcgggcgcc ggcgcgccga tctcgccaac ggcaatcgca aatccttaat gacgatacgc
     1621 tgccacgata gggcgagccc ccgcgggcct cgggtccgcg ggggcgtcgc cctcccggtc
     1681 gtgccttaca tgtcgtgact tagatgtcga aatacaggca gaactcgtac gggtgcgggc
     1741 gcgtacggag cggcgccact tcgcagtcca tcttgtactt gatccagtag cggatcacgt
     1801 cctccgtaaa cacgccgccg cgcagcaggt attcgtggtc cttgtccagg tcgcgcagcg
     1861 cttcgtccag cgaggccggt gtgtggccaa tgtccgccag ctgctccggc gagaaatcgt
     1921 acaggttacc gtccaccgcc ggccccggat cgatccggtt ctgaattcca tcgagggccg
     1981 ccatgagaat cgcgctgaac gccaggtacg ggttgcagct cgaatccggg cagcggaact
     2041 cgatccgctt ggtcttgggg ttgtgctgat acatcggtat gcgcacgaaa gctgagcgat
     2101 tccctgacga gtaaatcaga ttgacggggg cctcgtagcc cggcaccagc cgcttgtagc
     2161 tgttggtggt cgggcagcag aacgccagca ggctgccggc gtgcttgatc aggccgccga
     2221 tggaccacag cgccaggtcg gacagctcgg cgtacttgtt tccgaagaac agcggcgttt
     2281 cgcctttcca cagcgagaag tgcgtgtgca tcccgctgcc gttgtcgccg aacagtggct
     2341 tcggcatgaa cgttacgact ttgccgtgcc gcgccgcgac gttctttatg atgtacttgt
     2401 aggtcatgac cttgtcgccc atgcgcacca gccggtcgta acgcatgtcg atctcggctt
     2461 gcccgccggt ggcgacctcg tgatggtgcg tctcgacgtg aattccgcac tgctcaagca
     2521 cggccaccat ctcgccgcgc agattgtgca tcgaatcggc cggcggcagg gggaagtaac
     2581 cctctttcgc ccgcaattgg ttgcccagat tgtccggctc gtcgcgcccg cgccgccaca
     2641 cgccctcgcg cgaatcgacc tcgtacttgg cgaagttcat gccctggtca tagatcgcct
     2701 tgtcgaagat gaaaaattcc aactccggcc cgaagtaggc cgtgtcagcg atgcccgtgt
     2761 tcttcatgta ggcggccgct ttgcgcgcca ccgaacgcgg atcgcgcgag tactcggctc
     2821 gcgtgagcgg gttcttaacg tcgcaaatca ttgagatagt cgggtgctgg aaaaacgggt
     2881 ccagaatcat cgtctcggcc acgggcacga tgagcatgtc cgattcgttt atcgcctgcc
     2941 agccgcgaat ggacgaaccg tcaaagccga acccgtcctt gaacgaatcc agcgtcacct
     3001 cccgggccgg aacagtcaga tgctgccaaa gcccgggaaa atccaaaaac ctcaaatctg
     3061 cgtactcaat gtgattggtt ttgatgaatt cgagcaccat ttcgggcgtc atgccgaatc
     3121 tccgtgggtt tgttgtgaca actagggggc tccgaacgaa gggcgatagc ttacaagccg
     3181 cgccgcccgg ctacaactgc ccgctgtcgc ccctttccgg tttttagcgc cggcttggcg
     3241 gacttgcata acgggctcaa acggcccatc ctaagagtgg caggctccga ccgatgttcc
     3301 ggagaaactt atcgataatt ccctcccgcg acacgtgcca tgcagatgcc catcacccct
     3361 gaattggttc tcgcgggtta ccgccagggc attttcccta tggccgacga cgatggcacg
     3421 atctactggt tcagccccga cccgcgcacg gttttcgagt tcgaccgctt ccacgtgccg
     3481 cgtaccgtgc ggcaacttat cagccgaagg gtgttcgaaa tccggatcga cacggccttt
     3541 gacgaggtaa tctcggcctg cgccgccggc cgccgcacct ggatttcacc gcagataatc
     3601 gatctttatc agcaacttca tcggctcggc tttgcgcaca gcgtcgaagc ctggcagggg
     3661 gggcgattgg ccggcgggct ttacggcgtc gcgttgggcg gggcgttttt cggcgaatcg
     3721 atgttcttcc gcgtaagcgg ggcttcgaag gtggcgctgg tggcactggt cgagcggcta
     3781 aaagagcggg gctacacgct gctcgacacg cagtggacca cgccgcacct ggctcgcttc
     3841 ggcgcggtcg agataccgcg tcgcacgtac ctcgctcgac ttcggcacgc cttgacgttg
     3901 ccgtgccggt ttgttccgta gtggccgcat gttcggatga gcgactaatc gagaaggctc
     3961 gccactacat ggagcgttct cgctagatcg tataattggg gacagtcact gggactgtcc
     4021 ccagttagag ggctgtcggc gatggttcat tcagcttccc acgatctcgc cgccgttggg
     4081 gtgaaggatc tgacccgtca tgtaggacgc gtcctcgctg gctagaaaga cgaagcacgg
     4141 tgcgacctcg tccggctcgc ccggccggcc gagaggcacg tccgccccga actccgccac
     4201 ccgctcgggc ggaaagctgg cgggtatcag cggcgtccaa atggggccgg gcgccacggc
     4261 gttgacgcgg atgccgcgct tggccagtga cttcgccagc gagcgggtga acgcaacgat
     4321 ggcgcccttg gtcgaggcgt agtcgatcag gtgcggactg ccgcggaaag ccgtcacgga
     4381 agtcgtattg atgatcgcgc cgccttcttt catgtgcttc aaggcggcct gggtcatcag
     4441 gaagtagccg aagaagtttg tttcaaaagt gcgccggagc tgctcttcag tgacatcctc
     4501 cagtgtctgc tggacgtgct gttcagcggc gttattaaca agaatgtcga gcttgccgaa
     4561 ttcgcgcacg gtttgctcga ccgccgcttt gcagaagtcg gcgcggccca cgtcgccggc
     4621 aatcttcagg cagcggcggc cctcagcctc gatcagccgg ccggtttcat ccgcgtcccg
     4681 gtgctcattg aggtagacga tggccacgtc cgctccctcc ttggcgaaca tgacggcgac
     4741 gctgcggccg atgccgctgt ccccgccggt tatcaaggcg gtcttgcccg cgagcttgcc
     4801 ggctggctta tagcgtcggg cctgcgactc gggtcgtggg tgcatcaggt gttcgcggcc
     4861 gggctgttcc ttttgggcct gcgctgggcg cgttgcagtt tgttgttctg gcattttgtc
     4921 ggtcctttat gaaggtgtct ggctcgcgac ttgttgttcc gcgacgagcg gattgcggtc
     4981 gacgtcgcag tatggtcgcc gcccatgaca gtgacctgac tggtgagtga aatgccagtc
     5041 acggccccaa ggagcgcgag acagcgcggc cggcgcgctg ggcgcgcgcc ggcgtttttg
     5101 agcttggacg gatgtttacc gcgcggccag cggctgccac ggtcgccgcg gcgggccggt
     5161 gtatttgctg cgcgggcgga tcaggcggtt gtgctcatgc tgctcgatga tgtgcgcgca
     5221 ccagccgctg acgcgcgcca tgacgaacag gggcgtgtag atatccggcg gcaggccgag
     5281 gaagtagtag accaggccgc aggggtagtc gacgttcggg tgaatccgct ttttctccca
     5341 catcacctgc ttgatggcgt ggtacacctc ggcccgccaa ggctcgtcac ggagccgccg
     5401 cggccgcctc gtcggagata cgac
//
LOCUS       DAOSYS010000243         5421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12671_length_5421_cov_4.999068, whole genome shotgun sequence.
ACCESSION   DAOSYS010000243 DAOSYS010000000
VERSION     DAOSYS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12671_length_5421_cov_4.999068"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1370
                     /locus_tag="PLU99_12995"
     CDS             75..1370
                     /locus_tag="PLU99_12995"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HOO18008.1"
                     /translation="MLHTARRKAAGVFRTLGSLLPGRGKRARRTTRVLRPWWRDSHQN
                     YPTIGLTPSRLMSYLQAADAGAPQMQFELFSEMLQKWPRLAAVENTRRLALTGLDWEL
                     RPATSVVGGAGNGVAQAAADYCREVLGGLERFRQALSHLANAIGYGVAVAELVWESGR
                     LVDIVPVPYTRLCADPREPWRIRVLTEDDGARGVALDEQPVKWIVHQPRAVPGRPFEG
                     GLLRASALLYLAQNLSFKEWLIYSQIAGMPLRLAQFEPGTPEAEKQNLIRMLEALGTD
                     AVAACSKSIDLKFIEPARQQGPYEALQNYCNTEVTILWLGQHLTTDIRSSGSRAAAEV
                     HDRVREDLLVHDIAEEAATIERDLLTPLVRARFGPQAPVPSFGRALIQSVDTKVLAET
                     LAVAVDHLGLEVPRSWLYRALGVPEPEPGAPVLTRRGSQ"
     gene            1367..1924
                     /locus_tag="PLU99_13000"
     CDS             1367..1924
                     /locus_tag="PLU99_13000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO18009.1"
                     /translation="MRQPKSRRLSTTQALLDTRTSQLTVRPARECDLVPLTFFVDAVL
                     RKDYFLRRGQLASMLRDRRHEILIAELDGVLVGVAILTRGAHLVNLLVHPAYRRLGIG
                     RCLVERSQAQSVSVKLDMSSGDPRAFYEALGFESTGRRNRKGNIERMQLIGSGRDRAE
                     NCGRPGVTAPSAVETSQVKAGNAAQ"
     gene            1940..3097
                     /locus_tag="PLU99_13005"
     CDS             1940..3097
                     /locus_tag="PLU99_13005"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HOO18010.1"
                     /translation="MSTSEVVEKASSRSPLDEVARQQIMRRLQEAVELAEEDLGRHLG
                     QFQGVLQDLVLRSQALEVAHSAVDRSTVEPVCNRSISHVLACSELTARPGEPLREFLL
                     IPFGEVKVERPCAGGSFVFTPAHAESARRWFEGIGRKLAIDYEHQSFDRFNTRPDGLR
                     PAAGWIGGLEVRDDGLWATDITWTERAAELLRSGEYRYFSPVIFWTDEDQTDVAGLGP
                     VALTNDPAMRGVMPLAARCAAGAVDEADEAEPSQVGGAVEGAGNLAAELTAAREEIAL
                     LKRQLAIQAADAFVERGLRQGKIVEGTATDWRQEYMRDPELAEQRLALAAVVLPPGRV
                     MPLDRRGQVEPQRVSARAPGMSNPLAIAAEDLEAYERAVLTGRVLRAGGAR"
     gene            3151..3888
                     /locus_tag="PLU99_13010"
     CDS             3151..3888
                     /locus_tag="PLU99_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18011.1"
                     /translation="MALVENRELAFYTSQELIDLPVDDNVKIFKGALVGRNRSTGFAR
                     GLVAGDEFLGIAYQQADNTGAGHAAGAIRVRLHQNVDVVHALTGVVTGDIGRDVYATD
                     DGTLTLAPTGNSRVGRVVAVEAANLARVRCQPVAGVAGVIENWPVVVLPDANLTLSLD
                     HMNRVLLIGNSAARTLTLPPVATVRAGGWFRIVKTSAAAFAVTLDANGAETIDGNATY
                     AAIDAKYDTALLMCTGTEWIIVSRDVA"
     gene            3945..4868
                     /locus_tag="PLU99_13015"
     CDS             3945..4868
                     /locus_tag="PLU99_13015"
                     /inference="COORDINATES: protein motif:HMM:NF021628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HOO18012.1"
                     /translation="MAVVDTGLTSKAIIGRFYRRLEEFAQNAWWTKLAMHFTSTQESE
                     TYRWLGMVPQVREWIGGRQVRPLRSQGVTIVNKTWESTIRVDADEQRRDKTGQVLVRI
                     NEMARRVATHPNSLLTSLMLSGETSACYDGHNFFSATHSEGDSGTQSNSITFDAVTPT
                     APTDTEMYEAIVKSIGQLLSLKDDQGEPMNEAARQFVVMVPTSFWSATTIALSDATRD
                     RVNPLALGRPYEIDWVANPRLTWTTKFAIFRTDGETRPFIFQEELPVQVQALAEGSEL
                     EINENQHQYGVKAVHAAGYGFWQDACLVTFN"
     gene            4994..>5421
                     /locus_tag="PLU99_13020"
     CDS             4994..>5421
                     /locus_tag="PLU99_13020"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HOO18013.1"
                     /translation="MAYITAADLSRRLGATLYARLTDRENGTTANAAVAETIVAEAES
                     EANSYLAARYATPVSLSVHPELADVLARRVLDLAEYGAWRGSPFVSDIPARVKALYLE
                     ACAWFSRVAAGAPLPASSAPAATELVDDTPVYRAQPRTFTA"
BASE COUNT          980 a         1587 c         1954 g          900 t
ORIGIN      
        1 aatcctatca catggccggc taggctcttg acgtgaagcg cggcggccgc gatcggccgc
       61 ccaaggagaa acgcgtgttg cacactgctc gcagaaaagc cgccggggtg ttcagaacgc
      121 tcgggtcgtt gctgcccggg cgcgggaaaa gggcgcgacg aacgacgcgc gtgctccggc
      181 cctggtggcg cgacagccac cagaactacc ccaccatcgg gctcacgccg tcccggctga
      241 tgtcctatct ccaggcggcc gacgccgggg cgccgcagat gcaattcgaa ctgttcagcg
      301 agatgctcca gaaatggcca cgcctggcgg ccgtcgagaa cacccggcga ctggcactga
      361 cgggactgga ctgggaactg cggccggcca ccagcgtggt tggcggtgcc ggcaatggcg
      421 tcgcgcaagc cgcggcggac tactgccggg aagtcttggg cggtttggag cgcttccgcc
      481 aggcgctcag tcatctggcc aatgccatcg gttacggcgt cgcagtggcg gaactggtat
      541 gggaaagcgg gcggctggtc gacatcgtgc ctgttcctta tacgcgtctg tgcgccgatc
      601 cgcgggagcc gtggcgcata cgggtgctga cggaggacga cggggcgcgc ggcgtggccc
      661 tggatgagca gccggtcaag tggatcgtgc accagccgcg ggccgtgccg gggcggccct
      721 ttgagggcgg actgttacgg gcctcagcgc tgctctatct ggcgcagaac ctcagtttca
      781 aggagtggct gatctattcg cagatcgccg gcatgccgct gcgcttggcg cagtttgagc
      841 cgggcacgcc cgaggcggag aagcagaacc tcattcgcat gctggaggcg ctggggacgg
      901 acgcggtggc ggcctgcagc aagagcattg atttgaagtt cattgagccg gcgcggcaac
      961 agggacccta cgaagcattg cagaactact gtaatacgga agtcaccatt ttatggctgg
     1021 ggcagcacct gactacggat attcgcagtt ccggttcgcg ggccgcggcc gaggtgcatg
     1081 accgcgtgcg cgaggatctg ctggtacacg acatagccga ggaggccgcc accattgagc
     1141 gtgacttgtt gacgccgctg gtgcgggcgc gctttgggcc gcaggcgccg gttccgtcct
     1201 tcggccgggc cctgatccaa tcggtggaca cgaaagtgct ggccgagacg ttggcggtcg
     1261 cggtggacca tctggggctc gaagtgccgc gcagttggct gtaccgggcg ttgggggtgc
     1321 cggagccgga gcccggcgcg ccggttctga cgcggagggg atcgcaatga gacagcccaa
     1381 gagccgccgc ctcagcacca cacaggcgct gctggacacg aggacgtccc agttgacagt
     1441 gcgtccggcg cgcgagtgcg acttggtccc gctcacgttc ttcgtggatg cggtgctgcg
     1501 gaaggactat ttcctgcggc gcggccagct cgcgagcatg ctgcgcgacc ggcggcacga
     1561 aatcctgatc gccgaactgg acggcgtttt ggtgggcgtg gcgatcctga cgcgtggcgc
     1621 gcacctcgtg aacctgctgg tgcatccggc ttatcgccgg ctgggaattg gacgctgcct
     1681 ggtcgagcgc agccaggcgc aatcggtgag cgtcaagctc gacatgagca gcggcgaccc
     1741 gcgcgccttc tacgaagcgc tcgggttcga gtctaccggg cggcgaaatc gcaaaggcaa
     1801 catcgaacgg atgcaactga ttgggtccgg gcgcgaccgg gcagaaaact gcggccgccc
     1861 gggtgttacc gcgcccagcg cagttgagac aagtcaagtc aaagccggca atgcggcaca
     1921 gtgaaagtga ggtataccct tgagcacaag tgaagtcgtg gaaaaggcga gcagtcgctc
     1981 gccattggac gaagtggccc ggcagcagat catgaggcgg ctgcaggaag cggttgaact
     2041 ggccgaagag gacctggggc ggcatttggg gcagtttcaa ggagtgctgc aggacctggt
     2101 gctgcgcagt caagcgctgg aggtggcgca cagtgctgtg gatcgctcta ctgtggaacc
     2161 ggtgtgcaac cggtctatca gccacgtgct ggcgtgcagc gagctgacgg cgcgccccgg
     2221 agagccgctg cgcgagttcc ttttgattcc attcggcgag gtcaaagtgg agcggccctg
     2281 tgcgggcggc agctttgttt tcacgccggc ccacgccgag tcggcccggc gctggtttga
     2341 aggcatcggc cgcaagctgg ccatcgacta tgaacaccag agtttcgacc gcttcaacac
     2401 gcggccggac gggctgcggc ctgcggccgg ctggatcggc ggtctggaag tgcgggacga
     2461 cgggctgtgg gcgacggaca tcacctggac ggagcgggcg gcggaactgc tgcgatcggg
     2521 tgagtaccgc tacttctcgc cggtgatttt ctggacggat gaggaccaga cggacgtggc
     2581 ggggctggga ccggtggcgt tgaccaatga tccggccatg cgcggcgtga tgcctctggc
     2641 ggctcggtgc gcggccggcg ccgtggatga ggcggacgag gcagagccgt cgcaggtggg
     2701 tggggcggtt gaaggagccg gcaatctcgc ggccgagttg actgcggccc gcgaagagat
     2761 cgcccttctg aaacgtcaat tggcgattca agcggcggat gcatttgtcg agcgcggcct
     2821 gcggcaggga aagatcgtgg aaggcactgc tacggactgg cggcaggaat acatgcgtga
     2881 cccggagctg gccgagcagc gactggcgct ggcggctgtg gtgttgccac cggggcgggt
     2941 catgccgctg gaccggcgcg gccaggtcga gccgcagcgc gtcagtgcca gagcgcctgg
     3001 gatgagtaac cccctggcaa ttgcggcgga ggatctggaa gcctacgagc gcgcggtgct
     3061 gacggggcgt gtgttgcggg caggcggtgc ccgttagcga cgggaactgt gggctgacca
     3121 gcgaatgaac tttcttcagg agagattaac atggccttgg tagaaaatcg tgaactggcg
     3181 ttttacacgt cacaagagct gatcgacctg ccggtcgatg acaacgtgaa gatcttcaag
     3241 ggggcattgg tggggcgcaa ccgctctacg ggtttcgccc gcggcctggt ggccggtgat
     3301 gaattcctgg gcattgctta ccagcaggcc gacaacaccg gggcggggca tgccgccggc
     3361 gctataaggg tgcgcctgca ccagaacgtg gacgtggtgc atgcgctgac gggcgtggtg
     3421 acgggcgaca ttgggcggga tgtctatgcc acggacgatg gaacgctgac gctggcgcca
     3481 acgggtaaca gccgcgtggg gcgcgtggtg gcggttgaag cggccaatct ggcgcgggtg
     3541 cgctgccagc cggtggcggg cgtagccggc gtcatcgaga actggccggt ggtcgtactc
     3601 ccggacgcca acctcacgct tagcctggat cacatgaacc gggtgctgct gatcggcaac
     3661 agcgcggcgc gcacgctgac gctaccgccg gtggcgaccg tgcgagcggg cggctggttc
     3721 cgcattgtga agacctcggc ggcggccttc gcagtcacgc tggacgccaa cggggcggaa
     3781 accatcgatg gcaacgctac ctacgcggca atcgacgcga aatatgacac ggcgctgctg
     3841 atgtgtacgg ggaccgaatg gatcatcgtc agccgggatg tcgcgtaata gctgaagcgc
     3901 ggccggctgt ggtgcaagtc aagacttgaa tctgggagac attcatggct gttgtagaca
     3961 cgggcctgac gtcgaaggcc atcattgggc gcttttaccg gcggctggaa gagttcgcgc
     4021 aaaatgcctg gtggaccaag ctggcgatgc acttcaccag cacgcaggag tcggagacgt
     4081 accgctggct gggaatggtg ccgcaggtgc gcgagtggat cggtgggcgc caggtgcgtc
     4141 cgctgcgctc gcagggcgtg accattgtca acaagacgtg ggaatccacc attcgcgtcg
     4201 atgccgatga gcagcggcgc gacaagaccg gccaggtgct ggtgcgcatc aacgagatgg
     4261 cgcggcgcgt ggcgacgcac cccaactcgc tgctgaccag cctgatgctg agcggcgaaa
     4321 cgagcgcgtg ctacgacggg cacaacttct tctccgcgac gcacagcgaa ggcgactcgg
     4381 gcacgcagag caactcgatc acgtttgacg cggtcacgcc caccgccccg accgacacgg
     4441 agatgtacga agccatcgtc aagtccatcg ggcagcttct gagtctgaaa gacgaccagg
     4501 gcgagccgat gaacgaggcg gcccggcagt ttgtggtcat ggtgccgacg agcttctggt
     4561 cggccacgac cattgctctg tccgacgcca cccgcgaccg ggtcaacccg ctggcgctcg
     4621 gccggccgta cgaaattgac tgggtggcca acccgcggct gacgtggacg accaagttcg
     4681 ccatcttccg cacggacggc gaaacgcggc cgttcatctt ccaggaggaa ctgccggtgc
     4741 aggtgcaggc gctggcggaa ggcagcgagc tggagatcaa cgagaaccag caccagtacg
     4801 gcgtgaaggc ggttcacgcg gccggttacg gcttctggca agacgcctgt ctggtgacgt
     4861 tcaactagca gcgctgaggc gaaccgggat ttttggggag ccggagtggt ggcggagtcc
     4921 ggtggggtcc ggactccgcg ccgctcaact tgagcatagc tcaattcaag aaattcgaga
     4981 aaaaggaata ccagtggcct acatcaccgc agcagatttg agtcgccggc ttggggcgac
     5041 gttatacgcg aggttgactg atcgcgaaaa cggaacgact gccaacgccg cagtggcgga
     5101 gactatcgtc gccgaggccg aatcagaagc caacagttac ctcgcggcgc gctacgccac
     5161 tccagtgagt cttagcgttc atccggagct ggccgatgtg ttggcgcggc gcgtactgga
     5221 cttggcggaa tatggtgctt ggcgcggctc accattcgtc agcgacattc ctgcccgcgt
     5281 caaagcgctc tacctcgagg catgcgcttg gttcagccga gtggcggccg gtgccccgct
     5341 gccggccagc agcgcgcccg ctgcgaccga gctggtcgac gacacaccgg tctaccgcgc
     5401 ccagccgcgc accttcacgg c
//
LOCUS       DAOSYS010000244         5370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12806_length_5370_cov_5.666228, whole genome shotgun sequence.
ACCESSION   DAOSYS010000244 DAOSYS010000000
VERSION     DAOSYS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12806_length_5370_cov_5.666228"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..523)
                     /locus_tag="PLU99_13025"
     CDS             complement(212..523)
                     /locus_tag="PLU99_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18014.1"
                     /translation="MSENEVAGGPDTNPACAASGPATTPEDNERTASRERRRQNMKAL
                     TASEAADARSYIETEASRFDAENPNLPPPRIYDLVTVRQIKRQPWLIENILPACSLGL
                     L"
     gene            1173..2285
                     /locus_tag="PLU99_13030"
     CDS             1173..2285
                     /locus_tag="PLU99_13030"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOO18015.1"
                     /translation="MTRTFASLALCAVILVTAAWAQAPDRPKQPQQPGDRPQMGQPQQ
                     PGAQQQTGQQQTGQQMGQAGQRQTGAAGQLQPPSAWYKAKDLIGKHLRDANNTELGEI
                     DDLIMLPHDGAIPFAIMSGRRIDRAGEHIALPWSVLSFEPGAKDVTLNTTKDKLQAAQ
                     LAAFKGDQWPDFALATFVERVYREFGESPPAYMQQVRTAGQVEREGGQAAAGAHWFKI
                     STLRDDEIRSADGTKIGDVDNVVIDLNNGHILFVTLAGRHVDRHDEYVAIPWGALHIE
                     GSDDRTHLVLNMSKEQLKTAPAFKEDNWANITDTAFLKNVFAFYKVQPYWEGRTRQAG
                     YTEPTKQRDTGDTGDTGERGQQPGGGQQPGGGGRQP"
     gene            2767..3513
                     /locus_tag="PLU99_13035"
     CDS             2767..3513
                     /locus_tag="PLU99_13035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO18016.1"
                     /translation="MDIPRIFNITESAHRIHNPITPDKLATLGAALRLEPGARVLDLG
                     SGSGEMLCTWARDHGITGTGVDLSQLFTEQAKRRAEELGVADRVKFIHGDAAGYVSDE
                     KVDVAACVGATWIGGGVAGTIELLARSLRSGGIILIGEPYWRQLPPTEEVAKACFAHS
                     ISDFLMLPELLASFGRLGYDVVEMVLADQDGWDRYEAAKWLTMRRWLEANPDDEMAKE
                     VRAQLTSEPERYAAYTREYLGWGVFALMRR"
     gene            3680..3865
                     /locus_tag="PLU99_13040"
     CDS             3680..3865
                     /locus_tag="PLU99_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18017.1"
                     /translation="MENEKDSDMLIRNWQKWAAVLACGAALLQVPSCTDAATVVTSIA
                     STVTAGGVIYIIARIME"
     gene            4121..4876
                     /locus_tag="PLU99_13045"
     CDS             4121..4876
                     /locus_tag="PLU99_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO18018.1"
                     /translation="MLRSWIACRPASYAPFGYLAYDHLAGLGIRFVEIPVEAARAVAA
                     ELGRRNLQISSLQGEFDVARDDVPGQLKPQLQTCNLFGVRLLFLSVKAGHTPLGTVYD
                     RLRAAGDLAARQGVTLALETHPDLVTNAQVARQTMEGVDHAHVRVNFDTANVYFYNHG
                     VDAVEELRRMLPFVASVHLKDTDGGFRSWHFPALGRGIVNFRGVFEVLDQAGFTGPCT
                     LEIEGIEGEEKTERLVCDRMAESIGYLRGLGRL"
BASE COUNT         1014 a         1739 c         1688 g          929 t
ORIGIN      
        1 cgatgaacag cggcccgtca tcgatgtttt ggcccgcgag ccccttatcg cgcagccagc
       61 ccccaacacg agcacccagg ccgttcgcgc cctcggcggt caggtagacg accgggcctt
      121 gcctcgtggg atggcctaaa aagtcaatgc ccagtgcgac ggaaccggcg atatccagcg
      181 cagtgaatga cttgcccgtg ccttccttgc cctacagcaa gccaagggag caagcgggca
      241 gaatgttctc gatgagccaa ggctggcgct taatctgcct tacggtaacg aggtcgtaga
      301 tgcgcggtgg gggcaggttc gggttctcgg catcgaagcg gctggcttct gtttcaatat
      361 aggaccgagc gtctgctgcc tcgctggctg ttagcgcctt catgttctga cgacggcgct
      421 ctctgcttgc tgttctttcg ttgtcttcgg gcgtcgtagc cggtccgctc gctgcgcacg
      481 cgggattcgt gtccggacca ccagcaactt cattttccga catacacttg cctttcagat
      541 agcaaagacc ggctcgctcc ctctgcgtct ggtgtgtcct gcggacatag taccgtccct
      601 ttgcagtctc agcaaaagct gcgttttcct ggaaccgcca gtggatgaga ccgcactcgc
      661 acggttgtta tcggcccccg ttcgcgcatc gcgtgccagg gcgagaggat atgcactgac
      721 gcattgcggc gcgggggcat caggtgaggg cgcggccagg tgctggaccg cccgcgcccc
      781 cgctgagccc gagcgcctat ctcttcgatt aacgagtaac tagccggaga gaggccgatc
      841 gtccggcgga tcgccaaacc ctacgagcgg cccgcgctca cgcttcgggc tcggattccc
      901 tgcctgtgat ctggactcgc tactgcctgg gtctatgggg cccacttcgc aatcctgcgc
      961 cggccgctcg tggaagagca atcgccgccg ccctgatctg ggcgacgccc gcttataacg
     1021 ctgatttagg acaattagga caacctcgcc cgggcttgaa ccctatttca gccgtttctc
     1081 agcccactgt caggactgcc gcgctatgta tgtatttggc ggccgagccc ttgtaatgaa
     1141 ccgccggtta cggacccaaa aaggagagca cgatgacccg aacgttcgcc agtctggcac
     1201 tttgtgccgt cattcttgtc actgcggcct gggcacaagc ccccgaccgc ccaaaacagc
     1261 cgcaacagcc gggcgaccgg ccgcaaatgg gacagcccca gcaacccggg gcacagcaac
     1321 agaccggtca gcagcagact ggtcagcaga tgggacaggc aggtcaacgt cagaccggcg
     1381 ccgctggtca actccagccg ccgtcggcct ggtataaagc caaagacctc atcggcaagc
     1441 atctcagaga tgccaacaat actgaactcg gcgaaatcga tgacttgata atgctgccgc
     1501 atgacggggc catacccttc gcgatcatgt caggccggcg catcgaccgg gccggcgagc
     1561 acattgcgct gccctggagc gtgctgagct tcgaaccggg cgccaaagac gtgacgctga
     1621 ataccaccaa ggacaagcta caggctgcac aactggcggc attcaagggt gaccagtggc
     1681 ccgatttcgc cctcgcgacg ttcgtcgagc gcgtctaccg cgaattcggc gaaagcccgc
     1741 cggcgtacat gcagcaggtg cgcacagccg gccaggtgga gcgcgaaggt ggtcaagcgg
     1801 ccgccggcgc ccattggttc aagatcagca cgctgcgaga cgacgagatc cgcagtgcag
     1861 acggcaccaa gatcggcgac gtcgataatg tggtcatcga tcttaacaat ggccacatac
     1921 tgttcgtcac tctggccggc cggcatgtcg atcgccacga cgaatacgtg gccatcccct
     1981 ggggcgcgct gcacattgag ggcagcgacg accggaccca ccttgtactg aacatgagca
     2041 aggaacaact gaagaccgcg ccggccttca aagaggataa ctgggcgaac attacggaca
     2101 cggccttcct gaagaacgtt ttcgccttct acaaggtgca gccgtactgg gaaggccgga
     2161 cgcgccaggc cggttacacc gaaccgacta agcagcgcga caccggcgac accggggata
     2221 ccggcgagcg cggtcagcag cctggcggcg gtcagcagcc tggcggcggc ggccggcagc
     2281 cttaatcgag cggcctccag cgggcgcgca gccggcggcg tgctgcgggc cacacagacg
     2341 gcagactgat tcaaggcgcg gcgctgcgaa gcgccgcgcc gcgggtcagt ctttgtcgtt
     2401 tttgacgcct gtctccccta gaagcccggc gtggcgatgg tcgggcaagt ggcgatgatt
     2461 ttgacaaatc gccgcgcccg ccgtccccaa agacaggtga gcaggcgaac aggtgaacag
     2521 gagcgggcgg cggctgcgcg gcgtcgcggc ccacgcaact cgaacgtttc ttgcgggtcc
     2581 aatgctctga ccgcagtgcg gctcagtcat tccaagggcg cggacgaggc attcgaaatc
     2641 tcttcccgaa tcgccaaaca aggttgtcaa accaaccatt tcggcgataa tgagcgccaa
     2701 attggaaaat aataacttgc taagccgtaa cccaatccat tcgcacccgt aagaaaagtt
     2761 gccacagtgg acatcccacg catatttaac atcactgaga gtgcgcaccg catccacaac
     2821 ccgatcacac ccgacaagct cgccacgctc ggcgcggcgc tgcgcctgga accgggcgcc
     2881 cgcgtgctcg acctcggcag cggttcgggc gagatgctgt gcacttgggc gcgcgatcac
     2941 ggcatcaccg gcaccggcgt cgacctgagc cagttgttca ccgagcaggc caaacgccga
     3001 gcggaggaac tgggcgtcgc cgatcgcgtc aagttcatcc atggcgacgc ggccggctac
     3061 gtttctgacg agaaggtcga tgtggcagcc tgtgtcggcg ccacctggat cggcggaggt
     3121 gtcgccggca cgatcgagct tctggcgcgc agcctgcgca gcggcgggat catcctcatc
     3181 ggcgagccgt actggcgcca gttgccgccg acggaagagg ttgccaaggc gtgttttgcc
     3241 cactccatct ctgactttct catgcttccg gaactgctcg cgtctttcgg ccgcctgggc
     3301 tacgacgtcg ttgaaatggt tctggctgac caggacggct gggacaggta tgaggcggcc
     3361 aagtggctaa ccatgcgccg ctggcttgaa gccaatcctg acgacgagat ggcaaaagaa
     3421 gtgcgagccc aactgacttc ggaacccgag cgctatgccg cttacacgcg cgaatacctg
     3481 ggctggggcg tgttcgcgct gatgcggcgt tgacgcacgc gcagatcaga gccggggcgt
     3541 gtcgccccgg ctctgctccg cccccgcgcc ggcgacgccg ggctgttgac gagaggcgcg
     3601 aatcggggca tagtccacgc tcggcgcggt gaagcctcga gacgcgcggg cgggtgccgg
     3661 cgcgcttcag caatcccgcg tggagaacga gaaggattcc gacatgctga ttcgcaactg
     3721 gcaaaaatgg gcggccgtcc ttgcctgcgg cgccgccttg ctccaagttc caagctgcac
     3781 ggatgcggcg acggtcgtca ccagcatcgc gagcaccgtc acggcgggcg gcgtgatcta
     3841 cataatcgcc aggattatgg aatagtcgcg ggaccggccg atgccggcgt gatgtgtggt
     3901 tgggctgttc cttcggcgtc gctggcggcg aattgcgccg gccgatgccg ggcgtgatgt
     3961 gtcggccgat gttggcgtga cgtggggatt gcggttcagg accgccgcga aactgcgcaa
     4021 aagactgcca cgaaactgcg caaagcgcct gctgctgtgc cgccggcttc aggccctcat
     4081 gtcattgccg gtgtgccata actttgccag cgggtgacct atgctacgaa gctggattgc
     4141 atgccgtccg gccagctacg cccccttcgg ttacttggct tatgaccatc tggccgggct
     4201 gggaatccgt tttgtcgaaa tcccggtgga ggcggcccgc gcggttgcgg ccgagctcgg
     4261 ccgtcgcaac ctgcagatca gcagcctcca gggggagttt gacgttgcgc gagacgacgt
     4321 gcccggccaa ctcaagccgc agttgcaaac gtgcaacctg ttcggcgtgc gcttgctgtt
     4381 cctgtcggta aaggcgggcc acactccgct gggaaccgtg tacgaccgcc tgcgagccgc
     4441 cggcgatttg gcggcccgcc agggcgtcac gctcgcgctt gaaacccacc ctgacctggt
     4501 caccaacgcc caggttgcac gccagacgat ggaaggcgtc gaccacgccc acgtgcgcgt
     4561 gaacttcgat acggcgaacg tctatttcta caaccacggc gtcgatgccg tggaggaact
     4621 ccggcgcatg ctgccattcg tcgcgtcggt ccatctcaaa gataccgatg gcggcttccg
     4681 ctcctggcat tttcccgccc tgggccgcgg tatcgtaaac ttccgcggcg tgttcgaagt
     4741 tctggaccag gccggcttta cgggaccgtg cacgctggaa atagaaggca tcgaaggcga
     4801 agaaaagact gaacgcctcg tttgcgatcg catggccgag tcgatcggtt acctgcgcgg
     4861 gctaggtcgc ctgtaataaa tggcgcgcgc cgccgcgcct cgccgcagct cgtctcccgt
     4921 ggtggcggga acccgtcaga gcacaatccc agaaatgctt ccggttgacc gctggaccgc
     4981 ttacgccgat tcgtgcaaga tgaacccttt gccggccgac gggcaattca ggagcactcg
     5041 tgggtcacgt gacagaaccg agtttcgggt cggaacgcca gccagccaaa cgccccacag
     5101 agccaaccgg acgcataacg ccccgacggc ctgaagatgg cgcgccagcg gacggcgcaa
     5161 cgcgaatatc ggacgccctt ggtaccggaa ctgagcagag cccggcctcg ctgaacgccg
     5221 cgtcgccgcc tcccgtggcc gaccggcctg ccgcaactgc gccaccgccg gcccatccgc
     5281 cgacgccagc ccatacggcg gcggcgacag cggttccggc cagcgccccc gcccctgccc
     5341 cggccaagtt cgagccgccg gagtgggccc
//
LOCUS       DAOSYS010000245         5340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_12891_length_5340_cov_6.340776, whole genome shotgun sequence.
ACCESSION   DAOSYS010000245 DAOSYS010000000
VERSION     DAOSYS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12891_length_5340_cov_6.340776"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2759)
                     /locus_tag="PLU99_13050"
     CDS             complement(<1..2759)
                     /locus_tag="PLU99_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18019.1"
                     /translation="MKRGLCCVCAVLVCTALVFAGSPVRTGVDQKTGDAALTVTPAQP
                     QTTVSSLGNVSPSELWSNRVPAGAAAHVWTRPAADSREVVYSCETGGLPPVAEGEPAC
                     GPGYVDNYNGGCNSTPAVFQPAEIWMEGTSGFYEAEPGAWSRDTDWYEVLCSEPTRIT
                     YTVYSEFYVQVLLIDGTAGCEASTLLAYSVADPCREAVIDFCVPGLADDPLTPTVEIG
                     RYWLWVGPQFITDPSAMPCDSVRYCAHLETATCEYPEPLACPTTAIPNVGQLPYGQLE
                     SWQLFVSNDRFPDYDPANGFLRYEHFTGVGATSGGINKVYWWGVGATYNDQGNLVACD
                     LDPGTFTVTFWAHNKYTNRPFYVKEGWEWAYPPGAQCPLYSYTVTGADLTKEETGHVY
                     EVRGSDGSLSAAYPLYRWSCELPEAMTLFEGWISIQNSDLCPFWWAASPGPMFGTGGV
                     HVAENQGVDPVTRQDVDGDLSFCLGSTQSGPFTGACCDRSSDPAACRNTTADECVNPY
                     DEWHPSVTCAAISCTPEYWACCTGTGASGCHIGFEYSAAECTGTWYRWYVCSPADPGY
                     DADAWGGAGNVIVCPEQGAEAPITCDAAGMSFGRGLTGGYLYISWDRGTGDQRYVIDA
                     FNHPSSGAVRRIHWWGNSTTVWDGPDCDFANPAPFAIRFYHTSGSGPAYNAPHKVYDP
                     IYALYEETGYLWSGTEYGSIDRYSVTLPDPVTPGTGWQWISIASKTTNCDFIWAPGAT
                     GTSGFGQWFYNNGVCEATPGYRHRSFCLYTGVRTGACCNEITGTCLNGVQETTCASGG
                     GTFYPSQRCDQIPAPGCYAHTGACCDRSTFPASCTMTTSADCTGQWLGADVSCDMCCY
                     ACPADGMPEGEPECYDGYVDTYNNGCFTQPTWEFDDFVNIFDGQVVCGKTGTYRVSLP
                     DGSTSG"
     gene            complement(3270..5162)
                     /gene="mutL"
                     /locus_tag="PLU99_13055"
     CDS             complement(3270..5162)
                     /gene="mutL"
                     /locus_tag="PLU99_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOO18020.1"
                     /translation="MGEIRLLPANLVNRIAAGECVERPASVLKELVENSLDAGASRID
                     VAVLDGGRELIQVADDGMGMGVDDLRLSVAAHATSKIRSDEDLFSIHTMGFRGEALAS
                     IGAVSRLRIVSRTRDSEVAYALAVEAGAASEPIACAGPSGTCVEVRDLFYCVPARRKF
                     LRTNQTEMGHITEQFARIALAHPGVSFTLKNQNRPSYDLPATSDRRQRIADFYGPELA
                     EVLLPIRRAGDRILVEGYIAPPAQCRRSGKWEYVFVNGRFIRDRFISHAIKAAYRSLI
                     QPDEFPVAFLFITIQPDQVDVNVHPTKIEVRWRDSNYVHGQVLAALREKFLSTNLDRP
                     LRPPVAENDAYRERVREAMVDFFTHAKPKEPHSTPMWNSAPCRAIQPSPGDVHPAVRP
                     EQNDLKAPRSEGDTCTGYAQKTPAENFWASSANLCGELPPCGEADQNTAGLRAMQIHN
                     SYLVVEVEDGVLIIDQHALHERILYEQLRRRTADRPLESQRLLLPDVLRVPADRMEAL
                     EVHAELLQRLGLELTPAGPQDVAVHAFPQLLARANVAEFVRDLLDLLCEAGQRPNLEA
                     LVHDVLDMMACKAAVKAGDALTAQEISALLAQREVADRRSHCPHGRPTTLHLTLRELE
                     GQFKRR"
BASE COUNT         1033 a         1710 c         1584 g         1013 t
ORIGIN      
        1 ccactcgtcg agccgtctgg cagcgacacc cgatacgtgc cggtcttgcc gcagacgacc
       61 tggccatcga agatgttgac gaagtcgtcg aactcccagg tcggctgagt gaagcaaccg
      121 ttgttgtagg tatcaacgta cccgtcgtag cactccggct cgccttcggg cattccgtcc
      181 gcggggcaag cgtagcagca catatcgcaa ctgacgtccg cgcccagcca ctgtccagtg
      241 cagtcagcgc tggtcgtcat cgtgcagcta gccgggaagg tgctgcggtc gcagcaagcg
      301 ccggtatgcg catagcagcc cggagccgga atctggtcgc agcgctggga cggatagaat
      361 gtcccgccgc cgctcgcgca ggtcgtttcc tgaacgccgt tcaggcacgt gcccgtgatt
      421 tcgttgcagc aggcgccggt tcttacgccg gtgtagaggc agaagctgcg gtgccggtag
      481 ccgggcgtag cttcacacac accgttgtta tagaaccact ggccgaagcc cgacgtgccg
      541 gtcgccccgg gcgcccagat gaagtcgcag ttggtggtct tggacgcgat agatatccac
      601 tgccaaccgg tgccaggggt aaccggatcg ggcagtgtta cgctgtagcg gtcaattgaa
      661 ccgtattcgg tgcctgacca gaggtagccc gtttcttcat aaagtgcgta gatcgggtca
      721 tacactttgt gcggggcgtt gtaagccggg ccgctgccac tggtgtgata gaacctgatc
      781 gcgaagggcg cgggatttgc gaagtcgcag tccggaccgt cccacacggt ggtggagtta
      841 ccccaccagt ggatgcgcct gacggcgccg ctggagggat gattgaaggc gtcgatcacg
      901 taacgctggt caccggtgcc gcggtcccag gaaatgtaca ggtaaccacc tgtcagtccc
      961 cttccgaagc tcataccggc cgcatcgcaa gtaatcgggg cttccgcgcc ctgttccgga
     1021 cacacgatca cgttgccggc gccaccccag gcatccgcat cataccccgg gtcggccggg
     1081 gagcaaacgt accaacgata ccaggtgccc gtgcactctg cggcagagta ctcaaaacca
     1141 atgtggcagc cggacgctcc cgtacccgtg cagcaggccc aatactctgg cgtgcagcta
     1201 atggccgcgc aggtcaccga cggatgccat tcatcgtatg gattcacgca ctcatccgcg
     1261 gtcgtgttgc ggcaggccgc cggatcggaa ctgcgatcgc agcacgcgcc ggtgaacggc
     1321 cccgactggg tggaacccag gcagaaggac aggtcgccgt caacgtcctg acgcgttacc
     1381 ggatccacgc cctgattttc cgccacgtga acgccgcccg ttccgaacat cggaccgggt
     1441 gaggccgccc accagaacgg gcaaaggtca gagttctgga tgctgatcca gccctcgaac
     1501 agggtcattg cctcgggcag ttcgcagctc cacctgtaca gggggtatgc cgcgctaagg
     1561 ctgccgtcgg agccgcgcac ctcataaacg tggcccgtct cttccttggt caggtccgcg
     1621 ccggttaccg tgtaactata aagcgggcat tgcgcgcccg ggggatacgc ccactcccaa
     1681 ccttccttca cgtagaacgg gcggttggtg tacttgttgt gggcccagaa agtaaccgta
     1741 aacgtgccgg ggtccaggtc gcacgcgacc aggttgccct gatcattgta agtcgcgcca
     1801 acgccccacc agtagacctt gttgatccca ccggaggtgg cgccgacacc cgtgaagtgc
     1861 tcgtaacgca ggaagccgtt ggccggatca taatccggga agcgatcgtt gctcacgaaa
     1921 agttgccagg attcaagctg gccgtacggc agttgcccca cgttcggaat tgcggtggtc
     1981 gggcatgcca ggggctccgg atattcgcag gtcgccgttt ccaggtgggc gcagtagcgc
     2041 acgctgtcgc acggcatagc gctcgggtct gtaatgaact gcggtccgac ccacagccaa
     2101 taccggccga tttcgaccgt cggcgtcaag gggtcgtcgg caagtccggg aacgcagaaa
     2161 tcgatgacgg cctctctgca cggatcggcc accgagtacg ccaacaaggt gctggcttcg
     2221 cagcccgccg ttccgtcaat cagcaggact tgcacgtaga actcggaata taccgtatag
     2281 gtaatccgcg tcggctcaga gcataggacc tcgtaccagt cggtatcccg cgaccaagca
     2341 cccggctcgg cctcgtagaa gccgctcgtg ccctccatcc aaatctcggc cggttggaag
     2401 acggccggag tggagttgca gccaccgttg tagttgtcta cgtagcccgg gccgcaagcc
     2461 ggctcgcctt cggctacggg ggggagaccg cccgtttcgc acgagtacac gacctcccgg
     2521 ctatccgcag ccggacgcgt ccagacgtgg gcagccgcgc ccgccggcac gcggttggac
     2581 cacagttcgc tcggcgagac gttgcccagg gagcttaccg tggtttgcgg ttgggccggc
     2641 gtgacggtca atgccgcatc gccggtcttc tgatctacgc cggtgcgcac gggcgagccg
     2701 gcgaagacca gcgccgtaca aaccagcacg gcgcaaacac aacacaagcc tctcttcatc
     2761 gatgtctcct ttcggtgttc ctacccctac gccaaaccat cgccggcccc ttgccccgcg
     2821 cggaaccgtt taaacccagg ctcaccgcgc accctgggcg accggcaaac ataaacgcac
     2881 gctttaacac taatgtgcaa gccacacggc atgcgggcat agacactgcc tctcggccat
     2941 tgtgcgcagt ggacgggtga tgcttctctg ctcccatccc acccatgaac gcagcgcatt
     3001 cgagcctcat aataaatata cggcttcaga gtgccctatg caagagcatt aacacgcccg
     3061 tttcggaaac cgcgctctgc gccttgtccc aggcctctgg cctccaaggc cacactccgt
     3121 gcacgccaaa gttatgggac accttcaggc tcaaccattc gcaagtccat accgcacaga
     3181 cacttggtga aactcgcccg tgcgtgcccg tgctgctact cttctatcgg ctatccagcc
     3241 ctgacgagcc gaccttgttt taccggacct caccggcgct tgaactgccc ttccagctct
     3301 cgcaacgtca gatgcagcgt cgtcggacgc ccgtgcggac agtggctgcg gcggtcggcc
     3361 acttcgcgct gggccaataa tgcgctgatt tcttgggccg tgagcgcatc gcctgccttt
     3421 acggccgcct tgcaggccat catgtctaag acatcgtgta caagcgcttc caagtttggt
     3481 cgttgtcccg cttcgcacag cagatcaagc agatcgcgca caaattccgc cacgttcgcg
     3541 cgcgccagca attgtggaaa tgcatgcacg gccacgtctt gcgggccggc cggcgtcaac
     3601 tccagcccca gtcgctgcag caattctgca tgcacctcca gtgcttccat tcggtcggcc
     3661 ggaacgcgca agacgtccgg taacaataac cgttggcttt ccagaggccg atctgcagtg
     3721 cgccgccgca actgctcgta caagatccgc tcatgcagcg cgtgttgatc aattatcaga
     3781 actccatcct ccacctccac cacaaggtag gagttgtgga tttgcatcgc gcgcaggccg
     3841 gccgtgtttt gatcagcttc gccgcatggc ggcagctctc cgcacaggtt cgcagatgag
     3901 gcccagaaat tctcagcagg tgttttttgc gcgtaccccg tgcaggtgtc gccttcagaa
     3961 cgcggggcct tgagatcgtt ctgctcgggc cgcacagccg gatgaacatc acccggcgag
     4021 ggctggatcg cccggcaggg agcggagttc cacatcggtg ttgaatgtgg ctctttaggt
     4081 ttggcgtgcg tgaaaaagtc gaccattgcc tcacgcacac gctcacgata agcgtcattc
     4141 tcagctacgg gtggccggag cggccggtcg agattggtag acaggaattt ctcgcgcagc
     4201 gccgcaagca cttggccgtg tacgtagtta gaatcgcgcc agcgcacctc gattttggtg
     4261 ggatgcacgt ttacatcaac ctggtcgggc tgaatagtga tgaacagaaa cgccactggg
     4321 aactcgtccg gctgaattag actgcgatag gcggctttga tggcgtgaga gatgaatcgg
     4381 tctcgaataa agcgcccatt tacaaatacg tattcccact ttccggatcg tcggcattgt
     4441 gccggcggcg caatgtagcc ttcgaccaga attcggtcgc ccgcgcggcg gattggcaac
     4501 aggacttctg cgagttccgg accgtaaaaa tcagcaatac gctgtcgacg gtcggacgtt
     4561 gccggcaggt cgtatgacgg ccggttctga ttcttgagcg tgaacgagac tcccggatgc
     4621 gccaaggcaa tccgggcaaa ctgctcggta atgtgaccca tctcggtttg gttcgttcgc
     4681 aggaatttgc gacgcgccgg cacacaatag aacaggtcac gaacctcgac gcaggttccg
     4741 cttggtccgg cacatgcgat gggctcactg gcggcgcccg cctcaaccgc cagcgcgtac
     4801 gccacttcgc tgtcgcgcgt ccgcgagaca atccgcagcc gcgaaaccgc cccaatgctg
     4861 gccagcgctt cgccgcgaaa ccccatggtg tgaatgctga acaggtcctc gtcgctgcgt
     4921 atcttgctcg tggcatgggc cgccacgctc aaccgcaggt cgtccacgcc catgcccatc
     4981 ccgtcgtccg cgacctgaat cagttcccga ccgccatcca gcacggccac atcaatgcgg
     5041 ctggcgccgg cgtcgagtga attttccacc aattccttca gcaccgaagc gggtcgttca
     5101 acgcactcac ccgccgcgat gcggtttacc aagtttgccg gaagcagccg gatttcgccc
     5161 atcctcgacc tcgctgaccc gcccgcagta taacgggagc aatcctgacg gcgagcagga
     5221 gcaatcgtgc gagcagcacc aatcgtgacg gcgagcagcg gctgcggccc ttgttacgac
     5281 caacggtcgg gtgaaactat gaagcggagg tcgcgccgcg gtagtttagg ttcctggaga
//
LOCUS       DAOSYS010000246         5289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13041_length_5289_cov_5.759648, whole genome shotgun sequence.
ACCESSION   DAOSYS010000246 DAOSYS010000000
VERSION     DAOSYS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13041_length_5289_cov_5.759648"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLU99_13060"
     CDS             complement(<1..1232)
                     /locus_tag="PLU99_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18021.1"
                     /translation="MARNAILLLSTLALFGALFLGYLVVVQDPVLDAGGDSPEILPVD
                     RGRAGEALRLPGVEVPSGGGVSFTIYHRETGRPVQRFECEEWTPVADAKDEVYVRRPR
                     LTMWLPSGMIATMSADEGQLQVEDLEKVQGRPRAGWLKGNVELLLDRSRERQRTPLSE
                     RPQDEILAMLDQIDFDFESGELRTDGPIKIVGHDFELAGVGLHLVWNQADNVIQSLTL
                     REGERLVLYGGGGLLRNEDENAAAAEAATAEEAMAAATSVKQVAAPKQRRPKKAQKAY
                     LCVLPDGVTAYEFKGETTGGGLVADEVRMLFEVGGGSHDLTQTPAEQPVEKEAAHPES
                     APASAPASAPTTAPAERTGVIVHWGGELRVEPVPADSGEKPRRHLEALGTEVKLWRGD
                     NKLVCSRLEYHDETKQLWL"
     gene            complement(1270..1773)
                     /locus_tag="PLU99_13065"
     CDS             complement(1270..1773)
                     /locus_tag="PLU99_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOO18022.1"
                     /translation="MPEIRCVCLDVDGVLTDGRIFVDSEGRSARAFYVQDGFAIRWFQ
                     KLGGVAVILSGKSCAAVEARARDLGIEHVIQGSSDKLADLRTLAARLALQLSEIAFIG
                     DDLPDLPPMRACGFPVAVANAVPEVKAAAKLVTQRAGGQGAVREAIEHVLRRDGRWQE
                     VLKYYGV"
     gene            1993..2868
                     /locus_tag="PLU99_13070"
     CDS             1993..2868
                     /locus_tag="PLU99_13070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO18023.1"
                     /translation="MNPTAEHPARFDLGGCTVTLINGGRLRIDGGAMFGIIPKALWSR
                     TNPADEQNRIEMACNCLLVEWPGEAARRVIIETGHGPKLSPKEQKIYAADPQQWLLPS
                     LQALGVDPLTITDVIVSHLHFDHAGGLTYETEGRLLPTFPNARVHVQRQEYEDARAGF
                     GITNISYPRQNLDPIDQAAAWNLLDGEVEIVPGIRALLAPGHTRGHQAVLVAGRERSL
                     VFGGDTIPTAGHLGAAYNMSYDLSPLENRASKYKLLQMLAQRGGVLLIDHEPRTPAVS
                     VRAAGDWFELAPGAE"
     gene            2944..3765
                     /locus_tag="PLU99_13075"
     CDS             2944..3765
                     /locus_tag="PLU99_13075"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HOO18024.1"
                     /translation="MTSTGLRNLSILAVLLTAVTAIGQQNAAPPAEGVAAQPATTQAA
                     TTQPAIEAKADELLQKMSGQLAKLSAFRLEAEILFDQLSPDGQKIQYSNRFTVAQKRP
                     DQLRGELTGDMPGRRFWYDGRELVTLDLERNVYSVVPVTGTVDAMLDHTMERYGWTIP
                     LSDLLLSNPHEALTGEALSGRYLGLHRVGEFKCHHLAFTQDDLDWQIWIDDDEQALPR
                     KLVIDYRSLPGSPQFSAVIVKWDTKPELKADDFKFEPPPGATKIDMLPVEPEQQD"
     gene            3794..4699
                     /locus_tag="PLU99_13080"
     CDS             3794..4699
                     /locus_tag="PLU99_13080"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HOO18025.1"
                     /translation="MTIRTSFIGAIALGIAALLMADVAAAADAKPAQKSAQSGQPTGS
                     VRHSRKRTARPPAAGTAAQQRQTRGTPQAATPRSGDDRARRGSSTPVTRADDRDQRRD
                     SERGTRGRSVERDVRRSDDRHRAPLLHEYRGVRRPNVGHREIYDGHGSVRAYGKRPAR
                     GSVFLRPPERCEVVHHGPRRYFVYDDVYYVERPVLGEVRYVVVPPPVGVRVRRLPETA
                     VAIQIGGFSFYYCDDTYYRVEYVDHGPEYVVTETPLGASILQLPEEHEPVVVQEVTYY
                     RVGKVFYRPYFREGRVVYVHVQPPR"
     gene            complement(4826..>5289)
                     /locus_tag="PLU99_13085"
     CDS             complement(4826..>5289)
                     /locus_tag="PLU99_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO18026.1"
                     /translation="ITRLARAYNTEVPHSGKILTGGVDANALQKPKRFFGAARNIEEG
                     GSLTIIGTALVDTGSKMDEVIFEEFKGTGNSELHLDRRLVDKRIWPAINIAASGTRKE
                     ELLLDPKELEKVYLLRRVLSDMNPVESVELLRGRLEKTDSNAVFLMTMNLA"
BASE COUNT          993 a         1827 c         1585 g          884 t
ORIGIN      
        1 agccagagct gcttggtttc atcgtggtat tccaggcgac tgcaaaccag tttgttgtcc
       61 cccctccaca acttgacctc ggtcccaagc gcttcgaggt ggcgccgcgg cttctctccc
      121 gagtcagccg gcacgggttc aacccgcaac tccccacccc aatgcacgat cacgccggtg
      181 cgctccgccg gcgcagtcgt tggcgcactt gccggcgcac tcgcgggcgc tgactccggg
      241 tgtgcagcct ctttttcgac cggctgctcc gcgggcgtct gcgtcaggtc atggctgccg
      301 ccgccgactt cgaaaagcat tcgcacttcg tcagcgacta gccctccgcc ggtggtttcg
      361 cctttgaact cgtaggcggt cacgccgtca ggcaggacgc ataagtaggc cttctgcgct
      421 tttttgggcc gccgttgctt gggcgcagcg acctgcttga ccgatgttgc ggcagccatc
      481 gcttcttctg ccgtcgcagc ttctgccgcc gcggcattct catcctcgtt gcgcagcagc
      541 ccgccgccgc cgtaaagcac caggcgctca ccctcccgca gcgtcaggct ctgaatgacg
      601 ttgtcggctt gattccagac caagtgcagc ccaacgccgg ccaactcgaa atcgtggccg
      661 acgattttga tcgggccgtc cgtgcgcagc tcgcccgact cgaaatcaaa gtcgatttga
      721 tcgagcatgg cgagaatctc atcctgcggc cgctccgaaa gcggcgtgcg ctgccgttca
      781 cgcgaacggt ccagcagcag ttcgacgttc cccttcagcc agccggcgcg cggccgccct
      841 tgcaccttct cgaggtcttc gacttgcaac tgcccctcat cggcggacat cgtggcgatc
      901 atgccactcg gcagccacat ggtcagcctt ggccgcctga cgtaaacctc gtccttcgcg
      961 tccgcgaccg gcgtccactc ctcgcattca aagcgctgca cgggccgccc ggtctcgcgg
     1021 tggtaaatgg tgaacgaaac gccgccgccc gagggcacct ccacgccggg caggcgcaag
     1081 gcctcacccg cgcggccgcg gtccacgggc aaaatttccg gggaatcgcc gcccgcgtcg
     1141 aggaccgggt cctgcaccac gaccagatac cccaggaaaa gcgcgccgaa cagcgccagc
     1201 gtcgaaagca gcaaaatggc attgcgggcc atgctcgggc ttccttaccg cacctctagc
     1261 cagcccccgt caaacgccgt agtacttcag aacttcctgc cagcggccgt cgcgccggag
     1321 cacgtgctcg atggcctcgc gcacggcgcc ttgcccaccg gcccgctggg tgaccagttt
     1381 cgccgccgct ttcacctcgg gcacggcgtt ggcgacggcg accggaaagc cgcaggcgcg
     1441 catgggcggc aaatcgggca gatcatcgcc gatgaaggcg atttcggaca attgcagggc
     1501 cagccgggcg gccagtgtcc gcagatcggc cagcttgtcc gaactgccct gaatcacgtg
     1561 ctctatgccg agatcgcgcg cccgggcctc gaccgccgcg cagctttttc ccgagagaat
     1621 taccgccacg ccaccgagct tctgaaacca gcggatcgcg aagccgtcct ggacgtaaaa
     1681 ggcccgcgcc gaccgccctt cgctgtccac gaagatgcgc ccatcggtca acacgccgtc
     1741 cacatccaga cacacgcagc gaatctcagg cataatatcg cctcagctaa tcgccctgcg
     1801 cgagcgcgag aatccgtgtc agaagcgcca gcgagggcca acgctcaccg gggccaacgc
     1861 tcgccgaagc ctcgccagga tggggagcgc tgccaacgag ccacggcgat tattagttcc
     1921 cagccgcgcc gcaatgcgga tactatcaca aaaaaccggc tggcacgatg aacaccgcac
     1981 gatgagtacc gcatgaatcc gactgcagaa catccggcac gtttcgacct cggcggctgc
     2041 accgtcacgc tgatcaacgg cggacgcctg agaatcgacg gcggcgccat gttcggaatc
     2101 atccccaagg ccctctggtc gcgcactaac ccggccgacg agcaaaaccg gatcgagatg
     2161 gcctgcaatt gcctgctagt ggagtggccg ggcgaagccg cgcggcgggt aataatcgaa
     2221 accggccacg gcccaaagct cagtcccaaa gagcaaaaaa tctacgccgc tgatccgcag
     2281 caatggctgc tgccgtcgct tcaagcgcta ggtgttgatc ctctcactat cacggatgtc
     2341 atcgtatcgc acctgcactt tgatcacgcc ggcgggctga cgtacgaaac cgaaggccgg
     2401 ctgctgccga cctttcccaa tgcgcgcgtg cacgtgcaac ggcaggagta cgaagacgcc
     2461 cgcgccggtt tcggcatcac gaacatcagc tacccgcggc agaacctgga ccccatcgac
     2521 caggccgccg cctggaatct gctggacggc gaagtcgaga tcgtcccggg catacgcgcc
     2581 ctgctcgccc ccggtcacac gcgcggacat caggccgtcc tggtcgccgg ccgcgagcgc
     2641 agcctggtct tcggcggcga caccatcccg acggccgggc acctgggcgc ggcctataac
     2701 atgagctacg acctctcccc gctcgaaaac cgcgcatcga aatacaaact cttacaaatg
     2761 ctggcccagc gcgggggcgt gctgctcatc gatcacgagc cacgcacgcc cgccgtctcc
     2821 gtgcgcgccg caggcgattg gttcgagctt gcccccgggg ctgaataaag ccccacctac
     2881 aggcgtccat cgggtacaat cccgccctcg gccgcatgcc gcggctgaga atgggagatg
     2941 aatatgacct cgaccggttt gcgaaatctg tcgatccttg ccgtactgct caccgctgtc
     3001 accgccatcg gccagcagaa cgccgctcca ccggccgaag gcgttgcggc gcagccggcc
     3061 acgactcagg ccgcaacgac ccaaccagcc atcgaggcga aagcggatga actgctgcag
     3121 aagatgagcg gccagctcgc caagctgagc gcctttcggt tagaagcgga gattctcttc
     3181 gatcagctct cgcccgacgg acagaagatt caatattcaa accgctttac cgtggcccag
     3241 aaacgccccg atcagctaag aggcgaactg acgggcgaca tgcccggccg gcgcttctgg
     3301 tacgacggcc gcgaactcgt gacgttggat ttggaacgca acgtatactc cgtcgtaccc
     3361 gtaacgggca cggtagacgc catgctggat cacacgatgg agcgttacgg gtggacgatt
     3421 ccgctatcgg acttgctgtt gagcaatccg cacgaagccc tgaccggtga ggcattgagc
     3481 gggcgttatc tcgggctgca tcgcgtgggc gaattcaagt gccatcacct ggcgttcacg
     3541 caggacgacc tggactggca aatctggatc gatgacgacg aacaggccct gccgcgcaag
     3601 ctggtaatcg actaccgcag cctgcccggc tccccgcagt tctctgccgt tatcgtcaaa
     3661 tgggatacaa agccggaact gaaggctgac gattttaagt tcgagccgcc gccgggcgct
     3721 accaaaatcg acatgctgcc cgttgaaccg gagcagcagg actagcgccc caaaactcta
     3781 aggaggatga gccatgacca tccgcacaag cttcatcggc gcgatcgcac tggggatagc
     3841 ggctttgcta atggccgacg tagcggccgc ggcagacgcg aagccggctc agaagtcggc
     3901 tcaatcgggc cagcccacgg gctcggtccg ccacagcagg aagcgaacgg ctcggccgcc
     3961 cgccgccggc accgctgctc agcagcgcca gacccgcggc acgccgcaag ccgccacgcc
     4021 ccgcagcggt gatgaccgcg cgcggcgcgg ttcgagcacg cccgtcacgc gggccgacga
     4081 ccgcgatcag cggcgtgact ctgaacgcgg cactcgcggg cgcagcgttg agcgcgacgt
     4141 tcgccgctca gacgaccgcc accgcgcccc ccttctgcat gaataccgcg gcgttcgccg
     4201 gccaaacgtc ggccaccgcg aaatctacga cgggcatggt tccgtccgcg cgtacggcaa
     4261 gcgcccggcc cgcggcagcg tgttcctgcg cccgccggag cgctgcgaag tcgttcatca
     4321 tgggccgcgc cgttacttcg tgtacgacga cgtttactac gtagagcggc cggtgctggg
     4381 cgaggttcgc tatgtagtgg tgccgccgcc ggtgggcgtg cgcgtgcggc gtctcccgga
     4441 aacggcggtc gcgatccaga tcggcggatt cagcttctac tattgcgatg acacgtacta
     4501 cagggtcgag tatgtcgacc acgggccgga atacgtggtg actgaaacgc cgctgggcgc
     4561 ctccatcctg cagcttccgg aggaacatga gccggtcgtg gtgcaggagg tgacgtacta
     4621 ccgcgtgggc aaggttttct accggccgta cttccgcgaa ggccgcgtcg tttacgtcca
     4681 cgtacagccg ccacggtaaa gagcgggccg gggggcgaac tcaaaccggc ccgtacgccc
     4741 ataacccaag cgcaagagaa ggcctcgcgc tccaaagcct ggatcgcgag gcctcgttca
     4801 ttcagcattc cggggcggaa gaatcctacg ccaaattcat cgtcatcagg aacaccgcgt
     4861 tcgaatccgt cttttccagc cgcccgcgca gcagctcgac cgactcgacc gggttcatgt
     4921 cgctcagcac gcggcggagc aggtagacct tctccagctc cttcggatcg agcaacagct
     4981 cttccttgcg cgtgcccgaa gcggcgatgt tgatggccgg ccagatgcgt ttgtcgacca
     5041 gacgccgatc caggtgcaac tcgctgttac cggtgccttt gaactcctcg aaaatgacct
     5101 cgtccatctt cgagccggta tcgaccaggg ccgtgccgat gatcgtcagg ctgccgccct
     5161 cttcgatgtt gcgggccgcg ccgaagaagc gcttcggctt ctgcagggcg ttcgcgtcaa
     5221 caccaccggt cagaatcttg cccgagtgcg gcacttcggt gttgtaggcg cgggccagac
     5281 gcgtgatcg
//
LOCUS       DAOSYS010000247         5270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13105_length_5270_cov_4.200575, whole genome shotgun sequence.
ACCESSION   DAOSYS010000247 DAOSYS010000000
VERSION     DAOSYS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13105_length_5270_cov_4.200575"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..2029
                     /locus_tag="PLU99_13090"
     CDS             62..2029
                     /locus_tag="PLU99_13090"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOO18027.1"
                     /translation="MRRAILSVVVAFVAFQIQTSPGMADEREPDELDVPYEPTHPQVV
                     EAMLAVAGVCERDIVYDLGCGDGRIVVQAAKTRGARGLGVDLDPQRVREARENAAHHA
                     VERLVEFREADLMQTDLHAATVVTLYLLPSVNLMLRPKLLSELQPGTRIVSHAFDMAE
                     WEPDKTLKQPRARAERVYFWVVPAAAGGFWQWSGVGPEPGTFVVRLNQCFQRLSGELT
                     GEAGAPVSLNEAVLSGKQIALTVSIQKGPEPVEVILHGVIEGETIKGIQQWIDGASVQ
                     ERPWVAQRTAVDVSGAWQMADCPELPNGTLRIERNAGKAKATFTPAGGVEQTLPEFYA
                     WGACLRFEVPRERGSALLCNGFVHGDVVEGTVSGGGLSRVGQWSAKRVVAGAPSTQSA
                     VPAAGRQWTPQLGPPEVGDEYRNPKDGSVLVWIPGGEFNMGRDDGPDDERPAHTVRLD
                     GFWMGKFEVTNRQFAQFLKDTGGSTPMFWDDPNLSGPEQPVVGVTYSEAQAYCDWAGL
                     RLPTEAEWEYAAGGGRGLAYPTATGEIGPDLANLRGVSGRDRWEFTAPVGQFPPNPWG
                     LCDLAGNAWEWTSSRCSSYPYRLTDGREDDVGGLRVMRGGCWAFPADYAATTRRHRFS
                     PYLRYDYPGIRVARSALGAEPQAVRASVLRP"
     gene            2176..4944
                     /locus_tag="PLU99_13095"
     CDS             2176..4944
                     /locus_tag="PLU99_13095"
                     /inference="COORDINATES: protein motif:HMM:NF027637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HOO18028.1"
                     /translation="MNKLRRSILVCLIGAMGIFSLALLPGLAAAQSQEPEHQQTPSDQ
                     IAQPRVPRSGGSGDQADDEKPRFPDAKEVTKDMKASEGLFTLYRFDPNDKKRDPEKLL
                     AKIPQNLLGEDLLLAMSISRGRMAGFMWGDALVRWEIAGKQLRLMTPELRYVRKPGQP
                     VSEAVERTYNDSFMAAVPIVGMTQQGDPIIDLEPLLKSDLAGVTSVASGRIQPELSTW
                     RTVKTFPDNVLIDVDMAVGAKRGGQQVGITYGFRRLPKLGSYSPRRADPRVGYFLTAK
                     MDWSKKYNERENFDRYIHRWKLEKQDPTLELSPPKEPIVFIIEKTVPIQWRRWVREGI
                     EDWNKAFEKIGFTGAVVVQQQTDDNEYKDYDPEDARYNFIRWGVTGRAFAMGPSRVDP
                     RTGQILDADILFDDSFVRAWMYNFDLYGPAAMAQLKGPGFEEFARAHPDLVPTFLQAP
                     STGEDPDQEFWAALHETLEEQGRCTCSYAWGMQQQLALGYNAMIATGTGAKKLPERFI
                     GEAIKEIVTHEVGHTLGLRHNFKGSSWLSMEEIQRRRQETDEPTTASIMDYNPLLFFA
                     EDEPDKCRHFITPTIGPYDEWAIAYGYAVPQDKSETEMLKEIAQRGTEPALQYATDED
                     TVWVYSPDPLVNRYDLSSNPIDYSRARMALTGKLLKNITEWAVQDGEPMYYLRRAFDV
                     IWLERFRNMSFVARTVGGQYFNRDNRGDPNARPPFVLVEPAKQREALDFLAETVFADE
                     FYMVDPEVLNNLAPSRWGHWGSDAPAQLDYPMHERIRYMQVQILLDLLAPPVLQRVYE
                     AELKSMAADKFTAAELLTTLRDKVWKQLDSKPGGTYTNAEPFISSISRNLQQEHLSIM
                     LNMVASRPGSTVSKDLHAMLTLTVRELSEKIGHTLDGHKLDFASKAHLTECKSRIDRI
                     LSAPLRAE"
BASE COUNT          986 a         1569 c         1770 g          945 t
ORIGIN      
        1 gaggagccga aagaaccgtc ggtcttgggc ccgaacaccg gcccgcacca ggagagatga
       61 aatgaggcgc gcaattctgt ctgttgtcgt cgcgttcgtt gctttccaga ttcagaccag
      121 tcccggcatg gcagatgaac gcgagccgga cgaactggac gtgccctatg aacctaccca
      181 cccgcaggtg gtcgaagcca tgctggcggt ggccggcgtg tgcgagcggg acatcgttta
      241 cgacctgggc tgcggggatg ggcggatcgt tgtgcaggcg gccaagaccc gcggggcgcg
      301 cggcttgggc gtcgatctgg acccgcagcg cgtacgcgaa gcccgtgaga acgccgcgca
      361 tcacgctgtg gagcggctgg tcgaattccg cgaggcggat ctgatgcaaa cggacctcca
      421 cgcggcaacc gtcgtcacgc tctacctgct cccgagcgta aacctgatgc tgcggcctaa
      481 gctgttgtct gagctccagc ccggcacgcg catcgtctca cacgccttcg acatggcgga
      541 atgggaacca gacaagacgc tcaagcagcc gcgagctcgc gctgaaaggg tgtacttctg
      601 ggtcgttcct gcggcggccg ggggattctg gcagtggtcg ggcgtgggac cggagccggg
      661 aacgtttgtg gtccgactga accagtgttt ccagcggctg agcggcgaac tgaccggcga
      721 agcgggcgcg ccagtgagcc tgaatgaagc ggtactgtcg ggcaagcaaa tcgcccttac
      781 ggtcagcatt cagaagggcc ctgaaccggt cgaggtgatt cttcacggcg tgattgaagg
      841 tgaaacgatc aagggcatcc aacagtggat tgatggagcg agcgttcagg agcggccgtg
      901 ggttgctcaa cgcaccgccg tcgacgtgtc gggcgcgtgg caaatggcgg actgccccga
      961 gttgcccaat gggacgctca gaatcgagcg gaacgcgggc aaagcaaagg ccacgttcac
     1021 acctgcgggc ggcgtggagc aaacgctgcc ggagttctac gcctgggggg cttgtctgcg
     1081 ctttgaagtg ccgagagagc gcggcagtgc tttgctctgc aatggcttcg ttcacggcga
     1141 cgtggtcgaa gggacggttt ccggcggtgg cctctcgcgc gtggggcagt ggtcggccaa
     1201 acgcgtcgtg gccggcgctc cgtccacgca atccgctgtt cctgccgcgg gccggcagtg
     1261 gacgccgcaa ctcggaccgc cggaagtggg agacgaatac cgcaacccga aggatggctc
     1321 agtgctggtc tggattccgg gcggcgaatt caatatgggc cgcgatgacg gtcctgacga
     1381 tgagcggccg gcgcacacgg tgcgtttgga cggcttctgg atgggcaagt ttgaagtcac
     1441 gaaccggcag ttcgcccagt ttctgaagga cactggcggc agcacgccga tgttctggga
     1501 tgatccgaac ctcagtggac ccgagcagcc ggtggtgggc gtcacgtact ccgaggctca
     1561 ggcgtattgc gactgggcgg ggctgcgctt gccgaccgaa gcagagtggg agtacgcggc
     1621 cggcggcggg cgagggctcg cgtacccgac tgcgacggga gagatcgggc cggacctggc
     1681 gaatttgcgg ggcgtttccg ggcgggaccg ctgggaattc accgcgcccg tggggcagtt
     1741 cccgccgaat ccgtggggcc tgtgcgatct ggcggggaat gcgtgggagt ggaccagcag
     1801 ccggtgcagt tcttacccgt atcgtttgac cgatgggcgg gaagatgacg tgggcgggct
     1861 gcgcgtgatg cgcggcggct gctgggcctt tccggcggac tacgcggcca cgacgcgccg
     1921 ccaccgtttt tcgccgtact tgcgctacga ctacccgggg attcgcgtag cgcgctcggc
     1981 gctgggggct gagccgcagg ccgttcgggc gagcgtgctt cggccctgaa ctgtctgagc
     2041 ggcatgccgt acattcatct tgaggccttc attctatttc gggcgctcgc attctcgata
     2101 cggcgcgtta ggatatcggg ccgtgagccg gtcgggctgt ggcgggccaa gattcgcagt
     2161 cagcggagag cagttatgaa taagcttcgg aggtcgattc tcgtttgtct gataggcgcc
     2221 atgggcattt tcagtctcgc gcttttgccc ggccttgcgg cggcccagtc gcaggagcca
     2281 gaacatcagc agacgcccag tgatcagatc gcccagccgc gcgttccccg ctcaggcgga
     2341 tccggcgatc aggccgacga cgaaaaaccg cgctttcccg acgcgaaaga agtcacgaaa
     2401 gacatgaagg cgtcggaggg gctgttcacc ctctatcgct tcgaccccaa cgataagaag
     2461 cgcgacccgg agaagttgct cgccaaaatc ccgcagaacc tcctgggcga ggacctgctc
     2521 ttggctatga gcatctcacg cggccgcatg gcaggcttca tgtggggcga cgccctggtg
     2581 cgctgggaga tcgccggcaa gcagcttcga ctgatgacac ccgagctgcg ttacgtccgc
     2641 aagccgggtc agccggtaag cgaggcggtc gagcgcactt ataacgacag cttcatggcc
     2701 gcggttccga tcgtcggaat gacgcagcag ggcgacccga tcatcgacct cgagccgctg
     2761 cttaagtcgg acctggcggg cgtgacctcg gtcgccagcg gccgcattca gcccgagctg
     2821 agcacctggc ggacggtcaa gaccttcccg gataacgtgc taattgacgt ggacatggcc
     2881 gtgggcgcga agcgcggcgg gcagcaggtc ggcataacgt atggcttccg gcggctgccg
     2941 aagcttggct cgtacagccc acggcgtgcc gatccgcgcg tggggtactt cctgacggcg
     3001 aagatggatt ggtcgaagaa gtacaacgag cgtgagaact tcgaccgtta catccaccgc
     3061 tggaagctgg agaagcagga cccgacgctg gagctgtcgc cgcctaagga gccgatcgtt
     3121 ttcatcatcg aaaagacggt gccgatccaa tggcggcggt gggtgcgcga aggcatcgag
     3181 gactggaaca aggcgtttga gaagatcggt ttcacgggcg ccgtcgtggt ccagcagcag
     3241 acggacgaca acgagtataa ggactacgac cccgaggacg cccgctacaa cttcatccgc
     3301 tggggcgtta ccgggcgggc tttcgcgatg gggccttcgc gcgtcgaccc gcgcaccggc
     3361 caaattctcg acgccgacat cctgttcgac gactcgttcg tccgcgcctg gatgtacaac
     3421 ttcgacttgt acggtccggc tgctatggca cagctcaagg gtcccggctt cgaggagttc
     3481 gcgcgggccc atcctgacct cgtcccgacg ttccttcagg cccccagtac gggcgaagac
     3541 cccgaccagg agttctgggc cgcactgcat gagacgctgg aggagcaggg ccgctgcacg
     3601 tgcagctatg cctggggcat gcaacagcag ctcgcgctgg ggtacaacgc catgatcgca
     3661 acgggcaccg gcgcgaagaa gctgccggag cgcttcatcg gcgaggccat caaggagatc
     3721 gtaacgcacg aggtcggcca cacgctgggt ctgcgccaca atttcaaggg ctcaagctgg
     3781 ctcagcatgg aagagattca gcggcgccgg caggaaacgg atgagccgac cacggcctcg
     3841 atcatggact acaaccccct gttgttcttt gccgaggacg agccggataa gtgccggcac
     3901 ttcataacgc cgaccatcgg cccctacgac gagtgggcga tcgcctacgg ctacgcggtg
     3961 cctcaggaca agtctgaaac ggaaatgctc aaggagattg cccagcgcgg tacggagccg
     4021 gctctgcagt atgcgaccga cgaggatacg gtgtgggtct actcgcctga cccgctggtc
     4081 aaccgctacg acctgtccag caatccgatc gactactcgc gggcgcgcat ggcgctgacc
     4141 gggaagctgc tcaagaacat cactgagtgg gccgtgcagg acggcgaacc gatgtactac
     4201 ctgcggcggg cgttcgacgt gatttggctc gaacgcttcc gcaacatgag cttcgtcgcc
     4261 cgcacggtcg gcgggcagta cttcaaccgc gacaaccgcg gcgaccccaa cgcccgcccg
     4321 cccttcgtgc tggtcgagcc tgccaagcag cgcgaggctc tggacttcct ggccgagacc
     4381 gtcttcgccg acgagttcta catggttgat cccgaggtgc tgaataacct ggcgccgtcg
     4441 cgctgggggc actggggctc cgacgctccg gcgcagctcg attacccgat gcatgagcgc
     4501 atccggtata tgcaggtgca aattctcctg gatttgctcg ccccgccggt gttgcagcgc
     4561 gtctacgaag ccgagctgaa gtccatggcc gcggacaagt ttacggcggc cgagctgctg
     4621 acgacgcttc gcgacaaagt ctggaagcaa ctggactcca agccgggcgg cacctatacg
     4681 aatgccgagc cgttcattag ctcaatctcg cggaacctcc agcaggaaca tctgtccata
     4741 atgctcaata tggtggcgtc ccgcccgggt tcgaccgtgt cgaaagacct gcacgcaatg
     4801 ctcacgctga cggtgcggga gttgagcgag aagatcgggc acacgctgga cggccacaag
     4861 ttggattttg cctcgaaggc gcatctgact gaatgtaagt ctcgcattga caggatctta
     4921 agtgcaccgc tgagggcgga ataagcgctg tcggccactt gtgcctcaag agatttgccg
     4981 cccggtccta tatccatagg ccgggcggtt ttcattaggt gtatgtacgc cattgatggt
     5041 ccgaaatgcc gcggcttcag cgcttgactt tgttcagtat caagaggcgt caatcgctgc
     5101 taactcttta ccctgcctag aattccgctt gattgacgag gcggcttgcc gtatcatgac
     5161 cgggtgtccc gagtgccgcg ccttgcgcaa gtaaagccgg tcggacaagt ttgtcttgga
     5221 gatggccgcc cctcggcggt tcggacaggg gaacgttgat gaagacgatc
//
LOCUS       DAOSYS010000248         5247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13186_length_5247_cov_4.445878, whole genome shotgun sequence.
ACCESSION   DAOSYS010000248 DAOSYS010000000
VERSION     DAOSYS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13186_length_5247_cov_4.445878"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLU99_13100"
     CDS             <1..400
                     /locus_tag="PLU99_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18029.1"
                     /translation="AVKARNLSGMETPLGPGATLSTEGGGDGACCDPSGACSVLAAAA
                     CAGTYMGDGTACTPDPCCTGQLKGDANCDGVVNVFDIDPFVVALTSLDVWQGTYQGAG
                     CGFLCVNDCNGDGEVNVFDIDPFVQYLTGG"
     gene            701..1453
                     /locus_tag="PLU99_13105"
     CDS             701..1453
                     /locus_tag="PLU99_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO18030.1"
                     /translation="MIEVQELTKVFPNPDGSEKTAVDNISFRVLAHEIYGLLGPNGAG
                     KTTTLRMVSGLLRPTSGRVLINGKDVTNHEAAVKRQIGYLTANTGLYQRLTPLEILEY
                     FAVLYDIPRPQAKQRIAELVDWLGMHDFIKLRCGALSTGQKQRTNIARALIADPPVLI
                     LDEPTLGLDVLSNRLILQFIQSQADAGKAVLLSTHALDEIEKMCGRIGLIHDGRLIAE
                     NDLEGLKRQTGQSRLSDAFLHLVHANEPLLAD"
     gene            1431..3707
                     /locus_tag="PLU99_13110"
     CDS             1431..3707
                     /locus_tag="PLU99_13110"
                     /inference="COORDINATES: protein motif:HMM:NF041647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOO18031.1"
                     /translation="MNRFSRISTIWRKEFADTLRDRRTLVAMVLVPMVLYPALMLGSL
                     QAFELQVSKLKQQEYSIAVASDEVRHWLQTRILDPDLARFAAAAGLPAEELPAAVEAA
                     EKEADAQSGDSGRGRLEKEEAAVHRRPPPYRLEVVSDVRLAVATGMTNVGLLVSGELL
                     PTPDSAGACQITLVMDKAEIRSQFAASALEGVFKRLSERVVARRLQDLHLTRDYIEPY
                     QITQLSVATPEKLGGSIIGQIVALILVVMTITGAIYPAIDLTAGERERGTLETLMAAP
                     VPKVDLIAGKFVVVASIGMLSAVLNLLSIGGTVYLGGLGDVLTKEGGITIPLRTLPLV
                     LVTLIPLAVMFSAVLLAVCSFARSFKEAQNYIMPVMVVALIPAVVGLLPGTELEGPLL
                     IMPVTNIVILTRELFMGEINRLGVLWVTLSTCIYALAAVGVAAKLFGQEAVLFADSAS
                     IRTLLKRKFFKPSNWPSASQALLLMAVVYLLNFFIQQSLFQAGLTPGSPEHLAGFALT
                     LVLLLAALPIGVALYTRVRITTAFSFSPPRWRGLAAGLCIGLSTWVLALVWMSLQERI
                     LPLPPDFKEAAAPYEQMLQRLSVATVLFFFAILPAFCEELFFRGYALSGLRHVMGKTG
                     AVLLVAFAFGMTHMSIQRLPLTAAVGALLGLLVIRWGSIWPAVLAHVLHNSIILLITR
                     EDGLLPLLKRAGFVDPVTEDVFPPLPWIVGAAVLLLAGLALCVKRSRPVGQLADGLGQ
                     SGGDLLGVAGQQGPAVKD"
     gene            complement(3612..5237)
                     /gene="selA"
                     /locus_tag="PLU99_13115"
     CDS             complement(3612..5237)
                     /gene="selA"
                     /locus_tag="PLU99_13115"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOO18032.1"
                     /translation="MRDMSTPDTHENDASAPTGNEASSPQSTDAGPPGPMQPPDAGPL
                     QAAMRSLPAVDQLLKAPAVANLLAEFPREELLHAIRAVLNQRREALRGGEKVDVDTPS
                     LALAVREKLHERARPHLRRVINATGIVLHTGLGRAPLAQEAIDAIADIAAGYCNLELD
                     LPTGRRGDRHEHARELLAELTGAEDALVVNNNAAGTFLTLAALTAGREVIVSRGQLVE
                     IGGSYRMPDIMAAAGCRMIEVGTTNRTHLYDYERAISEASVALLRVHTSNYRTQGFVE
                     SVSLDDLVKLARSRGLLVIDDLGSGLIDRELPWPDEGGGAPGGPVIAQAPSGWAATTQ
                     APAGRAPSQWDEPAVRDSVASGADVVLFSGDKLLGGPQAGVIIGRADLIKRIRQSPLA
                     RAVRPDKMTLAGLEATLRLYRDPEALARRLPVFRMLAASPAELENSAVELADAIRQVL
                     PEAQVTQQPDTSEAGGGSLPMISFPTWIVIVRHPALPSMRLAAAVRQRETPVICRVQE
                     EALVFDCRTLLAGDAEQIAAALAEAVRELADGS"
BASE COUNT          846 a         1773 c         1698 g          930 t
ORIGIN      
        1 cgcggtgaag gcgcggaact tgagcgggat ggagactccg ctggggccgg gcgcgacgct
       61 cagcactgag ggcggcggcg acggggcctg ttgcgacccg agcggggcgt gcagcgtgtt
      121 ggctgcggcg gcctgcgcgg gcacctacat gggcgacggc accgcgtgca cgcccgaccc
      181 gtgttgcacg ggccagctca agggcgatgc caactgcgac ggcgtggtga acgtattcga
      241 tatcgatccg ttcgtggtgg cgctgacaag cctcgacgtg tggcagggca cctaccaggg
      301 agcgggatgc ggcttcctgt gcgttaacga ctgcaacggt gacggggagg tcaatgtctt
      361 cgatatcgac ccatttgtgc agtacctgac cggcgggtaa ccgccccgga caacggacaa
      421 ctgctccgat acgactttgc ggccgggcgc gcggcagcgt gcgcggccgc agctcgttgg
      481 gcatcgaacc ccaggctcgc tcaaccgctc gcaggcggcc agccgcgcgt aatcgccgct
      541 ttggccgata tggtaccctt ccgacggccg gcgccttcgg gtaaccttcg ctggccgaac
      601 gcccggcgcg ccaatcgctc gcgcccgccg aacggtcgat gagattggca gaaaaaagcg
      661 gcggttaatc gcgggaacgc cgggcccgcg tggagcctgc atgatcgaag tccaagaact
      721 gacaaaagtc ttccccaatc ccgacggctc cgagaaaact gccgtggaca acatcagctt
      781 ccgggtgctc gcccacgaaa tctatggcct gctgggcccc aacggcgcgg gaaaaactac
      841 cacgctccgc atggtcagcg ggctgctgcg cccgacctcc ggccgcgtcc tcatcaacgg
      901 caaggacgtc accaaccacg aagccgccgt caagcggcag atcggttatc tcacggccaa
      961 cacgggcctg taccagcgtc tcaccccgct ggaaattctc gagtacttcg cggtgcttta
     1021 cgacatcccg cgcccgcaag ctaagcagcg catcgccgaa ctcgtggact ggctcggcat
     1081 gcacgacttc atcaagctgc gctgcggcgc cctttcgacc ggccagaagc agcgcaccaa
     1141 catcgcccgc gccctgatcg ctgatccgcc agtgctaatc ctggatgagc ccacgctcgg
     1201 cctggacgtg ctcagcaatc gcctcatcct ccagtttatt caatctcaag ccgacgccgg
     1261 aaaagccgtc ctgctctcga cccacgccct cgatgagatc gagaagatgt gcggccgcat
     1321 cggcttgatc cacgatggcc ggctcatagc cgagaacgat ctcgaaggcc tcaagcggca
     1381 aaccgggcag agccggctga gcgacgcttt cctacacctg gtgcatgcga atgaaccgct
     1441 tctcgcggat tagcacaatt tggcgcaagg agttcgccga cacgctgcgc gaccgccgca
     1501 cgctggtggc catggtgctc gtgccgatgg tgctctaccc ggccctgatg cttggctcgc
     1561 tgcaggcgtt cgagcttcag gtcagcaagc tcaaacagca ggaatactcg atcgcggtcg
     1621 cgtccgatga agttcgccat tggcttcaaa cccgcattct cgatcccgat ctggcacgct
     1681 tcgcggccgc cgccggtctg cctgcggaag agttgcccgc cgccgttgag gccgccgaga
     1741 aagaggcgga tgcacaaagc ggcgacagcg ggcgcggcag gctcgaaaag gaagaggccg
     1801 ccgtccaccg ccgcccgccg ccgtaccggc tggaggtcgt cagtgatgta cgcctggcgg
     1861 tggcaaccgg catgaccaac gtcggcctgc tcgtcagtgg tgaactgctg cccacgccgg
     1921 attccgccgg cgcctgccag atcacgctgg tgatggataa ggccgaaatt cgcagccaat
     1981 tcgccgcgtc ggcccttgaa ggcgtgttca agcggctcag cgagcgcgtc gtcgcgcgcc
     2041 gccttcagga cctgcacctg acccgcgact atatcgagcc gtatcaaatc acccagctca
     2101 gcgtagccac gcccgaaaag ctgggcggct cgatcatcgg gcagatcgtc gccctgatcc
     2161 tggtcgtcat gacaatcacc ggcgcgatct atcctgccat cgacctgacc gcgggcgaac
     2221 gcgaacgcgg cacgctcgaa accttgatgg ccgccccggt gcccaaagtc gacctgattg
     2281 ccggcaagtt cgtcgtggtc gcctcgatcg gcatgctcag cgccgtgctg aatttgctct
     2341 caataggcgg caccgtctac ctgggaggcc tgggcgacgt ccttaccaag gagggcggca
     2401 tcacgatccc gctgcggacg ctgccgctgg tactggtcac gctcatcccg ctggccgtca
     2461 tgttcagtgc cgtgctgctc gccgtttgca gttttgcccg cagcttcaag gaagcccaga
     2521 actacatcat gccggtcatg gtcgtggcgt tgattcccgc cgtggtcgga ctcctgcccg
     2581 gcaccgagtt ggaaggcccg ctgctcatca tgccggtgac caacatcgtg atcctgacgc
     2641 gcgaactgtt catgggcgag atcaatcgcc tgggggtgct ctgggtcaca ctttcgacct
     2701 gcatttatgc cttggcggcg gtgggggtgg cggccaaact tttcgggcag gaggccgtcc
     2761 tgttcgccga cagcgcgtct atccggacgc tgctcaagcg caagttcttc aaaccgtcca
     2821 actggcccag cgcctcgcaa gctttgctgc tgatggcggt ggtctatctg ctgaacttct
     2881 tcatccagca gtcgcttttc caggccggcc tgacgcctgg ctcgccggag catctggcgg
     2941 gcttcgcgct cacgctggtg ctgctgctgg cggcgctgcc gatcggcgtg gcgctctaca
     3001 cgcgtgtacg catcaccacg gcattctcgt tttcaccgcc gcgctggcgt gggctcgcgg
     3061 ccggcctgtg catcgggcta tcgacctggg tgctggcgct ggtgtggatg tcgctccagg
     3121 agcgcatcct gccgctgccg ccggacttca aggaagccgc cgcgccctac gagcagatgc
     3181 tgcaacgcct ctcggtcgcc accgtgctat tcttcttcgc cattctgccg gccttctgcg
     3241 aagaactgtt cttccgcggc tatgcgctca gcggcctgcg gcacgtaatg ggcaaaaccg
     3301 gggcggtgct gctggtcgcg ttcgccttcg gcatgaccca catgtcgatt cagcgcctgc
     3361 cgctcacggc ggccgtgggc gctctgctgg gcctgctggt catacgctgg gggtcgatct
     3421 ggccggcggt cctggcccac gtgctgcaca acagcatcat cctgctcatc acgcgcgagg
     3481 atgggctgct gccgctgctg aagcgggccg ggttcgtcga tccggtcacc gaggatgtgt
     3541 ttccgccgct cccgtggatc gtcggggccg cggtcctgct gctggccggc ctggccctct
     3601 gcgtcaaacg ctcaagaccc gtcggccagc tcgcggacgg cctcggccaa agcggcggcg
     3661 atctgctcgg cgtcgccggc cagcagggtc cggcagtcaa agactagcgc ttcctcctgc
     3721 acgcggcaaa ttacgggcgt ctcgcgctgc cgcacggcgg ccgccagccg catgcttggc
     3781 agcgccgggt gccgaacgat cactatccag gtaggaaacg aaatcatcgg cagcgatcca
     3841 ccgccggctt cggaagtatc cggctgctgg gtgacttgtg cctcgggaag cacttggcga
     3901 atggcgtctg ccagttctac ggccgagttc tcgagctccg cgggcgatgc ggccagcatg
     3961 cggaaaaccg gcaagcgccg cgcgagggct tctgggtcgc gatagaggcg caaggtcgct
     4021 tccaagccgg ccagcgtcat cttgtccggg cggacggcac gggccagtgg gctctgacga
     4081 attcgcttga tgagatcagc gcggccgatg atcacgccgg cctgcggacc gcccagtagt
     4141 ttgtcgccgc tgaaaagcac gacgtccgcc ccgctcgcga cgctgtcgcg cacggccggc
     4201 tcatcccatt gcgacggggc tcgtcccgcc ggcgcctgcg tagtcgcggc ccaaccgctg
     4261 ggcgcctgcg cgatcaccgg cccgccaggt gcaccgccgc cttcgtccgg ccacggcaac
     4321 tcgcggtcga tcaggccact gccgaggtcg tcgatcacga gcaacccgcg gctgcgtgcc
     4381 agcttcacca agtcgtccaa cgacacgctc tcgacgaagc cctgtgtgcg atagttgctg
     4441 gtatgcacgc gcagcagcgc cacgctcgct tcactgatgg cccgctcgta gtcgtacagg
     4501 tgcgtgcggt tggtcgtgcc gacctcgatc atccggcagc cggccgcggc catgatgtcc
     4561 ggcattcggt acgagccgcc gatctcaacc aattgccccc tggagacgat cacctcgcgc
     4621 ccggccgtga gggccgcaag cgtaagaaac gtgccggcgg cgttgttatt cacgaccagc
     4681 gcatcttccg cgccggtcag ttcggccaat agctcgcgcg cgtgctcgtg gcggtcgccg
     4741 cgccgcccag tgggcaggtc cagctcgaga ttgcagtatc cggccgcgat gtcggcgata
     4801 gcgtcgatgg cttcctgggc cagcggcgcc cggcccaggc cggtgtgcag cacgatgccc
     4861 gtggcattga tgacgcgccg taagtgcggg cgggcgcgtt cgtgcagctt ctcgcgcacg
     4921 gccagggcga ggctgggggt atccacgtcc actttctcgc cgccgcgcaa cgcctcgcgc
     4981 cgctggttca gcacggcccg aatggcgtgc aacaactctt cgcgcgggaa ttcggccagc
     5041 agattcgcta cagccggcgc cttcagcaac tggtccacgg cgggcaggct gcgcatagcg
     5101 gcctggagcg gtccggcatc gggcggctgc atggggccgg gggggccggc gtccgtgctt
     5161 tgcggactcg aagcttcgtt gccggtcgga gccgaggcat cattttcgtg tgtatcgggg
     5221 gtgctcatgt cccgcatgat aggagag
//
LOCUS       DAOSYS010000249         5242 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13202_length_5242_cov_5.515520, whole genome shotgun sequence.
ACCESSION   DAOSYS010000249 DAOSYS010000000
VERSION     DAOSYS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13202_length_5242_cov_5.515520"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..871
                     /locus_tag="PLU99_13120"
     CDS             53..871
                     /locus_tag="PLU99_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOO18033.1"
                     /translation="MMAASAAHAGSACHAGPAFRVAFEYALSGDLAYLSHRDELRMLR
                     RALVRAGWPLRFSRGYNPIPWLSVPLPRRVGVASDCQLALTDLSEPRPAAWLRESLAA
                     ALPRDCRLGSLRAPAPGGTPHAQSAEYAVTLAWPAAQALAPAVERWRGILEMDERRCA
                     GGMSSPPAPKCQQFLEGVALEGPELRLRLRFIEQRTARPSEVLTELGLAPDAHEYTVR
                     LCEVAWNTQCSGPALESRASKGFAIGYKENHDESDEEQDHEENEKDSCERRLRN"
     gene            1086..>2544
                     /locus_tag="PLU99_13125"
     CDS             1086..>2544
                     /locus_tag="PLU99_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOO18034.1"
                     /translation="MAETAAPEPEQRAEPKPAHARTRPETAEAAARQMERQDEVLVIR
                     FPPVAPPPFEPRPVPAAEPESAAAPPASVAGREEPLEPAVTEELEPPPAPAPPETARQ
                     IGVKDADAEADLEADVVEEQAISEPIEPAAERDEELGPTADETPREPDGGEPPGFERR
                     GRRRRRRRRPNGHEGNGRAVQPPAEAPLRVEPQIRPRTPGGPQAAAPQEFTAPPGRHL
                     SPSQKRRLRRRRRSQENREPEPKAFETPAPPTAPAAEEAPAEEPAPPDFDENEGVALA
                     RIPGLGDEELPSLPSLDEGEEPAEEKAAAPAQTRATGREGRGRQRPGRRPVQEPEVEE
                     GGEAPVVGTKEMIINVVPGEECRIAILHKGKLEEMYIERASAESHVGNIYKGLVTNVE
                     PSIQAAFVDFGLGKNGFLHVSDLHPQYFPDGGQPAENVGRKLPRRSRPPIQRCLRRGQ
                     QIIVQITKEGIGTKGPTLSTYLSIPGRFLVMMPGMR"
     assembly_gap    2545..2644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2645..3790
                     /locus_tag="PLU99_13130"
     CDS             <2645..3790
                     /locus_tag="PLU99_13130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOO18035.1"
                     /translation="STYLSIPGRFLVMMPGMRQLGVSRKIEDDEVRIKLRSQLHELEL
                     PEGMGFIARTAAQDRTKRELQSDLKYLMRLWSMITRRMKEERAPAELYRESDLVIRTI
                     RDLYSAEIGKIVVDDAAVAQRAREFLQIFSPRAGDVVHEYTDPVPIFHRYGIEAELDR
                     LHSRHVPLRSGGSLVIEPTEALVAIDVNSGKFRTEDDAEATAFKINMEAADEIARQLR
                     LRDLGGVIICDFIDMRQESHKRAIERRLASNLRQHKERAKILRMSQFGIIEMTRQRQR
                     ASLTRSVYQDCRYCHGTGLVKTGESVALDVMRIIQLNIIRDPVRVIELTVSPEVANHL
                     LNRKRAALANLEAKYRRTILIRPEPNFGLDNVEVRCSDHRGRLIPPA"
     gene            3878..4837
                     /locus_tag="PLU99_13135"
     CDS             3878..4837
                     /locus_tag="PLU99_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOO18036.1"
                     /translation="MEPKQLGARLPQLVDRIVESYLADHRTQHIDKEFLPSTAAIIEI
                     CDMLMELTYPGYFGRMGLTRHNIAYHVGELLPRLWDQLSDQVHQALCHAAEQSGRLSE
                     DGQACRDKAVQIASDFLEAIPNIRAQLADDVQAAYDGDPAAFDTSECILAYPAVHAIT
                     IFRYAHHLYELDVPYVPRIMCEHVHRITGIDIHPGARIGRSFFIDHGTGVVIGETTEI
                     GDNVKIYQGVTLGALSFPKDERGRLIRGHKRHPTVGNNVTIYANAIVLGGETNLGAGS
                     IIGGSVFLTKSVEPGHQISITPPVLKVRPPQPAAPVTDMSPND"
     gene            4899..>5242
                     /locus_tag="PLU99_13140"
     CDS             4899..>5242
                     /locus_tag="PLU99_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOO18037.1"
                     /translation="MLSRDYRQIAATLSSTGTREQRMRAVVDALWDGLHASGVSWVGF
                     YLPEGETELVLGPSRNKPACSPIGLHGVCGQAFTQRKPMIVRDVRDLAQNYVACDPRD
                     QSEVVVPVFDENG"
BASE COUNT          996 a         1748 c         1622 g          776 t
ORIGIN      
        1 ttaatttcct gcgcgatgaa gcgcgcaaag ttctaccgcc ggaggctttc tgatgatggc
       61 ggcctccgca gcgcatgccg ggtcagcgtg tcacgcgggg ccggcctttc gcgtggcatt
      121 tgagtacgcc ttaagcggag acctggctta tctctcgcat cgcgacgaat tgcgcatgct
      181 gcgccgcgcc ctggtccggg ccggatggcc gctgcgcttt tcgcgcggat acaatcctat
      241 accgtggctc tcagtgcctc tgccgcggcg ggtgggcgtc gcttccgact gtcagttggc
      301 cctgaccgat ttgagcgagc cgcggcctgc ggcttggtta cgcgaatcgt tggccgcagc
      361 cctgccgcgc gactgccggc ttggtagtct gcgggccccg gccccgggcg gcaccccgca
      421 cgcacagagc gccgagtacg cggttacgct ggcatggccg gccgcccagg ccctcgctcc
      481 ggcggtagaa cgctggcgcg ggattttgga aatggatgag cggcgctgcg cgggcgggat
      541 gagctcgccc ccggcgccca aatgtcaaca atttctcgaa ggagtggctt tggagggacc
      601 agagttgcgg ttgcgcttgc ggtttatcga gcagcgcacc gcccggccct ccgaggtgct
      661 cacggaactt ggccttgccc cggatgcgca tgaatacacc gtccggctgt gcgaggtcgc
      721 atggaacacg cagtgctcgg gtccagcctt ggaatcccgg gcctcgaaag gatttgccat
      781 tggctacaaa gaaaaccacg acgagtcgga cgaagagcaa gaccacgaag aaaacgaaaa
      841 agacagttgc gagcgccgac tccggaactg agacacaact aactgaaacg ggcggcccgg
      901 ccaccgcacc tcccgctgcg accaagccaa agacgcggcg caaagcgacc aagaaagtca
      961 cgacgcgccg gcgcaagact gcggcagtgg agacggtcgc cggcgagact ccccttgccg
     1021 agcagcaaga aaccgcagtc gccgaacttc aggaagcgcc gcctgcgcca ggagtttcgg
     1081 gtcccttggc cgagacggcc gcgcccgagc ctgagcaacg agcggaaccg aagcccgcgc
     1141 acgcccgaac tcggcctgag acagccgaag ccgctgctcg gcagatggag cggcaggacg
     1201 aagtgctcgt catccgcttt ccgccggttg cccccccacc cttcgaaccg cggccggtcc
     1261 cggcggctga accggaaagc gccgccgcgc cgccggcaag cgttgccggg cgcgaggagc
     1321 cattggagcc ggccgttact gaggagctcg agcctcctcc ggcgccggcc ccccctgaga
     1381 cggcccggca aattggcgtt aaagatgccg atgccgaagc cgacctagaa gccgacgtgg
     1441 tcgaggagca ggcgatcagc gagcccatcg agccggccgc tgagcgcgac gaagagctcg
     1501 ggccgaccgc ggacgagacg ccgcgcgagc ccgacggcgg tgagccaccg ggctttgaac
     1561 ggcgtggacg tcggcgccgg cgccggcggc gaccgaacgg tcacgaaggc aacgggcggg
     1621 ccgtacagcc gccggccgag gcgcctctgc gagttgagcc gcagattcgt ccgcgcacac
     1681 ccggcggacc acaggccgca gcgcctcagg agttcacggc gcctcccggg cggcatcttt
     1741 cgcccagcca gaagcgccgg ctaaggcgcc gccgccgctc ccaagaaaat cgcgagccgg
     1801 agccgaaggc ctttgaaacc cctgctccgc ccacggcccc ggcggctgaa gaagccccgg
     1861 ccgaggagcc ggccccgccc gactttgacg aaaacgaagg cgtcgcactg gcgcgaattc
     1921 ccggcttggg cgacgaagaa ctgccttcac tgccgtcgct cgatgagggc gaagagcccg
     1981 ccgaggaaaa ggcggccgcc ccggcccaaa cccgcgccac aggccgcgaa ggccgcgggc
     2041 gtcagcgccc cggccggcga ccggtccaag aacctgaagt tgaggaaggc ggggaggctc
     2101 cggtcgttgg caccaaggag atgatcatca atgtggtgcc cggcgaggaa tgccgcatcg
     2161 ccattctgca caaaggcaag ctcgaagaga tgtacatcga gcgggcctcg gccgaaagcc
     2221 acgtgggcaa catctacaag ggcctggtga cgaacgttga acccagcatt caggccgctt
     2281 tcgtcgactt cggcctgggg aagaacgggt tcttgcacgt atcagatctg cacccgcagt
     2341 acttcccgga cggcgggcag ccggcggaaa acgtggggcg caagctgccg cgccgcagcc
     2401 ggccgcccat tcagcgctgc ctgcgccgtg ggcagcagat catcgtgcag atcaccaaag
     2461 agggaatcgg caccaagggc ccgacgctgt cgacgtacct gagcatcccg ggccgcttcc
     2521 tggtgatgat gcccggcatg cgtcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnntcgacg tacctgagca tcccgggccg cttcctggtg atgatgcccg gcatgcgtca
     2701 actcggcgtg tcgcggaaga tcgaggacga cgaggtgcgc atcaagctcc gcagccagct
     2761 ccacgaattg gagctgccgg agggcatggg ctttatcgcc cgcactgccg cccaggaccg
     2821 caccaagcgc gagctgcaaa gcgacctcaa atacctcatg cgcttgtgga gcatgatcac
     2881 gcggcggatg aaagaggaac gtgctccggc cgagctctac cgcgagtccg acctggtcat
     2941 tcgcactatc cgcgatctgt atagcgccga gatcgggaag atcgtggtgg atgatgctgc
     3001 ggtggcccag cgggcccgtg aattcctgca gatattcagc ccgcgcgccg gcgatgtggt
     3061 ccacgagtac accgacccgg tgcctatctt ccaccgctac ggcatcgagg ccgaactcga
     3121 ccgcctgcac tcccggcacg tcccgctgcg ttccggcggg tcgttggtga tcgagcccac
     3181 cgaggccctg gtggccatag acgtcaacag cggcaagttc cgcacggagg acgacgccga
     3241 ggcgaccgct ttcaagatca acatggaggc cgccgacgag atcgcccgcc agctccgcct
     3301 gcgcgatctg ggcggtgtga tcatctgcga cttcatcgac atgcgccagg aatcgcacaa
     3361 gcgcgccatc gaacgccgcc tcgcgagcaa tctgcggcag cacaaggagc gggccaaaat
     3421 cctgcgcatg agtcagttcg gcatcatcga gatgacgcgg cagcggcagc gcgccagcct
     3481 gacgcgcagc gtttaccagg attgccgcta ctgccacggc accggcctgg tcaaaaccgg
     3541 cgaatcggtc gccctggacg tgatgcgcat cattcaactg aacatcatcc gcgaccccgt
     3601 acgcgtgatt gaactgaccg tttcgcccga agtggccaac catctgctga accgcaagcg
     3661 ggccgcgctg gcgaatttgg aagccaagta ccgccgaacg attctcatcc gcccggaacc
     3721 gaacttcggc ttggacaatg tggaggtgcg ctgctccgac catcgcgggc gcttgattcc
     3781 gccggcgtga tgcaaaacaa cgtgcgtcga ggacgccgca cgaaagcgtg cgtcctcgac
     3841 gcaccgttgc ctccttgcct cgttgcctga gatcattatg gaacccaaac aactcggcgc
     3901 aagactgcct caattggtcg atcgcatcgt ggaaagctac ctggctgacc accgcactca
     3961 gcacatagac aaagaattcc tgccgtccac cgcggccatc atcgaaatct gcgacatgct
     4021 gatggagctg acctatcccg gctatttcgg ccggatgggc ctgacgcgcc acaacatcgc
     4081 ctatcacgtg ggcgagttgc tgccgcgctt gtgggaccag ctcagcgatc aggtacacca
     4141 ggcgctctgc catgccgccg aacagtccgg gcggctgtcc gaagacgggc aggcctgccg
     4201 cgacaaggcc gtgcagatcg ccagcgattt tctcgaggcg atacccaaca tccgcgctca
     4261 actcgctgac gatgtacagg ccgcttacga cggcgacccg gccgccttcg acacctccga
     4321 gtgcatcctg gcctacccgg ccgtccacgc cataaccatc ttccgctacg cccatcacct
     4381 ctacgagctg gatgtgccct acgtgccacg catcatgtgc gagcacgtac accgcatcac
     4441 gggcatcgac atccaccccg gcgcccgcat cggccgcagc ttcttcatcg accacggcac
     4501 cggcgtggtc ataggcgaaa ctactgagat cggcgacaac gtgaagatct accagggcgt
     4561 gacgctcggg gccctttcgt tccccaaaga tgaacgcggc cgcctgatcc gtggccacaa
     4621 gcggcatccg acggtcggga acaacgtgac gatttacgcc aacgccattg tgctgggcgg
     4681 cgaaaccaac ctcggggccg gctcaatcat cggcggctcg gtgttcctta cgaagagcgt
     4741 tgagcccggc caccagattt cgatcacgcc gccggtgctc aaagtccgcc cgccgcagcc
     4801 ggccgcgccc gtaaccgaca tgagccccaa cgactgaagt cgcgacggac tccacgctta
     4861 gccgaaactg aagaaaccca tgcgtagccg agagattcat gcttagccga gactatcgcc
     4921 agatcgcagc caccctctca agcaccggca cacgtgagca gcgcatgcgc gccgtggtcg
     4981 acgcgctttg ggacggcctg cacgcaagcg gcgtctcctg ggtgggcttc tacttgcccg
     5041 agggcgaaac cgaactcgtg ctcggcccga gccgcaacaa gcccgcgtgc tcgccgatcg
     5101 gcctgcacgg cgtctgtggc caggccttca cgcaacgaaa gccgatgatc gttcgcgacg
     5161 ttcgcgacct cgcgcagaac tacgtcgctt gcgacccgcg cgaccaatca gaggtcgtgg
     5221 tgccggtctt tgacgaaaac gg
//
LOCUS       DAOSYS010000250         5231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13235_length_5231_cov_4.720634, whole genome shotgun sequence.
ACCESSION   DAOSYS010000250 DAOSYS010000000
VERSION     DAOSYS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_5231_cov_4.720634"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /gene="rho"
                     /locus_tag="PLU99_13145"
     CDS             complement(<1..1184)
                     /gene="rho"
                     /locus_tag="PLU99_13145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOO18038.1"
                     /translation="MAKATTRTRTKKKLQDVIEAMPEEVEAPVAPAPEPAEPVAEERP
                     PEKPAAYESPIDEMEDDGPQPLTNTREKVKQSPQRPAEDDRQHKRRRRDENSTAIDRE
                     THEKYERVKRGELHITDLQKMTVAELHEVAKTEGIEDFVGLPKQDLIFQILKRRIAQN
                     GLLYGEGVLEILPDGFGFLRSPEYNYLPCPDDIYVSPSQIRRFGLRNGHIVAGQIRPP
                     KESERYFALLRVEAINFESPEAVTEKTNFEDLTPLHPDERLFLETTKTELNMRIVDLV
                     TPIGKGQRMLIVAPPRTGKTVLLQKMANAISTNHPDAIVIILLIDERPEEVTEMERAT
                     KAEVVSSTFDEPASRHVQVAEMVIDKAKRLVEYGRDVVILLDSITRLARAYNTEVPHS
                     GK"
     gene            complement(1245..1970)
                     /gene="coaE"
                     /locus_tag="PLU99_13150"
     CDS             complement(1245..1970)
                     /gene="coaE"
                     /locus_tag="PLU99_13150"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOO18039.1"
                     /translation="MSPEFNAAFCFFSRPCVIFRAGRVGNSMESTKPIIGLTGGIGAG
                     KSTVAAEFGRQGCLVIDSDSLNREVLRRPEVLARLRQWWGPDVVNEQGLPNVARIAEI
                     VFADPASRKRLEELTHPLIAQMRADIIKAVREDQAVKAVILDSPLLIESNLDRLCHAI
                     IFVEASEAQRLQRVNASRKWTEEQLRGREKWQTALEVKRARADFIIDNDGPPDRLRPQ
                     VSVILEEITSRFKGGPLSSASRG"
     gene            complement(2165..3931)
                     /locus_tag="PLU99_13155"
     CDS             complement(2165..3931)
                     /locus_tag="PLU99_13155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO18040.1"
                     /translation="MNETTATGMAVVQDTIHDPYKPSPWPVLLLAMALFIVPLIVLSQ
                     FIAYWRTDVVDDQMFGYFGWRIAHGATVYLDVWDNKPPGIYWINALGMLLGRDSYLGV
                     IGMCSLALLVSFACFFGICASLYYRGAAAFSTALLGFYLTHGYFTGGTNRTETFLVAC
                     ELAAVLLYIRGFARDRGWKWLLAGLLCGCAFLFKQTGLAACAAMMMHLTLLAITRDIT
                     WRQWLRRGLLVGCGVGLTLAVAAGYLAWQGALGEALFATFGFNRTYFAAGASGFPYKY
                     LTYYLLKNHLFPILLLPMLMAIAACIHAVLWRLRPYYRPREIEESLTALRPACPRYMV
                     LFVLWFLWSVWGALLSPHAFRHYLVPTIPPLMLIASYLINMLQAEMRLLVVMQRRAWA
                     TAAVVVIAYFGFDALERQFEQFSMVWVPRFLQHKPAEWEVLGEAVKTVTGPEDKIQCL
                     GYYPGVYLWSRRTNAVRFTTTEKVGQVRHHADFVLKELQEKLPAEPPTVIVISVSDYL
                     KVQGPAKPDDRAPTMLLGPWIDENYERLADVPGPNTYIFKRKDLVQPSDRELQKKVWQ
                     MTEHGIAAAFDAPVPAPRIVGA"
     gene            complement(3944..4702)
                     /locus_tag="PLU99_13160"
     CDS             complement(3944..4702)
                     /locus_tag="PLU99_13160"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOO18041.1"
                     /translation="MSALTRREVIGATGAAIGGWLLVDGLPAMAQQAPDTQAPPPAEG
                     EEPTGPYVLPPLPYGYADLEPYISAETLKLHHDKHHAAYVKGANNAFAQLEKIRREGG
                     DAIKRVRCVTEDLAFNASGNILHTLYWNSMKKDGGGDPPAGGELAKMLLRDFGTLEAF
                     RGQFNAAAVQVQGSGWAILALEPVSQRLVVLAAEKHENQTIWGCVPLMVLDVWEHAYY
                     LQYQNRRADYIKAFAELIDWGGVQERLQRALKNS"
     gene            complement(4771..5124)
                     /locus_tag="PLU99_13165"
     CDS             complement(4771..5124)
                     /locus_tag="PLU99_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOO18042.1"
                     /translation="MKVKRLVNEPQKEVTLPGAAGVRMRMLVGPAEGAGVFHMRHFEV
                     APGGHTPHHEHDYEHEVLILKGVCSVKGADGDKACQAGDVVWMPPNERHQFVNTGREP
                     LEFICLIPAPKECSL"
BASE COUNT          978 a         1800 c         1465 g          988 t
ORIGIN      
        1 atcttgcccg agtgcggcac ttcggtgttg taggcgcggg ccagacgcgt gatcgagtcg
       61 agcaggatca ccacgtcgcg cccgtactca accaaccgct tggccttgtc gatgaccatc
      121 tcggccacct gcacgtgccg gctggccggc tcgtcaaagg tcgagctgac gacctcggcc
      181 ttggtggcgc gctccatttc ggtcacttcc tcgggccgct cgtcgatcag caggatgatg
      241 acgatggcgt ccgggtggtt cgtggaaatg gcgttggcca tcttttgcag caggaccgtc
      301 ttgccggtgc gcggcggggc cacgatcagc atgcgctgac ctttgccgat cggcgtcacc
      361 aggtcgacga tgcgcatgtt cagctcggtc ttggtggttt cgaggaacag gcgctcgtcc
      421 gggtgcagcg gggtcaaatc ttcaaagttc gtcttttcgg tcaccgcctc gggcgactcg
      481 aagttgatcg cctccacccg cagcagcgcg aagtaacgct ccgactcctt cggcgggcga
      541 atctgccccg cgacgatgtg gccgttacgc aggccgaagc ggcgaatctg cgaggggctc
      601 acatagatgt cgtccgggca aggcaggtag ttgtactccg ggctgcgcag gaagccgaaa
      661 ccgtcgggca gaatctccag cacgccctcg ccgtacaaaa ggccgttctg cgcgatccgc
      721 cgcttgagaa tctggaagat caggtcctgc ttgggcaggc cgacgaagtc ctcgatgccc
      781 tcggtcttgg caacctcgtg cagctcggcc acggtcatct tctgcagatc cgtaatgtgc
      841 agctcgccgc gcttgacacg ctcgtacttc tcgtgcgttt cgcgatcaat cgccgtgctg
      901 ttctcgtcgc gccgccgccg cttgtgctgg cgatcgtcct ccgccggccg ttgcggcgac
      961 tgtttgacct tctcacgggt gttggtgagc ggctgcggcc cgtcgtcctc catctcatcg
     1021 atgggcgatt cgtaagcggc gggcttttca ggcggacgtt cttcggcgac cggctcggcc
     1081 ggttcagggg cgggggctac aggggcctcg acctcctccg gcatggcctc gatgacatcc
     1141 tgcagcttct tcttggtacg agtgcgtgtg gttgcctttg ccatttcaat agactccaga
     1201 tatggacccc ggcgactctc gtccgggaca tgttgccgat tttgtcagcc ccggctggcc
     1261 gaactcaacg gccctccctt aaagcgggag gtgatttcct caaggatgac acttacctgc
     1321 ggtcgcaacc ggtccggggg gccatcattg tcaatgatga aatccgctcg cgcccgcttt
     1381 acctccaaag ctgtctgcca tttttcccgt ccccgcagct gttcttccgt ccacttgcgg
     1441 gaagcattca cccgctgcaa tcgctgtgcc tcgctcgcct caacgaaaat aatcgcgtga
     1501 catagacgat cgaggttgct ttcgatcaga agcggggagt caaggatgac ggctttgacc
     1561 gcttggtctt ctctaaccgc cttaataata tccgctcgca tctgggcgat caaggggtgc
     1621 gtcagctctt ccagacgctt ccgcgaagca gggtcggcaa aaacaatctc cgcaatccgg
     1681 gccacgttcg gcaggccctg ctcattcacc acgtccggac cccaccactg ccgcaagcgc
     1741 gccagcacct ccggccgccg caacacctcg cggttcaagc tgtctgagtc gattaccagg
     1801 cacccctggc gtccaaactc ggcggccacc gtggatttac ccgccccgat cccaccggtt
     1861 aaaccaatga tcggtttggt tgattccatg gaattaccga cccgcccagc cctgaatatt
     1921 acgcatggcc gcgaaaaaaa gcagaacgcg gcgttaaatt cgggggacac tcctgtaccg
     1981 ggccgccgac ccagcgcgct gggcggtttt cggttaaacg caccggatag tctgccgacc
     2041 caacccagcc ctgtcaaccc gtcagcgcac ccgccctgaa tgccccgcca ccaagccccg
     2101 cttcaggggt tttgtccggc cgcgagcccc gccaaagcct ccccgctttt attgccggct
     2161 acgactaagc gcccacaatc ctcggcgccg ggacgggcgc gtcaaaagct gccgcgatgc
     2221 cgtgctcggt catctgccac actttcttct gtagctcgcg gtccgaaggc tgcaccaggt
     2281 ctttacgctt aaaaatgtat gtgtttggac ccggtacatc cgccaggcgc tcgtaattct
     2341 cgtctatcca tggccccagg agcatcgtcg gcgcacggtc gtccggctta gccgggcctt
     2401 gcaccttcag gtagtcgctc accgaaatca cgatcaccgt cggcggttcc gccggcagct
     2461 tctcttgcag ttccttcagc acgaaatcgg cgtgatggcg gacctggcca accttctcgg
     2521 tcgtcgtaaa acgtaccgcg tttgtacgcc gcgaccacag atacacgccg gggtagtaac
     2581 ccaggcactg tatcttgtcc tccggccccg tcaccgtctt caccgcctca cccagcactt
     2641 cccattctgc cggcttgtgc tgaaggaaac gtggcaccca caccatcgaa aactgttcga
     2701 attggcgctc gagcgcgtca aagccaaagt acgcgatcac caccaccgcc gctgtcgccc
     2761 aggcccgtcg ctgcatcacc accagcagcc gcatctccgc ctgcagcatg ttgatcaaat
     2821 acgaggcaat cagcatgagc ggcggaatcg tcggcaccag gtagtgccga aacgcgtgcg
     2881 gactgagcag cgccccccaa accgaccaca gaaaccacaa cacgaacagc accatgtaac
     2941 gcgggcacgc cggccgcaac gccgtcaacg actcctcaat ctcgcgcggg cgatagtacg
     3001 gccgcagccg ccacagcacc gcgtgaatgc aggccgcaat cgccatcagc atcggcagca
     3061 gcagaatggg gaacaggtga ttcttcaaca gatagtacgt gaggtacttg tacgggaagc
     3121 cgctcgcccc cgccgcgaag tacgtgcgat taaagccaaa ggtcgcaaac aacgcctcgc
     3181 ccaaggcccc ttgccaggcc aggtagccgg ctgcgaccgc cagcgtcagc ccgacgccgc
     3241 agcccaccag caacccgcgc cgcagccact ggcgccacgt tatgtcacgc gtgatggcca
     3301 acagcgtcag gtgcatcatc atggcggcgc aggccgccag gccggtttgc ttgaacagaa
     3361 acgcgcagcc gcacagcaac cccgccagca gccacttcca gccgcgatcc cgcgcgaagc
     3421 cgcgtatata caacagcacc gccgccagct cgcacgccac gaggaaggtc tcggtacggt
     3481 tcgtcccccc tgtgaagtac ccgtgcgtca agtagaagcc cagcagagcc gtagagaacg
     3541 ccgccgcgcc gcgataataa agggaggcgc atatgccgaa aaagcaggcg aagctgacca
     3601 gcaaggccag cgagcacatg ccgatcacgc ccaggtagct gtcgcgcccc agcagcatgc
     3661 ccagcgcgtt aatccaatag atgcccggcg gcttgttgtc ccacacgtcc aggtagaccg
     3721 tcgcgccgtg cgcgatgcgc cacccgaagt acccgaacat ctggtcgtca actacatcgg
     3781 tgcgccagta cgcgatgaat tgcgacagca cgatcagcgg aacgatgaac agcgccatcg
     3841 ccagcagcag caccggccac ggcgagggtt tgtatggatc gtggatcgtg tcctgcacca
     3901 cggccatgcc cgtggcggta gtctcgttca tgttcagctc aactcacgag ttcttcaaag
     3961 cccgttgcag gcgctcctgt accccgcccc agtctatgag ctccgcaaag gctttgatgt
     4021 agtcggcccg ccggttctga tattgcaggt aataggcgtg ttcccagaca tccaaaacca
     4081 tcaatggtac gcaaccccag atcgtctggt tttcgtgctt ttcagccgcc agcaccacca
     4141 gccgctgcga aaccggctcc agcgccagta tcgcccagcc gctgccctgc acttgcaccg
     4201 cggccgcgtt gaactgcccg cggaacgcct ccagcgtccc gaagtcgcgc agtagcatct
     4261 tggccagttc gccgccggcc ggcgggtcac cgcccccgtc cttcttcatg ctgttccaat
     4321 acaacgtgtg caggatgttg cccgaagcgt tgaacgccag gtcctcagtc acgcagcgca
     4381 cgcgcttgat ggcatcgccc ccctcgcgcc gaatcttctc gagctgcgca aacgcgttgt
     4441 tagccccctt cacgtacgcc gcgtgatgct tgtcgtggtg cagtttcagc gtctccgcgc
     4501 tgatatacgg ctccagatcc gcataaccgt acggcaacgg cggcaacaca tacggaccgg
     4561 tcggctcttc accctcggcc ggcggcggcg cctgcgtgtc cggcgcctgc tgagccatag
     4621 ccggcagacc atcaaccagc aaccagccgc cgattgcggc gcccgtggcc ccgatgacct
     4681 cacgacgagt cagcgctgac atttcggcct cctgtcgcca cggctgggag ggtcaggctt
     4741 ccgccgagcc gacctcagcg tggcaagctg tcacagcgag cattccttcg gagccggaat
     4801 caaacaaatg aattccaacg gctcgcgacc ggtattcaca aactggtgcc gctcgttcgg
     4861 cggcatccag accacgtctc ccgcctggca cgccttgtcg ccgtcggccc ctttgaccga
     4921 acagacgcct ttcagaatca ggacctcgtg ttcgtaatca tgttcgtgat gcggcgtgtg
     4981 cccgcccggc gccacctcga agtgccgcat gtgaaacacg cctgcaccct cagccggccc
     5041 gaccagcatg cgcatgcgga cgccggccgc acccggcagc gtcacttcct tctgaggttc
     5101 atttactagt cgttttacct tcatagcagg atcatccctg agcagcggtc atcccatgct
     5161 atccgagctc actcgagtat tcccaggcga cgcgggccgg cgctgcttcg cattcagcct
     5221 ccattactca c
//
LOCUS       DAOSYS010000251         5205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13311_length_5205_cov_5.878252, whole genome shotgun sequence.
ACCESSION   DAOSYS010000251 DAOSYS010000000
VERSION     DAOSYS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13311_length_5205_cov_5.878252"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLU99_13170"
     CDS             <1..738
                     /locus_tag="PLU99_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO18043.1"
                     /translation="QVKTPLITDWSEVESVRPPVEALDLDVARINGFCRQTDRLVLGG
                     CCPRPVERLQFLCGAERVYVGLARRVPELLGLLRTVHDFYTRELEAWARTDVDGLMIM
                     DDWGGQHGMLVSPAVWRTLFKPLYADYIDIAHRHGKSLWMHSDGHILDILPDLVELGL
                     DAINCQVACMGVAALGERFRGRITFWGELDRQHLLPHGTRAEVIAAVREMKAALYQNG
                     GLIAQCEFGPGAQPDNVAAVFEAFSDA"
     gene            750..2858
                     /locus_tag="PLU99_13175"
     CDS             750..2858
                     /locus_tag="PLU99_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOO18044.1"
                     /translation="MHIYGLHVLDLAIVVLYVAAIVWIGKRVARRTKSTDDFYVAGRK
                     LGGFYQFFLNFGTSTSADQAVAVARETYRQGIGGMWIQFLVLFLTPFYWFTTLLFRRC
                     RLITIGDFFAERYGSRFLAAAFAVFTLTMAFIGGGVSYMVAGKMVLALTPKPESAYTE
                     HERIAVAQFNEYQQLKAQLAQGLSPAQQARYEELNERAKRGELLSFVSYLDRRWIYVV
                     YAIVVATYIGLGGFSAAAIADALQGFLILIFSIALIPLGLSRIGGFAGLHASVPDYMF
                     RVFGSAATSEYAWYTIVAMSLANLVSIIAGAPMMATAGSAKNEMTARVGMLGGMFCKR
                     IIMLFWALAGLLAIGLYAGRVHDADLIWGYMTRDLLFPGGVGLMLTGVLAASMSALGA
                     QAVTNSALFVRNLYQPLAPNRSEAHYIRVGRFVIIAVLLGGIITALYADNLLNLFKYF
                     ISVPAVFGAAIWLGFIWRRVTKWAVILQVLFCFALYAIIPNVFPLLDCVRRNPAFLRE
                     TQPRTITLATSALAEDVAAGRAECVGQTINKPHVMEPAGVFFEKVVRIDPADPHSPKI
                     GQGRFQAEIWLLSWFGLDFSGCSKAQLVAIRFLVDALLPFVLLFLLSVVTRPVPKLHL
                     DLFFARLHTPVQPTPEAEVAALSQAASQPDMYDRGKLLPRSNWEVMKPTWLDLVGFGG
                     SWILVGIVLLLLWLMVTIGR"
     gene            2863..5160
                     /locus_tag="PLU99_13180"
     CDS             2863..5160
                     /locus_tag="PLU99_13180"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOO18045.1"
                     /translation="MYVRIVFCAIVVFVAAFPARAEAPATPRQAYLSLAESAYQQGLE
                     TCPADVERWLKEWKPTVEWGYAPPASPIYLAGLAGSLYDLTKNEDYAEVAAHWLANQH
                     RFKESCPEHVIKYRPEYGDGLPTMTDFFHLPVFAQAYLHIKDSPAVTAERRAHIEASI
                     ADSADYIRNFPEWGPMNRTMLRAQGLVLAAKALPDHPHAAFWRKLGDILARDSWGQWE
                     EEDAQIYHPIWFLALMHYADAVGDPSLYAQHQVRYYCDYFVHLLSPAGMVPDFGDARW
                     NENWAGYLTCLERGAREYRSPQLKWGAQRIFDAMTEQYGSKIGLAAGLTLTYAYRWAD
                     DSITPAAPPARSEEVLEELIGKKIVFRNGWGPDSTYLLLNYRDEGPFARVARDFLRLT
                     IPAEEERMHHAHSDENSICLFMNQGSVLLHDAGYRDTMPSGPYGAYRADYFHNRLVAR
                     NDKRDREQPLYEFLRNSGAYRSTQTEKIDFLTAPGAEMSRTRLTDQRTGYVADRVIVR
                     LTKDDIFLVFDIAKVLRPEYYTFATLWHGTTVLGQGPQYYITAVDAIGKYSPPQTRAL
                     LVQFLQSGVRTDGTFPIKRYWQDETAVYQAISSHYRAGQVETFVTALVPHARGADVQP
                     LLDSIKLLDVEKPRNGLGVQVQCGDEVHYICVKTDLDMDILPDNVRPRYSFDSGRVKY
                     GPIETDASFAYARRSGDSLTYAAANMVKVIFDGRTVFAGRTATFALQPDDLSTGYGPP
                     KWRYWEDTVDLSQPEPAAEAASRWP"
BASE COUNT          926 a         1684 c         1579 g         1016 t
ORIGIN      
        1 caggtcaaga cgccgcttat cacggattgg agcgaggtcg agtccgtgcg cccacccgtt
       61 gaggcactgg acctggacgt ggcgcgcatc aacggtttct gccgccagac ggatcgttta
      121 gtgctcggcg gctgctgccc acggcctgtt gagcggctgc aattcctgtg cggtgcggag
      181 cgtgtctacg tggggctggc gaggcgcgtg ccggaactgc tcggactgtt gcggacggtg
      241 cacgatttct acacgcgcga gctggaggcc tgggcgcgca ctgacgtcga cgggctaatg
      301 atcatggacg actggggcgg ccagcacggc atgctggtct cgcccgccgt gtggcgcacg
      361 ctcttcaaac cgttgtacgc cgactacatc gacattgccc accggcacgg caaatcgctc
      421 tggatgcact cggatgggca tattctggat attctgccag acctggtgga gctcggcctg
      481 gacgccatca attgccaggt ggcctgcatg ggggttgccg cgctgggcga gcgtttccgc
      541 ggccggatta ctttctgggg cgagttggac cgccagcacc tgctgccgca cgggacgcgc
      601 gcggaagtaa tcgccgcggt gcgtgagatg aaagccgccc tgtaccaaaa cggcgggctg
      661 attgcacagt gcgaattcgg cccaggcgcc cagcccgaca atgtcgcggc ggtcttcgag
      721 gccttcagcg acgcgtaaag gagagagtgt tgcacatata tggcctgcac gtcctcgacc
      781 tggcaatcgt ggtcctatac gtggccgcca tcgtctggat cggtaagcgc gtcgcgcggc
      841 gcaccaagag cactgatgat ttttacgtcg ctggccgcaa gctgggcggc ttctaccagt
      901 tctttctgaa cttcggcact tcgaccagcg ccgatcaggc cgtagccgtg gcgcgtgaaa
      961 cttaccgcca aggcatcggc ggcatgtgga tacagttcct ggtcctgttc ctgacgccct
     1021 tctactggtt caccacgctg ctctttcggc gctgccgtct gatcacgatc ggcgactttt
     1081 tcgcggagcg ctatggcagc cgcttcctgg ccgccgcctt cgccgtattc acgctgacta
     1141 tggctttcat cggcggcggt gtgagctaca tggtggcggg caagatggtg ctggccctga
     1201 cgcccaagcc ggagagcgcc tataccgaac atgagcgcat cgcagtcgcg cagttcaatg
     1261 agtaccagca gcttaaggcc caactggcgc agggtctcag ccccgcgcag caggcccgtt
     1321 atgaggagtt gaacgagcgc gccaagcgcg gcgagttgct gtcgttcgtc tcttacctcg
     1381 accggcgctg gatctacgtt gtgtacgcaa ttgtagtcgc cacatatatc ggattaggcg
     1441 gcttctccgc cgcggccatc gcggacgcgc tgcaaggctt tctcatcctg atcttctcca
     1501 tcgccctgat cccgctgggg ctgagccgta tcggcggctt cgccggactg cacgcctccg
     1561 tgccggacta catgtttcgc gtcttcggct cggccgcgac cagcgaatac gcttggtaca
     1621 cgatcgtcgc catgtcgctc gcgaatttgg tgtcgatcat cgccggcgcg cccatgatgg
     1681 ccaccgccgg ctcggccaaa aacgaaatga ccgcccgcgt cggcatgctc ggcggaatgt
     1741 tctgcaaacg catcatcatg cttttctggg cgctggccgg cctgctggcg atcggcctct
     1801 atgccggacg cgtacacgat gccgacctta tctggggcta catgacgcgc gatctgctgt
     1861 tccccggcgg cgtcggcctc atgctcacgg gcgtgctggc ggccagcatg tcggccctgg
     1921 gcgcgcaggc ggtgaccaac tcggcgcttt ttgttcgcaa tctatatcag ccgctggcgc
     1981 cgaaccgctc agaggcccac tacatccgcg tcggacgttt tgtcattatc gcagttctgc
     2041 tcggcggcat catcactgca ctgtacgccg acaacctgct gaacctcttc aagtacttca
     2101 tctctgtgcc ggcagtgttt ggcgcggcga tctggctggg cttcatttgg cggcgtgtga
     2161 cgaaatgggc cgtcatattg caggtgctct tctgcttcgc actctacgcc atcatcccca
     2221 acgttttccc gctgctggat tgcgtgcggc gcaacccggc gttcttgcgc gaaacgcagc
     2281 cgcggaccat cactctggca acctccgcgc tggccgaaga cgtcgccgcc gggcgcgccg
     2341 agtgtgtcgg tcagaccata aacaagccgc acgtgatgga acccgccggc gtgttcttcg
     2401 aaaaggtggt gcgcatcgac ccggccgacc cgcactcgcc caaaatcgga cagggccgct
     2461 ttcaggccga aatttggctg ttgagctggt tcgggcttga cttctcgggc tgcagcaaag
     2521 cgcaactggt ggccatccgt ttcctggttg acgccctgtt gcctttcgtg ctgctgttct
     2581 tactgagcgt cgtgactcgt ccggtgccca agctgcacct ggatctcttc ttcgcgcgac
     2641 tgcacacccc tgtgcagccg acaccggagg ctgaggtcgc ggctctctcc caggccgcct
     2701 cacagcccga catgtatgac cgcggaaaac tgctgccgcg ctcgaactgg gaagtgatga
     2761 agccgacgtg gctcgacctg gtcggcttcg gcggaagttg gatactcgta ggcatcgtgc
     2821 tgctgctgct gtggttaatg gtaacgatcg gcaggtgatc tgatgtacgt aagaatagtg
     2881 ttttgtgcca ttgtagtctt cgtagcggca ttcccggccc gagccgaggc gcccgcaacg
     2941 ccgcgccagg cgtacttgag cctggcggag tccgcctatc aacaaggcct ggaaacgtgc
     3001 ccggccgacg tcgagcgctg gctgaaggaa tggaagccca cggtcgagtg gggctacgcg
     3061 ccgcccgcca gtcccatcta cctggccggt ttggccggct cgctgtacga cctcacgaaa
     3121 aatgaggatt acgcagaggt ggccgcgcat tggctggcca accagcaccg cttcaaggag
     3181 tcctgtcccg aacacgtgat caagtaccgc cctgagtatg gcgatggctt gccaaccatg
     3241 acggactttt ttcacctgcc ggtcttcgcc caggcgtatc tgcacattaa ggacagtccg
     3301 gccgtaacgg cggagcggcg ggcacacatt gaagccagca tcgctgacag cgctgattac
     3361 atccgcaact tccccgagtg gggcccgatg aaccgcacca tgctgcgcgc ccagggcctg
     3421 gtgctggcgg ccaaggccct gccggatcat ccgcatgcgg ccttctggcg caaactgggc
     3481 gacatcctgg cgcgcgacag ttggggacaa tgggaagagg aggacgccca gatttaccat
     3541 cccatctggt tcttggcgct catgcactat gccgatgcgg tcggagatcc cagtttgtac
     3601 gcccagcacc aggtgcgcta ctactgcgat tatttcgtcc atttactatc cccggccggc
     3661 atggttccgg atttcggcga tgcgcgctgg aatgagaact gggccggcta tttgacctgc
     3721 ctcgaacgcg gggcgcgcga ataccgcagt ccacaactca aatggggtgc gcagcggatt
     3781 ttcgacgcca tgaccgagca gtacggctca aagatcggcc tggccgccgg cctgaccctg
     3841 acctatgcct accgctgggc ggatgacagc atcacgcccg cagcgccacc ggcccgcagc
     3901 gaagaagttc tggaggagct gatcggcaag aaaatcgtct tccgcaacgg ctggggcccc
     3961 gactcgacgt acctgctgct caattatcgc gatgaaggcc cattcgcccg cgtggcgcgc
     4021 gatttcctgc ggctcaccat cccggccgag gaagagcgca tgcaccacgc ccattcagac
     4081 gagaacagca tctgcctctt catgaaccag ggcagtgtgc tactgcacga cgccggctat
     4141 cgcgacacca tgccgtccgg accgtacggg gcgtaccgcg ccgactactt ccacaaccgg
     4201 ctcgtggccc gcaatgacaa gcgcgaccgt gagcaaccgc tgtacgaatt tctgcgcaac
     4261 tcgggcgcct atcgttcgac ccagaccgaa aaaatcgact tcctgaccgc tcccggcgcc
     4321 gagatgagcc gcacccgcct gaccgaccag cgcaccggct acgtcgcgga ccgcgtcatc
     4381 gtccgcctca cgaaggacga cattttcctg gtgttcgaca tcgcgaaggt actgcgtccg
     4441 gaatactaca ccttcgccac attgtggcac gggacaacag tgctcgggca gggaccgcag
     4501 tattatatca ccgcggtcga cgccatcggc aaatacagtc ctccgcagac gcgcgcccta
     4561 ttggttcagt tcctccagtc cggcgtgcgc accgacggca cgttcccgat caaacgctac
     4621 tggcaggatg agaccgccgt ctaccaggcc atctccagcc actatcgcgc cggccaagtg
     4681 gagacatttg taactgcgct agtcccgcac gcgcgcggcg ccgacgtgca gccgctgctc
     4741 gactccataa aactgctgga tgtcgagaaa ccgcgcaatg gtctgggagt gcaggtgcag
     4801 tgcggcgatg aagtacacta catttgcgtc aagactgacc tggatatgga catcttgccg
     4861 gacaacgtgc gcccgcgcta ctcatttgac tccggccgcg tgaaatacgg gccgatcgaa
     4921 acggacgcca gcttcgccta cgcccggcgt tcgggcgact ctctgacgta tgcggccgcc
     4981 aacatggtca aggtcatctt cgacggccgc accgtgttcg ccggccgcac cgcaaccttc
     5041 gcgctccagc cggatgatct ctcgaccggt tacggcccgc ccaagtggcg ctactgggaa
     5101 gataccgtgg acctcagcca acccgagccc gcagcggagg cggcgtcacg ctggccctag
     5161 cggcatacaa tcagcaacgt gtgggccact gcatgaagcg cagct
//
LOCUS       DAOSYS010000252         5194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13350_length_5194_cov_5.161315, whole genome shotgun sequence.
ACCESSION   DAOSYS010000252 DAOSYS010000000
VERSION     DAOSYS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13350_length_5194_cov_5.161315"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="PLU99_13185"
     CDS             <1..1254
                     /locus_tag="PLU99_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOO18046.1"
                     /translation="RRQAILQQLVRQVEQRREELAQVLTIEVGKPIKDARGEVTRLID
                     TLRIAAEEAVRIGGEWLPLDISPRAEGYQGLVRRFPIGPCAFITPFNFPLNLAAHKIG
                     PAIAAGCPWILKPASATPISALLLGEMVAQTDWPKAAFSILPCSGAEAEPLVTDERIR
                     KLSFTGSAKVGWDIRARAGRKRVTLELGGNAACIVDRGADLDYAADRITLGAFYQSGQ
                     SCISVQRVLIHRDIYDALVPKLVERARRLELGDPADEKTALGPLISLQDAQRVEQWIA
                     QAVTAGAKILCGGRRHGPCVEATYVADVDPRQPLSCQEVFGPVAVLARFDDFATAIRM
                     ANDSRYGLQAGVFTRDLEHAWHAYEELEVGGVVINDVPSTRVDSMPYGGVKDSGLGRE
                     GVRYAIEEMTERRVMVLKREKNPHR"
     gene            1437..1751
                     /locus_tag="PLU99_13190"
     CDS             1437..1751
                     /locus_tag="PLU99_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOO18047.1"
                     /translation="MSKKLYVGNLGYNVSSSDLEALFAAHGTVQSAQVIQDRESGRSK
                     GFGFVEMESSEAAEAAIAALNGQVHGGRALTVNEAKPRPDRGGGRGGYGGGGGRGGRS
                     RY"
     gene            1878..2081
                     /locus_tag="PLU99_13195"
     CDS             1878..2081
                     /locus_tag="PLU99_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18048.1"
                     /translation="MNTKWQGEPKQWRVRGKSKDGLRVTLGSYDTESEAQADSAKFIS
                     EGYYRDITVERIERAPGPPAPPA"
     gene            complement(2089..3723)
                     /gene="rny"
                     /locus_tag="PLU99_13200"
     CDS             complement(2089..3723)
                     /gene="rny"
                     /locus_tag="PLU99_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOO18049.1"
                     /translation="MLTTTYPLAEAGTGTVVLMLLLGLALGGAATWAYTWWQANRETG
                     RQRAAAAKLKADAEQQAADILKAAEVTAQKKHIEALQRFETETAETRAELKAAERRLA
                     KREDALDKKLEVLTTKERKIELAEQRLAEREREIEQKNAAADEALIQQRNELLRIARL
                     TPEQAKQLCLQRIESEVEREAGELVDRIISHAEENAREKARYITITAIQRYAAEHTCE
                     TTVATVDVPSDDMKGRIIGREGRNIRAFEKSTGATVIVDDTPGIVSVSCMDPVRKEVA
                     RLSLDRLIRDGRIHPTRIEEIVAEVTKEVDQRIIEAGKAAAAEVNVQGIHKKIIETLG
                     RLQYRTSYGQNVLRHSIEVAFLCGMMADELGLEGPLARRCALLHDIGKALDHEHEGAH
                     TRTGYEFARRFNEPPAVLNAIIGHHGDVAATHPYTPLVAAADAISAARPGGRRESLER
                     YIKRLQDLENIATSFSGVDLAYAIEAGREVRVIVDASTVDDSAALKLARDVANKIEQD
                     MVTFPGEIKVTVLRELRATEYAISGRHRSNGSANVS"
     gene            4327..4770
                     /locus_tag="PLU99_13205"
     CDS             4327..4770
                     /locus_tag="PLU99_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18050.1"
                     /translation="MNTDRHSNQPVLWLIAIALSAIALGLWLKPGQSMLPAALAQVTP
                     LAGARGVYAFTGQLDANRYGLFMLDIDQGTIWCYEFDNVNGARKLRLSAARTWIYDRY
                     LKDFNSAPPDFRMVQQLVAQQRAQDAPASTPALALPEPTPDEQHR"
     gene            4781..>5194
                     /locus_tag="PLU99_13210"
     CDS             4781..>5194
                     /locus_tag="PLU99_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO18051.1"
                     /translation="MAKPFYSIEEVCEKLGKTQDQVRKLVREGSLREFRDAGKIFFKA
                     EDVDQLAGSGKKTPEQFEPLLDAVDDKPEVPEVPEIPELATPSLPSGGTSIIGLEPIE
                     EEKKEDTVITASGIGVFDDDELEIDADPMAATQITT"
BASE COUNT          943 a         1597 c         1663 g          991 t
ORIGIN      
        1 cggcggcaag ccatcctgca acagctcgta cggcaggtcg aacagcgacg cgaagagctg
       61 gcgcaggtgc tgaccatcga agttggcaag ccgatcaaag acgcccgcgg agaagtgacg
      121 cggttgatcg acacgctgcg catcgccgcg gaagaggcgg tgcgaatcgg cggcgagtgg
      181 ttgccgttgg acatcagtcc gcgagccgaa ggctaccagg ggctggtgcg ccgcttcccc
      241 atcggcccgt gcgctttcat cacgccgttc aatttcccgc tgaacctggc cgcccacaag
      301 atcggccccg cgattgccgc cggctgtccg tggattctca agccggcttc cgcgacgccg
      361 atcagcgcct tgcttctggg tgagatggtg gcgcagacgg actggcccaa ggcggctttt
      421 tcgatcctgc cgtgcagcgg ggcggaagcc gaaccgctgg ttaccgacga gcgaatccgg
      481 aaactaagct tcaccggttc ggccaaagtc ggctgggaca tccgcgcccg ggccggccgc
      541 aaacgcgtga cgctcgagtt gggcggcaac gcggcctgca tcgtcgatcg cggcgcggat
      601 ctggactacg cggccgaccg catcacgctg ggcgctttct atcagtccgg ccagagctgc
      661 atcagcgtgc agcgcgtgct catccaccgg gatatttatg acgctcttgt tccaaagctg
      721 gtcgagcgtg ccagacggct cgaactgggc gatcccgcgg atgagaaaac cgcactgggg
      781 ccgctcatct cgctgcagga tgcgcagcgc gtcgagcagt ggatcgccca agccgtgacg
      841 gccggcgcca aaattctttg cggcggccgg cggcacggcc cctgcgtgga ggctacctac
      901 gtcgcggacg tcgacccgcg gcaacccctg agctgccagg aagtcttcgg accggtggcc
      961 gtgctggcgc gcttcgatga tttcgcgacc gccatacgaa tggccaacga cagccgctac
     1021 ggcttgcagg ccggcgtttt cacgcgcgat ttggagcatg cttggcacgc gtacgaggag
     1081 ctcgaagtcg gcggcgtggt catcaatgac gttccgagca cccgcgtgga cagcatgccc
     1141 tacggcggcg tgaaggacag cggcctgggg cgcgaaggcg tgcgctacgc catcgaggag
     1201 atgaccgagc gccgcgtcat ggttctgaag cgagaaaaaa atccgcacag gtgaattttt
     1261 ttctccggtg gcctacaata tcggcggttg gttcgctgaa gctcgcgtta cctgacgtgt
     1321 gttcaagtcc cgattgctga gagcgcttcc ttccttcccg gccggaactt gcggccgctt
     1381 tcaacgaccc tgaaagcaaa agatcgggtc aacaatgcca tcgataggag aaaacgatga
     1441 gcaagaaact gtatgtgggt aacctgggct acaacgtcag cagctccgat ctggaggcgc
     1501 tgtttgcggc gcacggaacg gtgcagagcg cgcaggtgat ccaagaccgc gagagtggta
     1561 gaagcaaagg ttttggtttc gtagagatgg agtcgagtga ggccgccgag gccgccattg
     1621 ctgccttgaa cgggcaggtg cacggcgggc gggctctgac ggtaaatgag gccaagccgc
     1681 gtccggatcg cggcggcggt cgtggcggct acggcggcgg gggcggccgt ggcggtcgca
     1741 gccggtacta atacgagcaa aaaccactga aaggggaggc cggacgtccg tcctccccct
     1801 ctttatacaa ttttgcgcag cgacgagctt tgccgcccaa ggcccggcac acgggcgcgg
     1861 cgttccagga ttcgggcatg aacacaaaat ggcagggtga acccaagcag tggcgcgtac
     1921 gcgggaagtc gaaggatggt ttgcgcgtga cgctcggcag ctacgacacc gagagcgaag
     1981 cgcaagcgga ctccgcgaag ttcatttccg aaggctacta ccgcgatatc accgtcgagc
     2041 ggatcgagcg ggctcctggg ccacccgcac cgccggctta agtgcaaatt acgacacgtt
     2101 cgcggagccg ttagaacggt ggcggccgct aatggcgtat tcggtggcgc gcagttcgcg
     2161 cagcaccgtc actttgattt cgccggggaa cgtcaccatg tcctgttcga tcttgttggc
     2221 gacatcgcgg gccagcttca gagccgcaga atcgtccacc gtcgaggcgt cgacgatcac
     2281 gcgcacttcg cggccggcct cgatagcgta cgccagatcg acgcccgaga acgaggtcgc
     2341 gatgttttcc aggtcctgca ggcgcttgat gtaacgctcc agcgattcgc gccggccacc
     2401 gggacgcgcc gcgctgatgg cgtcagccgc ggccaccagc ggcgtatacg ggtgcgtggc
     2461 cgccacgtca ccgtgatgcc cgatgatggc attgagcacc gccggcggtt cattgaagcg
     2521 ccgcgcgaac tcataaccgg ttcgcgtgtg ggccccctcg tgctcgtgat cgagagcctt
     2581 accaatatcg tgcagcaggg cgcagcgccg cgccagcggg ccttccagcc ccagctcatc
     2641 ggccatcatg ccgcatagga acgcgacctc gatgctgtgc cgcagaacat tttggccgta
     2701 gctggtgcga tattgcagtc ggcccagcgt ctcgatgatc ttcttgtgta tgccctgcac
     2761 gttgacttca gccgcggcgg ccttgccggc ctcgatgatc cgctggtcga cctccttggt
     2821 cacctcggcc acgatctcct cgatgcgcgt tgggtgaatg cggccgtcgc gaatcaggcg
     2881 gtccagcgac aggcgggcaa cttccttgcg caccgggtcc atgcaggaga cgctcacgat
     2941 ccccggcgtg tcatcgacga taaccgtcgc gccggtcgac ttttcgaacg cccggatgtt
     3001 gcgcccctcg cggccgatga tgcggccttt catgtcgtcg gaaggtacgt cgaccgtggc
     3061 cacggtagtt tcgcaggtgt gctcggcggc gtagcgctga atggcggtga ttgttatgta
     3121 gcgggccttc tcgcgcgcgt tctcttcggc atgcgagatg atccggtcga ccaattcgcc
     3181 cgcttcacgc tcgacttcgc tttcgatccg ctgcaggcag agctgtttgg cctgctccgg
     3241 cgtgagccgg gcgatccgca atagctcgtt tctctgctgg atcagggcct cgtccgcggc
     3301 cgcgttcttc tgctcgattt cgcgttcgcg ttcggccagt ctttgctcgg ccagctcgat
     3361 cttgcgttcc ttggtcgtca ggacctcgag cttcttgtcc aaggcgtctt cgcgcttggc
     3421 caaacgccgt tcggcggcct ttagttccgc gcgggtttcg gcggtttcgg tctcaaagcg
     3481 ctgcaaagcc tcgatgtgtt ttttttgtgc agtgacctct gcggccttga gaatgtcggc
     3541 ggcctgctgc tcggcgtcag ctttaagctt ggcggcagcg gcgcgctggc ggccggtctc
     3601 ccggttggct tgccaccagg tgtaggccca ggtggcggcg ccaccgagcg ccagtccgag
     3661 taacagcatc agcacaaccg tgccggtgcc ggcttcggcc aaagggtatg ttgtggtaag
     3721 catttttcat ccgcttgtct tgtctgttgt aactacaagc ggggcgacgg tttgcgcgcc
     3781 ccggactccg ggcgcgatgg tgggcgaact ctgagattcc gattcgccgg ggcgaatcgg
     3841 tgcattcagc aagccagcaa cacggcggct gcgagactgg tgttgatgtc aggggttccg
     3901 cgaaaggcgc ttatgatcca gcaagtttgt tatgaaccat taagtatgat gttgcgcctt
     3961 gttctcgaac ccggttgagc ggtcacacgc tcaacgtatt atcaaaaccc cggacgttcc
     4021 agtaacgcac cgaagccgcg aggtccagcc gcgtcgtgcg gcggtctggg cgtatcgcgc
     4081 aaccatcggc gcctaaacgc tctacgtacg cttagtacgt gtcggcagta taagaggttc
     4141 ttcattatct tgccagcggg ggcgcctgaa taagagaata aagtgaaaat gctcgcaacg
     4201 gatataaaag gttcgctatg gcggccggtc gggcctcggc cccgcgcctt gctggccaca
     4261 acctccgtcg ctaccatgct ttgcggcagg acgggcggat agggaaccga ggcgctattg
     4321 gcggctatga acactgaccg gcacagcaac caaccagttc tgtggctgat cgctattgcc
     4381 ctttcggcca tcgcgttggg cctgtggctt aaacccggcc aaagcatgct gccggcggcc
     4441 ttggctcagg tcaccccatt ggcgggggcc cgcggtgttt acgccttcac cggccagctc
     4501 gatgccaacc gttacgggct gttcatgttg gacatcgacc agggcactat ctggtgctac
     4561 gagttcgaca atgttaacgg ggcgcggaag ctgcgcctta gcgcggcccg gacctggatt
     4621 tacgaccggt acctcaagga tttcaacagc gcgccgcccg actttcgcat ggtgcagcaa
     4681 cttgtcgcgc agcagcgtgc ccaggacgcc cctgcgagca cgcccgctct cgcactgccc
     4741 gaacccaccc ctgatgagca acatcgctag gagcgattga atggcgaaac cgttttactc
     4801 catcgaagag gtctgcgaga agctcggaaa aacccaggac caagtccgta agctggtccg
     4861 cgagggtagc ctgcgcgaat tccgcgatgc cggcaaaatc ttcttcaagg ctgaggatgt
     4921 cgatcaactg gccggcagcg gcaagaagac gcctgagcag tttgaaccgc tgctcgacgc
     4981 ggttgacgac aaacctgagg tgcccgaggt ccccgagatc cccgagttgg ccacgccttc
     5041 gctcccgtcc ggcggaacca gcatcatcgg ccttgagccg atcgaggaag agaagaaaga
     5101 ggacacggtc atcaccgcca gcggcatcgg cgtgttcgat gacgacgaac tcgaaatcga
     5161 cgccgatcct atggccgcca cccagatcac gacc
//
LOCUS       DAOSYS010000253         5129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13548_length_5129_cov_4.745566, whole genome shotgun sequence.
ACCESSION   DAOSYS010000253 DAOSYS010000000
VERSION     DAOSYS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13548_length_5129_cov_4.745566"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLU99_13215"
     rRNA            complement(<1..853)
                     /locus_tag="PLU99_13215"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1405..3417
                     /locus_tag="PLU99_13220"
     CDS             1405..3417
                     /locus_tag="PLU99_13220"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOO18052.1"
                     /translation="MAKRVVLPALFLVTFISGGCLPPPGPSKQAGNPKLIPFDSEAAL
                     LSYFQQQAIEQTRPQRRRGWLFGRIPTLAPGAAEDNAVNAAPTDGTGGSTAYSTTNLQ
                     EAGVDESDVFKSDGTYFYIAREQSLRIVQASPPEALAEIANVDLGAWIDALYLRDKTI
                     IALGSQADGARVGAAAPDGAEIMIWPPYYPRAKISVIQIDISDPANPAITHRLELDGC
                     LVSSRLTGGRLIVVLTVVPELPAQPTPREINRMTLTEVMPKARASGGASADAVPWSNW
                     LRPEAPDGCAMTAVLTLDAGNVETIVESAAVLANAGTIYASPEALYLTDSAYDPDDNY
                     RENTVIHKFSFGEDGAARYTASGSVPGRLLNQFALSDHKGNLRVATHRVGNGGVFWDD
                     VVVGVAVAESGNTSSSDDAPPPGTAQDRADTQNAPQEPDNAVFVLGESNGALDILGSV
                     ANFGTNEQIYAVRFLGDHGFVVTFRQIDPLFVLDLSDPQDPRLVGELEMPGYSEYLHP
                     LGEDYLIGIGRATDENEWGGTSVKGIQLSLFDVSDWTNPQVVQQLSLGSWGSYSEISA
                     THKAFTFIERDGQTLIALPAVLTSEGGTYYYSYEFAGVLCYSVDPTAGFTKLGEVAAV
                     GRPIGWWPQWQRAAFIGNELYALTPDGIRAAPLTDFNATEQITLSE"
     gene            3705..5120
                     /locus_tag="PLU99_13225"
     CDS             3705..5120
                     /locus_tag="PLU99_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOO18053.1"
                     /translation="MTLRKVLKKWDIRAGSVGALHLVMLAAVVCTARANPPANIPNAG
                     QTPATKPAANMGAQVQTEDVNALKQLIEQKVAEQSPNAAQTQPNAAQPSAKQASSRPR
                     MLNKPQPIVLNKGAAMMATDAPPGPEGERPSLGLTPPPADQPQPRWSCAKDSIEKPAI
                     WNGKAATYTFTAKNEGEGPLNIEIKGCCGSKISGSKSRTIQPGQTEDIEVTIATSGRE
                     GEFTRKIRVQTNDVNNPQVELTCKGRVLAAFKADPKVVNFGTIPRDAGPVTKTVTLTR
                     GDGGPLHPKVAACEGHASAELREIEPGEKYTLDITVSPPWPNDKVRGSVMIETGLEEA
                     PQDSVRYFGAVEARLRAEPRALSLPAGEERPTELRTRLVWAGAPGRALAAVASDPALQ
                     AKIVEEDGQQFVVLSVPAGYELPAKKPASVSVTTDDPVADSLRIPISAGAAAREPARP
                     ATLMPTGAANKATQSAPATQR"
BASE COUNT         1029 a         1692 c         1505 g          903 t
ORIGIN      
        1 gtttacggcg tggactacca gggtatctaa tcctgtttgc tccccacgct ttcggatctc
       61 agcgtcagaa tcggcccagt gccccgcttt cgcctctggc gttcctctag atatctacgc
      121 atttcaccgc tccacctaga gttccaggca cccctaccga cctcaagcta cgcagtttgc
      181 ccagcaattc cccggttgag ccgggggctt tcacaagaca cttgcgcagc cgcctacacc
      241 cgctttaagc ccaatgattc cgaacaacgc tagcctcctc tgtcttaccg cggctgctgg
      301 cacagagtta gccgaggctt cctttgggtc tgctcaacgc ggcggattat tcacccgctt
      361 tgcttgcgaa accctgacag cagtttacat cccgaaggac ttcctcctgc acgcggcatt
      421 gctgggtcag gcttgcgccc attgcccaag attcgttact gcagccaccc gtaggtgtcc
      481 gggcagtgtc tcagtcccga tgaggcgggc cacgctctca cgcccgctac ccgtcgtagc
      541 cttggtgagc cattacctca ccaactagct gataggacat gggccgctcc aagaccggca
      601 ggccggttac ccggtccccg ccctttggac gccaggccat gcgacccggc gacttcatcg
      661 ggcattagca gcactttcgc taaagaacct tgcggtccgc tgttattccc gtgtcaagga
      721 tacgttaccc atgcattacg ctcccgtccg ccactgaacc gaccgcagtt acccacggcc
      781 agtcccgtac gacttgcatg tcttagccat gccgccagcg ttcgttctga gccaggatca
      841 aactcttcga aaagaatttg agaaaccgcc gccgttaggc gagcggtccc attttccggc
      901 ttaggctcaa acactcacgt ctgcatcgag aatgttcgag caacgttcgt cggtttacgc
      961 cgccacacca aagcaacggc accgacgatc tttgctgcgg atcccggcgc gccagcccat
     1021 tctgcagagg gccttcacgc cggcacattc tcgcggcttc gtccactgtt cacttttcaa
     1081 agatcgcccg ctctttcgag cggggctccg tataatacca ggtttctccc cctttgcaac
     1141 agggaaacgc actcgtcata cagaaaattc cgtcccggac ccgaaaaaag tgcttcaaag
     1201 ccgaaaaaat ctcccggccg ctcaaaaaat gggatattat cggccgctaa acagcctctc
     1261 gacctgacac ctgtaataca cctacaaact tacagcgcaa ccaagcccga aaatcggagc
     1321 ttaacgcctt gtaacgacgg gcccgcctcg aactccagcc cgtcaaaatt ccagccttcc
     1381 ggctttgacc caggaggttc ggctatggcg aagcgagtgg tcctgcctgc gttgtttctc
     1441 gtcacgttca tttcgggcgg ctgtcttccg cctcccggac cgtccaagca ggccggcaac
     1501 cctaaactca tcccgttcga ttccgaggcc gccctgctct cctactttca gcagcaggca
     1561 atcgaacaaa ctcgcccaca aaggcgccgc ggctggcttt ttggcagaat tcccacgctg
     1621 gcgccaggcg ccgcggaaga taacgccgtg aacgctgccc ccactgatgg aaccggcggt
     1681 tcaaccgctt actcgaccac gaaccttcaa gaagccggcg tcgatgagag cgacgtcttc
     1741 aaaagcgacg gtacgtactt ctacatcgcc agggaacaat ccctgcgaat cgtgcaggcc
     1801 agcccgcctg aggcgctggc cgaaatcgcg aatgtggacc tcggcgcgtg gatcgatgcg
     1861 ctctacctgc gggacaaaac gatcatcgcg ctcggcagtc aggccgacgg cgcccgtgtc
     1921 ggcgccgccg ctcctgacgg cgccgaaatc atgatctggc ccccctacta cccgcgcgcg
     1981 aagatcagcg tgatccagat cgacatctcg gatcccgcca accctgccat cacgcaccgc
     2041 cttgagctcg acggctgcct ggtttccagc cggttgaccg gcgggcgcct gatcgtggtg
     2101 ctgaccgtcg tgccggagct gccagcccag cccacaccgc gcgagatcaa ccggatgacg
     2161 ctcaccgagg tcatgcctaa ggcgcgtgcg tcgggcggcg catccgcgga cgccgtgccg
     2221 tggtccaact ggctgcgccc ggaagcgccg gatggttgcg ccatgacggc cgtgctcacg
     2281 ctcgacgccg gcaacgtcga aacgatcgta gagtcggccg ccgttctggc caatgcaggc
     2341 acgatctacg cctcgcccga ggcgctgtac ctcactgatt ccgcgtacga cccggacgac
     2401 aactaccgcg aaaacaccgt aatacacaaa ttcagcttcg gtgaagatgg cgccgcgcgc
     2461 tacaccgcca gcggctcagt ccccgggcgg ctgctgaacc agttcgcgct gagcgatcac
     2521 aagggcaacc tgcgagtggc cacgcaccgc gttggcaacg ggggtgtctt ctgggacgac
     2581 gtcgtggtcg gcgtggccgt cgccgagagc ggcaatacgt cctccagcga cgatgccccg
     2641 ccgcccggga ccgcgcagga ccgggccgac acgcagaacg ccccgcagga accggacaac
     2701 gcggtcttcg tgctcggcga gagcaacggg gcgctggaca tcctcggctc ggtcgcgaac
     2761 ttcggcacca acgagcagat ctacgccgtt cgcttcctgg gcgatcacgg cttcgttgtc
     2821 acgttccgcc aaatcgaccc actcttcgtg ctggacctgt ctgacccgca ggacccccgc
     2881 ctcgtgggtg agctggagat gccgggttat agtgagtacc tccaccccct cggcgaggac
     2941 tacctcatcg gcatcggccg cgccacggac gaaaacgagt ggggcgggac gagcgtcaag
     3001 ggaattcagc tctcgctgtt cgacgtgagc gactggacca atcctcaggt cgtccagcag
     3061 ctcagcctcg gaagctgggg cagctattcg gaaatcagcg cgacgcataa ggccttcacg
     3121 ttcatcgagc gcgacggcca gacgttgatc gcgctgccgg ctgtgctgac ctccgaaggc
     3181 ggcacctatt actacagtta tgaattcgcg ggcgtgctct gctacagcgt cgatccaact
     3241 gccggcttta ctaagctggg agaagtcgca gcggtcggcc ggccgatcgg ctggtggccg
     3301 caatggcagc gggctgcctt catcggcaat gagctgtacg ccctgacgcc ggacggcata
     3361 cgcgccgccc cactgactga tttcaacgct acggaacaga taacgctctc cgaatgattg
     3421 gggaacgcga ggggttagtt gggcacgctg gcgggccgac gggctttgcc agcgtgcccc
     3481 aatttttggt gtcgcatgaa gagcgacagc cggctgacgc tcgaacgccg gcctgtgggg
     3541 tcagccgata ttttcggcta acttgtcgac ctgccttgtg tccaaccatc tagctgggta
     3601 attggtgtga cccggatctt gtcgacgagt tcgtgccagg gcgcgcccgg agtgcgggcg
     3661 gctctggttg tgtttgtagt acggcttcca aaaaggagaa ccggatgaca ttgcgaaagg
     3721 tgctgaagaa atgggacatt cgagcgggct cggtcggagc ccttcacctg gtgatgctgg
     3781 cggcggtggt gtgcacggct cgggccaacc cgccggcgaa tatccccaat gccggtcaaa
     3841 cgccggccac caagccggca gctaatatgg gggcacaagt ccaaacggag gacgtgaacg
     3901 cgctcaaaca gctcatcgaa cagaaggtgg cggagcagag tccgaacgcc gcccaaaccc
     3961 agcctaacgc ggctcagccg agtgccaaac aggcctccag ccgccccaga atgctcaaca
     4021 agcctcagcc aattgtcctg aacaaggggg cggcgatgat ggccaccgac gcaccgcccg
     4081 gcccggaagg agagcggccg tccctgggcc tgacgccgcc gcccgccgat caaccccagc
     4141 cgcgatggtc ctgcgcgaag gactcgattg agaagccggc aatctggaac ggcaaagccg
     4201 cgacgtacac tttcactgcc aagaacgaag gggaagggcc gctgaacata gaaattaagg
     4261 gctgctgcgg ctcaaaaatc tccggctcga agagccgcac cattcagccg ggtcagacag
     4321 aggatatcga agtcactatc gcgacaagcg ggcgcgaggg cgagttcacg cgcaaaatcc
     4381 gtgtacagac caacgacgtg aataatcctc aggttgaact gacgtgcaaa gggcgcgttc
     4441 tggccgcgtt caaggccgat ccaaaagtcg tcaatttcgg cacaatcccg cgcgacgccg
     4501 gcccagtgac aaagaccgtc accctgacgc gcggcgacgg cggccccctg catcccaagg
     4561 tcgcagcgtg cgaggggcac gcctcggcgg aattgcgcga aattgaaccc ggcgaaaagt
     4621 acacactaga catcacggtc agcccgccct ggccgaatga caaggtgcgt ggttcggtca
     4681 tgatcgaaac aggcctcgag gaggcgccgc aggattccgt acgctacttc ggcgcggttg
     4741 aagcccgcct tagagctgag cccagggcgc tgtcgctgcc ggccggagaa gaacgcccga
     4801 ctgagctgcg cacgcggctg gtgtgggccg gcgcgccggg gagggccctg gccgcggtcg
     4861 ccagtgaccc ggccctgcag gcgaagattg tcgaggaaga tgggcagcag ttcgtcgtgt
     4921 tgagcgtgcc tgcgggatat gaactgccgg cgaagaaacc cgcgtcggtt tccgtgacta
     4981 ctgacgaccc ggtggccgac agcctgcgca ttccgatttc tgccggcgcc gcggcgcgcg
     5041 agcccgcgcg tccggctacg ctaatgccga ccggcgcggc gaacaaggcc acgcaatctg
     5101 ccccagcgac acaacgttag ggtgaaagt
//
LOCUS       DAOSYS010000254         5121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13572_length_5121_cov_4.397158, whole genome shotgun sequence.
ACCESSION   DAOSYS010000254 DAOSYS010000000
VERSION     DAOSYS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13572_length_5121_cov_4.397158"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLU99_13230"
     CDS             complement(<1..577)
                     /locus_tag="PLU99_13230"
                     /inference="COORDINATES: protein motif:HMM:NF016635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOO18054.1"
                     /translation="MAKTLRVHTLAKELGVTSKEVIDKCRAEGVELKNHMAAISAGLA
                     ESIREWFSAGQDVTSIEVGDRVDVSKVRRSRKKAAEPTAENGEAAVAVAEPSESQAPE
                     FPETVPEAPEAEKPPQPEVPAAEEAAPEPPPPAPEPQPEISPAPPAEVPAEPVQEPVA
                     TPPPPAPPTRPAPTLLPPPPPPPPVKPAGPQV"
     gene            1072..2379
                     /locus_tag="PLU99_13235"
     CDS             1072..2379
                     /locus_tag="PLU99_13235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010853844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOO18055.1"
                     /translation="MKVFMLGWEFPPFISGGLGTACYGLTKGLDHLGTEIIFVLPRGP
                     EKLQLETSPTTSQAASQRRPEAFRYTRFRTVDAVIRPYVSPGVEDEPEPATVGARRAK
                     VRAKGPAERVRIIAASSSEGPGGHYGGDLFYEVERYAGLACEIAASEGDFDLVHAHDW
                     MTYPAGIAVADMTGKPLVVHVHSTEFDRSGVHVNQRIYDIERAGMTRAQRVICVSHFT
                     RSVVLQHYGVDPRKVDVVYNAVEENGTPESRTLPPIAHDEKIVLFLGRITMQKGPEYF
                     LAAAKRVLEVMGNVRFIMAGSGDMTRRMIELANQMGIGHKVLFTGFLRGDDVRRVFRM
                     ADLYVMPSVSEPFGIAPLEALANDVPVLISKQSGVSEILSHALKVDFWDVDEMANKII
                     AVLRHPPLQATLRDHGSIEVRRLSWVDSARSCLKVYETAVSPA"
     gene            2397..3683
                     /locus_tag="PLU99_13240"
     CDS             2397..3683
                     /locus_tag="PLU99_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015264594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOO18056.1"
                     /translation="MASVCFYFQVHQPFRLRRFSVFDRSSDYFDDERNRQICRKVAEK
                     CYLPANAVIEELATRYAGRFRVAYSLTGCVIDQFRLYAPEVLESFQRLARTGAVEFLS
                     ETYYHSLSLLYSREEFAEQVALHRRTVQELFGQTPRVFRNTELIYSNDLARIAAQMGY
                     NVVLCEGADNLLGYRSPTYLYTAPDGIPVRLLLKNYRLADDIAFRFGNKNWSEWPLTA
                     DKFARWVDQINGHGYVVNLFMDYETFGEHQWAETGIFEFLRHMPSELFKLPGNDFKSP
                     SECAATYPASGEYDVPHLISWADTERDLSAWLGNAMQSNALHELYKLEPAVKACGDPT
                     ILRDWRRLQSSDHFYYMCTKFLADGTVHQYFNPYDSPYDSYINFMNVLDHLRSRLPKQ
                     DAEPAPRPKASRAKSTTAAPTVKAGRKPRSQKPKAS"
     gene            complement(4045..4308)
                     /locus_tag="PLU99_13245"
     CDS             complement(4045..4308)
                     /locus_tag="PLU99_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOO18057.1"
                     /translation="MSRTWGKRLRASATPAGGGRRACLGRKIVGMRHILVHAYYNIDV
                     DAIWRAVKTSLSALVGELEAALRSWDKEDQQSAPGASEGRDPV"
     gene            complement(4448..4756)
                     /locus_tag="PLU99_13250"
     CDS             complement(4448..4756)
                     /locus_tag="PLU99_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOO18058.1"
                     /translation="MIAERHGLRIDPDEIAAFCRRHGIVRLSLYGSILRDDFGPDSDV
                     DVLIEFPPGGTPSLLDLGGMQVELTSLLGREVDLKTPGFLSPPILRRVLAERQMQYAA
                     "
     gene            complement(4810..5110)
                     /locus_tag="PLU99_13255"
                     /pseudo
     CDS             complement(4810..5110)
                     /locus_tag="PLU99_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
BASE COUNT          962 a         1700 c         1553 g          906 t
ORIGIN      
        1 ctacctgtgg gccggctggc tttacaggcg gcggcggagg aggaggcggg agtagcgttg
       61 gcgcggggcg ggtcggcggc gcgggcgggg gcggggtggc caccggttcc tgtaccggct
      121 cagccggaac ctctgccggg ggcgccggcg agatctccgg ttgtggctct ggggcaggag
      181 gcggaggctc aggcgcagct tcctctgccg cgggcacttc cggttggggt ggcttttcgg
      241 cctctggcgc ctctggaacc gtttccggaa actcaggcgc ttggctttcg ctgggctccg
      301 caacagctac cgccgcttcg ccattctcgg cggtcggctc agcagccttc ttgcgcgagc
      361 ggcggacctt gcttacgtca acgcggtccc ccacctcgat cgaggtcaca tcctggccgg
      421 ccgagaacca ctcgcggatt gattcggcca agcctgcgga gatcgcagcc atgtggttct
      481 tcagctccac accctcggcg cggcacttat ctatgacctc cttgctggta acgcccagct
      541 ctttggccaa ggtgtgaact ctcagggtct tcgccacgca gatctccgtc tgtccggtaa
      601 ctgccggaac gcagatgcaa actcgccgcg ctcgggccgc cttttccaac gataccgcca
      661 gggcggcgcg agagtcggta gcaaccaaag gcacaatccc cctcgcggcc ggccggcgat
      721 ctaaggccga ggcctcaagc ggcgtacctt ggactcttgt agtataggat atcctggttt
      781 gctctcttac gccagcttaa gaaggacgtt tcttaacact tttcgcggag ccggcctccc
      841 cagctgccga tagaacaagc gcgtgggccc gacgcggcgg cgagggcgtt aaatcttcct
      901 aagatgggcc ggaaattgac actgacctag tggaccggta aagttattta gcgcggaaca
      961 atgcgttgcg cggctgcgtc gctccggcgg tcgaactcca acctgtaaat cgcggtagta
     1021 gcaggcgggg tacacgaccc gaaaacggct tctcaggcga tcgaggtccg catgaaagtc
     1081 tttatgctgg gctgggaatt cccgccattc atcagcgggg ggctgggtac tgcttgctac
     1141 gggttgacca agggcttgga ccacctgggc accgagatta ttttcgtcct tccccgcggc
     1201 ccagagaaac tccagctcga aacatcgccg accaccagcc aagcagcctc ccagcgacgc
     1261 ccggaagcct tccgctatac ccgctttcgc accgttgacg ccgtgatccg gccctacgtc
     1321 agccccggag tcgaggacga gcccgaaccg gcgacagtcg gcgcacgccg agccaaagtc
     1381 cgggccaagg ggcccgccga acgcgtacgc atcatcgccg ccagctccag cgaaggtccg
     1441 ggaggccatt acggcggcga tttgttttac gaggtcgagc gctatgccgg gctggcctgc
     1501 gagatcgccg ccagcgaagg agacttcgac ctggtccacg cccatgattg gatgacttac
     1561 ccggccggca tcgccgtcgc cgatatgacc gggaaaccgc tggtcgtgca cgttcactcg
     1621 accgagttcg accgctccgg cgtgcacgta aaccagcgca tttatgacat cgagcgcgcc
     1681 ggcatgaccc gcgcccagcg cgtcatctgt gtcagccact tcacgcgtag cgtcgtcctg
     1741 cagcactacg gggtggaccc gcgcaaagtc gacgtcgttt ataacgccgt cgaggaaaat
     1801 ggaacgcctg agtcgcgcac cctgccgccc atcgcgcatg acgagaaaat cgtcctcttt
     1861 ctgggccgca tcacgatgca aaaaggcccg gagtacttcc tggcggccgc caaacgcgtg
     1921 ctggaagtga tgggcaacgt gcgctttatc atggccggca gcggcgacat gacgcgccgc
     1981 atgatcgagc tggccaatca gatgggcatc gggcacaagg tgctcttcac cgggttcctc
     2041 cggggcgatg acgtgcggcg cgttttccga atggccgatc tgtacgtcat gccgtcggtc
     2101 tccgagccct tcggcattgc cccgctcgaa gccctggcca acgacgtacc cgtgctcatc
     2161 tccaagcaga gcggcgtctc tgagattctc agccacgccc tcaaagtcga tttctgggat
     2221 gtcgacgaaa tggcaaataa gatcatcgcc gtcttgagac atccgccact ccaggctacg
     2281 ctgcgcgatc atggcagcat cgaagtgcgg cgtttgagct gggtcgactc cgcccgaagc
     2341 tgcttgaagg tttacgagac cgccgttagc ccggcttaag cagcttagcg cgcgaaatgg
     2401 cctccgtctg tttttacttt caagtacacc agccgttccg cctgcgtcga ttcagtgtct
     2461 tcgaccgcag ttcggattac tttgacgacg agcgcaaccg ccagatctgc cgcaaggtcg
     2521 ccgagaaatg ttacctgccg gccaacgccg tgatcgagga gctggccaca cgctatgccg
     2581 ggcgcttccg cgtggcctac agcctcaccg gctgcgtcat cgatcagttc cggctttatg
     2641 cgccggaggt gcttgaatct tttcagcggc tggcacgaac gggcgcggtc gagtttctct
     2701 cggaaaccta ctaccactcg ctcagcctgc tctattcacg cgaggaattc gcggagcagg
     2761 tcgccctcca ccgccgcaca gtacaggagc tattcggcca gacgccgcgc gtcttccgca
     2821 acaccgagct gatatacagc aacgatttgg cccgcatcgc cgcccaaatg ggctacaacg
     2881 tcgtgctctg cgagggggcc gacaacctgc tcggctatcg ctcgccgact tacctctaca
     2941 ccgcccccga tggcattccc gttcgcctgc tcctgaaaaa ctaccgcctg gcggacgaca
     3001 tcgccttccg cttcggaaac aagaactgga gcgagtggcc cctgaccgcc gacaagttcg
     3061 cccgctgggt cgaccagatc aacggccacg gctacgtcgt caacctgttc atggactacg
     3121 agaccttcgg cgagcaccag tgggccgaaa ccggcatttt cgagttcctc cgccatatgc
     3181 cgtccgagct cttcaagttg ccgggcaacg acttcaagag cccgagcgag tgcgccgcga
     3241 cgtatccggc cagcggcgaa tacgacgtgc cgcacttgat ctcctgggcc gacacggagc
     3301 gcgacctctc ggcttggcta ggcaacgcca tgcagtccaa tgccctgcac gagctgtaca
     3361 agctcgaacc ggcggtgaaa gcctgcggcg acccgaccat cctgcgggat tggcggcggc
     3421 tgcaaagctc ggatcatttt tactacatgt gcaccaagtt cctggccgac ggcacggtgc
     3481 accagtactt caatccgtac gactcgccgt acgattcgta catcaacttc atgaacgtgc
     3541 tggatcacct gcgcagccgg ctgcccaaac aggacgccga gcccgccccg cgtccgaagg
     3601 ccagccgagc aaagtccacc accgcggcgc cgaccgtcaa agccggccgc aagccgcgtt
     3661 cgcaaaaacc caaggccagt tgatcgggga taaggcgcgc gcggccgttc tcgccacctg
     3721 ccaacgggtc ccacatggac gggcgctatc cgttgcactg gcggtccgag ccggcccgat
     3781 cggagcctgc agcgagatcc gagcctgagc ggccagcgag ggcctcctgg tccgagcacc
     3841 ttgcacgaac cgaaccgcca gaacgagccg gggcgtgtct ccagaaatgc agagcagagc
     3901 tgggcgtgtc gtcccggaat aaagtcaaaa gcctccgcac ctgcgcagta ccagttccgg
     3961 gtacacgtgc cgccccgaaa tggccggggg ctggccgtcg cgtgcggctt agctgcgcct
     4021 ggcgcgtaag tcccgaggac aactctagac cgggtcacgg ccttcggaag caccaggcgc
     4081 gctttgctgg tcttccttgt cccacgaacg cagcgctgcc tcaagttccc ccaccaaagc
     4141 agacaggctc gtcttgaccg cccgccagat ggcgtccaca tcgatgttat agtaggcatg
     4201 caccaggatg tggcgcatgc cgacgatctt ccggccaagg caggcgcggc gcccgcctcc
     4261 ggccggcgtc gctgacgcgc gaagccgctt cccccatgtc ctggacacag ttcaccaacg
     4321 cacgcagcag catgcggtcg cggtcgagat cggcgcgcgt ccgtccctgc gtgaagccaa
     4381 tcgcgtctcg ggccgcgcca ccatgtgggc caggcgctcg cggtcgccca gtgggtcagc
     4441 cccgcgttca cgcggcatat tgcatctgac gttcggccag cacgcgccga agaatcggcg
     4501 gcgatagaaa tccgggcgtc ttcaggtcca cttcgcgccc cagcaggctg gtcaactcga
     4561 cctgcatgcc gcccaggtcc agcaggctcg gcgtgccgcc gggcggaaac tcgatcagca
     4621 cgtccacgtc gctgtccggc ccaaaatcgt cccgcaggat cgagccatat agggaaaggc
     4681 gcacgatgcc gtgccttcgg cagaaggcgg cgatctcgtc ggggtcgatc cgcaagccgt
     4741 gtcgttccgc aatcatgagt gctcctggaa gcattttacc gcaggcgaac ccagtcggct
     4801 ttcagcgatt caggcggcat acaacaaccg ggcggagtgc aggacctcgg cgcgaaaata
     4861 gggcgacaaa tccaaaggtg tgcgcagatc aacgggtcgg ccgagtagct cggcgagttc
     4921 gacgagcata ccccccaggt cgaacaggct gacgcgcacg ccgggcagaa attcgaccaa
     4981 tacgtcaatg tcgctggtgg gcgtagtcct ggcgcaggat tgaaccaaac aggctcaggc
     5041 gcgcaacgcc gtgccgccgg caaaaatcag cgattttgac ctcgtcgaac tgaacgccgt
     5101 gcaaggtcat gagtcgctcc t
//
LOCUS       DAOSYS010000255         5115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13593_length_5115_cov_4.753360, whole genome shotgun sequence.
ACCESSION   DAOSYS010000255 DAOSYS010000000
VERSION     DAOSYS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13593_length_5115_cov_4.753360"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..760)
                     /locus_tag="PLU99_13260"
     CDS             complement(374..760)
                     /locus_tag="PLU99_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO18059.1"
                     /translation="MQNGKHVILCIDDDEDILFALRMVLEKNGYGVLTARSAEEALKV
                     YKETRPDLIIVDLMMEEVDAGTSFVQSIRAMGSPPPIYMLSSVGDNLNVSADYAELGL
                     EGVFQKPLDNDTLLAILRAKLKPAKK"
     gene            complement(760..1323)
                     /locus_tag="PLU99_13265"
     CDS             complement(760..1323)
                     /locus_tag="PLU99_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOO18060.1"
                     /translation="MAERVLVLGYGNPGRLDDGLGPALAAALEKDNLPGVTVDSDYQL
                     TVEDADSAARHDVVIFADAALAGSEPFYFQRVQPRQEELGFSSHSLEPAGVLTLARKL
                     FNARPQGFALGIRGYRFNEFGEELSPHAQANLEAALEFVRQALRTGRFEEVGHPPQAD
                     QVDRDFVTKSAGSAPAPQPGLSKAGEN"
     gene            complement(1323..2780)
                     /locus_tag="PLU99_13270"
     CDS             complement(1323..2780)
                     /locus_tag="PLU99_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOO18061.1"
                     /translation="MVTATATNGLKRVVIEPVTRVEGHGKVTILLDHQNKVKQARLHI
                     VEFRGFERFIQGRPFWEVPVLVQRLCGICPVSHHLAAAKAMDRIVGGEKLTPTAEKVR
                     RLMHYGQFFQSHALHFFHLVSPDLLFGFDADPAIRNVVGVAAKHPDLAVQGVMMRKYG
                     QEIIRITAGKKIHGTGAIPGGVNKNVSIAERDFLLKDFEQMLTWARAALKLCKDYTLE
                     HLEKLAPFGSFDSNHLSLIRTDGAMDIYHGNLRAIDKDGKKIFDQVDYQNYLQYIAEE
                     VRSWSYMKFPFIKSLGPEAGWYRVGPLARVNTADFIDTPEAEAARKEFMALTKGKPNN
                     LTMAYHWARLIELLHSIEKIGTLLHDKDLQGTDLVTKGQRRGEAVGLIEAPRGTLFHH
                     YRVNENDQITMANLIVSTTSNNEPMNRAVQKVCEDHLSGKEITEGLLNHVEVAIRAYD
                     PCLSCATHALGQMPLVVELIDPEGNVLDRRSRGQM"
     gene            complement(2913..3458)
                     /locus_tag="PLU99_13275"
     CDS             complement(2913..3458)
                     /locus_tag="PLU99_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOO18062.1"
                     /translation="MAKPKVATTSLAGCFGCHMSLLDIDDRILKLVELVDFDKSPVDD
                     IKEFTGRCAVGLIEGGCCNEENVRVLKDFREHCDILISVGDCAIMGGIPAMRNMVPLK
                     ECLEEAYLNGPTVHNPRGKIPDDPEIPLLLNKVYPCHEVVKIDYHLPGCPPSADTLWQ
                     ALVALLTNKPLELPYELIKYD"
     gene            complement(3464..4186)
                     /locus_tag="PLU99_13280"
     CDS             complement(3464..4186)
                     /locus_tag="PLU99_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOO18063.1"
                     /translation="MSQEVTFTIDGIEVKAAPGQTILEAAEKAKIYIPRLCHQKDLAP
                     FGSCRICTVLVNGRPQAACTQPAAAGMVVENECEKVRRYRKNLIEMLFVEGNHFCMFC
                     EKSGKCELQALAYRYGITAPKYPYMWPKRDVDASHKDVFIDRNRCVLCGRCVRASRDL
                     DGKNVFQFVERGERRRVAVNAEARLSDTDASVTDKAVEMCPVGAILKKRVGYNVPVGQ
                     RPYDHKPIGSEIENGQSAESKR"
     gene            complement(4201..>5115)
                     /locus_tag="PLU99_13285"
     CDS             complement(4201..>5115)
                     /locus_tag="PLU99_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOO18064.1"
                     /translation="FLEDALAKRRAEGLLGKNVCGKPGFNFDIRIQMGAGAYVCGEET
                     ALISSCEGQRGDPKNRPPFPAQKGYLGCPTVVNNVETLCCVTKILEEGPATFCEYGTQ
                     GSSGTKLLSISGDCTRPGVYEVPFGLTVTEMLKMAGGEDAIAVQMGGASGQMVPPKDF
                     GRKICYDDLATGGSVMIFGPGRDLLKVASRFLEFFIEESCGTCTPCRAGNVLLKERLD
                     RIIAGKGEPADLPYLEELGETIKATSRCGLGQTSPNPVLTTLKNFRPLYEALVKERPV
                     GLRPEFDLQAAVSAAEAIVGRKSVHVEA"
BASE COUNT          885 a         1575 c         1606 g         1049 t
ORIGIN      
        1 tgcttgcgta agggacctga atcgacgacg cgctgctcgc ccccgcgatc gtctatgccc
       61 catgaaatta agtttagcgc ggctcctgaa caatggctca gcggtgggaa atttaagctt
      121 ttctgtgaat taaggtggtc gttaaggtgt tcgcgccggt acggaggggc atgccgcgct
      181 tacagcttgg gcatgccacc cgacttgggg catggcaccc ggcttttctg atattacggt
      241 gcttgggctg taaggagcgc gggtccgatt ttcggaattc agatgccgcc cgcggcgggg
      301 gcggcccgcg cgggcggcat gtggttttct tctggcggcc agcgggctgg cggtggttac
      361 ttttgcgggc tggttacttt ttcgccggct tgagcttggc ccgcaggatg gccagcaggg
      421 tgtcgttgtc gagcggcttc tgaaagacgc cttccaagcc cagttcggcg taatcggcgc
      481 tgacgttcag attgtcgccg accgagctga gcatgtagat cggcggcgga ctgcccatgg
      541 cccgaatgct ctgcacaaag gaggtcccgg cgtcgacctc ctccatcatc aaatcgacaa
      601 tgatcaggtc ggggcgcgtc tccttgtaga ccttgagcgc ctcttccgcg ctgcgggccg
      661 tcagcacgcc gtagccgttc ttttcgagca ccatccgcag ggcgaacagg atgtcctcgt
      721 cgtcgtcgat acagagaatc acgtgcttgc cgttttgcat cagttttccc cagcttttga
      781 caaaccaggt tgtggcgccg gcgcggagcc ggcggacttt gtaacgaaat cacgatccac
      841 ttggtcagcc tgcggcgggt ggccgacttc ttcaaacctg ccggtgcgca gagcctggcg
      901 gacaaactcc agcgctgctt ccaggttggc ctgcgcatgg ggggacagct cctcgccgaa
      961 ttcgttgaag cgatatccgc gaatgcccaa ggcgaagccc tgcggccgcg cattgaaaag
     1021 cttgcgggcc agggtcagca cgccggccgg ttcgaggctg tggctgctga agcccaactc
     1081 ctcctgccgc ggctggaccc gctggaagta gaagggctcc gacccggcca aggcggcgtc
     1141 ggcgaatatc acaacgtcgt gccgcgcggc ggaatccgca tcctcgacgg ttagctggta
     1201 atccgaatcc accgtcacgc ccggcaggtt gtccttctca agcgcggcag ccaaggccgg
     1261 cccgagtccg tcatcaagac gaccggggtt gccgtaaccc agaactaaaa cgcgctcagc
     1321 cactacattt ggcctcggct gcgcctatcg agcacatttc cttcgggatc gatcagttcc
     1381 acgaccagcg gcatctgccc cagggcgtgc gtagcgcacg agaggcacgg gtcatacgcc
     1441 cggatggcga cttccacgtg gttcagcaag ccctcggtga tttccttgcc ggacaggtgg
     1501 tcctcgcaga ccttctgcac ggcccggttc ataggctcgt tgttgctggt ggtcgaaacg
     1561 atcaggtttg ccatcgtaat ctggtcgttc tcgttcacgc ggtagtggtg gaacagcgtg
     1621 ccgcgcgggg cctcgatcag gccgacagct tcgccgcggc gctggccctt ggtcaccagg
     1681 tcggtgccct gcagatcctt gtcatgcagc agcgtgccga tcttctcgat gctgtgcagc
     1741 agctcgatca gccgcgccca gtggtacgcc atggtcaggt tgttgggctt gcccttggtg
     1801 agggccatga attccttgcg ggccgcctcg gcctcgggag tatcgatgaa gtcggccgtg
     1861 tttacccgcg ccagcggacc gacgcggtac cagccggcct ccggcccgag cgacttgatg
     1921 aacgggaact tcatgtacga ccaggaccgc acttcctcgg cgatgtactg caggtagttc
     1981 tggtagtcga cctgatcgaa aattttcttg ccgtccttgt cgatcgcccg caggttgccg
     2041 tgatagatgt ccatggcgcc gtcggtgcgg atgagcgaca ggtggttcga gtcgaaagag
     2101 ccgaacgggg ccagcttctc cagatgctcg agcgtgtagt ctttgcacag cttcagtgcg
     2161 gcgcgcgccc aggtgagcat ttgctcgaaa tccttgagca ggaaatcacg ttcggcgatg
     2221 gagacgttct tgttgacgcc gccggggatc gcgcccgttc catgaatctt tttgccggcg
     2281 gtgatgcgga tgatctcctg gccgtacttg cgcatcatga cgccctgaac cgccaggtcc
     2341 gggtgcttgg ccgcgacgcc gacgacgttg cggatggccg ggtcggcgtc gaagccgaac
     2401 agcaggtccg gcgaaaccag gtggaagaag tgcagggcgt gggactggaa gaactgcccg
     2461 tagtgcatca agcggcggac cttctcggcc gtgggcgtga gcttttcgcc gccgacgatg
     2521 cggtccatgg ccttggccgc agccaggtga tgactgacgg gacagatgcc gcacaggcgc
     2581 tgcacgagca ccgggacttc ccagaagggg cggccctgga tgaagcgctc gaacccgcgg
     2641 aactcgacga tgtgcaggcg ggcctgcttc accttgttct ggtggtcgag cagaatcgta
     2701 accttcccgt gcccttcgac gcgggtgacg ggctcgatga cgacgcgttt caggccattg
     2761 gtagcggtag cggttaccac gaaaagcctc ctgagggcaa agtgcaaagg gccaagttca
     2821 aagtgccaag ttcaaagtga gcggtgacca gccggcatcc ccgcttgccg gcgcggtctt
     2881 cactttgcac tttgtacttt gtacttgtca ctttagtcat acttgatcag ctcgtacggc
     2941 agttccaacg gtttattcgt cagcagcgcc accagggcct gccagagcgt gtctgcggaa
     3001 ggcgggcagc cgggcaggtg atagtcgatc ttgaccacct cgtggcacgg gtagaccttg
     3061 ttcaacagca gcggaatctc gggatcgtcg ggaatcttgc cgcgcggatt atggacggtc
     3121 gggccgttca ggtatgcctc ttcaaggcat tccttcagcg ggaccatgtt gcgcatggcc
     3181 gggatgccgc ccatgatcgc acaatcgccg accgagataa gaatgtcgca gtgctcgcgg
     3241 aagtccttga gcacgcggac gttctcctcg ttgcagcaac cgccctcaat caggcccacc
     3301 gcgcagcggc cggtgaattc cttgatgtcg tcgaccggcg acttatcgaa atcaaccagc
     3361 tcgaccagct ttagtatgcg gtcgtcgatg tcgagcagcg acatgtgaca gccgaagcag
     3421 ccggccagcg atgtagtggc aactttgggt ttcgccatgg cttttacctc ttgctctcgg
     3481 cagattgccc gttttcgatc tcggacccga tcggcttatg gtcgtagggc cgctggccca
     3541 ccggcacgtt gtagccgacg cgcttcttga gaatcgcgcc gaccgggcac atctcgaccg
     3601 ccttgtccgt gacgctggcg tccgtgtcgg acagccgcgc ttcggcgttt accgcgacgc
     3661 ggcggcgttc gccgcgctcc acgaactgaa agacgttctt gccgtccagg tcgcgtgaag
     3721 cccgcacgca gcgcccgcac agcacgcaac ggttgcggtc gatgaatacg tctttgtggg
     3781 aggcgtccac gtcgcgcttt ggccacatgt acgggtactt gggagcggtg atgccgtagc
     3841 ggtaggccag ggcctgcaac tcacacttgc cgctcttctc gcagaacatg cagaagtggt
     3901 tgccctcgac gaacagcatc tcgatcaggt tcttgcgata gcggcgaacc ttctcgcact
     3961 cgttctcgac caccatgccc gcggccgccg gctgagtgca agccgcctgg ggccggccgt
     4021 tcaccagcac ggtgcagata cggcaactgc cgaagggcgc caggtccttc tgatggcaca
     4081 atcgcgggat ataaatcttc gccttttcag cagcttcgag gatggtctgc ccgggggcgg
     4141 ccttgacctc gatgccatcg atggtgaagg tgacttcctg actcatataa gttcccttct
     4201 ttacgcttcc acgtggacgg atttgcgacc cacgattgcc tcggccgcac tgaccgccgc
     4261 ctgcaaatca aactcgggcc gtagcccgac cggccgctcc ttcaccaaag cctcatacag
     4321 cgggcggaaa ttcttcaacg tcgtcagcac cgggttcgga gaggtctggc ccaatccgca
     4381 acggctggtg gccttgatcg tctcgcccag ttcctccagg tagggcaggt cggccggctc
     4441 gcctttgccg gcgataatcc ggtcgagccg ctctttcagc agcacgttgc ccgcgcggca
     4501 gggcgtgcag gtgccgcagc tttcttcgat gaagaactcc aggaagcgcg acgcgacttt
     4561 caacagatcg cggcccggcc cgaagatcat gaccgagccg ccggtggcca ggtcgtcgta
     4621 acagatcttg cggccgaaat ccttcggcgg aaccatctgc ccgctggcgc cgcccatctg
     4681 aacggcgatg gcgtcttcgc ctccggccat cttcagcatc tcggttacgg tgaggccgaa
     4741 cgggacttca tagacgcccg ggcgcgtgca atcgcccgag atgctcagca gtttcgtgcc
     4801 tgagctgccc tgcgtgccgt attcgcagaa ggtcgccggg ccctcttcca ggatctttgt
     4861 tacacagcag agcgtctcga cgttattaac cacggttggg cagccgaggt agcccttctg
     4921 ggcggggaac ggcgggcggt tcttaggatc gccgcgctga ccctcgcagg agctgatcag
     4981 ggcggtttcc tcgccgcaca cgtaggcgcc ggcgcccatc tggattcgaa tatcaaagtt
     5041 gaagccgggc ttgccgcaga cgttcttccc gagcaggccc tcagcccgtc gcttggccag
     5101 cgcgtcctcc aggaa
//
LOCUS       DAOSYS010000256         5108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13620_length_5108_cov_4.795369, whole genome shotgun sequence.
ACCESSION   DAOSYS010000256 DAOSYS010000000
VERSION     DAOSYS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13620_length_5108_cov_4.795369"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1198)
                     /locus_tag="PLU99_13290"
     CDS             complement(194..1198)
                     /locus_tag="PLU99_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOO18065.1"
                     /translation="MKVAVIPAIKKPFMFEERPIPEAGPGQIRIKVAACGVCHSDYHI
                     WEGLFNFARLPIVPGHEVAGHVDQVGPGVDWWKVGDRAGMPWIFSTCGHCDACVAGDD
                     PGCPNQLATGVTVDGGYAPYMLAPAAYATRIPEGIDLAEAAPLFCAGLTVYTPLAEAG
                     VRSGMTVAVQGLGGLGHLGVQYARALGARVVGITRGEEKCAFARSLGAEEAIDTQRED
                     PGKALRRLGGADLILTTVTTAAAIAPLLDGLKPRGTLALVGAAPETIPIVPMYVVSRR
                     LRIIGSIIGNRRQMREVLDVAVRHNVRPRIERYRLEQVQEVFQRMAENKTRYRAVLTF
                     "
     gene            1367..1927
                     /locus_tag="PLU99_13295"
     CDS             1367..1927
                     /locus_tag="PLU99_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOO18066.1"
                     /translation="MLGRNPYRDVPAGADPPHVVNTIVEIPKGRRSKFEVDKNTGLMR
                     LDRYLFSSSHYPGDYGFIPQTLAEDGDNLDVLVMVNEPTFSGCLIETHVIGLFRMRDR
                     GVPDFKILGVPHPDPLFNEIRDLDDVPRHFLLEVEHFFMTYKQLEGVKIESDGWEHAR
                     AAEEEVQASIRRYVELIARRAQGLLK"
     gene            complement(2058..2702)
                     /locus_tag="PLU99_13300"
     CDS             complement(2058..2702)
                     /locus_tag="PLU99_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18067.1"
                     /translation="MSDRRVSSNESAAQTARRAGGGRFAHHLLLALLAGSATALTACS
                     RSETAIELDRADSAAGQLLLPAKIEIQRYWTRPVNLEGGPEPDGMDVIVAAFDSCGDN
                     TKMVGVLHIELYTRRPASSDKLGQRLAFWAVELNSIEQMTEHWDSLGRFYRFPLKLAA
                     PPLAPGRYVLTVTLQPPFGDRLMDEYYFEHEAGKVGQKSAPRRGAFLQAGSDGA"
     gene            3251..4036
                     /locus_tag="PLU99_13305"
     CDS             3251..4036
                     /locus_tag="PLU99_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18068.1"
                     /translation="MIRPIDPGENPSVSGEQTSRIEGVERYERPTERVERVERYEAPE
                     RRPDRVSAAVGVAGAGVLAFGGILALALSIIGLAGFYPATLAAIALLCLAGALFLKGC
                     ASAARVPVSEGFAGSTVFSLLDLGLGTTIELVGGAGGIVLGILALVGIIPLKLLAISV
                     IVIAGTLMLATTRPAQGWGDKTREAMRVAVLSTVGVQVFVGAGAIVLGILALNDYYPL
                     TLSLVAFLSMGAAALLVGLAASGQLAQVISRRRHRHDVEPART"
     gene            complement(4153..4821)
                     /locus_tag="PLU99_13310"
     CDS             complement(4153..4821)
                     /locus_tag="PLU99_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOO18069.1"
                     /translation="MKFSRLGLTVVALVFILAGAASWAQPDRTPPTPPLPAPQLSGGM
                     PLAEALATRRSIRQFAPTPLTPPQLGQLCWAAQGITDAQGKRTAPSAGALYPIELFLV
                     TPTGVQHYKPQTHTLEPHLADDLRPDLSRAALSQETIACAPAVFVITAAVGRTAVKYG
                     PRAERYCFLEAGHVAQNILLQATALELCGVPMGAFEDAEVARVLKLPPGFQPLYLVAV
                     GQAK"
     gene            complement(4930..>5108)
                     /locus_tag="PLU99_13315"
     CDS             complement(4930..>5108)
                     /locus_tag="PLU99_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOO18070.1"
                     /translation="VVQWLTYLNPLRYFLVIIRGIFLKGVGLQILWPQMLALTIIGVL
                     TFTLAVRRFHKTLT"
BASE COUNT          926 a         1659 c         1612 g          911 t
ORIGIN      
        1 ggccggcagc catgccagag ccagcaccgc ggcatacgcg gcgaatagcc cgggacgcgc
       61 ggcgcgcaag tgtgccatac cacatgtcaa acgcatgaaa cgcctctcaa cccctgcaag
      121 tttcccgagt tcttgcagac gctgcaagcg gccgccggaa ccggcgactc tgacagggta
      181 cgcgagatcg tgatcagaag gtgagcaccg cgcgatagcg agtcttgttt tccgccatgc
      241 gctgaaagac ctcctgcacc tgctccaggc gatagcgctc gatacgcggg cggacgttgt
      301 gccgcaccgc cacgtcgagg acctcccgca tctgccgtcg attgccgatt atcgacccga
      361 tgatccgcaa tcggcgggaa accacgtaca tgggcacaat cggtatcgtt tcgggtgcgg
      421 cgccaacgag cgccagcgtg ccgcgcggtt tcagcccgtc gagcaaaggc gcgatcgccg
      481 cggccgtggt aaccgtcgtg aggatcaagt ccgcaccgcc caggcgccgc aaggccttgc
      541 ccgggtcctc gcgctgcgtg tcaatcgcct cttctgcccc gagcgatcgg gcgaaggcgc
      601 atttctcctc gccgcgcgtg atgccaacga cccgcgcccc caaagcccgc gcatattgca
      661 cgcccaggtg ccccaggccg cccaggccct gcacggccac cgtcatgccc gaccgcacgc
      721 cggcttccgc cagcggcgtg tagaccgtca ggcccgcaca aaacagcggc gcggcctcgg
      781 ccaggtcgat gccctccggg attcgcgtgg cgtaggcggc cggcgcgagc atgtacggcg
      841 catagccgcc gtccacggtg acacctgtgg ccaactgatt cgggcaaccg ggatcgtcgc
      901 cggccacgca cgcgtcgcaa tgtccgcacg tcgagaagat ccacggcatg cccgcgcggt
      961 cgccgacttt ccaccaatcg acgccggggc cgacctggtc cacatgtccg gccacctcgt
     1021 gcccgggcac aatgggcagg cgggcaaaat tgaaaaggcc ctcccaaatg tggtagtcgc
     1081 tgtggcagac gccgcaagcg gccaccttga tgcgaatctg ccccggtccc gcctcgggga
     1141 tcgggcgttc ctcaaacata aatggctttt tgatggcggg aatgacggcg actttcatgt
     1201 tttccctcgg caagcaggag ttttgaaaac gtaccgcgct tcgaagtgcg tattatagcc
     1261 cggtccggca cgcgaccggc ggctcgccac gagttcgcgc cggcttggac atttccggcg
     1321 ttgctcatac tcttatctaa ggtagaacaa aacccggagc cggaccatgc ttggacgcaa
     1381 cccgtatcga gacgtacccg caggcgctga tccgccgcac gtcgtcaaca ctatcgtcga
     1441 aatccccaaa ggccgccgca gcaagtttga ggtggacaag aacaccggcc tgatgcggct
     1501 ggaccgctac ctcttcagct ccagccacta tccaggcgac tacggcttca ttccgcagac
     1561 gctggccgaa gacggcgaca acctggacgt gctggtcatg gtgaatgagc cgaccttcag
     1621 cggctgcctg atcgagacgc acgtgatcgg gctgttccgc atgcgcgacc gcggcgttcc
     1681 cgatttcaaa atcctaggtg tgccccaccc cgacccgctg ttcaacgaga tccgcgacct
     1741 agacgacgtg ccgcgccatt ttttgctcga ggtcgagcac ttcttcatga cgtacaagca
     1801 gctcgaaggc gtgaagatcg agtccgacgg ctgggaacac gcccgggcgg ccgaagagga
     1861 agtgcaggca tcgatccggc gctacgttga gctgatcgcc cggcgggcgc agggcctgct
     1921 taagtagcac acggcctgct tagatagcac gttggaaccg atccgcggag gggcgtggcc
     1981 tcgatgcgcg cggccggttc gcaccggttc accgacacgc ccacacactc atccacatga
     2041 acccccctgc tcgccgatca cgccccgtct gagccggcct gcagaaacgc acctcgccga
     2101 ggggcggact tctgccccac cttgcccgct tcgtgctcga agtagtattc atccataagc
     2161 cggtcgccaa atggcggctg gagcgtgacg gtcagcacat agcgccccgg cgccagcggc
     2221 ggcgcggcga gcttcagcgg aaaacggtag aaacggccca gcgagtccca gtgctcggtc
     2281 atctgttcga tcgaattgag ctcgaccgcc cagaacgcca accgctgccc gagcttgtcg
     2341 ctgctggccg ggcgccgcgt gtacaactcg atgtgcagca cgccgaccat tttggtgttg
     2401 tcgccgcagg aatcgaacgc cgccacgatc acgtccatcc catccggctc ggggccgccc
     2461 tccaggttca ccggacgcgt ccagtagcgc tgaatctcga ttttggccgg aagcagaagc
     2521 tgaccagcgg ccgaatcggc gcggtccaat tcgatggccg tttccgagcg agaacacgcg
     2581 gtcagcgcag ttgccgagcc ggccaaaagc gccagcagca ggtggtgcgc gaaccgccct
     2641 cccccggctc gtcgtgcggt ttgcgctgcg gattcgttgg acgacactcg tctgtcgctc
     2701 atcttaaaaa ccagcccaaa aaaagccgcg ccagccccct tataaccgaa cccttgaggg
     2761 ttggctacac tcccatcctt gcggcgcctg actggctgga aaggggcctt gctcagattc
     2821 tccgtttggg agccggctgg cgaggacgcg tggaagccgg ctgagcgagt gcgggttggt
     2881 ttttggccgg cgcgcaatgc ggtcgcagcc cttgccggcg ctttaagctc ggatcattgt
     2941 cattgccttc gccggcgctt cggggctcgg attgcggttc gcgcggccct ctttggcgct
     3001 tcgaaatcga aacattgcgc tcgcttgcgc ttcgggctcg gattgcggcc gagcgcattt
     3061 cgcgcacgca aattcgctaa atgccattac gttcaactca cttcgaattc aggcgagttt
     3121 caaggtccga atgctagact acgcgcagtg agcgagcgag cgagagtgag tatttgattg
     3181 gcacgctcgg gcgttccgga agtggcgagc accgcgtcgg aaacctgttg cgagaacaaa
     3241 aggtgagacc atgattaggc caattgatcc gggtgaaaac ccctccgtat cgggagaaca
     3301 gacatcgcga atcgaggggg tcgagcgcta tgagcgtccg accgagcggg tggagcgtgt
     3361 cgagcgctac gaagccccag aacgtcgccc cgaccgggtc agcgcggccg tcggcgtggc
     3421 cggcgccggc gttttggcat tcggcgggat tctggcgctg gcgctgagca taattgggct
     3481 ggccggcttc tatcccgcca ctctggcggc gatagctctg ctgtgcttgg cgggcgcgct
     3541 gtttctcaaa ggctgcgcaa gtgcggcgcg ggtgccggtt tctgaggggt tcgccggctc
     3601 caccgttttc tctttgctcg acttgggcct gggcacgacg atcgagctag tcggcggcgc
     3661 gggcggcatt gttctgggaa ttctcgcgct ggtgggcatc atccccttga agcttctggc
     3721 catttcggtc atcgtcatcg cgggcacgct gatgctggcc accacgcgcc cggcacaggg
     3781 ttggggcgac aagacgcgcg aggcgatgcg ggtcgccgtg ctctccacgg ttggagtgca
     3841 ggtctttgtc ggcgcgggcg caatcgtgct gggtattctc gcgctcaacg attactaccc
     3901 actgacgctg tcgctagtgg ccttcctgtc catgggcgcg gcagctctgc tggtcggctt
     3961 ggcggccagc ggccaacttg cgcaggtgat ttctcgtcga cggcatcgtc acgacgtgga
     4021 gcccgcgcgg acgtaggact tcattgcggc cctgcgacgg gcaatattga accaggcggc
     4081 aaaccctgcg acggctgagc agtagagtca cgccggcgcg gggtttacgt ctaaatggtc
     4141 actccgcggc ggtcacttag cctgccccac cgccaccagg tagagcggct gaaagccggg
     4201 cggcagtttc aatacccgcg cgacttctgc gtcctcaaac gctcccatcg gcacgccgca
     4261 cagctcgagt gccgtcgcct gcaggagaat gttctgcgcc acgtgtcccg cttccaggaa
     4321 gcaataacgc tcggcccgcg ggccgtattt gactgccgtg cgaccgaccg ccgcagttat
     4381 cacgaaaacg gccggggcgc aggcaatcgt ctcctgtgaa agggcggcgc gggacaagtc
     4441 aggccgtagg tcgtcggcca agtgcggctc cagggtatgg gtttgcggtt tatagtgctg
     4501 cacgcctgtg ggcgtcacga ggaacagctc gatcggatag agcgctccag cggagggcgc
     4561 ggttcgcttg ccttgtgcat cggtaatccc ctgcgcggcc cagcacagtt gcccgagctg
     4621 cggcggcgtc agcggcgtcg gagcgaactg ccgtatcgag cggcgcgtcg ccagcgcttc
     4681 cgccagcggc atgccgccgg aaagctgtgg cgccggcaga gggggcgtcg gcggtgtgcg
     4741 gtccggctgg gcccaggatg cggctcccgc cagaatgaac acaagggcca cgactgtcag
     4801 gccaaggcgg ctgaacttca tatcattcct cctcaaacga acagcccaac gaacattccc
     4861 acgtcacgca actgtttttc tcaaaagggt aattatgacg agatccgtcc tccttctgga
     4921 ggacaattct caggtcagcg tcttgtgaaa gcgccgcacc gccagcgtga acgtcaaaac
     4981 gccgatgatc gtcagcgcca gcatttgcgg ccagaggatc tgcaggccga cgcccttgag
     5041 gaagatgccg cgtatgatga ccagaaagta acgcagcggg ttcaggtacg tcagccactg
     5101 caccacct
//
LOCUS       DAOSYS010000257         5105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13634_length_5105_cov_6.385743, whole genome shotgun sequence.
ACCESSION   DAOSYS010000257 DAOSYS010000000
VERSION     DAOSYS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13634_length_5105_cov_6.385743"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2596)
                     /gene="secD"
                     /locus_tag="PLU99_13320"
     CDS             complement(<1..2596)
                     /gene="secD"
                     /locus_tag="PLU99_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOO18071.1"
                     /translation="MWEKNVGQRVLIILVVLVAAAALIRNPKNTLRPGLDIAGGVSLI
                     YEIDDKGMEGNPNLAEEVKQQLQKRVDPQGVYDLAWRVMGRNRLEVQMPLPPAQAQQL
                     RKDYADALKDLFSAAITRGELENALQQTGAEREAAIQTLARGSEERLARLNQAAAAYD
                     TYTAALEAYRLGPVEAAQTQSAENPPDEVATNEPASAPASAPVKTMEDLQSALRDAED
                     ALEDAVDAFMATNIDADLFQQTLEMDEGSQMRANSIQQIKDKHPELADKIDRVLATYS
                     VWHKNRGYLDGPQDLQRLLRGAGVLEFRILAEPDPSNPTKFDRYRQQLQQRGREPAAG
                     DEYGWFRIDSPINFFDLKSAADVETFDPRTSRYVAEKLGKDFYVLAKLGAEHGLLADN
                     TWRLVNARAEPDPRSGGLAVHFELDVVGGYRFEQLTRANIERPLCILVDNVAYSAPNI
                     QSKISTQGQITSPGGFSYEKASYLVQTMQAGSLPARLKDTPISERTIGSSLGATNRDM
                     AFRAGVIGVCAVALFMSIYYMVGGLIASGAMLMNVVVVLAVMAMLQARLTLPGIAGLV
                     LTVGMSVDANVLIFERMREEKERGSSLRLIVKNGYEKAFSVILDSNLTTILTCVIIYY
                     VGSEEIKGFGLTLGWGIAISLFTSLFVTRTVFAILIKYGWLKEIRMLKLIGVPKIDWY
                     GLRKIFIPVSICVTLLGLVLFLSRGKRDYLDVEFQGGVSAEIDVKQNPDQPLDDRMIR
                     AELAQVGEIIRDDAAKLAEATVEPVTGTPNLFRLRAPGIPNNRLAALVTEPLEDQGWL
                     MRGGVDDHTEENAVLLRASGTVSAEEFTTFVRGLSATTRNSGADIASSSVNLVTEAGF
                     TTERGRFWN"
     gene            3394..4026
                     /locus_tag="PLU99_13325"
     CDS             3394..4026
                     /locus_tag="PLU99_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18072.1"
                     /translation="MANQARRSRNGPSARAWLAGVLNLLVPGLGIIYLGRAWTGLIVG
                     LIFAAFANLALWAVLLIPDDLPDWGPPLALGLAAGAYVGCQVNFVKSSRDRQKREQEA
                     VRRSALAAVGQALECGDFNAAQAALEPVRHLASQDLLVAYRLAQVLTGLGDAQAAWAA
                     WRQVKTLDRHRIYRDEWQTDASEALHSFAAAAQQAPPSAHQCDLRRFLGR"
     gene            4173..>5105
                     /locus_tag="PLU99_13330"
     CDS             4173..>5105
                     /locus_tag="PLU99_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOO18073.1"
                     /translation="MFERFTDRARKVMALANQEAQRFNHEYIGTEHILLGLVKEGSGV
                     GANVLKNLGVDLRKVRLEVEKLVKSGPEMVTMGKLPQTPRAKRVIEYAIEEARNLNHN
                     YVGTEHLLLGLLREQDGVAAQVLMNLGLRLEDVREEVLNLLGAGVESEESAAASTPSD
                     APKKGKSKTPALDSFGRDLTDLAREGKLDPVIGRTDEIERVITVLCRRMKNNPVLLGE
                     AGVGKTAIVEGLAQKIVRHQVPEILHDRRIVVLDLAMMVAGTKYRGQFEERIKAVMNE
                     VRRARNVILFIDELHTLVGAGGAEGAIDASNVLKP"
BASE COUNT          952 a         1678 c         1563 g          912 t
ORIGIN      
        1 cgttccagaa gcgtcccctc tcggttgtga agccggcttc ggtaaccaag ttgaccgatg
       61 agctcgcgat atccgccccg gaattgcgcg tcgtagcgct caagccgcgc acgaacgtgg
      121 tgaactcttc ggcgctgacc gtgccgctgg cccgcagcag cacggcgttc tcttccgtgt
      181 gatcatcgac gccgccccgc atcaaccagc cctgatcctc caacggttcg gtcaccagtg
      241 ccgccaggcg attgttcgga ataccgggcg cccgcagccg gaacagattc ggcgtgccgg
      301 tgaccggctc gaccgtggcc tcggccagct tggcggcatc gtcccttatg atttcgccca
      361 cctgcgccag ctcggcccgg atcatccggt cgtccagcgg ctggtcggga ttctgcttga
      421 cgtcaatctc cgcgctgacg ccgccctgga actcgacgtc caggtaatca cgcttgccgc
      481 gcgacaggaa cagaaccagg cccagcagcg tcacgcagat cgacaccggg atgaagatct
      541 ttctcaaccc gtaccagtca atcttcggca cgccgatcag cttcagcatt ctgatttctt
      601 tcagccagcc gtacttgatc aggatggcga agaccgtgcg cgtcacgaac agcgacgtga
      661 acaagctgat cgcaataccc cagcccagtg tcaggccgaa gcccttgatc tcttcgctgc
      721 ccacatagta gatgatcacg caggtcagga tggtcgtcag gttcgagtcc agaatcaccg
      781 agaaggcctt ctcgtacccg ttcttgacga tcaatcgcag cgaggagccg cgctccttct
      841 cctcccgcat acgttcgaag atcagcacgt tcgcgtccac gctcatgccg accgtcaaaa
      901 ccaaaccggc gattcctggc agcgttaacc gcgcttgcag catcgccatc actgccagca
      961 cgaccacgac gttcatcaac atggccccgg aggcgatcag accgccgacc atgtaataga
     1021 tggacatgaa cagcgctacg gcacatacgc cgatcacgcc ggcccggaag gccatgtcgc
     1081 ggttggtcgc gcccaggctg gagccgatgg tacgttcaga gatcggcgta tccttgagcc
     1141 gcgccggcag cgagccggcc tgcatggtct gcaccaggta cgacgccttc tcatagctga
     1201 agccgcctgg agacgtaatc tgaccttgcg tgctgatctt gctctgaatg ttcggcgcag
     1261 agtaggccac attatcgacc agaatacaga gcggccgctc aatgttggcc cgggttagct
     1321 gctcgaagcg gtagccgccc accacgtcca actcaaagtg aaccgccagg ccgccgctgc
     1381 gcggatcggg ctccgcccgg gcgtttacca ggcgccaagt gttatccgcc agcaggccgt
     1441 gctcagcccc caacttggcc aaaacgtaga agtctttgcc cagtttctcc gcgacataac
     1501 gcgacgtcct tggatcgaaa gtctcgacgt cggccgccga cttcagatcg aagaaattga
     1561 tggggctgtc gattcggaac cagccgtatt cgtcgcccgc ggccggctcg cgcccgcgct
     1621 gctgcaactg ctgccggtaa cggtcgaact tggtcgggtt ggaggggtcc ggttctgcca
     1681 gaatgcggaa ttccagcacg cccgccccgc gcagcagtcg ctgcaaatcc tgcgggccgt
     1741 ccaggtagcc gcggttcttg tgccacacgg aatacgtggc cagcacgcgg tcgatcttgt
     1801 ccgccagctc agggtgcttg tccttaattt gctggatgct gttcgcacgc atctgcgaac
     1861 cctcatccat ttccagcgtc tgctggaaca ggtcggcatc gatattcgtc gccatgaacg
     1921 cgtcgaccgc gtcctctagc gcgtcttccg cgtcccgcaa ggccgactgg agatcctcca
     1981 tcgtcttgac cggcgcactg gccggggcgc tggccggttc atttgtcgcc acttcgtccg
     2041 gcgggttctc cgccgactgc gtctgggccg cttcaaccgg cccgaggcga taagcttcca
     2101 gcgccgcggt gtacgtgtca taggccgcag ccgcctggtt cagccgcgcc aagcgttcct
     2161 ccgagccgcg cgccaacgtc tgtatggcgg cctctcgctc ggcgcctgtt tgctgcaatg
     2221 cattttcgag ctcgcctcgc gtaatcgctg ccgaaaacag gtccttcagc gcgtccgcgt
     2281 aatccttgcg aagctgctgt gcctgcgccg gcggcagcgg catctggact tcgaggcggt
     2341 tgcggcccat gacgcgccag gccaggtcgt acacgccctg cggatcgacg cgcttctgaa
     2401 gctgttgctt gacttcctcg gcgaggtttg gattgccctc catccccttg tcatcaattt
     2461 cgtaaatcaa cgagacgccg cccgcaatgt ccaaccccgg gcgaagggtg tttttcgggt
     2521 tacgaatcag cgcggccgca gcgaccagca ccaccaaaat gatcaggacg cgctgaccaa
     2581 cgttcttttc ccacatggaa attcctttac gaactttgaa tggactatgt aggccagtaa
     2641 atcgaaccaa aacccgccgt cgcggggctc tacgtgctgc cttccccatc cgccggcgac
     2701 gattcgccgg gcatcaacac ggaggaccgg ttcgtttcag aacggtgacc aacgcgggat
     2761 caagcaccgg ccgcgctagg aaatcgggcg ggacatcggc gagcaccggc ggtagatacc
     2821 cgtcagctcg ccagggcggt aatagctcgc cccacgcgga atggggaacc ggacccgcat
     2881 tcaacggacc gcaagcgatg gctcccgcaa gcggcccggc agattcggcc tgcatcgccc
     2941 agtccaagtg ccagggccgt cgcgcgggta ccgcgtctgg ccaaggcgcc gtccaggccg
     3001 gcgtctgggg tttggaatgc gcggggtcgg caggaccgga cgcggggtac tcggccgagt
     3061 cgccgcgctg atcatttgcg ggctcggagg ccggcaccgc cggaacggct gaaccggacg
     3121 ccgcgtcgct tgatgaatcg gcgacgggat cgcagttctt cgggcctggc agcagatgcg
     3181 aacaaagccc cccctcggcc gccatcagca gaaccaagcc cgcaccggcg cagatactcc
     3241 agaacctgtc cgcccataag cgcagcattc ggctaatctt accgcgattt cggatacatg
     3301 ccaacagccc gtaaaacgtc gcgcgccttc atggcacgcc cggtgacgcc tgtgcccggc
     3361 tgtgctatgc tgcgcagaat gcctgcggac actatggcga atcaggcacg ccggagccgg
     3421 aatggaccgt ctgcccgcgc ctggctggcg ggcgttctaa atctgcttgt accggggctg
     3481 ggaatcatct acctgggccg cgcctggacc ggcctgatcg tcgggctgat cttcgccgct
     3541 tttgccaacc tcgcgctctg ggcggtattg ctaattccgg acgatctgcc ggattgggga
     3601 ccgccattgg cactcgggct ggccgcggga gcctacgtag gctgccaagt taacttcgta
     3661 aagtcatccc gcgaccgcca aaagcgcgag caggaggcgg ttcgccgcag cgccctggca
     3721 gcagtcgggc aggcgctgga atgcggcgat ttcaacgcgg cccaagccgc actggagcct
     3781 gtccgccatc tggcatcgca agacctgctg gtcgcctacc ggctggccca ggtgctcacc
     3841 ggtctgggcg atgcgcaggc ggcctgggcg gcctggcgac aggtcaaaac tctagaccgc
     3901 catcgtattt atcgggacga atggcagaca gacgcaagcg aggcgctaca ctcttttgct
     3961 gcagccgccc agcaggcgcc gccgtcagcc caccaatgtg atttgcgtcg ttttctcggt
     4021 cgataataac cgagcagcag ccacgcggct tgctggtgtg tgccgcggca tctagaatca
     4081 aacgccgggt gtggtcctgt aaggtgaccg atccggagag gggcacgccg ggtcagcggg
     4141 cgaccctgct gctgacaagg agattcttgc ggatgttcga acgatttacg gaccgcgcgc
     4201 ggaaggtgat ggcgctggcc aaccaggagg cccagcggtt caaccacgaa tacatcggca
     4261 cggagcatat cctgctcggg ctggttaagg agggctcggg cgtcggggcc aacgtgctga
     4321 agaacctggg cgtcgacctg cgtaaggtac gcctcgaggt cgaaaaactc gtcaaaagcg
     4381 gccccgagat ggtcaccatg ggcaagctgc cccaaacgcc gcgggccaag cgcgtcatcg
     4441 aatacgccat cgaggaagcc cgcaacctta accacaacta tgtgggcacc gaacacctgc
     4501 tgctcggcct gctgcgcgag caggacggcg tcgccgccca ggtcctgatg aatctgggcc
     4561 tgcggctcga ggacgtgcgc gaggaagtac tgaacctcct tggtgcgggc gtcgagtccg
     4621 aggaaagcgc cgccgccagc acgcccagcg acgccccgaa aaagggtaag agcaaaaccc
     4681 cggccctcga ttccttcggc cgcgacctga ccgacctggc ccgcgagggc aagctcgacc
     4741 cggtcatcgg gcgtacggac gagatcgaac gcgtgatcac tgtcctgtgc cgccgtatga
     4801 aaaacaaccc ggtgctactg ggcgaggcgg gcgtgggcaa gaccgccatt gtcgaaggcc
     4861 tggcgcagaa gatcgtccgt catcaggtgc ccgaaattct gcacgaccgg cggatcgtgg
     4921 tgctcgacct ggcgatgatg gtcgccggca ccaagtaccg cggccagttc gaagagcgca
     4981 ttaaggcggt catgaacgag gttcgccggg cgcgcaatgt gatcctgttc atcgacgagc
     5041 tgcacacgct ggtcggcgcc ggcggcgcgg aaggcgccat tgacgcttcc aacgtgctga
     5101 agccg
//
LOCUS       DAOSYS010000258         5085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13696_length_5085_cov_5.868390, whole genome shotgun sequence.
ACCESSION   DAOSYS010000258 DAOSYS010000000
VERSION     DAOSYS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13696_length_5085_cov_5.868390"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..577
                     /locus_tag="PLU99_13335"
                     /pseudo
     CDS             99..577
                     /locus_tag="PLU99_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            611..1144
                     /gene="rnpB"
                     /locus_tag="PLU99_13340"
     ncRNA           611..1144
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLU99_13340"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1225..1710
                     /locus_tag="PLU99_13345"
     CDS             1225..1710
                     /locus_tag="PLU99_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18074.1"
                     /translation="MSLLAAGCIGVVLVLLVAVLFGILGVCLLFPGWRASWLPGVLLL
                     GVCAVVAWLAIMAARGVLRWFRKPVAPCDVSPPPLPGICPACGEADLYRAAGSTALPW
                     SIFTARCVSCASRFRVPRRLWIALPEANPRAPHETFEDRVREREGSFDRQLRVAPHLW
                     D"
     gene            complement(1931..2941)
                     /locus_tag="PLU99_13350"
     CDS             complement(1931..2941)
                     /locus_tag="PLU99_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18075.1"
                     /translation="MKHSKWNSTRAMLNALCGNGRVAVVAAILGLAAAAAAQEHVVVN
                     FDKAGLGEGVHAGNIFLSSGVRITSCTAPDAISVGGNITLTAMQPWFELWRYGWAVSA
                     PNYAVALDAGVRDILLSFTVPVTSVEMDLDFYPNEAVDTDRLVVLQSVGSNTFKVLKF
                     VTFTDEKGEPPAKHVSIDMGGDSFSHVLFQTLTELEGFDNLAFDTTGPIPEPEDEGPP
                     GPKPPGSGGWSGGGGGSGNGTIPPPSINDNSSGAPGSNDNKTPESPTTGDDGSVDSGD
                     STTNPDGEAEVNLNSGSDDVLQPTDTAAGGTCGVPAAIILLCGMLLLRLLSQGGPSRG
                     KP"
     gene            complement(3112..>5085)
                     /locus_tag="PLU99_13355"
     CDS             complement(3112..>5085)
                     /locus_tag="PLU99_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18076.1"
                     /translation="WAPYDALVSGWLSGEAFDDLVGLKRWPIPASIEFPNAEREGGLA
                     DARYARLLSAYLAECARHFAERGWLDRAFLRLLAPVPVDASSVERVERTLAIVRQSEV
                     NLPVVAHLPLRSLRGLGWRDSPLIELPDVGIWAPPGMWFEPGAVREERARGREIWMLP
                     DRPPYSGTLAVESLTSDAECLAWQAYRYGIQGLWIEHAADCGTVDDGRGGGRLPAADC
                     LIYSGRPYGVTDRPLGSIRLKRLRRGLQDHACLKLLERRGKPGAGRLLAEQLVPWGGT
                     DACVDNLVSCRPAAWPRDPRMLAMAREVLLRELAADVAPQIVDADLAARRTDWAVLMS
                     QPGRLRAAVTGIRLIPAEQGINARAFCSLLNVSENEVAGTWQVPSPPIGWTPLPMPET
                     RVGPHCQRMAEMDFQLGGLAYNPDGVQPLELQFVADGLGAITAAARLAVAACPLAPRP
                     PIVDGDLSDWALASNNAAGDFRLVRGPEAGPGQSDRPTLGTQAFFCMDRERLYVAVRC
                     QLKAGEPPICRAENFVTVDGGVPWGQDLVEILIDPRRVPEGTSADLYALQVKPNGVLV
                     ATKGCHTEPQVGQCETWLSGAQVAVQVRHEAWVVELSLPLAALGARATDNTLWGFNVT
                     RLEARRGEYSSWSGARGSCYRPQALGNLLLLAR"
BASE COUNT          879 a         1745 c         1518 g          943 t
ORIGIN      
        1 cggtggcctg gttcggggtc attctggcgg gccttgtggc ccagctttac acgcccgatg
       61 acagcatgac cgggatttgg agctcgatca gccccgcgat ggcggttgtg tttgtcctga
      121 cgctttccat tgtgcccggc ctggtcgagg agctcttttt ccggggccac attcaacgcc
      181 gcctcctgct gcgctggcct cccgcctgcg ccatcgcggt gcagccgtgc tgtctgcggt
      241 cgctcacctg gacgtgattc aatcggtttt cgcgtttccg ctcggcatct ggctcggaat
      301 aattgcgtgg cgcaccggtt ccatctggcc gggagcgatc tgccacgcga tcataaatgc
      361 cgtttgggat attctgaaca tcgtcggcgc ccaatccggc tttcaggtgg agggcgggcc
      421 aatgctgctc agcatatgcg cgattgggct ggcctgcgtc ctggcgtttg tagcctcaat
      481 cgtactgctg gtacggatcg gcccgccgcc gcgaccggat gcacaactcg ccgggggcgt
      541 ggcagaagtg gcgccggaac ccgattacag catttagccg cggcggcttt ggcggtagaa
      601 tctgtatggc agagtgggcc aggcggccgc cggagccgtc ggcacgcaca atccgttgtg
      661 catgtcttgg cgccggagga aagtccgaac tgcacagggc acggtgatgg ctaacggcca
      721 ccgggcgtga gcccagggaa agtgccacag aaaaaacacc gccgacggag gacgtggcaa
      781 ggtgggaatg tgggaaagtg ggaaagtggg aaagtaagag gcgactcgcc tgaacagcga
      841 ccttcacacg ttcccacgtt tccaccttcc cacgccctcc gggtaagggt gaaatggtgc
      901 ggtaagagcg caccgcgtcg ctggcgacag cggcggcacg gcaaacccca ccggcagcaa
      961 gcccaaatag ggggcgatga gccggcccgg ctcgttatcc agccccgggt agggcgcttg
     1021 agcccgtcgg caacggcggg cccagatgaa tggccgctac cagcgccggc aggcccatac
     1081 acggctcgcc cgtgaccacc agccggcgcc ggcacagaat tcggcttacc ggctcactct
     1141 gccgattttt tggatgagcc gcccgcaatg aaagactcgc ataggacgga tggaataggc
     1201 agattgctcg ctcggtgcgg gcgggtgtcg ctgcttgccg cgggctgcat tggcgtagtg
     1261 ctggtcctgc ttgtggcggt cctgttcggc atcctggggg tctgcctgct atttccaggc
     1321 tggcgagcct cctggctacc cggcgtgctg ctgctcggcg tgtgcgccgt tgtcgcctgg
     1381 ttggcgataa tggcggcgcg cggcgtgctc cgatggttcc gaaagcccgt cgcgccctgc
     1441 gacgtcagcc ccccgccact acccggcatc tgcccggctt gcggcgaagc cgacctctac
     1501 cgggcagccg ggagtacggc gctgccctgg agcattttca cggcgcgatg cgtaagctgt
     1561 gccagccggt tccgcgttcc ccggcgactg tggatcgcgc tgccggaggc gaatccccgc
     1621 gcaccgcacg agacttttga agatcgcgtg cgtgaacgag aggggagttt cgaccgccaa
     1681 ctgcgcgttg cgccgcattt gtgggactga tgcttgccgc tgctgcgttg tctggcgtga
     1741 ccgctgcttc acttgcgcaa gtctggccgc tgcccggcgc aaatcggttt gggattgcgc
     1801 ttcttgatcg cccccatgcc ccacgcagaa gaacgcttga gccgaggtgc ccccgtcttg
     1861 tgccaaagcg aggcggggtc ggcgattcgc ctgccggcgc ccaccgaccc cgctctggtc
     1921 ccgttcaggg ctacggtttt ccgcggcttg gcccgccttg actcagcagc cgcagcagca
     1981 acatcccgca aagcaagatg atcgccgcgg gcacgccgca cgttccgccg gcagccgtat
     2041 cggtcggctg cagaacatcg tcactgccgg agttgaggtt tacttccgct tcgccgtccg
     2101 ggttcgtagt gctgtcgccc gagtctactg acccgtcgtc accggtggtc ggagattccg
     2161 gagtcttgtt atcgttgctc cccggggccc cggagctgtt gtcgttgatg ctgggcggcg
     2221 gaatggtacc gttaccgctg ccgccaccgc cacctgacca acctccgctg cctggcggct
     2281 tcggacccgg gggaccctca tcctccggct ctggaatcgg accggtcgtg tcaaatgcca
     2341 agttatcgaa gccttccaat tcggtcagtg tttgaaacag cacgtgggag aacgagtcgc
     2401 cccccatatc gatgctcaca tgcttggccg gcggttcgcc tttctcgtcc gtaaacgtta
     2461 cgaacttcag aaccttgaac gtgttagatc cgactgactg cagaaccacc agtcggtcgg
     2521 tatccacagc ctcgttcggg taaaagtcga ggtccatttc caccgacgtg accggcacgg
     2581 tgaacgagag caaaatgtcc cttacgccgg catccaaggc caccgcgtag ttgggggccg
     2641 aaacagccca gccgtagcgc cacagctcga accacggctg catcgcggtc agagtgatgt
     2701 ttccgccgac ggagattgca tccggcgccg tgcagctcgt gatccgcacg ccgctgctca
     2761 gaaagatgtt gccggcatgc actccctcgc ccaaaccggc cttatcgaag ttgaccacga
     2821 cgtgttcctg tgcggccgcc gctgccgcca gcccaagaat cgccgccacc acggctactc
     2881 tgccgttacc gcatagtgcg ttgagcatcg ccctcgtgct attccatttg gaatgcttca
     2941 tttcaagacc ctcatgtttg tgccgcgacg caggtgtctc acagacgaca cacgtctaca
     3001 gcgggttcag acgccattac accttgtgtg accggcacgt ccgctccgtc aatcaagcat
     3061 ataggctccc cctgaagagc atctgaattc cgcctgagct agtggtagcg cttagcgcgc
     3121 cagcagcagg agattgccaa gcgcttgcgg cctataacag cttccgcgtg cgcctgacca
     3181 tgacgaatac tccccgcggc gtgcttccag acgcgtaaca ttgaaccccc acagcgtgtt
     3241 atctgtcgca cgcgccccca gcgccgccag cggcagcgac aactctacca cccacgcttc
     3301 gtggcgcacc tgcacggcca cctgcgcccc gctgagccag gtttcacact ggcccacctg
     3361 cggctcggta tgacagcctt ttgtagcgac cagcacacca ttgggtttaa cttgcagtgc
     3421 gtaaaggtcg gcgctggttc cttccggcac gcggcgcgga tcaatcagaa tttctaccag
     3481 gtcctgcccc cacggtactc cgccgtcaac cgttacaaaa ttttctgccc ggcagatcgg
     3541 cggctcaccc gcttttaact ggcagcgcac cgcgacatac aaccgctcgc gatccatgca
     3601 gaagaatgcc tgtgtgccca gtgtgggccg atccgattgc cccggcccgg cttcaggtcc
     3661 gcgcaccagc cgaaaatccc ccgcggcgtt gttgctggcc aaggcccagt cggacaaatc
     3721 tccatcgacg atgggcggtc tgggcgccag aggacacgcg gccactgcca ggcgtgccgc
     3781 ggccgttatt gcgcccaggc cgtccgccac gaactgcaat tcgagcggtt ggacgccgtc
     3841 cgggttatac gccagcccgc ccaactggaa gtccatttct gccattcgct gacagtgcgg
     3901 cccgacccgg gtctcgggca tgggcagcgg cgtccagccg atcggcggcg aaggcacctg
     3961 ccaagtaccc gcaacttcat tttcgctcac gttcaggagg ctgcagaacg cgcgggcgtt
     4021 aataccttgt tccgccggaa tcagccgtat gccggtgact gcggcgcgaa gacgcccggg
     4081 ttggctcatc agcaccgccc agtcggtgcg ccgcgccgcc aaatccgcat ccacgatttg
     4141 cggcgccaca tcggccgcca gttcccgcag cagtacttcg cgcgccattg ccagcatgcg
     4201 cggatcacgc ggccaggccg ccggtctgca actgaccaga ttgtcaacac atgcatccgt
     4261 cccgccccac ggcaccagtt gctccgccag cagtcgcccc gcgcccggtt ttccgcggcg
     4321 ctccagcaat ttcaggcacg cgtgatcctg caacccgcgc cgcagccgct tcagacgaat
     4381 cgaacccaac ggccgatccg tcactccata cggccgcccg gaatatatca ggcagtccgc
     4441 agccggaagc cggccgccgc cccgcccgtc gtcaactgtg ccgcagtcag ccgcatgctc
     4501 gatccacagt ccttgtattc cgtaccggta tgcctgccac gccagacatt ccgcgtccga
     4561 cgtgagactc tcgaccgcca acgttccgct gtatggcggt cgatccggca gcatccagat
     4621 ttcgcgtccg cgtgcccgct cctcgcggac cgcacccggc tcgaaccaca ttcccggcgg
     4681 cgcccaaatg cccacatcgg gcaactctat aagcggggaa tcgcgccatc ccaacccccg
     4741 cagactgcgc agcggcaggt gcgccaccac cggcagattc acttcacttt ggcgcacaat
     4801 ggccaacgtt cgttccaccc gctctacact acttgcatcg accggaaccg gagccaacaa
     4861 tcgcaggaac gcccggtcca gccagccgcg ctcggcaaag tgccgcgcgc attccgccag
     4921 atatgcagag agcaagcgcg catagcgcgc atctgccaag cccccttcgc gctccgcgtt
     4981 cggaaattcg atcgatgcag gaatcggcca gcgcttcagc ccgaccagat cgtcgaacgc
     5041 ctcgccgctc agccagccgc tcacgagcgc gtcgtacggc gccca
//
LOCUS       DAOSYS010000259         5045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13838_length_5045_cov_4.574749, whole genome shotgun sequence.
ACCESSION   DAOSYS010000259 DAOSYS010000000
VERSION     DAOSYS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13838_length_5045_cov_4.574749"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLU99_13360"
     CDS             <1..1033
                     /locus_tag="PLU99_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18077.1"
                     /translation="VYGDGNPDFEPVVLTFARRTPSGWERQSIGNMRDDSRVALRFRS
                     DGLAVIAFSADGEAPLMYASQTATGGWNLKIADSDQASGYDVSLVLDEKDDPHMCHWE
                     WASGRARQAYTYRKDGLWFSHVLDAVGGCNVMGSGIAVDGQGNVHMAWQIHECASPGA
                     LFYAKLSKAGWSTTRVDYGFSNYSAGCSLVVDRLGRPHILYGTQGELVAGPSELRYAH
                     LDDEGNWVHELVDDDGDCGEINSVTIDADGFLHVAYYAGDGRTQRGVIRYARSAASVA
                     PAPKLGDLNCDGMVNLFDIDPFVLALIDEAGYAAQFPTCDYMAADTNQDGEVNVFDID
                     PFVALLTGN"
     gene            1593..2723
                     /locus_tag="PLU99_13365"
     CDS             1593..2723
                     /locus_tag="PLU99_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18078.1"
                     /translation="MRSSCLAAAAILLAAQLSPGAAGEDWIITPPQTLTVTSGGGPSE
                     YSATISPDDWHELNIPPGAYAGLRISYDWQRVYGNVQPNGTAFSFYDPSLPPLPWNRV
                     GSYVQSSPLVTVSSSDPVHIVLDEPTFWLRSYYCVPTPYPGGQPLGARFNLWFGNSRV
                     VWDNIQIELYEATVTQLSGDSHSDGLWYRPQVDTWSGIDSGRASVLTPYGVQQFTVDE
                     SGVYGIHTHFYPAEEYPEAGIYLFEDVFDVTGPYEHLLAANEQGDYGIGLPDIKSVYL
                     EAGRTYFLVHADVQPGHQFTYNSWIAGPGQAHVVTTKIGDLNCDDVVNVFDIDPFVMA
                     LTDEAAYAAQFPTCDYMAADTNQDGEVNVFDIDPFVALLTGN"
     gene            complement(3034..4287)
                     /locus_tag="PLU99_13370"
     CDS             complement(3034..4287)
                     /locus_tag="PLU99_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18079.1"
                     /translation="MPKSSAIGEEKTTSDFEADLKAVAHRLRSSILGLMASVNQDVSN
                     PQAIARQFGLDKNLTWKISKVVQESDAFAALAHFPGQASVRALLKAFERAGATESSVR
                     AMRQAAADFDHIVRVHSGDRVTLDMMLDNLVAERRHERMEIHRRTLYRGLSATWGVQA
                     RLQLCVNVVAPSDDPDWVDVAWLSGLVELRRLRRDTPWAIASVGKFADDGSPLPVGTL
                     EALDPRFNGPDTAPLLGDFCSKPLPDIRPAPPAGNMVRYQLVQGQVGKLAAVSCIIGL
                     KGRSLVRRTKAENYTCGEHGARLYTPAELLIHDLYVHRDLRYAMSPELAVYSHMPGGL
                     MYPGCEPGQGLLPVGDPVVSLGGQPPDVLTPEFPRYSKMIHYVFERSGWDMHDFAGFR
                     VKLRYPPIPALAVLRYPLPEPGAET"
     gene            complement(4674..>5045)
                     /locus_tag="PLU99_13375"
     CDS             complement(4674..>5045)
                     /locus_tag="PLU99_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOO18080.1"
                     /translation="DRKRVYVTNAVKHFKWEPRGKRRIHSKPTVAQMRACRPWLEAEL
                     AAIKPHVVVCLGATAAQSLLGVTFRITQQRGKLLENTGWAPYVMTTIHPSSILRAPDE
                     RGRQAAYESFVADLKHLPIIK"
BASE COUNT          966 a         1420 c         1641 g         1018 t
ORIGIN      
        1 agtttacggc gatggcaacc cggacttcga acccgtcgtc ttaacatttg cgcggcgcac
       61 gccgtccggc tgggagcgcc agtcgatcgg caatatgcgg gacgactcgc gtgttgcgct
      121 gcggttccgt tcggatggac tggcggtgat agccttcagc gccgatggcg aggcgccact
      181 gatgtacgcg tcgcagaccg cgacgggggg atggaacctg aagattgcag actccgacca
      241 ggcatcggga tacgacgtct cgctggtact ggacgagaag gatgacccgc atatgtgcca
      301 ctgggagtgg gcctcgggca gagcacgcca agcgtacacg taccgcaaag atggactatg
      361 gttcagtcac gtgctggacg cggtcggcgg gtgcaacgtc atgggcagtg gaatcgctgt
      421 ggacggtcag ggcaacgtac acatggcctg gcaaatccat gagtgcgcgt caccgggcgc
      481 gttgttttat gctaagctgt cgaaggccgg ctggagtacg accagagtcg actacggctt
      541 cagcaactac tcggccggat gcagcctggt ggttgatcgc cttggccggc cgcacattct
      601 ctatggtacg cagggcgagt tagtggccgg cccctcggaa ttgcgctatg cgcatctgga
      661 tgacgagggg aattgggtgc acgagttggt ggacgacgac ggggactgcg gcgagatcaa
      721 ctcggtgacg atcgacgccg acggctttct gcacgtggcc tattatgcgg gcgacggccg
      781 cacgcagcgt ggtgtcatcc gctatgcacg gagcgcggcg tcggtagcac cggcgccgaa
      841 actcggtgac ctcaattgcg atgggatggt caacctattt gacatcgacc cgtttgtgct
      901 ggcgctgatc gatgaggccg ggtacgcggc gcagtttccg acctgcgact acatggcggc
      961 ggacaccaat caggacggcg aggtgaacgt tttcgacatc gatccgttcg tggccctgct
     1021 gacgggcaac tgacgcccgc gcttggtggc gttggttggc ggctttgata ccttcgagtg
     1081 gcgaattctc tgtaatttcc aagaacgagg gcgcatcaag agcgagcgcg ggcaacttgc
     1141 cgctagtccc gcgctcgcac acttccagga gcgataagtc gctgcccggg aaggggggca
     1201 gtcgatgcag gtccccggcc aaaagttata agtcgttgtc tttctggcac ctacgatctt
     1261 tttgcgacgg ccgccggtca accattcgcg gagcaatttg cgcagatggc cgcagccgca
     1321 cggcaattct gcaagaaatc cacaaattct tccaaataga aatttgcatg gacgtggttt
     1381 ctgggttata gtaactattg agtgaaggca acttggttct gccacgccgt gcacggcagt
     1441 gaacgaagga gaggagctcg tcagcgggtc acgcccgccg atgcgttccc ggacgtcgga
     1501 atcgtgcggc tcgagcgcgg accaatctat ctctgccttg tttgccgtac gcaggagaaa
     1561 ggcttcaggc acttaacaca ggaggcgagc ccatgaggag ctcatgctta gccgcggctg
     1621 cgattttgct ggccgcacaa ctgtcacccg gcgctgctgg cgaggattgg atcatcactc
     1681 ccccgcagac gctgaccgtc acgagcgggg gcggcccaag cgagtacagc gccaccatca
     1741 gcccagacga ctggcacgag ttgaatattc cgcccggggc gtatgccggg ctgcggattt
     1801 cgtacgattg gcagcgggtg tatggcaacg tgcaacccaa cgggactgcg ttttccttct
     1861 acgaccccag cctgccgccg ctgccctgga acagggttgg aagctacgtg cagtcttcgc
     1921 cgctagtgac cgtctcgagc agcgatcccg tacatattgt tctggacgag cccacgttct
     1981 ggctgcgcag ctattattgc gtgccaacgc cgtatcccgg cggccaaccg ctgggcgcgc
     2041 gctttaacct atggttcggc aatagccgcg tggtgtggga taatattcag atcgagttgt
     2101 acgaggccac ggtcacgcag ctttccggcg atagccattc ggacggactc tggtaccggc
     2161 cgcaggttga cacctggtcg ggaatcgact cggggcgagc aagtgtcctg acgccgtatg
     2221 gcgtgcagca gttcacggtt gatgaatccg gagtatatgg gattcatacg cacttttatc
     2281 cggctgagga gtatcccgag gccgggattt atctgttcga ggatgtcttt gacgtgactg
     2341 gtccgtacga gcacctgctg gccgccaatg agcagggcga ctatggaatc ggactgcccg
     2401 acattaagtc tgtatatctc gaggctgggc gtacctattt cctggtgcac gcggacgttc
     2461 agccaggcca ccagtttacc tacaatagct ggattgccgg gcctgggcag gcgcacgtgg
     2521 tcacgacgaa gatcggcgac ctgaactgcg acgatgtcgt caacgttttc gatatcgacc
     2581 cgtttgtgat ggcgctgacc gatgaggctg cgtacgcggc gcagtttccg acctgcgact
     2641 acatggcggc ggacaccaat caggacggcg aggtgaacgt tttcgacatc gatccgttcg
     2701 tggccctgct gacgggcaac tagcgccgcg gctgcgagtt ttgccgcgtt cggcctgagg
     2761 tagcggggtt ttgagatcaa gacggccttg gcgctcgcgc cagggccgtt ttgtgctaag
     2821 aggccttgtc agaagtccga acgtctggac gggcaaggcc agagctatgc agtccattgc
     2881 cggacagcaa agccgcagcc atgcagtccc attatgagta ttcagtttgc tctgtcataa
     2941 cgatgttaca gagcacaaaa cagaaaacgc gggcgactca atagtcgccc gcgctcctgc
     3001 gtcatccagt agtaggagga gagtgagttt cgtttacgtt tcggctccgg gctctggcag
     3061 cgggtaacgc agaacggcca gagccgggat tggcgggtag cgcaacttga cgcggaaccc
     3121 ggcgaaatcg tgcatatccc agcccgaccg ctcgaaaacg tagtgaatca ttttcgagta
     3181 gcgggggaat tccggcgtga gcacgtccgg cggctggccc ccaagcgata cgaccgggtc
     3241 gccaaccggc agcaagccct gcccgggttc gcagccggga tacatcagcc cgcccggcat
     3301 atggctgtag accgcgagtt ccggcgacat tgcatagcgg aggtcgcgat gcacgtacag
     3361 atcgtgaatc agtaactccg ccggcgtgta cagccgagcg ccgtgttcgc cacaggtata
     3421 gttctcagcc ttggtgcggc gaacgaggct gcggcctttc aagccgatga tgcacgagac
     3481 ggcggccagc ttgccgacct gcccctgcac cagttgatag cgcaccatgt tgcccgcggg
     3541 cggcgcgggg cggatgtccg gcaggggctt ggagcagaag tcgcccagga gcggggcggt
     3601 gtccgggcca ttaaaacgtg gatcgagtgc ctcaagggtg cccacaggca agggtgagcc
     3661 gtcatcggcg aacttgccga cggatgcgat cgcccagggc gtgtcgcgcc gcaggcggcg
     3721 cagctcaacg agtccgctca gccaggcaac gtccacccag tccgggtcgt cgctgggggc
     3781 cacgacgttc acacacaatt gcaatcgggc ctgcaccccc caggtggcgc tgaggccccg
     3841 gtagagggtg cggcggtgga tttccatgcg ttcgtggcgg cgctcggcga ccaggttgtc
     3901 gagcatcata tccagcgtta cgcggtcccc ggagtggaca cgaacgatgt ggtcgaagtc
     3961 ggcggcagcc tggcgcatgg cgcggacgga gctctccgta gcgccagccc gttcgaaggc
     4021 cttgagcagg gctcggacgc tggcctgacc cggaaaatgg gccagggcgg cgaaagcgtc
     4081 gctttcctgt actaccttgg atattttcca ggtcaggttc ttgtcgaggc caaactggcg
     4141 agcgatggcc tgaggattag atacatcctg attcaccgaa gccataagtc cgagaatcga
     4201 cgagcggagg cggtgggcca ccgccttcaa gtcggcttcg aagtcggaag tcgttttctc
     4261 ttcgccaatt gcgctgcttt tgggcactag cgtcgctcca gttgctttgc ctcgcctggg
     4321 ttgagaataa cacaactcgc aaatggtgtc aagaaatcca aaaatttttc caaatagaaa
     4381 cttgcgtaaa attcttcttt gtgctacatt ctaactagag gtttatatag ccgccgagcc
     4441 gtagccctat ggactcgttt gtgtccgcgt tgaccgatgc agcgggggta cgcgacgaag
     4501 tttccggctt gcgaccgcat actggccgac gtcgacgacg gcgacttggt gaacgtgttc
     4561 gatattgacc cgttgtggca ttgctgaccg ggtactgaga acgggtacag cttccgtgac
     4621 gccgcggagg cttataacgt gagcgcgggc gttcctgttc gtccgcgctc gctttatttg
     4681 atgatcggca aatgcttcag gtcggccaca aagctctcgt aggctgcctg gcggccgcgt
     4741 tcatcggggg cacgcagtat cgaggacgga tgaatggtgg tcattacgta gggggcccag
     4801 ccggtgtttt ccaggagctt gccgcgctgc tgcgtgatgc ggaaggtgac gccgagcagc
     4861 gactgcgcgg cggttgcgcc caagcaaaca acgacgtgcg gcttgatggc ggccagttca
     4921 gcctccagcc acggccggca ggcgcgcatt tgcgccacag tgggttttga atgaatgcgg
     4981 cgtttgccgc gcggctccca tttgaagtgt ttgacggcgt tagtgacgta tacgcgtttg
     5041 cggtc
//
LOCUS       DAOSYS010000260         5026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13896_length_5026_cov_4.823778, whole genome shotgun sequence.
ACCESSION   DAOSYS010000260 DAOSYS010000000
VERSION     DAOSYS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13896_length_5026_cov_4.823778"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(281..781)
                     /locus_tag="PLU99_13380"
     CDS             complement(281..781)
                     /locus_tag="PLU99_13380"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOO18081.1"
                     /translation="MNGSSERSSVASSPSSPASSAAPADAPWYSAGIAFSCAQCGNCC
                     SGAPGYVWVTLQEAGQIADVLGLQLSDFTRRYTRRVGSRLSLIELKGGDCVFLVRTPD
                     GLTNCKIHAVRPVQCRTWPFWQSNLSSPKAWERAGRGCPGMNHGRIHPLPTIRTCLSA
                     NADLPL"
     gene            complement(778..999)
                     /locus_tag="PLU99_13385"
     CDS             complement(778..999)
                     /locus_tag="PLU99_13385"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOO18082.1"
                     /translation="MGVFDKFAKRYQRKQEEKKLRDAAVVSDDDVPIPDMPVEQAKAE
                     HVIGRNDPCPCGSGKKYKKCCFLKDGKKA"
     gene            1174..1521
                     /locus_tag="PLU99_13390"
     CDS             1174..1521
                     /locus_tag="PLU99_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18083.1"
                     /translation="MFQLLEMRPLHAPEVEPDVTVPAEYGTDLLFELETDQEPVETTE
                     HIEFDSAGALGLMADGRRRGSQEDEPEDEDFFELEDEEDEEEDEEEFFDDDFEDEDEE
                     DEDLDDFEDEDED"
     gene            1762..2145
                     /locus_tag="PLU99_13395"
     CDS             1762..2145
                     /locus_tag="PLU99_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020474821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18084.1"
                     /translation="MQTHPEEADYSGCEPSQPEQAALCERRSGERRSKFDRRRGPGRR
                     RTDYRRAAEEGCMNEEQSDFIQAIDEYKRVNNRPFPSWTEILEVVLYLGYRRVAPVGE
                     FGLTKPRQTPLPAKARKPGQEPDKE"
     gene            2357..2542
                     /gene="rpmF"
                     /locus_tag="PLU99_13400"
     CDS             2357..2542
                     /gene="rpmF"
                     /locus_tag="PLU99_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010579822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOO18085.1"
                     /translation="MLPVTKKSKMRKRTRRSHHALRAVQLVACRQCGQARRPHLACPN
                     CGYVNAHTSVKLEKEES"
     gene            2548..3558
                     /gene="plsX"
                     /locus_tag="PLU99_13405"
     CDS             2548..3558
                     /gene="plsX"
                     /locus_tag="PLU99_13405"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOO18086.1"
                     /translation="MRIALDAMGGDHAPREIIRGAIRGLERLGADDQLVLFGLREPLE
                     QECRALELSDPRVEFVYCAETIGMHDTPVEALRQKRDSSIVRMAVAGAHGEVDALISA
                     GNTGAFAAACQLKLGPVEGVSRPGIAVVMPTFYGPVLICDVGANVAPKPHHLHEYARM
                     CTCYARELLHIAQPRVGLVSIGEEEVKGNHLVKEARMLIKQDPLLNFVGNMEGRDIFS
                     GHCDVFICDGFVGNVVLKLTEGLAEGLFQTIIHEIEQESAELARNFQPIVDRIWRRHD
                     FTEYGGAPLLGLNGVAIICHGRSDQRAIANAIRVAQEQVKVGLPAMIASQFVQPRPQG
                     AK"
     gene            3555..4547
                     /locus_tag="PLU99_13410"
     CDS             3555..4547
                     /locus_tag="PLU99_13410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOO18087.1"
                     /translation="MKVDWEVEVAGTGFSVPERIVTNAEFASRIDTSDEWIVQRTGIR
                     ERRFAAPDESTLTFAAAAGRTALAQAQMSPTELDLIVCGTITPDHTLPSTASLLQAEL
                     GCRWIPAFDLAAACSGMVWSFIVAAQYIATGTARNVLVVGAETLTRITDQEDRGTAVL
                     FGDGAGAVILRPSTAPQRRILAMRQGADGTRAMLINVPAGGAKNPASPQTLAERMHYM
                     RMRGREIYKFAVTQMCDIIHETAADAGLRVEDVALIIPHQSNLRIIESACDKAGVAPE
                     RVLVNIDRYGNTSAASVGIALHEARMAGRIKPGDVVMLVAFGAGLTWGSILLRM"
     gene            4549..>5026
                     /locus_tag="PLU99_13415"
     CDS             4549..>5026
                     /locus_tag="PLU99_13415"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOO18088.1"
                     /translation="MTASAAIFPGQGAQEVGMGKDAAEVCAVAADTFAQADEILGFGL
                     SQLCFEGPAERLNATDIQQPAIFTTSVALYRAALDRKKIRPDQFAAMGGLSLGEYTAL
                     HLAGSVPFDAALRLVQRRGQLMQQAAQRSPGGMVSIMGLTEEQVLAVCERVAESGRV"
BASE COUNT          957 a         1513 c         1686 g          870 t
ORIGIN      
        1 cgaagatcca cggcgcgcac agcccggacg agcggactta tccggcctcg gtacagaagt
       61 cgtaccaata cctgaaggcg gttctggcag agctggcgcg cgcgtagctc gactttcgag
      121 acacgcccct gccctgcaca ttcccacatg cacacgttcc cactttcaca cgatcggacg
      181 cgtgggaaag tgggaacgtg ggaacgtggg aacgtgggaa ggtgggaagg tgggaaggtg
      241 ggaaggtggg aaggtacggc gcgtgatgga acgagcgttc tcagagcggc agatcagcgt
      301 ttgcgctaag gcaggtccgg atagtgggca gcggatgaat ccggccgtga ttcatgcccg
      361 ggcagccacg gcctgcgcgt tcccaagctt tgggcgaact cagattggat tgccagaacg
      421 gccaggttcg gcattggacg gggcggacgg catggatttt acagtttgta aggccgtccg
      481 gcgtgcgcac taggaaaacg caatcaccgc ccttgagttc aatcaggctt aggcggcttc
      541 cgacgcgccg cgtgtaccgg cgggtgaagt cggagagctg aagacccaag acgtccgcga
      601 tctgaccggc ttcttgaagc gttacccaaa cgtagccagg ggctccgcta cagcagttcc
      661 cacattgagc gcaagagaac gcgatgccgg cgctgtacca gggggcgtca gccggggccg
      721 cggaggaagc aggcgaagac ggtgaagagg ccaccgagga gcgttctgac gagccgttca
      781 tgccttcttg ccgtctttca ggaagcagca ttttttgtat ttttttcccg agccgcaggg
      841 gcaagggtcg tttcgtccta taacgtgttc ggccttagcc tgctcgacgg gcatgtcggg
      901 aatcggaaca tcgtcgtccg acacgaccgc cgcgtcgcgc agcttttttt cctcctgttt
      961 tcgctgatat ctcttcgcga atttatcgaa cacgcccatc gaccgaccct ttacgaaacc
     1021 ggttctgtca gtatagctaa ttggcggcgc ctatgctaat tgccgcggct gattagcttg
     1081 ctgcgagatt ccgaaaaagt cgcttgacga atctcccgac cggaagtatt aagccgcgcg
     1141 ttgagcctgg aaatcatcgg aaggagcgca cgtatgttcc agttattgga gatgcggccg
     1201 ttgcacgccc ccgaggtgga accggacgtt acggttcccg ccgaatacgg gacggacctt
     1261 ctgttcgagc tagaaaccga ccaggagcca gtggaaacaa cggagcatat cgagttcgac
     1321 agcgccggcg cgctaggcct gatggccgat gggcgccgcc gcggtagcca ggaagatgag
     1381 ccggaggacg aagacttctt cgagctcgag gacgaggagg acgaggaaga agacgaagaa
     1441 gagttcttcg acgacgactt tgaagacgag gacgaggaag acgaagacct cgacgacttc
     1501 gaggacgaag acgaggatta ggacctccgc ttcaattcag gtgcaaatcg ggccggctgt
     1561 gactgctggg cagtcgcggc caggcaattg accgttacga gcttagcaca gagttgtcgc
     1621 aatcacgcca gcgtcgcgtg gccagggagg ccacgcgacg ctggaagcga tttggggggt
     1681 gcgcgcggcg gtcaacgcga gctgcggccg ggcgggcgcg ctgcgctcag ggtcggcggc
     1741 agcggcgccg ataatgtgaa tatgcagact catcctgaag aagcggacta ttcgggctgc
     1801 gaaccgtcgc agccggaaca agcagccctc tgtgaacggc gttcgggcga gcggcggtcc
     1861 aaatttgacc ggcgccgcgg tccggggcgc cgccgcacgg actaccgccg ggcggctgaa
     1921 gaagggtgca tgaacgagga gcagtccgac ttcatccagg ccatcgacga atacaagcgc
     1981 gtcaataacc ggccgttccc gagttggacc gagattctcg aggtagtgct gtacctcggt
     2041 taccggcggg tcgctccggt gggcgagttc ggtctgacca agccgcgcca gacgccgttg
     2101 ccagcaaagg cccggaagcc cggtcaggag ccagacaagg aataggccgg ccgactctcg
     2161 atccctcgat cccccgatcc gttgatccct cgaatcctgg tccctaaacc gccgagatcc
     2221 ctcaatccgc cgaccctgct accttgacac gcgcgggcgt tctgcgtact gtcatccgaa
     2281 ttgtggcttg atccagggcc gtgcacagcc ggcatttgcc ggccggggtg gttatccggc
     2341 ttccgcagga gtagcaatgc ttccggtaac gaagaaatca aagatgcgca aacgcactcg
     2401 ccgttcacac cacgcgttgc gtgcggttca gttggtggcc tgccgacagt gtgggcaggc
     2461 gcggcggccg cacctggcgt gccccaattg cggatacgtc aacgctcata cctccgtcaa
     2521 gctcgagaaa gaggagtcat aaagaccatg cggatcgcgc tggacgccat ggggggcgac
     2581 cacgcgccgc gggagatcat ccggggcgcg attcgcgggc tggagcggct aggagcggat
     2641 gaccagctcg ttctgtttgg cctacgcgag ccgctggagc aggaatgccg ggccctcgag
     2701 ctgagcgacc cgcgcgtcga gtttgtctac tgcgctgaga cgatcggcat gcacgacacg
     2761 ccggtcgagg cgctgcgcca gaagcgtgat tcttcaatcg tgcgcatggc ggtcgccggg
     2821 gcccacggcg aggtggatgc gctcatctcc gcgggcaaca ccggcgcttt cgcagccgct
     2881 tgccagctca aattgggccc cgtggagggc gtttcgcggc cggggattgc cgtggtcatg
     2941 cccacgtttt acggcccggt gctgatctgt gacgtcggcg cgaacgtcgc cccgaagccg
     3001 caccatcttc acgaatacgc gcgcatgtgc acctgctatg cacgcgagtt gctgcacatt
     3061 gcgcagccgc gggtcgggtt ggtttccatc ggcgaagaag aggtgaaggg caaccacttg
     3121 gtgaaagaag cccgcatgct gatcaagcag gatccgctgc tgaacttcgt cggcaacatg
     3181 gaaggccgcg acatattcag cggtcattgc gacgtcttca tctgcgacgg attcgtgggc
     3241 aacgtggtgc tgaagctgac cgaggggctg gccgaagggc tgttccaaac gatcattcac
     3301 gaaatcgagc aggaaagcgc cgagctggcc cgcaatttcc agcccatcgt cgatcgcatc
     3361 tggcggcggc acgacttcac cgagtatggc ggcgcccccc tgctgggcct caatggcgtc
     3421 gccatcatct gccacggccg cagcgaccag cgggccattg ccaacgccat ccgcgtggcc
     3481 caggagcagg ttaaggtggg gcttcccgcc atgattgcgt cccagttcgt acagccgcgg
     3541 ccacagggtg ccaaatgaaa gtggattggg aggtggaggt tgccggcacg gggttcagcg
     3601 ttcccgagcg tatcgtcacg aacgcggaat tcgccagccg catcgatacg agcgacgagt
     3661 ggatcgttca gcggaccggg atccgcgagc gccgtttcgc cgcgcccgac gaaagcacgc
     3721 tgacgtttgc cgccgccgcg gggcgcacgg ccctggcgca ggcccaaatg agccccaccg
     3781 agctggactt gatcgtctgc gggacgatca cgccggacca cacgttgcct tcgaccgcca
     3841 gccttttgca ggcggagctg ggctgccgct ggatcccggc attcgacctg gccgcggctt
     3901 gcagcggcat ggtctggtcc ttcatcgtag cggcgcagta catcgcgacc ggcacggcgc
     3961 gcaatgtcct ggtggtcggc gctgaaacgc tcacgcgcat cactgaccag gaagaccgcg
     4021 gcaccgccgt cctcttcggc gacggcgccg gggcggtcat cctccgcccc tccaccgcgc
     4081 cgcagcgccg catcctggcc atgcgccagg gcgccgacgg cacacgggct atgctcatca
     4141 acgttccggc cggcggggcc aagaatccag ccagccccca gaccctggcg gaacgcatgc
     4201 actacatgcg catgcgcggc cgcgagatat ataaattcgc ggtgacgcag atgtgcgaca
     4261 tcatccatga gaccgcggcg gatgcaggcc tgcgcgtcga ggatgtggcg ctgattattc
     4321 cgcatcaatc caacctgcga atcattgaat cggcctgcga caaggccggc gtggcgcccg
     4381 agcgcgtgct ggtcaacatt gaccgttacg gcaacacctc ggccgcctcg gtcggcattg
     4441 ccttgcacga agcgcgaatg gcggggcgta tcaagccggg cgatgtggtg atgctggtcg
     4501 cattcggggc cggcctgacg tggggttcga ttctgctgag gatgtaagat gacggcgagc
     4561 gcagccatct tccccggaca aggggcgcaa gaagtaggca tgggcaaaga cgcggcggag
     4621 gtttgcgccg tcgcggccga cactttcgcc caggccgacg agatacttgg gttcgggctt
     4681 tcgcagcttt gcttcgaggg gccggcggag cgtctgaacg cgaccgacat tcagcagccg
     4741 gcgattttca cgaccagtgt cgcgctgtac cgggcggcgc tggaccgcaa gaagattcgc
     4801 ccggaccagt tcgcggccat gggcgggctg agcctgggtg aatacacggc gctgcacctg
     4861 gccggcagcg tgccgttcga tgccgcactg cgcctggtgc agcggcgcgg gcagctcatg
     4921 cagcaggcgg ctcagaggag tcccggcggc atggtctcga tcatggggct gaccgaggag
     4981 caggtgctgg ccgtttgcga gcgggtagcc gagtccggcc gcgtcg
//
LOCUS       DAOSYS010000261         5025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_13901_length_5025_cov_5.260966, whole genome shotgun sequence.
ACCESSION   DAOSYS010000261 DAOSYS010000000
VERSION     DAOSYS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13901_length_5025_cov_5.260966"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="PLU99_13420"
     CDS             complement(<1..1684)
                     /locus_tag="PLU99_13420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO18089.1"
                     /translation="MKTSAKTTHPDITAPAPVERPADSAALPADSAKLPADSAAQPAP
                     GEVAGREAQEKPAPQAEAPFVPPQNYAAFLDELLPRLTAEDLGERQRAQRELERACTH
                     AAAPGNESRRAELCRAMLARLGPETASGARISFVGQLEKVGQADCVPDLGGLLEDRDP
                     QVREAARRALQNMPVPAAGLALREALRRATAPEMQVALINALAARGETVALAVMLDLV
                     NSEQPEVASAALAAVGDLGGVSEVAVLGALLDDADTARRAPAADVIVRIADRLAKAGE
                     GRGAMPALWKISGDPVLPAAVRGAALRVLVVVDPRQALPLVQRALADETTPALRPVAA
                     RLLAEIPGDDVLFRIVMTLNTYPTEVQCILLDVVAERGSKGKLAVLAALDSNEEIVLV
                     AALKALRKVGDVTDIPRLARLAALGEGDVRDAARGTLERLPGFNIEPALMLELDKDQE
                     AGIRAEIIRALAHRNFKLAVPRLLALAEDASPVVRAAAYEALGRLATAEATTQLVGFL
                     TAEKDEDAAKAAEDAIVELAQSDEDRDGRAGPVLAALKDSSGIAAGRLLRVLG"
     gene            complement(1777..3063)
                     /locus_tag="PLU99_13425"
     CDS             complement(1777..3063)
                     /locus_tag="PLU99_13425"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO18090.1"
                     /translation="MTDRNPVTRREFLKGAAKLAAAPYVVSALTLGRGARAAPSERLT
                     LGMTGVGNMGGGHLETLLGHRDVQILAICDVQRDKREAARKKVEEHYAADISSGKYSG
                     CETYNEFEKLLARADIDAVVIAVPDHWHAIVAIAACRAGKDVYCEKPLSLTVREAEQM
                     AATARAYNTVFQTGSQQRSSREFRLACELIRNGRIGKVQTINVGIGMPSTEKCLPEEP
                     VPEGFDWERWLGPAPWAPYNAERCSGDYSGGWRLIRDYSGGMTTDWGAHHFDIAQWAL
                     GMDGDGPVEIIPPGCDGCDTLTFKYASGVVMSRGGANGILFTGTDGKIEVNRGHLKSW
                     PEEIIKEPIGPNEIHLYESPGHHQDWLNCIKTRSRPICDVAIGASSVTVCHLGNIASW
                     LGRPIRWDPQKREIIGDEAASRWLDRPKRAPWRLFL"
     gene            complement(3278..4015)
                     /locus_tag="PLU99_13430"
     CDS             complement(3278..4015)
                     /locus_tag="PLU99_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18091.1"
                     /translation="MSTLTNCCASRTTVLLKSGLLALLATGFCTGGSCTINIDHPGDP
                     GAVAGYVPLDEAFLDLRINASGGTAEATAVITDLLHRIVRLRQGQEVAINGRSLIGPD
                     VAGVYRATLSAAGEYTVTVREPTRGVSNTLLQPPPGFSIVQPPAGGTASLSGFTVAWT
                     NSDPALDVEIRLTQVLFGAERTVTFGPFTDTGTQALSASQLADFRQGAPLLVTVIRIL
                     ESPAVEGFRSAVASVEISETVSVTPTP"
     gene            complement(4047..4919)
                     /locus_tag="PLU99_13435"
     CDS             complement(4047..4919)
                     /locus_tag="PLU99_13435"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOO18092.1"
                     /translation="MKALAKPLVRFWLLMPALLLCSCPAPRLELAPVELENGVEGWDY
                     GEQLVSEGRPPLRWSISQGELPPGLILDDGGALVGTPTQPGDYAFTVRVRDSSFPART
                     GTQNYAITILPRLRITANLPDGRVLEPYAATPAVEGGVPPYTFEAIGLPAGLGIKSET
                     GTISGVPLTPHAALRVDVRVSDSGQPQQTATDTDFFTIKNQAIQIVTSDLPPGQVGVP
                     YSQAVVAENGQPPYRWAVSDGLLPGGLRLNIVTGVISGTPTAPGTSTFTISVIDSDNP
                     ATSDATVFTVEIAP"
BASE COUNT          828 a         1707 c         1622 g          868 t
ORIGIN      
        1 ggcccagcac gcgcagcagg cggccggccg caatcccgct ggagtccttc agcgccgcca
       61 ggaccggccc ggcccgcccg tcgcgatcct catcgctctg ggctaattcg acgatcgcat
      121 cctctgcggc tttggcggcg tcctcgtcct tttctgccgt tagaaagccg acgagttgcg
      181 tggtcgcctc ggccgtagcc agccggccca aggcttcata cgccgcggcg cgaaccaccg
      241 gactggcgtc ttcagccagc gctagcagac gcggcaccgc cagcttgaag ttgcgatgtg
      301 ccagcgctct gatgatctcc gcccggatgc ccgcctcctg gtctttgtcc agttccagca
      361 tgagagccgg ctcgatgttg aacccgggca gccgttccag cgtgccgcgg gccgcatcgc
      421 gaacgtcgcc ctcgcccagg gctgcgaggc gcgccagccg agggatgtcg gtcacgtcgc
      481 ccacctttct gagggccttg agggccgcga ccaggactat ttcctcgttg ctgtcgagcg
      541 ccgcgagcac ggccaacttg cctttgctgc cgcgttcggc cacgacgtcg agcagtatgc
      601 attgcacctc cgtcgggtag gtgttcagcg tcataacgat cctgaagagc acgtcgtcgc
      661 ccggtatttc cgccagcaag cgagcggcaa cggggcgcaa ggcgggcgtg gtttcatcgg
      721 cgagcgcacg ctgcaccagc ggcagggctt gccgcgggtc aactacaacc agaacgcgga
      781 gcgcggcgcc gcgcacggcg gccggcagaa ccggatcgcc cgagattttc cacaacgccg
      841 gcatcgcacc ccgcccctcg ccggcttttg ccaggcgatc ggcgatgcgc acgattacgt
      901 cagccgcggg cgcccggcgc gccgtatcag cgtcatccag caaggctccg agaacggcga
      961 cttcagaaac accacccagg tcgcctacgg ctgcgagcgc ggccgaagcc acttcgggct
     1021 gctcggagtt cacaaggtcg agcatgactg caagcgcgac tgtctcgccg cgcgccgcca
     1081 gggcgttgat aagcgccact tgcatctcag gagcagtggc tctccgcagt gcttcgcgca
     1141 gggccaagcc cgcggcggga accggcatgt tctgcaaagc gcggcgtgcc gcctcgcgca
     1201 cttgcggatc gcgatcttcg agcaagccgc caaggtccgg cacgcagtcg gcttggccga
     1261 ccttctcgag ttgtcccacg aaagaaatcc gcgcgcctga cgcggtctcc ggtcccaacc
     1321 gagcgagcat ggcccggcac agctcagcgc ggcggctctc gtttccgggc gcggcggcgt
     1381 gagtgcaggc gcgctccagt tcccgctgcg ctcgctgacg ctcacccaga tcctcggcgg
     1441 tcaggcgcgg cagaagctcg tccaaaaatg ccgcgtagtt ctgtggcggg acgaacggcg
     1501 cctcggcttg cggcgccggt ttttcctgcg cttcgcgccc ggcaacttcg ccaggcgccg
     1561 gctgcgccgc tgagtcggcc ggcaacttcg ctgagtcggc gggcagcgcc gctgagtcgg
     1621 ccggcctttc aaccggcgcc ggcgcggtaa tgtccggatg cgtggtcttc gcgctcgtct
     1681 tcattgacga ttcagtgcat gaaaacgcca gtagggcgct caaggaagca gcgatagtca
     1741 ctagtctcat agtcatactc ttgctcacac tttcgctcaa aggaacagcc gccacggcgc
     1801 ccgtttcgga cgatcgagcc agcgcgacgc ggcctcgtcg ccaatgatct cacgtttttg
     1861 cgggtcccag cggatcggcc ggccgagcca tgaggcgata ttgcccaaat ggcaaaccgt
     1921 caccgagctg gcgcctatcg ccacgtcgca aatgggccgg ctccgggtct tgatgcagtt
     1981 cagccaatcc tggtgatggc cggggctctc atacaggtgt atctcgttcg gcccgatcgg
     2041 ctccttgatg atctcttcgg gccagctttt gagatggccg cggttgacct cgatcttgcc
     2101 gtcggtcccc gtgaagagaa tgccgttagc acccccgcgg gacatcacca cgccgctggc
     2161 gtacttgaac gtcagcgtgt cgcagccatc gcagccgggc ggaatgatct cgaccggccc
     2221 atcgccgtcc attccgagcg cccactgggc aatgtcgaaa tgatgggccc cccaatcggt
     2281 agtcatgccg cccgaataat cacgaatcag ccgccagccg ccggagtaat cgccgctgca
     2341 gcgttcggcg ttgtaggggg cccacggcgc cgggccgagc cagcgctccc agtcgaaccc
     2401 ctccggcacc ggctcctctg gcaggcactt ctcagtcgaa ggcatgccga tgccaacgtt
     2461 aatcgtttgc actttcccaa tgcgtccgtt gcggatcagc tcgcaggcca gccgaaactc
     2521 ccggctcgag cgctgctggc tgccggtctg gaagacggtg ttgtaggcgc gggcggtcgc
     2581 ggccatctgc tccgcttcgc gtaccgtcag cgagagcggc ttctcgcagt acacgtcctt
     2641 gccggcgcgg caggcggcaa tggccacgat ggcgtgccaa tggtcgggaa cggcgatgac
     2701 gaccgcgtcg atgtccgcgc gggccagcag cttctcgaat tcgttgtacg tctcgcaacc
     2761 gctgtacttt ccggaagaga tgtctgcggc gtagtgctcc tcgactttct tgcgggccgc
     2821 ttcgcgcttg tcacgctgca cgtcgcaaat cgcgagaatc tgaacgtcgc ggtggccgag
     2881 cagcgtctcc aggtgaccgc cgcccatgtt gccgacgccg gtcattccca gtgtcagccg
     2941 ctcgctgggg gcagcccgcg ctcctcgccc caacgtgagc gcggagacga cgtacggcgc
     3001 cgcggccagc ttggcggcgc ccttcaggaa ctcacggcgc gtaacggggt tacggtcggt
     3061 cacatgctcc tccatcgact ccggcccgca ggtctgagcc cgttttgatg gaggaaagct
     3121 tagcgtgatc cggcccgagg cggtagcgtc ggcccccggc ggccgacgta ggtagtagcg
     3181 ccggcccccc gtggccgacg caatgcggat ccgccgtcaa accgctaagg gagcagcgtc
     3241 cctgccaagg ttcaacgtcg cccgcagggc ggcgacgcta cggcgtgggg gtcaccgaga
     3301 cggtctctga gatctcaact gacgctacag cgctccgaaa gccctccacc gcgggcgatt
     3361 cgaggatgcg tatgacggtg accagcagcg gcgcgccctg gcggaagtcg gcgagctgcg
     3421 aggcggacag cgcctgcgtc ccggtatcgg tgaacggccc aaacgtcacc gtccgctccg
     3481 cgccgaatag cacctgcgtc agcctgatct ccacatccag cgcggggtca gaattcgtcc
     3541 aagccaccgt gaagccggaa agcgacgccg ttccgcccgc cggcggctgc acgatcgaga
     3601 accccggcgg cggctgcaga agcgtgttgc ttacgccgcg cgtcggttcg cggacagtca
     3661 cggtgtactc gcccgcggcc gacagcgtag cgcgataaac gcctgcgaca tcggggccga
     3721 tcagcgaccg gccattgatc gcgacttcct gcccttgccg cagccgcacg atgcgatgca
     3781 agaggtcagt tatcacggcg gtggcctcgg cggtgccgcc ggacgcattg atccgcagat
     3841 cgagaaaggc ctcgtccagc ggcacgtatc cggcaacggc cccggggtcg ccgggatggt
     3901 caatgtttat cgtgcatgac ccgcccgtac agaagccggt cgccaacagc gccaataggc
     3961 cggacttcag cagcacagtc gtcctcgacg cacagcagtt tgtcagcgtg ctcacttggc
     4021 gctccttcgg gttttcgcaa tagcgactac ggtgcaatct caaccgtgaa gacggtcgcg
     4081 tcgctggtgg ccgggttgtc cgagtcaatg acactgattg taaacgtgct ggttccgggt
     4141 gcagttggcg ttccggatat cacgcccgtc acgatgttca aacgcagccc gcccggcaac
     4201 aggccgtcgc tgacggccca ccgatagggc ggctggccat tctcggcgac cactgcttgg
     4261 ctgtaaggga cgcctacttg gccgggcggc aagtcgctgg tgacgatctg aatggcctgg
     4321 ttctttatgg tgaaaaagtc agtgtcggta gcggtttgct gcggctggcc gctgtcgctg
     4381 acgcgcacgt cgacacgcaa tgccgcatga ggcgtcagcg gcacaccgga aatcgtgccc
     4441 gtctcggatt tgatgcccag gccggccggc aagccaatcg cctcaaacgt gtagggcggt
     4501 acgccaccct ccacagccgg cgtggccgca tacggctcta acacccgtcc gtccggcagg
     4561 ttcgcggtga tccgcaggcg cggcaggatg gttatggcgt agttctgcgt gccggttcgc
     4621 gcggggaagg acgaatcgcg cacgcgcacc gtgaaggcgt agtcgccggg ctgtgtgggc
     4681 gtgccgacca gcgcgccgcc gtcgtccaga atgagccccg gcggcagttc gccctggctg
     4741 atggaccagc gcaacggcgg gcggccctcg ctaaccagtt gttccccgta gtcccagccc
     4801 tcaacgccgt tctccaattc caccggcgcc agctcgagcc gaggggcagg gcaggaacac
     4861 agtagcagcg ccggcatcaa cagccagaac cgcacaaggg gtttcgcgag cgctttcata
     4921 tccggctctc aatacgtgtc gcgggccgcg acttcgaaac gcctgagctc atgccgcatt
     4981 atactggcag tggtcaaccg ccgcgggtgg gtgcatgctt gggtg
//
LOCUS       DAOSYS010000262         4917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14274_length_4917_cov_6.079391, whole genome shotgun sequence.
ACCESSION   DAOSYS010000262 DAOSYS010000000
VERSION     DAOSYS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14274_length_4917_cov_6.079391"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..2494)
                     /locus_tag="PLU99_13440"
     CDS             complement(134..2494)
                     /locus_tag="PLU99_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOO18093.1"
                     /translation="MNRNRPRRARPAVRKGKTRASRRGIASILIVLAACGAMAAWFFA
                     HGAARGGLGRLHVDQNLRCESRFPAGPGALAGSNVLLITLDTTRADYLGCYGGSVQTP
                     ALDDLARRGVLFSQALTPAPITLPAHASLLTGLYPHHHAARTNGFYRLEDTHKTLARE
                     LKNAGYATGAIISAMVLGSRYGLAAGFDNYHDDFSDARPQPQGYEAERRADRTTDHAV
                     AWLREHCGGPFFLWVHYFDPHVPYDPPPPYAQQYAQNPYAGEIAFMDAQLGRLLDALQ
                     QLGLGDNTLVIAAGDHGESLNEHGEGAHGYLLYNPTVHVPLIMACGQRLGGGVHVPGL
                     VSLTDVMPTALSLLGLPPPDADGVDLTRVEPTGPVYCESLYSLVEQGWAALFAVYVDQ
                     QKYIFSPNPQLFDLSADPHELRNLVASQPETAARMLQRVSDFFGEELNRPGAPAPMQE
                     LDDVEIAKLRSLGYVGTRAPDDFADRPRPDPAVLLPLLQRMQFAVSGALGGHIPFQDA
                     VKTLQDIIQNRPDFHPAYQYLADLLNDMGDLEQAAAIARRGLEIAPNNTALLLSLAQV
                     ESQRGNAVEAADLFRRVLAAYPESFDAQIGLGLALLGGGDSGDALEVFTDLAEVAPSD
                     PHVCDGLARAAIASQRMDEAVAVLTAAIKDQPAVVSPRLALAAIKRTQKLCWQAAKVL
                     REGLALTPGQPQLTDALATTLLQTGTQSYDPGEAARLMERLCQRTQYKEPQFMLTLSA
                     AYYHLGRAEEAISVAQTAQQLAANAGYDALAQRIGRAVERYAQSGG"
     gene            complement(2859..4538)
                     /locus_tag="PLU99_13445"
     CDS             complement(2859..4538)
                     /locus_tag="PLU99_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOO18094.1"
                     /translation="MPLGEALVAKGLITREQLQQAVAERSSASERLDQHLVRTGLVRE
                     RDILRVLSEELDIPIVEPDEADVDRELLKAVPSRLVHKYGIIPISRNGRGIRVATSDP
                     YNVYALDELRTCINTPIEPVLASSTVIHKLIKSFYGVGGEVLTTLVQERGELEVVDEH
                     KIESADLDVQMAQEASVVKLVNEILLEAIDQRCSDIHFEPFENDFVVRYRVDGVLHVA
                     NVPPEIRRFQNAIISRIKILSNLNIAERRLPQDGGFKIKAHGREIDLRVSVIPMAFGE
                     GIVLRILDRSAVRLDLETLGMDGQVLESFTECIQRPHGIILVTGPTGSGKTTTLYAAL
                     KRIVSPELKILTIEDPVEYYLEGINQTQTLPKIGLTFARALRSFLRHDPDVILVGEIR
                     DLETAEVAINASLTGHLVFSTLHTNDAVTATTRLLDMGVEPFLVSSSIEAVLAQRLVR
                     TICKECKQEWRPSHARELPPDFELGPKDKLYKGAGCRECHNTGYAGRRALFELLVMSD
                     NLREMVIKRQSASQILKAAREEGLVLMREAGWDLCRRGLTTPQEVLRVTKA"
BASE COUNT          877 a         1510 c         1616 g          914 t
ORIGIN      
        1 gtgatggccg cggccagccc gttggggccg gcgccgacga cgatgtaatc cgctgccgcg
       61 ttctggtcca tgaaggattg tcgcgcgcag acgtgaaaat tctgcggagg cgccgcggct
      121 cggcgccggc gatttagccg ccggattgag cgtagcgttc aacggcccgc ccaatgcgtt
      181 gggcgagcgc atcgtacccg gcattggccg cgagctgctg tgcggtttgc gctaccgaaa
      241 tggcctcctc ggcgcggccc aagtggtaat acgcggcgct caaggtcagc ataaattgcg
      301 gctctttgta ctgtgtgcgt tggcagaggc gttccatgag gcgggccgct tcgccggggt
      361 cgtaggactg ggtgccggtc tgcaatagcg tcgtggcgag ggcgtccgtc aactgcggct
      421 gcccgggcgt cagcgccaat ccctcgcgta gcaccttggc agcttgccag cacagcttct
      481 gcgtccgctt aatcgcggcc aaggccaggc gcggcgagac gaccgccggc tggtccttta
      541 tcgccgcggt cagcaccgcg accgcctcgt ccatgcgctg cgaggcaatc gcggctcgcg
      601 ccaggccgtc gcacacgtga gggtcggacg gggccacttc cgccaaatcc gtgaatactt
      661 ccagagcatc gccggagtcg ccgccaccca acagcgccag gcccaggccg atctgggcgt
      721 caaatgactc ggggtaagcg gccagcacgc ggcggaagag gtccgcggcc tcgaccgcgt
      781 ttccgcgttg cgactcgacc tgcgcgagcg acaatagcag ggcggtattg ttgggggcga
      841 tttccaagcc gcggcgggcg atggcggcgg cttgctccag gtcgcccatg tcattcagca
      901 ggtcagccag gtattggtag gctgggtgga aatcgggccg gttttggatg atgtcctgaa
      961 gagttttgac cgcgtcctgg aaaggaatgt gtccgcccag cgcgccggac acggcgaact
     1021 gcattctctg caatagcggc aacaggacgg ctggatcggg tcgtggccgg tctgcgaaat
     1081 catcgggggc ccgcgtacca acgtacccga gggaccgcag ttttgcgatc tcgacgtcgt
     1141 ccagttcctg catgggcgcc ggcgcgccgg ggcgattcaa ctcttcgccg aaaaagtcgc
     1201 tgacgcgctg cagcatgcgg gccgcggtct caggctgact ggcaacgagg tttcgcaatt
     1261 catgaggatc agccgacaag tcgaagagct gcggattagg gctgaagatg tacttctgct
     1321 gatcgacgta cacggcaaag agggccgccc agccctgctc gaccagcgag tacaacgatt
     1381 cacagtaaac ggggccggtc ggctcgaccc tggtcaggtc cacaccgtcg gcgtccggcg
     1441 gcggcaggcc cagcagggaa agcgcggtcg gcatcacatc cgtaagtgag acgaggccgg
     1501 gaacgtggac gccgccgccc aggcgctgcc cgcacgccat gatcagaggc acgtgtaccg
     1561 tcgggttgta aagcaggtag ccgtgagccc cctcgccatg ctcgttcagg ctttcgccat
     1621 gatcgccggc ggcgatgacc agtgtgttgt cgcccaggcc aagctgctgt agggcgtcga
     1681 gcaggcggcc gagctgtgcg tccatgaacg cgatttcgcc cgcgtagggg ttttgggcgt
     1741 attgctgcgc ataaggcggc ggcggatcgt acggcacatg cggatcgaag taatgcaccc
     1801 agaggaaaaa gggaccgccg caatgttcgc gtagccaggc cacggcatgg tcagtagtgc
     1861 ggtctgctct tcgctcagcc tcgtagccct ggggctgggg cctggcgtcg ctgaaatcgt
     1921 cgtgataatt gtcaaagccc gcggcgaggc cgtagcgcga gcccaagacc atcgccgaga
     1981 ttatcgcgcc cgtggcgtag ccggcgttct tcagctcacg cgccagcgtt ttgtgcgtgt
     2041 cttccagccg gtaaaacccg ttggtccgcg ccgcgtggtg gtgcgggtag aggccggtca
     2101 gcagcgaggc gtgggccggc agcgtgatcg gggcgggcgt aagggcctgc gagaatagca
     2161 ccccgcgccg ggccagatcg tccagggccg gtgtttgcac cgaaccgcca tagcagccga
     2221 ggtaatccgc gcgagtggtg tccaacgtga tcaacaggac gttggagcca gccagagcgc
     2281 ccggtccggc cgggaaacgc gactcacaac gcaggttttg gtcaacatgc agccggccca
     2341 agccgcctcg cgctgcgccg tgcgcgaaga accacgctgc catagcgccg caagcggcca
     2401 aaacgatgag gatactcgca attccccggc gcgatgcgcg cgttttccct ttgcgcacgg
     2461 ctggccgcgc gcgtcgcggt ctgttgcggt tcatgccttc actccttgcc cgtgggtgcc
     2521 tgggtgccta ggccaggccg gttttcaccg gtggagcacg ggcccgctcc cgcttgcagt
     2581 ggtcggggca gtgggagcgg ttgcgctcat cttaccgaat cggcacgcga taaccaagac
     2641 ttttctcgcc cacacctgcc ctgtgctgcg ggccctgtta tcgggtactg ccgaagtgct
     2701 cccccaaaag gcgagttgca gatcatcacc aggcaatatc gaggatcaac aatcccgatc
     2761 gacgcaccgc gacactctgc ctgcagcgag agtcgcgcgc ggccatttcc agtcggccga
     2821 ttcgactccc agtgtttagt gtttgacgct gaaccggatc aggccttggt gacgcgcagc
     2881 acttcctgag gcgtcgtcaa cccgcggcgg cagaggtccc agccggcctc gcgcatcagc
     2941 accagaccct cttcgcgggc cgctttcaga atttgcgacg ccgactggcg cttaatgacc
     3001 atctcgcgca ggttgtcgct catgaccaga agctcgaaca acgcccgccg tccagcatag
     3061 ccggtgttgt ggcactcgcg gcagccggcc cctttgtaca gcttatcctt gggccccagc
     3121 tcaaaatcgg gcggcagctc gcgagcgtgc gacggcctcc actcctgctt gcactctttg
     3181 cagatggtgc gcaccaggcg ctgcgctaga accgcctcaa tcgagctgga aacaaggaac
     3241 ggctcgacgc ccatgtccag caaacgcgtg gtcgccgtaa ccgcatcgtt cgtgtgcagc
     3301 gtgctgaaca ccaggtggcc cgtgagcgag gcgttaattg cgacttcggc cgtctctaag
     3361 tcacgaattt cgccgaccag gatgacgtcc gggtcgtgac gcaggaaact gcgcagcgcg
     3421 cgggcgaacg tcaggccgat cttgggcagc gtctgcgtct ggttaatgcc ctccaggtag
     3481 tactcaaccg ggtcttcgat cgtcaaaatc ttcaactcgg ggctgacgat gcgcttgagc
     3541 gccgcgtaca gcgtcgtcgt cttacccgag ccggtcggcc cggtaaccag aataatgccg
     3601 tgtggccgct ggatgcactc cgtgaacgat tccagaactt gcccgtccat gcccagcgtt
     3661 tccaggtcga ggcggacggc ggagcggtcg aggatacgca gcacgatgcc ttcgccgaag
     3721 gccatcggaa tcacgctcac gcgcaaatcg atctcgcgcc cgtgggcctt gatcttgaag
     3781 ccgccatcct gcggcaggcg gcgctcggcg atgttcaggt tcgagaggat tttgatgcgg
     3841 ctgatgatcg cgttttggaa gcggcggatt tccggcggca cattggccac gtgcagcacg
     3901 ccgtcgacgc gatagcggac cacgaaatca ttctcgaacg gctcaaagtg gatatcgctg
     3961 cagcgctgat cgatggcttc gaggagaatc tcgttgacca gtttgacgac cgaggcctcc
     4021 tgggccatct gcacgtcgag gtcggccgat tcgatcttgt gctcgtcaac gacctccagc
     4081 tcgccgcgct cctggaccag cgtcgtgagc acttcgccgc cgacgccgta gaaagattta
     4141 atgagcttgt gaatgacggt gctggaggcg aggacgggct cgatcggcgt gttgatgcag
     4201 gtccggagct catccaacgc gtaaacgttg tatggatcag aggttgcgac gcggatgccc
     4261 cttccgttgc gcgatatcgg aatgatgccg tacttatgga ccagacggct gggcacggcc
     4321 ttcaggagct cgcggtcgac gtcggcctcg tcgggctcga cgatgggaat gtcgagttct
     4381 tcgctgagga ccctcaggat gtcgcgttcc cggactagtc cggtccggac caagtgctga
     4441 tccaggcgtt ctgaagcgga cgaacgctcc gcgacggctt gttgaagctg ttcgcgggta
     4501 atcaggcctt tcgcgaccag ggcttcgcct aggggcattt gtgtaacctc ggtgctatca
     4561 agaggttgcg tgcagccagc gcatgcgcag gcgcaattca cgcgcggtgt tataccacga
     4621 aagcccggcc tgattgcagt cgtacggtaa attcatctgc gatctacaaa cggcacccgc
     4681 aaagtgctat tcgccgcatt aaccaaaaac ggcgagaaaa tggcggcggc ggaacgtgca
     4741 ggtccggtat cttgctggtc taataccagg ggcgtgcgac gagccataac gtccggtatg
     4801 cggaccgctg agaggatgac gatgaaccga cgtgggttac gcctgctatt tctgaccttc
     4861 gtcgtgcccg gattggctgt ggccgtcggc tgtgatcgaa cggctcccgc gggcgga
//
LOCUS       DAOSYS010000263         4884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14397_length_4884_cov_5.189687, whole genome shotgun sequence.
ACCESSION   DAOSYS010000263 DAOSYS010000000
VERSION     DAOSYS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14397_length_4884_cov_5.189687"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLU99_13450"
     CDS             complement(<1..281)
                     /locus_tag="PLU99_13450"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOO18095.1"
                     /translation="MRVLSQAELESALGEVVSRLRGALSPVAIYLFGSYAYGTPRRGS
                     DIDLLVVVEDSPLDAYARDAIAYRALGRIPLPIDVQVYTRAEFEERSALP"
     gene            complement(322..2181)
                     /locus_tag="PLU99_13455"
     CDS             complement(322..2181)
                     /locus_tag="PLU99_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOO18096.1"
                     /translation="MNTRDDRENSAKAEAPRRDFIREIIDEHNRTGRFGQRVHTRFPP
                     EPNGYLHIGHAKSICLNYGLAEEYGGKFNLRMDDTNPEKEETEYVNSIIEDVRWLGAR
                     WVGMREDDPRAGVLWASNYFDQMYEFAIEAIKKGRAYVCDLTAEEVSRTRGTLTTPGQ
                     ESPYRNRSVEENLELFERMRRGEVPDGTKTLRAKIDMAHPNLNMRDPVMYRIVHATHH
                     NTGDRWCIYPMYDWAHGFEDSLEGITHSICTLEFENHRPLYDWFIDMINEGRTDDGSG
                     PWGKKIHHPQQIEFARLNLTYTVMSKRRLLQLVKEGYVRGWDDPRMPTVSGMRRRGFT
                     PEAIREFCRRIGVSKVESTVDVALLEHCVREHLNKVCPRVMGVLRPLKLIIDNYPPGQ
                     VEWMEAVNNPEDPAAGTRKVPFARELYIEQDDFREVPPPKYWRLYPGNEVRLRYAYFV
                     KCTGCDKDPQTGEITAVHATYDPATRGGDAPDGRKVKTTLHWVSAQHALPVEVRLYDH
                     LFTKPDPDDVSDDAIRAAESGGSAEQQAVEAAAAAKDWKSNLNPKSLETLEAAYVEPS
                     VVDAAPGARYQFERLGYFCVDPDSGNPGASSRRLVFNRTVTLKDEWAKIAKKQ"
     gene            2370..2948
                     /locus_tag="PLU99_13460"
     CDS             2370..2948
                     /locus_tag="PLU99_13460"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOO18097.1"
                     /translation="MTTIIRPNLEHAGEYRGLRMTADEFLALPESKCHYELINGIVTM
                     SPSPSMRHQQIVREILVQLATFLRGRGLGHAVHDVDVRFAADLVYRPDVIYLSAEKFA
                     RCSTRVTEIPDLVVEVISPDSRRYDHETKKHDYERYGVQEYWLVDADERSFTLYRREG
                     ERFVPVLAAGERLPSSIIAGFELELTPIRSLF"
     gene            complement(3947..>4274)
                     /locus_tag="PLU99_13465"
     CDS             complement(3947..>4274)
                     /locus_tag="PLU99_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18098.1"
                     /translation="GLKLLWTGKHAAPNLNDDMQCFLTIVAGAASPELTRLVENNAAA
                     LNRIRSAAGRPYVVWPPAHGAGGLDPERRSYELLILIRLCYVAALHAHIAGDDGAAFA
                     DVHRGL"
     assembly_gap    4275..4284
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4666..>4884)
                     /locus_tag="PLU99_13470"
     CDS             complement(4666..>4884)
                     /locus_tag="PLU99_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18099.1"
                     /translation="PPTVVYGADVLLGTLLFGGTPAWVDLGYTPGDLTEGFATPGGFA
                     QATFSGWYIPEPAALALLACSALLLRRR"
BASE COUNT          884 a         1518 c         1536 g          936 t
ORIGIN      
        1 ggaagagccg agcgttcttc gaactccgct cgcgtatata cctgcacgtc tatgggtaag
       61 gggatcctgc cgagggcacg gtaggcgatc gcatcgcgcg cgtaagcatc gagcggacta
      121 tcttccacga ccaccaggag gtcgatgtcg ctgccgcgac gcggcgtgcc gtaggcatac
      181 gagccgaaca ggtagatcgc gaccggcgag agcgcaccgc gcagacggct gacgacctcg
      241 cccaaggcgc tttcaagttc tgcctgactc agcactcgca tggtcaagac ccagaacagt
      301 cacctgcaag ccaccagaat tctactgttt cttcgcgatt ttggcccact catctttcag
      361 cgttaccgtg cggttaaaga ccaggcgtcg gctactggcg cccgggtttc cgctatccgg
      421 gtccacgcag aagtagccca ggcgctcgaa ctggtagcgc gccccgggag cggcgtccac
      481 cacgctgggc tcgacgtacg ccgcctcgag ggtttcgagc gacttcggat ttaggttact
      541 cttccagtcc ttggccgccg cagccgcttc gaccgcctgc tgttcagcgc tgccgccact
      601 ttcagctgcg cgaatggcgt cgtcgctgac gtcgtcggga tccggcttgg tgaagaggtg
      661 gtcgtagagg cgcacctcga ccggcagcgc gtgctgggcg ctgacccaat gcagcgtcgt
      721 cttcaccttg cggccgtcgg gggcgtcgcc gccgcgggtg gccgggtcat aggtcgcgtg
      781 gacggcggtg atttcgccgg tctgcgggtc cttgtcgcag cccgtgcact ttacgaagta
      841 ggcgtagcgc aggcgcacct cgttgcccgg atacagccgc cagtacttcg gcggcggcac
      901 ctcgcgaaag tcgtcctgct cgatgtacag ctcgcgcgcg aacggaactt tgcgcgtccc
      961 ggcggccggg tcctccgggt tgttcaccgc ctccatccac tcaacttgcc cgggcggata
     1021 attgtcgatg atgagcttca gcggtcgcag cacgcccatg acgcgcgggc agaccttgtt
     1081 gaggtgctcg cgcacgcagt gctccagcag cgcgacgtcg accgtgctct ccacctttga
     1141 aacgccgatc cgccggcaga attcgcggat ggcttccggc gtgaagccgc gccgccgcat
     1201 gccggaaacg gtcggcatgc gcggatcgtc ccagccgcgt acatagccct ctttgacgag
     1261 ttgcagcagc cgccgcttgc tcatcacggt gtacgtcagg ttgaggcggg cgaactcgat
     1321 ctgctgcggg tggtgaattt tcttgcccca cggcccgctg ccgtcatccg tgcggccctc
     1381 gttgatcatg tcgatgaacc agtcgtacag cgggcggtga ttctcaaact cgagcgtgca
     1441 gatactgtgg gtgatacctt cgagcgagtc ctcgaacccg tgtgcccagt cgtacatcgg
     1501 ataaatgcac cagcggtcgc cggtattgtg gtgcgtggcg tgcacgatgc gatacatgac
     1561 cgggtcgcgc atgttcaggt ttgggtgggc catgtcgatc ttggcgcgca gggtttttgt
     1621 cccgtcgggc acctcgccgc gccgcatacg ttcgaacagc tccaggtttt cctcgacgct
     1681 gcgattgcga tacgggcttt cctgaccagg cgtggtcaac gtgccgcgcg tgcggctgac
     1741 ttcttcggcc gtcaggtcgc agacgtaggc cctgcccttc ttgatcgcct cgatggcgaa
     1801 ctcgtacatc tggtcgaagt agttcgaggc ccacagtacg ccggcccgcg ggtcgtcctc
     1861 gcgcatgccg acccagcgcg cccccagcca gcgcacgtcc tcgatgatcg agttgacgta
     1921 ttcggtctct tccttttccg ggttggtgtc gtccatgcgc aggttgaact tgccgccgta
     1981 ctcctccgcc aggccgtagt tcaggcagat gctcttggcg tgcccgatgt gcaggtagcc
     2041 gttcggctcc ggaggaaagc gcgtgtgcac gcgttgcccg aagcggccgg tacggttgtg
     2101 ctcgtcaatg atctcgcgaa tgaagtcccg ccgcggcgct tcggccttgg cggagttctc
     2161 acgatcatcg cgtgtattca tgctggcctt tctcggtcga ggaacgcgcc tcagggtcgt
     2221 caatgctgaa aaatacgaac gtgggatcat aatcagcctg aagcacgggg acaaaccggg
     2281 cctgcgccgc cggcacaagc gcgtttagaa tctctgcgcc gcaggcaagg ccggctgtat
     2341 aatggctgcg cttgcaggga gacgcctgag tgacgacgat aattcgaccc aatcttgagc
     2401 acgccggcga ataccgcggc ttgcgaatga ccgcggacga attcctggct ttgcccgaga
     2461 gcaagtgcca ctacgaactg attaacggaa tcgtcaccat gtcgcccagc ccgagcatgc
     2521 gtcaccagca gatcgtgcgg gagattctcg ttcaactggc cacctttctg cgcgggcgcg
     2581 ggctcgggca cgccgtccac gacgtcgacg tgcgctttgc ggcggatctc gtctaccggc
     2641 ccgacgtcat ctatctgtcg gccgagaagt tcgcgcgttg cagcacccgg gtgacggaaa
     2701 tccccgacct ggtcgtcgag gtgatttcgc ccgattcccg ccgctacgac cacgaaacga
     2761 agaagcacga ctatgagcgc tacggcgtgc aggagtactg gctggtggac gccgacgagc
     2821 gaagcttcac gctctaccgc cgcgagggcg agcgcttcgt gccggtcctc gcagcgggcg
     2881 agcgcctgcc cagttccatc atcgccggct tcgaactgga gctgacgccg atccggtcgt
     2941 tattctgagc cagtttcata ccatgcgtcc ccgtttcaca cggctgcgcg ccgcggcgtg
     3001 gggtcaggtg atcagatgct catgctttgt gcactcgcgc ccgcggcgcg gtttctgcta
     3061 ggtatcatag acagcccgca gctatcagct atggccttca gcccggagtg cgtggattgt
     3121 gcgtgcttgc gcgtcaatgg cggggtggga gcaagagccg gaaggcgata tcgtccccag
     3181 gttcgggttt ggcgccatcc agtgccgatt tagtatcctg tcggccattt ggcccaacgc
     3241 tgtagaggat cgccgcggac acgccagcaa tgtagcccgg ctcggcacac tgcgcaaatg
     3301 ggtccttggg gacgttgacg atgtaatgcg gcacgaggtc cgataggctg ccggggcgat
     3361 ccatggtcgt attcgaacaa gtaaatggct accgcaatcg cggccagacg ccggcgctcc
     3421 aggtgagcaa ccgtgggttg gaggagttgt gaatagttgg gtagcagatg caacgacggc
     3481 tgatgtgtta agcgataaag tcgcgagctc cacggctcct ggcccccgcg tggcgtcatt
     3541 agcgcgggag taaatgagga gctcgcccag gggatttgag caagcgcgct cacatgttca
     3601 tagatccgca ggacatccag ttgccaggcc ggcttcaggc aaagaaggag tattcgatct
     3661 ctgcacgtcc gcccggttgg agacatgagc tcgaggcact ccttatacaa tcggccacgc
     3721 tcagacaaca gcgcaagttt ccagtcgctt tcgaactctg cgtcatcgag tagcaggcgc
     3781 ttcagtttgt caagttcgga gcgcgcctga gtttcctttt cccgatcgac agaagcggca
     3841 cgggtgataa gcatgtgctc cagcacgcac agcccttctt gctcggaacc cacgcgaaat
     3901 aggaagctaa gcaccgaagg ttgcagcgcg tgcatatgtc gggcgttcac aaaccgcggt
     3961 gaacgtccgc gaacgcagcg ccatcgtcgc ccgcaatgtg cgcgtgcagg gccgctacgt
     4021 aacataatct tatcagaatc agcagctcat agctgcggcg ctccgggtct aggccgccgg
     4081 caccgtgcgc cggaggccaa accacatatg gccggccggc tgccgatcgg atccgattca
     4141 gggcggcggc gttgttttca accaacctgg tcagttccgg cgaggccgcg ccggcgacga
     4201 tcgtcaggaa gcattgcata tcgtcgttaa ggttcggcgc agcgtgtttg ccagtccaca
     4261 gtagcttcag ccctnnnnnn nnnngccctt gcaggagctt tccggcagcc gaagaccaga
     4321 gcgccacatc cggatacgtg aagtcgtcct gtaagacggg ttcgcctgcg gcccgggagc
     4381 gctccaatgc cgccgtcaaa cggtgttccg ccagtaagcc ccaacagagg cgggcggtca
     4441 ccaacagagt gcaaactgcg gcacccaaga ttgcgaggcg cttcaaccag cggaaccgtc
     4501 ttggaacgcg cacgtcattc acaacaggca gagttcgaaa tggggacgca gttcgcgggc
     4561 ggttcagaac ggggacgcaa ttcggagttc gaaacgggga cacaatccga aacgggcacg
     4621 tcacgcggcc ccgtttcaca ctcacagcgt ttctagaaca cgggcctagc ggcggcgcaa
     4681 taacagcgcg ctgcacgcca gcaaggccag tgcggccggc tccggtatgt accagccgga
     4741 gaaggtcgcc tgtgcgaatc cgccgggggt cgcgaagcct tcggtcaggt cgccgggcgt
     4801 atagcccaga tcaacccagg ccggggtgcc gccaaaaagc agcgtgccga gcaacacgtc
     4861 tgccccatac acaaccgttg gcgg
//
LOCUS       DAOSYS010000264         4836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14560_length_4836_cov_5.143275, whole genome shotgun sequence.
ACCESSION   DAOSYS010000264 DAOSYS010000000
VERSION     DAOSYS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14560_length_4836_cov_5.143275"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PLU99_13475"
     CDS             <1..796
                     /locus_tag="PLU99_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOO18100.1"
                     /translation="DWQPGANRMHVQSPSLYVMKPFGGSWVAEGSLVYDAMSGASPRY
                     YNTLSGASVTDYRTAGDAKVTKYFDRYAIGVSGVVSSERDFLSRCTGCGACMQICPTG
                     GLQPTLTEAGLAGLWTPRLAPRIGYCDYECNRCGQVCPTEAIMPLPLKEKQQVRIGLA
                     SFDTTRCIPYALGRECAVCEEHCPVPRKAIYLVETEVLTPRGQRRVVKQPRINAGHCI
                     GCGTCEHVCPFKDRPAIRVTSANESRHSDNQPILPGSHGGGSSPHP"
     gene            912..2453
                     /locus_tag="PLU99_13480"
     CDS             912..2453
                     /locus_tag="PLU99_13480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO18101.1"
                     /translation="MKIALISTYAHPIALGMRYVSSSLKAAGHDVKILLMSSRRDTAK
                     PDWSRAAVEALIEHLRDRDLIGLSLVTNTFHRACVLTQRIREAGLRAPIIWGGTHPTV
                     APDESLAIADAICVGEGEHALRELAARLEGGGDPLTVPSLSFRAGSAFGNSEVVRNRL
                     APLEQDLDRFPFPDWDLSTQWVAAKDGLVPARPENLRGALDTLRVETSRGCPFHCTFC
                     NNTGLQILHQGCGKWTRLRSIENVLAELREARARFPGIRAINFVDDLFLVRREEELEE
                     FTARYNEQIGLPLELDAFPNTVTERKVATLARLPIQLVSMGIESASQDTLENIYKRPT
                     PLKRVAEAIALFKKYRIRTEYHYLVSNPFEPEQNVIETMRFIADHHRGRAVLRVFPLM
                     FYPGTPLHKRACESGLIALRDVAAYDYMGTGALQFARHDYLAIWLRVVLSLRNIGAPS
                     WLAHGIINFATSGLVRRLLDHRWFCPSVFAGYFIGRKLWRNFIYQPFIRPFKYLRGRP
                     QAATT"
     gene            complement(2464..3573)
                     /locus_tag="PLU99_13485"
     CDS             complement(2464..3573)
                     /locus_tag="PLU99_13485"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOO18102.1"
                     /translation="MSIKPDKLSRIGVLTGGGDCPGLNAVIRVVTKAAILQLGLQVMG
                     IEDGFLGLIRNRMRPLEMDDVSNILTVGGTILGTSNKANPARFVTGHDADGNPIFEDV
                     TRRVLEHIQERHLDGLICIGGDGTMSVAAHMIRHGVPCIGVPKTIDNDLMHSDITFGF
                     LTAVETATQALDRIHSTASSHHRVMAVELMGRNAGWLALHAGLAAGADVILIPEIPYD
                     IAAVCEKCLQRSRHGKRFTLIAIAEGARPKGGQRVVSQVVRDSPDPIRLGGISNVLVE
                     EITARTGLEARATILGHVQRGGTPIAADRVLAMTFGHKAIQLVASGQWNNCVVRQNGE
                     ATFVPIEAVAGQQRTVPLDDPLLDMARAVGTALGD"
     gene            complement(3673..4689)
                     /locus_tag="PLU99_13490"
     CDS             complement(3673..4689)
                     /locus_tag="PLU99_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOO18103.1"
                     /translation="MRPGSETGRSAGLAVIAFLLVAAAITYLGPVLTPVLIALFLYYI
                     IRPAADALVRRGAPAWLAYLFLFTLTVVAIIWLGRIVHGNMLLVRGRLPEYRANLLGL
                     LDALPGPQARAQAKDLVAQVFDVSTGDVLGLAFGPAISFLETAVMVFFYLFFIIISAR
                     RIGGRVQRAFAPETAGRLLQIGSNINKGIAQYMKVKTLVSLGIAICQALIAYLFGLHF
                     WPLWAVLMFALNYITYVGSIAALVPPVVLAFLQFGNSWTALGLAALLIINRFVWVDYI
                     EIRLAGKSLNVDPVLLLASLAYWGWVWGAVGLVLAAPMLMALKITLANIESTRRWAVL
                     ISDE"
BASE COUNT          893 a         1546 c         1547 g          850 t
ORIGIN      
        1 cgactggcag ccgggcgcga accggatgca cgtgcagtcg ccgtcgctct acgtgatgaa
       61 gccgttcggc ggaagctggg tcgccgaggg cagcctcgtc tacgacgcga tgtcgggcgc
      121 ctcgccgcgc tactacaaca cgctgtcggg cgcttcggtc accgactacc gcaccgccgg
      181 cgacgccaag gtcacgaagt acttcgaccg ctacgcgatc ggcgtctccg gcgtcgtgtc
      241 gtccgagcgc gacttcctgt cgcgctgtac cggttgcggg gcgtgcatgc agatctgccc
      301 gaccggcggt ttgcagccca cgctgacgga ggcgggtctg gcgggtttat ggacgccacg
      361 gctggctccg cgaatcgggt actgcgatta cgagtgcaac cgttgcggcc aagtgtgccc
      421 gaccgaagcc atcatgccgc tgccgttgaa ggagaagcag caggtgcgca tcgggctggc
      481 gtcgttcgac accactcgct gcataccgta cgccttggga cgcgaatgcg ccgtatgtga
      541 agagcactgc ccggtgccgc gcaaggcaat ctacctggtg gaaacggaag tactgacgcc
      601 gcgcgggcag cgacgagtcg ttaagcagcc gcgcatcaac gctgggcatt gcatcggctg
      661 cggtacttgt gaacacgtct gtccattcaa ggatcgcccc gcgattcgcg tgacaagcgc
      721 aaacgaatcg cggcattccg acaaccagcc aatcctcccg ggcagccacg gcggcggcag
      781 ttcgcctcat ccgtagcgcc ggcacgtcgt aagagcatcg cgccgactac ataacttgga
      841 cttagtgcct cggaaatacg tcttgacctt gacgcgggtc gcactaaggt gtgcgcgaca
      901 cgggaggcgg tatgaagatc gcgctcatct ctacatacgc ccatccaatc gcattgggca
      961 tgcgctatgt ctcctcgtcc ctaaaagcgg ccgggcatga cgtcaagata ctgctcatgt
     1021 cgtcgcgccg ggacacggcc aaaccggact ggagccgggc ggcggtcgag gccctcatcg
     1081 agcatctgcg cgaccgcgac ttgatcggcc ttagcctggt gacgaacacg ttccaccggg
     1141 cgtgcgtcct gacgcaacgg attcgcgagg ccggactgcg cgcgcccatc atctggggcg
     1201 ggactcatcc gaccgtagcg cctgatgaat ctttggccat tgcagacgcg atttgcgtcg
     1261 gtgaggggga gcacgcgctg cgagaactgg cggcgcgcct ggaaggcggc ggtgatccgc
     1321 tcacggtgcc gagcctgtcc ttccgcgccg gctcggcctt cggcaacagc gaagtcgtgc
     1381 gcaaccggct ggcgccgctc gagcaggacc tggaccgctt tcccttcccg gattgggact
     1441 tgtccacgca gtgggtcgct gccaaggacg ggctcgtgcc ggcgcgcccg gaaaacctgc
     1501 gcggcgccct ggataccctg cgcgtcgaga ccagccgcgg ctgcccgttt cactgcacgt
     1561 tctgcaacaa caccggcctg cagattctgc atcaaggctg cggaaagtgg acgcggctgc
     1621 gctcgatcga gaatgtgttg gccgaactgc gagaagcccg ggcgcgtttt ccgggcattc
     1681 gggcgatcaa cttcgtggac gacctgtttc tggtgcggcg cgaggaagaa ctggaggagt
     1741 tcaccgcccg atacaacgaa cagattgggc tgccgctgga gctggatgct ttccccaaca
     1801 ctgtcaccga gcgcaaggtc gcgacgctgg cccgactgcc aatccagttg gtcagcatgg
     1861 gcatcgaaag cgccagccag gatacgctgg agaacattta caagcgcccg acgcctctga
     1921 agcgggtggc tgaagccatt gccctcttca agaagtaccg cattcgaacg gaataccatt
     1981 acctcgtcag caatcccttt gagccggagc agaatgtgat cgagacgatg cgcttcatcg
     2041 ccgatcatca ccgcggccgg gcggtgctgc gcgtcttccc gctcatgttc tatccgggga
     2101 cgccgctgca taagcgggct tgcgagagcg ggttaatcgc cttgcgcgac gtggccgctt
     2161 atgactacat gggtaccggc gcactgcaat ttgcgcggca cgactacctg gcgatctggc
     2221 tgcgcgtggt gctaagcctg cgtaatatcg gcgccccctc ctggctggca cacggcatca
     2281 tcaacttcgc gaccagcggg ctggtgcgcc gcctattgga ccatcgttgg ttctgtccga
     2341 gtgtgttcgc aggttacttc attgggcgca agttgtggcg caacttcatc tatcagccct
     2401 tcattcgtcc attcaagtac ctgcgtggca gaccgcaggc cgcaacgacc tagtgcgaat
     2461 tgttcaatca ccgagtgcgg tgccgacggc gcgagccatg tccagcagcg gatcgtccaa
     2521 gggcacagtg cgctgctgcc cggccacggc ttcgatcgga acaaacgtgg cctcgccgtt
     2581 ctgcctgacc acgcagttgt tccattgccc gcttgcgacc agttgaatgg ccttgtgccc
     2641 gaacgtcatg gccagcacac gatccgcggc aataggcgta ccgccgcgct gcacgtgccc
     2701 caggatagtg gcccgcgcct cgagccccgt gcgggccgtg atttcctcaa ccagcacgtt
     2761 gctgatgccg cccaggcgaa tggggtcggg gctgtcgcgc actacctggc tcacgactcg
     2821 ctgcccgccc ttgggccggg ccccctcggc gatcgcgatc agcgtgaagc gtttgccgtg
     2881 ccgcgagcgc tgcaggcact tttcacagac cgccgcaatg tcatagggga tttcgggaat
     2941 gaggatcacg tccgccccgg ccgccaaacc ggcatgcagc gccagccacc cggcattgcg
     3001 gcccatcaac tcgaccgcca tcacgcggtg gtgactggac gccgtcgagt ggatccggtc
     3061 cagcgcctgc gtggccgtct cgaccgccgt cagaaagccg aacgttatgt ccgagtgcat
     3121 caggtcatta tcgatggtct tcggcacgcc gatgcacggc acgccgtgcc ggatcatgtg
     3181 ggccgcgacg ctcatcgtgc cgtcgccccc gatgcagatc agcccgtcca ggtggcgctc
     3241 ctggatgtgc tcgaggacgc ggcgggtcac gtcctcgaaa atggggttcc cgtcggcgtc
     3301 gtggccggtt acgaagcggg ccgggttggc cttgttgctg gtgccgagga tggtgccgcc
     3361 gaccgttaga atgttggaga cgtcatccat ttccagcggg cgcatgcgat tgcgaatcag
     3421 gccgagaaag ccgtcctcga tgcccatgac ctgcaggccg agctgcaaaa tcgcggcttt
     3481 cgtgacgacg cggatgacgg cgttcaggcc gggacaatcg ccgccgccgg tgagcacgcc
     3541 gatacggctg agtttgtcag gcttgatgga catagcggct tccgtttacg ggagaaattt
     3601 agtttatcgg cggcggacat catgtccacc gcgggtggaa ctcaagggcg cgacattcat
     3661 cgctgaccag aatcattcat cgctgatcaa cactgcccag cggcgcgtgc tctcaatgtt
     3721 cgccagcgtg attttcagcg ccatcagcat gggcgcggcc agcaccaggc cgacggcgcc
     3781 ccacacccag ccccaatacg cgagcgaggc cagcaggaga accgggtcaa cgttgaggct
     3841 cttgcccgca aggcggatct cgatgtagtc gacccagacg aagcggttga tgatcagcag
     3901 cgcggccagg cccagggccg tccaggagtt gccgaattgc agaaaggcca gcacgaccgg
     3961 cggaacgagg gccgcgatgc tcccgacgta ggtgatgtaa ttgagcgcga acatcaacac
     4021 cgcccacaac ggccagaagt gcagcccgaa cagatacgcg atcagggcct ggcagatcgc
     4081 tatgcccagg ctgaccagcg tcttgacctt catgtattgg gcaatgccct tgttgatgtt
     4141 cgacccgatc tgcagcaggc gcccggccgt ttccggcgcg aaggcccgct gcacgcgtcc
     4201 gccgatgcgt cgcgcggaga taatgatgaa gaacaggtag aagaacacca ttacggccgt
     4261 ttcgaggaag ctgatggccg ggccgaacgc cagtccaagc acgtcgccgg tcgaaacgtc
     4321 gaagacttgc gctaccaggt ctttggcctg ggcccgcgcc tgtggtccgg gcagggcgtc
     4381 cagcaacccc agcaggttgg cgcgatattc cggcaaccgg ccgcggacga gcagcatgtt
     4441 gccgtgcaca attcgcccga gccagatgat cgcgacgacg gtcagtgtga aaaggaacag
     4501 atatgccagc caggccggcg ccccgcgtcg cacgagcgca tctgcggccg gacgaatgat
     4561 gtagtagaga aacagggcaa tcaggaccgg cgtaaggacc ggccccaggt acgtgatggc
     4621 cgccgcgacc agtaagaagg cgattacagc gaggccggcc gagcgaccgg tttctgagcc
     4681 tggacgcatg gaccccatgg tacccggcgt taggcgaccc cgccaacgcg tccgggcgca
     4741 cccgatgcaa gcccacccgg tccgagcccg cccggtagtc tcggccttcg tcctgtaaaa
     4801 atcgcctttg tggtgggtgt gaacggcggt ctcata
//
LOCUS       DAOSYS010000265         4830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14583_length_4830_cov_5.313508, whole genome shotgun sequence.
ACCESSION   DAOSYS010000265 DAOSYS010000000
VERSION     DAOSYS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14583_length_4830_cov_5.313508"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..598)
                     /locus_tag="PLU99_13495"
     CDS             complement(32..598)
                     /locus_tag="PLU99_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO18104.1"
                     /translation="MKIAVTATAPSLDSPLDPRFGRCPYFLLIDSDTLSFQAVESSSQ
                     ALGQGAGIQAAQLVANQGVMHVLTGHCGPNAHQALTAAGIGVTVGCGGTVRQAVEQFR
                     AGQLQTAGGPDVASHSGMTSPGTGAPAGADPSGSPSPLPNAPAAPSGGPGLGMGRGRG
                     GMGRGMGGGRIGRGGFGRRNCGGKAGGA"
     gene            complement(645..1037)
                     /locus_tag="PLU99_13500"
     CDS             complement(645..1037)
                     /locus_tag="PLU99_13500"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOO18105.1"
                     /translation="MPGGDHTGPLGKGAKTGRGAGYCAGFRMPGYMNPLPGRGRWAAD
                     RSGPASGGGRGWRRRFYATGLTGWQRGGGRGCRGRAFYRAADPPAPTREQQIAALRAD
                     LQCLESSAEQLRQRIAALEATQADTAST"
     gene            complement(1064..1513)
                     /locus_tag="PLU99_13505"
     CDS             complement(1064..1513)
                     /locus_tag="PLU99_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOO18106.1"
                     /translation="MAIPTLEGRVSPVFDVARCVIVVELDGEREVRRQTLALHSPDLT
                     RRVAALTEQAVDVLICGAVSRPLEALLSAAGVRVISQTCGPVEDVLRGFIAGRLSDGA
                     FLMPGCCVGRRQCRRGGPGLLRRLGGPGRRRTRCRNADGTGPKATGA"
     gene            complement(1762..2529)
                     /locus_tag="PLU99_13510"
     CDS             complement(1762..2529)
                     /locus_tag="PLU99_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HOO18107.1"
                     /translation="MTFDAHSPVAEDAVTATPGPRKRRVARILLAATVTYIFIAYLIL
                     PALWKRRELRHPALADGETVTHTADGIPGDPLNIALVASEEQIHRSMLAAGWYPADPT
                     TIRSALRIAADCVFKRPFDEAPVSALYLFGRKEDLAFEKPVGDIPRERHHVRFWRAGE
                     LDSQGRPLWFGAATFDVRVGFSHNTGQITHHIAPEVDLDRDLLIDDLSRAGVLSEKYW
                     IDNFHKVLEGRNGGGDPWRSDGRLAVGVVREFAACPH"
     gene            complement(2719..4152)
                     /locus_tag="PLU99_13515"
     CDS             complement(2719..4152)
                     /locus_tag="PLU99_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOO18108.1"
                     /translation="MRRRLSTALLLVLVNAALAHEGEQRTTVVIDTDMGLDDAVTLAI
                     ALQSPHVQIAAIVACEGAAGGQTAVTHLERMLELFNRPDIPLYSSVTIASSKPAPPFR
                     QFAEHAVRAALPAEAKPRRRPFSAEAYAQPAGLTVLALGPLTNLAAALQTTPAIKERI
                     GRVIIAGPADPQKNWNLSYDPQAWHAVRACGLKLEFVADGAPCKPQSWRQTAPAIGRN
                     ASLGANFIRHLLAEAGVREHYFSRWPGFSDELTFLYCTDPDLFARNGQGGVFIPRDAQ
                     ALFATFAHDVANGRQHKRRVVLNDLALPEEMLCADLRQRREAIIAKNGEIEWFLELLT
                     SELHDHLGAYSVIGAKMGLRAAELLNAPPHAMKVTSYSAAMPPVSCMNDGLIVASGST
                     PGRGLYKHEPGDAGSTRVQFEYNGRALVLSLKPEYSARIAADIGRLEREFGLARREYW
                     EGVRRVALEIWENWHRGEIFAVLPGEL"
     gene            complement(4263..>4830)
                     /locus_tag="PLU99_13520"
     CDS             complement(4263..>4830)
                     /locus_tag="PLU99_13520"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOO18109.1"
                     /translation="VDVRIVAATHRNLQEMVRAGRFREDLYYRVNVVRLRLPPLRERR
                     EDIPLLIERFITRFNRMQNKDIVGLSDGALAILMAHDYPGNVRELENIIEHAFVLCRA
                     GLIEPQHLPPALQAAGKVSRRFKTGLTLREVEAIHINDALRRHRGNRAAAARELGINA
                     STLFRKVQKLGGAAPGASAPPARSKRPG"
BASE COUNT          840 a         1660 c         1496 g          834 t
ORIGIN      
        1 cccattttca cctcggctat gcggcgttcg gctaggcgcc gccggctttg ccgccacagt
       61 ttcgcctgcc aaacccgcca cgtccaattc taccgccccc catgccgcgt cccattcctc
      121 cgcgacctct ccccatcccc aggccgggtc cgccactggg cgctgctggg gcattcggca
      181 ggggcgaagg cgagccggac ggatctgccc cagccggggc gcccgtgccg ggggaagtca
      241 tcccgctatg gctggcgaca tccggaccgc ccgccgtctg aagctgaccc gcccggaact
      301 gctcgaccgc ctggcgaacc gtcccgccgc aaccgacggt cacgccaatg cccgccgcgg
      361 tgagcgcctg gtgcgcgttc ggaccgcagt ggccggtcag cacatgcatg acgccttgat
      421 tggccaccag ttgtgcagcc tgaatgccgg caccttgccc gagagcttgg ctggaactct
      481 caaccgcttg gaagctcagc gtgtcactat cgatgagcag gaagtatggg cagcggccga
      541 accgcgggtc cagcggcgaa tccaggctcg gggccgtggc cgtaactgcg attttcatgc
      601 ttgacctctt tctactttct tgagtcagcc gagccgaagc aacgctacgt gctcgccgta
      661 tcggcttgtg tcgcctccag ggctgcgatg cgctgccgga gttgctcggc gctgctctcc
      721 aggcattgca ggtcggcccg cagggcagcg atctgctgct ctcgcgtcgg cgccggcggg
      781 tcggcggccc gatagaacgc tcgaccgcgg cagccgcggc ccccgccgcg ctgccaaccc
      841 gtcagaccgg tagcgtagaa gcggcgccgc cagccgcggc cgccaccact cgctggtccg
      901 ctgcgatccg cggcccagcg cccgcggccg ggaagggggt tcatgtaccc cggcattctg
      961 aaaccggcac aatagccggc gccgcgaccc gtctttgctc cttttccgag cggtcccgtg
     1021 tgatctccac caggcattgg tttctccttt gcatttccga gttctatgcc ccggtcgcct
     1081 ttggtcctgt tccatccgca tttctgcacc gcgtccgacg gcgccccgga ccacccaggc
     1141 ggcgaagcaa accgggcccg ccgcggcgac attgccgccg gccgacacaa caaccgggca
     1201 tcaaaaacgc gccatcgctc agccgccccg cgatgaaccc acgcagcacg tcttccaccg
     1261 gcccgcaggt ctgcgaaatc acgcgaactc cggccgcact cagcagcgct tccagtgggc
     1321 gcgacaccgc gccgcatatc agcacgtcca ccgcctgctc cgtgagtgcg gcgacgcggc
     1381 gcgtcagatc cggcgagtgc agcgccaacg tctggcggcg cacttcgcgc tcgccgtcca
     1441 gttcaacgac gatgacgcat cgcgccacgt cgaacaccgg cgagacgcgc ccttccaggg
     1501 ttggaatcgc cacgatcatg ccctgctatt ggcagcccgc atgccaggcg gtcttcgacc
     1561 ggcagaagcg ctcataacac gctacgtttt aagggtttac ggccgagatg ccggagttga
     1621 acgcccgcga gacacaattg catgttgcaa tcatccgctg taccaggtat tgcaaaatgc
     1681 aacgccgacg ggcgcgtccc tgcgccagcg gggctcgttc ttgtatggtc tggactttgg
     1741 cggggttact tctcaacccg gtcagtgcgg gcaggccgca aactccctga cgacgcccac
     1801 tgccagccgg ccatcgctgc gccagggatc gcctccgccg tttcggcctt ccaacacctt
     1861 gtgaaagtta tcgatccagt acttctctga cagcacgccc gccctgctca ggtcatcgat
     1921 caacaggtcc cgatcgaggt ccacctcggg agcaatatgg tgagtgatct gcccggtgtt
     1981 gtggctgaag ccgacgcgaa catcgaacgt cgccgcccca aaccacaacg gccggccttg
     2041 tgagtccagc tcgccggcgc gccagaagcg aacgtgatgt cgctcacgcg ggatgtcacc
     2101 tacgggcttt tcgaacgcca ggtcctcttt gcgtccaaac agatacagcg cgctcaccgg
     2161 ggcctcgtca aagggccgct tgaacacgca gtccgcggcg atgcgcagcg cgctgcgaat
     2221 ggtggttggg tcggccggat accagccggc cgccagcatg ctccggtgga tttgctcctc
     2281 actcgcgacc agggcgatat tgagcggatc gccgggaatc ccgtcggccg tgtgcgttac
     2341 ggtctcccca tctgcgaggg ctggatgccg cagctcgcgc cttttccaca gcgccggcag
     2401 gatgaggtag gcaatgaaga tgtacgtgac ggtggcggcc aacaggatgc gcgcaacgcg
     2461 ccgcttccgc ggcccaggcg ttgctgtgac cgcgtcctct gcaaccggcg aatgagcatc
     2521 gaacgtcacg gaatccctgc cttagtccgc actacgcttc cgcgttgatt ttagacggtc
     2581 ccccggtgtt gaacttaccg gcaggtgttt ctggcggcca tgcgcaagct gtaagcgcgg
     2641 gcatgggacg caccgataac atgcatccgt tgggtagtgt gcatcagctg cgatacttct
     2701 gacgtcgccc ttccgacttc acagctcgcc cggaagcacc gcaaaaatct ccccccgatg
     2761 ccaattttcc caaatctcca acgctacgcg gcgaacgccc tcccaatact cgcgccgggc
     2821 caggccgaat tcacgctcca gccgcccgat gtccgcggcg atccgggcgc tgtattccgg
     2881 cttcagcgaa agtacgagcg ctcgcccgtt gtactcaaat tggaccctgg tcgatcccgc
     2941 gtcccccggc tcgtgtttat agagcccgcg gcccggcgtc gagccactgg cgacgatcaa
     3001 cccatcgttc atgcagctca ccggcggcat cgcggcgctg tacgaggtga ctttcatggc
     3061 gtgcggcggc gcgttgagca gctcggccgc gcgcaggccc atcttcgccc caattacgga
     3121 ataggcgccc aggtggtcgt gcagctcgct cgtcaggagt tccaaaaacc attcgatttc
     3181 gccgttcttg gcgatgatcg cctcgcgccg ctggcgcaga tcggcgcaca acatttcctc
     3241 cggcagcgcc aggtcgttga gcacgacacg gcgcttgtgc tggcggccat tagctacatc
     3301 gtgggcaaac gtggcgaaaa gcgcctgcgc gtcgcgcggg atgaaaacac cgccttggcc
     3361 gttgcgcgcg aagagatcgg ggtccgtgca atacaggaac gtcaactcat cggagaagcc
     3421 cggccagcgc gagaagtaat gttcacgcac gccggcttcg gccaacagat gccggatgaa
     3481 gttcgcgccg agcgacgcgt tccgcccgat ggccggtgca gtttggcgcc aggactgcgg
     3541 cttacacggc gcaccgtcgg caacgaactc gagctttaga ccacaggcgc gaaccgcgtg
     3601 ccacgcctgc gggtcatagc tgagattcca gttcttctgc gggtccgccg ggccggcgat
     3661 gatcacccgg ccgatgcgct ccttgatagc aggcgtggtc tgcaatgccg cggcaaggtt
     3721 cgtcagcggc ccgagcgcca ggacagtcaa gcccgccggc tgagcatagg cttctgcgga
     3781 gaacggccgg cggcgcggct tcgcttcagc cggcagcgcg gcgcggacgg cgtgctctgc
     3841 gaactggcgg aatggcggag ccggcttgga ggaagcgatt gtcacggaag aataaagcgg
     3901 aatgtctggg cggttgaaca gctcgagcat gcgctccagg tgcgtcacgg ctgtctggcc
     3961 gccggccgcg ccttcgcagg caacaatcgc cgcgatctgg acatgcgggc tttgcagcgc
     4021 gatcgccagc gtgacggcgt cgtccagccc catatcggtg tcgatcacga cggtcgtgcg
     4081 ctgttcgccc tcgtgtgcca gggcagcgtt gacgagaacc aggagcagag cagtcgacag
     4141 gcgacgacgc atcatcaacc tccggacaac agggactccg ccgcgccggc ggaacacaac
     4201 cagagaacat aacccgccgg tattgtgaaa gaaacgagtg ttatggcgac ggagttcgat
     4261 cttcagccgg ggcgcttgga gcgcgctggc ggcgccgagg cgcccggcgc ggcgccaccg
     4321 agtttctgca ccttgcggaa cagcgtgctg gcgttgatcc ccaactcgcg ggcggctgcg
     4381 gcgcgattcc cgcgatggcg ccgcagggcg tcattgatgt gaatcgcctc cacttcgcgc
     4441 agcgtcaaac ccgtcttgaa acgccgcgaa accttgccgg cagcttgtag ggcgggcggc
     4501 aaatgctgcg gctcaatcag tccggcgcgg catagcacga acgcgtgctc gatgatgttc
     4561 tccaactcgc gcacattacc cggatagtca tgagccatga gaatcgccaa cgccccgtcc
     4621 gacagaccca cgatgtcctt gttctgcatg cgattgaaac gcgtgatgaa ccgctcgatc
     4681 agcagcggaa tgtcctcgcg ccgctcgcgc aagggcggca ggcgcaggcg gaccacgttg
     4741 acacggtagt acaaatcctc gcggaaccgc cccgcccgca ccatctcttg caggttgcga
     4801 tgcgtggccg ccacaatccg cacgtcgacc
//
LOCUS       DAOSYS010000266         4783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14752_length_4783_cov_5.086717, whole genome shotgun sequence.
ACCESSION   DAOSYS010000266 DAOSYS010000000
VERSION     DAOSYS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14752_length_4783_cov_5.086717"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PLU99_13525"
     CDS             <1..723
                     /locus_tag="PLU99_13525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOO18110.1"
                     /translation="ATVDGGAGCLQALGWRLLDAGGRELSPPVKGADLQRIARLEPPA
                     NPPVIDLQILCDVDNPLLGPRGAAPVFGPQKGASPDEVQQLAAGLESWAGVLKEATGR
                     EVADVPGAGAAGGLPAGLFCTLAARLAAGFDVLAEAVDLRNKLSGCDWCLTGEGRLDE
                     QTFGGKVVAGVTRCAAEAGVPTAAFVGAAVLRARETLEQLARRLGTAAIRVISPPGVP
                     HEAALAQTVSNLRREVADFVRA"
     gene            856..4302
                     /gene="ccsA"
                     /locus_tag="PLU99_13530"
     CDS             856..4302
                     /gene="ccsA"
                     /locus_tag="PLU99_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOO18111.1"
                     /translation="MTEEIGLAQSRRPRSGIVAFLGSTWTGIASLVLILLYASIGSGL
                     APVRGALEMTEMQVFEHWFFVALVAFFLISLVVSTLTRIKFSWMSAGALMAHAGLVLL
                     TVGAFRYFAYKVEGDVLLMSPRVELCTVDGKRLDVAFLAEKGQTWEEIVPVLGGVVRF
                     EVLDTQPAANVPVASATVKASIGDAPPVTVALGMADRSATPVGGGLALQLRTFAPQTK
                     FYDKEVAALYFGPTGGELQFTPIPGLPLHRERYTPDEGELYDVLGRPVPSKRTSPHLN
                     LAGLKIPTGWFETWRMPIKLDTAGLPFDVEVTGYVPYVAGTAAKPVIQPLQLRRPGQV
                     REPSAIRLKLTGRGAYEGWSESRWCYFAVYPRVDANAAAVIGAPPKQTVLLPDGKSYD
                     LLYSRLEHDLGASLIPLKLSVKHMPGGRMVESWRSDFLVQRGQAPPTPAAVFTNQTYS
                     VAPWTLFQANAAGDGWTFTGLGVGNRRGMLLMLLGSVLTTLGCVYAFYIKPIIKQRRR
                     AWGDEGSRDQGIEGMKGEQSREVAPRSAPLTANSPRVAPLLMLLAFPFLAASAQGAEP
                     FAASQNAVALDRQIDWRHAKLIVVQDAGRYKTLEAFARERVSAMYGTGHLPGLSPCAS
                     LFELLFNGEQYDDVPLVKVRERDLRIDLTEHMAEVERLRVRQSGYLTPRELRAPAVQK
                     RIRELLPINTKTRALNRMLDAQATARDLKDMMLIVPRPNAAREAAWFTPDELRTNAAL
                     AVPEGQRLSAEELARRFGPPAFGISPTEAARALEPWLRLQEAWQRADAPAVQTALANL
                     TEVLPALAAPGVYPPEAQRQAEAHYYETFTSNTIFSRAFLGPLAYLLGGVLAVIAFVT
                     RWPNPRRISLLLMALGLIWHAYMVGLRWYVLGRIPVANMFEAITAAAWGGIAIALLAE
                     LFYRTGVFLIAGNAAGFSALTVASFVLPGGGAITSMMGILDNIMLRLHTVMIVLAYAL
                     IFIGAVIAVIYLLGYYYYDRLRHAEPLTAAREARLGRAVALPQREVPVTGGLQPAPIL
                     AGAAPSDIGLGRSLPGWLHNTDWSHLIVLNMAFILLFVGGVVLGAVWADFSWGRPWGW
                     DPKETFALNTWLIYAVLLHVRYVVRNKGLWTAWLSIAGCAMMLFNWFVVNYYIVGLHS
                     YA"
BASE COUNT          787 a         1526 c         1562 g          908 t
ORIGIN      
        1 gcgacggtcg acggcggcgc cggctgcctt caggccctcg gctggcggtt gctcgatgcc
       61 ggcgggcgtg agctgtcgcc gccggtcaag ggcgcggatt tgcagcgcat cgcgcgcttg
      121 gaaccgccgg caaatccgcc agttatcgat ctgcagattt tgtgcgacgt ggataacccc
      181 ttgctcggtc cacgcggcgc tgcgcccgtt ttcggcccgc agaaaggggc ttcgccggac
      241 gaagttcagc agcttgcggc cggactcgaa tcgtgggccg gtgtgctcaa agaggcgacc
      301 gggcgcgagg tcgccgacgt cccaggcgcg ggcgcggccg gcgggttgcc ggccggattg
      361 ttctgcaccc tggcggcgcg gctggcggcg ggtttcgatg tcctggcgga ggctgttgat
      421 ctgcgcaata agctctccgg ctgcgattgg tgcctcaccg gcgaaggccg actagacgag
      481 caaacgttcg gcggcaaggt cgtcgccggg gtgacccgct gcgcggcaga agccggcgtt
      541 ccgacggccg cgtttgtcgg ggccgcagtc ctgcgagcga gagagacgct ggagcaactc
      601 gccaggcggc tcggcacggc cgcgatccgg gtcatcagcc ctccgggcgt gccacacgaa
      661 gcggcgttgg cccagactgt gagcaacctg cgacgcgaag tggcggattt cgtgcgggcg
      721 tgaggcttgg gtatgcgcga gctcgcggac tttggcagca gcgccgcggc tgacctcgcc
      781 cgaaaagccc gccgccctgt ataattcggg caagggcgta gggccgctct gctcgcaaaa
      841 attggaagca tccgcatgac cgaagaaatc ggccttgctc aaagtcgccg gccgcgcagc
      901 ggtatcgtgg cgtttcttgg cagcacgtgg accggcatcg ccagtctggt gctcattctg
      961 ctctacgctt cgatcggctc gggtttggcg ccggtgcgcg gcgcgctcga gatgaccgag
     1021 atgcaggttt tcgagcattg gttctttgta gctttagttg ctttcttcct gatttcgctg
     1081 gtcgtctcga cgctcacgcg catcaagttc agttggatga gcgccggcgc gctcatggcg
     1141 cacgccggcc ttgtgctgct gaccgtcggc gcatttcgct acttcgcata caaagtcgag
     1201 ggcgacgtgt tgctgatgtc gccgcgcgtc gaattgtgta cggtcgacgg caagaggctg
     1261 gacgtcgctt tcctggcgga gaaggggcag acttgggaag agatcgtccc cgtcctgggc
     1321 ggcgtggtgc gtttcgaggt gctcgacacc cagccggcgg cgaacgtgcc ggtggcgagc
     1381 gccacggtta aggccagcat tggcgatgct ccaccggtga ccgtcgcact cggtatggcg
     1441 gacagaagcg cgacgcctgt cggcggtggg ctggcattgc aactgcgaac ttttgcgccg
     1501 cagaccaagt tctatgacaa ggaagtcgcg gcgctatact ttgggcctac cggcggggag
     1561 ctgcagttca cgccgatccc cgggctgccg ctgcatcgcg agcgctacac gcccgacgag
     1621 ggcgagctgt acgacgtgtt gggccgcccc gtccccagca aacgcaccag tccgcacctt
     1681 aaccttgccg gcctcaaaat cccgaccgga tggttcgaaa cttggcgcat gccgatcaag
     1741 ctggacaccg ccgggctgcc gttcgatgtc gaggtgaccg gctatgtacc ttacgtcgcc
     1801 ggcacggcgg ccaaaccggt gattcagccg cttcagttgc ggcggccggg ccaggtgcgc
     1861 gagccgtcgg cgattcgtct gaagctgacg ggtcgcggcg catacgaagg ttggagcgag
     1921 tcgcgctggt gctactttgc cgtgtatccg cgcgtggacg cgaacgctgc ggcggtaatc
     1981 ggggcgccgc caaagcagac cgtgctgctg cccgacggca agagctacga tctgctctac
     2041 tcgcggctgg agcacgacct cggggcgagc ctcatacccc tcaagctgag tgtgaaacac
     2101 atgccgggtg ggcggatggt cgaaagctgg cgcagcgact ttctcgtgca gcgcggccag
     2161 gcaccgccga cgcccgccgc ggtgtttacc aaccaaactt actcggtcgc cccctggacc
     2221 ctgttccagg ccaatgcggc cggtgatggc tggactttca ccgggctggg tgtgggtaac
     2281 cgccgcggca tgctgctgat gctgcttggt tcggtgctca ctactttggg ctgcgtttac
     2341 gcgttttaca tcaagccgat catcaagcag cggcggcgcg cttgggggga tgagggatcg
     2401 agggatcaag gtatcgaggg aatgaaaggg gagcagagcc gggaggtggc gccgcggagt
     2461 gcgccgctga cggccaattc cccacgtgtt gccccgctgc tgatgctgct ggccttcccg
     2521 tttttggccg cgtccgccca gggcgcggag cccttcgccg cttcgcaaaa cgccgtcgcg
     2581 cttgaccgcc aaatcgattg gcggcacgcc aagctgattg tcgtacagga cgcggggcgc
     2641 tacaaaacgc ttgaagcctt cgcccgcgag cgcgtgagcg ccatgtacgg cacggggcac
     2701 ttacccggcc taagcccgtg cgcgtcgctg tttgaattgc tgttcaacgg cgaacagtac
     2761 gacgatgtgc cgctcgtcaa agtccgcgaa cgcgacctgc ggatcgacct gaccgagcac
     2821 atggcagagg tcgaacgcct gcgcgttagg caatccggct acctcacgcc gcgcgaactg
     2881 cgtgccccgg ctgtgcagaa acgtatccgc gaactgctgc ccattaacac caaaacccgc
     2941 gccctcaacc gcatgcttga tgcgcaagcc acggcgcgcg acctgaaaga catgatgctc
     3001 atcgtgccgc gcccgaacgc ggcgcgcgaa gccgcctggt tcaccccgga cgagctgcgc
     3061 actaatgccg ccctggcagt gccggagggg cagcgcctca gcgcggagga actggcgcgg
     3121 cgcttcggcc cgccggcgtt cggcatcagc cccactgagg ccgcacgggc cctcgagccc
     3181 tggctccgcc ttcaggaggc ctggcaacgg gccgacgctc cggccgtcca aacggcgtta
     3241 gctaatctaa ccgaggtcct cccggcgctg gccgcgcccg gtgtttatcc gccagaggca
     3301 cagcgccagg ccgaagccca ttactacgag acgtttacca gcaacaccat cttctcgcgg
     3361 gccttcctgg ggccgctggc ttacctgttg ggcggcgtct tagccgtcat cgccttcgtc
     3421 acgcgttggc caaacccccg ccgcatctcg ctgctgctga tggcgttggg actgatctgg
     3481 cacgcctaca tggtcggcct gcgctggtac gtccttggcc gcattccggt ggccaacatg
     3541 ttcgaggcga tcacagcggc ggcctggggc ggcattgcca ttgccctgct tgcagaactg
     3601 ttctatcgca ccggtgtttt cctgattgcc ggaaacgcgg ccggcttttc cgcgctgacg
     3661 gttgcgagct tcgtcctgcc cggcggcggg gcgattacgt cgatgatggg cattctcgac
     3721 aacattatgc tgcgcctgca cacggtgatg atcgtgctcg cgtacgcctt gatcttcatc
     3781 ggcgcggtga tcgcggtcat ctacctgctc ggctactact actacgatcg cttgcggcac
     3841 gccgaaccgc tgaccgctgc gcgcgaggcg cggctcggca gggccgtcgc gctgccacag
     3901 cgcgaagtgc cggtcactgg cggcctgcag cctgctccaa ttctggccgg cgcggcaccg
     3961 agcgacatcg gtctggggcg atcgctgccc ggctggctcc acaacactga ctggagccat
     4021 ctgatcgtct tgaacatggc cttcattctg ctcttcgtgg gcggcgtggt gttgggcgcg
     4081 gtgtgggccg acttttcctg gggccggccg tggggctggg atcccaagga aaccttcgcc
     4141 ctgaacacct ggctcattta cgccgtgctg ctgcacgtgc gctacgtggt gcggaacaaa
     4201 ggtttgtgga cggcctggct ctcaatcgcc ggctgcgcaa tgatgctctt caactggttc
     4261 gtggtgaact actacatcgt cggactacac agctacgcgt agcagggtga taaggtgata
     4321 aggtgataga gtgataaggg agtaagcgcg aatacgccct ccttatcact ctatcaccta
     4381 atcactttat cacttcacgg atacccgtcg cgcagcgctg caatgcagac tgctgaatcc
     4441 accttaagcc tctcgccgcc gcgcccccca aaaacaccgc tgggcgccgg cggcctacaa
     4501 ccgccagcgc ccagcttgtg agaagattgc gtccgccgct tacttagcgc agcggctacc
     4561 gcaaccggcg cgcgttttct tcgcggccgg tttcttggcg gtcttcttct tcggagcttt
     4621 ctttgcggct ttcttagttg ccatggcgct tacctccaag aaacagaaac ctcagacacg
     4681 agacacgatt cgacaatcgt gacaacggca tagcgaatcg aattaccggc ggttcgtcaa
     4741 gagcgcgcga ccattaataa cgcacactcg tccagaaaat cga
//
LOCUS       DAOSYS010000267         4776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_14782_length_4776_cov_4.599026, whole genome shotgun sequence.
ACCESSION   DAOSYS010000267 DAOSYS010000000
VERSION     DAOSYS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14782_length_4776_cov_4.599026"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            660..833
                     /locus_tag="PLU99_13535"
     CDS             660..833
                     /locus_tag="PLU99_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18112.1"
                     /translation="MMLFRLRGVALTGLLILAVLCMGGACQQKVAFSFTETFLNQLAL
                     NLAQTVVDKALGD"
     assembly_gap    1003..1012
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1288..3207)
                     /locus_tag="PLU99_13540"
     CDS             complement(1288..3207)
                     /locus_tag="PLU99_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18113.1"
                     /translation="MKLPLIVGAGIAALVLAAFIVSPQLRSEAAKTAQQARQQATLAE
                     RALHRYNYRLPTVGRLAPPEELKTADAADLAEMAKPGIEEGKKDLSKRLNEIRSQAQK
                     AGLPPPKTDLPTADASGLKRSLGQYDQMLADNEQLLKQAAQEARAAKQAGSSVLGVSQ
                     VAGMVEYTRASRALAEASALRDAQQHLQAELRLANSQARNYEGSRDCAMGMEMAAVVS
                     GLQNDLAEVEKLAAAATARAAELAARVSQREQELAPIKAEQDQVRDELLALENKGFTA
                     GDDESFASYRAQYERLSTRARELQEQEELLRLGGLRGATFAGDDYQNAEIEGGEQIVS
                     LEMLREAAAAAQEAAMRGTQAVEELRKQIKTAEQSGRDAQAAVERYTALVASLEARQA
                     ELWPQIEDLAEKAGAKEDEALKAASDAASAFRSSQQAADGWLREARDLQSERDPERVN
                     PRLKMMLDDKGIPSMGSSAEAEALVLVGRIYAQRIEANRALLADVRQLRDGGEEAQAA
                     TQKLQEQIESDMGEATEKLQSAIKLYEGLASKVKPETKWVPQAALAGVYTLLARINPA
                     EAAAHMSDALARIQESIGARERSPYVQKYVEFQEHIRQALESGEQGAAAEKPAKQEPG
                     AAEAPAEEEPAGEGD"
     gene            3721..3794
                     /locus_tag="PLU99_13545"
     tRNA            3721..3794
                     /locus_tag="PLU99_13545"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3755..3757,aa:Cys,seq:gca)
     gene            4189..4461
                     /locus_tag="PLU99_13550"
     CDS             4189..4461
                     /locus_tag="PLU99_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18114.1"
                     /translation="MLNPKTYKSPSGEYELHVDPSTKYGEGEGHYRMLRGGAEVWAKK
                     LPFTLWDAGVADDGTAAGYAEAEGADTVHLVIVADIGYRSRAVQVV"
BASE COUNT          736 a         1566 c         1513 g          951 t
ORIGIN      
        1 taactcgctg cgcgagagca ggagagaggt gaacaggagg cgggcgatct gatgctcatg
       61 cgggctcatg cggggccttt ctgcgcgcgg gcgcgcggtt tctgctagac attcaataaa
      121 gccatccgct ttcagctctc agctttcagc cagaatgcgc ggattgcggg tgtatgcgcg
      181 cgagtggcgg ggaaacgctg cttggggtga taaagtgatg acgtgatagg gcgataagga
      241 aagctttcag ctgtcagctt tctcttcgga accgccaaga cgccaaggcc gccaattgcg
      301 gattatccgc gccagtctcg ctgctcgcgt gatgcggtaa aggcgaaacc tttcggcttc
      361 agctttcagc tctcagcgaa ggcaaccgca tcggcgcaaa aactagccgc gcccccgtta
      421 cgaccgccgc ttgttcaggg actaactgcg tccccgtttc acaccgccaa caaacggcgc
      481 taagaaacta gctgcgtccc cgtttcacgc cctcggtcgc ggcacgcccc ggctcagctc
      541 tggcgcaaga cggtggcgtc gcgcaacgtt atacttcgcg ttccgcgggc ggcggccgcg
      601 gcgtgcgatg ggaaggaccg gccgccggaa acttcaggat tcccgcttgg aggcattcaa
      661 tgatgctgtt tcgtttgcgg ggtgtggccc tcaccggatt gcttatcctg gccgtgctct
      721 gcatgggggg cgcctgccag cagaaggttg cgttctcgtt cacggaaacg ttcctgaacc
      781 agttggcctt gaacctggca cagaccgtcg tcgataaggc actgggcgac tgacgggctg
      841 tccgcttacg gcccgcggct ccggcccggt cagcagcgca acgaatgggc cgatctcgaa
      901 gacgttaagt gcgccgtttt ggttaatgtc cgcgttcgtc aaatacaggc cgggaacgcc
      961 atcgcgtacc cggccgggtc ggttaacgcc aacacgaacg cgnnnnnnnn nnggggcgat
     1021 gtcgaagagg tttacttcgc cgtcttcgct aatgtcagcg ttcgtcaatt cggaagccgg
     1081 gaacgccatc gcgtacccgg ccgggtcggt taacgccaac acgaacgcgt cggtcacgaa
     1141 gacattgacg gcgtctggcg accccggcgc atacgcccct ggcgcattcg agcgaacgag
     1201 tcgctgcggt ctcgtttagt aatggaagcg ccgcgcggct gttcacgctg cgcggagcga
     1261 ctcacctgct tggcgtctca gaattgtcta atcgccttcg ccggccggtt cttcttccgc
     1321 cggcgcctcg gctgccccag gctcctgttt tgcgggcttt tcggcggccg cgccctgttc
     1381 gccggactcc agggcctgcc tgatgtgctc ctggaattcg acgtacttct ggacgtaagg
     1441 gcttcgttcg cgcgcgccga tggattcctg aatcctggct agcgcatcac tcatgtgggc
     1501 cgccgcctcc gcaggattga tacgcgccag gagcgtgtag acccccgcca gggccgcctg
     1561 cggcacccac ttggtttccg gtttgacctt gctcgccagc ccttcgtaaa gcttgatggc
     1621 gctctgaagc ttttcggtcg cctcgcccat gtcgctttcg atctgctctt ggagcttctg
     1681 ggtcgcggcc tgcgcctcct cgccgccgtc ccgcaactgg cgcacgtcgg cgagcagcgc
     1741 ccggttggcc tcgatccgct gcgcgtaaat ccggccaacc agcaccaagg cttcggcttc
     1801 ggcgctgctg cccatggatg gaatgccctt gtcgtcgagc atcatcttca gccgcgggtt
     1861 gacgcgctcc gggtcacgct ccgactgtag gtcgcgcgcc tcgcgcagcc agccgtccgc
     1921 cgcctgttgc gaactgcgaa aagcactggc cgcgtcgctc gcggctttca gcgcctcatc
     1981 ctctttcgcg ccggcctttt cggccaaatc ctcaatttgc ggccaaagct cggcctgccg
     2041 cgcttccaag ctcgccacca acgccgtata gcgctcgacc gccgcctggg cgtcccgccc
     2101 gctctgctcc gcggtcttga tctgtttccg caattcctcc acggcctggg tgccacgcat
     2161 agccgcttcc tgggcggccg cggccgcttc gcgcagcatt tccaggctca cgatctgctc
     2221 accgccctcg atctccgcgt tctggtagtc gtcaccggcg aaggtggctc cgcgcaagcc
     2281 gcccaagcgc agcagttcct cctgctcctg aagctcgcgg gcccgcgtcg acaagcgctc
     2341 gtactgtgcg cggtagctcg cgaaagactc gtcgtcgccg gcggtgaagc ccttgttctc
     2401 cagggccagc aactcgtcgc gtacctggtc ctgctccgcc ttgatggggg ccaactcctg
     2461 ctcgcgctgg ctgacccgcg cggccagctc cgccgcgcgc gcggtggccg ccgcggcaag
     2521 tttctcaacc tcggccagat cattctgcag cccgctgacc acggccgcca tttccatccc
     2581 catggcgcag tctcgcgaac cctcatagtt ccgagcctgt gagttggcca gccgcagctc
     2641 ggcttgcaga tgttgctgcg cgtcgcggag cgcactcgcc tcggccaagg cccgcgacgc
     2701 ccgcgtgtat tcgaccatgc cggccacctg cgacacgccc agcacgctcg aaccggcttg
     2761 cttggcggcg cgggcctcct gcgcggcctg cttcagcagt tgctcattat cggccagcat
     2821 ttgatcgtac tgcccgagcg aacgcttcaa accgctcgcg tcggccgtcg ggagatctgt
     2881 tttgggcggc ggcagcccgg ccttctgagc ctgcgaccga atctcgttca gccgcttgct
     2941 caggtccttc ttgccctcct caatgcccgg cttggccatc tccgccaaat ccgccgcatc
     3001 ggccgtcttc aactcctcgg gcggtgccag gcggccgacg gtgggcaggc gatagttgta
     3061 ccggtgaaga gcacgctcgg ccaacgttgc ctgttggcga gcctgctggg ccgttttggc
     3121 cgcttctgaa cgtagttgcg gggagacgat aaacgcggct aaaacgagag ccgctattcc
     3181 ggccccgacg attaagggca gtttcacaat gcgctccatc ttgccggcct ggtcggctcg
     3241 gccatcccgc gtgcccgccc ggggcgggac cagggctgtc cccctaccat tacctgtgta
     3301 acctgtgctc aagtgactgt caatcgcgag cttatgttgt cggttgaacc ggcccggcgc
     3361 cggacagccc gccgaggcgg cagcggcgga gacggcgggc ccgaacgggc ctgcacgcgg
     3421 cgccgcgggg tgggagcata catcgctgcc gtgcgtagcg ctgcgggttc ggacgggaag
     3481 acgccagagg gggcggtcga gcgcggggat gggaggttgt tggcggcaat cccgggcgac
     3541 acgccccggc tctgctcgca caaaggcgat ttgcgacgcg ttggcatcat tccggggcga
     3601 cacggcccgg ctctgctctc agcccgatat tcgcttgccg cggtgccgct atgagtaccg
     3661 aaatgccgat tcgatattca cttgccgggt tgccgatatg tggtacgata cggggcgtgt
     3721 ggtggcgtgc ccgagtggac taagggagcg gattgcaaat ccgtttttca tgggttcgaa
     3781 tcccatcgcc acctttcccc ccttctgccg cgctgttcgc gcctgatcgc gtatctccgt
     3841 tgctgaaaac gagttacgcg ccgcgtccgc agcactgaca cttgaagcgc gggtgcgccc
     3901 tggcgcgggt caccgcctgc atctgaccca cgcgaccgcc ttgagcgtcc tcggggactg
     3961 aagccgtagc gagatcgtcc cggcgccctg ttccgcgcgt aggtgtagaa tggtgcgcgc
     4021 ctcgcaagct ggacgaatgt gccgcggccg gtgcgacact tggcgggcga ggcgcgcgcc
     4081 acaccggagg taaactgtga agcacgtcat cacgcacacc gttctggttc tgatcgtcct
     4141 gggtttggac gcccccgttc gcacgcttgc cgcattggcg gaagacctgt gctgaaccct
     4201 aagacttaca agtcgccctc gggcgagtac gagctgcatg tcgacccgag cacgaaatac
     4261 ggcgagggtg agggccatta ccgcatgctg cgcggcgggg cggaggtctg ggccaaaaaa
     4321 cttcccttca cactctggga cgccggcgtt gcggacgacg ggacggccgc gggttacgcc
     4381 gaagccgaag gagccgacac ggtgcacctc gtcattgtgg ctgatattgg ataccgcagc
     4441 cgagccgtcc aagtggtatg agatgagatg tttcctgcct ttatgcggat ttctggcgcc
     4501 atttctttgt ggaggacggg caggagtttt ggatgctggc tgaagggacg cgcgaggtgg
     4561 agcggtttgc cctgccggct gagttgcctc agtgactgaa tccgagcgcg cgggtggcgt
     4621 tggctgggag tgcgcgcgag cgtgagtgat gttggcggca ttccggggcg acacgccccg
     4681 gctctgatac cgaccgacat ccgacggctc tgctttagtc atcctccgcc gccgcacgtt
     4741 gcacgtgaat atgaacgcga ttgatcttcc gcgact
//
LOCUS       DAOSYS010000268         4699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15052_length_4699_cov_3.485142, whole genome shotgun sequence.
ACCESSION   DAOSYS010000268 DAOSYS010000000
VERSION     DAOSYS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15052_length_4699_cov_3.485142"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PLU99_13555"
     CDS             <1..1832
                     /locus_tag="PLU99_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18115.1"
                     /translation="PRESLEGVFSGVAFKRIPLLGLVTAVLGSFAVAAAGEPERGAAP
                     PEFAIRAASGVFRAYQPEAGQTVIDEYVRARVDQVGLDTWRLDLTNTGNQPIEAVWFP
                     WESSLQAVGQSLGDDVTYYPKQLGVAAKTDTLPPDEWIGQPYPGTCGPLIVQADRWAA
                     RMIAAVNWPPRILSPLMARGRMALRYDQPLAPQEQRCYTALCCEARGDEARGEIPWHK
                     VLDRYKEWLLENMRREGLYPIAYPEPMWRANGWVNVQLQNIPRFDVHELWEAYQKARD
                     VLPWVQFWGQMSNYHRCPAEGKKDWPDPPLEPGEKTGCCLPLARFHRRYMPDVAHFAR
                     EVCASGGIVGYYLRPWPPQEYLAEGKDSLAFLLNWIEVSREGGACAFYLDTLGTRPFG
                     DPLLVARLFGDRLPAMSVIEWPVDIYPTAFLASGALWGGLDWSTEPGRTPADLSVTLQ
                     RVTFPAFGRYLLDDRVFFLGASNGDYMWWANYRGHQHWTERQAFLLGMKFDAPAHLPK
                     HAAGWINPANEVVRAVADQWNKVGWWERQPRYRDRDGIYEVSPGIDVRRFEDRDGVTM
                     FVLDNWMRRGEAAFTYRGRRIIGPPQQLCVMILPRELEPQHAQ"
     gene            1849..3213
                     /locus_tag="PLU99_13560"
     CDS             1849..3213
                     /locus_tag="PLU99_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HOO18116.1"
                     /translation="MYLVLHILITLVCGTICLLAARRLQAKLPKMIMFSALGLIHGVV
                     PALTPRMFMWPGAAQANIDYAALLALLGILAFALGWRIMERSADPQGLAPGLYYYLYS
                     SEGQAALKRLFWATAVLGCAAWLGCMIVSAGSVSAAFSAARFQYRMRGNIYLTTLLTH
                     VSSLMAVPCFTCFFLPRRYRMLGASFGISMALLLFVASQGARAAALGMIGATGLGYSF
                     AHRLRLRRALLVGAVGGAILLLAVATYEVRKVMTRRPASEVLRMLVSPETYQGALTRD
                     PLYYHQFLVAAVMHFPDLHPYLNGAAYQRILFFYLPHRYFPELKPPDTHNTFARVITG
                     SSTRTTIPPTMIGDGYINFWGLPGVIGIMLFHGMLFGWAERKIRERLLVFLAIGASFA
                     NCALLAVRGSPYEVLTLCLSASGILWVLGSACGLRFRKARRSPPALPSVTLVSTALSS
                     QPQI"
     gene            3210..4595
                     /locus_tag="PLU99_13565"
     CDS             3210..4595
                     /locus_tag="PLU99_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18117.1"
                     /translation="MSRLAEHGAKLRASLRRLPNTRVRGETTWVIAHKAAEFCVMLAG
                     LKLFTNLMSQASFGEYQLGLTTLALITHLFETPISQVYLRQYHTALSQGTARSALSQT
                     MRWLGATTIVVAGGCALLTKPLADAFELETLTVLAVGLIFLTNRWRSLRVQVLDVQRK
                     RRQCMFENLGFFALQTTLGAAALYFIRPSATTALLSYALAAGVFGSVALRGIRRDLRT
                     LPAGKPAPFAGLVYAYGVPLGLLTTFQWLQSFGERYVLCSQLDYATVGRYVAAYQVCG
                     FPFMAMNAVLTALVQPVAFQRASDISDPRQVWSADRLLLAGIGLYVAAGVLIVAGYAF
                     FGPLLMQVLASDEYVLPASSFTILAAARMLMFASLLQHVFFKVHQQTRFLLFYSVIGG
                     VILLPAAWLLVSAYGMMGAAVAVLTTAVIYTLLLAFAPGGIWWLLRGVRTRAAAVPSV
                     STPGAATEGCL"
BASE COUNT          761 a         1387 c         1542 g         1009 t
ORIGIN      
        1 gcccgcgtga gtcgctggaa ggggtcttct cgggagtggc cttcaagcgt attccgttgc
       61 ttgggctcgt cactgcggtg ctgggcagct ttgcggtggc agccgcgggc gagccagaaa
      121 ggggcgcagc gccgcctgag ttcgccattc gtgcagcttc gggcgttttt cgcgcctacc
      181 agcctgaagc cggtcagacg gtgattgatg agtacgtccg cgcacgggta gatcaggtcg
      241 ggttggacac atggcggctg gacctgacga acacggggaa tcagcccatc gaggcggtct
      301 ggtttccgtg ggaatcgtcc ctccaagcgg tggggcagtc cctgggcgac gacgtgacgt
      361 attacccaaa gcagcttggc gtagcggcga aaacggatac tctgccgcct gacgagtgga
      421 tcggccagcc ttacccggga acatgtgggc cacttattgt tcaggcggac cggtgggcgg
      481 cgcggatgat cgccgctgtg aattggccgc cgcggatctt gagtccgctg atggcacgcg
      541 ggcgcatggc gctccgttac gatcagcctc tagcgccgca ggagcagcga tgctacacag
      601 ctctgtgttg cgaggcgcgc ggcgacgagg cgcgcggcga gattccctgg cacaaggttc
      661 tggaccgcta caaggagtgg ctgcttgaga acatgcgccg cgaggggctc taccctattg
      721 cctatcccga accgatgtgg cgcgctaacg ggtgggtgaa cgtccaactt cagaacatac
      781 cccgcttcga cgtgcatgag ttatgggagg cctaccagaa agcccgcgac gtgctgccct
      841 gggtccaatt ctggggacag atgagcaact atcaccggtg tccggccgaa gggaaaaaag
      901 actggccgga tccgccgttg gagccgggtg agaaaacagg ctgctgtctg cccctagcgc
      961 gattccaccg ccgatatatg ccagatgtgg cccacttcgc aagggaagtg tgcgccagtg
     1021 gcggtatagt cggctattac ctccggccct ggccgccaca agaatatctg gccgagggca
     1081 aggacagcct ggcatttctc ctcaactgga ttgaagtgag ccgcgaaggt ggggcgtgcg
     1141 cgttctatct cgacacgctc ggcacgcgcc cgttcggcga cccgttgttg gtagcgcgcc
     1201 tgtttggcga caggttgccg gcgatgtcgg tgattgagtg gccggtggac atctatccga
     1261 cggccttctt ggccagcggt gccctatggg gcggcttaga ttggtcgacg gagcccgggc
     1321 ggacaccggc ggatttgagc gtcacgctac agcgggtgac gtttccagct ttcgggcgtt
     1381 atctcctgga tgatcgggtt ttctttcttg gcgcatcgaa tggcgactac atgtggtggg
     1441 cgaactatcg cggacatcag cactggaccg agcgacaggc gttcttgctg ggaatgaagt
     1501 tcgatgctcc tgcgcatttg ccgaagcacg ccgcaggctg gatcaacccg gcgaatgaag
     1561 tggtgcgggc ggtcgccgat caatggaata aggtgggctg gtgggagcgt cagccgcgct
     1621 atcgcgatcg cgacggcatt tacgaggttt caccgggaat tgacgttcgc cgctttgagg
     1681 atcgggacgg tgtgaccatg ttcgtgctcg ataactggat gcggcgcggc gaagcggctt
     1741 tcacgtaccg aggccgccgg ataattgggc caccgcagca gttatgcgtg atgattctac
     1801 cgcgggaact ggagccgcaa cacgcgcagt gagcgcgagg tctagcacat gtatctagtg
     1861 cttcacatcc tgattacgct tgtgtgcggc acgatatgcc tgctggcggc acggcgcctg
     1921 caggccaagc tgcccaaaat gatcatgttt tcggccctgg ggctcatcca cggggtcgtc
     1981 ccggcgctga cgccgcgcat gttcatgtgg ccgggcgccg cacaagccaa tattgattac
     2041 gccgcgctgt tggcgctgct ggggattctg gcgttcgccc tgggctggcg cattatggag
     2101 cggtctgcag acccccaggg ccttgcaccc ggcttatact attaccttta cagttccgag
     2161 gggcaggcag cgctcaagcg ccttttctgg gcgaccgccg tgctgggctg cgctgcctgg
     2221 ctgggctgca tgattgtcag cgccgggtcg gtttccgcgg ccttttccgc cgcgcgtttt
     2281 cagtaccgca tgcgcggcaa catctatctc acgacattgc tgacgcacgt gtcgagcttg
     2341 atggccgtgc cctgctttac ttgctttttc ctgccccgca ggtaccgcat gcttggggcg
     2401 agcttcggaa tcagcatggc tctgttgctt tttgttgcca gccaaggcgc acgggcagcg
     2461 gcgctgggca tgattggcgc aactggcttg ggatattcgt tcgcacatcg gctcagactg
     2521 cggcgtgccc tgctcgtggg ggctgtcggg ggagcgatac ttctgcttgc agttgcgacg
     2581 tatgaggtgc gaaaagtaat gacccggcgc ccagcatctg aagtcctgag gatgctggtg
     2641 tcgcctgaga cctatcaggg cgcgctgacg cgtgatccct tgtactacca ccagttcctc
     2701 gtcgcagcgg tgatgcactt tccagacctg catccctacc tgaacggggc ggcgtaccag
     2761 cgcatcttat tcttttacct tccacatcgc tatttccccg agttgaagcc tcccgacacg
     2821 cacaacacgt ttgcgcgggt aattacgggc agttcgacgc gcaccacgat accgcccaca
     2881 atgatcggag acggctacat caatttctgg ggactgccag gcgttatagg tatcatgcta
     2941 ttccatggca tgctattcgg gtgggcagag cggaagattc gagagcggct gttggtgttt
     3001 ctggcaatcg gggcgtcatt cgccaattgc gctttgctcg ccgttcgggg ttctccgtat
     3061 gaggtgctga cgctttgcct tagtgcgtcg ggcatattgt gggtcttggg atcggcctgc
     3121 gggctgcgat tccgcaaggc gcgccggagt ccgccagctt tgccctcagt aacgttggtg
     3181 tcgacggctc taagcagcca gccacagata tgagccggct ggcagaacat ggcgcgaagc
     3241 tgagggcgtc tctgcgccgc ctgccaaaca cccgcgtgcg cggcgaaacc acctgggtga
     3301 tcgcccacaa agcggccgaa ttctgcgtca tgctggcggg gctgaagctg ttcacgaacc
     3361 tgatgtcgca ggcctctttc ggcgaatacc agttgggcct gacgacactg gccctgatca
     3421 cccatctctt cgaaacgccc atcagtcagg tctacctgcg gcagtaccac acggctcttt
     3481 cccaaggtac ggcgcgctcc gcactctccc aaaccatgcg ctggctcggt gccacgacta
     3541 tcgtagtggc cggcggatgc gcgctgctca ccaagccgct ggccgatgcg tttgaactgg
     3601 aaactctcac ggtcctcgcc gtaggcctga tcttcctcac caatcgctgg cgatccctgc
     3661 gtgtgcaagt gctggacgtt caacgcaagc gccgccagtg catgttcgag aacctggggt
     3721 tcttcgcctt gcagactacg ctcggcgccg ccgccttgta cttcatccgg ccctcggcca
     3781 cgacagcgct gctgagttac gcactggcag ccggcgtttt cgggagcgtg gccctgcgcg
     3841 gcattcggcg ggatctgaga acgcttccgg ccgggaagcc ggctcctttt gcaggcttgg
     3901 tgtacgcgta tggcgtgccg ctaggactgc tgaccacgtt tcagtggcta cagagtttcg
     3961 gggagcgcta tgtcctctgt agtcagttgg actatgcgac ggtggggcgc tacgtggctg
     4021 cctatcaggt ctgcgggttc cccttcatgg ccatgaacgc cgtgctcacc gcgctggtgc
     4081 agcccgtcgc cttccagcgt gcctcggata tcagcgatcc gcggcaggtg tggtcggctg
     4141 accggctgct gctggccggc atcggactgt atgtggcggc cggggtgttg atcgtcgcgg
     4201 gctacgcgtt ctttgggcca ctgctgatgc aagttctggc ctccgatgag tacgtcctgc
     4261 cggccagcag cttcaccata ttggcggcgg cgcgcatgct gatgttcgcg agcctgcttc
     4321 agcacgtgtt cttcaaagtg catcagcaga cgcgctttct gctgttctac agcgtgatcg
     4381 gtggggtcat actgctgccg gccgcgtggc ttctggtgag cgcgtacggt atgatgggcg
     4441 cggcggtggc ggtgctgacg acggcggtga tctacacgct gttgctggct tttgcgcccg
     4501 gggggatctg gtggctgctt cgtggagttc gcacgcgggc cgccgccgtg cccagcgttt
     4561 cgaccccagg ggccgcaacc gagggctgtt tatgagctac tccgtgctga tgatgcccta
     4621 ctccgtcggg ctcggcatga cctatcacat gggccaaacc acggccgcgc tgctgcgccg
     4681 cgcgacgccg ctgggggtg
//
LOCUS       DAOSYS010000269         4694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15078_length_4694_cov_5.170942, whole genome shotgun sequence.
ACCESSION   DAOSYS010000269 DAOSYS010000000
VERSION     DAOSYS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15078_length_4694_cov_5.170942"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..362)
                     /locus_tag="PLU99_13570"
     CDS             complement(57..362)
                     /locus_tag="PLU99_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18118.1"
                     /translation="MARYRMDDGTVVDTDRASARWEEARRWDGRNQVSVHTGDQWKHQ
                     TLYRSRRGRYYVEHWSQWQGSTAHVEWVSPQEAARWLILNAEELPDDLKQYEADVTE"
     gene            complement(482..1123)
                     /locus_tag="PLU99_13575"
     CDS             complement(482..1123)
                     /locus_tag="PLU99_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO18119.1"
                     /translation="MTLYEKYRPRQFSDVLGNPKAVRCLESACRRGIGGRAVWISGPS
                     GTGKTTLARILAHSVADPFCVTEFDSADDFNAAAIENMRQSMHLYGLGRGGRAWIINE
                     AHGLRKPAIRALLGILERLPSHCVVIFTTTRDGQEIFEDEIDAGPLLSRCLCISLSNQ
                     GLAQAFATRAKQIAQAEGLDGQPIAAYVRLVQRCKNNMRAVLQEIERGAMVEP"
     gene            complement(1277..1555)
                     /locus_tag="PLU99_13580"
     CDS             complement(1277..1555)
                     /locus_tag="PLU99_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18120.1"
                     /translation="MRATMINGNEIQLDAEPIRKQMERYERILTDLDRWARTAQLAAK
                     EARSLDDKLRMTRLQKAINDCRRTLRLQYYEAEDAAALAWQRAESREQ"
     gene            complement(1591..1956)
                     /locus_tag="PLU99_13585"
     CDS             complement(1591..1956)
                     /locus_tag="PLU99_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18121.1"
                     /translation="MPAIYRADVWCDSCADAIKAAIKREGAVPKDTTDERRYDSDEYP
                     KYMSNDEASDSPCHCAAGERCLEAAILPSGRKIGALLSTALTSDGVEYVRDAVREGGE
                     VAEFWREAFSAYDLFNRGA"
     gene            complement(2231..2455)
                     /locus_tag="PLU99_13590"
     CDS             complement(2231..2455)
                     /locus_tag="PLU99_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18122.1"
                     /translation="MQVQINNTVQVVTGKAARIIRAQPGYIASRPKMLSRTKAGGVRL
                     YVAAAGQWMDASLADIHDEAVASLVPESEE"
     gene            3222..3821
                     /locus_tag="PLU99_13595"
     CDS             3222..3821
                     /locus_tag="PLU99_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18123.1"
                     /translation="MTATVTIPAEGEDEKQSRLKRFAEQGPAHLDAYWAAHPEKRRGS
                     DGGRPVGYREKKSTKLKQRRSKVDRSYESLAKAVRGRVASKQVELDWVQQNMSLPLSA
                     LDVAQIPSAGAVAMLKWVKEEGGAKDFFMGLFKSQLPTRKELDAQAALADDGRECVRL
                     IEKLQRLRAEKRKEVAVESQQRGDRHDDGGFDAEGPALA"
     gene            3751..4209
                     /locus_tag="PLU99_13600"
     CDS             3751..4209
                     /locus_tag="PLU99_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18124.1"
                     /translation="MSRNNVVIDMTMGVSTQRVRRWREYRDWAETLRWLISEIGLDDL
                     FADAKATVAPKGAELMGPSVRISLPPGGGNGLVLETRAVYGTDKRLVSERHEVPYVKL
                     QGWDGAPCKEVLRLVGSMLLDWYRHLLVQDWRVAATEPVKVEPVKKEKKA"
     gene            4206..4481
                     /locus_tag="PLU99_13605"
     CDS             4206..4481
                     /locus_tag="PLU99_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18125.1"
                     /translation="MKVQPLQMNPAVDLGAHLANERARQNQELAQRVTMVTKNAQWML
                     GLLVQHTAQVPDVSLLQALTDQAFAWSRYFQQQFEQEADNAGQPAHD"
     gene            4456..>4694
                     /locus_tag="PLU99_13610"
     CDS             4456..>4694
                     /locus_tag="PLU99_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18126.1"
                     /translation="MLANQPTIELGGRRPSLYDDRGDLLTDEQRVWAEGRLYNLAVDV
                     THDKAGLARVNVSGVVPVACGLTCGFAANEDDLQAA"
BASE COUNT          883 a         1324 c         1484 g         1003 t
ORIGIN      
        1 atacgctcgg cccatacacg cgcccccggt tgcccagggg cgggagtgcg ggtcggtcac
       61 tccgtgacgt ctgcctcgta ctgtttgagg tcgtcgggca gctcctcggc gttgagtatg
      121 agccagcgcg cggcctcctg cggcgacacc cactccacat gcgcggtcga gccctgccac
      181 tgtgaccagt gctcgacgta gtaccggcca cggcgcgagc gatatagtgt ttgatgtttc
      241 cattggtcgc cggtgtggac gctcacctga ttgcggccat cccaccgccg ggcctcctcc
      301 cacctggcgc tggcccggtc cgtgtctacc accgtaccat cgtccatccg atagcgtgcc
      361 atctgtgcat ctcctgcggc taagccgcat tcgggtaagc gaaaattcct aagccgtccg
      421 tccggcccag gccctcgcgc cccggcggct cccacggccg gggcgcggtg gcgcgggtcg
      481 attacggctc caccatcgcg ccgcgctcga tttcctggag aacagcgcgc atattgtttt
      541 tacaccgttg cacgagtcgc acgtacgccg caatcggctg tccgtccaag ccttccgcct
      601 gcgctatctg cttggcgcgg gtcgcgaagg cctgcgcaag gccctgatta gataggctga
      661 tacaaaggca acggctcaga agcgggccgg cgtcgatttc gtcctcaaat atctcttgtc
      721 cgtcgcgcgt tgtggtaaaa atcacaacac aatgcgacgg caatcgctcg agaatgccca
      781 acaacgcgcg aatcgccggc ttccgcaacc catgtgcttc gttgatgatc cacgcgcgcc
      841 cgccccggcc caaaccgtaa agatgcattg attggcgcat attttcaatc gcagccgcgt
      901 tgaaatcatc ggcgctgtcg aactcggtga cgcaaaacgg atcggccacg ctatgcgcca
      961 gtatccgcgc cagggtcgtc ttgcccgttc cggacgggcc gctaatccag actgcccggc
     1021 cgccgatgcc gcgcctacaa gcgctttcaa ggcaacgcac ggctttggga ttgcccagta
     1081 cgtccgaaaa ctggcgcggg cgatactttt cgtagagcgt catattgact tttgcttggg
     1141 tttgcagcat gatttggtat ccttttccag tcgccttgcg tcgcgcccgg gcaagggtac
     1201 gcggcgcggg gcgtgttttt gttactatcg gccagcacca gcgcgcccga ttgctcaggc
     1261 gcgctcgtgc gggccgctat tgttcccggc tttccgcgcg ctgccatgct agcgctgccg
     1321 cgtcctctgc ttcgtagtac tgcaggcgca gcgtgcggcg gcagtcgttg attgccttct
     1381 ggaggcgcgt catacgcaat ttgtcgtcga gtgaccgcgc ttcttttgcc gcaagctgcg
     1441 ctgtcctagc ccaccgatct aggtctgtca aaatacgctc atatcgttcc atttgcttac
     1501 gaatcggttc cgcgtctagt tggatttcgt tgccgtttat catcgtagca cgcatggtga
     1561 ctcctatctc gcgcgccact gcggcccgcg ctaagcaccc cggttaaata ggtcataagc
     1621 gctaaatgcc tcgcgccaga attcagctac ttcaccgccc tcgcgcacgg cgtcccggac
     1681 gtattccacg ccatcgctag taagtgcagt tgacagaagc gcgccgattt tacgcccgga
     1741 cggcaaaatc gctgcctcta agcatctttc gccagccgca caatgacacg ggctgtctga
     1801 tgcttcgtcg ttggacatat acttcggata ttcgtcgcta tcatagcggc gttcatcagt
     1861 tgtgtcttta ggtacggcgc cctcgcgttt aattgccgcc ttgattgcgt ccgcgcacga
     1921 gtcgcaccat acatccgctc ggtaaatcgc tggcataatt gtttaccttt attctcacgt
     1981 ccggcccatt ccggctcgtg ttcgctggcc gtcacgcacg gcccaagggg cgccgtccgg
     2041 ttgaccaggc ggcgccgcgc gggtcgtcag gccgtcagcg ccgcaattgc cttatctgcc
     2101 gcgcgataac gggaaatgaa cgaggcccaa aacacgcggc cgttatgctt aattccaatt
     2161 cgcatgattt tgctcctgag taagcggcgc gccgggatgc cggagcagag cccggcgcgc
     2221 gggtttagga ctattcctca gactcgggaa cgagggacgc aaccgcctca tcatggatgt
     2281 cggcaagcga tgcgtccatc cactggcccg ccgctgcgac atacaggcgg acgccgccag
     2341 ccttggtacg cgacagcatc ttgggcctgc tggcaatgta gccgggctgg gcgcggatga
     2401 tccgcgcggc cttgccggtc acaacctgga cggtgttgtt gatctggacc tgcatgacat
     2461 gctccttgtg atttgctcct aaaccgtttt cgctccggcc tgatttcgcg cggtaggccg
     2521 gaaaccgcta tgccttaaac ataccacacg atcggctggt tgtcaaaaaa aaatctttac
     2581 gtttgcgaaa aattttccga tgggctattc cggaacaaac gaagtcttgt ttcgattcgc
     2641 cacaatttca tcaaaaaaag ccttgtttta tcgctgttat aattccgact tgacaacagc
     2701 tagatttgtc gttataggct aggcgcgcaa gtatacctac accttagatt caccttgaaa
     2761 acaagcttaa aacacgagcg agaccagcaa gcgcacgaga ccagtagcac gagcgaaggg
     2821 aagacaaggc acaagccgtg acgatgatgg tagggttgag ggtgagggtg agggtgaggg
     2881 tggcgtgtaa atcatatgcc tttggtccta tatggttcta cagcgttctg cgacgcgcgc
     2941 tttgcatgca gtttcacaaa gccttgtatg cggtttccag agcttcaaaa cactttccac
     3001 ttcataccaa gtgtaaagat gtatcagctt atgggaccct aaacacactg gcggaaacgt
     3061 aaccacgacc ccggctggcg cgcgtcgcag agcgttagtt aattccctcc tcccccacac
     3121 ccgctttgac tctacttgct tgcattccta ggctttcggc atatacttag tcttgtagga
     3181 tttcacgtgg aacgctacta cgggttgtgg agcggctggg gatgactgcg actgtgacga
     3241 ttccggctga aggggaggat gaaaagcagt cacggcttaa gcgttttgct gagcaaggtc
     3301 ctgcgcatct agatgcttac tgggccgctc atcctgagaa gcgtcggggc tcggatggtg
     3361 gtcggccggt ggggtatcgc gagaagaagt ctacgaagtt gaagcagcgc cggagcaagg
     3421 ttgatcgcag ttacgagtcg ttggccaagg ctgtgcgggg gcgggtggcg agcaagcagg
     3481 ttgagctgga ttgggtgcag cagaacatga gtttgccgct gtcggcgctg gacgtggcgc
     3541 agataccgag tgcgggggcg gtggcgatgt tgaagtgggt gaaggaggag ggtggggcta
     3601 aggacttctt catggggctg ttcaagagcc agttgccgac gcgcaaggag ttggatgctc
     3661 aggcggcgct ggcggatgac gggcgggagt gtgtgcggtt gatcgagaag ttgcagcggt
     3721 tacgggcgga gaagcgaaag gaagtggctg ttgagtcgca acaacgtggt gatcgacatg
     3781 acgatggggg tttcgacgca gagggtccgg cgttggcgtg agtaccggga ctgggccgag
     3841 accctgcgct ggctgattag cgagatcggg ctggatgacc tgtttgccga cgccaaggcg
     3901 acagtcgcgc ccaagggtgc cgagttgatg ggacccagtg tgcgcatcag cctgccgccg
     3961 ggtgggggca acgggctggt gctggagacg cgggcggtct atgggaccga taagcgtctg
     4021 gtgtcggagc ggcacgaggt cccgtatgtg aagcttcagg gctgggatgg ggcgccgtgc
     4081 aaggaggtgc ttcggctggt ggggtcgatg ctgctggact ggtatcggca tctgctggtg
     4141 caggactggc gggtggcggc aactgagccg gtcaaggttg agccggtcaa gaaggagaag
     4201 aaggcgtgaa agtccaaccc ctacagatga acccggccgt tgacctgggc gcgcacttgg
     4261 cgaacgagcg ggccaggcag aatcaggaac tcgcgcagcg tgtgacgatg gtgacgaaga
     4321 acgcgcaatg gatgctgggg ctgttggtgc agcacactgc acaggtgcca gatgtgtcgc
     4381 ttttgcaggc actgacggat caggcgttcg cgtggtcgcg ttacttccag cagcagtttg
     4441 agcaggaggc cgacaatgct ggccaaccag cccacgattg agcttggcgg gcgccggccg
     4501 tctctctatg acgaccgcgg cgacctgttg acggatgagc agcgcgtgtg ggcggagggg
     4561 cgactgtaca atctggcggt cgatgtcacg cacgacaagg cgggcttggc acgggtgaac
     4621 gttagtggcg ttgtgccggt cgcgtgtggg ctgacgtgcg ggttcgcggc aaacgaggac
     4681 gatcttcagg cggc
//
LOCUS       DAOSYS010000270         4678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15141_length_4678_cov_5.025741, whole genome shotgun sequence.
ACCESSION   DAOSYS010000270 DAOSYS010000000
VERSION     DAOSYS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15141_length_4678_cov_5.025741"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2494)
                     /locus_tag="PLU99_13615"
     CDS             complement(<1..2494)
                     /locus_tag="PLU99_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO18127.1"
                     /translation="MIEALIAKVESSTGLASDVKAYDLKHARAEAVAQNLDQMLKAKV
                     AERDAGRQGAVRTAVFSDINTNRLFVLAPQEYQDLAARLVEIVDTEAATTDVVHIITL
                     EQGDAQQIAQTLSQLIAGNRQGSGTAAGKSGTATVRITADPGSNSILLAGMPKDLGEV
                     EQWIRELEVNSSTVPELQIIPLKNSNAVTVARMVSDIFAASGRGRGSAIDAVTITPDE
                     YHNRLLVTTNKRKMRQVEATIAMLDAEPEGDQDALTDPSGRKLYFVDIGRGDASDIAY
                     DVRDMFPDEDQGGPSIKSDWFGEYITVRCRPHEIEPIVAVIRQFEAHAKPEKKVVILP
                     IRGPVKDLMGYLEARHAGELMVEYAKQDVAPTMVETLWEEGEEPPAIRHKRDRQRLNK
                     ETGNDAGATRDSKRTDVRPFQLGMPVTVALLSDIDLALLSGEPAPDPAESESAATPAA
                     SSKRALPPGVRCAGEPEAGADQSGPRKEKPRVVVQPDGRSVIIYGDKSQVEELEDTIT
                     LLEEDLNVGEVVRIFEFKYGDVSAAAEVIQIMFNERPQLRLPQQPPQQRGQRGDKDDD
                     HQSMIERLMGGNQRGQRGGRTSVDGQRVRIATDPGHNYLIIKCNQADLPEIKQLLREL
                     DIPPGEVDLRIFQLRNLVASETAEDIKAVLGIAKAQQKRGGAAAPARRGAAGGFNPQA
                     QLIEMLQQQAFSVPGVEGGAKVEQVEVVPNDVTNSLLVSAPPEVMKVIEKVIGDLENL
                     EGREIVGIYEYQLEHAKMEDILPLLQDIFAAARAGGGGPSQPRAGGGMAAAVRGRSGG
                     SPGSLGPVTVSGDPRVNKVIFTCEKKDEELVRA"
     assembly_gap    2598..2607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2608..>4678)
                     /locus_tag="PLU99_13620"
     CDS             complement(<2608..>4678)
                     /locus_tag="PLU99_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO18128.1"
                     /translation="ATEVAELLNKILSLEQNQPQQPDWQRLRQLQMQQQQARGRRGRA
                     PAQQAAPVVATGAVSPEDLLGEGLFERVKKTIRIAADERSNSLIVYANKDGHERVLNL
                     LKEIDRALPTNLQTIQVEHAQAAQIYPTVNQIVQNLPAAGAGRRTPSIVLDEAKNQFY
                     VLAERDQLQQIQEVVQKLDVPGPESVRHMVSLTNIAPSRVAQLIEPILTSGSAGSSKS
                     SAPSIPPALRRLRGMRGMQAAPTPAPTPQATQADNVEILALDEAMLLIVVCPEELWAK
                     VEDTIYLWDEGAVANTPRLETIPIQEGDPQSIAATLNNLYRGTYTHPVLGQSQVIMQP
                     EGRDILVYAIQPAIDEITTLIAALDHEDSTKYEIFPLANADAATVAQQAQALFGGGGA
                     TVVTGPRSRFAPVPATGGSSSSLLIQAEPTTNSLIMQGEPVTLAKVKQFALEADQRAA
                     DLSPIQKIFAMQRARAAEVAQAVQNAYGGQRQRLGGTMSPTQVRAFATGAQVVVEAPK
                     EKMEDIAGFIAQLDSIKPNEIQIKAHKLPGVDVIQMAQNLSATARTIQRPDGLTAVFI
                     PDAASETLVVSAPGDMMTQVDEWVATFGTTADIMPVTIEVQNADPNQVAQAIREMYGR
                     GGRNPQQDVQVTVSNGMLVVKTGPDRLKQVRELVAALDAENNQNLQIKTYQLKIMNAM
                     QVAAQVQM"
BASE COUNT          680 a         1464 c         1536 g          988 t
ORIGIN      
        1 gggcgcggac cagttcctcg tctttcttct cacaagtgaa gatcaccttg ttgacgcgcg
       61 ggtcgccgga gacggtaacc gggcccagcg aacctgggga accgccgctc cgcccgcgga
      121 ctgccgctgc cataccgccg ccagcgcgcg gttgcgaggg tccgcccccg ccggcgcggg
      181 ccgcggcgaa gatgtcctgc aatagcggca ggatgtcctc cattttggcg tgctcgagct
      241 gatattcgta aatcccgacg atctcgcgtc cctcgaggtt ctccagatcg ccgatgacct
      301 tctcgatgac cttcatcact tccggcggcg ctgaaaccag cagcgagttg gtcacgtcat
      361 tcggcacgac ctccacctgc tcgactttcg ccccgccctc aacgccggga accgagaacg
      421 cctgctgctg aagcatctcg atcaactggg cttgcggatt gaagccgccc gccgccccgc
      481 gccgcgccgg cgcagccgcc ccgccacgct tctgctgcgc tttggcgatg cccaaaaccg
      541 ccttgatgtc ctcggcggtc tcgctggcca ccagatttct gagctggaaa atgcgcaggt
      601 ccacctcgcc cggcggaatg tccagttccc gcagcaactg cttgatctcg ggcaggtcgg
      661 cctgattgca cttgataatg aggtagttgt ggcccgggtc ggtcgcgatc cgcacccgtt
      721 gtccgtcaac gctggtccgg ccgcccctct ggccgcgctg attgccgccc atcaagcgct
      781 cgatcatgct ctggtgatcg tcatccttgt ccccccgttg cccgcgctgt tgcggcggct
      841 gctgtggaag gcggagctgc ggccgctcgt tgaacatgat ctggattact tcggccgcgg
      901 cgctgacgtc gccgtacttg aactcgaata tgcggacaac ctccccgacg ttcaggtctt
      961 cctcgagcag ggtaatagtg tcttcgagtt cttcgacctg gctcttgtca ccgtagatga
     1021 tgacgctacg accgtccggc tggacgacca cgcgcggctt ttccttccgc gggccggact
     1081 gatccgcgcc ggcttcaggc tcacccgcgc agcgtacgcc cggcggcagc gcccgcttgc
     1141 tcgacgcggc gggcgtcgcg gcggattccg attcagccgg atccggcgcc ggctcaccgc
     1201 ttaagagcgc caagtcgatg tccgacagca gcgccaccgt tacgggcatg ccgagctgga
     1261 aggggcgcac atctgtccgt ttggaatcgc gcgtggcgcc tgcgtcattt cccgtttctt
     1321 tgttaagacg ctggcggtcg cgcttgtggc gaatggccgg cggctcctcg ccttcctccc
     1381 agagggtttc gaccatggtt ggcgcgacgt cctgcttcgc gtattcgacc atcagctcac
     1441 cggcgtgccg cgcctccaga taccccatca ggtccttgac cggccccctg atgggcagga
     1501 tcacgacctt cttctccggc ttggcgtgag cctcgaactg tcgaatgacg gccacgatcg
     1561 gctcgatttc gtgcggccgg catctgaccg ttatgtactc gccaaaccaa tctgacttga
     1621 tggaaggacc gccttggtcc tcgtctggga acatgtcccg cacatcgtag gcgatgtccg
     1681 aggcgtcgcc gcgaccaata tccacgaagt acagcttgcg gccgctgggg tcggtgagcg
     1741 cgtcctggtc gccttccggc tcggcatcga gcatggcgat ggtggcttcg acctggcgca
     1801 tcttgcgctt attggtcgtg accagcaggc ggttgtgata ttcatcgggg gtgatggtga
     1861 cggcgtcgat ggcagagccc cggccgcgtc cggaagcggc aaagatgtcg ctgaccattc
     1921 tggccacagt gacggcgttg gagttcttga gggggatgat ttggagctcg ggtaccgtgg
     1981 agctgttgac ctccagttcc cgaatccact gctccacctc acccaggtcc tttggcatgc
     2041 cggccagcag gatggaattg ctgccgggat ctgccgtaat gcgcacagtg gcggtgccgc
     2101 tcttgccggc tgcggtgcca ctgccttgcc ggttgcctgc gataagctgg ctcagcgtct
     2161 gcgcgatctg ctgcgcatcg ccctgctcca gggtgatgat gtggacgacg tcagtggtcg
     2221 ccgcctcggt gtcgacgatc tcgaccaggc gggccgccag gtcctggtac tcctgcgggg
     2281 ccaggacaaa gagccggttg gtgttgatgt ccgaaaagac ggcggtacga acggcgccct
     2341 ggcggccggc gtcgcgctca gccaccttgg ccttgagcat ttggtccaga ttctgggcca
     2401 cggcttcagc gcgggcatgc ttcaggtcgt aggccttgac gtccgaagcc agaccggtgc
     2461 tggattcgac cttcgcgatc aaggcttcga tcatgggcaa ctgtttttcc ggggcaatca
     2521 ccaccagggt gttggtggcc ggctcggcga acgcgccggg ctgcatgccg gagcgctgcc
     2581 ccggtgacat gctgcgcnnn nnnnnnnaca tctgcacctg cgcggccacc tgcatggcgt
     2641 tcatgatctt cagttggtag gtcttgatct gaagattttg gttgttttcg gcgtccagag
     2701 cggcgaccag ttctctgacc tgtttcaacc gatccgggcc ggtcttgacc accagcatgc
     2761 cattgctcac ggtgacctgg acatcctgct gcggatttct cccgccgcgg ccgtacattt
     2821 cccggatggc ctgggcgacc tggttcgggt ccgcgttctg cacttcgatc gtgaccggca
     2881 tgatgtcggc ggtcgttccg aaggtggcga cccactcatc gacctgggtc atcatgtcgc
     2941 cgggcgcgct gacgaccagg gtctcgctgg cggcgtcggg aatgaagacc gccgtcaggc
     3001 catcggggcg ctggatagtc cgcgcggtgg cggagaggtt ctgggccatc tgaatgacgt
     3061 cgacgcccgg cagcttgtgg gccttgatct ggatttcatt gggcttaatg ctgtcgagct
     3121 gcgcgatgaa gccggcgatg tcctccattt tttccttggg agcttcgaca acgacctggg
     3181 ccccggtggc gaaagcgcgc acctgagtag gcgacatcgt gccgcccaag cgttgccgct
     3241 gtccgccgta ggcgttctgc acggcctggg cgacttcggc ggcccgggcg cgctgcatgg
     3301 cgaagatctt ctggatgggc gagaggtccg cggcccgctg gtccgcctcg agggcaaact
     3361 gcttgacttt ggccagggtc accggttcgc cctgcatgat gagcgaattc gttgttggct
     3421 cagcctggat cagcaggctg ctgctgctgc cgccggtcgc gggcacgggg gcgaatctgc
     3481 ttcggggccc ggttacgacg gtggcgccgc cgccgccgaa caaagcctgg gcctgttgtg
     3541 cgactgttgc ggcgtccgcg ttggccaggg ggaaaatctc gtacttggtg gaatcctcgt
     3601 ggtcgagggc tgcgatcagc gtcgttattt cgtcaattgc gggctggatc gcatagacca
     3661 ggatgtcgcg gccctcgggc tgcatgatga cctgcgattg cccgagcacc gggtgcgtgt
     3721 aggtgccgcg atagaggttg ttgagcgtgg cggcgatgct ctgcgggtcg ccttcctgta
     3781 ttgggatagt ctccaagcgc ggcgtgttgg ccacggcgcc ctcgtcccag aggtagatcg
     3841 tgtcctctac tttggcccac agctcctcag ggcataccac gatcagcaac atggcctcgt
     3901 cgagggcgag gatttcgacg ttgtctgcct gcgtggcctg gggagtcgga gcgggagtgg
     3961 gggcggcctg catcccgcgc atgccgcgca gccggcgcag cgctggcggt atgctcggag
     4021 cgctcgactt actggagccg gctgagccgc tggtcagaat gggctcaata agctgtgcca
     4081 cacggctggg ggcgatattc gtcagactga ccatgtgccg gacgctctcg ggtccgggca
     4141 cgtccagctt ttggaccact tcctgaatct gctgtagttg atcgcgctcg gccagcacgt
     4201 agaactggtt cttggcttca tccagcacga tgctgggcgt ccttcggccg gcgccggccg
     4261 ccggcaggtt ctgcacgatc tgattgaccg tgggatagat ctgcgcggcc tgggcgtgtt
     4321 cgacctggat cgtctggaga ttggtgggca gggcgcgatc aatttccttc agcaagttaa
     4381 gtacgcgctc atgaccgtcc ttgttggcgt agacgatgag tgaattgctg cgctcatcgg
     4441 ccgcgatgcg gatggtcttc ttgacgcgct cgaagagccc ttcgccgagg aggtcttcgg
     4501 gactgacggc gcccgtggcg acgaccggcg ccgcctgctg cgcaggggcg cggccgcgcc
     4561 ggccacgcgc ttgctgctgt tgcatttgca actgtcgcag ccgctgccag tcgggttgct
     4621 gaggctggtt ttgctcgagt gagaggattt tgttgagcaa ctcggcgact tcggtggc
//
LOCUS       DAOSYS010000271         4649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15264_length_4649_cov_5.453200, whole genome shotgun sequence.
ACCESSION   DAOSYS010000271 DAOSYS010000000
VERSION     DAOSYS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15264_length_4649_cov_5.453200"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLU99_13625"
     CDS             complement(<1..699)
                     /locus_tag="PLU99_13625"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOO18129.1"
                     /translation="MLTTSYAGKEIALTLLVGLAVTVVIGWFFGWWAILPAVIALALL
                     LFYRDPVRRIPEGRGLVLAPADGRIVAIERNLPGPDGQGRSLRIMTFLAVYNVHVNRS
                     PCAGRVRGVEYKPGKFLNALRDEADACNECNTVTLTPAAPLPGPVYVRQIAGALARRI
                     VCTLKEGQNVAAGERIGMIKLGSRTELRLPESDDWDVLVRVGQRVAAGKTVLLRLRTA
                     VPAVSEAGRPVEAQV"
     gene            complement(693..1559)
                     /locus_tag="PLU99_13630"
     CDS             complement(693..1559)
                     /locus_tag="PLU99_13630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOO18130.1"
                     /translation="MCTPGHCELLAIDLDGTLLDSSHRVPPENRAALHAAHAAGIRIV
                     LCTGRSFPETRPVLDEIGLDLDATVTVFGAVLTDVRTGRTLERVAFDLDLAHRLTNWF
                     RRRGYAVLWLNDAEEAGVDGYVIDGPRRHPAVDRWVQNTPCRVLCVPDVSDVACPPLR
                     ISIIDDPDVLEELNPEVHREFDGQIAYNLLRAPAYSLTIIEAFAPQANKWYGIQRLCQ
                     RWGLDASRTVAIGDDINDLAMVQHAGLGVAMANAHPAVRQAARLVVGDNDSCGVAELI
                     ERLLDGTRRSEC"
     gene            complement(1911..2402)
                     /locus_tag="PLU99_13635"
     CDS             complement(1911..2402)
                     /locus_tag="PLU99_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOO18131.1"
                     /translation="MIRRLVYSVTVLGLVVSPALADIAVTLEPGGGLTGLIYADIPIQ
                     DAIVAFGLDAPSQNPAMTLTSFTAGPLFAQPASTPDGDGIAGLVLSGAVYGEHVLLGT
                     LTYDGDPAGGWVDLGVTPGDLTEGLALPGFGFATATFSGAYIPEPAALALLAIGAFAL
                     RRR"
     gene            2678..2935
                     /gene="yacG"
                     /locus_tag="PLU99_13640"
     CDS             2678..2935
                     /gene="yacG"
                     /locus_tag="PLU99_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOO18132.1"
                     /translation="MTAGNAVGGRVYSLRMRTYACVICKRTVQFEGKLPPEYPFCSER
                     CRMIDLGLWFREAYSIDRDLTPEDLSDSARLAGDQGEPRSG"
     gene            complement(2932..>4649)
                     /gene="xseA"
                     /locus_tag="PLU99_13645"
     CDS             complement(2932..>4649)
                     /gene="xseA"
                     /locus_tag="PLU99_13645"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOO18133.1"
                     /translation="NAARRRDRSRANPSRITGAPRKADPFHPLRHLATGNTKRETPFV
                     SDRPLFDPARIRLPGQARPGEAAARGGEAGPPTYSVRQVNELVRGALLRHLPPTINVL
                     GEIGDFSRPSSGHLYFTLKEAGSELACVMFRSAAVRVRFEPQAGMEVIASGTLDVYMP
                     RGSYQLYVRKLEPRGVGALELAFRQLKEKLEREGLFDAPRKKPLPAVPQRIAVITSPT
                     GAAVRDILQTLRRRFPALHVLVFPVRVQGEGAALEIAAAIRAMNAHAAALGGIDAAIV
                     GRGGGSLEDLWPFNEEVVARAIAASTIPIISAVGHETDISISDLVADVRAATPTAAAE
                     LIAPRVSDLLDWLDQRMRRVARGAGHAIQLARARLSATLAYDGLARPLRRLRERAQML
                     DELQHRLQVAAYCRFRDARQRLNRAELALLRFRSGGALRRLDERVSDRLHRVLRGLIQ
                     PVRVRERLLANRLARLQRVSPERRLAAANEHLQQEMQRLHLGMRGILHARRERLERRL
                     EALTAYSPQNVLNRGYSITRDARTHEIIRSIDQIREGVRLITRVSDGEFRSRAEDPRQ
                     GRLFD"
BASE COUNT          810 a         1598 c         1494 g          747 t
ORIGIN      
        1 cacctgcgcc tctaccggtc gccccgcctc cgaaactgcc ggaacggccg ttcgcagccg
       61 cagcaagacc gttttgcccg ccgctacgcg ttgtccgacc cgcaccagca cgtcccaatc
      121 gtcgctttcc ggcaggcgta gctctgttcg tgagccgagc ttgatcatgc cgatccgctc
      181 gccggccgcc acgttctggc cctctttgag cgtacagacg atccggcggg ccagggcgcc
      241 cgcaatttgc cgcacgtaca ccgggccggg caacggggca gccggtgtaa gcgtgaccgt
      301 gttgcactca ttacacgcgt cggcttcatc gcgcagcgcg ttaaggaatt tgcctggctt
      361 gtactccacg ccgcggacgc gaccggcgca tggactgcga ttcacgtgga cgttgtaaac
      421 cgcgagaaat gtcattatcc gtagcgagcg tccctggcca tccggtcctg gcaggttgcg
      481 ctctatggcc acaatgcgtc catcggccgg cgccagcacc aatccccggc cctcaggtat
      541 gcggcgcacc ggatcgcggt aaaacaacaa aagggccagc gcgatcacgg ccggcaggat
      601 cgcccaccag ccgaagaacc agccaattac taccgttact gccaagccga ccaacagcgt
      661 cagcgcgatt tccttgccgg cgtaagacgt ggtcagcatt cagatctccg ggtcccgtcg
      721 agcagccgct ctatcagctc cgccacgccg cacgagtcgt tgtccccgac taccagccgg
      781 gcggcctgtc gaacggcggg atgggcgttt gccatcgcca cgcccagccc cgcgtgttgc
      841 accatcgcca gatcattgat gtcatcgccg atcgcgaccg tgcggctggc gtccagcccc
      901 cagcgctggc acagccgctg gattccatac cacttgttcg cctgcggcgc gaacgcttcg
      961 atgatagtca gcgaataggc cggcgcacgc aacaggttgt acgcgatctg cccgtcgaat
     1021 tcgcggtgca cctccgggtt cagttcttcg agcacgtccg ggtcgtctat gatcgagatt
     1081 ctcagcggcg ggcaggcgac gtcgctgacg tccggcacgc acagcacgcg gcagggggtg
     1141 ttttgaaccc agcgatcaac ggccgggtgt cgccgcgggc cgtcaatgac gtagccgtcg
     1201 acgccggctt cctcggcgtc gttcaaccag agcacggcgt agccgcggcg gcggaaccag
     1261 ttagtcaggc ggtgcgccag gtccagatcg aatgccacgc gctccagcgt tcggccggtg
     1321 cggacgtcgg tcaataccgc gccgaagacg gtcacggtgg cgtccaggtc gaggccgatc
     1381 tcgtcaagga cagggcgcgt ttcggggaag ctgcgcccgg tgcagaggac gatgcggatt
     1441 ccggcggcgt gcgcggcgtg caaggcggcg cggttttccg gcggcacgcg gtgcgatgaa
     1501 tccagcagcg tgccgtccag atcgatggcc aacagttcac agtgtccagg ggtgcacacg
     1561 gcatcctcag cgcttgtcaa agccgcgatt gtcgcatggc ggaggcgggg tgaaaaggtc
     1621 cgcgcgttat tgagagggat cgagggatca agaagagcag agccggggcg tgtcgcaaag
     1681 cagagccggg gcgccgcccc ggaatccgcc aagaatctcc cacccgcgta gtccggcggg
     1741 cgcggcgggc gacgaatttc gcggaattcc cctgaggtgc gaaagtcggg taagcatgcg
     1801 cggaagccgc ttgcgggtcc gggggacctt cagcggcttc cgcttccgac ttctgagagt
     1861 cgcctgtcgg caacaacctg gtgagccggc acgccggccc gaaccgtctc ttagcgacgg
     1921 cgcaacgcga acgcgccgat ggccaacagc gccagcgcgg cgggctccgg aatgtacgcg
     1981 ccgctgaagg ttgcggtcgc gaacccaaag cccggcaggg cgagaccttc agtcaggtca
     2041 ccaggggtga cgcccagatc gacccagccc cctgcaggat cgccgtcata cgtcagcgtg
     2101 ccaagcagca catgctcgcc gtagaccgct cccgacagca ccagaccagc gatgccatcg
     2161 ccgtccggag ttgacgcggg ttgggcgaat agcggcccgg ctgtgaagct ggtcaatgtc
     2221 atcgctgggt tttggctggg cgcgtcgagg ccgaaagcga cgatcgcatc ctgaatcgga
     2281 atgtcggcat agatcaagcc ggtcaatccg cccccaggct ccagcgtcac ggcgatgtca
     2341 gccaaagccg gactgaccac caaccccaag acggtgacgg agtaaacgag tcttcggatc
     2401 attcctctac cctcctgtcg gtactgagtt tcttgaccgc tcgattctag cccaggcgaa
     2461 tccaagaaag tcaaactttg ggctgcgccg cgttttaggc ggccgcgctg gggagaaagg
     2521 tgctctggtg gttgggaaac gccaacagta tcggtcccgc caaaacgcgc tacgaagcag
     2581 gcccgattaa cgttggcgaa gcaggggaag cacccaataa tgtcttcgcg ctataggacc
     2641 ggacgacccg tgccggcgtg gtagaatctc ggcctccgtg accgcaggca acgccgtcgg
     2701 cggccgcgta tactccttac gtatgcgaac ctatgcctgc gtaatctgca agcgaacggt
     2761 gcagttcgaa ggaaaactgc cgccggagta ccctttctgc agtgagcgct gccgcatgat
     2821 cgacctcggc ctatggttcc gcgaggctta ctcgatcgac cgcgacctga cgcccgagga
     2881 cctctccgac agcgcccggc tcgcggggga ccaaggcgag ccgcgctcgg gctaatcgaa
     2941 cagccgccct tgccgcgggt cttccgcccg gctgcgaaac tcgccgtctg acacgcgggt
     3001 aatcaatcgc accccttcgc gaatctggtc gatgctgcgg atgatttcgt gcgtcctcgc
     3061 gtcgcgcgta atgctgtaac cgcggttcaa tacgttctgc gggctgtacg cggtcagcgc
     3121 ctccagccgc ctctcgagtc gctcgcgccg cgcgtgcaga atgccgcgca tccccaggtg
     3181 cagccgctgc atttcctgct gcagatgctc gttggcggcc gcgagccggc gctccggcga
     3241 aacgcgttgc aggcgggcca ggcgattcgc cagcagccgc tcgcgcaccc gcaccggctg
     3301 gatcaggcca cgcagcacgc ggtgcaaccg gtccgagaca cgctcgtcca gccgtcgcag
     3361 cgccccgccc gaccgaaacc gcaacagcgc cagctcggcc cggttcagcc gctggcgggc
     3421 atcgcggaac cggcaatacg ccgccacctg caaacgatgc tgcagctcat ccagcatctg
     3481 tgcccgctcg cgcagccgcc gcaacgggcg cgcgaggccg tcgtacgcca gcgtcgcgct
     3541 tagccgggcc cgcgccagtt gaatagcgtg tcccgcgccg cgcgccacgc gccgcatacg
     3601 ctgatcgagc cagtccagca ggtctgacac gcgcggcgcg atcagttccg cggcggccgt
     3661 cggcgtcgcc gcacgcacgt ccgcgaccag gtcgctgatg ctgatgtctg tctcgtgtcc
     3721 gaccgcgctg atgatgggaa tcgtactggc cgcgatggcc cgcgccacga cttcttcgtt
     3781 gaacggccac aagtcctcga gcgagccgcc gccgcgcccg acgatggccg cgtcgatccc
     3841 gcccagggcc gcggcgtgcg cgttcatcgc gcgaatcgca gccgcgatct ccaacgcggc
     3901 cccctcgccc tgcacgcgaa ccgggaacac cagaacgtgc aacgccggaa accgccgccg
     3961 cagcgtctgc aaaatgtccc gaacggccgc cccggtcggg ctggtgatga ccgcgatccg
     4021 ctgcggcacg gccggcaggg gcttcttgcg tggggcatcg aacaggcctt cgcgctccag
     4081 cttctccttg agctgccgaa acgccagttc cagcgcaccc acgccgcgcg gctccagctt
     4141 gcgcacgtac agttgatagc tgccgcgcgg catgtacacg tccagcgtgc cggaggcgat
     4201 gacttccata cccgcctgcg gctcgaaacg cacccgaacg gccgcgctgc gaaacatcac
     4261 gcaggccaac tcgctgccgg cctctttcag tgtgaaatag aggtgtccgc tggaaggccg
     4321 cgagaagtcg ccgatttccc cgagcacgtt gatggtgggc ggcaggtgcc gcagcagcgc
     4381 cccgcgcacc agttcgttca cctggcgcac gctgtacgtc ggcggcccag cctcaccccc
     4441 gcgcgccgcc gcctcccccg ggcgcgcctg cccaggcagc cgaatgcgag ccggatcgaa
     4501 tagtgggcgg tcagacacga aaggagtttc acgcttcgtg tttccggttg caagatggcg
     4561 gagcggatga aacgggtctg ctttgcgcgg tgcgccggtg atgcgagacg gattcgcccg
     4621 ggagcgatcg cggcggcgtg cggcgttac
//
LOCUS       DAOSYS010000272         4645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15281_length_4645_cov_6.143355, whole genome shotgun sequence.
ACCESSION   DAOSYS010000272 DAOSYS010000000
VERSION     DAOSYS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15281_length_4645_cov_6.143355"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1250
                     /locus_tag="PLU99_13650"
     CDS             111..1250
                     /locus_tag="PLU99_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOO18134.1"
                     /translation="MNSKQRVLATLAGELPDRVPIGEFAVDFDTVERLLGHETYLRAK
                     AKSQIAFWEGRHAEVAESYIKDHIALHETLELDIVTFPAATWRIPPETDDAPPRRTSP
                     DTWEDKYGRVYRYSAASEDITCIHDPVADAETLSVADFEGPLRPPAIDARSWHILDKV
                     IQRFKDEKFICGPSGGEIGIVLLGGLTRGCRRLARQPEVVRAATRRMLEEQNLADEQL
                     VHADCDGVLWDQDFAYNAGPFISPAVFRELFLPANRQRVARLHEVFGKKVLKHCCGNI
                     RALQDCFLEIGYDAYQSIQASAGMDLATLKREIGGKMTLWGGVAVEHLISGAPEDVRD
                     DVRRAMACGKPGGRFILGASHSIAVGTRYDNYMAMLDEYHRLCEY"
     gene            1307..3781
                     /locus_tag="PLU99_13655"
     CDS             1307..3781
                     /locus_tag="PLU99_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOO18135.1"
                     /translation="MHLLCNSHVDAAWLWEWQEGAAEALALARTVADICEKFPGLVFN
                     RNEAMFHEWIAEYDPALFQRIRKLVKSGAWHIMGGWYLQPDCNIPGGESFVRQILVGK
                     RYFKRVFGVDVKTAANLDSFGHSRGLVQILARSGYDSYLFCRPPQRDADLPATQFVWV
                     GYDGSQVLATLADSHYNSAPGGARQKVVDWIGANGGQACGIVPWGVGNHGGGPSRKDV
                     SELNALMREMPDVDIRHSTPEQYFADLRRRRSELPQHGGDLNPWAVGAYTSMARVKQK
                     HRKLENELYATEKMAATAAIQGLMRYPRTEFYEATRDLLASEFHDSLAGTVIEPVEDA
                     VIQRLDHGLEVVSRIKMRAFLTLAAGQPAARPDEFPVLVYNPHPYPVRTLVECEMQTP
                     WPHRTGRFGVPQVRQGRRLLPAQAEKEHANINEDHRKRVVFAAELAPSQMNRFDCRVV
                     LQPKRPAFGLKESRGVIRLKTASMEVVINTRTGLLDRYRVGGCDYLARGAFRPLVMQD
                     DADPWGMRVRRFRTPAGRFRLMSRVRGARFSAVGDEHLPCVRVIEDGPVRSVVEVLLE
                     YEDSCICQRYKLPKAGTEIEVEARVHWYEKDRMLKLSIPTLVPSPAFAGQTAFGVAEL
                     RADGDEQVMQKWLAVTSKDSDMALTIINDRIHGADCADGEVRLSLLRSPAHAGHPLGN
                     GRPITKQDRFTPRIDQGEHVFRFWLNAGAAQERLAHVDREALWHNEQPYALCVSPGVP
                     ERAAPRPGVVLSDDVIQMSAMKLAEDNNDLIIRLFEPTGRKRTTRLSLPGAGVQAKLA
                     FVPFEIKSLRVNSRTGAVREVDLLEK"
     gene            3795..>4645
                     /locus_tag="PLU99_13660"
     CDS             3795..>4645
                     /locus_tag="PLU99_13660"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO18136.1"
                     /translation="MPGPVSIVLSGLGGYGLCYLEGLLDQPDDGRFRLVAGVDPAPQN
                     CKRLADLEARRVPVYPSLEEFYRVGRADLAVLSSPIHQHCRQTCLALAHGSHVLCEKP
                     AAATVQDVDRMIRARDEARKSVAIGYQWSFSTPIQRLKRDILAGEFGAPRRLRSVCLW
                     PRDEVYYTRNDWAGRQRAADGSWILDSPVNNAMAHDLHNMLYLLGPSIDRSAQPVEIV
                     AELYRANDIENFDTAALRVRTGSGAEVLFYGSHAIAEDWGPEFCFEFERATVTYVTNR
                     EPIVARFR"
BASE COUNT          866 a         1301 c         1604 g          874 t
ORIGIN      
        1 ggcgaaacga ccggcgtttt tgtgggtgac agagtctatt cgtatgtgcc cggcggcgtg
       61 cgtttgccct tgccgagttc ggatttgagt gtactcgtgg tgacgccgga atgaattcga
      121 agcagcgcgt gctggcgaca ctagcgggcg agctgccgga tcgcgtgccc atcggcgaat
      181 tcgcagtgga ctttgatacg gtcgagcgtc tgctggggca tgaaacctat ttgcgcgcca
      241 aggcgaaatc gcagatcgcg ttctgggaag ggcggcacgc ggaagtggcc gaaagttata
      301 tcaaagacca catcgcgctg cacgagacac tcgaactgga cattgtgacc tttccggccg
      361 cgacctggcg cattcctccc gaaactgatg atgcgccgcc gcgccgcacg agtccggaca
      421 cgtgggaaga caagtacggg cgggtttacc gctattctgc ggcgtccgaa gacataactt
      481 gtatccatga tccggtggcg gacgccgaga cgttgtccgt ggcagatttc gagggcccgc
      541 tgcggccgcc agcaattgac gcacgctcgt ggcacatact cgacaaggtg atccagcgct
      601 tcaaagacga aaagttcatc tgcgggccgt cgggcggaga gatcggcatt gttctgctgg
      661 gcgggctgac gcgcggatgc aggcgcttgg cgcggcagcc ggaggtggtg cgcgcggcga
      721 cccgccggat gctggaggag cagaatctcg cggacgagca gcttgtacac gccgactgcg
      781 atggcgtgct ctgggatcag gactttgcat acaatgccgg accgttcatc agtccggccg
      841 tgtttcgcga attattcctg ccagcgaatc ggcagcgcgt ggcgcgactg cacgaggttt
      901 tcggcaagaa ggttctgaag cactgctgcg gcaacatccg ggcgttgcag gactgcttcc
      961 tggagatagg ctacgacgcg tatcagtcga ttcaggccag cgccggcatg gatctcgcca
     1021 cgttgaagag ggaaatcggg gggaaaatga cgctttgggg cggggtggcc gtcgaacacc
     1081 tgatttctgg ggctccggaa gacgtgcgcg acgatgtgcg gcgcgctatg gcctgcggca
     1141 agccgggcgg gcgtttcata ctgggcgcga gccactcgat tgccgtcggc acgcgttatg
     1201 acaattacat ggcgatgttg gacgaatacc acaggttgtg cgagtactag acatgcggcg
     1261 agctgcgcaa aaggaaccgg ctgaacgacc gaggctgaag aggagcgtgc atctgctgtg
     1321 caattcccat gttgatgcgg cctggctttg ggagtggcag gaaggtgcgg ccgaggcgct
     1381 ggcgctggcc cgcacggtcg ctgacatctg cgagaagttc cccggcctgg ttttcaatcg
     1441 caacgaggcg atgttccacg agtggatcgc cgagtacgac ccggcgctat ttcagcgcat
     1501 tcgcaagctg gtgaaaagcg gggcgtggca cattatgggc ggatggtatt tgcagccgga
     1561 ctgcaatata cctggaggcg aatcgtttgt gcggcagatc ctggtgggaa agcgttactt
     1621 caagcgcgtc tttggcgtgg acgtcaagac ggcggcgaac ctggattcgt tcgggcacag
     1681 tcgcgggttg gtgcagatac tggcgcgcag cggatacgac tcatacctgt tctgtcgacc
     1741 tccccagcgg gatgcggact tgccggccac gcagttcgtc tgggtcggct acgacggttc
     1801 gcaggtgctg gcgacgctgg cggactcgca ctacaactcg gcgccgggcg gcgcccgcca
     1861 gaaggtggtg gattggatag gcgcaaatgg gggccaggcc tgcggaatcg tgccgtgggg
     1921 cgtcggcaat cacggcgggg ggccgtcgcg caaggacgtc tctgaactga acgcattgat
     1981 gcgggagatg cccgatgtgg acatccggca ttcgacgcct gagcagtatt tcgcagacct
     2041 gcggcgccgg cgaagtgagt tgccgcagca tgggggcgat ctgaacccct gggccgtggg
     2101 cgcctatacg tccatggcgc gcgtcaagca gaagcaccgc aagctggaaa acgagctgta
     2161 tgccacggag aagatggccg ccacggctgc catacagggc ctgatgcgct atccgcggac
     2221 ggagttttac gaggccacgc gcgacctgct ggcgagcgag tttcacgact cactggcggg
     2281 aactgtgatt gagccggtgg aggacgcagt aattcagcgg ctggaccacg gtctggaagt
     2341 tgtgtcgcgc atcaagatgc gggcgttcct gacgctggcg gccggccagc cggcagcgcg
     2401 gccggacgaa ttcccggtac ttgtgtacaa cccgcacccg tatccggtgc gcacattggt
     2461 cgaatgcgaa atgcaaacgc cttggccgca caggactggg cgatttggcg ttccgcaggt
     2521 gcggcagggg cgtcgtctgt tgcctgcgca ggccgagaaa gaacacgcca acattaatga
     2581 ggaccatcgc aagcgggtcg tgtttgcggc tgaactggcg ccgagccaaa tgaatcgttt
     2641 cgactgccgc gtcgtactgc agcccaagcg tccggccttt ggcctcaagg aaagccgcgg
     2701 ggtgattcgg ctcaagacgg cgtccatgga agttgtgatt aacacgcgca ccgggctgct
     2761 cgaccgctat cgcgtcggag gatgcgacta cctggcgcgc ggcgcgttcc gaccactggt
     2821 aatgcaggac gacgccgatc cgtggggcat gagggtgcgg cgttttcgca cgccggccgg
     2881 gcgttttcgc ttgatgtcgc gggtccgcgg ggcgcgcttt tcggccgtgg gcgacgagca
     2941 tctgccgtgc gtgcgggtaa tcgaggacgg accagtacgc agtgtggtgg aggtgctgct
     3001 ggaatatgaa gactcctgta tttgccaacg atacaagttg ccaaaggcag gtacggagat
     3061 tgaggtcgag gcgcgcgtcc actggtacga gaaagaccgt atgctcaagc tgtctatccc
     3121 cacgctcgtt ccaagccccg catttgccgg tcagacagcc ttcggcgtcg cggagctgcg
     3181 cgccgatggc gatgagcagg tgatgcagaa atggctcgcg gtcacctcga aagacagcga
     3241 tatggctttg acaatcatca acgaccgcat acacggggcg gactgcgcgg acggcgaagt
     3301 gcggctgtcg ctgctgcgtt cacccgcaca cgcggggcat ccgctcggca acgggcggcc
     3361 gatcacgaaa caggaccgct tcacgccgcg catagatcag ggcgagcacg tttttcggtt
     3421 ctggctgaat gccggagcgg cacaagagcg cttggcgcac gtcgaccgtg aggcgctgtg
     3481 gcacaatgag cagccgtacg cgctctgcgt atctcctggt gtgccggaac gggcggcgcc
     3541 ccgtcctggt gtcgtcctta gcgacgacgt tatccagatg agcgccatga aactggccga
     3601 ggacaacaac gatttgatca ttcgcctttt cgagccgacg gggcgaaagc gcactacgcg
     3661 tctgtcactg cctggggcgg gcgtccaggc caagctggca ttcgtgcctt ttgagatcaa
     3721 gtcgctgcgc gtcaacagca gaaccggggc cgtgagagaa gtcgatttgc tcgagaagta
     3781 agaggttgat gacaatgccc ggaccagttt ccattgtgct ctcaggactc ggcggctacg
     3841 gactgtgtta tctggaaggg cttctggatc agccggacga cggccggttt cgcctggtag
     3901 ccggcgttga cccggctccg cagaactgta agaggttggc cgatctcgaa gcgcggcgcg
     3961 tgcctgttta cccgtcactg gaagagttct accgtgtcgg cagggcggac ctggcagtgt
     4021 tgtcctcgcc gattcaccag cattgccggc agacgtgcct ggcgctggcg cacggcagcc
     4081 atgtgctgtg cgagaagccg gccgcggcca cggttcagga cgtcgaccgc atgattcggg
     4141 cgcgcgacga ggccaggaaa tcagtggcga tcggctacca gtggtcattc tcaaccccga
     4201 ttcagcggtt gaagcgcgac atcctggccg gagagtttgg ggcgccgcgg cggctgcgta
     4261 gtgtgtgttt gtggccgcgc gatgaagtct actacacgcg taatgactgg gccgggcggc
     4321 agcgtgcggc agacggttcc tggattctcg acagtccggt caacaatgcc atggcgcacg
     4381 acctgcacaa catgctctac ctgctggggc cgagcattga tcgcagcgcg cagccggtcg
     4441 agatcgtcgc cgaactgtac cgcgccaatg acattgagaa tttcgacacc gccgcgttgc
     4501 gcgtccgcac agggagcggc gccgaagtcc tgttctatgg gtcgcatgcc atcgcggagg
     4561 actggggccc cgagttctgc tttgagtttg aacgggcgac ggtgacgtat gtgacgaacc
     4621 gtgagccgat cgtggcgcgg tttcg
//
LOCUS       DAOSYS010000273         4639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15298_length_4639_cov_5.426920, whole genome shotgun sequence.
ACCESSION   DAOSYS010000273 DAOSYS010000000
VERSION     DAOSYS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15298_length_4639_cov_5.426920"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLU99_13665"
     CDS             complement(<1..1239)
                     /locus_tag="PLU99_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOO18137.1"
                     /translation="MSRFHLLIAAVWITAAGSLCLAQAPAASTQPPPETQEQRDERMR
                     WWREARFGMFIHWGLYAIPAGQWGDETGHGEWIRDSARIPIEEYDQLVEQFNPVRFDA
                     EAWVRIAKDAGMKYIVITSKHHDGFCLFDSKHTDFDVISTPFGRDILHELAVACRKEG
                     LRLCWYHSIMDWHHPDYLPRRAWEERSAEGADFSRYYAYLKDQVRELLTHYGQIGVLW
                     FDGEWEPTWTHEYGRALYDYVRSLQPGIIVNNRVDKGRGGMAGLTSGDHAGDFGTPEQ
                     EVPATGLPGVDWETCMTMNDHWGYNSHDQNFKSTRDLLHTLADAASKGGNFLLNVGPT
                     AGGEFPPECVERLREIGQWMKVNGEAIYGTQAGPLKTPTWGRCTQRRLDGDVTRLYLH
                     VFDWPTDGRLIVRDLLSQPAN"
     gene            complement(1656..2528)
                     /locus_tag="PLU99_13670"
     CDS             complement(1656..2528)
                     /locus_tag="PLU99_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18138.1"
                     /translation="MKAVTIVAVLICTSVAWATPTVTLTLESSKNGLTVSPGTTVDWS
                     VKVAVSPGDNAGLALVSTDLIQNPDNPALFDIPQGSPDSIDATMQNFNRPAGIANPGE
                     GGATTGYIGSQRGEAGQKNLIQIGGGQNTFGAALPSGTGIGESATVLPGVGQGAEPQL
                     VLSGSFVAPAVPGTYTFHLENGLANVLNNISPPPVPPEFWTVTAATVELGTASFTFTV
                     GGASLGDVNCDGQVNVFDIDPFVLALTNPEQYALMFPACHISSADANCDGEVNVFDID
                     PFVQCLTSGCAPCP"
     gene            2978..3598
                     /gene="cysC"
                     /locus_tag="PLU99_13675"
     CDS             2978..3598
                     /gene="cysC"
                     /locus_tag="PLU99_13675"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOO18139.1"
                     /translation="MSKQVATNITWHEGHVDRAQRNELLRQKGATIWLTGLSASGKST
                     IAFTAEHALVERGHLSYVLDGDNIRHGLNKNLGFSPADRTENIRRIGEVAKLFADAGV
                     LTFTSFISPYRADRDLVRQLHEAAGLPFIEIYVQASVELCEQRDPKGLYKKARAGEVK
                     EFTGISAPYEEPLKPEMVIDSANVSPQQAAGLIISCLEERGLLPRQ"
     gene            3636..>4639
                     /gene="sat"
                     /locus_tag="PLU99_13680"
     CDS             3636..>4639
                     /gene="sat"
                     /locus_tag="PLU99_13680"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOO18140.1"
                     /translation="MSDARLIPPHGGKLVNLIVDEGQRRALSEQAKGALRVRLPEREQ
                     CDLELLAVGAFSPLTGFMGEAEYHRVLDEMKLSNGLPWTVPITCSVDGATAERIAVGQ
                     PVALTDDQDRTLAVLTVREKYAHDKKKEAERVYQTTDEKHPGVAVTMSQGDVCLGGPV
                     QVICPRYEPEFARFRLTPAQTRAAFSERGWRTVVAFQTRNPIHRAHEYITKCALEICD
                     GLLIHPLVGQTQAGDIPAEVRMRCYEVLLANYYSPKNTLLSVWPAAMRYAGPKEAILH
                     ALVRKNYGCTHFIVGRDHAGVGTYYGTYDAQRIFEKFDPAAIAIQPLNFEHTFWCKKS
                     G"
BASE COUNT          858 a         1441 c         1490 g          850 t
ORIGIN      
        1 attcgcgggc tggcttaaca ggtcgcggac gatcaggcgg ccgtcagtgg gccagtcgaa
       61 aacgtgcaga tatagccggg tcacgtcgcc gtccagccgt cgctgcgtgc agcggcccca
      121 ggtgggggtt ttgagcgggc cggcctgcgt gccgtagatc gcctcgccgt tgaccttcat
      181 ccattggccg atttcgcgca gccgctccac gcattccggc ggaaactcgc cgccggcggt
      241 cgggccgacg ttgagcagga aattgccgcc cttggaggcg gcgtcggcca gcgtgtgcag
      301 caggtcccgc gtcgacttga agttctggtc gtgactgttg tagccccagt ggtcgttcat
      361 cgtcatgcag gtttcccagt ccacgcccgg caggccggtg gcggggactt cctgctcggg
      421 cgtgccgaag tcgccggcat gatcgccgct ggtcaggccc gccatgccgc cgcggccctt
      481 gtcgacgcgg ttgttgacga tgatgccagg ctggagcgag cgcacgtagt cgtacagggc
      541 ccggccgtat tcgtgcgtcc aggtcggctc ccattcgcca tcgaaccaga gcaccccgat
      601 ctggccgtaa tgggtgagca gctcgcggac ctggtctttg agatatgcgt aatagcggct
      661 gaagtcggcg ccttcggccg agcgctcttc ccaggcacgg cgtggcaggt agtcaggatg
      721 gtgccagtcc ataatcgagt ggtaccagca cagccgcagc ccttctttgc ggcaggcgac
      781 cgcaagctcg tgcaggatgt cgcggccgaa gggcgtggat atcacgtcaa agtcggtgtg
      841 cttcgagtcg aacaggcaga agccgtcgtg atgtttgctg gtgatcacga tgtacttcat
      901 gccggcgtct ttagcaatgc ggacccaggc ttcggcgtcg aacctgaccg ggttgaactg
      961 ctcgactagt tggtcgtatt cttcgatcgg gatgcgggcc gagtcgcgaa tccactcacc
     1021 gtggccggtt tcgtcgcccc actggcccgc gggaatggcg tacagccccc agtggatgaa
     1081 catgccgaag cgggcctcgc gccaccagcg catgcgctcg tcgcgttgtt cctgtgtttc
     1141 aggcggaggc tgggttgatg cagccggcgc ttgtgccagg cagaggcttc ctgcggctgt
     1201 tatccaaacg gctgcgatca gcagatgaaa tcggctcatg tgacacctcc ttggcggcgc
     1261 agaataggct ggggttgctt gggctgcaat gcggtaaagg gtcgcgggtg ccgatgaaac
     1321 cgcactgctt gaagctgcgc tttcaagcag tggcacactg gggcttcaag caggggcatc
     1381 tctcttgaag gagcgagccg tcgtcaagtg cgttggaagc cgctaacgag ggcatgccgg
     1441 cgccgctgcg cggcttgggc atggcacccg gcgaagcttc actgcttgaa gctgcgcttt
     1501 caagcagtgg cacaccggcc ctttatcacc ctatcacttt atcaccttat cacttcacga
     1561 cgggcgcgcg ggctgggcct aacaagaatc agggcctcgc gtgtgcgagg ccctgtttct
     1621 gcggtttcgc cctcaaacta cgcaaattcg tgttactacg gacaaggcgc gcagccactc
     1681 gtcaggcact gcacgaacgg gtcgatgtcg aacacgttga cctcgccgtc gcagttcgcg
     1741 tccgcactgc taatgtggca agcggggaac atcagcgcgt actgttccgg gttcgtcaac
     1801 gccagcacga acggatcgat gtcaaacacg ttcacctgcc catcgcagtt cacatcgccg
     1861 agtgacgcac caccaacagt aaacgtgaag ctcgcggtcc ccagttcaac cgtcgccgca
     1921 gtgaccgtcc agaactccgg cggtaccggc ggcggtgaga tgttgttcag tacgttggcc
     1981 aagccgttct ccaagtggaa cgtgtacgtg cccggcacgg ccggcgcgac gaatgagccg
     2041 gacaacacaa gctgcggctc ggcgccctgg ccgacgcccg ggagcacggt ggcgctttca
     2101 ccgattcccg ttccggacgg cagggccgcc ccgaacgtgt tctgcccgcc gccgatctgg
     2161 atcaggttct tttggccggc ttccccgcgc tgcgagccga tgtagccggt ggttgccccg
     2221 ccctcgccgg ggtttgcaat accggcgggc cggttgaagt tctgcatggt ggcatcaatg
     2281 ctgtccggcg aaccctgcgg aatgtcaaac agggcgggat tgtcggggtt ctggatcaga
     2341 tcggtgctga ccagggccaa accggcattg tcgccgggcg agaccgcgac cttcacgctc
     2401 caatccacgg tcgtcccggg tgaaaccgtc agcccgtttt tgctcgattc gagggtgagc
     2461 gtcacggttg gcgtggccca cgccacgctc gtgcagatca atacagctac tatcgtgacc
     2521 gccttcatgg tgagcacctc ctgatggggc gtacgccccc gcacagaatg tggaattttg
     2581 gcttcaatcc tattgcatgc cctcgcgaac agtgagcatc acggtggcca ttttaacgcg
     2641 ccggccgcgc cgcggaaact gttttctcat ctgcgccgga aatttgcaca gttttcggac
     2701 tcaaatctcg cagaaatggc tttcgtccgg tgtaagtgct ttgcacgcca aagcttagag
     2761 ccgttgtggg ccgcgggcaa aacgtgcgcc gcggagtaga ccaatgctcc gcgggcactt
     2821 atcctcgcct ctgtggcacg cgcgctgcca aagaacgacc aattggagcc agagcgacgg
     2881 ccgggagaaa cgcgctctcg agccgaaacc gagcgctggc gacgcgttgc gagcgcgttt
     2941 ggcgcgtagc tttgtccact atttgttggg atgacgcatg agcaaacagg tggcaacgaa
     3001 tatcacatgg catgagggac acgtcgatcg cgcgcagcgc aatgagctgt tgcggcagaa
     3061 aggggccacg atctggctga ccgggctaag cgcttcgggc aagagcacga tcgccttcac
     3121 cgccgagcac gccctggtcg aacgcgggca cctcagctac gtgctcgatg gcgataacat
     3181 ccgccacggc ctgaacaaga acctgggctt ctcgcccgcg gaccgtaccg aaaacatccg
     3241 ccggattggc gaggtcgcca agctcttcgc cgacgcgggc gtgctgacgt tcacgagctt
     3301 catcagcccg tatcgggccg accgcgacct ggtgcggcaa ctgcacgagg ccgccggtct
     3361 gccgttcatt gaaatatacg tccaggcgtc agtcgaactc tgcgaacagc gcgacccgaa
     3421 ggggctgtac aagaaagccc gggccggcga agtgaaggag ttcacgggaa tcagtgcgcc
     3481 ctacgaggag ccgctcaagc ccgaaatggt catcgattcg gccaacgtct cgccccagca
     3541 ggcagccggg ctcatcatta gttgcctgga agagcgcggg ctgttgccgc ggcaatgatc
     3601 cgggggcggg ttgattggaa aggactgcca agaaaatgag tgatgcacga ctgattccgc
     3661 cacacggcgg caaattggtg aacctgatcg tcgacgaggg acaacggcgc gcgctgagcg
     3721 agcaggccaa gggcgcactg cgcgttcgcc tgccggaacg cgagcagtgc gacctggaac
     3781 tgttggccgt gggcgcgttc tcgccgctga ccggcttcat gggcgaggcg gaataccatc
     3841 gcgtactgga cgaaatgaag ctgagcaacg ggctgccctg gacggtgccg atcacctgct
     3901 cggtggatgg cgccactgcg gagcgaatcg ccgtcggtca gccggtcgcg ctcaccgacg
     3961 accaggatcg caccttggcg gtcctcaccg ttcgcgagaa gtacgcgcac gacaagaaaa
     4021 aggaagccga gcgcgtctat cagacgactg acgaaaagca ccccggcgtg gcggtcacca
     4081 tgtcgcaagg cgacgtatgc ctgggcggcc cggtgcaggt gatctgtccg cgctacgagc
     4141 cggaattcgc ccgcttccgc cttaccccgg cccagacgcg agcggccttc agcgaacgcg
     4201 gctggcggac ggtggtcgcc ttccagaccc gcaacccgat ccaccgggcg catgagtaca
     4261 ttaccaagtg cgccttggaa atctgcgacg gtctgctgat ccatcccctg gtggggcaga
     4321 cccaggccgg cgacatcccg gccgaggtgc gcatgcgctg ctacgaggtg ctgctcgcca
     4381 actactacag cccgaaaaac acgcttctat ccgtctggcc ggccgccatg cgctacgccg
     4441 ggccgaagga agccatactg cacgcgctgg tccgcaagaa ttacggctgc acgcacttca
     4501 ttgtcgggcg cgatcacgcc ggggtcggga cgtactacgg cacgtacgac gcgcagcgca
     4561 tcttcgaaaa gttcgacccg gcggccatcg caatccagcc gctgaatttc gagcatacgt
     4621 tctggtgcaa gaaatccgg
//
LOCUS       DAOSYS010000274         4639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15299_length_4639_cov_5.262653, whole genome shotgun sequence.
ACCESSION   DAOSYS010000274 DAOSYS010000000
VERSION     DAOSYS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15299_length_4639_cov_5.262653"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLU99_13685"
     CDS             complement(<1..628)
                     /locus_tag="PLU99_13685"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOO18141.1"
                     /translation="MRFLLAPDKFKDALDAAGVCQAMAAGIRQVLPHAEIITCPMGDG
                     GEGTGPLLAQALDASEQRETVLDALGRECTARWWLTADRTTAVIEMAEASGLWRLSPA
                     ERGALRTSSHGTGQLIKAASESGARRVFLCVGGSATVDGGAGCLQALGWRLLDAGGRE
                     LSPPLTGADLRRIARLEPPANPPVPPNPPVIDLQILCDVDNPLLGPRGA"
     gene            complement(625..1440)
                     /gene="lpxA"
                     /locus_tag="PLU99_13690"
     CDS             complement(625..1440)
                     /gene="lpxA"
                     /locus_tag="PLU99_13690"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOO18142.1"
                     /translation="MAIHPTAVVDRRAEIHPEAEIGPYAIIEGDVRIGAGTRIEAHAF
                     VGEGTTIGRGCRIHPFAVVGHSPQDLKYRGGVSYTQIGDETVIREHAQIHRGVAPGSS
                     TIVGCRCLIMATAHVGHNSVVGDEVKMANGALLAGHVEVGNGVFMSGNAVAHQFSRIG
                     ELVMVGGVARVPRDVPPFMSVVYEEIYGINVVGMRRAGFTREERDEIKRCYRILFRSS
                     LAFPAAIAQVVETVQTGPGRRLAEFLQAPSRRGYMHGPRRTRRLEAAIDQEVS"
     gene            complement(1493..2248)
                     /locus_tag="PLU99_13695"
     CDS             complement(1493..2248)
                     /locus_tag="PLU99_13695"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOO18143.1"
                     /translation="MNKLGVNIDHVATVRQARRTDEPDPIWAAVEAELGGADCITFHL
                     REDRRHINDRDAELLKATVATKLNMEMSINPGIVKVALRLKADQCTLVPEHRAELTTE
                     GGLDVVGGRRRIKPVVKRLKDAGIITSAFIEPHPEQIAAAAECGFQAIELWTADYANA
                     KTERARTQALQRLMQGIESGLDHGLTVHGGHGLTYRNVGPVAELGGFEEFNIGHSIVA
                     RAIFVGMREAVREMKRLLDRATARIAIIDSEPE"
     gene            2834..3709
                     /locus_tag="PLU99_13700"
     CDS             2834..3709
                     /locus_tag="PLU99_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18144.1"
                     /translation="MSFREKVLITCIAVLLAGNAFAPAVHAESVLYTPSNADNPSFRS
                     ALAALIGGPVDYFDSRVAVPTLDELLQYDCVFTWVNYPYADDEEMGNVLADYVDAGGR
                     VILGQWTWSGLYGSPATGPSRILQPEYCPVEVVFTGFGSGTYAGDGSDCVHVAVGSYQ
                     TDYLDIIGALNLGAQSDGTFEPSGTPAVAWRPDHRVYYSPGNTGGAYGTGDWVQLTAN
                     MITCRPAPVTGDLNCDGAVNVFDIDPFVLALTDQAAYGAQFPYCDYMLADSNGDGSVN
                     VFDIDPFVALLVGAP"
     gene            3891..4448
                     /locus_tag="PLU99_13705"
     CDS             3891..4448
                     /locus_tag="PLU99_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18145.1"
                     /translation="MARQPEDVKACEVCGASIYPEHVKKGLADYLAGRLLCSHCLREK
                     KQIAMVNPGAVFRDEQAQPDEDEPIALAGEDEYEIYSPSPAASGSGSLRGGSAAGLSG
                     GSTFGGAALERLYKRDLLKGPNATRCRTFHCKLNDSSFARLNEVINDWVDANEDVEIK
                     FATSCIGGLEAKHSTDPHLIITVFY"
BASE COUNT          816 a         1504 c         1477 g          842 t
ORIGIN      
        1 cagcgccgcg cggtccgagc agggggttat ccacgtcgca caaaatctgc agatcaataa
       61 ctggcggatt gggcgggact ggcggattgg ccggcggttc caagcgcgcg atgcgccgca
      121 aatccgcgcc tgtaagcggc ggcgacagct cacgcccgcc ggcatcgagc aagcgccagc
      181 cgagggcctg aaggcagccg gcgccgccgt cgaccgtcgc cgagccgccc acgcacagga
      241 atacccggcg cgctccgctt tccgaggccg cctttatgag ctgccccgtc ccgtgcgaac
      301 tggtgcgcaa cgcccctcgt tcggcaggcg aaagacgcca caagcccgac gcctcggcca
      361 tttcaatcac ggccgtcgtg cggtccgcgg tcaaccacca gcgggccgta cactcccggc
      421 ctagagcgtc gagcacggtt tcgcgttgtt cgctcgcgtc cagcgcctgg gccagcagcg
      481 gcccggtgcc ctcgccgcca tcgcccatgg ggcaggtaat tatttcggcg tgcggcagga
      541 cctggcgaat tccggccgcc atcgcctgac agacccccgc ggcatcgagc gcgtctttga
      601 acttgtccgg cgcgagaagg aatctcacga aacttcctgg tcgatcgccg cctccagccg
      661 gcgcgtgcgg cgcgggccgt gcatatagcc gcggcgcgac ggcgcctgaa ggaactcagc
      721 caggcggcgc ccggggccgg tctgcacggt ttcgacaact tgggcgatgg ccgccggaaa
      781 ggccaggcta gagcggaaca gaatgcggta gcagcgcttt atttcgtcgc gctcctcgcg
      841 cgtgaacccg gcccggcgca tgccgaccac gtttatgccg tagatttcct cgtagaccac
      901 gctcatgaac ggcgggacgt cgcgcggcac gcgggccacg ccgccaacca tcaccagttc
      961 gccgatgcgc gagaactgat gcgccacggc gttaccgctc ataaacacac cgttgccgac
     1021 ttcgacatgc ccggccagca gagcgccgtt ggccattttt acctcgtcac ccaccacgct
     1081 gttgtggccg acgtgcgcgg tggccatgat caagcagcgg cacccgacga tagtggaact
     1141 gccgggggcg acgccgcgat ggatttgggc gtgctcccgg atgacggttt catcgccgat
     1201 ctgggtataa ctcacgccgc cccggtattt cagatcctgc ggcgagtggc cgacgacggc
     1261 gaacggatgt atccgacagc ctcggccgat ggttgttcct tcgccgacga aggcatgtgc
     1321 ttcgatgcgc gtgccggcgc cgatacgcac gtcgccctca atgattgcgt acgggccgat
     1381 ttcggcctcc gggtgaattt cggccctgcg gtctacaact gcggtcggat gaatcgccat
     1441 tgacgttgct acctcacgtg ttacgtgtcg cgtcaggttt tctggacccg atctactcgg
     1501 gttcggaatc gatgatcgcg atgcgtgcgg tggccctgtc caggaggcgc ttcatctcac
     1561 gcaccgcttc gcgcatacca acgaaaatcg cgcgggccac aatgctgtgc ccaatgttga
     1621 attcctcaaa gccgcccagt tcggccaccg gaccgacgtt acggtaagtc agcccgtggc
     1681 cgccatgaac cgtcaggccg tggtcaagcc cggattcgat cccttgcatc agcctctgga
     1741 gcgcctgcgt gcgggcgcgc tcggttttgg cgttggcgta atcggccgtc cacagctcga
     1801 tggcctggaa accgcactcg gccgcggcgg cgatttgctc cgggtgcggt tcgatgaagg
     1861 cgctggtgat gatgcccgcg tctttcaggc gcttgacgac gggcttgatg cggcggcgcc
     1921 cgccgactac gtccaggccg ccctcggtgg tcagctcggc ccggtgctcg ggcaccaagg
     1981 tgcactgatc ggccttcaag cgcagcgcga ccttcacgat gccggggttg atcgacatct
     2041 ccatattgag cttggtggcc accgtcgctt tcagcagttc ggcatcgcgg tcgttgatgt
     2101 gccgccggtc ctcacgcaga tgaaacgtga tgcagtcagc cccgcccagc tccgcctcca
     2161 cggccgccca gatcgggtcc ggctcgtccg tgcggcgggc ctgccgcacg gtggcaacat
     2221 ggtcgatgtt cacgcccagc ttgttcatgc agcctccaat atacaggcgg gtatcctacg
     2281 gaaatgcccc gccgggtgcc aatgccggat aaagccccgc gacgcgttgg gtgccatgct
     2341 caagccgcgt agcggcgcgg gcatgccgtg gccggtaacc cccggcacct ggcgccccca
     2401 aggcggagca tgcccgtcgt taaagaaggc acgtccgccg ttaacgaggg cacgtccgcc
     2461 gttaacgcgg gcatgcccgc gcttacagct tgggcatggc acccaaggcg cgattgctgt
     2521 aagtaactgg aggcacgtta cttgcgcgcg gctgtggccc gactgcggca agggcgaacg
     2581 cccaacactg gcggacgcgc caaaatcgcc tttgcgtcgc tgtctttgta tcgctcaagc
     2641 aaaaaaaggt cttcatgcaa cgccggcggt atgttatact gacgtagaca gttggttggt
     2701 atctgacgtg ggcaattggc cggttatccg gcgtacgcga acggcggggc tcgctgtccc
     2761 gtcttccgat tctcggcagc aacgtctcgt gccataagac gagcacagtc cagttttcga
     2821 aggcggaagg atcatgagtt ttcgagagaa agtcctgatc acgtgcattg cggttttgct
     2881 tgcgggcaac gccttcgccc cagccgtgca tgccgaaagc gtgctctaca ccccgagcaa
     2941 cgcggataat ccgtcgttcc gcagcgcact ggcggccctg atcggcggac cagttgatta
     3001 cttcgattcc agagtggccg tgcccacact ggatgagcta ctgcagtacg actgcgtctt
     3061 cacctgggtg aattaccctt atgcggacga cgaggaaatg ggcaatgtgc tggcggatta
     3121 cgtggatgcc ggaggacggg tcattttggg acagtggacc tggtctggat tgtatggatc
     3181 gccggctacc ggtcctagtc gaattctgca gccggagtac tgccccgtcg aggtcgtgtt
     3241 taccggcttt ggatcgggca cttacgccgg agatggctcc gactgcgtgc atgtcgcagt
     3301 gggtagctac cagaccgact acctggatat aatcggcgcc ctcaatctcg gcgcacagag
     3361 cgacggcacc ttcgagccca gcgggacgcc cgcggtggcc tggcgccccg atcaccgcgt
     3421 gtactacagc ccaggcaata ccggcggcgc gtacggcacg ggcgattggg tgcaattgac
     3481 ggccaacatg atcacgtgcc ggcccgcgcc cgtcaccggc gacctgaact gcgacggcgc
     3541 cgtcaatgtg ttcgacatcg acccgttcgt gctggcactg accgaccagg cggcttatgg
     3601 cgcgcagttt ccgtactgcg attacatgct cgcggactcc aacggcgacg gatcagtgaa
     3661 tgtgtttgac atcgatccgt tcgttgcgct gctggtcggc gcgccctgac ggccggcacc
     3721 gcccggcttc tgcccgaaaa aaaatgccgc gccgcccggc acgccgcgct cggtcctctg
     3781 gttgcaagcc gggcggtcag ccgatatacg gaagtgggcc gaagccgttg aagcaaaaag
     3841 tcagaattgt ccctgggccc cagttgcgcc aggcggcgga ggtagcccaa atggccagac
     3901 agccggaaga tgtaaaagct tgcgaggttt gcggggccag catctacccc gagcacgtca
     3961 agaagggttt ggcggactat ctggccggcc gcctgctatg cagccattgc ctgcgcgaga
     4021 aaaagcagat cgccatggtg aacccgggcg cggtgttccg tgacgagcag gcccaaccgg
     4081 atgaggatga accgatcgcg ctggcgggcg aagatgagta tgaaatttac tcgccttcgc
     4141 ccgccgcgag cggaagcggc tcccttcgcg gagggagcgc cgcgggactg agtggcggca
     4201 gcaccttcgg gggtgcggcg ctggagcgcc tttacaagcg cgacctgctc aaaggcccca
     4261 acgctacccg ctgccgcacg tttcactgca agctcaacga ctcctccttc gcgcggctga
     4321 acgaggtgat taacgactgg gtcgacgcca atgaggacgt cgagatcaag tttgccacca
     4381 gttgtatcgg cgggctggaa gccaaacact caaccgaccc gcacctgatc atcacggtgt
     4441 tctattgaaa cgcgggggcg catgaagctg ctacgttttc tcgacgggca ggggcgaatc
     4501 tgcctggggg agccggcagg accccggctg gcgcgcccgc tgatttccgc gccgggcgat
     4561 ccggccgtct tcgcgcctca ccccgttacg attcaaacct acctgccgcc ggtcgagccg
     4621 cccaacatct tcgccattg
//
LOCUS       DAOSYS010000275         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15420_length_4601_cov_5.057853, whole genome shotgun sequence.
ACCESSION   DAOSYS010000275 DAOSYS010000000
VERSION     DAOSYS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15420_length_4601_cov_5.057853"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLU99_13710"
     CDS             complement(<1..471)
                     /locus_tag="PLU99_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO18146.1"
                     /translation="MRRRADQSRFKAMTLLEVVIAVSLIAVLMGALFTFYWQSLEIRN
                     QVGRIADRTEIARQVLGRLAAELRGCVGKETFGFPLEQRLVGDRRSITFLTTTLPEEH
                     QYEFYSEFDQLPPGQHDLRELKYELWIDPNKKTEDNEPVVGGIVRTEKRTLNQSM"
     gene            complement(471..1472)
                     /locus_tag="PLU99_13715"
     CDS             complement(471..1472)
                     /locus_tag="PLU99_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18147.1"
                     /translation="MKCRTDQGTKANEKRRGRAALLLEVVVAMAIMVGALGVLGAQLV
                     SGLRATEAADLRTRAGALLERVLGLVEYDPELQDRLLTEERFENEFGDDLPGWFYEIE
                     FKPIEEVAGLGQIRISVLYQSEDDARSGAGARLLSQTVLLKAARAKINLVEDFGLPAD
                     KLEEITALLPEGLDPTDFDPQRLIALAKADPQLIISLLPALVPLLQQYFGQGGGLPGD
                     LQLPGGFSPQDLADLEEAAGALLGGGAGGGRGGAGVRPPPGGPPPGASTVTPRAGGRG
                     RGGQSGAGIQSGGTRTGGGRSGRAPQPALRQGSGPGGQYTIEDLMRLREELQSQGGG"
     gene            complement(1469..2227)
                     /locus_tag="PLU99_13720"
     CDS             complement(1469..2227)
                     /locus_tag="PLU99_13720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOO18148.1"
                     /translation="MRSSLARTRRGRPAPAPFTDGAGARRAARRAFTLLEVLLVVSIL
                     AMLAMFTWPTFEGAAERARLDESVRRLKTLVAMCRAEAMSETRCYRITLRLDGSIKLR
                     RQLDPITYPHVYVPVQKDWANLAFMLDGVWVESICLLPDGPPPVEVEDELKTFDHMER
                     DFEPIDIEDFEQPIDITFNPDGTSRSLRWLIRDDTGRGFQLTLDGRLGRLAVEELPAL
                     KPGEAEPPPAINLDQEAELEKAQWEEAGWEPYQP"
     gene            complement(2312..2755)
                     /gene="gspG"
                     /locus_tag="PLU99_13725"
     CDS             complement(2312..2755)
                     /gene="gspG"
                     /locus_tag="PLU99_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOO18149.1"
                     /translation="MSRLIRKRDRRAFTLLEVLMVVVIIGLLAAAIVPSLFGTEEGAR
                     KKLADAAIRAGLGGIDRYRLDMGHFPRSEDGGLTALIEKPSDEQEAKKWNGPYVKPDG
                     LRDPWGNEYVYEYPGRYNEGGYDLSSPGPDGQPGNDDDITNWKVD"
     gene            complement(2787..3995)
                     /locus_tag="PLU99_13730"
     CDS             complement(2787..3995)
                     /locus_tag="PLU99_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO18150.1"
                     /translation="MATFSYRARNATGEVVRGTLVADTTAAAARMLAERSLMPVELDQ
                     VEAAQKSLLTGKARRVKLAKVGMMYEQLADLLRAGVPLLRALTVLSSQSSSPALARVL
                     REVYESVSGGDSLADAMARHPHAFPELHASMVRAGEKGGFLEDVLARLSDFVARQDAL
                     RNKFIGAMIYPCILFVGATGAVIFIMAFVVPKIRPVLEGQVLPLPTRIVFALCDLLQY
                     HYVSALAVLLLGTIVLVGYFQTQAGKQVKNRLQLRAPGIGKIYTMVALCRFCRVFGTL
                     LANGIPILQSLRISKDSTGNALLAEAVEKAAENVRHGEPLAAPLAASKVFPPVIVDMI
                     AVAEESNTLDKVLVEIAKTQEERTAQQIDFAVRMLEPLMLMCMGGVIAFIAVALLLPI
                     MLMATTGLKG"
     gene            4276..>4601
                     /locus_tag="PLU99_13735"
     CDS             4276..>4601
                     /locus_tag="PLU99_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020609913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOO18151.1"
                     /translation="MRQAPPASTNGNAWQELGLQPDLLEGVRLAKFGEPTEIQRELIP
                     VILAGRDCLGQAKTGTGKTAAFALPILQLATAGEPTQALVLVPTRELAAQVDSHIRQL
                     GVRRPL"
BASE COUNT          804 a         1585 c         1403 g          809 t
ORIGIN      
        1 catgctctgg ttgagcgtcc tcttttcggt gcggacgatg ccgcccacca ccggctcgtt
       61 gtcctcggtc ttcttgttcg gatcgatcca gagttcgtac ttcagctcgc gcaggtcgtg
      121 ctgaccgggc ggcagttggt cgaactcact gtaaaactcg tattgatgct cctcaggtag
      181 cgtggtggtc aggaaggtaa tactgcggcg atcgcccacc aagcgctgct cgagcgggaa
      241 gccgaaagtc tccttgccca cgcagccgcg caactcggcc gccagccgcc cgagcacctg
      301 gcgggcgatt tcggtgcggt cggcgatgcg tcccacctgg ttgcgaattt ccaagctctg
      361 ccaatagaag gtaaacagcg cgcccatcag caccgcgatc agcgagaccg cgatcacgac
      421 ctcgagcagc gtcatcgctt tgaagcggga ttggtcagcg cgccgcctca ctaaccacct
      481 ccctggcttt gcagttcttc gcgcaggcgc atcaaatctt cgatcgtgta ttgcccgccc
      541 gggccgctcc cttgtcttag ggccggctgc ggtgcacgtc ccgagcgccc gccgcccgtc
      601 cgggtcccgc ccgattgaat tcccgcaccc gattgtccgc cgcgcccgcg cccgcctgcc
      661 cgtggcgtca ctgtgctggc gccaggcggc ggaccacccg gcggcggtct tacccctgcg
      721 ccgcctctgc cgccaccagc gccgccgccc aggagcgcgc cggccgcctc ctccaggtca
      781 gccaggtctt gcgggctaaa tccgccggga agctgcaaat cgcccggcaa gccgccgcct
      841 tgcccaaaat actgctgcag cagcggcacc agcgccggca ataggctgat gatcaactgc
      901 gggtctgctt tggccagcgc gatcagtctc tgcggatcaa agtcggtcgg gtcaagcccc
      961 tccggcagca gggcggtgat ctcctcaagc ttgtccgccg gcaggccgaa atcctctacc
     1021 aggttaatct tcgcccgcgc cgctttgagc aacacggtct ggctgagcag acgtgcgccg
     1081 gcgccgctgc gcgcgtcatc ttccgattga tacagcaccg agatgcggat ttgacccagc
     1141 cccgcgacct cttcgatcgg cttgaattcg atctcgtaga accagccggg caggtcgtcg
     1201 ccaaactcgt tttcaaaacg ctcctcggtg agcagccgat cctgcagctc cgggtcgtac
     1261 tcgaccagcc ccagcacgcg ctccaacagc gccccggcgc gcgtccggag gtcggcggcc
     1321 tccgtggcac gcagcccact gacaagctgc gctcccaaga cacccagagc gccgaccatg
     1381 atcgccatgg ccactacgac ttcgagcagc agcgcagcgc gtccgcgccg cttttcgttg
     1441 gccttggtcc cttgatccgt tcggcatttc atggctggta cggctcccaa ccggcctctt
     1501 cccactgggc cttttccagc tccgcctcct ggtcgagatt gatggccggc ggcggctccg
     1561 cttcgcccgg cttcaaagcc ggcagctctt ccactgccag gcgtcccaag cggccgtcaa
     1621 gcgttagctg aaaaccgcgc ccggtatcgt cgcgtatcag ccagcgcagc gagcggctgg
     1681 ttccatcggg attgaaggtg atgtcgatcg gctgttcgaa gtcctcgatg tcgatgggtt
     1741 cgaagtcgcg ctccatgtgg tcaaacgttt tcagttcgtc ctccacctcc acgggtggcg
     1801 ggccgtcggg caacaggcag atcgattcga cccacacgcc gtccagcatg aaggccaggt
     1861 tcgcccaatc cttttgaacc gggacgtaga cgtggggata agtgatcgga tcgagctgcc
     1921 ggcgcaactt gatgctgccg tccaggcgca acgtgatacg gtagcagcgc gtttcgctca
     1981 tcgcctcggc ccggcacatg gcgaccagcg tcttcaggcg ccgcaccgat tcgtccagcc
     2041 gggcgcgctc ggcggcgccc tcgaaggtcg gccaggtgaa catggccagc atggccagga
     2101 tgctgacgac gagcagcacc tccagcagcg tgaaagcgcg gcgcgcagcg cggcgggcac
     2161 cagcgccgtc cgtaaacggc gctggtgccg gccgcccccg ccgcgttcgt gcgaggctgc
     2221 ttcgcatgct gttagccacc gggaggtgga agcgcacgcg ccacattcgg gccgcggatc
     2281 tgggagcaca cacggcccgt aggccgcagg gctagtccac cttccagtta gttatgtcgt
     2341 cgtcgttgcc gggctggccg tccgggccgg ggctggataa atcgtagccg ccctcgttgt
     2401 agcggcccgg atactcgtaa acgtattcgt tgccccaggg atcgcgcagg ccgtcgggct
     2461 tgacgtaggg gccattccac tttttggcct cctgctcgtc gctgggtttt tcgatcaggg
     2521 ccgtcagccc gccgtcctcg ctgcgcggaa agtggcccat gtcgaggcgg tagcggtcga
     2581 tgccacccag gccggccctg atggccgcat cggcgagctt cttcctggcg ccttcctcgg
     2641 tgccgaacag gctggggacg atggccgcgg ccagcaagcc gatgatcacg accaccatca
     2701 gcacctcgag caaggtgaac gcccggcggt cccgtttacg aatcaatctg ctcatcgctt
     2761 tctccctaac aagcattttg ggcaggtcat cctttgagcc ccgtcgtggc catcaacatg
     2821 attggcagca gcagggctac cgcaataaag gcgattacgc cgcccatgca catcagcatc
     2881 agcggctcca gcatgcgcac cgcgaagtcg atctgctgcg ccgtgcgctc ctcctgggtc
     2941 ttggcgatct ccaccaggac cttgtccagt gtattgctct cttcggccac ggcgatcata
     3001 tccacaatga cgggcgggaa taccttgctg gctgccaagg gcgctgccag aggttcgccg
     3061 tggcgcacat tctcggccgc cttctccaca gcttccgcca ggagggcatt gccagtcgag
     3121 tctttggaga tgcgcagcga ctgcaaaatg ggaataccat tggccaacag agtaccgaag
     3181 acgcggcaga agcggcacag cgccaccatg gtgtatatct tgccaatacc cggggcgcgc
     3241 aactgcaatc gattcttgac ctgtttaccg gcttgggtct ggaaatagcc aaccagtaca
     3301 atcgttccca gaagcaggac ggcgagggcc gagacgtagt ggtactgcag cagatcgcac
     3361 agggcgaaga cgatgcgcgt cggcagcggc agaacctggc cttccagcac cgggcggatc
     3421 ttgggtacca cgaaagccat gatgaaaatc acggccccgg tggcgccgac gaacagaatg
     3481 caagggtaga tcatcgcgcc gatgaacttg ttgcgcagcg catcctgccg cgccacgaaa
     3541 tccgagagac gcgccagcac gtcttccagg aagccgccct tttcgccggc ccgcaccatc
     3601 gaggcgtgca gctcggggaa agcgtgcggg tgcctggcca tggcgtcggc cagcgagtcg
     3661 ccgcccgaaa cgctctcata aacctcacgc agcacgcgcg ccagagccgg cgaagacgac
     3721 tgggatgaga gcaccgtcag ggcccgcagc agcggcacgc ccgcccgcaa caggtcggcg
     3781 agctgctcgt acatcatgcc caccttggcc agtttgaccc gccgggcctt gcccgtgagc
     3841 agcgacttct gggccgcctc gacctggtcg agctcgaccg gcatgagcga ccgctcggcc
     3901 agcatgcggg cggcggcggc ggtcgtgtcg gccaccagcg tgccgcggac gacctcgccg
     3961 gtcgcatttc gagctcggta ggaaaaagtc gccattattc cgccactatc ccttcaaact
     4021 tcgcgccagc ccaataacat ctattcagga tgcccggttt tatacgccgc ggcggccgtc
     4081 cgggttgcac ctcggccgcc ctggggccgg cgctgtggcc atttcccggc gcgtcctccc
     4141 cggaatcttg tgattcccag gaagttcgct aaattatgac ccctgtcagc gttgatgaga
     4201 agcacgcgcg tcatgaacat ttatcggcac ccgcaacccg acgcgccaga tgagaatccg
     4261 ccaatggcaa aacgcatgcg gcaagcaccg cccgcaagca caaacggaaa cgcctggcag
     4321 gagttgggcc tacagcccga cctgctcgaa ggcgtgcgcc tcgccaagtt cggcgaaccg
     4381 accgaaatcc agcgtgagct gatcccggtc atcctggccg ggcgcgactg cctggggcag
     4441 gccaagaccg gcacgggcaa gaccgccgcg ttcgccctgc ccatactgca actggccacc
     4501 gccggcgagc cgacccaggc cctggtgctg gtgccgacgc gcgaattggc cgcccaggtc
     4561 gacagccaca ttcgccagct cggcgtgcgc cggccgctga a
//
LOCUS       DAOSYS010000276         4566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15576_length_4566_cov_4.669253, whole genome shotgun sequence.
ACCESSION   DAOSYS010000276 DAOSYS010000000
VERSION     DAOSYS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15576_length_4566_cov_4.669253"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..2364)
                     /locus_tag="PLU99_13740"
     CDS             complement(49..2364)
                     /locus_tag="PLU99_13740"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOO18152.1"
                     /translation="MRSNRMFVKSRRAALCGAVLALLAGQALGGTHPRLLISPADVPR
                     LRYACGVATDYDAANGGRPGSHVLEFQALRSHSLGRLADDPLPGEISAVAFLHLIEPA
                     DPLAAQRLALIERTLRQPGPALVSNLEMVLALDWCWADLPPDARRTFITNLAQSAGLL
                     TAADSPLDHRVFREKLAALAAAALIDEEDESSASWGRMRGVILDAGGEYFRKTFPKFL
                     EWRGLAPTSPTAGPNEENDVALAVEIAGLALGTDAWQEFGPRVGRCLEHYVFASFAHP
                     ALQHAFIRDDGTDAPLSPAPRWDELHPLTAHLIALRTGDSAAGFVARRVDQRLRGAAA
                     EPLALPWQWVPIVFDLADVPRCDFSRLPLARNFGGAVVFRSAAGKSETGVWIEAGQPF
                     LRRGQHFDAGHFLIYSGGHLTVSGGDDICLEAVPAKNGEQRLGHSPRPFEFEQYFASA
                     IAHNCLVLQGLLRTERWYGLPYQPLAGQRLIEGTCVDFAGDLDGNPRQTGRQIAYGQN
                     ADAAYLALDLLPAYEPRLASAYTREFLFLDGSALLVVDRLRGAGESDPTWVVHIPARP
                     TLDGSELATAEQIAGDGPDAGIWRADQVAWLRWADGDGVAYMTSLLPNPRKLWIVGGP
                     AKSMTVPQGPHAGRVYSGGDPAGFENLIQPAAKRNPQNAWFRLGQPTVLGSGFAQHAH
                     WGRIEIATAQKANACLFVTLFFIEADRRQAPPVSTEMDGRELRIKFADARPATVALRL
                     DDQLGVTVTRPDGTEWKSPSSIESDASLVEE"
     gene            complement(2448..3053)
                     /locus_tag="PLU99_13745"
     CDS             complement(2448..3053)
                     /locus_tag="PLU99_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18153.1"
                     /translation="MNDSGSILEGVLILLAVLAVVACALTVRRQHSLWPTARRKHAAE
                     SSAAGENADPALGRGLLDLCGRFEELVNRLEAQWEGRISRLEQAVAAVDGRVGQLECL
                     HQPPAVSAVAEPPEPAERPVESPLVYESEPAAVGMVAEPAPESGITSKNLPAADVWPE
                     MQRIYEMADKGVSAIKIAETVAVPLGEVEVALNLRRFAGGA"
     gene            complement(3058..4239)
                     /gene="larC"
                     /locus_tag="PLU99_13750"
     CDS             complement(3058..4239)
                     /gene="larC"
                     /locus_tag="PLU99_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002762763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOO18154.1"
                     /translation="MRCGYFDCFSGAAGDMILAAMIHAGCPLDALRETIARLKLPGVE
                     LRAEVVRRGGLAATHVAVEVAASSHPHHRHLPEITGLIEQAGLAPRVRERALAIFQRL
                     AEAEAAVHGIPIEQVHFHEVGAADAIVDIVGASAGFELLGVEKVVCSPIPTGSGVVVC
                     EHGVLPVPAPATANLLKGAPLAACEEEGELVTPTGAAILTTLAESYGPLPPMRLEAIG
                     YGAGTREGRTRPNLLRLLIGQVEQESTAERDSVAVLEAQVDDSPGQNLAYACERLLRT
                     GALDAYIVPIIMKKGRPGQLITVLCRPEDVNAMERVLFEETTTLGVRRHEATRAKLPR
                     SVVQVETRFGPIRLKVAAAGPGERAWPEYEDCSAAARAGGVSLREVQEEALRVWRAQK
                     S"
BASE COUNT          798 a         1608 c         1405 g          755 t
ORIGIN      
        1 cgcccgtcaa cgccggcgcc gtgcccggcg gcgcgtcagg gccggttact actcctcgac
       61 caggcttgca tcgctctcaa ttgaggaggg cgacttccac tccgtaccgt ccgggcgcgt
      121 aacggtcacg ccaagttgat cgtccagccg cagcgccacg gtcgcgggcc gggcgtcggc
      181 gaacttgatc cgcagctcgc gcccgtccat ctcggttgaa acgggcggag cttgccgccg
      241 gtccgcctcg atgaagaaga gggtcacaaa caggcaggca ttcgcctttt gggcggtcgc
      301 gatttcgatt cggccccagt gcgcgtgttg agcgaaacct gagccgagca ccgtcggttg
      361 gcccagtcgg aaccaggcat tttgcggatt ccgcttggcg gccggctgta tcaaattttc
      421 gaaaccggcc gggtcgccgc cgctgtagac gcggccggcg tgcgggccct gcggaacagt
      481 catgcttttg gccggtccgc cgacgatcca cagcttgcgc gggttgggca gtagactggt
      541 catgtacgcg acgccgtcgc catccgccca gcgcagccag gcgacctgat ccgcccgcca
      601 tatgccggcg tcggggccgt cgcccgcaat ttgttcggcg gtcgcgagtt ccgagccgtc
      661 cagcgttggg cgggcgggaa tgtggacgac ccacgttgga tcggattccc ccgcgccgcg
      721 caggcggtca acaaccaaca gggcgcttcc atccagaaac aagaattccc tggtgtaggc
      781 cgatgccagg cgcggctcat aagcgggcag caggtccagc gccaggtacg ctgcgtccgc
      841 attctgtccg taggcaatct gccgcccggt ctgcctcgga ttgccgtcca gatcacccgc
      901 gaagtctaca cacgtacctt cgatcagacg ctgcccggcc aatggctggt atggcagacc
      961 gtaccagcgt tcggtgcgca acagtccctg caagaccaga cagttatgtg cgattgcgga
     1021 agcgaagtac tgctcgaact cgaacggccg cgggctgtgc cccaagcgct gctcgccgtt
     1081 tttggcgggc acggcttcga ggcagatgtc atcgccgccg gaaaccgtca agtgcccgcc
     1141 gctgtagatc aggaagtggc ccgcgtcaaa atgctgaccg cgccgcagaa acggctgacc
     1201 ggcttcaatc caaacgcccg tctcggattt cccggcggcg ctgcgaaaca ccaccgctcc
     1261 gccgaaattg cgcgccaacg gcaggcgcga aaagtcgcag cgcggcacat ccgcaaggtc
     1321 gaaaacaatg ggcacccact gccaaggcaa agccagcggc tcggccgcgg ccccacgcag
     1381 gcgttggtcg acgcggcgcg cgacgaagcc ggcggctgaa tcgccggtgc gcagcgcgat
     1441 caggtgcgct gtgagcggat gcaattcgtc ccaacgcggt gcgggcgaga ggggggcatc
     1501 ggtgccgtcg tcgcgaatga aggcgtgctg cagagccggg tgcgcgaaag acgcgaaaac
     1561 atagtgttcc aagcagcgtc cgacgcgcgg accgaactcc tgccaggcgt cggtacccag
     1621 cgccagaccg gcgatctcaa ctgccagcgc tacgtcattc tcttcgtttg gtcccgcggt
     1681 cgggctagtc ggcgccaggc cacgccactc caggaacttc gggaacgtct ttcggaaata
     1741 ctcgccgccg gcgtccaaaa tcacgccgcg catccggccc caggaggcgc tggactcatc
     1801 ctcctcgtcg atcagcgcgg ccgcggctag cgcggcaagt ttttcacgaa agacgcgatg
     1861 atcgagcggg ctgtcggcgg ccgtaagcag gcccgcggac tgcgccagat tggtgatgaa
     1921 tgtgcgccgc gcgtcgggcg gcagatctgc ccaacaccag tccagcgcga gcaccatttc
     1981 cagattgctg accagcgccg gccccggctg gcgcagagtc ctttcaatca gcgccagacg
     2041 ttgcgcggcc agcgggtcgg ccggttcgat caagtgcagg aacgctactg cgctgatctc
     2101 gccgggcagc ggatcatcag cgaggcggcc cagtgaatga ctgcgcagcg cttggaattc
     2161 caagacatgc gagcctgggc ggcccccatt tgccgcatcg taatcagtcg cgacgccgca
     2221 ggcgtaacgc aggcgcggca catcggcggg gcttatcaac agacgcggat gggtccctcc
     2281 cagcgcctgg ccggccaaca aagccaagac cgcgccgcag agcgcggcgc gccggctctt
     2341 cacgaacatg cgattcgacc tcacctcgca cacactcctc ctggctactc cataagagtg
     2401 acgtgccgac cgggttgcgc caagcccatg cttccgggcc cggcggctca ggccccgccc
     2461 gcgaaccgcc gcaagttgag ggccacctcg acttccccca gcggaacggc caccgtttcg
     2521 gcaatcttga tggccgatac ccctttatcc gccatttcgt agatacgctg catctcgggc
     2581 caaacgtcgg cggccggcag gtttttcgac gtgatcccgc tttcaggcgc cggctccgcc
     2641 accataccta cggcagccgg ctcactttca taaaccagcg gcgactccac cggccgctcc
     2701 gccggctcag gcggctcggc aaccgcgctc accgcaggcg gctggtgcag acattccagt
     2761 tgtccaacgc gaccatccac cgccgccacg gcttgctcaa gccgcgaaat ccgcccctcc
     2821 cattgcgcct ccaggcggtt caccaattct tcaaaacgcc cgcacaagtc gagtagtccg
     2881 cggcccaacg cggggtcagc gttttcaccc gccgccgaag attcggccgc atgctttctt
     2941 cgtgcggttg gccagagcga atgttgccgg cgcactgtca aggcacacgc caccacggcc
     3001 aataccgcca ggagtatcag aacgccttcg agaatgctcc cgctatcgtt cacgccttca
     3061 gctcttctgc gcacgccaaa cgcgcagggc ctcttcctgc acttcgcgca aggacacgcc
     3121 accggcccgc gccgccgccg agcaatcctc atattccggc catgcacgct cgccgggccc
     3181 ggcggcggcc accttcagcc ggatggggcc aaagcgcgtt tcgacctgca cgacggagcg
     3241 cggcagcttg gcacgcgtgg cctcatggcg ccgcacgccc agcgtcgtcg tctcctcgaa
     3301 cagcacgcgc tccatcgcgt tcacgtcctc cggccggcaa agcacggtta taagctgccc
     3361 cggacgaccc tttttcatga ttatcggcac gatgtacgca tccagcgcac ccgttctcag
     3421 cagccgctcg cacgcgtatg ccaggttctg ccccgggctg tcgtccacct gcgcctcaag
     3481 caccgcgacc gagtcacgct cagcggtgct ttcctgttcg acctgcccga tcaatagccg
     3541 caacaggttc ggccgcgtgc gtccttcgcg cgtgccggcg ccgtagccga tcgcttccag
     3601 gcgcatcggc ggcaacggcc cataactttc ggccagcgtc gtcaggatcg ccgccccggt
     3661 cggcgtgact agctcgcctt cttcctcgca ggcggccagc ggcgcgccct tgagcagatt
     3721 cgcggtggcc ggcgccggca cgggtaaaac gccgtgttcg cagacgacca cgccggagcc
     3781 ggtcggaatc ggcgagcaaa cgaccttttc aacccccaga agctcgaatc cggcggaggc
     3841 ccccacaatg tccacaattg cgtctgccgc gccgacttcg tgaaaatgga cctgttctat
     3901 cgggatacca tggacggccg cctctgcttc agcgaggcgc tggaagatcg cgagcgctcg
     3961 ctctcggact cgcggcgcca gccccgcctg ctcgatcaag cccgtaattt ccggcaggtg
     4021 ccgatgatgc gggtgcgagg acgcggcgac ttcgacagcg acgtgcgtcg ccgccagccc
     4081 gccgcggcgg accacctcgg cgcgcagttc cactcccggg agttttagcc gcgcaattgt
     4141 ctcgcgcagc gcatccaacg gacaaccggc gtgaatcatg gcggccagga tcatgtcgcc
     4201 ggccgccccg ctgaagcagt caaaatatcc gcagcgcacg ttagctcccg attcagtccc
     4261 gtcgcgtccg gaaacactcc gtcatcgact caacagagta ttgcgaaacg ccggtccttg
     4321 cacggggggc gctgcttgcc ggcggggcgg cctgcccaag cgctcagcgc ggtcatgccc
     4381 cgcggacagg ttttcgcgag tggtgttcca gcttcctctg agaatgcaga ttcggcttct
     4441 caccctcagt tattgaaaaa acgcgttcac ggcccctggt tctcgtgcat gcagggacag
     4501 tcggcatccg cctcgcctga cggcgcggca ggggacgggg attcgaatcc gaccagagcc
     4561 tgttcg
//
LOCUS       DAOSYS010000277         4565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15581_length_4565_cov_3.244346, whole genome shotgun sequence.
ACCESSION   DAOSYS010000277 DAOSYS010000000
VERSION     DAOSYS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15581_length_4565_cov_3.244346"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..3610)
                     /locus_tag="PLU99_13755"
     CDS             complement(233..3610)
                     /locus_tag="PLU99_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOO18155.1"
                     /translation="MSERKEEPSLAPQHPALSPTRPLFIDNRDGNTLDRAIADHLRTL
                     RQEMAVPWEVCMATAFFNVQGFNLLADELEQVAHVRLLLGAEPTPEAVTPLRRPGDPP
                     EPKFTRCQVSAALKNLDAGLARDRDLLPFDDATDKALRRLLTVLHPTRDGERPKLEVR
                     RFENHFLHAKAFVFRVRGGGLLVGSSNFTAGGLRRNLELNLGHYEDPVVGKVETWFDE
                     LWEQAAPYDLAAIFDRLMAEYPPYLIYLRVLLALYGTELEEEADEIAGPGNIPVTTFQ
                     KHGVWRARRILRRFHGVLIADGVGLGKTFIAGEIIRLYQLERRPVLLVCPAALRDSTW
                     DDFLRDYDFQRSVECVSYEQLAGDEQLGGNQRHLRRPVDEFALVVVDEAHNYRNPEAP
                     ARAAVLRRLLMGQRRDLVFMSATPVNNSLWDLYHVLRYFVKQDAALADRGVLSIRERF
                     ESAMREDPFNLNPDLLYPIIDATTVKRTRQFIKKYYGNDLIRLRDGSRVPIRFPKPIA
                     SSINYDLEEVLPGFLGDVEQALMPPDGQPCLTMARYQPENYPVGEVRAEADSALVGLI
                     RSGLLKRFESSVHAFAQTTAKMVEEHDLFLRGLDQGIIIRKELMHELSAADDDEVIDE
                     LLMTSSQAETAVNYNVPELRAAVQADRDVLKRLCDKARKVKPDLDPKLAALVEELARI
                     AADAKKEAIDDQDQRRKQKVLVFSFYEDTINCIESFLSRAIEKDKRLVCYRGRMASVA
                     GSDARGGVTREAAVYGFAPESSGAPPARREDRFDLLLCTDVLAEGMNLQQCRNIINFD
                     MPWNPMRLVQRHGRIDRIGSKHHRVFLRTFFPDRQLDALLNLEQRVRSKLAQAAASVG
                     VEVAPIELGAEGHQSFSETREEIERLHRGDASIYEAGGTQSAAQTGEEYRQELRKALQ
                     RQRDEIEGLPWKAGSGMAKGKHRGHFFCATVGDRVYLRFVPYGDGPFISELGTCLRIV
                     ECREDTPRVMPQDLKQTAYAAWERARQHIFASWTHETDPANLHPRVPRLNHEIAEFLR
                     AHPPADADQTRLEACLDAIEAPCPRREQNLLRDVFVANYPSHAAKSKAIMDQVERIGL
                     QPFAAPAPLPPIQPDEVHLICWMAVEEATPGRDRLTP"
     gene            complement(3711..4133)
                     /locus_tag="PLU99_13760"
     CDS             complement(3711..4133)
                     /locus_tag="PLU99_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOO18156.1"
                     /translation="MNTYFADTFFYRALFDHRDEHHDRVRTWIQGKRFRTVTTQFVLT
                     EVADEFRRTPLRTVFLALLKELRQNALVEIIPATPGLVDQGIEHYRKYRDKEWGLTDC
                     ISFVVMATRGVHEALTADKHFEHAGFQAVLKPKGNNRT"
     gene            complement(4130..>4565)
                     /locus_tag="PLU99_13765"
     CDS             complement(4130..>4565)
                     /locus_tag="PLU99_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018504417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18157.1"
                     /translation="PSRGDLLWLRAVQLAGRLAAVDDRYADWAAAVGVECGPVDAKLK
                     FDMICELDAVVALLYGLDEKHLRHIFETFHEGWGPGQTATHPTLGEYDVRLKTTLADY
                     RAWTKKAYPTAYQSLKPLIEMAREIQAEDADAGDAPKRSRLR"
BASE COUNT          843 a         1376 c         1387 g          959 t
ORIGIN      
        1 aacctacgcg atcaggtgcg tgttcctcaa aatggctgct tttgaagtga taatcggtgg
       61 cggggtttaa gtgataactg acacgtggtt gcgcgccaat ggcggggagg ggctgcttgg
      121 ggtgataaag tgatagggtg acaaagaaag ctttgagctt tcgaccatca gctttgagcc
      181 agaatgcgtg gattggccca ggagccgtgc gctcatttgc ctgcgcggta cctcaaggcg
      241 ttaatcggtc tcgccccggc gtcgcctcct ccaccgccat ccagcaaatc agatgcactt
      301 cgtccggttg aatgggtgga agcggggcgg gcgctgcgaa gggctgaagg ccgatccgtt
      361 caacctgatc catgattgct ttcgatttcg ccgcgtggct cgggtaattt gccacgaaaa
      421 cgtcccgaag cagattctgc tcgcgtcgcg ggcatggagc ttcgatagcg tccaagcagg
      481 cttcgaggcg ggtttgatcg gcgtcggcgg gcgggtgcgc gcggaggaac tcagcgattt
      541 cgtggttcag ccggggcaca cgcgggtgca ggttggccgg atccgtctcg tgagtccacg
      601 acgcgaagat gtgctggcgc gcccgctccc aagcggcgta tgcggtctgt ttcaggtcct
      661 gcggcatgac ccgcggcgtg tcttcacggc attctacgat gcgtaggcat gtaccgagtt
      721 cgctaatgaa cggcccgtcg ccgtacggca cgaagcgcaa atatacgcgg tcgcctacgg
      781 tggcacagaa aaagtgtccg cggtgtttac ctttggccat cccagagcct gccttccacg
      841 gcagaccctc aatttcatcg cgttgccgtt ggagagcttt tcgcagttcc tgacggtact
      901 cctcgccggt ctgggcggcc gattgcgttc cccccgcctc gtaaatgctg gcgtcgccgc
      961 gatgcaggcg ctcgatctct tcgcgtgttt cggaaaaaga ttggtgtcct tcagcgccta
     1021 gctcaatagg tgcgacttcc acgccgacgg aagcggccgc ctgcgccagc tttgagcgca
     1081 cccgctgctc gagattgagc aatgcatcaa gctgccggtc cgggaagaac gtgcgcagga
     1141 agacgcggtg gtgcttgctg ccgatgcggt cgattcggcc atgccgctgt accagccgca
     1201 tcggattcca gggcatatcg aagttgatga tgttgcggca ctgttgcagg ttcatgcctt
     1261 cggccagcac atcggtacag agcagcaaat cgaagcggtc ctcgcgcctc gccggcggcg
     1321 cgccggacga ttcgggtgcg aagccgtaga ccgccgcttc gcgggtaacg ccgccgcgcg
     1381 cgtcactgcc ggccacggat gccatgcgcc cgcggtagca caccaggcgc ttgtccttct
     1441 cgatcgcccg gctcaggaac gactcaatgc aattgatggt gtcctcataa aacgaaaaca
     1501 cgaggacctt ctgctttcgt cgctgatcct ggtcgtcgat tgcctctttc tttgcatccg
     1561 cggcgatacg ggccaactct tcgaccagcg cggcgagctt cgggtcaagg tcgggtttca
     1621 ctttgcgtgc cttgtcgcac aggcgcttca gtacatcacg gtccgcctgc accgcggccc
     1681 gcagttccgg aacgttgtag ttgacggccg tttcggcctg cgagcttgtc atcagcagtt
     1741 cgtcgattac ttcatcgtcg tctgcggccg aaagctcgtg catcagctcc ttgcgaatga
     1801 ttatgccctg gtccaggcca cgcaagaaca ggtcatgttc ttcgaccatt ttcgcggtgg
     1861 tctgcgcgaa ggcgtgtacc gatgattcga agcgcttcag gaggcccgac cgaatcaggc
     1921 cgaccaaggc tgaatcggct tcggctcgca cctcgcccac tgggtagttt tccggctggt
     1981 agcgggccat cgtcaggcac ggctgtccat cgggcggcat cagcgcctgc tcaacgtcgc
     2041 ccaggaaacc gggcaaaacc tcctccaggt cgtagttgat cgaagacgca atgggtttag
     2101 gaaagcggat cggcacgcgg gagccgtcac ggagtcggat cagatcgttt ccgtagtact
     2161 tcttaataaa ctgcctcgtg cgcttgaccg tggttgcatc gatgatcggg tatagcaggt
     2221 cgggattcag gttgaagggg tcctcccgca tcgcgctctc aaaacgctca cgtatcgaca
     2281 gcaccccgcg gtccgccaat gccgcatcct gtttcacgaa ataacgcaaa acatggtaga
     2341 ggtcccacag cgaattatta accggcgtcg ctgacatgaa cacgaggtca cggcgttgtc
     2401 ccatcagcag tcgccggagt acggcagcgc gcgcgggcgc ctccgggttt cggtaattat
     2461 gcgcctcatc cacgacgacg agcgcgaact catcaacagg gcggcgcagg tgccgctgat
     2521 taccgccgag ctgctcatcg cccgccagtt gttcgtatga aacgcactca actgagcgtt
     2581 gaaagtcata gtctcgcaag aagtcgtccc acgtactatc gcgcaacgcc gcgggacata
     2641 cgagcagcac cggtcgccgc tcaagctgat acaagcggat gatttctccg gcgatgaagg
     2701 tcttgcccag cccgacgcca tccgcgatca gcacgccgtg gaacctgcgc aaaatgcgcc
     2761 gcgcccgcca gacgccgtgc ttctgaaaag tggtaaccgg aatattgccc ggcccggcga
     2821 tttcgtcggc ttcctcttcc aactctgtgc cgtacagcgc cagcaggacg cgcaggtaga
     2881 tcaggtaagg cgggtactcg gccattagcc ggtcgaagat cgccgccagg tcgtacgggg
     2941 cggcctgctc ccatagctca tcgaaccatg tctcgacctt gcccaccacc gggtcttcgt
     3001 aatgcccaag gttcagttcc aagtttcggc gcagaccgcc ggcggtgaaa tttgacgaac
     3061 cgaccagtag gccgccgccc ctgactcgga agacgaacgc ttttgcgtgg aggaagtggt
     3121 tttcgaaccg ccgcacttcc agcttcggcc gctcaccgtc gcgcgtcggg tgtagtaccg
     3181 tcagtaagcg ccgtaacgcc ttgtctgtcg cgtcgtcaaa cggcagcagg tcgcggtcac
     3241 gggccaaacc ggcatccaag tttttcagcg ccgcgctgac ctgacaccgc gtgaacttcg
     3301 gttctggcgg atcgcccggc cgacgcagtg gcgtgacagc ttccggcgtc ggctccgcgc
     3361 cgagcagtag gcgaacgtga gcgacctgtt ccagctcgtc ggccagcagg ttgaatccct
     3421 gtacattgaa gaatgccgtg gccatgcaga cttcccaagg cacggccatt tcctgccgca
     3481 acgtgcgcag gtggtcagcg attgcccggt caagcgtgtt gccgtcgcgg ttgtcgataa
     3541 acagcgggcg agtcggtgaa agtgccgggt gttgcggggc cagggagggt tcttctttcc
     3601 gttcgctcac gtggcggctc cgtgtgaatg gttggacctg cccgggtcat tcagactgag
     3661 tttgatccct ttgtggcttc ggtgcattac gaggtccgtc ttgcgctcgt ttatgtcctg
     3721 ttattccctt tgggcttaag cacggcctga aagccagcgt gttcaaagtg cttgtcggcg
     3781 gtcagcgctt cgtgcacgcc gcgagtagcc atgactacaa acgaaatgca atcagtgagg
     3841 ccccattcct tgtcccggta cttacgataa tgttcaatgc cctggtctac caaacccggg
     3901 gtagccggga tgatttccac cagtgcattt tgccgcaatt ccttcaatag cgcgagaaac
     3961 acggttctta acggtgtcct gcggaactca tcggcgactt cggtcaggac aaactgggtt
     4021 gtaactgtgc ggaagcgctt tccttgaatc cacgtcctca cccggtcatg atgttcgtcg
     4081 cgatggtcga acaacgcgcg gtagaaaaaa gtgtcggcga agtatgtatt catcgcaggc
     4141 ggctccgctt cggggcatcg cccgcgtctg cgtcctcggc ctggatttca cgcgccatct
     4201 cgatgagggg ttttaaagac tggtatgccg tcggatatgc cttcttcgtc caggcccggt
     4261 agtcagcgag cgtggttttc aggcggacgt catactcgcc gagcgtcgga tgcgtggcgg
     4321 tctggccggg accccagcct tcgtggaagg tctcgaagat gtgccgcaga tgcttttcat
     4381 ccaggccgta tagcagcgcc actaccgcgt ccagctcgca aatcatatcg aacttgagct
     4441 tggcatcaac ggggccgcat tcgacgccga ccgcggcggc ccagtcggca tagcggtcat
     4501 ccaccgcggc cagccggccc gccaattgaa ccgcgcgtag ccacagcaga tcgccacgcg
     4561 acggg
//
LOCUS       DAOSYS010000278         4499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15833_length_4499_cov_5.558281, whole genome shotgun sequence.
ACCESSION   DAOSYS010000278 DAOSYS010000000
VERSION     DAOSYS010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15833_length_4499_cov_5.558281"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..860)
                     /locus_tag="PLU99_13770"
     CDS             complement(123..860)
                     /locus_tag="PLU99_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18158.1"
                     /translation="MSEAARACATITLAYHGLEFRCPSAGVSEDRPLAPEDVALLESG
                     AKQYQQMARQDTADELLKLGEEMFTWLNGPTRTLSRMLDTCQPPLLIEFEALAKTENG
                     EPAEEAKRARAFLNAPWELLAADGLHWALRDGILFCPVRRVGKPLSPPAASLHRLSLV
                     FMAAAPRGADNLDYEAEEASILTATQNLRLNMVVEESGPLDLLAACSVSSQARNPTAR
                     SATPPSRWRPGTPRWGCETGTQLVSEV"
     gene            1169..1495
                     /locus_tag="PLU99_13775"
     CDS             1169..1495
                     /locus_tag="PLU99_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18159.1"
                     /translation="MVRQSVFPRLVVRRKSHAGSRLLSASEWREAAQFFRLSRIELGI
                     VKGIFDELTAAQMGRKLGLAERTVRAYARRVRVKLRAERKHIVVLRVFRWFTSRDRRT
                     EPPLTA"
     gene            1601..2500
                     /locus_tag="PLU99_13780"
     CDS             1601..2500
                     /locus_tag="PLU99_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO18160.1"
                     /translation="MFKPLLVIPPAPARWPALRELPIHPDPLWQDDLEKRFAQGLPRS
                     QDAFAIIPDGGLVLGCAALSKRHDLGVLSRLFVQPEHRGRGYARRLLETLIAWFDMTG
                     GKWLYATATADLAEGLLRKVGFKTIRRAPRTPHDAVVVMRLTGEIYDDPLATADGRLT
                     IHDVSRANWPTMVVLLHNRPGPDPRISLDESAVGADQAALELIAQQEAGKCLLKAAFR
                     GTRLIALGTLAIDQPGERTYAMITPHSDAPPALREALLEVARGKGYSQVDFPMEALAA
                     PEVVVPITPGPAPEAGETGRGRQ"
     gene            complement(2570..3577)
                     /locus_tag="PLU99_13785"
     CDS             complement(2570..3577)
                     /locus_tag="PLU99_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOO18161.1"
                     /translation="MTVSARPCSPPAAASARSAPGLLVTFRLLLYGGLLATAVWGSPL
                     VRVAALCAFPLALFVDWEGGVRHLLRLGGIALAAWLAPQYGSRLGDLISTHWGLSSTV
                     SNLAGIAIIAILIVAAGAILARFASRHVRRTRFTGACDHMLGGILGSAEASVAVVTVC
                     WLLAAFSGPIATLRARTQQQQLATSQPSQQRILNTLSNLAAAVRGDPTGQWLLQRNPL
                     EKSAPVQTAGLMLELTADPGALLSAADQGRLDAIAALPEVRKYIEAIERDPQLREALQ
                     NQDIVAVLSHPLVKSMLADAELRRALLENLEQLRKALGLEHEALGLDDEALGSSVGID
                     Q"
     gene            3871..>4499
                     /locus_tag="PLU99_13790"
     CDS             3871..>4499
                     /locus_tag="PLU99_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOO18162.1"
                     /translation="MNLYVGTSGYSYPQWKGTFYPRDLPAKQMLRYYGEHFRAVEINN
                     TFYRMPKASVLEGWAEQVPADFQFVLKAPQRITHMQRLKEADESVSYLLDVAAVLRER
                     LGPLLFQLPPNLKKDVPRLRAFLALLPGRRAAFEFRHQSWFDDEVFDLMRQHGAALCV
                     AEAENDLAVPLVSTADWGYLRLRRPDYGKADLRRWAEQVARQNWGDLFV"
BASE COUNT          788 a         1300 c         1592 g          819 t
ORIGIN      
        1 ttgagcacgt gataaacgaa accaccgggt gctgcacgtg cttgtcttgc catgcggtca
       61 acttagccga tcgcgcagca cgcgccaaga aacagtgcta agaaactagc tgcgtccccg
      121 tttcacacct cagaaactag ctgcgtcccc gtttcacacc cccatcgggg cgttccaggc
      181 cgccagcggg aaggcggcgt cgccgagcgt gccgtcggat tgcgcgcctg gctggagacc
      241 gaacaggcgg cgagtaggtc aagggggccg ctttcctcga cgaccatatt gaggcgcagg
      301 ttctgcgtgg cagtgaggat cgaggcttcc tcggcctcat agtcgaggtt gtcggcgccg
      361 cgcggggccg cggccatgaa gaccaggctc aagcggtgga ggctggcggc gggtggcgaa
      421 agcggtttac cgacgcgccg gacggggcag aaaagaatgc cgtcacgtag ggcccaatgc
      481 agaccgtcgg cggcgaggag ctcccagggg gcgttaagga acgcgcgagc acgcttggct
      541 tcttcggcgg gttcgccgtt ctcggttttg gccaatgctt cgaactcgat cagcagcggc
      601 ggctggcaag tgtccagcat gcggctgaga gtgcgcgtgg ggccattgag ccaggtgaac
      661 atttcttcgc cgagcttgag cagttcgtcg gccgtgtcct gacgcgccat ttgctggtat
      721 tgcttcgccc cgctttcaag cagcgcgaca tcttcgggcg caagcgggcg atcctcgctc
      781 acaccggccg acgggcagcg gaattcgagg ccgtgatagg cgagcgtgat tgtggcgcac
      841 gccctggctg cttctgacat ccgcaatact ccgggtttga ggacggttgt tcggactggc
      901 acagtcctca atagcctgag gcggaacgga atgcaagagt tggtgggcct caatggccgc
      961 aaatgagaca ctgcttgtcg tcaacagaaa cgccgcgtgt gtagtgagag tggggccgta
     1021 gggaaaaatt ccagaaaatt tttccatttt cgtgtaagaa tcgggcgcag tttacgtttt
     1081 atacccggga gcgcgggacg ccggtttacg gggcacgatt cggcggcctt cggggtgacg
     1141 gtgcgggcca ggtgaggagg aaagcatcat ggtcaggcag tccgtgttcc cgcggctggt
     1201 ggtgcgccgc aagtctcacg ccggcagcag gttactgagc gcgagcgagt ggcgtgaagc
     1261 ggcgcagttc tttcggctgt cgcggattga gctggggatc gtcaaaggca ttttcgacga
     1321 gttgacggcc gcacaaatgg ggcggaagct cgggctggcg gagcgtacag tccgcgcgta
     1381 cgcgcggcgc gtgcgcgtta agctgcgagc ggagcgcaag catatagtcg ttctgagagt
     1441 cttcaggtgg tttaccagcc gcgaccggcg aactgagccg cccctgacag cttagaagcc
     1501 tgtcccgcaa caaatcgcag gcggcttggc ccggcgcggc cgctgcggta gcatggccgc
     1561 tcggttttgt taggtctcgt ggctgcaaag agttaggtgc atgtttaaac cgttgctcgt
     1621 cattccgccc gcgccggctc gctggccggc gctgcgcgag ttgccgattc accccgaccc
     1681 gctgtggcag gacgacctgg agaaacgctt tgcgcaaggc ctgccgcgca gtcaggatgc
     1741 gttcgccatt attccggacg gcgggctcgt gctcggctgt gccgcgctca gtaagcggca
     1801 tgacctgggc gtgttgagcc ggctgttcgt gcagccggag catcgcgggc gtgggtatgc
     1861 gcggcggctg ctggagacgc tgatcgcatg gttcgacatg accggcggga agtggctgta
     1921 tgccacggcg acggcggatt tggcggaggg attgctgcgg aaggtggggt tcaagacgat
     1981 ccggcgagcg ccgcgtacgc cgcacgacgc cgtggtcgtg atgcgactga cgggcgagat
     2041 ttacgatgac ccgctggcca cggcggacgg gcgattgacg attcacgacg tgtcccgggc
     2101 caactggccg acgatggtgg tgctgctgca caatcgcccc gggccggatc cgcggatttc
     2161 gctggatgag tccgcggtcg gggcggatca ggcggccctg gagctgattg cccagcagga
     2221 agccggcaag tgcctgctca aagcggcgtt tcgcggaaca cggctgattg cgctggggac
     2281 gctggcgatc gatcagcctg gcgagcggac gtacgcgatg atcacgccgc attcggacgc
     2341 gccgccggcg ttgcgcgagg cgctgctgga agtggcgcgc ggcaagggat actcgcaggt
     2401 ggactttccc atggaggcgc tggccgcacc ggaagtggtc gtgccgatca cgcccgggcc
     2461 tgcgcctgag gcgggcgaga ccgggcgggg ccggcagtaa taacgccgcc gccggacttg
     2521 ggcattcgag cagactttgc acgcgccgtg ggcttggctg gcgctggctc tattgatcaa
     2581 tgccaacgct ggagccgagg gcctcgtcgt ccaggccgag agcctcgtgt tccaggccga
     2641 gcgccttccg taactgctcc aggttctcca agagtgcgcg gcgcagttcg gcgtcggcga
     2701 gcattgactt aaccagcgga tgacttagca cggccacaat gtcctggttt tgcagggcct
     2761 cgcgcaattg cgggtcgcgc tcgatggctt cgatatactt gcggacctcc ggcagggcgg
     2821 cgatggcgtc aaggcggccc tggtcggcgg ccgacaacag ggccccgggg tcggcggtca
     2881 actccagcat gaggccggca gtctgcaccg gcgctgattt ttccagcgga ttgcgttgga
     2941 gcagccattg cccggtgggg tcgccgcgca ccgccgcggc cagattcgag agcgtgttga
     3001 ggatgcgctg ctgcgatggc tgcgaagtcg cgagctgctg ctgttgcgtg cgagcccgca
     3061 gcgtggctat tggtccagaa aacgccgcca gaagccagca tacggtcacc actgccacgc
     3121 tggcttcggc cgagccaagt atgccgccga gcatgtggtc gcaggcgccg gtgaagcggg
     3181 tgcggcgcac gtggcgtgac gcgaagcgag ccaggattgc acccgccgcg acaattagga
     3241 tcgcgattat cgcaatgccg gcgagattgc tgacggtcga agacaggccc cagtgcgtgc
     3301 ttattaagtc gccgaggcga ctgccgtact gcggggcgag ccaggccgcc agcgcaatgc
     3361 cgcccaaccg cagcaggtgg cgaacgccgc cttcccaatc cacgaacagc gccagcggaa
     3421 aggcgcagag ggcggccacg cgcaccaacg ggctgcccca gacggcagtc gccaagaggc
     3481 cgccatagag cagcagcctg aacgtcacca gcaggccggg cgcggatcgg gcgctggcgg
     3541 cagcgggggg cgaacacggg cgggcgctaa cggtcatgtc ctcacatcca cattattcat
     3601 aacgcgactg atttttcata acgcgactac tgccgtccat tcgtccgata gtaccgcgac
     3661 gaagtccggc ggcacagtct gcaagggaat tcgcggatgc acatcgcata ttttgcgctt
     3721 ccggcggcct gagggaaatg actgggctga taaccggcaa agcaagacgg cccgccgcgc
     3781 ggcctcgtcg cgtcataaca tcaggttgcg aatgtcgaaa cggggccgct tatccgatcc
     3841 agccgccgct tcagccgcgg aggaatactt atgaatctgt acgtcgggac gagcggctat
     3901 tcctacccgc agtggaaagg cacgttttac ccgcgggatt tgccggccaa gcagatgctc
     3961 cgatactacg gtgagcattt ccgggcggtg gaaatcaaca atacgtttta ccgcatgccc
     4021 aaagcgtccg tgctggaggg ctgggccgag caggttccgg ccgatttcca atttgtgctg
     4081 aaggcgccgc aacgcatcac gcatatgcag cggctcaagg aggccgacga atcggtttcg
     4141 tatctgctgg acgtggccgc agtgctgcga gagcgtttgg ggccgctgct gtttcaattg
     4201 ccgccaaatc tcaagaagga tgtgccgcgg ttgcgcgcgt tcctggcgct attgccgggg
     4261 cgccgggcgg cgtttgagtt tcggcatcag tcgtggttcg atgatgaggt ttttgatttg
     4321 atgcgccagc acggggcggc gctgtgcgtt gcggaggcgg agaacgactt ggcggttccg
     4381 ctcgtgtcga cggctgattg gggctacttg cggctgcggc ggccggacta tggcaaggcg
     4441 gatttgcgaa ggtgggcgga gcaagtggcg cggcagaact ggggcgacct gtttgtgtt
//
LOCUS       DAOSYS010000279         4484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15899_length_4484_cov_4.325581, whole genome shotgun sequence.
ACCESSION   DAOSYS010000279 DAOSYS010000000
VERSION     DAOSYS010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15899_length_4484_cov_4.325581"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLU99_13795"
     CDS             complement(<1..754)
                     /locus_tag="PLU99_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOO18163.1"
                     /translation="MAKQRVVIVGGGLAGLSAAMQLAEEEVGVDLISMVPVKRSHSVC
                     AQGGINCVNKFTRAQGDTEWNHFDDSVYGGEFLNNQPPVKQMCEWAPHIVDLADRLGV
                     PFNRSDEGNLATRRFGGTLYKRTIYAGASTGQQLLYALDEQVRRWEVEGRVKKYEFWE
                     FLGPILDEQGICRGCVAQDMFSLEIKAFPADAVIMATGGPGLVYGKSTMSMVCTGAAN
                     ARAYKAGAIYANGEFVQVHPSAIPGADKLRLIS"
     gene            1031..1654
                     /locus_tag="PLU99_13800"
     CDS             1031..1654
                     /locus_tag="PLU99_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOO18164.1"
                     /translation="MFSIIPRNVAFFDLFDKAGQIIVKAAETYAQLGRDYEHRDDHLA
                     RIRQLEHDGDDIAHRTLDRLDQSFITPFDREDIQTLMVRLDDVVDEIDAGAKRLAIYK
                     ITELTPAFIKQTDVLLKACQLTAQAVMRLRDIRKPDGLHEKLVEIHYLENIGDQNNHA
                     ALAELFETVKDPLLVMKWKEIYDLIERAIDRCEDIANIIRGIILKHA"
     gene            1663..2685
                     /locus_tag="PLU99_13805"
     CDS             1663..2685
                     /locus_tag="PLU99_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOO18165.1"
                     /translation="MLEIYVVIIILIALVFDFCNGLNDAANSVATVVTTRVLSPRVAV
                     LWAAFFNFVAAFGFQMHVASTIGRDLIHPSVVNPHLIFATLISAVAWTFICTKRGLPI
                     SVSHALVGAMAGAALVGGGWSALVWWQIGKIAAFIVLAPLIGMLLGAANMVASMWICR
                     RMAPRTVDGVFRVLQLVSAAVYSLSHGLNDAQKTMGIIFALLISVPSLQSWATHDGDP
                     NSQQLAWWIILSCQTAMALGTYFGGWKIVHTLGHRVTKLRPIDGFGAETGGGVTILAL
                     SQLGIPVSTTHTITGAIFGVGAVRRMTAVRWSVGQSIVIAWVLTLPASAALSAALYYV
                     LRLFVA"
     assembly_gap    2872..2971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2972..4471
                     /locus_tag="PLU99_13810"
     CDS             <2972..4471
                     /locus_tag="PLU99_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18166.1"
                     /translation="NDFVIYPISVEQNLMHGVVLGLLVASIVAVPISVAILYRFAYAL
                     PFIAAVLVFAHMPWMALTLVGSCILAAVKPFRLSFRFGAALVGMLPVIVYLYLAMQGA
                     PQQLGTYSSPDQKLLLSAPWLLAILAACAMLGIIMAFSRIANYRRGAVAPVMAIMFAT
                     PAILFARYVGFDEVSYRVLESVYGPRAPMFATRDVSGDIFSLIEERLESADRERMLSW
                     FLSDANKRADLLHRLVGVLQRAFLADRREASEACRRFIADHPSSRYVPCALYIRAHAL
                     DTRLDLRKLLAPDIALEREPYSDFPHVESEEVWQALLTNYPESHLSAAARLRLAQLRL
                     RRGDVAAAADLLRPAIEADAPESRPATRPSGPLSRSARPESSLEVDPGPYLAEARHLY
                     ELIAANRDDPRYGNEPLRRLAGLDPHRAKYRAELLKLAAQFPQALLEDNIMAEWAAAT
                     PDRAEREANLAACIAAYPDGDGAKRAKRDLLRLRDLQKRPESNTPAVTP"
BASE COUNT          764 a         1460 c         1336 g          824 t
ORIGIN      
        1 cggagatcag ccgcagctta tccgcacccg gaatcgccga cggatgcacc tgcacgaact
       61 cgccgttggc gtagattgcg ccggccttgt acgcccgcgc gttcgccgcc cccgtgcaga
      121 ccatcgacat cgtcgacttg ccgtagacca ggcccggtcc gccggtcgcc atgattaccg
      181 cgtcggccgg aaacgccttg atctccagcg agaacatgtc ctgcgccacg cagccgcggc
      241 agatgccctg ttcgtccagg atgggcccga ggaactccca aaactcgtac ttcttgacgc
      301 ggccctccac ctcccaccgc cgcacctgct cgtccagcgc gtaaagaagc tgttgtcccg
      361 tggatgcgcc ggcgtagatc gtgcgcttat acagcgtgcc gccgaagcgc cgcgtggcca
      421 ggttgccctc atccgagcgg ttaaacggca cgcccagccg gtcagccaaa tccacgatgt
      481 gcggcgccca ctcgcacatt tgcttgacag gcggctggtt gttcaggaac tccccgccgt
      541 aaacgctgtc atcgaagtgg ttccactcgg tgtcgccctg ggcgcgagtg aacttgttga
      601 cgcaattgat gccaccttgg gcgcacaccg aatggctgcg cttaaccggc accatgctga
      661 tcaggtcgac cccgacttct tcctcggcaa gctgcatggc cgctgacagc cccgccagcc
      721 caccaccgac gattacgacg cgttgtttag ccatgcgttg cagcctttct ggccgcccag
      781 atgcggaaac actgaatgat tgaccgcgct acgctaaatt atgtggggcc ggccgcaacc
      841 gatcaaccgc gaccggcggc gagcgttgga gtaagacggt gcagcgccac cggtattacg
      901 atggaccgcc agcgggatta cacgcggccc gcgggttctt gcaagctcgc cttggcatct
      961 cgcccctgcc gctctaaaat gctgacactt tgcggtgggg tactagtggt cggcgcgcag
     1021 aggtgactca ttgttcagca tcatcccccg aaacgtcgcg ttttttgacc ttttcgacaa
     1081 ggccgggcag atcatcgtta aagccgcgga aacttacgcc caactgggcc gcgattacga
     1141 gcaccgggac gaccacctgg cccgcattcg ccagctcgag cacgacggcg acgacatcgc
     1201 tcaccgcacc ttggatcgcc tggatcagag cttcatcacg ccttttgacc gtgaggacat
     1261 tcagaccctg atggtccgcc tcgacgacgt ggtggacgag atcgacgccg gcgccaaaag
     1321 actcgcgatc tacaagatca ccgagctcac cccagccttc atcaagcaga cggacgtgct
     1381 cctcaaagcc tgccagctca cggctcaggc cgtaatgcgg ctgcgcgata ttcgcaagcc
     1441 tgacggcctg cacgagaagc tggtcgagat ccactacctg gaaaacatcg gtgaccaaaa
     1501 caaccacgcg gccttggccg agctgttcga gactgttaaa gacccgctgc tggtcatgaa
     1561 gtggaaggaa atctacgacc tgatcgagcg ggcgatcgac cgctgtgagg acatcgccaa
     1621 catcatccgc gggatcattc tgaaacacgc gtagctgggc caatgctcga aatctacgta
     1681 gtaatcatca ttctgatcgc actggtcttt gacttctgca acgggctgaa cgacgccgcc
     1741 aactccgtcg ccacggtcgt caccacgcgc gtgctcagcc cccgcgtggc ggtgctttgg
     1801 gcggcgttct tcaactttgt ggccgccttc ggcttccaaa tgcacgtggc ctccaccatc
     1861 ggccgcgatc tgatacaccc atcagtggtg aacccgcacc tgatctttgc cacgttaatc
     1921 tccgccgtcg cctggacgtt catctgcacc aagcgcggcc tgccgattag cgtttcgcac
     1981 gccctggtcg gcgcgatggc cggggcggcg ctggtcggcg gcggttggag cgcgttggtt
     2041 tggtggcaga tcggcaagat cgccgcattc atcgtgctgg cgccgctgat tgggatgttg
     2101 ctcggggcgg cgaacatggt agcgagcatg tggatctgcc gccgcatggc cccgcgcacc
     2161 gtcgatggcg tcttccgcgt tctgcagctt gtgtcggccg cggtttacag cctcagccac
     2221 gggttaaacg acgcccagaa gaccatgggc atcattttcg cactgctcat cagcgtgccg
     2281 tcgctgcaaa gctgggcgac gcacgacggc gatccgaact cgcagcaact ggcctggtgg
     2341 attattttga gctgtcagac cgccatggcc ctcggcacct acttcggcgg ctggaaaatc
     2401 gtgcatacct tgggccatcg cgtaaccaag ctgcggccca tcgatggctt cggggccgag
     2461 accggcggcg gcgtgacgat tctggcgctc tcgcaactgg gaattcccgt cagcaccacg
     2521 cacacgatta ccggcgcgat tttcggcgtc ggcgccgtgc gccgcatgac cgcggtccgt
     2581 tggagcgtcg gccagagtat cgtgattgcc tgggtgctga ccttgccggc ctcggccgca
     2641 ctctcggccg ccctgtacta cgtgctgcga ttgttcgtcg cataggtccc cggggcggca
     2701 cggcccggct cagccattgc gtcaatcgca atccaaagcg gtcagaacaa aatgcccgaa
     2761 aaggagcggc aagacggtgc gtcggccctg attgcatccc cgccgcggcg ggttagcatg
     2821 gcgggcatgg cgcagccatc cagtccgagt gcagccaccg gcgagcccgc gnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naacgacttc gtaatttacc ccatcagcgt
     3001 cgagcagaac ctgatgcacg gcgtggtgct cgggctgctg gtggcttcaa tcgtggccgt
     3061 accgatttcg gtcgccatcc tctaccgttt cgcgtacgcg ctgccgttta tcgccgcggt
     3121 gctcgttttc gcgcacatgc cctggatggc gctgacgctg gtgggctcgt gcatcctggc
     3181 ggccgtcaag ccgttccgcc tctcgttccg cttcggggcc gccctggtgg gcatgctgcc
     3241 ggtaatcgtt tacctgtact tggctatgca aggcgcgccg cagcagctcg gcacgtactc
     3301 ctcgcctgac cagaagctgc tgctgtcggc tccctggctg ctggccattt tggcggcttg
     3361 cgcgatgctg ggaatcatca tggcgttttc gcgcatcgcg aattatcggc ggggcgcggt
     3421 ggcaccggtg atggcgatca tgttcgccac gcccgccatt ctgtttgcgc gctacgtcgg
     3481 ctttgacgaa gtgagctacc gcgtgctgga gtcggtgtat gggcctcgcg cgcccatgtt
     3541 tgccacccgc gacgtcagcg gcgacatctt cagcctgatc gaggaacgct tggaaagcgc
     3601 cgatcgcgag cgcatgttga gttggttcct gtcggatgcg aacaaacgcg ccgacctgtt
     3661 gcaccggctc gtgggcgtct tgcagcgcgc cttcctggcg gaccgccgtg aggcctcgga
     3721 agcctgccgc cggttcatcg ccgatcaccc cagcagccgg tacgtgccct gcgccttgta
     3781 catccgggcg cacgcactcg acacgcgatt ggacctgcgg aagctgctcg cgccggatat
     3841 tgcgctggag cgcgagccgt actccgactt cccgcacgtc gagtcagagg aagtctggca
     3901 ggcgctgctc accaattatc ccgagtcgca tttgtcggcg gcggcgcgcc tgcgcttggc
     3961 acaactgcga ttgcggcgcg gtgacgtggc ggccgcggcc gacctgctga ggccggctat
     4021 cgaagccgac gcgccagaaa gccggcccgc tacgcggccg agcggcccgc tgtcgcgcag
     4081 cgcccgcccg gaaagcagct tggaagtaga ccctggccca tacctggcag aggcccggca
     4141 cttatacgag ctgattgccg ccaaccgcga tgacccgcgc tatggaaacg agccgctcag
     4201 acgcctggcg gggctggacc cgcatcgcgc caaatatcgc gccgagttgc tgaagctcgc
     4261 cgcccagttt ccccaggcct tactcgaaga caacataatg gcggaatggg cggccgccac
     4321 ccccgaccgc gcggagcgtg aagccaatct cgcggcctgc atcgccgcct acccagacgg
     4381 cgacggggcc aagcgggcca agcgtgacct tctgcggctg cgcgatctgc aaaagcggcc
     4441 tgaatccaac acgccagcgg tgacgccgtg aaacaagtgg tatt
//
LOCUS       DAOSYS010000280         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15937_length_4471_cov_4.768569, whole genome shotgun sequence.
ACCESSION   DAOSYS010000280 DAOSYS010000000
VERSION     DAOSYS010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15937_length_4471_cov_4.768569"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /gene="otsB"
                     /locus_tag="PLU99_13815"
     CDS             complement(<1..1850)
                     /gene="otsB"
                     /locus_tag="PLU99_13815"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HOO18167.1"
                     /translation="MTELQQRLDDLSRAPVLLVASDFDGTLAPIVSEPSRAEANRESL
                     VALRALAEMPQTHVAIISGRALSDLAAKTLDIERLHLVGSHGSEFEAGITTPLTPEAQ
                     ALLERIKKEVQDIAAAAPGAVVELKPAGLAFHYRNVDEQLGAATVEAILNGPGRRAGV
                     FVRSGKKVIELSVVQTDKGQALRALRQRLGASAVLFLGDDVTDEDAFATLTGPDVGIK
                     VGAGESVAPYRVAGTAEVSQLLASLSERRSEWLAGSNAVPIEQHWLLSDQRTVALLDP
                     SGSVVWYCLPRIDSPAIFASLLGGPTAGFFQVKSAEHEKPLKQRYVGDTFILETQWPG
                     FTVTDYLDCGGGRAFQRAGRTDMLRVLRGRGRVAITYAPRLDFGRVQTQLRACEGGLE
                     VEGSVDPVVLRAPGLKWDIFEEGPHQTATTLLELKDEPVVLELRYGTASLAPDPVPES
                     VRRQRTDKFWSGWAATLNLPSVAPDLVGRSALVLKALCYGPTGSFAAAATTSLPEHAG
                     GVRNWDYRFCWPRDAAMSAAALVRLGATGPALRFLDWVLGILDEMEPPALLRPVYTVT
                     GGNLGPEGEIAELPGYLGSRPVRVGNAAAQQLQLDVFGPVADLVALLAARG"
     gene            complement(1847..3319)
                     /locus_tag="PLU99_13820"
     CDS             complement(1847..3319)
                     /locus_tag="PLU99_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HOO18168.1"
                     /translation="MRLVIVSNRLPLVLEMVDGAWRTQPGAGGLIAALTPILQRQSGV
                     WIGWSGASDVDQTDELQRTLDEYQRDAGFELIPVAMTSQEVEGFYRGFSNEIIWPLFH
                     DLQSFCNFVPDYWTTYLAVNDKFAEIVAQHAQPDDLLWVQDYHLMGLGRQLRRRGLRN
                     RLAFFLHIPFPPPDIFEKLPWRVDVLEGLLKYDVLGFQTPRDRKNFADCVRDLLPDAR
                     QRRRHGGLEVRWGGQTTFVRHFPIGIDYQALAQGAALPSVATHAEEIRREMPGEQLVL
                     GLDRLDYTKGIPYRLRAFGRALERFEDLHRKVTLLQVVVPSRETVPEYQELRAEIERL
                     VTQINGGFTQPGWVPIHYVFRSLEREELLAYYCAADVALVTPLKDGMNLVAKEYCACQ
                     LDGDGVLILSEFAGAAVQLRRDALLVNPYDLDGVAEALRRAVGMSRRQRQPAMCRMQR
                     ILSRQDVHWWANQVLQACEMVKRPSGVPIEMDIASQEHPS"
     gene            complement(3525..4436)
                     /gene="ilvE"
                     /locus_tag="PLU99_13825"
     CDS             complement(3525..4436)
                     /gene="ilvE"
                     /locus_tag="PLU99_13825"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOO18169.1"
                     /translation="METATAPQNFDPDPNLKVWLNGNLITAADAKLSIFEHGLLYGDG
                     IFEGLRSYNGRVFDAAGHLRRFFDSAKAIMLDLPYQPEDIEQAFTTTLDANGLGTPDK
                     DAYLRLVATRGVGMLGMSPTRAGRPNVFVIATPLGIYRREVYEQGMRAIVASVVRNLP
                     NSMPPRIKSLNYLNNILAKIESHQAGVDEAIMLNHLGYIAEATADNVFVVRNGQLQTP
                     PTSAGILEGITRGHVIRLARETGIEVVEKNLERVDLYTADEAFLTGTAAEIVPLIEVD
                     RRKIGAGVPGSMTKELMAAYHKMVRGG"
BASE COUNT          772 a         1517 c         1397 g          785 t
ORIGIN      
        1 ccgcgcgccg ccagcaaggc caccaggtcg gccaccgggc cgaagacgtc caactgaagc
       61 tgttgtgccg cggcgttgcc gacccgcacc ggcctgcttc cgagataacc cggcagttcc
      121 gcgatctcgc cctctgggcc aaggttgccg cccgtcaccg tgtacaccgg gcgcagcaat
      181 gccggcggct ccatctcgtc gagaatcccc agcacccaat ccaggaatcg cagcgccgga
      241 ccagtggcgc ccaaacgcac caaagcggcc gccgacatgg ccgcatcgcg cggccagcag
      301 aagcgatagt cccaatttcg tacgccgcca gcgtgctccg gcaggctggt ggtggcggcg
      361 gccgcgaagg acccggttgg tccataacac agggccttga gcaccaaggc gctgcgtccg
      421 accagatcgg gcgccacgga cggtaaattc aacgtggccg cccagccact ccaaaacttg
      481 tctgtccgct gtcgccgcac ggattccggc accgggtcag gcgccaggct ggcggttcca
      541 tagcgcagct cgagcaccac cggctcatct ttcagctcga gcaacgtagt ggctgtttgg
      601 tgcggcccct cctcgaaaat atcccatttc aggccggggg cccgaagcac aaccggatcg
      661 accgaaccct caacttccag cccgccttcg caagcgcgga gctgagtttg cacgcgtcca
      721 aagtccaggc gcggtgcgta cgtaatcgcc acccgcccgc gccccctgag gacccttaac
      781 atgtccgtgc ggccggcccg ctgaaaggcc cggcccccgc cgcaatccaa gtagtcggtt
      841 accgtgaagc ccggccattg tgtttcaagg atgaaggtgt ctcccacgta gcgctgcttc
      901 agcggctttt cgtgttccgc cgatttgacc tggaagaacc ccgccgtggg tccgccgagc
      961 aggctggcga atatggccgg cgaatcgatg cgcggcaggc agtaccagac gacgctgccg
     1021 gacgggtcaa gcagggcgac cgtgcgttga tccgagagca gccaatgttg ctcgatcggg
     1081 acggcgtttg agccggccaa ccattcgcta cgccgctcgc tcagcgacgc cagcagttgc
     1141 gagacttcgg cggttccggc cacgcgatac ggggcgacgc tttcgcccgc gccgaccttg
     1201 atgccgacgt ccggaccggt cagtgtggcg aatgcgtctt cgtcggtgac gtcgtcgccg
     1261 aggaacaata ccgccgaggc gcccagccgt tgacgcaggg cgcgcagcgc ctgccccttg
     1321 tcagtctgca cgacgctcag ctcaatgacc tttttgccgg atcggacgaa tactcccgcg
     1381 cgccggcccg gcccgttgag aatggcctcc accgtggcgg cgccgagttg ctcgtcgacg
     1441 ttgcggtaat ggaaggccag cccggccggc ttgagctcca cgacggcgcc gggggctgcg
     1501 gccgcgatat cctggacctc tttcttgatc cgctcgagaa gggcctgggc ctccggcgtc
     1561 agcggagtgg tgattccggc ttcgaattcg ctgccgtggc ttccgaccag gtgcaggcgc
     1621 tcgatgtcca gggttttcgc cgcaagatcg gaaagcgccc gccccgagat gatcgcgacg
     1681 tgcgtctggg gcatctcggc cagggcccgc agcgcaacca gcgattcgcg gtttgcttcc
     1741 gcgcggctcg gctccgaaac gatgggcgcg agcgtgccgt caaagtcgct cgccacgagc
     1801 agcactggag cgcgggaaag gtcgtccaag cgctgttgca gctcagtcac gaaggatgct
     1861 cctgcgacgc tatatccatt tcaatcggca cccccgaagg gcgcttgacc atttcgcagg
     1921 cctgaagcac ctggtttgcc caccagtgca cgtcctgccg gcttaaaatc cgctgcatgc
     1981 ggcacatcgc gggttggcgc tgccggcgcg acatcccgac cgcgcgcctt aacgcctccg
     2041 ccacgccatc cagatcatat ggattgacca ataaggcatc ccgtcgcaat tgcacggccg
     2101 cgccggcgaa ctcgctcagg atcagaacgc cgtcaccgtc gagctggcag gcacagtact
     2161 ccttggccac caggttcatg ccatctttca gaggcgtgac cagcgccacg tcggccgcgc
     2221 agtaatatgc caacagctcc tcgcgctcca ggctgcgaaa tacgtagtgt attgggaccc
     2281 agccgggctg cgtaaagccg ccgttgatct gggtgacgag ccgctcgatt tcggcccgca
     2341 gctcctggta ctccggcacg gtttcgcggc tgggtacgac tacctgcagg agcgtcacct
     2401 tgcgatgcag gtcctcaaaa cgctccaggg cccgcccgaa agcccgcagc cggtatggta
     2461 tgcccttggt gtagtcgagc cggtccaggc ccaggacgag ctgctcgccc ggcatttcgc
     2521 ggcggatctc ttcggcgtgt gtggccacgg agggcagcgc cgcgccctgc gccaacgcct
     2581 ggtagtcgat tccgatcggg aagtgacgca caaacgtcgt ttggccgccc caccgcactt
     2641 cgagcccgcc atggcgccgc cgctggcgcg cgtccggcag tagatcgcgc acgcagtcgg
     2701 cgaagttctt ccggtcgcgc ggcgtttgaa agccgagaac gtcgtatttg agcaggcctt
     2761 ccagcacgtc cacgcgccag ggcagcttct cgaagatgtc cggcggtgga aacgggatgt
     2821 gcaggaagaa cgcgagccgg ttccgcaagc cgcgccggcg aagctggcgc cccagcccca
     2881 tcaggtgata atcctgcacc cacagcaggt cgtccggctg ggcgtgctgg gccacgatct
     2941 ccgcgaactt gtcgttaacc gccaggtagg tcgtccaata gtccggcacg aaattgcaga
     3001 acgactgcaa atcgtgaaac aggggccaga taatctcgtt tgagaatccg cggtagaagc
     3061 cctccacctc ctggcttgtc atggctacgg gtatcagctc aaaaccagca tcgcgctggt
     3121 actcgtccaa cgtgcgctgc agctcatccg tctggtccac gtcactggcg ccggaccagc
     3181 cgatccacac accgctctgg cgttgcagga tcggcgtgag cgctgcaatt aatccgcccg
     3241 cgcctggctg cgtccgccag gcgccgtcca ccatttccag cacgagcggc aggcgatttg
     3301 agactatcac caagcgcatg accacctcac ttcgccccct ccttccggcg tcgcgacaaa
     3361 cgcgcgctcc ccgaatccct accaggacat gttaagcgca acgccgatat tagtcttgtc
     3421 ggcgcggccc cggaacgccg gctggtttcg ccggtggaca atgtcagcgc cggcgcgcgt
     3481 ccacacgcat cacgcggcgt cgcgatacag gttgagcgcc gttcctagcc gccgcgcacc
     3541 atcttgtggt atgccgccat cagctctttg gtcatcgatc ccggtacgcc ggcccctatc
     3601 ttccggcggt ccacttcgat tagcggcacg atttccgcgg ccgtacctgt cagaaacgcc
     3661 tcgtcggccg tgtacaggtc cacgcgttcg aggttcttct cgacgacctc gatcccggtc
     3721 tcacgcgcca gccggatcac gtgcccgcgc gtaatgcctt ccagaatccc ggctgacgtg
     3781 ggcggggtct gaagctggcc gttgcggacg acgaaaacgt tgtccgcggt ggcttccgcg
     3841 atatagccga ggtggttgag catgatcgcc tcgtccacgc cggcctggtg cgactcgatc
     3901 ttggcgagga tgttgttcag gtagttgagc gacttaatcc gcggcggcat cgaattgggc
     3961 aggttccgca cgaccgaagc cacaatcgcc cgcatgccct gctcgtaaac ctcgcggcgg
     4021 tagatgccca gcggcgtggc gatgacgaac acgtttggcc ggccggcgcg cgtgggcgac
     4081 atgcccagca tgccgacgcc gcgcgtggcg acgagccgca aatacgcgtc cttgtccggc
     4141 gtgcccagcc cgttggcatc cagcgtggtg gtgaacgcct gctcgatgtc ctccggctga
     4201 taaggcaggt ccagcatgat ggccttggcc gagtcgaaga agcgccgcag gtgcccggcg
     4261 gcgtcaaaga cgcgcccgtt gtagctgcgc agcccctcga atatgccgtc gccatagagc
     4321 aggccatgtt cgaagatgct gagcttggcg tcggcggcgg taatcaggtt gccgttgagc
     4381 cagaccttca ggttcggatc ggggtcgaag ttctgtgggg cggtcgccgt ttccatgcta
     4441 aacctccgct tagggctttg gagcggggca g
//
LOCUS       DAOSYS010000281         4460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_15994_length_4460_cov_4.033598, whole genome shotgun sequence.
ACCESSION   DAOSYS010000281 DAOSYS010000000
VERSION     DAOSYS010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15994_length_4460_cov_4.033598"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..2133
                     /locus_tag="PLU99_13830"
     CDS             352..2133
                     /locus_tag="PLU99_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO18170.1"
                     /translation="MAEISSSAQGGGDERMVSAETLSSRTAPPPHSTAWRLGKQYAES
                     WPVSSDPKKDAARTSDGYVNSAQYLSAAQRQRSQHSRDAGHNGDAAGPGSFRGVDLDY
                     HHWLEDVLGKRPVRWLLGFLSRPRRDGGTIIQQAIESYANPQASLWQRFKYWPIHIAI
                     RRIKGATSDETFRRSVAEHTSTVRGLVLTARSVAEFGLTTPQRFSAPMFLVWNFTNLC
                     NLNCRHCYQDTAHRRLPNELLLAEKLNVVDQAGRLSIPMIAFAGGEPTIDRDLLPVLH
                     RCHKYGIHTSIATHGGTMTPKLAAELAEAGVRYVEVSLDSVDARRHDEFRGVRGMWER
                     SVRGMRIVTQTPGLRLGVAMCVHQGNYDEVERMIEFAIDIGASTFAHFNFIPVGRGLE
                     MVEGDLTPRQREQLLLLLNKWMQSGKIGVLSTAPQLGKVCLAHAPIEGRTAASHAGSG
                     AGLKARVLAKYLGGCGAGRCYNAIQPNGDVTPCVYLPHRVLGNVRQRPLAEMFRDNNF
                     WEITCDRARRLSHCEVCKFKNYCGGCLARSDAYYGVLNAGDPGCLFNEKHWQELVRRG
                     VASDPTLANGCDGEAVEARAGAVQATQ"
     gene            complement(2157..2714)
                     /locus_tag="PLU99_13835"
     CDS             complement(2157..2714)
                     /locus_tag="PLU99_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOO18171.1"
                     /translation="MTRDEAWNLLCEYTQNDSLRKHALAVEAAMRHYARHFGEDENLW
                     GVVGLLHDFDYERWPNPPAHTREGARILREKGVDEEIVGAILSHGHWNLDEYPRDRPL
                     RKTLFAVDELCGFLIACALVRPTRLEGLEPKSVKKKMKTPAFAAAVSREDIEAGAQLL
                     ELPLDDHIRNCIAALQPHAAALGLG"
     gene            complement(2775..3482)
                     /locus_tag="PLU99_13840"
     CDS             complement(2775..3482)
                     /locus_tag="PLU99_13840"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18172.1"
                     /translation="MTYRDWLMQGEQACTEKKYDEAIRHLTQYINAAGGSPEAARAHY
                     LRGVALAELSRRQDARQDLQRAAAFPGQADVCWRANSVLGTLDYEDGRWAEAAKYYEA
                     VAEIAPQEPPKDLFLFRLGACYERMGRWETARRTFRRIVDEFPQSSVVSDAVRRTQIN
                     ADHFSVQCGVFSSIKNAEGLVKQLQQAGFSPTVQREPRGGVLVYVVRVGRFNNYEMAI
                     QELARVKGYVPTAVLWP"
     gene            3681..>4460
                     /locus_tag="PLU99_13845"
     CDS             3681..>4460
                     /locus_tag="PLU99_13845"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOO18173.1"
                     /translation="MDPLTHGILGGVTAYAAVGGRTPWAAVVGVLTGMAPDLDVFIRP
                     NGDPLGGLSYHRSFTHALVVVPLGAALCALPFLPWRAFAGRAWLVLVAALVAYGAHGL
                     LDAFTSYGTELGWPFTRARVAFDTISIIDPLFTAVLLVGLISALVAGKPNLARAALGV
                     AALYLAFGGLQHFRGARAQRDLANIRGHTLVRGRVMPTLGNLVIWNSVYESDGRLYTD
                     SLRLPIVGQPQVRVGSSGERVTLETLRQRGHDSPVVTAAFER"
BASE COUNT          776 a         1520 c         1372 g          792 t
ORIGIN      
        1 tcatcggcca gcggctgctc gacctgctcg acggacattg tgaattgcac gtttcggggt
       61 ggccgaagcg cgcccatctg gaaatcgtga cccacaatag ccggttagaa gggcggaagg
      121 cgaatttcca ggttcaggaa tcaggagggg gacagactca cccggcgtgc gaaagctgac
      181 agccgggtcc cgcgccgaag cgcctgccgc gagcatgccg ggccggattg tcaggtttcg
      241 tcactccgca ctcccgcaga catggacttg tctcgcatcc gctgaataat cctcgtatat
      301 ttggcgttct ctgtttgccg ggcgatggag cgcgggccgc tgggagacga catggccgaa
      361 atttcttctt ccgctcaggg cggtggtgac gagcggatgg tgtctgctga gacgctctcg
      421 agtcgcacgg cgccgccgcc tcattccacc gcctggcggc tcggcaagca gtacgccgaa
      481 agctggcccg tttccagcga tcccaaaaag gatgccgcgc gcacctcgga cggatacgtc
      541 aattccgccc agtacttgag cgcagcgcag cggcagcgca gtcagcacag cagggatgcc
      601 ggtcataatg gagacgccgc cgggccgggc agctttcgcg gggtggatct cgattatcac
      661 cactggctgg aggacgtgct cggcaagcgc cccgtgcgct ggttactggg cttcctgtcg
      721 cgcccgcggc gggacggtgg cacgatcatt cagcaagcga ttgagtcgta tgccaatccg
      781 caggctagcc tgtggcagcg tttcaagtat tggccgatcc acattgctat tcgccgcatc
      841 aagggggcca catcggacga gacgttccgc cgcagtgtgg ccgagcacac cagcaccgtg
      901 cgcgggctgg tactgacggc ccgcagcgtc gcggaattcg ggctcaccac gccccagcga
      961 ttctccgccc ccatgttcct ggtatggaac tttaccaacc tttgcaacct gaactgccgg
     1021 cactgctatc aggataccgc gcaccgccgt ttgcccaacg aactgcttct ggcggagaag
     1081 ctgaacgtgg tcgaccaggc cggccggctg agcatcccca tgatcgcctt cgccggcggc
     1141 gagccgacca tcgaccgcga tctgctgccg gtcctgcacc gctgtcacaa gtacggcatt
     1201 cacacgagca tcgccacgca tggcggcacg atgactccca aactggccgc ggagctggcc
     1261 gaagccggcg tccgatacgt cgaggtttcc ctggattctg tcgacgcgcg gcggcacgac
     1321 gagttccgcg gcgtgcgcgg catgtgggaa cgctcggtac gcggcatgcg cattgtgacg
     1381 cagacgcccg ggctgcgcct gggagtcgcc atgtgcgtgc accagggcaa ctacgacgaa
     1441 gttgagcgaa tgattgagtt cgccatcgac attggcgcct caacatttgc gcacttcaat
     1501 ttcattcccg tcggccgcgg actggaaatg gtcgagggcg acctgacgcc gcgtcagcgc
     1561 gagcagctcc tgctgctgct gaacaaatgg atgcaaagcg gcaaaatcgg cgtgctctca
     1621 acagcgccgc aactgggcaa agtctgcctg gcgcacgcgc ccatcgaggg ccgcaccgcc
     1681 gccagccatg ccggctcggg cgccggcctt aaggcccgcg tgctcgccaa gtatctcggc
     1741 ggctgcggcg ccgggcgctg ctacaacgca atccagccca acggcgacgt caccccctgc
     1801 gtatacctgc cgcaccgagt actgggcaac gtccggcagc ggccgctggc cgagatgttc
     1861 cgcgacaaca acttttggga aatcacctgc gaccgcgccc ggcgtttgag ccactgcgaa
     1921 gtctgcaagt tcaagaatta ttgcgggggc tgcctcgccc gctccgacgc gtactacggc
     1981 gttctaaatg ccggagaccc gggctgcttg ttcaacgaga agcactggca agaattggtg
     2041 cgccgcggag tggccagcga ccccacgctg gcaaacggct gcgacggtga ggcagttgaa
     2101 gcccgcgccg gcgccgtgca agccacgcag tagccgtaac aggactcgaa acgccgctac
     2161 cccagcccga gcgccgccgc gtgcggctgc agtgccgcaa tgcaattgcg aatatggtcg
     2221 tccagcggca attccagcaa ctgtgccccc gcctcgatgt cctcgcgcga aaccgccgcc
     2281 gcgaacgccg gcgtcttcat ctttttcttc acgctcttgg gctctagccc ctccaatctc
     2341 gtcggccgca ccagcgcgca ggcaatcaga aaaccgcata gctcatccac cgcgaacagc
     2401 gttttgcgca gcggccgatc gcgcgggtac tcgtccaggt tccaatgccc gtgcgataga
     2461 atcgcgccca cgatctcctc atcgacaccc ttctcgcgca gaatgcgggc cccttcgcgc
     2521 gtgtgcgcgg gaggattcgg ccagcgctca taatcaaaat cgtgcagcag gcccaccaca
     2581 ccccacaagt tttcatcctc cccgaaatgc cgcgcgtagt gccgcatcgc cgcctccacc
     2641 gccagcgcgt gcttgcgcag tgagtcgttc tgcgtgtact cgcacaacaa attccaggcc
     2701 tcatcacgag tcataggaag ctcctatggg gaaggaaacc aggcaaacgg actcctgtca
     2761 cattatcgcc gggctcatgg ccacagcacc gccgtcggca cgtatccttt tacccgcgcc
     2821 aactcttgta tcgccatctc gtaattgttg aaccggccga cccgcaccac gtataccaga
     2881 acaccgccgc gcggttcgcg ttgcacggtg ggcgagaaac ccgcttgttg cagttgcttg
     2941 accaggccct ccgcgttctt gatgctcgaa aaaaccccgc actgcaccga aaaatggtcc
     3001 gcgttgatct gcgtccggcg aactgcatca ctcacaacgc tgctctgcgg aaactcgtcg
     3061 acaatccgcc ggaaagtacg ccgggcagtc tcccagcggc ccatccgttc atagcaggcg
     3121 cccaaccgga ataagaacag gtccttcgga ggctcctgcg gcgcgatctc ggccaccgct
     3181 tcgtagtatt tggccgcctc ggcccagcgc ccgtcctcgt aatccaacgt ccccagcacg
     3241 ctgttcgccc gccagcacac gtcggcctgg cccgggaacg ccgcggcgcg ctgcaggtcc
     3301 tgccgcgcgt cctgccgccg gctcagctcg gccaatgcca cgccgcgcag gtagtgagcc
     3361 cgcgccgcct ccggcgagcc gccggccgcg ttgatgtact gcgtgaggtg ccggattgcc
     3421 tcgtcgtact tcttctcagt gcaggcctgc tcgccttgca tgagccagtc cctgtacgtc
     3481 atggagggtt cgcagccgca ggccagcagc aaacagcccg tcaccgatag gagaattagc
     3541 cttcgcatgc ccggaagtta acgcactcac cggcaggcgc gaagtacgcc cgcgcaaatt
     3601 gactgccgcg cccgcccggc tagcatcagt gaccgctgac ttctccggcc aagggcggag
     3661 cgcagggaca gagcaagcaa atggatcctt tgactcacgg tattttggga ggagttaccg
     3721 catacgccgc cgtcggcggc cgcaccccct gggccgctgt cgtcggcgtg ctgaccggca
     3781 tggcgcccga tctggatgtg ttcattcggc ccaacggcga cccgctgggt gggctcagct
     3841 atcaccggtc tttcacacac gccttggtcg ttgtgccgct cggggcggcc ttgtgcgccc
     3901 tgccctttct gccttggcgg gccttcgcgg gcagggcctg gctcgtcctg gtggccgcac
     3961 tggtcgcgta cggcgcgcac ggcctgctgg acgcgttcac cagctacggc acggagctgg
     4021 gctggccctt tacacgcgcc cgcgtggcgt tcgacacgat cagcatcatc gatccgctct
     4081 tcaccgcggt gctgctggtt gggctgatct cggccttggt cgcgggaaag ccaaatttgg
     4141 cacgcgcggc tttgggggtc gccgcgctat acctggcgtt cggcggcctg cagcacttcc
     4201 gcggggccag agcgcagcgc gacttggcca acatccgcgg ccacacgctg gtgcgtgggc
     4261 gcgtgatgcc cacgctcggc aatctcgtca tttggaactc ggtgtacgag tccgatggcc
     4321 ggctttacac ggattcgctg cgtttgccga tcgtcggcca gccgcaggtg cgcgtcggct
     4381 cgtcgggcga gagggtgacg ctggaaacgc tgcgccagcg cggccacgac tcgcccgtcg
     4441 tgaccgcggc cttcgagcgg
//
LOCUS       DAOSYS010000282         4435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16096_length_4435_cov_4.623516, whole genome shotgun sequence.
ACCESSION   DAOSYS010000282 DAOSYS010000000
VERSION     DAOSYS010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16096_length_4435_cov_4.623516"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..403)
                     /locus_tag="PLU99_13850"
     CDS             complement(68..403)
                     /locus_tag="PLU99_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOO18174.1"
                     /translation="MAIKRTIHPRERYEARAAVAKALAHPTRLLLLDALAEGELCVCE
                     LNRLVPADQSTVSKHLAILKQAGLVAERREGPKIFYRQTVCCLKGLWECIESVLKQNL
                     KAQQAAIAS"
     gene            923..1321
                     /locus_tag="PLU99_13855"
     CDS             923..1321
                     /locus_tag="PLU99_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOO18175.1"
                     /translation="MSGGARKLRVHPLEPITTRGELTLDDGTVCAIVPKEQLERLLRQ
                     AELQEMAAMLEDPNTEWADLDDYKLQVAGSTIAAARKARGLTQAQLARQLKLPQSQIS
                     RIERHPDRTTVRTLKKIAAALKVNVRQLLP"
     gene            complement(1458..2846)
                     /locus_tag="PLU99_13860"
     CDS             complement(1458..2846)
                     /locus_tag="PLU99_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOO18176.1"
                     /translation="MSPLKPVSLNLNVRGMGLSATLAIKQKCRELQQQGLNIYDFGLG
                     QSPFPVPPSVVEALRSAATERDYLPVQGLPALREAVADYHRRTDGIEIHPDDVLVGPG
                     SKELMFQVQLVFYGEVILIPPCWVTYWPQARILGRRLSRIHATWESNWKIDAARLFEA
                     VQRVGDDHRPRLLVLNYPSNPTGDTYSADELREIAEVARRFQIIVLSDEIYGQLHFTN
                     EHVSIARFYPEGTIVSSGLSKWCGAGGWRLGTFSFPRELSWLAKAMATVSSETFTSVC
                     APIQHAAIRAFQGGPDIEQYLRHARRVLSALGDYCARTLAAAGLNVRRPRGAFYLFPD
                     FTPLAAKLAARGITDGKTLCDRLLDEAGVAVLPGSAFGRSRGELTARLSYVDFDGATA
                     LQASEGTPSEAGEATPREAGEATPREASEDTLREAGETTPCEAPLTEAFLRRHCDRVV
                     TGIDKLAAWVQA"
     gene            complement(2982..3896)
                     /locus_tag="PLU99_13865"
     CDS             complement(2982..3896)
                     /locus_tag="PLU99_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18177.1"
                     /translation="MHAREQPQELLQQVVAALQGGQTQHAADLLISAHAAGTLPERTA
                     MLPELARRVSPNLVAELLLQMATFPCFYCRVGLQSCEACEGRGRLGDDLPCERCVGLG
                     VARCDFCDGTGWTSLEALPPSLRPLIILQRVRLALRASRKLLAQAPAPLEHAGEREAR
                     RAAARQVLAVERQRAILDDALATAERAGVNSRLGKELEKLLPACAACVPRVESRLREC
                     LSNLADVSRREAEREDADPHSRRRARSRVSYYDQLRRSGNFAGTGLERLQLSQVARRI
                     LRQARDAAQKDNGNTSSGGPTAENPVPQ"
BASE COUNT          753 a         1404 c         1479 g          799 t
ORIGIN      
        1 agacgccaaa ccctggtggc ggggccggct cgttggtgcg ccggggtctg tacgggcgcc
       61 gtaacgctca tgatgcgatc gccgcctgct gggccttgag gttctgcttc agaaccgatt
      121 cgatgcattc ccacagccct ttcaggcagc agacggtctg gcggtagaag atctttggac
      181 cttctctgcg ttccgcgacc agtccggcct gcttgaggat ggccaggtgc ttggaaacgg
      241 tggactggtc ggcgggaacc aggcggttca gctcgcagac gcacagttcg ccctcggcca
      301 gcgcatccaa caggaggagg cgggtcgggt gggccagggc cttggccacg gcggcccggg
      361 cctcgtagcg ctcgcgcggg tggattgttc gcttaattgc catatggcca tattgccata
      421 aagccagact cggcgtcaag gctcgcatgt tggaaaaacg ggctgaccgg tcgcttgaca
      481 gtatggcgaa aatgagatat tcttttatat cgaaaataag atcgagcccc ggatcaacgt
      541 ctggtagaac acgacagggg caagccggag gccgctacgc gacgtgttct accttgatca
      601 gcgctaaacg tcggccacga ggtggcggga gtttcaatgc gcatcaaggt agaactcagc
      661 cggaatgcga tcgccttcct cgaccagctt gctccggttg atcgggatga ttttttcgcg
      721 gagctcgata ggttgcgagc cggtccgagt gagctgctcc gtttgtcaga gcaatattcg
      781 gatcctagca tcagccgata tgtgctgagg cgatttgcgt ttggtcgtgg cgtggcgaag
      841 actgccatct ttgaatacga cgcggcccgc gcacgcatgc gggtgttgta tgccggctgc
      901 aaaggccaag agaaatgcgt ccgtgagcgg gggagccagg aagctacggg tacatccgct
      961 ggagccgatt actacccgcg gcgagttgac gctggacgac ggcacggttt gtgcgatcgt
     1021 cccgaaagaa cagctcgagc ggcttctgcg gcaggccgaa ttgcaggaaa tggcggctat
     1081 gctggaggac cccaatacgg agtgggccga tctggacgac tacaagctgc aggtggccgg
     1141 ttcgaccatc gccgcggctc gcaaagctcg cggcctgacg caggcgcagc tcgcccgcca
     1201 gttaaaactg ccgcagtcgc agatttcccg gatcgagcgg cacccggacc gaaccacggt
     1261 ccgcacgctc aagaagatcg cggcggcgtt gaaggtcaac gtgcggcagt tgctgccgtg
     1321 aagcgtcccc ttcccacgtt cccaccttca cactttccca cttttccact cgcgcacagc
     1381 ggcatgcccg cgcttacagc ttgggcatgg cacccggcac ccgccttggg catggcactc
     1441 aacgttgttc ggtctgatta cgcttggacc cacgcggcca gtttgtcgat tccggttacg
     1501 acacggtcgc agtgtcggcg gagaaaggct tctgtcagcg gcgcttcgca gggcgtagtc
     1561 tcgccggctt cccggagcgt atcctcgctg gcttcgcggg gcgtggcctc gccggcttcc
     1621 cggggcgtag cttcgccggc ttccgagggt gtgccctcgc tggcctggag cgcggtcgct
     1681 ccgtcgaaat cgacgtagga cagccgtgcg gtcagctcgc cgcgcgagcg accgaaggct
     1741 gagcccggca agaccgccac gccggcttcg tccagcagcc ggtcgcacag cgttttgcca
     1801 tccgtgatgc cgcgcgccgc cagcttggcc gcgagcggcg tgaagtcggg gaacaggtag
     1861 aacgcaccgc gcgggcggcg cacgttcagg ccggccgcgg ccagcgtccg ggcgcaatag
     1921 tctcccagcg cgctcagcac gcggcgtgcg tgccgcaaat attgttcaat atccgggccg
     1981 ccttgaaagg ccctaatcgc ggcatgctgg atcggagcgc agaccgacgt gaaagtctcg
     2041 ctgctcacgg tggccatcgc cttggccagc cagctcagtt cgcgcgggaa gctgaaggtc
     2101 ccaagccgcc agccgcccgc accgcaccac ttggagaggc ccgaactaac gatggtgcct
     2161 tcgggataga agcgggcgat cgatacgtgc tcgttagtga agtggagctg gccgtaaatc
     2221 tcatccgaca agacgatgat ctgaaaacgg cgcgcgactt ctgcgatctc gcgcagctcg
     2281 tcggccgagt atgtatcgcc cgtgggattg ctggggtagt tgagcaccag caggcgcggg
     2341 cggtggtcat cgccgacgcg ctgcaccgcc tcgaacaggc gtgcggcgtc gatcttccag
     2401 ttggattccc aggtggcgtg aatgcggctc aggcggcggc cgagaatgcg ggcctgcggc
     2461 caataggtga cccagcaggg gggaatcagg atgacctcgc cgtagaagac gagttgcacc
     2521 tggaacatca gctctttcga acccggaccg acgagcacat cgtccgggtg gatctcgatg
     2581 ccatccgtgc gccggtgata gtcggccacc gcctcacgca gcgccggcag accctgcacg
     2641 ggcaggtaat cgcgttcggt tgcggccgag cgcagggcct cgaccacgct gggcggcact
     2701 gggaagggcg attggcccag gccgaagtcg tagatattca gaccctgctg ctgaagctca
     2761 cggcacttct gcttaatggc gagcgtggcc gatagcccca tgccgcggac gtttagattg
     2821 agactcactg gttttagcgg ggacacgcag cgctcccgtt gcaccttgta cggcgcctgg
     2881 gccggaaagt cagctcattt caccgcttcc gagcgggcgc ggcaagccgg gtgtgggtcg
     2941 agcaaaaccg ccaggacggc tggcctgccc gccgctttgg actactgtgg gacggggttt
     3001 tcagcggtgg gcccacctga gctcgtgttg ccattgtcct tctgagccgc gtcccgtgcc
     3061 tgccgcagga ttcggcgcgc tacttggctc agttgcaggc gttccaggcc ggttcccgcg
     3121 aaattgccgg aacgccgcaa ctggtcgtaa tagctcacac gtgaccgggc ccgccggcgc
     3181 gaatgtggat ctgcgtcctc acgttcagct tcccgccgcg acacatcggc caggttactc
     3241 aggcattccc gcaggcggga ctcgacgcgc ggcacgcagg ccgcacaggc gggcaacagc
     3301 ttttcgagct ctttgccgag gcgcgaattg accccggccc gttcggccgt cgccagcgca
     3361 tcatccagaa ttgcgcgctg cctttcgacc gccaggacct gccgggccgc cgcgcgccgc
     3421 gcctcacgct caccggcgtg ctcaagcggg gccggagctt gcgccagcag cttgcggctg
     3481 gcgcgcaggg ccaatctaac tcgctgcagg attatcagcg gccgcaggga cggcggcagg
     3541 gcttcaaggc tcgtccagcc ggtgccgtcg cagaaatcac atcgcgctac gccaaggccc
     3601 acgcagcgtt cacacggcag atcgtcgcca aggcggccgc ggccctcgca ggcctcacag
     3661 ctctgcaggc ccacgcggca gtaaaagcag ggaaaggtgg ccatttgaag gagcagttcg
     3721 gccacgaggt tcgggctgac gcggcgcgcc agttcgggca gcatggcagt gcgttcaggc
     3781 aatgttccgg ccgcgtgggc ggagatcagc aggtctgcgg catgttgcgt ctgtcccccc
     3841 tgcaaggcgg cgactacctg ctggagcagt tcctgcggct gctcgcgagc gtgcacaacg
     3901 ctcccgctcg ccccctggtg ggccgagttg tctgctccgc cgctgtgcat gtagtcacct
     3961 gttgttcacc gggcgtccgc gataaccgaa acatcgcgcc ggcccggggc cgcggtcgtg
     4021 ctagcgcatg acggtccgta tgtccttcca cgtcagtgcc cgaacggccg ccaagcaaat
     4081 tcccgctcct tccgcgcata ctgcgcggtt accgcagtcc ttattgatat tacgccggcc
     4141 gatcgacgcg gtgcgggctt aactgcgaat gacctgctgg ttagggtgcg ggtggggggc
     4201 gagactgctg tggagcggga gttcagtggg ccgagccgcg cttcagcggg agcctcgccc
     4261 gcgcacgccg cgcgttcccg cagcgaaact caagccggcc gggcctgctc caaggcgcgc
     4321 cggaagctct cgagctccgc tggtgtatcg aagacgtcct ccagcggcac gtccggctcg
     4381 tcgccggtca gttccagttt tgggacgcgg ccgcgcgtgt tgaggcggtg aatct
//
LOCUS       DAOSYS010000283         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16254_length_4401_cov_5.145421, whole genome shotgun sequence.
ACCESSION   DAOSYS010000283 DAOSYS010000000
VERSION     DAOSYS010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16254_length_4401_cov_5.145421"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(884..2836)
                     /locus_tag="PLU99_13870"
     CDS             complement(884..2836)
                     /locus_tag="PLU99_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOO18178.1"
                     /translation="MGLIRVQNVTKQFGGQVVLNDVSVEFSTGHVVGLVGPNGAGKTT
                     LFKLITGQLAPDLGTVTLSKGLEVGYLPQEPEVAEGQTLHDEVLSVFADVLALEQKLQ
                     GISEQMAAQPAGPALDELMQQYDRTTARFEAAGGYTYEQRLGEILGGLGFSQADYKLP
                     MAALSGGQKCRAALAKLLLQESQFLLLDEPTNHLDIDAVRWLEKFLAGHHGGAVIVSH
                     DRYLLDRLAERIVEVEGARLRSFPGNYTNYVQTKAVRQLTQDRQFVNDRAFIQKEREF
                     IAKHMGKQRTAEAKGRLKRLERRLAAGEFTLERTSQREKIKIRFGEADRQVTAGKDVL
                     SVRGLSKAYGEKRLFTDLSLTVPAGSRLGITGPNGTGKTTLLKIVLGQVRADAGEFDL
                     DVKASVGYYAQEATELDPNRTVVQEILTVRQEWLESHARSYAARFLFRGEDPFKRVSQ
                     LSGGEQSRVRLMKIILANPNVLILDEPTNHLDIASREVLEEALLEFPGTIIAVSHDRY
                     FLDRICDHLLVMRSNEHALYGGNYSYYIRQVEAERESAAMAAGAEKRPKAAKGGKTPR
                     GGGQDAAAAVEKPQRTRFHKLRLEELEQLIIDLETRLMELHERFGEPELYHDATGVSE
                     LREELDAVQAELAEAEQEWNARAERE"
     gene            3061..>4401
                     /locus_tag="PLU99_13875"
     CDS             3061..>4401
                     /locus_tag="PLU99_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOO18179.1"
                     /translation="MQRSLIHAAILVLNLSLATAAWAAPGDVRKAFDLPCKYPAGLAS
                     DGASLYVVDWREPKIYRLSADDGGVQQTWPAPTLKPRGLAYGGGRLYISDDHNGSIYA
                     LDPQTGIVEDTFAAPDKQAAGLAYADGVLFVLERKTKTIYKVTPDDGTILATCPVPDR
                     QCEALGWDGKYLWTANRVRNEIYMLEPESGAVLNILDAPGPYVAGLAFINGQLWADDF
                     QTRKLYQVATDDEPYYRLSEPRQERCEFYWALYNYGPGEIRDLTVNLAVPTDLVNQQI
                     LSAPAFSRPPVQTRPDKWGQQCALFECGTVPAGEKAVISYAADVRLSALRYFILPEKC
                     GTLADIPAEIKTAYAVDDPQRLGVESRYIKDLAAKIVAEEQNPYWIARKVYDYLAEHL
                     EYEMVGGWDNPEVVLKRGKGSCSEYTFAFIALCRAAGLPARYQGSVVVRGDDASV"
BASE COUNT          780 a         1429 c         1351 g          841 t
ORIGIN      
        1 tcaagcggtc tgataaaaag tcgcgtggaa tcgggccatg ggggcaggtg atcagatact
       61 catggttttt gtgcacacga ttttgataac ctgaggcccg ccgctgcgcg gagagaggag
      121 gccgctgcgc ggagagagga gataggtgag aggtgaacag gtgaacaagg ccgctgcgcg
      181 gagacggatg atagttgaca ggtgagtagt ggggacgttt tgaatgccgc atagtgggtg
      241 cctttctgcg cgcgggcgcg gttctgctag ggattctata aggagctgtc agctttcagc
      301 tatcagcttt cagccagaat gcgtggattg tgcatgtatg cgcgccaatg gcggcgcgag
      361 cgttgcttgg ggtgataaag tgataacgtg atagggtgat aaagaacttc cagcctttca
      421 gctattagct attagctttc agccagatcg cgccgaacaa ccagacgatg cggcgcggtt
      481 tcacacgccg ggcccatttc acactccgaa gggatctagg gatcgaggga tcgagggatc
      541 aagagctgtc agctatcagc tctcagcttt cagccagatc gcaccgaacg accagtggcc
      601 cgcgatgctc ggtcgcattt accccgaatc ggccccggtg acgcactcgt ccgcaattcc
      661 ggccactgct ggaacagtag tggcacgcta gcccgagcgc gcgtgctcgc agccccgttt
      721 aatacgttgc gctcgtacta atctaacgca tcctcgggag cgccgaccat acgcttgcgg
      781 caccgggcca aggccgcgcc atatctggcg gttgcttacg tattgacagt tgccttgcgt
      841 cttgtccgtt gccttgcgtc ttggcagttg ccttgcggcc ggattactcc cgctccgcgc
      901 gcgcattcca ttcctgctcg gcctcggcca gttcggcctg cactgcgtcc aattcctcgc
      961 gcagttcaga aacgccggtt gcgtcgtgat acaactccgg ctcgccgaac cgctcgtgca
     1021 gctccatcag ccgcgtttcc aggtcaatga tgagctgctc caattcctcc agccgcagct
     1081 tgtgaaagcg cgtccgctgc ggcttctcca ccgccgcggc cgcatcctga ccgccgccgc
     1141 gcggcgtctt gccgcctttg gccgctttcg gccgcttctc cgcgccggct gccatggcgg
     1201 ccgattcgcg ctccgcctcg acctggcgga tgtagtacga atagttcccg ccgtacagcg
     1261 cgtgctcatt ggaccgcatc accagcaggt gatcgcaaat gcggtccaga aaataccggt
     1321 cgtggctgac cgcgatgatc gtcccgggga actccagcag cgcttcttcc aggacttcac
     1381 gcgatgcaat atccaggtga ttcgtcggct cgtccagaat cagcacgttc gggttggcca
     1441 ggatgatttt catcagccgc acgcgcgact gctcgccgcc cgagagctgg ctgacgcgct
     1501 tgaacgggtc ctcgccgcgg aagaggaagc gggccgcgta actgcgggcg tggctttcga
     1561 gccactcctg ccggaccgtg agaatctcct gtacgaccgt tctgttcgga tcgagctccg
     1621 tcgcctcttg cgcgtaatac ccgacgctgg ccttcacatc caaatcgaac tcgcccgcat
     1681 ccgcccgcac ctgccccagt acgatcttca acagcgtcgt cttgcccgtg ccgttcgggc
     1741 cggtgatgcc cagccggctg cccgcgggaa ccgtcaacga caggtccgta aacagccgct
     1801 tctcgccgta cgccttgctc aacccgcgca cgctgagcac gtccttgccg gcggtgacct
     1861 gccggtcggc ctcgccaaag cggattttga ttttctcccg ctgcgatgtg cgctcgagcg
     1921 tgaactcgcc cgcggccagg cggcgttcca ggcgtttgag acgtcccttg gcctccgcgg
     1981 tgcgctgctt gcccatgtgc ttggcgatga actcgcgctc tttctgaatg aacgcgcggt
     2041 cgttgacgaa ctggcggtcc tgcgtgagct ggcggactgc tttcgtctgc acatagttcg
     2101 tgtagttgcc cggaaacgag cgcagccgcg ccccttccac ttcgacgatc cgttccgcca
     2161 ggcggtcgag caggtagcgg tcgtgcgaaa cgatgaccgc cccaccgtga tgtccggcca
     2221 ggaacttttc cagccaacga acggcgtcga tgtccaggtg attcgtcggc tcatccagca
     2281 ggaggaactg ggactcctgc agcagcagtt tggccagggc cgcacggcac ttctgcccgc
     2341 ccgacagggc cgccatcggc agcttgtagt ccgcctgcga aaaccccaga ccgccgagaa
     2401 tctcacccag gcgctgctcg tacgtatagc cgccggccgc ctcaaaccgg gccgtcgtgc
     2461 ggtcgtactg ctgcatcagc tcgtcaaggg cgggcccggc cggctgggcc gccatctgtt
     2521 cggagatgcc ttgcagtttc tgctccagcg ccagcacgtc ggcgaagacg ctcaagacct
     2581 cgtcgtgcag cgtctgcccc tcggcgacct cgggctcttg cggcagatag ccgacctcca
     2641 ggcccttcga gagcgtgacg gtgcccaggt ccggggcaag ctggccggtg atcagcttga
     2701 acagcgtggt cttgccggcg ccgttcgggc cgaccagccc gaccacgtgc ccggtgctga
     2761 attcgaccga gacgtcgttc agcaccacct ggccgccgaa ctgcttggtc acgttttgaa
     2821 cgcgaataag acccattgcg tcagtttagc acggcggcgg ggtagcgagc agtggcttga
     2881 tgccgatgcg gggtgatggc catgctgcgt gccatctaac gagggcatgc ccgcgcttac
     2941 agcttgggca tggcgcccaa cactttcacc caacactttc acccaacact ttcgcccaac
     3001 actttcgcac gtacaatgcc ggccagtccg agaattgttg aaagagctac ggagcccgtc
     3061 atgcaacgct cgctaatcca cgccgcgatc ctggtcctca acctaagcct ggcaaccgcc
     3121 gcctgggccg cgcccggcga cgtgcgcaag gcgtttgacc tgccgtgcaa gtacccggcc
     3181 gggctggctt cagacggcgc cagcctttat gtcgtggact ggcgcgaacc caagatttac
     3241 cgcctgtccg cggacgacgg cggcgtgcaa cagacctggc cggcgccgac gctcaaaccg
     3301 cgcggcttgg cctacggcgg cgggcggctt tacatctccg acgaccataa tggctcgatt
     3361 tacgctctcg acccgcagac cggaatagtc gaggacacgt tcgccgctcc ggataagcag
     3421 gccgcgggct tggcatacgc ggacggcgtg ctgttcgtgc tggagcggaa aaccaagacg
     3481 atctacaagg tgacgcccga tgacggcacg attctcgcca cctgcccggt gcccgaccgg
     3541 cagtgcgaag cgctgggctg ggatggcaaa tatctctgga cggccaaccg ggtgcgcaac
     3601 gagatttaca tgctcgagcc ggaaagcggc gcggtgctga acatcctcga cgcgcccggc
     3661 ccgtacgtcg cgggcctggc gtttattaac gggcagcttt gggcggacga cttccagacg
     3721 cgcaagctgt accaggtcgc taccgacgat gaaccctatt atcgcctttc agagccgcgc
     3781 caggagcgct gcgaattcta ctgggcgcta tacaactacg gccccggcga gatacgggac
     3841 ctgacggtca atctggcagt gccgaccgac ctggtgaacc agcaaatcct ctcggcgccc
     3901 gccttttcac gccccccggt gcaaacgcgg cctgacaaat ggggccagca atgtgcattg
     3961 tttgagtgcg gcaccgtgcc ggccggcgaa aaggccgtga tcagctacgc cgccgacgtc
     4021 cgcctgtccg cgttgcgcta cttcatcctg cctgagaagt gcggcacgct ggctgacatt
     4081 ccggcggaga tcaaaaccgc ctatgcagtt gatgatccgc agcggctggg cgtcgagagc
     4141 cggtacatca aggacctggc cgccaagatc gtcgcggagg agcagaaccc ctactggatc
     4201 gcgcgcaaag tgtacgacta cctggccgag cacctcgagt acgaaatggt gggcggctgg
     4261 gacaatcccg aagtggtgct gaagcgcggc aaaggctcgt gttcggagta caccttcgcc
     4321 ttcatcgccc tgtgccgcgc cgccggtctg ccggcccgct atcagggcag cgtggtcgtc
     4381 cgcggcgatg acgccagcgt c
//
LOCUS       DAOSYS010000284         4400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16262_length_4400_cov_5.315765, whole genome shotgun sequence.
ACCESSION   DAOSYS010000284 DAOSYS010000000
VERSION     DAOSYS010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16262_length_4400_cov_5.315765"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /gene="ligA"
                     /locus_tag="PLU99_13880"
     CDS             complement(<1..1625)
                     /gene="ligA"
                     /locus_tag="PLU99_13880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOO18180.1"
                     /translation="MDRAAAAERIAVLRAEIQRHDHLYYVEAQPEISDEHYDALLREL
                     AELELQFPDLITPDSPTQRVGERPLEGFEHVEHAVPMLSVDNTYSPDELRAFDQRVRR
                     TLGVETVAYAVDPKIDGVAVSLRYEDGSFVRGATRGDGRVGDDITQNLRVIRSVPLRL
                     RGSSWPHIVEARGEVYWPRPDFEENNRRLAKAGKTPFANPRNATAGTLKQLDPRIVAR
                     RGLAFTAHSFGLIDPFPADVTKSMQLYERFREWGLPISRYLRLCTGIEEVIAFVEEWD
                     SQRKQLDFDTDGLVIKVDDLEQRKRLGSTSKSPRWCIAYKYATEQATTRVLSVGMHVG
                     KMGTITPVANLEPVLVAGTTVRRATLHNFDQVRRLDVREGDTVTVEKAGEIIPQVVAV
                     NTAVRPKTAEPVKPPERCPECGGEVQQDEGGVYLRCINPECPAQRFERLKFFCGRDQM
                     DIALLGEVMVGKLLEAGLLHSYADIYRLEQRRDEVRRLVIEQQRKTDGGSKPVRVEFG
                     EKRTATLLKGIEESKSRPLSRVLAALGIRHVGA"
     gene            complement(1628..2416)
                     /locus_tag="PLU99_13885"
     CDS             complement(1628..2416)
                     /locus_tag="PLU99_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18181.1"
                     /translation="MRVDRMRLPLRLFGTGFRAVRTVVPVFGLVLVLGGCGAGRFGSE
                     LEDRPKPLPVRRAEGITLHIPAEQQFSIALPQSSRQAGLGGTAQSDATVTPAGSADAW
                     AEVTNGGQASGLFQLGHSFANETGQVADFEFVIRLGYAYSASATPETAFADAKVGLKL
                     YARTNMGRLVRDITLFTHETSNGPAQGTASASSQFTLTLLPNEAVNVFIAGQAAVDIR
                     EQRSGAGRVSVSNLAMDVTIRPSASAPRFTPGFVPQTGAGAGQP"
     gene            complement(2592..3071)
                     /locus_tag="PLU99_13890"
     CDS             complement(2592..3071)
                     /locus_tag="PLU99_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18182.1"
                     /translation="MITQLESAKSRMQRFGDEMDARIQQQMQQQIECQVLHYARHPER
                     IEERLRQLDQEWDLGRRFQANAAGIILGSLILGRAFRLLRPLPYIASGFLLMHAVRGW
                     APPVLALRRLGTRTRDEIEKERSALRALRGDFGEMPGDMQLDQKVERALQVAGLPTG"
     gene            complement(3184..>4400)
                     /locus_tag="PLU99_13895"
     CDS             complement(3184..>4400)
                     /locus_tag="PLU99_13895"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOO18183.1"
                     /translation="MVSISRAMANTLLAKAGLPDLATLQQGLDTQRKPLSRDMGLNVK
                     LETGLEPNRVPARNVLGLLPGAGDTSETIVVGAHYDHLGIQPHGNDPTPQIHNGADDN
                     ASGTAGVIELARAASEATKLRRNVLFIAFDGEELGLCGSREFVEHPTVAFDDIRAMIN
                     LDMIGRAGSGKFSMLGAPTAAEFAGLIEKYAGQVGLNCRTGGLMAGGSDHAPFAARRI
                     PVLFAFTGVHKEYHRPEDDWQLIDAEGAAKVLRLCYGVLAELANMESGPAYTEPPPEA
                     REEEEIRPAVEEKKEAGQDASSTQDSDSKPSRAKLRVRLGVIPDVPGDGKPGMLVQTV
                     IEGSPAALAGMQNGDRVMKIAGEEIRDIYAYMRVLEKFDERDETDIVVLRDGREQKLR
                     VKFQAAETKPGH"
BASE COUNT          758 a         1529 c         1296 g          817 t
ORIGIN      
        1 ttcgcaccga catggcgaat gcccagcgcc gccagcacgc gtgaaagcgg gcgcgacttg
       61 ctctcctcga tgcccttcag cagcgtcgcg gtgcgcttct cgccgaattc gacacgcacc
      121 ggcttggagc cgccatccgt ttttcgctgc tgctcgatca ccaggcggcg cacttcatcc
      181 cggcgctgtt ccagccggta aatgtccgcg tacgaatgta acaggcccgc ctcgagcagc
      241 ttcccgacca tcacttcccc caggagcgcg atgtccatct ggtcgcggcc acagaaaaat
      301 ttcagccgct cgaatcgctg cgccgggcac tcgggattaa tgcaacgcag gtacacaccc
      361 ccctcatcct gttgcacttc cccgccgcat tccggacaac gttccggcgg cttcaccggc
      421 tcagccgttt tgggccgcac ggccgtgttg accgccacca cctgcggaat tatttcgccc
      481 gccttctcaa ccgtgaccgt gtcgccctca cgcacgtcca gccgccgcac ttggtcaaaa
      541 ttgtgcagcg tcgcccgccg cacggtcgtg ccggccacca gcaccggctc aaggttcgcc
      601 acgggcgtga tcgtccccat cttcccgacg tgcatcccaa ctgataacac gcgcgtggtc
      661 gcttgctcgg tcgcgtactt gtaagcgatg caccagcgcg gcgacttact ggttgaaccc
      721 agccgcttcc gctgctccag atcatcgacc tttatcacca gcccgtcagt atcgaagtcg
      781 agctgcttgc gctgcgagtc ccactcttcg acaaaggcga ttacctcttc aatcccggtg
      841 catagccgca aataccggct gatcggcaga ccccattcgc ggaaacgttc gtagagctgc
      901 atcgacttag taacgtccgc cgggaacgga tcgatcaagc cgaagctgtg cgccgtaaat
      961 gccagtccgc gccgcgcgac gatccgcgga tcaagctgct tcagcgtgcc ggccgtcgcg
     1021 ttgcgcgggt tggcaaacgg tgttttgccc gcttttgcca ggcggcgatt attctcttcg
     1081 aagtccggcc gcggccagta gacttcaccg cgcgcctcca caatatgcgg ccaactactg
     1141 ccgcgtagcc gcaacggcac actgcggatt acgcgcaggt tctgggtgat gtcgtcgccg
     1201 acgcgcccgt cgccgcgcgt cgccccgcgt acgaacgacc cgtcttcgta gcgcagtgaa
     1261 accgccacgc cgtcgatctt cgggtccacc gcgtacgcga cggtctcaac ccccagcgtg
     1321 cgccgcacac gctgatcaaa cgcccgcagt tcatcgggcg aataagtgtt gtcgaccgac
     1381 agcatgggca cggcgtgctc gacgtgctcg aagccctcga gcggccgctc ccccacccgc
     1441 tgcgttggcg agtcgggcgt gatcaaatcc ggaaattgca gctccaactc ggccagctcg
     1501 cgcagcagag cgtcgtaatg ctcatcactg atttccggct gggcctcaac gtagtacaag
     1561 tggtcgtgcc gctgaatctc ggcccgcaag acggcgatac gctcggcggc ggcggcgcga
     1621 tccatgacta tggctgcccg gcgcctgccc ccgtctgcgg cacgaacccc ggcgtaaagc
     1681 gcggcgcgct cgcggacgga cgaatcgtca catccatggc caggttggac acactcacac
     1741 gccccgcccc cgaacgctgc tcgcgaatgt ccaccgccgc ctgtccggcg atgaagacgt
     1801 tgaccgcttc attgggcagc agcgtcagcg tgaactgcga cgatgcgctg gctgtccctt
     1861 gcgccggacc attcgaggtt tcgtgcgtga atagcgtaat gtcccgcacc agccggccca
     1921 tgttggtgcg tgcatatagt ttcaagccga ctttggcgtc ggcgaaggcg gtctcgggcg
     1981 tggccgaggc gctgtaggcg taccccagtc gtatgacgaa ctcaaagtcg gccacctgcc
     2041 cggtctcgtt cgcgaaagaa tgcccgagct gaaagagccc gctggcctga ccgccattcg
     2101 tcacttccgc ccatgcatcg gcgctgcccg ccggcgtgac ggtggcgtca gactgggcgg
     2161 tcccgcccaa cccggcttgc cggctcgact gcggcagcgc gatcgaaaac tgttgctcgg
     2221 ccggaatgtg cagcgttatg ccctcggcgc gccgcaccgg cagcggcttc gggcgatcct
     2281 ccagctcgct accgaaccgc cccgccccgc agccacccaa gacgagcacc aggccgaaaa
     2341 ccgggaccac ggtgcggacc gctcgaaaac cggttccaaa cagccgcaaa ggcaagcgca
     2401 ttctgtccac tcgcattccg aacctcccgc actgaccgca cagaaaggaa ttgaaacgtg
     2461 cggcggcggt tggagcaagt gccggctgaa ttatgccggc agccccaatt caggaggaaa
     2521 ttcggactgg gcacttcggg ttcggaagcg gcgtggcagg gcgaaggcca ccaagcacgc
     2581 gctcagccgc gttagccggt gggcaagccg gctacctgca gcgcacgctc caccttctgg
     2641 tccagttgca tgtcgcccgg catctcgccg aagtcgccac gcagggcccg cagcgcgctg
     2701 cgctcctttt cgatttcatc gcgcgtgcgc gtcccaaggc ggcgcaaggc gagcaccggc
     2761 ggagcccagc cgcgcacggc gtgcatcaac aggaacccgg atgctatgta cggtaagggc
     2821 cgcaagagcc ggaacgcgcg gcccagtatc agagagccaa gaataattcc ggcggcattg
     2881 gcctggaacc tgcggccgag atcccattcc tgatccaact gtcgtaatcg ctcctcgatc
     2941 cgttccgggt gccgggcgta gtgcagcacc tggcactcga tctgctgctg catctgctgt
     3001 tggatgcggg catccatctc gtcaccgaaa cgctgcatgc gcgatttagc gctttccagt
     3061 tgcgtgatca cgtaattact cctgacgggc taatcggcaa gccggattcg gcgcaggcag
     3121 cctcgtcagt cctaatttcc ggtgcgcccc tgaaagcgcg ctgaaattgg cgtgacggcg
     3181 cggttagtgc cccggctttg tttcggccgc ctgaaacttg acccgcagtt tctgctcacg
     3241 cccatcgcgc aagaccacga tgtctgtctc gtcacgctcg tcgaatttct cgagcacgcg
     3301 catgtaggca tagatgtcgc ggatctcctc gcccgcgatc ttcatcacgc gatcgccatt
     3361 ttgcataccc gcaagcgccg ccgggctgcc ttcgattacg gtttgcacta gcatgcccgg
     3421 tttgccgtcg cccggcacgt ccggaatcac gcccagccgc acacgcagct tcgcgcgcga
     3481 aggcttggag tcggagtctt gcgtgctgct cgcgtcctgc ccggcctcct tcttctcctc
     3541 gaccgccggc ctgatttcct cttcttcacg cgcctcgggc ggcggctccg tgtaagccgg
     3601 cccggattcc atgttggcca gttcggccag aacgccgtag catagtcgca gcaccttcgc
     3661 ggccccctca gcgtctatga gctgccagtc atcttcgggc cgatggtact ccttgtggac
     3721 gccggtaaac gcgaacagca cggggatacg ccgggctgcg aacggcgcgt gatcgctgcc
     3781 gccggccatc agaccgccgg tgcggcagtt cagcccaacc tgcccggcgt acttctcaat
     3841 cagccccgcg aactccgccg ccgtcggagc gcctaacatc gaaaactttc ccgagccggc
     3901 gcggccgatc atgtcgaggt tgatcatggc gcggatgtcg tcgaaagcga cagtagggtg
     3961 ctcgacaaac tcgcgactgc cgcacagacc cagttcctcg ccgtcaaacg cgatgaagag
     4021 cacgttgcgc cgcagctttg tagcctctga cgcggctcgg gccaactcga tcacacccgc
     4081 cgtgccggaa gcattgtcgt ctgcgccgtt gtgaatctgg ggcgtcgggt cgttgccgtg
     4141 cggctgtatg cccaggtggt cgtagtgcgc gcctaccacg atggtttcgc tcgtatcacc
     4201 ggcgccgggc aacaagccaa gcacatttct cgcgggcacg cggtttggtt ccagcccggt
     4261 ttcaagcttg acgttgagcc ccatgtcgcg cgacagcggc ttgcgttgcg tatccaagcc
     4321 ctgttgcagc gtggctagat ccggcagccc cgccttggcg agcagcgtgt tcgccatagc
     4381 gcgtgagatg ctgaccatcg
//
LOCUS       DAOSYS010000285         4382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16343_length_4382_cov_5.264849, whole genome shotgun sequence.
ACCESSION   DAOSYS010000285 DAOSYS010000000
VERSION     DAOSYS010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16343_length_4382_cov_5.264849"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PLU99_13900"
     CDS             <1..1061
                     /locus_tag="PLU99_13900"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO18184.1"
                     /translation="VLAAVKARAPHAQVEIHNVLDSAGRFFKKLYGGGYVNLIRYFPT
                     GMGWLYDYTDRPAGGFDDRLRVAAQNLFVARTARYIVSRAPQLIINTHFLAAEIVSEL
                     RRTRRLDCPQTTVTTDFETHRMWVNEPTERYFTATDLGRFYLTTWGPAPERVFTTGIP
                     VRPGFLKPLGKEEARAMLGLHQKLPCVLLLGGARGINAPDTLLRQLIEMDAEAQIAVV
                     GGRDEALRQRLQRLAASARRPVRVLPYTDRIYEWMYAADLAVGKPGGLTVAEVLVCGL
                     PLVIISPIPGQETRNSDYLLENWAAIKVSHPRLLGYRVSQLLNDPVALQRLQDSSRRL
                     GRPHAADDIARLSLDLIT"
     gene            1133..1885
                     /locus_tag="PLU99_13905"
     CDS             1133..1885
                     /locus_tag="PLU99_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO18185.1"
                     /translation="MRYLVAIPVFNEQRHVVRVLKAVAQYCGDILVIDDGSSDSTPAL
                     LARHPNVAVIRHPENRGYGQSLIDAFHYASRLDYDWVITMDCDEQHEPEQIPEFLDRI
                     QTDTADVISGSRYLRPFPDDDPAPPERRLINFTINRILTQVLGVELTDSFCGFKAHRV
                     SAMRRLTLDEPGYAFPLQFWVQCALAGLRICEIPVRCIYPDRSRSFGGTLDDPAARLQ
                     HYLEVFVRELRREAVALPRETGLGLYCCTPRR"
     gene            1882..3444
                     /locus_tag="PLU99_13910"
     CDS             1882..3444
                     /locus_tag="PLU99_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18186.1"
                     /translation="MISYDRLVAPRGHLGVLIEPAPAEIRALLGEVQPRLEPINILGR
                     PLSAWRTDLRSQLELNGPVIATGHQAEFFHAGVFAKIIAAHVLSRHTHGSAVFVAVDS
                     DVGKTRRLEVPQITSRSLRRVDVTIPGLGTLRPDECQPEVPREQWVDFFFRVASVYEH
                     YDVSMLRPFSQGWLADGEQIRYCDGLARGFRALEAALGLDGVRELRISELCGTPAFRA
                     FAAQLMLNAESSAAGYNAARQAFRERHGVDNPARPVPPLVTQDDRIEVPLWIMRCDGP
                     RARLFLRRSGEQLDLLADRTFVTSMDVTALADRAAHDQPWELERLGWRLRPRALTLSG
                     FVRLFLADLFIHGIGGAKYDEVTDDYLTRFLGAPPPPMCCVTATLYLPLPRTGVTEEQ
                     VAQARRRARDLIYNPQRYVRNPSPDLLRRRAELIRRSQELAAHDYNNHTERRAVFDGI
                     RSVNEHLLRQDSWRAAEFQQEIERLQHAREQDEIALNREYFVGLHLKHSLEELVSRIS
                     RALIPEALQARS"
     gene            complement(3542..3625)
                     /locus_tag="PLU99_13915"
     tRNA            complement(3542..3625)
                     /locus_tag="PLU99_13915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3589..3591),aa:Leu,seq:caa)
     gene            complement(3681..>4382)
                     /locus_tag="PLU99_13920"
     CDS             complement(3681..>4382)
                     /locus_tag="PLU99_13920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOO18187.1"
                     /translation="RARVLPPGATVRMRQTIDVGPPRRVLRGTPQHLQRVVIRALLDP
                     VQGRDGQWRPGLAGQSLRPVYLNRVPAQVGTAALRVLFGELASEVERDRFWAVELIAE
                     LLGEQQRASLGRLDYRPEPVPVERLQQALAAAMASESAELRARTLDALQAAGLDASLL
                     AGVQAGLSSPDWLVRLMAVRLLGERKPPQWEARLRPLAVEDADELVRALAASYLPALE
                     PPGGEIGSATGAPAH"
BASE COUNT          767 a         1501 c         1332 g          782 t
ORIGIN      
        1 gagtcttggc ggccgtaaaa gctcgcgcgc cccacgcaca ggtcgaaatc cacaacgtgc
       61 tcgattctgc cggccgcttc ttcaaaaagc tctatggcgg cggctacgtg aatctaatcc
      121 gatattttcc gacgggcatg ggctggttgt acgactacac cgatcgtccg gccggcggct
      181 ttgacgatcg cctgcgcgtc gcggcccaga acctctttgt cgcccgcact gcccgataca
      241 tcgtcagccg tgctccccaa ctcataataa atacccattt tctcgcggcc gaaatcgttt
      301 ccgagctgcg ccgcacgcgc cgcctcgatt gcccacagac aaccgtaaca accgatttcg
      361 aaacccatcg catgtgggtg aacgagccga ccgaacgata cttcacggct accgatctgg
      421 ggcgcttcta cctgaccacc tgggggccgg cccccgagcg cgtcttcacc accggcattc
      481 ccgtgcgccc gggcttcctg aagccgttag gcaaggaaga agcccgcgcc atgcttgggt
      541 tgcatcagaa gctaccttgc gtcctcctgc tgggcggcgc gcggggcatc aatgcaccgg
      601 acacgctgct ccggcaattg atcgaaatgg acgccgaggc gcaaattgct gtcgtaggcg
      661 gccgcgacga ggccctgcgg cagcgcctgc aacgcctggc agcctccgct agacgccctg
      721 tccgcgtgct gccatatacg gatcgaattt acgaatggat gtacgcggcc gacctcgccg
      781 tcggtaagcc aggcgggctg acggtggccg aggtcctcgt gtgcgggctc ccgctggtga
      841 tcatcagccc aattccggga caagagacgc gaaacagcga ttacctgctg gaaaactggg
      901 cggcgatcaa ggtaagccac ccgcggctgc tgggttatcg cgtgagtcag cttctgaacg
      961 accccgtcgc gctgcaacgt ttacaggact cctcgcggcg cctcgggcgc ccccacgcgg
     1021 cagatgatat cgcccggctt tccctcgact tgatcacgta ggcggggttt gactattcgc
     1081 cgagcggcga aacaatcgac tgctgacaca agaccggcac ggcggagccg aaatgcgcta
     1141 tttagtggct atcccagtgt tcaacgagca gcgccatgtc gtgcgcgttc tcaaggccgt
     1201 ggcccaatac tgcggcgaca ttttggtcat cgatgacggc tcctcggata gcacgccggc
     1261 gctgctggcg cgtcacccaa acgtggcggt aatcaggcac cccgagaatc gcggttacgg
     1321 ccaaagcctg atcgacgctt tccattacgc ctcgcgcctt gactacgact gggtgatcac
     1381 gatggattgc gacgagcagc acgaaccgga gcagattccc gagttcctcg atcgcatcca
     1441 gactgacacg gcggacgtaa tcagcggctc gcgctacttg cggcctttcc cggacgacga
     1501 cccggccccg cccgaacggc gcctcatcaa ctttacgatc aaccgcatcc tgacgcaggt
     1561 cttgggagtg gagctgacgg attcgttttg cggtttcaag gcgcatcgcg tgtccgccat
     1621 gcgacgcctg acgctcgacg agccgggcta tgccttccca ttgcagttct gggtgcaatg
     1681 cgcgctggcc ggcctgcgca tctgcgaaat ccccgtgcgg tgcatctatc ccgaccgcag
     1741 ccgcagcttc ggcggcacgc tggacgatcc ggccgcgcgg ctgcaacatt acctggaggt
     1801 tttcgtgcgt gaactgcggc gggaggcggt cgcgctgccg cgcgaaaccg gactgggtct
     1861 ctactgctgc acgccaaggc gatgatttca tacgaccggc tggtggcgcc acgcggacac
     1921 ttgggtgtgc ttatcgagcc ggccccggcg gaaatccgcg cgcttctcgg cgaggtacaa
     1981 ccgcggctgg agcctatcaa catccttggg cggccgctga gcgcctggcg gacggatctg
     2041 cgctcgcagt tggaactgaa cggcccggtg atcgcgaccg gccaccaggc cgaattcttc
     2101 catgcgggcg tctttgcgaa gatcatcgcg gcccatgtac taagccgcca cacacacggt
     2161 tcggcggttt tcgtggcggt tgattcggat gtcggcaaga cacggcgcct ggaagtcccg
     2221 cagatcactt cgcgatcatt gcggcgtgtc gacgtgacca tccccgggct cggcaccctt
     2281 cgccccgacg agtgccagcc ggaggtaccg cgcgagcaat gggtcgattt cttctttcgg
     2341 gtggcctccg tttacgagca ctacgatgtt tccatgctga ggccgttctc ccaggggtgg
     2401 ctggcggacg gcgaacagat ccgctactgc gacggcctgg cgcgcggctt tcgggcgctg
     2461 gaggcggccc tcggcttgga cggcgtgcgc gagctgcgga tatccgagct gtgcggaacg
     2521 ccggcgtttc gagctttcgc cgcgcaactc atgctcaacg ccgaatcatc ggcggccggc
     2581 tataacgcag cgcgccaagc cttccgcgag cggcacggcg tagacaatcc cgcccggccc
     2641 gtgccgcccc tggtgacgca ggatgatcga atcgaggtcc cactgtggat catgcgttgc
     2701 gacggaccgc gggcgcgcct cttcctgagg cgctcgggtg agcagctaga cctgctcgcg
     2761 gaccgcacgt ttgttacgag catggacgtt acggctctgg ccgatcgcgc ggcgcacgac
     2821 caaccgtggg agttagagcg cctcggctgg cgcctgcgcc cgcgggcgct gaccttgtcc
     2881 ggcttcgtac gcctgttttt ggcggatttg ttcattcatg gcatcggcgg agcgaagtac
     2941 gacgaggtta cggacgacta cttgacccga tttctaggcg ctccgccgcc gccgatgtgc
     3001 tgcgtgacgg ccactctata tctgccgttg ccgcggaccg gcgtgaccga ggaacaggtg
     3061 gcgcaggccc gtcggcgtgc gcgcgacttg atttacaatc cccagcgcta cgtgcgcaat
     3121 ccgtccccgg acctgttgcg gcgccgggcc gagctaattc gccgctcgca agaattggcg
     3181 gcccacgact acaacaatca caccgagcgt cgcgcggtgt ttgatggaat tcgctccgtc
     3241 aatgagcatc tgctccggca ggattcctgg cgcgcggccg aattccagca ggaaatcgaa
     3301 cggctgcaac atgctcgcga gcaggacgag atcgcactga accgcgagta cttcgttggg
     3361 ctacacctca agcacagcct ggaggaactg gtcagccgta tctcacgcgc actcatcccc
     3421 gaagcgctcc aagcacgcag ctaatccgtg tggtaacgtg ataaggtgat agagtgataa
     3481 aggacggtct gcgcgcagcc tttatcacct tatcacctta tcactcggat tcccccgtca
     3541 ctgccgaagg agggactcga accctcacga ccttgcggcc acgggatttt gaatcccgcg
     3601 cgtctgccaa ttccgccact tcggccgagc ccgcgtgctc tcacgcggac agcgtatttt
     3661 tgtgtctgga gcgccgaata tcaatgggcc ggcgcgcccg tcgcggagcc aatttctcca
     3721 cccggcggct cgagcgccgg gagatacgag gccgccagcg cccgtaccag ctcatcggcg
     3781 tcctcaacag ccagcggacg caaacgtgct tcccactgcg gcggcttgcg ctcgcccagc
     3841 agccgcaccg ccatgagccg gaccagccag tccgggctcg acaggccggc ctgcacgcct
     3901 gccagtaaag atgcgtcgag cccggccgct tgcagagcat ccagcgtccg tgcccgcagc
     3961 tccgccgact cgctcgccat cgccgccgcc aaggcctgct ggaggcgctc gaccggtacc
     4021 ggttcaggcc ggtaatccag ccgtcccaga ctcgcccgct gctgttcgcc cagcaactcg
     4081 gctatcagct ccaccgccca gaaccggtcc cgctcgacct cgctggccaa ctcgccgaac
     4141 agcacccgca gcgccgccgt gcccacctgc gccggaacgc gattcagata caccggccgt
     4201 agcgactgcc ccgccagccc cggccgccat tgcccatcac gcccctgaac cggatcgagc
     4261 agcgcccgaa tcacgacccg ctgcaagtgc tgcggtgtgc cacgcaacac gcgccgcggc
     4321 gggcccacgt ctatcgtctg acgcatccgc acggtcgccc ccggcggaag cacccgcgcc
     4381 cg
//
LOCUS       DAOSYS010000286         4357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16445_length_4357_cov_4.582985, whole genome shotgun sequence.
ACCESSION   DAOSYS010000286 DAOSYS010000000
VERSION     DAOSYS010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16445_length_4357_cov_4.582985"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /locus_tag="PLU99_13925"
     CDS             complement(<1..1911)
                     /locus_tag="PLU99_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOO18188.1"
                     /translation="MSDFLIQFNATLTDGVVSDVLRHREHMESLCVEHFAYPWGRVAI
                     QHGRGLGYQPLFSNERVYFAVGRPRIVDVTHEDDGSQGFARRWADWVDGAAIAQHYDD
                     LTGLFVLGCCGPEGVLLLTDRLGYYSAFVGRDKNGSVRAIGTLPELVARAAGRISDPD
                     LASVGEMLVRCTPGYPHTMWRGVRELAPGALHEFRAARDAPEHTSRSLWVPSEPQRYP
                     SLKQLRAQLTEALQHAAKDITRGAQRVAVALSGGVDSRTVLALVPPEKRAGAITFVDY
                     QNRECELARRVARLARVPHLTLVREPEFYARLPAREVALLGNMRTAMHAHGFAIADAG
                     LADRFDLVLGGLCADTLLKGWFWPDSVRALMAGRCGVPAFLRARLWPPRYSCVDPIRP
                     GGLWALRPEIQLEIQERLGARVAELRKIRPETAAEWTNFYPTCGDVANYPLANMRLFL
                     ADELFLHRDIIEVSRVAHHLPKLMQLLTRPVFTQLCGPLARIQTAKTGLPADLGWLNT
                     RLRLRLRRWRERFVKRPAAAPAPNHVLPWYTDDSWPDFRLLQKHSPAWATLRAHAAAS
                     EPGLDLLASVLHIDPRSVLCAFNETLSCEFQAAAIQIALWFADTDAGRELHEETRVRR
                     ASVMVSSLAAALT"
     gene            2293..3360
                     /locus_tag="PLU99_13930"
     CDS             2293..3360
                     /locus_tag="PLU99_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOO18189.1"
                     /translation="MRYAVIMAGGAGTRLWPLSRRDRPKQILTLFDSQSLLQIAYRRL
                     EQLFQPANTYVVTSARYSEQICAALPTLPRENLIAEPTGRDTANAIGLAAHLLALRDP
                     QGTMAVFTADHLITPQEEFVRAVRAGLEAAEQFPESLVTFGIAPTGPHTGYGYIQRGP
                     AVRPALYRVAAFKEKPTREVAEQYVSSGEFYWNSGMFVWRLPTILRELDRRLPENSRV
                     LGELAREWSKLSNDVLTDKFGKLERISIDYAVMEHASDVLVVEMRCEWLDVGSWTAVG
                     RTRKADQAGNVTIARRSLAVDATNNILISEDEHLVAALGVRDLVIVHAPDVTLVCHRD
                     HAEAVRELVALARQRYGDAYD"
     gene            4151..>4357
                     /locus_tag="PLU99_13935"
     CDS             4151..>4357
                     /locus_tag="PLU99_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014444916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18190.1"
                     /translation="MLVYPGSPYPLGAVFDGNGTNFSLFSEAAERVQVCFFDDDDNET
                     AVDLPEQTAFCWHGYFPTISPGQRY"
BASE COUNT          846 a         1344 c         1370 g          797 t
ORIGIN      
        1 agtcaacgcc gcggcgagac tggagaccat tacgctggcg cgccgcacgc gggtttcttc
       61 gtgcagctcg cggccggcat ctgtgtcggc gaaccacagc gcgatctgga tggctgccgc
      121 ctggaattcg caactgagcg tctcattgaa cgcgcagagg acgctgcgcg ggtcgatgtg
      181 cagaactgag gcgagcaggt ccaatccggg ttcgctcgcg gcagcgtggg cgcgcagcgt
      241 ggcccaggcc ggggaatgct tctgcaggag gcggaaatcg ggccaggagt cgtccgtgta
      301 ccaaggcaag acgtggtttg gcgccggtgc ggccgcgggg cgtttgacga agcgctctct
      361 ccagcggcgc agccgcaagc gcagacgcgt gttcagccac cccaggtcgg cgggcagccc
      421 ggtcttggcg gtctggatgc gtgccagcgg cccgcacagt tgcgtgaaga ccggcctcgt
      481 aaggagctgc atcagcttcg gcaggtgatg cgcgacccgg ctgacttcaa ttatgtcgcg
      541 gtgcaggaac agctcgtccg cgaggaacag gcgcatgttc gccaagggat agttcgccac
      601 gtcgccgcac gtcgggtaaa agttcgtcca ttcagccgcg gtttcgggcc ggatttttcg
      661 cagttccgcg acgcgtgcgc ccaagcgttc ctgaatttcg agctggattt cggggcgcag
      721 ggcccacagt ccgccggggc ggatcgggtc tacgcaactg tagcgtggcg gccaaaggcg
      781 ggcgcgcagg aacgccggca cgccgcagcg cccggccatc agcgcgcgaa cgctgtcggg
      841 ccagaaccag cctttcaaga gggtgtcggc acacaagccg ccgaggacca agtcgaaccg
      901 gtcagccagc ccggcgtcgg caatggcaaa accatgggcg tgcattgccg tccgcatgtt
      961 gcccagcaag gccacttcgc gtgcgggcaa gcgggcgtag aattccggct cgcgcaccag
     1021 cgtcaggtgc gggacgcgtg ccaggcgtgc aacgcgccgc gccaattcgc actcccgatt
     1081 ctggtaatca acaaaggtta tggcgccggc ccgcttctca ggcggcacga gcgccagaac
     1141 cgtacgtgaa tccacaccac cgctaagtgc gaccgctacc cgctgcgcgc cgcgggttat
     1201 gtccttggcg gcgtgttgca gagcttctgt taattgcgcg cgaagctgct taagcgaagg
     1261 gtagcgctgc ggctccgagg gcacccagag actgcgcgat gtatgttccg gcgcatcacg
     1321 ggcagcgcgg aactcgtgca gggcgccggg cgccaactcc ctgactcccc gccacatagt
     1381 atgcggatag ccgggcgtgc agcgcaccag catctcaccc actgaggcca aatccgggtc
     1441 cgaaatcctc cccgccgcgc gcgccaccag ttccggcaat gtcccgattg cgcgcacgct
     1501 gccgttcttg tcgcggccaa caaacgccga gtagtaaccc aggcggtccg ttagcagcag
     1561 aacgccttca ggaccacaac agcccagcac aaaaaggccc gtcaagtcgt cgtagtgctg
     1621 cgcaattgcg gcgccgtcga cccagtccgc ccatcggcgt gcgaaaccct gcgaaccgtc
     1681 atcttcatgc gtaacgtcca caatccgcgg gcggccaacc gcaaaataga cgcgctcgtt
     1741 cgaaaaaagg ggctgatatc caaggccgcg gccgtgctga atcgctacac gaccccacgg
     1801 ataggcaaag tgctctacgc acaaactttc catgtgctcg cggtggcgca ggacatcaga
     1861 cactacaccg tcagtaagcg ttgcgttgaa ttgaatgaga aagtcactca taaaccacag
     1921 gctcgtgagt acacaatcag ccgtctgtca gtaagtgcac tgcccgttac gccaacaact
     1981 agacaatggt gcgcttcttc ccaaccgcga tccttcggcc cgtcgcaatt ctagtcgcgc
     2041 accacacgcc cggctcaatc tcggccgggg cttgctcgac tttttgaacg cgtagatatg
     2101 acgcgtcaga caggcccgtg acgcttcgac gacgacacag atgaagtcgc tgcgatcaga
     2161 acagcgctgg acgatgcttg cgaaatttca acaacccgga gatattggcc ccggcgggac
     2221 ggataccaaa cgtgtaccgc ccgtgacgac gggtcggccg cgcggccaat ccaccgtaca
     2281 ggagcaggcg ttatgcgtta tgcagtaatc atggccggcg gcgccggcac gcgactctgg
     2341 ccgctgagcc ggcgtgaccg cccgaagcag atattgacgc tctttgacag ccagtcgctg
     2401 cttcaaatcg cttatcgccg gctcgaacag ctatttcaac cggcaaatac atacgtggtt
     2461 acgtccgcac gctacagcga gcagatttgc gctgcgctgc ccaccctccc gcgcgagaac
     2521 ctgattgccg agccaaccgg gcgcgacacg gccaacgcga tcggcctggc ggcacacctg
     2581 ctggcactgc gcgacccgca aggcaccatg gcggttttca cggccgatca cctgataacg
     2641 ccgcaggagg aattcgtgcg agccgtccgg gccggattgg aggcggcgga gcaattcccc
     2701 gaaagcctgg tcaccttcgg catcgcacca accggtccgc acaccgggta cggctatatc
     2761 cagcgcggcc ctgcggttcg gcctgcgctt taccgagtgg ccgcgtttaa agagaagcct
     2821 acgcgcgagg tagcagagca gtacgtcagc tcgggcgagt tttactggaa cagcgggatg
     2881 ttcgtgtggc ggttgccgac catacttcgc gagttggacc gacggctgcc ggagaactcg
     2941 cgcgtgttag gcgagctcgc gcgcgagtgg agcaagctgt cgaacgacgt gctgaccgac
     3001 aagtttggca agctggagcg gatttcgatc gactatgccg tgatggagca cgcgtcagac
     3061 gtgctggttg ttgaaatgcg gtgtgagtgg ctggatgtcg ggtcttggac ggcggtgggc
     3121 cgaacgcgga aggcggatca ggcgggcaac gtaacgatcg cgcggcgttc gctagcggtc
     3181 gatgcgacaa acaacattct cattagcgag gatgaacacc ttgtggcggc gctcggagtc
     3241 cgcgacctcg tcattgtgca cgccccggac gtgactttgg tttgccaccg agatcacgct
     3301 gaagccgtcc gggagctggt cgcgctggcc cggcagcgct acggcgatgc gtacgactaa
     3361 gctccgtggt ttctgctatt cggcctatta gcggcggcga ttagccctaa ttagtgctcg
     3421 tgtggcgttt tgtatacatc acgtgtttcc gaatcgcaac aactagcgtt aaatcatgca
     3481 gggacaagat attgcgcgcc ggtcactgcg ctcaaatgag cgatcaagcc gcgatccacg
     3541 ttgaaggatt acaacgagtc cctacgtccc atatcatgtg gtccgcaaaa aaaacgcgca
     3601 cggagaagcc ggtttatttc ggacttttta agcataccca cgagagtgcc caactgcgcc
     3661 ttaacttatt ggcacggacg ttgcagcggg agcgcttttg cggtcccgcg aggtgctttc
     3721 ccgagggaaa agcgagggtt catgcatcga cgggtccgag aggaacggtg ttttagttct
     3781 catcactagc tgctgagatg gcgtaccggg atcgtttttt ggccgccgga ggttcgcgta
     3841 ataagttccg gtaaaaagca ccccttttcg gattccgtca aacgagcggc ccaggccaac
     3901 tggtttgggc cgtttgcttg cgccgccggc ggccctcaat agttatcgcc ccctcccccc
     3961 gccggggcct gtggttggca ctaatcggcc ttcatttcct tttcagccct tcgtcaggac
     4021 cgtcggctat cgactcctat catctagccg cacaggacgc gccgaggatg ccggctggcg
     4081 aaggcgtgcc gtgtttcggg aggcgcacgg acggcgtctc gacagtaccg caaaacgcga
     4141 atgggtgcct atgctggtct atcctgggtc gccgtatccg ctgggggcag tcttcgacgg
     4201 caacggtacc aacttctcgt tgttctccga ggccgccgaa cgggtgcaag tgtgcttctt
     4261 cgacgacgac gataacgaga ccgcggtgga tctgccggag cagacggcgt tctgttggca
     4321 cgggtacttc ccgacgatca gcccgggcca gcgttac
//
LOCUS       DAOSYS010000287         4351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16474_length_4351_cov_5.723929, whole genome shotgun sequence.
ACCESSION   DAOSYS010000287 DAOSYS010000000
VERSION     DAOSYS010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16474_length_4351_cov_5.723929"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /gene="aceE"
                     /locus_tag="PLU99_13940"
     CDS             complement(<1..2040)
                     /gene="aceE"
                     /locus_tag="PLU99_13940"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOO18191.1"
                     /translation="MPPRENVPDSDPLETAEWLASAEAVKRHFGTQRVEFLLEQLREW
                     CYRSGARFPFSANTPYINTIPVAEQPPFPGDREIERRIKNYARWNAMAMVIRANRAAD
                     GIGGHLSTYASATTLFEIGFNHFFHAKTDRSAGDQVYFQGHAAPGIYARAYLEGRIDE
                     THLVNFRRELAKGGGLASYPHPWLMSDFWEFPTVSMGLAAINSIYQARFNYYLQDREL
                     ADTAQSRVWGFFGDGEMDEPESLGAITLAGREKLDNLIWVINCNLQRLDGPVRGNGNI
                     IQELEGAFRGAGWNVIKVIWGGDWDPLFAADHEGVLIDLMEDTVDGQWQRYAVESGAY
                     CREHFFGRDPRALRLVDHLTDDQIRTLRLGGHDPEKVYAAYAAAVAHRGQPTVVLART
                     IKGYGLGEAGEGRNIAHQQKKLNEQELRYFRDRFEIPVTDKQLLEAPFCRFAPDSPEY
                     EYLIERRRALGGFIPRRVVRAEPLTAPSLRLFTEFLKGSPRPAATTMAFMRMLARLLS
                     DSELGRLIVPIVPDEARTFGMDALFRNYGIYARFGQLYEPVDAHLLMRYEERKNGQIL
                     EEGITEAGSMASFTAAGTAYATHRVNAIPFFIYYSMFGFQRIGDLIWAAADARARGFL
                     VGGTAGRTTLAGEGLQHQDGHSQLLFSVVPNCVSYDPAFAYELAVIIQDGIRRMYQ"
     gene            2274..2588
                     /gene="rplU"
                     /locus_tag="PLU99_13945"
     CDS             2274..2588
                     /gene="rplU"
                     /locus_tag="PLU99_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOO18192.1"
                     /translation="MYAIFEDGGKQYKVSTGDKLLIELKELPEDQTQVVFDKVLMTGE
                     GAEARIGTPWLEGASVAATVIEETKLPKVTGVKFRRRKGYLKKWGHRQRALLVRIDAI
                     NA"
     gene            2731..3465
                     /locus_tag="PLU99_13950"
     CDS             2731..3465
                     /locus_tag="PLU99_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18193.1"
                     /translation="MLLFCVLLAGEGAAAPGDFAQKKGNRPPSGQIRRGGKKKPRRSK
                     PKKPAQKPDKDESESEEAATSQPAEPEDGPETVATVLRRLGRMRRGEMSERDIALKAG
                     LEFVRALCRADGEKASALLEVTGYQVIPGDGLGDEPGPRITPEAFAAALAAGRAAAFE
                     TLPAECVEAAPPEALREKFPGISTWMLPTDWGIVLKPVAERHDWVQREACLVVRLRAR
                     KPTVQGGTALEPWPGILRAGSGEIGS"
     gene            complement(3515..>4351)
                     /locus_tag="PLU99_13955"
     CDS             complement(3515..>4351)
                     /locus_tag="PLU99_13955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOO18194.1"
                     /translation="LALEVLPVFSHKGPLSELARQAARAVIEETLALAEPEWQDMARA
                     IRADCAEAAVFFDVDLGGGLRVYDLYADAQEEAGRAALAVQLAARDAHRLRRAMTDRL
                     TGVANRACFELRMAELLRGLSPRRFALLTIEVDRFAQLGSDLGLAAADRVLRQVARRL
                     RTSLSDVDLLARWGGPRFVVVAPRGDRHGACVAAVRACRAVERSSIQLDSQPLQITVS
                     VGLVYSSDYSVPVDLETLTNDGQRQLQLSKDAGGNTWSYLGRTASQAVAATQAAAQAP
                     LP"
BASE COUNT          748 a         1270 c         1583 g          750 t
ORIGIN      
        1 ctggtacatc cgccggatgc cgtcctggat gatgaccgcc agctcgtagg cgaaggccgg
       61 atcgtacgag acgcagttcg gcacgacgct gaagagaagc tggctgtggc cgtcctggtg
      121 ctgcagccct tcgccggcga gcgtggtgcg gccggcggtg ccgccgacca ggaagccgcg
      181 ggcgcgggcg tcggcggcgg cccagatcag gtcgccgatg cgctggaagc cgaacatcga
      241 gtaataaatg aagaacggga tcgcgttgac acggtgcgtg gcgtaggccg tgccggccgc
      301 ggtgaaggag gccatcgagc cggcctcagt aatgccttcc tcgagaatct ggccgttctt
      361 gcgttcctcg tagcgcatga gcaggtgtgc gtcgacgggt tcgtagagct ggccgaagcg
      421 ggcgtagatg ccgtagttgc ggaacagggc gtccatgccg aaggtccggg cttcgtcggg
      481 gacgatcggc acgatgagcc ggccgagttc gctgtcggac aagaggcgcg ccagcatgcg
      541 catgaaagcc atggtggtgg cggcgggccg cgggctgcct ttgagaaact ccgtgaacag
      601 gcggagcgaa ggagcggtca gcggctcggc gcggaccacg cggcgtggga tgaagccacc
      661 cagggcgcgg cgacgttcga tcaggtactc atactcagga ctgtcgggcg cgaagcggca
      721 gaacggcgcc tcgaggagtt gcttgtcggt gaccgggatt tcaaagcgat cgcggaagta
      781 acgcagctcc tgttcgttca gcttcttctg ctgatgggcg atgttgcggc cttcgccggc
      841 ctcgcccagt ccgtagccct tgatcgtgcg ggccagaacg acggtgggct ggccgcgatg
      901 cgcgacggcc gcggcgtaag cggcgtagac tttttccggg tcatgcccgc ccaggcgcag
      961 cgtgcgaatc tggtcgtcgg tgagatggtc aacgagtctc agggcacgcg ggtcgcgccc
     1021 aaagaagtgc tcgcggcagt aggcgcccga ctcgacggcg tagcgctgcc actggccgtc
     1081 gacggtgtcc tccatgaggt caatcaggac gccttcgtgg tccgcggcga agagcgggtc
     1141 ccaatcgccg ccccagatga ctttgatgac gttccagccg gcgccgcgga aggcgccctc
     1201 caactcctgg atgatgttgc cgttgccgcg gacggggccg tcgaggcgct ggagattgca
     1261 gttgatgacc cagatcaggt tgtcgagctt ttcgcgcccg gcgagggtga tagcgccgag
     1321 gctttccggc tcgtccattt cgccgtcgcc aaagaagccc caaacgcggg actgggcggt
     1381 gtcggccagt tcgcggtcct ggaggtagta gttgaagcgg gcctggtaga tagagttgat
     1441 cgccgccagc cccatcgaga cggtagggaa ctcccagaaa tcagacatca gccatgggtg
     1501 cgggtatgac gccagcccgc cacctttggc caattcgcgg cggaaattga ccaggtgcgt
     1561 ttcgtcgatg cggccttcga gataggcgcg agcgtagatg ccgggagcgg cgtggccctg
     1621 aaaatagacc tggtcgccgg cggagcggtc ggtcttggcg tggaagaagt ggttaaagcc
     1681 gatctcgaag agcgtcgtgg ccgaagcgta cgtcgaaagg tggccgccga tgccgtccgc
     1741 ggcccggtta gcccggatga ccatggccat ggcgttccag cgggcgtagt tcttgatgcg
     1801 gcgttcgatc tcgcggtcgc cggggaaagg cggctgttcg gcgaccggga tagtgttgat
     1861 gtagggcgtg ttggccgaga atgggaagcg ggccccgctg cggtagcacc actcgcggag
     1921 ctgctcgagc aggaactcca cgcgttgcgt gccgaagtgg cgtttgacgg cttcggcgga
     1981 ggccagccat tcggcggttt cgagcgggtc ggagtcgggt acattttcac gtggcggcat
     2041 aggcgtcttc agctaattat ggggttcttg tgagccgtcg gtcggcccag gtcaaacggc
     2101 aacggcatgc ccgggccgct ccgcgggctt gggcatggca acctcgcacg ggcatggcac
     2161 ccgggctgcg tatctcgacc cgcgcctgcc ggcttcgctg atgccggttg cattcggcgt
     2221 cgctacggct taaactgctt gattctagca ttaaccggca atgaggtacg agcgtgtacg
     2281 cgatatttga ggacggcggt aagcaataca aggtcagcac cggcgacaag ctgctgattg
     2341 agctgaagga attgcccgag gatcaaacac aggtggtgtt cgacaaggtc ctgatgacgg
     2401 gcgagggggc cgaggcgcgc atcggcacgc cctggctgga aggcgccagc gtcgccgcta
     2461 cggtgatcga agagaccaag ttgccgaaag tcaccggcgt aaagttccgg cggcgcaagg
     2521 gttacctcaa gaagtggggt caccggcagc gggccttgtt ggtgcggatc gacgctatca
     2581 atgcgtgata gccatgcggc gcaggcttga gggcctgtgg aagtggtgcg cgcgccgtct
     2641 agtcttccaa ggcgggcctg cgggctcgtg aaagatgcgg ggcgggcttg tcggttgtca
     2701 gcgggtcgcc acccgatccg gctggcaata atgcttttgt tctgcgtttt actggccggc
     2761 gaaggggcgg ccgctccggg ggatttcgcc cagaagaaag gcaatcgtcc gccgtccggt
     2821 caaatccgca ggggcggcaa aaagaaaccc cgccggagta agcccaagaa gccggcccag
     2881 aaacctgaca aagacgaatc tgaatctgaa gaggcggcga ccagccagcc agcggaaccg
     2941 gaggacgggc cggagacggt tgcgacggta ctgcgccgtc tggggcgcat gcggcgtggc
     3001 gaaatgtcag agcgggacat cgctttgaag gccgggctgg agttcgttcg tgccctgtgc
     3061 cgtgcggatg gcgagaaggc ttcagcgttg ctggaagtga cgggttacca ggtcattcca
     3121 ggcgatggac tcggtgacga gccggggccg cgcatcacgc cggaggcatt cgccgccgcg
     3181 ctggctgcgg gtagagccgc ggcgttcgag acgttgcccg cggaatgcgt tgaagcggcg
     3241 ccgcctgaag cgttgcggga gaagttcccc gggatttcga cctggatgct gccgacggat
     3301 tgggggattg ttctgaaacc ggtcgccgag cgtcacgatt gggtgcagcg tgaagcctgc
     3361 ctggtggttc gcttgcgcgc gaggaagccg accgttcaag gcggcactgc gcttgagccg
     3421 tggccgggca ttctgcgggc gggatcgggc gaaattggtt cctgactaac ttagggggct
     3481 cgctggtact tcgggctcga atcgggtggg cacattaagg cagtggggct tgggccgcgg
     3541 cctgggtcgc tgctactgct tgggaagcgg tccggcccag gtaggaccag gtgttcccgc
     3601 cggcgtcttt ggacaattga agttggcgct ggccgtcgtt cgtgagcgtt tcgaggtcga
     3661 ctggcacgct gtaatcagaa ctgtagacca ggccaacgct gaccgtgatt tgcaacggct
     3721 ggctgtcgag ctggatgctg gagcgctcga ccgcccggca ggcgcggact gccgcgacgc
     3781 aggcgccgtg ccgatcgccg cgcggcgcca ctaccacaaa acgcgggccg ccccagcgcg
     3841 ccaacagatc aacgtcgctc agcgaggttc gcaatcgccg cgcgacttgc cgcagcactc
     3901 tgtctgccgc cgccaatccg aggtcactgc cgagctgcgc gaaacggtcg acctcaatag
     3961 tgagcagggc gaagcgccgg ggcgacaggc ccctgagtag ttcagccatc cgcagttcga
     4021 agcaggcccg gttggcgacg cccgtcagcc ggtcggtcat ggcacgccgc agccggtggg
     4081 cgtcgcgggc cgccaactgc accgccaggg cggctcgccc ggcctcctcc tgggcgtcgg
     4141 cgtagaggtc gtagacccgc aagccgcccc cgaggtcgac gtcgaagaaa accgcggcct
     4201 cggcgcaatc ggcgcgaatg gcgcgggcca tgtcctgcca ctcgggctcg gccagggcca
     4261 acgtctcctc gattacggct cgtgccgcct gccgggctag ctcgctcaag gggcctttgt
     4321 gggagaatac cggcaacact tccagcgcca g
//
LOCUS       DAOSYS010000288         4256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_16896_length_4256_cov_4.573197, whole genome shotgun sequence.
ACCESSION   DAOSYS010000288 DAOSYS010000000
VERSION     DAOSYS010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16896_length_4256_cov_4.573197"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /gene="nadD"
                     /locus_tag="PLU99_13960"
     CDS             complement(<1..464)
                     /gene="nadD"
                     /locus_tag="PLU99_13960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOO18195.1"
                     /translation="MPRILLFGGSFDPIHHGHLIISRFVAEHLAAERVLLIPAAKPPH
                     KPGRDLTPGEHRLAMCGLAVEGEPLFEVSDWELTQPGLNYTLNTVRHFAATLPRGTEL
                     FWLIGMDSLSELATWYHVSELVDACTIVTAARPGFEAGDLPDLARVLTHSQM"
     gene            complement(466..1158)
                     /locus_tag="PLU99_13965"
     CDS             complement(466..1158)
                     /locus_tag="PLU99_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18196.1"
                     /translation="MPDQAGSGPIREPARAAASGPDPAILERLLRAQSPADVLAEVLG
                     SPPAEVQRAAVVALGWKGVAAHGSLLRGLLHHPETGLADLAEDSLWRLWLRAGSARSN
                     ADLARAIDLIQSNRCHEALAALDSLLAAEPAFAEAHHQRGIALFMLDRPADAALAFKR
                     AVELNPDHYPAMVSLGHAALHQGELRSALLCYRRALELNPRLTTVREMAQKLELMVGG
                     NGNMLTDRSVGL"
     gene            complement(1406..2362)
                     /locus_tag="PLU99_13970"
     CDS             complement(1406..2362)
                     /locus_tag="PLU99_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO18197.1"
                     /translation="MVPGLCKVEPDLKPPAPAVMDSLSPADAKLVRRRLREPVECVFD
                     PCFLEPGSEEALLAPIERAPGTDGAANDDSGADLLATGRPVCLTAEQEQQMFLRLNYC
                     RYRVVQIVREYAGRRLPPEKLAELLRWEHLAAQTREDIIRVNIPLVLAMAKRTRITGV
                     DFSDLISEGNLALLRSVEKFDCARGYKFSTYACRAILKSFSRVAMRTSRYRGYFPTEF
                     DPSLEKSDFVEKKRTDAEGDCLDELKSILVRNLASLSEVEQQVIRARFALDQACGQVQ
                     AARAKTLEQVGEMIGVTKERVRQIQNKALDKLRNVLEGGLLA"
     gene            complement(2824..3528)
                     /locus_tag="PLU99_13975"
     CDS             complement(2824..3528)
                     /locus_tag="PLU99_13975"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOO18198.1"
                     /translation="MAIFTETWSGKFRGHDVRQRRIELLNRQYTFIGPCAPEALLDAP
                     NAEARFAADGYMPYWAEFWPASLLLMDAVASWAPPGPGAPPVKVLELGCGLGAVSVVL
                     GSLGYRVFASDYDGDALAFTMENARINGVTTIEPLLIDWREAYPGLRVDRIVAAEVLY
                     ECRNAEPVAQFVSRHLSPTGFALICDRNRSTADDFPEVARRFGMQVEVSAVQRGDPDG
                     GRPISGRLFRLELREL"
     gene            3637..4188
                     /locus_tag="PLU99_13980"
     CDS             3637..4188
                     /locus_tag="PLU99_13980"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO18199.1"
                     /translation="MALKPGRASARPKPYRLEQFDPSQAALVASWVCDAHEAYWLAPK
                     TAPPVTAADVRAWNRPGHEQLQLVPARGRLPVAYGELNLLDQQRGLYWLGHLLVAPQR
                     RRCGVGAALTSALVERAFSHYAAAAVTLVVFPDNAAARACYYRAGLREDGWEVHYLPP
                     YGRRVRLLRMSIKRPAQLAVPSF"
BASE COUNT          768 a         1419 c         1306 g          763 t
ORIGIN      
        1 tccatctggg aatgtgttag cacgcgcgcc agatcaggca ggtcgcccgc ttcaaacccc
       61 ggacgggccg ccgtcacgat cgtgcacgca tcgaccagtt cgctgacatg ataccaggtg
      121 gccagctcgc tcaggctatc catgccgatg agccaaaaga gttcggtgcc ccgcggcaag
      181 gtcgctgcaa agtgccgcac agtgttgagc gtgtagttca ggccgggctg cgtcagctcc
      241 cagtcgctca cctcgaagag cggctcacct tcaaccgcca gaccgcacat tgccagccgg
      301 tgctcgccag gcgtcaggtc gcgccccggc ttgtggggcg gtttggcggc cgggatcagt
      361 aggacccgct ctgccgcgag gtgctcggcc acaaaacgac tgataattag gtgcccgtga
      421 tgaatggggt cgaaggagcc gccgaaaagc aggatgcgtg gcacatcaaa gccccacgga
      481 ccggtcggtg agcatgttgc cgttgccgcc gaccatgagt tcaagcttct gggccatttc
      541 gcgtactgta gtcagccgcg ggttcaactc caaggcccgc cggtagcaaa gcagcgcgct
      601 gcgcagctcg ccctggtgca gggccgcatg cccaaggctc accatggccg ggtaatgatc
      661 cgggttgagt tcgaccgcgc gtttgaacgc cagcgccgcg tcggccgggc ggtccagcat
      721 gaacagggcg atgccgcgct gatgatgcgc ttcagcaaag gccggttcag ccgcgagcag
      781 tgaatccagc gccgccaaag cttcgtggca ccggttagac tggatcagat cgatcgcccg
      841 cgccaggtcc gcattgctgc gggccgagcc cgcgcgcagc cagaggcgcc aaaggctatc
      901 ttcggccagg tcggctagac ccgtttccgg atgatgaagc aggccccgca gcagcgagcc
      961 atgtgcggcg acacccttcc agcccagcgc gacgactgcg gcccgttgca cctcagccgg
     1021 cgggctgccg agtacctcgg ccagcacgtc ggccggcgac tgcgcacgca gcaggcgctc
     1081 caagattgcc gggtccggac cgctagcagc cgctcgagcg ggctctctta tcggaccgct
     1141 gcctgcttga tctggcacaa cgctctccgc cagccactga atgcggtcta caaagcaacc
     1201 gctcagcgaa ttaccaggga acgagactct ggctgcgggc cagttccggc aacaacggag
     1261 ttgcattcaa tcctaggaag cgggtacgga caggtcaaac gtttttcagg acgtgcgata
     1321 ccccccgcga gccgccgcgc cgagtcgccc gcaagtaccc gggcgacccg ccgcgacgaa
     1381 gatgttgttg cgccgccagc ccgaactacg cgagcaggcc cccctccagc acgtttcgca
     1441 gcttgtccag cgccttgttc tgtatctgcc ggacccgctc tttggtcacg ccgatcatct
     1501 cgcccacttg ctcgagcgtc ttggcgcgcg ccgcttgcac ttgaccgcag gcctggtcca
     1561 gggcgaaacg ggcgcggata acctgttgct cgacctcgct gagcgaggcc agattgcgaa
     1621 cgaggatcga cttcagctcg tccaggcaat cgccttccgc gtcggtgcgc ttcttctcga
     1681 cgaagtcgct cttctccaac gagggatcga actcggtcgg gaagtacccg cgatagcgcg
     1741 acgtccgcat cgccacccgc gaaaagctct tgaggatcgc gcggcaggcg taagtgctga
     1801 atttgtaacc ccgcgcgcaa tcaaattttt cgacgcttcg cagcagggcc agattgccct
     1861 cgctgatcag gtccgagaag tccacgcccg tgatccgcgt ccgcttggcc attgccaata
     1921 ccagcggaat gttcacgcgg atgatgtctt cccgagtctg cgccgccagg tgttcccaac
     1981 gtagcagctc ggccagcttc tccggcggca gtcggcgccc ggcatattcg cgcactatct
     2041 gcaccacacg gtagcgacag tagttcagac gcaggaacat ctgctgttcc tgctcggccg
     2101 tcagacacac ggggcggccg gtggccagca gatctgctcc gctgtcgtcg ttggcagcgc
     2161 cgtcggtgcc tggggccctc tcgatggggg ctaatagtgc ttcttcgctt cccggctcca
     2221 gaaagcacgg atcgaacacg cattccacgg gttcccgcag gcggcgccgc accagcttcg
     2281 cgtctgctgg tgacaagcta tccatgaccg ccggcgcggg cggcttcagg tcaggctcca
     2341 ccttgcacag acccgggacc atactgcatt ccctggcaag aaccactgcg ttgctccctt
     2401 cttctcctcc accgcctgtg gacaaaccag cagacggcga agtgcgcaag cacttagcac
     2461 gagcgacaac gcgcgtgcgc gccacaacta ataaccagaa gccgtattcc cgaccttggg
     2521 gagccttcag acgcagtcgg gcgccggcaa ctatggggag cctttgcaca aaaagtataa
     2581 cttatgtttc gcgccgctgc aaggtatcgt tcatcgacct gcaagctggg gtcgatgcga
     2641 ggtgatactg accaggcgta aaacgtgctg agttcgtaag ttacaatcgc gtcaaggcgc
     2701 tcgcggccgc gggggtcctg gtgcgaagga cagttagatt attcttgcgt aaggtggatc
     2761 caatgtaatg gtcgctggcg aagccgcgcc ggtttatcgg ccgctgccgc gttgcggctc
     2821 gtctcataac tcgcgcaatt cgagcctgaa aagccttccg ctgatcggcc gtcctccgtc
     2881 agggtcaccg cgctggactg cggaaacctc tacttgcatg ccgaaccgcc gggctacttc
     2941 cggaaagtcg tcagcggtcg aacgattacg atcgcatatc aaggcaaagc cagtagggct
     3001 aagatgccgt gaaacaaact gcgcgaccgg ttctgcgttc cggcactcat ataggacctc
     3061 cgccgcaaca atgcggtcga ctcggagccc cgggtaagct tctcgccaat cgatgagaag
     3121 aggctcgatc gttgtcacgc catttattcg ggcgttctcc atcgtgaacg ccagggcatc
     3181 cccgtcataa tccgaggcga acacccgata accaagactt ccgagaacga cgcttaccgc
     3241 gccgaggccg cacccgagtt cgagcacctt caccggcggc gcgcccgggc caggcggcgc
     3301 ccagctcgcg acagcgtcca tcagcagcag cgaggccggc cagaactccg cccagtatgg
     3361 catgtagccg tcggcggcga agcgcgcctc ggcgttgggc gcatcgagca gggcctccgg
     3421 cgcacatggt cctataaacg tgtactgccg attcaacagc tcaatgcgcc gttgccgcac
     3481 gtcgtgcccg cgaaacttcc ctgaccatgt ttccgtgaaa atcgccatca gtcctgtatc
     3541 attactgcct gtctcgtagc cggtcgcgtt cttccgcccg ggccgtgccg gcaagctcga
     3601 cccggcggtg gccatacagg ttacacggag tgcagggtgg ccctcaaacc cgggcgcgca
     3661 agtgctcggc caaagcctta tcgcctcgag cagttcgacc cgtcacaggc ggccctggta
     3721 gcgtcctggg tgtgcgacgc gcacgaggcg tactggttgg ccccgaaaac cgcgccgccc
     3781 gtcacggccg ccgacgtgcg cgcctggaac cggcccggtc acgagcagct ccaactcgtg
     3841 ccggctcgcg gtcgcctgcc cgtcgcctat ggagagctga acctactgga ccagcagcgc
     3901 ggcctgtact ggctgggaca tctgctggtc gccccgcagc gacggcggtg cggcgtggga
     3961 gcggcgctca ccagcgctct agtagagcgt gccttttcgc actacgcggc cgcggccgtc
     4021 acgctcgtgg tttttcccga taatgccgcg gcccgcgcct gttattaccg ggctggcctg
     4081 cgcgaggatg gctgggaagt gcactactta ccgccctacg ggcggcgcgt gcggctgctg
     4141 cgcatgtcca taaagcgccc ggcgcagctt gccgtcccaa gtttctagtt attgtaggat
     4201 acccgcgcgg cgccggcggc tcgccggcgt gcgttccgag cggtgttcta gtcatt
//
LOCUS       DAOSYS010000289         4205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17154_length_4205_cov_4.260964, whole genome shotgun sequence.
ACCESSION   DAOSYS010000289 DAOSYS010000000
VERSION     DAOSYS010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17154_length_4205_cov_4.260964"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2516)
                     /gene="clpB"
                     /locus_tag="PLU99_13985"
     CDS             complement(<1..2516)
                     /gene="clpB"
                     /locus_tag="PLU99_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOO18200.1"
                     /translation="MNLERLTIKAAEAVQRAQQMAQNAGNPELTPVHLLAALVGAGSP
                     AVGAPEEDGGIVVPVLEKAGAHVSQIRSMAEAELRRLPQTRGGSLSLSRQLAEVFQAA
                     EREASRMKDQYISTEHLLLALADVASDAKEILRLNAAGKDDILAALKEVRGTAAVTTP
                     SPEDTYEPLKRYGRDLVEAARQGKLDPVIGRDEEIRRCMQVLARRTKNNPVLIGEAGV
                     GKTAIVEGLAQRILAGDVPQALKDKKVVALDMGALIAGAKYRGEFEERLKAVIKAVVE
                     SNGQVILFIDELHTVIGAGRAEGAVDAGNLLKPALARGELRCIGATTLDEYRQHVEKD
                     KALERRFQPVYVGEPTLEDTIAILRGLKPRYEAHHGVRIQDAALVAAATLSHRYITER
                     HLPDKAIDLVDEAASRLRIENDSLPAELDETRRRIMQLEIEREALKKERDESGRKQLE
                     NVERSLAELREQDSRLTAKWQNEKSVIDAIKSLKEQIDAKQTELQDAQRRGELERAAR
                     IRYGELPELERQLRDREKRLTELHAQGDGMLREEVTPDEIAEIVSKWTGVPVARMLEG
                     EREKLIKMEERLHARVVGQDEAVRAVSDAVRRARAGLGDPNRPIGSFLFLGPTGVGKT
                     ELCKALAEFLFDDEAAFVRIDMSEFMERHSVARLIGAPPGYVGFEEGGRLTEAVHRRP
                     YSVILFDEIEKAHPDVFNVLLQVLDDGRLTDGHGRTVDFRNTLIAMTSNIGSQYIAEL
                     ATAREGGEASAPVEIEIEMRVKEELKKYFRPEFLNRIDEVIIFHRLSKKELRGIVDLQ
                     VRYLQRRLADRDMTLRLTDAAKDRLASDGYDPVYGARPLKR"
     gene            complement(2806..3726)
                     /locus_tag="PLU99_13990"
     CDS             complement(2806..3726)
                     /locus_tag="PLU99_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18201.1"
                     /translation="MLLQRAKGFRWAFAASMAAVLIGSSFSSAGVIYFSDAYYPTTSD
                     GFIRSVNTDGSGLQTILSIGGGVRGMDVAGNRIFWSDVDNDVIQRSYLDGSGVETVVG
                     SGLSFPMGLATDAAHNRLYWGDALLGRIDSSDMDGGGAAPLLTTPFGNGLELYGGKLY
                     WTTAVTGDSGDIMRANLDGSDPEVVLTGEGRPSDIALFGGKVYWTDYVLDRVRRANLD
                     GTGVEDLYIVGANWNPGGITIDPVEQKVYWAQSTGVGYESALMRMNLDGSEQETLLSG
                     IGIGVSLCWLPEPTALALLAVGALALRRRS"
     gene            4019..>4205
                     /locus_tag="PLU99_13995"
     CDS             4019..>4205
                     /locus_tag="PLU99_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18202.1"
                     /translation="MQKHRTLRALFARCAIPFLLCPVLITTATGHPYSPRTNTSDTLL
                     FAGLGTYERPVNTSSPVA"
BASE COUNT          720 a         1588 c         1070 g          827 t
ORIGIN      
        1 cgcttcagcg ggcgagcccc ataaaccggg tcgtatccat cgctggccag ccggtctttc
       61 gccgcatccg tcagccgcag cgtcatgtcc ctatcggcca gccgccgctg tagataccgg
      121 acctgcaaat caacaatccc gcgcagctcc ttcttgctca ggcggtggaa gatgatcacc
      181 tcatcgatac ggttcaggaa ttccgggcgg aagtacttct tcagctcctc tttcacccgc
      241 atctcaattt cgatttctac cggcgcgctc gcctcgcccc cctcgcgggc cgtcgccagc
      301 tccgcaatgt actgactccc gatgttgctg gtcatcgcga tcagcgtgtt gcggaaatcc
      361 accgtccgcc catgcccgtc cgttagccgc ccatcgtcca gaacttgcag cagaacgttg
      421 aaaacgtccg gatgcgcttt ctcgatctcg tcgaacagaa tgacgctgta tggccgccgg
      481 tgaacggcct ccgtcagccg gccgccctcc tcgaacccta cgtagccggg cggggctccg
      541 atcaggcggg ccacgctgtg ccgttccatg aattcgctca tgtcgatccg cacgaacgcg
      601 gcttcgtcgt caaacaggaa ctccgccagc gccttgcaca actccgtctt gccgaccccg
      661 gtcgggccga ggaacaggaa cgatccgatc gggcgattcg ggtcgcccaa cccggcccgc
      721 gcacgccgca cggcatccga caccgcccga acggcctcat cctgcccaac gacgcgcgca
      781 tgcagccgtt cctccatctt gatcaatttc tcccgctcgc cttcgagcat ccgggcaacg
      841 ggcacgccgg tccacttgct cacaatctcc gcgatctcgt cgggcgtcac ttcctcgcgc
      901 aacattccgt ccccctgcgc gtgcaattcg gtcagccgct tttcccggtc gcgcaactgc
      961 cgctccaact cgggcaattc gccataacgg atgcgcgcgg cgcgctccag ctccccgcgg
     1021 cgttgggcat cctgcagctc cgtctgcttg gcgtctattt gctccttgag cgacttgatc
     1081 gcgtcgataa cgctcttttc gttttgccac ttggcggtca gccgcgaatc ctgctcgcgc
     1141 aactccgcca ggctgcgctc gacgttctcg agctgtttcc gcccggactc gtcgcgctcc
     1201 ttcttcagcg cctcgcgctc gatctcgagt tgcatgattc gccggcgcgt ctcatccaac
     1261 tccgccggca gcgaatcatt ctcaattcgc agccgggagg ccgcctcgtc caccaggtcg
     1321 atggccttat ccggcaagtg ccgctccgtg atgtaccggt gcgacagcgt cgccgcggcg
     1381 accagcgcgg catcttgaat gcgcacgccg tgatgggcct cgtaccgcgg cttcagcccg
     1441 cgcagaatcg cgatcgtgtc ctccagggtc ggctcgccca cgtacaccgg ctggaagcgc
     1501 cgctctaacg ccttgtcctt ttcaacgtgc tgccggtatt cgtcgagcgt cgtcgccccg
     1561 atgcaccgca gctcgccccg cgccagcgcc ggcttcagca aattaccggc atccaccgcc
     1621 ccctccgctc ggccagcccc aatcaccgtg tgcagctcgt cgatgaacag gatgacctgc
     1681 ccattcgatt cgaccaccgc ctttatgacg gcctttaatc gctcctcgaa ttccccgcgg
     1741 tatttcgcac ccgcaatcag cgcccccata tccagggcca cgaccttttt gtcctttaga
     1801 gcctgcggta cgtctcccgc cagtatccgt tgcgccagcc cctcgacgat ggccgttttc
     1861 cccacccccg cctcgccgat cagcaccggg ttgttcttcg tccgccgcgc caacacctgc
     1921 atgcagcggc gaatttcctc gtcccggccg atcactggat cgagcttccc ctgccgcgcc
     1981 gcctccacca ggtcccgccc gtaacgcttc agcggttcgt aggtatcctc cggacttggc
     2041 gtcgtgaccg cggccgtccc gcgcacctcc ttcagcgccg ccagaatgtc gtccttgccg
     2101 gccgcattca gccgcagaat ctccttggcg tcactcgcca cgtccgccaa ggccagcagc
     2161 aaatgctcgg tcgagatgta ttggtccttc attcgcgaag cttcgcgctc cgccgcttgg
     2221 aagacctcag ccaactgccg gctgagcgac agcgagccgc cccgcgtctg cggcagccgc
     2281 cgcaactccg cctcggccat gctgcgaatc tgggaaacat gcgccccggc cttttccagg
     2341 acggggacca caatcccacc gtcttcctcg ggcgccccca ctgccggact gccggccccg
     2401 accagcgccg ccagcaagtg caccggcgtc agctccgggt tgccggcgtt ctgcgccatc
     2461 tgctgcgccc gctgaaccgc ctccgccgcc ttgattgtca atcgttccag gttcatgcct
     2521 tagctccgac ctacgtggga ccggtttcaa accagtcagg tcctgccttc ctcccctgcc
     2581 gagcatacgg cattccggaa cgagaattga cctaaatcct tgttatttgt gtacttaggg
     2641 tctatgaccg cgcccccgcg cgtgtcatta tggcagcgaa tgtgtgctca tgccccgttt
     2701 caaaaatgca gggcgtacgc agattgccga aaaaacacgc atctgcgtac gcccacgttt
     2761 acccggggcg atccccggat ggcgccgtta attcttgcct tgaacctaac tgcgtcgccg
     2821 cagcgccaac gcgcccaccg cgagcagcgc cagcgccgtc ggctccggta accagcacag
     2881 gctgaccccg atcccgatac cgctcagcag cgtttcctgt tccgagccgt ccaggttcat
     2941 ccgcattagc gccgactcat accctacgcc ggtcgactgc gcccagtaga ccttctgctc
     3001 caccgggtcg atcgtgatcc cgcccggatt ccagttcgcc cccacgatgt acaaatcctc
     3061 aacgccggtg ccgtccaggt ttgcccgccg cacccggtcg agcacgtaat cagtccagta
     3121 cactttcccg ccgaacagag cgatatcgct cggcctgccc tctccggtca gtacaacctc
     3181 cgggtccgac ccgtccaggt tggcgcgcat gatgtcgccc gagtcgcccg tcacggcggt
     3241 cgtccagtac agcttccctc catacagctc caatccgttt ccgaatggcg tcgtcagcaa
     3301 cggcgcggcc ccgcccccat ccatgtcgct gctgtcgatc cgacccagga gcgcgtcgcc
     3361 ccagtacagg cgattgtgag ccgcgtcagt ggccaggccc atggggaacg acagcccgga
     3421 gccgaccacg gtttcgacgc cgctgccatc caggtatgac ctctgaatta catcgttgtc
     3481 cacgtcgctc cagaagattc tgttgccggc cacatccatc ccgcgcacgc ctccgccgat
     3541 gctcaagatg gtctgcaacc cgctgccatc cgtatttact gaccggatga aaccgtcgct
     3601 cgtggttggg tagtacgcgt ctgagaagta aattacaccg gcgctggaaa atgagctgcc
     3661 aatcagtaca gccgccatgc tcgcagcgaa tgcccagcga aaacctttcg ccctctgcaa
     3721 caacatcctt cctctcctat gcaaaaaaag tgccttctcg caattgaacg acaattgcac
     3781 tcgtccagta taccgcgcca cccgcatagt tcaaaaaatc ttcagcccag cgccggggtg
     3841 ccgcgtgtgg cattcggcta gcccgcaacc tccaagccgc caaccagcgc cagcactaat
     3901 cactaacaac taacaacctt cttcagtcac ccgcccgcgc cacatctaca atcgcgctac
     3961 caggaaagca tgggtatacg cccgtgaaaa acgtccgccc ccgccggaga tttgagtaat
     4021 gcaaaaacac cgcactctgc gcgccctctt cgcacgctgc gccattcctt tcctcttgtg
     4081 ccccgtcttg atcaccacgg ccaccggcca cccctactcg ccccggacga acacgtcgga
     4141 cacgctgctg ttcgccggcc tcggtacgta cgaacgcccg gtgaacactt cgtcacccgt
     4201 cgccc
//
LOCUS       DAOSYS010000290         4204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17158_length_4204_cov_5.641118, whole genome shotgun sequence.
ACCESSION   DAOSYS010000290 DAOSYS010000000
VERSION     DAOSYS010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17158_length_4204_cov_5.641118"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLU99_14000"
     CDS             <1..1239
                     /locus_tag="PLU99_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOO18203.1"
                     /translation="TTHWNHPGFMAYFAVTGSGPGVLGEALAAALNNNAMLWRTGPAP
                     TELEERVCDWLRQMMGLPPEFKGHINDTASISSMLALTAARQRVRDFDVRRRGLAGRS
                     ELPRLRIYASEQAHSSIAKAAIVIGVGLENLRKIPTDKEFRMQPAALAAAIREDRAAG
                     MCPMAVVATVGTTSTTSIDPVPEIAAICRREGLWLHIDSAYAGVAAMCPELRALMPGL
                     GEADSLVTNAHKWLFVPSDCSVLFVRDAAQWRETFSIVPHYLSTSETDATNLMDYGVQ
                     LGRRFRSLKMWMVLRAFGVSGLQERIRKHCRLARMFADWVRAEPGFELIAPVLFSTIC
                     FRAVPDLPPAQQDEFNARLLQTVNAAGPVLVSHTQLNDRYVMRIAIGSLRTEERHLAA
                     AWKLIRDTHREMLSSGYGRR"
     gene            complement(1240..2763)
                     /locus_tag="PLU99_14005"
     CDS             complement(1240..2763)
                     /locus_tag="PLU99_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO18204.1"
                     /translation="MRPVIRKPGNVVRPEDVDGVASFTSVVKARDNSYPHDPPGRTHR
                     ILVVDEDAIVLSALERMLRDSGYGVASVARLSEALHALEKLEFDLILADLRVETEDGC
                     QVLQTWRQRWPGTGVILIPRYGSIEAAVKAVRLGALDYLPKPLRAEHLRGVVQTALQQ
                     REHARRARSLTRPYDQPCTLQSVIGSTHQMQRVFDLIEAVAESTATVLIHGETGTGKS
                     LIARVIHSLSSRRDGPFVEVSCGAIPETLLASELFGHTRGAFTGAVADKDGKFRAAEG
                     GTILLDEISCAPPSLQMKLLRVLQERQFEPLGSNRTLTADVRVILATNVSLQKEVEAG
                     RFRADLFYRINVVNIELPPLRERLGDIPLLATHFLDHYAAKSHKRLTGFSDEALATMQ
                     RYYWPGNVRELANCIERAVVLTRHEQVLKSDLPPALVQAVDGDTPLHLAAGFTGSAMT
                     LKEALAEPERRILLAALRANNWNRIQTAEMLNINRTTLYKKMKRYRLDEAEPQRREA"
     gene            complement(2839..3411)
                     /locus_tag="PLU99_14010"
     CDS             complement(2839..3411)
                     /locus_tag="PLU99_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18205.1"
                     /translation="MAPLLARNALVLAASAALIGLTACAPAALGPRRIVSLAELESSP
                     PRAPAGLLCRAVDEPIALQPLIHPLGRRLWFLEVTNVEQWRQLAQAVPEVGPCPDFRR
                     GTVIGVVSRGTRLDGAAPFWLQTIRVHNGAGLVELSFNPGTYLADGSAYLEMTYVTGL
                     RNVLVVNVDGFDYVASGSARKDTGSRIPIR"
     gene            3531..>4204
                     /locus_tag="PLU99_14015"
     CDS             3531..>4204
                     /locus_tag="PLU99_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOO18206.1"
                     /translation="MIIGVPTEIKPQEYRVAVVPAGVEQLRRSGHTVLVQKAAGVNSG
                     FADEDYQALGADIVETADEIWQQADLICKVKEPLPQEIKKIRSGQVLFTYFHFAANRE
                     LTEGVRDSNCVAIAYETVVDREGRLPLLAPMSEVAGKMSIQEGAKYLEKPMLGRGILL
                     GGIPGVEPANVLVLGGGIVGTCAARVAAGLGANVVMMDIDLYRLRYLAEVMPANVRTV
                     YSTPAA"
BASE COUNT          767 a         1302 c         1378 g          757 t
ORIGIN      
        1 accacgcact ggaaccaccc gggcttcatg gcctacttcg ccgtaaccgg ttccggcccc
       61 ggcgtccttg gcgaggcgct ggcggccgcg ctcaacaaca acgccatgct atggcgcacc
      121 gggcccgccc cgaccgagct ggaagagcgc gtatgcgact ggctgcggca gatgatgggc
      181 ctgccaccgg agttcaaagg gcacatcaac gacaccgcgt ccatcagctc gatgctggcg
      241 ctgaccgccg cccgccagcg ggtacgcgac ttcgacgtcc gccgccgcgg cctggccggg
      301 cgctccgaac tgccgcggct gcgaatctac gccagcgagc aggcgcactc gtccatcgcc
      361 aaggcggcca tcgtcatcgg cgtcggcctg gagaatttgc gcaagatccc gaccgacaaa
      421 gagtttcgca tgcagccggc cgcgctggcc gcggcgatca gagaagaccg ggcggcgggg
      481 atgtgcccga tggcagtggt ggcgacggtc ggcacgacgt ccaccaccag catcgacccg
      541 gtgccggaga tagccgcgat ttgccggcgt gagggcttat ggctgcatat cgactcggcg
      601 tatgcgggcg tggcggccat gtgcccggag ttgcgggcgc tgatgccggg cctgggcgag
      661 gccgactcgc tggtcaccaa cgcacacaag tggctgttcg tgccgtcgga ttgctcggtg
      721 ctattcgtgc gtgatgcggc gcagtggcgc gagacgttct cgatcgtgcc gcattacctg
      781 agcacgagcg agacggatgc gacgaatctc atggattacg gcgttcagct tgggcggcgc
      841 ttccgttcgc tgaagatgtg gatggtgctg cgggcgttcg gcgtctccgg cttgcaggag
      901 cgcattcgca agcactgccg cctggcgcgg atgtttgccg actgggtgag agcggagccc
      961 ggtttcgagc tgatagcgcc ggtgctgttc agcacgatct gtttccgggc cgtgcccgat
     1021 ttgccgccgg cgcaacagga cgagttcaac gcccggctgt tgcaaaccgt caatgcggcg
     1081 gggccggtac tggtctcgca cacgcagctc aacgaccggt acgtgatgcg aatcgcgatc
     1141 ggcagcctgc ggaccgagga gcgccacctt gccgcggcct ggaagctgat ccgcgacacg
     1201 caccgtgaga tgctctcgtc gggctatggc cgtcgctgat tatgcctcgc gccgctgggg
     1261 ttcggcttcg tcgaggcggt agcgcttcat cttcttgtac agcgtcgtac ggttgatgtt
     1321 gagcatctcc gccgtctgaa tgcggttcca gttgttggcc cgtagggcgg ccagcaatat
     1381 ccggcgttcc ggttcggcca gcgcttcctt gagggtcatg gcagatccgg tgaatccggc
     1441 cgccaggtgc agcggcgtat cgccgtcgac cgcctgcacg agggccggcg gcaggtcgga
     1501 tttgagcact tgctcgtgcc gcgtcaggac gacggcgcgt tcgatgcagt tggccaattc
     1561 acgaacgttg ccgggccagt aatagcgctg catcgtggcc agggcttcgt ccgaaaagcc
     1621 ggtcagccgc ttgtgcgact tggccgcgta atggtcgaga aagtgggtcg cgagcaatgg
     1681 gatgtcgccc aggcgctcgc gtaagggcgg cagctcgatg ttgaccacgt taatgcggta
     1741 gaaaaggtcc gcgcggaacc gcccggcctc cacctctttt tggagcgaga cgttcgtggc
     1801 cagaatcacg cgcacatcgg ccgtcagcgt gcggttggac ccgagcggtt cgaactgccg
     1861 ctcttgcagc acgcgcagca gtttcatttg caggctcggc ggtgcgcatg aaatctcgtc
     1921 cagcagaatg gtgccgccct cggccgcacg gaatttgccg tccttgtccg cgactgcccc
     1981 ggtgaatgca ccgcgcgtgt ggccaaacag ctcagaagcc agcaaggtct ccgggattgc
     2041 gccgcagctt acctcgacga atggtccatc gcgtcgcgaa ctgagcgagt gaattacgcg
     2101 cgcgatcagt gatttgcccg tgcccgtttc gccgtgaatg agtaccgtgg ccgtcgattc
     2161 cgcgactgct tcgatcaggt caaacacacg ctgcatctgg tgcgtgctgc cgatcacgct
     2221 ttgcagggtg cagggctggt cgtacgggcg cgtgagactg cgcgcccgac gcgcatgttc
     2281 acgctgctgc aatgcagtct gcacgacgcc gcgcagatgc tccgctcgca gtggtttggg
     2341 caggtaatca agcgctccca gccgcacggc cttgaccgcc gcttcgatgc tgccgtaccg
     2401 cgggatcagg atgactcccg tgccaggcca gcgctgccgc caggtttgca gaacctggca
     2461 accgtcttcg gtctcgaccc gcagatcggc gagaatcaaa tcgaactcta acttttcgag
     2521 cgcgtggagc gcttcagata gccgggcgac ggatgcgacg ccgtatccgc tatcccgcaa
     2581 catccgctca agcgcgctca atacaatcgc gtcctcatcc accaccagga tgcgatgagt
     2641 gcgaccgggg ggatcatgcg gataagagtt gtcgcgcgcc ttgacgacgg acgtgaatga
     2701 cgccactccg tctacgtcct ccggcctgac gacgtttccg ggtttcctga taaccggacg
     2761 catcacgaag gggctgctcg aagactaaac cagggatcct gtttcgcaaa aacaacataa
     2821 gcgatcgggg gggcagtttc aacggatggg gatccgcgaa cccgtgtcct tgcgagcgga
     2881 accgcttgcg acatagtcaa aaccatccac gttcacaacc aggacatttc gcagcccggt
     2941 tacgtaagtc atttcaagat acgcacttcc atccgccaga tacgtccctg gattgaagct
     3001 caactcgact aagccggccc cattgtggac ccggattgtc tgcaaccaga acggggccgc
     3061 gccgtccaga cgtgtacctc tactgaccac gccaataacg gtgccgcgcc tgaaatccgg
     3121 acaaggcccg acctccggaa ccgcttgtgc aagttgtcgc cactgctcga cgttcgtcac
     3181 ttccagaaac cacaagcggc gacccagagg atgtatcaaa ggctgcagcg cgatcggttc
     3241 atccaccgcg cgacaaagga gtccggccgg cgcgcgtggc ggcgaggact caagctccgc
     3301 gagacttacg atgcgtcggg gcccgagcgc tgccggcgcg caggcggtca gtccgatcaa
     3361 agccgcactc gcagccagca ccagtgcgtt gcgagcaagc agcggcgcca agctggcact
     3421 cagcttgcag tagggccgtg gcaagcttag tctcactggc cccagccggc gcccaggggc
     3481 aatccggcgg gtatatcgac taaagcgccc cgactcagga gttttccggc atgatcatcg
     3541 gcgtcccgac agagataaaa ccgcaggagt accgagtggc agtggtgccg gcgggcgttg
     3601 agcaactgcg tcgctccggg cacaccgtcc tggtccaaaa ggcagccggg gtcaattcgg
     3661 gatttgcgga cgaagattat caggctcttg gcgccgatat tgtggagacg gccgatgaaa
     3721 tctggcagca ggcagatttg atttgcaagg tcaaagagcc gctgccgcaa gagatcaaga
     3781 aaatccgctc ggggcaggta cttttcacat attttcactt cgcggccaat cgggagttga
     3841 ctgaaggcgt gcgcgattcc aactgcgtgg cgatcgcgta tgagacggtc gtggaccgcg
     3901 aagggcgcct gccgctgctc gcgcccatga gcgaagtggc cggcaagatg agcatccagg
     3961 aaggggccaa gtatctggaa aagccgatgc tggggcgcgg catcctgctg ggcggaattc
     4021 cgggggtcga gccggcgaac gtgcttgtgt tgggcggcgg cattgtcggg acgtgcgccg
     4081 cgcgggtcgc cgcgggacta ggcgcgaacg tcgtgatgat ggacattgac ctgtaccggt
     4141 tgcgctacct ggccgaggtc atgccggcca acgtgcgcac ggtctacagc acgccggcgg
     4201 cgat
//
LOCUS       DAOSYS010000291         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17312_length_4173_cov_5.176542, whole genome shotgun sequence.
ACCESSION   DAOSYS010000291 DAOSYS010000000
VERSION     DAOSYS010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17312_length_4173_cov_5.176542"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            618..1448
                     /locus_tag="PLU99_14020"
     CDS             618..1448
                     /locus_tag="PLU99_14020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOO18207.1"
                     /translation="MYLGALQIGGLVAIGLAAGVLGGLLGVGGGILMIPALTFIFADK
                     YGPNSFHVYNLAAISTSFVLAIPAAIRHTRAGAVIYPFLRSIVPLAVIGVVAGVLLSA
                     TMTGERTRILKQIFGGCLEFVVLVNLIQEWIAKRGEPYLFPSSPMPRRWGLIGLLVGL
                     PAGFIAGLLGVGGGIWAVPSQSLLLGIRVRNAIATSMVMIIPVALVAAASMALKLAQL
                     PHEPPLQYAAWWLTLCLAPGALLGGWCGASLMHRLPVRLVRYIFYALLSVTGLRLLFP
                     "
     gene            1479..1679
                     /gene="iscX"
                     /locus_tag="PLU99_14025"
     CDS             1479..1679
                     /gene="iscX"
                     /locus_tag="PLU99_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HOO18208.1"
                     /translation="MKWSDTEEIGIRLHEKFPDVDPLSVRFTDLLRWISELEDFGDDP
                     KAANEKKLEAVQMAWYEEFQGK"
     gene            1850..3064
                     /locus_tag="PLU99_14030"
     CDS             1850..3064
                     /locus_tag="PLU99_14030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOO18209.1"
                     /translation="MVRMTVLCLAAGMLAQVLPQGCLPEPTEEPVDTERRPILISAFA
                     PESAQCNETVTLTATAVGDLDGGEIAYSWLQISGPGVQILNANEAEATFVAPSLASDA
                     VLAFAVVTTNERGDFGRADVSVLIQADPDYGAQPEPGVAGAPVARAGPDREITEALPV
                     TLDGSNSRGDSLTYEWTQIPAATVELKGADTVKATFTAPAFVAGGINDLEFKLTVRDR
                     RGRSSHDTVLLKVMEGTGAEPKPHVRLTTSKGEIVLELDRTKAPISVDNFLRYVRDGF
                     YDGTIIHRVDSDVIQGGGFRPGLRPKEGTRDPIVNEADNGLKNLKYTIAMARTSDPDS
                     ATSQFYINLEDNPEYDARPGQVGYAVFGKVISGKDVVDAIGAVATEARGEFATVPIFD
                     VVVERAERIASD"
     gene            complement(3157..4083)
                     /gene="pyrF"
                     /locus_tag="PLU99_14035"
     CDS             complement(3157..4083)
                     /gene="pyrF"
                     /locus_tag="PLU99_14035"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOO18210.1"
                     /translation="MEHFADRLTAAVTARATPAIVGLDPVLERLPAELRRHANTASAA
                     AEATEEFCRAVLEAVAPVVPAVKINSAFFEVFHQAGIAAYYRLIAHAHSLGLLVIGDV
                     KRGDIGSTARLYAAAHLVEPPFSDIDPASIPDAVTLAGYLGESAVRPFIEAAKAGGRG
                     VFILVRPSDPGADEVHEFAGFGGSVRLYEMMGRLVERWGRRSDLIGHCGLSCVGAVVA
                     PKDLTSTATLRVQLPRTPFLVPGYGAQGASAAECRPCFRRDGTGAVVNASRSVIFAYE
                     RPDMIQQHGSDWRACIAAAARAFAADVAPLIQ"
BASE COUNT          735 a         1259 c         1394 g          785 t
ORIGIN      
        1 ttgcgaatgt attcgctggg cgcgtcggcg gcatgaccga ggaccacggc ttcggggcgg
       61 tgtttgtcgg tattgccgcc acgacaggcg ccgcggccct gctgctgttt gttctgtcgc
      121 cgctgctcaa gcgcatgatg cacggcatca agtagcagag ccggttcgtt cgccccggag
      181 cgttttcggt tcgaaaatcg cgagttcccg tttatcggcc actaaaatag tgcgattttt
      241 gttgccctgc tgcccccttc gacgtacaat gattgcgcag gccgagttac ggccagcgac
      301 ggccctcaaa agtgggcgag ggccgcaagc cgcggctcac ctgccgagcg acgcgtcagc
      361 gccgcgcgtt gggtcggtcg gcgacggccg gccgacaggg ctttggcggc ctgtacctcg
      421 ggtcgcctgg gtgcccaatt gaaaggaggt gatccgttgc caagtgataa acagaggtgg
      481 ctgcggtaac gcagtgaacg tggctgcata ccgcagcccc ccaaaattgg cccgccgacg
      541 accttagcag ttgccggcgg gttcttgtat ttaggggaga caatcaagcc cggtcgcggt
      601 taccatgccg gcccgttatg tacttgggcg ccctccaaat cggcggactc gttgcgatcg
      661 gcttggcggc cggcgttctc ggcggattgc tgggcgttgg cggcggaatt ttgatgattc
      721 cggcgctaac tttcattttc gccgataaat acgggccgaa ttcgtttcac gtttacaacc
      781 tcgccgcaat cagcacgtca tttgtgctgg ctattccggc cgccatccgc cacacgcggg
      841 ccggggccgt catttacccg ttcttgcgct ccatcgtgcc gttagctgtg ataggcgtcg
      901 tcgcgggcgt gctgctcagc gccaccatga ccggcgagcg cacgcggatt ctcaagcaga
      961 ttttcggcgg ctgtctggaa ttcgtggttt tggtcaatct catccaggaa tggatcgcca
     1021 aacgcggcga gccttacctc tttccgagca gcccgatgcc gcgtcgctgg ggcctgatcg
     1081 gcctgctggt gggcctgccg gccggcttca tcgccggcct gttaggcgtc gggggaggga
     1141 tctgggcggt gccgtcgcag tcacttctgc tggggattcg cgttcgcaac gccatcgcga
     1201 cgtcgatggt gatgatcatc ccggtggcgc tggtggcggc cgcgtcgatg gcgcttaagc
     1261 tggcgcagtt gccgcacgaa cccccgttgc agtatgccgc ctggtggctc acgctttgcc
     1321 tggcgccggg agcgctgctc ggcggctggt gcggggcgag cctgatgcac cgtttgccgg
     1381 tgcggctagt gcgatacatc ttctacgcac tactctcggt caccgggctg cgcctgcttt
     1441 ttccgtgata cccgaaggag cttcacgtga gcgccaaact gaaatggtcc gacactgaag
     1501 agataggcat ccgcctgcac gagaagtttc cggacgtgga cccgctgtcc gtccgtttca
     1561 cggacctgct gcgctggatc agcgagctcg aggatttcgg cgatgaccct aaggcggcca
     1621 atgagaagaa gctggaagcc gtccagatgg cttggtatga ggagtttcag ggcaagtaac
     1681 gtccttcgcc gcgacggcta cgccacgcgc gcggctcccg ggaacgttca gcgctgaggt
     1741 ttgggtcagc catggctaag actggactgg cagcatgtat aatggcttac ggtgatgtca
     1801 tttgcgcagc cgaggtcgag cgcctttcgg ccgcggagga gtttacgaaa tggttcgcat
     1861 gacggttttg tgtctggcgg cagggatgct ggctcaggtg ttgccgcagg gctgcctgcc
     1921 ggagccgacc gaggagccgg tggacacgga gcgtcgtccg atcctcatca gcgctttcgc
     1981 cccagaaagc gcgcaatgca atgaaaccgt cacactcacc gcgacggccg tcggcgacct
     2041 tgacggcggc gagatagcct atagctggtt acagatcagc gggccgggag tacagattct
     2101 caacgctaac gaggccgagg ccaccttcgt cgctccatca ctcgcttcgg atgccgtttt
     2161 ggccttcgcg gttgtgacta ccaacgagcg cggcgatttc gggcgggcgg acgtctcggt
     2221 gctcatccag gccgacccgg attacggcgc ccagccggaa ccgggcgtcg ccggcgcgcc
     2281 ggtggcccgc gcgggtccgg accgcgagat cacggaggcg ctgccggtca cgctggacgg
     2341 cagcaatagc cgcggcgatt cgctgacgta cgaatggacg cagattcccg cggccacagt
     2401 agaactaaag ggcgccgaca cggtaaaagc gacgtttacg gcgccggcat tcgtcgcggg
     2461 cgggatcaac gacctggaat tcaagctgac agtgcgcgac cgccggggcc gcagctcaca
     2521 tgacacggtt ttgctgaagg tgatggaagg tacgggggcc gagcccaagc cgcacgtgcg
     2581 tctgacgacc tcgaagggtg aaatcgtgct ggagctcgac cgcacgaagg ccccgatttc
     2641 ggtggacaat ttcctgcggt acgtaagaga tggcttttac gacggaacga tcatccaccg
     2701 cgtggactct gacgtgatac agggcggcgg cttccggccg ggactaaggc ccaaagaagg
     2761 aacgcgcgac ccgatcgtta acgaggctga taacggcctg aagaacctca aatataccat
     2821 tgccatggcc cgcacgagcg atccggattc ggcgacgtcg cagttctaca tcaaccttga
     2881 agataacccc gagtacgacg cgcgccccgg ccaagtcggc tacgccgtgt ttggcaaagt
     2941 catcagcggc aaggatgttg tagacgcgat tggggcagtc gcgacggaag cacgcggcga
     3001 gttcgcgacg gtcccgattt tcgatgtggt ggtggaaaga gcggagcgga tcgccagcga
     3061 ttagcgcgtc ggctgagtcc ttgcatcgct gctgacccgc ttaaaggctg ccagcgcgcg
     3121 cagcctgtgc tgaacacgct cgacaaccgg tgcagactac tgtattagcg gggcaacatc
     3181 ggccgcgaac gcccgggcgg ccgcggcgat gcaggccctc cagtcgctgc cgtgctgctg
     3241 gatcatatcg ggccgctcat acgcgaagat tactgaacgc gaagcgttga cgacggcgcc
     3301 tgtgccgtcg cggcggaagc atgggcggca ctcggctgcg ctggcgccct gcgcgccgta
     3361 gcccggaaca aggaacggcg tgcgcggcag ttgcacccgc aaggtcgccg tgctggtgag
     3421 gtccttcggc gcgacgaccg ccccgacgca cgacagaccg cagtggccga tcaaatcgct
     3481 gcggcggccc caacgctcga ccagccgccc catcatttcg tatagccgca ccgatccgcc
     3541 gaagcccgcg aattcgtgta cttcgtcggc cccaggatcc gaagggcgta ccaggatgaa
     3601 gacgccgcgg ccgccggctt tggcggcttc aatgaagggc cgcacagcgc tttcgcccag
     3661 atatccggcg agcgtgaccg cgtcgggtat gctggccgga tcgatgtcgg agaagggcgg
     3721 ctcgaccagg tgcgccgcgg catatagacg cgcggtggat ccgatgtcgc cgcgctttac
     3781 gtcgccaatg accagcaggc ccaggctgtg ggcgtgtgcg atcagccggt agtaggcggc
     3841 gattccggct tgatggaaga cttcaaagaa cgccgagttg attttgaccg cgggaacgac
     3901 cggggcgacc gcttcgagca ctgcgcggca gaactcttcg gttgcctcgg cggccgctga
     3961 ggcggtgttc gcatgtcgac gcaattcggc ggggaggcgt tccagaacgg ggtcaagccc
     4021 aacgatggcg ggcgtggcgc gggccgtgac tgcggccgtc aaccgatcag caaagtgttc
     4081 catagtgcgt catattggac agtggctcgt ggagtggcta gtggacgggt gcgggtcccg
     4141 ctattcactg cccacattca ctcaactcac cat
//
LOCUS       DAOSYS010000292         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17348_length_4167_cov_3.017753, whole genome shotgun sequence.
ACCESSION   DAOSYS010000292 DAOSYS010000000
VERSION     DAOSYS010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17348_length_4167_cov_3.017753"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLU99_14040"
     CDS             <1..735
                     /locus_tag="PLU99_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18211.1"
                     /translation="APWLSYNHARNIPGFAVSHAHLAAYHHAPEIPAVRKVLDWHGRS
                     RKSTSFGRGLDLARPGLDASWEEVRRFHEVIRARIAQQKSWQAETYRRKPRTMLLLIG
                     RIFGFQAGWIPRPEGVFNEDLSEEYTRKIRLREGGAEIAPWRIVVNDWLADWHPRAMG
                     CAYALAVLGAALSIGLRNRAGLLLAGVGLGNAALLAVLLLHSRRYTLPTEPFVILLGA
                     WALWRIFAAAKRRLGMGRDAPRRGDP"
     gene            843..1544
                     /locus_tag="PLU99_14045"
     CDS             843..1544
                     /locus_tag="PLU99_14045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO18212.1"
                     /translation="MDARYAAGRRHKPHLSFRLRCRARAAAGAYRRYRTHTQPPRVLD
                     FGAAEGATMVEVHRLLGAAESIGVEYAADLIAAAEPLPADCRLLQGDVTAPLPEITLG
                     HFDLVTALAVLEHLAEPAELIAQAHRALRPGGVFVATCPAGHWDRLSGRLRLHQDEHH
                     ETEFDRHRFAAVAEAGGLVPLRYERFMFAPLGFLPYLRIPVPVGLAEACDGCVRFLRV
                     FEFTFVNQLFVARRP"
     gene            1592..3232
                     /locus_tag="PLU99_14050"
     CDS             1592..3232
                     /locus_tag="PLU99_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18213.1"
                     /translation="MRNRYAELALPYIPRLLQLVDRNPYSPTYGCFDRSYWHYRTMDF
                     PCGMSQEFVLPLALAYANPYPGNVFHNVARVRELAEAGIRYMIPSSHANGTCDDYFPF
                     ERAMGALVFSLYAASEAYQILGMRDEQVVDLFLKRIKHLAAENETGQLSNHQALAALS
                     AYNVYLITGDDRCRRLAEDRVALTLSWQNAEEGWFQEYEGADPGYHTCTIDFLAKLQQ
                     KRGDTALLKPLLKAVDFAWHFMHPDGSYGGEYGSRNTYHFYPHGFELLAPHSEKAGQI
                     VDAFLRGTARDKRYHNDDDRMTAHYVYNFLQAWQDYHEHRPPPIAAGRSTPQTIWLPA
                     AKMAVSWNGREENSGGRYVVANLSKGGVLKVFDAAGPIASDTGLIGELTDGRVVVSHL
                     VQHDSTITADPARNEFSVTGWFCRRRRNLPTPFKFVIFRLLLLTIGRFNANLTRHLLQ
                     KILITGKPKTQFRFKRTLRLESDRVIVTDFVPRDMPLKRLSAGADATSIYVANSNVYQ
                     ESVLCRWRHVDPATLPVDGPWRVWRRAYYRGSGDALKD"
     gene            3237..>4167
                     /locus_tag="PLU99_14055"
     CDS             3237..>4167
                     /locus_tag="PLU99_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO18214.1"
                     /translation="MHTGPGCLEAAEKQDMARVTDLRAPSLPGDAPMIMIAPKHELTP
                     GLSVVVPCYNEEDAVCQTVEQVAQVLAGMNCPTEMIFVDDGSTDRTPALLDELAGRHP
                     RLRVVHNHANCGYGASLKRGISAARYDRIIITDADGTYPNERIPDLYHALDHADMVVG
                     ARIGPRVHIPLARRPVKWLLLKYSRWMAKADIQDINSGLRGIWTRHVRAFWSLLPNAF
                     SFTTTITLATHINQLRVEYLPIDYYQRVGKSSIRPLQDTFRFFTLVFRTIMYFRPLQI
                     FGTAALILMFAAGLAAVGVKLWTGLVPDVTVMSL"
BASE COUNT          743 a         1519 c         1197 g          708 t
ORIGIN      
        1 gcgccctggc tctcctacaa ccacgcacga aacatccccg ggttcgcagt cagccatgcc
       61 catctcgccg cctaccacca cgcccccgaa atccccgccg tgcgcaaggt cctcgactgg
      121 cacggccgca gtcgcaaaag caccagcttc ggccgcggtc tcgacctggc tcgtccgggg
      181 ctggatgcaa gctgggaaga agtgcgccgc tttcacgagg tcatacgcgc gcgaatcgcc
      241 caacagaaaa gctggcaagc cgaaacctac cgccgcaagc cgcgcaccat gctgctgctc
      301 atcggccgca tcttcggctt ccaggcgggc tggattccgc gcccggaggg agtcttcaac
      361 gaggacctga gcgaggaata cacgcgaaag attcgactgc gcgagggcgg cgccgaaatc
      421 gctccgtggc gcatagtcgt caacgactgg ctcgccgatt ggcacccgcg agccatgggc
      481 tgcgcttacg ccctcgcagt gctcggcgcc gcgctcagca tcgggctccg caaccgcgcc
      541 ggcctgctgc tggccggcgt cggcctgggc aatgcggccc tgctcgcagt cctgctccta
      601 cattcgcgcc gctatacctt gcctaccgaa ccattcgtaa tcctgctggg cgcctgggcg
      661 ctgtggcgca tattcgccgc ggccaagcgt cgactcggta tgggaaggga cgcacctcgg
      721 cgtggtgacc cttgacgcga cccgcctcaa agaaataatg accaagcgcc gcggcgactg
      781 acccaaaggt attctgtgag gagagcggag ctacgactag ccattctgca ccacccggcg
      841 tgatggacgc gcgttatgcc gccggccggc gccataagcc gcacttgtcc ttccgcctgc
      901 gctgtcgcgc ccgtgccgcc gccggcgcct atcggcgcta ccgcacgcac acacagccgc
      961 cgcgcgtgct cgatttcggc gccgccgagg gggctaccat ggtcgaagtg caccgcctgc
     1021 tcggcgccgc ggagtcaatc ggcgtcgagt acgccgcgga tctgatcgcc gccgcggagc
     1081 cgctgccggc cgactgccgc ctgctccagg gcgatgtcac cgctccgctg ccggaaatca
     1141 cccttggcca cttcgacctg gtaaccgccc tggccgtcct cgagcatctg gccgagccgg
     1201 ccgagctcat cgcccaggcg caccgcgccc tgcgccccgg cggcgtcttc gtcgcgacct
     1261 gcccggccgg tcactgggac aggctttccg ggcgcctcag gctgcaccag gacgaacacc
     1321 atgaaaccga gttcgaccgg caccgtttcg cggctgtggc ggaggccggc gggctcgtgc
     1381 cgcttagata tgagcgcttc atgttcgcac cgcttgggtt ccttccctac ctgcgtatcc
     1441 cggtgccggt cggcctggcg gaggcctgcg acggttgtgt acgctttctg cgcgtgtttg
     1501 aattcacgtt tgtcaaccag cttttcgtgg cgcgccgccc atgactgcaa gcgcacaagt
     1561 acagtctttg tcgcgcactg aaggtgcgaa cttgagaaat cgctacgccg aattggcgct
     1621 gccctacatt ccgcgactgt tgcagctcgt cgaccgcaac ccgtacagcc ccacctatgg
     1681 ctgcttcgat cgctcctact ggcactaccg cactatggac ttcccctgcg gcatgagcca
     1741 ggaattcgtg ctgccgctgg ccttggccta cgcaaacccg taccccggca acgtcttcca
     1801 caatgtcgca cgcgtgcgtg aactggccga agcgggcatt cgctacatga tccccagctc
     1861 gcacgccaac ggtacctgcg acgactactt ccccttcgag cgtgcgatgg gggccttggt
     1921 tttcagcctc tacgccgcca gcgaagccta ccaaatcctg ggcatgcgcg atgagcaggt
     1981 cgtggacctg ttcctcaaac gcatcaaaca cctggccgcc gagaacgaaa ccggccagct
     2041 cagcaaccat caggccttgg ctgccctcag cgcctacaac gtctatctca tcaccggcga
     2101 cgaccgctgc cgccgcctgg ccgaggaccg cgtcgcgctc accttgagct ggcaaaatgc
     2161 cgaagaaggc tggtttcagg agtacgaggg ggccgacccc ggctaccaca cctgcacgat
     2221 cgacttcctg gccaagctac agcagaagcg cggcgatacg gcgctgctaa agccgctttt
     2281 gaaagccgtc gatttcgcct ggcatttcat gcatcccgac gggtcatacg gcggcgaata
     2341 cggcagccgc aacacgtatc acttctaccc gcacgggttt gaattgctgg cgccgcactc
     2401 ggaaaaggcc gggcaaatcg tggacgcctt cctgcgcgga acggcgcgcg acaagcgcta
     2461 ccacaacgac gacgaccgca tgaccgccca ttacgtctac aacttcctcc aggcctggca
     2521 ggactaccac gagcaccgcc cgccgcccat cgctgccggc cgcagcacgc cccagaccat
     2581 ctggctgccc gcggccaaaa tggccgtttc ctggaacggc cgcgaggaaa acagcggcgg
     2641 ccgctacgtc gtcgccaacc tcagcaaggg cggcgtcctc aaggtgttcg acgccgccgg
     2701 gccgattgct tccgataccg gcctgatcgg cgaactcacc gacggccgcg tggtcgtctc
     2761 gcacctggtg cagcacgact caaccatcac ggccgacccg gcgcgcaacg aattcagcgt
     2821 caccggctgg ttctgccgcc gccgccgcaa tctgcccacc ccgttcaaat tcgtcatctt
     2881 ccggctattg ctgctgacga tcggccgctt taacgccaac ctgacgcgcc acctgctgca
     2941 gaagatactc atcaccggca aacccaaaac gcagttccgc ttcaagcgca cgctgcggct
     3001 ggagagcgac cgcgtcatcg tcaccgattt cgtcccgcgc gacatgccgc tcaaacggct
     3061 gagcgccggc gccgacgcca ccagcatcta cgtcgccaac agcaacgtct atcaggaatc
     3121 cgtgctctgc cgctggcggc acgtcgatcc ggccaccttg ccggtcgacg gcccctggcg
     3181 cgtctggcgg cgcgcttact accgcggcag cggagacgca ctcaaggatt gatcgcatgc
     3241 acaccggacc aggttgcctg gaagcggctg aaaaacagga tatggcacgc gtcaccgacc
     3301 tccgcgcccc gtcactccct ggagacgcgc cgatgatcat gatcgcccct aaacacgagc
     3361 tgacccccgg gctgtcagtc gttgtgcctt gctacaacga ggaggacgcc gtctgccaga
     3421 ccgtcgagca ggtcgcgcag gtgctggccg gcatgaactg ccccaccgaa atgatcttcg
     3481 tcgacgacgg cagcaccgac cgcactcccg cgctgttgga tgaactcgcc ggacgccatc
     3541 cgcgcctgcg ggtcgtccat aatcacgcca actgcggcta cggggcctcg ctcaaacgcg
     3601 gcatcagcgc cgcgcgctac gaccgcatca tcatcaccga cgccgacggt acctacccga
     3661 acgagcgcat cccggacctg taccacgcgc ttgatcatgc cgacatggtc gtcggagccc
     3721 gcatcgggcc gcgcgtgcac atcccgcttg cacgccggcc cgtgaagtgg ctgctgctga
     3781 aatactcccg ctggatggcc aaggccgaca tccaggacat caacagcggt ctgcgcggca
     3841 tctggacacg ccacgtccgc gccttttggt cgcttctgcc taacgctttc agcttcacca
     3901 ctaccatcac gctcgccacg cacatcaacc agctccgagt cgaatacctg cccatcgact
     3961 actatcagcg cgtcggcaag agctctatca ggccgctgca ggataccttc cgcttcttca
     4021 ccctggtctt ccgcaccatc atgtacttca gaccgctcca gatcttcggc acggccgccc
     4081 tgatcctgat gttcgctgcg gggctggcgg cggtgggggt gaaactctgg accggcctgg
     4141 tacccgacgt cacggtgatg tccctgt
//
LOCUS       DAOSYS010000293         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17417_length_4153_cov_4.859200, whole genome shotgun sequence.
ACCESSION   DAOSYS010000293 DAOSYS010000000
VERSION     DAOSYS010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17417_length_4153_cov_4.859200"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1456..2076)
                     /locus_tag="PLU99_14060"
                     /pseudo
     CDS             complement(1456..2076)
                     /locus_tag="PLU99_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2137..3522)
                     /locus_tag="PLU99_14065"
     CDS             complement(2137..3522)
                     /locus_tag="PLU99_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOO18215.1"
                     /translation="MKKWLILLGVVAVIGGVYYIARTKIRYTPVWLQPKFGVVTRGDI
                     SVPITAAGLIEPNLRIEVKAKASGEVIEVPVTEGTFVHKGDKLLVLKKDDEQRRYDAA
                     KADVDRAKAMLEQAKVGVSKAEANIITAEAEVERLTAEVEVSAEDLRHVEANHARGDE
                     TSQGLITMRGRHTVNLALKKAAEARLAIAYAALKEAEEAVKLQEAALRVAESSFGDAK
                     QRLDETTLIAQYDAIVTEVRVKMSEVIQGGMNTLTGGTPVMYLAEVSRKKVVARLDES
                     DYGRVLKISPIDALPEMPGLKEAVAATAAELERRSGTVKLTVDAFPEESFTGVIERVE
                     PQGKLNAGSAVIQYDVHVAITDPNAHKLPLGAQAQVEFTVESAKDALLVPAEAVKSHP
                     NQMDQRGIYVECPPEPGSNEQWGKKFIHCRLGITDGQNTQVVEALGGEKIEVGQKVYT
                     KLPASTDKAEP"
     gene            complement(3706..>4153)
                     /locus_tag="PLU99_14070"
     CDS             complement(3706..>4153)
                     /locus_tag="PLU99_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOO18216.1"
                     /translation="AAIRNHLERADLVIGAVLIPGARCPTLVPREYLKLMKPGAVIVD
                     VGVDQGGCCETTKPTTHEDPTYVVDGVLHYGVANMPGAVARTSTAGLTNATISYLVKL
                     AQRGFDRAIAADPGLAAGVNMAYGKITNQAVAEAFGLPYEPYDPKV"
BASE COUNT          660 a         1379 c         1350 g          764 t
ORIGIN      
        1 aagggcgtga agacgatgcc ggtttcgaga aagattattg caaacagcag cccgtatatc
       61 cacgacccat agttgcctac gaggtcaccg aggtgctggt cgaggtggat cagcgcgtca
      121 taagcgccct gcaaaaactc cactcaatac tcctgggcgg cgcccctggc gggccatgcc
      181 gcagtcggaa gtgcgtgggt tttaagcggg cgttacggcg acccagcggc ttacgctctg
      241 cagatcgcgg aggacgtccg ccgaaccgtg gagcgtatag gagctgcccg gcgtgagctg
      301 caccgggacc cgcgatttca tgcgatgttc ccacagccgg cctccacggc gcgacttgcg
      361 gagcgagggc cggtgctgct tgcgccagcg gctttggcgt accgcgcggg ggtccttgct
      421 gcaaacgcgg gtaagcgttt tgcgggccgc ctccgagcga ggcgtcgccg ttatctcgac
      481 acgaatcttg gtaccgggcg cgatgtccat ccagcaactc cgtcaagcct tagggaaatg
      541 gaatagttta ccacgcttgg ccgaggcgct caaggcccag gcgctcttgg gtgccacggc
      601 gttgggtgcc atgcccaagc gcccgcgaca gcggggcgcg ggcatgcggt caccagttgc
      661 cccgcacccc ccttccggtc acccccttcc tccgcggcct aattagcgca gtagtttgat
      721 gacgagtacg gcggcgatcg cgaccaacgg tccgaaggat gccaggcagc cggccgctgc
      781 gccgatccag atgcgccgcc cgccgtacca gttgcccgga atgtcgcgca tgcgcttgcg
      841 cgccacgcgg gcgacgacca cggcgacgat accggttacg aacccgccca gccagccgag
      901 gatcatgagc acggtgccga tcaccatcca gcgcggaatg gccttgaccc gctcttgtgg
      961 cggcagagat tccagattca agccgctggc tgcgacctgc acgaccaggc agaagatgat
     1021 tgccgccaga accacaacca gcgccagcaa cccgaggatg aaggccgcat tcgccccgcg
     1081 cgccagccgg cgcgggccga gatcgagagc tgaatcggcg ggttcggtgg gcggccgcgc
     1141 gacggtggcg gcgccgcgcg actcgatcgc ctcaggcagc actggcgcag tgcgtccggc
     1201 gccgggcggc tccgggcgct cggccgcgcg aatggccgca tggccgacaa ctgcggcgcc
     1261 cgcggtggcc gtaatcgtgc cggcgcccgc tcccgccgcc agtgtggggg cgcccgctcc
     1321 cgccgccagc gtgtgggcgc tcgcggtcac tgcgccgcgt gcggcgatcg ccggcgcgct
     1381 catctcgagt tcaaccggca tgcgaatctg caaaccatcc aatccggcct gcgattgcat
     1441 ctcggacgtg agccgatcgg cgacgatctt gccgtcgcgc atctgcacaa tgcgccgcgc
     1501 ttgaatggcc acttcgtgct cgtgcgttac cagcacgatg gttacgcctt gccggttcaa
     1561 ctcgcggaag atttccatga tctgctggcc ggtgcgcgaa tcgaggttgc ccgtcggttc
     1621 gtccgccagc agcaagggcg ggtcgttcac gatggcgcgc gcgattgcca cacgctgccg
     1681 ctcgccgccg gagagttcgt tggaacggtg atgggcccga tgcgccaacc cgacgcgctc
     1741 cagggcccgc agggccggcc cgcgcgtatt ggcgcgccgc gcgtagaaca gcggaacggc
     1801 cacgttgtcg agcgcggtga tgcggttgat caggttgaag gtttgaaaga cgaagccgat
     1861 ctggcggttg cgcatgttgg cgagctgccg gtcggacaag tcgctgaagt cgcgcccgtc
     1921 gaagaggaag cgcccggaag tcgggcggtc gagcccgccg atcaggtgca tgatcgtgct
     1981 cttgccgctg ccggaggcgc cggtgatggc gacgaactcg ccgcgctcga tggtcaggtc
     2041 tacgccgtcc agggcgcgga ccagcgtgtc gcccatgcgg tagagcttgg tcagtccttt
     2101 aacttcgatc agcgccaact tcgcgactcc gcccgcttac ggctcggcct tatccgtact
     2161 cgccggcagt tttgtgtaga ctttctggcc cacctcgatc ttctcgccgc ctagagcctc
     2221 aacgacctgc gtgttctggc cgtcggtgat gccgaggcgg caatggatga atttcttgcc
     2281 ccactgttcg ttcgagcccg gctccggggg acactcaacg taaatgccgc gctggtccat
     2341 ctggttcggg tggctcttga ccgcttcggc cgggaccagc agcgcgtctt tggcgctctc
     2401 gacggtgaac tcgacctgcg cctgggcccc cagcggcagc ttgtgggcgt tcgggtccgt
     2461 gatggccacg tgcacgtcgt attgaatcac ggccgagccc gcgttcagct tgccctgcgg
     2521 ctcgacgcgc tcgatgaccc cggtgaagct ctcttccggg aaggcgtcga ccgtgagttt
     2581 gacagttccg gagcggcgtt ccagctctgc ggcggtagcg gccaccgctt ccttgaggcc
     2641 gggcatttcg ggcagggcgt cgatgggcga gatcttcagc acacgaccgt agtcggactc
     2701 gtccaggcgc gccaccacct tcttgcgcga cacttccgcg aggtacatga ccggggtgcc
     2761 gccggtgagc gtgttcatgc cgccctgaat gacctcgctc attttcacgc ggacttcggt
     2821 gacgattgcg tcgtactggg caatcagcgt ggtttcatcg agccgttgtt ttgcatcgcc
     2881 gaacgagctt tccgccacgc gcagggcggc ctcctggagc ttgaccgcct cttccgcttc
     2941 cttcagcgcg gcgtacgcga tcgcgaggcg cgcttcggcc gcctttttca gcgccagatt
     3001 caccgtgtgc cggccgcgca tggtgatcag accctggctg gtctcgtccc ctcttgcgtg
     3061 gttggcctca acgtgcctga gatcctcggc gctgacttcc acctcggccg tcaggcgctc
     3121 gacctcggct tcggcggtga tgatattggc ctcggcttta ctgacgccca ccttggcctg
     3181 ctcgagcatc gccttggcgc gatcaacgtc tgccttggcg gcatcgtaac gccgctgctc
     3241 gtcgtccttt ttgagcacca ggagcttgtc gcccttgtga acgaaggtcc cttccgtgac
     3301 cggaacttcg atcacctcgc cggaggcttt ggccttgacc tcgatgcgca gattgggctc
     3361 gatcagaccc gcggccgtaa tgggcacgct gatatcaccg cgcgtgacaa ccccgaattt
     3421 gggctgaagc cagaccggcg tgtagcgaat cttcgtgcgg gcgatgtaat agacgccgcc
     3481 gatcacggcg accacaccca agaggatcag ccatttcttc atgcttgtgg tcctgctttg
     3541 aatgaaccgc aggcgcaaaa cctcgtagga ccgcaggcgc aaaaccttgc aggaccggtt
     3601 cgaagccggt cccacactgc acacgcttgt ggcacactta gcgacaccgg cacccggccg
     3661 gcggaattac cgcgggacgc ctgtgccacc tcggcgcgag ccccactaca ccttggggtc
     3721 gtatggctcg tacggcaggc cgaacgcctc ggcgacggcc tggttggtga tcttaccgta
     3781 agccatgttg actcccgccg ccagtccggg gtcggccgcg atggctctat caaacccgcg
     3841 ttgggcgagc ttgacaaggt aggaaattgt cgcgttcgtg aggccggccg tgctcgtgcg
     3901 cgcgaccgcg ccgggcatgt tggctacgcc gtagtgcagc acgccgtcca cgacgtaagt
     3961 gggatcctca tgagtggtgg gcttggtggt ttcgcaacag ccgccctggt ccacgccaac
     4021 gtcgacaatc accgcgccgg gcttcatgag cttgaggtac tcgcgcggca ccagcgtcgg
     4081 gcagcgcgcc ccgggaatca gcacggcccc gatgaccaag tcggcccgct caagatgatt
     4141 gcgaatcgcc gcc
//
LOCUS       DAOSYS010000294         4140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17493_length_4140_cov_5.266830, whole genome shotgun sequence.
ACCESSION   DAOSYS010000294 DAOSYS010000000
VERSION     DAOSYS010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17493_length_4140_cov_5.266830"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLU99_14075"
     CDS             complement(<1..494)
                     /locus_tag="PLU99_14075"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18217.1"
                     /translation="MPAKKTRPNAANQHAARPRPGAIADNQPGAQGRAERAANPRGLP
                     RHPGHAPGASAEQPHPLPPLRREVGEVFGLYGLSVLLFSLIFPPGGIWPLAFVCLAPW
                     AIATCRTRRAWLVHWSSLLFGIAFFLINLRWLMPVTGLGYVALCFYLAIYWTLAAWAL
                     RTAQR"
     gene            complement(1021..2508)
                     /locus_tag="PLU99_14080"
     CDS             complement(1021..2508)
                     /locus_tag="PLU99_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOO18218.1"
                     /translation="MNDEQELARLLNRGHTCICVSTLEEEYTLGLIRNAALNADRGLW
                     VWSIAGGVQDGLFTSAPCVPETESPVAGLTYFADLPERSLCVALDLASHLRDERTLRV
                     LRNLLQQFQSTRRQLILLDWKEELPQVIRDYATEFRLSLPDDAEVEQIVAATLRKARE
                     DGPLEVSIRRSTLRVMIRNLRGLPRRQIERIINDAVVLDRRLDDSDLNAVLAQKRQIL
                     QHGSLLQFVQTPVDLSEIGGLRRLKAWLEQRAPATHEHAKDYGLVAPRGVLMLGVQGA
                     GKSLCAKAIATAWQQPLLRMDVGALYDRYIGESERRLRDTFAQAERMAPIVLWIDEIE
                     KAFASAASHNTDGGLSQRMFGSLLTWMQEHTAPVFIVATANNIDALPPELLRKGRFDE
                     VFFVDLPNEEVRRQIFGIHLRKRRRDPKQFDLQALAVASDGFSGAEIEQAVIAGLHRA
                     FSASTELSTDHILQAIMGSPPLSVTMAEHVAALRQWAIGRCVPAD"
     gene            complement(2726..3340)
                     /locus_tag="PLU99_14085"
     CDS             complement(2726..3340)
                     /locus_tag="PLU99_14085"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOO18219.1"
                     /translation="MAYELPPLPYAFDALEPPIDARTVEIHYTKHHKTYLDNLNKALE
                     AHPDLSRKPIDELLRDLSQIPENIRQTVINNGGGYANHNLYWEVMGPKKGGEPRGQLA
                     NAINDAFGGFAKFKEQFSQAALTRFGSGYAWLTFDPNGKLTITQTLNQDSVVSIGHKP
                     ILIIDVWEHAYYLNYQNRRADYIAAWWNVVNWDAAEQRYRAAKR"
     gene            3581..4099
                     /locus_tag="PLU99_14090"
     CDS             3581..4099
                     /locus_tag="PLU99_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18220.1"
                     /translation="MQSWQILREAVEQVGAKTLAAKLRVSAALIYKWSQETGPRGTGS
                     GALNPLDRVRSILEITGDTRIVRWLCQAAGGFFVESPQLDRLNQGAGLLASTQRVIEE
                     FSKVLRTISRSIENDGQITPDESDRIRQSWEELKSCGERFVVACERGAYAERSSSADG
                     TQPAQSWSVGGG"
BASE COUNT          777 a         1262 c         1347 g          754 t
ORIGIN      
        1 cgctgcgccg tgcgcaaggc ccaggccgcc agcgtccagt agattgccag gtaaaagcac
       61 agcgcgacgt agcccagccc cgtcaccggc atgagccaac gcaggtttat taggaaaaag
      121 gcgatgccga agagcaggct tgaccagtgc accagccagg cccggcgcgt gcggcaggtc
      181 gcgatcgccc agggcgccag gcacacaaac gccagcggcc agattccgcc gggggggaat
      241 atcagtgaaa acagcagcac tgacaggccg tacaggccga aaacctcgcc gacctcgcgc
      301 cgcagcggcg ggagcggatg cggttgctcc gccgacgcgc ctggtgcgtg cccaggatgg
      361 cgtggaagtc ctcgcgggtt ggccgcgcgt tccgcccggc cctgcgcgcc cggctgattg
      421 tcggcgatcg cgcccggccg tggtcgcgca gcgtgctgat tggcagcgtt gggtcgtgtc
      481 ttcttggctg gcacggacac tccgatcggc ccggctcaaa ggcaccgccc cggttgatca
      541 acggaagcgg caagccggcg attgtgcggt gcgctgggag cagcgtcaag cgcgtatcaa
      601 ggatcaaggg cggcaccttc ccacgttccc acattcccac tttcccacag gcatgcgtgg
      661 gaaagtggga atgtgggaaa gtgggaacgt gggaatgggg attcgccgaa aagcctccgc
      721 agccgcgcaa cgcccgccac gaccgcagcg caccgccgcc tctggcgtac ccgatccgag
      781 cccgcctgat ccgtgcccgc agcgccagcg agggcaactc ggtcccggcg gatacgagcc
      841 cgcggcgcca gcgagggcac cggtatcccg atccgagccc gcagcgccag caagggcaat
      901 ttgttctgat ccgagcccaa cccccgctca gcaaacgcac ctggtctggt caggcgtgcg
      961 acctgcgcaa ttggcggcac tctggtcgca ttccggctcg acacgagccg agtctgctac
     1021 tcaatcggcc ggaacgcagc ggccgatggc ccactgtcgc aatgcggcta cgtgttcggc
     1081 cattgtcaca gagaggggcg gcgagcccat gattgcctgc aagatgtgat cggtggacag
     1141 ctcggtgctc gcggagaagg ccctgtgaag acccgctatg acggcttgct cgatctcggc
     1201 cccgctgaag ccatcggacg cgacggctag cgcctgcaag tcgaactgct tggggtcgcg
     1261 gcggcgcttg cgcaggtgaa ttccgaaaat ttgccgccgc acttcctcgt ttggcagatc
     1321 gacgaagaag acctcgtcga agcgcccctt gcgcagcaac tcgggcggca acgcgtcgat
     1381 gttattcgca gtggccacga taaaaaccgg ggccgtgtgc tcctgcatcc atgtcagcag
     1441 tgagccaaac atgcgctgcg acagcccgcc gtcggtgtta tggctggcgg ccgacgcgaa
     1501 cgccttttcg atttcgtcga tccagagcac gattggcgcc atgcgttcgg cctgggcgaa
     1561 cgtgtcgcgc agtcgccgct ccgattcgcc gatgtagcgg tcgtacaggg cccccacgtc
     1621 catccgcagc agtggttgtt gccaggcggt cgcgatggct ttggcgcaca ggctcttgcc
     1681 cgctccttgc accccgagca tcaacacgcc gcgcggggcc accagcccgt agtctttcgc
     1741 atgttcatgc gttgccggcg cgcgctgctc cagccatgcc ttcagccgcc gcaggccgcc
     1801 gatctcgctc aggtcgacgg gcgtctgtac gaattggagc aagctgccgt gctggagtat
     1861 ctgtcgcttc tgcgccagga cggcgttgag atcgctgtcg tccaggcggc gatccagcac
     1921 gaccgcgtca ttgatgatgc gttcgatttg acggcgcggc agcccgcgca ggttgcggat
     1981 catgacgcgc agcgtgctgc gcctgatgga tacttccagc ggtccgtctt cgcgggcttt
     2041 gcgcagcgtg gccgcgacga tttgctccac ttcggcgtcg tccggcagag aaagccggaa
     2101 ttcggtcgcg tagtcccgaa tgacctgcgg cagttcctct ttccaatcga gcaggatgag
     2161 ttgccgccgg gtggactgga actgttgcag cagattgcgg aggacacgga gcgtacgttc
     2221 gtcgcgcagg tgcgaagcga ggtcgagcgc gacgcaaagc gaacgttcgg gtaggtcggc
     2281 aaaatacgta agacccgcga cggggctctc cgtttccggc acacacgggg cagatgtaaa
     2341 cagcccgtcc tgaacgccac cggctataga ccagacccac agcccccgat cagcattcag
     2401 ggccgcattg cgaatcagcc ccagcgtgta ttcctcttcg agcgtggaca cacaaatgca
     2461 ggtgtggcca cgattcagca ggcgcgccaa ctcctgctcg tcattcatgg ttgttcatcc
     2521 gctgtttact cccaacgaga gtataacgtg aggagggtat cgagggggtt caggacaggc
     2581 cacgcgagga tcgagggatc gagaggggct aagcgtgggg ggcgcgtcct ggcaagaacg
     2641 cgccccggcg tccaaaatcg gcgcgcgata gaacgcgaag agcgtgctgt cgcggcttgg
     2701 cacgattcaa gtcatcgtgg cgaattcagc gcttcgcggc gcgataccgt tgctcggcgg
     2761 catcccagtt gaccacgttc caccaggcgg cgatgtagtc ggcacggcgg ttctgataat
     2821 tcaagtagta ggcgtgttcc caaacatcaa tgatgagaat cggtttgtgg ccaatcgaga
     2881 ccaccgagtc ctggttgagc gtttgggtga tggtcagctt tccattcggg tcgaatgtca
     2941 gccaggcgta accgcttccg aagcgcgtca acgctgcctg gctgaactgc tccttgaatt
     3001 tcgcaaaccc gccgaaggcg tcgttgatcg cgttggccaa ctggccgcgc ggctcgccgc
     3061 ccttcttggg ccccataact tcccaataca ggttgtggtt cgcatatccg ccgccgttgt
     3121 ttatgacggt ttggcggatg ttctcgggga tttgggacaa atcgcggagc agttcgtcga
     3181 tcggcttgcg cgacaagtcc ggatgagcct caagggcttt attgaggtta tcaaggtacg
     3241 ttttgtggtg cttggtgtag tgaatctcta cggttcgcgc gtcgatggga ggctcgagcg
     3301 cgtcgaacgc gtacggaagg ggcgggagct catatgccat agttcacctc cattatggga
     3361 ccagaaaatc ccctgcgggg agcacgaaag cggttccatg cagggcttgg gaccgaacac
     3421 ctggaccaac gccaccgtag attattgcgc ccttgacaca tggattcaag ccttcccgcc
     3481 gccgcacagg attgacggag accgccaact ttcggataat accggtccaa ctctgaagtt
     3541 gcgtctctgg ccggcatcgt ccggcgtcgg aggactgaac atgcagtctt ggcaaatatt
     3601 gagagaggcg gtcgagcaag tcggggcgaa aactttggct gcgaagctgc gcgtcagcgc
     3661 cgcgctcatt tacaaatggt cgcaggagac agggccgcgc ggtacgggct ccggggcctt
     3721 gaacccgttg gatcgcgttc gcagcatcct cgagattacg ggcgacacgc ggatcgtgcg
     3781 ctggctttgc caggcggccg gcggcttttt cgtggagagc ccgcagctcg accggctcaa
     3841 tcagggggcc gggctgctgg cttccacgca acgcgtgatc gaggagttca gcaaggtcct
     3901 acgcacgatc agccgcagta ttgaaaacga tgggcaaatc acgcccgacg aatccgaccg
     3961 gatacgccaa tcctgggagg aattgaaatc ctgcggcgaa cgcttcgtgg tggcttgcga
     4021 gaggggcgct tacgccgaac ggagttcgag cgctgacggc acgcagcctg cgcagagctg
     4081 gagcgtgggt ggcggataag cgtcacaaaa tcagcgtctc gtttgacaac ttgacgcgta
//
LOCUS       DAOSYS010000295         4115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17626_length_4115_cov_4.611576, whole genome shotgun sequence.
ACCESSION   DAOSYS010000295 DAOSYS010000000
VERSION     DAOSYS010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17626_length_4115_cov_4.611576"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1057)
                     /locus_tag="PLU99_14095"
     CDS             complement(215..1057)
                     /locus_tag="PLU99_14095"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOO18221.1"
                     /translation="MLAALPLEQVEPTPYQRKVSDAHVKRLSDVIARTGRFLDPVIAV
                     RVGPKQYQTPNGGHRTGALKLQGARSITALVVTEPEVARLILALNVEKAHNLREKSLE
                     VIRLARELAAITAGAAPAARGAAPAAATGIAPATAAREDEFTLEFEEPAFLTLGLCYD
                     ANGRFGGGAYHPILKRVDEFLHQPLPKALETRQQYSDALLELDGLVAELVAGLKQRGL
                     ESPYLKSFVVARLNPLRFRRGATLPIEETLKKMTEAARKFKPDKITTADLARTGAAPP
                     EEPS"
     gene            complement(1354..3375)
                     /locus_tag="PLU99_14100"
     CDS             complement(1354..3375)
                     /locus_tag="PLU99_14100"
                     /inference="COORDINATES: protein motif:HMM:NF017043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18222.1"
                     /translation="MIVTPDTIPPESRRTWLLDERRQLAAHQALCAARETRWSWARLV
                     VFALALVPWLVIPQHAAFAGGLTLTALVLFALTIRGHHTLLAQREHADRLLRVVDEAL
                     QRVGGAVVCVRDWQRPPDAPEDDALLPNLLQRGPTWALTPQERDDLDLFAAPVGIYGL
                     LNRTSTAPGARRLRDMLENPLLDPQRIRRRQSAVQWLAHNAADRLHIMAGCAALRGED
                     RRLARLMHALADAQPLKLPLPAWLLVSWAMLSAAFAIFVIARIVAADFTWVRWLLLLL
                     AVNGALYARLRGRLNAAFAPWQDAAWAARALLTAARCAARHLPAVTNRSGVADCCGAE
                     HQGAAEPSELAVLAEHCNAIIQPGALPTLYRRSGWVEGGGLPRLLANILVFADVFLAR
                     RLLACALPHRGALLAGISAIAELEALCSFAAFAWEQPVACYPAMVAGTKLQMVEGVHP
                     LVPPERVVPNDASLDENVRLWIITGSNMAGKSTFLRTVALNTLLAQAGGIATARELTL
                     SPVRLITDLRTRDSLAERESYFLAEVRHVRRMVLPPAGDAPVLGIMDEPFRGTDSQDQ
                     TAASLAVVQQLLASPHLFLVATHNRQLTTLAEGGPTATTAPNGRAARNFHFRENLAGD
                     ELVFDYRLYPGPAQTRNALRILAREGYPPELVERAQAWLQNATGRQGAI"
     gene            3764..4006
                     /locus_tag="PLU99_14105"
     CDS             3764..4006
                     /locus_tag="PLU99_14105"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOO18223.1"
                     /translation="MGSARLVFKQQDGRPEVRAGRVLNISPGGVQIWQAEDINWGTEV
                     VVQVRVAERDMLLFGRVAHCTEAVGGFKVGVELQFS"
BASE COUNT          692 a         1189 c         1517 g          717 t
ORIGIN      
        1 atgcccgcgc ccgcaacgcg ggcttgggca tggcacccgc acgagcggcg gatgagagat
       61 taccggcggt attccggggc gacacgcccc ggctctgctc gcacaagggc gaattgcggc
      121 gtgttggcat cattccgggc cgacacggcc cggctctgct cccagtgaat tgggacgcgt
      181 tggcatcatc ccggggcggc acgccccggc tctgctatga gggttcttcg ggcggcgcgg
      241 cgccggtgcg ggccaggtcg gcagtggtga ttttgtctgg tttgaacttg cgggcggctt
      301 cggtcatttt ctttagcgtt tcttcgatcg gcagcgttgc gccgcgacgg aagcgcagtg
      361 ggttcaagcg ggccactacg aagctcttga gatacgggct ttccaggccg cgctgcttca
      421 acccggccac cagttcggcc accaaaccat cgagttcgag caaggcgtcg gagtattgct
      481 ggcgcgtttc aagcgccttc ggcaagggct gatgcaggaa ttcgtcgacg cgcttgagga
      541 tcgggtgata ggcgccgccg ccgaagcggc cgttggcgtc gtagcataga ccaagggtga
      601 ggaaggcggg ctcttcgaat tcgagcgtga attcgtcttc gcgcgcggcg gtggcggggg
      661 cgatcccagt ggcggcggcg ggcgcggccc ctctggcggc gggcgcggcc ccggcggtga
      721 tggccgcgag ttcgcgcgcc aggcggatga cctcgagcga cttctcgcgc aggttgtggg
      781 ccttctcgac gttcagcgcc aggatgaggc gggccacttc cggctcggtg acgaccagcg
      841 cggtgatcga gcgggcgccc tgtagcttga gggcgccggt gcgatggccg ccgttcggcg
      901 tttggtattg cttcgggccg acgcgcacgg caatcacggg gtcgaggaag cggccggtgc
      961 gagcgatcac gtcagacagg cgtttgacgt gcgcgtcgga gactttgcgc tggtagggcg
     1021 tcggctcgac ttgttcaagc ggcagcgcgg ccagcacaag ccagtgcccg ccgagcgggt
     1081 cgcgataggg cgcgatgacg gcgccaccgt cggcttgaat ttcgcggcag agttcctcaa
     1141 ccgccttggg cggtgacgtg cagacctcgc gcggggtgag cccgacgggt ccgctgggtg
     1201 ttttccggcg gcgcgtggtt ttcttgacag ctttggccat ggcacggctc ccgagagcgc
     1261 gaacggacag atatggtaag cgggaaaacg gtcacgaggt aagcacgacg ggccgcgttt
     1321 gacgacggga atactcgcca aagtgggctt gcgtcagatt gcgccttggc ggccagtcgc
     1381 gttctgcagc cacgcctgcg cgcgctcgac cagctccggc gggtagcctt cgcgcgccag
     1441 gattcgtagt gcgttgcgcg tctgcgcggg gccgggatac aagcggtagt cgaagaccaa
     1501 ttcgtcgccg gcgaggttct cgcggaagtg gaagttgcgc gcggcgcggc cgttcggtgc
     1561 cgttgttgct gttgggccgc cttcggctaa cgtcgtcaac tgccggttgt gcgtggccac
     1621 caggaacaaa tgcggtgagg cgagcaactg ctgcactacc gccaggcttg cggcggtttg
     1681 gtcctgcgag tctgttccgc ggaatggttc gtccattatg cctaaaaccg gggcatcacc
     1741 cgcgggcggg agaaccatgc gccgcacgtg ccgcacttcg gccaggaaat agctttcacg
     1801 ctccgccaga ctgtcgcgag tgcgcaagtc tgtaatgagc cgcaccggcg aaagggtcag
     1861 ttcgcgggcg gtcgcgatgc cgccggcctg cgcgagcagc gtattgagcg cgacggtgcg
     1921 gaggaacgtg cttttgccgg ccatattgga gccggtgatg atccacaggc gtacgttttc
     1981 gtccagtgat gcgtcattgg gcactacgcg ctcgggtggc accagcggat gtacgccctc
     2041 gaccatctgc agcttggtgc ccgcgaccat cgccgggtag cacgcgaccg gctgttccca
     2101 cgcgaatgcg gcgaaggagc acagggcctc taactccgcg atggccgaga tgccggccaa
     2161 cagtgcgcca cggtgtggca gtgcgcaggc cagcagccgg cgggcgagga acacgtcggc
     2221 gaaaacgaga atgtttgcca gcagtcgcgg tagcccgccg ccttcgaccc agcccgaacg
     2281 acgatacagg gtgggtaagg ctccgggctg gatgattgcg ttgcagtgct ccgcaaggac
     2341 tgccagctca ctcggctcgg cggcgccctg gtgttcagcg ccgcagcagt cggcgacgcc
     2401 ggagcggtta gtaacagccg gtaaatgcct ggcggcgcag cgtgcggcag tcagtagggc
     2461 gcgggccgcc caggctgcgt cttgccaggg agcgaaggcg gcattcagcc gcccccgcag
     2521 acgggcgtat aacgccccgt taaccgccag caacagcagc agccagcgaa cccacgtaaa
     2581 gtcagcagcg acgattcgcg cgatcacgaa aatagcgaaa gccgcactga gcatcgccca
     2641 tgagactaaa agccaggccg gcagcggcaa cttgagcggc tgcgcgtctg ccagggcgtg
     2701 catcaatcgt gcaagtcgcc gatcctcgcc gcgcagcgcc gcacagccgg ccatgatgtg
     2761 cagccggtcc gcggcgttgt gtgccagcca ttgcacggcg ctttggcggc gccggatgcg
     2821 ctgtgggtcg aggagcgggt tttcaagcat gtcacgtagc cggcgtgcac cgggggcggt
     2881 ggaagtgcga tttaacaggc cgtaaatgcc gactggggcg gcgaagaggt cgaggtcgtc
     2941 gcgctcctga ggggtgagtg cccaagtcgg gccgcgctgc aggagatttg gcaacagggc
     3001 atcatcctca ggggcatcgg gcgggcgttg ccagtcgcgc acgcagacga cggcgccacc
     3061 gacgcgctgt aaggcctcgt cgaccacgcg gagcaggcgg tcggcatgct cacgctgcgc
     3121 gagcagggtg tgatggcccc ggatcgtcag ggcgaacagg accaaagcgg tgagcgtcag
     3181 tccgcccgcg aatgcggcgt gctgaggaat tacgagccat ggcaccaggg ccaacgcgaa
     3241 tacgaccagg cgggcccagg accagcgggt ttcgcgcgct gcgcaaagcg cctggtgagc
     3301 ggccagttgg cgacgctcgt ccagtagcca ggtacggcgg gattcgggag ggattgtgtc
     3361 cggggtgaca atcatgtggc ggattgtaag cgttcgccgc agtcgggggc atactggatg
     3421 gcgttcagca tattcgaggc attacggcct aggcatgccc gcgcttacag cttgggcatg
     3481 gcacccaaca tggcatccgg cggggcgtcg cggctggtgg gttgttggcg gcgtttttag
     3541 cgtgtgcacg cttacaggtg catagaattg tgagcggtta agttgggccg agtggttgtc
     3601 cgataaaaga catgggcggt aattcgggcg gggttaggga gtccgacctg tgttgggatt
     3661 ggccgaaaag acgagtcgca aagactggga agcgcttgtc accctgcatc cgaagcagag
     3721 ctttaccgag caccgacggc ataacgggtg gcgaagcccg gccatggggt cggcacgatt
     3781 agtgttcaaa cagcaggacg ggcggccgga ggtgcgcgcc gggcgtgtgc tcaatatttc
     3841 gcctggtgga gtccagattt ggcaggcgga agacattaat tggggcacgg aggtggtggt
     3901 ccaggtgcga gtggccgaac gggatatgtt gctgttcggc cgcgtggcgc attgcaccga
     3961 agcggtaggg gggttcaagg ttggcgtgga gttgcagttt tcctgagccg cgacgggtga
     4021 accggggcgg gcaactgcgc gaaggcattt taggcggcat tccggggcgg cacgcgccgg
     4081 ctctgctctg cgatgcgagg ttactgccga tggca
//
LOCUS       DAOSYS010000296         4098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_17712_length_4098_cov_4.008657, whole genome shotgun sequence.
ACCESSION   DAOSYS010000296 DAOSYS010000000
VERSION     DAOSYS010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17712_length_4098_cov_4.008657"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLU99_14110"
     CDS             complement(<1..539)
                     /locus_tag="PLU99_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18224.1"
                     /translation="MADERTGIKYIGKDATYKDRMFGTGLVWRQGEILPISIDLAKDF
                     LKHPALFAEEPGFSYLGVTRSAQGVGNIIPMVGGKYAPTQADLLRLFPLPTHRVIADP
                     WTVNIAGATKPAGATITIEADANEYGGSVIRIDLPAGVVGGAITLPINPDITGAYPKA
                     LPETAWRMNVSDWSAVSRL"
     gene            complement(605..>4098)
                     /locus_tag="PLU99_14115"
     CDS             complement(605..>4098)
                     /locus_tag="PLU99_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015975462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HOO18225.1"
                     /translation="LGRLTGAQSARLGFFFTSSEKIASGYASALGNDVSVAEESRRYE
                     NSKKANRELAAAEKELTKYGYQSYTWENSDERDSSESEAKQANRENALEAMRAYKAAE
                     TSLADANIQPGGVVYPVYVRILDPEITDQDGKLYRDWKFYDFVGDAKNAGKDGAIIKN
                     TYDSANMWNAEVTEASDVFIVFSPDQIKSATGNAGTFDPASPDIRFSRSATLRAATKA
                     ATSALNDTFTAPGKLSWWHRSIGTMYNLAERSPAFAPVFKAAQGFIDDVSHYANDAAE
                     MAPKLLPKLDAWRDIWKSPISAADNVAIAKPVFEGTLPWGRDASGRAVKVADMSEAAE
                     KMSIRQKADRLMGADAISGNVHKMWLGLPVDQYEKNINTSYEGAFLQPGIVWKDAELR
                     SLFNLSDKQIELYREFRRATDRSLDTMARADMLRYGGKDAKPLRDAVMDAPDVEAAAI
                     LLRDHFGELAKMDPERETLLLDTANAMMDRSDKVLKLQKQGYAPLSRFGRYTVDVVTD
                     GRREYFGLFETAREANQMAARMRREFGDSAVTQGTLSQEAFKMFAGITPETLELFGNA
                     LGLDSTGDEARDQAFQEYLRLTKSNRSAMKRMIHRQGIAGYSEDVARVLATFVYSNAR
                     QTAAGLNMGDLGEAVQAIPKEQGELKDVAFGLADYVKNPQEEAQAIRGLLFAQYLGGS
                     IASAFVNLTQPAAVTFPWLSQFGGAKAAAAQLAKAAADMAKKRYEPDLVRALHAAEED
                     GTVSPQEVHQLMAQAQGGGSLRTGDGTKRGDALAMASNGFKRMAFAWGKVFGMAEQVN
                     RRITFIAAYRTAQARGMADPAQFARKAVQETQFVYSKASKQQWGRGAVGGTLMTFKTY
                     SIAYLELLHRMATQGGPEGKKAALLALGMLMLMGGAGGLPFAEDAEDVAEALARMLGY
                     NISVKKARQELLEDTFGRGIAAFIDKGITGLPGVPLDVSGRLGMGNLIPGTGLLQPKT
                     DHTSDVLEIAGPAGDMAKRAFDAGGRLLKGDIAGAALQASPTAGRNLAKGIDMATTGM
                     YRDAKGYKVLDTDMLEAALKSVGFQPKSVATIQKANAEAQQAKNFYSMRAQSIRMKWA
                     QGVFEGDMDKVQEARDEIADWNAKNPDQRMVISVPSVMKKVQEMRKPKDERIAATAPK
                     AMRANLRAEFEKAREGL"
BASE COUNT          700 a         1369 c         1147 g          882 t
ORIGIN      
        1 tagagccgac taacggcgga ccagtcggag acattcatgc gccaggctgt ttcgggcaga
       61 gctttcgggt acgctccagt gatgtccgga ttgatcggca gcgtgatcgc accgccgaca
      121 acgcccgcgg gaaggtcgat ccgtatcacg ctgcccccat actcgtttgc gtcggcttca
      181 attgtgatcg ttgcgccggc gggtttcgta gcccccgcaa tattgacggt ccaggggtcg
      241 gcaatgacgc ggtgcgtcgg caaagggaac agcctcaaca gatcagcttg agtcggcgcg
      301 tacttgccgc cgaccatcgg gatgatattt ccaaccccct gggccgagcg ggttaccccc
      361 aaatagctaa accccggctc ctcagcaaac agagccggat gcttcagaaa gtccttcgcc
      421 agatcaatcg aaataggaag aatctcgccc tgtctccata ccaagcccgt cccgaacatg
      481 cggtccttgt aggtggcgtc cttcccgatg tatttaatcc cggtgcgttc gtcggccatg
      541 attgctttgc ctcaattgaa tgaattgagg tgatcatggt tccgacgcac gcgccgggct
      601 aaccctacaa cccttcccgc gctttctcga actcagcccg cagattggcc cgcatcgcct
      661 tcggtgcggt ggcggcaatg cgctcatcct tcggtttgcg catctcctgc accttcttca
      721 tcaccgatgg aacggagatc accatccgct gatcagggtt cttcgcgttc cagtcggcta
      781 tctcgtcacg cgcctcctgc accttgtcca tgtcgccctc gaaaacccct tgcgcccact
      841 tcatgcggat gctctgcgct cgcatgctgt agaagttctt cgcctgctgg gcttcagcat
      901 tggccttttg aattgtggcc acgctcttgg gctggaagcc caccgacttc aaggccgctt
      961 cgagcatgtc agtgtcgagc accttataac ccttggcgtc ccgatacatg ccggtcgtag
     1021 ccatgtcgat gccctttgcc aggttgcgcc cggccgtggg tgacgcctgc agcgcagcgc
     1081 cggcaatgtc gcccttcaac agccggccgc ccgcatcaaa cgcccgcttt gccatgtcgc
     1141 ccgctggccc cgcgatttcg agcacatcgg atgtgtggtc ggtcttgggc tgcagcagtc
     1201 cggtacccgg aatcaggttg cccatgccaa ggcggcccga gacatccagc ggcacgcccg
     1261 gaaggccggt aattcccttg tcgatgaacg ccgcaatacc gcgcccgaac gtgtcttcca
     1321 gaagttcttg gcgagccttc ttcacgctga tgttgtagcc cagcatccgg gccagcgcct
     1381 cggcaacgtc ttcggcatct tcggcgaacg gcaagccccc ggctccaccc atcagcatga
     1441 gcatacccag cgccagcagt gcggctttct tgccctccgg cccgccctga gttgccatcc
     1501 ggtgcagcag ctcaaggtag gcgatggagt aggtcttgaa ggtcatcaag gtgccgccca
     1561 ccgccccgcg accccattgc tgcttggatg ccttcgagta gacaaactgt gtttcctgca
     1621 cggctttgcg ggcaaactgc gccgggtcag ccatgccgcg ggcctgcgcc gtccggtagg
     1681 cggcaatgaa ggtaatccgg cggttcacct gctccgccat cccaaacacc ttgccccacg
     1741 caaaagccat ccgcttgaag ccgttggagg ccatcgccag cgcatcgccc cgtttcgtgc
     1801 cgtcgccagt cctgagcgat cccccgccct gcgcctgcgc catcagttga tgcacctcct
     1861 gcggcgacac cgtgccgtct tcttcggcgg catgcagcgc ccgcaccaag tccggctcgt
     1921 aacgcttctt cgccatgtct gccgccgcct tagcaagctg cgccgccgcc gccttggcgc
     1981 cgccaaactg cgacagccac gggaacgtta ccgccgccgg ctgcgtcagg ttcacgaaag
     2041 ccgacgcaat cgagccgccc aggtactgcg caaacagcag tccgcggatt gcctgcgcct
     2101 cttcctgcgg gttcttcacg tagtcagcca gcccgaaagc cacgtccttc agctcgccct
     2161 gctccttcgg gatcgcctgt accgcttcgc ccaggtcgcc catgttcaag ccggcagccg
     2221 tctgccgggc gttggaatag acgaaggtcg ccagcacccg cgccacatcc tccgaatagc
     2281 cggcgatgcc ctgacggtga atcatccgct tcatcgcgct gcggttgctc ttggtcaggc
     2341 gcagatactc ctgaaacgcc tgatcgcgcg cttcgtcgcc ggtcgagtcg agccccagcg
     2401 cgttaccgaa tagctccagc gtctcgggcg tgatgccggc gaacatcttg aacgcctctt
     2461 gcgatagcgt gccctgcgtc acggcgctgt cgccaaactc ccggcgcatc cgggccgcca
     2521 tctggttggc ctcccgcgcc gtttcgaaca ggccgaaata ctcgcggcgg ccgtccgtca
     2581 ccacatccac cgtgtagcgc ccaaagcgcg acagtggggc gtagccctgc ttttgcagct
     2641 tcagcacctt gtcggaacgg tccatcatcg cgttggccgt gtccagcagc agggtttcgc
     2701 gctccgggtc catcttggcc agttcgccaa agtgatcacg cagcaggatt gccgcagctt
     2761 ccacgtccgg cgcatccatc accgcatcgc gcagcggctt tgcatccttg ccgccgtagc
     2821 gcagcatgtc cgcccgcgcc atcgtgtcca ggctgcggtc ggtcgcccgg cgaaactcgc
     2881 ggtaaagctc gatctgcttg tcgctcaggt tgaacaagct tcgcagttcc gcatccttcc
     2941 acacaatgcc cggctgcaaa aaggcgccct cgtagctggt gttgatgttc ttctcgtact
     3001 gatccaccgg caggccgagc cacatcttgt gcacgttgcc cgagatcgca tcggcgccca
     3061 ttagcctgtc ggccttctgt cggatgctca tcttctcggc agcttccgac atatccgcaa
     3121 ccttcaccgc gcgcccggaa gcgtcgcggc cccacggcag cgtgccctcg aagaccggct
     3181 tcgcaatcgc cacgttgtcg gccgcgctga tcggcgactt ccagatgtcg cgccaggcgt
     3241 ccagcttcgg caacagcttc ggcgccatct cggccgcgtc gttggcgtag tggctcacgt
     3301 cgtcgatgaa gccctgggcg gccttgaaga ccggcgcaaa ggcgggcgag cgttcggcga
     3361 ggttgtacat ggtgccgatg ctacggtgcc accaggatag ctttccgggc gccgtgaagg
     3421 tgtcgttcag cgccgacgtg gccgcttttg tcgcggcgcg cagggtggcg gagcgggaga
     3481 agcggatgtc ggggctcgca gggtcgaagg ttccggcgtt gccggtggcg gatttgatct
     3541 ggtcggggga gaaaactatg aagacgtcag acgcctccgt tacctctgcg ttccacatat
     3601 tcgcagaatc gtaagtgttc ttgattatcg ctccgtcttt ccctgcgttc tttgcatctc
     3661 ctacaaaatc ataaaatttc cagtcacggt acaatttccc gtcttgatct gttatctctg
     3721 ggtccaaaat acgaacgtac actggataca caaccccacc gggctggatg ttcgcatctg
     3781 caagactggt ctctgctgcc ttgtaggcgc gcattgcttc cagcgcgttt tcgcgattgg
     3841 cttgttttgc ttcagactct gaggaatcgc gctcatcgga attttcccac gtataagact
     3901 gatacccata cttggtaagt tctttttctg ctgcggccag ctcccgatta gcttttttgc
     3961 tgttttcgta tcggcggctc tcttcagcaa cagaaacatc gtttcctagg gcggatgcgt
     4021 atccgctcgc aatcttttca gatgaggtaa aaaagaatcc taggcgtgcg ctttgtgcac
     4081 cagtaagccg cccaaggt
//
LOCUS       DAOSYS010000297         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18060_length_4030_cov_4.829434, whole genome shotgun sequence.
ACCESSION   DAOSYS010000297 DAOSYS010000000
VERSION     DAOSYS010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_4030_cov_4.829434"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..2518
                     /locus_tag="PLU99_14120"
     CDS             413..2518
                     /locus_tag="PLU99_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18226.1"
                     /translation="MIRLIYLWQSAANPTFTTPVMDSYDYDDLARALLDGHQPHTPLL
                     WQPVFYPLFLAGVYKVAGGSILAARILQVALGALTCALTYMLGVRVIGRRAGILAGII
                     VALYGPLIFYDADLTATGWAAFCAVAFVLLFIRCAAGFQPVSDSRGPGSQPASASCKT
                     SFRPVSLPASLALGACAALSILARPTFLPVVIAGALWLVYRACRNAKAGFSRLQTPKT
                     KGSGEADPYVTGRSSLKVLAGGLAGFLTITVPLAACSYPRTGHSGLLPASGGINFYIG
                     NNRNAAETIAARPGRDWRALEDLPARHGVHGDMWKQQRFYYAETARDVWHAPLGFVRG
                     LAVKAVEFFTSRELPRSEDIYVFRRWSPLLSALLWKIGGFGFPFGVLLPLALIGLIRH
                     GRRIPAPFWFMLILYPAAIILVFNAARYRAPLIPIIAVIAAGGALTIAEALRNLRWTA
                     LAGYGAICALVVAASTLPGPFPMEQLNYDAELHLLLGNRYNHEQKLAPAIEQFKAGLQ
                     FDSNNPDLHANLAKALLALERLDEAIEHYAAAVRLRPGSALNHTLYGLALFQRGDFER
                     AIEEYRRALEIEPGYGKVWSQLGVALEQSGRLDEAIDAYRKGVHASPNEVIIHYNLGA
                     ALAKRGQLESALNALQAALAVDANYIEARYELALVLQRLGRNAEAAAALQDVLARQPD
                     HAAARRLLSQIRGETPRRP"
     gene            complement(2573..>4030)
                     /locus_tag="PLU99_14125"
     CDS             complement(2573..>4030)
                     /locus_tag="PLU99_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18227.1"
                     /translation="DQVYFVDETVVLRPFERYTVPLWVRPDQPALRCTLTYADPAALP
                     SSSQHRINDLSLRVISPTGTVYWGNNGLLEGNWSVPDGEANTIDTVENVFVPHPEPGF
                     WTVQVIASEINEDGHVETPELDVDFALVVTGVAHEPLGISLPEGVPAYVAPGEPTSII
                     VRIGEGMETYVPGSGLLHYSFDGGDFETRPLNDLGSGLYEAVLPPAVCSATPRFYFSA
                     TGSGGTTVYNPPNAPDATYTADVGVLIIAFEDDFEQDRGWTVWNDPSLTGGAWERGAP
                     VVSGVTGDPPRADYDGSGKCYLTQNLPGNSDVDGGPTRLTSPPIDLTGLPDPVLEFAR
                     WWANDDQDGDPYDVEASSDDGETWVLLRRIQNVQPEWVREAIHLADFIPLTSQVRLRF
                     SVRDTPNNSIDEGGLDAVLVSAFNCTDVIVLGDVNCDGSINVFDIDPFVLALVDPAAY
                     ATAHPGCRIDNADCNGDGDVNVFDIDPFVALLIGG"
BASE COUNT          700 a         1330 c         1234 g          766 t
ORIGIN      
        1 ggagacgtcg ccccgcgggt gacacgcgcg gccggtgcca tgcgccgggt ctcgggtgcc
       61 acgggcgtcg ggtgccatgc gcgtcgggtg ccatgcccaa gcccgcgcag cgggcgcggg
      121 catgccctta ccggttgccg tgcgacaggt ctttggggcg gggcatgccc gcgcttacag
      181 ctttggcatt gcacccaact tggcgcccac cgaggtggca cccggccgga tgcactggcc
      241 gggccgcgta ctggtcgggc cggacgtaag ccgttagttt tccatcatgc acagcacccg
      301 ccgggcccgc acagatgacg ccaggctgaa caaacccgcc cgtctcaccg aagccggttc
      361 cacccgctgg cgtagccggc tctggccgtt ggccattttt ggcgcggcgc tgatgattcg
      421 cctgatctac ctttggcaat cggcggccaa cccgacgttc accacgccgg tgatggattc
      481 gtacgactac gacgacctgg cgcgggcgct gctcgatggc caccagccgc acacgccgct
      541 gctctggcag ccggtgttct atccgttgtt tctggcgggc gtttacaagg ttgcgggcgg
      601 gtcgattctg gcggcgcgca ttcttcaggt cgccctcggg gcactcactt gcgccctcac
      661 gtacatgctg ggcgtgcgag taatcggccg gcgggccggc attctcgctg gcatcatcgt
      721 cgccctttac ggcccgctga ttttctacga cgccgacctg acggcgaccg gctgggccgc
      781 cttctgtgcc gtagcgttcg tgctgctttt tatccgttgt gcggcgggct tccaaccagt
      841 cagtgactct cgcgggcccg gttctcaacc agcgagtgcc tcttgtaaga ccagttttcg
      901 accggtcagt ctgccggcgt ccctggccct gggggcctgc gccgcattga gcatcctagc
      961 gcgtccgacg ttcctgccgg tcgtgatcgc cggcgcgctc tggctcgtat acagagcttg
     1021 tcgcaacgcg aaagcgggtt tcagccgatt acagacgccg aaaacaaagg ggagcggcga
     1081 ggccgatccg tacgtaactg gccgaagctc gctgaaggtg ttagccggcg gtctggccgg
     1141 gtttcttaca atcacggttc cgctggcggc ctgcagctat ccgcgcaccg gccacagcgg
     1201 cttgttgccg gcttccggtg ggatcaactt ctacattggc aataatcgga acgccgccga
     1261 gacgattgcc gcccgcccgg ggcgcgactg gcgggccttg gaggatttgc cggcccggca
     1321 cggcgtacac ggggatatgt ggaaacaaca acggttctac tacgccgaga ccgcccgcga
     1381 tgtttggcac gcgccgcttg gattcgtgcg cggtctggca gtgaaagctg tggagttctt
     1441 cacctcgcgc gaactgccgc gcagcgagga catatacgtc ttccgccgct ggtctccact
     1501 gctcagcgcc ttgctctgga agatcggcgg atttggcttc ccgttcggcg tgctgctgcc
     1561 actggcactg atcgggctca tccgccacgg tcgccgaatc cccgcgcctt tttggttcat
     1621 gctcattcta tatccggcgg caatcattct cgtcttcaat gcagcgcgct atcgcgcacc
     1681 gctcatcccc ataatcgcag tgatagcggc cggcggcgct ttgacaattg ctgaggcact
     1741 ccgcaatttg agatggactg cacttgccgg ctacggcgct atttgtgcgc tggtagttgc
     1801 ggcttctact cttcccggcc cgtttccgat ggagcaactc aactatgacg ccgaactcca
     1861 cctgctgctc ggcaatcgct acaaccatga acagaagctc gcgcctgcca tcgaacagtt
     1921 caaggccggc ctgcaattcg actccaataa tcccgatcta cacgccaacc tcgccaaggc
     1981 actgcttgcg ctcgaacggc tggatgaggc catcgaacat tatgccgcgg ccgtccgctt
     2041 gcggcccggc tcggccctca accatacgct ctatgggctg gcactttttc agcgcggcga
     2101 cttcgagcgc gccatcgagg aataccggcg cgccttggag attgagcctg gctacggaaa
     2161 agtgtggtcg cagctcggtg tggcgcttga acaatccggc cggctggatg aggccatcga
     2221 cgcgtaccgc aagggcgtgc acgcctcccc gaacgaagtc atcattcact acaacctcgg
     2281 cgcggccctg gccaaacgcg gccaactgga atccgctttg aacgcgttgc aggccgcgct
     2341 ggccgtggac gccaactaca tcgaggcacg ctacgaacta gcgctcgtcc tgcagcgcct
     2401 ggggcgcaac gcggaggcgg ccgcggcgct gcaggacgtg ctggcccgac aacctgacca
     2461 cgcggccgca cgccggctgc tgagccagat caggggagag acgccgcgcc ggccgtagtt
     2521 gctccggcgc ggcagtagct cgcccggcgg ccgctccagc acaccgcttg cctcagccgc
     2581 caataagtag cgcgacaaac ggatctatgt cgaagacatt cacgtctccg tccccattgc
     2641 agtctgcatt atcaatgcga cagccgggat gcgccgtggc gtaggcggcc gggtcaacca
     2701 gcgcgagcac gaacggatca atgtcaaaaa cgttgatcga accgtcgcaa ttcacgtcgc
     2761 ccaagacgat tacgtcggtg cagttgaagg cgctgaccag aacggcgtcc agtccgcctt
     2821 catcaatcga attgttcggc gtgtcgcgca cactgaagcg cagtctgacc tggctggtca
     2881 gcgggatgaa gtcggccagg tgaatggctt cgcgcaccca ttcaggttga acattctgaa
     2941 tgcggcgcag caggacccaa gtctcgccat cgtcgctcga ggcctcgacg tcgtacggat
     3001 caccgtcctg gtcatcgttc gcccaccagc gcgcgaattc cagcaccgga tcgggcagac
     3061 ccgtcaggtc aataggcggc gaggtcagac gggtcgggcc gccgtcgacg tctgaatttc
     3121 cgggcaagtt ctgcgtcaaa tagcacttgc ccgagccgtc gtaatccgcc ctcggcgggt
     3181 cgcctgtaac gcccgaaacc accggcgcac cgcgttccca cgcgccgccg gtcagcgagg
     3241 ggtcgttcca aaccgtccag ccgcggtcct gctcgaaatc atcctcaaat gcaatgatta
     3301 ggacacccac gtccgccgtg tacgttgcat ccggtgcgtt cggcgggttg tagacggtgg
     3361 ttccgccact gcccgtggcg ctgaagtaga aacgcggggt agcgctgcat acggccggcg
     3421 gcaggacggc ctcgtacagc ccggagccca ggtcgttcaa cggtcgggtc tcgaaatccc
     3481 cgccgtcgaa actgtagtgc agcaatccag aaccagggac gtacgtttcc atgccttccc
     3541 cgatgcggac aatgatcgaa gtcggctcgc ccggcgcgac ataggccggc acgccttccg
     3601 gcaagctgat cccaagtggc tcgtgcgcaa ctccagtgac taccagggca aaatcaacgt
     3661 ccagttcggg cgtctcgacg tggccgtcct cattgatttc actggcgatt acttggaccg
     3721 tccagaagcc cggctccgga tgggggacga agacgttctc gaccgtgtcg atggtgttcg
     3781 cttccccatc aggcaccgac cagttgcctt ccagcaagcc gttattaccc cagtaaactg
     3841 tgcccgtggg cgaaataacc cgcaatgaaa ggtcattgat tctatgttgc gaggacgacg
     3901 gcagggcggc cgggtccgcg tacgtcagag tacagcgcag tgccggctga tccggccgga
     3961 cccacagcgg caccgtgtac ctctcgaacg gcctcagcac cactgtttcg tccacgaagt
     4021 acacttggtc
//
LOCUS       DAOSYS010000298         4029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18068_length_4029_cov_4.509059, whole genome shotgun sequence.
ACCESSION   DAOSYS010000298 DAOSYS010000000
VERSION     DAOSYS010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18068_length_4029_cov_4.509059"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLU99_14130"
     CDS             <1..1103
                     /locus_tag="PLU99_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOO18228.1"
                     /translation="VKLDQQEIGRSIANRVVLVTGAGGSIGSEMCRQIARFQPQRLVL
                     VEQAEQHLFEVGRELERSFPGLQFLPYIADITDKRRVEYVFDRERPSVVYHAAAHKHV
                     PMMEHNPGEAIKNNIHGTRVVADAACRFGCEMFVLISTDKAVNPTSVMGCSKRIAEMY
                     IQGLNGRSDTKFVTVRFGNVLGSSGSVIPIFREQIARGGPVTVTHPDMVRYFMTIPEA
                     SQLVLQAAAMSKGGTIFVLDMGEPVKIVDLAREMITLSGLRPDEDIKIEFTGVRPGEK
                     LYEELMIEGEGVSRTQHPKIGIWQSKPVNFESLRASIDEIISRADTLSRDEIRAWMKE
                     IVPEYRLNGSPEGPPAPIPSVAEVKAANGAAV"
     gene            complement(1138..1899)
                     /locus_tag="PLU99_14135"
     CDS             complement(1138..1899)
                     /locus_tag="PLU99_14135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOO18229.1"
                     /translation="MHEQRLILTDAPRAVLERIEPRVKLFCLAVWVICVVSIPPGDYR
                     ALAAYGLLLVALLATNVRLAGKFLRRFAPAVPVIMAAAVLLPFFVEGEVQWRLGPLVA
                     SRPGVNAGLRLAAVAGLCVAAVALVWASTRQELLLQGLRGVGLPSIFVNVLAFMLRYL
                     EVLRPELHRLTDARTARTISGRRPDRLRHSANLVGVLFLRAHDRAERVADAMAARGYT
                     GTLHTFHRPHFHARDLVVGAAFTGVVVGWRIVTMW"
     gene            complement(1871..2935)
                     /locus_tag="PLU99_14140"
     CDS             complement(1871..2935)
                     /locus_tag="PLU99_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOO18230.1"
                     /translation="MNAAPVLQPSYLLALHAPDGLFSLRVNLLFAALVALALVSAARL
                     LARRGEHRLPPVMGIMAAFIFAAQMANFPVAAGVSGHLLGGTLAAILLGPWAATLIMG
                     VVIFFQALLGDGGLTALGPNIFNMGIVGTFGGYLIFLAVRAGSQQPSRVVAAGAFAAW
                     CAVSLASVAISIQLALSGTAGLQRVLPAMLGIHMLIGVGEALITAAALAFVLKTRPDL
                     VYGLSSSRAPVCGSITRFGLAASVLACLALGLAAGPVPAYLSAPDGFEYVGRQQGFLP
                     EEFEAPESETPESEPTELSVAATSLAGGLGTLAMFAGSLLVGHVLTRRSRLERALEGS
                     TSTTNHEHRSRHARAASDSD"
     gene            3137..3811
                     /gene="elbB"
                     /locus_tag="PLU99_14145"
     CDS             3137..3811
                     /gene="elbB"
                     /locus_tag="PLU99_14145"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOO18231.1"
                     /translation="MTNSRVPKVGVLLSGCGFLDGAEIHEAVFTLLVLDQHQAEIVCC
                     APNTPQATTVDHVAGQPVAETRNVLTEAARIARGRIRDVATVSAGELDALILPGGFGA
                     AKTLCNFAERGGACDVHPAVEKLVGDMLAQRKPVGAICIAPALLGRVAAKRGLKPRLT
                     IGNDAGTAAALRQMGCIHDERGVTEIAVDEENKLVTTPAYMLGPGPAAVFEGIRKLVE
                     QVLRMI"
BASE COUNT          804 a         1281 c         1324 g          620 t
ORIGIN      
        1 ccgtcaagct ggatcagcag gagatcgggc ggtcgattgc caaccgcgtg gtgctggtaa
       61 ccggcgcggg cggctcgatc ggttcggaaa tgtgccgcca gatagcccgc ttccaaccgc
      121 agcggctggt gttggtggag caagccgaac aacacctgtt cgaggtcggg cgcgagttgg
      181 aacgcagctt ccccggcctg caatttctgc cgtacatcgc ggacataacc gacaagcgcc
      241 gcgtggaata cgtgttcgac cgtgagcggc cgagcgtcgt ctatcacgcg gccgcccaca
      301 agcacgtgcc catgatggag cacaatccgg gcgaggcgat caagaacaac atccatggca
      361 cgcgcgtcgt ggcggacgcc gcctgccgct tcggctgcga gatgttcgtt ctgatttcga
      421 cggacaaggc ggtcaatccg accagcgtca tgggctgcag caagcgcatc gcggagatgt
      481 acatccaggg cctcaacgga cgcagcgaca ccaagttcgt cacggtgcgg ttcgggaacg
      541 tcctgggcag cagcggcagc gtcatcccca tcttccgcga acagatcgcg cgcggcggcc
      601 cggtcaccgt gacccatccc gacatggtgc gctacttcat gacgattccg gaagcctccc
      661 agttggtgct gcaggccgcc gccatgtcaa agggtggaac gatcttcgtt ctggacatgg
      721 gcgagccggt gaagatcgtc gacctggcgc gcgagatgat cacgctttcg gggttgcggc
      781 ctgacgaaga catcaagatc gaattcaccg gcgtgcgccc cggcgaaaag ctctacgagg
      841 agctgatgat cgagggcgaa ggcgtctcgc gcacacagca ccccaagatc ggcatctggc
      901 agagcaagcc ggtgaacttc gagtcgttgc gtgccagcat tgacgagatc atcagccggg
      961 ccgataccct ctcacgcgat gagatccgcg cttggatgaa ggaaatcgtt ccggagtacc
     1021 gcttgaacgg ctcgcccgag ggtccaccgg cgcccatccc atcagtggcg gaggtgaaag
     1081 cggccaacgg cgcggcggtc tgagcacacc gctcggaatg cagcctgcgc gattacatca
     1141 ccacatcgta acgatgcgcc atccaaccac gacacccgtg aacgcagcac cgacgacgag
     1201 gtcgcgggcg tgaaaatgcg gcctgtggaa cgtgtgcaac gttcccgtat agccgcgcgc
     1261 ggccatcgca tccgcaacgc gctcggcacg atcgtgggcg cgcaaaaaca gcacgccgac
     1321 caggttggcg ctgtgacgca gacgatccgg ccggcgaccg ctgatcgtgc gtgcggtgcg
     1381 cgcgtccgtc aggcggtgca gctccggccg caggacttcc aggtagcgca gcatgaaagc
     1441 cagcacgttc acgaatatcg acggcagacc tacgccacgc aggccttgca gaagcagttc
     1501 ctgacgcgtg ctggcccaaa ccagcgccac cgccgccacg cacaggccgg ctacggccgc
     1561 aaggcgcaag ccggcgttga ccccgggccg gctggcaacc agcggcccga gccgccattg
     1621 cacttcacct tccacgaaaa acggcaacaa caccgcggcg gccatgatca ccggcacggc
     1681 cggcgcgaag cgccgcagga atttgccggc aagccgcaca tttgtcgcca gcagcgcgac
     1741 cagcagcaga ccataggctg ccaatgcgcg atagtcgccg ggcggaatgc tgaccacgca
     1801 aatcacccag accgccaggc agaatagctt gacgcgcggc tcgatgcgct cgagtacggc
     1861 gcgcggcgcg tcagtcagaa tcagacgctg ctcgtgcatg gcggctgcgg tgctcgtggt
     1921 tcgtggtgga cgttgatccc tcgagggcgc gttcgagccg gctgcgacgc gtgagtacgt
     1981 ggccgaccag cagtgagccg gcgaacattg ccaacgtgcc gagcccgcca gcgaggctgg
     2041 tcgcggcgac gctcagttca gtgggctcgg attcgggggt ctcggattcg ggggcttcaa
     2101 attcctcggg cagaaagcct tgctgccgcc cgacatattc gaaaccgtcc ggcgcactca
     2161 agtaagccgg caccggcccg gccgccagtc cgagcgccag gcatgcaagc acgctcgcgg
     2221 caaggccgaa ccgcgtgatc gacccgcaaa cgggggcgcg cgagctggac aaaccgtaga
     2281 ccagatccgg ccgcgttttg agcacgaatg ccaaggcggc ggcggtgatc agcgcttcgc
     2341 ccacaccgat cagcatgtga atgccaagca tagccgggag cacgcgctgc aagccggctg
     2401 tgccggaaag cgccaactgg atgctgattg cgacgctggc gaggctgaca gcgcaccagg
     2461 ccgcgaacgc cccggcggcg acgacgcgcg acggctgctg cgatccggct cgcaccgcca
     2521 gaaagatgag atatccgcca aacgtcccca ctatgcccat gttgaagatg ttgggcccga
     2581 gagctgtcag gccgccatcg cccagcaggg cctggaagaa gatgaccacg cccatgatca
     2641 gcgtcgcggc ccatggtccg agcagtatgg cggccagcgt accgcccagc aggtgcccgc
     2701 tgacaccggc cgcgaccggg aaattcgcca tttgagccgc aaagatgaag gccgccatga
     2761 ttcccatgac cggcggcagg cgatgctcgc cgcgccgcgc caacaagcgc gctgcgctaa
     2821 ccagggccag cgcgaccaag gcggcgaaca gcaggttcac gcgcaggctg aagagcccat
     2881 ccggcgcatg cagcgccagc aggtaagatg gctgcaaaac gggtgcggcg ttcatgtttg
     2941 cgatgctccg aggaaccgac ggtattacga ttataccggc agtattacca gtcaggcaaa
     3001 ccgttccgtt gcggcgggtc tcagaatcaa acccgggttg accgccggca agttcccggt
     3061 taccagtgcg ccgacggctg gggaggagct tcaccctccc gtgtcgcccc gaatcgatcc
     3121 ggaacaggag ttagtaatga ccaattcacg cgtgcccaaa gtcggcgtac tgctgtccgg
     3181 ctgcggattt cttgacggcg ccgaaatcca cgaggcggta tttactcttt tggtcctcga
     3241 tcaacaccag gcggaaatcg tgtgttgcgc tcccaatacg ccgcaggcga ccacggtcga
     3301 tcatgtcgcg ggccagccag tggccgaaac gcgcaacgta ctgacggaag cggcgcgtat
     3361 cgcgcgcggc cgtattcgcg acgtggcaac cgtttcagcc ggcgagctgg acgcgttgat
     3421 tttgcccggg ggcttcgggg ccgcgaaaac tctgtgcaac ttcgcggaac gcggcggggc
     3481 ctgcgacgtt catccggccg tggagaagct ggtcggcgac atgctggcgc agcgcaagcc
     3541 ggttggggcg atctgcatcg cgccggctct gctggggcgt gtcgccgcta agcgcggcct
     3601 aaagccacgg ctgacgatcg gcaacgacgc cggcacggcg gcagcgctgc gccagatggg
     3661 ctgcattcac gatgagcgag gagtaaccga gatcgcggtc gacgaagaga acaagctggt
     3721 gacgacgccg gcgtacatgc tcggcccggg gccggcggcg gtgttcgagg gtatacggaa
     3781 gctggtggag caggtgctgc ggatgatttg aagtccggtg cgtcgctaag ttgagcggga
     3841 cgggacgcac tgtttaaacg tactgttccc aagcttgaac ccgcactgct tgaagctgcg
     3901 ctttcaagca gtggcacacc gctttcagat cacccagtcc gcaccgtcga tggaggcggt
     3961 gcgagcgggt tggattttga cggacagttc caggcccgag ccgatgtcca cgagcatgct
     4021 gcacaaatt
//
LOCUS       DAOSYS010000299         4017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18132_length_4017_cov_5.302373, whole genome shotgun sequence.
ACCESSION   DAOSYS010000299 DAOSYS010000000
VERSION     DAOSYS010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18132_length_4017_cov_5.302373"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLU99_14150"
     CDS             complement(<1..833)
                     /locus_tag="PLU99_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOO18232.1"
                     /translation="MPVKPEMIRWACDRAAQDIAALAKRFPGLPQWLSGTARPTLRQL
                     ERFAKATHAPLGYFFLPRPPVEDVPIPDFRTMHRGAVRRPSPDLLDTIYICQQRQEWY
                     REHARITGERPFPFVGSATIDDDVEKTAAKMRDALAFDLEERRQMSTWQGALRRFIEQ
                     AENLGVLVMVNGVVGSNNLRRLDPEEFRGFALSDPLAPLVFINGADTKAAQMFTLAHE
                     LAHLWLGASALTDVQLRSAPTNETENWCNRVAAELLAPQALVRRELRGNEPLRETVDR
                     LA"
     gene            1467..3284
                     /gene="typA"
                     /locus_tag="PLU99_14155"
     CDS             1467..3284
                     /gene="typA"
                     /locus_tag="PLU99_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOO18233.1"
                     /translation="MAADYPNIRNVAIIAHVDHGKTTLVDQLLRQSGQFRAGELKGER
                     ILDSNAQERERGITILAKNIAISYRGTKINLIDTPGHADFGGEVERVLKMADGCLLLV
                     DAFEGPMPQTTFVLRKALEYGLRPIVVINKIDRPGARPREVHDEVLDLFIELGVSEDL
                     LDFVTVYSSATEGYATLDPLQRTNNVEALFEAIIKHVPPPTGDPAAPLQLLITALDYN
                     DYVGRIGIGRVFNGTLTAGRTVAVLHRDGTREDQRSTELHVFDGLERRKVDCVTAGDI
                     CAVVGLPAVDIGDTLADPENAKPLPLIAIDEPTLHMTFRVNDSPFAGRAGRFLTSRHI
                     WERLLREKQHNVALRISPGQTPEEFHVAGRGLLHLSVLIEDMRREGYELAVGKPKVIY
                     REQDGHKTEPIELCAIDVPAEHVGRVMEQMGGRRGVCRKMEARAERTYMEFLIPSRGL
                     IGLRNRLLNATSGTIIMHHNFYEYGLLRGEIPGRLNGVIIANESGQVTAYALDQLKDR
                     GEFFVGPTEPVYAGQVIGEHCREGDLGVNICRGKKLTNIRAASADKTVVLKPPRVMTL
                     EMALEFIEADELVEVTPAAIRLRKRLLNEVERKRAARAG"
     gene            complement(3340..>4017)
                     /locus_tag="PLU99_14160"
     CDS             complement(3340..>4017)
                     /locus_tag="PLU99_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOO18234.1"
                     /translation="NILGEAAPGRSSGEAMQAMEELVARLPQGVGHDWSGVSYQERMA
                     KSQTGLLYAFSMIVIFLCLAALYESWTVPISIMLVLPLGLIGGVVASSWRGLPNDVYF
                     QIGLLTVLGLTTKNAILIVQFALLKIEQGADLVQATLEGAKLRFRPIVMTSLAFGFGV
                     LPLALATGAGAGAQAAIGTSVLGGMATATFLAIFFIPLLFVLVSKVFGRKARRTAVAA
                     PLACETV"
BASE COUNT          789 a         1394 c         1186 g          648 t
ORIGIN      
        1 gccaggcggt ccaccgtttc gcgcagcggc tcgttgccgc gaagctcacg tctgactagc
       61 gcttggggtg cgagcagctc cgccgcaacg cgattgcacc aattctcggt ctcgttcgtt
      121 ggcgcggagc gtagttgaac gtctgtgagc gcggaggcgc ccagccacag gtgcgccagc
      181 tcgtgcgcta gcgtgaacat ctgcgccgcc ttcgtgtcag cgccgttaat gaacacgagc
      241 ggcgcgaggg ggtcagaaag cgcgaaaccc cgaaattctt cagggtctaa ccgacgcaga
      301 ttgttgctgc cgactacccc gttgaccatc acgagaacgc cgagattttc ggcttgttcg
      361 atgaaccggc gcagggcccc ctgccaggtg ctcatctgcc ggcgttcctc aagatcgaac
      421 gccaacgcat cgcgcatctt ggccgcagtc ttctccacgt cgtcgtcgat tgtggccgag
      481 cctacgaatg gaaacggccg ctcacccgtg atgcgggcgt gctcgcggta ccactcttgg
      541 cgttgctggc aaatatatat cgtgtcgagg aggtcagggc tggggcgcct cacagccccg
      601 cggtgcatcg tgcggaaatc cggaatgggg acatcctcaa ccggcggcct gggcaggaag
      661 aaatacccga gcggcgcgtg agtggccttc gcgaaacgct cgagttgccg gagcgtcggg
      721 cgtgcggtcc cgctcagcca ctgcggcagc ccggggaaac gtttggccag cgcggcgata
      781 tcctgcgcgg cccggtcgca ggcccatctg atcatctcgg gtttgacagg cactcgcgtc
      841 aatctctgct ccggctggcg agctgaaagt ctcgggcagc tccgaacgcg ccgtgatctg
      901 agcccgaagc gccggcgaag gcaatagctc gccggcacct tgccgccgct tgtcgatatc
      961 gaccatctta agtttaccgc agatcaccgc ggctgcacgc accgcggtag caccgcactg
     1021 cagtcagcta cagacgcctc agccgccggc gcgacgattc cgtaccactg gtgcgacgac
     1081 cgtcccgcac cttttcgcgc gggaatccgt cccgcccacc tactctccac tctccatctc
     1141 ctactcgccg cacgttcaat caagctcaag ggcaatgccg gctggatgtg gtccactgag
     1201 ccgcgcccgg cgcagcccac tgatgccccc ggcgtgccca aggtgcctcc tccctgccaa
     1261 agattcgaaa cctgacacca aaacccaatt cgcccggctt ctccccaccg ctgccttttc
     1321 ctctgttcat gcgttcattt cgcgggccgg tttcgcatat aatggttgga ttccgacacc
     1381 cgcgccggga caggcgcgcg gattaaacga agcgacactg gaaacgcacc gcctcaacga
     1441 cgccgcgagc gaggaaaata ccaaggatgg cagccgatta ccccaacatc cgaaatgtcg
     1501 ccatcatcgc acacgtcgac cacggtaaaa caaccctggt cgaccagctc ctgcgccaaa
     1561 gcggccagtt ccgcgccggc gaactcaaag gcgagcgcat cctcgattcc aacgcccagg
     1621 aacgcgaacg cggcatcacc atcctggcca agaacatcgc catcagctac cgcggcacca
     1681 aaatcaacct catcgatacg cccggccacg ccgacttcgg cggcgaagtc gaacgcgtgc
     1741 tgaaaatggc cgacggctgc ctcctgctgg tcgacgcttt cgaaggcccg atgccccaga
     1801 ccactttcgt cctgcgcaaa gccctcgagt acggcctgcg cccgatcgtg gtcatcaaca
     1861 agatcgaccg ccccggcgcc cgcccccgcg aggtgcacga cgaggtgctc gacctgttca
     1921 tcgaacttgg cgtgagcgaa gacctgctcg acttcgtgac cgtctatagc tccgccaccg
     1981 aaggctacgc cacgcttgac ccgctccagc ggaccaacaa cgtcgaggcc ctgttcgagg
     2041 ccattatcaa acacgttccg ccgccgaccg gcgaccctgc ggccccgctc cagctcctca
     2101 tcaccgccct ggactacaac gattacgtcg gccgcatcgg catcggccgc gtcttcaacg
     2161 gcaccctgac cgccgggcgc accgtggccg tgctgcaccg cgacggcacg cgcgaggacc
     2221 agcgcagcac cgagctgcac gtgttcgatg gcttggagcg gcgcaaggtc gactgcgtca
     2281 ccgccggcga tatctgcgcc gtggtgggac tgccggccgt cgacatcggc gacacgctgg
     2341 ccgatccgga gaacgctaaa ccactgccgc tgatcgcgat cgacgaacca accttacaca
     2401 tgaccttccg cgtcaacgat tcgccgttcg cgggccgggc cgggcgcttt ctcaccagcc
     2461 gccacatttg ggagcggcta ttgcgcgaaa agcagcacaa cgtggcgctg cgcatcagcc
     2521 ccgggcagac gccggaggaa ttccacgtcg ccggccgcgg actgttgcac ctttccgtgt
     2581 tgatcgaaga catgcggcgc gagggctacg agctggcggt tggcaaaccg aaggtcattt
     2641 accgcgaaca ggatggccat aagaccgagc cgatcgagct gtgcgcgatc gacgtaccgg
     2701 ccgaacacgt cggccgcgtc atggagcaga tgggcggccg ccgcggcgtg tgccggaaaa
     2761 tggaagcccg cgccgagcga acctacatgg agttcctcat tccctcgcgc ggcctgatcg
     2821 gcctgcgaaa tcgcctgctc aacgcgacca gcggcacgat catcatgcac cacaacttct
     2881 atgagtacgg gctgctgcgg ggcgagatac ccgggcggct caacggcgtc ataatcgcca
     2941 acgaaagcgg ccaggttacg gcctacgcgc tcgaccaact caaggaccgc ggcgaattct
     3001 tcgtcggccc gaccgaaccg gtctacgcgg gccaggtcat cggcgagcac tgccgcgagg
     3061 gcgacctggg cgtgaacatc tgccgcggca agaagctaac caacattcgc gccgcatcgg
     3121 ctgacaagac ggtcgtcttg aagccgccgc gcgtgatgac gctcgaaatg gcgctcgagt
     3181 tcatcgaggc cgacgaactg gtggaggtga cgccggccgc gattcgcctc cgcaagcggc
     3241 tcctgaacga agtggagcgc aagcgcgccg cccgcgcggg ataggcagcg tccgggctct
     3301 gctccaatcg tccctgccgg cgcaacggct ggatttgcgt cagacagtct cgcaggctaa
     3361 cggcgcagct actgcagtgc ggcgcgcctt ccgcccgaac accttggaca cgagtacgaa
     3421 cagcagcgga ataaagaaga tcgccagaaa ggtggctgtc gccatcccgc ccagcacgct
     3481 ggtgccgatg gccgcctggg cgccggctcc cgcgccagtc gccaatgcca aaggaagcac
     3541 gccaaagccg aatgccagcg aggtcatgac aatcggccgg aagcgcaact tcgcgccctc
     3601 aagcgtggcc tggaccaggt ccgcgccctg ctcgattttg agcagcgcga actgaacgat
     3661 caaaatcgcg tttttcgtcg tcagccccag cacggtcagc agcccgatct ggaaatacac
     3721 atcgttcggc agcccgcgcc aggaagaggc cacaacgccg ccaatcaggc ccagcggcag
     3781 aaccagcatg atcgaaatcg gtacggtcca gctctcgtac agcgccgcca ggcaaaggaa
     3841 tatcacgatc atcgagaatg catagagcag acccgtctgt gatttagcca tgcgctcctg
     3901 gtacgatacc ccactccagt cgtggcctac gccctgcggc aagcgcgcga cgagctcctc
     3961 catcgcttgc atcgcttcgc cggaactccg ccccggcgcc gcttcaccca agatatt
//
LOCUS       DAOSYS010000300         4010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18171_length_4010_cov_5.594690, whole genome shotgun sequence.
ACCESSION   DAOSYS010000300 DAOSYS010000000
VERSION     DAOSYS010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18171_length_4010_cov_5.594690"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLU99_14165"
     CDS             complement(<1..163)
                     /locus_tag="PLU99_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO18235.1"
                     /translation="MSETTWLIESAALSVRRGKQLVLRELSFRLAAGECVSLIGPNGS
                     GKSTLLLAML"
     gene            complement(156..1097)
                     /locus_tag="PLU99_14170"
     CDS             complement(156..1097)
                     /locus_tag="PLU99_14170"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOO18236.1"
                     /translation="MRAVALLSFLPLLIYGCTRDSARNAIPSAAVAEMHDWVRDPAVV
                     PSEQHSPPLRLLSLAPNATEICCALGLRDQLLARTRFCDYPPEIRNLPEVGDLAEINP
                     ETLVALRPDLVLVSGASRAITDKLVRLGLRFESLPDRNLEDVFAAVERVGRLTGRPLT
                     AAKLCDELKRELDQLAAKCAAVPRQRVLILLAPLSTPPAPPFAAGPGSFYDDLLRRAG
                     QANALAEEGRAFGPLALEAILRIDPDVIIELQPDGSDRPDGDADALRAWSRLGNLKAV
                     ANRRVQVLTGRQHYIPGPRVVQIFAELCRILGGEAHE"
     gene            complement(1094..1627)
                     /locus_tag="PLU99_14175"
     CDS             complement(1094..1627)
                     /locus_tag="PLU99_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18237.1"
                     /translation="MRIKLAPYGPAAALPLVPLLLIGCAPRRACVADAFPIASVAAPW
                     NLDGDVWSGTFEEAVPGLGADAGLWRDPQPTRVWLAIYRHENDSSRRLVARAFQFETA
                     AAAQTMYDRIRPQSANGFRAGDAGCWTSDGVLFVWGRLVFDVFAPQPGWQNELQAAML
                     AQHIQKRMPPGLPDTSP"
     gene            complement(1617..2303)
                     /locus_tag="PLU99_14180"
     CDS             complement(1617..2303)
                     /locus_tag="PLU99_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO18238.1"
                     /translation="MTQHGPLNIETFVEPMFQENGLLLWPQGQADCWFVDPGFPPQPE
                     ALAAAVHQHGLKPQAILLTHCHADHLAGVRLLQQKLPGTPLWAPRDEEHMLGDANANL
                     SAPFGFDVTAPPADRLLSPDEQLDLGGLSWTVLDVGGHSPGGLAYYCPAAGVVIVGDA
                     LFAGSIGRHDFPGSSQRRLLRNIRENLLTLPPETVVYSGHGPTTTVAREKRYNPFVGL
                     GAADEVDDED"
     gene            2401..3987
                     /locus_tag="PLU99_14185"
     CDS             2401..3987
                     /locus_tag="PLU99_14185"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOO18239.1"
                     /translation="MSPYLSTLAQATLPELDALRQYRDALISGQAFADWQSLWASFTG
                     FLHDLWTSGAVSDWRQGWMLVFSDRFLVVYFAPLLLLLLLLPQRHLRSGIIVTGLLFM
                     TYVFGAMYPLIWLAQCVIFYKLSEQFSIEVKRKDVLRWAPPTAAIACVGGWYIVSMML
                     TRIHFSGPTNAWMSENLTFLYPLGARVYPWESVWAWEPGKQPMQLFHALFFLPQVNGI
                     VIFTIRMMSYFSEIKRDTIPPAQRSLTKFLAFCTFAPTMIAGPIERYREFNHEIDHCR
                     ENRNWREMTYGLFRIALGLGKHLFNIVVFAPLVYGWVLNSGYYSHPENIRSYWALLIS
                     IQLQVFCLYLNFSGYSDVAIGMGRVMGYRVIENFDRYWLSCSLTEAWRRWHISFSFIL
                     RDYFFIPLVRRGWNVVLALMITFAVCGLMHNLAPSFVLWGLVMGGMVAINQRWSRWMR
                     TLDRQPHRALSAVRRHWMRLQPLPRLCAWFLTMNCLCGSALVIMNDRNIHGWRVIWEI
                     VRRPTAAVLNLFGVEWQGIP"
BASE COUNT          680 a         1218 c         1378 g          734 t
ORIGIN      
        1 cgagcatggc cagcagaagc gtgctcttgc cggagccgtt gggcccgatg agcgaaacgc
       61 actcaccggc cgccagacga aaactcagct cgcgcagaac gagctgtttt ccacgcctga
      121 cggaaagcgc cgcgctctcg attagccacg tggtctcact catgggcttc tccgccgaga
      181 atgcggcaca gctcagcgaa tatttgaacg acacgtggcc cgggaatgta gtgttgtctg
      241 ccggtgagga cctgaacccg gcgattggcg acggccttga ggttgcccag gcgtgaccag
      301 gcccgcagcg cgtcagcgtc gccatcggga cggtcggagc cgtccggctg cagctcaata
      361 atgacgtcgg ggtcaatcct taggatcgcc tcaagcgcga gcggtccgaa ggcacggccc
      421 tcttcagcca gggcgttggc ttgcccggcg cgccgcagca gatcgtcgta gaacgaaccc
      481 gggccggcgg cgaatggcgg cgccggcggg gtggaaagcg gggccaggag tatgaggacg
      541 cgctgccgcg gcacggcggc acatttcgcg gcaagctggt ccagctcgcg cttcagctcg
      601 tcgcatagtt tggccgctgt caaagggcgg ccggtgaggc ggccgacgcg ctcgaccgcc
      661 gcgaaaacgt cctcaaggtt gcgatccggc agcgattcga aacgcaggcc gagccggacc
      721 agcttgtcgg tgatggcccg gctggcgccg gacacgagca cgaggtccgg gcgcagcgct
      781 acaagtgttt cggggttgat ttcggccaga tctccaacct cgggcagatt tcggatttcc
      841 ggcgggtagt cgcagaagcg ggtacgcgcc aaaagctggt cgcgcagccc aagcgcacag
      901 cagatttcgg tggcgttcgg ggcgagggag agcagtcgca agggcgggct gtgctgctcc
      961 gaaggcacca ctgccggatc gcgcacccaa tcgtgcattt cggcgacagc ggccgaagga
     1021 atcgcgttgc gggcggaatc gcgcgtgcag ccgtagatta gcagcggcag aaatgatagc
     1081 agcgcgacgg ccctcatggc gaggtgtccg gcaatccggg cggcattcgc ttctgaatgt
     1141 gctgtgcgag catggcggcc tgaagctcgt tttgccagcc gggctgcggg gcgaagacgt
     1201 cgaaaacgag ccggccccag acgaagagca cgccgtcact ggtccaacag ccggcatcgc
     1261 ccgcgcgaaa accgttggcc gattgtggac gaatgcggtc gtacatggtc tgggccgcgg
     1321 cggcggtttc gaactggaat gcgcgggcca ccaggcggcg cgagctatcg ttctcgtggc
     1381 ggtagatcgc gagccagacg cgtgtgggtt gcgggtcacg ccacaggccg gcatcggcgc
     1441 cgaggccggg gactgcctcc tcgaatgtcc cgctccacac gtcgccgtcg agattccaag
     1501 gagcggcgac agaagcgatt gggaaagcat cggccacaca agcacgccgc ggcgcgcagc
     1561 caatcagcag caacggcaca agcggcaggg cggcggccgg tccgtatggt gcgagcttaa
     1621 tcctcatcgt cgacctcgtc cgcggcaccg agtccgacga acgggttgta gcgcttctca
     1681 cgggcaaccg tggtggtcgg gccatggccg gagtacacga cggtttcggg cggcaacgtg
     1741 agcaggttct cgcgaatgtt gcgcagcagc cggcgttgcg agctgcccgg gaaatcgtgg
     1801 cgcccaatgc tgccggcaaa cagggcgtcg ccgacgatga ccacgccggc cgccgggcag
     1861 taatacgcca ggccgccggg cgagtgcccg ccgacgtcca ggacggtcca gctcaagccg
     1921 ccgaggtcga gctgctcgtc cggggagagc aggcggtcgg ccggcggggc ggtaacgtcg
     1981 aagccgaacg gtgcggaaag attggcgttg gcgtcgccga gcatgtgctc ttcatcacgc
     2041 ggggcccaca gcggcgttcc gggcagcttc tgctggagca ggcgcacgcc ggccaggtgg
     2101 tccgcgtggc agtgggtgag caggatggcc tgcggcttga gaccgtgctg atggaccgcg
     2161 gccgccaggg cctcgggctg cggcgggaaa ccgggatcta cgaaccagca gtcggcctgg
     2221 ccctgcggcc agagcagcag gccgttctcc tggaacatgg gctcaacgaa agtctcgatg
     2281 ttaagcggtc cgtgctgggt catggcggga ttttagcgga agcgcggggg gatgtccgcg
     2341 cttggcgctt ggacaggact cccgctatgt tcacgcttgc cggggaaggc gagccagtgg
     2401 atgagcccgt atctgagcac gttggcgcag gctactttgc cggagttgga cgcactgcgg
     2461 cagtatcgcg acgcgcttat cagcggtcag gccttcgccg attggcagag cttgtgggcg
     2521 agcttcaccg gctttttgca cgatctgtgg acctcggggg cggtgtcgga ctggcgtcag
     2581 ggctggatgc tggtcttcag cgaccgcttc ctggtcgtct acttcgcgcc gctgctgctg
     2641 cttttgttgt tgctgcccca gcgacacctg cgcagcggga ttatcgtgac cggcctgctc
     2701 ttcatgacct atgtgttcgg ggcgatgtac ccgctgattt ggctggcgca gtgcgtcatc
     2761 ttctacaaat tgagcgagca gttttcgatc gaggtcaaac gcaaggacgt gctgcgctgg
     2821 gccccgccca cggcggccat agcgtgcgtc ggcggctggt acatcgtctc catgatgctg
     2881 acgcgcatcc acttctcggg cccgaccaac gcctggatga gcgagaacct gacgttcctt
     2941 tatccactgg gagcgcgggt gtatccgtgg gagtcggttt gggcctggga gcccgggaaa
     3001 cagcccatgc agctctttca tgcgctattt ttcctgccgc aggtcaacgg catcgtgatc
     3061 ttcacgattc gcatgatgag ctacttctcg gagatcaagc gggatacgat cccacccgcg
     3121 cagcgcagcc tgacgaagtt tctggcgttc tgcacgttcg cgccgacgat gatcgcgggg
     3181 cctattgagc gctaccgcga gttcaaccat gagatcgacc actgccgcga gaatcgcaac
     3241 tggcgcgaaa tgacctacgg gctgtttcgg atcgcgctcg gactgggcaa gcacctgttc
     3301 aacatcgtgg tcttcgcgcc gctcgtgtac ggctgggtgc tcaatagcgg gtactattct
     3361 caccccgaga acatccgcag ctactgggcc ctgctgatct cgatccagct ccaggtcttc
     3421 tgcctgtacc tgaacttcag cggctattcc gatgttgcga tcggcatggg acgcgtgatg
     3481 ggctatcgcg ttatcgagaa cttcgaccgc tactggctgt cgtgcagtct gacggaggcc
     3541 tggcgccgct ggcacatcag cttctccttc atcctgcggg attacttctt cataccgttg
     3601 gtgcggcgcg gctggaatgt cgtgctggcg ctcatgataa cattcgccgt gtgcgggctg
     3661 atgcacaacc tggccccgtc ttttgtgctc tggggcctcg tcatgggcgg catggttgcc
     3721 atcaatcagc gctggtcgcg ctggatgcgc accctcgacc gccagccgca ccgggcgcta
     3781 tccgcggtgc gccgccattg gatgcgcctg caaccgctgc cgcgcctgtg cgcctggttc
     3841 ctcactatga actgtctatg cggttcagcg ctggtgatta tgaacgatcg caacatccac
     3901 ggctggcgcg taatttggga aatcgtgcgg cgcccgacag cggcggtgct caacctgttc
     3961 ggcgtggagt ggcagggcat accgtagcgg ttgcacggcg gctgaagagg
//
LOCUS       DAOSYS010000301         4002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18210_length_4002_cov_5.513808, whole genome shotgun sequence.
ACCESSION   DAOSYS010000301 DAOSYS010000000
VERSION     DAOSYS010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18210_length_4002_cov_5.513808"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLU99_14190"
     CDS             <1..826
                     /locus_tag="PLU99_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOO18240.1"
                     /translation="IKAKYEYDPYGNLTRPLDQCGDYADDNPFRFSTKYWDDETGLGY
                     WGYRYYSPRMGRWISRDPLEEEGGINLYSYLDSQPLALIDPLGERQYSYEFNLRATGT
                     GFSFYMRGEFKDTEPCCAEVSVFAAGEWQPPFLHWVEKPFDLVNVHVEAGLRGGIKTT
                     AKLCMGMISSLKVCGRFEAFARAEYRHQRFKESGPKKRITRGRFGIGADGGVDLCWDL
                     CSGAVIIAGQVNAWAYVHFGWKWFNRSYSTSWEWASGETQIATVAAPLKKYCCLLN"
     gene            complement(1578..2651)
                     /locus_tag="PLU99_14195"
     CDS             complement(1578..2651)
                     /locus_tag="PLU99_14195"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOO18241.1"
                     /translation="MDRVRARRLHPYDRQKLHKMKRQLANQVNSRHARIILLSTGGVR
                     NRDIATLADCSPQWVRRIIHRFNAQGIDGITWYPFYADHTGQPRVFFADTVERIVEAA
                     LSPPRQLIGLNEWSLSKLRDYLIEQAVLRSISLEHLRQLLRQRGVRWRRTKTWKESTD
                     PEFIPKWRAIRRLYRRPPPNGRVICVDEFGPLNLQPRHGTCLARQGKVTRYRATYKRT
                     LGVRHFLAYYDLKTDRLYGYVSERKKTPDFLRFLKWIRQRYPQSQCLHMVMDNYGTHL
                     SHDVAAWARTHNVRLYFTPTNASWLNRIECHFGPLKKFALNSSDHRSHAEQEAAIMSY
                     LTWRNRRRAIGVQALTKARNHAA"
     gene            3034..3192
                     /locus_tag="PLU99_14200"
     CDS             3034..3192
                     /locus_tag="PLU99_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18242.1"
                     /translation="MCDEWVNLPEWRKTWGCVRGHWEKRWEIDAEGKILVNEWVCDEW
                     GWIDMGTM"
     gene            complement(3734..3901)
                     /locus_tag="PLU99_14205"
     CDS             complement(3734..3901)
                     /locus_tag="PLU99_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO18243.1"
                     /translation="MGKLIDDAPATTAILDRFLHNAEVITISGKSYRLRNRSPKEQPP
                     ATATACREHPK"
     gene            complement(3889..3957)
                     /locus_tag="PLU99_14210"
     CDS             complement(3889..3957)
                     /locus_tag="PLU99_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18244.1"
                     /translation="MRRHQLRSTIMTSNCSLEDWAS"
BASE COUNT          749 a         1091 c         1297 g          865 t
ORIGIN      
        1 catcaaggcc aagtacgagt acgatccata cgggaacctg acccggccgc tggaccagtg
       61 cggcgactac gccgacgaca acccgttccg cttcagcacg aagtactggg atgacgagac
      121 cgggctgggg tactggggct accgctacta cagcccccgc atgggccggt ggatcagccg
      181 ggatccgctg gaggaagagg gcggaatcaa cctgtacagt tatctagact cgcagccgct
      241 tgcgctgata gacccgttag gagagcgtca atactcctat gagtttaacc tgcgggcgac
      301 ggggacgggc ttcagtttct acatgcgcgg cgagttcaag gacactgagc cgtgttgcgc
      361 ggaagtttcc gtctttgccg ccggcgaatg gcagcctcct tttcttcact gggtcgagaa
      421 accatttgat ttggtgaacg tgcacgtaga agcgggcctg cgcggcggga tcaaaacgac
      481 tgctaagctg tgcatgggga tgatctcgtc gctaaaggtg tgtggccgat ttgaggcatt
      541 cgcacgcgca gagtatcggc accagaggtt caaggaaagc ggcccaaaaa aacgcattac
      601 tcgcgggcgc ttcggcattg gagctgacgg tggcgtcgat ctttgttggg atctgtgttc
      661 gggcgccgtg ataatcgctg gacaagttaa cgcctgggct tacgtccact tcggatggaa
      721 gtggtttaat cgatcctatt cgaccagttg ggaatgggcg agcggcgaga cgcagatcgc
      781 gaccgtggcg gctccgctga aaaagtactg ctgtttgctg aactgattgg gaaacgctat
      841 gcgaaacaga cgcggtacgg cacgagtgcc agaccgagat cgagccctgc cgtgcgacat
      901 cgagggattc caggttatgg cgtcgggcca ggacgtggta atcaggaaac gtgccgcgtc
      961 ggtcggagaa ctctgttttc tgctccctgc taccgtcgta actttcttaa tgggagttgg
     1021 cacggctgtc tgcgccgtta tcggcgtgct cagcacaatc cggggcagtt cccatccgtg
     1081 gtgggcgggc ctgctgatgg cctgcgtttt ggtcctcggg gcaggcattt gtggttgggg
     1141 attccagcgc ttgttgttgg tcatgctgcc ggtgacgcgc aggttagtct catgcgaggg
     1201 ctttgcagtt tgtgagttat cggtgaaagg ggtcagcctg cgacgggtgt ctctcaaata
     1261 cccgctgcag ttggacgccc gaccttacag cagccggggc gactggttgt gcgatttctc
     1321 gattcgagat gcggctggcc ggcggttcaa actgatgatc ccgaccgtca tatcggggtc
     1381 cagatctggc gcacagtcga ttgtgcgccc ctgcgctgag gcgatcgcaa gttgcatcgg
     1441 tgcacgggtg attgggctgg acgaaccgcc tgcccaggcc gggcgcaagt ctgggtaata
     1501 gccgatacaa gtgtacgatt tttaggcggg tggcgtctca gtcgagccag ccacccgcaa
     1561 gtacacctag tgccgtttca ggctgcatgg tttcgcgctt tcgttagggc ttgaacgcca
     1621 atcgcacgcc gccggttgcg ccaggtgaga tagctcatga tggcggcctc ctgttcggcg
     1681 tgactacggt ggtcagaact attcaacgca aacttcttga gcgggccgaa gtgacattcg
     1741 atgcggttca gccaggaggc gttcgtgggc gtgaagtaca gccgcacatt gtgtgtccgc
     1801 gcccacgcgg ccacgtcgtg gctgaggtgc gtgccgtaat tgtccatcac catgtgcaga
     1861 cactgcgatt gtgggtagcg ttgccggatc catttcagaa agcgcaggaa atccggcgtc
     1921 tttttgcgtt cgctcacgta cccgtacagc cgatccgtct tcaggtcgta ataggccagg
     1981 aagtgccgca cgcccagggt gcgtttgtac gtggcccggt agcgggtgac cttgccctgc
     2041 cgcgccaggc acgtcccgtg acgcggctga aggttcagcg gtccgaactc gtccacgcag
     2101 atcacgcgcc cattcggcgg tggccggcgg tacaagcggc ggatggcacg ccacttgggg
     2161 atgaactcag gatcggtcga ctctttccag gttttggtgc gtcgccagcg tacgccgcgc
     2221 tgccgcagca gctgtcgcag gtgctcgagg ctgatcgacc tcagcaccgc ttgttcgatg
     2281 aggtagtccc gcagcttgga gagcgaccac tcgttcaggc cgatcagttg cctgggcggc
     2341 gacagcgccg cttcgacgat ccgctcgacc gtatcggcga agaagacccg cggctggccg
     2401 gtgtggtcag catagaacgg gtaccaggtg atgccgtcga ttccctgcgc attgaagcgg
     2461 tgaatgatgc gtcgcaccca ctgcggcgag cagtccgcca gcgtggcgat gtcgcggttg
     2521 cgcacgccgc cggtggaaag caggatgatt cgcgcgtggc ggctattgac ttggttggcg
     2581 agttggcgtt tcatcttgtg cagcttctgc cggtcgtacg ggtgcagcct ccgtgctcgc
     2641 acacgatcca ttgtgaggtc tccacgcaaa aggtcctaga caaacccaaa cgtggagact
     2701 tcatttatcg ccgatcagga caacgcgcaa cccctttatc tataattagt tacatcaccc
     2761 gcgccaaaac gcgaaactct ggaagtggaa acggcactag cccgccaccc ctactcggcg
     2821 cgaccactcg gacctagttg ggggggcggc tcggagtgcg ctgggggaca gtggggaaat
     2881 aactgccaga ccagtacaat catccaactc ggaagctgct gcctcgagtc gaactggtcg
     2941 ccgaatgcat acgcgggcag cgctcgtggt tactgcaaac gcggccactg ggttacagtc
     3001 ggcgggtatg tttctccggc cacaaagaaa tgggtgtgtg acgagtgggt aaatctgcct
     3061 gagtggcgaa agacgtgggg ttgcgtgcgt ggccattggg agaaacgatg ggagatcgac
     3121 gcggaaggca agattctggt gaatgaatgg gtttgcgatg agtggggctg gatcgacatg
     3181 ggcaccatgt agaagagatg gagtgttcaa tgcggagccc gaacgtgcag ccgcttctct
     3241 ggttggcact acttaatatg cttctgatgc tcgcaacgcc gttggctgcc tcgggcctag
     3301 ccgcggtcgc ccatggcgtt gttacaagca attttcggga gctggattct aatggtgcca
     3361 tagaccacga cacggttctc cgagtctggg gtcccgaatg cagcaattgg catcgcgtag
     3421 tcgaccggct ggttgcggcg ccgctttcgt cggtttacca ggctggcttc atcgtatcgg
     3481 ggttttttgg tctaaatgcc gcatgcctgc tggttttctg gtggcgggta cgtgcgcaat
     3541 aaccgtacat cgcggccatt cagtgcacgg cggcggatag tcaggtgcaa ccgattcggg
     3601 ctgctgtccg gaatgcggca ccgcctttga cagtgcgcct gcccggaaca cctgacgctc
     3661 actgtcacct atcacttgaa ataccgccac caataatcac ttcaaatgca gccggaagcc
     3721 tcaaagatat ccgtcatttc gggtgctctc tgcaagctgt ggcggtcgca ggcggctgct
     3781 ctttggggct gcggttacgc aggcggtagc ttttgccgct gatggtgatc acctcggcgt
     3841 tgtgcaggaa gcggtccagg atcgccgtcg tggccggtgc gtcgtcgatc agcttgccca
     3901 atcctccagc gagcagttgc tggtcatgat cgtggagcgc agttggtggc ggcgcatgat
     3961 gatctcgaac aggtactcgc ccgaacgctt cggcagttgc tt
//
LOCUS       DAOSYS010000302         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18332_length_3979_cov_6.288226, whole genome shotgun sequence.
ACCESSION   DAOSYS010000302 DAOSYS010000000
VERSION     DAOSYS010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18332_length_3979_cov_6.288226"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLU99_14215"
     CDS             <1..786
                     /locus_tag="PLU99_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOO18245.1"
                     /translation="QIYLVLDAMTGQDAVNTAKSFDQRLELDGVILTKFDSDTRGGAA
                     LSVKHVTGKPIKFIGVGEKLDAIEEFHPDRIASRILGMGDVLSLVEKAQQQFDEKEAL
                     AQQQKMAKGTMGLDDFLVQLEKLRNMGPMKSILKMIPGVGSALNDLELPEEELNRFKG
                     VIHSMTPKERRNPELIEASRRRRIARGAGVQPQDVSGLVKQFIHMRPLMKAMAGQGIG
                     GRMKMMSQLSQMGMMDGAMPKLKKGTTRYNPQRDKKRKRRRRK"
     gene            903..1232
                     /locus_tag="PLU99_14220"
     CDS             903..1232
                     /locus_tag="PLU99_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18246.1"
                     /translation="MNWRTLSAFVMLFTLLTCPVFCLGDMGCADEEPAESEHDPGHAH
                     HPCLCKGAFPMTPSADLQRAIGLSAFVLPAAWPLIDQPADVCALLNASELLHNTLAFA
                     ALYPLLI"
     gene            1458..2891
                     /locus_tag="PLU99_14225"
     CDS             1458..2891
                     /locus_tag="PLU99_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOO18247.1"
                     /translation="MEAATGYCFEYRPENDACTQQRVCELLEGGLTADEAVHVCLLNN
                     RKIQAALYEIGAARADLVQAGLLSNPTLAIAGKFPDSGGLTKIEAAVVQNIADLWQIP
                     TRKRVACSALERVILQVARDASIAAYDAKAAYYQAKQADRKCELAAESIQVARHVLDL
                     ALTRQEAGAGSEIDVNLARAEVHSAELAFADAKLAALAARSALARQLSLPSAPEELEL
                     TDPLPELPHWCLDEEALLLLAADRLDIQAAASAVKAAVARIKEEKLKLFPNVEIGLAF
                     ERDERRAPEGRNILADSVLASIAERAPTVEIAPIERLSSDTLTGPSIGLELPIFNQNQ
                     GNIARAEYACQQNLKLLDDVVLAATQDVRLAAAQARAAWKVLDFYRDEVLPLRETSLD
                     LARTAYQAGRTPLHNVLETERALLTARVGYVDSLGDCAAALVELEKAVGQPLQRILPA
                     AGRAANEMDMDASPGTEAATQNSGEVP"
     gene            2888..>3979
                     /locus_tag="PLU99_14230"
     CDS             2888..>3979
                     /locus_tag="PLU99_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOO18248.1"
                     /translation="MKIGQILIGLILGAAVGAGGAWRLLNRAGEHDGHEHATHATAET
                     HDDHDHSAGQHDHDAHEDGCSHGHGHEHGEHDEGGAVVLSEKATREAGIKIVEAGPGE
                     LEETLTLPGEIGLNADKVAHIVPRVSGIVRRVEKSLGDEVQAGEVMAVLESRELAEAK
                     ADYLAARQRLALAEANLKSAQELHQKKIMPDLEFLTIEKDKAEAAIALQAAGNKLHAL
                     GLAEDQVPQSSDPTSSLALYELRAPFAGTVVQKHCALGEVLTSETDAFVLADLSTVWA
                     TISVYAQDIGRVRLSQTVHIRADGAETTASGQICYIAPVADEATRTVYARVDLPNTDR
                     QWRPGTFITASIVLDRKSVPVLVPIEAVQR"
BASE COUNT          803 a         1277 c         1247 g          652 t
ORIGIN      
        1 cagatttacc tcgttcttga cgccatgacc ggccaggacg cggtcaacac ggccaaatcg
       61 ttcgatcagc ggctggagct ggacggcgtg attctcacga agttcgactc cgacacgcgc
      121 ggtggagcgg ccctgtcggt caagcacgtg acaggcaagc cgatcaagtt catcggcgtc
      181 ggcgaaaagc tggacgcgat cgaagaattc caccccgacc gcatcgccag ccgcatcctc
      241 ggcatgggcg acgtgctcag cctggtagaa aaggcccagc agcaattcga cgagaaagag
      301 gccttggcgc agcagcagaa gatggccaag ggcacaatgg ggctggatga cttcctggtc
      361 cagctcgaaa agctgcgcaa catggggccg atgaagtcga tcctgaagat gatcccgggc
      421 gtcggctcgg cgctgaacga cttggaactc ccggaagagg agctgaatcg cttcaagggc
      481 gtgattcact ccatgacgcc caaggagcgc cgcaaccccg agcttatcga agcctcgcgc
      541 cgccggcgca tcgcgcgcgg cgccggggta cagccgcagg acgtcagcgg actggtcaag
      601 cagttcatac acatgcggcc actgatgaaa gcgatggccg gccagggcat cggcgggcgc
      661 atgaagatga tgtcgcagct ttcgcagatg ggcatgatgg acggggccat gccgaagctg
      721 aagaagggca cgaccaggta caacccgcag cgggacaaga agcgcaaacg ccgcaggcgg
      781 aagtgaattt ccagactcga ttggctagcc gggcgaagag cccggtaact tcgccagcgc
      841 gatggacgcg ctccggcggc ggggcttggc ggcagccccc cttgtcgcct aacattagga
      901 gcatgaactg gaggaccctc tcagcgttcg tgatgctctt cacgctgctg acgtgccccg
      961 tgttctgtct aggggacatg ggttgcgctg acgaagagcc ggcggaatcg gaacacgatc
     1021 caggacacgc tcatcacccc tgcctgtgca agggcgcgtt tcccatgact ccttccgccg
     1081 atctccagcg tgcgataggt ctttccgcct tcgtcctgcc cgccgcttgg ccactgatag
     1141 accagccggc cgatgtctgt gctctgctga acgcgagcga gctgctccac aatacacttg
     1201 cattcgcggc gctttatccc ctattgattt gatgcgctcc gaccgtttcc tcgattgagg
     1261 gcgccgcgcc ctcacggtta atcctgcgca cggccctcga ggccgccgac gcaccgtgga
     1321 cgccaaaact actcaggctg gaggattgag gtttgaagca ccgattgatt cgagcttcac
     1381 tgataacact tgcggcgact gcttccggtt gcgcaaccgt caatccgcga cccgactatg
     1441 atcgagccgc cgaaaaagtg gaagccgcga ccgggtactg ctttgagtac cggccggaga
     1501 acgacgcctg cactcagcag cgggtgtgtg agctgctgga aggcggcctg accgccgatg
     1561 aggcagtcca cgtctgcctt ctgaacaacc gcaagattca agctgcgctg tacgagatcg
     1621 gcgcggcccg ggccgacctg gtccaggccg gtctgttgtc caacccgact ctcgcgatcg
     1681 ccggcaagtt ccccgattcc ggcggtctga cgaagattga agcggcggtc gtgcagaaca
     1741 tcgcggattt gtggcagata cccacgcgca aacgcgtcgc ctgcagcgcg cttgagcgcg
     1801 tcattctcca ggtcgcgcgc gatgcgagta tcgccgctta cgacgctaaa gccgcctact
     1861 accaggccaa gcaggccgac cgcaaatgcg aactggcggc cgaaagcatc caggtcgcgc
     1921 ggcacgtcct ggacctggcc ctgacccgcc aagaggccgg cgcgggcagc gagattgatg
     1981 tgaacctggc gcgggccgag gtgcacagtg cggagctggc cttcgcggat gccaagctcg
     2041 cggcgctcgc ggcccgctcg gctttggctc gccagctctc gcttcccagc gcaccggagg
     2101 aactggagct gaccgatcca ttgcccgagc tgccgcactg gtgccttgat gaagaggcgc
     2161 tgctgctcct cgcggctgac cggctggaca ttcaagccgc cgccagcgcc gtcaaggccg
     2221 ccgtggcgcg catcaaggag gagaaactta agctcttccc aaatgttgaa attgggctgg
     2281 ccttcgaacg cgacgaacgc cgggcgcccg aaggccgcaa tatactcgcc gacagcgtac
     2341 tggcctcaat cgccgagcgg gcgcctacgg tcgagatcgc gcctatcgag cgtctctctt
     2401 cggacacact taccggcccg agcatcggct tggaactgcc gatcttcaac cagaaccagg
     2461 gcaacatcgc gcgggccgaa tacgcgtgtc agcagaacct caaattgctt gatgatgtag
     2521 tcctggccgc gacacaggac gtgcgcctgg ccgcggcgca ggcccgcgcc gcgtggaagg
     2581 tgctcgactt ttatcgcgac gaagtactcc cgctgcgaga aacaagcctc gacctggctc
     2641 gcacggctta ccaggccggc cgaactccgc tgcacaacgt gctggaaacg gagcgcgccc
     2701 tgctgacggc acgcgtgggt tacgtcgatt cgctagggga ctgtgcggcg gccctggtcg
     2761 aactggaaaa agcagtcggt cagccccttc agcgaatttt gccggcggcg gggcgagccg
     2821 ccaatgaaat ggacatggac gcgtcgccgg gtactgaagc ggcgactcaa aattcaggtg
     2881 aagtaccatg aagatcggtc aaattctcat cggacttatt ctcggcgccg cagtcggcgc
     2941 cggcggggcc tggcgcttgc tgaaccgcgc cggagaacac gacggccacg agcatgcgac
     3001 gcacgccaca gccgaaaccc acgacgatca cgaccactcc gccggtcaac acgaccatga
     3061 cgcccatgag gacggctgtt cgcacgggca tggccatgag cacggcgaac acgacgaggg
     3121 gggcgccgtc gttctgagcg agaaggccac ccgcgaggcg ggcatcaaaa tcgttgaagc
     3181 cggcccgggc gaactcgaag agacactaac cctgccgggc gaaatcgggc tcaacgccga
     3241 taaggtcgcg cacatcgtgc cgcgcgtcag cggaatcgtc cgccgcgttg agaaaagcct
     3301 cggcgatgaa gtgcaagccg gcgaagtgat ggccgttctc gaaagccgcg agttggcgga
     3361 agccaaagcg gattatctcg ccgcccgcca gcgcctggcg ctggccgaag ccaatctcaa
     3421 gagcgcgcag gaattacacc agaagaagat catgcccgat ctggaattcc tgacgatcga
     3481 aaaggacaag gcggaggccg ccatcgcact tcaagcggcg ggcaataagc tgcatgcgct
     3541 cgggctggcg gaggatcagg tcccgcagtc gtccgatccg acctccagcc tcgccttgta
     3601 cgagctgcgc gccccgttcg ccggcacggt cgtccagaag cactgtgcct tgggcgaagt
     3661 gctgaccagc gaaaccgacg cctttgtgct ggccgacctt tccacggtct gggccacaat
     3721 ttccgtctat gcccaagaca tcggccgcgt gcgcctgtcc cagaccgttc acatccgcgc
     3781 cgacggcgcg gagacgacag cctcgggcca gatttgctac attgctccag ttgccgacga
     3841 ggctacgcgt accgtctatg cccgggtcga cttgcccaat accgaccgcc agtggcggcc
     3901 cggcactttc ataacggctt cgatcgtact tgaccgcaaa tccgtgccgg tgctcgtacc
     3961 catcgaagca gtgcagcgt
//
LOCUS       DAOSYS010000303         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18507_length_3950_cov_5.395635, whole genome shotgun sequence.
ACCESSION   DAOSYS010000303 DAOSYS010000000
VERSION     DAOSYS010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18507_length_3950_cov_5.395635"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="PLU99_14235"
     CDS             <1..1665
                     /locus_tag="PLU99_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18249.1"
                     /translation="GEYVKETGGGPASQFAAALVGAFGGAGAAAGVSKSAAAIESGLK
                     RLLSPKMSTTDVNVVLSAVLSENGVKLSQVPGEIRAQLAEEMRQAMNTGKQLNPDVVR
                     RLADYAVVGATPTRGNVTLDPSLITQERNLAKVGANSSDKKLQALADLRNKNNARLIS
                     NLDEMGAAGRYAADPLAAGDAVARTILSKDAAMQGAAKALYDKARDASGKHIPLDREQ
                     FVNDAYRNLAESGKGAFLPPGILEKLQSIRVGKTSIDGRQFDVPFDAGVIDNLETLLA
                     TASRGATDGNARGAIKAVRDALNNVQPVAVGRQFGGGQAVPAGVLDGMQAQADESATA
                     ALNAFREARAAHRARMEWQESTPAIRAILDDAVSSENLIKQFFLSSGNRSGAKQTAEL
                     INQIKGNPQAKQAMREAAMEFIKSKALGGAESEVGVLSQKGLNTALRQLGDYKLKMLF
                     TPDEIAALKANARVASYEQVQPAGSAVNNSNTTGAAAGLLERLANMPLVSNIPYAGPL
                     IQQGSLRIRTNQAMTPIEAMAQEAAKPKQQYLPLSAILAIPALQNR"
     gene            1775..2389
                     /locus_tag="PLU99_14240"
     CDS             1775..2389
                     /locus_tag="PLU99_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18250.1"
                     /translation="MAGLSPFKPQFFDNNGDPLVGGKLYAYAAGTTTPQDTYTDAGAT
                     AANTNPVILNSRGEADIWLTPGVHYDFVLKSASDVLIWTLPSVRSYGVQAQESSDGFF
                     GWVNSAIGQIWQFVPTPAINALPKNVRWVLRCLSSGDGAFTAGDYIDITHNSVGGASY
                     QIVGGAIKFSYSQNGVLQVKDKAVAAANVTLTAARWDLKCIAEF"
     gene            2393..2629
                     /locus_tag="PLU99_14245"
     CDS             2393..2629
                     /locus_tag="PLU99_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18251.1"
                     /translation="MNAYAGPDRREHVQLTDEQIDQIVTRAADKAAVRAVEMLTQAAY
                     QEVGRKAVGFTSWIAGMGILALLGWLIAHDKIKF"
     gene            2632..3051
                     /locus_tag="PLU99_14250"
     CDS             2632..3051
                     /locus_tag="PLU99_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009670517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOO18252.1"
                     /translation="MDMTKRCIERVSKIHPDLRRVVYRAADLLGNTHADGLGFVVTEG
                     MRTAARQAELVKAGASQTMSSRHLTGHAVDIAATVGGKVRWDWPLYYKLANVFFEAAR
                     IENVPIEWGGDWPKLRDGPHYQLPHAMYPKPRGKSNA"
     gene            3044..3412
                     /locus_tag="PLU99_14255"
     CDS             3044..3412
                     /locus_tag="PLU99_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18253.1"
                     /translation="MRNSVAVILSVLAFSAQAQSTQTLDCTSKPCKAVASWTPADASL
                     TGCNLYITNGAGTTLPAISGTVTTPGAACTMNMPTLLKGAYTLNAKGVNAFGEGAAMA
                     VPLSLTTGTAPGAPSAVVVQ"
     gene            3489..3668
                     /locus_tag="PLU99_14260"
     CDS             3489..3668
                     /locus_tag="PLU99_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18254.1"
                     /translation="MLEGYKTLIGAIVALAAEGARLAGVDIGDQEGLVNSILVIFGGI
                     LAIYGRMKASGPIGG"
     gene            3694..>3950
                     /locus_tag="PLU99_14265"
     CDS             3694..>3950
                     /locus_tag="PLU99_14265"
                     /inference="COORDINATES: protein motif:HMM:NF018755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HOO18255.1"
                     /translation="MTQIAEAGEFMPAQFELDAPLVFDSRHLSHPVEVPTRFRTDLAS
                     VPRLPLVWMIAGGIGNQASVVHDYLYRERPDVARSVADAVFL"
BASE COUNT          799 a         1162 c         1304 g          685 t
ORIGIN      
        1 ggtgagtacg tcaaggaaac gggtggcgga ccggcctcgc aattcgctgc cgctcttgtc
       61 ggcgcattcg gtggcgcagg cgcggcggct ggcgtctcga agtccgctgc ggcgatcgag
      121 tccggcctaa agcgtcttct gtcgcccaag atgagcacga ccgacgtgaa tgtcgtctta
      181 agcgcggtgc tgtctgaaaa cggcgtcaag ctgtcgcagg tgccgggtga aatccgcgcg
      241 caacttgcgg aagaaatgcg gcaggcgatg aacaccggca agcagttgaa tccggatgtg
      301 gtgcgccgtc ttgcggacta tgccgttgtc ggggcaacgc caacgcgggg aaatgtgacg
      361 ctcgacccgt cattgattac gcaagagcgt aacctggcga aggtcggggc caacagctcc
      421 gacaagaagc tccaggcgct tgcagatctg cgcaacaaga acaacgcgcg gttgatttcc
      481 aaccttgacg agatgggcgc ggcggggcgc tatgccgcag acccacttgc ggccggcgat
      541 gccgttgcaa ggacaatcct gtccaaagac gcggcgatgc agggcgcggc aaaagcactt
      601 tacgacaagg cgcgcgatgc ctcgggcaag catattccat tagaccgcga acagttcgtc
      661 aacgacgcat accgcaacct tgcagaatcc ggcaagggcg cgtttctccc accaggcatt
      721 ctggaaaaac ttcaatccat ccgggtcgga aagaccagca ttgacggtcg tcagttcgac
      781 gtacctttcg atgccggcgt gattgataat ctggagaccc tgttggcgac ggcaagccgt
      841 ggcgcaacgg acgggaacgc acgaggcgcg atcaaggctg tccgcgacgc gctgaacaac
      901 gtgcagccgg tagcggtcgg gcgccagttt ggcggcggac aagccgtccc ggctggcgtg
      961 cttgacggca tgcaggccca agcggacgaa tccgcaacgg cggcgctaaa cgcattccgg
     1021 gaagcccgcg cggcgcatcg cgcccgcatg gagtggcagg agtccacgcc ggcaattcgc
     1081 gcaatactgg atgacgccgt gtcgtcggaa aacctcatca agcaattttt cctctcatct
     1141 ggcaatcgat cgggcgccaa gcagacggcg gagctgatta accagatcaa aggcaatccg
     1201 caggcaaagc aggccatgcg cgaggccgcg atggaattca tcaagtccaa ggcacttggc
     1261 ggcgcagagt ctgaggtcgg ggtactgtcg cagaaggggc tgaacaccgc gctacggcaa
     1321 ctcggcgatt acaagctcaa gatgctgttc acgccggacg aaatcgccgc gctgaaggcg
     1381 aacgcgcggg tggcgagtta cgagcaggta cagccggccg ggtcggcggt gaacaactcg
     1441 aatacgacgg gcgcggcggc ggggctgctt gagcgactgg cgaatatgcc gctagtctcg
     1501 aatatcccct atgcggggcc gctcattcag caggggtcgc tgcgcatccg gacaaaccag
     1561 gccatgacgc cgattgaagc gatggcgcag gaggcggcaa agccgaagca gcaatatttg
     1621 ccgctgtcgg caatcttggc aattcccgcg ctgcaaaacc gttagcggct gctaatccaa
     1681 tgccagagca ggacgccgca taacgttcct agcaatatcg gatcgatctc cattccagcc
     1741 gcctccgcgc ggctttttgc attgagggtc caacatggcc ggacttagcc cgttcaagcc
     1801 gcagttcttc gacaacaacg gcgacccgct ggtagggggg aaactctacg cctacgcggc
     1861 cgggacgaca accccgcagg acacgtatac ggacgctggc gcgacagctg ccaacaccaa
     1921 cccggttatt ctcaactcgc gcggcgaggc ggatatctgg ctgactccgg gggtgcatta
     1981 tgacttcgtg ctgaaaagcg caagcgacgt attgatctgg acgctgccat ctgtgcgcag
     2041 ttatggggtg caggcacagg aaagctcgga cggctttttc ggatgggtta attccgcgat
     2101 cggacagatt tggcaatttg tgccgacccc ggcaatcaac gcactgccga aaaatgtgcg
     2161 atgggtgttg cgctgcctgt catcgggcga tggcgctttc acggccggcg actacatcga
     2221 catcacgcac aacagcgtcg gcggcgcgag ctatcaaatc gtcggcggcg ccatcaagtt
     2281 cagctatagc cagaacggcg tgttgcaggt gaaggacaag gcggtggcgg cggcaaacgt
     2341 gacgctgacg gctgcgcgat gggatctgaa gtgcatcgcg gagttctgag ccatgaacgc
     2401 ctacgccggc ccggatcgcc gcgaacacgt gcagcttacc gacgagcaga tcgaccagat
     2461 cgtcacccgc gcagcagaca aagccgccgt gcgggcggtc gagatgctga cgcaagccgc
     2521 ctatcaggag gtcggcagaa aggcggtcgg attcacgtca tggattgccg gaatgggcat
     2581 cctggcgctg ctcggctggt tgatcgctca tgacaagatc aagttctagc catggacatg
     2641 accaagcgtt gcattgagcg cgtgtcaaag atccacccgg acctgcggcg cgtcgtgtat
     2701 cgcgcggctg acctgctcgg caacacgcac gccgacggcc tcggcttcgt cgtgaccgag
     2761 ggcatgcgca cggcggcgcg tcaggctgag ttggtaaagg ccggcgcctc gcagacgatg
     2821 agcagccggc acctgaccgg gcatgcggtg gatatcgcgg ccacggtcgg cggcaaggtg
     2881 cggtgggact ggccgctgta ctacaaactg gcgaatgtgt tttttgaagc ggcgcgcatc
     2941 gaaaacgtgc cgatcgaatg gggcggcgac tggcccaagc tgcgggacgg cccgcactac
     3001 cagttgccgc atgcgatgta tccgaaacct cgggggaaga gcaatgcgta attctgtcgc
     3061 tgtaatcctg tccgtgcttg cgttctctgc gcaagcgcaa tccacgcaga cgttggactg
     3121 caccagcaag ccctgcaagg cggtggcaag ctggacgccg gccgatgcct cgctgaccgg
     3181 ctgcaatctc tacatcacga acggcgcggg gaccacgctg ccggctatca gcggcaccgt
     3241 gacgacgcct ggcgctgcct gcacgatgaa catgccgacg ctgttgaagg gcgcgtacac
     3301 actcaatgcc aagggcgtca atgcgttcgg ggagggcgct gcgatggcgg tccctttgtc
     3361 actgacgacg gggacggcgc cgggcgcgcc gtccgccgtt gtagtgcagt agagaagcgc
     3421 ctgcagggct tcagctgcta cctgctggac ctgctgctta atcgcggctt tttgaaggag
     3481 atccgataat gcttgaaggc tacaaaaccc tgatcggcgc catcgtcgcg ctggcagcgg
     3541 aaggcgcgcg actggctggc gtcgatatcg gagaccagga agggctggtc aattccatcc
     3601 tcgtgatctt cggcggcatc ctcgccattt acggccgcat gaaggccagc gggccgatag
     3661 ggggctgacg tggggtttcg cacgccgctg agcatgacgc agatcgccga ggccggcgag
     3721 ttcatgccgg cgcagttcga gcttgacgcg ccgctggtgt tcgatagtcg gcatctatcg
     3781 catccggtcg aggtgccgac gcggttccgc accgatcttg ccagcgtgcc gcgcctgccg
     3841 ctggtgtgga tgattgccgg cggcatcggc aaccaggcgt ccgtggtgca tgattacctg
     3901 taccgcgagc ggccggacgt ggcgcgatcg gtggcggatg ccgtgtttct
//
LOCUS       DAOSYS010000304         3941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18548_length_3941_cov_4.212815, whole genome shotgun sequence.
ACCESSION   DAOSYS010000304 DAOSYS010000000
VERSION     DAOSYS010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_3941_cov_4.212815"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="PLU99_14270"
     CDS             <1..709
                     /locus_tag="PLU99_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18256.1"
                     /translation="APLRGLGHGICALGVRRRGGARGGRQSGLYLCGRAAGAAEPDHA
                     ATPRASYVTMSARPGFGPSQGRFMLDWLPFQEPIVAKIVAAAGALLVLTIVVWFWQRR
                     REAALAARCRKELQRVHDQLQQQQREISELAAQIIATGSTAVIAGFQVVRQIDAIFTE
                     GQRSPAEAVEALKALAARQGANAIINLNSQRLPNGKCTASGDAVIVRPLAGNAPDVPA
                     AEIKRGNGEPRLDRRWD"
     gene            700..1749
                     /locus_tag="PLU99_14275"
     CDS             700..1749
                     /locus_tag="PLU99_14275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO18257.1"
                     /translation="MGLTNTGQPELACWEFAGLLLTYWCNARCAFCYVFGGPNRGGEM
                     PVERAVELWRGLDRHAAAHGKTMRVHLAGGEPFGDWPRLLAILQKAREAGLTPPDNIE
                     TNAFWATDDELTRARLKVLDQLGLRMLVVSADVFHQQFVPFERVRRCVETARTVLGPG
                     RVRVRWWDFYNAPLDLTRAGEAERRQAFDAALRRHQDRLTGRAALRLPQFLPRYPAAQ
                     FRGETCAKAILGSRHVHIDGHGNIFPGVCSGIILGNAVHATVQEVWQDVAQHWQDNPV
                     VAALVCGGSYELLRRAQAHGFQESPGGYANKCHLCACVRQFLFERHIWPEVIGPAECY
                     ADQAEQRAADASTGV"
     gene            1770..2639
                     /locus_tag="PLU99_14280"
     CDS             1770..2639
                     /locus_tag="PLU99_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOO18258.1"
                     /translation="MGIIDTTKLMDGRRLARTILAAAQESVQRIRSSTGVQPTLATVL
                     VGDDPASATYVRMKRKRCDEVGMASIAVELPAGISTEDLVEHIQRLGRDPAVHGILVQ
                     HPVPAPIDRRAVFEAIPLEKDVDGVTACTLGRAILGMPAFVPCTPAGIMRLMMEYKVQ
                     LKGVHAVVIGRSPILGRPMASLLINAHATVTLCHSHTRNLANIVAGADVVIAACGQPR
                     FVRGNWIKPGAVVIDAGYNEGVVGDVDFEPAAKRASLITPVPGGVGPMTIAVLIQHTA
                     EAAARQLDVDLND"
     gene            complement(2668..3633)
                     /locus_tag="PLU99_14285"
     CDS             complement(2668..3633)
                     /locus_tag="PLU99_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOO18259.1"
                     /translation="MVERFTLGIDLGGTNLKAGICDAQGSLVAQHAIETKAELGFKGV
                     LARMVALVDELVEMSGKRRTEISAIGVGAPGPLSRAAGLIYNAPNLPGWVNVPLRQRL
                     SDATGLPVVLENDANAAAFAELIAGAGRGAHSLVLLTLGTGVGGGIVLDGQVWHGADD
                     SAGEMGHTMLVPGGRPCPCGQAGCFERYCSANAIGVRFVEAIEAGEDSMLAAQVRAGG
                     EVDALAVQRAMEAGDALATRIWDETCYYLALGCITIERMLAPDLIVLAGGLIGAGDLL
                     LRPVQEYYEKLRWRLTKRVTGIAFSTLGGQAGIIGAAMVARTMPT"
BASE COUNT          687 a         1292 c         1314 g          648 t
ORIGIN      
        1 cgcgccgtta cgcggcttgg ggcatggcat ctgcgcgctg ggtgtgcggc ggcgcggcgg
       61 cgctaggggc ggtcgtcaaa gcgggctata cttgtgcggg cgcgcggcgg gcgccgcgga
      121 gccggaccat gcggccaccc cgagagcgtc ttacgtcacc atgagcgcaa ggccaggatt
      181 tggcccgagc caaggcaggt ttatgctgga ttggctgccg tttcaggaac ccatcgtggc
      241 aaagatcgtc gctgccgccg gcgcgctgct cgtgctgacg atcgtggtgt ggttctggca
      301 gcggcggcgt gaagcggcgc tggcggcacg ctgccggaag gaactgcaac gcgtccatga
      361 ccaacttcag cagcagcagc gcgaaatcag cgaactggcg gcgcagataa tcgcgaccgg
      421 ctcgaccgcc gtcatcgccg gcttccaggt cgtacgccag attgacgcca ttttcaccga
      481 aggccagcgt tcgccggccg aagccgtcga agccctcaaa gcactggccg cgcgccaggg
      541 cgccaacgcc atcatcaacc tcaacagtca gcgcctgccg aacggcaagt gcaccgccag
      601 cggtgacgcc gtgatcgtgc gtccgctagc gggcaacgcc ccggacgtgc cggccgcgga
      661 aatcaagcgt ggcaacggcg agccgcgcct ggatcgcaga tgggattgac taacacgggc
      721 caacccgaat tggcgtgctg ggagttcgcg gggctgctgc tcacgtactg gtgcaacgcg
      781 cgctgcgcct tctgctacgt tttcggcggg ccaaaccgcg gcggcgagat gccggtcgaa
      841 cgcgcggtgg agctgtggcg cgggctggac cgccacgccg ccgcccacgg caagaccatg
      901 cgcgtgcacc tggcgggagg cgagccgttc ggcgactggc cgcggctgct ggcaatactg
      961 cagaaggctc gcgaagccgg gctgacgccg ccggacaaca tcgaaaccaa tgccttctgg
     1021 gcgaccgatg acgagctgac gcgcgcccgg ctgaaagtgc tcgaccagtt ggggctgcgg
     1081 atgctggtcg tcagcgccga cgtttttcac cagcaattcg tgcccttcga gcgcgtgcgg
     1141 cgctgcgtgg aaaccgcccg caccgtgctc ggcccgggac gcgtccgcgt gcgctggtgg
     1201 gatttctaca acgccccgct ggacctcacg cgagcgggcg aggctgaaag acggcaagcg
     1261 ttcgacgcgg ccctgcgccg gcaccaggac cgcctgaccg ggcgggccgc actccggttg
     1321 ccccaattct tgccccgcta cccggcggcc cagttccgcg gcgaaacttg cgccaaggcc
     1381 atcctcggca gccggcacgt acacatcgac ggccacggca atatcttccc cggcgtgtgc
     1441 agcggaatca ttctgggcaa cgccgtccac gcgaccgtgc aagaggtctg gcaggacgtg
     1501 gcgcagcatt ggcaggataa tccggtcgtt gcggcgcttg tctgcggcgg aagctacgaa
     1561 ctgctgcgcc gcgcgcaggc ccacggcttc caggaatcgc ccggcggcta cgccaacaag
     1621 tgccacctgt gtgcctgcgt tcggcagttc ttgttcgaaa ggcatatctg gccggaggtc
     1681 attgggccgg cggagtgcta tgccgaccag gccgagcagc gggccgccga cgcctcgacc
     1741 ggcgtgtgac cgcgctgtat tctgccgcaa tgggcattat tgacacgacc aagctgatgg
     1801 atgggcggcg gttggcgcgc accattctgg ccgccgccca ggaaagcgtg cagcgcattc
     1861 gttccagcac cggcgtacag ccgaccctgg cgaccgtact ggtgggcgac gatccggcct
     1921 cggccactta cgtgcgcatg aagcgcaaac gttgcgacga ggtcggcatg gcctcgatcg
     1981 cggtcgagct gccggccggc atcagcacgg aggacctcgt cgagcatatc cagcgcctgg
     2041 gccgcgatcc ggccgttcac ggaatcctgg tgcagcaccc cgtgccggcc cccatcgacc
     2101 gccgggcggt attcgaggcc attccactgg aaaaggacgt tgacggcgtg acggcctgca
     2161 cgctgggccg cgccatcctg ggcatgccgg cgttcgtgcc ctgcacgccg gccggcatca
     2221 tgcgtctgat gatggagtac aaggtccagc tcaagggcgt acatgcggta gtcattgggc
     2281 gcagcccgat cctgggccgg ccgatggcgt cgttgctgat caacgcgcac gccacggtga
     2341 cgctgtgtca ttcgcacacg cgcaatctgg cgaacatagt cgccggggcg gacgtggtga
     2401 ttgccgcatg tgggcagccg cgcttcgtgc gaggaaactg gatcaagccc ggcgccgtag
     2461 tcatcgatgc cggttacaac gagggcgtgg tcggggatgt ggacttcgag ccggcggcga
     2521 agcgcgcgtc attgattaca ccggtgccgg gcggcgttgg gccgatgacg atcgcggtgc
     2581 tgatccagca caccgccgaa gcggccgcac ggcaactcga tgttgacctg aacgactgag
     2641 ccgtggcgtt ttgtcagtgc tcagtgctca agtgggcata gtccgggcga ccatcgccgc
     2701 tccgatgatg ccggcctggc cacctagcgt cgaaaacgcg attccagtca cgcgcttggt
     2761 caatctccag cgcagcttct cgtagtactc ctgcaccgga cgcagcaaca aatcgccggc
     2821 cccgatcagt ccgcctgcca gaacgatcaa gtccggcgcg agcatgcgct caatcgtgat
     2881 gcaacccagc gccaggtaat agcaggtttc atcccaaatt cgcgtagcca gcgcatcacc
     2941 cgcttccatc gcgcgttgaa cggccagagc gtccacttcg ccgccggctc tgacttgcgc
     3001 agcgagcatg gagtcttcgc cggcttcgat tgcttcgacg aagcgcaccc cgatcgcgtt
     3061 agccgagcag taacgctcga agcagccggc ttggccgcac gggcacggcc ggccgcccgg
     3121 gacgagcatg gtgtgtccca tctcgccggc cgaatcgtcg gcgccgtgcc acacctggcc
     3181 gtcgagtacg attcccccgc cgactcctgt tccgagcgtc aagagcacaa ggctgtgagc
     3241 gccgcggccc gcgccggcaa tgagttccgc gaaagccgcg gcgttcgcat cgttttcaag
     3301 gacgaccggg aggcctgtag cgtcgctcag gcgctggcgc aacggaacgt tcacccagcc
     3361 gggcaggttg ggcgcgttgt agatcaaacc cgcggcgcgc gagagcggcc ccggagcacc
     3421 cacgccgatg gctgagattt cagttcggcg cttgccgctc atctcgacca actcatcgac
     3481 gagcgcgacc atgcgcgcta gcacaccttt gaaccccaac tcagctttcg tttcgatggc
     3541 gtgctgtgcg acaagcgagc cctgcgcgtc acaaatgccg gccttcagat tggtgccgcc
     3601 caggtcaatc cccaaggtga agcgttcaac cataatgaac tctctccgcc gtaagacaaa
     3661 tttctagcga gtctcgggcc gctccgcgct cgcccgatta tgcacgcaat gcgagtacag
     3721 ttgcaaatgg caagcgcacg ccgttgatca ggcgcagacc gctagccggt cctttatcag
     3781 gtatgcggaa aaaaaggtcg gagcaggcgg cgacgaccgg ggtgttcggg ccgaagggag
     3841 gggggtcgcc gcttggggtg cctgctccgc tacggtcact cgtaattata gcgaagggcg
     3901 caggtggggc aattgaattc cgagccctga cgtaggtttt t
//
LOCUS       DAOSYS010000305         3933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18590_length_3933_cov_4.979371, whole genome shotgun sequence.
ACCESSION   DAOSYS010000305 DAOSYS010000000
VERSION     DAOSYS010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18590_length_3933_cov_4.979371"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLU99_14290"
     CDS             <1..454
                     /locus_tag="PLU99_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOO18260.1"
                     /translation="AGCELMLAADIVIASETARIGLPDTGVGAIPGAGGTQRLTRVAG
                     KALAMDMILTGRTLSADEALQAGLVSRVVPRENYLSEAMHVCHELSKRAPLALQLAKR
                     AVLSAYDSTLAAGLAHEHDLFQIVVGTADYREGLRASLEKRPAVFAGR"
     gene            558..899
                     /gene="xseB"
                     /locus_tag="PLU99_14295"
     CDS             558..899
                     /gene="xseB"
                     /locus_tag="PLU99_14295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOO18261.1"
                     /translation="MPRRVLREDLLMAAEHKASKLVKNLRFEEAMERLDAIVAAMEAG
                     QIGLEESLAKYEEATQLAARCREILDQAEQRIQKIQLDAAGRPQVTPFEPPQPSSGVE
                     PDEPCPPQPPE"
     gene            915..1769
                     /locus_tag="PLU99_14300"
     CDS             915..1769
                     /locus_tag="PLU99_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOO18262.1"
                     /translation="MAAIWTAVGSGLRGAWYGLADVLLPQTCITCGLWIPAGTGATCE
                     RCAQEIATLRQASYCPRCGRTMAVPAIHGKSCARCIHEPFWNIAGVARIGAYQGVLRP
                     LITQLKYRGHERNAVFAAELLAAAMQRAGWAADIAGLVPVPMSWLRRVQRPCDHAALL
                     ATALGKQLGLRVMPVVRRLKHRPSQTNLASRQSRFANVKDCFGPKRFGARSVGGKTIC
                     IVDNLITSGATVCELSKVLRRAGAKKIYAAVIARAVLLGDPDKPQPDPPIAEASVEAD
                     LPNQGVDV"
     gene            complement(1766..>2569)
                     /locus_tag="PLU99_14305"
     CDS             complement(1766..>2569)
                     /locus_tag="PLU99_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18263.1"
                     /translation="DEGTTVYQPGDAPTHVFSLRAVNLAVYLDDDFEADEGWTVTNDP
                     SLTSGAWQRGVPLSQPGAPEADYDGSGRCYVTDNRAGALDVDYGPTNLDSPLFDFSTA
                     SDPVICYARWIYCDDFMPPAQDFLDVQLSYDGGTTWVTAEHVSGAGGWERRELRVRDY
                     VSLTAQVQLRFSVGDNPNNSITEAAIDAVRVYDLWCPAGLPCDTNCDGAVNVFDIDPF
                     VLAMVDSAGYAAAYPMCSPTAADVNQDGEVNVFDIDPFVECLTRGGQGD"
     assembly_gap    2570..2669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2670..3439)
                     /locus_tag="PLU99_14310"
     CDS             complement(<2670..3439)
                     /locus_tag="PLU99_14310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOO18264.1"
                     /translation="MNLRCIQFSAALFGILMGVLPAALASAEGMAAADQVSLESYYQL
                     MDVHLYTHSGMSRSALSGAQHDAARDSILALFQSYGLSAHLETFSGYYSGENVVAEKL
                     GTLYPDQIFVFGAHYDSVNCPGANDNASGVAGVLEAARVLSQYPSDYTIRFIAFDQEE
                     QGLIGSEEYVDAHADDNFLGMISADMIAYDPGTDTCNIYGRTASNPIKASLAAAVGEY
                     GQGLTPIIGGDTPYSNHAPFESAGFQACLLIEGEVWSDP"
BASE COUNT          692 a         1157 c         1301 g          683 t
ORIGIN      
        1 cgccggctgc gagctgatgc tggcagcgga tatcgtcatc gcttcggaaa cggcccgtat
       61 cgggctgccg gacactggtg tgggcgcaat ccctggcgcg ggcggcacgc agcgcctgac
      121 gcgcgtggcg ggcaaggccc tggcaatgga catgattctc accggacgaa cgctgtcggc
      181 cgacgaggct ctgcaggccg ggctggtcag ccgcgtcgta ccgcgagaga actatctctc
      241 agaagccatg cacgtctgcc acgaactgag caaacgtgct ccactggcgc tgcaactggc
      301 caagcgcgcg gtgttgagcg cttatgattc gacgctggcg gcaggactag ctcacgagca
      361 cgatctcttc cagatcgtcg tcggcacggc ggactatcgt gagggtttgc gggcctcgtt
      421 ggagaagcgg cccgcggttt tcgccgggcg ctgagaatat cattagctcg gccgagccgg
      481 cgccagccgc gcggacttga ccgctagacc tgtgcggcgc gatcattcgc ggccaaggac
      541 tggttcggct gggcatcttg ccacggcgcg tactgcgtga ggacctgcta atggccgctg
      601 agcacaaggc gagcaagctc gtcaagaacc tgcgcttcga agaggcgatg gagcgcctgg
      661 acgcgatcgt ggccgccatg gaagcggggc agattggtct ggaagagtcg ctggcgaagt
      721 acgaggaagc cacgcaattg gcggcccgct gccgggagat actcgaccag gcggagcagc
      781 ggatacaaaa gattcagctc gacgcggccg gtcggcccca ggtgacgcca ttcgagcccc
      841 cgcaacccag cagcggggtt gagccggatg agccgtgccc gccccagccg ccggagtaag
      901 tcaaaggtca tgaaatggcg gcgatttgga ctgccgtagg ctccgggcta cgcggcgcgt
      961 ggtacgggtt ggcggatgtt ttgctgccgc agacctgcat aacctgcggc ctgtggattc
     1021 ccgcgggaac aggtgctact tgtgagcgat gtgcgcagga aatcgccacc ctgcgccagg
     1081 cgagctactg cccgcgctgc gggcgcacca tggcggtgcc cgcgattcac ggcaaatcct
     1141 gtgcgcgttg cattcacgag cccttctgga acatcgccgg cgtggcccgg atcggtgctt
     1201 accagggggt gttgcgtccg ctgatcactc agctcaagta ccgcggccac gagcgcaacg
     1261 ccgtgtttgc cgcagaactg ctggcagccg ccatgcagag ggcgggctgg gccgcagaca
     1321 tcgcagggct cgtgccggtg ccaatgagtt ggctgcggcg cgtgcagcgg ccgtgcgacc
     1381 atgcagcgct tttggcgaca gcattgggca agcaactggg cttgcgggtc atgccggtcg
     1441 ttcgccgact caagcacagg cccagtcaga cgaatctcgc ctcacgacag agccgcttcg
     1501 caaatgtgaa ggattgtttt ggcccgaagc ggttcggcgc gcggtcggtc gggggcaaga
     1561 cgatctgcat agtggataat ttgatcacca gcggcgcgac ggtctgtgaa ctatctaagg
     1621 tcctgcgccg cgccggcgcg aagaagattt acgcggccgt gatcgcgcgc gccgtgctgt
     1681 tgggagatcc cgacaagccg cagcctgacc cgcccatcgc cgaggcgtct gttgaggcgg
     1741 acctgccgaa tcagggcgtt gatgtttaat cgccctggcc gccccgcgtg aggcactcca
     1801 caaacgggtc gatgtcgaac acgtttacct cgccgtcttg gttgacgtct gccgccgtcg
     1861 ggctgcacat cgggtaggcc gcggcatagc cggccgagtc gaccatcgct aggacgaatg
     1921 ggtcgatatc gaagacgttg acggccccgt cgcagttcgt gtcgcaaggc aggcctgcgg
     1981 ggcaccacag atcgtacacg cgcaccgcgt caatggcggc ttcggtgatg gagttgttgg
     2041 ggttatcgcc gacggagaag cgcaactgca cttgcgccgt caggctgaca tagtcgcgca
     2101 cgcgcagctc gcggcgctcc cagccgcccg caccgctgac gtgctcggct gttacccaag
     2161 ttgtgccgcc gtcatatgaa agctgcacgt ccaaaaagtc ctgtgccggc ggcataaagt
     2221 catcacagta gatccagcga gcgtagcaga tgaccgggtc gctggcggtg ctgaaatcaa
     2281 agagcggcga gtccaggttc gtcggcccgt agtccacgtc caaagccccg gcgcgattgt
     2341 ccgtgacgta gcaccggccc gagccgtcgt aatcggcctc cggcgcgccg ggttggctga
     2401 gcggcacgcc gcgctgccac gctccgctgg tcaggctggg atcgttcgta accgtccagc
     2461 cttcatcggc ctcgaaatcg tcgtccagat aaaccgccag gttcacggcc cgcaggctga
     2521 agacgtgcgt cggggcgtcg ccgggctgat agaccgtagt gccttcgtcn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gatcgctcca gacctcgccc tcgatcagca
     2701 ggcaggcctg gaagccggcg ctctcgaacg gggcgtgatt gctgtagggc gtgtcgccac
     2761 cgatgatggg cgtcagaccc tggccgtact cgccgacggc cgccgccagc gacgccttga
     2821 tgggattgga cgcggtgcgg ccgtagatgt tgcaggtgtc ggtgcccggg tcgtaggcga
     2881 tcatgtcggc cgagatcatg cccaggaagt tatcgtcggc gtgcgcgtcc acgtattcct
     2941 cgctgccgat cagcccctgt tcctcctgat cgaaggcaat gaagcggatg gtgtagtcgc
     3001 tcgggtactg gctgaggacg cgtgcggcct ccagtacgcc ggccacgccg ctggcgttgt
     3061 cattggcgcc ggggcaattg accgaatcgt agtgcgcgcc gaagacgaag atttgatcgg
     3121 ggtatagcgt gccgagcttc tcggcgacga cattctcgcc ggagtaatag ccggagaacg
     3181 tctcaaggtg ggccgatagg ccataactct ggaagagggc caatatgcta tcgcgggccg
     3241 cgtcgtgctg cgcgccgctc aaagcgctgc ggctcatgcc gctatgggtg tacagatgca
     3301 catccatgag ctggtagtag ctctcgaggc tgacctggtc ggccgcggcc attccctcag
     3361 ccgaagccag agccgccggc aggacaccca tcagaattcc gaacagggcc gccgaaaact
     3421 ggatgcatcg tagattcatc tttcgccctc agtcaggttc agtagttcgc cagacgcggc
     3481 gcggcggcac aacagagcgc cggccgtagc gcgcacggtc gggcaggcct tcttctccag
     3541 atttgatcag tctagcagtt tttgcgtgag aaaggcaagg ctttaatggt tggcagtgcg
     3601 acgcaaggca accgccaaga tgccaagagc gctaagaccg ccaagaacga ggaatggcgg
     3661 cggtgcggga gggagtaagc gcaagctatc gattaggttt cattggcggt ctcacacatt
     3721 tggatgcccg ataatagtgg aaaaggcaac gaggcagtca gttggagact cgatctgagt
     3781 cgacggcgga agggtctatc ttgccgggca aattactgcc gcgctccggc cgcgatcagg
     3841 gcgccggggc ctttcttttc tctggcgggc ggccagaggc gcgtggggtc gtaccagtgg
     3901 tcgaggatgg ccgaccagct tcgttcatcg ccg
//
LOCUS       DAOSYS010000306         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18688_length_3918_cov_4.547243, whole genome shotgun sequence.
ACCESSION   DAOSYS010000306 DAOSYS010000000
VERSION     DAOSYS010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18688_length_3918_cov_4.547243"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1816
                     /locus_tag="PLU99_14315"
     CDS             383..1816
                     /locus_tag="PLU99_14315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOO18265.1"
                     /translation="MISPNPATPPASDVVLVSADNELRGIVRRQRPPGLRLTCFGPDD
                     APKTAAAHCGELWLDLDCPRAAEEAEKAAVCFLTAPPSENAGLMPRSGRALLVRKPCL
                     PDVAQRLWRAVTRQRLRVPRPAPTLPAWMCEFHQIGLRELCRLCVTRLPERLGYAAAS
                     LYLHDPDTGVLSLTETTHARPIDAAISLGSDRERLMADVARSGRLLRTSNAIKEWRAR
                     SLPAPRRSNYSDGACLIAPLMCDGRLWGLLNLSGRRAGGAQFSGPLEPIFDFLARSLR
                     YAREYERAHFEARVDVLTGLYNQRWLREALEKEIRRSERFSIPLSLIAADLDGLKAVN
                     DREGHPAGDCVLRHVARRISSVLRQFDSAARVGGDEYAILLPATAYEGGRQVARRILE
                     SIRGDAACYRGVPLPIRASLGVAQWEPGWDAARLIEAADQAMYLAKKQGRDQLICLEP
                     ATAQHCDAGVPPAVAQASGSAEDLVHK"
     gene            1995..2894
                     /gene="yaeI"
                     /locus_tag="PLU99_14320"
     CDS             1995..2894
                     /gene="yaeI"
                     /locus_tag="PLU99_14320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HOO18266.1"
                     /translation="MSDSSKQLTERITRRRLLAGGLAGLGTLTAGGIYALRIEPFWPA
                     LTRLPMDLPRLDPELAGFKLVQISDLHISAGKTISYLRKQIDFCNSLAPDLLVITGDL
                     VTRGDPRRMEDLAALVKPLRAKHGVFAVLGNHDYNRFQPARQPDPRAQRIANQIVDIL
                     QANGVQVLRNQCHPVTRGNARVQLVGLDDYWCDNYNPPAAFATVDPQLPCIALCHNPD
                     AIEDLRRLPCHWVLSGHTHGGQVRIPLLGAPLLPVRNRQYDAGLFHLGQQRLYVNRGL
                     GYLRRIRFNCRPEITEFTLHPLA"
     gene            3236..>3918
                     /locus_tag="PLU99_14325"
     CDS             3236..>3918
                     /locus_tag="PLU99_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOO18267.1"
                     /translation="MNVALFITCLTDSFFPRAGEAVVRVLRHFGCRVQFPAAQTCCGQ
                     PAYNSGFHDEARVLARRMIEIFEPYETIVSPSASCVAMVKQHFAPLLADEPAWRARAE
                     RLAERTHEFATFLVDRLNVDIAGLLRFDEPVTFHYPCHARGIYGAEDLQRWLTRDGQV
                     NLRLPQHVDCCCGFGGMFAVDYPEISTAMLNDKLDLLAATGARLVICNEGGCTLQMAG
                     GARRRGLPLR"
BASE COUNT          647 a         1404 c         1199 g          668 t
ORIGIN      
        1 cccgcttccg ccagccgcgt cacgtccacc agcagcgccg gcacatccgg ctggatcggc
       61 tccggtatca gccggtacag cgcgccggcc gccgcgaccg ccgcaaccag ggcgagcggc
      121 acttgccacc ggtcgctaag gacggccgcg atctgctgtt tgaacccgga gtcgctcatc
      181 ctgagctttc ctctgcatcc tgcggatggg gcgtgtggcc ccgacgctgc tccactgttc
      241 tatcggatgg aagtccgtcc gcagcgcagg aaattgcgcc cctgccgcgt gcaacacctg
      301 cgcctgcgaa ccacccgcgg ccggtcccgg gccgccccgt tccgcctcta gcgccgggcg
      361 ctgaaggtcc gatgtagtta tcgtgatatc tcccaatccg gccacaccgc ccgccagcga
      421 cgtggtgctc gtcagcgccg acaacgagct gcgcggcatt gtgcgccgcc agcgcccgcc
      481 cggactgcgt ctcacgtgct tcggaccgga cgacgcaccg aagactgcgg ccgcgcactg
      541 cggggaactg tggctggact tggattgccc ccgcgccgcc gaggaggctg aaaaagccgc
      601 ggtttgcttc cttaccgcgc ctccgtccga gaacgcgggg ctaatgcccc gcagcggccg
      661 tgcactcctg gtgcgcaaac cctgcctgcc ggacgtcgcg caacggctgt ggcgcgcggt
      721 tacacggcag agattgcggg ttccccgacc cgccccaacg cttccggcct ggatgtgcga
      781 gtttcaccag atcggcctgc gcgagctgtg tcggctgtgc gtcacgcgac tgccggagcg
      841 gctgggctac gccgcggcct cgctgtacct gcacgatcct gatacgggcg ttctctcact
      901 gacggagacg acccacgctc gtccaattga cgccgccatc agcctgggca gcgaccgcga
      961 gcgcctcatg gccgacgtgg cccgctcggg gcggctgctg cgaaccagca acgcgataaa
     1021 agagtggcga gcgcgctcac tgccggcgcc gcgccgctcc aattactcgg acggagcctg
     1081 cctgatcgcg ccactgatgt gcgacggccg gctctgggga ttgctgaatc tcagtggccg
     1141 gcgggcgggc ggtgcacagt tttcggggcc gctggagcca attttcgact ttctggcccg
     1201 ctcgttgcgt tacgcgcgcg agtacgagcg tgcccatttc gaagcccgcg tcgacgtgct
     1261 gaccgggctt tacaaccaac gctggctgcg cgaagcgctg gagaaggaaa tccgccgtag
     1321 cgagcgcttc agcattccgc tctcgctcat cgcggccgac ctggacggct tgaaagccgt
     1381 caacgaccgg gaaggccacc cggccggcga ctgcgtactg cggcacgtcg cgcggcgcat
     1441 cagttcggtg ctgcggcagt ttgattcggc cgcacgcgtc gggggtgacg aatacgccat
     1501 attactgccg gccacggcgt acgagggcgg gcggcaggtc gcgcggcgca tcctggagtc
     1561 catccgcggc gacgcggcct gttaccgcgg cgtgccgtta cccattcgcg ccagcttggg
     1621 cgtagcccaa tgggaaccgg gctgggacgc ggcgcgcttg atcgaagcgg ccgaccaggc
     1681 catgtacctg gcgaaaaaac agggtcgcga tcaactgatc tgcctggaac cggccaccgc
     1741 acaacactgc gacgcaggcg tcccaccagc agtggcgcaa gcttcaggca gtgccgagga
     1801 cttagttcac aagtgataag gtaatagagt gataaggtga taaaggacga tccgcggaac
     1861 ccctttatca ctctatcact ttatcactct gtcaccccgt tgcccgcgtc ccactgctcg
     1921 aaagcggcat caccagttga ttcgcacttc cagcgaacct aacctacgag ctgaaatagc
     1981 cggagatgcc gtcgttgtca gacagcagta aacagctcac agaacgcatc acccgccgcc
     2041 gtctgctggc cggcggcctc gcgggcctgg gaaccctgac cgcgggcggc atttacgccc
     2101 tacgaatcga acccttctgg ccggcgctca cccgcctgcc gatggacctg ccgcgactcg
     2161 accccgagct ggccggcttt aaactggtgc aaatctccga tcttcacatc tcagccggca
     2221 agacgatttc gtacctgagg aaacagatag acttctgcaa cagcttggcc cctgacctgc
     2281 tcgtcatcac cggcgacctg gtaactcgcg gcgatcccag gcgcatggag gacttggcgg
     2341 cgctcgtcaa accgctccgc gccaagcacg gcgtcttcgc ggtcctgggc aatcacgact
     2401 acaaccgctt ccagcccgcc cgccagcccg acccgcgcgc ccagcgcatc gccaaccaga
     2461 ttgtcgacat tctgcaggcc aacggcgtgc aggtcctgcg caatcaatgc caccccgtca
     2521 cccgtggcaa cgcccgcgtg cagctcgtcg gcctcgacga ctactggtgc gacaactaca
     2581 acccccctgc cgctttcgcc actgttgacc cgcagttgcc ttgcatcgct ctctgccaca
     2641 accccgacgc aatcgaggac ctgcggcgcc tgccctgcca ctgggtgctc agcggccaca
     2701 ctcacggcgg ccaggtacgc atcccactgc tcggggctcc cctgcttccc gtcaggaacc
     2761 ggcaatacga cgccggtctg ttccacctgg gccagcagcg cctctacgtc aaccgcggcc
     2821 tgggctacct gcgccgcatc cgtttcaact gccgccccga aatcaccgaa ttcacgctac
     2881 acccgctggc gtgagcccct ttcccgcgtt tggagtctgt agcgccgacc ccccgtggtc
     2941 gacgttccag gcccgtagcg ccgatccccc gtggtcgacg tcctcccaac gtcggccagg
     3001 ggcgaccgac gctaccacac cgttctgccc gatccccggt gtaccacgcc tgcggcgtca
     3061 gctccgcgtt tcagtactgc gtgattcacg cgatcatccg gccgcggtag tcgtacagcc
     3121 aaggcaccca acgcagtaac agccctcgtg tgggtcggat gtgggtagcg tgtccgctga
     3181 ccgtgcggcc cgcggtcgcg gcgattaaaa tgcctgttcg aatgacggag cggctttgaa
     3241 cgtagcactg tttatcacat gcctgaccga ctcgttcttc ccgcgcgccg gggaggcggt
     3301 ggtgcgcgtc ctccggcatt tcggctgccg cgtgcaattt cccgccgccc agacctgctg
     3361 cgggcagccg gcttacaaca gcggttttca cgatgaggcc cgcgtgctgg cgcggcgcat
     3421 gatcgaaatc tttgaaccct acgaaaccat cgtcagcccg tcggcctcct gcgtcgcaat
     3481 ggtgaagcag cacttcgccc ccctgctggc ggacgagccg gcctggcgcg cccgggcgga
     3541 gcggctggcc gaacgcacgc acgagttcgc caccttcctc gtcgaccggt taaacgtgga
     3601 tatcgccggt ctgctgcgct tcgacgagcc ggtcaccttc cactatccct gtcacgcgcg
     3661 cggcatatac ggcgcggagg atttgcagcg ctggctgacg cgcgacgggc aggtgaatct
     3721 gcgcctcccg cagcacgtgg actgctgctg cggcttcggc ggcatgttcg ccgtcgatta
     3781 ccccgagatc agcacggcca tgctgaacga caagctcgac ttgctggcgg ccaccggagc
     3841 ccgactcgta atctgcaacg aaggcggctg cacgctgcag atggccggcg gagcgcggcg
     3901 gcgcggcctg ccgctgcg
//
LOCUS       DAOSYS010000307         3915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18704_length_3915_cov_4.323316, whole genome shotgun sequence.
ACCESSION   DAOSYS010000307 DAOSYS010000000
VERSION     DAOSYS010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18704_length_3915_cov_4.323316"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1032
                     /locus_tag="PLU99_14330"
     CDS             223..1032
                     /locus_tag="PLU99_14330"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO18268.1"
                     /translation="MSGQRLDFYRHGLAEFIPDRDANILVCAAGPTDRNVFRELGYRR
                     VTMVNLDTRLDAAKLAPYQWQRQDIQALDFADASFDYAVTHASLHHCRSPHRALLEMY
                     RVARVGVLVFESRDSFTMRLMQRLGITLSYEDAAVFYNDCRYGGVDNSPIPNFVYRWT
                     EREVEKTICSYDPTARHRIHYRYWYLFPQLARLERRASLKHLAVRLAVLPYLAYVTLF
                     PRQRNRFCFFIEKPKLPEALHPWLKMRDDGQIEFNRTWGDKRYKATLPPED"
     gene            complement(1091..1582)
                     /locus_tag="PLU99_14335"
     CDS             complement(1091..1582)
                     /locus_tag="PLU99_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOO18269.1"
                     /translation="MDPASRTVSSDAPATAPRSSAGARWVAAAVFVGCAAVLAMAVWL
                     EPDPRGFGTHEQFGSAPCGMLVLTGYPCPTCGMTTAFAYFVRAQWIRSFMTQPAGFVL
                     ALATAGFALLSLRTLILGRWPQRFLPTISSYWIFLALLILLLGGWGFKLVEGLVTGTL
                     PLH"
     gene            complement(1753..2220)
                     /locus_tag="PLU99_14340"
     CDS             complement(1753..2220)
                     /locus_tag="PLU99_14340"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOO18270.1"
                     /translation="MQSNGIKNETQFSVFLVNKPGILARVCQRLADDRVNILALSMMD
                     STEHGVLRMVVEDPARARQSLAELDVPTTESQVLVATLPNRPGSLADVVERLSVAHVN
                     VNYAYCTTGAPGGKSLGVFRVSDLKKAISVLEERRPRRKLPSTAVRSPGQRRR"
     gene            complement(2547..2729)
                     /locus_tag="PLU99_14345"
     CDS             complement(2547..2729)
                     /locus_tag="PLU99_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18271.1"
                     /translation="MTSRLQRALMLVVLLVGVGSLTACDEDDWEDYFEDWGGCSCGCR
                     YDDRCGGGHWYEFWYW"
     gene            complement(2906..3421)
                     /locus_tag="PLU99_14350"
     CDS             complement(2906..3421)
                     /locus_tag="PLU99_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18272.1"
                     /translation="MAHRGTARNVAGLLGLIVLMLLVGTCMMYVREQRRFCPLPDNPA
                     RGWHLSGTIERYAAGDMYLKIGRAADSFQQFDVQQLRFATYAREGTGGVTVEVYCYDM
                     ASRAHALAAYQSEKSAGASAVPAGDGGHRSGASVSFLRGSSYVQVISIHYGEADEGDI
                     RTLAAALGGTR"
     gene            3515..>3915
                     /locus_tag="PLU99_14355"
     CDS             3515..>3915
                     /locus_tag="PLU99_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18273.1"
                     /translation="MPQAPPHQQTTATPPPHAAGRSLRLWLAALVLAWGAQAIVTQSL
                     LLREAMVLMYGSEFAWGVVLFAWLVGIALGAAVGGRLAGALGRRGSVPGGLAGVLLAL
                     SLAGVLELWVFRGARAWMGVAPGELLPLGRTV"
BASE COUNT          692 a         1334 c         1210 g          679 t
ORIGIN      
        1 agggctgggt ccgccggtgc ggccccgata ctttgggcag cccgccaaac tgggctcgcc
       61 cagcgcccgc tttcgcaccg cggactgcgc ccgactgcac gcttacagcc cttgcgccgg
      121 atttgaaacc ctcgcccgcc gggcttaatc tccggcgcct ccgccggcag atcaaggttg
      181 acgcgcgagc cggcaaacgg cgccggacac tggtggccaa cgatgagcgg acaacggctg
      241 gacttctatc gtcacgggtt ggccgagttc attcccgacc gcgatgcgaa cattctcgtc
      301 tgcgccgccg gcccgactga ccgtaacgtc ttccgtgagc tcggctatcg ccgcgtgacg
      361 atggtcaact tggacacgcg cctcgacgcc gccaagctgg ccccctacca atggcagcgt
      421 caggacatac aggcactcga ttttgccgat gcctccttcg actacgccgt aacgcacgcc
      481 tccctgcacc attgccgctc gccgcatcgc gccttgctgg agatgtaccg cgtggcgcgc
      541 gtgggagtgc tcgtattcga atcacgcgac agcttcacga tgcgcctcat gcagcgtttg
      601 ggcattaccc tctcgtatga ggacgccgcc gtgttctaca acgactgtcg ttacggcggc
      661 gtcgacaact cgcccatccc caacttcgtc taccgctgga ccgagcgtga agtcgaaaag
      721 acgatatgct cgtacgaccc cacggcgcgc caccgtattc attaccgcta ttggtacctc
      781 ttcccgcagt tggcacgcct cgaacgtcgc gcctcgctaa aacatctagc ggtacgactg
      841 gcggtcctcc cgtacctggc ctacgtcacg ctctttcctc gccagcgcaa tcgcttctgc
      901 tttttcatcg aaaagccgaa gctgcctgaa gcgctgcacc cctggctcaa gatgcgcgac
      961 gacggccaaa tcgagttcaa ccggacctgg ggcgacaagc gctacaaggc aactctgccg
     1021 cccgaagatt aggacagcct ggctgcaagc tcttgcgccc tgatcctttg ggctccagcc
     1081 gaccaagctt ttagtgcagc ggcagcgtgc ccgttaccaa gccctcaacc agcttgaagc
     1141 cccagcctcc caatagcagg atcagcagcg ccagaaaaat ccaatagctg gaaatcgtcg
     1201 gcagaaaacg ctgcggccag cggcccagga tcagcgtccg cagcgagagc agcgcgaagc
     1261 cggccgtcgc cagtgccagc acaaagccgg ccggttgggt catgaaggag cgaatccact
     1321 gggcccgcac gaagtaggcg aaggcggtcg tcatgccgca ggtcgggcag gggtagccgg
     1381 tcagtacgag cataccgcag ggcgcgctgc cgaattgctc gtgcgtgccg aagccgcgcg
     1441 ggtccggctc gagccaaacc gccatagcca gcacggccgc gcaacctacg aaaacggcgg
     1501 ccgctaccca gcgggcgccg gccgacgaac gcggcgccgt ggccggcgcg tccgagctaa
     1561 cagtccggga tgcagggtcc aacgctatct ccgacgctgc tttttctgac gctcgctacg
     1621 tccgacggcg actttctctg acgccaacta tttcagactc gctatctccg ataccgaggc
     1681 tatcaccgat gcgggccacc tgccaccgac cccgactaat ctccgacact gaggatatct
     1741 tcgccgctac ggctacctcc gccgctgacc cggactgcga acggccgtcg aggggagctt
     1801 tctgcggggg cggcgctctt cgagcacgct aatggccttt ttcaggtccg acacgcgaaa
     1861 aacgcccagc gacttgccgc cgggtgcgcc ggtggtgcag tacgcgtagt ttacgttgac
     1921 gtgggccacc gagaggcgct cgaccacgtc ggccaacgaa ccggggcgat tggggagggt
     1981 ggccaccaga acctgcgatt cggtcgtggg cacgtcaagc tcggccaggc tctggcgggc
     2041 gcgggccggg tcttctacga ccatgcgcag cacgccgtgt tcggtcgaat ccatcatgct
     2101 gagggcgaga atgttgacgc gatcgtcggc caggcgctgg cagaccctgg ccagaatgcc
     2161 cggtttgtta accaggaaca cggagaactg cgtttcgttt tttatgccgt tggactgcat
     2221 cggtcttagc tcccgtgtgc ggtttcaagg tgtaaacggc cgccgggccg ggcgggtcag
     2281 catatgcttc attgtatggc ccgttttgtt ggctttcgac caacgccggg tcggagggcg
     2341 tcgtggcacg gttatcatga taccgccgca aatggacgaa gcccagccgc ggcaatacgc
     2401 cggcgctgat ggatggacgc cagcggtgat ggataggcgg taggcggacg tggcgatggc
     2461 gcgggggccg gcggagcggg cactacgcgg atgcggaggc tcttggcggc atttcggggc
     2521 ggcacgcccc ggctctgctt atccgatcac caataccaga attcgtacca gtggccgccg
     2581 ccacagcggt catcgtaacg gcagccgcaa ctacagccgc cccaatcctc aaaataatcc
     2641 tcccagtcgt cctcgtcgca ggcggtcaag ctacccacgc ccaccagcaa aacgaccaac
     2701 atcaacgccc tttgtagcct gcttgtcacg ctcattctcc tcgccgccgg caccccggtc
     2761 gctgcgcttg taggccacgc ggggccggcc aaaacgccaa aacccaaccg tgcacatcag
     2821 tattgacacg tgccgcgccg ccaggtgagc gggctgcccg aaacgtgcag cgcgtcaggc
     2881 gggcggcgcg cccgcgccgc tgcgcctacc tcgtcccgcc caatgccgct gccaacgtgc
     2941 gaatatcccc ttcgtccgcc tcaccgtagt gaattgaaat aacctgaacg tagctgctgc
     3001 cacgcagaaa agatacgctc gccccgctgc ggtgaccgcc gtcgccggct gggacggcgc
     3061 tcgcgccggc cgatttctcg gattggtagg ccgccagcgc gtgcgcgcgc gaggccatgt
     3121 cgtaacagta gacctccacc gtaacgccgc ccgtcccctc ccgcgcatac gttgcgaacc
     3181 ttaactgctg gacgtcgaac tgctggaagc tgtccgcggc gcggccgatc ttcagataca
     3241 tatccccggc ggcatagcgt tcaatcgtac cggacaaatg ccagccccgc gccggattgt
     3301 ctggcagcgg gcagaagcgc cgctgttccc ggacgtacat catgcacgtg cccaccagta
     3361 gcatcagcac gatgagcccc agcagccccg ccacgttgcg ggctgtgccg cgatgtgcca
     3421 atagattcac ctgcctgccc gaaaagcacg accctggctc aactccctgg ccggagccct
     3481 tatgatacca caagcgatcc gccggagaat gcacatgccc caggcgccgc cgcaccagca
     3541 aaccaccgcg acgccgccgc cccatgccgc cggccgcagt ttacgtcttt ggctggcggc
     3601 cctggttctg gcctggggcg cgcaggccat cgtcacgcag tccttgctgc tgcgcgaagc
     3661 catggtgctc atgtacggca gcgagttcgc ctggggcgtg gtgttgttcg cctggctggt
     3721 ggggatcgcg ctcggggcgg cagtcggcgg tcgattggct ggcgcgctgg ggcgacgcgg
     3781 gagcgttccc ggcggcctcg cgggcgtgtt gctggcactg agcctggccg gagtgcttga
     3841 attgtgggta ttccgcgggg cgcgggcctg gatgggtgta gcgccgggcg agctattgcc
     3901 gctggggcgg acggt
//
LOCUS       DAOSYS010000308         3884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_18898_length_3884_cov_5.661008, whole genome shotgun sequence.
ACCESSION   DAOSYS010000308 DAOSYS010000000
VERSION     DAOSYS010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18898_length_3884_cov_5.661008"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLU99_14360"
     CDS             complement(<1..182)
                     /locus_tag="PLU99_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOO18274.1"
                     /translation="MIQNWHAKRVTVMGLGRFGGGVGVTRWLARAGARLTLTDKLPAA
                     ELGDSLAQLEGLEINYR"
     gene            335..817
                     /locus_tag="PLU99_14365"
     CDS             335..817
                     /locus_tag="PLU99_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOO18275.1"
                     /translation="MDEIRIRIATLADLETIAANNIAMAAETENLELDPATARSGVRR
                     VLTDTQRGTYFLAERAGKVVGQLLITREWSDWRDDWFWWLQSVYVVPEARGVGIYTAL
                     HHHVEQLARQSPGVCGLRLYVDRRNATAIQVYQQLGMQQTNYDLMELAWKKPRRGTNA
                     "
     gene            1070..3121
                     /gene="argS"
                     /locus_tag="PLU99_14370"
     CDS             1070..3121
                     /gene="argS"
                     /locus_tag="PLU99_14370"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOO18276.1"
                     /translation="MTNESLIELLRRHFRDAIAEITGKRAEDVDAMIRPAGDPKFGDY
                     QCNVAMSLAKELRAKPREVAERIVAAVQPRLGGLVEPLEIAGPGFINIRLKPAALGDY
                     LGEIPRPPEETLEATPSHVPASPASETFADRLGIAPVAQPQRIVVEYSQPNIAKQMHV
                     GHLRSTIIGDVFARVLAFEGHDVIRQNHIGDWGTQFGMLIRWYREHPLPTPQTHADVL
                     EAIESDYRAAQKRFEEDPKFADEARRAVAELQGGDPATRELWRQMCAVSQEAMNELYT
                     RLGVLLRPEDVRGESFYNDQLPDVIRELREKLPPRVASAPREVPPVASAPRGRRVELG
                     EGSGDVKPAPWAEVRDDQGAVCVFLWDEKGEPPFKSPEGQPLPLIIQKSDGAYLYATT
                     DLAAVRYRLRDLQFSSGVGAERVIYVTDARQKLHFEMFLAVARAAGWVQPRHRIDHVT
                     FGSVLGEDRRPLKTRAGGTVKLRELLDEAENRARALIEERARTKQAEPDDAQPDEQQA
                     SPETPADSAEMAEIARRVGIAAVKYADLRNDRRTDYIFSWDKMLALTGNTAPYMMYAY
                     ARIRSIYRKAAERIGSPDVYAPGVRLTLIEPAELALGLRLARLRETIDVIAADLEPHV
                     LCTYLYELAGDFMRFYETCPVLRAPDDATRLSRLRLCDLTARTLKLGLWLLGIETIER
                     M"
     gene            complement(3262..>3884)
                     /locus_tag="PLU99_14375"
     CDS             complement(3262..>3884)
                     /locus_tag="PLU99_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18277.1"
                     /translation="AMVTRGPSQTVMRCTKGMTYALGSEGPERSDSAVFDGNVRFIHV
                     AGREMVRLEEMLPEVHSDPGLLAQLKSRNTSLDCDRLECGFLVAGAEASRAGAMSSRD
                     TRLKWLLASGNAELRDEREVLAKQPGSKTAQRWESVVRTVFAHQLDFDLEQRVVRVLG
                     SAAAKARIYEENPDSAEARILVGEEFSIDLQNRTVRSGPSHGTIGR"
BASE COUNT          730 a         1269 c         1248 g          637 t
ORIGIN      
        1 cggtaattaa tctcgaggcc ctcgagctgc gccagtgagt ctccaagctc cgccgccgga
       61 agcttgtcgg tcagggttag ccgtgccccg gcgcgggcca gccagcgcgt gaccccgacg
      121 ccgcccccga agcggcccag ccccatcacc gtgacgcgtt ttgcatgcca attctggatc
      181 atcacgctta tagccgctag gctagttggt atcgaaccga atccggtgtc gtgccattaa
      241 ccggcgccaa cgcggagccg ggcgggaccg gattcgcgac gttgggcccg gcccgtggat
      301 aagttcgcag cgccagactg aggcgtcaac cgcaatggac gaaatccgta tccgaatcgc
      361 tacgcttgcc gacctggaaa ccatcgccgc caacaacatc gccatggcgg ccgagacgga
      421 gaacctggag cttgacccgg ccacggcgcg atcaggcgta cgccgggtac tgacggacac
      481 gcagcgtgga acgtacttcc tggccgagcg tgcgggcaag gtggtcggcc aattgctgat
      541 cacgcgcgag tggagtgact ggcgcgacga ttggttctgg tggctgcaaa gcgtctacgt
      601 ggtgcccgaa gcccgcgggg tgggcatata caccgcgctg caccatcacg tcgagcagct
      661 cgcccgccaa tcgcccggtg tctgcggcct gcggctgtac gtcgaccggc gtaatgccac
      721 ggcgattcag gtataccagc aactcgggat gcagcagacc aattacgact taatggagct
      781 ggcgtggaag aaaccgcggc ggggtacgaa cgcatgaccc cgacgtcagc ctgccagggc
      841 gaggtgtccg ccggctgctg tgccgagcgc ctgcgcgggc acgccaaacg caccaagccg
      901 tgtgcttgtg caccgattcc gcgcccagct acattgccgg cacgggcgaa ccggaatcca
      961 caccaccgct ctcccaagcc gaaacacacc catcgaatag ggacgcagag tacgtgcaag
     1021 ggacgcagag tagttgaaac gtgccggcgc gcccggtagc ctctctccaa tgaccaacga
     1081 aagcctgatc gagctgttgc ggcgccattt tcgtgacgcg atcgccgaaa taaccggcaa
     1141 acgcgccgaa gacgtcgatg ccatgatccg ccccgccggc gatccgaaat tcggcgacta
     1201 ccagtgcaac gtcgccatgt cgctggccaa ggagctgcgc gccaagccgc gcgaagtggc
     1261 cgagcgtatc gtggccgcgg tgcagccgcg cctgggcggt ttggtcgagc cgctggagat
     1321 cgccgggccg ggcttcatca acatccgcct gaagccggca gcgctaggcg actacctggg
     1381 cgagattcct cgtccgccgg aagagacctt ggaggcgacg ccgtcacacg ttcccgcctc
     1441 gccggcttcc gaaacgttcg ccgatcgcct ggggatcgcc ccggtcgcgc agccgcagcg
     1501 catcgtggtc gagtattcgc aacccaacat cgccaagcag atgcacgtcg ggcacctgcg
     1561 cagcacgatc atcggcgacg tttttgcgcg cgtgctggcg ttcgaaggac acgacgtcat
     1621 tcggcagaat cacattggcg attggggtac gcagttcggc atgctcatcc gctggtatcg
     1681 cgagcacccg ctgccgacgc cgcagacgca tgctgacgtg ctggaagcga ttgaaagcga
     1741 ctaccgcgcg gcacaaaaac gttttgagga agaccccaag ttcgcggacg aggcgcgccg
     1801 cgctgtggcg gaactgcaag gcggcgatcc ggccacacgg gagctgtggc ggcagatgtg
     1861 cgccgtctca caggaggcca tgaacgaact ctatacacgc ctcggtgtct tgttgcggcc
     1921 ggaggacgtg cgcggagaga gcttctataa cgaccaattg ccagacgtta tcagagaact
     1981 ccgggaaaag ctgccgccgc gcgtagcgtc ggccccccgc gaagtgccgc ccgtagcgtc
     2041 ggccccccgt ggccgacgtg ttgagttggg agaaggcagc ggcgacgtga aacctgcgcc
     2101 gtgggcggaa gtccgcgacg atcagggggc ggtatgcgtt ttcctttggg atgagaaagg
     2161 cgagccgccc ttcaagtcgc ctgaagggca gccgctgcca ctgataattc agaagtctga
     2221 cggcgcttac ctgtatgcga cgaccgattt ggcggcggtt cgctaccggc tgcgcgattt
     2281 gcagttttcg tccggagttg gggcggaacg cgtcatctac gtgactgacg cccggcagaa
     2341 gctgcatttt gagatgttcc tggcggtggc ccgggcggca ggctgggtgc agcccaggca
     2401 tcgcatcgat cacgtgacct tcggcagcgt gctgggcgag gatcgccggc cgctgaagac
     2461 gcgcgccggg ggaacggtga agctgcgcga attgctggac gaggcggaaa accgggcgcg
     2521 ggcactgatc gaagaacggg cccggacgaa gcaggctgag ccggacgacg cacagcctga
     2581 tgagcagcag gcgtccccgg aaacgccggc cgacagcgcc gaaatggcgg agatcgcgcg
     2641 ccgcgtgggc atcgcggccg tcaaatacgc cgacttgcgc aacgaccgcc gcacggacta
     2701 catcttcagt tgggacaaga tgctggcgct gaccggcaac acggcgcctt acatgatgta
     2761 cgcctacgcg cggattcgat ctatttaccg caaagccgcg gagcgcatcg gttcgccgga
     2821 tgtctacgcg cccggcgtcc gcctgacctt aattgagccg gcggagttgg cgctgggact
     2881 gcgcctggcg cggctgcgcg agactattga cgtgatcgcg gcggatctgg agccacacgt
     2941 gctctgtacg tacctgtacg aactggcggg cgacttcatg cggttctatg agacgtgtcc
     3001 ggtgctccgc gctcccgacg acgccacgcg cctcagccgt ctgcgtctgt gcgatctgac
     3061 cgcccgcacg ctgaagctcg gcctctggct gctcggaatc gagacgatcg agagaatgta
     3121 gcagagccgg ctcgtgtcga gccggaaggg tcgttgtgcg cacaatcgcg caacggggca
     3181 aaaccgtgct gaggggtcgc cttgaacaca ttgcggggcg acacgcccca gctctgatct
     3241 cagatccgcc cccgctctgc tctatcttcc aatcgtgccg tgcgaaggac cgctgcgcac
     3301 ggtgcgattc tgcagatcga tgctgaattc ctcgcccacc agaatgcgcg cctcggcgga
     3361 atccgggttc tcctcgtaaa tgcgcgcttt agcggccgcc gacccaagca cgcgcaccac
     3421 tctttgctcc agatcaaagt caagctggtg ggcaaagaca gtgcgaacca ccgattccca
     3481 gcgctgggcg gtcttcgaac cgggctgctt ggccagcact tcgcgctcgt cgcgcaactc
     3541 cgcgttgccc gacgccagca gccacttcag ccgcgtgtcg cgcgatgaca tcgccccggc
     3601 gcggctcgcc tccgcccccg caaccaagaa cccgcactcc agccggtcgc aatccaacga
     3661 agtgttgcgg ctcttgagct gggccagcaa accggggtcg ctgtgaacct ccggaagcat
     3721 ttcctccagc cgaaccatct cgcgcccggc gacgtgtatg aaacgtacgt tgccgtcaaa
     3781 tacggccgaa tccgagcgct ccggcccctc cgaccctaac gcgtacgtca tccccttagt
     3841 gcagcgcatc accgtctgac tcggaccgcg cgtcaccatg gccg
//
LOCUS       DAOSYS010000309         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19059_length_3856_cov_4.947119, whole genome shotgun sequence.
ACCESSION   DAOSYS010000309 DAOSYS010000000
VERSION     DAOSYS010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19059_length_3856_cov_4.947119"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PLU99_14380"
     CDS             complement(<1..1027)
                     /locus_tag="PLU99_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO18278.1"
                     /translation="MAITYLDNNATTRVDPRVLAQMLPYFSEEYGNPSSAHQFGSRVA
                     GRIEEARTQVARLIGARETEIIFTSGGTEADNLALRGVLAARASKRHVVISAVEHHAI
                     FEPCEALQREGFQVTSIAVDREGRLDLDQLAANIRDDTAIVSIMLANNETGVIFPLRE
                     VAAIAHQRGVPVHTDAVNALGKTRVNVEELGVDLLSLSSHKIYGPKGVGALYVRRNTP
                     LRAMMIGGGQERKRRGGTLNAPGIIGLGAACQILQELGDGGMEQVGALRDQLEAELRR
                     RFQDILILGAAAERLPNTCCACFPGLSSEPLVMLLSENGICVSSGAACSSGSLEPSRV
                     VQAMGIEP"
     gene            complement(1147..2226)
                     /locus_tag="PLU99_14385"
     CDS             complement(1147..2226)
                     /locus_tag="PLU99_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOO18279.1"
                     /translation="MRPAGPIKALVIQSRPTLWRRLTQLLARHPEVRIGNWAADAWEA
                     RLAVIEHHPDVILLDVGLSDWATLLRSLLQYYPVPVIAIGQSEPQSRTRILQAMARGA
                     MDFITEPEAEDALAWGKLADELAQKLHVAARDFRPVSPARLAAWRARSFAEGGLDPRR
                     FVVGVGASTGGPEALRIMLANAPPDFPPVLIVQHMPAMFTASFAERLDRFSAMSVAEA
                     RQGDLLSPGRALVARGDTHLTARRTGQSCRVCYSHQRPIGRHCPSVDVLFESLLPFGE
                     RAVGVLLTGMGDDGARGLLRLRQAGALTLAQNRESCAVYGMPKVAAEIGAAVLTAAPA
                     DIPELIRRTLAARTGAKAAAGGARK"
     gene            complement(2351..3193)
                     /locus_tag="PLU99_14390"
     CDS             complement(2351..3193)
                     /locus_tag="PLU99_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOO18280.1"
                     /translation="MNVIETLRDLTAEEYELFRQLVYQHSGINLGGQKMQLVRARLGK
                     RLRQGGFRSFRAYYEYVRKDASGRELCAVIDAIATNTTHLFREKQHFDFLGQVLRDWA
                     SDEVWRRRRHDVRIWSAACSSGEEPYSIAMTAHDALCTYTGIEVKILATDISTNVLER
                     AEQGTYEQHRLGTVPESFRRRYFTAAGQGLMRVVPELRRMITFARLNLIAGNFPFRYP
                     FDLIFCRNVMIYFDRPTQATLVNRLAEHLRPGGYLLIGHSESLNSISHPLTYVRPTIY
                     QRRR"
     gene            complement(3215..3706)
                     /locus_tag="PLU99_14395"
     CDS             complement(3215..3706)
                     /locus_tag="PLU99_14395"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOO18281.1"
                     /translation="MDFEHRMKQCPLQTPAKPDVGVLVNEVLGRLAYLIGEDEAPPGA
                     VFTDWLHFEINHCGPTAGTLRCWCTRKFAHQLAANLLGVEPQPDEAAAAAEDACREFI
                     NVLCGQLVTAWYGTGAVFTLTIPTVCGEAHVRPPENLGEQDCRVYVAGEPLYCQYVAD
                     PPG"
BASE COUNT          644 a         1296 c         1228 g          688 t
ORIGIN      
        1 gcggctcgat gcccatggcc tgtaccacgc gcgacggctc cagggaaccg ctgctgcagg
       61 ccgcgccgct ggatacacag atgccgttct cgctcagcag cattaccagc ggctcagaac
      121 tcaggccggg gaaacaggcg cagcaggtgt ttggcaggcg ctcggccgct gccccgagta
      181 ttaagatgtc ctggaagcgc cgccgtagct ccgcttcaag ttggtcgcgc agcgcaccta
      241 cctgctccat gccgccgtcg cccagctcct gcagaatctg acacgccgcg cccagaccga
      301 tgatgcccgg cgcgttcaaa gtccccccgc gccgcttgcg ctcctgtccg ccgccgatca
      361 tcattgcccg cagcggcgtg ttgcgccgca cgtagagcgc gccgacgccc ttcgggccgt
      421 aaatcttgtg gctgctgagc gaaagcagat cgacgcccag ttcttcgacg ttgacgcgcg
      481 ttttgccgag ggcgttgacc gcgtccgtgt gtaccgggac gccgcgctgg tgggcgatgg
      541 cggcgacctc gcgcaatggg aaaatcacgc ccgtttcgtt gtttgcgagc atgatggaga
      601 cgatggcggt atcgtcgcgg atgttggccg cgagctggtc gaggtcgagc cgtccctcac
      661 gatcgacagc gatcgacgtg acctggaatc cctcgcgctg gagcgcctcg catggctcga
      721 atatggcgtg atgctcgacg gccgagatga cgacgtgccg cttgctggcc cgggctgcca
      781 gcacgccgcg cagcgcgagg ttgtcggcct ctgtgccgcc gctggtgaag ataatctccg
      841 tctcgcgcgc tccgatcagc cgtgccacct gagtgcgggc ctcctcgatg cgcccggcga
      901 cgcgcgagcc gaattggtga gcgctggagg ggttgccgta ttcctcggag aagtacggca
      961 gcatttgcgc gagcacgcgc gggtcaacgc gcgtcgtcgc gttgttgtcg aggtaagtga
     1021 ttgccacgcc ggctccgatc cgctgagaac tcttacagca ttttagcccg cagcattcta
     1081 gtcgagcagc gttcgtcggc cagtgtgccc gcggcggccg gccgttgggc tacgcggaga
     1141 ttgacgttac ttccgcgcgc cgcccgcggc agctttcgcg cccgtgcgcg ccgccagcgt
     1201 ccgccggatc agttcgggta tatctgccgg agcggccgtt aatacggccg cgcctatttc
     1261 ggcggcaacc ttgggcatac cgtacacggc acatgattca cggttttgcg ccagtgtcag
     1321 cgcgccggcc tgccgcagcc gcagcagtcc gcgggcgccg tcgtcgccca tgccggtgag
     1381 cagcacgccg acggcgcgct cgccgaatgg caaaagtgat tcgaatagca cgtccacgct
     1441 cggacagtgg cggccgatcg gtcgttggtg actgtagcag acgcgacatg attggccggt
     1501 ccgccgcgcc gtgaggtgcg tgtcgccgcg ggccaccagg gcgcggccgg gtgacaaaag
     1561 gtcgccctga cgggcctcgg cgacgctcat ggcgctaaag cgatcaagcc gctcggcgaa
     1621 gctggcggtg aacatcgccg gcatgtgctg cacgatcaat accggcggaa agtcgggcgg
     1681 agcgttggcc agcatgatgc gcaacgcctc cgggccgccg gtcgaagcgc caacgccgac
     1741 cacgaagcgc cgcgggtcga ggccgccttc cgcaaatgac ctggcgcgcc aggccgccag
     1801 acgcgccggc gaaacggggc gaaaatcgcg ggccgcaaca tgtagtttct gggccagttc
     1861 gtccgcgagc ttcccccacg cgagcgcgtc ctccgcctcg ggctcggtga tgaaatccat
     1921 cgcgccgcgc gccatggcct gtaggatgcg cgttctgctc tgaggttcac tttgcccaat
     1981 ggcaatcacg ggcaccggat aatactgaag cagactgcgc agcagcgtcg cccaatccga
     2041 aagccccacg tcgagcagaa tcacgtccgg atgatgctca atgaccgcca gccgggcttc
     2101 ccaggcgtct gccgcccagt tgccgattcg cacttccggg tgccgcgcca ggagttgcgt
     2161 cagacgacgc cacagcgtcg gccgcgactg gatcaccagg gccttgatcg gccccgccgg
     2221 cctcatgccg cggcctccgc gcggcgcgcc gcgtcgttgc gatcatcgac gcaggcatgc
     2281 ccgcgcctcg cgcctgggag ggacgcccgt gcttctggtg gcctcgttgg cggcttgcct
     2341 tccacggacc tcatcggcgc ctctgataaa tggtcggccg aacgtaggtc agcgggtgac
     2401 tgatcgaatt caggctttca gaatgcccga tcagcaaata tccgcccggc cgcaggtgtt
     2461 ccgccaggcg attcaccaac gtggcctgcg tggggcggtc gaagtagatc attacattgc
     2521 ggcagaaaat gaggtcgaac ggatagcgaa acgggaaatt gcccgcgatc agattcagcc
     2581 gcgcgaacgt gatcatccgc cgcagctccg gcacgacccg catcaggccc tggcccgcgg
     2641 ctgtgaagta gcgccgccga aacgactcgg gcactgtccc caggcggtgt tgctcgtacg
     2701 tgccttgctc ggcccgctcc agcacgttgg tcgaaatatc ggtggccagt atcttcacct
     2761 caatgcccgt gtacgtgcac agagcatcgt gcgccgtcat cgcaatggag tagggctcct
     2821 cgccgctgga acacgccgcg ctccagatgc gcacgtcgtg gcggcggcgg cgccagacct
     2881 cgtcgctcgc ccagtcgcgc aacacctgtc cgagaaagtc gaagtgctgc ttctcgcgaa
     2941 acaggtgcgt ggtattcgtg gctatggcgt cgatcacggc gcacagttcg cggccgctgg
     3001 cgtccttgcg cacgtattcg taataggcgc ggaaggatcg gaagcccccc tggcgcagcc
     3061 gctttcccag ccgtgcccgc accagttgca tcttctgccc gcccagattg atgccactgt
     3121 gctggtacac cagttgccga aagagctcgt actcctcggc cgtcaggtcc cgcagtgtct
     3181 caatgacgtt cattcacgtg ctcaccccag ggcctcaacc gggcgggtcg gccacatact
     3241 ggcagtacaa cggttcgccc gccacatata ctcggcagtc ctgctccccc agattttccg
     3301 gcgggcgaac atgtgcctcg ccgcaaacgg tcggaatggt caaggtgaac accgcccccg
     3361 tgccgtacca ggccgtcacc aattggccgc atagcacgtt gatgaactcg cggcaggcat
     3421 cctcggccgc cgccgcggcc tcgtccggct gcggttcgac ccccagcagg ttggccgcca
     3481 gttgatgcgc gaatttccga gtacaccagc agcgcaacgt gccggcagtg ggaccgcagt
     3541 ggttgatttc aaagtgcagc caatcggtaa agaccgcacc cgggggcgcc tcgtcttcgc
     3601 cgatcaggta tgccagccgg ccgagcacct cattgaccag gacgcccacg tcgggtttgg
     3661 cgggcgtctg tagtgggcat tgtttcatcc ggtgttcgaa gtccataagc acttcctgtg
     3721 aagcttcttc aacctggtcg ccacggccgc cggctacggc gccggtgtac ttacgtcgat
     3781 ttcgcaactc ggcagaatcg ctactgagcc cgtacgcgaa ttgaccgcca caatttccgt
     3841 agcgttctca atcttg
//
LOCUS       DAOSYS010000310         3837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19164_length_3837_cov_5.542834, whole genome shotgun sequence.
ACCESSION   DAOSYS010000310 DAOSYS010000000
VERSION     DAOSYS010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_3837_cov_5.542834"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1222)
                     /locus_tag="PLU99_14400"
     CDS             complement(20..1222)
                     /locus_tag="PLU99_14400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOO18282.1"
                     /translation="MPEKTYTAAPDLLADLDSASLPGELRTEEMLINMGPQHPSTHGV
                     LRLVLRTDGEMVLEVTPHMGYLHRCAEKVGENVAPFQFIPITDRLDYLAAMNNNQAFS
                     IAVEKLAGLEVPERGRYIRIIMAELNRIASHLVSFGTFGLEMGAFTPLLFGFREREYI
                     LDMFEATCGARLTYSYITIGGPNQDLPEGFLEKLAWYLDYLEPKIDEYNNLLTYNHIF
                     VKRTAGVGVISAEDAIAWGLTGPCLRGSGVKWDLRKCEPYDIYDRFDFDIPVGVDGGS
                     NGIPKGVTVGDSWNRYFVRISEIKQSIRILRQCLEQIPGGDFRTKVPRVLKLPPKEVY
                     FEAEGARGQVGFLIVGDGTPIPRRVKIRGPSFCNLSILPHVCRNVLLADIVAIAGSID
                     VVMGEVDR"
     assembly_gap    1368..1467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1468..1721)
                     /locus_tag="PLU99_14405"
     CDS             complement(<1468..1721)
                     /locus_tag="PLU99_14405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOO18283.1"
                     /translation="MTPQEIAEILKQRFGDAITEVAADALHPQVTVAAERWHEVALFL
                     RDEPHLAMNMLRCISGIDRHPEPYIDLVYDVVSMQLGKANG"
     gene            complement(1778..2275)
                     /gene="nuoB"
                     /locus_tag="PLU99_14410"
     CDS             complement(1778..2275)
                     /gene="nuoB"
                     /locus_tag="PLU99_14410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOO18284.1"
                     /translation="MGWLQNRFEENFAITTVDWALNWARANSIWPMTFGLACCAIEMM
                     ATASSRWDLDRFGAGAYRATPRQSDLMIVAGTVTFKMASRIRRLYNQMPDPKYVLAMG
                     ACAIGGGPYFKYGYHVVKGVDLIVPVDVYVPGCPPRPEALVEGLLRLQDKMRQQTIAK
                     QAEAV"
     gene            complement(2281..2853)
                     /locus_tag="PLU99_14415"
     CDS             complement(2281..2853)
                     /locus_tag="PLU99_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOO18285.1"
                     /translation="MVFAHASLLAEGGTDLLQILAIFAVAAFFFGFGALLVGRLLRPN
                     RPNPDKITAYECGELPLGPGWVQFDLRFYVVALVFLVFDVELALFYPWAVVFGGGGTT
                     EAGGAATADALQSIRWAAMWDMLFFFGVIVVGFLFLWRFGYLDWVRTTRTARAEARPP
                     TTENGTRTLDDARLRQTVNEFAEAPAQART"
     gene            complement(2968..3612)
                     /locus_tag="PLU99_14420"
     CDS             complement(2968..3612)
                     /locus_tag="PLU99_14420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOO18286.1"
                     /translation="MRKHIRDSLEAVRTILIGMGITLRYCFARTVTVQYPDVPPTVKP
                     RFRGFHWYEIERCSACGNCARACPVDCIYIEKSGPRKIEKETGISRGGAMTRYAIDYS
                     KCMFCGLCTENCPTDCIHMGDLHDISGFTRQDTVVEFTELARQGLQSPLPLWMKKKRL
                     PAWAQALKEAWQRRAEPRREEMLKALTEQPVAKPPPAAKPAAEPPAKPAPPPAE"
BASE COUNT          686 a         1201 c         1133 g          717 t
ORIGIN      
        1 ggcagcttga cgcagttcgt catcgatcca cctcccccat aaccacatca atactcccgg
       61 cgatcgccac gatatctgcc agcaaaacat tgcggcagac atggggaaga atggagaggt
      121 tgcagaacga ggggccgcgg atcttcaccc gtcgcgggat gggcgttccg tcgcccacaa
      181 tcaggaaacc gacctgcccg cgcgcccctt ccgcctcgaa gtacacttcc ttcggcggga
      241 gtttcagcac gcgcggcact ttggtccgga agtcgccgcc gggaatctgc tccaggcact
      301 gccgcaaaat gcggatcgac tgcttgattt ccgaaatccg cacgaagtag cggttccaac
      361 tgtcaccgac cgtcacgccc ttgggtatgc cgttcgagcc gccgtccact ccaaccggta
      421 tatcaaagtc gaaccggtca taaatgtcat acggctcgca cttgcgcagg tcccatttga
      481 cgccgctgcc acgcaggcac ggcccggtca ggccccaggc gatcgcgtct tcggccgaga
      541 ttacccccac gccggccgtc cgttttacga atatgtggtt gtacgtcagc aggttgttgt
      601 attcgtcgat cttcggctcg aggtaatcga ggtaccaggc cagtttctcg aggaagcctt
      661 caggcaaatc ctggttcggg ccgccgatcg tgatatagct ataggtcagc cgcgccccgc
      721 aggtcgcctc gaacatgtcg aggatgtact cacgctcgcg gaatccgaaa agcaacggag
      781 taaaggcgcc catttccagg ccgaacgtcc cgaacgaaac caggtgcgac gcaattcggt
      841 tcagctcggc catgatgatg cggatgtagc gcccgcgctc gggcacttcc agcccggcca
      901 gcttctccac ggcgatgctg aaggcctggt tgttgttcat cgccgccagg tagtccaggc
      961 ggtcggtgat ggggatgaac tggaacggcg cgacgttttc gccgaccttc tccgcgcagc
     1021 ggtgcagata gcccatgtgc ggcgtcactt ccagcaccat ctcgccgtcg gtccgcagga
     1081 ccaggcgcaa cacgccgtgc gtgctggggt gctgcgggcc catgttgatc agcatttcct
     1141 cggtgcgcag ttcgccgggc aggctggcgg agtccagatc ggcgagcagg tcgggagcgg
     1201 cggtgtaggt cttttctggc atatgtctac actttggcga cgagaccgcg tgataaggtg
     1261 ctaacctgat caggtaatag cgggctggcg gtcgcccgaa aacaggacac tgtaaccggc
     1321 aggtcttgct gactttgtga cttcttcact tggtcacctc ggctgtgnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ttcgctttgc cgagctgcat ggagacgacg
     1501 tcgtacacca ggtcgatgta cggctccgga tggcgatcga tcccgctgat gcaccgcagc
     1561 atgttcatcg ccaggtgcgg ttcatcgcgg aggaaaaggg ccacctcgtg ccagcgctcg
     1621 gcggcgacgg tcacttgcgg atgcagcgcg tccgccgcta cctcagtaat cgcatcgccg
     1681 aatcgctgct tcagaatctc tgctatttcc tgtggtgtca cagtatgttc caatcacagg
     1741 ggcggctctg ccggcccggt ccagtcacgc cccgcgttca cactgcctcg gcctgcttgg
     1801 cgatggtttg ctgccgcatc ttgtcctgca gacggagcaa accctcgacc agcgcctccg
     1861 ggcgcggcgg gcagcccggc acgtagacgt ccaccggcac gatcaggtcg acgcctttga
     1921 ccacgtgata accgtatttg aagtaggggc cgccgccgat cgcgcaggcg cccatcgcca
     1981 gcacgtactt cggatcgggc atctggttgt acaggcgccg gatgcgcgag gccattttga
     2041 aggtcacggt gccggccacg atcatcagat ccgattggcg cggggtagcg cggtaggcac
     2101 cggccccgaa gcggtccagg tcccagcgcg acgaggcggt cgccatcatc tcaatggcgc
     2161 agcacgccag gccaaaggtc atcggccaga tgctgttggc gcgggcccag ttgagggccc
     2221 aatccaccgt ggttatggcg aagttttctt cgaagcggtt ttgcagccag cccatattgt
     2281 ttacgtccta gcctgcgcgg gcgcctccgc gaactcgttc accgtttggc gtaaacgcgc
     2341 atcgtccagc gtcctggttc cgttttcggt ggtcggcggc cgtgcctcgg ctcgcgccgt
     2401 tctggtggtc cgcacccaat ccaggtatcc gaaccgccac aggaagagga agccgaccac
     2461 gatcacgccg aagaagaaca acatgtccca catggcggcc cagcggatcg actgcagcgc
     2521 gtcggcggtt gcggcgcctc ccgcctcggt tgtgcctccg ccgccaaaaa cgacggccca
     2581 cgggtagaac agggccagtt ccacgtcgaa aaccaggaag accagcgcca ccacgtaaaa
     2641 ccgcaaatcg aactgcaccc accccggccc gagcggcagt tcgccgcatt cgtaagcggt
     2701 gatcttgtcc gggttcgggc gattcgggcg cagcagccgt ccgaccagca gtgcgccgaa
     2761 gccgaagaag aaggcggcta ccgcgaaaat cgccaggatt tgcagcaggt ccgtgccgcc
     2821 ctcagccaat agcgatgcgt gtgcaaagac cattatgccg ttccgtcact cgaatttgga
     2881 agcatacccg ggcacgccct ccgtaactcg ccgtagttca gcgtcgtagc aagacacata
     2941 ccgttgggtc tcactgccgt tggtccatca ttcggccggc ggaggcgccg gcttggccgg
     3001 tggctcggcc gctggcttcg cagccggagg cggcttcgcg accggctgct ccgtcagagc
     3061 cttgagcatt tcctcacgtc gcggctcggc gcgccgctgc caagcctctt tgagagcttg
     3121 agcccaggcc ggcaggcgtt tcttcttcat ccacagcggc agcggtgatt ggagtccctg
     3181 ccgggcgagt tcggtgaact ccacgaccgt gtcctggcgg gtgaagccgg aaatgtcgtg
     3241 caggtcgccc atgtggatgc agtcggtggg gcagttttcg gtgcacaggc cgcagaacat
     3301 gcacttggaa taatcgatcg cgtagcgggt catggcgccg ccgcgcgaaa tgccggtttc
     3361 cttctcgatt ttccgcggcc cggatttttc gatgtagatg caatctacgg ggcaggcccg
     3421 ggcacagttg ccgcaggcgc tgcatcgttc gatttcgtac caatggaacc cgcgaaatcg
     3481 cggtttaacc gtgggcggca cgtcgggata ctgcaccgtg acggtccgcg cgaagcagta
     3541 gcgcagcgtg attcccatcc cgatcaggat cgtccgcact gcctccaagc tgtctcttat
     3601 gtgttttctc ataaagctct gattcgaaac cctctcgtca tcgctcggaa aaccgcggcc
     3661 gaaagctagc cggccaaagg tcggacctgg aggccgacat tctataccat gaaaccctta
     3721 ttaatgccac gcgcgccgcg aaggcctgcg cccgccatca agcatgcaaa cggcgtgccc
     3781 acccgcccct cggctgccca cccgcgtacc atgtcgtgcc atgcccaagc gtgggtg
//
LOCUS       DAOSYS010000311         3824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19246_length_3824_cov_2.619528, whole genome shotgun sequence.
ACCESSION   DAOSYS010000311 DAOSYS010000000
VERSION     DAOSYS010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19246_length_3824_cov_2.619528"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1449)
                     /locus_tag="PLU99_14425"
     CDS             complement(238..1449)
                     /locus_tag="PLU99_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18287.1"
                     /translation="MTPPRVLLLTGTLPSESDVGGLILRDLVRLYPPSRLCCCGLLNS
                     VAARPSVDPGWLPTLALRAPRGLLARRRGGLAERAVTHARFRIARRLHRPNLVRRVVA
                     FAREHQVELVWAVLNQPVTYGVAPAVADCLNVPLVVTVWDPPDSVCLNANLDRFSTAL
                     AVRDFERALGRASRCGVISEAMQREYEARYECHTVVMRHAVRADECTRPARCPNSQGP
                     FTIGFCGSLYAVREFRALLAALDAAGWQIAGRPVRLRMVSAAVSGRIGPNAWVEWLGW
                     RPQREVIRILSESDVNYLPYWFDMQRRNAVRLCFPSKLTTYLAAGRPVLFHGPDDAAP
                     AELMRRHPIGVCCHSLAGAGLVAALSQSVSLDLYARAAEAIERLVASEFNAANLPGRL
                     AQLLGASEPDE"
     gene            complement(1478..2536)
                     /locus_tag="PLU99_14430"
     CDS             complement(1478..2536)
                     /locus_tag="PLU99_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO18288.1"
                     /translation="MRMLVTGGLGTIGAGLVAELRRRGRYVVSCDLYHQPDEVGFSLR
                     TDVAQPLYARCDVSEYRQLERVFDRMGPFDYVYHCAAEFGRWNGEDFYETLWRTNCIG
                     TKNIIRLQEQRGFRLIHFSSSEVYGDWPNLMVETVMEEYEVKQLNDYAMTKWVNEMQV
                     RNSRQQFGTETVVVRLFNTYGPGEYYSPYRSVNSRFLFCGLRGLPWTVFRGHARTSTY
                     LADTVRTLANISENFRPGETYNIGGCELHSIEELSDIVLKITGADPKLVQYRDSEILT
                     TLVKRVDVSKSVRDLDHRNSYSLEAGMRLTADWMREVYRMPRKDTDVAPTDDAVRPPA
                     VVTTRRSVLCGAGVGSLP"
     gene            complement(2556..3269)
                     /locus_tag="PLU99_14435"
     CDS             complement(2556..3269)
                     /locus_tag="PLU99_14435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOO18289.1"
                     /translation="MWACDARRDPELLARCYAASPQGYWTNLRNDERMHARIAALLER
                     FAGGRQLCDVGCGDGRLLMALRTRWRVHGLEAAPAAVKLCRAKDLDVEFGTPANSDRV
                     GAYDAVTCVDTLEHMLDPVAELSGMAAMLRAGGVLLAITGDARSWTARLSGAAWEYLH
                     CVGHISVLSRAGLCRMLTGAGLSVLTQRGLGHSASVGIRAWAAVWLGNLWRRFRGYRS
                     RRVPYCHDHQLVIARKPAG"
     gene            complement(3486..>3824)
                     /locus_tag="PLU99_14440"
     CDS             complement(3486..>3824)
                     /locus_tag="PLU99_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOO18290.1"
                     /translation="ALLFSERCRDVLIEGGRRGLTAADKEWSSVRCRPITIRSKAWIG
                     MRVIILPGVTIGEGCIVGAGSVVTRDLPAWTVSAGNPARVLRSLPAMGREIAEQAVSD
                     RTKEQLVEIT"
BASE COUNT          751 a         1320 c         1098 g          655 t
ORIGIN      
        1 tttaccgacc cgttcaaaca ctgcgctcga tgatccgcgc gcgggcaaca agccaacatc
       61 ggcgcttcct cctgacggcg cggacggcat ccggcgccgc acaaaccatt ctagtcctaa
      121 tacaacggta atatggccat cgccgagccc gcccgttgag gtgataaggt gatagcgtga
      181 taaagtgata aaggataatc cgcgaatccc tttatcactc tatcacttat cactttatca
      241 ctcgtccggc tcggaggcgc ccagcaattg cgcaaggcgc ccgggcagat tcgccgcgtt
      301 gaactcactt gcgacaagtc gctcgattgc ctcagcagcg cgtgcataca gatctagcga
      361 gacgctttgc gacaaggccg caaccaagcc cgcgcccgcc agtgagtgac aacaaacccc
      421 gatgggatga cgccgcataa gctcagccgg tgccgcgtcg tccggcccat gaaacaaaac
      481 cggccgcccg gccgcaaggt acgtcgtgag cttggatggg aaacagaggc gcacggcatt
      541 ccgccgctgc atatcgaacc aatagggaag gtagttcaca tccgattcac tcagaatgcg
      601 tattacctcg cgctgcggtc gccacccgag ccactcgacc caagcgtttg gcccgatcct
      661 cccgctgacg gccgcactca ccattcgcaa ccgcacggga cggccggcga tctgccagcc
      721 cgcggcgtcg agcgccgcca ggagcgctct aaactcgcgc acggcgtaaa ggctgccaca
      781 gaaaccgatc gtgaacggcc cctgcgaatt gggacaccgc gccggccgag tacattcgtc
      841 cgcgcgcact gcgtgccgca tgaccacagt gtggcattcg taacgcgctt cgtattcgcg
      901 ttgcatcgcc tcactgataa cgccgcaacg actagcgcgg cccagggccc gctcgaaatc
      961 gcgaactgcg agcgcggtac tgaaccggtc caggttggcg ttcaggcaga cgctatccgg
     1021 tggatcccac acggtcacga cgagcggcac gttcagacaa tccgcgacgg ccggcgccac
     1081 gccgtatgta accggctggt tcagcaccgc ccacaccagc tccacctggt gctctcgcgc
     1141 gaacgcgacc acccggcgga ccagattagg ccggtgcaga cggcgcgcta ttcgaaatct
     1201 ggcatgcgtc acagcgcgtt ctgccaggcc gccgcgacgc ctcgcaagca agccgcgggg
     1261 cgcccgcagc gctaaggtcg gcagccagcc tggatcgacg cttggccggg cggcgaccga
     1321 atttagcaaa ccgcagcagc acagccggct gggcggataa agccgaacca gatcgcgcag
     1381 gatcaggccg cccacgtcgc tctctgacgg caacgtgccg gtcaacaaca gaactcgtgg
     1441 cggcgtcaca accgaatctc gcctccgctg aacgcggtca aggcagggaa cccacaccgg
     1501 cgccgcacag aaccgagcgg cgcgtagtca cgacagcggg cgggcgcacg gcgtcgtctg
     1561 tcggcgctac atccgtgtcc ttgcgcggca tacgatacac ctcccgcatc cagtcggccg
     1621 tcaggcgcat cccggcctcc aggctgtaac tgttgcggtg atcgaggtcg cgtacagact
     1681 tgctgacatc cacccgtttg acaagcgtgg tgagtatttc gctgtcccgg tattgcacca
     1741 gcttcggatc ggcccccgta atcttcagta cgatatctga cagctcctca atcgagtgca
     1801 gttcgcagcc gccaatgttg taggtctcgc ccggccggaa gttctcgctg atgttggcca
     1861 gcgtgcgcac agtatcggcc aggtaggtcg aggtgcgcgc gtgcccgcga aataccgtcc
     1921 acggcagccc gcgcagcccg cagaacagga accggctgtt cactgagcgg tacgggctgt
     1981 agtactcacc cggaccgtag gtattaaata gtcgcacgac caccgtttcg gttccgaact
     2041 gctggcgcga gttgcgcacc tgcatttcgt tgacccactt ggtcatggcg tagtcgttga
     2101 gctgttttac ttcgtattcc tccatgactg tctcgaccat caggttgggc cagtcgccgt
     2161 agacctccga cgaggagaag tggatcaagc ggaagccgcg ctgctcctgc agccgtatga
     2221 tgtttttggt gccgatgcag ttcgtgcgcc acagcgtctc gtagaagtcc tcgccgttcc
     2281 agcgtccaaa ctcggccgcg caatgataaa cgtagtcgaa gggccccatt cgatcgaaga
     2341 cgcgctccag ttggcgatat tcgctcacat cacaacgcgc gtacagcggc tgcgccacgt
     2401 cggtccgcaa actgaagccc acctcgtccg gctggtggta caaatcgcac gaaaccacgt
     2461 agcgcccacg ccgcctcagc tccgcgacta aacccgcacc aatggtaccc aatccgcctg
     2521 taaccagcat acgcataact gtctccctgc cggtctcagc cggccggctt cctggcaatg
     2581 accaactgat gatcgtggca atagggcacg cgacgactgc ggtagccacg aaagcggcgc
     2641 cagaggttcc cgagccaaac cgctgcccag gcgcgaattc cgacgctggc cgaatgcccc
     2701 agaccgcgct gcgttagaac tgacaggccc gcgccggtca gcatgcggca caggcccgcg
     2761 cggctcagca ccgaaatgtg gcctacacag tggaggtatt cccaggccgc tcccgagagg
     2821 cgcgctgtcc aggagcgcgc gtcgccggta atagcaagca aaactccgcc tgctcggagc
     2881 atggccgcca tgccggacaa ttccgccacc gggtccagca tgtgctccag cgtgtcgacg
     2941 cacgtgacgg cgtcataagc tccaactcga tcgctgttgg ccggcgtacc aaattcgacg
     3001 tctaggtcct ttgcgcggca caatttcacg gccgccggcg cggcttccag gccgtgcacc
     3061 cgccagcgcg tccgcagcgc catgagcagt ctcccgtccc cgcagcccac gtcgcagagc
     3121 tggcgtcccc cggcgaaacg ctcgagtagc gcagcgattc gcgcatgcat tcgctcgtca
     3181 ttccgcaggt tagtccagta cccctgcggc gacgcggcat aacagcgcgc caacaactca
     3241 gggtcccgcc gggcatcgca ggcccacacc gagccgcacc tccggcattt tcggtacttt
     3301 agggccggca gtctcgtccg caattcctca gaaaagaacg gttccgccag gcggctagta
     3361 tcgatttcgg cgtaaggcgc gagctctgca gcacatgcgg ggcacgtgcg caaggcgtgc
     3421 atcgcgacct cttgccgcgt tacgcgcctt ttctcacacc gctcttgccg gacagcacac
     3481 tgcattcagg ttatctcgac taactgctcc ttggtgcgat ctgacactgc ctgctcggcg
     3541 atctcgcgcc ccatggcggg taagctgcga agcacccgtg ccgggttgcc tgccgatacc
     3601 gtccacgccg gcaagtcgcg tgtgacgaca ctgcccgccc caacgatgca cccttcccca
     3661 attgtcacgc ccggcaagat tatgacgcgc atcccaatcc aggccttgct gcgaatggtg
     3721 atcggccggc accttaccga ggaccactcc ttgtcggccg cggtcagccc ccgccgtcct
     3781 ccttcaatca gcacgtcgcg gcagcgctcg ctgaagagca aggc
//
LOCUS       DAOSYS010000312         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19391_length_3803_cov_5.149680, whole genome shotgun sequence.
ACCESSION   DAOSYS010000312 DAOSYS010000000
VERSION     DAOSYS010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19391_length_3803_cov_5.149680"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PLU99_14445"
     CDS             <1..1153
                     /locus_tag="PLU99_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18291.1"
                     /translation="EECMRWCYYETNLLGDPSLLVLGANRPLKIKLQGTLPTIIPPNE
                     PTTITVLVDPGDEAYVPETAALNYRVNDGTFESVPLVQIDGELYQGTLPPTACDSTLE
                     YYFSADGTESGTVNLPALAPVDMFRAYVATYVSFLNDDFETDLGWTVTNDASLTSGMW
                     QRDVPMAIAPEHHNPPDADFDGSGRCYVTERRYGCDVDFGPTILTSPMFDFSNSTNPM
                     IRYARWIYCDDARPEDEWYHPEHFDYLDVQLSNDGGLTWVPVEHEVSIGGWVQREIRV
                     RDYVALTGRVQIRFSVEDQFNNSVTEAAVDGVVIYDLHCDAQPWQLGDLNCDGAVNVF
                     DIDPFVLALTDMAGYAAAYPSCDAMLADVDDDGTVNVFDIDPFVTLLTQ"
     gene            complement(1251..1817)
                     /locus_tag="PLU99_14450"
     CDS             complement(1251..1817)
                     /locus_tag="PLU99_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18292.1"
                     /translation="MAWLTWFALESTLALAAALFTANFALLVMWRRTGRGRPLLAGLA
                     VSILLLAIQGFVVTRREHAGRLLRTIERDLLTAQTTGLSAALADDFRTARMDKGQFLD
                     FVARQYGRIRVSSLHCSQLEVRDSQPDQFTVEAAYQADLRGDFMGGWVRSRWELTFKR
                     TADGWRIAFLRPIHIDGVGQPDLRALEP"
     gene            1949..2620
                     /gene="tmk"
                     /locus_tag="PLU99_14455"
     CDS             1949..2620
                     /gene="tmk"
                     /locus_tag="PLU99_14455"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOO18293.1"
                     /translation="MSGKFIVVDGPDGAGKGTQIVNLARWIESEGGNCVRTRDPGGTT
                     VGDRIRHILLDYDLSDMDATCETLLFMASRAQLVAEVVRPALAAGRTVICDRFISATC
                     AYQVAAGLPREKVLELGRLAVGQTWPDLTLVLDLPPEVGFERIGRKPGQTGRRGGGGQ
                     VSLFEGTSPDAMERRPMEFHRRVRALFRELPQVYPAPVVLVDAAGSEEAVFAEIQEAI
                     RRAFV"
     gene            2613..>3803
                     /gene="holB"
                     /locus_tag="PLU99_14460"
     CDS             2613..>3803
                     /gene="holB"
                     /locus_tag="PLU99_14460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOO18294.1"
                     /translation="MSDVQHQPRAISILRRSLSSGRTHHAYLFEGPEGVGKQMAAKAL
                     AARLLCLSPDIPADADACGNCASCRLLARGNHPDFHLIERRLHKLHPDRTIRAGKGLF
                     LVVDVIRTFLIEPAGTKPAVSSRRVFLVDEAERMNEGAQNALLKTLEEPPGQTCLILV
                     TSSAERLLPTIRSRCQRVAFGLLPPEFVARQLGQRLNLPAADAEALARLAGGRLGAAL
                     RWHRAGLLDAAQEAAQSIARLERGGVTRFSQELIEAGTALATRLAQADSQQEHHVEGE
                     DAEDSGASEGGGDEIIDEEAVAVSGRRGRASALGTSELRDALKLVLMLVAAVYRDALL
                     LNVGGPAALHLAAQRPLTESISQRTSAAHLDTCIRAVAHAERMLDRNVTPALVGEFLG
                     AALVG"
BASE COUNT          673 a         1142 c         1282 g          706 t
ORIGIN      
        1 cgaggagtgc atgcgctggt gttactatga aacgaacctg ctgggcgacc cgagtttgct
       61 ggtcctcggc gccaatcggc cattgaaaat caagctccaa ggcacgctcc cgaccatcat
      121 cccgcccaat gaacctacca cgatcacggt actggttgat cctggcgatg aagcttatgt
      181 gcccgagacg gctgcgctga actaccgggt caacgacggt actttcgaat ccgtaccgct
      241 ggtgcaaatc gacggcgagc tttatcaagg cacgctgccg ccgaccgcct gtgactcgac
      301 gctcgagtat tatttcagtg cggacggaac cgaaagcggc acggtgaacc tgccggcgct
      361 ggccccggtg gatatgttcc gggcctacgt ggcgacgtac gtgagcttcc tcaacgacga
      421 ctttgaaacg gacctgggtt ggaccgtcac gaacgacgcg agcctgacaa gcggcatgtg
      481 gcagcgggac gtcccgatgg cgatcgcccc cgagcaccac aacccgccgg acgccgattt
      541 cgacggctcc ggcaggtgct atgtcaccga aagaaggtac ggttgcgacg tggactttgg
      601 cccgaccatt ctgacctcgc cgatgttcga cttctccaat tcgaccaacc ccatgatccg
      661 ctacgcccgc tggatttatt gcgatgatgc ccgtcccgag gacgaatggt accatcccga
      721 acacttcgac tatctggatg tgcagctctc aaacgacggc ggcctcactt gggtgccggt
      781 cgagcacgag gtcagcattg gcggatgggt ccagcgagaa atccgcgtca gggactatgt
      841 agcccttacc gggcgggtcc agatacgctt ctcggtcgaa gaccaattca ataactcggt
      901 taccgaagcg gccgtggacg gggtcgtgat ttatgacctg cactgcgatg cgcaaccctg
      961 gcagctcggc gatttgaatt gcgacggcgc ggttaacgtt ttcgacattg acccgttcgt
     1021 gttggcgctg accgatatgg ccggttatgc ggcggcatac ccgagctgcg acgccatgct
     1081 ggccgacgtc gacgacgacg gcacggtcaa cgtgtttgac atagatccgt tcgtcacgct
     1141 gctgacgcag tgatttagcc cgacgacggc gggaatcgcc ctttgagggc cgcctgattc
     1201 tcgtgcggag cagcgggaga ttttccgctg ctccgccgtt tctttctggg tcacggctcc
     1261 agggcgcgca ggtctggttg cccgacgccg tcgatatgaa tgggacgcag aaacgcaatg
     1321 cgccacccgt ccgcagtgcg cttgaaggtc agttcccagc gcgaacgtac ccagccgccc
     1381 ataaaatcgc cgcgcaagtc ggcctggtac gccgcttcga ccgtaaattg gtctggttgg
     1441 ctgtcgcgaa cctcgagttg gctgcagtgc aggctggaaa ctcgtatccg cccatattgg
     1501 cgggccacga agtcaagaaa ctgccccttg tccatccggg ccgtccgaaa atcgtccgcc
     1561 agcgccgccg acaggccggt ggtttgcgcc gtcaacagat cgcgctcgat cgttcgcaaa
     1621 agacgacctg cgtgttcccg gcgcgtaaca acgaagcctt gaatggctaa caacagaatc
     1681 gaaaccgcca aaccggccag cagcggtcgc ccccgtccag tccgtcgcca catcaccagt
     1741 aaagcaaaat tggccgtgaa tagcgcagcc gcaagggcga gtgtggattc caaggcgaac
     1801 caggtcaacc aagccatgcc atgctcccgg actctgcgga cacgctcggg ttggcaaggt
     1861 aaccgcggcg gagggggccc gccagcgccg gatgcgctga cattcggttg ccggccgcgc
     1921 gtgcatgcac tatcgtcgca ttatgaatgt gagtgggaaa ttcatagtcg tcgacgggcc
     1981 ggacggcgcg ggcaagggca cccaaatcgt caatctggcg cgctggatcg agagtgaagg
     2041 cgggaactgc gtccgcaccc gcgatccggg cgggaccacc gtcggcgatc gcatccggca
     2101 tattctgctt gactacgatc tgagcgacat ggatgcgact tgtgagacgc tgctgttcat
     2161 ggcctcccgc gcccagcttg tcgcggaggt ggtgcggccg gcgctggcgg cgggccggac
     2221 ggttatctgt gatcgcttca tttccgctac ctgcgcgtac caggtggcgg ccggcctgcc
     2281 gcgagagaag gtgctggaac ttgggcggct ggccgtcggt cagacgtggc cggatttgac
     2341 gctcgtgctc gatctgccgc cggaggtcgg attcgagcgc atcgggcgca agccgggcca
     2401 gaccgggcgc agaggcggcg gcggacaggt gagcttgttc gagggcacgt cacccgacgc
     2461 gatggagcgg cggccgatgg agtttcatcg gcgtgtgcgg gcattgtttc gcgagttgcc
     2521 gcaggtttat ccggcgcccg tggtgttagt ggatgcggct ggcagtgagg aggccgtctt
     2581 cgctgaaatt caggaggcca tacggcgtgc gtttgtctga cgtacagcac cagccgcgcg
     2641 cgatttcgat tttgcgccgc tcgctgagca gcgggcgcac gcatcacgca tacctgttcg
     2701 aggggccgga gggggtgggc aagcagatgg cggccaaggc gctcgcggcc cggctgctgt
     2761 gtttgtcgcc ggacattccg gcggacgccg acgcgtgcgg gaattgcgct tcgtgccggc
     2821 tgctggcgcg tggcaatcat ccggactttc acctgattga gcgccgcctg cacaaattgc
     2881 acccggaccg caccatccgc gccggcaagg ggctgttcct ggtcgtggac gtaattcgca
     2941 cgttcttgat agagccggcc ggcaccaagc cggcggtcag ctcgcggcgc gtgttcctgg
     3001 tggatgaggc ggagcgtatg aacgaggggg cccagaacgc gttgctcaag acgctggaag
     3061 agccgcccgg acagacttgc ctgattctgg ttacttcttc ggccgaacgg ttgctgccca
     3121 ctattcgctc gcgctgtcag cgcgtggcgt tcgggctgct gccgccggag ttcgtggcgc
     3181 ggcagttggg ccagcggctg aacctgccgg cggcagacgc cgaagcgctg gcgcggctgg
     3241 ccggcgggcg gctgggggcg gcgctgcgct ggcaccgggc ggggctgttg gatgccgccc
     3301 aagaggcagc ccaaagtatc gcacgcttgg agcgcggcgg cgttacccgg ttcagccagg
     3361 agttgattga ggctggcact gcactggcga cccggctggc gcaggcggat tcgcagcagg
     3421 agcatcacgt cgagggtgag gacgcggagg attccggggc aagcgagggc ggcggggacg
     3481 aaataataga tgaggaagcc gttgcggttt cgggccgccg tggtcgtgca tccgctttgg
     3541 gaaccagtga gctgcgcgac gcgctgaagc tggtgctgat gctggtcgcg gccgtctacc
     3601 gggatgcctt gctattgaac gttggaggac cagcggcgtt gcacctggca gcgcagcggc
     3661 cgctgacaga gtccatctcg caacggacat ccgcggctca cctggatacc tgcattcgtg
     3721 ctgtggccca cgccgagcgc atgttggatc gcaatgttac gccggcgcta gtgggcgaat
     3781 ttctcggtgc ggcactggtt ggg
//
LOCUS       DAOSYS010000313         3800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19410_length_3800_cov_5.038184, whole genome shotgun sequence.
ACCESSION   DAOSYS010000313 DAOSYS010000000
VERSION     DAOSYS010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19410_length_3800_cov_5.038184"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLU99_14465"
     CDS             <1..352
                     /locus_tag="PLU99_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOO18295.1"
                     /translation="RFHLWGMGAQWVHEYGDPGKAEEFAWLRAYSPYHNVRDGVQYPA
                     VLLVTAESDNRVDTAHAFKMAARLQAATASDRPVLLRVEQKAGHGAGKPLSMRIENES
                     EDWCFLMWQLGMVE"
     gene            476..1012
                     /locus_tag="PLU99_14470"
     CDS             476..1012
                     /locus_tag="PLU99_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOO18296.1"
                     /translation="MPILPLALALLLLWPLAGCVGRRSLEVVDYVDLERYMGKWYEIA
                     RYPASFERDCVASTAEYTLRDDCKVEVVNSCRVCAFEGRERSIRGIARVVDPETNARL
                     KVAFFWPFEGDYYLIMLDDEYRWAVVGEPRRRYLWILSRTPQLEASTYEAILARLPAL
                     GYDPSRLQCTPQLAGVGG"
     gene            1094..2581
                     /locus_tag="PLU99_14475"
     CDS             1094..2581
                     /locus_tag="PLU99_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOO18297.1"
                     /translation="MFVVVILVLSIVLAFPAPLYRWVTDTPAVVAWAVAATLVPVAVA
                     ALAARQALRRLERFPEDPNRGQSVLTHGMRAVHGTLAIGQSGLLLCTNWLLLVARTPV
                     VGDWPVIPALLAISPFLVSALLVWVVSYPADRAVRQIALEAFLFRGRPVQPVWSLRQY
                     VINNVRHQILFILIPMLLIVAARDVIVIYEEPLRRFSGHPYTADLLLGAAAALVALIA
                     PEILRYVWSTRRLPDGPLRDRLTQVCRKLRIRYREILVWRAGGGVVNAAVMGVVAPLR
                     YILITDGMLEQLDDAKIEAVFGHEAGHVKRHHIFFFLLFAVITGCLVTLFSIHSRHMN
                     SREQQMVGTLLAVALLFKWSVVFTWISRRFERQADMFGARVLALCGLPCVQPCELHRP
                     EHSNPGPDPLGDALCSTAANVFSNTLNEVAVLNGMPPEGRSWRHGSISSRSRTLLRLA
                     ADPAAVASAERTVARIKIVITVVAVICTAWAGWELKLWELLKHWG"
     gene            2651..3454
                     /locus_tag="PLU99_14480"
     CDS             2651..3454
                     /locus_tag="PLU99_14480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOO18298.1"
                     /translation="MLVDTHCHLTFGDLAPQVGAVLQRAQEAGVARVITVSTSAAEIG
                     TSVEILEQFSNVCLVAGIHPHEAGHVTSEDLSALAALLCGQWAGADGRRVVGIGETGL
                     DFHYDFAPRPQQEQVFRAHLELACRTQKPVVIHARESEERVCDIIAEYEQLRGRVVFH
                     CYSAGVATARRILDLGDFVSFTGVVTFKNAGTIRESARFVPLDRVMLETDAPYLSPEP
                     VRKIRPNEPALLVHTARFIAELRGEPLEKFAAATTANAEKFFGLPEVAE"
     gene            3451..>3800
                     /locus_tag="PLU99_14485"
     CDS             3451..>3800
                     /locus_tag="PLU99_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOO18299.1"
                     /translation="MSTYLVTGGAGFIGSHVVRGLLKRGAAVRVLDNLSTGKRANLAE
                     VERDIELVEGDIRDQETVKRCMNGVAGVLHLAARASVPRSVAHPFEANDVNVTGTLTL
                     LLAARDAGVRRFVYS"
BASE COUNT          598 a         1072 c         1368 g          762 t
ORIGIN      
        1 gcgttttcat ctatggggca tgggagcgca gtgggtacac gagtacggcg atccgggaaa
       61 agcggaggaa ttcgcgtggc tgcgggcgta ctcgccatat cacaacgtgc gcgatggggt
      121 ccagtatccg gcggtcctgc tggtgacggc tgagtcggac aaccgcgtgg atacggcgca
      181 tgccttcaag atggcggcgc gtttgcaagc cgcgaccgca agcgaccggc ccgttctcct
      241 gcgcgtcgag caaaaagcgg ggcacggggc gggtaagccg ctgagcatgc gtattgagaa
      301 cgagagcgag gattggtgct ttctgatgtg gcaattgggg atggtcgagt agggggctga
      361 accgcataac tgagccgcgg cccggcagag gcttgccccg ccggcacttc gggcgtgaag
      421 cacgcgcgcc gactatgatc cctcgcgcag gcgcgtgttt tgggaaggag cacttatgcc
      481 gatattgccg ctggcgctcg cgttattgct gttgtggccg ctggcggggt gtgtcggccg
      541 gcggtcgttg gaggtcgtgg attacgtcga cctggaacgg tacatgggga agtggtatga
      601 gatcgcgcgc tatccggcgt cgtttgagcg agattgcgtg gcctcgaccg ccgagtacac
      661 gctgcgggac gactgcaaag tcgaggtcgt gaattcatgt cgcgtctgcg cctttgaggg
      721 gcgggaacgc tcgattcgag gcatcgcgcg cgtggtcgat ccggaaacga acgcccgcct
      781 gaaggtggcc tttttctggc cgttcgaggg cgattactac ctcatcatgc tcgacgacga
      841 gtatcgctgg gcagtggtgg gcgagccgcg gcggcggtat ctctggattc tcagccgcac
      901 gccgcaactc gaggcgtcca cgtacgaagc gattctcgcg cgactgccgg cgctgggata
      961 tgacccgagc cggttgcaat gcacgccgca gctcgcgggg gtcggcggtt aagcgtcgcg
     1021 gccacattcg agagccaggc tggtcccggg gctgcgcggc gggggccggc tttttcgcgt
     1081 atcattcagg catatgttcg tagtcgtcat tctggttctt tccattgttt tggcgttccc
     1141 cgctccgctt taccgctggg tgaccgacac gccggcggtt gtggcttggg cggtggcggc
     1201 gacgctggtg ccggtcgcgg tcgcagcttt ggccgcgcgt caggcgttgc ggcggctgga
     1261 gcgcttcccg gaggatccga accgcggtca gtcggtcctg acgcatggca tgcgcgccgt
     1321 tcacggcacg cttgccattg ggcaaagcgg gctgctgctt tgtacgaact ggttgctgct
     1381 ggtcgcgcgt acgccggtgg taggcgattg gccggtgata ccagcgctgc tggccatcag
     1441 tccgtttctg gtgagtgcgc tgctggtctg ggtggtgagt tatccggcgg atcgcgccgt
     1501 acggcagatt gccctggagg cgttcctgtt ccgggggcgt ccggttcagc cggtgtggtc
     1561 gctgcgccag tacgtcatca ataacgttcg gcaccaaatt ctgttcatac tgattccgat
     1621 gctgctgatc gtggcagcgc gggacgtgat cgttatttac gaagagccgc tgcgacggtt
     1681 ttcgggacac ccgtatacgg ctgatttgct gctgggggcg gcggccgcac tggtggcgtt
     1741 gatcgcgccg gagattctgc ggtacgtgtg gtcgacgcgg cggctgccgg acggcccgct
     1801 gcgcgatcgc ctgacgcaag tgtgccgtaa gctgcgcata cgttaccgcg agattctggt
     1861 atggcgggcg ggaggcgggg tcgtcaacgc ggcggtgatg ggtgtggtgg cgccgctgcg
     1921 ttacatcctg atcacggacg gcatgttgga gcagcttgac gacgcgaaga tcgaggcggt
     1981 tttcgggcac gaggcagggc acgtgaagcg gcaccatatc ttcttcttcc tgctgttcgc
     2041 cgtgatcacg ggttgcctgg ttacgttgtt ttccatccac tcccggcaca tgaactcgcg
     2101 tgagcagcag atggtgggca cgctgctggc cgtggcgctg ctgttcaagt ggagcgtggt
     2161 tttcacttgg atttccagac gcttcgaacg gcaggccgat atgtttgggg cgcgtgtgct
     2221 ggcgctttgc ggattgccgt gcgtccaacc ctgcgaattg caccggccgg aacactctaa
     2281 tcccggcccc gatccgctgg gcgacgccct gtgctccacc gcggccaacg tgttcagcaa
     2341 cacgttgaac gaggtggccg tgctgaacgg catgcctccg gagggccgga gttggcggca
     2401 cggttcgatc agctcacgtt cgcggacgct attgcggctg gcagccgatc cggcggccgt
     2461 ggccagtgcg gagcgaaccg tcgcgcgtat caagatcgtg atcacggtgg tggccgtgat
     2521 atgcacggcg tgggccggat gggagctgaa gctgtgggag ttgttgaagc actggggctg
     2581 agcgagcaga gccggggcgt gtcgccccgg gattatcacg gaaagcaacc tggcgcgtga
     2641 gcacagccga atgttagtcg atacacattg tcacttgacc ttcggtgatc tggcgccgca
     2701 agtcggagcg gtgctgcagc gcgcgcagga agcgggcgtg gcgcgcgtga taaccgtctc
     2761 aaccagcgcc gccgagatcg gcacgtccgt tgagattctg gaacaatttt cgaacgtgtg
     2821 cctggtggcc ggcattcatc cgcacgaagc ggggcatgtc acgagcgaag acctgtcagc
     2881 gctggcggcg ttgctctgcg gccagtgggc cggcgcggac gggcggcgcg tcgtgggaat
     2941 cggggagaca gggctggact ttcattacga tttcgcgccg cgtccgcaac aggaacaagt
     3001 gttccgggca caccttgaat tggcctgccg gacgcagaag ccggttgtga tccacgcgcg
     3061 cgagtcggaa gagcgcgttt gcgacatcat cgcggagtat gagcagctcc gcgggcgagt
     3121 tgtattccac tgctactcgg cgggcgtcgc gaccgcacgc cgcatcctgg acctgggcga
     3181 ctttgtgtcg ttcaccgggg tggtaacgtt caagaacgcc ggcacgattc gcgagtcggc
     3241 ccgctttgtc ccgctcgacc gcgttatgct cgaaaccgac gcaccctatc tatcgcccga
     3301 gccggtgcgt aagattcggc ccaacgaacc ggccctgttg gtacacacgg cccggttcat
     3361 cgccgagctg cgcggcgagc cgttggagaa gttcgccgcg gccacgaccg caaatgcgga
     3421 aaagtttttt ggcctgccgg aggttgcaga atgagcacgt atctggtcac tggtggagcg
     3481 ggtttcatcg gcagtcatgt cgtgcggggg ctgctgaagc gcggcgcggc ggtgcgcgtg
     3541 ctcgataatc tgagtaccgg caagcgcgcc aatctggcgg aggtcgaacg cgacattgag
     3601 ctggtcgagg gcgacattcg tgaccaggaa acggtgaagc gctgcatgaa cggcgtggcc
     3661 ggcgtgctgc atttggcggc gcgggccagc gtgccgcgta gcgtagcgca tccgttcgaa
     3721 gccaacgacg tgaacgtgac cggcacgctc acgctgctgt tggcggcgcg cgacgcgggc
     3781 gtgcggcgct tcgtctactc
//
LOCUS       DAOSYS010000314         3799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19418_length_3799_cov_5.112179, whole genome shotgun sequence.
ACCESSION   DAOSYS010000314 DAOSYS010000000
VERSION     DAOSYS010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19418_length_3799_cov_5.112179"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /gene="holA"
                     /locus_tag="PLU99_14490"
     CDS             <1..945
                     /gene="holA"
                     /locus_tag="PLU99_14490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOO18300.1"
                     /translation="EPHQKSVALARTLDRLLPLEADRQLAVTTYDASQSEEQGGPTFA
                     GVMDDLATLPFLTERRIVVVREADRFVGAYREKLERYLAAPSATGTLVLECRSFPKTT
                     RLYKACVGAGGQIEECRKLTGRALADFVTQKAQARGKRLDPGAAGRLCDLVGQDLGQL
                     AGEVEKLSLYVGQRPVITAQDVAELVGQTREEKIFRVMDCAGLGRVSEALTLWHQVLA
                     TDPDAVYKALGGIAFVIRRWIEAQQLRAEGASVAEIAPRMMMWGRQRELETLLRRLPL
                     GRLTELLAAIGELDSQAKSGRRSIETGVEELLLRVAAP"
     gene            1028..2278
                     /locus_tag="PLU99_14495"
     CDS             1028..2278
                     /locus_tag="PLU99_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18301.1"
                     /translation="MDARKVAPLVCAFTSLLAGCLDSNFGPTLYRPEIKPERPKPETA
                     VVQAEPEPPPIAPDEAELAAERVREYIAQVDTISQRAHRDLVTVSRAEDADPHELPHT
                     ATPHRAPPVEVVNVVEPPAEHVDPSPPQAEPAAPATTAVAPEPPEPVSAPVLAAVTAR
                     EQSAIAAPEPSGSLEAPSPNAPTAARHAPLSLTQFLQQWTGDPDDSSFRQQLDLRILR
                     AIAGDYEAAREPLTMVSAEQQAMGSRLIESLIAIREAHDGDPVQAIADVLANIDSLRE
                     ALRPMSELRIPVVTLCREVRGFGDYAPLEPATFLAGAAAEFVLYCEVQNFVSRPEEDK
                     FFHAKFEMRTAILNRAGETVLDIHDPDVVDRCRNRRTDCFIPRLVRLPGTLSPGEYVA
                     KVTVVDKLGEKLAENRASFRVVAR"
     gene            2398..2946
                     /locus_tag="PLU99_14500"
     CDS             2398..2946
                     /locus_tag="PLU99_14500"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOO18302.1"
                     /translation="MNFPRFIRRLIRAPYDGLRHFGIVEEGILYRCGQPKPQELAQLI
                     DQYGLKTVVSLRGTRGEQDPDSWEQAERSVCESHGAQFVAFPCNHKNPPTAEQVRQFL
                     KLTQDVSRRPVLVHCRLGQQRTLLFCGLYRVHSQGVDPAAALREMDELGFRSGIRRHR
                     RLLAAFHDLAAGLDGSGDSSAD"
     gene            complement(2983..>3799)
                     /locus_tag="PLU99_14505"
     CDS             complement(2983..>3799)
                     /locus_tag="PLU99_14505"
                     /inference="COORDINATES: protein motif:HMM:NF020672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HOO18303.1"
                     /translation="QALFEGQPAARLEEVIRAAEWQPLDDKRLSVRAGLRDEQEAAEM
                     CRRLMGAGKIRVIEAASLRLCVHNSHLQALSEDLQRRLKEFIAANPRLPGLARAQAPA
                     WMPGACPPRLRQALAEWWVSHGPVTLEHDHLVPAGKRQELPQVDQALLNAMLNEFDAG
                     AFQPPLIAELKCRTPKNEKRIRELIELSVAQGKLVRFAPDMWLHDRRWKELVERVTGA
                     IRERGGITVADIRTLLNSSRKYVVPIVEALDAAGITRRVGDLRQLGPKAPAGA"
BASE COUNT          662 a         1245 c         1248 g          644 t
ORIGIN      
        1 gagccgcatc agaagtccgt tgcgctggcg cggacgctgg accggctgct gccgctggag
       61 gccgatcgcc agttggccgt gaccacgtac gacgcctcgc aaagcgagga gcagggcgga
      121 ccgactttcg ccggcgtaat ggacgacctg gccacgctgc cgtttttgac ggagcggcgc
      181 attgtggtcg ttcgggaggc ggaccggttc gtcggcgcct atcgcgagaa gctggagcga
      241 tacctggccg caccctcggc aacggggacg ctcgtgctgg aatgccggtc gtttccgaag
      301 accacccgcc tgtacaaggc gtgcgtcggg gccggcgggc agattgagga gtgtcgcaag
      361 ctgacggggc gggccctggc ggacttcgtt acgcagaagg cccaggcgcg cggcaaacgc
      421 ctcgacccgg gcgcggccgg ccggctgtgc gacctggttg ggcaggattt ggggcagctc
      481 gcgggggaag tggaaaagct ctcgctgtac gtcgggcagc ggccggtgat tacggcccag
      541 gacgtggccg aactggtcgg gcaaactcgg gaggagaaga tattccgggt gatggactgc
      601 gctggactgg ggcgcgtgtc ggaagcgctc acgctctggc accaggtcct ggccacggat
      661 cccgacgccg tttacaaggc gctgggcggg atcgcatttg ttatccgacg ttggatcgag
      721 gcgcagcagt tgcgtgctga aggagcatcc gtagccgaaa tcgccccgcg catgatgatg
      781 tggggtcgtc agcgcgagct ggaaaccctg ttgcgccgct tgccgctggg ccgcttgacc
      841 gaattgctgg cggccatcgg cgaactggat tcacaagcca aatcggggag gcggtcgata
      901 gagaccggcg tggaagagct gctattacga gtggcagccc catagcgtcg gccccgcgag
      961 gtcgacgccg accgccttgg aattcgcgcg gcggaaaaat ttggaccggt aataaaggtg
     1021 ccttcgcatg gatgcgagaa aagtcgcgcc actggtctgc gcgttcactt cccttttggc
     1081 aggctgcctg gattcgaact tcgggccgac gctgtaccgg ccggaaataa agcccgagcg
     1141 gccgaagcct gagacggccg tcgtccaagc ggagccggag ccgccgccga ttgctcccga
     1201 cgaggcggaa ttggccgccg agcgagtccg cgagtacatc gcacaggtgg atacgatttc
     1261 gcagcgtgcc caccgggacc tggtgaccgt ctcacgcgct gaggacgctg acccgcacga
     1321 actgccgcac accgcgacgc cgcatcgcgc gccgcccgtc gaagtggtca acgtggtcga
     1381 gccccctgcc gaacatgtag acccttcgcc gccgcaagcc gaacccgctg caccggccac
     1441 aacggccgtc gcgcccgaac cgccggagcc ggtttcggcg cccgtgctgg ccgcagtcac
     1501 cgcgcgcgaa cagtctgcga tcgccgcgcc cgagccctcc ggatcgctcg aagctcccag
     1561 tccaaacgcc cccaccgccg cgcggcacgc accgctgtcg ctcacgcaat tcctgcagca
     1621 atggaccggc gacccggacg acagctcgtt ccggcagcag ctcgatctgc ggatcctgcg
     1681 cgctatcgcg ggcgactacg aagccgcgcg cgagcccctc acgatggttt ccgctgaaca
     1741 acaggccatg ggcagccggc tgatcgaaag cctgattgcc atccgcgagg cgcatgatgg
     1801 cgatcctgtg caggccatcg cggacgtgct ggccaacatc gacagcctgc gcgaggcgct
     1861 gcgcccaatg tccgagctgc gtatcccggt cgtcaccttg tgccgggagg tccgcggctt
     1921 cggtgattac gcgccgttgg aaccggcgac tttcctcgcc ggcgctgccg cggagttcgt
     1981 cctgtattgc gaggtacaga atttcgtcag ccggcccgag gaagacaagt tctttcacgc
     2041 caaattcgag atgcgaaccg ccattctcaa tcgcgccggc gagacggtgc tcgacatcca
     2101 cgacccggac gtggtggacc gatgccgcaa ccgccggacc gactgcttca ttccccggct
     2161 ggtgcggctg ccgggaacac tttcacccgg cgagtacgtg gcaaaagtca ccgtagtcga
     2221 taaactgggg gagaaactgg ccgagaaccg agccagcttc cgcgtggtgg cccggtagcg
     2281 aaaccgggct tgctgcggcg agtttgcgac gtgtcacccg cggatgagga gtgcagggca
     2341 tagggcggca cggcacgcat aggtcgccga atcttcaaaa ccaactgtgg gcggcggatg
     2401 aactttccgc gttttatcag acggctgatc cgggcgccgt atgacggcct gcgccacttt
     2461 ggaatagtcg aagagggcat actttaccgc tgtggccagc ccaagccgca ggagctggcc
     2521 cagctcatcg atcaatacgg gcttaagaca gtggtcagcc tgcgcgggac gcgcggcgag
     2581 caggatcccg attcatggga gcaggcggag cgatccgttt gtgagtcgca cggcgcccaa
     2641 ttcgtggcct ttccctgcaa ccacaaaaac ccgcccaccg ccgagcaagt gcggcaattt
     2701 ctgaagctga cgcaagacgt cagccgccgg cccgtcctgg tccactgccg gctcggtcag
     2761 caacgcaccc ttctgttctg tggtttgtac cgcgtgcatt cgcaaggcgt ggatcctgcg
     2821 gccgcgctgc gcgagatgga tgaattgggt ttccggtctg ggattcggcg acacaggcgc
     2881 ttgctggcgg cctttcacga cctggcggct ggactggacg gatcgggcga cagctcggcc
     2941 gattaattaa gtcggtaccg gaggctttcc gcacacgtca attcaggcgc cggccggtgc
     3001 ctttggcccc agttgccgca ggtcgccgac acgtcgcgta atgcccgcgg cgtccaaggc
     3061 ctccacaatc ggcacgacgt acttccgcga tgaattcagc aaggtgcgaa tgtccgcgac
     3121 tgtaattccg ccgcgctcgc gaatagcgcc ggtcacgcgc tccaccagtt ccttccaccg
     3181 ccggtcgtgc agccacatgt ccggcgcgaa gcgcaccaac ttgccttgcg ccacagatag
     3241 ttcaatcagt tcgcgaatgc gcttctcgtt cttcggcgta cggcacttca gctcggctat
     3301 cagcggcggc tgaaacgccc cggcgtcgaa ttcgttcaac attgcattga gcagggcctg
     3361 gtcgacttgc ggcagctcct gccgtttgcc cgccggaacg aggtggtcgt gctccaatgt
     3421 caccggcccg tggctcaccc accattcggc cagcgcttgc cgcagacgcg gcggacacgc
     3481 gccgggcatc cacgccggcg cctgggcgcg agccagcccc ggcagacgcg ggtttgccgc
     3541 gatgaattcc ttcagccggc gctgcaggtc ctcgctcaga gcctgcaagt gtgagttgtg
     3601 tacgcacagc cgcaacgacg ctgcttcgat cacacggatt ttgccggccc ccatcaaccg
     3661 ccggcacatc tccgctgctt cctgctcgtc gcgcagcccg gcgcggacgg acagacgctt
     3721 gtcgtccaaa ggctgccatt cggccgcgcg gatgacctct tccaagcgcg cggcgggctg
     3781 gccttcaaac aaggcctgc
//
LOCUS       DAOSYS010000315         3756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19698_length_3756_cov_5.801675, whole genome shotgun sequence.
ACCESSION   DAOSYS010000315 DAOSYS010000000
VERSION     DAOSYS010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19698_length_3756_cov_5.801675"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLU99_14510"
     CDS             complement(<1..887)
                     /locus_tag="PLU99_14510"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOO18304.1"
                     /translation="MKVERLVIIGSGPAGLTAAIYAARADCQPLVFEGFSAGGLIPGG
                     QLMFTTEVENYPGFPEGVLGPTLMKAMREQAARFGARFITEDVVAVNFRERPFYIKGD
                     ETEAWAHTVIVATGARANWLDLPNEQRLAKSGGGVSACAVCDGALPMYRDQEVIVVGG
                     GDSAIEEGSYLTKFAKKVYLVHRRDKLRASKAMQERFFKLVEEGKMAPIWDAVVTDVL
                     GDEMITGVRLRNLKTGKEWEMPIKGMFLAIGHTPNTQVFRDCLELDEKGYIRLTQPGR
                     TNTSVDGVFAAGDTRRGQSL"
     gene            complement(1492..1866)
                     /locus_tag="PLU99_14515"
     CDS             complement(1492..1866)
                     /locus_tag="PLU99_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO18305.1"
                     /translation="MNTCNESFRAAVEQAGGAYVIRLYGSAGMEHVDELRRRLLEVPC
                     AENARVVLDLSELHFVNSAGLGAFIALHRRCRELGGEVALATPRRAVEQVLRVTNLDR
                     LMPIFPDVESALRQWAQLPAAT"
     gene            complement(1863..2318)
                     /locus_tag="PLU99_14520"
     CDS             complement(1863..2318)
                     /locus_tag="PLU99_14520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOO18306.1"
                     /translation="MTVPPEFGIRESDLTEMVVPSNLRSAKEPERRIVRAMVRNGYDP
                     ETTFGLKLALEEAMTNAVKHGNCNDPSKSVVVRYYVDPRRVVVMVRDEGRGFCPENVP
                     DPTAEENLERPNGRGIMLMHSYTTRVCDNRTGNEVWMLKEKSPPPQPTR"
     gene            complement(2341..2706)
                     /locus_tag="PLU99_14525"
     CDS             complement(2341..2706)
                     /locus_tag="PLU99_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOO18307.1"
                     /translation="MEQPSSHLKIRRSDDVSLVEFADRKILEELSIQEIEEELTRLVE
                     GQTGLKLVLNFKNVDHLSSAALSMLLKLNRRLEEQNGQLKLSDINRQIFEVFKITRLN
                     RIFQIHDTVEDAIAGFGRV"
     gene            complement(2905..3330)
                     /locus_tag="PLU99_14530"
     CDS             complement(2905..3330)
                     /locus_tag="PLU99_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18308.1"
                     /translation="MGRWTYYSPFFIGGILAALLLSTYREFLPTEAPWQFWGLLLLVA
                     CGAGLACQLLMLGAQGAFAQVLPATGGRSVRGSGAMLAGWLLMGGEVLAIVAALLAVE
                     GVRVAAIVFGALALLTLLGAVTTYAWMWPTAVRDFADER"
     gene            3603..>3756
                     /locus_tag="PLU99_14535"
     CDS             3603..>3756
                     /locus_tag="PLU99_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18309.1"
                     /translation="MTGRTNVTQRPHFKHVAIVGATGAVGREFGLVLEQRRFPAQQYR
                     LLASANS"
BASE COUNT          724 a         1295 c         1088 g          649 t
ORIGIN      
        1 agcgattggc cgcgtcgggt atcgccggcg gcgaatacgc cgtccacgct cgtgttcgtg
       61 cggccgggtt gcgtcaggcg aatgtagcct ttctcgtcca gctcgagaca gtcgcgaaac
      121 acctgcgtgt ttggcgtgtg ccctatggcc aggaacatgc ccttgatcgg catctcccac
      181 tctttgcccg tcttgaggtt gcgcaagcgc accccggtga tcatctcgtc gcctagcacg
      241 tcggtgacga cggcatccca gatcggcgcc atcttgcctt cttcgaccag cttgaagaag
      301 cgttcctgca tggccttgct ggcgcgcagc ttgtcgcggc gatgaaccag gtaaaccttc
      361 ttggcgaact tggtgagata gctgccctcc tcgatggccg agtcgccgcc gccgaccacg
      421 atcacctcct gatcgcgata catcggcaac gccccgtcgc acaccgcgca agccgagacg
      481 ccgccgccgc tcttggccag gcgctgctcg ttcggcaggt cgagccagtt ggcccgcgcc
      541 ccggtggcga cgatgaccgt gtgcgcccag gcctcggtct cgtcgccctt gatataaaag
      601 gggcgctcgc ggaagttcac cgccaccacg tcctcagtaa taaagcgcgc cccgaaccgc
      661 gccgcctgct cgcgcatggc cttcatgagc gtggggccga gcacgccctc gggaaagccg
      721 ggatagttct cgacttccgt ggtgaacatg agttgcccgc ccgggatcag cccgcccgcg
      781 gaaaaacctt cgaacaccaa gggctggcag tcagcccgcg ccgcatagat ggcagccgtc
      841 agcccggccg gacccgaacc gataatgacg agtctctcga ctttcatgcg tactcctagc
      901 ctgcgttaac cgcgtcccgc acatgatacg ggaaacgccg cctccctgcc gggcatcgca
      961 cggccggaac tgcaagcgcc cgcgtaaagc gacgtagccc gcccagcacc acccagccat
     1021 tgccgtactg tcgcggtggc cggaacgact ccggcggcgg gcgcagcggg ccgagccaaa
     1081 tcagtgttcc gtagcggcag aaccggcgag ccctccagta tgcttcaggg gcgcaaatcc
     1141 acttgccgtg gcggcggcga atccgatcga gcgcgtctcg gtccaagcga tcgtactcct
     1201 tcacgagcat cgcttccgac atgcccaagg tctgcccacc actcagcttg aacagcagaa
     1261 tgtcaccgcg cactacgcgc ccgaacggcg cccggcgcga tttcgaaaaa cgagattcaa
     1321 ttcgcttctg cccggacaag agccgttgcg ccgtgacccg gtcgactatc gcaaggtgca
     1381 attgcgccgg ccggccgctc gaatatccgt acgcagattg acgcggcatg ctgcaaatcg
     1441 tcccctcaaa acagacagtt tgtcccgcgg ctcgactcag gccgatgggg gttatgtcgc
     1501 ggccggcagc tgcgcccatt gtctgagcgc cgattcgaca tccggaaaaa tcggcatcaa
     1561 ccggtccagg ttcgtgacgc gcagcacctg ttcgaccgcc cgccgcggag tcgccagcgc
     1621 cacctcaccg cctagctcgc gacaacggcg gtgaagcgcg ataaacgcac ccaacccggc
     1681 cgaattcacg aagtgtagct ctgacaggtc tagcacgacc cgcgcattct cggcgcaggg
     1741 cacctccagc agccgccgcc gcagctcgtc aacgtgctcc atgccggccg agccgtacag
     1801 gcgtatgaca taggcgccgc cggcctgttc gactgcggcc cggaaggact cgttgcacgt
     1861 gttcaccgag tcggctgggg agggggcgac ttttccttca gcatccatac ttcgtttccg
     1921 gttctgttgt cgcagacgcg cgtcgtgtaa gaatgcatga gcattatgcc gcggccgttg
     1981 ggccgctcca ggttttcctc cgcggtcggg tcgggcacgt tttcggggca gaatccgcgg
     2041 ccctcgtcgc gcaccataac caccacccgc cgaggatcca cataataacg caccacgacg
     2101 ctcttcgagg gatcattgca gttgccgtgc ttgacggcgt tcgtcatagc ctcttccagc
     2161 gccaacttga ggccgaaagt ggtttcgggg tcatacccat tacgaaccat ggcgcgcacg
     2221 atgcggcgtt cgggctcctt ggcgctgcgc agattactgg ggaccaccat ttcggtcagg
     2281 tccgattcac ggatgccgaa ctctgggggg accgtcatcg ctacctgtcg tcaccgcgct
     2341 ctaaacgcgg ccgaaaccgg caatcgcatc ctcgactgtg tcgtggatct ggaaaatcct
     2401 gttgagcctt gtgatcttaa agacttcaaa aatttgacga ttgatgtcgg agagcttcaa
     2461 ctgcccgttc tgttcctcga ggcggcggtt gagcttgagc agcatgctca gggcggcgct
     2521 ggacaggtgg tccacgttct tgaagttcag caccagcttg aggcccgttt ggccctctac
     2581 cagccgcgtc agctcttcct cgatctcctg aattgaaagc tcctcgagga tcttgcgatc
     2641 ggcgaactcg accagcgaaa cgtcatcgga gcgcctgatt ttcaggtgcg aggacggttg
     2701 ttccatagtg cagctccact cccgcgggcg cacaccaacc tccagccggt tgaaggctcg
     2761 ccgtcgcccg aattgttatg attctctatc ccactcagtt gtatgtcaac tcggcggcat
     2821 accagtcggc aagatatcgg accatacggt acaaacatct atcagcccta gcagcgacgc
     2881 ccggccatcg tttcaagacc aggttcagcg ctcgtccgcg aaatcgcgca ccgccgtcgg
     2941 ccacatccag gcgtacgtcg tcaccgcgcc cagcaacgtc agcagggcca gcgcgccaaa
     3001 aacgatcgcc gccacgcgaa caccttcgac cgccagcagg gccgccacga tggccagcac
     3061 ctcgccaccc atcagcagcc agccggccag catggccccg ctgccccgca ccgaccgacc
     3121 gcccgtcgcc ggcagcacct gcgcgaacgc cccctgcgca cccagcatca gaagctggca
     3181 agccagcccg gctccacagg ccaccagaag cagcagcccc caaaactgcc agggcgcctc
     3241 cgtcggcagg aattcgcggt aggtcgacaa cagcagcgcg gccaggatgc cgccgatgaa
     3301 aaaaggcgag tagtacgtcc agcgtcccat gcatgatctc cggcctgccg atgcgccacg
     3361 aggcggtagc tctcaagagt acgccacacg tacgccgcag ttcctggagc ggttcgccag
     3421 cggcgaactg agttcgccga cggccaaccg ggctcgcccg tcggcgaact tgcgtgaggc
     3481 gacgcaggcc aacaatacat gttaccatag acgatccgag ccccaagcgc cagcaagggc
     3541 aataatccga acgggcaacg acccgagccc gaagcgctag cgagggcaag tcaaggacga
     3601 taatgaccgg tcgaacaaac gtcacacaac ggccccattt caaacacgtc gcgatcgtcg
     3661 gcgccaccgg cgcggtcgga cgcgaattcg gtctggtcct cgagcagcgc cgcttcccgg
     3721 cgcaacagta ccgcttgctg gcctcggcaa actcgg
//
LOCUS       DAOSYS010000316         3737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19809_length_3737_cov_5.855785, whole genome shotgun sequence.
ACCESSION   DAOSYS010000316 DAOSYS010000000
VERSION     DAOSYS010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19809_length_3737_cov_5.855785"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1164
                     /locus_tag="PLU99_14540"
     CDS             <1..>1164
                     /locus_tag="PLU99_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18310.1"
                     /translation="QPDVPALLVDVTRLAEAGRTTDAADALANLLEMQPPLPVEDQIE
                     LHDRLAELLFEQEQRRPVALAANLERLLEHVEAAERLGAPRTARRILRSAQVQEWLGR
                     PEDAIRSCREALQTDPSPEEGRAAMRSLVRLLDGRPEAAAQREELLETLLSDESLDDD
                     SAWWAMRQAVDAALEKDDPLQARLLLSKYAARLSTSDLKGYFEYLWACVLMHEGRFEE
                     AAPLVYWVDEWIKRIGQEVFNSERLRELTGLNRWLLGRVYLAQEQPDEALAAFEEAAA
                     FKPDDPAAAGAATVGQAEALAGLGRHEDSRAALRAAAQMLPTSPGERDRALKRVRATV
                     RELWEARLEAADYANAQDYLSLAAELTPPDLPAEKLEILEKLGGTALLAANAA"
     assembly_gap    1165..1264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1265..2102
                     /locus_tag="PLU99_14545"
     CDS             <1265..2102
                     /locus_tag="PLU99_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18311.1"
                     /translation="GNAEEALRWYARLREQYPALEEAFRGALRSAGCLIEAEREAEAE
                     ALLLRLLDNDVITPQAQAFRGGLLQLCELLYQQERYGEAIGRLEDYLARYPTDAQRGR
                     ARFLRADAYRRSACQLRDAPVGAGEAQQYRALALERFRIAAELFAELETDFAAAADPD
                     DETELYQRLALLYSADCLLELNDAPALEQALQYCRRAASAFEQEPPALTAQIQIANIH
                     LRRGQPADAARAVERARWLLRSMPNQAFDHWPGPGRADWEAYLAAISSARLFESEFAG
                     RP"
     gene            2126..2590
                     /locus_tag="PLU99_14550"
     CDS             2126..2590
                     /locus_tag="PLU99_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18312.1"
                     /translation="MYAHESQEPKRLITLLSEQRELYQRLRDLSERQRSLISSDRPEQ
                     LLSILRERQTLVAALAKLNEQLAPFRRNWDILYQGLPADLRVSANKLLQDINGLLRVI
                     LKTDQEDSALLAARKQSVGQRLRDVGGGQTANTAYARQSGAPSLAPGADMTG"
     gene            2638..3495
                     /locus_tag="PLU99_14555"
     CDS             2638..3495
                     /locus_tag="PLU99_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOO18313.1"
                     /translation="MSASPSELEQVLHDYMDVTRRLQQTHEALQREVVRLRDELAAKN
                     RELEVGRRLAALGELAAGLAHEVRNPLGAIQLYSGLLKQKCAQLEPALGLIEKMELGI
                     QAIDAVVRDALALAPRCRPGCVHLLSETIAATQNNCRQKLQEHQVRLVVRMPKRAVYV
                     RAEPDGLQRVLLNLVCNAAEAANPGGRIDLRVAAGRQGKVEIRVRDDGPGLSDEALER
                     MFDPFFTTKQNGTGLGLTIARRLVESYGGTLSARNHAAGGAEFSIVLPTADQEAEHGP
                     SAAPQTSAA"
     gene            3514..>3737
                     /locus_tag="PLU99_14560"
     CDS             3514..>3737
                     /locus_tag="PLU99_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO18314.1"
                     /translation="MACICVIDDQALIRDSLSATLAAQDHKVFTFDNAQDALTELRQR
                     AFDVVLTDLRMPGMDGVGLRRSVSTTSKAR"
BASE COUNT          656 a         1132 c         1280 g          569 t
ORIGIN      
        1 tccagccgga tgtgccggcg ctgctggtgg acgtgacgcg gctggcggaa gcgggacgaa
       61 ccacggacgc ggcggatgcc ttggccaacc tgctcgaaat gcagccgccg ttgccggtcg
      121 aggatcaaat cgagttacac gaccgcctgg cggagctgct ttttgaacag gagcagcggc
      181 gcccggtggc tctggctgcc aatctcgagc gactgctgga acacgtcgag gctgcggagc
      241 ggctgggcgc gccgcgcacc gcgcggcgca ttctacgtag cgcccaggtg caggagtggc
      301 tgggtcggcc ggaagacgcc atcaggagtt gccgggaggc gctgcagacc gatccgtccc
      361 ccgaggaagg tcgtgccgcg atgcgcagcc tggtgcgcct gctggacggc cggccggaag
      421 cggcggcgca gcgcgaagag ctgctcgaaa cgttattgag cgacgagtcg ttggacgacg
      481 actcggcctg gtgggcaatg cggcaggcgg tagatgctgc gctcgaaaag gacgacccgc
      541 tgcaggcgcg tctactgctg agcaaatatg cggcgcgtct gtcgacgtca gacctgaagg
      601 gctacttcga atacttatgg gcttgcgtgc tgatgcacga ggggcgcttc gaggaagccg
      661 cgccgctcgt gtattgggtt gatgaatgga tcaagcgaat aggccaggaa gtcttcaact
      721 ccgagaggtt gcgcgaactg actggtctga atcgctggct gctggggcgg gtctacctgg
      781 cgcaggagca gcccgacgag gccctggcgg cgtttgaaga ggccgcggct ttcaaaccgg
      841 atgatccggc cgccgccggc gcggcgaccg tcggccaggc tgaagcgctg gctggcctgg
      901 gacggcacga agactcgcgc gccgccttgc gcgcggccgc gcagatgctg ccgacaagcc
      961 ccggcgagcg cgatcgcgcg ctgaaacggg tgcgcgcgac ggttcgagaa ctctgggagg
     1021 cccggcttga ggccgcggac tacgccaacg cacaagacta cctctcgcta gcggccgaat
     1081 tgactccgcc tgatttgccg gccgagaagc tggaaatcct ggaaaagctg gggggaacgg
     1141 cgttgttggc ggcgaacgcg gctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnngggcaa cgctgaggaa gccctgcgtt ggtacgcccg gctgcgggag cagtacccgg
     1321 cgctggagga ggccttccgc ggcgcgctgc gcagtgcggg ctgcctgatc gaagcggagc
     1381 gcgaagccga ggccgaagcc ctgctgctac ggctgctgga taatgacgtg atcacgccgc
     1441 aggcgcaagc ttttcgtggc ggcctgctgc aactgtgcga gttgttgtat cagcaggagc
     1501 gctacggtga agccattggc cgcctggaag actacctcgc ccgctatcca accgacgccc
     1561 agcgcgggcg ggcgcggttc ctccgcgcgg acgcgtatag acgcagcgct tgccaattgc
     1621 gcgacgcccc agtcggcgcg ggcgaagcgc agcagtaccg ggcgctggcc ctggaacgtt
     1681 tccgcattgc ggcggagctc ttcgccgagt tggagacgga ttttgcggcg gcggccgatc
     1741 ctgacgacga gaccgagctt taccagcgtc tggcactgct atacagcgcc gactgtctac
     1801 tggagctgaa cgacgcgccc gcactcgagc aggcgctgca atactgccgc cgcgccgcgt
     1861 ccgctttcga gcaggagccg ccggctctaa cggcgcaaat ccaaatcgcc aatatccacc
     1921 tgcggcgcgg gcaaccggcg gatgcggcgc gagccgtcga acgggcacgc tggctcctgc
     1981 gcagcatgcc gaaccaggcc tttgaccact ggcccggccc gggtcgtgcc gactgggaag
     2041 cctacttggc cgcgatttct tcggcgcggc tgtttgaaag cgagtttgcc ggccgcccct
     2101 gaggcaggct tttgaaggag ttgtcatgta cgcgcacgag tcgcaggaac ccaaacggct
     2161 gatcacgctg ttgagtgaac agcgcgagct gtaccagcgt ttgcgggacc tgagcgagcg
     2221 gcaacgctcg ctgatctcca gcgaccgccc cgagcagttg ctgagtatcc tccgcgagcg
     2281 gcagaccctg gtggccgcgc tcgccaagct caacgaacag ctcgcaccct tccgccgcaa
     2341 ctgggacata ctgtaccagg gattgccggc ggacttgcgc gtgagcgcca acaagctgct
     2401 gcaagacatc aatggcttgc tgcgtgtcat tctcaaaacc gaccaggaag acagcgccct
     2461 gttggcggcg cggaaacagt cggtcgggca gcggctgcgc gacgtcggcg gcgggcagac
     2521 ggccaatacc gcttatgccc gccagagcgg cgcgccctcg ctggcccccg gcgcggacat
     2581 gaccggctga gtacacgagg cgcagcacag atgatgcccg gcaaggaagc cgcaaacatg
     2641 agcgcgagcc ccagcgagct ggaacaagtg ttgcacgatt acatggacgt gacgcgccgt
     2701 ctgcagcaaa cccacgaagc gctgcagcgt gaggtggtgc gcttgcgcga cgaactggcc
     2761 gccaagaacc gcgagctgga ggtgggccgc cgcttggccg ccctgggcga attggcggcc
     2821 ggcttggccc acgaagtgcg caacccgttg ggcgccattc agctctacag tggactgctg
     2881 aagcagaaat gcgctcagct cgaacccgct ttgggtttga tcgagaagat ggaactgggc
     2941 atccaggcga ttgacgcggt ggtacgcgac gccctggcgc tggcgcctcg ctgccggccg
     3001 gggtgcgttc acctgctgag cgaaaccatc gcggccacgc agaataactg ccgccagaag
     3061 ctgcaggagc accaggtgcg gcttgtggta cgcatgccga aacgcgcggt ttacgtgcgg
     3121 gccgagccgg acgggctgca acgcgtgctg ctcaacctgg tgtgcaatgc ggccgaggcc
     3181 gccaatcccg gcgggcgcat cgatctgcgg gttgcggcag gccgtcaggg caaggttgaa
     3241 attcgcgtgc gcgatgacgg cccggggctg tctgacgagg ccctggaacg catgttcgac
     3301 ccattcttca ctactaagca aaacggcacg gggctggggc tgaccatcgc tcgccggttg
     3361 gtagagtcct acggcggcac cctcagcgcc cgtaaccatg ccgcgggcgg ggcggagttt
     3421 tccatcgtcc tgccgaccgc ggaccaggaa gctgaacacg ggcccagtgc cgcgccgcaa
     3481 acgagcgcgg cctgaggcaa ggagagagga taaatggcgt gcatttgtgt catcgatgac
     3541 caggctttga tcagggactc gctgagcgcc acactggccg cacaggacca caaggtgttc
     3601 acgttcgata atgcgcaaga cgccttaact gagctgcgcc agcgggcctt tgacgtggtg
     3661 ctgacggacc tgcgcatgcc cggcatggac ggggtgggct tgcgcaggtc cgtcagcacc
     3721 acgtcaaagg cccgctg
//
LOCUS       DAOSYS010000317         3715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_19953_length_3715_cov_5.914754, whole genome shotgun sequence.
ACCESSION   DAOSYS010000317 DAOSYS010000000
VERSION     DAOSYS010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19953_length_3715_cov_5.914754"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLU99_14565"
     CDS             complement(<1..588)
                     /locus_tag="PLU99_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18315.1"
                     /translation="MTEMPAAVGGDPAVATVEYYDASSSTTGASKGQIALVVDANGVA
                     HGYKYDKYGYLSQELEGLAQLSDRLSILPPFVHPATPVGGASPNTNNGIGQVTCFRYA
                     GCSCCVLYNANGQQITCPIWTCPIDDDCGRLGSVASPCFRARQDCFSDPEYDVLGRLH
                     SITRCAELGTSSMAAEYDELGRLSVLTKSAGEDYDT"
     gene            772..2508
                     /locus_tag="PLU99_14570"
     CDS             772..2508
                     /locus_tag="PLU99_14570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOO18316.1"
                     /translation="MASVTEAINDPDMPSRQNWMPTGMTGLDSRGNWRDFASTIAGQT
                     ATQARSLDEAGGGGGCTNEYERIDMTLGTQHTIQQLTYDEAGNLVAADLLGDMNCDGL
                     VKSFDIDPFTQALTNPTAYAAAHPDCMIELGDINGDGLVNAFDLNAFVDVLGDNLMPA
                     RRYEYDEENRLTAVTDIHGDPLLEVEYDALGRRIASVDYEAPGGPVQTRHIYSGLNTV
                     AEYIWSGSQWTLAREFLWGERFPEPLVLIDFTDAGEIGAGSAEVLHYVHDALGSVVGL
                     VDAGNAPTVQPKLVERYDYDPYGKTYIETWDAEAGGGSGAWVRTTVSAYGNPFGWTGQ
                     RYDASVGLYHFQFRSYSPEMGRWIQRDPLRYFGGINLYCYVLSAPVDLIDSLGLYPGQ
                     GIVRKVSEAVGGVLAVGPLDAWDGGAGESYKKSCQYSEKYKDQGSAADPGGAQNAARH
                     ADWQARLAYKHDEDTAKEIGDIHEAGNPDNPDTQADKHNNSVGREIGKKAKEEGRPID
                     DIDAMVDEAMADGRLIRDRENDERITTPGEPPANPRSDSPTSPDDSSPDSNKSESPSN
                     EPSKPPKASACS"
     gene            2541..2912
                     /locus_tag="PLU99_14575"
     CDS             2541..2912
                     /locus_tag="PLU99_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18317.1"
                     /translation="MIHVSRRLCIAGLLALLALSALVVIRLGGCESWSPSSIEESKKR
                     AEPIIAALEAYYTAHGQFPKTLQDLVPEHIDELPQPAAGTGQWHYASREGSFSLSFGC
                     GKHHYPNCTFSWKSRNWLLDH"
     gene            complement(3062..3331)
                     /locus_tag="PLU99_14580"
     CDS             complement(3062..3331)
                     /locus_tag="PLU99_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18318.1"
                     /translation="MPRTLVIDNLKAGVIKADWFDPVLNPKLQAFAEHYGTVIMPTKP
                     YTPRHKGKVERGVDYVQENALKGRTFDSLTAPERLPGRLGDQRRR"
BASE COUNT          707 a         1097 c         1237 g          674 t
ORIGIN      
        1 cgtatcgtag tcctcgccgg cgctcttggt caggaccgat agacgcccga gttcgtcgta
       61 ctccgcggcc atggaagacg ttcccagttc ggcgcaacgc gttatgctgt gcaggcgccc
      121 caacacatcg tactcgggat cggagaagca gtcctgcctc gctcggaagc acggcgacgc
      181 cacgcttcca agccgcccgc agtcgtcgtc tattgggcac gtccagatcg ggcacgtgat
      241 ctgctgcccg ttggcgttgt acagcacgca gcacgagcaa ccagcgtagc ggaaacacgt
      301 cacctgcccg attccgttgt ttgtattcgg gctggccccg cccaccggcg ttgccgggtg
      361 aacaaaaggc ggcaggatcg acagtctgtc ggagagctgc gccagcccct ccaactcctg
      421 ggacaggtag ccgtatttgt catacttgta gccgtgggcg acgccgttgg cgtccaccac
      481 cagcgcgatc tgccctttcg atgcgccggt ggtcgagctg ctagcgtcgt agtattccac
      541 cgtagcgacg gccgggtcgc cgcccacggc ggcgggcatc tccgtcacgc tggtaaccag
      601 cgtcggccac agaggcaaat cgtacgcgta ctgcgtccaa tgatattccg gatccgtaac
      661 ttgcgtcagg cggtacaggt ttggccaagt ttgggcgtcc ggctgctccc aggtgtacgt
      721 ccacgtctgg tccgtaatcg ggctggttag cgtctcgagg ttgccggcgg cttggcaagc
      781 gtcactgagg cgatcaacga cccggatatg ccctcgcgcc agaactggat gcctaccggg
      841 atgacgggcc tcgattcgcg cgggaactgg cgcgattttg cgagtacaat cgccgggcag
      901 acagcgacgc aggcccgcag cctggacgag gccggcggtg gcggcggctg tacgaacgag
      961 tatgagcgga tcgacatgac gctcggcacg cagcacacaa tccagcagct aacgtatgac
     1021 gaggccggca atctcgtcgc ggcggacctg ctgggcgaca tgaactgcga tgggctggtg
     1081 aagagcttcg acatcgaccc gttcacgcag gcgctgacca acccgaccgc gtacgcggcg
     1141 gcccacccgg attgcatgat cgagctgggc gacatcaacg gcgacgggct ggtcaacgcg
     1201 tttgacctaa acgccttcgt cgacgtgctg ggcgacaatc tcatgcccgc ccggcggtat
     1261 gagtatgacg aggagaaccg cctcacggcc gtgaccgaca ttcacggcga cccgctgctg
     1321 gaggtggagt acgacgcgct ggggcggcgg atcgcgagcg tcgattacga agcgcccggc
     1381 gggcccgttc agacgcggca catttactct gggctgaaca cggtggcgga gtacatctgg
     1441 tccggctcgc agtggacgct ggcgcgcgag ttcctgtggg gtgagcgctt ccccgagccg
     1501 ctggtgctga tcgacttcac ggatgcgggt gagattgggg caggctcggc cgaagtcctg
     1561 cactacgtgc acgatgcgct gggcagcgtg gtcgggctgg tggatgccgg caatgcgccg
     1621 acagtgcagc cgaaactggt ggagcgctac gactacgatc cgtacggcaa gacgtacatt
     1681 gagacgtggg acgccgaggc cgggggaggg agcggagcct gggtgcgtac aactgtgtca
     1741 gcttacggca acccgttcgg ctggacgggc caacgctacg acgcgagtgt cgggctgtac
     1801 cacttccagt tccgcagcta ttcgccggag atgggaagat ggatacagcg ggatccgctg
     1861 cgttacttcg gtggtatcaa tctctattgt tacgttttgt ccgcgccggt ggatttgatc
     1921 gacagccttg gcctgtatcc tggccaaggc atcgtgcgaa aggtcagtga agctgtcggc
     1981 ggcgttcttg cggtggggcc tcttgacgca tgggacggcg gcgctgggga gtcatacaag
     2041 aaatcgtgcc agtattccga aaaatacaag gaccagggct ccgctgccga tccgggcgga
     2101 gcgcagaatg ccgcgcgcca cgcggactgg caggcacgcc tagcctacaa acatgacgag
     2161 gatacagcca aggagatagg cgacattcac gaagcgggaa atccggataa tccagatacc
     2221 caggccgaca agcacaacaa ctctgttggg cgggagatcg ggaaaaaggc gaaagaggag
     2281 ggtcgaccta tcgatgacat tgacgctatg gttgacgaag caatggcgga cggtcgtcta
     2341 atccgcgacc gtgagaacga cgaaaggatc acgacgccgg gtgaaccgcc ggcgaatcct
     2401 cgaagcgatt cgccaacttc ccccgacgac tcgagccccg attccaacaa atcggagtcc
     2461 ccaagcaatg agccgagcaa acccccgaaa gccagcgcgt gtagttagcg tctaaggcgg
     2521 cgcaggttgg aggacctagg gtgattcacg taagcagacg cctctgcatc gccggactgc
     2581 tcgcgctgct cgcgttgtcc gcactggtcg tcatcaggct gggcggctgt gaatcctggt
     2641 cgccgagcag cattgaggaa agcaagaagc gcgcagaacc tatcatcgcc gcgctcgagg
     2701 cttactacac ggcccacggc cagtttccga aaacccttca ggacctcgtg cctgagcaca
     2761 ttgacgagct tccgcaaccc gcggcgggga ccgggcaatg gcactacgcg tcgcgggagg
     2821 gatcatttag cctttcattc ggttgcggta agcatcacta cccgaattgc accttttcct
     2881 ggaagagcag gaattggttg ctggaccact aatctgccga tgaggagaag tagtgcatac
     2941 ggactgaagt gagcagggta gttccgtgga tggaattcgt ttacggaacc gcaacggcac
     3001 gcgctcgctc agggagtttg acgggctcta tcgcctgcaa ggacacggag acgctgttcg
     3061 gctatcggcg acgctggtct cccagtcggc caggaagtcg ttctggggcc gtcaggctgt
     3121 cgaaggtgcg tcccttgagg gcattctcct gaacgtagtc cacgccgcgt tcgaccttgc
     3181 ctttgtgccg cggcgtgtag ggcttggtgg gcatgatcac cgtgccgtag tgctcggcga
     3241 aggcctgcag cttgggattg agcacggggt cgaaccagtc ggccttgatc acgccggcct
     3301 tgaggttgtc gatgacgagc gtccgcggca cgccgccgaa gtgccagagg gcgttctcca
     3361 ggcagcggat gaagttcgcg aatcgtctga cgctcgacgg cctcgctgta ggccttgcgc
     3421 gagcagctca ggacgatgcg gaagacgtgc gggcacttgc gtttgccgtc tggcgtgatg
     3481 atcggggccc cgcggccgaa atccacctgg gcttcctggc ccggggcgga ctccatccgg
     3541 cggaacggca agggcgtggt gcggcgtagt gtccgcacga agcgctgcac ggactggtag
     3601 gagccgtcga agccgtgctc ggtcttgagg tcctgccaga tacgctgggc cgtcagtccc
     3661 agctccagct tggtctggat cagttcgcgc aagggctcac agcggttgcg tcgtc
//
LOCUS       DAOSYS010000318         3659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20340_length_3659_cov_4.866537, whole genome shotgun sequence.
ACCESSION   DAOSYS010000318 DAOSYS010000000
VERSION     DAOSYS010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20340_length_3659_cov_4.866537"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..2242
                     /locus_tag="PLU99_14585"
     CDS             506..2242
                     /locus_tag="PLU99_14585"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18319.1"
                     /translation="MNEVDKQPKPAGRGAIAVALPNLLVLTRCRAVQTRNLIDQQLKN
                     APIRTFVVIVLLAAIWAALYFLLNAIFHQINRWDLVAVVASKPIFVNFFLVLAIMLAF
                     SNAILGFGSLFGRGDCAYLLAMPIHPRQVLTVKWLEGMLLSGWSFLLLGVPLMLAVAN
                     NATVPWYYFPLFIGHFVGFMLVPATLGLLLAWAVAMWVPRQPLMLIVWIIVIAGLAGG
                     LYLWNFSHSEVDSNEWLAMLSAEVSIAKAKLLPSTWTAEGVVAAIEKRPADSLHYLLV
                     VLGNAAFLCWLTINLIARTWPEAYSRARYGFRAGTTRGGWVTAAVCRVLFFYLPRQLR
                     VIMLKDVRAFDRDATQWTQTAIMLGLLVIYALNLRRLPLDLSQPVVKAVMAFLNLAII
                     SLIIATFTSRFVFPLLSLETRQMWLLGMLPAGRSAVLTVKFVFAVTVTSLAGLGVMSL
                     AVRMLDLPDDWASFSLFVCFLICSGLSGLSVGLGARFPVLGQRNPVRIASGLGGTVNL
                     VASMVFVGIALLIVALAGWRQIAHAGLTGGPLVPLTQQTWQAAGLIVLVSMLVTIAPL
                     WAGSRHFQRLEC"
     gene            2477..>3659
                     /locus_tag="PLU99_14590"
     CDS             2477..>3659
                     /locus_tag="PLU99_14590"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOO18320.1"
                     /translation="MTKCFNQTGCPAPGTGGPAGPERFLRTDFLQSDLKARSVRGGAV
                     TLSAQGARFVLQTVSTLLLARLLAPAEFGLVAMVIVAINFVALFKDFGLSFATIQREQ
                     ISQAQISTLFWINVFIGVCLSALAAAAAPFVARFYAEPRLVPLMLALSPTLLVSGLLA
                     QHQALLTRQMRFAAIAIADVGAMVVGITVGISLALGGARYWALVGMMLSQNAVNLLLV
                     WWLCPWRPSRPTRGAGVRSLLTFGGNVTAGALLNYIGSMADRVIIGRLWGPLPLGLYD
                     RSHALVLMPMLQINPPLAAVALPALSRAAATPERYARAYVSILRQLALVTAVLVPILI
                     VNASTVIRVVLGPSWAAAGPLFGVLGFAALLLPVWNAIGWVFISQNRTRELLNWHALD
                     LV"
BASE COUNT          536 a         1256 c         1077 g          790 t
ORIGIN      
        1 cggcgggcct caggttatca aaatcgtgtg cacaaaaacc atgagtatct gatcatctgc
       61 ccccaagacg cgaccccaca cgacttttat cagaccgctt gagcacctgg ggcagcaccg
      121 ccgcgcgccg ccgctcgcgc gcgaattcgt ctcgcacgcg ctctgtcggc aatcccactt
      181 atccgctttc tcctcgtggc cgctgcccgt tttggcgccg gcgttatcgg acacgccccg
      241 cagccgttgc cggatcggat tgccgacgtt tgccctcgcc ggcgcttcgg actcggattg
      301 ccgacgtttg ccctcgctgg cgcttcgggc tcggattacc ccctgcccgc ctgccgctct
      361 cccgcccgcc gccccaccat tgccggccgc ttccacgccc ggactgccgt caccctgtac
      421 aatgctttcg ataacgagga acccgtgaac aagcgtcgat aagcaacgct tctgatgagg
      481 aacccgtgaa gagggtcgat aaagcgtgaa cgaggtcgac aaacagccaa aaccggccgg
      541 gcgcggcgcg atcgccgtgg ctctcccgaa cctgctggtt ctcacgcgct gccgcgcggt
      601 gcagacgcgc aacctgatcg accagcaact gaagaacgcc cccatcagaa ccttcgtcgt
      661 aatcgtcctc ctcgccgcga tctgggccgc cttgtacttc ctgctcaacg cgatcttcca
      721 ccagatcaac cgctgggacc tcgtcgccgt cgtcgccagc aaacccatct tcgtgaactt
      781 cttcctcgtc ctggccatca tgctggcctt ctcgaacgcc atcctcggct tcggcagcct
      841 tttcggccgc ggcgattgcg cttatctgct ggccatgccc attcatccgc gacaggtact
      901 caccgtcaaa tggctggagg gaatgctgct ctccggctgg tcgttcctac tcttgggcgt
      961 gccgctcatg ctggcggtcg ccaacaacgc cacggtgccg tggtactact tcccgctgtt
     1021 catcggccat ttcgtaggct tcatgctcgt gccggccact ttggggctgc tgctggcctg
     1081 ggccgtcgcc atgtgggtgc cgcgccagcc gctaatgctc attgtgtgga tcatcgtcat
     1141 tgccgggctg gccggcggcc tctacctatg gaacttctcc cacagcgagg tggactccaa
     1201 cgaatggctg gcaatgctgt ccgcggaagt cagcatcgcc aaggccaagc tgctgcccag
     1261 cacctggacc gctgaaggcg tcgtggccgc gattgaaaaa cgccccgccg atagcttgca
     1321 ctacctcctg gtcgtcttgg gcaacgccgc cttcctgtgc tggctgacca tcaacctgat
     1381 cgcacgcacc tggcccgaag cgtacagccg cgcacgatac ggcttccggg cgggcaccac
     1441 gcgcggcggc tgggtgaccg cggcggtgtg ccgcgtgctg ttcttttacc tgccgcgcca
     1501 attgcgcgtg atcatgctca aggacgtgcg cgcctttgac cgcgacgcca cgcaatggac
     1561 gcagaccgcg atcatgctcg gtctactcgt catttacgcg ctgaatctca gacgcctccc
     1621 gcttgatctg agccagccgg tcgtcaaagc cgtcatggcc tttctcaact tggcgatcat
     1681 cagcctcatc atcgcgacat tcaccagccg tttcgtcttc ccgctgctct cgctcgaaac
     1741 acgccagatg tggctgttgg gaatgctgcc ggccgggcgt tccgcggttc taacggtcaa
     1801 attcgttttc gcggtgacgg ttacgagtct ggcgggcctg ggcgtcatga gtctggccgt
     1861 gcgcatgctc gatctgcccg acgattgggc tagctttagc ctgtttgtct gcttcctgat
     1921 ttgcagcggc ctgtcgggcc tgtcagtcgg cttgggggcg cgctttcccg tgctcggcca
     1981 gcgtaacccg gttcgcatcg cctcgggtct cggcggcacg gtcaacctcg ttgccagcat
     2041 ggtgttcgtg ggaatcgctc tactcatagt tgcgctggcc ggctggcggc aaatcgccca
     2101 cgcgggtctg acgggcgggc cgctcgtgcc gttgactcaa cagacctggc aagcggccgg
     2161 cctgatcgtg ctcgtcagca tgctggtgac gattgcgccg ttgtgggccg gctcgcggca
     2221 tttccaacgc ctcgagtgct gaactgcccg tcgagcttac gatcctgtca cgcctttgga
     2281 cgcgctgcaa caacatcgta atctttgcaa ttgagttctc gccgggtttg cgccccacct
     2341 atactactgc gttgaggtgt tcgaagctct ttacctggat tgaattgcgc atgcaccgcg
     2401 cgccggacac acgcccgtct cagcccgggg gccgaggcgg tgccgcgttg cgcgccgcgc
     2461 gtcccgcgag caggccgtga cgaagtgctt caatcaaacc ggctgccccg caccgggcac
     2521 tggcgggccg gcgggcccgg agcgctttct gcgcaccgat tttctccaaa gcgacttgaa
     2581 ggcccgttca gttcgcggcg gagccgtcac actgtctgca cagggcgcca ggtttgtact
     2641 ccagactgtt tcgacacttc tgctggcacg cttactcgcc ccggccgagt tcggcctggt
     2701 tgcgatggtg attgtggcca tcaactttgt ggcgctcttc aaggatttcg ggctctcatt
     2761 cgccaccatt cagcgcgagc aaataagcca ggcgcaaatc agcacactat tctggattaa
     2821 cgtcttcatc ggcgtgtgtt tgtctgcgct cgccgccgcg gccgcgccgt ttgtagcccg
     2881 tttctacgct gagccgcgcc tggttccgct gatgctggcc ctgtctccca cgttgctggt
     2941 cagcggcctg ctggcgcagc accaggcgct gttgacgcgt cagatgcgtt ttgccgcgat
     3001 tgccattgcc gacgtcgggg cgatggtcgt aggcattacc gtcggaatct cgttggcact
     3061 cggcggcgcc cgttactggg ccctcgtcgg catgatgctc tcgcagaatg ccgtcaatct
     3121 cctgctggtc tggtggcttt gcccgtggcg cccgagtcgc cccacgcgtg gcgccggcgt
     3181 gcgctcgctg ctgacttttg gcggcaacgt cacggccgga gccttgctca actacatcgg
     3241 ctccatggcc gaccgcgtga tcattggacg cctctggggc cccctgccgc tggggcttta
     3301 tgaccgctcg cacgccctcg tgctcatgcc gatgctgcag atcaacccgc cgctggccgc
     3361 cgtggcactt ccagcgctca gccgggccgc cgcgactccc gagcgctacg cccgcgctta
     3421 cgtttccatc ctgcggcagc ttgcactcgt aacggccgtc ctggtcccga tcctgatcgt
     3481 gaacgcctca acggtaatcc gagtcgtgct cggaccgtcg tgggccgcgg ccggaccgct
     3541 attcggcgta ctcgggtttg ccgcgctcct gctcccggtc tggaacgcga tcggctgggt
     3601 gttcatctcg cagaaccgga cgcgtgaact gctgaattgg catgcgctcg acctggttt
//
LOCUS       DAOSYS010000319         3655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20378_length_3655_cov_3.507778, whole genome shotgun sequence.
ACCESSION   DAOSYS010000319 DAOSYS010000000
VERSION     DAOSYS010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20378_length_3655_cov_3.507778"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLU99_14595"
     CDS             <1..1220
                     /locus_tag="PLU99_14595"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOO18321.1"
                     /translation="SPPYDVLDQADKDRFLAADPKNFVAIDLPHTPPKSAGPPEVYEN
                     SRRTMSWWLAEGTLTRDELPMIYVYHQKFRYGDTAYTRKMFFARLKLEPFGTGTVFPH
                     EHTFGGPKEDRLCLTKACSANLSPIFGLYEDARNEVSRLLESKLPAEPLATGKLDGVE
                     NYMWGISAREVIDEAVRLMAPRPIYIADGHHRYGTGMLHRDWLIGERGELPPEHPANF
                     VLCVFCAMEDPGLLILPTHRVLSGVVVKAASLQSDPQLEVRQLNVSGAESVITALRNS
                     GPQAVAFHNPAENGYFVVRPKNPDLLDKLEPDHSPAWRRLGLAFLHAYLLNKVITPQL
                     CGGSEPAVQYIKSADEAVAAADQSQGTVFLLQPNTMEELRNVCSAGDLMPHKSTYFYP
                     KLASGLVINPLDE"
     gene            complement(1353..1478)
                     /locus_tag="PLU99_14600"
     CDS             complement(1353..1478)
                     /locus_tag="PLU99_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18322.1"
                     /translation="MILAGSGLTAGGWVFMLSSLGVVVAVVVFFYSRLLRKPMDK"
     gene            complement(1475..3091)
                     /locus_tag="PLU99_14605"
     CDS             complement(1475..3091)
                     /locus_tag="PLU99_14605"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOO18323.1"
                     /translation="MARPTEQWGTRIGVIMAVAGSAVGLGNFLRFPATAAANGGGVFM
                     IPYFCALLLLGIPICWAEWTMGKAGGRLGLHSCSAILGHLGGHRAWRYAGALGLLMPT
                     VVYLYYVVIESWCLGYAWSYLCGTIDLGSDPAEYAKRSAEVFSGFAGDNFDGVMFNGT
                     VHTSVYFWLIVVAANFWLIYRGISKGIEKFCNLAMPIMAVCAIFVLIRVLTLPAVHEA
                     GETRTVLDGLGFMWNPKPVHEGQSALQALADPKVWLAAAGQVFFTLSVGFGVIVTYAS
                     YIRPKEDIVLTSTTAISTNEFFEVCLGGLITLPAAFIFLGVVGASKGTFGLAFNTLPI
                     VFQNMPGGRLFGFLWFFMLFLAAITSSVSLLQPVIAFLEEALNVTRRTSVTILAVVTL
                     LGNLFVIWFSKNLAALSTLDFWSAEVGILVLGTLEVIVLAWVFGARRGYQVALDGAAM
                     RPPAWMYKYVMRFVTPALLLIVLGMWCYFYMGGHLRSIGQGGVPALAVGLIGLLLAFY
                     LFMAHLADRRWGPNRVAIEVCADVAAEGKK"
     gene            complement(3434..>3655)
                     /locus_tag="PLU99_14610"
     CDS             complement(3434..>3655)
                     /locus_tag="PLU99_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HOO18324.1"
                     /translation="LGIVDYSADLNGDGYVDAADLAIFTTCMAGPDVTTPPPGATPEQ
                     FARADFDGDLDVDLSDLAVFQVAFSGSEN"
BASE COUNT          758 a         1252 c         1062 g          583 t
ORIGIN      
        1 tctcgccgcc atacgacgtg cttgatcagg ccgacaaaga ccgcttcctc gcggctgatc
       61 cgaaaaattt cgtcgcgatt gatctgccac acacgccccc caagtcggcc ggcccgccgg
      121 aagtgtacga aaactcgcgc cgcaccatga gttggtggct ggccgagggc acgctgaccc
      181 gcgacgagct tccgatgatt tacgtctatc accagaaatt ccgctacggt gatacggcct
      241 atacgcgcaa gatgttcttc gcgcgcctca agctcgaacc cttcggcact ggcacggtgt
      301 tcccccacga acacaccttc ggcggcccca aggaagaccg cctgtgcctg acaaaagcct
      361 gctccgccaa cctgagcccg atcttcggcc tgtacgagga cgcgcgtaac gaggtcagcc
      421 gcctgctgga atcgaagctg ccggccgagc cgctcgcaac cggcaagctg gacggcgtcg
      481 agaactacat gtggggcatc agcgcacgtg aggtaatcga cgaggcggtt cggctcatgg
      541 cgccgcggcc catctatatc gccgacgggc accaccgcta cggcacgggc atgctgcacc
      601 gcgattggct catcggcgag cgcggcgaat tgccgccgga gcacccggcc aacttcgttc
      661 tgtgcgtgtt ctgcgcgatg gaagacccag ggttgctgat cctgccgacg caccgcgtcc
      721 tgagcggcgt ggttgtcaag gcggcgagtt tgcagtccga tccacagctt gaagtgcgcc
      781 aactgaacgt gtcgggcgcc gagtccgtga ttaccgcgtt gcgcaactca ggcccccagg
      841 ccgtggcctt ccacaatccc gcggaaaacg gctacttcgt ggtgcgcccg aagaacccgg
      901 accttctcga caaactggag ccggaccata gtccggcgtg gcggcgcctc gggctggcgt
      961 tcctgcacgc gtacctgctg aacaaggtga ttacaccgca gctctgcggc ggaagcgagc
     1021 cggccgtgca gtacatcaag tcggcggatg aggcggtcgc cgcggccgat cagagccagg
     1081 gcaccgtctt cctgctgcag ccgaacacca tggaagagct gcgcaacgtg tgcagtgcgg
     1141 gcgatctgat gccgcacaag agcacgtact tttatccaaa gctggccagc ggcttggtta
     1201 tcaacccgct ggatgagtag caccggctcg aggccggcgt ttcggcattc gttttcactg
     1261 tcgacgccgg ccggccgcta agcgtagcgg ccgtcgccgg gtgtaacatt ggcagccggc
     1321 ccacgcccct gttcgtgtgg ccggctgcgt gactatttgt ccatcggctt gcgcagcaaa
     1381 cgcgaataaa agaacactac caccgccacc acgacgccca acgacgacag catgaacacc
     1441 cacccgccgg ccgtcaggcc actgcccgcg agaatcattt cttgccctcc gcggcgacgt
     1501 ctgcgcacac ctcaatggca actcgattcg gcccccagcg ccggtccgcc aagtgtgcca
     1561 taaagagata gaaagccaac aacaacccta tgaggcccac ggccagcgcg ggtacgccac
     1621 cctggccgat ggagcgcaaa tggcccccca tgtagaagta gcaccacatg cctaacacga
     1681 tcagcaagag cgccggcgtg acaaaacgca tgacgtactt atacatccag gccggaggtc
     1741 gcattgcagc cccgtccagc gctacctggt agccgcgccg cgccccgaac acccacgcca
     1801 gaacgatcac ctccagcgtg cccaacacca ggatgcccac ttccgccgac cagaagtcga
     1861 gcgtgctcaa ggccgccaaa ttcttgctga accagatgac gaacaggttc cccagcaaag
     1921 tcacgacggc caggatcgta accgacgtgc gccgggtcac gttcagcgct tcctcgagaa
     1981 aggcgatcac cggctgcagc agcgataccg aactggtgat cgccgccagg aacagcatga
     2041 aaaaccataa aaagccgaac agccggccgc ccggcatatt ctgaaacacg atcggcagcg
     2101 tattgaacgc caggccgaag gtcccctttg acgctccgac gacccccagg aatatgaacg
     2161 cggccggaag ggtgattagt ccgcccaagc agacctcgaa gaactcgttc gtcgagatgg
     2221 ccgtcgtgga cgtaagcacg atatcctctt tcggcctgat ataactggcg taggtgacga
     2281 tcacaccgaa cccgaccgac aacgtgaaaa acacctgccc cgcggcggcc agccacacct
     2341 tcgggtctgc caaagcctgc agcgccgact ggccttcatg taccggcttg ggattccaca
     2401 tgaaaccgag cccatccagc acggtacgcg tctcgcccgc ctcgtgaacc gccggcaaag
     2461 tcagcacgcg gatcagcacg aagatcgcgc acacggccat gatcggcatg gccaggttgc
     2521 agaacttttc gatgcccttc gagatgccgc ggtaaatcag ccagaagttc gccgcaacta
     2581 cgatcagcca gaagtacacg ctggtgtgca cggtgccatt gaacatgacg ccgtcaaaat
     2641 tatcgcccgc gaagccgctg aagacttccg cgcttctctt ggcatactcg gccgggtcgc
     2701 tacccaaatc gatcgtcccg cacaggtatg accaggcata tcccaggcac caggattcga
     2761 tgacgacgta gtacaggtac acgaccgtgg gcatcagcag ccccaacgcg ccggcgtagc
     2821 gccaggcgcg gtgcccgccc aggtgaccga gaatcgccga acacgagtgc agccccaggc
     2881 ggccgcccgc cttgcccatg gtccattcgg cccagcagat cgggatgccc agcagcaaca
     2941 gggcgcagaa atacgggatc atgaacaccc cgcccccgtt ggccgctgcc gtggccggaa
     3001 aacgcagaaa gttccccagg ccgaccgccg agccggccac cgccataatc acgccgatgc
     3061 gcgtccccca ctgttccgtc gggcgggcca tatgccggtg ttctcctctt tgctgccggt
     3121 gcagaccgat gcgaatttgc ggccggtgca gcccggtgcg aacgttgctc ctctgcggac
     3181 tctcgtgccg gtgcggcccg cgggcacgct gctcgtagcc cacgctgcct gccaggccga
     3241 attctaagcc ctaacgcgcg cggaaaacaa tggagcttac gaccccgcac ccgtagcgtc
     3301 ggcccccgta gccggcacac gcgtagcgtc ggccccccgt ggccgacgtc aatatccagc
     3361 ccgctgggca gtctctggtc acattccggc tcggcacgag ccggctctgc tcacggacgg
     3421 gaaacggatt ccttcagttt tcggagcccg agaaggcaac ctggaacacg gccaagtcgg
     3481 acagatcgac gtccagatcg ccgtcgaaat ccgctcgtgc gaactgctcg ggcgtcgcgc
     3541 cgggcggcgg cgtggtgacg tccgggccgg ccatgcaggt cgtgaagatt gctaaatcgg
     3601 cggcatccac gtacccgtcg ccgttcaagt cggcgctgta gtccacaatt cccaa
//
LOCUS       DAOSYS010000320         3638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20501_length_3638_cov_4.180296, whole genome shotgun sequence.
ACCESSION   DAOSYS010000320 DAOSYS010000000
VERSION     DAOSYS010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20501_length_3638_cov_4.180296"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLU99_14615"
     CDS             <1..212
                     /locus_tag="PLU99_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18325.1"
                     /translation="VAPYSTRARPGAPVSTPLAWDELSPEIGPASYTVKNIPQRLAKL
                     KRDPWEKLFITRQTISARVAARLGL"
     gene            433..681
                     /locus_tag="PLU99_14620"
     CDS             433..681
                     /locus_tag="PLU99_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18326.1"
                     /translation="MESMRNRLRGWNGGTKLTQVIEEQTSRLPSDTFLWAAGAAIVTS
                     LVLQIMNRREHAQFVGQWAPTFLALGLYSKLVRHFGHE"
     gene            760..1653
                     /locus_tag="PLU99_14625"
     CDS             760..1653
                     /locus_tag="PLU99_14625"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOO18327.1"
                     /translation="MVAIQHGGAAVARNTKTEANVGQAERIASVAVGGTAALAALRML
                     VGRHPLLGVSLAAGGAYLLRRGLTGHCALYETFGVRAEDADKLSNPFTRELRTENSMT
                     VMRSPQELYRLWCDPEAIVQFLPLMERVDQHDDRHLHFVSRDPDSGQPIEWDAEITED
                     RPGQFIAWRHNKPTPFTAGQITFSPAAGERGTVVRLEFRYRVPGGVFGAAFNKLRGRS
                     VDERAREILRRFKQFAETGEVATNEGPFGARDKRAGVSLPPKAAAISAAPCPEPAARA
                     ADVVQEASEESFPASDAPAWS"
     gene            1769..2956
                     /locus_tag="PLU99_14630"
     CDS             1769..2956
                     /locus_tag="PLU99_14630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HOO18328.1"
                     /translation="MKAVCWNGKQDVRVEDVPEPRIVNPHDAIVRVSLTAICGSDLHL
                     YNGSVPTMKPGDVLGHEFMGEVVEVGRKVEKLKPGNRVVVPFAIACGQCWFCRHELWS
                     CCDNSNPNAYMLEKVAGQSTSGLFGYSHLYGGYPGGQAEYVRVPFADTGALPVPPELP
                     DERVLFLSDIFPTGYQAAEQCNISPGDTVAVWGCGPVGQFAVRSAFMLGAERVIAIDD
                     VPERLAMARRGGAETVNFTETDVHDHLMESTGGRGPDACIDAVGLEAHGRTLDAMYDR
                     VKTATYLATDRANALRQAIRACRKGGTISIPGVYAGVVDKFPIGIAFAKGLTFKMGQT
                     HVQRYLRPLLDRIKNDEIDPSFVITHRARLADAPQAYETFLSKRDSCIKVVLTPDGNA
                     QPR"
     gene            complement(3128..>3638)
                     /locus_tag="PLU99_14635"
     CDS             complement(3128..>3638)
                     /locus_tag="PLU99_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOO18329.1"
                     /translation="PALATKSDSTVLASRAPGDTAVKGAPSEDTAAKPAKPEPGPVAA
                     APADRGARAQAKSKISGGGPAPRVVSIEPANGARDVSPDLEAIVVRFDRPMMNKSFSV
                     CGSGPHFPELAGDVSFDSSRKVLTIPVKLKPDWSYEFSLNCESYRNFKSEEGIELEPY
                     KVAFRTAKK"
BASE COUNT          638 a         1193 c         1177 g          630 t
ORIGIN      
        1 ccgttgcgcc ctattcaacc cgggcccgcc ccggcgcgcc ggtttccact ccgctggcgt
       61 gggatgagct ttcgccggaa atcggtccgg ccagttacac cgtcaaaaac atcccgcagc
      121 gcttagcaaa gctgaaacgt gacccgtggg aaaagctatt tataacccgc cagacgatct
      181 cggcccgggt cgcggcccgg ctgggactgt aaaccccgcg cgtcccagcc gcgcccaccc
      241 atcaagcctt gcgcggcgcc ggccgtgacg cgggtttcat gccaaattca gtagcgcgtc
      301 agcccatctg cagatactca ttggagttgg agcgagtcgg aatggccgcc gagcacagcg
      361 gcgcggcgac taaccccggt ttactgccgg tcagtcgccg gtttttgcga atactctaag
      421 cgaggactat ccatggaaag catgcgcaac cgcctgaggg gctggaacgg cggaacaaag
      481 ctgacccaag tcatcgagga gcagacctcg cggctgccga gcgatacgtt cctgtgggcc
      541 gcgggcgccg cgattgtcac ctcattggtc ttgcagatca tgaaccgccg cgagcacgcg
      601 cagttcgtgg gccaatgggc gccgaccttc ctggcgctgg gtctgtacag caagctcgta
      661 cgtcatttcg ggcacgagta agcgccgcgg tggcctgagg cgagttcata gcggccggcg
      721 gagatgtcgc cggacgaacg gcacaagcgg agttgagata tggttgcgat tcagcacggc
      781 ggcgcggcgg tcgcccgaaa cacaaaaacg gaggcaaacg tcggccaggc tgagcgtata
      841 gcctcggtgg cggtcggagg cacggccgcc ctggcggcgc tgaggatgct ggtcggccgg
      901 cacccgctgc tgggcgtctc gctggcagcg ggcggcgctt acctgctgcg gcgcggccta
      961 accgggcact gcgcgctgta cgagactttc ggcgttcgcg cggaagacgc ggacaagctg
     1021 agcaacccgt tcacgcgcga actgcggacg gaaaacagca tgacagtaat gcgctcgccg
     1081 caagagttgt accgcctgtg gtgcgacccc gaagcgattg tgcaatttct gccccttatg
     1141 gaacgcgtgg accagcacga cgaccggcat ttgcatttcg tctcacgcga cccggacagc
     1201 gggcagccca tcgaatggga tgctgaaatc actgaggacc gtcccggcca attcatcgcc
     1261 tggcgccaca acaaaccgac gcccttcacg gccggccaga ttactttctc accggccgcc
     1321 ggtgagcgcg gcaccgtggt ccggctggaa tttcgctacc gggttccagg gggtgtcttc
     1381 ggcgccgcgt ttaacaagct gcgtgggcgc agtgtggacg agcgggcgcg cgagatcctg
     1441 cgccgcttca agcaatttgc cgaaacgggc gaagtcgcca cgaatgaggg gccatttggc
     1501 gcccgagaca agcgggccgg cgtaagcctc cctcccaaag ccgccgcaat cagcgccgct
     1561 ccgtgtccag aaccggccgc gcgcgccgcc gacgtggtcc aggaagcctc cgaggagtct
     1621 tttccggcca gtgatgctcc cgcctggagc tgatccagcg ggcgaacgta gcgtccgcta
     1681 tgcgaaccgg ctttgtgtcc gctcgtgcgg gccaagtgtg aaaatcgcgt agggtccgct
     1741 gtgcggaata ttctggccga ggtgtcaaat gaaagcagtg tgctggaacg gtaagcagga
     1801 cgtccgcgtt gaagacgtcc ccgaaccgcg catcgtcaac ccgcacgacg ctatcgtacg
     1861 cgtcagcctg accgccatct gcggctcaga tctgcacctt tacaacggct ccgtgcctac
     1921 catgaagccc ggcgacgtcc tggggcatga gttcatgggc gaggtcgtcg aggtggggcg
     1981 gaaggtcgag aagttgaaac ccggcaaccg cgtggtcgtg ccgtttgcca tcgcctgcgg
     2041 gcagtgctgg ttctgcaggc acgaactctg gtcatgctgc gataattcca accccaacgc
     2101 gtacatgttg gagaaggtgg ccggccagtc gacgtcgggt ctgttcggct attcgcacct
     2161 ctacggcgga tatcccggcg gacaggccga gtacgtccgc gtgcccttcg ccgacaccgg
     2221 cgcgctgccg gtcccgccgg agctgccgga cgagcgtgta ctgttcctga gcgacatctt
     2281 ccccaccggc taccaggcgg ccgagcagtg caacatctcg cccggcgaca cggtggcagt
     2341 ctggggttgc ggcccggtcg ggcagttcgc ggtgcgcagc gcgtttatgc tgggggcgga
     2401 gcgcgtgatc gccattgacg acgtgccgga gcgcctggcg atggcccgcc gcggcggcgc
     2461 ggaaacggtt aacttcaccg aaactgacgt acacgaccac ctgatggaat cgaccggcgg
     2521 gcgcggcccg gacgcctgca tcgatgcggt cggcctggaa gcccacggcc ggacgctgga
     2581 cgccatgtac gatcgcgtga agaccgcgac ctacctggcg actgaccgtg ccaacgccct
     2641 gcgccaggcc atccgtgcct gccgtaaagg cggaaccatc tcgatccccg gcgtgtacgc
     2701 cggcgtagtc gataaattcc cgatcggcat cgcgtttgcg aaagggctga ccttcaagat
     2761 gggtcagacg cacgtacagc gctacctgcg cccgctgctg gatcggatca agaacgacga
     2821 gatcgatccc agcttcgtga tcacgcatcg tgcccgcctg gccgatgcgc cgcaggctta
     2881 cgagaccttc ctgagcaagc gcgacagttg catcaaggta gtgctgacgc cggacggcaa
     2941 cgctcaaccc cgctaggcca cagctctaca ggcgccacgg gcgggcgccg tgggccgggc
     3001 gctgggtgcc atgccccaaa gcttgggtgc catgcccaag ctctaagcgc gggcatgccc
     3061 tcttaccgtc cgttctgact tcccggttaa gccttctgcg ttcttcgagg tgtcctcttg
     3121 tccggcctta cttcttggcc gtcctgaagg caactttgta tggctccagc tcaatgccct
     3181 cttcactctt gaagttccgg tacgactcgc agttcagcga gaattcatag ctccagtcgg
     3241 gtttgagctt gaccggaatc gtcagcacct ttcgcgacga gtcgaaactc acgtcgccgg
     3301 ccagctccgg aaaatgcgga cccgaaccgc agacggagaa cgacttgttc atcatcggcc
     3361 gatcgaaccg cacgacgatg gcttcgaggt cgggcgagac atcccgcgcc ccgttggcgg
     3421 gctcgatgga aaccacgcgc ggcgccggcc cgccgccgct gatcttgctc ttggcctgcg
     3481 cccgcgcacc gcggtcggcc ggcgccgccg ccaccggtcc gggctcgggc ttggccggct
     3541 tggccgccgt gtcttcactg ggcgcgccct tgaccgccgt gtctccgggt gcgcggctgg
     3601 ccaggaccgt gctatcactc ttggtcgcca gagccggc
//
LOCUS       DAOSYS010000321         3621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20601_length_3621_cov_6.048514, whole genome shotgun sequence.
ACCESSION   DAOSYS010000321 DAOSYS010000000
VERSION     DAOSYS010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20601_length_3621_cov_6.048514"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLU99_14640"
     CDS             complement(<1..399)
                     /locus_tag="PLU99_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO18330.1"
                     /translation="MSRINTNIPSLVAQRVLANQSASLSTSIYRLSTGYRINSGKDDP
                     AGLIASENLRCEQRAIQAAVTNISRANNIVASAEGGLVEINNLLVQLEDLVDRSANEA
                     GISQEERDANQLQVDAILESINRIANNTEFQ"
     gene            complement(671..2107)
                     /locus_tag="PLU99_14645"
     CDS             complement(671..2107)
                     /locus_tag="PLU99_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOO18331.1"
                     /translation="MARINTNVGAVIAQRHLDQSYRTLNGTLERLSTGLRINHGKDDP
                     AGLIVSERLRSETGAVAQAISNTQRASLIISTTEGALDEVAALLRDIQQLTVESANKG
                     ALSEEEIKANQLQVDSAIASIERIANSTTFAGRHLLNGSLDYITSGVVPGDIDALAVH
                     GALFGTRDYIPIEVQVIQSAQFAQLVYDGGTLSQDVTIEIQGNAGATTLSFAAGTSAP
                     KIAEAINQISDATGVSAIPLSNPGDGFTLKSAHLGSKNFVSVQLLPGSGAFALEDLSG
                     APVGTGRAYGRDATANINGAQSIGDGNKLTLKTSTLDIELSLADTFGTGTTAFTITDG
                     GALFQIGAHINSNLQVNIGVQSVAPSRLGNGAIGYLSQIVTGEQYSLVSGKSAQASQI
                     IDEAIAQVSVIRGRLGAFEKNTLQTTMNQLGITQENLMSAESAIRDADFAYETSELTR
                     NQVLVNSGTTALQLANQTPQMVLKLLGG"
     gene            complement(2192..2431)
                     /gene="csrA"
                     /locus_tag="PLU99_14650"
     CDS             complement(2192..2431)
                     /gene="csrA"
                     /locus_tag="PLU99_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOO18332.1"
                     /translation="MLVLSRQRDESIIVGDNVQITVVDIRGDKVRLGIVAPAEIPVHR
                     KEVYDAIQRENMRAAAAAGEVAGATRPPRKDGKTT"
     gene            complement(2634..3083)
                     /gene="fliW"
                     /locus_tag="PLU99_14655"
     CDS             complement(2634..3083)
                     /gene="fliW"
                     /locus_tag="PLU99_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOO18333.1"
                     /translation="MLINTTRFGPLEVDGDRIITFRDGLLGFPQLRRYALIQTSIDPI
                     FFLLQSLDDPAIAFVVCDPRAFVPDYQVPIRSDDLELLELRDLEDCQVLIIVNKVGDR
                     LTGNLLGPLVIGAHSFLGKQFVLSEKQYGTRHVLTRIPQPLPVTKTA"
     gene            complement(3373..>3621)
                     /locus_tag="PLU99_14660"
     CDS             complement(3373..>3621)
                     /locus_tag="PLU99_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18334.1"
                     /translation="LEATMKKLQEVQGRMASHAKAMEARSRLAENEATGATVLLSDLR
                     DVDLTEVIVRFQQMQTALQANLSTSSAIMNLSLIDYLR"
BASE COUNT          652 a         1078 c         1156 g          735 t
ORIGIN      
        1 ctggaactcg gtgttgttgg cgatacggtt gatcgactcg aggatggcgt caacctgcaa
       61 ctggttcgcg tcgcgctcct cctggctgat accggcctcg ttggcgctgc ggtccaccag
      121 gtcctcgagc tgcaccagca ggttgttgat ctcgaccaac ccgccctcgg ctgaggccac
      181 gatgttgttg gcccgcgaga tgttggtgac cgcggcctga atggcgcgct gctcgcagcg
      241 caggttctcc gaagcgatga gcccggccgg atcatcctta ccggagttga tgcgatagcc
      301 ggtgctcaag cggtagatcg acgtgctgag cgaggcgctc tgattagcta gaactcgctg
      361 agcaaccaag gaagggatat tggtgttaat tcggctcatg gaatgcgcct ccacgggcgt
      421 tgggcgtttt gcgggctcgg cccgcgttcg ccgttgtttt gcctcttgca tcacaaaccg
      481 gccgagcctc ccggctggcc acaactgtca gagagggaat cgtcgcgggg cgtgagaggc
      541 tttagttaaa aagcgcgggc gcagatcagg ccccggggcg cctgggtcgg gctaccccgg
      601 ggcatccggt cgcgccggcc gcgggagtgc atttgccggc atcgagaccg atatttcttg
      661 ccgaaagcgg ctatccgccg agcagtttga gcaccatctg cggggtctgg ttggccagtt
      721 gcagcgcagt ggttccggaa ttcaccaata cctggttgcg cgtcagttcc gaggtctcgt
      781 aagcgaagtc cgcatcgcgg atggcggact cggcggacat caggttttcc tgcgtgatgc
      841 ccaactgatt catggtcgtc tgcagggtgt tcttttcgaa cgcccccagc cggccgcgga
      901 taaccgaaac ctgagcgatg gcctcgtcga tgatctgcga agcctgggcg ctcttgccgg
      961 acaccagcga gtactgctcg ccggtgacga tctgcgagag atagccgatg gcgccgttcc
     1021 ccaggcgcga gggggctacc gattgcacac cgatgttaac ctgcaagtta ctgtttatat
     1081 gtgcgccaat ctggaacagg gccccgccgt cagtgatcgt gaaggcggtg gtgccagtgc
     1141 cgaaggtgtc ggcgagggac agctcgatat ccaaggtgga ggtcttcaag gtgagcttgt
     1201 tgccgtcgcc gatgctctgg gcgccgttga tgttggcggt ggcgtcgcga ccgtaggcac
     1261 gcccagtccc gacgggagcg ccggagaggt cctccagggc gaaagcgccg ctacccggca
     1321 agagctgcac ggaaacgaag ttcttgctgc cgagatgggc gctcttgagc gtgaatccgt
     1381 cgcccgggtt ggacagcggg atggcgctga cgccggtggc atcgctgatc tgattgatgg
     1441 cttcggcgat cttgggggcg gacgtgccgg cggcgaacga gagcgtcgtc gccccggcgt
     1501 tgccctggat ttcgatggtt acgtcctggg acagggtgcc gccgtcgtag actaactggg
     1561 cgaactgcgc cgattgtatg acctggactt caatcgggat gtagtcccgc gtgccgaaaa
     1621 gcgctccatg cacggccagg gcgtcgatat caccgggcac aacgccgctg gtgatgtaat
     1681 ccagtgagcc gttgagcagg tggcggccgg caaacgtggt gctgttggca atgcgttcaa
     1741 tgctggcgat ggcggaatcc acctggagtt ggttggcctt gatctcctct tcggagaggg
     1801 cgcccttgtt ggcggattcg acggtgagtt gctggatatc gcgcagcaga gcggccacct
     1861 catccagcgc gccttcagtg gtggagatga tcaggctggc gcgctgcgtg ttggagattg
     1921 cctgggcaac ggccccggtt tcactgcgca gccgctcgga aacgatcaat ccggccggat
     1981 cgtccttgcc gtggttgata cgtagcccgg tgctcagacg ctccagggtg ccgttgagcg
     2041 tgcggtaaga ttggtcaagg tgtctttggg cgatgacagc cccaacgttc gtgttaatac
     2101 gagccattag ccatcctcct tgatagggtc gtggctgtaa aagaagcaat gcggtcagtc
     2161 agaagggaaa gacgggtgat cgaaagtctg tctacgtggt tttgccatcc ttgcgcggtg
     2221 gacgcgtcgc accggcgacc tcgccggccg ccgcggccgc acgcatgttc tcgcgctgaa
     2281 tagcgtcgta aacttccttg cgatgaacgg gtatttcggc gggggccacg atgcccaaac
     2341 gcactttgtc cccccgaatg tcgacgaccg tgatttgtac gttgtccccc acgattatgg
     2401 attcgtcgcg ttgtctggag agcactaaca tccgcggctc cttcctggac ggtgccgatt
     2461 actgattcgc cagcgagcgc caacggcagc gaaagcactc gccgagtgct ggtgctccgg
     2521 tcggcagccc ggatcgattc cgccgccccc ggccgcagac gcggcccggc tagcgcgaat
     2581 cgacccggcg cgattatagc cgaacaggcg tacacaacac cactcctatt cacttacgcg
     2641 gtcttggtca ccggcagcgg ctgagggatt cgggtcagca cgtgccgggt cccatactgc
     2701 ttttccgaca ggacgaattg cttgcccagg aacgaatggg ctccgataac cagtgggccc
     2761 agcaggttac cggtcagacg atcgcccact ttgttcacga tgatcaaaac ctggcagtcc
     2821 tcgaggtctc taagctcgag caactccaga tcatcgctcc ggatcgggac ctgataatcg
     2881 gggacgaagg cccgcgggtc gcagacgaca aacgcaatcg cgggatcatc caatgactgc
     2941 aacaggaaga atatggggtc gattgaggtc tggatcagag cgtaacgccg cagttgcgga
     3001 aacccgagca aaccgtccct gaaagttatg atacggtcgc cgtccacttc caatggtcca
     3061 aagcgggtcg tgttgatgag catccttttg ctcctgccga ggctcgtgtg tcgaaagctg
     3121 cccggcggcg cccaacctgc acaggtcggt taagttccat caccgctgag cggggcgttt
     3181 ccaaccgccc gggtcgccga ggcgacccca atcacagttg ccgggtctga gcctcgccga
     3241 cccgcggcaa cctccacacg cacccttctc cgacggcccc aaattccgct acctgcctct
     3301 tcgatccgag ctctctcacc cgagcccttt cacccgagcc cgaagcgcca gcgagggcca
     3361 ccgccctggc tgtcagcgca ggtaatcaat caatgagagg ttcataatgg ccgacgacgt
     3421 ggacagattc gcttgaagcg ccgtctgcat ctgttggaag cgcacgatca cttcagtcaa
     3481 atccacgtcg cgcaggtcgc tcaacaacac cgttgccccg gtggcttcgt tttccgccag
     3541 ccgggagcgg gcttccatag ccttggcgtg tgaggccatg cggccttgaa cctcctggag
     3601 tttcttcatg gttgcttcga g
//
LOCUS       DAOSYS010000322         3604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20712_length_3604_cov_3.338405, whole genome shotgun sequence.
ACCESSION   DAOSYS010000322 DAOSYS010000000
VERSION     DAOSYS010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_3604_cov_3.338405"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..1363)
                     /locus_tag="PLU99_14665"
     CDS             complement(440..1363)
                     /locus_tag="PLU99_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18335.1"
                     /translation="MIDKPPKVETKTTSKSKRIQRGVQSPRPDAGDSGQQCSRKADRE
                     RHLAEKFFAHLGVRIERVESGPVEQAPDVKVECILDGKRRTIWVELTDYQVDQKPGGG
                     GSPARLTCNVGRRTLDRAQEIFAGQCPSISLEVRVRFDHEALPRKDQIEPLAQELAAF
                     LRDHAAQGCRQPITFNRRPRAFDAYPLLQSHVESIILFRTSHLPYWQLNNARHIHLSP
                     EILADRISSKNEKRAGYKGIQAGEDCWLVIVASGETSADRAGPEIAAAGIVDNPAVLQ
                     AAGQGAFERIYFWEAVRNWHRLIWPAESAAD"
     gene            1716..2069
                     /locus_tag="PLU99_14670"
     CDS             1716..2069
                     /locus_tag="PLU99_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18336.1"
                     /translation="MSAELTPTRFVQKWSRIDLTERAALQKHFLDLCRPLGQPTPAET
                     DAIGEEYTFEKGVAVTDPEGGWSEDSAEVWTETGAGQPFPPNHPLAARRQEIDQQVLA
                     NLLRLNSIRAGLKRT"
     gene            2382..2606
                     /locus_tag="PLU99_14675"
     CDS             2382..2606
                     /locus_tag="PLU99_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18337.1"
                     /translation="MTSVQQDIEILPSRSLTDEDFQTRTTCEIASGMIVVPADPTSAD
                     RMHLIEASGTLDFWTAPDEDTYDEHDGEPV"
     gene            2626..2754
                     /locus_tag="PLU99_14680"
     CDS             2626..2754
                     /locus_tag="PLU99_14680"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOO18338.1"
                     /translation="MVFPFTDTTGGKLRPALVVSGPKFNQDEDFTAVPISSRFLRE"
     gene            2705..2941
                     /locus_tag="PLU99_14685"
     CDS             2705..2941
                     /locus_tag="PLU99_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18339.1"
                     /translation="MKTLLLSPLALGFCENDPYGFPIRASEKYFGQTGLKCDSCVKWT
                     KLITISAQVVARKLGKLPPEVLQSICDRIKGIFS"
BASE COUNT          705 a         1123 c         1043 g          733 t
ORIGIN      
        1 ggatcgaggg atcaagaagt gtaaagagca gagccggggc gtgccgcccc ggaataccgc
       61 caacaacgtc ccacccgcgc agtcgtccgc cctcgcgcct gcccctgccg ccgcgaaatg
      121 ctggcgagaa ccttccatcc gggcaggtgc ccatcccgtc cgcgtgctgt gcaatctatg
      181 gaggtctttt ttttctcaga tttcacagat tttcacagat tcggttgagt tgcggttggc
      241 cgtaggcttg cgtacccccg cacgcgcgtt gcactaagaa ctagacccca aacttgcaaa
      301 cgtcgcgaga attgtcttcg agccacactt gtgggcatcc tctggtggag caagcggcct
      361 gaagaccgga gttcttccaa ctacaccccc ttcacatgcc ggcgcacgtg atcgccttcc
      421 gcggcgtggc gtgcgaacat cagtccgcgg ccgactctgc aggccagata agcctgtgcc
      481 aattacgtac agcctcccag aagtagatgc gctcgaacgc gccttgcccc gcagcttgga
      541 gaaccgcagg gttatcaaca ataccggcgg cggcgatttc tgggcccgca cggtcggccg
      601 atgtttcacc gctagccacg atcacaagcc agcagtcttc gcccgcttgt atccccttat
      661 agccggctcg tttttcgttt ttgctactta tcctgtccgc gaggatttcg ggcgagaggt
      721 ggatatgccg ggcgttattg agttgccagt aaggcaggtg cgaggttcgg aaaagaataa
      781 tgctctcaac atgactctgg agaagggggt acgcgtcgaa cgcacgcggt cgccggttga
      841 atgttatggg ctgacggcac ccctgcgctg cgtgatcgcg caggaaagcg gcaagttcct
      901 gggcaagcgg ttcgatctgg tctttgcgag gtagcgcctc gtggtcgaaa cgaaccctca
      961 cttccagcga aatcgacggg cattgcccgg cgaaaatctc ttgggctctg tcgagtgtcc
     1021 tgcgcccaac gttgcaggtg agacgtgccg gactaccccc gccgccaggt ttttgatcga
     1081 cctgatagtc cgtcagttcc acccaaatag tgcggcgttt tccatctagg atgcattcta
     1141 cctttacgtc cggtgcttgt tctacagggc ctgactctac acgctcgata cgcacgccta
     1201 ggtgcgcgaa gaacttctcg gcgaggtgcc tctcccggtc cgcttttcga gagcattgtt
     1261 gccctgagtc accagcatct ggacgcgggc tctggacccc cctttgtatc cgtttcgatt
     1321 tcgacgtcgt cttcgtctcc acttttggtg gcttgtcaat catgtcgagc acctcgtgcg
     1381 gcggaatatg tagagcgcgg ccctgcaatt ttcaattatg taatgtatgt tacctgcgaa
     1441 cggcaacgcg tattagctat cagcatttcg gccgaagagg cgattcgccc gcgcaccgga
     1501 tgccgggtgc gacaatgggc tcccgcagtg gcggcggcgc cgcacgcagt cgccctaacc
     1561 gcgaacgtcg cgtgacggga gtattgatag accggggttg cggaaaggat tccgcgggag
     1621 tgtgtgcgag acggattctc gcacgagcga gcgcatgccg cactgccgct gggcttctgg
     1681 acggtggcgg gctgaaactc cacaatgtac cctaaatgtc cgccgaactg acgccgaccc
     1741 ggttcgtcca gaaatggtcg cgtatcgacc ttaccgaacg cgcggccttg cagaaacact
     1801 ttctggacct ctgccgtccg ctgggccagc ctaccccggc ggaaaccgac gctatcggcg
     1861 aggaatacac attcgagaag ggtgtcgccg ttaccgaccc ggaaggcggc tggtccgaag
     1921 actcggccga agtctggacc gaaaccggcg ccggtcaacc gttcccgcct aaccaccccc
     1981 tcgccgcccg gcgccaggaa atcgatcagc aggtgctggc gaatttgctt cgccttaaca
     2041 gcatccgcgc cgggttgaaa cggacgtgac gctccatccc ctagcccgct gaccaagcgc
     2101 cgtggcggcc acgtgcgccc agcccgataa cgagtacagg cgcggccgca gtacacaaaa
     2161 actcaacatg gcccggattt gcgtgtgtac aatttcgcga tgaacagacg agctaggccg
     2221 cccaagtcgg ccgagcacaa gcgcgcgcag cgcctcgacc tgcgactacg tacggcagaa
     2281 aagcaggcgt tcaggttggc ggccgaaagt tccagcggcc gctcaacgtc gtcgaatcag
     2341 gccggatagc aatgtagggc cacaataaac ataggtgcaa tgtgacatcc gtacagcagg
     2401 acatagaaat tctgccgtcg cgcagcctga ctgacgagga ttttcagacg cgcacaactt
     2461 gtgagatcgc atcagggatg attgtcgttc cggctgaccc caccagcgcc gatcgaatgc
     2521 atttgatcga agcatcgggc acgctcgatt tctggaccgc accggacgag gatacgtacg
     2581 acgagcacga cggcgaacca gtttgagccc gggcaagtcg tcctcgtggt ttttccgttc
     2641 actgacacga ctggggggaa gctgcgcccc gcgctggtgg tttctgggcc caaattcaac
     2701 caggatgaag actttactgc tgtccccatt agctctcggt ttttgcgaga atgatcccta
     2761 cggctttccg atccgcgcat cggagaagta tttcgggcaa accggcctga aatgcgattc
     2821 gtgcgtgaag tggaccaagc tcataacgat tagcgcccaa gtggttgcac gaaagctcgg
     2881 aaagcttccg cccgaggtcc tgcaatcgat ctgcgacaga atcaagggga tattttcctg
     2941 aacatcgcgg cgaccgcccg gccatgctcg gggagaccgg gactggttta cttgttggtg
     3001 cgggctgcgc gcgtacggcg ggaacgcaag cgttcggccg accgcaggcc aatcgagtct
     3061 gggcgaatct gcgaaatctg aggagagaac acgcccccct tgcgcccgga gtccgggccc
     3121 ttgcgcccgg agctgcccca gttgcgcccg gagttccagc agctgcgcgc ccagctcagc
     3181 tctgagccct cagctttcag cacgagccgc acgccgacgg agttaaaccc tgttcttggc
     3241 ggccttggcg tcttggagtc tcgtcttggc ggccttggcg ctcgtggcgt cttggcggtt
     3301 gcctttgctg agagctttct ttatcaccct atcgccttat cactttatca tcccaagctg
     3361 cgcccacgcc gccactggcg cgcagccacc cacaatccgc gcattccggc cgaaagctga
     3421 catctgatcg ctgccgcggc ctagtatatc tagaagaacg cagccgtaac cgacactgac
     3481 aacgagaaac gcgctgcccc gcggaggtcc gcccatatgc ctgtcagaca agattgcccg
     3541 tcttcggcgc ctctcctgtt cacctttcac ctctctcctg ctctcgcgca gcgagttatc
     3601 ggca
//
LOCUS       DAOSYS010000323         3568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_20959_length_3568_cov_3.615144, whole genome shotgun sequence.
ACCESSION   DAOSYS010000323 DAOSYS010000000
VERSION     DAOSYS010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20959_length_3568_cov_3.615144"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLU99_14690"
     CDS             complement(<1..374)
                     /locus_tag="PLU99_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18340.1"
                     /translation="MNRVKLGYYVLRYLGPKFALRRAALYARHKAGWDRRLFAPRDWD
                     QIPVPLPEAAASSSAQTNSAEASRNQPTSAETYANWKTANSPPFFFPLGQPPKLPVEL
                     RSTATGRQPNLAERIRLLQQGR"
     gene            complement(371..2563)
                     /locus_tag="PLU99_14695"
     CDS             complement(371..2563)
                     /locus_tag="PLU99_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOO18341.1"
                     /translation="MRQVLQHLRTGQIECADLPAPLARPGTLLIRTSRSLISAGTERS
                     LVEFARSGWIGKARSQPEKVRQVLDKIKTDGLMPTLETVFARLDEPMPLGYCNAGVVL
                     EVGPGVTGFAPGDRVASNGSHAELVCVPPNLCAKIPDGVSDEEAAFTVLSSIALQGIR
                     LVQPTLGENIVVYGLGLIGLIAVQLLAASGCNVLGIDVHPDRLRLAQKFGARTFDASG
                     GASPVAAGNSWTSGKGVDGVLITASAQTDEIMHAAAQMCRKRGRIVLVGVVGLNLRRP
                     DFYEKELTFQVSCSYGPGRYDERYEQGGQDYPYAYVRWTEQRNLEAVLAAMQAKRLLV
                     HDLITHRYPLAEAPAAYATIRYDSSSLGVILEYPAEVSRAPRINIPARTSAAAGQAVV
                     GIIGAGNFAKLVMGPNVAKAGARLKYVADVNAAAAQYLARKCGGEQAVTDYRLLLEDP
                     EVKAVLIAVGHSLHARFVCEALAAGKHAFVEKPLAMNPAELGQVMRTAAEHPDRLVMV
                     GFNRRFSPHALKMKSLLAGRSEPLCMNMTVNAGYIPPEHWVQDPVRGGERIIGEGCHF
                     IDLLSFLAGSQVRTVAALMAGEGVATRSDKMSIVLGFEDGSVGTVNYFANGSKAYPKE
                     QLEVFSEGRVLRLDNFRRVSGYGFGKFKQLKSVRQDKGHAAEFAAFVQRVTTGGPALI
                     PLDELVNVTLATFAAMTAARERRTIDLAAEYGEALGVCLRPASAITTA"
     gene            complement(2560..>3568)
                     /locus_tag="PLU99_14700"
     CDS             complement(2560..>3568)
                     /locus_tag="PLU99_14700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO18342.1"
                     /translation="GMLRRWMFETYRLPRLVRDFRPDVVMALAGRGLLRPGCTQVAFP
                     QDSHFYYPLRQFGRETYFNILKKRYHAWHFGRQLRRTQLVLIQTPVVAQHLRDRFGYR
                     GATFVCSTSVTPALREGRRGRPVPTALRALEGKFKLLYLTRYYAHKNLEAAVPLFREF
                     ADDLRDVVLITTVRADHHASAPAFLREIERYGLRDRIVNVGPIPHDEVGAYYENVDAL
                     FMPTLLETFGIPYLEAMCFDLPVLTSDLDFAHAVCGEAALYFDPFSPRSMRDAILRVK
                     NDSELRSKLTDAGRARLAEQFPDWDTLASGILEQVRRVLMPGPPGPMRAADSEVSMEP
                     I"
BASE COUNT          597 a         1151 c         1189 g          631 t
ORIGIN      
        1 cagcggcctt gctgcagcag gcggatgcgt tccgcgaggt ttggttggcg gccggtggcg
       61 gttgaacgca gttcgacggg aagttttggt ggctggccga gcgggaagaa gaagggaggt
      121 gaattggctg ttttccagtt ggcgtaggtt tctgcgctgg ttggttgatt gcggcttgct
      181 tcggcgctgt tcgtttgcgc actgcttgaa gctgcggctt caggcagtgg cacagggatt
      241 tggtcccaat ctcgaggcgc gaagaggcgc cggtcccacc ccgccttgtg ccgcgcgtac
      301 agggcggctc gccggagggc gaacttcggc cccagatagc gcagcacgta atagcccagt
      361 ttgacgcggt tcacgccgtc gttatagccg acgccggccg caggcacacc cccagcgctt
      421 cgccatactc ggccgccagg tcgatggtcc gccgctcgcg ggccgccgtc atggctgcga
      481 aagtggccag cgttacgttc accagttcgt ccagagggat cagtgccggg ccgccggtcg
      541 tgacgcgctg cacaaaagcc gcgaactccg ccgcatggcc cttgtcctgg cgcaccgact
      601 tgagctgctt gaacttgccg aaaccatagc cgctcacccg gcggaaattg tccagccgca
      661 gaacgcggcc ttcgctgaag acctccaatt gctccttggg ataggctttt gacccgttgg
      721 cgaagtagtt gaccgtgccc acgctgccgt cttcgaaccc gagcacgatc gacattttgt
      781 cggagcgcgt ggcgacgccc tcgccggcca tcagcgcggc gaccgtccgg acctggctgc
      841 cggccagaaa gctcagcagg tcgatgaagt ggcagccttc gccgatgatc cgctccccgc
      901 cgcggaccgg gtcctggacc caatgttccg gcggaatgta gccggcgttg accgtcatgt
      961 tcatgcacag cggctcgctg cggccggcca gcagcgactt catcttcagt gcgtgcgggc
     1021 tgaagcggcg gttgaagccc accatcacca agcgatccgg atgctcggcc gcggtacgca
     1081 tcacctggcc gagttcggcc ggattcatgg ccagcggctt ttcgacgaag gcgtgtttac
     1141 ccgcggccaa agcctcgcag acgaagcgcg cgtgcaggct gtgcccgacc gcaatgagga
     1201 ccgccttcac ctccgggtct tccagcagca agcgatagtc ggtgaccgcc tgctcgccgc
     1261 cgcacttgcg cgccaggtac tgcgccgccg ccgcgttcac atcggcgacg tatttcaggc
     1321 gcgcgccggc cttggccacg ttggggccca tgaccagttt tgcgaagttg cccgcgccga
     1381 tgatgccgac cacggcctgc ccggcggcgg cggacgtccg ggcagggatg ttgatacgcg
     1441 gcgcccggct gacctccgcc ggatactcca ggatgacgcc caggctgctc gaatcgtagc
     1501 ggatcgtcgc ataggccgcc ggcgcttcgg ccagtgggta gcggtgcgtg atcaggtcgt
     1561 ggaccagcag ccgcttagcc tgcatggcgg ccagcaccgc ctccaggttg cgctgctcgg
     1621 tccagcgcac gtaggcgtag gggtagtcct gtccgccctg ctcgtagcgt tcgtcatagc
     1681 ggcccgggcc gtacgagcac gagacctgga acgtgagttc tttttcgtag aagtcggggc
     1741 gccgcagatt gagtccgacc acgccgacca gcacgattcg gccgcgtttg cggcacatct
     1801 gggcggcggc atgcatgatt tcgtcggtct gggcggaagc ggtgatgagc acgccgtcga
     1861 cgcccttgcc ggacgtccac gagttgcccg cggcgaccgg gctggcgccg ccggaggcgt
     1921 cgaacgtccg cgccccgaat ttctgcgcca agcgcaggcg gtcggggtgc acgtcgatgc
     1981 ccagcacgtt gcagccgctg gcggccagca gttgcacggc gatgagtccg atcaggccga
     2041 gtccgtagac gacgatgttt tcgcccaagg ttggctgaac caggcggatg ccttgcaggg
     2101 cgatgctgga aaggacggtg aaggcggcct cttcgtcgct gacgccgtcg ggaattttcg
     2161 cgcacaggtt gggcggcacg cagaccagtt ccgcatgcga gccgttactg gcgacgcggt
     2221 cgccgggcgc gaagccggtc acgcccgggc cgacttcgag cacgacgccg gcgttgcaat
     2281 agccgagcgg cataggttca tcgagcctgg cgaagaccgt ttcgagcgtg ggcatgagac
     2341 cgtcggtttt gatcttgtcg agcacctggc gcactttttc gggctgggag cgggcctttc
     2401 cgatccagcc cgagcgggcg aattcgacca gcgagcgttc ggtgccggct gaaataaggg
     2461 agcggctggt gcggatcagc agcgtgccgg gccgtgccag cggggcgggc aggtcagcgc
     2521 actcgatctg tccggtgcgc aggtgttgca gaacctgtct catatgggct ccatgctcac
     2581 ttcagagtcc gcggctcgca tcgggcccgg cggaccgggc atcagtacgc gccgaacttg
     2641 ttcgagaatg ccgctcgcca gcgtgtccca gtcggggaac tgttccgcca gccgcgcgcg
     2701 cccggcgtcc gtcagcttcg aacgcagctc ggagtcgttc ttcacccgca ggatggcatc
     2761 acgcatggac cggggactga acgggtcgaa gtacagcgcc gcctcgccgc acaccgcgtg
     2821 cgcaaaatcc agatcactgg tcaataccgg caggtcgaag cacatcgcct ccagataagg
     2881 gatgccgaag gtctccaaca gcgtgggcat gaacaaggcg tccacgttct cgtagtatgc
     2941 gcccacctcg tcatgaggta tgggccccac gttgacaatc ctatcgcgca acccgtagcg
     3001 ctcgatttcc cgcagaaaag ccggtgcgct ggcgtggtgg tcggctctga cggtcgtgat
     3061 cagcacgacg tcgcgcaggt catccgcgaa ctctcggaac agcggcaccg ccgcctcgag
     3121 gttcttgtgc gcgtagtagc gcgtgagata aaggagcttg aacttgccct ccagggcgcg
     3181 tagcgccgtc ggcaccggtc ggccccgccg tccctcccgc agcgccggcg tcacagacgt
     3241 gctgcatacg aaagtcgcgc ctcgatatcc gaagcggtcg cgcaaatgct gcgccactac
     3301 cggagtctgg atcaagacga gttgcgtccg ccgcagttgc cggccaaagt gccaggcgtg
     3361 atagcgcttc ttgagtatgt tgaagtaagt ctcccgcccg aactgccgca acggatagta
     3421 aaaatgcgag tcctggggga acgcaacttg cgtacagccg ggacgcaaca atccccgtcc
     3481 cgccagcgcc atgaccacgt ccggccgaaa gtcacgcacc agccgcggta ggcggtatgt
     3541 ttcgaacatc cagcgccgca gcatcccg
//
LOCUS       DAOSYS010000324         3545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_21122_length_3545_cov_5.796562, whole genome shotgun sequence.
ACCESSION   DAOSYS010000324 DAOSYS010000000
VERSION     DAOSYS010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21122_length_3545_cov_5.796562"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLU99_14705"
     CDS             complement(<1..387)
                     /locus_tag="PLU99_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOO18343.1"
                     /translation="MSTILENLKQAFAGESQANRKYMAFAQQAEKDGFPNIARLFRTT
                     AEAENIHAKGHLAAMDGIGNTAQNLQAAIDGETYEYTEMYPPMVELAQREQHRCKRMM
                     EFAVKAEAVHARLYQMALEAAKAGKDL"
     gene            complement(590..1783)
                     /locus_tag="PLU99_14710"
     CDS             complement(590..1783)
                     /locus_tag="PLU99_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOO18344.1"
                     /translation="MPEKIRVGVIGCGGRGTGAAIDCMKADPGVEIVALGDLFADQLE
                     KSRRKLYEAGSQVQLAEERCFSGFDAYKGVLESNVSLVILAAPPAFRPLHFQAAIEAG
                     KHVFMEKPVAVDPVGVRSVIASSELANQNGLSVVAGTQRRHQARYLEVMKRVRNGEIG
                     DIVGGQCYWNQEGLWHERAKTNWEKYQAKQCCDMEWQIRNWLFICWLSGDHIVEQHVH
                     NLDVMNWALGETPAKAFGEGGRQARTGPEWGNVYDHFAVEYEYPNGARIFSMCRQIDK
                     ASTRVTERIVGTRGIAVPGEGKIFRRGKLVYEHQGDETNPYVQEHVDLIASIRNGKPL
                     NEGRCVAESTMTAVMGRMSAYTGRELSWDWAMNASKLNLCPPEFKMGDCPLCPVPVPG
                     QVELV"
     gene            complement(2043..2960)
                     /locus_tag="PLU99_14715"
     CDS             complement(2043..2960)
                     /locus_tag="PLU99_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOO18345.1"
                     /translation="MNRRTFLASAVAGVTASATLATGALRQPQPKRGQCFKLNYAPHF
                     GLFRHLAGDDLVDQLKFAADQGFTAFEDNGMAGRPVEEQEKIASAMERLNLIMGVFVA
                     CGSFNEPTFVRDDAETRGRLVAQMKSAVEVAHRVNAKWCTVVPGCFDPRVDWEYQTVN
                     VIDNLKACAEVCEPAGLVMVLEPLNWRDHPNLFLTRISQAYRICRAVNSPSCKILDDL
                     YHQQVTEGNLIPNLDQAWSEIGYVQVGDNPGRCEPGTGEINYHRIFRHLHAKGFQGVV
                     GMEHGKATNSREGEQAVIDAYRACDDFEA"
     gene            complement(3110..3418)
                     /locus_tag="PLU99_14720"
     CDS             complement(3110..3418)
                     /locus_tag="PLU99_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18346.1"
                     /translation="MTAIPPNWLTSITGTQGAQHQSSAHHVKETADQARRAGATPFAD
                     SLHNVIDNKDQDGQVFTDAEGTGSQGRPFTDEQQSASADTSDAPADSGSDAGHIDIEA
                     "
     gene            complement(3427..>3545)
                     /locus_tag="PLU99_14725"
     CDS             complement(3427..>3545)
                     /locus_tag="PLU99_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOO18347.1"
                     /translation="GETLDLSESRVSQMHSAILARLRNRLQARRRDFFTEPV"
BASE COUNT          605 a         1084 c         1100 g          756 t
ORIGIN      
        1 gaggtccttg ccggccttgg cggcctcgag cgccatttgg tacaagcggg cgtggacggc
       61 ctcggccttg accgcgaact ccatcatgcg tttgcaacgg tgctgctcgc gctgcgccag
      121 ctcgaccatg ggcggataca tttcggtgta ctcgtacgtt tcgccgtcga tggcggcttg
      181 cagattctga gcggtgttgc cgatgccgtc cattgccgcc aggtgacctt tggcatggat
      241 gttctcggcc tccgcggtag ttcggaaaag ccgggcaatg ttggggaagc cgtccttttc
      301 ggcctgttgg gcgaacgcca tgtacttgcg gttggcctga ctttcgccgg cgaaggcctg
      361 tttaagattt tcgaggatgg ttgacatagg ttttccttta gctgccctaa gaaccgggtg
      421 ggcccgcgac tccgcggtcg aggcgaattt tattcgcggc ctgcaaagga tgaaagtcga
      481 cctgatcgtc tcgttcacgc gccgctatcg ataacgaatc acctgccgtc aaggtgaatg
      541 gcggccggct gttgaaatcg caaagcaccc gcggcggcgt gtgcgtgcgc tacaccagct
      601 caacttgccc cggcaccggc accgggcata gcggacaatc gcccatcttg aactcgggcg
      661 ggcagaggtt cagctttgac gcgttcatgg cccaatccca cgacagctcg cgcccggtat
      721 atgccgacat gcgtcccatg acggcggtca tcgtgctctc ggccacgcag cggccttcgt
      781 tcagcggctt gccgttgcgg atgctggcga tcagatcaac gtgctcctgc acgtacgggt
      841 tggtctcatc gccctggtgt tcgtacacga gcttgccgcg gcggaaaatc ttgccctcgc
      901 ccggaacggc tatgccgcgc gtgccgacga tgcgctccgt tacccgtgtg gacgctttat
      961 caatctggcg gcacatactg aagatgcgcg cgccattggg atactcatac tcgaccgcga
     1021 aatggtcgta gacgttgccc cactcaggac cggttcgcgc ctgacgaccg ccctcgccga
     1081 acgccttggc cggcgtctcc cccaaagccc agttcatgac gtccaggttg tggacgtgct
     1141 gctccacgat gtgatcgccg gagagccagc agatgaatag ccagttgcgg atctgccact
     1201 ccatgtcgca gcactgcttg gcctggtatt tctcccaatt ggttttggcg cgctcgtgcc
     1261 acagcccctc ctggttccag tagcactgcc cgccgacgat gtcgccaatc tcgccatttc
     1321 gcacgcgctt catcacttcc aggtagcggg cttgatggcg gcgctgcgtg ccggcgacta
     1381 ccgacaggcc gttctggttc gccagctccg aggaggcgat taccgagcgc acgccgaccg
     1441 gatcgaccgc gaccggcttc tccatgaata cgtgcttgcc ggcctcgatg gccgcttgaa
     1501 agtgcaatgg gcgaaacgcg ggcggagcgg ccaggatgac caggctgacg ttggactcca
     1561 gcacgccctt atacgcatcg aagccgctga agcagcgctc ctcggcgagc tgaacctgcg
     1621 agcctgcctc gtataacttg cggcggcttt tttcgagttg gtcggcgaac aggtcgccca
     1681 gggccacgat ctccacgccc ggatccgcct tcatgcagtc tattgcagcg ccggtgccgc
     1741 gcccgccgca gccgatcacg cccacacgaa tcttctccgg cacccggtga ctcgtaatca
     1801 cagccgggcc cgtacgtttt gtgttcacag cacacccgcc ggtcagcgcg gcggctgaga
     1861 gaactgcaga agtctgaaga aactggcggc gtgagaacgg ccggccggct ggggcctcgg
     1921 agtggtttgg ttgggtcatg atgcggatct ccggagtcag ttggcaattg ttagttgtca
     1981 gttgttagtt gttagttgtt agttttggcc cttgtactct ttactgacac tgtgattggc
     2041 aactatgcct caaagtcgtc gcaggcgcga taggcgtcaa tgaccgcttg ttcaccttcg
     2101 cggctgttgg tcgccttccc atgttccatt cccaccacgc cttggaagcc tttggcgtgc
     2161 agatggcgaa agattctgtg gtagttgatt tccccggtgc cgggctcaca gcgaccgggg
     2221 ttgtcgccga cctgcacata accgatttcg ctccaagcct gatccagatt agggatgagg
     2281 ttgccctcgg tcacctgctg atgatacagg tcgtcgagta tcttgcagga tggactgttg
     2341 acggcccggc agatgcggta agcctggctg atccgcgtga gaaacaaatt cggatgatcg
     2401 cgccaattga gcggttcgag caccattacc agacccgcgg gctcgcacac ctcggcgcac
     2461 gctttgagat tgtcgatgac gtttacagtc tggtattccc aatccacgcg cggatcgaag
     2521 cacccaggga caaccgtaca ccacttggcg ttgacgcggt gggctacctc gaccgcggac
     2581 ttcatctgcg ccaccagcct gccgcgcgtc tcggcgtcgt ccctcacgaa cgtcggctcg
     2641 ttgaagctgc cacaggccac aaacacgccc ataatcaagt tgagccgctc catggcactg
     2701 gcgatctttt cctgctcttc caccgggcgg ccggccattc cattgtcctc gaaggccgtg
     2761 aagccctggt cggccgcgaa tttcagttgg tcgaccaaat cgtctccggc caggtgccga
     2821 aagagcccga aatgtggagc gtaattgagc ttgaaacact ggccgcgttt gggttgcggc
     2881 tgccgcagcg cgcctgtggc cagggtcgcc gacgcggtca cgcctgccac agcgctcgcc
     2941 aggaaagtgc gccgattcac cgatccgcct ccgttcaacg ctgatggtaa cgtacgcgaa
     3001 gcagcgtaaa ggcgtcacct acggtggtca agcggaaacg tcgtattggc gcttaatagg
     3061 aggccgcctg gacctgctgc cgcttcgatg gcgcacttat tggctgactt caggcttcga
     3121 tatcgatgtg gccggcgtcc gagccggaat cggcgggcgc gtcggatgta tcggcggagg
     3181 ccgactgctg ctcgtcggtg aaagggcggc cctggctgcc ggtgccttct gcgtccgtga
     3241 aaacctggcc gtcctggtct ttattatcga tcacgttgtg caggctgtcg gcgaagggtg
     3301 tagcgccggc gcggcgagcc tggtccgcag tttccttgac gtggtgagcg ctggactggt
     3361 gctgggcccc ctgtgttccg gtgatggaag tcagccagtt gggggggatg gcagtcacag
     3421 gcgcacctac acaggttcgg tgaagaagtc ccggcgccgg gcctgaagcc ggtttcgcaa
     3481 gcgcgccagg atggcggagt gcatctggct gacgcgtgat tccgagagat cgagcgtttc
     3541 gccga
//
LOCUS       DAOSYS010000325         3536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_21173_length_3536_cov_5.156564, whole genome shotgun sequence.
ACCESSION   DAOSYS010000325 DAOSYS010000000
VERSION     DAOSYS010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21173_length_3536_cov_5.156564"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLU99_14730"
     CDS             <1..315
                     /locus_tag="PLU99_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18348.1"
                     /translation="GIILPLGVFFPETNVPVLFNPFFFPVTRGDDLANVNAVTTNTVD
                     FNPATVPNFNSFIVNANRQTQAPPAFNFNGVFDLDFTGVFNLNNAIPPTTTTTVQNTS
                     TP"
     gene            complement(402..968)
                     /locus_tag="PLU99_14735"
     CDS             complement(402..968)
                     /locus_tag="PLU99_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18349.1"
                     /translation="MVYRLILPLLSAAVLLGGLTSATAADRDWNKNQRATFRGDAAYH
                     RQVQSVRDGQRGYTWRGGSDWGWRDGRYYGGRNFSWGWAGPRYYSGPVRHYSYSTPYY
                     HSYVYRPPVYSYSYSAPYTYYYRPYTYTYSYVPYRYAAYPDTGFVYGYAPYSYPYSYA
                     AYPGVTFRFDWDDDDGYWKWDNGWEWED"
     gene            complement(1143..1691)
                     /locus_tag="PLU99_14740"
     CDS             complement(1143..1691)
                     /locus_tag="PLU99_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18350.1"
                     /translation="MHKKQRTQTKPWLKKRGPRWSLIGLMAVGLLLVVGLGTFAVATI
                     GGKGDLNAVLRKAQLGAIPSGATDLQYAGVADSTYYLRFTASPEEIERFIADSPRLKV
                     RAPQLLGPAQMYLPDSQREANPSEGTATIYYAANERIPWFDPQVREKGRMYAIAPDRH
                     SNWTEVVIDDQKNTVYIRVRQR"
     gene            complement(1691..3349)
                     /locus_tag="PLU99_14745"
     CDS             complement(1691..3349)
                     /locus_tag="PLU99_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO18351.1"
                     /translation="MTASQREGTVFAQAARRRKIRLINPNSPLSNITMPKIIRQMTFN
                     RRALFAPTGLTICAAVVPRHWDVELIDECTLDLPHQPRADVDLVGISAMTPQAPRGYE
                     LADAYRRLGVTVVMGGIHPSAMPSEALQHCDAVCQGDAESTLPHLIRDWEEAANRGMD
                     PRQGMRRIYDWATFETAPIATPRKDIYNPADYLVANPIQTTRGCPHNCNFCTTPAVFG
                     RKYRQREIADIVEEIRAACERRRPWCFIFADDNFGGNHNWAIDLCEALRPLKVAWATQ
                     CDILISRNDRLLRTMRESGCVGLILGLESPKQDTLAVAGKKFVQSTTYAERIRKIQSF
                     GISLWGAFIFGFDTDTWQDCMRACRFAQRMNLAMSCYPILTPYPGTAFYDRFEREGRL
                     RTRDWLRYNGASVVFEPKQMSAKELRHAQMAAFAEFFSPRSALRRLRLFPLKSRAWVA
                     NLAIWKGIRYYYGQKGRQVPRFRDFLDRDARAWDYPDEEWAETCAAPGGRRALWLPAD
                     QERTDEQGGEPANLEAVALAAAAASNPIVQMAGAFANETGEVDL"
BASE COUNT          666 a         1169 c          983 g          718 t
ORIGIN      
        1 gggatcattc ttccgctcgg cgtttttttc ccagagacga atgttcctgt tttgtttaac
       61 ccgttcttct tccctgtcac ccgcggcgac gacctggcga acgtcaatgc cgtgacgacg
      121 aataccgtag atttcaaccc cgccaccgtc ccgaatttca attcgttcat cgtaaatgca
      181 aacaggcaaa cgcaggcgcc ccccgcgttc aatttcaacg gtgtgtttga tctagacttc
      241 acaggcgtgt tcaacctaaa caacgcaatt ccgccgacga ccacgactac cgttcagaac
      301 acctccaccc cgtaagaccg tgacgttata acaactactc gccctcggtc gccgccagag
      361 cggccgaggg cgatggttgc cagaactggc cgccgcctac atcaatcctc ccactcccag
      421 ccgttatccc acttccagta tccgtcgtcg tcgtcccagt cgaaacggaa ggtaacgcct
      481 gggtacgccg catagctata tggatagcta tatggcgcgt aaccgtaaac aaacccagtg
      541 tccgggtagg ccgcatacct gtatggcacg tagctgtaag tgtaagtata ggggcggtag
      601 tagtacgtat agggcgcact gtaactgtag gagtaaacag gcggccggta gacgtagctg
      661 tgatagtacg gcgtggagta cgaataatgg cggaccggcc cggaatagta gcgcggtccg
      721 gcccagcccc aagagaagtt tctgccaccg tagtagcgcc cgtcacgcca gccccagtca
      781 ctaccaccgc gccaggtgta accgcgctgt ccgtcgcgaa ccgactgcac ttggcggtgg
      841 taggccgcgt cgcctcggaa ggtggcgcgc tggttcttgt tccagtcacg atctgcagcc
      901 gttgcggacg tcaacccacc cagcagcaca gccgctgaaa gcaacggtag tatcagccga
      961 tacaccataa catgtctccc gtgatttccg gctcgttgtt cgctgatctc ggacaaaacc
     1021 gacttgcttg atgctagcta tcggcgattg agcgggtgct gaaaaactat tcaagctgtg
     1081 gcgtcgcgcg caccatcccg gcccccctta tttgcccatt tgccccaccc aggtttcccg
     1141 cgctacctct gccttacccg aatgtaaacc gtgttcttct ggtcgtctat tacaacttcc
     1201 gtccagtttg aatgccgatc cggcgcaatg gcgtacatgc gccccttctc gcgcacctgt
     1261 ggatcgaacc atgggatgcg ttcgttcgcg gcgtaataga tagttgcggt gccttcgctc
     1321 ggattcgctt cacgttgcga atcgggcaag tacatttgcg ccggcccaag caactgcggc
     1381 gcacgtactt tcagccgcgg agaatccgct atgaaacgct cgatttcttc gggcgatgcg
     1441 gtgaatcgca ggtaataagt gctgtccgcg acgccggcat actgtaagtc cgtcgcgccg
     1501 ctcgggatgg cccctagttg cgccttgcgc aacaccgcgt tcaggtcccc tttgccccca
     1561 atcgtggcca ccgcaaaggt gcccagtcca accaccagga gcaagccgac cgccatcaac
     1621 cctatcaacg accagcgcgg cccccgcttc ttgagccacg gcttggtttg cgtccgctgt
     1681 ttcttgtgca ttacaaatca acctccccgg tttcgttagc aaacgccccg gccatctgca
     1741 cgatcgggtt gctcgccgcc gcggctgcta atgcgaccgc ttcgagattg gcgggctcac
     1801 ccccctgctc gtccgtgcgc tcctgatcgg ccggcagcca aagcgcgcgc ctgccgcccg
     1861 gcgcggcgca ggtttccgcc cattcctcgt cgggataatc ccaggcccgc gcgtcccgat
     1921 ccaggaaatc tcgaaacctc ggcacttggc ggcccttttg cccgtagtag taacgaatac
     1981 ctttccagat ggccaggttc gccacccacg cccgcgattt gagcgggaac agtcgcagcc
     2041 gcctcagtgc cgagcgcggc gaaaagaact cggcgaaggc cgccatttgt gcgtgccgta
     2101 gctccttggc gctcatctgc ttcggttcga aaacgaccga cgcaccattg taacgcagcc
     2161 agtcgcgcgt ccgcagccgc ccctcacgct cgaaccggtc ataaaaagcc gtgcccggat
     2221 agggcgtcaa aatcgggtaa cagctcatgg ccaggttcat gcgctgcgcg aaccggcacg
     2281 cccgcatgca atcctgccaa gtatccgtgt caaagccaaa gataaacgcc ccccacaagc
     2341 tgatgccgaa actctgtatc ttgcggatgc gctcggcgta ggtcgtgctc tgcacgaatt
     2401 tcttgcctgc caccgccagc gtgtcctgct tcgggctttc caaccccaaa atcagcccca
     2461 cgcacccgct ttcacgcatc gtcctcagaa gccggtcatt gcggctgatc aaaatgtcgc
     2521 actgcgtggc ccacgccact ttcagtggcc gcagggcttc gcacagatcg atcgcccagt
     2581 tgtggttccc gccgaagttg tcgtccgcga agatgaagca ccacggccgc cgccgctcac
     2641 aggccgcccg aatctcctcg acgatgtccg caatctcgcg ctggcgatac ttgcgtccga
     2701 agacggcggg cgtcgtacag aagttgcagt tgtgcgggca gccgcgcgtg gtctggatcg
     2761 ggttagcgac aaggtagtcg gcggggttgt agatgtcttt gcgtggcgtg gcgatgggcg
     2821 ccgtctcgaa cgtcgcccag tcataaatgc ggcgcatgcc ctgccgcggg tccatccccc
     2881 ggttggccgc ctcctcccaa tcgcgaatca ggtgcggcaa agttgactcg gcatcgccct
     2941 ggcacaccgc gtcgcaatgt tgcagcgcct cgctcggcat cgcactcggg tgaatcccgc
     3001 ccatgacgac cgtcacgccc agccgccgat acgcgtccgc caactcgtat ccccgcggag
     3061 cttggggcgt catggccgaa atgccgacca ggtccacgtc cgcccgcggt tggtgcggca
     3121 gatcgagcgt acattcgtcg atcagctcga cgtcccagtg ccgtgggacg accgccgcgc
     3181 aaatcgtcag accggtaggc gcgaacaggg ctcggcgatt gaaggtcatc tgccgaatga
     3241 tcttcggcat agtgatgttc gagagcggcg agttcgggtt gatcagacgt atcttgcgcc
     3301 ggcgggcggc ctgggcgaag acggttcctt cccgttgcga agctgtcatc tatccctcac
     3361 gctacgggcg ttccgcgcgg ctgccgcgga cgacgtggcg cttcttgcgc ccatgataca
     3421 gccgcggttc cgatccctta accactgatt ttgaattatc ttcgactggc gatagcttct
     3481 cgcagcggcc gaaccacgtg cggaatcacg cggctggcgg cgtattcctc cccgag
//
LOCUS       DAOSYS010000326         3511 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_21327_length_3511_cov_7.114005, whole genome shotgun sequence.
ACCESSION   DAOSYS010000326 DAOSYS010000000
VERSION     DAOSYS010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21327_length_3511_cov_7.114005"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLU99_14750"
     CDS             <1..243
                     /locus_tag="PLU99_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18352.1"
                     /translation="GVDALWVYDRGCEGPGLPGDLNCDGSVNAFDIDPFVLALTDASG
                     YALAFPGCELMHADINGDGSVNAFDIDPFVSLLTGG"
     gene            complement(385..1449)
                     /locus_tag="PLU99_14755"
     CDS             complement(385..1449)
                     /locus_tag="PLU99_14755"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOO18353.1"
                     /translation="MLLRSKLVVTTAVFLLATGSAWTDEVRVFLVAGQSNAVGCNSDA
                     GELPPELYAPQPDVLFWFEEGPWGTVHDPARRIRSDAWLPLQFQSDPVYGTFGWYDDG
                     FGPEIRLGRTLADHLTADVAVLKFAINATSLAGDWDPDTSGSLYQQMLDILDEALTVL
                     ASQGHTTVLASAFWMQGEWDALYGSYAAAYQSNLTAFITELRADCGNPTLPFVIGRLC
                     AGIEECPYFTFPYLDTVRAAQQAVAASVPNAAMVDTDDLPLNTDFLHFTAEGQLKLGA
                     RFAQAYLAMQFPLGDLNCDGSVNAFDIDPFVLALTSAPGFEAYHAAHPDCDGLLADCN
                     GDGSVNAFDIDPFVELLVGN"
     gene            2009..>3511
                     /locus_tag="PLU99_14760"
     CDS             2009..>3511
                     /locus_tag="PLU99_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18354.1"
                     /translation="MITRAAAWRVTPAVATLLVVAFATVAPVASGAGPMSGAGSTTDA
                     QRTSGAGRTPVLSTAELLVDLARDQGANRRGSQTAADAQQIEVLLKAAVRLNPRLPDA
                     NRWLYELAMLRGDDTAAFEAISRLVAADPGNQSAFARWLDAGLQAGQTIEQRQEWLRS
                     VLSGEGRPAEAQAMVHVQLAKLALAALNRDEARKELEAARALNPADPDAAALRLAMLA
                     PDAAPAERLQVALEYLEVSPLNVDVAWESGVLLDQLGLVEQAEVFLQHAQDVYALANA
                     GAPLPAAHTLQWARHLAARGDLRAASQAIEQMRDADSLLATETAMLYYWLLNKQGREA
                     EASAVRAQLAQNFAALREPSEWPVEMVAQAGWFHCTIDLQAQRALMLAQAAAQRLPND
                     RFVRRVLGWAQAANLMYAEALATLRPIADLDPYAAYQVANILKQQGDVDGAGEVLRNL
                     TALPAGGAARELLDSLGLPQAAMRPASDRLPEVAVVLADFNREVLSFHKDP"
BASE COUNT          575 a         1043 c         1286 g          607 t
ORIGIN      
        1 ggcgttgatg cgctctgggt ttacgaccgc gggtgcgaag gtcccggcct gcccggcgat
       61 ctaaactgcg acgggtcggt caacgcattt gatatcgacc cgttcgtgct ggcgctgacg
      121 gacgcgtccg ggtacgcgtt ggcgttcccc ggctgcgagt tgatgcatgc cgacatcaac
      181 ggcgacggct cggtgaatgc cttcgacatt gacccgttcg tgtcgctctt gacgggcgga
      241 tgagcaaacg ttgacagata gcaggcgcac cggcccgaca cgaagggtgc gccccgctgc
      301 ctaattgcag tcttgctttg ttgcctcggc gccttgcctg gttgctgcga cgcggccggc
      361 caggccgcat tgccggctcg ggcttcagtt gccgaccagc agctccacga acgggtcgat
      421 gtcgaaggca ttcaccgagc cgtcgccgtt acagtcggcc agtaggccgt cgcagtcggg
      481 gtgcgcggcg tgatacgctt cgaatcccgg cgcactggtc agcgctagca cgaacgggtc
      541 gatatcaaat gcgttgaccg acccgtcgca gttcaggtcg cccagcggaa actgcatggc
      601 caggtacgcc tgtgcgaacc gggcgcccag cttgagctgg ccctcggccg tgaagtgcag
      661 gaagtcggta ttcagcggga gatcgtcggt gtcgaccatg gccgcgtttg gaaccgaggc
      721 cgcaaccgcc tgttgcgcgg cgcgcacggt gtccaggtac gggaaagtga agtaggggca
      781 ttcctcgatg cccgcgcaaa ggcggccgat gacgaacggc agcgtggggt tgccgcagtc
      841 cgctcgcagt tcagtgatga aggccgtcag attgctttgg taggccgcgg cgtacgaacc
      901 gtatagcgca tcccactcgc cctgcatcca gaaggcgctc gccagaacgg tcgtgtggcc
      961 ctggcttgca agcacggtta gggcctcatc caggatgtcg agcatttgct ggtagaggga
     1021 gccggaagtg tcggggtccc agtcgcccgc caggctggtg gcgttaatgg cgaacttcag
     1081 gactgcgacg tcggcggtca gatggtcggc cagcgtgcgg cccagcctga tctcgggccc
     1141 gaagccgtcg tcataccagc cgaaggtgcc gtaaaccggg tcgctttgaa actggagcgg
     1201 cagccaggcg tcggaacgga tgcggcgggc cgggtcgtgt acggttcccc aggggccttc
     1261 ctcaaaccag aagagcacgt cgggctgcgg cgcgtagagt tcggggggca gttcgcccgc
     1321 gtcggagttg cagcctacgg cgttagattg gccggcgacc aggaagacgc gcacttcgtc
     1381 cgtccaggcc gaccccgtgg ccagtagaaa cacggcagta gtcacgacga gttttgatcg
     1441 gagcaacatg gctgcaccct ccgtccgccg aggcggacgc tgcttttctt tccgcatcat
     1501 tggtcggcat ggcggaggct acgacctgga ttggctccgc cgacgagccg cgtgggacca
     1561 ttctaccgcg ccgggccgcg ttttgagcgg tcgattttcg gtcgtggcgg ctgccaggcg
     1621 tacgacggac gcgtgcccgc gcgtagcgct tgggcaggca cccgggggcc ggacggcgat
     1681 cagccgcgtt gcctgcccgg ctacgtggcg gttatcctct gccaggaatg gcggttatcc
     1741 tctgccagga atggcggtta tcctgttatt gaattgagca ctggcgttgt aaggaggctc
     1801 agatgaacaa gaaggtcaga agagcgcggg agaaacatcg taagaaccgc gagcggatga
     1861 aacggcgcgt gcgggaacgg cgggcggcgg gcaagcagca ttgagcgcgc cgcgactcgt
     1921 ttttggcgag gtgcgaggag cgcgcgggcg gcgctgggtg tcagcacgga acgcttcaag
     1981 cagtggcacg cggactgcgc tttggcggat gatcacacgc gctgccgctt ggcgggtgac
     2041 gcccgcggtt gccacgctgc tggtcgtcgc gtttgccacg gttgcgccag ttgccagcgg
     2101 tgctggccct atgagcggcg cgggttccac gaccgatgca caacgcacga gcggcgcggg
     2161 gcggacgccg gttttgtcta cggccgaact gctggttgat ctggcgcgcg accagggggc
     2221 gaatcggcgc gggtcgcaga cggcggccga cgcgcagcag attgaagtac tgctcaaggc
     2281 ggccgtgcgg ctcaatccgc ggctgccgga cgctaaccgg tggctgtatg aattggccat
     2341 gctgcgcggg gacgacactg ccgcgttcga ggccatttcc aggctggtgg cggctgatcc
     2401 gggcaaccaa agcgcgtttg cgcgctggct cgacgccggg ttgcaggccg ggcagaccat
     2461 tgaacagcga caggagtggc tgcgaagcgt gctgagtggg gaggggcggc cagccgaagc
     2521 gcaggccatg gtacacgtgc aactggcgaa gttggccctg gcggctctga accgggacga
     2581 ggcccgcaag gagctcgaag ccgcgcgggc gttgaatccc gccgatccgg acgcggccgc
     2641 gctgcgattg gcgatgctcg cgcccgatgc ggcgccggcc gagcggttgc aggtcgcgtt
     2701 ggagtacctg gaggtcagcc cgctcaacgt tgacgtggcc tgggaaagcg gcgtgctgct
     2761 ggatcagctc ggcctggtgg agcaggcgga ggttttcttg cagcatgcgc aggacgttta
     2821 cgcgctggcc aacgcggggg cgcccttgcc ggcggctcac acgctgcaat gggcacggca
     2881 cctggcggcg cgcggcgacc tgcgggctgc cagtcaagcg attgagcaga tgcgcgacgc
     2941 tgactcactg ctggccaccg aaaccgcgat gctgtactac tggctgctca acaagcaggg
     3001 ccgggaggcg gaggccagcg cggtgagggc gcagttggcg cagaacttcg cggcgctgcg
     3061 cgagccgagc gagtggccgg ttgagatggt cgctcaggcc ggctggttcc attgcacgat
     3121 cgacctccag gcgcagcggg cgttaatgct ggcgcaggcg gcggcgcagc ggctgcccaa
     3181 cgatcgattc gtgcgccggg ttctgggttg ggcacaggcc gcgaatctga tgtatgcgga
     3241 agcgctggcg acgctgcggc cgatcgccga cctggatcct tatgcggcct accaggtggc
     3301 caacatatta aagcagcagg gggatgtgga cggtgccggc gaggtgctgc gcaacctgac
     3361 agccctgccg gcgggcggtg cggcgcgcga attactggat agtctgggtt tgccgcaggc
     3421 cgcgatgcga ccggcctcgg accgcctgcc ggaagttgcc gtggttctgg ccgactttaa
     3481 tcgcgaggtt ttgagttttc acaaagatcc c
//
LOCUS       DAOSYS010000327         3484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_21509_length_3484_cov_5.811024, whole genome shotgun sequence.
ACCESSION   DAOSYS010000327 DAOSYS010000000
VERSION     DAOSYS010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21509_length_3484_cov_5.811024"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3220
                     /locus_tag="PLU99_14765"
     CDS             <1..3220
                     /locus_tag="PLU99_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOO18355.1"
                     /translation="NSLIIFFSLKNNYALKELVVAFDTLPVGADTEVRAIALKYAGAK
                     QVSEVLEKVFKEGKKALARPTEGDASPAKGEMPPVPPTKTGEGLPYNLIVQSDERSNT
                     VIVVGRKDAVLLAAGLIAELDRPSAEFVGQPFVIQLKRYPASQLEEKLSQMLEDRAKA
                     VGGDQNEARDSAILMADDRSNSLVVLASREMFDMIEDMATQLDALDGYSVVDMRYRRL
                     EYADAEKLANMLQELFKRKADAQGKQAKDFDASLEALADVRSNGLLLVGTRDFLAEAE
                     TLIDNLDQKYDPTVVFHIRPVKLNSAVNIAGLLKDMVDKALKEQDSKLKGTPIYVAAD
                     PISDSLLLAASQEDMDRLEGWVNWLDRPSELGRMIRIIPMRHGSADKVSKTVGDLYKN
                     LSGSSSGGGKIDVSVTFDATTNSVVAFAPPAILAEIENSVRALDQTDPAGGTLVRIFN
                     LEQADAQSAGDLLTSILSLRGGSVGGSGGGRGGTSGGASQEQIAKQVMLLFQRENPDL
                     GIQTLKALRGDVSVVSDLRTNSLVITAPAEGMALMEALVQAIDVPPNQAKVRVFPLRN
                     ADAEQMVNTLTDLFQQKTGGSSGGSRSGSQSQETLALSLGESVGGQEQISFTTDMRTN
                     SVIAAGTRGYLDLVEQLVLDLDSRPVVERKTRVYAPRNMVAKDLADAIKSYSDSERDR
                     LQELGDDISLAVKQEAEIGAIPYEQSNRIILAYSPRKEEEVLDIVRQLDQQPAQVSIE
                     VLIVEVACSNSLELGVEWAFQDLQYAKAGPADTTTFDFVGGTDVGAAGAGLGGFTFTI
                     TGRDFNFLLHALQSENSLNVLSRPHIVAMDNQEAKIEITDSIPYVTGSATTIGGSLQT
                     SVSRQDVGIKLEVTPQINPDGFVRMEIRQEVSDLSDSTISVGPGVTAPIFNQRIAETV
                     VTVQDNETVVLGGLIQSRDLKSEQKVPLLGDIPVLGPLFRFQSDDTRRAELLVILTPR
                     VIRTREDFHEMSVEARDQNCMLPEDIKCNPLMRGLRVRPEDLAPCGEGGLLGPFPNDA
                     SQTPVLNGPSPEEYGPVRPESAPPNDLDTYNVPTSMLMKRRS"
     gene            3221..>3484
                     /locus_tag="PLU99_14770"
     CDS             3221..>3484
                     /locus_tag="PLU99_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18356.1"
                     /translation="MTPKQRLFFSSVASFLLLLGASACETPQPGAKPLFADLFGPQPP
                     PAKPSRGSASSTKAAPDVDVTLVPTRDDITAVYQFFAQDPWLRN"
BASE COUNT          696 a         1188 c         1041 g          559 t
ORIGIN      
        1 caactcactg atcatcttct tctcgctcaa gaacaactac gccctcaagg agctcgtcgt
       61 cgccttcgac accctgcccg tcggcgctga caccgaggtg cgcgctatcg ccctcaagta
      121 cgccggggcc aagcaggtca gcgaggttct cgaaaaggtc ttcaaggaag gcaagaaggc
      181 cctggcccgc ccgaccgaag gcgacgccag cccggccaag ggcgaaatgc cgcccgttcc
      241 gcccaccaag accggcgaag gcctgcctta caacctcatc gtgcagtccg acgagcgcag
      301 taacaccgtc atcgtcgtcg gccgcaagga cgccgtgttg ctggcggccg ggctcatcgc
      361 cgagctcgat cgcccctcgg ccgaattcgt cggccagccg ttcgtcattc agctcaagcg
      421 ctaccccgcc tcccagctcg aagaaaagct cagccagatg ctggaggacc gggccaaggc
      481 cgtgggcggc gaccagaacg aggcccgcga cagcgccatc ctcatggccg atgaccgctc
      541 aaactccctg gtcgtgctcg cctcacgcga aatgttcgac atgatcgaag acatggccac
      601 ccagctcgac gcgctcgacg gctacagcgt ggtcgacatg cgctatcgcc ggctggaata
      661 cgccgacgcg gaaaagctgg ccaacatgct gcaggaactc ttcaagcgca aggccgatgc
      721 ccaaggcaag caggccaagg acttcgatgc ctccctcgaa gccttggccg acgtgcgcag
      781 caacggcctg ctcctggtcg gcacgcgtga cttcctggcc gaagccgaaa ccctgatcga
      841 caacctggac cagaagtacg acccgaccgt ggtattccac attcggccgg taaagctgaa
      901 cagcgccgtc aacatcgccg gcctgctgaa ggacatggtc gacaaggccc tcaaagagca
      961 ggattccaag ctcaagggca cgcccatcta cgtcgccgcc gatcccatct ctgacagcct
     1021 gctgctggcc gcctcgcaag aggacatgga ccggctggag ggctgggtga actggctcga
     1081 tcgtcctagc gaactggggc gcatgatccg cattatcccc atgcggcacg gttcggccga
     1141 caaagtctcc aagaccgtgg gcgatctgta caagaacctc tccggcagct cgagcggtgg
     1201 cggcaagatc gacgtgagcg tcacgttcga cgcgaccacc aactcggtcg tggccttcgc
     1261 gccgcccgcc atcttggcgg aaatcgaaaa ctccgtgcgc gccctggatc agaccgatcc
     1321 ggccggcggc accctggtgc gcatcttcaa tctcgagcag gctgacgccc agtcggccgg
     1381 cgacctgctg accagcattc tctcgctgcg cggcggcagc gtcggcggca gcggtggcgg
     1441 gcgtggcggc accagcggcg gcgccagcca ggagcagatc gccaaacagg taatgctgct
     1501 cttccagcgc gaaaacccgg atctgggaat ccagacgctc aaagccctgc gcggcgacgt
     1561 cagcgtggtt tcggatctgc gcacgaactc actcgtgatc accgcgccgg ccgagggcat
     1621 ggccttgatg gaagccttgg tgcaagccat cgacgttccg cccaaccagg ccaaggtgcg
     1681 cgtcttcccg ctgcgcaacg ccgatgctga gcaaatggtc aatacgctta ctgacctctt
     1741 ccagcaaaag accggcggca gtagcggcgg cagccgaagc ggcagtcaaa gtcaggaaac
     1801 gctggccctc tcgctgggcg aaagcgtggg cggccaggaa caaatcagct tcaccactga
     1861 catgcgcacg aattccgtaa tcgccgcggg tacgcgcggc tatctggacc tcgtggaaca
     1921 gcttgttctg gacctcgaca gccgcccggt ggtggagcgc aagacccgcg tctacgcccc
     1981 gcgcaacatg gtggccaagg atttggccga cgccattaag agctacagcg acagcgagcg
     2041 ggaccggctt caggagttgg gtgacgacat ctcgctcgcg gtcaagcaag aggccgaaat
     2101 cggggccatc ccctacgagc aatcgaaccg catcatcctc gcctacagcc cgcgcaagga
     2161 agaggaagtg ctggacatcg tccggcagct cgaccagcag ccggcccagg tgagcatcga
     2221 agttctcatc gtcgaagtcg cctgcagcaa ctcgcttgag ctgggcgtcg agtgggcctt
     2281 ccaggatctg caatacgcca aagccggtcc ggcggatacc actaccttcg acttcgtcgg
     2341 cggcaccgac gtcggggcgg ccggcgccgg cctgggcggc ttcaccttca ctatcaccgg
     2401 ccgcgacttc aacttcctcc tgcacgcgtt gcagagcgag aacagcctca acgtgctcag
     2461 ccggccgcac atcgtggcca tggataacca ggaagccaag atcgagatca cggacagcat
     2521 tccctacgtg accggctcgg ccaccacgat tggcggatcg ctgcagacct cggtctcgcg
     2581 gcaggacgtg ggcatcaagc tcgaagtcac gccgcagatc aatccggacg gcttcgtgcg
     2641 catggagatc cgccaggaag tttcggattt gagcgactcg acgatttccg tcggtccagg
     2701 cgtaaccgcg ccgattttca atcagcgcat cgccgagacg gtggtgaccg tgcaggataa
     2761 cgaaacggtt gtgctgggcg ggttgattca gtcgcgcgat ctcaagagcg agcagaaggt
     2821 gccgctgctg ggcgacattc ccgtgctggg gccgctcttc cgcttccaga gcgacgacac
     2881 ccgccgcgcc gaactgctcg tgatcctcac gccgcgcgtc atccgcaccc gcgaggattt
     2941 ccacgaaatg tctgtcgagg cccgcgatca gaactgcatg ctgcccgaag acataaagtg
     3001 caatccgctg atgcgcggcc tgcgcgtgcg cccggaagat ctggcgccgt gcggcgaagg
     3061 cggcctgctc ggcccattcc ccaatgatgc ctcccagacg ccggtgctga acggcccctc
     3121 gcccgaggaa tacggcccgg tccgcccgga atccgcgccg ccgaacgacc tcgataccta
     3181 taacgtgccg accagtatgt tgatgaaacg acggagttag atgaccccga agcaacgcct
     3241 cttcttctcg tcggtagcgt ctttcctgct tctgctgggc gccagcgcct gcgagacgcc
     3301 tcaaccaggc gccaagcccc tatttgctga cttgtttggc ccgcaaccgc cgcccgccaa
     3361 gccatcccgg gggagcgcct caagcactaa ggccgctccc gacgtggacg tcaccctcgt
     3421 acctacccgc gacgacataa cggccgtcta ccagttcttc gcgcaggatc cctggctgcg
     3481 gaat
//
LOCUS       DAOSYS010000328         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_21737_length_3450_cov_5.780265, whole genome shotgun sequence.
ACCESSION   DAOSYS010000328 DAOSYS010000000
VERSION     DAOSYS010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21737_length_3450_cov_5.780265"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLU99_14775"
     CDS             complement(<1..1575)
                     /locus_tag="PLU99_14775"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOO18357.1"
                     /translation="MHRWLGGCLVLIIANLALAQTGPLVPQELLDQLQASEPQGLPRN
                     MTPQEALLPLPTAERGSRTPPSGTVYCPPEYVRNEGIFMNWKQYTSLITQMIVPITTQ
                     DPEGIVHLVVDNASAQSSAAATLAAAGADLSRVNFIVYQTNAVWIRDYGPRYILEDGA
                     QTIIDHVYNRPRPLDDGFNDFLADYWDAPQYDIGLVHGGGNFHLFANGDAFMTTLVLS
                     ENPSLTAEDVIQRYHDYQNLNLTLYQGFPFSFDGTAHIDMWMLPVDDWKVIIGQYEPS
                     DGSPYTITEGAVADLTARGYTVYRTPGWNSGGTHYTYTNAVIFNDLVFMSRFNVPQDN
                     VALAVFQEAFPGKTIYQLDSSSIIQAAGAMHCITMHMPAAAGLYIGLAAAAPEFIAPQ
                     TPTPITVRIENRGETYVPGSGLLYYRFDGGAWLTAPLASLGDNVYEGLLPGAGCGDAP
                     EFYFSATGDQGTVVYLPSDAPAQVYTATVATVTTLLSDDFESDLSWTVDSAPGMTSGM
                     WTRAVPPADDYGPDRDY"
     gene            1891..>3450
                     /locus_tag="PLU99_14780"
     CDS             1891..>3450
                     /locus_tag="PLU99_14780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18358.1"
                     /translation="MNMETLAASGGKAATRSPAKPANLAGATNPRGGCGALRLGLLIA
                     AVGIATAAAHWPALSAQARTFDDRMYLINNPLVQNPSWASVGRFFREILRPSTVQGYY
                     QPLAMTSLMLDYARGGSSHDLRAFHETSLALHVLNTGLILVLLHLLFRQPVVAAAIAL
                     LFGLHPLTVEPIPWVSERKTLLATCFVLCCLILYVQWARRRRRLYFLGAGIAFVLALL
                     SKPTSTPLPILLLLLDYWPLRRMGKRALLEKIPLLAIAGAAAVITVVSQGQTAYLELP
                     HLQSGARIPLTICHNVVFYLSKMVWPGSLTPHYPIPNPFDLSHPALLAGVVGTVVLVA
                     LLLVSWRWTRALLTGWLWFFVAIFPTLGVIGFTNVITSDKYAYLPAVGILLILAFALT
                     RVWGDPALAARLRWRRLLVLACVAIIALLEARATRRQLAYWENTEKLYRYMLTLAPQD
                     VFLHSELGSELSDQGRYEEAVAHFETALRLYPNSYVDHNNLATVLMAMGRFGEAVAHF
                     EAALRIKPDITA"
BASE COUNT          580 a         1033 c         1141 g          696 t
ORIGIN      
        1 gtaatccctg tccgggccgt aatcatcggc gggcgggacg gcgcgcgtcc acatgccgga
       61 cgtcatgccc ggcgcggagt ccacggtcca actcaggtcg ctttcgaagt cgtcgctgag
      121 caaggtcgta acggtcgcga cggttgcggt gtagacttgc gccggggcgt cactgggcaa
      181 gtacacaact gtgccctggt cgccggtggc gctgaaatag aactccggag catcgccaca
      241 gccggccccc ggcaacaggc cctcgtacac gttgtcgccc agggacgcca gaggagcggt
      301 cagccaggcg ccgccatcga aacggtagta gagcaacccc gagcccggta cgtaagtctc
      361 gccacgattc tcgatgcgca cggtaatcgg cgtgggcgtc tgcggggcga tgaattccgg
      421 ggccgcagcc gccaggccta tgtacaggcc ggcggcggcg ggcatgtgca tggtaatgca
      481 gtgcatggcg cctgcggcct gaataatgct ggagctgtcg agctggtaga tagtcttgcc
      541 ggggaaggcc tcctggaaca ccgccaaagc aacgttatcc tggggcacgt tgaaacgcga
      601 catgaagacc aggtcgttga atatgacggc attcgtatag gtgtagtgcg tgccgcccga
      661 gttccagccg ggagtgcgat agaccgtgta gccgcgggcc gtcaggtcgg ccacggcgcc
      721 ctcggtaatc gtgtacggcg agccgtcaga cggttcgtat tggccgatga ttactttcca
      781 gtcgtcgacc ggcagcatcc acatatcaat gtgcgccgtg ccgtcgaagc tgaacgggaa
      841 gccctggtaa agcgtcaggt tgagattctg gtagtcatgg tagcgctgga tcacgtcctc
      901 ggcggtcaag ctcgggtttt cagacaaaac gagggtagtc atgaaggcgt cgccgttggc
      961 gaacaggtgg aagttgccgc cgccgtgaac caggccgatg tcgtactgcg gcgcgtccca
     1021 gtaatcggcc aggaagtcgt tgaagccgtc atccagagga cgcgggcgat tgtagacgtg
     1081 gtcaatgatc gtctgcgcgc cgtcctccag aatgtaacgc ggcccgtaat cgcgtatcca
     1141 tacggcgttc gtctggtaaa cgatgaagtt tacccggctc aggtcagccc ccgcggcagc
     1201 cagcgtggcg gccgccgagg actgtgctga ggcgttgtcc accaccaggt gcacgatacc
     1261 ctcggggtct tgcgtagtga tggggacaat catctgggtt atcagggagg tgtactgttt
     1321 ccagttcatg aagatgccct cgttacggac atactccggc ggacagtaca ctgtgccgct
     1381 tggtggcgtg cggctgccac gctcggccgt gggcagcggc aacagcgctt cctgaggcgt
     1441 catgttccgc ggcagaccct gcggctcact ggcctgaagc tgatccagga gctcttgcgg
     1501 aaccagcggg cctgtttgag ccagcgccaa gttcgcaata atcaagacga gacagccacc
     1561 taaccagcga tgcattctcc tcactccttt cgcgctccag cggcgcgctc gccatatggc
     1621 gggttggggc cggaagtgag ctccctatcc actccagatc gcgagagcgt gtgctccggc
     1681 cgatcagccg taacatgcat tgtatgaaat ttcgctcgcg ggcctaatac gtttttcggt
     1741 tttgtttgaa gcggcgtggc gggcggttca gccgttaacg tccgatatcg cgtcgccggc
     1801 cgccgccgcg tgtcagtcac tgcgcgaggc cttataatgc caccgcgtaa gccggcgccc
     1861 gactctggcg aggtcgtctg ggaatgacgg atgaatatgg agacgctcgc ggccagcggc
     1921 ggcaaagcgg ctacgcgtag ccctgcgaag cccgccaacc ttgcaggcgc gactaatcca
     1981 cggggcggtt gcggcgcgct gcgcctgggg ttgctgatcg cggccgttgg cattgcgacc
     2041 gcggcggcgc actggcccgc gctgtcggct caggcgcgca ccttcgacga ccgcatgtac
     2101 ttgatcaaca acccgctcgt gcagaacccg agctgggcgt ctgtcgggcg cttcttccgc
     2161 gaaattctgc ggccgtcgac cgtgcagggt tactaccagc cgctggcgat gacgtcgctg
     2221 atgttggact acgcgcgcgg cggcagctcg cacgatctgc gggcgtttca tgagaccagc
     2281 ctggcgctgc acgtgctgaa caccgggctg atcctggtgc tgcttcacct gctgtttcgg
     2341 cagccggtcg tggcggcggc catagccctg ctgtttgggc tgcacccgct cacggtcgag
     2401 ccgattccct gggtcagcga gcgtaagacg ctgttggcta cgtgctttgt tctgtgctgc
     2461 ctgattctgt acgttcagtg ggctcggcgg cggcgccggc tgtatttcct cggagcgggg
     2521 atagcgttcg ttctggcgct gctgtccaag ccgaccagca cgccgctgcc gatcctcctg
     2581 ctgctgctgg attattggcc gcttcgtcga atgggcaaac gcgccctgct ggaaaaaatc
     2641 ccgcttctgg cgatcgcggg cgcggcggcg gtcataactg tagtctcgca gggacagacg
     2701 gcctatctgg aactgccgca cctgcagtcc ggcgcgcgga ttccgctgac gatctgccac
     2761 aatgtggtgt tctatttgtc caagatggtc tggccgggat cgctgacgcc gcactatccg
     2821 atacccaatc cgtttgacct ttctcatccg gcgctgctgg ccggcgtggt gggcacagtc
     2881 gtgctggtcg cgttgttgct ggtgtcgtgg cgctggacgc gggcgttgct caccggctgg
     2941 ctgtggttct ttgtggcgat tttcccaaca ctgggcgtga tcgggtttac aaacgtaatt
     3001 acgtccgaca agtacgccta cctgccggca gtgggaatat tgctgattct ggcgttcgca
     3061 ctgacgcgcg tctggggcga tccggcgcta gcggcgcgcc tgcggtggcg ccgtctgctc
     3121 gtgctggcgt gtgtggcgat tatcgcgctg ctcgaggctc gcgccacgcg ccgccagctc
     3181 gcctactggg agaatacgga gaagctatac cggtacatgc tgacgctcgc gccgcaggat
     3241 gtttttctgc actccgaact gggaagcgaa ctcagcgatc aggggcggta tgaggaggcc
     3301 gtggcccatt ttgaaactgc gctgcgcctg tacccgaatt cgtatgtgga tcacaacaac
     3361 ctggcgacgg tgctgatggc catgggacgg ttcggcgagg ccgtggcgca cttcgaagcg
     3421 gccttgcgga tcaagcccga cataaccgcc
//
LOCUS       DAOSYS010000329         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_22063_length_3404_cov_4.762019, whole genome shotgun sequence.
ACCESSION   DAOSYS010000329 DAOSYS010000000
VERSION     DAOSYS010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22063_length_3404_cov_4.762019"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1871)
                     /locus_tag="PLU99_14785"
     CDS             complement(159..1871)
                     /locus_tag="PLU99_14785"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOO18359.1"
                     /translation="MRSLVVLGLALVIRVSVPAQAEMNLDQELRADVVLRALVDELQR
                     DHSGLALEGLERPYFIEYALADASRASVSATLGAVTSHNEYRNRSLRTDVRVGSYELD
                     NTNFRSGGYSFFGRAGSSSGAAMPIQDDYHAIRQAIWWATDRDYKAVVEALVQKKAIM
                     ESKLIEDKPPDFSREQPVVNFDERAPVELDLAAMEAIATVVSAVFCDYPEIQNSNVTL
                     SAGSGNRYLVNTEGTRLRFAGLRCVLSVSATVQADDGMKLSDSFLIFGNTPDDLPPPE
                     QVIQRCHDMAARLLALKNAPVLTSYSGPVLFEAEAAARIFARQFGYSFAGGQRAVGSR
                     AGPDDFANKLNRRILPRFMNVVDDPTLEEIEGVPVLGHYEYDDQGVPAQPVTLVENGR
                     LRALLMSRNPSREFTQSNGHGRGFMGQPRAAVGCLVVTADEGLDAATLRQELLEAAAD
                     EGLECALRIASLGGVGDSAGRFGGEGFSPLVMYKVYPDGREELVRGAEIARIDLKAFK
                     RILAAGDTPYVYNTPASEGQTYAVPALLFEELDLAKVDRDYDKPPILPSPLARKDQEV
                     TARP"
     gene            complement(1921..>3404)
                     /locus_tag="PLU99_14790"
     CDS             complement(1921..>3404)
                     /locus_tag="PLU99_14790"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOO18360.1"
                     /translation="NHSRSLDVDVRCGDYALDNTRQIRGSEAGFDFGHSATSLPLNGD
                     SLATRHAIWLATDAKFKAAVKRLAQVKANLKVKVEEEDPADDFSREAPNTHIGPWVTH
                     TADREALAARAQRLSQRFREFPLIYSSSVTISGGATNNLHVNSEGSRLQFGRTAWRIG
                     IQAGTIAEDGMELWQYRAFDSATLEGLPDDDEVMKAVDQVIAEILALREAPLVEPYTG
                     PAILLNRASGVFFHEIFGHRLEGHRQKDVEEGQTFAKKIGEPVLPEFLSVLDDATLRR
                     FKGVDLNGFYEFDDECVPAQQVKLVTNGVLKSFLLSRSPTRGFTQSNGHGRRESGATV
                     VARMGNLIIESQHQVPFDELRQMLISECKRQGKEYGLLFTDISGGYTITERYMPQAFK
                     VVPILVYRVYTDGRPDELVRGVDIVGTPLTCFSKILCTADDPAVFNGYCGAESGSVPV
                     SAISPSILVEQIEVEKKQKAQDRPPILQAPIAEEKGALGAAQN"
BASE COUNT          594 a         1051 c         1089 g          670 t
ORIGIN      
        1 tcagccagag gcgcttgcca gcgtttgcgc ggcagtggcc cagagcagag ccggggcgtg
       61 ccgccccgaa atgccgccaa gcgttccccc tccgcgcagc accagctccg actacaccat
      121 gtcgcgccgt ccttgggcat gccgcccagg ctgcattgct atgggcgcgc ggtgacctct
      181 tggtccttgc gagccagggg gctcggaagg atcggcggtt tgtcgtagtc gcgatccact
      241 tttgccaggt cgagttcctc gaacagcagc gcggggacgg cgtacgtctg gccttcactg
      301 gcgggcgtgt tgtacacata gggcgtatcg cctgccgcca gtatgcgctt gaatgccttg
      361 agatcgatgc gggcgatctc ggccccacgc acgagttcct cgcggccgtc ggggtacacc
      421 ttgtacataa ccagcgggct gaagccctcg ccgccgaaac gcccggcgct atctccgacg
      481 ccgcccagac tggcgatgcg cagggcgcat tcgagtcctt cgtccgcggc cgcctccagc
      541 agctcctggc gcagcgttgc cgcgtcgaga ccctcgtcgg ccgtaaccac caggcagccg
      601 acggccgcgc gcggctggcc catgaagccg cgtccgtggc cgttggattg ggtgaattcg
      661 cgggaaggat tgcgtgacat cagcaaggcc cgcagccggc cgttttcaac taacgtcacg
      721 ggctgcgccg gcacgccctg gtcgtcgtac tcgtagtggc cgagcaccgg aacgccttcg
      781 atttcctcga gcgtcgggtc atccacgacg ttcatgaagc gcggcaagat gcggcggttg
      841 agcttgttgg cgaaatcgtc cgggcccgcg cggctgccga ccgcgcgctg tccgccggcg
      901 aacgaatatc cgaactgccg ggcgaagatt cgggccgccg cctcggcttc gaacagcacc
      961 gggccggaat aggaggtcag caccggcgcg ttcttcagcg ccagcaaccg ggcggccatg
     1021 tcgtggcagc gctggatgac ctgttcgggc ggaggcaggt cgtcgggcgt gttcccaaag
     1081 atcaggaatg aatcggacag tttcatgccg tcgtcggcct ggacagtggc gctgaccgac
     1141 agcacgcagc gcaatccggc gaaacgcagc cgcgtgcctt cggtattcac caggtaacga
     1201 ttgccgctgc cggcgctaag ggtgacgttg gaattctgga tttcaggata gtcgcagaag
     1261 acagcggaga cgactgtggc gatggcctcc atcgcggcca aatcgagctc taccggcgcc
     1321 cgctcgtcga agttgacgac cggctgctca cgtgaaaagt cgggcggctt gtcctcgatg
     1381 agcttgcttt ccatgatggc cttcttctgg accaaggcct cgaccacggc tttgtagtcg
     1441 cggtcggtgg cccaccagat ggcctggcga atggcgtggt agtcgtcttg gatcggcatg
     1501 gcggcaccgc tgctgctgcc cgccctcccg aaaaaactgt agccgccgct gcggaaattt
     1561 gtgttgtcca actcgtacga acccacgcgc acgtcggtcc gcagcgaacg gttgcgatac
     1621 tcgttgtgcg aagtgaccgc gcccagcgtg gcggataccg aagcgcgact cgcgtcggcc
     1681 agtgcatact caatgaagta cggccgctcg agcccttcga gggccaagcc ggaatgatcg
     1741 cgctgcagtt catccaccag tgcccgcagc acgacatcgg cgcgcagttc ctgatcgagg
     1801 ttcatttcgg cttgcgccgg cactgaaacg cgaatcacca gcgccagccc aagcacaacc
     1861 agggatctca tgggttgctt gctaataggg agtgttttca tcggtccttt ccggagagct
     1921 ttagttctgg gcggcgccga gtgcaccttt ttcctcggcg atgggagctt ggagaatggg
     1981 cgggcggtcc tgagccttct gtttcttttc gacctctatc tgctcgacca ggatgctggg
     2041 tgagatggcg gaaaccggca cggagccgga ttccgcgccg caatagccgt taaacaccgc
     2101 cggatcgtcg gcggtgcaga gtatctttga gaaacaggtc agcggcgttc cgacgatatc
     2161 gacgccgcgc acgagttcat cggggcgtcc gtcggtgtac acgcggtaga cgagtatggg
     2221 caccaccttg aacgcttgcg gcatgtagcg ctcggtgatc gtgtagccgc cggaaatatc
     2281 agtgaacagc aggccgtatt cttttccttg ccgtttgcac tcgctgatga gcatctgacg
     2341 cagctcgtca aacgggacct ggtgctgcga ttcgatgatc agatttccca tgcgcgccac
     2401 gacggtggca cccgactcgc gccgcccgtg cccgttggat tgcgtgaagc cgcgcgtggg
     2461 gctgcgcgag agcagaaagg acttaagtac gccgttagtg accagcttca cttgttgagc
     2521 cggcacgcac tcgtcgtcga actcgtaaaa gccgttaaga tccacgcctt tgaaccggcg
     2581 cagcgtggca tcatccagga cacttaggaa ctccggcagg acgggctccc cgattttttt
     2641 tgcgaaagtt tgcccttctt cgacgtcctt ctggcgatgc ccttcgagac gatggccgaa
     2701 aatttcatga aagaagacgc cgctggcgcg gtttagcaga atcgcgggcc cggtgtatgg
     2761 ctcgaccagc ggggcctcgc gcagggcgag aatctcggcg atcacctgat cgaccgcctt
     2821 catcacctca tcgtcgtccg gcaggccttc cagagttgcg gagtcgaatg ctctgtattg
     2881 ccaaagctcc atgccatctt cggcgatcgt gccggcctgg atgccgatgc gccacgcggt
     2941 ccgcccgaat tgcaggcggc tgccttcaga attgacatgc aaattgttgg ttgcaccgcc
     3001 ggagattgtg actgaagaac tatagatcag cgggaattcc cggaagcgct gtgacaggcg
     3061 ctgcgcccga gctgccagcg cctcccgatc cgcagtatgc gtcacccacg gaccgatgtg
     3121 cgtgttgggc gcttcacgcg agaagtcgtc ggccggatct tcctcttcca ctttcacctt
     3181 gaggttggct ttgacttgcg ccaggcgctt cacggcggct ttgaacttgg cgtccgtggc
     3241 tagccaaatg gcgtggcgtg tcgccagcga atcgccgttg agcgggagcg atgtcgcgct
     3301 gtggccgaaa tcaaatccgg cttcggagcc ccggatttgg cgagtgttgt cgagggcgta
     3361 gtcgccgcat cggacgtcga cgtccagcga gcgcgagtgg ttag
//
LOCUS       DAOSYS010000330         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_22168_length_3392_cov_3.542403, whole genome shotgun sequence.
ACCESSION   DAOSYS010000330 DAOSYS010000000
VERSION     DAOSYS010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22168_length_3392_cov_3.542403"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLU99_14795"
     CDS             complement(<1..848)
                     /locus_tag="PLU99_14795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HOO18361.1"
                     /translation="MSKQSADPVPAETGVLSAPPPGELFGHPKGLFVLFSTELWERFS
                     FYSMRGILTLYMVNVLAAAGGHADQMYGAYLGFVYAATFGGGMLADRLLGQRRAIYIG
                     GVLMALAQFTLATHSLLVRSGYSVRGLDPMFFSGLGLLACGNGFFKPNISTVVGTLYK
                     PGDQRRDSAFTIFYMGINIGAFLAGFSGQIAETWGWYLGFLLAGMGMVASLVVFTAGL
                     RQLSGKGLPPEGATLTKGRFGIPNAVLVVIGVLLFVPAAAFLISKPSYVQSMAVVIAL
                     IVLPYLL"
     gene            1051..>2575
                     /locus_tag="PLU99_14800"
     CDS             1051..>2575
                     /locus_tag="PLU99_14800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOO18362.1"
                     /translation="MSVSSTAAPAKMSVAQQIGSLPFNYWVANAMEMLERLAFFSLLA
                     IRPLFAKAPASENGLELSYAEVGTVFGIWAWLQCIIPMISGGYTDRYGYRKSLIVAFI
                     LNGCGYLLLARALPVAEWLTTLDVPNASFWVLLGAACLIGPGTAIFKPAVQGTLARVV
                     NAETSSMAWGLFYWVVNVGGFLAPILAAWLRGEAGLNWSNVFYGCAIVTALNFIPALF
                     IYREPERVVPAEGQKGPLGVFVTSLAMLLRDVRFVLFLLIAACFWFMFMQLWDLLPNF
                     LNEWVDTSAVGRVLKSIFGENLTWVLPNGQVKPEMIINIDATSILLFVLVISWMIRRM
                     HKIAAMILGMLISLVGFVGAGATNVGAICCLMIFVFAIGEMICSPTFSAYIGLIAPPA
                     RKALYMGYSNIPFAIGWGMGNFVSGPMYDQIGSKEMLARGHLVQVYQLPAELATKDNK
                     AFPSDQVVPSLAYVTKTGNVENLREIIASFAGQEKPDAEALKQAYAPIREQVSPADLD
                     "
     assembly_gap    2576..2585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2756..>3392
                     /locus_tag="PLU99_14805"
     CDS             2756..>3392
                     /locus_tag="PLU99_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOO18363.1"
                     /translation="MNNPSTNAVQPSSIELPPEADAAVAIVRKLVDAGHTALLAGGCV
                     RDLLLGHPPQDYDVATSARPEQIVQLFRSTRLVGAQFGVVLVRKRRRWVEVATFRWDG
                     PYLDGRRPSKVVFSDAREDALRRDFTVNGMFFDPLAGQVIDHVGGQPDLRAGVIRAIG
                     EPSARFEEDHLRLLRAVRFAARLNFPIEPQTFAALREHAPQLAKVAVERVRD"
BASE COUNT          614 a         1031 c         1118 g          619 t
ORIGIN      
        1 aggaggtacg gcagcacaat cagggcaatc actaccgcca tcgactggac gtagctcggt
       61 ttcgagatca ggaatgcggc ggcgggcacg aacagcagca cgccgatcac cacgagtacc
      121 gcattgggta tgccgaaacg tcccttggtc aacgtggcgc cttcgggcgg cagtcccttt
      181 ccggaaagct gtcgcaggcc ggccgtgaac acgacgagcg acgcgaccat tcccatgccg
      241 gccagcagga acccgagata ccagccccag gtctcggcga tctgacctga gaaaccggcc
      301 agaaaagcgc cgatattgat gcccatgtag aagatcgtaa aggcgctgtc acgccgctgg
      361 tcgcccggct tatacaaggt gccgacgacg gtggaaatat tcggtttgaa gaagccgttg
      421 ccgcatgcga gcaggcccaa accggagaaa aacatcggat cgagcccgcg caccgaatac
      481 ccggaacgaa ccagcaacga gtgagtggcc aacgtgaact gcgccagggc catcagtacg
      541 ccgccgatat agatcgcgcg gcgctggccc agcaggcggt cggccagcat gccgccgcca
      601 aacgtggcgg cgtacacgaa cccgagataa gcaccgtaca tctggtccgc atggccaccg
      661 gcggccgcga gcacgttgac catgtaaagg gtcagaatgc cgcgcatgct gtagaagctg
      721 aagcgctccc acagctcggt gctgaagaga acaaataggc ctttggggtg gccgaatagc
      781 tcgccaggcg ggggagctga caggacaccg gtctcagcgg ggaccggatc ggcagactgc
      841 tttgacacgt acgactccga ttgcccgccg cggcgggcgt aaagctttcg ccggctgcgc
      901 acagcaaacc cgcccggcgg cgccgccggc ggggtgcggc tagcatacgg caagccgcgg
      961 cggcactcca gcggaagttc ttatggttgc acgacttccc gccgggtgct gcggcggata
     1021 taaacgctcg gtctgacacc tggagacgtt atgtccgtga gctcgaccgc cgcgccggcg
     1081 aagatgagtg tggcccagca gatcggcagc ctgccgttca actactgggt ggccaatgcc
     1141 atggaaatgc tggagcggct ggccttcttc agcctgctcg cgattcgccc gctgttcgcc
     1201 aaggcgcctg ccagcgagaa cggcctcgaa ctgagctatg ctgaggttgg caccgtcttc
     1261 ggcatctggg cgtggttgca gtgcatcatc ccgatgatct ccggcggtta cacggaccgg
     1321 tacgggtacc gcaaaagcct gatcgtggcg ttcatcctga acggctgcgg ctacctcctg
     1381 ctggccagag cgctgcccgt ggcggaatgg cttaccactc ttgatgtgcc caacgcgagc
     1441 ttctgggtac ttttgggcgc ggcctgcctg atcggtccgg ggacggccat tttcaaaccg
     1501 gcggtgcagg gcacgctggc gcgcgtggtg aatgccgaaa cctcgtcgat ggcctggggc
     1561 ttgttctact gggtggtcaa cgtgggcggg ttcctggccc cgatactggc ggcgtggctg
     1621 cgcggcgagg ccgggctgaa ctggagcaac gtgttctatg gctgcgcaat cgttacggcg
     1681 ctaaatttca ttcccgcgct gttcatttat cgcgagccgg aacgcgtcgt tcccgccgag
     1741 ggccaaaagg ggccgctggg cgtatttgtg acctcactgg cgatgctgct gcgcgatgtg
     1801 cgtttcgtgc tgtttctgct gatcgcggcg tgcttctggt tcatgttcat gcagctttgg
     1861 gacctgctgc cgaacttctt gaacgagtgg gtggacacca gcgcggttgg ccgcgtgctg
     1921 aaatcgatct tcggcgaaaa cctgacctgg gtgctgccga atgggcaggt aaaacccgaa
     1981 atgataatca acattgacgc gacctcgatc cttctgttcg tactggtgat ctcctggatg
     2041 atccggcgga tgcacaaaat tgcggcgatg atcctgggca tgctgatctc gctggtcggc
     2101 tttgtcggag cgggggcgac caacgtgggc gcgatctgct gcctgatgat cttcgtgttc
     2161 gcgatcggcg agatgatttg cagcccgaca ttcagcgcgt acatcggcct gatcgcaccg
     2221 ccggctcgca aagcgcttta catgggctat tcgaacatac cgtttgcgat tggctggggc
     2281 atgggcaact tcgtctcggg tcctatgtac gatcagatcg gcagcaagga aatgctggcc
     2341 cgcgggcacc tcgtgcaggt gtatcagttg cccgccgaac tggcgacgaa ggacaataag
     2401 gctttcccga gcgaccaggt tgttcccagc ttggcctacg tgaccaaaac cggcaacgtg
     2461 gagaacctgc gcgagatcat cgcgagcttt gccggccaag aaaagcccga tgccgaagcc
     2521 ctcaagcagg cctacgcgcc gattcgcgaa caggtcagcc cggccgacct ggacgnnnnn
     2581 nnnnnacccg cagcgcgtct ggtattacct gggcgcgttc gggctcgcag gcacggtggg
     2641 tatgatcatc ttttacctgc tggcacgcgc gccgagcggc gaggcggaac cggccgctta
     2701 gtccgccggc tgtacggcgc cgcgacgcgg agcgcgggtg cgctgatggg acccgatgaa
     2761 taatccttcc accaacgccg tccagccttc gtcaatcgag ctgccgcccg aagctgatgc
     2821 ggctgtggcg atcgtgcgca agctggtcga cgccgggcac accgcgctgc tggccggcgg
     2881 gtgcgtgcgc gacttgctgc tggggcatcc accgcaggat tacgacgtcg cgaccagtgc
     2941 ccggcccgag cagatcgtgc agctcttccg gagcacccgg ctggtcggcg cgcaattcgg
     3001 ggtcgtgctg gtgcgcaagc ggcggcgctg ggtggaggtt gcgacgtttc gctgggatgg
     3061 gccgtacctc gacgggcggc ggcccagcaa ggtcgtcttc agcgatgccc gcgaagacgc
     3121 cctgcggcgc gacttcaccg tcaacggcat gttcttcgat ccgctggccg gccaagtgat
     3181 cgaccacgtc ggcgggcagc cggacctgag ggccggcgtt atccgtgcca tcggcgaacc
     3241 ttcggcgcgc ttcgaagaag atcacctgag gctgctgcgg gcggtgcgtt tcgccgctcg
     3301 gctcaatttc ccgatcgagc cgcagacctt cgctgcgctg cgtgaacatg cgcctcagtt
     3361 agccaaggtc gcggttgagc gcgtgcggga tg
//
LOCUS       DAOSYS010000331         3327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_22700_length_3327_cov_5.536369, whole genome shotgun sequence.
ACCESSION   DAOSYS010000331 DAOSYS010000000
VERSION     DAOSYS010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22700_length_3327_cov_5.536369"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLU99_14810"
     rRNA            <1..371
                     /locus_tag="PLU99_14810"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            510..618
                     /gene="rrf"
                     /locus_tag="PLU99_14815"
     rRNA            510..618
                     /gene="rrf"
                     /locus_tag="PLU99_14815"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(850..1062)
                     /locus_tag="PLU99_14820"
     CDS             complement(850..1062)
                     /locus_tag="PLU99_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18364.1"
                     /translation="MSAERISEFLERYPFQPFRIRFANEAPVDVYNPALVVLKQRELF
                     VANWTRDYFHLYWLTDVVEIEALHAA"
     gene            1294..2967
                     /locus_tag="PLU99_14825"
     CDS             1294..2967
                     /locus_tag="PLU99_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOO18365.1"
                     /translation="MSRDVELVKNAAGRLVPAIVNGQPQTPFQGVGKFRPTGRKAAPP
                     VRTSADYPADGNKITAYVKTAATPADLLPARAADCSSAVEAFKVALRRAGARDGMRIS
                     SHHHFRNGDKLAVPLFQAAAELGLKNLIWFPSACFPCHAPLIPLIESGVIDRVEGSMN
                     GPIGDYCSTGKMRGMGVLRSHGGRWQAVQDGEVHIDIAVLAAPTADPFGNSNGVNGPS
                     ACGGLGFGLVDSLYADHVIMVTDNPVPFPCVPWQIQGNNVDQVVVLESVGDPAKIVSG
                     TTELTKSPDRLMIAEMAAQFARDAGLLHDGFSMQAGAGGTALAFAIYLRDYMRAQKIK
                     ARFIRGGSTKYLTEMLEEGLTDYILDGQTFDLFGVRSMRDNPRHVMTSPFTSYNWHGK
                     GHFATFVEIAVLGATEVDLNFNANVVTHSDGRLLHGIGGWQNCLAAKCVILPIPSVRD
                     RIPVIVDNVTTLCGPGELIDVIVTERGIAVNPRREDLLNALQENGSKLPIRPIAEIKK
                     EVDAMCGGPPAPPVFGDKVVAAIKWVDGTVIDCVKAVEVRGPASGGGGE"
BASE COUNT          631 a         1069 c         1023 g          604 t
ORIGIN      
        1 ggtcccaagg gttcggctgt tcgccgatta aagcggtacg cgagctgggt tcagaccggc
       61 gtgagccagg tcggtcccta tctgctgtgg gcgtatgagt cttgaagggc catcactcta
      121 gtacgagagg attgggtggt acgggcctct ggtgtacctg ttgtcgcgct agcggcacgg
      181 cagggtagct aagcccggac tggataaacg ctgaaggcat ctaagcgtga agccgtccct
      241 aagatgagga ctcggtgctc cccttcgggg tgagctgaag acccctggta gaccaccagg
      301 ttgataggcc gggtgtgtac gagctgagaa gctcttagct aaccggtact aacggtcgaa
      361 ggcttgacca cgtttatgaa tcgctgtggt cgtcactgat gcattccgtg ccataacgga
      421 cttgcgcaac cagagacctc gcattcacct tgaacttctc cgcctaaaaa tttcgcttca
      481 ccggcgattc ttggtgaagc gcacgagctg ccggtaacca tgcgtgtggg gaaacacctg
      541 atcccattcc gaactcagta gttaagccca cccggccgat ggtagtcctg atcgggcgag
      601 agtaggtgat tgccggcttt taaggcccgg cgacgttaac cgcgtcgccg ggcctccttt
      661 atttctaagg atgcttggac aaaacctacc cctcgcgtgc tgtgcgcgga gcaccggcca
      721 ctggaacagc gcctcacccc ccttccccct gggttcgtcc ggccaacccc tcgttccagg
      781 tccgttcggc caacatagcg gccgtggcct gagctgcata ggctcccgcg tcagcagccg
      841 gcacacgcat cacgcagcgt gcaacgcctc gatctcgact acgtccgtca gccagtaaag
      901 atggaagtaa tcgcgcgtcc agttcgcgac gaacagctcg cgctgcttta gcaccactaa
      961 cgccgggttg tagacatcaa caggcgcttc gttcgcgaac ctgatgcgga acggctgaaa
     1021 cggatagcgt tccaggaact cgctgatcct ctccgcactc atagcaccgt tttagcatcc
     1081 ggggcgggct cgaaacaatc ctttttttcg cccgataagc gtgttcgtcg gcggcgccag
     1141 tgcctgaagc cgccgcttca cgcagtgccc ttccggccgg gggcggcgct tccgccgagc
     1201 gcggcctgac ctgccggacg ttattgtgct accatgtcgc ttttcatccc ggcactggtg
     1261 cccgggactc gctcgtgcag caaagggaag gctatgtcgc gcgacgtcga actcgtgaag
     1321 aacgccgccg gacgcttggt tccggccatc gttaatggtc agccgcagac cccgttccag
     1381 ggcgtcggca aattcagacc caccgggcgc aaggccgctc cgcccgtacg gaccagcgcc
     1441 gactacccgg cggacgggaa caaaataacc gcctacgtca aaaccgccgc tacccccgct
     1501 gaccttctgc cggcgcgggc cgcggattgc tcctcagcgg tcgaggcctt caaggtcgcg
     1561 ttgcggcgtg ccggggcgcg cgacggaatg cggatttcgt cgcaccacca cttccgcaac
     1621 ggcgacaagc tggcggtccc gctgttccaa gccgcggccg agctgggcct gaaaaatttg
     1681 atctggttcc cgtcggcctg ctttccgtgc cacgctccgc tcatcccgct gattgagtcg
     1741 ggcgtcattg atcgcgtcga aggcagcatg aacgggccga tcggcgatta ctgctcaacc
     1801 ggcaagatgc gcggcatggg cgtcctgcgc tcgcacggcg ggcgctggca ggcggtccag
     1861 gacggtgagg ttcacatcga tatcgcggtg ctcgcggcgc ccaccgctga tccgttcggc
     1921 aactccaacg gcgtaaacgg cccgtcggcc tgcggcggcc tgggcttcgg cctggttgat
     1981 tcgctctacg ccgaccacgt gatcatggtg acggacaacc cggtaccgtt cccgtgcgtg
     2041 ccctggcaaa tccagggtaa caacgtcgac caggtcgtcg tgctcgaatc ggtcggcgac
     2101 cccgccaaga tcgtcagcgg cacgaccgag ctgaccaaaa gccccgaccg gctgatgatt
     2161 gcggaaatgg ccgcccagtt cgcgcgcgac gccggcctgc tgcacgacgg cttctctatg
     2221 caagccggcg ccggcggcac cgccctggcg ttcgccattt atctgcgcga ctacatgcgg
     2281 gcccagaaga tcaaggcccg ctttatccgt ggcggcagca cgaagtacct gaccgagatg
     2341 ctcgaggagg gcctgacgga ctacatcctc gacggccaaa cgttcgacct gttcggcgtg
     2401 cgcagcatgc gcgacaaccc gcggcacgtc atgaccagcc cgttcaccag ctacaactgg
     2461 cacggcaaag gccatttcgc gacgttcgtg gagattgcgg tcctaggcgc gaccgaagtc
     2521 gacctgaact tcaacgccaa cgtggttacg cactccgacg gccggctgct gcacggcatc
     2581 ggcggctggc aaaactgcct ggcggccaag tgcgtcattc tgccgatccc gtcggtccgc
     2641 gatcgcatcc cggtgatcgt cgacaacgta accacgctct gcggcccggg tgagctgatc
     2701 gacgtgatcg tgaccgaacg cggcattgcg gtgaacccgc gccgtgagga tcttctgaat
     2761 gcattgcagg aaaacggctc gaaactcccc atccgcccga tcgccgaaat caaaaaggaa
     2821 gtagacgcca tgtgcggcgg cccccctgcc ccgccagtct tcggcgacaa ggtagtcgcg
     2881 gccatcaaat gggtcgacgg cacggtaatc gactgcgtaa aggccgtaga agtccgcggc
     2941 ccggccagcg gcggcggcgg agagtgaccg cctgaccgcg caggcggtat aatactgcct
     3001 gtgcggaggc ccacgcagtg gacattgagc ggcaagtaga gtactggcgt tccggcagcg
     3061 cagaggattt cgctgccgcg gtttcattgg ccgacgccta tccttgacac gcgctgttct
     3121 tcgcagactt ggcgctggaa aaaatgctta aggcaaatga acgggcggtt gcctttaacc
     3181 tccgccccac agacgcggca gcgagagaat ggcgacgccg gcgatggcca caatggcgcc
     3241 ggccacggcg cggaagccta tgtgctcctt gtgaacccag cgcgaaaacg gaaggatgaa
     3301 caccggcggt aacgacagca gcgtctg
//
LOCUS       DAOSYS010000332         3304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_22876_length_3304_cov_4.257618, whole genome shotgun sequence.
ACCESSION   DAOSYS010000332 DAOSYS010000000
VERSION     DAOSYS010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_3304_cov_4.257618"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PLU99_14830"
     CDS             <1..1484
                     /locus_tag="PLU99_14830"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOO18366.1"
                     /translation="VLAKEFPGGVLVMTGANSAVGLRSMAARYLFLDEIDGYPGDVDD
                     EGDPIQLVAARARTFARRKIYLVSTPKITGLSRIEAAFEESDQRRYWVPCPVCGEYQT
                     LKFAQLTWPKGKPKQAVYVCEHCSARLENHQKHTMLARGEWRPSAVGDGRTAGFWLSS
                     LYSPVGWFSWADAAEMFEKARKNPTLLQVFVNTVLGETWAEAGDAPDWQRLYDRRENY
                     RLGTVPAGGLFLVAGCDVQRDRLEIALVAWGRNRENWLTDYVVLDGDTSRPEVWDRLT
                     DLLNTTYPHACGARLGIVRMAVDSGFATQQVYAWAREQGPGRVLVTKGYETGSAPIGQ
                     PSAVEVTLDGRKIKRGVKVWPVATGMLKSELYGWLKLERPTAESGKPCPPGYCHFPQL
                     PEEFFKQLTAEQLVPKVVKGYRKLEWVKTRERNEALDTYVLCRAAATQFGMDRFGERH
                     WKALEEQIGSASREPVEQSEPPALPKPRALPQRRIIRSRFLDR"
     gene            1487..1690
                     /locus_tag="PLU99_14835"
     CDS             1487..1690
                     /locus_tag="PLU99_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18367.1"
                     /translation="MYTEQQLQALRDALANGVRRVRFGDREIEYRTIDELKAAIAAAE
                     ADVSKSSGVPVTRHIRVSTEKGF"
     assembly_gap    1871..1970
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1971..3005
                     /locus_tag="PLU99_14840"
     CDS             <1971..3005
                     /locus_tag="PLU99_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOO18368.1"
                     /translation="PRLPKDGLSVPLQLQLLEAEHLPTNETRKLENGNYIRAGIEFNG
                     IGKRVAYHLYREHPGDALNPMASTELVRVPAESVLHLFRPIRPGQLRGQPWLTQVLIK
                     LYELDQYDDAELVRKKTAAMFAGFVTKNAPEDVLVGEGAADANAVALTGLEPGTMQVL
                     LPGEDVKFSNPADVGASYETFMRVQLRSIAAGMGITYEQLTGDLTGVNYSSIRAGLLE
                     FRRRCEQFQHQVIVFQMCRPIWRAWIDAAVLAGALPAGDGNYDVKWIPPGFAWVDPLK
                     DIKAQIMAVRAGFKSRAEVVSEQGYDAEEIDREIAADNTRADQLGLEYDSDPRKETND
                     ASAASGGADL"
     gene            2968..>3304
                     /locus_tag="PLU99_14845"
     CDS             2968..>3304
                     /locus_tag="PLU99_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOO18369.1"
                     /translation="MTPLPHLAARIFDTPLLIAPQKLEVILAVLAPRLGLDVPAPAVA
                     ATSERRVRKPYEVTPDGIAVIPIEGTLVHKAYGLDALSGLRSYVDIQEEVEDAATDPA
                     IKGILLDIDS"
BASE COUNT          581 a         1008 c         1088 g          527 t
ORIGIN      
        1 cggtcctggc gaaagaattc cccggcggcg tgctggtgat gaccggcgcc aacagcgctg
       61 tcggcctgcg ctcgatggcg gcgcgttacc tgttcctgga cgaaatcgac ggctacccgg
      121 gcgacgtcga cgacgaaggc gatccgatcc agcttgtggc ggcccgtgcg cggaccttcg
      181 cgcggcggaa gatttatctc gtatcgacgc ccaagattac gggattgagc cggatcgagg
      241 ccgccttcga ggaaagcgat cagcggcggt actgggtgcc gtgcccggtt tgcggcgaat
      301 atcagacgct gaagttcgcg cagctcacct ggcccaaggg caagccgaag caggcggtat
      361 atgtctgcga acactgctcg gcgcgcctcg agaatcatca gaagcacacg atgctcgcgc
      421 gcggcgagtg gcggcccagc gcggtgggcg atgggcgcac ggcgggattc tggctgtcca
      481 gcctctacag tcccgtgggt tggttcagtt gggccgatgc cgcggagatg tttgagaagg
      541 cgcgcaagaa tccaacgctg cttcaggtct tcgtcaacac cgtgctcggg gagacgtggg
      601 cggaggcggg cgatgcgccc gactggcagc ggctctatga ccggcgtgag aattaccggc
      661 tgggcacggt gccggctggc ggattattcc tggtcgccgg ctgcgacgtg cagcgggatc
      721 gcctcgaaat cgcgctggtc gcctggggcc gcaatcgcga aaactggctc accgattatg
      781 tcgtcctgga tggcgacacc tcccggcccg aggtttggga ccggcttacc gatctgctga
      841 atacgaccta cccgcatgcg tgcggggcgc gactgggcat cgtacggatg gcggtcgact
      901 ccggcttcgc tacgcagcag gtctatgcat gggcccgcga gcaggggccg gggcgcgtgt
      961 tggtgacgaa aggctacgag accggctcgg cgcccatcgg ccagcccagc gcggtcgaag
     1021 tgactctcga tggccggaag atcaagcgcg gggtaaaagt atggccggtc gcgaccggaa
     1081 tgctcaagag cgaactgtac ggctggttga agctggagcg gcccactgcg gaaagcggca
     1141 aaccatgccc gcctggttac tgccacttcc cgcagttgcc cgaggagttt ttcaaacagc
     1201 tcacggccga gcagctggtg ccgaaggtgg tgaaaggcta ccggaagctc gagtgggtga
     1261 agacgcggga gcgaaatgag gcgctcgaca cgtacgtttt gtgccgcgcg gccgccacgc
     1321 agttcggcat ggatcgcttc ggcgagcgtc attggaaggc gctcgaagag cagatcggca
     1381 gcgcttcgag ggaaccggtg gagcagagcg agccgcctgc gcttcccaag ccacgagcgc
     1441 tcccccagcg gcgtatcatt cgctcccgtt ttctggaccg ctgaccatgt acaccgagca
     1501 gcaattgcaa gccttgcgcg acgctctcgc gaacggcgtg cgccgcgtcc gcttcggcga
     1561 ccgcgagatc gagtaccgca ctatcgacga attgaaggcg gcaatcgcag cggccgaggc
     1621 cgatgtcagc aagagcagcg gagtgcccgt gactcgacac atccgtgtct cgacggagaa
     1681 gggcttctga catgggcctc tggagccgaa tgaaaacctg gctgccgggc atgcgagccg
     1741 ctgccgatta cgaagcggct gctatgacgc ggcgcacaac cggctggtcg ccggtcgcga
     1801 gcgacgtcaa cacgctcgtc ttccgaaacg cggacacgct gcggtcgcgc tcgcgcgaca
     1861 tggtgcggcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccgcggctgc
     1981 cgaaggacgg cctttcggtt cccttgcagc ttcagttgct cgaggccgag catctgccga
     2041 ccaacgaaac gcggaagctc gaaaacggaa actacatccg cgccggcatc gagttcaacg
     2101 ggatcgggaa gcgcgttgcc tatcatcttt atcgagaaca tcccggcgac gctctcaacc
     2161 cgatggcctc gacggaactg gtgcgagtgc ctgccgaatc cgtcctgcat ctgttccgcc
     2221 cgatccgccc gggccagttg cgcggccagc cctggctgac gcaggttctg atcaagctgt
     2281 acgaactcga ccagtacgac gacgcggagc tggtgcggaa gaagaccgcc gcgatgttcg
     2341 ccggcttcgt cacgaagaac gcgccggagg acgtgctggt cggcgagggc gcagcggatg
     2401 cgaacgcggt cgcgctcacc ggtttggagc ccggaacgat gcaagttctg ctgccgggcg
     2461 aggacgtgaa gttctcgaat cccgccgacg tcggcgcgag ctacgagacg ttcatgcgcg
     2521 tccagttgcg gtcgatcgcc gccggcatgg gcatcaccta cgagcagttg accggcgacc
     2581 tcaccggcgt caactactcg tcgatccgcg ccggcctgct ggagttccgc cgccgctgcg
     2641 agcagtttca acatcaggtc atcgtcttcc agatgtgccg gccgatctgg cgggcctgga
     2701 tcgacgccgc cgtgcttgcg ggcgcgctgc ccgccggcga cggcaactac gacgtgaagt
     2761 ggattccgcc gggcttcgcc tgggtcgatc cgctcaagga catcaaggcc caaatcatgg
     2821 cggtgcgtgc gggctttaag agccgcgccg aggtggtctc cgaacaaggc tatgacgccg
     2881 aggagatcga ccgcgagatc gccgccgaca acacgcgggc cgaccaactc ggcctggaat
     2941 acgactccga cccgcgaaag gaaaccaatg acgcctctgc cgcatctggc ggcgcggatc
     3001 tttgataccc cgctgctgat cgcaccgcaa aagctcgagg taatcctggc ggtgctcgcg
     3061 ccgcgcctcg ggctggacgt ccccgcgccc gccgtggcgg cgacctcgga acgccgcgtc
     3121 cggaagccgt atgaagtcac tccggacggg atcgccgtga tcccgatcga gggcacgctg
     3181 gttcacaagg cgtatgggct cgatgcgctc tccggcctgc ggtcctacgt ggacatccag
     3241 gaggaggtcg aagacgcggc gaccgatcca gccatcaagg ggatcctgct cgacatcgat
     3301 tcgc
//
LOCUS       DAOSYS010000333         3281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23053_length_3281_cov_5.042157, whole genome shotgun sequence.
ACCESSION   DAOSYS010000333 DAOSYS010000000
VERSION     DAOSYS010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23053_length_3281_cov_5.042157"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..993
                     /locus_tag="PLU99_14850"
     CDS             40..993
                     /locus_tag="PLU99_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOO18370.1"
                     /translation="MDGWIILHGGALGDLALTLQLALRLPRVGQDGRVHVVSRTDFGE
                     LSACCPSITRQCADVLGLHWLYSENGAPRPAGLDELIRGAHVLSALGDARGRVHQRLL
                     ALEPARLYSFDPRPRPNLNRHITQQWATDLEAQGLLIPKCIHQHPGRRGLGVPNELRQ
                     RGAALLTAAGAAGRCVLIHPGSGGREKCWPWENFLAVGRALQRAGSEVCFVLGPVELE
                     RQEAANLRAVCDNFPVLHCPPPGDLVAALTAARALIGNDSGPAHLAALLGSPTITIFG
                     PSSPVLWHPLGPEAVTITGDPQHHPADWNIDPIRVAAVCEA"
     gene            complement(1165..3117)
                     /gene="recQ"
                     /locus_tag="PLU99_14855"
     CDS             complement(1165..3117)
                     /gene="recQ"
                     /locus_tag="PLU99_14855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOO18371.1"
                     /translation="MGKAGTLAIPTADILEAVQRCWGFNSLRPLQAQAIQAELEHRDS
                     LVVLPTGGGKSLCYQAPPLVAGRTDVVVSPLISLMKDQVDALRTCGYPAAAIYGKLPL
                     PARLEIEREVMAGLYRLILLAPERLLAPDFLDLLDRVSVRAFAIDEAHCISHWGHDFR
                     KEYRQLAVLRKRFPNASLHAYTATATQRVRDDIAAQLGLRDPLVLVGDFDRANLVYRV
                     IPRMDLYAQVLEVLERHRGEAVIVYCISRKDTETLATRLVTAGVRAAYYHAGMDAEHR
                     RRTQDAFSHEKLDVIVATVAFGMGIDRSDVRCVIHAAMPKSVEHYQQETGRAGRDGLP
                     AECILFYSPADAFKWESLIRKSAEEAGAGPQVVAAALDLLAHMRGYCAAPICRHRFLV
                     EYFGQQYTKDNCGACERCLDEVEADDEANVLAQKILSCVARVEQRFGVGHVVKVLQGA
                     NDRQVRLFGHDRLSTYGLLKEYDAKIITNFTYQLIEQGVLDRTPGDRPILKLNAASWR
                     VLRGEQNVRLVRPKARPLAPTQVEIESWEGVDRQLYESLRGLRNQIAAERGVPAFIIL
                     GNATLRDMARRRPTTPAEFLQVHGIGEQRLREFGARFLEHIRATVGARGTVEPERVAG
                     EGSRDLGIKGSRGAVSPQRPASRPKM"
BASE COUNT          526 a         1137 c         1045 g          573 t
ORIGIN      
        1 tctgctcgcc gtcccttgat ccgttaaaat cgctgcgtga tggacggctg gataatcctg
       61 catggcggcg cgctcggcga tctggcgctg acgctacagc tcgccctgcg cctgccgcgc
      121 gtcggccagg acgggcgggt gcacgttgtc tcccgcactg atttcggcga actgtccgcg
      181 tgctgcccga gcatcacgcg ccagtgcgcc gacgtcctgg ggctgcattg gctgtactcc
      241 gaaaacggcg cccctcggcc cgccggactg gatgaactca tccgcggcgc gcacgtgctc
      301 agcgctttgg gtgatgctcg tgggcgcgtg catcaacgac tgctcgcgct ggagcccgcc
      361 cgcctgtaca gcttcgatcc gcgcccccgc ccgaatctaa accggcacat cacgcagcag
      421 tgggcgaccg atctcgaagc ccagggcctg ctgatcccta agtgcatcca ccaacacccc
      481 ggccggcgcg gcttgggcgt accgaatgag ttgcgccagc gcggcgcggc gctgctaacc
      541 gccgccggcg ctgccggccg ttgcgtcctg atccacccag gcagcggcgg tcgcgaaaaa
      601 tgctggccgt gggagaattt cctggcggtc gggcgcgcct tgcagcgcgc cggctcagaa
      661 gtgtgcttcg tactgggccc ggtggagttg gagcgacaag aggccgccaa cctgcgcgcg
      721 gtttgcgata acttccccgt gctgcactgc ccgcccccag gcgacctggt tgccgctctg
      781 acggccgcac gggccctgat cggcaacgac tccggcccgg ctcacctggc ggcgctgctc
      841 ggcagcccga cgatcaccat cttcggcccc tcctcgccgg tcctatggca tccgctcggc
      901 cccgaagccg tcaccatcac cggcgacccg caacatcatc cagccgactg gaacatagat
      961 cccattcgtg tcgcggccgt gtgcgaagcg taggcggcgg ccccacgccc cgagcctgcg
     1021 ggacctgatc cgagcccgag gcgccagcga gggcaaccga cccgcgcccg acacgcaagc
     1081 gagcgcgctc tgagggtgtg gggagcgggt actgcgctgg cgggaacttt tggcggcatt
     1141 ccggggcgac acgccccggc tctgctacat tttggggcgg gacgccggtc tctgcggcga
     1201 cacggcgcct cttgatccct tgatccctag atccctcgat ccctctccgg cgacacgttc
     1261 cggctcgact gtgccgcgcg ctccgactgt cgccctgatg tgctccagaa accgggcccc
     1321 aaattcccgc agccgctgct cgccgatgcc gtgtacctgc aggaactcgg ccggcgtggt
     1381 tgggcggcgg cgggccatgt cccgcagtgt ggcattcccg agaatgatga acgccggcac
     1441 gccgcgttcg gccgcgatct gatttcgcag cccgcgcagg ctctcgtaca gttggcgatc
     1501 cacgccttcc caggattcga tctccacctg cgtcggcgcc agcgggcggg ccttaggccg
     1561 gacgagcctc acattctgtt cgccgcgcag cacgcgccat gaggccgcat tcagcttgag
     1621 gatcgggcgg tcgccgggcg tgcggtccag gacgccctgt tcaatgagtt gatacgtgaa
     1681 gttggtaatt atcttggcgt catattcctt cagaagtcca taggtgctga gccgatcatg
     1741 cccaaacaag cgcacctgtc tgtcattggc accctgcagc actttgacga catgccccac
     1801 cccgaagcgc tgttcgacgc gcgccacgca ggaaagaatt ttctgcgcca atacattggc
     1861 ctcgtcgtcc gcctcaactt catccagaca gcgctcgcaa gccccacagt tgtctttcgt
     1921 gtattgttgt ccgaagtatt caaccagaaa acgatggcgg cagatcgggg cggcacagta
     1981 gccgcgcatg tgcgccagca agtccagcgc ggcggcaacc acctgtggcc ccgccccggc
     2041 ctcttccgca ctcttgcgga tcaggctttc ccatttgaac gcgtccgcgg gcgaataaaa
     2101 cagtatgcac tcggccggca gaccgtcgcg cccggcgcgg ccggtctctt gttgatagtg
     2161 ttccaccgat ttgggcattg cggcgtgaat cacgcagcgc acgtcgctgc ggtcgatgcc
     2221 catgccgaag gcgactgtgg ccacgattac atccagcttt tcatgcgaga acgcgtcctg
     2281 cgttcggcgg cggtgctcgg cgtccattcc ggcgtggtaa tacgccgccc gcacgccggc
     2341 ggtgaccaac cgcgtcgcca gggtttcggt gtctttgcgg ctgatgcagt aaacgatgac
     2401 ggcttcgccg cgatggcgct cgagcacctc gagcacttgc gcgtacaggt ccatgcgcgg
     2461 gatcacgcga tacaccaggt tggcgcggtc gaagtcgccc accagcacca gcggatcgcg
     2521 cagaccgagt tgggcggcga tgtcgtcgcg gacgcgttgc gtcgcggtag cggtgtaagc
     2581 gtgcaggctg gcgttgggga aacgcttccg cagcacggcg agctgccggt actccttacg
     2641 gaaatcgtgc ccccagtggc tgatgcagtg cgcctcgtca atcgcgaacg cccgcactga
     2701 gacgcggtcg agcaggtcga ggaagtcggg cgccaaaagc cgttccggcg cgagcaggat
     2761 tagccggtac aggccggcca tgacctcccg ctcaatctca agccgcgcgg gcagcggcaa
     2821 tttcccgtaa atcgcggcgg ccggatagcc gcaggtgcgc agggcgtcca cctggtcctt
     2881 catcagcgag atcaggggcg agacaacgac gtcggtccgc ccggcgacca ggggcggcgc
     2941 ctggtagcag agtgactttc cgccgcccgt aggcaggacg accagcgaat cgcgatgctc
     3001 cagctcggct tggatggctt gagcttgcag gggacgcagg ctgttgaacc cccagcagcg
     3061 ttgcacggcc tccagaatgt cggccgtcgg aatcgccagt gtgccagcct tccccatgcc
     3121 cgcgtagatt acagcaggac gcgggcccgg tcacgccagg ccgccccccg ccggcacccg
     3181 ccgatacgcc tccaactcct gcgttcgccc gttgactagg gcaaccggaa cgactacatt
     3241 gccgtctctg ggcttcgtcc cggagtttac gcccgtgagc g
//
LOCUS       DAOSYS010000334         3231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23552_length_3231_cov_4.729534, whole genome shotgun sequence.
ACCESSION   DAOSYS010000334 DAOSYS010000000
VERSION     DAOSYS010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23552_length_3231_cov_4.729534"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLU99_14860"
     CDS             complement(<1..527)
                     /locus_tag="PLU99_14860"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOO18372.1"
                     /translation="MNFRTNVKTVAVIGTGTMGCGIAQVFAQAGRKVLMYDAVPGAVE
                     RALKTIDKSLSKFVEKGKLTADAAGAAKANLKPAGLSDMKDADLIVEAIFEDVPTKRA
                     LYAELAKVVPPDVVFASNTSSISISELAAASGRPEMFIGMHFFNPVPMMQLVEVVVGL
                     KTAEPVVEFTMNLAK"
     gene            complement(847..2025)
                     /locus_tag="PLU99_14865"
     CDS             complement(847..2025)
                     /locus_tag="PLU99_14865"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOO18373.1"
                     /translation="MAKEYAYIVAGKRSPIGKFLGSLSPLSAAQIGSQVAKALLDYAK
                     ADRSAFDEVYIGQVLQAGCGQIPSRQVALGAGIHDKISTCTLNKMCGSGLQSVMFADM
                     AIRAGDADLVLAGGIECMSQAPYVIRSMRAGSKFGHVEAIDSMLHDGLTDAYTNEAMG
                     ELADYTAEKAGITRREQDEWAVRSQQRAAAASQAGKFKEEIVPIELPRNKGFLSVDET
                     FRADTTVEALSALKPAFKKDGTVTAGNSSQLSDGAGMVVVASERGLKKCGMGPMARIV
                     GIATAGGPPRDLFFTPPVAVRMACQKAGWSLNQVDFFELNEAFASQTIAGVRGLELSG
                     ENVNVNGGAIALGHPIGASGARVLVTLLHILKAQKAKRGVAALCLGGGNAVAMAVEMV
                     "
     gene            complement(2030..3217)
                     /locus_tag="PLU99_14870"
     CDS             complement(2030..3217)
                     /locus_tag="PLU99_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOO18374.1"
                     /translation="MDFRFDDDQRQMRDMLRSFCNERIAPKAEQRDHEGRFEDGLIEM
                     LGEMGLFGTYVPEQYGGTGQDIPTYLMTVEELSRACGATGILVSAHHSLCVDPILNFG
                     NEDQKRKFLPKLARGEWIGCFSLTEPGSGSDAGAARCMAIETDRGWLINGTKSFVTNG
                     VQAHVIVLFAVTDPDNPKRRMSAFIVEKNTPGCSIGKVEKKMGIRASSTTELVFEECL
                     IPRENLLGERGRGLPIALATLDGGRLGVAAQAVGISQAALEAATAYSKERVQFGAPIC
                     NLQAIQWKLADSAVWIAAARALMYRGGWMKQRYMDEKNPSLRISYSAEAAMAKLYASE
                     ASSRVTNAALQVYGGYGYCQDYPAERLLRDAKITELYEGTSEIQRLVIARQILNDPQW
                     VTR"
BASE COUNT          564 a         1114 c          929 g          624 t
ORIGIN      
        1 tctttggcga gattcatcgt gaactcgacc accggctcgg ccgtcttcag accgacgacg
       61 acctcgacga gctgcatcat cggcaccggg ttgaagaagt gcatgccgat gaacatctcc
      121 gggcggccgc tggcggcggc gagttcgctg atgctaatac tgctggtgtt gctggcgaag
      181 acgacgtccg gcgggacgac cttggccagt tcggcgtaca gggcgcgctt agtcggcacg
      241 tcctcgaaga tggcctcgac gatcaggtcg gcgtccttca tatctgacaa gcccgccggc
      301 ttgaggttgg ccttggcagc cccggccgcg tcggcggtca gcttgccttt ctcgacgaac
      361 ttcgaaagcg atttgtctat ggtcttgagc gcccgctcga ctgcgccggg cacggcatcg
      421 tacatgagca ccttccgccc cgcttgggcg aagacctgag cgataccgca gcccattgtt
      481 ccagtgccga tcacggcgac ggtcttgaca tttgtgcgaa aattcacgag cagtctcctc
      541 caacgcgttt ggggttcaat ccgccggggc gcgtgtttgc tccgcacagc gcccttcgag
      601 cattcaggag cggcctatcc gtggacacgc cgcgaagacc agacgccggc cgcgatggtc
      661 agtatgaccc agccgcgaac ggaactggcg tgagcacaat actcagcaac cccagactga
      721 ccagcagcgc ggcagcggca gaagcgccca gccgactgta cagcgcctcc ccggccagcc
      781 ccgaggcacg ttcggcccgt gcggcgctca cgtatcccag ccacgcggcc cgtaagtcgc
      841 cggccgctac accatttcca cggccatggc caccgcattc ccgccgccca agcacaatgc
      901 cgcgacgccg cgcttggcct tctgcgcctt caatatgtgc agcagcgtga ccagcacgcg
      961 ggcgccgctg gcgccgatcg ggtgacccag cgcgatcgcg ccgccgttga cgttcacatt
     1021 ctcgccactg agttcgaggc cccgcacgcc cgcgatcgtc tgtgacgcaa acgcctcgtt
     1081 cagctcgaag aaatccacct ggttcagcga ccaaccggct ttctggcagg ccatgcgcac
     1141 cgcgaccggc ggcgtgaaga acaggtcgcg cggcggacca ccggccgtgg ctatgccgac
     1201 gatccgcgcc atcggcccca tgccgcactt cttgaggccc ctttcactgg ccaccactac
     1261 catcccggcc ccgtcggaaa gctgcgacga gttgccggcc gttacagtgc cgtctttttt
     1321 gaatgccggc ttgagcgccg agagggcctc caccgtcgta tccgcccgga acgtctcatc
     1381 aaccgaaaga aagcccttat tccgcggcag ctcgatcggg acgatctcct ccttgaactt
     1441 gccggcctgc gacgccgccg ccgcccgctg ctggctgcgc accgcccact cgtcctgctc
     1501 gcgccgcgtg atgcccgctt tctcggctgt gtagtcagcc agttcgccca tggcctcatt
     1561 cgtgtaggcg tcggtcagcc catcgtgcag catcgaatcg atggcttcga catgaccgaa
     1621 cttactgccg gcccgcatgg aacggattac gtacggcgcc tggctcatgc actcgatgcc
     1681 gccggccagt accaaatcgg catcgccggc gcggatcgcc atatcggcga acatcacgga
     1741 ttgcagcccg ctgccgcaca tcttgttgag ggtacaagtc gaaattttgt cgtgaatgcc
     1801 ggcccccagg gccacctgcc gggaaggtat ctgcccgcag ccggcctgca acacctgccc
     1861 gatgtagact tcatcaaagg cgctgcggtc ggccttggca tagtccagca gtgctttggc
     1921 gacctggctc ccaatctggg ccgcgctcag cggcgaaaga ctgcccagga acttgcctat
     1981 ggggctgcgc ttgccggcga cgatgtacgc gtactctttg gccatgagtt tacctcgtta
     2041 cccattgggg gtcgttcagg atctgtcgtg cgatcacgag cctctgaatt tcactggtac
     2101 cctcgtacag ctctgttatt ttagcatccc gcagcaggcg ttccgcggga taatcctggc
     2161 aatagccata gccgccatag acctggagcg cggcattcgt gacccggctc gaggcttcgc
     2221 tcgcgtacag tttcgccatg gccgcttcgg cactgtatga aatccgcaag ctcggattct
     2281 tctcgtccat gtagcgctgc ttcatccagc cgccgcgata catcagcgct cgcgccgcgg
     2341 cgatccagac cgcgctgtct gccagcttcc actgaatggc ctggagattg cagatcgggg
     2401 cgccgaactg cacgcgctct ttactatagg cagtcgccgc ctccagcgct gcctgcgaaa
     2461 tgcccaccgc ctgggccgcc acgcccagcc gcccgccatc cagcgtcgcc agtgctatcg
     2521 gcagaccgcg gccgcgctcc cccagcaggt tttcgcgtgg aatcaggcat tcctcgaaca
     2581 ccagttcggt agtgctgctg gcgcgaatgc ccattttctt ttcgactttg ccgatgctgc
     2641 aacccggcgt gtttttctcc acgatgaatg cggacatacg ccgtttggga ttgtcggggt
     2701 cggtcactgc gaagagaacg atcacatgcg cctgaacgcc attggtgacg aaactcttcg
     2761 ttccgttgat cagccagccc cggtccgttt caatcgccat gcagcgggcc gctcccgcgt
     2821 cgctgccgct gcccggctcc gtcaaggaga agcagccgat ccactcgccc cgcgccagct
     2881 tgggcagaaa cttgcgcttc tggtcctcat tgccaaaatt gaggatcggg tcgacgcaca
     2941 gcgagtgatg ggccgagacc agaatgcccg tggcaccgca ggcgcgcgac agctcctcca
     3001 cggtcatcaa atacgtcgga atgtcctgcc cggtcccgcc atactgttcg ggaacatagg
     3061 tcccaaacaa ccccatttcc ccaagcatct cgatcaagcc gtcttcgaac cggccctcgt
     3121 gatcgcgctg ctcggccttg ggggcgattc gctcgttgca gaaactgcgc agcatgtcgc
     3181 gcatctgccg ctggtcatca tcgaagcgaa aatccacgtt aggacctcgt t
//
LOCUS       DAOSYS010000335         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23601_length_3226_cov_5.786818, whole genome shotgun sequence.
ACCESSION   DAOSYS010000335 DAOSYS010000000
VERSION     DAOSYS010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23601_length_3226_cov_5.786818"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..1760
                     /locus_tag="PLU99_14875"
     CDS             408..1760
                     /locus_tag="PLU99_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOO18375.1"
                     /translation="MPPTAFILILTADEEKGRRLRDQLREHHNHPSVVVDSLERALDS
                     IRQRTPDVVVAEARINGKETAKPLADLLDRLARDATLLVLGEPAVRPTPQRIDLEVLR
                     PDSSDESLVHKISSVAGSAAARRDDRLLQQSLAEVPAFEGIVGHTPQIRRIIERIKKA
                     ARNKLTVLILGETGTGKDLIAEAIHKQSDRSRRALKSVNCAGLNENLLESELFGHVKG
                     AFTGAVSDRKGYFVAADGGTLFLDEVGDMPMTMQAKLLRVLERREITPVGSTEVQRVD
                     VRVIAATNTDLRQAVEEKRFREDLYYRLKQWEINVPPLRERQQDIPLLAMHFLKRANE
                     THGVNVPGFSADAMAHLVHHYWPGNVRELANLIEAVAVEVENRRIETDDLPESIRGRL
                     DIVPVSAGSMVGLPLAQIERIMIERTLETTGGNREAAAKMLGIGTRTLYRKIKEYDLP
                     "
     gene            complement(1876..2998)
                     /gene="prfB"
                     /locus_tag="PLU99_14880"
     CDS             complement(join(1876..2916,2918..2998))
                     /gene="prfB"
                     /locus_tag="PLU99_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146683120.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOO18376.1"
                     /translation="MATVQFEDPAAVLHDLTTRIHAIRDSLDLPAKSARIDELTKRMA
                     QPGFWDIQENAQKVVQELKGLRAVVDPVRELLAKSEDAGVLLELAREQSDADAEAEVQ
                     RELEQLAAKTDQVELLTLLSGPNDARPCFLSIQAGAGGVDSCDFAEMLLRMYVMYFEK
                     NGYDVAEVDRRDGEEAGIQSVTLRVEGPYAYGYLSCEAGVHRLVRLSPFNAQGKRQTS
                     FVGVDVLPEIEETKIELPEGDLDIEYFRRASGAGGQNVNKVATAVRIRHKPTGIMVEC
                     VNERSQAQNKRMALSILKSKLEQIEQAKRDAELAKMYSEKGDIAWGNQIRSYVLHGST
                     IHVKDHRTNLAIGDTQRVLDGDIQPFIDARLRQKLKAGA"
BASE COUNT          582 a         1088 c         1004 g          552 t
ORIGIN      
        1 ctgtacatct cgaccagccg ctccgcacac cacgccagga tgccgaggcc ccccaacaaa
       61 gcccccacct tcagcaccac cgtcagtagc ccggcgcgct ttgcctcggc cggctcgctc
      121 ctctcatccg cttcgtccat gccgaactcc atatccgccg aattatttgc ggcgaccggg
      181 agacgggcaa ggagccgagc ggtggcaggg ccgcggcgtg acgtcccgga ataccgctaa
      241 gaaacatccc gcacgcccca gtcccgcgtc gtccggagta ccgcgccccc cctaccgcat
      301 gggccgtacc gcataaataa cctgacggca gcgctgggcc aggccgccca aaccggcgac
      361 catccggccc tccgcggccg gctcgtgctg cgttacaatc ctgaaccatg ccgccaacgg
      421 cgttcatcct gattcttact gcggacgaag agaagggccg ccgcctgcgc gaccaactgc
      481 gcgaacacca caatcaccct tcggtcgtgg tggacagcct ggagcgtgcc ctggattcaa
      541 tccgccagcg cactccggac gtggtcgtcg ccgaagcgcg catcaatggc aaagaaaccg
      601 ccaagcccct ggccgatctg ctggacagac tggcccgcga cgccacgctg ctcgtgctgg
      661 gcgagccggc cgtacgcccc acgccgcaac gcatcgacct agaagtcctc cggcccgata
      721 gctccgacga gagcctggtg cacaaaatct cgtccgtcgc cggttcggcc gccgcccgcc
      781 gcgacgatcg cctgcttcag cagtcgctcg ctgaagtgcc cgcctttgag ggcatcgtcg
      841 gccacacgcc gcagatccgg cgcatcatcg aacgcatcaa aaaggcggcc cgcaacaagc
      901 tgactgtact catcctgggc gagaccggca ccggcaagga cctgatcgcc gaggctattc
      961 ataagcagtc ggaccgctcg cggcgggcct tgaaatccgt caattgcgcg ggcctgaacg
     1021 agaacttgct cgaaagcgag ctgttcgggc acgtcaaagg cgccttcacc ggggccgtca
     1081 gcgatcgcaa gggctatttc gtcgcggccg acggcggcac gctcttcctc gatgaagtcg
     1141 gcgacatgcc catgacgatg caggccaagc tgctgcgcgt cctggagcgg cgcgagatca
     1201 cgccggtcgg ctcgaccgaa gtgcaacgcg tcgacgtgcg cgtcatcgcc gccaccaaca
     1261 ctgacctgcg ccaggccgtc gaggaaaagc gctttcgcga ggacctgtac taccggctca
     1321 agcagtggga aatcaacgtt ccgccgctgc gcgagcggca acaagacatc ccgctgctgg
     1381 ccatgcactt cctcaagcgg gccaacgaaa cgcacggcgt gaatgtgccc ggcttctccg
     1441 ccgacgccat ggcccacctc gtgcaccact actggcccgg aaacgtgcgt gagctggcca
     1501 acctgatcga ggccgtggcg gtcgaggtcg agaaccggcg aatcgagacg gacgacctgc
     1561 ccgaatcaat ccgtgggcgc ctggacatcg tccccgtctc ggccggcagc atggtcggtc
     1621 tgccgctggc gcagatcgag cggatcatga tcgagcgcac gctggaaacc accggcggca
     1681 atcgcgaagc ggccgcgaaa atgctgggga tcggcacccg gacgctgtat cgcaagatca
     1741 aagagtacga tctgccatag gccgccgcgg accagggatc aagggatcga gggatcaaga
     1801 ggtgtgaaga gcatacctct tggtgcgctc gccttgatgc gctcgccgag ccggcgggat
     1861 ccgctcgttc cccttttatg cgccggcctt aagtttctgc cgcaggcgcg cgtcgataaa
     1921 cggctggatg tcgccgtcga gtacgcgctg tgtatcgccg atcgccaggt tggtgcggtg
     1981 atccttcacg tggatggtcg agccgtgcag cacgtagctg cgaatctggt tgccccaggc
     2041 gatatcgccc ttttcgctgt acatcttggc cagctcggca tcgcgtttgg cctgctcaat
     2101 ctgctccaat ttggacttca ggatcgatag cgccatgcgc ttgttctgcg cctggctgcg
     2161 ctcgttcacg cattccacca tgatgccggt cggcttgtgc cgaatccgaa cggcggtcgc
     2221 gaccttgttg acgttctgcc cgcccgctcc ggacgcgcgc cggaaatact cgatgtccag
     2281 atcgccctcg ggcagctcga tcttcgtctc ttcgatctcc ggcagcacgt ccacgccgac
     2341 gaacgacgtc tgccgcttgc cctgagcgtt gaacggactc agccgcacca ggcggtgaac
     2401 gcccgcctcg cacgacagat agccatacgc gtacggcccc tcgacgcgca gcgtgaccga
     2461 ctgaatgccg gcttcctcgc cgtcgcggcg gtcgacctcg gcaacgtcgt agccgttctt
     2521 ctcaaagtac atcacgtaca tgcgcagcag catctcggcg aagtcgcacg agtcgacgcc
     2581 gccggcgccg gcttgaatgc tcaggaagca cggccgggcg tcattcggcc cggatagcag
     2641 cgtcagcagt tcgacctggt cggtcttggc ggccaactgc tccaactcgc gctggacctc
     2701 ggcttccgcg tccgcgtcgc tctgctcgcg ggccagctcc agcagcacgc ccgcgtcctc
     2761 ggacttggcg agcagttcgc gaaccgggtc cacgacggcg cgcagtccct tgagctcctg
     2821 gaccaccttc tgggcgtttt cctggatatc ccagaagccc ggctgggcca tgcgcttggt
     2881 cagctcgtcg atgcgtgcgg actttgcggg caggtcaaag agagtcccgg atcgcgtgga
     2941 tacgggtcgt caggtcgtgc agtacagccg ctgggtcttc gaactgtacg gttgccatga
     3001 tcgcgctaat tatagctctt cgcgccctgg ttgtcagtcg gggcggggat aagggtgata
     3061 aggtgacaaa gtgatagggt gataaggggg cgctgcgcgg agagacggga gaggagtgag
     3121 gagaaagggg acgggcgcga acgattcgcg cgagcggctc cgcgcggcgt taccccaggt
     3181 gatcaagcgg tctgataaaa aggcgtgtgg ctccgcgttt tggggg
//
LOCUS       DAOSYS010000336         3215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23697_length_3215_cov_4.654747, whole genome shotgun sequence.
ACCESSION   DAOSYS010000336 DAOSYS010000000
VERSION     DAOSYS010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23697_length_3215_cov_4.654747"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLU99_14885"
     CDS             complement(<1..255)
                     /locus_tag="PLU99_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18377.1"
                     /translation="MGERRREERHAAEYVLPQTVGVTPGIGPPPPTAYVATGMDSGIS
                     ARPASPRGRRHWIDDDPVAELAELAASAGALVVGRVIQRRD"
     gene            complement(576..>3215)
                     /gene="topA"
                     /locus_tag="PLU99_14890"
     CDS             complement(576..>3215)
                     /gene="topA"
                     /locus_tag="PLU99_14890"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOO18378.1"
                     /translation="VNAQQARRILDRIVGYELSPLLWNKIARGLSAGRVQSVAVRLIV
                     EREREIRAFVPEESWKIAAFLTPDQGGLGELRQNWIEFVKGERSQKDVQAWLSERGCF
                     RTELVEIDGKPFKPARVDEARAVAEALGLRVSRVERHPWAEYEHLKLEQVAIRGELAT
                     GEAPPFTLAELKSRRATTRPPAPFTTATLQQTASTVLRFSASRTMRTAQALYEGIDLN
                     GEGAVGLITYMRTDSTNLAAEAVDQARSFIKQSYGQNYVPESPNVFGKRQARAQEAHE
                     AIRPTDPRRTPESVRGALNPEQFKLYELIWKRFIACQMSPAEWDATSVSVTCDTRLGL
                     ARFAGSGRKLVFDGFMRVAGVTSEDQILPLMSPGQPIGLLGLEPKQSYTSPPPRYTEA
                     SLVKAMEAEGIGRPSTYATIIDTIQERGYVEQVDRKFVPTSLGELVTDKLVEHFPKIM
                     DVKFTSYMEDELDKIEDAHLDWVQVLHEFYDPFRELLSKAAEEMQAARSQPSEHVCPN
                     CNAPMVYRWSRTGRFLACSNYPKCKATMNVDRAGHPLVPETTDQVCELCGQPMLLRQS
                     KNGFFLGCGGYPECRNTIPCDEQGKPLRLVTEKELERPCDACGEGTMVVKKKGLRRFL
                     GCNRFPECKNLAPLPADVRLERKPAPPPEEAGVKCDKCGRPMVIRSGRRGKFVACSGY
                     PRCRNTKPVERLEALKAAAAASGDNGMTPNSPGGAVDSLASGSMPERQAGPATGDPHP
                     QFGTPPPGFAWTRTGRPVVEVMPAPGTLHCPQCGSTMELKRSRFGPFFSCSNYPKCKF
                     NANLRGEAKKEGEELMPAPARPKALRTDIPCPECGAPLVVRVGRRGKFLGCSNYPKCR
                     TAQELPPGFDLGAVTAAAAAGNE"
BASE COUNT          537 a         1121 c          953 g          604 t
ORIGIN      
        1 atcccgccgc tgtatcaccc gtcccaccac gagcgccccc gcggaggccg ccagttccgc
       61 cagctccgcc accgggtcgt cgtctatcca atgccgccgg ccgcgcggcg atgccggccg
      121 tgcgctgatg ccagaatcca tccccgtcgc gacgtaggcc gtcggcggag gcggtccgat
      181 ccccggcgtc acgcccacag tctgaggcaa aacgtactcg gcggcgtgcc gctcctcgcg
      241 gcgccgctcc cccaccagaa cgccaaccag cagcgcccgc tcctggatga ccgaaagctc
      301 ctcgcgcgcc gcactgacga ttggcgagct atttccacgc tcgccacccg cactacggcg
      361 tccccacggc ttgtgcttgt tcttcagatg cagactccta tttcacgcaa agcgggcaaa
      421 ccaacgtgct gggccggcgg cccggcccgc gctttgcgac accattaata gtctagcatg
      481 ccgcccccgc cggacgaagc caaaatgaag cccatcttga tgcggttccc acccaaaccg
      541 ccgcgacgac gcagggtgcc ggggccgaaa aacttttatt cgttgccggc cgccgctgcc
      601 gcggttacgg cgcccaagtc gaagcccggc ggcaattcct gggcagtccg gcacttggga
      661 taattgctgc agccgaggaa cttgccccgc cgcccaacgc ggaccaccag cggggcgccg
      721 cactctggac aagggatgtc ggtgcgcagg gccttcggcc gggcgggcgc cggcatgagt
      781 tcctcgcctt ccttcttcgc ctcaccgcgc aggttggcgt tgaacttgca cttgggatag
      841 ttgctgcatg agaagaacgg gccaaaccgg ctgcgcttca gctccattgt gctgccgcat
      901 tgcgggcagt gcagtgtgcc cggcgcgggc attacttcca ctaccggccg gccggtgcgc
      961 gtccaggcga acccgggcgg cggcgtgccg aactgcgggt gcggatcgcc cgtcgccggc
     1021 cccgcttgcc tctcgggcat cgaccccgag gccaaactat ctacagcacc gccgggcgag
     1081 ttcggcgtca tgccgttgtc tccgctggcc gcggctgcgg ctttcagcgc ttcgaggcgt
     1141 tcgacgggct tcgtattgcg gcagcgcgga taacccgagc acgccacaaa cttgccgcgc
     1201 cgtcccgagc gaatgaccat cggccgcccg cacttgtcgc acttgacgcc cgcctcttca
     1261 ggcggcggag ccggttttcg ttccagccgc acgtcagcgg gcagcggggc caggttcttg
     1321 cactcgggga agcgattgca gcccaggaag cggcgcaagc cctttttctt gacgaccatt
     1381 gtgccctccc cgcacgcgtc gcagggccgc tccagttcct tctctgtgac taagcgcagc
     1441 ggcttgccct gctcatcgca ggggatggtg ttgcggcatt cgggatagcc gccgcagccc
     1501 aggaagaatc cattcttgga ctgccgcagc agcattggct ggccgcacaa ctcgcatacc
     1561 tggtcggtcg tttccggcac cagcgggtgt ccggcgcggt ccacgttcat ggtcgctttg
     1621 cacttggggt aattggagca ggccagaaac cgtcccgtcc ggctccagcg atacaccatc
     1681 ggcgcattgc agttcgggca gacgtgctca cttggctggc tgcgcgccgc ctgcatttcc
     1741 tcagccgctt tgctgagcaa ctcgcggaat ggatcgtaga attcgtgcag cacctgtacc
     1801 cagtccaggt gcgcatcttc gatcttgtcc agctcatcct ccatgtagga ggtgaacttg
     1861 acatccataa tctttggaaa atgctcgacc agcttgtcag tcacaagttc gcccagcgaa
     1921 gtcgggacga acttgcggtc tacctgctcg acatagccac gctcttgaat cgtgtcgata
     1981 atcgtggcat atgtgctggg ccggccaata ccttccgctt ccattgcttt aaccagcgaa
     2041 gcttcagtgt agcgcggcgg cggcgacgtg tagctttgct tcggctccag gcccaatagg
     2101 ccgatcggct ggcctggcga catgagcggc agaatctgat cctcgctggt cacgcccgca
     2161 acgcgcatga atccgtcgaa caccagcttc cgccccgaac ccgcgaaccg cgccagcccc
     2221 aaccgcgtgt cacaggtcac agacacggac gtagcgtccc actcggccgg cgacatctgg
     2281 cacgcgataa agcgcttcca aatcagttcg taaagcttga actgctcggg attgagagcg
     2341 ccgcgcacgc tttccggtgt gcgccgcgga tccgtgggcc ggatcgcctc gtgagcctct
     2401 tgcgcgcgag cctgccgttt gccgaaaacg ttcgggcttt ccggcacgta gttctgaccg
     2461 taagactgtt tgatgaaaga tcgcgcctgg tcgaccgcct cggccgcgag gttggttgaa
     2521 tccgtacgca tgtacgtaat caagccgacc gcgccctcgc cgttcaaatc gatgccttcg
     2581 tacagcgcct gggccgttcg catcgtgcgc gaagccgaga accgcagaac ggtgctcgcg
     2641 gtctgttgca gcgtggccgt agtgaacggg gccggtgggc gcgtcgtggc acgccggctc
     2701 ttcaactcgg ccaacgtgaa cggcggggcc tcgcccgtcg ccaactcgcc gcggatcgcg
     2761 acctgctcga gcttcaagtg ctcatattca gcccatgggt gccgctcgac gcggctaacc
     2821 cgcagcccga gcgcctcagc aacggcacgc gcttcgtcca ctcttgccgg cttaaacggt
     2881 ttcccgtcaa tctcgaccag ttcggtcctg aagcacccgc gctcgctcag ccaggcctgc
     2941 acgtccttct gcgaacgctc gcctttgacg aattcaatcc agttctgtcg aagctctccc
     3001 agcccaccct gatccggcgt cagaaaggcg gcaattttcc agctctcttc cggcacgaac
     3061 gcgcggattt cgcgctcgcg ctccacgatc agacgtaccg cgaccgattg gacgcgtccg
     3121 gccgaaagcc ctctcgctat tttgttccat agcagcgggc tgagctcgta tccgacgata
     3181 cggtccaaaa tgcgacgcgc ctgctgcgcg ttgac
//
LOCUS       DAOSYS010000337         3192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23905_length_3192_cov_5.305387, whole genome shotgun sequence.
ACCESSION   DAOSYS010000337 DAOSYS010000000
VERSION     DAOSYS010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23905_length_3192_cov_5.305387"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..2212)
                     /locus_tag="PLU99_14895"
     CDS             complement(245..2212)
                     /locus_tag="PLU99_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18379.1"
                     /translation="MARRDGMTVRQTVRDKTGSGGARQAAGWGSVQAAVLLLLAGFAV
                     YGNALTHPFLIDDHLLIENDPRISGFDLPGIFGREYWPSAGGNQLYRPLTLMSLAVSH
                     AICEAAWSFRVPNLLLHVAAGLAVYHLAMTLAPGRRWVALLAGLLFVVHPINSMALNQ
                     VVDRADLAAAAGSLWAAWLYLTDRGGWRRPVLAALLFILALLSKENAATLLGVFVVLD
                     LYLRGRELRATRRAWWRERGLRYYLPAAVVLGAYLLLRFNVLGGLVQDAERISPLDNI
                     IARPTYGLESGDSVFLARWGTPVAILGRAAGLLVWPHPLSWDYSYAETSAVHGWGEPR
                     LWVGVAVLSAGAVAAVVSWFGQRRVLVALGLMLATYSVVSNTFVVIASAFAERYLYLP
                     SAGFCLLVAFAVSALPEMWRGAAGPPRGLRVGVSCAAGAVLILLAVLTIHRNRDFVSE
                     MVLNSVDIETHPRAARMWSALAGEQLKAGNNRAAVVSAQKALEIYPPYPDAWRTAGLA
                     LFRLNEPDQALACLRESFKTGGQDHETANVHVAKILTQRGEYREAISLLRDFVQRNPQ
                     AAAPRNNLAWYLLTAEPAELRDLPAALQYAREAVSLEPQAGDFVDTYVAVLEGLGRHA
                     EAQRVIRDALIHIPADDPARPDLLRRLQQEP"
     gene            2343..2741
                     /locus_tag="PLU99_14900"
     CDS             2343..2741
                     /locus_tag="PLU99_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18380.1"
                     /translation="MDELVTRESWPEFCERFTHQHHGWLATLEQQAVRSTAGGGMIGG
                     PGALLEFRAIRLAEEAGREVLCITATGQGGEDHTGIVYNPVRLTFKKADDGAHSGLRV
                     DGRNGERLVLRFRVPALPEALNGLMANSAK"
     gene            complement(2828..2995)
                     /locus_tag="PLU99_14905"
     CDS             complement(2828..2995)
                     /locus_tag="PLU99_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18381.1"
                     /translation="MPRKSWSKKDERMYEHIEQSQRKEGRSKGRAKQIAAATVNKRRK
                     QEGRTKQQKSK"
BASE COUNT          612 a         1080 c          956 g          544 t
ORIGIN      
        1 ccagcggcag cgcgccactg gttaccgctt ggccgctctt cgcgagcttc tcgacctcgg
       61 cgcgcacctg cgccagatct actccgaggt cccgcagcac attaaccgcc gcgccgcagc
      121 cctatttgat cagccccagc agaatgtgct ccgtgtcgac gcattcctga tggagcgtaa
      181 gcgccacctg gttagcgagc accattacgt tgtagcctcg atccgtaagt cgttcgacca
      241 tgattcatgg ctcctgctgc aatctgcgca acaaatccgg acgtgcgggg tcgtccgcgg
      301 ggatatgaat aagggcgtct ctgatgacac gctgtgcttc cgcatgccgc cctaagcctt
      361 ccagcacggc cacgtaggtg tctacgaaat cgccggcctg aggctcgagg gaaaccgctt
      421 cccgggcgta ctgcaacgcg gcaggcaagt ctcgcaactc cgccggctcg gcggtcaaca
      481 agtaccaggc caaattattg cgcggcgcgg cggcctgcgg gttccgctgc acgaaatcgc
      541 gcagcagcga aatcgcctcg cggtattcgc cgcgctgagt gagaatcttg gctacgtgca
      601 cgttggccgt ctcgtggtcc tgtccaccgg tcttgaagga ctctcgcagg caggcgagcg
      661 cctggtccgg ctcgttcaga cgaaacagcg ccagcccagc cgtgcgccag gcatccgggt
      721 acggtgggta tatctccagg gctttttgcg cggatacaac tgccgcccgg ttattgcccg
      781 ccttcagttg ctcgcccgcc agcgcggacc acatgcgggc cgcacgcggg tgtgtttcta
      841 tatcaaccga gttcagaacc atctcggaaa cgaaatcgcg attgcggtga atcgtcagaa
      901 cagccagcag gatcagcacg gcgccggcgg cacagcttac gccgacgcgc aggcccctcg
      961 gcggtccggc cgcaccgcgc cacatctcag gcagcgcact caccgcaaat gccacgagca
     1021 ggcagaatcc cgcactgggc aaatataagt agcgctccgc aaatgccgac gcgatcacga
     1081 caaacgtatt ggaaaccacg ctgtacgtcg ccagcatcag ccccagagca accagtaccc
     1141 gccgctgccc gaaccacgaa accacggccg ccaccgcacc tgccgaaagc acagccacgc
     1201 ccacccacag ccgcggctcg ccccacccgt gaaccgcgct cgtctcagcg tatgagtagt
     1261 cccacgacag tggatgcggc cagaccaaca gaccagccgc ccgccccaaa atcgctaccg
     1321 gcgttcccca acgcgccaag aaaacgctgt cgccggactc caagccgtaa gtcggccggg
     1381 cgatgatgtt gtccagcgga ctgattcgtt ccgcgtcctg aaccagtccg cctagcacgt
     1441 tgaaccgcag tagcaagtac gcgcccaata cgaccgccgc cggcaggtaa taccgcaggc
     1501 cgcgctcgcg ccaccatgcc cgccgcgtag cgcgcagctc gcgcccgcgc aaataaaggt
     1561 ccagcacgac gaacacgccc agcaacgtcg cggcgttctc cttgcttagc agagccaaaa
     1621 taaacagcag cgccgccagg accggccgtc gccagccccc acggtccgtc aggtaaagcc
     1681 atgccgccca cagcgacccc gccgcggccg ccagatccgc gcggtccacg acctgattca
     1741 gagccatcga gtttatggga tggaccacaa acagcagacc ggccaacagt gccacccagc
     1801 gccgcccggg cgcgagcgtc atcgccaggt gataaaccgc cagccccgcg gccacgtgta
     1861 gcagcagatt cggtactcgg aagctccagg ccgcttcgca gatggcgtgg ctgacggcca
     1921 aactcatgag cgtcagcggt ctatacagtt ggttgccgcc ggcgctcggc cagtattccc
     1981 ggccgaagat cccgggcaag tcgaagccac tgatacgcgg atcattctcg atcagcaggt
     2041 gatcgtcgat caggaaggga tgggtcagag cgtttccgta gaccgcgaaa ccggccagca
     2101 gcaacaaaac cgccgcctgc acgctgcccc agccggcagc ctgcctggca ccgcccgaac
     2161 ctgtcttgtc ccgcaccgtc tgccgcacgg tcatgccatc gcgtctcgcc atgggcgcct
     2221 tttcttcgaa ttcacaccgg tttcagcgcc cattcacgcg gctcaggtac acattaaacg
     2281 aaaacgaccg aggttgcgcg ccgaccggcg agttgccgag acttttggtt cggagtctga
     2341 agatggacga gcttgttacg cgcgaaagct ggccggagtt ctgcgaacgc ttcacgcatc
     2401 agcatcacgg ctggctggcg acgctcgaac aacaggcggt gcgctccacc gcgggcggcg
     2461 gcatgattgg cgggccgggg gcgcttctgg agttccgcgc tatacgactg gcagaagagg
     2521 ccggccggga ggtgctttgc atcactgcta ccggccaggg aggcgaggac cacactggta
     2581 ttgtgtacaa ccccgtgcgt ctgacattca agaaggctga cgacggggcc cacagcgggt
     2641 tgcgggtgga cggtcgaaat ggcgagaggc tggtcttgcg tttccgcgtg ccggcgttac
     2701 ccgaggctct gaatgggctg atggcgaaca gtgcaaagtg aatggcgggg gcccatggtg
     2761 ccggagggtg ccgttctgac cgcgacacgc actgcttgaa ggtgccgctt cacggagtgg
     2821 cacacactta cttcgatttc tgctgcttgg tgcggccctc ttgtttgcgg cgcttgttca
     2881 ccgtcgccgc ggcgatttgc tttgcccggc ctttcgaacg gccctccttg cgctggctct
     2941 gctctatgtg ctcgtacata cgttcgtctt ttttgctcca ggatttccgt ggcatgagag
     3001 gtgctccagc cgtgtggccc gcatccggga tgctagcgcg gtgctaacgc ctgatgcgtt
     3061 gaattcgccg tgatccacgc acaaatcccc gcggggcgtc gcataccgcc tcgcggggac
     3121 cgcactccag agagagcaag gaggagagaa agcttgtatc gtccggccgg cgggtcccac
     3181 gcgccgctct gg
//
LOCUS       DAOSYS010000338         3186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_23959_length_3186_cov_5.562440, whole genome shotgun sequence.
ACCESSION   DAOSYS010000338 DAOSYS010000000
VERSION     DAOSYS010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23959_length_3186_cov_5.562440"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PLU99_14910"
     CDS             complement(<1..1756)
                     /locus_tag="PLU99_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOO18382.1"
                     /translation="MNALDRLIGVFAPSVALRRVGARAALQRAASRDGFVRAYEGAKT
                     GRRTGGWITGSTSADAEVGGSAVKLRDRARALCRDNPYASRARDVFVANVVGTGITVK
                     AEDARHAWARWVNECDADGLLDFYGLQALVMRCLFESGECLIRYRERRPEDGLIVPLQ
                     LQVLEPDYLDSGKTGPLKGGGWMVSGVEYDAIGRRTAYWLFNQHPGDTVNRGVGLESK
                     RVPAEQVLHIFERQRPGQTRGVPRMASILLKMRDLDDYEEAELVRKGIESCFSAIVTT
                     EDPAVGLTEGTTDAAGNRIESLGAGLIQYLKPGQDVRFGSPASSGDYGNYTKTQLRAI
                     ASGIGITYEQMTGDLTEVNYSSIRAGLVEFYKTVDMLQWHVLVPMMLQPIWSRWADTA
                     FAVKAVAKRPPARPTWTPPRRQWVDPAKEVNAARDEISANLTSVSETIRARGNDPDRV
                     FEEIAAERKLMAALGITSDLLPPAPAPAAPPDPASRELTLALVRSLAQEPKSGDTVIH
                     NHPPAVTIGATEVRAEIAVPPATVVNEIHEREQPAPVVQVVNEISEREQPAPVVNVAA
                     PVVNVAAPNVAITNDVQPA"
     gene            complement(1753..1959)
                     /locus_tag="PLU99_14915"
     CDS             complement(1753..1959)
                     /locus_tag="PLU99_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008036057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18383.1"
                     /translation="MAYTTTHLAAIEAAIATGELTVTVDGRSVTYRSVSDLLKAKRDI
                     EAGLIASGTTERPVSQSYVQRVRT"
     gene            complement(2429..2677)
                     /locus_tag="PLU99_14920"
     CDS             complement(2429..2677)
                     /locus_tag="PLU99_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18384.1"
                     /translation="MPEDDLIASILDTVATHVPISKPKRDMIDADLRLNWGGAEIYIP
                     KHSPTRRQAIRDATGTYAEIAAAFNVSLTTVWRVRKGR"
     gene            complement(2664..>3186)
                     /locus_tag="PLU99_14925"
     CDS             complement(2664..>3186)
                     /locus_tag="PLU99_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOO18385.1"
                     /translation="QVYAFVARRRWCFATKGVFGMGRPLVEDEKKRRQRLRVKRKRGV
                     PVEPIGVDGGKGMLYARLKQMEPGPGYLHFPQEPAFDDEYFAQLAAEKLVTRFKGHRP
                     IQEWVQTRARNEALDCLILALVALRLSGATLKDATVAVDAEKDQKAEPSTPLPPPTRG
                     NDLLERIRSRARR"
BASE COUNT          483 a          960 c         1099 g          644 t
ORIGIN      
        1 cggcgggctg cacatcgttg gtaatcgcca cgttcggcgc ggcgacgttg accacaggcg
       61 cagcgacgtt gaccacaggg gccggctgct cgcgctcgga gatctcgttg actacctgga
      121 cgacgggggc aggctgctcg cgctcgtgga tttcgttgac cacggttgcc ggcggcacgg
      181 cgatctctgc gcgcacttcg gtggcgccga tggtgacggc gggcgggtgg ttgtggatga
      241 cggtatctcc ggacttcggt tcctgcgcca gcgatcgcac cagggcgagg gtcagttccc
      301 tgctggctgg atcaggcggc gcggccggcg cgggcgctgg cggcagcagg tcgcttgtga
      361 ttcccagggc ggccatcagt ttgcgctcgg cggcgatttc ctcgaaaacc cggtccgggt
      421 cgttgccgcg ggcgcggatg gtctccgaaa cgctggtcag gttggcggag atttcgtcgc
      481 gggccgcgtt gacttccttg gctggatcga cccattgccg gcgcggcggc gtccacgtcg
      541 ggcgggcagg cgggcgcttg gccacggcct tgacggcgaa ggcggtgtcg gcccagcgcg
      601 accagatcgg ctggagcatc atcggcacca ggacgtgcca ttgcagcatg tcgaccgttt
      661 tgtagaactc gacgagcccg gcgcggatgc tggagtagtt gacttcggta aggtcgccgg
      721 tcatctgctc ataggtgata ccgatgccgg aggcaatggc gcgaagctgc gtctttgtat
      781 agttcccgta atcgccgctg ctggccggtg aaccaaagcg cacatcctgt ccgggcttga
      841 ggtactggat gaggccggcg ccgaggcttt cgatgcgatt tccggcggcg tccgtcgtgc
      901 cttccgtgag gccgaccgcc gggtcttcgg tggtgacgat ggcggagaag caggattcga
      961 ttcccttgcg gaccagttcg gcctcttcgt aatcatcgag gtcacgcatc ttgagcagga
     1021 tcgaggccat gcgcgggacg ccgcgcgtct gcccgggtcg ctggcgctcg aagatgtgca
     1081 gtacctgctc ggccgggact cgcttcgatt ccaggccgac gccgcgattg acggtatctc
     1141 cggggtgctg gttgaacagc caataggcgg tgcggcggcc gatggcgtcg tattcgacgc
     1201 cggagaccat ccagccgcca cctttgagcg gtccggtttt tccggaatcc aggtaatccg
     1261 gctcgagaac ctgcaactgc agcgggacaa tcaggccatc ttccgggcgc cgctcgcggt
     1321 agcggatcag gcattcgccg gactcgaaca ggcatcgcat gaccagcgcc tgcaggccgt
     1381 agaagtcaag cagcccgtcg gcgtcgcatt catttaccca gcgtgcccag gcatggcgcg
     1441 cgtcttcggc tttgacggtg attccggtgc cgacgacgtt ggccacgaac acgtcgcgcg
     1501 cgcggcttgc atagggattg tcacggcata gggcgcgggc gcggtcgcgc agcttgacgg
     1561 cgctgccgcc gacttcggca tcggccgagg tgctgccggt gatccagccg ccggtacggc
     1621 gtccggtctt cgcaccttca taggcacgga cgaagccgtc gcgcgacgcg gcgcgctgga
     1681 gcgcggcacg ggcgccgacg cggcgcagtg caaccgatgg ggcgaagacg ccgatcaggc
     1741 ggtcaagcgc gttcacgtcc tcacccgctg cacgtagctt tgcgaaacgg gccgctccgt
     1801 tgtgccagac gcaatcagcc ctgcctcgat gtcgcgtttg gcctttagga ggtcgctgac
     1861 cgagcgatag gtgacgctgc ggccgtcgac cgtgaccgtc agttcgccgg tggcgatggc
     1921 ggcctcgatc gccgccaggt gcgtggttgt gtatgccatg tggccgttgc tttccggtaa
     1981 tgcccaccat cagcatgcgc tttggcatca ttgcaaaact acggaaaaat gaaattcacc
     2041 tcccatttct ggcggtttgg cggtcgattt tgtgaacagg atatgcggcg gttttgcagc
     2101 gttaaataga ataggcggcg tttgtgccgt ctacttctag ttagtggcct tcgtgtttcg
     2161 tctccgcgca ctcggcgcga ctcgcaagcc cgctgttgaa aattctcgtt gcgtagcctt
     2221 tcgcggcgca ctgctcaaca atcagcttca gttgagcatc gctgatgggc gcaggcgtac
     2281 aggctgtgag cgagagcaac agcatcgctg ccgtcgtatt gaccttgtgc atttcattcc
     2341 tttcgggggc gctcggcccc taacaccacg gtcgaggtcg cgccttcggc gctggacgct
     2401 tcgccggcag gccgcacaac ttggtcgttc accgcccctt ccgcacacgc cagacggtcg
     2461 tcaaactgac attgaatgcc gcggcgattt cggcataggt gccggtggcg tcgcggatgg
     2521 cctggcggcg ggtcggagaa tgcttgggga tgtagatctc tgcgccgccc cagttcaggc
     2581 gcaggtcggc gtctatcatg tcgcgcttcg gtttgctgat cggcacgtgc gttgcaacgg
     2641 tgtcgagtat ggaagcgatt aagtcatctt ctggcacggg agcgaatcct ttctagaagg
     2701 tcattgcctc gggttggcgg cgggagcggg gttgagggtt ctgctttctg gtctttttcg
     2761 gcgtcgaccg cgacagttgc atccttgagc gtggcgccgg agaggcgcag cgcgaccagc
     2821 gccaggataa ggcagtcgag cgcctcgttg cgggcgcggg tctgcaccca ttcctgtatc
     2881 ggcctgtggc ccttgaagcg tgtgaccagc ttttcggcgg ccagctgggc gaagtattcg
     2941 tcgtcaaagg cgggttcctg cgggaagtgc aggtagcctg ggccgggttc catctgcttc
     3001 aagcgcgcgt agagcattcc cttgccgccg tcgacgccaa tcggctcgac cggaacgccg
     3061 cgtttgcgct tgacgcgcag gcgctggcgg cgcttcttct cgtcctcgac cagcgggcgg
     3121 cccattccga agacgccctt ggtggcgaag caccagcgcc ggcgggcgac gaaggcatag
     3181 acctgg
//
LOCUS       DAOSYS010000339         3147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_24295_length_3147_cov_4.651358, whole genome shotgun sequence.
ACCESSION   DAOSYS010000339 DAOSYS010000000
VERSION     DAOSYS010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24295_length_3147_cov_4.651358"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PLU99_14930"
     CDS             <1..652
                     /locus_tag="PLU99_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18386.1"
                     /translation="GDAAAAAQRRVEHITAAELPPASLLSRAARRGRRAVSHWLWRRR
                     LGALGPKSYIDWPAYVVGGSSIAVGAQVAVWHHARLEAFNAQPGVIRISIGDLTSVQP
                     YAHIGAAESVRIGRGVSIASSVYISDHDHDWCDPGEPAILNERLRTAAVEIGDFVWLG
                     ERAIVLKGVTIGAYSVIGAGSIVTRDVPPYTLAVGAPARVIRRYDHERRLWTPVKQ"
     gene            649..2070
                     /locus_tag="PLU99_14935"
     CDS             649..2070
                     /locus_tag="PLU99_14935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOO18387.1"
                     /translation="MKRALIISYFFPPDGGAGTQRAAKFCKYLPEHGWEATVVTRDPA
                     ATRDRMVPADAALLRDIGRDTRVVRVAEEARLSPWAAALPRIDVGYTWLEPALAAAKA
                     QIASWGPDVVLITMSPFSLAFLGWRLQQEAGVPVVYDLRDPWALDGWRLHGTRRRWRR
                     DLAVMKDTLKAADGVIANTPDAAQAIAKAVPSLSPQRLTCITNGYDAEDFASPEPARP
                     DPREAPHFRLVHTGTLLTDCLYVYCGPLGWLKRLKHYRPEPIDTSGRTPFHLLRAIAI
                     LRARGHPCGSRVRVQLVGGDDPQTQRCVRESPAPDCVELTGYVSHPESVRYARQADAL
                     FLPLHGLPPGYRSLIVPGKTYEYLATRRPILGCLPPGHARDLVERSGRGFCAEPCDAA
                     NIAHALADLFDTWRSGRLKAAEDADFLNRLERRTLTRHLAAFLDQLAPAKAEPASDHS
                     PAVIAHHAPGHLPESRSVSCNRT"
     gene            2055..>3147
                     /locus_tag="PLU99_14940"
     CDS             2055..>3147
                     /locus_tag="PLU99_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18388.1"
                     /translation="MQPNIGIAGSNLEYAGQTLLHPVGLVATLVLGVLMLILPRRTAV
                     LPMIAMACFLAPVQRLVILGLDLNLLRILVLFGWMRLLVRGELHGFRWKLIDKVVLAW
                     VVAGACIYTIQQGTSQALINRLGNAYDALGMYFLFRCLVRTWDDIARTAMGFAVISVP
                     VAVAFLVENQTGYNVFSTLGGVEESTLVREGRIRCQGAFSHPILAGCFWGSIMPLIAS
                     LWWNDRRQRVWVVIGLGCAAIIVLCCASSTPVISVVIGAMAAALYPFRRHLRTIRWTA
                     LIGLVCLHVLMRAPVWHLLSRVQAVGGSTGWHRYHLIDQSIARVGEWWLWGTPSTAHW
                     GLGLRDVTNQYVLEGVRGGLLTLVLFVILI"
BASE COUNT          494 a         1022 c         1032 g          599 t
ORIGIN      
        1 cggtgacgcc gcagccgctg cgcagcggcg cgtcgagcac attacggcgg cagagctccc
       61 gccggcgagc ctgctgagcc gggccgcgcg gcgtggaagg cgggccgtgt cgcactggct
      121 gtggcgccgc cgcttaggcg cgctcgggcc gaaatcctat atcgactggc cggcgtacgt
      181 ggtgggcggc agttcaattg cagtcggcgc ccaggtggcg gtgtggcatc atgcgcggtt
      241 ggaagccttc aatgcacagc ctggcgtaat ccgcatttca atcggcgatc tcacatccgt
      301 tcagccctat gcacacattg gcgccgccga aagcgtgcgc atcgggcgcg gcgtgtccat
      361 agcctcatcc gtctacatca gcgatcacga ccacgactgg tgcgatcccg gcgaaccggc
      421 cattcttaat gagcgcctgc gcaccgccgc cgtagaaatc ggcgatttcg tctggctcgg
      481 agaacgcgcc atagtgctca agggagtgac catcggcgca tatagcgtga tcggcgccgg
      541 cagcatcgtg acgcgcgacg tgccgcccta cacgctggcg gtcggcgccc ccgcccgcgt
      601 gattcgccgc tacgaccatg agcgtcgcct gtggaccccg gtgaagcaat gaaacgcgca
      661 ctcatcatca gttacttctt tccgcccgac ggaggcgccg gaacccaacg cgccgccaaa
      721 ttctgcaagt atttgccgga acatggctgg gaagcgactg tcgttacgcg tgatccggcc
      781 gcgacccgcg atcgcatggt gcctgcggac gcggccttgt tgcgcgacat cgggcgagac
      841 acacgcgtgg tccgcgtggc ggaagaggcg cgcctcagcc catgggcggc cgctctgccg
      901 cggatcgacg tgggctatac ctggctcgaa ccagccctgg ccgcggccaa ggcgcagata
      961 gcgagctggg ggccggacgt ggtgctgatc acgatgtctc cgttctcgct ggcgtttctg
     1021 gggtggagat tgcagcagga ggccggcgtc ccggtggtgt acgacctgcg tgatccgtgg
     1081 gcgctcgacg gctggcgcct gcatggaaca cggcggcgct ggcggcggga tctggcggtc
     1141 atgaaggaca cgctcaaggc cgccgatggc gtcattgcca acacgcccga cgcggcgcag
     1201 gccatcgcca aggccgtgcc ctccctgtcg ccgcagcgcc ttacctgcat tactaatggc
     1261 tatgatgctg aggactttgc ctcgccggaa ccggcccgcc ccgatccgcg cgaagcccca
     1321 catttccggc tcgtgcacac tggtacactg ctcacggact gtctgtatgt ctactgcggg
     1381 ccgctcggat ggctcaagcg gctgaagcat taccgtccgg aaccgatcga tacgtccggc
     1441 cgcacaccgt tccacctgtt gagagccata gcaatactgc gcgcccgcgg tcatccgtgc
     1501 gggagtcgcg tccgcgtgca gctcgtcggc ggcgatgacc cgcagacgca gcggtgcgtg
     1561 cgcgagtcgc cggccccgga ctgcgtcgag ctcaccggct acgtttcaca ccccgaatcg
     1621 gtgcggtacg cccggcaggc cgatgcgctg ttcttgccgt tgcacgggtt gcccccgggc
     1681 taccgcagct tgatcgtccc cggaaagacc tacgaatacc tggccacgcg ccgcccaata
     1741 ctcggctgcc tgccgcccgg ccacgcgcgc gacctggtcg agcgctcggg ccgcgggttc
     1801 tgtgccgagc cgtgcgacgc agcgaacatc gcgcacgcgt tggccgactt gttcgacacc
     1861 tggcgcagcg ggcgcttgaa ggcggcggag gacgcggatt tcctaaaccg tctcgagcgg
     1921 cgcaccctga cccggcactt ggcggctttt ctggatcaac tggcgcccgc aaaagccgaa
     1981 cccgcatcgg accatagccc cgcggtcata gcccaccacg cacctggaca cctgccggag
     2041 agtcggagcg tctcatgcaa ccgaacatag gcattgccgg ctccaatctg gaatacgcgg
     2101 ggcaaacact actgcaccct gtgggcctgg tggcaacatt agtgttgggc gtgctaatgc
     2161 tgatcctgcc gcgccgcaca gccgtgttgc caatgatcgc catggcgtgt tttctcgcgc
     2221 ccgttcagcg cctggtcata ttgggcctgg atttgaacct gttgcgtata ctggtgctct
     2281 tcggctggat gcggctgctg gtgcgcggcg aactgcacgg ctttcgttgg aagttaatcg
     2341 acaaggtcgt gctggcgtgg gtggttgccg gtgcttgcat ctacactatc cagcagggga
     2401 ccagccaggc actcattaac cgtctcggta atgcctacga tgcgttgggg atgtactttc
     2461 tgttccgctg cctcgtgcgt acctgggatg acattgcgcg gacggcgatg gggttcgcgg
     2521 tcatcagcgt gccggtggcc gtggctttcc tggttgaaaa ccagaccggg tacaacgtgt
     2581 tctctaccct gggcggtgtt gaggaaagca ccttggtgcg tgaaggtcgt atacgctgcc
     2641 agggcgcatt ctcgcacccc attctggcgg gctgcttttg gggctcgata atgccgctga
     2701 tcgcatcgct gtggtggaat gaccgccggc agcgagtgtg ggtcgtgatc ggcttgggct
     2761 gcgccgcgat aatcgtcctg tgctgcgcat ccagtacacc cgtcatttcc gtggtaatcg
     2821 gcgccatggc cgccgcactc taccccttcc ggcgccacct gcgcaccatt cgctggacgg
     2881 cgctgatcgg gctcgtctgt ctgcacgtgc tgatgcgcgc cccggtctgg cacctgcttt
     2941 cgcgcgtcca agccgtgggc ggctccaccg gttggcatcg gtaccacctg attgatcagt
     3001 caattgcacg cgtgggtgag tggtggctgt ggggcacgcc gagcaccgcg cattggggcc
     3061 tcggcctgcg cgatgtcacc aaccagtatg tcctggaggg agtgcgcggc ggactgctga
     3121 cactagtgct cttcgtgatt ctgatag
//
LOCUS       DAOSYS010000340         3128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_24467_length_3128_cov_3.073869, whole genome shotgun sequence.
ACCESSION   DAOSYS010000340 DAOSYS010000000
VERSION     DAOSYS010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24467_length_3128_cov_3.073869"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..1402)
                     /locus_tag="PLU99_14945"
     CDS             complement(272..1402)
                     /locus_tag="PLU99_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOO18389.1"
                     /translation="MDSQQKLRIDSSDIKRILDMLDAHEKYTVGGQRATTPRYPYRVP
                     ALNVHLQQSDGAMVRHVLPSRSLGPDGISFLLNKFVYPQTPCRFDLITTHNNWHSVTG
                     FVTECTYIHGTGAVYDAHARFNQPIDIALFTTAALRVRVLLVDDSRTARGLLSQMLRG
                     CNADVVACDNGQQAIDLATKTPFDAVLMDIEMPVLNGYEATRQLRARNFVQPIVAVTG
                     LTSAEDRQRCFEAGCDEYLPKPADRNAIEELVNRLRPEPLMTTLDLENPELASLIDQY
                     VAEIQERAVQMQNCFAQGDLAGLTHHLRNLRSEAPGFGFEPIGRAAAEVEDQIAQGAL
                     PADIGLKLGRLARLCCAVRPLALQPTEPPLEPPAMVRVAAPA"
     gene            1744..>3128
                     /gene="malQ"
                     /locus_tag="PLU99_14950"
     CDS             1744..>3128
                     /gene="malQ"
                     /locus_tag="PLU99_14950"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOO18390.1"
                     /translation="MLKQRAAGVLLHPISLPGPHGCGDLGRPAHELVDFLASAKQRWW
                     QMLPIGPPGRGNSPYSAHSAFAGNPLLVSLELLAKEGLLQRRELTPATEFSAKRVVYP
                     AVWRYRMERLRWAFERFSAAATDQRAFRQFCRSQRHWLEDYALYAALRHIQGGRAWLD
                     WTEDLRLRRSRGVVKARAELARGIAFEKFVQFKFAQQWAALRAYARQRGVGLLGDMPI
                     FVAFDSADVWAHRELFRLDSVGRPLVVSGVPPDYFNRNGQLWGHPHYRWSRHAATGFA
                     WWVARFRQAFAHFDAVRVDHFLGFHRMWEVPARAATARHGRWIRTPGQALLQAVTGAL
                     GPVQIIAEDLGAVTPEALALRDEFGFPGMRVLQFAFGDDGPGSRYHQPHSYPRNCAVY
                     PGTHDNETAVGWFTNLRAAAKKKARRQSGRRVADTPYERVLRYTGTSGGDINWDLIRI
                     AAMSVADLAIVP"
BASE COUNT          517 a          957 c         1051 g          603 t
ORIGIN      
        1 tcaacctgcc cggtcgaaac cattcgcccc catgcacttg tcaaagatcg ccggcacgca
       61 cgctgagtgg cccacgaaac ccgccacaac gggccgcgtt cggcctccgc cgcatgccgc
      121 cacgtcgaaa ttatacccgg aaacgtaaca tttggcctgc ttctttagcc cctcgcacga
      181 gcgcgaagtc aaatctcgca ttttcgccgt agcgtggagg gaatccgagc gacgggcttt
      241 gccccgttga attgcgggca gccgtgcaac ctcaggcagg cgcggcgacg cgaaccatgg
      301 cgggcggctc cagcggcggc tcggtgggtt gcagggccag cggccgaacc gcgcaacata
      361 gccgcgccaa gcgcccaagc ttcaggccga tgtccgcggg cagagcgcct tgcgcgatct
      421 ggtcctcgac ttcggccgcg gcgcgtccga tcggctcgaa cccgaaaccg ggcgcctcgc
      481 tcctgaggtt acgcaggtgg tgggtgaggc ccgccagatc gccttgcgca aagcagttct
      541 gcatttgcac ggcgcgctcc tggatttcgg ctacatattg gtcgatcagc gatgccaact
      601 cgggattttc caggtccaat gtggtcatta gcggctcggg tctcaagcga ttgacaagtt
      661 cctcgatggc gttgcggtcg gcgggtttgg gcaagtattc gtcgcagccg gcctcgaagc
      721 agcgctggcg gtcctcggcg gaggtgaggc ccgtcacggc cacgatgggc tggacgaaat
      781 tcctggcccg cagttggcga gttgcctcgt acccgttgag taccggcatt tcgatgtcca
      841 tcaggacggc atcgaacggc gttttcgtcg ccaggtctat cgcctgctgc ccgttatcgc
      901 aggccacgac gtcagcgttg cagccgcgga gcatctgact cagcaggccg cgcgccgtgc
      961 gactgtcatc caccagcagc actcggacgc gcagtgcggc ggtggtaaag agcgcaatat
     1021 cgatgggctg gttgaagcgg gcgtgggcgt cgtatacggc gcccgtcccg tgaatgtaag
     1081 tgcattcggt gacgaacccg gtgaccgaat gccagttgtt atgagtcgtg atcaagtcga
     1141 agcggcaagg cgtttgcgga tacacgaatt tgttgagcag gaaggagatt ccatccgggc
     1201 cgagactgcg gctgggcaga acgtggcgca ccatggcgcc gtcgctctgc tgcagatgga
     1261 cgttcagcgc ggggacgcgg taggggtagc gcggtgtggt tgcacgctgg ccgccaaccg
     1321 tgtacttttc gtgtgcatcg agcatatcga gaatgcgttt tatgtcagag ctgtcgatcc
     1381 gcagcttctg ttgcgaatcc atgcctgtcc ctctgatctg attccgcccc gggcctggct
     1441 ggcgcgcccc gcgagtttcg tggctgtgcg acggcagcct ggttgttgca gcgggcccac
     1501 gtgcggccca gccgacgatt cgaatagccg gttgggtccc agaaaactca cggatcacgc
     1561 cgtatcttgc gctccgggaa cggcccggtg cgcaactctt cttcggcgag ttaaagcggc
     1621 ttctgtatgc ggctttgcgc acaccggccg cggcccgcgt taagagacgt gatctgccca
     1681 gctctgtcca tttgcagtta cactatggcg atggcacgct cgagcaaacc aggaaccggt
     1741 ttcttgctga agcagcgcgc tgcgggcgtg ctgttgcacc cgatatcgct gccaggtcca
     1801 catggctgcg gcgatctcgg acgcccggcc cacgaactgg tcgattttct ggcgagcgcc
     1861 aagcagcgtt ggtggcagat gctgcctatc ggcccgcctg ggcggggtaa ctcaccatat
     1921 agcgcccatt cggcgtttgc gggcaatccg ctgctggtca gcttggagct gctggcgaag
     1981 gagggtctgc tacaacgccg ggaactgacg cccgcgaccg aattcagcgc gaagcgcgtg
     2041 gtgtatccgg cggtgtggcg ttatcggatg gaacggctgc gctgggcctt tgagcgtttt
     2101 tctgccgcgg cgacggacca gcgcgcgttc cggcagttct gtcgttctca gcggcattgg
     2161 ctggaggatt acgcccttta tgccgccctg cgacacatac agggcgggcg agcgtggttg
     2221 gactggacgg aagacctgcg gctgcgtcga agcagggggg tggttaaggc gcgggccgaa
     2281 ttggcgcgtg gaatcgcatt cgagaagttc gttcaattca agttcgctca acaatgggcg
     2341 gcgctgcggg cctacgcgcg gcaacgcggc gtggggctgt tgggcgacat gccaatcttc
     2401 gtcgcattcg attcggccga cgtgtgggcg catcgcgagc tattccgcct agacagcgtg
     2461 gggcggccgc tcgtggtctc aggcgtgccg ccggattatt tcaaccgcaa cggccagctt
     2521 tgggggcacc cgcattaccg ctggtcgcgc cacgcggcga cgggtttcgc ctggtgggtg
     2581 gcccggttcc gccaggcttt cgcgcatttc gacgctgttc gcgtagacca tttcctcggt
     2641 tttcaccgca tgtgggaagt ccccgcccgg gcggccaccg cccgccacgg acgctggatc
     2701 cgcacgcccg gccaggcgct gctccaagcg gtgaccggcg cgctcgggcc ggtgcagata
     2761 atcgcggaag atttgggagc ggtgacaccg gaggcgctgg cgctgcgcga cgagtttggc
     2821 tttccgggca tgcgggtact gcaattcgca ttcggcgacg atgggcccgg cagccgttat
     2881 caccagccgc actcttatcc ccgcaactgt gcggtctacc cgggcacgca cgataacgag
     2941 acggcggtgg gctggtttac aaatctgcgg gcggcggcca agaagaaggc gcgtcgccaa
     3001 agcggtaggc gcgttgcgga cacaccctac gagcgggtgc tacgctacac aggtacgagt
     3061 ggcggcgaca taaactggga cctgatccgc attgccgcga tgtccgtggc cgacctcgcg
     3121 atcgtgcc
//
LOCUS       DAOSYS010000341         3127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_24474_length_3127_cov_5.247396, whole genome shotgun sequence.
ACCESSION   DAOSYS010000341 DAOSYS010000000
VERSION     DAOSYS010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24474_length_3127_cov_5.247396"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1202)
                     /gene="dnaJ"
                     /locus_tag="PLU99_14955"
     CDS             complement(33..1202)
                     /gene="dnaJ"
                     /locus_tag="PLU99_14955"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOO18391.1"
                     /translation="MANKRDYYEVLGVARTAAPEEIKRAYRQAALKYHPDRNKSPDAE
                     GLFKEAAEAYEVLSDPEKRARYDRYGHAGLQGASMHDFSGMGIDDIFSVFSDLFGEAF
                     GGGRARTRGGRGVDIQALVEIDLRDVATGVEKTLRFERADYCDRCGGNGAEPGSKRRT
                     CRTCGGYGQVERQTSMGFFVTRTVTECPTCQGRGDTVEKPCRDCEGTGRVMKERVLNV
                     KIPPGIHDGQTIRIRGEGEPGPSGGSRGDLRCVVQIRRSQFFQRDGDHLVCVLPISFT
                     QAALGGQVDVPTLTGTAPLRVPPGTQHGTVFRLPGKGLPNLQTGRLGDEMVQVTIEIP
                     RKLNREQEELLRKFAATEDKQVLPESKGFFDRVKEYFTGEGATDQEERSSGSGAA"
     gene            complement(1434..3050)
                     /gene="groL"
                     /locus_tag="PLU99_14960"
     CDS             complement(1434..3050)
                     /gene="groL"
                     /locus_tag="PLU99_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564445.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOO18392.1"
                     /translation="MPAKQLMYSEHARRQMLNGLRKLSRAVASTLGPVGRNVLLQKSW
                     GSPRITKDGVTVSKEIELPDPFENMGAKLVNEVASKTSDVAGDGTTTATVLAEAIFSA
                     GLKNITAGANPTAVKRGIDAAVEVAVKSIKDQSVKVRGKDDIAKVATISANGDQEVGK
                     MLSEAFEKVGKDGVIEIEEGKSLETTWEHVEGMQFDKGYVSPYFITNPTTLETVLEDP
                     YILIYEKKISSLRDLIPVLEKIANVGKPLLIISEDLEGEALAALVVNKLRGVLHTCAV
                     KAPGFGDRRKAMLGDIAVLTGGIFISEDQGIKLENVDLNMMGRAKKVVVTKDETTIIE
                     GAGKKKDIEARIAQIRSQIEKTTSDYDREKLQERLAKMTGGVAVVKVGGATEINVKER
                     KDLVDDAFHATKAAAEEGIVAGGGVALLHAIKPVQAAAKKVEGDEAIGYRIIARALEE
                     PARQIAENAGVDGGVVVDEILSRGNNIGYDAARGEYVDMFEAGIIDPAKVARVALQNA
                     ASVAGLMLTTDVLCTEYKEEKHENIEGAMR"
BASE COUNT          466 a         1017 c          991 g          653 t
ORIGIN      
        1 ggtcaggcgc cccgcgccgc ctgtctgcga ttctaggcgg cgccgcttcc tgaggagcgc
       61 tcttcctggt cagtcgcgcc ctcgccggtg aaatactcct ttacgcgatc gaaaaagcct
      121 ttggattcgg gcagaacctg cttgtcctcg gtggcggcaa acttccgcag cagttcttcc
      181 tgctcgcggt taagcttacg cgggatttcg atcgtgacct ggaccatttc atcgcccagg
      241 cgcccggtct gcaggttcgg caggcccttg cccggcagcc ggaagaccgt cccgtgctga
      301 gtgccgggcg gcacgcgaag cggggcggtg ccggttaacg tcggaacgtc cacctgacca
      361 cccaaagcgg cctgggtgaa gctgatcggc agcacacaca ccaggtgatc gccgtcgcgc
      421 tggaagaact gcgaacgccg gatctgaacg acgcagcgca agtcgccgcg cgagccgccg
      481 ctggggccgg gctcgccctc gccgcggatg cggatggtct gcccgtcgtg aattcccggc
      541 gggatcttca cgttcagcac gcgttctttc atcacgcggc cggtgccttc gcaatcgcgg
      601 cagggctttt cgacggtgtc accgcggccc tggcacgtgg ggcactcagt gacggtgcgc
      661 gtcacgaaga accccatgga agtttgccgt tcgacctggc cgtagccccc gcacgtgcgg
      721 cacgtgcggc gcttggagcc gggctcggcg ccgttgccgc cgcaacggtc gcagtaatcg
      781 gcgcgctcaa aacgcagcgt tttctctacg ccggtggcga cgtcgcgcag gtcgatctcg
      841 accagggcct ggatgtccac gccgcgcccc ccgcgtgtgc gcgcccgccc gccgccgaaa
      901 gcctccccga acagatcgct gaagacgctg aagatgtcgt cgatgcccat cccggagaag
      961 tcgtgcatgc tggcgccttg caagcccgca tgaccgtaac gatcgtagcg ggcccgcttc
     1021 tccggatcgg acaggacctc gtaggcttca gcggcttcct tgaaaagtcc ctcggcgtcg
     1081 ggcgacttgt tgcgatccgg gtgatatttc aaggctgctt ggcggtaggc gcgcttgatt
     1141 tcctcgggcg cggcggtccg cgccaccccc agcacttcgt agtagtcgcg tttgttagcc
     1201 acggatccgt tcatagtcgc ccgccgcttt tggggcaaat cccgtgccgc ttttttttga
     1261 tcggcaggac cgcccgtggg ctgaccggtg aaaagccggc ctacccgcta agcggctgag
     1321 aaccggtccc tcaagcaaga caggggcggc tcgcgcgggg cgaccgtccc tgtcctggta
     1381 gaggacttcg ggcggggcaa agccgagctg tgccctacct cgacgaaggc ggactagcgc
     1441 atcgcgccct caatgttctc gtgcttctct tccttgtatt cggtgcacag tacgtcggtg
     1501 gtcagcatca ggccggcgac gctggccgcg ttttgcaaag cgacgcgggc caccttcgcg
     1561 ggatcgataa tgccggcctc gaacatgtcg acgtattcgc cgcgggccgc gtcgtaaccg
     1621 atgttgttgc cgcgcgacag gatttcgtcc actaccacgc cgccgtcgac gccggcgttc
     1681 tcagcgatct gccgggccgg ttcttccaag gcccgcgcga tgatgcggta gccgatggcc
     1741 tcatcgcctt cgaccttctt ggccgcagcc tgtaccggct tgatggcgtg caacagggcc
     1801 acgccgccac cggccacgat gccttcctcg gccgccgcct tagtggcgtg aaaagcgtcg
     1861 tcgaccaggt ccttgcgttc cttgacgttg atctcagttg cgccgccgac cttcacgacc
     1921 gccacgccgc cggtcatctt ggccagccgc tcctggagct tctcgcggtc atagtcgctg
     1981 gtggtcttct cgatttgcga gcggatctgg gcgatgcgcg cctcaatgtc cttcttcttg
     2041 ccggcgcctt cgatgatcgt cgtctcgtcc ttggtgacca cgaccttctt ggcgcggccc
     2101 atcatgttca ggtcgacgtt ctcgagcttg atgccctggt cttcgctgat gaagattccg
     2161 ccggtcagga ccgcgatgtc gcccagcatg gctttgcggc gatcgccgaa gccgggagcc
     2221 ttgacggcac aggtgtgcag cacgccgcgg agcttgttga ccaccagggc cgccagggct
     2281 tcgccctcga ggtcctcgga gatgatgagc agcggcttgc cgacattcgc gatcttctcc
     2341 agcaccggaa tcaggtcgcg cagcgaggag attttcttct cataaatgag aatgtacggg
     2401 tcttcgagca ccgtctcgag agtcgtcggg ttcgtgatga agtagggcga gacgtagccc
     2461 ttgtcgaact gcatgccttc gacgtgctcc caggtggtct cgaggctctt gccttcctcg
     2521 atctcgatga cgccgtcctt gccgaccttt tcgaaggcct cggacaacat cttgccgacc
     2581 tcttggtcgc cgttggcgct gatggtggcc accttggcga tgtcgtcctt gccgcgaact
     2641 ttgacggact ggtccttgat gctcttgacc gcgacttcga cggccgcgtc gatgccgcgc
     2701 ttcaccgccg tcgggttggc gccggcggtg atgttcttca gcccggcgct gaagatggct
     2761 tcggccagga cggtggccgt agtcgtgccg tcgccggcca cgtcggacgt cttgctggcc
     2821 acttcattga ccagcttggc gcccatgttt tcgaacgggt cgggcagctc gatttccttg
     2881 ctgacggtga cgccgtcctt ggtgatgcgc ggcgagcccc aggatttctg gagcagcacg
     2941 ttacggccga ccgggccgag cgtggaggcg accgcgcgag aaagcttgcg caggccgttg
     3001 agcatctgcc gccgggcgtg ttcagagtac attagttgct tagctggcat ttgtgactgt
     3061 cctttgttaa ctttcgctgg gtacagaccg ctcgtcagga gtcaaggtca gaatagccgt
     3121 aatcctg
//
LOCUS       DAOSYS010000342         3124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_24511_length_3124_cov_3.269469, whole genome shotgun sequence.
ACCESSION   DAOSYS010000342 DAOSYS010000000
VERSION     DAOSYS010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24511_length_3124_cov_3.269469"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLU99_14965"
     CDS             complement(<1..266)
                     /locus_tag="PLU99_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18393.1"
                     /translation="MRVWIVTSELPHEHAGGIGRYVDNFARTLGAAGHETLVLYRSAT
                     DTDDRPAPGVRTVGFAARDARLAEGGAARPDEHPAFPYNLLSFGP"
     gene            complement(270..617)
                     /locus_tag="PLU99_14970"
     CDS             complement(270..617)
                     /locus_tag="PLU99_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18394.1"
                     /translation="MEPVFDAAGFSFGSAHNSAASSVEYQRSRLQMLSTFLAQLTVAY
                     HEQAQWLWRESTRVGKQWQALEARIRELEVDREKAWAEAQRLATVWERQNRYIHQQNQ
                     RIAELEAQLRSGR"
     gene            complement(624..1949)
                     /locus_tag="PLU99_14975"
     CDS             complement(624..1949)
                     /locus_tag="PLU99_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOO18395.1"
                     /translation="MGERVVLLGIDGLDWSRLRAGVSAGRLPVFAELLETGAYAEVAV
                     TPCVPVLANLRNGLNSPALWTTVATGQYYFQHGVFEFLNLFESPTQPPLFASRHVRTP
                     RMWDVLSQYQHTSLVFGYYVTHPATALQGLMVSDLFGDVDDPSAVWPAERLDELARAL
                     GAPDYAAYRRGQGRLGTDVGIEQVCQAAPEQAELRKAAERILKEFTSLSPQSITRLLA
                     GEGDANERRLLEYRLIYPVIRDERFHRLLLRELPRGDWTLATIYYRTIDFVSHGFWTE
                     GHSLAPSFQHAYGEVIDRAYAHADAWVGEIRAQLGPGDRLIVLSDHGFRTNPTAADAT
                     QVKHIADLCRGDHAEPAVLIVSGGARRGRVDGVSLLDIAPSIYDLVGIPPTASLDGGA
                     VPGLLHAGAPRGLPPVSSYAPPPGCQREEIKSEEQDAIRRRLADLGYLE"
     gene            complement(1942..>3124)
                     /locus_tag="PLU99_14980"
     CDS             complement(1942..>3124)
                     /locus_tag="PLU99_14980"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOO18396.1"
                     /translation="NGSGKSTLLQIIAGTLRPDSGEVSVRGRVHALLELGSGFNPEFT
                     GRENVYLNGAVLGLPRREIEARFDQIADFADIGDFLDQPIKTYSTGMVVRLAFAVQAL
                     LAPDVLIVDEALSVGDEGFARKCYAWLENFRRAKGTLLFVTHNSQTVVQLCDRALLLD
                     HGELVAHGASKAVVDVYQKLLYCSPQQFAETRRRLRAVAGCAEQALAEESDVSPGDNG
                     EPEAPRVAYADPEVKSVSEVVYGTGEAEIFDAHTCDENGVPASVLVAGRPFEVRYRVR
                     FFQEARGVRFGLMLKTKEGIHLCGVSNVHLGWRTEHVAAGQVAVLRFRLRLNVSPGTY
                     YLNNGVAALGADGERFLHRRVDALAVRVIPPDKREIDGLVFTDPQVHCELMPDEETRR
                     G"
BASE COUNT          574 a         1074 c          941 g          535 t
ORIGIN      
        1 ggaccgaagc tcaacaggtt atatggaaag gcgggatgct catcgggtcg ggccgcgccg
       61 ccttctgcca gtcgagcgtc acgggctgcg aagcccacgg tccgcacgcc cggcgcgggt
      121 cggtcgtcag tatcggtcgc gctgcggtac aggacgagcg tttcgtgccc ggccgcgccc
      181 agcgtgcggg cgaagttgtc cacgtagcgc ccgattccgc cggcatgttc atgcggcaac
      241 tcggacgtga cgatccaaac gcgcattcct cacctcccgc ttcgtaactg cgcttccagt
      301 tccgcaatgc gttggttttg ctgatgaatg tagcggttct ggcgttccca cacggtggcc
      361 aggcgttgcg cctccgccca ggctttctcg cggtccactt ccagctcccg aatgcgggct
      421 tccagcgcct gccactgctt gcccactcgt gtgctttcgc gccaaagcca ttgcgcctgc
      481 tcgtggtatg cgaccgtcag ttgcgccaag aaggtgctga gcatctgcaa acgcgagcgc
      541 tggtactcca cactgcttgc ggcgctgttg tgagccgagc cgaaagagaa gccggcggcg
      601 tcgaaaactg gctccatcgc ggtctactcc agatatccca gatccgccag ccggcggcgg
      661 atggcgtcct gttcctcgga cttgatttcc tcgcgctggc atcccggcgg cggcgcgtag
      721 gaactcaccg gcggcagccc gcgcggcgca ccagcgtgca gcagaccggg gaccgcacca
      781 ccgtcaagac tggccgtggg tgggattccg accaggtcgt aaatgctggg tgcgatgtcc
      841 agcagactga ccccatcaac gcggccgcgc cgcgcgccgc ccgaaacaat cagtacagcg
      901 ggttcggcgt gatcgccgcg gcacaggtcg gcaatgtgct tgacttgcgt cgcgtcggct
      961 gccgtgggat tagtccggaa gccgtgatcg gacagaacga tcagccgatc gccgggtcct
     1021 agttgcgcac ggatttcgcc cacccaggca tccgcgtggg cataggcccg gtcaatcacc
     1081 tcgccatagg cgtgctggaa acttggtgcg agcgaatggc cctccgtcca aaatccatgc
     1141 gaaacgaagt caatcgtacg atagtagatt gtcgccaggg tccagtcccc gcgcggcagc
     1201 tcgcgcagca gtagccggtg aaaacgctca tcccgaatga cggggtaaat caaacggtat
     1261 tcgagcaagc ggcgctcatt cgcatccccc tccccggcca gcagtcgcgt aatgctctgc
     1321 ggcgacaagg atgtgaattc tttcaggatg cgctcggctg ccttgcgcag ctcggcctgc
     1381 tccggggctg cttggcagac ctgctcgatc ccgacgtcgg tgccaagccg gccctgcccg
     1441 cgccggtatg cggcatagtc tggcgccccc agcgcccgcg ccaattcatc cagccgttcg
     1501 gccggccaga ccgcgctcgg gtcatccacg tcgccgaaga gatcgctcac catcagcccc
     1561 tgaagcgccg tcgccggatg agtgacgtaa tagccgaaca ccaggctggt gtgctggtac
     1621 tggctgagca cgtcccacat ccgcggcgtg cgcacatggc ggctggcaaa cagcggcggc
     1681 tgagtgggtg actcaaacag gttcaggaac tcaaacacgc catgctggaa gtagtactgc
     1741 cccgtcgcga cggtggtcca taaagcaggc gagttcagac cgttccgcag attcgccagt
     1801 accggtacgc agggcgtgac ggccacttcg gcgtacgccc cggtttcgag cagttcggcg
     1861 aacacgggca ggcgcccggc tgaaacgccg gcgcgcagcc gggaccaatc cagcccgtca
     1921 attcccagca gcacgacgcg ctcacccacg tcgggtctcc tcgtccggca tcagctcaca
     1981 gtgcacctgc gggtcagtga agaccaggcc gtcgatttcg cgcttgtcgg gcgggatgac
     2041 gcgcaccgcc agcgcatcga cgcgccggtg caggaaacgc tccccatccg cacccagggc
     2101 cgcgacgcca ttgttcaggt aataggtgcc cggagagacg ttcagccgca ggcgaaaacg
     2161 caacaccgcc acctgtccgg cggcaacgtg ctccgtgcgc cagcccaggt gaacgttcga
     2221 tactccacac aggtggatgc cctctttggt cttaagcatc agcccgaagc ggacgccgcg
     2281 cgcttcctgg aagaatcgca cgcgatatcg cacctcaaat ggccgtccgg cgacgagcac
     2341 actggccgga acaccgtttt catcacaagt gtgcgcatca aatatctccg cttcgccggt
     2401 gccatagacc acttccgaaa cgctcttgac ctccgggtca gcatacgcga cccgcggcgc
     2461 ttccggctca ccgttatcgc ccggcgaaac gtcgctctct tcggccagcg cctgctcggc
     2521 acaaccggcg accgcccgca ggcggcggcg cgtctccgcg aattgctgcg gcgagcagta
     2581 gagcagcttc tggtaaacgt ccacgaccgc cttgctcgcc ccgtgcgcta ccaactcgcc
     2641 gtgatcgagg agcagcgccc gatcgcacaa ttggaccacg gtttggctat tgtgcgtgac
     2701 aaagaggagc gttcccttag cccgccggaa gttctccagc caggcgtaac acttgcgtgc
     2761 aaatccctca tcacccaccg acaatgcttc gtccacgatg agcacgtcag gcgccaggag
     2821 cgcctgcacc gcgaacgcca gccgcaccac cataccggtc gagtacgtct tgatcggctg
     2881 gtccaggaaa tccccgatgt cagcaaaatc cgcgatctga tcgaaccggg cctcgatttc
     2941 gcgccgcggc aaccccaaca ccgcgccgtt aaggtacacg ttctcgcgcc cggtgaattc
     3001 cggattgaaa cccgaaccca actccagcag ggcgtgaacc cggccgcgca cactcacctc
     3061 gccgctgtcg ggccgcagcg tgccggcgat gatctgcagg agcgtgcttt tacccgaacc
     3121 gtta
//
LOCUS       DAOSYS010000343         3080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_24945_length_3080_cov_5.691240, whole genome shotgun sequence.
ACCESSION   DAOSYS010000343 DAOSYS010000000
VERSION     DAOSYS010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24945_length_3080_cov_5.691240"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..903)
                     /locus_tag="PLU99_14985"
     CDS             complement(4..903)
                     /locus_tag="PLU99_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOO18397.1"
                     /translation="MTTDLSVLKDTVTVADGGWSTQLWERGVPVSQLPEMANLTHPEV
                     VVRLGREYVEAGARFITTNTFAANRYAIARRGVNTTVAELNQAGARLARQAVEGSEAL
                     VAGSIGPSGKILVVHEATEAELTIVFDEHARALAEGGVDVIVLETFSELAEILVALRA
                     AQGTGLPVIASLSFDSGPQRTRTIMGAAAEDCAAALEEAGAAVVGCNCGGGAANVLPA
                     VVALRGATKLPLWVKPNAGLPDLEDGRPVYRQPPDEFAAVVPTLIEAGANIIGGCCGT
                     GPEHIRRVAAIVENRKRPRRRDE"
     gene            complement(900..1712)
                     /locus_tag="PLU99_14990"
     CDS             complement(900..1712)
                     /locus_tag="PLU99_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOO18398.1"
                     /translation="MPQELPPAEQIAAVARAGFGGIELVLREDGPLSFDTALAEFQSL
                     ADHAASVGVSITSLATDFFWRVNYGAAGQADRARAMDMTVRMLDRAAAARAGAILVVP
                     AVVGRADEPRRRVAYADALNRAFEALLTLRFEAEDRGVVIAIENVWNRFLVSPLEMVE
                     LIDRVNSPCVAAYLDVGNVLAYGYPEDWIELLGRRLARVHVKDYDLRRPGAAGFCPLG
                     EGSVEWPAVIAALRKVAYAGPLTYEGPGELADIAARLRRILADNCDLAEEKT"
     gene            complement(1763..3055)
                     /locus_tag="PLU99_14995"
     CDS             complement(1763..3055)
                     /locus_tag="PLU99_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOO18399.1"
                     /translation="MAWKLRILTIGAVGAAFLQFACRHDDRDRQNCPPPDAGRPVIKT
                     QEELATERAAQIDAGEVAPTTSPAPLLAEAKPAPTRRAPAPLIRPAPGAIEAEVLLVN
                     DDVITVAEVLYPLRERIAELRAVQTPEGFVDSLNSLLRIRTQQAVGALLIHKKALAGL
                     AEHQKRIVEEGVERRYRETIDHEFGGSVARFERRLADGGLTAQQYRTLLERDLVAHEY
                     VREQLMPMVSVSRNEILNYYRLNRARYATEESRELAMIEAPFDAFLPPDASWDRALET
                     VRAQARLKAARHIRSAAEALAERPFEDVAREYSRGVHATHGGVWGEITRPLQPPYDGL
                     SKLIFDFEEGQVSEPIETQQGWYVVRCNKITPARNPAFTDVQDEIREILTEEKFQRLA
                     MDYMNKLASQATMSSLEVFLNAAARRAIHNEAGSRPEN"
BASE COUNT          525 a         1139 c          863 g          553 t
ORIGIN      
        1 gcgctactca tcccgccggc gcgggcgttt gcgattctcc acgatcgccg ccactcgccg
       61 gatgtgctcc ggccctgtcc cgcagcagcc cccgataatg ttcgcccccg cctcaatcag
      121 cgtcggtact acggccgcaa actcgtccgg cggctgccgg tagaccgggc gcccatcctc
      181 caaatccggc agacccgcgt tcggcttgac ccatagcggc agcttggtcg ccccgcgcag
      241 ggccacgaca gccggcaaga cattcgccgc gcccccgccg cagttgcacc cgaccaccgc
      301 ggcgccggct tcttccaggg cggccgcaca gtcctccgcc gccgccccca tgatcgtccg
      361 cgtacgttgt gggccggaat caaaggacag gctggcaatt accggcaggc cggtgccctg
      421 tgccgcccgc aatgcgacca gaatctccgc cagctccgag aacgtctcga gcactatcac
      481 gtccacgccg ccttcggcca gggctcgcgc gtgctcatca aagacaatcg tcagctcggc
      541 ttcagtcgcc tcgtgcacca ccagaatttt cccgctcgga ccaattgagc cggctacgag
      601 cgcttcgctg ccctcaactg cctggcgggc cagccgggcg ccggcctggt tcagctccgc
      661 caccgtcgtg ttaactccac gccgggcaat ggcgtaccgg ttcgcagcaa aagtgttcgt
      721 ggtgatgaat cgcgccccgg cctccacgta ctctcgccct agcctgacta ccacttctgg
      781 atgtgtcagg ttggccattt ccggcaattg gctcaccggt accccgcgtt cccaaagctg
      841 agtggaccac ccgccatccg cgacagtcac tgtatctttg agcaccgaca ggtccgtcgt
      901 cacgtcttct cctcggccaa atcgcagttg tctgccagaa tccgtctcaa ccgggccgca
      961 atgtctgcca actcgccggg accttcgtac gttagcggcc cggcataggc gaccttgcgc
     1021 agcgccgcaa ttaccgccgg ccactcgaca ctgccttcgc caagcgggca aaaacccgcc
     1081 gcaccgggcc gtcgcaaatc atagtctttc acgtgtacgc gcgccagtcg ccttcccagc
     1141 agctcgatcc agtcctccgg atacccgtac gccagtacgt tgccgacgtc cagataggcc
     1201 gcaacacatg gcgagttgac gcgatcaatc agctcgacca tctccagcgg agataccaaa
     1261 aagcgattcc agacattctc aatcgcgatc accacgcccc gatcttcggc ctcaaaccgc
     1321 agcgtcagca atgcttcgaa tgcccggttc agcgcgtccg cgtacgccac ccggcgccgc
     1381 ggctcatcag cccggccgac taccgccggc acgaccagta tcgctcccgc ccgcgccgcg
     1441 gccgcacgat ccagcatccg cacggtcata tccatggctc gcgcccgatc ggcctgcccc
     1501 gcggccccgt aattgacccg ccagaagaaa tcagtagcca ggctggtgat gctcacgccg
     1561 accgaagcgg cgtgatcggc cagactttga aactcggcca gagccgtatc aaacgaaagc
     1621 ggcccatctt cgcggagcac caactcgatt ccgccaaacc ccgcgcgggc caccgccgct
     1681 atctgctccg ccggcggcag ttcttgcggc aagctccagg cattaacggc agcaaacaaa
     1741 ctggtccccg gttccccgct tttcagtttt ctggtctcga accagcctcg ttatgaatcg
     1801 cgcggcgcgc tgcggcgttc agaaagacct ccaaggacga catcgtcgcc tgcgacgcca
     1861 gcttgttcat gtaatccatc gccagcctct gaaacttctc ctccgtcaaa atctcgcgga
     1921 tctcgtcttg aacgtccgtg aacgccgggt ttcgcgccgg cgtaatcttg ttgcagcgca
     1981 ccacgtacca gccctgctgc gtctcgatcg gctcgctgac ctgcccctcc tcaaaatcga
     2041 atatgagttt actcagcccg tcgtacggcg gctgcaacgg ccgcgtgatc tcgccccaca
     2101 ccccgccgtg ggtcgcatgc accccgcgcg agtattcgcg cgccacgtcc tcgaacggcc
     2161 gctccgccag cgcctcggcc gcgctgcgaa tgtgccgcgc ggctttcaag cgggcctgcg
     2221 cccggaccgt ctcgagggcc cggtcccaac tcgcgtccgg cggcaggaat gcgtcgaagg
     2281 gggcctcgat catcgccagt tcgcgactct cttcagtggc gtagcgggcg cgattgaggc
     2341 ggtagtagtt cagaatctcg tttcgcgaga cgcttaccat cggcattaac tgctcgcgca
     2401 cgtattcgtg cgccaccaga tcccgttcca ggagcgtgcg gtactgttgg gcggtcagac
     2461 cgccgtccgc gaggcgccgt tcgaaacgtg ccacgctacc cccgaattcg tggtctatcg
     2521 tctcgcgata gcggcgctct acgccctcct ccacaattct cttctggtgc tccgccaaac
     2581 cggccagggc ctttttgtgg atgagcagcg ctccgaccgc ctgctgcgtc cgtatgcgca
     2641 gcaggctgtt gaggctgtcc acgaaaccct cgggcgtctg caccgcgcgc agctcggcga
     2701 tccgctcccg gagcgggtac agcacctccg ccacggtaat cacgtcatca ttgaccagta
     2761 gcacctcggc ctcgatagcg cccggcgccg gccggatcag cggggccggc gcccgccgcg
     2821 tcggggcagg cttggcctca gccagcagtg gagcgggaga cgtggtcggc gccacttccc
     2881 ccgcgtctat ttgcgcggcc cgttcggtgg ccagttcctc ctgcgtcttg ataaccggcc
     2941 gcccggcatc cggtggcgga caattctggc gatcgcggtc gtcatggcgg caagcgaatt
     3001 ggagaaaggc tgcacctacc gcgccgattg tcaaaatgcg caatttccag gccatgagcc
     3061 gtaatatagc ggctcaggcc
//
LOCUS       DAOSYS010000344         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25008_length_3073_cov_4.415838, whole genome shotgun sequence.
ACCESSION   DAOSYS010000344 DAOSYS010000000
VERSION     DAOSYS010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25008_length_3073_cov_4.415838"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU99_15000"
     CDS             <1..886
                     /locus_tag="PLU99_15000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOO18400.1"
                     /translation="RWTVNPYRGCQHACAYCYARRTHEYLGLGAGTDFESRIIVKINA
                     PELLADELARPAWRHENIAFSGVTDAYQPLEAVYRITRRCLEACLAAANPVGIVTKSY
                     LIVRDTDLLVELHRRAHVSVSFSIPFADAQVARRLEPHAPPPARQFEAMRRLATAGVP
                     VGILLAPIIPGLNDRDIPQLLKQAVECGAGQAGYAPVRLPGSVQQVFLSRLRAELPDA
                     AARIEQRTRELRGGQLNDPRFGYRMQAAGAYWESVTRLFETTATRLGLDCGQPWRQNQ
                     CPGPGERSRPRQLSLFKT"
     gene            1019..2248
                     /gene="obgE"
                     /locus_tag="PLU99_15005"
     CDS             1019..2248
                     /gene="obgE"
                     /locus_tag="PLU99_15005"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOO18401.1"
                     /translation="MARASGTERLFVDEVTIEVRGGKGGDGVVSFRREKFVPKGGPDG
                     GDGGRGGSVYLEARAGLSTLLDLAGRHHYRAQDGQPGSGANRHGKDGKDLIVPVPAGT
                     LVYNAETGRLLKDLVQDGQRVRVAAGGRGGYGNQHFATSTNQAPRTAERGRPGQERKL
                     RLELKLIADIGLVGLPNAGKSTLLARLTNARPKIAAYPFTTLDPQLGILELPGYRRLV
                     LADIPGLIEGAHEGVGLGDAFLRHIERTRVIVHLVDLCPPEGMPRPFEAYQILRRELE
                     KYSPALAQREEIIVGNKLDLTEAAESLAQLRGQLSGEVLAISGVTGQGVRELAEAMWE
                     RVQRVRETEAAAQPTQIDFGEPAAEEPAEAVDEPAEADNGGADDSAVEDIDDRSWGGN
                     VMNNQDDSPQSGSFDED"
     gene            2238..3026
                     /locus_tag="PLU99_15010"
     CDS             2238..3026
                     /locus_tag="PLU99_15010"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOO18402.1"
                     /translation="MKTSYLKPVDALMVVDIGNTRIGLGTWDSDGIHDVIRVPIAQPG
                     VWAEAVRKAWSGSEDAGRRAVALSSVAPDQTQRLTAVIEEVCRTEALSIPEDLPLPLK
                     IDVDEPEELGVDRVCSAAAAFERVGGPCAIASFGTATTTDCVSQDGVFMGGTLAPGLQ
                     LSCDALHAHTALLPRVEVAEPGGPFGRTTKEAILSGVALGAVGALREIVEQFATALGR
                     WPYLVITGGNAALIGKLADFVDAVVPDLSLMGVALAYRKAAGQA"
BASE COUNT          542 a          964 c         1047 g          520 t
ORIGIN      
        1 tcgctggacg gtcaatccgt accgcggctg ccagcacgcc tgcgcttact gctacgcccg
       61 gcgcacgcac gagtatctcg gcctcggcgc cggcactgat ttcgaaagcc gcattatcgt
      121 caagatcaac gcccccgagc ttctggcaga tgaactcgca cggcccgcct ggcggcacga
      181 gaacatcgcc ttctccggcg ttacggacgc ctaccagccg ctggaagcag tctatcgcat
      241 cacgcgccgc tgcctcgaag cgtgcctcgc ggccgccaac cccgtgggca ttgtgaccaa
      301 atcgtatctc atcgtgcgcg acacggactt gctcgtcgaa ctgcatcgcc gcgcgcacgt
      361 cagcgtcagc ttcagcatcc ccttcgcgga cgcgcaggtc gcacgccggc tcgaaccgca
      421 cgccccgccg ccggcccggc agttcgaagc catgcgccgc ctggctacgg ccggcgtgcc
      481 ggtgggcatc ctgctggccc ccatcattcc gggtttgaac gaccgcgaca tacctcagct
      541 tctgaagcag gcggtggaat gcggcgcggg ccaggccggt tacgccccag tgcgcctgcc
      601 cggcagcgtg cagcaggtct tcctgtcgcg attgcgggcc gaactgcccg acgccgcggc
      661 gcgcatcgag cagcgcacgc gcgagctgcg cggcgggcag ttgaacgacc cgcgtttcgg
      721 ctatcgcatg caggccgccg gcgcctactg ggaaagcgtc acgcgactct tcgaaaccac
      781 cgccacgcgc ctcggcctgg attgcggcca gccgtggcgt caaaaccagt gccccggccc
      841 gggcgaaagg tcacgtccac gccagctttc gctgttcaaa acttgagatt cggcgagcta
      901 gggcagtcgg cggggctcat gacttctcct cgacccttga tttccggctc ccttgactcc
      961 tattttcctt acgccgaatt gattgacaca tcattaaacc tgtgttgcac tcgctcctat
     1021 ggcacgggca agcggaaccg aacggctatt cgttgatgaa gtcaccatcg aggtgcgcgg
     1081 cggcaaaggc ggcgacggcg tcgtcagctt ccgccgcgag aagttcgtgc ccaagggcgg
     1141 gccggacggc ggcgacggcg gccgcggcgg gtcggtgtac cttgaagctc gggcggggct
     1201 ctcgacattg cttgacttgg ccgggcgaca tcattaccgc gcgcaggatg gccagccggg
     1261 ctccggtgcc aatcgccatg ggaaagacgg caaggacctg atcgtacctg ttccggccgg
     1321 gacgctggtc tacaacgccg agaccggccg gctgctcaaa gaccttgttc aagatggcca
     1381 acgagtgcgt gtcgcggccg gtggacgcgg cggctacggc aaccagcatt tcgccacgtc
     1441 taccaaccag gcgccccgca ccgctgaacg cggccggccg gggcaggagc gcaagctgcg
     1501 gctggagctg aagctgatcg ccgacatcgg gctggtgggg ctgcccaacg cgggcaagag
     1561 cacgctgctg gcacggctga cgaacgcgcg gccgaagatt gccgcttacc cgtttacgac
     1621 actcgacccg cagcttggga ttttggagct gccgggctat cggcgcctcg tgctggcgga
     1681 catacctggg ctgatcgaag gggcgcacga gggagtggga ctgggtgacg ccttcctgcg
     1741 gcacatcgag cggacgcggg tcatcgtgca cctggtcgat ttgtgtccgc ccgagggaat
     1801 gccaaggccg tttgaggcct accaaatact ccgccgagaa ctggagaaat acagcccggc
     1861 gctggcccag cgcgaggaaa ttatcgttgg caacaagctg gacctcacgg aagctgccga
     1921 aagtctggca cagttgcggg ggcaactaag tggcgaggtt ttggccatct ccggcgtaac
     1981 cggccaaggc gtacgcgaac tggctgaggc catgtgggag cgcgtgcaac gagttcggga
     2041 gacggaagct gcggcgcagc ccactcaaat tgattttggc gagccggcgg ctgaggaacc
     2101 tgccgaagcg gttgatgaac ctgccgaagc ggataatggc ggtgcggacg attcggcagt
     2161 tgaggacatt gacgaccgct cttggggcgg taacgtcatg aataaccaag acgattcgcc
     2221 gcagtcggga tcatttgatg aagactagct atctgaaacc ggtcgacgcg ctgatggtgg
     2281 ttgacatcgg caatacgcgc atcggcctgg gcacgtggga ttctgacggc atccatgacg
     2341 tgatccgcgt gccaatcgcc cagccgggag tctgggcgga agccgtcagg aaagcctgga
     2401 gcggcagcga ggacgccggg cgccgcgccg tggctctgtc gagcgtcgcg ccggaccaga
     2461 cccagcgttt gacggctgtt atcgaagagg tctgccgaac ggaagcgctc tcgattcccg
     2521 aggacctccc tttgccgttg aagatcgacg tggacgaacc ggaggagttg ggcgtggacc
     2581 gggtgtgctc ggcggcggcc gccttcgagc gcgtcggcgg gccatgtgcg atcgcttcgt
     2641 tcggaacggc gacgacgacc gactgcgtgt cgcaggacgg cgttttcatg ggcggcactc
     2701 ttgcgccggg cctgcagttg tcgtgcgacg cgctgcacgc ccacaccgcg ttgctgccgc
     2761 gcgtggaggt ggccgagccg ggcggcccgt tcgggcggac cacaaaggaa gccatactga
     2821 gcggcgtggc tttgggagcg gtaggggccc tgcgcgaaat cgtggagcaa ttcgcgacgg
     2881 cgctggggcg ctggccatac ctggtgatca ccggcgggaa cgcggcgttg atcggcaagt
     2941 tggccgattt cgttgacgcc gtggtgcctg atctttcgct gatgggggtg gcgctggcgt
     3001 atcgcaaagc ggccggtcag gcatgaatac ggccgaagat ggggcacttg ggataaacag
     3061 accgcttgaa aac
//
LOCUS       DAOSYS010000345         3072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25023_length_3072_cov_4.656613, whole genome shotgun sequence.
ACCESSION   DAOSYS010000345 DAOSYS010000000
VERSION     DAOSYS010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25023_length_3072_cov_4.656613"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PLU99_15015"
     CDS             complement(<1..1083)
                     /locus_tag="PLU99_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOO18403.1"
                     /translation="MSEPPQPPVSATYRIQLTPDFGFDQAAAQVDYWAALGVTHLYCS
                     PYLEAHPGSQHGYDIISHTRANPELGGEEGRARLAQALAARGIGQVLDIVPNHMAATS
                     RNSIWQEVLRNGPNSRYANFFDIDWQTRQPRLRGRVILPILGDHYGRTLRAGQLKLRR
                     VSGSFGFQYFEETLPLAPETLTEVLSPAANKCGSDELAFLADALARLPQDATAESGAT
                     RSRDEQVLYTQLARLLSEQPAIAAAVDEVIAEINGEIDKLDGLLSRQHYRPAYWRSSL
                     SELNYRRFFNITTLVALRVEEPEVFAESHARVLEWVRAGAVQGLRVDHIDGLRDPLQY
                     LRRLRSAVPQAWIVLEKILQRGERLPE"
     gene            complement(1076..>3072)
                     /gene="glgX"
                     /locus_tag="PLU99_15020"
     CDS             complement(1076..>3072)
                     /gene="glgX"
                     /locus_tag="PLU99_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOO18404.1"
                     /translation="VQVCFFDDEDNETAVDLPEQTAFCWHGYFPTIGPGQRYGYRVHG
                     RWNPREGQRCCAAKLLLDPYAKAIAGEPRWNPALYAYRPDDPNGPPNDTDSAPYMPKC
                     VVVNPYYDWSRDHRPGYSLNESIIYELHVKGFTASFPDIPPELRGTYAGLAHPAVLKY
                     LTSLGVTAVELMPVHQFVHEQHLQERGLRNYWGYNSICYFAPHNEYASVNKIGQQVQG
                     FKAMVQHLHDAGLEVILDVVYNHTAEGNHLGPTLCYKGIDNAAYYRLHPDKKEYYMDY
                     TGTGNSLNMRHPHVLQLIMDSLRYWVVDMHVDGFRFDLAATLARELHDVDRLSAFFDI
                     IHQDPVLSQVKLIAEPWDVGEGGYQVGKFPPQWSEWNGKYRDAVRDYWRGREGSLGEI
                     ATRFSGSADLYEGTGRRPHASVNFVTCHDGFTLNDLVTYDHKHNGLNGEDNADGNNDN
                     RSWNCGVEGPADDPKVNSLRARQKRNFMATLLLSQGIPMLSHGDEIGRTQNGNNNAYC
                     QDNETTWVDWQNRDEQLLAFVMEMIRLRKEHPTFRRWKWFQGQTSLSRGVQDIAWYGP
                     DGAEMTEDDWNGKALQVFLSGSLDAMDARGQPIVDRDFYVLFNAQEVTVSFTLPQRGD
                     GRKWQRIVDTAVANLDEKLVYSEGAQVAVEMHSLVVLCNV"
BASE COUNT          517 a          943 c          991 g          621 t
ORIGIN      
        1 ctcgggcagc cgctcgccgc gttgcaaaat cttttcgagc acgatccagg cctgcggtac
       61 ggcggaccgc aaacgccgca ggtactgcaa cgggtcgcgc aggccgtcaa tgtgatcgac
      121 gcgcaggcct tgaaccgcgc cggctcgcac ccattccagc acgcgggcgt ggctttcggc
      181 aaagacctcg ggctcctcca cgcgcagcgc caccagcgtg gtgatattga agaagcgacg
      241 gtagttcagc tcactgagcg agctgcgcca gtaagcaggg cggtagtgct gccggctgag
      301 caggccatcc aatttgtcga tttcgccgtt gatttcagcg atcacttcat caactgcggc
      361 cgcgatcgca ggctgctcac tcagaaggcg ggcaagctgc gtgtagagca cttgttcgtc
      421 acgactacgc gtcgcgccgg attcggccgt cgcgtcttgc ggcaggcgtg ccagcgcgtc
      481 ggccaggaaa gccagttcgt ccgagccgca cttgtttgcg gccggtgaaa gcacttcagt
      541 cagcgtttca ggagccagcg gtaacgtttc ttcgaaatac tggaagccga aggagccgga
      601 aacgcgtcgc aatttgagct gtccggcccg caatgtccgg ccgtaatgat cgccgagtat
      661 gggcagaatg acccggccgc gcaagcgcgg ctgccgcgtt tgccagtcta tatcgaagaa
      721 attcgcatag cggctgttcg gcccattcct gagcacctcc tgccaaatgg agttacggct
      781 ggtggcggcc atgtggttcg ggactatgtc gagcacctgt ccgatgccac gggctgcgag
      841 ggcctgtgcc agtcgcgcgc ggccttcttc gccgcccagt tccgggttcg cgcgagtgtg
      901 acttatgatg tcgtagccgt gctgactgcc cgggtgggcc tccaggtagg gcgagcagta
      961 gaggtgcgtg acaccgagcg cggcccagta gtccacctgg gccgcggcct ggtcaaagcc
     1021 gaaatcgggg gtgagttgaa tacggtaggt tgcgctgacg ggcggctgcg gcggctcaga
     1081 cattacacag caccaccagc gagtgcattt cgacagccac ctgcgccccc tcggagtaca
     1141 ccagcttttc gtccaggttt gcgaccgcgg tatccacgat ccgctgccat ttccggccgt
     1201 cgccgcgctg cggaagtgtg aaactgaccg tcacctcctg ggcgttgaac aggacgtaaa
     1261 aatcccggtc gacgatgggc tggccgcgtg cgtccatggc gtcgagactg cccgagagga
     1321 acacttgcag agccttgccg ttccagtcgt cctcggtcat ttccgcgccg tccggaccgt
     1381 accaggcgat atcctgtacg ccgcgtgaca agctggtctg cccctgaaac catttccaac
     1441 gccggaaggt ggggtgctcc ttccgcagtc ggatcatttc catcacgaac gccaggagtt
     1501 gctcgtcgcg gttctgccag tcgacccagg tagtttcatt atcctggcaa taggcgttgt
     1561 tgttgccgtt ttgcgtgcgg ccgatctcgt cgccgtgcga gagcatgggg atgccctgcg
     1621 agagcagcag ggtggccatg aagttgcgct tttggcgcgc ccgcagggag ttgactttgg
     1681 ggtcgtccgc cgggccttcc acgccgcaat tccaagaacg gttatcgttg ttgccgtcgg
     1741 cgttgtcctc gccgttgagt ccgttgtgct tgtggtcgta cgtcaccagg tcgttaagcg
     1801 tgaagccgtc atggcaggta acgaagttca cgctggcgtg cgggcggcgg ccggtgccct
     1861 cgtacaggtc ggcgctgccc gagaagcgcg tggcgatctc gcccaggctg ccctcgcgcc
     1921 cgcgccagta atcgcgcacc gcgtcccggt acttgccgtt ccactccgac cattgcggcg
     1981 gaaacttgcc gacctggtag ccgccttcgc ccacgtccca cggctcggcg atcagcttca
     2041 cctggctgag gaccgggtcc tggtggatga tgtcgaagaa ggccgacagg cgatcgacat
     2101 cgtgcagttc gcgggccagc gtggccgcca ggtcgaaacg gaaaccgtct acgtgcatgt
     2161 cgactaccca gtagcgcaac gagtccatga tgagctgcaa gacgtgcggg tggcgcatgt
     2221 tcaggctgtt gccggtaccg gtgtagtcca tgtagtactc tttcttgtcc gggtgcaacc
     2281 ggtagtaagc ggcgttgtct atccccttgt agcagagcgt agggcctaga tggttgccct
     2341 cggctgtgtg gttgtagact acgtccagaa tgacctccag cccggcgtcg tgcaggtgtt
     2401 gcaccatcgc tttgaagccc tgcacctgct ggccgatctt gttgacggat gcatattcat
     2461 tgtgcggcgc gaagtaacag atggagttgt agccccagta attgcgcagc ccgcgttcct
     2521 gtaagtgctg ttcgtgaacg aactggtgta ccggcatcag ctcgaccgcc gtcacgccca
     2581 ggctggtcag gtatttcagc accgccgggt gtgccaggcc ggcgtaggtt ccgcgcagct
     2641 cgggcggaat atccgggaag ctcgccgtaa aacccttgac gtgcagctcg taaattatgc
     2701 tctcgttcaa gctgtagccc gggcggtgat cgcgcgacca gtcgtagtaa gggttcacga
     2761 ctacgcactt cggcatgtac ggggcgctgt ctgtgtcgtt cggcgggccg ttcgggtcgt
     2821 cgggccggta ggcgtatagc gcagggttcc aacggggctc gcccgcgatg gccttggcgt
     2881 acgggtcgag cagcagcttg gccgcacagc agcgctggcc ctcgcgcggg ttccagcgcc
     2941 catgcacgcg gtatccgtaa cgctggcccg ggccgatcgt aggaaagtac ccgtgccagc
     3001 agaacgccgt ctgctccggc aggtccaccg ccgtctcgtt gtcctcgtcg tcgaagaagc
     3061 acacttgcac cc
//
LOCUS       DAOSYS010000346         3069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25058_length_3069_cov_4.907432, whole genome shotgun sequence.
ACCESSION   DAOSYS010000346 DAOSYS010000000
VERSION     DAOSYS010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25058_length_3069_cov_4.907432"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PLU99_15025"
     CDS             <1..1261
                     /locus_tag="PLU99_15025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO18405.1"
                     /translation="PIRTPGYPLFLAGIFQVFGPGPVSILIFQHILGVASCGLIAYAA
                     ARLTRSRVGSIAGLLLALDPWLFALESYALSETLTVALLAFAIALTLGPARPRAYHAL
                     LIGMALGAACLTRPACQVLVPFVGLAWLVRAGAGRNWRGLLIGATAGIIGIGGVLGPW
                     VLFNKRRNVDGVASGYSEMQFKGLLRFGLLERDFPGDPELAAAFAELGEANPTWAQMR
                     TFVNTHIRPWSARTGRLSEWNRASIAANFGEYVRHCGYALAWQLNWFPEHGPKPGYSS
                     WPDWSLQRLARDGSNKQYEGDSKRLGLDAFAMERKCGKLGALLGWLGEHASGGRPQMV
                     VFGMALAATVLALVGRRWSVALLVIGSFAFTLAHVMMLFPVGRYLVPMMPVWYLCVGL
                     VLAAVVGWPKRRMTAATAATRLRRGAV"
     gene            1324..2412
                     /locus_tag="PLU99_15030"
     CDS             1324..2412
                     /locus_tag="PLU99_15030"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO18406.1"
                     /translation="MKLVVIIPALNEAATIAGVIQRIPARIPGVTRCETIVVDDGSSD
                     ATAEEARAAGARVVQHPVNRGVGAAFKTGVEAALEAGADIVVNMDADGQFRPEDIPRL
                     IAPILAGKAGFVTCTRFADPAHLPQMPWIKRWGNAGMTWLIGRLCGRKFTDVSCGFRA
                     YTRETLLRLNLYGRYTYTQEMFLNLAVQDVPMAEVSLSVRGTREFGKSRVAGSITKYI
                     RKTVPIIFRTLRDVRPFAFFGTIAAVLLLGGLGAEGFVFGHWCLTGRTHPYQALITVG
                     AVGIILGFLVLVIALVADMLNRLRRLLENVLYYVRSAHYARAVPFTHRLRDEGPEVIC
                     EARLPVEAIAPHESYDDEAALAPLEKSS"
     gene            2526..>3069
                     /locus_tag="PLU99_15035"
     CDS             2526..>3069
                     /locus_tag="PLU99_15035"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOO18407.1"
                     /translation="MLRAAVQQISRLVPGVRFTLLSLYPADDAAENRDPALKIAPLSP
                     LSMVAVAFPLALLAGLLRRLRLPYRFLLRTPALRALDAADLVIDLSGISFVDGRGTII
                     IYNVLVVLLPVLLGKPLLKYAQALGPFRGRLNRFCARWLLPKTARIVARGRITRAHLD
                     ELGLPPGLVVDSTDAAFAMEV"
BASE COUNT          459 a          971 c         1029 g          610 t
ORIGIN      
        1 gcctattcgc acgccgggat atccgctctt cctggccggc atctttcagg tgttcgggcc
       61 tgggccagtt tctatcctga tcttccagca tattctcggc gtcgcctcat gtgggcttat
      121 cgcgtatgcg gccgccaggc tcacgcgctc ccgggtgggc agtatcgccg gattgttgct
      181 ggcgctggat ccctggctgt ttgcacttga gagctatgcg ttgtcagaga cgctcaccgt
      241 ggctttgctg gcctttgcga ttgcgttgac gctcggcccg gcacgaccgc gcgcatacca
      301 cgccctgctg atcggaatgg ccttgggcgc ggcttgtctg acgcgtccgg cctgccaggt
      361 attggtgcca tttgtcggac tggcctggct ggtgcgagcg ggggcgggca ggaactggcg
      421 tggcctgctg attggcgcga ccgcggggat catcgggata ggaggggtac tcggtccctg
      481 ggtgctattc aacaagcggc gtaatgtcga tggagtggcc tcgggctaca gcgagatgca
      541 gttcaagggg ctgctgcggt tcggcctgct tgagcgcgat ttcccgggcg atcctgaact
      601 ggcagcagcc ttcgccgaat tgggcgaggc aaacccgacg tgggcccaga tgcgaacgtt
      661 cgtcaacact cacattcggc catggagtgc ccgcacgggg cgtttgtcgg aatggaaccg
      721 cgccagcatt gcggcgaatt tcggggagta cgtgcgacac tgcggctacg ctttggcgtg
      781 gcaactgaac tggtttccag agcacgggcc taaaccgggc tactcttcct ggccggactg
      841 gtcgctgcag cgcctggcgc gcgacggttc aaacaaacag tacgagggcg actcgaagcg
      901 cctagggctg gacgcattcg ccatggaacg caagtgcggc aagctcgggg cgctgctggg
      961 ctggctcggt gagcatgcgt ccggcgggcg gccacagatg gtggtgttcg gaatggctct
     1021 ggcggcgacg gtcctggcgc tggttgggcg gcgttggagc gtggcgctgt tggtgatcgg
     1081 atccttcgca ttcacacttg ctcacgtaat gatgcttttt ccggtcggac gctacctggt
     1141 cccgatgatg ccggtctggt acctgtgcgt ggggctggtg cttgcggcgg tggtgggttg
     1201 gccgaagcgc cgcatgaccg cggccacggc agcgacgagg ttgcgacggg gcgccgtcta
     1261 aattagtatg ctcgcaggag cgcggtcgca gaagcgcaag ttcgcctctt cggttcgagc
     1321 cgcatgaagc tggtcgtcat cattcccgca ctgaatgaag ccgcaacaat agccggcgtc
     1381 atccagcgca ttccggcgcg cattcccggc gtaacgcgct gcgaaactat cgtcgtcgat
     1441 gacggctcgt cggacgcaac cgccgaggag gcccgcgcag ccggcgcgcg ggttgtgcag
     1501 catccggtca accgcggcgt gggggcggcc ttcaaaacgg gcgtggaagc tgcgctcgag
     1561 gccggtgcgg acatcgtcgt caatatggat gccgacgggc agtttcgccc ggaagacatt
     1621 ccgcgcctga ttgctccgat tctcgccggg aaagccggct tcgtcacttg cacgcgcttc
     1681 gccgacccgg cgcacctgcc gcagatgccc tggatcaaac gctggggtaa tgccggcatg
     1741 acctggctca tcggccgcct gtgcggacgc aaattcaccg acgtctcctg cggcttccgg
     1801 gcctacacgc gcgaaacgct gctgcggctg aatctctacg gccggtatac ctacacacag
     1861 gaaatgttcc tgaacctcgc cgtccaggac gttcccatgg cagaggtctc cctgtcggtg
     1921 cggggcacgc gcgaattcgg gaaatcgcgc gtggcgggca gcatcacgaa gtacatccgc
     1981 aagaccgtgc cgatcatctt ccgcacgctg cgcgacgtcc gcccctttgc cttcttcggg
     2041 acaatcgcgg cggtgttgct gctgggcggc ctgggcgccg agggcttcgt cttcggccac
     2101 tggtgtctca cgggccgcac gcacccctat caggctctga tcaccgtcgg cgcggttggc
     2161 atcatcctcg gcttcctcgt gctcgttatt gcactagtcg cggatatgct gaaccgcctg
     2221 cgccgcttac tggagaacgt gctgtactac gtacgctcgg cacattacgc gcgcgccgtt
     2281 ccgtttaccc atcggctgcg cgatgaaggc cccgaggtta tctgcgaggc ccgcctgccg
     2341 gttgaggcaa tcgcgccgca cgagtcctac gacgatgaag cggcgctagc cccgttggag
     2401 aagtcctcgt gagcctcgga cccgccgctg ccgctgcaag cgcggctcct gatccggctc
     2461 tcccgcccgc aaagcgcgtc gcactgctag gcgcgaccct cactggaaac cgcggagcgg
     2521 aagccatgct gcgggcggcc gtgcagcaga tctcacgcct tgttccgggc gtacgcttca
     2581 cgctgctcag cctttatccg gcggacgatg ccgcagaaaa ccgcgaccca gcgctgaaga
     2641 tcgcgccgct gtcgcccctg agcatggtgg cggtggcctt tccgcttgcc ctgctcgcgg
     2701 gcttgctgcg ccggttgcgg ctgccgtacc gattcttgct gcgcaccccg gccctgcggg
     2761 ccctggacgc ggctgacctg gtgatcgatc tatcagggat cagtttcgtc gacggccgcg
     2821 gcacgatcat tatctacaac gtgctggtcg tgctgctgcc ggtgctcctc ggcaagccgc
     2881 tgctgaaata cgcgcaagcc ctgggtccgt ttcgtggccg tctgaaccgc ttttgtgcgc
     2941 gatggctgct gccgaagacg gcccgcatcg tggcgcgagg acggatcacg cgggcgcacc
     3001 tggacgaact tgggctgccg ccggggctcg tggtggattc caccgatgcc gcgttcgcca
     3061 tggaggtcg
//
LOCUS       DAOSYS010000347         3020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25564_length_3020_cov_4.990556, whole genome shotgun sequence.
ACCESSION   DAOSYS010000347 DAOSYS010000000
VERSION     DAOSYS010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25564_length_3020_cov_4.990556"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLU99_15040"
     CDS             <1..398
                     /locus_tag="PLU99_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOO18408.1"
                     /translation="TVPQAEALDAGLKASGRTLQAVIDFRLDDAEIIRRIITRRVCID
                     CGAAYNTEFMPPKKAGVCDRCNGEVIQRVDDREETVRTRLATYRAQTEPLIGYYTRQG
                     LLQSVDASVGADAVQKEVLRIVEALGTRG"
     gene            395..1195
                     /gene="map"
                     /locus_tag="PLU99_15045"
     CDS             395..1195
                     /gene="map"
                     /locus_tag="PLU99_15045"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOO18409.1"
                     /translation="MKAAGARIVLKSAREIELMRAAGRLVYEILCELQRRIRPGVTTA
                     ELDRVAGQMIRDAGATALFLGVRNPQARCPFPASICASVNEAVVHGIPDDRPLMEGDI
                     VSVDCGVRLKGYCGDAARTFAVGRISPESRKLLDVTQQMLALALSEIRPGVRWGTIAR
                     KMQKYVEGEGFGVVREFVGHGIGREMHEEPKVPNAWGRGLKNMDFELVPGMILAIEPM
                     VTAGSPEVEFGDDAYRWAVVTKDRRPAAHFEDTVAVTANGPDVLTDGR"
     gene            1283..1396
                     /gene="rpmJ"
                     /locus_tag="PLU99_15050"
     CDS             1283..1396
                     /gene="rpmJ"
                     /locus_tag="PLU99_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOO18410.1"
                     /translation="MKVRSSVKRICENCKIVKRKGVVRVICTNPRHKQRQG"
     gene            1416..1805
                     /gene="rpsM"
                     /locus_tag="PLU99_15055"
     CDS             1416..1805
                     /gene="rpsM"
                     /locus_tag="PLU99_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOO18411.1"
                     /translation="MARIAGVDIPSDKRTDIALQRIYGVGPFRAREICAKAQIDPAIR
                     AGKLTEDQVSRIANLIESDYVVEGQLRRQVQQNIARLRDIRCYRGLRHMRGLPVRGQR
                     TKTNARTRKGPRKTVAGKKGIKEMHRG"
     gene            1827..2234
                     /gene="rpsK"
                     /locus_tag="PLU99_15060"
     CDS             1827..2234
                     /gene="rpsK"
                     /locus_tag="PLU99_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008696448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOO18412.1"
                     /translation="MAKATGAKRRQKRSVAKAIAHVRASFNNTIITLTDMNGDVLCWA
                     SSGSVPLGGGEKKFSGARKSTPFAAQRAAQYVANEARKFGVLEVEVRVKGPGSGRESA
                     ITALQAAGLRIQSIEDVTPLPHNGCRPRKKRRV"
     gene            2238..2879
                     /gene="rpsD"
                     /locus_tag="PLU99_15065"
     CDS             2238..2879
                     /gene="rpsD"
                     /locus_tag="PLU99_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOO18413.1"
                     /translation="MSRYTGPSTRLSRREGVNLMLKPIRDESGKNPLEREYKNYPPGM
                     HMWRRGRSSEYAVRLREKQKVKRHYGILERQFMRYYDLAARSPGNTGEELLRILERRL
                     DNVVYKTRFIAGRRGARQAIVHGHILVNGRKVDKPGYLVEVGDRIAVHSRERSRKYAQ
                     SQMQLLEGVPNPPPQSWLQVDPAKLEATVLALPSREDVLIPVEEQLIVEFASR"
BASE COUNT          596 a          893 c         1044 g          487 t
ORIGIN      
        1 gaaccgtacc gcaggctgag gctctggacg ccggcttgaa ggccagtggg cggaccttgc
       61 aggctgtaat agatttccgc ctcgacgacg cggagatcat ccgccgcatc attacccgcc
      121 gggtttgcat cgattgcggg gcggcctata atacggagtt catgccgccg aaaaaggcgg
      181 gtgtatgtga tcggtgtaac ggggaagtta ttcagcgggt tgatgaccgc gaagagacgg
      241 ttcggacgcg gctggccacg taccgcgcgc agaccgagcc cctgatcggg tactacaccc
      301 ggcagggcct gctgcagtct gtggacgcga gcgtgggggc ggacgccgtt caaaaagaag
      361 tgttgcggat cgtggaggcg ctaggtacgc gcggatgaag gcggccgggg ccagaatagt
      421 tttgaaatcg gcccgcgaaa tcgaactgat gcgggcggcc ggtcgcctgg tatacgagat
      481 tctgtgcgag ctgcagcggc ggatccggcc gggcgtgacc accgccgaac tggacagagt
      541 cgccggccag atgattcgcg atgccggcgc gacggcgttg ttcctgggcg tgcgaaatcc
      601 gcaggcccgt tgcccgtttc cggcgagcat ttgcgcgtcg gtcaacgagg cggtggtaca
      661 cggtatcccg gacgaccgcc cgctgatgga aggcgacatc gtcagcgtgg attgcggcgt
      721 gcggctgaaa ggctattgcg gcgacgccgc ccgcactttc gcggtgggcc ggatttcgcc
      781 ggagtcccgg aagctgctgg atgtgacgca gcagatgctg gcgctggccc tgagcgagat
      841 tcgccccggc gtacgctggg gcacgatcgc ccgcaagatg cagaaatacg tcgagggcga
      901 aggcttcggc gtagtgcgtg agtttgtcgg ccacggcatc ggacgcgaga tgcacgaaga
      961 gcccaaggtg cccaacgcct ggggccgcgg cctgaagaat atggatttcg agttggtgcc
     1021 gggcatgatc ctggcaatcg agccgatggt caccgccggc agcccggagg tggagttcgg
     1081 cgacgacgcg tatcgctggg cagtggtcac gaaggaccgc cggccggcgg cgcattttga
     1141 ggacaccgtg gcggttacgg ccaacggccc ggatgtcctg acggacggtc gttgaggact
     1201 gtttgggacc ggcctttgta agaccggtca aagtcggacc ggttcaaaac cggtcagccg
     1261 ccagcggcgg tgcgaggttg ccatgaaggt acgcagcagc gtcaaacgca tttgtgagaa
     1321 ctgcaaaatc gttaagcgca agggcgtggt gcgagtgatc tgcaccaacc cgcgccataa
     1381 gcagcgccaa gggtagttag agaaggcgga acggtatggc tcgtatcgcg ggtgttgaca
     1441 ttcccagtga caaacggacg gacatcgctt tgcagcggat ttacggcgtc gggccgttcc
     1501 gggcgcggga aatctgcgcc aaggctcaga tcgatccggc catccgcgcc ggcaagctca
     1561 ctgaagatca ggtcagccgc atcgccaacc tgatcgaaag cgactacgtc gtcgaaggtc
     1621 agttgcggcg gcaggtgcag cagaacatcg cgcggctgcg cgatatccgc tgttaccgcg
     1681 ggctgcgcca catgcgtggc cttccggtgc gcgggcagcg caccaagacg aacgcccgga
     1741 cgcgcaaggg gccgcgcaag acggtggccg gcaagaaggg tatcaaggaa atgcatcgcg
     1801 gttaacgcat cgcaggagac cggaacttgg ccaaggcaac aggcgccaaa agacgtcaga
     1861 aacgcagcgt ggcgaaggcc atcgcgcacg tgcgcgcttc gttcaacaat accattatca
     1921 cgctcaccga tatgaacggc gacgtgttgt gctgggcctc atcgggctca gtgccgctgg
     1981 gcggcggcga aaagaaattc tccggcgccc gcaagagcac gcccttcgcc gcgcaacgcg
     2041 ccgcccaata cgtggcgaac gaagcccgca agttcggcgt tttggaagtc gaggtgcggg
     2101 tgaagggccc cggctcgggg cgcgaatccg ccatcaccgc cctgcaggcc gccggtctgc
     2161 ggattcagtc aattgaagac gtcaccccgt tgcctcacaa cgggtgccgt ccgcgcaaga
     2221 agcgcagggt gtagcccatg agtcgatata cgggacccag cacgcggctc tcgcggcgcg
     2281 agggcgtcaa tctgatgctc aagcccatcc gcgacgagag cggcaagaac ccgctcgaac
     2341 gcgaatacaa gaattacccg cccggtatgc acatgtggcg ccgcggccgc tcctcggaat
     2401 atgcggtccg cttgcgcgaa aagcaaaagg tcaagcgcca ctacggcatc ctcgagcggc
     2461 agttcatgcg ctactacgat ctggccgccc gctcgcccgg caacaccggc gaggagctgc
     2521 tgcggatcct ggagcgccgc ctggataacg tagtctataa gacccgtttc atcgccggcc
     2581 gccgcggggc gcgccaggcc atcgtacacg ggcacatcct ggtgaacggc cgcaaggtcg
     2641 acaagccggg ttacctggtc gaggtgggcg atcgcatcgc ggtgcattcg cgcgagcggt
     2701 cgcgcaagta tgcacagagt cagatgcagt tgctcgaggg cgtgccgaac ccgccccccc
     2761 agagctggct gcaggtggac ccagccaaac tggaagccac cgtgttggcg ttgcccagcc
     2821 gtgaggacgt gctcattccg gtggaagagc agctcatcgt cgagttcgcc tcgcgctgac
     2881 gccggcaggt ttgatttgga ttagccgaga gaagacctct cgggaggcat gagcaaatgc
     2941 ggatcagatg gcggggcctg gaactgccga cacgtgtcgt gcgcgacgag gcgctcagca
     3001 cggacacgta cggcctgttc
//
LOCUS       DAOSYS010000348         2988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25861_length_2988_cov_5.633140, whole genome shotgun sequence.
ACCESSION   DAOSYS010000348 DAOSYS010000000
VERSION     DAOSYS010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25861_length_2988_cov_5.633140"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLU99_15070"
     CDS             <1..1227
                     /locus_tag="PLU99_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18414.1"
                     /translation="VGWSHKYTEVLELFDLQEYALDYALVTGPGLREMFERVEREETE
                     MRAKLADRLPGVIELSLINARLAAELLGGPRATTSEQTHREPAKAGRALKLASTLAKV
                     LVLGCCFAYLGAVLVRNFEDLQAARQTLRLWPIIASFPLAVLYLIGRGLVWHLMVRRM
                     VGRFSAGADVRCWLSSLLGKYLPGKVFALLGRLYMYQSNGVRSAQVTACFLLEAACAA
                     LAALLIAVVGMLWTWGPDRIGALAAPAALLVAGLIIATHPGVLGRLMNIGLRMAGRSP
                     IVLNVRWHDVAGWTALMAVNWLGLGLGFHLLLGALTAAPAHLYVFLTGTFAAAGIIGI
                     LAIFAPAGLGVREGVMTALLATVLPPGIAVAAALLTRLWMTLAEAGCAGVALLLGRRA
                     TAPAAAPSRELEHVPG"
     gene            1224..2582
                     /locus_tag="PLU99_15075"
     CDS             1224..2582
                     /locus_tag="PLU99_15075"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOO18415.1"
                     /translation="MSAAGGPNLSTVAAHGMCIGCGACVHADPEQRLVFNATRQMFEP
                     SAAGTAAAAAVCPGVRVDYAGLQARRFGEAPVGPLGVIEQVLLAQSTDRERNVAASSG
                     GVIKELLREYLARDEVDGAIALQHVRGLDFEPRLIRDPDDVDRLPGSIYHAVRFDGAL
                     RLLSENRGRFVLVATPCQLEGIYSYIFRCRPELAERLHATIGLLCGWQYTHHAIRAIC
                     AYHNIDFDSIEDIAFRGGGPVGRLRIRTPQREYRVHRRINFSYQVAFDRSFNIPRCHL
                     CINHTNLLADLVVGDAWLPSTVHTKTGISLVICRSKAAVQTMSRLAEQKRIVSTPVTP
                     EEVVESQTHRVAFGDFSYAYAEYLREQGAYVPELNGPNRAAARLVPRAAVEKFHRQTS
                     AKMRLQQAGRYRQLYLRKLSVEIGPLLWRYVRWFFVRVMKIKSLLGMRREVSRQQLAG
                     FE"
     gene            2712..>2988
                     /locus_tag="PLU99_15080"
     CDS             2712..>2988
                     /locus_tag="PLU99_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18416.1"
                     /translation="MSETTSNECATVARNRSAAEAKPVVGQVSAGHAAPAATPEPVAP
                     APEPTVPAAAPESAAPAITVRGVGKFYEIYARPIDRLKQTLYRGRRKF"
BASE COUNT          446 a          953 c         1050 g          539 t
ORIGIN      
        1 gtgggctggt cgcacaagta caccgaggtg ctcgagctat tcgatttgca ggaatacgcg
       61 ctcgactatg cacttgtgac cgggcccggc ttgcgtgaga tgttcgagcg cgtcgagcgc
      121 gaggaaaccg aaatgcgcgc gaagctggcc gaccggctgc cgggcgtaat cgagctttcg
      181 ctgataaatg cccgactggc ggcggagctg ctcgggggac cgcgagcgac gacctctgaa
      241 cagacgcatc gcgagccggc caaagcaggg cgggccctga agttggcctc cacgctggcc
      301 aaggtgctcg tcctgggctg ctgctttgcg tacctgggcg cggtgctggt gcgcaatttc
      361 gaggatttgc aggccgcccg gcagacgctg cgtttgtggc cgatcattgc ctcgtttccg
      421 ctggccgtgt tgtacctgat cgggcgcggc ctggtgtggc acctgatggt gcggcgcatg
      481 gtggggcgct tttcggcggg cgcggacgtg cgctgctggt tgagctcctt gctggggaag
      541 tacctgccgg gcaaggtgtt cgcgctgctg gggcggcttt acatgtacca gtccaacggc
      601 gtgcgttcgg cgcaggtgac ggcgtgcttt ctgctggagg ccgcttgcgc ggcgctggca
      661 gcgctgctga tagcggtggt cggcatgctg tggacctggg ggcccgaccg gattggagca
      721 ctggcggcgc ctgcggcgct gctggtcgcg gggctgatca tcgccacgca tcccggcgtg
      781 ctggggcggc tgatgaatat cgggcttcgc atggccggcc ggtcgccgat tgtgttgaac
      841 gtgcgctggc acgacgtggc cggttggacg gccctgatgg cggtgaactg gctcgggctg
      901 gggctgggat ttcacctgct gctgggagcc ctgacggccg cgccggctca cctttatgtc
      961 tttcttaccg gcacgtttgc ggcggccggc atcattggca ttctggcgat cttcgcgccg
     1021 gccgggctgg gagtgcgtga aggcgtgatg accgcgttgc tggcgacggt actgccgccg
     1081 ggcattgcgg tcgcggcggc actgctgacc cgcttgtgga tgacgctggc cgaggcgggc
     1141 tgtgccggcg tggcgttgct gctgggacgg cgcgcgacgg ccccggccgc ggcgcccagc
     1201 cgggaactgg agcacgtacc gggatgagcg cggcgggcgg tccgaatctc agcacggtcg
     1261 cggcgcacgg gatgtgcatc ggctgcggcg cctgcgtgca cgcagacccg gaacaaagac
     1321 tggtcttcaa cgcgacacgg cagatgttcg aaccgagcgc tgccggtacg gcggccgcgg
     1381 ccgcggtctg cccgggggtg cgcgtcgact atgccggact gcaagcgcgg cgctttggcg
     1441 aagcgcccgt cgggccgctg ggcgtgattg aacaagtact gctggcgcag agcacggacc
     1501 gggaacggaa tgtcgccgcc agttcaggcg gtgtgatcaa ggaactgctg cgcgagtacc
     1561 tggcgcgcga cgaggttgat ggcgccatcg ccctgcagca tgtgcgcggc ctggactttg
     1621 agccgcggct gatccgcgac ccggatgacg tcgaccgcct gcccggctcg atttaccacg
     1681 ccgtgcggtt tgacggtgcg cttcgactgc taagcgagaa ccgcggccgc ttcgtgctgg
     1741 tggccacgcc gtgccagctc gaaggcattt atagctacat ctttcgctgc cggccggaac
     1801 tggcggaacg cctgcacgcg accattgggt tgctctgcgg ctggcagtac acgcaccacg
     1861 cgattcgggc catctgcgcg taccacaaca tcgacttcga ttccatcgag gatattgcct
     1921 tccgtggcgg cggccccgtc ggcaggctgc gcatccgcac tccgcagcgc gagtaccgcg
     1981 ttcatcgccg gatcaacttc tcgtatcagg tggcgttcga ccgctcgttc aacatcccgc
     2041 gctgccacct ctgcatcaac catacgaacc tgctggccga cttggtggtc ggcgacgcgt
     2101 ggctgccgag cacggtgcac accaagaccg gcatcagcct cgtgatttgc cgctcgaagg
     2161 cggccgtgca gaccatgtcg cgcctggccg agcaaaaacg catcgtcagc acgccggtga
     2221 cgcccgagga ggttgtcgag tcgcagacgc atcgcgtggc cttcggcgat ttctcctacg
     2281 cctacgcgga gtacctgcgc gagcaggggg cgtacgtgcc cgaactcaac ggtcctaatc
     2341 gcgccgcggc gcgcctcgta ccccgcgccg ccgtcgagaa attccatcgg cagacgagcg
     2401 ccaagatgcg gctgcagcag gccgggcgct atcgccagct ttacctgcgt aagctgtcgg
     2461 tcgaaatcgg cccgctgctg tggcgttacg tgcgctggtt cttcgtgcgc gtcatgaaga
     2521 tcaagtcgct attgggcatg cgccgcgaag tctcacgcca gcagttggcc ggctttgagt
     2581 aagcgcggct cgcgcgccag gtccgcccgg gccagcagtg ccaagttgaa gcaagcccgc
     2641 ttttcggcga ttattagtcg gccggaggac taggcagcgt gggcaaaccg cccgcggcgc
     2701 ggcgttttgg aatgagcgaa actacaagca acgagtgcgc taccgtcgcc cgaaatcgct
     2761 ccgcggcgga ggcgaagcct gtcgtgggcc aggtttcggc cgggcacgcc gcgcctgcgg
     2821 ctacgcccga acccgtcgcg cctgcgcccg aacccaccgt gcctgcggca gcgcccgaat
     2881 ccgccgcacc ggccatcacc gtccgcggtg tcggcaaatt ttacgagata tacgcccgcc
     2941 ccattgaccg cctcaaacaa actctctacc gcgggcgccg caagttct
//
LOCUS       DAOSYS010000349         2986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_25883_length_2986_cov_4.531900, whole genome shotgun sequence.
ACCESSION   DAOSYS010000349 DAOSYS010000000
VERSION     DAOSYS010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25883_length_2986_cov_4.531900"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLU99_15085"
     CDS             complement(<1..966)
                     /locus_tag="PLU99_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOO18417.1"
                     /translation="MPRIYFDNAATTPLDPRVRAAMQPYLDGDFGNPSSLHSEGRAAR
                     AAVEQARRHVAALVNAPENGVIFTASGTEANNLALRGVCAAARGKPIHVITSAIEHPA
                     VRQVIRVLGRDGVATSVLRVSADGIVDPDELRAALRPETRLVSIMAANNVIGTLQPVR
                     ELAAVAHECGALFHTDAVQAAGHVPLALERDGIDLLSLSGHKLHGPKGVGALLVRKGV
                     SLEPLVFGGGQEHGLRSGTENVPGIIGLGEAARLCRADQAVESARLVGLREGLIEGVL
                     ASVPGAYLIGHRYRRLPGNACFGFAGQEGEGMRLLLALDEAGVAVS"
     gene            complement(992..1693)
                     /locus_tag="PLU99_15090"
     CDS             complement(992..1693)
                     /locus_tag="PLU99_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOO18418.1"
                     /translation="MKLAICAGPATTGKTSVLRHIIRKLQGAGEGVAFLKIDVQFAEE
                     DEELAREFGIPTRKVYSGELCPDHCSVMVLGEALQWAQREGAGYLLVETAGLCLRCSP
                     YVDGGLGLVVLEATSGMNLPRKIGPMLSLADVAVVTKIDRISQAEREVFRARILDAAP
                     GVRVREVNALHGIGIDPLVERIRATPDVNGELLLRGNPPVGTCTICVGKKTVGWQSHF
                     GVVRPLEQQTFYRGE"
     gene            complement(1690..2475)
                     /locus_tag="PLU99_15095"
     CDS             complement(1690..2475)
                     /locus_tag="PLU99_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO18419.1"
                     /translation="MIERITLTGGRGKDGRPESVRTVEFQMGQVVSVVGPTGSGKTTL
                     INDLELLADGSTPTQRRVLINGQPPLAEYRDDPAHNPIALITQHTNFLSDLPVRDFLR
                     IHARVRHPHDEQADELVGKTLAFANQLTGEAILQHYGMTELSGGQTRALLIADAVVIG
                     NAPIVLLDEIENAGIHRLRALELLRQYRKIFVFVTHDPYIPLLSDFRIVMAGGAMTQV
                     ISSGPAERRLAHRVRAVDDILSQFRERIRSGGQFSEEELEALA"
     gene            complement(2698..>2986)
                     /locus_tag="PLU99_15100"
     CDS             complement(2698..>2986)
                     /locus_tag="PLU99_15100"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOO18420.1"
                     /translation="EQRKLEAAYDRSRNGEPLVSAEEDYLLHGSRDAFTVTITHILNQ
                     KAGLGWTSYSHTGVPLPVYAIGRGADLFEGFYDNTDVARKLAIAMGVALNN"
BASE COUNT          541 a          984 c          930 g          531 t
ORIGIN      
        1 cgagaccgcc acgcccgcct cgtcgagcgc cagcagcagc cgcatgccct ccccttcctg
       61 tcctgcaaac ccgaaacagg cattgcccgg cagccgccgg tagcggtgcc cgatcaggta
      121 tgcgcccggc acgctcgcca gcaccccttc aatcagcccc tcgcgcaacc ctaccagtcg
      181 cgcactctcc actgcctgat ccgcacggca caaccgcgca gcctctccca ggccgattat
      241 ccccggcaca ttctcggtac ccgaacgcag accatgttct tggccgccgc caaaaaccag
      301 tggctcgagc gaaaccccct tgcgcaccag aagagcgcca acacccttgg gaccgtgcag
      361 cttgtggccc gagagcgaca acaggtcgat cccgtcccgt tcgagggcga gcggcacatg
      421 cccggccgcc tgcaccgcat cggtgtgaaa gagggccccg cactcgtgtg caacggccgc
      481 cagctcccgc acgggctgaa gagttccgat gacgttgttc gcggccatga tcgaaaccag
      541 acgcgtttcg ggccgcaggg cagcgcggag ttcgtccgga tcgacgatgc cgtccgcact
      601 gacgcgtagc accgaggtgg cgacgccgtc gcgccccaga actcggatga cctggcgaac
      661 ggcggggtgc tcgatggcgc tcgtgataac gtgaatgggc ttgccgcgcg ctgcggcgca
      721 tacgccgcgc aaggccaggt tgttggcctc ggtgccgctg gcggtgaaga tgacgccgtt
      781 ctccggagca ttcaccagcg cggcgacgtg tcgccgggcc tgttcgaccg ccgcccgcgc
      841 cgcacgccct tccgagtgca ggctggacgg gttgccaaag tcgccgtcga ggtacggctg
      901 catggcagcg cgtacgcgtg gatcgagcgg cgtggtcgcg gcattatcga agtagattct
      961 tggcatgggg agctggacct tccgaggacg tctactcacc gcggtagaac gtctgttgct
     1021 ccagcgggcg aacaacgccg aaatgcgatt gccagccgac cgtcttcttg ccgacgcaga
     1081 tagtgcaagt acccacgggc gggttaccgc gcagcaagag ttcgccattt acatccggcg
     1141 tggcacgaat gcgttccacc agcggatcaa tgccaatccc gtgcagagca ttgacctcgc
     1201 gcacgcgcac gccgggagcg gcgtcgagga tgcgggcacg gaatacctca cgctccgcct
     1261 gggagatgcg gtcgatcttc gtcaccaccg cgacatccgc caggcttagc atcggcccga
     1321 tcttgcgcgg caggttcatg ccgctcgtcg cctcgagcac caccaggccc agccccccat
     1381 cgacataggg cgaacagcgc aggcacaggc cggccgtctc gaccagcagg taaccggctc
     1441 cttcccgctg cgcccattgc aatgcctcgc ccaggaccat cacactgcaa tggtccgggc
     1501 acagttcgcc ggaatagact ttgcgcgtcg gtatgccgaa ctcgcgggcc aattcctcgt
     1561 cttcttccgc gaactgcaca tcgatcttga gaaacgccac gccctcgccc gcaccctgca
     1621 gcttgcggat gatgtggcgc agcactgagg tcttgccggt cgtggccggc ccggcacaaa
     1681 tcgcgagttt cacgccagcg cctccagctc ttcttcggaa aactgcccgc cggagcgaat
     1741 gcgctcgcgg aattgcgaga ggatgtcgtc cacggcccgc acgcggtgcg ccaggcggcg
     1801 ctcggccggg ccgctgctga tcacctgcgt catggcgccg cccgccatga cgatccggaa
     1861 gtcggacaac agcgggatgt acggatcgtg cgtaacgaag acgaagatct tgcggtattg
     1921 ccgcagcagt tccagggcgc gcagccggtg gatgcccgca ttctcgattt cgtcgagcag
     1981 cacaatgggc gcatttccga tcaccactgc gtcggcgatg agcagggcgc gggtctggcc
     2041 gccggacagt tccgtcatgc catagtgctg gaggatggcc tcgccggtga gttgattggc
     2101 gaaagccaga gtcttgccga ccagctcatc agcctgctcg tcatgcgggt gccgaacacg
     2161 ggcgtgtatc cgcagaaagt cgcgaaccgg caggtcggag aggaagttgg tgtgctgagt
     2221 aatcagggcg atcgggttgt gggccgggtc atcgcgatac tccgcgagcg gcggctgtcc
     2281 gttgataagg acccgccgtt gcgtgggcgt ggagccgtcg gccagcagct ccaggtcatt
     2341 gattagcgtg gtctttccgg agccggtggg accgacgacg ctgacgactt gccccatttg
     2401 aaattcaact gttctgacgg attccggccg gccgtccttg ccacgtccgc cggtgagtgt
     2461 gatgcgttcg atcacgccat gctcccttcc gttttgtgca ctcttattag agtgcgtttg
     2521 cactctcatg agagtgtacc aaccgaacca acacgcgtca aggagacaag cgagtattgg
     2581 cgtaaattgc gtaaagtctt tacccgtaga gagttgcgcg ccactcccga tcgacgggtg
     2641 gcatagaaat ccggataaat tccttacact cctaaaggag cgttagactg gacaggctca
     2701 gttgttcagc gccacgccca tggcgatggc cagcttgcga gcaacatcgg tgttatcgta
     2761 gaacccctcg aacaggtccg caccgcgccc gatcgcgtac accggcagcg gcacgcccgt
     2821 gtgggagtag ctggtccagc ccaggcccgc cttctgattg agaatgtgcg taatggtgac
     2881 ggtaaaggcg tcacggctgc cgtgcagtag gtagtcttcc tcggcgctga caagcggctc
     2941 gccgttgcga ctgcgatcgt aggccgcttc gagctttcgc tgctca
//
LOCUS       DAOSYS010000350         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_26652_length_2914_cov_4.126618, whole genome shotgun sequence.
ACCESSION   DAOSYS010000350 DAOSYS010000000
VERSION     DAOSYS010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26652_length_2914_cov_4.126618"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLU99_15105"
     CDS             complement(<1..513)
                     /locus_tag="PLU99_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18421.1"
                     /translation="MSAGTAGYEPRPLDLRRLRMKSRAERAHKVALSALGQATPPGCS
                     FAKWFASLPAFLGAEELRATVTAIVAARRAGRPVVLAFGGHVVKTGCAPLIIDLIERG
                     IVTALASNGSGAIHDLELAETGATSEEVADTIRDGSFGMVRETCARMSQAAERGARGG
                     GLGRALGEVLL"
     gene            complement(488..1549)
                     /gene="lpxK"
                     /locus_tag="PLU99_15110"
     CDS             complement(488..1549)
                     /gene="lpxK"
                     /locus_tag="PLU99_15110"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOO18422.1"
                     /translation="MNTGDHNPPALRRVLAPLATLYGGAVALRNRYCDTFAFATHAAR
                     LPVISVGNISVGGTGKTPLVIEIVRRLRASGRRPAILTRGYAAKRGQTADEVLEYALA
                     VPETPVVVNPDRTAGAETARLEYGADCVVLDDGFQHRWLRRDLDIVVIDALDPWGGER
                     LLPEGRLREPLHNLARADWFVISRINQAPPQRVAEIVETLRRFAPHAPISKAAVAAEG
                     LSGANGSAISPECLAQRRPLPVCGVGNPSSFLQLVQELASQACEPLVFSDHHRYGMRD
                     VERILAAAAQQRADLVVTTRKDWVKLHALWPATRNGAHVAELQRLDARLVLADARGDF
                     AARLERLFEEQCERRHSGL"
     gene            complement(1566..2498)
                     /locus_tag="PLU99_15115"
     CDS             complement(1566..2498)
                     /locus_tag="PLU99_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOO18423.1"
                     /translation="MRVLITGSSGQIGTNVALALLDRGDEVCGIDIRPNPWTKRIPTV
                     LRDLNRPTDDDFGQTGKFDVVLHLAAHAKVFELVETPRRAMENVGMAFGVLEYCRRHR
                     TPLIFGSSREVYGDIHRHVTDESQADFVVAESPYSASKIAGEAFIYSYAQCYKLPHLV
                     FRFSNVYGRYDCDLERLERVIPLFIRRIHQEEPIVVYGREKVLDFTYIDDCVNGVVRA
                     IDLLVAGKVSRETINLAYGQGATLQDLVNIIGLALQKQPKVHYEPSRSGEVTRYVADI
                     SKARRLLGYTPQTPLTAGVPLSIEWQRSIGVLKA"
     gene            complement(2715..>2914)
                     /locus_tag="PLU99_15120"
     CDS             complement(2715..>2914)
                     /locus_tag="PLU99_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOO18424.1"
                     /translation="AVDRLLEMARPDTKIIPGHGPLATSDDLRAYRQMLTTVRERVQS
                     LVKAGKTGDEVLAAKPTSDFG"
BASE COUNT          500 a         1045 c          859 g          510 t
ORIGIN      
        1 cagcagcact tcgcccaggg cgcgccccaa tccgccccca cgcgccccgc gctcggccgc
       61 ctgcgacatg cgcgcacaag tctcgcgcac catgccgaag ctgccgtcac gaatcgtgtc
      121 cgcgacctct tcgctggtcg cgcccgtttc ggccagctcc agatcgtgaa tcgcccccga
      181 accgttgctg gccagtgccg tcactatgcc gcgctcaatc agatcaataa tcagcggcgc
      241 acagccggtc ttcactacgt gccccccaaa tgccagcact accggccgcc ccgcgcgacg
      301 tgccgccact atggccgtca cggtcgcccg caactcctcg gccccgagaa atgccggcag
      361 actcgcgaac catttagcaa acgagcaccc cggcggcgtc gcctgcccga gagctgacaa
      421 cgccacctta tgggcccgct cggcccgtga cttcatgcgc agccgccgca aatccagcgg
      481 ccgcggctca taacccgctg tgccggcgct cacattgttc ctcaaacagc cgctccagcc
      541 gcgccgcaaa gtcgccccgc gcatccgcca gcacgagccg cgcatccagt cgctgcagct
      601 cggccacgtg cgcaccattt ctcgtggccg gccagagcgc gtgcagtttg acccaatctt
      661 ttcgggttgt caccaccaga tccgcccgct gttgggccgc cgcggccaaa atccgctcca
      721 cgtcgcgcat cccgtagcga tggtgatcgg agaaaaccag cggctcacag gcttgactgg
      781 ccaattcctg caccaactgt aaaaacgaac tcgggttgcc gacgccgcac acgggcagcg
      841 gccggcgctg cgccaggcat tccggcgaaa ttgcgctacc gttcgccccg ctcagccctt
      901 ccgcggcgac ggcggctttt gaaatcgggg cgtggggcgc aaaccggcgt aaggtttcca
      961 cgatctccgc cacgcgttgc gggggcgcct gattgatccg cgagatgacg aaccaatcgg
     1021 cccgcgccag gttgtgcagg ggctcccgca accgcccctc cggcagcagc cgctcgccgc
     1081 cccagggatc gagcgcgtcg atgactacga tgtccaggtc gcgcctcagc cagcgatgct
     1141 ggaaaccgtc gtcgagcacg acgcaatccg ccccatattc gagtcgcgcc gtttccgctc
     1201 ccgcggtgcg atccgggttg acaaccaccg gcgtctccgg caccgccagc gcatattcca
     1261 gcacttcgtc cgcggtctgg ccgcgcttgg ccgcgtagcc gcgtgtcagg atggcggggc
     1321 gccggccgct ggcacgtaag cggcgcacaa tctcaataac cagtggagtt ttgccggtcc
     1381 cgcccacgct gatgttgccc acactgataa cgggcaggcg ggcggcatgc gtcgcgaagg
     1441 caaacgtgtc gcaatagcga ttccgcagtg caaccgcccc tccgtacagc gtcgctaagg
     1501 gagccagaac acggcgcagc gcgggcggat tgtgatcgcc cgtgttcatc tgcgcccttc
     1561 cgttatcacg ccttcaacac accgatcgaa cgttgccatt caattgacag cggcacgcct
     1621 gccgtcagcg gcgtctgcgg cgtatacccc agcagccgcc gggccttgct gatatccgcg
     1681 acatagcgcg tcacttcacc cgagcgcgac ggctcgtagt gtaccttggg ctgcttctga
     1741 agggccagcc caatgatgtt cacgaggtcc tgaagcgtcg ccccctgccc gtaagcgagg
     1801 ttaatggttt cgcgcgacac tttgcccgct accagcaggt caatcgcccg caccacgccg
     1861 tttacgcaat catcgatgta cgtgaaatcc agcactttct cgcgcccgta caccacgatc
     1921 ggttcttcct ggtgaatgcg ccggataaac agcggaataa cccgttccag acgctccagg
     1981 tcgcaatcat accggccgta aacgttgctg aaccggaaga ccagatgcgg cagtttgtag
     2041 cattgggcgt aggaatagat gaaagcttcg ccggcgatct tcgaagcgct gtaggggctt
     2101 tcggccacca cgaaatccgc ctggctctcg tctgtgacgt ggcggtggat gtcgccgtag
     2161 acctcgcgcg agctgccgaa gatcaggggc gtgcggtggc ggcggcagta ctcgagcacg
     2221 ccgaaggcca tgccgacgtt ctccattgcc cgccgcggcg tctcgaccag ctcgaagacc
     2281 ttggcatgcg cggccaggtg cagcaccacg tcgaacttgc cggtctggcc gaagtcgtcg
     2341 tccgtgggcc gattcaggtc gcgcagcacc gtcggaatgc gcttggtcca ggggttgggc
     2401 ctgatatcga ttccgcagac ctcgtccccg cggtccaaca gggccagcgc gacgttggta
     2461 ccgatctgtc cgctcgagcc agttatcaga acgcgcatgc acgctcctgc atttcatcgc
     2521 ctcttgggcg gcctgtcgcc tatgcctccc ccccggggca acttggtcgc ttttaagggg
     2581 ttggttctaa gccgtttcgc ccgccccgtc aaacgccggc ctaccgcccg gcgccggcgg
     2641 gccgtggact ggtcatgccc gcgtataccg cgcccacgaa caggtccggc ccgatcccac
     2701 cgtggcccca tttctcatcc aaaatcactc gtaggcttgg ccgcgaggac ctcatcgccg
     2761 gttttgccgg ctttaacgag cgactgcacg cgctcgcgca cggtggtcaa catctggcgg
     2821 tacgcacgca ggtcatccga ggtcgccaac gggccgtggc cggggatgat ctttgtgtcc
     2881 ggccgcgcca tttccagcag gcggtctacg gccg
//
LOCUS       DAOSYS010000351         2904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_26763_length_2904_cov_4.566866, whole genome shotgun sequence.
ACCESSION   DAOSYS010000351 DAOSYS010000000
VERSION     DAOSYS010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26763_length_2904_cov_4.566866"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLU99_15125"
     CDS             complement(<1..699)
                     /locus_tag="PLU99_15125"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOO18425.1"
                     /translation="MLEVDVIHIDPAYREALAACGLARVDQVLEQVNGRVAAWSRTTD
                     TTLVARAANQPGFYVKRYLYPTWIKRIRTMLRGTFFGTHRGQAEYRALEAMRAAGISA
                     VRPVAFGARRIGHFVAACFLITEEVPEASNLTTFAEHVVSGRRPLSPKARQAMIHTLA
                     DQVSHMHELGFSHGQLFWRNILVRSNPDRTPEFFFLDAAPRRWRKVASSQPWWQRELS
                     HLTVSGLPFTTRSDR"
     gene            complement(750..1958)
                     /locus_tag="PLU99_15130"
     CDS             complement(750..1958)
                     /locus_tag="PLU99_15130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOO18426.1"
                     /translation="MIGKLLPRRRDELAGDAGGILVVLPNWMGDVVLASPTLAALRAH
                     FSGSKISFLLRRYVREIVEGCGWHDELIFWPDKSARSGAARNAFDTHGDATRATGSGS
                     AYSGAARRGSASGEEQAARDLTASLRASGASTAVMLTNSFRSAWAVWRAGIPRRVGYA
                     RDWRSALLTDRLRPLKRNGQFVPSSVLPYYAALAEHLGCPVVDRALRLAASPAQEQAG
                     EALKRHYGLEDGRYALINCGAKFGASKCWLPERFAEVGRRISSELKLRPVLIGAPPEY
                     PLLQQIAAAAGPDVVCCTNPGTTLGSLKPVVAGAALMVCNDTGPRHYAIAYRIPTVTI
                     FGPTHQEWTDTGYDGEIKLQAAVDCGPCQLPSCPLDLRCMHGITADMVMQAAGRLLRQ
                     VSSRVAPVTA"
     gene            complement(2050..2546)
                     /locus_tag="PLU99_15135"
                     /pseudo
     CDS             complement(2050..2546)
                     /locus_tag="PLU99_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
     gene            complement(2546..>2904)
                     /locus_tag="PLU99_15140"
     CDS             complement(2546..>2904)
                     /locus_tag="PLU99_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOO18427.1"
                     /translation="RRELRGNEPLRETVDRLARRFKVSTLVVLRRIYDVGAMPKDRFW
                     REYEAEVERLTSTPRAAGGDFYHTTAVRVSKRFARAIVASTWEGRTPFTEAFQLLGFK
                     KMTTFRELGESLGVAL"
BASE COUNT          549 a          991 c          894 g          470 t
ORIGIN      
        1 ccggtcagac cgcgtagtga acggcagccc ggaaaccgtc aggtgcgaca gctcgcgctg
       61 ccaccagggc tgactggacg ccacttttcg ccaccgccgt ggcgcggcat cgagaaaaaa
      121 gaactccggc gtccgatcgg ggttggagcg gacgagaatg ttgcgccaaa agagctgccc
      181 gtgtgaaaac cccagctcat gcatgtgact gacctggtcg gccagcgtgt ggatcatggc
      241 ctgccgcgcc ttgggcgaaa gcggacgccg tcccgaaacc acgtgctccg caaaggtcgt
      301 caggttcgac gcctccggca cctcttcggt aatcagaaaa caggcggcca cgaagtgccc
      361 aatgcgccgg gcgccgaaag ccaccggccg cacggcggaa atgcccgcgg cgcgcatggc
      421 ctccagggcg cgatactccg cctgcccgcg gtgcgtgccg aaaaacgtgc cgcgcagcat
      481 ggtgcggatg cgtttaatcc aggtcggata gaggtagcgt ttgacgtaga agcccggctg
      541 gttcgctgcc cttgcgacca gcgtggtatc ggtggtgcgg ctccaggcgg ccacgcggcc
      601 gttcacctgc tccaaaacct ggtcgacgcg cgccaaaccg caggcggcca gggcctcacg
      661 gtaagccggg tcgatatgga tgacatcgac ttcgagcatc agcgcacacc tttgtggggc
      721 cgggttccga ccggctacgc agtcaccgct tacgccgtca ccggcgcaac ccgcgacgac
      781 acctggcgca gcagccgccc ggccgcctgc attaccatgt cggccgtaat gccgtgcatg
      841 cagcgcaggt cgagcgggca cgagggcaac tgacaagggc cgcaatcgac cgccgcctga
      901 agcttgattt caccgtcgta gccggtgtct gtccactcct ggtgagtcgg cccgaagatg
      961 gtgaccgtcg gaatgcgata ggcgatggca tagtgccgcg ggccggtgtc gttgcagacc
     1021 atcagcgccg ccccggccac aaccggcttg aggctgccca gcgtcgtacc cggattcgtg
     1081 caacagacca cgtccggtcc ggccgcggcc gcgatttgct gcagcagcgg gtactcaggc
     1141 ggcgcgccga tcagcaccgg tctcagcttc agttcgctgg aaatgcggcg gccaacttcc
     1201 gcaaagcgct ccgggagcca gcacttggac gccccaaact tcgccccgca gttgatcagg
     1261 gcatagcggc cgtcttccag cccgtagtgc cgtttcagcg cctcccccgc ctgctcctgt
     1321 gcgggcgaag ccgccagccg cagcgcacgg tcaacgaccg ggcagccaag gtgttcggcc
     1381 agcgcggcgt aatacggcag caccgagctg ggcacgaact gcccgttgcg cttgagcggt
     1441 ctcagccgat cggtcagcag cgcgctgcgc caatctcgcg cgtaccccac gcgccgcgga
     1501 atccccgccc gccaaacggc ccaggccgag cggaaggaat tggtgagcat cacggcagtc
     1561 gaggcgccgc tcgcgcgcaa gctcgcggtc agatcacgcg cggcctgctc ttcgccgcta
     1621 gcagagccgc ggcgtgccgc cccggaatac gccgaaccag accccgtcgc gcgtgtggca
     1681 tccccgtgcg tatcgaatgc gttgcgagcc gccccggaac gcgccgactt gtcgggccaa
     1741 aaaatcaact catcatgcca gccacagccc tcgacgatct cccgcacata tcgccgcagc
     1801 aaaaaactga ttttgctccc ggaaaagtgg gcgcgcaaag ccgccagcgt aggcgaagcc
     1861 agcacgacgt cgcccatcca attcggcaac acaaccagta tgccgccggc gtcgcccgcc
     1921 agttcatcac ggcgccgcgg cagcagtttg ccaatcatcg cccgctagtg taaccgcgca
     1981 accccaacgc aaaaactgag cagagccgga cccgttgcga agcggattcg tgtccgccgg
     2041 tagtacgcct catgccgatt cccccaacag gaaccgggca cgctcacggc gaagcatctg
     2101 aaacggcgtc atgcacttca cgtcgacgcc gatgcagaca ttcggaatct tgacccgatg
     2161 caccgagttc tcgggtttct cgtgcgtgac gacaacgcac cggcccgcca gcgcctgcgc
     2221 cactaggtaa tagtccgcgg tttggaggaa ggtgtttttg gcggccgggc tataagtctc
     2281 gtgcgcattg acccaggcgg aaccgtcccc agggctgtta ccgccgccga acctggtttg
     2341 aggaaaaaat cgttcggctg cgcgagcgcc caacgcgcca gttcgtcgtc gcctcccatg
     2401 agctcgtcgc cgactttttc aatactgaaa actttgcccg cttcatgttc gcgtaccagc
     2461 cagtcccaga aagcggggca caaatccatg ccataatgca ggttcttggc ggccatgaag
     2521 acgttcgcgt cgagcaagta ggccatcaga gtgcgacccc cagactttcc cccagttccc
     2581 ggaaggtggt catcttcttg aatcccagca attggaaggc ctcggtgaat ggagttcggc
     2641 cctcccatgt gctggcgact atggcgcggg cgaagcgctt gctgacgcgc accgccgtcg
     2701 tgtggtagaa atcaccgccg gcagcgcgtg gcgtgctcgt cagacgctcg acttcggcct
     2761 catactcgcg ccagaagcgg tctttcggca tagcgccgac gtcgtagatc cggcgcagga
     2821 cgacaagtgt actcaccttg aagcgccgcg ccaggcggtc caccgtttcg cgcagcggct
     2881 cgttgccgcg aagctcacgt ctga
//
LOCUS       DAOSYS010000352         2895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_26865_length_2895_cov_5.622887, whole genome shotgun sequence.
ACCESSION   DAOSYS010000352 DAOSYS010000000
VERSION     DAOSYS010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26865_length_2895_cov_5.622887"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLU99_15145"
     CDS             complement(<1..416)
                     /locus_tag="PLU99_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase"
                     /protein_id="HOO18428.1"
                     /translation="MLPRVIIHTAVSADGRIDGFTPDLGTFYGLVATWKEDVTLAGSD
                     TLLAAAQADAPATEEPLPAPDPGDTRPLLAIVDSRGRVRTHTALLAAGYWRQTIALCS
                     QATPAPHLAYLAKAGIPALVAGQKQVDLKAALAQLAA"
     gene            complement(475..1164)
                     /locus_tag="PLU99_15150"
     CDS             complement(475..1164)
                     /locus_tag="PLU99_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18429.1"
                     /translation="MPDYFILLVVSLGLLAIVSAFALAAISEKKRATAFAEFAAHSGL
                     QLAADAGEVLSHLPQFHLFKQGHSRRAKHWLRGGQGGEELWIFDYQHVTGAGKNRRTH
                     RHTVAAFPRLATGLPEFQLRPENIFHKIGAAFGYQDIDIQEHPEFSKRYLLRGPAEDA
                     IRQFFTLARVEGLMNMEPMCIEAAETALIIYPPSGRVRSEALPGFIDRVQTVRKLFAE
                     KTVSGVRYRLT"
     gene            complement(1324..>2895)
                     /locus_tag="PLU99_15155"
     CDS             complement(1324..>2895)
                     /locus_tag="PLU99_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18430.1"
                     /translation="FAGLGTYERPVNTSSPVAQLYFNQGLVLAYAFNHDEAIRSFKEA
                     ARLDPNFAMAWWGIALANGPHINNPHMSPAQSQAAWDALKRALALQWQAKPVDRALIQ
                     ALASRYADPPPENRRPLDEAYAAAMREVWLAYPYDPDVSTLFAESLMDLRPWDLWTTD
                     GRPRPETVEVVEVLEQTLQQAPDHPGANHLYVHAVEASPEPGKALASADRLTRLVPGA
                     GHLVHMPAHIYARTGRWQQAAEANERAIAADQQYRAVSPQQAFYRVYMAHNHHFLAFT
                     AMMEGRSEVALAAAREMIADIPPDFVESHTAHVDPLMPVPLQVLMRFGRWQEILREPA
                     PPEYMPVATAYWRFARASAYAALGQLDQAVQEQAAFRQAAAEVPPETMLMINPASHML
                     AIADHVLAGEIAFRRKDYDTAIAELRTAVELEDKLIYMEPPEWIQPVRQSLGAVLLAA
                     GRPAEAEAVYREDLQRWPNNGWSLYGLAQALRRRGDEAQAAQVQQQLEQAWSRADYAL
                     TASCSCAAKHPAGPR"
BASE COUNT          476 a          891 c         1016 g          512 t
ORIGIN      
        1 gcggccaatt gcgccagcgc ggccttgagg tccacttgct tttgtcccgc caccagcgcc
       61 ggaatgccgg ctttcgccag gtatgccaag tgcggggccg gcgtggcctg cgagcacagg
      121 gcaatggtct ggcgccagta gcccgcggct aacaacgccg tgtgagtgcg gacccttccg
      181 cggctgtcca cgatcgccag cagcggacgc gtgtcgccgg ggtccggcgc gggcaacggt
      241 tcttccgtgg caggggcgtc cgcctgcgcg gccgccaaca gggtgtcgct cccggccagc
      301 gtcacatctt ccttccaggt cgcgaccaga ccgtaaaacg tgccgagatc cggtgtgaaa
      361 ccgtcgatgc ggccgtcggc gctcacggcc gtgtgaatga ttactcgggg tagcatcttt
      421 gctccatttg tgaaagagga gccggccaag acgaaggtgc gacttggcgg gtcactacgt
      481 caggcggtaa cgcacgccgc tcaccgtttt ttcggcgaat agtttgcgga cggtctggac
      541 gcgatctatg aaacctggca gggcttcgga tctgacgcgt ccggacggcg ggtagatgat
      601 gagggcggtc tcggccgcct cgatgcacat cggctccatg ttcatcaacc cttccacgcg
      661 cgccagcgta aagaattgcc gaattgcgtc ttcggccggt ccacggagca ggtagcgctt
      721 ggagaactcc gggtgttcct ggatgtctat gtcctgatag ccgaaagcgg cgccgatctt
      781 gtggaagatg ttctcgggac gaagctggaa ttcgggcaga ccggttgcca gccgcggaaa
      841 ggcagccacg gtgtgccggt gcgtgcgccg gttcttgccc gccccggtga cgtgttggta
      901 atcgaagatc cacagctcct cgccgccctg gcccccgcgc agccaatgct tggcgcgccg
      961 gctgtgaccc tgtttgaaga ggtgaaattg cggcaggtgc gacagcacct cgcccgcgtc
     1021 cgcagccagt tgcagaccgg aatgcgccgc aaactctgcg aaggccgtgg cacgcttctt
     1081 ttcggaaatc gcggccagcg cgaaggccga tacgattgcc agcaggccca atgagactac
     1141 gaggagaatg aaataatcag gcatcgacag gtcccttccg ccgcggcggc tcgtaggctc
     1201 cccggtgact catcgtccgg cagtatcgcg ctcctggcgc cgggaggcaa gaaccggaat
     1261 attgggcgaa cgccggcgcc ccaagcaaga acgcggcgca aacgccgcgg ctatggcagt
     1321 cgattagcga gggccggcag ggtgctttgc tgcgcacgag cagctcgcag tcagcgcata
     1381 atcggcgcgc gaccacgcct gctccaactg ctgttggact tgggctgcct gggcttcgtc
     1441 tccgcgccgc cgtaacgctt gcgccaggcc gtatagcgac cagccgttgt tcggccagcg
     1501 ctgcaagtcc tcgcggtaga ccgcttcagc ctccgccggg cgcccggcgg ccaggaggac
     1561 ggcgcccagc gactggcgga cgggctgtat ccactccggc ggctccatgt agataagctt
     1621 gtcttcgagt tcgacggcag tgcgtagctc ggcgatggct gtgtcgtagt ctttgcgacg
     1681 aaaggcgatt tcgccggcca gcacatggtc ggcgatggcc agcatgtggc tggccggatt
     1741 tatcatcaac atcgtctccg gcggcacttc ggcggcggcc tggcggaagg cggcctgctc
     1801 ctgaacggcc tggtcgagtt ggccaagcgc ggcatacgca gaggcccgcg cgaagcgcca
     1861 gtatgcggtt gcgaccggca tgtattcagg cggcgccggc tcacgcagta tttcctgcca
     1921 gcggccgaag cgcatcagca cttgcagagg tacgggcatg agcggatcga cgtgcgccgt
     1981 gtgcgattcg acgaaatcgg gcgggatgtc cgcgatcatc tcgcgagctg cggcgagcgc
     2041 cacctcgctg cggccttcca tcatggccgt aaaggcgagg aagtgatggt tgtgggccat
     2101 gtaaacgcgg tagaaggcct gctgcggcga aacggcgcgg tactgctgat cggcggcgat
     2161 ggcccgctcg ttagcttcag ccgcctgctg ccagcgcccg gtgcgggcgt aaatgtgggc
     2221 cggcatgtgg accagatgcc cggcgcccgg cacgagtctg gtcagccggt cggccgaagc
     2281 cagagccttg cccggttcgg gcgaggcttc gacggcgtgg acgtagaggt ggttcgcgcc
     2341 ggggtgatcg ggggcttgct gcagagtttg ctccaggacc tcgacgactt cgactgtttc
     2401 cgggcgcggc cggccatcgg tggtccagag gtcccagggg cgcaagtcca tcaacgactc
     2461 ggcgaacagc gtgctgacgt cggggtcgta agggtaggcc agccacactt cgcgcatggc
     2521 ggcggcgtaa gcttcgtcga gcgggcggcg gttctcaggc ggcgggtcgg cgtaacggct
     2581 ggcgagcgcc tggatgagcg cgcggtccac gggctttgcc tgccactgca gcgcgagcgc
     2641 cctcttaagc gcgtcccagg ccgcctgcga ctgggccggg ctcatgtgcg ggttgttaat
     2701 gtgcgggccg ttggccaggg cgatgcccca ccaggccatg gcgaagttcg ggtcgagccg
     2761 cgcggcctcc ttgaatgagc ggatggcctc atcgtggttg aaggcgtacg caagtaccag
     2821 gccctggttg aaataaagct gggcgacggg tgacgaagtg ttcaccgggc gttcgtacgt
     2881 accgaggccg gcgaa
//
LOCUS       DAOSYS010000353         2817 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_27814_length_2817_cov_5.061550, whole genome shotgun sequence.
ACCESSION   DAOSYS010000353 DAOSYS010000000
VERSION     DAOSYS010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27814_length_2817_cov_5.061550"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLU99_15160"
     CDS             <1..1008
                     /locus_tag="PLU99_15160"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOO18431.1"
                     /translation="GAMSPFSDQQRGGSPHVESDMDLRSREHLESEADRPRHRLIGIS
                     LLPAAATLGNLLCGFLAIFACLLSVRAEYSAHLGQYAIQPRVFHPGLEQLFPTHIAAG
                     AYLILLAMVFDALDGRLARITRRTSEFGAQLDSIADIVSFGVAPMMLFITLLLRPASG
                     SVPPEVSPLQLQVGMACALVYVTCAAIRLARYNAENVRTEAAMKKFSGLPSPGAAAAV
                     VSLLALHEHLRDVNYYGLGVDWADVSRWAIAVTAFVVGLLMVSRLDYVHVFNVYARRR
                     YPPTRLVWVIAILGLAWYSLALLLVVLAFGYVLSGLIYNARRRWGRHQAAPAPGPTVE
                     S"
     gene            1008..1850
                     /gene="larE"
                     /locus_tag="PLU99_15165"
     CDS             1008..1850
                     /gene="larE"
                     /locus_tag="PLU99_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOO18432.1"
                     /translation="MQSTVAEKYEALQARLRELGSVVVAFSGGVDSALLLQVAVRTLG
                     PRHVLAVTGRSPSVPTEELQTVQALAAECGAPHEFIDTREFEDPNYTSNPTNRCYYCK
                     TELFERLWPLAAERGFRAVISGANADDAFDYRPGLQAAAERHVISPLAEAGISKRELR
                     EIAAELGLSIYDKPASPCLASRVQYGQTITPEKLARVDAAEQFLRALGFRECRVRHHE
                     HLARIEVPPGELSRFADAELRGRVDERLRELGFQYVTLDLRGFRSGSMNEVILGAGLR
                     GRAK"
     gene            1907..2293
                     /locus_tag="PLU99_15170"
     CDS             1907..2293
                     /locus_tag="PLU99_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOO18433.1"
                     /translation="MKCTHIALQVRDIDRTIAFYERYCGMRVIHERKNAFRVVWLGWG
                     EDPPRFALVLLGKDYEENRPPEYQHIGMAVDSRAEVDEIAARASGDGLEVIWPPQDAG
                     PIVGYFCGLHDPDGNMIEFSYGQRLG"
     gene            complement(2453..>2817)
                     /locus_tag="PLU99_15175"
     CDS             complement(2453..>2817)
                     /locus_tag="PLU99_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18434.1"
                     /translation="SAVSQMDKITQMNAASAEQCASAAEELSAQAQTVKNMVANLVKV
                     VGGSSARHASQKQAVPTVQAAQPMRTPEPEQPAARPQQRPAKTQDWRPAPQERPVASA
                     SRAGSADSKFELDNSAGF"
BASE COUNT          430 a          818 c         1001 g          568 t
ORIGIN      
        1 ggtgccatga gcccgttttc ggatcagcag cgtggaggtt cgccgcacgt agaatcggat
       61 atggatttgc gttcgcgcga acatctggaa tccgaggcgg accggccgcg gcaccgcttg
      121 atcggcatca gtctgctgcc ggcggcagcc acgctgggca atctgctgtg cgggtttctg
      181 gcgattttcg cctgcctgct ttcggtgcgg gcggagtatt cggcgcacct ggggcagtat
      241 gcgattcagc cgcgggtctt ccacccgggt ctggaacagc tctttccgac gcacatcgcg
      301 gccggggcgt acctgatcct gctggcgatg gtgttcgacg ccctggacgg gcggctggcg
      361 cggatcacgc ggcgcaccag cgagtttggc gcccaactcg attcgattgc ggacatcgta
      421 agcttcgggg tcgccccgat gatgctgttc atcacgctgc tgttgcgtcc ggcgagcggc
      481 tcagtgccgc cggaggtgtc gccgctccaa ctgcaggtgg ggatggcgtg cgcgctggtc
      541 tacgtcacgt gtgcggccat tcgcctggcg cgctacaacg ccgaaaacgt ccgcacggag
      601 gccgcgatga agaagttcag cggtctgccc agtcctgggg ctgcggcggc ggtcgtgtca
      661 ctgctggctc ttcacgagca cctgcgcgat gtcaactatt acggcttggg cgtggactgg
      721 gccgacgtgt cgcgctgggc cattgcggtg accgccttcg tggtcggtct gctgatggtc
      781 agccggctgg attacgtaca cgttttcaat gtgtatgcgc ggcgccgcta tccgccgacg
      841 cgcctggtgt gggtgatcgc gattctcgga ttggcgtggt attcgttggc gttgctgctg
      901 gtggtgctgg cgtttggcta cgtgctcagc gggttgattt acaacgccag gcgccgctgg
      961 ggtcgtcacc aggcagcgcc tgcccctgga ccgacggtcg agagctgatg caatccacag
     1021 tcgccgagaa atacgaagcg ttgcaggccc gcttgcggga actgggctcc gtcgtggtag
     1081 ccttctcggg aggcgtggac agcgcgttgt tgctgcaggt ggcggtgcgg acgctgggcc
     1141 cgcgccacgt gctggcggtc acggggcgca gcccgagtgt gccaaccgag gaactgcaga
     1201 cggttcaagc gctggcggcc gaatgcgggg cgccacacga gttcatcgac acgcgcgaat
     1261 tcgaggaccc gaattacacc agcaacccga ctaaccgctg ctattactgc aagacggaac
     1321 tgttcgagcg actctggccg ctggcggcgg agcgcggatt tcgagccgtg atcagtggcg
     1381 ccaatgcgga cgacgcgttc gattaccgcc cgggcttgca ggcggccgct gagcggcacg
     1441 tgatttcgcc cctggccgag gccggcatca gcaagcgtga actgcgcgag attgcggctg
     1501 agctggggct ttcgatctac gacaagccgg cttcgccctg tttggcctcg cgggttcagt
     1561 atggccagac gataaccccg gagaagctgg cacgggtgga cgcggccgag cagttcttgc
     1621 gcgcgttggg ctttcgcgag tgtcgcgtgc gacaccacga gcacttggcg cggattgagg
     1681 ttccgccagg cgaattgagt cgctttgcgg acgcggagct gcgcggccgg gtggacgagc
     1741 ggctgcggga gctgggtttt caatacgtga cgctggacct gcgcggcttt cggtcgggca
     1801 gcatgaatga agtgatactg ggcgcgggtt tgcgtggccg ggcgaaatag cgatttgagc
     1861 gatgccaggt agccccatga cggtgcggcg tggctgaggt tcgccgatga aatgcacgca
     1921 catagccttg caggtccgcg acatcgaccg gacgatcgcg ttttatgaac gctactgcgg
     1981 tatgcgcgtc atccacgagc gtaaaaacgc cttccgcgtg gtctggcttg gttgggggga
     2041 agatccgccg cgtttcgcgc ttgtactgtt gggcaaagac tacgaagaga accgtccgcc
     2101 cgaataccag cacatcggga tggcggtcga cagccgggcg gaggtggatg agatcgcggc
     2161 ccgggcgagt ggcgacggtc tggaggtcat ctggccgcca caggacgctg ggccgattgt
     2221 gggctatttc tgcgggctgc acgatccgga cgggaacatg atcgagttct cgtacgggca
     2281 gcggctggga tagagtggat tggcccgcag tgcaaggccc cgtttcaatg ctttagacat
     2341 cggatctatt gtataagcgc agccggtcgc cacggctact ggccgtggcg accggcacta
     2401 cgttcttgcg cgtctctaag aggctggttg cccgccgcac ggcgggcgct gcttagaagc
     2461 cggcgctgtt gtcgagttcg aatttggaat ccgccgaacc tgcccgcgaa gcactagcga
     2521 cagggcgttc ttgcggcgcg ggccgccaat cctgcgtttt ggcagggcgc tgctgcggtc
     2581 tggcggcggg ctgttccggt tctggagtcc tcatgggctg cgccgcctgg accgtcggca
     2641 cggcctgttt ctgactggca tggcgcgctg acgatccgcc gaccaccttc accaggttgg
     2701 ccaccatgtt cttgacggtt tgagcctgag cgctgagttc ctcggccgca gaggcgcact
     2761 gttcggccga ggcggcgttc atttgcgtga tcttgtccat ctgcgagacg gcgctgt
//
LOCUS       DAOSYS010000354         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_27951_length_2807_cov_5.001817, whole genome shotgun sequence.
ACCESSION   DAOSYS010000354 DAOSYS010000000
VERSION     DAOSYS010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27951_length_2807_cov_5.001817"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLU99_15180"
     CDS             <1..621
                     /locus_tag="PLU99_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOO18435.1"
                     /translation="GRPPPEREEGAVEPGPYYPEYQVGGQEVHPAREYEYSHFERDVA
                     SPQREAYWDWPDRPGLGPQRRWSVARRAPFQRPAYKWGVEQSPSGWRRISREYPVGPP
                     PETWTRPESMNLTTGGRGGYYGYGPKGYVRTDQRIREDVCDRLMGYDRLDCSDIEVTA
                     ENGEVTLSGTVASRWAKHVAEDIAADVLGVKDVHNRLRVQSPQNSR"
     gene            766..>2807
                     /locus_tag="PLU99_15185"
     CDS             766..>2807
                     /locus_tag="PLU99_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOO18436.1"
                     /translation="MSFLNAWAFWIAAAVVPALLLLYFLKLRRREEWIASTLLWKRAV
                     QDLQVNAPFQRLRKNLLLFLQLLVLVAGIFALARPMVHSSAAPEGRLVIIIDRSGSMN
                     AREGGQTRLDEAKEQAIRLVKTLNRRSQGWLSFLQFGGAAAKTQAMLIAFSDRAAVLS
                     PFTPNTGDLIDIIERIEPTDGRTNIREALDLAEAYMSPPTRITPGMEDAGSGTPVSAE
                     AAAKVVLISDGGLADVNEAVLRQGNIEMICVGKTSDNIGITALRTQRNYERPDVLEVF
                     LQVQNFSDQTQSTAVALKVDGVLADARVRNITLGPAKQREPAEGGKGEAGSGQSDTGA
                     DELDKADPSRRDAGVMSDIQDLSFELTLERSAVLEARLARDDALPADNSAYAVVPPPR
                     RLRVLVVTATNFFLDSVLSGLPLQERRFVTPAQYEAGLKSEFSRDGHSAWDVVIFDKC
                     SVKELPFGNYMFIGAVPEVEGIELGEQLEDLHSLVWWDESHPVLRHVGLDFVYVGKDA
                     VLKMPAEAEALIEGPRGPALARYARDGRHYLILSFAVEDSTWWQKPSFPVFAYNAIRY
                     LGGADAGQQAHVQPGDTLRIALPEGVAQAELTRPDGVRQTIRPDVGGTTYYGGTQQVG
                     VYKVTYEREGKDHTDAYAVNLEDAWESDIRPRAAEDLGTPQIKAGQAISTSTPEIWR"
BASE COUNT          518 a          848 c          990 g          451 t
ORIGIN      
        1 ggccgacccc ctccagagcg tgaggagggc gccgtggaac cggggccata ctatccggag
       61 taccaggtcg gcgggcagga ggttcatccg gcgcgcgagt acgagtactc gcactttgag
      121 cgggacgtcg ccagtccgca acgcgaggcg tattgggact ggcctgatcg cccgggactc
      181 ggtccgcaac gccgctggtc cgtcgcgcga cgcgctccat ttcagcggcc ggcctacaag
      241 tggggggttg aacagtcacc gtccggctgg cggcgcatct cgcgcgaata tccggtcggc
      301 ccgccgccgg agacctggac gcgtccggaa tcaatgaacc tgacaaccgg cggcaggggc
      361 gggtactacg gctacggccc gaaaggctac gtgcgcacgg accagcgaat tcgcgaggac
      421 gtgtgtgacc ggcttatggg gtacgaccgc ctggattgca gcgatatcga agtcaccgca
      481 gagaatggcg aagtcacgct gagcggaaca gtcgcaagcc gctgggccaa gcacgtcgcc
      541 gaagacatcg cggcggacgt gctgggcgtc aaggatgttc acaatcgctt gcgggtgcag
      601 tctccgcaga acagcagata gacacgcggt ggtttgctcg agaatccgga ttgtcggcag
      661 cccgttgcga accgcgtaga aagctgttta gcacatcatc accgtgcagt acaatgcgag
      721 tggccggcct tcggagccgc cggactgggc cgctcggaga acctggtgag cttcctcaac
      781 gcctgggcat tctggatcgc ggcagcggtc gttcccgccc tgctgctcct gtacttcctc
      841 aagctgcggc ggcgcgaaga gtggatcgcc tcgacgctgc tctggaagcg ggccgtgcag
      901 gacctgcagg tcaacgcccc cttccagcgg ctgcgcaaga acctgctgct gttcctgcaa
      961 ctgctcgtgc tggtggccgg catcttcgcg ctggcgcggc cgatggttca ctccagcgcg
     1021 gcccctgaag ggcggctggt gatcatcatt gaccgctcgg gttcgatgaa cgcccgcgaa
     1081 ggcggccaga cgcgcctgga tgaggcgaag gagcaggcga tccggctggt caagacgctc
     1141 aaccgccgca gccagggctg gttgtcgttc ctgcaattcg gcggcgcggc ggcaaagacg
     1201 caggcgatgc tgatcgcgtt ctccgaccgg gcggccgtct tgtcgccatt cacgcccaac
     1261 acgggcgatc tgatagacat catcgagcgc atcgaaccga ccgacggccg gaccaatatc
     1321 cgcgaggcgc tggatttagc cgaggcctat atgtcgcctc cgacgcgcat tacgcccggc
     1381 atggaggacg cgggcagcgg aacgccggtc agcgccgaag cggccgccaa ggtggtgctg
     1441 atctctgacg gcggcttggc ggacgtgaac gaggccgtcc tgcggcaggg caatatcgaa
     1501 atgatttgcg tcggaaagac cagcgacaac atcggcatca ccgccctgcg cacgcagcga
     1561 aactacgaac gcccggacgt tctggaggtc tttctgcaag tgcagaactt cagcgatcag
     1621 acgcaatcga ctgcggtggc gctgaaggtg gacggcgtgc tggcggatgc acgcgtgcgg
     1681 aacatcacgc ttggaccggc caagcagcgc gagccggctg aaggcggcaa aggcgaagcg
     1741 gggtcgggcc aaagtgacac gggggcggac gaacttgaca aggcagaccc ctcacggcga
     1801 gacgccgggg tcatgagcga cattcaagac ctgagcttcg agctgacgct ggagcgttcg
     1861 gcggtgctgg aggcgcggct ggcgcgcgac gacgcactgc cggccgacaa ctccgcttat
     1921 gccgtcgtgc cgccgccgcg gaggctgcgt gtgctggtgg ttacggccac caacttcttt
     1981 ctggacagcg tgctgagcgg cttgccactg caggagcggc ggtttgttac gccggcgcag
     2041 tatgaagcgg ggctcaagag cgagttcagc cgcgacgggc attccgcctg ggacgttgtg
     2101 attttcgaca agtgcagcgt gaaggagctg ccgttcggca attacatgtt catcggggcg
     2161 gtcccggagg ttgagggcat cgaactcggc gagcagttgg aggatttgca ttcgctggtg
     2221 tggtgggatg aaagccatcc ggtgctgcgg cacgtgggac tcgattttgt ctatgtcggc
     2281 aaagatgcgg tgttgaagat gccggccgag gccgaggcgc tgatcgaggg cccgcgcggg
     2341 ccggcgctgg cgcgttatgc gcgcgacggg cggcattacc tgattctgtc cttcgcggtc
     2401 gaagacagca cctggtggca gaagccgagt ttcccggtgt ttgcgtacaa cgccattcgc
     2461 tatttgggcg gggcggacgc cggccaacag gcgcacgttc agccgggcga caccctgcgc
     2521 atcgcgttgc ccgaaggcgt cgctcaggcc gagctcacgc ggcccgacgg cgtgcggcag
     2581 accatccggc ccgacgtagg cggaacgacg tactacggcg gcacgcagca agtgggcgtg
     2641 tacaaggtca cgtatgaacg cgaggggaag gaccacaccg acgcgtacgc cgtcaatctc
     2701 gaagatgcct gggaaagcga catccggccg cgagcggccg aggacctggg cacgccgcag
     2761 atcaaggcgg ggcaggccat aagcaccagc acgccggaga tttggcg
//
LOCUS       DAOSYS010000355         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28044_length_2798_cov_4.683194, whole genome shotgun sequence.
ACCESSION   DAOSYS010000355 DAOSYS010000000
VERSION     DAOSYS010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28044_length_2798_cov_4.683194"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLU99_15190"
     CDS             <1..1035
                     /locus_tag="PLU99_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18437.1"
                     /translation="GSKISGDRPLSLDAGKVINVILGEARSDADTLRAAEPIKIEPGG
                     FLVARQLAGFTNNEGEYETRWFWEIHGAPPDEACCFPDGTCEMLQTFACLMQGGTPMG
                     PGSQCDMFKCPVSAEPGACCYGTADPIQCEVMDVIKCRDEYKGTWHGPGSDCEDLNGN
                     HVADVCEIISTPEQACCFDGECRMMPVEACREEKGEPQGPGSICVGDWDGDGKDETCE
                     SKWLQPPDLSRTGIDVRDMQPIALADDFLCTETNAITRIVIWGSWLHDEYPIDPANIN
                     VTLKLREDIPDPDGSGPLYSMPGKVLWSRSWRLYQQGSTFRNRAWSPLPVSRSPLITL
                     SLYHLITRPG"
     gene            complement(1079..2344)
                     /locus_tag="PLU99_15195"
     CDS             complement(1079..2344)
                     /locus_tag="PLU99_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18438.1"
                     /translation="MNLYVRLFGSGGLICRAGLLCAGMVIAIGLLPCPGAAQETGSPF
                     PAGDPRLAGAPPEMETKTVTVEAEGYNRDDAIKQALRKALEEGAGAQIASFSQTQDFV
                     LVRDTIYSRASGIVSDFRILSEREGPGGTSIVRLEARVRPSAVAAAWGEVQNVLDQIG
                     RPKIMVWIDERIDGELQEDSMVENRIEELFVKSGFDLVTRKARGAAPLPEAADVRDER
                     GATELQRIAREAGAHILIRGSANADRAGLESLYGVPAAFYNCDVQARIYATDTGRLLV
                     SESIPSTRRGVRSRHEFSPQAAREALVQATFPPAESAGRRPALAIKLYESVMEQWSTQ
                     ISFGGDIELEVRPLDFRSYLKLKQALAGIENVRSVEGDFTDNCGQFRIKATIAAQTLA
                     ELLTGKPFSDWLEVTDLKLSRIRARSVGG"
     gene            complement(2341..>2798)
                     /locus_tag="PLU99_15200"
     CDS             complement(2341..>2798)
                     /locus_tag="PLU99_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18439.1"
                     /translation="GWAIPPAAAISRSHYPDGELDAPAWATAKMTASGRYLPRENEKL
                     SQAEQAEAARLDGVDELWQGIQTLALKDGRTLSAFFAEHTELQADLEVFLSGARVVGP
                     QSTQPAGVVTVQIELPLRRLWWIVRRGLRPAEMATAAESAGRPAQKGAP"
BASE COUNT          503 a          970 c          821 g          504 t
ORIGIN      
        1 ggcagcaaga tcagcggcga ccgtccgctg agcctcgacg ccgggaaggt catcaacgtg
       61 attttgggcg aggcgcgttc ggacgccgat acgttgcgcg cggctgagcc gatcaagatc
      121 gagccgggcg gattcctggt tgcccggcaa ctggcgggtt tcaccaacaa cgagggcgag
      181 tacgaaacgc gctggttctg ggaaattcac ggcgcaccgc cggacgaagc ctgctgcttc
      241 ccagacggaa cgtgcgagat gctgcaaacc ttcgcgtgcc tgatgcaggg cggcacgccg
      301 atgggccccg gctcgcagtg cgatatgttc aagtgcccgg tcagtgcgga gccgggtgcc
      361 tgctgctatg gaacggcgga cccgatccag tgcgaggtta tggacgtaat caagtgccgc
      421 gacgagtaca agggcacttg gcacggtccc gggagcgact gcgaggatct taacggcaat
      481 catgtggccg acgtttgcga gattatctcc acgcccgagc aggcctgctg cttcgatggc
      541 gaatgtagaa tgatgcctgt tgaggcctgc cgggaggaaa aaggcgagcc gcaaggcccc
      601 ggctcaatct gcgtcggaga ctgggacggc gacggcaagg atgaaacgtg cgaatccaaa
      661 tggctccagc cgcccgattt gagcaggacc ggcatcgacg tcagggacat gcagccgata
      721 gcattggctg acgacttcct ctgcacggaa accaatgcta ttacccggat cgtgatttgg
      781 ggatcgtggt tgcacgatga gtatcccatt gatccggcga acatcaatgt cacgcttaag
      841 ctgcgcgagg acattcctga tccggacggc tccggccccc tctacagcat gccgggcaag
      901 gtgctctggt cccggtcctg gcggctctat caacagggct ccacgttccg caaccgagcg
      961 tggagccctc tgcctgtctc ccgcagtccc cttatcacct tatcacttta tcaccttatc
     1021 acccgacccg gctgattccg acgcggaact attcgccgcc cagagccgtc agactctctc
     1081 atccccccac gctcctcgcc cgaatccggc tcagcttcag gtccgttact tccagccaat
     1141 ccgagaacgg cttgcccgtc agcaattccg ccaacgtctg cgcggcgatg gtcgccttga
     1201 tccggaactg cccacagttg tcagtgaaat ccccctcgac tgaacggaca ttctcgattc
     1261 ccgccagcgc ctgcttcagt ttgaggtacg atcggaagtc cagcggccgc acctccagtt
     1321 caatatcgcc gccgaatgag atctgcgtcg accactgctc catcaccgat tcgtacagct
     1381 tgatcgccag cgccggacgc cgccctgcgg attcggccgg tggaaacgtc gcctggacca
     1441 gcgcttcacg cgccgcttgc gggctgaact catgccgcga gcgcacgccg cgccgcgtgg
     1501 aaggtattga ctcgctcacc agcagccgcc ccgtatcggt cgcataaatg cgcgcctgca
     1561 cgtcgcagtt atagaacgcc gccggcacac catagaggct ttcgaggccg gcccggtcag
     1621 cattggccga gccgcgtatg agaatgtgcg cccccgcctc gcgggcgatt cgctgcaact
     1681 cagtcgcgcc gcgctcgtcg cgtacgtcgg ccgcctcggg aagcggcgcc gcgccgcgcg
     1741 ctttccgcgt caccaggtcg aagcccgatt tgacgaacag ctcctcgatg cggttctcga
     1801 ccatcgaatc ctcttggagc tccccgtcga tgcgttcgtc gatccagacc atgatcttgg
     1861 gccgcccgat ctggtccagc acgttctgca cctcccccca tgccgccgcc accgcgctgg
     1921 gccgcacccg ggcttcgagc cgcacaatgc tcgtgccgcc cgggccttca cgctcactga
     1981 gaattcgaaa gtcgctgaca atgcccgagg cccggctgta aatcgtgtcg cgcaccagga
     2041 cgaaatcctg tgtctgcgaa aaggacgcaa tctgcgcccc cgcgccctcc tccaaagcct
     2101 tccgcaacgc ctgcttgatc gcatcgtcgc ggttgtaccc ttcggcctcg accgtaaccg
     2161 tcttcgtctc catctcgggc ggcgcacccg cgagccgcgg gtcgccggcc gggaatggag
     2221 aaccagtttc ctgagccgcg cccgggcacg gcagaagtcc gatcgcgatt accatgcctg
     2281 cgcacagcag gccggcgcgg caaatcaagc caccagaccc aaaaagcctc acatacagat
     2341 tcatggcgct cccttctgag ccggtcggcc ggcactctcg gccgcagtgg ccatttccgc
     2401 cgggcgcaaa ccgcgccgca caatccacca cagccgcctc agcggcagct cgatctgcac
     2461 cgtgactacg ccggccggct gcgtgctctg cggcccgact acccgcgccc cgctcaagaa
     2521 cacttccagg tcggcctgca actcggtgtg ctccgcgaag aacgcagata gcgtccttcc
     2581 atctttgagg gcgagagtct gtattccctg ccataattcg tctacaccat cgagccgcgc
     2641 cgcttcggcc tgctccgcct gggatagctt ctcgttctcc ctcggcagat aacggccact
     2701 ggcggtcatt ttggccgtcg cccacgccgg cgcgtcgagt tcgccatccg ggtagtgcga
     2761 gcgcgaaatc gcggccgcgg gcgggatggc ccacccgg
//
LOCUS       DAOSYS010000356         2796 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28076_length_2796_cov_3.746443, whole genome shotgun sequence.
ACCESSION   DAOSYS010000356 DAOSYS010000000
VERSION     DAOSYS010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28076_length_2796_cov_3.746443"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..427
                     /locus_tag="PLU99_15205"
     CDS             215..427
                     /locus_tag="PLU99_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18440.1"
                     /translation="MLVDFKLDTPVWWARPGVPVKTAQSLLGHKTIAMTMQVYSHVLL
                     PDQAVALAGALPRVETQTATRAATSA"
     gene            complement(613..696)
                     /locus_tag="PLU99_15210"
     tRNA            complement(613..696)
                     /locus_tag="PLU99_15210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Leu,seq:gag)
     gene            complement(809..1831)
                     /locus_tag="PLU99_15215"
     CDS             complement(809..1831)
                     /locus_tag="PLU99_15215"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOO18441.1"
                     /translation="MLTKATVIGAGAMGTVMAQVLDSNNIKVELLARPEELDDLVVAR
                     ENRRYLPGMRLAPGISPTDDPRIALSHTELIISAYPCQYLRGMWEQLGRQVPEGVPIC
                     SITKGIEVGTLKRPTQIIAEHAPRNPLAVLSGPSIAHELARCLPATVAVASEHPEIAD
                     LVQSAVGTSWFRIYTNPDVIGVELAGALKNVIALAAGILDGLRAGDNAKSALVTRGLV
                     EITRLAVAMHARPETFVGLAGVGDLITTCISPHGRNRTAGQRIGEGRPVDQVVRESVS
                     VIEGIPTTRAVLELARRCQVEMPITQAVYDVLFNAKSPLTAITELMSRPQKAETVVDK
                     PIQNSK"
     gene            complement(1921..>2796)
                     /locus_tag="PLU99_15220"
     CDS             complement(1921..>2796)
                     /locus_tag="PLU99_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOO18442.1"
                     /translation="EVTLHQPQEENGRGSLPPSAPPEPLRAPAQLAPSVGAAWLEHSR
                     LLLEAPIDRTLADLPAVLSVSAVKQLATRGPDWRAAGPDSAHTVRDFIPRLSLPKCLQ
                     AGGEIDGRELGTACHKFLQFADFAALASESAIRQEIDRLVRSARISAEQAALLPVDDL
                     AWFGATPLGRQLALRAAAAAREVPFVYACRLTASANAEATIIRGVIDCLLETAAGLVI
                     LDYKTDRVPDDAALQERINTYTLQLQLYAQAAAQIFARPVTRAVLVFIRQRYVADVTI
                     SPPPLAQLLNAATLA"
BASE COUNT          472 a          810 c         1022 g          492 t
ORIGIN      
        1 ggctggcgat agcagaagcg ggttcggcaa tctcccttgg cgtagccgcg ggtaggcctg
       61 caaccacggc ggactgcgcg gcccggtttc gcgatgacaa gacactcgga ccgatccgtc
      121 tgacgaggcg gccaggaacg cgaagcggct aatcgggctt caggctgagc tgcggaacgg
      181 gcctgatctg gcgctggggt gaacagcgct ggacgtgctg gtcgatttca agctcgatac
      241 tccggtgtgg tgggcgcggc cgggcgtgcc agtcaagacg gctcaatccc tgctcgggca
      301 caagaccatt gcgatgacca tgcaggtgta ttcgcacgtt ctcctgcccg atcaggccgt
      361 ggcattggcg ggggcgctgc ctcgggttga aactcagacg gcgactcgcg ccgcaacgtc
      421 cgcataacca cgtccggcga ttgccaaact cgcgactctg gcacgaattt gccaaactca
      481 ctcgggggga tttcttctaa ctctcggtgg ttacagcaga tacggcgaaa tgggcaaagg
      541 ctccgtaggc cgatgctcta tccagttgag ctagcggcgc tctctgaggc ggccaaaatt
      601 ggccgaccgc actgcccggg acaggatttg aacctgcacg aggttgccct caccagcccc
      661 tcaagctggc gcgtctgcca attccgccac ccgggcgcaa agcaacggcg ccggcgaagc
      721 agcgccgatg tcaggatgaa agcttagagc gcggattcgc ttgcgtcaag attgcgtcaa
      781 gattgcgcaa cgaggaacgt ctggttcgct attttgaatt ttgaatcggt ttgtcgacca
      841 cggtttccgc cttctgcggg cggctcatta gttccgtgat ggccgtcagc ggcgatttgg
      901 cgttgaagag cacgtcgtaa acggcctgcg taatcggcat ttccacctgg cagcgccgcg
      961 cgagttccag gacggcgcgc gtcgtcggga tgccctcgat gaccgaaacg ctctcacgca
     1021 caacctggtc cacgggccgg ccttcaccga tccgctggcc ggccgtgcgg ttgcggccgt
     1081 ggggcgagat gcaggtggtg atcaggtcgc cgacgccggc caggccgacg aatgtttcag
     1141 ggcgggcgtg catggcgacg gccaggcggg taatttctac caagccgcgc gtgacgaggg
     1201 cggatttggc gttatcgccg gcgcgcaggc cgtcgaggat gccggcggca agagcgatga
     1261 cgtttttcag ggcgcccgcg agttcgacgc cgattacgtc cgggttggtg tagatgcgga
     1321 accaggaggt gccgacggcg gattggacga ggtcggcgat ttccgggtgt tcactggcga
     1381 cggccaccgt cgcgggcagg cagcgggcca gctcatgcgc gatgctcggt ccggacagaa
     1441 cggccaacgg gttgcggggc gcgtgctcag caatgatctg cgtggggcgt ttgagcgtgc
     1501 cgacctcgat gcccttggtg atgctgcaga tgggcacgcc ctccgggact tgccggccga
     1561 gctgctccca catgccgcgg agatactggc aggggtaggc agagattatg agctcggtgt
     1621 gtgagagcgc gatgcgcggg tcatcggtgg ggctgatacc gggcgccagg cgcatgccgg
     1681 gcaagtagcg ccggttctcg cgcgcgacga ccaggtcatc gagttcctcg gggcgagcga
     1741 gcagttcgac tttgatgttg ttgctgtcca acacctgggc catgacggtt cccatggcgc
     1801 cggcgccgat gactgtggct ttcgtaagca tgcggatact ccggatggac acgcggaaca
     1861 cagggcacgc cagaggtccg tgccgcggcg ccgcttgtgc cacagccggg cattgtaact
     1921 tcaggcgagc gttgcggcat ttagaagctg ggccagcggc gggggcgaga tcgtgacgtc
     1981 ggccacgtag cgttgccgga tgaagacgag cacggcgcgg gtgaccggcc gcgcaaatat
     2041 ctgagcggcg gcctgggcgt agagctgcag ttgtaacgtg taggtgttga tgcgctcctg
     2101 taaggcggcg tcgtccggca cgcggtcggt cttgtagtcc agaatgacca ggccggcggc
     2161 tgtttcgagc aggcagtcga tgacgccgcg aatgatggtc gcctcggcat tggcagaggc
     2221 agtcagtcgg cacgcgtaca caaacgggac ttcacgggca gcggcggcgg cgcgcaaggc
     2281 gagttgccgg ccgagcggcg tggccccgaa ccaggcgagg tcgtccaccg gcagtagggc
     2341 ggcctgctcg gcggaaatgc gggccgatct cacgaggcgg tcgatctcct ggcgaatggc
     2401 cgattccgag gcgagggccg caaagtcagc gaactgaagg aacttgtggc aggcggtgcc
     2461 gagttcgcgg ccgtcgattt cgccgccggc ttgcaggcat ttgggcagcg agagccgggg
     2521 gatgaaatcg cgtacagtgt gtgccgagtc cgggcccgcg gcgcgccagt cggggccgcg
     2581 agtggcgagc tgtttcactg cgctgaccga cagcacggcg ggcaagtcgg ccagggtacg
     2641 gtcgatcggg gcttcgagca gcaggcggct gtgttcgagc caggcggcac caacggaggg
     2701 cgcgagttgc gctggtgcac ggaggggttc gggcggcgca cttgggggga gactcccgcg
     2761 cccgttttct tcctggggct ggtgcagcgt gacctc
//
LOCUS       DAOSYS010000357         2795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28088_length_2795_cov_3.797810, whole genome shotgun sequence.
ACCESSION   DAOSYS010000357 DAOSYS010000000
VERSION     DAOSYS010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28088_length_2795_cov_3.797810"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /gene="treY"
                     /locus_tag="PLU99_15225"
     CDS             <1..1726
                     /gene="treY"
                     /locus_tag="PLU99_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOO18443.1"
                     /translation="AGAVQGLRVDHIDGLRDPLQYLQRLRSAAPQAWIVLEKILQRGE
                     RLPETWPVAGTTGYDFLNQVGGLFVDPAAERRLTEFNRAFTGESRDFAEVAHDQKLVM
                     LQQFFDADIQRLTEMLLEVTQDSLTYRDLARRDLHNGLRELLACFSVYRTYIRAALGE
                     IAEPDAAYVEHAIEAAREHEPELDPLVWDLLRDLLLLRLTGGVEAEFVMRFQQLCVPT
                     MAKGVEDTAFYRFSRLLSLNEVGGDSGKFGTSLAEFHRYCTELHERWPETMIATSTHD
                     TKRGEDTRIRISLLSEIPELWTEAVRRWAEMNERHRRGGAPDRSSEYQLYQTLVGAWP
                     ITLERLWPVMLKAVREAKRGTSWISPSAEYEEALQAFVTGVLQDREFVVDFERFLAPL
                     LEPAYISSLSQTLIKYTAPGIPDLYQGAELWDLRLVDPDNRLPVDYELRRRMLAELEG
                     LTPEAIWRRAASGLPKLFLIKQALHLRRRRPALFARGASYQPLLAEGEKADRVVAFVR
                     GGGALTVVPRLVIGLHGEWGETRLNVPEGDWQDVFTGQSVRAGRAHVADLLARFPVAL
                     LERQGGEP"
     gene            1723..>2795
                     /gene="treZ"
                     /locus_tag="PLU99_15230"
     CDS             1723..>2795
                     /gene="treZ"
                     /locus_tag="PLU99_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018144339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOO18444.1"
                     /translation="MNTCRVWAPNAREVELDLGESRTVMTRAARGWWSADAPLGDFDY
                     AFRIDGGQPLPDPRSMWQAEGVFGKSRQVDHSRFQWTDQTWQAPPLASCVIYELHVGT
                     FTPRGTFDGVIENLDYLRDLGISFVELMPVNEFAGSRGWGYDGVALYAPHHAYGGPAG
                     LKRLVNACHERGLGVILDVVYNHTGPEGCFLPQFGPYFTERYRTPWGPAVNFDDRGSD
                     EVRQFVIQNALMWLRDYHIDGLRLDGVHAIFDQSAVHILEELAGAVRRLEAELGRHLF
                     LIAESDLNDPRLVRPPEAGGYGLDAMWSDDFHHALHTVLTGERDGYYEDFGRLADLAK
                     AYTDGFVYDGRYSAYRGRRHGRPA"
BASE COUNT          486 a          869 c          933 g          507 t
ORIGIN      
        1 agccggggcg gttcaaggcc tgcgcgtcga tcacatagac ggcctgcgcg acccgctgca
       61 gtacctgcag cgcctccgct ccgccgcacc acaggcctgg atcgtgctcg aaaagatttt
      121 gcaacgcggc gagcggctgc ccgagacctg gccggtcgcc ggcacgaccg gctacgactt
      181 tctcaatcag gtcggcggat tgttcgtcga cccggcggcc gagcggcgct tgaccgagtt
      241 taaccgggcc ttcacgggcg aatcgcggga ctttgccgaa gtggcccacg accaaaagct
      301 ggtcatgctg cagcagttct tcgatgccga cattcagcgg ctgactgaaa tgctgctcga
      361 ggtgacgcag gacagcttga cctatcgcga tcttgcccgg cgtgatctgc acaacggcct
      421 gcgcgagctg ctggcctgct tttccgtgta tcgcacatac attcgggcag cgctcggcga
      481 gattgccgag ccggacgccg cttacgtcga acacgcgatc gaagcggccc gagaacacga
      541 gccggagctg gacccgctcg tgtgggacct gttgcgcgat ttgctgcttt tgcggctgac
      601 cggcggagtc gaagccgagt tcgtcatgcg cttccagcaa ctgtgcgtgc cgaccatggc
      661 caaaggtgtg gaggacacgg ccttttaccg cttcagccgg ctgctctcac tgaatgaggt
      721 aggcggcgat tcggggaagt ttggcacatc gctggccgaa ttccaccgct actgcactga
      781 actgcacgag cgctggccgg aaacgatgat cgccacgtcc acccacgaca cgaaacgcgg
      841 cgaggacacg cgcatccgca tcagcctgct ttcggaaata cccgagcttt ggacggaggc
      901 cgtgcgccgc tgggccgaga tgaacgagcg ccaccggcgc ggcggcgcgc ccgatcgcag
      961 cagcgaatac cagctttacc agacgctggt cggggcctgg ccgatcactc tggagcgcct
     1021 gtggccggtc atgctgaagg cggtgcgcga ggccaagcgt ggaacctcgt ggataagccc
     1081 gtcggccgag tatgaagaag cgctgcaagc atttgtaacc ggcgtgctgc aagaccgcga
     1141 attcgtcgtt gatttcgagc gcttccttgc gccgctgctg gagccggcgt acatcagctc
     1201 gctgtcccag acgctcatca agtacacagc ccccggcatc cccgacctgt accaaggcgc
     1261 cgaactgtgg gatttgcgcc tggtcgatcc cgacaatcgt ctacccgtcg attacgagct
     1321 tcgacggcga atgttggcgg aattggaagg attaacgccc gaagccatct ggcgccgcgc
     1381 tgcatcggga ctgccgaagc tgttccttat caagcaggcg ctgcatctgc gacggcgtcg
     1441 tccggcactt tttgcgcgcg gcgcgagcta ccagccgctt ctggccgagg gcgagaaagc
     1501 ggatcgcgtc gtggcattcg tacgcggcgg cggagcgctg accgtggtgc cccgcctggt
     1561 aatcggcctg cacggcgaat ggggcgagac gaggctgaac gtcccggagg gcgactggca
     1621 agacgtgttc accggccagt ccgttcgcgc cggccgcgcg cacgtagccg atttgttggc
     1681 gcgttttccc gtggcgttgc tggaacggca gggaggcgaa ccatgaacac ttgccgcgtc
     1741 tgggcgccga acgcgcgcga ggtcgaactg gacttgggag agagccggac ggtgatgact
     1801 cgcgctgcgc gtggttggtg gtctgccgat gcgccgctag gcgacttcga ttatgcattc
     1861 cgcattgacg gcgggcagcc gctgcccgat ccgcgctcga tgtggcaggc ggagggcgta
     1921 ttcggcaagt cgcgtcaagt tgaccacagt cgctttcagt ggacggacca gacttggcaa
     1981 gcgccgccgt tggcttcctg cgtcatatat gagctccatg tcggcacctt cacgccgcgc
     2041 gggacgttcg atggcgtcat tgagaacctt gactacctgc gcgacctagg catcagcttc
     2101 gtagagctca tgccggtcaa cgagttcgcc ggctcgcgcg gctggggata cgacggcgtc
     2161 gcgctgtatg caccgcacca tgcctacggc gggccggcgg gtcttaaacg cttggtgaat
     2221 gcctgccacg agcgcggttt gggcgtcatc ctcgacgtgg tctacaacca tacggggccg
     2281 gagggctgct tcctcccgca gttcgggcct tacttcactg agcgctaccg cacgccttgg
     2341 gggccggcgg tgaacttcga cgaccgcggc agcgacgaag tacggcagtt cgtcatccaa
     2401 aacgccttga tgtggctgcg cgattaccac atcgacggcc tgcggctgga tggcgtgcac
     2461 gcgatattcg accagtcggc agtacacatt ctggaggaat tggcgggcgc cgtgcggcgt
     2521 ctggaggcgg agctgggcag acacttgttc ctcatcgccg aaagcgatct caacgacccg
     2581 cgcctcgtgc ggccgccaga agccggtggg tacggactgg acgcgatgtg gagcgatgat
     2641 tttcaccacg ccctgcacac ggtgcttacg ggcgaacgag acggatacta tgaggacttc
     2701 ggccggctgg cagacctggc gaaggcctac acggacgggt tcgtttacga tgggcggtac
     2761 tcggcgtatc gcgggcggcg ccacggccgg ccggc
//
LOCUS       DAOSYS010000358         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28200_length_2785_cov_4.447619, whole genome shotgun sequence.
ACCESSION   DAOSYS010000358 DAOSYS010000000
VERSION     DAOSYS010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28200_length_2785_cov_4.447619"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1625)
                     /locus_tag="PLU99_15235"
     CDS             complement(213..1625)
                     /locus_tag="PLU99_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOO18445.1"
                     /translation="MPHPTIRLEFDNVLTSAIGPVHGLTPAEINRAAVQMKPLIARVE
                     RERKAGLHRYRDLPNDRAMLEAVRAAAKRHSAGIENLVVLGIGGSALGNIALQTALNP
                     PYYNLLPAGKRRGPRLFVMDNVDPVQFANLLELLGSGLRKTLFNVISKSGETAETAAQ
                     FLIVRDLLSKRVVTRALKRQLLVTTDAQAGTFRELVEAEGYDSLPVPEGVGGRFSVLS
                     AVGLFSAQMCGINIGRLLEGAAEMSVRVAQPAAKKNPAAMLALLLHAFYVRGKRLHVM
                     MPYSFQLKDLADWYRQLWAESLGKERDLDGAPVVVGPTPIRALGATDQHSQIQLYREG
                     PNDKVIIFLQVDEFAADVKIPRSKNTPAALRYLESASLARLLNAEKEATEFALVASSR
                     PCLTIRFPRVDERTVGQFIMLWEAATTLAGALLNINPYDQPAVQTGKEFTFALMGKAG
                     YERQAREFKRFAARRGRSSV"
     gene            complement(1724..2050)
                     /locus_tag="PLU99_15240"
     CDS             complement(1724..2050)
                     /locus_tag="PLU99_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18446.1"
                     /translation="MSKRNVLIAVLALCGMLTLGFVAGTASRATPTPPYTLTPFGVPD
                     DNFFLLTDSRSSTIRLIVFQRTVREQMPGPSIVDGSLALTVLAEFDLDKLPRREQTAG
                     DSPAKP"
     gene            complement(2306..>2785)
                     /locus_tag="PLU99_15245"
     CDS             complement(2306..>2785)
                     /locus_tag="PLU99_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18447.1"
                     /translation="KGASYGAHGGYDAQRMAGKFEVSTFTKNERAAETVRTIFEEIDR
                     LRKEQPSADELEITKTYTLGSFPGQRETPQAVAADLWLIQSHDLPADYLEQLLKGVAA
                     TKAEDCTRLVEETIDPAKMVVVVVGPADELKADLEKIAPVTVVEPETGQAQEPDSGR"
BASE COUNT          496 a          914 c          865 g          510 t
ORIGIN      
        1 cagcgtgttc tccgcattcg acgaggctct aagttgcgaa ttccaggcgg cagccatcca
       61 gatcgcacta tggttcgcgg acacggatgc cggccgcaag ctgcacgaag aaacccgcgt
      121 gccgcgcgcc agcgtaatgg tctccagttg atcagccgcg caaacgcggt tgccgcggcg
      181 ctgacctccg cgcatgcaga cgtcgccggc tgctaaacgc tcgaacggcc gcgccgcgcg
      241 gcgaagcgct tgaattcccg cgcctgacgc tcgtaaccgg ccttgcccat cagagcgaag
      301 gtgaattcct tgccggtctg aacggccggc tggtcataag ggttgatatt gagcagagca
      361 cccgccagcg tcgtggccgc ctcccacagc atgatgaact ggccgacggt ccgttcatcg
      421 actctgggaa agcgaatcgt caggcagggg cgcgaactgg cgaccagggc gaactcggtg
      481 gcctcttttt ccgcattcag cagccgagcc agcgacgcac tctccaggta gcgcagcgct
      541 gccggcgtat tcttgctccg tgggattttc acgtcggccg caaattcatc cacttgcagg
      601 aagatgatca ccttatcatt cgggccttcg cggtagagct gaatttgcga gtgctggtca
      661 gtcgcaccga gggcgcggat gggcgtcggg ccgaccacga ccggggcccc atccaagtcc
      721 cgttctttgc ccaggctctc ggcccacaac tgacgatacc agtcggccag gtccttgagc
      781 tgaaagctgt acggcatcat cacgtgtagc cgcttgccgc gaacgtagaa cgcgtgcagg
      841 agcaatgcca gcatcgccgc cgggttcttc ttggccgccg gctgagcgac gcgcacgctc
      901 atctcagccg ccccttccag cagccggcca atgttgatgc cgcacatctg ggccgagaac
      961 aagccgacgg ccgaaaggac ggaaaagcgt ccgccgacgc cctccggtac cggcagcgag
     1021 tcgtatccct cagcctcgac gagttcgcga aacgtgccgg cctgcgcgtc ggtcgttacc
     1081 aggagctgcc gcttgagggc ccgcgtcacg acccgcttgc ttaacaggtc gcgcacgatg
     1141 aggaactgcg ccgcggtctc ggccgtttct ccggacttgc tgatgacgtt gaacagtgtc
     1201 ttgcgcagcc cggaaccgag cagctcaagc agattcgcga actgcaccgg atcgacgttg
     1261 tccatcacga acagccgcgg gccgcgccgc ttgccggcgg gcagcaggtt gtagtagggg
     1321 gggttaaggg cggtctgaag ggcgatgtta ccgagggccg agccgccgat gccgagcacc
     1381 accaggttct cgatgccggc actatgccgc ttggcggccg cgcgtaccgc ctcgagcatg
     1441 gcgcggtcgt tcggcagatc gcggtagcgg tgcagcccgg ccttgcgctc gcgctcgacc
     1501 cgggcaatca gcggcttcat ctgtactgcg gcccggttga tctcggccgg cgtcaggccg
     1561 tgaactgggc caatggcgga cgtcagaacg ttgtcgaact caagccgaat ggtcggatgc
     1621 ggcatgcaga agctcctcgc caaggttttc gaaaccgcga ggataccagt gcgccgcgtg
     1681 gtcggctata tggccggcgc gtcggcgcgc gacgcgccgc cgcctatggc ttggctgggc
     1741 tgtcgcctgc cgtttgctcg cggcggggta acttgtccag gtcaaactcg gcgaggaccg
     1801 ttagcgccaa actcccgtcc acgatactgg ggccgggcat ctgctctctc acggtacgtt
     1861 ggaaaacaat caagcggatg gttgatgatc gcgagtccgt tagcaaaaaa aagttgtcgt
     1921 cgggcacgcc gaacggggtc aacgtatacg gcggcgtggg ggtagcccgt gaagcggttc
     1981 ccgccacaaa acccagtgtc aacatcccgc aaagggccag cacggcaatc agaacattcc
     2041 gcttggacat agctacctcc tcggcgcgca ccgggaccgc taccagaact gctggtgggc
     2101 gcgcaatcgt caaagcctct atatacaccc gtgcgccgca gggtccgaga gggcgcaaaa
     2161 aatcacgaac cgccgcggct cgggtgtgaa cagagagact ggcgccggct gagaaccggt
     2221 tgccctcgct ggcgcttcgg gctcggatca ggtgcgcctg ccttgaaacc acaatttgtg
     2281 atttcaagtt gggctgccgc accggctatc gaccagaatc gggctcctgt gcttggcccg
     2341 tctccggctc taccaccgtc accggcgcga tcttctccaa gtccgctttc agctcatccg
     2401 ctgggccgac cactacgacg accatcttgg ccggatcgat cgtttcttcg accagccgcg
     2461 tgcaatcctc cgctttcgta gccgccacgc ccttcagtag ctgttccagg taatccgccg
     2521 gcaaatcgtg cgactggatc agccacagat cggccgcgac cgcctggggc gtctcgcgct
     2581 gccccgggaa gctgcccagc gtgtacgtct tggtaatctc cagttcatcc gccgacggct
     2641 gctccttccg cagccggtcg atttcctcga agatcgtccg gacagtttcg gccgcgcgtt
     2701 cgttcttcgt aaacgtgctg acctcgaact tgcccgccat gcgttgggcg tcatagccgc
     2761 cgtgcgcccc gtaagaggcg ccctt
//
LOCUS       DAOSYS010000359         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28400_length_2769_cov_7.501842, whole genome shotgun sequence.
ACCESSION   DAOSYS010000359 DAOSYS010000000
VERSION     DAOSYS010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28400_length_2769_cov_7.501842"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PLU99_15250"
     CDS             complement(<1..404)
                     /locus_tag="PLU99_15250"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18448.1"
                     /translation="MPQAPDSNSRQLWLVPQAGCSSSGPVAEVAIAVRSFRLYSYTVP
                     QPLAALVQPGTFLRVPYGRRARLVPGVCVAVAQREWTHTLQPVAEVIAGEALSEMLVK
                     LGLWISEYYACPPGLTLMSLAPAAARNPPLRRT"
     gene            1484..>2769
                     /locus_tag="PLU99_15255"
     CDS             1484..>2769
                     /locus_tag="PLU99_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18449.1"
                     /translation="MAGVVLLSASVLVAGVATFQPATSGGNLGAVVQQGPNATPATLT
                     GSNIAGLQSSGVNARTEKQVPANTGAFVSPTPATTIVVAPVASRLYGFDTSVGSAAPP
                     DTLGAYLMTPFGPDPSPTATDVTSVAGPCPVPGSIEFSIPCNHRRIGSGWMSWSHGYT
                     GDVYYTNGATSLTITLPVPSCAFYFYVEPNPFEQHNFTVTADDGSSASFSAHGSAGAA
                     YCGVYGTGLTSVTISCDIDFAVGEFALACACEPIYRACCYETSEEAYCELDIDIMDCI
                     ATGGRWDIDVVACEEFDPPCGEYMGACCYGAIADPGYEPWQCIGDYLQWECLYLSEYT
                     NWTGGESCEDPFFMCPGTPTYCDASGGCDEYISRVQVGDIDNSSTCDRYGDYTTIWTL
                     VVPGVQYPITVTNGNPWSSDVCAVWVDWNQDKTFND"
BASE COUNT          522 a          803 c          867 g          577 t
ORIGIN      
        1 gtgcgtctaa gcggtgggtt ccgcgctgcc gccggcgcca ggctcattag cgtcagcccc
       61 ggcgggcagg cgtagtattc gctgatccaa agtcccagct ttacgagcat ttcgctgagc
      121 gcttcgccgg caatcacctc ggccacgggc tgcaaggtgt gcgtccactc acgctgggcg
      181 acggcgacgc agacgcccgg caccaaacgc gctcgacgtc cgtaaggcac gcgcagaaaa
      241 gtgccgggtt gtacgagcgc cgcgagcggc tgggggacgg tgtagctgta aaggcggaac
      301 gagcgtacag caatagcgac ctcggcgacg gggccggaac tggagcagcc ggcctgtggc
      361 acgagccaaa gctggcggga gttggaatca ggagcttgcg gcaatgtggc ctcgctttat
      421 ggagttatgg tacatgggcg ccgtaagggg cagtaggtaa aggatacggc taaaattgga
      481 agggcgtccg tcccataacc cggtgtgtag gagggggctc gatcggtagg cttcacgggg
      541 cagtctgtct tataggacgt acgttgaggc cacttcgggg tggcctgtct gactggctgc
      601 tggcaccggc cggcgttggt tgtgcgattt gatcccacga cctcgtttct gggccggtct
      661 cggtcacggt ttatggccgc tccgagctac gccggttggt gggtcaggtg tcagcgcgtt
      721 ttcaacattt ccgatccgcg ctatagcaaa tacccaactc ttcgctcggc tgtgcggtat
      781 ttcagagtta gcgactcgca aattgtctgc ggcgcctatg tacaatctac ctaatccgtg
      841 tatacacgaa atcgcgcacg gctccacaga ttacacacat tggcaaatgt ccgtcgatat
      901 ttcagagttt ccgactcgct aaggcggctt catgcgctcg tttcctcatc aaaaactaca
      961 gacttcaagt gccccaaaac tgcacgcact cggcggactt ctctatagta tttttggagt
     1021 ccacaactct ggaactgcgt ctgtcacttc caaaaaaaac gccccccaat tccaaaaaaa
     1081 gaattgactg agggcgaatg acgcagttag aattcgattc ggagcaagga ggaaagcacc
     1141 acgtactacc ttgtggaaac cataacggtt acctgtatct ttttcacgcg gctcgtctgc
     1201 cgctagtgtg cgtattgtcg cttgagggga aagtcgatgg tcgtcgggcg taatgccgcc
     1261 ttctggcgcg gaacttgttc tgccgctcac ccaagtcgcg cctgccgccg acttcattcc
     1321 ctggtccagt ttggtgttat tcgtttttgt aggcgacacc aagtcgggac ggaggacccg
     1381 cggccaacca tttatgggag tggaggccgg gtttttcgtc agacgcagaa cgatccattt
     1441 catgtgagga ggtaagagaa tggtttgcaa gagatggtta tgtatggcag gagtcgtact
     1501 cctgtcggcc agcgtcctgg ttgcaggcgt ggccacattt cagccggcga cgtccggcgg
     1561 gaacctcggg gctgtggtgc aacaaggccc caatgccacg ccggccacgc tgacgggctc
     1621 gaacatagct ggactgcagt ccagcggtgt gaacgcccgg actgaaaagc aggtcccagc
     1681 caacacgggc gcctttgtgt ccccgacccc agccacgacg atcgtggtag cgccggtggc
     1741 tagccggctg tacgggtttg acaccagcgt cgggtcggcg gccccgccgg atacgttggg
     1801 cgcgtatctc atgaccccgt tcgggcctga tccttcgcct actgcaactg acgtgaccag
     1861 cgtggcgggc ccgtgcccgg ttcccggctc gatcgagttc agtattccgt gcaaccaccg
     1921 caggatcggt tccggctgga tgagctggag ccatgggtac acgggtgacg tgtattacac
     1981 caatggcgcc accagcctga cgatcacgct gccggtgccg agctgtgcgt tctacttcta
     2041 cgtcgagccg aatccgttcg agcagcacaa cttcaccgtt acggcggatg acggctcgtc
     2101 ggcttcgttc agcgctcatg gttcggccgg tgccgcgtat tgcggcgtgt acggcacggg
     2161 cctgacctcg gtgacgattt cgtgcgacat tgacttcgcg gttggcgaat tcgccctggc
     2221 ctgcgcgtgc gagccgatct atcgcgcttg ctgctatgag acctctgagg aggcttactg
     2281 cgagcttgac atcgacatca tggattgcat cgccacgggc gggcgctggg atatcgacgt
     2341 cgttgcgtgc gaagagttcg acccgccgtg cggcgagtac atgggcgcct gctgctacgg
     2401 cgccatcgcc gacccgggct atgagccctg gcagtgcatc ggcgactacc tgcagtggga
     2461 gtgcctctac ctgagcgagt acacgaactg gaccggcggc gagagctgcg aagatccgtt
     2521 cttcatgtgc ccgggcacgc cgacttactg cgatgcgtcc ggcggctgcg atgagtacat
     2581 ctcgcgcgtg caggtcggcg acatcgacaa cagcagcact tgcgaccggt acggcgatta
     2641 caccacgatt tggacgttgg tcgttccggg cgtgcagtac ccgatcacgg tcaccaacgg
     2701 caacccctgg tcctccgacg tgtgcgcggt ctgggtcgac tggaaccagg acaagacctt
     2761 caacgacga
//
LOCUS       DAOSYS010000360         2764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28454_length_2764_cov_3.549280, whole genome shotgun sequence.
ACCESSION   DAOSYS010000360 DAOSYS010000000
VERSION     DAOSYS010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28454_length_2764_cov_3.549280"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="PLU99_15260"
     CDS             <1..497
                     /locus_tag="PLU99_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOO18450.1"
                     /translation="PGAGYQMQLTKTLTPERIRVPMEATEKFAAIAELIAVLDSTGGL
                     TDCHAALEAVRKREAERTTGIGYGLAIPHAKSAGCKSLVVAAGKPAKPIDFQSVDGRP
                     VSFIVLLVSPPDQTGPHIQALAQISRLMEMEDFRAAVDRAKSAQELYEAIAFYEATSE
                     PAKR"
     gene            complement(519..1751)
                     /gene="lpxB"
                     /locus_tag="PLU99_15265"
     CDS             complement(519..1751)
                     /gene="lpxB"
                     /locus_tag="PLU99_15265"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOO18451.1"
                     /translation="MAKRRRVFISVCEDSADVHAAALMRCAREMLPECEFYGLTGPRM
                     RALGAETLYDFTAHAAMLSGVLSVIGHARRATRIVQQSWRERPPDLVLLLDSPELHLR
                     FARRARRLGLPVLYYIAPQTWAAREYRNRRIARDVDRLACIMPFEEEYFRKAGVSAEF
                     VGHPLFETLASARPDEARVMELRGAGGRGEPPRPGLAAASGPVLALLPGSRRHVIETL
                     LPRQLQVVRRLHERGLAVKAVISCVAPDRRPLIEEHLAAAGLPASIVEGDNATVLAAA
                     DLVLVASGTAVLEVAFHRKPMIVMYDAGRWLAWMHRLVGWFLLKLPHLSLVNILAGAR
                     VVPEFMPFIRDIGPVADVAARLIADEPWRGLMLEQIDRLVRPLEESRASERVCEIMAE
                     MLNLEPVAAGAGAISGQR"
     gene            2077..>2764
                     /locus_tag="PLU99_15270"
     CDS             2077..>2764
                     /locus_tag="PLU99_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18452.1"
                     /translation="MPRGASRRGPSLAKEISGLLMLAFLLGCQGQGEFRDLEPSIATY
                     RDRMLVEHTAQPPDVPPPTLEPEPSEPVAQPVPWQADLPPRAALVTRPEELPQPRPID
                     VLIEIPDPETAPQAFRDRLEALKSVPKPDERVIANYEQVLPRAIENLKQIGAGRTRKV
                     YLSLAEAVQRALEHNYSIRFESYTPAISRTQLIQAEAAFDAEFFLDVNVDKRDLQTTD
                     PRRPAVSQMTT"
BASE COUNT          551 a          967 c          814 g          432 t
ORIGIN      
        1 aacccggagc tggctaccaa atgcagctaa ctaaaactct gacgcccgag cgcatacgcg
       61 ttcccatgga agcaaccgag aagtttgccg ccatagccga gctcatcgcc gtgctcgata
      121 gcaccggcgg cttgacggac tgccacgccg cacttgaggc ggtccgaaag cgcgaggccg
      181 aacgaacgac cggaatcggt tacggcctcg caataccgca cgcgaagagc gctggctgta
      241 aatcactcgt agtggccgcc ggcaaacccg ccaagcccat cgacttccaa agcgtcgacg
      301 gccggcccgt ttccttcatc gtgctcctgg tcagcccgcc tgaccaaacc ggcccacata
      361 ttcaggcgct ggcccaaatc agccgcctga tggagatgga agacttccgg gccgcggttg
      421 accgggcgaa gtcggcgcag gagttgtacg aagcgatcgc tttttacgaa gcaaccagcg
      481 agcccgccaa acgctgactc aaagttggtt acacaacctt aacgctgccc tgaaatcgct
      541 cccgccccgg ccgcaaccgg ttcgagattc agcatctcgg ccataatctc gcaaacacgc
      601 tcactcgccc ggctctcctc gagcggccgc accagccggt cgatctgctc gagcatcagc
      661 ccgcgccagg gctcgtccgc gatgagccgc gccgccacgt ccgccactgg gccgatatcc
      721 cggatgaacg gcatgaattc cggtacaacc cgcgcccctg ccagaatgtt taccagtgaa
      781 agatgcggta acttcagcag aaaccacccc accagccggt gcatccacgc cagccaacgt
      841 cccgcgtcgt acatcacgat catcggcttg cgatgaaacg cgacctccaa tacggccgtt
      901 cctgaggcca ccagcaccag gtcagccgcg gccaggacag tcgcgttgtc cccctcgacg
      961 atgctggccg gtagtccggc tgcggcgagg tgttcctcga taaggggccg ccgatccggc
     1021 gcgacgcacg aaattaccgc ctttaccgcc aacccgcgtt cgtgcagccg gcgcaccacc
     1081 tgaagctgcc gcggcagaag cgtctcgatc acgtgccgcc gtgagccggg cagcagcgcc
     1141 aagaccggcc cgcttgccgc agcgaggcct gggcgaggcg gctcgccgcg gccaccggcg
     1201 cccctcaact ccatcacgcg ggcctcgtcc gggcgggcgc tggcgagcgt ctcaaacagc
     1261 gggtggccga caaactcggc gctcacgccg gccttccgaa agtactcctc ctcaaacggc
     1321 ataatgcacg ccagccggtc cacgtcgcgg gcaattcgcc ggttgcgata ttcccgcgcg
     1381 gcccaggtct gcggcgcgat gtagtacagc accggcagtc ctaaccgccg tgcccgccgc
     1441 gcgaaccgca ggtgcaattc gggcgagtcg agcaacagga cgaggtcggg cggccgctcg
     1501 cgccaggatt gctgaacgat gcgcgtggcc cgccgcgcgt gtccgataac cgaaagcacc
     1561 ccactcaaca tcgcggcgtg cgcagtgaag tcgtacaacg tttccgcccc gagcgcacgc
     1621 atccgcggcc cagtcagccc atagaactcg cactccggca gcatttctcg cgcgcaccgc
     1681 atcagggcag ccgcatgcac gtcggccgag tcctcgcaga cggaaataaa aacccgccgc
     1741 cttttcgcca tggtctaccc tgccgtggtt aacccaacct gccgcggtta acccaactct
     1801 ggtgcggtta acccaacgtg gttaacccaa tcgccctgcc gcggttagcc cacttttcgc
     1861 agataacgct tacgaccctc gctcgcggcc gcgccggggc cgattgtacc cgcaggtctg
     1921 cactacaaaa gcctcaggac gcggatacgg gttcttacat ccatcacccc acgatagcag
     1981 ccctcgggtt tgacacgtca ggaacgcaaa tctattatcg cggccgcttt ggcgcaacag
     2041 cgcggtaacg gtaaaccccg tgtcggggag acaaagatgc ctcgtggtgc gagccggcgg
     2101 gggccgtcat tggcgaagga aatcagcggc ctgctgatgt tggcgtttct cctgggctgc
     2161 cagggccagg gcgaatttcg tgatttggaa ccctcgattg cgacatatcg cgatcgaatg
     2221 ctggttgagc acacggccca gccgcccgat gtgccgccac cgacgctgga gccggagcca
     2281 tctgagccgg tcgcgcagcc ggtgccctgg caggcggacc taccgccgcg ggccgcgctg
     2341 gtcacgcgcc cggaggaact gccgcagccc agacccatcg atgtattgat cgaaatcccc
     2401 gatccggaga cggcgccgca ggccttccgg gaccgcttgg aggcgctcaa atcggtgccg
     2461 aaaccggatg agcgagtgat cgccaattac gagcaggtgc tgccgcgcgc gattgagaac
     2521 ctgaagcaaa tcggggccgg acgcacgcgt aaggtgtacc taagtctggc cgaagccgta
     2581 caacgagcgc tggagcataa ctacagcatc cgcttcgagt cgtacacccc ggccatcagc
     2641 cgaacgcagc taatccaggc ggaggccgcc ttcgatgcgg agttcttcct ggacgtgaac
     2701 gtcgacaagc gcgacttgca gaccaccgat ccacgccgtc cggcggtgtc gcagatgacc
     2761 actt
//
LOCUS       DAOSYS010000361         2741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28749_length_2741_cov_4.194341, whole genome shotgun sequence.
ACCESSION   DAOSYS010000361 DAOSYS010000000
VERSION     DAOSYS010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28749_length_2741_cov_4.194341"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="PLU99_15275"
     CDS             complement(<1..1411)
                     /locus_tag="PLU99_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18453.1"
                     /translation="MNARPTQIVLLILVSAVTAQAAVVSAWIVLAEGDPLGGSTVSTL
                     NSPFTDGAGRVGFVGALADNQRFIWWDNGPVFFSGSIPGLTGGEGTMGVSDAGGFIYS
                     PNVDGNDAVVTHGGVLLKKGDPVPPLPGLYSSFNSRPTMLPNGTAFWVGGTATSPTGS
                     SSNRHLFKAADPTDPQSITRVLGGGDVIEGKTIKTSASNFNYWISDNGEHHIHVLDMD
                     VPLNEHVYVDGAFVAQEGSPTGQGDNWSSFDIVSINNAGKYIFTGDTDGPAETDEFIA
                     FNGVIVVREGDTLDGVTLASGYTLRAASINNLNQVAHIWGSSSTEHLFFGDGAELHAS
                     VHLLGTGDGLDIDGDGQADYTVTDFAAASSVGPGLSLAENGYIYVEVEVTPVAGGPEV
                     EAIIGISLLSSGACCFHDGHCEQLSQDGCTAAGGAVWRPDVACEPNPCFQRADLNCDG
                     AVNVFDIDPFVLALTSGPGF"
     gene            complement(1780..>2741)
                     /locus_tag="PLU99_15280"
     CDS             complement(1780..>2741)
                     /locus_tag="PLU99_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOO18454.1"
                     /translation="SGPMELVTLAILGTLAIGLIAYSLWPNQSKERDAVKRRLSGGRG
                     ADESAEIREKARETATQQLVRKATPVLSKLVMPQSDQEQNNLRIKLANAGYRHPQAQM
                     VLLASKTLLAIIGLVVGVAAAVSLQQPVQMILGIGAFGAGLGFMLPNTWLSMAASGRR
                     QKLRYGLPDTLDLMVVSVESGLALDAAIKRVGEEMATVHPELSEEMRIATMETQMGIP
                     RAEALENMARRCGVDEMRSLVSVVLQAEKFGTSIAKALRNQADSIRTKRRQAAEERAQ
                     KTAVKLMIPLVLFIFPAMGVVLGGPAVLKMLQALKDHPSLGGG"
BASE COUNT          497 a          896 c          823 g          525 t
ORIGIN      
        1 cgaaacccgg gccgctggtc agggccagta cgaacgggtc gatgtcgaac acgttcaccg
       61 cgccgtcgca gttcagatcc gcgcgctgga agcacggatt cggctcgcaa gccacgtccg
      121 gacgccacac ggcgccaccg gccgcggtgc agccatcctg cgacagttgt tcgcaatgtc
      181 cgtcgtggaa gcagcaggcg ccgctcgaga gcagtgagat gcctattatg gcctcgacct
      241 ccgggccgcc ggcgaccgga gtaacctcga cctcaacata tatgtagccg ttttcggcca
      301 aagacaaacc cgggccgaca ctcgaggccg cagcgaagtc cgtgacggtg tagtcggcct
      361 gcccatcgcc atcaatgtcc aggccgtcgc cggtccccag caggtgcacc gacgcgtgca
      421 gttcggcccc gtcgccgaag aacaagtgct cggtcgaact cgagccccag atgtgcgcga
      481 cctggttgag gttgttgata ctggcggcgc gcagcgtata gccggatgcc agtgtgacac
      541 catccagcgt gtccccttcg cgcacgacga tgacgccgtt gaaggcgatg aactcgtccg
      601 tttcggccgg cccgtccgta tcgccggtga agatgtattt gccggcgttg ttaatgctga
      661 caatgtcaaa tgacgaccag ttgtcgccct ggccggtggg cgaaccttcc tgggccacga
      721 acgcgccgtc gacatagacg tgctcattca ggggcacgtc catgtcgagc acgtggatat
      781 ggtgctcccc attgtcggaa atccagtagt taaagttgga agcgctggtc ttgatcgttt
      841 ttccctcgat gacgtcgccc ccgcccaaga cacgcgtgat gctctgcgga tcggtggggt
      901 cggccgcctt gaacaggtgc cggttgctgc tcgaaccggt cggagaagta gcggtaccgc
      961 cgacccaaaa ggccgttcca ttcggcaaca tcgtgggccg gctgttgaag ctgctgtaca
     1021 agcccggcag cggtggcacg gggtcgccct tcttcagcag aacgccgccg tgcgtaacaa
     1081 cggcgtcgtt tccatccacg tttgggctgt agatgaatcc gccggcgtcg cttacgccca
     1141 tggtgccttc accgcccgtc aggccgggaa tgctgcccga gaagaacacg gggccgttat
     1201 cccaccatat gaagcgctgg ttgtccgcca gtgcgccaac gaatcccacg cgccccgcgc
     1261 cgtcggtgaa tggcgagttc agggtgctca cggtagagcc gccaaggggg tcaccctcgg
     1321 cgagcacaat ccaggcgctc actaccgccg cttgagccgt taccgccgag accagaatca
     1381 acaggacgat ttgggtaggt cttgcgttca tctcactctc ctccaagcaa ctgaatgaca
     1441 ggactttgcg caaacaggcc gccgcggcga atgccgtctg tgatcgtgat ccagcctgtc
     1501 cagtactgcc tggccctgaa gcaggtcgaa gcgtcggtct tccgccggca tttgcggcgc
     1561 agcaccgttt aacagattat gagcattcta caacttcagg ggtgagattg gtacacttat
     1621 ttgggcattt tgggcgcgct cacggcagcg cgcctatgta caggtgggga aaagacttac
     1681 gagatgtgta tccgccgaac cacgctgcgc acaagccgga actgcgtgga agcaggcagt
     1741 actgtagggc gcgcctcttt tcgaattgcc agtttggcct tagccgccgc ccagcgacgg
     1801 atggtctttg agcgcctgaa gcatcttcag cacggctggt ccgcccagca cgacgcccat
     1861 ggccgggaag ataaacaaca ccagcggaat catcagcttg accgccgtct tctgggcccg
     1921 ttcctcggcc gcctgccgcc gcttggtgcg gatcgaatcc gcttggttgc gcaacgcttt
     1981 ggcgatgctg gtgccgaact tctcagcctg caacaccacg gacaccaggc tgcgcatttc
     2041 gtcaacgccg cagcgccgcg ccatattctc cagtgcttcg gcgcgcggta tgcccatctg
     2101 cgtttccatg gtcgcaatgc gcatctcttc tgagagctcc gggtgcaccg tggccatttc
     2161 ctcgccgacg cgcttaatgg ccgcgtccag cgccagaccg gactcgaccg aaacgaccat
     2221 caaatccagc gtgtccggca gaccgtaacg cagcttctgc cgccgccccg aggccgccat
     2281 cgacagccac gtgttcggca gcatgaatcc caggcccgcc ccgaacgccc caatgcccag
     2341 gatcatttgc accggctgct gcagtgaaac cgcggccgcc acgcccacca ccaatccgat
     2401 tatggccaac aaggtcttgc tggccagcag caccatttgg gcctgcggat gccggtagcc
     2461 ggcgttggcc agcttaatgc gcaggttatt ctgctcctga tcggattgcg gcatgaccag
     2521 cttcgacagc acgggcgtgg ccttgcgcac gagctgctgt gtggccgtct cgcgcgcctt
     2581 ttcgcggatt tccgcgcttt cgtccgcgcc gcgcccgccc gacaggcggc gtttgaccgc
     2641 gtcgcgttcc ttggactggt tcggccacag gctgtaggcg atcagcccga ttgccagtgt
     2701 tcccaggatg gccagtgtga caagctccat gggaccgctc c
//
LOCUS       DAOSYS010000362         2739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_28777_length_2739_cov_4.517884, whole genome shotgun sequence.
ACCESSION   DAOSYS010000362 DAOSYS010000000
VERSION     DAOSYS010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28777_length_2739_cov_4.517884"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLU99_15285"
     CDS             complement(<1..1519)
                     /locus_tag="PLU99_15285"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOO18455.1"
                     /translation="MRRCAANHGGSLGPGQRAFTLVEMLVSLAVLSIALGVVGYVFSA
                     TINATRQAAAYSEAHNLAQQLVSQIREDLRHCDPANSLLVLVGRTQAAARTKDDLDAR
                     KFWRVLVGDPAQAPAGFDPEYGQRSALDPSMNYYSDPRADILMFFTNRPTMSQAPPTD
                     PYGGSFAEQCLQGAKFSPIQVVYGHAALDTAEYDPGTRKFKLANKPQHIEQNRPGAGT
                     GPAALSRLPLTAWHLSRRATILVDYPPNIPPQLPAHIRFTDKACQHIVACMPYIDDAV
                     PSERMPGDAAWLDWRRLLAQFGPDTEYSLSNPESWFKPYEFPEVGGGSGEGWNMDLRD
                     CIRTLMYRTGTAQPDLHHVATVVENPPLNLVSEQHSDVQSLPGNNMGVHLLPGCAWFQ
                     VEFLMPEDPRNSLYYHDPEPLGSSNHSKRWDMPRWTEVGAGRTYVFIPDTAENRDYVV
                     QLYQPTTWQPRLLDFARLDQEEDLHLSRSDAVEQRVIRMWPYAIRITVRVFDRLGR"
     gene            complement(1516..>2739)
                     /locus_tag="PLU99_15290"
     CDS             complement(1516..>2739)
                     /locus_tag="PLU99_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18456.1"
                     /translation="ALDVIEQRIRLVRDPYDETLGKPSNYADIFQPRYGEVTSLPELA
                     PGGQPGELAVEDPTAAFPNGRLWEPRIKVRPLLTNGINLTYPRVDEFEPSEGPQAAVE
                     RQIVRWFTDVIVAEAPEYLERDGDDNWTASTLSSVSAAYPPVSHESPFRASDFLASPE
                     ARYIRTQTSEAARRKLLETAVTWVAFYRRISYAEGSDPNLYEVIALATRRPQGYFYPW
                     YDSDNVLQKAVAPVPWLVVFQGVAPALRRGPDYVEIPRPEPYSPDRVLNVEFREPATL
                     TFTCDDATGYLFPPGSIFLPAVNDELLSVYAASFSPRPQFGGFIPHSPEALPIYEVTA
                     RERNAGGGWSIIVKNNGFYPWVQGDPASDLRHWPVWVIPPAFKGLDSQGKPLYDDRSP
                     VLAVARRIIRFTEVP"
BASE COUNT          521 a          837 c          871 g          510 t
ORIGIN      
        1 gtcgacccag gcggtcgaat acgcgcacgg tgatgcgaat ggcgtacggc cacatgcgaa
       61 tcacacgctg ttcaacggcg tcggagcggc taaggtgcaa gtcctcctcc tggtcgaggc
      121 gtgcaaagtc gagcaggcgc ggttgccagg tagttggctg atacagttgc accacgtagt
      181 cacgattttc ggcagtgtcc gggatgaaca cataagttcg cccggccccg acctcggtcc
      241 agcgcggcat atcccaacgc tttgaatgat tgcttgagcc caatggctcg gggtcgtggt
      301 agtacaaact gttgcgcggg tcctcgggca tcaggaactc gacctggaac catgcgcagc
      361 cgggcagcag gtgcacgccc atgttgttcc ccggcagcga ttgcacgtcc gaatgctgct
      421 cgctcaccag gttcagcggc ggattctcca ctaccgtggc gacgtggtgc aagtctggct
      481 gcgctgtccc ggttctgtac ataagcgtcc tgatacagtc gcgaaggtcc atgttccaac
      541 cctcgccgga cccgccaccc acctcgggga attcgtaggg cttaaaccag gactcaggat
      601 tgcttagcga gtactccgtg tcgggaccaa actgggccaa caggcgccgc cagtcgagcc
      661 acgcggcgtc acctggcatt ctttccgagg gaaccgcatc gtcaatatat ggcatgcacg
      721 cgacaatgtg ctggcacgcc ttgtcggtaa accggatgtg cgcaggcagt tgcggaggga
      781 tattgggtgg gtaatcgacc agaatcgtcg cgcggcggct gaggtgccat gccgtcagcg
      841 gtagcctgct taaggcggcc ggccctgtgc cggcgccggg acgattctgc tcaatgtgct
      901 gcggcttgtt cgccagttta aatttcctgg tgcccggatc gtattccgcg gtgtccagtg
      961 ccgcgtggcc gtagacgacc tggatcggcg agaacttggc accctgtaga cactgctccg
     1021 cgaacgaacc cccgtagggg tcggtgggcg gcgcctgcga catcgtcggc cgattggtaa
     1081 agaacatcag gatatcggcg cgcgggtcgc tgtagtaatt catggacgga tccagcgccg
     1141 accgctggcc atattccgga tcgaagccgg caggggcttg cgcaggatcg cccaccagca
     1201 cgcgccagaa cttcctggcg tccagatcgt ccttggtgcg cgccgcggcc tgcgtgcgcc
     1261 cgaccagcac caacaggctg ttggctggat cgcagtggcg gaggtcctct cggatctggc
     1321 tgacaagctg ctgcgcgagg ttgtgcgctt cagagtaggc cgcggcctgg cgtgtggcgt
     1381 tgatcgtggc gctgaagaca tagcccacca cgcctagagc tatggaaagc acggccagcg
     1441 agacgagcat ctcgaccagc gtgaaggcac gctggcccgg gccgagtgaa ccgccgtgat
     1501 ttgcagcgca gcgcctcatg ggacctcggt aaaacgaatg atccgccggg ctaccgccag
     1561 gacgggcgaa cgatcgtcgt aaagcggctt tccctgtgaa tccaagccct tgaacgcggg
     1621 cgggatcacc cataccggcc aatgccgcag gtcggatgcg gggtcaccct ggacccacgg
     1681 ataaaagcca ttgtttttca caatgatcga ccaacccccg ccggcgttgc gctcgcgtgc
     1741 agtgacttcg tatatcggca gcgcttccgg cgaatgcgga ataaagcctc caaactgagg
     1801 gcgggggctg aatgaggcgg cgtaaacgct gagcaactca tcattgacgg caggcagaaa
     1861 gatgctcccc ggcgggaaca ggtatccagt agcgtcgtcg catgtgaagg tcagcgtcgc
     1921 cggctctcgg aactcgacat tcaacacgcg gtccggcgaa tagggttccg gccgcggaat
     1981 ttccacataa tccgggccgc gccggagagc cggggccaca ccctgaaaga cgaccagcca
     2041 cggaaccggc gcaacggctt tctgcaggac gttgtcggag tcataccacg ggtagaagta
     2101 gccctgcggc cgtcgcgtgg ccagcgcaat cacctcatac aggttcggat cactgccttc
     2161 ggcatacgag atgcgccgat agaacgccac ccaggtcacg gccgtttcca gaagcttgcg
     2221 gcgtgccgcc tcactcgttt gcgtccggat gtaccgagcc tccgggctcg caaggaaatc
     2281 tgacgcgcgg aacgggcttt cgtgtgagac gggcggatag gcggcgctta ccgaagacag
     2341 cgttgaagcg gtccagttgt catcgccgtc gcgctccagg tactcggggg cctcggcaac
     2401 gatgacatcc gtaaaccaac gcactatctg gcgctcgacc gcggcttgag gtccttcgga
     2461 gggctcgaac tcgtctactc gcgggtaagt taagtttatg ccattcgtca gcagcggccg
     2521 gacttttatg cgtggctccc agaggcggcc gtttgggaac gccgccgttg gatcttctac
     2581 ggcaagctca cccggttggc cgcctggagc aagctccggc agggacgtga cctccccgta
     2641 tcgcggctgg aagatgtccg cataattact cggcttgcct agcgtctcgt cgtacgggtc
     2701 cctgacaagc cggatcctct gctcaatcac gtccagcgc
//
LOCUS       DAOSYS010000363         2702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_29263_length_2702_cov_4.459010, whole genome shotgun sequence.
ACCESSION   DAOSYS010000363 DAOSYS010000000
VERSION     DAOSYS010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29263_length_2702_cov_4.459010"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PLU99_15295"
     CDS             complement(<1..601)
                     /locus_tag="PLU99_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18457.1"
                     /translation="MRFHAMVGASVMSALLLGGCPVLDHQTEPGPAPVDEQAHVTPDS
                     AGDTPAPVENSPGPGPGESTLAVDPADDQTSADGSAPNGTSGSPATGEDGSEGQPAPD
                     EPLPIPPGRYSGPVFVCQTAHCTGLFATVAGPDIYNETEWPSWSAVITADGLPMLHDH
                     TLDMGGLRVDYMNGEVQITDDGVIVTWLTQVNLVNPATGE"
     gene            1044..2375
                     /gene="egtB"
                     /locus_tag="PLU99_15300"
     CDS             1044..2375
                     /gene="egtB"
                     /locus_tag="PLU99_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HOO18458.1"
                     /translation="MALRDSRTYDNHMTTTAYATSPSSALSPAADAEALRVAYEVTRR
                     ATEELCSPLEPEDYVIQSMPDASPVKWHLAHTSWFFETFVLRPHLAAYREFHPQYALL
                     FNSYYVAAGPRWPRPERGMLSRPTVADVMRYRRHIDQHMQRVLRDLPAVAAPTVLLGI
                     HHEQQHQELILTDLKHHWSLNPLQPVYSQENVPPAGDAPPLNWLEFPEQLAWIGHDGH
                     GFAFDNEAPRHREFVHAFRLASRLVTNQEYLRFMADGGYERPELWLSDGWAVRQARGW
                     TAPLYWRQQGHWTVYTLAGRRRLEPAEPVCHVSYYEADAYARWAGARLPNEAEWEVAA
                     ASAPLAGHFVESRRFHPSSAPAADDAGPLHQLYGDLWQWTASPYVGYPGYRPVTGALG
                     EYNGKFMCNQMVLRGASCATPRSHARPTYRNFFAPDARWQFAGIRLAEDRA"
     gene            2372..>2702
                     /locus_tag="PLU99_15305"
     CDS             2372..>2702
                     /locus_tag="PLU99_15305"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HOO18459.1"
                     /translation="MISQAITAAADGVRPTDEFLADVLRGLSTAPKTLPCKYLYDAAG
                     SRLFDQICALPEYYVTRAEFAILRRHGREIARCVGSSCTIIEYGSGNGRKVRLLLKHL
                     KPPLAYVP"
BASE COUNT          457 a          933 c          820 g          492 t
ORIGIN      
        1 cctcgccggt ggccggattt accaagttga cctgcgtcag ccaggtgacg atcacgccgt
       61 cgtcagttat ctgcacttca ccgttcatgt aatcgacgcg caggccgccc atgtcaagcg
      121 tgtgatcgtg gagcataggc aggccatcgg cagttatcac cgcactccaa cttggccatt
      181 cagtctcatt gtatatatcc gggccggcca cagttgcaaa caatccggta cagtgtgcag
      241 tctggcacac gaagaccggc ccgctgtacc ggccgggcgg aatcggcagc ggctcatccg
      301 gcgcaggctg gccctcactg ccgtcttcac ccgtcgcggg gctcccgctg gtcccattcg
      361 gcgccgaacc gtctgcgctc gtttggtcgt ctgccgggtc gactgccagc gtgctttctc
      421 cgggccctgg acccgggctg ttctcaaccg gtgctggagt atcgccggcg ctgtcgggcg
      481 tgacatgggc ctgctcgtcg acgggcgccg ggccgggttc agtctggtgg tccaataccg
      541 ggcagccccc cagcaacaaa gccgacatga cactggcgcc caccatggcg tgaaatctca
      601 tgatcctcgc tcctctgcgg ctctcagtcg aaccgagcct agcactttcg aagcgggcaa
      661 gtcaatcctt tctgaccggc cggccacgcg caatttcgcc gccaccgcgg cgcgcacctc
      721 caaggcccat aatttctgcc ctgcgggcgc aaacccggcc gcttgcgccg ccgcgcgcgc
      781 cgcgccggcc ggcaacgctt gcgacccata agttctcgca gacgcgcggc ttcgcgcgct
      841 cgccgcgaag gaccgttcgg actggcggag cacctgcgcg gccgcctgtc gcgaaactca
      901 gaaacattac tgccggcggc agactgaact tcgcttcagt tgaatcgcag tacaccggcg
      961 gcacttcctc cacgccgcgc agcgccgcca atccggccgc cgtgcacttg cattcagacg
     1021 cccgaaagct gacaaaaaag agaatggcgc tgcgcgacag ccgcacctac gataaccaca
     1081 tgactactac tgcctacgcc acatcgccct cgagcgcctt aagcccagcg gccgacgccg
     1141 aagcgctgcg cgtggcgtac gaggtcacgc gccgcgctac tgaagaactg tgcagtccgc
     1201 tggagcccga ggactatgtc atccagtcaa tgccggacgc cagccccgtg aagtggcatc
     1261 tggcgcacac gagttggttc ttcgaaacgt ttgtactcag gcctcacttg gcggcctatc
     1321 gcgagtttca cccgcaatat gcgctcttgt tcaattccta ttacgtagcc gcggggccac
     1381 gctggccccg cccggaacgc ggaatgctct cccggccgac ggttgcggat gtgatgcgtt
     1441 atcgccggca cattgaccag cacatgcagc gggtgctgcg cgatctgccg gcggtcgccg
     1501 cgcccaccgt gctactcggc attcatcacg agcagcaaca tcaggagctg attctcacgg
     1561 atctcaagca ccactggtcc ttgaacccgc tacaaccggt gtacagtcag gagaacgtgc
     1621 cgccggctgg cgatgccccg cccctgaact ggctggaatt cccggagcaa ctggcctgga
     1681 tcggtcatga cggccacggc ttcgcattcg acaacgaggc cccgcgccac cgcgagttcg
     1741 tgcacgcctt ccgtctcgcc agccgcttgg tgaccaatca ggaatacctg cgcttcatgg
     1801 ccgacggcgg ttatgaacgc cccgagctgt ggctttcgga cggttgggcg gtgcgccagg
     1861 cgcgcggctg gaccgcgccg ctctattggc ggcagcaggg tcactggact gtttacacac
     1921 ttgccggccg acggcgttta gagccggccg aacctgtttg ccatgtttct tattacgagg
     1981 ccgacgccta tgctcgctgg gcgggtgccc gcctgccgaa tgaggcggaa tgggaggtag
     2041 cggcggccag cgcgccgctg gcaggccatt tcgtcgagag ccggcggttt cacccgtctt
     2101 ccgcgcctgc cgccgatgac gccggcccac tgcaccagct atacggcgat ctctggcagt
     2161 ggacggccag cccatacgtc ggctaccccg gctaccggcc cgtgaccggc gccttgggcg
     2221 agtacaacgg caaattcatg tgcaatcaaa tggtcttgcg cggcgcctcc tgcgccacgc
     2281 cacgcagcca tgcccgcccc acgtatcgca acttcttcgc gcccgacgcc cgctggcaat
     2341 tcgccggcat tcggctcgcg gaggaccggg cgtgatcagc caggcgatta cggcagccgc
     2401 ggatggcgtg cggccaacgg atgagttctt ggcggatgtg ttgcgcggcc tgtcgactgc
     2461 gccgaagacg ctgccgtgca agtaccttta tgatgcggcg ggctcgcggc ttttcgatca
     2521 gatctgcgcg ttgcccgagt actacgtgac ccgtgccgaa ttcgccattc tgcgccgcca
     2581 cggacgcgag attgcacgct gcgtcggcag ttcctgcacc ataatcgagt atggcagcgg
     2641 caatggccgc aaggtgcggc tgctgctcaa gcacttgaag ccgcccctgg catacgtgcc
     2701 gg
//
LOCUS       DAOSYS010000364         2677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_29590_length_2677_cov_3.385584, whole genome shotgun sequence.
ACCESSION   DAOSYS010000364 DAOSYS010000000
VERSION     DAOSYS010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29590_length_2677_cov_3.385584"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLU99_15310"
     CDS             <1..643
                     /locus_tag="PLU99_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18460.1"
                     /translation="NSHVLDHVGIVGRFKYGVVLLGTELSSLRDCSVVLTAPGGVGLY
                     ASPENRIDFCSARLQKLIAGGTTTYNHVSGGVLGAGPGGSSVRFDGYVVAWTFTNVFF
                     GVAAEPGSESVIRLHGLQSPNRLLVPSQLTFIGCGNDQLRTRFGLYVTSERPDRVGAD
                     RLIFDNCYLLSREYPVCCKTAANDPGPETGLIRHWAERNSRFLKNGAVQEVRP"
     gene            1297..>2677
                     /locus_tag="PLU99_15315"
     CDS             1297..>2677
                     /locus_tag="PLU99_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18461.1"
                     /translation="MNPAAAKWEALTKPLEGLLGRIRDSERVRGEIGWVVFHWAAEFA
                     LVFAVLKLLTTILGREGYGEYTLVVTATDLVGAVLLSPVIQAFLRTYHPAERDQHERR
                     AGLLVFRWYAAASIGVMTAGALLSKPFAAQFNLHPWSVLVGGLIFTGYQFRMLGVEVL
                     TIRRRRRATALLNCAFLATQILLLLLITSIWGATAQGALTSYALAVLLFAAVIAIPLL
                     CELLGKPDNGAFNLRPLIWSFGVPAGLLLVLQWFQSCGDRYILDRLVDREAVGCYVAA
                     NQVGSAPYFLLYGVLATLLRPIAYQRCNDCNDARGLWAADRLLLGGIGAYVVLGGLAG
                     VGFALCGPELLVLFSSEQFALPRITFVVLAVSRGLQRLGFLLELFFAVHQRMSALLAF
                     RIAGATFTLAICWVMTAGWGLPGAALGSLTAAALNVGLIVLGPGGVLHMIRAARRSLR
                     AQVSEPRGVP"
BASE COUNT          426 a          756 c          936 g          559 t
ORIGIN      
        1 gaactcccac gtgttggatc acgtaggcat cgtggggcgt ttcaaatacg gcgtcgtgct
       61 cctgggcacc gaactctcga gcctgcgcga ctgtagcgtg gtgctgacgg cgccgggcgg
      121 cgtcgggctg tatgccagcc ccgagaaccg tattgacttc tgcagcgcgc gcctacagaa
      181 actgatcgcc ggaggtacca cgacctacaa ccacgtcagc ggcggcgtcc tgggggcggg
      241 ccccggtggc agtagcgtac gatttgatgg ttacgtcgtg gcttggacct tcaccaatgt
      301 cttttttggt gtcgcagccg agccggggtc cgaatcagtg atccgcctgc acggcttgca
      361 atcgccgaac agactgctgg tgccttccca actgacattc atcggctgcg gcaacgatca
      421 gctccggacg cgtttcggac tatatgtgac cagcgagcgt ccggatcgcg tcggggccga
      481 ccgtctgatc ttcgataact gctacctgct ctcgcgcgag tacccggtgt gctgcaagac
      541 cgccgcaaat gaccctggcc cggaaaccgg cctgattcga cattgggccg agcgaaacag
      601 ccgattcctg aaaaacgggg cggtacagga ggtccggccc tagacgagcg agccgagcga
      661 aagcagccgg tttggcacgg agcgtacgaa ggcgatagat ttgcgtgcac gagatggatc
      721 tcgagcaagg cgtgaagatc aggcgcaatg ttccaaagtt ggccgaggcg gcgcgggcgg
      781 ctgcccggcg tggcggtgga gcttggtaag cttccgggca ccgcttcagg atttgcggat
      841 acacggtgcg tccgcctggg cagggccgac cctgcaggcg ccgatgaggc aggcctgttt
      901 gcggcagccg cccacctggg ggtttggcgg ggcctggtcg gcgctggcgc agattatgcg
      961 cgggcttgac agcggggcgg tagtcctggc ggccggcggc aagctgctga cgaagaggcg
     1021 gcggcatgat ttcaaaccgg gaagcttggc gagtgcggtc atggggttga cctacctgag
     1081 tgattacggg tgttcgtcag gcttatcggc gctcgatata cggcccgggc agcggcgctg
     1141 caggtcaaag agcgccggct tgtgttttcg ccaacttgcc tcaagccgcg cggcgccggt
     1201 gcgcgggcgc gcgacggctg ggctgttctc ggttatcgag gcgcccacct tggatgaccg
     1261 accgaccagc gggccgcccg gattttcgag cagatcatga acccggccgc agccaagtgg
     1321 gaagcactaa ccaagcccct tgaggggctg ctggggcgga ttcgcgacag cgaacgagtg
     1381 cgcggcgaaa tcggctgggt ggtgtttcac tgggcggccg agtttgcgct ggtattcgcg
     1441 gtattgaagc tgctaaccac cattttgggg cgggagggct acggcgaata tacgctggtg
     1501 gtgacggcga cggatttggt gggtgcagta ttgctctcac ccgtgattca ggcgttcctg
     1561 cgcacgtacc acccggctga gcgcgaccag cacgaaagac gggccggctt gctggtgttt
     1621 cgctggtatg cagccgcgag catcggggtg atgacggcgg gagcgctgct gagcaagccc
     1681 ttcgccgcgc agttcaactt gcacccctgg tcggtattgg tgggcgggct gatcttcaca
     1741 ggctaccagt tccgaatgct cggggtagag gtgctcacca ttcggcgccg ccggcgcgca
     1801 acagccctgc tcaattgcgc cttcctggcg acgcaaatac tgttgttgtt gctgattacg
     1861 agcatctggg gcgcgaccgc gcaaggggcg ttgacttcgt atgccctagc cgtgctattg
     1921 ttcgcggccg tgattgcgat tccattactg tgcgagctgt tgggtaaacc ggacaacggc
     1981 gcattcaacc tgcggccgct tatatggagc tttggtgttc cggccggtct attgcttgtt
     2041 ctccaatggt ttcagagctg tggcgaccgt tacattctgg accggttggt ggaccgtgag
     2101 gcagtcgggt gctacgttgc cgccaaccag gttggttcag ctccgtattt tctcctgtac
     2161 ggcgtattgg cgacgctact gcgtccgatt gcataccagc gctgcaacga ttgcaatgac
     2221 gcccggggcc tgtgggccgc ggatcgactg ctgttgggcg ggattggggc atatgttgtg
     2281 ttgggcgggt tagccggggt gggctttgcg ctttgtggcc cggaactgct ggtgctgttt
     2341 tccagtgagc agttcgcatt gccgcgcata acgttcgtgg tgctggcagt cagccgcggt
     2401 ttgcagcggc tgggtttctt gctggaactc ttttttgccg tccatcagcg catgagcgcg
     2461 ctactggcgt tccgcatcgc gggcgccacg ttcactctcg cgatttgctg ggtaatgacc
     2521 gcaggctggg gattaccggg cgcggcgctg ggcagtttaa cggcggcggc gctgaacgtg
     2581 gggctgattg tcctcggtcc gggtggagtc ctccacatga tccgcgcggc gcgccggagc
     2641 ctgcgggctc aggtatcaga gccacggggc gtgcctg
//
LOCUS       DAOSYS010000365         2669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_29689_length_2669_cov_4.824024, whole genome shotgun sequence.
ACCESSION   DAOSYS010000365 DAOSYS010000000
VERSION     DAOSYS010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29689_length_2669_cov_4.824024"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLU99_15320"
     CDS             <1..388
                     /locus_tag="PLU99_15320"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOO18462.1"
                     /translation="AAVIPAFVTRMVRGERPVVYGDGEQSRDFCYVDNVVSANLLGLD
                     APRVSGEVVNIACGERVTLNEIIRLINQALGTNITADYQAPRVGDVRHSLADLAAAER
                     VIGYKPLIKFSEGLARSIDWYRQHPA"
     gene            complement(330..1328)
                     /locus_tag="PLU99_15325"
     CDS             complement(330..1328)
                     /locus_tag="PLU99_15325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOO18463.1"
                     /translation="MRSRHVHVSVNLDSIRAAAEAIRLSTGVRLIAVIKADAYGLGAV
                     PISEALATVVDEFAYFTIHEAREVGRPGIILGPPEGDPAEYRELNLRPCVTNRADAER
                     FAGMPVAVKLDTGMQRFGCRVEDLEDLLNRCSAEDVFTHATGMSSVRLLRDVCRCKPQ
                     WLHAASTSLLDCPEAWLDGVRPGLALYRGATRVTSRLAVVRETKGGVGYTRFNCPNVG
                     VILAGYCHNVQKAPVVINGRRQQLLEIGMNTSFVSVDPRDRVGDEVVLLGEELGEAEL
                     AAHFGCREHEILCRYGSMGPRSYVKNGVSAVTVATPALTQDAADTSRSSVPDPRRT"
     gene            1541..2122
                     /locus_tag="PLU99_15330"
     CDS             1541..2122
                     /locus_tag="PLU99_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18464.1"
                     /translation="MIVLAGLLQALSGCAPKAEVVLHQPFAPPSQQELKLASKWAFEA
                     AAGGRRIWLLAFPLPSSPDGPRDFHLYLSTPDVDRGFLGQDFVVDQSSSSGVRGFFIQ
                     EVGQLRGKTQVAAGQVRVSRSWLDWHRRLLEVNLACNDGTKIIGRALADPLASEIAAF
                     ERRYSGDVAALAPPEDTRDLDVDEVPGRATATP"
     gene            2242..>2669
                     /locus_tag="PLU99_15335"
     CDS             2242..>2669
                     /locus_tag="PLU99_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18465.1"
                     /translation="MNNFRIRLTPLVIALLTCWGTLRSDAGCAPAEPAATSVFSPAAY
                     LSHIKYLAGDELGGRGTGSEGGRRAAEYIAEQFQAAGLRPGGVDGTWFQPFEVTVGKR
                     VNDELASLKVEEIERTWRVREDWIPLPFSEFGAVEGPLAFA"
BASE COUNT          471 a          834 c          857 g          507 t
ORIGIN      
        1 cgcggccgtc atacccgcat ttgtgacgcg catggtgcgt ggcgagcggc cggtggtgta
       61 cggggacggc gagcagtcgc gcgatttttg ttacgtggac aacgtggtat ccgcaaacct
      121 gttggggctg gatgcgccgc gggttagcgg cgaagtagtg aacatcgcct gcggcgagcg
      181 cgtcacgctt aacgagatca tccgcctgat caaccaggcc ctgggaacga acatcaccgc
      241 cgattaccag gccccgcgtg taggtgacgt gcgccacagt ctggccgacc tggccgcggc
      301 cgagcgcgtc ataggctaca agccgctgat caagttctcc gagggtctgg cacgctcgat
      361 cgactggtat cggcagcatc ctgcgtaagt gccggtgtcg cgaccgtgac agcactgacg
      421 ccgttcttga cgtagctgcg cggccccatc gacccatatc ggcagaggat ctcgtgctcg
      481 cggcagccga agtgcgccgc cagctccgct tcgcccagct cctccccgag caagacgacc
      541 tcgtcgccca cgcgatcgcg cggatcgacc gatacgaaag acgtattcat gccgatttcg
      601 agcagttgct ggcggcgccc gttgatgact accggcgctt tctgcacgtt gtggcagtac
      661 ccggccagaa tgacgcccac gttcgggcag ttgaaccgcg tataacccac gccgcctttg
      721 gtttcgcgca ccacggccag ccgtgaggtc acccgcgtgg cgcctcgata caacgccagt
      781 cccgggcgca cgccgtccag ccaagcctcg gggcaatcga gcagcgaagt cgaagcggcg
      841 tgcagccact gcggcttgca gcggcaaacg tcgcgcagca ggcgcaccga gctcatgccc
      901 gtggcgtgcg tgaaaacgtc ttccgcgctg cagcggttga gcaggtcttc gagatcctca
      961 acgcggcagc cgaaacgctg catacccgtg tcgagcttta cggcgaccgg cataccggcg
     1021 aaacgctcgg catcggcgcg attggtgacg caggggcgca agttcagctc gcggtactcc
     1081 gccgggtcgc cttcgggtgg tcccagaatg atgcccggtc gtccgacctc gcgtgcttca
     1141 tgaatcgtga agtacgcgaa ctcatcgacc accgtcgcca gggcctccga aatcggcacg
     1201 gcccccagcc cgtatgcgtc ggccttaatg acagcgatca gacgcacacc tgtggacagc
     1261 cgaatcgctt cagcggccgc tcggattgaa tcgagattca cgcttacgtg gacatgtcgg
     1321 cttcgcattt ccctacgcct gacagacttc cttgtccgtt agcgcagtat accaaacgcc
     1381 aattggctta caaggcgatt atgcgcttac cacgcccggc gcaattatcg gaggtctact
     1441 cgtccagttt tcggagccgc taagggcact ggccttcgcg caaaacccca cgtatgctgc
     1501 cgacatgagc atgttcgcct ccgccgctcg aaatactgcc gtgattgtgc tggccggact
     1561 gctgcaggcg ctgtccggct gcgcgccaaa agcagaagtc gtgctgcacc agccgttcgc
     1621 cccgccttcg caacaagagc tgaagctcgc gagtaagtgg gcttttgaag ccgcggcggg
     1681 cggtcggcgg atatggctgc ttgcgtttcc gcttcctagc tctccggatg ggccgcgcga
     1741 tttccatctg tacctgtcga ctcccgacgt ggatcggggc ttcctgggtc aggactttgt
     1801 tgtcgaccag agtagttcga gcggcgttcg cggatttttt attcaggaag tggggcaatt
     1861 gcgagggaaa acgcaggtcg ccgcgggcca ggtgcgggtg tcgcggtcct ggcttgactg
     1921 gcacagacgt ctgctggaag tgaatttagc ctgcaacgat ggaaccaaga ttatcggacg
     1981 ggcgctggca gatccattgg cgtccgaaat cgcggccttc gaacgtcgct actcgggcga
     2041 tgtcgccgcc ctggccccgc cggaggatac gcgagacctg gatgtcgacg aggtgcccgg
     2101 ccgggctacg gcgacgccct gatttccaaa cctgaagcac acggggcccg ccaagcaacc
     2161 gggccgtatt tgttcgttgc gctcgtgcgg gctaaactga ctacgagcga cctgtgaccc
     2221 cctgggccgt ggtaaatccc gatgaacaat tttcgaattc gattgacgcc gctggttatc
     2281 gcgctgttga cctgctgggg cacgctgcgg tctgacgccg ggtgtgcgcc ggccgagccc
     2341 gcagctactt ccgttttttc gcctgctgcc tatttgagcc atatcaagta tttggccggg
     2401 gacgagctag gcggtcgcgg aaccggcagc gaaggcggcc ggcgggcggc agagtacatc
     2461 gccgagcagt tccaggcggc cggcctgcga cccggcggcg tggacggcac gtggttccag
     2521 cccttcgagg tgacggtagg caagcgcgtc aatgacgagt tggcgagcct gaaggtggaa
     2581 gagatcgagc gcacctggcg tgtgcgggag gactggattc cgcttccatt ctccgagttc
     2641 ggcgcagtgg aaggaccgct ggcttttgc
//
LOCUS       DAOSYS010000366         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_30006_length_2648_cov_6.165060, whole genome shotgun sequence.
ACCESSION   DAOSYS010000366 DAOSYS010000000
VERSION     DAOSYS010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30006_length_2648_cov_6.165060"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..974
                     /locus_tag="PLU99_15340"
     CDS             189..974
                     /locus_tag="PLU99_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOO18466.1"
                     /translation="MDARDPIIELRQVSKSFGRRIVLDCIDLKIQRGTSTVIIGRSGT
                     GKSVLLRHIVGLHRPDHGEVYFEGRRIDRLSERQLVPIRRQISFVFQLNALFDSMSVV
                     ENVAFPMTETGNGRADSRQIAALARRSLQMVGMAGFEDKRPADLSGGEKKRVALARAI
                     AMDPPPKVILYDEPTAGLDPQRGDVITRLIRQLQREVNVTSVVVTHDMKLAAQAGDRV
                     LLLWNGRFIGDGTPEELLSSRDEHVRRFVDGLAEPEDLATLSQ"
     gene            complement(1070..>2648)
                     /locus_tag="PLU99_15345"
     CDS             complement(1070..>2648)
                     /locus_tag="PLU99_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18467.1"
                     /translation="QPFVVLLTGQEPVAAQAFDSLDGIRECSFAPTPNADGLRAQLVS
                     LSASGPETIAETEVAADPSRALQIDLTPGERLLPGQRIALPFEVRSAAGRHDDPPAAS
                     NTASASLLALLTPALWSGPVEWAPGSPSVDSNTSTGSGAPMAASWGSAATEQRPATPQ
                     RPNVPPPLAVGGTLWATSRAAPDKTLELTLPQTPGVYRLAALARDEQGTLTSKDILLD
                     TRAGLRLLPDVPARCTLGDRFVIGMAVQNPHAVPVTVNLTVDPGAGLSIESLRVIDPP
                     ENSNVAPVEPLAREVVLPASGVAWLQARVEAVQSGQSDLKMSTVVRAVQPQAGVPDSA
                     SNGPVRPQPGDAQDISVPYAVSADGQSDNAGRPLRISRTLLLLEKDDEPPDAESEDLG
                     PRRTSPSRWHRYVLPPGARLKPGQRILVRESFELAQPATAMKWSQRIPPNCSLVAQPQ
                     SEPRALGLLEPHRADQLDFRARGITAGPHEHEYELVATRPGVCVLPMPEIWISGQRVD
                     TAGEPEELRLHVLPAAE"
BASE COUNT          453 a          817 c          886 g          492 t
ORIGIN      
        1 ccgaaggcgt cggccgcgcc tgtaccgagg cttttgtctc cagtttcatc gtgattctga
       61 tgctggattt cttcctgaac ttcgttaaca acggcgtgta caaagcttta tacggtacgc
      121 gctcgctgtt ttgagccgga gagccggata ggggaaaccg tggagtcatt taaccgcgag
      181 accgataagt ggacgctcgg gacccgatta ttgaactgag acaggtcagc aagtccttcg
      241 gacggcgaat cgttctggac tgcatcgacc tgaaaatcca gcgtggaacc tcgactgtta
      301 ttatcggtcg ttcaggaacc ggcaagagcg tcctgctgcg ccacatcgtc ggcctgcacc
      361 ggcccgatca tggcgaggtg tattttgagg ggcggcgaat cgaccggctc tcggagcgac
      421 aactcgttcc gatccgccgg cagatcagct tcgtgtttca gctcaacgcg ctcttcgact
      481 cgatgagcgt cgtggagaac gtcgcctttc caatgaccga aaccggtaac ggccgggctg
      541 attcacgcca gatcgccgcc ctggcgcggc gctctctcca gatggtcggg atggccggtt
      601 ttgaagacaa gcggccggcg gatttgtcgg gcggtgagaa gaaacgcgtc gccctggcgc
      661 gcgctatcgc gatggacccg ccgccgaagg tcattcttta cgatgagccg acggccggtc
      721 tcgatccgca gcgtggcgac gtcatcacgc gtctcattcg ccagctccag cgcgaagtca
      781 acgtcaccag cgtagttgtc acgcacgata tgaagctggc ggcccaggcc ggcgaccgcg
      841 tgctgctgct ttggaacggg cgcttcatcg gcgacggcac gcccgaggag cttctcagca
      901 gccgcgacga acacgtgcgc cgcttcgtcg acggtttggc ggagccggag gacttggcga
      961 cgctgtcgca gtgacgctat cgccgtgaca ccatcgccgc ggccggctgg gaataagaag
     1021 cttctctact tcgccgtacg aagcacgcta cttcgccgta ggaagcacgc tactccgctg
     1081 cagggagcac gtgcagccgc agctcctctg gttcgcccgc tgtgtccacg cgctgccctg
     1141 aaatccagat ttcgggcata ggcagaacgc acacgcccgg ccgcgtggca actaactcgt
     1201 actcatgctc gtgcggtccg gcggtgatgc cacgagcgcg gaagtctaat tggtccgcgc
     1261 ggtgcggttc gagaaggccc agtgctcgtg gctcggattg cggttgggcg acgagtgagc
     1321 aattcggcgg gatgcgctgg ctccacttca ttgcggttgc gggctgcgcc agttcgaaag
     1381 actcgcggac gagaatgcgc tgccccggct tcaggcgggc gcccggaggt aggacgtagc
     1441 ggtgccagcg agatggtgac gtccggcgcg ggcccaaatc ctcggattcg gcgtcgggag
     1501 gctcgtcgtc tttctccaga agcagcagtg tgcggctgat acgcagcggg cggccggcat
     1561 tgtctgattg gccgtcggcc gagactgcat aggggacgct gatatcctga gcgtcgcccg
     1621 gttgcggtcg tacgggcccg ttggaagcgg agtcaggaac gccggcttga ggctggacgg
     1681 cccggacgac ggtcgacatt ttcaaatccg actggccgga ctgcaccgct tcgacgcgcg
     1741 cctggagcca ggccacgccg ctggccggca ggactacctc gcgcgccagc ggctcaaccg
     1801 gtgcgacgtt ggagttttcg ggcgggtcga tgacccgcag ggactcgatg ctcagtcctg
     1861 ctccgggatc gaccgtcaag tttacggtga caggtactgc gtgcgggttc tgcaccgcca
     1921 tgcctatcac gaaccgatca cctaacgtgc aacgtgccgg cacatccggg agaagtcgca
     1981 agccggcccg tgtgtcgagc agaatatcct tgcttgtgag tgttccctgc tcgtcgcgcg
     2041 ccagggccgc aagccggtat acgccgggag tttgcggcaa agttagctcc agggtcttgt
     2101 ctggcgcggc ccgggatgtg gcccagagcg tgccgcccac ggccaacggc ggcggaacgt
     2161 tcggacgctg aggagtcgca ggccgttgtt ccgtcgccgc gctgccccac gaagcggcca
     2221 tcggcgcccc cgagcctgtc gaggtgttcg agtcgactga gggcgagccg ggcgcccatt
     2281 caaccgggcc ggaccacagg gcaggcgtga gcagcgctag caagctcgcg gaagccgtat
     2341 tgcttgcggc tggcgggtca tcatggcgac ccgccgcgga tcgcacctca aacggcaggg
     2401 cgatgcgctg acccggcagc agtcgttctc ctggcgtgag gtcgatttgc agggcgcgtg
     2461 acgggtctgc cgcaacttca gtctcggcaa tagtctcagg tccgctcgcg ctcaggctta
     2521 ccaactgtgc gcgcagtccg tcggcattcg gcgttggtgc gaatgagcac tcgcgaatcc
     2581 catcgaggct gtcgaaggcc tgcgcggcga ccggttcctg gccggtgagc aacactacga
     2641 acggctgc
//
LOCUS       DAOSYS010000367         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_30421_length_2619_cov_5.843214, whole genome shotgun sequence.
ACCESSION   DAOSYS010000367 DAOSYS010000000
VERSION     DAOSYS010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30421_length_2619_cov_5.843214"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..51
                     /locus_tag="PLU99_15350"
     rRNA            <1..51
                     /locus_tag="PLU99_15350"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            235..894
                     /locus_tag="PLU99_15355"
     CDS             235..894
                     /locus_tag="PLU99_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18468.1"
                     /translation="MNWKALLQETLSGLARTHAHEPLSEIQRILRGVLGLDEIQFQGP
                     DGHELRADTTLQQALEQYAAVAVKLQIKPVAAPAAAPSAAAPAAESRFREGAPAADVS
                     KLSMDERTDMFLREFMRLENRHDFMWAGYIVRELLPRLGFAPEEAKLVLDHLRAEKLV
                     TISKVPNPKNPDFPATGVHLNREHPRVKALLASHPRNDGATAAPPVRESREAPAASPQ
                     P"
     gene            1010..1570
                     /locus_tag="PLU99_15360"
     CDS             1010..1570
                     /locus_tag="PLU99_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOO18469.1"
                     /translation="MKAARQPSHEASRAEADDLRSRVCYDDARLLAECEIHTFRASGP
                     GGQKRNKTSSAVRLHHAPSGIIVKAAESRSQHENKARALRRLREALAIGFRRPLPNAV
                     TWPEHVHVVDGTLRLASNNPDLAEVLGLLLDALDAARGRTNDAAACLGISAASLTRVL
                     SEHHAAWAEANRIRQAASLPALRTPS"
     gene            1744..2388
                     /locus_tag="PLU99_15365"
     CDS             1744..2388
                     /locus_tag="PLU99_15365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOO18470.1"
                     /translation="MERFEIGPSPDDPFPLPGQPRLAFLKNHITRPNIIVGDYTYYDD
                     PDGVANFEKNVLYHFDFIGDKLIIGKFCAVGTDTKFIMNGGNHAIEGFSTYPFRIFGG
                     AWERNLPADFAFPHRGDTVVGNDVWLGYGGLIMPGVKIGDGAIVASRAVVTKDVPPYA
                     IVGGNPARVIRMRFDEATVAALLEIRWWDWDIEQVTRNLAAITGTDIERLRACT"
BASE COUNT          492 a          842 c          834 g          451 t
ORIGIN      
        1 aagtcgtaac aaggtagccg taggggaacc tgcggctgga tcacctcctt tctagaggat
       61 acctagatcg ctccggcggc ttgccgcctg agcgaactaa aatcagcgca ttcttacctg
      121 gcccttagcg gctggggcgc ggcggagaaa tccgtcgccc ggcaacgtcc actgttctct
      181 ttttttatgc gccctattgt cggcgctttt ggcccaaatt aagggcaacc aaagatgaac
      241 tggaaagcgc tcctccagga aactctttcc ggcctggcgc gaactcacgc gcacgaaccc
      301 ctctccgaaa tccagcgcat tcttcgtgga gtactgggcc tggacgaaat ccaattccag
      361 gggcccgacg gccatgagct gcgcgccgac accacgctcc agcaggcgct cgaacagtac
      421 gctgcggtgg cggtcaagct acaaataaag ccggttgcgg cgcccgccgc tgcgcccagc
      481 gctgcggccc ccgctgccga atcgcgcttc cgcgagggcg cgccggccgc ggacgtttcg
      541 aaactttcga tggacgaacg cacggatatg ttcctgcggg agttcatgcg cttggaaaac
      601 cggcacgatt tcatgtgggc cgggtacatc gtgcgcgagc tactgccgcg gctgggcttc
      661 gcgccggaag aggctaagct ggtgctggat catctgcggg cggagaagct ggtcacgatc
      721 agcaaggttc ccaatcccaa gaacccggat ttcccggcga ccggcgtgca cctgaaccgc
      781 gagcacccgc gcgtcaaagc cctgctggcc agccacccgc gcaacgatgg cgcgaccgcc
      841 gctccgccag tacgtgaatc gcgtgaggcg cccgcggcga gcccacagcc gtagttgcgc
      901 cggcccgggt cgcgccgcca cggcagtgac cgacgggggg catgcccgcg cttacagctt
      961 ggggcatggc acccaacatt gtatccgtat actcgcgtag atggcagaga tgaaggcagc
     1021 gaggcaacca agccacgagg cgagcagggc ggaggccgac gacctgcgct cgcgcgtgtg
     1081 ctatgacgat gcgcgcttgc tagctgaatg cgaaattcac accttccgtg cgagtggacc
     1141 gggcggacag aaacgcaaca agacctcgtc ggccgtccgg ctgcaccatg cgccgagcgg
     1201 gatcatcgtc aaagcggccg agagccggtc acagcatgag aacaaggccc gggctttgcg
     1261 gcgcctgcgg gaagcgctgg caatcggttt ccggcggccg ctgccaaacg cagtgacctg
     1321 gccggagcac gtgcacgttg ttgacgggac gttgcggctg gcgtccaaca atccggattt
     1381 ggcggaggtg ctcgggctgt tgttggacgc tttagacgca gcgcgcgggc gaacgaatga
     1441 cgcggccgca tgcctgggta tcagcgccgc cagtctgacc cgcgttctgt cggagcacca
     1501 cgcggcctgg gcggaggcga accggattcg gcaagcggca agtttgccgg ccctgcggac
     1561 gccgagttaa acggccggcc ggcactacct gtgacggcgc tacctgtgta tggaggtgtt
     1621 gaaacgcgag caatgctcac cgagcagtcc accttcccac ctttccacgt taccaccttc
     1681 ccactcttct ggagtcatcg atgaatctct ctaaatttaa ggaagagaag aggagcgaga
     1741 gacatggagc ggtttgaaat tggcccttcg ccggacgacc cgtttccgct gccgggtcag
     1801 ccgcgcttgg cgtttctcaa gaaccacatc acgcggccga acatcatcgt cggcgattac
     1861 acctattacg atgacccgga cggcgtcgcg aattttgaaa agaacgtcct gtaccacttc
     1921 gacttcatcg gcgacaagct gatcatcggg aaattctgcg cggtgggtac cgacaccaag
     1981 ttcatcatga acggcggcaa tcacgccata gagggcttct caacctaccc gttccgcatt
     2041 ttcggcggcg cgtgggagcg caatctgccg gcggacttcg cgttcccgca ccgcggcgac
     2101 acggtcgtcg gcaacgatgt ctggctcggc tatggcggcc tgatcatgcc gggcgtgaag
     2161 atcggcgacg gggcgatcgt cgcctcccgc gccgtggtta ccaaggacgt gccgccttac
     2221 gccatcgtcg gcgggaatcc ggcccgcgtg atccgcatgc ggtttgatga ggcgaccgtg
     2281 gcggccctgc tcgaaattcg ctggtgggac tgggacatcg aacaggtcac gcgcaacctg
     2341 gccgcgatca ccggcacaga tattgaacgg ctgcgggcct gcacgtagcg gccggcctgc
     2401 cttatcgcga tcgcggcctg tttggctgcg ctttccgacg cctcaggtct gcagcgccgg
     2461 cggggcgccg ggcgcagcct cgggcgcgcc gccccggccc aggctgtgcg cggtcaaacc
     2521 gagtgcgatc cacgacacgc cttggacgat gagttcaacg cccacacata gcccgatgaa
     2581 ccacaggccc gtcgcgggcc agttctgcca gactgccag
//
LOCUS       DAOSYS010000368         2619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_30422_length_2619_cov_5.741810, whole genome shotgun sequence.
ACCESSION   DAOSYS010000368 DAOSYS010000000
VERSION     DAOSYS010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30422_length_2619_cov_5.741810"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLU99_15370"
     CDS             <1..396
                     /locus_tag="PLU99_15370"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOO18471.1"
                     /translation="LCVGETLLEREAEQTPDVLTFQLTAALVGVDLRAARDLVVAYEP
                     VWAIGTGRNATPEQAQEAHAHLRQQLRRIAGPIAEDIRILYGGSMKPENARDILAQPD
                     VDGGLIGGASLKADSFVGIVNAALATQGE"
     gene            478..1065
                     /gene="secG"
                     /locus_tag="PLU99_15375"
     CDS             478..1065
                     /gene="secG"
                     /locus_tag="PLU99_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOO18472.1"
                     /translation="MILAAAHHVILAFFFALVGILLIGVILLQRGKGVGLAGAFGGTG
                     GHTAFGAKTGDFLMWVTVAGAGVLLAFTIILNFVFVPTTVTLQAAPMVPPPMAPVPGA
                     PGPMPGGPPMSPAAPAPALPPAAEMPMPAAPSPDAPAPEPAPAPEPAAESETPAPAPE
                     TPAPGGGTPADEENPIPGPVFGAWRAPLAVFGEIA"
     gene            1089..1967
                     /locus_tag="PLU99_15380"
     CDS             1089..1967
                     /locus_tag="PLU99_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOO18473.1"
                     /translation="MTGFGEAQLEADGHSYHVEIRSVNNRYFKAALHLPEGFAFLEPE
                     VERLLRARLSRGSVSLRIRVRDLTESAAADLNAAALREYVSQLTAIAATAGGAQQVII
                     DLAALAALPGVAQPHELTDAERERAWAIIKELIQQALERLVQMRTVEGKALAAELQQH
                     CERIRRHITVIRGRADTVVREYRDRLMTRIRELIADSNVRLAEEDLLKEVAIYAERSD
                     ICEELSRLDVHLAQFEQMSAGPEAAGRKLEFVAQEMLREANTIGSKAGDTHIARDIID
                     IKSAIDRIKEQVQNVE"
     gene            complement(2001..2588)
                     /locus_tag="PLU99_15385"
     CDS             complement(2001..2588)
                     /locus_tag="PLU99_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOO18474.1"
                     /translation="MWEIRSARQHHVHSKVMCWQCVDRALKLGEYLGRRKRDWEALRD
                     EIAADVLQHGYHAEERRILAWYADHAPDAAALQVGLSGLLPPDDERFRNTVEYVERHL
                     RVGPTVYRYSYDDGLPGMEGGFNLCTTWLIEAYALLGRHKDAQELFERYTKLVGSTGL
                     LSEEYDPGAQQSLGNYPQAYSHLGLINAALVLDRA"
BASE COUNT          462 a          884 c          829 g          444 t
ORIGIN      
        1 ctctgcgtcg gcgaaaccct gctggagcgc gaggccgagc agacccccga cgtcctcacc
       61 ttccagctca ccgcggccct ggtcggcgtc gatctgcgcg cggcccgcga cctggtcgtc
      121 gcctacgaac cggtctgggc catcggcacc gggcgcaacg ccacccccga gcaggcccag
      181 gaggcccatg cccacttgcg ccagcaactg cggcgcatcg ccgggccgat cgccgaggac
      241 attcgcatcc tctacggcgg cagcatgaaa ccggaaaacg cccgcgacat cctggcccag
      301 ccggacgtcg acggcggact catcggcgga gcgagcctga aggcggattc ttttgtggga
      361 atcgtcaacg cggctctggc aacccagggc gaatgacgct atagttaacg attctcacgg
      421 tcggcggcgc cgccggccgt cttacgccac gcgaatggca ttgaggttga gataggaatg
      481 atcctggccg ctgcacacca cgtgatcctc gcgttcttct tcgccctcgt ggggatattg
      541 ttgatcggcg tcattctcct gcaacgcgga aagggtgtcg gtttggccgg cgctttcggc
      601 gggaccggcg gacataccgc gttcggggcc aagaccggcg atttcctgat gtgggtgact
      661 gtggccgggg ccggagtcct gctggcgttc acgattatcc tgaacttcgt cttcgtaccg
      721 acgacggtca ccttgcaggc cgcaccaatg gttccgccgc ccatggcccc cgtgccgggt
      781 gccccgggcc ccatgcccgg tggaccacct atgagcccgg ccgcgcctgc gccagccctg
      841 ccgcccgctg ccgaaatgcc gatgccggcc gcaccgtcgc cggacgctcc ggctcccgag
      901 ccggctccag cacctgagcc ggccgctgaa tccgagactc cggctcctgc gcccgaaact
      961 ccagcgccgg gcggcgggac gcctgcggac gaggaaaacc cgattcccgg accggtattc
     1021 ggcgcgtggc gcgctccgct agcggtgttc ggggaaattg cctgaccggg tagtccatga
     1081 tccagtcaat gaccggcttc ggcgaggcgc agctcgaagc ggacggtcat tcctaccacg
     1141 tcgaaatccg cagcgttaat aaccgctatt tcaaagctgc tctgcacctg cccgagggct
     1201 tcgccttcct tgagcccgag gtggagcgcc tgctcagggc gcgcctcagc cgcggcagcg
     1261 tcagcttacg catccgcgtc cgcgatctga cggagtccgc ggcggcggat ttgaatgcgg
     1321 ccgcgttgcg cgagtacgtc tcgcagctca ccgcaatcgc cgctacggcc ggcggcgctc
     1381 aacaagtcat cattgacttg gcggccctgg cggctttgcc gggcgtggct cagccgcacg
     1441 agttgaccga tgccgaacgt gagcgcgcct gggcgattat caaggagctg atccagcagg
     1501 ctctggagcg cctggtgcag atgcgcaccg tggaggggaa ggccctggcc gcggaactgc
     1561 aacagcactg cgagcgcatc cgccgccata tcacggtcat tcgcgggcgg gccgacacgg
     1621 tggtccgcga gtatcgcgac cggctgatga cccgcatccg cgagctgatc gccgacagca
     1681 acgtccggct ggccgaagag gacctgctga aggaagtggc catttacgcc gaacggagcg
     1741 atatctgcga ggaactcagc cggctggacg tccacctggc gcagttcgag cagatgtccg
     1801 ccgggccgga agcggccggc cgcaagctgg agttcgtggc ccaggagatg ctgcgtgaag
     1861 ccaacacgat tggctcaaag gcgggcgaca cgcacattgc gcgcgacatc atcgacatca
     1921 aaagcgcaat cgatcgcatc aaagaacagg ttcagaacgt cgaatagact cgcgcggggg
     1981 ctctggtcaa tcaatctttg ctaggcccgg tccagcacga gtgcggcgtt gatcagcccc
     2041 agatgcgaat aagcctgcgg ataattcccg agcgattgct gcgccccggg atcgtactct
     2101 tccgacagca gccccgtaga gccaacgagc ttcgtgtagc gctcgaacag ttcctgcgca
     2161 tccttatgcc ggcctagcag ggcataagcc tcgatcagcc aggtcgtaca cagattgaag
     2221 ccgccctcca tgcccggcag accgtcgtcg tagctatagc ggtagacggt cggaccaacg
     2281 cgcaggtgcc gctccacgta ttcaacagta ttgcgaaacc gctcatcgtc gggcggcagc
     2341 agcccactta gcccgacttg cagggcggct gcatccggcg cgtggtcggc ataccaggcc
     2401 aggatgcgcc gctcttcggc atggtagccg tgttgcaaca cgtcggcagc gatttcgtcg
     2461 cgcaaagcct cccagtccct tttgcggcgt ccgagatatt cgcccaactt cagtgcgcgg
     2521 tcgacgcact gccagcacat taccttggag tgaacgtggt gctggcgcgc actgcgtatt
     2581 tcccaaatgc cgtggtccgc ctaacgccag cgctgcccc
//
LOCUS       DAOSYS010000369         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_31115_length_2572_cov_4.083433, whole genome shotgun sequence.
ACCESSION   DAOSYS010000369 DAOSYS010000000
VERSION     DAOSYS010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31115_length_2572_cov_4.083433"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLU99_15390"
     CDS             <1..623
                     /locus_tag="PLU99_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOO18475.1"
                     /translation="EAGRVVPPEFGGIKMISMGHLVSPDQAVIWRGPMAQKYVKEFLD
                     RGAWGELDYLLVDLPPGTGDIPLTLAQSIPLTGAVVVCTPQDVAVLDVLKAVRMYQKL
                     GVEPLGMVENMSYYVCPHCAARDYPFGQGGAERAAAEIGVPFLGGIPLNIAIREFGDA
                     GRPFAHFTDAGQEVVRALEQVVTNLVERVSNLRRARTPLPQLKVQS"
     gene            935..1969
                     /locus_tag="PLU99_15395"
     CDS             935..1969
                     /locus_tag="PLU99_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18476.1"
                     /translation="MPRPDFKHRDAVYQTLCAQIVREATDLQSLAASAARLLGHPDVS
                     SDPMLGAMLRGFIAEREAYLRQARVLAHPEQAQDKSQLKKPFEQLGSTEFPTKARVLK
                     ALLSSRHEFEVSLAEYNEADARRALQNIEDLGRRFPVHVEAAVVRDCRQKFEAFVARC
                     ERYRQQVEQVAAQAVEAARRGEPKTADWLLRRLRAIHALTPVLLSAERFEAIAQQIQR
                     VSQKHAQREARAALIARERAVADRIKRAGAAIYRFHKASAELPPESEEYQRAEAAYNA
                     AVEEVRSLDTDWLTGLLLDLETYLDDLHDPEGRTEMQLDRFIGTVRVALRQLRQEIRA
                     ITAARQREAP"
BASE COUNT          460 a          901 c          799 g          412 t
ORIGIN      
        1 acgaagccgg ccgcgtcgtg ccgccggagt tcggcggcat caagatgatc agcatggggc
       61 acctggtttc gccggatcag gcagtgatct ggcgcggccc gatggcccaa aaatacgtca
      121 aggagtttct tgatcgcggc gcgtggggcg agttggacta tctgctcgtc gacctgccgc
      181 cgggcacggg cgacattccg ctgacactgg cgcaaagcat tccccttacc ggcgccgtag
      241 tagtctgcac cccgcaggac gtggcagtgc tggacgtgct gaaggcggtc cgcatgtacc
      301 agaagctcgg cgtcgagccg ctcggcatgg tcgagaatat gagctattac gtctgcccgc
      361 actgtgcggc ccgcgattat cccttcggcc agggcggcgc cgagcgcgcc gcagccgaga
      421 ttggcgttcc gttcctgggc ggcattccgc tgaatatcgc gatccgcgag ttcggcgacg
      481 ccggccggcc gttcgcgcat ttcaccgacg ccggccagga ggtcgtgcgg gcattggaac
      541 aggtcgtcac caacttggtc gaacgcgtca gcaacctgcg ccgcgcccga acccccctgc
      601 cgcaactcaa ggtccaaagc tgacttgtcg caattcggcc gccatcggcc ggcggcgcgg
      661 ctagactaat agcggataga catactggag ccgcgcgacc ggacggtttt cactcgcggc
      721 ggttctcact tgtgcactgc cgtctcgcca acggctgatt gtcttggtac tgccgttcgc
      781 tgccggccga cgctcgtgac tccgctgtcc actcgcgact ccgccgtctc ggcctgatcg
      841 acatttgcgg cgcctttgtc ggcgtggctg acacttgtac cgctgtctcg gccgtggcgg
      901 ctcgcctttg cactgcggtt tggagcatcg ggtcatgcct cgaccagact tcaagcaccg
      961 cgacgcagtt taccagactc tgtgtgccca gatcgtgcgc gaagcgaccg acttgcagag
     1021 tctggccgcc tccgccgccc gcctcctggg gcacccggac gtgtccagtg accccatgct
     1081 gggcgcgatg ctgcgcggct tcattgcgga acgcgaggcc tatctgcgcc aggccagggt
     1141 cctggcgcac cccgagcagg cccaggacaa gtcgcagctc aaaaagcctt tcgaacagct
     1201 cggcagcacg gagttcccga ccaaggcgcg ggtgcttaag gcgctgctca gctcgcggca
     1261 tgaattcgag gtctcgctgg ccgagtacaa cgaggccgac gcgcgccgcg ccctacaaaa
     1321 tatagaggac ctgggacgcc gcttcccggt gcacgtcgag gccgcggtgg tccgggactg
     1381 ccggcagaag ttcgaggcgt tcgtagcgcg ctgtgagcgg taccgccagc aggtcgaaca
     1441 ggtggccgcc caggcggtcg aggcggccag gcgcggcgag ccgaagaccg cggactggct
     1501 actgcggcgc ctgcgggcca ttcacgccct gacgccggtg ctgctgtcgg ccgagcgctt
     1561 tgaggcgatt gcccagcaaa ttcagcgcgt gagccagaag cacgcccagc gcgaggcccg
     1621 cgccgcgctg atcgcgcgcg agcgggccgt ggccgaccgc atcaagcgtg ccggggccgc
     1681 catctaccgc ttccataagg ccagcgccga gttgccgccg gagagcgagg agtaccagcg
     1741 ggccgaagcg gcctacaacg ccgcagtcga agaagtgcgg agtctggaca cagactggct
     1801 gacggggcta ttactggact tggagactta cctggacgac ctgcacgacc ccgaaggccg
     1861 caccgaaatg caactggacc gcttcatcgg aacagtgcgc gtagccctaa gacaactccg
     1921 ccaagaaatc cgcgcaataa cggccgcacg ccaacgcgaa gccccctagc cccacagcag
     1981 agccggggcg tgtcgccccg gaataccgcc aagaattccc ccatccgcac agtcgccgcc
     2041 ccgcctccgg cgtgtcgccc cggaatgccg ccgctaatct gtcatccgcc gctcgtgcgg
     2101 gtgccatgcc caagcccgcg ttgcgggcgc gggcatgccc cggcagtaac cgcgcagagc
     2161 agagtcggcg cgtgccgccc cggaatgatg ccaacgtgtc gcaactcgcc tttatgcgag
     2221 cagagccggg gcgtgtcgcc ccggaatgcc gccaacaagc tcgcatccgc gcagtcgtcc
     2281 gccccacgac ccgagcccgc ctaatccgag cccgaagcgc cagcgagggc acgtagcgtc
     2341 ctccccgaaa ggtagcgtcg gcccgccgcg accacattcc cacgttccca cattcccacg
     2401 ttcccacgtc cggcaagact aaggactaac aactaacaac tcttcgccct acattcccac
     2461 attcccacat tcccacgttc ccactttccc acgttttcac gcgtgcgtgc ccatcgttca
     2521 gggccaccgg ctgatgcagc gcagaaagcc tgaaaccaac tgcaataaaa aa
//
LOCUS       DAOSYS010000370         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_32201_length_2503_cov_5.229575, whole genome shotgun sequence.
ACCESSION   DAOSYS010000370 DAOSYS010000000
VERSION     DAOSYS010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32201_length_2503_cov_5.229575"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLU99_15400"
     CDS             complement(<1..226)
                     /locus_tag="PLU99_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18477.1"
                     /translation="MKNVLIILGVLVLVVGVAFYKSTQTESPAVVPDDNPVVHTQSSQ
                     SLAQPVALPETASQPPPSVPLSEKGTPCPEP"
     gene            complement(226..864)
                     /locus_tag="PLU99_15405"
     CDS             complement(226..864)
                     /locus_tag="PLU99_15405"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOO18478.1"
                     /translation="MHPVTYPRILRFTATLIGVPLLGLLCANAGLLYGCGRSHGVQAD
                     SQAVNPMQPAPLTPAPEAQPGPPVSQARPAEAPVPPAAAPEELQAAPVANPAAGKFVA
                     YYFHRTMRCKTCLAIEGNSKEAIEEGFAADLKSGSLAWRTVNIEEPANQHFESDFNLE
                     SSSLVLVEMNGDQVGRWQILESVWDLVDQPPALKQHVRTELAKFMAGGKAAP"
     gene            complement(917..1162)
                     /locus_tag="PLU99_15410"
     CDS             complement(917..1162)
                     /locus_tag="PLU99_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOO18479.1"
                     /translation="MKIEILGTGCKKCETLAANAKAAADKLGLAYELVKVTDLSKIAQ
                     FGVLVTPALAIDGEVKFAGKVPSEAELTSLLTTAAQS"
     gene            complement(1192..2361)
                     /locus_tag="PLU99_15415"
     CDS             complement(1192..2361)
                     /locus_tag="PLU99_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008317631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOO18480.1"
                     /translation="MIAVFLLSYFLPFKSIPAGQTRIANAGREALLMLQDYAQHHVIL
                     CLVPALFIAGAISCFVSQVSVIKYLGPTANKFLAYTVASVAGTILAVCSCTVLPLFAG
                     IWTRGAGLGPASAFLYSGPAINVLAIILTARILGWQIGAARAGAAIAFSFIIGLAMHF
                     IYRKEERAKAEEAKAAGGFVLPDDAGQRPLWKTAAYFLVMVGILVFANWARPATANAA
                     WEAIFAAKWAITAVLVVALAVMLKFWFTPAELKQWVGSTWFFTKQILPLLFFGVLVSG
                     VLLGRPGQEALVPNRWIEQAVGGNSLWANFFASFAGVLMYFATLTEVPILQGLIGSGM
                     GQGPALALLLAGPALSLPSMLVIRSVVGTQKMLVYVALVVALSTVAGLLYGICAG"
BASE COUNT          470 a          774 c          817 g          442 t
ORIGIN      
        1 atggctcagg gcagggggtt cccttctcgg agaggggaac gctcggtggc ggctgcgagg
       61 cagtctcggg cagcgcaacg ggctgagcaa gggactgcga actttgtgta tgaacgacgg
      121 ggttgtcgtc cggcacgacc gccgggcttt cggtctgtgt ggacttgtag aacgccacgc
      181 cgacgacgag cacgagcacg ccgagaatga tcagcacgtt cttcattacg gagctgcctt
      241 tccgccggcc atgaacttgg ccagttcggt tcgcacgtgc tgctttagcg ccggtggctg
      301 gtcgaccagg tcccagacgc tttccaaaat ctgccagcgc ccaacttggt cgccgttcat
      361 ttcgacgaga accagcgatg aggattcgag attgaagtcg ctttcgaagt gctggttcgc
      421 cggctcttca atattgaccg tccgccacgc caagctgccg ctcttcaggt cggccgcgaa
      481 gccctcttca atggcctcct tcgaattgcc ttcgatcgcc aggcaggtct tgcagcgcat
      541 ggtgcggtga aagtaatagg cgacgaactt gccggcggca gggttggcga ccggcgcggc
      601 ctgaagctcc tcgggggcgg ccgcgggcgg cacgggtgct tccgccgggc gtgcctggct
      661 gactggcggg cccggctgcg cctcaggcgc gggggtgaga ggcgcgggct gcatgggatt
      721 tactgcctgc gagtccgcct ggacgccatg cgatcggccg cagccgtaaa gcagtccggc
      781 atttgcgcac agcagcccca gcagcgggac tccgatcagt gtggccgtga accgcaagat
      841 acgcgggtaa gttactggat gcatgaaaga ctcctcagca gcgcccgcag ggggcgtgaa
      901 ccgtccgaca gggccgtcag ctttgagcgg cagtagtcag caagctcgtc agttccgcct
      961 cgctcggcac ctttccggcg aactttactt cgccgtcgat agcgagcgcc ggcgtgacga
     1021 gaacgccgaa ttgcgcgatt ttcgacaaat ccgtgacttt gactaattcg tacgccaggc
     1081 cgagtttgtc cgcggcggcc ttggcgttgg cggccagggt ttcacatttc ttgcagccgg
     1141 tgccgaggat ttcgattttc atgtcgcttc cttttttaca cttttggatt gctaaccggc
     1201 gcaaatgccg tacagcagtc ctgccacggt tgagagcgcc accaccagcg ctacgtaaac
     1261 cagcatcttc tgggtgccga cgaccgaccg gatgaccagc atgctcggca atgagagcgc
     1321 ggggcccgcc agcagaaggg ccagcgccgg accctgcccc atgcccgagc cgatcaggcc
     1381 ctgcaaaatc ggtacttcgg tcagggtagc gaagtacatc aggacgccgg cgaaactggc
     1441 gaagaagttc gcccataggc tgttcccgcc gaccgcctgc tcgatccagc ggttcgggac
     1501 aagcgcttcc tgccccggtc gccccagcag cacgccggac acgagtaccc cgaagaacag
     1561 caacggcaga atctgtttcg tgaagaacca cgtgctgccg acccactgct tcagctccgc
     1621 gggcgtgaac cagaacttga gcatgacagc cagcgccacc accagcacgg cggtgatcgc
     1681 ccatttggcg gcgaagatcg cctcccacgc ggcgttggcg gtcgccggcc gggcccaatt
     1741 cgcgaatacc aggatgccga ccataactaa gaagtacgcc gccgtcttcc agagcgggcg
     1801 ttggccagcg tcgtccggca ggacgaatcc gccggccgcc ttggcttctt cagccttggc
     1861 ccgttcttcc ttgcgataaa tgaagtgcat cgccaagcca atgatgaagc tgaatgcgat
     1921 cgcggcgccg gcgcgggccg cgccgatctg ccagcccagg atacgtgccg tgaggatgat
     1981 cgccagaacg ttgatggccg ggcccgagta aaggaatgcc gatgccggtc ccagccccgc
     2041 gccgcgcgtc caaatgccgg caaacagggg caagaccgta cacgagcaga cggcgagaat
     2101 cgtgccggca accgaggcga ccgtgtaggc caggaacttg ttcgccgtcg gccccaggta
     2161 cttaattacc gagacctgcg acacgaagca tgagattgcc cccgcgataa acagtgccgg
     2221 caccaggcac agaatcacgt ggtgctgggc gtagtcctgg agcatcagca gtgcttcgcg
     2281 cccggcgttg gcgatgcggg tctggccggc ggggatgctc ttgaagggga ggaaatagga
     2341 cagcagaaaa acggcgatca tgagggcgag gagggtcagc gtgcggcggc cctgcgagac
     2401 gacacgccaa accccggtgg cggggccggc tttttggtcc gctggggtat gtacgggcgc
     2461 cgtaacgctc atgatgcgat cgccgcctgc tgggccttga ggt
//
LOCUS       DAOSYS010000371         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_32466_length_2486_cov_3.990539, whole genome shotgun sequence.
ACCESSION   DAOSYS010000371 DAOSYS010000000
VERSION     DAOSYS010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32466_length_2486_cov_3.990539"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="PLU99_15420"
     CDS             <1..1838
                     /locus_tag="PLU99_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOO18481.1"
                     /translation="NHPNIYVVREPMTVVIESGGGRIAVAGFPCIRRRSAAEFAAAVE
                     ATGWRGIAADVRILAVHQTFEGARCGPANFRFRPGPDVVPRDAVPESFDYVAAGHVHR
                     HQTLWRNGPANGGAAEHRGPPIVYSGSPDRIAFAEMGEPKGCVIVEWVPGQVRPCFLE
                     HPVRPMAIASVDVTGLTRKALVEAILQRAAELPACAAAQIRLTGRATKRMLAGLQMTA
                     RLRAARPDLLATISSSAVEWVTERAFFQQAGATPASAFDVLDAPHADIRRVPAAEVGT
                     LPAVCGSYCLYSEEGRLLYVGKGVNLRARLRSHLRGSSATSRFGNWSDQIAHIEVRPA
                     ASELEALLVEAELIRRLTPPFNRQMRAWKHYCYLRLNRRAFGQLEMCSEPRGRVMFGP
                     LHSEAAADRALEALCVWFGLARCEPYDSARHSPLGGPTRLCRRYFLGTCAGPCAERIS
                     REEYQRRLARRDALLAGQPTEDVAQIVAEFEALGSNPEEARERERMLRTLVYLCRTAV
                     LLRRARGLLNRLLVLPGGDGAQTAAVITASGLHLAQFAGGENAAREMWDWHIEKVRAG
                     RQSGSPVLPKPVADTLCTAARALARHPDVYRCQAAIGEEQIGAGK"
     gene            1928..>2486
                     /locus_tag="PLU99_15425"
     CDS             1928..>2486
                     /locus_tag="PLU99_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18482.1"
                     /translation="MPQSPEPQRNPEDVATTGPQNSTSSATAAPPRQRARWKRRLAIT
                     LLIVLVLLAVLVGFAPTIASTSAVYNGILGAVNSNLQGKLQVDELHLSWGGPIAIHGL
                     RVMDPQQQQVLHIPRVVAEAGVWRLITSTMAFGEITLDSPALVIEQNSDGQLTLAQAF
                     ESRRPTPSTPKPAGELPAPEGRLIVK"
BASE COUNT          405 a          769 c          870 g          442 t
ORIGIN      
        1 tgaatcatcc gaacatctac gtagttcgcg agccgatgac cgtggtaatc gagtctggcg
       61 gcggtcgcat cgccgtggcg ggatttccct gcattcgccg ccgcagcgcg gccgaattcg
      121 cggccgcggt cgaggcaacc ggttggcgcg gcattgccgc ggacgtgcgc atactggcgg
      181 tgcatcagac gtttgagggg gcgcgctgtg ggccggccaa tttcaggttc cggcccggcc
      241 cggacgttgt gccgcgcgac gccgtgccgg agagttttga ttacgttgcc gcggggcacg
      301 ttcaccggca tcagacgctg tggcgtaatg gccccgcgaa tggcggcgcg gcggagcatc
      361 gcgggccgcc gatcgtctat tccggctcgc ccgaccgcat tgctttcgcg gagatgggtg
      421 aaccgaaggg ctgcgtgata gtggagtggg tgcctggcca ggttcgtccg tgtttcctcg
      481 agcatccggt gcgtcccatg gcaattgcat cagtggacgt gactggcctg acgcgcaagg
      541 cgctggttga ggcaatattg cagcgggcgg ccgagttgcc ggcgtgcgcc gcggcccaga
      601 ttcgactgac ggggcgggcc acgaaacgca tgctggcggg tctgcaaatg acggcccgcc
      661 tgcgcgccgc gcggcctgac ctgctggcga ccatttcgtc cagtgcggtg gagtgggtta
      721 cagagcgcgc gttctttcaa caggccggtg caacgcccgc atccgccttt gacgtgctcg
      781 acgcgccgca cgctgacatc aggcgtgttc cagcggcgga agtcggcacg ctgccggccg
      841 tgtgcggttc gtactgtcta tacagcgaag aggggcgctt gctctatgtc ggcaaggggg
      901 tgaacctgcg ggcccggctg cgctcgcatc tgcgcggctc ttcggcgacc agtcgcttcg
      961 gcaactggtc ggatcagatc gcgcacatcg aggttcgtcc ggcggcgtcc gaactcgagg
     1021 cgctgctggt cgaggctgag ctgattcgcc ggcttacgcc accgttcaac cggcaaatgc
     1081 gcgcgtggaa acactactgc tacctccggc tgaaccggcg cgcgttcgga cagttggaga
     1141 tgtgttctga gccgcgcggg cgcgtaatgt tcggaccgct gcacagtgag gccgccgctg
     1201 accgggcact tgaggcgttg tgcgtctggt tcggattggc gcgttgcgaa ccatacgatt
     1261 cggcccggca ttcaccgctc ggcgggccga ctcggctgtg ccgccgatat ttcctaggaa
     1321 cgtgtgccgg gccctgtgcg gagcggatca gccgcgaaga gtaccagcgg cggctcgcgc
     1381 ggcgggatgc gctgctggcc ggtcagccga ccgaggacgt tgcgcagatt gtagcggagt
     1441 tcgaggcgct gggctcaaac ccggaagagg cgcgggagcg cgaacgaatg ctgcgaacgc
     1501 tggtttactt gtgtcgcacg gcggttctgt tgcgccgggc gcgcggcttg ttgaatcgct
     1561 tgctggtgct gcccggcgga gatggggcgc agaccgcggc cgttatcacg gccagcggac
     1621 tgcacctggc ccagtttgcc ggcggcgaaa atgcggcccg cgagatgtgg gactggcata
     1681 ttgagaaggt gagggccggt cgccagagcg gatcgcccgt cctgccaaaa ccggtcgcag
     1741 acaccctgtg caccgcggcg cgagcgctgg cgcggcatcc ggacgtctac aggtgtcagg
     1801 ccgctatagg cgaagagcaa attggtgcgg gaaaatgacc gcatcgactg cggcgccagt
     1861 atcattggcc agtgttgggc cgacgatttt ctcgggagcc agcccgcccg ggcgaaggag
     1921 ctaagccatg ccgcaatcac ccgaacccca gcgaaatccg gaagatgtcg ccacaacggg
     1981 cccgcaaaat tcgacttcgt ccgcaacagc ggcgccgccc aggcagcgtg cgcggtggaa
     2041 gcgccggctc gcgatcaccc tgctgatcgt cctggtgttg ttggccgtgc tagtgggatt
     2101 tgcaccaacc atcgcctcaa cttcggcggt ttacaacggc atcctgggcg ccgtgaacag
     2161 caatctgcag ggcaagctgc aagtggacga gcttcacctg tcctggggcg ggccgatcgc
     2221 gatccacggc ctgcgcgtca tggatccaca acagcaacag gtgctccaca ttccacgcgt
     2281 ggtagccgag gccggcgtgt ggcggctgat cacgtccacg atggccttcg gcgagatcac
     2341 tctcgactcg cccgctcttg ttatcgaaca gaacagcgac gggcaactca cgcttgcgca
     2401 agcttttgag tcacgccggc cgacgccctc aacgccgaag ccggccggcg aattgccagc
     2461 gccggagggc cgcctgattg tgaaaa
//
LOCUS       DAOSYS010000372         2476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_32606_length_2476_cov_5.505576, whole genome shotgun sequence.
ACCESSION   DAOSYS010000372 DAOSYS010000000
VERSION     DAOSYS010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32606_length_2476_cov_5.505576"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PLU99_15430"
     CDS             <1..969
                     /locus_tag="PLU99_15430"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOO18483.1"
                     /translation="EQRRFPAQQYRLLASANSAGRTLTWCGRELPVETLRPNSFEGCD
                     LALFSAGAAVSREFGPCAARGGAVVIDNSSAFRMDSDVPLVVPEVNAAAAFQHKGIIA
                     NPNCSTIILAVVLWPLHRVNPIRRAVVATYQAASGAGARALAELESQTRSVLAGQLPR
                     PELFPEPCAFNVFSHNSALAPDGYNLEETKLIRETRKIFGQPVDGPDSIQIAPTCMRV
                     PVMRAHTEAFAIEFTRPMPEEEARTLLAAAPGVRLVDDRERNHFPTPLEATGADEVLV
                     GRIRQDPTLPDGRGLQLLCSGDQLRKGAALNAVQIAELLLSGQASI"
     gene            complement(943..2205)
                     /locus_tag="PLU99_15435"
     CDS             complement(943..2205)
                     /locus_tag="PLU99_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18484.1"
                     /translation="MKPCVLAILRRPEGLFALTARRRAPFEPPQPYSRETEVPARCPY
                     EEVLLPLAQLSAPRVVNRKLLAAWEALRRVSVLNEEVAANVLGIVACTLPDPLADTAL
                     VGPLELDDSPARPRATAAHPRAYRGTIFKPPKPRQPRAHDLTGHLCLHSDEADILARL
                     EPFLPAALGNLEAHWPQEVAATIRAHASGALRAYGQNWSREAAERIPSLPSGLRRYLL
                     YGQRGQSWRQLARVLELWWGLDLEHEADLRLCVALALSRSPGRAGIEWCATALQVAPA
                     RLQFMELLLETRAYERQPAPETTAQVLQADQLCSDKVWRHRMYAVFQLARQLLAISEA
                     DELVCFNVYPEDVATDVRKLFREYDVRFARKLGLCIHDGGSEKPEYEIRTILSHDFWD
                     DGAWRAGRGKVGRRSGHCRGSDARLAGQ"
     gene            complement(2276..>2476)
                     /locus_tag="PLU99_15440"
     CDS             complement(2276..>2476)
                     /locus_tag="PLU99_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18485.1"
                     /translation="RGAHLLIPAAACSGCKNSGNFAGVERRFMRSTCGIAHLRAARPG
                     LVAVYAAVLALAWLPAVARAQA"
BASE COUNT          445 a          852 c          767 g          412 t
ORIGIN      
        1 gagcagcgcc gcttcccggc gcaacagtac cgcttgctgg cctcggcaaa ctcggccggg
       61 cgcacgctga cctggtgcgg gcgcgagctg cccgtcgaaa cgctgcggcc caactcgttc
      121 gagggctgcg atctggcgct attctcggcc ggcgcggccg tcagccgcga atttgggcct
      181 tgcgccgcgc gcggcggcgc ggtggtgatc gacaattcct cggcgttccg gatggattcg
      241 gacgttccgc tggtcgtgcc tgaagtaaac gcggcggccg ccttccagca taagggcatc
      301 atcgccaatc cgaactgctc gaccattatc ctggcggtcg tgctctggcc gctgcaccgc
      361 gtcaacccga ttcggcgagc tgtggtggcg acctaccaag ccgccagcgg cgccggggcg
      421 cgagcgctgg cggagttgga atcgcagacg cgcagcgtgc tggccggcca actcccgcgc
      481 ccggagcttt tccccgagcc gtgtgccttc aacgtcttta gtcacaacag cgcgctcgcg
      541 ccggatggct acaacctcga agaaaccaag ctgattcgcg agacgcgcaa aattttcggc
      601 cagccggtgg acggcccaga ttcgatccag atcgcgccca cctgcatgcg cgtcccggtc
      661 atgcgcgccc acactgaggc gttcgccatc gagttcacgc ggcccatgcc cgaggaagag
      721 gcgcgtacct tgctggccgc cgcgcccggc gtccgcctgg tcgacgaccg cgagcgcaat
      781 cacttcccca caccactcga agcaaccggc gcggacgagg tcctcgtcgg gcgcatccgg
      841 caggatccga ctctacccga cggccgcggc ctgcaactgc tgtgcagcgg agatcagttg
      901 cgaaagggcg cagcgctgaa tgcggtgcag attgcggaac tactactgtc cggccaggcg
      961 agcatctgag ccgcggcaat gtccgctcct gcggccgacc ttgccgcgac ccgcgcgcca
     1021 agctccgtca tcccagaagt cgtgcgacaa tatggtgcga atctcgtact ctggcttttc
     1081 agacccgccg tcgtgaatgc acaagccgag cttgcgggca aatcgtacgt cgtattcacg
     1141 aaagagcttc cttacgtccg tagcgacgtc ctctgggtag acgttgaagc aaaccagctc
     1201 gtcggcttca gagattgcca ataactgtcg tgcgagctgg aagacggcgt acatcctgtg
     1261 gcgccagact ttgtccgaac acaactggtc cgcttgaagc acttgggccg ttgtctccgg
     1321 cgcaggctgc cgttcatatg ccctcgtctc gagcagcaat tccatgaact gcaggcgcgc
     1381 aggtgctacc tgaagcgccg tggcacacca ctcgattccc gcgcgtccgg gggaacggct
     1441 cagagccaac gccacgcaga gacgcaaatc cgcttcgtgc tccaagtcca aaccccacca
     1501 caattcgagc acccgcgcaa gctgccgcca ggattggccg cgctgaccat acagcagata
     1561 acgccgcagg cccgacggca gacttggaat gcgctccgcc gcttcgcgcg accagttctg
     1621 cccgtacgcc cgtagcgcgc ccgacgcgtg cgcgcgaatt gtcgcggcca cctcctgcgg
     1681 ccagtgcgcc tccaggtttc cgagtgctgc cggcaagaac ggctctaatc tcgccaggat
     1741 gtccgcctca tctgaatgta agcacaaatg cccggtcaga tcatgtgccc gcggctgacg
     1801 cggcttggga ggcttgaaaa tcgtcccgcg ataagcgcgc ggatgggccg cagttgcccg
     1861 cgggcgcgcc ggcgaatcgt ccagttcaag cggaccaacc agggcggtgt cggccaaggg
     1921 gtccggcaac gtgcacgcca caatgccgag aacgttcgcc gcgacttcct cattcagcac
     1981 agacacacgc cgcagcgctt cccacgccgc caggagcttg cgattgacca cccgcggcgc
     2041 cgacaactgt gccagcggca gcagaacctc ttcatacggg caccgcgccg gcacttcagt
     2101 ttctctagag taaggttgcg gcggctcaaa tggcgcccgg cgacgggccg tcagggcgaa
     2161 tagtccttcc ggccggcgca ggatggcgag gacacacggt ttcattgttc cgctcggagc
     2221 gagattcttg ttggtccggg ctggcccggg cttgcagtct acgccagcgc actgtttacg
     2281 cctgcgcgcg tgcgacggcc ggcagccacg ccagggccag caccgcggca tacacggcga
     2341 ccagcccggg gcgcgcggcg cgcaaatgtg ctataccaca tgtcgaacgc atgaaacgcc
     2401 tctcaactcc tgcaaagttt cccgagttct tgcagccgct gcaagcggcc gctgggatca
     2461 gaaggtgagc accgcg
//
LOCUS       DAOSYS010000373         2456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_32951_length_2456_cov_4.181175, whole genome shotgun sequence.
ACCESSION   DAOSYS010000373 DAOSYS010000000
VERSION     DAOSYS010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32951_length_2456_cov_4.181175"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLU99_15445"
     CDS             complement(<1..227)
                     /locus_tag="PLU99_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOO18486.1"
                     /translation="MEHFADRLTAAVAARATPAIVGLDPVLERLPAELRRHANTASAA
                     AEATEKFCRAVLDAVAPVVPGVKINSAFFEV"
     gene            complement(395..868)
                     /gene="bcp"
                     /locus_tag="PLU99_15450"
     CDS             complement(395..868)
                     /gene="bcp"
                     /locus_tag="PLU99_15450"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOO18487.1"
                     /translation="MRPGMPAPDFALADETGEMHTLASYRGRRVVLYFYPRDNTPGCT
                     REACGFRDALDEFESRNAVVLGVSPDASESHAAFAKKYRLTFPLLADESHAAAERYGA
                     WGEKRGSDGQLRLGILRSSFVIDEAGRIAQVFRQVNPAGHEQEILDFLDQMPWQS"
     gene            complement(1881..2336)
                     /locus_tag="PLU99_15455"
     CDS             complement(1881..2336)
                     /locus_tag="PLU99_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18488.1"
                     /translation="MIEPHTSTICATVAVEGRPLQPRPGLVGQFTEPELTPEEKEALC
                     IEPEPINWLEFYVPKPAVNLVSDDLGGTAVNIWGLGGDVSGITFARQGMGVGFQHDPV
                     SGASIQAVRVAESGARKPPVGEVGLGEGGASVGEDRARKVAAHDERCQP"
BASE COUNT          420 a          775 c          756 g          505 t
ORIGIN      
        1 aagacttcga agaacgccga gttgattttg accccgggga cgaccggggc gaccgcgtcg
       61 aggaccgcac ggcagaactt ttcagttgcc tcggcggccg ctgaagcggt attagcgtgt
      121 cgacgcaatt cggcggggag gcgttccaga acggggtcga gcccgacgat ggcgggcgta
      181 gcgcgcgccg cgacggcggc cgtcagccga tcagcaaagt gttccatagt gcgtcatatt
      241 ggactgtggc tcgtagagtg gctagtggac gggctgcgag ttccgctatt cactgcccac
      301 attcactcaa ctcaccattt actaataacc attcaccggc gccttgcacg ccgctccatt
      361 agggcacagc cagccgctgc gccacgggcg tatcctacga ttgccacggc atttggtcga
      421 ggaaatccag gatttcctgc tcgtggcccg ccggatttac ctggcggaac acttgcgcga
      481 tccgccccgc ttcgtcgatc acgaagctgg agcggagtat gcccagccga agctgcccat
      541 cgctcccgcg cttctcgccc caagcgccgt agcgctcggc cgccgcgtgg ctctcatcgg
      601 ccagcagcgg gaacgtaagg cggtacttct tggcgaaagc cgcgtggctt tcagacgcat
      661 cgggcgagac gcccaggacg accgcgttgc gtgattcgaa ctcatccagc gcgtcgcgaa
      721 agccgcaggc ttcgcgcgtg caaccgggtg tattgtccct ggggtaaaag tacagcacca
      781 cgcggcggcc acgatacgaa gcgagcgtgt gcatttcgcc ggtttcgtcc gcaagcgcaa
      841 agtcgggcgc cggcatgcca ggcctcaact ggacatgcgc tgagggcgaa ccggcgtgct
      901 gcgcggccac aggagctgag tgtgaggctc tcctgcggcc gcggggcaag tcctcgtctt
      961 cttcctcctc ttctccttcc tcctcgctgc ggggctcgtc gggctcgtag agggtgggcc
     1021 cttcctccat ttcggattcg acgtccagca ctgaggcgcc gccgggtcct aactcttcga
     1081 agtcgggcat ggctgtgctg ggttcctcag ggtacgggcg cagctcctcg ggtggagggg
     1141 gggctggttt aggcgggcgt ggagccacag gcttcctgac cggcttgcgg gccttcttag
     1201 cggccgcttt cggagccttc ttgggagccg ctttccgctt cgccttcttg gcggcctttg
     1261 gtgcagcttt cttgacggcc ttcttggcaa ccttcttctt ggccgtcggc cggcgggcac
     1321 cgcgggcagg agttttcttt ttggcagtct tagccttctt tttcgccacc ttggaagttt
     1381 tcttagcgcc agctttcttt ttctttgcca tcgcgcaccc tcagtagcaa actagcatga
     1441 gtaaagcgga tccggtgaac ttcgcgcgta aacgcgaaac tctcacgcct ctgccgcgcg
     1501 acacgatcgc gcgcccgctg caccgttccc gagtccgtcg cggccgcgct ttgaactact
     1561 caccaatccg cgacggtgcg tcatataata aacgcgtttt gtgcgtttcg cgacaatagg
     1621 gcgcacatca aatcttggca gcgctgggca gaggtattgt gcatcttcgc agtcgtcata
     1681 atcgatcggc ggaatgcagt gctggtcagt tgtctgactg gagagatttc gagattcagc
     1741 gttctttccg taaccgcgca cggcctttga aacggcgctg gcgcgattgc cataagctct
     1801 tgtgcgatag agggttacag actcttgcga gcggccaaat ttgcctgtgt aggaggtgcg
     1861 tcgagaacac gcccggcaga ctacggttgg caccgttcgt catgcgcggc cactttgcgg
     1921 gcccgatcct cgccgacgct agctccacct tcgcccagac ccacttctcc aacgggcggt
     1981 ttccgcgcgc ccgattccgc aacgcgaacc gcctgaatgg atgcaccaga gaccggatcg
     2041 tgttgaaaac cgacgcccat gccctgacgt gcgaacgtga tacccgaaac atcgcctccc
     2101 agaccccaga tgtttacagc ggtgccaccg agatcatcac tgaccaagtt gacggccggc
     2161 ttcggtacgt agaactcgag ccagttaatg ggctcgggct cgatgcacag agcctccttc
     2221 tcctcgggcg tcaactcggg ttctgtgaac tggcccacca agcccggccg cggctgcaac
     2281 gggcgccctt caacggctac cgtagcgcag atcgtactgg tgtgcggctc aatcactatg
     2341 agttcatcgg gcgagggcgg cacgtcaagg gtcgagcacc acggttgtgc gccacgcgtc
     2401 gggcacgccg gcgctgccga aggcgaggcc cgccagcgtg gaggcgaaca tgagct
//
LOCUS       DAOSYS010000374         2447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_33089_length_2447_cov_4.450669, whole genome shotgun sequence.
ACCESSION   DAOSYS010000374 DAOSYS010000000
VERSION     DAOSYS010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33089_length_2447_cov_4.450669"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLU99_15460"
     CDS             complement(<1..1155)
                     /locus_tag="PLU99_15460"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOO18489.1"
                     /translation="MRLKVVAATFGVCLAGLLAPPVRVCAGDMGEPDGMVLIPAGEFQ
                     MGDTFGDGHTDERPRHPVYVDAFYIDTYEVTNQQYCDGLNWAWAQGGLITVTDGVVYQ
                     HGSGTSYPYCDTTTSTPISRITWDGSTFGVTPGKEDHPMVRVSWYGAAAYCNWRGAQE
                     GRPLCYDLSTWTCNFVHGGYRLPAEAEWEKAARGGSAGHRFPWSDQETIQHARSNYFS
                     SPVFAYDTSPTRDSHPCWGSGSIPFTSPVGFFTGALQYQADWDWPGSLTSFQTASGIN
                     GYGLHDMAGNVWEWCNDWYSAAYYSSSPYDNPRGPASGIHRLIRGGSWDGHANLCRVA
                     DRGDYPPSYCYNYFGFRCALGIVDYSADLNGDGYVDAHDFAVFTTCMAGPD"
     gene            1638..>2447
                     /locus_tag="PLU99_15465"
     CDS             1638..>2447
                     /locus_tag="PLU99_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18490.1"
                     /translation="MPLSSLILLILHSAQWPTVSPAELKAVDLNAGWVKVAKTEGTTL
                     PNDTELVLAAEVVPIGRFRLTEQGADHATFARAEPTLHAGAGRLRAWFVSPRPSPQLW
                     EKWPPEADFMARIERVGPGMRGVWIDAGATSGIQPGMSWWQRIDGQPVARFDVLSVEA
                     DACYCSVARLAADAPIRRHDRVALWPSPGRRRNGTAASAVSYIERSEERTIVWVAGPR
                     GVEVPPEPHVDFFHNAHYVAHGVVESADNLFWYARLIEGGANGGGDRAEQPP"
BASE COUNT          404 a          809 c          784 g          450 t
ORIGIN      
        1 gtccgggccg gccatgcagg tcgtgaagac tgcgaagtcg tgggcatcca cgtacccgtc
       61 gccgttcaag tcggcgctgt agtccacaat tcccaaggca caccggaacc cgaagtagtt
      121 gtagcaatag cttggcggat agtcgccgcg atccgcaacg cggcagaggt tcgcgtgccc
      181 gtcccaactg ccgccgcgaa tcagacggtg gatccccgac gctggcccac gcgggttgtc
      241 gtacggactg ctgctgtagt aagccgcgct ataccagtca ttgcaccatt cccatacatt
      301 gcccgccatg tcatgcagac cgtagccgtt gatgccgctc gcggtctgaa agctggtcaa
      361 actgccgggc cagtcccaat ccgcctggta ctgcagcgcg ccggtgaaga agccgaccgg
      421 actggtaaag ggtatgcttc cgctgcccca acacgggtgt gagtcccgcg tcggactggt
      481 gtcgtatgca aacacgggtg aactgaagta gttgctccgg gcgtgctgaa ttgtctcctg
      541 gtcggaccag gggaaacggt gtcccgcgct gccgccccgc gccgctttct cccactcggc
      601 ctcggccggc agccggtaac ctccgtgcac gaagttgcac gtccaggtcg ataggtcgta
      661 gcacagcggc cggccctcct gcgcgccgcg ccagttgcag tacgcggccg cgccgtacca
      721 gctcaccctc accatgggat ggtcctcctt gccgggcgtc acgccgaagg tgctgccatc
      781 ccaggtaatg cggctgatcg gggtgctggt agtcgtgtcg caatacgggt aactcgtgcc
      841 gctcccgtgc tggtaaacca cgccgtcggt caccgtgatc aggccgccct gagcccaggc
      901 ccagttcagc ccatcgcagt actgctggtt cgtcacctcg tatgtgtcga tgtagaaggc
      961 gtcgacgtac actggatgcc tgggccgctc gtcggtgtgg ccatcgccga acgtgtcgcc
     1021 catctggaac tcgccggccg ggataaggac catgccgtcg ggctccccca tgtccccggc
     1081 gcagacgcgc accggaggcg ccaacaatcc tgccaagcag actccgaacg tcgccgcgac
     1141 gaccttcaat ctcatgaggc ttccttcctg cttcgcgaac gcgcgggcca cgttcggaca
     1201 cggcctcgcg tacatccttt ccccgctcat cggggatttt agcgaggcta agtcgcggca
     1261 ccgaccgagt ttccggccgg gtgatcgaaa acgcaccggg cgagcgctga gggtcgctcg
     1321 gcggctgcct tggagaacgg gggcccgcct gctttttttt ctgatgaagc gcgaaccgac
     1381 ccgccgatca ttcgtaagga agttagaaaa cgagaagttc tcctcctagc ctccttctcc
     1441 aatgagaccc gaatgcggcc gccgtgtggc ggccgcagtc gtttatgggt tgagcctgcc
     1501 ggcagcggct ccgcacgcct gacccgcctt ggggtcggca cgtctcgacg gcacgtcggc
     1561 gagcgttatt atttgaccgt ggcgagttgc ctggttgcgc cgctgccgtg tcggcggact
     1621 cgacttttgt gtcataaatg cccctcagca gccttattct cctgattctg catagcgcac
     1681 aatggccgac ggtttcgccg gcagagctga aagccgtcga tctcaacgcc ggttgggtca
     1741 aggtggccaa aaccgagggc acgacgctcc cgaacgatac tgaactcgtc ctggcggccg
     1801 aagtggtgcc gatcggccgc tttcggttga ccgaacaagg cgccgatcac gccacgttcg
     1861 cgcgggcgga accaacgctc catgccggcg ccgggcgtct gcgagcgtgg ttcgtttcgc
     1921 cccgcccatc gcctcaattg tgggagaaat ggccgcctga ggcggacttc atggcgcgga
     1981 ttgagcgcgt ggggccgggg atgcgcggcg tctggatcga cgccggcgcg accagcggca
     2041 ttcagcccgg catgagctgg tggcaacgca tcgacggcca gccggtggct cgcttcgacg
     2101 tgctctcagt cgaagccgat gcctgttact gctcggtggc gcgcctggcg gccgatgcgc
     2161 cgatccgccg ccacgatcgc gtcgcgctct ggccttcccc cggccggcgc cgcaatggga
     2221 ctgcggccag cgcggtcagc tacatcgaac gcagtgaaga gcgaacgatc gtctgggtgg
     2281 ccggcccgcg cggagtggaa gttccgcccg agccgcacgt tgatttcttt cacaacgcgc
     2341 actacgtcgc ccacggcgtc gtcgagtcgg cggacaatct gttctggtac gcgcggctga
     2401 tcgaaggcgg tgcaaacggt ggaggcgatc gcgccgagca gccgccg
//
LOCUS       DAOSYS010000375         2445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_33134_length_2445_cov_4.625105, whole genome shotgun sequence.
ACCESSION   DAOSYS010000375 DAOSYS010000000
VERSION     DAOSYS010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33134_length_2445_cov_4.625105"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLU99_15470"
     CDS             complement(<1..360)
                     /locus_tag="PLU99_15470"
                     /inference="COORDINATES: protein motif:HMM:NF040245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18491.1"
                     /translation="MITATEPNYSFAAAFEEFEGRLDGGRPWLRELRQAALARFLERG
                     FPTTRDEDWRFTNVSVLQRTPFRPAGVCDSPAQRATLLALPWDVGGEQLILVNGRYAP
                     DLSTLSAENGLRCGNLAS"
     gene            complement(367..1158)
                     /gene="sufC"
                     /locus_tag="PLU99_15475"
     CDS             complement(367..1158)
                     /gene="sufC"
                     /locus_tag="PLU99_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOO18492.1"
                     /translation="MLVIENLHVAVDGQEILKGVDLMVGAGEVHSIMGPNGSGKSTLA
                     QVIAGRDTYQITAGRILYRGRDLTELSPEERACEGIFMAFQYPVEIPGVSTSYFLRSA
                     LNAQRKYRGLEEVDALDFLTLVKERIRLLGMDERLLNRALNEGFSGGEKKRNEIFQLA
                     VLEPRLAILDETDSGLDIDALKVVADGVNRLRSPERAFVVVTHYQRLLNYIVPDYVHV
                     LAGGRIVRSGGKELALELEQRGYGWLSDEQAIEAVAGQAAAERAG"
     gene            complement(1202..>2445)
                     /gene="sufB"
                     /locus_tag="PLU99_15480"
     CDS             complement(1202..>2445)
                     /gene="sufB"
                     /locus_tag="PLU99_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOO18493.1"
                     /translation="TWQNVHYPPIDYQAIFYYSAPKQKTGPKRLEDVDPKLLETYRRL
                     GIPLEEQKRLAGVAVDAVFDSVSVATTFKEKLAELGIIFCSFSEAVQNHPELVRRYLG
                     SVVPYSDNYFATLNSAVFSDGSFAYIPKGVRCPMELSTYFRINAAQTGQFERTLIIAE
                     PGAHVSYLEGCTAPMRDENQLHAAVVELVALDDATIKYSTVQNWYPGDAQGRGGIYNF
                     VTKRGKCLGRNSRISWTQVETGSAITWKYPSCILQGDNSVGEFYSVALTNNYQQADTG
                     TKMIHIGKNTSSYIVSKGISAGHGQNTYRGMVKILKGAEYARNFTQCDSMLIGEHCGA
                     HTFPYIEVKNSTAHMEHEATTSKIGDDQMFYCHQRGISTEDAVSMIVNGFCKEVFREL
                     PMEFAVEAQKLLEVSLEGSVG"
BASE COUNT          450 a          807 c          687 g          501 t
ORIGIN      
        1 gctcgccagg ttgccacatc gcaggccgtt ttcggccgac aatgtcgaga ggtccggcgc
       61 gtaacggccg ttcaccagga tcaattgctc gccgcccaca tcccagggca acgcaagcag
      121 cgtcgctcgc tgggccggcg agtcacagac cccagccggc ctgaatggcg tgcgctgtag
      181 cacgctgacg tttgtgaagc gccagtcctc atcgcgcgtg gtcggaaagc cacgttccag
      241 gaagcgtgcc agtgccgcct gccgcagctc ccgcagccac gggcggccgc cgtcaagccg
      301 accttcgaat tcctcaaaag cggccgcgaa actgtagttc ggttccgtcg cggtaatcac
      361 gccgcctcac cctgcccttt ccgccgccgc ctgtcccgcc actgcctcga tggcctgctc
      421 gtcgctcagc cagccgtaac cccgctgttc gagttccagg gccagctctt tgccgccgga
      481 gcgcacaatt cgcccgccgg ccagcacgtg tacatagtcg ggcacaatgt agttaagcaa
      541 gcgctgataa tgtgtgacga cgacgaatgc ccgctccgga ctgcgcaagc gattgacgcc
      601 gtcagccacg accttcagcg catcgatgtc cagacccgaa tcggtctcgt ccagtatggc
      661 caggcgcggc tcgagcaccg ccaactgaaa aatttcattg cgtttcttct cgccgcccga
      721 gaaaccttcg ttaagcgcgc ggttcagcag tcgctcatcc attcccagca gccggatgcg
      781 ctccttcacc aacgttagaa aatcgagcgc gtcgacctct tccagcccgc ggtactttcg
      841 ctgagcgttt agcgccgagc gcagaaagta gctggtgctg acgcccggga tttcgaccgg
      901 gtactggaac gccatgaaaa tcccctcgca ggcccgctcc tccggcgaca actcggtcaa
      961 atcgcgcccg cggtaaagta tgcgtccggc ggtgatctgg taggtatcgc ggccggcgat
     1021 aacctgcgcc aaggtgctct tcccggaacc attcggcccc atgatagagt gcacctcgcc
     1081 ggccccgacc atcaggtcga cccctttgag tatctcctgt ccatcaaccg cgacgtgcag
     1141 gttttcaatt acaagcattc cagctccgtt ttctatgcgc ttactttgcc ctcacgcact
     1201 cttagccgac acttccttcg agactgacct ccagcagctt ctgggcctcg accgcaaact
     1261 ccatgggcaa ttcgcggaac acttccttgc agaagccgtt gacaatcatc gaaaccgcgt
     1321 cctcggtcga aatgccgcgc tggtggcaat agaacatctg gtcatcgccg atcttcgagg
     1381 tagtggcctc gtgttccatg tgggcggtgc tgttcttgac ctcgatatac gggaaggtgt
     1441 gcgccccaca gtgctcgccg atcagcattg aatcgcactg cgtaaagttc cgggcatact
     1501 cggccccctt cagtatcttc accatcccgc ggtaggtgtt ctgcccgtgt ccggccgaaa
     1561 tgcctttcga cacgatgtaa ctgctcgtgt tcttgccgat atggatcatc ttggtgcccg
     1621 tatcggcctg ctgatagtta ttggtcaggg cgacgctata gaattcgccg accgaattgt
     1681 cgccctgcag gatgcaactg ggatacttcc aggtaatcgc cgagccggtt tccacctgcg
     1741 tccaggagat gcgcgagttg cggcccaggc atttgccgcg cttggtaacg aagttgtaga
     1801 tgccgccacg gccctgggcg tcgcctgggt accagttctg cacggtcgag tacttgatcg
     1861 tggcgtcgtc cagcgccacc agttccacca ccgccgcgtg aagctggttc tcgtcgcgca
     1921 tcggcgccgt gcaaccttcc aggtagctca cgtgggcccc gggctcggca atgatcagcg
     1981 tgcgctcgaa ttgcccggtc tgggccgcat tgatacggaa gtaagtcgag agctccatcg
     2041 ggcaacggac gcccttgggt atgtatgcga acgaaccgtc gctgaacacc gctgagttca
     2101 gcgtcgcgaa gtaattgtcc gagtacggca ccaccgagcc gagataacgc cgcaccagtt
     2161 ccgggtgatt ctgcacggct tcggagaacg aacagaagat gatccccaac tcggccagct
     2221 tctccttgaa cgtggtcgcg accgagacgc tgtcgaacac cgcgtcgacc gccacgcccg
     2281 ccagccgctt ctgttcctcc agcgggattc ccagccgccg ataggtttcc agcagcttcg
     2341 gatcgacgtc ctccagccgc ttggggccgg tcttctgctt cggcgccgag tagtagaaga
     2401 tggcttggta atcgatcggc ggataatgca cgttctgcca ggtcg
//
LOCUS       DAOSYS010000376         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_33386_length_2429_cov_5.388795, whole genome shotgun sequence.
ACCESSION   DAOSYS010000376 DAOSYS010000000
VERSION     DAOSYS010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33386_length_2429_cov_5.388795"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLU99_15485"
     CDS             <1..627
                     /locus_tag="PLU99_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18494.1"
                     /translation="ILQRVWAPNELKQRLGDLTAELKPAARIFCTGPVHLPEVGDLRF
                     EPVIITPTRFVWRKLLFVPFALLIMAFWLAQVTHLLKLPFTLPLGAFGYVLSMGGIAL
                     VMWIWRTTVRPSYVRLAPGMVQVLEYRLRGGKPVIRSYPMLPGTLVVVRQGKKERRPS
                     VVQLVRGDQADVLPVSQMKDSDEAMEHIWQALLSTAPTPPLNEEELVG"
     gene            complement(751..1905)
                     /locus_tag="PLU99_15490"
     CDS             complement(751..1905)
                     /locus_tag="PLU99_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18495.1"
                     /translation="MVESEGTAQAGATPVCSPPGHVQIARGTCHILFAYDVGQAIDLE
                     DAERRIAALTQREAVRRKRRAPRFFEYQPPPLRVTQTVQPLTIGAHSSLNTADAVIYD
                     FGAVSVAFSIPLSGSLTDVLALSGAIWDQTQLVDHSRIFVESLLRTIEPAVSMPQVSQ
                     LVEDYIIYQLDAFTPPQRPADVIAANAACLAQILRAECEPLSRQERDDALAAQLSFGL
                     DDATVVDWNAALICDPDAYDVRAVLEFANVELLELRFLDDRLDDVVAQAYAALPRRWG
                     RRRGFGAKDLWHIARLQMDSAMLFEGVNNAVKLLGDQYLARVYRTASNRFHLNDWDSS
                     ILRKLQTIESIYDKISDRQANRRIEALEWIIIILIAVEIVLYLAMSFTPH"
     gene            2227..>2429
                     /locus_tag="PLU99_15495"
     CDS             2227..>2429
                     /locus_tag="PLU99_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18496.1"
                     /translation="MFKLRALIVASALIVALPLTACGRNSNGAMRATDTSPPPRTLST
                     ASFETTPAPALESGRTYEPAPPPG"
BASE COUNT          460 a          761 c          746 g          462 t
ORIGIN      
        1 attctgcagc gggtttgggc gcccaacgag ctgaagcaac ggctgggcga cctcacggct
       61 gaacttaaac cggcggcccg catcttctgt acggggccgg tgcaccttcc cgaagtaggc
      121 gacctgcgat tcgagcccgt gatcattacg cccacacggt ttgtgtggcg caagctcctt
      181 ttcgtgccct tcgcgctact gatcatggca ttctggctgg cccaggtcac gcacttgctc
      241 aagcttccct tcacgctgcc gctgggagcc ttcgggtacg tactttcgat gggaggcata
      301 gcgctggtaa tgtggatctg gcgaaccacc gtgcggccat catacgttcg gctggcgccc
      361 ggcatggtcc aggtgctcga gtaccggctg cgcggcggga aaccggttat ccgcagctat
      421 ccgatgctgc cgggtacgct ggttgtagtg cgacaaggca agaaagagcg tcgcccgtcg
      481 gttgtgcagc tcgtacgcgg cgaccaggcg gacgtgttgc cggtgtcgca gatgaaagac
      541 tccgacgaag ccatggagca tatctggcag gcgctgctct caaccgcccc gacgccgccg
      601 ttgaacgaag aggaactggt gggctgagcc gggtctgacg ttcgccagtg tcgtggtcgc
      661 ccttccggcg ccaatcctcc tcgccccctt cctattgagg ctcagacctc aacagtggca
      721 cacggcgagc gcccacgcgc ctgccgtcgc tcaatgcggt gtgaaactca ttgccaagta
      781 aagcacgatc tccacggcaa tcagaataat gatgatccac tcgagtgctt cgatgcggcg
      841 gttcgcctgg cggtcggaaa tcttgtcata aatgctttct atggtttgca gcttgcgcaa
      901 gatgctgctg tcccaatcgt tcaaatggaa acggttggag gcagtgcggt aaacgcgcgc
      961 caggtactga tcacccagca gttttacagc gttattaacc ccttcaaaca gcattgcgct
     1021 gtccatctgc agccgggcga tgtgccaaag gtccttggcc ccgaagccgc gccggcggcc
     1081 ccagcggcgc ggcaaggcgg cgtaagcctg ggccacaacg tcgtcgagcc ggtcgtccag
     1141 gaagcgcagc tccagcaact cgacattcgc gaattcgagc acggcccgca cgtcgtaggc
     1201 gtcgggatcg cagatcagtg cggcgttcca gtccaccacc gtcgcatcgt ccaggccgaa
     1261 cgagagctgt gccgccaggg catcgtcgcg ctcctgcctg gagagcggtt cgcactccgc
     1321 ccgcagaatc tgggccaggc acgccgcgtt agcagcgatg acgtcggccg gtcgctgcgg
     1381 cggcgtgaaa gcgtcgagct ggtaaataat gtagtcctcg accaactggg agacctgggg
     1441 catggagacg gccggttcga ttgtcctgag caggctctcg acgaagatcc gcgaatgatc
     1501 gacaagctgc gtttggtccc aaatggcgcc actcagcgcc aatacgtccg tcagcgagcc
     1561 ggaaagcggg atgctgaacg ccaccgaaac cgctccgaaa tcatagatca cggcgtcggc
     1621 ggtattcaga ctcgagtgcg cgccgatggt gagcggctgg acggtctgtg tcacgcgcag
     1681 tggcggcggc tgatactcga agaagcgcgg cgcgcgccgt ttgcggcgca cggcctcgcg
     1741 ctgcgtcaag gcagcaatgc gccgttcggc atcctccagg tcgattgcct ggccgacgtc
     1801 ataggcgaac agaatgtgac acgttccgcg ggcgatctgg acgtggcctg gggggctgca
     1861 gacgggcgtc gcgccggcct gggctgttcc ttccgactcc accatgagtt ctcactcgac
     1921 agttcccggc caagtctagg cgcgctgcgg cacgctgact gtgccccagc ctccattaca
     1981 actctgcaat atctgtccac ctgcgatgcc caaacgcacg ggtgccatgc ccaagctcta
     2041 agcgcgggca tgccgcgtta ggagtcacga cttcccggcc ggtccagcgg acgcacagat
     2101 ccaatgacct tcattacaat ttacgttagc tgtaatttag atatctgtat tggggcattc
     2161 agtattacgc gcgatagtcc tgattgagcc accggttggg aatgatgtgg taatcaggaa
     2221 aaagatatgt ttaaactccg cgcactgatc gtcgcttccg cgctaatagt cgcccttccg
     2281 ctgaccgcct gcggcagaaa ttcgaacggg gcaatgcggg cgaccgacac gagccccccg
     2341 ccgcgcacgc tgtcaacggc ctcgttcgaa actacacccg cgccggccct tgaaagcggg
     2401 cggacctacg agcccgctcc gccgcctgg
//
LOCUS       DAOSYS010000377         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_33390_length_2429_cov_4.149115, whole genome shotgun sequence.
ACCESSION   DAOSYS010000377 DAOSYS010000000
VERSION     DAOSYS010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33390_length_2429_cov_4.149115"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="PLU99_15500"
     CDS             complement(<1..2372)
                     /locus_tag="PLU99_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18497.1"
                     /translation="MGIGDIGQLYREHPELAERPECNLALGAARSALAETGAALELLD
                     AAAEGFAARRENDRLVVSLVALADTWSRHNEWEITPARFGISKPKDTAQARKVRREQI
                     ARLRERVAALPDSTAGLAQIDLLIAQDLLQDADTAPAGQQLLEQLAAATELTPPVVAA
                     TTALAERYEAERRWRDASKLYERLTRDGDQVVSQQASQKLAAITSPQLRLAVPAASPS
                     GRAVTLDVAARNVTAVQVEVRRLDLQSWLTARQGRLSEAHLPVAGSLQFSRQINTPVA
                     QPHDWWESASAGPLEFEAPPGAYVVCVETADQSHLEPLKRLLIVSDLAAVAWVGSEQA
                     VIWGGKNPLSTTAPEGLATLTGDFWMAGSFVPTQPQFTAGVAPMDLPPEARVLRDRRW
                     VCLLRAGEHLALCRGELPQDSGGPKVAFTGGPAEPRPGDILTLAGLVLDHGVKGQSTP
                     AEWQLEIRNTLDEALATLPLKPTAAGMFSVDLPVPESWSGEHLRFVVRRDQRVAENLA
                     PHLLIGVAQAGREEFVICPANSPTWPCDAKSLTASINAWLPWGAPASLTQLSSVLRVV
                     RLPDETNDFQPIASDALRIPGWHGHLPVTTRGPAGFELPLPLDLLDMPPGPTAGGLWV
                     TFSAWNGQQTKTLTEFLCAAEPVHAWIQTDPAELVAGQPVQVRLGWFDPARRTGLMPA
                     SATVHCEGQPPAELQFSAARGSQSAMWHPPQPGEYDIEVLIPRLDEPPVAVHRRVSVH
                     HANVTSLQPLNADTQLASETGFQVNTKANVRLTAEYTDRDRPGAIQVRLEG"
BASE COUNT          378 a          790 c          830 g          431 t
ORIGIN      
        1 ccctcaagcc gcacttggat cgcgccgggc cggtcgcggt cggtgtattc ggctgtgagg
       61 cggacgttcg ccttggtatt gacttgaaaa ccagtctcac tggcgagctg agtatcggcg
      121 ttgagtggtt gaagactggt cacattggcg tggtggacgc tgacgcgacg gtgaactgca
      181 acgggcggct cgtccagtct gggaatgagt acctcgatgt catactcgcc gggctgcggc
      241 ggatgccaca ttgctgactg gctgccgcgg gccgcggaaa actgtaactc ggctggaggc
      301 tgtccctcgc agtgcacggt ggccgaggcg ggcatcaagc cggtccttcg agccggatcg
      361 aaccagccca gccgcacctg gaccggctgg ccggcgacga gttccgccgg gtcggtctgt
      421 atccaggcat ggacgggctc ggcggcgcat aggaattcgg tcagcgtttt cgtttgctgg
      481 ccgttccagg cgctgaatgt aacccacagg ccgccagcgg ttgggccggg cggcatatcg
      541 agcaagtcga gcggcaaggg gagctcaaag cccgcaggcc cgcgcgtggt taccggcaga
      601 tggccatgcc agccgggaat tctgagcgcg tcgcttgcga ttggctgaaa atcgttcgtc
      661 tcatctggaa ggcgcacgac gcgcagcacc gaggatagtt gcgtcaggct cgccggcgcg
      721 ccccacggca gccaggcatt gatgctggcc gtcaggctct tagcatcgca cggccaggtg
      781 ggggagttcg cgggacaaat gacgaattcc tctcgacccg cctgtgcgac gccgatcaga
      841 agatgcggcg ccaggttctc cgccactcgc tggtcgcggc gcacgacaaa gcgcaggtgc
      901 tcgccgctcc agctctcggg aactggcagg tcgacggaga acatccccgc cgcggtgggc
      961 tttagcggca gcgtcgccag ggcttcgtcc agggtgttgc gaatctcgag ctgccattcg
     1021 gcgggcgtgc tctgcccctt tacgccgtgg tccagcacga gcccggcgag tgtcaggatg
     1081 tcgcccggcc gcggctcagc aggcccgccc gtgaaggcta ctttcggtcc gccggagtcc
     1141 tgcggcaatt ctccgcggca tagcgccaag tgctcgcccg cgcgtaacag gcacacccat
     1201 cggcggtcgc gcagtacgcg cgcttcgggc ggtagatcca tcggcgccac gccggccgtg
     1261 aattgcggct gcgtcggcac aaacgaaccg gccatccaga aatcgcccgt cagcgtggcg
     1321 aggccttccg gggcagtagt gctgagcggg ttcttgccgc cccaaatgac ggcctgctcg
     1381 ctgccgaccc aggcgacggc cgccaggtcg ctcacgatca gcaggcgctt cagcggctca
     1441 aggtgggatt gatccgcggt ttcgacgcag accacgtagg cacccggcgg ggcctcgaat
     1501 tccaaggggc cggccgaggc gctttcccac cagtcatgcg gttgcgccac cggcgtattg
     1561 atttgccttg agaattgcag cgatccggcg accggcaagt gcgcttcgct caaccgaccc
     1621 tgccgtgctg taagccagct ttgcaaatcc agccggcgaa cttccacctg aacggcggtg
     1681 acgtttcgcg ctgcaacgtc gagcgttacc gcgcgcccgc tggggctggc ggccgggact
     1741 gccagacgca gttgcgggct ggtgatggcc gccagtttct gactcgcttg ctgcgatacg
     1801 acctggtcgc catcacgcgt caaacgctcg tatagcttgg aggcgtcgcg ccagcgtctt
     1861 tcggcctcgt agcgctcggc cagggccgtc gtggccgcga cgactggcgg cgtcaattcg
     1921 gtagcggccg caagctgttc caggagttgt tggccagcgg gtgcggtatc ggcgtcttgc
     1981 agtaagtcct gggcaatgag cagatcgatt tgcgccaggc cggcggtgct gtcgggcagg
     2041 gcggccacgc gctcgcgcag gcgcgcgatt tgttcgcgcc gcaccttgcg cgcttgcgcg
     2101 gtatccttcg gcttggaaat gccgaaccgc gcgggcgtga tctcccactc attgtggcgc
     2161 gaccaggtgt cggccagtgc gaccagggag acaaccaggc ggtcgttctc acgccgcgcg
     2221 gcaaagcctt cggccgcggc gtccagtagc tccagcgccg ctccggtctc tgccagcgcg
     2281 ctgcgcgccg cacccagtgc caagttgcat tccggccgtt cggccaattc cgggtgttca
     2341 cggtaaagct gcccgatgtc gccgattccc atctggcgat cagcgcggct ggggctggca
     2401 agccaagtca tcgcccgcag cgccccagc
//
LOCUS       DAOSYS010000378         2397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_33965_length_2397_cov_5.712212, whole genome shotgun sequence.
ACCESSION   DAOSYS010000378 DAOSYS010000000
VERSION     DAOSYS010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33965_length_2397_cov_5.712212"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLU99_15505"
     CDS             <1..449
                     /locus_tag="PLU99_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18498.1"
                     /translation="ASPKFYVPGVDGAPGDYITDVKPWMAVVILSRELAVSGIRAHAE
                     AGGEKFAALWAGKLKMFVQSVTALVILAEIGWNLKGVRPVSTALVWLTVIITVLSIIS
                     YLRRAHSFLLSSAALGATSATPPKVETPPSQPELPSSTNRSPGAAV"
     gene            458..1015
                     /locus_tag="PLU99_15510"
     CDS             458..1015
                     /locus_tag="PLU99_15510"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOO18499.1"
                     /translation="MPPRLKTAELIKTACITVLGSGYLWPPGTWGSAVGALAFVVIWW
                     LNGLAAGPQWTVDLIVVAGVLVSSVMSVIWGEWAIQRFGRADPHQFVLDEFAGQWVAL
                     LAVPLALAATPAALAAVVFGQFFFFRIADVIKPPPARQLEKLPAGWGILLDDLMAGVY
                     ANVAGQLLWRATPLASWLTQQTATA"
     gene            1037..2167
                     /locus_tag="PLU99_15515"
     CDS             1037..2167
                     /locus_tag="PLU99_15515"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOO18500.1"
                     /translation="MDALNTVVLGIIEGLTEFLPVSSTGHMVIAMPLLGVQADRPPWN
                     TLLWVSQFAAILAVILYFWRDLWTRTFNPRSWSWRQHILVKLFFAMVPTVVLALLFKK
                     RLDPLEQMPLAVAAALIVGAGLIELIDRRYRREGSQNLEDVTLSQAFFIGLIQCVSMV
                     PGVSRAAATIMGGMALGLTPRVATEFSFYLAIPTMLGAALKTLHDHRDELTGNNAGLI
                     VLGSAVSFVVALLVVAAFLEYVKRYRFTPFAVYRVVLGIVVIGLYMNGGFRVRLPQER
                     PQVLPEQAARYAPLAANGAIFVQNSHMTGAASSGESSTSSTPPNPGMLLPESLSAASR
                     FSSDSHKSPSTDRPAISRPKTSSVKSAAIPPGKRSVPEPYRP"
BASE COUNT          396 a          823 c          717 g          461 t
ORIGIN      
        1 ttgccagccc caagttttac gtccctggcg tggacggcgc ccccggcgat tacatcaccg
       61 acgtcaaacc ctggatggcc gtcgtcatcc tgtcgcgtga gctggccgtt tccggcatcc
      121 gcgcccacgc cgaagccggc ggcgagaagt tcgccgcgct ctgggccggc aagctgaaga
      181 tgttcgtcca atcggtaacc gcactggtga tcttggccga gataggctgg aatctgaaag
      241 gtgtgcggcc ggtgagcacc gctttggtct ggctgacggt gatcattacc gtgctctcga
      301 ttatcagcta tctgcgccgc gcacacagtt tccttctctc cagcgccgcg ctgggtgcaa
      361 ccagcgcgac gccgccgaaa gttgaaaccc cgccttcgca gcctgagttg ccgtcttcca
      421 ctaatcgctc gcccggagcc gcagtttgag gaccacggtg ccaccacggc ttaagactgc
      481 ggagcttatc aagaccgcgt gcatcacggt gctcggcagc ggctatctct ggccgccggg
      541 cacgtggggc tcggccgtgg gcgcgttggc attcgtcgtc atctggtggc tgaacggcct
      601 ggccgcgggc ccacagtgga cagtagacct gatcgtcgta gcgggcgtgc tcgtgagctc
      661 ggtcatgtcc gtaatctggg gcgaatgggc gattcagcgc ttcggccgcg ccgacccaca
      721 ccagttcgtc ctcgacgaat tcgccggcca gtgggtggcc ctgctcgctg tgccgctggc
      781 actcgccgcc acgcccgccg ccctggccgc agtcgtgttc ggccagttct tttttttccg
      841 cattgccgac gtcataaagc cgccgccggc tcgccaactc gagaagctgc cggccggctg
      901 gggcatattg ctggacgacc tcatggccgg cgtttacgcc aacgtcgccg gacaactgtt
      961 gtggcgggcg acgccgcttg catcctggtt aacgcagcaa accgcgaccg cgtaactcca
     1021 ctgcgaaggg caataaatgg atgcgttgaa cacggtcgtc ctgggcatca ttgagggcct
     1081 gactgagttc ctgcccgtct ccagcaccgg gcacatggtg atcgccatgc cgctgctggg
     1141 cgtccaagcc gaccgcccgc cctggaacac gctcctgtgg gtttcgcaat ttgcggctat
     1201 tctggccgtt atcctctact tctggcgcga cctctggacc cgcacgttca acccccgttc
     1261 ctggtcgtgg cgccaacaca tactcgtcaa gctgttcttc gccatggtgc cgacggtcgt
     1321 gctggcgctg ctgttcaaga agcggcttga tccgcttgaa caaatgccgc tggccgtcgc
     1381 cgccgcactg atcgtcggcg ccggactcat tgagctgatt gaccgccgct accgccgcga
     1441 gggctcgcag aacctcgaag acgtcacgct ttcgcaggcc ttcttcatcg gcctgattca
     1501 atgcgtcagc atggttcccg gcgtctcgcg ggccgccgcc accatcatgg ggggcatggc
     1561 gctcggcctg acgccgcgcg tggcgaccga gttctccttc tacctggcga ttcccacgat
     1621 gttgggcgcg gcgctcaaga ccctgcacga ccaccgcgat gaactgaccg gcaacaacgc
     1681 cggcctgatt gtgctcggct cggccgtctc attcgtggtc gccctgctgg tcgtggcggc
     1741 ctttctggaa tacgtgaaac gctaccgctt tacccccttt gccgtgtatc gcgtcgtgct
     1801 gggcatcgtc gtaatcgggt tgtacatgaa cggcggcttc cgtgtgcgac tgccgcagga
     1861 gcgcccgcag gtcttgcccg agcaggccgc ccgctacgcg ccgctggccg ccaacggagc
     1921 gatcttcgtc cagaacagcc acatgaccgg cgcggccagc agcggcgaat cgagcacatc
     1981 cagcacgccg ccgaatccgg gaatgctgtt acccgaatct ttgagcgcgg cgtcacgttt
     2041 cagtagtgac tcgcataagt cgcccagcac cgacagaccg gccatcagca gaccgaaaac
     2101 cagcagcgtg aaaagcgccg ctatcccgcc cggcaagcgc agcgtgccgg agccgtacag
     2161 gccatagcca acgccgatcg cgaacgcggt cgcaacgatg attcctccgg cgaaaccctc
     2221 ccaggtcttc ttcgggctga gccattcgat cagcttgtgc cggcccgttg ccatcccggt
     2281 gaaatacgcg ccgatgtccg tcaccttcac gacgaagacg gagtacagca gcagaatcgc
     2341 cccgctttcg ccgcccacgt ccatccgcag ccgcgtaaag aattgagcca gcccccc
//
LOCUS       DAOSYS010000379         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_34880_length_2347_cov_4.534031, whole genome shotgun sequence.
ACCESSION   DAOSYS010000379 DAOSYS010000000
VERSION     DAOSYS010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34880_length_2347_cov_4.534031"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /gene="manB"
                     /locus_tag="PLU99_15520"
     CDS             complement(<1..1146)
                     /gene="manB"
                     /locus_tag="PLU99_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOO18501.1"
                     /translation="MLQTPDQYARHCPGEEKVPISDAICFGRQRANFRKCSNCEFNEE
                     RAGPLLGTLAGGAGGQAPSREPPRAGVMEKVFKAYDVRATVPDPLNEDVAWRIGNATA
                     QFLRTALSGFDRSDPKMDSLILGRDMRRSSPALAKALADGVLAVGTNVIDIGLVDTPQ
                     VTFATNVLPCCGGVQTTASHNAANYNGFKILGQKGKPIGAETGLQEIQRIAAAISRYE
                     APVTAKVQRLELAREYREYLLKFLQPLARPLKVVVDASNGMAGRWFPIVFDGTPNLQV
                     IPLNFEHNGEFAHPPNPLVAANLEDLRTAVREHGADFGACLDGDADRCIFVDEYAEIV
                     RCDLLTALLAGEYLRQKPGATIIYDVRCSRIVGEHVSKLGGVARRERV"
     gene            1489..>2347
                     /locus_tag="PLU99_15525"
     CDS             1489..>2347
                     /locus_tag="PLU99_15525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOO18502.1"
                     /translation="MQSTALFSEVESPSSPLAARDWCRPAFVVVLLFAAAIRLVALPA
                     APPGLNQDEAANAWNAWCLLQTGRDQAGAPWPIFWTHALGENRSTLYLYALLPFQAIG
                     GLNVWTTRLPAAVAGILTVALVYWIAARLFDRTTAIIAAVFAALAPWYFDLSRWGHEA
                     SLGPLLTAAAFAALLWAGLLETDRDRDQQPGPDKLVQTTARGPNWWRAALAGLICGAA
                     CYGYPAVRLYLPLLIVTALIVDIASTRRLLRSRSGRIAAAMFCLGFALTFGPLLFQHI
                     VHPEVIGRRA"
BASE COUNT          368 a          843 c          698 g          438 t
ORIGIN      
        1 cacccgctcg cgtcgcgcta cgccccccag cttgctcacg tgctcgccca cgatccggct
       61 gcaacgaacg tcgtaaatta tggtcgcccc gggcttctgc cgcaggtact cccccgccag
      121 cagtgcggtg agcaggtcgc agcggacgat ctcggcgtat tcgtcgacga agatgcagcg
      181 gtccgcgtcg ccgtcgagac atgctccgaa gtccgccccg tgctcgcgca ccgccgtccg
      241 caagtcctcg aggttcgcgg ccaccagcgg gttgggcggg tgtgcgaatt cgccgttgtg
      301 ctcgaaattc agcgggatga cctgcaaatt cggcgtgccg tcgaagacga ttgggaacca
      361 gcggccggcc atgccgttcg acgcgtcaac cacgaccttc aacggccgcg ccagcggctg
      421 caggaacttc aacaaatact cgcgatattc gcgcgctagc tccagtcgct gcaccttcgc
      481 cgttaccggc gcctcgtagc gcgaaatggc cgccgcgatc cgttgaatct cctgcagtcc
      541 cgtctcagcc ccgataggct tgcccttttg ccccaaaatc ttgaaaccgt tgtagttggc
      601 cgcgttgtgg ctggcggtcg tttgcacccc gccgcaacaa ggcagaacgt tcgtcgcgaa
      661 agtaacctgc ggcgtgtcga ccagcccgat gtcaatgacg ttggtgccga ccgccagcac
      721 gccgtccgcc agcgccttgg ccaacgccgg gctggagcgg cgcatgtcgc ggccgaggat
      781 gagcgagtcc attttggggt cactgcggtc gaagccggag agcgcggtgc ggagaaattg
      841 ggccgtggcg ttgccgatgc gccaggcgac gtcctcgttg agcgggtcgg gcaccgtcgc
      901 ccgcacgtcg tacgccttaa aaaccttttc cataacgccc gctcgcggcg gctcgcgcga
      961 gggcgcctgc ccgcccgccc cgccggccag cgtcccgagc aacgggccgg ctctttcctc
     1021 attgaactcg cagttggagc acttgcggaa attcgcccgc tgccggccga agcagatggc
     1081 gtctgagatc gggaccttct cctcgcccgg gcaatggcgg gcgtactggt caggcgtctg
     1141 aagcacgtaa acaactcccg gtgcgacccg cggggcagct ccacgccgac aacccgcagg
     1201 catttccagc acgcagcccg cggacgaaat cgcgccgccg gccgcgcggg caaccgacac
     1261 gttctccaca tgctatcgca cgcggccacg ttcccgcaat cacccgggac gtagcgcggg
     1321 tagcaccgca ggccacgcag ccgcacgccc agattgtgat aaggtgataa ggtgataaag
     1381 ggtggtctgc ggaatcactt tatcactcta tcaccttatc accgttcact ttatcacttt
     1441 gtccgcatca ctctgtccgc gagcccccct gccgcttagg atgtacgcgt gcagagcaca
     1501 gccctgtttt ccgaggtcga atccccttca tcgccgctgg ccgcgcgtga ttggtgccgc
     1561 ccggctttcg tcgttgtgct gctgttcgcg gctgccattc gcctggtggc cctccccgcg
     1621 gccccgcccg gcctcaacca ggacgaagcc gccaacgcct ggaatgcctg gtgcttactg
     1681 caaaccggcc gcgatcaggc cggcgccccc tggccgatct tctggactca cgcactgggc
     1741 gaaaaccgct cgacgctata cctgtacgcg ctactccctt tccaagccat cggcggtttg
     1801 aacgtctgga ccacgcgcct cccggccgcc gtggccggca tcctcaccgt cgcgctcgtg
     1861 tactggatcg ccgcccgcct gttcgatcgc acgaccgcga ttatcgccgc cgtttttgcg
     1921 gcgctggcgc cgtggtactt cgacctgagc cgttggggcc acgaagcctc cctcggtccg
     1981 ctcctaacgg ccgcagcctt cgcggccctg ctctgggccg gactcctgga aactgaccgc
     2041 gatcgcgacc aacagcccgg cccggacaag cttgttcaga ctacagcgcg cggcccaaac
     2101 tggtggcgtg ccgcgctcgc gggtctgatc tgcggagccg catgctacgg ctaccccgcc
     2161 gtgcgcctct acctgccgct cctcatcgtc acggcactga tcgtcgacat cgcttcaacg
     2221 cgccgattgc tgagatcgcg ctccggccgc atcgccgcag cgatgttttg cctcggtttt
     2281 gcgcttacgt ttggaccgct ccttttccag cacattgttc atcccgaggt aatcggtcgg
     2341 cgtgccg
//
LOCUS       DAOSYS010000380         2314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_35495_length_2314_cov_5.391324, whole genome shotgun sequence.
ACCESSION   DAOSYS010000380 DAOSYS010000000
VERSION     DAOSYS010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35495_length_2314_cov_5.391324"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLU99_15530"
     CDS             complement(<1..388)
                     /locus_tag="PLU99_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18503.1"
                     /translation="MLEAYTGSKPGRRTTLLIMVALVLAGALALAWSQVRATRTLGPP
                     IAIPGTPLLVRLPAQWKQDPHKPTAFVLPTRGDSRQQTADFERRISFQYERTAGFRPP
                     LTSLKALAGRRTAEPARIGPFAAVQVR"
     gene            complement(538..2028)
                     /locus_tag="PLU99_15535"
     CDS             complement(538..2028)
                     /locus_tag="PLU99_15535"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOO18504.1"
                     /translation="MSVLRNGRWLRLPLLVLVLSWPLVAQAGSPDDQNRISQLEALLN
                     AQQQRIASLEQQVATAQASDDERRLGLMRQQIREILSEQEFRESLMPTVAQAGYDNGF
                     FIRSSDDKFLMKLFGRTQFRWTYYNQEHRNHYLLPRYERDDRTGFDLRRIRFGLNGHV
                     YSRDLTYLMEFQWDSASAYQGRLQYIWTNYRFVDEFQMKFGLFQAAGPRAGFNSSSNM
                     QFPEYPTNEAVFGTGTGIGVRFWGQAFNKRLEYFLDVVNSLSTTTNQVIGPDPAQLDG
                     NPAILFRTVWHALGENPPNDFASWGDLEYHECPALDIGFHYVFDDNYGDNPSRRIIFN
                     RRSLLPGGFGVTSSQGIQTNQFGLDSAFKLRGFSIAGEYDVMVLDPRRAGRTPFTPLW
                     LLTGEDATTTVNGGYVQAGYFLPIPGLERKLEVVARVGGLQTNAGGSEGTWYYQGGVN
                     YYIEGNKVKLQADVEKISEVPISSNSWLANPNDDALIFRVQLQVAF"
     gene            complement(2110..>2314)
                     /locus_tag="PLU99_15540"
     CDS             complement(2110..>2314)
                     /locus_tag="PLU99_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18505.1"
                     /translation="RCKCDADHMAALRDWPPRLRLTEIRRLLFKISPKLVGDALVSRS
                     RIIMFSIQVLDKYARKRAGAVLT"
BASE COUNT          465 a          684 c          715 g          450 t
ORIGIN      
        1 gccgcacctg taccgcagca aacggtccaa tacgggccgg ttccgccgtg cgtctgccgg
       61 ccaatgcttt tagcgatgtc agtgggggac ggaacccggc ggttcgctcg tactgaaaac
      121 tgatccgccg ctcaaaatca gcggtttgct gtcgtgaatc gccgcgcgtc ggcagaacga
      181 aggcagtcgg cttgtgcgga tcctgtttcc attgcgctgg caatcgcacc agcaacgggg
      241 tgcccgggat ggcgatcggc gggcccagcg tgcgcgtcgc gcgtacctgc gaccatgcca
      301 aggccagcgc ccctgccagg accagggcaa ccatgatcag cagcgtggtt cggcgtccgg
      361 gttttgatcc agtataagct tcgagcatag acgggcatga tacgagaagg gcacaggggt
      421 ttaaatgatg gaccgcgcgg tactatcgcc gcggcggaaa aaaacggaaa tcgaggccgg
      481 ctcgcggcca gcctcgattt ccgaacttgt ttcactcttc agcggtcgcc ggcgccctta
      541 aaacgccacc tgtaattgca cgcggaagat aagggcgtcg tcgttcgggt ttgccaacca
      601 gctattcgag gagatcggca cttccgaaat cttctcgacg tccgcctgca acttgacctt
      661 gttcccctcg atgtaatagt tcacgccgcc ctggtaatac caggtgcctt cgctgccgcc
      721 ggcattggtc tgcaagccgc cgacacgagc gacgacttca agcttgcgct ccagccccgg
      781 gatgggcagg aagtaaccgg cctgcacgta gccgccgttc accgtggtgg tcgcatcttc
      841 cccggtcagc agccatagcg gcgtgaaggg ggtgcggccg gcacggcgcg ggtcgagaac
      901 cattacgtcg tactcgccgg cgattgagaa cccgcgcagc ttgaacgcgc tgtccagccc
      961 gaattggttg gtctggatgc cctgtgagct ggtcacaccg aagccgccgg gaagcagact
     1021 tctgcggttg aaaataattc gccgggaggg gttatcgccg tagttgtcgt cgaagacgta
     1081 gtggaaaccg atatcgagcg ccgggcactc gtgatactca aggtcgcccc aactggcgaa
     1141 gtcattgggc ggattctcac ccaacgcatg ccagacggtg cggaacagga tcgccgggtt
     1201 gccgtcgagc tgcgccgggt caggcccgat tacctggttg gtcgtagtgg acagcgagtt
     1261 gactacgtcc aggaagtatt ccaggcgctt gttgaacgcc tggccccaga agcggacacc
     1321 gataccggtg ccggtgccga agaccgcctc gttcgtggga tattcaggga actgcatgtt
     1381 ggagctggag ttgaaccccg cccgcggtcc cgccgcctgg aagaggccga acttcatctg
     1441 gaactcgtcg acgaagcggt aattcgtcca gatgtactgc agccggccct gatacgcgct
     1501 cgcggaatcc cactgaaact ccatcaagta cgtgagatcg cgggagtaga cgtggccgtt
     1561 aaggccgaag cggatgcgcc gcaggtcgaa gccggtgcgg tcgtcacgct cgtagcgcgg
     1621 caaaaggtag tggttgcggt gctcttgatt gtagtaagtc cagcggaact gcgtacgacc
     1681 gaacaacttc atcagaaact tgtcgtcgct ggagcggatg aagaaaccat tgtcgtagcc
     1741 ggcctgagcg accgtgggca tcaggctttc gcgaaactcc tgctccgaca ggatttcgcg
     1801 gatttgttgg cgcatcaggc ccaggcgccg ttcgtcgtcg ctggcctgtg cagtggccac
     1861 ctgttgctca aggcttgcga tgcgctgctg ctgagcgttg agcaaggctt cgagctgcga
     1921 aatccgattc tgatcgtcgg gactgccagc ctgcgcaacc agcggccagc tcaagacaag
     1981 aaccaacagg ggtagccgca gccagcggcc gtttcttaaa acggacatca acatcctcct
     2041 gagttcgatg ccgaccggcg cgttcggaaa accaacctgg ccccgtttcg aaactccgtt
     2101 tcgaccgggc tatgttagga ccgcgccagc gcgcttgcgc gcatacttat ccaatacctg
     2161 aatggaaaac ataatgattc tcgagcggct gacaagggca tctccgacca gttttggtga
     2221 aatcttgaac aataggcgac ggatttccgt cagccgcagc cgtggcggcc agtctctcag
     2281 cgccgccatg tgatcggcat cacatttaca tcgg
//
LOCUS       DAOSYS010000381         2312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_35527_length_2312_cov_5.869296, whole genome shotgun sequence.
ACCESSION   DAOSYS010000381 DAOSYS010000000
VERSION     DAOSYS010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35527_length_2312_cov_5.869296"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="PLU99_15545"
     CDS             complement(<1..1250)
                     /locus_tag="PLU99_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOO18506.1"
                     /translation="MGLVLLGLTLYISTLAYTLRDYSRAEVSRELGNSGRQRWLDWLD
                     AHEEELQGSTSFGRVVCNLGLLVCITLWYLRGVEGGVGVGALIVPAVISLLLLLVFSV
                     GIPQGLSEYVGEAVLARSLPVLAFMWVVFWPVAQVVRAIKFFIRRLLGRSEETSAEET
                     ERMEQEILEAVSEGEASGAVDEEQKEIIESVFELNETDVAAIMTPRTDINAVRADCTY
                     EQARDAVLRGGHSRIPVYENSIDHIIGVLYAKDLLRLQPGEPFDARRLMRAAQYVPET
                     KSISELLKEFRLSKVQIAIVLDEYGGTAGLVSLEDILEELVGEIVDEYDQHQPPAIRH
                     IDQDTLEVDARVHVDEINQELNVTLPENGDYETIGGFVFSKLGKIPAVGEEFAHGNVL
                     FRVTAAESRKINRVHIHVQRAAAE"
     gene            complement(1347..>2312)
                     /locus_tag="PLU99_15550"
     CDS             complement(1347..>2312)
                     /locus_tag="PLU99_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOO18507.1"
                     /translation="SARPAEWEARLFLHGSPWVVRRCCELLVKAGFSQRETPCAELWP
                     ARDFIESEAYALLPQVQTLEGATWLLGQAKRLRRAIEELLEQDWREGRQACRDLAERL
                     CVFDWYSRPARVALVGPTNAGKSTLANALAQQEVSLTSAQPGTTRDWVEVPVAVRGFP
                     MVWLDTAGLVERSQGLEAAALEKTRQCVSGADAVVLVLDGTSASVPDLRAVGLNAENA
                     PTVVALNKFDRAEFRQDLHDSFPPGWCDRIVAISAAEGTNLAALEDALVESLGRHDSF
                     LKLPGAFTARQAAWLKAAAEALDECFYRTALEAVFSPNDATTLHP"
BASE COUNT          438 a          818 c          638 g          418 t
ORIGIN      
        1 tcctccgccg ccgcacgttg cacgtgaata tgaacgcgat tgatcttccg cgactcggcc
       61 gccgttacgc ggaacaacac gttcccgtgt gcgaattctt cgcccaccgc cggaatcttg
      121 cccagcttgg agaagacaaa tccgccgatc gtctcgtagt ccccgttctc cggtagcgtg
      181 acgttcagtt cctggttgat ctcgtccacg tgtacgcggg cgtctacttc cagcgtgtcc
      241 tggtcgatgt ggcgaatggc cggcggctgg tgctggtcgt attcgtcgac gatttcgccg
      301 accagctcct cgaggatgtc ctccaggcta accaggccgg ccgtcccgcc gtactcatcc
      361 agcacgatcg cgatctgaac cttgcttagc ctaaattcct tcaacaattc cgaaatcgac
      421 ttcgtttccg gcacgtactg ggcggcccgc atcaggcgcc gcgcatcgaa cggctcaccc
      481 ggctgcagtc gcagcaggtc tttcgcgtac agcacgccga tgatgtggtc gatcgaattc
      541 tcgtaaacgg gaatgcgtga atgcccgccg cgcagaaccg cgtcgcgcgc ctgctcgtag
      601 gtgcaatccg cccggacggc gttgatgtcc gtccgcggcg tcatgatcgc cgcgacgtcc
      661 gtttcgttga gctcgaagac ggactcgatg atttcctttt gctcttcatc aaccgcgccg
      721 gaggcctcac cctcggaaac cgcctccagg atctcctgct ccatgcgctc ggtttcctcg
      781 gccgaagtct cttcggaccg ccccagcagg cgccggatga agaacttgat ggcgcggacc
      841 acctgtgcca ccggccagaa tacgacccac atgaacgcca gcacgggcag actgcgggcc
      901 aggaccgcct cgccgacata ttccgaaagg ccctgcggga tgccaacgct gaagaccagc
      961 agcaatagaa ggctgattac ggccggcacg ataagcgcgc cgacacccac gccaccctcg
     1021 acgccacgga gataccataa agtgatgcag acgagcagcc ccaggttgca tacgacccgc
     1081 ccaaagctcg tcgagccctg taattcctcc tcgtgcgcgt caagccaatc cagccaacgc
     1141 tgccggccgc tgttacccag ctcccggctg acctcggctc gcgagtaatc ccgcagggtg
     1201 tacgccagcg tggagatgta cagggttagg cccagaagga ccagacccac ccctagttcc
     1261 aaatacctac tccagacagg gccggaaagc cgggccgatc catcggccaa cggcattcca
     1321 ccggcatgac tatatcggct attttattac gggtgcaagg ttgtcgcatc attcggggag
     1381 aaaacggcct ccagcgcggt cctataaaag cattcgtcca gtgcctcggc ggcagctttc
     1441 agccaagctg cctggcgggc cgtgaacgct cccggcaact tgagaaaact gtcatgccgg
     1501 ccgaggctct ccaccagcgc atcctccagc gcagccaggt tcgtaccctc agcggctgag
     1561 atcgccacga tcctgtcaca ccagcctggg gggaaagagt catgcaggtc ctgcctaaac
     1621 tctgcgcggt caaacttgtt cagcgccacg acggtcggcg cgttctccgc gttaaggccg
     1681 accgcccgta gatccggcac gctcgcactg gtgccgtcca gcacaagcac caccgcatcc
     1741 gcgccgctga cgcattgccg tgtcttttca agggccgctg cttccagccc ctgcgaccgt
     1801 tccaccagcc cggccgtatc cagccaaacc atcggaaaac cacgcaccgc gaccggaacc
     1861 tcgacccaat cgcgcgtcgt gcccggctgg gccgatgtca ggctgacctc ctgctgagcc
     1921 agcgcgttgg cgagggtgct ttttcctgcg ttagtcggcc ccaccagcgc cactcgcgcc
     1981 ggccgcgaat accaatcgaa gacgcacaga cgctcggcca agtctcggca cgcctgccgg
     2041 ccttcgcgcc agtcctgttc taacaactcc tctatcgctc tccgcagccg cttcgcttgt
     2101 ccaagcagcc aggttgcccc ttcaagcgtc tgcacctgcg gcaggagcgc gtaagcctcc
     2161 gattcgatga aatcccgagc cggccatagc tccgcgcagg gcgtctcccg ttgcgaaaag
     2221 ccggctttca ccagcagctc gcaacagcgc cgcacgaccc agggactgcc gtgcagaaag
     2281 aggcgcgctt cccactccgc gggcctggca ga
//
LOCUS       DAOSYS010000382         2309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_35582_length_2309_cov_5.760870, whole genome shotgun sequence.
ACCESSION   DAOSYS010000382 DAOSYS010000000
VERSION     DAOSYS010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35582_length_2309_cov_5.760870"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLU99_15555"
     CDS             complement(<1..390)
                     /locus_tag="PLU99_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOO18508.1"
                     /translation="MKTGLFYGSTTGNTKKVAEMIRAALGDAITIFKNIRDTTPEEFA
                     SCDKLILGISTWEEGALQEHWKVFFPALDGMDLRGKTVALFGLGDQGGYSGRFQDAMG
                     TLYRKVCERGATVVGAWPRRGYRAATST"
     gene            complement(387..1052)
                     /locus_tag="PLU99_15560"
     CDS             complement(387..1052)
                     /locus_tag="PLU99_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOO18509.1"
                     /translation="MLELAEQMCAIGRRAYARQLVAGTEGNFSCRLGPETVLCTPTAM
                     CKGLLRPVDLCIVDLSGRQVSGIRPRSSEILMHLGLYRAAPHIRAIVHTHPPFATTWS
                     LAGAVPLAGLLPEGDIFLGEVPVVPYRTPGTADMAAPLLPLLEEHDVAILQNHGAVTW
                     GADLETAYVLTETLEAVCRVAYQARQIGNEGRIPPQERQVLAKLRAELRARGKSRILE
                     RPG"
     gene            complement(1120..1854)
                     /gene="ispD"
                     /locus_tag="PLU99_15565"
     CDS             complement(1120..1854)
                     /gene="ispD"
                     /locus_tag="PLU99_15565"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOO18510.1"
                     /translation="MSKVAVIIAAAGAGKRFGGDVKKPFAQIDNRPIFIRSIELFINR
                     PDVCQNILAVSAGDYDVVREKYAANIMFMGIKLVKGGQERYQSIQAALKEVDPQADLV
                     CVHDAVRPCVMETWIDSVFAEAGKSGAAILAAPITGTLKRAAESGFIDETVPRAGLFE
                     AQTPQVFSRELLLRAYKEMPANFKPTDDAQVVERLGHAVRIVQSDHRNLKITTPGDLE
                     LAAAVLKHMSKRPKGPAMGAFEEAQW"
     gene            2057..>2309
                     /locus_tag="PLU99_15570"
     CDS             2057..>2309
                     /locus_tag="PLU99_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18511.1"
                     /translation="MSKLQNKRALAAGASLLLLLSGCENFSIRPPSTPPPEPKPPPMA
                     PLTLDPVLAGSIGAATFLSEVDPVEVRGFGLVIGLGKNGS"
BASE COUNT          414 a          758 c          707 g          430 t
ORIGIN      
        1 cgtcgaggtg gccgcccggt agccacggcg cggccaggcg cccaccaccg tcgcgccgcg
       61 ctcgcagact ttgcgataca aggttcccat cgcgtcctgg aaccggcccg agtagccgcc
      121 ctgatcaccc agcccgaata gcgccactgt tttgccgcgc aagtccattc catccagcgc
      181 ggggaagaac actttccagt gctcctggag cgcgccctct tcccaggtcg agatgccgag
      241 aatcagcttg tcgcaactgg cgaattcttc aggcgtggtg tcgcggatgt tcttgaagat
      301 tgtgatcgcg tcgcccaacg cggcgcggat catctccgcc acctttttcg tgttgcccgt
      361 cgtgctaccg tagaagagtc ctgttttcat ccggggcgct ccagaattcg cgatttgccg
      421 cgcgcccgca attcggcgcg gagcttggcc aagacctgcc gctcctgtgg cggaatccgg
      481 ccttcgtttc cgatttggcg cgcttgatag gcgactcgac agaccgcctc gagcgtctcg
      541 gtcagcacgt aggccgtctc caaatccgcg ccccaggtaa ccgcgccgtg gttctgaaga
      601 atcgccacat cgtgctcttc caacaacggc aggagcggcg ccgccatatc ggccgtaccc
      661 ggcgtgcgat agggcacgac cggcacctca cccagaaaga tgtctccttc tggcaagagt
      721 ccggccaaag gcactgcccc ggcgagcgac caggtcgtcg caaatggcgg gtgcgtgtgc
      781 actatcgcgc ggatatgcgg agcggcgcga tacaggccca ggtgcatcag tatctcgcta
      841 ctgcgcgggc gaatgcccga cacttggcgg ccgctgaggt ccacgatgca caggtcgacg
      901 ggccgcagca ggcccttgca catcgcggtc ggcgtacaga gtacggtttc ggggccgagg
      961 cggcaggaga agttgccttc ggttccggcc acgagttggc gggcataagc ccggcggccg
     1021 atggcgcaca tctgttcggc aagttcgagc atgtcaggcg atggtatcgc cggcgcggag
     1081 cagtaacaac ggtaaagaag tgaccagcgg ccggttacct taccactgcg cttcctcgaa
     1141 cgcccccatg gccggtcctt tcgggcgctt gctcatatgc ttcagcaccg ccgcggccaa
     1201 ttcgaggtcg cccggagtgg tgatcttcag atttcggtga tcgctttgca cgatccgcac
     1261 ggcgtgcccg agccgttcga ccacttgcgc gtcgtcagtc ggcttgaaat tcgccggcat
     1321 ctccttgtac gcccgcagca gcagctcgcg cgaaaatacc tgcggcgtct gggcctcgaa
     1381 tagccccgca cgcggcaccg tctcgtcgat aaagcccgat tcggccgccc gcttcagtgt
     1441 tcccgtgatg ggcgctgcca gaatcgccgc cccactcttg cccgcctccg cgaagacaga
     1501 gtcaatccaa gtttccatca cgcacgggcg caccgcgtcg tgcacacaca ccaggtcggc
     1561 ctgcggatcg acctctttaa gcgccgcctg aattgactga tagcgttcct gcccgccctt
     1621 caccagcttg atgcccatga acatgatgtt cgccgcgtat ttctcgcgca ccacgtcgta
     1681 atcaccggcc gaaacggcca agatgttctg gcagacatcc ggccggttga taaaaagctc
     1741 gattgagcgg atgaaaatcg ggcggttatc gatttgggcg aacggctttt tgacatcgcc
     1801 gccaaagcgt ttgccggcgc cggccgccgc gatgatgact gcgactttgg acattaaggc
     1861 gctcctttta atccgcacta accgaggata acatgcgggt ccgaaagggc gaggtcaagg
     1921 ctggcttttg tgccttgagg tccggcgata ctgcgtcgga cggccggcca gcgggcgtga
     1981 ggaaggacgt tcgcatcagc aaccaacctg gcggggaagt gccgatgacc cggccacgac
     2041 cgcacgagat catcgcatga gcaaactcca aaacaagcgg gcactggcag ccggggcaag
     2101 cctgctgttg ctgctgtcag gctgtgagaa cttctcgatc agaccgccat ccacgccgcc
     2161 gccggagccc aagccgcctc ccatggcccc gctgactctc gacccggtgc tggcgggcag
     2221 catcggcgcg gcgaccttcc tctcggaagt cgatccggtg gaggtgcgcg gcttcggttt
     2281 ggtcatcggg ctgggcaaga acggcagcg
//
LOCUS       DAOSYS010000383         2294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_35858_length_2294_cov_4.373828, whole genome shotgun sequence.
ACCESSION   DAOSYS010000383 DAOSYS010000000
VERSION     DAOSYS010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35858_length_2294_cov_4.373828"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PLU99_15575"
     CDS             complement(<1..1493)
                     /locus_tag="PLU99_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOO18512.1"
                     /translation="MSDFLIQFGSGLSRETVADVMRGREYMGDLAVEHFEHGWGSISI
                     QQSRCFGYRPYVSNGHTYFALGRPRIIGVTHELDGPQGFTKALAGWMEVLEAERRYEN
                     LTGIFAIGRCGPEGVVLLTDRMGAYSVYAARDHKGQVRAIGSLPELVARAAGRESDFD
                     RTSLAEIMLRYAPMFPYTTRRGMHELTPGCFHEFVTRAGSVEHVEQCLWTPNEPDRYP
                     SLASLREQLVAALRTAGAELTRGAQRVAVTLSGGFDSRTVLAVIPPVKRAAAITYIDH
                     GNYEFGVANLVARCAAVPHLPIQRSPDFYATIVPRELAFFGAEHQAQHAHGFALADIG
                     MCKAYDLVLSGIYSDALLKGYFSPDSTRGYLLGRAGVPRAVRERCCKPRYDWTAPGRP
                     TVMHAFRPAIRDAIRERRAARLAQLEELRPDSAREWLTFYPFSRAMAGNYYASNVRLF
                     PADELFLYRDIVEVGRTAPLLPKLMDRLAVPAFSVVCGPLADIENANT"
     gene            complement(1510..>2294)
                     /locus_tag="PLU99_15580"
     CDS             complement(1510..>2294)
                     /locus_tag="PLU99_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOO18513.1"
                     /translation="GRLWGPLPLGLYDRSHALVLMPMLQINPPLAAVALPALSRAAET
                     PERYARAYVSILRQLALVTAVLVPILIVNASTVIRVVLGPSWAAAGPLFGVLGFAALL
                     LPIWNAIGWVFISQNRTRELLNWHALDLVFKVASVFAGVPWGVMGIAVAVSVRYYVQL
                     PVLFWLAGRRGAVRTGELYRAVSLPACVAVSSLAGLTLISRVLADFPDVMRLLLAGLA
                     ALAISGCVLWLTREGRRTLGEIRELGRALLRRPQAAISASSV"
BASE COUNT          449 a          740 c          761 g          344 t
ORIGIN      
        1 gtgttcgcgt tctcgatgtc agccagcggc ccacagacga ccgaaaaggc gggcacagcc
       61 agccggtcca tcagcttcgg tagaaggggc gccgtccgcc cgacttccac tatgtcgcga
      121 tacagaaaca gctcgtcggc cgggaacagc cgcacgttgc tggcgtagta gttgccggcc
      181 atagcgcggc tgaagggata gaatgtgagc cactcgcggg cgctatctgg ccgcagttcc
      241 tccaactggg cgaggcgtgc ggcgcgacgc tcgcggatcg cgtcccggat ggcggggcgg
      301 aaggcgtgca ttacggttgg gcgcccgggg gcggtccaat catagcgcgg cttgcagcag
      361 cgctcgcgca cggcgcgtgg tacgccggcc cggcccagca agtaaccgcg cgtggaatcg
      421 gggctgaaat agcccttcag cagcgcatcg gagtaaattc cgctcaaaac caggtcgtac
      481 gccttgcaca tgcctatgtc cgccagcgca aaaccgtggg cgtgctgggc ttggtgctcg
      541 gcgccgaaga acgcaagttc gcgcggcacg atggtggcgt aaaagtcggg gctgcgctgg
      601 attggcagat ggggtactgc ggcgcagcgg gccaccagat tcgccacgcc gaattcgtag
      661 ttcccgtgat cgatgtacgt gattgccgcg gcccgcttca cgggcggaat cacggccaga
      721 acggtacggg agtcaaagcc gccgctgagg gtgacggcga cccgctgggc gccgcgagtc
      781 agctcggccc cggcagtgcg caaggcggct accaattgtt cacgcaggct ggcgagcgac
      841 ggatagcggt cgggctcgtt cggcgtccac aagcactgct cgacgtgctc gacggatccg
      901 gcccgggtca cgaattcatg aaagcagccg ggcgtgagtt cgtgcattcc gcggcgcgtg
      961 gtatagggaa acatcggcgc gtagcgcagc atgatttcgg ccagcgacgt gcggtcgaaa
     1021 tcgctctcgc gtccggcagc gcgggccacc agctccggga ggctgccgat cgcccgcact
     1081 tgtcccttgt gatcacgggc cgcgtaaacg gagtacgcgc ccatgcgatc ggtcaggaga
     1141 actacacctt ccggaccaca acgtccgatg gcgaagatgc cggtcaggtt ttcgtagcgc
     1201 cgttcggcct cgaggacctc catccagcct gccagcgctt tcgtgaagcc ttgcgggccg
     1261 tccagctcgt gtgtcacgcc gatgatgcgc gggcggccga gcgcgaagta cgtgtgcccg
     1321 ttgctgacgt acggtcgata gccgaaacag cgcgattgct ggatgctgat gctgccccag
     1381 ccgtgctcaa agtgctcgac tgccaagtca cccatgtact cgcggccgcg catgacatcg
     1441 gcgaccgttt cacgacttaa gccggagccg aattggatta gaaagtcgct catggcattc
     1501 tcgcagaact cagacactgg aagccgaaat cgccgcctgc gggcggcgca gcagggcgcg
     1561 ccccaactcg cggatttcgc ccagcgtgcg gcgaccctcg cgagttagcc aaagaacaca
     1621 gccggaaatg gccagcgccg ccagtcccgc taatagcagg cgcatcacgt ccgggaagtc
     1681 cgccagcacc cggcttatca gcgtcaggcc ggccaggctg ctgaccgcca cgcacgccgg
     1741 taggctcacg gctcggtaca gctcgccggt ccgcaccgcg ccacgccgcc cggccagcca
     1801 aaacaggacc ggaagctgaa cgtaatagcg cactgacacg gccacggcga ttcccatcac
     1861 gccccagggc acgcctgcaa agaccgaggc cactttgaaa accaggtcga gcgcatgcca
     1921 attcagcagt tcacgcgtcc ggttctgcga gataaatacc cagccgatgg cgttccagat
     1981 cgggagcagg agcgcggcaa acccgagcac gccgaatagc gggccggccg ccgcccacga
     2041 cggcccgagc acgactcgaa ttaccgttga agcattcacg atcaggatcg ggaccagaac
     2101 agccgttacg agcgcaagct gccgaaggat agaaacatag gcgcgggcgt aacgctcggg
     2161 agtctccgcg gcccggctga gagctggaag cgccacggcg gccagcggcg ggttgatctg
     2221 cagcatcggc ataagcacca gggcgtgcga gcggtcataa agccccagcg gcagggggcc
     2281 ccagaggcgt ccaa
//
LOCUS       DAOSYS010000384         2264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_36475_length_2264_cov_3.842463, whole genome shotgun sequence.
ACCESSION   DAOSYS010000384 DAOSYS010000000
VERSION     DAOSYS010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36475_length_2264_cov_3.842463"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="PLU99_15585"
     CDS             <1..1103
                     /locus_tag="PLU99_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOO18514.1"
                     /translation="GQVNSLEGAGGIGGLLAVYDLNDTPGAEQTGDDLQYVYTYDGNG
                     NVVQVLDWAASSAVEAIKARYEYDPYGNVVAQGGDYAERNPFRFSTKYWDDETGLGYW
                     GYRYYSSRMGRWISRDPIFRSESPNLNAALHNDPLSWIDPDGRQEHWGGPVSEPPLPL
                     PPTSGPASQPAVDKSKACCVYREVSSSADSHGMGVHTNTCYKVHRCPPGATSPGQCCC
                     PCSSRGVGSSYTRTVASADWGSCCFCTIRRMMNGPGGHQELEVSCPASEAGPAFHLTV
                     DRFPRGNIISKGVVTISRWPSIFGTVVDEKTVACVDGQRYKAQLEAAYPKSATGGNER
                     DWELDVYPFRQCWTFTWSWYRSAPPQISPCGF"
     gene            1130..1624
                     /locus_tag="PLU99_15590"
     CDS             1130..1624
                     /locus_tag="PLU99_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18515.1"
                     /translation="MRASAWLKIGILVVGVGAAVLVPASIHRGRYYRSLNQFYTLLTV
                     GRACLLYQRECGGAPTDVADLLRTGCLRRSANGEWVDTRRGTNGWRTAYEDALRVRLL
                     VPATSASCEWQGGLLIDKATAAPFALVDMDDAAVWREHVDLANKHLAQDWLKMINGEP
                     IPDW"
     gene            1969..>2264
                     /locus_tag="PLU99_15595"
     CDS             1969..>2264
                     /locus_tag="PLU99_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18516.1"
                     /translation="MTNSRATPCVKGNGDGLINSFNVGCLSDAMGNAATRFEWDAENR
                     LTAAYSAFPQAGDKKVTFKYDYLNRRVEKNVYDWTGSAWSATPSQVRRFVYAGHL"
BASE COUNT          425 a          649 c          772 g          418 t
ORIGIN      
        1 gtggccaggt gaacagcctc gaaggcgccg gcgggatcgg cggcctgctg gcggtgtacg
       61 atctgaacga cacgccgggc gccgaacaga ccggcgacga tctccaatac gtgtatacgt
      121 atgatggcaa cggcaacgtg gtgcaggtgc tggactgggc ggcatcctcg gcggtcgagg
      181 cgataaaggc ccgctatgaa tacgacccat atgggaacgt cgtcgcgcag ggcggcgact
      241 acgccgagcg taaccccttc cgcttcagca cgaagtactg ggacgacgag acggggctgg
      301 ggtactgggg ctaccggtac tacagttcgc ggatggggcg gtggattagt cgggatccga
      361 ttttcagaag tgaatctccc aacctgaacg ccgcgctgca caacgatccc ttgagctgga
      421 ttgatccgga cgggcgtcaa gagcactggg gcggtccggt ttcggagccg ccgctgccgc
      481 tgccgccgac ctcgggccct gccagccagc cagcggttga caagtcgaag gcctgctgcg
      541 tctaccgaga ggtcagctcg agtgcggatt cgcacggaat gggcgtacac actaacacgt
      601 gctacaaggt ccatcgatgc ccgcctggtg caacgagtcc tgggcagtgc tgctgcccgt
      661 gctcgtctcg cggcgtgggc agttcttaca cgcgcacggt cgcgagtgcg gactggggaa
      721 gctgctgctt ctgcacaatc aggcgcatga tgaacggtcc tggcgggcat caggagctcg
      781 aggtgagttg tcccgcctcg gaggcgggac cggccttcca tcttaccgtt gatcgttttc
      841 cgcggggaaa catcatcagt aagggcgtcg tgacgatttc gcggtggcca agcatcttcg
      901 gtaccgtggt cgacgagaaa accgttgcgt gtgttgacgg acagcgctac aaggcccagc
      961 tcgaagccgc gtaccccaag tcggcgaccg gcggaaatga gcgcgattgg gagttagacg
     1021 tttacccgtt tcggcaatgc tggaccttca cctggtcttg gtaccggtcg gctccgccgc
     1081 agatttcgcc ttgtggattc tagttgcgag ctctaggtgt cgaatgcata tgagggcctc
     1141 ggcttggctt aagatcggca ttcttgtcgt cggcgtcggt gcggccgtgc tagtgcccgc
     1201 gagcatccac cggggccgtt actaccgctc cctcaaccag ttctacacgc ttcttaccgt
     1261 cggacgcgcg tgtttgcttt accagcgcga atgcggaggt gcgccgaccg acgtggcgga
     1321 cctgctccgc accggttgtc taaggcggag cgcaaatggt gagtgggtcg acacgcggcg
     1381 tggtacaaat ggctggagga cagcgtacga agatgcgctg cgcgtcagac ttctggttcc
     1441 agcaacctca gcctcgtgtg aatggcaggg cggtctgctg atcgacaagg cgacggcggc
     1501 gccgtttgcg ctagttgaca tggatgatgc ggctgtctgg cgtgagcacg ttgacttggc
     1561 aaacaagcac ctggcgcaag actggcttaa gatgatcaat ggagagccca tacccgactg
     1621 gtgacaatcg cgcggatgcc gggcacccga aaagtccgca cgggagggag gcgcatgtgc
     1681 ccgcggctat gaactgcaat ggtgttggcg ctgacccttg acacgcccgg caccgcggcg
     1741 cggtgcggac cgctttgcgg ttcgcggggc ttcggcggaa cgggcgtgtc aagggctgag
     1801 caatccggcg caatatgcgc agcagtatcc ggagtgcgac atcatgcggg ccgacctgaa
     1861 cccttgacac gcggcaattt ctgacgcctg aggcgtcaga gtcgctcgca ccgagttggt
     1921 gagacagcgc aggcgggcgt aagatgacag gcgataggaa ctggagcgat gaccaactca
     1981 cgagcgacgc cgtgtgtcaa ggggaatggt gacggcctga ttaactcctt caacgttggc
     2041 tgcctgagtg acgcgatggg caacgcggcc acgcgcttcg agtgggacgc cgaaaaccgc
     2101 ctcacggccg cctattcggc gttcccgcaa gcgggcgaca agaaggtcac gttcaagtac
     2161 gactacctga accggcgcgt cgaaaagaac gtctacgact ggacggggtc ggcctggtcg
     2221 gcgacgccgt cgcaggtgcg gcggtttgtg tatgccgggc acct
//
LOCUS       DAOSYS010000385         2260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_36559_length_2260_cov_3.453061, whole genome shotgun sequence.
ACCESSION   DAOSYS010000385 DAOSYS010000000
VERSION     DAOSYS010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36559_length_2260_cov_3.453061"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..470
                     /locus_tag="PLU99_15600"
     CDS             216..470
                     /locus_tag="PLU99_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18517.1"
                     /translation="MAASLTENNLADRFPMALANFDLAALREKILAAYRADDPILTKL
                     REYARLLLTAPAVNQGVRADKRRARLGLQVSRLRAAGEAP"
     gene            652..891
                     /locus_tag="PLU99_15605"
     CDS             652..891
                     /locus_tag="PLU99_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18518.1"
                     /translation="MKLTPAQAKVVRRMREGWVLYRNAPHCQRDVYFLLKGGLGSADA
                     AETPARRTIESLERKGYIYVECRLTPAGWEVELPK"
     gene            888..1253
                     /locus_tag="PLU99_15610"
     CDS             888..1253
                     /locus_tag="PLU99_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18519.1"
                     /translation="MTSPTARTLQWFRKRGWPAQVVERWNQYSKTRHDLFGFVDVVAM
                     DPNSGRLVGVQVTSGEGGNLRARLRKAQAISFFTLWKQANDVEFHGWAKRGGKGKRKL
                     WTLRRLDGNGNELAPENTH"
     gene            1225..1599
                     /locus_tag="PLU99_15615"
     CDS             1225..1599
                     /locus_tag="PLU99_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18520.1"
                     /translation="MNWHPKTPTEEAQLLDEIEKRAVSARISSCTIQVSPQWLLALIA
                     EVRGLRRVLLTMDQVLRLRAREKHCWSNVDGTVKVTRYDGGGGPYIVINATEWSEDSV
                     DELCDRIRAMWKRLEFAPEGKP"
     gene            1596..>2260
                     /locus_tag="PLU99_15620"
     CDS             1596..>2260
                     /locus_tag="PLU99_15620"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOO18521.1"
                     /translation="MRAPDYERGNVKLYCGDNREIMAALPECLRFDAIVTDPPYGIKE
                     ARGKNKSRAKLCNAKDFGYSEWDDEPCSPEQFAAMQARSRWQIIFGGNYIVVPPATCW
                     LVWDKLNGETDFADCELAWTNLPKAVRRIQFRWHGMLRDSHEPRVHPTQKPEPVMDWC
                     LAQLPDGCQSVCDPFMGSGTTGVACVRRGLAFLGIEREPKYFALAVKRIDAAIDQYAL
                     LDPV"
BASE COUNT          468 a          629 c          786 g          377 t
ORIGIN      
        1 cgctcgcgcc gtgcgggcgg ccgccaggga gggcggctgg cccgccggcg acggccgaag
       61 gtgtgagatg gatgtaaaaa aggcggacgt aaaacaggcg gccatcggca tgtgccgagt
      121 gtggcgccag tggatcgccg cagtggcaaa cgtcgaccaa gccgaccggg ggcgcgacga
      181 cggcgcgctg gccgacgccg cgcgcgtgca gcgcaatggc ggcaagcctg acggagaaca
      241 acctcgcgga tcggtttccg atggctcttg ccaacttcga ccttgcggca ctacgagaga
      301 agatccttgc cgcctatcgg gcggacgacc cgatcttgac gaagttgcgt gagtacgcgc
      361 ggctgctgct gaccgcgccc gcggtcaatc agggcgtgcg tgccgacaag cgccgggcga
      421 gactggggtt acaggtgagt cgtctgcggg cggcgggaga ggcgccatga agcgcgaata
      481 cctcgaatgc gacgactgca agcggacctt cgacggcatc catcgtcgca tcggcgagtc
      541 attgcgccgt gctgcctcgc gtaagggatg gttccattcc ccgcccgacc gcgactactg
      601 cgcatcatgc acagcgcgcc gtgtgacggc ggcgacgaaa gcgaggaagc catgaagctc
      661 accccagccc aggccaaggt tgtgcggagg atgcgcgagg ggtgggtgct ttaccggaat
      721 gcgccacatt gtcagcgcga tgtttacttt ttgctgaagg gtggcctggg gagcgctgac
      781 gcagcagaga cgccggcgcg acgtacgatt gagtcgctag agcgcaaggg ctacatctat
      841 gttgagtgcc gcctcacccc cgccggctgg gaagtggagt tgccgaagtg accagcccaa
      901 ccgcccgcac gctgcaatgg ttccgcaaac gcggctggcc cgcccaggtc gttgagcgct
      961 ggaaccagta cagcaagacg cgccacgacc tgtttggttt cgtcgatgtc gtggcgatgg
     1021 acccgaacag cggccggctc gtgggtgtgc aggtgacatc gggcgagggt ggaaacctga
     1081 gagcacggct gcgcaaggcg caagcaatct cattcttcac gctctggaag caggcgaacg
     1141 acgtggagtt ccacggctgg gccaagcgcg ggggcaaggg caagcggaag ctctggacac
     1201 ttagaagact ggacggaaat ggaaatgaac tggcacccga aaacacccac tgaggaagca
     1261 cagttgcttg acgagatcga gaagcgcgcg gtcagcgcac ggatttcgag ctgcacgata
     1321 caggtcagcc cgcagtggct tttggcgctg attgcggaag tgcgcggcct acggcgcgtg
     1381 ttgttgacaa tggatcaagt gcttcgactt agggcgcggg agaagcactg ctggagcaac
     1441 gttgacggca cagtgaaggt gacgcgctac gacggcggcg gcggtccata catcgtaatc
     1501 aacgcgacgg aatggtccga ggacagcgta gacgaacttt gcgaccgcat ccgcgcgatg
     1561 tggaagcggc tggaattcgc gccggagggc aagccgtgag agcacctgac tacgaacgcg
     1621 ggaacgtgaa gctgtactgc ggcgacaacc gcgaaatcat ggcggcactg cctgagtgtt
     1681 tacggtttga cgcgattgtt actgatccgc cgtatggtat caaagaggcg cggggcaaaa
     1741 acaagagtcg tgcgaagttg tgtaacgcca aggactttgg atacagcgag tgggacgatg
     1801 aaccatgctc gcccgagcag tttgcggcca tgcaggcgcg gtcccggtgg caaatcattt
     1861 tcggtggcaa ctacatcgtt gttccacccg caacatgctg gctcgtgtgg gacaagctga
     1921 atggcgagac ggactttgcc gattgtgaac tggcatggac gaatcttccc aaggcggtgc
     1981 ggcgaatcca attccggtgg cacggtatgc ttcgtgacag tcacgagccg cgagtgcatc
     2041 caacacagaa gcccgagccc gtaatggact ggtgcttggc gcagttgccg gacgggtgcc
     2101 agtcggtgtg cgacccattc atggggagcg ggacaaccgg cgtagcgtgc gtaaggcgcg
     2161 ggctggcgtt cttgggaatt gagcgtgagc ctaagtattt cgcactcgcg gtcaagcgca
     2221 tcgacgccgc aatcgaccaa tacgcactgc tggacccggt
//
LOCUS       DAOSYS010000386         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_38179_length_2184_cov_5.239079, whole genome shotgun sequence.
ACCESSION   DAOSYS010000386 DAOSYS010000000
VERSION     DAOSYS010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38179_length_2184_cov_5.239079"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLU99_15625"
     CDS             complement(<1..630)
                     /locus_tag="PLU99_15625"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18522.1"
                     /translation="MVASNVDSGPWTVSRLLAWTRDYFGRQGVESPRLCAELLLAHAM
                     GCPRIRLYTQYEAVPGPDVLERYREAVKAAAAGTPIAYLTGVKEFFSLEFEVTPDVLI
                     PRPETEILVERTIRLLRDSEAGGHAAPGAEAAGGQVALGAEHAAGAQVAPGAGDAAAA
                     EETTGAEGTAGVQAPAGESARLSGRNTTHRILDLGTGSGCIAVALAKNLP"
     gene            complement(748..1179)
                     /locus_tag="PLU99_15630"
     CDS             complement(748..1179)
                     /locus_tag="PLU99_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18523.1"
                     /translation="MPSRQLSRAFSYRLSAFDQTAHGGSARKAIEFYEQALVIEREIG
                     DRRAEGTTLGNLGNAYAALGRAPKAVEFYEQALIIHREIGNRAGEAIASWNMGDEYRK
                     LGELERAATLMEVLVDFEREIGHANAETHAAILAELRKRMK"
     gene            1229..>2184
                     /locus_tag="PLU99_15635"
     CDS             1229..>2184
                     /locus_tag="PLU99_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOO18524.1"
                     /translation="MTSLRARWAGCLLVPLIPIVLMAAPAPARAEVTGEQVKRAIRKG
                     VDYLKSRQGADGRWPFRYYVGGETCLATLALLQAGESVDSRAISNALQHILSLENANV
                     YVTSLKIQVLAHIDPQKYRDQISEAARWLADAQDVQGLWSYDQRGRRFDHSNSQFALL
                     GLHAAAQAGVKINPGVWRNALQGVTETQKEDGGWTYQTRDTSYGSMTAAGVADLLILG
                     SRIGVAREGPFENGQAPRCGSYRTNRALAAGLAWLARNFEAGSNPRRRGQHTYYWLYS
                     VERCGMLSGQRYFGRHDWYREGAEFLVKAQQSDGSWSHDTVDT"
BASE COUNT          364 a          777 c          672 g          371 t
ORIGIN      
        1 cggcaaattc ttggccagcg cgaccgcgat gcagccgctg ccggtcccca agtccaaaat
       61 tctgtgcgtg gtattccgtc cgctcagcct cgccgactcc ccggccggcg cttgcacgcc
      121 tgccgtgcct tctgcgcccg tcgtctcttc cgcggctgcc gcgtcccctg cgcccggcgc
      181 cacttgcgcg cccgctgcgt gttccgcccc caacgccacc tgcccgcccg cggcctccgc
      241 gccgggcgcc gcgtgcccgc ccgcctcgga atcccgcagc agccggatgg tgcgttcgac
      301 cagtatttcc gtctcaggcc ggggaatcag cacgtccggc gtgacttcga attccagcga
      361 gaagaattcc tttacgccgg tgagataggc gatcggcgtg ccggccgccg cggccttgac
      421 cgcctcgcga tagcgctcca gcacatccgg gccgggcacg gcttcgtact gtgtgtacag
      481 ccggattcgc ggacaaccca tggcatgcgc caacagcagc tcggcgcaca gccgcggcga
      541 ctcgacgccc tggcgcccga aataatcgcg cgtccaagcc agcagccggc tgacagtcca
      601 cggcccgctg tccacgttcg aagccaccat aactcgcaat atactagttc gcgaacggcc
      661 aggccaacgc ccgcattgga ccaccaagcc gacgcctaca agggccgcta cgcgcgcggc
      721 gtggcgagag ttttttctag cgccgcctca cttcatccgc ttccgcagct cggccagtat
      781 ggccgcgtgc gtctcggcat tcgcatgccc gatctcacgc tcaaaatcga ccaatacctc
      841 catcagtgtg gcggcacgct ccaactcacc tagtttccga tattcgtcgc ccatgttcca
      901 actcgcgata gcttcgcccg cacggttgcc gatctcgcga tgaatgatga gtgcctgctc
      961 atagaactcg acggcctttg gggcgcggcc gagggcggcg tacgcgttgc cgaggttgcc
     1021 caaagtcgtg ccctctgcgc ggcggtcgcc gatctcgcgt tcaatcacca gtgcctgctc
     1081 atagaactcg atggccttgc gggcggaccc gccgtgcgcg gtctggtcga aagctgatag
     1141 ccgatagctg aaagctcgcg atagctgacg actcggcagc tcgccaggac aggcggcgcg
     1201 acgccccgcc ggttatactg ctcgcggtat gacgtcgctt cgcgcacgct gggccggctg
     1261 ccttttggtc ccgctcattc cgatcgtgct catggccgcg cctgcgccgg cccgcgcgga
     1321 ggtcacgggt gagcaggtca agcgggccat ccgcaagggc gtggattacc tcaaatcccg
     1381 ccaaggcgcc gacgggcgct ggccgttccg ctattacgtc ggcggcgaga cgtgcctggc
     1441 gacgctggcc ctgttgcagg ccggggaaag cgtcgactcg cgcgccatct cgaatgcgct
     1501 gcagcacatc ctctcccttg aaaacgcgaa cgtctacgtg acgtcgctca aaattcaggt
     1561 gctcgcgcac atcgatccgc aaaagtaccg cgaccagatc agcgaggcgg cccgatggct
     1621 ggccgacgcg caggacgttc agggcttgtg gagctacgac cagcgcggcc ggcgtttcga
     1681 ccactccaat tcgcaattcg cactgctggg cctgcatgcc gccgcgcaag ccggcgtgaa
     1741 gatcaatccc ggcgtctggc gcaacgccct gcagggtgta acggaaaccc agaaggagga
     1801 cggcggctgg acctatcaga cccgcgacac cagctacggc agcatgaccg ccgcgggggt
     1861 ggcagacctg ctcatcctgg gcagtcgcat cggcgtcgca agagaggggc ccttcgaaaa
     1921 cggccaggcg ccgcgctgcg gcagttaccg caccaatcgt gctttggccg ccggcttggc
     1981 ttggctggcg cggaatttcg aagccggctc caacccgcgc cgccgcggcc agcacacgta
     2041 ttactggctc tattcagtag aacgctgcgg catgttgtca gggcaacgct acttcggccg
     2101 ccacgactgg taccgcgagg gggccgagtt cctggtcaag gcccagcagt cggacggctc
     2161 ctggagtcac gacacggtcg acac
//
LOCUS       DAOSYS010000387         2085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_40418_length_2085_cov_3.356158, whole genome shotgun sequence.
ACCESSION   DAOSYS010000387 DAOSYS010000000
VERSION     DAOSYS010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40418_length_2085_cov_3.356158"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..372)
                     /locus_tag="PLU99_15640"
     CDS             complement(16..372)
                     /locus_tag="PLU99_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18525.1"
                     /translation="MDVVCWIMLASFVGIGHAIAGLLIMYGVRNSKLPDVDGDEHQDC
                     KAHEEILIARLRKANEIISALRAEKDNLRGEMCRKNIEVLRETAPDASPASSGSKTAQ
                     SAARESWTEDGYTARP"
     gene            complement(541..1128)
                     /locus_tag="PLU99_15645"
     CDS             complement(541..1128)
                     /locus_tag="PLU99_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18526.1"
                     /translation="MEIQITISGTSPLILNKFTDAAAMASTNGSRSSAAAGSRGTPRE
                     IAEGKLYYGVDGGLVIPSPNLLRCLVDGGSFFKAGRKQITTKAESMLFSCLDITAAEI
                     PLQHEQPWTVDTRAVVIPATKGRILAHRPRFDDWRLVFTAGLDTSIVQESLLREIVDA
                     AGQRIGLGDFRPARKGPYGRFRVDSWKVVGLKLAA"
     gene            1821..2085
                     /locus_tag="PLU99_15650"
                     /pseudo
     CDS             1821..2085
                     /locus_tag="PLU99_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding oxidoreductase"
BASE COUNT          388 a          705 c          584 g          408 t
ORIGIN      
        1 cgtctctccg ggctgtcacg gacgcgcggt atagccatcc tccgtccagc tctctcgtgc
       61 cgcgctctgc gccgtcttgc tcccgctgct cgccggcgac gcgtctggag cggtctcgcg
      121 tagtacttct atattcttcc tgcacatctc gccccggaga ttatcttttt ccgcccgcaa
      181 ggcgctgata atctcgttcg cttttctgag gcgcgcaatc agaatttctt cgtgcgcctt
      241 gcagtcctga tgctcgtctc cgtcaacgtc cggcagcttc gaattccgga cgccatacat
      301 aatcaggagg ccggcgattg catgaccaat tcctacgaat gaggcgagca tgatccagca
      361 aacaacgtcc attaattctc ctttctacca tattgtccgg ataccacatt cgcatcagcc
      421 atagggctcg ttagaaccct atggatgctg cgtttgcctt gccaatcctt gccgcgccct
      481 gcccagcctg gccttgccaa gccgcgcccc gcccagccca gccccgccag ttcgaaaaat
      541 tcaggcagcc aacttcagcc ctaccacctt ccacgaatcc acgcggaatc tcccataggg
      601 gcctttgcgc gccggacgga agtcgccaag accgattcgc tgcccggcgg cgtcgacaat
      661 ctcgcgcagc agcgactcct gcacgatcga ggtatcgagg ccagccgtga aaacgagtcg
      721 ccagtcgtca aagcgcggcc ggtgcgcgag aatacggccc ttggttgccg gaataacaac
      781 cgcgcgcgta tcgaccgtcc atggctgctc gtgctgaagc gggatttcag ccgcggttat
      841 gtcgaggcac gagaacagca tgctctcggc cttggtcgtg atctgcttcc ggccggcctt
      901 gaagaagctg ccgccatcga ccaggcagcg cagcagattc ggcgacggga tgacgagccc
      961 gccgtcgacg ccgtagtaca actttccctc ggcgatttcc cgcggagtac cgcgcgatcc
     1021 ggcagcggcg ctcgatcgcg atccgttcgt ggacgccata gcagccgcgt cggtgaattt
     1081 gttcaaaatg agcgggcttg tgccgctgat ggtgatttgg atttccatta agcctcctgt
     1141 tgaaaagatt gccttgccac tccttgccta gcccggcctt gccatgcccg gacacgccgt
     1201 tcatcgcctc gcccagccac gcctggacac gccgctatct gctttcgcat tagccggaag
     1261 cctttttcaa agcctccggg tgatgcgctt gccttgccct gccaagcctc gccccgccct
     1321 gccgagccat gccccgcccc gcccggccaa gcaagtacag caaaaccatt ccagccgcgc
     1381 agcagagtga cccggcagcc aaccaaagag actgcctctg atctgctctt gtgtccggcg
     1441 ggaaaagagc ctaaaccgcc ggggccgatc gcaatcgggg ggatgcactg cgcgactgat
     1501 gggcgcatca tcgcaaatgc gcttccttgt gtcaagcgcg tatgcgatgt tttgcgaaaa
     1561 aaaattaccg cccaacgcgg tatcgacgtt atttcatgaa ctgggaacct gccgccaccc
     1621 cgacaactcg gggcgacggc ggttaatccc cggcgcggaa gcgcatccac gtcgttaggt
     1681 ggccaagagc ccgagcggat aactccgccc gggtactcgt aaccaccaaa gccaaccctc
     1741 aaccagttgg cgtgcacgcc agcgcaatca aggcttttgc catgcttacg ctgtgtatca
     1801 ggcttcagtt gagctatcag cagttgctcc gtatcggcgc actgatcgtg ctcgggatgg
     1861 ggcccgacgc cgacccgaaa ctggtcgacg accaggtgat cacgacgacg ctgggcaagg
     1921 aggtcagcga ccccgactca ccggtggccg gccgcgacgt cgctgagctc accgcgatgc
     1981 tgcccgacgg cccgggttac gaacggcgcc tggacatgat gctgcggttg ggaccgttcg
     2041 gggacgggtt cggcgccgac cccgacggcc tgaccctgca gcggc
//
LOCUS       DAOSYS010000388         2045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_41416_length_2045_cov_3.549749, whole genome shotgun sequence.
ACCESSION   DAOSYS010000388 DAOSYS010000000
VERSION     DAOSYS010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41416_length_2045_cov_3.549749"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(661..1230)
                     /locus_tag="PLU99_15655"
     CDS             complement(661..1230)
                     /locus_tag="PLU99_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18527.1"
                     /translation="MNAGCVVMGTAVAAIALLLCAGGPPAGSNGDIEVVHVNVDVTSS
                     GDAALAALAAVRGKQVIADKTMVTTEKRAGGYVVQLFELSKLPASNPVDPAQYGRVTA
                     RIRVAGDGLMLETLRPRSGVSAVREDDFLAALTFLAATGRAPDGAFFMHATESEEGTY
                     VNITRVPLTPGAYTDVVVSGKRIIVWPGA"
BASE COUNT          412 a          670 c          587 g          376 t
ORIGIN      
        1 cgcactcagg gcatgtacca gaaacattgc cagtcaaatc atacccgcag cctgcacaga
       61 gatgcgctgg cctgttcggt tgcaggcgac tggtagcgta gtaaccccct gcggaggcga
      121 ccgctgtgaa gacccaaacc catgccatca tacccagcca cggggtaccg cccaggatac
      181 cgtatgccaa caacccatac gtggctggat tcgccagggc cagcactgcc ggagtgcgcc
      241 acggggcagt ccgcggaaca aagaaggcgc agaccacccc gcaaagtaaa ccaaggacgg
      301 gataggctat caggctggca ccgaacgcct ccgcgcccag gtgcgccggc gtgcttctgc
      361 gcacgagttc ctgagcaaga agggcaaggg caaagctcac cagtccgacg ccacccgtcc
      421 ttagcgtgcc catcatgctg aaggctctcc ggatttagtc gacaaggctt cgtatcctac
      481 atgggcaatc cgggcacttg ggatgccagc taccattggc gtccttcacg cagttctcgc
      541 gccatttgtc caacatcctg ctcgtccact tgttcgcatt gttccacgcg gtctggtact
      601 tggagccgtc gcctttctcc agcgtcgccc cgccaaggag cgttcacgcg atcacccaac
      661 ttacgcgccg ggccatacga ttattcgctt gccggaaacg acgacgtccg tataggcacc
      721 gggcgtaagc ggcacccggg ttatattcac gtaggtgccc tcttccgatt ccgttgcgtg
      781 catgaagaac gccccgtcgg gggcgcgacc tgtggccgcg agaaacgtca acgcagcaag
      841 aaagtcgtcc tcacgaactg cagaaacccc gcttcgcggc cgtagtgttt caagcatgag
      901 cccatcgccg gcaactcgga tccgtgccgt gactcgcccg tattgcgcgg gatcaacggg
      961 attgctggct ggcagtttcg agagctcgaa caactggacg acgtatccgc cggcacgctt
     1021 ctccgtggtc accattgttt tgtcagcgat tacctgcttg ccgcggacag cggccaacgc
     1081 ggccaaggcc gcatcaccgg acgacgtcac gtccacgttc acgtgaacca cttcgatgtc
     1141 tccgttactg cctgcgggcg gcccgccggc gcacaagagt aatgcgattg cagctaccgc
     1201 agtgcccata acgacgcaac cggcattcat cattggcctc cagtcggctg agacgcagca
     1261 ccgcaaaagc gcgctatcat attcttggca tgcgtaatga cgtcgtcaaa ttcctttctg
     1321 ctgctcggcg gcagttcctc tcgaccgccc aggcgcgacc ggcatgccgt cagttccgcc
     1381 cggcatcgcc gacaacgagt atttctacct ataagacgta aactgcgcgc cgcgcgctca
     1441 agaaaattgg aaaaaacttg agaaattgtt tggcgcgctg ggttggcggc ggctgtacga
     1501 tcgcttgacc cctcgacccc ttgatccctc aatccctccc tctggatggt gccgcgaaat
     1561 tccggggcgg cacagaatag tcggatcggg ttcggcaccg gcgcgagatt gttggcggca
     1621 ttccggggcg gcacgccccg gctctgcttg tggtcctgcc tggagatact ggacggtccc
     1681 gccggttcgg agataatcga gaagccgacc ttttatgcgt tccatactga cgggattcat
     1741 ctgtgataga aaaacctgat ccatcggcta gtgcgagcga catgcccgat cccgcggctt
     1801 gtgaatcgaa cccagggcat gcgggcggcc taaggcctca gggcgacctg cccaaccctg
     1861 cggctgctga gtcaaacatg cgtcctcagg gaaacaccgc cggcgacctg ccgaaccccg
     1921 cggctcatga atccaagcct aagcctctgg gcgacggacc gccgccggct catgcggcgg
     1981 ccgcaaagca tccgggcgac ataccggtca gcgagctgcg ggccgcgatg caccaggtgg
     2041 ccgac
//
LOCUS       DAOSYS010000389         2033 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_41701_length_2033_cov_4.586957, whole genome shotgun sequence.
ACCESSION   DAOSYS010000389 DAOSYS010000000
VERSION     DAOSYS010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41701_length_2033_cov_4.586957"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="PLU99_15660"
     CDS             <1..1602
                     /locus_tag="PLU99_15660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18528.1"
                     /translation="YYDRENKTFSALPGADDPQYVQSNPTWSPDGRYVIFARSRAYHL
                     ERDRGEVLLERDECREFLDGEQTFLFDLYRVPFNDGAGGEAEPLAGASQNGMSNFFAR
                     YSPDGRWIVFCRAKSFMLLQPDSELYIIPAEGGEARRLECNTGRMNSWHSWSPNGKWL
                     VFASKANTAYTQLFLTHIDDDGHASPPVLLEQFTSTDRAANIPEFVNVGPAAIRQIRE
                     QFLDDFSYVRAGDAFLAADNDPIGAIARYRQAVQLNPANAVAHSNLGGLLVTQGQVGE
                     GVAHLEEALRLDPENPSAHYNLGMLQFRQGDAKGAIEHLSATVRGDPNIAGAQRVLGG
                     LLCQRGDLEEGKAHLEAALQINPGDAAAHYALGKAFDAQGDVEQAIAHWQQAVDLDPQ
                     DTDSHYLLGKRLAHLNRHAESIQHLTAAVRLRPDSVQLSNDLAWVLVTVPDEKLRDPQ
                     QAVHLARRACEQTHFQAVEPLDLLGVCLAASGRFADAIWAAQEALKLAQAAGHAEVAG
                     MIKARLDLYELGQPYYPPQSPQEGE"
     gene            1636..>2033
                     /locus_tag="PLU99_15665"
     CDS             1636..>2033
                     /locus_tag="PLU99_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18529.1"
                     /translation="MAAAGDNVRVRTFARAGCTEVFASATAPWDTPAGAVIARLERVT
                     ERYSAKLVRTGLHVSAGEPLESHQALGTVPDLVAYVWAVAGVEVKSLAERDDIVANSF
                     SDGQAYYLYADLSPAGKAKPRADQARELFER"
BASE COUNT          375 a          597 c          714 g          347 t
ORIGIN      
        1 tactacgacc gggagaataa gacattttcc gcgctgcccg gggctgatga tccgcagtac
       61 gtgcagagca atccgacctg gtcgcccgac ggccggtacg tgatattcgc ccgcagccgg
      121 gcgtaccacc tggagcgtga ccgaggtgaa gttctgctgg agcgcgacga gtgccgcgag
      181 tttctggacg gcgagcagac ctttctgttt gacctgtatc gcgtgccgtt caacgacggg
      241 gccggcggcg aagctgaacc gctggccggc gcctcgcaga acgggatgag caatttcttc
      301 gcgcgctatt ccccggatgg ccgctggatc gtgttctgcc gcgctaagag cttcatgctg
      361 ctccagccgg atagcgagtt gtacattatt ccggccgagg ggggcgaggc gcggcgtctg
      421 gagtgcaaca cggggcggat gaattcgtgg catagctggt cgccgaacgg gaaatggctg
      481 gtgttcgctt cgaaagcgaa tacggcctac acgcaactgt tcctgaccca cattgacgat
      541 gacggccacg ccagcccgcc ggtgctactg gagcagttca ccagcacgga ccgggccgcg
      601 aacattcccg aattcgtcaa cgtggggccg gcggccatac gccagattcg cgagcagttc
      661 ctggacgact tctcttacgt tcgggccggc gacgcgtttc tggccgctga caacgatccg
      721 atcggggcaa tcgcgcgcta ccgccaggcg gtgcagctca atccggccaa cgccgtggcg
      781 cacagcaact tgggcggttt gctcgtgacg cagggccagg tgggcgaagg tgtggcgcac
      841 ctggaagaag cgctgcggct cgatccggag aacccgagcg cgcactacaa cctgggaatg
      901 ctccagttcc gtcagggtga cgccaagggc gcgatcgagc acctttcggc caccgtccgc
      961 ggcgatccga atattgccgg agcgcaaagg gtactgggcg ggctgctttg tcagcgggga
     1021 gacttggaag aaggcaaggc gcacctggaa gcggcgctgc agatcaatcc gggcgatgcg
     1081 gcggcgcatt atgcgctcgg caaggcgttc gatgcacagg gtgacgttga gcaggccatc
     1141 gcacattggc agcaggccgt ggaccttgat cctcaggata ctgattcgca ctacctgctg
     1201 gggaagcggc tggcgcacct gaaccgccac gccgagtcga tacaacattt gactgcggcg
     1261 gtgcggctgc ggccggacag cgtgcagctc agtaatgatc tggcgtgggt cttggtgacg
     1321 gtgccggatg agaagctgcg cgatccgcaa caggcggtgc acctggcgcg gcgggcatgc
     1381 gaacagacgc atttccaggc ggtcgagccg cttgacctgt taggcgtgtg cctggcggcg
     1441 tcgggacgct ttgcagatgc catctgggcg gcccaagagg ctctcaagct ggcccaggcg
     1501 gcggggcacg cagaggtggc ggggatgatc aaggcgcggc tggatttgta tgagctcggc
     1561 cagccctatt atccgcccca atcgccgcag gagggggaat gagaagacgg gcagtcactc
     1621 ataaggaagc agggaatggc ggcagcaggg gataacgtcc gcgttcgcac gttcgcccgc
     1681 gcaggctgca ccgaagtttt cgccagcgct acggcgccgt gggacacacc ggccggcgcc
     1741 gtaatcgcgc ggctggagcg tgtcaccgag cgttactcag cgaagctggt gcgcacggga
     1801 ttgcacgtga gcgcgggcga gccgctggag tctcaccagg ccctcggcac ggtgccagac
     1861 ctggtcgcat acgtgtgggc cgttgccggc gtcgaggtga aaagcctagc ggagcgagac
     1921 gacatcgtcg ccaacagttt cagtgatgga caggcttact acctttacgc cgatttgagt
     1981 ccggcgggta aggcgaagcc tcgggcggac caggcccggg agctctttga gcg
//
LOCUS       DAOSYS010000390         2024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_41951_length_2024_cov_4.748095, whole genome shotgun sequence.
ACCESSION   DAOSYS010000390 DAOSYS010000000
VERSION     DAOSYS010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41951_length_2024_cov_4.748095"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PLU99_15670"
     CDS             complement(<1..782)
                     /locus_tag="PLU99_15670"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOO18530.1"
                     /translation="MTNPADPNVAVMPDSGSPAGKALPPRLQRAMSRVTEIGSLPEIT
                     TKIVAVVENPRSTAADMHDIVKNDPALATKILKVVNSAFYGLPSQIASLDRAIIMLGL
                     SAVKNIALAASLARFFKGDWGSEHFVPTDLWRHCIAVGVCARGLAQASRQPQPDEYFV
                     AGLVHDMGLLVEAQLFPDDFRTLIDSCAATKQDFCQAERELLGADHQAFGLALATKWR
                     FPPVLRYGAGYHHDPRPLQPEFHRVVTLICLADTICCQLQHG"
     gene            1026..1766
                     /locus_tag="PLU99_15675"
     CDS             1026..1766
                     /locus_tag="PLU99_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18531.1"
                     /translation="MQQLERIKPIAQRLLTLQLENERRDAWLWERALRVSRLAQLIGH
                     APELAGRGIDLLAAGAAGLFHCAGWAAQLEQGGVRWWQLLGRPTTDIQRELGAALLME
                     HAGPLLPAKTARLAADAIRQCNRRGTNLIEAQVLAEAESLDDIGAVTVLRQFRQYHAE
                     GRPITQMMATWTRQQEYRYWELRISDFRFETTRALARARFEALGAYFLALTRDVNGTD
                     VLEVLDKAGVDVPPHLRATIGSEDERPG"
     gene            1817..>2024
                     /locus_tag="PLU99_15680"
     CDS             1817..>2024
                     /locus_tag="PLU99_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOO18532.1"
                     /translation="MEADLARILLRREEIQARVRAMAAEIAAGYEGHQAGLTIVPILS
                     GSIVFLADLIRELPVKMKIAMVHVS"
BASE COUNT          385 a          610 c          655 g          374 t
ORIGIN      
        1 aagccgtgtt gcagttggca acagatggtg tcggccaggc agattagagt gacgacgcgg
       61 tggaattcgg gctgcagtgg gcgcggatca tggtggtagc cggccccgta gcgcagcacc
      121 ggcgggaacc tccatttggt cgccagcgcc aggccaaagg cctggtgatc cgcgcctagc
      181 aattcccgtt cagcctggca gaagtcctgt ttggtcgcgg cgcacgagtc aatcaaagtc
      241 cgaaagtcgt ccgggaaaag ctgagcttca acgagcagcc ccatatcgtg tacgaggccc
      301 gcgacaaaat actcatccgg ctgcggctgg cggctggctt gtgccaggcc gcgcgcgcaa
      361 acgccgaccg cgatacagtg ccgccacagg tcggtgggaa caaagtgctc ggagccccag
      421 tcacccttga agaagcgcgc cagcgaagcc gccagagcga tattcttgac cgcactcagg
      481 ccgagcatga tgatcgcgcg gtcgaggctg gcaatctgcg agggcaatcc gtagaaagcc
      541 gagttgacga ctttcaaaat cttagtcgcg agcgcggggt cattcttgac tatgtcatgc
      601 atgtcggcgg cggtggagcg cggattctcc acgacagcga cgatcttggt cgtgatctca
      661 ggcagcgagc cgatttcggt cacccgcgac atggctcgct gcaagcgcgg cgggagcgct
      721 tttcccgcag gcgagcccga atcgggcatg accgccacgt tcggatcggc cgggttggtc
      781 attatctgcc ctcgaccttc tctggaatat ccactttttc tatttcgggc ggaacgccgc
      841 gttacattat ccggcgacga tggttgccat cgctgaaggc gacgcccgtg ttagccgcga
      901 gcccggtaaa tccttgccgc agacgttcgg caccgataaa ctgccgccaa agccgcggtg
      961 gataacgctc gctgccggcg accgcagaaa acaacccgcg gttcgaataa cgacaaggta
     1021 caggcgtgca gcaactcgaa cggatcaaac ccatcgcaca acgcctgttg acgttgcagc
     1081 tcgaaaatga gcggcgcgat gcgtggcttt gggaacgcgc cctgcgggtc agccgcctcg
     1141 cccaactcat cggacacgct cccgagttgg ccgggcgcgg catcgatctg ctggccgcgg
     1201 gcgccgcggg tttgtttcac tgcgccggct gggcggcgca gctcgagcag ggcggcgtcc
     1261 ggtggtggca gttgttgggt cggccgacta cagatatcca acgtgaactc ggggcggcct
     1321 tgttgatgga gcatgccggg ccgctcctgc cggcgaaaac agcccgcctg gcagcagacg
     1381 ccattcgcca gtgcaaccgc cgcgggacca acctgatcga agcccaggtg ctggcggagg
     1441 cggaaagtct ggacgatatc ggtgccgtta ccgtgctgcg gcaatttcgc caataccacg
     1501 ccgaagggcg gccaataact cagatgatgg ccacctggac gcggcagcag gagtaccgct
     1561 attgggagct gcgcatttcc gatttccgat ttgaaacgac gcgggcgctg gcccgcgcac
     1621 ggttcgaggc tctgggcgcc tattttctgg ccttgacgcg cgacgtgaac ggaacggacg
     1681 tgcttgaagt cttggacaaa gcgggcgttg acgtgccgcc gcatctgcgt gctaccattg
     1741 gttccgaaga cgagcgaccc ggctgatgaa cccggtccct tccatttcgc acgacttccg
     1801 tggatcgtcc ggccccatgg aagccgactt ggcccgaatt ctgctgcgcc gtgaggagat
     1861 tcaggcccga gttcgggcaa tggcggcgga gattgcggct ggttatgagg gccatcaggc
     1921 cgggctcacg attgtcccaa ttctctcggg ctcgattgtc ttcttggcag acctcattcg
     1981 cgagctgccg gtgaagatga aaatcgccat ggtgcacgtc tcga
//
LOCUS       DAOSYS010000391         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_42395_length_2008_cov_4.385561, whole genome shotgun sequence.
ACCESSION   DAOSYS010000391 DAOSYS010000000
VERSION     DAOSYS010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42395_length_2008_cov_4.385561"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLU99_15685"
     CDS             <1..472
                     /locus_tag="PLU99_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOO18533.1"
                     /translation="LPLARRAMRVTMLAGEESRRLGHSYVGTEHLLLGLLREEDGAAA
                     QVLIRLGVKLEAVRAEVVKLSARDSEPAASFEQVTSEPPEKRRVDEFARVDPLGRLLS
                     DAVMELASRKEKAAWRGQHELAGQMFEMAEMIRGILQRLAGAAGRDEGQGPSGA"
     gene            complement(512..1051)
                     /locus_tag="PLU99_15690"
     CDS             complement(512..1051)
                     /locus_tag="PLU99_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOO18534.1"
                     /translation="MWLSGLIGSRYDGLTNFHVVQPGVLLRCGQPRVQDLEHIRQAHG
                     LRTIICARGGTRHPLRGRWFRRERDYCARHAIQFQHVRLSDTRGIAPQVLDEFVALMR
                     DPLNHPVLVHCEQGYHRTGVLVAAYRLALCRWSIERALAEMEACGFELHRPKRQALVA
                     GWLEWAEARGGSAQSQITR"
     gene            complement(1353..1574)
                     /locus_tag="PLU99_15695"
     CDS             complement(1353..1574)
                     /locus_tag="PLU99_15695"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOO18535.1"
                     /translation="MAEYAVILVHTTSHAVYAERVLKQAGLAARLIPTPRHLSSDCGS
                     AVRIPADQRAAASEALSAAGVEFDRIELL"
     gene            complement(1567..>2008)
                     /locus_tag="PLU99_15700"
     CDS             complement(1567..>2008)
                     /locus_tag="PLU99_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOO18536.1"
                     /translation="FLPDKYEAGTPNAVGLAGLAAGVRYVLERGVDKLRAHEQSLTQR
                     LIDGLRNIRGVHVRGTHDAARQTAVVSFTMDGKSPAEIGLALDEEFEIMCRPGLHCAP
                     RAHLTMGTSPAGTVRLSPGPFTTLADIEQAISAIASLAAAEAHG"
BASE COUNT          355 a          660 c          663 g          330 t
ORIGIN      
        1 gctgccgctg gcgcggcgtg ccatgcgcgt cactatgctt gccggtgagg aatcccgaag
       61 gctcggtcat agctacgtgg gaacggagca tctgttgctg ggattgctgc gcgaggagga
      121 cggcgccgcg gcccaggtac taatccgcct gggagtgaag ctggaggcgg tgcgcgccga
      181 agtcgtcaaa ttgagcgccc gcgattcgga gcccgccgcg tctttcgagc aagtgacttc
      241 ggagccgccg gaaaagcgga gggtcgacga attcgccagg gtcgacccgc tgggacggct
      301 cctatccgac gctgtcatgg agctggcctc gcggaaggaa aaggccgcct ggcgtggcca
      361 acatgaactg gccggtcaga tgttcgaaat ggcggaaatg atccgcggca tactgcagcg
      421 actggccggc gcggccggca gagacgaggg ccaagggccc tccggcgcgt aggcctgtca
      481 ggttcacaat gcctttgtga gccttttcac ttcatcgcgt tatttggctt tgcgcagacc
      541 cgccgcgtgc ctcggcccac tcaagccaac ccgccaccaa ggcctgccgc ttggggcggt
      601 gaagctcgaa tccgcatgcc tccatttcgg ccagggcgcg ctcaatgctc caccggcaca
      661 gcgccaggcg gtaggccgca accagaaccc ccgtgcggtg atagccctgc tcgcagtgca
      721 ccagcacggg gtgattcagc ggatcacgca tcagcgccac gaattcatcg agaacctgcg
      781 gtgctatgcc gcgcgtgtcc gacagccgca catgctgaaa ctgaattgcg tgccgcgcac
      841 aatagtcgcg ctcgcgtctg aaccaccggc cacgcagcgg atgtcgcgtg ccgccgcgag
      901 cgcagatgat ggtgcgcagg ccgtgggcct ggcgaatatg ttcgaggtcc tgcacccgcg
      961 gttgcccaca gcgcagcagc acgcccggct gaaccacgtg gaagttcgtc aggccgtcat
     1021 agcggctgcc gatcaggccg ctgagccaca agcgccactg atgaagactg ggccagcgcc
     1081 gacgtcgcgg cgattggggc tttttcaggt caagaacaac gtcgtcgcgt cgcggcaact
     1141 cagctccaaa gggcgggagt ctgcgagcag gcagttcgcc cccggccagc gctcgaaaac
     1201 gcaagcactg cccgctctca cgccgactgc cagcatatgg ccccggcggt gtttggcaag
     1261 ccgttaaccc tggggcatgc ccgcgcttac agcttgggca tggcacccac cgtcggacgt
     1321 ggcgatacaa gatgtggcgc tacaacattc gattacaaga gttcgattcg gtcgaactcc
     1381 acgcccgccg cgctcagcgc ctccgacgcc gcggcgcgct ggtccgcggg aattcgcacg
     1441 gctgagccgc agtcgctgct caagtgccgc ggcgtgggga tcaagcgcgc cgccaggccg
     1501 gcctgcttga gcacgcgctc ggcgtacacc gcatggctgg tagtgtgcac cagaatgacg
     1561 gcgtactcag ccatgcgcct ccgctgcagc cagggacgcg atggccgaga ttgcctgctc
     1621 gatgtccgca agtgtcgtga acgggccggg cgacaggcgg actgtacccg ccggcgaagt
     1681 tcccatcgtc aggtgcgcac gcggcgcaca gtgcaggccc gggcggcaca tgatctcgaa
     1741 ctcctcgtcc agcgccagcc cgatctctgc gggcgatttg ccgtccatgg tgaacgagac
     1801 cactgccgtt tggcgggcgg cgtcgtgcgt gccgcggacg tgtacacccc gaatgttccg
     1861 caaaccgtcg atcaggcgtt gggttaacga ctgctcgtgt gcgcgaagct tatccacgcc
     1921 gcgttccagc acgtaacgca cgcccgctgc cagcccggcc agaccaacgg cgtttggcgt
     1981 gccggcctcg tatttgtcgg gcaggaaa
//
LOCUS       DAOSYS010000392         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_42870_length_1990_cov_5.105426, whole genome shotgun sequence.
ACCESSION   DAOSYS010000392 DAOSYS010000000
VERSION     DAOSYS010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42870_length_1990_cov_5.105426"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLU99_15705"
     CDS             <1..445
                     /locus_tag="PLU99_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18537.1"
                     /translation="VTPPLVYLFLAWRRRNRFALALAAGVLPFMAGLLLFRATGAYQF
                     GNRYIIELLPLLLLLVAEGVRGRLTNISYVLIVLAIAVNLFGTCRFCQLSWPQTDGAL
                     AWSLGAFVALALLARAVCAIQKQLVGSARRAGPGYKSDHSAPKSL"
     gene            477..>1990
                     /locus_tag="PLU99_15710"
     CDS             477..>1990
                     /locus_tag="PLU99_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOO18538.1"
                     /translation="MFARIHSMALSGIEAVACEVEVDVTGRGWGTPTIVGLPDSAVKE
                     SIDRIRSALHNSGFALPKTRTTINLAPADIKKEGPAFDLPIALGLLLGDDQLRSEDVD
                     QFLIAGELALDGRVRPIKGALSLAMLAKDKGFRGLIVPRENAEEAAVAEGIEVYGVSA
                     LSEAVSFLAGELPLEPTTVDLEALFARAAKYDVDFADVRGQEQVKRAILIAAAGRHNV
                     LMIGPPGTGKTMLAKRLPTILPPMNLQESLETTRIYSACGRLPAQTSLMATRPVRQPH
                     HSISIPALVGGGTVPAAGEISAAHHGVLFLDELPEFQRSVLESLRQVLEDGHVTVARA
                     HSAAKFPADFLLVAAMNPCPCGYFTDPRRPCKCSAPQIDRYLARISGPLLERIDIHVE
                     VPAVPVSALRDMQPGTSSDEMRARVLAAVEIQRRRFGPNSTMTNGRMTSRLLRKYAVL
                     DKEGERILRQAVSELGLSARAHDKVLRVARTIADLDNQEHISATHLSEAIQYRRL"
BASE COUNT          343 a          695 c          583 g          369 t
ORIGIN      
        1 cgtgacccct cccctggtct acctgttcct ggcttggcgc cgccgcaata ggttcgcctt
       61 ggcactggcg gcgggcgtct tgcctttcat ggccggtttg ttgctgttcc gagccaccgg
      121 cgcctaccag ttcgggaatc gttacataat tgaactcttg ccattgctgc tactgctggt
      181 tgcggaaggc gtgcgcgggc gcctgactaa catcagctac gtgctcatag ttctcgccat
      241 agccgtcaac ctctttggca cctgccgctt ctgtcagctt tcctggccac agacagatgg
      301 cgcgctggcg tggtccctgg gcgcgtttgt cgccctcgca ctattagcgc gggcggtgtg
      361 cgcgattcaa aagcaactcg tcggctcagc ccgacgcgcc ggcccaggct acaaatccga
      421 ccactcggcg ccgaaatcgc tttgaccggt tggcccaggg ctgtagaatt cggttaatgt
      481 tcgcccgaat ccacagcatg gccttgagcg gcattgaggc cgtcgcctgc gaagttgagg
      541 tggacgtcac aggccgcggc tggggcacgc cgaccatcgt tggtctgccg gactcggccg
      601 tcaaagagag cattgatcgc atccgcagcg cgctgcacaa ctccggcttt gcgttgccca
      661 agacgcgcac caccatcaac cttgccccag cggacattaa gaaggaaggg ccggcttttg
      721 acctgcccat tgcgctgggg ctgcttctgg gcgatgacca gcttcgcagt gaagacgtcg
      781 accagttcct cattgccggt gaactcgcgc tcgacggccg cgtgcgcccg attaaaggcg
      841 ctctgtcgct ggcaatgctc gcgaaggaca agggctttcg cggtctgata gtgccgcgcg
      901 agaacgccga ggaagccgcg gtcgccgagg gcatcgaggt ctatggcgtc agcgccctct
      961 cggaggccgt ttcgtttctg gcgggcgagt tgccgctcga gcccacaacc gtcgacctgg
     1021 aggccctgtt cgcgcgggcc gccaaatacg acgtcgattt cgccgacgtg cgcggccagg
     1081 agcaggtcaa gcgcgccata ctgatcgccg cggccgggcg ccacaacgtc ctgatgatcg
     1141 gcccgcccgg caccggcaag accatgcttg ccaaacgcct gcccaccatc ctgccgccca
     1201 tgaacctgca ggaatcgctc gaaaccacac gcatctactc cgcttgcggc cgcctgccgg
     1261 cacaaaccag cttgatggcc acacgccccg tgcgccagcc gcatcactcc atcagcattc
     1321 cggccctggt cggcggcgga acggtccccg cggccggcga aatctcggcc gcccatcacg
     1381 gagtgctatt cctggatgag ttgcccgagt ttcagcgcag cgtgctcgaa tcactgcggc
     1441 aagttttgga agacggccac gtcacggtgg cccgcgctca cagtgccgcc aagttcccgg
     1501 ccgacttcct gcttgtggcc gcgatgaacc cttgcccttg cggctacttc actgatccgc
     1561 gccgcccctg taagtgctcc gccccgcaga ttgaccgtta cctggcccgc atctccggcc
     1621 ctctgctcga acgcattgac attcacgtcg aagtcccggc cgtcccggtc agcgcactgc
     1681 gagatatgca gcccggtacc agcagcgacg aaatgcgggc gcgcgtactg gcggcggtgg
     1741 aaatccaacg ccgccgcttc ggccccaaca gcaccatgac caacggccgc atgacttcgc
     1801 gcctgcttcg caagtacgca gtgctcgata aagaaggcga gcgcattctg cgccaggccg
     1861 tcagcgaact aggcctgtcg gctcgcgcac acgacaaagt cctccgcgtc gcgcgcacca
     1921 tcgccgatct ggacaaccaa gagcacatct cggccaccca tttgtccgaa gccatccaat
     1981 accgccgcct
//
LOCUS       DAOSYS010000393         1946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_44121_length_1946_cov_5.325754, whole genome shotgun sequence.
ACCESSION   DAOSYS010000393 DAOSYS010000000
VERSION     DAOSYS010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44121_length_1946_cov_5.325754"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLU99_15715"
     CDS             <1..807
                     /locus_tag="PLU99_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18539.1"
                     /translation="ATLALGPLLGGERDIIATTVDGTLRVLNARNGSTRWEDLLGSGL
                     LAGAICGQRDAAGPPAYVGDRGARVWMLARSGGTLKLVPGGDLLHWSDDSLIAGLRTV
                     MTAGQPPLLVACPTGAYSGRQAGVCVLQAGSLLWRYAPGGAIWATPAIADVTGDERSE
                     IIVASIDATAGHACGVVTVLSSEGRCLLRLELPAPIECSPAVADVNGDNRLELLVADQ
                     SGVLHCYDVGRIGPIEWGLGAGDSHNTRNAENAYRFGQAPSGMQWNWKPQ"
     gene            complement(839..1471)
                     /locus_tag="PLU99_15720"
     CDS             complement(839..1471)
                     /locus_tag="PLU99_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18540.1"
                     /translation="MKASANRSLRAWSAACLTAVAMLVSLTGCATMTYDRIRLGASQS
                     ECRQLLDQDVFHSCNIGFSGSRQDAAGRTDAVVVLLGRDGVVGGKLQATVARPRGVPL
                     VPAALAPAPTFQLRGELDLVALSLAGAGPLDVLRAVLVELMDRPTNLSAEPARELVAA
                     GIVRLMERWPNLNAAAQFPDLADTLERVPSGGVARLGITAQNTFFLEYDG"
     gene            1623..>1946
                     /locus_tag="PLU99_15725"
     CDS             1623..>1946
                     /locus_tag="PLU99_15725"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOO18541.1"
                     /translation="MDPEQVDSVLDEANRLLNAGQPEESLRCLEQLEPEALGEDDRIE
                     YGALRAYALGELGRIAEALALLEPLLEEFPKSARLLGALGVVLSGANELEQACEALQA
                     AIALDE"
BASE COUNT          321 a          608 c          711 g          306 t
ORIGIN      
        1 gcgacgctgg cgctgggccc gctgctgggc ggcgaacgcg acatcatcgc gacgacggtc
       61 gacggaacgc tgcgcgtgct gaacgcgcgc aacggctcga cgcgctggga ggacctgctg
      121 ggctcgggct tgctggccgg cgcaatctgc gggcagcgcg atgcggccgg tccgccggcg
      181 tacgtcggcg accgtggggc gcgcgtgtgg atgctggcgc gttccggcgg cactctgaag
      241 ctggtgccgg gcggcgacct gctgcactgg tccgacgaca gcctgatagc gggcttgcgg
      301 accgtgatga ccgccggcca gccgccgctg ctggtggcct gtcccacggg ggcctattcg
      361 ggccgccaag ccggcgtgtg cgtgctgcag gccggctcgc tgctgtggcg ctacgctccg
      421 ggtggggcga tctgggccac tccggcgatc gccgacgtga ccggcgacga acgcagcgag
      481 atcatcgtgg cgtcaatcga cgcaaccgcc gggcacgcct gcggcgtcgt gacggtgctc
      541 agttcggaag ggcgctgcct gctgcggctg gaactgcccg cgccgatcga atgctcgcct
      601 gcggtcgcgg acgtgaacgg ggacaaccgc ctcgagctgc tggtcgccga tcaaagcggc
      661 gtgctgcatt gctatgatgt ggggcggatc ggcccgatcg aatggggcct gggcgccggc
      721 gacagccaca acacgcgtaa cgccgaaaac gcttatcgct tcgggcaagc ccccagcggc
      781 atgcaatgga actggaagcc gcaatagcag cggccgcagc ctcagcgaga cgcctggcct
      841 agccgtcgta ttccaggaag aacgtgttct gcgccgtgat tcccaggcgg gccacacccc
      901 cgctcggcac tcgttccagc gtgtccgcca aatccggaaa ttgcgcggcc gcgttcaggt
      961 tcggccagcg ttccatcagg cgaacgattc cggccgcgac cagttcacgt gcgggttcgg
     1021 ctgacaggtt ggtgggccgg tccatcagct cgaccaaaac ggcgcgcagc acgtccagcg
     1081 gtccggcgcc cgcgagcgaa agtgcaacca agtccaattc accgcgcaac tggaatgtcg
     1141 gggccggagc tagcgccgct ggtacaagcg gcacgccgcg cggccgggcg acagtggcct
     1201 gaagcttgcc gccaacgacg ccgtcgcggc ccagcagcac taccacggcg tcggttcgcc
     1261 cggctgcatc ctgccggctg ccgctgaagc cgatattgca ggagtggaaa acgtcctgat
     1321 cgagcaactg ccggcactcg ctttggctgg ccccgaggcg aatccggtca tacgtcatag
     1381 tggcgcagcc ggtgaggctg acaagcatcg cgacggcggt caggcatgcg gccgaccaag
     1441 cgcgtaacga gcgattcgcg ctagctttca tgattgtcat gatgtccggc gcgtgctcgc
     1501 ctcgcaagtc ggcgcggggc gcgtaaagta ctggcgagtc gcgctgtgcg acgtatactg
     1561 cgcagcggaa acagaagacc gggtggctca agtgagcacc ggtcaggttc ttgaggcgtt
     1621 gaatggaccc ggaacaggtc gattcggttc tggacgaagc taaccggcta ctgaatgccg
     1681 ggcagccgga ggaaagtctg cgctgcctgg agcaacttga gccggaggcg ctgggggagg
     1741 acgaccgtat tgaatacggc gccctgcggg cttatgcgct gggagaactg gggcgaatcg
     1801 cggaggcgtt ggcgctgctg gagccgttgc tggaggaatt tcccaagtcg gcgcggctgc
     1861 tcggcgcgct gggcgtagtg ctgagcgggg cgaacgagtt ggagcaggct tgcgaggcat
     1921 tgcaggccgc cattgcgttg gacgag
//
LOCUS       DAOSYS010000394         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_44514_length_1933_cov_4.275293, whole genome shotgun sequence.
ACCESSION   DAOSYS010000394 DAOSYS010000000
VERSION     DAOSYS010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44514_length_1933_cov_4.275293"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLU99_15730"
     CDS             complement(<1..541)
                     /locus_tag="PLU99_15730"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOO18542.1"
                     /translation="MLGFAMHLSNRKAVIIGVLVLSLPLCVWADDRSTKVSFTAADGY
                     WIYADYYPSVEEYRNGAEDAPIVVLIHDFGADRNSWLPLIEPLRHIGAAVLALDLRGH
                     GESATEDTRERAQRADPELFREMQSDLRGAYDWLASQGNVDRARFSIIAAGSGAAVAL
                     QYAAKDRSVDVVVCLTPPWD"
     gene            727..1278
                     /locus_tag="PLU99_15735"
     CDS             727..1278
                     /locus_tag="PLU99_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOO18543.1"
                     /translation="MPDEGKDSFEALAVPHLETVYRVARRLARDEHEAEDLAQETYLK
                     AYKAFGRFEVREFGIRPWLLKILYNTFLNRQARDKKGPRATDQQTLDQVRAAEGYFGP
                     PELDYENLDQEVRRAIDDLPAEFRSVLLLWATMEVSYHEIAEILGIPIGTVMSRLHRA
                     RQQLAEQLEGFAREHRITGPKNE"
     gene            1275..>1933
                     /locus_tag="PLU99_15740"
     CDS             1275..>1933
                     /locus_tag="PLU99_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18544.1"
                     /translation="MTQPEVFRELATYADGEVDEVLRAELDRAVASDPEFQAAVARWQ
                     ALRQCARRAVASEPVPGDLKGRICRALAHQRRLRLFRWWGLSGLALASAALLVLLLVR
                     PTPPAVFPDNGVVVLHPADFARIYEKCGYTYHHSLTEIGERRPAEVAQEVETWSDIDF
                     PVYLPDISSEGFRLAGVCRCFPPERTGIRTVHANYVRGEFSPGTSSPPENVVSLFSMS
                     GR"
BASE COUNT          374 a          553 c          635 g          371 t
ORIGIN      
        1 ggtcccatgg aggggtcaga cagacgacca cgtcaacgct gcgatccttg gcggcatact
       61 gcagcgcgac ggcggcgccg gacccggcgg cgatgatcga gaaccgggcg cgatcgacat
      121 tgccttggct ggcgagccag tcataagcgc cgcgcaagtc ggactgcatt tcgcggaaaa
      181 gctccgggtc ggcgcgctgc gcccgctcgc gggtatcctc ggtggcgctt tcgccgtgcc
      241 cgcgcagatc caacgccaat acggccgccc cgatgtgccg caagggttcg atcaacggca
      301 gccaagaatt gcggtcggcg ccaaagtcgt ggatgagcac gacaataggg gcatcttcag
      361 caccgtttcg gtactcttcg acgctggggt aataatcagc gtaaatccaa tacccgtcgg
      421 cggcagtgaa tgaaaccttg gtcgatcgat catccgccca gacgcagagg ggcaagctca
      481 ggacgagaac gccgataatc accgctttgc gattggaaag gtgcatcgca aacccgagca
      541 tagaattcgt ttggacgcag cgccgcggcg cctgagttca ttgtaaagcc ctctggtggc
      601 acgtgccacc gcttgccgga actggtttcc gggcccaagc acgagcccga tttcgcgttg
      661 gtcgggaatt gtagaggtga gaatcaggtt tcaaaaaggg acgccggcgg aagtcggcgt
      721 gtcgagatgc cggacgaggg caaggattcg ttcgaagcgc tggcagtccc gcacctcgag
      781 acggtttatc gcgtggcgcg gcgactagcc cgtgatgagc atgaggctga ggatctggcc
      841 caagagacgt atctcaaggc ctacaaagca tttgggcgct ttgaggtacg cgagttcggg
      901 attcgcccat ggctcttgaa aatcctctac aacacgttct tgaaccgcca ggcgcgcgac
      961 aagaagggcc ccagagcgac cgatcagcag acgctcgacc aggtgcgtgc cgctgaagga
     1021 tattttggcc cgccggagtt ggattatgag aaccttgatc aggaggtacg gcgagcgata
     1081 gacgatttgc cggccgagtt ccggtcggta ttgctgctgt gggcgaccat ggaagtgagc
     1141 taccacgaga tcgcggaaat tctgggcatc ccgatcggca cggttatgag ccgcttgcac
     1201 cgggcccggc agcaattggc agagcaactc gaaggctttg cccgcgagca tcgaattact
     1261 ggaccgaaga acgaatgact caacctgagg tatttcggga gctggctacg tacgcggacg
     1321 gggaggtgga cgaagttctg cgtgccgagc ttgatcgggc cgtggcgagt gacccggagt
     1381 ttcaggcggc cgtggcgcga tggcaggcgc tgcggcaatg cgcgcggcgc gcggtggctt
     1441 ctgaaccggt cccgggcgat ttgaagggcc gtatttgtcg cgcgctggca caccagcggc
     1501 ggctacggct ttttcgctgg tggggccttt cgggcttagc gttggcttcg gcggcgttgc
     1561 tggtactcct cttagtgcgg ccaacgccgc cggctgtttt tcccgacaac ggcgtggttg
     1621 tgctgcatcc ggccgatttc gcgcgcatct acgaaaaatg cgggtatacc taccaccata
     1681 gccttacgga aataggcgaa cgccggccgg ccgaggtagc ccaggaagtc gagacgtggt
     1741 cagatatcga cttccctgtc tatttgcctg atatcagttc ggaaggcttt cgcctggcag
     1801 gcgtgtgccg ctgtttcccg cctgagcgta cgggcatccg taccgtgcac gccaactacg
     1861 tccgcggaga gttctcgcct ggcacaagct ccccgccgga aaacgtggtt tcgctcttct
     1921 cgatgtccgg ccg
//
LOCUS       DAOSYS010000395         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_46892_length_1856_cov_6.364797, whole genome shotgun sequence.
ACCESSION   DAOSYS010000395 DAOSYS010000000
VERSION     DAOSYS010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46892_length_1856_cov_6.364797"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLU99_15745"
     CDS             <1..307
                     /locus_tag="PLU99_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18545.1"
                     /translation="EDDVRTVILKCDRCGATAQDDKHFLRQVAVGVNWGYGFQAADRK
                     LEAEWCRECLIKTGLCRPQNEADEKIAPPAPPTLEDLVRDIVREEIEQRGVELETTC"
     gene            301..720
                     /locus_tag="PLU99_15750"
     CDS             301..720
                     /locus_tag="PLU99_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18546.1"
                     /translation="MLSDEQLTTIVAEASVGLHFGPTQAEFTERVRGACEAALEADRA
                     ERGKGRPSDDEIKAKWHEIWLGKYLGHGSVECAIEFANWVADRCRCQCQSVSKEEAEP
                     HLDQGPARAEEHKPTRHAQALIAKWNRRAEANWKAWS"
     gene            739..933
                     /locus_tag="PLU99_15755"
     CDS             739..933
                     /locus_tag="PLU99_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18547.1"
                     /translation="MAKTKPLPCPFCGGTKLQLLHRDCGWWFVCKKCDALGHRLGVVL
                     WWFVYAEAKPEDPPEKRSKR"
     gene            1523..>1856
                     /locus_tag="PLU99_15760"
     CDS             1523..>1856
                     /locus_tag="PLU99_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18548.1"
                     /translation="MKHHRADPDGDGSKSFWLPKGTSSRRLWPIHLDAPGAVWLCPGE
                     LKAAGVIAAGRAAVGITGGEGADLPDGLAELLAGRAVAIVADDDQTGRAWLGKALAAL
                     RTAGIDARA"
BASE COUNT          380 a          540 c          655 g          281 t
ORIGIN      
        1 ggaggacgac gtgagaacag taattttaaa gtgcgatcgg tgcggtgcaa cagcacagga
       61 tgacaagcac tttcttaggc aagtcgccgt tggtgtcaac tgggggtacg gctttcaggc
      121 ggcggaccgg aaattagagg ccgagtggtg ccgcgagtgc cttatcaaga ctggactgtg
      181 cagaccacaa aacgaagcag acgagaagat cgcgccgccc gcgccgccga cactggaaga
      241 cctcgttcgc gacatcgtgc gggaggaaat cgaacaacgc ggggttgaac tggagacgac
      301 atgctgagcg acgagcaact gacgacgatt gtagctgagg cgagcgtggg cctgcacttt
      361 gggccgactc aggcagagtt taccgagcgc gtgcgcggcg cgtgcgaggc agcgcttgag
      421 gcggaccggg ccgagcgcgg gaagggacgg ccgagcgacg atgaaatcaa agccaagtgg
      481 cacgaaatat ggctgggcaa atacctgggc cacgggagcg tcgaatgtgc catagaattc
      541 gccaactggg tcgccgacag gtgcaggtgc cagtgccagt cagtgagtaa ggaggaggcg
      601 gagccgcatc tggatcaagg acccgcccga gcagaggagc acaaacccac acgacatgct
      661 caggcgctga tcgccaagtg gaaccgccgc gccgaagcca actggaaggc ttggagctga
      721 accgaaaggc ggtgcgtgat ggcgaagacc aagcccctgc cgtgcccgtt ctgtggcggg
      781 accaagttgc aactactgca ccgcgattgc ggttggtggt ttgtgtgcaa gaagtgcgac
      841 gcgctgggtc acaggctggg ggtggtccta tggtggtttg tgtacgccga agccaagccc
      901 gaggacccgc ccgagaagag gagcaagcgg tgacccccgc cgccatcggc caactcaaaa
      961 ccgccatcga cctgatccgc caggcgatgc tgctgctgga tgcggtgatg caggagatga
     1021 gggaaagtga atctcacgcc tgagcaaatt gcggccggct tggtggtgga catcgccggg
     1081 ctggctgaag gggcggcatc cggcgtagca aaggctacgt ttgacgtagc ttcggatttt
     1141 cttcagattt ttctgctcag tcgttcttta ccggtctaca gtgagcaggc caacgcttcc
     1201 gccggaactc agcccaaccg agcggagggg ccggcgagtt cacgcgctcg gaataccccc
     1261 gaaaaccgcc ggaaacgggg cgccacgcga cgcaaatggc ccgccctgta ccccgacacg
     1321 gccaaaagcg ccagagcccg cgcaaacgcg ttttgacgcc gctgcttacg cccgggagtg
     1381 cgcggcccgg ctgacggcgg acgccgcggc cctggacaag ctctggcgga cgcgggctgt
     1441 agattccgac acggcccgac ggctcggaat cgggataacg gccgacgggc gacactggac
     1501 gtttcctgtc agcgccgatg cggtgaaaca tcaccgggca gacccggacg gcgacggctc
     1561 gaaatcgttc tggcttccca agggaacaag ctcgcggcga ctgtggccga ttcacctgga
     1621 cgcgcccggg gcggtttggc tttgcccggg cgagctcaag gccgcgggcg taattgcggc
     1681 tggccgcgcc gctgtcggaa tcaccggcgg cgaaggcgcg gacttgccgg acgggctcgc
     1741 ggaactgctg gccggccggg cggtcgcaat cgtcgccgac gatgaccaga ccgggcgcgc
     1801 ttggcttggg aaagcgctcg ccgcgctgcg gaccgctggt atcgacgcgc gggctg
//
LOCUS       DAOSYS010000396         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_58270_length_1573_cov_4.999341, whole genome shotgun sequence.
ACCESSION   DAOSYS010000396 DAOSYS010000000
VERSION     DAOSYS010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58270_length_1573_cov_4.999341"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLU99_15765"
     CDS             <1..324
                     /locus_tag="PLU99_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18549.1"
                     /translation="LEGVRGGLLTLVLFVILIALAFQGLGRRLQRVEHAPAQRAMVWA
                     LSVALFMHAMNFLAVSYFGQIIMIWFLILGVAGALTPSQVTTEGYYVRTGLPVVAHMR
                     AGVRA"
     gene            321..1319
                     /locus_tag="PLU99_15770"
     CDS             321..1319
                     /locus_tag="PLU99_15770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOO18550.1"
                     /translation="MSQALHSTPVASACRPNAAVCDVTVIIVSYNTRLLTLQCLRSVL
                     AETRHCSLEIIVVDNASVDGSVEAIARQYPGVTLIANHENRGFAAANNQGLRLARGRY
                     VLLLNPDTVILDGAIDRIVAYADQRPELGIVGCQVLQDEHTVQPTCFAFQTPLNLLLS
                     FSGLSRLWPRNRWLARPEMGWWARDSEREVDVVSGMFMLVRAAALRQVGPMDEGYFVY
                     GEEADWCYRFRRAGWPCCFAPVARILHVHGGGQSTDQRSVPMYVQNQKSVLLFNRKHY
                     GRAAYYAAKAVYVGAMLVRAAAWGMQALLGRHAARHQVRQATAALRFHLLGTEPAK"
     gene            1316..>1573
                     /locus_tag="PLU99_15775"
     CDS             1316..>1573
                     /locus_tag="PLU99_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOO18551.1"
                     /translation="MSARALETYQAPAVAPRDAIACESFTDFDALANLRHDWDRFVER
                     VGGDVYFSFDWCRIWWRHYAYRRRLCVFVFRAAGELVAILPM"
BASE COUNT          251 a          502 c          510 g          310 t
ORIGIN      
        1 ctggagggag tgcgcggcgg actgctgaca ctagtgctct tcgtgattct gatagccctg
       61 gcttttcagg ggctcggacg gcgcctgcag cgggtcgagc atgcgcccgc gcagcgcgcc
      121 atggtgtggg ccctgagtgt ggccctcttt atgcacgcaa tgaacttcct ggccgtgtcg
      181 tacttcggac agatcataat gatctggttc ctgatcctgg gagtggccgg ggcgctgacg
      241 ccgtcccagg tcacgaccga aggctactat gtgcgaaccg gcctgccggt tgtggcgcat
      301 atgcgcgccg gagtacgggc atgagccaag cactgcacag tacacctgtt gcgtcggcct
      361 gccgcccgaa cgccgcagta tgcgacgtga ccgtgattat cgtcagctac aacactcggc
      421 tgctcacgct gcagtgctta cgctctgtct tggcagagac gcggcactgc tcgcttgaga
      481 ttatagttgt tgacaacgct tcagttgatg gttcggtcga ggcgatcgcc cgccagtacc
      541 caggcgtgac gctgattgcc aaccacgaga accgcgggtt cgctgcggcc aacaatcagg
      601 gcctgcggct ggcgcgcggg cgctacgtac tgctcttgaa cccagatact gtgatcctcg
      661 acggtgccat cgaccgcatt gtggcgtatg ccgaccaacg cccggagcta ggcatagtcg
      721 gctgccaggt gctgcaagac gagcacacag tccagcccac ttgcttcgcc tttcagacgc
      781 cgctcaacct gctgctctcc ttcagtgggc tgagccgcct ctggccccgc aatcgctggc
      841 tcgcgcgccc ggagatgggc tggtgggcgc gcgatagcga gcgcgaggtg gacgtggtct
      901 cgggcatgtt catgctcgtg cgggccgccg cgcttcggca ggtcggcccg atggacgaag
      961 gctacttcgt ttatggcgag gaggcggact ggtgttatcg cttccgccgc gcggggtggc
     1021 cctgctgctt cgcgccggta gcccgaatcc tgcacgtgca cggcggcggc caaagcaccg
     1081 accaacgcag tgtgcctatg tatgtacaga accagaaaag cgtactgctg ttcaatcgca
     1141 agcattacgg acgggctgcc tattacgccg cgaaggccgt gtatgtcgga gcgatgctgg
     1201 tccgtgccgc agcctggggc atgcaggccc tgctggggcg ccacgcggcc cggcaccagg
     1261 tcaggcaggc cactgccgcg cttcgctttc atctcctggg aacggagcct gcgaaatgag
     1321 cgctcgtgcg ctggaaacct accaagcgcc cgccgtggcc ccccgcgacg ccattgcgtg
     1381 cgaaagcttc accgacttcg acgcgctcgc caacctgcgc catgactggg accgcttcgt
     1441 agagcgcgtc ggcggcgacg tgtacttctc tttcgactgg tgccgcatct ggtggcggca
     1501 ctatgcctat cgccggcggc tgtgcgtgtt cgtatttcgc gccgccggcg aattagtcgc
     1561 catcctgccc atg
//
LOCUS       DAOSYS010000397         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Phycisphaerae bacterium isolate AMR_MDS_2079
            NODE_61448_length_1512_cov_4.435141, whole genome shotgun sequence.
ACCESSION   DAOSYS010000397 DAOSYS010000000
VERSION     DAOSYS010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864146
            Sequence Read Archive: SRR4236670
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Phycisphaerae bacterium (wastewater metagenome)
  ORGANISM  Phycisphaerae bacterium
            Bacteria; Planctomycetota; Phycisphaerae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 10:32:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,156
            CDSs (total)                      :: 3,111
            Genes (coding)                    :: 3,090
            CDSs (with protein)               :: 3,090
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 21
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 6 of 21
            Pseudo Genes (incomplete)         :: 14 of 21
            Pseudo Genes (internal stop)      :: 1 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Phycisphaerae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61448_length_1512_cov_4.435141"
                     /isolate="AMR_MDS_2079"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026778"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.65 N 135.05 E"
                     /collection_date="2015-05-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..>1512
                     /locus_tag="PLU99_15780"
     CDS             430..>1512
                     /locus_tag="PLU99_15780"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HOO18552.1"
                     /translation="MRFWCRIAACLASVMWVCQAASPCSTFYIPNGGRPVVGQNYDWG
                     FGDGFAIVNQRGLSKTAAVLDVPASWTARYGSVTFNQYGREFPISGMNEAGLVVATMW
                     LEATQYPRPDSRPAVNSLQWIQYQLDNAASVDEVIVSIERLRISPVDPAPLHYLVADR
                     HGQCAVIEFIEGQAVWRVGAGLPACVLTNSTYDDCLAHLKQYAGFGGTGVVSPSQSSL
                     GRFAWAADRLRRYEAQDPERAIEFAFRTLAEIAQDAGRAGTQWTVVYDIAARRIHFHT
                     RGRAQVRTIELGELNFACGRPVTALNLATAAAGDATPAFVEYTREMNRKLIGDACRET
                     PFLKCVPPVLVEMMAAYPETATCEDKL"
BASE COUNT          263 a          429 c          530 g          290 t
ORIGIN      
        1 tcctgcgctt cagccatgaa cgggccgcga cgagtaacga cgtggtgctt ggaataatcg
       61 tgttcgtctg cgcgatctgg agtttggcgg ccagtccgcg tgtgggcaca cgggggcccg
      121 cttacgcgcc gtaacacccg tctacgtaat tgatgggttg accggccgcg ggccttggga
      181 caacacactt gccggcgcat tgaccgaagg cgcgcgccgc cgcaggttgc taccgagact
      241 gtaagcagtg ggagaggtgt gcggcggtgt agccgcacat ctcgcgcggt cggagtgcgg
      301 cgcggttggc gtgcggcgct tttcggaccg agcactgctt aaagctgcgc tttaagcagt
      361 ggcacacgtg ctctgaagtg aaggggtcgg cgcttgcaag tcgagaccgg gcggattaag
      421 ttttgaaaca tgcgtttctg gtgtcgcatt gccgcctgtc tggcttcggt tatgtgggtg
      481 tgccaggcgg cttcgccatg ctcaaccttc tacataccca atggcgggcg gccggtggtg
      541 gggcagaact acgactgggg gttcggcgac ggtttcgcga ttgttaatca gcgcggcctt
      601 tccaagacgg cggcggtcct ggacgtgccg gccagttgga cagcccgata cgggagcgtg
      661 acgttcaacc agtatggtcg cgagtttccg atcagcggga tgaacgaggc cggattggtg
      721 gtcgccacga tgtggcttga ggctacgcag tacccccgtc ccgacagccg gccggcggtc
      781 aactcgctgc agtggattca gtatcagctt gataacgcgg cgagcgtcga tgaagtcatt
      841 gtcagcatcg agcgcctccg catcagtccg gtcgacccgg cgccgctgca ctacctggtg
      901 gcggatcgcc acgggcagtg cgcggtcata gagttcatcg aggggcaggc ggtgtggcgt
      961 gtgggcgcgg gcctgcccgc gtgcgtgctc accaacagca cctacgatga ctgcctggcg
     1021 catctgaagc agtacgcggg tttcgggggc actggcgtgg tttcgccgag ccagagctcg
     1081 ctgggccggt tcgcctgggc ggccgacagg ctgcggcgct atgaagcgca agaccccgag
     1141 cgtgcgattg aattcgcgtt ccgcaccctg gcagaaattg ctcaggatgc cggccgggcg
     1201 ggcacccaat ggactgtggt ctacgacatt gccgctcggc ggattcactt tcacacacgc
     1261 ggccgagcgc aggtccggac gatcgaactg ggcgagttga atttcgcgtg cggacggccg
     1321 gtaacagcgc tgaatctggc gacggctgcg gcgggcgacg caacgcccgc atttgttgag
     1381 tacaccagag aaatgaaccg caagttaatc ggcgacgcat gccgcgagac gccgttcctc
     1441 aagtgcgtac cgccggtgct ggtggaaatg atggcggcct atccggagac ggcaacttgt
     1501 gaagacaagt ta
//
